LOCUS       DAGQWR010000001        67266 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.001, whole genome shotgun sequence.
ACCESSION   DAGQWR010000001 DAGQWR010000000
VERSION     DAGQWR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 67266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.001"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            144..260
                     /locus_tag="MIW95_000001"
     CDS             144..260
                     /locus_tag="MIW95_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HBY3149361.1"
                     /translation="MDDDINMIAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            424..1491
                     /locus_tag="MIW95_000002"
     CDS             424..1491
                     /locus_tag="MIW95_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY3149362.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PNSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1683..2213
                     /gene="rnmV"
                     /locus_tag="MIW95_000003"
     CDS             1683..2213
                     /gene="rnmV"
                     /locus_tag="MIW95_000003"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBY3149363.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMQRIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2384..3253
                     /gene="rsmA"
                     /locus_tag="MIW95_000004"
     CDS             2384..3253
                     /gene="rsmA"
                     /locus_tag="MIW95_000004"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBY3149364.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3319..4590
                     /locus_tag="MIW95_000005"
     CDS             3319..4590
                     /locus_tag="MIW95_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149365.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDSDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEEYEELEEDEEIEDNEESDRDEDS
                     EEEYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRV
                     KVQEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGV
                     SQGESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMP
                     YLGYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGST
                     GFLRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4741..5856
                     /locus_tag="MIW95_000006"
     CDS             4741..5856
                     /locus_tag="MIW95_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBY3149366.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6042..6938
                     /locus_tag="MIW95_000007"
     CDS             6042..6938
                     /locus_tag="MIW95_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBY3149367.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASIVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7008..7805
                     /locus_tag="MIW95_000008"
     CDS             7008..7805
                     /locus_tag="MIW95_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149368.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(7861..9213)
                     /locus_tag="MIW95_000009"
     CDS             complement(7861..9213)
                     /locus_tag="MIW95_000009"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBY3149369.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9432..10310
                     /gene="purR"
                     /locus_tag="MIW95_000010"
     CDS             9432..10310
                     /gene="purR"
                     /locus_tag="MIW95_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBY3149370.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            10466..10744
                     /gene="spoVG"
                     /locus_tag="MIW95_000011"
     CDS             10466..10744
                     /gene="spoVG"
                     /locus_tag="MIW95_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBY3149371.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            10954..12333
                     /gene="glmU"
                     /locus_tag="MIW95_000012"
     CDS             10954..12333
                     /gene="glmU"
                     /locus_tag="MIW95_000012"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBY3149372.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEAEVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSNVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SVVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            12428..13378
                     /locus_tag="MIW95_000013"
     CDS             12428..13378
                     /locus_tag="MIW95_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBY3149373.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      14218..14303
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14465..14986
                     /locus_tag="MIW95_000014"
     CDS             14465..14986
                     /locus_tag="MIW95_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBY3149374.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNDEGVTLTEAQSAKLLSDIGENKIYTSVTADNLGNPLTSNTKVDNKVLY
                     IVLIDNTVMDSTK"
     gene            15137..16813
                     /locus_tag="MIW95_000015"
     CDS             15137..16813
                     /locus_tag="MIW95_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBY3149375.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            16825..18033
                     /locus_tag="MIW95_000016"
     CDS             16825..18033
                     /locus_tag="MIW95_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBY3149376.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            18048..19760
                     /locus_tag="MIW95_000017"
     CDS             18048..19760
                     /locus_tag="MIW95_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBY3149377.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETHDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            19753..20661
                     /gene="pilO"
                     /locus_tag="MIW95_000018"
     CDS             19753..20661
                     /gene="pilO"
                     /locus_tag="MIW95_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBY3149378.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            20688..21254
                     /locus_tag="MIW95_000019"
     CDS             20688..21254
                     /locus_tag="MIW95_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBY3149379.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            21291..21818
                     /locus_tag="MIW95_000020"
     CDS             21291..21818
                     /locus_tag="MIW95_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBY3149380.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            21839..23377
                     /locus_tag="MIW95_000021"
     CDS             21839..23377
                     /locus_tag="MIW95_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149381.1"
                     /translation="MRKWNKFKSERGAALVLVLIVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEKNIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            23477..24559
                     /locus_tag="MIW95_000022"
     CDS             23477..24559
                     /locus_tag="MIW95_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBY3149382.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESIFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            24540..25322
                     /locus_tag="MIW95_000023"
     CDS             24540..25322
                     /locus_tag="MIW95_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBY3149383.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGILFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILIFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            25424..26074
                     /locus_tag="MIW95_000024"
     CDS             25424..26074
                     /locus_tag="MIW95_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBY3149384.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            26088..26648
                     /locus_tag="MIW95_000025"
     CDS             26088..26648
                     /locus_tag="MIW95_000025"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBY3149385.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            26665..30051
                     /gene="mfd"
                     /locus_tag="MIW95_000026"
     CDS             26665..30051
                     /gene="mfd"
                     /locus_tag="MIW95_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBY3149386.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGAENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            30082..31077
                     /locus_tag="MIW95_000027"
     CDS             30082..31077
                     /locus_tag="MIW95_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBY3149387.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            31201..31740
                     /gene="spoVT"
                     /locus_tag="MIW95_000028"
     CDS             31201..31740
                     /gene="spoVT"
                     /locus_tag="MIW95_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBY3149388.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            31860..33473
                     /locus_tag="MIW95_000029"
     CDS             31860..33473
                     /locus_tag="MIW95_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBY3149389.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            33580..35028
                     /gene="mazG"
                     /locus_tag="MIW95_000030"
     CDS             33580..35028
                     /gene="mazG"
                     /locus_tag="MIW95_000030"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBY3149390.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            35117..35395
                     /locus_tag="MIW95_000031"
     CDS             35117..35395
                     /locus_tag="MIW95_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBY3149391.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            35467..35709
                     /locus_tag="MIW95_000032"
     CDS             35467..35709
                     /locus_tag="MIW95_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBY3149392.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            35771..36025
                     /locus_tag="MIW95_000033"
     CDS             35771..36025
                     /locus_tag="MIW95_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBY3149393.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            36026..36559
                     /locus_tag="MIW95_000034"
     CDS             36026..36559
                     /locus_tag="MIW95_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149394.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            36565..36849
                     /locus_tag="MIW95_000035"
     CDS             36565..36849
                     /locus_tag="MIW95_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBY3149395.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            36962..37882
                     /locus_tag="MIW95_000036"
     CDS             36962..37882
                     /locus_tag="MIW95_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBY3149396.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            37972..40344
                     /gene="spoIIE"
                     /locus_tag="MIW95_000037"
     CDS             37972..40344
                     /gene="spoIIE"
                     /locus_tag="MIW95_000037"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBY3149397.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNNEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            40695..42587
                     /gene="pepF"
                     /locus_tag="MIW95_000038"
     CDS             40695..42587
                     /gene="pepF"
                     /locus_tag="MIW95_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBY3149398.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAIAVMNNN
                     DTEDDAEKKQEVDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMSDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESVDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            42701..43666
                     /gene="galU"
                     /locus_tag="MIW95_000039"
     CDS             42701..43666
                     /gene="galU"
                     /locus_tag="MIW95_000039"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBY3149399.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            44166..45770
                     /gene="rho"
                     /locus_tag="MIW95_000040"
     CDS             44166..45770
                     /gene="rho"
                     /locus_tag="MIW95_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBY3149400.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQENRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            45923..46123
                     /gene="rpmE"
                     /locus_tag="MIW95_000041"
     CDS             45923..46123
                     /gene="rpmE"
                     /locus_tag="MIW95_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBY3149401.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            46268..47161
                     /locus_tag="MIW95_000042"
     CDS             46268..47161
                     /locus_tag="MIW95_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBY3149402.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            47179..48027
                     /gene="prmC"
                     /locus_tag="MIW95_000043"
     CDS             47179..48027
                     /gene="prmC"
                     /locus_tag="MIW95_000043"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBY3149403.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            48071..49135
                     /gene="prfA"
                     /locus_tag="MIW95_000044"
     CDS             48071..49135
                     /gene="prfA"
                     /locus_tag="MIW95_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBY3149404.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            49264..49944
                     /locus_tag="MIW95_000045"
     CDS             49264..49944
                     /locus_tag="MIW95_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBY3149405.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            49973..51013
                     /locus_tag="MIW95_000046"
     CDS             49973..51013
                     /locus_tag="MIW95_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBY3149406.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            51030..51479
                     /locus_tag="MIW95_000047"
     CDS             51030..51479
                     /locus_tag="MIW95_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBY3149407.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            51496..51948
                     /gene="rpiB"
                     /locus_tag="MIW95_000048"
     CDS             51496..51948
                     /gene="rpiB"
                     /locus_tag="MIW95_000048"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBY3149408.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            51984..52613
                     /gene="upp"
                     /locus_tag="MIW95_000049"
     CDS             51984..52613
                     /gene="upp"
                     /locus_tag="MIW95_000049"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBY3149409.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            52913..53728
                     /locus_tag="MIW95_000050"
     CDS             52913..53728
                     /locus_tag="MIW95_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBY3149410.1"
                     /translation="MKNCIAVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            53954..54391
                     /locus_tag="MIW95_000051"
     CDS             53954..54391
                     /locus_tag="MIW95_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBY3149411.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            54576..54803
                     /locus_tag="MIW95_000052"
     CDS             54576..54803
                     /locus_tag="MIW95_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBY3149412.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            54808..55194
                     /locus_tag="MIW95_000053"
     CDS             54808..55194
                     /locus_tag="MIW95_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBY3149413.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAIRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            55197..55901
                     /gene="atpB"
                     /locus_tag="MIW95_000054"
     CDS             55197..55901
                     /gene="atpB"
                     /locus_tag="MIW95_000054"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBY3149414.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            55970..56230
                     /gene="atpE"
                     /locus_tag="MIW95_000055"
     CDS             55970..56230
                     /gene="atpE"
                     /locus_tag="MIW95_000055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBY3149415.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            56353..56850
                     /gene="atpF"
                     /locus_tag="MIW95_000056"
     CDS             56353..56850
                     /gene="atpF"
                     /locus_tag="MIW95_000056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBY3149416.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            56847..57392
                     /locus_tag="MIW95_000057"
     CDS             56847..57392
                     /locus_tag="MIW95_000057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBY3149417.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            57409..58911
                     /locus_tag="MIW95_000058"
     CDS             57409..58911
                     /locus_tag="MIW95_000058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBY3149418.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            58939..59817
                     /gene="atpG"
                     /locus_tag="MIW95_000059"
     CDS             58939..59817
                     /gene="atpG"
                     /locus_tag="MIW95_000059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBY3149419.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            59830..61224
                     /gene="atpD"
                     /locus_tag="MIW95_000060"
     CDS             59830..61224
                     /gene="atpD"
                     /locus_tag="MIW95_000060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBY3149420.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            61227..61487
                     /gene="atpC"
                     /locus_tag="MIW95_000061"
     CDS             61227..61487
                     /gene="atpC"
                     /locus_tag="MIW95_000061"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBY3149421.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            61949..62329
                     /locus_tag="MIW95_000062"
     CDS             61949..62329
                     /locus_tag="MIW95_000062"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBY3149422.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            62344..62805
                     /locus_tag="MIW95_000063"
     CDS             62344..62805
                     /locus_tag="MIW95_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBY3149423.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            62826..63416
                     /locus_tag="MIW95_000064"
     CDS             62826..63416
                     /locus_tag="MIW95_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149424.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            63431..64588
                     /gene="alr"
                     /locus_tag="MIW95_000065"
     CDS             63431..64588
                     /gene="alr"
                     /locus_tag="MIW95_000065"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBY3149425.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            64621..64908
                     /locus_tag="MIW95_000066"
     CDS             64621..64908
                     /locus_tag="MIW95_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBY3149426.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            64877..65260
                     /locus_tag="MIW95_000067"
     CDS             64877..65260
                     /locus_tag="MIW95_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBY3149427.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            65431..66258
                     /locus_tag="MIW95_000068"
     CDS             65431..66258
                     /locus_tag="MIW95_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBY3149428.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            66251..67192
                     /locus_tag="MIW95_000069"
     CDS             66251..67192
                     /locus_tag="MIW95_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBY3149429.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGINAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
BASE COUNT        27184 a         7153 c        12563 g        20366 t
ORIGIN      
        1 tatattataa aaaaagagaa aaataaaagg taaattgttt ctggataact taaaattgga
       61 agtaagaata tttaactaga gaagaattat ttaagttcag gttagctcta ggagtagctg
      121 attttatagg agcaatagat tttatggatg atgatataaa catgatagca attagaccag
      181 aagatataag ggtagaattt aatagagatt tcaaagaaat tatagacctc aaactcagac
      241 cgtatcgata taaaaactaa agctagatga tatagtgttg tatataggac catcagctgt
      301 tatggaagta ttagtgcttt taacaaatat aatagtaaaa cttttgtatg aattgataat
      361 aaaaaaatta aaaaccaaga cataaaaatg gcaacaaaaa gaagttgaat aagggaggta
      421 aaaatgaaat ttaagaagtt agtctctttg ggaatattat caacactttt attgagtgta
      481 ataactggat gtagtaatgt ttcatcaggt tcaaacttaa agtctaataa taagacaaaa
      541 ggagagttaa cagtttatac agcaattgag gaagatagta tagatacata cttagctaca
      601 tttaaagaga aatatccaga tattaaattg aatgtactta gattatcagt aggaggtata
      661 acagcaagat tattagcaga aaaagataat cctaaagcag atgtgttatg gggtgttgca
      721 gctacaagtt tgataatttt agatgacaaa gaaatgctag agccatacgc tccgaaaggc
      781 tttgataagg taaatcctgc atttaaagat gataagaaag ttccttcttg ggttggaata
      841 gatgcttgga tgactggtat aacagtaaat actaaagaat taaaagctaa aaacatatca
      901 gctcctaatt cttatgaaga tttaataaag ccagaatata aagatcttat atctatgcct
      961 agtccagctt cttcaggaac aggatattta acagtatcag aactcataca aattatgggt
     1021 gaagaaaaag catggcagta tatggataaa ttgcataaaa atattggtgt atacactcag
     1081 tctggttcag cgccagctat attagcagct tcaggagaat atcccattgg tatatctttt
     1141 ggaaatagag gtataaagtt aaaaggtgaa ggttatccag tagaagtagt atttccaaaa
     1201 gaaggttctg gatgggagat agaatcaaat gcacttgtga aaaaagataa tataaaagaa
     1261 gcatcaaaag tatttttaga ttgggctata agtaaagatg caatgaatga gtattctaag
     1321 aattttgcag ttactacaat tgacacggga aatccggtgc cagaaggatt tccaaagaat
     1381 ccattagaac agatgataga taatgattta aaatcagcag caagaaatag agaggctata
     1441 ctaaataaat ggatagaaaa atatgatgga aagacagaaa aagagagcta agccttacaa
     1501 taaaatttag tacaaaaagc gcctagaata taagttttac atctaggctc ttttgatata
     1561 tttatttaaa atgcatattt ctatataaaa ataaatagtt tagtaccttg attaaattaa
     1621 ataaaaacct taaaataaag agatgtaaag aaaaactaaa aacgaataaa aggtgaattt
     1681 atatgataaa agaaataata gtagtagagg gtagagatga cgttacagca gttaagagag
     1741 caatagatgc agaacttata actacaggtg gatttggatt tccaaaaggt gtaatgcagc
     1801 gtataaaagc tgctaatgaa agacgtggtg ttataatatt tacagaccca gattttgcag
     1861 gtgaaaaaat aagaaaaaaa atagcatcag aagttccagg atgtaaacat gcatttctac
     1921 caagagagga agctaaaaag gatggagata taggtattga gaatgctact cctcaaagta
     1981 taatagcagc tctgaataaa gttagaactg aaaatacaga aaaaagagat gaatttaagc
     2041 aagttgactt aattagaaat ggtcttatag gtaatgaaga agcatcactt agaagagatg
     2101 cacttggaaa aatacttgga attggatatg gaaatgcaaa acaattttta aatagattga
     2161 ataattatgg agttgaaaga gaagaattta tagatgcatt aaaacaaatc taaacgtaat
     2221 gtttaatgta tgaaaaatat gtatataaaa taaattgtgg aattacggtt aatttaagat
     2281 aaattattta taatatatac cttattttaa atcaatgtaa atacatataa ataagtataa
     2341 ttaataaaat taaatatcaa aataagttaa aaaggagtaa ataatggata gactatcttc
     2401 acataatgca acaaaggaag tagtacaaaa atataatttt aaattttcaa agtctttagg
     2461 tcaaaatttc ttgatagata gtaatataat tgataaaatc ttatctggag ctagaatcac
     2521 aagaggagat aatatcatag aagtaggacc tggtatagga actcttactc gtgagatggg
     2581 taaaatagca gaaaaagtag tggctataga aatcgataga aatttaattc ctatattgaa
     2641 agataccttg tcagatttag ataatacaga ggttgtaaat caagatatac taaaagtaga
     2701 catacaggaa ttagtaaaag ataaattaaa tggtggacca gttaagcttg tagcaaatct
     2761 tccatattat ataactactc caatagttat gaaattttta gaggaagaca tacctgtaac
     2821 agacatagta gtcatggttc aaaaagaagt tgcagataga atgaatgcta ttccgggtac
     2881 taaagattat ggggcattat ctatagctgt tcaatactat tgtgatacag agattgtggc
     2941 aaaagctcca agacatatgt ttataccaca accaaatgta gattccacag ttataggttt
     3001 acatgtaaga gataaaagaa aatatgatgt tcataatgaa gacatatttt ttaaaacagt
     3061 aaaagcatcc tttggacaaa gaagaaaaac tcttcttaat tcgttgggag gtcttggttt
     3121 tcttaataaa gatgaaataa gagaaatatt aaaagaagct aatatagacg aaaaaagaag
     3181 gggtgaaact cttagtatag aagagttttc agttctttca aatataataa atacaaaagt
     3241 atcttctaaa tagaaggtac tttttttaat tttgttaaca tatattgttt agagaaactg
     3301 tgttaagggg ggattgcagt gggttctaaa agaaagttta aaagagacta tataatacta
     3361 gaagctaaag acataaactt tagatacaaa gaacgagtac tgccaaaagc ttttgcaaaa
     3421 gttgaagtaa atgatgaaaa agcaattatt gcattatatg tagaaaatct aaaatatgtt
     3481 aaagatgggt ataaagcagt agcaataaaa gatgaccatg gattaataga cttaggaaat
     3541 ataattttaa atgaacaagg aagaggtgaa tttgttttag atttagaaga ttctgatgat
     3601 gagataaaag gtattgcatt attgtatgaa agaaatatac ctttaattgg atttaaggga
     3661 aataagcttg aaaattatga ggacatatta tttcaagagg tagaggaaga agaatatgaa
     3721 gaatatgaag aatatgaaga gttggaagaa gacgaagaga tagaggataa tgaggaaagt
     3781 gatagagacg aagatagtga agaagaatat attatagaag aatatgatga agatgagtat
     3841 gaagactatt gggaagatga tgaagatatt gaagaaggag agttaaaagc aagaacggaa
     3901 gtagaagatt ctggtgatga ggaaatagaa gagttcaata attataagga aatagaaaat
     3961 tataataggg ttaaagtaca ggaagaatat atagacatag aatatgatga ggaagaagag
     4021 gaagaagaag aagtttttga agtaaataga gaagaagata ggtttgtttc ctcaaaacct
     4081 actaaatcaa agaaagttca aaaagatcag aataatattg aaaaaataaa aagagctaat
     4141 ggagtaagtc aaggagagag tggaaataaa caaaccagta cattacttat gccaagacaa
     4201 ataaaaaaag gtttgaaatt atttaaagaa gtaaaacctt ttgtaacaga ttatatagat
     4261 aagacaagat ggtggaaaat agagataaat cctacaacac tgtgtggata tacaatgcct
     4321 tatttaggat atttaaacgt attgaattac actatgtata gtgatgcagt tttacattca
     4381 tataaataca gacactattt atttggtgtt cagtatgatg aatataataa gagaaaacat
     4441 tatatttatg ctatcccagg aaatagaaat gaacagccag acaatggaag cacaggattt
     4501 ttaagatatc aagcttgtga taatagaaca aatagtttag gatattggct atgttttata
     4561 gattctagac aaagaaatat agttaggtaa aaataataaa aatgaaaaaa tttagactca
     4621 attcttattt tactgaaaaa attaatgaaa tgtagtacaa aactttaaaa actattgata
     4681 atatttaaat attacactac aatatatctt gaaataccta ttatattatg gaggaaaatt
     4741 atgataaacg agcaaagaat tttaaatgag tttcttgaat tagtacagat agatagttta
     4801 tctttaaaag aggggaatgt tgctaaagta ttagttaaaa aattagaaga aataggttgt
     4861 tcagtagtaa tagacaatgc tggtgaaaaa gcaaatggag aaactggtaa tataatagct
     4921 acattaaaag gaaataaaga aggaaagaaa atattattta gttcacatat ggatacagta
     4981 actccaggta taggggtaaa acctatagtg gatgaagcaa atggaataat aaaaagtgat
     5041 ggtactacaa tattaggttc tgatgataaa gcaggtatag cagctatatt agaaggctta
     5101 agatatataa atgaaaataa catagaacat actgatatac aagttgtatt ctctatatgt
     5161 gaggaatgtg gattagttgg agctaaaaat ttagactatg gaaaaataga ttctgaatat
     5221 gcatttatat tagatagtgg tggttctcca ggtgaaataa tagtaaaagc tcctgcacaa
     5281 gatgtaataa atgttaagat attaggaaag actgctcatg caggtcttga acctgaagct
     5341 ggaataagtg ctataatggt agctgccaga gcaatagaaa atatgaattt acttagaata
     5401 gacgaagaaa caactgctaa cataggaatt ataaacggtg gaacagctac aaatatagta
     5461 acaggagaag ttaatatagt tgcagaagcc agaagtctaa aagaaaataa attagatgtt
     5521 caaactaaac acatggtaga gacttttgaa aaagctgcaa aagattttgg agctcaaatt
     5581 gagatagatg tcaatagagc atatacacca atagatgtta gtgaggattc tgaaataata
     5641 aaacttgcaa agaaggcttt ttcaaactta ggtatagaag gtcatactga atctacagga
     5701 ggaggtagcg atacaaatat attaagtaag aatggtatcg aagctataac tttaggaatt
     5761 ggtatgaaaa atgctcatac actaagtgaa catatagcta taaaagactt atatgactct
     5821 gctaaaatgg tagtggagat aataaaggaa gcataaattt aaataatagt gtataatata
     5881 tatatcgcct agcttaagct aggtgatttt tcattttata gaagattgat aatctctatt
     5941 gaaagcaaag agtatacatt tgcatataag agagtgttta atttatgttc agatgtaggt
     6001 tcttaataat atttagattg tttcataagg gaggaataaa tatggaaaca aggtatgaag
     6061 aagcaaataa aataacaata caatcaatac tttggaatgt agtattaaca ataattaagg
     6121 ttatagctgg tgttattggt aattctagcg ctatgattgc agatggatta cattcagcat
     6181 cagatattat aagttctata ggagtactaa taggcaatta tgtatcttct agacctggag
     6241 atagagagca caattatggt catgagaaag cagagacatt agtatcattt gtgttatcaa
     6301 tattgcttat tttcgtttct ataacaatag gtatagaggc aattaaatct ttatttaact
     6361 tagatgcatt aagtgttcca tcaatattac cattagtagt atcagttatt tcaatcctga
     6421 taaaagagta tcaatataga ataacaataa aagttgctaa aaagataaat tcaccagcct
     6481 taaaagctga tgcttggcat cataggtcag atgctttatc ttcagtagct gcttttatag
     6541 gtataggtgg ttctatttta gggtttaagc cactagaccc aattgcgtca attgtagtgg
     6601 ctatatttgt agctaaagta ggaattagta tacttataag ttctgttaat gaattgatgg
     6661 atgtttcagt tgacgaagaa gagataaaag aattaaaatt tattgtagca gatacagaag
     6721 gtgttaaaaa tcttggtgat ataaagacta gaaaacatgg agctatggca tatgtagatt
     6781 tgactatatg tgtggatgaa aatttgacag ttaagcaagg tcatgatatt gctaccaagc
     6841 ttgaaaaaca tataataaaa catatggaat ttgtaaaagg cataacagtt cacgttgaac
     6901 catgtactaa ttgccaagga aataagtgta ataattaatt aaaacttaaa aaaaagtgta
     6961 aaaagttata taattgtttt atgattataa actctagggg tggttaaatg agcaagaaaa
     7021 gaaaactaaa gaaaaaagct gcaatattaa taggagtagt tgttttcttt ataatatact
     7081 ttatatcctt tagaatagga ataaatatgg aaaaggataa aagtgaagtg ccaaagaagg
     7141 tagaaaaaga agaagaagat aataggtctt taaggactca aataaaagaa aagaaaaagg
     7201 tttatatatc agacaaaaat atagaaaaca taaaaattga agaagatatg tgggatgaaa
     7261 taaagttatt tttctcagat tttaagaaag taagaaatac tacttcttct tacgaagcag
     7321 tttatgaagg ttattctgat gatggagtta aattttctac tgaccttaac ttttttagag
     7381 tatatactgt aaataaagaa gaatattata aagtaccagt tagttctaag gatgctttta
     7441 agaaactttt agatgagagt atatatactt catttgacct tttaagtcat tataaagatt
     7501 ggaaagaggt tactataact catggaaaag aaattaaaaa agttcataga tggaaatttg
     7561 atgatttagc acataagatg atttcaaaga gggttgtagg aaaagttcaa cctgagaaaa
     7621 gtaaggaaag aagtgactat aattttacta taaatataaa aggtgataat tttgatgtaa
     7681 aagttgatac aatgggaaaa gattatgtaa aagttacttc taagaaaaat gtatcttact
     7741 atgaggttca tacaagttta tttgattacc ttaaaaatga gatatttgaa attggaaaga
     7801 aataagactt taagaaggct atcaaattag atatttgata gcctaaatta tttttatata
     7861 ttatttttca accaacaagt ctgcaacctt ataaattgga ccagcaccag cagttattac
     7921 taaatcatta tccttaacat tttcacgtaa gtattttact atatcttcaa attcttttat
     7981 atagatggca tctacattgt tttgatataa tttctcaacc aagtccttag aatgtatatc
     8041 tccagggtct ttttccctag cagcatatat atctgttatg ataactttat cagcagcata
     8101 gaatgcctct gaaaattcac ctaaaagaga ttttgttcta gtataagtat gtggctggaa
     8161 gatacaccat aaagttgatt tttttaattt tttggcagca gctaaagttg cttttaactc
     8221 agttggatga tgtgcataat catctactac taaagcattc ttataatacc ctttagtttc
     8281 aaatcttctt ccgacacctt tataaatttt tatatttttt cttatagtct ctaagtcaat
     8341 accagaaaca agagctgcca ttatagcaga agaagcattg tatatgttat gaagaccata
     8401 tacagaaagt tcaaattctc ctaaatctct tccttcatat tctattctaa atattccatg
     8461 accactattg tcaaagtgaa tatcttttat tacagcatca ttgtcagaat ctctaccgta
     8521 ttttataata gtagctttaa catcatacaa tatgtcatca atattttcat catcgccatt
     8581 tattatgaaa tagccttctg ggggtaataa ttttccaaat ttgttaaaag aagccttgat
     8641 ttcatctatt ccagagaaat aatcaagatg gtcttcttca acatttaaaa ctatagatat
     8701 ctttgggtta aaattaagga aactatctac atattcacat gcttcagtta taaagtggtt
     8761 ggaattacct atcttaacat taccacctat catacttaag ttaccaccaa ctagtatagt
     8821 tgggtctaag tctgcatatt caaagattgt agataacata gatgttgtag aagtttttcc
     8881 atgtgttcca gaaactgcta tagaattttt atattccctc atgatttggc ctaaaaaagt
     8941 cgctctgttc attaccaatt tatttttttc tttagcagcc acaagttctt cattgtctgg
     9001 atgaactgct gctgtataaa caaccatatt tacatcatct gatatattat cttttttctg
     9061 accaatataa atagttgccc cttggtccct aagtttgtct aataggtatg attcgtttga
     9121 atccgaacct gaaacttgat aacctttatt gatacatatt tcagctaatg cactcatgct
     9181 gataccgcca attcctataa agtgtatatt cataataaaa ccacctttct ttttaattaa
     9241 gtcctacaat atgaactatt tatgtggtat aataatataa tgttcaatat tttataatta
     9301 atatgcatat aatattatat gtattttatt tttgaatata ataaaacata agtaatatta
     9361 taccacaata agaattgtta tgcattagtt agtttaacgt tatataaagg tacttgtagg
     9421 aggaaataat aatgaaattt aaaagaacag agagaattgg agcaatagtt aaaatactgt
     9481 ctgataatcc aaataaaata tatacattaa gttattttac aaatcaattt aatgcagcaa
     9541 aatcaacaat aagtgaagat ttattagttg taaaaaatgt atttgagaag ctacatttag
     9601 gtaaagttat aactatctca ggagcagcag gaggagtaaa atatatacct aaaacttcta
     9661 tagcagaaaa tgcagagttc ttaatggaat tatgtgagag aatatgtgac aagtctagaa
     9721 tactatctgg tggtttttta tacttgattg acctaattta tgaccctaca atagctgcta
     9781 agataggaaa gatattcgca tcaaatattg agtatgtaga tgcagattat gttgtgacaa
     9841 tggaaacaaa aggtatacct atggcactta tgactgcaaa agctatgaat ttacctctag
     9901 ttataataag aaaagatata aaggtatcag aaggtcctac acttagcatg acatatgtga
     9961 gtggaaacag ctctaaagtt gaaagtatga gtttgccaag aaaagctctt aaaccagata
    10021 gtaaagttat aattatagat gatttcatga ggggtggagg tactataaaa ggaatggtag
    10081 accttatgaa tgaatttggt gcagaggtta taggtactgg agtattcata tctacaacga
    10141 atccttcaga aaaaatggta aaagattata tttcattgat tcaattggat gttaatggag
    10201 ataaaatagt agtagaacca aacttaaaga cttttaaaga tgaatataga aatgaagatt
    10261 tagacgaaga agaagattca gaatttgaat ttgagataga tgaagattaa taaaaaaaat
    10321 atttagaaaa atatgtaaaa ataaaaagag gatatcccta gttgttcata gaattattta
    10381 aaaaacagtt tatattaatt tattccagcg ataataattt taatataaat taatttctgt
    10441 caaaatcaaa gggggatatt ttggtatgaa gataactgac gtaagagtaa gaaaattaac
    10501 agaagaaggt aagatgaaat gtatagtttc aataactttc gataatttgt ttgtagtgca
    10561 tgatataaaa gttatagaag gccataatgg gctatttata gcaatgccta gcagaaaagt
    10621 aggcgaagga aatttcagag atatagctca tccaataaat gcagaaatga gacaagtttt
    10681 agaagatgca gttttacaag catatcatga agcattagtt caatgggaag tagctgctga
    10741 ataataaata tataaaatgg agagagcccg aacatttagg ctctttttaa atgtagaatt
    10801 tctattaaaa tatgtacttt ttagtaaaaa agtgctatat tattagtgat aatgtatatg
    10861 ttgtatacaa attgttagat aaatgtatta taatgtatat tgattaagag aataatacga
    10921 ataaaattaa attagattga atggagtgta tcaatgaact ttaaagctat aatccttgct
    10981 gctggcaaag gaacaagaat gaagtctaaa tacccaaaag ttatacataa agtatgtgga
    11041 aaagaaatgg ttaaccatat tatagatgtt tcaaaaaaat caggggtaaa agatactgtc
    11101 gtgattttag gacatgaagc tgaggtagtt aaagaaaagc ttgcagaaga aattataata
    11161 gccatgcaaa cagaacaact tgggacagga catgctgtaa agatggctaa agaatatatt
    11221 aatgatgaag atacaatagt tgtactttgt ggagatactc cacttataaa agaagagaca
    11281 ctgaagagat tatttgaata tcatatagaa aataaatatc atgctacagt tcttacaaca
    11341 agagttggaa atccaacagg ttatggtaga ataataagag ataagaaggg tgaccttctt
    11401 aaaatagtgg aacaaaaaga tgcaaattca gaagaaaaga tgataagtga gataaactct
    11461 ggaatttatt gttttaatgg aaagagtctt agagaagcac tagatttgct aaataataac
    11521 aattcacaag gtgaatatta tttaactgat actgctaaaa taatgagaga taaaggatta
    11581 aaagttggtg catttgcagg ttcaactatt gaagaactta tgggcgttaa ttcaagagta
    11641 gaattatcaa aggcagaaga aattatgaga cgaagaataa atgaatctca tatggtaaat
    11701 ggagtaacta taatagacac taactctacc tatatagaat ctgatgttat gataggcaat
    11761 gatactataa tatatccagg tgttatgtta caagggaaaa caaggatagg ttcagattgt
    11821 ataattggaa tgaactcatc tataacaaat tcagaaatag gtgatggtac agaaataaaa
    11881 aattcaacta taattgatag taaggttgga gaaaattcta atgttgggcc atatgcatac
    11941 ttaagaccaa agagtgactt aggaaataat gtaaaaatag gcgattttgt agaagtaaaa
    12001 aatgctatta tagaagatgg atcaaaagca tctcaccttt cttatatagg agatgcacat
    12061 gtcggtaaaa atgtaaatat tggatgtgga gtagtatttg taaactatga tggtaaaaat
    12121 aaatttaaat cagtagtaaa agacaatgcg tttataggtt caaattctaa tttagttgca
    12181 ccagtagttg tagaagaaaa aggatatata gcaacaggtt caacaataac acatgatgtc
    12241 ccagatggtg ctttggcaat agcaagagaa agacaagtta tcaaagaagg atgggtagaa
    12301 aaaaagaatc aaaaagacga ccaaagtaag taattatgta gtttattatt ttggctaaaa
    12361 aatttagctc gaaattatta taaattatat tcattagtat tttaaaattt tcaggagggt
    12421 tacataaatg aatactagcg gaagcgagat taaaattatt gcaggtaatt catcgaaaga
    12481 gttagcacaa aaaatagctg actatatagg tgtatcagta ctagattgtg aagtaggtac
    12541 atttagtgat ggtgaaatat gtgtaaacat gaatgagaca gtaagaggtt gtgatgtgtt
    12601 tgtagtgcaa tctactaaca gcccagtaaa tgataactta atggagttat taatcttaat
    12661 tgatgcgtta aagagagcat cagcaggtag aataacagca gtcattcctt actatggata
    12721 tgcaagacaa gacagaaagg ctaaggcaag agatccaatc actgctaaat tagttgcaaa
    12781 tttaataact gctgctggag cagacagagt attaacaatg gatttacatg cagctcaaat
    12841 acaaggatat tttgatatac cattagacca cttactaggt ggaacaatat tagcaaatta
    12901 ttttaatgaa aagaaaatag aagatttggt agtagtatct cctgacttag gaagtgttac
    12961 tagatcaaga aaatttgcta atactttaaa tggagaagtt ccaatagcta taatagataa
    13021 aagaagaccg aaagctaatg tatgcgaagt tatgaactta ataggtgatg ttaaaggtaa
    13081 aaatgttata ctattagacg atatgataga tacagcagga acaatagtta atgctgcaaa
    13141 tgcacttaaa gagtttggag caaaagatgt ttatgcttgt tgtactcatg gtgtgttatc
    13201 aggtccagct attgaaagaa ttgctaattc agaaataagt gagttaatag ttcttgatac
    13261 tatacaactt cctgaagaga aaagaataga taagataaag ataaaaacag tagctccttt
    13321 atttggagat gctataagaa tgatattctc taatgaatca gtaagtaaat tattctaatt
    13381 tattgtattt atgagagttg tttaaaagga tataaacctt ttgagcaact cttttttgat
    13441 tttatactta tggaatgtta tttttttctt gttagaaagg tatatattat tgtaaaaaga
    13501 gattatagtt taaaattatg tatatttaag taaaataaac ttaataaagg gcatattatt
    13561 caaaatatta tgaatataaa aaatattaca aaaaagtatt gaaaaaattc agattatgta
    13621 gtattatgta aatataaata aagaaagagg tgtttccaat gggaaggtta gaagatgaac
    13681 ccctaataat taaatgattc agtcacaatc taaagtgaat ataaaaatct attgaaagtt
    13741 tatttataag aattgaaaat agtatctatt ctatctttag tgtttgtgag agggttctgg
    13801 attatataag attactgtaa agagtaattt tgcacaataa gcatagctgt aaatgaagag
    13861 tttcgtttat ataatgattg tggctgaatc ataactataa tagatagaat gtttataata
    13921 gatgatttta taaaatacta tattgtaata gcttttagct ttcaaataaa tattagcaaa
    13981 ggatggaaat gacaatgata aaactaaatg ataatttata aatatctcct tacgatatat
    14041 aataattgta gggagatgtt tctttttttt gtagagtata atttttctag aaattctgtt
    14101 tcaatatgta aaaagttggt atatataaat agtataaaca gtgttgctaa aatatggtag
    14161 atattatata atataagtgg atatgtatat acattatgag ttatataatt ttgaatttaa
    14221 taaaatagaa acgttgattt atgttctgta atgtgggcac cttagagcat attgagttag
    14281 tggtgcaacc ggctatgaaa ttgtatttat ttatagatac tatcattttc atagcttttt
    14341 ttatttaaaa aatggttatg tgttagaaat aaatttggtt agtacgttta aataatttta
    14401 cttagatttt taaccagata tattataagt aagtattaat taaaaaattt aagggggaat
    14461 aaaaatgaag ttaaaaaaga ataaaaaagg tttcacttta gtggaattat tggtagtaat
    14521 tgcaattata ggtatattag cagtagtggc agttccagct ttatttagta atataaacaa
    14581 ggctaaggta gcaagtgttg agtctgatta tagttcagtt aagagtgcag cattatctta
    14641 ttattcagat actaataaga taccagttac accagatggt caaactggtt taagtgtttt
    14701 agagacttat atggagtctc tgcctgataa agctgatata ggtggaaaat ataaattgat
    14761 taaagttggt aataaattgg tattacagat aggtacaaat gatgaaggag ttacattaac
    14821 agaagcacaa tcagcaaaat tattgagtga tataggtgaa aataaaatat atacaagtgt
    14881 tacagcagat aatttgggaa atccattaac aagtaataca aaagtagata ataaagttct
    14941 atatatagta cttatagata atactgtgat ggactcaaca aaatagttaa aactatattt
    15001 ttataaaaat attgtaaatt taatgaaaaa tataatataa aagacaggaa gaaaatcttc
    15061 ctgttttttt atactatttt ctttaatgtt caattatgaa tgatgatata attgagatag
    15121 taaaggagtg aaacctgtgg ctaaaaaagt tagaatagga gacaaactgg tcgaaaaagg
    15181 atatataaca gaagaccagc ttaaatgggc actatcagaa caaaaaaaca gtggaaaaag
    15241 actaggagaa tttctagtac aagaagggct tatagatagt aacttactaa taagtgtatt
    15301 aaaagaactc ttggatatag aaagtatatt tttagaaggg acagaaatag acactttagc
    15361 tacaaagatg gttccagaaa atatatgcaa aagatatact gtatttccat ttaaaattga
    15421 tggaaataaa atatgtctag ccatgtcaga cccacaagac agagaagcag ttcaagatgt
    15481 aaggcgtatg tctggtaaag acgtagagat atttatatca agtacagaag atataaacaa
    15541 agcaataggt catgcttatg cacattcaga aataaataag gcaatgactg agtacaacaa
    15601 aaatagaact ggtggagtaa gagaaacagt aatacttgaa gaagatgtaa atgctgctcc
    15661 aatagtacga ttagtaaata acatactaga aaatgctgtt agaatggaag caagtgatat
    15721 acatatagaa caaagtgaaa attacatgcg tgttaggttt aggatagatg gaatgttaag
    15781 agaatatatg aggatgaact cagcaccata taaggcagta atcagccgta tcaagataat
    15841 gtcagatata aatatatctg aaaagagaat accacaggat ggtagaatct atttaaaagt
    15901 agataataaa cccatagatt ttagggtttc tacaatgcca actaacaggg atgaaaagat
    15961 agctatgagg gttcttgata agagtaattt tatggttagt aaagaagtac taggaataga
    16021 cgaacatggc tctaagatat atgatgaatt gataaacact ccttatggtc ttatattggt
    16081 tgttggtcct actggaagtg ggaaaaccac tacattgtat tcaatgttaa atcaactaaa
    16141 tacagaaaat agaaatctat taacaataga agaccctata gagtatgaat tacctggtgt
    16201 aaatcaatct caaataaatg aaaaggcagg gcttactttc gccagcggac ttagagcttt
    16261 tatgcgtcaa gacccagata taataatggt tggagagata agagatactg agacagcaga
    16321 aatagcaatt agagcttctc ttacaggaca tttagtttta agtacattac atgcaaatac
    16381 agcagttgga gcaatttcac gtcttttaga tatggatgtg gaaagttttt taataacttc
    16441 atctgtttta ggtgtaatat ctcaaagact tacacgaaaa atatgtgagc attgtaaggt
    16501 ttcatatgaa gcagatattg gagaaaaaaa ggctcttggt attgatgtaa atgaatcagt
    16561 aactatttat agaggaaaag gttgtgaaag gtgtaataat actggatata aaggacgtct
    16621 aggtatattt gaaatgcttg aaattactcc agaaataaaa gagttaatag attcatctgc
    16681 aaatcaaaga gaaattttaa agatggctag aaaacaaggc atggttagct taaaagagga
    16741 tatagttaaa aaggttttaa atggaaagac aacagtagaa gaaatgatta gaataatttt
    16801 gatgactgat taaaggatga tattatgaaa agttttaaat acacagtaat aaaagaggac
    16861 ggaaaaaagc aaactgatgt gattgaagcc aacgatttta atgaagctag aaatcttctt
    16921 agaaagagaa atcttagagt tattgaaatc aaagaaagca aaaataaaaa tataagatta
    16981 tctagtagaa agagaaaaaa agacttggga gctgaccaga taagccactt ttgtagacag
    17041 tttgctatta tagtatcttc tggtataaat agtatttcag gattagaaac attagctaga
    17101 aggtcaacta acattacatt aagagaagaa attaatcgta tagtggcaga aataaaagta
    17161 ggttctacta ttgcagattc aatgctttca tcaaaatcaa agtttccaaa gttacttggt
    17221 gctatggtag ctacaggtga agctacaggt aaattagatg aagtattaaa aagtatggct
    17281 tcattttatg caagtgaaca cagggttaaa caaaagctta gaaatgctgc aacatatcca
    17341 ttaatagttt tgatttcttc atttataatg atttttatat ttacaacatt tatggtgcct
    17401 aagatgatta attcaattac aacagtagga gcaactcttc cacttataac aagaattgtt
    17461 atggggtttg gtatgtttat gaagaagttt tggataatag tacttttagt tattatatta
    17521 tttatctatc agtttaaaaa atatttaaaa acgtcagttg gaagggctca taaagataga
    17581 gttataaaca agatacctgt tttaggaaaa ggaataaatt gtatggtagc tacacgattt
    17641 tcaagagcat tgtatctatt tgtatcaaca ggatacccat tagttcaagg gcttgattat
    17701 attatagata gtgtaaataa caccatggca gagaagattt tagcatcagc taaagatgga
    17761 ataacaagag gagaaggctt agcagaaaat ttagagagat atacgtattt tgactctgtg
    17821 ttggtacaga tgatagctat aggggagcag acaggggaat tagagaatat aagtagacag
    17881 atggcagaat tttatgagta tgaatccgag atttatttaa ataggatggc ctcaatgata
    17941 gagcctgttt taataattgc agttggtatt atagttgcga tactggttgt atcaatattt
    18001 atgccaatgc ttagtatata tgatgccatg taggagggaa aggacaaatg aataaggttt
    18061 atgtatcata ttatggcgat tctatttcca tagttgaggg cagatataaa cacgataagg
    18121 ataagtttta tgtaaaaaat tttaatgtgt tttcaataga ggatattgca caagattttt
    18181 ctaaggaaaa gtatgctcta ttgagatacg cattagaaaa tgttaagtta aaatcaagac
    18241 atgtagtttt ttgtttaaat acaagagatg taattttaaa accccaaaaa cttcctaata
    18301 tggacaaaaa ggatttagat ggatttatga agctagagat ggacgaaatg atggctttgg
    18361 agtcagatga atatgtattt tcgtatgaag tatctagtga aacacatgat aattcagatg
    18421 gcgatgaatc aagcttaaat cttatcattg gagctataca aaaggaagag gtaaatgcaa
    18481 ttgtagagat aatgcatgaa tttgatttag tattagatag aatagataca ttatcaacag
    18541 cttacttaag aatgcttaaa aatttagact acaatgatat aatggtaacc aatataagtg
    18601 acaatagtac aattataaat atatataaga aagatacttt atttattagt gataatatac
    18661 ctataaagat aactgctaaa gatatggaca tgcaattatt aaatgtagta gaagaatcaa
    18721 aaggtcttat gaattactat tcgtcaagaa actttggtaa ggttacggat actatattaa
    18781 tattaggaaa aagatattcc aataaattaa tttatgaaac ttttagctct gcattttcaa
    18841 gctatgtttc tcaaggcctt actgagataa tggatatatc tgatatgata actggagata
    18901 tagatgaaga tgatataaat tcaattgtag agcttttagg aagtatgata gaacctaaag
    18961 ggaaaagaga ttttgaaaat ataaactttt taccaatgga tatacttaga aaacagataa
    19021 aagctaaaca aatgaaaaac atactgaaaa ttgctccaat agttattttg attcttgcta
    19081 taccttattt atgtttaatt ttttcaaata atttagcaaa taaggaactt gaaaatgtta
    19141 atggtgaaat aagaaatatt gaatctgcat attatcagat tggaaatata gaaaaaatga
    19201 tacagagtaa aagcgaagaa ataaaggtat atgatatgtt aattgggaaa aaggtaaagt
    19261 gggaaaattt attggatgat atagataaaa atactccaag taaagttgaa ctaacaagtt
    19321 tatcaacaac ttatcaaaat cttggaacta ataataaagc tgataattca aaaagtaata
    19381 agactacaac agataaatca agtaacaatg aaacaaaatc aagtacgaaa agtgatagta
    19441 atggagataa aacagataat gatttaaatg aagataagac tacaaatgtg tcagaagcat
    19501 taactggaaa tagtgagact aatagtaata acacggataa agatgataaa actccattgt
    19561 atgataagat accaaatgtt ataaatatag aaggaaatgc taaagattcc tcttatgtag
    19621 gtcaattttt atataaatta aagggattat actatttcaa ggatgttaaa ttacatagca
    19681 taaaaagtaa tgcagaagat tctaactata catttagtat gacattatac ctgaaagaag
    19741 gtgtattaac taatgaataa agatatattt aaaaaagata taaaagatat atttaaaatg
    19801 gaagttagtc taaaagggat tcttataaag gttataattt tattagctat tttatcatat
    19861 ggttattttg gactttaccc tcaatatcag aaatatattg ctacaaaaaa ttctgtaaaa
    19921 caggctactg aaaaattaaa tatatataaa gagaaaactg cacttatgcc aaaatatgaa
    19981 aaacaacttg aagaagttga aagtgaatta aaagaaaaaa gcaaaaatct tttatatgat
    20041 atgcaagatg gtcttttttt aataggttta gataaaaaaa tgaaggctta taatattgac
    20101 ttaactagtt atgacgttgg aaatgttaaa gaatacgaac atttttatgg tatatcaaca
    20161 acaataagtg ttagaggtga ttatagaaat gtcaaaaaat taatggctta tttagaagaa
    20221 caaaaaaatg ttactcaaat actagattat acaatgactt catatataga accagaaaaa
    20281 gaagaggaag aaacaacaaa caataaggat ttaattaaaa atgtgaacat aaataaagat
    20341 acaaaaatat actgggtgga aggcgaaaat aagtggcatt tatataagga ttgtccattg
    20401 ataaaagaag gagagattct aaaaaatgac aaacttttag ataggtctac taattatcta
    20461 tgtaatactt gtgtacttag agctgcaaca gaaaaagaag caaaaaaaga agtcgtagct
    20521 ccaaaggcag aaggtgtagt agatgctaca tttaaattta taatgtacac taaagagaat
    20581 actacaatga tacttgatac agataatcca tcttcatgga gaccaggaaa atataatcca
    20641 tttaagtcta gtgctaaata aagctaagta agctttaaag ataggtgatg agtttgtaca
    20701 aaaataatga aaaaggtctt actttattag aagtaataat agctgttttt atactgacaa
    20761 tagttttaag tatttcttat aaagtgttca atggtataac atcagcagta aaaaagcaac
    20821 agattattac agatgctcaa gtaaatatta atctaattaa taagtatcta aatagagatt
    20881 tggaaaactg taaagagcta actaaaactg gttcaggtaa taattatgaa tacaatatag
    20941 agatgccaga taatgttgta aaatatgaag tttctataga aactaaaaaa aatacagagg
    21001 tatattctgt aacgagaatc caaaacaata caattgatac agaaaatgaa gttagagaag
    21061 agataatcta taatcaacca ctagttcaaa acaataaaga aatgaaggaa acaccattta
    21121 aaatagaaaa gcaaactggt aaatctattt atactgtaag tatatactat aatgaatcag
    21181 tgcaagaaag tcataaaaat agcaatttaa ataacaaaac ttatacattt gatgttatgt
    21241 caagaatagg gtaataagaa tttattaaaa aataagtagg ttgataaaat atgagtaaaa
    21301 agaatagtga aagaggattt tccttaattg aagtgttagt agctatggct attatgggaa
    21361 ttgtactatt tgcatttttt aatattatca atactaataa caaagcaaac actaaaaatg
    21421 acacagatat aacttcctta aactatgtcc aaagtgaaat agaaaatcta agagaaaaga
    21481 taaaaagtgg agaatttgat tttgatagtt tagataaact agaagatgga actgtcgtat
    21541 atgaaaaatt aatagataaa tcaaaaaaag ttgtatatga taaagttctt agtgaaggtg
    21601 acgtgagctt atatgatact ccatatgaaa agattacaac aataaaagat gaagatggta
    21661 atttaatcga caaaggaaat attacaaata agataaagac tattgtagaa gataaaagtg
    21721 gtcagatata taaaatagct gtaactggga aaagcatgaa tgactattca agtaaaaaag
    21781 aggttaaaat tgtaactgaa atatttaaag ataaataatt ttacttgggg gtgagatttt
    21841 gaggaagtgg aataaattta aaagtgaaag aggggcagct ttagttttag ttttgattgt
    21901 agtagcattg ctttctattg ttggattgat attttcaaat cagatagcta atagaataaa
    21961 atctacaaag actactaatg aaggtataca agcaaagtat ttagcagaga catgtgtgga
    22021 aaatagtatt gataaagcat atgaaaaatt gtatgatgag cttgagaaaa tggataatga
    22081 gtttaaaagt gaaaatcaag aaaaatccat atctaggtca aaattaagaa atatatcaga
    22141 tgaagatttt aataatcaag atgagaaaaa tattgaagca gaaagacttg gatatatgaa
    22201 taatataaat ttctatttaa ataaagcttc ttcagacttg gaaaaagcta gtatggaatt
    22261 gaaaaagcta tatgatttag atatgttgga ctatagagac attgaatatg tagatgcaaa
    22321 tattatttca catagagatt ctatcttaga aatttgcaag aattatactt ctggagatat
    22381 tagtaaaata aatgaatata ttctgaaaga agatatagac tcaactacat tgattgaggc
    22441 aaagttagtg aataatgata ttcttttgaa aatgttttta gaagaaaata aaattgaaaa
    22501 tgaacattta aattcagcct tttcacatac ttataaggca ttagataaca tttctttggc
    22561 gatgcaaaat atgattgagt atagacatac gtttcatata gatgagccaa aagtagaagt
    22621 atctaatggg attccagatt ctcaacaata ttatgaacta atacaaaatc ctataataaa
    22681 tagtatggag tatatctgga atagtaaatg ggatacatta gaaaacctat tggaaatttt
    22741 accaaatcaa actcaaggat ttaattcttt aagagttcat ttgagaaata atgtaagaaa
    22801 atttgagaag ttaagcgata atatatctag tggtaaaaaa aatacagcaa aaaatttttt
    22861 aaagtataaa gagttattgt atgaaatatc agaccaatgt aatcaactaa aaagtatgtc
    22921 ttatgagaaa atacctgtta aatatgataa tatggctttg ataacaactt ttgactatat
    22981 acaaaatgag cttttagcag agataaaatg cagattaaaa gaattaaaac cacaagaaat
    23041 tgataaaaca gaaggtatta ctataaaaat acctttttat aaggctgact acgacatgac
    23101 aaaagaagga tggcctaaat taaaagaaaa tggttcaggg gcggagttaa gcttaatggt
    23161 aactggtgat aaagatggaa taaaagaagt agaagttaca gatggtaaaa aaaatattat
    23221 tggtttaggt gttgaagaaa attcaaattc aaagtataag gttgacgcca ttgtaaactt
    23281 taatctaaat atagatacaa atgtagttgg aaattatgat ataaaagata aaattttgat
    23341 taatcatgat ataagttcat acaaaaaagt aaactaaata taagaagcaa cttagtataa
    23401 ataaataata aaataatttt attatttact actaaaaaaa taagtaaact aatagaagat
    23461 atggagagta aatattatgt atatttatga tttattagag caaggtataa gactaaacgc
    23521 atcagatata cacataacag ttggaacaaa tcctgtagct agagtaaaag gtggatttgt
    23581 aaaactaagt gaacaaatat tgacatcaga agtaacaatg caaatggcaa aagacatagc
    23641 gggggaatct atttttaaag taatagaaga acatggagaa gctgattttt cagcttctct
    23701 aaaaacagga gaaagattta gggtaaatgc atataggcaa aaaggaaatt acgcaatagc
    23761 aataaggact ataacagcag aaattccaac atttgaaaaa ttaggccttc cagagagtat
    23821 aaaatctttt actgaaaagc ataaaggatt agttttagtt acaggtccaa caggaagtgg
    23881 taaaagtaca acacttgcaa gtatgataaa tataataaat gaaaaacaac aaaaacatat
    23941 aattacactt gaagacccga tagaatatgt acatcatcat aaacaaagtt tagtaaatca
    24001 aagagaagta ggaactgata cagaaagttt tcattcagcc cttagagcta ttttgcgtca
    24061 ggacccagat gtaatactaa taggagagat gagagaccct gaaactgttt caatagcatt
    24121 aacagcagca gaaacaggtc atttagtatt ttcaacacta catacagtag gggcagctaa
    24181 gacaattgat agaatagtag acatgtttca accatcccaa cagcaacaaa taaaaacaca
    24241 gttatctaca gtttgtgaag gtgtggtatc acagcagtta cttcctacag cagatgggaa
    24301 aggcagaata gcagcaattg aattgatgtt tgctactcca gcaataaaaa acttgataag
    24361 ggaaggcaaa acatatcaaa ttccaaacat gatacaaact ggagttaaag taggtatgaa
    24421 aactatggac caagatttaa tggaacttta taaaaatgga aagattacaa aagatatggc
    24481 acttagtagg tgtactgacc aagagtttat gacaagaatg ataggtggag taaactacaa
    24541 tggatattat aatcgttaat ctatatgtat ttatagtggg tatagtgttt ggaagtttct
    24601 ttaacgtctg tatttttagg attccagaag gtatatctat ttctagacct ccttcccatt
    24661 gtatgagttg taatactaga ctaaagccaa tagacttaat accagtgcta agctatttga
    24721 tgagtggcag aaagtgtaga tattgttatg agaaaatttc tagtagatat gctatagtgg
    24781 aattaattac aggaatttta tttttaacag tatttatgtt atatggaata tctacatcaa
    24841 ctatttatta tttggttctt gtgtctttac tgattataat tacatttata gatattgacc
    24901 actttataat accagataaa atattgattt ttggagctat attttctatg atttttaacc
    24961 ttttatttaa agaaattcca gttaaggatt caattctagg tgtttttatt tgtggaggaa
    25021 gtgtctggat tatagttctt ttgattgaat ttgtaataaa aaaagagtgt atgggtggag
    25081 gagatatcaa actatttgca atgctaggcc tttacatggg tgttaaaaat ggtcttttga
    25141 cagccttact aagcgtttat gtaggagcag tctatggaat ctgtgtaata attataagta
    25201 gaataaaagg aaaggaatat aattctgtta taccatatgg accttttata tctattggag
    25261 cattaataac aatcttatgt ggaaagcaat tacttgaact ttatttttct atggttatat
    25321 agatgatagt caagttaaat aaagttttaa tttaaaaaaa tgaattaaag tatttaataa
    25381 ggacaatatt attattaacc ctattactaa agtgctacaa actatggagt atataataat
    25441 aatttcctta ttttttgcac atttgataaa taaatctcta tgtgtttttt gtgatagaga
    25501 aaagaaaaat ttatataata cgttttttat ttacataata actgtagttt tatctgtatt
    25561 aatatatcta aaatttggga ttagtgtaga atgtgtacaa tatatggttt taattccatt
    25621 tttaataatt atatccattg tagattacta cacaatatat gtatatgata taacaatagt
    25681 tagtggtata atagttcaag gtataatttt tttggccttt ttcaatatag ctcaatttga
    25741 ttatataaat catatatttg gactattgat aggctttatt ataccttata ttattgctaa
    25801 actaacaaaa ggattaggct atggagatgt aggtctctat agcttatgtt gctttgcatt
    25861 gggaaataat tatgggtttt atgtgatagt tttatcattt attttagcgt ttttgttttt
    25921 tattatttta tacataacta aaatatttaa agatgtagat ataagaaagc aaattccttt
    25981 tacacctttt atttccttgt caacaatttt aataatattt acagagtata atatatttaa
    26041 tttttattat aatttgttat acaaatttat ataacaggag gtaagaaatg tacgtagtag
    26101 tagggttagg taatccagga aagaagtatg agaagactag acataacgtt ggatttgatg
    26161 ttatagatat tcttgctaaa gaatacaata ttagtgttac taaaataaag cataaagctt
    26221 tgattggtga agggagagta ggtactgaaa aagtattatt agttaaacca caaacttata
    26281 tgaatttaag tggagaaaca ttaatagata tatataaata ttataaggtt gatttaagta
    26341 atatagtagt agtatatgat gatatagatt tagaagttgg aaagatacgt ataagaaaaa
    26401 aaggaagtgg aggaactcat aatggcatga aatctataac taagtgccta ggttcaaatg
    26461 actttccaag agtgagagta ggtgtatcta aaccagaagc tggtcaagac ttagcagact
    26521 ttgtactttc tagatttaga aaagaagaat ctgataacat aaatgaagca ttagaaaaag
    26581 cggcagatgc tattgactca ataataagag aaaatataga tatgtctatg aacaaatata
    26641 atggatagtg agagggagat ttaaatgaat gatgtttttt tatatccttt gcaaaattca
    26701 aaagagtata aagatataat aaactgtata aaaaatacaa aaggctcatt attggttaat
    26761 ggacttttac cagtacaaaa acctcatata tcgtattcta tatttaatga tttatcacga
    26821 caaatgattt ttataacgag tagtgattta gaagcaaaga aagtatacga agatttaagt
    26881 ttttatatgg aagataaagt tgaatattta ggttttcaag atatttattt ttatcacttg
    26941 gatgcaaagg acagaaacga agaagcaaag aaattaaaag tattgttaaa actagcaaat
    27001 aaagaaaaaa tcattttagt aacttctatt gaggcagttt taagaaaata cataccaaaa
    27061 caagttttgc tcgatagtgt atctcattat aaggtgggag atagcttaga cttagaaaag
    27121 ttaactgaga aattagtttc attgggatat gaaagagttt caaaaataga ggggtttggt
    27181 caatttagta taagaggtgg aattattgat gtattttcac ttgaatatac aaatccaatt
    27241 cgtatggaat tatttgatga tgagatagat tccatacgaa cttttgacgt atattcacaa
    27301 aaatcaattg ataaattgca acaattctct ataactccat ctagagaatt tatttatccc
    27361 gaaaaaacta cagatgcatt agttaaatta aaaaaagaaa caactaaaaa tactgatgaa
    27421 ggtacatttc aaaatataga ttatatatca agtaagactt attttgaagg tgcagagaac
    27481 tatatagatt atatttatcc agaagaaaac aaaagtatat ttacttattt agcagatgat
    27541 gcaatagtat ttattaatga tataacgaga cttaaagaaa gatgcgaaaa ttatataaat
    27601 gagtttagag aaaattataa attaaatctt gaaagaggtc tggctattaa aaaccaaggt
    27661 aaattgttat atcactatac agatttagag tacttagtaa aagacaagtc tgtggtatta
    27721 aactcgctat tgccaaagtc tataaataac ttttctataa aatctataat aaattttgaa
    27781 agtagagaag ttcctacatt taatggaaag gtagatttgc ttgtagaaga attaaatcgg
    27841 ctgaaatata atggatacaa aataatttta gcaacaaata ccttagaaag agcaaataaa
    27901 ttaggaaaag atttattgga caaaggctta gaaacgacca tttctaaaga tagagatatt
    27961 gaaattaaat cttcacaagt tataattgta cctgcacaca taaacagtgg ttttcagtat
    28021 aaatctataa agtttgttgt tataacagat aacgaaatga taggtgttta taaaagagcg
    28081 tctaagacta gcaataaaaa agttaaaaaa ggaaagaaaa tagaatcctt tttagattta
    28141 agtgttggag actatgtagt tcatgaaaat agtggtgtag gtagatacac agggattgaa
    28201 caaattacag tcaatgctat taaaaaagat tatatgaaaa tagtatatca aggtggagat
    28261 aatctatatg tgccaataga ccagatggac aaagtacaaa aatatattgg tgcagaagta
    28321 gaaaaagtta agttaaataa gcttggaaca aatgagtgga cgaaagcaaa agcaaaagtt
    28381 aaaaaagaaa ttgaagatat gacaaaagac ttgattgagc tttatgcaaa acgtgaaaaa
    28441 atacaagggt ataagttttc aaaagataca ccttggcagg cagagtttga atcgttgttt
    28501 ccatatcaag aaacagaaga ccaattaaag gctatagaag aaactaaaaa agatatggaa
    28561 tcaagtaagg taatggatag gctagtatgt ggagatgttg gttatggaaa aacagaggta
    28621 gctattagaa gtatttttaa agcttgtatg gaccaaaaac aagttgctgt attggttcca
    28681 acaacaatat tggcccaaca acattataat acattcaaag agaggtttga aaattatcct
    28741 ttgagagtag aagttttaag taggtttaaa acaccaaaac aacaaaaaca gattattgaa
    28801 gatgctaaaa aaggattagt agatatttta attggaacac atagaataat atccaaagat
    28861 ataaatttac caaacttagg tcttgtagtc attgatgaag aacaaagatt tggagtaaaa
    28921 cataaagaag ctttaaagaa aattaaatct acagtagatg tacttacact ttcagcaact
    28981 cctataccaa gaacattaca tatgtcttta agtggaataa gagacatgag tgttatagaa
    29041 gaacctccac aagaaagaca tcctgtaata acatatgtta cagagagtaa ggaaagcgta
    29101 attcaagatg agatagagag agaattaagt agaggtggac aggtattttt tgtatacaat
    29161 agagttgaac acattgaaga aatggcaagt atgattcaaa aactcgtacc agatgctaga
    29221 gtggctgttg cacatggaag aatgacttct aaatcattag aaaatataat cctaggattt
    29281 ttaaataaag attatgatgt actagtctgt actacaataa ttgaaactgg tatggacata
    29341 tcaaatgcaa atacaatgat tatatatgat gcagataaaa tgggattggc tcaactttac
    29401 caattgagag gtagggttgg aagaagttca agacaaggat atgcatatct tttgtatgaa
    29461 aaagataaaa ctttaagtga gatagcggag aagagattaa aagctatcag agaatttaca
    29521 gagtttggtt ctggttttaa aatagcaatg agagacctcg aaataagagg agcaggaaat
    29581 attttaggtt ctcaacaaca tggtcatatg gcagttatag gttatgattt gtacgttaaa
    29641 atgcttaatg atgcaattaa aaaagtcaaa ggtgaaccta ttgtggagga aattgatgtt
    29701 gaaatagatt taagtgtaaa tgcatacatt ccagataatt atatcaaaga tgagttgatt
    29761 aaaattgaaa tgtataagaa aatagcttct attgaaaaca aagaagacat gttggatata
    29821 caagaagaac ttgaggatag attctctgat atacctaaac cagtccagac acttcttaca
    29881 atagcttata taaaatcgtt atgtaagata ttaaaaatag aaaagataag acaattaaaa
    29941 gatgaaatat tattagttcc aataacaaag tatagaacta aacagaaaat aggttataat
    30001 atagttacag aattagaaga attattagaa aagatgtgca aggtaaaata acttgtaaat
    30061 ttaagaaggg atgttttaaa tttgaaaaaa gtaataacat tggtaatagc tatgattttg
    30121 gtagtatctg tgactgcttg cagttctagt aaaggagaaa ctgtggcaac agttgaaggt
    30181 acaaagatat catcagatga atttaaaaaa actatagctc tttataaaga ttctatggaa
    30241 caaacttatg gaaaagatat atgggataag gaagttgaaa agggtgtaaa atacaaggac
    30301 aagtttaaag atttaattct tgaccaactt ataactacag aagtaatata ttctcaagct
    30361 aaaaaagata acttacttcc taaaaaagag gatgttgaaa aaagttttaa agaattaaaa
    30421 gacgctatgg gtaaagatga aaaatataaa gaacaactta agaaattagg tatagatgat
    30481 gagtttttaa gagaccaaca agagaaagat ttagcaatgc aaaactatca aagtaacttt
    30541 gctaaaaaga ctaaaatatc agatgaagaa atgaaaaaat actatgatac acataaagat
    30601 gaatttaaaa aagatgaggt agaagcttct catattttat tgaaaacagt agatgataat
    30661 aacaagccac tttcagataa ggaaaaagct gaagctaaga aaaaggctga agaggctctt
    30721 aaagaagtta aatcaggtga ggattttgca aaagtagcaa aaaaatattc acaagatact
    30781 tcagcaagtg atggtggtaa attaggattc ttttcaaggg ggcaaatggt tgctgaattt
    30841 gaagatgctg ctttttctat gaaaaagggt gaagtatctg atttagttga aactcaatat
    30901 ggataccaca taattaaagt gacagacaga ataaatgagc aaacatcatt tgaagatgct
    30961 aaagaaacta taaaagacca actgttgaaa aataaatatc aagaacaaat agaaaaatta
    31021 actaaagaag ctaaggtaga aaaagatgaa aaagtaataa ataaaatcac aatctaagac
    31081 ttaaaaaaag agtaatatgc ttaaaaaagc tatatctgga taagatatag ctttttttgt
    31141 atatttttct taacaatggt aaaaataaac ttaaagtaat ttatggaggg attaaaaaat
    31201 atgaaagcaa caggtatagt tagaagaata gatgatcttg gaagggtagt tatcccaaaa
    31261 gagataagaa agacattaag aattagagaa ggagaccctt tagagatatt cacagcaaaa
    31321 gatggagaag tcatacttaa aaaatactct ccaataggtg aattaaatga attttcgcaa
    31381 gaatatgctg aaacattagc tgaagtacta ggatatggag tgttgataac agacttagat
    31441 tctataatag ctgtgtctaa gcttcctaaa aaagattata aagaaaaaag tataagtgat
    31501 gagttagaag caataataag tgaaagaaca gtaaaatatt caaaagataa aaacatttta
    31561 ccattattca aggaagacca aaaagaatat actagtcaag ttatcatgcc tataattagt
    31621 gcatctggtg actgtatagg ttcaattgta gttgtttcaa aggataaaga tacaattgca
    31681 gaaagtgaag aaaaatcttt aaaaattgct gctaactttt taggaaaaca agttcaataa
    31741 ttttatcata aagacctttt acatgaaggt cttttgaagt ttttgtgtaa aatattgaat
    31801 atagcattaa aaatatggta tattaaagtt tgttatataa acagtatgga ggttatgtta
    31861 tgaacaataa caaaaataaa gactcctttc taaaaggagc ccttatactt gggctagcag
    31921 gtgtaatcgt aaaaataatg ggtggatttt ttaggattcc ccttggaaac atgattggtt
    31981 cagaaggaat gggatactat caggctgctt atcctgtgta tactctattt ttaacattag
    32041 ctactgctgg atttccaaca gctgtggcaa aattggtttc ggaaaaagtt gcaattggaa
    32101 attttaaagg tgcaaataag atatttaagg tatcacatac agttttattc attaccggta
    32161 taatatcatt ttgtatatta ttttttggag ccgactacat agtaacaaac gtaatgaaaa
    32221 atccaggtgc gttatactct atgaaagcca ttgctccagc attactattt gttcctgcaa
    32281 tgtctgcata tagaggatat tttcaaggga gacaagatat gactaagatt gctgtgtctc
    32341 aagttgcaga gcaatttttt agagttgtat taggtcttac tttggcctac ttgcttatga
    32401 agagtctagg tcagactttt ggtgcagcag gtgctatatc aggggctaca ataggttcaa
    32461 tagcatctat gctttatttg gtgtttgctt atatgctagg gagaaaagag cgtagagcag
    32521 agatagatgc aagccaaaga tttaaagatg aaagagtgtc ttatattttt aaaaagcttt
    32581 taactgttgc cattcctata acaataggag catcagtaat gccacttgta aatatgatag
    32641 ataatgttat tgtaataaga agacttatgg aagcaggatt tacatacaaa gtggcaaatt
    32701 caatgtttgg tcaattaaca ggtatggcaa tggccacaat aaatttacca gcagtcataa
    32761 caactgcaat gagtatgagc ttagttccag caatttctaa agcatatgca ttaggaaata
    32821 agtcaaaagc tagaaaagat actaagagtg cagttaaggt aactttactt atagtattgc
    32881 cttgtgcgtt tggtatggct tctttagcaa taccaataat gggtctttta tttccacatg
    32941 aaccatcttc tgtaggaact attttgttta ctcttactcc atgtgtatta tttttaggat
    33001 taatacagac attgactggt ataattcagg gaatgggaaa accaatagta cctgtaattg
    33061 ctttatgtgt tggtatgcta tgtaaaattg taataagtta tactttaaca ggtataccag
    33121 atattaatgt actgggttca gcatttggta cagttacagc atactttgtg gcagccatga
    33181 ttaacttact atatgttaaa aaacacatga atgtaaactt ttctaaaaaa gaatttataa
    33241 taaagccatt tataacagtt atgacaatgt ttataatggt taagttgagt tatggtgctt
    33301 tagttggatt cttaggaaac agcatatcta caataattgc aatatgtgtt ggtggaattg
    33361 tatatgtagt agtaatactt ggaataggtg gaattaaaaa agaagagatt ctaactatgc
    33421 caaaaggtga taaattatat aaattgttaa aaaaagttaa acttataaga taatatagga
    33481 atatatatgc aaataaagga aatactatac aaatagtaaa tctccttatt tgtataataa
    33541 tataaaaata aagtgaaatt tgctaatagg agtgatgtta tgggtaagat aagtattgta
    33601 ggtcttggac caggagacta ttctcttata agccaaggtg tgttagaatc tttaataaat
    33661 agccctaaag ttttttttag aacagagaaa catccaataa ttgataaact taaagaaact
    33721 atacaataca cttctttaga ctacttttat gaaaaagagg aaaattttga taatgtttat
    33781 ttgaaaatat ctgaatttat catagaagaa tcagcaaagg gtgacctagt gtatgctgtt
    33841 cctggtcatc caagagttgc agagaagact gtaagtataa tagagagatt agccaatgaa
    33901 aagcacatag cagtagaaat attgccttct atgagctttg tagacgctat gtttaactat
    33961 ttggctatag acccatctga gggatttaaa ctggtagatg cttttgaaat agaaaattca
    34021 tatatagact tggatacaag catgataata actcaggtat atgataaatt tatagcatct
    34081 aatgtaaagt taaaacttat ggaacattat gactataatc aagaagtatg tatagtaaat
    34141 ggagcaggtg tcaaagattt agaaagtaaa aagtttataa agctttgtga attagacaga
    34201 aatgaaaact tttttgacta tttaactagc ttatatatac ccaaaagttc taaaaagatg
    34261 tataatacag tacatgactt agaaataata gttaatacac ttagaagtcc atctggatgt
    34321 gaatgggata aaaaacaaac acatcaatca ttaaaaaatt ctgttataga ggaagcatat
    34381 gaactttgta atgcaattga taataatgat atagatgaaa tggtagaaga gttaggtgat
    34441 gttcttttac aggttatatt ccattgtcag ataggaaatg aggagggcta ctttgattta
    34501 aaagaagtag taaatggcat atgtaaaaaa ttaattcata gacatcctca tatatttagt
    34561 ggtgcaaaat tagatatgtc taagtttgag aaaacttggg aagaattaaa gaaagaagag
    34621 aaaggcgaaa gtactataac agatggcctt agaaggatac caaaacattt accagctctt
    34681 atgaaggcta gtaaagttca acataaagca gcacttgtcg gctttgattg ggacaatgta
    34741 gaagatgtat ttaaaaaaat tgaagaagaa tatcaggaat tacttgacga acacagggtc
    34801 gggaatataa aatacataaa ggaagagcta ggcgatttat tattttcgat agtaaatcta
    34861 gctagatttt tagacataga ctcaggagaa gccttgaact gtactacaga aaaattcata
    34921 aatagatttg actttataga aaaaagtgca atgaacttga ataaaaaatt agaagacatg
    34981 acacttgaag agatggatga gttttggaat caggctaaaa aacaataaaa ttgtggaagg
    35041 aaatttgtca aaaatctaga acttttatca caaagataga agtctatatt ataaacattt
    35101 ttaggaggta aatatcgtga ataaagctga attagtatca aagatggcag aaaaaagtgg
    35161 attaacaaag aaggaagcag aagctgcgtt aaacgcattt atgagttctg ttcaagatgc
    35221 actagtaaat aatgaaaaag ttcaattagt tggatttgga acatttgaga caagagaaag
    35281 agctgctaga caaggaagaa atccaagaga tccagagcaa gttatagata taccagcttc
    35341 taaagcacca gttttcaaag ctggaaaagg attaaaggat ataataaatg gataattaga
    35401 aatcttgtga aaaagtgtgg ataaatttgc cacacttttt ctatagaacc aaggaaggtg
    35461 ataattatga gattagataa atttttaaaa gtatctagga ttataaaaag aagaactgtt
    35521 gctaaagatg catgtgataa agggattgtc actataaatg gaaaagttgc taaatcttct
    35581 tcagaggtga atattggaga tacattagaa atacaatttg gagagaagaa gatgaagttt
    35641 aagataaatg aagtaaaaga gcatgtatta aaaaatgatg caaaagaaat gtacgaaatt
    35701 ttagaataaa attaatagtc ctctagtata ttaatagtag tattattgaa atatttactg
    35761 gggggatatt atggaacaga acataacttt gaaagataga tcaaagctgg ttatttcggg
    35821 agtcgagcac atatactctt ttaatgacaa aagagtagag ctcaaaactt ctgtaggcga
    35881 gatggtaatt gaaggcgaaa acctagatat gagcaagcta agtatagacg aaaatataat
    35941 tagcatagat ggaactataa attctatggt atatgcaaag cctaaaaagc cacaagagag
    36001 tttcttgaaa aaggtattta gatagatgat accttttact caagatgtaa gcatttttta
    36061 tgctactatt tatggtggaa tactcatagg ggtgttgttt gatttttata gagggctaag
    36121 agggaatttt aaattcatca attattttgc tataatcttt gatgtattgt tttggtttct
    36181 agctacggtt ataatattcg taacaataaa ccttacagaa ttttttgatt taagatatta
    36241 tcattttgtg gctttgttta ttggttttat tttatactat aacaccataa gtaaaattgt
    36301 tttatcaatt atcaataaaa taattcgttt tgtgagaaat tcttttaaaa aagttacaca
    36361 ttacatagtc agttttttaa ataatttgta ttacgttata atctattcac tacacctact
    36421 atttgatatt atattttata taccaaacat atttatagca acaagaaaaa gtataaaaag
    36481 aaaatcaaat aaaaaattaa agaacaagaa aaaatcaaaa cctaagaaga aaaaaaataa
    36541 aactaaaaaa agggtgtagg aaaaatgaac ttaagaaaaa aattttcagg acagattatt
    36601 gttatcagtt tatttttagg aatatcaatt tttagtatga tgacaggatt tgtatttgag
    36661 tatacaaaag ctaaggaata taaaaaagaa atagcaagtc taaataaaca attaaaaaag
    36721 actgaaatac aaataaacag cttaaagaaa gatgaaaaat catatgaagg agacttagag
    36781 gatatagcaa gaaaaagatt gaatatggta aaaccaaatg aaacagttta tgtagacata
    36841 aatcgttgat taaaacttgc taataagttt acagatgtat tgattttaaa atttaagatt
    36901 taataaaaaa gtcattcagt taaggatggc tttttgtttt tattgaggag ggaatttcat
    36961 aatgaaagta ggagctatag atattgggac aaactcaatg agattactga tagcagatta
    37021 tagagatggg aattttataa acagaaaaaa atttgttaat acaactagaa tcggttatgg
    37081 tgtggatagt aaaggatata taaaagaaga tgctatcaaa agaaatataa aagcattaaa
    37141 agaattttct gataagtgta atgatgaaaa gtgtgaaaaa atttattgta tggggacatc
    37201 tgctcttaga gacagtaaaa atggagatta ctttgttaaa ttagctaaag aagaaactgg
    37261 aatagatgtt gatatagtta cgggaataga agaatcaaat ttgggcttta agggtgtttt
    37321 agaaggaata caaggtgatg atactattat tgttatagat attggaggag gttcaacaga
    37381 atttattgta ggtgattcag aaggaattaa atttaataaa agtgagaatg taggtgctct
    37441 taggatgaca gagaggtttt tgaagcacga tattattgaa gatgaagagt ttgaagctat
    37501 gagttcattt atatatgatg agattgacaa aacgttaaat catataaaag acagaggatt
    37561 aaaaactctt ataggtatag gaggtacgat tacatcatta tcagcaatga atcaagaatt
    37621 agagatttac tctatggaaa aaatacataa tagtaaaata cacaaaaaag aaatagaaga
    37681 aattctacaa aaattaataa agatgacatt aagtgataaa aagaacttaa aagggctaca
    37741 agaaaaacgt gcggatatta taactgctgg tgtaaaaata ttgaatataa taatggaaaa
    37801 attagaaata gaagaaataa ccgtaagtga atacgataat ttggaaggct taatatgtca
    37861 aaagtcaaaa aaaatgtatt aatatttttt gatttttaaa accgattatg acaaaaaaaa
    37921 ttactcctct ttgttacaat tcttaatata aacaaaagag gaggtttttt tatgcaaaga
    37981 agtgtagcag ttgtgaagaa taagactgta aaaccaaatg tcataaaatt aataagcgga
    38041 tatattgatt ttactacggc ggcattcgct gtgatgggat ttttacttag taggtctatt
    38101 atagtagact ctgtagctcc tttaggaatt gcctttttta tatgtgcatc aaagattgat
    38161 aagtacaaga tacctgtatt catatcaaca ttattaggaa tattgttatc atctaatgat
    38221 tttggaaatg tagcaaaata tgctgtgtgc cttacaatat ttatggctat gaataaaaaa
    38281 ttaaaattat cagattcaat tgctaaggtt gcatttatag gtgcaataat actacttcca
    38341 atctccatgg gacaagcttt cttttcaaat aggtatgtat atggtcttct tatagcagga
    38401 gtagaagcaa taataatgtt tatatctata tatgtgtttt cttatgggat aggtttaatg
    38461 gccaatgtca acaataggat gtctgtacgg acagaagaag ctatatcaat aagtttactg
    38521 ctaaccttta gcataatggg aataggagat atatctctgt ttggaatatc aataaggacc
    38581 attttatcga ccgtattgat attgctgacc tcaatagtag gtggagctac catgggagca
    38641 tctagtggtg taattattgg tgtagcatcc attttaaata atatgacatc agctgtttat
    38701 atggggatat attctttttc aggattaata agtggagcat ttaataaaat aaataaatat
    38761 ttctgtatac taggatatat tctaagttgg acaataatat acctatatac ttctggtatt
    38821 acttctaata tgatgcaatt aagagatata ctattaggtt gtttgatagt actagtattg
    38881 ccagagagat tatttaataa gattgaaaaa ttaataaaaa gtaatgtggc ttcaaatgaa
    38941 atcgtatacg attatattat gagaagcaaa aacctaacta atagcagatt gaacagtatt
    39001 tataaaactt atgatgacct tgcagataca tttgataaga taagagaaaa agacaaagtg
    39061 ctagaccaaa gagatatagc taatgtaata gatatgattc ataatgatga atgtaaaagt
    39121 tgtagtatga gaagaatgtg ctgggagtca agatttaatc atacatacac tatgatatat
    39181 aacattcttg aaaaaattga ggagaaaggg gagttaagtc taaacgatat acctaaaaat
    39241 tttagaaagg aatgtatgaa accagagtcg atagtgaaaa tatcaaatca ctattacaaa
    39301 atgtttgtat tagattatga ttggagtgtg aagttttcag aaagtagaaa gttaatagca
    39361 aatcagataa gaagtatttc aaaatccatt aaatccttat ctcaagattt agaaggtgac
    39421 ataatgctag atatagaaaa ggaaaaaaat atatatgaac agttagaaag atatgatata
    39481 acagttgata aagtaagtta cttaacaaaa agtaacaatg agtttgaaat atcaatagaa
    39541 aagaaaactt gtcatgatgg atgtatgtgt gaagataaaa ttgtaaatat aatttcagac
    39601 ctagtaggtg aaaatatgtc tgttagaaaa ataggatgtc actgtttagg aggtaaatgc
    39661 aaggcaacat tcgttaaatc tcaaaaatat aaggctgtta cagaagtttc agcaatgtca
    39721 agggatgggc atatcctttg tggagacaat tacacataca tggagataaa tgatggtaaa
    39781 tacatgatgg ctataagtga tggtatggga aagggtaaaa aagcatatga agaatcttct
    39841 gccactattg atatactgga aaaaatgata gatgcaaaaa ttaaagatga gatagtcatt
    39901 gatactataa ataatatgtt gttgttaaaa tcttcagaag agatgttttc aactctggat
    39961 ttgggaattt tagatttaaa acgaggttgt ttagaaacta taaagatggg agcgtgttct
    40021 acttatataa aaagagagga tggagaagtt gacttaatat catcatcatc attacctgtg
    40081 ggtatattat cagatgtaaa aattgataga aaaaatgtaa aagttaagga aggagactat
    40141 gttataatgg tttctgatgg aattgtagat gcaggaagaa ataacaattt aggagacaat
    40201 tggttaatat acttcttaaa gaatatagag actacaaatc caaaggaaat ttcaaattta
    40261 atattggata gagcattaga gttacaagcg ttacaaatcg aagatgacat gactgtttta
    40321 gttacgaaaa tatgtacaaa ataaggttta caagattatg tgtataagat gtggataact
    40381 tgtgtataat atgttaatag acttttgaaa aaagtatagt tattaacaaa aataacatat
    40441 aaatcaacaa aatttgttga taagtatggc agaaatgtta ataaaatgta aaaaaagaaa
    40501 aaacaaaagc tattatcaaa agagattcat gttgaaataa ctaaatttct aagtaaagaa
    40561 aacctgtgaa tatgttgatt attatgtgga taactattaa ttcgatattt atttatatag
    40621 tatgaataat tgaataatat atctaaaaaa agccatccta ttattaagta aatttattga
    40681 aaggatggct ttttatgaat agaaaattgt gcaaagcaat aataccttta tttatttcta
    40741 ttttttgcat gttaataata agtataaaag ctgaccaagg atatcaaaag aataatgaca
    40801 gagcaattgc tgtcatgaat aataatgata ctgaagatga tgctgaaaaa aaacaagaag
    40861 tagactatga aaaaacatgt tggaatttgc aatctttgtt caaaagtgat gaccaatgga
    40921 aaaaagaact taagagtttt gataaagata ctaaagagtt gaaaaattac ataggtaaag
    40981 taacaaaatc gccaacacat ttgtcttttg ccttaaacat taaggaaaaa cttgatataa
    41041 gactaaataa actttctgcc tatgtaaaat taaaacaaga tactaataaa aattcatata
    41101 agtacttaga tatgagtgat agtatgagta aatcatatgg gaattatatg gggatttgtt
    41161 cagatttaga attagaaata ctaaaattat caaacaaaga ttacaagaaa attatttcaa
    41221 ataaaaatat aaataggaaa tatggtgcat atttaagaga tataagaagg aataaagtac
    41281 attatttgga tgataaatca gaagatattc taagcaaagt atcttcaata agtagtcttc
    41341 caagccaagt atatgaatta tttagaaata tggatagaaa aactaatttg acaccagcgc
    41401 aatatgcaag tgaattagag tcagtagata gagaaaatag aaaaaaagct tatcaagatg
    41461 aacttataac ttacaatgat aatataaata caattgcagg tttaattact ggtcaagtta
    41521 aaaagaatat tttttattct caggaaagag gttatgagtc ttctttagaa atgtacttgg
    41581 aatctgataa tgtagatgaa aaagtataca ataatttgat aaggactgta aatagtaata
    41641 tggacagtct acacaaatat atagatttaa gaaaaaaagt attaaaatta gataaggttc
    41701 attcatatga tatgtttgtg cctatggtta aggctgttga taataatatt aattatgaaa
    41761 aggcacaaag tatagtatat tcagcgttgt cacctttagg aaaagaatac aatgatgtag
    41821 tatataaagc tttcaatgaa aaatggatag atgtttattc acatgaaaat aaagtaagtg
    41881 gtggatactg cttatctgtt tatgaaaatc atccatatat acttttaaat tataataatt
    41941 cattaggttc agtttcgact ttatcacatg agcttggaca tgctgtatat gaatacttaa
    42001 gttctaagaa tcagaattat tttaattcaa atccgtctat atttactcac gaagttgcat
    42061 ctacaacgaa tgaagcatta ctttacgaga tgcttataaa agaagctaaa ggcaatgatg
    42121 aaaaagctta ttatataaca cagtatttag acttgataaa agatactctt tatacacaaa
    42181 ctatgtatgc tgaatttgaa aaaactatac acgagttagt agaaaaagga aaaagtgtaa
    42241 acgcattagt attaaatgat gtttggggac aacttcttaa aaaatattat ggacaaagct
    42301 atgaattaga ccaattatct aaggtcggat gggctagaat cccacatttt tataatagtt
    42361 tctatgtcta taaatatgct acaggttgta gtgcaggagt aagttttgcc caagacatcc
    42421 taaagaatgg ttcagataac tatatgagct ttcttaaaaa gggaagttca gattatccaa
    42481 taaacttgtt aaaatcaagt ggcataaatt taactaatac aaaacccata gaaaatacaa
    42541 tcaaaaaatt tgatagttta gtagcagaac ttgaaaagct attataaaca ttattgtaat
    42601 ccacatggta aatatatcga attaatgata aaatatacta aaagtttaaa gtcctatcaa
    42661 gataggactt tttatttatt aattagtggg agatgattct atgcaagtta aagttaaaaa
    42721 ggctgtaata ccagcagcag gtttaggaac tagatttttg ccagctacaa aggcacaacc
    42781 aaaagagatg ttaccaatag ttgataagcc aactctacaa tacataatag aagaagctgt
    42841 ggcctcagga atagaggaaa tactaataat aacaggtaga aataaaaaat ctattgaaga
    42901 ccactttgat aaatctgtag aattggaact tgatttagag aaaaaaggta agaaagaatt
    42961 gttggaggta gtacaaaaca tatctaacat gattaacata cattatataa gacaaaaaga
    43021 gccaaaagga ttgggagatg caatttattg tgcgaggcat tttataggtg atgagccgtt
    43081 tgcagtaatg ttaggggatg atattgtaga taatgatgta ccttgtctaa aacaactaac
    43141 agatgcatat gaggaatata gaactacaat attgggtgtt caaaaggtaa atcaagaaga
    43201 tactaataaa tatgggataa tagaagcaaa aaatatagaa ggtagagttt acaaggtaaa
    43261 agacatggta gaaaagccag agtcaggaaa agctccttca aatattgcta ttttaggtag
    43321 atatataata actcctgaaa tctttgatat tcttaaggat ttaccaccag gtaaaggtgg
    43381 agaggtacaa cttacagatg ctttaaaaat attatctaag aaagaagcta tgtatgcata
    43441 caattttgaa ggcaagagat atgatgttgg ggacaagcta ggatttttag aagctactgt
    43501 tgactttgct ctaaagaaag aagaccttaa ggaagatttt ataaaatatt tgaaacatgt
    43561 atgtagtgaa tttgataaaa ataacaatgt gttatacaat aatacagatg aaaaaataga
    43621 tttaattgaa gtcaatgaag ctagagaact tgaaattcaa aaataattaa tgaaaggtga
    43681 tgtatggtgg caaaatcaag tcagaaaaga attaatagaa taatagctgt tgggattgtg
    43741 ggaatcctcg taataacgag tataacagct gttttgagta tgattgcaac tatgtaataa
    43801 gataaatgac agcagttgat atacataaat ataaaaacca tatataactt gatgtagtca
    43861 aaaacttaca atattttagt tatatatggt ttttagaatt tataaatgta aaaaaaaatt
    43921 tttataatag ttgcatattt taaaggaaaa tattattata agatataaat atagtgataa
    43981 atttataaaa acgtgctaag attggacttg aaatacagta atatagtagg taataatgtt
    44041 ttaaatttta tttattgacc ataatatata aataatgata ttattagact taactgttta
    44101 aataaaactt ttctttttca tgccttcaat ttccaaaata agtatgtata taaatgaata
    44161 tatagatgag gaggattttt ttgattttag atgataaagt attagaagga ttaggtagta
    44221 agaccttagt tgagctacga gagatagcta aagaattaaa aataaaatca attactacat
    44281 ataagaaaaa tgagttaatt gagattataa attccaaaag taaaaatgag gaaaaagtaa
    44341 acgaagagac taaagaaaaa aatgaagata ttaaagaaga cataaagaat agaaaagaaa
    44401 atatagagcg tgaaagtata gaaaaagaag ttgaatataa gagccctacc aaatcataca
    44461 acaagaaaga aatagatacc caaaatattt cccaaaacca agaaaataga aatcaaagaa
    44521 atgactataa taagacaacg agtgtaaatg atagtaataa gtcatcaaat atatctaata
    44581 gaatggtcag aaataataat aaaaactatt atatgcctaa acaagttgat gaatctaaga
    44641 tagtagatga atttaacaca tcaaaagagg atgaagtagt aggtgtactt gaaattctgc
    44701 cagatggatt tggtttttta agaggttcaa attatttgtc tactgaagga gatgtttatg
    44761 tttcaccatc tcaaataaga agatttaata tgaaaacagg agacaagatt aagggaataa
    44821 ctagacatcc aaaaagtgga gagaaattta gagcactttt atatgttcaa aaaataaatg
    44881 atgaaaaccc tgacacagct atacaaagaa atgcgtttga gacattaaca ccaatatttc
    44941 cagaagaacg acttacactg gaaactaata gaaatgaaat agctacaaga attatagatt
    45001 taatttcacc tataggaaag ggacaaagag gattgatagt agcacctcca aaggctggga
    45061 aaacagttct tttaaaaagc gttgcaaata gcatagctaa aaatcatcca aatgttgaac
    45121 ttatagtact ccttatagat gaaagaccag aggaagttac tgatatgaaa gaatctattg
    45181 aaggagacgt tatatattca acttttgacc aagtatcaag tcatcatgtc aaggttgcag
    45241 agatggtgtt aaatagagct caaagattag ttgaacatgg taaagatgta gtaattttac
    45301 tagatagtat aactagattg gcaagggcat ataaccttac aatatctcct acaggtagaa
    45361 ctttatctgg aggtattgac cctggtgcac ttcatggacc taaaaagttc tttggtgcag
    45421 caaggaatat tagacagggt ggttctttga caatattagg aacagcattg gttgaaactg
    45481 gttctaggat ggatgatgta atatttgagg aatttaaagg tacaggaaat atggagctac
    45541 atttagatag aaaattagca gaaaaaagaa tattccctgc aatagatatt tacaagtctg
    45601 gtacaagaag agatgattta ttgcttgatg atgaagaaaa gactgctctt tggagattaa
    45661 gaagagaaat gagtaataat tcagtaatgg aaattacaga taaagtcatt gaacttataa
    45721 agagaactaa ggacaataaa gagtttgtaa aatcaataaa gaatttgtaa aatcaataat
    45781 acaaatgcaa atatggatta tataaactaa tagaaaaatc acttgaatgg cataataagt
    45841 tgtgctataa taatgtagtt gataatgtaa aaagaaagtt aatataatta atttttttaa
    45901 ataaagagaa gaggtgacta taatgcaaaa ggaaatacaa ccaaaataca atccagttga
    45961 agtgcgttgt gcttgtggga acacatttgt tgctggttca actaaggacg aaataaaagt
    46021 tgaaatatgt tcagagtgcc atccattcta tacaggaaaa caaaagaaca tagaaaaagg
    46081 tggaagaatc gacaaattca agaaaagatt caaaatggac taatttttat aaaagtggag
    46141 ttggaggaag ccaactccta ttttattaca aaaaaacatt catattctac tttaaattgt
    46201 ctttttgtat tataataata aatatgtaaa attgagtatt ctaaatacaa taaaatgagg
    46261 tgagtggatg gcaaaacaag ctgtaggtgg gcaagctgtt attgaaggtg taatgatgca
    46321 gtcaaaagac aaaagagcgg tagctgttag aaaaagtgac ggagaaatag cactaaaaga
    46381 agatagaata aaaagttggg taagagataa gaatatagat aagatacctt ttgttagagg
    46441 ttcttttgtt atgatagata caatgattca agggataaaa agtttaaact tttcttcaga
    46501 gttttttatg gaagaagctg aagaggataa atttgattta tttataaaga aaatttttaa
    46561 agataaggca aatgatataa ttataatatt ttcattggtc atagcaatgt tactttctgc
    46621 tggattattc atttttatac ctacattggt aggaggagct ttttctaaag taatgcctaa
    46681 tgattttatg cttaatttaa tagaaggaat cataaggata gctatattat ttgcatatat
    46741 agtattgatt tcacgaagta aggatataga aagagtcttt caatatcatg gagcagaaca
    46801 taaatctata tattgctatg aaaatgattt agaacttaca gtagagaatg ctagaaaatt
    46861 taaaagatta catcctagat gtggtacaaa tttcctattt atagttatgg ctgtatcaat
    46921 aatattgttt gcattttttg gttggccaaa tccaattctt agaatattta tgagaattat
    46981 atgtgtgcct atagtagcag gtttatctta tgaggtaatt agagttcttg ggaaatatga
    47041 taatggattt actaaaatta ttgcatatcc aggaatgatg ttgcaatatt ttacaacaaa
    47101 agagcctgat gatgagcaat tagaggttgc tttggaagca ttgaaagcag tagtagatta
    47161 gagaaaagga tatttgttat gacgataaaa gatataatta ttaaatattc agataaacta
    47221 aaagatataa gtgatactcc aagattagat acagagttat tgttacaaaa aacattgggt
    47281 gttgatagat tatatattca cttaaatctg aataaagagc taactgaaga acaaaaaaca
    47341 aaatttatgg gttttgcaga agaaagatta aatggaagac caatagcata tatagttgaa
    47401 aatagggagt ttatgggatt agattttttt gttaaggaag gcgtattgat tccaagacca
    47461 gatacagaaa ctttagtaga agaaataatt gaaatatgta gagaaaaaaa agatgtaagt
    47521 atattagata taggaactgg ttctggagct attacaataa gtttagcaaa gtacattgaa
    47581 aattctaaaa tcatgtcttt tgatatatca gagacagcat tagaaatagc taaaaaaaat
    47641 gctattataa atgaagttgg tgaaaaaata aagtatatta attctgattt atttacagca
    47701 ataagtgata gtaacataaa attcgatatc atagtttcaa atccaccata tattaaaaaa
    47761 caagatatag aaactctgca caaacaagtt aaagattatg aaccatataa tgctcttgaa
    47821 ggtggagaag atggattaga cttttataga agaataacag aacaaggtaa gaaatattta
    47881 aataagtgtg gaatattagc atatgaagtt ggtcataatc aagctgaaga tgtaataaat
    47941 attatgaaaa gtaatggata taaaaaaata tacacaaaga aggatattca aggcattgat
    48001 agagttgtta taggttacaa tatatgatat aatcaacaat tgatatgtta catttaatcg
    48061 aggtgaaagt atgttaaaaa aattagaggt attggaagat acatataaag acttaagtga
    48121 aaaaataggt gatccggatg taataaatga tcaaaaagtt tggcagaaat atataaaaga
    48181 acatgctgac ttggaaccta ttgttatgaa atacagagag tataaaagcg ttttagatag
    48241 tataaaagaa tcaaaagaaa ttttacaaga agagtcagat gaagaattaa gagaattagc
    48301 taaaatggag ttagctgaga tggaagaaaa agtagcacct ctggaggaag aaataaaaat
    48361 actattattg cctaaagacc ctaatgatga taagaacgtt atagttgaaa ttagaggtgg
    48421 agctggagga gatgaagcag ctctatttgc gggtgattta tttagaatgt actcaaggta
    48481 tgctgaaaga agaagatgga aaatagaatt attgagtgca agtgatactg gagttggtgg
    48541 atataaagaa gtatctttta tgataaaagg taaaggtgct tattcaagat taaagtatga
    48601 atctggagtt catagagttc aaagaatacc atcaactgaa tctggtggaa gaatacacac
    48661 atcaacttct actgttgcag tattaccaga agttgaagat gtagaggttg aaataaatcc
    48721 aaatgattta agaatagatg ttttccgttc ttcgggtaat ggaggacaaa gtgtcaatac
    48781 tactgactct gctgttaggg taacacatat acctacaggt gaagtagtat cttgtcaaga
    48841 tggaaaatca caattaaaaa ataaagagca agctttaaaa atattaaaag ctagacttta
    48901 cgataaagca ttggcagaac aacacaaaga tatagcagca gaaagaaaaa gccaagtcgg
    48961 aacaggagac aggtcagaaa gaataagaac atataatttc cctcaaggta gaataagtga
    49021 ccatagaata aatttaactt tatataagtt agatgcattt ttagatggag atatagatga
    49081 aatgatagat gctttaatta ctgttgacca aacagaaaaa atgactgcaa tataagtaat
    49141 gttttaaaga agctgtttct tcctagaaat tattttctat tttgaaacag ctattttgca
    49201 ttttaggtca tgttatcata ttctattgta tataattgtt atatacaagt ataggagggt
    49261 tatatgatac taaaagtaac aataattggg cttttggcag gtgttatagg gacaggatta
    49321 ggaggagtaa tatcagccat ttttaaaaga gaggtcgata aatatctaag tttttttatg
    49381 ggattatctg gtggaataat gttggctgta gtggtgtttg atttaatgaa agaatccatg
    49441 gacaaaatgg ggataatcaa tacagttata tttacgtttg ttggagctct cataactatg
    49501 tacataaaaa caaaattaga tgtgtctgga aatatggcat ctggatatct tatattcata
    49561 agtatattac tacataattt accagaagga ctagccattg gttcttcttt tatgtcaact
    49621 gaaagtttag gtattacact tgctatagtt ataggtctac ataatatacc agaagggtta
    49681 gctatggcat taggcttagt ttgcaataaa atgaaactaa gtaaagttat tttgtttact
    49741 gtaatagctg gattacctat gggtctagga agttttttag gtgtttattt tggaggagta
    49801 tttacttctc ttataggagt atttcttgct acagcaggag gtactatgat gtatgttgta
    49861 ttggaagaaa tttttccaca ttcaaaaagt gtgtattgta taattggttt cttgttaggt
    49921 acaatgattg taaattatat ataaaattaa aatgaaagca ggcatacaaa agatgaaaac
    49981 tattataagt aatattgata taaataatat tgattatgag gaaataaaaa tacaagcaaa
    50041 acttcttaga gaaggtaaaa cagttatttt tccaactgag acagtatatg ggttgggtgc
    50101 aaatgcatta gatgaaaatg cagtaaagaa gatttatgaa gctaaaggaa gacctagtga
    50161 caatccattg atagtccata tatatgaaaa agaagaagta tatgacttag ccaaagatat
    50221 aagtgataaa gctaaacttg ttatagaaaa gctttggcca ggacctataa caattatatt
    50281 aaataaaaaa gatataattc catacaaaac tagtggaggg cttgatacag ttgcaataag
    50341 gatgccttct aatgttattg ccagggcaat aataaaagaa gctggtatac ctattgcagc
    50401 tccttctgca aatatctcag gtcgcccatc tccaacaaaa gcaaaacatg tatatgaaga
    50461 gatgaatgga agagtggatg gaatagtttt aggaggagac agtaattttg gattggagtc
    50521 aactgtattg gatttaacag aagagactcc tatgatactg agaccaggaa gtataactaa
    50581 agaagtttta gaggaacttc ttggagaagt aaagcttgac ccatcattaa gtaaaaaaga
    50641 agataatcaa aaagctaaag ctcctggaat gaaatataaa cattattctc caaatgcaga
    50701 tgtatatata atatctggaa aaagagaaaa tgtagcaaca aagattaatg atatgataaa
    50761 atctaataga gacaaaggtc taaagacagg tgtaatgtgt atcagtagaa accgagagtt
    50821 ttatgatgga gaagtaatcg atttaggaaa ctctcttgaa gaagttgcaa gtaatctgtt
    50881 tgatgctctt atagaaatgg acaaaaaggg cgttgatgtc atatattcag aagagttccc
    50941 taagactgga gttggacaag caattatgaa tagactactg aagtcagcag gatacaaagt
    51001 gataaaggct taagagggag ttgtaaaata tgaatatact tattgtgtgt acaggtaata
    51061 cttgtagaag ccctatggct gaggctatac taaggaaagc aattaaagaa tctggaagaa
    51121 gtattgaaga atattcgata tcatcagcag gtatatctac agcaaatgga atgggtgctt
    51181 ctgaaaattc tatagaagta ctaaaagaaa tcggtataga cttatcaaat catagaagta
    51241 aagtaattac taaaaaatta attgatgaat cagatataat actaacaatg actaaatctc
    51301 ataaagaaat tttagtacaa gcagttccaa aatgcaaaga aaaagtgtat acttttaaag
    51361 ggtttgcaaa taaaaatgag gaagatatat ctgacccatt tggtggaaat ttggatattt
    51421 atagaagcac tatgagagaa ataatgtatt ctgtcaacga aatagtaaaa aaaatatgaa
    51481 acggaggata aacaaatgaa gataggatta ggctgtgacc atggaggata taatttaaaa
    51541 aaagaaataa taagctattt agaaagtaaa ggtatagaat gtgtagatta tggaactaat
    51601 aatgctacag attctgttga ttatcctgtt tatggagaaa tagttgcaaa ttcagttata
    51661 aataaagaag tagattatgg aatattatgt tgtggtacag ggataggaat atctttagct
    51721 gctaataagg ttcctgggat aagatgtgca gttgtatcag atgtattttc tgcaaaaatg
    51781 tcaaaagcac ataatgatgc aaatatgtta tcattaggcg aaagagtttt aggaaaaggt
    51841 ctagcacttg agatagttga agcatggatt aatacggatt ttgaaggtga tagacatgct
    51901 agaagagtaa acatgattaa atcaatagaa gaaaaacata ataaataata aaaattacag
    51961 ttttatcagg aggaattatc aaaatgagca aagtggtaga gacaaaccat ccattaatac
    52021 aacataaatt aactttaatg agagataaaa atactggttc taaagacttt agagagcttt
    52081 taactgaaat tgcaatgtta atggggtatg aaataacaaa agatatacca ttaaaagatg
    52141 tagaaataga aactcctatt caaaaaacat catctaaagt agttgcaggt aaaaaattag
    52201 caataattcc aattttaaga gcaggtcttg gaatggtaga tggattagta agcttaatgc
    52261 cagcagcaaa agttggacat gtaggactat atagagaccc agaaacatta aaaccagttg
    52321 aatattattg caagcttcct caagacatag gagaaagaga tataatagta gttgacccta
    52381 tgcttgcaac agggggttct gctgtagcag ctatagattt acttaagtct aaaggagcta
    52441 aaagtataaa attagctaat ttagtagctg caccagaagg tatagcagaa gtgcaaaaat
    52501 atcatgatga tgttgatata tatgttgcat cagttgacga aagattaaat gaacatggat
    52561 atataattcc tggtcttggt gatgctggag atagattgtt tggaactaaa taaaaataaa
    52621 taactataaa tatttattag ggactttctt atatagtgta tataatttta tgtaagaagg
    52681 ttctttttta tatatttttt ctcaaaatag gaattatgaa ttgataaatt tctcgaattg
    52741 agaataaaaa gtttttacca attaaaaaag attgcttatt ttacaaggtt ttatagaata
    52801 gtagtctatt ttaatgtggc atgttagttg cttacgatta agattatagg tattaagtgt
    52861 attagttaaa ttgagaattg aatattaata atattattgg gagtggtaat acatgaagaa
    52921 ttgtatagca gtaagagggg ctggagattt agcaacagca gttattcata aattacacaa
    52981 cagtggtttt aaagtagttg cgttagaaat agaagagcca ttagctataa gaaggcaaat
    53041 ttctttttgt gaggctgttt atgaaaaaaa agtaactgtt gaaggtgtag aatgtatatt
    53101 ttgcaatact gaaaaagaaa tagaagaagt tttatctcaa cataaagtag ccttgattat
    53161 agacccaaaa ggagaaaaat tatctaagtt aaatccaatc gttttggttg atggaatact
    53221 cgctaagaaa aacttaggta caactaaaga tatggcacca cttacaatag ctttaggtcc
    53281 tggattttgt gcaggagaag atgtagatgt tgttatagag acaatgagag gtcataatct
    53341 aggaagaata ataactaatg gtagagcaga aaaaaataca ggagtaccag gagcaataaa
    53401 aggtttttct aaagagagag ttatgcattc acagtatgat ggtataatgg aaaatgtatg
    53461 taagataggt gatatagttg aaaagggaga cctactttgt tatattaata attatgaaaa
    53521 aaaacatgaa gttagagcaa caatatctgg agtccttaga ggtttattaa aggatggagc
    53581 aaaggtgaac aaaaatttaa aaatactaga catagaccct agaaaagaag aagttaagaa
    53641 tgcttttaca atatcagata aagctagatg tatagctgga ggggttttag aagccatact
    53701 aaacaatggg atttatcccc aaaattaatt tatattagat aaagctatta atattttatt
    53761 aagatgatat aataaattta atagattaaa gagagcatat gacgggtatt taaaatttaa
    53821 atggtaaaaa tgctcttttt ttattggaca ttcaattaac atgtgtatat aatataaaaa
    53881 taagtattta tttaggttga aatctaataa atatataata ccagtacata agcaaagagg
    53941 agaaaagtaa attatgagac catcatggga tgaatacttc atggaaattg cagaagtagt
    54001 gaaaaagcgt tcaacttgta ttagaagaca agtaggagct gtaattgtaa gagacaaaca
    54061 aatattaaca actggatata atggttctcc gagaaatctt gaacactgtg aaaatattgg
    54121 atgtaagagg caggaattaa atattccctc aggagaaagg catgaattat gcagagcatt
    54181 acatgctgaa caaaatgcga taatacaagc tgctcataat ggtattagtg tagatggggc
    54241 aactttgtat gttactacaa gaccttgtgt tttgtgtgca aaaatgtgca taaatgcagg
    54301 tatattgaaa attgtttatg aaggtgatta tccagatgat atgtctacag aattattaaa
    54361 agaagcagga atagaactca taaaatttta gtggataaaa tttatatgac aataaatgtc
    54421 aaattatgac actgaaatac aatggtatca aatgattaaa agaaagtatt tgaaaaatca
    54481 tcagattagt agtaagatat attttataat taaataaatt tatgagatag attaattaca
    54541 tgttatttat tttataataa tgagggatac tatttatgag taaaagaaat acacatacag
    54601 aaattgcaaa gttgtttagt ttaataagtc aaataggatt gatgattgta atatcaatat
    54661 taggatgtac tttcttagga aaatttttag atagtaaatt gaatactact ccagttctga
    54721 caataatatt tttattgttg ggtgttggag gtgcttttag tggtgtttat aaaactctaa
    54781 tagtatatac acgaaggaag tgatatattg gatataaaat tacttaacca ggtaaggtca
    54841 gtaacaaaag gtatagtaat atttgatgta gttttaattg tgctaatgtt gataacatca
    54901 acattaagta aaccagtatt attaggactt atatttggct ctataatagc tatgttaaat
    54961 tttagattat tggcgatatc actggaaaaa tcagtgacat ttcctgctgg gaaggcacaa
    55021 gcttatgcat cagcacaata tgctattaga ttatttataa tggcagttgt tctaattgca
    55081 tcagctacag taccacattt aaatataatc ggaacagcat tgggattatt gagcacaaag
    55141 tttgtcatat tatcaaaaaa ctttataaat aaattaaaga ggaaggaggc ataagtatga
    55201 atcaagcgca ttattatctc ataggaaact ttaagattag tgaaacaata ctagtaagtt
    55261 ggggaataat ggccttttta gcgatagcat cttatttttt gactagaaac ttaaaaaaag
    55321 ttccaactag taaagttcaa atattcttag aatttgcagt aggtggtctt gctaaaatgg
    55381 ttacagatgt tatgggtaaa aatacagtaa aaagaatgcc atatatgata ccatacatag
    55441 gaagcttgtt tttatttttc gcatgttcaa atttaagccc attacttgga ttaaggtcac
    55501 caacaaccga cttagacaca actgctgcat gggcaatgat tacatttttt atgatttatt
    55561 ttgcaggagt taaatttaag gggttatcat actttaaaga attggctcaa ccacttgcca
    55621 taatgttacc aatcaatatt ataggtgagt tagctagacc aatatcatta agtttccgtc
    55681 catttgggaa tattttaggt ggaacaataa taatgggtct gttttatcaa cttttaggtt
    55741 ttatatctag tttaatacca ggattatcaa taccaattgg agaaatatta ataccagtcc
    55801 cattacactt gtactttgac ttatttgcag gattgttaca agcatatata tttataatgt
    55861 taacaatggt atttatatca agtgcaggag agttagatta agaaatcaac ttatattaag
    55921 ataaaaataa aataatttaa aaaattaaag ttttaaggag gaaataatta tggaaacagc
    55981 aatagtagcg gcagcatcag caataggagc agggatagca gttgcaacag gtataggtgc
    56041 aggtataggt caaggtatag cagcagctaa agctgcagaa gcagtaggaa accaaccaga
    56101 agctaaaggt gatataacat caactttatt actaggagtt gctatagcag agtcttcagc
    56161 aatatatggt ttggttatat caataatact tctattcgta aatccattct ttaaatactt
    56221 aggaatgtag atttagtcct taaatatttt gagataggtt tataggtaaa tcatctcaaa
    56281 cttataatca tgagatgaac tttatcatct cgtgattata agtagttatg atttatggaa
    56341 aggaggataa ttatggaaaa agcactagta ggtataacgt gggaatttgt gttccaaatt
    56401 gttaatactt ttataatatt tttacttctg agaaagcttt tattcaagcc agtattaaat
    56461 ataattgaat caagagaaaa tgatattaaa agtgatttgg cagaaggcga aaaagcaaaa
    56521 aatgaaggtt tagctctgaa aaaagaatat gagtcaaaaa tcaattttgc caaggatgaa
    56581 ggtcaagaaa tcataaaaca agcaactata agagcagagc agaaatcaga tgatatagta
    56641 aatactgcaa agaaagatgc tcttgatata aaagaaaaag caaacaaaga tatagaacaa
    56701 gaaagacaaa aggttataaa tgaaatcaaa aatgatatat ctaacatagc actacttgca
    56761 gcatctaaag ttattgaaaa agatttagat aaatctaaac atgaagaatt gatagaaaac
    56821 tttataaaag aggtagggga agctaaatga taaatgtaat agcaaataga tatgccgaag
    56881 ccctatttca attaggagaa gaagaaaatt caactgatgt cttattcaaa gaactagaaa
    56941 aagtagtaga tatgatgact aaagttagta aagatttcta caaagtttta aaatcaccat
    57001 tagtttcaaa atcagaaaag aaaaacttgg tagaaattat ttttagtaag gaagtaagtt
    57061 ctaacataaa aaacttttta aaagttttag tagataaaga tagaatatca tatttagaag
    57121 atatcgaatt agcttataaa gaattattaa ataagaaaaa caatgtaatt gatggagtag
    57181 caataagtgc tattccaatg agtgaaacag atattaaaga acttgaagtc aaactttcaa
    57241 ataaatacaa caaaaatgtt actatagaaa atgttgttga caagactata ctagggggag
    57301 ttctagttag aattggaaat gaacaaatag atggcactgt gaaaactcgt cttgataaga
    57361 tgaaagaaaa gctatctgaa gtaatatctt aagaatggcg gtgaacccat gaacttaaaa
    57421 cctgaagaaa taagttctat aattaaacag caaataaaaa attatgagaa taaagttgag
    57481 ttgacagata caggtagtgt tttaacagtt ggggatggta tagcgagtgt atatggatta
    57541 gaaaaagcta tgtctggtga gttgctagag ttcccaggtg aaatatatgg tatggcactt
    57601 aacttggaag aagaagtagt tggagctgtt atccttggag atgacagtga aataaaagaa
    57661 ggcgatatag ttaagagaac tggaagaata gttgaagttc cagttggaga agctttaata
    57721 ggtagagttg taaactcact aggtcagcca atagatggaa aaggacctat agcgtacaca
    57781 aaaactagac cagttgaatc tgaggcacct ggtataatag atagaaggtc tgtttatgaa
    57841 ccattacaaa caggaatcaa atcaatagat tcaatgatac caattggtag aggtcaaaga
    57901 gagttaataa taggtgacag acaaactggt aaaacatcta tagttattga cactatacta
    57961 aatcaaaaag gaaaagatgt tatctgtata tatgtagcaa ttggacaaaa acgttctact
    58021 atagctcaat tagttagctc tttagaaaaa ggtggagctt tggattatac aatagtagtt
    58081 tcagcaacag catctgaatc agcaccactt caatatatag caccatatgc tggagcagct
    58141 atgggtgaag aatttatgta caatgggaaa catgtactta tagtgtatga tgatttaagt
    58201 aaacaagctg ttgcatatag agaaatgtca ttgttactta gaagaccacc aggacgtgag
    58261 gcatatcctg gagatgtatt ctatttacat tcaagattac ttgaaagagc agctaagtta
    58321 tctgatgaat tgggtggagg ttcaatgact gccttgccta taatagaaac tcaagctggt
    58381 gacgtttctg catatatacc aacaaatgtt atatctataa cagatggtca aatatactta
    58441 caaccagagt tattctattc aggtgtaaga ccagcagttg accctggtat atcagtatca
    58501 agggttggtg gttctgcgca aattaaagca atgaaaaaag ttgcaggaac attaaaactt
    58561 gcatattcac aatatagaga acttgcagca ttctcacagt ttggttctga cttagatgaa
    58621 gatactaaaa agagacttgc tcaaggtgaa agaatcgttg agatattaaa acaaggtgag
    58681 catcaaccaa taaaagttga aaatcaagtt atgataatat atgctgttat aaataatcat
    58741 ttagaggata ttccgataga taatatagct agatttgaat cagaattgta tgcatttgta
    58801 gataataatt atccggaaat atcaagaaaa atattaggtg gagaagactt cactcatgat
    58861 ttaacagatg caataaatga atttaaagaa aagtttgttg ttgaagtata atccttttta
    58921 aaaaaggagg taaattaatt ggcaggagct ggaatcaaag caatcaaaac acgtatggga
    58981 actgttgaaa gtacaaagca aataactaaa gcaatggaat tagttgcatc ttctaagctt
    59041 agaaaagcaa aagaaaaggc tgaagattca agagaatatt ttaatacttt atacaataca
    59101 gtaaaagata ttgcccaaac aacacgtgga attagaaatg aatttttaaa agaaagagaa
    59161 gtaaaaaata aatgttatgt agtagtagct ggagacagag gacttgcagg tggatacaat
    59221 tccaatgtat taaaggaatc tgtctctcat atgaaaggta aaaaggaaac agtgattact
    59281 gttggaaaga aagcacatga atttttttct aaaagaaact acaatatagc aaaagatata
    59341 aatagtgttg aagcttgtgg atatgaagat gcgacagaaa tttcacaaac tattattgac
    59401 ctgtataaaa aaggtgaagt agatgaagtt tatattgctt atacaaaatt taaatctgct
    59461 ttatcacaag aagttgaaat tttgaaattg cttccattag tctttgaaaa agatacagac
    59521 gaacaaggaa tggcagtaaa agaagaaaaa caaaatagat caaaagtaca atatatacca
    59581 tcagcagaag cagttttaag ttatattgta cctcagttta tatcaggtac agtatatggt
    59641 ggaattttgg aatcttttgc atcagagcaa ggtgctagaa gaactgcaat ggaatcagcc
    59701 acagacaatg cgaatgaaat gctatctaat ttagagttaa gttataacag agcaagacag
    59761 tcagctgtaa ctcaagaaat aacagagata gttggtggag ttgaagctct aaaataggga
    59821 ggttaggaaa tggcaaatgt aggtaaagta gtacagattg taggagctgt actagatgtt
    59881 aagtttgata gtgaacaaag cctacctaac ttattaaatg cgttagtaat aaagttagga
    59941 gataaagaaa tagttgcaga agttgctcaa catataggtg atgatactgt aagatgtata
    60001 gctatgagtg caacggacgg acttgtaaga ggtatggaag ttgtagatac aggagggcca
    60061 ataagtgtcc ctgttggaga tgagacttta ggtagaatat tcaatgttct tggaaaacca
    60121 gttgatggga aaccagctcc aaaatctgca cctaaattac ctatacatag accagcaccg
    60181 gcgtatgatg aactagaaac tacagcagag atactagaaa ctggtattaa ggtagttgac
    60241 ttactagctc catatctaaa aggtggaaaa ataggtctat ttggaggagc aggagtagga
    60301 aagacagttc taatacaaga gcttataaac aatattgcta agcaacatgg tggtatttct
    60361 gtattttcag gagtaggaga aagaacaaga gaaggtaacg acctttatgg cgaaatgagt
    60421 gagtctggag ttataaataa aacagctcta gtatttggtc aaatgaatga accacctgga
    60481 gcgagaatga gagttgcttt aactggactt acaatggcag aacattttag agatgagcaa
    60541 ggacaagacg ttttactttt cgttgataat atattccgtt tcacacaagc tggttcagaa
    60601 gtttcagcac ttctaggacg tatgccatca gctgttggtt atcagccaac attagctact
    60661 gaaatgggtg cacttcaaga gagaataaca tcaactaaga aaggttcaat aacatctgtt
    60721 caagcagtat atgtaccagc agatgactta actgacccag caccagctac aacattctct
    60781 cacttagatg caaaaacagt tttatcaaga caaatatcat cactgggtat atatccagca
    60841 gttgaccctc ttgaatctac atcaaggatt cttgacccta gtatagtagg aaaagaacat
    60901 tatgaagtag ctagaggtgt acaatcaata cttcaaagat ataaagaact tcaagatata
    60961 attgctatac ttggtatgga tgaattatct gatgaagata agttaatagt tgcacgtgct
    61021 agaaagatac aaagattctt atctcaatca ttcacagttg ctgagcaatt tacaggaaat
    61081 ccaggtcaat atgttcctgt taaagagact gtaagaggat ttaaagaaat tcttgaaggg
    61141 aaacatgatg acttaccaga gtcagcattc ttatttgtag gaacaataga agacgctgtt
    61201 agaaaagcga agggaagtat gtagtcatgg ccagtgaatt ttcattgaaa gttgtaactc
    61261 caagtaatac tttttatgat ggagatgttg aaatgatagt agttagaact acaaaaggag
    61321 acaaaggggt gttgaaaggt catataccat ttgtttcagg tatacctgta ggaactttaa
    61381 agataaaaaa agagggaagt tttaaagagg cgaaaatagc agaaggattc attaatgtaa
    61441 ctcaagataa aactgtcata ttgacagaat ctgctgagtg gatttaataa attaatttag
    61501 agtataaaaa gtcattttga ccaaaaaaac ttttgggtaa aatggctttt ttgtgttttg
    61561 gtgttaataa aataacgata aaattgtaag tttttagtga taataaattc acaaaatgtt
    61621 taaatttact gccaaattat gttgtaatta ataagataaa gaagatttag atagctttta
    61681 tgtaagtgaa aaagtcttca tataagaaat acatataaaa tttttataaa atttaaaaaa
    61741 tttgcttagt tgtgtagtat aataaataat ataatagatt tgtatataat attcattaca
    61801 aataatttta aaagttacag ttttattata atgattatta aattaagata agtaacataa
    61861 actaaataat aatgtgtatt atcgatagta aatttataaa ttcattatta attattgaaa
    61921 gaagtattat aaattttctg gaggaacaat gaatatattt gatattggcg tagatataat
    61981 tgaaattgat agaataagaa aggctgtaga caaaaataat aggtttttgg agaagatatt
    62041 tacagataga gaaatagagt attttaattc taagaatttt aaagcagaaa gtatagcagg
    62101 taatttcgca gccaaggaag ctataagtaa atctataggt acaggaatta gattgtttaa
    62161 ttttaaagat atagaagttc tcagaaatga aatgggaaaa ccaatagtaa aaacttataa
    62221 taatctagca aaaatgtgca tagattataa tgttttagaa ataaaagttt caatatcaca
    62281 ttcaaaagat tatgctatag caaatgcgat aaccataatt aaggactaag gagtgattac
    62341 attatgatgg ataaaacagc aaaagaaata atgacaacag atgtaatagt agcaaaacaa
    62401 gatgatagta tagcagatgt agctaatatg ctaattgcag aaaaaatagg aggtctgcca
    62461 gttgtagatt cagagaatag agttgttgga ataatttctg aaacagatat tcttaaaaaa
    62521 gaaaaatata tagaagcacc tctctatata aatctattac aaggattgat tttcttagat
    62581 gacttaaaaa aagttgaaaa agatataaaa caagtagctg cttataaggt tggagaactt
    62641 atgtctaaag atataataaa ggttcatgaa gatgataagt ttgatgatgt agctaatgta
    62701 atgattaaaa aatcaataaa cagagttcct gtagtagatg atgacaacaa acttaaaggt
    62761 ataatttgta gatatgatat aataaaagct ttatacaatg aataaactga agtaggaggt
    62821 gtgatgtgag aaaaaagtgg accatagtat gtattatgtt cttggcactg gttataattg
    62881 taataggatg ccaaaaacga cagtccacaa aagaagaagt atacaaagat tttcaaaagc
    62941 agatttctga tatgaattat tacagttgta aagctgaagt agaagtagtt ggaaataaaa
    63001 gcccacacaa ttatgtttta attcatactt ataaaaaaac cgataactat aaattagaag
    63061 taatttcccc aaaacattta aaagggaaaa gtatagaata tcaaggtgat aaaatattag
    63121 ttaaaaatcc aaagattagt gatgtagtgg agttacctaa tacaggaaaa aataatcaat
    63181 atctttttgt aggagacttt ataaaaaact atcttcaaaa tgaggagatg aaagtaaagt
    63241 tatccaaagg acatttggtt ttagagactt ttattcctgg ggataataaa tattttaaca
    63301 agcaagtatt atatgtaaat gctgacacaa aaaatcctga aaaaatggaa gtgttagata
    63361 aagagggagt gccaagattt acagtaaaat acaaagattt tgaatacaga aactaagggg
    63421 gactaaagac atgcaaaaaa taacagtgcc tacatgggca gagataaatc tagataactt
    63481 aagatttaac ttaaataata ttaaaaattt attagaagaa gatattaaga tttgtggagt
    63541 aataaaagct gatgcatatg gacatggtgc agtagaagtt gcaaaattgc tagaaaaaga
    63601 aaaagtagat tacttagcag tagcaagaac tgctgaagga attgaactta gacaaaatgg
    63661 cataacactt cctattttga acttgggata tactccagac gaagcttttg aagattctat
    63721 aaaaaataaa ataactatga cagtttattc tttagaaaca gcacaaaaga taaatgaaat
    63781 tgcaaaatct ttaggagaaa aagcctgtgt tcatgttaaa atagactcag ggatgactag
    63841 aataggtttc caacctaatg aggagtcagt acaggaaata atagaattaa ataaattaga
    63901 atatatcgat ttagaaggta tgtttactca ttttgctaca gctgatgaag taagtaaaga
    63961 gtacacttat aaacaagcta ataattataa atttatgtct gataaattag atgaggctgg
    64021 tgtaaaaata gctataaaac atgtatcaaa cagtgcagct attatggatt gccctgattt
    64081 aagattaaat atggtaagag caggaataat attatatggt cattatccat ctgatgatgt
    64141 atttaaagat agattagaat taagaccagc catgaaatta aaatcaaaaa tcggacatat
    64201 aaaacaagtt gaaccaggtg taggaataag ttatggacta aaatacacaa ctacaggtaa
    64261 agaaacaata gctacagttc caataggata cgcagatgga tttactagaa tccaaaaaaa
    64321 tccaaaggtt cttattaagg gagaagtgtt tgatgtagtt ggtagaatat gtatggatca
    64381 aataatggtt agaattgaca aagatataga cataaaagtt ggagatgagg ttatactatt
    64441 tggagaaggc gaagttacag ctgagcgtat agctaaagac ttaggaacta taaactatga
    64501 agtgttatgt atgatatcaa gaagagttga ccgtgtttat atggaaaata atgagcttgt
    64561 acaaataaac agttatttgc taaaataaaa ccatgatatt ataatctggg aggcgatttt
    64621 gtgtacgata agagtaaaga aaaaatagaa gttactttac ctgactctct gatttctgaa
    64681 gttgatagta tagttcagat ggagaacagt aacagagaag attttgcaaa agctgctttt
    64741 cagttctata taactcagaa aaagaaaatt catattaaag aatctatgaa aacgggttat
    64801 agagaaatgg gtcaaattaa tctgtcacta gctgaattag gtatgacagt tgaagaagta
    64861 tcttctgatg ataaaaatga agggaagatt gcccaaggtg agtactaatt gtgaaataaa
    64921 gcgtggtgat ttatattacg ctgatttaag tccagttgtt ggttcagagc agggaggcgt
    64981 tagacctgtt ctgataatac aaaatgatgt aggtaataaa tatagtccta cagttatagt
    65041 atcagctata acatctcaga ttaataaagc caaattacct actcatgtag agataagttc
    65101 caatgaatat gggcttaata aagactcagt aattctttta gagcaaatta gaactatcga
    65161 taaaaagaga ctgagggaaa aaattggttg tctagatgaa aatatgatga taaaagtaga
    65221 tgatggactt caaataagtt taggattgtt tactttttaa agagggattt gtcttttatt
    65281 agacaatccc ttttttcttt taaatatctc ttaaggttta attttaatgt actatgttaa
    65341 taaaattaaa aataacgcaa aaaaatagca taaaagtgat ataataatat ggaattgata
    65401 taaaattacg aactcaaggg aggtaattgc atgagagacc ataaaggatt aaatgagatt
    65461 gcgcgtatca taagaagaga tatagtttca atgatacata gagccaaatc tggacatcca
    65521 ggagggtcat tatcagtagt tgaaatactt actgcgttat actttgatga aatgaatgtg
    65581 gattcttcaa atcctaaaat ggaggataga gatagatttg tattatcaaa aggacatgct
    65641 gcaccagcat tatatgctac attggctgaa aaaggctatt ttgataagga agaactgaat
    65701 ggattaagga agataggaag gatgttacaa ggtcatccag acatgaaagg cacgccaggt
    65761 gtggaaatat ctactggttc tttaggtcaa ggtttttcag tagcatgtgg tatggctatg
    65821 gcatcaaagt tggataatgc tccgtggaat gtgtatactt tgcttggtga tggtgaggtt
    65881 caagaaggaa ttgtttggga agctgctatg agtgctgcac attataaact tgataattta
    65941 attgcttttt tagataataa tggacttcaa atagatggag atatagaaag cgtaatgagt
    66001 ttaggttcaa tagttgataa atttaaagct tttggttgga atgtaattga gatagatgga
    66061 catgattttg accaaatatt tgcggctctt gatatagcta aatcaacagt taaaaaacct
    66121 acaatgatag ttgcaaaaac tataaaaggc aaaggaatat cttttatgga aaatcaagca
    66181 ggatggcatg ggacagctcc aagtgatgag gagctagaaa aagccttgct tgaattagga
    66241 ggtgctgaca atgagtaaaa tggcgacgag agaagcatat ggtaaagcat tagtaaaatt
    66301 aggtcaaata aatgacaatg ttgtagtatt agatgcagat ttatcaaagt ctacaaaaac
    66361 acatgatttt tacaagtcat ttccagatag attttttaat atgggtatag ctgaacaaaa
    66421 tttaataggc gctgcttgtg gattatcaac tgctggtaaa ataccttttg caagtacttt
    66481 tgctatgttt gctacgggaa gggcttttga gattataaga aattcagttt gttatccaaa
    66541 gctaaatgta aaaatatgtg ctactcatgc aggtcttact gttggagagg atggagcatc
    66601 tcatgaaagt gtggaagata ttgctataat gagagctata ccaaatatga cagttcttgt
    66661 tccagctgat ggagttgaaa cagaaaaaat tatttttgaa atagctaagt ataatggacc
    66721 tgtttatgtt agattaggga gaagttcagt accagtttta tttgatgaag attataaatt
    66781 tgaaatagga aaaggtacag tcttaagaga aggaaaggat gtatcaatta tagcttgtgg
    66841 aattatggta aatgaagctc tattagcaca agaaaagttg caagaagaag gtataaatgc
    66901 tagagttata aacatgtcct cgataaaacc tatagacaaa gatttaatat tagaatcagc
    66961 aaaagaaacg aatgctattg taactgtaga agaacatagc ataattgggg gattaggttc
    67021 tgctgtaagt gaggtagtag gagagtcttg tccaaccatt gtaaagaaag ttggaattaa
    67081 agatacattt ggagaatctg gtactccaaa tgaattgtta aaaaaatatg aattaacttg
    67141 tgatgacata ataaagactg ttaaagaagc tattattgca aaaagaatgt aagagatata
    67201 aattattcat gtttgttaat aatataatat atttaaaaga gaggtataaa gtgttatatt
    67261 aattta
//
LOCUS       DAGQWR010000002        61631 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.002, whole genome shotgun sequence.
ACCESSION   DAGQWR010000002 DAGQWR010000000
VERSION     DAGQWR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.002"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(135..1736)
                     /locus_tag="MIW95_000070"
     CDS             complement(135..1736)
                     /locus_tag="MIW95_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBY3149430.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDDLRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDNKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGIIKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(1847..2524)
                     /locus_tag="MIW95_000071"
     CDS             complement(1847..2524)
                     /locus_tag="MIW95_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149431.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(2537..3232)
                     /locus_tag="MIW95_000072"
     CDS             complement(2537..3232)
                     /locus_tag="MIW95_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBY3149432.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(3284..4483)
                     /locus_tag="MIW95_000073"
     CDS             complement(3284..4483)
                     /locus_tag="MIW95_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBY3149433.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDICRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(4744..6789)
                     /locus_tag="MIW95_000074"
     CDS             complement(4744..6789)
                     /locus_tag="MIW95_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3149434.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNILIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILNSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(7200..8009)
                     /locus_tag="MIW95_000075"
     CDS             complement(7200..8009)
                     /locus_tag="MIW95_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY3149435.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTFVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(8093..8626)
                     /locus_tag="MIW95_000076"
     CDS             complement(8093..8626)
                     /locus_tag="MIW95_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBY3149436.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGLNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(8753..10828)
                     /locus_tag="MIW95_000077"
     CDS             complement(8753..10828)
                     /locus_tag="MIW95_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3149437.1"
                     /translation="MLKGLSFRNARRQAGKYLIYWCSLIGSVALIYAFNALIFSDVMQ
                     ELSSILNNGGSDEFSYMIIIFSFIITFILGWFVSYMMNFILKKRSREISTYMILGVDK
                     KQIVKMLFIENLLLGASALIVGWILGIFVSKVLESIVINMFHGKYSLSPDFSLKAAGM
                     TFLYFCIIYLIAQLKSNKKLVKMKLINLLNYDRYNENVLIHQTKTKSIMFCVSVLCGG
                     MGIFFFSIPSGRLTDVFLGLLLIVFCLIVLFMGFGSIAQNMFEKGMNWKYKKSHLFLY
                     RLLASKINGISIIMGIIATLFTVSIACIGIANSFYSVMNKSVDLQQFDVSIFHPNESY
                     DFSSYEGYLSETKGVKKDYSYTLYTEEKTSFMKVRNNILSKYLKDIGRDLQYKDYTFA
                     ENQFDTFMKYSDYNILRNMLGLNPIKMDKNQFIIHCMPYLADAYKNYIGEKYKLTISD
                     KDLSIAGIYIENFSQYGGYGNGQEYIIVIPDYLIDNMKPLYSLYVAITSEPLDSAYFT
                     EFQKKFDTLENLNSGVVVAGNQGFVSKLRYDNKDYISGKYAMQSTGQAIILILPLFYL
                     ALIICIIGTVILSIQLLSENNIIIKHYGMLRTLGMIDSEVIKILKKHVCLYFSIPLIP
                     AVIFGGILVSVFSYNMFNISFDAPVFFNIQIPILHAVAITLINFVVIYCIYVFITYVA
                     LRKEILHIK"
     gene            complement(10831..11577)
                     /locus_tag="MIW95_000078"
     CDS             complement(10831..11577)
                     /locus_tag="MIW95_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3149438.1"
                     /translation="MLLRVENIEKYYGNQKILTKALNDVSFQINTGEFVSIMGTSGSG
                     KTTLLNCIATIDTVSAGKIYLENQDITELSEENLTVFRRDNLGFVFQDSNLLDTLTLE
                     ENIALPLTIKKVSPVEITNSVYNIATQLKIEEILSKFPYEVSGGQRQRCAVARAVVTE
                     PKLILADEPTGALDSNSSKTLMELLETINTILEATILLVTHDAISASYTERILFLRDG
                     KIFNELRKGEKSRLTFYHEILDALSLMGGD"
     gene            complement(11591..11986)
                     /locus_tag="MIW95_000079"
     CDS             complement(11591..11986)
                     /locus_tag="MIW95_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBY3149439.1"
                     /translation="MKKRILTILCVVLVLFISITGLDSMGFFSRSIIPLRSEYASFIR
                     KNGTITEVKFTGPFNHNNIDLNKNVEDTNKNGDDNELTIKYDIEVKKSLISSNEPTIQ
                     IQDSKESIYVYILNFSDKTIIIKNGKIVD"
     gene            complement(12099..13121)
                     /locus_tag="MIW95_000080"
     CDS             complement(12099..13121)
                     /locus_tag="MIW95_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY3149440.1"
                     /translation="MRFCNFLQDKVLVILLNICCAISLVLYLHLIGIGQGELTLLIAA
                     WVAILFCWICFCYQKQKKEIIKLRRIVDELDKKYLFIEMIEKPTSNIEREYYNIIRLS
                     LKSMTDHVSQIHREMYEYRQFIEQWVHEIKHPVTTSELICENYKNEHTRKIRFQMEEI
                     ERHIERALYYARLGHVEKDYLISEITLKSIVEEVLAKNKLLLIQNNIHINIQDLEYSV
                     YTDKKWIVFILNQIIINSVQYKSDSAEISFFAIKEDKSVKFTIKDNGIGIKESEIYRI
                     FELGFTGSNGRTTNNSTGIGLYLCYELSKKMGIDIDVSSSLGEYTAITLNFPINTAIN
                     MQKQCN"
     gene            complement(13118..13789)
                     /locus_tag="MIW95_000081"
     CDS             complement(13118..13789)
                     /locus_tag="MIW95_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3149441.1"
                     /translation="MILTNILIIEDDVFICKELMQFFENNGYSTQTLDTNNCDVLQVI
                     QESTPDLILLDINLPNIDGFSLCRQIRNCSTIPIIFITARDSAIDELNGLTMGGDDYI
                     TKPYNLPLLLARVKNLLKRVDSSDKQKIKYREIILNLISATVEYQNAVIELSRTELKI
                     MYYLFQNVGSIVPRIDLVEYLWDNHIHIDDNALSVNITRLREKLRGIGITDLIITKRG
                     MGYRI"
     gene            complement(13875..15224)
                     /locus_tag="MIW95_000082"
     CDS             complement(13875..15224)
                     /locus_tag="MIW95_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBY3149442.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNRANSIEEIKPCEISKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIDKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEVIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(15236..16618)
                     /locus_tag="MIW95_000083"
     CDS             complement(15236..16618)
                     /locus_tag="MIW95_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBY3149443.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            16862..18076
                     /locus_tag="MIW95_000084"
     CDS             16862..18076
                     /locus_tag="MIW95_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBY3149444.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIKLYGNKCDYAKRDTLLYTANKLEIKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            18253..20166
                     /locus_tag="MIW95_000085"
     CDS             18253..20166
                     /locus_tag="MIW95_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3149445.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDNIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            20437..21411
                     /locus_tag="MIW95_000086"
     CDS             20437..21411
                     /locus_tag="MIW95_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBY3149446.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            21432..21707
                     /locus_tag="MIW95_000087"
     CDS             21432..21707
                     /locus_tag="MIW95_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBY3149447.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            21720..22883
                     /locus_tag="MIW95_000088"
     CDS             21720..22883
                     /locus_tag="MIW95_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBY3149448.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGTEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            23220..25499
                     /locus_tag="MIW95_000089"
     CDS             23220..25499
                     /locus_tag="MIW95_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY3149449.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVNFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSVVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPMPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(25594..26934)
                     /locus_tag="MIW95_000090"
     CDS             complement(25594..26934)
                     /locus_tag="MIW95_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY3149450.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILIGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVTYYFIR
                     GKSTLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     IVQVFPEVFVGIFNKDSDLMDIAVRGISINLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(26946..27728)
                     /locus_tag="MIW95_000091"
     CDS             complement(26946..27728)
                     /locus_tag="MIW95_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3149451.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(27743..28765)
                     /locus_tag="MIW95_000092"
     CDS             complement(27743..28765)
                     /locus_tag="MIW95_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBY3149452.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(28771..29784)
                     /locus_tag="MIW95_000093"
     CDS             complement(28771..29784)
                     /locus_tag="MIW95_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBY3149453.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(29912..30850)
                     /locus_tag="MIW95_000094"
     CDS             complement(29912..30850)
                     /locus_tag="MIW95_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY3149454.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWSQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            31120..31584
                     /locus_tag="MIW95_000095"
     CDS             31120..31584
                     /locus_tag="MIW95_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149455.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTGKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(31696..32013)
                     /locus_tag="MIW95_000096"
     CDS             complement(31696..32013)
                     /locus_tag="MIW95_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY3149456.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(32026..32757)
                     /gene="chbG"
                     /locus_tag="MIW95_000097"
     CDS             complement(32026..32757)
                     /gene="chbG"
                     /locus_tag="MIW95_000097"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBY3149457.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVVEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(32783..34093)
                     /locus_tag="MIW95_000098"
     CDS             complement(32783..34093)
                     /locus_tag="MIW95_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBY3149458.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNNGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLVAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(34170..35483)
                     /locus_tag="MIW95_000099"
     CDS             complement(34170..35483)
                     /locus_tag="MIW95_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY3149459.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(35505..35816)
                     /locus_tag="MIW95_000100"
     CDS             complement(35505..35816)
                     /locus_tag="MIW95_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3149460.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(36016..36720)
                     /locus_tag="MIW95_000101"
     CDS             complement(36016..36720)
                     /locus_tag="MIW95_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY3149461.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(36815..38176)
                     /locus_tag="MIW95_000102"
     CDS             complement(36815..38176)
                     /locus_tag="MIW95_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY3149462.1"
                     /translation="MKDRINLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAIYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     MSE"
     gene            38675..39820
                     /locus_tag="MIW95_000103"
     CDS             38675..39820
                     /locus_tag="MIW95_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY3149463.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQDVINKKYIVYRIGGDEYIIILKNLSHKEINSIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKKKINK"
     gene            complement(39910..41628)
                     /locus_tag="MIW95_000104"
     CDS             complement(39910..41628)
                     /locus_tag="MIW95_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBY3149464.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(41633..42622)
                     /locus_tag="MIW95_000105"
     CDS             complement(41633..42622)
                     /locus_tag="MIW95_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY3149465.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(42718..43767)
                     /locus_tag="MIW95_000106"
     CDS             complement(42718..43767)
                     /locus_tag="MIW95_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY3149466.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTYNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(44044..44712)
                     /locus_tag="MIW95_000107"
     CDS             complement(44044..44712)
                     /locus_tag="MIW95_000107"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBY3149467.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(44718..46091)
                     /locus_tag="MIW95_000108"
     CDS             complement(44718..46091)
                     /locus_tag="MIW95_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBY3149468.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(46088..47866)
                     /locus_tag="MIW95_000109"
     CDS             complement(46088..47866)
                     /locus_tag="MIW95_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBY3149469.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(47983..48306)
                     /locus_tag="MIW95_000110"
     CDS             complement(47983..48306)
                     /locus_tag="MIW95_000110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBY3149470.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(48293..49270)
                     /locus_tag="MIW95_000111"
     CDS             complement(48293..49270)
                     /locus_tag="MIW95_000111"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBY3149471.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(49285..49848)
                     /locus_tag="MIW95_000112"
     CDS             complement(49285..49848)
                     /locus_tag="MIW95_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBY3149472.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(49900..50391)
                     /locus_tag="MIW95_000113"
     CDS             complement(49900..50391)
                     /locus_tag="MIW95_000113"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBY3149473.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(50394..52319)
                     /locus_tag="MIW95_000114"
     CDS             complement(50394..52319)
                     /locus_tag="MIW95_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBY3149474.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(52321..52632)
                     /locus_tag="MIW95_000115"
     CDS             complement(52321..52632)
                     /locus_tag="MIW95_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149475.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VRKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(53262..53537)
                     /locus_tag="MIW95_000116"
     CDS             complement(53262..53537)
                     /locus_tag="MIW95_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBY3149476.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DITVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(53652..55061)
                     /locus_tag="MIW95_000117"
     CDS             complement(53652..55061)
                     /locus_tag="MIW95_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBY3149477.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     SLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGGIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(55431..56093)
                     /locus_tag="MIW95_000118"
     CDS             complement(55431..56093)
                     /locus_tag="MIW95_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149478.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(56347..58566)
                     /locus_tag="MIW95_000119"
     CDS             complement(56347..58566)
                     /locus_tag="MIW95_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY3149479.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYALNQDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKITISVPLLNNDK
                     VVGVLYCTYNINTLMKLINVSFYENNSISYVVRNDGTIILHPQEDSLSKNIYKLLKQD
                     NDIREINRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSEQIINRAIYAVLFIILIFIAIIFYIIYIKKSNEKEILSLAYEDKVTHIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGKNKRIRKIDSIRNNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LSEWKKQGKKIVPVSINLSRVELYQPDIVKFINKTIQMYNLSSDLIEIEITETVAINE
                     LNILKNVLNELRKYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLNGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(58842..61496)
                     /gene="adhE"
                     /locus_tag="MIW95_000120"
                     /gene_synonym="adhC"
     CDS             complement(58842..61496)
                     /gene="adhE"
                     /locus_tag="MIW95_000120"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBY3149480.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYTQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDESADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDNAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRSPGKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEEPKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT        20577 a        10738 c         7419 g        22897 t
ORIGIN      
        1 ttatctgatt attaaaataa atattaatac tataaataaa aacgaagcat gatttttatt
       61 taaataaatc ccaaacttcg tctttactta cttagatatt tatttacttt ctaaatccta
      121 ttatttttaa aaccttatct ttttaatact tcaccacatt tattctcagt aatctctcca
      181 ttttctaacg caatttttcc atttacaatt acatattcaa caccattggc tagtaattgt
      241 ggctttgaaa aatcaatatc ttctgaccta gaataactat agttatccat gtcaataact
      301 gctatatcag ctatcaaccc ttctttaata atacctctat tttttatttt aaattgccta
      361 gctggtaatg aagtaactct tcttacaatc tcttccaatc taacaccaga ctttctgtac
      421 atattgaaga aaattggaaa agctcctctt ctttgtagtt caaaccatga catatcccca
      481 cccacactaa acaaacaatc tgaaccaacc ataacaaatg gattatcaat tattttatta
      541 tcatgtagtt tatctgggaa atcagctcta tacattcctc ctaaacaaaa gataagttct
      601 tcgtctccat ctaaaagtaa atcgagtaat aacttgtcct catctattcc tttttgtgta
      661 gctatttcac gtattgattt cttttccata ttaatatctt tagtgtttag aagtattata
      721 ctccccctat ctccaagtat aaatgcttct tttaatataa gtgttcttcc ctcttcattt
      781 ctaagatcat ccattacccc atctatacct aattcaaata gactagatga tattgccata
      841 ataaattgaa ttactctttt tttcttcata caatgcccag aacttcttgg tactgtgtca
      901 accattattt caacaccatt atacctagcc tttaatatct catcaaatgc ttctatttca
      961 actggactta agtgagatac ttgaacccta gcaccacttt tttgcccaac ctctatggct
     1021 tcttttactg cattatatct tcctatgtaa tctctaatat gagatgtata tataccatca
     1081 tgctctttaa tctgccttgc aatatcaaca agttcatcaa tatcagcata cttacttggt
     1141 atatatgcca atccagtaga tattccaaaa gcaccacttt tcattccatc atttattata
     1201 tctgtaattt catttttttc cttttctgta ggaggtctat ccttagaacc tcccataaca
     1261 ctccatctta tagtcccatg ccccaacaag aatcccatat tgacagaaat accactttta
     1321 gatataaggt cacaatattc tgtaaaatta ttccaacatt tatttttatc tataagatac
     1381 tttttttctt cttcaagtaa tagaccattt ttatacatat attcatatac attttcagaa
     1441 gaatatggtg ttatagaatg tccacaatta ccatttatag ttgttgtaac tccttgtttt
     1501 agaaactttt caaatttacc atctagtatt gctactattt cttcatgaac atgagggtca
     1561 ataaatccag gtgttataac ttttccacaa caatcaatct ctttatttgt attgctattt
     1621 atcttagaat ctatttttgc tattaagttg tctttaattg ctatatctcc ataaaaacca
     1681 ggattacctg taccatctat tataaatcca ttttttaaga ttaaatcata tatcaaaata
     1741 ttccctcctt aaaaaccaat ttttaaataa tcttaatgta atttttatat ttgaaaatca
     1801 aattattata tttcatattt gaaaattcac tattatttca ttgtaattag tcactaagct
     1861 tatctttata catagctctt gcaattaata tagtaccaaa tacacatata agaactacta
     1921 gagactgtgg tattatacct tgctttgata atataatagt caatacagat attacaattg
     1981 ctactagacc taatttttta tcttgtaaaa atacttgtcc aaaaactgac ccaaatagag
     2041 ctggtaaaat taagtttaat ttttctacta cttcagctgg aattttagat aatacgcttc
     2101 ctcctaaaat tacccctata gttagtatac ttatattcac taaaatagat acagctattc
     2161 ctattgtaga tattatcgag ccttcttctg tacctgattc aacttctgct gccttttgag
     2221 ctgctattga acatggtagc ctcatattag atatattacc agaaagaaaa ctcatataag
     2281 ttcctgggat acctaagatt ggtgaataag aaactggttc tattatgtaa tttggagcac
     2341 tcatactgac aattgccatt gtacttgcta ataatgctga tattggaggc ataataccaa
     2401 atccaaagct caaaattatt ccaggaacta atgctgctat aatacctaaa gatagtgtta
     2461 gtcttccata ccttatcata aaaggtaaat attcttcatt gtatatagat tttactttac
     2521 tcataaaact ccccccttat attactgaag ctattatcat tccgctaaac attgatattg
     2581 taagccccca ctctcttaac cattttatat ttttcttctt ttcaagcata cacaatatta
     2641 ccattatagc cattccactt atacatgcaa ttgttccatt atcaaaacgc aacaccctat
     2701 cagcatttag atatgcaaat gaacccaaca ttgctcctag agatattatt ggtataaatg
     2761 atttcttgcc actagatata acattggtta tcttatctag tttatgtcca aatagtaatg
     2821 tgaatattat ccatcctaaa gaacctaata tcatagtcca aactgcattt gtaaatgcaa
     2881 gttttgtcat ttcaggagaa cctaaagtta tacctaatgc atctgttcca aatccagctg
     2941 acattgattc aaaaattaca gaacctataa atgataatct catccatgct ataggcccac
     3001 ccatagttac tataagcgat accattccag caaatatagt cattgaaggc ccaatagctg
     3061 aaatagcact actcttaaca gctgacttta tttgtttatc tgttattccc attgttttac
     3121 caactttgca agattttata gcaaatataa gggattgtac cactactatt gatactgcta
     3181 ctgtactcgc tatccacaaa agtggatgat tagctaattt taaataatcc atttataaaa
     3241 cctccctata aatcctcaaa ttttattaat atttctaata tatctatttc caaacctgtt
     3301 ttagtactct aagtatattt ccacctagta ccttctctat ttcctcatca ctatatccat
     3361 gctttactaa atatctgaca atgttttttg aagtttctgt tgggttctca agacctttta
     3421 catattctac tttgggatgt tcttttgtac ctgttatttt ttttatatca aatttagcag
     3481 ctagtactga gtgtaatttt acatggtcgc catatactgt atctggtcca aaagctacat
     3541 ggtctattcc tactaaattt ttaatatact caaaatgttc cataaatgac tctattgaat
     3601 gttctttatt cttctctgtt attgtagtat gaggtgctgc ttctacacct ataacccctc
     3661 cttttttagc acatgctatt aatacttcat ctggagttag tctctttgtg ttccataaat
     3721 ttcttgcccc aacatggctt aatattattg ggtccttgct atattctatt acatcaagtg
     3781 tagtttgaac tccaacgtga gagcaatcta ttgccattcc aattttattc attctttcaa
     3841 cagctttttt tccgaaattt gtaagacctc catctttctc ttcttttagt cctgaaccta
     3901 atgcattgga ttcgctgtat gttataccca ttaaccttat accaaatcca tacagtatat
     3961 ctattctatc aagttcattt tctattggtg ctgcaccttc taatgttggt atcaatgcta
     4021 ctttaccttc acttttagct ctgcatatat cttctacttt ttcacacttt atgacaaaat
     4081 cttgatgagc taaatcacat agtctcatgc ctaaatctat taatatatca tcccatttcc
     4141 accctgaatt tgatgttatc gtacatacac catccattaa attatcaaat atagcatcat
     4201 aatatcctct tgataatgct tcataagcgc atctttgttt accttcccta ttatattcaa
     4261 atatatctct ttctaggtgt tctggaaata atactggatg ttcatgtaat gatataaata
     4321 ttttttcatc cactatactt ttaaccttag cttcttgaat atcatctaat tcaataaggt
     4381 actctttaac tcttttatct cctttacaca tctcaataga tgaaatatct tttccttttt
     4441 ctaaataact ataggcttca tatcctttgt aagctgactt cataacaaca tcctccaagt
     4501 tctacaattt tttatatttt tcctatgtag caattgatat gccaaaatat aaattttaat
     4561 tgtataataa atcttgcaaa aaataaaaac agtagtaagg ttgatattta ctactgtttt
     4621 attaaaaata atcttcttag attatttatt caatattttt gaattatctt aatcttaaag
     4681 ataattttat ttatttctat ccattatagg gattttttta gggtgttttg cctttttagt
     4741 cccctatatc actcccttaa tttttacata tgatataaag ttctttcctt catcagttat
     4801 aaaactacct tgttttttta ctccagataa aagtaaacca tggctcttta acttatctat
     4861 tctttttcta atttggtcta cacttaaaac tatatctttt tcttttaaaa tctcttgcag
     4921 tttatttctt cctaataata tatttttatt attccaagtt tctatactgt tcaatataca
     4981 tatactttcc tcaaagaaat tagattgttt gaaatctaag ataattgaat cgaaattttc
     5041 attaactaaa gtattgtttt tttcaaatct aaattgttct ggcaaatgct cgtaatctac
     5101 tctatcttcc tcaactatag tatcaatata ataaattaaa tttttaagct ctcttacatt
     5161 tcctggccat tcatacaatg ttaaagcttg cattgattcc tctgtaaaac atttgttgct
     5221 atttatttca tctaaaaaat acttacttat aagaggtata tcttccttac gttctctaag
     5281 tctaggtatc tctatatgta atacattaat cctataatac aaatcttctc taaaagaacc
     5341 ttctttaatt tttttcttta aatctttatt tgttgcagct ataattctaa catctattgg
     5401 tataatttta cttccgccca ttctcataac ttctttttcc tgtaatactc ttagcaatct
     5461 ctgctgtaca tctaaagata tatcacctat ctcatctaaa aaaattgttc ctgtatgcgc
     5521 tctttcaaat atacccattt ttccaccttt tattgctcct gtaaaacttc cttcatcata
     5581 gccaaaaagt tcactttcaa taagtgtatc tgacaaagaa gatagattaa ctgcaacaaa
     5641 tggcttgttt tttcttagag attcattatg tattgcctgg gcaaatattt cttttcctgt
     5701 tccattttct ccaagtatca acacagaaaa gtctgtagtt gctatttttc ttgcaatatt
     5761 aattttttct tttattattt tactctcacc tacaacactc tcaaatgtat actttgatac
     5821 aaaacctttt gactgaagtt tattttgaat cttgtcctca agcacttgaa ttgcacttat
     5881 atttttaatg cttataatac tctttattcg ttcattattt tcataaacat ttactttatt
     5941 aattatcagt tttttgttat ttaaatttac tacttcatca ttaacttcat cttcttgaaa
     6001 aaatattttt ttgacaacct tatcactaaa taagtccata aagttattag atataatttc
     6061 attttggtct attccaacta gattagaaaa tactttgttg caatatataa attttcctaa
     6121 tttatcaaca gaggcaatac cttcatctat aatctccaaa aaactcttca tggttttatt
     6181 catagttgta taatacctat atccacttac tgtatcttca ttgtattttt ccttaataat
     6241 atgtaattta tctatagata tatttagttt tgtaaagatt tctataactg tatttatatc
     6301 aattacctta tcacctatgt caattatttg ctttatgttt tgtggtatgc tgtttctgct
     6361 tcccgtaata attccaatct cacatttaga cttatcacaa cctggataat atggtattag
     6421 attaatatga attaatccta gttttctaat tatcttggca gtttcatctg ctgactcttt
     6481 ataagcatct ataaccataa catcagaatc attttctata cttattatct gatttatatt
     6541 ttcaatattt atagtacgat gtactattac aataagtata tttttatcaa tattattatg
     6601 tatatattcc tttatatcat tgtctgaaca tactacaagc tcatagcttg acagttcaat
     6661 atttatccta tttacatata ccaagttatc tatattacaa aattctccaa atatattttc
     6721 caattgtttt gatatatcta tatttatttt gtcatcagtt gatacaaata aaatattatg
     6781 ttttttcata gtcccacctc ctgtttaacg agtatagaaa gtaataattt ggtctattgt
     6841 agcacttttt aaaaaagttt taaatcttaa aaatcaaatt ttgtaccatt tactattatt
     6901 ttccataata aaataatgta agctatcttt tttcacatat aatttaattt ctaaaacggt
     6961 tagtttgaca ttattggaat ataaacaaaa atttaatata aattcgttaa tatattaata
     7021 catttttaag aatgacaagg aataaattat aacaaatttt aggaatatat aataataggg
     7081 tctcagaact tagttacaag ttctaaaacc ctatttgttt agtgtaaaaa acatcataaa
     7141 atttagctta aatcttttca cataatatta ctctttttct aagtgataat aaaatatatc
     7201 tattcctcta aaaactgtaa aaattcctga ttaaatagtt taagctcatc atagaatata
     7261 gcatgaccac tatactcaaa agtatataaa attgaatctt ctattttttc attcataaaa
     7321 actgcaaatt catacggaca tatttcatct aatttcccat gaaatatacc agtaggcact
     7381 cttacacatt ttaaatcatt aaataattct tcatctctta aagatacagc agttccaata
     7441 gtgccaatac ctgatgcact aaaaccaata tcattaaacc atcttctaaa gctttctggt
     7501 ggatttgatg caaatacttt ttctccaaaa tcggttacca tttctggtct atcagtacat
     7561 gcttgtgcta tcaatttatt tacatcttca cgtgtcatac catatggaaa ttctggaggt
     7621 ctttgtacaa atgatggtgc agcagctgca gctaatacca attttgacac tccatatcca
     7681 ttaaataaac tcatatacct aagtacgatt gctcctccca ttgaaaaacc aacaaaggta
     7741 aagtctttta atccaatagc atgtaccacc ttataaatat cgtctgctaa ttggctataa
     7801 gtatatcctc ctgatgttgc atcagattta ccaaaacctc ttaaatcaat tgaaactgtt
     7861 ctatatccga gttttggcaa tatatttgtt tgatattcaa atatcttatg tcctaagggc
     7921 caaccatgta taaaaagtac agttttttta gcactcggat tcaaatcata tactgctatg
     7981 tttatatcat tcacatatac ataatacata tcaacagcaa ctcccttcaa gaaaatgtat
     8041 gatggttatt ttatatatat gaataaaatg atgaataact aaatttttaa atttatttac
     8101 aatacttctc tattgccttt tcaaataaac ttacatgaag tttttcatct aggattattc
     8161 tatttaatat agcttttata taagggtctt caataagact tatatcttta taataattat
     8221 taatagccac atgctccaaa tgcaaatcta gttttaatct gttacaaata gaatttccat
     8281 aacatacaaa accaccattc caatatgcac cacaagaact tgtagaacct ctatatacag
     8341 gatttccacc caataatctt atagtctttg ctaatatctc catatgtaac atttctgtta
     8401 tagatatatt tatccacatt tcacttaact ctctatgcgt ttcatctaaa tcaaaattat
     8461 ggtatagata ttgatttaca ctcgtaaatt cacttgatac tccggcatag tcatccatca
     8521 ataattctgc ataatactta tttagaccta aaacttttat ctctggatat ggctcatcac
     8581 ttgaatatcc atttcgcttg tgcatgtcgt aatgtgcatc actcataata tctcctcctt
     8641 taaatttaat acttgatatt aattgtatgc atcacgactt tttcatataa cttagataat
     8701 aaaatctatt atattatttt ctattataat ttatgtgtca attcacccaa atttatttga
     8761 tatgcaatat ttctttcctt agtgctacat atgtaataaa aacatatata caatatatta
     8821 ctacaaaatt aataagcgta atggctacag catgtaagat aggaatctga atattaaaaa
     8881 atactggagc atcaaaagaa atattaaaca tgttgtatga aaaaactgat accaatattc
     8941 caccaaatat tactgcagga attaatggaa tcgaaaaata taaacaaaca tgtttcttta
     9001 agatttttat tacttcacta tcaatcatcc ccaaagttcg aagcattcca tagtgtttta
     9061 tgataatgtt attttcgctc aataattgaa tagagagaat gactgtacca ataatgcata
     9121 taatgagtgc caaataaaaa agtggtaata tcaagataat ggcttgacca gtagattgca
     9181 tagcatactt tccacttata taatccttat tatcatatcg taatttagaa acaaagcctt
     9241 gattacctgc tactactact ccagaattaa gattttctaa agtatcaaac tttttctgaa
     9301 actcagtaaa ataagcacta tccaaaggct cacttgtaat agcaacataa agactataca
     9361 gaggtttcat attatcaatt aaataatctg gaatgacaat aatatattct tgtccatttc
     9421 catatcctcc atactgtgaa aagttctcta tataaatacc tgcaatactt aagtccttat
     9481 ctgatattgt cagtttatat ttttctccaa tataattctt atatgcatca gcaagataag
     9541 gcatacaatg gatgataaat tgatttttgt ccatctttat tgggtttaaa cccaacatat
     9601 ttcttagtat attgtaatca ctatatttca taaatgtgtc aaactggttt tctgcaaaag
     9661 tataatcctt atattgtaaa tctcgcccta tatctttcaa gtatttagaa aggatattat
     9721 tacgaacctt cataaaggat gttttttctt ctgtgtaaag agtataagaa taatcctttt
     9781 tcacaccttt cgtttctgat aaatatcctt catatgaaga aaaatcatat gattcattcg
     9841 ggtgaaaaat actcacatca aattgttgta aatccactga tttattcatc acagaataaa
     9901 atgagttagc tattccaata caagcaattg atactgtaaa aagagttgcg ataataccca
     9961 ttataatgct tattccattt atctttgaag ccagtagacg atacaaaaat aagtgagatt
    10021 tcttatattt ccaattcatc cctttttcaa acatattctg tgcaatagat ccaaatccca
    10081 taaataatac gattagacaa aatacaatca acaataaacc aagaaataca tctgttaatc
    10141 taccactagg tattgaaaag aaaaaaatac ccatacctcc acacaaaaca gacacacaaa
    10201 acataataga cttcgttttt gtttggtgaa ttaatacatt ttcattatat cgatcataat
    10261 taagcaaatt aattaatttc attttaacaa gttttttatt actttttaat tgagcaatta
    10321 aatatattat gcagaagtat aaaaaagtca tcccagcagc ttttaatgaa aaatccggag
    10381 ataaagaata ctttccatga aacatattaa taacaataga ttccaaaacc ttagaaacaa
    10441 atatccctag aatccatcca acaataagag cagaagcacc taataaaaga ttctctataa
    10501 aaagcatttt aacaatttgt tttttatcta ctcctaatat catataagta cttatttctc
    10561 tgcttctttt tttcaatata aaattcatca tataactaac aaaccagcct aaaataaaag
    10621 taataatgaa cgaaaatatg ataatcatat aagaaaactc atcgcttcct ccattattaa
    10681 gtatactaga aagttcttgc attacgtcgg aaaatatcaa agcgttaaaa gcataaatca
    10741 aagctacaga gcctattaag gaacaccaat aaataaggta ctttcctgct tgtcgtcttg
    10801 cattacgaaa agataatcct ttcaacataa tcaatctcct cccatcaaag aaagtgcatc
    10861 caaaatttcg tgatagaaag taagtctgga tttttctcct tttctaagtt cgttaaaaat
    10921 tttcccatcc cttagaaata aaattctctc agtataacta gctgaaatag cgtcgtgtgt
    10981 gacaagaaga atcgtagcct ctaatatggt gtttatagtt tccaaaagtt ccattaaagt
    11041 tttagatgaa ttagaatcca atgcaccagt tggttcatct gccaaaataa gtttgggctc
    11101 tgttacaact gcacgagcta ctgcacaacg ttgcctctga ccaccagata cttcataagg
    11161 aaacttcgaa agtatctctt caattttcaa ttgtgtggca atattataca cactattagt
    11221 aatttcaaca ggagaaactt tttttattgt aagtggaagt gctatatttt cttctaatgt
    11281 cagagtatct aataaattag aatcctgaaa aacaaaacct aaattatcac gacgaaaaac
    11341 agttaagttt tcttcggata gttcagtaat atcttgattt tcaagataaa tttttccagc
    11401 acttactgta tcaattgtag caatacaatt taacaaagta gttttaccag agccagaagt
    11461 tcccataata ctgacaaact ctcctgtatt aatttgaaat gacacatcat tcaaggcctt
    11521 tgtaagtatt ttctgattgc cataatattt ctcaatgttc tctactcgta atagcatgtt
    11581 aaagtcctcc ttagtcaaca atttttccat ttttaattat tattgtctta tctgaaaaat
    11641 tcaagatata tacatatatg ctttctttag agtcttgaat ttgaatagta ggttcattag
    11701 atgaaattag agattttttt acttcaatat cgtattttat tgttaattca ttatcatctc
    11761 catttttatt agtatcttct acatttttat ttaaatcaat gttattatga ttaaagggac
    11821 cagtaaactt tacttctgta atagttccat tttttctaat aaaagaagca tattcagacc
    11881 ttagtggtat gatacttcgt gagaaaaaac ccatcgaatc caatcctgta attgaaataa
    11941 acaaaacgag tacaacacat aatatagtta atatcctttt tttcatatgt ttctccctcc
    12001 tttctattga tattgtagcg taaatagtaa tctgaatata tagaagttac ttaactttat
    12061 cttacagttt tgaaagataa agaaatagca gcgcattttt agttgcactg cttctgcata
    12121 ttgatagctg tatttattgg gaaattcaat gttattgctg tgtattcacc aaggctggag
    12181 gacacatcta tatcaattcc cattttctta gacagttcat aacaaaggta aagaccaatc
    12241 cctgtagaat tgtttgtagt ccttccatta ctgccagtaa aacctaattc aaaaattcga
    12301 taaatttcac tttctttgat gccaatacca ttatctttta tagtaaactt aacactcttg
    12361 tcttctttta ttgcaaaaaa tgaaatttca gctgaatcac ttttgtactg aacactattg
    12421 ataataattt gatttaaaat aaaaactatc cactttttat cagtatatac actatactcc
    12481 aaatcctgta tattaatatg aatattattt tgtataagaa gcaatttatt ctttgctaat
    12541 acttcttcta caattgactt taatgttatt tcagaaatta aataatcttt ttcaacatgg
    12601 ccaaggcgag catagtacaa tgcacgctca atatgacgtt ctatttcttc catttgaaaa
    12661 cgaatttttc gtgtatgttc atttttataa ttttcacata taagttctga tgttgtaaca
    12721 ggatgtttta tctcatgtac ccattgttca atgaattgtc tatattcgta catttcacga
    12781 tgtatttgag aaacatgatc tgtcatagat ttcaatgata atctaataat attgtagtat
    12841 tcacgttcta tgttagaagt aggtttttca atcatttcaa taaacagata ttttttatcc
    12901 aattcgtcta caatgcgacg taacttaata atttcttttt tctgtttttg ataacaaaaa
    12961 catatccaac aaaataaaat tgcgacccaa gcggctataa gaagagtcaa ctctccttga
    13021 ccaatcccta taagatgaag atatagcact aatgaaattg cacaacaaat atttaagagt
    13081 ataactaata ctttatcttg aagaaaatta caaaacctca tatcctatat cccatccccc
    13141 gttttgtaat gattaagtca gtaataccta taccccttaa cttttctcta agccttgtaa
    13201 tgtttacact taaagcatta tcatcaatat gaatatgatt gtcccataaa tattctacca
    13261 aatctatacg aggcacaata ctccctacat tctgaaacaa ataatacatg atttttagtt
    13321 cagttcttga aagttctata acagcatttt ggtattctac tgtagctgaa ataaggttga
    13381 gtataatctc tctatattta attttctgtt tatctgatga atctactctt ttaagtaggt
    13441 tctttaccct tgctaaaagc aaaggtaagt tatatggttt tgtaatatag tcatctcccc
    13501 ccatagtcaa accatttagt tcatcaattg cactatctct tgctgtaata aaaataatag
    13561 gtatagtaga acaattccta atctgtctac ataaagaaaa accatctata ttaggcaaat
    13621 tgatatccaa gagaattaag tcaggtgttg attcttggat tacttgaagt acatcacaat
    13681 tatttgtatc caatgtttgt gtactatagc cattattttc aaagaattgc attaattcct
    13741 tacagatgaa cacatcatct tcaattatca aaatattggt taaaataata aatacctcat
    13801 ttctttagtt tatattgctt taattttgta agtaatatat ttttccatat attacttaca
    13861 aaattacttt ttaattattc atcttttcca gctactgata gctctttaat aacaactgaa
    13921 ggtgaaccta tactactcat tggaaattct aaatcttccc ctataacttc aatatctttc
    13981 aacaggtcaa aatagtttcc tgctactgta atttgctcca ctggaaatac tttttttcct
    14041 tctttgatat aaaagccttt ggcagctaaa gaaaagtctc ctgttactga atttgcacct
    14101 gaatgaagcc ctgcaaagct agtaaccatc aatccttcat ctatatcctt tataatttca
    14161 tctaaagatt tatctccttt atctatataa aaattagttg gatatatgct tataggtgat
    14221 gaatatgaag attttattcc atttcctgta gtctttacac ctgccttatt agccgttttt
    14281 aagttatgaa gtaaagttat aagctttcca tttaaaacaa cttctttttt aaatgtagct
    14341 actccttcat catcaaaagg agtagatgcc ataccatttt ctaaaagtgg gtcatcaact
    14401 attgtaacta taggagatgc tatcatgtct ccttctctat ttttcaataa agataatcct
    14461 ttttgagctg aatctgcatt aaatatccca gaaaatgtgc taagcaaaga taccatagct
    14521 tcattaagca atatagtttt atatttacct gatggaatac tcttaccacc tactttagat
    14581 aaagcattat caacaccaag ttttgatatc tcacaaggtt ttatttcttc aatagaattt
    14641 gctctattat atcctattcc atcatatttt tgtccattgt cttctatgat aggaactaca
    14701 aaccctataa gcatattagt tttattgctt aaattaagac ctttagtatt agatatacca
    14761 ttacttgata cagaataaga tatagtacat ttagataaat ttaccacctt atctgaataa
    14821 gctttagtct ctctttccat ttctaaagct aaatcaatca acttatctgc ctctaaattt
    14881 tctagctctt tagaataagt ctttacatca ttatagtgct tatctccttc atatataaat
    14941 tgtacatctt cattttcaat agtgtttact ccatctttta cattttttat caacatatca
    15001 acagctttat catctaaaat ttctgtataa gaataaccta tctttccatt taccttacct
    15061 ctaaaagata aaccaaaaga tttatcaaga ttgtatttct caacttctcc ttcatatata
    15121 tttatactta aattttctcc tgtataataa tatatttcac actcttcaaa accttcactt
    15181 agagcctttt taaataatat atctttaaaa tttttaagtt ccacaattat ccctcctatc
    15241 ttccacctac agtaatttct tttaccctta tcataggttg gcctacatta gttggtatac
    15301 ttcctgatga tgagccacac atcccctgag cttgttttac gttattccca accatatcta
    15361 tattcataag tatatcgcta ccttttccaa ttaaactagc acctctcact ggctcttgta
    15421 tttctccatt ttttattaag taaccttctg aaactgcaaa attaaactct cctgttacag
    15481 gatttacaga accaccaccc atttttttag cataaagtcc atctggtatt gactttataa
    15541 tgtcttctgg tctatcttct ccagcagcaa tataagtgtt agtcatcctt gaagttggtg
    15601 caaatttgta gttctgtcta cgagaacttc ctgtaggatt cattcccatt cgtctaccat
    15661 taagtttatc tatcatataa gacttcaaaa ttccattttc aatcagtata ttcttttgag
    15721 ttggatttcc ttcatcatct atattcatag aaccccagta gtttggtatt gtaccatcat
    15781 ctatagctgt aatctttgtg gaagcaatct gttgacctaa tttatctgca aatacagagt
    15841 tcccctttgc aactgatgta gcttccaaag aatgtccaca agcttcatga aatattactc
    15901 caccaaatcc attatctatt gctaccatca tattacctgc aggacaattt ttagcatgca
    15961 acatagtatg cgctactcta gctgattctt ttgcataata ttctgggtct atttcttcaa
    16021 atatttcaaa ccctttacaa cctccaggac cttcaaatcc agtttgattt tcattaccct
    16081 tagatgcaat tgaacttatc cctagtctgg ttcttattct tctatcctca acatatatac
    16141 cttcagtgtt tgcaattaat attttttggt ctttatccaa atagctaaca cttacttgag
    16201 atatatcatt actaaattct tttgcactct tatatgcaac cttcataaca cttactttat
    16261 ctctatttcc tattgaattt ggataaattt ttatattatg tatgttattt attttaatac
    16321 tgtcatttaa tattacactt tttccatctt ccaatttacc taaagcaaca gcagctttgt
    16381 atgctacatc caacagacta tttaatgaat tgtcacttgt ataagcatat atactcttca
    16441 atcctttaaa tattcttatt cctataccat aatttcttcc acctattgca ttttctactt
    16501 tattatcaat taaactaata gaattattta tagtgtcttc ttcaaaaatc tcagcaaaat
    16561 ctcctccagt aattagacac ttttctaata ccttagaagc tatatttttt gaaagcataa
    16621 atttacctcc tactttaaat ttttaattaa cttttattca atctttttac ttgttaacaa
    16681 acattatata ctatattact taaaataagg taattatgaa tatattatac atatgtttta
    16741 tactaaaatg ttatcacatt ttatctatac atacccatat aactagataa ttaaaatgta
    16801 taatattatt tgctatagaa aataatacac tcatacataa taaattttag gagtgaagta
    16861 tatgaatcaa gaatatgtaa aaggaaatcc tatttttact aatattaata aagttccaag
    16921 acaatttcca taccttactg atgatattga tactgatgtt ataattgttg gtggtggcgt
    16981 aactggatgt atttgtgctt actatctagc taaaaacaat ataaagtctg tcattcttga
    17041 aaaaggaagg attgcacatg gaagcactag cgtgacaact tctctattac agtatgaatt
    17101 agatgacaat ttgatagatt taacagaggt tatgactcta aaagatgctc taaaagcata
    17161 taacctttgt acttcagcac ttgaagaatt agatacattt ataaagctat atggaaataa
    17221 gtgtgattat gccaaaagag atactctact ttatactgcc aacaaacttg aaattaaggc
    17281 tatcaaagaa gaatataact taagaaaaga aaatggattt gatgttgaat atattgatga
    17341 atccacaaac ccttttagct ttgatttaaa atcaggattg atagcaaaga atggaggacg
    17401 agaattagac ccatataaat atagtcacca tttgattgat gtaagtttaa aaaatggtct
    17461 tcaagtatat gaaaatacag aagtaaaaaa agtagattta tcaaatgata aggttactgc
    17521 tgaagtatcg tatggataca aagttcatgg taaaaaatta attgtagcta cagggtacaa
    17581 tacaagttta tttacaaaaa gaaattttgc aactaagtca aatactttta atatagcaac
    17641 taaaccactt aaaaacatag cttcttggaa aaacaacatt ttaattagag ataactgtga
    17701 cccatataat tatctaagaa ctactaaaga caatcgatta attataggtg gtgaagatgt
    17761 aagttttgat gatatagaaa atgaaacttt agccaatgaa aagtatgata ttttagaaca
    17821 aagattgaaa agtatgttta aggatataaa agacattgaa gttgaatata aatattgtgg
    17881 ttgttttgct tcaactttag ataatttagg ttttattggt ccagataata aaaataacaa
    17941 tctatggtat tgcttaggtt atggagcaaa tggtatactc tttgcaatat taggaggtat
    18001 aatgttaagt gaattatatc ttggcaaaca aaataaaaat atgaaattat ttaaagtaga
    18061 tagatttgat aaatagatat tgtatattaa aagactataa aaagatgaaa ctttataatt
    18121 ctaaaagtta actttgatac agtcttttat aatgttcctt ttaaaaatac tttatttata
    18181 attaaatata aagtaaaatg ttaaaatata actgataaaa tttagtcata agataatatt
    18241 taagtttatt tgatgaaata tggaggaatt attatgaaag aaatactcgt tattgctccc
    18301 actaaaggaa cctatgaaaa atcaattcac atagttaaaa aaaacaaata tacaaatatt
    18361 gatgttgtat ttggcaatct aaaagaaggt atacctcttg ctgaaaaaag tattaatcat
    18421 ggtactagaa ttatcataag cagaggtgga acttacaata tgttaaaagc tacttacaat
    18481 atacctatag ttgaaattaa agtagatgct tatgatatta taaaaagtta caaagaggta
    18541 aaaaattcca acgaaccatt tggaataatt ggatttaata atgtcatcta cggtttcgat
    18601 attatagaag aaatattgaa taagaaaata actatgattg aaattgaaaa agaagaagaa
    18661 atttatgatg ctatagaaaa gtacagaaag aaaggtataa acacatatat aggagatacc
    18721 actgttgcac atattgttaa gagacttaat tgtaagggaa tattaatcga atctagagaa
    18781 gaaaacatac ttagagccat tcaacaagca gaacaaatac tcgaagcaac caaggatgaa
    18841 caaaaaagaa gattacagat agaagctatg acagattttg tacatgatgg aataataaca
    18901 gttgataaaa attttaaaat aacattattt aataagagtg ctgaaaaaat atttggtata
    18961 aaaaaagaaa atgcacttta tcataatgtt gtagatgtta tcccaaacac agttcttcca
    19021 caagtagttg agactatgga acctcaaatt ggtgaaatac aaaatatagg gagtacaaaa
    19081 attattacaa atagagttcc aataattgtt gatggccaga ttcaaggggc tgtttcaaca
    19141 tttcaagata gcaaggagat aagttctttt gaacatacaa taagaaggag cttattaagc
    19201 aaaggtctat ctgctaaata cacttttgat aatataattc atgctagtga aaagacaaaa
    19261 gattgtatag aacttgcaaa aaaatatgct tgttatgata ctcctatgat tattactgga
    19321 gaatctggag ttggtaaaga attattttgt caaagtatac ataactatag taagcgtaaa
    19381 aatgcacctt ttgttgctgt aaattgtgct gccatacctc catctctaat agagagtgag
    19441 ttttttggtt atgaggaagg ttcatttaca ggtgctagaa aaaaaggtaa agcaggtgta
    19501 ttcgaactag cacatgaagg tacaatcttc ctagacgaaa taagtgaaat ccctcttgaa
    19561 cttcaaggtc gattattgag ggtattgcag gaaaaacaag ttatgagaat tggtggtgat
    19621 aaagttattc ctatagatgt aaaaattatc tgtgcttcaa ataaagactt aaaaaatatg
    19681 atgcgcaatg gtttatttag acaagacctt tattttagaa taaatatact aacattatat
    19741 cttccttcat taaaagaaag aaaagaagat attataaaat tatcagaatt ttttatacat
    19801 aaatattcaa gcaaatacaa taaaacgcct ttaattatca ctccaaatat taaaaaggca
    19861 cttcttgaac gagaatatga aggtaatata agggaattgg aaggtatgat ggaaagggct
    19921 gttattgtgg aatcatttga ctcgatttta ttagaaaagg ttagcaatga atatacaaat
    19981 aatatagttg ataaagataa tagtgatttt gaatgcagta atttagattt aaaaacttta
    20041 gaaaaaaaat atatctataa aatttataac gaaaataata gaaatactac aaaaacctgt
    20101 gaaattctaa agataactcg ctccactcta tggcgtaagt taaaagaaat agaagatgat
    20161 gtttaaaaat aatacatacc tatatatatc aaaacgtttt atgtttaatt ttaatacata
    20221 aggcgtttat ttgttttcaa taaaaaatac aattatatga taaaaagaat ctttgtaagt
    20281 gaaattctca tcaatcatac ctttaattat aagtaatcta cagcatttca atcgattatt
    20341 aaaaataatc atgataacta aaaatacaaa ctctatatat tggcatagta attgctttta
    20401 taatacctat ataaaaatga aagggagtga tactagatgc acatcttaaa atcagtgaaa
    20461 aagataccag gaggacttat ggtctgtccc ttgttattgg gagcactatt taatacagta
    20521 tgtccacaag ctcttgaaat tggaggattc acaacatcct tatttaaaac tggtgcaatg
    20581 agtatacttg ctctattttg tctatgcaat ggagctcaaa tcaatatcag gcaagctggt
    20641 aagccattga ctaaaggtat agtacttaca gcaacaaaat ttataattgg tgcagtatta
    20701 ggtattattg ttagtaaatt tattggacct aaaggtataa ttggtattac acctcttgcc
    20761 atagttgcta ccatgactaa ttcaaatgga ggtctatttg ctgctcttgc aggtgaatat
    20821 ggtgactcca cagatgttgg agcaatatct atcttatctc taaatgatgg tccttttttt
    20881 acaatgcttg cttttggagt aactggactt gcaaatgtac ctatagtagc actatttgca
    20941 gcacttatac caatactact tggatttata cttggtaacc ttgatgaaga tatacgagaa
    21001 tttcttgcac ctggtacaac attattaatt cctttcttcg cttttccttt aggagcagca
    21061 ttaaacttta atcaaattat aactgctgga ctacctggag ttgtacttgg tttaggagtt
    21121 actataataa caggtatggg tggctacttt gtaatgaaac ttatacgtgc agaacatcca
    21181 gcagtaggtg cagctacagg tacaacagcc ggaaatgcag ctggtactcc tgaagcttta
    21241 gcagctatag accctactct agctacaata gcagctgttt caacagtaca ggttgctgct
    21301 gctattattg ttacagcaat atgttgtcca atgttagttt cctatctaga taaaagagaa
    21361 aaaaagaaaa aatctatttt taaaacaaat aaaaatattg aagtaaatta atacttaagg
    21421 aggtaaaatt aatgaatgca attattcatg caaatgaaaa agataatcta gttacatgtg
    21481 tcagaccttt agttaaaggt gaaaaagttt caattgatgg aaagtcttac atagtaaatg
    21541 ataatatacc tgtctttcat aagatggcta cagaagatgt aaaaaaaggt gatgttgtat
    21601 acaaatacgg tgaaattata ggaattgcaa cagttgacat aaaaactggt gactatgttc
    21661 atgtacataa cattgaaagt acacgtggtc gtggagataa aaaataggag gaagttaaaa
    21721 tgaaattaat gggttattta agagaaaatg gtcagtttgg tattcgtaat catgttttaa
    21781 ttattccaac atctgtatgt tcaagtgaga cagctacaag aatagcatca cttgtgcctg
    21841 gagcaatagc aatccctcat caacatggat gttgtcaaat tggttctgat atagagctta
    21901 cagcaaagac attaatagga tttggaaaaa atcctaatgt tgccgcagtt ttagtagttg
    21961 gtcttggatg tgatggtatt caagcaaaag aattagcatc tgaaatagct acaacaggaa
    22021 aaaaggtaga ttacgttgtt atacaagaat gtggtggaac attaaaaaca gtttctaagg
    22081 gaactgaaat agtaagtaaa atggctcgtg aagtatcaaa agaagtaaga gttgaatttg
    22141 gtatgagtga aattacatta gcacttgaat gtggtggttc tgacccaaca agtggaatag
    22201 cttcaaaccc ttcaataggc gttgcatcaa accttcttgt agatgaaggt ggaagtagca
    22261 tactaagtga gactacagaa gtaattggtg cagaacattt gttggctacc cgttttgaag
    22321 atgaagaaat gaaagataag tttcttaaat ttgtaagtga tgtagaaaaa agagcaattg
    22381 ctatgggaga agatttacgt agtggtcaac ctactccagg taataaagct ggtggacttt
    22441 caacaattga agaaaaatca cttgggtgta tgtataaggc aggcaataag ccattcaaag
    22501 gtgctcttga atatgctgat attgtacctc ctgataaaaa aggattatat tttatggata
    22561 ctccaggtca agatatagat tctatcactg gtatggtagc aggtggtgca caaattgtaa
    22621 tcttttcaac tggtagaggt actccaacag gaagccctat ttcaccagtt attaaaatca
    22681 ctgggaatag tgatacatac aacaaaatgc cagacaatat cgatataaat gcaggtagaa
    22741 ttataacaga tggtgctaaa atagcagata taggtcaaga aatatttaat gaaataatag
    22801 aagtctgtaa tggtaaacat accaaagctg aaagtcttgg tcatagagaa tttggcattt
    22861 acagaatttc atctacattt tagattgtaa caaaatatct tttataaata taatacttat
    22921 gtagatacat aagctaaaag ttctttctaa tcctataaaa attttctata ctgaaaagga
    22981 actgtatcaa aattattttg aaaacagttc cttttttaac ctcttttact caaaatatta
    23041 taaaataaga tttaaacaat aaacgtgtct tgattaatct ctaagaagac ataaataaag
    23101 ctaaatgtaa agtgagataa ataaaattta atatctttgt atgaaaagat atttggtata
    23161 atatgtctga gagaattctt atttgtaaac aaataaagga tggaggatga aacaaaagaa
    23221 tgaactttga aaacacaaaa aataaaaaaa taatagctac tttactattg acactccttt
    23281 taatcgtgtc attaacatgt gtcaatttta ttatgaatct aaataaagta acacataatg
    23341 aaacagaacg atatcttgta gaagtttcac aaagtatttc cactacaatt aatgtgaaat
    23401 gtaaaagtat atttaggaca ctatattcta tgggagatac ttattttgag attaatgata
    23461 gtgaaaaaga agtcaataga tacttatcaa aaaaattaaa attatttgaa tttgattggt
    23521 taggtattat agattctaat ggaaattttg taagtgccct taaagataca ggtaatgtta
    23581 aaggacaaga cttttataga aaagcccttc aaggtaaaga aacaattact cctgttaaat
    23641 cacaatctgg tacatatgga attttatatt ctgtaccact atacaatggc gataaaattg
    23701 ttggagctgt tgctgcttgg aatactattg atactatgag aaatttatta agtgtagaaa
    23761 gctttcatgg tgaaggattt tcccaaatta tcgaccaaga tggaaatttt attgtaagtt
    23821 cagcaaacaa aaatgctcca aaaaatgtgg taaatttttt tgacatgatt aaacaccaag
    23881 gaaaacaggt tgataaaaat aaacttgaag aaatgaaaat aaacttaaaa aagaacaaaa
    23941 ctggttctat gtattataca ttaaatgatg gtatttctaa ggcaatgcat tatcttccat
    24001 tacaagaaga aaacttatac ctcctttcag ttgttccaac aaaaacagca agtgcacagt
    24061 ttgatttact tctaaaaaaa gcaatcttca tcaatatttt aattgttata ctatttttat
    24121 tgcttattat attgattatg tttataaatc aaaagaacag ggcacaactt ataaaaatcg
    24181 cctatgtaga ctctgtaact aaaggttaca gtaaagcacg ttttgatatt gaagctaaaa
    24241 aaattataca gtcatcttca tcagaaagct attcattggt atctataaat attcagaaat
    24301 tcaaacttat taatgattct tttggtagtg aagcaggaga taaaacatta aagtacatac
    24361 atgatgtcat tagtaattat ctagaaccag gagaattgct ttgtcgagtt tctaatgacc
    24421 agtttaatgt acttattaaa actattcctc aaaaagaaat tcttaagtac atagaaaata
    24481 tcaccaatga tattaatagt ttcaataaaa atgaagaatg taaatatttt atagctctat
    24541 ctataggtgt atataaaatt gatgacccaa atctaccttt agtttcaatt caagaccgtg
    24601 caaatgttgc acgaaaaaat aataaaggaa acattggaaa tcatccactt tatacatgtg
    24661 ttttttatag tgatttagag cgtttaaaaa tgattaaaga aaaagaaatg gaaaatagaa
    24721 tggaggaagc attaaataat aacgaattta tagtatatct tcagcctaaa ataagcctca
    24781 aagataatac tattgtaggt gcagaagcat tagtaagatg gcaagaccca aataaaggcc
    24841 ttataccacc agacgagttt attcctttct ttgaaaaaaa tggttttatt acaaaactcg
    24901 accactatgt ttttgaaaaa gtatgtagta tgataagaaa atggatagat aatggagaaa
    24961 ctccaatgcc tgtttcagtt aatttttcac gtgctaattt aaataatata gattttttag
    25021 aaaaatacag aatgcttcgt aataaataca acattcctgc taagctctta gaaattgaat
    25081 taactgaaac acttgtattt gaaaatctcc aaaaattaat taatataata gaccaaatcc
    25141 atgaagaagg ttttcaatgt tcactagatg actttggaag tggatactct tcactcaata
    25201 tgttaaaaga aataaaagtt gacacattaa aacttgaccg tgcttttttt agctcaccta
    25261 atgcagataa tattgctgaa aatcatgtaa tagaatctat agttaatttg gcaaaaaaat
    25321 taaatatgaa tagtatatca gaaggtgttg aaacaatctt gcaaatggat tatcttaaaa
    25381 aaattaattg tgatatgata caaggatatg ttttttcaaa acctctgcct caagaaatat
    25441 ttgagaaaat gacttttgga aaaaaactag aagaaaccag tatggatagt tccgtttaaa
    25501 taaaataaaa aacttgtctc tataaaataa ttttaatcat tttataaaga caagtctttt
    25561 tattttccaa tttaatttta ttttctaaat ttcttatact cggcttgttt taaattccct
    25621 ttttaagaat aataatacga ttatcatagc aataaaatct gcaactggct gtgccaacca
    25681 aacaccatct aaattaaatt gaattggaag tacaatgatt aatggtataa ataaaataaa
    25741 ttgtcttaaa agagttaaaa acattgaatg tttaacttta cttatacact gaaaatacac
    25801 aggtcctaca atagaaatcc ccattatagg caatgtaatt agatttatgc ttatacctct
    25861 tactgctata tccatcaaat ctgaatcttt attaaatata cctacaaata cttctgggaa
    25921 aacttgtact attaaaaatc caaatgacaa tatgacaact gacactatga tagataatat
    25981 taaagttttc tttgcacgtt catattgttt tgcaccataa ttgtatgata ttatagtttg
    26041 cataccttga ttcaatccaa acacaggcat cataaacatc aataaaatag atgtaagtgc
    26101 tgtcatagcc ccaatagcta aatctccacc ataaaccttt agagaattgt ttgtaaacat
    26161 gtgagttata cttatagcta cttccatagc aaaaggtgtc attcctattg caaatacctt
    26221 ctttactata tctttattca attttaagtt acattttttt agttttaaag tggacttccc
    26281 tcttataaaa taatatgtta cccaaaagaa tactactatt tgacatagta ctgttgctat
    26341 agctgctcct tttataccta aattaaacac aaatataaat atagggtcca atatcaggtt
    26401 taatacacat cctacaatca ttgtccttgc tgctaatttt ggattacctt ctgctctaat
    26461 tgcattatta agagaaaatg caactaaatt aaatatactt ccaatcaata tgacactcat
    26521 atagtctttt gcataaaata ttgaatcttt acttgcaccc aaaataaaaa gtatatcttc
    26581 taagaaaact aatcctaata tagttataat taaagagata attatagata atgtcaaagc
    26641 attacctaat atcttctctg cttcttctct atttctttcc cctaatttta tagacaatct
    26701 tgtgctagca cctactgata ccaacattcc aaaagctatt attagagtaa atactggcat
    26761 agttacacca agacctgcaa tagctataga gccaatacct tccatagaac caataaaagc
    26821 tctatctact acattataaa gtgaagttac catcattgct attattgccg gtatcgaata
    26881 cctaaataat aaaatccata ttttttcatg tcttaatgct tcttgattct ccatgttgtc
    26941 accatttaca ccatgtcata agttatacaa attggtttat ttgttcttgg gtctgttgct
    27001 atttctgcat caatattaaa taattctttt agattatcct tcgtcataac atcatatgca
    27061 tcaccttcac aaactatttt acctttctta atacctatca tgtggtcagc aaatcttgct
    27121 gcattattaa gttcatgtat taccatgaca atagttcttc cttgtttttt atttagttca
    27181 tctaataatt ttagtatctc taattgatgt gccaaatcta aatatgttgt aggctcatcc
    27241 agtaataata tatcagtttc ttgtgccaaa gccatagcta tccatgccct ttgtctttgt
    27301 cctcctgata aatcttccat atttctatcc ttaaattctg caattccagt aacttctaaa
    27361 ctccaattta caatctctct gtcttcttta ctagaattac caaatccact tttatgagga
    27421 aatcttccat aagaaattaa ttctgaaacg gtaagaccac taggagcttg agggctttgt
    27481 ggaagtacag ccatatcctt tgctatttct ttagacttct ttttgtagat gtcctctcca
    27541 tttatatata ttttcccact attagggcta ataattctac ctatagtttt aagtattgtc
    27601 gattttccac aaccattagc ccctatgatg gtagttattt ttccttttgg tatatttaaa
    27661 tttaaatttt ttataatatc tgtgtttcca tatgatacat ttagattctt agtatttata
    27721 cagttcataa caacaccctt tcctaatcat ttaccaacat taaataaata aagtacggca
    27781 ctcctattat agaaactact atacctactg gtatttctat aggtgctaga agatttcttg
    27841 atatagtatc tgctattaaa agtaataatg ttcctatcaa tgctgatact ggtattaatc
    27901 ttttatgctt tggtcctatt atttttctac ctatatgtgg agctattaaa cctaaaaatg
    27961 aaatactacc tgctactgct gtcgatacac cagataaaat tactgcatat attataagtc
    28021 ttcttctttg tttttctaca tctacaccaa gacttataga tacttcatca cccaaattta
    28081 aaacatctat atatttagat ttataaatag ttatacccat aaaaatcact ataaatggaa
    28141 gcacagctat tacatatttc caactggttc cccaaatgct tcctgatgtc catgccataa
    28201 ccctattaaa ttcttgtgtt gtaaatttca attggaatat cgtcaaaatt gaagtaaaag
    28261 ctatgtttac acctatacca ataagtagta atcttgacga atttatacca cctttccatg
    28321 caagagaata tataagaaat gctccaaata aagctcctat aagtgcaaca ataggcattg
    28381 taaatacagt caagttactc ataccttcat atacattacc attcataagg tatatataaa
    28441 ctactacaaa caatgcagca cctgaattta ttcccaaaat tccagaatca gctaaatcat
    28501 tctttgtaac tccttgaagt attacacctg ctgttgataa tgcagaacca accaaaagtg
    28561 ctattactat tcttggtaat cttaatttaa atattgcaat ttcatgatta cgctgaccct
    28621 gccctaaaaa agtctttata acatccattg gttctataga aaatgagccc atatttaaac
    28681 tcaataaaaa agtgataaaa ataagcacca ttaaaattga tataacaagt aatgttttag
    28741 aatttatttt tataatattt ttcatagtac ctatttctcc ccctttctaa caaggtatat
    28801 aaatattggc actcctataa gtgctgttat agaacctact ggtgtctcat atggaggatt
    28861 tatcatccta cttaatatat ctgtatatac aagcaataca gctcctaata ctaaagagct
    28921 cggtattata tacttataat ctgaacctac tattccttta gctatttgag gaactataag
    28981 acctataaaa ataatattac cagacactga cacagatgct ccagtcatta atattacaag
    29041 tactatacat ataaatctaa caaagttagt tttttgtcct aatcctattg ccacttcttc
    29101 tccaagactt aatatagtta ttcttggagc cataataata gctaaaataa atccaatacc
    29161 accagctaca aatagcaatt ttataccttc ccacttcact cctacaagcc ctcctgatat
    29221 ccagaaactt aattgttgag ataggttaaa atacatagat attgctgatg ccaaagaaat
    29281 tagcaatgta ccaagagcga ctcctgcaag agctaatttt acattattaa ctcttcctct
    29341 tgatttagaa cttataaaat atataaataa tccacttata cttgctccaa taaatgcaaa
    29401 catcattaca ctaaaacttc catgtataag ttgaggaagt tttttttgaa gtaccagtga
    29461 tatcgaaatt gcgaatgtag ctccttgtgt aattcctata acagatggtt cagctatagg
    29521 atttctcgtt attccttgca ttattgcccc agaaactgct aaaaaccctc caacaagtgc
    29581 agctgctatt gctcttggaa gtctaacatc ctttactatt ctcatattta ttccatcttc
    29641 catttttatc aaactatcta tgacagttga tagattcata tcttttgccc caatagttat
    29701 ggataataca attccaaata caagtacaac tatacttact aatatcaaca aataagttat
    29761 atttttttta ctcagcttag tcaattatcc tacctccttt tcccttttaa tcgtcagtat
    29821 aatatcatct attaagtatt ttaattttaa gatttattaa aaataggtgt gaaaaatgaa
    29881 tatcattatc tcacacctac taagttaaga attactttgt taattcattt ttaactgatt
    29941 ctaataataa ctcttttcca attggattgt atgattgact gaaaaatgga gcagcatcaa
    30001 gtattgttac attaccatct tttactgctc ttatttgaga ccagatagca ctattttcta
    30061 aatctttttt atctgcttct gtagcaatta caactatatg gtctgcatct atatctgtta
    30121 aaccttccat acttaccatt ggtagtgtta ttccatcctg ttttggcata ttcttaggtc
    30181 tagctaattt catatcatca ttgattagag tacctattcc accatcacta aataccataa
    30241 attgacctga acttgccaac actggtaaat aagttttttc tccattcttt tctataactt
    30301 ccttacctaa ctttgtagct ttttcatcat acttctgtag ccaactttta gcttcttctt
    30361 ctttgtcaaa taactttgaa acatcagtta acttgcttct ccagtcattt gcataatctt
    30421 tcatcataac tacaggagct atttctttta attggtcata tattttttct tgtctttgac
    30481 tcattattat taaatcagga tttacttcta atatagcttc catatccata gtatccatca
    30541 ttgagtgccc aactatcttt acacctttaa gctcttctct tatgtaactt ggtaatttat
    30601 cagtttcata tgagtctaca tttgctgttg caacaggctt ataacctgct aataatagtt
    30661 cttcactact tcctgatata tcaactattt ttttaggatt tgatggtatc ttaacctctc
    30721 ctttaacaga ttgtacaact cttgtctctg atttatcttt atcctctgta ttcttatctg
    30781 aacatgctgt aacaagtact aacgtagtta taatagatat aaatattgca agtgatttta
    30841 tttttttcat gattacttct cctacttgtt tattatactt acatgatatt gattatcata
    30901 agcacatatt tattatagca tttttctatt tttagtcaat attattttgt aaaaggataa
    30961 aaattcctaa ttaatcttaa ttatatatag cttagataag gttttcttat attaaacagt
    31021 atttttttta agaattggac acaaacacaa tgacttttag gttaaacaat gataaaatgc
    31081 aatttataga taatgaattt agtaaggagg attcaaaaca tggtacattt agtatattgt
    31141 gataatgcag gaaaaaaagg agaaaaggtc ttagataaaa tcttggctgg aacaaaaaca
    31201 atggttatta gaggtgctgc tggtagaaaa ataccacata gtagagtatt tgaagatgag
    31261 ttactttatt ttatggaaaa aggaactgga aaaatatctg caaaagccac tgttaaaaaa
    31321 gtagaaaact atgttaaatt aacagaagat gaaatttcta aaattttaaa tgaaaatcaa
    31381 ccaaaattaa atttatctga aaagcaaaaa gttcgttggc ataaaaaatg tttatgctta
    31441 gttgaatttg aaaatgttga agaaataaca cctcttgatt ttgaccatca aggaaatatg
    31501 gatgattggc ttattgttga aaaaattgaa gatgtagttg ttggtactag tataccttac
    31561 aattatgaga agtctaaatt ttaaagtatc aataacataa tactatattg ctaaactcta
    31621 cttaaataaa aaaagagtgc atttaataat tataaaaatt atttttatac actctttttt
    31681 aataataaat ttaaattact tactttctaa tctttcatat acatctataa attcttctgc
    31741 taattgttga aaattcatag ctgtcattaa atggtcttgt gcatgaataa gtataacact
    31801 tatttcagtt ttattaccat tagcttcatt ttgtatcaat tctgtttgga atttatgagc
    31861 tttatgaatt gattctttcg acttttcaat acattctcta gctttatcta tattcccgct
    31921 ctttgcttct tttattgctt caaatgctag ccctttcgca tcacctgcaa atccaattat
    31981 tccaaaagat atctctatca tcttctcatc catttattcg acctcctaaa tatctctata
    32041 gcttgatatg actaatccat tcttttctaa aaattctttg acttttttag atgtaagtat
    32101 cttgtgctct ttcgttctat caatagcata agatgtagaa tttaaaatat aatcatctaa
    32161 aaaagctggg tgagacataa tatccactac atcatacttc attatttcgt ctatattttt
    32221 tataaaatat tcttcactta cattttcctt ataaaaacta tctattaaag gaactacttt
    32281 agaatactct aaattatatt caaatccacc tcttattgga agattatact tggttacaat
    32341 cttctctact actggcttta aacttactaa agtatggata tgatggtgac tatctaaatg
    32401 agatatatct attccaagcc cttttaccct atctatttga gcacaaagtt ctctataaat
    32461 ctcatcacaa tccatttttt caataatttc gtctgtaatc ctcctaataa aaaaaccgtc
    32521 tttatcaaca atagttttta agttagataa taatggtcta ccacaactta aagtcatatg
    32581 aactccacaa tttaaagttt tattttcttt aagtaagcct actccatgtt ctaccccagg
    32641 catatttgcc atcatagaag tagacctaac tataccatta ttatgagctg aaattattcc
    32701 atagttcaca gcttcacaat atccaaaatc atctgcattt attataattt tagtcatacc
    32761 ataacctact ttctatagac aactatctat taaatctagg aagataatct ttatgagctt
    32821 ccaacatttc gtctaatata gtttttgcta agtcatcaga tggaatcaat gggtttatac
    32881 aaagtgccaa aagagcagat tcataatcgc catatacagc tgctttagct accaatactt
    32941 caaatgattt gatttggctt actaatccat gcacagaatc tggtaaatat cctatactta
    33001 ttggttttgg accatttttg gttattacac tactcacttc taccgcttgt tcatctttaa
    33061 aatctcttat agaaccattg tttaatgtat taactacttg gatatctttt ttgtcattgt
    33121 atatagaact tattaaatta caagcagcat cactatagta agcccctcct cttttttcaa
    33181 gttgaggtgg ttttacatct aatttttcat ccttatataa ttcaaataat tgttcttcca
    33241 attctttaac tacttgacct ctagcttttc cctctttaaa ctctctcaac tcatcctcaa
    33301 gcatttcttt agttttatag tagtatctat gatatggaca aggtattgca cccaaagatt
    33361 ttacaaattc agcattaaaa tcaattgcct ttatattttg catacttata tctgctttga
    33421 caaacttatc aattgcttct tttgtaacat cttctccatc taaagataca tttagaccat
    33481 aaaccatatg attaagacct gcaaaatcca tacttattct gtcactttct acattaaata
    33541 gtttggcaac atcatttttt aaatgaattg gcacattgca tagacctata tatcttctaa
    33601 aattagtata tttgaataca gcctctgtta ttattcctgt tggatttgta aaattcacca
    33661 tccatgcatt tggacataat tcttccacat cttttattat atcaaatata actggtattg
    33721 tgcgaagggc tttaaataaa cctccagcac cattagtttc ctgccccata actccatgag
    33781 ataaaggaat agtttcatct tttattctcg catctaaaag acctactcta agttgtgttg
    33841 tcacaaagtc tgcatctctt agagcttctc ttctatctaa agtaagattt attttcatat
    33901 ctactccagc tttttttacc atcctcttag ctagatttcc aactatctct aatttttctt
    33961 ttccagcttc tatatctaca agccataatt cctttacagg taactcttca tatctattta
    34021 taaaaccctc tacaagctca ggagtataac ttgaacctcc tccaattgta actattttta
    34081 gtcccttttt catctcatta taccatcctt acctataatt taatatactc tacaaaaccc
    34141 tatacattta tttaccgaaa taaaactacc tagttgttaa ttttcaattt attagcttcc
    34201 attttttcag acgctatgac aaatggaata tatattaata ttgataatat taaattaaat
    34261 agtgctaaag ctccaccagt ccagtcacct gtagccatcg ctccacctaa tataggaggt
    34321 gttacccaag gtattttagg taaaattact ggtgctacta atccaaaatc tattgctaag
    34381 tatgatacag cactacatat cactggtgca agtacaaatg gaatcataaa tataggattt
    34441 aaaactattg gaagtccgaa tatcaatggt tctcctatat taaatattgc aggtggccca
    34501 cctaatacta tcatttcttt acgtcttctc ccagctatta ataaagctac tattaatccc
    34561 aataccattc cagaaccacc taaatataca aatgcatcaa agaatgcacc tgcaagtaca
    34621 tggtatcctt ccgttgctcc tgcttgagcc aaggcagcat tttctccacc taatttcata
    34681 agtatagttt ctgtaaaagg aagtgctata tttgcaccat gaagtccaac agtccaaagt
    34741 atatgaatta taaatgcaat aagcattgta gaacctagac tatcagctac atttgatagc
    34801 ggtgctccta aataagtatt taaaatatct gttaagaaac ttccattaga aagcattttt
    34861 ataaatattc ctattacagt aaatatcatt atagttaaca ttcctggtat taactttgca
    34921 aatgatcttg aaactgctgg tggaactcca tctggcattt ttatagttaa tttctttacc
    34981 ttagataatc ttacaaatat ctcagttgat aataatccta ttaatataga agagaataaa
    35041 gctgctgtac ctaagtaggt ttgttgtatc attccccatc cttcaactac tgtagttcct
    35101 ccctcaggaa ctattttacc aatttgtgga gccattataa aaaagataga taaagctata
    35161 agcccagatt gtaacccatc actctcataa gattttgcta gaaaataagc aacacctatt
    35221 actaagaata ctgccattgt acctattgct ccccaccaaa ggtcgcctcc aaatgtggtc
    35281 caattttctc caaatatact tgccatcaaa tttttgtatg cttccaatgg taagttattt
    35341 atcaatgttc ccaaggcacc taccatagtt ataggtatca ttactataaa tgcatctctt
    35401 actgctacta agtgcctttg agctcctatt ttagcagcta caggcactat atatttatcc
    35461 ataaatgaca tgaatttttc cattttacat cctccagtac aactttattt tgaactattt
    35521 aactctacag ctctatctaa cacagctttt ccattacatc ttccataatc catcatattt
    35581 ataacttcta ctggaatatt atatggtttt gccatttcag ttgcttttcc taatacatat
    35641 ctaacttgag gccctaaaag taagacatca caattttcta tttcattttt taagttcact
    35701 tctgatatag cccatatttt tgcttctata ccgttttcct ttgctgcatc ctccattttt
    35761 gtaactaata aactagttga cattcctgct gaacaagcta acattatttt tatcattttt
    35821 tcccctccaa attagattca ttaaatattt tttaagtcat aaatttttta aactatacat
    35881 ttaaaatatg cttttctatt aattttttct taatgtatat atcttatttt taaatgtata
    35941 tacatttaat tcttgatatt attatatacc aaatgtatat acatttcaag aaaacttttt
    36001 cattttattt aaattttatt cgacaacagc attcaaagtt aatgtatatc catagtgata
    36061 tgtgtgtgag tactcaaaaa tctcactatt ttgtaactta caaatttggc ttatatataa
    36121 aagtggctct ggttcttcta attttagatt atccatacat tcttcattcg atattacagc
    36181 ttgtacttct ctctctgaca tggctatctt gtatccacac acttcttcaa caaaattata
    36241 aagagaattt tcacaatgag tcttatttaa atttggaaat aacttaactg gcatataagt
    36301 aatcatatat gaaactcttg catcattggc taatcttact ctttttattt cccaaacatt
    36361 ttcttctaaa cttatattca acttacttga taaattttca tctgcctgaa ctatttcaag
    36421 agataccaat atattagata ctttatagcc tttactttcc atctctttag taaatccact
    36481 tacagacgat atattatcat atatattatt tgcaataact atactaccta cacctctttg
    36541 tctagttata tagccttctt taactagatt attaagtgca gaccttacag tcattctagt
    36601 tacattaaat tcttcactca gctgtttttc agaagggatt aaatcccctt ctttcaaact
    36661 gtctgaattt attaactctc taacatgatt ttctataact ttataaattg gctctttcat
    36721 ttaattcact ccttattaat taaatctttt atataataag ataattttat atacaaataa
    36781 agaaaatgtc tatacattta agataattta aattttattc agacatcttg tacaactttc
    36841 cgagttttaa taaagatata aatagactta tcattaatat accttttaat atacttgata
    36901 aggttatact ccaccataca ccatttagac ccaacatttc tggtctagat ataaagtaag
    36961 ccaatggtac tcttgcacca gtcagtatag taattataat agaaggtata taggttctgc
    37021 ctaatccttt gaaagctcct gttgttatta tttctaaaca cataaaaaat tgagaatagc
    37081 ctaatatttt aagataaatt gctccttttt ctattgcctc actttcatct ataaaaatag
    37141 aaaatatact ctctcctgca aatactaata ccaaagtagt tattatccca agtacagaag
    37201 atacagttat agctattttg caacctttat ttattctaga atacttcttg gcaccataat
    37261 tttgtccaac aaaactgcct aatgcaactg atagcccttc agccgtcatc caggaaattg
    37321 cttctatttg tgagcctact ttttgtactg caacagccac aggcccccat gatgctacta
    37381 ttactcctaa tagcattgag aatactgtaa acataccact ttgtattgct ataggtaatc
    37441 ccaatttata cagaacttta taatatttag tctctatttc tttaaatagt cttaatttaa
    37501 aatattcttc tttactttta actattatat atataaaaca tgtagtaact actatctgag
    37561 caaatacagt tgcaatagca gctcctgcaa caccaagttt tggagcaaat ccccatccaa
    37621 aaattaatat aggatcaagt ataatattag ttattaatcc tactgtatta atacgaaaag
    37681 gagttttact atttccaaga ccgttgaaaa ttgcagtaaa tactggatta ataaaataaa
    37741 ataccatacc tagagctact attatcaaat actgtctaga catggttata acttctaagt
    37801 ctcctaatct aaaaaaacct atcaattgtt tattaagtac tattaaagaa attgtatata
    37861 ttattgctag tataatattt atttctattg ctgacttcat atatgattta gtagtgctta
    37921 tattatttgc accaatactt tgtgctactt ttatctctcc acctactttt gaaatcataa
    37981 taaatgccat tgcaagccat ggaaaaaaac cagctgttcc aactgcagct actgctttac
    38041 ttccggcttt accaacccat atcatatcta tcatgttata ccccatctgt ataaatgaag
    38101 tccccattat tggaagtgat agcttaagta atttttctgt aattcttcct tctgttaaat
    38161 taattctgtc tttcattttc cacctctttt atattaaatt tatttgctta tatagctaga
    38221 ttaatcattt tatttgattg agttcactat ccttcaattt atattatcat atctcaagat
    38281 tgcatctcaa ccatacttat gtttaagata aagtatttta cctatctctg ataaaattaa
    38341 tacttatgaa tataaatata gtcatttcat caatacatat tataaccctt aacttaactt
    38401 caattaagta tacatactgt aatcaattag aatattttat aatgtatctt ccattaagaa
    38461 ataacaaata taagtgattt atctataatt ttatttatac ataaaatagt atactgcaat
    38521 aataaaaaaa ctatatttga atactttaag catgtttact aattgtaatt tccacacaaa
    38581 tttgataata tatgaatata ttatggtgaa atttatagat atatattaat tatggggaga
    38641 ttgtgggagg ttcttttcac aagacattta agctatgaaa ataaataaaa aatttaatag
    38701 agttgatatg caggtttcaa tacttactgc aattattgta ataatatctt cctcatgtgt
    38761 atattttttt aattatacat taacttataa gagcatgata tactctttaa gtgagaggtc
    38821 aaaacatatc tatgaatatg tagagagaaa tattgataaa aataccttta ctgaaattaa
    38881 ttctaaagaa gaccaatcaa aaaagtctta taaaagttct aaaatcctat tagaatctgt
    38941 aaaaaatact actggagtca tgtatctata tactgctaaa aaaacaaaag atggatcact
    39001 agtatatgtt gttgatggtt taaattcatc tagaagtgat tttagaaatg ctggtgattt
    39061 gatagaaaaa gagatttggg gagatcttaa caaagcatta gataatcata tattgttacc
    39121 taaaaagatt aaatcaacat cttggggata catatttata tcgtattttc ctattcacaa
    39181 tgatgttggt aaggttgttg gagtaatagg aattgaattt gaagcaaaac atcaatacaa
    39241 tacatttaaa tttataagta tagttacacc aataatagca ttattaactt gtctaatatc
    39301 agcattgatt gcagtgattc tatttaaaag aatatctaat cctacatata ctgattttgc
    39361 taatacagat tatttaacag gcctaaaaaa tagaaatgct tttgaaatag atatgaataa
    39421 cttaaacaat tcaaatctaa agaatctaat atctatcata tcaatagatt tagacggttt
    39481 aaaaaagatt aatgataaat ttggccatca gacaggagac ttatatatta agcaagcaag
    39541 taaaattata caagatgtaa taaataaaaa atatatagtt tatcgtattg gaggtgatga
    39601 atacataatt attctaaaaa atttatctca caaagaaata aatagtatta taaataatat
    39661 taatttaaaa atagttgaag aaaactcaac taatgaatta aaattatcaa tgtctattgg
    39721 atatgctatt tttgatgaaa aagtagatgt ttctttattt gatacatatc gtcgtgcaga
    39781 ttctcaaatg tatgataaca aaaagaaaat taataaataa tatcaataat aaatttaaaa
    39841 agtctattct ttaaataata aatcataaga atagactttt taaatttgtg tttttataag
    39901 ataaattatt taattaaagt ttgttctttt ttctaaacga ttctttataa attcatatat
    39961 tactttcact aatatatttg tgactaatat taacacagct agtgctgctg cctgtgcagt
    40021 actcccctta tcatctagtt gtacaattga tattgcagct acttttgaac ttgcagtata
    40081 tataaaaact agtgctgata ttgtcaccat agagtttaga aaaaagtaca taaagttctc
    40141 taaaactgct ggcaaacaaa gtggtatagt tacgttctta agaattgatg cgctgtttat
    40201 tcccattgat ttagctacca tgtccaattc tttatctact ttcttaatag cagtagtagc
    40261 tgttataaat gctactgaaa agaaatgaac tatattacat ataaccatta taagtaatgt
    40321 tccatataat ccattaaaca aatttttaat gtatattgta tccataaaat taaactctaa
    40381 tttattaaaa aataatacat aagctatacc taaaactaat cctggcaatg ccataggcaa
    40441 tgtacttaaa aaatacccaa atgattttaa atatggagct ttttctgttt tttcatttaa
    40501 atatgcaaat aagaaaacaa agctcgtccc tagaatagct gtcaatagtg acatttttat
    40561 tgaatttata aatggtttaa ttccatcaat actgtttttc atagaaaagt gttccagtgt
    40621 aagtcttaaa tcatatggcc aaactgatat taatgaggat aagattacag ttaacattac
    40681 agctattata ccaaatgcaa taattcctac aacccctcct aaaatactat ctcttaactt
    40741 attagtttta accttatatg gaattgactt tgaagttata tatgagtttt gatttttttc
    40801 aattttttta gttactataa acgatattat tgcaggtatt aaaagtaata ttgatacagt
    40861 tgctcccatt ggcatattct gttgccctat aatttgctta tatacatcta ttgataatac
    40921 attaaactct cctcctatca cctttggagc tccaaaatct gtaaaacata atgtaaatga
    40981 tacgaatgca gaacttatta aagagtattt tatacttggg attgttatgc ttataaattc
    41041 tcttatcttg cttatcccca tagtattact agcttcatat agtctataat cggttgaact
    41101 tagagatata taaagtattt gaaaggattg aggaaataca tatataattt gtgccaatat
    41161 tattcccact gctccatata ttggaacatg cactccaagt aaattagtta ccaaaccttg
    41221 atttccaaat aaatatacta gacctattgc atgcatcatt gttggagcaa acataggaaa
    41281 catagctaat gatttaaaaa aacctttaaa tttaatcttt gttctagtta atgcatatgc
    41341 atatactaat gcaagtgtta gtgatattac agttgatata actgatacaa atactgtatt
    41401 ttttagagaa tttaatagag attcagaagc caagtattct tgaaagtttt gtaatcctac
    41461 aaagactcca tctttatcta aaaaagcctg tacaaataga tatattatag gtgctaataa
    41521 aaatattact aaagacataa ctaatactgt aagcatacat ttttttatat ctaaattcat
    41581 ttttgattta agacttatac ttttagaact tatagcatta ttattcatac acttatgctc
    41641 ctttaacttt tggatacttt aaatagtatt tttcgtctat acttattttt acaaggtcac
    41701 cttctactaa atttaaatta acccactctt ttatagaaac atcacttatt atgaaatttt
    41761 catttaattt atcttttatt tcaactctca ataaattccc tctaaactct atatttgata
    41821 ttataccttg gtagttttct tttgtcgatt tctctatttg aacatattct ggtcttacag
    41881 ttaatataga atctccatta tcaaaacagt ttgtatctcc tataaattga gctgtaaata
    41941 ggttttgagg attttggtat atttcttctg gagtccctat ttgcattata tctcctccat
    42001 ttagaatagc tattttatct gccatagtta tagcttcttc ttggtcatgt gttaccatta
    42061 ttgtcgttat attaagttct ttttgtaatc ttttaatgtc cattctaagc ttgtgtctaa
    42121 cctttgcatc taatgcggac attggttcat ctaaaagtaa aaactttggt tcaagagcta
    42181 atgctcttgc tattgcaatc ctctgttgtt gcccacctga taatgcttta ggatatttat
    42241 gagcttcatt agtcaatcca actgtttcaa gtacttcctt aactttatta tctatttttt
    42301 cttttgaaac ttttctctct tttagtggaa aagctatatt gttataagca gtcatattcg
    42361 gaaataatgc atatgattga aatactatac caaaacctct ctttgatggc tctaaatttg
    42421 taatatcttt atcctggaga attatagttc cactatttac atcttcaaga cctgctatta
    42481 ttctaagtaa tgtagtttta ccacatccac taggtccgag taaacataga aattcccctt
    42541 cttcaatatc caaactaata ttatttaaaa cccttttttg gtcataactt ttaaacacat
    42601 tatttatttt taagtaactc atatcttatc tctccttata tagttatttt tttattttaa
    42661 attttatatc ctactttttc ttctaaaatt ctttcatcta aattattaat ttaaacctta
    42721 cttactttca gattttgaac cgtattttga ttcccaagtt ttaagtactg tacttctgtt
    42781 tttggcagca gagtttaaat caacttcttt tataagctgt tcaaatggtt gtttagaata
    42841 accttttgga actggattat atgtctttat ggaagtaact gctacttcat gtgcatatct
    42901 atccatagct gaatcactta ttgcccaatc taaaaatagc tttgcttctt tttttatatt
    42961 atctttttta actaaggcat ttgcttctaa gtcccaacct gaaccttcct ttggaaatac
    43021 aacctctata ggttctccac tagataattg ttgtgtagct ctatatccaa gtgtaatacc
    43081 tattggatac tctccagagc cagctaactt cgcaggtttt gaacctgagt gtgtatatgt
    43141 agctatattt ttatgtaatt tatccatgta attgtaagca tctttttcac ccatcatttg
    43201 aataagccct gatactgtaa gataccccgt tccagatgat gctggatttg gcattgttat
    43261 aagtccttta tattctggtt ttattaaatc ttcataactt tttggaatat ctatacctag
    43321 tttcttaagt tccttataat taactactat tgctgtctct gggacactca ttcctaccca
    43381 agatacatca tcttcagtat ctttaaattt tttatcaact ttatcataat ttttaggctt
    43441 atatgcttca agtgcaccat ctttttttag ctctatcatg ttcattgctg ataatcccca
    43501 tataatatct gcttgtggat tatctttttc tgctaatagt tttgaagcta ttacaccatg
    43561 agaatccata actatctcta catctatatt gggatactct tctctaaaac cttccatata
    43621 ataagctatc tgctcttcct ctagtgctgc atatattgta agtttattat ttccttttcc
    43681 ttgagtagtt tgtggtgttg ttgatatgca acttgtcata aatgtaatca ctacaatgca
    43741 cagacaagta aatataaatt ctttcatatc ttcccccttt gtgttttttc ctttttttat
    43801 ttgtttttta ttgtttttgc ttatgtttat ataatacacc taatttgtta atataaagtt
    43861 aagtgtaaat taacagttcg ttaattttta gaaatttttg ttaaatatat tatttttagt
    43921 taaatacagt ctgtttttaa acaatatatt aatttttata gtatctacat tttaatttaa
    43981 acataaaaaa taagtctaac ctctataaag tagaatgtta gacttatttt tataagtatt
    44041 taattataca accaaatcat tttttttctt ttcttcaagt gcttttttaa gcaccatatc
    44101 cttaaccttc ataagtctag tagtacttgc tctctcattt tcttcaagct tcatagctat
    44161 atacttaata gtctcaatgt aatttggtat cattacattt tcaagagcat taacacgtct
    44221 acgtgtcttt tctatctctt gtgcaagaag ctgtgcactt ttttcacttt ctgcaagtct
    44281 taaaagtggt tgcatagcat ctgagaaagc ttccatagca gaatctaatt cacctgatgt
    44341 aaaagcaaga ccataaggat aaatatcact ttgattgttt tcagttttaa agtcaaacac
    44401 aggaacatca acactcatta tatttctcgt agaaacatct actgaaactg attgctttgg
    44461 catcataagt gctgaaccca gatactcctg actcataact gctcttgcga ttataaagtt
    44521 tttataagca gtatcaagag cattttctgc ttcttcacgc aatcgcttat tttctcttac
    44581 aatctcaaga aattgtttca taagttcatc aagtttatct ttaagaagct tatgacctct
    44641 tgtagcagtt ttaaggagtt tcttaagtct agtcatttcc atacgtgttg gatttatatt
    44701 taatctagcc acagatatca ctccttaggc atatattttt caaaatattc atcacggata
    44761 cgtttaagtt cactttttgg taacatacta agaagcttcc atccgatttc taatgtctgc
    44821 tcaatagttc tatcagttct aaatccttgt gaaacatatt ctttctcaaa ttcatctgca
    44881 aacttagcat acattaaatc aatctcagaa agtgcagatt caccaagtat agccataagt
    44941 tcttttgcat ccttacctct tgaataagct gcaaaaagct ggttcattgt atctgcatgg
    45001 tcttcacgag tcttaccttt accaatacct ttatctttta gacgagaaag tgatggtaaa
    45061 acatctatag gaggctgtat atctttttta taaagttcac ggctaagtat aatttgccct
    45121 tctgtaatgt atcctgtaag gtcaggaatt ggatgtgtct tatcatcctc aggcattgta
    45181 agtattggaa tcatagttat agacccttca tgccctttca tttttcctgc tctttcatac
    45241 atagtagcaa ggtcagtata aagatatcct ggatatccac gacgtcctgg aacttctttt
    45301 ttagctgctg aaacttcacg caatgcctct gcatagtttg taatatccgt tattataaca
    45361 agtacatgca tatcttgctc aaaagcaagg tattctgctg ctgtaagtgc cattctaggt
    45421 gtagatacac gctctacagc tgggtcattt gcaaggttta caaacactac agaacggtcg
    45481 attgcaccag ttgctttaaa atcgcttata aaaaattcag catcttcaaa agttatacca
    45541 actgcagcaa atacaacggc gaacttagaa tcagttccac gtacttttgc ctgtcttgca
    45601 atctgaactg caagattggc atgtggcaaa ccagaccctg agaatatagg aagtttttgc
    45661 cctctaacca atgtattaag tccatctata gcagagactc cagtttgtat aaattcggcc
    45721 ggataatcac gtgctgctgg atttattgga gctccattta tatcaagtct tttatctggt
    45781 ataatttctg gacctccatc aataggtcta cccattccac taaaaacacg gcctaatata
    45841 tctggtgaaa ctccaaagtc aagactcttt cctaaaaaac gaactttact ttcagcaagg
    45901 tttatacctg ttgagctttc gaaaagctga acaagtgcag tatctccatt tacctctaga
    45961 actttgcaac ggcgaatttc tccatttgaa agttcaattt ctccaagttc atcaaatttt
    46021 acaccttcta cgccgtttat taacataaga ggaccggcta cctcttgtat tgacttatat
    46081 tcctttatca tagttcttca gcccccctct ttttaataag gtctgcaagc tcgttgtcta
    46141 tttcatcttc aattgaatca taagctgcat ctatatcgcc ttcttctaca tacttagcac
    46201 gaccaatctt ttcaagaact gaaagcttga taatatcgtt tatagtaaca ttttgcttga
    46261 tagcatcttg tgctgactta tagaatttta atataagtcc catcattcta tattgtttat
    46321 gaagtgatgt ataagtatca acttcatgga aagagttttg atgaaggaag tcttctctta
    46381 tagaacgagt aacctctagt atcaatctat ctccatctga aagtgaatca accccaacca
    46441 attgaactat ttcttgaagt tcagattcaa cttgtaaaag tttcatagct tgagctcttt
    46501 gagctgagaa attatcattt acatttttat ctgcccatac atcaactttt tcctgataga
    46561 gtgaataact agtcaaccag ttaattgctg ggaaatgacg tttatatgct aaagatgcat
    46621 caagacccca gaatactttt actatacgaa gcgttgcttg gctgacaggt tctgatgtat
    46681 caccaccagg aggagaaact gcaccaattg ctgtaagtgt tccttctctt tcatccatac
    46741 ctagacaatt tacctttcct gctctctcat agaattgagc aagacgtgaa cctaagtatg
    46801 caggataacc ttcttcacca ggcatctcct ctaaacgacc actcatctct ctaagagcct
    46861 cagcccatct tgatgtagag tctgccataa gtgcaacact atatcccata tctctaaaat
    46921 attcagctat tgtaatacca gtgtatatag aagcttcacg tgcagcaact ggcatatctg
    46981 atgtatttgc tataagcaca gttctttgca taagtgactc gcctgttctt gggtctttta
    47041 attcaggaaa ttcaagaaga acatctgtca tttcatttcc acgctcgcca cagcctatat
    47101 atacaactat atctgcatct gcccatttag caagctgatg ctgtgtaaca gtttttccac
    47161 ttccaaaagg tcccggtata gctgctaccc caccttttgt gattgggaaa aatgtatcta
    47221 taactctttg acctgtaaca agaagtgaat ctggtgtttt cttttctcta tatggtcttt
    47281 cttttctaac tggccatttt tgcatcatag ttacaggtat atcattacct ttttcatctg
    47341 taataacaca aactgtatct tcaacagtaa attcaccaga atatatttct tttatagtac
    47401 ccttaactcc ataaggaacc ataattttac attctactac tgctgtttct tgaacgccac
    47461 caattatatc tcctgctaca accttatcac caacagattt tttaggttta aattcccact
    47521 tagttgttct atcaagtgaa gatacttcaa cacctttagt tatattagta cctgaaatat
    47581 catacatttt ttcaagtgga cgttgaatac catcataaat agttgaaata agacctggtc
    47641 caagttcaac actcataggc attccaagag aaactacttc ttcccctggt cctagaccgg
    47701 aagtttcctc ataaacctgt atagaagctt tatctccatg catttctata atctcgccta
    47761 taagatgttt atctgaaact cttacgacgt caaacatatt cgcatctctc atgccctccg
    47821 ctacaacaag cggtcctgac acctttacaa ttgtgcctgt tttcatattc tctcacctgc
    47881 aatcatatac tttatcaaaa tttaaatatt ccctcctata ttttattagc acatatcttt
    47941 agcaccaata aaatttgaga ttgtctttta ttcaactttc atctattctt taaataatat
    48001 atctgcacct atagcacgtt tactagattc tcttacttca ttcataccta atcccatact
    48061 tcctccaata cctggaataa ctataatggc aggaagttgg taatctttgt attttgctat
    48121 tgattctttt gcaagcaatg atgcctgttc tgtaatataa attatcgcat attcatcttc
    48181 aaccaactta tgtatacttt ttttaatttc gtcactatca taggctggga agatatctat
    48241 tccaagagct aaaaatccca tgatgctatc tttatctcca actactccta ccttatacat
    48301 aagcatccct tagcctttct tttatagtat ctgcgtctat attattaagc ttactagtca
    48361 aaataattct aattgttttt atctctgatt ctttagcata taaatatgca attagtggtt
    48421 ctaatgtaag aggcttaaat tttgctcctc taacatattc cataagatag tcatcacaaa
    48481 gtttttcaaa gacagtaaaa ccactatcca tgccattttt acagacatca gagtaagaag
    48541 tagatttaaa acatgatgct ggagtatctg aggaaaaagc ttctaagaat ttttccttat
    48601 caagtgagcc acctgaaaca aacacattca taaacatatc aaaatctttc ttcatatttt
    48661 tgactcttat aaaactcttt agatttgtta aatcgcaaac tttcataaca taatctatta
    48721 caaatttgtt ttggctttct ttagcagttt ctttcatact ggtaaaggtt gctttatcta
    48781 gaacaatatc aaccatctgg ccattttgtg tcttattata aacatcaaat gcctctaata
    48841 tagctgatga cattatctta ggtaaatcac taaaactacg atttgctaaa gattctctaa
    48901 gcttaataag aggaattgta cctccatcaa ttaaatattt ctcaccatct acaccagaaa
    48961 tttcttcttt aatcagcact tttaaattgt gatagtcatt tttataaaga aatacatcaa
    49021 caaatttttc ttcaggagct agacttttaa gaatttcata tgtctttgat aattcttgag
    49081 tcaaaatatt ctcaaaatta tgtatattat tatttgatgt atcagcataa cctgcctcag
    49141 ctataattct taaagagtct tcagggcttt tggcttctgc catttgaata aacatttgtc
    49201 tgttaagaag ctgtttttca agcactctta ttcgtgcaac ggcataggga taagtttttt
    49261 cttctgccat tttttaaccc ctccttaatt aaacaaaatt tctgctgcaa tttgttcaat
    49321 atattcatgc tctactgcaa ttatggcttc aaatgaataa ttgtactcaa tgtcaccttt
    49381 tttaactatg aaacctccag taatatctct agtttcatca gacactttaa tgcctttttt
    49441 tgatagcaca tcttttaatg tttttttatc ttttttagat aatactatct ctgaatcatc
    49501 tgtacaattt gatttttcaa tcatgtttag tataatctct tcatattcat tatctttaag
    49561 atttaaaagt ttgtttttag cttctaaaat tacctcttct aaaatctctt gtttttttga
    49621 taatatttgc tttttagcct ccatataagc tcctgatatt tccttagaag ccgctttttc
    49681 tgcttctgcc tctgcaagct ttgtatagga agccatttcc ttttcagctt tttcattagc
    49741 tgaatttact tttaaatctg cttcactttt agctttatcc aatatttctt gtgcttcttg
    49801 tttggcatca gctataattc tgtcaatcat tttttgttca ttacccatag gagtgcttcc
    49861 tccttttcat tttttttact tatgaattat aatttataat taaccaattg caattccatt
    49921 gtaagctaag aaagaaacaa gtagtcctaa aagagcatat gtttcaacta ttgctgcaaa
    49981 tgttatagct ttaccaagtt cttcaggtct ttttgcaact attccaactc ctgctgctgc
    50041 tgctttacct tgtgctatac ctgatactaa accaactaaa ccaattggaa gtcctgccat
    50101 aagaagttgt aaaccttgag ctgatgttgg tattgcatct ccaccaataa tccctgcttt
    50161 tgataaaata aggaaagcta tgataaatcc atacagacct tgtgtaccag gaagaagttg
    50221 taaaactaga agttgaccga atttactagg gtcttctgtt acaacacctg ctgcggcttg
    50281 acctgctatt gaagtacctt tagcagaacc catacctgca aataatgctg ctatagcagc
    50341 tcctgatatt gccaaaaatt gaccatttcc taacgcgttt aaaaattcca tatttacatc
    50401 tcctttttat taatgcttgt atattttgtt ttatacttaa acggaacaaa aggaactcct
    50461 ccgccttcat aaaacttatt gaaaaattct acatactgta acctacttgt atgaacatat
    50521 gcacctagtg catttataag aaggtttatt gtgtgtccta caacaccgat tacaactgca
    50581 agtatcggac cagctatagc ccctaaaagg ttgataactt gtgctataac tcctgttgtt
    50641 aaacaaagcg ccataattct tgtataagat aatatatcag caaaataact tgttatgcca
    50701 tagagacttg aaaatccttt aaaaagctta acaggaatac ctttaaagct tcttccttga
    50761 gtaagtacaa gacctatagc acctataatt gctaaatatt ttcctacttc tgaaaataca
    50821 cctatgtccc ctgcaacaaa aggtattata agtagacata cacctgttat acacaaatac
    50881 caaaaaccta tgtcaaagat tgcatctatt acctttccac ttcttattaa tgtatatgct
    50941 tttattccca aacctacata tatatggata ataccgaaca gtaacgacat tcccataagt
    51001 gtcataacat cctctaaagg attaattaat gcttttatag gaattaaatc ccccaaaata
    51061 cccccgaata taagacccca tacagtagtt gaaactccac agataccaat aagtttaatt
    51121 aaatttcctt cacctttttg tatctttaat ttatatacaa caaatccaca gactactgca
    51181 attattaacc catatgcggc gtcacttagc atcattccaa agaatacaac aaagaagaaa
    51241 gttaaaattg tatttgggtc aatatctttt gtacttggat agctgtacat gttagttaca
    51301 ctttcaaaag gtgttactaa tttattgttt tcaagcagaa ttgggaagcc ttcttcttta
    51361 cttccttctt cagtctgaac acaacaatca aacttttctg taatttcttt aattaattca
    51421 tctgcccttt tgcttggtaa ccaaccatta agacagaatg ttgtttttgt tttgacaagt
    51481 ctctctataa tctttttttg gtctctttct atagtgtaaa aatcatatag gttttcaagc
    51541 atgtgtatac tatctgcatg tcttttaatt tcctctgttt tgtccttaga aacgttttcc
    51601 tgcctaatta tcatttcatt gtatttcttg atagcttgta acggtgtacc ttcttcattt
    51661 ggtaatgaaa ccactgaaaa attaaattct tttaaaactt ctaaaacatc atcaaaagtt
    51721 tcttttaaag taattaaata aacatagttc attaacttgt cagaattgac aatcccaata
    51781 acactttctg gtatttcatc ttctaatttt actcttaaag catcaatatt tgcgcttatg
    51841 ggcacagttc caagtatagt ttttgtatac ttagtttcca tattttcaag aggaatatca
    51901 aagttcaccc atggttctaa catactttta ttgttgttaa gtaagtttat gttagacctt
    51961 attgtaccta tctccttgtg caaattgttt aactccagag cttcagcata aacttgccct
    52021 tctttttcac tatcaatttt gttaaactct ctttttggtg caagtaggga ttttttttgt
    52081 ttcactagtg aagaaacaat attaattgct tggtttacat gagacatttt ctgctctagc
    52141 attataactt cagattcttc actatctttt acaagataat tttttaattc ctcctcctca
    52201 gtaagaaagg cactatcatc aatctgaaca aacccacggt tcataagtag ctttaaaatc
    52261 tggctttttt gagcttctaa gccaacaata gatattttgt tcatattaac tattgccata
    52321 ttatctcact acctttccaa taaccgcttc aatagcctta ctttttcttt cttcagcagt
    52381 tttttcaatt ttcagacatt cacttttagc attagctaag attttatcaa cctctgctct
    52441 actgtcggtg actgccttct caataagagt ttcttccata agctttgctt gttttcttac
    52501 atcagcaaat atgctttcac atttgtttac agcatcagct ctgatagact ctgcatcagt
    52561 aacagctttt actttgatac tgtctgctgt ctgctctgcc tcaacaatgc tttttattat
    52621 ttcaaaagcc atccaaatca cctctcttac ctttttacta tgtatataac caattagatt
    52681 tttttattct aattagatta ctacctcatt gttaaagtat aaacagcatt tttatataat
    52741 aattaatatt tttcaacgtt tatttcttaa taaaacatat tttagaatag tacttgccta
    52801 gatttacatc cttttttgac ttacttttag actatgacat gagtatggtt tttaaatacc
    52861 attattttca ataattataa tttaaagcga tatagtttgt caatagcgtt attttgtttt
    52921 aaaattaacg ctatgtcgac attttaaatt tttattttat tttttttata attttaattt
    52981 aatttattac atataaaagt tttttatatt ctttttcaac ctctaaccct tgaagtttat
    53041 tgtttttttt gaatttttag aaatatctcg cactatttta tctaactttt ataaatcatc
    53101 aagctatatc gatttttgtc accttatttt caatctaaat tttttatata tagttagaaa
    53161 taaatcaata tttttaaaga ttgataaaac ttacacttag ttctaatagt tttattttaa
    53221 ggcaatgtat taatcttaag tagataatat cattagttac tctatatatt cgacaacatt
    53281 ataaaaaaat cctcgtacca atcactccaa tcttcaatag gatatgctgc tcctccattt
    53341 acacttttca gtaggtttga tgtatattct tctcccatgg aaaaaagttc ccctactgtt
    53401 atatcataac ctctagatgt aacaacctta caaaatgaat ccataacatc acgttgttct
    53461 cttgttttta taagttcctt tctaaattca tcatcattct tagcttcttc taaaatttta
    53521 gttaattttt cattcattat gtatttctcc ttttaagtag tctttctgta aacttcaata
    53581 agctttatat aaaaaggaag ccatcctaaa taaactaaga tgacttcgct taactgttag
    53641 caacacattt actagtataa aataattggt gtacctggtt ctatattttc atatattttc
    53701 tttgccttag aaaaaggtgt atttacacat ccatgtgaac ctgaagactt atatatacct
    53761 ccaccaaaag aatttctcca agtagcatca tgaatgccta tgttcccatt aaatggaagc
    53821 caataactta caggtgaact gtaaccttga ccagttaatg aaacatttct agccttataa
    53881 ttaagaggat atatacctga tggtgtagca aatcctctag ctaaatcgcc tgttacaaca
    53941 tctgttgata caagcatttc tccattttta aagaaccaca tatgttggcc agaaagacta
    54001 atctctacat atgtatttcc aatatcatct ttattacgag atacagcttt ttgtatgtaa
    54061 ataggttctc ttgtaacatt tttaccagct tttacaacat caactaatgc ttcagcttct
    54121 ttctttttat ctatcaacca accataaatt ccacctgaaa ttgtaatatc tttacctgtg
    54181 gcagttttaa atggtcttga gtcaccaaaa gtactgtatt ttttacttaa acttcttgta
    54241 tattctacca tagcatcatt atcaaaagtt atgtcataat tcttttctga gtctactttt
    54301 aaccatttat taatttcaga accatctaaa gtttccttac ggtcactaaa atcatatgta
    54361 acgactacac caacatactt atctaatgtc ttaatagctt gtttcaactt ttctgaatcc
    54421 tttgtgttgt ttggtgcttc ataaacttta ctttcatcca gagaaataga agtattccca
    54481 gaattaattg agttttgtac taacttataa aaatcatctt ttttcacttt gtttccaagt
    54541 acctctggta ctataacata agcgttttta gtgctatcat acttaggata agcactctta
    54601 ggttctacaa catttttact atcaaaacaa gataaagcat taaacctttt ttctagtaaa
    54661 tctttattat atgaaagagt tatcatatta ttatactctt tagatttaaa tattccacca
    54721 atccatccaa tagcgctttg actatctttt atcttttgaa ctttatcatc tggtgtaaat
    54781 tttaaatcaa tatcttttgc ttttatttgt tctttcttat tgtttctttc ctctaagttt
    54841 aaagtatagg aattagtact agatgttaac aaatcattta cttgctcaac tgtttttcct
    54901 gaagccttta cgccattaat agtcgttcca aagtaaaaat gatttctaaa aaagattgcc
    54961 attcctatat aagctacaaa caaaacacct acaactatag ctatcttaaa ttttagattt
    55021 ttttgtccac tttcttgttt tacctctttt ccatcaatca taattaaatc cttccttaca
    55081 ttgtaattac ctatgtactt tattggtttt taaacaaaac catgtatgcc ttattttata
    55141 tacccaaatt actgtatcat tgtttatatt tttaaatata tagttatttc cataaaacca
    55201 ttttatcttt tagacaaaat gactattata aatcacccct ttcaaaatcc cttaaataca
    55261 atctctgcta tattaatata ttaccatact tttaataatc gtgggttatc tatctgtttt
    55321 tgtaacacta aactactctt ttaaaaaaga aactaatttt atgttagttt ctttatctta
    55381 acttattttt atattgaatt ttagcttaac ataacgttgt tatatagatt ctatttaatt
    55441 attttagttt ctatatcttt tccatcagca tattctttaa gtaactcctt agcatatcca
    55501 aaatattctt caatatcaga ttcttgacta aaagaaaata tacaaattaa tatttcattt
    55561 gcatcttctg ttatcattgc tctttcagaa tcactagttg gactcccaaa tttacccact
    55621 gaatcagaca atataggaag attttctata tttattaatt ctttcccaat acccttatac
    55681 tgttctcctt catctcctac agtaaaacat actggtgaat gtaatttgct tacattatat
    55741 gtacctactg gatataatga ttttattgat attaaattgt ttatttctac tatattattt
    55801 attgtataaa gacccttccc ttgaataatt cttctaacca atgcttctga agatgttcta
    55861 tatctacttg gattttttcc aagctcttta taagcatttc ttgcatcttt aattttatct
    55921 agtttcgtaa tgtcttcaac ttttagactt tgatttatag tatcacaaaa ctcatttatt
    55981 tcattaatta atttttctga gcttttattt actgctactt ttgcttgaat gcaacccaac
    56041 ttagcattag gccatctttc cttaagtttg tcatcaatac tcacatctat catacttaaa
    56101 caactcctat acactaaatt tttattaaag tttttatcat ctatacatta tagtactatt
    56161 cttttgttaa taacatgttt attagcaaaa taaatttatt tttaatataa tttttataaa
    56221 atacaattat tttttgcttt atattaaaca atacattgat aaaataaaaa aacactaatt
    56281 atttagcagt gatttattaa aatcaaacat tcataatagt gctttatata aaaattaaat
    56341 tttgttttaa agtaaatcta aaaaattctt agctggcatt ggtcgtgcaa aataaaatcc
    56401 ttgaactaaa tcacatccaa attgtttcat taactcagct tgttctttac tctcaacacc
    56461 ttctataatt acaactaaat ctagtgattt tgctagagac actattgatt ttgctatatt
    56521 gcgacttctc tcatcatgtt caataccatt aagaaaagac ttatctagtt tcaatatatc
    56581 aattggcata tctcttaaac aactaataga agaatatcct gtaccaaagt catccataga
    56641 aatagaaaat ccatacttcc tcaactcatt taaaacattc tttaaaatat ttaactcatt
    56701 aatagccaca gtttctgtta tctctatttc aattaaatct gaacttagat tatacatttg
    56761 tattgtttta ttgataaatt ttacaatatc tggttgatac aactcaactc tagataaatt
    56821 tattgaaact ggaactatct ttttaccttg ctttttccat tcagataaat cattgcaaac
    56881 cctttcaaat acaaatctcc caatttttaa aatcaactta cttttctctg ctattggaat
    56941 aaatactgcg ggacttataa aaccatgttc tggatgattc catcttatta gtgcttcact
    57001 ccctatcatt tctccagtta caagtgaaaa cttaggttga taatacactt caaattgatt
    57061 ttttaccaat gctattttta tatcatctaa aatcattgat tcttcactaa gcttattcct
    57121 aacatcttca ttataaatag cataatttat atttttacct ttagcaacag acctagccat
    57181 atttgcttta tttacagcct tttggatatc tacttcacct tcttcaacaa aataaatacc
    57241 cacacacaga gaaatttcaa atactatact caaattactt tcaatattat ttcgtatact
    57301 atcaattttt cttatacgtt tattttttcc atttttataa tcacagaaaa ttgcaaaata
    57361 atcacttgaa agtcgtgcat atacttcacc ttctgttaat tcttctttta aagcttttgc
    57421 tatcgtaatt aataaattgt caccaaattc atatccaaaa gtatcattaa tcatcttaaa
    57481 gttattaata tcaaaatata ctataatata atttaaagat ggtttatcta aaagatactt
    57541 actacactct ctataaaatt tattttgatt tcctatatgg gtaactttat cttcataagc
    57601 caaacttaat atttcttttt cattggattt ttttatataa attatataaa aaataattgc
    57661 aataaatatt aatataataa ataacactgc atatatagct ctgttgatta tttgttctga
    57721 attactaaat acaacagttt ctggaattga aaaaatcaaa ttccaatctt ttatataatt
    57781 attttcacta ttcccattat ccatagtagc atatcctaaa tatcttcttt cttccaacat
    57841 atttaataca gttgcacctg ttttattttc ttgcaattct ttttttaatc tatttatttc
    57901 tcgaatatca ttatcctgtt ttaacaattt ataaatattt tttgataggc tatcctcttg
    57961 cggatgaagg ataattgtac catcatttct tacaacataa gatatactat tattttcata
    58021 aaaagataca ttaataagtt tcatcagagt attgatattg taagtacaat ataaaactcc
    58081 aactacttta tcattattta ataatggtac tgaaatagta atcttcttta tagacttatt
    58141 tacttcatca acatatggac ttgaaacata cttcttacct ttaattgagt ttttgaaaaa
    58201 ttctctatct ttcactgaaa acttatcacc tttggtattt tcagcattac catttatatc
    58261 aactattgcc atagtgtaaa atttttgatt ttccatttca tctaaaagat ttttcatttt
    58321 ttcctcatta ggtatatctt gatttaaagc ataatagtta gaaaaaatat ttaaaacatc
    58381 taaatctttt tcaatttgtc tttgtgctag ttgagagcct tgtactgcaa cttcttttat
    58441 atgtattttt gcctctgaca taagaagatg ttttgtatct tctatataga tataccccat
    58501 tgtcaaaaat aatgatgtta aaaaaagaat tgtaattaat gtaaaacttt tatttctccc
    58561 tgtcattcga ctcctcccct aattagtcaa tacttcatat agataatata tcctttttat
    58621 accgattcat catttatatt gtatataact attcttaaat gttacacata aaatcaacct
    58681 aataaccata aaaacattct tttattatca taataacact atttagttga attttaaatt
    58741 aaagaataaa aactatcaat ttttctaaac aaaaaaactc ttatacaata aaactttgtt
    58801 ttatgtataa gagtttttat tttatctatt ttatagtaaa tttatcttcc ataatatgaa
    58861 tttaaataca tatcttttat ttcattcata agtggatatc ttggatttgc tcctgtacat
    58921 tggtcatcaa atgcttgaat caccatttca tctattgaat ccataaactt aggttcttca
    58981 actccaaact cttttataga tttaggaata ccaactttaa gttttaaatc ttcaatagct
    59041 accaataaac tttggaattt ctcttcatca gaatttccct ttaatcctaa aaatgaagct
    59101 atttcagcat atctttttaa tgcttctgga tatttatatt gagagaatgc tcccattttc
    59161 tttggtgcat ctgaagaatt aaatttcata acttctgtta tcaaaagagc atttgcaaca
    59221 ccatgtggta catggtggaa agcacctaat ttatgagcca tagagtgaca aacacccaaa
    59281 aatgcatttg caaaagccat acctgccatt gtagaagcat ttgccatttt ttctctagct
    59341 tctgggtctt ttgcaccatt atcatatgca cgtggaagat attcaaatat actcttaatg
    59401 gcttgtaaag ctaatccatt tgtataatca gttgcaagca tagatacata tgcttctaat
    59461 gcatgagtta atgcatctat accagaagca gatgttaagc tttttggcat attcatcatc
    59521 atatcagaat caactatagc catatttggc atcagttcat aatctgctaa tggatatttt
    59581 acccctgtat cttggtcagt tataacagca aatggtgtta cttctgaacc agtacctgct
    59641 gatgttggta tagctacaaa attagctttt tcacccattt tagggaatgt atataccctt
    59701 tttcttatat ccataaatct catagctagg tcttggaaat ctacatctgg atgctcatac
    59761 aatgtccaca ttatctttcc tgcatccatt gcacttcctc cacctataga tattattaca
    59821 tctggattaa attctctcat agctttagcg cctatcttag cacattccaa agttgggtct
    59881 ggttctactt caaagaatgt agtgtgctta atattcattt catctaataa atcagtaact
    59941 gctttagtat atccattatt gtaaaggaat gtatctgtta caataaaagc tctcttctta
    60001 ttcattacat ctttaagttc ttttaatgct acccctaaac aacctttttt gaaataaact
    60061 tttcctggtg atctaaacca aagcatattt tctctcctct cagctactgt tttaatatta
    60121 agtaaatgct taactcctac attttcagat actgaatttc caccccaaga cccacatcct
    60181 aaagtaagag aaggagctag tttaaaatta tataagtccc ctataccacc ttgagaagaa
    60241 ggagtattaa ttaaaattct acaagttttc atggctgaag taaacttatc aagtttttct
    60301 ctttgattta catcatcaat atataatgaa gatgtatgcc caaatccacc atcttctact
    60361 aatttttctg cttttctaac tgcattatca aaatcactgg ccttgtacat tgcaagtact
    60421 ggtgaaagtt tctcatgagc aaaagcttct tctatttcaa ctgattctac ttcacctatt
    60481 aaaaccttag tatctactgg gacttcaaag cctgcaagtt ttgctatagt acaagctgtt
    60541 tgcccaacta ttttagaatt taatcctcca tttactaaaa ttatatttct tattttttct
    60601 gtttcatctt tatctaataa ataagcacca cgctctttaa attctttttt gacttcattg
    60661 tatatatttt ctaatactat aaccgattgt tcagaagcac atatcatacc attgtcaaaa
    60721 gtcttagata caagaattga gtttacagca gtttttatat ctgcactctc atcaattatt
    60781 gctggagtat tacctgcacc aactccaata gctggttttc cagatgaata tgctgattta
    60841 accattccag gacctcctgt agcaagggtc aaatccactt ctttcataac agttgtagtt
    60901 aattctaaag atggctcatc tatccatcct attatgcctt caggagctcc agctaaaaca
    60961 gcagcctcaa gaacaacttt tgcagcctct atagtagaat tttttgctct tggatgcgga
    61021 gagaaaatta ttccatttct agtttttaaa gctaaaagtg ctttaaagat agcagtagaa
    61081 gttggattag ttgtaggtac aactgctgca ataacaccta ttggctcagc aattttagtc
    61141 ataccaaatg cttcgtcttt ctctataaca ccacaagttt tagtttcttt atatgcatta
    61201 tatatatact ctgaggcata atgattcttt ataaccttat cttcagcaat tcccatacca
    61261 gtttcctttt gtgccattat agctagagga actctttgct tattagctgc caaagatgct
    61321 gctaaaaaaa ttttatctac ctgctcttga gtatatgtag caaatatttt ttgagcttct
    61381 ctgatttttg ctagtttttt aactaagctt tcaacgttat ttacagtatc taaaccaact
    61441 gacataactt cattttcttt tttattttct attattactt gttctttatg agccatttta
    61501 acaacctcct gagatagttt tttatttatt atgttaattt attaacatta tagcatttac
    61561 tttttctatt ttcaagttat ttttttaaca ttttattttt tatctaatat aacatcattt
    61621 ctactaataa t
//
LOCUS       DAGQWR010000003        59254 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.003, whole genome shotgun sequence.
ACCESSION   DAGQWR010000003 DAGQWR010000000
VERSION     DAGQWR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59254
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.003"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(97..2160)
                     /locus_tag="MIW95_000121"
     CDS             complement(97..2160)
                     /locus_tag="MIW95_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBY3149481.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFND
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDVYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(2541..5876)
                     /locus_tag="MIW95_000122"
     CDS             complement(2541..5876)
                     /locus_tag="MIW95_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBY3149482.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEKLRKSLTEKRKVNTVNYDFKIDEVNEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGKYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMVTAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(5926..6354)
                     /locus_tag="MIW95_000123"
     CDS             complement(5926..6354)
                     /locus_tag="MIW95_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149483.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(6344..6724)
                     /locus_tag="MIW95_000124"
     CDS             complement(6344..6724)
                     /locus_tag="MIW95_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBY3149484.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(6757..7065)
                     /locus_tag="MIW95_000125"
     CDS             complement(6757..7065)
                     /locus_tag="MIW95_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149485.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(7098..8282)
                     /locus_tag="MIW95_000126"
     CDS             complement(7098..8282)
                     /locus_tag="MIW95_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBY3149486.1"
                     /translation="MVDEAKILDVVSISRENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            complement(8286..9749)
                     /locus_tag="MIW95_000127"
     CDS             complement(8286..9749)
                     /locus_tag="MIW95_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBY3149487.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVKVLEKEIVDGLEELERMLEV"
     gene            complement(10259..11128)
                     /locus_tag="MIW95_000128"
     CDS             complement(10259..11128)
                     /locus_tag="MIW95_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBY3149488.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIGTVKC"
     gene            complement(11148..13064)
                     /locus_tag="MIW95_000129"
     CDS             complement(11148..13064)
                     /locus_tag="MIW95_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBY3149489.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDGHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMISATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(13067..13987)
                     /locus_tag="MIW95_000130"
     CDS             complement(13067..13987)
                     /locus_tag="MIW95_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBY3149490.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(14089..14841)
                     /locus_tag="MIW95_000131"
     CDS             complement(14089..14841)
                     /locus_tag="MIW95_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY3149491.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(15138..15755)
                     /locus_tag="MIW95_000132"
     CDS             complement(15138..15755)
                     /locus_tag="MIW95_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149492.1"
                     /translation="MNVIKKFIFVILIINILGCGISLILQSSLGSDAITLLNEGIHIK
                     LGISYTIAGLIYNGTLLLIALLLNKKSLGLGSCVYVLVAGIFIDLYIYLLLDFNISSL
                     GIIIRIMSFIIGHILMCSGFAMLIKFDIGMSPLDAILIYIEGKFKYPYKVLKTIADLI
                     FLILGVYLGGNLGFGTIFSILCTGTTISIVSKMISVNKKRYYSSI"
     gene            complement(15843..17300)
                     /locus_tag="MIW95_000133"
     CDS             complement(15843..17300)
                     /locus_tag="MIW95_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBY3149493.1"
                     /translation="MIKLSKFPKDFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKHYESDMTVDKIKFAMKDNNGYYPKRNGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKDKVKYWIVINQINLMYHESFNSVAFCVDQVDDVEEAKYQ
                     AIHHQFMATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRIQYFFT
                     DVSFRGEYPAYIKRFFKENNINIVMEEGDDKVLKENTMDFLALSYYYSTTVSASKNTM
                     NPIDNVKNQYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYKNVIRTNGEELKEKVDSF"
     gene            complement(17322..17675)
                     /locus_tag="MIW95_000134"
     CDS             complement(17322..17675)
                     /locus_tag="MIW95_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY3149494.1"
                     /translation="MTTEEHVIDEELVEVAMQIILRAGEARTEIKHALNDLERFDYKN
                     ADLKLAKAKEFMTEAHRAQTNIIQGEASGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(17704..18951)
                     /locus_tag="MIW95_000135"
     CDS             complement(17704..18951)
                     /locus_tag="MIW95_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY3149495.1"
                     /translation="MGRLFMNKFIDLLSEKFTPVVNKITKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVALVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDSMLPIAVIMFIGWGLIYGLNFDMFEIIVRIFKPVSDIAQTLPGFV
                     LLNFLVVFLYSLGISGWVLSPITYAIQLSAIAANAAAVASGGLATNIYTYEVVQTGWI
                     TLGGWGATLPLVLMMCLSRAGRIKAIGRACIVPSIMNINEPVMFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYLPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEELKLESQEA"
     gene            complement(18959..19276)
                     /locus_tag="MIW95_000136"
     CDS             complement(18959..19276)
                     /locus_tag="MIW95_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3149496.1"
                     /translation="MKNIKVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLPEIDCLLLGPHLDYMKDEIQKKADEYNVPVACISKTAYGRLDGVKALEEALELI
                     LNK"
     gene            19524..21437
                     /locus_tag="MIW95_000137"
     CDS             19524..21437
                     /locus_tag="MIW95_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY3149497.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     STGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNLTLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYKNKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(21560..22714)
                     /locus_tag="MIW95_000138"
     CDS             complement(21560..22714)
                     /locus_tag="MIW95_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBY3149498.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(22996..25611)
                     /gene="mngB"
                     /locus_tag="MIW95_000139"
     CDS             complement(22996..25611)
                     /gene="mngB"
                     /locus_tag="MIW95_000139"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBY3149499.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNKDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YNRNEIVNAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKISDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIINVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGKKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKIPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANVVFNKNIKTAKWTNLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(25755..27680)
                     /gene="mngA"
                     /locus_tag="MIW95_000140"
     CDS             complement(25755..27680)
                     /gene="mngA"
                     /locus_tag="MIW95_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBY3149500.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDSNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIVLAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKKADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVIGKPVAA
                     LNNGLINWLNGMTGINAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIHVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(27810..28535)
                     /locus_tag="MIW95_000141"
     CDS             complement(27810..28535)
                     /locus_tag="MIW95_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY3149501.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(28798..29577)
                     /locus_tag="MIW95_000142"
     CDS             complement(28798..29577)
                     /locus_tag="MIW95_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBY3149502.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(29570..30997)
                     /locus_tag="MIW95_000143"
     CDS             complement(29570..30997)
                     /locus_tag="MIW95_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY3149503.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAVSAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGHEVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            31388..32110
                     /locus_tag="MIW95_000144"
     CDS             31388..32110
                     /locus_tag="MIW95_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY3149504.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECIGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            32307..33974
                     /locus_tag="MIW95_000145"
     CDS             32307..33974
                     /locus_tag="MIW95_000145"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBY3149505.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNLENEKVREECYKVLEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(34070..35389)
                     /locus_tag="MIW95_000146"
     CDS             complement(34070..35389)
                     /locus_tag="MIW95_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY3149506.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYADKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(35430..36176)
                     /locus_tag="MIW95_000147"
     CDS             complement(35430..36176)
                     /locus_tag="MIW95_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBY3149507.1"
                     /translation="MKKLVKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(36420..38429)
                     /locus_tag="MIW95_000148"
     CDS             complement(36420..38429)
                     /locus_tag="MIW95_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3149508.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEEIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQSFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQGVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYSITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(38867..40300)
                     /locus_tag="MIW95_000149"
     CDS             complement(38867..40300)
                     /locus_tag="MIW95_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBY3149509.1"
                     /translation="MVLRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSREVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMTKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(40387..42303)
                     /locus_tag="MIW95_000150"
     CDS             complement(40387..42303)
                     /locus_tag="MIW95_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY3149510.1"
                     /translation="MRRYEQLSKEIIENVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASIGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVIIIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGVLTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTSVVAAMYFKLKDKKIKELCIPAVISGICGVIEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDSIVEISKDNNGQGVVEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTIMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKHFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIIENTQ
                     KHVNNSQELITVLA"
     gene            complement(42377..43237)
                     /locus_tag="MIW95_000151"
     CDS             complement(42377..43237)
                     /locus_tag="MIW95_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY3149511.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKCIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(43588..44775)
                     /locus_tag="MIW95_000152"
     CDS             complement(43588..44775)
                     /locus_tag="MIW95_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY3149512.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGDNKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIESGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(44789..46180)
                     /locus_tag="MIW95_000153"
     CDS             complement(44789..46180)
                     /locus_tag="MIW95_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBY3149513.1"
                     /translation="MSSLKKKKFTMPQTLTIIFSLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMVFLTIGLYLGF"
     gene            complement(46775..48475)
                     /locus_tag="MIW95_000154"
     CDS             complement(46775..48475)
                     /locus_tag="MIW95_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY3149514.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLASNGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(48447..49850)
                     /locus_tag="MIW95_000155"
     CDS             complement(48447..49850)
                     /locus_tag="MIW95_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY3149515.1"
                     /translation="MIVNKELIIDYLKSNQENIIKDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGENGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTDDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSSAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(50114..50605)
                     /locus_tag="MIW95_000156"
     CDS             complement(50114..50605)
                     /locus_tag="MIW95_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY3149516.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPNEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(50631..51443)
                     /locus_tag="MIW95_000157"
     CDS             complement(50631..51443)
                     /locus_tag="MIW95_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBY3149517.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLMGYEYKKVLSKEAIKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(51456..52607)
                     /locus_tag="MIW95_000158"
     CDS             complement(51456..52607)
                     /locus_tag="MIW95_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBY3149518.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            52877..53518
                     /locus_tag="MIW95_000159"
     CDS             52877..53518
                     /locus_tag="MIW95_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBY3149519.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CTNLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTVDGANTFERDWSKVNK"
     gene            53544..55088
                     /locus_tag="MIW95_000160"
     CDS             53544..55088
                     /locus_tag="MIW95_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY3149520.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AVFELATVNKSYTELKEILFSNSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(55305..55931)
                     /locus_tag="MIW95_000161"
     CDS             complement(55305..55931)
                     /locus_tag="MIW95_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY3149521.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(56066..56782)
                     /locus_tag="MIW95_000162"
     CDS             complement(56066..56782)
                     /locus_tag="MIW95_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBY3149522.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            57197..58411
                     /locus_tag="MIW95_000163"
     CDS             57197..58411
                     /locus_tag="MIW95_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBY3149523.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            59030..>59254
                     /locus_tag="MIW95_000164"
     CDS             59030..>59254
                     /locus_tag="MIW95_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149524.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKP"
BASE COUNT        19320 a        10564 c         6402 g        22968 t
ORIGIN      
        1 cttgatataa atttgagcta taaatctata tcaagaggtg ctattttatt tttaatataa
       61 ttaatattct attacgcaaa aaatgcatat atttcattat tttatggaaa atattacttc
      121 atttaactct atatcaaact cattaagtaa aaatctaatt atatttaatt tagcctctgt
      181 atttaaattt gtttctaaat atatattttc tgatatttta ataccttttc ttaatttata
      241 tacatctcta gacttaatat tatctataaa atctctttta taagtcttgt ctacaatact
      301 gttaaacctt acagaatcca aatcatataa agtaagagca acttgttcat aaaattctgt
      361 ccaattttta gccttatatg actctcctaa tatactaaat gatactatac tttcatttgt
      421 aaaattaaca tcatcatcct ctaacgtaaa tatattttca ctgttacttg gaggttcata
      481 tgtaacactt ggatatttcc aaatttctac agctctatct agtaatatct tagttctatt
      541 ttttatttct tcttcattcc atttatctat agatgaaata tatttattta gataaagcct
      601 actatcttta aatccatctt ccatttcttt cttttcttca aatgttttat tacttagttt
      661 tgaattatat cctgtcaaag ttaaatttcc aattgtatgt aaatatttat catgtatctc
      721 cttatagtta tctcctagtg attccttcca tttatttgtt aatgtctgtg gcattatatg
      781 ctctatattt agcatattat tattgattaa attttctaaa tcaacaattt ctttattatt
      841 ataattctct aacctctcaa gtatatataa cttattcttt gacttagtat tatatatatc
      901 tttagtttca aaagcatttg aaaattcact gtcagtagga aatctttgag atgatttctt
      961 atttataaaa atatatctta atatattaga atagttttct ttataatcac taaatttttt
     1021 aatttccctt cctaagttca taaatacttt attcagtgca ttcggaggta catcacatac
     1081 tattcttctt aatataaaac attctactaa attaagggat tctacaagta cttttttatt
     1141 tattatacct tcattataat cattaaataa ttctaatata aacggatatg atacaaatat
     1201 ttcaagtttg tttatccttt tcaaaacttt gtttacatca ttatcatcag tacatgaata
     1261 agatatctct ctataatagt tagaaaattt aagtaaatct tttaataaaa tttctatatc
     1321 tataccatta tctgatacat accttttaaa actaaaatat actttatttt tattcggtat
     1381 acttctctcc tttatagtca aatagtctct tataaagcta cttacgttgc tacttgtatt
     1441 tttttcaatt ttataccaat aattattata aaattcctct tgtaacttag agcttctacc
     1501 cattaacaca aagtttctta ctctatcagc ttcacttaaa tctaagccag ttgaatttag
     1561 actttcaaag attaattgag ggtcatcttc accatttttt aactctattt ctactatagt
     1621 taatttctgt atagcatcaa acaaatcatc tattgatata ttatcttctt ttattctatt
     1681 ataaaaatat aaataatttg aaactatatt agaatttcta tctgtattca tttcttcttc
     1741 aaatagttca tagtaagctt ttctatcttc tgctattggt tttaacttta tttttccatc
     1801 tttatctctt gagtgcttgt ttataagata acactcctta atctgttctt ctgttgtaat
     1861 aattcctttt gattctccaa actcttttat tatttgacac atggcaatca ataatattga
     1921 cacagtagtc actctttgtt gtccatctat tattaaatat tcttttccca tactatatat
     1981 agatacaact gaccctaaga aatgagttct aaatccagaa tctattatat cctctaaatc
     2041 atcaaacaat cttttacatt gttctttctt ccagttataa tttctttgat atacaggcac
     2101 tacaaaactt ttgtttgacc cctctaaata actaagaaat tcaatttctc ctgctttcat
     2161 attaactccc cctataagtt aaataaattc cattcataac tccatgtatc tatatattat
     2221 atcaaaattt tgactcataa atgtatatga aagccttatt atataccttt ttatacctat
     2281 ttttatttta ttaaagtaaa tacatgatta ttctataaaa tatacaaata cttaatttta
     2341 aaaattatta tttaatcttc ttcatgagta attcatagtt atttatcaat taaaaagaga
     2401 tattgcataa ttattcaata cctctctttg tatatttaca ttatccatat ctagttatct
     2461 atttcaaccc aatattagac tacctactaa taaatttatt ctctacataa ttaacaataa
     2521 ttttgcacta aataacatca ttagcctatc tctgatgcat tttctttaat tttatttata
     2581 gttttaatca atttatttct ctcttcaata tgattttcaa ataaatcaat tatacttcca
     2641 acagttctaa atgggtcttc catcaacact ttgttatcct ctataaatcc attcttgact
     2701 acataatcga ctataagttt aacaaaatgt atttgcttag tattaaaact cttattattt
     2761 aaaaactctg aaaaagcttc atttgcagct tctctatcca atcccactag ttttctcact
     2821 aattgactta ctggagtatc tccaaactcc tttttataat catcacaatt acctaactgc
     2881 tttaagagta tttcctccaa cgtttcaaca tcttgctttg ttatcttttt attatttctt
     2941 aatttaaata ttgctaattc atctttatgg ctatttaaat aatactctac cttcttttta
     3001 taattcttaa gattgtttgc attatataca gaatcatttc tatcttctgc tacaatcata
     3061 tcttcaaaac ttgtataata tatcttttga ctagactttt caagatactt aataagctct
     3121 cttaatgata tacgtacttc ttctacctca aatatatctg aatcttccca aaaatcctct
     3181 gtcattgctt tttcaatagt atatttttgt tgctggatct gtggtatagt cccaagtttc
     3241 gaaagttttt ctgctgtaac cattacatgc cttatccctt tacttgcatt attattttgt
     3301 aagtatgcaa gttgtatatt atacataact acatcaaatc ttttagccaa ttcatcatca
     3361 ctatatgaag ttatgagtgg tgatatatat tctcttatct cattttgtgc tactgctcca
     3421 atattgctcc aaacattttc atttttatac ttatgtacat aacttaaatt catcctaact
     3481 atataactat cctcatttaa agcttttaca gaagttataa gttcttctaa caattctttt
     3541 ctaagttctg catacttacc ttctatatat ttaatatctt gaagttcttt aaccatactt
     3601 acctttatat taaataattt ttcagtaagt gtttctgctt tatttccctc aaatccttta
     3661 ggattaaatt taaaaaattc aaaattatta caaaaatcaa atattaagaa tctttcctta
     3721 tccatatcaa cacctaataa atctggacat aatctagttc ctctacctat catttgccaa
     3781 aacttagtct tacttctcac tttcttgaaa aaaacaagat ttaggatttc aggtatgtca
     3841 attcctgtat caagcatgtc tactgaaact gctatttgag gtaatttatt cttatcagaa
     3901 aaatcatcta ttatagaatc tacataattt atactatagt caactgcttt tacaaagttc
     3961 ccaccatact ttggatacag tttattaaat ctttcaacta ttgccctagc atgctttgta
     4021 ttctttgcaa aaattatagt cttaccaatc ttttcttctc cttctatttt caacccctct
     4081 gtcattagct tatttaaaac taaatctatt gtgtcagaat taaatagcca ctcatttact
     4141 gcattattcc ctatttcgtc tccaatactc tcatcatcat taaatgtatt ttcatattct
     4201 tccttctctt catcagaaag ctcatcatac ttaattccat cttccataat cttagtctta
     4261 aactcaatac tagtataatc tactaaatat ccatcctcga ctgctttatt atactcataa
     4321 gcatatgtag gtactccatt ttccatgtca aaaactgaat aagtattttt atctatctca
     4381 tcctttggtg tagcagtaag acccattaag taagaatcaa aataatcaaa gattgactta
     4441 tactttttat aaatacttct atgcgattca tctattataa ttaaatcaaa atgaccaggt
     4501 gtaaacaatc tctttccatc cttagacttc gtatcatcaa ttgcattcat cattgttgga
     4561 tatgtagaaa atatcatcct agcatcttct gggctgtctt tgctatccaa taaattacat
     4621 aacgccaagt taggtaatag cttagtaaaa ctttttttag cttgctttac caatgctttt
     4681 ctatctgcta gaaataatat atttttaatc caattatgtc ttgaaagtac atcaactata
     4741 gatatagcaa ttcttgtttt tcctgtacca gtagcacata ccaaaagagt tttgcgttgc
     4801 ttattttcaa gagcctcaca tacagcccta atagcttctt tttgataata tctattggat
     4861 atttcatcat ttattgttac agaacttaaa gacttcttac tctttcttct atctatcata
     4921 agttgtaatt ctgatttttt ataaaatcca taaacttttc tatctgaata gtcatcccaa
     4981 atatacatat caaaaccatt cgtcaaaaat attactggtc tttgactata tctcttttcc
     5041 aaacaatctg catataattt agcttgttgt tgaccaattt tcgggtcttt gctcgttctt
     5101 ttcgcctcaa ctactgctaa tggcttacca ttttcaccat aaagaacata atctacatat
     5161 cctacaccac tttcattagg cataccttgt acttctattt cctcccctat atcttttcca
     5221 aattcccaac ctgcaagctt taattctata tttatatatc tttttcttgt ctcaaattcg
     5281 tttacttcgt ctatcttgaa atcgtagttt acagtattaa cttttctctt ttcagttagt
     5341 gactttctta atttctcatt ttcttttatt acttcttcaa gctttttatc ttttgaactt
     5401 aatctctcat aaagattttt aagctcgtct ggtctcgtcc ttttctcttc cccatgaagt
     5461 aataaatctt cattaaagtc ttctgctgta tattcatctg aataacaata gtctatccaa
     5521 ctaacaaatt gatgcaaatt gttaagtgaa agtattgctt ctcctctctc tatgttcgca
     5581 ctagtatgta cagataaatt acctaattta actatatatt ttaataaagg taacatatca
     5641 tagtctataa gttctataaa actattatta tgaatcaacg tactaagatt atcttgataa
     5701 ggcaatacta aatcactatc attagcatat agccatttta ctgcaagctc taaagctctt
     5761 cttgttatag ttgcacaagt agatgggcta actagtatac ttttttctgc ttctaagcaa
     5821 gcagtattaa aactattgaa atttttcttc ttttgtaaaa agtcaaaatt acccaaggct
     5881 tttctccccc ttttgtccct tagttaaaat aaatctctct aaaaactatt tcttacagct
     5941 gtttcttata ctaacaaata cttcttcatt ttctgtaaac aattcgtaca tatcttcaaa
     6001 ctgtggtata tcatatccat gagaatcatc atttctcaat tttctaagtg tttctataac
     6061 tatagaagaa tttttaggta ataaattgtg atactcacaa gctaaaaggt agtcataaaa
     6121 gtttttagat tttatgttta aatcaattga tttataatac aacattgctt ttgttaattc
     6181 taaaaatgag accatatagc ttttaaaacc ttcttttatg tatctagccc ataactcagg
     6241 catttcttcc ttatcatata agattttgaa gttttgcagg tcatataagc aagaaccaaa
     6301 atccctattt acttcttcta ttgagttcca agataatttg tttctaatta ccatacatgt
     6361 ccctccaatc taagatatct attacatcac tgttttcaaa atagaattta tttatataat
     6421 ccttaaaatc tttagattca taaataactt caccttctaa aatagctttt ttaagtgcta
     6481 ttgtataatt atctgtaaca atttttaggt ctacatcaat gtctaatata tttcttagag
     6541 cttctgtctt agttgcaagt tctgtaattg gtactttttt gataggaatc caagctatgt
     6601 ctatatcgct ttctgatgta tgtttatctg ttccataact accaaatatt atgattgcta
     6661 gagtattttc ttttggaaat acatttatta ttgagttttt aatctcattt ggtatatttc
     6721 tcatttaatc acctcttgtt tattaggtaa tattgcttaa ttgaatttat atccataatc
     6781 ttttaatcta tttatacata tttctctttc ttcatcagta aacaactctt tatacttttc
     6841 ttttaatact aatacttcta atgataaatc taatctctta cactcccata gccttgtaaa
     6901 tccttcagta gctccttctt tattgattaa ttctttagca gctaataagg ctcctttgtt
     6961 atttatcagt tgtaaaaatc ttgttggctt atagttacat tgtaaatcag cttctttata
     7021 gatatttacc atgtcatgat ataaatctag ctctatttga ttcatagatt ttattccccc
     7081 attttaatac ttgtatttca gttgaacaat tctcctttaa aagctttttg cattaatgag
     7141 ttaaagttat cttctagttc ttttaagcta gtttccattt caaacttcaa agagtttact
     7201 tgtttaacga agttggcaaa ttgattttga agtctaatag gaggtacaat aatatttatt
     7261 tttctaacag catctttagt tatcgttttt gttacagctc ctcttacttt ctcttttatt
     7321 ttactttcca tataggatgt tagtaaaagg ttatataaga atatatgatt cactttatct
     7381 tcattaaatc taagcataaa ttgattcata gctaaagata ctggtttatt taaaaatggc
     7441 attaaataaa ctttacctgc actacctatc ttatttatta taacctcacc accaaacacc
     7501 tttgattttt caagataatt atatgcatgt tcatcgatat acttaacacc tttttcaaag
     7561 ttattatttt ctaaatctgt agttcttacc attaaggcat aaccttttgt atctaaaaga
     7621 gttacattat ctcttaaggt ttcataacta ccatttgaat gataatcagt taaaacatct
     7681 aaatatttcc ctatttctga aatttcccag tttttttcgt tctttgatgg ggtaccaaac
     7741 atctcaataa atcttgattt aactaattca tccaatactt ctatttgctc ttttctttta
     7801 tctattaact cttgagcctt gtctaaaaca cgcactacct tttcttgcac ttttaaatta
     7861 tacatgcgta tttttatatt agctaatcct tcattattta aagctgattg tgtagcacct
     7921 ttacttaacc tatttttttg atttagaaaa taactactat ttaagtaatg gtataaatat
     7981 tgaggcaata tttcttttga tggttttaaa acataaaagc cagtcgatac tattatattt
     8041 ttattagttt tattaataac tagtgttttc tttgtgtcct tcatttttgc aaatataact
     8101 tctcctatat ttacagtttg atttgctcta gaaggtttat tttcataagt aactatttct
     8161 agattgctta tcttgttaaa atctaagttt ccagtactaa gatatgagcg ttctccatca
     8221 aactttttta cattttccct gcttatacta acaacatcta aaatttttgc ttcatcaacc
     8281 atactctaca cctctaacat tctttctagt tcctctaatc catccactat ctctttctct
     8341 aacaccttaa ccctacctat aataacacta ggagcatcat aaactacttc ctcatacact
     8401 acctctttat acttattaat agataaatca tacccattct ctcttatctc ttccacatca
     8461 acaaaaaagc tttgctctgt tctttttcta tctacttccc catcaagatt atcaaatctc
     8521 ttaataatat cctctatatc actatccttt atctcatttc tcttatcatc caaagaataa
     8581 ccatcagctt tcatatcata gaaccatacc ttatcagttc cacctgtccc tgttttagta
     8641 aatatcatta tagctgtaga tacccctgca tatggcttaa atacaccact tggcattgat
     8701 attatagctt ccaatttatg attatcaact atttcttttc ttatcgattt atgtccacct
     8761 gttgaaccaa acaatactcc atctggcact atacatgcgc atcttccacc aatttttaaa
     8821 actcttaaaa aaagtgccaa gaataataac tctgttttct ttgtcttagt cacttttaat
     8881 aaatctgctg acactgcttc ataatctaaa cttcccttaa aaggaggatt tgctagcacc
     8941 aatgtaaact tatctttatc tgtattaacc tccgataaag aatccttata ctctatatta
     9001 ggattgtcaa cgccatgcaa catcatattc atagcaccta ttctaagcat agttctatcc
     9061 atatcaaatc catagaacat accattatta aaatgctcct tcagcccttg cactaagaaa
     9121 agacttgaat gattatccct taagtactgt tgtgatgata ctaaaaaacc tgccgaacct
     9181 gctgccgggt ctactataat atcttctggt gttggtttaa taagtcttac tatcatatct
     9241 attatgtgtc ttggagtcct aaattgacca ttagtacctg ctgtagccac tttggataat
     9301 aaatactcat acaaatctcc ctttgtatct ctatccttac ttaattctaa attactaata
     9361 ccatcaacta ttttagataa cataagaggc gtaggaattt taaatattgc atctcccatg
     9421 tactttgcat aagctgattc tttatttcca tgtaattttt ttatgaaagg aaacacttca
     9481 ttttgtacta tttcatacat tttttctgag tttccttcat tagaaaactt actccatctt
     9541 aaatgttgat gttcatcatc aaatatcttt tcaaaagaaa cacccaaaag agttgcttct
     9601 gcttcatttt ttgtttctac ttcatccaat cctttaataa ataaaagata tgtaaattgt
     9661 tctataactt ctaatggatt agttaatcca ccagtccaaa atgtctccca aatcttgtct
     9721 actttactcc ttaattctcc tgtaatcata tatgactttc cctccagtgt tcataaaata
     9781 atttaaatat atattttagt cgtacgcctt aatagttagt gtcttatgtt aaatccaagt
     9841 ttttactaca caattattaa attatttttc ccatttacat attttagcta aaaaactaat
     9901 gcaatactct tattttatga ttattatata taccatacaa aattatttta tcatatatcc
     9961 atcatagaag tcatctacct ggataactct taccatatta taacttttta atattaattt
    10021 aaacttagaa agaaattttt tatttaatta tatcatcttc cttatttcaa atttctcaac
    10081 tacaatccta atcaaaaaat aaaataagat gctgtctcaa aatagatata atcttctttt
    10141 ttattgtcag aatgatgaaa atatatttga tttcacacaa aaaagagtgc ctcatgaact
    10201 aaaaagttca ttttgagaca ccccttaatt taatcttact tattctttcc ctttattatt
    10261 aacacttcac tgtccctatt gcttttccta cacatccaca aacttccatc ctactcttaa
    10321 caaaatcttt aactgactct aaaccttttt taccatattt cttagggtca attacctctg
    10381 ggtcagaatt aagcacatcc ttaaccccat tgctataagc aatccttaat tcagttgcat
    10441 aattaacttt gcaaatacct ctttttatag attccttaat aacctcatct ggtattccag
    10501 acccaccatg taatactaat ggaactgata caacctctct aatttcagat aatctatcta
    10561 aatcaagctt tggctctccc ttatatagtc catgtgccgt acctattgct actgccaaag
    10621 atgatacccc tgttctctct acaaactctt ttgcttctaa tgggtctgta tatgctccac
    10681 catcaccacc atctaaatcg tcttccttac ctccaacttt gcctaattca gcctcaacag
    10741 gtatatctga aggcatacat gcatctacaa cagatttaga tattgaaata ttatcttcgt
    10801 aactttcatg agaaccatca atcataatag aagtatatcc aactctaagt gcctgcatag
    10861 ctagtccaaa gcttgaacca tggtctaagt gcattgccac tggaacactt gccttctctg
    10921 atgctacctt tacattcgct aaataataat ctagccctgc atactttatt gttgaaggtg
    10981 ttgtttgcaa tattactggt gatttaagtt cttcagcagc ctctattact gccataacca
    11041 tctccatatt ttcaacatta aatgcaccta ctgcataatg cccttcttgt gcatctaata
    11101 atatttgttt agttgtcact aatgccataa tttctcctcc tatatgttta tactgatttg
    11161 atttcttcgt tatttaattt tgatactatt tctctacttg ttttgctatt taataactca
    11221 tttaaaaatt cttcattcat taattttgaa gatagttgag ataatgtttt taagtgtaaa
    11281 ttatcaccat tatctgtagc tgatatcata aatataatat gtgccaagct tccatcttct
    11341 gattcaaagt caaacccttc ttttgataca cctactgcta cacgtggtac ctttactgca
    11401 tctgtttttg catgtggtat cgctatcccc ataccaaaac ctgtagatga tatttcttct
    11461 cttttaagga tttcttcttt aaacttcttt ttatctaaaa gaacaccaga tttatcaagt
    11521 attccaatca tttcatcaat tacatctgtt ttattttttc ccttaacatc taaaattact
    11581 gtttcttcgc tatataaaga gatattatta gatacgtttt ccgattctgc actttcattt
    11641 ttatgtccat cttttgttgc atttgtattt tctatagtat ttttctcatt tactacttta
    11701 ggtgtaacca tcatataaag tacagcacct actaatgaac ctacaaatat acaccctacc
    11761 cataaaagtg gcttattgac taatcctaga accaaaaacc ctccatgtgg agctggtact
    11821 tctattttca ttatataagt taaaattgat gatattgatg atgcaatcat aagtactggc
    11881 aacactttta atggattttt tgctgcaaaa ggtattgccc cctctgttat atgagtacta
    11941 cctagtatat aattgattaa tcctgctgct ctatcttctt ctgtaaatct atttttaaat
    12001 aaagtagatg ctattgctat taccaatggt ggtgttatac aagctgcaga tacacctgcc
    12061 ataaataaat aatttccctg tcctagaagc atagttccag ttacatatgc tgctttattt
    12121 actggacctc ccatatcaaa tgctgacata catcctatta ctattcccaa aattatagga
    12181 cttgaatttt gtaaccccga tagaaagttc atcatagcat tgttaagtgc tgaacatggt
    12241 ccaccaagta acaacattgc tacacccaca agacctacgc tgacaatagg aattataaaa
    12301 atcgatttaa gaccttctaa ttgtcgtggt aattttctta acaaatgtac aaatagttta
    12361 actaagtaac ctgctaaaaa tcctgatatg attccaccta aaaaccccga acctgtatca
    12421 aatgatatca ttcctccaac aagtcctgcc aacataccag ctcttcccga tatagattga
    12481 gctataaatg ctgccaatac aggaaccatc aagttcatag aatatccacc aacagatttt
    12541 aatgtagctg ctatttgatt atattgttct gaatttgggt caaatgaata aatcccccat
    12601 aaaaatgata tagcagtaag tactccacct gctactacta aaggtaacat gtgagataca
    12661 ccattcatta agtctttata caacttttga ccaaacgata aatttccttc ttctgcaatt
    12721 ttactgcttg ataagtttcc ttttctaata gctacttttc catcaataca cctttgtata
    12781 agttcattag ccttatgtat accttctttg acaggtactt ctattaccgg ctttccattg
    12841 aatctgtcca tatcaacatt tttatcacaa gcaactatta ttgcatctgc catttcgata
    12901 tcttttgctg ttaacttatt ttctacacct atagctccat tcgtctcaac ttttatgtca
    12961 caccctaatt cccttgctga attttttaat gcttcttctg ccataaaagt atgagctatt
    13021 ccagttggac aacctgttac ccctagaatc attttctttc ccatctttat accacctttc
    13081 tgactcttat attttttata taatcttcca cattctccaa attacctata ccattacttt
    13141 cagccacact tgctcctgtt gctgctgacc tttttaatgc ttcttcaata tgttctggat
    13201 tttccaacca tatacttaaa aaagcagcta atgcagaatc accagcacat gcagagctga
    13261 ctactgtaac tggttgagca ctagcataat aaattgattt tccattataa aaataagaac
    13321 ctttttctcc taaagttagc aatatatttt gagcaccttt taaatgcagt aatttaagta
    13381 catccttaat atcttcttca tctcttacaa taattccaaa tatatctttg atttcttcat
    13441 catttggttt tatcaaatat ggattatact gtagtaactc ctttaatttt ggtgaactta
    13501 tatcgagtat tgtctttata cttttatttt tacaaatttt aaatatatcc tcatagtaat
    13561 tatcatcaat accaggaggt agacttccac ttattgataa gtaacttata tctgctaatg
    13621 actgtatctt atctagcata tctagttttt gtttttcgtt tacatatgac ccagagttta
    13681 caaatttaaa ttcatcactt ccatcattta aaaatatatt tatacgagtt gtatcttcta
    13741 cccaagtagg taatacgtca aatcctctct tttcgctttc ttcaactata tattttcctg
    13801 aaaatcctcc aaaaaatcct aatatcttag attctatacc ataatgacct agtacaaaag
    13861 ttacatttaa tccctttcca ttaggagaat atacagcatt tgatgtacga tttactaatt
    13921 ttctctttat cccattagta gatatattca tatctattgc aggattcgta gttaatgtat
    13981 atatcattgt cttccccctt gcttgtgctg tgtatgtttt cattataata aaaaagtcta
    14041 tgaaaatctt ttcacaaata gaaaatcgat tttcatagac ttttcaattt aattttcaga
    14101 attattatat ctgaccatat aatctataat tatcctagat ataatataca agggcaatct
    14161 ggaagatact tcatattcag aaattgatat atgttcatgt ttgtatccta caatatataa
    14221 atccgaatac tttataagtt ttgcactttc actacaacag caagttataa ccttagctcc
    14281 aactaaattg gcattctgtg ctgattctac aagctctatg gtatttccat tcaatgaaaa
    14341 tatgattata agttcgtctt tttttattga tttagatttt attttcatta tgttggggtc
    14401 atttataaaa acaacattaa aatcaagtaa ttgcaattta agactaaact cttttgctac
    14461 atattcagta agccctcttg ccaaaatata tatcttacta gatttattta tagtttttat
    14521 tatattgatt agtgtagtta aagatatttg ttctataact ctctgtgctt ctataaagga
    14581 tttatttatt atatccccca tattttgtat atcatcattt tcatctttta ctgttaatct
    14641 atacctaagt tcattgaatc catttaaacc acattttcta attgctcttg aaacggttgc
    14701 tggagatgaa tatgtttcat gagcaatctc tactatagat aattctggta atattgattc
    14761 attttcattt ataaatttaa ttatttcaag ttccgtcttc gttagatttt cagaattact
    14821 tttgtttagc tcaattatca tatgttatct ccccttatat aagttttatt tgtctaaatt
    14881 tatcgagtaa tataatatat tacctcttaa ctacttattt ttatatacaa ttaagattta
    14941 gtcaatgtct atatatgtaa atttataata taataatttt atgcttcaat taattatgta
    15001 attattagag tattttgtaa taaaaattgg agttactcaa ttactgaaac tgagtaactc
    15061 caattcttac agcctcaaat taactcgaat tattgaaaat gattcataaa cattatccta
    15121 tactctcact tatcaattta tatacttgag taataccttt ttttattaac agaaatcatc
    15181 ttactaacaa ttgaaattgt agttccagta cataatattg aaaatatagt tccaaaccct
    15241 aaatttccgc ctaaatatac acctaaaatt aaaaatatta aatctgctat agtttttaaa
    15301 actttatatg gatacttaaa tttgccctca atatagatta atatagcatc taaagggctc
    15361 attccaatgt caaattttat aagcattgca aatccactac acataagtat atgtccaatt
    15421 ataaagctca ttattctaat gataatccct aaactactta tattaaaatc taataataaa
    15481 tatatataca aatctataaa tattcctgct actaatacat aaacacagct tcctaatccc
    15541 aaactttttt tattcaataa caatgctata agtaatagag ttccattata tattagccct
    15601 gctattgtat aacttatacc taactttata tgtataccct catttaaaag tgtaattgca
    15661 tcactaccaa ggcttgactg aagtataaga gatataccac atcccagtat atttataatt
    15721 aaaattacaa atataaattt ttttatgaca ttcataatta ataaaccacc ttatttataa
    15781 ttataatccc tcttataaaa ctgtattaat aacatagtat ttataagagg gattatttat
    15841 atttaaaaac tatctacttt ttcttttaat tcttctccat tagttcttat tacattttta
    15901 taccaataaa aactatcttt tttgtaacgt tctttgcttc catttccatt gttatctata
    15961 tctacatata taaatccata tcttttttcc atttcttgag atgaacaact tactatatct
    16021 attggcgacc acatacaata agctaatatt tcggttccat cagctatagc ttcacctatt
    16081 tgttctatat gttttcctaa ataatctatt ctataactat catgaacttt tccatcttca
    16141 aatttatcat gcattccaaa tccattttca gctatcatta ttggcttttg atatctatca
    16201 taatattgag ataaagtatt gtataatcct ttagggtcaa ttgcccaccc ccaaggattt
    16261 gcttttaagt attgattttt tacattatca attggattca tggtattttt acttgctgat
    16321 actgttgtag agtaatagta tgatagagct agaaaatcca tagtattctc ttttaacact
    16381 ttatcatcac cttcttccat aactatattt atgttatttt ctttgaagaa tctcttaata
    16441 taagctggat actcccctct aaaacttaca tcagtaaaaa aatattgtat tctatttctt
    16501 tttaaagcaa atactacatt ttcagggtcg caatcttctg gataagatgt acaatctgcc
    16561 atcatcatcc ctatcatcat atctgaattt atttcttttc caagcttctt agttaaagct
    16621 gtagccatga attgatgatg aatagcttga tatttggcct cttctacatc atctacctgg
    16681 tctacacaaa atgcaactga attaaatgat tcatgataca ttaaatttat ttggtttata
    16741 actatccaat atttaacttt atctttgtat ctatttaata ttaattctgc atatctaaca
    16801 aagaaatcca ctacttttct attagaccat cctttatatt ttaatactaa attcaaagga
    16861 gtctcataat ggagtagtgt tacaataggt tccataccat tatctattac ttcatttata
    16921 agattatcat aaaactttaa tccctcttca ttaggctcta aatcatctcc atttggaaat
    16981 attctagccc aatttattga aaatctaaaa cactttaacc ctagttcttt caaaagcttt
    17041 aaatcttctt tgtatgtatg ataaaagtct attccattac gcttaggata atacccatta
    17101 ttatctttca tagcaaactt tattttatct acagtcatat cagactcgta atgtttagat
    17161 atatctctat ctttatcata catatgtaca tcagctaagc accacccttt gccaccttca
    17221 ttccaagcac cttctaattg attagctgct acagctccac cccacaaaaa atctttagga
    17281 aacttactca atttaatcac tccttatttt atttatttaa atcatttact attatttacc
    17341 ttatattcta tagactttac tattcctact aaagacatag ttatatttaa ctcgctgaat
    17401 attgtcataa gagtatcttg agcatgtgca aataaaagtg aatgtgctct tttttctcca
    17461 ctagcttccc cttgtataat gttagtttga gctctatgtg cttctgtcat aaactctttt
    17521 gctttagcta gttttaaatc agcattttta taatcaaacc tttctaaatc attaagagca
    17581 tgtttaattt ctgttctagc ctcacctgca cgaagtatta tttgcattgc tacttctact
    17641 aattcttcat ctattacatg ttcttcagtt gtcatattta taatttcctt tcaacatcta
    17701 aatttaagct tcttgactct ctaattttag ttcttctttt tcataagctt taaaaaatgg
    17761 ataccatatc atgaatagca atactatatt tattgctaaa acaataagcc ctctaacatc
    17821 ttgatttacc ataaaagtag atataccaaa aggaagatac cataaattaa acaccttact
    17881 cggaattgtc acaaatccca tactcattga taagtaagta gttattgata ttactactgt
    17941 tcctatccac ataggtaaca ttaaaaatgg gttccatgct actggagctc caaacattac
    18001 tggttcattt atgttcatta tagaaggaac gatacaggct cttccgattg cttttattct
    18061 tccagctcta gataaacaca tcattagcac tagtggtaat gttgctcccc acccacctaa
    18121 agttatccaa cctgtttgaa caacttcata agtatatata ttagttgcaa gccctcctga
    18181 tgctacagct gctgcattag ctgcaattgc actaagctga attgcataag ttattgggga
    18241 taatacccat ccacttatac ctaaagaata taaaaatact actaagaagt ttaatagtac
    18301 aaatccaggc aatgtttgtg ctatatctga tactggttta aatatcctaa ctattatttc
    18361 aaacatatca aagttaagtc cataaattag tccccaacct ataaacatta ttactgctat
    18421 aggtaacata ctatcaaacc acttacttac aaaatcagga agtgctgaat cttcaccaaa
    18481 aaatgagaaa gacccaaata aagacattat taatccggag aataatcctg caactagtgc
    18541 tacaagcatt ccttctcctc caaatctatt gaagttaaat atagctcctt cattcgtaat
    18601 ctctggacga atcatcatta acattaatcc agcacctgca agaccagcta ctacactcat
    18661 actttcaaat ttcttattct tcattatttc cataggtaat aaaaatgcta taaatagtcc
    18721 taaaagtcca aatgtatatt gatttattgg acttaaatca ggtaagaaac ttatgtaatt
    18781 cttcaagaca gaaactatag ttactaaaga acctacaaat accataggca aaactttcat
    18841 tatagttgat tgaactgatt gtacccatat attcttagtt atcttattta caacaggagt
    18901 aaacttttca cttaacaaat ctataaattt attcataaac aatctcccca tagatatttt
    18961 atttgtttaa tattaattct aatgcttctt ctaaagcctt tacaccatct aatcttccat
    19021 atgctgtttt agatatacat gctactggaa cattatattc atcagctttt ttttgtattt
    19081 catctttcat ataatctaaa tgtggtccta ataataaaca atctatttct ggtaaatatt
    19141 tatctatctc tgactcactt ctagctttta tatctgcttc tatgcctttt ttctttgcac
    19201 tctttctcat actagttgcc ataaaacctg tacttgctcc tgagccacat gccaacaaca
    19261 cttttatatt cttcataaca atacctccag tatttttatt cattaattta tttcttaatg
    19321 aatttttatt tgatttgatt gtatttatat tgtacttcct aaataatttc ctttcacata
    19381 aatatatttc ctaataatag gaaatcattt actcttaacc agacacataa atatatagta
    19441 taatgtacat tataatacgt acattcaatt atataaatat catatcatac agattttatt
    19501 ttaaaactat gagagggaat ctaatgaaaa catctaaaaa agagttattt ttaaacttca
    19561 taaaaacgca acatgattgg atagattcat ccacattagc taattatctt aatgtatcaa
    19621 cacgttcgat tagaaaatat gtaaatgaaa ttaattctac tggtgaattt atattatcct
    19681 ctaaaaaagg atataaagta aatctcaata ataactgtca aaccaaggtt gatagttcag
    19741 aaaatatatc acctgataac aggttaaatt taattttaaa agaacttata gttaatagta
    19801 atggaattaa tatatttgat ttaagtgaag aattgtttgt tagtccagct actattgaag
    19861 gggatatagt caaagctaat aaatttatag gaagttataa tttaaagata aagcaatcga
    19921 aatttttatt gaaactaatt ggaaatgagt ctgcaaagcg caaattaatg agctctataa
    19981 tatttaaaga aacgggaagt gattttttaa gcttatttga tgtacaaaaa atatatcaag
    20041 agtataattt gaccaaactg aaagaaaata taatatatat attaaaaaaa tacaatttgt
    20101 ttataaatga gtacgctata aataacatac ttcttcattt aatgataact atagacagaa
    20161 taaaaaagaa taactacata gaatctgtcg aagtagtaaa ctacatagat aataataatg
    20221 atataagcat ttcaagagat atatcaaatt ttttagagtc tgaatacaat ttaactttaa
    20281 ctagtgcaga attatactac ttagtatttc agttaacaaa caaaaccaca gtcttaaatt
    20341 atcatgaaat ggatactaag tcattgccaa attatataga tgaacatttt gttaaattaa
    20401 ctaaaaaaat aattaaaaat gtatatgact tatactttat agatttatct gatgatgaat
    20461 ttgttgtaaa atttactctt catgtaaaaa atttaatcag tagggctaaa aataatcaag
    20521 ttttgagaaa tcaaatacct cagaagttaa aagattccta ccctctgata tatgatatat
    20581 ctgtttatat ttgtaatcaa attcaaattt tagaaaacat agaaatagat gaagatgaaa
    20641 tatcgtatat atctcttcat gtaggttcat tttttgatag acaaaaatta ctagaagata
    20701 aaattctctg tgcattaatt actccaaatt actatgattt acaattcaaa atagtaagag
    20761 acctcgagaa gagatttaat gaatccatag aaattattca aattttttca gatactcata
    20821 atcttgattt tgacaataag gtagacatgg ttattactac tttaccaatt agtgatagat
    20881 gccctatacc ttttgtttat gtaaatcctt acctaaacag aaaagattat gataatatcc
    20941 aaagtaaatt tattcagatt aaagatagaa aaaatatact tactgtacaa aatcatttag
    21001 aaatgtactt tagtgaatct ttgtttatga aaaacatata tttagatagt gctaaagatt
    21061 acataaaatt tatgggaaat attctgtaca aaaataaata tgtcaaaccc aattatatag
    21121 atgatgttct tataagagaa aaaatgtctt caactgcatt taataacaat gtagcaattc
    21181 ctcatagcat gaaaatggat gctttaaaaa ctggagtatg tttaattgtt aacgataaac
    21241 cagttaaatg gggtgaggaa aaagttcaaa ttatagctat gatacctata aatgaaaaag
    21301 aaaaagaaaa attcaattac atatttgaaa gctttattga aatattatct gagtggaata
    21361 atgttaaaga acttactaag gcagataact acagtagttt tatgaataga atagcttatc
    21421 ttattcaaaa tatctaaatc cattaattta tttccaatat atgtttctta agttattaac
    21481 acaaaataga taagctgtca cactaaaaag tctaaattaa tatgtgacag ctttttacct
    21541 aatatacttt gctttaatac tacatatttt ttgattgttg tatactagtt ataagattga
    21601 atatttgtgt tgttgtagct gtcatattct cactagcagt tgctttatcc attgcttctt
    21661 ctattgtcat agctctattt actatagaaa aataagcatc tataccctct tcattacatt
    21721 taactgcatc ttctgttgtg gctccagcca aacctattac ttttacccca tatttttttg
    21781 ccagttttgc aactcctatt ggagccttgc ccatagcagt ttgattatcc aatctacctt
    21841 caccagttat gacaaaatct gcatctttta actcttcctc taatttaatt tcttctaata
    21901 ttatttttat tccagattct aattttgaat ttaaaaatgc taaaaaagca aaacctaatc
    21961 ctcctgctgc tcctgctcct tctacattag ctacatcttt acttaagtaa tttttaacta
    22021 ctttggaaaa atttctaagc ccattatcta actcctcaat tatttctgat gttgcacctt
    22081 tttgagaccc atatatgtat gcagctccat ttttcccaaa cagcggatta tttacatcac
    22141 aggctatttt aaacttacat tcatctaatt cctttaatct attttctatt tttatatgtc
    22201 ttatttcatt taaaactttt ccacctaatc ctactaactt gttattttca tcataaaact
    22261 caaaccctaa ggcttgaagc atacctatcc cagcatcatt agtagcactt ccacctattc
    22321 caactataaa atttctatag cctttattta ttgcttcctt tattatttcc ccaacaccat
    22381 aagttgtagt gtataaagga tttctaagtt cagtcggaac caatgttaat cctgatgctt
    22441 gagctatttc tattattgct gtacttgtat tttttaataa tccatatgtt gcatccactt
    22501 ttttagctat tggtccagta acttctatag tctctttttt accattcatc ccttgaacta
    22561 aagcatctac tgtcccttca ccaccatcag caagtggcaa tatctttact tgcgcatctt
    22621 ctttaacctt caatatcccc ttttttattg catttcctgc ctctattgaa gataaacttc
    22681 ctttcaaaga atctatagaa attaatattt tcatatttat ctactccttt ttatttactc
    22741 tttattttaa atatataatt ttataagctt aatttatctt ttattttata ctctatattt
    22801 cactttgtat acaacttata cccttaatat tcttttatct tataaaaaag ggagtgtctc
    22861 aaaatgaact ttttagttca tgagacactc tttttgtata aaattaaata tatgtttatt
    22921 atttcaacaa tgataaggag gattatctct attttaagat aattcctttt tagcactatc
    22981 taatcatttt tagcactatt taaccaatat agttttaact tggttcatta ggcactctac
    23041 atttacagag ttattatcta ttgatataga ttctaattct ttttcattta ggttagtcca
    23101 tttagctgtt ttaatatttt tgttaaatac tacgttagct gattttgttt cttctttaga
    23161 attgtaaaat ctaactacta atgcctcttc tttttcagcc ttttttacaa cacttaacac
    23221 aaggtcctta ttagtttcct taagtaaact atatgaataa tctacttcta tttctgattg
    23281 attcaatttc atagcattat atggtatttt attataagtt tgtatttttg ttaaatactc
    23341 tttagccata tttgcaacat ttgcttctag tgtagatttt tcatgagtga ctattgctaa
    23401 gtctattttt atatctccta tcatttgaga atctggagta ggcaacttta ttcctgatgg
    23461 acgacctggg cgtcttgcca tttcttcttt acctaggaat cctacacttc taaataatgt
    23521 tatagctatt gtgtcaaatt ttttccctac tatttcaaac tctcttgtac tgtttgtaag
    23581 aacagctact ccatgctctt tgtcagacaa tccaacgaat gttagcattg gatatataga
    23641 atctggtctt tcactccatt cttcctgttg ccaaacttcc atagcatcat catacacact
    23701 tctctttata tgtccaaact gattatcaga cacagaaaac tttgaagcta tatttgttgg
    23761 tatataagtt ctaaccctac aatctcttgc ttggttattt atattgaata ctacatttat
    23821 tatagcttta tcatttggaa catttataac tatatcaaag tcaataaatg aatctgctct
    23881 attatttttt ctagcttgta tatcttttgg tacttttaat ctataattta tctttatctc
    23941 attattaaat ctatttttct ttatagtgga atttgctact atattatcgt taaatattaa
    24001 tttctcattt ggaagtggtg aaaaatcata ctcatctcct tcatctcctc cattttccat
    24061 caataataca ttttcaaaag ttttatttaa ttttttatca gatatcttta atgtcccgtt
    24121 ttcattaact tgaatagtat aaaagtcatt atctaccatg tcacatactt cataagcttt
    24181 ttctaacata tgttcaactt cttttatcat atacgcctta taccccattg caggtatttt
    24241 atctttaaag tttattgtat ataccataaa tgaatcatag tttccataat gaacaatttg
    24301 acggtctatt aatcctgcat ctattacttc tttatgtacc acttcaaaat ccaacttatt
    24361 attatcttta tcaaatattt caaatgcttt cattttagtt ataacttggg cgttcactat
    24421 ttcattccta ttgtaaggca ttaagttaaa tgctactaac ttatctaaag ccatttcaca
    24481 actcattgca tcaactattc ttcttttgta aaatgttatt aattggtcta ctttttcctc
    24541 agctaagaaa aatctattca ttatttcctg gtgaacttta tctgaacaac aacatccaat
    24601 gctgtcatga gcatgatttt tcattatttc tttccaaatt aactctatta atccatgatg
    24661 atattcaaat cctagagaat atgcaataga agctagtggc tccaatatgt tggttatctt
    24721 tgattctatt ctagcatttg ctgattttat atccattctt gttgagaata tacttctatg
    24781 aaccctcata tatttcccat ctaaaaattc cccttttatt gtatctatat ccttattttt
    24841 ttctagctct ttaaatatgt tttcatatct acttaagaaa aattttctct ctggataaca
    24901 ttcttttaat ttatatataa cttcaaatat attcttttga attggcattt ggtcatgtcc
    24961 atttggaagt attatatggt cagtagttgc acccttatca agaaccggta aatatttatc
    25021 cattcttgtt cttagctcat cttcattcat tggtagatat tttcctattg cataacctaa
    25081 tggaagtatt tgaaccaaaa cagaagaccc atcatcactc ttccaattaa actctgtttt
    25141 attagttcct tttctttcag aacatcctct ccaaaacatt gagtatttta tatcaaaccc
    25201 atttagtatt tgcggcattt gagcactttg accaaatgaa tctggtaaat atcctatttt
    25261 catatattct ccaaattcat cacaatcttt tattccatac agtaaatttc taagtatgga
    25321 ttctccacca actaccattt catcagtttg agtataccaa ggacctatta ttagccttcc
    25381 ttcttgaact aattttttta ttctctcctt acattcaggc ttaactgcta aataatcttc
    25441 taatatagca gtttgaccat ccatcacata atatggatag ttatgattat tttctaacat
    25501 atccattatt tcttccatat tatttacaag taatattcta gattcctcag ttgagaagta
    25561 ccattctcta tcccaatgca tatgaggaac tatgtgtaca ttttttttca ttgcttatcc
    25621 tccaaaactt atctgataat attttctgtc taattaaaaa tccaataaaa atactactga
    25681 gtttacacaa ttttttaata tttattttat cactataatt aaacctttga tttaaatatc
    25741 taataacgta tcttttaatg tatctttctt tgattttttt gtttcctagt tattattaat
    25801 aatattgctg atattatagc tccaagtact gccgcaaaga accatattga tgctataata
    25861 aatcctgaca tacttccctc taaaagcgcc aatgagaata tccccgctcc aggcacttga
    25921 agacctatat tgaagaatgc tactatagac ccagttatgg ctgaaccagc tactaatgat
    25981 ggtataacat ggattgggtc ctctatcata aatggtattg caccttcagt tattccagcc
    26041 aatcctaata tccaagtttg ctttcctatt tccttttctg attctgaaaa taaatctctg
    26101 cctaaaacag tagctattgt aactccaaaa gctgagacca tcttaactga tgcaaatatt
    26161 gcataaggta taaaatttcc acttgctatt gctcctattg caaatgtata ggctgcttta
    26221 tttattggtc cacctaagtc aaatgaaacc atagctccta ttattgcacc tagtagtatt
    26281 gcatttattc cagtcatccc atttaaccaa tttataagac cattgttaag tgctgctaca
    26341 ggctttccta ttacaaataa catcaataaa cctactacca aagaaccaac tactggatat
    26401 acccaaaaac ttacgaatcc agctaatgtg cctttggctg gtatattctt ctttaaaaat
    26461 tttactatat aaccagcaag taaacctcct aacatacctc caagaaatcc actacctatt
    26521 aaatcagctg ctagacctgc tgcaaatcct ggtccaagac ctggtttttc agctagtgag
    26581 taagacatat atgctgataa tactggaacc atcaatgtac ctaacatccc tccagaaagc
    26641 tttcttaatt gccataacca agaaccttct tgattgtata gttcttgtaa accaaatgct
    26701 tgagatgcta atactgcaaa tgcaagtatc atacctcctg ccacaataag tggtattata
    26761 tgagatactc ctgtaagtat tgctgatttt acatcttttt tccatgatga tttttcacaa
    26821 tatgatatat cagatgattt ttctatataa tcacctttac cttgtttttg tgctacttct
    26881 aaagcttcgt atattatttc ttttgccctt tttagtggag ctgctactga cgtttttatg
    26941 tttggcaaat gttcaaatct ttctgtattt ttaggtgcta catctgttgc atatattatt
    27001 gcatctgcct tttttattat atctggtgta tgagggtcca ccataccatt agctccttgc
    27061 ttttctacat acacatccac acctatttct ttgccagctt taattaatgc ttctgctgac
    27121 atatatgtat gtgctatacc tgcagggcat gctgttattg ctaatacaat tttttttgat
    27181 tcattttgaa tcctaatctc ttcttttatt tgttctttct tttcttttat atctaagtta
    27241 ttaaataact ctttcttagt ttttgaattt aacaacatat tcatataatc ttcattagac
    27301 agtctagtaa ctaactcaga aagtatttgt atgtgtgtat ttccaccttc actttctggt
    27361 actgctatta aaaatattag ctctacttta ttatttgagt ctacgctttc ccaattttct
    27421 attggtgtct ctaaggtcat agctgcaaat gcagcttctt taactgactt agattttcca
    27481 tgaggaattg caagccctct ttcaaaacct gttggagata aagtttctct gtacattaca
    27541 gcttctaaat attcctcata agatgttaac ttgccttcat catatagctt tcttgctaag
    27601 aactttattg cgtcttcctt tgtttttatt ttttgtttta aaaaaacaag gttttcattt
    27661 gtgattttct taaattccaa aattacgacc tccttaaatc atttaatgtt atgaatatag
    27721 cataaaataa atcatgaaaa tgtttttttg aaattataca cacaatttct tcttaaaaaa
    27781 taaggatttg tgtatttata cacaaatctc tatcttaacc agtattgttc tattattgct
    27841 ctcataatca tcataattgg aaccttatct gatatattgt acccatttac ttgtttaatt
    27901 gttgaatagc aatataaact tatatctgca atttgttgca attgattttc acatatatgt
    27961 gttagagcta ctaccttaca acccttatta actgctgtct ttgctgcttc tataacttct
    28021 ttcgtttctc cacttaaact aagcgcgaaa aacatatctt gttcacctaa ttttcctata
    28081 tcatgaagta cttcatgtct ttggctatag taagttatat tatcatgccc acctctaatt
    28141 tcttttacta atatctcaca taaaggaatg ttttgaccta ttccataaac tataatactc
    28201 ctagattttt gaattatatc tgctacttta tttatttttt cataatctat tagctctaat
    28261 gtctttttta tatctgattc aaaaccattt tttggctctt tatcctcatt ttttaactct
    28321 tcttttaaat tgttttttag ttgagagaaa ccatcatact ccattttttt acatagccta
    28381 actatggtat ttgggacagt aaaaaattta tctgctaaag attgtattga taattttact
    28441 atttcttctt tgttttttct catatactca attatcatgt cttctgtatc tgtaaattta
    28501 tactcatatt tttttgctct ttcataaaat tccatagttt ctcctccatc tattttatta
    28561 ttcataagta aaattcaaaa atattagcta aaattttcgt taatataatt ataatttaat
    28621 atttttaatt tattaatgca tactactctt tttattatac ataaaaaatg tgtacttcct
    28681 atattgatag agatgattca ggtttttatt ttgatacatc tctatcaata tttttttaga
    28741 aaatacacat ttttttcaaa tacttatttt taattaaaac tttaggtaat ctattcttta
    28801 tatataatta ttagacttga aaatatcttt aaaatcaacc aattcaatat tatttctttt
    28861 taaccacatt ttaacttcct tatcacatag cattgcatgt tcatatactc tctcttgaat
    28921 aagagaagat gattttataa gactataatc tataaatcct ggatgagtaa ccatcaatgt
    28981 tatatcttta tctaaattta tatatttttc ttcaaataat ttaacaaaag atttatttaa
    29041 cttgtaaaaa tcattctgag gatatttact tggcatgcta atacatgaat ctaaataata
    29101 gggacaactt ggtataccgt attcttttgc agcatcacac attgctgata ttgatacatc
    29161 ttcttctata ccatgtatat caatatactc aggcaattta ctcatataca acttgtaagt
    29221 ttcaatttga gcacaaactt cttcatatac ttcttcataa ttaaaaacct ctttacctgt
    29281 cagtagttgc gttcttctta ttttgctaga aacaaatttt ccaccttcat ctaacaaatt
    29341 ctttatctta atatgttctt tacaacaagg ttctcctatt actaaattaa catgtaaacc
    29401 aaaacataca tcatcatttt ttaccatttc tatagcttgt cttgaaaaag gcatattcac
    29461 catgattcca gcacttgtaa caataccgtt cttataagaa gatattatcc ctagtgtaac
    29521 accttctgaa tatcctatat catctgccct aactactaat ttcttcattt tatgcatctg
    29581 cctgctttct caactcatca agttgcattt ccatctcatc ctttacttta acaacatccg
    29641 ttcctatgat gatttgaact gatgtattac tcaatttaat tacccctttg gctcctgttt
    29701 ttttaatatt atcatcattc aaaatagtgg catccttcac ttctaaacgt aacctcgtca
    29761 tacaattatt aagagtcact atattttcat aaccaccaca atttttaatt atctgcttag
    29821 ccatatatga aaaattacca tgagaaagtt ttaaattctt ttctttttca ctaactttat
    29881 cacctatctc tatctcaact tcatgtcctg gtgtttgtac attatctttt attattatat
    29941 atttaaatgt tacataatat aatacaaaga atacaactcc tacaggtata actaaccacg
    30001 ctttatctcc ataactaaaa cttaagacat agtcaattat acttgctcca ttaaatgaac
    30061 ccaagcgagc acccagtaaa tatagtacaa ctccacctag accagaataa aaagaatgaa
    30121 ttatatataa ctttggagat gcaaataaaa acgaaaattc tattggttca gttactgttg
    30181 ccataaaaga agtcaatgct ccagcggtca ttgcaccttt tacttcttgc ttctttttat
    30241 cttgtgctgt tttatatata gcataagaaa tagcaggtat tgaaaacatc ataattatgt
    30301 aaaatcctgt ccagaagaat cctgcatttg ggtctcctgc cataaatcta ggaatttctc
    30361 ctcttataac ttctcctgtt gatgttgtat attcacccat ttcaaataat atgtaagcat
    30421 ttaaaacttg atgtaaccca gttggtatta gtgcacggtt acagaataaa aacaagaata
    30481 ttcctattgc accagctcct cccaaccaaa aaccaaattg attcattgca gcttgtggat
    30541 atatccataa taaactaaat atatatgata aaattaaagc aaccactggt gatagtgtaa
    30601 ttacacattt ttctcctgca aagaagctta aaaatgctgg aactttaact ttatatgttt
    30661 tattgtaagt ccatgctata caagctccaa caattatccc accaaataca cctgcattta
    30721 atgtatcatt caataactct aaatttgact taaatatcat atatgcaact acggctgcaa
    30781 ccattacact tgtcttgtct ttagactttg aataagcact aactgctcct aataaaaata
    30841 agtagtccac atttgaaaat actatattag atatattaga tatagctggc acattaagca
    30901 tatttggacc aaataataat cctaatatac ctgctgctgg cattacagct attggcacca
    30961 gcatagtctt tcctaaaagt gacagttgtt gcatcatttt gtttaagaac ctcttcatat
    31021 ccaatcccct ttctatttcg gtttaatttt ttgtgaaaac gatttattca cattttatat
    31081 tttaaatatt atatttgttt aaacaaattg tcaatatttt ttattcattt tatttttatc
    31141 atttagtttc atatctaaat ccttttaagt atattgctat atctagccta ttttttaaat
    31201 ttattttttt tatttttaaa atatgtttaa ccatgtattt gaaacaaccc acaaaatagt
    31261 tcaaatttat cctttttatg gtttatataa ttctattttc actatacatg tctaaacaca
    31321 tttgatataa tttgttcata tcagttcaat aatattacta aagaccttta aggagggata
    31381 ggggtatatg gctaaaaata aattttcaga gatatatgaa gtattgaaag aggaaatact
    31441 tgatggtaaa tatacaagta atatgatgct gcctactgaa cttcaactta ttgaaaggtt
    31501 ttcatgtagc agaaacactg tacgtagagc aatttctcaa ttaaacacag aaggatatgt
    31561 tcaaagtata aaaggcaagg gtgttgtagt gttagaaaat agttgttcta atgatttttt
    31621 tctaaatatg cacaatttca aaggtgtaga atcgattgta gaagataaaa aggttaatac
    31681 tgctactagt gttctccact tcagcaaaat cttaattgat aataagttat ctaaaaagac
    31741 aggttttaaa gttggtagtg aagtatatta ccttcacaga ctaagatata ttgataacat
    31801 acctaaaatt ctcgacatta actattttct atgtagtata gttaaagacc ttgatgtttc
    31861 tatagcacaa ggctctattt ataaatatat agaagagtgt ataggtacaa aaattgtttc
    31921 atccagaaag atatttaaaa ttgaaaaagc tacagagctt gagttaaaaa cgctacctct
    31981 aaatgattat aattgtgttg gtgttattaa aaatagtgta tatactgatg atggaaagct
    32041 ctttgaatat acagaatcaa agcatacacc tgaaacattt gtattcatgg atgttactca
    32101 aagatattaa aaaaattatg tttaattaaa cataattttt ttacgtttag acactttatc
    32161 ccatactaac ctcactattc tatatacaat tatatgttta tacaaataat tatttattgt
    32221 atattttagc tttaatatta gtttaatact tgacaatttg tttaaacata ttataattaa
    32281 cttataaatt acaggaggta tcaattatga aaaattggtg gaaaaaagca actgtctatc
    32341 aaatatatcc aaaaagtttc aaagatagca acaatgatgg tataggtgat ataaatggta
    32401 ttatagaaaa attagattat ctgtattctt taggtgttga tttattatgg ttgactccta
    32461 tgtacgtttc tcctcaaaga gataatggat atgatataga agattattat aatatagacc
    32521 caaaatatgg gacaatgagt gactttgaaa aactcttaaa agaagctcat aaaagagaca
    32581 taaaaataat gatggatatg gtcctaaacc atacatctac tgaacataaa tggtttaaag
    32641 aatctaaaaa aagcaaagac aatccatatc gtgattatta tttctggaaa gatgctaaac
    32701 ctgatggttc tgttcctaat aactggatat ccagatttag tggaactgct tggaaatatg
    32761 atgaaactac taaccaatat tatttacatt tatttgaaga aactcaagct gatttaaatt
    32821 tggaaaatga aaaggttcgt gaagaatgtt acaaagtact agaattttgg gctgataaag
    32881 gtattgatgg ttttcgttta gatgtagtca acctactctc caaaacacct ggattacctg
    32941 atgaccctat aacagggcca aaaggagatg gaagaactca ttacgctgat ggtcctcgta
    33001 tacacgaata tttacataat atgaatcaaa aagttttcaa acctaaaaat atagttactg
    33061 taggtgaaat gtcatctact acacctgaag agtgtatcaa ctatactaga gaaaatcgag
    33121 aagaactatc tatggttttt agcttccatc atatgaaaac agattataaa ggaggcgcaa
    33181 aatggactaa tgaaatgttc tctctggata aattaaaaaa agcacaatct gactttcaat
    33241 ataaaatgta tgaaggaaag ggatggaatg ctctatttta ctctaatcac gaccaaccta
    33301 gagctttatc tcgatttgga gatgatagat tttttcgtca agaaagtgct aagatgttag
    33361 ccataacact atttggtcta caaggtacta catacatata tcaaggtgaa gaaataggta
    33421 tgacaaatgc ctactttact aaaatagatg aatatgatga tgctgaaagt aagaatgcat
    33481 actatcatat gattaaatct ggtattgatg aaaaggaggc attgcttata cttcaacaaa
    33541 aatccagaga taatgcccgt actcctatgc aatgggataa tactatttat aaggggtttt
    33601 ctaatcataa accgtggtta aaagttaata atgacaatat tagtgtagaa aatgagctta
    33661 atgattctcg ttcagtattt tatacttatc aaagattaat caaatatcgt aaacaatatg
    33721 atatttttac agaaggtact tatagattgt tagatgaaaa tcataaaaat ctatttgttt
    33781 atgaaagaga gtataaaaac caaaatcttc ttgttataag taattttact catgataatg
    33841 tagtatataa attacctgaa acttatttga ataaattaaa ctacacaata cttgaaacca
    33901 attacactag agatataatt gaaaataaaa tggaaataaa accttacgaa agtataatga
    33961 tatattataa ataaagttat ttatataaaa attggtataa caccagggtg ttataccaat
    34021 tttactaaat tcaaaataac atttatagaa tatctataga ttacaataat tatttgactt
    34081 ctacatcatt atatttcttt gaatatactt tagtcatata gaaatatata ggaactacaa
    34141 ttatcatccc acctaatccc caaactatct tcataggttc atcaatacct gcttttatca
    34201 gtaataatat acttataata actgcaaata ttggtataat tgctcctcca ggtattctaa
    34261 aatttacatc tttatcagca tacttctttc tcaatattag tacagctaaa catgtaggaa
    34321 tatattgaat aaatctagat acaacactta tttgagataa tgttgtgaat gaacccgacc
    34381 atgctattag taaagttcct atgacagata ctataattgc tatataagga gctccatttt
    34441 tattagtttt tcttatagac ttaggcatta atccttgttc aaccaaagca gcagcagacc
    34501 ttggtgtaaa tatggatgat gcaatattta tacctccaac agatactata gttcctattg
    34561 taattatata cccacctata ggacctagca tcttaatggc agcatctgca acaggagtag
    34621 ctgaccctgc caagccatta cccaacaccc ctatacttat tcccagtata agcatatata
    34681 ttactgatac tacaaacatt accattacca atgctttagg tacatttttc ttagggttct
    34741 ccatatcttt tgctgcaaca gctaaagatt caaatcctgt aaaagcataa aataaagtta
    34801 tagatactgc tacaaaagct gatgacgcac tttgaccctt ttgtattatt acaaatgggg
    34861 taaaattaga accctttata aagaataatc ctacagctat aaataaaact attggtaata
    34921 attttcctat agtcatgata ttatttaagt gcttagattg tttcattccc ataaaattta
    34981 taatcccaag tcctacaact aatataccaa ttattatatt tttaataagt ggttctgctg
    35041 cttgtggcca tacagatgat agtgttacag caaaaaaatt tgccatagta gcccaagcaa
    35101 tcatactaac tatccacttc ataaatccaa cttcaaatcc aacaaaatct ccaaatgcct
    35161 cttttgcata aagataaggc cctccatcag tatcaaacca actacctgct tcagcaaaac
    35221 ataaagcaat agaaacagct aaaagcatac aaaaaactaa tactaatata cttgctggac
    35281 ctatatctgt ataagcatca ccaggtaacc cgaatatacc tgaccctatt attgcattga
    35341 ttcctaatag tacaatactc caaaaaccta acttaatgcc tttttccatt gttattcccc
    35401 ccaaagccaa cttaatatat ttatattttt tatttagatg cttctttaac tattgccata
    35461 aatatttttt tcatattatc ataatctcta gtcatcattt ctggatgcca ttgtatccct
    35521 attacaaact cacttccttc cttttctata gcttcaatta aaccgtcttt agatgtagct
    35581 gaaacaatat atcctggtgc tactttgttg acaactaagt gatgaaaact attaactaaa
    35641 acttcttttt ctcctaatat ttcatataat tttgtcccct cctttatctg tactgtatgt
    35701 gtaggtacat tagatagatg ttgctgattg tgtttaatat aagcaccttc aattaaagat
    35761 aaatcttgat acaaactacc tccttcagtt acatttataa tttgttctcc tctacatata
    35821 cctaaaactg gttttttcat atctaaagca tgttttaata atttcatatc aaaactatct
    35881 ctctttggtg atattgcccc caacttatta tgaggttctt ctttccatat gagtggattt
    35941 acatcttgtc ccccagatag tataagcgca tctatattag atacctgctc ctttataatc
    36001 tcatcatcat atacaatcgg tacaatatat ggtatagctt tacacataac tactgattgt
    36061 atataatcat tatttacata agccctttca tagccaggaa acattcctcc ttcatcaatt
    36121 agtaaattac ctgatattcc tattattgga tatttctttt taaccaattt cttcacccct
    36181 taacttattt gtgaacaata aaaaatgcta cttctcttaa agagcaagta gcataccaag
    36241 attttattta tttgaacatt ataattcatt ttagaaatac tttttttgaa tacaagtaat
    36301 ataaataact ttatttactt atattttatt ttaatcctgt ttagtttgac ttttttaaat
    36361 tgttttaagt acaactaata ggtaaaatag gcatttagat tttccttttt atcaacctat
    36421 taaatactgt ttaatatatt ttttcccctt ctatttaatt cacttccctt tctaccagat
    36481 tcacatatta tcaaatccag ctcttttaac atattaagta ttccccttac ctcaccttct
    36541 gttatactgt agcctaattc atttattgaa ttaacaatac catttctacc tatattttta
    36601 ttaagattat tggcattttt taaacactcc attactaact ttactttctc tatagatgtt
    36661 ctatccttta atatgcttat ttctttacta aaatcttcag ttatatttaa taagttatga
    36721 ggtaaatcat ttattaaaat aacatcctca gaactcataa tgttaatata catagcagta
    36781 ttttttaatt ctcttatatt acctggccat tcatatgcat taataatgtc ttgaacacct
    36841 tgactaattg ctctcttcct tttcataaaa tgctttaaca taacttctat atcttctttt
    36901 cgctctctta aaggtggtat atttatgggc aatacattta tcctatagta taaatctgct
    36961 ctaaatctat ctccttttat catttctaat aaatttttat ttgtagccgc aattactcta
    37021 acatctatat ctattacatt ttctcctcca accctcatta cttgattctc ttgtaatact
    37081 ctaagtaatt tgctttgtaa gtatataggc atgtctccta tttcatccaa aaatatagtt
    37141 ccattgttag cctgttcaaa taatcctttt ttacctcctt ttaaagctcc tgtaaaagca
    37201 ccttcttcat atccaaaaag ttcactttca agtaaatttt ctggcatagc tgcacagttt
    37261 atcgctataa atgactgact atgtcttggt gatgcattat gaatagactg agccataagt
    37321 tcttttcctg ttccactttc tcccgttata agaattgtat aatcagattc agctattttc
    37381 atacctaatt tcttagttct ttccatcttc gttgaaactg tttttatatc atcaaaagta
    37441 tatctagcaa cttgtccctt ttctctaagc ttctttgata ggttttgctc caattttctt
    37501 atataagtta tttcttgaat acaaaaataa tacccagtaa cccttccaaa actctctata
    37561 ttatacttat ttacgtttaa gtacttatta ttaaatttta taacttcatc atgtgcctta
    37621 ctcaaactaa aaatcttttt aatattctca ttctctaatt catctatatt tttccctata
    37681 atattacttt gaatatctag tatatccctt aaactattat tgcaaataat tatgtttccc
    37741 ttatctgata ccattgccat acctaagttt gataaattta ttatagaatt taattcatct
    37801 gcttttatag taagttcttt gtatttagta tttattcctg aatataaact aattatttca
    37861 tctgtatact taattagcct ctcagtaatt tctttatcat ccagtttgag ttttgctatt
    37921 atttgaataa acgttgaact atcaatatac ctatggccta aatcaataac ttcttctata
    37981 tattttggaa ctaaacttga ctctcctggc gttatagcta tcttaatatt tctatattct
    38041 tcattgggat tgtatggtat aaaattcaaa tggtttattc ctatattgta taaattagat
    38101 attgtttcca atacagtatg cttattatcg tttactacta atacatctat ttcttctggc
    38161 aatgaaaata acttatatat atccttttgc tttatgctcc tatttatagt tattatcttt
    38221 gagacatctt ctacatattt ttttattttt aatactctat catctatcat taccaatatt
    38281 atgtcatctt ttattaattc cttctctgtt attttattta aataataatt ttttatcatt
    38341 actttatctt taaatatcat ttctagattt ttatttaaga aacattctaa tttagacaac
    38401 ccatcagtta ctactgctat tgttttcatt tgattactcc ttttaaaata tactctactc
    38461 ttttataata tacatctgat tttcacttta atagcacaaa aaattgtaca tttagtatac
    38521 taacatattc ctttattagt tttatgtccc taaatatcat agttgcaata tctatcatat
    38581 ttcttaaact tctataaagc aaaaattgtg ccaaattaat aattttctta attttattct
    38641 aaagttcctc aatagtattt acagacttag cacttatagc ttataagtct ttatattatt
    38701 aatctatttt tataggttaa ttgtacaaat catatatcac ttttacctat taaatgcatt
    38761 ataaaaaaga tggaataacg attcgtcact ccaccttttt tgtaataaaa aattgtatat
    38821 ataaacacta tttactactc ttctaagtac aaacagtatc tacatactat aacctttcac
    38881 cattagattc tattacttgt ttataccagt caaaactctt tttcttaatc ctagacaagt
    38941 ctccacttcc atcatcatac ttatccacgt atataaaccc atatctcttg gtcatctcac
    39001 cagtagatgc acttactaaa tctatacatc cccatggagt atatccaaat aaatcaacac
    39061 catcttctac agcttccttc atttgctcta tatgtgcttt taaatactct atacgatagt
    39121 catcatttat cactccatta gattcttttt tatcataagc tcctaaacca ttttcaacta
    39181 ccatcatagg tattctatat ctatcataaa tttcatttag tgcatatcta agcccttcag
    39241 ggtctatttg ccatccccat tcactagact taagatatgg atttttcact ccacctaata
    39301 tgttgccact tgtattatct tctttagaat cactacttac acatgtagac atataataac
    39361 taaatgtata aaaatctaca caaccttctt ttaaaatttc taaatctcct ttttccattt
    39421 ttatttcaat attattttct ccaaaatatc tattcatata agttggatat tcgccacgca
    39481 cttgtacatc tccacagaaa taattcatta ctctactatc tctttgagtt ttaactgcat
    39541 catctggatt acaagtataa ggatacattg ttgcaaatat ttgcatacat ccaacttgat
    39601 aatttgaatc tatttcatga gctaatttaa cagccttagc acttgctaca aattgatgat
    39661 gtaacccttg aaaacgaagt tgaggtatat ctacttggtt tataaaatct gtagtccctt
    39721 catttaatat tccttgtgat aagtagcctc ccataggcat tgtagctgaa tttatttcat
    39781 taaatgtcag ccaatatttt acttttcctt tatatctttt aaatattgtt tcacagtaat
    39841 tcatatacaa gtctattacc tctcttgaaa cccatccatt gtatttctct gttattgcaa
    39901 atggtatttc atagtgagat attgttatta atggttctat attatatttt aaacattcat
    39961 ctatcacatt ttcataaaat ttaagtcctg cttcatttgg ttctttctcc attccagttg
    40021 gaaatattct agtccatgct attgaaaatc taaatacctt aaatcccatt tctgcaaata
    40081 aagctatatc ttctttgtat ctgtgataaa aatctattgc ttcatgactt ggatagaatg
    40141 ttcctttttc tataactctc gttattcttt tacttacttt ttgagaaccc ccagtacaca
    40201 tatctgaaca agaaggacct ttcccatcta aattccatgc tccttcacat tgatttgctg
    40261 ctgttgctcc accccataaa aaatcttttc taagtaccat tactctacct ccaataattt
    40321 atttatagta aatttatttt aaaataaaaa tacatatatc taatttttaa ttaataaaac
    40381 tctcttttaa gctaaaacag ttatcaattc ctgactatta tttacatgct tttgtgtatt
    40441 ttctataata tccacataat cagaataatt agtaacaact actggtgttt ctaagcaata
    40501 tccttctttt gatatgcttt ctatatcaaa agttattaat gtctgacctt ttttaatctt
    40561 atccccttga ttaacaaaag atttgaaatg tttaccttcc aactgaatag tatttaaccc
    40621 tatatgtatc aatagttcac aaccattatc agatataatt cctattgcat gttttgttgg
    40681 aaataaagtc attatagtcc catcaaatgg tgccactact tttccttctt taggtgttat
    40741 tgctacacct tttcccaata tacctgttga aaacgctgca tcttgtatct gctccaaagc
    40801 ctttacttca ccttttattg ggctatatac tacttctctt ttgttttttt ctacaacgcc
    40861 ttgcccatta ttatctttag aaatttctac tatagaatca tctttccaaa aaaataatgt
    40921 taaaacaaat cctactacaa tagctactat tattgctatt atagttgcat aaacaccttt
    40981 catatctcca gttgttggat ttatataatt aggtatacca aatattccaa gtccacccat
    41041 tacataactt tttacattca ttatccccat tacagctcct gccacagcac cacctacaca
    41101 actatatatg aatggtgttt ttcttggtaa agttattcca tatattgcag gctctattac
    41161 accacatatc cctgaaataa ctgcaggtat acacagctct tttatcttct tatcttttaa
    41221 tttaaagtac attgctgcta ctactgatgt ttgtgcaaaa gatggtacaa atgctcccac
    41281 taatattgtg tcaaacccca tagtcataag gtttgacata gctattggta ctaatcccca
    41341 atgtagacca aatattacaa gcacttgcca caataaacca gttaatacac cacataaaat
    41401 tggtgaaaag ttaaataaag ctaaaaatcc atttccaact atattagaag cataggtagc
    41461 tataggccct ataaccataa atcccaatgg taaagaaact aataaaactg ccattggtac
    41521 aaagaaattc tttactacat ctggtattat tttagcaaac actttctcca acttagctgc
    41581 taataatact attataatta ctggtactac tgttgaagtg tagttcattg ctattactgg
    41641 taatcctaag aaagttatat atactggtga ttcaaatatt gttccagtaa atagattgta
    41701 taaaggttga cctatagatg ctagagtatc taattgaata cctgaatagc ataaaactgc
    41761 acctataagc attccaataa atggttttaa tttgaatttc ttagcagctg tatatccaag
    41821 aattattggc atatactgga acattgcatc tccgattgca tttaacataa tatatgcacc
    41881 atcagtttct ccatacaaac ccaatgctgt aaatactgca ttaaatcctt ttatcatacc
    41941 agcagcacac agaactccta atattggttg aaaaactcct gatattatgt ctataaactt
    42001 attaagtaat ccttttggcg catctgaatc tgaattatct attgtatctc ctcctaatcc
    42061 agccaaatcg cacacaactt cataaacatc tggaacatga ttaccaataa ctacttggta
    42121 ttgacctgca cttttcatta cagtaactat accatccatt ttttttagca catcatcatt
    42181 tgctattgat tcatctttta atttaaaacg tagcctcgtt atacagtgag ataaactgct
    42241 aatattatcc ttccctccta cattctcaat gatttctttt gatagttgtt catatcttct
    42301 cattttttct cctccatttt ctttttgaaa tatgaaggtt tggccaactt aatgtcacca
    42361 cccaatatat ataaacctaa ttattaggat ttatatctga cttaatactt ttacttacca
    42421 ctctttcaac gtgaatagtt aagtataatt tttcctcatc tgttaaatcg tagttgtatt
    42481 ttttatgtat aaattcatcc aacttttcta tgcatttata agcattttta tatttaactt
    42541 ttattacatt tagtaaatca tcttcattat tttcatgtaa attattacta acaattcgtt
    42601 ctgcaaaata ctttaagtga gtaataaatc tatgataata aactgaatct tcatcaaata
    42661 ctattctgta atggtattta accatatttg aaatttcttg tattatttgt gtaatctcat
    42721 atatagtttt tatactttga tccatttgtg cattaactat atgcagtgct ataaatccag
    42781 cttcatcttc tgatagtcta atttcaaatt ttttttctat ataatccaat gcttctaacc
    42841 ctattttaaa ttcactctta taaaatcttt ttatatccca aagtaataca ttttttacac
    42901 ttactccttc acgagctctt tctattgcag tatacatatg gtctgttaaa gaaatataaa
    42961 tactatcatt taatcttttc cctagttttt ctttagcata taaaattatt tcatctgata
    43021 attccatata tcttatagga atatctgcta ttagttcttt aaatttatca gatatatttg
    43081 ggtctgaaag tttgaatatc ttatcaattt tatcctttga tatatgctct cctgtacgtt
    43141 ttttataagc taaacctctc cccataacta ctatttcctt atgattttca tcagttgtaa
    43201 taattacatt attattgagt attttttcaa taatcattgt gatgccccct ttattagcat
    43261 taagttaaaa taaatctttt ttaattattt agaatattta aaatgtaatt tattacataa
    43321 aaaaaaacct atttattaca cattaaatag actcccatga gtcctgagta ataaataggt
    43381 ttagcttgta tttaaacaat cactatccgt ttgtatttta ttttaattat tatttattat
    43441 atgttagcaa atttattttt tagtgtcaac tttttctcaa tattttaatt ctaaataaat
    43501 tatataaatt gcatctgcaa agtactattt ttataaataa acaaaataaa aaaggtatta
    43561 cttgctttaa gtaataccca tttattttta atttaagtgg tttttcttaa tctcatcaag
    43621 tagtccatct tctaaataaa tttctaatgc acatcctacc attgccttta ctgctttgtg
    43681 aagtttatca taagcttcct cagaatttgc ataatttaga aattcttttt catgaacata
    43741 taccattgga cttatatcta aggctatttc tgtatacata gtagggcaaa cttgacttac
    43801 attacctata tctgtagaac ctactggacc atctttacct tctaatatat ctgttatacc
    43861 tgactcaatc aaattattct ttgttatctt ttgaagtact ttgttattta ttatgttgtc
    43921 aaaagaattt tcaaagtatc tataagaaag cttagctcct gtcattaact ctgcaccttt
    43981 tgcacagttt ataacttttt tagtcacttc atctaaataa cttctttcag cagctcttac
    44041 aaaaaactta caagctgctt tttctggaac tatatttgga gcctctcctc catttgaaac
    44101 tattccatgt attctaacat ctgattttac atgttgtctt agtgcattta tcccagcaaa
    44161 agtaagttgt actgcatcta gtgcattaac tcccatatga gggtgactag ctgcatgtgc
    44221 tgatacccct tcaaaactaa actctaatga atctattgcc aaagccttac agtttatatt
    44281 attaaaagct tctatatgca tttgataact tacatcaaca tcatcaaaag ctcctgcctt
    44341 aactaaatca cattttcctc ctgttgtttc ttcagctggt gttcctatta gtacaatttt
    44401 tcctttaaaa ttttctttta acttacttaa tactatacaa gctcctagtg tagatgctgc
    44461 aatccagtta tgcccacaag catgagctgg ttctttattg tctccatatc caggaagtgc
    44521 atcatactca gctaaaaatg ctatggtagg tccatcatta tctcctaatt cagctctaaa
    44581 agcagtctcc atattacaat atggataaac tgtatcaaat ccatattcct tcattttctc
    44641 tactaagtat tttgatgata tgtattcttc ttcccctaat tctggattgt taaatataaa
    44701 attacaaact tcttcaattt cttttccaag ttcattctct atctcataag ctctcttctt
    44761 tatactctct atcataactt acctcccact agaatcctag atataatcct attgtcaaaa
    44821 ataccattac aagaattgtt aatactacca ttaaaggaat tacgaatcta acccactttg
    44881 accaaggaat attggctact gctagtgctc ccattaaaac acctgatgta ggtgttatta
    44941 agttagttac acctatacca aattgataag ctgtaactat catttgtctt gatacatcta
    45001 ctaagtcgcc aagtggtgca agtacaggta ttgtaagtga tgcaagccct gatgctgatg
    45061 gtattaaaaa tgctattaat ccttctatta agaaagttaa atttataaat atagtttttg
    45121 gtaatccatt taataaattt gctgcagaat ttaaaattgt atctattata aatccatttt
    45181 ctgctattat tacgattcca cgtgcaaatg ctatacataa ccctgctgta attaaatctc
    45241 ctgctccact tataaatgaa ttaactatct catcttgttt aagccctgat gctattcctg
    45301 atattatacc tatagctgta aatatcattg caatttcagt tatgtaccac tctagtctta
    45361 aaactccata tatcataatc gccataccta ttgtgaaaat ggctattacc atggcttctc
    45421 tttttgtaaa ttcctttata tttgtatcac tattaaccat tgattcttga tttagtgata
    45481 tatcgtgtac taaagatttt ttagggtctt ttttaacttt attggcatac atcataacaa
    45541 atcctataga tatagccata tatattataa acataacaac tctaaactct attccagaac
    45601 ctggtactat ttgtgataat gcttgtgcta ttcctacaga gaaaggattc gtagtagctg
    45661 ctccaaatcc agctgttgct cctataaata ctgtggccac tgctgtcaat gaatcatatc
    45721 ccatactggt cataagtgga ataaatatca tataaaaagg tagtgtctct tcactcattc
    45781 caaacgtcgt cccacctagt ccaaatagaa tcattgaaat cggtattatt aaaaattgaa
    45841 acttcttcat tttatttaca gtatgagcta taacagcttc tatagcccct gttttattaa
    45901 ctatcccaaa agctcctcca actattaaaa caaatccaac tacttcagct gcatctataa
    45961 atccgtttat aggtgctgta aatacatcag taattccttg tggatttgat ggtgcttttt
    46021 cataagttcc agctacaaca actgaccttc cgtctatatc tactctttca aaatttccac
    46081 ttggcactat ccaagtaaga attgccatta acactattag cgaaaatatt attgtaagtg
    46141 tctgtggcat agtaaacttt ttctttttta aactgctcat ttttaatctc cccacttttt
    46201 aaattaaata ttttattatt gcatcccttt atcctatttt gtatagatgt aaaaaagctc
    46261 aacatggata catgttgagc taaatgtgct aaatacaaca tacctgcatc ttccctttat
    46321 cttgtttaag atagctctcc atcaaaattc gggataattt tgatgacagt actgtaccta
    46381 ttaaatacag tcccagcact agctttggac actagcactt cggcacttac tccttttata
    46441 ttgcctcatt ggtttccaac ctcttcaata ttaatcttag taaatgcaac ctctacctca
    46501 atctgttcgt tagatgaggt aattcttatt aatttttatt gattcaaagt aattgtagca
    46561 taatatgtcg agattgtaaa tattttcagt ataataattc ttactttatg atgtcattcc
    46621 atttttcaca aataattttt tctccatata atttaaatac tgtagtatat tttaaataca
    46681 cacaaacttt ccacaaattt ttaattatta taataccttt ataataaaaa ataaaagcca
    46741 tgtaaactta tcaatttaca cagctttata tacattaatc tactccaagt acagctttaa
    46801 gctttgatgc tattgtaata actcgtggac caaatataac ttgaactgag tttttctttt
    46861 taacaactcc acttgcaccc aattcattta cccagaaacc atcttcatag aatccatcaa
    46921 ttgaatttag ctctacccta agtctagttg cacagtttgt aacatttttt atattattac
    46981 ttcctcctaa accttctatt atagaatgta caagctttgt atctttatca tctacttcct
    47041 cttctactgc attgttgtat gctgttttac cagcatctac agattgtctt tctctatatt
    47101 ctttcttact ataaagctta acttcttctg aacttcttcc tggtgttttt atatcaaact
    47161 tcttaattgc ccatgtaaat attatataag taactataaa aaatattggt atcaaaaata
    47221 ataatggtaa cgcatccact ttttgtggtt gtaataaatt aggaaacata tttctaaatg
    47281 attctcccat tatagaaaca ttaaacattt ctgtaaatac atacgtcaat cctgccagtg
    47341 gtacatatgc tacaaaatac aataatggtg caacaaatag gaatgtatac tctattggtt
    47401 ccgttacccc taccaacatg gctgttacta ttgctggtat caatatactt attgcttttt
    47461 tcttattagc attatcagcc aacttataca tagctaaagc tgctgctggt aatattgcaa
    47521 aatgtattaa taaacgacct gttgtgaagt tacgagttat ataagatagt gtatctgggt
    47581 ctcccatttg agctaatctt atattattaa caccctcgta aattgctcca cctacttcca
    47641 ttgttccacc tactcttgta tattctatag ggaatgcaat taagtgatgt ataccaaatg
    47701 gtaataatgc tttatccatt gctccaaaag taaatgttcc taataatcca ctagaaccta
    47761 tccaattacc aagtttagat aatgccaacc ctactggtgg ccatataaag tacattgctg
    47821 tacccaatac tattgatgct ataaacatca taatcattga aaatcttgga cctgagaaaa
    47881 aagataatgc actaggaagt tctatgtcaa caaatttgtt atgtactgca ctagctacaa
    47941 atccagctat tatagctcca aatatactac aatcatatga aaagattcct aagaaagaac
    48001 catataaaga acttcgacta tatgcttctg cctctcctaa tccagatgcc ataaacgctt
    48061 ctgctgtaac tgtatctgaa tttataccat ttgatgctaa caatgtactt ataactgtgt
    48121 gcattcctat aaataaaaca attcctccta atgcacccca tcctttttcc tttttagata
    48181 atccaaaagc tatacctgta acaaagaata ttggtaagta tctcattacc ataaatccta
    48241 aatcgtttat tactttaaag aatacattta taaatgaccc ttcttttatt agtccgctaa
    48301 gccctaatgc agaaacgttt tccatatttg caaaagctga ccctaatcct ataaatactc
    48361 ctgctacaac aagtaatata acaggtatca tcattgctcc agaaagtact tgtaatttat
    48421 ccttcatagt attatttcct ccatttttat tgaaatgcca ggctatataa tgcatatata
    48481 tatacttcta ttgctttgta taatgtatct atacttatat tttcatttgc ctgatgtgaa
    48541 tttggattat ctccctctat tgtgggtcca aatgcaactc cattttttaa cactcttgca
    48601 taagatgcag ctccttttgt aaaaagttcg gcttcttttc caaaaccttg tttatatgca
    48661 cttgataatt tatttattaa ttcactatct ggttttacat acagtaattt taaatcctta
    48721 tatatatcaa ctattaaatt ttcttttttc ccaatttcat taattctatt agttataaac
    48781 tcccaactta tacccttagg atatctaaag tctattttca tattcaattc atttttctct
    48841 aagaagatac tcattataca tattgtagaa actcccatat ctacatcttc tttatatatc
    48901 cctaattttt ttccatgatt atcatctgta aagtatgaat ttaaaatatc ccttattata
    48961 acgcctttat catttagctt tttaattttc tctaaatctt ttgctaaact aaatgcacag
    49021 ttatatgacc tgtgtggatt tctcgataaa gccctttctc ctgtatatct tactaatact
    49081 ttaccttctt ctagttcttc tatctctgta taataagcta atgattctac taaatctttc
    49141 ttatcatcag ttttaaaaac agcctcaatt ttgtcacata caaatccatc ttctttgttt
    49201 gactttatat caactaaatt gtgatttcta aatttatctt tatctatttt ctttcttaga
    49261 ttgaagtata aaataccatt ttctccattt actattggga agtttccatc tggagaaaat
    49321 gcaaattttg gctgttcatt gacactaaag taatgctcca tacattccca agtcgtctct
    49381 tcagctccac ccactatcaa tctaatcttt ctcttcggct tttcattaag ttctcttaag
    49441 aaaagtaatg catataaaat ccctatcaaa ggtcctttgt tatcattaac tcctctacca
    49501 taaagatagt tatctttttg acatacttta aaaggtttac tcttccaaag ttctttatta
    49561 tatattggca ccacatctat atgattaagc acccctacta cttcttctcc ttctccatat
    49621 tctatatgaa ttgcatatcc atcaaagtct tttacaacaa aatctttgtc ttttgctatt
    49681 tgaataagct tatcgaaggc atttcttatt tctatcccaa atggttgatt tatatctgtt
    49741 gtactttcat ctctaattga aggtatttca actaaacttc taatatcctt tatgatattt
    49801 tcttgattgg attttaaata atcaataatt agttctttgt ttactatcat cttaattatt
    49861 tatcacccct ttatattaat tttacaaagc atttcagttc atatctacat tataaatttt
    49921 gaatattatt ttttcaatat ttcaaatact tctctaaatt accataatga tacggtaaac
    49981 aatcaacaga ttatgacaaa atcatactat acctattact aatattttaa cttatctatt
    50041 acgtcttttt taatgtaatt ttattccaaa aatagatata aaaagatgta gccttagcta
    50101 catcttttta tatctattca acttccatta ttatgctatt aatatctaca tttccaggtt
    50161 ctatcctatc ttttactgtt ctcttaacag aatcagcatc atccattata actacaggag
    50221 ttattatatt taaatcattt gacgttataa aatctaaatc tacttccatt attaaatctt
    50281 tagcttttac cttatctcct gcctttacct taacatcaaa cggctttcct tctagtgaca
    50341 ttgtctctaa acctacatgt attaatatat ttgtattatc attactctta atcattattg
    50401 catgctttgt atcaaataca ttgactattt caccatcaac aggagaatat aacttacctt
    50461 catttggtat tattgctact ccatctccca tcatcttttg ggcaaaaact gcatctggaa
    50521 catcttctaa gttcactaaa tttcccttaa aagggctata tattttctca atcttatttt
    50581 cttttttctt aaatatatta aacataacac actctccata ttataaaatt ttatgataat
    50641 atttttctta aagcttttgc tacaccatca tttacattag tatcagttac aaaatctgcc
    50701 atagatttta cttcatctgt agcatttccc atagcaatac caagacctgc aaatttaatc
    50761 atggatatat cattttcatt atcacctata caaataactt ctttgctatc tatacctaat
    50821 atatcacaaa attttttaac tgcaatacct tttgaagtac ctttactcat aacttcaaaa
    50881 tttatctttc cagaacttac tacttctaaa tcttcttgtt ttttaaattc ttcctttgct
    50941 tctctcaaag aatctacgtt ttctgagaaa agtatacatt ttaaaatttc attatcagct
    51001 ttttctaata aagtttcaca acttgcattt tctattattt caattctgtc ttcttcttta
    51061 tttttactat ttattaatct atatggatgt tcttcaccta tttcattgtt ggatataact
    51121 attttacagc ctttaaaatg tttgtttaaa ttatactttt cacctattgt atagattttc
    51181 tttatagctt ctttgctcaa aaccttctta tactcatatc ccattaattt aaaatatgtc
    51241 ccattagttg atataatata tatatcatcc cctaaaacac ttgtaaaata tttcatagca
    51301 ttataaggtc ttcccgtaac taaggctacc ttgataccct tttccatagc ttcttttagt
    51361 gctttaatat tttcttctga tacttcaaat cctttcccca tcaaagtacc atccatatca
    51421 gtacaaatta atttatatcg catttgtatc acatcctagt aagatttttc taatatttca
    51481 acaaggtctt ctttctttcc aaaaggtaaa aatcctaaaa ctggacagct tgttatttgt
    51541 tctaattgtt cctttgttac attaaactca gacatttttg tcttaagtcc cactctcttt
    51601 aaaaaacttt ctatttcatt tcctaaagtt ttagctgctt catcatcttc cactttttct
    51661 aattcttggt ttagcattct cgctactttg gcaaatttct ctatattatc cttatagaac
    51721 ttttctgcaa attttgggaa cactatagcc aaagcctcac catgaggaat atgtgtaact
    51781 cctcctatta tttcacttag tggatgtggt gctgctgctc cagcatttgc cagtgaactt
    51841 cctcctaatg tatctgctac tgctaacttt gttctgtatt ctatattatt tttatcttcc
    51901 attactttag gtagatagtt aatgactaat tccatagatt tttcagacat tatctctgta
    51961 aagtcattag catttggatt tatataactt tcaaatgcat gagtaaaagc atcaaatgct
    52021 gttgatgcag taattctctc tggcagagtt aacataagct ctgggtcaac tatacattct
    52081 tttgagaaac aattttgatg aaatattgta tttttctcgc ttcctctcga tattactgct
    52141 gcttgtgtaa cttggcttcc tgttccagaa gtagttgata cagctattaa tggtaattct
    52201 ttttcactta ccttatcgta tattttaaat ggatttgtat atgtactaaa catataatcc
    52261 caatctattt tttctaaacc aaaagtcaat gctaatactt ttgcagtatc tatacttgaa
    52321 cctcctccaa cagctaaaac aacatctaca gattcttttt tagctaattc gaacccttca
    52381 tttattaatt ctacagttgg gtttggctct actctatcaa agtgtacaac ttctatacct
    52441 ttttctatta aattcaatgt aactctttta tataacttgt ctaatggctc tacatttgga
    52501 gtagtaacta acaaacattt ctttccatat tttcctatta ttctaggtag ttcacttaaa
    52561 gttccttgtc caaaatttat tcttgatggt tgataaaagt taatcatatt tttcctccct
    52621 taaattattg acttcatatt caaattatac atatcatagg atttttaagg caataccttc
    52681 actgttaact cttatttact aaagcttatg gcaattatta gacaataaat gttttattat
    52741 taccatatca ttatgtaaat tttattaagt acccatgtta taattttata atcaatctta
    52801 ttgtataatt gacttaataa taaaaataga gtatatacac ttttttatga caaatagctt
    52861 aaaggagaat taaattatga aatatttgat agacagtgct aatcttgatg aaattcaaaa
    52921 gtgtttaaag tatggattta agggaataac agctaatcca tctatgtatc ttaaagagaa
    52981 ggtaaacttc catgaattta ttgagacatg tactaattta aatccagaaa tattaactgc
    53041 tgaagtcatt ggaaataatt tatacgaatt aattaattca tgtgataaga tattaaatat
    53101 aaataaaaac actataataa aaataaattt ctctgaagtt ggcttagaac ttataaacat
    53161 attaaatagt aaaggtgtaa aaactgcatg taccttgatt ttcaatgtaa atcaagcatc
    53221 attagctata aatgcaggtg ctgattacct attccctttt ataggtagaa atgatgaaaa
    53281 tgggaatgat ggtataaaaa accttacaga aatatgtaac cttatatcaa taaatggata
    53341 taatactaaa gttgttgcag cttctattaa aaatgtattc cacttaactc aagcatctct
    53401 ttgtggatgt gattatgcag ctgtaacata tgatttattt aaaaaagctt cttttaacca
    53461 gcttacagta gatggtgcca atacttttga aagagattgg tccaaggtta ataaatagta
    53521 actaagattt aggagggttt gcattgaaca aaagattatt agatattgct gaattattac
    53581 tcaacagctc agactatatt actgtagata ctattgccga agaattaaaa gttagtaata
    53641 aaactattcg taatgacctt gttattttag atgaatggct tttagagttt aatttatccc
    53701 tagataaaaa aactggttca ggggttatta ttctaggtaa tgaagatata aaacttaaag
    53761 ttattagaga tatcaatgat aaatcaaatt gtatatatgc atactctcct gaagatagaa
    53821 aaaggtatat tctaagtaaa ttatttatta aaaattctaa gtttaggatt cgtgatattt
    53881 gcaatgaact acatgttagt agagctactg ttcataaaga cttggtagtt attcaaaatt
    53941 ttttgcaaaa ctttaaagtg actctagtta gaaaaaccaa taatggagta tatttagagg
    54001 gaaaagagaa agatattcga aaagcagttt ttgaacttgc tactgttaat aaaagttata
    54061 ctgaacttaa agaaatatta ttctcgaact ctaatgaatg caaagatacc ccatcaacaa
    54121 aaatatttaa ggagctattt aactatgatt ttgagaaatt atctcaaatc actctacaca
    54181 ccttacaagt agaaaaaagt aaccttagtg atgagtatta tataaacttc ttaatacata
    54241 tagctatatg tattaagaga atttcaatta aaagatatat taacttatct gaaggttttt
    54301 ttagagaatt attagaacat gaatactttg aaaaatcaaa aattttatgt gctaacctaa
    54361 gtgaagcttt tgacgtagaa tttattgatg aagaggtctg ttatatactt cttcatatag
    54421 aaggattact taaatcaata aagaaaacta ctactcttga acctcttaaa aatgaagaaa
    54481 ttacagagta taatctatct gaagcaatag ctgttcattg gggaagtata ctaaatttaa
    54541 acttaaagga tgatttaata ttaataaagt ctcttgctaa tcaccttaaa tcagttctac
    54601 acagaataaa ctatggattt actattacaa atcccatatt aaatgatata aagagaacat
    54661 ttccttatac ttataaggct gctaaagagt ctaatactgt tatcaaacag ttgatgaatt
    54721 atgaagtcaa tgatgacgaa ataggatatc ttgctttata tttaatatca gcaatagata
    54781 gaagcaaatc tcccctaaat actattttaa tatgtcactg tagtgatggt gtatctaatt
    54841 tattagtcca aaagttaagt tttgaattta atcagattaa tatagttaaa tctatacctc
    54901 tatcttctat ttcatttacg aactttgatg atgtggactt aatactaacc actgctcctg
    54961 tagactttga acataatgct gaactgataa atataaatgc tcttttaagt aagaatgata
    55021 tttccagact tagtacaatt ataaaaaaac tatattctga aaaaaataaa atattatctt
    55081 ataaataaac aaaaataggc ttgtctcaac atagagataa acctattttt cattgtccaa
    55141 atgataaaaa tatatttgat ttcatagaaa aagtgcctca tgaactaaaa aattcatttt
    55201 gagacacctt cattttttat atttaatctt tttatttaag cttttatatt ttttaaactt
    55261 ttatattttt taaactttta tatttaaagt aaattttatt atttttattg agcgcaagca
    55321 ttatttatat tattaatttc ttcagtattc ttaccaaact ttgtagttac aaatcctatt
    55381 gtttttatta tgagtaataa ggatatcaat gaatatatag atatcataaa attcttactt
    55441 attattccag atgctactat tataaatcca tctagcataa ataatataac tggcaacttc
    55501 agaaacttaa ttaccttatt aataagcatt gcaactaaat cagttccacc agtactacag
    55561 ccccttatta ttgctatccc tatagcagtt cctaccaata acccacctat cactcctgct
    55621 actaatagat tgtcagttat gtgagtttgt ggtattactt ttaagaataa tggacccata
    55681 agagttatat ataatgtttt tattccaaaa cctttcccta aaaataataa tcccattact
    55741 aaaagtggca tagatattcc taatgatata ttctcaacag gcattgataa gaatgtgcta
    55801 agtacgactg ataatcctgt tatccctcct ggagctaatg tatgttctgc aaagaaaaaa
    55861 ttaactgcta atgccattaa aaatgctgat attgttatca gtaaataatc ttttatcatt
    55921 tcttttttca tttaataacc tcctatgatt atattgtttc agtataaaaa atcaattaaa
    55981 ttagtctagc ttcctgtagt ttttcataaa attaatgctt ccttaaaaca ttaagaaagc
    56041 atctctttta tcatagttta ttatcttatt ctaaaccaaa ctttgtagct gcttcatcta
    56101 tagcttttct acaattctta acacctgctg ttataccatc tttatccttg aatattgctg
    56161 aagataatat tacaacgtct ggattacatt ctattaatgg ctccatattg tctgctctta
    56221 gtccaccatc tattgctaat tcacacttag gattcttttc gtctatcatt tttctagctc
    56281 tttttactaa atcaacagca ctctttctcc atccccagtt atcttttcca tcagtttcat
    56341 caacaccatg aacgactatt tgcaatctat ctatatcata tatagcttca tcaacaaaac
    56401 atagtggtgt gtaacatcca attgttaatc ctactttcat accaaattct ctacagtaat
    56461 ttattatgta tgcaagagga gcgcctaaaa aatgttctgc tggtattatc aacaagttac
    56521 atcctgctgc tgctattttt tctataaata atctatcaca atctcttgtg tatatatggc
    56581 attctattgg tttatctgtc actggtctta ttgcttctat tatttgatga ccacccataa
    56641 gttgcatatt ttttaagtca tgcatatctg ctgcatctga atgtacgtag tctacacctg
    56701 catccattgc ttctttaact acggctgata tatgaccgta atttacatgt gctaatcctg
    56761 ctgctatttt tatgtgtttc atatttgtat acctccatat tttactttaa ttagtattaa
    56821 atattagttt ttgatatttt ttaatccagt attactttta tttagcatta cttttattgc
    56881 tgtatatatt tcttttatga acttattctg cttttgttaa ctgtgaactt attttgcttt
    56941 gtaaattaat attacactga tatatttttt ttcacaacac tttgactact ccttaaaagt
    57001 acctttatat tgtggtaaat tatagacatt aattgtatat aaaaagttag ttttttacca
    57061 cattcttatg gcatttattg tctatactaa ctcaattgat atagctagtt catttaatat
    57121 ataatcagac tataaatata aatttactcg ataaaaagta ccattcataa caatattaat
    57181 gaaagggaga tgtaaaatga gaaactgtat tattgcccaa tctggaggcc ctacagctgt
    57241 tatcaatgct tctgcagttg gtatttatga aaaaaattta gaaactaaat actttgataa
    57301 agtatatttt ggtatcaatg ggattgaagg tatattaaac aaaaatataa tagattcaga
    57361 ctccttaaac aaagataatg tattaaattt aaagcaaaca ccttcgtctg gtcttggttc
    57421 ttgtagatac aaactggctg attataaaat aaattcttat gattatgata gagttataaa
    57481 tatactagat gaatatgaaa tagatacatt attttatata ggcggaaatg attctatgga
    57541 cacagtgatg aaattttctc aatactgcaa aaacactact tgtaaaataa aatttatagg
    57601 gattcctaaa acaatagata atgatttatt aggaactgac aactgtcctg gatttggttc
    57661 tgctgcaaag cttatttcta cactaataat ggaaacatac ttagattttt ctatatacaa
    57721 aaataatggt atatttatag tagaaaccat gggtagagat actggatggt tagcagcttc
    57781 ttcttcacta gctagactta atgatactcc acttgttgat ttaatatacc ttccagaaat
    57841 tgatttctat gaagataagt ttatagaaga tgttagaaaa atatttaaag aaaaaaataa
    57901 ggcttatata gttgcatcag aaggtataag ggataaatac gggaacttta tatgcgaaca
    57961 aaaaacttct gctcatgata aatttggtca ttcccaatta ggtggagtaa gcaactatct
    58021 aaagaatctt atagtatcat catcaattac aacaagagta aaaaccttag aacttggtgt
    58081 aatacaaaga tgtgctatgc atttagcttc agatactgat ttaaaacaag cagctgaagt
    58141 tggtgcatat gggatagaac tatctaaaaa aggatataca ggagttatgg ctatcattaa
    58201 tagaacatgc aataatcctt ataagtatga agttagtcat actgatattt ctaaaatagc
    58261 taacaagaca aaatattttc ctaaagagtg gataaatgaa gaatgtaatt atataactga
    58321 tgaagcccta aattatctat atccacttat tcaaggtaaa ccagatatca aatataaaaa
    58381 tggactacct tatttttcaa ttatttacta ggaaattatt ataatttgat atacactcaa
    58441 ttctatcgct cttaaatacc taagtggctg ttaaataaaa ttataaattg ttatatgaca
    58501 gtcacttaat cgtttaatta tttaactgtt tagtttttta attttttaat cttttttaat
    58561 actaatacga tttgttgtaa taatatctca cacccatttt cattttggat agattataat
    58621 tagttagaca ataaatattt tgtcatgata ttattatata tagtgataca aatagaaata
    58681 cttttatcat gactagaaaa acaaagatta cacaaaggat ttaaaaatca aacaacttca
    58741 attaaaataa atggttaaaa taacttctaa atcaactaca taaacaataa aagaacataa
    58801 agtagttaaa tctacaatta taaaattagt aagactataa taacttaaat aaaattaaag
    58861 ttaacaaaga ctattaaagt tagcaaatat tcctctacct aaataattgc tttttataaa
    58921 gagttattta ttttaattta ttttaattga aatacgattt taactagaac aatagattaa
    58981 tgtaatattt aatataaaat aattataata attgaaaagg aagtaatcta tgatgaaaat
    59041 agcagtcata acaaccgaat ttttaaaaga gtttgtagat aattctataa aaaagttaaa
    59101 tattaatgca gaaattgaaa tatatatcta tagagatttt tctcatgttg gagatttata
    59161 tttagaaata gaagataggt ttgatggatt tgcagttagt ggccccatcc ccaaaaaagc
    59221 tattacaaaa aaggctggaa ctattaaaaa acct
//
LOCUS       DAGQWR010000004        54786 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.004, whole genome shotgun sequence.
ACCESSION   DAGQWR010000004 DAGQWR010000000
VERSION     DAGQWR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.004"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..470
                     /locus_tag="MIW95_000165"
     CDS             <1..470
                     /locus_tag="MIW95_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149525.1"
                     /translation="DIKESSKNIYNATESQFKIRDYIKNINAGFALYERSAQDLLTKL
                     MTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSLDIQDKLD
                     VMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDELNE"
     gene            463..1095
                     /locus_tag="MIW95_000166"
     CDS             463..1095
                     /locus_tag="MIW95_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBY3149526.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSKIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            1424..2581
                     /locus_tag="MIW95_000167"
     CDS             1424..2581
                     /locus_tag="MIW95_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY3149527.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     FNSEEIVYFTPKGKSYHSIKNCSGLSRSKKILSGTIEESKKNDPCDICYGK"
     gene            complement(2729..3364)
                     /gene="lexA"
                     /locus_tag="MIW95_000168"
     CDS             complement(2729..3364)
                     /gene="lexA"
                     /locus_tag="MIW95_000168"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBY3149528.1"
                     /translation="MYLDLTEKQVLILEFIKSQITLKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(3468..4595)
                     /locus_tag="MIW95_000169"
     CDS             complement(3468..4595)
                     /locus_tag="MIW95_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBY3149529.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(4917..5762)
                     /locus_tag="MIW95_000170"
     CDS             complement(4917..5762)
                     /locus_tag="MIW95_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149530.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(5792..6163)
                     /locus_tag="MIW95_000171"
     CDS             complement(5792..6163)
                     /locus_tag="MIW95_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBY3149531.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIEDACKILSCNGYEVE"
     gene            complement(6316..6576)
                     /locus_tag="MIW95_000172"
     CDS             complement(6316..6576)
                     /locus_tag="MIW95_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBY3149532.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(6765..7709)
                     /locus_tag="MIW95_000173"
     CDS             complement(6765..7709)
                     /locus_tag="MIW95_000173"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBY3149533.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(7730..8584)
                     /locus_tag="MIW95_000174"
     CDS             complement(7730..8584)
                     /locus_tag="MIW95_000174"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBY3149534.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKELLY
                     QILAMHKK"
     gene            complement(8599..9960)
                     /gene="accC"
                     /locus_tag="MIW95_000175"
     CDS             complement(8599..9960)
                     /gene="accC"
                     /locus_tag="MIW95_000175"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBY3149535.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(9976..10428)
                     /gene="accB"
                     /locus_tag="MIW95_000176"
     CDS             complement(9976..10428)
                     /gene="accB"
                     /locus_tag="MIW95_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBY3149536.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(10698..11144)
                     /locus_tag="MIW95_000177"
     CDS             complement(10698..11144)
                     /locus_tag="MIW95_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149537.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            11419..11697
                     /locus_tag="MIW95_000178"
     CDS             11419..11697
                     /locus_tag="MIW95_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149538.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(11826..12572)
                     /locus_tag="MIW95_000179"
     CDS             complement(11826..12572)
                     /locus_tag="MIW95_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149539.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLDFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(12701..13345)
                     /gene="trmB"
                     /locus_tag="MIW95_000180"
     CDS             complement(12701..13345)
                     /gene="trmB"
                     /locus_tag="MIW95_000180"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBY3149540.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(13483..13803)
                     /locus_tag="MIW95_000181"
     CDS             complement(13483..13803)
                     /locus_tag="MIW95_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBY3149541.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            complement(14256..14681)
                     /locus_tag="MIW95_000182"
     CDS             complement(14256..14681)
                     /locus_tag="MIW95_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY3149542.1"
                     /translation="MHDELKIKMYSFTMDCKDPHELAKFYAALLNWEIAFYNEEYACV
                     GAPGTNQGTYPGITFQRNSEYKPPVWPEKPESQQQMAHMDFAVNDLEEAVQYEIHCGA
                     TIAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(14834..15295)
                     /locus_tag="MIW95_000183"
     CDS             complement(14834..15295)
                     /locus_tag="MIW95_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBY3149543.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            complement(15333..16166)
                     /locus_tag="MIW95_000184"
     CDS             complement(15333..16166)
                     /locus_tag="MIW95_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3149544.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCMQLKNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMYICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            complement(16439..17125)
                     /locus_tag="MIW95_000185"
     CDS             complement(16439..17125)
                     /locus_tag="MIW95_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3149545.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALVGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            complement(17140..19617)
                     /locus_tag="MIW95_000186"
     CDS             complement(17140..19617)
                     /locus_tag="MIW95_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY3149546.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDVLTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIENVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDVVMIQIRDLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKKRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(19837..21240)
                     /locus_tag="MIW95_000187"
     CDS             complement(19837..21240)
                     /locus_tag="MIW95_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3149547.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIIFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLTLANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGSK
                     FVGGKSIVFSIIGIIFLIILISGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNKYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            21739..22599
                     /locus_tag="MIW95_000188"
     CDS             21739..22599
                     /locus_tag="MIW95_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY3149548.1"
                     /translation="MKKQKSPILLEMLGLFLGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(22839..23396)
                     /locus_tag="MIW95_000189"
     CDS             complement(22839..23396)
                     /locus_tag="MIW95_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3149549.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(23586..24473)
                     /locus_tag="MIW95_000190"
     CDS             complement(23586..24473)
                     /locus_tag="MIW95_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY3149550.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            24592..25791
                     /locus_tag="MIW95_000191"
     CDS             24592..25791
                     /locus_tag="MIW95_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBY3149551.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(25985..28540)
                     /locus_tag="MIW95_000192"
     CDS             complement(25985..28540)
                     /locus_tag="MIW95_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY3149552.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYIVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYLQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKRGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTGEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKS"
     gene            complement(28537..29223)
                     /locus_tag="MIW95_000193"
     CDS             complement(28537..29223)
                     /locus_tag="MIW95_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3149553.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            complement(29300..30208)
                     /locus_tag="MIW95_000194"
     CDS             complement(29300..30208)
                     /locus_tag="MIW95_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3149554.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKSEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(30210..30881)
                     /locus_tag="MIW95_000195"
     CDS             complement(30210..30881)
                     /locus_tag="MIW95_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3149555.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKACLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            31143..32207
                     /locus_tag="MIW95_000196"
     CDS             31143..32207
                     /locus_tag="MIW95_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBY3149556.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            32365..33051
                     /locus_tag="MIW95_000197"
     CDS             32365..33051
                     /locus_tag="MIW95_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3149557.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINLFKQKVKAFLNHLTTKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            33041..34072
                     /locus_tag="MIW95_000198"
     CDS             33041..34072
                     /locus_tag="MIW95_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3149558.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFNISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDLSHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            34154..34819
                     /locus_tag="MIW95_000199"
     CDS             34154..34819
                     /locus_tag="MIW95_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3149559.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGSLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            34832..37249
                     /locus_tag="MIW95_000200"
     CDS             34832..37249
                     /locus_tag="MIW95_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3149560.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNLSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKTISLQGNILSIIFIPLGAI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKQAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     SYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLIRNIEKKSL
                     TERLRSQ"
     gene            complement(37430..38221)
                     /locus_tag="MIW95_000201"
     CDS             complement(37430..38221)
                     /locus_tag="MIW95_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY3149561.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(38254..38841)
                     /locus_tag="MIW95_000202"
     CDS             complement(38254..38841)
                     /locus_tag="MIW95_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY3149562.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(39018..40409)
                     /locus_tag="MIW95_000203"
     CDS             complement(39018..40409)
                     /locus_tag="MIW95_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBY3149563.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(40599..41897)
                     /locus_tag="MIW95_000204"
     CDS             complement(40599..41897)
                     /locus_tag="MIW95_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBY3149564.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSTERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKLLSSDLNN"
     gene            complement(42258..43133)
                     /locus_tag="MIW95_000205"
     CDS             complement(42258..43133)
                     /locus_tag="MIW95_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBY3149565.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIKEYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(43326..44012)
                     /locus_tag="MIW95_000206"
     CDS             complement(43326..44012)
                     /locus_tag="MIW95_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBY3149566.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(44061..44594)
                     /locus_tag="MIW95_000207"
     CDS             complement(44061..44594)
                     /locus_tag="MIW95_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBY3149567.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDA"
     gene            complement(44638..45270)
                     /locus_tag="MIW95_000208"
     CDS             complement(44638..45270)
                     /locus_tag="MIW95_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBY3149568.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYNTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(45360..46439)
                     /locus_tag="MIW95_000209"
     CDS             complement(45360..46439)
                     /locus_tag="MIW95_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBY3149569.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(46572..47918)
                     /gene="pepV"
                     /locus_tag="MIW95_000210"
     CDS             complement(46572..47918)
                     /gene="pepV"
                     /locus_tag="MIW95_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBY3149570.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(47987..49267)
                     /locus_tag="MIW95_000211"
     CDS             complement(47987..49267)
                     /locus_tag="MIW95_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBY3149571.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(49394..49654)
                     /gene="hfq"
                     /locus_tag="MIW95_000212"
     CDS             complement(49394..49654)
                     /gene="hfq"
                     /locus_tag="MIW95_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBY3149572.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(49768..50703)
                     /gene="miaA"
                     /locus_tag="MIW95_000213"
     CDS             complement(49768..50703)
                     /gene="miaA"
                     /locus_tag="MIW95_000213"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBY3149573.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDIDAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTARWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(50741..52708)
                     /gene="mutL"
                     /locus_tag="MIW95_000214"
     CDS             complement(50741..52708)
                     /gene="mutL"
                     /locus_tag="MIW95_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBY3149574.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKDKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(52719..>54786)
                     /gene="mutS"
                     /locus_tag="MIW95_000215"
     CDS             complement(52719..>54786)
                     /gene="mutS"
                     /locus_tag="MIW95_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBY3149575.1"
                     /translation="NSSEYMVLDMFTRTNLELTQTIRGNKKKGSLLHVLDKTSTAMGG
                     RLLRKYVEEPLINKSKIENRLDVIEEIKDDFILREDLNDILKNIYDIERICGKIAFER
                     VTPKELIHLKNSIEKLPNLKDTINLSNAKILKEYVSEMDKLDDIYNLIDEAILEEPTI
                     TIKDGNIIKSDFSDELKELREISKNGAFLVKEIENREREKTGVKSLKIGFNKVFGYYI
                     EITKANFKQAKLDETYIRKQTLSNAERYITPELKEIEEKILHAEEKIKSLEYEIFVEI
                     RDTIYKNIDRIQKVAKTIANIDVFVSLATVAHINNYVKPAINENNKLDIRNGRHPVVE
                     NIVGEENFVPNDTYLNRGENIINIITGPNMSGKSTYMRQTAIIALMAHIGSFVPAESA
                     DIPILDRIFTRVGASDDLSQGQSTFMVEMNEVSLILKNATERSLVILDEIGRGTSTYD
                     GISLAWSIVEYIQKNIRCKTLFATHYHELTDLEEEFKEVKNYSIAVKEDGEGIIFLRK
                     IIPQGADKSYGIYVAKLAKLPDEVIERAKYILKDLEKNHVYNSVAINGDKENNNIINS
                     NLDEELVKVNQSNFKNKYESLKIEHELIVKDYKHIKKDYDKLNSKFKALNDEVALMKQ
                     NDDKEKINQEDSVKEVALTQISFDSVNRDILSEEILNLDILNMTPLDAINSLYNLQRK
                     AKEIKS"
BASE COUNT        18788 a         8508 c         6358 g        21132 t
ORIGIN      
        1 ttgatataaa agaatcttca aaaaatatat acaatgccac tgaaagccaa ttcaaaatta
       61 gagattatat aaaaaatata aatgcaggat ttgctctata tgaaagaagt gctcaagatt
      121 tattaactaa attaatgacc catgaaaata aaatctctca aaatcaaaaa atccttttag
      181 atgaaaagtt taccttagat gactcaataa aaaatctctc aagtattatt gaaaactttt
      241 ctaatgacct acaagttaaa ctggatatga tgtttgaaaa ttctttagat attcaagata
      301 agttagatgt tatgtttaat aactctttta tgaatgatga agttcctctt gatagtgagg
      361 aattatttaa ggatgatgtt aataatgtat ttaatccttt tagtgaagaa atttatgaga
      421 tagaggataa agaaataaag gtcataggtg aggatgagtt aaatgaataa taaatataga
      481 agaactgtta acagagaatt tgaaaagagt agcttttggc caacatttac agatttatta
      541 tctactgtat tgatggttgt gattcttatc ttatttagct cagaaagtat ttcaggttct
      601 gttgaacaag atttagctaa gaatgtcaat gcttctgttg aagaaacttt taaaaaaagc
      661 aaaatacctg tgaaggtaga taaatctaat gggcaagtaa cttttggtga aaaaaccatg
      721 tttgatgtag atagtgatgt tttaaaacca gaggctaaag aaatgctaaa aatgtttgtt
      781 ccaaagtaca tagagactat ttataaagat tatggtgact atatatccaa aatagttata
      841 gaaggtcata cagatgatgt gggaagttat atatacaact tagatttatc gcaaagaaga
      901 gcatatagcg ttgctaaatt tatagtggga gatgaaatag gtgactataa atacaaagat
      961 aaagtgacta agcatattat tgcaatagga aggtcaaaag ctgaactcat caaaaatggt
     1021 gacaatagcg tgaatagaga tgcatctaga agagtagaac taaaatacga aataaatatt
     1081 aatcaaaata aataattaaa tttttataaa aaaaaagaag gcagttggtg tgagctttaa
     1141 gccttcttta aggaaaaagt ttatttattt ttgttgatac ttaacatatt atagttttta
     1201 gaagcagttt gcaatatatt aaacgtaaat ggtcgtttaa aatgcataac tggatactac
     1261 tgatgtaaaa tatttcattt tatataatat aaaaattata cagtgtcaat tttctatatt
     1321 attgtactat aatctattat ttaaaatatt atgtaaaaaa atatcataaa aacgcatatc
     1381 atgttataat tactagagta ttatgaatat ggaggcgtag tttgtgatag aaataagtat
     1441 aaaaaataaa aaaaattcta aaattaaaac tttgatactt ttattgatag tatctatatc
     1501 gatttattta actggttgta ctacatcaga taatggaaat aatcaatcaa ataataatga
     1561 ttctttgtct agtagtatat caagtgataa gaatgtaaaa attcatttta ttgacactgg
     1621 aaatagtgat gcaatattga ttcaagatgg taaaactttt acattgatag atgggggaga
     1681 taatgatgat gagggattaa tggtagatta cctaaacaat caaggtgtaa aagatattaa
     1741 atatcttata gctacccatt ctcatgcaga ccatttaggt ggacttgatt ctgttgtcaa
     1801 aaactttaat atagaaaatg tttttgtatc gaatggttca gcagaaacaa agagttatag
     1861 ggactttata aatgctttag caaataaggg tttatcccca agtgtaccgc ttgaaaataa
     1921 taagttctat ttagaagatt cttattttga agttctaaat acaaatgggg gagatactac
     1981 taatgaacaa tctctagtcc ttgtatatac taatgggaat gataaagttt tatttacagg
     2041 tgatgctgaa gaaggtaccg aaaaagaaat actacctaag cttgagaaag tagacctttt
     2101 aaaagtagct catcatggtt ctcgtagtag ttcatctcaa gagtttttag ataaagtaaa
     2161 tcctgaatat gcggttcttt tagtaggcaa gggtaattca tatggtcatc cacatcagga
     2221 gacaatgaat aaattgaaaa aaatgggagt aaaagtacat agaagtgatg agtgttcaga
     2281 tataatattt gaatcaacag gtgatggagt atttacaagt tgtaaagatg gaagttataa
     2341 taaaggtgtt agagaagatg gaaactctgg aaaatcaaac agtaatacaa gtaaaaatga
     2401 ttcttttaaa aataaacaaa atacattcaa ttcagaagaa atagtgtatt ttactccaaa
     2461 aggtaagagt tatcactcta taaaaaattg tagtgggctt tcaagaagta aaaaaatatt
     2521 atcaggcaca attgaagaaa gtaagaaaaa tgacccatgt gatatatgtt atggaaaata
     2581 acaaaaaaac aaccaattta tctataaagc ttctgaactt taaatcattt aaaaaataaa
     2641 ataaatgcat aaaaaaaata agctataatc aatagataat acatttcatc tatacgaata
     2701 gcttattttt ttaagtattt tttaatagct atttaataac tctaaataca ccagttacaa
     2761 tacctacaat ttcaacttct gagtcattga gcataattgg ttccataaaa tcattttctg
     2821 gctgaagtct tattaaattt ccttctttaa aaaacctctt aactgtagca gattcaccat
     2881 ttataagtgc aactactatc tgagaattta aggccgtatt ttttttgtct actacaacgt
     2941 aatcttcatc taaaattcct gcattaatca tactttcgcc ttttactctt aatacaaaat
     3001 tgttactgcc ctttactaaa ctggcaggga atggaatgta ttcctctatg ttttgttgag
     3061 ctaaaatggg ttctccagct gttatttgac ctactaaagg aagctctatt atttcttgat
     3121 tagctccaga aacatcattt actttactac gttctaaaac ttcaatagct cttggtttag
     3181 tagggtcctt tcttatgtat cccagttttt caagtttatt tagatgagag tgcacagttg
     3241 aagtagatct taaccctaca gcagtacata tttctcttac agcaggtgga taacctttta
     3301 atgtgatttg agactttata aattccaata tcaagacttg cttttcagtt agatctaaat
     3361 acataacatc actcatttcc aaacatattt tgtttttatt tccatacact ttatattttt
     3421 aattataaca taatccattt atatctgcaa acatttgttc ctgattttta atcgtcctca
     3481 atccattcaa aattcttgac tatatcacgt tttacatttt tcttatgagc tgcataaagc
     3541 tgagtagttg tgacattatc atggtcaagt aagttttgaa catcatatat agaaaatcct
     3601 gtttggatta gagaagttgc agccgttgct cttaatttat ggggactata tccattgtct
     3661 ctagtagtat caagtggaat agatgtatat ttttttacca gtgttcttat agcctttgct
     3721 gtaattctct tattttgaag agacaaaaat aaagcatctt tttcttcatc atttaatagt
     3781 tcatctcttg ttctttcgtt ttgcaaataa tcttttatta catgttcaca agtatgattt
     3841 ataggcatta aaacttcttt cccacgtttt ctatatattt taaattcacc tcttgaaaaa
     3901 ttaaaagagg agatattgag ttctctaagc tcatttaatc taagcccgta tgtaacaaat
     3961 aatgctagta tagctttgtc acgtaattta gtttttctcc aatatacctt ttctttctct
     4021 gtaagccctt cacctgtttc tactgattca agcatcttag caacttcatc tatctcaagg
     4081 cgcttaatag catctggctg aggtttagga agttttattg gattgaatcc atcagttata
     4141 ttattatcta tctgagagtt tctgtataag aatttaaata gagtagagat ggatgatttt
     4201 tttcttgcta acgctctatt gttattttca aaaatcaatg tgtttttttc tgtatttttg
     4261 taatatctac tgcaataatc acctaagaat aaatttatat ctcttgattt tattgtatta
     4321 aaatcatctt ctgtaatatc ttttacacaa tctgcatttg ttaattcttt ggtttcaatc
     4381 aaatatgaac aaaaaaaatt aatatcttct aaataagcaa gtcgtgtaga aactgcaaca
     4441 gaacctttta aatatataaa ataatccttc ataaatacag gaagtttatt ttccaaaagt
     4501 agacattttt ctatgagttc attttctttg ataacaatat tatcaggttt atctgattta
     4561 ttatgtactt ttataatttt cttatcatta ttcaaaatta aacctcctaa aactaaaata
     4621 taaatctttt atattatttt ttgatattta taccaagtga gtataaatat ttaatttaat
     4681 cattttttac atttttaaga gattagcagt gctttagatg aaattatcac taactattcc
     4741 ctaaatccac atttaaagac tagttgaatt taagtactct ttttttattt ctcaatatat
     4801 ttatagaaat attttaaaat atattgagaa attttttatg ctctactaaa atctttttta
     4861 tataatattt ttatatattt ctaacgaaac caaatattag taaaatatgt atttatttaa
     4921 ctaaagtttt taaatgggct gactaagctt aaaatatagg atattatgag agttactatt
     4981 gaaaaaataa accctattgc cataaagaaa cttataaata tgttatactt aactattaaa
     5041 ctggatatat tcaagttatc aaatccaaat aagtatgtaa ttgaacttat tacaaaaata
     5101 actataacta taaagctcag accatttaag ctatttttta aatcagcact actaagtgac
     5161 atatgtgtag atatagaata cattaaaaac ataaatataa aatacttcaa tgaatataca
     5221 ctaggtgtta acaacattat tcttataaaa ttggaaatat cacctaaaat aatgtgtata
     5281 aaaccattta tatttaaatt attaatcata cttacatata actttaaatc tatattttga
     5341 gtcactttca tataactatc tggcagtaat atcttaaaaa ttaaaataat cattattgtt
     5401 ccacctataa taggtgcaat tcctataaag aaatttccta tactttgata aagattatct
     5461 tttttatagc tatgacttac atatcctagt ataccatcat ttttactttc tattggtctg
     5521 aaaaacttaa ctgaatttat tttatgcatg aaaatcaagc acattatcaa atgactcaat
     5581 tcatgaacaa acgttcctat agctccagtt acaataattc cagtttttcc aaaagatgaa
     5641 aagataaatt tattatttag attttctgta atgctaaaaa tcaatccaaa tacaataaat
     5701 attcctatga aacaaaaaat atttattaca cttattttta aaatttcgat tatataaatc
     5761 ataagttaag tcctcatgta aaagtattta actactctac ttcatatcca ttacagctta
     5821 aaattttaca agcatcttct atatcttttt cttttactaa gatatagtct gtatcatacg
     5881 tagaaattgc aaatatgctt attttgttgt ctgctaacaa actacttaac ttagataaaa
     5941 ttcctacaag tgaaaagtct aaaggaccta gtatcttaag tattctccac tccttttctg
     6001 acttaacatc agaaggaata ttattattta aacatacaac acttaattca tcatcagttc
     6061 tagttataga gtaaaacttc tctgtatcta tccatgttgg tattttactg tcattattta
     6121 atctacatac tgcatactct tctaccaata attttaattt cataatgtat atttcctcca
     6181 atcttatttg tttacttaag attatacatt aaaagttaca taaaataaaa catataaagt
     6241 aaaaaaggtt cactatagtg tcctatttct atagccaacc tttaaaactt aattaaattc
     6301 taataaagag atatattatc taggttcaat tataagttta atggctgttt tcttatcacc
     6361 ttcgatttct atatcagtaa atgcgggaat gcaaactaaa tccattccac taggtgcaac
     6421 aaaacctctt gctattgcaa ttgattttat cgcttgattt agagctccag ctcctatagc
     6481 ttgtatttct gcaacgcctc gttctcttag tactccagcc aaggctccag ctacagaatt
     6541 aggattagat tttgatgaaa cttttagtac ctccatttca acatctcctt gttaatttgt
     6601 taaagtatcc attacctaaa gtactaaatc ataattgcca tttactaatt acaatcatca
     6661 tttaaagtat acttttataa aaatatactt ctataatcgt tttaagaaaa cctttatttt
     6721 ttttaaccat tttattatga aaaaactaca aatttcttta tattctataa gcattttcca
     6781 atatttctaa attttttata tctttgagct ataatttcct ctttatccat atctttgtaa
     6841 tgatttaatc tttctaaaat ataagctttt atttcttttg aaacagcttc tacatctttt
     6901 tgtgctcctc caagaggttc ttttataatc ttatctatta tttttaaatc atataaatca
     6961 ttacttgtaa gcttcataac atcacaagct tctttagctt tagatgaatc tttaaataga
     7021 atactagaaa gcccttctgg agaaatcaca gaataaactg aatgttctag catacaaaca
     7081 tcatttccta ctccgatacc taaagctcct ccactaccac cttcaccaat tacaaaagtg
     7141 attactggaa cagaaagttt actcatttca agtaagtttc ttgcaatagc ttctccttgt
     7201 cctctttctt ctgcttctaa accacaaaaa gctcctgatg tatcaataaa cgttattata
     7261 ggtctattaa atttttcagc ttgtttcata agtcttaatg cttttctgta tccttctgga
     7321 tgaggcattc caaaatttct ttttatattt tcttttgtat ctcttccttt ttggtgtcca
     7381 actatagtaa cattaaactt tcctatctga ccaatccctc caaccataga agggtcatct
     7441 ttaaaaagcc tatctccatg aagttctaaa aaattagaac aaatatgttc tatgtattcc
     7501 aatgtcgtag gtctctttat atctctactt agtgttacct tttcatatgc tgatagatgt
     7561 gaaaaagcat cttccttaag cttttccaat ttctcattaa gtgaatttat tttatcactt
     7621 aaatctatat tatttgcctt agaaatagtt attaattgtt taatatcatt ttctaagact
     7681 tttatttttt cactattatt ttcaatcaaa acagttcctc ctttatgccc tatttcttgt
     7741 gcatagcaag aatttgatat aataattcct tcatttttct tctatctact atcatgtcaa
     7801 taaaaccttt ttcaagtaaa aactcagaag tttgaaatcc ttcaggaagt ttttgattta
     7861 ttgtttgctc tattactctt ggacctgcaa aacctatcaa cgtatttggc tcagaaatta
     7921 taatatcacc aagcatagca aaactagcag ttactcctcc agtagtaggg tcagttagaa
     7981 ctgatatata tggcaatgat ttttcttcaa gcttagaaag tgcttgagaa gtctttgcca
     8041 tctgcataag agataccata ccttcttgca ttctagctcc tcctgaagca gaacaaataa
     8101 ttataggtaa attattttca gcagcatact ctattgaata agttattttt tccccaacaa
     8161 tagaacccat acttcccatc ataaaatcag gattcattac acaaataact gttttaattc
     8221 catttattct tccataacct gtgacaacag cttctgactc ttttgttttt tccttataag
     8281 agtctacttt ttctttgtat ttaggaaaat ttagtggatt ttgaaattct acatcagaat
     8341 taaactccat aaaagttcct ttatcaatca ataaatttat tctttttctt gctcttaagc
     8401 cataatgtct tgaacatttt ggacatacaa atgaattttc ttctatgtcc tttctaaata
     8461 catgactatc acaacctttg caataggtcc aaaatttatc atctacacta ttctttttaa
     8521 agttgtcatc taaagaaatc gtaacatact tagattcttt tgatgataaa aattttttta
     8581 tcataatcta tttactcctt ataatgtagc aaattctttc ttcataaaga catcttctat
     8641 aaatttggta tgatgagtat cttctctaaa ctcctcactt gttacaagtg ccttttgaaa
     8701 ttctacattt gtattaatac cttctacaat aatttcgtct agagctctat atattctact
     8761 tatacattct tctctagttt cagcccaaca tattaactta cctatcatag aatcatataa
     8821 aggtggtata gtatatcctg catatacaaa agtatcaaat ctaacaccaa aacctccagg
     8881 taaatttaat gattctatct tacctggtga tggagcaaaa ttctttgatg aatcttctgc
     8941 atttattcta cattcaatag catgacctct aaaagtaata tcttcttgtt taaaaggaat
     9001 tttatcccca ttagctatag atatttgaag ttttataagg tcaagatttg ttaccatctc
     9061 tgtaactgga tgttcaactt gtattcttgt attcatctct ataaaataaa agtttttatc
     9121 tttgtcaact ataaactcaa tagtacctgc accgatataa cctactcctt ttactatatc
     9181 tactgcaatt ttatgaagtt tttgtctttc ttcatctgaa aggtatggac ttaaagattc
     9241 ttctattact ttttgatttc gcctttgcat agaacaatct ctatcaccta aatgtattgc
     9301 atttgaatgt ttatctccaa atacttgtac ttctatatgt ctaggatttt ctataaactt
     9361 ttccatatat aaatcactat tagaaaatgc tgctagagct tctgaacttg ccatatcaaa
     9421 atttgaaaaa agttcttctt cttttctaac gattctcata ccttttccac cgcctccact
     9481 tgaggctttt atcataacag gatatccaat ttctcttgca acttctaaag cttcttcctt
     9541 agttttcaaa acagattctg aaccaggaac aacagggata ttcaattcca tcatagtttc
     9601 tcttgctcta gatttatctc ccatcaaaat catttgttct cttgttggac ctataaaaat
     9661 aatattatta tcttcacatt gctttgcaaa ttcaggattc tctgataaga atccaaagcc
     9721 tggatgaata gcatcacact ttaatttaag agcaagatag attatatttt ctatattatt
     9781 ataactctta cttatattat ttggtcctat acaaatagat tcatcagcta tatatctatg
     9841 aaaacaatct ttatctattt cagaatagat agcaacagtt tttataccta gttctttaca
     9901 tgttctgatg attctaactg ctatatcgcc tctatttgca actagtattt tttttatcaa
     9961 cttaaatacc tcctactaca atggttttat cttaaataaa ggttgattat attctactag
    10021 ttcttcatta ctaactaata cttcaattat ttcaccttta atttcgcttg ttatctcatt
    10081 cataagcttc attgcttcta atatacataa agtgtcgcct tcttcaacta catcgccaac
    10141 tttaacaaag ctatctgcat ctggacttgg agaatcatag aatgttccca tcaaaggagc
    10201 tactactact gatagattct cattagatgt gacaggtgta tcgtttacaa ctggcttttc
    10261 taaagaaaga tccactactg attcttgttg tacacttaaa acaggtgaac ttgtagattg
    10321 agctttttta gatacttcta atcttaaatc gctactttca agttttacat attctaagtt
    10381 agttgagtct atggccttta ataattcctt aatttcattg atgttcattt tgttaccccc
    10441 ttgtattatt acctggtgct aatattatat cacaaaaaac aaatttataa aagaaaaagt
    10501 gctgactaga gaatcaacac atcaatatat cttttaattt taaattaaca accagtaact
    10561 tatatttgtt ttaattttcc attattaata taaattataa gtataaaatg aataaaattc
    10621 aatatttttt gcaaaataca agagaataca aattaatacc tcgggaattt ttaagacaaa
    10681 taccaatatt atgcaattta gacttcgctt tctttcaaat tagtatcatt ttctttattt
    10741 ttacatagga tgtactttaa tttagctatc aaaacattac tatataggtt aaaagttatt
    10801 agaattgcag gaattataaa gtaaactcta gcacctataa aaccaaaaac tactgtaata
    10861 agtattaatc ttttatttaa agtaaaaaaa ggtatattaa aaatactttt gtcggatgtt
    10921 tctggagtat aagaatatat aactccaaaa atcaaagtaa gcatacaaag tggaagtagt
    10981 gtataacttg aaattattcc cagaaatcct atactttcaa aatatttgct agcactattt
    11041 gtataaacaa acatgtatcc atttaaatat gcgcatatac atgatataat gccaaaagtt
    11101 ccccctaagg attgagtgta ccaattaaaa aatttagatt ccatatacct cacctcctaa
    11161 aactgtatat ataatgatat gcatttaaat agtaaaatat atctttatat gtttctgaat
    11221 aaaattttgt taatttcatt aatttatgtt taagaaattt gaagtatagt gtagtatata
    11281 ttaatgtaga tgaagtttta tagtacttat ttcatagtgc ttaatcaagt tatcttatta
    11341 tggattcaaa agttgtttgt agggttttat attttgttga gctgaaaaat ttattataaa
    11401 tgaagggatg ttttcgcttt ggtagataca ttagaatttg gtcttaaaat tttatttttt
    11461 atattatcaa taatttggat gggtaaaata atgattttaa gaactgataa gcaaatagta
    11521 atcaatcctt tgcttattgg tatatctgca gtccttgtga tgttgcatac ctcacagagt
    11581 aacattgaat tttttggatt agatgtccaa tatataagaa tagttttgta tataatatat
    11641 tcactgataa ttttaatagg aatatgggct actaacagaa gaaatggaat tttttagata
    11701 atctatttct ataaaattta aaggttgcta gtaacaaagc actaatatta gcgttctgtt
    11761 actaacaacc tttattttga ttatacactt aaattgaaaa tagaatgatt gaataagttg
    11821 acatattatt ttacattgac agtgattaga acttcttgag atgtatttaa atcatttttg
    11881 tcgcttagta taacttttac cacagcttga cctgttttat tacctttaat tatcatatct
    11941 tcacatgaaa gtacactctt atcacttaca gatacaataa agtctaaatc ttttggaaga
    12001 ttatcagtaa cttcccagta tttataataa ctatcaattt tcttcgtaat tatttttctc
    12061 aaatcaatac tttcaccttt tgctatatca acattatgat atttagagtc cagtttatac
    12121 tgattatctt ttgtatcaat aaaatttaaa tcgcaattta catccacacc attatgagaa
    12181 agagaattta ctacatcgtc atcaatcaaa taattattgg caagatttat tgaatccata
    12241 tcttttagac ttaaagcttc cttaggaacc tcttttatat tattattcca aaaaataaaa
    12301 ttgtctacat tttcaagata tgatattcct tttaaatttg aaatgctctc accagttgca
    12361 taaaattctc catctaaagc ctttatattg cttatagtca tctcatctgg gtttatatat
    12421 ctttttacaa ctgctcttaa atttgcatca ggaaaaatat ctttaagttg attttcgctc
    12481 aaaaccttat catttgaatt tattggactt aaagctgctg cagaaataat aaatggtatt
    12541 aaattagcta aattcatagt accctcccac ataaattttt tattataata atttaatttc
    12601 tatgagaggg ttctgtttcc ttgttatata tacctacttt cgtacacttt attaatataa
    12661 attaaaataa atttctacta ttaaacttat aaacatatta ctagttacgt tttttagctt
    12721 cacatctaaa tattcgcata ccttgtgaca taaatttgtc ttcatactct gttgtaacat
    12781 tattttcata ctcactattt cctaaatcaa gagaaatatt ctttaatagc caattatttg
    12841 cagcaatttc atttaaacta aactcgaata atttttcatt atctgtttta aagtgaattt
    12901 cgccatcatc ttccaaaacc ctattgtaca tttctaaaaa tccagaatga gtgagtcttc
    12961 ttttactcca tctattttta ggccatgggt cacagaaatt tatataaatt ctcgataatt
    13021 ctttagcttc aaagtaatcc aaaatattgc ttacatctcc ccataaaaat agtatattat
    13081 tcaaattaga agcatctgct ttttcaacag cttttaaaag aacttcttcc ttaagttcca
    13141 tggcaatata atttatgtca gggttctgct tagctaaagt tgttataaac ttaccttttc
    13201 cagtcccaaa ttcaacatgt attggattat catttctaaa ctttaaattc cacttgcctt
    13261 ttaatttgtc tatatcgtct cttaaaacat attttgtata acttaaaagc ttttcatctg
    13321 cacctttttt tcgtcttctt ctcacaataa ttctccttac atataaaaaa ttagtaaaat
    13381 acatttttat tatacattat ctcaccaaaa aataaaaaga actctcttaa aatagaaatt
    13441 tatttctata aagagagttc taaacttact aaaaacttaa tcttagtggc accctgaaca
    13501 accagagcat cctcctccac cttgatcttt catattttca ggaataactc taaatccaaa
    13561 tcctgtatct tctattatta tatctccata ctgactatat tcatcagagc tcattacaaa
    13621 ttgtaattct ccaacttcat aagttaagtc atcttctttt ttctcatcta aagctaatcc
    13681 aaaagaagga cctccacaac caacacctgc aaagtatact cttatattat taggtttatc
    13741 ttgattgtct tttaagatac tttttaaagt atcaatagct gtttctggta aagtaatttt
    13801 catcaaatca ttccctttca aatattttat taaaacaata tctattatat acccatacaa
    13861 ggtacataat caattagttt ttgcatattt tactaaaact tctacctaat attacctaag
    13921 gctttaaaat attttatata atattttatt accttaaata aataccctat cataaactta
    13981 gtaaacaaat aaaattatta ttctaaaaaa tataataatt ttatttgttg ttttaatatt
    14041 ataacacatt tgataatgtc caattcatat atgcaaaatt tatatacttt aaaatatcta
    14101 agaaattatt caataaaaaa atactatttt ttaaaaatta tcattattga taaagctcta
    14161 ctatttatat tttttagtgt attcatgtat aataagtaaa agcacaacgg tatatactgt
    14221 tgtgcttttt tgtaattaat aaacaatatt ttggtctata acaatgaaaa atggctactt
    14281 tccataattg atttcatctg acataagcaa aaagggtgtc cagctgggtc aatcataact
    14341 ctccagtcat cagtaaattg ctcctctgcg attgtagctc cacaatgaat ttcatattga
    14401 actgcttctt ctaaatcatt aacagcgaaa tccatatgag ccatttgctg ttgagattca
    14461 ggtttttctg gccacacagg cggtttatac tcagaatttc gttgaaatgt tataccagga
    14521 tatgttccct gatttgttcc tggagcacca acacatgcat attcttcatt ataaaatgct
    14581 atttcccaat tgagtaacgc tgcataaaat tttgctaatt catgtgggtc tttgcaatcc
    14641 atcgtaaatg agtacatttt gattttcagt tcatcatgca taacgtaatt acctccaagt
    14701 ataaatttct tcgtcttttc atttttaagc taaattcaaa cattaagtat gtaatttatt
    14761 tcaagtattt ttctattatg ccccaagcct ttgttttttg caatataaag aaaaaattat
    14821 atattttaat attttataat tgttgaaatt gtgtaataaa ttcaactgga tacatagtgc
    14881 gttttgaaat ttcttctaaa gctaaacctt gttttaaaag cctctttatg acgctttcca
    14941 tactttctga aatttctata atatttttat caaaatcata aaacctaaaa gttctttgac
    15001 cccatgggta ttcttttata taatgcaaaa attcaatatt tcctattcta tttatcttac
    15061 tttcccattg tttgatatca ttaacctcaa aatacaactg aaagttattt gattgttgat
    15121 atattggtaa atcatatcca attatagctt tataattact ttgtaatcca aatccatttt
    15181 caaatgatac atgttctcct aaatctaatg taattttttg ctccataaca tcttcataaa
    15241 acttttttga tttatttatg tctttgacaa ttagcaatac tcctccaaat ttcatcttat
    15301 cactatcctt tctttgttat aagtattata acttaaaatt ttgcaatttc actgtaaaaa
    15361 tcagacatat tagctcggta tttttgagga gttattccac agacaatttt aaaatcttta
    15421 ataaaatgtg gtatatcata gaatcctaat tcattacata tatacatcaa tgaagtttta
    15481 ttattgttta gaagttttat agatttatta atacgtacca aacgagaaaa tgacttcata
    15541 ctcaacccta ggtactgatt aaagagtctg tttaaatgcc tagaactata aaatactttt
    15601 ttagaaattt cttgtgaaga tatgtttcca gagttttgaa taatcaactg aattgccaaa
    15661 ataaactcat atgagtattc aattttaata ttagaaatca ataattcttc caactttaaa
    15721 atcaatttat ctatagataa agattcatta aatatttttt taatttctaa atctaatgaa
    15781 gtattaatta tagaaaatgg aacaacttta tcaataagtt cactttgctg tatacctata
    15841 agcgggaaaa aaccagcagg ttgaaattct ataattagta taacatcaca tctattagca
    15901 atatttccta ctgtttttgg tttagtagtt ggaccaaata atagactatg taaccctgtg
    15961 gcatcataaa aaaacaccaa agttacactt ccatgaggaa taatcgtgta attatcagaa
    16021 attattgttt gatttggaaa cgatatagta aaattcgata tccaattttt taattgcata
    16081 caaggcaaga gataaacaaa attgtcttca caaactataa gtgacctatt tttatttata
    16141 aaatgaatac tttctttttt taacatatat tatatcctgt cctatcgtga atatttatta
    16201 aagtaagaag aatccatatt atctctatat atcattaact gcttttaata atagagatag
    16261 tatgaatact cattagatat ttaataaaca aataaattaa aataatcaat aaaaaatata
    16321 gtacttttat ctgttatttt aatattatag cacatttata gtatttaact tatatccact
    16381 ttacatatgt ctaatttata tagttaaaaa tgcttatctc aaatacaata attagtcctt
    16441 atacaattaa accatctgaa actgtaatag tacgactact acttttggct gcttctggtg
    16501 agtgagttac cataattata gtttgtccat tgtctctgtt tattttattc agtaaatcta
    16561 ttatttctat ccctgtttta ctatctaaat ttccagtagg ctcatcagca aataaaatct
    16621 ctggttttcc aactaaagct cgagctatag caacacgttg ttgctgacca ccagataact
    16681 ctcgtggtgt atgtttacgt ctatcagtta aaccaactat atctaagatt tcatctaatt
    16741 gatttttata atcttttaaa tttttaccat ctaataacaa aggaagcatt atattttcct
    16801 ccacatttaa atttggtatt aagttataaa attgaaatac gaatcctata tttctacgac
    16861 gtattatgct cattttctca tcattaaaat gtgatatatc agctccattc atatacactt
    16921 ttccactcgt tggagtatct aaaccaccta aaatatataa aagagtactt ttaccagaac
    16981 cagattgacc catgatagaa acaaattcac ctttcattac ttgtaaagat acctctttta
    17041 acactcgagt tgttgtattt ccaattttat attctttgat aatatttttt gcttctacag
    17101 ctaaactatt atgtgtaatt ttcatattaa cctccataat tattcaaact taatttcttc
    17161 tatcagtttc attttcttcc cttttataat aggcactatc gaacctatta aagttactat
    17221 aattcccaat aacccaacaa taataaatgt tttaaaatct aaatctggtt tcattgaaat
    17281 ttttggtcct gcaactataa agatagtttg aatctcaagt atagatatca caataccaat
    17341 aacagctcca agtaaacctg aagtaaaccc ttcaatcaat gtcactttta tattttgttt
    17401 atcacttagt cctatagatt tatacatagc tatagaacgt tttttttgaa tataattaat
    17461 cagtagatta ttaataatac ctactgtagc aagaagaaaa atgaaatagg tcatcttatt
    17521 cataggagac aaaaaactac ttatagtatt taaagaatca ttattaaatt cttcaactgt
    17581 acgactccaa ttatatgtat ctccaaataa atctcttatc tgaatcataa caacatctgg
    17641 attgactgct gtatatacta aaaaaccata attagtttta tcaaaatcat taactgcata
    17701 atgtgatggt ataacagctt ctacatcatt tgcacgggat ttaaaactac ctactatttt
    17761 ataagaaaaa tcatacttat cattagatag agttattgta tctcctatag ataagcctac
    17821 tttttttaga gtatcttcat tcaaaataac agaacgttta tcattaaaag cttctattac
    17881 ttgttttttt atctcaaaat ttgtataatt gattccaaac atagagttat atagttttat
    17941 attatctgta ccctctagtc ttgataatgt tacaccatta ccagaaatct cattattcat
    18001 aacataaaga ggtaatattt ttttaattcc atccatatgt ctaacatttt caataaactc
    18061 ttcattcatc tttccatctg caaaaccttg taattctgcg tcacgaaaca catctgttat
    18121 ataagttttg acaaagttac caacaacact tattgcaata actgcagaaa tacttataaa
    18181 tagtaaagtg atattctgta ttatattttt gttatttttc atatttctag cagctaattt
    18241 accctcattc cctaaaatat ttttataaac aaactcaaaa actatagaca tgatatcagt
    18301 aataagagga atcaacacaa tagtagctac aattaatcct agtaatgaaa atccaccagc
    18361 tagatacaat gtattttcag gggatattct tggaagtaaa atagataaaa taaacattat
    18421 acttcctata aaaagtatag aacgatttga aacattcttt tcttctactg ttcctagtac
    18481 aatatttttt attggcaaat ggctagcttt tttaacaggt atatatgcac taaatgaaga
    18541 aacgattatt gctactataa cagatataat cactccaatg ggtgatatta ctactggaat
    18601 tgatattcct tgttcgagag aatttcctaa cccatgaagc attaaattta aaacaactac
    18661 accaatagga atagcaatta atcctcctat acttccgtat aatatacttt caagcatcaa
    18721 tatacgagtt acagtttttt cattggcacc aatacttcta aatgttccaa taaaaggaag
    18781 acgttctaat gtaataacct tatagctgct gtatatgata aatatactaa gggttaatgc
    18841 aaaaaaactt attaaaaaga aaggcattgt cttttgtcta gcatctgaaa ttatcttagt
    18901 ttcatttata gtatttgata ccatatattt ttcagtggaa agttcttcgg ataatttatt
    18961 tactagattt tctgtcattt cttcttctga ctctataaga actcttgtat atccactgcc
    19021 tttatttata atttttgata gagtctcttt gggtagcagt gcatttacac ctcttgtatt
    19081 cctcaaaaat actgtatcat aagatgcaat atcactaact tgaaaagtat aagactttcc
    19141 ataaatctgt aaagtaatag aatcaccttt ttttatttta tattttgatg taaagcgatt
    19201 tggaagaata attttatcac cagaaaaatc agtaatacta tcgccatttt ctaatcgtgg
    19261 cttgttgatt ttatttagtt gacttaagtc tgctgatata agactaaatt cttcatagta
    19321 tcccccttta ttataaatag cagagctttc caatacacca acctttgatt ttatagcatt
    19381 taaatcaggt atgtcttcta aattggttaa tacatctgga tttttacttt ggacagatat
    19441 agtcgctgta ccatacatac cttttgccat ttttctttgt gcactttcat aagataatcc
    19501 aattgagaat gaaacaaaaa gtaaaactgt tgataaaact atggacagta acataactgc
    19561 tgttctaatt tttcgttctt taatatttgt gaaaatatac tttaatataa ttttcaaatt
    19621 tacaccttct tcctttatct aagatatttc agtataatat ttttatactt taatccaaat
    19681 tgaatctcta atagactcct ccatactttt atcattctat ttataatatt tttaatcaaa
    19741 tcacctcctt aaataaaaaa gattgataaa ctactatata aatataataa tctatcaacc
    19801 ttaatatatt ttttatttga ccttaattaa ccttaattaa ataggaaagg tgatataaaa
    19861 tgagctacct aatccaggta tactttcaac agatatattc cctccatgaa cttcaataat
    19921 ttgcttcgtt attgctagtc ctaaaccagt tccttctatt ttttgttttg tattgcttcc
    19981 tctataataa cgattaaaca aatttgatgc ttcttctctt gacatccctt ttccattatc
    20041 agatataatg atttcaattc tttcatttaa tgttatatgt acacaaactt tagtattttt
    20101 atctccatat gtaaaagcat tgacaataag attactaaaa gctcgtttta atagtgtaca
    20161 atcaaaatca aagttaatat tatctatatt actcttaaat tcaactatat ttgattcata
    20221 ttctggataa tttaaaatat caatggataa ttctttcaag aatcttacta aatcatattt
    20281 atttttattt aaaggaatta ttccattttt taattgatag gttaatttta aatcgtttat
    20341 aagtgtattt gtatattcta tattctttaa catgattgaa gcatatttat atactttttc
    20401 aggtgaataa ttagtactac tatctgtaag aagctctgca taccctttta ttggtgaaag
    20461 tggagttttt aaatcatgag ttatatttga aatccactct tctctcataa tatcagtttt
    20521 attttgtatc tcatcacttc tatgaatttc ttcatttaaa atatttaaac tattatatat
    20581 atcagcaaaa acacctttaa tattaactgg aagatagctt cgttttgaaa tatcactaac
    20641 agcagatgta atatttgata tgcactttgt tacccagtaa ccataagcaa ttccagaaat
    20701 caaaattatt aaaaatataa tccctatgat tgaaaataca attgacttac cacctacaaa
    20761 tttacttcca tcaaaaaaca tattcacttt agatatattt aatggaaaat atataatata
    20821 aatatagtca tttttattat ttttaattgt attaaagtat acagttccat tacttgaatt
    20881 attttggtta gctaatgtta ataattcaga attagaataa taatctttat gctcttttgg
    20941 ttctaaaaac ccataaactc tatttccact agaatctaaa atttgcaacc acaattgatt
    21001 ttctcgaagc ttttctaatc cagactgttt tacttgtggc ttattatcta taaatatgat
    21061 ttgttcatta aagttttctg taaatttttt cggccagtca ctacgaataa attccccatt
    21121 tggtttctga atagtaataa gtgataagaa aatacctatt actgtcagga ttaagcaaat
    21181 aaaaatcatg aaaaaaataa tgtatgaatg aaatactgtt ttataactag gtttatgcat
    21241 ttaatcaact cttttcttaa acttttattt tagtttgatt gttgcttata taaaatagat
    21301 aataattatt gatatgtaaa aatttattta aaaatacaac tatctacata aattatattt
    21361 atataattat atgttatcaa gctgtctgtt gtatttaata aatccttttg ataacatata
    21421 aaagcaagat aattttttcc attaaatacc ttataattat acacaaaatc tctatatacg
    21481 ttaaacagta tagagaaaat tttcatagaa ccatctttat aggggatact ttataaaaga
    21541 ataaaaaaat tataatatat aaaaaacttc ataggagtgt aacaacatca gtaaagatat
    21601 gaaaacacta tataaaaatt caaaaatatt aagaataatg aaatacttga aattgcttat
    21661 atcaagaaat tttatataat ataaactggt caaatatgtt ataatattaa attgtgacaa
    21721 gttcatggag gtgtttatat gaaaaaacaa aagtcgccaa ttttacttga aatgcttgga
    21781 ctatttttgg gttgtatatc aatgagtatt ggtataaata tgtttttaaa accacataca
    21841 attgctcctg gaggacttag tggtctatca ttagtactaa acaaggttac aggacttcca
    21901 gtatcagcta tcatgttaat tataggtgtc ccattagtaa tacttgcttt tagaattatg
    21961 ggtacaaaaa attcattaaa aactttattt ggaacagttg tattttcagc tatagttcaa
    22021 ttaacagacc cattatcaaa actaagattt actaatgact tattattgtc ttctattgca
    22081 ggaggaatcc tagttggtat aggtttagga ataatgttta aatcagatgc ttctactgga
    22141 ggaacagact taattgcact catacttagt aagaaattcc caggaataaa agctacaaaa
    22201 ttcatgtcat gcctagatgg aatggttgtt atatcttctg gtatagtaaa tcgtagttta
    22261 gaaactgctc tttactctgg tatagcactt tgtgtattaa taaaaatagc agatatgata
    22321 atggaaggat ttgaccattc aaaagcattt tttattatct ctgatgagcc tgaaagttta
    22381 agacaagcta taactgaaga acttgataga ggtcttacta ttcttgatgg taaaggtgga
    22441 tacactagag aaaataaaga agttctatta gtcgtagttt ctaaaaaaca agaactttat
    22501 ctaaagagat tagttaaaag aactgaccct actgcatttg ttattgttac agatgtgcat
    22561 gaggttcttg gagaaggatt taaaaattta gagaactaat aaaaattaat attttaagta
    22621 tatatactat tttaattaaa atacgattta ttcaaaaaga gtgtctcata aactttttag
    22681 ttcatgagac actctttttt gtataaaatc aaatgcattt tcagtatttt aacaataaaa
    22741 aacaggatta tctatatttt gagtcaacta ctttattgaa attttattta gaaaacaatc
    22801 attactaaat aaccaggata gatattaaat gattatatct attgtttttt caattcatat
    22861 tgataacaag gaacattttt tacagtattt ccagtatgaa tttttttgat atactttcct
    22921 gtacatttaa atcctaataa atctaatgat tttgtagaat tttggttttc ttcataggtt
    22981 attgcaatta ttttatttaa gtttagttct ctaaatccat actgtattac tctttttgtt
    23041 atctcagtag caaaatcatg tccccaatag cttggattta tacatatacc tatttctgct
    23101 tctaatgctt taggatttat attattaaac cccatattcc caataacttt attgtctctt
    23161 ttatatacta tagcaaagtg gccaattttc ccttttttat agggatttag aaaaaatgat
    23221 tttataaact ttcttgtatc ttctaaagat ttatgctcac taaatggtaa atgttttact
    23281 actattttat ttttataaca gtcaaaaaaa tcttcaacat catctaatga tgcaggtctt
    23341 agtatacaac ttctagtttc taaggttttc atttgaggat attcagaatc agacatttct
    23401 aatccccctt tatacattaa tctaaatata ctgttcatat ttaaaatacg atattaatta
    23461 tataaatgtt ctcaaattaa ccttatcttt atttttaata aaaactaatt actatatgaa
    23521 tctttcaaat ttacgaattt atctctattt ttatattaaa ttacttgtga aatatgtatt
    23581 ctaaattata tgttattaga tatcatgctt aaaaattctt tggttgctaa ggataaagat
    23641 atatctttta aatggcagta acctatattt ctcttaggta ttggctcttt tatatctaat
    23701 ttaaacaatt tctcattttc caaataatct atgctaaatt cttctataac acaagacact
    23761 ccaagattta tatacgcaaa ttctaaaagt aattcatgag aacccaattc tatatccggg
    23821 tttagtttta taccttttga taaaaaatat tcatttacat aaagtcttga atttgcttta
    23881 ttttctagca ttattaaagg caatgtgttt aattcttcca atgatatttt cctacctttg
    23941 tattctatgt aatcatttgc acatacaaat atatcatgta tttcaataca ctcaacaata
    24001 tttagtgtat catcttctat tggcatgttt ataattgcta tatctatatt cccatctttg
    24061 agtaaagata taagttctct actcgttcta tttattgtag atacatgtat atgtggatac
    24121 ttcttatgaa aaatttctaa atattttaac aagaaaaaac gtgcagctgt atctcctact
    24181 cctattttca gagaaccgta ttctaatttt ttaaatttat ttattctatc ttctcctgat
    24241 gatatcaact ttaatgcagt agtgacatgt tcacttagaa ttttcccctc tggagttaat
    24301 tttactccct tattgtttct ataaaataat aaagtatcta attgttcttc taattgcttt
    24361 attgactggc ttacagctgg ctgtgacata aacaattcct tagcagcaag agaaaaactc
    24421 ttattattta caactgcatt aaaaacctta taaagttcaa gttttatatt catatataat
    24481 tactccttat gtcaaatatt agtattattt attttactta taattatatt tgaattataa
    24541 tatacatggt actaatatga caatattaat tctttaggag gattttagat aatggataga
    24601 ttagttggaa ctgtttcaag aggggttaga gcacctatta taagacaagg agatgactta
    24661 gttaaaatag ttgttgattc tgttttaaat gcgtcaaaaa gtgaaaactt tgaagttagg
    24721 gacaaagatg taattgccgt tacagaagct gttgtagcta gagcacaggg aaattatgct
    24781 cacgttgaca atattgcaaa agatgtaaaa gataaatttg gagatgatac tgttggggtt
    24841 atattcccaa ttttaagtag aaatcgtttt gctatatgtt taaaaggaat tgcaaaagga
    24901 tgtagaaaag tcgtattaat gttaagctac ccttctgatg aagttggaaa tcacttaata
    24961 agtattgatg aacttgatga taagggaata aatccttggt cagatgtttt aacagaagaa
    25021 aaatacagag aactatttgg atataacaag catacattta ctggtgtaga ttatgttgat
    25081 tattataaga atttaatcaa ggattgtggt gcagaggtag aaatagtatt tgcaaacaat
    25141 ccaaaaacta ttcttaacta tacaacaagt attttaactt gtgatataca tacaagacaa
    25201 agaacaaaga gaattttaag acaaaatgga gctaaaaaag tatattcact tgatgatata
    25261 atgacagcaa gtgtagatgg tagtggttac aatgaccagt atggactttt aggttcaaac
    25321 aaagctacag aagaaacagt aaaattattc cctataaatt gcgatgaagt tgtaaacaaa
    25381 atccaaggta atataaaaga aataacaggg aaagatgtag aagtaatggt ttatggagat
    25441 ggagctttta aagaccctgt aggtaaaata tgggaattag ctgacccagt agtatctcct
    25501 gcttatacaa aagggctaga gggaactcca aacgaagtaa aattaaaata cttagcagat
    25561 aatgattttg ctgacttatc tggtgatgag ttaaaagaag caatttcaaa atacatagtt
    25621 gaaaaagata ataaatctga tgatttaaca ggaaacatgg tttctcaagg tactactcca
    25681 agaagattaa cagaccttat aggttcttta gcagatttaa catctggaag tggagataag
    25741 ggtactccta ttatttacat acaaggatat ttcgataact atacaaaata gtatgtttta
    25801 ataagattta atttttttgg attatatata ttaacatgaa aatttattaa aacctcttaa
    25861 tagatatgac ttttcaagag ttatatctat taagaggttt atttaatgtt cacagataag
    25921 atatatttaa ataatttaaa caagattgtc ataaagttat ataatcttaa aaatttatat
    25981 aactctaact cttatttatt tctgaaataa tatcactctt caaaatttgt cttactggaa
    26041 taattacagc aactatacta atagtgatat ttaaaataat catacctatg tatagaggtg
    26101 tatttatttt aagaattgga tttatatata aatatacacg ttttaccata aaataaatca
    26161 taaattgtcc aataacactt ccaattacca ttataatact tgcatagaat ccatataaaa
    26221 aaccttctct tatcatcata agtaaaaact tattatccgt aatacccata gctcttaaaa
    26281 ttccaaactc atgtcttctt gaaaaaatta agtagcttac tgtattgatt atatggaaca
    26341 aacttattgc aagaagtaca aatacaatac catatataaa taagagtttt tgatttaagt
    26401 aggtattatt tttttcaata gcaatggtat aatctgttac gatacatctt tttacatcac
    26461 ttacaacact ttttatgttc tcagacaatt cctcactgtt actatcttct gtctttttta
    26521 tacttactga attataattt tcgatatcaa agttattttt catttgccca ttcgtcataa
    26581 ctatatccat accataatca ccaatgaaat atatgttatt tgccatcaca cgttttacag
    26641 tagcagcaac tacaaattct tttgttgtat aatcattctt attttcttct ttaaatttaa
    26701 caatttcacc tgttggtttc attgtctttg gcactttaac ttttattgta tctcctggat
    26761 ttatatcaac tgcatcatac atcccagttc catccatagg tagtctaact ataaccttat
    26821 tatttttaac catatcatgg gtattaatct ccccatctat aatataagaa tttaatcctt
    26881 ctaacatacc attgttataa ccataaatat ttgtttttaa aagatagcca ccttcatttt
    26941 gctttgtaca aataccatca aaaaaagtct ttatcctgta atctttttca agaggtttaa
    27001 agaaattttt ccaaaatagt tgttcttctt taatttgcat agctccaaat aaatatctta
    27061 ctggatagac ttttgatatt ccctcaacat tgtttaatct tgaaatttta tcttttggta
    27121 tacctacatc aaaatctgtt gtttgcatag ttatttggta gtcagaattt aaaccatcat
    27181 cagctttcat tgtaagttca ttattgtttt tagtaagctg tatagagtaa ctagaacata
    27241 gaaatataat tccaccaagt gataatgaaa acaaaattcc aataaacatt cccctttttt
    27301 gagtcatgta tttatgagat aatacttttg tcatgttaga gtgttttaca ctttctaata
    27361 ttctcttttt cttattagta atcttaaagt ttttcataga ttctatacta gagtatttat
    27421 tcaactgatg tacaacacgt acagcaatta aaattaacaa caataagata aataaaaagc
    27481 ctaaataaat agatttttca cttataaaaa atgctcctgg aacaatatca ctactcaaaa
    27541 atatatgtgg aaattgttca tatattgtag acgctactcc aatacctaag aagcaaccta
    27601 taggaaatcc aacaataaaa agtaaaaata actctaaaaa tagtagaaat aaaagttgtt
    27661 tattattagc gcctaatact tctaaaaccc catattgtga tattctctga agtattgaaa
    27721 catggaaaat actatatact gctgctgcac caaacactcc aacaagcaat ataattgcat
    27781 ttgtatttag ttctaaattt tttaagaact caaatatatt atatttttct atattctttg
    27841 gtggttttac tccaaattca actgaaagag gccagtttat ataagttttt tcttttgata
    27901 tgttgaattt tttcataaat ttataatttt gctttttaat atttttattt tcatcatatt
    27961 ttacataaac tatataagaa tccttttctt taggctgcaa ataatcgttt acaaatccct
    28021 ccatagcagt ttctgggttt gaaagacttg ataaaatacc acataactta tagattttac
    28081 catctaaatt gatttctgtt ccaatttctt tgtttacatt taaattgttt agagaatatg
    28141 tgtcaagtac tatctctcca tctttactag gaagagttcc ttttaataat tttctacctg
    28201 tcatgtctaa atatgaagaa tcagcattta aaaattctat aataaaaggt tcatctatct
    28261 gatttttagg ttttgtaacg ccaagtctat ctacaatgta ttcctttgat tttttatttt
    28321 ttttaatttt attaagttgt tccttatcta ctttatagaa ataatgataa tctccactat
    28381 cttcccttat cttttcaatt ctacttttgt ctgcactatg aactaaagaa ccaattcccg
    28441 tcaataatgc tactgatagt actatgctta aaactataga tatagattgt tttttataat
    28501 gctttatata ttgaattgaa aattttacta gagacatcat agtacttcac caccagtatt
    28561 caccacaact ccatcttcaa tatggataat tgagtcgcat acttgtgcta tattttcatt
    28621 gtgtgttacc ataagtatcg tttgatggaa ttttgcacta ctttctttta ataaacatac
    28681 cacttccatt gtagttttac tatcgagatt tcctgttggt tcatctgcta atataagtgc
    28741 aggtttattt gccaaagctc tagcaattgc taccctctgt tgttgaccac cagatagttc
    28801 gcttggaaat tttaaacgct tatcccaaat tcccaatgtc ttcatcagca tttcaatata
    28861 ttcatggtct atatggtttc ctttatctaa tgtaacagga agagctatat tatcataagt
    28921 gtttaatacg ggcattaaac tgtagttttg aaatataaat cctatatttc ttcttctaaa
    28981 tactgtcaaa tctttttttc ctattttaga tatgttcttt cctttaatta ttacatcacc
    29041 tgaggttgga ttatctaatc cacctataat atttaacaat gtgctttttc cagaaccaga
    29101 agttccaact atagcagtaa atgtatctcg ttcaatttct atacttactc catttaaggc
    29161 ttttacttta gtctcacctt ttccatagtg tttcactaaa ttattcgtca ctaatatact
    29221 catttaattt ctcctttcaa tacagatata tgttctataa aatattgtaa gtgacgataa
    29281 ttacactcat cctacaaagt tagtttaaac tcttacaatt ctgtaagaat atactaaaac
    29341 aacatccact tcctagttta gattctacta taatgtttcc attttctttt tctaaaatta
    29401 atcttgtaag atatagacca atccctgtgc cattttggtt ttccacattt ttacttctgt
    29461 aaaatctttt aaaaatatca tttagttctc tactatcaat acctattcct tcatctttaa
    29521 tagtaatctt cgtatatagc tccattttta ttacagaaat agtaatcgtg ctatctgatg
    29581 gtgagtattt tattgcattt tctaaaacat ttacaattgc ttctatggtc cactttttat
    29641 tatgaactaa ctttatatct gaaaatggtt cgagatttat ccttatattt ttagcttctg
    29701 atttattaaa gatagtactg atgctttgaa taagagtatc tttaattagt agttcttcta
    29761 cttcaaattc tataactcca tcctctaatt tagtcatttt aaataaggat tgtaacaacc
    29821 agtcaatctt aaaagtttca tttttcatct tttgtaaaaa ctccttcttc tgtaatgtgg
    29881 aaatatttat attctccaga agttcacaat aaatggttat atttgaaagt ggagttttta
    29941 attgatgtga catatttgat attaaacctt taatcgcttc tttttctgac tttgactgtt
    30001 ctacctctat ttctatcata tccttaattt tttttacttg gtgagaaatt tttgacgctc
    30061 ttgtatcctt cgtatctgga taagtaactt ctttaccttc caatatttga tttaacatca
    30121 aatttaattc atcatatctt ctttgattta ttttagtaga tactataaat ataacaactg
    30181 tacatattat aaaggctaat ataaacatat tattctacct cattccataa ataacccatt
    30241 ccataagctg tttttatata ttttggatta gatgggtcat cctctacttt tactctaagt
    30301 cttcttatat ttacagatac aatattatca tcaacataat tttcttcgct gtcccaaata
    30361 tggtgtaaaa tttgttcctt taataatatc tgacctttat tttcaattag ataacttaga
    30421 agcttatatt ctacagagct aagttccaaa catgctttat ttttatatac ttttagtaac
    30481 ttttggtcca attttattcc attggaatat attactttat tattcgattt ttttcttatt
    30541 gctactttga ctcttgcctt taatacagat aaagaaaatg gttttgtaat ataatcatca
    30601 agccctattt ctaatccttt tacttcatca atttctgagt ctcttgcagt aagcattata
    30661 attggtatat ctgacttttc ccttattatt tgacatacat caaatccatc tccatctgga
    30721 agattgcagt ctaagattat aaaatctata tttttactat taaatatatt taatccttct
    30781 tgttttgtat aagccttaaa tacattaaat ccatcattct caaatgcata aaaaagacct
    30841 tcatttaagt ttatatcatc ttcaataatt aataagttca attttactct cctttagatt
    30901 ttttagataa ataattattt tcttgcatta tcatactaaa aatattttaa ttttatttat
    30961 ttcactaaaa agattgtatt taaatttgtt ttcaaaataa aaacatcata atcaagcata
    31021 tagttatata tttcccaatt taattacgct taaattgaaa atataacgtt attcttatat
    31081 aattaaatta tacaacaata aagttatatt ttttaaatat atttttacta gggggaatag
    31141 aattgaaaag ggttgatgag agaaatacta tgtttgcaag agcaaattac aaaaaagata
    31201 gtactgctta taatgattat tataagaaaa atcctgacaa aaaagaaatt gatgatagta
    31261 taagaaatag accaaattta tgtagtgaag gaacaatgac ttacaatgag ttaaactctc
    31321 caatggcaag tagtgcattc gattttttat ctgatatcaa atctttatgc gaaggaaaag
    31381 tttctgatac taaagtagat gtagatgcaa agtcaatgac aaaaaaaatt aagggtcttg
    31441 caaagcagta tggagcttct gtagttggta taactaaatt aaaagactat catatttata
    31501 caaatagggg tagacatgaa gaaaattacg gtgaagaaat taatctaact cataagtatg
    31561 caattgtatt tggatgtgag atggataaag aaatgataaa tagagctcca atgatatgtg
    31621 aagttataga aacatcaaag tgctatgtgg atgcatctat cgtcggcatg attttaagtt
    31681 actatataag aaatttagga tatgatgcta gaaatcatat ggatgctaat tatttagtaa
    31741 tgcctgtttt tatagctcgt gatgcaggtt taggagatat aggaagaaat gctatactta
    31801 caaataaaga ttatggctct agacttagat taggtgttgt tactacagat attcctcttt
    31861 tagaagatga atatgttgat tttggactag aagatttttg taaggtctgt aaaaaatgct
    31921 cttttaactg cccttctcat tcgctttcta atgatattaa aattggtgat gatggaaaat
    31981 ataattgggt aattgaacat gagacatgct atattaaatg gagatattta ggtacagatt
    32041 gtggtatgtg tatatctgct tgcccattta gccaaaatct cgaaactata aagaatacta
    32101 cttcatttaa gggtaataat gagcttattc aaaaagcttt agatgagtat acttcaaaat
    32161 ttggtaaaag aatatttata cctggaaatc catcttggtt gaaataattt tatttaataa
    32221 agaatctgta ctagaattta tcataggaca ggttctttat taaatatata aatttttaaa
    32281 tactttttct cgtttataac aattcagcaa tgtttttagt ctataattga cattataaaa
    32341 tattaaacta aaaggagtaa ctgtatggaa aaaatattag tagtagaaga tgattctata
    32401 ttaaataaaa ctctatcata taacctaatt gaagatggat atataattac ttcaaaattt
    32461 acagctaaat ctgcactaaa gtctattttt gaatgtgaat ttgatttaat aattttggat
    32521 attaatttac cagataaaag tgggtttgaa ttatgtaatg aaataaagga aaattacaat
    32581 ataccaataa tttttttaac tgctaatgat atggaatgtg atatgattaa aggctatgaa
    32641 cttggagctt tggattatat aacaaaacca tttaatatca atttatttaa acagaaagtt
    32701 aaagcttttt taaatcatct tacaacaaaa acaaaacaag attactatag ggatggatat
    32761 cttgaaatta acttttctga gttatctgcc aatataaatg gaaatcaaat tatatttaca
    32821 ccattagaat atcgtacttt aaaactgctt actgaaaatc ctaaaagtat tttaacaagg
    32881 aaagtacttt tagaaaaact ctgggatata gatgctaact ttgtagatga acatacttta
    32941 acatctgtta taagtaggat tagaagcaaa attgaaaaag acaatcttaa gtatatcaaa
    33001 acggtatatg gtatgggata tatgtggtta ggtgaaagaa atgaatttta aaaaattttc
    33061 ttttagttct atttatttaa taattgctgt tggtataatt tttacagcta ttttatttaa
    33121 tatttcaatt tatctaataa ctgatgattt aaaactaatt gctctattat taatatatac
    33181 aatagtactt tgtggattta tagcattaat aatattttta cttcataaaa aaatagttat
    33241 attttgttct aaaatatgtc acactttaga tagtatgatg aatagtgaag acaatattga
    33301 aattgaatta gaagaagaaa ctattttatc acgtatcaat cacagattaa ttagaatgta
    33361 tgaagttatt caagaaaatc gcaatagtat tgctatagag aaagctgatt tacaagaact
    33421 agtttctgat ctatctcatc aaattaaaac tcctattgca aatttaaaaa tgattaatac
    33481 tactctacta aatcaaacat ttaaaacaga aattcaaaag gattttttaa tggatatgga
    33541 aaaccaatta gataaattag attttttaat gcaatctatg gtaaaaacct ccagactaga
    33601 gacaggtata atcacactat ctaaaaagaa aaattcaatt tatgaaacaa ttgctacagc
    33661 acttagtgga attttgtttg atgctgaaaa aaagaatatt gaagttacag ttgattgtga
    33721 cccaaattta tatataattc atgacaagaa atggacttct gaagctattt ttaatatatt
    33781 agataatgct gtaaaataca caaattctaa tggtaagata cgtgtagcta cagaatgttg
    33841 ggttatgtat acaaaaatag atattattga taatggaaaa ggaatatcag aaagtcatca
    33901 agcagaaatc tttaaaagat tttatcgtga agaagatgta catgacatag aaggtattgg
    33961 aataggtcta tatctttcaa gaaaaattat atctcttcaa ggtggctata ttaaagttac
    34021 atcagatatt ggaaaagggt cgactttttc tatcttctta cctaataaat aaatttttaa
    34081 atattttatt tgtaatgatt ctgcaacatt ttgattttat actgtattta caagatgtta
    34141 aggaggtatt attatgagta ttttacaagt aattaattta aagaaatatt atggacaaag
    34201 ttcaaacatt acaaaagctc ttgatggtgt aactctatct attgaaaaag gcgaatttat
    34261 ttctattgta ggtacatctg gtagtggcaa atccacatta ttaaatatga tgggaggatt
    34321 agataatcct acatctggaa aggttattat aaagggtaag gaattatcta ctatgactga
    34381 tgaacagctt acaatcttta gacgcagaaa tataggtttt atatttcaaa attataatct
    34441 tgttccttta cttaatgtat atgaaaatat tatcttacca ataggattgg atggagagac
    34501 catagataag gattttataa atgaaattat aaatatgctt tctcttgaag aaaaattata
    34561 tgatatgcca agtaatcttt ctggtggtca acagcaacgt gttgctatag ctagagcact
    34621 tgtaacaaaa ccagctatta tactagctga tgaacccaca ggtagccttg atagtagaac
    34681 tagttcagat gtactgagtt tacttaaagt gacaagtaaa aaattcaacc aaacaattgt
    34741 aatgattaca cacaataatg aaattgcaca actaactgat agaattattc gtattgaaga
    34801 tggaaaaatt gttaactagg aggtgtagta tatgtttaga aataataatc gcacatttat
    34861 aagaaaaata gctttaaatg atttaaaaat taataaactt aaaacatatc tttcaggtat
    34921 cattataatg atttctacat gtttattatt aacagtgacc ttagtttcat ataatgcttc
    34981 tgtagattta gttaatgcat ctccatatca tgctatatat aaatctgtag atgaaaaagc
    35041 aaaaaatata ctatataaag ataaaaacct tagtggcgta ggaacctaca aattaattgg
    35101 aagtaataaa aagacagact gtattatgtc gattgtttat gcagatgata cagcaattaa
    35161 acttatgaat tttcaaccat tgaaaggcaa actacctaca aataaaaatg aaattgctat
    35221 ttcagaaaaa tatttacagg aatttggttt agataaagat attggagata gcattaaatt
    35281 aaattattat gatgatatta cgaataaaga agttcaatgt gattttatta ttgttggatt
    35341 tctagaaaat tattatcaag ataatgccaa gcaatactat acagtcgttt caaatgatta
    35401 ttttaagaat atagcttcta cacctttaca aaataaaagt aatacttttg acgagtcaag
    35461 acctgatact gtagatgtat tagttaaact atcagaagaa aatacagaga aagattcagc
    35521 ttcaattaag acacagttaa agaaaattgg tttatcttta ggtattaaga aatataatat
    35581 atacttaaat gataactata ttgaatcaaa tttaattgat ggtgagcaga ttattacagt
    35641 tttagtagtt ggaatagttg ttcttttttc tagcgttttt gttatatata gtatttttta
    35701 tatttcagtt gttaattcag ttcagatgta tgctaagttg aaatcgttag gtatgacttc
    35761 ttttcagtta aaaaagacta tttctctaca aggaaatatt ttgtcaatta tttttattcc
    35821 attaggggct atagcttctt gtataattgc ttacataatt caaccattag catggcaaat
    35881 gatagctgat ttatttataa ttttaatttt atcactggta atgtttttaa ctgttagaat
    35941 ttctttattt aaaccagcta gaattatttc taaaatatct gctattgaag ccatgcaata
    36001 tacagaaact aaatttagaa aacaagcaaa aaattttagt tatataaata taaaaaatct
    36061 agcattaaag aatatcgagt caaatagaaa gaaaaatttg attgcattaa tatcgctaag
    36121 cattagtgga gttttattta tttcaatcgc taatcttgct aattcaatag atttcaaaaa
    36181 acaactatct caacaatttg tctataacga agattatata attagcatta agagtgataa
    36241 cttatatgag catattacag aaatccaaca aaataatcca ctaacagatt ctcttaagaa
    36301 tgaaattaaa tctattcatg gagtaaaaaa attaattgaa tccaaatcaa ttaaaagtaa
    36361 tattatagaa ccttacatca aagatagaga cgaaaaacaa tttattactc taattaaagg
    36421 tattacgcct gaactttcaa aatcacttga agaatatatt gtcagtggaa aaatagacta
    36481 tacacattta gattcagata gtttaattat aaataaatat agaacaaaat cttatggatt
    36541 aaatttaaaa gttggagata cagttacatt taatatcttt agtggaaatg atattattaa
    36601 aaagaaaatg aaaattatag gaatcattga caattccaat acaggaggta tgttttttgc
    36661 ttcagacaaa actttagaat ctctaacacc atataataca aatttagatt tttctatttt
    36721 aatagataaa aagcattcta aagaagttga agaaaagttg ttaagtataa ttcagaaaaa
    36781 ttcaaattta atgctttatt catatgaaga tgattataga atgattacta gagcatttca
    36841 atatataatt atagcttcat acatttttgt tggtttaatt tcttgctttg gtatactaaa
    36901 tatgaccaat accctaataa acagcgtttt aattagaaaa aaagagtttg ccttacttca
    36961 agccgttgga atgacaagaa agcaactgcg aaatatgctt tatagagaag gtctaaacat
    37021 aagtatcaaa gctatatgta cttcaagtat actaggttat tttggttcaa atctactttg
    37081 cacttttata aaagatgtaa ttagattaga ctttattaat tttaaatttt ctatatttac
    37141 tattttaatt ttttcattcg tattaattgg tatacaagtt ttagtgactg aattacttat
    37201 aagaaatata gaaaaaaaat cacttacaga acgcttacgt tctcagtaag aatgtatata
    37261 aaaaataacc attttttata caagttttaa cataattttt taaaagctaa aaggatagaa
    37321 aattttgact ttacataatt ttctgtccct ctacttgttt ttattttata cagatacact
    37381 tccttatatc tccaagcaac atcaccagag acataaacaa gcatattcct tattttctaa
    37441 atatgtcaat tgcatgactt gtaactccta ctaaatcttc acgttcacaa gtagcaaggt
    37501 gatattttaa atataacgca aatgcatcat catccataga gtctactgct tcacgcatat
    37561 acaaagccaa accatctgat gcgacataat gcagtctagt tacattaaat gcagacatca
    37621 aatcatcaat gttttcttta cgtacaagct caaacaaatc ctttggttca gacttagcag
    37681 caaatgtctg tggatcaatc aaaccttttt caatatattc agatacatta atatttcctc
    37741 tatggaaccc ctcgtcaatg agacagccat cagatataac gtatgcagca aaaaccacac
    37801 caccaggttt tgttacacga attgcctcat gtaaagcttg ttgtttatct tctttgttat
    37861 aaagatgata tagtggtcct aacagtaacg taatatcata tttattatct gggaaaacag
    37921 ataaatccat agcattaccc tgagtaatgc ttatattttc atttgattga gtatttttat
    37981 ggaaaacctc tatattatgt tcgactaatt ccactgcgtc aacatcatat ccctgacgtg
    38041 ccagtgtgtg agaataacga ccagttgcag cgccaatttc aagcacacaa tcacctgatt
    38101 ttatgtactt atcaatgtaa tgcatagtag tgagaaattc caccattcca tgttttaatg
    38161 caagtcgact atcttcatca taagtattat aaaagtcaat taagtattgt tttgtttcca
    38221 taatctcacc tcttctatat tagttaataa acatcaaata gacaaattcc aatctggttt
    38281 ttcttgtctt ataaaagctg ttaatatatt tttcaacatt ttttcagcac tatatctatc
    38341 taatgttaat ttttttgttg ctgttaagac agctatgcca tgagtataaa gaaataaatc
    38401 taaaaatatt acttttgcac attccaactc tattccaatg ctttccgaaa aaagtcttgc
    38461 ctttttttca ttgtcaattt ctttataaaa atcattcgcc tctttcatca tcaaatctaa
    38521 atcatttata aataacaact taaataaata cgtttcttct tgagcaaatt caatgtatga
    38581 aagcggaagc agtaaataag ggcttacact tttagaatta cgataattaa caacatactg
    38641 attataatat tcatatgcaa aagcaagaaa ttcatttttt aattcattca tgttttcgta
    38701 acaagtaaaa attggtcgag tggaacactg taatttactt gcaatactcc tcgcatttac
    38761 agcttcaaat cccacttctc ttgtaatatc caagactgta ttcaaaatca tttcttttgt
    38821 tatctttact ttaggtggca tatatgttca cctctccatt aaatttttat aaaacacata
    38881 tgttatataa ctatagtaac atatcaagaa attatgtcaa gaaaaagctt tgcatttttt
    38941 tgcaattcct aaaacttata aataaaacat gagttgcaat taatctatta atagataaca
    39001 gcaactcata ttttccttta aatactcttc tttttgtcta atatttcata tacacaatct
    39061 tctattgaat taatatcttt ttcttttctt tttttagaat caattaaaat gaaatacagt
    39121 ataaaaccta cagcaagtcc ccatgttccg cctttagcag caattacacc tcccataagt
    39181 ccaatcaatc ccttctcaac acctgtatta catagctcca ttgatagctt tgtacataaa
    39241 tatccttgaa ctaaaagtgt tactgacaat gctaatggta taagaggtgt agcaatctgc
    39301 gcaattggat aaaaacagat aaaaattgct gaaacccata ctaatgtacc catgccacta
    39361 aaaacagact gcatggcttc ctttccaacc ttgtaccttt gagcaacact tcctgtaagt
    39421 gtcgcagcta atggaccgca cattggtata tatggagaaa agattgccat aaaaatattt
    39481 cttatcccac ttataacatt agacctattt gaattgaaat ctatatattc atcttttcta
    39541 ttttcacttt cagaaagtac taagttttca cctgttataa aatcaccaaa ggcaattatg
    39601 tatatcataa aagccattgg tatcccttgt ataaataaca ttacacttgg aaaacctatt
    39661 ccaaaaggag atactgtatt tatcagattt ttaaaatcta ctatgtaaac taaattgtca
    39721 aaactaaatc taggaatttc tatttcttta ctaaatatac ctactattaa actcactaaa
    39781 atagctgata ctacaccata ttctcctaaa tgaaataaaa atttattctt aacttttaga
    39841 gtatcaaatc tttcagaaaa cattacaaaa caagcaacca aaattcctac tgttatagat
    39901 ataggataag aatcaaatct ccctttagga ttaaactctg atattatagt cgatataccg
    39961 acacccatta gtattccacc tttaatagaa tttggtatct tactaactaa ctttccagct
    40021 acaccagtta tacccattaa aataaatatt attcctaaat ctatttgtaa agctgttaat
    40081 gcttgtattc tttcaatgcc catgctgaac tttaacaaat atgcagttgt aagagttata
    40141 gaagctgtaa tccagccagg tacaactggg tctcccataa aaacatgcaa acagtataat
    40201 attgagttaa taatagccat actccatgcc acttcatagg gtaatcccaa aatttgtgta
    40261 ctaaggccaa ttgtgcctaa acttgtcata cttgatatta taccttgaat agcttctggt
    40321 ttctcaaatc tgtaatgtat aaatggaatt cttatcttaa acattccaaa ttttatatga
    40381 ggttgttccc ttctagcgag tctttccata acttcactcc acctttcaat aactcttata
    40441 agtttacaac tttataattg tttataaatt gtcaatgtca ttaaaattag tatataacat
    40501 gcaaattata taaacaataa attaaatcta taaaattgat agattttgtg catatataga
    40561 aaaaccgctt catttttagt tacttaaatc acttaatatt agttatttaa atcacttgat
    40621 aaaagttttt tataaaattc aatagttcta ttccatgatt caaactgtcc ttttgaattt
    40681 ccttctacag ttccacctga agaaaaaatc cctccactca tactcataca taaagtctcc
    40741 tttagaggca taatatatgg aattggtaac ggatgaccca tattattgta tgttaataat
    40801 tctacgttat atggataatt attttcttca aagatttcta atgcctctaa acaagcttca
    40861 taactattcc atatgttatc ttcttttcca gcaatcataa gtatatctgc tttggaattt
    40921 tctattttta ttctagcctc ttttctattc tctgctcttt taagactttt cttataagta
    40981 ctattaaatc caaatggaat accttttgat atattttttt tctgttcttc aaagaatata
    41041 tcattgtcta ctttgatata tggaaatggc tctcctttat atgaccatga tgaagtatca
    41101 tttccactca tcaatccatt aagagcttgg aaacaataaa tgtgaggttg tgatactaca
    41161 acctttttaa tttgttcata tcttgaagca agtaaaaagg ccaattctcc accctttgat
    41221 gtcccatgta caaatatttc tttagtactg gtaatttcat tttcactaat ccactcaaat
    41281 actttttcaa aatattctaa tggaacctct tctaatttat ctggtaaccc ttctaaagca
    41341 aagtatgcaa ctgttagtac attgaatcct ctagatgcaa gtggagctgc taaaaggtct
    41401 agagcgtcta aatttccatc agaaccacct agcattaaaa ttgttttatt attagaattt
    41461 tgtttataaa ataattttcc tataaactca tcagatatgt attttgtggt aatatcttct
    41521 gatttaaata ttctctttaa ttcaacttct tttctttcag tagaagtttc aaatatcata
    41581 ttcataatta ttggtttatc aatagatata ttttctgcaa aatttttacc ttcagtttta
    41641 gatttttcca gcgaataaat taaccccata caattatcag ccttataagt accttctata
    41701 ggttcaacta aatctaaatc tacttctcct ttttcatttg aacaaaacac tccataagat
    41761 gaaaattctt ctcccttgca ccatggaaat tccattttca cacttatttt aagtttagta
    41821 tttggaatta agtctgaaac cataatctta agtctttcat ccacaaatga ttctacattg
    41881 tatacattta ctttcataat ctatttctcc ttataataat tatttatgct ttatatataa
    41941 aacaacttta tctaatacaa cataaataaa tttttaaatt aagacaccat attgactatt
    42001 tcattttatc aacatatttc atttttgtca atatattgtc ttttttagta atttaaaatt
    42061 atgtagtcta tatgaaatcc agttgatttt tggctaatta aagataggaa tgctattagg
    42121 tttaaaatat atctattcaa aataaaaaac cactcatttt aagaatggtt tttaatataa
    42181 actatttgtt attaataagg atattttttt atttaaattt atataattga acatctaata
    42241 tttaatgttt aataaaatta tgcatttgca aaagtatata tggcattaac tcttgctaca
    42301 tataaattct catcactaca tccaatatat ctagcatatt tttcaatctt ttcttttgca
    42361 ttttcaacat cattttcatc gctctccatt aactcattta tataaatatc cattaatgac
    42421 gctttatatt cttttatatc atagcctaaa aattcagtca atctctcaat ttccttacta
    42481 aaatctccat cttcttttac caaaaattcg tatacatctt ccctatatgg atttaaagtt
    42541 attatttgtt ttactacttt gcattcgtca gccttcccaa taaaaccatc tttataatta
    42601 ttaaataagg cattagattt ttttatagat tcttcatcaa tataagaatg taccgcagtt
    42661 atacgatttt ctataagggc atctacaaca gcataatgaa ttttaaaaca atcattgtat
    42721 aaataattgc tcattttctt tattgtcgat tctatttcta agaagctagc gtgcccattt
    42781 tttttatttt tatttggtat aataaaagtt ttattcaagt tattaaaaag tctcttaaaa
    42841 tctaaatatt tttcacaata aacgctttta aaggtatcga tatctatagt tataattcca
    42901 aaacttacta tagtctcaat tcctttctta aaagactctt ctatatattt ttctcctaaa
    42961 cttaaacctc tatcagatat ttgctttaga ttttgaaaac tatttaaaca atctctctca
    43021 aagtttttag atatttctaa agcatataat ttaaagattt tccttatctt attataatta
    43081 ttttttgtat catcaaatct gataatagct ccatttacat caaaagtaat cattgagttt
    43141 taacctcccc attagtttca tcaataaaat aaatttaata taaactttat aatcataata
    43201 ttaaacttat ttatactata attattaatt tgatgataaa agctattttc cttctaaata
    43261 aaaaatttgt ctaaagtttc taaaaaattt agacaaatct taggttatta ttctttaaat
    43321 tatttttaaa ttattccaaa tacaccaaat aatattggta taaatatagc tggtaacaaa
    43381 ttagctactt ttatattcgt taatttaagt acatttagac caagtcctat aattaataaa
    43441 cttccagctg ctgtcatttc tgtgactaca ggatcactta aaaatccatt taaaaatgat
    43501 gctcccacac atataatacc ttcatacaaa aatacagata ttgcagaaaa cattacacct
    43561 atacctaaag aagcagtaaa tataattgaa gctattccat ctataacaga ttttacaaat
    43621 aaagtatcat gacttcctgt aagaccactt tctaatgaac caactactgc catggctcct
    43681 atacagaata ataaactaga tgatataaaa ccctctgcta tagaatcttt tttatttcct
    43741 ttagaaaatt tactttgtaa aaaagcaccc aacttattta cccatttgtc aatatctatt
    43801 aattctccta tcagagcacc tacagctacg gatattattg tcactaatgt gttttttcct
    43861 tctagtgctc ctgatatacc aatgtacaat acacataagg caattccatt cattatagta
    43921 tcacttactc tttgaggtaa tcctcctttt attataagac ctactataca tccacctgca
    43981 attgctagac tatttgctat tactccaaac attttttctc ccctcgatta gctaattgaa
    44041 atttatccta ttattaaaac ctaagcatct atactttgta ttggatattt taaatattcc
    44101 aaaaattctt caaaaccttt tctagatata tttacagttt tagtatttat gttaggatga
    44161 aaatttaagt aatcttcttt aattacatct ttatctatgt aaagctcaac ttttctttct
    44221 gtatcattta ttaatgaaaa aggatttaca cttccaggaa gaagtttaag tacattgtat
    44281 agtctttcag gtgaagcaaa cgataatcta gaacttccta tcttatcttt aagtgagttt
    44341 aaatcaactt gtttttctcc tttaactaca ataagataat acttatttcc tttggcattt
    44401 cttaaaaata aatttttaca ttgacacccc tttgttattc catctaaaat gactaattgc
    44461 tctgctgtat atacagcttc atgctctact acatcatatt caacatttaa tttatccaaa
    44521 atatcatata ttttagtttc attcatgcta gattcatttt catcttgttc ttttttatta
    44581 tttaaatcac tcatactttt ctccttaaac acttcatcat aatatcattg tgaggtatta
    44641 ttctactata gctattgctc ttactggagc tccatcagca tcattatact taaatggtgc
    44701 tgctataaat agaaattttt ctggaacatt ttctaaattt gtaagatttt ctatgattat
    44761 ttttcctttt tcaaataaaa tatgatgcac ttcaaatact gatgtgttat atctatccac
    44821 tgatagcata tctattccta tgccttttat attagtatta gctatatagt ttgcagcttc
    44881 ttttgttaat gtaggataac ctacatagta ctgcttttta tcccaaaatt ttgaccaacc
    44941 tgatttaaat attataaaat cactattttt tatttcatct tcatattttt ttagatattc
    45001 taattctata ttctcatttc ccttttcaag ttccaataca taagctttcc caacaaaatt
    45061 ttctatatcc aacttatcta gggtctcacc tttagtatac atatgtcttg gtgaatccat
    45121 atgagttcca ttgtgagaaa atacactaat taatgtttct tgataaccat tttcttcaat
    45181 aatagctact ttcttgatat ctggtctatt aggctctgca tatacaggca tgtcattatg
    45241 tgtcacatga gttaaatcaa aaaccttcat ctttatatac cccctatgtt tttattttct
    45301 attatatacc taaattcatt ttatttctat cagattttat tccattatat accatactac
    45361 tatatatttt ttataaacct caagggattt accgaaaaat cttggaaaga attatcagta
    45421 aatataacat cttcacccaa aaaacgttta taccttcctt cttctttctt taaagtaatt
    45481 ctctgttttt gtgaatgatt attaaaaacc attatcttaa gaggaggttt ttttctatta
    45541 gtctgcactc tttttaacat gtattttatg tgcatatcag cttcaggaaa tgaatatcca
    45601 caaaatatta aaaggtcagt atctcttaaa gttttttctg tttcattcca aacattactt
    45661 aaaaatatgt ttgacatatt cttaaaatat gttggtggca ctataatagg ttctgttaac
    45721 tcaccacact ctaaacattt tgtctctgaa ctattttcaa taagcttcat gacaccacct
    45781 tcatgtggtg taagagttac actattacaa atggggcaat aaagccaatt caatgaccca
    45841 tgtatcttat ataatttgat taatggttct tttggttttc tccaatttaa ctcctcttta
    45901 aaatttgtaa actccacacc ataatcaagc ataattggca aatcattctt atctcttaat
    45961 ccagtaactg tattgtctat atgtatatca taatttgcac taataaaagt agtatcaaga
    46021 agcaaatcat tttccaaaag attttcaact aaaagtttat gatatttatt atctgttcta
    46081 ggtgtattat gaattgcttc agccattagc agtattaaat attgtcttaa aaaacgtata
    46141 ctatcacttc tgttatttag attttctagt ccaaaatttc taaatgcttc ttttctttgt
    46201 tctgctaagt ctaaaagacc taaaacttct tcaaaggtag gaaaatttgc attatctata
    46261 ttatctttta ctatatcaat attgaacatc tgtttaaaga aaatatacaa ttctctattc
    46321 attttggtat tataatttgt gggatttagt tttttaaaat attcgctaaa aagttcattt
    46381 tgaataggta gattctctgc tgctgaagct cctgctccta aaaatattgc tattttcata
    46441 tagttcctcc taatataaat attactccta tttttatatc cattaaatat ccatatcatt
    46501 tattttatga tacaacaata aagctgtctt ttactagaaa aaatcctatt acaagacagc
    46561 cttatattat attaaatctt taataattcg taaatagaaa gagcatatat ttgagtagct
    46621 tttataatat cctctactat taagtattca ttagcttggt gttctgtttc tggttttcct
    46681 ggaaaaatag ctccaaatgc aacacaatta tctaaagctc ttgcgtatgt tgctcctcca
    46741 gatgaaagtg gtgttccatc aagaccagtt tcttcttcat atacttttct aagtgtctta
    46801 acaagaagag tatcttctgg aacatagatt gaatccaaga aatcatattc ttcataattt
    46861 aaattgtatt tatctgtcat tttctttaac tcttttacaa aatcatcctt tttgtatgtt
    46921 actggaatcc ttacatctat tcctgcaaat tctttttcgt tatcaatagt tactctacct
    46981 atatttactg ttagtttacc tgatacatca tccttgcaat taggaattat attgtttcca
    47041 ttagcatctt caccaataac ttctgctaaa aacttaatta tattagaatc tatacctata
    47101 ttattaagag ctatacaaag tctagccact gcatttatac cagtatcact tgctgctgaa
    47161 tggacacttt ttccaattat acagatttta ttaccttcaa cagtatactc aaaatttaat
    47221 ttatcaagtt ctttttttaa tttatcagag tattttccaa tatagattgc tttacctgga
    47281 actgcattta aagcctttcc aactgacaac tctatatcac ttttgctatc acaagtcaaa
    47341 tgaacttgta aaagaccttt ctctgcattt gttattggga atctagaatc tggagtaaat
    47401 ccatagtttg gtatttcttc attattttct ttatatttat ttatacatct ccaaagattt
    47461 tcttcatcag ccccaaaaat gaatcttatc ctcttattaa agtctacatt taaatccata
    47521 agagctttca ctgcataaat tgctgatatt gtaggtcctt tatcatcttg tgttcctctt
    47581 ccatacaatt taccatcttc taaaacagct tcaaatggag ggtaattcca gctttctaaa
    47641 tcaccttctg gaaccacatc aacgtgacca agtataccta tcattttttc accttgtcct
    47701 atttctgcat atccataata tccatctttg tatactgtct taaatcctaa agatttacat
    47761 aagtctaata tacctcttaa agctttatct acattttcac caaatgggca attttcagtt
    47821 gcttcagata taacactagg tatttttaca ctttcttgta ttgaagaaat catttcatct
    47881 tgtagagaat ttactttttc tttaatttgc tctttcatat tatcaccctt caattttcat
    47941 cataatatac taataaatat tttgaccttt ttaaaaaaaa atatgcttat tttttcagtt
    48001 tttttataaa ttcaatcgct tttaaagtcc ccatttttga atggtcaaat gtaagtccac
    48061 cttgaaaata aacattataa ggaggtctta ttggagcatc tgcacttaat tctatagaag
    48121 aaccttgtat aaatgctcct gctgccatta tgacttcact ttcgtatcca ggcatgtccc
    48181 aaggaacagg ttttacataa gaatcgactg gtgcagcttc ttgtatacct tcacaaaaac
    48241 ttattacttc atcagcatta ttaagtctaa tacattgaat aatatcactt cttaagtcat
    48301 catattttgg taatacatca tatcctaatt tttcaaaagc tcttgagcaa aagatagctc
    48361 ccattacagc ctgagatact atataaggcg ccaagaaaaa accctgaagt acatttctag
    48421 ttgttccaaa agtaagacca cattcttttc ctattccagg agaagtcatt ctataagata
    48481 taagctcaat caaatctttt ctaccagcta tatatccacc tgtcaatgca agcccaccac
    48541 caggattttt tattaaagag ccagccataa catctgctcc aacatcagta ggctctttgg
    48601 tatctaaaaa ctcaccataa caattatcaa ccattactat agcttctggt ttaacagact
    48661 ttattacttc tatagcttct tttatatcac taattgaaag tgattttctc caagaatatc
    48721 cttttgacct ttgtatcata acgagtttag ttctatcatt tattttgttt ttaattcctt
    48781 ctaaatctaa gtttccatcc tctaaaaaat ctacatcatc atatgtaaca ccatactctt
    48841 tcaatgaacc tttttcttct cttatgccta taactccctc taaagtatca tatggtctac
    48901 cagtaacaga taaaatttca tctcctggtc taactatacc ttgtatacat aaagtaagtg
    48961 catgtgtacc atttacaatt attggtctta caagagcatc ttctgtatta aatactttag
    49021 aataaatttc ctctattttt tcacgaccaa tatcattata tccatatcct gtagtccagt
    49081 taaagtgcat atcacttagt ttagattctt gcatagcttt taaaacttta tattgattat
    49141 attctcttat ttcttttatt tcttcaaact tatcttttat ttcttccatt atttcttgcg
    49201 atagtttaaa agtatcatca tctattccat agtagtcttt taataattct tttgtctcat
    49261 taagcatttt atcacctgtc tctttattat tttatttaaa catgtaaagg agactatttt
    49321 actaaaacaa ataaattata gtttataagt tagtataagt ctcctctcaa tcatcttaaa
    49381 ttaaattaat tatctatctg ttgttattat tattgttgtt ttggttttga tttgttagat
    49441 taatatactt tccaggttgt atagttgata cagcatgttt ataaatcatg ttttgttgtc
    49501 tattatcccc ttctattaat attatataac tatcaaaccc ctttacaagc cctttaactt
    49561 gcactccatt aactaaatat atagtaacag gtatcctttc tttccttgca ttatttaaaa
    49621 acaaatcttg taaatttaaa actgtatttt tcattagatt tgtaccctcc caagattaaa
    49681 ttgaacttct aaaatattcc taccttatat tctatataag ttatgacaaa ttttcaatat
    49741 taagttgtat acttaaaaat ttatatatta ttttatagaa tctttaatat ataagattat
    49801 ttcatttttt aattcgtcta tattttcata ttggtctaaa tcaaaccatc ttgctgtttt
    49861 atatctttta aaccatgtta tctgacgttt tgcatacctt ctagaatctc ttttaattat
    49921 ttcaatagct tcttcgtatg tatattctcc atccagatac ttcaatatct ctttataacc
    49981 tataccttgc attgatatca tatctttttt aaatcccata cttaaaagtt tttttacctc
    50041 ttcaaccaat ccatttttaa tcataatatc aactctcata tttattcttt gatacaaatg
    50101 ttctctatct ctatttagaa ctattattat tggttgatat tcttcattaa attttaaatc
    50161 acttgaaaaa tcattcattt tcttaccact tagacaaact tcaagagctc ttatgaccct
    50221 tttagtattg tttttatgga ttctattagc agattcttca tcaagttctt ttaacttatt
    50281 atgcatgtaa tctattccct tttcagcaag ctgtttttga agttcttctc ttaattcatt
    50341 attggcatca gattgtgcaa aatccatatt atatattaaa gagtttaaat aaagtcctgt
    50401 gcctccagtt actaaaacac attttccctt ttcatttatt tcatgtatat atttttttgc
    50461 tctttgttga aactctgaaa ctgaaaatgg atagtcaggc ttaacttcat ctataaggta
    50521 atgcttcact ccttgcattt cttcctctgt aacttttgca cttccaatat ccatatattc
    50581 atatatttgc atagaatcag ctgaaattat ctctgcatct atatcttttg ctaattttat
    50641 tgacaaatct gtttttccaa cagcagttgg acctgtaagt attataagtg gtattttttt
    50701 cataacattc ctttccatat attttaaata tccaaatatt ttacataatt cttttaaaca
    50761 ttttttctat ttctgtctta gatatttcga ccattatagg tcttccatga gggcatgtaa
    50821 atggattctc acacttttct agctgttcca ataaactttt tatttcaata tcgtatattt
    50881 tatcatttgc ttttatagca gacctacatg ccatagatgc aaatctctct ccttttaaat
    50941 cataattact tgtaatttct tctatattat caattatttg aagtataaat ttctcagttt
    51001 caggtactcc aaatatagta ggaacacatc ttaccattat atgattattg ccaaaaattt
    51061 ctagttcaaa accaaatttc ataaataact ccaagttatt ttcaatttga agcatatcta
    51121 catttgatac ttcaataacc actgggtcta aaagtatttg catattgata tcttgtctat
    51181 agaacttctc catatatctt tcatatagta ttctttcatg tgcagcatgt tggtccaaca
    51241 aatacataga atcgtctttg ctcaaaatta tataagtatt aaatacaact ccaattacag
    51301 aatatccata taaggaaaac ttgcgttttg aactacacaa gtattcttct tcactgtcct
    51361 taatactatc tcctaagtaa taatttcctt catttttaat tccatcaact tgaaactctt
    51421 cttgtatatt atcatttgca ctatcttcac ttagataatt aatgtttttt acatcatctt
    51481 ctacacttgc atttaggact tcacttactg attgaaattc atttattggt ttttcacttg
    51541 ataaagtcac aacttcaatg acttcatcct tatctttagt aatattacta tttttaggag
    51601 tagattctaa taaatcatta tttcttaatt tataatctgt acttttttct ctactgttaa
    51661 tagcgattct aggttgtgtt tttttatcac tataagtagc atattttcca ataagatttg
    51721 agtgtattaa tttaaccttt aaaaaatctc taagttctat atacacttct tgttcttttt
    51781 caaatttaat ttcaagttta ttggggtgta tatttacatc tatacatgat gggtctactt
    51841 ctatatttaa gaagcacact gcatgtttac ctattggaat tatagactta taactttctg
    51901 ttattgcatc tatgattatt ttacttttta caaatctttt atttatatag atatgttgta
    51961 agtttttatt tgaacggtat atattgttat tacctatata tccattcatt ttaaagtgat
    52021 tgcacttaaa ctctacatct attatatttt ccgttatttc ttttccatat atacttctaa
    52081 tagtgtttac aagttttcca tcccctggag tatttaacat ctgtttattg ttatttgtat
    52141 atttaaattg aatgtttgga ttacctattg caagtttatt tatcaaatca cttatgttta
    52201 tagtttctgc atgagtagat tttaaaaatt tctgtcttgc tggtgtatta aagaaaatat
    52261 cttttataat gatggtagtt ccattagtag aacctattgg ttcttttgat attatttttc
    52321 cgccttcaac atatatttta gttcctatta tttcatcttt agttttagtc gtcatttcta
    52381 attttgatac agctgaaata gatgccaatg cttctcctct aaaaccaaga gagtataaat
    52441 catacaaatc atcaattttt ttaattttac tagtagcatg tctcaaaaat gatttttcaa
    52501 cttcacttga aggtattcct attccattat ctgtaatctt aattagactt tttcctccat
    52561 caattatatc tatggaaatt ttatttgcac ctgcatcaat ggagttttca ataagttctt
    52621 ttactacaga agaaggtctt tctacaactt cacctgctgc tatcttattt atagtcaaat
    52681 catctaggat gttaattata tttttcattt ttacacctct aagattttat ttcttttgct
    52741 tttctttgaa gattataaag agagtttata gcatctaatg gagtcatatt taaaatatcc
    52801 agatttaaaa tttcttctga tagtatatct ctatttacac tatcaaaaga gatttgagtt
    52861 aaggctactt cttttacaga atcttcttga ttaattttct ctttatcatc attttgcttc
    52921 attaaagcaa cttcatcatt taatgcttta aatttagaat ttaatttatc ataatccttt
    52981 tttatatgtt tgtagtcttt tactataagt tcatgttcta tttttagaga ttcatattta
    53041 ttcttaaaat tactttgatt taccttaact aattcttcgt ccaaattact attaattata
    53101 ttattgtttt ctttatctcc atttattgca acactgttgt aaacatgatt tttttccaag
    53161 tctttaagta tatattttgc tctttctatg acttcgtctg gtaatttagc taatttagca
    53221 acatatattc cataactctt gtctgctccc tgaggaatta tttttcttaa aaatattatt
    53281 ccttccccat cttctttgac tgctattgaa taatttttaa cctctttaaa ctcttcttct
    53341 aaatcagtaa gttcatgata atgtgttgca aataatgtct tacatctaat atttttttga
    53401 atatattcca ctattgacca tgctaaactt atcccatcat aggtactagt tccacgacct
    53461 atttcatcta atataactaa acttctctct gttgcatttt ttaatataag ggatacttca
    53521 ttcatctcta ccataaaagt agattgacct tgagataaat cgtcactagc tccaactctt
    53581 gtaaatattc tatctaatat tggaatatca gcagactctg ctggtacaaa tgagcctata
    53641 tgtgccataa gagctattat agcagtttgt ctcatatatg ttgatttacc tgacatatta
    53701 ggaccagtta ttatgttaat aatgttttct ccacgattta aataagtatc atttggaacg
    53761 aaattctcct ctcctactat attttcgaca acaggatgtc taccatttct tatatcaagt
    53821 ttattatttt catttattgc tggttttaca tagttattta tatgtgcaac tgttgcaagg
    53881 gacacaaaaa catctatatt agctatagtc ttagccactt tttgtattct atctatgttt
    53941 ttatatattg tatctcttat ctctacaaat atctcgtatt ctaaagattt aatcttttcc
    54001 tctgcatgta gtatcttttc ttctatttcc tttaattctg gagttatata tctttctgca
    54061 ttactaagtg tttgctttct tatatatgtc tcatctagct ttgcctgttt aaagttggct
    54121 tttgttattt ctatataata tccaaaaact ttattaaatc ctatttttaa tgatttaact
    54181 cctgtttttt ctctttccct gttttctatt tcctttacaa gaaaggctcc attcttagaa
    54241 atctctctta gttccttaag ttcatcacta aaatctgatt taattatgtt tccatctttt
    54301 atggttatcg taggttcttc tagaatagct tcatctatta aattgtatat atcatctaat
    54361 ttatccattt ctgaaacata ctcttttagg atttttgcat ttgataaatt tatagtatct
    54421 tttaaattag gtaacttttc tatcgaattt tttaagtgaa ttaactcttt tggtgttact
    54481 ctctcaaatg ctatctttcc acatattctt tctatatcgt atatattttt aagtatatca
    54541 tttaggtctt ctcttaatat aaagtcatct tttatttctt ctattacatc taatctattt
    54601 tcaattttag atttatttat caaaggttct tctacatatt ttcttaaaag tctacctccc
    54661 attgctgtag aagttttatc aagtacatgt aataatgaac cttttttctt attccctctt
    54721 atagtttgag taagttctaa attagttcta gtaaacatat ctaaaaccat atattctgat
    54781 gaattg
//
LOCUS       DAGQWR010000005        54321 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.005, whole genome shotgun sequence.
ACCESSION   DAGQWR010000005 DAGQWR010000000
VERSION     DAGQWR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54321
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.005"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            279..1118
                     /locus_tag="MIW95_000216"
     CDS             279..1118
                     /locus_tag="MIW95_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBY3149576.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            1115..2296
                     /locus_tag="MIW95_000217"
     CDS             1115..2296
                     /locus_tag="MIW95_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149577.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNIDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            2600..3502
                     /gene="ptb"
                     /locus_tag="MIW95_000218"
     CDS             2600..3502
                     /gene="ptb"
                     /locus_tag="MIW95_000218"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBY3149578.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            3524..4603
                     /gene="buk"
                     /locus_tag="MIW95_000219"
     CDS             3524..4603
                     /gene="buk"
                     /locus_tag="MIW95_000219"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBY3149579.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            4593..5270
                     /locus_tag="MIW95_000220"
     CDS             4593..5270
                     /locus_tag="MIW95_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY3149580.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            5313..5528
                     /locus_tag="MIW95_000221"
     CDS             5313..5528
                     /locus_tag="MIW95_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY3149581.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            5557..6636
                     /locus_tag="MIW95_000222"
     CDS             5557..6636
                     /locus_tag="MIW95_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBY3149582.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            6636..7388
                     /locus_tag="MIW95_000223"
     CDS             6636..7388
                     /locus_tag="MIW95_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBY3149583.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            7389..7946
                     /locus_tag="MIW95_000224"
     CDS             7389..7946
                     /locus_tag="MIW95_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBY3149584.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            8116..9462
                     /locus_tag="MIW95_000225"
     CDS             8116..9462
                     /locus_tag="MIW95_000225"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBY3149585.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILNGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            9729..11561
                     /gene="glmS"
                     /locus_tag="MIW95_000226"
     CDS             9729..11561
                     /gene="glmS"
                     /locus_tag="MIW95_000226"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBY3149586.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEEEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            12014..13309
                     /locus_tag="MIW95_000227"
     CDS             12014..13309
                     /locus_tag="MIW95_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBY3149587.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKMQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKRAVDFSANWLNVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSITPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSNSVSLEEYRNIDNTMRNKSEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            13786..14523
                     /locus_tag="MIW95_000228"
     CDS             13786..14523
                     /locus_tag="MIW95_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBY3149588.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            14544..15797
                     /gene="murA"
                     /locus_tag="MIW95_000229"
     CDS             14544..15797
                     /gene="murA"
                     /locus_tag="MIW95_000229"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBY3149589.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            15884..16948
                     /gene="spoIID"
                     /locus_tag="MIW95_000230"
     CDS             15884..16948
                     /gene="spoIID"
                     /locus_tag="MIW95_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBY3149590.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKSTVSYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            17014..17682
                     /locus_tag="MIW95_000231"
     CDS             17014..17682
                     /locus_tag="MIW95_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBY3149591.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            17780..18040
                     /gene="spoIIID"
                     /locus_tag="MIW95_000232"
     CDS             17780..18040
                     /gene="spoIIID"
                     /locus_tag="MIW95_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBY3149592.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            18237..19256
                     /locus_tag="MIW95_000233"
     CDS             18237..19256
                     /locus_tag="MIW95_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBY3149593.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            19272..19700
                     /gene="fabZ"
                     /locus_tag="MIW95_000234"
     CDS             19272..19700
                     /gene="fabZ"
                     /locus_tag="MIW95_000234"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBY3149594.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEIEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(19811..20320)
                     /gene="yyaC"
                     /locus_tag="MIW95_000235"
     CDS             complement(19811..20320)
                     /gene="yyaC"
                     /locus_tag="MIW95_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBY3149595.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      20509..20612
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            20749..21942
                     /locus_tag="MIW95_000236"
     CDS             20749..21942
                     /locus_tag="MIW95_000236"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBY3149596.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            22258..23355
                     /locus_tag="MIW95_000237"
     CDS             22258..23355
                     /locus_tag="MIW95_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149597.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSNIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            23619..25838
                     /locus_tag="MIW95_000238"
     CDS             23619..25838
                     /locus_tag="MIW95_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBY3149598.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            25841..26623
                     /locus_tag="MIW95_000239"
     CDS             25841..26623
                     /locus_tag="MIW95_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBY3149599.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            26955..28868
                     /locus_tag="MIW95_000240"
     CDS             26955..28868
                     /locus_tag="MIW95_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY3149600.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            29009..29317
                     /locus_tag="MIW95_000241"
     CDS             29009..29317
                     /locus_tag="MIW95_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY3149601.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            29342..29668
                     /locus_tag="MIW95_000242"
     CDS             29342..29668
                     /locus_tag="MIW95_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3149602.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            29707..31041
                     /gene="celB"
                     /locus_tag="MIW95_000243"
     CDS             29707..31041
                     /gene="celB"
                     /locus_tag="MIW95_000243"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBY3149603.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIVTPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            31052..31570
                     /locus_tag="MIW95_000244"
     CDS             31052..31570
                     /locus_tag="MIW95_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBY3149604.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNEKEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            31666..32625
                     /locus_tag="MIW95_000245"
     CDS             31666..32625
                     /locus_tag="MIW95_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBY3149605.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIVDFTFSVV
                     TKEESPAVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            32673..33725
                     /locus_tag="MIW95_000246"
     CDS             32673..33725
                     /locus_tag="MIW95_000246"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBY3149606.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISKKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKTPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSNTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            33728..34402
                     /locus_tag="MIW95_000247"
     CDS             33728..34402
                     /locus_tag="MIW95_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBY3149607.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            34955..35506
                     /gene="raiA"
                     /locus_tag="MIW95_000248"
     CDS             34955..35506
                     /gene="raiA"
                     /locus_tag="MIW95_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBY3149608.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDSEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            35638..38313
                     /gene="secA"
                     /locus_tag="MIW95_000249"
     CDS             35638..38313
                     /gene="secA"
                     /locus_tag="MIW95_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBY3149609.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMVREDLPDSVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            38328..39435
                     /gene="prfB"
                     /locus_tag="MIW95_000250"
     CDS             join(38328..38402,38404..39435)
                     /gene="prfB"
                     /locus_tag="MIW95_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBY3149610.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            39579..41720
                     /locus_tag="MIW95_000251"
     CDS             39579..41720
                     /locus_tag="MIW95_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBY3149611.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYNEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            41868..43034
                     /locus_tag="MIW95_000252"
     CDS             41868..43034
                     /locus_tag="MIW95_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY3149612.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      43094..43211
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            43336..43941
                     /locus_tag="MIW95_000253"
     CDS             43336..43941
                     /locus_tag="MIW95_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBY3149613.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     gene            44270..45310
                     /locus_tag="MIW95_000254"
     CDS             44270..45310
                     /locus_tag="MIW95_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBY3149614.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWEDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            45449..45901
                     /gene="tsaE"
                     /locus_tag="MIW95_000255"
     CDS             45449..45901
                     /gene="tsaE"
                     /locus_tag="MIW95_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBY3149615.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            45901..46617
                     /gene="tsaB"
                     /locus_tag="MIW95_000256"
     CDS             45901..46617
                     /gene="tsaB"
                     /locus_tag="MIW95_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBY3149616.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            46607..47083
                     /gene="rimI"
                     /locus_tag="MIW95_000257"
     CDS             46607..47083
                     /gene="rimI"
                     /locus_tag="MIW95_000257"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBY3149617.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKCIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            47089..48105
                     /gene="tsaD"
                     /locus_tag="MIW95_000258"
     CDS             47089..48105
                     /gene="tsaD"
                     /locus_tag="MIW95_000258"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBY3149618.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSNKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            48402..51110
                     /gene="hpdB"
                     /locus_tag="MIW95_000259"
     CDS             48402..51110
                     /gene="hpdB"
                     /locus_tag="MIW95_000259"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBY3149619.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            51114..51371
                     /gene="hpdC"
                     /locus_tag="MIW95_000260"
     CDS             51114..51371
                     /gene="hpdC"
                     /locus_tag="MIW95_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBY3149620.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDSACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            51364..52314
                     /gene="hpdA"
                     /locus_tag="MIW95_000261"
     CDS             51364..52314
                     /gene="hpdA"
                     /locus_tag="MIW95_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBY3149621.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFKDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSAENISDIISFMHKNNLIEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDIDEEHLEELQDIFLDNGIACYVGHETAF"
     gene            52634..53596
                     /locus_tag="MIW95_000262"
     CDS             52634..53596
                     /locus_tag="MIW95_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY3149622.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIITVMAAFYIFEYYLSTILMEI
                     IRKILIATGDINLKTNYFSEYANWLNDVGLQVVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(53695..>54321)
                     /locus_tag="MIW95_000263"
     CDS             complement(53695..>54321)
                     /locus_tag="MIW95_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY3149623.1"
                     /translation="ILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIVPT
                     SFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLGV
                     IILGMCWSLWHLPLIFTLYTPEAPILGVILRSIHIVGISIFLGYLYIKTKNIWFCAII
                     HVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT        21882 a         5777 c        10296 g        16366 t
ORIGIN      
        1 attaagatat tttagaacat aaaactgcat taaggcgcga gtactatttt gtattcgcgc
       61 cttgatggtt tgagttattt gagtaaaaac taactttaat aagaaatatg tatttattaa
      121 gttgatattt ttaatgtgtt tagagattat ttaacaatct ccaaatataa aactagaatt
      181 atttacataa aagttaaata gtttgtatag agttagattt atatagtata atacttagtg
      241 taaataatta agaagttatc aaacaggagg ataaaactgt gttagatata aattcttttt
      301 taaatggatt ttttaatata atattaagaa tgagtatata tgatttaatt gacatatcta
      361 tagtagcgta tattttttac aaaatattta tgtttataaa agatacaaga gcagaacaag
      421 tgtttaaagg tataatattt cttttattag caactcaact tagtaacacc tttaaattgc
      481 ataccgttta ttggatatct cttaaagctt tagattatgg agttatagct gctcttataa
      541 tttttcaacc agaatttaga gcaggacttg aacatatagg aagagcaaag tttaatttat
      601 ttggaaagaa tgtaaatact tctgaagaaa ctctcaatag aaatatagaa gaaattgtag
      661 aagctttata ttcactttct agacaaaaaa taggagctct tatcataatg gagagagaaa
      721 ctagaataag tgatataata aatacaggaa ctattattga tgctgaaatt tctaggcaac
      781 tattaataaa tatatttata ccaaatacac cattacacga tggagctgtt gtaattagag
      841 attctaaggt aaaagcagct gcatgttttt tgccattgac ggaaagtaag gatttaagta
      901 aagatttggg tactagacat agagcaggaa taggggtaag tgaggtgtct gactgtataa
      961 ctctgattgt atcagaggaa acaggtggcg tatcaattgc taaagcaggt aagttatata
     1021 gagatatttc tagagaaaga atgatgaata tattacgtag taatttaaaa acaaatacag
     1081 aaacaagaag ttttttcaaa ggtggtatat ttaaatgata aatagactaa aaaataatac
     1141 caaaataaaa ataatatcat ttttaagtgc aattgcatta tggatgtatg ttatggctgt
     1201 agttgaccct gaagaaacta aagggtttga agatatacct gtatctataa gtaatatgag
     1261 tgatttaaag gataaaaatc ttgttatata tccagacgaa aaattaacag cagatgtata
     1321 tataaaaggt agtttatcaa aggtacaaag tgttaagaaa gaagatatac atgtatatgg
     1381 aaccattgaa gacccaatac aaggacagaa ggaagtatat ttaaaagcaa atattcctca
     1441 aggtgttact ctagaattta agcatgatgt tttagtagta aatcttgaaa aaaacataaa
     1501 agaaaaaaga gatataaagg tagaagtaag tggaaattca aaaagaaata taaataaaat
     1561 tgaacttagt aaggaatttg tcgatgtatc aggtccaaga actctagtta aagaagtcaa
     1621 aagtgtaaga gctaatctag atgttggtaa tgagacagat gatttttcta aaaaattgca
     1681 attaatacca ttaaatagtg atggaactaa ggttgaagga gtaacattaa gttcatcctt
     1741 tgtgactgca aagatagaac tattgaagga aaaaattgtc ccaataagag taagtctaaa
     1801 tgataataaa gataatgatg tactgaaaaa ctataaatta agtaaggaac aagttacaat
     1861 aaaaggaaag aaagaagata tagacaaaat aagttatatt aagacaaaac ctattgactt
     1921 agctgatttg gctaatggtg aagaaaaaga agttagctta gaaattccag agggtataac
     1981 tcttgaggat agctctataa caattaaatt aaatgataca aaacaattga gtgcagaatt
     2041 tttatataat gcgggagata ttgaattaag aaatatccca acaaatatag ataatccaaa
     2101 tgtaatttct gatgtagata taaaagttgt tgtagagtca acggaggatt tgagtaagtt
     2161 aaataaaaaa gatatttcat tatatattga tttatcacaa ggccctgagg aagatagtaa
     2221 atataaaata aaatatgaaa caacatatca atttaaaaaa ataaatatag aacctaatat
     2281 tgttgaagtg gaatgaacaa atatttcgaa tatttagttg ttacaagatg cgacaaaaaa
     2341 acctataaaa ttaagtttgt taattttatg tttttgttgc atcttgttta ttttagttat
     2401 attataaaat aaggacaatg agatatttgt taaatctaaa aagcgctaga tatatgaaaa
     2461 aagactgata aatagattta aataaatatt ttgaattaaa tatttcaatt atctctagga
     2521 aaacgttgct gaacgagtta taatttaatt gagatataaa aagttctatt tcaaaaaatt
     2581 caagatgagg tggaaatgca tgagaagttt tgaagaagta attaagtttg caaaagaaag
     2641 aggacctaaa actatatcag tagcatgttg ccaagataaa gaagttttaa tggcagttga
     2701 aatggctaga aaagaaaaaa tagcaaatgc cattttagta ggagatatag aaaagactaa
     2761 agaaattgca aaaagcatag acatggatat cgaaaattat gaactgatag atataaaaga
     2821 tttagcagaa gcatctctaa aatctgttga attagtttca caaggaaaag ccgacatggt
     2881 aatgaaaggc ttagtagaca catcaataat actaaaagca gttttaaata aagaagtagg
     2941 tcttagaact ggaaatgtat taagtcacgt agcagtattt gatgtagagg gatatgatag
     3001 attatttttc gtaactgacg cagctatgaa cttagctcct gatacaaata ctaaaaagca
     3061 aatcatagaa aatgcttgca cagtagcaca ttcattagat ataagtgaac caaaagttgc
     3121 tgcaatatgc gcaaaagaaa aagtaaatcc aaaaatgaaa gatacagttg aagctaaaga
     3181 actagaagaa atgtatgaaa gaggagaaat caaaggttgt atggttggtg gaccttttgc
     3241 aattgataat gcagtatctt tagaagcagc taaacataaa ggtataaatc atcctgtagc
     3301 aggaagagct gatatattat tagccccaga tattgaaggt ggtaacatat tatataaagc
     3361 tttagtattc ttctcaaaat caaaaaatgc aggagttata gttggggcta aagcaccaat
     3421 aatattaact tctagagcag acagtgaaga aactaaacta aactcaatag ctttaggtgt
     3481 tttaatggca gcaaaggcat aattttaagg gggaaatagg caaatgagca aaatatttaa
     3541 aatcttaaca ataaatcctg gttcgacatc aactaaaata gctgtatttg ataatgagga
     3601 tttagtattt gaaaaaactt taagacattc ttcagaagaa ataggaaaat atgagaaggt
     3661 gtctgaccaa tttgaatttc gtaaacaagt aatagaagaa gctctaaaag aaggtggagt
     3721 aaaaacatct gaattagatg ctgtagtagg tagaggagga cttcttaaac ctataaaagg
     3781 tggtacttat tcagtaagtg ctgctatgat tgaagattta aaagtgggag ttttaggaga
     3841 acacgcttca aatctaggtg gaataatagc aaaacaaata ggtgaagaag taaatgttcc
     3901 ttcatacata gtagaccctg ttgttgtaga tgaattagaa gatgttgcta gaatttctgg
     3961 tatgcctgaa ataagtagag caagtgtagt acatgcttta aatcaaaagg caatagcaag
     4021 aagatatgct agagaaataa acaagaaata tgaagatata aatcttatag ttgcacacat
     4081 gggtggagga gtttctgttg gagctcataa aaatggtaaa atagtagatg ttgcaaacgc
     4141 attagatgga gaaggacctt tctctccaga aagaagtggt ggactaccag taggtgcatt
     4201 agtaaaaatg tgctttagtg gaaaatatac tcaagatgaa attaaaaaga aaataaaagg
     4261 taatggcgga ttagttgcat acttaaacac taatgatgct agagaagttg aagaaagaat
     4321 tgaagctggc gatgaaaaag ctaaattagt atatgaagct atggcatatc aaatctctaa
     4381 agaaatagga gctagtgctg cagttcttaa gggagatgta aaagcaatat tattaactgg
     4441 tggaatcgca tattcaaaaa tgtttacaga aatgattgca gatagagtta aatttatagc
     4501 agatgtaaaa gtttatccag gtgaagatga aatgattgca ttagctcaag gtggacttag
     4561 agttttaact ggtgaagaag aggctcaagt ttatgataac taatgataaa agaataagaa
     4621 tcataactgg tcactatgga agtggtaaaa gtgaatttgc gatgaactat gttgttaagt
     4681 taagagatat ggtttctggc aaagttgcta tagccgattt agatgtggta aatgtatact
     4741 ttagaactag agaaaagaaa gagttaatga aatccttagg tatacaacca atagatagct
     4801 caattaatgc tccaacatta gatttaccag cagtatcagc agaggtaatg tctcctatgg
     4861 tagaccattc atacaattct gttatagatt taggtggaga taatgtaggt gctagggtta
     4921 taggaagatt tagtcatttg ttaaaagaag gcgattatga tatgcttttt gtaataaatg
     4981 caaataggga gaaaactcaa acttctgaag aagttatcca atatataaaa gaaatagaaa
     5041 aatcttctaa attaaaggta acaggtctaa ttaataatac tcatcttatt aggtttacta
     5101 ctattgatga tgttttaaga ggtcagaaag ttgcaaaaga agtttctgaa aaatgtaata
     5161 taccgataag atatgttgca tgtttagaaa atcttgttga acagttacca aaagatttag
     5221 aaggagaaat ttttcctata aaattatata tgagagaaga ctggatgtag tttgagctca
     5281 aatatataga aagtttaagg aggcttaggt aaatggctaa aggaaaagta tcttttaatc
     5341 aagaacgttg taaaggttgt ggattgtgtg ttgaagcatg tccagtaaaa atcatacaat
     5401 tagactcaaa tgtaataaac aaaaaagggt ataacccagc aactgttttt gaaatggaaa
     5461 aatgcatagg atgtgcaagt tgtgcaacta tgtgtccaga tgtagttata acagtagaaa
     5521 gagattaatt taattaatat ttaaggggga attttaatgg ctaagatact tatgaaaggt
     5581 aacgaagcat ttggaaaagc agctatagaa gctggttgca aatatttctt cggttaccca
     5641 ataactccac aaagtgaatt accagaatat ttatcaagag agttacctaa aataggtgga
     5701 gcgttcgttc aagctgaatc agaagtttct gcgataaaca tggtttatgg tggagcaggt
     5761 gcaggagcaa gagttatgac ttcttcatct tcaccaggag ttgctttaaa gcaagaaggt
     5821 ataacatatg cagtaggagc agaagttcct tgtgttgtac ttaatgtaat gagaggtggt
     5881 ccaggacttg gaagtataca accttcacaa gctgactact ttatgtctac aagaggtgga
     5941 ggaaatggag actacagaac tccagtattc gcaccagcta cagttcaaga agctgttgat
     6001 atgataatgg aagcttttga cgttgcagat tattacagat caccagttat ggtagttgca
     6061 gatggtatga taggtcaaat gatggagcca gttgaattta gagctcctga gaagaaaaga
     6121 gaattgcctc ctaaagattg ggcaactgtt ggaactaaag gaaaaagaaa acctaatgta
     6181 ataaactctc tatacttaga accagaagta ctagaagacc attgttggca tttacaagaa
     6241 aaatttgatg caatggaaaa gaatgaagta caatacgaaa tgtacaaaac agaagatgct
     6301 gaatttgtat ttgcagctta tggaactact tcaagagtag taaaaagcgc aattgatata
     6361 ttaagagaag aaggaataaa agcaggtctt ataagaccta aagtattatg gccattccca
     6421 ttcgaagcat tcaaccaaat tcctaatgct agaaacatat tgactgttga aatgagtatg
     6481 ggacaaatgg tggaagatgt taaaatggca gtagaaggta aattaccagt ttacttccat
     6541 ggaagaccag gaggaatgac tccaacacca gcagaaatag ttgaaaaagc taaaaaaatt
     6601 attgccggag aattggttgc aggaggtgct agataatggc tgttgtattt aagaaaactg
     6661 aaggattaca agatactcaa actcactact gtccaggatg tacacatggt ataatccata
     6721 gattagttgg tgaagtatta gaagaactag gagtattagg tgatgctgta ggtgttgttc
     6781 cagttggatg ttcagtttta ggatataaat attttaactg tgacactcaa gaagctgctc
     6841 atggtagagc gccagctgct gctacaggaa taaaaagagt tcatcctgaa aatacagtat
     6901 tcacttatca aggtgatgga gacttagctt caattggtac tgctgaaata gttcatgcag
     6961 ctgctagagg agaaaaaata actactatat ttgtaaacaa tacaacttat ggtatgactg
     7021 gtggacaaat ggctccaact acattagttg gacaaagagc tactactgct caatcaggaa
     7081 gaaatgctga aactcaaggt tatccaataa gagtaagtga aatgttagct acattaacag
     7141 gagctgtatt tgtagaaaga gtagcagttg atactccagc tcatgttaga caagctaaaa
     7201 aagctataaa gaaagcattc caagttcaac aagcaggatt aggatttgga atagtagaag
     7261 tattatctac ttgtcctaca aactggggac tagctccaaa cgatgcttta caatggttaa
     7321 gagacaacat gataccatac tatccattag gaaatttcaa aaatgttgaa gttgaggagg
     7381 tgaagtaaat gtctacagct agagtaatat gtgcaggctt cggaggccaa ggtgttatgt
     7441 ctatgggaca attacttact tatgcaggaa tgctagaagg aaaagaagtt tcttggttac
     7501 catcttatgg accagaaatg cgtggaggta cagcaaactg tgcagtaact gtatctaatg
     7561 agccagttgg ttctccactt ataacagatg atgcaactgc agctatatta ttaaatatac
     7621 cagcatttga aaaattcaaa gatgatgtta ttcctggtgg aaaaataata gtaaatagtt
     7681 ctttaataaa agaaaaagtt gatagaacag atgtagatgt ttattatata ccagcaaatg
     7741 aattagcagc tgaattagga aatgataaag ttgctaatat gataatgtta ggcgcttact
     7801 taaaagtatc tgatactgta gatatagaat ctgtactaga agcattcaaa aaagttttcg
     7861 gacctagaaa agaaaaattc gttccattaa acagagaagc tttacaaaaa ggtatggatg
     7921 ctacatgttc taaagcaact aactaataaa gaaaaagatg tcaaaaaaaa gacaatagaa
     7981 ttatttctat tgtctttttt gctgataagt attaaaatgt ttttatgact gaaattaagc
     8041 ctataatgat attttatgaa ctataaaaat ataatacaaa ttacaagctt ttcaattatg
     8101 aaaggagaat ctttagtgag aaagtatttt ggaacagatg gagttagagg agtagcaaat
     8161 acagaactaa catgtgattt agcatataaa cttggtagag ctggtggatt tgtcttagct
     8221 caaggagacc acagagtaaa agtagtagta ggtaaggata ctagaatatc aggtgatatg
     8281 ttggaagcat ctttaatagc aggacttatg tctgttggat gtgatgttat tactgttgga
     8341 ataataccaa cacctgcagt ggcatattta actagaaaat atggtgctga ttgtggtgtg
     8401 gttatatctg catctcacaa tcctgttgaa tataatggaa tcaaattctt taataagaat
     8461 ggatataaat tagatgatga aattgaattg aaaatagaag aatatataga cgatatagac
     8521 aaaattgatt gtcttccaat tggtgaaaat gtaggaagaa aattacatga acattgtgca
     8581 caaagagact atgtagacta cctaaaatca ataataagca ctgactttaa gggattaaaa
     8641 gtagttttgg actgtgctaa tggtgcgtca tataaagtgg caccaatagt gtttgatgaa
     8701 ctaggagcaa gtgtaatttc aataaatagt tcaccagatg gaaataatat aaactataaa
     8761 tgtggctcta cacatccaga acaacttcaa agggctgttt tggaacataa tgctgactta
     8821 ggtcttgcat atgatggaga tgctgataga ttaattgcag taaatgaaaa gggtcaaatt
     8881 gttgatggag accatataat gatattgagt gccttaaatt taaagaaaaa taataaattg
     8941 gcgcaagata ctttggtagt tacagttatg agtaatatag ggttgacaat agctgctaag
     9001 gaaaatggaa taaatttatc tactacagca gttggagaca gatatgtttt agaagatatg
     9061 gttaaaaatg gatataatct tggtggagag caatctggac atatgatatt tttagattac
     9121 aacactactg gagatggagt gcttagttct ctaatacttg caaatataat cttgcaagaa
     9181 aagaaacctt tatcagaaat tgcatcaata atgagccaat acccacaagt attagtaaat
     9241 gcaacaataa aaaatgaaaa taagaacaaa tatatggaat atccagaaat aaaaactgag
     9301 atagaaagaa tagaatctat tttaaacgga aatggtagag ttttgataag accatcagga
     9361 actgagccat tagttagagt tatgttagag ggtaaagagg aaggtcaaat aaaagaatta
     9421 gcaacaaact tagctaatct tatacaagaa aaattatcgt aatttaaagg gtattctcag
     9481 atacaaatac tgaggacacc tttctaagcg ccagaactta gttatagaca taattattga
     9541 gtaatatagg aactaagttg acgaggtcag agttaatcga attgtcggcg gatgctctgc
     9601 ggtgtgttta ccatcgaaag acttctttaa ataataggag caatcctatg gacaaaggga
     9661 agccaaagta aacttatatg acctaactct taaaatatat aaataatatt tttaggagga
     9721 cacaaataat gtgtggaata gttggatatt taggaagtag aaaggcagca gaagttatag
     9781 tagaagggct ttctaagcta gagtacagag gttatgactc agcaggagta gcagttaata
     9841 gttctaatga agaagaactt aatattagaa agtttaaagg tagactttct gtacttgcag
     9901 aagacttaga aaagaatcct attgatggaa atttaggtat agggcatacg agatgggcaa
     9961 ctcatggaga accatctgat gtaaactcac atcctcattt taatcaagcc aaaacaatag
    10021 cagttgttca taatgggata atagaaaact atatggaaat aaaagaagaa ttaatttctg
    10081 aaggtgttaa gtttgaatct caaacagata cagaggtaat agcacattta gtagataaat
    10141 actatgaggg taatttatta gatgctgtat acaaaactat atcaaaatta agaggagctt
    10201 atgcattagg tgttatatgt aaagaacatg gaaatgaatt agttgcagta agaaaagata
    10261 gtcctcttgt agttggtgtt ggtgaaggtg aaaactttat agcatcagat atacctgcat
    10321 tattaaaata tactagagat gtttatttct tagaaaatgg agaagtagtt catctaaaag
    10381 acgaaaatgt aacagtttat gactctaata gaaatcttgt tgaaaaagaa gtatttcatg
    10441 taacttggga tgtagaagct gcgtctaaag gtggatatga ctactttatg tcaaaagaaa
    10501 ttcatgagca accaacagga gttagagaaa ctttagaaag aagacttgat gataatggaa
    10561 atataattct agacagtata aatatttcta aagaagactt agaaaaaata aataaagttt
    10621 atatagtggc ttgtggaaca gcatataatg ctggattact tggtaagtat gccatagaaa
    10681 aatttgtaaa tataccagta ataactgata tagcatcaga atttagatat agcgacccat
    10741 ttgtagatga aaattcatta gtaatattag ttagccaatc tggtgagaca gcagatactc
    10801 tagcagtatt aagagattca aaggctaaag gagcaagaat actttcaata actaacgttg
    10861 taggttcatc tatagcaaga gaatcagatg atgtattcta tacttgggca ggacctgaag
    10921 tagcagttgc atcaactaag gcatatacta ctcaaataac ttctttatac atgatagctt
    10981 tagactttgc tatcaaaaaa ggaacaataa ctagagaatt ttatgatagt atgatttcta
    11041 agatgaaaga aataccatca aaaatacaag aaatattaga caatgaagaa tatataaaag
    11101 aagttgctaa aacagttgtt agttctgaac atgcattcta tttaggaaga ggtatagact
    11161 atagcttagc aatggaaggt tcattgaaat taaaagagat ttcatatata catgcagaag
    11221 cttttgcagc aggagagcta aagcatggaa caatagcttt aatagaaaaa ggaactcctg
    11281 taattgccat agctactcaa gaaaagttat ttgaaaaaat ggtttctaat atggaagaag
    11341 taagagcaag aggagcttat gtagtagcaa tagctcaaag ccataataag gatgttgaga
    11401 aagctgctga taaaataatc tatataccaa attcagatga tattttatca ccaatattag
    11461 cagttgtacc aatgcaatta cttgcgtatc atgtctctgt actaagagga tgcgatgtag
    11521 ataagccaag aaatctagct aaatcagtaa cagttgaata aaaacagatt aaaaattaaa
    11581 tagataatat ttattgaaaa aaactcatcc tgttatttgt attttatagg atgagttttt
    11641 tagataaata ttaaatttgt tttgtttttg ctataataaa ttttataaaa agattgatat
    11701 taaatcatct gtaaaatgat aaatatatag accataccag gtatagggaa cgataaatat
    11761 agttcatata aaatatagtg agtacttaat aaacatatag tcaatatcag acataaaaaa
    11821 ataatagctt atgagaaaaa ttaaaataca aggtattagt aaccttgtga taagataaag
    11881 tgagtagttt aaaataagaa tataaggaac tatttataaa agaatataat gaagctaata
    11941 gagtataaga aaattacttt attttagtaa aataatttca aataaaaatt tttttaaatt
    12001 gggagtataa attatgacaa atgattttaa aaaagaaaag aaagaagatt attttgttaa
    12061 tcttaaggca atatctactg aagttttaca agaaaaaatg caagataacg cttgggttat
    12121 agttgatact cgcttaaatg atgcttataa tggctggaag ttagatggag taaaaagagg
    12181 tgggcatata aaaagagcag tagatttttc tgctaattgg ttaaatgtat actcagatag
    12241 aaaagatgaa gtgctagaac aagcattgaa aactaaaaga attgatttag ataaaaatat
    12301 agtattgtat gatgcaaatg gaaaagatgc tttggttgta gcagattatt tatctaaaaa
    12361 aggatataaa tatctatata aatatgatat taaacaatgg gcagacgatg aaaatcttcc
    12421 aatggaaaga tataaaaatt atcaaatgat agttcctgct tttataataa aagatatatt
    12481 agatggaaaa attcctgaga catttgaaga ttcaaagaat ataaaaatga ttgaagcgag
    12541 ttggggagaa gaatcgtata caaaagggca tatacctaca agtgttcatg taaatactga
    12601 tattatagaa cctcctccaa catggatgct tgataatgat gataacttaa ctaagtttgc
    12661 tttagactat ggactaacaa aggatgatac tgtaatagtt tcaagtatta cacctatggc
    12721 atcttataga ctagctgtta tattgagata tataggagta aaagatgtaa gagttttgaa
    12781 tggtggaaca aactcatggt tatcagcagg atatgaatta gaatttatta gtaatccaaa
    12841 acattcatgt actaattttg gagcagatat accagttaac tcacaattag ttgttactac
    12901 atctgaatta agacaaaaac taaaagaaaa aaataagttt atactagtag ataatcgtac
    12961 ttgggatgaa catataggaa aagtatctgg atatacatat tatgataaaa aagggcgtat
    13021 accaggagct ttatatggac attcaggtag taatagtgtt tcactagaag aatatcgtaa
    13081 tattgataat acaatgagaa ataaatctga gattttagaa atgtgggaca aggaaaatat
    13141 agatgtgaat aaacagctta tatttatgtg tggaagtgga tggcgtgctg ctgaagtatt
    13201 aacatatgca aatgtcatag gagtggaaaa tacctccctt tatagtgatg gatggatggg
    13261 ctggagtctt gataattcaa atttaattga agtgggagaa cacaaataaa gtttttgaaa
    13321 gtacaataga gttgtttaaa acaaaggtgt ttttgattta ttaggaagag tgtgtaaatt
    13381 gtgtattggg aagtgtactt aggtatgttc atttggagct atggaaattg ataaaaaagt
    13441 attatctgtt tattttgctg gtaaaatatg aatagattat agattataga ttatagatta
    13501 tagattatag attatagatt atagattata gatttaagta tagactaagt aatttatatt
    13561 acagaagata atatataaga attaatgcaa gatgtatttg atacatataa ggaattggca
    13621 agtccaggag aaggatagag aaaattgaga ttgaagagtt tgaaagagat tattagatga
    13681 aaaatacaaa aatgtataaa aatacatatt gagaaagtat atctctctta aatttgttaa
    13741 taattctatt atacagaaaa acatatttaa gggggatttt ttactatgaa aaaagtaaaa
    13801 ttagctataa gcttttttat attaatgatt ataggaatga caatatctta tgcagataca
    13861 agtataaata ctagatacaa tagattaagt aaagctcttg aaatatcaga agcaaatttt
    13921 aaattttata atgttaaaat taattctgtt ataaatcata atttatcaaa taaagaattt
    13981 cataatattt ttatagaaat aattaacaat ctaaagttgg ataaaaaagg aataaattgg
    14041 aatcaaaaag ggtctggcga taaaatacaa ttatgtgcta ctataaaagg tagcgataaa
    14101 gaaatttcta taaaaggaat aaataaaaat aacaaagaaa gttacatagt agttgacata
    14161 ttggataaca aagtatataa acataaagag gttatttata gtaacgtaga aaatgtgtta
    14221 aataaatatt catctcaagt agatgtgtct gcttgtattg ttggagaata cacaaaaagg
    14281 ttacaaaaca acaaatatga tgatatttta caaaaaatat tatataatat gagtgctaag
    14341 caaatagatg aagttaaaga cgtaaacttt ttatctataa cagcatatag taaagtatta
    14401 aatgattatg aattagaata tttaggggaa aaaattaatt taaatatagg aattaaatat
    14461 aatgaggatg acggaaaaac actcatatat attgccacac caattataaa attagattat
    14521 taattatgag gagatcaaaa aatatggcta agataatagt aaaaaagagt aatccactta
    14581 aaggtagtgt taaaatagat ggtgcaaaaa atgcagtttt accaataata gcagcgactt
    14641 tattagcaaa cggaaaatca acattaaatg gagtaccaaa tttaagagat gttcatgtta
    14701 tatcagattt attaagacat gtaggagcag aagttgaata taaagaaaat acacttactg
    14761 ttgatgcaag caatataaaa acttgtgaag ctccatatga gcttgtaaga aagatgagag
    14821 cttctttttt ggttatggga ccattgcttg ctagatttaa tagtacgaaa atttctatgc
    14881 caggaggatg tgctatagga acaagaccta tagatttaca tttaaaagga tttaaagcct
    14941 taggtgcaaa gatagaaatg gaccatggtt ttgttgaagc agcaacagaa aagttagtag
    15001 gaaataaatt atatttagat ttcccatctg ttggagcaac tgaaaacata atgatggcag
    15061 cttctttagc agaagggact acaataatag agaatgcagc agaagagcca gaaatagtag
    15121 atttagcaaa cttcttgaac gaaatgggag cagatgttaa aggtgcaggt actaatacta
    15181 taaaaataaa aggtgttaaa gagttaaaag gagcagaaca taatgtaatt ccagacagaa
    15241 tagaagcagc aacttatatg gttgcagcag ctatgacaaa aggtgatata actgttgaaa
    15301 atgttttaat ggagcattta aaaccagttg tagctaaatt aagagaagct ggttgcgaaa
    15361 taacagaaat ggacaattct gtaagagttg taggtccaaa agtattaaaa ccaatagata
    15421 taaaaacttt accacatcca ggatttccta cagatgttca agcacaattt atggctatgc
    15481 ttactgtagc caatggaact ggtgtagtta tagaaactgt ttttgaaaat agatttatgc
    15541 atgttgctga atttaataga atgggtgcta atataaaaat agatggaaga agtgcggttg
    15601 ttaatggtgt agatgagcta cacggtgcag cagtaaacgc tactgattta agagcggggg
    15661 cagcccttat tttatgcggt cttatagcag aaggagaaac tcaaatagga gaaatatacc
    15721 atatccaaag aggttatgtt gatatagata agaagataac agcactaggt ggtcaaatcg
    15781 aaatagtaga agattaataa ttggtttata gaagaaagtt ataaattaat agggttgttc
    15841 atatacttaa atacaaggtt ttaaaagcta aggaggagag tatatgaaga acccattggt
    15901 tgttttgtta ggatttgtga cttgctctgt gttagttcca tcacttataa ctttagtttc
    15961 ttataaaaat gtagaattaa ctgaaaaacc agagagccca gtatctataa acaaaactat
    16021 taaaaaaagt gatattgaag acaaaggtaa taaagaagaa aaaagtacag tgagctatga
    16081 aactgtaaat aaaaaagcac caattatcaa tgtatataat catataacag ggaaaaccga
    16141 aaagatggac atggagaact atttatgtgg agtactagct ggtgagatgt cttcagagtt
    16201 tgatatagaa gctttaaaag ctcaatctgt agcagctaga acatatgtag tatataaaca
    16261 ggaacatggt aaatctagta aacataaaaa tgcagtagta tgtactgatt ataaacattg
    16321 tcaagaatat aagagttatg atactcttaa aaaattaaat ggtgaagaat ggattaagaa
    16381 taaatattcc aaaatccaag aagcagttag aggaactaaa ggtcaaataa taacttataa
    16441 tgataaggcg atacttcctc tttatttttc tacatcttca ggaaaaacag aaaatagtga
    16501 agaagtattt tctgcaaaat atccttattt aaagtctgtg gaaagtcctt atgataagta
    16561 ttcccctaaa tttgcgtcaa cacttaaaat aagtaacaca gattttgtta aaagtcttag
    16621 aagggcatat agcactattg tgatagatgt gaataattta agtaaacaag tttcaataac
    16681 taaaagaagt gatgccggaa ctgtagaaaa aataaaatta ggaaataaag aattgactgg
    16741 aaaagacatt agaactgttt ttaaattgaa ctctgcaaac tttgacataa aatttggaga
    16801 aggatacata gatttcgtgg taaaaggata tggtcatggt gttggtatga gccagtgggg
    16861 agcagaagga atggcagaag agggctataa atattatgat atattatcac attactacac
    16921 agatacaaaa ataaaagata tatactaata attgtataaa atgccaaaaa cacgtcaata
    16981 atccaaatgt agaaatatat ttggaggtgc aaaatgaaga aaaagctgtt agaaaaagat
    17041 ggtttttatt tatctttgtt tgtatgtgtt tgtttattag cagtaggtgg agtttggttt
    17101 acaaataata atgtagataa attggcttct aataaaggaa ttatggaaaa tgctaataaa
    17161 gatagtgaag aggaaataca tttaattgaa aaagacaaga aagatgctat ccctactgca
    17221 acggattcaa aacaaaactt agaaaaagct aagtcaaaag aggaaaataa atcgagtaca
    17281 acaaaattaa attacattgg agataaagta ataagaggat attcagaaaa agaacctagt
    17341 tattctaaaa ctcttgatgt ttgggaaact cataaaggtg tagatattag ttgtactaaa
    17401 ggcaaagaag taaaatctct attaaacgga atagtagtag atgtatttaa tgatgaagaa
    17461 tatgggcaat cagtcaaaat aaaaagtgac aataacattg tagtagtata ttcaaatcta
    17521 gataaaaatg ttagtgttaa gaaagaacag aaggtaacag aaggacaatc tctaggtact
    17581 gtaggtagta cttctcaaat agaaagtgaa gaaggtatac atgttcattt agaggcgtat
    17641 agtggagaaa aatctataga cccaatgagt ctaattaagt aaaagtataa caagtttaat
    17701 catatttggt taagcttgtt atattttttg gtgtttttac atatatatca ttaaagctat
    17761 tatgtgaggg gggtaatctt tgagatctca tatagaggaa agggccatag tggtagcaaa
    17821 atatatactt gaaaaaaaca ctacagtaag acaaaccgcc aagacatttg gagtaagtaa
    17881 aagtacaatt cataaggatg ttacagaaag attgaaggaa ataaatccat cccttgccaa
    17941 agaagttaaa aatgtgctgg acaaaaataa atcagagaga catattagag ggggattagc
    18001 aacgaaatta aaatatgaga aggaacataa aaaaatgtag gttaaaaacc tacttttttt
    18061 attgaattaa attataaaaa gaaatatatt aagtaattat aaatagcaaa ataataatac
    18121 tctcaaaaga tttattttct cttgattaaa gcgagatttt tgtatatgct ttattatagg
    18181 agtattaaaa taggcttata atataatcta atattataaa taaaggagtt gaaaaaatgg
    18241 ctggagctga tataggcata gatttaggta ctgcaaatgt gcttgtttat gttagtggta
    18301 agggaatcgt tttagaagaa ccatctgttg tggcgataga caaaagaaca gattctgttt
    18361 tagctgtggg agaagaagct aaaaagatga tagggagaac accaggtaat atagtcgcta
    18421 taagaccttt aagagatggt gtaatttcag attatgatgt aactgagaag atgttaaaat
    18481 cttttataga taagatagtt gataaaaagg gatttggaag attttttatg ccaagaatta
    18541 tggtatgtgt accaacaggt gtcacagaag tggaaaaaag agcagtagaa gatgctacga
    18601 gacaagctgg agctagagaa gtatatatca tagaagaacc aatagcagct gccataggag
    18661 ctggtgtaga catatcacag cctaatggta atatgattat agatattggt ggaggtactg
    18721 tagatatagc tgtaatatct ctaggaggag ctgttgtaag tgaatctata aaggttggtg
    18781 gagatagatt tgatgatgct atagttaaat atatgaaaaa acaacacaat cttcttatag
    18841 gagaaagaac agcagagaaa atcaagtttg aaataggttc tgcatttaag agagaagaag
    18901 aaaaatatat gaagataaca ggaagaaatt taattacagg acttcctagt tctataacta
    18961 taaattcaac agaaatgttg gaagcactta gagaatgtgt tgagcaaata gtagttgcta
    19021 ctcatgctgt tttggaaaaa acgcctcctg agttagcagc agatatagga gatgtcggta
    19081 taataatgac tggaggaggt tcattattat atggattaga taaaataata gaacagagga
    19141 caggaataac tgttacaata gcagatgaac cattatcttg tgttgcaaaa ggtacaggaa
    19201 tagctttagg ttcaatagac ttacttgaaa ctggtgggtc tttcaaaaga aaataattag
    19261 gagagtttag aatgttaaat atagatgaaa taaaaaaatt aataccacat agatatccat
    19321 ttttattagt agacaagatt acagagctag aagttgggaa aagagctgtt ggtataaaga
    19381 atgtaacagt taatgaacca tttttccaag gtcactttcc agaatatcct ttgatgccag
    19441 gagtactaat agtagaagct ctagcacaag tatgtggagt ggcaatgatg tctgtagagg
    19501 aaaacaaagg aaaactaggt gtatttgcag gtatagataa agttagaatc aagagagaag
    19561 taagaccagg agatacatta actatggaaa ttgaaatgac aactcttaga aaaaatatag
    19621 ctaaggcaga tgctaaggca tatgtaggtg aagaacttgt ctgtaaaggt gaattaatgt
    19681 ttgctttagt agaaaaataa aaacttgaaa tataaaagta aaaaggtaga taatttggag
    19741 tatttaatca gatactctaa aatatctacc tttttatata aagaatttta tcatttagtt
    19801 tatatttaga ttaggtggat actaataaag ccaaggctat tgtttcagct aaatctaata
    19861 taaagctaag gcgtatatta ttaaatgaaa gtttattatt ttcatcaatt ttatctacaa
    19921 ttcctactat agaataattt cctatttgag gtagtttttt accaacacct tttccgggca
    19981 ggataggtcc ttctctaatc tgtatattac ctatgttact ctgcgacccc aagcaagcat
    20041 ctatagccaa gaagttacct tgtatatgtg tattttttat agtatctaaa gactcatata
    20101 tatttaaggc gtgaataggg ttatctagtg taccgtaaac ggggtattta aagttactgt
    20161 tcttaagtat tgttccaact aaagggccta aagtatcacc tatagccctg tctgttccta
    20221 tgcaaacaac aatcgtattt tcattaatta tagtttttag aactgaactt agcatctgta
    20281 taacatcatc atcgctgtag tttactttag ctaaatacaa taggaacacc cccttcaaaa
    20341 aaatatatga ataaaaagca ttaaaaagac ctaactttaa atttcagata gatagaaaat
    20401 tttatttgtc ttacaaaaaa aggaatagaa aaaatatgac taaaaatatt gacaaaattt
    20461 taaaaaaata gtaagctagt aaagtaaaac aaaacttaaa tcttgattac cttatcaaga
    20521 gaggctgagg gataggccct gtgaagccca gcaaccaccc aaatgggaaa aggtgctaat
    20581 tcctacaaga caaaagtctt gaaagatgag gtcagttaat tagtatgtaa ttacgattat
    20641 taacctcttc ttataaagga gaggtttttt tattttgagt aaatttctaa aattaaaaat
    20701 aaggtaaaac ctataataaa aattaagaat aaataagggg gaataaaaat ggcaagacat
    20761 ttatttacgt cagaatcagt tacagaagga catcctgata agatatgtga ccaaatatca
    20821 gattcaatat tagatgcatt attagaaaaa gacccacaat ctagagtagc ttgtgaaact
    20881 acagttacta ctggtttagt tttagtagca ggagaaataa gtacatctgc ttatgtagac
    20941 attcctaaat tagtaagaga aacagtaaga gaaattggat atacaagagc aaaatatgga
    21001 tttgattgtg atacttgcgc agttataact tctattgatg aacaatctgg agatatagct
    21061 atgggagttg atgaaggtct agaaagtaag actggagaag agatagaaga agaaatagaa
    21121 aaagttggag ctggagacca aggtataatg tttggatttg catgtaatga gactccagaa
    21181 ttaatgccac ttccgatatc tttggcacat aaattatcaa gaaggcttac agaagttaga
    21241 aaaacaggtt tagttgatta tttaagacca gatggaaaaa ctcaagttac tgttgaatat
    21301 gaaggaagta aagctgtaag agtacataca gttcttatat cagctcaaca ttgtgaaaca
    21361 gtatcaaatg ataaaataag agaagattta attaatcatg ttattaagga agttatacca
    21421 gcagaattac ttgatgaaga gactaaaatt tatataaatc caacaggaag attcgttata
    21481 ggaggaccac aaggagatac aggtcttaca ggaagaaaaa taataataga tacttatggt
    21541 ggatattcta gacatggtgg aggagctttc tcaggaaaag accctacaaa agttgataga
    21601 tctgctgctt atgcagctag atatgttgca aaaaatattg ttgcggctgg tcttgcagac
    21661 aaatgtgaga tagaacttgc atatgctata ggtatagcta gaccattatc tatatttata
    21721 gatacttttg ggacaggcaa ggtttcagaa gaaaaacttg ttgaattagt aaataaacac
    21781 tttgatttaa gaccgggagc gataataaga gacttagatt taagaaagcc tctttataaa
    21841 aaggttgctg cttatggtca ctttggtaga actgatatag atttaccttg ggaaagaact
    21901 gacaaggttg agcagttaag aaaagatgct ttaggtgaat aatttttaac taaaaagctg
    21961 ttagaatgta attttacgat tactttctaa cagttttttt attatgagtt aaatattttt
    22021 aataaatgtt aatttatcat catataatga ataaacagta tttaatctgg tcaatacatt
    22081 ttatataata ctctactaaa tttatacata ttatgggata tatgttaaag gatggttaag
    22141 aatgtgttaa ctaatgttaa ataattaaaa atatattgaa atatcacaat aatgagtcta
    22201 atataatctt aataaactta cactttaaaa ataattttaa ttggggggga gtagtgtatg
    22261 tcaggcaaag taaatattaa aacggttata agttttgctg gagcttatgt tgctactgta
    22321 ataggttctg gatttgcaac tggtcaagaa atactgcaat tttttacgtt ttatggatat
    22381 gcgggtatta taggtggaat aatctctatg gtactatttt catggtttgg agcagaagtt
    22441 atagataagg gaagagaatt gaaacttaag gagccaataa aaatttatca agtatattgt
    22501 ggaaaatatc ttggaacatt ttttgaatgg tttggtccat tgtttttatt tggaacatta
    22561 gtaataatga ttgcaggagc aggagctaca ctatcagagt actatggatt aaatccatat
    22621 gtaggtagga ttggtatggc aatcgtttcc ctaataactg tttcattagg tttgacaagg
    22681 ctttctaaaa tactaggtaa tataggacct ataataataa tatttacact tttagtagga
    22741 gcaattagtt tatttagcaa tattgatgct ttatcaaatg ctggaaatat ggttgatagt
    22801 ttaaatatta aaactgctac atcaaatgga tatttctcgg gagtacttta tacttgttac
    22861 aatgtaataa tagtaataac attcttgaca ggaatgggag cttctgcagt aaataagaaa
    22921 gatgctgtat ggggtggtat agtaggtgga gtagcactta tggcagcagc tataatgatg
    22981 aatttggcat tactttcaaa cataggaaat atatacacaa aagagatacc agcactttac
    23041 ttagctgata agatatctcc aataataggt attttattct cagtagtact tttactagga
    23101 atatacacta cagctgtacc attgttatgg tcagtaacta accgatttgt agaagatgac
    23161 caccctaaat ttaaaattat aacaatagtg gtaagtatat tagcttgtat aggaggttta
    23221 ctgccatttg ataagttagt aggtacacta tatccatata caggttacat gggaatttta
    23281 attttactat gtatgcttta tagaagaatt actaaaactg aggggtacaa ggagaataaa
    23341 tctgaaataa gttagtactg taatatactt atggaattta atttatactc taaatataga
    23401 gtaattaaaa taaaatagct atttaaatat ggtacctgta tttcatggta ccatatttta
    23461 tggatgcaga ttttttttgt ttctgataaa atgtataata gttggaaatt acgttaagta
    23521 taaaaatata aaacttttct acgattatca tagttttaat atataaaatt atactaacat
    23581 ttaaatggag cgattattag tatttttagg agataaaaat ggaaaaatta gaaggaatga
    23641 taagtgaaat agtctttaaa aacgaagata atggttatac aatagctcac ctggtaaatg
    23701 aaaatgatga aattgtggtt gtaggatgta tgcctacttt ggcaattgga gaaagcatag
    23761 aagttgaagg aaagtgggta aatcataaga tttatgggac tcaatttgaa gtgaatagtt
    23821 ttatgccagt aacgccatca tccttagaag gaatttatgt ttacttatct tctggaatga
    23881 tacatggtat tggtgaaaaa atggcaaaaa gaataattga taagtttggt gtagatacgt
    23941 tagaggtgat acaaaattca ccagaaaaat tacaagaagt agaaggtata gggagtaaaa
    24001 aagtaaaaca aatagtaaaa agttatgaag aagacagaga attgagaaat ataataatac
    24061 agctatctcc ttttggaata acaccaaatt actgcctaaa aatatataaa aaatataaaa
    24121 gcagtgcaat agaagttata aataaaaacc cataccaact tgctgaagat ataagaggaa
    24181 ttggatttaa agtagctgat agtatagcaa gtaaaattgg tatagataaa aactctaaag
    24241 atagaatttg tcagggaatc ttatacactt taaataaatc tttaagtaat gggcatacat
    24301 atttaccaga gcatgttcta atacaagatt cggaaaaatt attggagcta aatggagaaa
    24361 taataaaaga gtgtgtaatg atgctagttt ataatcaaaa aatacatata gagaaagtaa
    24421 acaatgaaaa tcttatatac cttatgccat actatttagc tgaaaatgga gtatgtagtc
    24481 aaatagtaaa attatcacag tatgaatttg aagacttaaa aatagatata gataacgaga
    24541 ttaatgtttt agaagaagat aagaaaatta agttagcaga aaaacaaatt ttagcagtca
    24601 aagaatctgt taatagtgga gtattaatta taactggtgg accaggaact ggtaaaacaa
    24661 ctaccattaa tgctatcata gatatatttg aaaataatgg taaaagtgtg actttggcag
    24721 ctccaacggg aagagcagca aagagaatga gtgaaacttc taataaagaa gcaaaaacta
    24781 tacataggtt gctagaaatg ggattttcaa cagatgatga tttaactttt tttaaggatg
    24841 aagaagaccc aataaattca gatgtaataa ttgttgatga ggtatcaatg gtagacatta
    24901 tcttaatgta caatttactt agagctataa agttaggaac aagagtaatt ttagttggag
    24961 atagtgacca attaccttct gtaggtgctg gaaatgtact taaggatatg attaattcta
    25021 atattataaa tgtagttaaa ttaaatgaaa tatttagaca agcgcaagaa agtatgataa
    25081 ttgtaaatgc ccataagata aacaatggag aaccacttta tctaaataca aaaggtaaag
    25141 atttcttttt tataagaaaa tcaactaatg aagaaatttt aaatgaaata ataggattgg
    25201 ttaatgaaag attacctaaa ttttataaag ttgataaatt aaaagatata caagttttat
    25261 cttcaatgag aaaaggtgaa ttgggagtta ctaatctcaa tatagagtta cagaagtatc
    25321 ttaacaaaaa agaaaaattt aaagttgaag aaagtttttc taaaagatta tttagagttg
    25381 gcgataaggt aatgcaagta aaaaataatt atacaaaaaa atgggaaaca gaagaccaaa
    25441 aagaaagtgg agaaggtatc tataatggag atataggata tgtttatcac atagataaag
    25501 ataagaaaac tatttatata ttatttgacc agactaaaat tgtatcatac ctatatgatg
    25561 aattagatga aattgaccat agtttttgta caacaataca taaaagccag ggaagtgaat
    25621 ttccagttgt agtattgcca atagcgtggg ctcctcctat gttacttagc cgtaatttat
    25681 tatatacagc agtaacaaga gcaaaaaaat tagttgtatt agttggagat gtgaaatatc
    25741 ttgaatatat gataaaaaat aatagagtta atcaaaggta ttcaaattta ggatataagc
    25801 taaataaatt taaacaagaa gggttgttga tagaataaat atgaaaatat gcaatgataa
    25861 ttttattaag aattttaaaa atatgattaa taaatgtttg gattttatat atcctgagaa
    25921 tataagttgc ataatttgtg ataaatccat aaagaaaaca aatacttatt caatatgtaa
    25981 aagttgtttt aaagaaatga attttataca agatggttgc atgaagtgtg gaaaaccaat
    26041 aatacgtcat tcaattgaaa aagaatttat tgaagagtgt agttattgtt ttaataaaga
    26101 cttttatttt gataaatcaa tatcttgtat tgaatataat gatgtgacaa aaaagatggt
    26161 gttagggctg aagtataacc aaaaaacctt tatggctaag tatatagctc aaataatgaa
    26221 agaaaaatta tatttagaaa acataaaatt tgattatata ttatttgttc cacttcataa
    26281 aaaaaggcta aataaaagag gttttaatca ggcacaaaaa atagcattta atttaagtaa
    26341 aatagtaaat attccattat tagattgcat aagtagaaaa aaatatacaa gaatgctata
    26401 taaattgaat aagaaagaac gaaaagaaga attaaaaaat gtatttgttg ttaaagaaaa
    26461 tgttaaatta ataaataata aaaatatact tttaatagat gacatattta ctactggtct
    26521 aacaacaaac gaaatctcta aacttttaaa gttatcaggt gctaataagg tttttgtatt
    26581 gactctactt acaaaagcaa atgataatta tattatggaa taatcttata tattgacaat
    26641 tttagtttaa aatattaaaa tgaataagtg atatttaggt ctgtggttga aagtccaagc
    26701 cagtcgcagg caaagggatc cacgtaagtt aacactaaaa attgggttaa tgatcatggt
    26761 gcggcttaga agtaagacct accgataata aaatcgagag agagtagtag tgcggcgaac
    26821 ccaagcgaac cctccagtag gcgagtgtgg ggtcaaaaac caggtcagct aaatatcata
    26881 ttaaaagaga gctagcatac ctttatgtat agctcttttt tatatataat aaatttaggt
    26941 tgagtcataa agaggtgata tgtttgaata aaagacaaga aaatttatta acaacattgc
    27001 tattaaaaga agagtttata aatgttaata agattgccaa tgattttgag tgttcagaaa
    27061 ggacaataag gaatgattgc aaatatattg atgaatggtt atctactttt tgtaatgctt
    27121 atatcaaaag aaaacctaat attggaatta aatttagtgg agatggaaca gacaaaatac
    27181 tggtaaatca gaagttgcga ggtgaagtta aaagagtaaa tagtgagctt cataagcaag
    27241 ttgagatatt aaatttacta ctttgtaata ataaaaaagt tactttaaat gatttatcta
    27301 ataagttgta cataaataaa aatattgtca gagaagaaat aagtaaatta tcagtagcat
    27361 taaataatta taatttaaat gtctcgacta aaaaaggcag tggtatatat attgaagggg
    27421 atgagaaaga cataagaata atgttagtat cattcttatt taaatacata gataaataca
    27481 gattaaacat agatgaaata gagtacttcc atatagtaga tataataatt gtaaaaaaca
    27541 taatgcttac tatagaggag gatatggata taagacttac agatatatca ttcaagcaat
    27601 tgatgctatt tttgcttata aatataatac gaataaggct gggaaattct ttaaaagtat
    27661 caaatactaa gaaagagctt aagctaaaaa tgcttatatt ggatatagaa gatgaattga
    27721 aatctaattt atccatagta ttaaattatg aagaaaaatt gtttatagaa tctttaatat
    27781 tgggtatgaa caaacaaata aatagtaata atattaagaa atctttatat ctaaatgaag
    27841 aattagcaag ttatacaaaa aaaattataa atttagtatc agaagaatca ggaataaatt
    27901 ttaataatga tatattactt tatgaacaat taatatgtca tttgaatgtt acaatgcatc
    27961 aaatgaaaag caatgtttac ttagaaaatc cacttttaga taacataaaa actaaatttt
    28021 gttttttatt tacagtaata tcaagttcaa tagaaattga attaaaagga aaacaaataa
    28081 cggaagatga aattggttat cttacacttc actttcaaac ctctctagaa agaaaataca
    28141 gcaaaaggga atcaaataaa aaggcaagta ttgtatgtcc atttagtttt ggagtaagta
    28201 tgctattaaa agtaaagata gaaaagagat ttgataacat aaaaattatt gaaacattaa
    28261 gagaggaaga tttaaaaaga gataacttta ataagactat agattttata attgcatttc
    28321 aagagtatga aaatatccaa aaaccaattt ttataacaac accattattt actgatgagg
    28381 atgagaataa acttaaagag tttacaaaca aaataaaaga aaaagacacc tcatataaac
    28441 ttatgaacag attaatgatg tctgattatt taataagaga gttagaacat gatgatattt
    28501 atgaagctat aacatattta actaatttat taattgaaaa acattatgca gaaaaagagt
    28561 atttacaatc tattataaca agagaaaagt catatccaac caatgttgga aaaggaatat
    28621 tattccctca tggagacatg aaatatataa aacaatctgt tttatgtttt gcaaggttaa
    28681 aaacaccaat aaaaattaga aatggaaagg atataaagat tatactttta ttagcatata
    28741 aaaaagatga tgatagaaaa gtatttagag aattatttaa agaaatatct aatttaacag
    28801 aggatgaaaa tatgctagat attctaagta aatgtgacat tgaaaaggta aaagatattc
    28861 ttatataaaa ttcataatga actatggata agcacaaaat tttccatgtg atggaaaaag
    28921 ttgtgcatgt ttttttgttt ttataagata ttatatgttt gtaaggtata aataatctaa
    28981 aagtagagaa gcaatttagg aggaaaaaat ggaagaaata gttttaaaga ttataattca
    29041 tgctggaaat gcaaaatcta tgctttatga agcacttgac tatgcaaagg aaaacgattt
    29101 taaaaaagca gatgaattaa ttgaaaatgc aaatgaagaa attttaaaag cacataaagt
    29161 acaaactgaa cttatacaaa aggaagctgg gggagataaa tcagatatat ctattttatt
    29221 gattcactct caagaccatt taatgacttg tatgagtgag agaaacctta taaaagaaat
    29281 gattatgtta agaaaagaaa ttcaaaaatt aaaataaaaa aattaagggg gatttaaaag
    29341 aatgaaaaga aaagtatatt tattttgtag ctttggtatg tcaacaagtt tacttgcaga
    29401 taaaatgcag aaggtagcag atgaacacaa tttaccaata gaagtagagg catttccgat
    29461 agcagaaatt gacaaaatag ttgaagaaaa aaatccagat tgtatattat taggaccaca
    29521 agttaaatac atgctaaagg agttaaaacc aaagtttgaa gctcaaggaa aacttataga
    29581 tataatcaat gaagttgatt atggaactat gaatggggaa aaagtattaa aacttgctat
    29641 aaagctaata aaatctaaac aagcgtagaa ttatagtaaa aaataatagg aaatgagggt
    29701 ggatagatga atagattgga aagaatactt atgccattgg ctgaaaaaat aggtaaaaat
    29761 aaattattaa ttgcaattag agatggtttt ttaatatcat caccattatt aataataggt
    29821 tcattatttt tattagtagc aaattttcct ataaaaggat ggaatgaatt tgtaggtcaa
    29881 atttttggtc cagattgggc tgtaaaattg caacaaccaa ctgtagcaac atttgaagta
    29941 atgactttac ttgcagtttt aggaattgga tattcttatg caaagcaaat agatgtagat
    30001 ccaatagcat cagctgcagt tgcaatagtt gcctttttta tagtaacacc gtttgtaata
    30061 ccatatacac cagaaggaac agaatcagtc tacatggtaa caggtatacc actaggttgg
    30121 atgggctcta agggaatgtt tgtaggtatg ataacagcaa taacctctgt aaaattattt
    30181 gctgcagttg taaaaaaagg atggacattg aaaatgccag atggagttcc tccaaccgta
    30241 tcaaaatcat ttgcagcatt aataccaagt gcaatagtta tggtagtttt ctttttagtt
    30301 aagatagctt ttgaagctac accatatgga agtatacatg aatttatatt taagttttta
    30361 caaatgccat tattaaaatt aggaaatagt ttaggtgcta tggcaatcgc ttatatattc
    30421 ttacatttat tctggttctt tggaataaat ggaagtagtg ttgttggggc tgtgtataat
    30481 ccaatattaa aaattctatc agctgaaaac ttagctgctt ttcaggctgg ttcaaaaata
    30541 ccaaacataa taacaggtca attccaagat atgtttgcga catttggtgg agcaggtagt
    30601 acactatcat tagtcatagc tatgttatta atatgtaaaa gtaaacgtat aaagtcaatg
    30661 ggaaaactat caattttacc aggaatattt ggtataaatg agccattgat atttggtcta
    30721 ccaataatgc ttaacccaat actattgata ccatttgcaa tagttccaac aataaatata
    30781 ataatagctt acttctgtat gtcagcagga ttagtgcctc ttacaaatgg agttcagtta
    30841 ccgtggacaa ctccaattat attctctggt tttttaacaa caggatggca aggtgcagtc
    30901 ttacaattag tgcttcttat actaggaata tttatgtata taccatttat aaaaatgcta
    30961 gataagcaat acttgagaga agaaaatgaa actgttgaag aggaagatga tgatatatct
    31021 tttgatgatt tagtattata ggaggaagat tatgaagaaa atagttatga tattggatca
    31081 aatacaagct ggagctggag gaaaagagaa aagtaatata cctcctgctg gaaaatcaag
    31141 cccattagga ccaggtgtaa tgatggagca atttttaaat gaatctaagg taatagctac
    31201 cttattttgt ggggatgaat tttttgtaaa taatgaaaaa gaagtaacga gtaagatgat
    31261 agctatggta aaaaaattaa accctgatgt agtaatctgt ggaccaagct ttaattacga
    31321 aaacttttct aagatgtcag caatactgtc taaaaatata aatgataaaa cagatatacc
    31381 agcttttgca gctatgtcag aagaaaatat agatgtgata aacgaatata aaaatgacat
    31441 atgtatagtt aagactccca aaaaaggtgg aattggtctt aatgattcat taaataacat
    31501 atgtaaattg gcaaaagcaa tagcgaataa agaagatata actttgatga aagaagaatt
    31561 ttgttattaa taagagtatt aattattagc aagtttaaat attatactat ttataataag
    31621 catacattag taaaatctat ataatttaag gaggggaatt ttaacatgtg gggaatgata
    31681 gcgacatgga gaatggccct agaaggaata actgaggcat cagaacattt agaaaaaggc
    31741 atggaagcag gtgatgctgt agagcttgct gtaagaagag ttgaagacta tccattctat
    31801 aaatcggttg gatatggtgg acttccaaat gaaaattgtg aggtagaact tgacgctgca
    31861 tttatggatg gtaacacatt agatattggc gcagttgctg gtataagaga ttattctaat
    31921 cctgtcagca tagctaggaa gttaagttat gagaaggtaa ataatttttt agttggaata
    31981 ggtgctgaag attatgctca taaaaatgga tttgaaagaa aaaatatgtt gactgataga
    32041 gcgaagttac attataaaaa gagaaaaaaa gagactttag acaaaggatt aagcccatat
    32101 gctggtcatg atactgttgg tatgatatct ttagacaaaa ctggaaaaat gtgtgcagct
    32161 acatcaacaa gtggattgtt tatgaagaaa agaggtagaa taggagattc tgcactatct
    32221 ggttcaggat tctatgttga tagtagtata ggaggagcta cagctactgg tttgggagaa
    32281 gatttaatga agggatgtat atcttatgag atagttaggc ttatgaaaga aggaaagcat
    32341 ccacaagaag catgtgatat tgcagtaaat gaacttgata aacagcttat caaaagaaga
    32401 ggaaaagctg gtgatttatc attagttgcc atgaataata aaggtgaatg gggagtagct
    32461 acaaatatag ttgatttcac tttttcagta gtaactaaag aagaatctcc tgctgtatat
    32521 atttcaaata gagtagatgg aaagactata cataaggtta tatcaaatga gtggatggaa
    32581 gcatatgagg ctagaataaa agcaccgata gaagtaagtg agtaaagtag tgttacataa
    32641 ttaaaataga gttttataat aaggagattt ttatgaatat cataagtaat aaaatagatg
    32701 agttaaaaga agatttatta agtgatatta tagatattgt aaaaatacct agtgtcaaag
    32761 gggagtcaga aaatggcttc ccttttggcg aaaaagtagg ggaggcactt aataaagcat
    32821 tagaaatatc aaaaaaattg ggatttaagg ttagaaactt ggataattat attggttatg
    32881 cagagcatgg agatagtgat gattatgttt gtgtaattgg acatgtagat gttgtacatg
    32941 aaggagatgg ttggaagcat cagccttata agggagaaga aactaatgga agaatttatg
    33001 gacgaggagt actagacaat aaaggaccta taatgtctgc cttatatgga ttatatgcta
    33061 ttaaagaatt aaacttaaaa ttagataaga gagttagaat aatatttggt acaaatgaag
    33121 aaagtggatt tgaagatata ccatattatt tagaaaaaga aaaaacacct attatgggat
    33181 ttacaccaga ttgtaaatat cctgttgtat atggtgaaaa aggcatggca aagattagaa
    33241 taaaaagtaa aatcaattat gaagaagatg tatacctagg ttttatagaa aatatgtctg
    33301 aaaatgtgct tgttacatat aaagagctga atatagaaaa tagtaatact atattagata
    33361 taaaggtaaa atacgatttt agttataaat tgaaagatgt attagatgaa ataaaagcta
    33421 gttttccaaa tagtatcgat atagaggtaa tttctaattt taatcctgta tattttgata
    33481 aagagagtaa tcttgttaaa aagcttcagc tagcttatga aagggttaca tcattagatg
    33541 gaactcccgt gacaactaat ggaggtacgt atgcaaaagt aatgccaaat atagttccgt
    33601 ttggaccttc atttccagga caaaaaggaa tagctcataa tcctgatgaa tacatggata
    33661 tagaagatat aatattaaat gcaaagatat ttgcaaatgc aatatatgaa ttagcaaagg
    33721 agtagttatg ttagaaataa taggaatgtc agtagaagac gctaaaatta tagaagattg
    33781 tggtgcagat agaatagagt tagttagtgc tttaactgaa ggtggattga caccaagttt
    33841 tggtcttata gaaagtgttg ttaacagtgt aaagatacct gtgaacgtaa tgataagaca
    33901 tcatgcgaaa agttttgtat acagtaaaga agatataagt ataatgcaaa aagatatagg
    33961 tgtggttaaa gagattggtg ctaatggagt tgtatttggt gtattggata aaaacaataa
    34021 tatagatgag aaaaatctaa atgttttatt gaaatgttgt gataatcttg atgttacatt
    34081 tcatagagcc atagatgaat caaatacaat agatagtgtt aaaattttga aggattatga
    34141 taaaattacg aatatattaa catcaggagg gaaaggaagt atagttgata acatacaaat
    34201 gataaagaat atgatgctta gttctaatca cataaaaatt cttttaggag gaggtcttaa
    34261 ttttaataat atagaaaaga taaaggaatt aactaaagca agtaattttc attttggtac
    34321 agcgattaga ataaataaca gtccttttga agatatagat agacaaaagc ttaaacaact
    34381 cgtaaatata atttcaagat agtatatgaa tataaaatac ttagtttaaa taaagagagt
    34441 aagttaatta taatttgggt atatgaatgt taataggtat taaatatgaa attattgttg
    34501 agtatgcttt ataactttta agtagtttta aatataaata atgaaagaaa agatagatta
    34561 tggaggtttt agagggttta tagttattaa aaaattataa aggtatattg gcatataata
    34621 cacataattt cctaaaatta ccatcttatg acatgaattc acatattaat aaacaattaa
    34681 tggagatttt tgtggataga aataggagta aaaatagctt taaataaaca aaaacaagag
    34741 ttattaacag ttttatccac attatccaca ggtgtttgta ataatattaa tccacataag
    34801 tatcaatgta actaaaaatg ataaataatt ctatcaattg aatagaaaaa atcataaaag
    34861 taaataataa aaagaggatt atgagagttc gtgtagaata tattattaaa gtataaattt
    34921 tcttatactg acataattga agggagatgt atttatgaac ataattatat ctggaaaaca
    34981 aatcgaatta actgatgcaa taaaaaataa aatcgaaact aaactaagta agttagataa
    35041 ttacattcat ccagacactg atgtaaaagt tacagtaagt gcgagaaaag caagacaaaa
    35101 aatagaagta acaatagcta ctgtaaatgg tcctataatt agagcagaag atatgcagga
    35161 aaatttatac acagctatag atgttgtata tgataaatta agtaaacaat taaaaaaata
    35221 taaaaagaga ttacaagata aacataaaga caacaagagt ataagattcc aagattcaga
    35281 gataaatcta gctgatgaac aagaggatta tggggatgaa aatcaagaat tagtaattca
    35341 aagacgtaag aaatttagtg ttaagccaat gagtgaagaa gaagctgtat tacagatgga
    35401 attaatagga catgattttt atatgttcaa gaacatagat tcagatgaaa tagcagtagt
    35461 ttataagcgt aataatggtg gatatggtat aatagaacat gaataaagca atttagattt
    35521 aatcatatta gctatcgata aataaattat cgatagcttt ttaaattaat attaaaagta
    35581 tagtttttat ggtaaaatga ataatagtac aataaagaac gaggagagat gtaaaatatg
    35641 agctttatgg ataatttatt caacatggca gataaaaaag aattaaaaaa gttcaataaa
    35701 actgttgata taatagattc attagaacct aaatttgaat ctatggcaga ctcggaactt
    35761 aaaaatatga caaatatatt taaagaaaga ttagcaaatg gtgaaagtat agatgatata
    35821 cttccagaag cttttgcagt agttagagaa gtttctaaga gagtgttagg acttagacac
    35881 tacagagttc aaatgattgg aggtatagta ctacatcaag gaagaatagc agaaatgaaa
    35941 acaggtgaag gtaaaacttt ggttgcaact gctcctgttt atttgaatgc gcttactgga
    36001 aaaggtgtac atgttgtaac agtaaatgat taccttgcaa aacgtgatag agaccaaatg
    36061 gcaaaaatct atgagttttt aggaatgagt gtaggcgtta ttatacacgg tcaaaatcca
    36121 aaagttagaa aagaacaata tgattgtgat ataacttatg gtacaaataa tgaatatgga
    36181 tttgactatc taaaggacaa tatggtaata cacaaagagc aaagagttca aagaggatta
    36241 aattatgcta tagtggatga ggttgactct atactaatag atgaggctag aacaccactt
    36301 atcatatctg gacctggaga caaatcaact cacttatatt cagatgcaaa cacatttgtt
    36361 ttaaccttaa aaccagatga ttatgaatta gaagaaaaag ataaggctgt atctctaaca
    36421 gcatcgggta tacaaaaagc agaagtgtat tttaatgttg ataatataac agatatatct
    36481 catacagaat tataccatca tataaatcaa gctttaagag cacatgtaat aatgaagaaa
    36541 gatgttgatt atgttgctaa agatggagaa atagtaatag tagatgagtt tacaggtaga
    36601 cttatgtttg gtagaagata ttcagaaggt ttacatcaag caatagaggc taaagaaggt
    36661 cttaaaatac aaagagaatc taaaacacta gctactgtaa cattccaaaa ctactttaga
    36721 atgtataaaa aactttctgg tatgactggt acagcaaaaa cagaagaaga agagtttaaa
    36781 gcaatttata aaatggacgt tttccaagta cctaccaata agcttatggt aagagaagat
    36841 ttaccagatt ctgtatacaa aagtgaaata ggtaaattta atgctgtagc acaagaaata
    36901 attgaaagac ataaagttaa tcagccaata cttgtaggta cagtatcgat agaaaaatca
    36961 gaattattat cacaaatatt aaagaaaaaa ggcattaaac atgaagtatt aaatgctaag
    37021 catcatgata aagaggcaga gataatagca caggctggta gattaggcgc tgttacaata
    37081 gctacaaata tggctggccg tggtacagat atagttttag gtggaaaccc agatttctta
    37141 actaagagag aaatgagaag aaatggattt aaagaggaaa tagtaaatag ggtagatact
    37201 ccaatagaag gtatacctgt aaaaggtaat gaaattctat ttgaagctag agaagagtat
    37261 gaaaaactat ttgaaaagtt taagcaacaa acacaagaag aacaaaaaca agttgtagaa
    37321 gcaggtggac ttgctataat aggtacagaa agacacgaat ctcgaagaat agataaccag
    37381 ttgagaggtc gtgctggtcg tcaaggtgac ccagggagtt caagattcta tataggtctt
    37441 gatgatgacc ttatgagatt gtttggtagt gatagaatat ctggtattgt agataaaata
    37501 ggtcttgaag aagacatgcc gatagaacat agaatattat ctaagtctat tgaaggagca
    37561 caaaagaaag tcgaaggtaa aaactttgga ataagaaagc atgtacttca atatgatgat
    37621 gttatgaata aacaaagaga aataatatat gcagagagaa aacgtgtatt agaaggtgaa
    37681 gatttacaag aacaaataca atcaatgact cattctataa tagaagaagc agttactctt
    37741 tatacacaag ataaaggatt tgatgaagaa ggattcaaag aacatatgta taatctgttc
    37801 ttgccaaaag gtagcataga aataccagaa atagaaaaac ttaatccagt tgaaataaca
    37861 gaaaaagttt atgaaatagc tatgaagata tatacttcaa aagaagaaca agttggctat
    37921 gagagaatga gagaagttga gagagtaatc ttacttcaag ctgttgataa ccattggata
    37981 gaccatatag atgctatgga ccaattacgc caaggtattg gtcttcgtgc tgtaggtcag
    38041 caagacccag ttattgctta taaaatggaa ggttttgata tgtttgatga aatgaataaa
    38101 catatcaaag aagacactgt aagatattta tttaatataa caatagaaac tccagtggaa
    38161 agaaaagcag ttgtagatgt tgaaaatcta tcttctccat cagatggaac acttccaaca
    38221 agtaaaactg ttaaaaaaga tgaaaaggtt ggaagaaatg acttatgccc ttgtggaagt
    38281 ggtaaaaaat ataaaaactg ttgtggaaga taatctcgga ggattaaatg ttaaagttac
    38341 aagagtatag acatagtatg gagcagatgg cttcaaattt agaagaattg agggtttctc
    38401 tttgacatag cttcattgat gaaaaatata caaattaatg aagaaaagat gaatcaacaa
    38461 gacttttgga atgataatga agtcgctcaa agagtacttc aagaaaataa atctttaaaa
    38521 gagacattag aagaatatga aagcttaaaa agcttattag aagatataga agtattaatt
    38581 gaaataggtt tagaagaaga tgatgattct gtagaaagag atatagaaaa gtccatagaa
    38641 tcaatggaag aaaaattatc tgaaatgaaa ataaagactc ttctaaatgg agagtatgat
    38701 aaaaataatg ctatattatc tataaatgct ggtactggtg gcttagatgc tcaagattgg
    38761 gctcaaatgc ttttgagaat gtatataaga tggtcagaaa gtaagggtta taaagttaag
    38821 cttttagata taatatcaga ccctgaagca gggataaaga cagctacaat tcttgtagaa
    38881 ggaacaaatg cttatgggta tttaaagagt gaaaaaggag ttcatagatt agtgagaata
    38941 tctccatttg acccatcagg gaaaagacat acttcatttg catctataga tgtaacacca
    39001 gagttagatg aaaatataga agtagaaatt aatccgtcag atttaaagat agacacatat
    39061 agagcttctg gagctggagg gcaacatgta aatactactg attcagcagt tagaataaca
    39121 catatcccta ctggtgtagt agttcagtgc caaaatgaaa ggtctcagca tctaaataag
    39181 gacagagcta tgagactttt aatggcgaaa ttgatagaat taaaagagtt agagcaaaag
    39241 gaaaaaatag aagatataca aggcaaatac tcacaaataa cttggggtag tcaaattagg
    39301 tcatatgttt ttcaaccata taaattggtt aaggaccata gaacaaatgc tgagtttgga
    39361 aatgtagata gtgtaatgaa tggaaatata gacttgttta ttaatgaata cttaaaaatg
    39421 aataaaatag tttaataaat atgcactgta attaagatat atgtataaga aggttagtgc
    39481 ttaatatgca cttacagcac ttaggctgag gacgataacc ctcagccttc gttctgtttt
    39541 gttatactta tgaaaaacta acattaggag tgaataaatt ggatataaat caaatcttaa
    39601 aaaaagagtt taatcttaga gatgaacaaa taaataatac attaaaactt attgatgaag
    39661 gaaatacaat accatttata gctagatata gaaaagaaat gactggcgaa atgagtgatg
    39721 ttacactaag agaattttat gaaaaattaa tgtatttgag aaatcttcaa agtagaaaag
    39781 atgatgtagt tagactaata gatgaacaag ggaaattaac agatgaaata acacaaaata
    39841 tagaaaaagc aaaaacatta caagaagtag aagatatata tgctccatat aaacaaaaga
    39901 aaagaactag agcaacaata gcaaaagaaa aaggattaga aaatttagca ttgtcaatac
    39961 tagagaataa tttagataat atagaaatag aggcaaagaa ttatcttgat gaagagaaag
    40021 aagttctttc tattgaagat gctctaaaag gtgctagaga tataattgca gaactagtct
    40081 cagatgatgc taagattaga aagtatataa gagaattagc tttaagagaa ggtatgatag
    40141 ttagcaaaag tgctactgat gagaaatcgg tttatgatat gtattatgat tataacgaag
    40201 cagtaaaaag tatggctcca catagagttt tggcaataaa cagaggagaa aaagaaagct
    40261 tcctaaaggt taagttagaa ataaataatg ataaagtttt aaattacata ataaacgaat
    40321 atgtaaatga taaaaatttc aaaaataaag aagaaatcgt aagttcaata gaagattctt
    40381 ataaaagatt aattttccca tcaatagaaa gagaaataag aaatcattta acagaaatag
    40441 ctcaagaaag agctataagt gtatttggaa agaatgttaa aagtttattg cttcaaccac
    40501 cagttaaaga caaagtagtt atgggatttg accctgcatt tagaactgga tgtaaaatag
    40561 ctgtagtaga taaaaatggt aaactactag attatacaac agtatatcca acagaccctc
    40621 aaaatgatgt agagggagca aagaaagttt taaaaggatt gattgaaaaa tacgatatag
    40681 atataatttc aattggaaat ggtactgcat ctagagaatc agaaactttt gtatcagaaa
    40741 tgataaaaga aatagatagc gaagtgcagt atgtgatagt tagtgaagca ggagcatctg
    40801 tttattcagc atctgaactt gcaaatgagg aacatccaga tataaatgta tcaataagag
    40861 gagctatatc tatagctaga agattacaag acccattagc agagcttgta aaaatagacc
    40921 ctaaaagtat tggtgttggt caatatcagc atgatttaaa caaaaagaga ttagaagaag
    40981 tgctagatgg agttgttgaa gattctgtaa atagtgttgg agtagattta aataccgcat
    41041 catactccct actagaacat gttgcaggaa taagtaaagc tatagcaaaa aatataatag
    41101 catatagaga agaaaatgga gattttacat caagagcaca attgaaaaaa gtaaaaagac
    41161 taggacctca agcatttact caatgtgctg gatttatgag aatcttagag ggtaaaaatc
    41221 cactagataa tacaggtgtg catcctgaaa gttatgatat atgtaaaaaa atgattgaga
    41281 taattggata ttctttagat gatgtaaaga ataagaatat aggtgaaata gatgaaaaaa
    41341 taaaagaaat tggcttgagg gaattaagtg aaaaactgga agtaggacaa gttacgttaa
    41401 aagatatcat agctgaaatc aaaaaaccag gaagagaccc aagagaagaa ggtataaaac
    41461 ctatacttag aactgatgtc ttaaaaattg aagatataca agagggtatg actttaaaag
    41521 gtacaataag aaatgttgta gactttggtg catttgtaga tattggaata aaaaatgacg
    41581 gattagttca taaatcagaa atgagtaata gttttgtgaa agaccctatg agtattgtta
    41641 cagtaggtga tatagtagat gtaaaagtca ttggaattga cttaaacaaa aaaagagttg
    41701 ccttatcaat gaaaaaatag ataagtttat gtatggttat agtacttaaa ttctatattt
    41761 gtaaatgaga ttactcgagt gtataaatgt taaaaatttg taaaagtata tagtaagaaa
    41821 atatattaaa ataagaacga tagtcttgaa aatcaggagg aaaatgaatg atttttataa
    41881 aaaatggtaa aataaatacc ataacaaatg ggataattca tggagatata cttatagatg
    41941 aaggaaaaat aatagaaatt ggagaagatt tgatagctcc tttagatgta gaggtaatag
    42001 atgctagtaa taaacttgtg tttcctggat ttatagatgc tcatactcac ttaggtttat
    42061 gggaagatgg tatagggttt gaaggagcag atggaaatga agagactgac ccaataacac
    42121 cacaattaaa tcctatagat ggaattaatc caatggatag aacttttaaa gaagcttttg
    42181 aaggtggaat aacttctgtt tgtacaaccc ctggaagtgc aaatgttatg ggtggtcaat
    42241 gtatagctat aaagacatgt ggaaaaagaa ttgataaaat ggtaataaaa aatccagttg
    42301 catctaaaat tgcttttgga gaaaatccaa agagctgtta tggtcaggat gataaatctc
    42361 ctcaaactag aatggctata gctgctttac ttagagaaaa cctaaaaaaa gcagaagaat
    42421 atttagagga tattgatatg tatgagagtc atgatgatga agactgtgaa aaacctgaat
    42481 atgatataaa aatggaaagt ttaataccag ttttaagaag agaaattcct tttaaggcac
    42541 atgctcatag agcagatgat atgtttacag caataagaat tgcaaaagaa tttaatttaa
    42601 agcttacatt agaccattgt acagaaggtc atcttattgt agatgaatta gtggaagaag
    42661 ggtttcctgt aatagtaggg ccatctttgt cagaaaggtc taaatttgag cttagaaatt
    42721 tgacatttaa tacagcagga atactttcaa atgcaggtct tgatgtatgt ataatgacag
    42781 accatccagt aataccagtt caatatcttc caatatgtgc aggtattgca gtaaaacatg
    42841 gtatgaaaga agaaaaagca atagaatcaa ttacgataaa tccagctaag actttaggaa
    42901 tagaagatag agtaggctct atagaagttg gtaaagatgc agacctagta atttgggata
    42961 attctccact agagattcaa agtaatgtac tttacactat aataaatggc aaagtagtgt
    43021 atgagaaaaa ataacttgac aattattaaa aaattaacta atataagagt gtaaggaaaa
    43081 ttaaaaaatc aaagaagatc ttcggggcag ggtgaaattc cctaccggcg gtatagcccg
    43141 cgaactactt aggtggttga tttggtgaga ttccaaagcc gacagtaaag tctggatgag
    43201 agaagatatt tatggaagat aaataagttt attttagttt gtttaaacta tatccataaa
    43261 aactcgaaga tagggtcttc gagttttttg tttttcctaa aaaatataaa catattgatt
    43321 agggggaaaa taattatgca aaacacagca aaaagaggca atatgatttc aacaagaact
    43381 ttagtagtaa tgtcaatttt atctgcaata tcttatttat taatgtttat tcaagcacca
    43441 ataccaggta tatttccaga tttcttaaaa attgacttat cagatattcc agcaatattc
    43501 ggaggaatgt ctctaggacc attagttgga tttggaatag ttgtagtaaa aaatgtgtta
    43561 caagcaataa cagcaacaac aactggagga ataggagaat ttgcaaatat attaattggt
    43621 ggttcttatg taatgatact ttgttattca tataagagaa gtggagattt aaaaggtgta
    43681 ctgattggtt ttgtattagg aacaatatct atgactataa ttggttcatt agtaaattat
    43741 tttgtaatgt tgccacttta tggtcaactt atgggactag atgctataat aggtttaggt
    43801 tcagctataa atcctcaagt tcatgattta ttcacttttg taatttggat gatagctcca
    43861 tttaatgtgc tgaaggctgt tataatatct ttagtaacat tgccacttta taaaaaaatg
    43921 ggaaatataa taaagaaata attagacaag aaaatataaa attaaatgta ttgaatagcc
    43981 ataatttatt tataacttca gtcctatatt gtgattgata tattaaatat attatggctt
    44041 tattaattta tcaaaaaatg tatataaaat aagaaatatt tagacaaatt taaattttaa
    44101 aataaaggaa gaattaaaac gattaactat taaaatatga aaaaggtatc tacaatatac
    44161 agagcgaatt tataataaac aaaaaataaa gttattatta aaaaactata aagactaata
    44221 atcaaacttt taataataac taatttaata atgatagggg tgtatattca tgcatttatc
    44281 taaaaaaata aaaaaattat ttttagatga taaagggtat tattttaata gtaattggga
    44341 agatattcat attggtattt atgatacaaa attaggagat tctttaaagt atatcactaa
    44401 tgaatatatt gaaaatatgg aaagggtaaa atacgattat cgtgaattaa aaaatataga
    44461 aaatcaaact gatggtatgt gtattttagc aataaggcag aattacaagg cattagagta
    44521 tgtaaaaaat caaaccgaag aattatgttt agaggcaata aagtataatt ataaagcact
    44581 tgaatatgta aaagaacaaa cagaatactt gtgtcttgaa gctataaaaa aagattgtaa
    44641 tgcacttaag tatgtacgaa ataaaacaga agggcttatt ataaaagcaa taagtcattc
    44701 ttccaatata gatgttgtaa gtatactaaa ggctttagag actcaaacaa gaaggatatg
    44761 tttagaagca attaaaaaag atggcaggtg tcttgcatac gttagagaac agagtgaaga
    44821 attatgtata gaggccatta agcaaaatta taaagcatta aaatatgtaa aaaatcaaac
    44881 ggaaaaaatg tgtatagaat ccgtaagaca aaatggtatg gcattacaat atgtaaataa
    44941 acaaactgat aaaatatgta tagaagctgt caaacaagat ggaagaagtc tacaatttgt
    45001 caacaataag acagaagaaa tatgtataaa tgctataaga tatttaaata aaaaatataa
    45061 tattaaagat gtactaagtt acatagataa gtatacagaa gatatatgta tagagattgt
    45121 aagacaaaat ggaaaaatgc ttatgtatat aaaaaatcaa acagaaaaaa tgtgtattga
    45181 agcagtaaaa gagaactata agtctcttaa gtatgtaaaa gagcaatctg agagaatatg
    45241 taaagaagca ttgaagcaaa accataaagc aaaagagtat gtaaaaattg ctattgatga
    45301 ttgtatataa ggtgtagata cagccatcaa tagtgaaaga taaaaaaaga taaagacaag
    45361 tactttataa taatctaggc ttatactata attaattaag cagtgatata aattcacatg
    45421 acgattatat caaaatggag atgaattgat ggcaaaaata tatttagaaa atgaaaataa
    45481 aactagagaa ataggatata aattaggtaa acttttaaaa gaaggcagtg ttatctgttt
    45541 agtaggagat ttaggagctg gtaaaactac aatgacacaa agcttggcag attcattagg
    45601 gatagaagat tatataacaa gtcctacttt tactataata aatgaatatg aaggaaaaat
    45661 acctttgtat cattttgatg tttatagaat aggtagtagt gatgaaatgt atgatattgg
    45721 ttatgatgaa tatgtaaatt caaatggaat ctgtataata gaatgggcta atctaataga
    45781 agatatactt ccaaaagaat atttgaatat agaattgaga tataaagatg aaggaagaga
    45841 aatgatatta actccaaaag gagagtttta taaagagata gttgaggagt tgattaaata
    45901 atgaaaatac taggtatgga tacgtcttct atggcagcaa gtgtagcagt tgttgaagat
    45961 gataacttaa tatgtgagtt tacagtaaac aacaaaaaaa ctcattcaca aaagcttatg
    46021 ccaatgatag aaaatatgtt gagtatgagt gatttaagta taaaagatat ggatttatta
    46081 gctgtatgta taggtccagg ttcgtttaca ggacttagaa taggtatggc tacagtaaaa
    46141 gctatggccc atgtaaataa tataccaata atagcagtaa attctttgga atctctagca
    46201 aataacataa atttctgtga tagaaagata tgttgtatat tagatgcgca gaaaaatcaa
    46261 gtttattcag ctaaatacaa gtttgaaaat ggagagatga tagaattaga tggtgttgat
    46321 gttgtagagt ttgatgcttt agtagatgaa attgtatcaa ctaatgaaga atttataata
    46381 gttggagaag ctgtatacaa atataaagac aagttagaga atataaaaaa tataaaaata
    46441 ccttcaccag ctaataatgt atctaacgca ggaagcttat gctctttagc attaaataaa
    46501 tataataaaa atatagatgt acatacttgt tatacgataa atcctatgta tatcagaaaa
    46561 tctcaagctg aagtacaata tgatgaaaaa atgaagaggc taaataatgg aagataatac
    46621 aaaactaata gtagatgata taaaaataga agaaatgact acagaagata tagatgaagt
    46681 ttttgaagta gaaaaaaatt gttttgaaga ttattggtca aaagagtctt ttagaaaaga
    46741 gttgtctaat gaagtagcaa ggtatatagt agctaagtta aatggaaaaa ttgtcggata
    46801 tgtgggaatt tggcttatac ttgatgaagg gcatataaat aatgtggctg ttcatagtga
    46861 ctatagaggg aaaaaaatag gggacaagtt gataaaatgc atagttgatt tatgtaaaga
    46921 taataatatt gcatctatga cattagaagt tagagcatca aataaaatag cacaaaattt
    46981 atatagaaaa tatggattta aaatgggtgg aataagaaaa gaatactaca gtgataataa
    47041 agaagatgca attataatgt ggaaccaatt aaaagaggtg tagagtttat gagtgatata
    47101 ataacattag ctatagagag tagttgtgac gaaacagctg catcagtttt aaaaaatggt
    47161 agagaagtac tttcaaacat aatctctact caaatcgaga cacataaaaa atttggtggt
    47221 gtagttccag aagtggcctc aagaaaacat gtggaaaata ttgatatagt tgttcaagaa
    47281 gctttagaca aagcaaatat aggttttaat gatatagacc atatcgcagt tacatatgga
    47341 cctgggctag taggtgcttt attggttggt ctatcttatg ctaaagcact tgcatatact
    47401 ttgaatattc cacttgtagg agtaaatcat atagaagggc atctaagtgc aaactatata
    47461 gagcataagg atttaaaacc tccttttata acattaatag tatctggagg tcatactcac
    47521 ttggttgaag taaaagatta tggcaagtat gaaattttag gaaaaacaag agatgatgca
    47581 tctggagaag catttgataa gatttcaaga gctatgaatt taggctatcc aggaggtcct
    47641 attattgata atctggccaa gaatggaaat aaacatgcta tagaatttcc aagagcctat
    47701 ttagaagaag atagctatga ttttagtttt agtggattaa aatcttctgt attaaattat
    47761 ctaaatggaa aaagaatgaa aaatgaagaa atagtagttg aagatgtagc agcatctttc
    47821 caagaggctg tagtagaggt tttatctaat aaagcactaa aagcagtaaa agacaaagga
    47881 tacaatatca taactctttc tggtggggtt gcttctaatt cggggcttag agctaaaata
    47941 acagagcttg ctaaagacaa tggtattact gttaagtatc ctcctctaat tttatgcaca
    48001 gataatgcag ctatgatagg gtgtgcagga tattataatt ttataaatgg gaaaacgcat
    48061 gatatgagtt taaatgcagt tccaaattta aagataaatc agtagagtta gatgaatttg
    48121 taaatataaa agtattgttt caaaaaagta agtagaagtg aaattaaata taataaaaat
    48181 aaatatagta tatattaaaa cttgaagtaa aactatttat aatctttata ttccaatgaa
    48241 ttgcaacaaa ttttaggata tacagattgt ctttggctta gatttgcttc aagtttaatt
    48301 ttttctatag aaagattatt ttaaaaagtg ggtaatatac cctttttttt cattcttaaa
    48361 aagttttata ctaattatag aaagattaag gaggggaaga aatgagtcaa agtaaagaag
    48421 acaaaataag aagtattttg gaagcaaaaa atattaaatc aaattttcaa aataaagaaa
    48481 atttaagtga atttaatgaa aaaaaagcaa gtaaaagagc agaagactta ttagatgtat
    48541 attataatac attatctact gctgatatgg aatttccata ttggtataat agagaatata
    48601 gaaaatcaga tggtgatata ccagtagtta gaagagctaa agctcttaaa gctgcttttt
    48661 cacatatgac accaaatata atacctggtg agaaaatagt aatgcaaaag acacgccatt
    48721 atagaggttc ttttccaatg ccatgggtaa gtgaaagttt ctttgttgct caaggagaac
    48781 aaatgagaga agaggcaaag aaattagcca gtaacacagc tgatgaactt acaaaatttg
    48841 ggtctggtgg aggaaatgtt actgaaagtt ttggaaatgt cgtttcaata gctggtaaat
    48901 tcggaatgag aaaagaagaa gtacctgtac ttgtaaaaat ggcaaaggaa tgggttggaa
    48961 agtctgttga agatttaggt tttcactatg aaaaaatgat gccagattat gatttaaaag
    49021 aaaacctaat gagtacactt atatgcatgt ttgattcagg gtatacactt cctcaaggta
    49081 gagaagttat aaattatttt tatcctttaa attatggatt agatggaatt atagaaatgg
    49141 caaaagaatg taaaaaggca gttgcaggaa atgcatctgg agatgggctt attggtatgg
    49201 accgtctata tttttatgag gctgttattc aagttattga aggattacaa acttggattt
    49261 taaattatgc aaaacatgca aaatatttag aaagtataga gacagattta gaagctaaaa
    49321 aagaatactc agatttagtg gagatattag aacatattgc acataaacaa cctcgtacat
    49381 ttagggaagc tcttcagttg acttatacaa ttcatatagc atcagtaaat gaggatgcta
    49441 tatctggtat gtctatagga cgttttggtc aaattttata tccttggtat gaacaagata
    49501 tagaaaaagg actaattaca aaagaagagg ttatcgaatt attagagctt tatagaataa
    49561 aaatcacttg tattgactgt tttgcttcag cgggcgtaaa tggaggagta ctatcaggta
    49621 atacatttaa tactttatct ataggtggat taaaagaaga tggttctaca ggagctaatg
    49681 aactagaaga gttattatta gaagcaagta tgagatgtag aactcctcaa cctagtttaa
    49741 ctatgctata tgatgaaaag cttccagaag atttcttaat gaaagctgct gaatgtacaa
    49801 agcttggttc tggttatcca gcctgggtaa acaactcaaa tggaacaaca tttatgatga
    49861 aacaatttgc tgatgaaggt atgactgttg aagaagcaag agcatttgca ttaggtggat
    49921 gtttggaaac gtctccaggg tgttggaaac agttaacttt aaacggaaaa acgtactcta
    49981 ttgcaggtgg agcaggtcaa tctgctggct ctggagttca ctttatagct aatcccaaaa
    50041 ttttagaact tgttcttatg aatggtaaag accatcgtat gaatatacaa gtatttgagc
    50101 ctcataataa gccattagat acttatgaag aagtcataga agtatttaaa gattattata
    50161 aacaagcaat aaatgtttta gaaagagcaa ataacattga gttagatata tggagaaaat
    50221 ttgatacttc tataataaat tcactactaa agccagactg tttagacaaa ggtcaacata
    50281 taggaaatat gggatatcgt tataatgcaa cattaaacgt agaaacttgt ggtactgtta
    50341 ctatggtcaa ttcatttgca gcacttaaga aacttgtata tgatgacaaa gcatttacaa
    50401 tagaggaaat gaaggatgca atattaaata attttggatt taaagatgca ttagaagtag
    50461 gaaattattc tatggcagac caagtaaaag tagataagac tggtaaatat gatgctattt
    50521 acaaggcctg tcttgatgca cctaaatatg gaaataatga tttgtatgca gataatattc
    50581 ttaaaaacta tgaggtatgg ttatcaaaag tatgtgaaga agcacaatct ttatatgcta
    50641 agaagatgta cccttgccaa atatctgttt ctacacatgg accacaggga gctgctacct
    50701 tggctactcc agatggtaga ctaagtggaa caacatattc agatggttca gtctctgcat
    50761 atgcaggaac agataaaaat ggagtatatg cattatttga atctgcaact atttgggacc
    50821 aagcagtagt tcaaaattct cagatgaatt taaaacttca tccaactact ataaaaggac
    50881 aacaaggaac taaaaaatta ttagatttaa caagaagtta tttaagaaaa ggtgggttcc
    50941 atattcaata taatgttgta gattcagaaa cattaaaaga tgcacaaaaa aatcctgaca
    51001 attatcgtca attaatggta cgtgttgctg ggtttactca atattggtgt gaattaggta
    51061 aaccaataca agatgaagta attgctagaa cagagtatga aggggtgtaa aatatgagaa
    51121 agcatagtga ttgtatgaat ttttgtgcag tagatgcaac caaaggaatt tgtagattat
    51181 caaaacaaat gattaattta gatgattcag catgtccaga gataaaagta atgccaaaat
    51241 gtaaaaattg taaaaatttt gttgaagcta atgatgaagg tataggcaaa tgtgttggcc
    51301 tagagaaaga agactgggta tattcaacat tgaatgcaat tacttgtgaa ggacatgtgt
    51361 ttaatgagta gtcaaaagca attagaaggc atgattttcg atgtacaaag cttttcagtt
    51421 catgatggtc caggttgtag aacaactgta tttttaaatg gatgtccact gagttgtaaa
    51481 tggtgtgcaa atccagaaag ttggactgta agaccacata tgatgtttag tgaattatct
    51541 tgtcaatatg aaaatggatg tactgtatgt catggtaagt gtaaaaatgg tgcattgagc
    51601 tttaatcttg ataataagcc agttatagat tggaacatct gtaaggactg tgaaagtttt
    51661 gaatgcgtca actcatgtta ttataatgcg tttaaattat gtgcaaaacc ttatactgta
    51721 gatgaacttg tgcaggtaat taaacgtgat tctaacaatt ggagaagtaa tggaggagta
    51781 acatttagtg gaggtgagcc actacttcag catgaatttt tacatgaagt attattaaag
    51841 tgtcatgagg taaatataca cactgccatt gaaacaagtg catgtgtatc aaatgaagtt
    51901 tttaataaaa tatttaaaga tattgatttt gcatttattg atataaagca tatggataga
    51961 gaaaaacaca aagaacaaac aggtgtatat aatgatttaa tattagaaaa tatatcaaac
    52021 cttgcaaatt ctgactggaa tggaaggtta gtacttagag taccagttat ctctgggttt
    52081 aatgatagtg ctgaaaatat aagtgatatt atatctttta tgcacaaaaa taatttaata
    52141 gagattaact tattaccatt tcataggcta ggagaatcaa agtggattca attaggtaag
    52201 gagtatgagt attctgataa gggagatatt gatgaagagc atcttgaaga attacaagat
    52261 atatttttgg ataatggtat agcttgctat gttggtcatg agacagcttt ttaaaatcaa
    52321 atacaagttt ttttaattaa aaaaggatgt tacactaaaa gctagagtta catccttttt
    52381 tatgtggttt ttatgtagaa ataattaagt aaagttttat atgttttata tattgttcat
    52441 attgctttat gtccataaat attactttaa atttatactt tatatatttt ttatagttta
    52501 aattacagtt agagaatata ttacgtttac tttattgaag cagaagtttt gtggtaaaat
    52561 actactatga agaaatttaa tagaatatat tttagaaaca actaaaaata atagttagga
    52621 ggaagacatc tatatgaaca aatcatggag taacttgatt attaagaata aagacaaaaa
    52681 agtacgttca gggtggatta ttataactgt tatggctgcc ttttacatat ttgagtatta
    52741 tttaagcact atacttatgg aaataataag aaaaattctg attgctacag gtgacattaa
    52801 tttaaaaaca aactattttt cggaatatgc taattggtta aatgacgttg ggttacaggt
    52861 tgtatttcaa attctaacag attcacttat gattattatt cctataattg tatggagatt
    52921 tattatgaaa aatcctatta gtgaaatggg attacattct tatagaatta agaaaaaaga
    52981 aggatatgta ggaatgttat taggaatatt taattgtaca gtaatcttta ttataacaat
    53041 aacaattggt ggtggtcatg ttacttcatg ggtaccaaaa atttcacctt taacagtttc
    53101 ttggatactt gtctttattt tagttgcttt tgcagaagaa attttaaatc gtggtttttt
    53161 tatggcagtt ttacgaagat gtaaaaatat ttattttata atgattgttc catctgttat
    53221 ttttgggttg attcatatat ggaatcctga tgttactttt ttgtctgtaa ttaatattat
    53281 aattattggg attctttttt catatatgtt tataaagtcg tcgaatatat ggatgtgtat
    53341 aggttatcat tttacatgga atgtatttca ggggattata tatggtatgc ctgtaagtgg
    53401 acttcaggtt ccagggatta taactacaca aatcactcat ggtaatctat taaatggagg
    53461 gatgtttggt attgaaggtg gaattttgac tacttttgtt aatctactta gctttatatt
    53521 tgtatggtat tactatagag attctaagta tgattttgtt agtgataaag taaatgtgac
    53581 taatatcaag aattagttga ttataataaa ttttaataat atcttctaaa cttaaagtat
    53641 tctgtaggat tgttggaaat ttacaaaaat ataaaaataa cttggatatt ctatttactc
    53701 caagttattt ttatattctt ttgtaaatag aaatggaaca taaaaaatta aaacaaccat
    53761 agatactatt agtatatcat ggtaagtgat agttgattct ttagaatttg tgataagaaa
    53821 agcactatta tttaaaacat gaattatagc acaaaaccag atattcttag tttttatata
    53881 aagatatcct aaaaatattg aaattccaac tatatgtatt gaacgtaata ttactcctaa
    53941 tattggcgct tcaggtgtat ataatgtaaa tattagtggt aagtgccaca agctccaaca
    54001 catacctaat attatgactc ctaatttttt accaaattta tcaaaaaaaa tattctgtaa
    54061 aaaacctctc caggcatatt cttcactaaa aaaaatataa atagtagtta atgatgttat
    54121 tggagtaaat ataaatttta tgataccact ataattaggt tgagcccctt gtagtattag
    54181 tataaatgat gttggaacaa ttacagaaat cataaagtaa ataattaata aagaacctag
    54241 tttaatattt tttacaggat ataaattttt tttatttgat gaatatatta taattgataa
    54301 gctacttata aaagttaata t
//
LOCUS       DAGQWR010000006        49274 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.006, whole genome shotgun sequence.
ACCESSION   DAGQWR010000006 DAGQWR010000000
VERSION     DAGQWR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.006"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(19..3447)
                     /gene="uvrA"
                     /locus_tag="MIW95_000264"
     CDS             complement(19..3447)
                     /gene="uvrA"
                     /locus_tag="MIW95_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBY3149624.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFENELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFAKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKTPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPNKNYTALTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFTETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCNEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            3766..6000
                     /locus_tag="MIW95_000265"
     CDS             3766..6000
                     /locus_tag="MIW95_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY3149625.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMDEILNIDSFNGEGTIEIINSQGKTLFIGKNNELIKDLSYKYKDSADEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDVVLGELNK
                     LNSFTIIMTFITTCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSNFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            6256..8286
                     /locus_tag="MIW95_000266"
     CDS             6256..8286
                     /locus_tag="MIW95_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY3149626.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDLIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENEIILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            8283..8735
                     /locus_tag="MIW95_000267"
     CDS             8283..8735
                     /locus_tag="MIW95_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY3149627.1"
                     /translation="MNKLVNVKCSFKIHANSKEEAIVSLVDVVSKEGYLIDKNQFLED
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDIPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            8737..9792
                     /locus_tag="MIW95_000268"
     CDS             8737..9792
                     /locus_tag="MIW95_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBY3149628.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            9859..10167
                     /locus_tag="MIW95_000269"
     CDS             9859..10167
                     /locus_tag="MIW95_000269"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBY3149629.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            10271..11536
                     /locus_tag="MIW95_000270"
     CDS             10271..11536
                     /locus_tag="MIW95_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBY3149630.1"
                     /translation="MKKLPIKTVVQRLLDLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLSVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVDKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKNEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            11624..12340
                     /locus_tag="MIW95_000271"
     CDS             11624..12340
                     /locus_tag="MIW95_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBY3149631.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(12535..12828)
                     /locus_tag="MIW95_000272"
     CDS             complement(12535..12828)
                     /locus_tag="MIW95_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBY3149632.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(12834..13049)
                     /locus_tag="MIW95_000273"
     CDS             complement(12834..13049)
                     /locus_tag="MIW95_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149633.1"
                     /translation="MRTLSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            complement(13594..14106)
                     /locus_tag="MIW95_000274"
     CDS             complement(13594..14106)
                     /locus_tag="MIW95_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY3149634.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVKQILDLPVKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            14556..15032
                     /gene="purE"
                     /locus_tag="MIW95_000275"
     CDS             14556..15032
                     /gene="purE"
                     /locus_tag="MIW95_000275"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBY3149635.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            15038..15736
                     /locus_tag="MIW95_000276"
     CDS             15038..15736
                     /locus_tag="MIW95_000276"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBY3149636.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            15748..17115
                     /gene="purF"
                     /locus_tag="MIW95_000277"
     CDS             15748..17115
                     /gene="purF"
                     /locus_tag="MIW95_000277"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBY3149637.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            17109..18185
                     /locus_tag="MIW95_000278"
     CDS             17109..18185
                     /locus_tag="MIW95_000278"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBY3149638.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            18179..18772
                     /locus_tag="MIW95_000279"
     CDS             18179..18772
                     /locus_tag="MIW95_000279"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBY3149639.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            18765..20297
                     /gene="purH"
                     /locus_tag="MIW95_000280"
     CDS             18765..20297
                     /gene="purH"
                     /locus_tag="MIW95_000280"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBY3149640.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEVLKQKKNLRILKLAKIEDSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKAKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            20308..21558
                     /gene="purD"
                     /locus_tag="MIW95_000281"
     CDS             20308..21558
                     /gene="purD"
                     /locus_tag="MIW95_000281"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBY3149641.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKVYCAPGNAGITNVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMETILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMLDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            21583..25389
                     /locus_tag="MIW95_000282"
     CDS             21583..25389
                     /locus_tag="MIW95_000282"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBY3149642.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAVPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKTFTDDKLFE
                     ASYGNIVLELANDDLCMLEGYNYVVLGSTTKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERVGA
                     NANIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVFKNIIGEKDQKIFES
                     GVKYFR"
     gene            25595..26467
                     /gene="rfbD"
                     /locus_tag="MIW95_000283"
     CDS             25595..26467
                     /gene="rfbD"
                     /locus_tag="MIW95_000283"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBY3149643.1"
                     /translation="MKILITGSNGQLGKELVSQLEAINQSINQPKYVILATTRNDLDI
                     SNQTNIDNFILHNKPDVIVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LVHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFELKNIDIKVNPIKSNEFKCKAQRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            26576..27457
                     /gene="rfbA"
                     /locus_tag="MIW95_000284"
     CDS             26576..27457
                     /gene="rfbA"
                     /locus_tag="MIW95_000284"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBY3149644.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLKITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDMQYGR"
     gene            27494..28051
                     /gene="rfbC"
                     /locus_tag="MIW95_000285"
     CDS             27494..28051
                     /gene="rfbC"
                     /locus_tag="MIW95_000285"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBY3149645.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFCDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVRYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFNYKCTNYYYPEFEHGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICIRR"
     gene            28089..29072
                     /gene="rfbB"
                     /locus_tag="MIW95_000286"
     CDS             28089..29072
                     /gene="rfbB"
                     /locus_tag="MIW95_000286"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBY3149646.1"
                     /translation="MKKILITGGAGFIGSNFVHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDSSNYKFVKGDISNREQMYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSPINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLDKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            29111..29779
                     /locus_tag="MIW95_000287"
     CDS             29111..29779
                     /locus_tag="MIW95_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBY3149647.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNSSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSENLFDELGSVVNNTASRFIDVDTKDKSVKSRIENAIEQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            29788..30216
                     /locus_tag="MIW95_000288"
     CDS             29788..30216
                     /locus_tag="MIW95_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBY3149648.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            30240..30554
                     /locus_tag="MIW95_000289"
     CDS             30240..30554
                     /locus_tag="MIW95_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBY3149649.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            30706..30981
                     /gene="flgM"
                     /locus_tag="MIW95_000290"
     CDS             30706..30981
                     /gene="flgM"
                     /locus_tag="MIW95_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBY3149650.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGVKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            30986..31402
                     /locus_tag="MIW95_000291"
     CDS             30986..31402
                     /locus_tag="MIW95_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBY3149651.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDRYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTATYNSYGNIRK"
     gene            31420..32730
                     /gene="flgK"
                     /locus_tag="MIW95_000292"
     CDS             31420..32730
                     /gene="flgK"
                     /locus_tag="MIW95_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBY3149652.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETIINEPSDYGISAGIDDFFKGWESLAKDINSDSKKSLVVENASNLATLISESYK
                     KLNKLKGDIDDNINSEIKQINDMLKSLEDLNKSIDVISGSGSTPNDLLDERDRILDDL
                     SFKLDLENSDVKNMLSDGKLELNELKNADGTWKTGVSGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKVNPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            32749..33681
                     /gene="flgL"
                     /locus_tag="MIW95_000293"
     CDS             32749..33681
                     /gene="flgL"
                     /locus_tag="MIW95_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBY3149653.1"
                     /translation="MRVSTGMMSSNYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            33697..34089
                     /locus_tag="MIW95_000294"
     CDS             33697..34089
                     /locus_tag="MIW95_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBY3149654.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            34083..34295
                     /gene="csrA"
                     /locus_tag="MIW95_000295"
     CDS             34083..34295
                     /gene="csrA"
                     /locus_tag="MIW95_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBY3149655.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            34317..34712
                     /gene="fliS"
                     /locus_tag="MIW95_000296"
     CDS             34317..34712
                     /gene="fliS"
                     /locus_tag="MIW95_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBY3149656.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            34712..35068
                     /gene="fliS"
                     /locus_tag="MIW95_000297"
     CDS             34712..35068
                     /gene="fliS"
                     /locus_tag="MIW95_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBY3149657.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELIRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            35089..36612
                     /gene="fliD"
                     /locus_tag="MIW95_000298"
     CDS             35089..36612
                     /gene="fliD"
                     /locus_tag="MIW95_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBY3149658.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLKVIGNDNLAQQIANDNGIT
                     FANDASGNKVASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            36615..36950
                     /gene="fliT"
                     /locus_tag="MIW95_000299"
     CDS             36615..36950
                     /gene="fliT"
                     /locus_tag="MIW95_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBY3149659.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISSKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            37052..37924
                     /locus_tag="MIW95_000300"
     CDS             37052..37924
                     /locus_tag="MIW95_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBY3149660.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTTN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTDAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            38017..40158
                     /locus_tag="MIW95_000301"
     CDS             38017..40158
                     /locus_tag="MIW95_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBY3149661.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRSSIESELIIVDTGSTDD
                     TINISRKYTENVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNIDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFEELKENDNSPYTNYQLGKNFIILGKYHEAL
                     YYLEKSYELYSKLESAPGYLITSLVKTYLYLGKHKKCEKLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYESVISEYNNIEDMEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YKNIEEVINNIKDDEKEHVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKVAFEDKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDI
                     KKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYRNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNDANEKLEYIHSMRKLLNEYPFYNKIIKILISELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKSLVDDYSKTFMDDVKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            40182..42059
                     /locus_tag="MIW95_000302"
     CDS             40182..42059
                     /locus_tag="MIW95_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBY3149662.1"
                     /translation="MNTPIVVNEKSKDIIKDWQYTYTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFMFAHKINTSNINLWKYRLKLVGFCMLIKKSVLDNVGVLDELFTPGNFEDDDLSFR
                     MLESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIADIEKVKLDYEKDFFDYIILGDVLEHLTDPWKIVNELKQYLKKDGCLISSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSVTATSMPLSEID
                     NRFIDTMCKESCKEMRQQYESYQYIVKATKSIDTKRYSSISMVNLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLVDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSKKVQSESDIQDILYFIEGKRY
                     E"
     gene            42052..44166
                     /locus_tag="MIW95_000303"
     CDS             42052..44166
                     /locus_tag="MIW95_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBY3149663.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDAFIINKNFIYDILEIFKKNTDVGMIGVAGVKDI
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVANINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLDIVSKIAHFS
                     VFNHTGLYSCEKCERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAIDDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKEKQSEIKHFVIDKHCIEIKNYIDKIYKEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            44175..45170
                     /locus_tag="MIW95_000304"
     CDS             44175..45170
                     /locus_tag="MIW95_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBY3149664.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIIYKGEEILAVVPACETYENGERVFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLETYLQEQSFNKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKK
                     DILANFRYSKRRYFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            45262..46494
                     /locus_tag="MIW95_000305"
     CDS             45262..46494
                     /locus_tag="MIW95_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBY3149665.1"
                     /translation="MKRLMILGSIKYFENIVMSAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEIFDENNENMIENF"
     gene            46514..47458
                     /locus_tag="MIW95_000306"
     CDS             46514..47458
                     /locus_tag="MIW95_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBY3149666.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSDIFLYNMKDAKLKAILDGNYITA
                     FRTAAVAGLAIDTFAKKDFNTISIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFIKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYNVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLNCEEIELKEPKEKYWI"
     gene            47552..47959
                     /locus_tag="MIW95_000307"
     CDS             47552..47959
                     /locus_tag="MIW95_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HBY3149667.1"
                     /translation="MDKYNLIKFLSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKESIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            48046..49137
                     /locus_tag="MIW95_000308"
     CDS             48046..49137
                     /locus_tag="MIW95_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBY3149668.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVRHFEEE
                     FASYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASKMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFVGMEDEEVSYIIKIINEY"
BASE COUNT        19545 a         5194 c         8751 g        15784 t
ORIGIN      
        1 atgtgtattt aattgttttt atataaaatg tccagtaata ctatttggat ttatttttat
       61 ttcatcagga gttccagaag caacaatttc ccctccattt attccactgt ctggacctat
      121 atcgataatc caatcagaag cacgtataac ctctgtattg tgttcaataa ttaaaagtga
      181 atgctgttca tttacaagtt ttctaaaagt aatcaacaaa ttttctatat ctttaaaatg
      241 aagtccagta gtaggctcat ccaatatgta cagcattcgt tttttagttt ttgtccctaa
      301 ttcttttgct aattttatcc tttgagactc tcctccgctt aatcctaatg tattttgtcc
      361 aagctttaga tatccaagtc ccacttcatc taataaggag agttttctat atatatcaga
      421 gcaatcacta aaaaatgttt ttgcttctgc tacttccatg tcaagtatat ctgcaattgt
      481 ttttcccttg tattgtatag acagtatttc tttttgatat ctcttacctt gacattcatt
      541 acaagttacc cattggtctg ccatatattg tagaataatt tttgttttac ctaatccttg
      601 acagtttggg cattgcccct ttttgctatt aaagctaaaa tagctttcat ccaataattt
      661 ttgttttgct tgtggagttt cagtaaatag agaacgaatt tcatcaaaaa tattgatata
      721 tgtagcaggt gtagaacggc tagattttcc aataggtgtt tggtccatta agataaaatc
      781 gtcaatagca tcaaatccag taagtgcagt ataattttta tttgggatag atttttgttt
      841 caatttttct tctagagcag gaagtaaact gtgaaataca agagaactct taccagaacc
      901 actaacacct gtaatagagc acatacagtt taagggaatt tctacatcaa tattttttaa
      961 gttatttgca tggcatccct ttaagcttaa ccatttactt ggtataattt tattttgtga
     1021 acttatctta ttgtatgtac tcaaatattt gcctgttata gaatttggat tttttataat
     1081 ttcttgggtt gtaccttctg caatgatgaa gccccctttt gtaccagcat ttggtcctac
     1141 atcaataata taatcagcag acagtattgt atccttatca tgttccacta ctataacagt
     1201 attacccttg tctcttaatt cttctatcat tttgatgatt agattatggt ctcttggatg
     1261 taaaccaata gatggttcgt ctaaaatata ggttaatcct actaattcac ttcctagctg
     1321 acttgataat cgtactctct gcagttctcc tccagataaa gttggagctg ttctatctgt
     1381 tgatatatag ctaagaccta cctttaatag ataactaact cgaattttta attctgttaa
     1441 gatatcattt accaaagata gctcattttt ttgaagttga tttggtaatt cacacaacca
     1501 attccataac tgttctatcg tcatttttgt tagttcaggg aagcgataac ctttgattgt
     1561 agtgaatcta gcctcaatgc acagcaattc gccaccacat gtattgcaag gaattttgtt
     1621 catatattga tgcaaggtat tattttcaga actagattct ctaaaaagtc tttgaatgtg
     1681 gttgattgct cctgaagcag gccttctaat ctttgattct cgtccctttg attcaaagct
     1741 aaattcatat atcttatcgt ctgtaccata taagattgca tctataaaat ctcttggcaa
     1801 ttcattccaa ggtatatcta ggtctatatt tttatctttt gctattgcat ataattcacc
     1861 taccatccaa ttaccatttg gctttttccc cctcaatttt ccaaaatagt aagtagctcc
     1921 atctaatatt gaaatagttg ggtcttttac aattaattga ggattaactt gataggtaaa
     1981 acccaatcca ttacaatcgt gacaagctcc agtatgggag ttagcattga aggttgaagt
     2041 atttatctta gaaaaaatag tttggcaatg tggacaataa ttttttgtat tattcttttt
     2101 ttctaatgga gttttgcaat atggacagta actttttccg attgatacaa ataacattct
     2161 taagtaatcc caaatgcttg taagagtacc aacagtagaa cgaggatttt ggcttccttt
     2221 tttctgttcg atagctatag taggggttaa tccctgaagt aaatcaaatt ccggtttttg
     2281 aagctgttct gaaagttttg ctccattacc tagattttca aaataacgtc tttttccttc
     2341 ttcataaatc gtatcgaaag ctaaagaaga ttttccacta ccacttacac ctgtaataac
     2401 agttatttga ttttttggaa ttgtaacctc aacatccttt aaattgtttt gccttcctcc
     2461 ttttagatag atagagcttt tattatgaca aagtgaattt ttaattgatt tatttattgc
     2521 ttttgcaaat ttgaatttct tttctagttc aagaggattg cctaattctt ttatttccga
     2581 aatacctaat atttttaact gagaaaagat tgatttcaaa taatcttcag gcaatttttc
     2641 tattaacaaa ttaaggtctt ctatttttat atttccgctt gataatatct ctgccatttc
     2701 atctaataaa tttttttctg ctcttgttaa ttgattgtat tttttggaac ttaattctgt
     2761 ccataaatcc catggaagca attctaaacc aatcatatta ttaaagtcat gtagaagatt
     2821 accctttaca gaatgccaac ctttccaatt tttattatat ccaaagtcat ctggatgagc
     2881 atgtccttgt cgacatctta tccaggcttc tgaaccagtt aaaaaaccat ctgtttttcc
     2941 gacatcatga gtgtcaaaat taatttgaaa ataatttttt tgtatgtcat ctatttgtgc
     3001 atctattctt atccatctct ttgttagagt atcatagtat tctacaagcc agtggtcttc
     3061 ataagtaagc tcattttcaa aataattagc aaaacctgct ctcattctag cctcaaaacc
     3121 tcgatatctt aggagcgata caagaagtaa agaataatct ctgcacattc caacaagcct
     3181 ttgttttggg tttcttggaa tagttagtgg gttagaattt atttcaaata ttctttttaa
     3241 catttgagga attgtacgaa gcaattcctc atcagattgt tgcttattaa aggatatacc
     3301 atataatttt ccttggtctc catgaattac taggctttga accactttta ataactcaat
     3361 tggagtcttt ggtaaatctt gatatttatt ttcaaatttt ttaggatttg tgatgatgct
     3421 ttgattttta tagttattta ttttcattta tatttcacct ctctaaatgg atgatatacc
     3481 ctgacatggt gtcatatgca aggacaattt agattttttg gccaaaattt aaaaataaat
     3541 aaaaaatgat acagtgataa attgtaatac aaatatagtt tcaaattgca tataatataa
     3601 ttatttaata aaaccattat ctatggaatg ttaatgaaat gttaagatgg cattcttgaa
     3661 tattaagaaa tagaatacta taatatagtg tgaaggattc ttagtaaaaa aatttgtaag
     3721 tgttaatata cattaaaaaa taggtttata atttggagga aaaaaatgaa attatcaagg
     3781 tataagaaaa atagtttagt tctgtctata attttatgtt cacttattat ttcaatcctt
     3841 gtaattactg tttttaatac cgtaaaacta cacaatatat ctaataaaca atctaaaaac
     3901 tatttaaacg atgtatctac acagattgta atgaatgttg attctaaaat caaatttatt
     3961 ttatccgact taagaataat ggcagatttt attaagcaat atgaaggtga tagtcgatat
     4021 gagtatttat ctaaaagaaa gactagttat aagtattatg atgttggaat aatagattta
     4081 gaaggtaaag ctgagttttt aagtggaaaa aagtttgact taaagaatac tgcttcctat
     4141 aaaaaaggaa ttgaaggtaa agaatactca gaagtaatag agtcaatggg ttttgttctt
     4201 tatagtgtac ctatatttaa cgataatcat gaagtgtcta gtattttaat aggtgtatcc
     4261 aataaagaaa gcatggatga gattcttaat atagatagct ttaatggaga aggaactatt
     4321 gaaattatta atagtcaggg aaaaactctt ttcataggaa aaaataatga gttaataaaa
     4381 gaccttagct ataagtataa ggattctgca gatgaacctt gggcacaaaa gatgcttaat
     4441 gattttaaaa atgataaatc aggaaatatt acaataacct catcaaaagg tgtacaatgc
     4501 ttattaactt atcatccaat tacaagaggt ttaaatgatt ggcattttct tctcatagta
     4561 cctgaagatg ttgtacttgg tgaacttaat aagttaaatt cttttacaat aataatgaca
     4621 tttataacta catgtattat tggaattata acattaattt tatatattat aagaagaaaa
     4681 tatgtaaatc aaatagaaaa tattgcatat ttagatagta taacaaatgg tataaattca
     4741 actaagttta gtatgctagt caaaccttta atttcagaat cacctgatag tacttatatg
     4801 atgatagcaa tgaatataaa agattttaag ctaataaatg attgtttcgg tagtgaaaaa
     4861 ggaaatatga cactaaaaca tctttacaat attttgtata aaaacattga tgaaaatgaa
     4921 gaatttgtgt gccgtcatga tgcagacctt ttctatttgc ttattaagaa tagaccagtg
     4981 ttagaagccc tggagttatt atataaaata gaagctgaca caaattattt taataaaaat
     5041 agggaaaatc catacttttt aagactatca gtaggtattt acacaattga aaatcataat
     5101 gaagatttaa ttacaataca agaccatgca aatacagcaa gaaaaagctt taataaaact
     5161 catcaaagtg atttttcttt ttatagtgat atagaaagaa aacgattaat atctgaaaaa
     5221 gaaatttcaa atttaatgga aaaagccctt gaaaataaag aattttttat gtgcttacaa
     5281 cctaaaattg atataaaaag tggaaaaatt agtggagcag agtctcttgt gcgttggaaa
     5341 aattcagaaa agggcataat ttatccaagt aattttattc ctcttttcga aaaaagtgga
     5401 tttatatgta agttagattt atatatacta gaagaaacat gcaaattgat ttctaagtgg
     5461 ataaaagaag gtaaagaagt gttgagaatt tctgttaatg tatcacgtca acatttgaat
     5521 gataaattgt ttttagaaaa atataagcat atatgcaata aatacaatgt accaacatgt
     5581 ctgatagatt tagaacttac cgaatctatt ttcttagaaa atccagaagc tatagatata
     5641 atagaggata ttcattctaa tggatttaaa tgttctatag acgattttgg atttgggtac
     5701 tcatcattag ggatattaaa ggattttaaa gttgatataa ttaagttaga ccgttcattc
     5761 tttgttagca aaaataatat agaccgtggt aaggtagtta ttaagtctat tattgaactt
     5821 agcaaaaggc taggtatgaa agtaactgct gaaggaattg aagaattaga acaagttgag
     5881 ttcctaaaaa atgtaggatg tgattatatt caaggttatg ttttttctaa accattatta
     5941 atttctgatt ttgaaaattt tgcatatgaa aataataaaa ttaaaaatat ccattattaa
     6001 atgtacaaga taaaaaattt aggcaagtta tataattaaa aatataactt gtctttttta
     6061 tttatatatt taataaaaat atatttgcag attttgtaaa aataattttc aaatgttgta
     6121 agttgtggct aaagctttat tgtattattc tgaatataga aagatagtta ctgtcttaga
     6181 taaaaactga aacatacata ataatataaa atttgctata ttaataactc taattatttt
     6241 ggaaggaggg aaaatatgat aagtgggaga atgttactca tcatgaattt tctaaaagga
     6301 aaaaataaga caagttacaa ggatattagc aaacaactta gtatagaaga aagaaaagtt
     6361 agatatgata taaataattt aaatattgtt ttgcgttcat taaacaagtc agtaatacaa
     6421 aagatgaaca aaggtgtgtt aattattcct gatgacttcc aagtagtatg tataaataat
     6481 aacgaatatg tattttcctt aactgagcga gtatctatta tgaaaattat atcaatgttt
     6541 aaaatagata atctaaactt agaaaaattg agtaaagaat ttcaggtttc tagaagttca
     6601 attaaaaatg accttaatgt tttgtcaagt gaattagaaa gaaaccaaat aactattact
     6661 tatgataaat cttttaaaat aagtggagaa gaagacctta tatataagca aagattgaac
     6721 atattaaaat cgtattctta tctttttggt aaggaaaaat ctgatttaag tgtatttgag
     6781 aagtatttag tgaatgtaat tgaaaaaata ttcaaaggga tttatttgat tgatatatat
     6841 aattggtcta tgaatttatt attgcaaatg ggatggactt taaatgatga atcttttaca
     6901 tggtatgtgt caaacatttt cctttttaca tggtatatac attcagatga aaaacatcct
     6961 ttagaatctg cttcattaag tgagccatgt tttgaaaata agtttgacca agaatttgaa
     7021 acaattattc aaaaaaagtt gactaaggaa caaatttttc tgttaaagag ttttgtattt
     7081 tttacaaata aatatgcaag tttaaacgag gatatggatt tgattagtac agaaattata
     7141 gttcaagacc taatttacaa tatgtcagaa atattagaga ttagctttaa agaagatatg
     7201 attctttata aaggattact caatcatatt gccccattga tagagagaat caagcagaat
     7261 atacagattt atgaggaatt acttcatgta ataccagata aatatcaata tgtacttgaa
     7321 gctactaagg tttctattca aaaaaacaga gttttaagtc aaataaagaa tgaaaatgaa
     7381 ataatacttt tggcaataca tttcttagga agtatacata gaaatgagag gaataactac
     7441 aaaaatatat tgttagtttg tggatttgga tatggtgtaa ttgctatgtt aaaggatagt
     7501 ttagtaaatg attaccagat aaatatagta gagagtattc catcatataa gttacaagat
     7561 tataaagaat gggaaagtat tgacttcatt ataacaacat caaagatagt aatagacttg
     7621 cctaaaacaa ttattgagat aaatccattt ttacaagaag aggattattt gaagtttgaa
     7681 gaaaaaggga taaaaagaaa gaacgtctta actaattata tgtcaataaa aaaaaggttg
     7741 gactttttag aagaagatgc tcaaaaaaga gtattaagta ttatatgtca agaacttgga
     7801 tacagtgatg agagaatatt atttagacaa cttaatttaa gtgatttaat agggattgat
     7861 accataagga taattgatga agatatgaaa tgggaagatg ctgtaaaaat tagttcaaac
     7921 atacttgcta attgtgattt tgtatcagca caatatgctg agaatattat agatatttta
     7981 aaaaatgtgg gtttttatgc tgtaaaagat aacgaatttg ctttactaca tggcaatgat
     8041 agttcattag tcaaagtaag ttcgataagt ttattaatat gtaaagaagc agttagtttt
     8101 ggagataaaa aggttaaaat catattttgt ctagcaagca gagataaaaa agagcaaatt
     8161 cctgcaataa ttaatctaac taaaatggtt tataagacta attttattgc tatgttagaa
     8221 agtgcaaaga caaaagaaga agcagaagtt ttaattcata aatatgagaa ggaggtcact
     8281 gtatgaataa attagttaat gtaaaatgtt catttaaaat acatgctaat tcaaaagaag
     8341 aagctattgt atcgttagtt gatgtagtat caaaagaagg atatctaata gataaaaatc
     8401 aatttttgga agatgtttta aagagagaag aaacattgtc aacttatatt ggacatggta
     8461 taggtcttcc tcatagtcaa agtgtaggtg ttaaaaactc ttgtattaca ataggaaagc
     8521 ttgatattcc tattgaatgg acagaagaag gagaaaaagt ggatttaata tttttaatat
     8581 cagtaacaaa agacaatgaa aataatcttc atttaaagat tttatcaaaa ttagcacgtt
     8641 tattgatgca tgaaagtttt agaaatcaaa ttagatgttc agatgaacaa actgtttata
     8701 atcttataaa agaaaaaata gaggaggaag attaaaatga gaattttaaa gtttttgcaa
     8761 aaacatctta tgacagcaac atcttatatg attccatttg tggttgcagg aggaatatta
     8821 tttgctttat cagtaactct aagtggacaa gctgctgttc ctgaaacagg ctggttggct
     8881 aaactgaatc aaattggagc tgctggcttg gctttattca ttcctatatt aggtggatat
     8941 atagcattta gtatggcaga taaaccagga ttagctccag gtatgatagg tgcatattta
     9001 gcaaaagaag taaatgcagg ttttataggt ggtatcatag caggatttat agcaggattt
     9061 gttgttttac aattaaagaa aattaaatta gctcctacaa tgagaacttt aggttctata
     9121 tttatttatc ctttgattgg tacattgatt acaggtggta taattgtatt tttaattgga
     9181 gaacctatag cttcttttat gacatggatg acaaactggt taaatggaat gtctggtgtt
     9241 tctaaaattc ctttaggtgg aatcttagga ggaatgatag cctcagatat gggtggtcct
     9301 ataaataagg ttgctgctac ttttgcccaa actcaagttg acacacttcc atatttaatg
     9361 ggtggagttg gcgttgcaat ttgtatacct ccaattggtt taggattagc tacattattg
     9421 tttccaaaaa aatttagcaa ggaagaaaga gattcaggta aagcttcgct attaatggga
     9481 tgtgttggaa ttacagaagg agctattcca tttgcaacat cagatccagt tagagtaata
     9541 ccttgtatta tggctgggtc gattatagga aatattatgg cttttttatt aggatgttta
     9601 aatcatgcac cctggggagg tctgatagta ttacctgtag ttgacaatag gttggggtat
     9661 attgcatcag tattaactgg agcagtagtt gttgcagtat taatgaaatt agtaaaaaaa
     9721 gacgtgaaag aagatgaaga aattgaagag gatttagatg aatcaattga attaatattt
     9781 gaagaattat aaatataaat ggaaggaatt tgtataaaaa agtaaatata aaactataaa
     9841 cttaaaggag ataagaatat gaaaattgta gcagtaacat cttgtcctag tggagtagca
     9901 catacttaca tgtcggctga atcacttgaa ttatcggcca aaaaatttgg tatagaaata
     9961 aaggttgaaa ctcaaggaag ctctggaata gataatgaat tgtcacttaa agatattgat
    10021 gaagcaactt gtgtaatatt gactaatgat gttgagatta gaaatatgga acgttttaag
    10081 ggaaagaagg tagtaagaat gagtgtatct gagataatta agaaatctga tgcattaatt
    10141 aagaaaataa aagatacttt tcaataaaaa ttgatttatt ttacatataa ctcaaagatg
    10201 tttttaaaga aatttttatt aaaagcatct ttgaaaatat cttaaaaata gaataaaagg
    10261 agatatagtc atgaaaaaat taccaataaa gactgtcgta caaagattac tagacttaca
    10321 agaagagggg aaaagtgcaa ctcttttagg aattggtcct atgtcaccaa acttgttaca
    10381 agcaagtttt gaactagcaa aagatgatga ttttccttta atgtttatcg caagtagaaa
    10441 tcaagtagat gcagatgaac taggtggcgg ttatgtaaat ggttggaatc aggaaacttt
    10501 tacaaaagat attaagaaga ttgccgacaa agttggattt gatggtttat attatttatg
    10561 tagagaccat ggtgggccat ggcaaagaga taaagagaga aatgaccatt tgtctgttga
    10621 tgaagcaatg gagttaggaa aaaaatctta tttagcggac attgaagcag gttttgacct
    10681 attgatgatt gaccctacaa aagacccatt tgaaattgga aaagtaattc cactagatgt
    10741 agtactagaa agaactgtag aattaattga gtattgtgaa aacgagagaa aaagacttaa
    10801 tctaccagac ataggttatg aggttggtac agaggaaacc aatggaggtc ttacttcaac
    10861 tgagacgtat gaaacattta taactagact aaaggttgaa cttgataata ggggtttgcc
    10921 aatgccaaca tttatagttg gtcaaactgg tactttaact agaaagacag aacaagtagg
    10981 aacttttaat tttagaaatg cttatgattt agctcaaatg gctaagaaat atggagttgg
    11041 tttaaaagag cataatggcg attacttaga tgatgtaact ttattagaac atataccatc
    11101 tcaaattatt gctacaaatg tagctcctca atatggtact gaagaaacta gggcatattt
    11161 aaaattagca gaagttgagt gtaaattaga aaaagagggc ctagttgata aaacttctaa
    11221 tatcagacat gttcttcttg ttaatgcaat tgaatgtgga cgttggagaa aatgggtagt
    11281 aggagaacaa aaaaatctaa ctactgaaga gatatttaag aatgaagttt tatctaatga
    11341 aatactagat attgcaggac attatacatt taataatgat gatgttaaaa aagaaataga
    11401 agtgctttat gataatttaa gtaagaataa tattgatggt caaagatttg ttgtagacca
    11461 tattaaacgt cctttgcgtg attatgcaga atgctataac ttaaaaggtg ttactacgag
    11521 gattttaaat aaataatagg acttatagtg gtgagtattg ttatactagg tatttatagt
    11581 tataattata cttaccacta ttaaatttag gaggactaat attttgatta atttaatttt
    11641 tgatgtagat gatactttat ataaccaatt aacgcctttt tacactgcat ataataaagt
    11701 gttttcttca ataaaggata tttctattga agatttatat atgagtagta gaaagtatag
    11761 tgatgaggta tttcatatga ctgaaaatgg agaaatgcca atcaaagaaa tgcatattta
    11821 taggataatg aaagcttttg aggagttggg aaattctatt acagaaaaag atgcacaaag
    11881 ttttcaagat gagtatatat atcagcaatc acaaattaca ctaataccag aagttgagag
    11941 gattttaaat ttttctaaag aaagaaatat taatctagga attataacta atggcccatc
    12001 aaaccatcaa agaatgaagt taaagcagtt aaatattgaa aattgggttg ataaaagtaa
    12061 tatttttatt tctagtgagg ttggattttc taagccagat actaatattt ttagagtagc
    12121 tgagaatgtt atgaatttag atagagaaaa tacatattat gtgggtgatt cttatagaaa
    12181 tgatgtatta ggtgctaaaa aagctggttg gaagagtata tggttgaacc atagaggaca
    12241 tgaggtagaa gagttgtttt ataaacctga ttttgtaatt ttggaacata aggatttgat
    12301 atctttattt ataaaaatat gtagttataa aaatatgtag taaaaaaatg ttaataatag
    12361 aattgtataa aataattaat aataactata taaaattata tgtttagatt gtgagcaatc
    12421 tattttatgt taaaaataga ttatttagaa gaaggggaaa tcttaaaaaa atttgaatat
    12481 ccccttttta ttatttaatt aaaatgaaat agttagaaat atattttatt tagtttagtc
    12541 aataacattt tgggattctt tataactaca tgttaactgt tgaataggct tatcatatgg
    12601 tttaacccat cctttattaa tacttaattc tgtaagtgct gtgtgacctt ctaccatttg
    12661 agttagtgaa gctgaatata tagctcttaa ttcaggtgta ctacttgtta atgcagaatt
    12721 aagatacata ttggcagatg ctttagcaga tgaaagcata cttaacatta ttactttatc
    12781 gtctatataa gtattgttct taattagatt ttctatcata tttctcatgt tatctatacc
    12841 tcctcagaaa ttagtatttc attttcaact ataaattgtt gtatagcttt aattcttcct
    12901 tctgttgcta atatactaga ttctgattgt ctttttaggt cttcatctga tattaacata
    12961 tttattgctc tttgcataag taatccgtca gactccattt ttataactgc tgctaaagat
    13021 attttttcag tttctgaaag tgttctcatt taatcatctc ctatattgta atttatgctt
    13081 taatagtatg tctttctttt caaggaaata accattacac ttttttacag tattttatat
    13141 tctctttata tcaaagttta gtcaacttaa tatattaata taattttgta ggatatttat
    13201 cattaaatat ctataaaata aattatttga atattcataa atcaaaattt atttgtatgt
    13261 acttcttcaa gagtcaatat gaattttttc aaaaaattaa ttttgatgat aatcattaag
    13321 ggctatattt ttcctaaagt tgtgattatc attattaatt tactaaaata ctttaatgaa
    13381 aaatagctaa ctttaattgt aggatattct taatatattt gaaataaata aaaatattga
    13441 aggatataat attcaattgg tatataaata ataaatgata gttaagattt accttagagg
    13501 ctaattaggt gttcagtcaa aattaatgcc tttagaatac aaatgtattc taaaggcatt
    13561 tttactatgt attttcattt atattactaa acattatcta tttttatata catttctatg
    13621 tattttattt tcatcaaatc ggtctataaa ttcatttttt tcttcactat atccaataga
    13681 tataagtgca taaggaacta attttactgg taagtctaaa atttgtttaa ctttatttac
    13741 tactttattt ttaggaaata ctcctgccca acaagcagct agaccttgat tagtaacttc
    13801 taataataaa ttttgtgtgc aagcacctaa atcttgtatc catttaccag gttttaagaa
    13861 tttatctcta tttccaagca ctactataca taatggtgca aattctatat gctttgctct
    13921 atgctgtgac tttgagagct ctttcagcag ttccttatca tctactataa taaattccca
    13981 aggttgagca ttttttgatg aaggtgcttg catagctgat tttagtattt cagttatttt
    14041 atcttcttct atatttttat ctatatatgt tcttattgaa cgtctatttt ttataacttc
    14101 taacatacag attctccttt aaataaaatt atttctattt atataatatc attactttaa
    14161 gttttagcta ttacttatat aaagaataaa ctttgtgttg gttttatcta agtaaaagta
    14221 acattcagaa ctaaaaaaca agactataaa tccaagatat taattaaaaa agaagtaata
    14281 aaaaataata tacaagattt aaaaaagagt attgagctta aaatttaatt agaaaaccaa
    14341 ttttgagata atctgttttt tattttaaca aaaaaacata aataaagttc gatacgtagc
    14401 gaatgaaaaa aatacaagaa atattttttt taaaaaattt caaaaaagag ttgataatat
    14461 atgtatatta atataaaatt taaggtgtat aattcgtata ataatacgaa tgatttgtag
    14521 tgtaaaataa cttataaacg gagaggggaa gtctaatgaa agtagcagta gttatgggtt
    14581 caaaatcaga ttatccaaaa ttagaagaag ggataaaact gcttgaaaaa tatggaatag
    14641 aggttgtagc aagagcatta tcagctcata gaacaccaga acaactttca atattcctaa
    14701 aagaaataga agatgatact gatgtaataa tagcagcagc aggaaaagca gcacacttac
    14761 caggtgtaat agcttcacaa acattaattc ctgtaatagg attacctatt aaatcatcaa
    14821 ctatggatgg actagactca cttttatcaa tagttcaaat gccaaaagga atcccagttg
    14881 caacagttac aatagactta ggtttaaacg cagctttatt ggcattacaa ataatgactt
    14941 taaaataccc aaaattaaaa gaggatttga aatcatatag agaagagatg gcacaaaagg
    15001 tgttagaaga cgacaagaat ttaagggggt agcagaaatg ttattatatg aaggaaaagc
    15061 aaaacaagta tattctacag ataatgaaaa tgaatatgtt gtttattata aagatgatgc
    15121 aacagcattt aatggagaaa aaaaagcaga aatatcttca aaaggtatac taaataataa
    15181 gatatcaaca ataatctttg aaatgttaaa agaaaataat ataaatactc actttataaa
    15241 aagtttatca gatagagaaa tgttagttaa aaaagtagag attttaccat tagaagtaat
    15301 agtaagaaat attgctgctg gttctatctg taaaagagtt ggtcttgaag aaggtgtagt
    15361 tttcgatgag ccaatttttg aaataagtta caaaaatgat gcttatggtg acccaatgct
    15421 aaatgatgat tatgcagtgg caatgaagtt agcaactaga gaagagttaa aattcttaag
    15481 agaagaaact ttaaaaataa acgagttact aaaagcattt ttcttaaaat taaacctaaa
    15541 gttagttgac tttaaaatag aatttggtaa ggattctgaa ggaaatataa tattggcaga
    15601 tgaagtatca ccagacactt gtagactttg ggatgtaaat acaaatgaaa agttagataa
    15661 ggacagattt agaaaagacc ttggagacct tgtagaaggt tatacggaag tactttcaag
    15721 aatgaataat aaataggagg caaatgtatg tgtggagttt tagggattta ctctaataaa
    15781 gatgtaacta aagaactata ttattcttta tattctatgc aacacagggg gcaagaaagc
    15841 tgtggtttag ctcttttaga tgatggagaa attaagtaca aaaaagatat gggattagtt
    15901 ggagatgttt ttaaggaaaa tgaattatct aagttaaaag gaaatatagg gattggacat
    15961 gttagatatt caacagctgg aggaagtcat gtttctaact gtcaaccttt agttggaagt
    16021 tgtagaaaaa gacaattagc catagctcat aatggaaatc tagttaatgc aaattattta
    16081 aaagatatgc tagaagaaga tggatatatg ttccaaacta attcagatac agaagtgatt
    16141 ttatatatac ttgctagata ttacaaaggg gatatagtag aaagtttaaa agtgacaatg
    16201 gattatataa aaggtgcata tgctcttgta ataatgagtc aagaagaatt ggttgcagtt
    16261 agagacccac atggattcag acctcttgta cttggtaaaa agggtgatga atatatattt
    16321 gcatcagaaa attgtgcaat agatatactt ggcggagaag ttataagaga tgtagagcca
    16381 ggagagataa tagtagtcaa agatggagaa ctaaaatcat atttttattc agaaaattat
    16441 aaaccagtta agaaaagttg tatatttgaa cacatatact ttgcaagaaa tgatgcaact
    16501 atagacaatg taaatgcata tgaatttaga attaaatgtg gtgagagatt agctcagaat
    16561 gaaactgtaa aggcagatat ggtagttcca gttccagatt caggttggcc aggagctatt
    16621 ggttatgcaa atgctagtgg attaaaaata agtgaaggct tagttaaaaa tagatatgta
    16681 ggaagaacat ttataaaacc tacacaagaa gaaagagaaa tagcagtaaa aataaaatta
    16741 aatcctcttt caacaataat aaaaggaaaa tctataattt tagtagacga ctcaattgta
    16801 agaggaacta catctaaaca attagtaaaa tctttaagag aagcaggagc aaaagaaatt
    16861 catcttagaa taacttctcc tccagtagct tactcttgtt actatggaat agatactcca
    16921 aatcgctcta aattaattgc atcaagtaac aacgtagaag aaatgagaga atatattgga
    16981 tgtgatagtt taaaattcct agatatagaa ggaatgttag atgcaacaga acataagtct
    17041 actttctgta aagcttgttt tgatggagaa tacccagtta agaaaattga taaggaggaa
    17101 ctactttcat gttaacatat aaagaatcag gtgtagatat agatgaagga aatagagcag
    17161 tagaccttat aaaaggtaaa ataaaaggaa cttatgatgg aaatgtagtt ggagatttag
    17221 gtaacttcag tggattatac agtttaaaag attttgtagg tatgaaagaa ccagttttgc
    17281 ttgcatctac agatggtgtt gggactaagt taaaaatagc tcagatgatg gacaagcatg
    17341 atactgttgg aatagattta gttgcaatgt gcgtgaatga tttaatttgt caaggagcaa
    17401 aaccattatt tttccttgac tatatagctc ttgggaaatt agtgcctgag catatcgaaa
    17461 aaatagttgg tggaattgca gatggttgta aaatgtctgg ttgtgcacta attggtggag
    17521 aaactgctga aatgcctgga atgtatggag aagatgacta tgatttagca ggattttctg
    17581 taggaatagc agacaaagaa aagattgttt ctggaaacaa tgttaaatca ggagatgtat
    17641 tggttggtat atcttcaagt ggagtacaca gcaatggatt ttcttttata agaaagatat
    17701 ttttagaaac ttacaattat aaaatggaac aatatgttga agaactagga atgacagttg
    17761 gagaagcact tttaacacca actaaaatat atgttaaatt agctttagat gtattagcta
    17821 aacatgacat aaaagctata gctcacataa ctggtggagg gcttatagaa aatataacaa
    17881 gagttatacc taaagggtta ggactagata tcaataaaaa atcttgggag aagcctccta
    17941 tatttaaaat gatagaaggt tttaatgctg ttgatgagag agaacttcat aagagtttta
    18001 acatgggtat tgggctagtt ttaatagtag ataaagaaaa tgctgatgat gttgtaaact
    18061 ttataaataa tagagaaaat gacaatgctg cttatgtaga taaaaaatac agtgaattat
    18121 tagaagataa agcgtatata attggtgaag tagtagatag tcatgaaggt gtagaattat
    18181 gctaaatata ggagttttaa tatctggtgg aggaacaaat ttacaagcag ttatagatgg
    18241 cactgagtct ggagaaataa aaggtcaggt taaagtagtt atctcaagta agcaaggtgc
    18301 ttatgggcta gagagagcta aaaatcataa cataaaagca atttgtgaaa ctgatgaaga
    18361 caagataata gagattctta aggaaaataa aatagattta gtagttttgg ctggttattt
    18421 aaaaataatc agcccaaagc tggttaatga gtttagaaac aaaatgataa acatacatcc
    18481 atcattaatt ccttcatttt gtggagctgg gttctatgga gaaaaagttc atcaaggtgt
    18541 tatagattac ggagctaaag taactggtgc tacagttcat tttgttgatg aaggtgctga
    18601 tactggacca atcataatgc aagatgtagt taaggtaaat caagatgatg atgcaaagac
    18661 acttgcaaaa agagtgttag aagttgaaca taggatttta aaagaaagta taagtctttt
    18721 ttgtgaaaat aaattaaaac ttcaaggtag gagagtgttt ataaatgagt aagagagctt
    18781 taataagtgt aactgacaag actggtgtag tggaatttgc taaggagtta aataagttag
    18841 attatgaaat tatatctaca ggaaatacat ttaaaacatt aaaagaaaat ggtgttaatg
    18901 taatgcaagt tgaagatgtt acaaattttc cagaaatatt agatgggaga gtaaaaacat
    18961 taaatccata tatacatggt ggtatacttt ataaaagaga taaagaatct catgtagaaa
    19021 ctgtgaatga gcataaaata cattcaatag atttagtagc tgtaaatcta tatgattttg
    19081 aaggaacttt aaaagctgga aaaagtcatg atgaaataat tgaaaatata gatattggtg
    19141 gaccatctat gatacgttct gctgccaaaa actataaaga tgtaatagta gtagtagata
    19201 taaaagatta tgattctata atagaaaaat taaaaacaga tacaatgaca ttagaagaca
    19261 gaaaaaaatt atcatataaa gctttctcaa ctacaggaag atatgatgcg ttaatatcta
    19321 gttattttgc tggagaagtt ggagatacat atcctgacat attaaattta actttccaaa
    19381 aagaacaaac tttaagatat ggagaaaatc cacatcaaaa tggtttctta tattcacaat
    19441 caaatgcaaa aaatccaatc ttaaattatg agcaattagg cggtaaagaa ctttctttta
    19501 acaatttaaa tgatttacat ggatgtttag aagtaatgag agaatttaaa gatagtgaag
    19561 aagtagtttc agtagctata aaacatgcta attcatgtgg ggtaggtcta ggaaaagatg
    19621 cttttgaagc atatacaaaa tgttatgaag cggataaggt ttcaatattt ggaggaatag
    19681 taggaataac ttctactata gataaagcta cagcagagaa attaaatgaa atattcttag
    19741 agatagtagt agcatatgat tttgagccag aagctttaga ggtacttaaa caaaagaaaa
    19801 atttaagaat attaaaatta gctaagatag aagatagttt acaaccatat gaaatgaaat
    19861 atttagatgg aaaattatta atacaagata gaaataatat attagcagaa aaaagtgaaa
    19921 atgtaacaaa agcaaaacca acagatgcac aacttaagga catggaattt ggtatgagag
    19981 tagtaaaaaa tatgaaatca aatgcaattg cgatagtaaa aaatggtcaa actttagctt
    20041 taggttgtgg acaaacatca agaatttggg cattaaaaaa tgctcttgaa aataacaaag
    20101 ataaagattt tacaggagct gttttagcgt cagatgcatt tttcccattt gatgattgtg
    20161 taacattagc tcatgaatat ggaatatcag cagtagttca accaggtggc tctataaagg
    20221 ataaagattc aatagaagct tgtgataagt atgatatggt tatggtattt acaggaataa
    20281 gacacttcaa acactaagga ggatattatg aagatattgg ttgtaggcgg tggcggaaga
    20341 gagcatgcta tatgctggaa actaagcaaa gaaaaaaatg tagaaaaagt ttattgcgcg
    20401 ccaggtaatg caggaattac aaatgtagca gaatgtgtaa atataggtga tactaatata
    20461 gaagaacttt taaaatttgc caaagaaaat gaaataggtc tgacaatagt aggaccagaa
    20521 gtaccactag ttatgggaat agtagatgag tttgaaaaag aaggattaag agtatttgga
    20581 cctaataaaa aatgtgctca acttgaagga agtaaagctt tttcaaaaga atttatgata
    20641 aaacataaca tacctactgc aaaatataaa gaatatacaa acttagaaga agctatatca
    20701 gaaatagata gttttggata tccagtagta ataaaagcgg acggattagc tgctggaaaa
    20761 ggtgttgtaa tacctgaaaa tagagaagat gctatagcaa ctttaaaaga aatgatgtca
    20821 gataagaagt ttggagctgc tggagataaa atagtaattg aagagttttt aaaaggaata
    20881 gaaacttcaa ttctagcatt tgtagacaat gatacaatag ttcctatggc aagtgcaaaa
    20941 gaccataaaa aagtaaacaa ctatgagcaa ggtccaaaca ctgggggaat gggaactttt
    21001 tcacctagtg aaatatatac agaagaatta gcaaataaag taaaagagac agtattagaa
    21061 aaaactttag aaggcttcaa aaaagatgga cttaatttta aaggtatttt atttgttggt
    21121 cttatgataa ctgaagatgg agaaaaagta cttgaatata atgtaagatt tggagaccct
    21181 gagacacaat ctgttttatt tagattagaa acagatttac atgagataat ggaaactata
    21241 ctagataata agttaaaaga tatagaaata aactatagtg atgatgaagc tgtatgtgtt
    21301 atgcttacat caggagggta tccagacagt tatgaaaaag ggaaaattat aactggtctt
    21361 gaaaacttgg atgatgatat agtagtattc catagtggca ctaagatgct tgatggtaat
    21421 ttagttacca atggaggaag agtaattggt ataactgcaa agtcaactac agttaaggat
    21481 gcagcagaaa aagtttatga aaacataaag aagataaact ttgaaggaat gcactataga
    21541 acagacatag gaagataatt tttctaaaat ggaggataag gtatgttgaa tactgaaaat
    21601 aaagactcaa tggttagaag agtattagtt gaaaaaagag aaggttttga ccttgaggct
    21661 aaagctttaa aaaaagactt agtagaaagt ttacacatag ataatataga aaatttaaga
    21721 atattaaata gatatgatgt agaaggaata tcagaagaag tttatgaaaa tgcagcaaag
    21781 acaatattct ctgaaccaaa tttagatgta gtatattatg aagaaatacc aaaattaaat
    21841 gatgaaagag tatttgcaat agaatttctt cctggtcaat acgaccaaag aggtgattgg
    21901 gcagctcaat gtgtacaaat tgtaaatcaa gggataagac ctgctattaa tacagcaaaa
    21961 gtttatatat tatcaggcaa aataactgat gaagagtttt ctaaaataaa agattactgt
    22021 ataaatccag tagatagtag agaagcatct ttagaaaaac cagaaacatt aaaaatggaa
    22081 actgaaatac caacaacagt tgaagtatta gatggattta tagatttaga tgaaaatgga
    22141 ttaagaacat ttgtaagtga aaaaggatta gcaatgactt taggagactt acaacacgtt
    22201 caaaaatact ttaaagatac agaaaagaga aatccaacta taacagaaat aaaagtatta
    22261 gatacttatt ggtctgatca ttgtagacat actactttta tgactgaaat agaaaatgta
    22321 aagatagaag atggtaagtt taatgatata gtaaaagaag cgtatcaaat gtatcttaac
    22381 tcaagagaca atgtatatgt gaacagacat aaagatatat gtttaatgga tatagccact
    22441 gttgcagtaa aagaattaaa gaagaatggg aaattaaatg acctagatga aagtgaagaa
    22501 ataaatgcat gtagtataaa cgtagatgtt gaagttgatg gtaagatgga aaaatatcta
    22561 gtaatgttta aaaatgaaac tcacaaccat ccaactgaaa tagaaccatt tggtggagca
    22621 gctacttgtt taggtggagc tataagagac ccattatcag gaagaagtta tgtttatcaa
    22681 gcaatgcgtg ttacaggaag tgctgaccca agaacgactt tagaagatac tttacctgga
    22741 aaacttatgc aaagaaaaat aactacagaa gctgctcatg gatatagttc atatggtaac
    22801 caaataggtc ttacaactgg acaagttgca gaagtatatg atgaaaactt tgttgcaaag
    22861 agaatggaaa taggtgcagt tatagcagca gtaccaaaag agaatgtagt aagagaaaga
    22921 cctgaagcag gagatgtaat agttcttctt ggaggaaaaa caggaagaga tggttgtgga
    22981 ggggcaactg gttcatctaa agaacatagt gaagagtcaa tcttaacttg tagtgcagaa
    23041 gttcaaaaag gagatgctcc taacgaaaga aaaatacaaa gattctttag aaataaagaa
    23101 gttgctcaaa tgataaaaag atgtaatgat tttggagcag gtggagtttg tgttgctata
    23161 ggagagattg ctgatagttt agatataaac ttagacttag tacctaaaaa atacgatgga
    23221 cttgatggaa ctgagcttgc aatttcagag tcacaagagc gtatggcagt agctataaaa
    23281 aaagaaaata aagataaatt tatacaatta gcagtagaag aaaacttaga agctacacat
    23341 gtagctacag taacagatac aggatattta agaatgttct ggaatggaaa agcaattgta
    23401 gatataaata gagagttctt agatacaaac ggagtaaaac aaactactga tgtccatgtt
    23461 actaaagtag atgaagaaaa tacattcttt agctctaatg aaatagtaaa agatgttaag
    23521 tgtgcaagta tgaaagacaa gtttacaaaa gtattatctg accttaatgt ttgttctcaa
    23581 aaaggattag tagagatgtt tgataataca attggtggta atacagtact tatgccattt
    23641 ggaggaaagt atcaagcgac tccaacacaa ggtatggttg ctaagatacc agtacttgga
    23701 ggagaaacta atacttctac aataatgact tatggatata atccaaaggt aggaaaatgg
    23761 agtccattcc atggtgcctt gtacgcagta gtagaatctg tgtgtaaatt ggttgctatt
    23821 ggaggaaatt atagtacaac aagacttaca ttccaagagt attttgaaaa acttggtaac
    23881 aatccagaaa aatggggtaa accattctca gcacttttag gagcatttta tgcacaaagt
    23941 aaatttgaga tacctgctat aggtggaaaa gacagtatgt ctggtacatt taaagatatc
    24001 gaagttccac caacattagt ttcatttgct gttgatacag tagatgcgaa gaaagtagta
    24061 tctccagaat ttaaaaaagc agactcaaaa gtagtaatgt tatgtgtaaa caaggcagaa
    24121 aatgatgttg tagattttga ggaattaaag agaaacttag acaaagtaag agaacttata
    24181 catggaaata aagttttatc aacatatgca ttaggatttg caggtgtagg tgaagcaata
    24241 agtaaaatgg cttttggtaa taaaatagga tttaagttta gtgaagaagc tgaaaaaaca
    24301 tttacagatg ataaattatt tgaggctagt tatggaaata tagtacttga attagcaaat
    24361 gatgatttat gtatgttaga aggatacaat tatgttgtgc ttggaagtac aactaaagaa
    24421 gctagtatat ttatcaaagg tgaagaactt gcattagatg aattatataa agcacactgt
    24481 agtactttag agcctatatt cccaactaaa acagaagaag taaaatcaaa gatagagact
    24541 ataagttata tatcacaagg agaagctaaa aaatcatcat taagtatagc tactccaaga
    24601 gtatttatac cagcatttcc tggaacaaac tgtgaatatg actctgcaag agcatttgaa
    24661 agagtaggag ctaatgcaaa cataagagta ttcaagaatt taacttataa agacatagaa
    24721 gattctatag atacaatagt aaacgaaata aaatcttctc aaataataat gttaccaggt
    24781 ggatttagtg ctggtgatga gccagatggt tctggtaaat ttatagctac tgtatttaga
    24841 aatcctagag tgcaagaagc tataaatgaa tttttaactc aaaaagatgg actaatgtta
    24901 ggtatctgta atggattcca agtattaatt aaattaggat tagttccata tggagaaata
    24961 agagttccaa gtgaaagtgc tccaacactt acttacaata atattggtcg tcatcaagct
    25021 aagattgcta gaactagaat ttcatctaat aaatctcctt ggttagctca aactaatgtt
    25081 ggagatattc ataatatagc aatttctcat ggagaaggta aatttgttgc tagtgaagat
    25141 gttatgagag aattaattgc taatggacaa gtagcaactc aatatgttga ctttaataat
    25201 gaagctactt atgatattga gtttaatcca aatggttcat tctatgctgt tgaaggtata
    25261 actagtgctg atggtagagt gtttggtaaa atgggacatt ctgagagaat tggtgaggaa
    25321 gtatttaaga atattatagg tgaaaaagac caaaagatat ttgaatctgg agttaaatat
    25381 tttagataaa gctttgatgt ttaattgata gctagaatat ataatttaga tatttagatt
    25441 taaagagatg taataatatg atgataatca tttaatatta catctctttt ttatttatct
    25501 aagaaataat aagttcaacc taagttaaaa tacaagctgt gttataatta attactaagt
    25561 aaaattaaat atttattgga gggcaacata attaatgaaa atattaataa cagggtctaa
    25621 tggtcaatta ggaaaagaat tggttagcca attagaagca atcaatcaaa gtataaatca
    25681 accaaaatac gttatattag ctacaacaag aaatgatttg gatatatcca atcagactaa
    25741 tatagataat tttattttgc ataataaacc tgatgtaatt gtaaactgtg cagcatatac
    25801 aaaggttgat gcatgtgaag ataatatcga aattgcgtat aaaataaatg ccttaggagt
    25861 gagaaattta gcaatagcat cagaaaaagt taatgctaag cttgttcaca tatcaactga
    25921 ctatgtattc aatggttttt ctaaatatcc atatagggaa gacaataaaa ctgaacccaa
    25981 ttctgtgtat ggaaaaagta aacttatggg agaaaagttt gttgaacaat tctcacataa
    26041 gtactttata ttgagaacag catggttata tggtaatggg aataattttg taaagactat
    26101 gataaaactt tctttagaaa ataaagaggt taatgtggtt aatgaccagt ttggaacccc
    26161 aacaagtaca gtagacttag caaaaattat aataagaatc atggaaacag aacattatgg
    26221 agtttatcat gctacatgtg aaggtgagtg tagttggtat gattttgcca aaaagatatt
    26281 tgaattaaaa aatatagata taaaagtaaa tccaattaag agtaacgaat ttaaatgtaa
    26341 ggctcaaaga ccactatatt ctgtattgga taattttatg ttgaagttaa ttggtttaaa
    26401 ttcttttaga aagtgggaag aatccattga agaatattta gaaagatatg atataaatga
    26461 aaagtaaatg tattattttt agaaaagaaa ataagatatt ataaaaaata taaatatttc
    26521 ttttatttat ccgataatca taatatgaca aaaacaaatt taaggatgga aaaagatgaa
    26581 aggaattatt ttagcaggag ggtctggtac aagactatat cctataacaa aatgtatatc
    26641 aaaacaaaca cttccaatat acgataaacc aatgatatat taccctatgt cggttcttat
    26701 gttggcaggg atacaagata tattaataat atctacacca agagatataa aagcatttga
    26761 agaattattt ggagatggaa gttcactagg gcttaaaata acatatgcaa ttcaggagaa
    26821 agcaaatgga attgcagaag cgtttataat aggagaagaa tttataggaa atgacagagt
    26881 gtctcttgta ttgggggata atatatttta tggatatgga tttagtgaaa ggttggagaa
    26941 tgcagttaaa agaaaaggag caactatatt tgggtatcat gtaagtaatc caaattcgtt
    27001 tggagttgta gaatttgata aggattttaa tgtattatca atagaagaaa aacctacagt
    27061 tcctaaatct aattatgcta taccaggatt atatttttat gataatgatg ttgtagagat
    27121 agcaaagcta gttgaaccat cagatagagg tgaattagaa ataacctcta taaacagtga
    27181 atatctaaaa cgaggaaaac taaaagtgga acttctagga agaggtatgg cttggcttga
    27241 tactggtaca cataaaggtc tattggatgc ttctaactat gttgaagtgg ttcaaacaag
    27301 acagggactg tatatagcat gtttagaaga gattgcatat acaaaaggat atataagtag
    27361 agaacaatta atgaaactag ccaagccact gataaaaact gaatatggtc aatatctatt
    27421 aaaattggct agtgaagata tgcaatatgg tagataaaat ttattataaa ttttattaag
    27481 ggagtttgtt ataatgggaa aaatcaatgt tataaagact aaaatagaag gattatatat
    27541 agtcgaacca aatgtatttt gtgatgaaag aggatatttt atggaaagct ataataaaaa
    27601 ggattttata gagcagggtc ttgatatgtg ttttattcaa gataatgagt ctagctcaag
    27661 aaaaggtgtt ttaagaggaa tgcattttca ggttagatat cctcaaggaa aattagtcag
    27721 agttacagag ggagaagttt tcgatgttgc agttgattta agaaaagatt cacagacatt
    27781 tgggaaatgg gaaggtgtaa tattgtctga taagaataaa aaacaatttt atattccaga
    27841 agggtttgca catggatttt tagttttatc agaaaaagca gtatttaatt acaaatgtac
    27901 aaactattat tatccagaat ttgagcatgg gtttatatgg aatgacaagc aaatatctat
    27961 tgagtggcct ttaaaaggaa ttgaagaaat atttttatca gaaaaggaca aaaaacaaaa
    28021 aagttttaaa gatatttgta taagacgtta ataaaaattt attagaatta acatttagga
    28081 gaaaaaatat gaaaaaaata ctgataactg gaggagcagg atttattggg agtaactttg
    28141 tacattatat gcttaacaag tatgtagatt attttattgt taacctagac ttgttgactt
    28201 atgctggtaa tttagagaca ttaatagaaa tacaagacag ttccaattat aaatttgtta
    28261 aaggagatat ttcaaatagg gaacagatgt ataagctatt tgaagaagaa aggtttgata
    28321 tagttgttaa ctttgccgct gaatctcatg tagataggtc agttgagaat cctgatttat
    28381 ttattaaaac aaacattata ggaacacaag tacttttaga tgcatctata aaatatggag
    28441 ttaaaaggta tcatcaaata tctacagacg aagtgtatgg tgatttacca attgatagaa
    28501 aggatttatt ctttacagaa caatctccta tcaatccaag ttctccatat tcagcttcta
    28561 aagcatcagc agatttgttg gtaagttctt attatagaac atatggattg ttaacaacta
    28621 tatctaggtg ttccaacaat tatgggccat atcattttcc tgaaaaattg atacctctta
    28681 tgattttaaa tgctttggaa aacaaacaat tgccagtata tgggaatggg gaaaatgtaa
    28741 gagattggtt acatgtatat gaccattgta ctgctataga cttaataata cataaaggga
    28801 atatagggga gatttacaat ataggaggtc ataatgaaag aagtaattta gaagtagtaa
    28861 aaatgatatt aaatctttta gataaatctg aagagttaat atcctatgta aatgatagac
    28921 ctggtcatga tttaagatat gctattgatg caactaaaat agagaatgag ttaggttgga
    28981 aggcaaaata tgactttgat ttgggtataa aagaaactgt taagtggtat atagaaaatg
    29041 aatcttggtg gaaagctgtt ttatcagtgt aactttgaaa tttataaaat ttagaaaggg
    29101 gcgttttata ttgaatgaga taagttcact aattaatata ttagcattga ctcaattatc
    29161 tggtagtaat actacaaatc aatgtaactg tacatataat agttcatctg gatttgatat
    29221 gattatgatg accttgttaa aagcattatc acaaaataat caacagactt ctaatggcta
    29281 caatttatcg tctaattctg agaatttatt tgacgaactc ggcagtgtag taaataatac
    29341 agctagtaga tttatagatg ttgatacaaa agataaaagt gtaaaatcaa gaattgaaaa
    29401 tgctatagag caagcatcaa aaaaatataa tgtggattcg aatcttataa aagcaataat
    29461 taaagttgaa tcggatttta atccaaatac tgtttcttct gctggtgcta agggtcttat
    29521 gcaacttatg ccagaaaatt gcagagactt gggggttaca gaccctttta atatagagca
    29581 gaatatagat gctggaacta gacatattaa ggaatacatt gatatgtttg gagggagcat
    29641 agaaatggga ttgatggcat ataatggtgg tcctggaaga atgaaaagta gaggagtaga
    29701 atcaatctct gacctgtata aaatgccaaa agaaacacaa aattatattc ctaaggttat
    29761 gaaatattat agagggtagg ctttattatg gggataaata tagataagct aaacataagt
    29821 ccaagtataa atatggaggt taaaaaacta aaagagagta ataaattttc tgacaaatta
    29881 aactctttaa aacaatcttc ttcattggaa gatataaaac gaaatttgga aagtgtaaaa
    29941 aaggcaggag aaaggcttgt acttactcaa aactatggcg acattagtat atataaaaat
    30001 gcagtaaaag aatacctaaa atctgttgta gataatatgt attcacttga taaaaatagt
    30061 agcttttggg agcatcagta ttataagaat gtaaagattg ttgatagtaa acttgaggat
    30121 atgactagta agttaattag tgaagaaaaa gaaaacatag atattgtttc tactgttgat
    30181 atgatacaag gcttgttgat agatatgtat agataaggtt atacaaggag aaaagtttta
    30241 tgaatagtaa aaaagaggat atatatgaat tgaattttgg gcaattaata gaagaaaaat
    30301 ctggtgttat agaagaaaat caaaatgttt ttaatgcaaa agtaaatgta gctgtttcta
    30361 taggaaatac aacagaaaca ataaaaaata tattagattt aaaagaaggc tctataataa
    30421 aactaagtaa gaatgtggat gaaaaattag atatatatgc aaatgataga ttgtttgctt
    30481 atggagaaag cattattaca aatgagaagt tatctgtaag attatctaaa atagaagatt
    30541 cagatgtaaa ttagtatttt aaatagtatc cctttaggct atgatgtatg agtttagcta
    30601 aggggatatt tttataaaaa atttaaaata tgcgtgaatt atataaatgt ttttatttta
    30661 tgagcgataa ttaaattata agtatgtaat tttggaggtg aaactatgga tattaaaaat
    30721 gtaagttcaa atattgtagt gaataattat ttaaagaata catctggtgt aaaaaaagtg
    30781 gaagaagtta aaaaaattaa taaaacagac aagatagagc tatctaaatt tgcaaaggaa
    30841 atggcaagtg tgtcaaatat gagtgatgag aatattagga ataaaaaagt cgatgaaata
    30901 aaaaatgcat tagaaaatgg aacttacaaa ataaaaccaa aagaaattgc gaaaaaaatg
    30961 atagaggata tgcgaggata aagtcatgat tacagactta aaagttataa tatatgaaga
    31021 aaaaaatata attagtaaga tgatggaatt attaaaagaa caatttgatt atatagttga
    31081 aaaagactta gaaaatttaa atatgttaaa tcctaaacta gaagatatta gcagggagct
    31141 tgcatccata gaaataaaga gaagacaact ttttggtgat gatatttcaa tgagtgaagt
    31201 tgttgaaaat agtaatgata gatatttaaa agaaatatat acagacttga aacacattct
    31261 tgctttagct aaaaatcaac aagaatcaaa tgattctcta ataaagaaag aacttatttt
    31321 tacgaaaaaa atgattaatt tcataaaacc agctgataag caaactgcaa cttataattc
    31381 ttatggaaat attagaaaat aaatattagg aggagaaata tggcaggatt attttccatt
    31441 ttaaatacag ctaattcagg aatgaatgcg caccaaaaag caatacagac aatttctcat
    31501 aatataagta atctagatac agacggttat tctaggcaaa ggactgagtt tgctacaaat
    31561 agtccaatgt atatgcctag tttgagtaat tcaattgcaa gaggacaatt agggactgga
    31621 gtacacgtaa cggatgtaac aagagctaga aatagttttt atgattacca atttagagca
    31681 gaagctcata agtatggtaa tacagtttca aagtatgact attataatac tatagaaact
    31741 ataataaatg aaccatcaga ctatggaata tctgcaggta tagacgactt ttttaaagga
    31801 tgggaatcat tagcaaagga tataaatagt gatagtaaaa aaagtctagt ggtagaaaat
    31861 gcatcaaatc tagcaacttt aataagtgaa agttataaga agcttaataa gttaaaggga
    31921 gatatagacg ataatataaa ctctgaaata aaacagataa atgacatgtt aaaaagtctt
    31981 gaagatttga ataaatctat tgatgttata tctggttctg gctctactcc aaatgattta
    32041 cttgatgaaa gagatagaat attagatgac ttaagcttta aattagacct tgaaaatagc
    32101 gatgttaaaa atatgctttc agatggtaaa ttagaactta atgaattaaa aaatgcagat
    32161 ggaacttgga aaacaggtgt atcaggaacc ttacaaggct tgtttgaaat gcatggtaag
    32221 attgatacat ataaaagcga tttaaaagat gtatctgatg ggttagctaa gcagataaat
    32281 gatgtttata atacatctgc tggaataaca gtaacagatt tttttataac ttcaaatgta
    32341 gatggtgaag atatcataaa ggtaaatcca gctataaaga gtaacccaaa tgaattaaaa
    32401 cttactgctg atgaggcttc aaagattgct aagttaaagg atgaaaaaat agatattggg
    32461 gtggctggtg gaaaagtaag tacaataagt gaccactata aagcctttgc agaatcagta
    32521 ggtttagatt ctcaaaaagt gaatcaagat gaagtgaatc aacgtaaaat tataaataat
    32581 attgataact cacgaatgag tgtttcaggc gtatctttgg atgaagaaat gacagagctt
    32641 ataaaaatac aacgctccta tcaagctagt gcaaaagtta tgtcaacagc tgtgcaactg
    32701 ttggatgttg taataaatgg aataatttaa tagaaggggg aatttataat gagagttagt
    32761 acaggtatga tgtctagcaa ctatctaaac tcactacaag ataatctaca aagattagat
    32821 aaggttaata gacaagttaa tacaacgaaa gaaataaaca agctatcaga taatccatat
    32881 aaggcaataa aaatattgaa ctcaaaaagt gaaataaaaa ctatggagac ttatgttgag
    32941 aattgtaaag atacagggaa ttggttagaa actactgata ctgcattaga tcagcttgga
    33001 aaattattat cggacataaa gaaaactttg gtggcaagtg gtgatggtgc atattcagat
    33061 gatgagctaa agactcttag taatgaaact aatgaaaaga tgaaagaaat tgcaaatgcc
    33121 ttaaatgcaa cacatgaagg aaagtacata ttttcaggtt caaatacagg tgtaccacct
    33181 gttgaatgta tagataatgc tgacggttca gtgagcttaa agtttaattc tagtttaaat
    33241 cttaataaat taaatgactc tttgagtatg gatatagctc aaggaataag tgtagattat
    33301 aatgttaaat tgagtagcct tggatttgac ccaacaaaga ctgacccatt tgaagagtta
    33361 aataatatat caaagaaatt agcaaatcca gatgctgatg ctataaaaga gctgacaaca
    33421 acttgtttag gcgacatgga acaattaata gaaaatacag taaatgttag gtctatatat
    33481 gggacaaaag caaatacagt agaggctatg aaggaaaaaa atgatgatag tttgatacag
    33541 ttaaaggata ttttgtcaca aaatgaagaa atagattatg gagaaaaact cgttcaatta
    33601 aaggcagcag agttaactta tcaagcttca ctacaaacag gaggaaagtt atttaatgta
    33661 tctatattag attacatata gtaaaataac gcagatatga tgaaggttac attaaaaaaa
    33721 ggtatattag gttttgaaaa tttaaaagaa tatgagcttt tagatataga agataatgat
    33781 tctttaaaag aatttaattc tactgaagaa gaaggtatag gatttgttgt tgtatctcca
    33841 tttgagatta tcaatgaata tgaaatagtt ttaaatcagg aaacaataga aaaactagaa
    33901 gtgaaatctc caaaggatgt tgtactattt aatataatta ctcttggtca gacatttgaa
    33961 gaatcaactg tgaacatgaa agctcctata gtgataaata taaaaactaa ttttggaatg
    34021 caaataattt tacaggatga taaatattca ataaggcatc cattattgag aggtgataat
    34081 ggatgctagt aatttcaaga aaaaaagatg aagcagtact tataggagaa agcatagaag
    34141 taaaaattgt aggggtagat ggaaacaatg tgaaactagc tatatcggcg cctaacaata
    34201 taagtatatt aagaaaagaa atatacgaaa aagtaaagag tgagaatata aaagctacaa
    34261 ataaaaatat aaaaatttta aagtcattaa aataataagc ttggaggaag taatatatgt
    34321 atggagaaaa tccttataat tcttataaac aaaatgctgt ttttatggca tcaaaggaac
    34381 aattgttact tatgcttgtt gatggagcag taaaatatac caaaatagcc agagcagcta
    34441 tcttagataa aaatacacag aaagcgcata gagaacttgt aagagttcag gatatattta
    34501 cagaactaat ggttacatta gaccaaaatg caggacaatg ggcaaaggat atgtacaaag
    34561 tttatgattt tgtaagatat gaactttcaa gagccaatat tagaaaagac atccaggtaa
    34621 tagataatgt gttgcctgta atagaacaaa ttaaagacac atggcatgaa gctgataaaa
    34681 aaagtaaaga agaaagaagt aggtacaaat aatggaatta aatttagctg aattagataa
    34741 gttatcaaaa gaggaattac tattgatgct tgtagatggc acagtaaaat ataccaatat
    34801 atccaaagaa gctcttttaa ataacgacta tttgaaagca cataatgaac ttataagagt
    34861 tcaaaatata tttacagaac taatgactac attagaccaa gatgcaggtc aatgggcaaa
    34921 ggatatgtat aaagtttatg aatttataaa atctgaatta gcaatagctg atgaaaataa
    34981 agatattaaa ataattgatg atatattgcc aatagtaaaa caaattagag atacatggca
    35041 tgaagtttac aatcaactaa aaatataatt agatggaggg ataaaggtat gtcaagtata
    35101 agtcctataa gagttacagg tctttcagga aattttgata tggaaggcat aatcgaagct
    35161 agtatgatta gagacaagga aaaagttgat aaagcaaaac aagaacaaca aatcgttaaa
    35221 tggaagcaag aaatatatag aaatgttata caagaatcaa aagatcttta tgataaatat
    35281 ctaagcgtaa attctcctaa tagtatagta agtgaaaaag catactcttc tacaagaata
    35341 accagttctg atgaaagtat tatagtagca aaaggctcag ctggtgcaga aaaaataaat
    35401 tatcaatttg cagtttctca aatggctgaa ccagcaaaat ttactattaa attaaattca
    35461 agtgaaccta ttgttcgaca gttccctcca aatgccagtg gagctagttc tttaactata
    35521 ggagatgtaa atataccaat atctgaacaa gatactacaa gtactattgt aagtaagata
    35581 aactcccttt gcgcagataa tgatataaag gcttcttata gtgagatgac aggtgaattg
    35641 attatttcga gaaaacaaac tggttcgtca tcagacatta atttaaaagt aattggaaat
    35701 gacaatttag ctcagcaaat tgctaatgat aatggtatca catttgcaaa tgatgctagt
    35761 ggaaacaaag tggcaagtgt atatggaaaa aatctagaag ctgatgtaac tgatgaacat
    35821 ggaagagtaa ctcatataag taaagaacaa aattcattta atatagataa tattgactat
    35881 aatgtaaatt caaaaggaac tgcaaagttg acttctgtca ctgatactga agaagctgtt
    35941 aaaaatatgc aagcatttgt ggatgattat aataaactga tggacaaggt ctatggttta
    36001 gttactacta aaaaaccaaa agattatccg cctcttacag atgcccaaaa agaagatatg
    36061 acaactgaag aaatagaaaa atgggaaaag aaagctaaag aaggtatact tagaaatgat
    36121 gatgagttaa gaggttttgt tgaagatatt cagtctgcat tttttggaga tggaaaaaat
    36181 attattgcat taagaaaact aggtatcaat gaaagcgaaa attacaataa aaaaggtcaa
    36241 atatcattta atgcagatac tttttcaaag gctcttatag atgatagtga taaggtatac
    36301 aaaacactag caggttattc ttcgaattat gatgataagg gaatgtttga aaagctaaaa
    36361 gatattgtat atgaatattc tggaagttca acttctaaac ttcctaaaaa agcaggtata
    36421 gaaaaaactg cttctgctag tgaaaatgta tattcaaaac aaattgcaga gcaagaaaga
    36481 aatataagca ggttagttga aaaaatgaat gataaagaga aaagacttta tgctaaatat
    36541 tcagccttag aatctttgtt gaatcagtat tcttcccaaa tgaattattt ctcacaagca
    36601 cagggtaatt aattatggat aaattaaaag aaaaattaaa tttatataaa gatatatctt
    36661 tacaaattat aaatttgatt gaaaaggaag aatatataaa gataagtagt aagttaggtg
    36721 aaagacaaga gattataaat agtgtaagtg aaattgatag aaatgatttt atacagcttt
    36781 ataatagaat ggaattaatt gaaattgata gtaggataag agatatttta caaggacaat
    36841 tattagaagt aaaaaaagaa ttacatgagt ataagcttac taaacaagtg aacacaatgt
    36901 attataactt aaatagagaa aaagtaaata tttttaataa aaaagtataa agttatgtac
    36961 catcttgtcc gattatataa atgaagaatt aataaaaaag attaaaagta gaaatgacaa
    37021 ggatgtcaac tatactaagg agggtaaaat aatgagagtt aatacaaatg taagtgcttt
    37081 gatagcaaat aaccaaatgg gaagaaacgt aaatgcacaa agtaagtcta tggagaagtt
    37141 atcttctggt gttagaatta agagagctgc tgatgatgct gctgggcttg ctatatctga
    37201 gaaaatgaga gctcaaataa aaggtttaga ccaagcagga agaaacgttc aagatggtat
    37261 atctgttgta caaacagcag aaggtgcatt agaagaaact ggtaatatac tacaaagaat
    37321 gagaacttta tcagtacaat cttcaaatga aactaatact gctgaagaaa gacaaaagat
    37381 agctgatgag ttgttacaat taaaggatga agttgaaaga atatcaagct caattgagtt
    37441 taatggtaag aaattacttg atggaagttc tacagaaata agattacaag ttggagctaa
    37501 ttttggaaca aatgttgcag gaacaactaa caacaacaat gaaataaaag ttgctctagt
    37561 aaatacttca agtataatga gtaaagcagg tataacaagt tctacaatag caagcttaaa
    37621 tgcagatgga acttctggaa cagatgcagc taaacaaatg gtgtctagct tagatgtagc
    37681 tcttaaagaa ttaaatacat caagagcaaa attaggggca caacaaaata gactagaatc
    37741 aactcaaaac aacttaaata atactataga aaatgttaca gcagctgaat caagaataag
    37801 agatacagat gttgcttcag aaatggttaa cttatctaaa atgaatatat tagttcaagc
    37861 atcacagtca atgcttgctc aagctaatca gcaaccacaa ggagttttac aattattagg
    37921 ataaaataat tttgtatatt ggatatagaa gaaagggtaa ggctttgctt tactcctttc
    37981 tttttttaca aatatacttt tgaaaggaaa atatatatgt tacttagtat agttatgatt
    38041 gttaaaaatg aagaaaaaat acttgaaaaa actttaaaat cattaaccac attaagaagt
    38101 tctatagaga gtgaacttat aatagttgat acaggttcta cagatgatac tataaatata
    38161 tctaggaaat acacggaaaa tgtgtacttt cataattgga ataatgattt ttctagtatg
    38221 agaaatatat caatatccta tgctaaaggt gaatggttac ttatattaga tgcagatgaa
    38281 atactaattg attcttcaac tattgtgaaa ttttttaaag atggtcttga taaaaaattt
    38341 aattcggcct caatacaatt aaaaaatctt tactcatatg ataaaaaatt atatggatat
    38401 tgctcagtgc ttagactttt taaaaacatc gattttagat accatggtaa agtacatgaa
    38461 caaccaatat acaaaaaccc tatatttaat aatgttgctg attttgaaca ttatggatat
    38521 ttgtttgaag atgaagaaat aagattaaat aaagtaaaaa gaaatgaaaa actgttattt
    38581 gaagaattaa aagagaatga taattctcca tatacaaatt atcaattagg gaaaaatttt
    38641 attatattag gaaaatatca tgaggcttta tactatcttg agaaatctta tgaattatat
    38701 tctaaactag aatcggcacc tggttacttg ataacaagtc ttgtaaaaac atatttatat
    38761 ttaggaaagc ataagaagtg tgaaaaattg tgtctaaagt atattaagaa agacatgaat
    38821 aatattgata tatattatta tattgcacaa gcacaagtag atttagggaa gtatgaaaat
    38881 agtatagatt cgtataagag atatatttat ttgttagata attatgaaat aagtacacaa
    38941 gcaaatagtt tattttcaga cactgatact gtagggctta gggatgaagc tattattaca
    39001 ctgataaaga tttattataa acttgaaaaa tatgagtctg taatttcaga gtacaataac
    39061 attgaggata tggaaaagaa gaagaatgtg tactttagct tatttatgtc cctatacaaa
    39121 ttgaacagat ttgaagatat aaaaaactat tataaagaag tttcaaattc taaaattgaa
    39181 aaaaatagtt tttataaaaa tatagaagaa gttataaata atattaaaga tgatgaaaaa
    39241 gagcatgtat ataaaatcct ttgtgacatt gaaggtaatt atggcatttt aaatcagata
    39301 aggataaata aatgtgtatc tatagataag tgtaaagaaa tactcaatga agaaaaagaa
    39361 ataatttatg catatattat aaaagtagct tttgaagata agattgattt actagatatt
    39421 ttttatgaaa tggaatatgt atggatagag aaatacctaa aatatctttt agcactagaa
    39481 agaagtttta atttagtact atataagtac ataattagtc gtccgaatac atcagatata
    39541 aaaaagataa gagtatataa agctatatct aaagtagttt tagaaagtat aagtttaagt
    39601 gatgaaaaat ataaggagat tttttatctt tatataatgt attcttataa gtacataaga
    39661 tacatatata gaaatttaag cgattttgaa ttaataaaat ttgtttgcaa tgatttagat
    39721 aagtttacta tagaatttaa gcatctactt gacattaaaa ataaagtaaa tgatgctaat
    39781 gaaaaattag agtatataca tagtatgagg aaattactaa atgagtatcc tttttataat
    39841 aaaataataa aaatacttat aagtgaatta gaagaaaata taaaagaaag tgaagaattt
    39901 aaggatttaa aagatagttt tattattagt atagaaaata tgattgaaac tggcaaaata
    39961 aatgatgcga aatcattggt agatgactat tcaaaaacat ttatggatga tgtaaaaatt
    40021 ttaaatataa agggaatttt atatatgttt gaaaataagc ttgataaagc tgattttatg
    40081 ttaaaaaagg cattatcatt agatgtagaa aatgaagata ctatatataa tatagaatac
    40141 ttaaaaagtt taagataagt caaaaatggg agaattgaag aatgaataca ccaatagtgg
    40201 ttaatgaaaa atctaaagat ataataaaag attggcaata tacgtataca aacgaagata
    40261 taaaatttac aagcataata atattgacat ataatcaaat agaatatact aaattatgta
    40321 ttgaaagtat taggaagttt accccaaaga ataaatatga aataataata attgacaata
    40381 actcttctga tggaacagtt gagtggttaa agggacaatc tgacataaaa tctgtatata
    40441 ataacaaaaa cctaggattt ccaaaaggat gcaatcaagg tattgagttg gctactggtg
    40501 aaaacatatt gttgttaaat aatgatgtaa tagttactcc aaattggctt tataatttga
    40561 ataaagcttt atggtctcaa gataatatag gtgctgtagg tcctattaca aatgcatgtt
    40621 cgtactatca acaaattgat gttgattata aatcaattga ggatatgttt atgtttgcac
    40681 acaaaataaa tacatctaat attaacctat ggaaatatag attaaagtta gttggatttt
    40741 gcatgttaat aaaaaaatct gtgctggata atgtaggggt tttagatgaa ttattcacac
    40801 ctggaaattt tgaagatgat gatttatctt ttaggatgct agagtcagga tataagctat
    40861 tattatgcca ggatactttt atacaccatt ttggaagtgt atcttttaaa gaaaatcgta
    40921 ataaatatac agatataatg tatattaata gaagaaaatt tgaaactaag tggggattta
    40981 atccagaata tagtggaatg ataagaacag atttgatttc taaaatagat gatagtttgg
    41041 aggcaaatat taatgtttta gaaataggct gtggtatagg ggctactctt ttagaaataa
    41101 aaaatatata taaaaatgca tcaatatatg gaatagaaat tagtgagtca gcaggtaaac
    41161 ttgcaaagta tagtgcagat gtacttatag cagatataga aaaagtaaag ttggattatg
    41221 aaaaagattt ttttgattat ataatattgg gagatgtact tgaacattta actgacccat
    41281 ggaagatagt caatgaactg aaacaatatc taaagaagga tggttgtttg atatctagta
    41341 taccaaatat tatgcatgtt tctgttttaa gaaaccttgt gttaggtaat tttacatatg
    41401 aagattcagg aattttagac aagactcatt taaggttttt tacattaaat gagataacaa
    41461 aaatgtttaa tgatagtaat tacataataa atagtgttac agctactagt atgccacttt
    41521 cggaaattga taatagattc attgatacaa tgtgtaagga aagctgtaaa gaaatgagac
    41581 aacagtacga atcttatcaa tatatagtaa aagccactaa aagcatagat actaaaagat
    41641 attctagtat tagtatggtt aatttaaaat acgctttaat gagaattgat aatgaattag
    41701 atgtagatag taatttaagt tatatatttg ataattataa tataactgaa ccatgttttg
    41761 ttgatgatat tgaatattta gttgatacgt ttgtgataaa taaagcaact ctattaaata
    41821 gattaggagt tgaggcattt aacagaggtt taggagattt tagtataaac atgtttatga
    41881 cagtattaga gatggataga gatgatattg attcaatata taatataaca agtgtattat
    41941 gtgaattaga agaatttgat actgcttaca acttaattaa aaatagtagc aaaaaagtac
    42001 agagtgaaag tgacatacaa gatatacttt attttattga ggggaagaga tatgaataat
    42061 gataagatat gttttataac ctgtgtaaat gatgaaagac tttatgagga atgtcttgta
    42121 tatataaata gcctagacat tcctaaaaac tttagcatag aaacagtagc tttaagagga
    42181 gccgattcta ttgcctctgc ttataataaa gctatgagag aaacagattc taagtataag
    42241 gtatacttac atcaagatgc ttttataatt aataaaaatt tcatatatga cattttagag
    42301 atatttaaaa aaaatacaga tgtaggaatg attggtgtag ctggagtaaa agatatacct
    42361 gtgaatggaa tttggtggga ttctgataaa aaagcaggga aagtatatga tagccataca
    42421 ggtaaaatgg atatttatga atttagtaac tttgaagaat gttttaatga agttatggct
    42481 atagatggat taattatgat tactcaatat gatgtatttt ggagagagga taagtttgat
    42541 gggtggcatt tttatgattt gtcacagagt atggaattta gacaaaataa atacaaagtt
    42601 attgtaccaa aacaaaataa gccttggtgt atgcatgatt ctggagtagc aaatataaat
    42661 aatggatatg atgaatatag gataaaattt gtaaaagagt acataaatct tgatttatat
    42721 agagaaaatt tttatgaatt tgataagaag aaagcaagta ccatacttaa gagtaataag
    42781 catgtaatgg attgtatgaa agaggagctt acagaacaat taagattaaa gaattataag
    42841 gatgttttag atattgtttc taaaattgct catttttctg tttttaatca tactggcttg
    42901 tattcttgtg aaaaatgtga gagagcattg ctagaatgtg caaataattt accagaaata
    42961 gaaaatacga aattatttac aaaaaaagat ttaaaaagaa gaaaagtatt acatgtttta
    43021 agtgaaggat attcaacagg agggcataca agactagtta aaaattggat tgagattgac
    43081 aaagattctg ttcattcagt tgtaactaca tggcaatcag atacactgcc agactggtta
    43141 ttaaaagcaa tagatgatag tggtggcgaa gttatatctt tggatttaat ttcagatgat
    43201 tatttaaaaa gagctgtagc tttgaggaaa atagcttatg actgggctga tatagttatt
    43261 ttatatgttc atatgtggga tacaatacct gttgttgctt ttggagttga tgggaaaata
    43321 cctattttac atgttaatca ctatgatagt actttttggt tagggacaag tattgcagat
    43381 attgtcattg attttagcga agctagtcaa ttaattacat tagataaaag aggcatagat
    43441 aaatctgaaa tattaccaat gccattaaaa gaaaaagaag tattttcaaa atctgaagtt
    43501 agaaaacgat ataatttgga ggatgatgtg acaataatat tgactattgc tagtgaaaga
    43561 aaatacaata gtgtaaatga tattaattac tttaaaattg ctaaaacatt gattgaaaat
    43621 acagataata caattattat tgttgtagga gttgatacag agaatctcta ttggaaaact
    43681 ttaagtaacg ttacagataa tagagttata tgttttggaa caacagcaaa tatagacgat
    43741 ttttataaga tttcggatat atacctagag tctttcatgg tagggagtca tacttcaaaa
    43801 ttagatgcaa ttttatatga tttaattcct gttaagttta aaaatgaagt ttcaccaatt
    43861 tttacaaata tgtggaattt tatagataag tttgattata agaatataaa agaggttata
    43921 gacatgataa gaaggtacaa tactagtgaa tttgacaggg ataagataaa agaaaaacaa
    43981 tcagaaataa aacattttgt tatagataaa cactgtattg aaataaaaaa ctatatagac
    44041 aaaatatata aagaggctaa tcatcataaa gttaagtatg atttatataa aaatgatgaa
    44101 atcgaagact atcagctttt tgttgcatta tatggttata tggtaaaaaa taaaaacatg
    44161 tcataggtga taaaatgaac aatattcata tcaataaatt tgaaaaaagc tgtgaaaaaa
    44221 actgggatta ttttgtagaa aataaatcta taaatggtac atttctgcaa actagaaatt
    44281 tccttaatta tcatgaagaa ggaaaattca ctgaccactc attaataata tataaaggtg
    44341 aagaaatact agcagtcgta cctgcatgtg aaacttatga aaatggtgaa agagtatttt
    44401 tttctcattt aggaagcact tttggaggta ttataataag aagtggtttt aatgatatta
    44461 ggcatgtaaa tgctattaca aataagttag aaacatattt acaggaacaa agtttcaata
    44521 aagtatattt aaaaaataca tcaagaatat tttgtaaaga ggatacttca ttactagaat
    44581 attttctatt taagaataat tacatgtttt atgatgaatt aagtatgttt ttagaattaa
    44641 gtaagtataa aaaagatata ttagctaatt ttagatatag taaaagaaga tattttaaat
    44701 attctttgaa gaataatctt acctttaaaa gattgtatag tgatgaggat atagtttgtt
    44761 tttttaattt actttctaat tgtttagata gtaaaggagc taaagtaata cattcagtag
    44821 aagaactttt ggagtttaaa aataatagat taaaagatat tgtagaattt tatgcagtat
    44881 atattgatga cattatgatt tgtgggagca tggtatttaa ctttcaagaa agagtttatc
    44941 atactcaata tctagcatca aattcgacat ataataaata ttatccaatg atatttttat
    45001 actataatct aataaaagat gctttagaga gaaattttaa gtatctttct tttggaatta
    45061 gcactctaaa tagaggcaaa tacttagacg agggtttagc tacatttaaa gaaggttttg
    45121 gttcaagtgc aatttgcaac agaacatact ataaaaatat aaataagtga taatcatgtt
    45181 ttatagagtt tttagagtat agtaaatatc tagtactcta atattacata aaaataatta
    45241 gtttataaag aaggtttgta tatgaaaaga ttaatgattt taggctcaat taagtatttt
    45301 gagaatattg ttatgtcagc taaaaaagag ggaatataca caattgtttg tgataataga
    45361 attgatactc ctgcgaaaaa gatttgtgat gaagcaatag atgttgatgt atttgatttt
    45421 gataaaataa aaagtatagc tatagataaa aagatagatg gaattttaac tggatttaca
    45481 gattctttaa tgaaaccata tgtttatatt gcaaatgaac taaatttacc atgtgtgatt
    45541 tcttgcaatc aacttgaatc tgtaactaat aaggctatta tgaaaaaata ttttaaaaat
    45601 aatgcaatac ctacaactga ttattgtatt attgaaagtc taaaagattt agaaaaaata
    45661 caaggattaa attttccact tgtaataaaa cctgttgatg gttatggttc aagaggtgta
    45721 tattttgtat caaatataga agaattaata gaaaaatttg aatattctaa ggaagcctca
    45781 caagatggac aagtaataat tgaggaattt tatgaaagtg aggaaataca aggactagct
    45841 tgggttcata atggagagtc acatgtattt tatattggag atagagagct tgtaaatata
    45901 catacaggaa gagctggaaa accagataga ttactttatc catcaaagta ttgttttcaa
    45961 tatgaggaag aaataaaatc tatatatcaa aatataacac aggcttttga tataaaaaat
    46021 ggacctcttt atgtacaaat gttagtggga gcatatggaa taaaggtatc tgaagtaatg
    46081 ccaagattac ctggaggttg tgactatatg gctatttctc aaataacaga tttagacata
    46141 ggaagtttat ttgcaaactt tagtgtgaat aatgaagtaa attttgatga aataaagaaa
    46201 tataatatgc atttatcaaa atgtgtatat gcattaccaa tatacataag acctggaaca
    46261 ataaaaaaga taaataatat agataaaata gaaaacttag attatgtcac tcaatttcta
    46321 ttattagtaa aagaaggaga tacaatagga ttatatgagg atgagagaca ggattgtgga
    46381 agagtttatg gaatagcaaa taatatattt gatgcaaata taaagaagaa atgtattcat
    46441 gaaatgatag aaatatttga tgaaaataat gaaaatatga tagaaaattt ttaaataagg
    46501 gagtatgata gatatgaagc taataactca tgaacaaatt atgtcattaa acatttctca
    46561 gaaacaagga tatgaatgga taaaagaatc ttttttaaca aaaaaagatt gtcaattacc
    46621 accaaaaata agcatggtag aagaaggtca tgtattttac aatgtaatgc cgtccttaaa
    46681 tcataaccat atgattggtg gaataaaaat ggttacaaga ttgccaggga gaaatccaag
    46741 tttaaattca gatatatttc tatacaatat gaaagatgct aaattaaagg ctattttgga
    46801 tggtaattat attacagctt ttagaactgc tgctgttgct ggtttggcta ttgatacttt
    46861 tgctaaaaaa gattttaata ctatttctat tatgggttta ggtgtaactg cttctgctat
    46921 aatgaatatg ctttcgtata ctcttaaaga taaaaatata aatataaagt tacttaaata
    46981 taaagaccaa gcagagtcat ttataaaaaa gtatgaaaaa aatgaacaat ttagttttga
    47041 aatatgtgat acacatgaag aacttattgt agattcagat gtagttattt ctactgtgac
    47101 atatacagat gaatatttat cagaatttaa atggtttaaa aaaggtgctt tattattacc
    47161 tgtacatgtt atgggatttg gaaatgttga ttatctattt gataaagtat atttagatga
    47221 ttataatcat ttgaaacatc tagaaaatat agatagatat aaatattctt gtgaatttta
    47281 taatgtttta agtaaacaag ctaaaggaag agaaaatgat gatgagagaa taatatctta
    47341 caatataggt ttgtcaatac atgatatatt atttgcaaac aaaatttacg aattgtgtga
    47401 aacactaaat tgtgaagaga tagaattaaa agaaccaaaa gaaaaatatt ggatatagtt
    47461 tatttcattc atatttacaa taatttattg taaatatgaa tatgaagtaa tatattgtta
    47521 gaaaattagc atgaatagga tagataaatt tatggataaa tataatttaa ttaagttctt
    47581 atctattgga aacgaatata taggaaattt agttgcatta gaatcaaata aagcaatacc
    47641 ttttgatata aagagagttt attacatata tgatgtacca aacaatgtaa agagaggatt
    47701 tcatgcacac aaaaacttgg aacaagtact agtttgccta aatggaagtg tgaagataaa
    47761 atgttatgat ggtgtaaaag agagtatatt taatttacat aaaaatgatg ttggattgta
    47821 tataggaacg ggagtgtggc atgagatgtt tgactttgat gaaaaaactg ttttaatttc
    47881 tatagcatct gaacattaca atgaagaaga ttatattaga gactataaag aatttttaaa
    47941 gtatttaaat aatatgtagt tattatacag ttagtgatgt attttcttaa ttaaataaat
    48001 ttaagatagc catatagaga ataaatagat taggatgtga atttattgtt ggttaaattt
    48061 aataatttat taaaacagtt ccaaactcat aaagaagaat atgaacagaa gattaaggat
    48121 gtaatggaaa gtggatatta tatccttgga aatgaagtaa ggcattttga agaagaattt
    48181 gctagctatg taggtagcaa atattgtgtt ggtttagcca gtggtttaga tgcacttata
    48241 attgctttta gagcattagg aataagtgaa ggtgatgaag taatcgtgca atcaaataca
    48301 tatattgcta gtgttatggg aatcagcata aatggagcaa caccagtatt tgtagaacca
    48361 aatgaatatt ataatataga ttcatctaag atagaagaaa agataaatag caaaacaaaa
    48421 gctatattag tagttcattt atatggtcaa gcaagtaaaa tgaaagatat acaagaatta
    48481 tgtgataagt ataacttgta tttaattgaa gattgtgctc aatctcatgg agctaaatat
    48541 gatggaaaaa tgacaggaac atttggtgat ataggttgtt ttagttttta tccaagtaaa
    48601 aatttgggtg catttggaga tgcaggagct atagtaacta gtaatgagga aatagctgat
    48661 aaagtgaaaa ttcttagaaa ctatggttca gaaaagcgat attataataa atttgtcgga
    48721 tataattcaa gattggacga aattcaggca ggtttattaa gagtaaaact taaatatcta
    48781 aatgaaattg aaattgaaaa atcaaaaatc gtagatagat atcttaatga aattaataat
    48841 cccaaagtca tacttccaaa gatagaaaat aattgtactc atgtatggca cttatttgtt
    48901 ttacaagttg aaaaacgaga tacatttatg gaatacttaa agaaaaatgg tatagagtct
    48961 ataatacatt atccgatacc accacatcta tctgaagcat atgcttattt gggatataaa
    49021 aaggggagtt ttaatatagc tgagaactat tcagataaag ttgttagtat acctttattt
    49081 gtcggtatgg aagatgaaga agttagttat ataattaaaa ttataaatga atattaaact
    49141 aatcataata atatcaaaaa taaattttct atatatcatt tatttaaatt tcgttaattg
    49201 acatttttaa atattgatat attgtacaaa taaaattgaa atatatggaa gaatatagaa
    49261 taaagatgag attt
//
LOCUS       DAGQWR010000007        47980 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.007, whole genome shotgun sequence.
ACCESSION   DAGQWR010000007 DAGQWR010000000
VERSION     DAGQWR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.007"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(252..1643)
                     /locus_tag="MIW95_000309"
     CDS             complement(252..1643)
                     /locus_tag="MIW95_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBY3149669.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(1654..2874)
                     /locus_tag="MIW95_000310"
     CDS             complement(1654..2874)
                     /locus_tag="MIW95_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBY3149670.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2888..3676)
                     /locus_tag="MIW95_000311"
     CDS             complement(2888..3676)
                     /locus_tag="MIW95_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBY3149671.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3693..4967)
                     /gene="larA"
                     /locus_tag="MIW95_000312"
     CDS             complement(3693..4967)
                     /gene="larA"
                     /locus_tag="MIW95_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBY3149672.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(5085..5798)
                     /locus_tag="MIW95_000313"
     CDS             complement(5085..5798)
                     /locus_tag="MIW95_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBY3149673.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5923..7182
                     /locus_tag="MIW95_000314"
     CDS             5923..7182
                     /locus_tag="MIW95_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBY3149674.1"
                     /translation="MKKNKIIAFFAPAIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKHEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFIKKFSRKK"
     gene            7450..8016
                     /locus_tag="MIW95_000315"
     CDS             7450..8016
                     /locus_tag="MIW95_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBY3149675.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(8217..9422)
                     /locus_tag="MIW95_000316"
     CDS             complement(8217..9422)
                     /locus_tag="MIW95_000316"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBY3149676.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9525..9631)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9723..10091)
                     /locus_tag="MIW95_000317"
     CDS             complement(9723..10091)
                     /locus_tag="MIW95_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBY3149677.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(10143..11420)
                     /gene="obgE"
                     /locus_tag="MIW95_000318"
     CDS             complement(10143..11420)
                     /gene="obgE"
                     /locus_tag="MIW95_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBY3149678.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11531..11821)
                     /gene="rpmA"
                     /locus_tag="MIW95_000319"
     CDS             complement(11531..11821)
                     /gene="rpmA"
                     /locus_tag="MIW95_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBY3149679.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11835..12152)
                     /locus_tag="MIW95_000320"
     CDS             complement(11835..12152)
                     /locus_tag="MIW95_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBY3149680.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(12167..12478)
                     /gene="rplU"
                     /locus_tag="MIW95_000321"
     CDS             complement(12167..12478)
                     /gene="rplU"
                     /locus_tag="MIW95_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBY3149681.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12579..14000)
                     /locus_tag="MIW95_000322"
     CDS             complement(12579..14000)
                     /locus_tag="MIW95_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBY3149682.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLIIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(14018..14719)
                     /locus_tag="MIW95_000323"
     CDS             complement(14018..14719)
                     /locus_tag="MIW95_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBY3149683.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14721..16565)
                     /locus_tag="MIW95_000324"
     CDS             complement(14721..16565)
                     /locus_tag="MIW95_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBY3149684.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFI
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            16884..18077
                     /locus_tag="MIW95_000325"
     CDS             16884..18077
                     /locus_tag="MIW95_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBY3149685.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(18289..19371)
                     /locus_tag="MIW95_000326"
     CDS             complement(18289..19371)
                     /locus_tag="MIW95_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBY3149686.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGESVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQEEAILDEVYGNNLRPKIERYEGVPCSLINSSINTIKFTSKNSNLDPS
                     RHTVSVIGFMNTPYGMVALAPDGVPVEYPFEYDKPFSLDFTGKNLSAAELSKKCKNNN
                     VIIKIEIGEIEPNSGRGQVRYARYVN"
     gene            complement(19935..20726)
                     /locus_tag="MIW95_000327"
     CDS             complement(19935..20726)
                     /locus_tag="MIW95_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149687.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTNIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(20730..21059)
                     /locus_tag="MIW95_000328"
     CDS             complement(20730..21059)
                     /locus_tag="MIW95_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3149688.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(21544..21957)
                     /locus_tag="MIW95_000329"
     CDS             complement(21544..21957)
                     /locus_tag="MIW95_000329"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBY3149689.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(21998..23128)
                     /gene="rodA"
                     /locus_tag="MIW95_000330"
     CDS             complement(21998..23128)
                     /gene="rodA"
                     /locus_tag="MIW95_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBY3149690.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(23213..23497)
                     /gene="minE"
                     /locus_tag="MIW95_000331"
     CDS             complement(23213..23497)
                     /gene="minE"
                     /locus_tag="MIW95_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBY3149691.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(23515..24312)
                     /gene="minD"
                     /locus_tag="MIW95_000332"
     CDS             complement(23515..24312)
                     /gene="minD"
                     /locus_tag="MIW95_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBY3149692.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(24336..25019)
                     /locus_tag="MIW95_000333"
     CDS             complement(24336..25019)
                     /locus_tag="MIW95_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBY3149693.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYENESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(25117..28095)
                     /locus_tag="MIW95_000334"
     CDS             complement(25117..28095)
                     /locus_tag="MIW95_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBY3149694.1"
                     /translation="MEENKQVDRLKIIKIVMSVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(28107..28595)
                     /gene="mreD"
                     /locus_tag="MIW95_000335"
     CDS             complement(28107..28595)
                     /gene="mreD"
                     /locus_tag="MIW95_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBY3149695.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(28610..29503)
                     /gene="mreC"
                     /locus_tag="MIW95_000336"
     CDS             complement(28610..29503)
                     /gene="mreC"
                     /locus_tag="MIW95_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBY3149696.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKDMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(29513..30523)
                     /locus_tag="MIW95_000337"
     CDS             complement(29513..30523)
                     /locus_tag="MIW95_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBY3149697.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(30644..31303)
                     /gene="radC"
                     /locus_tag="MIW95_000338"
     CDS             complement(30644..31303)
                     /gene="radC"
                     /locus_tag="MIW95_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBY3149698.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(31348..31932)
                     /gene="maf"
                     /locus_tag="MIW95_000339"
     CDS             complement(31348..31932)
                     /gene="maf"
                     /locus_tag="MIW95_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBY3149699.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(32152..33123)
                     /locus_tag="MIW95_000340"
     CDS             complement(32152..33123)
                     /locus_tag="MIW95_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBY3149700.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(33139..33714)
                     /gene="rsxA"
                     /locus_tag="MIW95_000341"
     CDS             complement(33139..33714)
                     /gene="rsxA"
                     /locus_tag="MIW95_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBY3149701.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(33729..34322)
                     /locus_tag="MIW95_000342"
     CDS             complement(33729..34322)
                     /locus_tag="MIW95_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBY3149702.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(34335..34904)
                     /locus_tag="MIW95_000343"
     CDS             complement(34335..34904)
                     /locus_tag="MIW95_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBY3149703.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQENNEL
                     RKAVLPEAKEFKEMDKSTYKDLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(34904..35881)
                     /locus_tag="MIW95_000344"
     CDS             complement(34904..35881)
                     /locus_tag="MIW95_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBY3149704.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(35904..37241)
                     /gene="rsxC"
                     /locus_tag="MIW95_000345"
     CDS             complement(35904..37241)
                     /gene="rsxC"
                     /locus_tag="MIW95_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBY3149705.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(37457..38401)
                     /locus_tag="MIW95_000346"
     CDS             complement(37457..38401)
                     /locus_tag="MIW95_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149706.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(38512..39786)
                     /locus_tag="MIW95_000347"
     CDS             complement(38512..39786)
                     /locus_tag="MIW95_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBY3149707.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSNQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKSKNNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(39918..40289)
                     /locus_tag="MIW95_000348"
     CDS             complement(39918..40289)
                     /locus_tag="MIW95_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY3149708.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(40517..41116)
                     /locus_tag="MIW95_000349"
     CDS             complement(40517..41116)
                     /locus_tag="MIW95_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149709.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(41317..41529)
                     /locus_tag="MIW95_000350"
     CDS             complement(41317..41529)
                     /locus_tag="MIW95_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBY3149710.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDISIEKLKEVIAELGFELKGIE"
     gene            complement(41602..43317)
                     /locus_tag="MIW95_000351"
     CDS             complement(41602..43317)
                     /locus_tag="MIW95_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY3149711.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEEARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANINSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEKKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(43317..43871)
                     /locus_tag="MIW95_000352"
     CDS             complement(43317..43871)
                     /locus_tag="MIW95_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBY3149712.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(43864..44466)
                     /locus_tag="MIW95_000353"
     CDS             complement(43864..44466)
                     /locus_tag="MIW95_000353"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBY3149713.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNISIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNSLTHPRIREKIISEIEELR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSKQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(44475..47123)
                     /gene="polA"
                     /locus_tag="MIW95_000354"
     CDS             complement(44475..47123)
                     /gene="polA"
                     /locus_tag="MIW95_000354"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBY3149714.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAENNGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPAQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMVFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDTEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETIPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            47442..47524
                     /locus_tag="MIW95_000355"
     tRNA            47442..47524
                     /locus_tag="MIW95_000355"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47476..47478,aa:Leu,seq:caa)
BASE COUNT        14748 a         8991 c         5306 g        18935 t
ORIGIN      
        1 aactatattt ataaaaatca ttttattaac tctttattat ataagataca atagtcttac
       61 tttatttatg tacacatgct tttaggatga caacatcaac ttaaatatta tgttactttc
      121 taaatttaag taaatattgc catcctaaaa attttacaat ttagcttcag atttaaaagt
      181 aaagtttaaa atattatatt taaatctaat ttaatatttt ttgttttgta aatattatta
      241 aattaatatt tctaatatat tttccctgga tttaaaatat ttttagggtc aaaagctaac
      301 tttatatttt taattaacat catataagca tctgaatttg attcatgtaa gtactctttt
      361 ttagcatatc ctatgccatg ttcaccagaa acttgaccac taagttctct agatttttta
      421 tacatatagt caaaagtttc ttttaatctt attttccatg tattatcatc cataccatct
      481 tttaaaatat atatgtgtag atttccatct cctgcatgac caaaactttt tattcttatt
      541 tttaatttgt cttgaagctc gtgagtgtat cttataaact ctgctatctt atctcttgga
      601 acaacgacat cacattcatc catttgtgtc gtagatgcct ttatagcttc taaaaatgca
      661 cctctggctg accatataga atcgtttctt tcttgtgtat ctgatataaa tacatctaat
      721 gcaccatttt ctaaacataa attagctact ttttcatatt ccttttctat atcttcagtg
      781 ctatttccat caaatgtaag taaaagataa gcatctgatg tattatctgg aaacttttta
      841 cctaaaaatt cttctgctgc caatattaca tctctttcca taaattctat agccgttggt
      901 attgattttg attttattat tttaggaaca gtttctattg ccattgataa atctggaaat
      961 ggtataagca aacttatact tttctttggt aaaggaagca attttaatat agctttagta
     1021 actattccta aagtaccttc tgaacctact aataaatcct ttatactata tccagaacta
     1081 tttttaacca cctttccacc tacatttata atttctccat ttggtagtac aacttcaagt
     1141 cctcttacat aatcccttgt aacgccatat tttactgctc tcataccacc tgcattagtg
     1201 tttatattac ctgcaattgt agcacttttt tcacctgggt caggtgggta aaataaatct
     1261 ctatcttgaa catattgacc tattgtcatc aataaaacac ctggttctac tgtcaatgtt
     1321 aaattttcat aatcaacttc aagtatttta ttcattttac atagattaat cattattcca
     1381 ccatttatgg caacagcagc acctacaaga ccagtacctt gacctcttgg tgtgactggt
     1441 atattatttt tatatgcgta tttcattatt ttagatactt gttcagtttc aagtacattt
     1501 actaaaactt cagggtactt ctcaatccct ccaagttcat catgtgaaaa atcttcattt
     1561 atatcacttc caactaatac attttcttct ccacaagtat ctattaaaaa atctatatct
     1621 tttttatcga ttaatttata catttctatc cccctattta gctgctgatg ccatatattt
     1681 ttgattatta tctttgttat tttctatttt ttcaatcaat ttaggaataa tatcataaat
     1741 atcacctatt aaagctaaat gtgctacatc aagtataggc gctttatcat ctttatttat
     1801 tgctactata tagtcagcac cctgcatccc tgcaacaaat tgaactgctc cagatacacc
     1861 acaagttatt atcaatttag gttttacagt tctaccacta agacctattt gttttttagc
     1921 atctatccaa cctgcttcta ttacaggtct tgttcctgcc acttgagcgt ttagcttatc
     1981 agctaattta tatatcatct ccatatcttc ttgttttttt attgctctgc ttgccacaac
     2041 tatcacttca gcttcttcca gaccaacttc tttatttttt tcttttacac tcagtacctt
     2101 aattttagat tttagttctt ttttttctac tttacataaa gttatctttc ctgtagtatt
     2161 ctctattttt tctggtgctg aaaatatttt atatcttaca gtggcaaatt gtggtctatt
     2221 attaggtgtg tttatatgag ccattatatt tccaccaaat gctggtctta tttggtctaa
     2281 atcagtattt gattgaatat caagtattgt acaatctgct gtaagacctg ttctaaatct
     2341 agcggcaagt cttggagcta atgacctacc taatgtagtt ccaccaacta atactatcgt
     2401 tggttttatt ttatctataa agttttctat tgcttttgaa tatggttcta tcctaaaatc
     2461 tttaaattca tcttcatcat atacaaatac ttcatccacc ccataagaaa tcaaattact
     2521 gcacttatct tttatgtctt ttcctacaaa tacacaatac acaggttgtt ttatttttga
     2581 tgccatctca cgagctttac ctataagctc gtacgttaca ggatgtatat ttccattatg
     2641 atgttcaaca tatactgcaa ttcccctcca ttcatcttta ttaatttgta ctttactact
     2701 ctctataaat tcaaatgcgc cttttggtcc acttttgaca cacaacttgc acatcttaca
     2761 gccagcactt atttctacta tacctgactt ttcttctata gccccaaaag gacatatttc
     2821 taaaattctc tttaagtcag ttattttatc ttgatttaca actatctttg acatttatta
     2881 ctccccctta caccaacttt aattcctcta atttatttac aagtgcaaaa cttagttcat
     2941 ctgcatttcc tcttacaata tttttatctg tatttggctt tggaggaaat attcttacaa
     3001 cttgagtagg agaaccgtta agtccatagt tattttcatc tttatcttca aagtcatcta
     3061 agctatatac aggtatttct ctatctttag tattaagttt taatttaaat gaaggtagtc
     3121 ttggttgaaa aatgtctttt tctacagtta ttaaacatgg atattcaatc tcacatactt
     3181 gtaaatcatt tggcatatct atttcaacaa ctatactttc atctttaact tcaattaatt
     3241 ttgtaacgtt agtaacatga ggtatatcta aaaattcagc tacttcaggt cccacctgtg
     3301 ctgtatcacc atcagtagtt tgttttccac aaattattag gtctaccttt cccatcttct
     3361 taataccttg tgatatggta taggaagtag ccaatacatc tgctcctcca aatctcctat
     3421 cacttattaa tgctccttca tcagctccca tcataaaact ttcttcaatt accttctttg
     3481 cctgaggagg acccatgcta agtactttta gattcgcttt cttatcttcc tttattctaa
     3541 gagctgtttc taaagcatat aggtcatagg gattcatttt tgaatctact ccatctcttt
     3601 ttagtactcc tgtagtttca tcaacttcta ctgaagtcgt tccaggaact tgtttaatac
     3661 atgtcaaaat ttccataaag tacactcctt ttttatttaa ctataactga tactccatca
     3721 ggtataacta ctactttgct ctccttacca actatactat tcgccatttc taaagcttct
     3781 ttaaagttaa atgcatgttt catatgcatt ttttttatca tttcaggatt acacttatct
     3841 gttaccatta tcacagtata tttagataat attctcgcta gtatctgaaa ttcccattgg
     3901 tctggaatcg tatctactct atcgacactt ctaattttat ctaaaagttg ttttggagaa
     3961 tctgcatttg ctacattttc ataaaaagat tgacctccat gaccatcatt acatgctgaa
     4021 atcattatta ttactccacc gtctttacaa gtagcttcag ctgctgtcat tcctttaaca
     4081 gattgataaa tattttggtc tagtggatat ccaccatttg tagttacaac aatatcagat
     4141 tttatagaat ttacttttga taactctgtt acaaatctac atcctttagt atgtgctttt
     4201 tcgacatcac cagcaaagga agcaattatt tttttatctt tatctattac aacattcaat
     4261 atgaatgcta actttgcaac tttagctgca tataccatat cctcatgaat aggattatta
     4321 ttcaatttcc cagttcttga attatcacta tctataaagt cactacagtg attgtacatt
     4381 atagtctttg cagatgcgat tcctggaagt acgctttttc tgccacctga aaaacctgca
     4441 aagaaatgag gttctataaa accttctgct atcaaaagtt ctgcctcata tgctagtttg
     4501 tttatatata aatctccacc tgaaggcaat atccctactt tacacataga tttatcatca
     4561 gtagatatat gcataactat atcttcatta tctactattt cttctcccat tttataaatt
     4621 agctcttccc ttgtacttgg cctatgaaat ccagttgcca ctataatctt cacatcaatt
     4681 tctggattaa cattcctaat ccttcttaat ataattggca tagttattct acttggtaca
     4741 ggtcttgtat ggtcacttgt aattatgact acattctttt tccccttaac cagttcttcc
     4801 agagaattag agcctatagg attattcata gattcttcaa ctatctcttc ttgagtctta
     4861 tgttctatat aattatccga ttttgactcc aaaacaccga gaaaacgttc atcaggtatt
     4921 tctaaaatca ttccttgttt tgaatacggt acttttaact ttaccatttt taatctccca
     4981 ttataataaa ctataaaatt ggtatgtggt cataccagtt tcacatttat atgatagtac
     5041 cattatctac catttgcaag aataatttat tttataacaa acttttaatt ttcatttttt
     5101 ctataatttt tacttataaa atccatatgt tcctttgctt tctcacatgc aagttctaca
     5161 tcattttcta caactgcatt gtatatagct tcatgctgtt taagcaatat attttcatta
     5221 aaaggttctg cgattatttt ttctcttgca tcttctataa atccatcaaa taattttgat
     5281 gctgtcataa aaagactttg aattaaataa tttttactta atgttgctag tttactgtga
     5341 aatttttggt ctaataacac cagtactcga ttattatctt ttttattagt ttctactctc
     5401 atttcatcta ttatatgttt caattctata acttcagaag aagttattct ctttgaagca
     5461 agttttgcaa tctcaatttc taggattatt ctaagctcta atatatcttc aagtgtgcca
     5521 ttattaagtt taaaaatcat agataaaggt tctattaaag ttttttctat atttgtacat
     5581 ataaaatttc cttcaccctg tctgctttct atcactccca tagtctctaa taccctaata
     5641 gcttccctta tggaagttct actcacattc atttgttcag acaattctct ttctgatagt
     5701 aatttatccc ccttttttaa ctctccattt agtattttat attgtatttg ctcaattact
     5761 tgttcataaa cttttttact agctatacta ttaaacattt tttcctccaa tatgataatc
     5821 gttgttgtaa tatatatcta ttatatatct taaaccatta cattcatata tttttattac
     5881 ttttaatata atatcttata actcaaattt acaggtgata ttatgaaaaa aaataaaatt
     5941 attgcttttt ttgctccagc tattatagtt ggagttctag ttttagggat tatcctatac
     6001 ccaaatgaag ctataaaatc tgcaagagaa ggcttctcta tatggtctaa tgttttagtt
     6061 ccatcactac taccatttat aattggagca aacttaattg ttgatttaaa aatagtggat
     6121 attatagggt ttatcatcaa tcccataact agatttgtat ttaatgtttc aggaaagagc
     6181 gctttagctt ttgctatatc tacagtatct ggttatccag taggagtaag tttagcttct
     6241 gaatttagga gtaatgggca aatttctaag catgaagcac aaagacttgt gtctttttgt
     6301 tctacatcag gacctttatt cataataggt tcggttggta caggtatgtt ccaaaattca
     6361 aacttaggtt atttaatgat tctctgccat tatctaggaa ctatattggt tggtcttttc
     6421 tttagaaatt atggtaatga aaatcttcca aagacaaaat tggatttaaa aacaaatata
     6481 aaaaatgtaa tcaacattag aaattcaagt gggaatggtt tttttgtttt atttggaaat
     6541 gctgtattta atggagtaaa tacattactt acagttggtg gatttgtaat tgttttttca
     6601 gtagtattta aaatactatc tctttttaat gtaatctcat taatagcatc agttatatat
     6661 ttgcctcttt catttttagg tgttagtaaa gaactctgtc aagcatttgt aagtggtcta
     6721 tttgaaatta ctataggatg caataaagta tctagtataa caagttctcc agaaatttta
     6781 agagcatctt tagctagctt tttgatagga tttagtggat tatccatcct agctcaatgt
     6841 tgtacatttc tatcaaaaac ggacatagat ttaaaattat acatactaag taaatttgcg
     6901 catggagttt tagctgctat ctttatattt atactttatc caattgcaaa ctctgctgtg
     6961 ttggtttcta gctttgctga tacatacaac gttatatata ataatttaat ctggttttat
     7021 tatcttagct attatgatac aattcttcaa attgtagtaa taatttatct aatctcagca
     7081 gtatttattg caaaaaaaca tatgaaaact ttatctacaa atgggaaact aatgcgatat
     7141 aaaaatactt tttttattaa gaaattttca cgaaaaaaat aaaactaata aaacttaact
     7201 aaatacttcc atttttttat tatatttgtc ataattttaa taaccttttt aattagttta
     7261 acagcatagt tatttcatca caatttaata attttattag aaatattttt caaaaaattc
     7321 tccttaatca catatatcac acttgtataa gtttactggg aaatttttcc attatataat
     7381 gtttcattta tattatataa taaataccga cctaaactta ccaaatcaaa ctaagaggag
     7441 tggttgaaaa tggcaacaag acctatagaa atagacgaat ataaaaagat aatggaacta
     7501 ttacatactg gctttactta tagtgaaaat ggcgttgaaa aaagatttag aaaaaatcct
     7561 aaagttgcat tggcactaat gttagaagct aatttaggtc taagaatatc agatatttta
     7621 agattaaaga taggtaattt taaaggtaat atgttggaaa taaatgaaaa aaagacgggc
     7681 aaattacagt atagaccagt taataaaaat ataattgagt ccataaatga acatgcaaga
     7741 aagtataatc ttaaatcaaa tgactactta gtaaatataa agacaaaggc tattcaaaaa
     7801 cagttgagga taatttgtaa acacttaaat ctgtataata taagtacaca tagctttaga
     7861 aagctctatg ctactactca atttgaaaag agtaacaata atttagagtt ggttaaagaa
     7921 cttttaaatc acagttctgt agccactaca cagcgatata taagagtaac tcagcaagct
     7981 attaatgaag ctagtgaaaa tttctttatt ggttaattat aacgtataca tttttctatt
     8041 atcttattaa ttcaccagat attaaaaaag agtgtctcaa aatgaacttt ttagttcatg
     8101 agacactctt tttttataaa atcaaatata tttttattat ttcaatactg gaaaaaaggc
     8161 ttgtctctct attttgagac accctattaa attttcataa ttcttgcata aacatattaa
     8221 tctatcttca ataatttttt agctgattca ctgtaattat ttctttttac aatttccaaa
     8281 actctatcaa tctcatgttc tgactttgca cttacaaaaa tcgccaattc ttcatcatct
     8341 ataatatcat aactatctac atatttatct aaacattcta aaattccatc tttattatta
     8401 tacccaccaa atctaactac tgcactacac tcatcttcta taagtccact taaatccaat
     8461 ttcccttttg aaaaagactt tatatttgaa cttctattat ctattatatc tataatatct
     8521 gcataaacag cacttcctgt tgcacctctt ccagctccct tacctacaaa agaaagttca
     8581 cctactgaat cacctactaa aataactacg ttgaactcat tatctatctt tgaaagcata
     8641 ttattattgt ccactaatgc tggtctaaca aatccactta cttctcctga ttcatactta
     8701 cttctagcaa ccaattttat cttacaattt aatttttttg catattctat atctttttca
     8761 tctattgaag ttattccttc taataataaa tctttccaat atactatttt tccataagat
     8821 aaagtagaca atatagataa ctttctagca ctatcatatc ctaaaacatc tgcatctgga
     8881 ttaacttctg caaatcccaa ctcttgtgcc tgttttaaag caacatcata tggtaaattt
     8941 tcatcataca tctttgataa tataaaatta caagtcccat ttaaaatagc gtttatgctc
     9001 tttatattgt tgccttccag tgattctatc aatggtttta aaactggaat accacctgcc
     9061 acagatgctt caaacttgat gctaacatta tttaagttag ctaattctat aaggtcacta
     9121 cctttttcag ccagtaaatc tttattagct gtcactacat gtattttttt atttaacgct
     9181 cttgttacat aatcaaaagc tggatttaat cctccaatta cttcaactag tatatcaata
     9241 tccttgtcaa atatatcttc tatatcagta gttatcttat caaaatactt cttatacttg
     9301 tgtttttctt tatttttaac taaaattcca acaacttcta tatctctttt tgaagtatta
     9361 ttttctatta aatcaattaa tccacttcct actgttccat accctaatat tgctatcttc
     9421 actgtaacat acccccatca ttttgattat attaaattat ttatatatat aaatatacca
     9481 ataaaaaaag cttgagtata aactcaagcc atatgacggg tttatactca tctttcagca
     9541 taaatgctgc aggatttagc accaatgtat aattaaatat actggttgcc gggcttcaca
     9601 gggccagtcc ctctgccact cttgataaga ttgctaatta aaatttatgt ttaatagtat
     9661 aatacaattt cttccaattg tcactatttt tttaacttat ttccctaaaa ggatataaac
     9721 tattatctaa cagctctttt agttggttta cccttttttg taacattatt atttttagtc
     9781 tttgctttcg cctgttcatg tcctggaaaa agtattttag gctcttcaat attcctcttg
     9841 taaatagtaa atttaaaacc aatagcctgc acaaattcag ctcttaaagc ttcacatact
     9901 gcattagcag tttctttagt atcaagacct gcattttcta atatagtaac ttttactaat
     9961 tctctagttc ttaacatacc atctaattgc tctaagaaac tctctgtaac accttcttta
    10021 ccaatttgtg tagtaggctt taaagtattt gctaaagatc ttaaatacgc cctttgtttt
    10081 ccttttaaca tattctcacc tctataattc tatacttaaa ttatctataa tctatattta
    10141 aattaattgt aaaactcaaa ttctaattca taaattttaa ccacatctcc atcttctatt
    10201 cccatttctc taagcctatc aaaaacacct tgactctcca tagctttttg gaaatattga
    10261 agggattcca tatcatcaaa atttacagaa tacattattc ttctaagtgc tttacctgta
    10321 actacataca caccatcttc aatatctata cttaactctt cttcagtacc aatatcaagt
    10381 tctggtctat acatttcttc ttctgaaact agctccacat cttctacttc ttttagcatt
    10441 ttagaaacat aggctattac ttcatctatg ccttgacgag ttgctgctga cattttaaat
    10501 actgtatatc ctctgccttc taattcactc ttaaacttct caaacttact ctcatcttct
    10561 aatatatcaa atttatttgc aactacaact tgtggcctct ttgaaagctt ttcattgtac
    10621 aatttaagct catcatttat cttgtcaaaa tcttcaatag ggtctcttcc ctctaatcca
    10681 gatatatcta caatatgtat caataccttt gttctctcaa catgtcttaa gaactcatga
    10741 cctaatccta tgccttcaga agcaccctct ataattccag gtatatctgc aagtacaaaa
    10801 ctatctccaa acttagtttg aacaacacct agatttggtg taagtgttgt aaaatgatag
    10861 tttgcaattt tgggtttagc tttagttaca actgatagaa atgttgattt acctacattt
    10921 ggaaatccta aaagaccaac atctgctatc attttaagtt caagagttat ccatctttct
    10981 tctccatctg taccagattt tgcaaaagct ggagcttgtc ttactgcatt tgcaaagtgt
    11041 tggttacctt taccacctat accacctttt gcaactatag ctgtatctcc ttcttttttt
    11101 aaatctgcta atacaagacc tgtagcctca tctcttatta ctgtacctgc tggtactttt
    11161 aatactaaat tttctccatt tttaccagct ctttttcctt tgcttccgtc gccaccattt
    11221 tgagcttgat attttttctg gtacttaaag tccattagag ttctaagccc caaatcaact
    11281 tcaaatatga tgcttgctcc acgaccacca tctccaccgt ctggaccacc agctggaacg
    11341 tatttttctc ttctaaatgc taccgaacca tttccaccat ttccagcctt aacaaatatt
    11401 cttgccttat ctataaacaa attcttcacc tacttaatta aaaactttta attagcatta
    11461 ccaaaaatac cattataaat tcatcagtat tataagaaaa aggagtgcct caacactcct
    11521 tttatgtata ttactcagct attgctactg gatatatact aacttttttt ctcttcttgt
    11581 cttttctttc gaatttaaca gtaccatcaa ctaatgcaaa taaagtatca tcactacctt
    11641 ttccaacatt agttcctgga tgtattttag ttcctctttg tctaactatt atacttccag
    11701 cagttactaa ttgaccatcg aatctcttaa cacctaatct tttagatata gaatctctac
    11761 catttttaga agaacctacc ccttttttag atgctaataa ttgtaaattc atgtttaaca
    11821 tatggagtca ccccctactt ttttaaaatt tttatatttt ttgggtattc ccgtttaatt
    11881 tctttaaccg ctaattgaaa gtgatttagt aataattgtg cgtcttttac atgcgtatct
    11941 ttgactatac actctatatg accttcttct cctactactt tttcaaattc aatcttttgt
    12001 aatttgtcta aagaatttat aactgcaata gcattagatg taactgcagc acatactata
    12061 tcatagccta tttcagcaaa atcagcatgt ccctttaaac aaaaaccttg caacaagaaa
    12121 tcatcattat aataatattt aacttttatc ataacttaac caataactaa gcgtttattt
    12181 tttctacaac tatcttagtg tatgattgac gatgtccttg ttttcttctg taatcttttt
    12241 ttgctttata cttgaaaact ataactttct tagctttacc ttgttcaaca actttagctt
    12301 gaacagatgc tccttctact actggagaac ctaattttaa ttctccatct ttagagcaag
    12361 ctaatacttc gtttaaagta acaacatcac ctgcgttagc ttctaatttt tcaacgaata
    12421 atacatcacc ttcagaaact ttatattgct ttccaccagt ctttactata gcgtacatta
    12481 aatacacctc ctcggcctca aactcgccag tacaaggtac ttcatttgaa gatttaaacc
    12541 tttactgtgc ggcattacaa ttattaatta taaaatattt acattttatt gtcaactatt
    12601 ttatcgaaaa taacattggt tttattgtgc tttaatgtac tatttgcgtt taaagaaatc
    12661 tttatattat atttttcacc aattttatca attatatcca tataatattc ttttatttgt
    12721 tcaaaaataa aatcattaaa ttctaccatt atgtttttgt aaacagtatg ttcacttatt
    12781 ctcataattt ctttttcaat actatctagt atataatgta cagattttat tgtttgttca
    12841 ccatcacaac atggacaaga cattaaataa tacttatcaa tggattcttt ttctctacgc
    12901 ctagcaactt ctactaaacc aagttttgtc atacctaata cttcagcttt tcttttatct
    12961 ttattaaatt cttcttctag gatgtgtaaa atctcctttt gatgcttctt tttatgcata
    13021 tcaataaaat cgattattat tatcccagct atgtctctaa ttataagctg tcttacaatt
    13081 tcttttgcag cttcaagatt agttttataa acggtttcat caagtttacc agttccaata
    13141 aacttacccg tatttacatc tataaccgta agagcttctg ttttttcaat tattaaataa
    13201 cctccactct ttaaccatac ttttttattt aataattttt ctattttaga ttctattctg
    13261 tataaatcaa atacatctct attttcctca agacacagtt tatcaacata agtcttatct
    13321 atacctctta aaatagactt aagttcagag tacttatcat aactattggt aattatagat
    13381 tcaatatcat cattcacatt atcttttaca tatttagttg caaaatctaa agacttatat
    13441 aataatttcg gacctatacc aagtttatat tcttttaaaa tattttcata ctttaatttc
    13501 aactcttcta tatctttctt tatttcatca tgagtgcaac cttgagcctc agtccttatt
    13561 attaatccta gattttcctt atttactgcc ttagtaattt tttttaatct aaatcgttct
    13621 ttttcatcag taattttatt tgatattgta gttctatcat tggaaggtat atagacaata
    13681 taccttccag aaatactaat ctcagtgttt aattttgcgc ctttagttcc tatttcttct
    13741 ttatttatct gaaccaaaat atcctgacca gattttatag tacttccctt ttttaattgt
    13801 aaataagcaa gtttcttaaa tccaatatct acaaaacaag cttctattcc tggtataact
    13861 ttttttacga ttccacgata tatattggat acagtcttct tatttaaatt gtcttcaaca
    13921 aaaagttctg ttaatctttc atcttcaagt actgctgttt tctgggaccc tattagggac
    13981 tcaataacta tctttttcac aacttcacct ctcgagctta cataggagtc actaattccc
    14041 catcttctaa tacatataaa tctcttctca atatatcaac atctaatggg tcaatatcaa
    14101 gttcaaacat ctctaatatc tttggtataa gtatattagt atttaaatta gtttttgagc
    14161 ctgtagctat agtagcagta agagtaatat gttcatcttc taaatttaaa acatcaaagg
    14221 ttcttatcat aggtcttata tctacttcaa ccatcttacc ttttttattc ttttttgtta
    14281 tgattatttc tttattattc ataaagtcaa ttacttttct ttttacaaac tctttcgtca
    14341 taggtctttt caaatctata ttaaacaaat attctccata gtctatcaca gaagaaagag
    14401 aaggtgtttg tctatcaatt tcttttgctt tgataaaatc tatcccatct ggaagttgaa
    14461 ttgatacttt atttaaaaat tcctctactg ataaatcatc ttcttctatt tcaatatcta
    14521 catactcacc ttgactttct gtccctaaag ccaaagcatt gccataactc attttagggt
    14581 gaggattaaa tccttgagaa tgtgataagt ttatttcagc tcttctaaac gctctttgta
    14641 acaatctctg aaggtcaaga tgtgatatat atatcatgtc gccttccttt ttaaatttta
    14701 ctcttattat cttactcata ttagcaaagc ccccttccta tatcatgtat atttattcca
    14761 caaccattgc agttatgtct acaatcagaa gtgatttttt cttttttagc attttcgtct
    14821 tctctaatta agaatttttt actaatacct acatctatat ggtcccaagg aaatacctct
    14881 tcatagtttc tatttctatg tgcataaaaa tctatagaaa gattattctt ttccatagct
    14941 tctttccata tatctaaatt aaaatgttct gcccatccat caaatttagc acctagtcta
    15001 aatgcatcat atataacttt tcctattctt ctatcgcctc tagctactac tgcttccatc
    15061 aaactagtct ttgaatcatg ataattgtat tttatattgt tatttcttaa tttatttact
    15121 aaatgtctct gcttattaat aacttcttct gtagtatctt gaccatgcca ttggaaaggt
    15181 gtaaatggct ttggtacaaa tgtagaagta ctcactgtaa cactaaaact tctctttagc
    15241 tttcctccat ttacttttct ataaatatca ataactttat aagctaattt agctatacca
    15301 tctaaatctt cataagtctc agttggtaaa ccaatcataa aatacaactt aacactattc
    15361 caccccatct caaaagctct ttctgttgca ttttccaaat cttcttctgt aacaccttta
    15421 tttataacat ctctaagtct ttgagttcct gcttctggag caaaagtaag tccagatttt
    15481 cttacttgtt gtattttatc agcaatttcc attgaaaaat tatctaatct aagggatgga
    15541 agcgaaatcc caatattatt agatgcatat tcatctacca aataatcagt caactctgat
    15601 aacttactat aatcactcgt actaagtgat gataatgata tttcatcata tccagtattc
    15661 ttaactaatt tatctaatat ttctttaagt cttttaacac tcttttctct tattggcctg
    15721 tatatcatac ctgcttgaca gaatctacat cctctagtac atcctctaaa taattctagt
    15781 actattctat catgaacagt atctatataa ggtactatta gtttttctgg ataatctaca
    15841 gtctcaacat cttttattat ccttttagta ggatttttag taatcccttc tctattagga
    15901 gttacacttt gaactgtacc atcttcattg tacttgacat cataaaaact tggtatgtat
    15961 actccttcta tgctagatat tttatataaa aaatcttctc tagtcgtttt atttttcttc
    16021 cattctttat actcatttac tacttctaaa ttaacctctt caccttcacc taatattact
    16081 acatctataa aatctgctaa tggttcggaa ttataagcac aaggaccacc aaccattata
    16141 aatgggtctt ccaaagttct ttcttctttt aatattggta tattggctaa atctaatata
    16201 tttaagatat tagtgtatga aagttcatat tgaagtgtaa aagtgacaaa atcaaagttt
    16261 gttataggct ctcttgactc taatgcaaat aatggtatac ttttttctct cattatattt
    16321 tccatatcaa ctgcaggaga atacactctc tcacaaaata catcttcatc cttatttatt
    16381 acatcatata atatatgact ccctaagtga ctcataccaa cttcatataa atctggaaag
    16441 caatgtgcat atcttatcaa ttcactatta ctagtatctt tatgaattga atttatttca
    16501 tttccaagat atctagcagg cttttctact ttttttaaga tttttttcaa gtctacttta
    16561 ttcatttatt aaaattcctc caaacaagct ctttattttt attcttattt ataagtagta
    16621 ttatacacga taaaatattt tctatcaaaa atataactaa aatattgaat ttaaaataca
    16681 tattattcac aaaatagaat agctaaaaat atttaagaat attttctatt aaatttttat
    16741 tgaaatacaa tttttttaca aagtatgata tattataaac ataaaactat tgttttacat
    16801 gacttatcat gacatctttt gtttcattca cctcaaaatt gggtatgtta cttatataat
    16861 gataaatttt aggagggtta aaaatgagta aagtttttga agtaaaaaaa gatatctact
    16921 ttactggcgt agtagatgaa ggtttaaaag tattcgacat aataatggaa actgagtttg
    16981 ggacaactta caactcttat ttaatcaagg acgaaaaaac ggttttattt gacactgtaa
    17041 aggcaaattt taaagatgag tttttaagta atttatcaga ggttactgat atagctaaaa
    17101 ttgactatgt tgtaattcat cacacagaac cagaccatgc aggtagttta aaatacttat
    17161 tagatataaa tcctaatata gaagtttatt gtactaaagc tgctaaacta tacttagatg
    17221 gtcaaataaa tagacctttt aactgtcatg taataaaaga tggtgaaatt ttaaatatcg
    17281 gtaaaagaaa tcttagattt ataactgctc cattcttaca ttgggttgat acaatgttta
    17341 cttacattga ggaggacaag actcttttaa cttgtgatgc atttggttgt cactttgcaa
    17401 gtgttgatgc agaggtagtg aatagtgaag attatctaaa atcagctaaa cactactatg
    17461 attgtatagt taagccattt gctaaacatg ttttaagtgc tgtagataag gtggtaggac
    17521 taaacataga atttgatact atattgactt ctcatggtcc aatgcttact aaagacccaa
    17581 tggctgctgt taaaagatat gttgagtggt ctacagaagc tgttaataca actaatcaaa
    17641 atcaagtttc tatattctac ttatcagctt atagtaatac tttagagatg gctaaaaaga
    17701 taaaagaagg acttgataaa gaaggtgcaa aggctgaatt atatgattta gaagatatga
    17761 cattaacaga aatgcacgat acattagtag tgtcaaaagt aatattacta ggttctccaa
    17821 ctataaacaa aactatggtt aaacctatgt gggatttatt ctctgttata gacccaatgg
    17881 ctaaccaagg caagatagca ggagtatttg gttcatttgg ttggagtggt gaaggtataa
    17941 ctatggctga gactttactt aaatctatgt cattcaaaat gcctgtagaa tctcttaaga
    18001 aaaaattctt ccctagtgaa gagacattaa aagagtgtat ggcatttggt gcagaatttg
    18061 caaaattagt taaatagcca ttaatcacac ataatattat ttaaaataaa tgattttata
    18121 tttaaaaata aattaaaatg gagttgtctc aaaattaatt tttctaatct aattttaagt
    18181 tcaactcctt ttttatttcc caaaatgact aatataatga taaatgcacc ctcaccaaga
    18241 gggtacatta tttataatct tttctatttg tatacttgta tatttatatt aattcacata
    18301 tctagcatat cttacttgcc ctcttccaga attaggttct atttcaccaa tttctatttt
    18361 aataattaca ttattgtttt tacacttttt tgataattct gctgcagaaa ggtttttacc
    18421 agtaaaatct aaagaaaatg gcttatcata ttcaaaagga tactcaacag gtacaccatc
    18481 tggagctaat gcaaccatac catatggtgt attcataaac cctattacag aaactgtatg
    18541 ccttgatggg tctaaatttg aatttttgct tgtaaactta attgtattta ttgatgagtt
    18601 gattaaacta cacggaactc cctcatacct ttcaattttg ggacgtagat tatttccata
    18661 aacttcatca agtattgctt cttcttgatc tgctatcata gtgtgattcc caggtccaac
    18721 tggcgcagaa ggcttagctg gcgtaggttt cttaacagtt ggcgttggat tctgtgtctt
    18781 tactgattca ccaacagctg gataggcttt cttgtcaaat ggtaatgggt gagtaagact
    18841 cttgtatatg atgtctgctc ctgctgattt gttggcatca atatctacat gatacaaacg
    18901 tccatcctca aaacgaataa gattccactg atggtcattt gttgtactat gtacataata
    18961 acattcaatt cccatagcat cacataaagc tttcaagctc ctacacattc cagaacaagc
    19021 acctttttta gtaaccaatg tataagcagt attggctccc tctgctgcgt tccagttaac
    19081 atatgttgtg ttgtttttaa tgtgattata agcaacccta atcttagcat ctgtggtcat
    19141 gttctttgta attttcttgt tcataaagtc tgctgttgct tgtgctaccg aattaagttg
    19201 agcttgtgat aactttggac catatatact gttggttcca gctacatagc ttttcggtgt
    19261 ctttgcagta acatctttag ctgtcatacc ataaactgtt acactcatac atgaggttaa
    19321 aaccatcgtt gcaatcaata gtttagtagc taatttcctg atttttttca tattagcatt
    19381 ctcctttata atatatttta ctttcaattc cctaccatta attaagttca ttttagattt
    19441 aaaatatgga ttatcaacat gtgtgcatag caattcacgt ggtcatagaa tatttagatt
    19501 tcttggaaaa tctgttctat atttcctata tagttcgtac acaatccaac tcacaaacct
    19561 gcatttttga tatcatcaca ataaaatgat aataattaat atattcaaaa atgttggtct
    19621 gtaacaggtt tgtcaatttt ctaattattg gtaatgacga gagtgaggag aattgtcaat
    19681 aatacattta aaattaaaat tgtatacact tcatacattc tcccctaaca acattataca
    19741 gattataatt tgtatattca atacattttt tggaaggtat aattatatca aaatatgtga
    19801 ttatgtatag atacaatcca ctatggagaa atataaaaga aaagagtata acaaacaata
    19861 aaaaataaga tgattcaaat tttattttga aacatcttat ttactctaca aattaaaatt
    19921 atttggtgaa ttatttaatt attatttttt attaaagaaa ttttaaatct taatataaaa
    19981 tacgctatta tacataataa tccaaatgca aatgctactg gaattaacat ctccgattca
    20041 aaataaaaga aatattctaa gaaaatatct ggatttacta tattcccatt cttgtctaaa
    20101 aaatcttttc cattatatcc taaatgctgt ataaaattac ttatgttatt ataatctgct
    20161 tccctctcat ataccatatt agcattcaat ccagaagaac ttaaattagt gtttccatct
    20221 ttcatatcta ttacatatag tctaagagct ttgactttac tatacttatc tttatcaagt
    20281 attttttcaa gttctaactt atattcacca atatttgtca tatcatgtct ttctgccaat
    20341 tgctttattt ctccatcaag ttcttttcct aaattataca tattatgttg taaactatta
    20401 ttgtaatacc acataaatcc agaaattaaa aatgcaatta tacttatgta acctaatact
    20461 tttttaaacc ccattacaat tgatttatgc ctatccaatg ataaagatag ttcttttatt
    20521 atattacata attcatactg catttcatca ccatcatcaa atctttcaat tgctttttta
    20581 catgcttctt cttcatctaa tccttctaat ttaaattcat ttgcagattc tataagatgg
    20641 catctcattt cctgttttaa ttctatagta cttttattgt cacatttttt atatagttta
    20701 tctaaatatt catctattag tttcataaat taatccacct ctcccaaaaa ttgattcata
    20761 acctttttaa taaatctcca ttcttgtttc tttatttcta aaaaatcttt accttcatta
    20821 gttaaattat aatattttct tcttccacct gaactttgtt cattgctata ataagattta
    20881 attaaatttt tagattccat tcttttaagt gcaagataca ttgttccttc ttttaattca
    20941 aactcacttc tttctcttac ttgctttgca atttcatatc cataacaatc tttcttttct
    21001 aatatagaaa caattaatat atcgatataa ccttttaaca cttctttatt taactgcatt
    21061 atgtaccacc gccttttcac ctactactct ataatgtata gtacttatat ttactataat
    21121 aatacatagt actctataat acaaggttta tattctacaa tagacttaaa aataataaat
    21181 agctgtatat tagtatttat agattaaata ctaatataca gctatttatc aaattttaaa
    21241 aataaatttt tgttttatta atgttgtcgt aacaacattt acttggttga aatagtatta
    21301 cctataagtt taaatatata taataataga cttaattcta tttcatatta ttaaaataga
    21361 gtaatagttt aaattctact actctatcta taatctattt cctaattatt taatctcaaa
    21421 tttagctcct catcatttaa atctttcata agatttctac aggtactagg tttattgcaa
    21481 cagaaaaact agggcattaa acacttatat atctgtttct ttagactgta atattaatat
    21541 tttttaaata cgttgacttt tgctttttct aacttctctc catgatagtt ctcctctatc
    21601 aagagctttt ataaatattt ctgctgaagc tagatttgta gctattggaa catgataaac
    21661 atcacaaagt cttattaacg cttgtatatc tgtttcatga gcctgagatg tcaatgggtc
    21721 tcttaaaaaa ataactaaat ctatttcttg tgttactatt aaagaaccta tttgttggtc
    21781 tccacctaaa ggaccagatg ctaatctatt tatatttaat tcagtttcat ccattattct
    21841 ttttcctgta gttccagttg catatagtcc atactttttt aaaattgatt cataacctat
    21901 acaaaatccg accatagtgt ttttcatttg gtcatgtgct actaatgcta tattcatata
    21961 tctaatctcc tcaatttatg tttagatgta tttaaattta gaaatttatt ttctttcttc
    22021 tcatacacac atttagtact aatcctaagt ttgctaaaga tgtcaataac gaacttcctc
    22081 cataacttac aaaaggtaat gttactccag ttactggtat taacgcaacc gtcataccta
    22141 tattttggat tatctggtaa ccaaacattc ccataacacc aaccactatg agggttccat
    22201 aaaaatcttt tgcatctctt gcaattgcta aaagccttaa taggaatatc ataaataaga
    22261 ttatcaacac tgccattcct attacaccaa gttcttcacc tacaacagca aatataaaat
    22321 cactatcttg tacaggtaaa aaatcttctt gattttgact tccattgtaa agtcctttac
    22381 cagttacgcc cccagaacca attgcaatca gagattgcat aacttgatag tttcctttta
    22441 gagttacatc ttctggattt agaaaagctt ctattcttac cttttgatgg tctgccatca
    22501 ttaaatacat cagtggcatt gacactaata gtatgattat tcctcttttt atcaactttg
    22561 agctaagacc tgctgtgaac agcattgcaa atatcataca acaaaataca attcctgttc
    22621 ccaagtctgg ttgtgcaatc aataatccga taaatggaag agaataaact acaacaggca
    22681 taacttcctt taacgtattt aatttatcct ttttggattc aagtatcttt gcataactta
    22741 gtacaaatgt taatttgact atttcagatg tctgcaagtc aagtggtcct aagttaatcc
    22801 acgacctagc tccacctttt actgtcccaa ttccaggtaa taaaactatg gctaacaaaa
    22861 taagacttat tatataaagc gctttataat acctgcctag aagattatag tcaaaaaata
    22921 atatgactat aatcattcct ataccaagta caaaagctaa tccttgttta atcaattgat
    22981 tatatgaacc tgttgaatta gcatgtgttg cactactaag tataacaagc ccaaaaataa
    23041 agatagctaa tactgtaact ataagtttcc agtctaactg ttttatcaac ttaattgtcg
    23101 acttataatt aatattgatt tttttcaaag aatcaccgtc tttctatctt taaacatata
    23161 attaaataaa aggggcatat accaataagg atatccccct tttaagcact aattacatac
    23221 atctatcttt tatattcttt agtggtatat tagcaactaa tgcagataca ggtttgtcat
    23281 catgctcacc tctgcattta gacattttta tttcaagacc atcatcttct atttctgcat
    23341 aattagctat tacttttaaa atgtctgttt ttatcatctc taaaagttgt ggtgaacaat
    23401 ctactctatc atgaactaaa actagcttta acctctcttt agcaacagat ttactagttt
    23461 tagcctcgtt agaaaaaact ctaaataaat ctaacacaga tgtatcctcc tttgctactt
    23521 agccatgcta aacatttttt taagtctacc aaagaatcca tcttcaactt caagatcaag
    23581 tagaggaact tcttcattta gtatcctttt tgcgatattt ttgtatgctt gaccagcaag
    23641 tgacttagaa tcaagtatag ctggctctcc tttatttgtt gatataatta tgctttcatc
    23701 atcaggaaca agacctaaca aatctattgc taatatttct attatatctt gtttatccat
    23761 catgtctcca cgcttaacca tatcatttct aattctattt ataactaatc ttatctcttt
    23821 tatttcattt gcttctaata gacctattat tctatcagca tctcttactg ctgatatttc
    23881 tggattagta actacaatag ctctatctgc cccagctact gcatttttaa agccttgctc
    23941 aatacctgct ggacagtcta ttattatata ctcgaaagat tcttttagtt tctcacacag
    24001 atcaatcatc tgctcaactg agacagcatt tttatctcta gtttgtgctg ctggtaataa
    24061 atatagatta tcaaatcttt tgtcttttat tagagcttgc ttaagcctac aagttccttc
    24121 aactacatct actatgtcat aaactattcg attttcaaga cccattacta catctaagtt
    24181 tctaagtcca atatctgcat ccacaactac tgttttttta ttttctaggc ttagcgcagt
    24241 tcctaagttt gctgcggtag tagtttttcc aaccccacct ttaccagatg ttataactat
    24301 aacttcgctc ataatactac cccctatttt tctttctatt tgtctaactt tgacaaatag
    24361 ctttcgatta caattcttcc attacttaca aaggctattt ctgggctaat ttctgattca
    24421 ttttcataat tttcttcatc atctggagct tctgcaatat tatctgctat ttgtaatatt
    24481 ttaggattaa gatttccagc aacaacatat gcaaattcat tgccttctgc acctgcatgt
    24541 acaaaacctt ctattttacc cattacaaaa acattacctc ctgctacaac cttagcacca
    24601 gcatttatat ctcccataac aactgtattt gattttgaaa ggacttttga accaggtttc
    24661 atatcattca atataactac atcaccttca aattcaatat tttcaccaga tcttagggta
    24721 ttgacatatt ttgttggaaa attactattt tcttcaattt tttgatcttc aacaaattcc
    24781 acatcaaatc tagaagtaat tccttcttta agttctagaa tctccatgtc agttaaacaa
    24841 tcactattaa ttttactaat tttagcccca ttaaaaaagc caacatatgc ttccaatttg
    24901 tttattattt tttcctgtat ttcctcaaat ggagcttctc tttttatatt tactattatc
    24961 cctcttttat tacccttgaa ctctactagt tcttgactac aaatttctct taaagacata
    25021 acaacctcca gagtgatata tcatttatat tcttcaaata cgctttaaac ccttttattt
    25081 tttctatata aagctaaagt ttatacatag attaagctat ctattaattt gtgactcgaa
    25141 atttatattt tcgtcagaag aattgttaac atcattcgtc ttgttgtcat ttttactatt
    25201 agttggctct aatcccatat aagttccaat aacttctcta gttggtaaaa gtgagaatac
    25261 acttgaatca ccttgaggaa ttacacatac aacagctatt tctgggtcat ctgctggagc
    25321 aaaagcaaca gtccatgtaa aagaaccata atcttgttta aatctatcta tttggtcatc
    25381 tgttattttt ggatttaatt cttttatagc ttttcttaga tatgaagaat tcaccttatc
    25441 agtgtcctct aatctaactt ttactccatc ttctaattct tcctctaatt tctttctttc
    25501 ttcgtctttc aaatctttat tttctaattt cttttttatt tccttttcct tagctaatga
    25561 aagctctttt tctttttctg ctttcatctt atcagctagt ttaatcgcat catttaggtt
    25621 gacattataa gatgacatat gactttttaa atattcatac tcgttatctg taggtatttt
    25681 cccacttttc tcagctgtac cagtttttgc tgctacatct attggaaaat cagcaaaagc
    25741 actcttcgct gtaccttgtc ttgcaaccaa tttcatacct tttgttaatt cttttaagtt
    25801 atctggattt ttaaaaggta ttttttcgac tttcttctgg tcattaattt taactgatga
    25861 ataatcactt gaaacagctc tatcaactac agataattct actaaattac ctccatttgc
    25921 tatagcagct acatatcttg atatttgagc tggtgtataa gcattttcac cttgacctat
    25981 agctaagtta aatgtatctg cagtactcca ttttgcaaag ttaaaataac tgaatactgc
    26041 taaatctgct acttcttcta ttctatcttc tttaacattc attttcttta gcctattcat
    26101 tgtttcgact ctaccaggag ttttcttttc tgctgcccag cttacaatct cattgattct
    26161 tttctcatat tctttaggat ttttttctct agttatatca acaaaatcat ttgccatttc
    26221 tctttctaaa tttgacttta gaagagcttg tgttgatttc aatttatctt ctgtgctagg
    26281 aactttacct gctctttctt ctacttcatc ttgaagccct gtattttggt caagcccaaa
    26341 taattttgca tactcaagta ttttatttgc attcatatct ataccaatgc tttttccatc
    26401 tggccatgtt ttacctgtac caattgttgc catatatata ttacaagact cttgtatcgc
    26461 tttatataag tttgtcatac catggtttcc tctaccatgg ttccatacat agtcaccaaa
    26521 agatttttta cctaacatta taaccccagg gtcatttata gtaaagtttg gatccaaacc
    26581 attttctaat gctgccattg aagttaccat cttaaatgaa gaacctggct gaaattcccc
    26641 ttgtgtaacc aagtttaaaa gtggacttcc tgctagtaag tcatttggat tttttggttg
    26701 aagtgcttta taatcctctg tagaaatacc ttttgcaaat ttatttgggt cataattagg
    26761 ataacttgat gaagctaata cttctccatt tttaacatca atagctataa gagctgctga
    26821 ctgtgctttt cctgcatatg cactaattgg tttgtcccca aatttacttt taaaagtacc
    26881 tcctttagaa gcaacttcga ttatctgttt taatgcatta tctgaaactt cttgtaaatc
    26941 tttatctagt gtaagataca cagtatctcc tgatttaggt tttttagatt caatttcttt
    27001 actgattcta cccaatgcat caactttaac cattttataa ccatcagttc ctcttaatct
    27061 actttcatta ctcttttcta ctcctgcaag acctaccata tctccagttt cataaccttt
    27121 ttgaaggtat gattctattt gagtggatgg catttttcct acatatccta acatatgaga
    27181 agctaatgac ccattaggat aatatcttac tggttctact gctattgaaa caccaacaag
    27241 gtccatagca ctttcttcaa tttgagcaat cgtagtttct cctacatcct tagcaatagt
    27301 tactggatta tattgagaat atccttttga ttttatcaaa tcccttacga ccattatttt
    27361 tcttgcatct tcatcactca aacttttatc aatttctaat gcatcactat ttcttacttt
    27421 ttcaaacgct tctttggcac tcagtttagt ttcttttatc ttataactag ccaaccaatc
    27481 tctcttatct ctttctgctg tgaacatcca tttactaact aaaataggtg gataatatcc
    27541 attttcattt agttttgcct gtaatcttgt cgcatccaat cttttatctt cttgagataa
    27601 tattccagct gatataagtt tgtctactaa gtaataaaaa ctttccttag cattataatc
    27661 atttggaata ccattttctg atttatattc cctaatatct ctgtcatatg tataatagta
    27721 ctttccattt tctacatata taggaaactc atctacatat tcttcaccat ttctctccag
    27781 caaatttata agttttaatg atatttcatt tgctcttgaa tgcttatttg catctttctt
    27841 attaatatcg ttacctgaaa cttgaacagt aaataagttt ttatttcctg ccaacagtct
    27901 tccatatcta tcttttattt ctcctcttgg agcttctatt gccaactttt tatatgtttt
    27961 attctcagct aactcattgt aatattcata tttaaatgta gtcatataga ttatttttac
    28021 taaaatagct aaaaatatca cgctcatgac tatcttaatt atttttagtc tatcaacttg
    28081 tttattttct tccatgaaat caccaactaa tcttctttaa gttttaaaac tgctctttta
    28141 gataagcgat atattactaa accaattaaa ctattcacta ttggtgctac tagtagacct
    28201 tttacaaaaa gtgttgatat gttgtagctt tgataaatta tactagatgt acctatattg
    28261 acaattgaat caattaaact gcctataaaa attaaaacga aagtaataat ataactacct
    28321 ttatatagct gatctctcat gtatccaatt gcatatgcaa taataaacaa aattaatgca
    28381 tttacaccaa aaattccacc aacggttatg tctttaataa gccctattat tgctgctata
    28441 atcccacctt ctaattcatc taaataaaga gagattatgg ttacatatat aagaactaaa
    28501 ttaaagctta tgccaaaaat atctatataa tttgttatac tattttctat agtcataagc
    28561 acaatcccca acagacaaag taaaactttt ttcattgttt ttatcccctt tatcctatat
    28621 ttcttggttc tatgactaca acgtcattta tattcttaaa atcaacataa ggttttacaa
    28681 ctacatattt taatgactta tctttatcat caaccacctt ttcaacttcg ccaattggta
    28741 taccatctgg gaagaatcca agacctgatg ttgttattat atctccttga attacattat
    28801 atgatgagtc aaacatatat ccttgcaaat atcctttact attataacta tctttattat
    28861 ctaaagtagt attttgagta attgtacctt tagcattggc atcctttgct agtttaaagc
    28921 ttacagatgc cttagaatcc aatagtgaaa ttgccttact ataattgttg gaaacttcat
    28981 aaactatgcc aactaaccca ctaccattca taactatact ttcttttttt acacctgaat
    29041 tttttcctgc acctatgaca aaactctcat accagtttcc atcattttta ccaacaacac
    29101 ttgctgaaat acttgtcgcc ttatactctt cttgtacaaa gttcagtgtc ttttttaact
    29161 cttctagcga ttctgtttta tctagtttgg catttaaagc tatcatatct tttttaagtt
    29221 tttcattttc ttcttccaat ttttttacct ttttagaatt atctttaaaa tttgctacat
    29281 ctttaaaacc atttttgata aaagaaaatc catcatttat tcccttacct acacttgtaa
    29341 tagcatctga agctactcct aaatttactg gactaccact agaaaattta ccaattgata
    29401 tacccacaat tccaattaaa gtgatagcaa ctacgcccgt tgctatcact ttaacattaa
    29461 tccttttttt ctcatttttg ttcttgtcaa atctcaaggc catcaccaac ttctaattct
    29521 tagtattcat cattaatact ttttcaaaga tttcttggtc ttcaacagat ttacctgttc
    29581 caagtgcaac acaatcaaga ggtgtttctg ctatttgtac agacatacca gtttcctgtg
    29641 taataagctt atcaagacct ctaagcaaag cgcctcctcc tgttagcatt attccgtttt
    29701 ccattatatc tgaggctaat tctggtggag ttctttctag tgtagatttt attgcatcta
    29761 ctattgaact tacaggttct tttaatgctt ctctaacttc tgtagagcat atttctattg
    29821 ttttaggtaa tcctgagatt aaatctctac ctcttatttg catatttatt tcttcatcat
    29881 ctttaaatgt agaacctatg ttaattttta cattttcagc agttctttca cctatcataa
    29941 gattatattc tttttttaca taagccacta tagactcgtc aaattcgtct ccacctattc
    30001 ttatagattt agcagtaacg attcctccta aggatattac agctatttct gaagtacctc
    30061 caccaatatc aactaccata ttaccttctg gttcttcaac ttttaatcca gcccctatag
    30121 ccgctgccat tggctcttct ataaggaatg catctttagc tccagcttgt cttgcagcct
    30181 cttctatagc tctcttttct acttctgtaa ctccaaaagg aacacatata gctattcttg
    30241 gacttacaac acctttttta tcagccgctt tttgtataaa atagctaatc attgattgag
    30301 ttatgtcaaa atcagctatt actccatctt tcataggtct tatagctact atatttcctg
    30361 gtgttctacc tatcattttt ttggcttctt cacctactgc taatacctct ttactatcat
    30421 ctcttattgc gacaactgat ggttctctaa caacaatacc ttgtcctttt atgtaaacta
    30481 atgtatttgc agtccctaaa tctattccca tatcctttgt catcttatta aaactaaaaa
    30541 aacctttttt ttctttcttt ttttccttag ccatgatttt actccttcct actattaaga
    30601 atcatgttta aaacattctc ctattatttt atcacatata aagtcaaatt atcatatttt
    30661 ctttaaagct aaagtataat ccatctccaa taattatatg gtctattatt tctattccaa
    30721 ttatttctcc acatttaata agtcttgatg tgatattttt atcttctctt gatggttcta
    30781 cacttcctga ggggtgatta tgcatcacta ttatcttact tgcacttctt cttatagctt
    30841 ctttaaaaac ctctcttgga tggactaatg acgagcttaa agtccctata gatacatcta
    30901 catcagatat tatctcattt tttgtgttta aaagaactgc tttaaaaacc tcttttttta
    30961 aataccttag gctatccata taatacctct gtatatccca aggattcctt attttatact
    31021 ttataggttt aaaactagct actctagagc ctagttctaa agctgcttta atttgggttg
    31081 attttgataa cccaattcca tctatattac acagctcctc tattgtcata tcttctaaat
    31141 gtctaatacc ctgaatatct ctatttatta tatagttggc taattcaata gcatttttat
    31201 tcttgttgcc agttcttaaa agtatagcca acaactcagc atttgataag ctttttacgc
    31261 ctttggcaag catcttttct ctgggtcttt cctctggtgc catttctttt accttaatgg
    31321 ttgaattaaa tgactttttc agataaatca cactccataa aaaagattta tactaaaata
    31381 tttttttaat aggtcgccta accttgaaat tggaagacca actatattaa aataatctcc
    31441 tctaatttct tcaactaaca aagctccata accctgtata ccataggctc ctgctttgtc
    31501 aaatgactca ttagttttca gataatcctc aatatcctgc tctgataacg ttttaaattt
    31561 cacattgctg attacatagt caattacctt tttatcttcg cacaagttta ttaagctaat
    31621 acctgttatg acttgatgct ctcttccaga tagtcttttt aacatatctc ttgcacaaga
    31681 ctcatctttt ggttttccta aaatagcatt gtctaaaaca acaactgtat cagctccaat
    31741 aacaacatct tcattatgtt cagatgctac agacatactt ttttcaaaag ctaatctcat
    31801 aactaaatgt tttggtgttt caccttccaa tataatctca tcaatttcat ttttaattat
    31861 atcaaatctt acattcgtat tctctagaat ttctttcctt cttggagagg ctgatgccaa
    31921 gattatattc atttattcac ctcatttgta cgtaatttgt acctatttag tctatcacta
    31981 tattgtaaaa aattcaacag aaatgtcgaa ccttttttgt tacactattg ttatatttat
    32041 taccattaga ataaatatac ataaaataga ggtatggaaa ttattccata cctctgtttt
    32101 atgtactcat tagagatatg gaaattattc catatcctta ttttgtccat attacaaaat
    32161 tttaatagct ttctttggac atttttccat acataaatgg catcctacac atttatctgc
    32221 atctacctta tgtttttctt ttaattctcc ctctattgca tcaaacttac attgtttttt
    32281 gcacactgta catccaacac aaagttcttg gtcaatagta actttctttt tcttagttat
    32341 gtcacctgat attacttttg tagggcattt tgctacacat tgcatacacc ctacacactt
    32401 attagggtct atcttagcta ttttgtcttc aaatattatt gcatcaaatt tacaagcttt
    32461 tacacacatt ccacagccaa tacatccagc agtacatttt tccttaacag ctttaccaaa
    32521 ctctttgcta ttacactcta caactacttc ttgactttct ggttttgtga ttattatacc
    32581 cttaggacaa acttctttac attttccaca atttacacac ttttcttcat ctattactgc
    32641 tattccatca actatagaaa tagcatcaaa tttacaagca tccttacatg ttccaagacc
    32701 taaacatcca aatttacaca tcttagcacc tgagtttaaa acatttgctg ctctacaatc
    32761 tgatattcct tcatactcat atttatcttt tgcacttgaa caagttcctt tacaaataac
    32821 ttttgctact tgtttctcac ctgcttcagc acttattccc attatttctc caacttttgc
    32881 accaacatct gctccaccta cagggcaacc attaactggt gcattgcctt caactatcgc
    32941 atttgcaaga cctccacaac ctggaaatcc acaacctcca cagttggctc ctggaagtac
    33001 tcctagtata gcttccactc tctcgtctac ttctacagca aactttttag atgcgaaatc
    33061 tagtactatc ccgaagatta agcccataat tcctaaaact aatacagctg taagtatcac
    33121 cattttcacc cctctttttt atagctttat aagtcctgta aatcctaaga aagctattga
    33181 cattaaacct gctgatataa gcgttatagg gaaaccttta aatgcttctg gtatatcagc
    33241 taactctagt ctttctctta tacctgcgaa tattactatt gctaaagtaa atccagctcc
    33301 ggctccaaat ccatttatta ttgtttctac taaattataa cttttttgaa cattaactag
    33361 agctatacca agaactgcac agtttgtagt tataagaggt aaataaactc ctaatgcttg
    33421 atataaagat ggactcatct tttgaattac catttcaacg aactgaacta tagaagctat
    33481 aaccaatata aaagctattg tttgtaagaa tccagttcca gtttttacta ataaaatctg
    33541 tataaaataa gttataattg aagctagagt taaaacgaac gtaaccgcaa ctcccatccc
    33601 tactgctgta tcaaccttct tagaaactcc caagaaagga catataccca agaattgaga
    33661 tgttataacg ttattaacaa gaacgatact taaaaataaa agtattaaat tcatttatta
    33721 cacctccact atgctttttt acttcttaat ttattaaatc ctgcaaataa gaatcctaat
    33781 gttaagaatg cacctggtgg taatataaat attaatactg gttggaaaga tgctccaaat
    33841 aatgtcattc caaataaact accatttcca agtaattctc ttactgcacc tattattgta
    33901 agtgcaacag taaatccaag accttgacct acaccatcaa ctattgaagg catagctcct
    33961 gttttgaaag caaaactttc tgcacgagct aatattaaac agttaacaac tattaatggt
    34021 atatatatac caagagcctt atcaagagca ggaacatatg cttttaaaag catacctact
    34081 attgtaacaa atgtagctat aactactata aatataggta ttcttatctt atctggtgta
    34141 atttttctta ttaaagatat aactaaattt gcacatatta aaactgctgc tgttgaaaga
    34201 cccataccaa ttccatttat tgctgaagtt gtaacggcta gtgttggaca catacctata
    34261 acttgaacaa atgttgggtt ctcatctatt agcccatttt taaatacttt agctaaattc
    34321 ataattaacc ctccttattt attaagagca ctattgaaaa catctatagc agcatttact
    34381 cctgtagtaa ctgctgtaga tgtaatagtt gcacctgata ttgctaatat ttgattatct
    34441 gctgttgtac ttccttttgc tacttcaaga ggctcagcag tttttccttt gtattgaccc
    34501 ttaaatgcat catccttagc ttttgctcca agacctgctg tttctgacat attaccaatg
    34561 tcaacacctg taacttgacc ttcagatgat atacctacca taacttcaat ttctccacca
    34621 tatccacttg gtttagcttt taaagtatat ccaaccactt cagaaccatc taaaccttca
    34681 tacacttcag ctataagacc atctccaagg tctttatatg tacttttatc catttcttta
    34741 aattcttttg cttctggaag gactgctttt ctaagttcat tattttcttg tatatttctt
    34801 tgttctatta ctggagctgt aacctgatta gtagctccaa gagctaatgc agctattgca
    34861 cttatagcaa gtaaagtacc acctaatctc accatactgt tcattattta gccacctccc
    34921 caaatactct atttctaaca aacttatcta taagtggagc aagacagttc actaatagta
    34981 ttgagtaaga tacaccttct ggatatccac catacattct tataacagtt gttataagac
    35041 cagctaatac agcgtatatt atttgacctt ttttagtcat aggcgaagtt gtataatccg
    35101 taagcatgaa gaatccacct aacataagtc ctccagcaaa cagttgatat attgcgaaag
    35161 ttatattaaa tccacctaaa aggaatgtta atataaatac agttgctatg taaaatactg
    35221 gtataacata atttattatg cctttataca ttaggtatac tccacctaat aataataata
    35281 ttgctgatgt ctcacctata catccaccaa tattccctat catcatgtca gcaagactaa
    35341 ttccattgct agaaaggtct gccagtcctg ctgctccatt tttcaaaaag cttaatggag
    35401 ttgctgtagt aactgcatct aaatttttac ctatccagtt aacacctggt tgagtccaag
    35461 cagtcattgc tactgggaat gatgctaata aaaatgctct tgcagcaagt gctggattga
    35521 caaagttaca tccaattcca ccaaatacca ttttaactac taatattgca aatattcctc
    35581 ctactagaca catccaccaa ggaagtgatg ctggtacgtt aaatgctaat aataatcctg
    35641 ttacaacagc tgaaaaatct cctatagtac ttttttgctt cgtaaatttt tgatataaaa
    35701 attcagtacc tactgtacca agtatacaaa aaaacatagc actcaaagca ttaagtctaa
    35761 agaagtatac tcctgctact gctgctggaa ggagtgctat aataacttgt ttcattatat
    35821 atgaagtatc ttcattactt ctcacatgag gagaagatga tactatcaac ttattttcca
    35881 tcgtttttcc cccttaactt tctttatttc tgagcagctt gctttcttct ctttgctcct
    35941 atttctctct ttgctgatct tatagattga agtaatggtc ttcttgctgg acaaataaat
    36001 gaacaagacc cacattctat acaatccatt gctctatgat attcagctgt gtcatagtca
    36061 tcttttaaag aatatgcact tatgaaaagt ggttgtaaga atgatggaca tacatctgta
    36121 catcttccac atcttaaaca gttttgaata tctggagtac gtgattcttc ctcatccaag
    36181 cataaaattc ctgaagaacc tttgttagtc gctatttcag tagtatattg agctattccc
    36241 atcataggtc cacccattat aaccttacca atcttcttat cttctttaaa tccaccacat
    36301 tgttctatta tttctgaaac taatgttcca acttttgtta ttaggttctt tggctctttg
    36361 atgcaactac ctgttattgt agtgattctt tcaaccaaag gcatacctgt tttaatagac
    36421 ttagctattt gtgctgctgt ggcaacattg tcaacaactg cacctgctgc tattggtaat
    36481 ccaccagatg gaacttcttt gcctgtacac gcataaataa gttgtttctc tgctccttgt
    36541 ggatatttca cttgaaggcc aacaacttcg atttcactat agtctttagc tacattttga
    36601 attgcttcta tagcatctgg tttattcgtt tcaattccta taaatccttt tttgacatct
    36661 aataccttca ttaatgctct taaaccaaac acaacatctt ctggattttc aaccattaat
    36721 ctgtggtctg cagttaagta tggttcacat tctgcaccat ttaatataac tacttctgcc
    36781 ttgctatctg gtggaggaga tacttttaca tggttaggga atgtagctcc acccatacct
    36841 actattcctg cctcttttat gatacctact atatcttctt tgctcaaatc ctctaactga
    36901 ccttttggtt gaacactctc atgtaattct tccttaaagt cattttcgat aactacacat
    36961 tgagctgaac ctcctggtac ttcatgttgt tcaatagcaa taactttacc tgatactgaa
    37021 gaatgaacgt tacatgaaac aaaaccttgt tgttcaccta ttttttgccc aaactttact
    37081 tcatcaccaa cttcaacaat aggtttagct ggtgccccta tatgttgctg aagaggaatg
    37141 taaactatct ttggagcttg agctttttca agtgcctttg tgttagagta ctcttttcta
    37201 tatggaggat gtatccctcc cttaaaagtt aagagtttca ttgttttacc cccttatcta
    37261 taaaaaaaca ttttttctgc cttctataag tatactatat atattaaaaa atcttaacaa
    37321 ttgttaaaaa aatatccgta tttttataat ttattttata aatcaatacc tctatattaa
    37381 gtcaaactga ggaaaaggat tgcttaatta aacaatcctt ttcatatagc tttctaaagt
    37441 ccaaattttc tgtattttat tttatagaat ttataaacat agagtactcc tgcatggatg
    37501 ataaaagcag acttttactt acactatatt tttcttcatt tgctgctttt gaagctgtat
    37561 caattttttc attcacattc tttacatact ctattaaatt atctttaaat aaacgcattt
    37621 cagaatagtc aatcttttca gcttcttttg atatattttg tgatattgcc tttctatttg
    37681 tcaaaatagt attatatttt tccatatcta cattattttc agcattcgac caaaaacttg
    37741 attcttcttt aaagtttgtt attaatttat ttaattcttt agatatactc tctatataag
    37801 cgtagttact tgtatactct aaagacaaca taggtgcatc taagtgtgat aaaaatgcat
    37861 ctggaaacac ctttcttaca ctgcttatct gctttctaac atcattttca tcaaaagagg
    37921 aataggtctg tatttttttt actccatcct tctccataac tgaaaaaggt atgtcattat
    37981 taactaatga tgtctgtatt tttttcaaat catcattatt atctattgca gcaacttgaa
    38041 tagtgtacat atcccagcct tctatagtag ctaatttgac ttcttctggc tctttagtgt
    38101 tatttaccgc tttttctgga ttagtttctt gtttatcatc tgaattggtt tgtgatttag
    38161 attttttccc tttttttatt gaatttaatt catcagatat atctttataa gttatatctg
    38221 atgaattatt tagaaatggt attttagccg atacatactc caatatcttg ctatctttta
    38281 tttttgtata agaataatag ccaattagac acaagcaagc aatcgttata acatatttac
    38341 gtaaatcaat cttatgtcta ttattattaa atcctctgta tatcgttttt cttctattca
    38401 ttagtttgtc cctcccaaaa ttatattatt aataaagtat atttttaaga gtcacaattt
    38461 atgaatataa ataattattt cttctaattt tttaataaga aattgtactt actaaacaaa
    38521 tcttcttgca ccaagtaata ctttagacca atatgaacta gttaaattat ccactttaac
    38581 cttattagga ttagtctgag taccactaca atggataaat ttactatttc ctacatatat
    38641 acctacatga ttagttgttc catcaccatc agtgtcaaaa taaactaaat ctcctggtgc
    38701 ataattcccc atagaaattg cacttccaaa attagcctgt tctctagaaa ctctaggaat
    38761 agaaacgcct acagaatgtt taaaaacata ctgtgtaaat ccagaacagt caaatttatc
    38821 aggtcctgaa gctccccatt gatatggtat tccaataaga gtatatgcaa aatctacgat
    38881 tgttgaacct tttgctgtag atttatctga atttccagta aatgatggct ttgagttact
    38941 attagattga gaattttggt tagatgatga atttggtttt tcttttgtac cttcattagt
    39001 ctcagaaata taactggcac tcacccatcc aattgttcca ctcgataact ttaccttata
    39061 ccaaccatta tttttggatt ttaattcaac aacatctcca ccatttaatt ttcctattat
    39121 agaatagctt gtcccaggac cacttcttac atttaaactc acagcagatg ttaccttacc
    39181 attcttgcct tcaattgatt cttcgctaga tggtttttta tcagaattat tagtactatt
    39241 tgaattacta gaattattcg ttccaacatc ctctgatgtt ttagagatat attggctact
    39301 agcccatcca gtaactccat ttgatagttt tattttatac catccattgc tttgttctag
    39361 taactttact acatctccat tatttgcttt accaactaat gaatagttag tacctgcacc
    39421 acttcttaca ttcagtctag aactcacatt tactttacca tttccactaa tagttgtccc
    39481 acttgaagtt ggattatttt gatttgatgt accttcagat gaaccacttg ttgatatgta
    39541 ttttgcgctt ccccatccaa caacagaact cgataccctt actttatacc atccattaga
    39601 cttttctaaa atctctacag tttttccctt gtataatttt gctaccttat cacaatcaga
    39661 acttggtcca cttcttatat tcaaggcact tgctgttact gttccctttt caagagcact
    39721 tgcatttatg gagctaacag atactgctac tgccccaatt cctaaagctg ctatagcttt
    39781 cttcactact atcactcccc ccaacaaatt tttatagtca taaattcata acattattat
    39841 ccatttttag tccctacaat ttaatgataa cttattctct tattaattgt aaagaaatta
    39901 taaacatatc cttaaaatta atgattttta ggaagtattt caatccaata attatctggg
    39961 tccgctataa aataaattcc catactttca ttttcaaaac atatgcaatc taaatcttta
    40021 tgaagttttt ttgcctcatc aaactcctca acttctaaag ccagatgaaa ttcattgtct
    40081 cctaaattat atggtctgtc ccaatctcta agccaagtta attctaatgt atgactagta
    40141 ataccatctc ctaaataaac taatataaaa cttccatctt ctgcttcctt acgctttacc
    40201 tctttaagcc ctaaagcttt tttataaaaa tctaaacttt tttctaagtt agtaacattg
    40261 aaattgttat ggcaaaaatt aaacttcatt ttgaaatcac tctccaattt ttattttttt
    40321 tatattatat cattattata aatttattta aatcatatta agttaaaaat ttgaatgatg
    40381 atgaccaatt accaaatggt ggtaaattat ttaaaagaat atgtcaaaaa aagaataagc
    40441 tctttcaaat gcacaaaaaa tttataccca agttatactt aatcatgcaa attggcagaa
    40501 cttattcttt aaaactctaa cttttaagtt tttctttgat aatataattt attacttttt
    40561 ttctaatatc ttcagcatta taatcatctt ctacttctat atccaaaagt tttgctattg
    40621 acctcatatc ttctctagtc tttgacctat agcttgatat taattcatct tttattttct
    40681 ctggttctaa aagatattct tcatctcctc ttttgaaaac aacgtatttt tgagcatatg
    40741 aacttctatt atagtatata tatcttgata tttttcttaa aatttgatta tatgaaagtc
    40801 gcttgttaat atcaagtttt aagtactctg aaaaagcaaa tagctctttt tttgatttaa
    40861 ataatgcttc tttctttata gtccttgata ttgaagttac actttcttga tatgcacact
    40921 tttttaacat ttttaaatat actctattaa gaccaatact ttgattgaat ttttttatat
    40981 tattattaat aatcttatcc aagttacctt caaagtattt ttttgctcct ttttgtgata
    41041 cataactctc aagttctttg tgggccatat caagtatcat tttttttaaa tcctctttat
    41101 atcccctaga ttccatgata ctccctccct tagctattta tagtatattc aaaaagctca
    41161 agaaatgtga gtcattacaa aattttttta ctttttatgt atatattaat ttaaaagggg
    41221 agataatagt aatgtaaaat caaacatact caatctcccc catttttaga cctctattac
    41281 tcccccaata gaggcctttt tttagtttat caatttctat tcaattccct ttagttcaaa
    41341 tcctaattct gctatcactt cttttaattt ttcaatagat atatcatctt tcatatctac
    41401 agatgcacac ttattttcta aatccacatc taaaacttca ataccatcta tatcttctgt
    41461 taatgcagtt ttaagatggt ttacacaatg accacaactc ataccttcta ctaatagttt
    41521 cttcttcatt taattacctc cttatacccc ataagggtat tctatcatat agtttatact
    41581 ttttaaatag aaattaatca tttaatttga ttcattcgat tttatatcta aattagatat
    41641 tccctcatat acatcaaaat catttggagt aattttacct tttaatctac tattacttac
    41701 aatatagttg tttctcacag ctaaacttat aaatgttgca ttatctttta tatatgtctg
    41761 tacatctttg tatgcttttt ttgtttcaat ttcagacgta gaacttgtaa gtgcagacat
    41821 atatccattt agcttatcat cagtatatcc actacttgct ataatactac ttgcatctgg
    41881 tataattgat agtttccatc caactaatgc taaatcataa tttttagaat taagagcatt
    41941 ttccatatcc ttatctgaca gctgatttac tgtactattt attcctatag cttttaagtt
    42001 ttcatttatt atgtttgcag tttttattct ctcactatta tctctgttta ctattatctt
    42061 taagttcatt tcagaaagac ttttctttac ttcctctttt tctctatttt ttttcttttc
    42121 agcttctttt ttgattctat cttcttctgc ttttttagct tttgcttctt cttctggcgt
    42181 aagcttcttt gagttctttt tagctgattt tttctgattt tttactttat tgtcattttg
    42241 atttactgag tttatatttg catattctac cttagcaaga cactctatcg ccttatcttt
    42301 attaaactct aaatccttca tttcactatt atagtatttt gattttgaat taagtggaaa
    42361 attaactgga gtagcatcat ccatataacc ttcactaata attctatctt tatctataga
    42421 gtgagcaatt gcttttctaa aattaacatc tttaaaaaat ggattattat aattaaataa
    42481 tacacactca taatctcttc cttgatattt tgttatatta aactcttttt cttgaaactt
    42541 gcttaaatca tttaatgtaa cattggttat atcactatca agagccataa ccatagatgt
    42601 ccttgcctct tcattaggca ccatacctac ttttatgttc ttcatggtct ttggaacttc
    42661 ttcatagtaa tctttgttta cagataaaac cattccttct ctttctgtat atgattctat
    42721 tttatatttc ccatttccta tcatattatt attcttgtca tttacatcct ttgaatctaa
    42781 atgattttga gatacgattg gaaaaattaa actatcaatt gaaaaagcat atctagcttt
    42841 aaattttatt gtaaaatctt tatctgattt aatattcaca gaagctatat ttttagtaaa
    42901 ttcattatat ggactatctg cacttttttg aattaaacca atagtatagt ttacatcatt
    42961 tgaagtaaca ttttttccat catgccactt tgcatctttt agctttatat ctatactcat
    43021 tccatcctga gctatattat actccttaac taattgagga attacattgt agttctcatc
    43081 tatagtaaat aaactatcat aaactaaatt cattatataa ccaacagact tatcagtatt
    43141 agttatagga tttatagttt ttggagttac cattgttaag tttatatact tagcatctat
    43201 agaactttga ctttgtaaac ttgaatcact actactttgt tttgttttag tattactaca
    43261 accagctacc ataagagtta tagctaaaac gactgttaag acttttattc tcttcattag
    43321 ttttttcctt tcttgccata tatcttatta taatgttcgt aattattttt tactttttct
    43381 acatattttg aagtttgctt aaaaggtata tcatgtagat tttcgccatc tttactatat
    43441 tcattatttt ctaaccattt tttcacatta cctgaaccac cattatatgc agctatcact
    43501 aaatctaatt taccaaattc tttgtataac ttacttaaat accaacagcc aagtcttata
    43561 ttagtctctg ggtcgaaaat atccacattt tttaaattta gttcctctgc tccccaatct
    43621 ctagttatat ctaatatttg cattaatcct tttgcttcct tttttgaaac agcagaacta
    43681 ttaaacttac tttcggcttt aataacactg taaactatat tttcatctaa attaaattct
    43741 tttgaatact tttctacata ctctgaatat tttttaggat ataaaaattt atgtattact
    43801 ttgctttcca ttaatagtgc cccaaataag attataaaaa tagatagaat caatactttc
    43861 ttactattca cgccaattct ccttcatgta ctcaataaat ttatgtgctt tactttctaa
    43921 ttcagttatt gttcccgaat tatctattat ataatcacca tatttggatt tttcttcctg
    43981 actcatctgt gaatttatcc tactaagtgc ttcctgctta ctacaattat ctctcttttg
    44041 tattctacta atttgtactt cttgtttgca agtaactact aatagcttgt caaccatatc
    44101 taaaaaacca ctttctacca atattgctgc atctaatact actatatttt cgcctttttt
    44161 tctcaattct tctatttcac ttattatttt ttctcttatt cgcggatgag tcaagctatt
    44221 taattcctta agtttttctt catcgctaaa aactattttc ccaagagctt ttctatttag
    44281 agttccatca tcatttttta tactttgccc aaaatggaca aatacttttt ccaataacaa
    44341 cttatcctca aatatcttcc tggatataat atcagcatcc actatagaaa tattaagatt
    44401 tctaaatata tttgataggc tgctttttcc acagcctatt cctcctgtaa gtcctaatat
    44461 caacataaaa tcacctactt tgtctcatac caagaactac ctacatttaa atctacatct
    44521 aagtgaacat ccatatttac agcattttcc atttcttctt ttactatttt cttaactata
    44581 tctatttcat catcaattgc ttcaactatc aactcgtcgt gaacttgtag tataagtttt
    44641 gatttcaaat tgttctcttc caatttttta tgaacattta tcatagcaat ttttattata
    44701 tctgctgcac ttccttgtat tggtgcattc attgcaaatc tctttcctct atttctttcc
    44761 ataaaattgc ttgactttat ttctggtata tatcttcttc tgtttaaaat tgttacagca
    44821 tatccatcct cactagcctt ttctactgtt gtgtccataa actcttttat tttatggtat
    44881 ttagcaaaat aactttcgat atattctttc gcctttggaa caggtatatt taaatcatca
    44941 gctagaccaa agtcactttt accatatata ataccaaagt ttacagcttt agctgcacct
    45001 ctcaattcac tagtaacatc ttccatagga acattaaaaa cttgtgaagc tgttttggta
    45061 tgaatatctt catgatgttt aaatgcttct atcatatttt catcctgact catatgtgca
    45121 agaactctta gctctacttg tgagtaatcg gcatcaacca acttacagtt atcatctgca
    45181 ataaacactt ttcttatttt tctacccatt tccattttta taggtatatt ttgaagattt
    45241 ggttctgtag atgaaattct tcctgtagtc gttatagttt gattaaatga tgaatgtatt
    45301 ctattacttt ctggatttat tatatttaaa agtccttcaa cgtaagtaga atttaacttt
    45361 actatttgtc tatactcagt tatcttatct attattggat gcttatctct taatttttct
    45421 aaaacatctg cattagttga atatccagtt ttagtttttt taattattgg aagctctaat
    45481 ttttcaaata atataacacc caattgtttt ggtgaattta tattgaatgg ctctcctgat
    45541 aattcataaa tttccttttc tagtgtagct attatttctt taaactctac actcaattca
    45601 tccaatatac ttttgtctac ttttatacct tcatactcca tatgacctaa gacttctact
    45661 aaaggcatct ctacatgata aaaaagacca tccatactca tatccattag acttctttcc
    45721 atcataggaa ttgtttcttt aactgtgcat attatttgac ccatatactc tgctaaatcc
    45781 tcaaattcta aatcctcata gtttttagat ttaacaccct ttcctaataa ttcttctttt
    45841 gatttaacct tttttgataa atacttcata gctattgcac tacaatcata tgaggtagat
    45901 gaagttgaat ctattaagta ctcagctatt gttatatcaa aatacatatt ttctaagttt
    45961 atattatatg gttttaaaga tatataatca tcttttaaat tatatccata ctttttaatt
    46021 tcattatttg aaaatatact atctaacttt gacacttcat ttgaatctac ataatatatt
    46081 ttttctccat ctacacttat aaaaatatat attatatttt tttcaagtat attaccttgt
    46141 cctctaacag tttttaaaat aacttgtcct tttttgttta cttcatctat aaatttttct
    46201 gtatctttta attttaatat ttctattttt tcttctacgt gttctgtgtc atctgaatcg
    46261 accaaatcaa ctaatcttga taagagactt gtaaatccaa agtatctaaa tttatctaaa
    46321 agttcatctt tatcataatc gccaaaaacc atactatcta aatccacttc tattggcaca
    46381 tctcttataa ttgttgcaag tcttttactt tgaatagcta tttctttatt ttcctctatt
    46441 ttctttttta cactaccttt aagcttatct gtattctcta ttaaattctc tatactagaa
    46501 aactctttta taagctttat acctgttttt tcaccgattc caggtactcc aggtatatta
    46561 tctgacttat ctcccataag accttttaaa tcaataaatt gtgctggagt catctcgtat
    46621 ttttcaatta cagaattaaa gttgtattct tcaacttctc caactccttt ttttgttata
    46681 agagtagttg tattatctga agcaagttga atagcatctt tgtctccagt tactatgtat
    46741 accttatacc cattattttc tgctttttta gaaacagttc ctataatgtc gtctgcctca
    46801 tacccatcta tttccaatct atttattttg aatttatcta aaaggtcttt taatggctgt
    46861 aattgttctg ccagttcatc tggcattccc tttcttccag ccttatactc tttatattct
    46921 aaatgtctaa atgtaggtgc ttttctgtca aaggctacac ttatatgtgt tggtttatat
    46981 atatctatca ttttaaatag cattgttgta aatccatata ttgcattggt ctttaaccct
    47041 tctttattat tcatttctgg cagagcataa aaggctctgt ttattattga atttccatct
    47101 attataatta attttttttc caaattatat cactcctaaa aattatctat ttttgtaaca
    47161 tatatattat ataccaatat agtgttattt aatattccca taataataat agtatattaa
    47221 aattaaatat gttgcaaaca atatattttt ttgctataat aatatacatg acatgcccga
    47281 atggttgaat tggtaaatac aatggattca aaatcaactg tgttgattca taagttttta
    47341 taatgtttta cagtttgatg agtattttgt attcaatatt cctcttgcat attcttggaa
    47401 aatctggtag gatatacaag tagtttggat ttaaaatata agccgctgtg gcggaactgg
    47461 catacgcata cgactcaaaa tcgtacggga aaccatatgg gttcgagtcc catcagcggc
    47521 accaataaaa aaaactcttt agtatatact aaagagtttt tttattgtaa tattctttca
    47581 tctttatttc tagccttgta taagtataaa taccatagtc ataacttctt gaactcctaa
    47641 aatatatcta aacaaagtag gattttttat gtattcactt aataaatcca tacttcttat
    47701 catattgatt ttttcccaat ctctaaaaat tttgatacaa ataaaaaata cagagaacta
    47761 cttgatatac tagaaggtta ttctgtacca tttggtattg ctgtaggtgt tccagatgga
    47821 aatttaccaa atgctagaga tgtagtttat aaagtttcta gagtttagga taaagtgttg
    47881 caacaatcaa ttagttactt gaagagttaa atcagtaata tacaaataaa ttaatggaag
    47941 aggaagctat ttaaaaagta gtttagattt ttcattgtaa
//
LOCUS       DAGQWR010000008        46695 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.008, whole genome shotgun sequence.
ACCESSION   DAGQWR010000008 DAGQWR010000000
VERSION     DAGQWR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.008"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            46..120
                     /locus_tag="MIW95_000356"
     tRNA            46..120
                     /locus_tag="MIW95_000356"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78..80,aa:Thr,seq:tgt)
     gene            complement(280..957)
                     /gene="srtB"
                     /locus_tag="MIW95_000357"
     CDS             complement(280..957)
                     /gene="srtB"
                     /locus_tag="MIW95_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBY3149715.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKTSSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKNNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            1089..1423
                     /gene="rnpB"
                     /locus_tag="MIW95_000358"
     ncRNA           1089..1423
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="MIW95_000358"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1701..4244
                     /locus_tag="MIW95_000359"
     CDS             1701..4244
                     /locus_tag="MIW95_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBY3149716.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGAKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQAKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            4366..6357
                     /locus_tag="MIW95_000360"
     CDS             4366..6357
                     /locus_tag="MIW95_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149717.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCDIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            6347..6667
                     /locus_tag="MIW95_000361"
     CDS             6347..6667
                     /locus_tag="MIW95_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149718.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            6865..7734
                     /gene="galU"
                     /locus_tag="MIW95_000362"
     CDS             6865..7734
                     /gene="galU"
                     /locus_tag="MIW95_000362"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBY3149719.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            7759..8772
                     /gene="galE"
                     /locus_tag="MIW95_000363"
     CDS             7759..8772
                     /gene="galE"
                     /locus_tag="MIW95_000363"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBY3149720.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            8884..10818
                     /locus_tag="MIW95_000364"
     CDS             8884..10818
                     /locus_tag="MIW95_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBY3149721.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKAINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLSV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSLEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            10973..11836
                     /locus_tag="MIW95_000365"
     CDS             10973..11836
                     /locus_tag="MIW95_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBY3149722.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(12044..12925)
                     /locus_tag="MIW95_000366"
     CDS             complement(12044..12925)
                     /locus_tag="MIW95_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY3149723.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            13338..14186
                     /locus_tag="MIW95_000367"
     CDS             13338..14186
                     /locus_tag="MIW95_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBY3149724.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            14265..16187
                     /locus_tag="MIW95_000368"
     CDS             14265..16187
                     /locus_tag="MIW95_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBY3149725.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDIHDSSVIAAYLEGCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            16269..17132
                     /gene="aroE"
                     /locus_tag="MIW95_000369"
     CDS             16269..17132
                     /gene="aroE"
                     /locus_tag="MIW95_000369"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBY3149726.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGIEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNAKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            17332..18549
                     /locus_tag="MIW95_000370"
     CDS             17332..18549
                     /locus_tag="MIW95_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3149727.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLIVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            18785..20650
                     /locus_tag="MIW95_000371"
     CDS             18785..20650
                     /locus_tag="MIW95_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBY3149728.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIEYNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(20828..22192)
                     /locus_tag="MIW95_000372"
     CDS             complement(20828..22192)
                     /locus_tag="MIW95_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY3149729.1"
                     /translation="MSQLLIKNAYLITMNADREVYETGSILIENNIIKNVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLIRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHNPGAAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(22339..23682)
                     /locus_tag="MIW95_000373"
     CDS             complement(22339..23682)
                     /locus_tag="MIW95_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBY3149730.1"
                     /translation="MVPNTNNVQPDGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(23755..23856)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24186..24839
                     /locus_tag="MIW95_000374"
     CDS             24186..24839
                     /locus_tag="MIW95_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBY3149731.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNSFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDEIRGAIFDVSQAIDLTKLMGIIIK"
     gene            24980..26251
                     /gene="brnQ"
                     /locus_tag="MIW95_000375"
     CDS             24980..26251
                     /gene="brnQ"
                     /locus_tag="MIW95_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBY3149732.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAIFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            26453..27640
                     /locus_tag="MIW95_000376"
     CDS             26453..27640
                     /locus_tag="MIW95_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBY3149733.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMTKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(27870..29894)
                     /locus_tag="MIW95_000377"
     CDS             complement(27870..29894)
                     /locus_tag="MIW95_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3149734.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGEVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKRAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            30284..30952
                     /locus_tag="MIW95_000378"
     CDS             30284..30952
                     /locus_tag="MIW95_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149735.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLASNFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            30954..31439
                     /locus_tag="MIW95_000379"
     CDS             30954..31439
                     /locus_tag="MIW95_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBY3149736.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            31500..32621
                     /locus_tag="MIW95_000380"
     CDS             31500..32621
                     /locus_tag="MIW95_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBY3149737.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMQFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            32902..34092
                     /locus_tag="MIW95_000381"
     CDS             32902..34092
                     /locus_tag="MIW95_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY3149738.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDIVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLVRKGFKH
                     TPLQSLCPEYKDKIITCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    34226..34481
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            34540..35541
                     /locus_tag="MIW95_000382"
     CDS             34540..35541
                     /locus_tag="MIW95_000382"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBY3149739.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            35645..36034
                     /locus_tag="MIW95_000383"
     CDS             35645..36034
                     /locus_tag="MIW95_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBY3149740.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIIKKDKHSNIIENVEFSIKDEEE"
     gene            36195..37403
                     /locus_tag="MIW95_000384"
     CDS             36195..37403
                     /locus_tag="MIW95_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBY3149741.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(37490..38566)
                     /locus_tag="MIW95_000385"
     CDS             complement(37490..38566)
                     /locus_tag="MIW95_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY3149742.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            38795..39322
                     /gene="hpt"
                     /locus_tag="MIW95_000386"
     CDS             38795..39322
                     /gene="hpt"
                     /locus_tag="MIW95_000386"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBY3149743.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            39411..40343
                     /locus_tag="MIW95_000387"
     CDS             39411..40343
                     /locus_tag="MIW95_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBY3149744.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIVGWMLGIFFTVIF
                     IKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            40405..41631
                     /locus_tag="MIW95_000388"
     CDS             40405..41631
                     /locus_tag="MIW95_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBY3149745.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINANTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            41715..41936
                     /locus_tag="MIW95_000389"
     CDS             41715..41936
                     /locus_tag="MIW95_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBY3149746.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            42125..42295
                     /locus_tag="MIW95_000390"
     CDS             42125..42295
                     /locus_tag="MIW95_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149747.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(42388..42849)
                     /locus_tag="MIW95_000391"
     CDS             complement(42388..42849)
                     /locus_tag="MIW95_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149748.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQHFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            43032..43499
                     /locus_tag="MIW95_000392"
     CDS             43032..43499
                     /locus_tag="MIW95_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149749.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMIAFNLNMILGIGVFLIGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            43542..45305
                     /locus_tag="MIW95_000393"
     CDS             43542..45305
                     /locus_tag="MIW95_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBY3149750.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDTIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMKDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            45313..45921
                     /locus_tag="MIW95_000394"
     CDS             45313..45921
                     /locus_tag="MIW95_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY3149751.1"
                     /translation="MDSNDKKGLKSTEIIIINGSPRINKNCSFIANKVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRNKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            45993..46562
                     /locus_tag="MIW95_000395"
     CDS             45993..46562
                     /locus_tag="MIW95_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBY3149752.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT        18122 a         5101 c         8033 g        15439 t
ORIGIN      
        1 taacttttaa aaattagttt tactaattta caatagaata ggtgtgctgg tgtggctcaa
       61 cggtagagca gctgacttgt aatcagcagg ttgtaggttc gattcctatc accagctcca
      121 gtaaagacta tgtttttagc atagtctttt tttattgttt tttattgttt gatgtatttg
      181 ttattttgta ttacatactt ttagtggcct aatttataaa ttataggtat aaaagatgac
      241 acagtttaaa attattgttt taaactgtgt ctgtgagttt taaatcaatc taccatgaat
      301 caccattctg gcatcatcaa attcataggt acatgtagaa agtgtaacta ttttatcatt
      361 gctatttacc ttaataggag atttatacaa tgatttagaa gtaatatcat ttatgtaatt
      421 ttgataatca gattcattgt taaaatttgt ttttagataa tcataatcag attctacaat
      481 ataagcagaa aaaacatcat ataaaaattc ttttccattc aaggttattt ttattttatt
      541 attgttttta aagaagtcag catctttaaa cttatttagg ttgttgaaca tagttttatt
      601 tttcatatta tgtccatata ttatgatatt tttgtcatca atagatttat ttctataatc
      661 cataaacagt gtaccagaaa tagaatcttt tttataaaaa tctttgtcaa gatagtaaga
      721 attatcttta gattgtacaa ctggataatt tatatttgta ttctcaacag aaagccaaaa
      781 tttaaaatca ctgttaatct ttgataaatc ctctttttta tattcttttt tttgaagttc
      841 tgaggatgtt ttagtatcat gattgtattt ggtaagtttt gagtatatat taaatccact
      901 gtaaagtatt actaatacta gtataatgtt aattactatt ctatataatt ttttcaatat
      961 atcacctcga gttaaatgga gtttatatat ctatattaca ctatttttga ataaatttaa
     1021 atttttagta taatattact ttgacatgta aaagaatttg tgttaacata actaagtaaa
     1081 ataaatatta agtaagccag ataatcgcat gtaatcttac atgaggaaag tcggagctcc
     1141 atagagcagg gtgctaggta acgcctagtg ggagcgatcc taaggatagt gcaacagaaa
     1201 taaaccgcca ctattgtggt aaggatggaa aggcgaggta agagctcacc agcagctagg
     1261 cgactagttg gctatgtaaa ccccacctgg agcaagacca agataggaat aaagagtgct
     1321 gctcgtactc tccggtaggt aggtcgcttg agcctattgg tgacaatagg cctagaaaga
     1381 tgattatcta atacagaact ccgcttatag acttgcttaa ttatttcgat agtcaaaaac
     1441 atcagtttaa actgatgttt ttttatttgt aaatttgtct aatctacttt taaaattttg
     1501 aggaatattg taatataagt tagtacagat attaaaaatg gtataatata agatagatat
     1561 aaaaagaaaa agtaaaatga tgtaaataat atgatatgat ataagatgta gcaaatttag
     1621 taaataatta tttagatatc ataatcaaat tagcaacttt gcacaatgta aatattacaa
     1681 taaaataatt gatttaagga gtgaaggttt tgagtgaaaa taatataata agaaacactg
     1741 ttttaaatgt cacagcaagt tttttaaaac aagagtctaa aataaatgaa aagctagatg
     1801 gtgttttgga aaagaaattt gaaaaagtag aatttaatga agcaaaatat gcagagcttt
     1861 taaaatttaa tatattgttt tataagacat tagcaagaaa tacagaacca cttattggca
     1921 aatggatagt agataagtac atacctgaaa tagatgaatt agaaaaagat ttagaattaa
     1981 ctacagcaaa atgtagaaag tatgtaaata aggctatgaa agagggatta gactctctaa
     2041 aagccaatga tttaaatagt tttttagctt atgataaaat ggaactatca gagagaagaa
     2101 gacgtttaga aaaggattat aaggttttaa acctatataa agacctcctt aatatatctc
     2161 ttagaaaaat aagtttagaa aagaaagaat gtggagattt atttcttaaa aatcaagcag
     2221 atgcgaaaag agaattaaaa agagaaatta tattctgtgt aaacaaaata ttagctagca
     2281 ataaagatgt ggtacctaat aatgtggaag agaataacga acaagacaaa gtagaaataa
     2341 aagcaactga aattgaggat ttgcaattag gtaaagataa ttcagaaaat gacatgctac
     2401 taaatgaaat taaaaattca aaagtaaaac atgaatcaaa atcaaatgat atagaatcga
     2461 agtttataga taagttaaat agcttaaata atgaaagtat atctcaaaca atacacaatg
     2521 aatataagaa gttatgtgga ttagaagaac ttcattctgt tgaaggatat ggttttggca
     2581 aagaaattat taaagatttt gcttgtgcaa cggttgtatt agaattttta aagagaagaa
     2641 atagagacct tatagaaggt gcaatgagac tgactataat tggagaattt ggtcctgaaa
     2701 attttaagga atttgtggac tatgtaatta aaaataaaac agaaattagt gaagatacat
     2761 ggaataaagc acagaatcta ataaaagata actatagtga attagaaaat catgaaattg
     2821 catcaaaaag aactagaaga aataaagata tcgatattga agaatacatt tacatgataa
     2881 aaaatgcaga taaagatatt tgctttagaa gtagtatttc aatagaagat gatgctgaag
     2941 aaggggctaa agaagaagta gatagtaata atcaagatat aggtgacgtt gtagaagata
     3001 aagatactac agataaagag tacgacagca ataaagaaga tataattgaa ccagaaaata
     3061 aaaagagtaa aaagaaggct aaattatttg gatttattaa aaaagacaat gaagaagttg
     3121 aacaagaaga agagaattta aatgatattt ctcctgatat aattttagat aaaccagttg
     3181 aaaatcatca agcaaaatca gaagaaatag agcaaaatga attaaaagaa ataaaacaag
     3241 aagaaccaag tcaacatata gaagaagaac gtagtgtaaa aatagaaaaa ccaataaata
     3301 ataacttaga tgaaaaagta tcttcaaata atgaatcgaa gcttgaaaaa gaaagtaaaa
     3361 acttagaaga taaaaaagca aaagaaataa aagaagaaaa attagaaaat gaaaaatcag
     3421 tagtaatacc tattaaaaag aaagaaaata gtaataaaaa atctaaaaat tcttctaagg
     3481 acaaatatag agaaaacaag aaagaaatga aaaattatgt atcagataaa gaagattctt
     3541 tagatgatga agaggttgtt tctaagaaga gtagattaaa agaaactatt atagcagtag
     3601 ttatagtggt tattgttggt gtaggatatt ttataacagt aggcaacaat aagaaaaatg
     3661 ataaagagaa tattccaaag tcatcaactc aacaacaagc taacaataaa ttaactgagg
     3721 aagagaaaaa agcacaagct gaaaaagaga aaaaagaagc agaagaaaag gcaaaagctg
     3781 aaaaagaggc tcaagaaaaa gcacaagctg aaaagaaagc aaaagaaatg gaagcatata
     3841 aagatggtaa aggtgtatat tacactgttt atgcaggttc acttaaagtt gaaaaaacag
     3901 caaaagaaac agcaaaagaa tatgaggcta aaggtatttc ttcaacaata attcaagaaa
     3961 atggatacta taaaataaaa ataggtgatt attctcaata tggagaagca caagaaaaat
     4021 gtaatgaact agctaagaaa tctattgata catatatagc tatgtatgat aagtactatg
     4081 attataagtt agaagaatta aaagaatcag ctccttcttt gagtgctgaa gaacttaaac
     4141 aaaaatatga agatttaaga agtgagttaa agaacaaatc tggatatcgt gaatatgtta
     4201 aacatttaga taaactttat gaagaaatag ttgaaggtgc ttagagcatt ataaaaaagg
     4261 agaaactatg tctcagaata aaaagtatta aaaaaatatt atttgttttg agacatttta
     4321 ttcaccttta taaaatactt ataattctgt ttaggtggtg aaggtatgtt gaaattagga
     4381 gaaaaaatca tatatgagct ttctgatggg tttgagctta gtggcataag tagatactca
     4441 aaagaatata aaaagcttca tataacaaat ctagggaata taataatcag caaatcagat
     4501 gaagttaatg aggatacagg tgtaaaatat atatatagtt tttcagacaa taaagaaaaa
     4561 ttagaggcag ttttagaaga ttctcaaata attgtctatg gaaataactt gcctttttac
     4621 ataaaaagtg aaaattcaag taaaaatgtt acagcaaatt tgactatgaa aataactttg
     4681 gatgaatatg atttaatctg caaaagtcaa agagaattta tattctatct aaaggataat
     4741 atgactatat taagtttaga tgacaataag ttttatatgg gtggaattaa taaagataat
     4801 gagaagttta tatttatcag tgggaaaaat agatttgaaa taaattttga tgatatagaa
     4861 agatacattt tagaagataa aagagtgtca ttgaagggat actttcatat ggaaagagag
     4921 gggattatag taaggtctgt atctatattt aataacaata tagatagtgt agttccatct
     4981 gatttaaatg agagagttaa agataaccaa aaaataggaa atcttcctaa agattgtgat
     5041 atagtattct gcaagatatc aggaaatata gatggatttg attataaaaa cacaaacatg
     5101 cttttagtaa aatatcaaga ccaattaatt tttataaata agaaatctaa gaagactatt
     5161 gtaaagtcag cgaaagataa ttgttctaag ttaaatttag gagaagatat aatattatat
     5221 gataataaaa atgtatttaa cctacatata aatgataaaa atagagaaat tatgcaaata
     5281 gatgacttaa aagatataga aaatgaaata gtgggatata cattaaaaca tgctccattt
     5341 tttattcaag aagatttcga tagcttgact attctaaagt cttttcaaaa agaaatcata
     5401 tctataaaaa atagtgatat aaaagacatt gtaattaata aagaacttga aaacgaaaat
     5461 tctaattttg tagaaacgga aataaaattt aataatcaaa aagtactttt aaacttaagt
     5521 aaaaatatgg ttcaaaaact tatgcaagat gtatttatat atgcaaagca acctctactt
     5581 aaagaaaatt ctattgaagt gatatataag aattggtcta aagctatgaa tgatatgata
     5641 atatttaatt tttttggaaa tatttactat atgaaatctg aatttgataa gattttagaa
     5701 aaagagttaa atgatgaaat aagaatagaa gttataaatt cactttataa gcaaatacaa
     5761 gaacaaagaa acaatttaga tttattgtca gcatatatgc caagaatatt agaaaatcaa
     5821 gaaatagatt tgtttgaaaa gtataatact aaacttgatg tacaagtgtt taagcagatt
     5881 aaaaatctat tgtctgactt atcatataat ataagcagtt atttaaatga agttgaaaaa
     5941 agtttagaca atatcatctt tgtaatttca ggagaagata agaagaaata caattataga
     6001 atgttaaaag aaagtgaaag tgcaagtctt gatgtatttt taaaacaagc catatcaaga
     6061 ttaaaccatt tagtagaaaa tatgtatcct tattatgtag atgaaactag tagggaaatg
     6121 tttaagttat ttgagttatt gtggaaaaac tatagaaata ttgatgatga tagtataaaa
     6181 gaaatattat tcgagagaat aacaaataca tatgtattta aacaattgac attaaataat
     6241 agtacaaaag aaagaagaaa agacataatt gaaaaaatat ataactctgt tgattatgga
     6301 accaataaac tagatgaaaa tatgtttttc acaggaggaa taaagtatgt caagtaaata
     6361 caatgtttgg acttttaact atgagttctt aggattggac tcaggggatg aaaaaaataa
     6421 tgaagtttat tacgagattg atttagatga aattttaaat gaaaactata tagatgaaga
     6481 tatagatagt ggtttagatt ctgatggaaa tagtatttta ggagaattaa tatcagaaat
     6541 ggatatagat gaggatgtca gtgttgatat gacatatcaa gaactagaag ttgacttaga
     6601 agatttagat tcttatctag atgaagatat agatagtgat ataaaaggca tcttagatga
     6661 aatttagtac ctgtataagt tttggataaa atataaacaa tacaatatta acttataaaa
     6721 ctaaaggtag atgaaatttc atctacctta tattatctta tagcattaaa tattgggata
     6781 atatagagtt tgaagaaaaa gtataaatat ggtatattaa tttggtacaa tagaaaattt
     6841 acgaagaagg gtgaaaaaat gcagatgaca gttaaaaaag caataatacc agcagcaggg
     6901 ctaggaacca gatttttacc agcaacaaaa tcacagccca aagaaatgct tcctatagta
     6961 gataaaccaa ctttacagta tataatagaa gaagctatag aatcgggaat tgaagaaatt
     7021 cttataataa caggtagaaa taaaaaatct atagaagatc attttgataa atcagttgaa
     7081 ttagagttag aactagagca aaaaggtaag acagagatgt tagaaatggt aagagacata
     7141 tcaaatatgg taaatattca ttatataaga caaaaagagc caaaaggtct tggacatgca
     7201 atttactgtg caaaaagttt tataggtgat gaaccttttg ccgtattatt aggtgatgat
     7261 atagtagatt cagatgttcc gtgcttaaaa caattaatag atacttataa tgagtataaa
     7321 acaactgtat taggtgttca aaagatagct aaagaagata ctaataaata tggtatattg
     7381 gatgtaaaac atatcgaaga tagagtatat aaagttaaag atatggttga aaaacctgca
     7441 attgaagaag ctccatctga tatagctata cttggaagat atataataac tcctgcaata
     7501 tttagtatat tagagaaaca gacacctggt aaaggtggag agatacaact gacagatgca
     7561 cttcaaactc ttggaaaaca agaggctatt tatgcttata actttgaagg tagaagatat
     7621 gatgtgggag ataagattgg ttttttagag gcaacaatag actttgctct aaagagagaa
     7681 aatttaaaag atgatttaat ggactatatg agaaaaaaag taagtgaaaa ataaaatatt
     7741 agaaaagagg taattacaat ggcagtttta gtagctggag gagcaggata tataggaagc
     7801 catacagcaa tagaactttt agaatcaggt tatgaagttg ttatagttga taacttaagt
     7861 aatagcaact caatagtggt tgatagaatt aaggaattat ctaaaaaacc agtaaaattt
     7921 tataatattg atatcagaaa taaagatgaa atgcatgttg tatttaaaga aaataatatt
     7981 gaatcaatta tacactttgc agctttaaaa gcagttggtg aatcagtaga aaaaccaatt
     8041 gaatattaca gcaataatct aataagtacc ttaaatttat ttgaattaat gagagaatat
     8101 ggtgttaaaa agtttgtatt tagttcttca gctactgtat atggagaccc acatacttgt
     8161 cctatattag aggatttccc actttctgta accaatccat atggaagaac taaacttatg
     8221 atagagcaga tgttagtaga tatatcaaaa gcagataagt cattggatat agctctttta
     8281 agatacttta atccagttgg tgctcataaa agtggtagaa taggtgaaga accaaatgga
     8341 gttccgagca atctaatgcc ttatataaca aaaatagcag taggtaaatt gaaggaattg
     8401 agtgtttatg gtaatgatta tccaacacat gatggaactg gagttagaga ctatatacat
     8461 gtattagatt tagctgctgg tcatgtaaaa gcattacaaa aattagaaga aaatccagga
     8521 cttgttgttt ataatttagg aactggaaag ggttatagtg ttcttgattt agtaaaagca
     8581 ttttctaaag caagtggaaa agaaatacca tataagattg taggaagaag ggctggagat
     8641 gtagccatgt gttatgcaga ttcttcaaag gcagaaaaag agcttggatg gaaagctaag
     8701 tatgaacttg aagagatgtg tgaagattca tggagatggc aaagtatgaa tcctaatggg
     8761 tatgaagagt agaatacata cttatatgta taaatgtaac aaatttgtaa atataaggta
     8821 ttggtaattt tatgttataa ttagacacat gtagtaatgt ttctaaaaga gggggaagtt
     8881 atagtgaaaa aaagtctaaa aaagtattta gtactagcac tgactttagt tttagtattt
     8941 gcatgtggcg taccagaatc aagcgcagcc agtaaacatg tgattatagt aaattcaaga
     9001 aagaatactc taggatattt tgtaaataat aaacttgtaa aagaatttag agttgcaaca
     9061 ggtaaaaagg ggtcagaaac accaacaggg aaaacaaaag tagttaacaa gataaaaaat
     9121 agaccatatt ataaaggtaa tatacctgga ggaagtccaa gaaatccatt aggagataga
     9181 tggatgggat tagcattaaa aggaacttat ggagatacat atggaataca tggaaataat
     9241 aatgagagtt ctataggaaa gcatatttct ggtggatgta taagaatgca caataaagat
     9301 gttagatggt tatttgacca agttcctgta ggttcagatg taattataga ttatagtaat
     9361 gacagctatg taaagatagc ggcaaaatat aagataaatc taaatcaaac aggatggaaa
     9421 acagaaaatg gtaagaagta ttatgtaaaa tctgatggaa cataccaaaa gaatagttgg
     9481 ctaaaagtaa atggaaaaat gtattacttt gatgcttcag gagtaatgca aacaggatgg
     9541 aaagcaatta ataacaaaaa atattattta ggaactgatg gagcaagagt atctggatgg
     9601 aaagtaatag acggaaaaac ttattacttt aactcagatg gagtaatgca aacaggatgg
     9661 caagaaaaga atggaaagaa atactatcta ggatctgatg gtttatcagt aacaggatgg
     9721 caagaaatag atggtaataa gtattacttt gacaaaacag gaataatgca gacaggatgg
     9781 caacaaatag atggtaagtc ttactattta gacaaagatg gaaagatgct aactggttct
     9841 caaaaaatag atgggaaaga ttatactttt aatgaagatg gaactataaa tcctacatgg
     9901 gatacaatta taggtgcaaa tagatttgat acagctaaaa agataagttc agttggaaac
     9961 tggaatgctg atagttcaga tactgtaata ttagtaaatg gtaatgcaat agctgatggt
    10021 ataacagcaa ctcctttagc aagtagctat gactcaacaa tactacttac taatacagct
    10081 aatttaccaa ctgaaactgt tgaagaaatg aagttattag cacctaaaac agtaatcttg
    10141 ataggtggag aaaatgcaat aagttctaaa ttagagcaag aaataaaaac tactttcaac
    10201 acagagacaa agagaatagc aggtcaagat agatatcaaa ctgctacaag aatagctgaa
    10261 gaacttggaa atcgagaaga aataaaaact gcatatatgg tttctggaaa tggggaagca
    10321 gatgctttat ctgtagcttc taaagctggg gaagaaaaac aacctataat attagtcaat
    10381 aaagatggaa ttacagaaga atcttataaa tggctaacag aaagaaaatt agaaaatgca
    10441 tactttatag gtggaccaag tgcaattaac gattcagtaa tagcaaaaat gaatgatata
    10501 actactgaag atatttcagg aaatagaata tatggagata gtagagtaga tactaatgcc
    10561 aaagtaatag aaaaattcta tggagatact gatttacagg ctgtattagt tagtaaatca
    10621 gatgctttag tagatgcttt atcagctgga ccattagctg taaaattaca ttcaccaata
    10681 gttttaatgg ataattcagg attatcatta gagcaacaaa gagtatttgc aaataaaaaa
    10741 gttgaaactc cttatcaaat aggaggcgga gtatcttata tagtaatgga taaattaatg
    10801 gatattcttg caaaatagta aaaatatata ttgaaaaata attataattg agatgtgaaa
    10861 ttcaatcaat aaaagcttga ttgttttaca tctcaatttt ttagtataaa attaagttta
    10921 agttatataa aaatcttgga atatttttaa aactcatata aaatgaatat agatgctatc
    10981 atcatatgtt aagagggtga gtaagattat aaagaaaaaa tatataatgt ttattgatga
    11041 aagtggaaga gctgatataa atcatgaaga cccatttaca ttgacaggag taatttttga
    11101 atataaatat gcagtaagtc aaggcgatta tgtatgttct ttgaggaaag aaatggatgc
    11161 atttaaagag tattgttttg gaaccagtaa cataggaata catttgacag atatatcaag
    11221 aaaatcaaga gcatttgaga agtttgatga ttcacagatt aagttattct atgatgagtt
    11281 gcctttattt ctatctagat tagagtgtac tataatatca gtaacagtag ataaagctag
    11341 attaaagaaa tattatgcac cttcaaagga accgtatgtt gtggctttta tacatgttct
    11401 tcagaatttt tattcatttg taaataatga aactgtagaa tctgcacgaa tagttataga
    11461 gtcaagggat gatgtctcaa acttaaaagt acaaaaggcg ttttttgatg tatttaataa
    11521 tggaacaata tacttagata tagatgaaga attaagagat aaggttaagg gttttataat
    11581 tgccaaaaaa gaagatgctg actatagaga aggtttagaa atagcagata ttttatgtaa
    11641 tcctcttagc agagcaagaa gagggttaat agaagctaat ccaaagtgta tgcaatatgg
    11701 agaaaaaaac aaaatattca aatctgtcag aaacaaaata tatactccaa cgagtgtgaa
    11761 tgatataaga aattggggat ttaaagaagt tcctattgta gagcctgttg gaccatggct
    11821 tgaaaaaatg cgttagatac aattgacaaa tttaaaaaaa tctgatataa tgtaaataac
    11881 ctagttattc aggaactgaa tccagttgct gttgtataac gataattact gagactcgga
    11941 tgccgactgt cagttaggaa tctatacttt taaaagtata gattttttta ttttataaaa
    12001 agaataaaag aggatattta aaaataaccc ctttattaat aaattataat tgagcgtttt
    12061 taatgacaaa gtttttaaag tttttaattg caggaggcaa atatctatct tttataaatg
    12121 ctaagtaaaa gtttctttcc caacttgggt gaataatctg aattactttt acttttgtaa
    12181 agctaagcat attcatattt gggactacag caattccgaa attttttgca actaatccag
    12241 caattacttg gtcttcctct acttcataga ctatattagg ctgttgattt atttttttaa
    12301 acatatcatc aattatatat ctaagaccac ttctctgatt aaatactatt tgaggataag
    12361 gtatggtttc ttttaaatct atagtatcct ttgcagctag tggatgacta tatggtacaa
    12421 ttagtactaa atcttgtttg gcaacaggta taaattcaat tcccttttct ttttcaagtt
    12481 tcgaacaaaa tgcaatatca tattttcttt ctttaagacc ttgaataatg tcagtagtta
    12541 caccagtatg aaatttaaaa tctattgatt tagcaggatt agattttaaa aatttatgca
    12601 ctatgtctgg gataaaatca gtaccaagtg ttctaagaaa agctaagtct atttgtcctt
    12661 caccagtacc agttattttt aatgatttta cacttgaatc taaaatttct aatgattttt
    12721 ccacatcttt taaaaatatt tttccatact tagtaaggac tatatttcta ccttcttttt
    12781 caaatagagc caccccaagt tcattttcta gtaaagaaat agcatgactt aagcttggtt
    12841 gtgttataga taaattttct gccgcttttg tatagtgttc taaatgtgcc aatgtaacaa
    12901 aatagcgtaa gtgatataaa ttcaattttt gttcatcctt tcataggtat ttttatttat
    12961 atatttgtag aagttaacct ttaagatatt tttacatttt tattatatat gattatagag
    13021 taagaataaa agcttttaaa aaaatttatc gttttttgtc tgaattataa gataaatata
    13081 tttatacaac ctaatagaaa aatacaagaa ttatcttata aataaaatct ataaacaaaa
    13141 tctatgaatt aataggaaat tatcaatttg ttattagtaa agtctcgtga tacaataaat
    13201 ttataaaatc tagataaata gaaatcaaaa aatactttta tttttaaggt aaaataatac
    13261 tactagtagg ataaataaaa atataaaatc aaagatacta ttaattataa agaaaataaa
    13321 gggggcaaat ttaagctatg aataatcaaa aaaatcttaa tattccaatt acaattagtt
    13381 cttatacatt gggaacagaa gtttcattcc gtgatagagt tcgtattgca aaagaagctg
    13441 gatttgatgg tattggattg cgtgctgaaa attatattga tgcaaaaaat gcaggtgtta
    13501 cagatgaaga aatgattgca attcttaatg aatatgatat gaaagttaca gaagtagaat
    13561 atataactca atggggaaca gaagaagata gaactgaagc tcaaagagaa aaagagcaaa
    13621 atgtttatca tatggctcac ttatttaatg ttaaacatat aaattgtgga ttattagaga
    13681 tgataccaga agaacaaatt ataactgcat tgggagaatt atgtgacaga gcaggtgaac
    13741 ttattatagg tcttgagttt atgccatata gtggtgtacc agatttggct actgcatggc
    13801 gtgttgtgaa aggatgtaat cgtgaaaatg caatgcttat attagatact tggcactggg
    13861 ctagagcaaa gcagacagca gaggaccttg cacctgttcc agcagataag atagtatcaa
    13921 ttcaagtgtg tgatgtttta gaaactcctt atgaaaaact tagggatgaa tcactacatg
    13981 accgtttggc tccaggtgaa ggatatggaa acacagagga atttgtgaaa ataataaaag
    14041 aacacaatat atcaccaaga gtcatagggg cagaagtaat atctgattct ctaatatcta
    14101 aagggttatc cactgctgct gaaactgtat ttaatgcaac taaaaaagtt ttggataaag
    14161 catggtctga agtatctcca aaataaactt atataatata aaaatataca gagtattata
    14221 atataaaact tttaaatata aaacttgaaa ggaaggaaaa ccttatgaag attagttcta
    14281 tgtttacacc tataagaatt ggttcaatga cagttccaaa tcgttttgta gttcccccta
    14341 tgggaaacaa ttttgcaaac acagacggta ctttaagtga aacttctaag gcttattatc
    14401 ttgagcgtgc tttaggtggt tttggattaa taactataga atctagtgtt gtagacaaaa
    14461 aagcaaaagg aggtcctcgt aagccttgtt tatatgatga tagcacaata gatagtttta
    14521 aaaatgtaat agatgcttgt catgatgcag gttcgaaggt atctatacaa ttacaacatg
    14581 ctggttctga aggaaatgaa aaagtagcag gacatccatt aaaagctgct tctgcaatac
    14641 ctgcatcaaa tggtcgtaat acaccactag caataacaac agaagaaata tatgaactta
    14701 tagaatctta tggagatgct gcattacgtg cacaaaaagc tggtgcagat gctgttgaag
    14761 ttcactgcgc tcatggatat ctggttagta gttttatatc acaaagaaca aataagcgtg
    14821 tagatgagtt tggtggatgt tttgaaaata gaatgagatt acctcgtctt atcattgaaa
    14881 atatacgtaa aaaagtagga aattctctag caatattatg tcgtataaac agcactgatg
    14941 atgttccagg aggaattgat atacatgata gttcagtaat agcagcatat ctagaaggtt
    15001 gtggaataga tggacttcat gtttcacgta gtatacatat acatgatgaa tatatgtggg
    15061 ctcctacgac tttacatgct ggattcagtg cagaattagt tactgaaata aaaaaagctg
    15121 ttagtatacc agtaataaca gttggacgtt atacagagcc acagtttgct gagttaatgg
    15181 tcagacaagg aagatgtgat ttagtagcat tcggtagaca aagcttggct gacccagaaa
    15241 tgcctaataa agctaaaaat ggaaaactag acgaaatgat accatgtatt gcatgcttac
    15301 aaggatgtgt accaaatatg ttccaaggaa aacctatagc ctgtcttgca aatcctatat
    15361 taggacatga agcagagctt aagccagctg aaattagcaa agaagttctt gttgttggtg
    15421 gaggtgttgg aggtatgctt gcagcatggg tatgtgctaa aagaggacat aatgtaactt
    15481 tagttgaaaa atctgaagtt cttggtgggc aaatgcgttt agctgcatat cctccaggga
    15541 aaggagatat tactaactta gttagaagtt atatttcaaa atgtaatcaa tatggtgtaa
    15601 aaatatgtac taatacagaa gctacagtag aacttataaa ggagaaatca cctgatgtag
    15661 ttattatagc tactggagca actccacttg tacttcctat accaggaatc aatgattcag
    15721 gattaattca tgctgtagac cttcttgatg gtaaaaaatc ttgtgggaaa aaagtacttg
    15781 ttgttggagg tggaatggtt ggatgtgaag tggcagcctt ccttggagaa caagaacatg
    15841 aggttactgt tattgagctt agagaagaag ttggtgcaga tgtaatttca gaacaccgta
    15901 aattccttat gaatgatttt gaaaattata atatacatac cattactggt gcaaaagttt
    15961 ctaaatttta tgatgatggt gtcgactatt tacttacaga tggcacagag catgatttaa
    16021 aaggttttga ttcagtagta cttgctatgg gttctcgtaa ttacgaccca ttaagtgaag
    16081 ttataaaaga agttgtaaaa gaaacttata ttgttggtga tgcagtaaaa gctcgtaggg
    16141 cattagatgc aacaaaagaa gcttttgaag tagcattaaa tatataaata ataaataaat
    16201 ttttctttgt aaataactga ataggttatt tgcaaagaaa atgataatat tgggaggaaa
    16261 ttatagttat ggaaatttca ggtagaacag gattatttgc attaataggt acaccagttg
    16321 gtcactctaa atcaccagtg atgtataatt atagttttaa gaaattagat ttagattata
    16381 gatatttagc ctttgatatc acagtagata aggtaaaaga ggcactttta gcaataaaaa
    16441 cttttaatat aaaaggagcc aatgttacta tgccatgtaa atctgctgtt acagaatata
    16501 tggatgaact ttctccagca gctcgtataa ttggagcatg taatacaatt gtaaatgaca
    16561 atggaaagtt agttggtcat ataacagatg gagtaggcta tgttcgtaat ttaaaagaaa
    16621 atgggataga agttaaaggt aaaaagatta caattatggg tgctggtggt gcagcaactg
    16681 ctatacaagt acaatgtgct ttagatggag caagagaaat atctatcttt aatgctaagg
    16741 atgattttta taaaagagca gagcagacag ttgagaatat aaaaaaagat gtaccagaat
    16801 gtgttgttaa tctatatgat ttagaagata caaacaaact ttatgaggag atagaatcaa
    16861 gtgatatatt gacaaatgca actcttattg gtatgaagcc ttatgacaat gaaacaaata
    16921 taaaagatac tagtgtttta agaaaagact tagtagtaac agatgtcgtt tataatccta
    16981 agaaaacaaa aatgattgaa gatgctgaag ctaatggatg taaggctatt ggtggattag
    17041 gaatgttatt ataccaagga gcagaagctt ttaatctata tactggatta gaaatgcctg
    17101 tagaagaagt taatgaatta tgctttaaat agataagagt gatatcttaa acaatgaaaa
    17161 aacatacgta tttcaatatt tttaataata aattacaaat tgtcatagtg ggttttgcat
    17221 catagtccag ttaaatttga agctttaact gactatactt taaatccatt atgaggatga
    17281 gtttattgta cacaaaaatg ttaataaatt aacaagaatg ggagaaaaat tatgaacaat
    17341 aaaaaatatt atcctactgc catagcgttg tatttttcat attttttact tggtattgga
    17401 atttcaattt taggacaata caaaccagaa ttttcatcaa tgtggggggc taaaacacta
    17461 tcagatggaa cattggatgt aagtattgtt ttagctgtta ttgctgcact aggattaggg
    17521 agacttatat cttatccatt tgcaggacct atttcagata agtatggaag aaaagtaagt
    17581 ggtcttatag gaaactttct acatgcaatt ttctttgttg gaattgtatt ttcaccaaat
    17641 ttttatatag catatgtatt tgcaataatt ggaggggcag caaattcatt cttagatacc
    17701 tgtgtaactc cttcttgtat ggagatattt gctagtttag gaacaatagc aaatatgttt
    17761 actaaattta caatagcttt agcacaattt ttgcttcctt ttataattgg atttgttgca
    17821 gcaaattcaa tatcatttaa agttattttt atagtaacag cgattcttat tgtagtagat
    17881 gccattttga tagctgtgtt accatttcca ccagctaata atgttattga taacaaaggc
    17941 aaaacagtta aatcagagaa gatgaaattt acaccaacaa gtatagctct tgtttgtatt
    18001 ggatttacat gtacatctac atttgtatta tggttaaatt gtaatcagga attgggtaaa
    18061 ttgtatggga tggctgaccc tacaaaaatt caatcctttt actcaatggg agtaatatgt
    18121 gcagtattaa taacttcttt acttattaaa aaatatatta aaccaataag aatattagtt
    18181 atatatccta taatagcttt acttatgcta ttaattgtat attttgttca aactccaact
    18241 atttgtatgt tagggggatt tgtaataggt tattcagcag caggaggtgt tcttcagtta
    18301 acaacgtcaa ctgcaaatga aatgtttcca actaataaag gaaaaataac atctatagtt
    18361 atgattgcat ctagtatagc aaactatgtt attttaaata tagcaggtat tattacaaaa
    18421 tcagggggag ttaatggacc aaaatatgtt gtattgttta atgtggcaat aacatttgtt
    18481 ggaattttac ttgcattgtt tgtaaacatg agatatgaaa aagaaaaagt gtatgattat
    18541 gatgtctaat attagtataa tatatgcata gagaagtcta aatctaggat agatttttat
    18601 taaaagtaat taaaattaat aaggatatta tttacagtta taagataggg tatttacata
    18661 attaataata aggatattac tagaatttca atttctagta atattttttt gcgtataaat
    18721 gtaaacttga cgaaataagt agtataatca atataaatta catcatgtaa aggaggataa
    18781 tttggtggag aatgttttta agcgattgca ggaatttaat ggttatgatg gatacaaaga
    18841 atcatttgaa atgaattacc tatgtatata tgaaagtata ccgttaagag aacaagtaga
    18901 acttgcaaat aatctagtag atgaaatttt aaatatgtat aaaagtgaat ctaatgaaat
    18961 ttatttattg gaggatagta attctaagtc tttaatttgt tatttcgaaa tttttatgaa
    19021 aaagataaat actttggtaa aagaaatgat tattgatgag aaatggttat ataaattaac
    19081 taaagaatta atatataaga gtaaaaaagt tgagtatgta aaactagggt tagttttaag
    19141 tgagaaatat ctaaatgtag aaaacttaag agaagttgta gatacatttt ctaaatctgg
    19201 agaatacgta ttttatctga gtaatactat taaaaaactt gaattttaca atacatattt
    19261 atttaattta tctaaaaagg ctacaggttc aataaaagta tttgctattg ttaatatgga
    19321 aaatttagat tctaaaataa attcctatct aattgaagat ggttataagg atacaaagta
    19381 cgaacgatta ttgatgaact atattatttc aattgtagat ttgaatgaat atcttgaaaa
    19441 acgagattta gataaagaaa agataaataa tttagcacgt cttatatgca attacttgct
    19501 aagtgtagaa tttaaatata taggtaataa attagaatta gtgaatcgat ttttacctac
    19561 tgtagtaaat tatgggacta actttgaaag tttatattca attttcctaa tagctataaa
    19621 cgtcttaaaa gatgaaaata tagagtacaa taagattgaa tttgaaaaag aaataaatga
    19681 tatcttgctc agtgaaaaat ggaaaaatat atattttgaa gcattaagag atgctagtgg
    19741 aaaaacagaa gatattataa aaatgtcaga aatttatgat gtaaacttat cttttgatga
    19801 cttgttgcca tatctgaata gagatattag agattttgag gtatattggc atatatctaa
    19861 aaagggaacg acaagttcta gattaaaatt attaaatttt tttgaagaga cgtttaaaat
    19921 agatgattta attggaaaaa tgaaagatat agaaaaagat aaattgactc aagaatatta
    19981 tgatgatatg ttatttttta ttgtgcttaa aggaagtaaa tcactatatc ctgaaggaaa
    20041 gaatatatct ttaaaaggaa tatttggaaa tataaatgaa gtaagaaaag aatccataaa
    20101 tatcttaaaa agatacagag agaaactatc tcttgaagaa ttaaaaattg ttaaagaagc
    20161 gtatgaaaaa gaaaaaaatg ttatattgaa ggatgaatta agaagagtat tatatgaaag
    20221 taataattta aaaaaagaat ttgtaaatat tgaaaaaata aaagtagatg aacatggtaa
    20281 ggatatctat ttgacttcaa tagctgttgc tggttcaagg tttagaaata gagaatatct
    20341 tgaaaaagaa ttggagaaga gtaagatata ctatttaact agagagaaag ataatctata
    20401 tgatgaaaaa gctattaaaa tagttggtga aactggatat gtaataggct atgtcccaag
    20461 aaaagaaaac tatattttga gcaatttatt agatggagga aaactgctgt attgtagagt
    20521 aactgaatat aatttatatg aagattgtat ctacgcaaat gtgtacttga gttataaaga
    20581 tgtaattgaa acagttgaaa actctttaaa gatggtttta gataagagta gaataaaact
    20641 tataaactaa gacgtttata tattgcttag atgatgtaat cctaactgat tggttatttt
    20701 ttctggaatt atggtgtatt ttatgagcaa tgttaatgaa tataaaataa aaaaacaatt
    20761 gttttgtata tatgatgtgt ataaatatat ttatatattc atatttacaa aacaattgaa
    20821 atttatgtta ttttatgatt ttaaatttag atggaatttt aacttctgct ttatctttta
    20881 ttttagaagc ttgtttttta actttattta ataattcaga ttcatctaca actaatactt
    20941 ttctttcctt catcaaccat ttaccatcac aaatagtaga atctacattt tcactagtca
    21001 tagaataaac tatatttcca attggattat gcattggtaa acaatgaatt gtatctggat
    21061 ttagaataat catatcagct ttttttccta cttcaagact accaatttct ttttctagta
    21121 aagcacattt tgctccattt atagtagcca tttctaagac ttcttcagct ggtacgacag
    21181 ttgggtttaa agttctacct ttatgtatta gtgatgtaag atacatatcc ctcataaggt
    21241 ccattctatt atttgaagga gctccatcag taccaattga aacaggtatt cctttttcta
    21301 acatttcagg aattgaggca aatcctagaa ctactttcat agctgcacct ggattatgag
    21361 aaacttttac atcatgtaat ctaaataaat caatttcttt atgagtaagc caaactgtgt
    21421 gagcagctaa aagatttggt cctaatacac ctagtttatc aagatattca actgttccaa
    21481 ctccgttgtt ttgctttaca aatgaaattt catcagctat ttcagcaata tgcatatgta
    21541 tacctgtatt gtatttatca gctaactctt tagtcctaat aagcaattct tcagttgtat
    21601 taaaaatagt tcttaatgca aaccatattt taatcctctc attagatgtg ttattaaact
    21661 tatggaataa gtctaattga gtgttgattt cctcatcagt agttttttgc catgcttcag
    21721 gaagaccatc tcctgtatcc ataactgatt tagctaaaca ggctctaagt ccagttttat
    21781 caacagcttc aaccatagca tctacatatt gaccaccaga ttctaaaaat gtagttaccc
    21841 cagattttat caattcaaca caacatgcag tagatgaaat caatgaatct tcatagttga
    21901 agctactttc gtatggccag attctctctc taagccaagt taaaagtaca acatcatctg
    21961 caagaccacg tcctaattgt tgggataagt gaacgtgtgt attgactaac cctggcatta
    22021 gtatttttcc tttagcatca tatatctcag catctttatc tacaatatta atatcaaact
    22081 taccaacatt ttttataata ttattttcaa taagtatact tccagtttca tatacctctc
    22141 tatctgcatt catagtaatt agatatgcat tttttataag taattgtgac ataagtacaa
    22201 cctcttttct atatattttt atatctatgt attatgataa taaattttct atacaaagtt
    22261 aatagcaaaa tcaatttata cataaattga ttttgctatt tataaattta aaatattgac
    22321 ctagaactat ttaaaatatt aacttagaac taagtttaca actgcaagta aacctataat
    22381 ccaaagtgta atactgattt tatcagtttc tttagctacc acatgcatta ttatatatga
    22441 aataaatcca aagcataaac caatacttat actataagtt aaaggcatca ataaaactgt
    22501 aacaaaagca ggtacagcta ttttgctatc agcaaaatca atctgtttta tgctttcaaa
    22561 catgaataca ccaaccatta caagtgcagg agctgcagcg taagtaggaa ctataccaag
    22621 caatggtgta aatataagtg tagctaagaa taatgctgct gttactattg aagcaagtcc
    22681 tgttcttgca cctgctgcga taccagaagc agattctcca aatgttgtaa cagtactagt
    22741 tcctaagaat gagcctatta aagtagaaga aacatctgca tataacattc ttcctaaatc
    22801 ttttatttca ccattctcat cttcaagacc catttgttta caacaagtaa taagaaatgc
    22861 taatgaatca aacatgtcta taaacatgaa agagaatata ggtccaatta atgagaattt
    22921 caatgcacct acaatatcta atttaaaagc aactggtgct atactaggag gtgttgagat
    22981 tattgctgtt ggcaactcca caaatccaaa tataacagat attattgttg caactactat
    23041 acttatcaat attccacctt taactttttt atattctagt attgccatta agataagagc
    23101 aaaaagagct attaaaactg ttggtgtaaa atctgcaagt gaaactatag tatctggatt
    23161 agctattata acacccatat tttttaaacc aacaaaagca aggaaaagac ctatacctgc
    23221 tgaagatgct actgccaatt ctctaggaat tgcgtctgca attgtctttc gaagacctgt
    23281 tactgcgagt gtaaagaaga cacatccaga tagaaaaact accccaagac ctgtttgcca
    23341 tgggattcct gcacctataa ccaaagagta tgtgaaaaat gcatttaatc ccataccagg
    23401 agctagagca aatggtgcat ttcctaaaat cgcaaatata agtgtaccaa tggctgtagc
    23461 tagtatggta actgttataa gggcggcttt atccattcca gcttcagata atattgctgg
    23521 atttacgaaa actatgtatg ccattgttaa gaatgtagta actccaccta ttacttctgt
    23581 tttaaggcta gagccacgtt cgctaaattt aaacattttg tctaaaaagc ttaatgatga
    23641 attatttcca tctggttgta cattgttagt gtttggtacc atttttacct ccaaaaaact
    23701 ttaagatata aacaaaaaaa tatatattca tatagataga atagaacata tattcaaagt
    23761 tgatattcta ttcatagaaa acccatttaa ggtgagtttg tagaaactat tagaccgtat
    23821 tactaatatt atatgaacat tttctatttg tttttctgtt gtattatatc gtaatattgt
    23881 attatttaca ataaatataa taaaattatt cgtttttttg tcgattttat aaaaaattaa
    23941 ggttatttat tggcaagaat tactgttttc aataattgaa tatgtataag tataatgtca
    24001 attattctaa cttgttttat actattatat agaagtctat tataaatgag tttgtgatat
    24061 aattataaaa gtactgaata cacggatatt tcgtaaaaac tttttaatat atcaaatatg
    24121 tatattaagt taatatatta aaaaaaataa tatcaaaaat aatataattt atagtggagg
    24181 ggaaaatgac taaaattatt aaagtgcaaa aacctaaatt tacaggagaa ttagaaatta
    24241 ttgaaaatct tgaagatata cttgaagata tatttaatga tgaaaaaata tttaataaaa
    24301 ttatagaaaa tgaagttata agtgatttag attctgtaag agttagcttt gaatcttgta
    24361 tatttagaaa tgtatccttt gaaaattgca atttaaaaaa gattgattta cttgatgtgg
    24421 tatttgaaaa ctgcaattta tcaaattcat tttttgatgg aggaagtata tacagagttg
    24481 aatttaaaaa tagtaaattg ataggagtta gatttgatga ttgtatactt aaaaacgttt
    24541 tgttcaagga tgttttaggt aagtattcta acttttcatt ctctaaattt aagatagttt
    24601 ctatcttaga ttcaaatttt gaaaattctg tatttcaaga agtaaaaggt gacaatctag
    24661 tatttgagta tacaaactta aaaaaagcaa actttaatgg aactaaactt tctggcatag
    24721 attttaccac taataatatt gagggaattg agattggtat agatgagatt agaggagcaa
    24781 tttttgatgt gtcacaagca attgatttaa cgaaattgat gggaattatc ataaaatgaa
    24841 aaaaatattc ataaaataaa ttgggaaaac gttaacaatg tgtgaatttt taccagattg
    24901 acatttttct gaaaataaag taagattatc ttaatattca aaatattttc aaagggggta
    24961 atttagtgtg agtaagcaga tgagagatat ttttatagtt ggttttgctt tattttctat
    25021 gttctttggt gcaggaaatc ttatgtttcc tccattttta gggatggaat ccggcaaaga
    25081 ctggttaatt cctcttattg gatttgttgt tgcagatgct ggtatagcaa tgcttgttat
    25141 tatagcaaca gcaagatgca agggaagcat ggatgatgta cttaggagag ctggtaaagg
    25201 gttagcaaga gtaatgagtg tagctgctct agcatgttta gcacttcttg taataccaag
    25261 aacttgtgca acaacatatg aaatgggtat tatgcctata tttggagacc atggaccagt
    25321 agctaaagct atattttcaa taatcttctt tgggttaact ttagcattta ctattaggtc
    25381 ttctaaggtt atagacataa taggtaagta tttaactcca gcgttattat tagtattatt
    25441 tgttttaata gtaaagggta taatttctcc agcaggacct atgagtcctg agcatatgat
    25501 agataaaaat ctatttggtg aaggtatatc acaaggttat caaacaatgg acgctctagg
    25561 tgcagcatct atggcaacta taatgttgat gtctataata gctaaaggat atacatcaga
    25621 taaagaccaa ataggaatga ctattaaagc tggttttgta gcctgtgtat ttctagcagt
    25681 agtatatggt ggtcttactt atttaggtgc aacagtttca actttatatg atactagtat
    25741 acctcaagca tctttattag ttgaaataac tagtatattg ttatatggtc aagtaggaaa
    25801 ggtaatttta ggtgtaattg tggcacttgc ctgtctgact acttctacag gtttaacagc
    25861 atcaatatca acttattttg aaggaataag taaggcgagt tataaacaac tggttatagg
    25921 tatatgtgta gctagtatgc ttatatcaaa tttaggtgtt gattctataa tagctatatc
    25981 agttccaatc ctacaaacta tatatcctgt tttattagca atagttgtta tggagttggc
    26041 tggtaaacat ataaagaatg ataatgcttt taaaggtgca gcatatgtta cattagtgat
    26101 tagtctatta tcagctataa atggaatgac aggagctgtt ccatttatac aaagcttgcc
    26161 actcgcaggt ttgggcttta actgggtaat accagctata ttgggtggaa tagttggtaa
    26221 ctttataaaa agcaacaaac aagtagcata ataatttgta attaatttaa ttttataata
    26281 tattaatttt tagtactatg ggtaggttta atctaaacct aatctaccca tagtactttt
    26341 tagattggat tggaatagca tgtatataat tttttataat ataatttttc tttgaagtat
    26401 gataaaatta gtaatagtga taagaaagta caaatttagg aggttttaat taatgcttag
    26461 aaaaataaca gcatttatgc tatccatatg tttaattatt ggacttacag gttgtagttt
    26521 acttgtaggc aagaaagata aagagaaaat tatagatgat tttaaagttg cagtggtaac
    26581 tcaaccatta tctgaaaata aagtacaata caatatggtg gaagagatga caaaagaata
    26641 tgaggaagag aataaaattg ataaagacaa agatggtcaa actaaagtta aacaaactat
    26701 aaaacatgtt gtattgccag agaattttac ttctaatata gatagtgcta ttaataagat
    26761 agtaaagttg gctgacgata aagaagttca ggctattgta gtttcaactg accaagcagg
    26821 gcttttacct gctttacaaa aggtaaagga aaagcgtcca gaaataataa ctatttctgc
    26881 ccctatggga gatgataaaa accaattaag ccaatttgtt gatgtgaatc taggggttag
    26941 tgcagaggaa agaggaaaag tattagctga acgttcaaaa gaaatgggag caaaagcttt
    27001 tattcattat gcctctacag atgatttgaa agatgttaat atagccaaaa gattagaaat
    27061 gattaaagag acttgtaaaa atataggatt gccatttgta caagttaata ctccaaatat
    27121 aaatactgaa gaagacaaaa acaaagttaa gcagttttta aatgaagata ttgaaaaaca
    27181 agttaaaaaa tatggaaaag atataaatgt atttggtgta aatgagtata tggatgaagt
    27241 tatattgact aaggctttag aattaaagta tatagttgca gagcaaagca atccatcacc
    27301 aatacaaacg tatcctagtg ttatgggtct aaaaatatct gaaaaagatg ctcaaaatta
    27361 tgataaaata aacgacatga taagtgaaaa ggctaaggct tttggtatgt caaatagact
    27421 tggtggatat cctatgccaa tggatgcttt tctaccatca gtagcaattt atcttgctac
    27481 tgaaatggtg aaacaagatt taacacaaga agatgtatgt gacccagatt atttagaggc
    27541 gtttacagaa ttaagatttg gaatcggttc agaatttact cctttaacag aggtacttta
    27601 taattatcaa tcagtaattt taagccaatt aatttattaa tattaaggaa gtgtctcaaa
    27661 atgaactttt tagtttatga ggcactattt ttctgttaaa tcaaatatat ttttatcttt
    27721 ttaacaatta aaaagaagct tatccctatg ttgagatagc ttctttttta atataaataa
    27781 gttttattat tttataaaaa atttatagaa aaataaaaaa ttataattat atataaattt
    27841 tgataaagta aagatgaatt actataggtt taaattatat tattttctat tatttttatg
    27901 cctttctcag ttattgatga gccttttctt cctttactag aaattaagta tccattgcgt
    27961 gtcaaatatt ctagcatatt tcttacttcg tattctgtga cattatattc cataaatttc
    28021 attaactttg tcctccccat tggtttcatg tgcaatgatt ttaggataga aatgcatatg
    28081 tttttgtcat attggtttaa atcattgaat atagacatat tacttgacat atgattatta
    28141 tttaattttg aagatatgtt ttgaggtaaa tcatttattg taagataatt ggaacccatg
    28201 taagccatat attcaacaca atttctcagt tctctaatat tccccatcca agtgtatgaa
    28261 tttaaagctt ctaaaagact cttatcgagt tttatgtttt tatagggaag ttcttttaaa
    28321 aatgattcta aaagaagagg tatatcttct aatctctttc taagaggagg taaatctaaa
    28381 gtgaacatac ttatcctata aaataaatcc atcctaaatt tattttctat aattaatttt
    28441 tctaaatctt tatttgtggc agctaatatt ctcacatcaa ttggaataat acttgtacca
    28501 ccaatttttc taatttcttt ttcttgtaag actcttaaaa gtttaacctg taaatcatgt
    28561 ggcatatctc ctatctcatc taaaaatata gtgccagtat gagctaattc gaataatcct
    28621 ttttttccac ctttttttgc tccagtaaaa gaaccatctt catatccaaa tagttcactt
    28681 tcaagtaatt cacttggtaa tgaagcacaa tttattgcta caaaaggttt attttttcta
    28741 tttgaatgat tgtgtattga ctgagtaaag gcttctttac ctgttcctgt ttcacctgtt
    28801 attaatatag gattatctgt aagagccatt ttttttgccc ttttaataca atcttttata
    28861 atagaactat ttccaataat attattaaat ttatatttag ctacataacc tcttttgaca
    28921 gaatcacttc ttattttttg ttctaatatt tctatattgt ttacatcttt taatgtaatt
    28981 aagcaaccct ctatattttt ataaagataa aatggttttt tagaaagtat gaatttttta
    29041 tctatttcgt ctatgtatat aatcatatta tcgatattta agttctttgt aattttatct
    29101 aagaaactct ttggcatata ttccattaag gaaggtgatt ttataagttt ttcatctagt
    29161 tccaacatat taagtaaaga cttatttaca aaagtaacct tgttaaaggt gttgaaaaat
    29221 attagaccat cacctatttc atcaatggtc atgtaaagta tagtttttaa tttagatata
    29281 ttatctaatg acgtatttaa gaaatcatga cttagtgtct ccttggacaa tttgtatagt
    29341 ttttctataa atttctcatt ttttagtttt aatactacta ataatgacat atatgtatct
    29401 aatgagattt ttctccaacc taaatcaaca atatttttta tgtattttgg tataaatcta
    29461 atctgacctg gtgttatagc tgtatctaaa tcatatattt catcacaatc tggatatgtc
    29521 ggtacaaaat ctatatgatt tataccaatt ttatatagat aagtagccat ctcaaatgct
    29581 gaagttttat tggttgtcat aagtaacgat tttgtacctg gaggtagttt catcattttt
    29641 tcatatccaa gttttgaaag tgctttcgtt ccatgaataa tttcaatatc ttccttaaaa
    29701 tgttttatta aaaaattact agttaaaggt gatagagaaa gcactacctc tccctctaaa
    29761 ttttctttta ttccatcaag tatattttgt gtttctatga ttatttcgtc tccaaaaaag
    29821 tttgttaatt cttctttgta gtggtcacta tttaatttat catatgttat aaggattaac
    29881 ttctttttat ccattttatt tctcctaaca aaatagtatt tatttttaaa gtatttaaag
    29941 tataaggtaa gttatattat acaacaaata gtagtataaa tttatatatt tgattaaata
    30001 ataggttttt ttaggatttt atcctataaa aacctatttt aaaattattt ttgcaagata
    30061 aggaaaatag ctttttatat tgtaatgtat attttaaata gttaataaaa aaatattata
    30121 tttttaaact tgaaaagtaa gtaattataa gctatttaat cgctggaata aatttacaat
    30181 aaaaaaataa atacaaaggc tttatcatta aggtattggc ataagttttg cttattaaac
    30241 aagtatacta ataaaattca tttaaacagg agggagaatt tatatgagta aattagaatc
    30301 aacaacagaa aaagttgagg taaaatggta tgggtatgta gcattaatat tgggagcatt
    30361 atttttttca ggaatattta aagatgcacc aggaccatta aaagtattgg attttaacaa
    30421 tgtattaggt tcttttggaa cattgggaac aattaatgat ggtgtaggta cattagcttc
    30481 aaactttaga ggtgatggag gtactggccc tcgagatgga tggctatatg cattgacatt
    30541 aataccatca gttatgtttg cacttggagt agttagagtt atagaccact tagatggaat
    30601 gaaggcagct caaaagcttc ttagtccact attaaagcca ttattagggt tacctggatt
    30661 tgcaggacta actttgatag ctagtttaca gtctacagat gcagctgcat ctatgactaa
    30721 agaacttaaa gatgatgggt atatagatga aaaacaaaaa gctgtatttt gtgcatttca
    30781 attttcaggt gcgtctgcaa ttacgaattt ctttgcttct ggagcagcac tatttccatt
    30841 tataggagat gtaccaatat ttataccact tgctttaata ttaattatga agtttgttgg
    30901 ggcgaactta cttagattat atttaaataa atttgacaag ggggaagctt aacatgagtg
    30961 aggtagcaaa aaaagaagta aaagttaaaa aaggaaatgt gatagatgcg tttattggag
    31021 gagctagaaa tggattccaa ataagtacaa attcaatggc tccaaatgtc atcttcggtt
    31081 ttgctataat ttctgtgttc aatttaacag gtcttttgga tgtaataggg acaatattta
    31141 cacctgtaat gagtatcttt ggacttccag gtgttgcagc aacagctata atgacaatat
    31201 ttatatcaat gggaggagca tctggagtaa tcgcaggttt atttacagca ggtcaaataa
    31261 gtcaagcaca tgttgcaatt ctattgccaa tgttattgct ttcaggagcg cagatacaat
    31321 ttatggggag acttttggga acagctgagt taaaaactaa atattatcca catatgtttg
    31381 ttatagcaac tttaaatggt tgtattgcaa tgcttataat gaattatttc gtagtatagg
    31441 taattataat aaaaatttaa tataaatgga aagtttaaat tttaaggagg agctagatta
    31501 tgggaactaa caaagattat attgagtatg tacataaagt gtatgattat ctacacacta
    31561 taccagaaaa aggatttgaa gaatataaga catcagagtt tgttttaaaa gagcttgaaa
    31621 aaatggggtt tgaaaatatt aataaaggag ttgcaggaac tggtattata gtaactatag
    31681 attctaaaaa agaaggacca gtatttgcta taagagcaga tatggatgca ttggaattta
    31741 tcatagatgg agaaaagaaa atgatacatg cttgtggtca tgatggtcat acttctatgc
    31801 ttttaacagc tgcaaagtta ataaaagaga gaaatttagt aaaaagaggt aagttaaaaa
    31861 tactttttca accaggtgaa gaaaaattat atggagcatt aagagtaatt gattctggtt
    31921 tgttggatga tgtagaagaa atggtaggga tgcatttaag acctggacaa gattgtaggt
    31981 tgggaaaggc gatacctgca atgtaccatt gtgctagctg tataatgcaa tttacgatta
    32041 aaggtatcac ttgtcatggt tctaaaccac atttaggagt aaatgcagta gatgctgtct
    32101 gtttagcagt gaatgcaata aatgcaataa gagtaaatcc agctatacca aattctataa
    32161 aagtaacaaa tatatcagtt aagggagaaa cttataataa tataccagat gaagcattta
    32221 tggctttaga tataagggca gaaagtaatg atataataga agaattaaaa gaaaaagcaa
    32281 tacatgctgt aaaaactgct gtaagttcta taaatgcaga agctgtacta atttccaaag
    32341 atggagtacc agcagcagaa tttgatgaag aacttgtaaa cttggctgaa gaaactataa
    32401 aagatgtatt gggagctgat gcatctatag gtatatttaa aaattctggt ggagaagatt
    32461 tccattatta tacacaaaaa ctaaaatgta aaactacata tattggatta ggagctgatg
    32521 ctacaccagg atttcataat ccaaatgtaa catttgatac gaaagcttta gaatatggtg
    32581 tagatatatg gtgtagattg gttgaaaaga gattgggata atatccaaac taataattct
    32641 acatttaaaa ttataattat agaagttata gaaaaagatt gcttatgtta attagaaaat
    32701 aattaatatg agcaatctta ttttgatata atgaaactga ttttggaata ttgaaaaaat
    32761 atgatggaaa agtaattata ttttaaatct attggacaaa aaagtgatat tattataata
    32821 ttaagaattg taaaataata ccaaagtgca tctagtaatg gacttaacaa ttataaaaat
    32881 tttattctta ggagggaaaa aatgagtaag tataattttg acagagtaat agatagagta
    32941 ggaacagatt gtgtaaaatg ggattttaga actaattgtt caacaaaagc acaaaaagat
    33001 ggtttgcctt tttggattgc tgatatggat tttgaatgtg cagaaccaat aatagaagca
    33061 ttacgtaaaa aagttgaaca taaaatattt ggatatagtt caaatgatag tgataaatac
    33121 tttaatgcag tttgtgattg gtataaaagg agatttaact gggaaataaa tagaaaagat
    33181 attattttta gtcctggtgt tgtacctgct gttgctattt tagtaagaat attaactaat
    33241 tctaatgaag gtgtaatagt ccaaaaacca gtgtattacc catttgaagc taagataaag
    33301 agcaataata gggacattgt aaacaatcct ctaatatatg agaatggtac ttataggatg
    33361 gattatgatg atttagaaga aaaagctaag tgtagcaaca ataaagtact gatactttgt
    33421 agtcctcaca accctgttgg aagagtttgg agagaagatg aattaaagaa ggttgtagaa
    33481 atatgtaaaa aatatgacct atggataata gctgatgaga tacattctga tttagttaga
    33541 aaaggattta aacatactcc tttacagtca ttatgcccag agtacaagga taaaataatt
    33601 acatgtatag ctccaagtaa aacatttaat ctggcaggaa tgcagttgtc taatataata
    33661 gtgaataatg atgaactaaa aaagaaatat caagaagagg ttacagctgt tggagttgca
    33721 acttcaccaa atccctttgc tattgtagct acaatagcag catataatga aagtgaagac
    33781 tggcttaatg aattaaatga ttacttagat aataatatac aatttattga tgaatactta
    33841 aaagaaaacc taccaaaagt aaaacttgtt tatccagaag gaacatatct agcatggtta
    33901 gattttactg cttatggatt aaatgaagta gaacttgagg atttaatgtt taaaaaagct
    33961 aatgtattat ttgatgaagg ttatatattt ggaaaagaag gaataggatt tgaaagaata
    34021 aatgtagctt gtccacagtc tttattaaaa gaatgtatgg acagattaaa gactatattt
    34081 gaaaatttat aaagtatagg taaaaattta taaagtgttt aaaaataaaa aaataataaa
    34141 cttgacaaat tttaactaat cacttatcat actattatga ataaatagaa aacataagag
    34201 agctaaattt tattgaatta aactcttaaa gactttgaaa ggaagagtag ttatagaatg
    34261 taattaaagc gagttggtga tggtgtgaga ccaatatgaa gtctataatg aagaacatcc
    34321 tggagttgct aaccgaaatt ttaaaagagt aggcttagtc ggaaccaaac cgttatcata
    34381 ttggggtgta tcgagtaata tctgtacatt gtgagctggg tttgaattta tttcaaacta
    34441 atcagggtgg taccgcggaa aagcaacctt tcgtcccttt tatataggga tgaagggttt
    34501 ttttaatgga aaaatatata aatataggga gtgaatgata tgagcacaat aataccaaag
    34561 gattataaaa gtagtttgaa cgtaattgat acacaaatag ctataaaaaa attaaaagat
    34621 ttttttgaaa tgagactaag taatgaatta aatcttataa gagtttcttc tccgttattt
    34681 gtattgccag aaacaggtgc aaatgataat ttaaatggag aaaaggctgt aagctttgat
    34741 ataccattta tgaataaaaa tgcagaaatt gttcaatcac ttgcaaaatg gaaaagatta
    34801 gcccttaaaa aatatgggtt tgaagttggt agtggtttat atacagatat gaatgcaata
    34861 agaaaaaatg aagaattaga caatatacat tctctttatg tagaccaatg ggattgggaa
    34921 ttggttatag ataaaagtag tagaaatgaa aagactctaa aagatgttgt aaaaagattg
    34981 tatggagtat ttaaagatac tgaaattttt gtatgtagta tgtatgaagg tataaaagaa
    35041 atattaccag aagaaataac ttttataaca tcacaagagt tagaagatat gtatccagaa
    35101 ttatcaccga aggaaagaga agataaaatt gtaaaagaga agaaagcagt atttataact
    35161 caaataggaa aaacattaat atcaggagaa aaacatgatg gaagaagccc tgattatgat
    35221 gattgggaat tgaatgggga tatattattt tacaatcctg ttttagattc agcattagaa
    35281 ttatcttcaa tgggaattag agttgatgaa gaatctcttg atagacaatt gaaacttgct
    35341 ggatgtgaag agagaaaaga gtttgactat cataagatgc ttttaaatgg cgaacttccg
    35401 tatacaattg gtggaggaat tggtcagtca agaatatgta tgtatttctt acaaaaagct
    35461 catataggag aagtacaagt tggagtatgg ccacaggata tgattcaaaa ttgtagtagt
    35521 gcaggaattg aattattata atttaaatat aaactaattg ataaaattta tatctaatat
    35581 gttcatcaaa ttaattatat cggttataat gtagaataac ctataattat aagaggtgat
    35641 aaatatggag tttttaaaaa taatcattaa tatagtttta gatattttaa aaaaaatttt
    35701 ggtcagattt aaaaatgcta aatttggttt ggtttttgta tttgatttac ttaaactacc
    35761 tgattttatg acagataaaa gaatcaatat agttgataaa ataaaggtaa tttctgtatt
    35821 aatttttact atatcctact ttgtatctgg ggttgatata attccagaga tgattgcagg
    35881 ggcatttggt tttatagatg atgctatagt tttaatttgg agtataggta tagtgaatga
    35941 agaaattaat aagtatagag ttattataaa aaaagataaa cattcaaata taattgaaaa
    36001 tgtagaattt tctataaaag atgaagaaga ataacaattt atatatgtta ttcttttttt
    36061 tattaacaaa aaatggttaa attgttgacg gagaggtaaa actctattat cataataagt
    36121 ggatatccaa tatttaacta agtattcaat gtgcaagaat gcttaattta aaaaagttat
    36181 ggagggtaaa tattatgaag aaaaaaatag gtttagtgcc caaactaatc atcggtatca
    36241 tagtcggtat tttaatcgga agttatgcac cagaaataat agtacaaata ttagtaacag
    36301 ttagtacctt attctcagca ttcctaaaat ttgttatacc atttataatt ataggttttg
    36361 taacagcagg tatagctgat ttagcaacag gtgcaggaaa gttattgggg ataactacgg
    36421 gaatagcata tggttctacc ttaatagcag gattattatc atttgcagta tctacattaa
    36481 tcttcccaaa ttttattgat gcaagtgttg caagtcaaat tggagaccca gaggcaggaa
    36541 tgttagctcc aatattcaca attccattag agcctatggt agatgtaaca gctgcaatcg
    36601 tatttgcatt tgtaatggga cttggaatat cagctttaag aaatcatgga aaaggagaga
    36661 ctttatttaa tttcttccaa gaatttcaag gaatcgtaac gaaaacgttg tcaacagtga
    36721 ttataccttt attaccttta tacatagcag gaacttttgc aaatataact atagcagggg
    36781 aagtatggac aatattaggg gtattctgga aagtatactt agtagtaata cctttacatt
    36841 ttgtatatat ggcatgtcaa ttcacagcag caggtgtatt ttcaggaaaa aatccagtaa
    36901 gaatgttaaa aaatcaagta cctggatatt taacagctat aggaactcaa tcttcagcag
    36961 caactatacc agttaatgtt ggttgtgcag aaaaaaatgg agtaagtaag caaataagag
    37021 aatttgttgt acctctttgt gcaacaatac atttatcagg tagtatgata tcaataacag
    37081 gatttacaac tgcagtttta atgatgaatg gtatgccaca tggaataggt gtattattcc
    37141 cttatattgc aatgttaggt atagcaatgg ttgcagcacc aggagcacca ggtggggcta
    37201 taatgagtgc acttcctttc ctaccaatgg ttggaatacc atcagatggt gggcttgcaa
    37261 gtttaatgat agctttatac ttaactcaag acagttttgg tacagcatgt aatgtatcag
    37321 gagataatgc aatagcagta gtagtagata aaataaatga tggaatgagt aaaaaaggaa
    37381 acaaagaaga gcaaagtgct tagtttttta ataatcaaaa atgagttatc tcaaatagag
    37441 atagctcatt ttttatggta taaaaaaatt ataaaataaa gggatttttt tatttaaaaa
    37501 ttgctttaag agatgatatt aataatttat tttcattagg ttttaatata catactctaa
    37561 agaaatactc atctagacct tcaaatgaac aacaatctct tattattatt ttttgtggaa
    37621 gcagttgttc tcgaagcgat ttagcagtta attcctttgt tttgatttta cacaatatga
    37681 aattaccttt agtattgtat atatctaagc tttttatatt cttaagctct ttaagcaggt
    37741 aatctctttc agtgttcatt aaggaaatag tatttgatat aaaatctaaa tcactaaaca
    37801 tgatttcccc cattttagaa gctattatgt ttatattcca taaatctaga tttttattta
    37861 tttcattttt gacattagtg ttagatataa gaccatatcc aagccttatt ccaggcgttg
    37921 aaaaaaattt tgatgtacct cttataacga ataaattaga ataatcatca actaattgag
    37981 tacaagagta tgtatctgta tcagtaaact ctacataagt ctcatcaatc attaaaaatg
    38041 agtctgtatt ttttaaaatt tctctcactt ctactttagt aaatgcaaat ccagttggat
    38101 tactaggatt acagattact accaaatcat aatctttagc attgatagtt tttataagat
    38161 tttctaaatt tatatgaaaa ttatcttctt cttttgcaaa gtatttttct atagaacaat
    38221 taattttaga taactctttt tcatactctg aataggctgg agagagtaaa agagcctgtt
    38281 taggattaat tgtatgtata aatgatgaaa ttagttctgt agctccactg ccaagtacta
    38341 tattatctat agaacatttg cagtaattgg atatagaagt ttttaagtct atgtaatcag
    38401 ggtcaggata catagatacc atatctatat tgtttacaat gtattgtttg gctaaacttg
    38461 atgtaccaaa aggatttata tttgagctaa aatccataaa ttcttccttt gaaaagccat
    38521 acttactaga taaactatat aaattagctc catgatttgt gctcataaaa gtacctccaa
    38581 aaataaaatt atataatcga taaaaataac tgattaatat atgtacatat agattataat
    38641 acaaaggaat cgttattcca aatttttaat ggaaaaagca aaaaagtatg gataagctgt
    38701 taatttaggt ctaaaaatga tgaaaaagat tttaattgat attttggtat gctataatat
    38761 ataagttaat agaaggaaag cgggaggata caaaatgtac aaagttacag gaaaaatgtt
    38821 gacagaagaa caaataaaag aaaaagttta tgaattagga aaaaagatag aagaagactt
    38881 taaaggcgaa gatttactag tggtaggtat acttaaagga gctagtgtat ttgtttcaga
    38941 tttaataaga tgtatagatt tggatgtaaa tatagatttt atgagtgtta caagttatgg
    39001 aaactcaaca gagtcttctg gaacagttaa aatattaaaa gatttagatg tagatattga
    39061 aggaaagaat gtgttgattg tagaggatat aatagattct ggtttaacat taagtaatct
    39121 agttgcagct ttaaaaacta gaaatccaaa atctttaaaa ctatgtactc tacttgacaa
    39181 acctcagaga agaaaagcta atatacctgt agactatgtt ggatttgtta tagaagacaa
    39241 atttattgtt ggttatggaa tagactatgc tgaaaagtat agaaatttac cttatatagg
    39301 tatagttgag gatgtagaat agatttaaat gtgaatcaaa agcactaatt tctaagggag
    39361 ttagtgcttt ttagttaatt atataaaatt ttagtaaagg agacatctat atggatattc
    39421 agttaaatat attattattt ttccaaaatt taagaaatcc aattttgaac tttatatttc
    39481 ttgtctttac aataagcaca gaagcaccgt tattaatatt aattacaaca attatatact
    39541 ggtgtataaa taaaaaatgt ggacagaaaa tactgtttgc tataattgga aattttgttg
    39601 taaatttagg catcaaagag tttgtaaaag ctcccagacc aataggaata aaaggtcttg
    39661 aatcattgag agtttctact gctggtggtt attcttttcc aagtgctcat actcaaactg
    39721 caacaacatt ttgggttagt attatgacta tatttaaaaa gagatatctt catttaatag
    39781 gtgcagttat ggttttagga gtaggattgt ctaggctgta tttagctgtt cattggccaa
    39841 tagatgtgat agtgggttgg atgttaggaa tattttttac agttatattt ataaaaatat
    39901 ttgactatat tgatgataat aaaaattata ttttacttgt agttttactt ataccattta
    39961 taatagtagc tatatttcta aatagccctg aatattttga aaaatttggt ataatagtag
    40021 gatttgtatt tggatatatg gttgaggata gatttgttaa atttaataca gataataata
    40081 acaaaaaaat aaattttagc aataaaaata aggggagtaa aaatataatt ttttcaagaa
    40141 tatgtaggtt tttggttggg atagtaacaa taggaatgtt atatattggc attaagttgt
    40201 ttatttcaat gttaatagtc actttaaata taagtgactc tactatgagt ataattttta
    40261 tgaattttat taagaataca gtagttgttt tttatggaat agcaggagct ccagcattat
    40321 ttaaactttt aaaattaaat tgattttaag gtatagagtc aatagtttaa attaaaaaaa
    40381 ttaagggtag aaaagagtga ttatttgaag aaggtagtaa taataggtgg tggaccagca
    40441 gggatgatag ccgcatcaac tgcatgtgag aaaggctatg atgtaacatt aattgagaaa
    40501 aatcataaat taggaaaaaa acttgcaata acaggaaaag gtagatgtaa tattactaac
    40561 gcttgtgaaa tagaggaatt aatagaaaat gtacctacaa atggaaaatt tctttatagt
    40621 gctttttata cttttacaaa tgatgatgta ataagtatgt ttaataatct tggagtaaag
    40681 actaaaacag aaagaggaaa aagggttttt ccagagagtg ataaagcctt tgatattgta
    40741 aatgctcttg aaagacaact taaaagtaag aaagtaaata tattattaaa ttcaaaagta
    40801 gaaaaaataa tatctaaaaa taataaaatt gagaaagtaa ttttaaacga taaaaaagaa
    40861 attaagtgtg attcagttgt agtagcaact ggtggattaa gttatccact tactggttct
    40921 actggtgatg gatataaatt tgcaatttca caggggcaca caattataga tacaaaacca
    40981 tctttaattg gtatagaagt acaagaaagt tttacaaaag atttagaaaa attatcattg
    41041 agaaatgttg aaataagggt gtttaatagc aaacaaaaga aagtttatag tgattttgga
    41101 gaacttgaat ttactagatt tggtttagat ggacctataa tcaagtctgc aagttgtaga
    41161 atgaaagata caaggaaaga aaattacact atattattag atttaaaacc tgcattagat
    41221 gaggaaaaac tggataaaag agttcaaaaa gattttcaga agtatacaaa taaaaaattt
    41281 gaaaaagcat tagatgattt acttccaaaa aaactaatac ctataataat aaacttaagt
    41341 gaaattaatg caaatacggt tgttcaccaa atttctagag agcagagaaa aaacttagtc
    41401 catttattaa aaaatttaaa gtttacagta aagagataca gacctattga agaagctata
    41461 ataacttctg gtggtgtaaa agtaaacgaa ataaattcaa gcacaatgga atcaaaatta
    41521 gtagaaggtt tattttttgc tggagaagta atagatatag atgcatatac aggtggattc
    41581 aatttgcaaa tagctttttc tactggatat ttggcaggtt ttaattgcta aaaattacct
    41641 gaattaaaaa tgtttaaaat cacataatat tatcagcaac acaaaataaa ttaaacagaa
    41701 ggaaggtaat caacatggca agtaacaata acaacaacag aacagtagta ccaggagcaa
    41761 aagaggcttt aaaccaaatg aaattagaaa tagctaatga acttggtatg agtaattatc
    41821 aacaagttga taaaggaaac cttactgcaa gagaaaatgg ttatgtaggt ggatacatga
    41881 ctaaaaaatt agttgaaatg gctgaacaac aaatggctgg aaaaagcaac agatagttat
    41941 aaataattat ttataactat tggctattag tttagctaga gctatctcaa atatagaaag
    42001 taatttctaa attgagatag ctttttttta ttaaattatt aaatagatat atttttatgt
    42061 aataaatttt atgtatttgg ataaaataat acaaatgaat atgttacata aggagtgatg
    42121 taaaatgagt agtgtaaatt ttgaggattt aaaaaacaaa tttattaata gtgacttaga
    42181 tgaaaaaata agaatatata caacaacaga aggtttatca gtagaacaat ttaaagagct
    42241 actaaaatat tatccaatac aacatttatc taaactagaa aaagcacttg gatagcagta
    42301 tttagtccaa aattaaaaag gcaatttatt taatattaaa aatatttttt attaaataaa
    42361 ttgccttttt ataataggtt taaattatta tgcttttgat atataattat agattctctc
    42421 atttccaagt tcaatagacc cagtattaat aagagtaacg attccttcta tatctctagc
    42481 agttaagtat ttatatggaa ttaataaatt ataaccagta ttacttagta agttttttaa
    42541 ttgggttatt tgagagatat tgaaacattt tgcagtttta gtagattttt ttcttaagtt
    42601 agataaattt tcattttgaa tatttattaa agtttctata tagtttctat aagatatctt
    42661 aaaatttgtc tcattgacta ttttttcatt aagtatacta ctgataaaat gttgagacaa
    42721 tatattatct gcaaatttat ctgctgttaa ttttccagat gttatgcctt tttgatattt
    42781 tctaagaatt tttttagtat agcagtatat tttatttctc gtttctaaat cgagttgttc
    42841 tatttgcaca aaaccacttc cttctaatta taaaaatatt tattaaaaat ctatacttaa
    42901 ctaataaagc ttcaataatt tatttacagt aaatactaaa tcataaaata tttgttattt
    42961 tttattatta ccaagtataa aaaatattat aattgtaaaa cttacaaata tatgtaaaat
    43021 atttaggagt gatgaaaatg ttaaatggga taataaagct tttttattct gaaattgtaa
    43081 aaacaaagat taataaccta gactcaagtg gatattcttt aagagatgaa tgtaaagaaa
    43141 tcagaaatat aactctaaag atagatagcg tattttttaa gcttttaaag ttggcttttg
    43201 gttcaatact gcttatgata gcttttaatt taaacatgat tctaggtata ggagtgtttt
    43261 taattggtgt agtttatata ctatataaaa gaaatatgga agaacaagta aaagagcata
    43321 taaataatgt gaaaaataat atcgacatct ctaaaataac ttttatggat gaaaaaggga
    43381 gaacaggtct aaatgcattg attacattac ttcttatagg attattgaca ggatttaact
    43441 ggatattagt tgctagtttt gtcgttgttt ttgtttttac tataaagaat gtatgttaaa
    43501 atactaataa atagtttaat atataggggg agaaattgag tatgactaat ggcagttata
    43561 aaatgaagaa tttagtggag atatccgcta tggtgacgga atctacagat ttttttaata
    43621 taaaagatga cataatagaa aaaatgttag aagttgttca ccctacaaag gcatgtgtaa
    43681 atctatttta taaaaatgat tctaaatatg cctacttagt atgttcgcaa accttagaat
    43741 atgtacctca aatttttcca ataaatagtc ttaaaggtgc aaaaattgat tttgacacat
    43801 atccagagta tatacatgag gctgtaaatg aaaaaaagat aatatatgtg gaaaatgtat
    43861 ttgaagattc tagagcagaa ggtgaacgag atttagcaaa agctgaaggt tatattggga
    43921 gaatagtttt tccttttata ataaataatg tagttgttgg atttatgact tgttttttaa
    43981 aagaggatga ttatataaca gaacaagata tagattttat atcatctgtt gcatcactct
    44041 taagtttatc aatagaaata actaataaaa ataataatgt acaaatttta attgataaac
    44101 taagaggctc aataagtgcc ataaatgaag ctactaaaaa actatacctt aataagagta
    44161 ttgatggctt tttggaaaat ttaagtaaac aagcgtgtaa tattactaat agtaaagaag
    44221 ctttaataat tataaatagt catcataata aggatactat agttagttgt tataatgttg
    44281 agcaaaaaaa gaaaacaaat ttatatccta tgattgatat gtttttaaag aaagagtcat
    44341 tagggggata cctaaataat ttaaaaccat gctcaaaaaa gggcataaat ttggaaagtt
    44401 atatatatta caaacttcag gataaaaatg agactatagg atgtatagtt tgtgcaaata
    44461 gtaaaaacta tactagtgat gatttaaata ttcttagtat attgtccaaa caagtttctg
    44521 ttgggatgca attatatgaa cataatgaaa aggaagtaaa acacaaagtt cttgaaaatg
    44581 agttgaacat attaaataaa caacaacagc ttattatgga taaaggtaaa atggattgta
    44641 atgatgagaa agaactttat ttttaccata aaccagcaag ggtagtaggt ggagattttt
    44701 attatgctac aaaagtggat gacgaacata tagtttatat tgttgctgat gtaatgggac
    44761 atggaatggt atcaaattat gttgttgcaa tgataaaagg tgcttttaaa gtattatgca
    44821 atcaatacaa tacaccaaga gaaataatga caaatttaaa taggatgtta tatgatgaat
    44881 ttgacaagat gggagtattt acaacttgtt tgataggtat tatagatact aaagaaaata
    44941 tgattactgt gtctaatgca ggtcactata gtcctgtagt tgtaaagaaa gatggcacaa
    45001 tagaaaataa tttaaattgt aaaaagggca tacctattgg aataatgaaa gatgctactt
    45061 atgaaaataa tactctttca atggaagatt atgcaatggt atgtatgtac actgatggaa
    45121 ttttagagat aaaaaattca tcaaaagaag agtatggtat aaacagatta gaaaactttc
    45181 taaaagaaaa ttttagatta aatcaacagt taatagtaga aaatctaaaa ttggatttaa
    45241 agaatttctc atcaaaagat aattatgatg atgatatttt aatagttatg ttaaaggata
    45301 ggtagattga ttatggattc aaacgataaa aaaggactta aaagtacaga gattataata
    45361 ataaatggaa gtccaagaat taataaaaat tgcagtttta tagcaaataa agtaaaagct
    45421 atgctagaag agtgtgggtt aagttgtaaa atatttaata tctacaatat ggatattgaa
    45481 tattgcacag catgtggatt ttgtgaaaaa acagggtatt gcaagtttaa tgatgatatg
    45541 aagccattat ataaaatgtt tgatgattca aaaggaacta tagtaataag tcctgtacat
    45601 tttgattcaa tatcagctaa attaaagact gttgttgata gaactcaagc tatatatgca
    45661 agtaaatatg tattaaataa accttcaatt gatagaaata agaaacggat aggaatgtat
    45721 attgcagtgg gggggagtaa atcatatgat tcacagttta aaggtggaca gattgttatg
    45781 gattttttct ttaagagtat aaatactaaa cttttacaca atatatattt aaacaataca
    45841 gatgatgtat cttatataga taatcaagaa tttaaagata taatatataa aaatttaaag
    45901 atatatattg attcaatata atttgatttt gtaggtaata ttatgtataa ttgtattgca
    45961 ataatttaaa taattaaaag gagggaattt caatgaaatt accaatagca ttatctaata
    46021 aacatgttca tttgagtcaa gctgatatag acgtattatt tggggcgggt catgagttaa
    46081 ctaaatttaa accattatct caaccagggc aatatgcctg tgaagaaaaa gtggatttag
    46141 taggaccaaa aggaacttta aagggagtta gagtactagg accagctaga gctcatactc
    46201 aagttgaaat atctttagca gatggtttcc cattaggagt aaaagcacca ataaaagaat
    46261 ctggtgatat agctggaact ccaggtgtta aattagtagg acctgctgga gaagttgaaa
    46321 tgaaagaagg agttatagtt gcttctagac atatacatat gagcaatgaa gatgctgcta
    46381 aatttggtgt aactgataag caaatagtaa aagttaaaac atctggacca agagcattag
    46441 tatttgaaaa tgtattagta agagctagtg atagctttaa tctagaaatg catgttgata
    46501 tggaagaggg aaatgcagca ggcgttaaaa atggagattt agtagaatta atagttgatt
    46561 aatattataa atagacttta tgctagtaga tattttattt gctagcatat ttttaaatgt
    46621 aataaatttc tataggataa atttaatcga ttagattaat ttataaaata aaggggtatt
    46681 ataaaaaatg taata
//
LOCUS       DAGQWR010000009        45221 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.009, whole genome shotgun sequence.
ACCESSION   DAGQWR010000009 DAGQWR010000000
VERSION     DAGQWR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.009"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            68..1255
                     /locus_tag="MIW95_000396"
     CDS             68..1255
                     /locus_tag="MIW95_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBY3149753.1"
                     /translation="MEKKEILMQPNNLIKSKYDFTNVENKLFYKILFNAQKQQNSSYV
                     TTISKEELKVFMKNNNDYEHKNIKEILNMFQQSVLEFDYIEETTGKLKTFGSGLINTY
                     ELDHTDQIYTIMMHEVLYNHITDFVKMQKKKNGYTAINLSVLFNFRGAYTQRLYTLFR
                     LWSRENKEVEIKYKLDELRFYLKLKDNVYPEYKYFKQNVLKRAMNEINKKGNMVVSIK
                     EEKRKNRKIDEIIFSVIDYEPRKYFDKDILVEDYIPKKKQDNEVSFSFYIPNENIFTT
                     GTIKLFKEDFKEFDFKKDVYLNAFNKSIAIAFERDNTDIINTKNYDFFKSTLSNKISE
                     VIIHQKDDLKFKEELDKYWDDEDVKKEEREVDYSKDPVRLETVRRNLFDQGLVNEEGY
                     YYK"
     gene            1275..2075
                     /locus_tag="MIW95_000397"
     CDS             1275..2075
                     /locus_tag="MIW95_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBY3149754.1"
                     /translation="MYYVYKYIDPATEECLYVGKTEDIYARHSSHLSNKEEDWCTKEL
                     ILKYIELPRRYNMDFFEVYLINELNPIFNKLSKGDMDTEHIFFDYKEEDWSTYSEEDF
                     KEKTKGKRRFRERKIDMKYEVSKKSINFLKRIEKLDAKVEMLYENNVLSVLYNFENMD
                     IEFCENELDINLISYDFETLERFGSCSLISVYPRWKEYTATNKKILINITLEIRIGSL
                     ANLMELMPTFEYKITEIFQQIEDIFHILEVGYTWQELFEIYKGCKYDN"
     gene            2095..2829
                     /locus_tag="MIW95_000398"
     CDS             2095..2829
                     /locus_tag="MIW95_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBY3149755.1"
                     /translation="MYYVYKYINQDTEECLYVGKTENICDRHASHLSNKKENWCNKNL
                     RLEYMELDNKYTMDFYEIYLINKLEPKFNITGKGEMDIAKTSFSYNGQWVIYLERDLM
                     SSLASKRYGINYEVNAKMLGIMRKLKKISSNEEIFIGNENIRIKYYFETVLEGINLEP
                     CILSVAYKTLKESTVGTVISVKREYLSENVCLIIDSIFLNEIMKDPLMSKSDIKDLNS
                     QVNDILKIIGVDCEWNKLSDYCSANS"
     gene            2949..3101
                     /locus_tag="MIW95_000399"
     CDS             2949..3101
                     /locus_tag="MIW95_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149756.1"
                     /translation="MDNFLFNVLASLTASVVVYLISELFKKAKSHSRAKSDLQVEFKF
                     IFKFKK"
     gene            3489..3791
                     /locus_tag="MIW95_000400"
     CDS             3489..3791
                     /locus_tag="MIW95_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149757.1"
                     /translation="MEVNLHTGKDEFFKNHEVAKNYIGGLYKLIDVYKFSMESVSVLT
                     RIDLDKLNNFYAGEASLNYDELCLIESVIITPLVGAFETAEHNYNMIIKARKERKL"
     gene            3826..4284
                     /locus_tag="MIW95_000401"
     CDS             3826..4284
                     /locus_tag="MIW95_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149758.1"
                     /translation="MKKTIALLLTLCAVMIPFTAFADGNDYEGTGNSQTVELIGQPNS
                     DSPSTKSIVVPGGTANLTAMSGGQLSWRVTSTLGNILGFEGEINVYKAGKIVSSQLVG
                     FRLAGTTKTGVVSFKGLKKGSYIAKFKGKGVCTGGIILFSSPAEASFTIR"
     gene            4514..4717
                     /locus_tag="MIW95_000402"
     CDS             4514..4717
                     /locus_tag="MIW95_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149759.1"
                     /translation="MSRIVLTFKNNDKEKAIEKFLDEKLSATAYLKELVWEKMNEKKD
                     NAVVEQKKEIEDPANNFDFGSLE"
     gene            4736..5641
                     /locus_tag="MIW95_000403"
     CDS             4736..5641
                     /locus_tag="MIW95_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBY3149760.1"
                     /translation="MSKLGIDIGNYAVKTSTDDIFESKVTEVKNFGSDSDSIKIGNKT
                     YYLGEGDEEINIVKYEKENFLPLLLGAICRNTDDEVIDLALGLPVKQFGGLRKDLIEK
                     LQGKEYHVEFEKGNETTKRDITIRSVQVFPEGVTGYLYYAKDIVDQIAGRDVVLVDIG
                     GKTTDIALVQGNKATDPYSVNVGTINIYDAIKKSLEMDERFLGKVEIKREKIQDYIDK
                     GFYLNGEKQDIKKNIDASVGLFKQIYNELKLNYPISTSAVVVMGGGAKLLGEAFKKNI
                     PGIIVMSDVDKHVFANAKGYKKMMK"
     gene            5723..6238
                     /locus_tag="MIW95_000404"
     CDS             5723..6238
                     /locus_tag="MIW95_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149761.1"
                     /translation="MTWSDLKEKNINEVVDCINNKLSEFESLKKVGDELGANESTIRK
                     WLNKKGYKRVGNRFLEDNKSYDDKYQIDSINSKIDDDIKYLSNEIDNIKSVIEWFKTK
                     DDKCHIDSIKENDISIDLPDQSIKRTTIRVNNTVWEMFNKFADENKHYDKHDLLSQLL
                     LESLLKYVKSR"
     gene            complement(6249..6584)
                     /locus_tag="MIW95_000405"
     CDS             complement(6249..6584)
                     /locus_tag="MIW95_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY3149762.1"
                     /translation="MLIQSLKRGELIVMKIFWKRNSRISRKEIIRICNEKHKWQKSTT
                     KILLKRLVKKRVLLKRIKIFHIYYIPIVTEKEYFKYKINDLEPDYLDGYFIRLMSTVH
                     KFKNLDEED"
     gene            complement(6918..7687)
                     /locus_tag="MIW95_000406"
                     /pseudo
     CDS             complement(6918..7687)
                     /locus_tag="MIW95_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894430.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(7746..8582)
                     /locus_tag="MIW95_000407"
     CDS             complement(7746..8582)
                     /locus_tag="MIW95_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY3149763.1"
                     /translation="MKICITVGHSILKSGACTSADGVINEYQYNKSLAPVLADIFRKE
                     GHKVDVIICPEKQFKTKSEEKSYKIPKINSGNYNLLIELHLNSSGVGAFGTEVFYYSE
                     KGKEYAQRVVDKLSKPFIRKKGDKEVGNRGAKLDKSLYILNSSKPTAVLIESFFCDNK
                     EDYEKAKKLGHEGIAKLIVEGILNKNINNEGVKQMYKHTIVYDGEVDKIPATVVGWGY
                     NDGKILICDIKDYVPGQTQNLYVVGGGACEKIGSITKEHYTMIKGNDRFDTLYKALDF
                     IK"
     gene            complement(8582..9073)
                     /locus_tag="MIW95_000408"
     CDS             complement(8582..9073)
                     /locus_tag="MIW95_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBY3149764.1"
                     /translation="MHLKDLDNSRSFLIQKLGGNMNLTIVFLATNIFIKLVILAIAFD
                     TLLGCLRAIKTHQFNSSFGINGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWIDF
                     LRLNHLGISEFFSILFILYESVSILKNMYLCGLPVPKKLKEKIGNLLDTMTDELNIKG
                     GNK"
     gene            complement(9081..9299)
                     /locus_tag="MIW95_000409"
     CDS             complement(9081..9299)
                     /locus_tag="MIW95_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149765.1"
                     /translation="MKKIIVICILVLMLIIPTMGEIFIMVENIKLEGLEQNPTSCYIS
                     NPGIMGEAKCDAAYFIIAADFVNNLKVK"
     gene            complement(9397..9579)
                     /locus_tag="MIW95_000410"
     CDS             complement(9397..9579)
                     /locus_tag="MIW95_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149766.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFELDDVTNTEYRKAVED
                     YIIRESGVVEGAEPTK"
     gene            complement(9579..9872)
                     /locus_tag="MIW95_000411"
     CDS             complement(9579..9872)
                     /locus_tag="MIW95_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149767.1"
                     /translation="MNVPNRVIYDQTGKIIFETGESCGDVLPHNKITELDYIDIPYGS
                     IDYIKNRIIGINIETKQPILEEIPVYVSEEKKRIQELENQVLLNENEKVGGLL"
     gene            complement(9888..11375)
                     /locus_tag="MIW95_000412"
     CDS             complement(9888..11375)
                     /locus_tag="MIW95_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149768.1"
                     /translation="MSTTVLERTVKRRRGYYRMTDIHASRLTYNDGSPYYTDFVAYYT
                     LDQYERVSISATKKFVAYSTRACQIINGREVDISRNFTQETTVQFVPDPTIFISNDLG
                     VIGNACSINYRISDSDSSVRFKIIEKINGVKIAEKNNVVDGNYELIITDELLSELAFN
                     SVNNITIELDNGYGGIFLDKTVTFTKGNTKPKLNITSYNSTSATFTAIDTDNNLSKIE
                     WFIDDVLKETITTDLTTEKTINYELADNAIHTLKIVATDSENATAEKVLSISKEIMPL
                     QSDASLSDISTKLIEIGEGFRNGKTSIINTLALKNIEASLNNTLVELSEKIKQSFDSG
                     DASLQDLMNQLTQANNTISQLNSKYKVASGTVTSFADSAKIAYPYLTDRTFKPGTWVK
                     ISNLDFKPNIFFADFDYYDTEYKNNYKLFLFACRGVATQRGVDFSSVTAFIRKNSDEN
                     FHANGWLYNNSEGDVYFNNTGVQIPAYNFDSTQRHIYKWYAIKFI"
     gene            complement(11395..12177)
                     /locus_tag="MIW95_000413"
     CDS             complement(11395..12177)
                     /locus_tag="MIW95_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBY3149769.1"
                     /translation="MAQAQYYTLLTEIGKAAIANATALGTRVDFAKIKVGDGGGSAYI
                     PTETQTELKNVVWESTLEHVQADEKNPNWVVIQKTITGDTGSFTIREIGVFDSKDQLL
                     AVSSYPETYKPAPDSGTVKEILIKIILAVSNTASINLKIDPTVVLATLRDIQDLDAKI
                     DTTKTELTSNIETTKTELNTKIGDTTQLTTTDKTNIVSALNEVKSSVDSIETTAEKTS
                     IKDTDNLFSSDNVEGALKEVMQEVKGNRSSIISTVNNNLIPM"
     gene            complement(12178..12792)
                     /locus_tag="MIW95_000414"
     CDS             complement(12178..12792)
                     /locus_tag="MIW95_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBY3149770.1"
                     /translation="MKLIDYLPSFANNEIDIQIQEALENELLTLIDEKDDLLEQFFID
                     TATWGLDDWENLLSIKVNYKLDFDTRRSNIKAKMRGKGTTTIEVIKAISEAYTKTNVD
                     VEVFSNLFSFTLSFITNDCSYNTILELDKKIEEIKPAHLEHKFEMILFNENGLYAGAM
                     SSAGETVTIYPYTPKNIECFGEIILASGNNKSAERVTLYPQEVI"
     gene            complement(12794..13846)
                     /locus_tag="MIW95_000415"
     CDS             complement(12794..13846)
                     /locus_tag="MIW95_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBY3149771.1"
                     /translation="MYSGQSFSTLNNRTLNNINLPLYKGQGSSLYNIVSPINSELAQL
                     YIELSYIHKRVFIQDNFDDFLDRRVNEFGVYRKLGTEAIGEVIFEGKIGTKIPNGTIV
                     SHSDLLFVVIKDVTIDEDSKLNVSLIQALEVGIKYNLSANTEFKLIEEINGVTKIYNE
                     LDLKGGTEIETDEELKERFYKIQKNQATSGNKAHYQSWALEVEGVYNAKVIPRWDGPG
                     TIKILIYGQNNQSVDNEVLQRCIEHIEEEKPIGPTVTIVTPSTFDINISATLTLENGY
                     DIESIKVVFLDIINSYLIENSREIIYIKIMSLLASIEGVHDIKNLLVNDDVKNIIVDE
                     EKVPAVSSAIFDIEVS"
     gene            complement(13839..14273)
                     /locus_tag="MIW95_000416"
     CDS             complement(13839..14273)
                     /locus_tag="MIW95_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBY3149772.1"
                     /translation="MNNTIFPFMGVPEDYISPDNEELPIFKEFAWDFNKDEKIIENGD
                     FKIVERNEAIKVWIYKTIKTDKYVHLIYDWSYGTDIKNLIGQKYTKGLTESEAKRYIQ
                     EALLTNPYILEVNITNAEFEDDNLSISLNVKTIYGEEEITFV"
     gene            complement(14266..14598)
                     /locus_tag="MIW95_000417"
     CDS             complement(14266..14598)
                     /locus_tag="MIW95_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBY3149773.1"
                     /translation="MADPINEFIGIIRQEGKFHNEPSFFIGKIKSKLPDLKIEINNII
                     LEKEDILIDSWMLDRQIELFDTETSQEHKHEIKNPFIDTFESGNTVIMFKIGEKFAVV
                     SKLVSLDE"
     gene            complement(14612..16129)
                     /locus_tag="MIW95_000418"
     CDS             complement(14612..16129)
                     /locus_tag="MIW95_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBY3149774.1"
                     /translation="MIIDRSKDSSNNNIDFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFLENKLPLGNLPKTGVKYTKMFIGVTGYDTIMSVYTEASKTTKKKYMIESNLDKFN
                     VIEKGVVTLNITFEEGFNLINTNFSESMENVKNKVLVVDQYGNKISEKINDSIFKDVG
                     VIMQKVIQQQENSTIDIDGEFKGIEKTCSLKGYGDITCVTGRGVKVKDSYTKLIGLFY
                     IDTDKHTWQNGDYQIELELNFENIMDEKSAGQDEQKEDGSEYTGGTEYSAEFTAYCPR
                     KSEGGDKDCRGKKLDPSKKTCAAPMVGSYEKSYYTKDFLNKHPLMRYGDEIQLVTGVS
                     SRDGTYKVNDNGPAIIIEKDGTYHIDILFGNVEEANNFGRRKGKIIIGGYSGNMTEKA
                     KTVISEARKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVNLPRTSSQQSKVGKKVEK
                     NNLQAGDLVFFHNPVSHVGLYVGNGEFLHAPQTGDVVKISKLSNRKDFNTARRVL"
     gene            complement(16370..17005)
                     /locus_tag="MIW95_000419"
     CDS             complement(16370..17005)
                     /locus_tag="MIW95_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBY3149775.1"
                     /translation="MEIWLRQANNTFRFPVIPPSFEINGSATINTSNILSVGDIAVFG
                     GLGLKTIELSSFFPNQEYSFCNYNGFPKPYDCVNLIESWMKEGYILRFIITETNINFE
                     CIIADFNYREQDYSRDIYFTLSLKEYRRIQISKVNINNDEKLSSEKNVPLTKGFDTKQ
                     KTHKVVEGDTLFKIAKKYYGNGDLWEKIYKANEDEIKDPSVIKNGWVLIIP"
     gene            complement(17087..20476)
                     /locus_tag="MIW95_000420"
     CDS             complement(17087..20476)
                     /locus_tag="MIW95_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBY3149776.1"
                     /translation="MSSNAKVLQAIIKMRDEASSTLKRVAANTRALHDSSSQASRALN
                     NVQSNLKSVGTSALKAGGLITAGVGGAFAAATKVGTEFESAMSQVKATFGNSLKPGDF
                     EALSKAARDAGKATTKTAADSANALQYMGLAGWDVQKSMHALMPVLNLSEAASMDLGL
                     ASDLVTDSMSSMCLEVDKVSKSGKVLAGAGLSEYLDKVANASTKSNTSIQQLMEAFNV
                     AGGTFSNLKVPLSEATAIMGILANRGVKGTDAGHALNSIMVNLTTGAGQAGKAMKKLG
                     VSAFDNQGKFKGMSNVLKEVANKTKGMTDEQKNYYLSAIGGKTQLKTLQKLLDGVGNE
                     YDSLKKKIDNSKGALKTMSDVMKDNTKGGLATLKSALEETGITIYYQLKPAIASVVDN
                     LTKMTVWFNNLSEPIKRVITNVMLSVGAFGVLLLTIGAVSLIVSKAIGVFGFFVPIIL
                     RAKVAFRAFVIGMKPLSAVFLAVMGPVGWTITAVIALAVAFTIAYAKSEVFRNKVNSL
                     VKSFTEFAKPIVNFCMPALKQLGEKFKELLSAFGPLFASILNLGSVIMKVLGPVLIGV
                     IGFHIAKLVLILSTLATAFKAVVDTITNVLNGLASILNGVFNLIIGIVTGDSKKMMNG
                     VKQIFDGGVKIIKSIWKGFIDFVTAPIQAIADIADSLFKDKAKWLTDKWKDLRTFLEN
                     PIEATAKISTKTNVNIPGSKAADAAGKSTPNTGGLKQTIANLKGSFSDFKTFIKPMTD
                     SISQGVESGKTKFTELQNKILNDVKPALKVLEDSGVNFKDKFKESITSIKDKFNELKN
                     ILAGNIKTNIDNLVKAFEPLKPHLDNLKECFDKVKKAITDFFTPAKQVGDTVNKVSQP
                     MDSVKKSTDGIKLSFQSLTQALAPVKAWFQGFMPFLSQIGQQLLPSIGIAIAGVVGGI
                     ILAFTWVLNTLTAIITTVSGIINGIMSFISGIINVIMGLITGNADQTMDGLKQIFDSG
                     VGVIKSIWNGLVDLVSGVLEPVIDLADSLFKDKVKGVKEAWNKLKEFFHNPIKGMIEI
                     VKSGPSLQIGKGADAAGKSPRKAFGINRVPRNDYLIRAHEGEKLLTKQEANQYKRNSS
                     NGSLFLDKLADTIIVREEADIDKIVKKINKKISIANAGGVL"
     gene            complement(20480..20629)
                     /locus_tag="MIW95_000421"
     CDS             complement(20480..20629)
                     /locus_tag="MIW95_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149777.1"
                     /translation="MYLLFKYKGIMPSVSYRILRGEKEIVSVFMDQELDERKEEIKSG
                     AGRLF"
     gene            complement(20656..21072)
                     /locus_tag="MIW95_000422"
     CDS             complement(20656..21072)
                     /locus_tag="MIW95_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBY3149778.1"
                     /translation="MNTVEKLLNMDAGKLKMPSSIFELYCKKLDDILEIECNAIDPER
                     FDEIRMNSMDVDSGNLENINVYELKVNTILESCPMFRNMEVVENLGLATPKDLLKKLL
                     LAGEIDNLYEEVNKVNGIDAEGDKKRQKERAKDIKN"
     gene            complement(21088..21528)
                     /locus_tag="MIW95_000423"
     CDS             complement(21088..21528)
                     /locus_tag="MIW95_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBY3149779.1"
                     /translation="MKAKIDPSRIISGTFGECWIDGRQMMAVKGLEAKVELEKEEVKV
                     CGQLMVGQKLIGASGKGSVNLFKVSSLFGEYVESMLNEGKAFKFTIISKLQDPDSYGA
                     ERVALYGCMLDDLTLADWEAGKMGEIEVPFTFESFKYLDKITEK"
     gene            complement(21544..22599)
                     /locus_tag="MIW95_000424"
     CDS             complement(21544..22599)
                     /locus_tag="MIW95_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBY3149780.1"
                     /translation="MGLPEINISFIQAGKTFVKRSGGIVALILKDTKNIGLVEIKEME
                     DIPENYSATNLDYIKMAMKGNVYPPNKVLVYTLDTDESIDDALDFLETCEFNYLCMPD
                     EEEGDLPKIKTWIKKMREDNKIKVKAITASNSADYEGIINFTATDIEVEGKKYTSNEF
                     LPRIAGFIAGTPSTQSVTYAEIPEVTNIPKLTRAEANTRINNGELILIKESGAIVIAR
                     GVTSFTTITDSKGDIFKKIKLVDTLDQIHNDIKKIIVKNYIGKTQNTYDNKCLLIVAI
                     QLYLQELEKDGLIDPGSTIEINLDAQRAWLKENHIDVSNMEEQQIKEYNTDTIVFLKA
                     KIKLIDAMEDVYLDISM"
     gene            complement(22600..23049)
                     /locus_tag="MIW95_000425"
     CDS             complement(22600..23049)
                     /locus_tag="MIW95_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149781.1"
                     /translation="MLTNRDILIAVNEILKNEFIDIDIFINEDNIQGFEEACFFVQLL
                     PVSNNIANKILNTKKIFIDIEYYQKARKNKLNLYDIQSRLEKIFNRNIRVKDRSLTIE
                     NISPSIVKDSVGHKLTFLMTISYFEEVYFSKETYVTMENVDLKIGSD"
     gene            complement(23058..23414)
                     /locus_tag="MIW95_000426"
     CDS             complement(23058..23414)
                     /locus_tag="MIW95_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBY3149782.1"
                     /translation="MGIDNLGVFEALALDLGLNASNFNRKIKKFTKETGQELAENIRD
                     ETPVRTEKLKNGWEESQPEYDTYIVEDKVEYATLVNDGHATKNGGIIPGSFMVEKGIE
                     KTKENMTSAKANLFDW"
     gene            complement(23414..23761)
                     /locus_tag="MIW95_000427"
     CDS             complement(23414..23761)
                     /locus_tag="MIW95_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149783.1"
                     /translation="MTEADILEMTYLDKMTIIRKLKGWNEETCSNDFSNVVIAENIPC
                     AISRKEEAIVNGDIGSIVVNRKLFCRPEVDIKIGDTINTTYNHGEEAVFKVSKANIYP
                     SHMEIPITELERI"
     gene            complement(23773..24153)
                     /locus_tag="MIW95_000428"
     CDS             complement(23773..24153)
                     /locus_tag="MIW95_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBY3149784.1"
                     /translation="MSTMLDNVKLVLGIENDRYDKLIILYINKISEMVLEYCSRQELS
                     IALEGFVEEKVISILSSRFEELKKNSGLDNLEKSLKAITRGDTKIEYNALTLQTAENS
                     ISSSSFLTDTDKKFLSPFCIVSMY"
     gene            complement(24137..24409)
                     /locus_tag="MIW95_000429"
     CDS             complement(24137..24409)
                     /locus_tag="MIW95_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149785.1"
                     /translation="MFALIKNNIIKVVEDENSRDKLISKGFKIYSEEVTKENIEKKEE
                     KVKSTSNDLFNLSKPELMEIAKGKNIKGYSTKTKEELIQLLDEYNA"
     gene            complement(24423..25346)
                     /locus_tag="MIW95_000430"
     CDS             complement(24423..25346)
                     /locus_tag="MIW95_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149786.1"
                     /translation="MANVLQYVTLFQQELDMQVTVGSTTGWMEKNAGQVIYNGGREVK
                     VPKVTMDGLGDYDRSKGYTQGAVTLEYETMKMEMDRGRTFMLDSMDVNESNFVINASS
                     VMGLFQKQHVIPEIDAYRYSKIFSETAKVDERVKYSYEATVTTILSELKNQLASIQDL
                     SGDIPLIISMSSIIASILEQSSQLDDIEFGDGDIKRIVKSIDNVPIIKVPSKRMKTEY
                     VFRDGKTSGQTEGGFTEGAKALDINWLITPASAPIAVSKTDKPRIFTPDQNQTADAYQ
                     IDYRKYHDLWILDEYLKLCRASVRQAKAAQE"
     gene            complement(25375..25977)
                     /locus_tag="MIW95_000431"
     CDS             complement(25375..25977)
                     /locus_tag="MIW95_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBY3149787.1"
                     /translation="MLKKEVLELLKEIEDDAEIDSLLQSTDLFKSASDKKLTIEEFRE
                     LINNDANFKAIIDNEKNKYHSEALENFKKKDMQTLISAEVLKRTGANETEEQKAIREL
                     RESLDKLKKEKQYAEKVSKYKDILVEKKIPTNLIEYLLSDDDDKTNANIEIFENSMKQ
                     YVQSKVDERIKDGSYVPPGKDSAGSLSEIRKQIKQGLNSL"
     gene            complement(26003..26329)
                     /locus_tag="MIW95_000432"
     CDS             complement(26003..26329)
                     /locus_tag="MIW95_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149788.1"
                     /translation="MKVEILGTEYLVIEKLESEDVLLKERAGYCDHSVKEIVIEKINS
                     EEGSLKDLSVYKNEVARHEIIHAFLSESGLKSCSSWATNEEMIDFFAIQFPKIVKVMD
                     KIECLN"
     gene            complement(26388..27149)
                     /locus_tag="MIW95_000433"
     CDS             complement(26388..27149)
                     /locus_tag="MIW95_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBY3149789.1"
                     /translation="MNQKIITKLTEDIYKSAENRTKPFYKYQRENRDKLLLEIAKILL
                     THDIINEYLNINNKDKKNLRSKLNKIINEYSNSYDEEVKDIETILSDSSKNKYSNLII
                     LLGVILKIKDVSNINNKVINKIVNNKVDGKHWSDRLWKNKKGIEESLKKEIKSFLNGK
                     TSVNKIEKAIRDKYSTGAGQTSRLIENEVARCQSEVNEYFFKVQGIIKVMYCADLDSR
                     TCSDCSMHNGVIFYVNEDRPSLPRHCHCRCQYIPV"
     gene            complement(27127..28404)
                     /locus_tag="MIW95_000434"
     CDS             complement(27127..28404)
                     /locus_tag="MIW95_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBY3149790.1"
                     /translation="MELDLDLIKDIYDDWNSNKSEYDTMYKYYKGETDAISNYKMVTK
                     RSNNKINTNFLKKFINEEVAYSLANKITYTSRSGDEKIINDLEYYTCHWSKKHDSDLL
                     RYALLFGLCYELYYVKDDEIQVKIIKPTDGCHYENENGEIVCFIREFTKGFEDDTYVD
                     VYDKEYIYHFDSNFKEVESPTVNNIFDGNVPISICKRSEELGKNTIFNDIKGLQDAYE
                     TNLSDISNEISDFRNAYLTFSGCNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTF
                     VQNTLSSIKENMYEITSHINHNEKMQSNTSSLAVIARLISTEWICSQNNDSIADTLFN
                     RYKLLCIWLNKKYGFDYDYKDIKAKFTPKIPRDDLVVANILSQLGDKLSTETGLSQLS
                     FIDNPHAEIEKAKKEQEIVSEGEILLDESKDYN"
     gene            complement(28417..28620)
                     /locus_tag="MIW95_000435"
     CDS             complement(28417..28620)
                     /locus_tag="MIW95_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149791.1"
                     /translation="MGGNLLANRSKFEEVQKTLTKKGKKFNVGIEKDEKGYFAYTHRA
                     RSKSYISKQDIPIKVLKFIESTG"
     gene            complement(28621..30360)
                     /locus_tag="MIW95_000436"
     CDS             complement(28621..30360)
                     /locus_tag="MIW95_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149792.1"
                     /translation="MDNLERNIQLIFSYLAKTFIRNGASIKDAEKEANEIIKANSNNL
                     WGVNGLAYQLGKINLEFFCMYFMQDTYLPKEDNAAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGRKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKRFSDDVKYAAQKPVIRNGKTIKRGTTFIALGTLQHKECF
                     YSRLKKLPTWKFKCEKGVLVDNVDELFNSGLWLKFKEILFDFKNTNHLEDAKEFYWEN
                     EKEMKFPILWPSFWDCLDMALSYYENPTSFKQEVQNDVNSIGEKWFKTVRTETREEIE
                     SHTFKKTMLLCDPASAGGSKHDYSAFLVGSESENGLLYGRLAELAKINARTDFDKYID
                     HMIYLLKVYSDITHIYIEKNTFNGADANQLEFKIKNDNVLSYRDIEIINEQQKKNKDD
                     KISTLIPVLNKGQMIFAEEDKEFIQQILDFTGQKYSLHDDAPDISSEFINRINNIKVS
                     ETVNIFDRKLLGF"
     gene            complement(30915..31373)
                     /locus_tag="MIW95_000437"
     CDS             complement(30915..31373)
                     /locus_tag="MIW95_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3149793.1"
                     /translation="MDVNEVEIIDSSYEKGKELTEDQCNMVTLLITGATVTETARIIG
                     VNRKTIYNWMNKEHVRKEMDRRKQELTNQGNLMILKDLDSYINNIKELASDKSDKRVM
                     LAANQYLINRIYGTPTSTVIQAEDNSVGMGMDATEIEAAIAKIRIRTSKK"
     gene            complement(31587..32231)
                     /locus_tag="MIW95_000438"
     CDS             complement(31587..32231)
                     /locus_tag="MIW95_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBY3149794.1"
                     /translation="MEAHSGQKVNPIKDIEDVFRLLNFLEEWNERNYLLALFGMCTGL
                     RIGDILALKVADVTDVKLDKKGKKIRVSKDWIRVIEEKRDYDREVFLSDVIKSAIENY
                     TQDKPGEEFLFKSGKRKKFNRPIQTRQAGRIIKGAAEKVGIKENVATHSLRKTFARHI
                     YDEEENKTYALELIRKILGHKTIEITRRYIGIDKDEEVKAITKFTNKLKKRKRN"
     gene            complement(32235..32780)
                     /locus_tag="MIW95_000439"
     CDS             complement(32235..32780)
                     /locus_tag="MIW95_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149795.1"
                     /translation="MAWNLRSKSFLLSREVLKIKRDITEEISSAAYVPDNLKYYDSVM
                     RETHSMYGRECSLDFIKKKQEKLLKLKKKVKKNYDSNINKIDSYLKILDESIIDESDH
                     VELVVFKLYLQLENVVKVTETVNDLGFRIKTNTYARERKYSTNDITSIITDPFANVVE
                     DLKILVQDRQRKNYHGNKECF"
     gene            complement(32924..33367)
                     /locus_tag="MIW95_000440"
     CDS             complement(32924..33367)
                     /locus_tag="MIW95_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HBY3149796.1"
                     /translation="MQSSKKDNLFSSAEGKLYNYKKIKAEIEKIDIDMQMIKNDYRGC
                     SGVEIKEKTSKTYDIKSVIEIETEEKEKKIALKEKEKRHKELIIEKIDNAMKILSEEE
                     KKIVQYKYFSSSRTSWEYVGRMIGFSASKCKQMRFDIIDKIKGLL"
     gene            complement(33475..33732)
                     /locus_tag="MIW95_000441"
     CDS             complement(33475..33732)
                     /locus_tag="MIW95_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149797.1"
                     /translation="MLIEVTENNRELKVGDIVEINGGNKLYMILSLKETIGGYVIINM
                     QNGYGSFGAYKSLSQLELDLKVRGYKLYSSDDYKLQLVPKE"
     gene            complement(33752..34237)
                     /locus_tag="MIW95_000442"
     CDS             complement(33752..34237)
                     /locus_tag="MIW95_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149798.1"
                     /translation="MDLKLISSIPNWKVEDQLAKIDEEVTEFKEAIDTGMTKDIIAEG
                     LDVCQTILTMFQILELEDYIAEGIEIHNKKLNGRGWDLKEIGDGILNLIKNSMINELI
                     NMNNKLKCVDEKEFNSKIQFLDVNKLWDLCVPLVNLSAMKGINENIDEMKMGEFLKNI
                     N"
     gene            complement(34310..34639)
                     /locus_tag="MIW95_000443"
     CDS             complement(34310..34639)
                     /locus_tag="MIW95_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149799.1"
                     /translation="MSCVIKCDFCGETIAEILNIKVFRIDDEGNIMSVGKDVCDKCYD
                     RLFGSNLNKKMTNYEKIKNMSKIEMAEFLANGGSGCTSCAYDFQDCLGGCLEGRKKWL
                     ESEEGQI"
     gene            complement(34828..35412)
                     /locus_tag="MIW95_000444"
     CDS             complement(34828..35412)
                     /locus_tag="MIW95_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY3149800.1"
                     /translation="MMKIFHFKLDGDYMRSLEEKNKLFEDNINLVHFIINRYFKSFLR
                     RYPYLKEDLFQEGYIGLYKTTCCFDESKGKFSTIAFSYISGHLKRFTTGYVKKHYRND
                     IDSFEKCIYRDNYGEEIRIEDRLAGNENVDIENVRVIRSCIKRSEIKDIQKIVCLREK
                     GYTQQEISKVLGTSQTSIYRRLKKLKTEINILGK"
     gene            complement(35390..35761)
                     /locus_tag="MIW95_000445"
     CDS             complement(35390..35761)
                     /locus_tag="MIW95_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBY3149801.1"
                     /translation="MNNVVLVGRLARDPELRYIPEYGTPVATFALAVDRGYAKKDGTR
                     EVDFIPIEVMGGSAEFCANYLAKGRMVSIQGQIRIEKYEKDGEKKTFTKVRTKIVNAL
                     DHKPKDEEIGFQVLDDEDIPF"
     gene            complement(35758..35940)
                     /locus_tag="MIW95_000446"
     CDS             complement(35758..35940)
                     /locus_tag="MIW95_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149802.1"
                     /translation="MKSISHMNILERAEFTDKIANAIMSANKEIERGCAFNEAVEIVK
                     QMEYRENKYNEGDIAI"
     gene            complement(35998..36702)
                     /locus_tag="MIW95_000447"
     CDS             complement(35998..36702)
                     /locus_tag="MIW95_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149803.1"
                     /translation="MIRNAMEYLVKLGKRETVCVDGFKYTTDALEIIKEPKANELEIT
                     TLSGLVDYIKSGIDHEEDELLLIQVLTPRKVLLKSALRKNRDRETYIECVALLPDKRF
                     DRPLDLDTFNIMLQSAFIKNNDRDTLLKIAGNIQESTVKNVGDDGVSQSVTIKTGVAS
                     VSEAIVPNRVKLIPYRTFQEVEQPESEFIFRMSTGPAAGLYEADGGAWKNQAMLNIKA
                     YLQEELKDFKNVNIIA"
     gene            complement(36752..37138)
                     /locus_tag="MIW95_000448"
     CDS             complement(36752..37138)
                     /locus_tag="MIW95_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149804.1"
                     /translation="MIALEKFAGGVLKEKFNTELQKVLDNIADPNTDFKKTRKISLEI
                     VFKANEDRDLAEVDIKSKATIVEAKATTTKVIIGKDLETGRVEASEFKNQLAGQLSID
                     VSDSNETDEIEENSSGVIDFRNASSK"
     gene            complement(37513..38844)
                     /locus_tag="MIW95_000449"
     CDS             complement(37513..38844)
                     /locus_tag="MIW95_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY3149805.1"
                     /translation="MNYSLEAEQNILGSFLINDSISYKIRELKENDFYFESHKIILSC
                     MKKIIDSNKPLDLLLLKNELEKIDRLVDIGGVSYITSLTTIVITTSNIDHYIKIIKEK
                     LLKRQISELANELINNSNSDTSIDELIIDINDLKALVTTSSSVNDNYIDASKIKREKG
                     VHKSIDTGFNKLNNMLDGFRYGTLTILTGKPASGKSTIVNQFIAQAITNGEKAFLYSG
                     ELPSFMSMDWFRKTVANDYHIKEYKSVYGGTYTDIPDYAVELISDWIEDKFFLYDEDA
                     ISDEVNLLNTIEHLYLKKGVRFFVLDNLMTIKTGNKADKYERQEQIVSNLKNLAKKYN
                     LVIVLVAHPRKNMGDMKPTMYDVSGASEIVNYADYILSTYRVVDEEEETDDTYLLILK
                     NRITGKQNISFKMNFSERRKRLYTTSEELNRDYKYDVNKQYVQVEIPEDVF"
     gene            complement(38866..39183)
                     /locus_tag="MIW95_000450"
     CDS             complement(38866..39183)
                     /locus_tag="MIW95_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149806.1"
                     /translation="MERKKHELTDQDIQRLIEGLRTDPKIREMIFESKDCETRTSTRS
                     KCEQFVEEKLAYRSYGFETPKYYAKLIADIIDILVYKNVPRDRICGVLYDVERVIPLV
                     VTF"
     gene            complement(39501..39761)
                     /locus_tag="MIW95_000451"
     CDS             complement(39501..39761)
                     /locus_tag="MIW95_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149807.1"
                     /translation="MKEEKLELTEKELHCIARHLQNEIMEMVFRGNREAPTSCEVCDY
                     LQECEGDFTCMYHSFTKLSKITGVKVTAFKYCFPDENPKATI"
     gene            39924..40136
                     /locus_tag="MIW95_000452"
     CDS             39924..40136
                     /locus_tag="MIW95_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149808.1"
                     /translation="MNMCKIKTLREKCSLIDDKVEFSGEYKTFPGFAGSKLLECECNK
                     NYICKIQIMTANSNCPLFKKARQLQY"
     gene            complement(40155..40373)
                     /locus_tag="MIW95_000453"
     CDS             complement(40155..40373)
                     /locus_tag="MIW95_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149809.1"
                     /translation="MADKTVELTEKDLHCIARHLQNEVLEIAFRGNREAPTSCEVCDY
                     FEECKDYFTHIDTFIKLSEMTGVDIFTK"
     gene            complement(40400..40585)
                     /locus_tag="MIW95_000454"
     CDS             complement(40400..40585)
                     /locus_tag="MIW95_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149810.1"
                     /translation="MNEKEEAVYLNSSNVKLFLGVRDIKEFEELIENVNERICQLQES
                     INKLAQFNIEFEIKSDI"
     gene            complement(40614..41345)
                     /locus_tag="MIW95_000455"
     CDS             complement(40614..41345)
                     /locus_tag="MIW95_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3149811.1"
                     /translation="MDLLKDARKREWFWLENDLVDREDLGIYEKMIYIVLARYSDNES
                     CCFPSYKTIALKCGCSERQAKSVVKILENKGLIKKENRIKSNSNEKESNIYFVLTAKL
                     GGEYDAQQVVNMMHNPSAPHALPVVHEVHSKKTYNKKTYIKSNTTPQNEPKTDYLDLS
                     FLDLDIEKVKLTKDEYDKLISKFGKKYIHDKIVSLENYIVNGKGSRYKSHYRALLTWG
                     NADTSKGILQPVTKAKNPLSGFKEL"
     gene            complement(41375..41593)
                     /locus_tag="MIW95_000456"
     CDS             complement(41375..41593)
                     /locus_tag="MIW95_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149812.1"
                     /translation="MNDEMITKMFSLNKTYKMTSIRLNLEVKGVITHIDKNEIVLEYY
                     DNELECITTKILTIDDIKYNDYNLKPLH"
     gene            complement(41610..41786)
                     /locus_tag="MIW95_000457"
     CDS             complement(41610..41786)
                     /locus_tag="MIW95_000457"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149813.1"
                     /translation="MKKVDTKKISKSIKESTEFGILYSSLKDKQRDRVLGIMEGMKIA
                     NELSENNFMDIKHS"
     gene            41945..42316
                     /locus_tag="MIW95_000458"
     CDS             41945..42316
                     /locus_tag="MIW95_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3149814.1"
                     /translation="MENIKDESVLKTLREALGLNQSELANILKMTPSAISKIEKNGKP
                     LSDRISSQLSKEFGVREEWLKNKEGKMFADSSDDDLVAEIAANIINSDDKFMKNVIIA
                     FNKLTEDQRNFLINFMKELDK"
     gene            complement(42900..43415)
                     /locus_tag="MIW95_000459"
     CDS             complement(42900..43415)
                     /locus_tag="MIW95_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY3149815.1"
                     /translation="MSKMIQCKSCSKEIASNAKSCPNCGAKNKKPFYKRGWFIVVVVL
                     VLLYAIGTSGSGTDDTAESNDDGTSKTASVDKPEEPKKEKYEIVGDITSETDSMATYI
                     KGVIKNNSGHDISYAQVTFTLYDKDGNQLGTAMDNINDLKKDGTWKFKAMGMNTDGEI
                     ASYELSEITGF"
     gene            43789..44172
                     /locus_tag="MIW95_000460"
     CDS             43789..44172
                     /locus_tag="MIW95_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY3149816.1"
                     /translation="MKIKKIPESELLVMMYIWDSEDEEVASTEILKTLGEKYEWKKST
                     MLTFLRRLVGRGFLEVVKKDRFTYYKALIEKEEYLKVETKSFFSFFHRNSFESFISAL
                     HDDEEISANKLKSLEKFIEDWEEDK"
     gene            complement(44321..45010)
                     /locus_tag="MIW95_000461"
     CDS             complement(44321..45010)
                     /locus_tag="MIW95_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBY3149817.1"
                     /translation="MLENRKQIQKEIYLPIATYCLKDKQYYIKEYGALLIKSAENSDI
                     FNTHKNRILYKNKLDIQEIANELNVNKATIQRNIKKLEKLDFKILKIENTLNGIVYCL
                     PSENNIDLNKFALINYEMLKKMVNKFKSNTIKVYFLLKTITTETNFKPATNSFIAENI
                     GLSSKSKNNLDIITSSVKTLEENKYIETKKVNVYEYDKEKLREVPKIRKVYRICNFDE
                     WKKEIDKNKVY"
BASE COUNT        14544 a         7372 c         5296 g        18009 t
ORIGIN      
        1 agcgtctttc aaaaatagtt gaccttttta taaattaggt attataattt atatgaggtg
       61 atagattttg gaaaaaaaag aaattttaat gcaaccaaat aatttaataa aaagtaaata
      121 tgattttaca aatgtggaaa ataaattatt ttacaaaata ctttttaatg ctcagaaaca
      181 gcaaaattca tcttatgtta caactatatc aaaagaagaa ctaaaagtat ttatgaaaaa
      241 caataatgat tatgagcata aaaatataaa agaaattttg aacatgtttc aacaaagtgt
      301 tttggaattt gactatatag aagagacaac tggcaagtta aaaacttttg gaagtggttt
      361 aattaataca tatgaattgg atcatacaga ccaaatttat acaattatga tgcatgaagt
      421 tctatacaat catataactg attttgtaaa aatgcagaaa aagaaaaatg gatatacagc
      481 tattaattta tcagtgttat ttaattttag aggtgcatat actcagaggt tatacacttt
      541 atttagacta tggagcagag aaaataagga agtggagata aaatataagt tagatgaatt
      601 aagattttat ttgaaattaa aagataatgt atatccagag tataaatatt ttaaacaaaa
      661 tgttctaaaa agagcaatga atgaaataaa taaaaaaggc aacatggttg tttcaataaa
      721 agaagaaaaa agaaaaaata gaaaaataga tgaaataatt ttttctgtga tagattatga
      781 acctagaaaa tattttgata aagatatatt ggttgaagat tatataccta aaaagaagca
      841 ggacaatgaa gtgtctttta gtttttatat accaaatgaa aatattttta caacagggac
      901 gattaaacta tttaaagaag attttaaaga gtttgatttt aagaaagatg tttatctgaa
      961 tgcttttaat aagtctattg ctatagcatt tgaaagagat aatactgata ttataaatac
     1021 aaagaattat gattttttta aatcaacact ttcaaataaa ataagtgaag ttataattca
     1081 tcaaaaagat gatttaaagt ttaaagaaga attagataaa tattgggatg atgaagatgt
     1141 taaaaaagaa gaaagagaag ttgattatag caaagatcct gtcagacttg aaactgttag
     1201 gagaaattta tttgatcaag gtctagtaaa tgaagaagga tattactata aataaaaatt
     1261 ggagaaaata aaaaatgtat tatgtttata aatatattga tccagctaca gaagaatgtt
     1321 tatacgttgg aaaaacagaa gatatatatg caaggcattc atctcatttg agtaataaag
     1381 aagaagattg gtgtactaaa gaattaatat taaaatatat tgaacttcca cgtagatata
     1441 atatggattt ttttgaagtt tatttaataa atgaactaaa tccaatattt aataaactaa
     1501 gtaaaggtga tatggatact gaacacatct tttttgatta caaggaagaa gattggagta
     1561 catattcaga agaagatttt aaggaaaaga caaagggaaa aagaagattc agagaaagaa
     1621 agatagatat gaaatatgaa gtaagtaaaa aatctattaa ttttttaaag agaattgaaa
     1681 agttagatgc caaggttgaa atgttatatg aaaataatgt tttaagtgtg ctatataact
     1741 ttgaaaatat ggatatagaa ttttgtgaga atgaattaga tattaatttg atatcatatg
     1801 attttgaaac attagaaaga tttggaagtt gttctttgat ttcagtttat cccagatgga
     1861 aagaatatac agcaacaaat aaaaaaatac ttataaatat aacattagag attaggattg
     1921 gatctttggc taatttaatg gaattaatgc caacatttga atataaaatt actgagattt
     1981 tccaacaaat agaggatatt tttcacatac tagaagttgg atatacatgg caagaattat
     2041 ttgaaatata taagggctgt aaatatgaca attaagaatt ggagaaaata aaaaatgtat
     2101 tatgtttata aatatattaa tcaagatacg gaagaatgtt tatacgttgg gaaaacagag
     2161 aatatctgtg atagacacgc tagtcattta agtaataaaa aagaaaattg gtgtaataag
     2221 aatttaagac ttgagtatat ggaattagat aataaatata ctatggattt ttatgagatt
     2281 tacttaataa ataaactaga gcctaaattt aatattactg gaaaaggaga aatggatatc
     2341 gctaaaacat cattttccta taatggtcag tgggtaattt atttagaaag agacttaatg
     2401 tcgagtttgg catcaaagag atatggaata aattatgaag tgaatgctaa gatgttaggc
     2461 ataatgagaa aattaaagaa gataagcagc aatgaagaaa tatttattgg aaatgaaaat
     2521 ataagaataa aatattactt tgaaacagta ctagaaggaa ttaatttaga accatgcata
     2581 ttgagtgtag catataagac actaaaagaa agtacagttg gaacagtaat ctcagtaaaa
     2641 cgtgaatatt tatcagaaaa tgtatgttta atcattgata gtatttttct aaatgaaata
     2701 atgaaagatc ctttaatgtc taaatcagat attaaagatt taaatagcca agtcaatgat
     2761 attcttaaaa taataggggt tgattgtgag tggaacaagc tttctgatta ttgtagtgca
     2821 aactcttaac tgatattaca aaatatggta taattaaatt tagcaagaag agcgtaatat
     2881 acaatctata gagtggagtt catgtacaaa agattatcct cccaacgatt cgaggggagg
     2941 tgagttacat ggataacttt ttgtttaatg ttttagctag tttaacagct agtgtggtag
     3001 tttacttaat cagtgaacta ttcaaaaaag caaaaagcca ctctcgtgca aagagtgact
     3061 tacaggttga atttaaattt atatttaaat tcaaaaaata aactacgttt attatgaact
     3121 ccactctacg cctaaataga ttgtagttct tcttgctttt attatatcac aaaatcttta
     3181 aaatgataca attttatatc atttcttatt attaagaaat agaaaaacac tttaattgat
     3241 taaagaataa ttttatataa tcttgtaaaa atataaaaag aacacttaat atattagctt
     3301 gaaaaatatt aagtgttctt tttttctatt ctatttaaat aaaatagaat agaaaaaaag
     3361 attgaaaata ttattctatc gtgatagaat gaaaaaaaca gaatattcta ccgtgataga
     3421 atattctgtt ttgaagaaga agtaaatatt atttgaaggt taaaaatata agaacaatgg
     3481 agggaattat ggaagtaaac ctacatacag gaaaagatga attttttaag aaccatgaag
     3541 tagcaaaaaa ctatataggt ggattatata aacttattga tgtttacaaa ttctcaatgg
     3601 aatcagtttc cgttttaact agaatagatt tagataagtt aaataatttc tatgctggag
     3661 aagctagttt aaactatgat gaattgtgct taatagaaag tgtaattata actcctttgg
     3721 taggagcatt tgaaactgct gagcataatt acaatatgat tattaaggca cgtaaagaaa
     3781 ggaaactgta attgagaata atataaaaaa atagggggaa aacaaatgaa aaaaacaatt
     3841 gctttattac taacgctttg cgcagtaatg attccattta cagcttttgc tgatggaaat
     3901 gattatgaag gtactggaaa ctcacaaaca gttgaattaa ttggacaacc aaactcagat
     3961 tcgccttcaa ctaaatctat tgtcgttcca ggtggtactg ctaatttaac agcaatgagt
     4021 ggagggcagt tatcttggag agtaacatct acgcttggaa atattttagg atttgaagga
     4081 gaaataaatg tatataaggc tggcaaaatt gtatcatctc aacttgtagg ctttagatta
     4141 gctggaacta ctaaaactgg tgtagtaagt ttcaaagggc taaaaaaggg ttcatatatt
     4201 gctaaattta aaggaaaagg tgtgtgtaca ggaggtatta tattattctc tagtccagct
     4261 gaagcttctt ttacaattag ataattataa aaagtagaaa agagtgttta gaaatatatg
     4321 aaacactctt ttttttatgc aaaattataa aaagtgtgtt tttaattata tacaaaatga
     4381 taaattttat aaaaaattat aaaatattat aaaaaatatc ttataaatct attgaattac
     4441 tatgtgaaat ttggtaaaat atatgtaaat attaataaat tttataatat tttacaaaaa
     4501 aggaagtggt tgaatgagta ggatagttct aacatttaaa aacaatgata aagaaaaagc
     4561 aattgaaaag tttttagatg agaaattatc agctacagct tatttaaaag aactcgtttg
     4621 ggaaaaaatg aatgaaaaaa aggataatgc tgtagttgaa cagaaaaaag aaatagaaga
     4681 tccagctaac aattttgatt ttggaagttt agaataaaag ggggaaataa cataaatgag
     4741 taaattaggt atagatattg gaaattatgc agttaaaaca agtacagatg atatttttga
     4801 aagtaaagtt acagaggtaa aaaactttgg ttcagattca gatagcatta aaataggtaa
     4861 taaaacatat tatttaggcg aaggtgacga ggaaataaat atagttaaat atgagaaaga
     4921 aaacttctta ccgcttttac ttggggctat atgcagaaat acagatgatg aagttattga
     4981 tctagcatta ggtctaccag tgaagcaatt tggaggatta agaaaagatt tgattgaaaa
     5041 attacaagga aaagaatatc atgttgaatt tgaaaaagga aatgaaacta ctaaaagaga
     5101 tataacaata agatctgttc aagtgtttcc agagggagtt acaggatact tatattatgc
     5161 aaaagacata gttgaccaaa ttgcaggaag agatgttgtt ttagttgata ttggtggaaa
     5221 gacaacagat attgcgcttg tacaagggaa taaagcgact gatccatatt ctgtaaatgt
     5281 tggaacgata aatatatatg atgcaataaa aaaatctctt gaaatggatg aaagatttct
     5341 gggtaaagtt gagataaaaa gagaaaaaat acaagactac atagacaaag ggttttacct
     5401 aaatggtgaa aaacaagata taaagaaaaa tatagatgcg tctgttggat tatttaaaca
     5461 aatatataat gaattgaaat taaattaccc aatatcaact tctgctgttg tcgttatggg
     5521 tggaggtgct aaattgttag gtgaagcatt taaaaagaat atacctggca taatagtcat
     5581 gagtgatgta gataaacatg tttttgcaaa tgcaaaagga tacaaaaaaa tgatgaaata
     5641 aaaaaggatt tagtttatag tatgtataat agattacaaa tatatgatat ttgtaatcta
     5701 ttattttttg aggaggtttt atatgacttg gagtgatttg aaagaaaaaa atattaatga
     5761 agttgttgat tgtattaata ataaattaag tgaatttgaa agtcttaaaa aagttggaga
     5821 tgaattaggg gcaaatgaaa gtactataag gaagtggctt aataagaaag gatataaaag
     5881 agttggaaac aggtttttag aagataataa aagttatgat gacaaatatc agatagatag
     5941 cattaacagt aagattgatg atgatatcaa atatttatct aatgaaattg ataatataaa
     6001 aagtgtgata gaatggttta aaactaaaga tgataaatgt catatagata gcattaaaga
     6061 aaatgatata tctatagatc ttccagatca gtctataaaa agaactacaa ttagggtaaa
     6121 taatacagtg tgggaaatgt ttaataaatt tgcagatgag aataaacatt atgataaaca
     6181 tgatttgcta agtcaattat tgcttgaaag tctattaaaa tatgtgaaga gcagatgaag
     6241 tgagatattt aatcttcttc atctagattt ttaaatttat gcacagtgga cattaatcgt
     6301 ataaaatatc catctaaata atctggctct aaatcattta ttttatattt gaaatattct
     6361 ttttctgtaa ctataggtat gtaatatatg tggaaaattt taattctctt aagtagaact
     6421 ctttttttaa caagtctttt aagtagaatt tttgtcgtag atttctgcca cttgtgtttt
     6481 tcattgcaaa tgcgaataat ttctttcctt gatattcttg aatttctttt ccaaaagatt
     6541 ttcattacaa ttaattcgcc acgttttaag ctttgtatta acatgatgca ctccttgtaa
     6601 atgtttataa tatagtagca tttttttatt taagatagta caggtaagtt attccagaaa
     6661 aaaagcaacg ctagaatgtt tcaaaactaa atatgataaa tatcatataa atgtcattaa
     6721 aaaatgattt atttttattg tgataagtta aataaaaaga attagaagat aataaaattt
     6781 tctagtcttt ttatttattt aatcttagat ttaacagatg cgtaattgca acgtataaga
     6841 ttattaaaag aaatagaaaa ataaagaaag caaaagatga tagttttttt atctattatc
     6901 ctttgctttc tctaatatta ggattttact atgtttttgt caattgcatt gtttctgtat
     6961 gctctttcaa ttctattttt accttgaaga aaataatcgc tatctaattc tattccaaca
     7021 aactttctat ttatatttgc acaagctatt cctacactgc cagaaccaaa acaattatcc
     7081 aaaacaagtt cattttcgtt tgtataagtt tttataagat attctaaaag atctactggc
     7141 ttttgagttg gatggatgca atttgtttct ttattaaaat acaatgtatt attaggataa
     7201 tttgtatatt cttgaacata ttcttttgat aaagtatgat gtctatatat tccatcttta
     7261 tttttatttt tctttttaca tgtaataggt ttatctaatt tttctaagtt ttgaggattg
     7321 tataaaggag ctttttatag aatacattaa tatcttctac ttttcgaagt ggctggtatt
     7381 tagaaaatgc gaatcctgta gatttatttt taatccagta ccaactgtat ttatagtttt
     7441 taatatttga atttatcaaa ctagtagtaa atggttgtgc tgaaaatagt actatagctc
     7501 caccatcttt tataattcta ttatattgat tccagattga tttgaaaggt attattgtgt
     7561 cccatttaca attagttgtg ccataaggaa gatcacatag aattaaatct attgatttat
     7621 cttctattag atccattatt tctaaacagt ttccattgta tagtttatat ttttctatgt
     7681 taagcatgtt gcaagtcctc ctaagttgaa taaattttat attgattgga tattacaaca
     7741 agttattatt tgataaaatc taatgctttg taaagtgtat caaatctatc attacctttt
     7801 atcattgtgt aatgctcttt agtaatagaa cctatcttct cacatgctcc accgcctaca
     7861 acataaagat tttgcgtctg acctggtacg taatctttta tatcacatat cagtattttc
     7921 ccatcattat aaccccaacc aactacagtt gcagggattt tgtcaacttc tccatcataa
     7981 actattgtat gtttgtacat ctgtttaact ccctcattat ttatattttt atttaatata
     8041 ccttctacaa ttaacttagc aatcccttca tgacctagtt tcttagcttt ctcataatct
     8101 tctttattat cacaaaagaa actttcaatt aatactgctg taggctttga actatttaag
     8161 atatataaac ttttatctaa tttagcacct ctattaccta cttctttatc tccttttttt
     8221 cttatgaaag gtttagacag tttatctact accctctgcg catattcctt ccctttttca
     8281 ctatagtaaa atacttctgt tccaaaagct cctacaccac ttgaatttaa atgcaactct
     8341 ataagtaagt tatagtttcc actattaatc ttaggtattt tatatgactt ttcttctgat
     8401 ttagttttaa attgcttttc gggacatatt attacatcta ccttatgccc ttcttttcta
     8461 aatatatctg ctaatactgg tgcaagagat ttgttgtatt gatactcgtt aattactcca
     8521 tcagcagaag tgcatgctcc actttttaaa atactgtgtc ctactgtaat acatattttc
     8581 attatttatt acctccctta atatttaatt cgtctgtcat tgtatctagt aaattgccta
     8641 ttttctcctt taattttttg ggaactggta atccacataa gtacatattt tttaatatac
     8701 ttacactttc atacaaaatg aatagaatag agaaaaattc agatattcca agatgattta
     8761 atctcaagaa atcaatccaa tcttgtggca acataaataa aaagttaaac tttgtaagta
     8821 tatcaactac tgctagaaaa aatatacatg ctatcattgc aacttttctt attcctccat
     8881 ttattccaaa acttgaatta aactgatgtg tttttattgc tcttaagcaa cctaacagtg
     8941 tatcaaatgc tattgctaat attactaatt ttataaatat atttgttgct aaaaaaacta
     9001 ttgttaaatt catatttcct cctaattttt gtattaaaaa ggacctagaa ttatctaagt
     9061 cctttaaatg tattaattta ttattttacc tttaaattgt taacgaaatc tgcagctata
     9121 ataaagtatg ctgcatcaca ttttgcttct cccataatcc ctggattaga aatgtaacaa
     9181 cttgttgggt tttgttctag cccctctagc tttatatttt ctaccattat aaatatctcc
     9241 cccatagtag gtataattaa cattagaact agaatgcaaa ttacaattat tttcttcatt
     9301 ttttaatctc cttttaatag aattttttta ttatagataa aaataaaaaa tatataacta
     9361 aaaaaggaaa ttatttttaa tttatataaa aaaaacctat tttgttggtt ctgctccttc
     9421 tactactcca ctttctctaa ttatataatc ctctactgct tttctatact ctgtgttagt
     9481 tacgtcatct aattcaaatt ctcgattttt tagagggttt aatcctccgt ttaatatcct
     9541 ctctgctaat attcttacta caacattatt tatattcatt ataataatcc tcctactttt
     9601 tcattttcat ttaataaaac ttgattttct aattcttgta ttctcttttt ttcttcactt
     9661 acatatactg gtatttcttc caaaattggt tgttttgttt ctatatttat gcctataatt
     9721 ctatttttaa tataatctat acttccatat ggaatatcaa tataatctaa ttcagttatt
     9781 ttattgtgtg gtaacacatc cccacaggac tcacctgttt caaaaattat tttgcctgtt
     9841 tggtcatata ttactctgtt tggaacattc atattttttt ctccttctta aataaattta
     9901 atagcatacc atttataaat atgtctttga gttgagtcaa agttataagc tggaatttga
     9961 acaccagtat tattaaaata aacatcccct tcggaattat tatagagcca accattagca
    10021 tgaaaatttt catcactgtt ttttctaata aaagctgtaa cgcttgaaaa atcaacacct
    10081 ctttgtgtag cgacaccacg acaagcaaaa aggaaaagtt tataattatt tttatattca
    10141 gtatcatagt aatcaaaatc agcaaagaaa atgttaggct taaaatctaa attactaatt
    10201 ttaacccaag taccaggttt aaaagttctg tcggttagat atggataagc aattttagca
    10261 ctatccgcaa aagaagtgac tgttccacta gcaactttat atttagagtt taattgcgat
    10321 atagtattat tagcttgtgt taactgattc atcaaatcct gtaaactagc gtctccacta
    10381 tcaaaagact gctttatttt ctctgataac tcaacaagtg tgttattcaa acttgcttct
    10441 atattcttta atgctaaagt atttataata cttgttttac catttctaaa cccttctcca
    10501 atctctatca acttagttga tatatcactt aaactagcat cagattgaag tggcattatc
    10561 tctttgctta tacttagcac tttttctgct gttgcatttt ctgaatctgt agcaactatt
    10621 tttaatgtgt gtattgcatt gtctgcaagt tcatagttta ttgttttctc tgttgttaaa
    10681 tctgttgtta ttgtttcctt taatacatca tctataaacc actctatttt agataaatta
    10741 ttatctgtat ctattgctgt aaatgttgca gaagtggagt tataagaagt tatatttaat
    10801 tttggtttag tattaccttt tgtaaatgta acagttttat ctaagaatat tccaccataa
    10861 ccattatcga gttcaatagt tatattattt acagagttaa atgccaactc agataagagt
    10921 tcatctgtaa taattaattc atagtttcca tcaacaacat tatttttttc agctatttta
    10981 acaccattta ttttttcgat tattttaaat ctaacactag aatcactatc tgatattcta
    11041 taatttatac tacacgcatt accaatcaca cctaaatcgt tacttataaa aatagtaggg
    11101 tcgggtacaa actgaacagt tgtttcttga gtaaaattcc tactaatatc aacttctctg
    11161 ccatttatta tctgacaagc cctagttgaa taagctacaa attttttagt tgcagatata
    11221 gaaactcttt catattggtc taaagtataa taagcaacaa agtcagtata ataaggactt
    11281 ccgtcgttat aagtaagacg tgaagcatga atatctgtca tacgataata accacgtctt
    11341 cttttgacag ttctttctaa aacagttgta gacatactaa tttcaccttc ctttctacat
    11401 tggtatcaaa ttattattta ctgttgatat aatactactt ctattacctt ttacttcttg
    11461 catcacttct tttaatgctc cctctacatt atcacttgaa aataagttat ctgtatcttt
    11521 tatacttgtt ttctctgctg ttgtttctat gctgtctaca ctgcttttta cctcatttaa
    11581 tgcactaact atatttgttt tatctgttgt agtaagttgt gttgtatccc ctattttagt
    11641 gtttaactct gttttagtag tttctatgtt gcttgttaat tctgttttag ttgtatcaat
    11701 tttagcgtct aaatcttgta tatctcttaa tgttgctagc acaacagttg ggtctatttt
    11761 taaatttata cttgctgtat tagacacagc taatataatt ttaattaata tttcttttac
    11821 tgttcccgaa tctggagcag gtttataagt ttcgggatag ctagatactg cgagaagttg
    11881 gtctttagag tcgaatactc caatctccct aattgtaaag cttccagtat ctccagttat
    11941 agttttttgg ataactaccc aattaggatt tttttcgtct gcttgaacat gctctaatgt
    12001 gctttcccac actacatttt tgagttctgt ttgagtttct gttggaatat atgcacttcc
    12061 accaccatcc ccaactttta tttttgcaaa atccactcta gttccaagtg ctgtagcgtt
    12121 tgctatagcc gccttgccta tttctgtaag taatgtataa tattgtgctt gtgccaatta
    12181 tatcacctcc tgtgggtata atgttaccct ttcagcactt ttattgtttc cactagctag
    12241 tattatttct ccaaaacatt ctatattttt aggtgtataa gggtatatag taactgtttc
    12301 tcctgcactg ctcattgctc ctgcataaag tccattttca ttaaataaaa tcatttcaaa
    12361 tttatgttca aggtgtgcag gttttatttc ttctattttc ttatctaact ctaaaatagt
    12421 gttataacta caatcatttg ttataaaact aagtgtaaaa ctaaatagat tactaaatac
    12481 ttctacatca acattggtct ttgtgtaagc ttccgagata gcttttataa cctctattgt
    12541 tgttgtaccc ttacctctca tctttgcttt tatattgctt cttctagtat cgaagtctaa
    12601 cttataattt acttttatgc ttaaaaggtt ctcccaatca tcaagtcccc aagttgctgt
    12661 atctataaaa aactgttcta ataaatcatc tttttcatca attagcgtta aaagttcatt
    12721 ttcaagtgct tcttggattt gtatgtcaat ttcattgttt gcaaaacttg gcaggtaatc
    12781 aattaacttc aatttaactc acctcgatat caaatatagc acttgaaaca gcaggaactt
    12841 tttcttcatc aactattatg tttttaacat catcatttac aaggagattt tttatatcat
    12901 gaacaccttc tatgcttgct aacaaactca ttatttttat ataaataatc tctcttgaat
    12961 tttctattaa ataagaattt ataatatcta aaaacacaac ttttatagat tcaatatcat
    13021 atccattttc aagtgttaaa gttgcactaa tattaatatc aaaagtactt ggtgtaacaa
    13081 ttgtaacagt aggtccaata ggtttttctt cttctatatg ttcaatacat ctttgcaata
    13141 cttcattatc aactgattga ttattttgac cataaattag aatcttaatt gtacctggtc
    13201 catcccaacg aggaattact tttgcgttat aaacaccttc aacctctaaa gcccaagact
    13261 ggtaatgtgc tttatttcca cttgtcgctt gatttttttg tattttatag aacctttctt
    13321 ttaattcttc atcagtttct atttctgttc caccttttag gtcaagttca ttataaattt
    13381 tagttactcc atttatttct tctataagtt taaattctgt gttagctgat aaattatact
    13441 taatgccaac ctctaaagcc tgtataaggc ttacatttaa cttactatct tcatctattg
    13501 taacatcttt aataactaca aatagtaaat cactatgaga tactattgtt ccatttggta
    13561 ttttagttcc tattttgccc tcaaatatta cctcacctat tgcttctgtg cctaactttc
    13621 tatatacacc aaattcattg actcttctat caagaaaatc gtcaaagtta tcttgaataa
    13681 aaactctctt atgtatataa gaaagttcta tatataattg tgcaagttct gaatttattg
    13741 gagaaactat attataaaga gaagatcctt ggcctttata aagagggaga tttatgttat
    13801 ttaaagttct attatttaag gtactaaatg attggccact atacaaaagt aatctcctcc
    13861 tctccataaa ttgtttttac atttaaactt atagataaat tatcatcttc aaattctgca
    13921 tttgttatat tgacttctaa tatatatgga ttagttaata gagcttcttg tatatacctt
    13981 tttgcctcac tttcagtaag acctttagta tacttttgcc ctattagatt ttttatatct
    14041 gttccataac tccaatcata tattaaatga acatacttat ctgtttttat tgttttgtaa
    14101 atccatactt taattgcttc atttctttca actattttaa agtctccatt ttcaattatc
    14161 ttttcatctt tattaaaatc ccaagcaaat tctttaaaaa taggtaattc ttcattgtct
    14221 ggagaaatat aatcttctgg aacacccatg aaagggaata tagtattatt catctagact
    14281 caccaattta cttacaacag caaatttttc acctatttta aacattatta ctgtgtttcc
    14341 agattcaaaa gtatctataa agggattttt tatttcatgt ttatgttctt gacttgtttc
    14401 tgtatcaaat aactctatct gtctatcaag catccaacta tctatcaaga tatcttcttt
    14461 ttctaatatg atgttattta tctctatttt taaatctggt aatttacttt taatttttcc
    14521 aataaaaaaa gaaggttcat tatgaaattt accttcttgc ctaattattc ctataaattc
    14581 attgattgga tctgccacga tatcacctct tttacaaaac tcttctagct gtattaaaat
    14641 cttttctatt acttaattta cttattttaa ctacatctcc tgtttgtggc gcatgtaaaa
    14701 attctccatt acctacgtat agtccaacat ggctgactgg attatggaaa aataccaaat
    14761 ctcctgcctg taaattattt ttctctactt tcttacctac cttagattgc tgacttgagg
    14821 ttcttggtaa attaacatta accttcttaa agcaatatac cattagtcca gaacaatcaa
    14881 agctacttgg tccattccca ccccatttgt atggctttcc aagatgtttt cttgcttctg
    14941 aaattactgt ttttgctttt tctgtcatgt ttccagaata accaccaatt ataatctttc
    15001 cttttcttct tccaaagttg ttagcttctt caacatttcc aaataaaata tctatatgat
    15061 atgttccatc cttttctata attattgcag gcccattatc atttacttta tacgttccat
    15121 ctcgacttga aacacctgta actaattgta tttcatctcc atatctcata agaggatgtt
    15181 tgtttaagaa atcttttgta tagtaagatt tctcatatga tccaaccata ggagcagcac
    15241 atgttttttt agaaggatct agttttttac ctctacaatc tttatctcca ccttcagatt
    15301 ttctaggaca ataagcagta aattcagcag agtattctgt tcctccagta tactctgaac
    15361 catcttcttt ttgttcatct tgaccagcag atttttcatc cattatattc tcaaaattaa
    15421 gctctagttc gatttgataa tctccatttt gccatgtatg cttatctgta tctatataaa
    15481 aaagaccaat taattttgta tatgagtctt ttacttttac acctctacca gttacacaag
    15541 ttatatcacc atatcctttt agagagcaag ttttttcgat tcctttaaac tctccatcta
    15601 tgtctatagt actgttttct tgttgttgta ttaccttttg cataattact ccaacgtctt
    15661 taaatataga gtcatttatt ttctcactaa tcttattacc atactgatca acaaccagta
    15721 ctttattttt tacattctcc atgctttcag aaaaatttgt attgataaga ttaaagcctt
    15781 cttcaaatgt tatatttaaa gtaactacac ccttctcaat gacattaaat ttatcaagat
    15841 tagactctat catatacttt tttttagttg ttttgcttgc ttctgtataa acactcatta
    15901 tagtatcata accagttacg ccaataaaca tttttgtata tttaactcca gtcttaggta
    15961 aattacctag tgggagttta ttttctaaaa atacttgttt tgcaatatct tcaaccaatt
    16021 tatctttaaa attgtatgac acttcgcttt gtaaaagtaa aaaacccata tctttagctg
    16081 taaaatctat attattattg ctagagtctt tagatctatc tattatcatt cctctaaaaa
    16141 gttctttatc atctacataa aaacaaactg tgcttgctac tggaatatct atctgcctaa
    16201 aattaatatc acttgcagat tgaattatag aaaattctaa agttcttgat ggcgatctat
    16261 aatcaccaga ccaagtgcat ttatccacta aatcagttac attatatata tttccgtttt
    16321 ttatatgaac ttgtattttg attttattaa ttataaatca ccaccatttt taaggaatta
    16381 ttaataccca accatttttt attacagatg gatctttaat ctcatcttca tttgctttat
    16441 aaatcttctc ccataaatct ccattgccat aatatttttt agctatctta aaaagagtat
    16501 ctccttcaac aaccttatgc gttttttgct tagtatcaaa tccttttgtt agaggaacat
    16561 tcttttcaga agataatttt tcatcattat taatgtttac tttggatatt tgtatccttc
    16621 tgtattcttt tagacttaag gtaaaataaa tatctcttga ataatcttgt tctctataat
    16681 taaaatctgc aattatacat tcaaagttta tattagtttc tgtaatgata aatcttaata
    16741 tatatccttc tttcatccaa ctttctatta aatttacaca atcatatggt tttggaaatc
    16801 cattataatt acagaaacta tattcttgat tagggaaaaa agaagatagt tctatagttt
    16861 taagccctaa tcctccaaat actgcgatat ctccaacact taaaatatta gatgtattta
    16921 ttgtcgcact tccatttatc tcaaaagagg gtggaataac aggaaaccta aatgtattat
    16981 ttgcttgtct taaccatatt tccattaaac tcctcctaaa aaagacactt aagtaaataa
    17041 gtgtcttttt aaatatcatt tactatattt aaataagaaa ttaaatttat aatacccctc
    17101 cagcatttgc gatagatatt tttttattta ttttctttac tatcttatct atgtcagctt
    17161 cttctcttac aattattgta tcagccaatt tatcaagaaa taaacttcca tttgaactat
    17221 ttcttttgta ttgattagct tcttgtttgg ttaaaagttt ctctccttca tgtgccctaa
    17281 ttaaataatc atttctagga actctattta taccaaatgc ttttcttgga cttttgccag
    17341 cagcatctgc gccttttcct atttgtaaac taggaccact ttttactatc tcgatcattc
    17401 ctttgattgg attatgaaaa aattctttaa gtttattcca ggcttctttt acgcctttaa
    17461 ctttatcttt gaaaagagaa tcagcaagat ctattacagg ttctagtacg ccactaacta
    17521 gatcaacaag gccattccaa attgatttta ttacacctac tccactatca aatatttgtt
    17581 taagtccatc catagtttga tcagcatttc ctgttataag ccccataata acattaataa
    17641 ttccagatat aaaagacata attccattta tgattccaga tactgttgtt attattgcgg
    17701 ttaatgtatt taaaacccat gtaaatgcta aaattattcc gccaacaaca ccagctattg
    17761 caattcctat agatggtaaa agttgttgtc ctatttgtga taggaaaggc ataaatcctt
    17821 gaaaccaagc tttaacagga gcaagtgctt gagttaaact ttggaaagaa agtttaattc
    17881 catcagtaga ctttttaaca ctatccattg gttggcttac tttgtttact gtatctccaa
    17941 cctgtttggc tggagtaaaa aaatcagtaa tcgctttttt aaccttatca aaacactctt
    18001 tcaaattatc taaatgaggt tttaaaggtt caaatgcttt tactaaatta tctatatttg
    18061 ttttaatatt tccagctaat atgttcttta attcattaaa tttatccttt attgaagtta
    18121 ttgactcctt aaacttatcc ttaaaattta ctccagaatc ctctaggacc tttaatgcag
    18181 gtttaacatc atttaatatt ttgttttgca gttctgtaaa tttagttttt ccagattcaa
    18241 ctccttgact tatagaatct gtcataggtt taataaaagt tttaaaatca ctaaatgaac
    18301 cttttagatt tgctattgtt tgttttaatc cacctgtatt tggtgtagat ttcccagctg
    18361 catcagctgc tttacttcct ggaatgttta catttgtttt agtagatatt ttggcagttg
    18421 cttcaattgg attttctaag aatgttctta aatctttcca cttatctgta agccatttag
    18481 ctttatcttt aaataagctg tcagctatat cagctatagc ttgtatagga gcagttacaa
    18541 aatctatgaa acctttccaa attgatttta taatttttac tccaccatca aatatttgtt
    18601 taactccatt catcatcttt ttactatcgc ctgtgacaat acctataatt aaattaaata
    18661 ctccatttaa aatagatgct agaccattta atacattagt tatagtatca actacagctt
    18721 taaaggcagt tgccagagta gataaaatca aaactaattt tgctatatga aaacctatta
    18781 cgccaattaa aacaggtcct agtaccttca ttattacact tcctaagttt aaaatagacg
    18841 caaatagtgg accaaatgct gataacaact ctttaaattt ttcccctaat tgtttaagtg
    18901 caggcataca aaaatttact ataggttttg caaattctgt aaatgatttt actaatgaat
    18961 taaccttatt tctaaatact tcagatttag cataagcaat agtaaatgct actgccaatg
    19021 caattacagc tgttatagtc catcctacag gacccattac agctaaaaat actgcactta
    19081 atggtttcat gcctattaca aaagctctga aagcaacttt tgctcttaat attattggaa
    19141 caaaaaagcc aaatacacct atagcttttg aaacaattaa agacacagcc cctatagtta
    19201 ataaaagtac tccaaatgct ccaactgaaa gcattacatt agttatgact ctttttattg
    19261 gttcacttag attattaaac caaacagtca ttttagttaa attatcaact acagatgcta
    19321 ttgctggctt tagttgatag taaatagtaa ttccagtttc ttcaagggct gattttaagg
    19381 ttgcaagtcc acccttagta ttatccttca ttacatcaga cattgttttt aaagcacctt
    19441 ttgaattatc tatttttttc tttaaactat catattcatt tcccacacca tcaagtaact
    19501 tttgtaacgt ttttaattga gtctttccac caatagcaga taagtaataa tttttctgct
    19561 catcagtcat acctttagtt ttatttgcaa cctctttaag tacatttgac atacctttaa
    19621 atttaccttg attatcaaat gcacttacac ctaacttttt cattgcctta cctgcttgac
    19681 ctgcacctgt tgtaagattt accatgatag agtttaaagc atgacctgca tctgtaccct
    19741 taactcctct atttgcaagt attcccatga tagcagttgc ttctgaaagt ggaactttta
    19801 aattgctaaa tgtaccacct gcaacattaa acgcttccat caactgttgt atacttgtat
    19861 ttgatttagt agatgcattt gcaactttat ctaaatactc acttaaacct gcaccagcta
    19921 gtacttttcc acttttactg accttatcaa cttctaagca catggaactc atgctatcag
    19981 ttactaaatc tgatgctaga ccaagatcca tagaggctgc ttctgataaa ttaagcactg
    20041 gcattaaggc atgcattgac ttttgaacat cccatccagc aagccccata tactgaagtg
    20101 cattagcaga atcagcagca gttttagttg tagcttttcc agcatctcta gcagccttag
    20161 acagagcttc aaaatctcct ggttttaagg aatttccaaa tgtagcctta acttgagaca
    20221 tagcgctttc aaattctgtt ccaactttag ttgcagctgc aaatgcgcca ccaactccag
    20281 cagtgataag acctcctgct tttaaggcgc tagttccaac actttttaaa ttactttgaa
    20341 cattatttaa tgctcttgat gcttgggaag atgaatcatg tagagcccta gtatttgctg
    20401 ctactctttt taatgttgaa cttgcttcat ctctcatttt tattattgct tgtaggacct
    20461 ttgcattaga actcatatac taaaacaacc tcccagcacc agattttatt tcttcttttc
    20521 tttcatccaa ttcttgatcc ataaatacac taacaatttc tttttcgcct cttagtatac
    20581 gataagaaac ggatggcatt atacctttat acttaaataa taagtacatc aagttaactt
    20641 ctccatccgt tttaattagt tttttatatc tttagctctt tctttttgtc tttttttatc
    20701 tccttcagca tctatgccat ttactttatt aacttcctca tataaattat caatttctcc
    20761 cgctaagagt aattttttta gaaggtcttt gggggttgcc aagcccagat tttctacaac
    20821 ttccatattt ctaaacattg gacatgactc aagtattgta tttaccttaa gttcataaac
    20881 attaatattt tctaaattac cactatctac atccattgaa ttcattctta tctcatcaaa
    20941 acgctctgga tcaatagcat tacattcaat ttctaagata tcatctaatt ttttgcaata
    21001 taattcaaat atactagacg gcattttcaa tttacctgca tccatattta ataatttttc
    21061 tactgtattc atataataaa aatcctccta tttttctgta attttatcta aatatttaaa
    21121 gctttcaaaa gtaaatggaa cttctatttc tcccattttt cctgcttccc aatctgctaa
    21181 agttaagtca tcaagcatac aaccataaag agcaactctt tcagctccat atgaatctgg
    21241 gtcttgtaat ttacttatta tagtaaattt gaatgctttt ccttcgttta acatactttc
    21301 aacatattct ccaaataggg aactcacttt aaataaattt acagagccct ttccagatgc
    21361 tccaatgagt ttttgtccaa ccatcaattg tccacaaact ttaacctctt ctttttctaa
    21421 ttcaactttt gcctctaagc cttttacagc catcatttgc ctaccatcta tccaacattc
    21481 tccaaatgta ccagatatta ttctacttgg gtctatttta gctttcaaat tttaacctcc
    21541 aaactacata gagatgtcta aatacacatc ttccatggca tctattaatt taatttttgc
    21601 ttttaaaaat acaattgtat ctgtgttata ttcttttatt tgttgttctt ccatattaga
    21661 tacatcaata tgattttctt ttaaccatgc tctttgagca tctaaattaa tttctattgt
    21721 tgaacctggg tcaattaaac catccttttc taattcttga agatataatt gtattgcaac
    21781 aataagtaaa catttattat cataagtgtt ttgggttttt cctatataat tttttacaat
    21841 tattttttta atatcgttat gaatttgatc tagtgtatca accaacttaa tctttttgaa
    21901 tatatcacct tttgaatcag taattgttgt aaaagatgta actcctctag cgatcactat
    21961 agctccgctt tcttttatta aaattagctc tccattattt atccttgtat ttgcttcagc
    22021 tcttgttaat ttaggtatat tagtgacttc tggtatttct gcatatgtta cagattgagt
    22081 tgatggagtt cctgcaataa aacctgctat tcttggtaga aattcatttg aagtgtattt
    22141 tttaccttca acttcaatgt cagttgctgt aaagtttatt ataccttcat aatctgctga
    22201 attacttgca gtaatagctt taacctttat tttattatct tctctcattt tcttaatcca
    22261 cgttttgatt ttaggtaaat ctccttcttc ttcatctggc atacaaagat aattaaattc
    22321 acaagtttct agaaaatcta gtgcatcatc tattgattca tctgtatcta atgtataaac
    22381 taatacttta tttggaggat atacatttcc cttcatagcc atttttatat agtctaaatt
    22441 agtggcagaa taattttctg gaatgtcctc catttctttt atctcaacta gccctatatt
    22501 ttttgtatct tttaaaataa gtgctacaat acctccagaa cgtttaacaa aagtttttcc
    22561 tgcttggata aaagaaatat taatttctgg taaacccatt taatcacttc ctatctttaa
    22621 atctacattt tccatagtta catatgtttc tttactaaag taaacttctt caaaataaga
    22681 tatagtcatt agaaaagtta gtttatgccc cacagaatct tttacaattg atggagaaat
    22741 attttctata gttaaactcc tatctttaac tctaatgttt ctattaaata ttttttctaa
    22801 tctactttgt atgtcatata gatttaattt attttttcta gctttttggt aatattcaat
    22861 atctatgaat atctttttag tattcaaaat tttatttgca atattgttag aaacaggtaa
    22921 aagttgcaca aaaaaacaag cctcctcaaa gccttgtata ttatcctcat taataaaaat
    22981 atctatatct atgaactcat tttttaatat ttcattaact gctattagaa tgtctctgtt
    23041 tgtaagcaac tcaccaccta ccaatcaaat aaatttgctt ttgcacttgt catattttct
    23101 tttgtttttt caattccctt ttcaaccata aaagaaccag gaataatgcc accattttta
    23161 gttgcatggc catcatttac taaagttgca tattcaacct tgtcttcaac aatataagta
    23221 tcatattctg gctgactttc ttcccatcca ttttttaact tttcagttct cacaggcgtt
    23281 tcatctctta tattttctgc tagttcttgc ccagtttctt ttgtaaactt cttgattttt
    23341 ctattaaagt ttgatgcatt tagacctaaa tctaaggcta atgcttcaaa gactcctaaa
    23401 ttatctattc ccattatatc ctctccaatt ctgtaatagg tatttccata tggcttggat
    23461 atatatttgc cttagaaacc ttaaatacag cttcttctcc atgattatag gttgtattta
    23521 tagtatcacc tatttttata tctacttctg gtctacaaaa taactttcta tttacaacta
    23581 tagatccaat atcaccattt acaatggctt cttcttttct agaaattgca catgggatat
    23641 tttcagctat aacaacatta ctaaaatcat tagaacatgt ttcttcattc cagcctttta
    23701 attttctaat tatagtcatt ttatcaaggt atgtcatttc taatatatct gcttcagtca
    23761 tttaatcacc ccttagtaca tactaacaat acaaaatggg cttagaaatt ttttatcagt
    23821 atcagttaaa aaagatgatg aactgatgct attttcagca gtttgtaatg ttaaagcatt
    23881 atattctatc ttagtgtcac ctctagttat agcttttaag cttttttcta gattatcaag
    23941 tccactattt ttcttaagtt cttcaaatct tgaagataat attgatatta ctttttcttc
    24001 tacaaaccct tctagtgcta tacttagttc ctgcctagaa caatattcta aaaccatctc
    24061 acttatttta tttatataaa ggattattaa tttgtcatat ctatcatttt ctattcctaa
    24121 aactaattta acattatcaa gcattgtact catctagtag ttgaattaat tcctctttag
    24181 ttttagttga atatcccttt atattcttgc ctttagcaat ttccataagc tctggtttag
    24241 atagattaaa taaatcatta gaggttgatt taactttttc ttcttttttc tcaatattct
    24301 ctttagtcac ttcttctgaa tatattttaa atccttttga aatcaattta tctctcgaat
    24361 tttcatcttc aacaacttta attatgttat tttttatcaa tgcaaacatt taaaacaact
    24421 ccttattctt gtgcagcttt agcttgtctc acagatgctc tacaaagttt taaatattca
    24481 tccaatatcc acaaatcgtg atattttcta tagtctattt gataagcatc tgctgtttgg
    24541 ttttgatctg gagtaaatat tcttggctta tcagtcttag atactgcaat tggagcagat
    24601 gctggagtta ttaaccagtt tatatctaaa gcctttgctc cttcagtaaa cccaccctct
    24661 gtttgtccag atgttttacc atctctaaat acgtattctg ttttcattct ttttgatgga
    24721 acttttatta taggtacatt gtctatagac tttacaattc tttttatatc tccatcgcca
    24781 aattcaatat catcaagttg agatgattgt tctaatatgc ttgcaattat gctagacata
    24841 ctaataataa gaggtatatc tccagataaa tcttgtattg atgcaagttg atttttcaat
    24901 tcacttaata ttgtagttac tgtagcttcg tatgaatatt ttacacgctc atctacttta
    24961 gctgtttcag aaaatatctt tgagtatcta tatgcatcta tttctggtat aacatgttgc
    25021 ttttgaaata atcccattac agaagatgca tttataacga agttactttc atttacatcc
    25081 attgaatcta acataaaagt cctacctcta tccatctcca ttttcattgt ttcgtactca
    25141 agtgtaactg cgccttgcgt atatccttta gatctgtcat aatcacctaa gccatccatt
    25201 gttacttttg gtacttttac ctctcttcct ccattatata taacttgacc agcatttttc
    25261 tccatccatc cagtagttga gccaactgtt acctgcatat ctagttcttg ttgaaaaagt
    25321 gttacatatt gtaatacatt agccatttat aaaatcatcc tttctttttt tatactaaag
    25381 actatttagc ccttgcttta tttgttttct aatttctgat agcgaacctg cactatcttt
    25441 gccaggtgga acgtatgaac catctttaat tctttcatct acctttgact gcacatattg
    25501 cttcattgaa ttttcaaata tctctatatt tgcatttgtt ttatcatcat catcagataa
    25561 tagatattca attaagtttg taggtatttt cttttcaact aaaatatctt tatatttaga
    25621 aactttttct gcgtactgct tttctttttt tagtttgtct aggctttctc ttaattccct
    25681 tattgctttt tgctcttctg tttcatttgc tccagttctt tttagaactt cagcacttat
    25741 aagtgtctgc atatcttttt tcttaaagtt ttcaagtgct tcgctgtgat atttattttt
    25801 ttcattatct ataatagctt taaagtttgc atcattatta attaattctc taaactcttc
    25861 tattgtaagc tttttatcag atgcagattt gaataaatct gttgattgca gcaatgaatc
    25921 tatttctgca tcatcttcta tttcttttag taattccaac acttcttttt ttaacatgta
    25981 taaagttctc ctttttgaaa atttaattca aacattctat tttatccata acctttacta
    26041 ttttaggaaa ttgaattgca aagaagtcaa tcatttcttc gtttgtagcc caagaactac
    26101 aactcttcaa tccactttca ctaagaaatg catgaattat ttcatgccta gcaacttcat
    26161 ttttatatac acttaaatct ttcaatgaac cttcttcaga atttattttc tctataacaa
    26221 tttccttaac agagtgatca caatatccag ctctttcctt taatagcaca tcttcgcttt
    26281 caagtttctc tatcactaga tattctgttc ctaatatttc aactttcata tttcacctca
    26341 tattttttag acataataaa aacacctatt aaattaatta atacatgtta aacaggtata
    26401 tattgacatc tacagtgaca gtgtcttggc aaagagggcc tatcttcatt gacataaaat
    26461 atcactccat tatgcataga acaatcacta catgtgcgag aatctaaatc tgcacaatac
    26521 attactttta taatcccttg aaccttgaaa aaatactcat ttacttcact ttgacatctg
    26581 gcaacttcat tttctattag tctgctggtt tgtcctgcac ctgttgaata tttatctctt
    26641 attgcttttt ctatcttatt tacagaagtt tttccattta gaaaactctt tatttctttt
    26701 tttaaggact cttctatacc ttttttattc ttccagagtc tatcgctcca atgttttcca
    26761 tcaaccttat tattcactat cttatttata actttattgt ttatattact aacatctttt
    26821 atcttcagta ttactcctaa taaaattatc aaatttgaat atttattctt agatgaatca
    26881 gataaaatag tttctatatc cttgacttct tcatcataac tattagaata ctcatttata
    26941 attttattaa gttttgacct tagatttttc ttatctttat tattaatatt taagtattca
    27001 ttaataatat cgtgtgttag caatatcttt gctatttcta ataaaagttt atccctattt
    27061 tctctttgat acttataaaa tggttttgtc ctattttcag cagatttata tatatcttca
    27121 gtcaatttag ttataatctt ttgattcatc taataaaatc tctccctctg aaactatttc
    27181 ctgttctttt ttagcttttt ctatttcagc atgaggatta tcaatgaaac ttagttgaga
    27241 taagccagtt tcagtagata atttatctcc aagttggctt aatatatttg ctacaactaa
    27301 atcatcacgt ggtatcttag gggtaaattt agccttaata tctttataat catagtcaaa
    27361 accatatttt ttattaagcc aaatgcacaa taatttgtac ctattaaata aagtatctgc
    27421 aatactatca ttattttggc tacaaatcca ttctgtactg attaatcttg ctataactgc
    27481 tagacttgat gtattacttt gcattttttc attgtgattt atatgagatg taatttcata
    27541 catattttct tttattgaag atagtgtatt ttgaacaaat gtatcattca tatctttaat
    27601 aagccactct atttttccat ctccattaac ttgaagtatt ccaagttctt tcatccttgg
    27661 caaatcatct tctttaatat tacatccaga aaaggttaaa taagcatttc taaaatcact
    27721 aatttcatta cttatatcac ttaaattagt ttcataagca tcttgtaacc cttttatatc
    27781 attaaatatt gtattttttc ctagttcttc acttcttttg caaattgata ttggaacatt
    27841 tccatcaaat atattgttta ctgtaggact ttcaacttct ttgaaatttg aatcaaaatg
    27901 atatatatat tctttgtcat atacatctac atacgtgtca tcttcgaaac ctttggtaaa
    27961 ctctctaata aagcatacta tttctccatt ttcattttca taatgacagc catctgttgg
    28021 cttgattatc tttacttgta tttcatcatc ttttacatag taaagttcat agcaaagccc
    28081 aaataatagt gcatacctca ataaatctga gtcatgtttt ttactccaat ggcaagtata
    28141 gtattctaaa tcatttataa tcttctcatc accagatcta cttgtatagg taatcttatt
    28201 tgcaagagaa taagcgactt cttcattaat aaactttttt aagaaattag tgtttatctt
    28261 attattagat ctcttggtaa ccattttata gttgctaata gcatccgttt cacctttata
    28321 atacttatac atagtatcat attcggattt attagagttc caatcgtcat atatgtcttt
    28381 tattaagtct aaatctaatt ccattaacaa cacatcctat cctgtagact ctataaattt
    28441 taaaactttt attggaatat cttgctttga aatataactt ttactccttg ctctatgagt
    28501 atatgcaaaa tatccttttt catctttttc tattccaaca ttgaacttct ttcctttttt
    28561 agttaaagtt ttttgaactt cttcaaactt tgatctatta gccaataaat tacctcccaa
    28621 ctaaaaacct aataattttc tatcaaagat atttactgtc tcagaaactt ttatgttgtt
    28681 aattcgatta ataaattccg atgatatatc cggagcatca tcatgtagag agtatttttg
    28741 tcctgtaaaa tcaagtattt gctgtataaa ttccttatct tcttctgcaa aaatcatttg
    28801 gcctttattt aaaacaggta ttaaagtaga gattttatca tctttatttt tcttttgttg
    28861 ctcattaatg atttctatat ctctataact aagaacatta tcatttttta ttttaaactc
    28921 taattggttg gcatctgcgc cattaaatgt atttttttct atataaatat gtgttatatc
    28981 tgaatacact tttaataaat aaatcatatg gtctatatat ttatcaaaat cagttctagc
    29041 atttatttta gctagttctg ccaatcttcc atatagtaaa ccattttctg attcacttcc
    29101 aacaagaaaa gcgctatagt catgtttcga cccacccgca gatgctggat cacataacag
    29161 cattgtcttt ttaaaagtat gcgattcaat ttcttctcta gtttctgttc taacagtttt
    29221 aaaccatttt tctcctatag aattgacatc attttgcact tcctgcttaa aagatgttgg
    29281 attttcataa taacttagag ccatatctaa acaatcccaa aaacttggcc ataatatagg
    29341 gaatttcatt tctttttcat tttcccagta aaattcttta gcatcttcaa gatgatttgt
    29401 atttttaaaa tcaaataaga tctctttgaa cttaagccat aaaccagaat taaataattc
    29461 atctacatta tcaactaaaa ctcctttttc gcatttaaac ttccaagttg gtaatttttt
    29521 tagtctacta taaaaacatt ctttgtgttg taaggttcct aatgctataa aagttgttcc
    29581 tctttttata gttttaccat ttcttatgac tggtttttga gcagcatatt ttacatcatc
    29641 agaaaatctt ttaaattttt tctctctagc atcttctgtt ctaacatttt cttctgactg
    29701 ataatcatca agtatgataa gatctggacg gttattattg tattttcttc ctctcatggg
    29761 tgatgaagat gatatagctt caataaaagt tttatttgtt agctctagtt gagtactatt
    29821 acaaatgtat tttctatcat tatcatttaa aaggacacca aatgcttttt taatatactc
    29881 attttcaagt aatgcatttt ttatatcttt tacaaatttc tctgctgtag atcctatgtc
    29941 agagcaaatc aaagtatatg tcttatgttt atagcaatgc gaccatacag ttgtcgctaa
    30001 atctccaaat gcacttttac ctgtccctct tggataaatt cttcctaatt gctgtgaacc
    30061 atctccaatt atagattctt gtatatcttc ccatagctca tgatgaactt tagctatagg
    30121 agcagcagca ttatcttctt ttggtaaata tgtgtcttgc ataaaataca tacagaaaaa
    30181 ttctaggttt atttttccaa gttgataagc gagaccatta actccccaaa gattgtttga
    30241 attggctttt ataatctcat ttgcttcttt ttcagcatct tttatagatg ctccatttct
    30301 tataaaagtc tttgctagat aactaaatat taattgtata tttctttcta gattatccac
    30361 tcgtatttac ttcctcttac ttcttctatt tttttaggct cagtgagtaa tgcaattaat
    30421 aaaataaaca atgtaaattt ttctctaata aaatcaccct ctttgaaata catctctggt
    30481 ttttaaattt tgctagaaaa tttctagaac tcattttcca taaaaaataa ttttttcaaa
    30541 atagaaggac accccctatt aataaaaaaa ataaatatat taaagtattt tgaattattt
    30601 tcttgcaaga aatatttttt tgattagaat tattaattat ctttcaacta ttccttaaat
    30661 atgtattttc ggcatagtta aaaagaacct agcatttcca ataggttcac atatcctata
    30721 ataatccaca tcacttcact gtatgaaact ttaattatta agtctcacta aattttatta
    30781 aattagctat aactaaagtt aaatacaata tatgatttaa ttattaaagt aaatatatat
    30841 ataatatatg ttttgcttta attgatttca aacataatta aaacatattc ttgcaggatt
    30901 tatttcatat ctaattactt cttgcttgtt cttattctta tcttagctat agctgcttct
    30961 atctctgttg catccatacc catgccaaca ctattatcct ctgcttgtat aactgtactt
    31021 gtaggagttc catatatacg atttataaga tactgattgg ctgccaacat aaccctctta
    31081 tcgctcttgt cactagctaa ttccttgata ttgttgatat aactatctaa atcctttaat
    31141 atcattaaat ttccttggtt tgttagctcc tgtttccgtc tgtccatttc tttcctaaca
    31201 tgctctttat tcatccaatt atagattgtt tttctattta ctccaataat tctggcagtt
    31261 tctgttactg tagcacctgt aatcaataga gttaccatat tgcactgatc ttctgtcaac
    31321 tctttacctt tttcatagct actatcgatt atttcaactt cattcacatc caattaatca
    31381 cctctttact cagcttctaa ataaacattt aaatatctac atccctataa atcccttgaa
    31441 taaatgacct ttaatatata ttttcagaca ttttaataaa tctatcctat tctttggaaa
    31501 ttctattatt gaggctcttt acattctaat aatgtcctgt tgctgaaaaa cgtaacattt
    31561 aaattttaga taaaatacaa gatattttaa tttctttttc ttttctttaa cttattagta
    31621 aatttagtta ttgcttttac ttcttcatcc ttatctattc ctatatacct tctagttatt
    31681 tctattgtct tatgtcctag aatctttctt ataagttcta aagcataagt cttattctct
    31741 tcttcatcat aaatatgcct tgcaaatgtc tttcttaaag agtgtgttgc aacattttct
    31801 tttattccta ccttttctgc tgctcctttt attattcttc ctgcttgcct tgtttggata
    31861 ggcctattaa acttctttct cttaccactt ttaaataaga actcctctcc aggtttatct
    31921 tgagtatagt tttctatagc actctttatt acatcagata agaaaacttc tctatcatag
    31981 tctctttttt cttctataac tctaatccaa tccttagata ctcttatctt cttccctttt
    32041 ttatctaact ttacatctgt tacatctgca actttaagtg ctaatatatc ccctattcta
    32101 agcccagtac acattccaaa taaagctaat aagtaatttc tttcgttcca ttcctctaga
    32161 aaattcaaga gtctaaatac atcctctata tcttttattg gattaacttt ttgtccacta
    32221 tgtgcttcca ttcattaaaa acactcctta ttaccatgat aattttttct ttgcctatct
    32281 tgcaccaata tttttaagtc ttcaactaca ttagcaaaag gatctgtgat tatagaggtt
    32341 atatcatttg tactatattt tctttctctt gcataagtat tggtctttat tctaaatcct
    32401 aagtcattta cagtttcagt tacttttaca acattttcta attgcaaata taatttaaat
    32461 acaaccaact caacgtgatc agactcatca attatacttt catctaaaat ttttagatat
    32521 gagtctatct tattaatatt agaatcataa tttttcttaa ccttcttttt tagtttcaag
    32581 agtttttctt gctttttctt tataaaatct aaagaacatt ctcttccata catgctatga
    32641 gtttctctca tcacactgtc ataatacttt aaattatctg gtacatatgc agcacttgaa
    32701 atttcttctg ttatatcacg tttaatcttc aacacctctc tagacaataa aaaagactta
    32761 gaacgtaaat tccaagccat tttttattaa atatattttt ctcacaatac aatcttatca
    32821 tgattaaaag acacaaaaca gacataaatt ggtcacaaat cggacatttt ttagacacaa
    32881 aacagacata aatcggttta aaatcgtcac aaatcggaca tttttatagc aatcctttta
    32941 tcttatctat aatatcaaat ctcatttgct tacacttact cgctgaaaaa ccaatcattc
    33001 tgccaacata ttcccaagat gtcctgctac ttgaaaagta tttatactgt actatctttt
    33061 tttcttcttc acttaatatt ttcattgcat tatctatttt ttctattatc aactctttat
    33121 gtctcttctc tttttctttt aaggcaattt tcttttcttt ttcttcagtc tctatttcaa
    33181 ttacactttt aatatcatat gtcttgcttg tcttttcttt aatttcaact ccactacatc
    33241 ctctgtagtc gttttttatc atctgcatat caatgtctat tttttctatt tccgctttta
    33301 tttttttata attatataac ttaccttctg cactactaaa caaattgtct tttttgctag
    33361 attgcattac ctcacactcc taattatgtt ataatgatgt tgtgatgacg gttttagaat
    33421 tttgacaagc aggagcgtga agtaatgctc cttttttctt ttttgaaaca tatcttattc
    33481 tttaggcact aattgcaact tgtaatcatc agaactatat aatttataac ctcttacctt
    33541 caagtctaat tctagttgac ttaaactttt ataagcacca aatgaaccat atccattctg
    33601 catatttata ataacatacc cacctattgt ttcctttaaa gaaagaatca tatataactt
    33661 attaccacca ttaatttcaa caatatctcc tacttttaat tctctattat tttcagttac
    33721 ttctatcaac atattatatt atctccttac tttaattaat attttttaga aactctccca
    33781 ttttcatttc atcaatgttt tcattaattc ctttcatagc acttaagtta accaaaggca
    33841 cgcacaaatc ccatagctta tttacatcta aaaattgtat ttttgaatta aattcttttt
    33901 catcaacgca ttttaattta ttattcatat taattaattc attaatcatt gaatttttaa
    33961 ttaaattaag tataccatct cctatttctt tgagatccca tcctcttcca tttaatttct
    34021 tattatgtat ctctatacct tctgcaatat aatcttctaa ttctaaaatt tgaaacattg
    34081 ttaaaatagt ttggcaaaca tctaatcctt ctgcaattat atctttggtc attcctgtat
    34141 ctatagcctc tttaaactca gttacttctt catcaatttt tgccaactga tcctctacct
    34201 tccaattagg tatactgcta attaatttca aatccatctt taatatctcc cttcattcat
    34261 ttattgaagt cccaaccttt tttgagtatg cttattcatg aatattattt catatctggc
    34321 cttcttcact ttcaagccac ttttttcgac cctctaaaca tcctcctaag caatcttgaa
    34381 aatcataagc acaactagta catccacttc cgccatttgc aagaaattca gccatttcta
    34441 ttttgctcat attttttatc ttttcataat tagtcatctt cttattcaag ttacttccaa
    34501 ataatctgtc ataacatttg tcacatacat cttttccgac actcattata ttaccttcat
    34561 catcaattct gaataccttt atgtttagaa tttctgctat tgtttcacca caaaaatcac
    34621 actttataac acaactcatc tttaataccc ccttttactt tctttagaac tcatatcttg
    34681 atacatacat ccacaatcct tacattgcca cactattcca attaatgttt caatcttaat
    34741 atattaccat tacaaattgg acactcattt tccctaccac caattatttt agttttcatt
    34801 ttacctccaa taaacttaaa tcatttatta tttacctagt atattaattt cagtttttaa
    34861 ttttttaagt ctcctatata ttgacgtttg gctagttcca agcactttac ttatttcttg
    34921 ttgagtataa cctttttctc ttagacaaac tattttctgt atatctttta tctcacttct
    34981 ttttatacaa gatctaataa cacgaacatt ttctatatct acattttcat tgccagccaa
    35041 tctatcttct attcttatct cttctccata attatctcta taaatacact tttcaaaact
    35101 atctatatca tttctataat gtttcttaac atatccagtc gtaaatcttt tcaaatgtcc
    35161 tgatatatac gaaaatgcta ttgtagaaaa ttttccttta ctttcatcaa agcaacatgt
    35221 tgttttatat aatcctatat atccttcttg aaataaatct tcttttaaat atggatacct
    35281 tcttaaaaag gatttgaaat atctatttat aataaaatgt acaagattta tattatcttc
    35341 aaataattta tttttctctt ctaaacttct catataatca ccatccaatt taaaatggaa
    35401 tatcttcatc atctaaaact tgaaagccta tttcttcatc ctttggcttg tgatcaagcg
    35461 cattaacaat ttttgttctt acttttgtga aagttttttt ctctccatct ttttcatatt
    35521 tttctatcct tatttggcct tgaattgaaa ccattcttcc ttttgcgaga taattagcac
    35581 aaaattctgc tgatcctccc ataacttcaa ttggtataaa atcaacttct ctagttccat
    35641 ccttttttgc ataaccccta tcaactgcca atgcaaaagt agcaactgga gtaccatact
    35701 ctggtatata tcttaattct ggatctcttg ctaatcttcc aactaaaact acattattca
    35761 tattgcaata tctccttcat tgtatttatt ttctctatat tccatttgtt ttacaatctc
    35821 aactgcttca ttaaaggcac aacctctttc aatttcttta tttgcagaca taattgcatt
    35881 agcaattttg tctgtaaatt ctgctctttc taaaatgttc atatgagaaa tacttttcat
    35941 ttaactccct ccttgtttta ctaagaggac ttataataag tcctcttaga ttttcaatta
    36001 agcaattata tttacatttt taaaatcttt taattcttct tgcaagtacg ctttaatatt
    36061 taacattgct tgatttttcc atgctccacc atctgcttca tataatcctg ctgctggtcc
    36121 agtactcatt ctgaaaataa attcactttc tggttgctct acttcttgga aagtacgata
    36181 aggaattaat tttactctgt ttggaactat tgcttctgat acactagcga cacctgtttt
    36241 tattgttaca gattgagaaa ccccatcatc tccaacattt tttactgtag attcttggat
    36301 atttcctgca atttttaaaa gtgtatctct atcattattt tttataaatg ctgattgtag
    36361 cattatatta aaagtgtcta aatctaaagg tctgtcaaat cttttatctg gaagtagtgc
    36421 tacacattca atataagttt ctctatctct attttttctc aaagcagatt ttaaaagcac
    36481 ttttcttgga gttaagactt gtattaataa caactcatct tcttcatgat ctatgccact
    36541 ttttatataa tctactaaac cactcaatgt agttatttct aattcattag cttttggttc
    36601 ttttattatt tctaatgcat ctgttgtgta cttaaaccca tctacacata ctgtttctct
    36661 ttttcctaac ttaactaaat attccattgc atttcttatc attttttatt ctccttttta
    36721 tttaaattta tattttattt actttcttaa tctactttga acttgcattt ctaaaatcaa
    36781 ttacaccaga actattttct tctatttcat cagtttcatt actatctgaa acatctatag
    36841 acaactgacc agctagttga tttttaaatt cagatgcttc aactcttcct gtctctaaat
    36901 cttttcctat tataacttta gttgttgtcg cttttgcttc tactatagtt gctttggatt
    36961 ttatatctac ttcagcaaga tctctatctt catttgcttt aaacactatc tctaaactaa
    37021 tttttcttgt ttttttaaaa tctgtatttg gatctgcaat gttatctaaa actttttgta
    37081 actctgtatt aaacttttct tttagaactc cacctgcaaa tttttctaat gctatcatgt
    37141 aaaatcactc ctttatttat ttaaaataat atttttctac gaggaaatta attaataaac
    37201 tttagctcta atttaataac acttttaaaa ctttttataa atttatatag cctttttatc
    37261 cttacactac ttttaagttt ttttgctagc atatctaaac ctgtgtgtac attgtcagga
    37321 aattaattta tataaacctt gtaaatgcta tattttcaaa ctttttttag tattgaaatt
    37381 caatactttt tcaaaatccg tttttcaata ttgaaaaatg atttttttca atattgaatt
    37441 tttaaattat tcaatattgg tttttcttat aaaatcatta cttaataaaa tttcctattt
    37501 tttatacttt ttttaaaata catcttctgg aatttctact tgaacatatt gtttatttac
    37561 atcatatttg taatctctgt ttaactcctc actagtcgtg taaagtcttt ttcttctttc
    37621 tgaaaaattc attttaaaac ttatattctg ttttcctgtt attctatttt ttaaaattaa
    37681 taaataagtg tcgtctgtct cttcttcttc atcaacaact ctgtaagttg ataaaatata
    37741 atctgcataa ttaacaatct cacttgctcc agacacatca tacatagttg gcttcatgtc
    37801 acccatattt ttacgaggat gtgcaactaa aactattact aagttatatt ttttagcgag
    37861 attttttaag ttgcttacta tctgctcttg tctctcatat ttatctgcct tattgccagt
    37921 ttttattgtc attagattgt caagaacaaa gaacctaacc ccttttttta aatataaatg
    37981 ctctatggta tttagcagat ttacttcatc acttattgca tcttcatcat agagaaaaaa
    38041 tttatcttct atccagtctg atataagctc aacggcgtaa tctggaatat ctgtgtatgt
    38101 tcctccatag acacttttat attcttttat gtgataatca ttagctacag tttttctaaa
    38161 ccaatccata gacatgaaag agggtaattc tccagaatac aaaaatgctt tttctccatt
    38221 cgtaattgct tgtgctataa actggttaac tatagttgat ttaccagatg caggtttacc
    38281 agttaaaatt gtaagagttc catatctaaa tccatctagc atattgttta gtttattaaa
    38341 acccgtgtct atggacttgt gtacgccttt ttcacgtttt atttttgatg cgtcaatgta
    38401 attatcattt acactactgc tcgtcgtcac aagtgctttt aaatcgttta tatctataat
    38461 caattcatct atacttgtat ctgaatttga attattaatt aattcatttg ctaactctga
    38521 aatttgtctt ttaagtaact tctcttttat gatttttata tagtgatcta tgtttgaagt
    38581 agttataact atagttgtta aactggtaat gtaactaacg cctccaatat ctactaatct
    38641 atctattttt tctaattcat tttttaaaag caaaagatct agtggtttat ttgagtcaat
    38701 tattttcttc atgcaagata aaataatttt atgtgactca aagtagaaat cattttcttt
    38761 taattccctt attttgtaac ttatagagtc atttataaga aaacttccta gaatgttttg
    38821 ctctgcttct aaagagtaat tcatttaatc acccccagct tattattaaa atgtcacaac
    38881 taaaggaatc accctttcga cgtcatacaa taccccacaa atacgatctc ttggaacatt
    38941 tttgtaaact aaaatatcaa ttatgtcggc aataagtttt gcatagtatt ttggagtttc
    39001 aaatccataa cttctataag caagtttttc ttcaacaaat tgttcgcatt tacttcttgt
    39061 acttgttcta gtttcacaat ccttactttc aaatatcatt tctcttattt ttggatcagt
    39121 tcttaatcct tcaataagtc tttgtatatc ttgatctgta agttcatgtt tttttctttc
    39181 cacttagcat acctcctgtc tttaactatt tagttgcttc ttttgatttg aaactattct
    39241 acaccatgat aatttacatg ctataattaa tttaaacata cttccaatat gtttaaatta
    39301 attagaaagg tggtgagagt atgagccaat atcgagttat aagcaaagaa tgtcctgtaa
    39361 taaacgaaac catttctctt acttgcgaat attcagctcc tccaggtact tttggatcta
    39421 agttaatgtc aactagatgt gatggaagtc atatttgcga ctcttcttct aatgaagaat
    39481 gtcctattct aaaaaacttc ttagatagta gctttgggat tttcatctgg aaaacaatac
    39541 ttaaatgcag tcacttttac acctgttatt ttgcttaatt tagtaaaaga atgatacata
    39601 caagtaaaat cgccctcaca ttcttgcagg taatcgcaaa cttcacatga tgtgggggct
    39661 tctctattcc ctctaaacac catctccata atttcatttt gcaaatgtct tgctatgcaa
    39721 tgtaattctt tttcagtaag ttctaacttt tcctctttca tgagcaacct cctaatttat
    39781 ttaacattta attctttatc tgtttcattg agtttgtaac taaatttaat tgacctgtgt
    39841 tttaattcta cgccatgata atttgcatgt tataattaat ttgagcatat tggcagtatg
    39901 ttcaaattaa ttatagggtg gtgatgaata tgtgcaaaat taaaacctta agagaaaaat
    39961 gctctcttat tgacgataaa gttgaatttt ctggtgaata taaaactttt cctggattcg
    40021 ctggatcaaa acttttagag tgcgaatgta ataaaaatta catttgcaaa attcaaataa
    40081 tgactgctaa tagcaactgt cctcttttta aaaaagcacg tcaattacaa tactaaaatt
    40141 ttaatttact aaatttattt agtaaaaata tctacacctg tcatttcact taattttata
    40201 aaagtgtcta tatgggtgaa atagtccttg cattcttcga agtaatcgca aacttcacaa
    40261 gatgtagggg cttctctgtt tcctctaaat gctatctcta acacttcatt ttgcaaatgc
    40321 cttgcaatgc agtgcaaatc cttttcagta agttctacag ttttatctgc cataaacaat
    40381 cctcctaatt tatacttaac taaatatctg acttaatctc gaactcaata ttaaattggg
    40441 caagtttatt aatcgattct tggagttgac aaattcgctc attgacattt tctatcaatt
    40501 cctcaaattc cttaatatcc ctaacaccta agaataactt cacattagaa ctattcaaat
    40561 aaacagcctc ttctttttca ttcatcctat ctacctccta tactatttag tttttaaagt
    40621 tctttaaagc cactgagtgg attcttagcc tttgtaactg gttgcaatat acctttgcta
    40681 gtatcagcat tcccccaagt caacaaagct ctgtaatgac tcttatatct actcccttta
    40741 ccatttacga tatagttttc taaactaaca attttatcat gtatgtattt cttcccaaac
    40801 ttgcttataa gtttgtcata ttcatcttta gttagtttta ctttttctat atctaaatct
    40861 aaaaatgata ggtctaaata atcagtttta ggttcatttt gtggagtggt attacttttt
    40921 atataagtct ttttattata agtcttttta ctgtgcacct catgcacaac aggtagtgca
    40981 tgaggtgcac tagggttgtg catcatattc actacctgtt gtgcatcata ttcaccacct
    41041 aatttagctg ttaaaacaaa atatatgttg ctttcttttt catttgagtt actttttatt
    41101 ctattttctt ttttaattaa ccctttattt tctaaaattt taactacgct tttggcttgc
    41161 ctttcactac atccgcattt caaagcgatc gttttatagc ttggaaagca acaactttca
    41221 ttatcagaat atcttgcaag aactatataa atcatttttt catagattcc taaatcttct
    41281 ctatctacta gatcattctc tagccaaaac cattcccttt ttctagcatc tttcaagaga
    41341 tccatttcat cacctccttt ctaaaagata tttcttagtg taaaggtttt agattataat
    41401 cattatattt tatatcatct attgtcaata ttttagttgt aatgcattct aattcattgt
    41461 catagtactc caatactatt tcgtttttat ctatatgagt aataacgcct tttacttcta
    41521 aatttaatct tatagaggtc attttatatg ttttatttaa gctaaacatt tttgtaatca
    41581 tttcatcatt cactttaata cctcccaact tatgaatgtt ttatatccat aaaattattt
    41641 tctgacaatt catttgctat cttcatacct tccataattc caagaactcg atctctctgc
    41701 ttgtctttta atgaagaata caatattcca aattctgttg attcttttat gctctttgat
    41761 attttctttg tatctacctt tttcattatt attcacctct cttgtaatat gattttatta
    41821 taaatcattc atgtgaaagt ttcaataaaa atatgaaaat ttcacaatta ttttttattt
    41881 tttcattttt gttttgattt tttcacattt atattgtata attatactta agaggtgatt
    41941 aaaattggaa aatataaagg atgaaagtgt tttaaaaaca ttaagagagg cactggggtt
    42001 aaatcaaagc gaattagcaa atattttaaa aatgacacct tctgctatat ctaaaataga
    42061 aaaaaatggc aagcctttaa gtgatagaat ttcgtctcaa ttgagcaaag aatttggcgt
    42121 tagagaagaa tggcttaaga acaaagaagg caagatgttt gcagattcat cagatgatga
    42181 tttagttgca gaaatagcag caaatataat taattcagat gacaagttca tgaaaaatgt
    42241 cattatagct tttaacaagc ttacagaaga tcagagaaac tttttaatta attttatgaa
    42301 agagttagat aaataaagcc gttaaaataa cggctttatt tattgagaat tgtttttata
    42361 aatattaaga ttgattttaa tttttttaca tcatttgtat tttcaattga atttataatt
    42421 tcctttttta aatctcccat agaatccccc atatttttaa aatattctga tttatatttt
    42481 tacaattaca ttgcttctga aaaataacag acttccccct tttacaattt ttaaattttt
    42541 atcagctact aaattaatgt tttttgcttt tgtatgtcat gccttagatg agtttgaaaa
    42601 tttgattgta ttcaatattg aaaattaaga tttttgatta tttttaaaaa tattttacgt
    42661 acgtagtttt atatgcattt tatatgtaat ttcgatatat atcttcttta tgttacaatt
    42721 gtaacacctc atttaacatt aatcaaatgt aaaattttac caatttaaat tattttttaa
    42781 aatttaattt tacattaata gaatttattt atataataaa ttaaaaaagg actatggact
    42841 atagaatttt acttctatag tccatagtct tcctataatt taggattgat ataaatcttt
    42901 tagaaaccag ttatttctga taactcataa gatgcaattt ctccatctgt attcattccc
    42961 attgctttga acttccaagt accatctttt ttaagatcgt ttatattatc cattgcagta
    43021 cctagttgat ttccatcctt atcatataaa gtaaatgtta cttgtgcata tgatatatca
    43081 tgaccactgt tgtttttaat aacacctttt atatacgttg ccatactatc agtttctgat
    43141 gttatatctc caactatttc atatttttcc tttttaggct cttctggttt atctacacta
    43201 gctgttttac ttgttccatc atcattactt tcagcagtat catcagtacc acttccacta
    43261 gtaccaatag cgtataataa aactaaaact actacaacta taaaccaacc tcttttataa
    43321 aatggttttt tgtttttagc cccacagtta ggacatgatt tagcatttga tgctatttcc
    43381 tttgaacatg atttacattg aatcattttt gacattttaa aatccccctc taattaattt
    43441 atgatgacct caatcatatt atagtataca actaatgtct aaaactgtaa tatcatgaca
    43501 tcaaaagaat tttattaggt atatagcaaa tattttgttg tactaaatgg cattcaataa
    43561 agactttcac ttatattgta tacatttttt cgaactgctt ttaagttata atattaaata
    43621 aataatttat ttaatattat aatacgtctt tataattagc atattaaagt attttttata
    43681 atcagaatta attggaaaaa attggaatgc ataattagac atttttcatt gtaaattaaa
    43741 ttctattgta atagtattat agtatatcaa aaaacggggg tgtagtactt gaaaataaaa
    43801 aagattccag agtcagaact tttggtaatg atgtacattt gggatagtga agatgaagaa
    43861 gttgcatcta ctgaaatttt gaaaacatta ggtgaaaaat atgaatggaa aaaatcaact
    43921 atgttaactt ttttaagaag gttagttggt agaggatttt tagaagttgt taagaaagat
    43981 agatttacat attataaagc attaatagaa aaagaagaat atttaaaagt tgaaactaaa
    44041 agtttcttta gtttctttca cagaaattcc tttgaaagtt ttataagtgc attacatgat
    44101 gatgaagaaa tatctgcaaa taaattaaaa agtttagaaa agtttattga ggattgggaa
    44161 gaagataaat agagagccaa tataatttta tttggttctc tatttttata tttgaccttc
    44221 tatttccatt tttaagcaaa tttgtatctt tttaggtaaa taatatcaag tttgcaatta
    44281 aaacgtgtca gacaagcctt taaatgcttt taagctatat ttaataaact ttatttttat
    44341 caatttcctt tttccactca tcaaaattac aaatccgata aacttttctt atttttggaa
    44401 cttctctaag tttttctttg tcatattcat atacatttac tttttttgtt tcaatgtatt
    44461 tattttcttc taaagtttta acagaagaag ttatgatatc taaattattt ttacttttag
    44521 aacttagtcc tatattttct gctataaaac tattagttgc aggtttaaaa ttagtttctg
    44581 tagtgattgt tttaagtaaa aaatatactt ttattgtatt tgatttaaat ttattgacca
    44641 tttttttaag catttcataa tttatcaatg caaatttgtt taaatcaata ttattttctg
    44701 acggtaaaca atatacaatt ccatttaggg tattttctat ttttagaatt ttaaaatcta
    44761 acttctctag tttttttata tttctttgga ttgttgcttt atttacattc agttcatttg
    44821 ctatttcttg aatatcaagt ttattcttat acaaaattct atttttatgg gtattaaaaa
    44881 tgtcactatt ttcagcactc tttattaata gtgctccata ttcttttata taatattgct
    44941 tatcttttaa acaatatgtt gctattggta agtaaatttc tttctgaatt tgttttctgt
    45001 tttcaagcat aaaaaattct tcatctccaa tcttttataa gaaattattt ttagtaagtg
    45061 tttaaagtat actagaaaaa taaatactaa agttttttaa tataaaaaaa gaatagttca
    45121 gaacatcttt tctagtcttt aatagttttt aaaacagttt ttaaagtctt taatagtttt
    45181 tagacaagct ataataattg gtatatctag gtttcagaga t
//
LOCUS       DAGQWR010000010        44945 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.010, whole genome shotgun sequence.
ACCESSION   DAGQWR010000010 DAGQWR010000000
VERSION     DAGQWR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44945
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.010"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(199..768)
                     /gene="yfcE"
                     /locus_tag="MIW95_000462"
     CDS             complement(199..768)
                     /gene="yfcE"
                     /locus_tag="MIW95_000462"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBY3149818.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(793..1593)
                     /locus_tag="MIW95_000463"
     CDS             complement(793..1593)
                     /locus_tag="MIW95_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBY3149819.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            1728..2600
                     /locus_tag="MIW95_000464"
     CDS             1728..2600
                     /locus_tag="MIW95_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3149820.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDREKFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVRGCSI"
     gene            2588..3274
                     /locus_tag="MIW95_000465"
     CDS             2588..3274
                     /locus_tag="MIW95_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBY3149821.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            3326..3868
                     /locus_tag="MIW95_000466"
     CDS             3326..3868
                     /locus_tag="MIW95_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149822.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITESTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            3947..4414
                     /locus_tag="MIW95_000467"
     CDS             3947..4414
                     /locus_tag="MIW95_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBY3149823.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNNKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKSEKWVDVCKNW"
     gene            5122..6258
                     /locus_tag="MIW95_000468"
     CDS             5122..6258
                     /locus_tag="MIW95_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBY3149824.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLKPLASGDKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            6317..7099
                     /locus_tag="MIW95_000469"
     CDS             6317..7099
                     /locus_tag="MIW95_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBY3149825.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            7118..8128
                     /locus_tag="MIW95_000470"
     CDS             7118..8128
                     /locus_tag="MIW95_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBY3149826.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            8147..8944
                     /locus_tag="MIW95_000471"
     CDS             8147..8944
                     /locus_tag="MIW95_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBY3149827.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            9042..9887
                     /locus_tag="MIW95_000472"
     CDS             9042..9887
                     /locus_tag="MIW95_000472"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBY3149828.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            9974..11149
                     /locus_tag="MIW95_000473"
     CDS             9974..11149
                     /locus_tag="MIW95_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBY3149829.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            11344..12246
                     /locus_tag="MIW95_000474"
     CDS             11344..12246
                     /locus_tag="MIW95_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBY3149830.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKKEK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            12260..13264
                     /locus_tag="MIW95_000475"
     CDS             12260..13264
                     /locus_tag="MIW95_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBY3149831.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(13408..13635)
                     /gene="acpP"
                     /locus_tag="MIW95_000476"
     CDS             complement(13408..13635)
                     /gene="acpP"
                     /locus_tag="MIW95_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBY3149832.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            13807..14232
                     /locus_tag="MIW95_000477"
     CDS             13807..14232
                     /locus_tag="MIW95_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149833.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            14280..14486
                     /locus_tag="MIW95_000478"
     CDS             14280..14486
                     /locus_tag="MIW95_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149834.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            14668..14847
                     /locus_tag="MIW95_000479"
     CDS             14668..14847
                     /locus_tag="MIW95_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149835.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            14881..15081
                     /locus_tag="MIW95_000480"
     CDS             14881..15081
                     /locus_tag="MIW95_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149836.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            15334..16350
                     /gene="ccpA"
                     /locus_tag="MIW95_000481"
     CDS             15334..16350
                     /gene="ccpA"
                     /locus_tag="MIW95_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBY3149837.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(16473..16871)
                     /locus_tag="MIW95_000482"
     CDS             complement(16473..16871)
                     /locus_tag="MIW95_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149838.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGGQQKKSKGLFKKTKKSNPHE"
     gene            17083..17988
                     /locus_tag="MIW95_000483"
     CDS             17083..17988
                     /locus_tag="MIW95_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149839.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            18103..18283
                     /gene="ssrS"
                     /locus_tag="MIW95_000484"
     ncRNA           18103..18283
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="MIW95_000484"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            18466..19683
                     /gene="cdeC"
                     /locus_tag="MIW95_000485"
     CDS             18466..19683
                     /gene="cdeC"
                     /locus_tag="MIW95_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBY3149840.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDECNPCNPCNPCKPNPCNPCKPNPCDDKCECHDKCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSIPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(19784..21106)
                     /locus_tag="MIW95_000486"
     CDS             complement(19784..21106)
                     /locus_tag="MIW95_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBY3149841.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(21157..21951)
                     /locus_tag="MIW95_000487"
     CDS             complement(21157..21951)
                     /locus_tag="MIW95_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBY3149842.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQYEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            22061..23506
                     /locus_tag="MIW95_000488"
     CDS             22061..23506
                     /locus_tag="MIW95_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY3149843.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSSNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTNKYKLSKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISVISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            23535..23744
                     /locus_tag="MIW95_000489"
     CDS             23535..23744
                     /locus_tag="MIW95_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBY3149844.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            23764..25158
                     /locus_tag="MIW95_000490"
     CDS             23764..25158
                     /locus_tag="MIW95_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBY3149845.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTALPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGFDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            25398..25583
                     /locus_tag="MIW95_000491"
                     /pseudo
     CDS             25398..25583
                     /locus_tag="MIW95_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(25692..26858)
                     /locus_tag="MIW95_000492"
     CDS             complement(25692..26858)
                     /locus_tag="MIW95_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBY3149846.1"
                     /translation="MKGGVRKRGKKWYYYFDAGIVDGKRKKVERVGGNTKKEAEKSLR
                     DAINEYENAGIVFDETNMSLSDYLNFWYKEYVLLNCKYNTQESYRNLIENHIEPRLGK
                     CKLKSINPAIIQEFLNNKSKETYTQNGEEKHYTKGVLKAIYVVLNAALKSAVYPYKLI
                     KENPVQYASIPKNVLKVKNESDNKTITLDEFNKILEIYPKNTNIYIPLLIGFHTGMRK
                     GEILGLCWDNVDLDNNIIKVRKNLIKRKVSEFELASPKTKTSIRDIKIGDTLSRILKE
                     EKLNQKKQKIKIGKWYKETEYDWVCRKKDGSFVNHNNIDAAIRTINKKLNINFNFHCL
                     RHTHATLLLENGANVKYIQQRLGHSQLSTTMDTYSHVTSKMESETIDILEGILQ"
     gene            complement(26996..27202)
                     /locus_tag="MIW95_000493"
     CDS             complement(26996..27202)
                     /locus_tag="MIW95_000493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149847.1"
                     /translation="MNLSFNKKKKFELSKEELELIENWLGNKYAGDMTIPAIVDFSLE
                     TDIEYEKIVVYLAEKVLESRDKKH"
     gene            complement(27261..27482)
                     /locus_tag="MIW95_000494"
     CDS             complement(27261..27482)
                     /locus_tag="MIW95_000494"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3149848.1"
                     /translation="MLKELRKKKKLTQIELAKRVGCHRSQISRLENNENKDLTIPALI
                     ELEIALGLEEKYLVNYFADEYIKKRKLHK"
     gene            27766..27903
                     /locus_tag="MIW95_000495"
     CDS             27766..27903
                     /locus_tag="MIW95_000495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149849.1"
                     /translation="MNKTKYYDILKLNLLMKKLKELDKNKINEYKIKVKEIHKINKKE
                     E"
     gene            complement(27940..28335)
                     /locus_tag="MIW95_000496"
     CDS             complement(27940..28335)
                     /locus_tag="MIW95_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3149850.1"
                     /translation="MNRIKELREEKGISLDKLSEDLHINKSTLSRIENGLREPKKSTI
                     EEYANYFDVSTDYLLGRTDVRNSLFINKNEKDYDAENFKTEKELIENMYLDEDMKEVF
                     NIFSELSPDAREKALKVAELFLLDEKNKK"
     gene            28514..28747
                     /locus_tag="MIW95_000497"
     CDS             28514..28747
                     /locus_tag="MIW95_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HBY3149851.1"
                     /translation="MYLNRLEGLMKENRHTQKNVADILGLSSYGFRLKLKGKNEFKAS
                     EIKKISKLYNVSADYFFSDEVAKIAIKEERGNK"
     gene            28747..29535
                     /locus_tag="MIW95_000498"
     CDS             28747..29535
                     /locus_tag="MIW95_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBY3149852.1"
                     /translation="MKNLTIIKQNNQFLVESREVAELIEKKHDNLLRDIRGYKKILED
                     SSNLKSQDFFIESTYINTQNKIQPCYLLTKKGCDMVANKMTGEKGIIFTAIYVTKFEE
                     MERELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQEKDKVIQLQQPKVLFADSVA
                     SSDNSILIGELAKLLRQNGIDTGQNRLFDWLRNNGYLIKRKGEDYNTPTQKSVDLGVI
                     ETKEGTRVHPDGHTSITKTPKITGKGQIYFINKFKKNNQISMLG"
     gene            29592..29729
                     /locus_tag="MIW95_000499"
     CDS             29592..29729
                     /locus_tag="MIW95_000499"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149853.1"
                     /translation="MSNKKKYTLSITEELYNKCKEKANQKGMSVNEYILLVISKNLKN
                     I"
     gene            complement(29741..29908)
                     /locus_tag="MIW95_000500"
     CDS             complement(29741..29908)
                     /locus_tag="MIW95_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBY3149854.1"
                     /translation="MPSKLPRYTLRIDNNLLEKIKYIAESEGRSANKEIEQLIKKHVE
                     SYEKINGKINV"
     gene            30061..30207
                     /locus_tag="MIW95_000501"
     CDS             30061..30207
                     /locus_tag="MIW95_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149855.1"
                     /translation="MPDELNEELHRQSQRKGLSKNAFIINILWKEFEDLQDLKDEQEV
                     DKYE"
     gene            30200..30838
                     /locus_tag="MIW95_000502"
     CDS             30200..30838
                     /locus_tag="MIW95_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149856.1"
                     /translation="MNNLQIFEKMEFGQIRMAEIDNKPYFVGKDIAKSLGYKNTNDAI
                     LRHCKGVVKHEGFKINGIKIALITEGDVYRLIVGSNLPNAEKFESWVFDEVLPTIRQT
                     GQYQAQQNVITELTGTIGDLKGTIKEYKKLCKITCSKKQQYSKYIKNRLDIDKANKEY
                     NQVKARLFLILGVEKWEDIDFDTSNNLIQIIDDCIKVIKSERPYKQLSYFEN"
     gene            30897..31277
                     /locus_tag="MIW95_000503"
     CDS             30897..31277
                     /locus_tag="MIW95_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149857.1"
                     /translation="MKNNTSDSRVKYFCKCPYCGFDNEVEVKKGLKPKICCICTKEVE
                     YEKLEQQSALENTEIKGVCNQMKIKKLALATGEVFENIELKEFENKGECLPSNMVKVI
                     DGKQELLINKEFILSLEVKRKTTF"
     gene            complement(31267..31419)
                     /locus_tag="MIW95_000504"
     CDS             complement(31267..31419)
                     /locus_tag="MIW95_000504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149858.1"
                     /translation="MVIPIAKNTKQTSKTVASKASKILKDGRYSAASKSVAGSALSQT
                     KKGPKK"
     gene            31478..31675
                     /locus_tag="MIW95_000505"
     CDS             31478..31675
                     /locus_tag="MIW95_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3149859.1"
                     /translation="MAINDNINKILKDRDLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKIADYLDITLDELVGR"
     gene            31714..32148
                     /locus_tag="MIW95_000506"
     CDS             31714..32148
                     /locus_tag="MIW95_000506"
                     /inference="COORDINATES: protein motif:HMM:NF024139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3149860.1"
                     /translation="MGNISNFNLDKQEDKSFNDLDNISICFSEGIRKVVEMNLNNYKN
                     KISKYLNETSKIELLEPKELTIVISKGYPDYLMSVEEASKRLKIDKVFGYELIKNGLL
                     KSVDIGATKVSSYELDDFITRNQGKNIKEMLREMKELREGVI"
     gene            32276..32464
                     /locus_tag="MIW95_000507"
     CDS             32276..32464
                     /locus_tag="MIW95_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149861.1"
                     /translation="MKIIYKNKVYKVEQDKKLFRITYYDEQRGSKKFNKEKKIKRSVL
                     TRDIELVNLYLPAHLKIK"
     gene            32496..32639
                     /locus_tag="MIW95_000508"
     CDS             32496..32639
                     /locus_tag="MIW95_000508"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149862.1"
                     /translation="MEIEQATIRLPRELKDKLLKQAKVKGYTLKDMIVFILKDYLQNI
                     SQE"
     gene            32912..33070
                     /locus_tag="MIW95_000509"
     CDS             32912..33070
                     /locus_tag="MIW95_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBY3149863.1"
                     /translation="MTNERVRFTFRLPAPLLEKIKNRASIEGSSMNSLILHILWDYIK
                     EIENKEVK"
     gene            33070..33297
                     /locus_tag="MIW95_000510"
     CDS             33070..33297
                     /locus_tag="MIW95_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149864.1"
                     /translation="MVELVKEFDLQTIKVGNAVKVNCKRFGFEIDCIVVVATEKELNL
                     AYFDEGRGCMEYQALITEDIQDGDYEIKILS"
     gene            33395..33709
                     /locus_tag="MIW95_000511"
     CDS             33395..33709
                     /locus_tag="MIW95_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149865.1"
                     /translation="MESLKRIKKMVQKQLVLAELEINKNSKLYEELENKDRGLIDDIH
                     MREYLREKVAWERVKYAIENILGGINLEIKSKEHEESEDYKIFQLILEELERDKPIDV
                     QI"
     gene            33793..34302
                     /locus_tag="MIW95_000512"
     CDS             33793..34302
                     /locus_tag="MIW95_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBY3149866.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNETEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKRVKKNTLDRLKSNIKDCMELLGTKKVETVL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNIIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            34312..34920
                     /locus_tag="MIW95_000513"
     CDS             34312..34920
                     /locus_tag="MIW95_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBY3149867.1"
                     /translation="METNNVYIKLVNIQSTLKAPKSQFNSFGKYNYRSCEDILEGLKP
                     ILKEEKALVILDDNIVQIGNRFYVEATATLIDAETGEKVSTKALAREDETKKGMDLAQ
                     VTGSVSSYARKYALNGLFCIDDTKDSDATNKHGNEQKKKEVNESELNTLYSLGESIEK
                     DKNRVDSEVYKKFGKLAVDLTKQEYEKVLNGYKSILEKQKQE"
     gene            34930..35811
                     /locus_tag="MIW95_000514"
     CDS             34930..35811
                     /locus_tag="MIW95_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBY3149868.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDVKLSWQAKGLHSYLISKPDH
                     WKIYVNDLCKRSKNGRDATANILKELIENGYITRKPCRDSNTNKMLGGYDYEVYEIPL
                     ENPQKLKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQSNKDKTT
                     YIKKYFEKYIGVITPNNFIELMSYLDDGMEADVIIRAIDEAIANGVKNYKYVKTILNN
                     WIEAGVKTNLELTEYQNEFERKKKNKQDKKQSNSKAVNTPNVSKNKFHNFNETFTQYS
                     PDELDDIIKKSQKVKFK"
     gene            35872..36045
                     /locus_tag="MIW95_000515"
     CDS             35872..36045
                     /locus_tag="MIW95_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBY3149869.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKVAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            36061..36468
                     /locus_tag="MIW95_000516"
     CDS             36061..36468
                     /locus_tag="MIW95_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBY3149870.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKAKPVYAVGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKNDNNAKQEYVPPGLDPQGFQAIDDDDIPF"
     gene            36542..36790
                     /locus_tag="MIW95_000517"
                     /pseudo
     CDS             36542..36790
                     /locus_tag="MIW95_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861747.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36788..37039
                     /locus_tag="MIW95_000518"
     CDS             36788..37039
                     /locus_tag="MIW95_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149871.1"
                     /translation="MIIIRSQDRLDLMRVNRVEIYDKQVFVIFEDDVKRIGVYESNER
                     AIEVLNRIQEAIIAGTKFDIINKDGVRCNKEKVFEMPVE"
     gene            37053..37418
                     /locus_tag="MIW95_000519"
     CDS             37053..37418
                     /locus_tag="MIW95_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149872.1"
                     /translation="MLKVEKYFNGSVADNIFEDDLTLRNYLALYCCVYGVRKNGELVF
                     PTSEKMLIEFNVDKNRKKKKKGSKVKLINAKTGEEKIFDSIDSAACFLRLQSQAVYQT
                     IKKKTKTRSGWKAEYIKEE"
     gene            37536..37985
                     /locus_tag="MIW95_000520"
     CDS             37536..37985
                     /locus_tag="MIW95_000520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149873.1"
                     /translation="MSLIKYRGYDFENEKWIYSATIMWSNILECLVMLTEGCKWQKVS
                     NVGVCSGEWARNNQEICEGDILKGYDNSSDTSQYGVVKRDFNSIKLYLEWHYLKKFEG
                     EWIELINKTEIYHSRDYKVIGNEYENLEEVRTEFLERKESLENEYLS"
     gene            38088..38264
                     /locus_tag="MIW95_000521"
     CDS             38088..38264
                     /locus_tag="MIW95_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149874.1"
                     /translation="MDDRLEMINASVNYIQMICESSNIAIIAERGRVRILDLETKEKY
                     DLLKNKLEEMLEEI"
     gene            38374..39447
                     /locus_tag="MIW95_000522"
     CDS             38374..39447
                     /locus_tag="MIW95_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBY3149875.1"
                     /translation="MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHK
                     PKEDEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
                     RERIFIVGHFRGRSTRKVFPIERKSGKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGG
                     GGAKTGLYFIDLNKNSKVTINARCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQ
                     NGRRIKESGETMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDS
                     QLYKQAGNAVTANVVYEIAKRMG"
     gene            39527..39736
                     /locus_tag="MIW95_000523"
     CDS             39527..39736
                     /locus_tag="MIW95_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149876.1"
                     /translation="MYEYILRWQIGLSLENRKIHYTYGSKEALRKKAKALAKDENIVL
                     ITIDKVDEVIKNTISEKIIERFENL"
     gene            39748..40752
                     /locus_tag="MIW95_000524"
     CDS             39748..40752
                     /locus_tag="MIW95_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBY3149877.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVNQDMVLFFQKKISKVSR
                     DNDIRTAVFNNYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYSQKDEKKVAILKLDYNNSYTHSIEFKDDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNDKYHLRVLDKDAEKEEANSKFVTEFLNATKIKDDKYKTKKFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLREKHEIDINDFVDKTIKDDKLKDSFKEHMEEKGLVEGFSI
                     DKKWVDKKLKKRNIKTDNGFEIKGNLTDFEDPMKYTVRQNQNGSIDIVIKNVTFYEEK
                     "
     gene            40834..41163
                     /locus_tag="MIW95_000525"
     CDS             40834..41163
                     /locus_tag="MIW95_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149878.1"
                     /translation="MKIFLLTILLIIICILANYVKNRIYKKSINNLKYKYSVGEKIIY
                     HQINCYYNRMVGCEILEKCYSTKFRKRNTPLYKVKAYVGDNDTTWVIPEWRIECLATT
                     YGEFPKY"
     gene            41200..41571
                     /locus_tag="MIW95_000526"
     CDS             41200..41571
                     /locus_tag="MIW95_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149879.1"
                     /translation="MFNIYKVKIKTKRTLEQVRNQSVDFEYSEKGLKNTLKYYNLIDD
                     LKVIVVKFGDEYCLANYNEEDRKIIMEAHYLLEQDEYTGCYINEYERFKKDWENGNCD
                     GEACMVFSDDEIEIIEKLREG"
     gene            41574..41756
                     /locus_tag="MIW95_000527"
     CDS             41574..41756
                     /locus_tag="MIW95_000527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149880.1"
                     /translation="MNKRIKMKKRLIHKKCDERCVNYDFVISNNLITCNVCIGCKYKE
                     NMDKVCEENYKKLRSK"
     gene            41836..42177
                     /locus_tag="MIW95_000528"
     CDS             41836..42177
                     /locus_tag="MIW95_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HBY3149881.1"
                     /translation="MANIYCENYNCKNYFEDMCMLERIEINNFKECESYLEGKNELYE
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEVLEEELTK
                     INSHLELEDLR"
     gene            42303..42710
                     /locus_tag="MIW95_000529"
     CDS             42303..42710
                     /locus_tag="MIW95_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBY3149882.1"
                     /translation="MKVFLVIDGEPVGKERPRMNTYAKRTYTPNKTKNYEDLIKWLYQ
                     SKVKYYFEGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVIKVVADSLN
                     EIAYKDDTQIVEVVASKYYSDNPRVEVILEDVI"
     gene            42812..43660
                     /locus_tag="MIW95_000530"
     CDS             42812..43660
                     /locus_tag="MIW95_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HBY3149883.1"
                     /translation="MNENINKEITVLGTLEIEGMKFHNIEGGFGEHKKAMLVKDIAEI
                     HNRESRQINELINKNRKRFKDGKDILDLLGVGLDDTKIKELGFTQQSINSYRGLKNKG
                     LLSGIYILSERGYAKLLKILEDDIAWELYEKLVDGYFSMRKELNNPLLSASKELQAIF
                     MLDKKQEVLETKIENVNEKLENFMDDAPLFNIECESIVKEVKKVATKSLGGHGSKAYK
                     NKSLRGKVYNDIYHQIKREFGVDSYKAIKRCQLNKVLEIVNNYKLPIVFEEEIRLLNS
                     QLSIVS"
     gene            43763..44251
                     /locus_tag="MIW95_000531"
     CDS             43763..44251
                     /locus_tag="MIW95_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149884.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAVGYDEKVSPTYAFNSTVENEIIKKERDITRLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKVMDMLYPRIKENELPLF
                     KN"
BASE COUNT        17980 a         4860 c         7940 g        14165 t
ORIGIN      
        1 tataatatat aatagaaaca agtaaactaa gttatatttc aatatagcat ataataaaac
       61 aagtaaacca gattctattt aattagaagt ttaaggtatt gtacttaaac ttctaattga
      121 gatagaaata aaatggttta cttgttttta ttatgagttt aataaattaa agcttatttt
      181 atttttatta aaaatttact aatcattgat atttattata tttccagtat tgatgtcaat
      241 taagtttata tcaagttcaa gctcatcctc atcatcagtt tttatgatat ctataattgc
      301 gacagaatgg cttccatctt ttgggataga agtacttcct ggatttatta ctattaaatt
      361 atcatccatg tatagttttt ttacatgagt atgtcctaaa actaatatgt caccacccat
      421 tttctttgct ttgtcaataa tttcttcttc tgattctata taaccatgat tcaatataat
      481 tctaatttca ccaaattggc tcatgacata aggactctga ataggatgtt ctataaccat
      541 ctggtcaaca tctgcatcac agttcccttt tacaatcaca atgttttcta atttatttaa
      601 tgtctcaata aattttttag gattatatcc ttctggaata tcatttctag gtccatgata
      661 taagacatct ccaccatgta ataaaacatc acaatcagat aatacattta acgctttttc
      721 aaagtaaagt aagctaccat gagtatcact cattattcct attttcatat taaaaatcct
      781 cctaagtaca gtctataaaa atttttcaga tactctatgc caaaactcat agtttgacat
      841 tctaagtaat tttatttttg catcagatat actaactttt atattttcga tttgtctaaa
      901 tctgtgctca actccatcaa cgacaatcaa tacagaatct tcaaatctat attctggtgc
      961 aatctttata acagattcat ttgaacatat tatacttgat gtaaagcacc tatatgcatt
     1021 ggtatttatc ggatgtaaag gtgtaatctg cataagttca agatttatat caacaatact
     1081 acctccagca gaatagttat aggctgttga acctgtggaa gtagaaataa tcataccatc
     1141 tccactaaaa ttttgtatat gtttattatc aaggcttaaa tttaggtgta tagtcctaga
     1201 cttgtctcct cttataacaa cctcatttac agcaagcata ttagaaccac tagtagttgt
     1261 atatacttcc gcattaagaa gtgacatttc ttgaataata taatcttttt ttgtataagc
     1321 ttcaataaag ctatcaatct tatctggtaa aatatcagga aaaaacccca gatgacctgt
     1381 atttattccc attatgggaa tctcaggaaa atcgaaatct ctaactgttc ttaaaaaaga
     1441 gccatcacca ccaattgata taataagctc tgtgtctgga taaatatctg aatacacttc
     1501 aaagccagca ttgataagtt ttctagttaa gagttcttta gtctcaaggg acttatttaa
     1561 ttggtttgta tttatagtta taattctttt catatattca cctcttcata ctttgtatta
     1621 taacatagtt ttataattct taatttataa atttataaat gacctttgta gtgaatgtgt
     1681 taaaatatgt ataggtgggt ataaaaaaat gcataagagg tgaagatgtg tttagcataa
     1741 ggaatatcac aaaaaaatta ggaaaattca agttaaataa tataaactta gagttaaagg
     1801 aaggggatat tgttgggatt ataggaccaa atggttcggg aaaaacgact ttaataaaaa
     1861 ttataatggg gattattgat gcagatgaag gtgaaattga actttgtaat gaaactattg
     1921 aaaattctcc aataagcttt aaaaacaata ttggttttgt atatgattct ttacaatttt
     1981 atccacattt aaaggttaag gaatttagaa aaatagtttc tttattttat aagaattttg
     2041 atagagaaaa atttgatgaa taccttaata agtttgacat agaagaaaat atgcatattg
     2101 aaaatttatc taaaggtcaa tctgagaagc ttatgttatc aagcgcactt agtcataatg
     2161 ctaagttatt gattttggat gaaccaactg ctggtataga cccgattgta agaactgaaa
     2221 taatgcagta cttacaagat tttgtgaaaa atggaagtag tagtgtaata atttctactc
     2281 ataatactga taatttaatt aaaattgctg attatcttgt ttttataaat agaggaaatc
     2341 agatttttac tgttaagaaa gaattaatag aacaagaata taagataatt agggcaaata
     2401 aagcagagtt agaagcaatt aaagaaagta tagttggtgt taaagagtat aaatattata
     2461 atgaggcact tgttaaagtt ggagatagct tgaaagtaaa aagcctattg atagaaattg
     2521 ataagcataa ggtcaaaaat cctactatag aagaactaat gtattattat gtaaatgagg
     2581 tgagaggatg ttcaatttaa taagaaaaga tttaataata ggaattagtt cagatggtat
     2641 ccgtaattta aagtacatat tgctattttt tgtgttttat tttttcctta attcaatttc
     2701 atattataca gtaagtattg ttgttagtta tttgatattt ataaatacat ttgagtgtga
     2761 ttatgaaaat gattcaagga tttttataag gagtatgcca gttagtatag aagatatagt
     2821 ttactcaaaa tatttattag gtgtaggact aataatttct gtaacaataa ttgtgtcctt
     2881 actaagtaaa ttgacatcac tagtgttctt tagaaatatg gttttaaatg atgtattttt
     2941 ttctgtaaat atatttttag caattttgac gattttactg cctttaattt ttaagtttgg
     3001 atatggaaaa atgaaagttt gtggttttat agtaagtatt ttaatttact ttgtatatgg
     3061 aagtcttttg aagatgatta gtatgattgt atatcaagta aagcatttta attatagtaa
     3121 agttggagga gtatatcttt caaattatgt aactgatata gctaatacta agtatataaa
     3181 tttatatagt atgacatttt taacaataat aatttttata atatcaatgt atttttctat
     3241 taagatttca aaaaagaata agtttaatta ttaaaatttt aattaatatt atagcacaat
     3301 agcacaaaat ataaggagac taaaaatgaa gaagtttata aaagtttcat tttttatttt
     3361 ggggattttt acactattca tagctataaa taaattagta tatagagata tggtaagtgt
     3421 caaagattat gaaataaatc atttagagga aattgttttt gattttgata gcaggagaag
     3481 tagagatatc ggtactattg taagatttaa aataagcaat aatagtaaat atagttataa
     3541 attaaacagt gctaaaatga agtttgaaaa ttgtgtggaa aataaaaagg gaatgaataa
     3601 gtgttctgca tatgtaaatg tagatttatt taatgaaaaa aattctataa ctgaaagtac
     3661 tctgaaatat ggaataaaac caaataatca aggttatgtt gattttgtaa ttccaaaagg
     3721 actaaatttt gattataggt actttaatga atctggtatg agtgtagaat ataaaggaga
     3781 atatattgtc aatattccaa tggcaaaagg actttatcta gtagttggaa aaaataatga
     3841 tacatggaat gctaatatta tggaataatt tgaaaaataa atcaattata gatataatta
     3901 agaataatat gaaatttata aaaattcaga ctaggagggg tatttaatgt caataagtaa
     3961 tgtaagagag tattttaaac aatttggcaa ggaagacagt atattagaat ttgaacaatc
     4021 tagtgctact gtggaacttg cagctgaagc agctggggtt attccagcaa gaatagctaa
     4081 gacactctca tttaaaatag gagatgatgc aattttaatt gtgacagctg gtgatgctaa
     4141 gattgataat aataagtata aggctgaatt taattgtaag gctaaaatgt tgacaccaga
     4201 agaagtcttg gagtttactg gacatgctat tggtggagtc tgtccatttg gtttaaaaaa
     4261 ttctataaaa gtctatttag atgattcaat gaagagattt gatacagtat ttcctgcatg
     4321 tggcagtagt aactctgcaa tagaacttac atgtgaagaa atggagaagt tttctaagag
     4381 tgaaaaatgg gtagatgttt gtaagaattg gtaaaaaaat atagtaaact atagtaggaa
     4441 ttagtaaaaa actacctctg tatacaatct atacagaggt agttttttgt gtaaatattt
     4501 ataaatctaa gaggaaaatc taatttaaac taataatcca aaataaagtt aaaaaattct
     4561 gatttttttt cgaaacaata aaaaaatgac aaaaaagtgg aaaaaatgta ataaatattg
     4621 ttaaaaaaat aattagaaat tattgaaaaa ttatcctttg agtgttacta ttatagatgt
     4681 aagatattta aaaataaaaa atagtcagat aaaaatacga ttatttagct ctattaaaat
     4741 aatttttata cgataaaaat cttctgtaca tggctaaaaa acagatgtac agacaatatt
     4801 taatgtttaa tataccctaa tataaatatc gtaaacaaat tattttatct gtgaccagtg
     4861 tgaaagaaat cttattctaa cttgaaattt cacaaaattc caaaaagcga gatttttttt
     4921 aagtatagtt gattttaaat taacaattta tgattaaatt aatgtcataa ttggttagta
     4981 taatcacata taaatataga gcgtagtttg attaaggtta taaatttctc gacgtatatt
     5041 caaatacctt attatagatg ttgccgagat tttaattaat aaatgaaaat aaaatattta
     5101 taaaatactg aggaggctat tatggattta aattctaaaa aatatcagat gcttaaagag
     5161 ctatatgtaa gcttcgctga aaatgaagtt aaacctttag caacagaact tgatgaagaa
     5221 gaaagatttc cttatgaaac agtggaaaaa atggcaaaag caggaatgat gggtatacca
     5281 tatccaaaag aatatggtgg agaaggtgga gacactgtag gatatataat ggcagttgaa
     5341 gaattgtcta gagtttgtgg tactacagga gttatattat cagctcatac atctcttggc
     5401 tcatggccta tatatcaata tggtaatgaa gaacaaaaac aaaaattctt aaaaccacta
     5461 gcaagtggag ataaattagg agcatttggt cttactgagc ctaatgctgg tacagatgcg
     5521 tctggccaac aaacaactgc tgttttagac ggggatgaat acatacttaa tggctcaaaa
     5581 atatttataa caaatgcaat agctggtgac atatatgtag taatggcaat gactgataaa
     5641 tctaagggga acaaaggaat atcagcattt atagttgaaa aaggaactcc tgggtttagc
     5701 tttggagtta aagaaaagaa aatgggtata agaggttcag ctactagtga attaatattt
     5761 gaggattgca gaatacctaa agaaaattta cttggaaaag aaggtcaagg atttaagata
     5821 gcaatgtcta ctcttgatgg tggtagaatt ggtatagcag cacaagcttt aggtttagca
     5881 caaggtgctc ttgatgaaac tgttaaatat gtaaaagaaa gagtacaatt tggtagacca
     5941 ttatcaaaat tccaaaatac ccaattccaa ttagctgata tggaagttaa agtacaagcg
     6001 gctagacacc ttgtatatca agcagctata aataaagact taggaaaacc ttatggagta
     6061 gaagcagcaa tggcaaaatt atttgcagct gaaacagcta tggaagttac tacgaaagct
     6121 gtacaacttc atggaggata tggatacact cgtgactatc cagtagaaag aatgatgaga
     6181 gatgctaaga taactgaaat atatgaagga actagtgaag ttcaaagaat ggttatttca
     6241 ggaaaattat taaaatagat aaatattaaa atatacacaa taggtcgata ttaaaataga
     6301 tatggaggaa ggatttatga atatagtcgt ttgtataaaa caagttccag atacaacaga
     6361 agttaaacta gatcctaata caggtacttt aattagagat ggagtaccaa gtataataaa
     6421 ccctgatgat aaagcaggtt tagaagaagc tataaaatta aaagaagaaa tgggtgctca
     6481 tgtaactgtt ataacaatgg gacctcctca agcagatatg gctttaaaag aagctttagc
     6541 aatgggtgca gatagaggta tattattaac agatagagca tttgcaggtg ctgatacttg
     6601 ggcaacttca tcagcattag caggagcatt aaaaaatata gattttgata ttataatagc
     6661 tggaagacag gcgatagatg gagatactgc acaagttgga cctcaaatag ctgaacattt
     6721 aaatcttcca tcaataacat atgctgaaga aataaaaact gaaggtgaat atgtattagt
     6781 aaaaagacaa tttgaagatt gttgccatga cttaaaagtt aaaatgccat gccttataac
     6841 aactcttaaa gatatgaaca caccaagata catgaaagtt ggaagaatat atgatgcttt
     6901 cgaaaatgat gtagtagaaa catggactgt aaaagatata gaagttgacc cttctaattt
     6961 aggtcttaaa ggttctccaa ctagtgtatt taaatcattt acaaaatcag ttaaaccagc
     7021 tggtacaata tacaatgaag atgcgaaaac atcagctgga attatcatag ataaattaaa
     7081 agagaagtat atcatataat ttaagggggg ataaagaatg ggtaacgttt tagtagtaat
     7141 agaacaaaga gaaaatgtaa ttcaaactgt ttctttagaa ttactaggaa aggctacaga
     7201 aatagcaaaa gattatgata caaaagtttc tgcattactt ttaggtagta aggtagaagg
     7261 tttaatagat acattagcac actatggtgc agatgaggta atagtagtag atgatgaagc
     7321 tttagcagtg tatacaactg aaccatatac aaaagcagct tatgaagcaa taaaagcagc
     7381 tgaccctata gttgtattat ttggtgcaac ttcaataggt agagatttag cgcctagagt
     7441 ttctgctaga atacatacag gtcttactgc tgactgtaca ggtcttgcag tagctgaaga
     7501 tacaaaatta ttattaatga caagacctgc ctttggtgga aatataatgg caacaatagt
     7561 ttgtaaagat ttcagacctc aaatgtctac agttagacca ggggttatga agaaaaatga
     7621 acctgatgaa actaaagaag ctgtaattaa ccgtttcaag gtagaattta atgatgctga
     7681 taaattagtt caagttgtac aagtaataaa agaagctaaa aaacaagtta aaatagaaga
     7741 tgctaagata ttagtttctg ctggacgtgg aatgggtgga aaagaaaact tagacatact
     7801 ttatgaatta gctgaaatta taggtggaga agtttctggt tctcgtgcca ctatagatgc
     7861 aggttggtta gataaagcaa gacaagttgg tcaaactggt aaaactgtaa gaccagacct
     7921 ttatatagca tgtggtatat ctggagcaat acaacatata gctggtatgg aagatgctga
     7981 gtttatagtt gctataaata aaaatccaga agctccaata tttaaatatg ctgatgttgg
     8041 tatagttgga gatgttcata aagtgcttcc agaacttatc agtcagttaa gtgttgcaaa
     8101 agaaaaaggt gaagttttag ctaactaatt tcttggggag ggtattatga gtacaagtga
     8161 tgttaaagtt tatgagaatg tagctgttga agtagatgga aatatatgta cagtgaaaat
     8221 gaatagacct aaagccctta atgcaataaa ttcaaagact ttagaagaac tttatgaagt
     8281 atttgtagat attaataatg atgaaactat tgatgttgta atattgacag gggaaggaaa
     8341 ggcatttgta gctggagcag atattgcata catgaaagat ttagatgctg tagctgctaa
     8401 agattttagt atcttaggag caaaagcttt tggagaaata gaaaatagta aaaaagtagt
     8461 gatagctgct gtaaacggat ttgctttagg tggaggatgt gaacttgcaa tggcatgtga
     8521 tataagaatt gcatctgcta aagctaaatt tggtcagcca gaagtaactc ttggaataac
     8581 tccaggatat ggaggaactc aaagacttac aagattggtt ggaatggcaa aagcaaaaga
     8641 attaatcttt acaggtcaag ttataaaagc tgatgaagct gaaaaaatag ggctagtaaa
     8701 tagagtcgtt gagccagaca ttttaataga agaagttgag aaattagcta agataatagc
     8761 taaaaatgct cagcttgcag ttagatactc taaagaagca atacaacttg gtgctcaaac
     8821 tgatataaat actggaatag atatagaatc taatttattt ggtctttgtt tttcaactaa
     8881 agaccaaaaa gaaggaatgt cagctttcgt tgaaaagaga gaagctaact ttataaaagg
     8941 gtaaaaatct aattaaaaat atatttgctg ttaactagat taaatagtat tttaatctag
     9001 ttaacagaat ataaatacta aaataacagg aggttataac tatgaaatta gctgtaatag
     9061 gtagtggaac tatgggaagt ggtattgtac aaacttttgc aagttgtgga catgatgtat
     9121 gtttaaagag tagaactcaa ggtgctatag ataaatgttt agctttatta gataaaaatt
     9181 taactaagtt agttactaag ggaaaaatgg atgaagctac aaaagcagaa atattaagtc
     9241 atgttagttc aactactaat tatgaagatt taaaagatat ggatttaata atagaagcat
     9301 ctgtagaaga catgaatata aagaaagatg ttttcaagtt actagatgaa ttatgtaaag
     9361 aagatactat cttggcaaca aatacttcat cattatctat aacagaaata gcttcttcta
     9421 ctaagagacc agataaagtt ataggaatgc atttctttaa tccagttcct atgatgaaat
     9481 tagttgaagt tataagtggt cagttaacat caaaagttac ttttgataca gtatttgaat
     9541 tatctaagag tatcaataaa gtaccagtag atgtatctga atctcctgga tttgtagtaa
     9601 atagaatact tatacctatg ataaatgaag ctgttggtat atatgcagat ggtgttgcaa
     9661 gtaaagaaga aatagatgaa gctatgaaat taggagcaaa ccatccaatg ggaccactag
     9721 cattaggtga tttaatcgga ttagatgttg ttttagctat aatgaacgtt ttatatactg
     9781 aatttggaga tactaaatat agacctcatc cacttttagc taaaatggtt agagctaatc
     9841 aattaggaag aaaaactaag ataggattct atgattataa taaataagaa tttgggaatt
     9901 aaaagtttaa ataaaattgt ttaaaaaaca atttcggata tatgaaaaat ctaatttaat
     9961 gggggtaatg aatatgagag aagtagtaat tgccagtgca gctagaacag cagtaggaag
    10021 ttttggagga gcatttaaat cagtttcagc ggtagagtta ggggtaacag cagctaaaga
    10081 agctataaaa agagctaaca taactccaga tatgatagat gaatctcttt tagggggagt
    10141 acttacagca ggtcttggac aaaatatagc aagacaaata gcattaggag caggaatacc
    10201 agtagaaaaa ccagctatga ctataaatat agtttgtggt tctggattaa gatctgtttc
    10261 aatggcatct caacttatag cattaggtga tgctgatata atgttagttg gtggagctga
    10321 aaatatgagt atgtctcctt atttagtacc aagtgcgaga tatggtgcaa gaatgggtga
    10381 tgctgctttt gttgattcaa tgataaaaga tggattatca gacatattta ataactatca
    10441 catgggtatt actgctgaaa acatagcaga gcaatggaat ataactagag aagaacaaga
    10501 tgaattagct cttgcaagtc aaaataaagc tgaaaaagct caagctgaag gaaaatttga
    10561 tgaagaaata gttcctgttg ttataaaagg aagaaaaggt gacactgtag tagataaaga
    10621 tgaatatatt aagcctggca ctacaatgga aaaacttgct aagttaagac ctgcatttaa
    10681 aaaagatgga acagttactg ctggtaatgc atcaggaata aatgatggtg ctgctatgct
    10741 agtagtaatg gctaaagaaa aagctgaaga actaggaata gagcctcttg caactatagt
    10801 ttcttatgga acagctggtg ttgaccctaa aataatggga tatggaccag ttccagcaac
    10861 taaaaaagct ttagaagctg ctaatatgac tattgaagat atagatttag ttgaagctaa
    10921 tgaggcattt gctgcccaat ctgtagctgt aataagagac ttaaatatag atatgaataa
    10981 agttaatgtt aatggtggag caatagctat aggacatcca ataggatgct caggagcaag
    11041 aatacttact acacttttat atgaaatgaa gagaagagat gctaaaactg gtcttgctac
    11101 actttgtata ggtggtggaa tgggaactac tttaatagtt aagagatagt tttagattat
    11161 aatatttaca aattaaatat ttaattatga atttaacctg tatgaataac tatttgttat
    11221 tcttacaggt ttttatagtt aaaaataatt attatctatt aaacaaaaat agtgtaaaat
    11281 atataatatc atgattttga agatataaaa agcaggaaga tataaaagaa agtaggtaaa
    11341 agtttgttta aaaaagagaa tcaaagatat aatcttatat cgtatacaaa tgaagaagaa
    11401 atgactttaa aagaagtttt attggacaag ctaaatttct cagttaggtc attatcaaaa
    11461 atgaagaagg aaaagagtgt tttagtaaat ggagtatata aaaaaccaag tttaaaggtg
    11521 tatagtggtg atttaataga agtaaaaata gacgaggaaa aagcaaattt tgaacctcaa
    11581 gatttaaatt tacagataat ttatgatgat tttgatataa ttatggtgaa taaacctcca
    11641 tttatggtag ttcacccaac aaaaagccac tatgataaaa caatagcaaa tggtattagt
    11701 tactatatag ataatcaaaa agaaaacgta aaaataagat ttgtaaatag gttagatatg
    11761 aatacttcag ggttagttat agtagcaaaa aatgcttatg cacatcatac tttatctaca
    11821 gcaatgagtg aaaataaagt tgaaaaaaag tatataacag tagttgatgg aattataaaa
    11881 gaaaatgagg gaactataga tgagccaatt tatagaccta cagaagattc aataaagagg
    11941 ataatagatg aaagaggaca gtcatctgtt actcactata aagtaataga aagactagaa
    12001 aatgcaactg tattagaagt aagcttagaa actggtagaa cacatcaaat aagagtacat
    12061 atggctcata taggtcatgg aataataggt gatgagttgt atggttatgt agatgaagag
    12121 ctaataaata ggcaagcact tcatgcatac aagctagagt ttgaacaacc aagaacaaaa
    12181 gaaaaattga aatttaaagc agatatacca gaggatatga aagagttaat atcgaaatta
    12241 agataatggg gtgatatata tgataataca taaatttata atacatgttt tggacaaaaa
    12301 tagtgatgtt ccaatattaa atgactttga aggcaaagtt aatcaagaag tagatggttt
    12361 tttccagaaa gctataaaaa gaattgcaaa agatgaggat ttaagaaaag gtgtttttaa
    12421 agattataat gacaatttaa ttaaaaactg ttgtgaacaa ataatatatg atgaaagtac
    12481 ttttttgaaa aattctaaag aaattgcatc ttacttgttt gacgttatga aaattaatgc
    12541 tgcactagaa tcttgtgatt tggcaatttg cttatacact attaaagatg aaaagagtgt
    12601 agcaatactg aaattagatt ataaaaagtt gtacactcat tcaattgaat ttgtggatga
    12661 taaatttaat attcaaatgg tttcaaatga aattggtatc ccagagactt taagacaaaa
    12721 gcaaggagct ttgattagtt taagtggtat aaatgatgag tttcatctaa ggcttttaga
    12781 taaggatgct gaaaaagaag ggtcagaatc taagtttgta acagaatttt taaatgctaa
    12841 aaaaatagat gatgataagt acaagaccaa ggtatttaaa aatacagctg aaaattggat
    12901 aactaatgct cttagtaatg atataaagca agctgaagat gtaagaagta tattaaatta
    12961 tacactgaag gaaaaacatg aagttgatat aaatgacttt gttgataatt caattaaaga
    13021 tgatgagtta aaggatagct ttaaagagca tatggaagaa aaaggtcttg atgaaagttt
    13081 tagtatagat aaaaaatggg ttgagaaaaa gcttaaaaaa agaagtataa aaacggataa
    13141 tggttttgac ataaaaggga atctaactga ttttgaagac ccaatgaagt atactgtaaa
    13201 gcaaaatcaa gatggaacta tagatataat aataaagaat gttacatttt atgaagaaaa
    13261 gtaatttata tacttagtat ataaattaaa tataagagcg tgtattttat atattgtaaa
    13321 taaatgtaat ctagtctgtg cgaactctaa tataatttat tatgtagaag tttacacaga
    13381 ctatttatat ttgtagacaa aacgatacta tttgttttct tcgatatatt tacatatatc
    13441 accaatacat ttaaagttct cagcttcctc gtctggtatt tctattccaa attcatcttc
    13501 aagggccatt ataatttcaa cagcatctaa tgaatcagct tctaagtcgt ccattaaaga
    13561 tgcttccata gttatctcat ctaaattgtc tacccctaat tgttctgcta ttatttcttt
    13621 tattttctca aacattttaa gcctcctaaa gcagttttaa ttaattttaa tgttagttta
    13681 atataatatc aagaattttt caatatggaa tctataaaaa ataataaaat tttataaatg
    13741 tagtaataaa gtattatttg atgaataaac tttattgata acaaatataa cttaggagga
    13801 aatgctatga aatatgtagg actattacta acttcagtag gaatgttttt acttatagca
    13861 gtaaattttt attataattc aataacttta gatatgcaac gtatagagga ttatgtaatg
    13921 gagaccaatt taatccttga agatgtagct gaaaaggaga gttatgtatc taatgaaaaa
    13981 gaagactaca tatcaaggtt aatgcatgta aagaaaggaa tagaaaattc aaaaacatcc
    14041 ttcttgatag aaagatataa ggagtacaaa ataaaatcaa tagaaagttt aatctacact
    14101 atatcagagg agaaaaaaga ttatctggat gaagttgata gatacaataa acttggtgaa
    14161 aaagaaataa ataaactaat caataaaaac tttttagaag taacttatct gtctattact
    14221 acatatattt aattaaagag acaaaactta gtctcttaat gattcaagga ggattagtta
    14281 tgacaaaatt agaagacaat atggaacgtt tctttggagg atgcgaagga ttttttggta
    14341 ataaaatgtt gataataata gttatagtat tcttactatt atgcacagac attttagaag
    14401 acttattatg cgatgacaat atatggatat gggttatatt aatagtattg ttattattta
    14461 acttcgatga tggttgttgt tgctaaaaaa tattgattat taaataggta aaaccagtaa
    14521 cttatacaag ttatggtttt acctttttta gttcaaatta aaaaatttaa aaaaatagta
    14581 aaaatacatt agcaaaatgg aaaccaaatg catagtatat gttaggagcg aaaagctcaa
    14641 caaaatatca acaggaggta acgtacaatg ttagatagat tctttggaga agaaggatgt
    14701 tttggtggag gaggatggtg gataatagta ctattcttcc tattcttagc ttttggagat
    14761 agctgggcag aatttgacat aatggcatgg ataccattcc taatagtttt attaatactt
    14821 tgctgctgtg gtggatgctt agaataagaa cacagttatt aaaaaaagga gggctttgtt
    14881 atgttagata gattttttgg agaagaagga tgctttggtg gaggaggatg gtggataata
    14941 gtactattct tcctattctt agcttttgaa gattgctgga gagacattga cataatgtca
    15001 tggatacctt tcttaatatt attgctaata acttgttcat gtggtggatt ctttgatggt
    15061 gaagtaggtt gcggctgcta atacttaaat cattagaata aaaaaatgct ggtattttat
    15121 accagtattt ttttatttta ttttttaata tgatttaaat taagaatgaa tattattaaa
    15181 tttgttgaat atagggtata atataaaata taatgtattt aaatgtactt gcttttatgg
    15241 taaaataaaa acttatataa atgtagttaa aataggaacc ttcaaaaatt tggttcagtt
    15301 ttagataaat ttagtaaaat aaggaggatg aagatgaaag gcaatataac gataaaagat
    15361 gttgctaaac aagcaggagt gtcaatatct actgtatcta gagttataaa tgattcaaaa
    15421 cctgtaactg atgaagtcaa acaaaaagtt ttagaggtta taaaagagac tggatatata
    15481 ccaaatccac ttgctagaag cttagtaaca aagaagagtc aattaatagg ggtaatagtt
    15541 ccagaagttt cagattcttt tgttaatgag gtgttaaatg ggatagaaga agttgctaaa
    15601 atgtatgact atgatattct tttagcgaat acatactctg ataaggaaca agaacttaag
    15661 agtataaatc tattgagagc aaaacaagtg gaaggtatag ttatgatttc atggatagtt
    15721 gaacaagaac atatcaacta tatacaaaat tgtggaatac cagcgacata tataagtaaa
    15781 actgctagaa attatgatat atatacagta agtactagca acgaagaagc tacttttgat
    15841 atgacagagc atcttataaa gaaaggtcat gaaaagatag cttttataat gacgagtaaa
    15901 gatgatactg ttttagaaat ggaaagactt gctggttatg agaaagcact ttcaaataac
    15961 aatatagaat tagacaagag tttgattaag tatggtggaa ctgattatga gagtggatac
    16021 aatagtatga aagaactatt agatgatgga ataatacctc atgcggcttt tgtaacaggt
    16081 gatgaggctg ccataggtgc tataaatgct atatgtgatg ctggatataa ggttccagaa
    16141 gacatatctg ttgcaggatt taatgatgtt aagatagcta gaatgtatag acctaaactt
    16201 actacagtat atcaacctct atacgatatg ggagcagtag caataagaat ggttataaaa
    16261 ttaataaata aggaattaat tgaaaataag aaaatagaat taccttatag aattgttgat
    16321 agagaaagtg ttacagaaag aaaaaaataa aactattaaa atcaatcagg tagtgcctaa
    16381 ggcactacct gattttttat gtcaataata tcaaatttaa taatagatta actaaaaata
    16441 attatctaat tacttaaata gtttattttt atctactcat gagggttaga ctttttagtt
    16501 ttcttaaata aacctttaga ttttttttgt tgaccacctg gtgtattttt tggtgcaggt
    16561 gaatttgatt tacctttagt aggtttagcc ttcttagttt tagctggttt agtatctctt
    16621 tgattttctg catctgagat tttttccatt atattatcta gttttttgcg ttgattttca
    16681 tctaaaaatg ctttgaattt tgatactact tcatctcttc catctaagtt cttacctatt
    16741 tttataagtt cctccataag ttcattttct gattttcctt tgtaactatc agccatcttt
    16801 tctattttat ccttgtcaat atttttttgt ttagccattt tttctaatga ctctgtatct
    16861 aattttttca taaaaaaacc cctttctata aatatatatt gaattatatt catactattt
    16921 atatatagaa ataatattga agtggtgaat tgtgagtgat atataatata aataaaaatt
    16981 cttatagata atatatatgc actgagcttt aaaaatggaa cattaaaata tgaaaaacat
    17041 actttaatat agggcaatta tataatataa gggggaattg tcttgaataa cttactattt
    17101 ttgattggag tgattgcatt gagtaatggt aatatctcta tatttgatga taaaaataaa
    17161 tcaaaaggaa ataatagagt tagaaaaaac aaaaaaaatg caaatgataa actttcacag
    17221 tctacacatg gcaatttaag gtttgatgca aatgatataa aaagaggttt aaggctcatg
    17281 gatttaagtg aagaagattt agaaatggga atggaaataa taacaagaac taaaaaatac
    17341 atgtcaagag atgaaagaaa aatactaata aaaatagaaa gtgttttaga tttagttaga
    17401 ggaataaaaa aattaaataa tatagatatg gtagatacag aagatgaaac ggattttttc
    17461 agaaaaatgg atagtgatga taaaaagaat atgatgataa aggaaattat agatgtattc
    17521 ccagaaaaaa gaaaaaattc agttgaaaaa gctataggaa tgaaaaagaa gatagattta
    17581 tttgcagaat tatttttacc agatgacttt ggtgaaggtg gatttagttt gagctctttg
    17641 gcaaatataa acaatcttgg tagtatgaat aatctcaaat tgttaggaag tcttttaaga
    17701 ggtgatgata gtaataatga tgatgaagac aataatgatg agttaaatta tgaagacgaa
    17761 gaattagatt gtgaagacaa agaattggat gatgatgagt taacagatga aggtgaaatg
    17821 gaggataatg aagatgaatt agtagaagat aagaacacag gctatgaaaa tatagaaaat
    17881 gatgaagata tagtatacga tgaggaacta tacaacatta atgagcaaga atccgaatac
    17941 aattataatg aaaaaaatac taaaactaga gctaagaaaa aacgataatt aactaaatac
    18001 ataaagaaaa aaggaagaaa aagtaatgat agaataagat tatctcaatt actatataaa
    18061 accactttaa aaaattcaaa atttattata taataataat atgaaatcct gaaacatacg
    18121 ataaggcttt tttaaattca acctacaaat gaaatacggg agaccgtgtc tggtaaatta
    18181 taacatgcct agttagtttg gacgttaaag gttactggca agtcacccac ctgggagtgc
    18241 ctagggtgtg aatttactta gccaccggtg tttcgggaca aaaaaagtgc tactatttca
    18301 aatagtagta ctttttttat taatatgcat ttttctacta aatacgccta attattgata
    18361 aattcaaaaa gcaagtaaca attaagatta taaaagcata gtattaatta ggcttcaaaa
    18421 agccaaagac ttaataagaa tcgcttacag ggagggcaat gaaaaatgca agattataaa
    18481 aaaaataaaa gaagaatgat gaatcagcca atgtctacaa tgaatgaaga agaagtgtat
    18541 acagatgaaa taaattcaga agacatgaga gggtttaaaa aatcacacca tcataatgga
    18601 tgtaatactg ataataagtg tgagtgccat gatgagtgta atccatgtaa cccatgtaat
    18661 ccatgtaaac ctaacccatg caatccatgc aaacctaatc catgtgatga caaatgtgaa
    18721 tgccatgaca agtgtaaatg tgattgtgaa ccatgtgaaa tggattcaga tgaatgtttt
    18781 gaaaacaaat gtggaccaga atgttgtaat ccgatatctc caagaaactt ctctgtatca
    18841 aatgcagtgc catttgcaat agaggctaat agaatatttg atactatgca attccaaaca
    18901 tttacagatg caacaggtcc aaatggagaa ccattaactt ttgaaacaga agtagtagaa
    18961 gtatttggtt caattccaag tgcaggtcaa gcaagtgtaa ctatagaaaa aatatgctta
    19021 agtaatgatg gaatagttat agacacagga atgacaactt tagaagattt cgatttagac
    19081 ccattaggag atatagtagg aagaaactgt gaaacaactt ttgaatttgc agtttgtgga
    19141 gaaagaaacg ctgagtgctg tagacaagga aaaggcaaat cagtagctta taaacaaaga
    19201 ggattaactg tagcagttcg taatttagta ctagagctaa gaggtagatg tggatgtaca
    19261 gagttcgttg cattagcttt cccagcagtt agagcaggag gtggatgtaa gagaagagtt
    19321 gattatgtag aatttacttt taacacactt tcagcaccaa tatgcttgcc agctgacgga
    19381 agagctgtta ctttaagaca agaatatcaa actaacttaa ctgtagattg tataggaaaa
    19441 tctatattaa aattagaatg caacgaatgt tgtgaacctt tctatgaatt aattatacca
    19501 aatgatatag atttagtact ttgcttacaa gaaacagtta gcacattaat aagtgaacaa
    19561 atagtagttt tagcatcacc aaatccaatc caaccaagac ttgttgatac tttctctaaa
    19621 gtatgtgatt tttcgcaatg tggacctaat catggaagtg gaaagccaag ttgccacaga
    19681 tagataggta agatatatag acagtttaaa ttataaataa aaatcatcta aggaaggcta
    19741 ccaaagtgta taatttacat tggtagcctt cacgttttta taactagcgt atagatttta
    19801 gtgatttaaa aagcactata gtatgtaata gacaagtaaa gattgatact atcgtcattg
    19861 tgtatatgta tgcataaata ttaagtcctg gaattggaag aaatacatat aaagctatta
    19921 gttgaattaa catgccagta atagtattta tacttgaagc taattctttt cttattgaat
    19981 gtaaaatgcc agatagagtt tgatttaggg acataaatag tggaactaaa aacatagctt
    20041 ttagatattc tcctgcaagt gtatttttaa atactaatat acataaatct tttccaaaga
    20101 aataaaaaaa tattgatgat agtatgccta caaataaagt aagaagtatg gagtatttga
    20161 tttttttaat tacactctta cgctttctta gagccatttc ctgtgaaata gttggtatta
    20221 aatttacaat aagcgctgaa ccaacagtaa aaggtaaaaa tataaagggc attaccatcc
    20281 cactgacaat accatacata cttaaagatt gttgataagt cattcctgat aatgctaatc
    20341 ttgatggtat catcatagaa cttatagatt gcaaaagtat gtttgacatt ctattacaag
    20401 ttatgggaat agacattttt aatgtttcca tagaagaatt ataaaaatct tttatgctta
    20461 taatatacct attgtcaaat gatgaatctc gccataagca aataagcata aatataatat
    20521 tgctcaactc accaatagat atacctaata gagctatata acaattcata gatttattat
    20581 ttatgtacat aacaagtaaa aaaacaaata atattttacc aatttgttca atgatttgtc
    20641 caatagcagg gatttttaca ttctttatgc catagtaata tcctcttagt acgtttgaaa
    20701 gagttattat aactatagca ggacaaacag ctagtataaa tagatttaat ttagcatctt
    20761 ttaaaaattt aagtgataaa aaagaactat tgaaagatgc aactatagat ataattagtg
    20821 ctacgaaaaa tgatatatat aaagttgata taaatagagc attagtattg tgtctatcgt
    20881 ttaaagcttt tctctttgca actagacaac taagtgcagt aggtatacct gttgttgtaa
    20941 cagctaagca aatcatcaaa aagttaaaaa tcatatgata gatacctaaa cctgtttcac
    21001 ctagtgctct tgataagaat attttatata aaaaaccaaa tattcttaca ataaaattgg
    21061 atataaataa aataactgtt gatagtacta aataaggtac tttcaaaaaa atcacctcct
    21121 tatcttttaa atatattcac aaggaggtgt aaaaatttat aattttataa attctggttt
    21181 caatttatta aatttagata catatttaaa tcccatattt tttagtaatg aatatatgta
    21241 gtcaaactca catgcaactt gacttatatg atgtgaatct gaacctgtgg ttatgatttc
    21301 ccctcccaag tctttgtaca tttgaagtat atacttagat ggggttaggt ctggaagatt
    21361 atatctataa caagaggtat ttatctcgat acctttgcca tcataaatag cttgtttaag
    21421 agtttcttca attatatcag aaaatagtct atcatctaaa atagtgtcat aaggtgcata
    21481 ccttttaatc aaatctaaat gacctaagac agagtaatct ttataattct taacaatatt
    21541 atataaataa agatagtaat tttcataaac ttcatattga gttttatctt taaaataatt
    21601 accaaggtat aagtccattg tatctatagc atggatagaa catattataa agtccaatgg
    21661 atattgatgt gcttcctttt tgtatgtatc cattaattga gtttgaactc caaattcaat
    21721 tccttttttt attgatattt tatctttgta tttgctttga agagtctcta aactcttaaa
    21781 ataatcttcg tagacaattg aaaaagaatc atcagcatat acatcatagt caacatggtc
    21841 tgtgaaacaa atttcctcca aaccgagttc aatagatttt tttaccattt cttccattgt
    21901 agattttccg tctgtagaga aacttgagtg catgtgatag tcgtaaaaca taaatatttc
    21961 ccctttttat aaaaattttg gtattatata tacatactaa ataatatcaa atatatataa
    22021 aagcatcaat aaaatataaa tacaatgagc ggtgataaat ttggaaaaat acagaataga
    22081 tttaaatgag aaaaaatcaa ctaaatatat acagatattt aatcatataa aagaattgat
    22141 aataaataaa gagttagtag aacatgaaaa attgcctcca ataagaaaac tatcaaattt
    22201 tttacaagta aataacacta ctatagttaa ggtatacgag cttttagaaa atgaaggata
    22261 tgtatataaa attataggga gtggtacttt tgtatcaagt tcaaattctt ataaaagcgg
    22321 tatagataac aggaaaaata agattattgt gcaaagagaa gatttaattc attttgacaa
    22381 tgggaatcca tctaatgata tgtttcctat cgattcattt aaaaatgctg taaatatggc
    22441 attatctaaa gatggtagtt caatatttga atatgatgaa ggtcttggct ctgaagaatt
    22501 aagagaaaaa atggtagagt atctaagtga tggaaatata aaaactacta aggaaaatat
    22561 tcaaataata tcaggtgctc aacaaggtat agatatagtt tgtaaaggac ttataagtta
    22621 ttcagatgtt gtttttatgg aagaaccttc ttacaatgga gcaattgaag tgtttaaaag
    22681 tagaggtgca aaaataatat ctatacctat gttagatgat ggaattgata taggtatttt
    22741 aaaattaaag ttggaaaaaa taaaaccaag acttatatac attatgccta attttcaaaa
    22801 tccaactgga atatcttact caacatataa aaaaaagaaa ttgatagaat tggcagaaga
    22861 acatgatttt tatataatag aagatgattt tataagtgat tttgtttttg aatcaaagga
    22921 caatagaaca attagaagtt atgatgataa aaatagagta gtatatataa aaagcttttc
    22981 aaaaatactt atgccaggtc ttaggatagg aattgtagaa atgccaaatg agttgttaaa
    23041 aaaagtttta tgggctaaat attcatcaga tatatcaaca ccaggtttaa ttcaaaaatc
    23101 aatgtattac tatatgaata attttgattg gaagaattat ttgagtcata tagagaagat
    23161 atatactaat aagtataaat tgtcaaaaaa cttgattaat gacaaattga gtggtaaatt
    23221 aaaagttaga aaatcttgtg gaggaataaa ctttttttta gaattaccaa gaggatatac
    23281 atctcaagca tttacaaatt ttatgttaaa caaaggagtg tcaatgcttc caggaactta
    23341 tttttttgac aatctagtgg atgatagatt ctttaggata aatatagctc gcccaagcat
    23401 ggaagaacta gaaaagggaa tatctgtaat aagtgataat atagatgagt ttttttgtga
    23461 atataaaaac aagttagata taaaaagcaa taaattattt tattaaataa atagaggttt
    23521 tttaggagga aaaaatgttt gacaagaaga aattagatag gattaatgaa ttagcaaaga
    23581 aaaataaaga gggaatcctt tctgctgatg aaatcaaaga aagagaaatt ttaaggaaag
    23641 aatatttaga aaactttaga gctcacttta gatcaagact agatagtgtc aaagtagttt
    23701 caccagaaga atatgagcaa tacatgaaaa acaataaaaa ttagaactta gtaggaggaa
    23761 aatatgaact taaagtacga atttaagaat gcttgggatt ttgaaagaaa agaaaatact
    23821 attgaacaaa ttatgcaata ttctcgcaat tatatggagt ttttaagtaa gtcaaagact
    23881 gaaagacttt cagtaaaaga aattataaaa ttggctaaag aaaataacta tatttcaata
    23941 gatgaagcta tggaaaaagg aagcataagt tgtggtgata agatttatgt tataaataaa
    24001 gagaaggcag tagcactttt tgtaataggt aaaaattata tagaaaaagg tatgaagatt
    24061 ataggaagcc atatagattc accaagatta gatttaaaac caaatccact gtatcaagag
    24121 agtaatctag gtttttttaa gactcattat tatggtggaa taaaaaaata ccagtggaca
    24181 gctcttccac ttgctttaca tggtatagtt atattaaatg atggaacaaa agtggacata
    24241 tctattgggg aagaagatag tgacccagta ttttgtgtaa ctgatttatt gattcatttg
    24301 gctggagacc aaatgcaaaa gaaactatca gaaggaatta gtggagaagc tttaaatata
    24361 ttaattggga atattccttt agaagatgaa gaaaaagaac ctataacagc aaatatatta
    24421 aagattttaa atgagaaata caatatagtt gaagaagact tattaagtgc tgaaattgaa
    24481 gtagttccag ctggtaaggc tagagattta gggtttgata ggtctatggt gttgggatat
    24541 gggcatgatg atagggtatg ttcatatgct gctgtaaaag ctatactaga aactgaacaa
    24601 ccagaattta cttcagttgc attatgtgta gataaagaag aaattggttc taaagggaat
    24661 acaggtatgc actcaaagtt ttttgaaaat actgtggctg aactgattgc tttagaagga
    24721 gattattgtg atattaaagt aagaagagct ttagcaaata gtaaggtatt atcagctgat
    24781 gtttctgctg gatatgaccc aaattttgga gaagcatatg aaaagagaaa ttcagcatat
    24841 atgggaaatg gagtagtttt aactaagtat acaggttcaa gaggaaagag tggatgtaat
    24901 gatgctaatg ctgaatttat gtcagaagta aagagaattt ttaataaagg aaatgtagta
    24961 tggcaaactg ctgaacttgg aaaagtagac caaggaggag gcggaactat agcccatata
    25021 ttggcaaatc aaggtgcaga ggttatagac tgtggtgttg gagttttaaa tatgcatgca
    25081 ccacatgaga ttgtctctaa agtagacata tatgaaatgt ataagggata taaggcattt
    25141 tttaatataa atttataagt aaaaatataa atgtaataag tgaataaagg ctgttgtata
    25201 aacaatttaa tctgttgtac aacagtctta tttttatgta aaaatagcaa tttgtcacac
    25261 tagagaaaat aaaagaaaat actgtcaaat taaaaattat ttaaaaaaaa attgacaacg
    25321 atttcatctt gttataatat gtaataaaga acttgttata taatgttata aaaatgttac
    25381 atagggaggt gagcaaaatg gatgaaaaga gacaaaaaaa agaaatggat ttagaagtaa
    25441 attcctttgt tcctttatat caacaactat atgacaacat aaaaaaacaa atagcatctg
    25501 gcatatataa accaggagat aaacttccat ctgaaggaga cttatgtaaa gaatttaata
    25561 taagtcgtat aactgtgaga aatacgactt ttttatataa aatagcaaaa taacagtgtt
    25621 tgagcaaaaa aaaagaatgt tttttatcgt tttgccacct gtttgccacc ataacttttt
    25681 tcggtggcaa attattgtaa aatgccctcc aaaatatcta ttgtttcact ttccatttta
    25741 cttgtaacat gtgaatatgt gtccatagtg gttgataatt gactatgacc taatctttgt
    25801 tgtatatatt ttacattagc tccattttct aataataatg tggcatgtgt atgtcgcaag
    25861 caatgaaaat taaagttaat atttagtttc ttgttaatag ttcttatagc agcgtcaata
    25921 ttgttgtgat ttacaaatga gccatctttt tttctacaaa cccaatcata ctcagtttct
    25981 ttataccatt ttccaatttt aattttttgt tttttttgat tcaatttttc ctcttttaat
    26041 atcctagaca aagtatcacc tattttaata tctcttattg atgtttttgt cttaggtgat
    26101 gctaattcaa attctgaaac ttttctcttt attaaatttt ttctaacttt gattatatta
    26161 ttatctaaat caacattatc ccaacaaaga cctaatattt ctccttttct catgcctgta
    26221 tgaaacccta taagtagagg aatatagata tttgtatttt taggatatat ttctagtatt
    26281 ttattgaact catctagtgt tatagtcttg ttatctgact cattttttac ctttaaaaca
    26341 ttttttggta tactggcata ttgaacagga ttttccttaa tgagtttgta agggtaaaca
    26401 gcagatttta aagcagcatt taatacaaca taaatcgctt ttaaaactcc ttttgtgtag
    26461 tgtttttctt ctccgttttg tgtgtatgtc tcttttgatt tattatttaa aaattcttga
    26521 ataatagctg gatttataga ttttagctta catttaccaa gtctaggttc tatatgattt
    26581 tcaattaaat ttctataact ttcctgagta ttgtatttgc aattaagaag tacatactct
    26641 ttgtaccaaa agttaagata gtctgataaa ctcatatttg tttcatcaaa cactatacca
    26701 gcattttcat attcatttat tgcatcacgg agcgattttt cagcttcttt cttagtgttt
    26761 ccaccaactc tttctacctt ttttctcttg ccatctacta tacctgcatc aaagtagtaa
    26821 taccacttct ttccacgttt tcttacgccg cctttcataa aatctctcct ctcaaaagct
    26881 ttttttatgt aattatataa taacatatat caatttaaat aaaataaata aaaaagacta
    26941 taagaaacaa agttttatgt ttataattaa tagtcttttt tatttagata catatttaat
    27001 gttttttatc acgtgattcc agcacttttt ctgccaaata aacaactatt ttttcatact
    27061 caatatcagt ttctaaagaa aagtcaacaa tagcaggtat cgtcatatct cctgcatatt
    27121 tattacctaa ccaattttca attaattcca attcttcttt gcttaattca aattttttct
    27181 ttttattaaa acttaagttc aatttattca cctcaaataa tattatatat taaaaatagt
    27241 atttgataga aacattcgaa ttatttatgt aattttcttt ttttaatata ttcatcagca
    27301 aaataattta ctaaatattt ttcctccaat cctaaagcta tttctaattc aataagagca
    27361 gggatagtta aatctttatt ctcattattt tccaacctag aaatttgact tctgtggcaa
    27421 ccaactcttt ttgctaactc tatttgtgtt aatttcttct tttttcgtaa ctcttttaac
    27481 atatatttaa cctacctttt agataatttt atatatgtta gtttgtgtaa tttcttgtaa
    27541 taaatgtgca atattcgcac actttttgtt ggaaatttgt gctagaatgt agttaagaaa
    27601 taactttatc tagataaagc aaaaataata gaagctataa aatataataa tattttacag
    27661 ttattttata gttttaagct ttgaaaaaga ggtggttgta actattttta agaacgtatg
    27721 tttcgtggaa ttaataaata aaatatttta taggggatga tatatttgaa taagacgaaa
    27781 tattatgata ttttaaagtt aaacttatta atgaaaaagt tgaaagaatt ggataaaaat
    27841 aaaatcaatg aatataaaat aaaagtgaaa gaaatacata aaatcaataa aaaagaggaa
    27901 tgattcctct tttttattat attcattttc agaaataaac tattttttat ttttttcgtc
    27961 tagtaaaaat aattctgcaa cttttaatgc tttttctctt gcgtctgggc ttagctcgct
    28021 aaatatatta aaaacttctt tcatatcttc gtcaagatac atattctcaa taagctcctt
    28081 ttctgtttta aaattttcag catcataatc tttttcgttt ttatttataa ataagctatt
    28141 tctaacatca gttcttccta ataaataatc tgtagacaca tcaaaatagt ttgcatattc
    28201 ttctattgta cttttcttag gttctcttaa accattttct atccttgata gtgtagattt
    28261 gtttatatgt aaatcctcgc ttaatttgtc tagcgagatg cctttttctt ctctcaattc
    28321 ttttatccta ttcaatttgc acaacccctt tttatattaa ttccaaaata gcaactttta
    28381 tttatattat agcaacaaaa ttaattatta caattttttt tgctaaaaaa gcaacaaaag
    28441 tattgactat aatcacaaaa cttgatatta tataaatatg aagttgccaa aatagcaaaa
    28501 aggaagtgat ttaatgtact taaatagatt agaaggattg atgaaggaaa atagacacac
    28561 ccaaaaaaat gtggcagata tattaggtct tagctcatat ggttttagat taaaattaaa
    28621 aggaaaaaac gaatttaaag caagtgaaat aaaaaagata tctaaattat ataatgtatc
    28681 tgcggattat tttttttcag atgaagttgc taaaatagca ataaaagaag aaaggggcaa
    28741 taaataatga agaatctaac cataatcaag caaaataatc aatttttagt tgaaagtaga
    28801 gaagtagcag aattaataga aaagaagcac gataatttat taagagatat aagaggatac
    28861 aagaagattt tagaggactc atcaaatttg aagagtcaag atttcttcat agaaagtact
    28921 tatataaata ctcaaaataa aattcaacct tgctacttat taactaaaaa aggttgtgac
    28981 atggtagcta acaaaatgac aggagaaaaa gggattatat ttacagcgat ttatgtaact
    29041 aagtttgaag aaatggagcg agagttaaaa gaacagcaac ctaaactacc aactacgtac
    29101 aaagaagcat tgcaacagtt attaatagaa gttgaagaaa aagagcaatt acaattagaa
    29161 aatcaagaaa aagataaggt aatccagtta caacaaccaa aagtactgtt tgctgattcg
    29221 gtagcatctt ctgacaattc aatcctaatt ggagaattag caaaattgct tagacaaaat
    29281 ggaattgata caggacaaaa tagattattt gactggttaa gaaataatgg ttacttaata
    29341 aaacgtaaag gtgaggatta caatacacca actcaaaaaa gtgtagattt aggagttata
    29401 gaaacaaaag aaggtacaag agtacatcca gatggtcata caagtattac taaaacacct
    29461 aagattactg gtaagggaca aatatacttt attaataagt ttaaaaagaa caatcaaata
    29521 tcaatgttag gttaaagatt aatagcactt tgaaaactaa atacagaata ttttgaaaag
    29581 gaagtgatta catgagtaat aaaaagaaat atactttgag tattacagaa gaattatata
    29641 ataaatgcaa agaaaaagct aatcaaaaag gtatgtcagt aaatgagtac atactattag
    29701 tgattagcaa gaatttaaaa aatatttaat ttttattaac ttaaacatta attttgccat
    29761 ttattttttc ataactttca acatgttttt taattaattg ttctatttct ttgttggcag
    29821 aacgaccttc actttcagct atatacttga ttttttcaag tagattgtta tctattctaa
    29881 gtgtgtatct cggtaatttt gatggcataa atatagcacc ctccttaagt ctaaatgatg
    29941 tcactattat accacgtaaa ttatttcaaa aaaagtgttg acgcaaaggt gacgcaatga
    30001 taatataata aacaaggagg tggttcaaaa gtgacggcaa agaagagagt aactgttaga
    30061 atgccagacg aattaaatga agagctacat agacaatctc aaagaaaagg attaagtaaa
    30121 aatgctttta taataaatat cttatggaaa gaatttgagg atttacagga cttaaaagat
    30181 gaacaggagg ttgataagta tgaataactt acaaatattt gaaaagatgg agtttggaca
    30241 aataagaatg gcagagattg ataataagcc ttactttgta ggaaaagata ttgcaaagtc
    30301 attaggttat aaaaatacca atgatgcaat tttaagacat tgcaaagggg tagtgaaaca
    30361 cgaggggttt aaaattaatg gtattaagat tgctttgata actgagggtg atgtttacag
    30421 attaatagta ggttcaaatc ttccaaacgc agaaaagttt gagagttggg tatttgatga
    30481 agtgcttcca actattcgac aaactggtca atatcaagca caacaaaatg taataacaga
    30541 acttacagga actataggag atttaaaagg aacaataaaa gaatataaaa agttgtgtaa
    30601 gataacatgc tctaagaaac aacagtattc taaatacata aagaatagac tggatataga
    30661 caaagctaat aaagaataca accaagtgaa agcaaggcta ttcctaattc tcggagtgga
    30721 gaaatgggaa gatatagact ttgacacatc taataattta atacaaatta tagatgattg
    30781 tataaaggtt ataaagtcag aaagaccata caagcagttg agttattttg agaactaaat
    30841 acagaatatt ttgaaatata ttgttttaat taattattaa ctaggaggtt aaatacatga
    30901 aaaataatac aagcgattca agagtaaaat atttttgcaa atgtccatac tgcgggtttg
    30961 ataatgaggt agaagttaaa aaagggttga agcctaagat atgttgcata tgtacaaaag
    31021 aagttgagta tgaaaaactg gagcaacaaa gtgctttgga aaataccgaa attaaaggag
    31081 tgtgtaatca aatgaaaatt aaaaaattag cactagcaac aggagaagta tttgaaaaca
    31141 tagagctcaa agaatttgaa aataaaggag aatgcttacc atcaaatatg gtaaaggtta
    31201 ttgatggtaa gcaggaactt ctaataaata aagagttcat attatcatta gaagtcaaaa
    31261 gaaagactac tttttaggtc ctttcttagt ttgtgacaga gcactaccag caaccgattt
    31321 ggaagcagca ctatatcttc cgtctttaag aatcttacta gccttagaag caacagtttt
    31381 acttgtttgt ttagtatttt tagctatagg tatcaccacc tttaaatgta tttataggat
    31441 atatcctaca aatatagtat atcaaaggag gaaaataatg gcaattaatg acaacataaa
    31501 taaaatttta aaagatagag atttaaaagc atggaaatta gcaaaagaaa taggtgtaga
    31561 ttcagggaat ttatatgcaa ttttaagagg agaaaataaa aatccaacta tagatacatt
    31621 aataaaaata gccgactatt tagacattac attagatgaa ctagttggaa gataaaaatt
    31681 aaatacagaa tattttgaaa aggagtgagt aaattgggca atatatctaa tttcaattta
    31741 gataaacaag aagataaaag ttttaatgac ctagataata tatcaatttg tttttcagaa
    31801 ggtatccgta aagttgtaga aatgaattta aacaactata aaaataaaat ctcaaagtac
    31861 ttaaatgaaa cttcgaaaat agaattatta gaaccaaaag aactaacaat tgttataagt
    31921 aaaggttatc ccgattatct tatgtctgtt gaagaagcaa gtaaaagatt gaaaatagat
    31981 aaagtatttg gatatgagtt aataaaaaat gggcttttga agtcagttga tataggggca
    32041 actaaagttt ctagttatga attagatgat tttataacta ggaatcaagg aaaaaacatc
    32101 aaagaaatgc ttagagaaat gaaagaactt agagaggggg tgatttaatt ggaactagta
    32161 acatacagaa acaagcttgt tttactaaaa gatggagaaa agattgcaac tataagttta
    32221 aaaaggaaat ttctcagcaa cagacttaaa ttaaaaataa gataggagag ataaattgaa
    32281 aataatttat aaaaacaaag tttacaaagt agaacaagac aaaaagttat ttagaattac
    32341 atactatgat gagcagagag gtagtaagaa gtttaataaa gagaagaaaa taaaaagaag
    32401 tgttttaaca agagatatag agttagttaa cttgtattta ccagcacatt taaaaataaa
    32461 ataagctata aataattaaa gaaaaaggtg attagatgga aatagaacaa gcaacaatac
    32521 gcctgcctag agaacttaaa gacaagcttc taaaacaggc aaaagttaaa ggatatacat
    32581 taaaagatat gatagttttt attctaaagg attatcttca aaatatttct caagaataaa
    32641 ttcaatttct ctacctatag aacgcttatc tttttgagca agttgttcga ttttctcaaa
    32701 aagaagttta ttaattctta gtgtaaatct tttatcctct tcacgagtat aaatatcttt
    32761 attagacata tttatcattc ctcacataaa atttgacgtc tttatgacac tttaataata
    32821 aaataatata aaagaaaagt caacaaaaat gcttgacgtc aaatatatga catggtatta
    32881 ttaaaacaag aagggagttg acgtcaaata tatgacaaat gaaagagtta gatttacatt
    32941 tagattacca gcaccattgc ttgaaaaaat taaaaataga gcatcaatag aaggtagttc
    33001 aatgaactca cttatattac acatactttg ggattacata aaagaaatag aaaataagga
    33061 ggtcaaataa tggttgaatt ggtaaaagaa tttgatttac aaacaattaa agtaggaaat
    33121 gcagtaaaag taaattgcaa aagatttggt tttgaaattg attgtatagt agtagtagca
    33181 acagaaaaag aattaaattt agcatacttt gatgaaggta gaggctgtat ggagtatcaa
    33241 gccttaataa cagaagatat tcaagatggt gattatgaga ttaaaatttt atcttaggag
    33301 gaaataaaat ggctgcttta ataatggtag gtttatttgc aatatgttta gtaggattag
    33361 ttcaaaatag agattaaatt aagggggatt aattatggaa agtttaaaaa gaataaaaaa
    33421 aatggttcaa aaacaattag ttttagctga attagagata aacaaaaata gcaaacttta
    33481 tgaggaactt gaaaataagg atagaggttt aatagatgat atacacatga gagaatatct
    33541 aagagaaaaa gttgcatggg aaagagtaaa atatgctatt gaaaatattt taggtggtat
    33601 aaatttagag attaaatcaa aggaacatga agaaagtgag gattacaaga tatttcaatt
    33661 aattttagaa gaacttgaaa gagataaacc tatagatgtt cagatataag aaaagagcca
    33721 ttgccgtggc tcaattcaaa taaatatatc aaaatttaat taagctaatt atagcacaaa
    33781 cggagggaaa ttatgagtac tttatatgaa ttaactacag atttattaga aatagaagaa
    33841 ggtttaacag aaacaacagg aaatgaaact gaaaaactag aggaaataaa agaaataata
    33901 aaacaagaga tacaaaataa aaacactagg atagtttcag taatattaaa cattgacagt
    33961 gatataaact ctatagattc agagattaaa agactgcaag agttaaaaag ggtcaaaaag
    34021 aatactcttg atagattaaa aagcaatata aaagactgta tggaattact tggtactaaa
    34081 aaagtagaaa cagttttagg aaatataagt ataagaaagt cagcaggtag cttagtcata
    34141 gaagatgaag aaaagatacc tgctatatat aaaacagtag agcaagttgt aaaagtagat
    34201 aagaatatca ttaaagactt tattaaaaaa ggtcatgagg ttgaaggttg caggattgaa
    34261 tatggaacta cactaacaat tccaaaagct aaaaaagagt aggtgaggac catggaaact
    34321 aataatgttt atataaaact tgtaaatata cagagtactt taaaagctcc taaaagtcaa
    34381 tttaatagct ttggtaaata caactatagg agttgtgagg atatactaga aggtttaaaa
    34441 cctattctaa aggaagaaaa agcattagtt atattggatg ataatattgt tcagatagga
    34501 aatagatttt atgtagaagc tacagcaact ttaatagatg cagaaacagg agagaaagta
    34561 tctacaaagg cattagctag agaagatgaa actaaaaaag gtatggattt agcacaagta
    34621 actggaagtg tatcaagtta tgcaagaaag tacgctttaa atggattatt ttgtattgat
    34681 gatacaaaag atagtgatgc aacaaataaa catggaaatg agcagaaaaa aaaagaagtt
    34741 aatgagagtg aattaaatac actatattcg ctaggagaat ctatagaaaa agataaaaat
    34801 agagttgata gtgaagtata taagaagttt ggaaagttag cagtagattt gactaagcaa
    34861 gagtatgaga aagttttaaa tggatataag agcattttag agaagcaaaa acaagagtag
    34921 gtgatagtat tggggattat aagagtaagc aaagacaaag ataatccata tgtagtttta
    34981 aataaaactt gtttggaaga tgtaaaatta agctggcaag caaaaggttt acattcatat
    35041 ctgattagta agcccgacca ctggaaaatc tatgttaatg acctttgcaa gagaagtaaa
    35101 aatggaagag atgctacagc aaatatttta aaagaactta tagaaaatgg atatataaca
    35161 agaaaacctt gtagagattc taatactaat aaaatgcttg gagggtatga ttatgaagta
    35221 tatgagatac cacttgaaaa tcctcagaag ctaaaatccc gaaaaactga tttcccggaa
    35281 accggatttc ccggaaaccg ggtttctcgg aaaccggaaa acacggaagt agtaagtaat
    35341 gactttaaag taaataatga tattactact attgttatta atgaacaatc caataaagac
    35401 aaaaccacct acataaaaaa atactttgaa aaatatatag gtgtgattac tcctaataac
    35461 tttatagagt taatgagtta cttagatgat ggaatggaag ctgatgtaat tataagagct
    35521 attgatgaag caatagcaaa tggagttaag aattataagt atgtaaagac aatattaaat
    35581 aattggatag aagcaggtgt aaaaactaat ttagaactta cagagtatca aaatgagttt
    35641 gagaggaaga aaaagaataa acaggataag aagcagtcta atagtaaagc tgtgaatact
    35701 cctaatgtga gtaaaaataa gtttcataac ttcaatgaaa catttactca atattcacct
    35761 gacgaactag atgacataat taagaaaagc caaaaggtta aatttaaata aaattaaact
    35821 tctaggaagt aaatatcaat atattgcttc ctagaaaagg ggaggtataa aatggcgaga
    35881 atatatgcac aaagaagtgg ttctttaaat gaacaagata gattggagtt attaagatta
    35941 cttgggaaag ctggatatac agtaaaggtt gctagagaga agcaaaatag caagacaact
    36001 tatacttact ttgttgagta tacagaagaa caggaagaaa aatagaaggg ggctagttaa
    36061 atgaatacaa taactttagt tggaagatta gttgcagatg cagaattgaa gtaccttcca
    36121 aattcaggta ctccaaaaat aaccttttca atggcagtag atagaaggtt taaagataaa
    36181 aatggaaata aaataactga ttttattcaa tgtgagcaat taggaaaaca tgtagagaat
    36241 ctagtgcaat atcttgttaa agctaagcct gtttatgctg ttggagagtt aaatatatat
    36301 aattacaaag atgaaaatgg ttgctggaaa tctattacta aggttaatgt aaatgcttta
    36361 gaactacttt ctagtaaaaa tgataataat gctaaacaag aatatgtacc accaggatta
    36421 gacccacaag gttttcaagc aatagatgat gacgatatac ctttttaatt aagttaaata
    36481 gtctagggag tgattataca atattacttc ctaggagttg aaaaatattg gaggtctaag
    36541 agtgaaatat gagtgtgaga aagtgttctt agaatgcgat aagggaagtt ttgagataaa
    36601 tgatacaaga attgaagaag taacattcga gggtacagaa atagacaatc cgtttgagag
    36661 agtaaaatat gaagggactt ttacaataat atctggatgg gattctttat tgagagatat
    36721 attgtggctt gaaatgcgga aagtcttaaa aattataaca agaaaaatga tgatgggggt
    36781 attaggaatg ataataatta gaagtcaaga tagattagat ttaatgagag ttaacagagt
    36841 tgaaatatac gataaacaag tatttgtaat atttgaagat gatgtcaaac gaataggtgt
    36901 atatgaaagt aatgaaagag ctattgaagt attaaacaga atacaggagg ctattattgc
    36961 aggaactaag tttgacatta taaataaaga cggggttaga tgcaacaaag aaaaagtgtt
    37021 tgaaatgcca gttgaataag gagggagctg aaatgttaaa ggttgaaaaa tattttaatg
    37081 gttctgttgc agataacata tttgaagatg atttgactct tagaaactac ttagcactat
    37141 attgttgtgt ttatggagta agaaaaaatg gagagcttgt atttccaact tctgaaaaaa
    37201 tgctaataga gtttaatgtt gataaaaata gaaaaaagaa aaagaaaggt tcaaaggtaa
    37261 agttgattaa tgccaaaact ggtgaagaaa aaatatttga ttctatagat agtgcagcgt
    37321 gttttttaag actacagagt caggcagttt accaaacaat taaaaagaaa actaaaacaa
    37381 gaagtggctg gaaagctgaa tatattaagg aggaataatg gaagtttcaa ggactgaata
    37441 cacaattaaa agagcaaaag agttgtatga caatggagag gacatattta ttgctataga
    37501 taaggctaga gaagaatatg aggagatgat taaaagtgag tttaattaag tacagaggtt
    37561 atgattttga gaacgaaaag tggatttatt cagcaacaat aatgtggagt aatatacttg
    37621 aatgtttagt tatgttgaca gagggttgca aatggcagaa agtctctaat gttggggtat
    37681 gctctggaga atgggctaga aacaatcaag aaatttgtga aggggatata ttgaaaggat
    37741 atgataattc tagtgataca agccaatatg gagttgtaaa aagagatttt aatagtatta
    37801 agttatattt ggaatggcat tatttaaaga agtttgaagg agaatggata gagcttataa
    37861 ataaaacaga aatatatcat agtagagatt acaaagtaat tggtaatgaa tatgagaatt
    37921 tagaggaagt cagaacagag ttcttagaac gtaaggagag tcttgaaaat gaatatctta
    37981 gctagtgcga tattagtaat aggaagtttt ataactggta gagtttatga gtatagattg
    38041 aatctaaaag agtgtgaaaa ttgcgacaat aaaagaggtg tataagaatg gatgatagat
    38101 tagaaatgat aaatgcttct gtaaattata tacagatgat atgtgaaagt tcaaatatag
    38161 ctattatagc agagcgagga agagttagaa tattagattt agaaactaaa gaaaaatatg
    38221 atttattaaa aaataaactc gaggaaatgt tagaagaaat ataagtgaaa atatctaatt
    38281 aagacagttt agagagttac aaaatatctt ttagtataaa ttattgttga agtgttttgt
    38341 gactctcaaa aatgaaaata aggaggcgtt gtattgctta catttttaga tttattcgca
    38401 gggataggtg gctttaggct agggatggaa aaagcaggac ataaatgttt gggacattgc
    38461 gaatatgata aattcgcaaa tttaagttat aatgccatgc acaaaccgaa ggaggatgaa
    38521 tggtttgaaa gagatattag agaaattaga acagaaaata tcccaagagc agatgtctgg
    38581 tgttttggat tcccatgtca agacatttct gttgcaggga aacaatttgg attcagagga
    38641 gaacgttcaa gtttattttt tacagttaca aaacttatta gagaactcaa agaagaagat
    38701 agacccaagt atttacttat tgaaaacgtt aaaaatctac ttagtgttaa tggaggattt
    38761 gatttcctca aagttctcgt tgaactggat gaaatcggtt atgatgcaga gtggcaagtt
    38821 cttaattcta aaaacttcgg agtaccccaa aatagagaac gaatattcat tgttggacat
    38881 tttagaggac gaagtacacg aaaagtattt cctatcgaaa gaaaaagtgg aaaaaatctt
    38941 gagcaactaa ataatccaac tcatagtaca aatagaattt atgatgcagt tggaattgct
    39001 agatgtatta gaagtcaggc aggaggtgga ggtgctaaaa caggtctata ctttatagac
    39061 ttaaataaaa actctaaagt aacaataaat gctagatgcc ttaaagcaaa atataatgca
    39121 ggtgtgacaa atagaaattg tgataatagt ggagttttag ttaatgcagt tttaacgccc
    39181 gatagggtaa ataaaagaca aaatggtcgt agaattaaag aaagcggaga aacaatgttc
    39241 acattgacag ctcaagataa acatggaatt ttgaaaaatg gagatataag aaggttaaca
    39301 ccaaaggaat gctttaggtt gcaaggattt ccggataaat attacgaaag agcagcaagt
    39361 gtatgctcag atagtcaact gtataagcaa gcaggaaatg ctgttactgc aaatgttgta
    39421 tatgaaatag caaaaagaat gggctaaaag ttgcaaaatg tcttttagta tgaatatttt
    39481 tgaagtgttt tgtaactctc aaaaatgaaa ataaggggtg ggataaatgt atgaatatat
    39541 attaagatgg caaataggat tatcgttaga aaatagaaaa atacattata catatggaag
    39601 taaagaagct ttaagaaaga aagcaaaggc attggctaaa gatgaaaata tagtactaat
    39661 aactatagat aaggtagatg aagttataaa aaatactata agcgagaaga ttatagaacg
    39721 ttttgaaaat ttataagggg tggaattatg ataatacaca aatttataat acatgtttta
    39781 gataagaata gtgatacacc aatattgaat gattttgaag gtagggttaa tcaagatatg
    39841 gtcctatttt ttcaaaagaa aataagcaaa gtatcaagag ataatgacat cagaacagca
    39901 gtatttaata actatagtaa caatctaatt aagaagtgtt gtgaacaaat tatttatgat
    39961 gaaagttcat ttttaaataa ctctaaagag attgcagctt atttatttga tgttatgaaa
    40021 ttgaatgcta cattagaatc ttgcgactta gcaatttgtt tatactctca aaaagatgaa
    40081 aagaaagttg ctatattaaa gcttgattac aataattcgt atactcattc aattgagttt
    40141 aaagatgata aatttaatat acagatgtct aaaaatgaaa ttaatataca agagactaag
    40201 acggttaaaa ttgctgcttt ggttggattg agtggaatga atgacaaata tcatcttagg
    40261 gttttagaca aggatgcaga gaaggaagaa gctaattcta agtttgttac agagttctta
    40321 aatgccacta agataaaaga tgataagtat aagactaaga agttcaaaaa tacagctgag
    40381 aattggataa ctaatgctct tagtaatgat ataaaacaag cagaggatgt aagaagtata
    40441 ttaaattata ctttgagaga aaagcatgaa attgatataa atgattttgt tgataaaaca
    40501 attaaagatg ataagttaaa agatagtttt aaagaacata tggaagaaaa aggtcttgtt
    40561 gaaggattta gtatagataa aaaatgggtt gataaaaagc ttaaaaagag aaatataaaa
    40621 actgacaatg gctttgaaat aaaaggtaac ttaactgatt ttgaggaccc aatgaaatat
    40681 acagtaagac aaaatcaaaa tgggtctata gatatagtta ttaagaatgt aacattttat
    40741 gaggaaaagt aggtactcat gtgactattg gctagagaag gagaagtaaa taataagagg
    40801 atgtaaattt aaactagtta ggaggaataa cttatgaaga tttttttatt gactatactg
    40861 ctaataatta tttgtatatt agcaaattat gtgaaaaatc gcatatataa aaaatctata
    40921 aataatctaa aatataaata ttctgtaggg gaaaagatta tatatcatca aataaactgt
    40981 tactataaca gaatggttgg ttgtgaaatt ttagaaaaat gttatagtac gaaatttaga
    41041 aaaagaaata ccccgcttta taaagtaaaa gcatatgtag gtgataacga tacaacatgg
    41101 gttataccag agtggagaat tgaatgtctt gctacgactt atggagaatt tcctaaatat
    41161 taaataataa aaattggctg gagaaggaga ttgtaaatta tgtttaacat ctataaagtg
    41221 aaaataaaga ctaaaagaac attggagcag gtaagaaatc aaagcgtaga ctttgagtat
    41281 tcagaaaaag gattaaaaaa tactctgaaa tactataact tgattgatga tttaaaagta
    41341 atagtagtta aatttggaga tgaatattgt ctagctaatt acaatgaaga agatagaaaa
    41401 ataataatgg aagcacatta tcttttagag caggatgaat atactggatg ttatataaat
    41461 gaatatgaac gatttaaaaa agattgggaa aatggtaatt gtgatgggga agcctgtatg
    41521 gtattttcag atgatgaaat tgagataatt gagaagctaa gggagggtta aatatgaata
    41581 aaagaattaa aatgaaaaaa agattaattc ataaaaagtg tgatgaaaga tgtgtcaact
    41641 atgactttgt aattagcaat aaccttataa cttgtaatgt gtgtatagga tgcaaataca
    41701 aagaaaatat ggataaagta tgtgaagaga actataagaa attaagaagt aaatagaata
    41761 aaatagtcaa ggtaagtttg tgaatgaaac tagaatgtta tagacttact ttgacttata
    41821 aaaggagtgt gttaaatggc taatatatat tgtgaaaatt ataattgtaa aaactacttt
    41881 gaagatatgt gtatgcttga aagaattgaa attaataact tcaaagaatg cgaaagctat
    41941 cttgaaggta aaaatgagct atatgaatta gaaaatggat atactataca tcctaaagat
    42001 ttgaaaatgg tgaaaagtaa agattattct gttgaagtta ctcatattcc aactggtatt
    42061 acagtaaaat gccgttctac aaatagtatt ttaaaaaata aaaataagtg tttggaagtt
    42121 ctagaagaag aactaacaaa aataaactct cacttagagc tagaagattt acgctaaata
    42181 ggaagtgagc ttatgaaacg aagaagatgc agttggtgtg gtaagttatt ttatcttgaa
    42241 gaaaaatcta aggatgttta ttgttgtaaa gaatgtagga agaaggctaa gaaggtgaaa
    42301 aaatgaaagt ttttcttgta atagatggag agccagttgg caaggaaaga ccaagaatga
    42361 atacttacgc taaaaggacc tatacaccta ataagactaa gaattatgag gacctaataa
    42421 aatggctata tcaatctaaa gttaaatatt actttgaagg ttatataaaa atgactttaa
    42481 gatgttatta ttctatagct aaaagtaaca gtaaaaaggt taaggagcag aaaagaaata
    42541 atgtgttaag acctagtaaa aagcctgaca ttgataatgt cattaaagta gtagctgatt
    42601 cactcaatga gatagcttat aaggatgata cacagattgt tgaggttgta gctagtaaat
    42661 attatagcga taatcccagg gttgaggtta tattagaaga tgttatctaa ccaacggaaa
    42721 aatccgttcg ttaaattatg cccttagttt ttcataaaaa tgtgaaaaag ctaaataaag
    42781 aatatatcaa acgacaaagg agagataaat tatgaatgaa aatataaata aagaaataac
    42841 agtacttgga actttagaaa tcgagggaat gaaatttcat aacattgagg gtggatttgg
    42901 agaacataag aaagcaatgc tagtaaaaga tatagccgag atacataata gagaatctag
    42961 acagattaat gagcttataa ataagaatag aaaaagattt aaagatggaa aagatatatt
    43021 agatttgtta ggtgtcggtt tggacgatac caaaataaaa gaattaggat ttactcaaca
    43081 atcaattaat tcttatagag ggttaaaaaa caaagggtta ttatctggga tttatatatt
    43141 atctgaaaga ggttatgcaa aattattaaa aatattagaa gatgatatag cttgggaatt
    43201 atatgagaag ttagttgatg gatatttctc tatgagaaaa gaactaaata atcctctttt
    43261 aagtgcatca aaggagttac aggctatatt tatgctagat aagaaacaag aagtcttaga
    43321 aactaaaata gagaatgtta atgagaaatt agagaacttt atggatgatg caccactatt
    43381 caatattgaa tgtgaaagta ttgtaaaaga agttaagaag gtagcaacaa aatcactagg
    43441 aggtcatgga agtaaggctt ataaaaataa atctttaaga ggtaaagtat acaatgatat
    43501 ataccatcag attaaacgag agtttggagt agatagttat aaggctataa agcgttgtca
    43561 attaaataaa gtattagaga ttgtaaacaa ttataagtta cctatagtgt ttgaagaaga
    43621 aataagactt ttaaatagtc aattatcaat agtaagttaa atttattcaa aaaaaaggaa
    43681 aaggagtgct ttcacactcc acttgtcaaa aatataaaac ttttatccaa gattattata
    43741 acataaacag gagtgtggaa gtatggataa taatatcaat aaaaaagaac tatttaaaaa
    43801 agtagaaggt agattacatc attataaatt tttaagtgca gaaattaaga atcttgaatt
    43861 agatatagaa agtagagaaa atgagatatt tgggtgtaag gctgttggat atgatgaaaa
    43921 agtaagtcca acatatgctt ttaattcaac tgttgagaat gagattataa aaaaagaaag
    43981 agatattact agattgaaaa aactgaaaaa agataaagaa atagaaaaga agaaaataga
    44041 aaatgcactt acatgcttag atataagaga agaacatttt tttaaactgt tttataatag
    44101 tagaatgaaa aatagtatgg tttatatatc cttagagatg aactcagata ggaaaacatg
    44161 tagatgtgta agggaaagat tagtgtataa agttatggat atgctttatc caagaattaa
    44221 ggaaaatgaa ctcccattat ttaaaaatta gaaaattccc cagtttttcc ccagaaattc
    44281 ccactttatt ccctactttc tccccttttt gattaaaaaa gcatgagata atagtattgt
    44341 ggaaataaag atttccctct caaaacttaa tatttgacta gggtataagg gattgcccta
    44401 gtcactacga acagactagg cagggcgtga ggacgctgtt agttcaattc taactatgtt
    44461 caaatattaa tcaacgtata cactaaaagt agagaaattg agggcaaaat tttatatttt
    44521 gtatcttaat tcagaagtct aaaaatcggg tggggcttgg taacctcact caccatgcag
    44581 gtactggtgt ctagtctaag ttcgattctt agaacctgcg acataatata tgtatctccc
    44641 tactaaaaat gctaagttta ctccaaactt agcattttaa tttttaaaaa gaaaaaagaa
    44701 atttttattg tcataatact atttgtttag gtatattata atgtgcatac ttaaaattaa
    44761 atacttagca agcatttgaa ttaatataca taagatatat gacataattt tttatagtgt
    44821 aagttattta aattaattat aaataacagt aattttatta tataaaatgt acatattgtg
    44881 aataataata ataaaatcat gtacaaaatg ccaactgata attcctcgaa atatattgca
    44941 tattt
//
LOCUS       DAGQWR010000011        44432 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.011, whole genome shotgun sequence.
ACCESSION   DAGQWR010000011 DAGQWR010000000
VERSION     DAGQWR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44432
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.011"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            393..1589
                     /locus_tag="MIW95_000532"
     CDS             393..1589
                     /locus_tag="MIW95_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBY3149885.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1682..2206
                     /locus_tag="MIW95_000533"
     CDS             1682..2206
                     /locus_tag="MIW95_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBY3149886.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            2231..2407
                     /gene="rpmF"
                     /locus_tag="MIW95_000534"
     CDS             2231..2407
                     /gene="rpmF"
                     /locus_tag="MIW95_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBY3149887.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2614..3171
                     /gene="fapR"
                     /locus_tag="MIW95_000535"
     CDS             2614..3171
                     /gene="fapR"
                     /locus_tag="MIW95_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBY3149888.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            3182..4204
                     /gene="plsX"
                     /locus_tag="MIW95_000536"
     CDS             3182..4204
                     /gene="plsX"
                     /locus_tag="MIW95_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBY3149889.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            4230..5216
                     /locus_tag="MIW95_000537"
     CDS             4230..5216
                     /locus_tag="MIW95_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBY3149890.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5325..6254
                     /gene="fabK"
                     /locus_tag="MIW95_000538"
     CDS             5325..6254
                     /gene="fabK"
                     /locus_tag="MIW95_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBY3149891.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            6270..7220
                     /gene="fabD"
                     /locus_tag="MIW95_000539"
     CDS             6270..7220
                     /gene="fabD"
                     /locus_tag="MIW95_000539"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBY3149892.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIEILLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            7220..7969
                     /gene="fabG"
                     /locus_tag="MIW95_000540"
     CDS             7220..7969
                     /gene="fabG"
                     /locus_tag="MIW95_000540"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBY3149893.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTRVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            8023..8247
                     /gene="acpP"
                     /locus_tag="MIW95_000541"
     CDS             8023..8247
                     /gene="acpP"
                     /locus_tag="MIW95_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBY3149894.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            8276..9514
                     /gene="fabF"
                     /locus_tag="MIW95_000542"
     CDS             8276..9514
                     /gene="fabF"
                     /locus_tag="MIW95_000542"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBY3149895.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9740..11458
                     /locus_tag="MIW95_000543"
     CDS             9740..11458
                     /locus_tag="MIW95_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY3149896.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLNIGSYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMKSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDNIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11489..13489
                     /locus_tag="MIW95_000544"
     CDS             11489..13489
                     /locus_tag="MIW95_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3149897.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKEKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNVRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTKKEFEEILRILYERRESSSGVGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13837..14082
                     /locus_tag="MIW95_000545"
     CDS             13837..14082
                     /locus_tag="MIW95_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBY3149898.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            14117..14842
                     /locus_tag="MIW95_000546"
     CDS             14117..14842
                     /locus_tag="MIW95_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBY3149899.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEEENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14864..16177
                     /locus_tag="MIW95_000547"
     CDS             14864..16177
                     /locus_tag="MIW95_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY3149900.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVCGENLKKIV"
     gene            16476..16922
                     /locus_tag="MIW95_000548"
     CDS             16476..16922
                     /locus_tag="MIW95_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3149901.1"
                     /translation="MGGETHFLSFGKDEYKISLEEQENAIESAKASDNSVKLIAFIDD
                     EIVGIATIDSNQKAKGKHVGVLGIVVKEKYWGIGLGKRLMLDLIEWCKSNGITKKITL
                     VTNEENYNAIGLYKKVGFEVESILKKECYYNGVYTDLIGMSLLLGI"
     gene            17011..18996
                     /locus_tag="MIW95_000549"
     CDS             17011..18996
                     /locus_tag="MIW95_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBY3149902.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTITLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIEEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            19220..20188
                     /gene="spoIIIAA"
                     /locus_tag="MIW95_000550"
     CDS             19220..20188
                     /gene="spoIIIAA"
                     /locus_tag="MIW95_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBY3149903.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIDD
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            20178..20699
                     /locus_tag="MIW95_000551"
     CDS             20178..20699
                     /locus_tag="MIW95_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBY3149904.1"
                     /translation="MQIKIIIIALLVGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20716..20913
                     /gene="spoIIIAC"
                     /locus_tag="MIW95_000552"
     CDS             20716..20913
                     /gene="spoIIIAC"
                     /locus_tag="MIW95_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBY3149905.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20929..21315
                     /gene="spoIIIAD"
                     /locus_tag="MIW95_000553"
     CDS             20929..21315
                     /gene="spoIIIAD"
                     /locus_tag="MIW95_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBY3149906.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21400..22605
                     /gene="spoIIIAE"
                     /locus_tag="MIW95_000554"
     CDS             21400..22605
                     /gene="spoIIIAE"
                     /locus_tag="MIW95_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBY3149907.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22665..23282
                     /locus_tag="MIW95_000555"
     CDS             22665..23282
                     /locus_tag="MIW95_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBY3149908.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            23294..23848
                     /locus_tag="MIW95_000556"
     CDS             23294..23848
                     /locus_tag="MIW95_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBY3149909.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKKQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23875..24564
                     /locus_tag="MIW95_000557"
     CDS             23875..24564
                     /locus_tag="MIW95_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBY3149910.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSDSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24674..25036
                     /locus_tag="MIW95_000558"
     CDS             24674..25036
                     /locus_tag="MIW95_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBY3149911.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            25121..25627
                     /gene="nusB"
                     /locus_tag="MIW95_000559"
     CDS             25121..25627
                     /gene="nusB"
                     /locus_tag="MIW95_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBY3149912.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25611..26681
                     /locus_tag="MIW95_000560"
     CDS             25611..26681
                     /locus_tag="MIW95_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBY3149913.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26678..27883
                     /locus_tag="MIW95_000561"
     CDS             26678..27883
                     /locus_tag="MIW95_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBY3149914.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVMVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27885..28100
                     /gene="xseB"
                     /locus_tag="MIW95_000562"
     CDS             27885..28100
                     /gene="xseB"
                     /locus_tag="MIW95_000562"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBY3149915.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            28103..28990
                     /locus_tag="MIW95_000563"
     CDS             28103..28990
                     /locus_tag="MIW95_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBY3149916.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            29020..29457
                     /locus_tag="MIW95_000564"
     CDS             29020..29457
                     /locus_tag="MIW95_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBY3149917.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29491..31356
                     /locus_tag="MIW95_000565"
     CDS             29491..31356
                     /locus_tag="MIW95_000565"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBY3149918.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANNNED
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31489..32301
                     /locus_tag="MIW95_000566"
     CDS             31489..32301
                     /locus_tag="MIW95_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBY3149919.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEYTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32321..34000
                     /gene="recN"
                     /locus_tag="MIW95_000567"
     CDS             32321..34000
                     /gene="recN"
                     /locus_tag="MIW95_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBY3149920.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLKSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGSFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            34215..35081
                     /locus_tag="MIW95_000568"
     CDS             34215..35081
                     /locus_tag="MIW95_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149921.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECTIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGDISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAQLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(35172..35663)
                     /locus_tag="MIW95_000569"
     CDS             complement(35172..35663)
                     /locus_tag="MIW95_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3149922.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIVEAKKIGYTHMRLDTLPSMKSAQGLYEKFGFYDIELYTYNPIEGARYME
                     LKL"
     gene            complement(35724..36164)
                     /locus_tag="MIW95_000570"
     CDS             complement(35724..36164)
                     /locus_tag="MIW95_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3149923.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGCGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFKFNGELDYGGEKVMVLKSN"
     gene            36325..37383
                     /locus_tag="MIW95_000571"
     CDS             36325..37383
                     /locus_tag="MIW95_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBY3149924.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVGYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37525..38349
                     /gene="spo0A"
                     /locus_tag="MIW95_000572"
     CDS             37525..38349
                     /gene="spo0A"
                     /locus_tag="MIW95_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBY3149925.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38762..39883
                     /locus_tag="MIW95_000573"
     CDS             38762..39883
                     /locus_tag="MIW95_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149926.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39901..40776
                     /locus_tag="MIW95_000574"
     CDS             39901..40776
                     /locus_tag="MIW95_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBY3149927.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIVLKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNSLGNTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40800..41492
                     /locus_tag="MIW95_000575"
     CDS             40800..41492
                     /locus_tag="MIW95_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY3149928.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41500..42324
                     /locus_tag="MIW95_000576"
     CDS             41500..42324
                     /locus_tag="MIW95_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBY3149929.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42329..43396
                     /locus_tag="MIW95_000577"
     CDS             42329..43396
                     /locus_tag="MIW95_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBY3149930.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGKIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43398..43934
                     /locus_tag="MIW95_000578"
     CDS             43398..43934
                     /locus_tag="MIW95_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBY3149931.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            44002..>44432
                     /locus_tag="MIW95_000579"
     CDS             44002..>44432
                     /locus_tag="MIW95_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149932.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALI"
BASE COUNT        18017 a         4470 c         8288 g        13657 t
ORIGIN      
        1 ttttaattat aaaattaaaa aacgtaaaaa aatttataaa aatgtagaaa tgttacctac
       61 ataaaaaact ggttaaatgt tattaaaata actttattag ctctttatat tatattatta
      121 taacaaatgg gaaagaataa tctacattaa gttaacttgt taatatatta ttagtgaaat
      181 acacaattta ccatactaga aatatgatat aatataaaaa aaaagtatat acttttatat
      241 taaaaggtga tatattattt atgaaaatcg tttaatgtat acaataatat atctataaca
      301 aaataataaa agatagagtt ttatttagac atttattaca aatcaaaatt tattacatat
      361 tatttataaa aaaatctaag gaggaaataa taatgaaaat attagtatta aactgtggga
      421 gttcttcatt aaaatatcaa ttaatagata tgaataatga agaagtttta tgtataggat
      481 tggttgaaag aataggaata gaaggttcta tattaaaaca tgaaaaagca ggtagagatg
      541 ataaatacgt tgttgaacaa ccaatgaagg accacaaaga tgcaatagca ttagttttag
      601 aagctgttgc tcatccagaa tttggtgcag ttaaagaaat gaaagaaata gatgcagtag
      661 gacatagagt tgtacatgct ggagaaaaat ttgcaacttc agtagtaata actccagaag
      721 tagaagaagc tttaaaagaa tgtatagatt tagctccact tcataatcca gcaaacataa
      781 tgggaataga tgcttgtaaa gctatacttc cagatgtacc aatggtaggt gtatttgata
      841 ctgctttcca tcaaacaatg cctaaatctt catacttata tggtttacct catgagctat
      901 atactaaata tggggtaaga agatatggat tccacggaac ttctcataat tatgtatctc
      961 agagagcagc agagatatta ggaaaagata taaaagattt aaaaatagta acttgtcact
     1021 taggaaatgg tgcttctata gctgctgttg atggtggaaa atgtgtagat acatctatgg
     1081 gattcactcc attagaagga ttaataatgg gaactagatg tggagatata gacccagcta
     1141 tattaccatt cttaatgaga aaagaaggtt tagatgctga tggattggat aaattaatga
     1201 ataaagaatc tggagtatat ggaatgactg gaatttctag tgacttcaga gatatagaag
     1261 atgcagctaa aaatggagac gaaagagctc aggctacatt agaagcgtat gttaaaaaag
     1321 tacaaaaata tataggcgct tatgctgctg aaatgaatgg attggatgtt gtagtattta
     1381 ctgcaggcgt tggtgaaaat ggaaaggcta taagagcaga tattgcttct aacatggagt
     1441 ttttaggaat gaaattagat aaagaagcta atgatgttag aggaaaagaa acagtaatat
     1501 ctactgctga ttctaaagta aaaatgcttt taataccaac taatgaagaa ttaatgatag
     1561 ctagagatac attaagattg gtaaaataat taaattaaac ctatgcctat caagtaaaaa
     1621 tacttgacaa acaaatgcta gatttgtata ataaatttgg tgatatgttg aggtgaactt
     1681 aatgaaagtt agcatagaaa aaataaatag aaaagaaact gacaaaatag acttgaattt
     1741 ttgtgaaaaa attgatacta ttagttattg tgatgagatt tacaaattag tatcacctgt
     1801 aaatttaaaa gggaaagtgt caaagactaa taaaggtttg tatctagata tagatgtcaa
     1861 ttttacaatt gttgacaatt gttcaagatg tcttaaagaa gtagaaatac cactagagta
     1921 ttctatacaa ggttttttag taaaagaaga agattatgat gaagatgagt ttgaggaatt
     1981 tgatcctttt atattcgatg gtgaagaaat cgaccttatt gatataatag aacagacatt
     2041 agattttaat gtacctcata aagttctttg tagtgaaaac tgtaaaggtc tttgtcaagt
     2101 atgcggggca aacttaaatg aagaagagtg ttcttgcagt gaaattacaa acgatgagga
     2161 atacatagat cctcgttttg ctaaattaaa agatttattt aattaaattt tgactaagga
     2221 ggtggcgtaa atggcagtac caaagcgtaa aacgtctaaa tcaaacacaa aaatgagaag
     2281 agcagctaac tcaaagatgg aagcaacagg atttgtaagt tgcccacaat gtcatgagcc
     2341 aaaattacca catagagtgt gtccagactg tggatattat aaaggtaaag aagttgtgtc
     2401 taagtaatag cttctaaaaa gcatgtagag tatactctac atgttttttt tgtacatata
     2461 aaaaaataag atgatttact agagaaaata agtggatttt atgcatatta aaaaatagaa
     2521 aaataataat ataagtattg aaaatttttg tgtgttacta tatactacta ttagtagctg
     2581 gtactaatta cagctataaa atttggaggg aacatgaaaa agaaaagtaa agcccaaaga
     2641 caaaaagagc ttatagatat gctaaagaca gatccttttt atactgatga agaattatca
     2701 agtctgtttg atgtaagtat acagaccata agattagata gaatgagtct aaacatacca
     2761 gagctaagag aaagagtcaa atcaatagca gaaactcaga gttcaaaagt aaagacttta
     2821 ggagtaaagg aaattacagg agaaatcatt gatttatctg tagggcgatt aggtatatct
     2881 atgttagaag ttacacagga catgatttac tctaagacta atactttaaa agatacgtat
     2941 attttttctc tagcagattc attggcaatg gctataatag atgcacctaa ggttattatg
     3001 agagttgcaa atgtaaaaag ttttaagttg atagaacaac aagatagact tatagcaaaa
     3061 gcagaagtgt atagaaatat agataaaaag cattatgtaa aagttgttat caacaataaa
     3121 gcccaagagc aaatatttag aggaaagttt atctttgaag aattagatta ggagggaaat
     3181 tatgaaaata gtaattgatg gaatgggtgg agataatgca cctaaatcta atgtagaagg
     3241 cgcagtaaat gctataaaag aatatcaggt tgatttaata atcacaggag ataaagattt
     3301 acttgaaaag gaattttcaa attatgaatt tgatagaaat aaattagaga tagttcatac
     3361 tactgagatt atagaaaacg aagataaacc agtaaaggct ataagaagta aaaaagattc
     3421 atctatggta gtagcattaa atcttgtaaa agaaggaaag gcagatgcta taatatcagc
     3481 tggaaataca ggagccttac ttgctggtgg attatttgtt gttggtagaa taaaaggaat
     3541 cgatagacct tgcttatgtt cagcaatacc aaatgtaaag cgaggaatga cacttatagc
     3601 agactgtggc gcaaatgcag attgtaaacc taagaatcta gttgaatttg cagctatgag
     3661 taatatttat tcaagaaaag ttttaggtct tgaaaatcct aaggtagcat tagcaaatgt
     3721 tggactagaa gaaggcaaag gcaatgattt agtcaaaagg tcatatgaag aaattaaaaa
     3781 attagattta aattttatag ggaatgtaga agctagagaa gttataaatg catacacaga
     3841 tataatcata tgtgatggat ttacaggtaa tatattactt aaatctgctg aaggtgtagc
     3901 actttcagtt atgagtctta taaaggaaac ttttatggcc agtacaaaaa gtaaaatagg
     3961 agcattactt ataaaggatg atttaagaaa attaaaaagt tttatagatt attctgagta
     4021 tgggggagct ccacttttag gtctaaatgg aggagttata aaagctcatg gtagttctga
     4081 tgctaaagct ataaaaaatg ctataaatca aggaataaaa ttttcaaagg gaaaagtcgt
     4141 tgaagatatt aaccaattta taagcaaata caatgaagaa aataaaaata acgaagatga
     4201 ataaactcgt tttggaggtt taatatataa tgaatacaaa ggctggaata ttaggagtag
     4261 gaagttatct acctgagcaa tcatatgata attttcactt cgaaaaaata atggatacat
     4321 cagatgagtg gataagtact agaactggta ttaaggaaag aagatttgcc aaagagtctg
     4381 aagcaacttc tgacttagca tctaaagctg ccttaaaagc tatagaatgt gcaaagttaa
     4441 acgtagagga tatagagctg attatattag ctacaattac tcctgatatg tctttgcctt
     4501 caactgcatg tatagtacaa gatgcaatag gagcagtgaa tgctactgca tttgatatat
     4561 cagcagcttg ttctggattt gtatatggag taactattgc aaagcaattt gtggaaacag
     4621 gttgctataa aaatgtgctt gtaataggag cagaaacttg ttctaagttt ttaaattatg
     4681 atgatagaac aacagcagta ttgtttggag atggagctgg agcagctgtt attggtcctg
     4741 taaatgaggg tggaatactg tctacacata tgggctctga tggtaaagga aaagattgtt
     4801 taaaagtacc tgctggaggt tcaagactta aggcaagtaa agagactgta gaagctaatc
     4861 tacatacaat agaaatggca ggtagtgatg tgtttaaatt tgcagttaga aagatggcag
     4921 aaacttcttt aagagctctt gaaaaagcaa acctaaatac aactgatata gactacttag
     4981 tgccacatca agctaatata agaatcatac aagcttcttc taagagatta gagcttgata
     5041 tgaaaaaagt ttatgtaaat atagacaaat atggaaatat gtctgctgca tctataccag
     5101 tagcattaga tgaagcttat agagaaggta aaattaaaaa gggtgataat gtagttttag
     5161 ttggctttgg tggaggtcta acttgggggg cttcagttgt caagtggact ttatagtata
     5221 aaatatctaa aatgatttaa ttaacaaaat taaacttata cacaaaataa agttgattaa
     5281 taataaaaag tttactttta tgatttcaag tgaaggagaa ttgtatgaat aaaatttgca
     5341 aaatattaaa tataaaatac ccagttatcc aagggggaat ggcatgggta gctactgcat
     5401 cattagcaag tgctgtatct aatgcaggag gacttggcat aatagcagca ggaaacgcac
     5461 caaaagaagc tataaagaaa gaaattgttg agtgtaaaaa attaacagat aaaccttttg
     5521 gagtaaatgt aatgcttatg tcgccatttg ttgatgatat aattgatttg attatagaag
     5581 aaaaagttca agttattact actggtgctg gaaatcctgc aaaatatatg gatagattaa
     5641 aggaagctgg aacaaaggtt attcctgtag tacctacaat agctttggca caaagaatgg
     5701 aaaagttagg agctacagca gtaatagcag aaggtactga aggtggagga catataggag
     5761 aacttactac tatggtctta gttccacaag ttgctgatgc tgtaaacata cctgtaatag
     5821 ctgctggagg aattgtagat ggtagaggaa ttgcagcatc atttgcatta ggtgccagtg
     5881 cagttcaagt aggaactaga tttatttgca gtgaagagtg ttctgtgcat tcaaactata
     5941 aaaacttagt actaaaagca aaagatagag atgcaattgt aacaggaaga agtactggtc
     6001 atccagtaag aacattaaaa aataaactat caaaagaatt tttaaagatg gaacaaaatg
     6061 gagctactcc tgaagaattg gataaaaaag gtacaggagc tttaagattt gcaacagtag
     6121 atggagacat agaaaaaggt tcatttatgg caggtcaaag tgctgctatg gtaaaagaaa
     6181 taacaccttg taaggaaatt atagaggcta tggtaaatca agcaagagag attatgccag
     6241 caatagaact gtaaatcagg aggagcaaga tgggaaaagt tgcactagta ttccctggtc
     6301 aaggagctca atatgtaggt atggccaagg atttatatga aaataatgct gttgctaaaa
     6361 gtgtaataga tgaggctagt gatgccttaa atatggattt aaaaaacctt atgtttaatg
     6421 gaaatgaaga agaactttct aaaactgaaa acactcaacc tgctatggtt acacatagtg
     6481 tagcagtatt aaaagcggta caagctcaaa tagatttaaa atataatgca tgcttaggat
     6541 tatccttagg agagtatagt gcgttggttg cagcagatgc aattgacttt aaagatgctg
     6601 tatgtttagt taaaaaaaga ggtaagttta tgcaggaaac agttccagca ggtgttggag
     6661 ctatggcagc aattttaggt ttagatagaa atgtacttga aaatgtggtt aaagatatac
     6721 aaggaggtat tgtagaagta gcaaattaca attctcctgg acaaatagtt atatctggtg
     6781 aaaatgaaaa gatagaagaa gcaatggtaa aatgcaaaga agcaggagca aaaagggcag
     6841 ttaaattaaa tgtaagtgga ccttttcact catcaatgtt aaaagaagct ggtgtaaaat
     6901 tggccagtga attagaaaaa gttaacataa ctaaacctaa agttgatgtg gtggcaaatg
     6961 taaatgcaga ttactataaa aacgaaggta aagacctgct tataaaacaa gtaagttcat
     7021 ctgttctttg ggaagattct atagaaatac ttttaaatga tggatatgat acttttatag
     7081 aaatgggacc tggaaaaact ttaaaagcat ttattaagaa gatagcttcc aataaaaaag
     7141 caaatgtcaa tatttataat atagatggta tagattctct taatgaattt gtacaaaatt
     7201 atagaaatgg agagatataa tgataaatct tacaggacaa gtagcagttg ttactggtgg
     7261 ctctaggggt ataggaaaag aaatagcaaa aaaactagca tcttttggag ctgatgtagt
     7321 aatcaattat acttctaaag aagatgaagc actaaaaact aaaaatgaaa tagaaagtat
     7381 gggggtaaag tgtacctcta taaaatgtga tgtgtctaaa tttgatgaag tgaatcaaat
     7441 gatagattct gttgtaagcg aatttggaaa aattgatata ttggttaata atgcaggcat
     7501 aactaaagat ggtctgctta tgagaatgaa agaagaagat tttgatagag ttatagatat
     7561 aaacttaaaa ggtgtcttta attgtacaaa agcagttact aaacctatga tgaaaaagaa
     7621 gtatggaaga ataataaata tgacttcagt agttggaatt atgggtaatg cagggcaaac
     7681 taattattgt gcatcaaaag caggtgtaat tggatttaca aaagcttctg caagagagtt
     7741 agcatcaaga aacataaata taaatgcagt agcacctgga tttatagaaa cagatatgac
     7801 tagagtacta agtgatgatg taaaagaatc aacactagca aacataccaa agaaatctta
     7861 tggtaaacca gaagatgtag ccaatgccgt agcattttta gttagtgaca tgtcaagtta
     7921 tataacagga caagtaataa atgtagatgg tggaatggta atgcaataat aatttaaaaa
     7981 gtagagaaat ctattaaaat aaaaaatttt aggaggtaca caatgtttaa taaagttgta
     8041 gaaataataa tagagcaatt aggagtagaa gataaagaaa taactatgga gacatcatta
     8101 atgaaggatt tagaagcaga ttcattagat gcagtagaaa taataatggc acttgaagat
     8161 gaatttggta tagaaatacc agatacagaa gcagaaaact tcaaatctat aggagatata
     8221 gttaagtata tagaagctaa taaatagtcg agctttaaaa aagtggaggt atccgatgaa
     8281 taaaagagta gtaataacag gacttggatg tgttacgccg ttgggaacgg gaaaagaaga
     8341 gttctggagt aatataaaat ctggagtatc tggtatagat aaaataacta attttgatgc
     8401 tagcacatat caaactcaga tagcaggaga agttaagaat tttcatccag aagaatatat
     8461 aagtaaaaaa gaattgaaga gattagataa atttgctcaa tttgcaatag tatctgctaa
     8521 attagcagtt gaagatgcaa atcttgattt agataaagtt gatagagaac gttttggtgt
     8581 tataataggt tctggaatag gtggagttga agctatagaa actcaacata aaattctttt
     8641 ggaaaaagga aataaaagag taagttcact ttttgtacct atgatgatag gaaatatggc
     8701 agctggtcaa gtttcaatat ttttaggagc aaaagggcct aacactaatg tatgtacagc
     8761 ttgtgcatca ggaactcatt caataggtga tgcttttaag gtaattcaaa gaggtgatgc
     8821 agacatcatg gtagcagggg gctctgaagc tgctgtaaca ggtcttgctt ttgctggatt
     8881 ctgtaacatg aaggctatgt caactagaaa tgatgaccct aaaactgcat caagaccgtt
     8941 tgataaagac agagatggtt ttgtaatggg agaaggagca ggtattgtta tcttagaaga
     9001 cttagaacat gctttagcaa gaggggctaa aatatatgca gaagtagttg gatatggttt
     9061 aactgctgat gcataccata tgacaactcc agctgaaaat ggagagggag cagctaggtc
     9121 tatgaatatg gctttgaaag atggaaatgt tccattagaa gaagtagatt atataaatgc
     9181 tcatggaact tcaacttatt acaatgattt atatgaaact atggctataa aaactgtatt
     9241 tggagaaaag gcatatgact tatgtgtgtc atcaacaaaa tctatgactg gtcacttatt
     9301 aggagcatca ggtgctatag aggctgttgt ttgtgctatg agtatagaag atagttttgt
     9361 gcctccaact ataaacatac aagaagttgg tgaagatttg gatttagatt atgttccaaa
     9421 tcaaggtaaa gaaaaaaata ttagatatgc actatcaaat tctttaggat ttggtgggca
     9481 taatgctaca atagtactaa aaaaatatgt ataaataaat attaaatata aaactaaaaa
     9541 acaagcactt gttttaactg tataaattta tattagctta aatttaaagt ctcctaaatt
     9601 attttaggag acttttttat taagatttaa gtctagcata tgttgtaaat cattttgata
     9661 tattgtatta ttaattatta agaggttatt tatatatgca agttttcatt ctacaatata
     9721 caaaaggagg actaaaattt tgaattttag actaccaaag aaaaggttac ccaaaaagat
     9781 aatttttata tgtataatac ttattagtat tattttgttt ataaagtatg accaaaatgg
     9841 attgaaagaa agagaaatta taaataaggg aaaatatatg tctgaaagcc ttgtggcaga
     9901 taaggagtat gatgaagcaa aaaaagtggt taagacttct ttcgaatcta ttccagaaaa
     9961 aaaatacaat aaagtatata atgaagtttg ggatatgtta aagactattt catatgttcc
    10021 agatggaacg aagatagtca ttgattcttt agaaaagatg agagaatcag atgtgaaatt
    10081 atctgatgaa tgtagattta atatagaaaa aagattagca tcagtgtata taatggataa
    10141 taattattct aaggcagtag atttaactgt aaagtctata aaattggctg agaaattggg
    10201 gaataattat gaaaaagcaa gactagatgt tgatttggcg agcatatttt taaatatagg
    10261 tagctatgaa acaggagaga aattaataaa ggattctttt aaaatagata ttgatgaagg
    10321 tgaaaaaaat ggaatggtta gattatatgc tattataaat ctagcagaaa tatatgtaga
    10381 agagggagaa tatgataaag ctattgaaat aagtagcagg ataaaagatt ataaaaagtt
    10441 tgtaaatacg gataattaca atgattttga tataatggcc tctataatga agtcaaatgc
    10501 gtatgtgggc aaaaataata tacaaaaggc aaagactttt ttagaaaaag ctgattatct
    10561 tataaaatca gatagaacag tttatatatt agataaagat atgtactatt atatggcaat
    10621 gggaaactta gaatataaaa gcgaaaatta taattctgca atagataatt ataaaaaaag
    10681 tttagagata tctaccaaaa gaaagttaac tcaggagaaa ataaaaaatt taaataagat
    10741 attaaatata tatgaaaaac aaggaaatat agaatcttta aatgagtatc aaaagatgtt
    10801 aataaatgaa tataaagaga acaaaagtat aagagattca gagactagtt tctatataat
    10861 tgataaagta tcaaacgaaa gtaagctatt tgaaaaaact aaaaaagaaa taaaatatta
    10921 taaaatatta tttacagtaa ttttaattgc tatattaggg tctacttttt tatacaaccg
    10981 attgaagtat tttaaagcac aaaatttaca tgatggtctt acaaatgttt ataatagaaa
    11041 aagttttgat attatgtatg aaaagtacag agagaaagat gataattttg cacttgtgat
    11101 gatagatatt gataatttca agctcataaa tgataattat ggacataaat ttggtgacac
    11161 tgttataaag ggaataacaa ataccatttg tgttatgtta gaacaagatg ataaagtctt
    11221 taggtacgga ggagaagaat tttctgtatt aataagaaat aaatctggtg aggaagtagc
    11281 agatataatt gacaatataa gggttgcaat tgcaaataaa aagtggaatg atgatgtgac
    11341 tgtgactata agtgcaggtg tagctcatat ttcagatagc aaagatgtac tagaagaagc
    11401 tgataaaaat ttatataaag caaaacaact tggtagaaat aaagtagtat ataagtaatt
    11461 atatactata taaataagag gtataaatat gaaacaatca atagctatag taactgataa
    11521 aataactaaa ctatcatctt tcttagaaga aaatatacgt ttggttttag gtaattatat
    11581 tgatataaac cactattatc tggaagtttt aaaagagaat gataaaatac aaggtgattt
    11641 tgtactagta atgaatgatg atagattaaa cagtatgaga aagcatttag tacaagatag
    11701 taagattata gtgataagga gaacacttaa agaaaatgaa atatatggat tattttcaat
    11761 accaaatgga actgaagtat tggttgtaaa tgatacaaaa gaaactacct tagaaacgat
    11821 aagcttattt tacaaaatag gagtaaaaaa tttaaagttt actccatata ttgaaggaaa
    11881 aatttataaa aatatagaca tagctataac gcctggagta aaggaaaaag taccaagtta
    11941 tataaataaa gtaatcgacc ttggtaatag atatattgat atatctactt ttatagaaat
    12001 aataaatggt cttagaattg atgttaaaga aatacgtaaa aatttaatgg catattctga
    12061 gagtttaata agtttagata atggagttaa agataattat aggcaactgt ttttaaaaat
    12121 agaagaacaa gatgttatat tgaatctatc aaaagatgga ataatattta cttcagcaga
    12181 tggtataata aacacattta ataaagaagc tttaaaaatt ttaggtataa atgaaaatcc
    12241 acaaggcaaa aacatagaag atatcatttc tgaagattta aatattttac ttgaagacaa
    12301 agaaataatt gatgaagtgg tatttacaaa taaaaaatat ataaatgtaa ataaaaaaag
    12361 tattttttct atggggaata aagctgggat atattataac ttacaagaaa taacgtatat
    12421 aaaaaaatta gaacaaaatc ttactaataa attaagagaa caaggtcaga tagcaagata
    12481 tacatttaaa gatataaaaa ctaaaaataa aaatatggta aagtgtgtag aattggctaa
    12541 aaaaatatct aaatcagacc tttctgtatt aataatagga gaaagtggta ctggtaaaga
    12601 attattatca caatcaattc ataatgcttc caatagaagt aatcaaccat ttatagcggt
    12661 taattgtgca gcagttccag atagtctatt agaaagtcaa ctgtttggat atgaaaaagg
    12721 ctcttttaca ggagctttaa gagaaggaaa aaagggtcta tttgaacttg ctaataatgg
    12781 aactatattt ctggatgaaa ttggagatat gcctccattg ctacagacaa agttactaag
    12841 ggtattacaa gaaaagcagg ttatgcctat aggttcccat aatgttataa acatagatgt
    12901 tagggtcata gcagcaacaa acaaaaactt actaaagatg attagagaag gaaaatttag
    12961 agaggacctg tattatagac taaatgtatt gccaatagat gtaccatcat tgaggaatag
    13021 aaaagaagat ataataacac ttatgaattt ctttttgaaa aacaatgtaa gactttcgcc
    13081 agaagttata actattttag aaaaatataa ttggccagga aatataagag agttacaaaa
    13141 tgttgcttct tatgtaagtc taatgtgtga tagtatggtt tttagagatg acttaccacc
    13201 atatataagg gtagaatgtg ctaacaatta tgaagatgga tttggttata caaaaaaaga
    13261 atttgaggaa atactgagga ttctatatga aagaagagag tcatcctctg gagttggaag
    13321 agggtttata atcgaaaagc ttatacagga agggattgag atttctgaaa gcaaagttag
    13381 aaaggtgcta tcttatttaa gtgaatgtgg atatatatca tctaaatctg gtagaggagg
    13441 aagtcaaata acctcagagg ggataaaact ttataataat ataaattaag ctaagtattt
    13501 gatattgaaa tcacatattt tgaatttaga tatttaaata ataaactttt atatttatag
    13561 agattaattt atactatgtc agaaagaata ttgtaattta taattaaata ttaataggtt
    13621 tttaaaatag gattagatag gtaataggtt aagttagaat aacccattac ctatttttat
    13681 ttgtaagaaa agtgtacata gagttaaaag taaaaaatac aactaataaa actattgaaa
    13741 acactagata aataataaat aaaaataatt ataaattttg gcatatatat tgctaatcat
    13801 agagataaga cgacaaaata attgggaggt ttaatgatga aatacaaaac aacagggact
    13861 tgtgctacag aaattgaatt tgaagtaaaa gaaaataaag ttacaaatgt aaactttata
    13921 ggtggatgtg atggaaattt aaaagggcta aaagtattag ttgaaggcat gaatatagaa
    13981 gatgtaatac aaaagttaaa aggaatagag tgtaaaacaa agccaacatc ttgcccagac
    14041 caactgtctc tagcattgga aaattatatg aatataaagt aatacataaa acaatcgatt
    14101 agttaggagt ttaattatga aaccaataat agggatttta ggaaatttaa taataatgga
    14161 aaatggtatg tttccaggtc ttgaaaggtc atatgtaaat aatgattata taaatgcagt
    14221 gctaaaaggt ggcggtagtc cagtaataat tcctgtaaat acagataaag aagttataaa
    14281 aaaacagatt gaaatggttg atggagtctt aatatctggg ggatgggata tcaatcctca
    14341 actgtatgga gaagaaacaa gagaagaaac gacatttata tatccagaag tggatgaatt
    14401 tgatttaatt gcaataagta tagcattaga acttaaaaag cctatattag gtgtatgtag
    14461 gggactacaa attttaaatg taagtttagg aggaacttta tatcaagata ataatcttat
    14521 agaaggaagt tatataaaac atacacaatc ttctaaaaga catgtagcta ctcataaagt
    14581 cgatgttaaa gaaggaagta ttttagaggg aattttagga aaacaattac ttacaaatag
    14641 ttatcatcat caaagtgtca atcagcttgg aaaaggatta aaagccatag catactcaaa
    14701 agatggtata atagaagcta ttgagaaaga ggaggaaaat tttgtagtag gagtacagtg
    14761 gcatccagaa atgatggtag attactgtga taaaatggaa aaactattta aatattttat
    14821 aaatatttgt tcaaatttat aatatacctt ggggggattg aacatgaacg aaaagaacaa
    14881 aatgggatta ataagtatta tacttttagg tattaatgca gtagtaggag ctggagtttt
    14941 tctattacca ggagatgcaa tgaaatcatt tggtgtagca agtatatttg tatatatatt
    15001 tgatatgttg ttagttttat ctatggcatt ctgttttgct gaggttgcag gtaaattcaa
    15061 taaaaatggc gctgcctatg tatataccaa agaagctttt ggagattttt gtggttttga
    15121 agtaggtctt atgaaatggg taataggatg tatttcttgg ggagctttaa tagtaggttt
    15181 tcctacatca ttatcagcag tatgggctcc agcaggtgaa ccacatatac aaaaaattat
    15241 aattgtagcc atgatagttg ggcttacaat tataaactta ttaggtgtat ctctatcaaa
    15301 aattgtacaa aatgttataa ctgtaggtaa actaattcca ttaatactat ttataggaat
    15361 aggtatattt tttataaaag gagttaattt tacttcttca acaatggtac ctccaggagc
    15421 tggagcaaca gagtttggag cagcagcact tttaatgttt tattcattta caggatttga
    15481 atctatagca gttgcagcag aagatatgga aaatccacaa aaaaatatac caatagcaat
    15541 tataagtgta atagtaattg catcaataat atatatatta aatcaagttg tatgtgttgg
    15601 tatactagga gattcgttat ctagtacatc tacaccagtg gcagatgctg caagaatatg
    15661 ctttggaaat atgggagctg gattggttac ctttggaaca ttggtttctg ttggtggaat
    15721 ttgtatgtgt ggtgcttttg taaatccaag aagttgtgta gctttagctg atgataaaat
    15781 gttaccaaga atatttgcaa gaaaagataa aaaaggtaca ccatatgtag cgataatagc
    15841 tactatgtta atcactatac caatagctct atctggaagt tttgctgaat tagcagcaat
    15901 aagtgcagtt gctagattca tacaatacat accaacatct ttatccgtat tagtgtttag
    15961 aaagaaaaga ccagaattag taggtacttt taaaactcct tttggagcag tgattcctct
    16021 aattgctgta tgtgttggaa catggttgtt attccaagca tctatgcatc aattaattat
    16081 gggattagga gctttggcaa ttggagtacc attatacttt ataatgaagt cttataatag
    16141 aaaagtctgt ggtgaaaatt taaagaaaat agtttaatta tgttataaga atttattgag
    16201 ttaaaattag acattggcag aaaattaaat acaaataagt atctataagg ctatattaaa
    16261 atattattaa ttttttattt taatatagcc ttatattgtt atcaataaat gaaaaatatt
    16321 gtaacaacag aagaacaata gtattatgtt tatagataaa aaacttatta ggaggtaaat
    16381 ttatgccaac aataacatta aaaaatggtg tagacgtact tattagagaa ggtgtaagag
    16441 aagatgctta aagcattata gatttttata atgaagtggg tggagaaact cattttctgt
    16501 catttggaaa ggatgaatat aaaatatctt tggaggaaca agaaaatgct atagaatcag
    16561 caaaagcatc tgataattct gttaaactga ttgcatttat agatgatgaa atagttggga
    16621 ttgcaacgat tgattctaat caaaaagcaa aaggaaagca tgttggagta ttaggaattg
    16681 ttgtaaaaga aaaatattgg ggaattggat tgggtaaaag gcttatgtta gaccttatag
    16741 aatggtgtaa atcaaatgga ataaccaaga agataactct tgtcactaat gaagaaaatt
    16801 ataatgctat aggattatac aaaaaagttg gatttgaagt agaaagcata ctaaaaaaag
    16861 aatgttatta taatggtgtt tatactgatt taataggaat gtcactttta ttgggaatat
    16921 aattagtgta cattttatct cgaaaaaagt atattaagta tggtataatt aaaaaagaat
    16981 ataacttaat ataattttgg aggacgaatt atgaaaaatc tgtgtaaaat atcagatgat
    17041 tatttaaaca gcaagcttaa gtatttaaag ttattatcaa aacaatatcc aagtatatca
    17101 aaagcaagta cagaaataat aaatttagag gctatattaa atcttccaaa gggaacagag
    17161 cattttataa cagatgtaca tggtgaatat gaaccatttg tacatgtatt aaaaaatggt
    17221 tctggtgtaa taaagagaaa aatagaagaa ttgttttcaa atacaataag agacagcgaa
    17281 aaaaaaatgt tagcaactct tgtatattat cctgaacaaa agttagacct aataattaag
    17341 caagaggaaa atatagatga tttttataga ataaatattt atagacttat agaactttgt
    17401 aaatatgctt ctagcaaata tactaggtct aaagttagaa aactgttacc agaaaatttt
    17461 aaatacataa tagaagaact tttgcatgag catgttaaaa gtgaacataa agaagagtat
    17521 tataaaagta tagttgaaac aatagttgac ataggaatag ctaaggagtt tataattgct
    17581 atttctacag ttatacaaaa attggttgta gatagacttc atgtaatagg agatatttat
    17641 gatagaggtc caagaccaga tataattgtc gataagctta ttgaacatca ttgtgtggat
    17701 attcaatggg gaaatcatga tatattgtgg atgggtgcag cctcaggtga aaaaacttgt
    17761 atagcaaatg cactgaggat atcagctaga tatgcgaatt tagacattgt agaagacata
    17821 tatggaataa atctgttgcc tttagccact tttgctatag aaatgtataa agatgaccct
    17881 tgtaaagaat ttattccaaa ggttaatgac caaagtgtaa caacaacaga aaagtcttta
    17941 atggcaaaaa tgcacaaagc aataagtata attcaattta agcttgaagg tgaggttata
    18001 agacgaagac ctgaatttga aatggaacat agacttttgc ttaatatgat aaactatgat
    18061 gaaggtacga ttactttaaa aggtaaaaca tataaattaa aagatactta tttacctaca
    18121 atagacaaaa aagacccata taaattaact atggaagaaa gaaatgttat tgataaatta
    18181 gtatcttcat ttagaggtag tgaaaaatta caaaaacatg tttcattttt gttttctaaa
    18241 ggaagtatat atttaaaggc taactcaaat ttactgattc atggatgtgt accattaaat
    18301 gaagatggaa gctttatgtc tatgaatata atgggaaaag agtataaagg taaggctctt
    18361 atggataaga tggagtctct agcaagagaa ggatttttct ttaaagataa ggcagaagaa
    18421 aaactatatg gtatggatat tatgtggtat ttatggactg ggaaatgttc atcattattt
    18481 ggtaaagatg atatgactac atttgaaaga tactttatag aagaaaaaga gactcataaa
    18541 gaaaataaga atccatattt taaacttaga gaaaatgaga tggcttgtaa aagattattt
    18601 gaagaatttg atttagagct tgatgaatcc catataataa atggtcatgt tcctgtggag
    18661 agtaaaaatg gagagagccc aataaaagct aatggtaaaa ttctcgttat agatggtggt
    18721 ttttctaggg cttaccaaaa aactactggt atagctggat acactttgat atacaattct
    18781 agaactttgc aattagtatc gcatgaacca tttaattctg ctgaagaagc cattgcaaat
    18841 gaaagtgata tcttatcgac aactgttgta gtagagcata aggcaaagag aaaaatggtg
    18901 agagatactg atgaaggagt aaaaatacaa gaggaaatag aagatttaaa actactttta
    18961 atggcttata agaaagggct aataaaagaa atgtagatat tatagaggca cttcataatt
    19021 gaagtgtctt cttgttaata cttattaaat atattataaa gattaataat agtagtttat
    19081 attatactta ctaagtgaat ccaagataaa tgggttcact ttttttattt ataaacatta
    19141 ttaaaaaaat atagatgttt tggttatatt ccttgtcctt tataaataga attatataaa
    19201 caaaggagga ggacaagaga tgaataaact ttctgatgaa ataataaact ctctttctac
    19261 aactataaga gaaaaggtag aaaaagtctc aaacaataat ctgaacatag aagaaattag
    19321 gttacgctca caaaaaccat tgatactaaa tgcaaactct aaagattatt tttacaatca
    19381 aaaaactatg acacttgatt taaatcaaca aaactcatat gtagtaacaa gagaagatgt
    19441 agagcaaact tttcaaataa tatgtaaata ttcaatacat tcatttatgg atgacataaa
    19501 aaaaggattc ataactttga gaggaggtca tagagtaggg ttagttggaa aagcaatagt
    19561 agaagatgga caagtcaaaa atataaagca tatatcatct ttaaatataa gggtatcaag
    19621 ggaaattatt ggatgctcgg ataagatttt aagtcatatt ataaagggaa aaaatcagat
    19681 taataatact ttgataatat caccacctca atgtggaaaa acaactttaa taagagatat
    19741 agtaagaaat ttaagtaatg gtaatgaaga ttatggattt aaaggtttaa aagtagcttt
    19801 ggtagatgag cgtaatgaga tagcaggagc ttatctaggt gttccacaaa tggatgtggg
    19861 aataagaact gatataatag aaacttgccc aaaagatttg ggtattacga tgcttttaag
    19921 gtctatgtca ccaaatgtta ttgtgacaga tgaaattggg agtgaaaaag aaataaaggc
    19981 attatacaca gcattaaatg gaggtatagg attaataact actgtgcatg gagattcaat
    20041 agatgatata caaaatagaa aagaattaaa tagacttctg gataaagagt tatttaaaaa
    20101 ggttataatt ctttctgcaa aaagaggagc aggaacgatt gaaaaaattt atgatttaga
    20161 agaaaagaga tggtattttg caaattaaaa taattattat agctctcttg gtaggaagta
    20221 gttatttgat aggtgaacaa atatacaaaa cttatactcg tagacataaa caattaaacg
    20281 atttaataag agttttagaa attttgagga tggatttatc tttcggactt tatacattag
    20341 aagaaatctt taataggata ggtggaaata aagagttttg tttttggaaa tttttctatc
    20401 aaatatcaga aggacttcac aatgaacaaa gtaaaacttt ggaaataata atatcagaaa
    20461 atattgatgt cttaagtaaa gaaacttatc taggcaataa agaaattgag gaactgaaaa
    20521 atctaatact tactttaggg aaaagcgata tagagtcaca acaaagaatg atagatttat
    20581 ctatagaaaa tttgaaaaaa caaacctatg agacaaagga agatataaac aaaaaaggcg
    20641 tattgtataa aaaattagtg acattcatag gtataggtat atgtattata ttgatttaat
    20701 atggggaggt ttattatgga aatatcattg atattaaagg ttgcaggtgt cggtattcta
    20761 atatcagtat tgaatatgat attggaaaaa acagatagaa aagattgggc aggtcttact
    20821 acattagcag gtgtaataat cgtattgggt atggtaataa cagaaataag tgacttgttt
    20881 aatactgtaa gaacaatgtt tcaactttac taaaaaggag ggagtaattt ggaaataatg
    20941 caattaatag gaattgcaat catttcaact acactctgtt tagtaattaa aaaagataga
    21001 ccagagatag caaatttcat agcaataata actggagtaa taatactatt gtcagttatg
    21061 tttaagctaa attttatagt agatagtata caagatttgg ctaacaaggc taacataccg
    21121 actatgtata tatcattaat aataaaatta attgggatag catacttaat ggaatttgcg
    21181 atacaacttt gtaaagattg tggggaagga aacattgctt caaaactaga atttggaggt
    21241 aaaataatag ttatgtcaat gtctttccct atacttttgt ccattgtaga aatggtggtg
    21301 aatataattc catagatgtg tatcttggaa tgaatataag tatattagag aactttcgtt
    21361 gtattgaggt ttatagagtg aagagaggca tggttgctta tgaaaaaaag atttttacca
    21421 atgattatag gttttttatt tactttcttt tttgtaaata tctttgctat tatgatattt
    21481 gctaatgaag taccaagtag tgaagataaa gaatatggtg aaacaaaaaa tagcatagat
    21541 aaatatatag atggtcaatt agacaagcta gatataaatg agattcaaga ctatataaat
    21601 aaagaaatag ttattaatga tgttaattta aaatcatttg taaaagattt aattagtgga
    21661 gaaaaaaata tcttagattt attcaataaa gatggattga agatattgat gtttgatgaa
    21721 tttaaagcaa gtttaaaggt agtagcagta attttggtac tggctctgtt atcatctatt
    21781 ttaaagagtc tggaaaactc tttttcatca ggtgcagtaa gtcaaatagc aacatatata
    21841 atatttatca caatggtatc acttacactt attggtttta aagatgtatt acagatttgt
    21901 tatgatgcaa tagaccatac tgtaggtctt atgcaagtta taatgccaat attaataaca
    21961 tttttacttt taattgggtt cccaataacg tcgactactt tgaatcctat cttcatagga
    22021 ggagtaacat ttataaatgt attttttaaa aattttctat ttgtatctat tactgttgca
    22081 tttggaattt taataataaa caatttatca aaaaatataa ggctaaagag atttttctcc
    22141 tttgtaaaac aaataaatta tgtttctatt ggagctatgt ttactgttta cttaggtctt
    22201 gtatccatac aaggattgta tgtaaccagt tttgataaat tcagtgtaaa gactgctaaa
    22261 tttgctatag gtaattttat tcctgttgta ggtggatttg tatctgactc tgtggatatt
    22321 ttattatctt catctcagct tataaaaaat atatttggag gaatagggct tatcttactt
    22381 gttggaattt gtttacttcc agtaataaaa attttatcag taatagtagt gtacaaatta
    22441 gcagcaataa ttgtagagcc tgttggagag gatggaatat ctaatttttt aaatgaagtg
    22501 gcaaatttaa tgataataat gctagcatca gtaattgcta taacagtaat gttctttgta
    22561 actgtagcta tcttgacctc gataagtgtc gtaagtcaag ggtaatttat atatcagtga
    22621 aaaataatta catttaatct aatgttttta gagaggagga tgctatgtta gagggcataa
    22681 aggcatggat agttagtgtt ttgataggag catttatagt aaatatagta gatatgattt
    22741 taccaagttc taagataaaa ccttatgtca acctagtttt aaactttatg tttgtattta
    22801 ttgtaataac tccagtagta ggattttttt caaaagatat gagtttagaa gatagaatat
    22861 taaaatctat gggaaactac aacaagcagt atgtagatag tacaaatgct ttggcaaaag
    22921 agactggaaa taatagtcta tccaagggat atgaggatgg gttaaaagaa gtacttaaat
    22981 taaaacttga tgaatatggt tatgatttag aagatataga gcttaatggg gcaaatataa
    23041 ataatataaa aataaaagaa aagaataata gtgcaaaaag taataacagt tccaatgaag
    23101 aaaataaaaa taatagtact aaaagagagg aagaaaacat aaactctaat gacaaagaaa
    23161 attctaaaca agtcttcaaa aaaggtacag agtatgggtt gaacctaaat gaagaaaaat
    23221 taaaaaacga cttaataaag gtgctggatg tttctataga agatatacaa attgataaat
    23281 aggagttggg agaatgttga aaaacttaaa tgacaaagat aagagaaagg tatattcact
    23341 aataactata gcaggaatat gtgtggtttc attagtatta ttatcttgtt ttcctagtag
    23401 caaaagtgat aaacaagttg gaaaaacaga gactaataaa aatgctgaaa agcaagtcac
    23461 taaaaaacaa gaaaaagatg atttagagtc aaaattaaca gcaatactat ctaaaattga
    23521 tggtgcagga gacgttgatg taatggttac atttgaatca agtgaagaaa tacaacctgc
    23581 ttttaattca aataatacaa cagaaacaac agaggaaaaa gatgcacaag gtggagaaag
    23641 aacagttacg acttctagtg aaaataaaac aatgataact tcaaattcaa gtgaccctgt
    23701 agttataaaa actacagagc caaagataaa gggagtgata gtggtagcaa gtggagcaag
    23761 tgacccaagt gttaaagaga cactttatag tgcagtacaa acatcattac aagtagctgg
    23821 tcatcaagta gaaatatatt ctaaataaat acatataacc ggggggataa atgaatgaag
    23881 tttaattata agggaagagg atttgtaata ataactttaa ctgcaatgtt agtggtagta
    23941 ggaacagtga attatcaact aagtaagaaa tctttattag aaacatcaaa agagtttaag
    24001 gcatatgaag aagcacagtt gcaaaaaaat actgatgata gtgatagtag taagacagag
    24061 gattctagta atgtagataa acaaggtggt aaggaaagtg ctgatattga tatagtcgat
    24121 agcaaagcta gtaaagtaaa agaaaaggca actgagacaa gtaaggaaat aaaagctcaa
    24181 ttatcatcag agaaaaatat gaaaaaggca tcttatattt tagatatgaa aatgaataga
    24241 gaaaaacaaa gaaatgagtt agttcaagat ttaaatgaaa tgataaataa tccatctaca
    24301 acagaagaat ctagaaaaga agcttctaat atgaaattaa atatagttaa aattcaagaa
    24361 aaagaacttc aaatagaaaa ccttttgagt acaaaaggat atgaagaagc gttagtttat
    24421 ataagcgata acaaagttaa tgtagttgta aatgaagcta aattagaaaa gaaagatgca
    24481 gcaaaaatat ttgatttagt tgcagaacaa gccaatgtga agtatgaaaa tattaaactt
    24541 acaaataata atagtaataa gtaaagtata aacctcagaa ttaactgagg ttattttctt
    24601 gcaaagtaga cttgtatttg gtattattat acttataaaa atagttcctt aaaatcagga
    24661 ggtaagtttt aatatggaag ataataaatt tggacaagta aaaatatcta atgatgtaat
    24721 agctacaata gctggtttag cagcattaga agttgaaggc atagaaacta cagcaacatt
    24781 aacagataag ttgttaaaaa ataatggagt aaaaatacaa atcgaagaag aggatgttaa
    24841 tttagatgta atggttacga taaagtatgg aatgtcaata ccagatacag cttttaaagt
    24901 tcaagaaaat gtaaaaaata ctgttgagac aatgacagga ctgaaggttt ctcaagtaaa
    24961 tatacacatt caaggaatca gctttaagaa ggataaagtt gataaagaag aagcaaaagc
    25021 aactaagaaa aattaatatt agccctctga aaagtcagag ggttttttgc tggtaattga
    25081 ttttatatgg ataattaaac ttaaaggaga attttgattg atgaaaaaag atagggcaca
    25141 aaagagtaca acaagagaat atataatgaa acttatatac caaataaata ttaataaaga
    25201 agattttgaa actttagaag ataaagtaga taatttttta aaagataatt cagaacatat
    25261 tataaataga tataaagaac ttgcacttca atactcaaaa aatacaaatt taaagttaga
    25321 agatactgaa attgaagatg ttatagacaa gaaatatata aatacagtat gcaaagcatt
    25381 aaaagaaaat catgacaaaa tagatgagtt aataaataag catgctaaaa actggacagt
    25441 agatagaatg ccaaaggtag atgtttctat acttagattg tctgtgtgtg aaatacttta
    25501 tttagacact cctaataaag tatcaataaa tgaagcggta gaacttgcaa aaatatattg
    25561 tgatgataag tctcctaaat ttataaatgg tatattggga agtgttgttg atgaaattgg
    25621 gaaataatat aataattgga attgatacta gctgctatac tacttctata gcagctatct
    25681 ctttagataa gaaagttata ttcaatgaaa aaataatgtt agaagtaagg gataattcaa
    25741 aaggtttaag gcaaagtgaa gctgtatttc aacatatcaa taatttaggg attcttagtg
    25801 ataggataaa atcttttaaa gataagttta atgtagaagg agtttgctca tcaaaaaaac
    25861 cgagacctgt tgaaaactca tatatgcctg tattcaatgt aggtcataat tttggaaaat
    25921 tattgtctag tatatatggt tgtaggtttt atgagactac tcatcaagag aatcatattg
    25981 aagcaagttt attaaatagt aaattaaaaa ataataataa atttatttct gttcatatgt
    26041 ctggtggtac tacagagata cttttaacaa gcaaacaaga tagtcatcac aatgtatgtg
    26101 atactaattt aggtaaaatt gccaaaataa gtattaaaaa agatgataaa agtaagttat
    26161 acaataattt tggatataat atagatataa ttggaggaag taaagatatt agctttggtc
    26221 aactcataga tagagtagga attaagcttg gatataaatt tccttcagga aagtatttgg
    26281 atgaaaatgc tttaaattgt aatcttaaaa tagaaagtgg tctgaaaact tctgtaagag
    26341 atgggtatat gaatttatct ggattagaaa atcaggtaaa taagattata aatgataatg
    26401 gagataatac taatcaaaaa gaatacatat ctaaattagt attagattca gtagttagaa
    26461 atatgtttaa atctttagta tatttatgtg aaacttataa tgtaaatgaa gtagtatttg
    26521 ctggtggagt ttctgcaagt aagtatattc ttagagaatt aagcatgaag ttaagaaaaa
    26581 agcatataga agcttatttt acagaaccac aatattcaac tgataatgct gtcgggtgtg
    26641 ctataatagg attgaataac tttttaggag aaagagtatg aaactaagag ctttagatat
    26701 aagtgaagca aactcatata ttaaaagaat tttaataaac gacccaatac tttctaatct
    26761 taaagttaaa ggagaaattt ctaattttaa ggttcatagt agtgggaatg tatatttatc
    26821 acttaaagat gaaacatcaa agttaaactg tgtaattttt aaaagtaatt ttaatcgcaa
    26881 tttaaaatta gataatggtg taaaaattat agctaatgga tatatatcag tctatgaaag
    26941 agatggtgcg tatcaattat acattaatga aattgaaata gagggaatag gaaatttaca
    27001 catagagttt aatagattaa aggaaaaact taataaagaa ggattatttg accctaaata
    27061 caagatacct atacctaaaa tgccaaactc aattggcgtt ataacatctc caacaggtgc
    27121 tgttataagg gatataataa atgtaataaa aagaagatat ccgaaagtta atataaagct
    27181 ttatccagta atggttcaag gagataaatc agctgaagaa atctgtgaag caattagatt
    27241 tttcaatcat atgaagaatg ttgatacact tatagttggt agaggtgggg gttcaataga
    27301 agaactttgg tcttttaatg atgaaatggt agcaagagag gtttttaatt ctcaaattcc
    27361 gataatatca gcagttgggc atgaaacgga ttttactatt tgtgactttg tgtctgatat
    27421 gagagcccca acgccatcag cagcagctga gattgctact ccatcattgg atgatataaa
    27481 ctataaactt ggaaacataa aaagtaggat gagtaagtct cttacaaatc aaatagagct
    27541 agaccaatat aggttagaaa cagtatttaa taagataaat aactatttag attcgtacac
    27601 aataaaagat aaagttatac aattagacaa aatatatgat aaaataattt ttggaataga
    27661 aaataattta aaattagaag atgaaaaatt agtaaaaatt ggagcattac tccataattt
    27721 aagtccatta gctactatgg atagaggtta tagtataact caaaaaaatg gaaaagtaat
    27781 aaatagtata aaaggattaa aaataaagga tagtatagat atagtattaa aagatggaaa
    27841 tttagaatgt atgatagata aaattgaaaa taaagaggga tagtatgaat ttaacctacg
    27901 aagaagctta taaaagatta gaaagtattt taagtgaatt agaatctaaa aatgctagct
    27961 tggatgaatc tttaagccta tatgaagaag gtataagcct ttataaacat tgtaataagc
    28021 ttttagatga tgctaaattg aaaataagta aatttaatca attaggaata gaagaagact
    28081 ttaaaataga ggaggaataa aattggaatt taaacaatgt ctaaaagaaa aagcaagttt
    28141 tgtagaaaaa gtacttaaag aatatatgcc taaagaagaa gggtatcaaa aaactgttat
    28201 tgaagctatg aactatagtt taagtgctgg aggtaaaaga ttaagaccaa tacttacatt
    28261 ggaggcttgt aaaatagttg gaggaaatga agatgaagct attccttttg ctattgcaat
    28321 tgaaatgata catacatatt cactaataca tgatgatttg ccagctcttg acaatgatga
    28381 tttaagaaga ggaagaccaa caaatcataa agtatatgga gaagctatgg ggattttagc
    28441 aggagatgct ttgttaaact atgcttttga agtaatgctt gcaggctcaa tcaataaaga
    28501 aaatccagaa aagtatctaa aagctataaa tgaaattgcc aaaggtgctg gtatatatgg
    28561 aatgataggt ggtcaagtag tagatgtaga aagcgaaaat aaacaaattg aaaaagaaaa
    28621 attagattat atacatatga ataaaactgc tgcaatgatg gtaggctgta tgagagcagg
    28681 agctactata ggtggagcaa attctgaaca aatggaagaa ataactaaat atgcaaaaaa
    28741 tatcggatta tcattccaga tagtagatga tatactagac atagtaggag atgaagctaa
    28801 gttaggtaaa aaggttggaa gtgatataga aaatcataag tcaacatacc catctctttt
    28861 aggtttagat aaatctaaag aaattgcaca taatttgatt gatgaagcaa agaaaagtat
    28921 agaaaaatta tctgatgatg tagatttttt aaagggattg gctgaatata taatagatag
    28981 agaatactaa aatatacagc taaaatcgag gagggaaaaa tggatttttt ttcggagatt
    29041 tttaacaatg gggctttggg aataagtctg attgcttgtt tcttagcaca atttataaaa
    29101 atatttactg gtaaggaaaa aagaattgag ttatcaagaa tacttatttc aggaggtatg
    29161 cctagttccc atagttcttt tgttacaagt ttagctacag tggtaggtat tgaaaaaggg
    29221 tttaattcta ctgattttgc tataattacc gtacttgcct taattataat gtatgatgca
    29281 gcaggtgtta gacgagctgt tggtaaacaa gctactatat taaatcaaat ggtggcagat
    29341 atacagcatg gaaagcatat agagcagaag aaattaaaag aattaatagg tcatacacca
    29401 ctagaagttt ggtttggagc tttacttgga atagtaacag ctttaatatt gatgtaaatt
    29461 gaaaatttac agaataaagg tgaggataat atgtataaat atttagataa agtaaattct
    29521 ccaaaggata taaaaaatat gagtatagaa gaaatggatt tacttgcaaa ggatataaga
    29581 aagttcttag taaaatctgt ttcaaagaca ggaggacact tagcttctaa tctaggagtg
    29641 gttgagttaa cattagcact tcataaagta tttgatagcc ctaaagataa gattgtatgg
    29701 gatgtagggc atcaatctta tgttcataaa atagttactg gaagaaaaga ttgttttgta
    29761 tctttgagac agtttaatgg actaagtggt tttccaaagg agaatgaaag tcctcatgat
    29821 atttttgata caggacatag cagtacatct atttctatag ctacaggtat tgcctgcgct
    29881 agggatataa aaaaagaaaa ctatagtgtt atatctgtga taggagatgg ttctataaca
    29941 ggaggtatgg cacttgaagc tttaaatcaa ttaggatata tagataccaa tatgatagta
    30001 atccttaatg ataatgagat gtccatagat aaaaatgttg gaggaatgtc taaatatctg
    30061 tcaagtatta taagaaactc aacagttgag aaaatgacag atgaagttga taaaatttta
    30121 aatgttacac aaacaggtga aattttatct aagactgcac atagatttaa agataaacta
    30181 atgtatagct tttctcctca ggactgttca ttttttgatt cattaggtat aagatattat
    30241 ggacctatag atggccataa tactaaagaa ctaatagata tattgagaaa agctaaacac
    30301 aaaaaagggc ctgttctttt acatgtaatt acgaaaaaag gaaaagggta cagatttgca
    30361 gaagaacaac ctgataaata tcatggagta tcaaagtttg atataaagac aggtgttaca
    30421 tctgcaaaag ttaagtctat gtcaattagt gttggagaaa aacttgttga tatggctaat
    30481 aataatgaag atatagtagc aataacagct gcaatgccat ctggtacagg gttaaacctg
    30541 tttgaaagtg catatccaaa aagatattat gatgttggta tagctgaaca acatgccaca
    30601 ggatttgcag caggtcttgc aaaaaatggt atgaaacctt actttgctgt gtactcatca
    30661 tttttgcaaa gggcatatga ccaagttatt catgatgtat gcataacaaa aaagccagtt
    30721 acttttctta tagatagagc tggattagtt ggaaatgatg gagaaactca tcatggtatg
    30781 tttgatttaa gttacttaaa ttctattcca aatattgtag taatggctcc aaaagacaca
    30841 agagagatgg agcttatgat ggatttgtca ttaaaattag attgtccact agcaattaga
    30901 tacccaagag gaagtagtta ttacctagat aagggagaat atggagagat tgtactgggt
    30961 aaatatgagg tattagatga cggtcaagat acagtcatac tttgtattgg aagtatggta
    31021 aaacatgctt tagaggctaa agaaatatta tcaagagaag gaataaatcc gacaatagtt
    31081 aatgctagat tcttaaagcc aattgatgaa ggtatgttaa aagcattatt aaaaaatcat
    31141 aaaaatgtag ttactataga ggataatatt gtaactggag gatttggaag tagaataaac
    31201 aagtttatta tagataatga atataacgta aatatactta atatagcaat accagaagaa
    31261 tttgtaaaac atggcaatat agatgaatta tatgattttg taggattatc gccaaaaagt
    31321 attgctgata aaataaggaa attagttatt gagtaaaaat ctaatgtaga tattgtaaat
    31381 taaaatttat ataaaatata gaatttatca attagaataa atatcaagtg tgttacttca
    31441 agctacttta gttatcatta aagtagcttt taacagaaag gtaattgtat gaaaaaaaga
    31501 atagatttat tattagtaga acaagggtac tttgaaagta gagaaagagc caaaaaagca
    31561 ataatggctg gattggtttt tgttgataat caaaggtgtg ataaggctgg tacagaagta
    31621 aaagaagatt gtagtataga agtaaaagga aatccaatac catatgtcag tagaggtggt
    31681 ttaaagcttg aaaaagcaat gaaaaacttt gatttgacta ttgatggaaa ggtatgtatg
    31741 gatatagggg catccacagg tggttttaca gattgtatgc tcaaaaatgg agcaataaaa
    31801 gtattttcta tagatgttgg ttatggtcaa cttgcatgga aattgagaca agatgatagg
    31861 gttgtgtgta tggaaagaac taatataaga aatgttacaa tagaatatac aaagcagttt
    31921 gctgattttg cgtcaattga tgtgtctttt atatcgttaa agttagtact tccaaaggca
    31981 aaggagttag tacgtcatga tggggaagtt gtcgctttaa ttaaacctca atttgaagca
    32041 ggaagagaaa aagtaggtaa aaaaggagta gttagagaaa aatctacaca tatagaagtt
    32101 ataaaaatga tttctgattt ctcagtcgaa aatggatttg agattttagg gttagatttt
    32161 tctcctatta aaggtcctga aggtaatata gaatatctaa ttcatttgag aaatggaaat
    32221 gaaggatatg aatttgatgg tgagacatac aataacaaaa tagtagaagt tgttgaagct
    32281 tctcataatt tagacaaata ggttaaaagg gggcttagat ttgatcctcg aattatatat
    32341 gaaaaattgt gccttagttg aagaactaag gttgaatatt gataaaaact taaatatact
    32401 tacaggtgaa actggttcag gaaaatctat aataatagat gcacttggac tttgtttagg
    32461 tgataaatat gataggtctt ttttaagaaa aggaacagat aagggacttg ttgaggctgt
    32521 atttttttca gacaatagat atcttaaaaa aatattagaa gaaaatgata taagtatgga
    32581 agatgataat ttattggtaa taactaggtt gatatactca gatggtaaga gtactgctag
    32641 agtaaatgga aggactgtta aagtgtctct tcttaaagaa attgcatcta cacttataga
    32701 tatacatggt caacatcaaa atcaagcttt gttcaataaa gatactcatt taaaatttct
    32761 agaccttttt ggagaaaatg aattggaaga gtttaaaatt gcttataaaa atgtgtacca
    32821 taagtatagt gaagtaaaaa aagctttaaa ttgcttgaca gaaaataaag atgagatgca
    32881 aattcaacga gaaatagatt tattaagatt tcaaataaat gaaattgaag ctgcaaatct
    32941 aaataagaat gaatatgaag atttattaaa acaaagagaa gtttatagaa atagtgaaaa
    33001 gatatataat aatctaaatt cgagttatag caagttacat aatggtgagt ataatgttat
    33061 tgacctaata ggtcttgcat ctaaggaact taatgacata tctaaatatg acagtgtttt
    33121 aagtgaatat agtgatactg tagaaagaat tatgtatgag ttacaagaca tatctggtga
    33181 gataagaaat tacaaagata atatagactt tgaaccttat gagctagagc agattgagct
    33241 tagaatcgac gaaattaata atttaagaag aaaatatgga gacagtatag atgatatatt
    33301 tgagtattat ggaaaaatta aagatagatt agatgaaata ttaaatagag atgaaagagt
    33361 tgaacagtta aaaagcaaac tgatgaatat agaagaagat ttaaaaataa aagccagtaa
    33421 attgactaaa gcaagaatag aagtagcaac tactttagaa aaaatacttc ttgatgaatt
    33481 aaaaagctta aatatgaaaa atgtaatgtt caaggttaat tttgaaaaag gttcatttac
    33541 tttagatgga gtagatgata ttgaatttat gatatctttt aacttaggag aagatattaa
    33601 gcctatatat aaagttgcat caggtggtga gatgtccaga tttatgttag cgtttaaaac
    33661 catattggct gatatagatg atattgatac attagtgttt gatgagatag atactggaat
    33721 aagtgggata gcagcacaga tagttggtga gaaattgagt gatatagcaa agaaaaaaca
    33781 gataatatgt ataactcatt tacctcaaat tgctgcaaat gcagacactc attattgtat
    33841 agagaaagat acttcaaata ataggacgtt taccaatgtt agtaagttaa atgaatctca
    33901 acgaaaaaat gaaattgcca gacttatagc aggtaacaat ataacagaaa aaactattga
    33961 acatgcaagt gaaatcattg aattggctaa aaaatgttag tgaaaaacaa gcattttaat
    34021 aggtgtaaag aatttttata gaaatgcttt acacctattt tttatgatga aattatttta
    34081 aaatatctag tgcctatatt gaagaaaagt aggtttgaaa atggaatgaa ctttgtataa
    34141 atttgtaact ttgaaatttc taaatgctta agtataatat tatttattaa ggtagtagtg
    34201 aggggaggca taaaatgaat aaaaaagata ctatgcactt aatttttata attgtaattt
    34261 ttattttatt atggtatatt gtggtagcag taagtgagat taataaaaat attgaaagtt
    34321 gtagtaacaa attagattat ttgtcacaag agcttatgag tactaagtcg gatattagta
    34381 atactataaa tgaagaaatg tcaaaaagtt atataacaaa aagtattgac ttaaaagtaa
    34441 aaaaaatcga gaaaaaagaa tgtactattg atgtagatgt tcaattaagt aaacttggaa
    34501 aaaatggaaa agtatttttt atgtataaag aagatagtga taaatggaat gaaactgaga
    34561 tgacaaaaca tggggaatta tcatatgcat gtgaaataaa gataaataca ggaagtgaat
    34621 atgattataa agtagttaca agtggagata tatctgaaag ttctgatgta caaaaactga
    34681 ctaaatctga ttatatgcct gaacaaccta tatttaattc tgggataaga gataatagag
    34741 aatactttat tgaaatagta gaaaatagta atctttttaa tcatgagagt gaaaagtcaa
    34801 aaaataaagc ttacgataat atcaggttag aaaaagtaga tattattgta aatgaaggaa
    34861 aaaaagacac tatatacaaa gcccaattag ctgaaggctt agatgatggc aaaactaata
    34921 attcaaatag aaatcaagtg aattatgaag taagttttcc taaaagggat attgatgaca
    34981 taatttatat taaagctaaa cttacataca ataatggagt agaatataca aaggatataa
    35041 ctgaagatat aactatggga ttacaagatt tagaaaaata aatacatatt taaataaata
    35101 ttcaaataga tttgttcaca aatagagtat aaataaaact gtagtagttt tacttatact
    35161 ctattaaaaa attataattt taattccata tatctagccc cttcaattgg attataagta
    35221 taaagttcta tatcataaaa accaaacttt tcatataatc cttgtgcact tttcatgctt
    35281 ggaagagtat ctaatctcat gtgtgtatat ccaatttttt tagcttctac aatgatttct
    35341 tccaatagta tttttcctat ttttaatcct ctaaattgat ttcttacata taatcttttt
    35401 aattcacata ttttaccttc aagttttttt aaagcaacac aacctgccaa attttcatca
    35461 acaaatgcca atatcaatga gcctgaaggt tttttatatt ttcctggaag tgtttttaat
    35521 tcattattaa agtcttgaaa acataagtct atatttagag aatttgaata ttcagtaaat
    35581 agaagtttta cgttttcaag attttctaaa tctttttctt caacaaattg taaagttata
    35641 ttttgagcca ttttatccgc cattatataa acatgtttat ataatgactt cacctccttg
    35701 atatatgtat atttttctta atatcaatta gattttaata ccattacttt ttcaccacca
    35761 tagtcaagct ctccattaaa cttaaatcca aaatctttat aaagcttaat agccatttta
    35821 ttgtcttcga atatactaag atatagttca tcacagttat attcattttt aagatgattt
    35881 acaagaaaat ctaatgatgc ttttccacat cctttacctt gatattttga atcaatcata
    35941 aatctatcaa gccaaactct atcatcttct aaacgaccat acatagcaaa tcctattaaa
    36001 gaattttcat catatattcc aacaggtacc caagctgtta taaatttaga ttcagcaata
    36061 gaaagtgcat tgctttctat gtaatctaac tgacttttat ctacagacaa actaactaaa
    36121 tcgtgccaat tatttatgtc tgcaattctt acatttaatt tcaagatatt accgtccttc
    36181 aaactttttg ttacaatcta atactatcat attatgtatt aaaaatcact tattaagatt
    36241 ttaaataaac tttttgaatg aataaattct caggaaaaag gaattataaa tacataataa
    36301 tacagcatag gagtagatgg atatatgaac aaaataaata aaaatttcaa aaatatttgg
    36361 ataatattag gagctgtatc gatttttacc atggttataa tttttaataa aaattgtaat
    36421 gttaataaag agactcaaat agaagctttt acaattaaga atcatgataa aaaatttgta
    36481 tatcctatgg gaaatataat tggagtaaaa gcaaatacag atggtgtatt ggtcttagga
    36541 tatgaggaag aggatgtagg ctatattgga ggcatacaaa taggtgataa tatagtaaaa
    36601 atagataata aaaggataaa aaatagtcaa gatgtatcag agattttgaa taagattaaa
    36661 aaaagtaaag ttgaagtgac ttttgagaga aaaaataagt ataaaacaga agtaatagag
    36721 actaaaaaag aaaatggcaa gtataaactt ggattatggg taagagataa aatatctgga
    36781 attggaacta tgacttttta cgaccctagt atggaaaagt tcaaagcaat cggtcatgcc
    36841 ataaaagatt cggatactaa tgaactatta aagataaagc aaggctatat atataagcca
    36901 gaacaattaa aaatagtaaa agctagtaat gaaaaagtag gtaaaataaa aggagatttt
    36961 aatgacagca atttaatggg taatttctca aataattcag agctaggtat aagtggtaat
    37021 attacagaaa accataataa agagtttaat gtagcaaata aagaaaagca attaatagaa
    37081 gttggaagac cgcaagatgt aaaaatagga gatgcagtaa ttctttttga ggataaaaac
    37141 aaaaatataa caagctatga tataaaaata gaaagtatag tatatgataa aggaaattat
    37201 agagatatgg taataaaagt agtagatgac aagttattgg aatacacagg aggtatcgta
    37261 caggggatga gtggagctcc aataatacaa aataataaaa ttattggtgc aataactcat
    37321 gtttttagag ataatccgaa aaaaggatat ggtattttta tagatgaaat gataaaattg
    37381 taggtgaggc attaaaaaat tttattattt tatcaattat ctaggaggaa tataattttg
    37441 gagtgtcgaa tatgctttag agtagataat taggaagcaa ttgtgtaaaa agtttagttt
    37501 tctgtaataa gaagatgttt tttaatgggg ggatttttag tggaaaaaat caaaatagtt
    37561 ttagcagatg acaataagga tttttgtcag gtattaaaag agtatttgtc taatgaagat
    37621 gatatcgata tattaggcat agctaaggat ggaattgaag cattagactt agtaaaaaag
    37681 acacaaccgg acttattaat actagatgta ataatgccac atctagatgg attaggtgta
    37741 attgaaaaat tgaatactat ggatatacct aaaatgccaa aaataatagt actatcagca
    37801 gtaggtcaag ataagataac tcaaagcgca ataaatctag gagcagatta ctacatagta
    37861 aagccatttg attttgttgt gtttataaac agaattagag agctagtttc taatagagtt
    37921 acacaagttg agccaaaacc tagaccagtt caagagactc aaatgactag aagtgatttt
    37981 gttaagaatg taggaaatat agagacagaa atcacaaata taatacatga aataggagta
    38041 ccagctcata taaaaggata tttatattta agagaagcaa taaaaatggt tattgataat
    38101 gttgagcttt taggtgcagt aacaaaagag ttatatccaa gtatagctaa aaaattcaat
    38161 acaacaccaa gtagagttga aagagcaata agacatgcaa tagaggttgc atggagtaga
    38221 ggaaaagttg acacaataaa tcaattattt ggatatacgg tacacaatac taaaggaaaa
    38281 ccaactaatt cagaatttat agcaatgatt gctgataaat taagactaga acatagtatg
    38341 gttaaataaa caagacataa aaagtaaggc tttttaatta aggcattggc tataaatgcg
    38401 tattacaagc agcgaaacgg tataaccact agggttatac cgtttcgcta ttttaataaa
    38461 tataaaaatt ttctttatta tttgcttact atatcaatat aataatttta ttatactatg
    38521 gatatagtat gtgtctttac aagttgtaaa ctgacagtgg tttatttttt aatataaata
    38581 ttgactttga tgcaggtaaa ctttgtattt ttaagcgtat tatggaatat gttaaataaa
    38641 aaaatgatga aatatagtat tgtaaatgcc aaagatgcaa aacaaactta aaacatttat
    38701 tttattgtta agtaatgcta taatataatg tgattttaat aatgatagtg gaggtttaaa
    38761 tatgagagtc gaggccccta taaaagtaga tcgaaaaacc aaaaaacttg ctaaaagagt
    38821 tgaaagtggg gaaatagcag ttataaatca tatagacata gatgaagttg ctgcaaactc
    38881 tttagtagaa gctaaaataa aacttgtcat aaatgcggct ccttctataa gtggtaggta
    38941 tcccaataaa ggtccaggga tattaactga aaataatata ctaataattg ataatgttgg
    39001 agaagagtta tttgaagaat taaaagaagg agaaactata gaagttgtag atggaaagat
    39061 atatagaaaa ggaaaatttt taggtgcagg agaagtttta gacaagtatg aggtttcaca
    39121 ccaaataaag gctgcctatg agaatttagc tgtagaattg gatagattta tagataatac
    39181 tatagattat gctaaaaaag aaaaaggatt tatacttgga gaagtagaaa ttccaaaagt
    39241 gaaaactaat tatgctaata aacatgtatt gatagttgta agaggtcagg actacaaaga
    39301 agatttaagt actatgcttt cttatataga agaggtaaag ccagtattgg ttggtgttga
    39361 tggaggagca gacgctttaa ttgagtttgg atatactcca gatgtgattg taggtgatat
    39421 ggatagtgta agtgatgaag ctttaaaaaa ggctagtgaa atagtagtac atgcttatac
    39481 agatggaaga gcaccaggtc ttaagagggt tgaggaatta ggcttagatg cagttgtatt
    39541 tcctgcgcca ggaactagtg aagatatagc tatgcttatc gcctatgagt ataaggctga
    39601 gttaatagta gcagttggta cacattcaaa tatgattgat tttcttgaaa aaggaagaaa
    39661 aggtatggca agtacttttt tagttagact aaaaataggt tcaaagctta tagatgctaa
    39721 aggcgttaat ttgttataca gaagtaaatt aaaaataaag tacatatggg cattaatagc
    39781 aacagcatta tttcctgtgt tggtagttgc atcattgtca ccaggagttc aacagttcat
    39841 acaactaatg caattaaagt ttagagtatt attacagatg taaattttag gaggataaat
    39901 atgcatatta atatgaaata ttatatagtc actatagggg ctatatttat agctttgggt
    39961 ataggcatgt tagtaggatt taatttaaat aataatcaac aattaagtga acaacaagca
    40021 aacataataa atgatttgga tgataagttt aatatcttaa aagaaaaaaa tgataaactt
    40081 gacagtgact tagcaagtgt aaatagagat tatgaagagg cagttaattt tataaataaa
    40141 aatgtagata agattttagc tggttcgctg aatggtaaaa gtataggaat aatatcggct
    40201 aatgaaaatg acgattatac aaaaaatata gaagacataa taaataaatc taatggaagt
    40261 attgcattta atatagtatt aaaagaaaat ataacaaatc cagaaaagtt aaaggaaatg
    40321 tcaactaaac taggtacaga tgtaaaaaat gcaaatgatg cagttaatta tataattgac
    40381 acccttaaaa aggaagatgc aagtgacatt ttaacatatc ttcaagaact agatgtaata
    40441 aagtttaact ctcttggaaa tacatatcta aaatatgatt ctgttgtaat agctggtgga
    40501 aatgatgcaa aagacagtac aaaacagttt gaaaaaatag aaaaatttgt agtttcaaaa
    40561 cttaaatcag agaataaata tttagtagaa gttcaaaata caggagttaa aacttcttat
    40621 gtagaactat attctaaaaa caaagttgca actatagaca atattgatga aggtatagga
    40681 agtatatctc tagctattct tctacaacaa ggtaatatag ttggtaattt tggtagattg
    40741 gatactgcaa cttcattgtt gccatctatt aaatagaaat aattttaagg agagaaaaaa
    40801 tggattctta tataagtata ataataccag cttataatga agaaagtaga ataaaatata
    40861 cattagaaag catattggac attaagcaaa taaatgaaat aatagttgtg gatgatggtt
    40921 caattgacaa tactgctaag gttgtatctg agctagaaaa tgaaaagatt aaatttttta
    40981 agttagataa aaatagaggt aaaggatatg ctttaaatta tggtatcaaa ttagcaatga
    41041 aaaatgcaga tattataggt tttctggatg gagatttagg aagctctgct aaagaagttg
    41101 aaaaattaat agtacctata ttaaatggtg aggcggatgt tacaatagcc aagtttcctc
    41161 ctgcaaagag aaaaggaggt ttgggatttg taaaaggttt agctaaaaat tctgtgtttg
    41221 agatgactgg tgttgagtta gatgcaactt tatcaggtca aagaatattt aaaagagaag
    41281 ttttagaaaa gtttgatgaa ataccttttg gatatggagt agaagttgga atgaccattg
    41341 atatattaaa gtatggattt aaaattaaag aagtattagt aaatatgact cataatgaaa
    41401 caggaagaaa tttaaaaggt tttattcata gagggaaaca gtattatcac ataaaaaagg
    41461 ttttaggaca aaaaaagaaa gaatggaggt aatatcttag tgaaagatta tatactttat
    41521 accattttat tattaactgg attatttgga acctatgctg taattccact atttaaaaat
    41581 ttattgataa atggaaatgt acttagacca aactataaaa aagatatgat accagtaagt
    41641 atgggaattg tatttttgcc tatgattgta atcaatggaa taatacttgc tttctttact
    41701 acagaattta aggatttagc gtatatattt atgtttatgt ttggtatgat atccatgttt
    41761 tttgcaggga tactagatga tgtcattgga aatagagatg taagtggttt aaaggggcat
    41821 tttaaaagtt tatttaaagg aagtcttaca acaggaggtt ttaaagcttt atttggtgga
    41881 tttgtagggc ttatagtttc tgttgctata tcaaaagaca taattgatat aatagtaaat
    41941 acactaataa tagctctatc aactaattta atgaatcttt tggatttaag accaggaagg
    42001 gcaataaaag catatttatt cataatggta attatatttt taacattgac aggatttgtt
    42061 caagttcttc cattacttat agtaccaaat gtacttgcat actttaatta tgatttaaaa
    42121 gcaagggcta tgatgggaga tactggttca aacgtccttg gaatatctat tggaatgctc
    42181 atttcttttg ggtatccatt taacattaga ttaggatggt taatatttgt agttattatg
    42241 catatattca ctgagaaata ctcattaact aagataatag aaaaaaataa actattaaat
    42301 tttatagata aattgggaag gtagtattat gataagcaaa agagtgggga ttgttgaatc
    42361 aatagtaagt caaactgaaa ctctagatga tataagagta aatataaatg gaaaaataca
    42421 aagggcatat aattatccca aaatatctgg aactataaat ataggtgatg aagtggtttt
    42481 aaatacaaca gcagtagaac tgagcttagg tacaggtgga taccattttg taataactaa
    42541 tttaaataat atagaatcta ctttaactga aggtgggcat ataatgaaac ttagatatac
    42601 acctcttcaa ataaaagtgg attctgttga ggaacaagaa agtatttatc atgataaatt
    42661 tgcgaatttt aaaggattag aaggtctttc tgttgtagta gggactcttc atagtatgtt
    42721 aactccattt gcagcttcat ttaaaagaaa taatccaaat aaaaaattag tatatataat
    42781 gactgatgga gcatctcttc caatttattt aagtaaaaat gtggacactt taaaagaaaa
    42841 aaaattaata gatagtacga taactatagg aaatgcgttt ggtggagatt atgaatgtat
    42901 aaatatatat actgcattaa ttacagcaaa agaaatacta aaagcggatg cagtatttgt
    42961 cagtatggga cctggaattg ctgggacagg aacaaaatat ggatttacag ggattgaaca
    43021 aggctctatt cttgatgccg taaaaaaact tgaaggaaga gcaattgcaa ttccaagaat
    43081 tagttttgct gacaaacgag aaagacatca aggtatttca catcatagta tgactgtatt
    43141 taaagaaatt gtaaatgtaa atgtagacat accaataact atatatgatg atgaaaaatt
    43201 gaactttata aaagagcaaa tcaatttaaa tggtttagac aaaaagcata atataattta
    43261 tatagataat aataaaacga aagaggattt agactatttt aatctaaaag taaagagtat
    43321 gggaagaaat tatgaacaag atgaggcatt tttcaaagct gcaagcacag cagcatatta
    43381 cttaatggag gtttaagatg gtattagaag aaaaaactat tagtagtgat agggtttata
    43441 caggtaaagt aattacttta aaggttgata ctgttgaaat tccagggcaa ggatatcaaa
    43501 aaagggagtt agtagaagtt ggtggagcag taggaatagt tgcaatcaca gatgataata
    43561 aagttgtact agttaagcaa tttagaaaac ctatagaaaa gccaatcttt gaaattccag
    43621 ctggtaaact agaaaaaaat gaaagtccaa aagaatgtgc tgaaagagaa ttaaaagaag
    43681 aaactgggta tagtgcaaaa aatattaagc taatacataa atttttcact tcagcaggtt
    43741 tttctaatga gataatgttt gtatatttag caacaggtct tactccagga gagaataatt
    43801 tagatgcaga tgagttttta gatgtctatg aaattgagct tgaagaagca tataacatgg
    43861 tattaaaaaa tgacgttgaa gatgcaaaaa catctattgg attattatta gtaaaagata
    43921 tgtttaaaaa ttaatattta taaaatgaat aaccctttgt cctaaagcat attattttat
    43981 aaaggacaag ggggaattaa attgagaaga acatatagaa aaaatgattt tagagaatta
    44041 aatagacaaa taattattat aggattgttg tttatgatgt ctattgttgt tgggtcttac
    44101 ataaataaaa tattgccagg ttctagtaac aatatattaa acaatataaa tcctgctgta
    44161 gagtattata atctaaatat aagcataaaa gatacagtga tacaaaattt aaaatcagat
    44221 gcaatcttta tgggctcaat agctctatta agtctatttg tagtaacaat tccagcagtg
    44281 ttggtagctt ttgtattaaa agggatgtca attggttata caataaatag ttgtatattg
    44341 gcattaaaat ttaaaagtat aaaaatgatt ttaataatat tatttaaaaa tttgattatt
    44401 attccatgtg caatcatatt agcattaatt ag
//
LOCUS       DAGQWR010000012        44012 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.012, whole genome shotgun sequence.
ACCESSION   DAGQWR010000012 DAGQWR010000000
VERSION     DAGQWR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.012"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(72..845)
                     /locus_tag="MIW95_000580"
     CDS             complement(72..845)
                     /locus_tag="MIW95_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBY3149933.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(845..1231)
                     /locus_tag="MIW95_000581"
     CDS             complement(845..1231)
                     /locus_tag="MIW95_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY3149934.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1256..1573)
                     /locus_tag="MIW95_000582"
     CDS             complement(1256..1573)
                     /locus_tag="MIW95_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBY3149935.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVKKIFTITGLDKIFK
                     VEG"
     gene            complement(1585..1845)
                     /locus_tag="MIW95_000583"
     CDS             complement(1585..1845)
                     /locus_tag="MIW95_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBY3149936.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1845..2141)
                     /locus_tag="MIW95_000584"
     CDS             complement(1845..2141)
                     /locus_tag="MIW95_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBY3149937.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2210..4636)
                     /gene="gyrA"
                     /locus_tag="MIW95_000585"
     CDS             complement(2210..4636)
                     /gene="gyrA"
                     /locus_tag="MIW95_000585"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBY3149938.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKIGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKNEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4656..6557)
                     /gene="gyrB"
                     /locus_tag="MIW95_000586"
     CDS             complement(4656..6557)
                     /gene="gyrB"
                     /locus_tag="MIW95_000586"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBY3149939.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6558..7673)
                     /gene="recF"
                     /locus_tag="MIW95_000587"
     CDS             complement(6558..7673)
                     /gene="recF"
                     /locus_tag="MIW95_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBY3149940.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7691..7897)
                     /gene="yaaA"
                     /locus_tag="MIW95_000588"
     CDS             complement(7691..7897)
                     /gene="yaaA"
                     /locus_tag="MIW95_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBY3149941.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVKIEGTKIKVL"
     gene            complement(8034..9140)
                     /locus_tag="MIW95_000589"
     CDS             complement(8034..9140)
                     /locus_tag="MIW95_000589"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBY3149942.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9382..10704)
                     /gene="dnaA"
                     /locus_tag="MIW95_000590"
     CDS             complement(9382..10704)
                     /gene="dnaA"
                     /locus_tag="MIW95_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBY3149943.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11360..11497
                     /gene="rpmH"
                     /locus_tag="MIW95_000591"
     CDS             11360..11497
                     /gene="rpmH"
                     /locus_tag="MIW95_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBY3149944.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11575..11919
                     /gene="rnpA"
                     /locus_tag="MIW95_000592"
     CDS             11575..11919
                     /gene="rnpA"
                     /locus_tag="MIW95_000592"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBY3149945.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11951..12202
                     /gene="yidD"
                     /locus_tag="MIW95_000593"
     CDS             11951..12202
                     /gene="yidD"
                     /locus_tag="MIW95_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBY3149946.1"
                     /translation="MNKIIYILKEISRYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12234..12941
                     /locus_tag="MIW95_000594"
     CDS             12234..12941
                     /locus_tag="MIW95_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBY3149947.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12910..13539
                     /locus_tag="MIW95_000595"
     CDS             12910..13539
                     /locus_tag="MIW95_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBY3149948.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13651..15030
                     /gene="mnmE"
                     /locus_tag="MIW95_000596"
     CDS             13651..15030
                     /gene="mnmE"
                     /locus_tag="MIW95_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBY3149949.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGERALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNILDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKEIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15125..17020
                     /gene="mnmG"
                     /locus_tag="MIW95_000597"
     CDS             15125..17020
                     /gene="mnmG"
                     /locus_tag="MIW95_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBY3149950.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVDKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQVRR
                     TRGGKGE"
     gene            17013..17732
                     /gene="rsmG"
                     /locus_tag="MIW95_000598"
     CDS             17013..17732
                     /gene="rsmG"
                     /locus_tag="MIW95_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBY3149951.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGRPAKNPIK"
     gene            17907..18692
                     /gene="noc"
                     /locus_tag="MIW95_000599"
     CDS             17907..18692
                     /gene="noc"
                     /locus_tag="MIW95_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBY3149952.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18827..19600
                     /locus_tag="MIW95_000600"
     CDS             18827..19600
                     /locus_tag="MIW95_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBY3149953.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19602..20465
                     /locus_tag="MIW95_000601"
     CDS             19602..20465
                     /locus_tag="MIW95_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBY3149954.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20498..21640
                     /locus_tag="MIW95_000602"
     CDS             20498..21640
                     /locus_tag="MIW95_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBY3149955.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVSDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21774..22349)
                     /locus_tag="MIW95_000603"
     CDS             complement(21774..22349)
                     /locus_tag="MIW95_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBY3149956.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDTSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22609..23901
                     /locus_tag="MIW95_000604"
     CDS             22609..23901
                     /locus_tag="MIW95_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBY3149957.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24006..24608
                     /gene="yedF"
                     /locus_tag="MIW95_000605"
     CDS             24006..24608
                     /gene="yedF"
                     /locus_tag="MIW95_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBY3149958.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24681..24944
                     /locus_tag="MIW95_000606"
     CDS             24681..24944
                     /locus_tag="MIW95_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBY3149959.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24959..25144
                     /locus_tag="MIW95_000607"
     CDS             24959..25144
                     /locus_tag="MIW95_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBY3149960.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25247..26443
                     /locus_tag="MIW95_000608"
     CDS             25247..26443
                     /locus_tag="MIW95_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY3149961.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLEKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26657..26935
                     /locus_tag="MIW95_000609"
     CDS             26657..26935
                     /locus_tag="MIW95_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBY3149962.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            26972..27406
                     /gene="ssb"
                     /locus_tag="MIW95_000610"
     CDS             26972..27406
                     /gene="ssb"
                     /locus_tag="MIW95_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBY3149963.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27425..27652
                     /locus_tag="MIW95_000611"
     CDS             27425..27652
                     /locus_tag="MIW95_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBY3149964.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27795..28115
                     /locus_tag="MIW95_000612"
     CDS             27795..28115
                     /locus_tag="MIW95_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBY3149965.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28123..29079
                     /locus_tag="MIW95_000613"
     CDS             28123..29079
                     /locus_tag="MIW95_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBY3149966.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29090..31084
                     /locus_tag="MIW95_000614"
     CDS             29090..31084
                     /locus_tag="MIW95_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBY3149967.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31085..31534
                     /locus_tag="MIW95_000615"
     CDS             31085..31534
                     /locus_tag="MIW95_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBY3149968.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31553..32881
                     /gene="dnaB"
                     /locus_tag="MIW95_000616"
     CDS             31553..32881
                     /gene="dnaB"
                     /locus_tag="MIW95_000616"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBY3149969.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33086..33394
                     /locus_tag="MIW95_000617"
     CDS             33086..33394
                     /locus_tag="MIW95_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149970.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33476..34765
                     /locus_tag="MIW95_000618"
     CDS             33476..34765
                     /locus_tag="MIW95_000618"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBY3149971.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNKIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35091..35166
                     /locus_tag="MIW95_000619"
     tRNA            35091..35166
                     /locus_tag="MIW95_000619"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35124..35126,aa:Lys,seq:ttt)
     gene            35173..35247
                     /locus_tag="MIW95_000620"
     tRNA            35173..35247
                     /locus_tag="MIW95_000620"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35206..35208,aa:Glu,seq:ttc)
     gene            35252..35327
                     /locus_tag="MIW95_000621"
     tRNA            35252..35327
                     /locus_tag="MIW95_000621"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35285..35287,aa:Val,seq:tac)
     gene            35361..35435
                     /locus_tag="MIW95_000622"
     tRNA            35361..35435
                     /locus_tag="MIW95_000622"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35393..35395,aa:Thr,seq:tgt)
     gene            complement(35818..36810)
                     /locus_tag="MIW95_000623"
     CDS             complement(35818..36810)
                     /locus_tag="MIW95_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY3149972.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKEKLLSESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36819..37877)
                     /locus_tag="MIW95_000624"
     CDS             complement(36819..37877)
                     /locus_tag="MIW95_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBY3149973.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38002..39465
                     /locus_tag="MIW95_000625"
     CDS             38002..39465
                     /locus_tag="MIW95_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBY3149974.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39567..40364
                     /locus_tag="MIW95_000626"
     CDS             39567..40364
                     /locus_tag="MIW95_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY3149975.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40377..42479
                     /locus_tag="MIW95_000627"
     CDS             40377..42479
                     /locus_tag="MIW95_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBY3149976.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEKEI"
     gene            42547..43026
                     /gene="rlmH"
                     /locus_tag="MIW95_000628"
     CDS             42547..43026
                     /gene="rlmH"
                     /locus_tag="MIW95_000628"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBY3149977.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIVLAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43175..43486
                     /locus_tag="MIW95_000629"
     CDS             43175..43486
                     /locus_tag="MIW95_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3149978.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43534..43851
                     /locus_tag="MIW95_000630"
     CDS             43534..43851
                     /locus_tag="MIW95_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY3149979.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SKEVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
BASE COUNT        16597 a         5534 c         7009 g        14872 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata ccatctttaa aacagtaggt caatactttt
       61 tttatatttt tttataaatt tttttcatat tcttttttca gcttctctac tacctttttc
      121 tctagtctag aaacagtcat ctgtgatatc cctagttctt tagctatact agattgtgtt
      181 ttatcaaaga aaaatctatc tttaaaaatt ttaacttcta attcatttaa agtttcaata
      241 aacttattaa taaaatcttc atactctata cttccaaagt taccttcttc ttttccaatc
      301 ttatcaataa gagttatatc tttatcctct gaatcatcat tacttgatga atccaaagac
      361 attggttgat atccataaga agcctccata gcctctaaaa catcttcttc actaacgcct
      421 atataatcag ctatatcttt tacctttgga tgcttcttat tttcttgttc taatttaatc
      481 ttcgcatcac ttatcttttt gcttaattcc tgtattcttc ttgggactct aagtgtccat
      541 accttatctc taaaatactt ttttatttct cctactatag ttggtgtcgc aaagctggag
      601 aactcaaatc ctttctcaac atcatatcta tcaatagcat aaattaatgc caatgaagca
      661 acttgatata tatcttcaaa atcaacacct ttgtttatat acttcttagc taacaatctt
      721 gctaaataca gatgtctttc aataagaata tttctaatgt ctacattttt atttttatta
      781 tataaattaa atagctcttt tgtatccata tttaggtaat gtgtagcatt agctacattt
      841 ttcattaaat atcaactcct aaatatttag tcatttttat ttttgtccct tgattatgtt
      901 ctgattcaat atctacatca tccattaaag ttttaattat aaacaatcca agaccactct
      961 cttttggatt ctctaaatct ggtgtactta ttgagctaac atcatatcct tttccattat
     1021 cttgaatttc tatattaatt gcattatcta aaatattaaa tactatatta aacacatcat
     1081 ctttactgtg ttttattgca tttgtacatg cttcagatac tgctaccttt atatcttcta
     1141 tatcatctat tggaaaaccc atcttattgg caattccaga tgctgttaat cttattatac
     1201 taacataatc tggatttgta gtaatttcca tctttatagt ctcacaagcc aaagtttatc
     1261 cctccacttt aaatatttta tctagacctg ttatagtaaa aatctttttt acgttactct
     1321 taggatttac tatgtatatc tctttttcat ttatttttaa tttttttaat acacctatca
     1381 tagctcctaa gcctgttgag tctatataat ctaaatcttt aagattaatc tttacatcta
     1441 acatattttt ttctattaaa gcatgtaaat gttcctttag tttatctgct gtagaaacat
     1501 caagttctcc atcaagacaa acattccaaa atttattttg tgaatctaaa tttgaatcaa
     1561 tattcataga catgtaaaaa cctcctattt ccttttaata attttaaata tatttcctat
     1621 actcaatatt ttattaatta cataaactat atcatctatt ccctttgata ttgatgatgc
     1681 attttcaaca atatcttgta tatgtctttc attataatct acaatcttat atgccttttc
     1741 aacaacctta tttatgctgt ttagagtttt agctaaataa attgctacta taagagcaga
     1801 gcttcctact aacactgctc caatttgcca cccccatgca ttcattagtt aacatctccc
     1861 tcatcatcaa agcttttact tattactata tcttcttctg aaatctttat atcttcttca
     1921 ttattatcat cgtccactaa atttattatt ctctctttaa catcattaaa tgtttcatgt
     1981 agaacttctt ttggattttc tttgacatca ttaaacttct cttttgtttc ttttcttagt
     2041 tcagaacctt ttttaggtgc aaaaaacata ccaaatataa accctaaaat agctcctaat
     2101 aataaaaatc cacctttttt acaacttctt ttattgcaca taacaattac tcctttcaat
     2161 tttaaatttt tatctaacta cattatacaa aaagaatagg atatttatcc tattcttctt
     2221 caatatttat tttggcaatc gaaactacat tgacttcatc attcgtcttc attaatttaa
     2281 caccacttgt aactcttcca aacagtgata tttcattgac tcttattcta ataagaactc
     2341 catcagaatt tataatcatt atctcatcat cttcgttgac catatctgca cctacaattg
     2401 taccagtttt ttcagatata ttataagttt taattccttt accagctctt atttgacttc
     2461 tatactcttc tatatttgtt ctctttccaa aaccattctt acttacaact aacacatctg
     2521 tacctttact gcaaaggttc atagacacaa caaaatcttc tttacttaaa gttatacctt
     2581 ttacacccat tgctgttcta cccatatatc ttatatcatt ttcatcaaat cttatagaca
     2641 taccttcttt agttactaaa agtacttctt gttttccatc tgtaagttct actccaataa
     2701 gctcatcatc atctcttaaa cctatagcaa taagaccaga tttatttata tttttgaact
     2761 cttctctctt agtcttttta acaatacctt ttttcgttgc aagtaataaa tattcatttt
     2821 catcattacc atcaataggt attagagtag caattttctc atctgctgac aattgaagta
     2881 aattcactat agcagtacct ttagcctgtc tcttaccttc tggtatttca tatgcattta
     2941 atttaaatac tctaccttta tttgtaaaga ataatagtct actatgagta gttgtagtta
     3001 ccaagtgcct tacaaaatct tcttctctag ttgtaagtgc tgaaatacct cttccacccc
     3061 ttttttgact cttataagta tcagatggaa gcctttttat atatccaaag tgagtaagag
     3121 ttattgctat ttcttcatca cttataagat ctctcatatc tatttcgcct tcagcatgtc
     3181 ttatttctgt tcttctctca tcagagtaat tttcttttat tattgtaatt tcattcttta
     3241 ttacatttaa aagtaatctt tcatcagcta aaatttcttt taatctattt atcttcttga
     3301 ttaaatcttc atattcagct tctatcttat ctctttctaa acctgtaagt ctttgaagtc
     3361 tcatatctaa tatagcttgt gcttggattt cagttaattt gaatttttct attaaaccta
     3421 tcttagcttc ttgcccagtc tttgaagctc ttatcaagct tataacagca tctatattat
     3481 ctaaagcaat ctttaatcct tctaaaatat gtgctcttgc ttcagcttta tttagttcaa
     3541 atttagttct tcttgtaaca acatcttctt gatgtttaat ataatgatat aatatttgtt
     3601 taagatttaa aactcttggc tgaccatcta caagtgcaag cattattata ctaaaagtat
     3661 cttccatttg agaatgttta tacaaattat ttaatactat attagcatta gcatccctct
     3721 ttaattctat aacaattctc ataccatttc tattactttc gtctcttagg tctgatatac
     3781 cttctattct cttttcttta actaactctg ctattctttc aaccagttta gccttattta
     3841 cttgataagg tatttctgta actattattt gctgttttcc ttttggtagt tcttctataa
     3901 aagctctaga cctaacttta acttttcctc ttccagttct gtatgcttct gctatacttt
     3961 cttttcccat tataattgca gctgtaggga aatctggtcc ttgaacaaat tttattaaat
     4021 catctacact acactctgga ttatctatca aatatacagt tgcatcaatt acttctgcta
     4081 aattatgtgg aggtattgaa gttgccatac caacagctat accatttgag ccatttacta
     4141 ataaattagg atatctagct ggcaatactg aaggctcttt taacgactca tcaaagtttg
     4201 gtttaaagtc tacagtttcc ttttcaatat ctctcagtag ttctaatgat aatttactca
     4261 ttttagcttc tgtataacgc atagcagctg gtgaatcccc atcaacagaa ccaaagttac
     4321 catgaccatc tactaaaagt gctctagttg aaaaatcttg tgccattctt accatagcat
     4381 aataaacagc agtatctcca tgaggatggt acttacctaa aacgtcccca acaatacgag
     4441 ctgacttcct gtatggttta tctggagtta aatttaactc actcattgaa tataatattc
     4501 ttctatgaac tggctttaaa ccatctctaa catcaggaag agcacgtcca gctataacac
     4561 tcattgaata atcaatatac gattttttca tttcttccgc tatttcaata gggagtattt
     4621 tgttattttc ttccatgtat actaatcccc tctctctata tatctaaatt tctaacaaat
     4681 cttgcatttt cttctataaa ttcttttctt ggagcaacct tatcacccat aagcattgaa
     4741 aatacttcat ctgcaattgc agcatcttct acagtaactt gtaataaagt tcttgtctca
     4801 ggattcatag ttgtttccca taattgctct gcattcatct ctccaagacc tttgtacctt
     4861 tgaagttcaa caccatttct tccaatttca tctaataaga tatttaactc tttatctgag
     4921 taaacataat gttccttctt ttgctttgtc actttatata aaggtggctg tgctgcataa
     4981 acatacccct catctataag aggtctcata tatctaaaga agaatgttaa taataaagtt
     5041 cttatatggg ctccatctac atcagcatcg gtcataatta taattttatg atacctagcc
     5101 ttatctatat caaaatcttc tccaatacca caaccataag ctgttatcat attttttatt
     5161 tcatctgaag atagtatcct atctagtctt gatttctcaa cattaagtat tttccctctt
     5221 aatggaagta tagcttgact atttctatct ctaccttgtt tagctgaacc tcccgctgaa
     5281 tccccttcga ctaagaatat ttcactttta gatggatctt tttctgcaca atctgcaagt
     5341 tttccaggta aagatgtact ttctaataca ctttttctcc ttgttaattc tcttgctttt
     5401 tttgcagctt ctctagctct ttgtgctctt aaagctttat caactatgat tcttgctgtt
     5461 gatgggtttt cttctaggaa agaccccagt tcatcaactg ttacactatc aactatacct
     5521 ctcataaagc tattacctag ctttgtttta gtttgacctt caaattgagg ttctggtaat
     5581 ttaactgata ctactgctgt aagaccttct cttatgtctt caccaagtag gttaacatca
     5641 ttttccttta aaaatttatt tctcttagca taatcattta cagttttagt aagagctgct
     5701 ttaaatccac ttaaatgaga accaccttcg tgtgtattta tattgtttgc aaaagaatat
     5761 atgttctctg tataaccatc tgtatattgc atagctagtt caacaaaaca atcatcaacc
     5821 tttttatcta tataaactat gtcatcatgt ataccagttc tagatttatt taaatactta
     5881 atatattcta ccaaaccacc tgtatagtga aattcttttc ttttttcttg tccttctctt
     5941 ttatcttcaa atactatttt tattccttta ttcaagaaag aaagttctct aagcctatac
     6001 tccaatgttt catatttaaa ctctatttca tcaaatattg tctcatctgg cataaattga
     6061 atcattgttc ctttatcttg agattctcca actacttcaa gcttagatgt tggtaatcct
     6121 ttctcatatg tctgcttata tatctttccg tttaaataaa cctctgctac catccatttt
     6181 gacaatgcat taactacaga aacaccaact ccatgaagac ctccagatac tttatatcct
     6241 ccgcctccaa actttcctcc tgcatgaaga ttagttaata ctgtttctaa agttgattta
     6301 ccagttttag gatgtatttc aacaggtata cctcttccgt tatcttttac taaaacactg
     6361 ccatcttcat taatagatac atatatgtct gaacaatatc cttgtaaagc ctcatctata
     6421 ctattatcta caacttcata aactaagtga tgtaaacctc tagggcttgt actaccaata
     6481 tacatacctg gtctttttct aacggcttct aatccctcta atacttgtat ctgacttgca
     6541 ccgtattctt gtttcattta atttcctcca ttctctatgc taataacatc gccattttca
     6601 atattaaata ttgtaacatt tttttcatca aatatttttt tatgtgaaac ttcagcactt
     6661 gttataaaca tttgaacatt gcttaaatta tttactaaaa gtttttgtct tgcttcatct
     6721 aactcactaa aaacatcatc taaaattaat actggatatt cttcaacttc attttttatt
     6781 aattctatct ctgataactt taatgatatt gaagcagttc tttgttgacc ttgtgaacca
     6841 aaaagtcttg catctaaatc atttatgaat atatttaagt catctttgtg tataccatat
     6901 ctggtagtct tagattctat atcattaggt ctattcgatg acaacttggc taaaaattta
     6961 ttatatactg tatctatagt atcttcatca gttatattta tttgattttt atatcgaatt
     7021 gataatcttt caactccatt agtcaaattc atatgcatat tttcagatat attagctatc
     7081 ttttttataa agtctctacg caaaatataa atataacttc catactttgc taacgtatca
     7141 tcatatacat ctaataaagc ttcatcaaca tgtatatttt taagcaccct actcctttga
     7201 gaaagtgttt tattataatt ggtaagatat ttataatatt taggtataat ctgactaatc
     7261 tctttgtcta taaatgccct tctttcttta ggaccctctt taaccaacct taaatcttca
     7321 ggagaaaata taacaacatt taagttgcca agtagttctt gtatcttttg taaaggaact
     7381 ttatttattc ttatcccctt tttatctttt ccaataatta gctctattag actatattta
     7441 ttatattttg aaaaacttcc tccaatgtat aagttttcac tattaaatct aaccatttct
     7501 ttatctttgt tagtcctaaa tgattttcca aaagaaagca tataaataga ttctactata
     7561 tttgtctttc cttgaccatt tttaccaaca agtagattta ctttcccatt aaattccaga
     7621 tgcaactttt tataatttct aaaattaaca agttgcaaac ttttaagttt cacaaaaagt
     7681 tctccttttt ttacaatact tttattttag ttccttctat tttaactata tctcctgatt
     7741 ttatcttttt tcctcttctt aattctattt catcatttac tgtcactaaa ccttcttgaa
     7801 ttaaaaattt tgcatgacct cctgttgatg caatctctgc taatttaaga aattgatcca
     7861 actttatata ttctgattct atagttattt cagtcataat agttcctcct tgtcactatt
     7921 ttaaacaaat tcctattgat acactaaaag tatattatac cataaatatg cttataaaag
     7981 gagttgtcta agattagaaa aattttctac aaaagacaac tccttcatgt ctgctatata
     8041 tttgatgata ttcttactgg aagcagtaag taaatataat taacatcatc tgttggttta
     8101 attatacaag gatttacatt tgtagtaaat tctataaata tttcctcatt atctatattt
     8161 ttcaaacctt cgataaaata tctagaattg aaagctatat ctaggtaatc tccatctaaa
     8221 tctatttcaa cttcttcata cacatttcct ttttctgtgt tagaagtaat tgccattact
     8281 ttatctctta tagataactt tataagatta tttttttctg attgagacaa taaagacgct
     8341 ctctctatac tatttaaaag ttccttagtg tttaatttaa ctctgctgtt atgttctctt
     8401 ggcaataatt ttttataatc aataaaatct ccttcaagca atcttgtaat aattttcgta
     8461 tcattaatta taaaaatagc atttttttcg ttaaacccaa ctttaacatt atcctctcca
     8521 gaaagcaaac tatttacatc tataagtgtc tttccaggaa ttatgacttt tatattttca
     8581 gttaaactat caactgaaca actttttaca gctaatctat atccatcaat tgctactaaa
     8641 tttaagtttc tatctacaat ttctaaaagt tctcccatta aaacaggttt tgtttggtcc
     8701 tgtgagatag caaatacagt ttgtttaatc atatttttta gaatttcttg aggtatgcta
     8761 tataaatctt cttcatttaa ttctggtaat ttaggaaatt cttttgctgc ataaccttta
     8821 attttaaatc ttgaatttac acaatttatg taaatattat tttctgaatc agtttctatc
     8881 tcaacaaagg aatctggtaa ttttctaatt atatctccaa atagccttgc atctacaaca
     8941 acatcacctt tttctatgat ttctgcttgt acataagttt ctattcctat ttcagcatca
     9001 tatccagtaa gttttaattg accttcttct gtagatataa gtataccttt taataattct
     9061 attgttgttt ttccattaat agctttttga gcaattccaa ttctatttgc taggattttt
     9121 tgattacaaa ttattttcaa tgtggataac ctcctctggc tttttatata taaaaataag
     9181 ttaaaatcat agtagtaata gtagtaggtg ttgttgataa tgttaataag tatgtataac
     9241 attataaata caaagatatt aacatgtgta taagatgtta atatctttgt tattttagac
     9301 ttgataattt gttaataatt tttgttcaaa aaaagtatta acaggttatt caagagatat
     9361 aaacatacaa ttgtgtataa attatccctt caaatctgat ataattttgt ctattttagt
     9421 cttaatttct tcactttctt ctatatcctt agatactttg tcatgagcat gaattactgt
     9481 tgtatggtct ctacccccaa actcttcacc tattttagga agggataaat cagtaagctc
     9541 gcgcgttaaa tacatagcta tctgtcttgg ataagctatt gaacgtgttc tttttttaga
     9601 attaaaatct tccatcttta aattgaatac agaagatact ttttctttta ttctaagaac
     9661 atttatttct tcatttttag aagttgttat aatatctttt aaagcttctg tagcaagatc
     9721 aaaagatatt accctattgc taagagaaga ataagcaacc actctagtta atgcaccttc
     9781 tagttccctt atgtttgact taatattttt tgctatatat gacataacct catttggcac
     9841 atctatacgt tctagttgag ccttttttct aagtatagca attcttgtct caaagtcagg
     9901 tgcttgaata tcagttataa gtcccatttc aaatctcgat cttagcctat cttctagtgt
     9961 aggtatgtct tttggcggtc tatcactaga tataattatt tgtttgtttg cttcatgaag
    10021 agtattgaat gtatggaaaa actcttcttg agttctttct tttcctgcta taaattgaat
    10081 atcgtcgatt agtaatacat ctacgtttct atatttattc ctaaattctt cgtttttatc
    10141 gtcttttata gagtttataa gttcattagt aaatttttct gaagaaacgt aaactacttt
    10201 tgagtctttt ttttgactaa caatatgatg acctatagca tgcattaggt gagtcttacc
    10261 taatccaact cctccataca aaaaaagtgg gttataagct ttagctggag attctgcaac
    10321 tgcaacgcat gcagcatgtg caaatctatt gctattacca ataacgaatg tatcaaatgt
    10381 gtattttgga tttagattgg ggtaaagaac tctataattt aaatctgtgc tatcagaatt
    10441 taaatcagca acctcttttt cgctcaatac aaatttaata ttatattttt ttaatgacag
    10501 ttgactaata gcatcttcaa ttaaatgtaa atatctattc tctaagatat ctttattaaa
    10561 atcactagga gccagtaaga ttaaatcatt aagatgtatt tttaatggta cgatgttttt
    10621 aaaaaaggta ttaaaactta ctgaagttaa gtcacctttt attaattgta gggttttgtc
    10681 ccataaagaa actatatcca taatataaac ctccgaacag aataattaat attttccaca
    10741 aagttattaa cagaaaagca aaaatatata taaattttac agaataacta aatttatgga
    10801 taatggtgtt gataaattaa taatagtata aattattaaa aaaatatgaa aagatataaa
    10861 caatatgtag ataaagttat aaataacata aacatacaat aaatggtgat tataactaga
    10921 gtattgtcag taatcttaaa agttatacac aaaagtatcc acaagctgtg aataatttct
    10981 aataagttga taacatttat gcacattatc aacaagtata tttataatat caaaatatat
    11041 gtcaaacttc aatgatttgt aactaattta tccacaatat taactaattg ttgataaaaa
    11101 tataaacatg ttaaaattac tttatttgtg tatataattg aaaaaaataa tttagaataa
    11161 aattaaatag atttttgcta gtattttcaa taaaaaaaat acataatata tatatggtag
    11221 attttgtgat ataagcgtcc caaaaactcg ttgacaacag tagtaaaagt atttataatt
    11281 atatgtgtta tgtttacttt gagggtaaag ttattatttt tataaacgga aaataaaatc
    11341 tgaaaggcgg tgcaaaataa tgagtaaaag aacttatcaa ccaaaaaaga gacaaagaag
    11401 taaagaacat ggtttcagaa aaagaatgaa gacgtctaac ggaagaaacg tgttaaagag
    11461 aagaagagct aaaggaagaa atagattaac tcattaatta caaaagggcc gctgcaaagg
    11521 tggccttttt gtgtaaataa ttacaattca tagctgtgtt tatggaggtt aactatggac
    11581 tttaatagga ctaaagggtt gaaaaaagac tctgatttta gaaaagtata taaacacggc
    11641 aaatcttttg caaacaaata tttagtaata tatatactta aaaataaatc agattatagt
    11701 agagtaggta tttctgtttc aaaaaaagta ggaaaagcta ttactaggaa tagagtaaga
    11761 agattaataa aagaagccta cagattaaat attgatgaaa aaattaagcc tggatatgat
    11821 atagtattta tagcaagggt atctagtaag gatgctactt ttaaagacat agacaagtct
    11881 ataaaaaatt tagttaaaag aacggatatt tctatttaat tgtatgctat aaatatggag
    11941 gttaatgact ttgaataaaa taatttatat attaaaggaa ataagtaggt atttatcgaa
    12001 tttatgtatt tatttagtga gattttatca aaagtatata tctcccttaa agggacctac
    12061 ctgtagattt tatccaactt gctcacaata ttcaatagag gcgtttaaaa aatatggatt
    12121 tgtaaaaggt atgtatttaa cgataagaag acttttaaaa tgtcatcctt ttcatccagg
    12181 aggttacgac cctctaaaat aaaagagagt aactattagg aggtataaaa aaattgaata
    12241 taataggaaa tttcttagga ttgattctta aggtaatatt tgaatttgtt aatcattatg
    12301 gtatttctat aatacttttt acgatacttg taaagattat tttattacca ttaacaatta
    12361 agcagactaa gtcaacaaag gcaatgcaag atatacaacc tcgtataaaa gagatacaag
    12421 aaaaatataa aaataagcca gaaaaacaaa atgaagaaat tgttaagtta tatggagaag
    12481 ctaaaattaa tccactatct ggttgtttac ctttattaat acagtttcct atattaatag
    12541 gattatttag cgttttgaga gaaccagttg ctcatggcgt ttttgcaaac aaagctgcat
    12601 ttttagcagc tgacaatggg tttttatgga taaaaagctt aacatcacca gattatgtat
    12661 tagcagtatt ttcaggagca agtgcatatg tcatgcaaaa agtaatgaca cccaaagatc
    12721 agttacaagg ctctatgaaa gtaatgacat atgtaatggc aggtatgtct ttctattggg
    12781 gatttatatt cccagcagga cttacattgt actggacagt aagtaactta ttctcaatag
    12841 ctcaatatta cttaataatg aatcctttaa aagctaaact ggctgctaac tctaaggagg
    12901 aaaaagttaa tgagaaaaag cctaaaatta ataaaaagta agagtaaaga ggaagctata
    12961 agtaaagcta tagcagagtt gaatttaaaa gcagaggata tagaagtaga aatacttgaa
    13021 aatccaagta aagggttttt aggttttata ggtgctaaag atggtactta tgaaattttt
    13081 gttatcgaga gggaagtaga tgtagctaaa aattttatag aggtaatgtt gaaaaatgca
    13141 aatgttgatg ccaaagtcaa tgtatctcaa aaagataatt taatcatggt tgatattgaa
    13201 ggcaaagaag ctgcaagttt aattggtaga agaggggaaa ctttagattc tattcaattt
    13261 ttgactggat tagctcttaa taaaataaat aaagattctc atacaagagt acttgtagat
    13321 atagaaaatt atagatcaaa acgagaagaa tctttaataa gatatgctaa taaagttgct
    13381 agagaagtag cgaaaacaag aaaaactaaa aaattagatt atatgaatcc atatgagaga
    13441 agaataatac attcagctct tcaaaatgac aaatatgtaa ttacatatag tgaaggaacc
    13501 gatccatata gaagattagt aattgagtgt aaaagataaa gtcaacctcc taaacattag
    13561 ggggttttat tttttaatat tattaaatca ttaatgattg gttaaatgta aacctatatg
    13621 taaaaaatta tgaattaaga ggtgataaat ttgtttatag atgatacaat tgcagcaata
    13681 gctacagcac ctggagaagg tggtataggt atacttagaa taagtggaga aagagctttg
    13741 aaggttgcag aagaaatctt taaatctatg tcgggaaaga gtattgaaga atacaataag
    13801 agaacattaa tatatggaaa tatattggat aatgaaaata taattgatga agttttattg
    13861 gcatatatga aaggaccaaa ctcctataca ggagaagatg ttatagaaat taattgtcat
    13921 ggtggattta tttcggtaaa gaagatatta gaattaatac tatcaaaaga tgttagatta
    13981 gcagaggctg gtgagtttac taaaagagca ttcttaaatg gaagaataga tttatctcaa
    14041 gcagaggctg taattgatgt tataaaagcc aaaactgata tagctcatga agttgctcaa
    14101 aatcaattag agggttcttt atcaaagaag ataagagaat tgagagataa agtcactgag
    14161 atattagctc atgtagaagt tgctatagat tatcctgagg aagatattga gcatataaca
    14221 tatcaaactc ttaaagaaaa aactgatgaa cttaaaaaag agattaaaaa attatatgat
    14281 acagctgaaa gtggaaaaat acttagagaa ggcttaaaga cggtaattgt tgggaaacca
    14341 aatgtaggta aatcttcact acttaattct attttaggag agaatagagc aatagttact
    14401 gatataccag gaactactag agatgttata gaagagtttg taaacataaa aggtatacct
    14461 ttaaaaattg ttgacacagc aggtataagg gatactgatg atatcgttga aaaaataggt
    14521 gttgaaaagt ctaaagaatc atttactagt gcagatttga tagtaatggt tttagatgca
    14581 tctagaaagt tgtcagagga agatatagag atattagaaa aacttaagga taaacaaaca
    14641 atagtgctgt taaataagaa tgatttgaaa caagagattg aagaagaaaa aatacttaaa
    14701 tatgtagaaa ataatagtat aataaaaata tcagctttac aacaagaagg aatagaagaa
    14761 ttgcaagata aaatagagtc tatggtatat aaaggtagta tcaaaaataa ttctagttta
    14821 gtagtaacaa attcaagaca taaagatgca cttagtaaag catataaatc tgcaacagat
    14881 gctttaattg ctttggaaca aagtatgcca tttgactttg tagaagtcga tttaaaaaat
    14941 atatgggact atcttggata tattaatggt gatacagtaa cagaggattt actagataat
    15001 atattccata atttctgtat tggtaagtaa tgtacaaaag taaatttgtt tttattatag
    15061 agatttttaa aaaatttgaa gtgtttctca tgaaacatta attttgaaaa gaaaggaatt
    15121 ataaatgata aagtttgaag caggaaaata tgatgttata gtagtcggag caggacatgc
    15181 tggatgtgaa gctgctttgg ctactgcaag aatgggatat aaaactttga taataacaat
    15241 gtctttggat tccatagcat taatgccttg taatccatca attggaggga ctggtaaagg
    15301 tcaattagtt aaagaaatag atgccttagg tggtcagatg ggattaaata tagacaagac
    15361 atatatacaa agtagaatgt taaatactgc caaaggtcca gcggtacact ctttaagagc
    15421 acaagcagat aaatttaaat atcatgaaga aatgaaaaag acattagaag atgaaccaaa
    15481 tcttgatata gctatggatg aagttgtaga aatacttcat gaaggaaatg ttgtaatagg
    15541 agttggaact aaactaggat gttcatttaa atctaaagct gtaatattgg ccactggagt
    15601 ttatctgaac tcaaagatat atatgggtga agttgcattt tatgaaggtc ctaatgcatt
    15661 ggggtatgca aaatatctta cagatagctt agttgagctt ggtcttagaa tgagaagatt
    15721 taagacggga actccagcaa gagttcatag agatagtata gacttctctg taatgtcttt
    15781 acaagagggc gatgaaaaag taactccatt ctcatttatg aatgaaaata tagaaaaaaa
    15841 acaagaacct tgttatttaa caagaactac agaagaaact caaaaagtta tactggataa
    15901 tttaaaaagg tctgctatgt atagtggagt aatagaaagt actggtccaa gatactgtcc
    15961 ttctatagaa gataaagtag taagatttag tgataaaacc tcacatcaat tatttataga
    16021 accagaaggg ttaaatacta aagaaatgta tatacaagga atttcaacta gtttaccttt
    16081 tgaagttcaa ttagatatgt acaaaactat aaagggactt gaaaattgta agataatgag
    16141 acctgcatat gctattgaat atgattgtgt tgacccaact caactaaaaa taagtttgga
    16201 aataaagggt gtcgagaact tatttagtgc agggcaattt aatggaactt ctggatatga
    16261 ggaagcagca gctcaagggc ttatggctgg tataaatgca gttagaaaga tagaaggaaa
    16321 agagccgttt gtattagacc gttcagaagc ttatataggt gtcttattag atgaccttgt
    16381 tactaaagga actaacgagc cttacagaat gatgacatca agagcagaat atcgactata
    16441 tttaagacaa gataatgcag atatgagatt aactcaaaaa ggttatgata taggattagt
    16501 taaaaaagat agatatgaaa ggttcctaaa taaaaaggct gctgtagata aagaatttga
    16561 aagattaaaa aatgaaagag ttacaccaaa agaagtaaat agtttgttag aagaaaaagg
    16621 agcaacacca ataaaggttg gaatatctct atatgaattt ttaaagagac cagaggttac
    16681 ttacgaattg cttgaagaat tagggaaggg cgctggagaa gatgtttcaa gagaagttaa
    16741 agaacagtgt gtaataataa ctaaatatga aggttatata gagaaacaat tgaaacaaat
    16801 agaccaattt aaaaaacttg agaataaaaa gctggatgag aaaataaatt attcatctat
    16861 agaaggtctt agattagagg ctagacagaa attagatgat ataaaaccta tttcaatagg
    16921 gcaagcatct cgtatatcgg gtgtttctcc agctgatata tcagttttac ttatatacct
    16981 agaacaagtc agaagaacta gaggtggcaa aggtgaataa tatagaactt ctgaaaaacg
    17041 ggattgaagg ttttaatata gatacgaatg acagtatgtt agaaaaattt aaaatctata
    17101 gagagatatt agttgaatgg aataaaaaga tgaatcttac tggaatagaa gatgaaaaag
    17161 aagtgtatat aaaacatttt ttagactcta tttcagctgt aaagaatgga tatataaaaa
    17221 atgggatgtc tataatagat gttggtacag gtgctggttt tcctggaata ccattgaaaa
    17281 tatgtttaga tagcttagaa cttactttgt tagattcgtt aaataaaaga ataaactttt
    17341 tagaggaagt tagtagagta ctagagttag ataatattac atttatacat ggaagagctg
    17401 aagattttgg taaaaatgaa gactatagag aaaaatatga tgttgctacg gcaagagctg
    17461 ttgctggatt accgattctt atggaatttt gtgtaccatt tgtaaaagtt ggagggtatt
    17521 ttatttgctt aaaaggtccg aatgctaatt tagagctaga agaatcacaa aaggctattg
    17581 atgttttagg attggagtat attgaaaaaa tagatgtaga gttaccagag gtagatttaa
    17641 atcataatat attagttttt aaaaaggtga tggaaacacc agttaagtac cctagaaaag
    17701 ctggtagacc agctaaaaat cctattaagt aatgaaaaaa gtaatgaaaa aattattatt
    17761 ggtaagctta attatttctt aggaaataat taagcttacc gtaaaaaaac aaaaataaag
    17821 gaattattaa agtagatgca gaaataaatt tgtatgtaca tatagagaac aaaaataatt
    17881 tatatctaaa ataagagagg tatgttatgg aagataaaag agttatggag atacctatag
    17941 aagatgtagt tccaaatcca tatcaaccaa gaaaaatatt ttcacaagtt tcattggagg
    18001 aattgagcaa ttctataaag gtatatggta taattcaacc tataacagtt agagcaaaag
    18061 atggcaaata tgaactaatt gcaggagaaa gaagattaag agcagctaaa ttagcagagc
    18121 ttaaaaccat accagcaatt ataaataata tgaatgatga atcttctgct gttctagcgt
    18181 tattagaaaa tttgcaacga gaagacttaa atttcattga agaagcaatt ggttatgaaa
    18241 acttaataaa agaacatgct tttactcaac agcaattagc agaaaaattg ggcaaaaatc
    18301 aatctactgt agcaaataag cttagaatat taaagcttcc aaacgatata aaaatgaagc
    18361 taatagagaa taatttaaca gaaagacatg ctagagcatt tcttaaacta ccaagtgaag
    18421 atttaatgca aagtgtgtta gacaaagtca taaaaaatga attgactgtt aaaaaaacag
    18481 aaaaattgat acaagatgta ttagaagaaa ctaaagttca agaagagcct gataaaaaac
    18541 agaatataaa gggtgctatg agtataagaa tttacataaa tactataaag caggcctttg
    18601 atgctatatc aaatacagga attgatgcta agtataatga aatagataag ggagattata
    18661 tggaagttgt tgtgaaaatc cctaaaaaat agagattact gcttaccagt aatctttttt
    18721 tgtaatataa gccttgtaat ataaattgat ttatagacat tatttatact taaaaaacac
    18781 ctattcaaca tttaaaatta atttaattct taaagggggg aacatcatgg gcaaagttat
    18841 agctgtattc aatcaaaagg gtggagtcgg gaaaactaca acaaatgtaa atttaagtgc
    18901 tagcttagga actttgggaa agaaaatatt agtattagat ttagacccac aaggaaatac
    18961 aactagtgga tatggtatta ataaaaacga agtagaaaat acaatctatg aaattatgtt
    19021 agatgggtta catattaaag aagcaataat aagtactgaa tttgagaatg tagatgtagt
    19081 tccatcagct actgaactat caggtgcaga gatagagctt acatctaaaa ctaatagaga
    19141 gtatatttta aaaaattcca taaaagcggt aatagatgaa tatgattata tatttttaga
    19201 ttgtccaccc tcactgggga tgcttacaat aaattgttta actgctgtag atagtgtttt
    19261 aattccaata caatgtgaat attatgcttt agaaggtgtt agtcaattaa tggagactat
    19321 aaaattagta aaatcaagat taaatgctga tatagaaatt caaggtgttg ttttaagtat
    19381 gtttgatgga agagctaatt tatcaataca agtagttgaa gaagttaaga aatactttaa
    19441 aggtagtgta tatacaactt taattccaag aaatgtaaga cttgcagaag ctccaagtca
    19501 tggaaaacct gtaatttatt atgataaaag atgtagaggg tctgtagctt atttggagct
    19561 agcagaagaa tttatagatt tagaagagga ggaatggtag tatggagaat aagtctaaaa
    19621 gaagtaatag attaggtaga ggtcttagtg cattgatacc agaaattaaa ggagaaactt
    19681 cagaaaaaga aatagtcaac atagatatag acaaaatata tccaaacgaa gttcaaccta
    19741 gaaagcaatt tgatgaagaa aagataaaag tattgtcgga ctctataaaa aattatggtg
    19801 ttttacagcc aatagtggtt aaaatggatg aaaataataa atatatgata atagctggag
    19861 aaagaagatt tagagcatca aaacttgcaa ataaaaatca aatacctgct ataattaaag
    19921 atatagatat gaaagatata atggagatag ccttaattga aaacttacag agggaagatt
    19981 taaattcaat agaagaggct ctagcatata agagtttaat agagcattac aatgtaactc
    20041 aggaagaaat atcagaagct gttgggaaga gtagacctca tataactaat acactaagac
    20101 ttttaaatct tggacaagat gttatagaaa tgatagacag tggtagaatt acagcaggcc
    20161 atggtaaggc tttattgaga attgcagata aagatttgca acttcaaata gcgaaaaaga
    20221 tagaagaaga agaactttct gtaagagaag tcgaaaatat agctaaaaaa atatctgaaa
    20281 ataaacaaga agaacctaaa aaatctaaac ctaaggatgt atttatttta gatgttgaag
    20341 ataaacttag aaatatattt ggaacaaagg tgaatatttc taaaggaaag aagaaaggta
    20401 aaatagagat agaatactat aatgatgatg atttaaatag tattgtatca atgcttcttg
    20461 aatagaacaa aaatataaaa cagagaggaa gattgttatg atttatttag ataatgctgc
    20521 aactacatat ccaaaaccag aaagagtgta taatgctgtt ttagattgta tgaaaaatta
    20581 ttgtgcaaat ccaggtagag caggccataa acttgctatg agagctgcta gggagatata
    20641 tgatacaaga gagaatatag ctaagttgtt caatgtaagt aatcctatga atatagtatt
    20701 tacatctaat gcaacagatt ctttaaattt agcgataaaa ggtgtattac aagaaggaga
    20761 ccatgtaata acaacgtcta tggagcataa ttcagtaata agacctataa aagcattgga
    20821 aaaaaggggt attgaaaata ctgtagtaaa atgcgattat gagggatttt tagattatga
    20881 agatttagaa aaatcaataa agagtaatac taaactgatt gtaacaacac acgcatctaa
    20941 tgtatgtgga actttaatag atataaaaaa agttggagaa atagctaaaa aacataatat
    21001 actattttta gtagatgcat cacaaacagc aggtgtttat gatatagacg taaatgaatg
    21061 taatatagat atgttggcaa tgccaggtca taaatgctta tttgggcctc aaggaacagg
    21121 tatattatat gtaagagaag ggttgaactt aaatatatta aaagaaggtg gaacaggtag
    21181 taaatcagaa gaaatagttc aaccagagtt attcccagat aagtatgaat caggaactca
    21241 taatactcca ggtatagcag gtcttaacca aggtatacta tttatatttg aaagaggaat
    21301 caacaatata agacaacatg aagaagaatt atgtcaatat atgatagaca aactagagga
    21361 agtatcagat ataaagatat atggaccaaa ggatagtaaa aaaagagcat cagtaatcgc
    21421 tctaaatata ggagatatgg attctggaga ggttactttc ttattagata gtgattataa
    21481 tatagcaact agatcaggaa tacattgttc accactagca cataccactt taggtacttt
    21541 aaaacaaggt gcagtaaggt tcagtatagg atatttcaac acaaaagatg agatagataa
    21601 agcagtggaa gctttgaaaa aaatttcaaa aaataaatag catgagattt aataatatag
    21661 aaataaaaaa aggggatatt tcaaaataaa aatttgaatc atctcctttt ttgtacaaaa
    21721 aagaaaaggg gctcctaaga acctctaatc tatttgataa ttgtttcaag aaattacttt
    21781 aagtaatctc cttcttcaag aactatatga ccactttcgt aattagaacc atcaacagta
    21841 atttttattt gttcaacatt catatttttt atgtaagtac gagcaactga atcaagcatt
    21901 aaagtttctg catcactacc taagtttgac tttgtaaagt cagagctaac atctaatata
    21961 ccagttttta caccatcaat gtcttttgta gtaaatgaat ttaattttgc agattctgga
    22021 acaactttaa gtttttgaag ttcttcaaat aaagtgttct catcaatttt atttaaatca
    22081 actttatttt caattaaatc tgtcttatct acatcaaaag agtaaatagt agctgattga
    22141 gttttagttt cttcttttgg agtaggttta gctttgttat taggaacctc tttttcagta
    22201 ttgttttctt cttttggttg ttcaacattt gtattgctct tagtttcttt tttagatgta
    22261 tcacttccat tttgagcatt agtacaacca actgcgaata tagatataga taatatactt
    22321 aacactaaca ttattttctt atttttcata aaaatttctc ctctcttaaa aactattctt
    22381 atatataatt atagtcgtta tgagcaaata tatgaataca aaatagtaac aaatttaaaa
    22441 aatgatttat ttaaattaat aaaactcaaa ataaactaaa ttttagtcat ttttatggga
    22501 aatcatttaa tgtacagtga aaatattgta aaataaaagt agtgttttta aaataaatat
    22561 aagttataat tatattataa aaaaatgtct tcaaaaggtg ggaatagtat ggaaattttg
    22621 agcttaggag aaaagattaa aaaattaaga aaagaaaaaa acatgacatt aaaagaatta
    22681 gctggagata ggataacagc agcacaaata agccatatag agagggataa atctcatact
    22741 agttatgaac tgttggaata tctaggcgat aagctagatg taagtgtaga atacttatta
    22801 gagaccaagg aaatgcaatc aaaaaagata actgataatt taatactaca gagcgaaatt
    22861 tatataaaag aaaatgaatt aaacaaggct gaggatcaaa taaaagaagt tttagatata
    22921 tgtaagagat acgatttaat ggagaactat ggcagatgta attttttaat ggctaatatt
    22981 aatttgaaaa aatctaatta taatgatgct atagaaaact ttgaaaaggc attgtacttc
    23041 tttataaaaa atagtgatag tgaaaatata cttaagtgtt atttaaacat agggaaaata
    23101 tatatgcagg aagactttta taaaggtgct ataagccact ttaattttgc agaggaaatt
    23161 ttgtcagaga ataaatttga ggatgttaac atacacaagg aactatatag taagatggca
    23221 tattgttaca taaaactaga taatccaaat atgtcattga agtacataga aaagataaat
    23281 gaaatagata atagaaatga tagaaaagaa gatgtcgata ttttggtgtt aaaagcaaat
    23341 aatatgcttg agattggaaa atatgaagaa tcaagagatt actttaaaaa agcactaaaa
    23401 atacttgaga gtgaggataa taaaactgga ttagcaaatg tgtatttaac aatttgtgat
    23461 gtttacagaa aaataggtga aacagataga gtacttgaat attcacaaaa ggtatatgac
    23521 ataaagaaaa atgatgaaga tgaatacatg atgtcaggat tatttaagat aatagaagca
    23581 tatatagata aagaagatta tgatttagct agaaaatatt gtaagatagc cttagcttca
    23641 tcaataaaaa ataaaaataa attcaatgaa tataaagcat tgagattcta tgctaatatg
    23701 tataaaaatc agaatgaagt aactcttgca attgaatatc tgattaaatg cataaaaata
    23761 gtatcagatt taggcaacaa tagaatattg gccaatttat atatagactt aggtcaatta
    23821 tattcaaata tatcaaaaga aaaagagtta gaatattatc aaaaaggtgt atttatgtac
    23881 aaaaatttag aaataatgta gggtaaataa acctataagg gtatagaaac aatctataac
    23941 aagcaagata atatctatga taaaatataa attgtatgaa tatttttaag gagatgaatc
    24001 agacaatgag tataaaaata gatgcgagag ggcttgcttg tcctaaacca gttataaata
    24061 caaaaagaga attggataac ctagaagaag gtgttgtagt tacaatagtt gataatgaaa
    24121 cagctaagga gaatatatta aaacttgcta aatctttaag ttgtgaagca aatattgttg
    24181 acgaaaaaga gaattttata tctatagaaa ttagaaaagg taaaaatata ctagataaac
    24241 aaaatgttgt agataataaa gaaagtgaat tagacaatac atgtatattt atatcttcag
    24301 ataagatggg acttggaaat gacgaattag gaaaagtgtt aataaaagga tttatatata
    24361 cattaacaga atctaaacct tatcctaagt atgtactttt agttaatgga ggagtaaaat
    24421 taagtgctga aaatgaagaa accatagaaa atctaaaaat acttgagaat atgggtgtag
    24481 aagttttatc ttgtggtact tgtttagatt attataattt aaaagacaaa ttacaagtag
    24541 gtacagttac aaatatgtat actatagttg agacattaaa gaatgcatca aataccattt
    24601 ctatataatg tatgtttgtt taataatata aaactaataa taaagattaa ctcaaataga
    24661 tagtcagaaa ggattaagta atgaatcaaa tgtatatagt gtcatttaat tcgacacatc
    24721 atgcgataag atcagaaaag ttatttggtg aaaatagtct aaaagtaatg gctcttccaa
    24781 caccaagaga aataacagca agttgtggaa tatcaattaa attttctttt gaagatatgg
    24841 aaaagataaa aactattttg gttgaaaata atgtagatat aaaaggaatt tattgtataa
    24901 gtaaattaga aaatggttca aaagaagtag agaagttaga ttaggaggct aattatttat
    24961 gccaatggat ttaaaaatag gtgatattgt tgaattaaaa aagcaacatg catgtgggtg
    25021 taaagagttt gaaatcataa gaacgggaat ggatataaag ataaaatgta ctaaatgttc
    25081 aaggctaata atgcttgata gagagacctt agaaaagaga gtaaaaaagc taataaaaaa
    25141 gtgataaaaa tttaacactt aaaaaatgtc aaataccgta tattaatatc caaaattttg
    25201 gtataataag aattgaagaa aacattttca tgggggtgct ttaattatgg cagttaaata
    25261 tgcaaaaaga atgcaaggat tacaaggatc agaaatacgt gagcttttaa aacttactca
    25321 acaaccacaa ataatatctt ttgctggtgg aatgccggct ccagagttat ttccagttga
    25381 ggaaatgaaa aaagtatcag ttgcagtgct tgaagaaaat ggaagatcag ctatgcaata
    25441 tactacaaca gaaggatatg aaccattaag agaaaaaata gcagctagaa tgaatgataa
    25501 aaacaaaact aatgtaaaca aagatgatat attagttaca agtggttctc aacaaggtct
    25561 agattttgca ggaaaagtat ttattgatga gggtgatgta attttatgtg aaagtccatc
    25621 ttacatagga gctataaacg catttaaatc ttatcaacct aaattcatag atgttccaac
    25681 agattctgat ggaatgataa tggaagaatt agaaaagata ctagaaacaa ctgatagaat
    25741 aaaaatgatt tacgtaatac ctgacttcca aaatccaaca ggaagaactt ggccacttga
    25801 aagacgtaaa aaattcatgg aaatagtaaa taaatttgaa ataccagtaa tagaagataa
    25861 tccatatgga gatttaagat ttgaaggaga gactttacca tctttaaaat caatggatac
    25921 aaaaggatta gtaatattct taggaacttt ctctaaaata ttctgcccag gatacagatt
    25981 aggatggact tgtgcttctc cagaaatttt atctaaattt aactttgcaa aacaaggtgc
    26041 agacttacaa gcatcaacta tatctcaaat ggaagtaagc aaatttatgg atatgtatga
    26101 cctagatgca catgttgaca aaataaaagc agtttatgtt aaacgtagag atgtaatgct
    26161 taagactatg gaagaagagt tcccagaagg attagtattt actcatccag aaggtggatt
    26221 atttacatgg gtagagcttc caagtaattt aaatgctaaa gaattaatgc caaaatgttt
    26281 agagaagaat gttgcttatg ttgctggagg aggattcttc cctaatggtg gtagagaaaa
    26341 tactttcaga cttaactatt caaatatgcc agaagaaaaa ataatagaag gtataaaaaa
    26401 tatagcagct gtattaaaag aagctatggg tgtagaagct taattaaaaa taataactta
    26461 tatataatac aagggatggt tgttgccatc ccttgtttgt ttgaaaagta tataattcac
    26521 cttatactaa actttgtcca aaatttaaat tgacagaaat aagaataaat gataaaatgt
    26581 gtaggtatga gattttagtc tcatttctct gctctaagag tgtagagccg ttaagtccaa
    26641 agggaggtga attatagtga gaaattatga attagtttat gtggtaaagc caaattctga
    26701 tgaagaagta agagaggcta tactaaacaa agttaaggaa gttgttgcaa ctgacggaga
    26761 aatagttaaa gttgatactt ggggaactaa aaaattagct tatccaatag ctaagtttac
    26821 agaaggtttc tatgtattag ttaacttcaa atcagcagta gacgttccta aagagataga
    26881 cagaaactta aagataaatg aaaacgtaat aagacacatg atagttgttg cttaataact
    26941 ggttttatta taaaaaatag gtggtgtttt tatgaatcaa gttgtattag tgggaaggtt
    27001 gactagagac cctgaattaa gatacatccc aggtacagga acaccagtgg catcctttac
    27061 aatagccatt gatagagact atgtaaaaaa agatggatct aaagaaactg attttatacc
    27121 tgttgaagta atggggaaat ctgctgaatt ttgtgctaac tacataacta aaggtagatt
    27181 agtagcactt caaggatcta taagagtaga caattatcag acacaatcag gtgaaaagag
    27241 aacttttaca aaagtaagta ctagatcagt acaagctttg gatagtaaaa ataaatcgga
    27301 aaattcatat agagagagtg ctccagcttt tgagccaagc tttgaacctc aaggtttaga
    27361 cccacaaggt tttcaagcta tagatgatga cgatatacca ttttaaaaag gagggaaaaa
    27421 agtcatgatg aataagaaaa gacgtaagaa aaaaagagtt tgtcaatttt gtgctgacaa
    27481 aaacgctaaa atagattaca aaagtactca aagattacaa aagtatataa ctgaaagagg
    27541 aaagatatta ccaagaagaa tatctggaac ttgtgctaag catcaaagag aattaacagt
    27601 tgctataaaa agagcgagaa atatagcact tttaccatat acattagact aaaaaaatga
    27661 gccttgtgct cattttttat tatatgtatt tatatatcta aatgaggcga gctataaagc
    27721 tcgttttttt gtttatatac ttaaatatat tattatttat gatataatat tatatattaa
    27781 ggagggttgt aaacgtgaaa atagataaag gttctgagat aacgagaaac ataaagataa
    27841 tagaatggat gaaaacagaa atattaatga gtgtaagtga tttgtttaat ttattgttta
    27901 aaggggtaaa accattagat gaagcaattc aagatacttt agcaaatata ataatgataa
    27961 catatctctt agcaaagaga ttaggaatta gttttagaga tgtggattat aaagtcaagg
    28021 aaaaaataaa aattggtata gatgaggacc atagcgttga aagatggtat ggagatttat
    28081 ctaatttaaa aaaacatatg gataataggg agtgatatta aattgaataa taaaattaga
    28141 ctatctaagg caatgtctat aatagtttta gctataataa tcgctttagt aatagcctat
    28201 attccaatgc taggaatatt tagtatgtta gcagctgttc catatgtaat tataggtgct
    28261 attactgata gaaagtattc tcttatatct attttggtta cattttgtgt actgattcta
    28321 tttgcagaca tttcttatgc attaaatata tgtataatgt attctttacc aggtatagta
    28381 ataggaaaga tgctaaagag aagctttgaa caagaggaca gtaataaatt cgaaccgata
    28441 tatggtggaa ccataatatt tgtactttca atgcttgtat atttttttgt attaaagaca
    28501 tttttaaatg tgaatctatt agatgaagtt tctaagatga tatcagaaat tgtaaatatt
    28561 caaaaaaata gtttttctgc aactgaactt aaagtgtttg ataaaatgaa gccagatgag
    28621 attgtaagtt attttacaaa tatgctacca atgatgttat ttttacaagg gttgttatca
    28681 gcgtttgtca catattattt gagtgtattt tttataaaaa ggattacaaa gatgaatata
    28741 agatttccta aatttgctga tttttattta ccaggaaatg ctatactcac aactttctta
    28801 ctttatttat tagtcttatt tattcaaatt attggttcaa aattatatac agaattaata
    28861 atgactaatt tacagctagt ttttaattta atgtttgtaa ttcaaggtat agcagtttgc
    28921 atttatttct taaagaaatg gataagacag ggaccaaata aaattatgtt tttttcaggg
    28981 ataattttat gtttatttgg atttatggga atatcttttg ttggtatggt agatagtata
    29041 attgacttta gaaaggtaag aagctataaa tccacttagg aggatagaaa tgagtaacaa
    29101 acaaaccttt aaattaaata tgccagaaat aaatttatat ataattgtta ttggtatttc
    29161 tagtataatt ttgctttatt acaacttgta tgtagggtgt ttatttttct gtatttttgt
    29221 atatatggtt tttcacaatt ggagaactac taatattaga cgtcatgaat ggacagagta
    29281 tattcaaaat ttatctttag atatagatga aacaactaaa aaagccataa taaatcttcc
    29341 aataccatta tgtattttag aatttgatgg aaatatatcc tggtataatg gaaagtttta
    29401 tgatatgatt ggtcaaaaag atttacttga taaaaatata gaggatatag ttaaaaatct
    29461 taatttaaga aaagtattaa atgaaaataa agagatgtac acagaaataa actataaaga
    29521 aaaagaatat acaataattt ataatgttat aaaaaatgat caagaaaaaa atcctaaata
    29581 tttgatgata ttatactgga tagacaagac tgaatattta aaagtaaaac aaaattatga
    29641 tgatgaaaaa aatgcaatga tgctaataca agtagatgga tatgatgaag tattaaaaag
    29701 tgcagcagaa gataagagag ctttaattaa tgttgaagta gagaaaatac tttctgcttt
    29761 agaattaaat tcaaatggtg cattgagaag aacttcaaaa gacaaattct tcttggtaat
    29821 gcataaaaaa gagctaaaaa aattggaagc tgagaagttt tctattttag atacaataag
    29881 acatattgat tatggaaata atcttcctgt tactataagt ataggaattg gtatagatgg
    29941 agatacatta aatgagaacc taaaacttgc tacgggtgct ctcgatttag cacttggtag
    30001 aggtggtgat caggctgttg taaaaacaaa agataaattt gtgttttatg gaggaaaatc
    30061 taaagctgta gaaaagaaaa ccaaagtaaa atccagatta ataggtcatg ctttaagaga
    30121 ggttatacaa caaagtgacc aagtttatat aatggggcat aaatatcctg atatggatgc
    30181 tatgggagca gcagttggtg tttatgatat atgtaaatcc tgtaacaaaa ctgcaaacat
    30241 agttttacaa tctgtaaatg aatctataga aatatttata aataaaataa atgaaaataa
    30301 ttactataaa aagctgttta ttggaaaaga ggaagctatt gacaactgta ctaaaaatac
    30361 attagttgta gtagttgata ctcatagacc aaattatact gaatgtgaag aactgttaaa
    30421 gctatcagag aaggtagtag ttatagacca tcatagaaga ggtgtggagt ttataaatga
    30481 tgcagtgctt ttattccatg agatatatgt atcctcaacg tgtgaaatgg ttacagagtt
    30541 agttcagtat atggatgaag atgtaacaat aaataaactt acagctgaag ggcttttagc
    30601 aggtataagt ctagatacta aaaactttgc attcaaaaca ggtgttagga cttttgaagc
    30661 tgcttcttac cttagaaaag ttggagcaga tactatagag gtaaagaaat tcttcaattc
    30721 tgatgtaaaa gatttcataa taaaagctga aataattcaa agtactaaaa taattaataa
    30781 tagaatatgt ttagcatatt caagtactga gatagatagt ataaatgtta taatagctca
    30841 aactgctgat gagttattaa atatcaaaga agtagaagct tcctttgtat taggtgaaaa
    30901 agatgatact atatttataa gtgcgaggtc tttagggcaa ataaatgtac atgtacttat
    30961 ggaaaaatta ggaggcgggg gacacataga tatagctgga gcacagttaa aaaatgtttc
    31021 tctaaaagaa gcttataaga tggtaaataa aataatagag gagtatttgg aggaggaaga
    31081 atagatgaaa gttatattat taaaagacgt aaaaggaact ggtaaaaagg gagaaatgaa
    31141 agaagtaagt gatggatatg caagaaattt cttatttcct aaaaagatgg ctgtgcaagc
    31201 agatagtgta gcaataaaag aattaaatga aaagaataaa tctaaagaaa taaaagcaca
    31261 aaaagagtat gaagaagcag ttttattagg taagcaaatg gaagaaatta atatagaaat
    31321 atattcaaaa gcaggagaag gtggaagatt atttggttca ataacttcta aagaaattgc
    31381 tgagcaatta aagaaacaaa aagacataga cgtagataaa agaaaaatat tattagatga
    31441 accaataaga tctttaggtt caactttcgt agaaataaaa atacatcaaa aagtaactac
    31501 taaaataaga gtggatgtaa aagaaaaaca gtaataacta gaggtgatta agatggaaga
    31561 tatgacgaga attcctcctc atagtgtaga atcagaacaa tcaatattgg gttctatact
    31621 tctagataaa gatgctataa taacggttac agaaactata aagcctgatg atttctataa
    31681 agaggctcac aaaataatat atgaatgtat gataacttta agtaataaag gtgagcctat
    31741 agacttgata acattaacag aagaactaag aaaacaaggg catcttaatg atattggagg
    31801 aatcagttat attacaagtc tttctactat agtaccgacg acttcaaatg taaaatacta
    31861 tgctgatata gtaaaagaaa agtctgtact tagaaagctt ataaaagctt ctaatgaaat
    31921 aataaatcta ggatatagtg gagcaactaa gattgaagat gttttagaac aagctgaaaa
    31981 gagtatattt gatatatcac aggagaaaac tagtgatgat tttaaatcta taaacttagt
    32041 tttgatggat gcctatgata tgatagaaaa attatatact aacaagagtg atgtcactgg
    32101 tataactact ggttttaaag atttaaacaa aaaaataaat ggtcttcaaa ggactgattt
    32161 aatattaatt gcagctagac cagcaatggg taaaactgcc ttttctttaa acttggttca
    32221 aaatgcagcc ttaaaaggtg atgcctctgt agctgtattt agcttagaga tgtctaagga
    32281 acaattagta caacgtatgt tatcttcaca atctagtgta gaattaaaaa aaataaagac
    32341 tggaacactt aatgataatg attggcctag aattatagat gctatggcag tattatcaga
    32401 tgccaaaata cacatagatg atactcctgg tataaaaata tcagaactaa ggtcaaagtg
    32461 tagaaagtta aaaatagaaa aaggactaga tttagtgctt attgattatc ttcaacttat
    32521 ggaaggtgaa ggtaataatg aaagcagaca acaagagata tctaagatat caaggtcact
    32581 aaaaatatta gcaaaagaat taaactgtcc tgtagttgca ctatctcagt tatcacgtgc
    32641 accagaacaa agagcagacc atagacctat gttatctgat ttaagagagt ctggagcaat
    32701 agaacaggat gcagatatag ttatgttctt atacagggac gaatattatc atgctgattc
    32761 tgagagtaaa aatataggag aagtaataat agcaaaaaat agacacggtg aaactggttc
    32821 tgtggaactt gtttggcttg gtgaagttca aagatttggg gataagttga gagacctata
    32881 atctaacgaa tatccacaga acgtcctttc tttctacagg aaaaatgtgg atacatgtca
    32941 ctaaatatgt ccaaacattg aaatttagct aaaaaactat ccacactatc cacagatagc
    33001 ctgttgataa gtgtgaataa gtagataaag caagcttttg gcttgcttta tctgtgtata
    33061 aatattaatt attaggagga ataatatgga aaaaataagc ttaaaatata tatatcccaa
    33121 tataattaaa gtacttgatg aaataaactt atttagagta attgacaata atttaagaga
    33181 gtctattgtt gtttatgcta ataatgtgga taatcagtat catataaaca tgactaatac
    33241 aaactttgga aacataatta acatatgtaa attagaaaaa cttttagatg tggataaatt
    33301 tatggaaaaa gttataaaat atgaaaaaga aataatagag aaagaagagt tttcaaaaat
    33361 agaagaatat atgttaaata ttggagaata ctaaaaaagt tgaaaaaaat acgaatgttt
    33421 gatagaataa gtaagtaaag tgttcgagag tatataaaat atagaggtga attacatgaa
    33481 aacagttgca attgttggtt ctcaatgggg agatgaaggt aaaggtaaag ttatagatta
    33541 tcttgctact caagcagatg tagtagtaag agggcaaggt ggaaataatg caggacatac
    33601 attagttgta gaaggtaaaa aatacgcatt acatctaatt ccatctggag ttttaaatcc
    33661 aaatacagtg aatataatcg gaaatggaat agtgtttgac cctaaaggat ttttagaaga
    33721 attggaaatg tttaaaacgg ataatataag tacagaaaat ataaaaataa gtgatagagc
    33781 acatgtcata ttcccatatc ataaagaact agatgcattg tcagaagaag ccagaggcga
    33841 tttaaagata ggtacaacaa aaaaaggtat tggaccttgt tatatggata aaacagagag
    33901 atctggtatt agaatatgtg acttaatgga taaagataaa tttgctataa aattaaaagc
    33961 tcaaatagat gctaaaaata aaatagttaa aaatatatat ggaaaagaag aattatttga
    34021 ttttgaaact atatataacg aatatctagg atatgcagaa caaataagaa aatatgtggc
    34081 agatacgtct gttattgtat atgatgcagt aagagcaggt aaaaaagtat tgtttgaagg
    34141 agcacaaggg actttattag atttagattt aggaacttat ccatttgtta catcttctca
    34201 tccaacatct ggtggatttg caattggtgc aggtatagga ccaaatatga taaaagacgt
    34261 tgttggtata gtaaaagcat atactacaag agttggagaa ggaccgtttg ttacagagca
    34321 aataaatgaa actggagata agataagaga acaaggacat gaatttgggg taactactgg
    34381 tagaccaaga agatgtgggt ggtttgatgc agttatagtt aagtatgcag ctagagttaa
    34441 tggattgact agtatttcat ttatgttact tgatgtatta actggatttg ataagataaa
    34501 agtttgtacg tcttataaaa tgggcgataa gataataacg gatttcccag cttcattaga
    34561 tgatttagca aaatgtgaac ctgtatatga agaattagat ggatggaatg aagatataac
    34621 tcaaatagat aactttgatg accttccaga aaatgcaaag aaatatgtag caaaaataga
    34681 agaactagtt ggagtaagtg tagatatggt ttctgttggt ccaaatagag ctcaaactat
    34741 aataagaaga aatatatttg cttaatattt tatcaattaa ataatttaaa atagttgaag
    34801 aagagtatct caagataatg tttatagtta ttagagatgc tcttttatat ttgacttaca
    34861 ttgatttttt attaagttaa agctacttat acatattcta gctgaaatta aagttgaatt
    34921 tatgtagata catataggtt ttttaaaaag aattttgttg aaaataaatg gctaaagatg
    34981 attttaatta tattctaaaa aaagtaaaaa aaactattga ctaaaaattg tatagacgct
    35041 ataatataac ttgtgagtga aagaaacgac gaaaaaagtc gaaaaaatgt ggtctattag
    35101 ctcagtcggt agagcacctg acttttaatc agggtgtccc gcgttcgagt cgcggataga
    35161 tcaccaaaat atggctcgtt ggtcaagagg ttaagacacc gccctttcac ggcggtaaca
    35221 ggggttcaat tcccctacga gtcaccagta tgggactata gctcagctgg gagagcacct
    35281 gccttacaag cagggggtca caggttcgag ccctgttagt cccaccaaat tttgaattag
    35341 agataaatac tagattatat gctggtgtgg ctcaacggta gagcagctga cttgtaatca
    35401 gcaggttgta ggttcgattc ctatcaccag ctccacaaag tacataaatt caaattaata
    35461 taattttcta tgttacagga tacttttaag tatctttttt taatttcatt tttattgtat
    35521 taaaaatgaa attaaacata caacaaaatt ttgaattact tgcaatgtga tagttttaaa
    35581 tataaaattt agaatcattc tagcatagta ttatgtaata tagcaaattt aatttatagt
    35641 atcaataaat atagactact aagaatgtaa atgatttgtt agattaagat acattagata
    35701 tgtaaataga aaaataaaaa atatgtttta atttatgtgg cataagtttt aaacagagaa
    35761 gcaagtttgc tcttacctct ctatttaaaa ataaaaaaat gttttttaag ggctatttta
    35821 tttaaatctt tcccatctca aatcatctcc agtaaattct aaaatcctaa acttatctaa
    35881 gattctagag agagttcttt gagtgtaagt atttcctatt tgagatggtg ttaagttggt
    35941 tgaaataatg atttttttac cagcaacaag tcttgtattt acaatattga agatttcccc
    36001 actagtaaaa gaattattaa gttcagttcc aaggtcatct attattaata aatcacaatc
    36061 aaacaagttt ttataattat cttcacttat ttggttattt gtgtctcttc taaatttata
    36121 atcttcaaga atatctaaaa ttctaaaaga agtttgatag attacaacat ttcctttatc
    36181 taacaattcc tttgcaatac aattgcacat ataggtcttt cctagtccag tagaaccata
    36241 aaacagtaga ttttcactat tatccttctt aaaatcatga acaaaatttt cgcagatact
    36301 taaattattt agcatatttt ctctaggaga tatttcccca tcagagcctt tcttaggaga
    36361 aaatatattc aagttaaaat tcgaaaaatt ttcctgactt aaaatcctat ctaaattaga
    36421 cattttataa gcttcattta caatttcttg tttgaggcaa gaacattttt ctccattagg
    36481 taaaaatcct ttatctttac aagaaataca ttgaaacttt atttccaagt aatcaagagg
    36541 tatgttactt tcagataaaa gtttttcttt ctttactttt aaagactcta tattctcttt
    36601 agtcttcttt acaatttcat ctttagattt gggatttaaa agtactatct tagctaaact
    36661 gagacctatt ttagaaattt catcatctat agattttatc tcagggattc tattatatac
    36721 ctcatttttt ctatgttcta ataagagttc attatcatct cttctcttag catatttagc
    36781 aagtattttt cttattttat cctcattcat ttaatcacct acttaaactt ttctttttga
    36841 ctttttttaa taatctcatc gagctcatca gaggtatact gagtgaatgt ttcgttgaaa
    36901 ttatgaaact tagttttttt gtaagtattg ttgttttgag tagtattaat ctgcttctta
    36961 ttaatattct ccttaactat acgttcttcc tctttttgct ttaaatcatt taaatttttt
    37021 atattttttt cattccaatt tttaattata ccatttatat aagatatact tgggtttgaa
    37081 atattcttag acttagaaca tgcatttatt atcaaatcta tatccatatt aaatttatca
    37141 aaccaggtgt ccataagttc tttttctgat tttgatggtt gtcttgaaaa tcccaattca
    37201 ttgaaaatcg ttttatataa aatatatcgt tcacttctta ccaaaaagct ttcttcaaca
    37261 tcttttggcg tatataaatt tgaatcatac caatttctaa taatgccttc aatgtattta
    37321 actggctttg atgtaccagt tttatctttg acgtattcat atgcgcaaag tatcatatcg
    37381 gggctcatat tgtatttatt cattatatcc ataatactca tcttttcact tgggtcgaga
    37441 tatcgcccaa ctattttatt tatagaatta aacatttttc taatacttgg attttcagaa
    37501 gtagatacaa ccttatctgt attagatttt atagatgaat tattgcttaa tatattctca
    37561 atatagaaat tttttaaatc taaaaattct attgaataat taaaattatc atattctcca
    37621 ttgtcatgta tttttataat cttcttttct tcccagaatt tccaagcaga taacacatca
    37681 gacaatggta tatttaggtt tttggctatt gaattattgt cgaacttagg attagaagta
    37741 atatcacatg cttgtctata tcctaaaaga taaactttta cgtataaccc gtccgccatt
    37801 ggcataaata tatctataaa gatattggga atagtgattt cacctaaatc tatttcattg
    37861 ctttctttaa aaaacatttt atcacctcag attaaattat atcatttttt atattatatt
    37921 tgtctgtaaa caaataaaag gtcacaagat tagcatgtaa atattttcta aaagtattca
    37981 ttttgaaaag gaggagatat tttggttata aaattcaacc aaaaagtaag aagaacatta
    38041 tcagtagcga ttctatttat ggtattaata ataggttgct ttatagaaaa ggatgacagt
    38101 ttagaaacat acaaagaaaa aactaattca aaaataaatc agtataatat ggatgttata
    38161 tttgatgatg agactaagag gttaatgtgc aatcagaatg ttgactacat aaataataca
    38221 aaatcaaaca tagataaaat atatttccat atatatccga atgctttttc caagaaagat
    38281 tttgctcctt ttgaaaaaag tgaaatgaca agggcttatc ctaatggatt taacgaagga
    38341 tatatagata taaagaatat attaaataat aatagtaaaa tggagtataa aattaaagga
    38401 gacaaaaatg acatattaga aatagaatta gggaaagagt taaaaccaaa tgaaagaatg
    38461 tcattagata taaaatataa cgtaaaaata ccaaattcaa ttggaagatt tggatatgga
    38521 gaaaatacta taaatgttac taattggttt cctatagctt gtgtaaatga tgaaagggga
    38581 tggaatttaa agagttatga aactattggc gacccatttt atagtgagac tagcgatttt
    38641 catgtgaaac tattgattcc aaataaatat aaaattgctc atacaggaaa acttattgat
    38701 gataaacatg ataataagaa aatgctatat gaaatagaag ctgaaaacgt tagagatttt
    38761 gcatttatac taagtagtaa atttgatgta aacaaagtag attcaaaggg agtagctatc
    38821 aatacatata atttaaataa aaagctttct aaaaaagcaa ctgaagtggc aaaagattca
    38881 ataaatatat ttagtgaatt atttggaaag tatccttaca aagaattttc tgttgtagca
    38941 agtgactttt ttataggggg gatggaatac cctatgcttg ttatgataga ccaaagttta
    39001 tataatgaaa aaaatgaatt tttactagaa tatgttattg cccatgagac agcacatcaa
    39061 tggtggtatt ctgctgttgg aaatgatgaa ataagtgaac cctggttaga tgaagcactt
    39121 actgaatatt ctacaatagt ttattttgaa caaaagtatg gtaaagatat ggcaaacaag
    39181 cttataaaaa ctatggagat acagacaaaa agttatttga gtgaaaatat atttaagcca
    39241 gcaaatgagt ataaaaattc tactgaatac agcttgaatg tttatacaaa aggcgcaata
    39301 gcatttaatg aaataagaaa agaagttgga gataaagtat tctttgagac tttaaatgaa
    39361 tactataata aatataagca taaaaatgct aatggaagag cttttgtgga actatggaat
    39421 agtaaaggtg tagatataaa taagattata agtgaatgta agtaaatagt gaatgtaagt
    39481 aaattttgtg cagagtatta aattttattt aatactctgt ttttttggta taatataatt
    39541 tataaggcag aaaaagaggt gaaggaatgc taaagtattg ttcagtaggg agtggtagta
    39601 gtgggaattg ccactatata gggtataaaa atactaatat actagtagat gcaggattaa
    39661 gtggcaagag aataactaca ggtcttaaag acattggtgt agatgcagat aaattaaaag
    39721 gaatatttat aacacatgag catgttgacc atataaaagg agcaggtata ttatccagaa
    39781 agttcgatat accagtattt gctaatataa aaacatggtg ttctatgaag gacaaattag
    39841 gtgacatcaa agataaaaat atgaaagtat ttgaaaatga taaaacctac tcattaggtg
    39901 atatcatagt gagacctttt tcaatagaac atgatgcgtc tgatccagtt ggatataatt
    39961 tttatacaga gagtgatgaa aaaatgtcta tagctactga tataggatgt ataactcaaa
    40021 atataaaaaa acatctatat aaatctaaat tagtagtatt ggaatcaaat tatgacccta
    40081 atatgcttat gatgggttca tatacatatg ctttaaagaa aagagtaatg tcaagtactg
    40141 gtcatttatc caatgaagat gcagctaatt tctgcgttga acttattaat gaaggtacag
    40201 aatctatatt attagcacat ttaagtaaag aaaataattt tcctgagtta gcatatgaga
    40261 cttcaaaagg agtattagca tcaaatgata tagtagttgg acaagatgta aaactagatg
    40321 tactatcacg aaatgatgtt tctaatgttt atgagatgaa gtagaggtga ataactttgc
    40381 taaagagaaa ttttgaaaat gttgaggata ataaaatttt atactctata aagcagggta
    40441 tgattttagc aatacctgct attatgactg gttcaacggc tcttgtaatt ttgaatcttc
    40501 caataaaggc ttatcaagaa tatttatcag gcttgtttaa tggagaggtt acaaatattc
    40561 ttaactttat aaatgattct actttaggaa ttatttcatt aataatatta ttaacaataa
    40621 gctatagtta tggaaaaata tatgggagca agtatacagt acttgttcct atagttgcaa
    40681 tgtgctcttt tttagtattt tcacatggta atgagcttaa ttcttatata gaaatattta
    40741 agacaaagtg gttatttaca tctataattg tttctatgac atcttctgta ttgtttgtaa
    40801 aacttacaga atcttttgtt acaagtgtaa agtttcatac agaaggggct gatgcagact
    40861 ttaatatggt agtttcggct atagttcctt tcataatagt agtatttttg ttttctattt
    40921 ttagagtgat aatgataagt ctaattggca gttcaaactt tcaagatata ttttataatt
    40981 tattttcaaa tgtttttaat aaaatgggaa caaaccttat gagtgctctg ttgtttattt
    41041 ttttaatgca ctttatgtgg ttttttggga ttcatggtag taatgtatta gataccgtcg
    41101 caaaaaattt atttgaaaat agtatggcaa ttaacataaa tttagttaat aataatcaat
    41161 taccaacaga aatttttact aagacctttt ttgatacaat ggttttattg ggaggatgtg
    41221 gctctttact atgtttagta attgcaattt ttcttagtga aaaacgtata aatgtaagaa
    41281 agctagctaa aattgcttca ataccagctc tttttaatat caatgagatg ctagttttcg
    41341 ggataccaat tgtttttaac tatattatgt ttgtaccttt tgtaattact cctataatct
    41401 taaccattac aagttatgtg gctatgtcta caggtattgt accatgtaca gtttcagctg
    41461 tagaatggac ctcacctata tttttaagtg gatatatgtc tacaggttca ataagaggta
    41521 gtttgttgca agtatttaat ttatgtgttg gtgttatgat ttatattccg tttataaaaa
    41581 tgtcgcaaaa tagatataca tatatgttta agaataatat agaaaattta acaaacttat
    41641 taaaaaggta tgaattaaca ggtgaacagc ctaatcttct aaaccataat ggtaaaatag
    41701 gttcaatttc aaaaatgttg gcatcagact tgaagtttgc tatgaaaaga ggagaaatag
    41761 aactttttta tcaaccacaa gtcaattata atggaaatgt tgtgggagca gagggattac
    41821 ttagatggaa acattcaata ggagggttca tttatccacc actagttata attttggcta
    41881 aagaagaagg gttcttagat gagcttggag agtatattat taataaagca tgtattgatt
    41941 taaagaagag tgaaaagcta ttgagaaatc ctgttaagtt ctctgtaaat atatctccag
    42001 accaacttga tgaccctaag ttaccggagc aaataaaaaa tatcattagt agtaatgata
    42061 taaatcctaa tatgttaggt atagaaataa cagagcaaat tgcattatca ggttcacaaa
    42121 taattattga acgtatcaat gcgatacata acttgggaat aaagttaatt atggatgatt
    42181 ttgggatggg gcatagctct cttatatatc ttcaaaataa taactttgat atagttaaat
    42241 tggatggttc attagttaaa gagatactta caaataaaag aagtagtgaa attataatga
    42301 ctattgtaaa tctttcaaaa aatttagatt ttgatatagt tgttgagtat gttgaaaata
    42361 tagaccaaag ggataagtta tatggtttag gatgtgaaat atatcaagga tattattata
    42421 gcaaagcaat accttttgaa gagtttgtag aatatgcaaa tcaagaaaaa gagatataat
    42481 tttataagag cttgtaaatt ctagtaatgc atagatttgt aataaaatta agtttaggag
    42541 aaatatatga atataagtat agtagtagta ggtaaaatta aagaaaaata tttaaaatta
    42601 ggtattgatg agttcaaaaa aaggctttct aagtattgta agttagaaat tatagagttg
    42661 gatgatgaaa aagctccaga gaatttgagt gttaaggaaa tggaaattat aaaagacaaa
    42721 gaaggaaaga aaattttagg gaagataaag cataatagtt atgttatagt cttggctatt
    42781 gatggtaagg gtttatcctc tgaagaactt gctaagacta tgagtgattt agcagttaga
    42841 ggtaatagta gtctctgttt tataatagga ggttctttag ggctttctga tgaggtttta
    42901 ggaagggctg attataggtt atccttttct aagatgactt ttcctcatca gatgatgaga
    42961 ttgatattgt tagaacagat ttatagggct tataggataa ataatggaga accgtatcat
    43021 aaataaatga tacggttttt gctaatttga cacttaataa tattaaaata acaaaaaagt
    43081 attgaaagta tattgcattt aaggtagaat taaataaaat attataacat tataatgttt
    43141 atatagttgt taataaaaat ttggaggaaa atatatgata aaaataatgt tggcctgttc
    43201 agcaggaatg tctacaagtt tattagttac taaaatggaa agtgcagcta aagaaaatgg
    43261 tatagagtcg caaatttggg caataccaga atcaactata caaaatgaaa tagaaaaatg
    43321 tgatgtatta ttactaggac cacaagttag atatgtatta cctaaggctc aagaaattgc
    43381 taagccatat aatataccag tagaggttat aaatatgatg cactatggaa cagttaatgg
    43441 agaagctgtt ttaaatagag cgatagaact taacaacact aagtaatata aattataatg
    43501 tatttttaat atataaattg ggagaatcaa aggatggatg aaaagattat agaaatatct
    43561 tttaatataa taggttatgc aggtgaaggt aaagggttag cttttgaagc aataaaagag
    43621 gctaagaatg gaaatataga aaaagcaaaa gaattattaa aagagtctaa ggaagttata
    43681 aataaagcac atagatatca aacagaatta atacaaaacg aagctagtgg aaacaggact
    43741 gaaataagtg tcatacttat acatgcacag gaccacctaa tgaatggtat gaattttcaa
    43801 cagttagctg aagaaatcgt tgatttacat ttgaaattac aagataaata aaaattaaag
    43861 agaataaatt aagagagttc ttaaaagata cattatgtgt agcacactaa ttgttagatt
    43921 attgattcaa tatttagtga gtaatacatt aggattattt tgaggactct ttttatattt
    43981 agtttaaagt taataaatat ttattattaa ta
//
LOCUS       DAGQWR010000013        41262 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.013, whole genome shotgun sequence.
ACCESSION   DAGQWR010000013 DAGQWR010000000
VERSION     DAGQWR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.013"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(191..1546)
                     /locus_tag="MIW95_000631"
     CDS             complement(191..1546)
                     /locus_tag="MIW95_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBY3149980.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPISTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(1572..1841)
                     /locus_tag="MIW95_000632"
     CDS             complement(1572..1841)
                     /locus_tag="MIW95_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBY3149981.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(2131..4071)
                     /locus_tag="MIW95_000633"
     CDS             complement(2131..4071)
                     /locus_tag="MIW95_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149982.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCNMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAKDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNQLDIDVEWDFTDYYEKLEKGENFDITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKTIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNSDEKQPNTPKDD
                     VESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNPITENDDNYDLSSKSVFGTNEH
                     SFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCILTPIMI
                     GSILIVNPDTRKSIIKFIKLKK"
     gene            complement(4255..5730)
                     /gene="spoIVA"
                     /locus_tag="MIW95_000634"
     CDS             complement(4255..5730)
                     /gene="spoIVA"
                     /locus_tag="MIW95_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBY3149983.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(5902..7197)
                     /locus_tag="MIW95_000635"
     CDS             complement(5902..7197)
                     /locus_tag="MIW95_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3149984.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNKSNENTTKVS"
     gene            complement(7381..8790)
                     /locus_tag="MIW95_000636"
     CDS             complement(7381..8790)
                     /locus_tag="MIW95_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY3149985.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(8842..9981)
                     /locus_tag="MIW95_000637"
     CDS             complement(8842..9981)
                     /locus_tag="MIW95_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY3149986.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            10300..10950
                     /locus_tag="MIW95_000638"
     CDS             10300..10950
                     /locus_tag="MIW95_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY3149987.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSGDDELTMKMISSIEYIIKHAK"
     gene            complement(11107..12126)
                     /locus_tag="MIW95_000639"
     CDS             complement(11107..12126)
                     /locus_tag="MIW95_000639"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBY3149988.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVINKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(12157..12771)
                     /gene="plsY"
                     /locus_tag="MIW95_000640"
     CDS             complement(12157..12771)
                     /gene="plsY"
                     /locus_tag="MIW95_000640"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBY3149989.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(12806..14125)
                     /gene="der"
                     /locus_tag="MIW95_000641"
     CDS             complement(12806..14125)
                     /gene="der"
                     /locus_tag="MIW95_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBY3149990.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(14159..15487)
                     /locus_tag="MIW95_000642"
     CDS             complement(14159..15487)
                     /locus_tag="MIW95_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBY3149991.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(15725..15925)
                     /locus_tag="MIW95_000643"
     CDS             complement(15725..15925)
                     /locus_tag="MIW95_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3149992.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(15927..16859)
                     /locus_tag="MIW95_000644"
     CDS             complement(15927..16859)
                     /locus_tag="MIW95_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBY3149993.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNM
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(16904..17836)
                     /locus_tag="MIW95_000645"
     CDS             complement(16904..17836)
                     /locus_tag="MIW95_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBY3149994.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYDLSFFHCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNDVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(17891..19180)
                     /locus_tag="MIW95_000646"
     CDS             complement(17891..19180)
                     /locus_tag="MIW95_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY3149995.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(19209..19901)
                     /locus_tag="MIW95_000647"
     CDS             complement(19209..19901)
                     /locus_tag="MIW95_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3149996.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(19916..20677)
                     /gene="pgeF"
                     /locus_tag="MIW95_000648"
     CDS             complement(19916..20677)
                     /gene="pgeF"
                     /locus_tag="MIW95_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBY3149997.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENITSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(20674..21135)
                     /gene="nrdR"
                     /locus_tag="MIW95_000649"
     CDS             complement(20674..21135)
                     /gene="nrdR"
                     /locus_tag="MIW95_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBY3149998.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIEEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(21157..21420)
                     /locus_tag="MIW95_000650"
     CDS             complement(21157..21420)
                     /locus_tag="MIW95_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBY3149999.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(21524..22297)
                     /gene="sigG"
                     /locus_tag="MIW95_000651"
     CDS             complement(21524..22297)
                     /gene="sigG"
                     /locus_tag="MIW95_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBY3150000.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(22389..23111)
                     /gene="sigE"
                     /locus_tag="MIW95_000652"
     CDS             complement(22389..23111)
                     /gene="sigE"
                     /locus_tag="MIW95_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBY3150001.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(23136..24008)
                     /locus_tag="MIW95_000653"
     CDS             complement(23136..24008)
                     /locus_tag="MIW95_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBY3150002.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGSAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(24299..25573)
                     /locus_tag="MIW95_000654"
     CDS             complement(24299..25573)
                     /locus_tag="MIW95_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBY3150003.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(25857..27014)
                     /gene="ftsZ"
                     /locus_tag="MIW95_000655"
     CDS             complement(25857..27014)
                     /gene="ftsZ"
                     /locus_tag="MIW95_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBY3150004.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(27204..27557)
                     /locus_tag="MIW95_000656"
     CDS             complement(27204..27557)
                     /locus_tag="MIW95_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBY3150005.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(27656..28330)
                     /locus_tag="MIW95_000657"
     CDS             complement(27656..28330)
                     /locus_tag="MIW95_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBY3150006.1"
                     /translation="MKKKIVILGAFILGIFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(28335..29033)
                     /locus_tag="MIW95_000658"
     CDS             complement(28335..29033)
                     /locus_tag="MIW95_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBY3150007.1"
                     /translation="MKKRFINKSIIIGSVVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKTEKENLQSEVNSVKKDIETYRGKNNKGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVFNTYMY
                     LKEDKLCMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(29056..29796)
                     /locus_tag="MIW95_000659"
     CDS             complement(29056..29796)
                     /locus_tag="MIW95_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBY3150008.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNINLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSVDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(30066..31292)
                     /gene="murG"
                     /locus_tag="MIW95_000660"
     CDS             complement(30066..31292)
                     /gene="murG"
                     /locus_tag="MIW95_000660"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBY3150009.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(31310..32440)
                     /gene="spoVE"
                     /locus_tag="MIW95_000661"
     CDS             complement(31310..32440)
                     /gene="spoVE"
                     /locus_tag="MIW95_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBY3150010.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(32492..33847)
                     /locus_tag="MIW95_000662"
     CDS             complement(32492..33847)
                     /locus_tag="MIW95_000662"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBY3150011.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEVVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(33866..34834)
                     /locus_tag="MIW95_000663"
     CDS             complement(33866..34834)
                     /locus_tag="MIW95_000663"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBY3150012.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(34875..36248)
                     /locus_tag="MIW95_000664"
     CDS             complement(34875..36248)
                     /locus_tag="MIW95_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBY3150013.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREASAQNAFVA
                     IVGENLDGHTFMKPAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDIYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(36415..38394)
                     /locus_tag="MIW95_000665"
     CDS             complement(36415..38394)
                     /locus_tag="MIW95_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBY3150014.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(38406..38753)
                     /locus_tag="MIW95_000666"
     CDS             complement(38406..38753)
                     /locus_tag="MIW95_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150015.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIAIFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(39239..40174)
                     /gene="rsmH"
                     /locus_tag="MIW95_000667"
     CDS             complement(39239..40174)
                     /gene="rsmH"
                     /locus_tag="MIW95_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBY3150016.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGKLVDIIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNKGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(40204..40950)
                     /locus_tag="MIW95_000668"
     CDS             complement(40204..40950)
                     /locus_tag="MIW95_000668"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBY3150017.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKDDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKHNISEE"
BASE COUNT        12724 a         7728 c         4290 g        16520 t
ORIGIN      
        1 attaaaataa aagcgataca tgaattttta aatgtccatg tatcgcttta tttttgccat
       61 aatcttaaca actgattttt aaagtttatt atcttaacga agttcattat cttatataat
      121 atttaaatgc atagacttct ttaatttaac catctaaata tttaattaaa tattattata
      181 tgtaatataa ctaattttta aatgaatgta taggagctgg aatccttcct cctctttgag
      241 taaataattc agatgaattt gtacttatag gcattactgg tgctcgtcct aaaagtcctc
      301 caaattctac catatcacct acatcacatc ctggggctgg tataattcta acggctgtag
      361 tcttattatt aatcatacct attgctgcct catcagctat catagctcca agcgtacctg
      421 ctgtagtatt tcctggcaca gctatcatat caagacctac agaacaaata gcagtcattg
      481 cttcaagctt atctatactt aaagcacctt taataacagc atcaatcata cctgcatctt
      541 cagatactgg tataaacgct ccactaagtc ctccaacatg tgaacatgcc attactccac
      601 cttttttaac agcatcattt aaaagtgcta atgctgctgt agtcccatga gtaccaacaa
      661 cttcaagacc catttcctct aatatatttg ctactgaatc accaatagca ggtgtaggag
      721 ctagtgataa gtctattatc ccaaatggaa catccaatct actagaagct tcctttgcca
      781 caagttgtcc aactcttgta attttaaatg cagtcttttt aactgtttct gaaacaacat
      841 caaaaggctc tccctttact ttctctaaag ctctcttaac aacaccaggg ccacttacac
      901 caacatttat aattctatca gcctcaccaa caccatggaa agctcctgcc ataaatggat
      961 tatcttctac tgcattacaa aatactacta attttgcaca accaaaacct tttgcatctt
     1021 ttgttaattc tgcagtttct tttatgattt ctcccatttc tttcactgca tccatattta
     1081 taccacatct tgtagaacct acatttactg aagcacatac tttattagta cttgctagag
     1141 cttttggaat tgacttaatt agtatcttat caccttttgt atatcccttt tgaactaatg
     1201 ctgagaatcc accaataaaa tctataccta aatcctctgc agccttatcc aatgtctgtg
     1261 caaactttac atagtcattt tcatctgttg cacctgcaat aatggaaatt ggagttactg
     1321 ataccctttt atttacaatt gggataccaa attcacgctc tatatctttt ccaaccttca
     1381 ccaaatcttt agcagaactc ataatcttat tgtatatttt ttctctagct ttatctccat
     1441 cagggtctat acaatctaat aaagaaatcc ccatagttat agttcttata tccaactgtt
     1501 cttcatctat catctttatt gtttcaagta tatttcctac attcactatt atcacctccc
     1561 cttactgtct actacagatt atgcattgaa ttaaatattt cttctcgctg aatttttaca
     1621 tccactgata atttcttacc tctttcaacc aagtcctcaa ccgttttctc aaaagaattt
     1681 aatgatttct ctaaatcaac tatcataatc atagtaaaat aatcctgtaa tattttttgt
     1741 gatatatcta ctatgttgat attttgcttg tataattcat cactaattcc tgccactata
     1801 cctggtttat cctttccaat tactgttaaa attgctttca taataatatc ctcccttatt
     1861 tttttataaa tcttatattt ttacaaattg aactatgtat taacatatat ttcatttatt
     1921 tgttagtaaa ccacttttta tgtatatatt taatatattt taatacctat acattcattt
     1981 gtcaattttt tttcaatatt ccattatttt tatactattt agaaaattca tattttatat
     2041 atgttttctc aacaataaat acagttttca taataatgaa aggtattact tagcaatctc
     2101 gtgattttct aaataatacc tctctaaaaa ttattttttt aattttatga atttaattat
     2161 actttttcta gtatcaggat ttacaattaa tatagaacca atcataattg gtgttagtat
     2221 acaaaataaa aaaataatat ctttttttag attatattct ttagattcta atttataatt
     2281 attatttatt attccatcat aatcacttat atttgactta tttttatttg tatcttgatt
     2341 tttgttagaa atactttccc tataaggaaa agaatgttca ttggtaccaa atactgattt
     2401 tgagcttaaa tcataattat catcattttc tgtaattgga tttttattgt aaaatccttt
     2461 ttctttatca gaattatttt ctttattacc gtctttaggt atatcatgag gatttttatt
     2521 ttcattgcta tcagtacttg attctacatc atctttagga gtatttggtt gtttctcatc
     2581 agaattatta ttttcattat tattgttttg attgttatta ctatcattat tttcatttcc
     2641 attattccca tcttgattgt tatcatcctc atctctatcc gggtcgtctc tacctcctcc
     2701 tccacggtca ccatcaatat catcattttc ccgttcatca tctggcgtct cttggttgtc
     2761 tccgcctccc attataaatc ctggtttaaa aactatattt gaataccctt cttttaatcc
     2821 tcctaccact tttactctaa aacaacgcaa cttaaaatct tcaaaaaaaa gtatagcttg
     2881 atctctaata tcctcctgtt cttcagatat ccaattgatt ccatcgcttg aatattcaac
     2941 aaaaacctta cttgcactat acggcatatc ataaaataaa acagctacat atccattttt
     3001 tgtattttga aattccagtt ccaaattgtc tattgttttt ttctctataa cagatacatt
     3061 caaaatagga actacatcat catttattat aattttttct ttatttaaca tttctgtttt
     3121 aaactttcct gttatatcaa aattttctcc cttttccaac ttttcataat aatcagtaaa
     3181 atcccattct acatctatat caagttgatt accactagat gtttctaata ccatatattt
     3241 gggaaatttt atattttcta ttttatcatc tttacttact tttatttcat cactaaatac
     3301 accaattccc ctatgacaac atttcacaat attatagcca gaatctttag ctatagaggg
     3361 tataagctca ttataatcat caattatata aactttctta ctttctgata taattgattg
     3421 tgccaactta ttattatctc ttagtaaccc actttcattg ttgctactat atgcttttat
     3481 tgaagtttct tctatctcaa catcaccttt agaataaaca cctatagcat gttctccatt
     3541 cacttcaata tccttattta gtattctaat atcattttca gtataaattc ctattgcatc
     3601 ttttccattt gcctttattt tttcaacaga ttcgtaggat gatttaatat gtaagtctcc
     3661 tccttcttca acgtataagg ctgtcccatc agtagcaatt atgttgttta taccaatact
     3721 tatacttcca ccattttcta tatgtattac tccttctttt cctccttcac ctattatatt
     3781 caattcatta tcatcaataa taaatctccc atgatttttt atcaatattt tatatttgtt
     3841 ggtatctatt gtaaaattac tgtcataagg aatataaaat ctaaactctt catctattac
     3901 catattacaa gaaagagtat aaactatttc ttctttatta tttttatttt cttgaaccca
     3961 cctttcaaaa tcttcaagac aattttcctc ttgtccaaaa actttatgtg gaaaacttat
     4021 aaaacacaaa actattatac tcataaaaat aaatatctca tactttttca tcttacactc
     4081 ctagaaattt tttagctttg aaacaatcct gtcgaaacat atacttcacc cactataatt
     4141 tttccacaat tatcaattat tgtcaattta tataagtatc tttattaatt gttataaata
     4201 aaaagctact tactaaagta aacagacgtt tcactttaat aagtagctat atttttataa
     4261 caaaatagtt ataatattag aacttccttc atttacaatt ttttgtaaag tcttttgcat
     4321 tttaactctt atctcttctg gcatagtata taatttactt tgtaattgct cttttactaa
     4381 gtcatgtagt gacttaccaa acatatttga ttcccataaa tctgctggtt gctcttcaaa
     4441 tacttccata agatatttta tcatctcttc accttgattt tgatttccaa ctattggaga
     4501 gacctctgta gatatatctg cttttataat atgaagtgat ggtgcattgg cttttaactt
     4561 gataccatat tgttttcctt gtttcattat ttctggttct tccagactaa gttcttctaa
     4621 agaaggagct actactccat atccttttat cttggcatct attaaagcac tttctatttt
     4681 atcatactca tttttaactt tagatagtct agttatcaga ctaagcagtt gataatctcc
     4741 ttcaatttta aatccacttt tttcttcaag gacattatag aataaatctt gctttgtctg
     4801 taaatcaata tttataacac cttctcctag ctctacatta tcaacacctg tatcttccaa
     4861 aaactctaac tcagaaaatc cttgtataat accttctata tctcttattt tacctatatc
     4921 tataatactt tgttttaatg ttgttattat gctacttttt atccaatgat ttctttcaag
     4981 accttcaacc cattttggta agttgattct tatttctgta agtggaaaat catataatac
     5041 agcttccata acctcctcta tatcttcttc ttccatttca accacattca ttggaagaac
     5101 tggaacttca tacttttctt ccaattcact tctaagcata ctcgtttcct cactttttgg
     5161 acttaacgta tttaaaacca ctgcaaatgg ttttttaaga tttttcaatt cttgtataac
     5221 tctctcttct ggttctacat agctttttct gtcaattcca gtaactgaac catctgtcaa
     5281 gacaactatc cctattgtag aatggtcttt tataactttt ttagttccta tctctgctgc
     5341 cttttcaaat gtcatagctt cttgtgacca aggagtggat actaatcttt gttttccacc
     5401 ttcctcatgc cctaaagctc cttcaactat gtatccaaca caatctacca ttcttacttt
     5461 taaagatact gtgtctttta tttttatttc tacaccatct gctggtacaa attttggttc
     5521 tactgtcatt atcgttttac ctgaaccact ttggggtatc tcatctcttg ttctatcctt
     5581 tttaaattca ttgtctatat taggtataac caacttttcc ataaattttc ttataaaagt
     5641 tgattttcct gttcttacag gtccaactac tccaatatat atatccccct gagtcctttt
     5701 ggatatatct tcgtatatgt tattattcat attattcctc ctatacatct aatcttatta
     5761 aattatattt agagttaagg gttaatattc ttaaaataga aaaataaaga ccagcaatat
     5821 taatttaatt tacatataga aatttgtaaa tttcaatgta aattaaatta atattgctgg
     5881 tccaaatagt agtgtattat attaactgac tttagtagta ttttcatttg acttattatt
     5941 catctcaata agttctttac gtctttcttt tgtcatgttc atccatataa atgtaaacac
     6001 caatccaatt atggctacta tagtaagaac tatgaagtag tacctatatc cagttacacc
     6061 cggaaatgca tccaatatct tccctgctgc taaaggacaa agtacttctg gaagatatcc
     6121 taatgtagca actattccta tagcagttcc tgatattgat aatgaataat taccttcttc
     6181 aagtaacgaa tagtgtaaac cttgaattgc atacatagat acatatattg caacacatcc
     6241 aatcaatagt atataaatca tagatgattt tgttggtgta aatattacac ctaaaagacc
     6301 tgcaatcata agtataaatc cataagatat aatctttgat gaacctactt tatctccaag
     6361 taatccagaa gcagctgatg ctactggtct acaatactga gctaatattg agattgcaga
     6421 tgcaaacacc acagtagctc caaatgcttc agttgaataa ggcgtaaaat aataaaatga
     6481 catattagct gaatatgaac aaaacattat tattgttatt atccatgtat gaggcatttt
     6541 tattactgca aaaaaggctt ctttatcaaa ttttgctgat acctcttttt cattatcctt
     6601 taatttgaaa aatactaata ctccaactat tacatttatt gccgagtata atactataat
     6661 agctgtaaga ccagatttat cacttacttt tgatgaaata tacccaaata gtatcaatac
     6721 tagagataga tgaattgcat tggtaattcc acgaccactt tccataaatc caaaagcttt
     6781 tccttgttca ttttcagatg ctaacattct aacagctttt acaagagctg gccaaaaagt
     6841 aagtattgtt gttattcccc ataaggcatg gattaaaaac acaacaatat atggaggata
     6901 aagtgttaat acaagaccag atattcctgt aataattaaa gaacctgaaa gcaattttct
     6961 ggctgaaata cggtctgcta aaaaaccacc aaaaaggtat gataccattg caagtagacc
     7021 ataaatgctt cctaagcttc ccatttgtgt atttgtaaga ttaaatgctt gtgcaaaagt
     7081 atcataataa taattcttta aatatggtaa agcatatata aatgctcctg ctacaaagag
     7141 tattatcaag actttgaaat ttttccaaat gcttggttta tttacttcaa ctgacatact
     7201 tcctccttat gtaaatacct gtaaattttc atgataatta attattgctt attttattgt
     7261 ttattcaata tttcggaata tttattaata tattaggtta atctatcaat aatttatggt
     7321 gagttatgag ctaaaagtaa actcttttag ctcaccataa taattatatt tttatttata
     7381 ctaacgtcca gagttattag atatcttttt ttcatagttc ttccatagca ttaaacctgt
     7441 tattataaca aaaagacctc ctcctatcat ttcaatctca tagattatat tatcttttaa
     7501 agtctccata actggtgccg ttgagaaaag tatgccaact acagttacta caagtaccat
     7561 tctggcaata ttgatacaaa ctgaatcact ttttgcaatt gaatatggtc tttcttcatt
     7621 tggtcttgtc tttctaagtc taatataaga tgtaaaaagc aagacatatg gaaataatgc
     7681 tgttaatgct gtcatagtaa caagaacatt gtatatatta tctactgatg gtaataagtt
     7741 tgttcctaca agaagtagac tcactattat agcctgaact attactgcat ttgatggtat
     7801 attgtattta tttgtaacag tcaattgctt aggaaataca ccttctctta catttccaaa
     7861 aagcatcttt actggacctg ctatataaag tattaatgct cctagtactg ataaagttat
     7921 tccaaatgct actattctta taaaccaact tcctatacct aaatcttgtg caactttagc
     7981 taatgcatct agtatccctg tagacgctgc aattttatct ggaggaagta tcattgttat
     8041 tgctactgaa cctaagatat agattcctcc tattaaaaca gccacagtta atatagcttt
     8101 aggaaagttt ttcttagcat tatccatttc ggttatgaaa ttagcagtcg tttcagctcc
     8161 tgataatgca aatattatag cagatataga aactaatgaa ttggcatcta tttttggtat
     8221 tatattttgg attgtatata tcgatgctga tggagctttt ttgagaataa ctacagacat
     8281 aaatgccatc actataagta atatagcagg aattgtagaa cctaatgctc ctgtattcgt
     8341 aaaaaatttt ccaaatgcca tcccacgagt acttactaag gacaacagcc aaaatattat
     8401 gagtgaaagt gctaaaataa acatcttatt gtttgataaa tctggatttc caagcatata
     8461 tgcaatattt atagctaaaa atgtcaagaa agaagaatac caaaatatct ttgatgtcca
     8521 atttagccat gacaccataa atccccactt ctctccatag gcttgtttaa cccattctcc
     8581 aagtccacca tcttttggat atgttgcagc caactctgca catataagtg atgcaggaac
     8641 aaagaatata aaagcaaaca ataaccaaac tggtatagaa ccaagaccca atccaaaact
     8701 agaggcagta gatttagcta tccatctaat cccaaatagt gctgatatat tcataaatac
     8761 taaatcccaa aatccaattt tctttttcac tgtaattcct ccaatttcaa atttattcaa
     8821 actcatacac aatgtattga tttaaatatc aagtataagt tcttcaacac ttcttggata
     8881 attagttata attgtagcac cattcttacc tactattgct gtatctgagt gtctaaatcc
     8941 tccaactcca ggtatatata tccctggctc catagaaaat accattcctt cttctaatat
     9001 taattcattg tcaaatctta aatatggctc ttcatgttct gataaaccta aaccatgacc
     9061 aattctatga tttgaatata actctagacc atactttgca acaacagctc ttgctgcttc
     9121 atccaccatt cttgctggta taccagcttt tattgtatct aatccagctt gttgagcctc
     9181 tactgcaatc ttaaacactt ctttttgtct ttctgttggt ttacctataa taaaagttct
     9241 ttcattttct gctctatagt tttcatacca aacctgtcta ctatgtatta ctatatctcc
     9301 tctttgaagt atcctagtat tggaatctaa atgaggttgt gctgttctat caatccctga
     9361 acaagtccag tttgcaaatc ctatatatgt atcaggataa ttttcagaaa catattttag
     9421 taaagcatta tcccccgcaa cgtcaaactc caattcactc atcccaactc taacattttc
     9481 taaagaccct tttattccta tatcagatag gtaacctgct atcttcaaaa attctatttc
     9541 ttttgcatct tttacatatc tcatttcaaa tattttactt ccaacatctt tgatagcaaa
     9601 tgacttatca gttatatact ctgaaaaact tgcagatact atatcttttt ctattccaac
     9661 agttgttcct ttagggtatt tatttaaaat tatatctaag taatgtttat gtgatattcc
     9721 atgttctttc atttctggta tttcataata cacatataca ttatcaactt ttgcaacttc
     9781 tttagcatgt aattcttcta atccaggtac tattaattct attttatctt gagttactac
     9841 tgttacaatt ggtctagaat aagttattgc tttaaatcca ctaatataaa attgattgtc
     9901 tggttcaaat attaaagcaa tttcaatgct tttttcattc atgtactttc tcaatctttc
     9961 tagtctcata ttatttgaca taatgcacct ctttcttaat attcagataa tttattcaat
    10021 acaacaagga taaatatatt attttaaaat tcttaatatt tttagtactt gtcgtatttg
    10081 catgtaaatt aattttattt gtgattattt tatcataatc atgattattt tgtcaatcac
    10141 gattttattt ttaaaatttt tttatattct agtgatttta aattatctaa aatataacga
    10201 gtatacattg tattttggaa tattattctt gatttatggt aagtttaatg ataaaatcac
    10261 gattatataa actatattga ttatttgtag gtgaataaaa tgacaaataa agaattacaa
    10321 aagaaaagga tattgatgta tttcatagaa gctgctcaaa atattatgga aaacgaaggt
    10381 atagaaaata ttacacttcg taaggttgca gatatggctg gatataatag ttcaactcta
    10441 tataaatact ttaaaaattt agaccattta atatcatttg catctataaa atattttaaa
    10501 gattataact tgaatatttc aagatgtata gaaaatgtca atgatgaata taagaaatat
    10561 ataattatgt ggaaattatt ttgtaagcat tcttttgaca atgctcaagc tttttatcag
    10621 gtatttttta acttgtctag tgatgaactt agctatataa caaaacaata ttatgatatg
    10681 tttccagaag atttaggtat tcatgatagt gacatatctt taatgattac aggagaaagt
    10741 ataaaggaaa gaaataagat actattaaac aatcttgtag atactggata tataccagct
    10801 aaagatttag acataatcaa tgatataatt atttcttttt atcaaaatct attattctta
    10861 aaaaagaaca caggaaaaaa ttctggtgat gatgaattaa ctatgaaaat gatttcttct
    10921 attgaataca taataaaaca tgctaaataa aattaaaata tggctgtctc aaaatagaga
    10981 taagcctttt ttattgttga aataataaaa atatatttaa tttcatataa aaaaaagtgc
    11041 ctcgtaaact aaaaagttca ttttgagaca cccctaaaac aaatttatta tttattatac
    11101 aatagtttat atatcatcac ctaaaactac ttcttccatt tcatgctttt tacttctcat
    11161 cattagttct attccaactt ctttaggatt tgaaccttta tttattacag aatatatagc
    11221 attagttata ggcatatcta tatctaattt ttcagcaaca tcatgtgcta cttcagtagc
    11281 agttatgcct tctacaacca tctttacttc ttttaaagta tcttctaaac tcataccttt
    11341 acctatgagt ataccagctc ttctatttct actatgcata cttgtacaag taactattaa
    11401 atctccaata cctgaaagac ctgcaaaggt agacatatta gctcccatag caattccaag
    11461 tctgctcatt tcactaatac ctcttgtcat aagggcagct tttgcattat caccataccc
    11521 taatccgtca catatcccag ctccaaatgc tataatattt tttaatgctc ctccaagttc
    11581 tactccaact atgtctggat tcgtataaac tctaagttta gggctcataa ataaatcttg
    11641 tatcatctgt gctattttta aatctttaga agcaactact acggtagtag gaatatccct
    11701 tgctacttct tcagcatgag atggcccaga tagtattaca tatggatttt gtggtaattc
    11761 ttcttcacag acttgagata atctaagccc agtccctttt tcaagacctt tagctacatt
    11821 aacaataact tgtccttctt ttataaatgg ttttattttt tggcaagtac ttcttacagc
    11881 ctgtgatgga actgctaaaa ctattacctt acaatctttt atagtttttt ctatatcagt
    11941 acttatagtt atattgtttg gaaataatac tcctggtaaa tagtctgtat tttctttagt
    12001 tctgtttatt tttttagctt gttcctcttt tcgagtccac atgctcacat cattaccttt
    12061 ttttgcaagc cctaaagcta atgcacttcc ccagcttcca gtacctaata cacatacttt
    12121 ctccatgttt aacaccacct gagtataata tatttactat tctttctttt gaccaatttt
    12181 tctctctgtc ccatttaata atcgctttat atttgctctg tgattatata ttactgatgc
    12241 agttaagaac agagctacta ttaaaccagc tttatttttt accataatca taaatattgg
    12301 tgaacatcct attcctacaa cagaacccaa tgaaacatac tttgttatag ctaccaccaa
    12361 tataaatact gctagacaca ttagtgttat tactggattt acagctagca ttgcacctag
    12421 tgaagttgca acacctttac ctcctctaaa tcctaaaact gctggccaat tatgacctgc
    12481 tacaacacat ataactgcca gatatccagc taaaagagta tctattccaa ccaatcttgc
    12541 tgcaaattct gatattaaaa cagccattac acctttaaga acatcaccta aaaatgtcat
    12601 tgctcctgct ctttttccaa gtgtcctaag tacatttgtc gaacctgcat taccagaacc
    12661 ttgagtcctt atatcaaccc cagcaattcg ctttgcaact atgtatgagg tagatatatt
    12721 tcctaaaaga tatgcaacta cagcaataat tatataacta aatatctcca ctgaatttcc
    12781 cccctttagt tatattttaa gatatttatt ttttattttt ttgtctatac tcaaacttta
    12841 tggaagttcc ttcaaatcca aaattttctc ttatcttatt ctcaaggtat ctttgatatg
    12901 agaagtgagt taaatcctta tcatttataa acaatgtaat tttaggaggt ctaatgtcag
    12961 tttgagtccc atagtaaatt tttagtcttc tacctttgtc agatggtggt tgatttaaca
    13021 ttacagcttc tccaattact tcattcagtg ctgaagttga tattctctta gaatgttcat
    13081 ttgacacata ttcaactgta tctagtatct tgttcattct ttgattagtt tttgcagaaa
    13141 caaatacgat tggagcatac atcataaatg gaaatttttc ttttatatct tttgtgtaat
    13201 tactcatagt cttattatct ttctctatta aatcccattt attgattaca aatatgcatc
    13261 cttttccttc atcatgtgct atacctgcaa cctttgtatc ttgttctgtt acaccttcta
    13321 aggcatctat tactatcaat actacatctg ctctatctac agcactcata gctcttatta
    13381 cactgtattt ttctattgtc tcatatattt tactttttct tctaagccct gctgtatcta
    13441 ttagaagaaa tttctgtcca tttttttcaa aataagtatc aattgcgtct ctagtagttc
    13501 ctgcaattgg acttacaatt actctttctt ctccaagtat cttattgagt atagaacttt
    13561 ttcctgcatt tggtttacct gttatagcta ctcttattat atcttcttca tactctgtat
    13621 ctaatcctgc tggaaaattt tcaactatct catctaataa atctccaaat cccatactat
    13681 tggctcctga aactgcaaaa ggcgttccaa atcccaactc ataaaaatca tatatattat
    13741 caaattgact ttgactatca atcttattaa ccactaagat tactggtttt tttgtctttc
    13801 taagcaattg tgcaatttct ttatcagcag ctgttattcc agctttacca tcaactataa
    13861 aaagtattac atgtgacatg tccattgcaa gcatggcttg atttctcatt tgagataata
    13921 ttatatcttc gctatcaggt tcaattcctc ctgtatccac taatgtaaaa tatttatcta
    13981 accactctac ttcagcaaat attctatctc ttgtaactcc aggtgtattt tcaactattg
    14041 atatcctttt tcctgcaaat ttattaaata ttgtagactt accaacgttt ggtctcccaa
    14101 ctacagctac aactggccta cttatactca ttttatttct ccttccatct tttgttttct
    14161 attttaaaat tttatccaca aagtcttttc cttcattcaa acaagggata atttttattc
    14221 tcataagttc ttctaattga tttaattcaa tattatctaa gaaaatttct tcatctgctt
    14281 ttagcatact tcttgttata taaagagctt ctcctaattc tttatcttca agttgttctt
    14341 ttaaatcctt agctgttaaa agacctgata cagttattgt ctctccaaaa aacttgtttt
    14401 tgatttcata tacatttact tgaagatttt taaacttgtc catcatagca tctgccatac
    14461 tctgtataaa ttcatatgca gaatgaccag tagctattga tactttcttc tctcttttta
    14521 gaatattctc aggcaatata tttagatact cttcaatctc tgtttttaac ttacgtatca
    14581 tacctacacc atcttcaaac tgtaaaaaac cttcatattc ttcatatcca ggcaattcac
    14641 tattagatag tatataaaat tcatctgata aaaatgcaaa tcgagtacct aatttttcaa
    14701 gatatttctg ttgtatttga tgtatttgct ctatagtttt acttgcgctc ttatcattaa
    14761 atatatttaa ttctactaga ttttctctat gctttgtaat cccaactgga actatcgcaa
    14821 cactgtttac atatggataa agttgagcta gttcttttat tgttctatcc aattcctttc
    14881 catcatttat acctggacat aaaacaattt gacagttcat ttctatatga gcatctgcca
    14941 atctcttcat tataccatat aattttcctg caaatttatt gcttatcatt ttttgtctta
    15001 actctggatt agttgtatgc acagatatat ttataggact tattctatat tttataatat
    15061 tatttatatc ttcttcactc atatttgtaa gtgtgacaaa atttccttgt aaaaaagata
    15121 atctcgaatc atcatcttta aaataaagag tttctctcat accttctggc aattggtcta
    15181 taaaacaaaa catacactta tttctacagc ttttagcttg gtcaattatc ggatttgtaa
    15241 attcaacacc caaatcttca tcatagtctt tttctatctc gtatatgtag acttcgccat
    15301 cttttttttg tatttctaca tctaaatatt catcacttag taagaatcta tactctatta
    15361 tatcatgtat tggctgttca ttcacagata taagtaaatc tcctacctct atacctattt
    15421 cttctgcaat actatcttta taaaccttac ttataatatt atttactttt tttacattta
    15481 cttccaatat tttcaccact cttttgtatt catcaatgct tattataata tattttaata
    15541 tgcaatttat ttatttatta tattttatga tagcattttt aagcatttca tgtcataata
    15601 acacatttta attctaatag tcaatataag taaatagcta taaaatagat tagaacaaat
    15661 aatattcaaa tttaagtttc ttaaaaattt gaatattatt tgttcttaaa aaaattttat
    15721 aaatttaatg ttttactatg attaacgtcc catttgtcat atcatgaacc attccaccta
    15781 cacactttga catcaacaat gccttatttt gtaagtcttt ctcatcattt gcataaaata
    15841 ttggacaacc tccaccattt ttcatattct tatcaagtgt aactatagct agggtatatt
    15901 catttaaacc tatatccatt cccatcttat gcacttcctt tctttcctat tttataattt
    15961 tttagtgata agttttttcc tttagagctt gaaagtattg ggcatgactt tacagcttcc
    16021 attacttttt ccatattctt atctactggt aaataagcaa tcattatact ttcatcctct
    16081 ttatttcttc taggtaatgg tacgaatgaa ggttctccat aatctccata taaacctaac
    16141 cttgaatata tattgtatac tattgcttgt ctttgaccgg agtcaaataa tattcctgca
    16201 ttagcataat ccttattttt aggaattatt tgtattccta cacctctatt aatatatact
    16261 tctctatcac tttttagacc tatatttgtt atacctttta aatctcccac ctttagtata
    16321 gattcgtcca caaaactaat ttcagcaggc attacatcag ctatgtctcc aatacttttt
    16381 ctagtaagaa gtttttttaa tataaaggct aacactaatc cacaaattga acttattacc
    16441 aaactcatcc cttcacttat tttcgtttct gcaagtatta cataatacaa tcctattgtt
    16501 aaaaaagatg taactataca catataattt cttacttcat aggttctagc tatctcttct
    16561 atgaatgcag cacctctctt tacaagttga acttcctcta aattttgcaa tgtatttctt
    16621 ctatttgctc tcacttgtct aaattgttct gctgctagag ataaaaatgt tatggatgta
    16681 taagaacgat ttataagagc tggaaccaat aatgctccta atgaagaagc gacgaatgct
    16741 aatataagtt gagatagtaa aacatttggt tgcgttggat actgtttttg gtctaaattc
    16801 aacattataa ttctagatgt tataccaata ataacagcaa caaaaaatac tctatccatt
    16861 aaatcactcc taaaatattt aatttgaaat tttacatgta aatctacaaa gtctttctag
    16921 aaaaatcact ctgctttcct tttgatgttt ctatgatagg tgtacttttc actacatcta
    16981 tcatgacatc tatatctttg agtattggca tatatggtat aaccacagta cttttttcta
    17041 aattggtctt agatatagca acaatatcaa cctcatctac atctctatct atccccatat
    17101 gaataaatag attatgtatt atagcttgtc tttgccctat atcatttact attccaaatg
    17161 cacctaaatc tcttggtata acttctatgg ccagacattc atttctatac ttttctcttg
    17221 tactttctag acctatatta gttattatta catcatttac cattaaaata ggtccttcaa
    17281 aacttatttt tgcaggaact atatctgcta tatctgctat actgtttctt cttaaaaatc
    17341 ttctaaaaat caagccaaca atagctcctg acacaatagc taaaattgtg caatgaaaaa
    17401 agctaaggtc atactttctt gcaaaaacga tataaactat cgaagatact agtgcagaaa
    17461 aaagacttat ataacttcta gattcatacg tagatgctat ttcttcaaca taagcatctc
    17521 cttttggtac taattcttca ttatcaatat tctttagtgt tattctttct tgttccgcaa
    17581 gaccttgaaa ctgttgtatt gctactgcaa aaaatgtcaa ggctgcaaac tccttatcta
    17641 taagtgcagg taatgctata gcacctaacg aagctgaaag cccagatata attatctgtt
    17701 ctaaataatc ttgtggtctt gatgggtatt gtttgtctgt aactcttaaa actaatcctc
    17761 tacataatat tcctacaact aatgcaacta taaaagagtg tctaaataaa tttgcattca
    17821 ataaactttc ttccattttc tcagctcctt ctcttatata gtttgtgcaa aaaacaaaaa
    17881 aaattccctt ctatttaagg aaattttttt gtaatattgt aactataaat tcgctaccaa
    17941 tgccaagttc actttttact tttatacatc catttagtga tttgactata tgctttgtta
    18001 tagccaatcc taaacccgtt cctcctacat ccctacttct tgctttatct accctataaa
    18061 atcgttcaaa tattcgttct acatcttctt ttggtatccc tacaccattg tctataactt
    18121 taattacgat tttttcttca tcttttaaca ctacaacttg tacaatccca ttatcttggg
    18181 tatactttat agcattatca actaggttta agaacacttg ctttatatag tctttattgg
    18241 atagtatact tattgattta tcatcacatt tatataaaac ttctatattt ttttcactag
    18301 ctgaatgttg agttatttca tatacctctt taaatacatc atatactaca acattttcca
    18361 taagaagagt atctttattt tctacaaatg ataacagcaa tatatcatca atcaaccttt
    18421 ttaatctatc tgattcgctt tctattattt ctaagaatct atttctagta ttcacatcta
    18481 tattttcatt tgactttagt gtttcaacaa acccacttat agatgtaagt ggtgttttaa
    18541 gttcatgact tacatttgca acaaaatcac ttctcatatt ttcaagttta acttttttag
    18601 taatatcttc tatatttact atcgaaccta ttattacgtt gttagtattc tgaagataaa
    18661 ctgggtctac ctttatctta tatacgatat catctttagt agtaatatta gcagtttcat
    18721 tatctttaga accaataaat tgaagtattt gttttaatat ttcttcttct tttattacaa
    18781 cattaatatt atgaccttca actaattgat ttggttcact ctttatcatt tcttttgctt
    18841 catcatttat cagaagtata tttccattta catctatagc caaaattcca tgagatatac
    18901 tctttaatat agatcttaac tgtaaatgtt tatactgtac ttcttctaag gttttatcca
    18961 tattctttat catatgatta aaatttctag ctaattcacc aagttctcct ttggctgata
    19021 tgttaagcct agcatgatat tcattattac ttactttttt tgagacgtat ataaactctt
    19081 ttaaatattt ccttaatgtt attgtatatc ttacagctaa tataacaatg gctatggaca
    19141 acataacaat aaaaaaatat atactattta aatcttccac aagataacac tcctaaaata
    19201 taaccttttt aatctatttt ataccctaca cctcttatag tctctatata tttttccgat
    19261 ttatcttcat cttctatttt ttttcttaaa tatcttatat gaacatctac tgttctagtt
    19321 tctccatagt attcataccc ccatatttta tccaaaagat aatttcttga aagtactttt
    19381 cctttatttt gaattaataa ttttaacaat tcaaattcct taagagttaa ctctatcttt
    19441 tcagagcctt tggtaacttc atgttttgat aaatctagtt ttaaattgcc tgtagtaagt
    19501 atattgtttt cttcacctaa gctagtcaaa ttatatctcc ttaaaacaga actaatccta
    19561 gctaacaact cttttatact aaatggctta gttatatagt catcagcacc tatttcaagc
    19621 ccttcaacct tgtcattttc catattttta gcagtaagca ttataacagg aatatttttt
    19681 aagtccttat cacttcttat ttttctcaaa acttctattc cacttatatt tggtaacatc
    19741 caatctaaaa gtattaaatc tggtttaatt tccttagcct ttataaatcc gtcaaatcca
    19801 tcatatgaat aactaacttc gtaatttgac acctccaaat tgaattttaa tagctcaact
    19861 atatgcattt catcgtctat aacaagcact ttagtattca ttaaaaagct cctctctatt
    19921 ttatctgtag taccgttcct attcgttctg atgccttatt atgttttcta taagagtgga
    19981 atttatctgc tctacaacta gtgcataaat ttaaatttat tatattttct tttttaactc
    20041 cactacatct aagcatatac tcattaattt tccataaatc taaatagtaa cttccctctt
    20101 ttattatata gaacttttca tcattatttg taagaattgt gttaaatttt tctactaaat
    20161 cttcagacac ttcatagcaa catggtccaa ttgatggccc aattatacat actaaatctt
    20221 caggaactgt tttatagcat tcagacatta attttattgt tttatgacct atattactaa
    20281 aagtccctct ccaacctgca tgagccagtc ctatggcttt atttttagca tctataattg
    20341 atataggtac acagtctgct gtaaacagta aaagtggtac tttttccaag tttgttataa
    20401 gtgcatctcc atctcttctt ttccctatag tatctttatt gactatatta actatatctg
    20461 aatgtatttg agaattacta gttatatttt caaatgtcaa atcacattct ttacatactt
    20521 ttaacatatc ttctctattt tttgcatcta tattcttaca tgttataact agatttacag
    20581 aatttaattt ttcatcaagt tcttcacaat taccacttaa attaacatac tctttaatat
    20641 cttgtacatt ttttatattt atataatctt tcatcatttg tctccttttt ctatcagtat
    20701 acttttaagt tcatttacaa aagtatttat atctttaaat tgtctataca cagaagcaaa
    20761 tcttacatag gcaacctcat ctaactcttt caatttatcc attaccatct caccgatttt
    20821 tttggtttca atttcttcta taaaacattt atttatttca ttttcaatat gtacaacaat
    20881 actttcaatc tcttcaatag aaacaggtct tttttcacat gcttttagca atccatattt
    20941 tatcttttct ctatcaaaat attctctact attatccttt ttaatgacca ttaaaggagt
    21001 tgtttctatt ttctcatacg tagtaaacct ctttttacat gattcacact cacgtcttct
    21061 tctgatagat tttaaatctg tatgtctaga atctattacc tttgattctt tataattaca
    21121 ataaggacat tgcatctata ttcctccttt gtttaactat atctcaaact catctaagct
    21181 taattctgaa cctatattta ctattattgt atcacaacct attttaagta tatcattcca
    21241 aaatataact tcttcatccc ctcctcttga aaaaaaactt ctacttccat ctccaaaaat
    21301 ggtaaatgag actaccttac cttcgtgaat atccatgtct atgtctatta taaaccccat
    21361 cctttttcca ttttttacat ttataatttc tttttccatt atatcagaaa ctctaatcat
    21421 attgtactca ccctttcttt aaaaataaat atgtatcgaa gtaaaaaaaa atgacctata
    21481 taggtcattt aaattaaaat aatgtttaca gtccatggat tacttataca tattttctca
    21541 tattttttaa agcatttttt tcaattcttg atacttgtgc ttgagatata ccgatttcat
    21601 cagcaacttc tatctgagtt cttcctttgt aaaaccttaa atctaatacc aacttttctc
    21661 tactatttaa tttcttaata gcttctttaa gtgatatttc ttgcaaccaa ttttcatcag
    21721 tatctttttt atcttgaacc tgatccataa caaatattgc atctccattg tcttgatata
    21781 ctgggtcaaa cagagatatt gggtcttgta tggcatcaag tgccattaca actgattcta
    21841 cttctaattc taattccttt gctatttctg atactgtagg ttctttagaa tttgttctaa
    21901 ttaatctttc tctaacttgt agtgccttat atgctatatc ttttaatgac ctacttactc
    21961 taattgggtt attgtctctt agatatcttc taatttctcc tataatcata ggaacagcat
    22021 aagttgaaaa ccttacattt tggcttaaat caaagttatc tatagctttt ataagaccaa
    22081 tacaacctat ttgaaataaa tcgtctatat tttctcctct gttgttaaac ttttttatta
    22141 cactcaagac taatcttaaa tttcctctta caaactgttg tctggcttct tcatcaccat
    22201 tttttatttg taataaaagt tccttcattt gcttattttt aagaactgga agttctgatg
    22261 tattaacacc acatatttca accttattaa cttgcatata ttttcagtcc tctctcaaaa
    22321 gtattcatct attgaaatta tttacatttc caattttttt atacttttta agaagaatta
    22381 atttaacatt atacaaattt tttcatttct ttttgcaacc ttgagattat tttcttttca
    22441 agtctagata tgtaggactg tgatattcca agcattccag caacttcttt ttgtgttttt
    22501 tcaatacctt tatcaataag accaaatctt aattccataa tttgcttttc tctgtcactt
    22561 aatctgtcaa gtgccataac caacaaatct ctatctattt cttcttcaat tattttgtat
    22621 atttcatcat tttctgtacc caatacatca gataataaaa gttcatttcc atctaaatct
    22681 atatttaaag gttcatcaaa tgatacttct gttttctttt tattattttt tcttaagtac
    22741 attaatattt cattttctat acatctcgaa gcataagttg ccaattttat atttttattc
    22801 agtttaaaag tattaactgc ttttatcaaa cctatagttc ctattgaaat caaatcttct
    22861 acatctatac ctgtattttc aaattttctc gatatgtaga ctactaatct aagatttctt
    22921 tctattaaaa ttgactttac actttcatct gtttctagct tttgtaaaag ctccatttct
    22981 tcttctggtt tcaatggagg aggaagtata tttgcccctc ccatatagta tattcccttt
    23041 ggctttatta tttttaattt aatacctaac atagtaataa agcttattat tctttctttc
    23101 aatcgtaaca ttccttgtcc cccttaaatt ttcacctaaa atccaggcaa tatatttgga
    23161 ttcattattg ctccatattc tctatcatta aaatttgaaa taccaagtat tatatttcct
    23221 atcttttgct catctacttc tatatagtca gcttttaagc ctattattat gcttgtctta
    23281 ttacttccag catgtttata aggtattatt ctaactctac ctgaagtttt atcatctaaa
    23341 cttcctataa tttcttgtgc tttcttcata tctatatctt cataattaat atttaatagt
    23401 ttttctggaa taaatccttc taacacacta gacttaacta ttattacatc actttgactt
    23461 attgggtctt ttaacaaatt accactatct actaaagctt tacagcaaaa acttttttct
    23521 agtaagttta tatttatagt ctttgtaagc tccttaatgt acttaagcat tttcaaatct
    23581 ttataaatgt attttaataa ttcacaactc acataagtac atgcaattaa gaaagatatt
    23641 tttaagtgac ttattcctgt aaaataaatt ataaaataag tgcttccaga aataaaaatg
    23701 tttactagat aaaaaactaa cgctacatga acataatctt ttttgctctt gcttctaaat
    23761 gaaattaaag ttattatgct catgattatt attttaaatg gtacagtaaa tagtatattc
    23821 attgatggat acaaataagc aacagaatag gcacttccta aactagcccc caataactta
    23881 ttttttttag gattatatct tttagtaaga atagaagtac aacttataat aatataattt
    23941 attactaggt tttctataac atagtactca atatacacca tgtatccccc cttaaaacct
    24001 ttaaacatat tataagatag tatgtacaaa attttatgtc acttttgatg gtcgaatcca
    24061 aaacaaattc tatttaaaaa gaacaatgat atatgtctta tactttatgt cgtcaaaaga
    24121 tattttttta atgccaaaca gatagacttt tttgtagaat ccaatatatc taggtagaag
    24181 ttattcgctt tacttttggt atatttataa aaaaaagact acataattta ataatatatc
    24241 aagtaaatct tttacttata taaaatcaaa ttatatagtc tgtaaattca aatataaatt
    24301 attgtgctag taatccattt gcatattttg cagattcatt tagtgcttgt tctggagtta
    24361 tgtctccagc tatcatcatt tgaagttgtt tccataaata ttcatatatc tctactccat
    24421 gaggtccaac tgtcaatggt ctatacatat gtttatctct tgtcaccatt tcttcaaatc
    24481 ttgggtctcc ttggtatttt tcacctttag caactggagc tctaggtatt tctttgtgga
    24541 aagcttccat tgattctttg cttaacatat actcaagaag tttaaatcca agttctttat
    24601 ttttacatgc tgacattaat gttaaatcat ctactgaagc aaaacttcca actgtcttat
    24661 cttctaatga tgttacatat ccccagttta aatccgggaa tgatttatca aatgtacttg
    24721 tagcagcaga ggatagcatt atagtaaatg cagcttttcc tggtccaaat acactttcaa
    24781 tcatttcatt actatctttt gataaagcat cttctggaat atactttttc atatcattta
    24841 taaagtttgc agctttcaag ccttctgcat tattaaattt aactgatttt aaatcatcat
    24901 tatatatatc tccaccagct tgccataaat acggatacca gttccagttt aagcttccaa
    24961 atactttaga accccaacct tgtgcaagac cccactggtc gatttttcca tctccatcag
    25021 tatcttttgt tgctttttca cagattcttt taaaatcatc ccatgttttt ggaggttttt
    25081 ctcctaattt ttctagtata tctttattgt agtacaatac gcctggatta gcagcttcta
    25141 taggtaaacc ataaatacca cccatcattt ttgcatcatc taagtataga taattatctg
    25201 aagttcctga tttttctaaa taaggtgtta aatcttctac tgcacccatt tctataaatt
    25261 gagggaacat ctctgcatac atgtatccta tatctggacc ttcacctgca cttattgctg
    25321 ttgcatattt ttctgcgtag ttatcccatg gtactatctg aaattcaatt tcacagttat
    25381 tttctttttc aaatttttca aatatcggct tccatgcatc tgcatcatgg tctgttaatg
    25441 gtgcagacca tacaactaat ttttctttac cctctttgcc tttagaatct tctttttttg
    25501 aacatcctgc aagaattgat actgatagaa tccctaccat aactaaagaa agaacctttt
    25561 ttagtttaat catgcttaat cccctttcag actttaattt gtctttttcc tcaaatgttt
    25621 acatttaatt cagatttaat attaacaaat aagactactc ttgtctaatc ataaaaaact
    25681 atactaactc ctataactat attttttttc tatacctatt agatttaatt catactaaaa
    25741 acttactttt taaattttat caatatttaa aaatattttt aagtctagcc acaaactaac
    25801 tatctaaatt gaacaaaata aaaagaagtc tattgacttc tttataagct tacatattat
    25861 cttcttcttc ttaggaatgt aggtatctcc atgctctcat catcatcttc ttcaattata
    25921 cttgcctttt taggaggctc tattttttcc tcagcaatta cttcttcttc aatttctttt
    25981 acagcttgtt ttactgttgt atttaatgaa gaacgtatgc ttgatttagg tttagtatct
    26041 aaatctaagg caccattttg taatccttca aatcctgttg cgattactgt aatcattatt
    26101 tcatcaccta aatcttcttt tatagaagct ccaaatataa catttgcttc tgggtcacag
    26161 gattccataa ctaaagttga tgcctcatta acttcaaata atcctaagtt aggaccacct
    26221 gtaacattaa gaagtactcc tttagctcct tgtattgatg tttctagaag tggagattga
    26281 atagcttgtc ttgcagcatc aattgctcta gtttcaccac tagcactacc tatacccata
    26341 tgagcaagtc ccttgtcttt cattatagta gtaacatctg caaagtccaa gtttattaac
    26401 ccttcaactg ctataaggtc tgaaattgat tgtatacctt gttttaatac atcatctgca
    26461 actgcaaaag catctaacat tgatgtattt ttttgtacta tctgtaaaag tctatcattt
    26521 ggtatcgtta taagagtatc tacttttgat ttaagttctg ctattccacc ttcagcgttc
    26581 ttcattctta tcttaccttc aaatgcaaaa ggtttggtaa ctacacctac tgtaagtatc
    26641 cccatttctt ttgccaagcc tgcaaccact ggagcagctc ctgtaccagt accaccgccc
    26701 attccggctg ttacaaagac catatcagct ccttgaagta actttactat ttcatcttta
    26761 ctctcctcag cagctctttt ccctacttct ggatttgctc ctgctccaag tcctctagtt
    26821 aatttttctc ctatttgaac tttatattca gcttttgatg tatataaagc ttgtttatct
    26881 gtatttactg atataaattc aacgcctttt agttgagctt ctaccattct attgacagca
    26941 ttgtttccac cgccacctac acctattact ttaatttgtg cacattcttc taattctacg
    27001 tcaaagttta gcatcataaa cccccttctc tcatgattca aagttatcgt atgtattaat
    27061 tatatcattt tcttctttta ttttgttaac ctttaagtaa catattccac aaaaacgtaa
    27121 attttccttt ttaaatttct tacttatatt gtcaaatatt actctttaag tataaaataa
    27181 tttacgtata tattaaatgc tctttaattt ttacgttttt tgttcataat attactaata
    27241 atatatcgtc taattataga taagttatta aacaatctca taccaaaaac tagtacagca
    27301 acatagtaaa gagaaactcc taacttttct cctatataag tcaataatat agccaatata
    27361 gcattaccta tgaaaccagt aataaaaatt acattatcat agttatcttc aaaattggat
    27421 tttatcgctc caaaaataga atccattatt gcaagaatag ctactgacat atatatggaa
    27481 taatccatag aatatgtaaa tggaatatag taacctacta ctacaccaaa tgctaatcct
    27541 cctaaaaccc acaacataaa tcttcacctc tttattttac tttgaaacac aactacatta
    27601 caatcctttt tattaatttt ttttactttt tctttatttt tttgtatttt tcgttttatt
    27661 ttgcaaataa attcacttta ttcccattat tttcctgaac ttttatatta attccatata
    27721 tatccttaag agatttagca tatgattctg gagagtttat agctgcactc aataaatcta
    27781 tatctcctat agcttttatt ataaatggtt gtccatatgt agtatcattt actgtaatag
    27841 tagctcctga acattttatc ttacttgttg ctagtactct ttccccattt atagaaattg
    27901 ctcttgctcc agcctttttt aaatcattta aaactctcaa aatatctata tcatgtataa
    27961 tcaaatcatt tggattctgt ccgtctttta actctctctc actatccctc atagtaatag
    28021 ttatgcctgg ccctgtaaca gttgaataac cacttaattc tttaacttct tcaagctctt
    28081 tttgcataag tgtttggata gattcatttc cattcttttt tgcttcttta tattcattca
    28141 aagttttttt gttgttttta agagtttctt ttaaattttt tatttctttt tttgtatttt
    28201 ctacctcact ttcaatactt tcagtttgac ctaatgttat gtatactcta tttgggtcta
    28261 gtgattttat ataagttgat atgaaaatgc ctaatatgaa tgctcctaat ataactattt
    28321 tctttttcat actattattt cccctctgct ttgtttatat cactatgacc atttatttct
    28381 atattgtctt ctttggtcac ttttactttg gcattataat atgtttctat ttcccagaca
    28441 ataccatact ttatattaag tgccgaagcc aatgtatcag aatcaccaat tgcttttatt
    28501 ataattggtt cttttatgac agtgtcattc atacaaagct tgtcttcttt taaatacata
    28561 tatgtattaa aaactattct ctggtcgttt atagatatgg cacttgcttg tgctgaattt
    28621 aatttattta tcattgacaa taataaatca taattatata gtatactatc atttttatca
    28681 gagtttccta agtcttttgt agaatttatc tcaattttta tgcctgttcc ttcaacatct
    28741 gtatatccag ctaatgtttc atatttttct atttctgact taagtatgtc ttgttctcct
    28801 ttattgtttt tgcctctata agtctctata tctttcttca cactattaac ttctgattgt
    28861 agattctctt tttctgtttt taaagattta agttcttctg taagttgctc tcctctttta
    28921 aaagttgtca tccctctatt ttctatatta ataattttaa gttgtaaact aacaagaaca
    28981 cccaatacaa cacttcctat gattatactt ttatttataa atcttttttt caatatatca
    29041 cttccttaca attgtctatc cttctggact atacactgcc aactttccat agttaagatt
    29101 tatagtccct ccattagtat ttttattttg caggtcaatc agaattttac ttaccctaga
    29161 tatattataa tctaagttat catcattaga aagtaagatt tttatattac ttcttgtaag
    29221 catttctata ttgctttctt tttttaagtt tacataattt atttttctat aaattccttc
    29281 ttcttttaaa taatttaaag ttttaaatac attctcctta gttttataat ttttaaattt
    29341 tatagattta ttatcatcaa cgctataatc tacatctact atcaagtctt ttttactttc
    29401 attagaacct cttagttctt ccaataaatt tcctttttta tctatataac aataattatc
    29461 ttcatctttt aatactgcaa atatttcttt ttcttttaaa cttataatta tcttattggg
    29521 aagttttctt tttatttcca cattttctat atatggattt tttataagtt tatttttcat
    29581 atctttaaaa ttgtatgcaa aaatattttc atttagattt atatttagtt ctttcattat
    29641 atttgatttg gtcaccctct tatttccaat tacatctatt ttctttacat caaataaatc
    29701 actttttaca attatacata tacaaaaaga taacataagt ataaaaacta aaactatcat
    29761 gacattattc gtatttaact ttcttctttt tttcaaatat aatcaacccc tttggttaat
    29821 aaatacacct ttagagcttg tttttcctac aaaaaaatta taatatttaa agtcaaattt
    29881 ttgcatttta ttacataaca tccaaataag gtattaaatt ttataaataa atcaatattt
    29941 catcaaaata aacatagtat actacatact atttatatat ttaaatctgt atacatacaa
    30001 ataaacccct aatttataat aatttagaaa atctaatata ttataaatta ggggtaaata
    30061 tttacttatc ttttctttat tcctattacc ttagcttgac cttctatact tggagtcgtt
    30121 tcttttttta tttcttttac ttccttaata actttttcct tttttgtttt tttagaagtt
    30181 gacttttgtg ttgaattgta tactttcatt atctcatcat atataaggtc tatggcttca
    30241 ggcttaccta tagttttact tgcatttgcc atatcaacta aaagttctct atctcctaaa
    30301 agtttaaata ctgctgtatt taggctctct ggagttagat ttttttctaa aatagcaatt
    30361 cctgcccctt gtttttctat actcttggca ttatactctt gatgattttc agcagtataa
    30421 gctttaggta ttattataga tggctttcca agagccgtta tctcagctaa tgaaattgct
    30481 cctgcgctac ctataaccaa atcacttgct gcaagtgcat ttgccatatc ttctaagtaa
    30541 ggcacaactt tttggtatgg tttaagattt atatcgctaa tacttcccat aaattcatca
    30601 taatagcttt tgcctgtagc aaatataaaa gctatatctt catttaccat attctttatt
    30661 acaagtctca ttgcatcatt tatttttctt gaacctccac taccaccata gcaaagtacc
    30721 attctttttt cgtcacttat tgatagattc tttcttgcaa tattttttct tgataataaa
    30781 atttcttttc taactggatt tcctgtaaaa actaatttat cttctgctgc ctctggaaat
    30841 cttttatgcg aatcttcaaa acttgtaagt acctttgtaa ctgttttaga taaaatctta
    30901 ttagttacac caggaaaaga attttgctca tgtattatgg ctggtatttt ccccatagat
    30961 gcattaaata aaacaggtcc actaacatat ccaccagttc ctataactat atctggttta
    31021 aatttcttaa caatctttct tgattgctcc aaacctttaa aaagcttaaa aactcttttt
    31081 acattatcga aatctatttt tcgcttaaaa ccttgaactg ttacagtctt taattcaaat
    31141 ccatacttag gaactatttc tgactctatt cccttttccg tcccaacaaa aattatctca
    31201 gcgtctggat gctcatctct tattttgttt gcaatagcta tagctggata cacgtgccct
    31261 ccagttccac cgcctgataa taaaactttc atcaaatcat catctcctgt taattaattt
    31321 tgacatgctt tgagatgttt aacactattc ctacagctcc cataaagatg gtgagtgaag
    31381 ttcctccata acttataaat ggtagtgcta cacctgtagc tggcattgat gatgtcgcaa
    31441 ctgctatatt taaagctgct tgtattccaa tttgtgcacc tatacctata acaaccatac
    31501 aagcaaatac attagaagtc ttcaaagcta ttcttacaca tctgtagact aaaactacaa
    31561 atagcataat aactattata caaccaatca atcctaactc ttctcctatt atagcaaata
    31621 taaagtcatt ttgtggctct ggtatataaa aatacttttg tttactcttt cctaagccta
    31681 gaccaaacaa tccacctgaa cctagtgcat atagaccttg tattacctga taccccttac
    31741 caagagggtc ttgaaaagga tctaaaaatg atgtaacacg actaagtcta tagggttcag
    31801 caattactaa tgcagcaaat aaagcagccc cactccctat catagcaaat acaattttca
    31861 tatccattcc agcaacaaat atcattacaa aagtaactag aattacagta cctgctgttg
    31921 acaaatttgg ctgaagtatt atcaacgcaa aaaatatccc tggaactacg agaagtggta
    31981 caacaccttt tgtcaaagat tttattttat cataattttt ttcaattaat tttgcagtta
    32041 atattattgt agcaaacttt gcgatttctg caggttgaaa tgttaaagct cctattccca
    32101 accatctttt tgctccgttt gcttctatcc ctaaaggggt caaaactaac aataataata
    32161 ctactgctat agctccaatt gctgtagcat ttttcttcca aaaactataa tctattcttg
    32221 atgtaataat cataactata aatccaagta cagcatatat tacattcttt tttagaaaat
    32281 agtacgcatc attgtgttta aatgaagatt gtataaaact tgcactaaac accataacta
    32341 ttcctacaaa tactaaaagc atagtagtgt aaaacactac tccatcaaat tcagtcttca
    32401 tccttatgtc aatttgtttt ttcaaatttt ctttaggcat ccaactaatc tacctccatt
    32461 cttccattta agtaagttta gaggtaagag attattttaa attgtttaca ttatctttga
    32521 agtcttttcc tcttacttca aaactttcat acatatccca acttgcacaa gctggtgaaa
    32581 gaagcactat atctccactt tttgaaattt cataagaagt cttaactgct tcttccatat
    32641 cttttactat atgtatgtca ttaaatcctt tatttttagc acaattttct atatttgatg
    32701 cagtttctcc taataagacc aatgatttaa catatgactt tgcagtttca agaagttcat
    32761 caaaattact tcccttatcc atcccccctg ctataagtat tataggtcta tcataagact
    32821 gtatagcttt tatagtggaa tctggattag ttccctttga atcatttaca tatattacat
    32881 tgtctaaatt tcttacaaac tcttgtctgt gttccactcc tttaaatgtc ataagtacat
    32941 cctttataac ttctaaatca attttgcaga catatgctat tgctatagct gccatacaat
    33001 tttctaagtt atgacctcca ggtaaactta catcatcttt atttagaaat ttgattttat
    33061 catctatatc tattattatg tcattatttt ctaaatatac tcctccattt acttttttag
    33121 ttcttgaaaa gtaaattact ttggcattgc atttatcagc taaagatttt actacctcat
    33181 catcatagtt taacacacag aaatcctcaa cagtttggtt cataaatata tttgcctttg
    33241 cttctatgta tttttccata gtatggtgtc tatttaagtg gtcctctgta atatttaata
    33301 ttgcgcttac tttaggtcta aattcatcta tactctcaag ctgaaaactg cttagttcag
    33361 ttactaacac agactcttca cttgaagttt ctatagtatc tattacagga tttcctatat
    33421 taccaactac atatgtatct cgttttgctc ttgaaaaaat ttctcctacc aaactagttg
    33481 tagttgtctt tccatttgta cctgtaatcc ctataaaaat tggattattc gatagtttaa
    33541 aagctaattc tacctcacca attatatact tgcctgaatt ttttaatttc aatatgaatg
    33601 gtaggtctag tggtacacct ggagacacta caaccatatc tatatcatct acatcttctg
    33661 ggtgatgtcc taatatgtat tttatgtcat ttatagattt caattcatca agtatatttc
    33721 tcaatttatt ttcatctttt atatcattta cgattatact tgcacccaat ttatctaaat
    33781 gtttaattgt agatatacca gtttttgcaa gtcctactaa taaaactttt tttcctttta
    33841 agtccatttt tcattccccc tagccctaaa atactgctat tatacttatc caagctaaaa
    33901 caactgttat aatccaaaaa ataaatacaa ctcttgtttc tggccaacca cactgttcaa
    33961 agtggtggtg tattggagcc atcttaaaga atctctttct tgttgcctta aaatatccaa
    34021 cttgaatcaa aactgataga gcctccgcaa aataaattcc tccaattata ggtattataa
    34081 gcactgagtt agttagaact gaaaatgcta caactgcacc tcctaatgcc attgaacctg
    34141 tgtcgcccat aaacactcta gccggatatg aattaaatcc taagaaacct aaacatgctc
    34201 caacagttgc tgctgctaaa actgcgactt ctgtatttcc agcaatagaa cttgcaaata
    34261 gcataaagaa tacagaaact attaatgtta ctcctgatgc taatccatct aatccatcag
    34321 tcaagtttac agcatttact attgctacga ttataaacat cattattggt atatataata
    34381 ttccaacatt tatcacaaaa tctgtaaatg gtatcataag ttgtgaagca cttgatgatg
    34441 atgtatactg ataaaaagca acataaaaag ataatgcaac ttgaaggatt attttttgat
    34501 atgcttttag tccaagtgat ctttttagct ttatttttat aaaatcatct aaaaagccaa
    34561 taaaaccaaa acctgcaata catataagac ctactgccat atcatgacta acttttactc
    34621 ttgtaagtcc agttattaga attgcaacaa tcattataat tccacccata gtaggtgttc
    34681 cgtttttagc tagatgtgtt tggggtccat catctctaac tgtctgacca aatttaaatt
    34741 ttctaagcat tggtataaat attggtccta ttataattac tattaaaaac gcaatcaatg
    34801 cggtgtacgt aagctctgtt attcctaaca tcataaaact cctctccctt gtcaattaac
    34861 tcattagttt aagcttattt atttagatat tcgactattt tttctaatct catgcctctt
    34921 gagcctttta ctaaaatagt gtctcctgtt tttactaatt catttaactt gttaaacaca
    34981 tcttctctat tctcaaagtg atatatattg gctgaattga atcctaattg ttttgcttct
    35041 tctccaataa acactgaatt ttcacctata gttattataa tatcagtatt attcatagat
    35101 gattttccaa ctaatctatg accatactct gaaatttcac ccatttctag tatatctcca
    35161 agtatcgcta cccttctacc tttatatctt ccaagtatct ttaaagcagc atccatagaa
    35221 tctggactag cattatatga atcattaatt attgttaatt tttcattttt tattatgtct
    35281 agtctcattt ttgtcccttt aaaatttttt aaaccttttt ttatataatc taaagaaata
    35341 tttagacaca aaccaactaa tatagcagac attgcattat atatattatg ttctcctatt
    35401 gttggtataa atatttcctc ttttttccca tttattacac atataaatgt taaactatcc
    35461 tcttccatag tataactttc acaataaata tcattgtttt tattaaagcc aaatgttttt
    35521 aacttatata catgttcttt ttcttttaat gtagataaaa acttatcatc tccatttaca
    35581 attaaagtat ttgtttcatc aaagtttgaa gtaatctcca tctttgcttt caaaataccc
    35641 tcttgtgaac ccagtttttc tacatgtgat aatcctatat tcgatatgac tgcaatttta
    35701 ggatttacta tatccactaa atattttatt tcattgaatc cagacatacc catctctatt
    35761 acagcacact catgttcttt atttagatta aataaagtca atggaactcc aaaatgatta
    35821 tttaagtttt tttcattttt tagtatattt aactttgcag atatggcaga gtaaatcata
    35881 tctctagtag tagtttttcc cacactacct gtaacaccta taaatggaat atcaaatttt
    35941 tctttatagt attttgaaat atctcctaga gccaaagatg tatcttttac ctctattaaa
    36001 tttatatccg aactttctaa ttttattcca ttactttcat tttttataaa agttttacat
    36061 ccactatcat atgcaggctt cataaatgta tgcccatcta aattttctcc aactatggct
    36121 acaaatgcat tttgggcact tgcctccctg ctatctataa ctatatcact tacacagtca
    36181 ttataatctc catatattaa cttcccatta gttgcaagta ctaactcttt tattgttaag
    36241 cactccatta ttaaactcct cctttaaaac cttagctata ttctcttagc aacaaaaggc
    36301 aatgtatacc gttatatata cattgcctac gataactctt aactttataa ttatatacta
    36361 tatttctgtt tttttgaaga tgtttatggg tatatgttaa ttatctgtta ctttttagtt
    36421 ttcaaaatat agggttatac ttgagtcttc attaactttt actccaggtt ttgggaatat
    36481 atcctttacc tttgtatctt ctggaagttc tatatctgca tctaagtctg gttttaactt
    36541 cgcatcctcc aatgccttta cagcatctcc tatttttaga tttcttacat ctggcacttt
    36601 aacttgcttc ttttcatact cagctttttc ttcttcttta tatactggtt taactcccaa
    36661 atattttaaa gagtcattca ttatttcttt cactattggt cctgctgtag tactaccaaa
    36721 tgcacttact cctgttggtt cgtcaacaat agcaagaact actatttgcg ggtcatcaca
    36781 aggtgctatt ccaacaaatg aacatatata ctttcctgga gcatacttac catctataac
    36841 tttttgagct gtacctgttt taccaccaag tctataccct ggtatatatg ctatctttcc
    36901 tccaccttca gttacaactg attctgcaat ttctaacatt tttttagatg tttctttaga
    36961 tataacactt cttacttttt ttggttttac tgtttcagtt atatttcctt tattgtcagt
    37021 ataagatttt accactcttg gttgcattaa atctccacca ttagctattg atgatatagc
    37081 tgttataagt tgtattggag ttactgatat agattgacca aatgatattg tagccaattc
    37141 aactggtcct acattttttt cgttatacag tattccctta gcttctcctg gtaaatctat
    37201 acctgtctta tccataaggc caaatgattc tatataatca tacatttttc caacacctaa
    37261 tctgcttcct aattcaacaa atactgggtt gcaagagttt tgtactgctt gtttaaattc
    37321 ttgtgttcca tgaggtctgt aatgtctcca acatttaatc tttcttcctc caacagttac
    37381 actacctgta catgtgaatt tttctccatc tttaattact ccctcttcaa gagcactgga
    37441 agatgtaata agcttaaatg tagaacctgg ttcatatgta tcacttactg ctggatttct
    37501 ccacatttga taatagcctt ttattttatc tttatcatta tatttttcaa gttcttcttg
    37561 ataatatgga tatattggcg ttcttgagtc attagggtca taatctggtt ttgatgctaa
    37621 agcaagtata tcacctgttt tagggttcat agctattgca gtaacctttt ttgcattatt
    37681 taactcataa gctttctgta ctgctttttc agtatagtgt tgaattactt catctataga
    37741 caacacaaga ccattacctt gtacaggctc ataatatttt tccattcctt gtggtatttc
    37801 tcttcctgag gcatcagtac ttactataag ttttccaggc tttcctttta actttttatc
    37861 atattgcatt tctacaccag atatacctgt tgcatctgaa gaagtatgtc ctaacacata
    37921 tggcgcaaaa ttcccatatg ggtaatatct ttgattatct tctgctaccc atattccact
    37981 taatttagca tctcttattt gacttgcctt atcatcatct atccatcttt ttacttttac
    38041 aagagccata ttttttttgc ttattaaagc gtaaatatct ttataatctt catccaaaat
    38101 ctcagctacc ttttcagctg cttctttttt atcttctact tccacaggtt tacaccaaac
    38161 agtatactta gttacactta ctgctaattc tttcatattt ctatcatata tagttcctct
    38221 tttgggttct attggtatat ctcttgtttg ttgttctagt gcttttgtac tcaaccaatt
    38281 tcccttcata agttgtaggt acccagtcct aataacaagg caaaaaaaca atgcacatgc
    38341 tagaataaga acgagtacca gccttttctt acttatcctc tttacttttc tcaaatctat
    38401 tccccctagt tatccacatc tatatacttt atttgtccat cttttggata gtccatattg
    38461 agcaattcct tagcctttag ctcaatttct tttattgatg ttttttcagt ttcttttgcc
    38521 tttagttcct ctaatactat ctcttttttt cttaaatctt tttgtaaata atagatatcg
    38581 tacttcattt ccgagatttt tgaataacca catagattca atattattac tgaaaaaatt
    38641 gcaatcagta aaatgcattt agctcctatt tttttcttat ttctcttttt ataattattc
    38701 ttttttttat ttttatattc atttagatta tgtattttat aatttttttt caaattctca
    38761 ccccgatagt atatagttta aattttcctc tatactatat ttgttattaa tcatatttct
    38821 tcctctaaaa tttcatcgct aaattaatac actattacac aatctaattg tatttttttc
    38881 tatacacaac aaaaacctat aaattcactt atacttttat atgaatctat aggttagata
    38941 ttaatttcta tttattttta tcacttctag tatttttaga taatttcttc cataaatata
    39001 atatgtaact aactatctac tctctgattc ctaagttctc cattatatta cttaatacca
    39061 tctatatcta tatttttata tctaaatttt cattatatat ctcaataaca aagaacattt
    39121 cttatagtgt taattgtttt ttaattgctt taccagttca acaaaatata taatactttt
    39181 taattaatta tagtaatctt attttatata taagattatt ctttagcctt ataactactt
    39241 atattttttc tgctactctt agcttagcac ttcttgaacg tggatttact tctatttctt
    39301 cctcactagg taatataggt tttctagtta ttattttcac ctcagattct ttatcacatt
    39361 ggcatatagg taaatgttgt ggacatatac aatctgatgc taaatgctta aatgcatttt
    39421 tcactattct atcttctaat gaatggaatg ttattataca tattcttcca cctttattca
    39481 ttatagaaga agcatcattt atcatctttg taattactcc aagttcatta tttacttcta
    39541 ttcgtattgc ctgaaaagtt cttttagctg ggtgaggtcc atctattcta gctttcttag
    39601 ggatagcttt tttaattata tctactaatt ttcctgtagt ttctattggt ttgtttgctc
    39661 tttcttcaac tataaattta gctatacgct tagcccagtt atcttctcca taatctttta
    39721 ttatcttagt taattcatct tctgtataag tatttacaac atcatatgca gaaaactcac
    39781 acctaacatc cattctcata tcaagtggtg catcttgcat ataagaaaat cctctatcag
    39841 cttcatcaag ttgatgagat gaaactccta aatcagctaa tacaccatca attttgtaaa
    39901 ctcctatttt ttctaattcc tcttttatat tttcaaaatt gctgtgaaca aacttcactc
    39961 tactttcata ttcactcaat ctctcttttg ctgtgcttat agcattttta tcttggtcaa
    40021 accctataaa taatccctta tctgacagtt tttttactat ttctttagaa tgtcctgctc
    40081 ctcccatagt acagtcaaca tacacgccat ctggctttat atttaaattc tctatacact
    40141 catttaaaag aactgataca tgatgaaatt ccatagttac ctcccttaaa ttcacttttt
    40201 aaactattct tcactaatat tatgtttatt tttctttgat aaataaacat gtgcaattat
    40261 gccaccaata actccaatta gacttataac ttgtgccatt ctaagtggtc ctaacattaa
    40321 actatctgtt cttaatccct ctataaaaaa tcttcctatt gaatataaag ttatataagt
    40381 aactataact tgaccttcat attttttatt ttttctaaat agtaataaaa ctataaatat
    40441 tccaaaatcc catatggact catataaaaa agttggatgt acctttactc catcaaccat
    40501 tattccccaa ggtaaattgg tagcacctcc atgtgcttct ccattaataa aatttcccca
    40561 tcttccaata gcttgtgcta aaggcattcc aagtataact gtatcagcca tttttaagaa
    40621 gttaatattt ttaatcttag tgtatatata accagctaat attccaccaa ttaaccctcc
    40681 atgtatagca agtccaccac ctctaaaatt aaaaatttga gacatatttt gagcatagta
    40741 actccaatta aatacaacat aatagattct tgcacagatt agtccaactg gaattgctat
    40801 tatagctata tttaatacat catcatcttt aattcccact ctttttgctt cttttattgc
    40861 tattaaagtt cctaaaatca ttccacatgc cataagtatt ccataccaca tgatgtctat
    40921 gccaaacagt gtaaatgcta ctctatccat aacgtcacct ctttgtatta attgaaaata
    40981 tttccaaagc aatatgctat aattacaatt aattgtaatt atactaaatt atgataacac
    41041 attaatgtta attctacaaa aaatattaaa atttattcta caaatcccta cactatatta
    41101 tactaatata attactataa aattttttaa actgaatctc tcatgactaa agttacaagt
    41161 gtacatagat attttttaat caaaaagatt gatgcatata tacatcaacc tcttctattc
    41221 cttatctata gctcttaaat ttttacctca atttatataa at
//
LOCUS       DAGQWR010000014        40105 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.014, whole genome shotgun sequence.
ACCESSION   DAGQWR010000014 DAGQWR010000000
VERSION     DAGQWR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.014"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            10..747
                     /gene="cas6"
                     /locus_tag="MIW95_000669"
     CDS             10..747
                     /gene="cas6"
                     /locus_tag="MIW95_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBY3150018.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            752..2227
                     /locus_tag="MIW95_000670"
     CDS             752..2227
                     /locus_tag="MIW95_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150019.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKKMIDNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2247..3260
                     /locus_tag="MIW95_000671"
     CDS             2247..3260
                     /locus_tag="MIW95_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBY3150020.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIIFGKKGNIYPSIKQNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEKEENEVLR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDKLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            3276..4082
                     /gene="cas5"
                     /locus_tag="MIW95_000672"
     CDS             3276..4082
                     /gene="cas5"
                     /locus_tag="MIW95_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBY3150021.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVMPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCNVCIDTKEVFTWL"
     gene            4109..6517
                     /locus_tag="MIW95_000673"
     CDS             4109..6517
                     /locus_tag="MIW95_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBY3150022.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLLEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQEEIYDYIE
                     KGILEKEEFNLKDIESYINDILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDDMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEGALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     NYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            6958..7905
                     /gene="prmA"
                     /locus_tag="MIW95_000674"
     CDS             6958..7905
                     /gene="prmA"
                     /locus_tag="MIW95_000674"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBY3150023.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            7924..8682
                     /locus_tag="MIW95_000675"
     CDS             7924..8682
                     /locus_tag="MIW95_000675"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBY3150024.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8684..9982
                     /gene="mtaB"
                     /locus_tag="MIW95_000676"
     CDS             8684..9982
                     /gene="mtaB"
                     /locus_tag="MIW95_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBY3150025.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10025..10375
                     /locus_tag="MIW95_000677"
     CDS             10025..10375
                     /locus_tag="MIW95_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBY3150026.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10538..10717
                     /locus_tag="MIW95_000678"
     CDS             10538..10717
                     /locus_tag="MIW95_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBY3150027.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10747..11190
                     /locus_tag="MIW95_000679"
     CDS             10747..11190
                     /locus_tag="MIW95_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBY3150028.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11301..11798
                     /locus_tag="MIW95_000680"
     CDS             11301..11798
                     /locus_tag="MIW95_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBY3150029.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            11991..12554
                     /gene="raiA"
                     /locus_tag="MIW95_000681"
     CDS             11991..12554
                     /gene="raiA"
                     /locus_tag="MIW95_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBY3150030.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            12767..13003
                     /locus_tag="MIW95_000682"
     CDS             12767..13003
                     /locus_tag="MIW95_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBY3150031.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13016..14359
                     /gene="yqfD"
                     /locus_tag="MIW95_000683"
     CDS             13016..14359
                     /gene="yqfD"
                     /locus_tag="MIW95_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBY3150032.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14298..15413
                     /locus_tag="MIW95_000684"
     CDS             14298..15413
                     /locus_tag="MIW95_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBY3150033.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKVKMQEKTFKRK"
     gene            15470..15931
                     /gene="ybeY"
                     /locus_tag="MIW95_000685"
     CDS             15470..15931
                     /gene="ybeY"
                     /locus_tag="MIW95_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBY3150034.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            15937..16656
                     /locus_tag="MIW95_000686"
     CDS             15937..16656
                     /locus_tag="MIW95_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBY3150035.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            16775..17176
                     /locus_tag="MIW95_000687"
     CDS             16775..17176
                     /locus_tag="MIW95_000687"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBY3150036.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17240..18133
                     /gene="era"
                     /locus_tag="MIW95_000688"
     CDS             17240..18133
                     /gene="era"
                     /locus_tag="MIW95_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBY3150037.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18145..19524
                     /gene="mgtE"
                     /locus_tag="MIW95_000689"
     CDS             18145..19524
                     /gene="mgtE"
                     /locus_tag="MIW95_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBY3150038.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19597..19752
                     /locus_tag="MIW95_000690"
     CDS             19597..19752
                     /locus_tag="MIW95_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBY3150039.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            19718..20491
                     /gene="recO"
                     /locus_tag="MIW95_000691"
     CDS             19718..20491
                     /gene="recO"
                     /locus_tag="MIW95_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBY3150040.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20484..21083
                     /locus_tag="MIW95_000692"
     CDS             20484..21083
                     /locus_tag="MIW95_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBY3150041.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21243..22121
                     /gene="glyQ"
                     /locus_tag="MIW95_000693"
     CDS             21243..22121
                     /gene="glyQ"
                     /locus_tag="MIW95_000693"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBY3150042.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22121..24187
                     /locus_tag="MIW95_000694"
     CDS             22121..24187
                     /locus_tag="MIW95_000694"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBY3150043.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIRLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(24353..25900)
                     /locus_tag="MIW95_000695"
     CDS             complement(24353..25900)
                     /locus_tag="MIW95_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBY3150044.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            26180..26947
                     /locus_tag="MIW95_000696"
     CDS             26180..26947
                     /locus_tag="MIW95_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBY3150045.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            27230..27427
                     /locus_tag="MIW95_000697"
     CDS             27230..27427
                     /locus_tag="MIW95_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY3150046.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            27456..28532
                     /gene="vorB"
                     /locus_tag="MIW95_000698"
     CDS             27456..28532
                     /gene="vorB"
                     /locus_tag="MIW95_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBY3150047.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            28535..29254
                     /locus_tag="MIW95_000699"
     CDS             28535..29254
                     /locus_tag="MIW95_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBY3150048.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            29267..29806
                     /locus_tag="MIW95_000700"
     CDS             29267..29806
                     /locus_tag="MIW95_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBY3150049.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            29963..31021
                     /gene="buk"
                     /locus_tag="MIW95_000701"
     CDS             29963..31021
                     /gene="buk"
                     /locus_tag="MIW95_000701"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBY3150050.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            31039..31932
                     /locus_tag="MIW95_000702"
     CDS             31039..31932
                     /locus_tag="MIW95_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBY3150051.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            31962..33170
                     /locus_tag="MIW95_000703"
     CDS             31962..33170
                     /locus_tag="MIW95_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY3150052.1"
                     /translation="MKYAKRMDMVKASAIRASQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDIVVTENPSYLGAINAFRPYECDFLGVDTDDEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            33194..34456
                     /locus_tag="MIW95_000704"
     CDS             33194..34456
                     /locus_tag="MIW95_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3150053.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFSTPVLGMRWVMISLGILSIVMCPVLWFVIKDVDIREDKEEEASEKMSAGDFM
                     TVLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFG
                     APFGGYCADKVKSPTKVLLPIYVFGIAVIILFLVLPASTPMTIFIALTFVVGILGYMG
                     KGIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIF
                     QIAILIIGILSCLYILKVKKKRESKLEA"
     gene            35032..36495
                     /locus_tag="MIW95_000705"
     CDS             35032..36495
                     /locus_tag="MIW95_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBY3150054.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSMKLRFFQIKDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSILFSVVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVTSSIALGIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIIANINLVPGAIMTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIIITGMYNVTDVYTTLPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKHIIN
                     VCRFMLIICAAMCCFIEPLTSWALGDICIGVLTYINLVGIFTLKKPAIKAYYDYQRQK
                     KMGVKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            36551..37687
                     /locus_tag="MIW95_000706"
     CDS             36551..37687
                     /locus_tag="MIW95_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY3150055.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            37923..39038
                     /locus_tag="MIW95_000707"
     CDS             37923..39038
                     /locus_tag="MIW95_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBY3150056.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALTPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKAKKIGEKELMNLLSLKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            39190..40059
                     /locus_tag="MIW95_000708"
     CDS             39190..40059
                     /locus_tag="MIW95_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY3150057.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT        16042 a         4121 c         7463 g        12479 t
ORIGIN      
        1 ggtgaaaata tgagcagaat ttcaattttt atggagtcat gtaatggcaa atttccaaat
       61 gaaaattcaa tgttgtcagt ttcctttata aaaaatattt taaatgcaga aaacaaagat
      121 tttgctaaaa atatatttaa ctatggggaa gaaagaaaaa acaataagca aataaaggat
      181 ataaatacag ccatctacat accaaatctt ataagaagca aaaatgagtt gcttgttgat
      241 ggagatataa tattcaatat aagtttttat aattattcta tgttcagtaa attatacaat
      301 ggaattttaa aagtaaagga gctagaatat aaaggttata gatttaaaat aaaaaatata
      361 aaaatacata aagaacacga gataaaagaa aatggtgtta tatttaaaac tatgtctcct
      421 ataatagtaa agaataaaga aggtaaatat ttagatgtag aagattcaaa ttatatagaa
      481 tgtttaaact atatagctaa tctaactctg gagagtataa gaggtagtgg gcttagaaag
      541 ccattggaat ttatacctct taacttcaaa aaaagagtgt taaaagaaaa gattcgtgga
      601 tttaaagtga gagaatatta ctatataaat gcttatgcag gaacattttt tttaaaagga
      661 aatatggaag atttaaatgc actgtataaa atgggaattg gatatagaag gacggaaaat
      721 gcaggaatgg ttgatatatt aaagtagcga ggtgagagaa ataaaagcta aattttatag
      781 atcaggtgat attgtaagag atattggcat tgtttacttt tatgaattac taatggattt
      841 aaaaaacgaa ctagaaaaaa atgattataa gctaaaattt acttgtgagt taaatagaaa
      901 ttatttatct ttagaatctg cagaatatat agaccctaag tatatatctg attatatatt
      961 agaaaatcaa ctttttaagg tatttaaaaa tgggaaaaag gaaaccttag aaaaaatatt
     1021 tagtaatata gataatttaa cgtatgtaaa ttttattgaa gaattagata aatcttctgc
     1081 aactgagaaa caaaaggaag ggattaaaaa agattttaaa aacagaagat ttccatatgt
     1141 tagaaatagt ggtaagtatg gattaaacac gagtttagaa aatatggaaa caaatgtaaa
     1201 aagaatagta gatactgtaa ttaaatcaaa tatagagata gatgaaatag ataaacttga
     1261 tttgacacaa tatgaagaaa aagatagtgt atgtaatgtt tgtaatataa ataaaacaac
     1321 aaaactagat attgatttaa gagagcgaaa ggactctaag tataactttt tatttagagg
     1381 tgcagaaaaa tcaggcttta aaagaagtgg acaagttgaa agtaatattt gttttgagtg
     1441 tgaatttttc aacttaatgt gtcttttata tataaattta aaaagaccaa tggtttttgc
     1501 atatacgaat gatttaaggg aattagcatt tttaaatcat aagataatgc ttaaaagaaa
     1561 gatgtattca gataaatcat tttataaaaa acttttgcat gaaaaaataa gttcgattag
     1621 actatatagg tttgatattg atacgaataa aggtataatt ctaaagtttg actctattat
     1681 agagtataaa gaattattaa aaaaaattga acttatagat attatagata attataattt
     1741 ttcaagagag ccagggaata ctagaaattt gggtaaaaaa atgattgata atgggaattt
     1801 atcaaattta aaagaattat tacttgataa tataagtgtt ttaaggcaac taactggaac
     1861 ttctcgagaa atggattttg tatctagctc atataatatc ggtttatata taaaattgtg
     1921 ttggattgta gatggagggg aggagtataa aatgaaaaac tatatggaaa gtaatttaat
     1981 atattcaaga gtaggtaaag acttatttaa taaaatgaca gatgaatcca gaagaaaaaa
     2041 tttttctatg aaagtgattc aactacttaa gtctaatgat agaactcagc tatttcaaac
     2101 tataatgcat gtgttagttt ctaatggaat cttaatagga gaagggtttg tagaaggtct
     2161 aatgcagagt tctgaattag aattaaatac gaatgtaggt ctatttattc aagaaatcat
     2221 gaaataaaaa agggggaaaa tttaaaatga atgaaaaaag agtaaaagga gtaacattaa
     2281 ctatactgac agaaagccca gttccacttt catatgacca agggtatgga aattatacac
     2341 caataaaaaa agaacaatat agagaaaaaa tacatgcaaa aactagtata gccacaataa
     2401 catatgattt aagaagaatg ctccaccaag aatatggttg gaatttaagt aatattattt
     2461 ttgggaaaaa aggaaatata tatccatcta ttaagcaaaa tgtaggatgt gtagatgaaa
     2521 atggattaga gactgatgta tttggttatt tgataccact taaagataaa ggttctataa
     2581 gtaaggcttc tccgcttaga ataatacctt ttaggtcatt aaattcatat agaggttcaa
     2641 cacagttgat aacaaataga ggttttttaa gtagcgaatt tggtagaaaa tattatgatg
     2701 agaaggaaga aaatgaagta cttagagatg aaaactttcc aacatcacaa gcattagcaa
     2761 tagaagagac tttatcagat tactacgttt atacaataac attggagctt gatagaatag
     2821 gagtagtaga agtagaagat ggaaagttgc ttttaccaga agaaagaaaa tttatgagta
     2881 aggagttaag agaaaaagca gtaaaagata tattagatgc aattactgtg tttacaagaa
     2941 acataaaaca ttcatcagta ctgttaaaac cacttgcagt aatgggtgga gcttttgata
     3001 aagttgtacc gtttttttgg gatgatgtag attataatgc tgatagtggt gagataaatt
     3061 tagagggagt catagaaact atagaaagtt atagccttaa agaaagtaac acaatacttg
     3121 ctatcaatga caaattaaaa atttctaata aaaaggaatt aaataagttt aatttaagta
     3181 aataccctgt aaaagagatt aaaaatctag cagatagatt ggaaattgga gaagataata
     3241 tgtggtattt aaaagagtag gaggtttttt gaattatgaa agtattacaa tgtaaactta
     3301 agcaaccaac tgctcactat agagacccaa aagtatttca aaatgaatat ataagtactt
     3361 taaatttacc atctaagacc acaataatgg gaatgataac gtatttatgt gatagaagat
     3421 taaactctga tatagatata ggaataattg gaacacatca tcatagagaa ctagaatttt
     3481 caagaggaga gaacattgat ttttggaatg aatatacaaa tatgagaaaa ggcaaagata
     3541 aagaaaagtt tttgcttcaa ggaaactact atgattacta taaagaacat aaagctcaaa
     3601 atagcatatt aaactatgaa gttcttaaag aagtagaact gactatattt atttcttgca
     3661 aggatgatga agaacttgaa tttattaaaa aaaagcttga aagtccatgt aaatatgcaa
     3721 atttaggaag aaaagaagat tttgtaatac caagtgaaaa agggtgtttt gtgaaagaag
     3781 ttgagttaaa agaggttatg cctatgaata ctcgtgatgc tataaaagaa aatataaaat
     3841 taaagaatac atatgtaaga gttgacttaa gagataaaga gaatgtcgag actataataa
     3901 atcaaggtgt attgattgca ttaccttata aatataaaga tttagaagca aatagagatg
     3961 atagagttta tgagttttgc cattatatat atgttgataa tgatggtata tatcctaaaa
     4021 atattaaggt aaatgtatgt aatgtatgta ttgatacaaa agaagttttt acatggcttt
     4081 aaattatttt gaaagatagg tgtttatcat gaaaaatatc atgcaaaaag aaatattagc
     4141 taagtcatat aatttaagaa ctaatcaaca agaacaaaca ttagttgaac atattaagga
     4201 tttatttgaa gtattagaat ctatattaga actaaatcta tattcagata aagatgtaga
     4261 gatactaaaa atatgttgtg ctttgcacga tttaggaaaa attaattcaa ttatgcaaca
     4321 aaaaatggag attaataata aaatatcgta tagttgctca gaagaagaga ggaaaaagtt
     4381 agagtctgac aaaaaatctt taaaaaagat agcaagacac aatatatttt ctggagcatt
     4441 tttaaaagat atattagaaa aaatgaactt gttagaagaa gataagttat atatatataa
     4501 aagtataatg ttacatcatg gaaactatga agattatatg aggttaagta ctagtaaagt
     4561 tcaagaagaa atttatgatt atattgaaaa aggcatttta gaaaaagaag agtttaatct
     4621 gaaagatata gaaagttata taaatgacat tttaaatgta gattttaaat ttggtgaaga
     4681 tgtattggat tatgattaca tagataaatt aagtgaaagt atgtttatag acagtgatta
     4741 taacaatggt caaatagatg attcagtttt aaattataga aagtttaagt atattttata
     4801 taaagggatg ctgaacttaa tagaccatag tgcatcaagt agacagaaag gtataaaatt
     4861 ttataacgat tttactgatg aagaaattga tgatatgatt ttaaatgaga tatataagag
     4921 tcagggtaat ttagaaaaaa ataatataga gttcaatact atacaaaaaa ggttgagaga
     4981 actttctgga agaaatgttc tgactgttgc atttacaggt tcaggaaaaa cagctgcaga
     5041 ttatagaaag acttttaaaa gaaaattctt tttagtgcca aataaaatat cagcagaaag
     5101 tttttataga aaaaatattt ttcaaaataa aaatgattta gatacaagaa gtagtaatga
     5161 ttacattgga ttacttcatg gagatataaa cttatattca gagaatgaag ataatggaga
     5221 acatgatttt gtattgacat taagggatat agatttgagt ataaattttt gtaaaccttg
     5281 tgtaattgca acagttgacc agttattact tagtatgttt aaatttccgg gatatgaaaa
     5341 aatattcgca gccgtaaaaa atgcatctat aactgttgat gaagtacatt tattggaacc
     5401 aaagatgttt ttgattttga tatattttat gcagtttgca tgcaagtact tagatgttga
     5461 ttttcactta atgacagcta cacttccaaa atcatataaa gagcaaatga taaacaaggg
     5521 aattattttt caagaagaaa gtaatgaaaa tgttactgat aaaggagaaa tagtttttat
     5581 tgagagcaac aaggaagaag aaatttgtga aggtaaagat gtaaaggttt cttttataaa
     5641 ggaaaaagaa atcaagtcaa tagttgaagg tgctttagaa aataaacaaa aaattcttat
     5701 aataaagaat actattgatt cagtcaatag aacatatgag tttttaaaag aaaatttatc
     5761 tgataagtat ggtggtgtag atatagatgt tttacatagt agatttaagt tcaaagataa
     5821 aaaagagaaa tatagtaaaa tattaaatgg agagggtgat atttggatat caactcaatc
     5881 agtagaaata tccttggatt tggatttcaa tataataata agtgatttag caactatgga
     5941 tagcttaata caaagaatgg gtagatgcaa tagaaataat aaatatgaat atggaaactt
     6001 ttatattcta cctagtgaag ataaaattta tgataacaaa cttaagaaca caactaaaaa
     6061 catcttaaag gacatattaa aaactaactc tatttttaca atggggatta gaaaaactat
     6121 tttggatgat tactatgata atagtgtagt taaaaactat tttgaagaga attttataag
     6181 ttgtgataaa gaaattaaga atatttatgg tataaataaa gaaatttttg atggattgga
     6241 tttaattttt aattttgaac cttacaaaaa tattgttgat agtaagaagg aagcagctaa
     6301 aatttttaga gatgttgatg ttagttacaa aattattctg gaagaagatt tttataagga
     6361 agatagggag ttacaacaag attcgattca agtatcaggg tttatattta atagattata
     6421 ttattatgga ttaataagca aagtagaagg atacatggta ctaaaatcta gtagtaagtt
     6481 tgagtataat agtactgtag gcttaatttt gaagtgagca tatgtttaaa tataatatac
     6541 ctttagacat tgaaaataca aaaagtacta ctgtaagccc actgtaaaaa ctatttttta
     6601 aaaggaaaaa taagttgtga gatagagtgt ctacaagtgt atatagataa taaatttgag
     6661 gtttaagatt aactaagtgg gtttcaggta catcgcgaaa ataattataa gttggagatt
     6721 ttgtatgttt aagattaact aagtggattt caaatattga tatagtagat ataatagaag
     6781 ttttattatt aggtttaaaa ttaactaatc ggatttttaa atattctgac tattatataa
     6841 ggatattatt agcttgaatt taaaaccaat aatataaaac aacaaaatat tatatttatg
     6901 atataataaa agttatggaa aaacaggaat attactatag agaagagagg taaacttatg
     6961 aataattgga tagaagtaac tataaaaaca acaacagaag cagttgaacc tataacgaat
     7021 atactatatg aacaaggagc tggaggagct gtaatagaag acccaaaaga ctttttattc
     7081 caaaagaaaa atgaactaga ctgggattat gttgaagaag aagtatttaa aaagaatgaa
     7141 gaagatgatg ttcttataaa aacttatgtt tctgaagaaa aaaatgtgat ggaatttgtc
     7201 gaaataataa agcaaaaagt tttaggatta aaagattttg gtatagatat aggtgaagga
     7261 agtgtatcat tggaccaagt aaatgaagct gattgggcaa atgcatggaa agcatactac
     7321 aaaccaacaa aagtaggaca aagggtagta gtaaagccta catgggaaga ttatgctatg
     7381 caagatgggg atttaatcat agagcttgac cctggaatgg catttggtac agggacacat
     7441 gaaactacta gtatgtgcat tagagaatta gaaaagtatg tgaataaaga ttctaaagta
     7501 tttgatattg gatgtggtag tggaatcctt gcaatagcag cagctaaatt aggagcaaaa
     7561 gaagttgtag ctgtagattt agatgaagtg gctgtaaaag ttgcaaaaga aaatgttctt
     7621 gaaaataaag ttgaaaaaag tgtatctgta atgcatggta atttaactga tgtaataaaa
     7681 gataaagcgg atgtaatagt tgcaaatata atagctgata taataaagat acttgcaaaa
     7741 gatgttcaaa actttatgaa agaagatgct atatttatat cttcaggtat aattttggat
     7801 aaagtagaag aagttaaaga aagcttaatt gaaaatggat ttgaaatagt tgaagttcaa
     7861 aaattaggtg aatggtcagc aatagtttct aagcttaaaa aataagggat atggtgacgt
     7921 ataatggata gattttttgt tgaaaaaaat aatataaatc tacaagataa aacttgcact
     7981 atagaaggtg aagatgtaaa acatatttca aaagttttaa gatgcaaact aggtgagaaa
     8041 cttgaaatat gtgacaagaa taataatgag tatatttgtg agataatgaa tatagataag
     8101 agtatagtta atttagagat tttagaaaaa gttgatataa atagagaatc agaacttaaa
     8161 gtaaggttat accagggact tccaaaagct ccaaaaatgg aaatgatact tcaaaaatta
     8221 actgaagtgg gtgtagaaga aataatatta gttcaaacta agagaagtgt tgttaaagtt
     8281 gatgacaaaa aagaagataa aaaatttgaa cgttgggaaa gaataattta tgaagcagca
     8341 aagcaaagca aaagaggtaa gataccaaaa ttaagaggtg ttttaagttt taaagaagct
     8401 ttagaagata tgaaaaaaaa taatgtaaat atttgccctt atgaaaatga aagaacagtt
     8461 tcaataaaac atgcactaaa aaaatgtgat tctaatatag atagtgttgg aatttttata
     8521 ggtccagaag gtggattttc tgaagaagaa atagagcaaa tacaaaaaaa taattgtaat
     8581 gttgtatcat tggggcctag aatattaaga actgagacag cttctgttgt agcatcaaca
     8641 atagctttgt atgaactaag tgatttagga ggagaaaaat aaattgaaaa aagtagcttt
     8701 ttatacactt gggtgtaaag ttaatcaata tgaaacagaa gctatgctag aactatttga
     8761 gaaagatggt tatgaacaag tcaatagtga agagtatgct gatgtttatg ttataaatac
     8821 atgtacagtt actcatatga gtgatagaaa atctcgtcaa tatataagac gagttaaaaa
     8881 gaagaatcca gatgcaataa tagccgttgt aggatgttat tctcaggtat ctccagaaga
     8941 aattttggat atagaagaag taaatttagt catgggtaca aatgatagaa gaaagattgt
     9001 tgaagaaatt aaaaagataa attcaagtaa gaaagttagt acagttgatg acataatgaa
     9061 agttaaagct tttgaagaaa tagaaatcag ccaaactaat ggaaagacta gagcatttat
     9121 gaaaatacaa gatggatgtg acagattttg tacttattgt atcattcctt atgcccgagg
     9181 acgcgttaga agtagagata ttgatagtat agtcgatgaa gtgaaaaaat tggctaataa
     9241 tggctataaa gaggttgtat taacaggaat acatgttgcc tcttatggaa aagatttaaa
     9301 ggatagggat attaaacttc ttgatgtaat aaaacaaata aaccagatag aaaaaataga
     9361 aagaattagg cttagttctg ttgaaccaat tttatttaca gatgaatttg taaatgaagt
     9421 tttaaagatg gataaagttt gtcctcatta ccatttatca cttcaaagtg gatgtgatga
     9481 aactctaaaa agaatgaata gacgatacac aactttagag tacaaaacta ttgtagatag
     9541 attgagaagt aaaatgccag atgtggcaat aactacagat gtaatagtag gttttcctgg
     9601 agagacaaat gaagagttta aaaaaacgta cgagttttta aaagaaatag agctttcaca
     9661 aatgcatatt tttaaatatt caccaagaaa aggaactcca gctgcaacta tggaaaatca
     9721 agttgaccct caaatgaaac actttagaag tgaacaactt ttaaatttaa gtaaagtaaa
     9781 ttttaataaa tttgcaacta aatttatcgg ccgtgagctg gatgttttgt ttgaacaaaa
     9841 tatagaaggc aataaatatg agggattaac ttcaaattat ataagagttg ttgttgaaag
     9901 tgacaaaaat atacaaggtc agatattaaa agtgaaaata aatgatgtaa aagatgaata
     9961 tgtagaagga attttgctat aacatgtaga aattataata tttataagat aggaggtgga
    10021 aagaatggat tgtatttttt gtaaaatagc gaacggagaa ataccttcaa ctaaagttta
    10081 tgaagatgat agagtcttag cttttaatga cttgaaccca gttgctccat atcatatatt
    10141 agttgtacct aaaaaacatt atgatagttt aattgatatt cctgataaag agatggacat
    10201 tgtgtcacat attcatgttg ttattaataa aatagctaaa gagaaaggtt ttgaccaaac
    10261 tggattcaga gtcataaata attgtggaag tgatggaggg caagaagtta aacatttgca
    10321 ctatcatatt ttagctggta aaaagcttcc taattacgaa gcgggacaaa actaaactaa
    10381 attattgaaa aattgagttc cttcttgata aaaatacttg ataaaagtaa aaatttagtt
    10441 tataataaac aaagtgttag gttgtaatgc atattatgca aaaagaacat agttacagtc
    10501 ccagcataat tgcgctattg gagggaggga aaaagaaatg tcagaagtaa gagttagaga
    10561 gaatgaaaca ttagacagcg ctttaagaag attcaagcgt caatgtgcaa tgtctggcat
    10621 catgtcagaa gtgagaaaaa gagagcacta tgataagcca agcgttaagc gtaagaaaaa
    10681 agctgaagca gcaagaagaa aaaacgctaa gaaatagtag taaataagaa taaagaggtg
    10741 aaggggatgt cccttaagca aaagttacaa gaggatttga aatcttcgat gaaaaacaaa
    10801 gatacagtga gaaaatctgt agtaacttta ataagagctt ctataaagca gtatgaggtt
    10861 gataataggg tcgagcttga tgaagatgga attatcgatg taatagcaaa acaattgaag
    10921 caacgtagag atgcattagt tgaatttgaa aaagctggaa gagaagactt aataaaagaa
    10981 acagaaggtg aaattgaagt tttaaaagag tacctacctc aacaattaag cgaagaagag
    11041 ttagaagaaa ttgtaaaatc tactatatcc gaagtagggg caacttctat gaaggatatg
    11101 ggaaaaataa tgtcagttat ccaaccaaaa gttaaaggaa gagctgacgg aaaacttata
    11161 aataagttgg taaaacaaaa cttacaatag attgatatag cctagagaga aatctctagg
    11221 ctttttatag tttttgggaa taacgagtca tatttgagca tatctataat caaagaaaat
    11281 tttgaaagaa ggggaaaaat ttgattatat cagtaaaaga gattaggaat tttttgataa
    11341 acatatttat accactaata ataggttact taagtagcat cttaacaatg attatcagtg
    11401 gaacagatat ttcaacctat tatttacaac tcacaaagcc aagctttgca ccaccagctt
    11461 ttatctttcc tatagtttgg actatattgt acatattgat gggtatatct tcatacctaa
    11521 tattaagaaa gggttacaac ttacctaagg taagagatgc aattttttat tatggattac
    11581 aacttgtatt aaactttatt tggagtatac tattttttgg atttggactt agatttacag
    11641 ctttgataga tataataata atgattttgg tttctataat aatgatttcg aagttttcta
    11701 aaatagataa gagatctgga tacataaatt tgatttattt aatgtggctt gtttatgcag
    11761 gatttttgaa ctattttata tggtttatta ataaatagaa tgtaaagatt ttgtaaatta
    11821 ccctgtactt ttaataatat cataacaaac gaaaaataaa tagactatta aaaataatca
    11881 ttttcatata caataatatt aatttaaata caataaattt ataaaaattg tatttaaaga
    11941 ttaacatatt aaagtattag aattggaaag gcttgaaggg agaggttttt atgcaaataa
    12001 tagtatctgg gagacaaatg aaattaactg acggaataaa aggatacgta gatggaaaat
    12061 taagtaggtt agaaaaatac cttgatcctg aatcagaagt gaaagtaaca gtaagtgcga
    12121 aaaaggatag acaaaaagtg gaggttacaa taattcctat taacggtcaa ataataagag
    12181 cagaagatgt agaagatgac ttatatgcag caattgatat tgtttgtgac aaattaagta
    12241 gacaagttgt caaatataaa actaaagtta aagacaaagt tcaaaataat aagagcataa
    12301 gatttgaaaa tcttgacttt atagataata gcagtgaatt tgatgattat gattatgatg
    12361 atgaagaaga tgaaaatata gtaatagaaa gaagaaagaa atttaatgta aaacctatga
    12421 gttctgagga ggcgatactt caaatggaac tagtaggaca caatttctat atgtttagaa
    12481 accaagataa ttttgaaata aacatagtgt ataagcgtaa agctggtgga tatggtttaa
    12541 tagaacaaga ttaataaaaa ttgtatacgt ttagatttta tataagaaaa aataaaaagg
    12601 taatcatgtt gattttttat caatatgatt acctttttat tgtttaaata atatattaga
    12661 tattaggtat ttattatata aaaattttaa aactaatctg ttacaacatc aataaagtgc
    12721 ataaatacct atttcaagca taaaattaat taagggggta atgcttatgt tagaaatatc
    12781 cactgattta caagtaaatc aaccagttat tactgttaca agtaatacgt ttattagtat
    12841 agaaaattat ctatcaatat tagaatatga agttgattta ataagaataa agactaaagt
    12901 taaaacaatt aaaatatctg gggataaact ttcacttaag tatattactg atagtgagat
    12961 aggtataaaa gggattatct acaatgttga gtatgttgat taggaggaga tacacttgat
    13021 aagttttata aggggatact atgttatagt tgtggaagga gtaggactcg aacaattttt
    13081 aaatcatctt ataagaaatg ggataagtgt ttacaatgta actagaataa aaaatactaa
    13141 aatggagttt catatagata gacaagatat aaaagaattt aagaatgttt atagaggaag
    13201 taagtttgat ataaaggtaa agcaaaaaac tggggtccct tttattatca aaagagttta
    13261 taagcacaaa ggtatgtgga tttgtgctct tgtttcttta tttttattga tgtctacatc
    13321 gcagtttgtg acagatgtat atatacaatc tcctgaaggt ataaaaaaag aagctcttag
    13381 aaatgagctt tataaggttg gagtaagacc aggagtctat aagaagagta ttgatagaaa
    13441 agaagttaga gaccatatga tgtcaaaatt taatgatgtg gcatatttat ctatcaatgt
    13501 aaaaggaaca aatatatttg ttactgttac aaaaaaggca gaatccctaa aatctacaga
    13561 ccaatcaaat tattgtaatg taattgcttt aaaaaatggt ataatagaaa aggtaatccc
    13621 tagaagtggt aaatcagtta ttaaatctgg agacatagtc caaaagggag atgtcttatt
    13681 aaatggagct aataccaagt caataccaga agtatgggca agtacttttt atgagtccac
    13741 aaaaaaggca agttatgtag atactgtaaa taaaaaaact ggagaaaaga aaaatatata
    13801 tacactaagt ttttatgata aagagtttac aatgagaaaa aatataaaat ataaagatta
    13861 tgttgttgaa aataaagaaa aaaaactatc aataggaaac tatacttttc caataaagat
    13921 aaaaacgagt actttttatg aaacaaaaaa ggttgaagta aaaaggaata aagaagagtt
    13981 aaaaaaagaa ttatctgaaa aagctttaaa agagttagaa tacataatcc cagcttcggc
    14041 tagaataata gatgtaaaac ataactttaa ggttaataaa aatatgttag aatatttaat
    14101 aacagttcaa actagtgaaa atattgctaa aatttatcct cttagtaagt cagaagcaga
    14161 aaggtttata aaggaagaaa gtaaaccaga tgaaggtgaa gaggaagttc cttccaatcc
    14221 agaaaaaaga ccattaaatg atataagaaa tgagtttgat gaggataata aggataataa
    14281 agaccaaaac aattctgatg aaaataatag taatcaaaat agtaataata gtcaaaataa
    14341 taatagcaat aacaactaat aaaattaaac ttaaaatctt aggaggacta attcaattga
    14401 taatacaaaa gaaatttaca atagcagatg gtaactttga aagagagtta tttggcaact
    14461 ttgatgaaaa tgtaaaatta atagagaaga ctttaaatat agatgtaata cttagagaag
    14521 gcaatataat tttaattgga gaagaaaaaa atgtagattc tgcattaaaa cttatgaatg
    14581 aattacacca aactgtatca aatggtaagc atttagataa gcagagtatt tcttattcat
    14641 tatctctttt gcttgaagga agtgaacaaa aaataaaaga acttgagggg acaatcgtca
    14701 taactcaaag gggaaaagct gttcaaccta aaactttagg tcaaaaagaa tatataaaac
    14761 ttatagaaaa taatgatata acttttggtg ttggacctgc tggaacagga aagacttatc
    14821 ttgcggttgc tatggcagta aaagctttta aaagagatga agttagtaga attatactta
    14881 caagaccagc tgtggaagca ggagagagtc taggtttttt accaggagat ttaaaggaca
    14941 aagttgaccc atatttaaga ccactatatg atgccttatt tgaaatgttg ggtgctgata
    15001 aatttaataa gtatttagaa agaggtacta ttgaggttgc accacttgca tttatgagag
    15061 gtagaacact tgataattca tttataatat tggatgaagc tcaaaatact acttctgaac
    15121 aaatgaaaat gtttttaact agacttggat ttggctctaa agctgtagtt acaggagatg
    15181 taactcaaac agatttacca caaaataaga agagtggtct aatacaagct acagagatcc
    15241 ttaaaggtgt tccaggtatt gggtctataa tgctaacaga tagagatgtt gtaagacatg
    15301 agttggtaca gagaataata agagcttatg agaagcacga caaaagagaa gaatttaaaa
    15361 aagaagaacg taaaaaagtg aaaatgcagg aaaagacttt caaaagaaag taaaagtgta
    15421 gtaatataga tatatagcat tattaatgaa taaaaagtag gtgtagtgaa tggatttgat
    15481 actagatgat agacaagata aattagaagt aagtgaggag ttaattgaaa agataaagga
    15541 tataatcata gaatgtttag attatgaagg atatgatgat aattatgaag tgagtctttc
    15601 ttttgtagat aataaagaga ttcatgaatt aaatagagaa tatagaggag ttgatagagt
    15661 gactgatgtt ttatcatttc ctcttttaag tgatgatttt gaggatgttg agttagaaga
    15721 agaatcccta ggtgacatag tagtatctct tgaaagagcc ttagaacaga gtatagagta
    15781 taaccatagt tttgaaagag aagtatgttt tttaatatgt catagtatgt ttcatctttt
    15841 aggttatgac cacgatacag atgaaaatac aaaagaaatg agagaaaaag aagaacacat
    15901 attaaataag ttaaacataa caagggagta gtttttatga aaccagaaaa aactcgtcaa
    15961 ggtataatta aagcatttaa tgcagctata gaagggatat tgtatacatt taaatttgaa
    16021 agaaatatga aaatacatta tttaggttca gttgctgttc ttataataag tttgtttttt
    16081 aacttttcaa aattagaaat gataatgtta cttatgtcca tatgcttagt tgtagttgcc
    16141 gaaatgttta atacagctat agaaaaagct gttgatttag taactgatga atatcatgtt
    16201 cttgctaaga tagcaaaaga tgttgcagct ggaggcgttc tagtggcagc actaaattca
    16261 gtagtagttg gatatatttt gttttatgat aaattgacag atatatctgg aatattaatt
    16321 tataaaataa gagaatcaga attacatata accttgatat gtatattatt ggttcttata
    16381 gctgttgtag ttgtaaaagc attgacttct acaggtactc ctctaaaagg aggaatgcca
    16441 agtggtcatg ctgccttagc atttgctata gcaactgcaa ttacacttat gactgaaaga
    16501 gttgttgcct ctactcttgc atatataatg gctgtattag ttgcccaaag tagaattgaa
    16561 ggaaaaatac ataccttttg ggagactatt gctggagctt tattaggtgt gttgatagct
    16621 atattagtat ttcagcttgg aatgttctat aattaaaata aatgtacata aaatatttaa
    16681 aataaaacaa atctgtgcta attaaatttt tatgatataa tgtagagtat taaataaaaa
    16741 taacttttaa ttattgtata caggaggagt tgttgtggat aacatagaat tgttaaggtt
    16801 ggcagaagat gccagacaac attcttatgc tccttactca ggttttagag taggtgcagc
    16861 tcttttaact aagagtggta aagtttatac tggatgtaat atagaatgtg catcattagg
    16921 tggaacaaat tgtgcagaaa gaacagctat ttttaaagca atatcagagg gtgataagga
    16981 tatctataaa atagctatag caagtgataa ctcagaaaat aatgagcaaa catatccatg
    17041 tggaatatgc aggcaggtaa ttatagagtt tggttctgat ataaaaataa taacgggata
    17101 tactaaagga gaggtgtttg agcatagtat aaaggatttg ttgccaaact atttctctgg
    17161 aaatgattta aaataaattt aaaagtataa ataaatttaa gtaattaaaa caagaagata
    17221 aaaagttagg agacagttaa tgttcaaatc aggttttgtc agtatagtgg gtagaccaaa
    17281 tgtaggaaaa tctactttaa tgaataatgt agttggagaa aaaatagcta taatgagtga
    17341 taagcctcaa actacaagaa atacaataca agcagtatat acagatgaag aaatgcagat
    17401 agtattctta gatacacccg gtattcacaa accaaaaaat aaattaggtg aatttatggt
    17461 aaaggcagca acagaagctt ttaaaaatgt agatttaata ttatttgtag ttgatgattc
    17521 taaaaaaata ggtcctggag atagaaagat aatagaggat ttaagaagtg ttaagacacc
    17581 aataatttta gttgtaaaca aaatagacca actagaccaa aaagatgagc tatttgatat
    17641 aatcaaaatg tatgatagag aaggtatttt taaagaaata gtaccaatat cagcattaaa
    17701 aggaaagaat acggatacct taataaaagt tatacaaaat tatttagaag aagggccaaa
    17761 gtattttcca gattatatga ttacagacca gccagaaaga gtattaatag cagaacttat
    17821 aagagaaaaa gttcttcact atcttaatga tgaaatacct catggtgtag ctgttgaaat
    17881 tgaaaaaatg aaagcgagaa atgataagga aatagtagat gtatctgctg taatatactg
    17941 tgaaagagat tcacataagg gaataataat aggaaaaaat ggtaggaaac taaaaggaat
    18001 aggtaagtca gcaagacaag atatagaatt gcttttaggt tcacaaataa atcttcaatt
    18061 atgggttaaa gtcaaagaaa attggagaaa tttacaaaat tatataaata attttggata
    18121 taatgataaa taaaggtggg ttatatggat aggaaactgg atgcttccca agatttatta
    18181 tatgaagtaa aatcattaat tgataataat aaagtattgg agttaagaga attaatagag
    18241 gaatatcata taattgatat attcgatata atggaaaatt tagaggaaga catgaaaatt
    18301 caattatttg aagtacttcc tttagatatg gcatcttcta tattagaaga aggtagtgta
    18361 gagtttttta ttagtatttt atctaaactg gatgtagaac actcgaaaaa tatacttgaa
    18421 cttatgtctc ttggagatat ggcagacaag ttgagtgaac ttgaagaaga agaaagagaa
    18481 cacataataa atcttttgaa tcaagagaat gccgattatg taaaagagtt actattctat
    18541 gatgaagatt ctgctggtgg aacaatgacc actggatata tttctataaa taaagatatg
    18601 acagctcttg aggctataga ccatatgaga gaagaagctg aagaagctga aactatttat
    18661 tatatatatg tggttgatga tgaagaaaaa ctagttggag tattgtcatt aagagaatta
    18721 ataatagcta gagacgcaaa tatagtagaa gatttgatga gtgaaaatat aatctctgtt
    18781 tatgtagatg aagatagaga agaagcagta agattagtat ctaaatacaa tttaatagca
    18841 attccagtag tagacagaca agaaaaatta aaaggaataa taacagtaga tgatataatt
    18901 gatgtaatgg aagaagaagc aactgaagat atgtataaat ttgcaggaag ttcagaacat
    18961 gaaagagaag ttgctgaaaa agaaaaccct acattgagag agcaaataat atcagcactt
    19021 agaggacgat taccttggtt aattattacc ttggttggag ggttgttggc ttcgttaata
    19081 ctttctaact tagactatat aatgaatcct gtatatgctc cattagtatt ttttatacct
    19141 gtagtaattg gtatgggtgg aaatattgga actcaatcat catctgttac agtaataaca
    19201 ttatctaata aggatttaaa ttttagcaat gtggttagag aaggtattgt aggtattatt
    19261 acaggtcttt tgtgtagtat aataacaggt attgtaattt actttgttat gagaaagtta
    19321 gacattgtat taattgtttc aatatcatta tttataaata tggttttagg tgcaacaata
    19381 ggtgcgttta tgcctgtttt attaaaaaaa atggatgcag acccatctac agtttcatca
    19441 ccaattatat ctacagctct tgatataact ggtatagcag tttactttat aataacaacg
    19501 gcattattgt caaaaattgt ttaataactt tatgtatgat ttatcctctt cttactaaaa
    19561 atataagtat aaatgtaaga agggggtttt ttaaaaatga acaatatcag ttgggaagtt
    19621 tttaaaaaaa caggtagtat agatgcatat ttatatttct gtgattgcaa aaacatacat
    19681 gaagaaattg aagaagtaag ggagaaaaaa gaagacgatg ataatcctga acacccaggg
    19741 gatagtactt aaagctataa ggtataagga aagtgacata atacttacac tatttacaag
    19801 aaagcttgga aaagtatctg caatagcaaa aggagctaaa aaaaataaga gctctttgct
    19861 ttcatcatca caattatttt cttatagcaa ttttacctta aaaaaacaag gtaatatgta
    19921 caaggttact caaagtgaaa taataaaaag tttttataat atatcctatg atatagaggc
    19981 attttcttat gctacatata ttacaaaatt agttgaaaat tctatacttg aaaatcagac
    20041 taataatagg ctatttatat tacttgcgca gactctgtat ttgtatacac aggataatac
    20101 agacaatagg tttataacag cagcattcga gttaaaattt ttagactata taggatttaa
    20161 acctatagta aataaatgca ttaattgtga atgtaaaatt ttacaaaatt ctgtgtttaa
    20221 tatctacgaa ggtggtatac tatgtagtaa gtgtagtaaa ctttttgata ataatataaa
    20281 attagactta acaactataa gtcttatgga atatgtatta agaaatgata ttttaacatg
    20341 tagtaaagct aaagtatcaa aatatataac acatgaactt gagaatattc tagacaagta
    20401 tttgaaagtt tatgttgata atattaattt taaatcatta cacgtattgc aaagtgtaaa
    20461 aaataataag ggagtggata atgatgagta gtaatataac tattgaaatg gttgattcag
    20521 tctttgaaag agttccagga gcaacttatg cagaagcaaa agaagcatta gtaatgtgca
    20581 atggagatgt aattgaggca gtaatatatt tagaaaataa aaaaaatact tgtaattgta
    20641 aagcaaaatc tgctaaagaa actatggaag aagtatttgg caaagatgga gaaaatataa
    20701 agtttcaatt aaaagagctt cttagaaaaa gtagtgtagt gagaataata gttgaaaaag
    20761 atggaaaaac tatgatgaac attcctttaa cagttggagt tgttggatta gcttttgtac
    20821 cattagctac tttagttgga ttatcagctg ctgttataag taaatatcgt ataaaagttc
    20881 aaaatgaaga agatggagaa attgtagatt taggtgaatt gaatgaagaa aaacttcacg
    20941 ttttgaagaa tatgataact aatgcagcaa aagatgtgaa ggatgtagta gttgacaaaa
    21001 aagaagatga caaagatgta actgctgacc ttatgaaaga agaatctgaa aagaataaag
    21061 aagacgaaga cgaaaataaa tagatataaa gtgactttta aaaaatgaag agaagataat
    21121 tataagaatt atcactaaca ttgacaaatt ttatagaatt tgatattcta aaagttgtag
    21181 aagaactatt aaaatagcct ttcgtgtaga cgagaggctt tattgttata ggagggattt
    21241 ttatgaattt tcaagagatg atactggcgt tgcaaaaata ctggtcaaag caaggttgta
    21301 tcatgatgca accttatgat attgaaaaag gtgcaggaac tatgaatcct aatacttttt
    21361 taagatcatt aggacctgag ccttggcaag tatgctatgt agagccttca agaagaccag
    21421 cagatggaag atatggtgaa aacccaaaca gactatatca acatcatcaa ttccaagtta
    21481 tactaaaacc ttctccagat aatatacaag aattatattt ggaaagttta aaagagattg
    21541 gaattgaccc aagtgagcat gacataagat ttgttgaaga taactgggaa gctgctacag
    21601 ttggtgcttg gggacttgga tgggaagttt ggctagatgg tatggaaata actcaattta
    21661 cgtattttca acaagtaggt aatattgaat gtgagcttga gacaggagaa attacatatg
    21721 gattagaaag attagctatg tacattcaag aagttgatag tgtttatgat ttaaaatgga
    21781 atgataaaat aacttatggt gaagtattta ataaagctga gtatgaaaac tcaatgtacg
    21841 catttgaatt atgtgatgca gatatgttgt ttaatctgtt tgatatatat gaaaaagaag
    21901 cattaagact tatggaaaat ggccttgtaa taccttcata tgactatgtg ttaaaatgct
    21961 ctcatgcatt taatacattg gacgcaagag gtgcaatagg agttagtcaa agagcttcat
    22021 ttataggtag agttagaaat atggcaaaga ctgttgcaga aacttttgta aaacaaagag
    22081 aagaaatggg attccctctt ttaaaggacg gtgacaaata atgaaaaatt atcttttatt
    22141 tgaaatcggt gttgaagaaa tgccatcaag atttgtaggc agtacactag aacaattaaa
    22201 aaacaatctt agtaaattat ttgatgaaaa tagaatagct tttgataaaa taaatgcgta
    22261 tgggacacca agaagactta ctttagtagt cgaaggattg agtgatagac aaagtgattt
    22321 agaagaggaa ataaagggtc ctgctaagaa aattgctgtt gatgcagaaa ataatttaac
    22381 aaaaccagca ttaggattta taaaaagcaa aggattaagc gaatctgaca tatattttaa
    22441 acaagttgga aaagaagaat atatatttgc gactataaag caagatggaa aagaaacttc
    22501 agaagtacta aaagacatat taccagagac tataaaatct gtagtattcc caaaagctat
    22561 gcgttgggga ggaaaaaatc ttaaatttgc aagacctata agatggatag ttgcattgtt
    22621 aaatgataaa gtattagagt ttgatttaga aggaataata tcttctaatg taacaaaagg
    22681 tcatagattt ttaggtgaaa gtacaataga agttgattct atagaagatt attttgaaaa
    22741 attagaaaaa aattatatag ttttagacca aaacaagaga aaagaaatga taagaaaaca
    22801 atgtatagaa gtagcaaatt ctttaggtgg agaagttgaa tttgatgaag atttacttga
    22861 agaggtaact tatttagttg aatatccaac tgctttttat ggagaatttg atgtggatta
    22921 tataaaacta ccaaaagaag ttgttataac tcctatgaaa caacatcaaa gatattttcc
    22981 agtacttaaa gaaggaaaac tgttacctaa ctttatagct gttagaaatg gtgattctta
    23041 cagaatagac aatgttaaag ctggaaatga aaaagtttta gaagcaagac ttgcagatgc
    23101 tttatttttc tatagagaag atactaagag aaacttagaa agctacatag aaaaattaaa
    23161 aactgttgta ttccaagtaa aactaggaac tatttacgac aaaactttaa gacttgaaaa
    23221 attaagtgct gatatacttg atagtttagg tttagtagat gaaaaggcag atactataag
    23281 ggctgctaaa ttatctaagg cagatttagt tactggaatg gtaggagaat ttgatgaatt
    23341 acaaggtttt atgggaaaag aatacgcaaa agttggagga gagaatgatg ttgtaagtga
    23401 agctattttt gaacattatc ttccaagatt tgcaggagat atacttccta agactaatgc
    23461 aggggttgcc ttatcaatag ctgataagtt ggattcaata gcaggtttct ttgctatagg
    23521 aatacaacca acaggttctc aagacccata tgctttaaga agacaagctt taggtatact
    23581 tagtatactt atggacagaa aagttgctgt ggatataaaa gtacttattg aacatgcttt
    23641 agataattat tctgaattag attttaataa agaagaagtt gttgagcaaa taatgaattt
    23701 cttcaatgag agagtaaaaa atctatttaa agatttagga ataagatatg atgttgttga
    23761 tgctgtttta agttctgata taaatgatgt ttctgatatg catatgagag ctttagagct
    23821 taataattgg ctagaaaaag atgaattagt ggagatgctt actgcattta atagagtttc
    23881 tactctagct caaaaagcag aaagtgataa aattgatgaa tctttattaa aagaagatgc
    23941 tgaaattaga ctttacaatg aatttaaaca agtaaaggta aaagttaatg aattattaaa
    24001 ggataagaaa tatggtattg ctttagatga atttgcatct ttaagaccag tagttgataa
    24061 tttatttgat acagttatgg ttatggataa tgatgaagct attaagaaca atagacttgc
    24121 tttacttaaa caagtatatg atactatgct agaaatttgt gatttatcta aaattgttta
    24181 taaataaaat ttaaactatt tataagaaat aaggctgtct caaaatagag atagtcttat
    24241 ttttcattgt tgagatgatg aaaatatatt tgatttcata gaaaaagagt gtctcataaa
    24301 ctaaaaagtt cattttgaga cacccatgct ttttgtaagt aaatataata aattatattt
    24361 tatacttatc agttattttg tttaaaatat tatcatttga atttacccat tcataaaaat
    24421 tgcctgatga tgcgtccgat attttataaa gcatttcagg aatatcacta gctttaaaat
    24481 ctccaagtga ttttcccaat ctagtcttac ctatttcaaa actgcctcca acaaataatt
    24541 caaatgcatc tgtagatata ccatcaacat tcttcttttt accacataaa cctatgctac
    24601 caacttgatg aacaccacat gaatttaaac atcctgatat atataactta ggtgctttat
    24661 ttaaaatttc ttcattattt tgtaatctaa aataatctat gatttcatgt aacattttct
    24721 gactattttg tatgcccatt tgacaaattg gaacacctat gcaagatata ctttgttcta
    24781 attgagaatt acaactaata gttttactta tttctaatat tctttttgct tcatttccat
    24841 ttaaatttaa aatatataat ccctctgtca ttcctagtct aatcatagga ttttcaacat
    24901 tatctaattc tttaagtaaa gtacttaaat cctttgttaa caaaatacct ccaactggat
    24961 gtatatatac agtgtataaa ccatcctgtt tttgaggtat tagtctagaa tcttgtatat
    25021 ctattttaac tccaggttta gagtaatcta tttcttcaat attgagttca agactaccat
    25081 tttctttttc taatttatag tattctttaa atttttctaa gaatagttct tcaccaagtg
    25141 attcgaccat atatctaacc ctagctctgt ttttattttc ataatttcca taatccataa
    25201 agaatttaat cataccttca acacaatata aggcgtcttt cggctttata agttcatcaa
    25261 gttctaaagc aactttgggt tgtttaccta gacctccacc taagaaaact ctgaaatatg
    25321 gttcatcatt tttcaaagta gcaacaaatc ctaagtcttg tatactacaa tgagcagtat
    25381 ctgtgtaaca attagaataa gaaactttta attttctagg taaatggtat gtagtagcat
    25441 ttttgataaa atatttatca gtagcaactg catatggagt aacatcaaag acttcactag
    25501 ggtcaactcc agataatgga gataatccga catttctagg ataattacca ccaccacctc
    25561 tagtgaatat atcatttttt attccttctt ccatgatatc aacaattgaa tttatagata
    25621 aatcgtgtaa ttgtatagct tgtctagttg ttaagtgtat cttatctaag ttgtgtttaa
    25681 tagctatttc atagatttta ttaagttgat actgagataa aactccagca gatactctaa
    25741 gtcttatcat aaaagatttt ttgtctcttt gagcataaac accatatcca ccagaaaatc
    25801 ccttatattg cattttattt atttctccat ttaaaaattt tgaagaatat tctttaaaat
    25861 taggtagttc atttaacaat atctttttaa aattatccat tatttcacct cattgttagt
    25921 cttgtataaa aatatatcta ttgtataata gatatatata ttattatcta gtcaggataa
    25981 aaatataaca gaataattct attaaaagta agaataataa ggacatagtg agtattttgt
    26041 ttttatttta caaattaagt ataatatttt tgttttataa aatataatta agataaatag
    26101 ataattttgt aattttaata tataatattt agtagagtgt gggagtagca cttaatattt
    26161 gcaggaggag tttttgtata tgggagaaat aataaatgca ttagatagag ccctagacat
    26221 aattttgctg ttatatcatg aaaagagaga aatgggaata actgaaataa gtaaggctat
    26281 gggagtatat aaaagtactg tacatagaac tttggttact cttgaaaaca aaggttttgt
    26341 tatacaaaat gcagagaatg gtaagtattg gctaggaata aatttatatg caataggtat
    26401 ggttgtggga gaaaaaatgt cattgactga aatagtaaaa ccatatacga aaaaattaaa
    26461 tcaagaattt aacgaagtag ttaatgtatc tatattagaa gaaagagctc aagattctcc
    26521 aagaagtatc ataatacata aagagtatgg ttcaaatcaa ttactttcag taaatccatc
    26581 cgttggttca agtagtgaat gttattgttc agcagttggt aaatgtctta tggcatttaa
    26641 tgattctata gattttgaaa aatatagaaa aacaccaata cacaagtata cagaacatac
    26701 aattgataat tgggatgata tgatgctttt tttagcaaaa ataaaagaac aaggatatgc
    26761 tatagatgat gaagaactag aacatggact aacttgtatt ggagcaccta tcttagataa
    26821 aaataataaa gctattgcag ccataagttt gtcaggtcca actataagaa tgagagaagg
    26881 ggactttgag tataagatta aaagagttat agaaactgca aaaagtatat ctgaactttt
    26941 tagatgattt ttaatctcga taataaaaat aggctatatt taaattgtta tttgtaaata
    27001 tcagtttaaa tgtagcctat ttttctttaa tctaaaatta aatattctta aaatttaaaa
    27061 tgttattgaa atatataagc gaatgtgtta tcttttaatt aagcaataaa aatagaacaa
    27121 ggttctatac tatagaacaa aattgatgca tatatagtat ttatacaaaa tataaatatt
    27181 atatatttaa aattaagtaa aaatattaca taaaggggag atttgagaaa tggaaaaagt
    27241 caagttgagt gttgaaagat gtaagggatg ctatctttgt attgaagctt gcaaaaaaga
    27301 tgcaataagt ctttctgact acacaaataa aaaaggcttt gtaactatta aagtcgatga
    27361 agaaaaatgt attcaatgtg gcgcttgtta tacaatgtgc cctgatttag tatttgagat
    27421 attataaatt atttttatta gaggggagaa aaggtatgca aagtttatta attaaaggaa
    27481 atgaagcttt agcagaaggt gctatacgtg gtggctgcag attttatgca ggttatccaa
    27541 taacaccaca atcagaaata ttagaaatga tggcatggag acaaaaagaa gttggaggag
    27601 tatttatcca aggtgaatca gaaatagcca gtgtaaatat ggttatggga gcaagtgcac
    27661 ttggagcaag agctatgact tcttcatctg gtccaggatt ttcattaaaa caagaaggta
    27721 ttgcatattg ggtatcagct ggtttgccag cagttgtagc atgtgtgcaa agatatggta
    27781 taggtgatgg atttatagga gctggtcaag ataattattg gcaagcagta aaaggcggtg
    27841 gaaatggaga ttatcattta atcgtgttag cgcctaacag tgttcaagaa aatatggata
    27901 tggcatatga atcttttgaa ttggctgaaa aatatatgca tccagtactt atactttctg
    27961 atgctacaat aggtcaaatg gtagaacctt gtatagcacc accaatgaaa gaatacgata
    28021 tggataaagc tccatgggct gcaaaaggaa cacctgtagg agaaaaaaat aaagtaataa
    28081 ctgatataac atattttgaa gaccaagata catggcaaaa agggtatata gaaaaaatga
    28141 gaaaagttca agaagatgaa caaagatggg aagagataga aatagaagat gctgaagtgg
    28201 tatttgtggc atacggtatt tcatcaagaa tagcagaagg tgcagttaga gatgctagag
    28261 caaaaggaat gaaattaggt ctaataagac caaaaacatt gtggccttac ccaagaaaag
    28321 catttaagaa attaagtgat tcattgaagg gacttgtaac agttgaaatg aacatgatgg
    28381 cacagatgaa ggaagatgta attatagcat gtgataatag atttccagta tattcagtag
    28441 caacagctca atatatggca gaagtaaatc aattaataga ttttgctcaa cagattatag
    28501 atggaaaagc taagcaagag gaggtatttt aattatggct aagaaagcac cagcaataat
    28561 gatgtgtgaa aatatgtttt gtggaggctg tggacactct atagtcgata gaattattgg
    28621 agaagtatta acagaaatgg atatatgtca agatacaatt atatgttcag atataggttg
    28681 taatcatatg tttcaattta gtatgaatgt agatgttgtc gttccacctc atggtaaaat
    28741 gggtgcagca atggcagcta tgaaaagagt tagaccagat aaatatatat ttacaatagc
    28801 tggagatggt ggagcttatg cgatagggct tggggaaact atgtcagctg ctactagaaa
    28861 tgataatgtt atattccttg ttatgaacaa taatatgttt gcaatgacag gagggcaaat
    28921 ggctccaact actatagaag gtcaatatac agcttctact ccagcgggta gaaattacgc
    28981 agatcatggt tcaacatttg atgttgtaaa agttatggga aatttagata tagcatatct
    29041 agcaagagga acaataacta accctaaaga aataaataca acaaaaagat acatcaaaaa
    29101 agctctagaa aaacaaaaag aaaagaaagg tttttgctta atagaagtgc ttgtaccatg
    29161 tccaacaaac tggggattaa agccagtaga tgctatgaag agaattgaaa atgaacttgt
    29221 tccaaagtat ccacttggag aatttattga ttagggagga aataatatgt tgaatgaaat
    29281 tatatgtgct ggttttggag gacagggagt tcttacagca ggaaaaatta taatgtatgt
    29341 tgcatataag gcagaaaaga aaattacatg gttcccatct tatggaaatg agatgagggg
    29401 aggaagtgca aattgcaatg ttgttgtaag tgatgaaaaa attgcaaatc cttgtgtaaa
    29461 aaatccagat atactattgg gaatgaacag cgtatcagta gataagtatg aaagtttaat
    29521 gaaaaaaaat ggttatatgt ttgtcaatga atcactagta agtaaggata gaaagtacag
    29581 agaagacatt aatgttataa aagtacctgt tactgaaata gcacaaaatt taggcaatga
    29641 aaaagctgct aacatttgca tgttaggtgc tatggtaaag agtttggatt tatttacaaa
    29701 agaagaattt gaaagtggta tgtgtgaata ctttgaaaat caaggcaaag gtaaattcaa
    29761 ttctaaaaat gtagaagcat ttgaagcagg atacgactac gaatgaatat tttaaatatt
    29821 taaaataatt attgaaatac ttatttttat atgttagtat ttaattaata aaatatagaa
    29881 cgtcgttctg gacaatagga cgaagtcttt aggccggaat acaagaacga cgttctgtat
    29941 agtagaacta gggggtgtta aattgaaagt tttagtaata aatcctggtg gtacatccac
    30001 aaaaatttct gttttccaag atgaaaatga aattttgaaa aaaaatataa cccatacaag
    30061 agaagattta aaaaattttt ctaaagtatt tgatgaatat gattatagaa aacaactgat
    30121 agtagatatt ttaagtagtg agaatcatag tataaattct tttgacgcag tagttggtcg
    30181 tggtggactt atgaaggcta taaaaggtgg tacctatact gtgagtgaag aaatgattga
    30241 ggatatgaga aatgaaataa atggggaaca tgcatcaaat ttaggtgcat tattagctaa
    30301 aactatagct gatgaaatag gagtacaatc atttgtagta gacccagttt ctgtagatga
    30361 atttgatgat gtatcgagga ttacaggaat atcagacata gaaaaagcaa gttggttaca
    30421 tgcattaaac cataaagctg tttcaagaaa aattgcacaa aaactagggg gaaaatacga
    30481 ggattataac tttattgtag ctcatttagg ttcaggaata tcgattgtag cacataaaca
    30541 aggcaaaatg attgatggaa gtggtggtag gtctgatggt ccattttctc cagaaaggag
    30601 tggtggatta cttgcatatc ctcttataga attgtgttac tccggaaaat atactcatga
    30661 tgaaatggtc gcaaaagtaa gttcgattgg gggaatgtac gattatttag gaacaaagga
    30721 catgatagaa attgaaaaca gaatcaagga gggtgatgaa aaggcaaaac ttataatgga
    30781 tgcatttatt tatcaagtag caaaagaaat ttctatgtat ggagcttcac tttcagggaa
    30841 tgtagataga gttatcttga caggagggat atctcactca gaaatggtag tcaaaggagt
    30901 agcagaaaaa gtttcatatc tagctccaat agagatagta ccaggagaga tggaaatgga
    30961 ggcacttgct ctaggagcac ttagagtact taatggagaa gaaatagcaa aaacttatta
    31021 atatttagga gaaagagtat gttaaaagat tttaataatt tattagaaga ggctaaaaag
    31081 cttgaacctg ttattgtaag tgttgctgtt gcagaagaca aagaagtatt aaaggctgtg
    31141 aaatcagcaa cagatttaaa cattataaag ccaattcttg taggagacaa ggataaaata
    31201 gaaagtatat caagagaaat tggattaaaa ggtcatgaaa taataggttg tcaaaatccc
    31261 gatgaatcag ttaaaaaagc tgtagagata gttaaaaata aggaagctaa agttcttatg
    31321 aagggattag taaatacaag tgtctatatg agagctatat taaataaaga aacgggtctt
    31381 agaacaggaa gattattaag tttgcttgca gtatatgaat tacctcaata tcataaactt
    31441 ctttattgta ctgatagtgg aataaatgta tcgccaaact taaatcaaaa aaaagatata
    31501 atgacaaatg cacttcttac tatgaaaagt ataggcatgg aaaatcctaa aactgcgata
    31561 cttactgcaa atgaaatggt agaccctaag atacaatcta cagtggatgc caacctactt
    31621 gtagagatgg aaaaatcggg agaaatacca aagtgtatag tggaaggtcc aatagcattt
    31681 gatgtagcat ttgatgcaca tgcagcagaa cataaaggta ttaaaagtaa tattgctggt
    31741 gatgttgatt tattagtatt tccaaatata gaagcaggga atatattagg aaagtcatgg
    31801 cttagattta ataaagctaa gtgggcaggt atagttttag gagcaacaaa tccgataatt
    31861 ttaggttcac gttctgacac agctgaaata aaaataaatt caatagcatt agcgtgccta
    31921 gcatcacaat aagaaattat tattaaggag gagaaagtat tatgaaatat gcaaaaagaa
    31981 tggatatggt aaaagcttca gctataagag cttctcaaaa gaaaatagct cagaaggtgg
    32041 caagtggtgg aactgttata tcttttgctg cagggcttcc tgacccaaat ttatttccaa
    32101 tagaagagat aagtgaaata actcaaaaag tacttaatga aaggggaaaa tttgccctag
    32161 catatggacc tactaaaggt gaagatgagt tgttagatct attagtaaaa agaatgaaag
    32221 aagaagaaaa tattgataca aaagcagaaa atattataat aactactggg tcacaacaag
    32281 ggatagcatt aagtgctatg attttactag aaaaaggaga tatagtagtt acagaaaatc
    32341 caagttactt aggtgcaata aatgcattta gaccttatga atgtgatttt ttaggagtag
    32401 atactgatga tgaaggaatg gtaacagatg atttagataa attattatct gaaaattcta
    32461 acataaaggt aatctatgta ataccaacat ttcaaaatcc tacaggaaaa acatggtctt
    32521 tacaaagaag aaaagatttt atggaagtcg tgaataagta tgatgtcata gtaatagaag
    32581 ataatcctta tggagaaata agatttacac cagagcctct acctacactt aaatctcttg
    32641 atactaaaga taaagtatta tacttaggtt cattttcaaa agtattatgt ccagggttta
    32701 gagttgcttg gatgtgtggt aatgtagaca tcatagataa ggcagaactt ttaaaacaag
    32761 gtatagattt acagtcaaat caattttcgc aagtacaagt aatagaattt ttaaagaaat
    32821 acaacttaaa tgaacatata gaaaaaataa gagcagagta taagaaaaga tgtctgctaa
    32881 tgttagatgc tatgaagaaa catttcccag aggaagctaa atatacagaa ccagatggag
    32941 gaatgtttat atgggttgaa cttcctgaaa gtataaatgt agatgattta ttggataaag
    33001 ccattgatgc tggagttgct tatgtttctg gagagtcctt ctttgcaaat aatggtccaa
    33061 aaaatactat gagacttaac tatacaacaa tgtcagaaga acaaatcgtt aaaggtgttg
    33121 agatacttgc agaagttata aaaaaagaac tcaattatgc ttgtgaataa atataaaaag
    33181 tgagggttta aatatgggag aaaaagaaaa cggtataaag tttggaaagt taatgtttca
    33241 acttgttatt atagcaatat cttgggaatt agtttatatt ataccattta tccaatatac
    33301 attatatgat ccaatcctaa aagcattaga atgtacaaac actcaactag gttttctact
    33361 tacaatttat gggttaggta atatatttgg agctcctata ggaggctggt tagcagatag
    33421 atttgattat agaaagataa ttttaggttc tgtatttctt aatggagtag tatcattttt
    33481 atttgcattt aatatgaact atccatttgc agttgctact tgggtaggtt gtgcaatcac
    33541 atcattagtt atgaactatc cttctatggt aaaaatactt agagttatag gaaaagataa
    33601 tcaaggtaaa gtttatggat tcaatgaagc tatggttgga gtttctggcg tagtaatggg
    33661 agcaatattc ttatatattt atacatgctt ttcaactcct gtattgggta tgagatgggt
    33721 aatgatatct ttaggaatat tatcaatagt gatgtgtcca gtattatggt ttgtaattaa
    33781 agatgtagat atcagagaag acaaagaaga agaagcaagt gaaaaaatgt ctgctggcga
    33841 ttttatgaca gtactaaaat caccaaacac ttggctagta ggaataagca tattctgtgt
    33901 atattcattc acagttacaa tgtcttattt tacaccatat ataacatcag tacttggagg
    33961 ttctgtggca ttgtctgggg cattagcaat aataagacaa catggtctaa aattgtttgg
    34021 agcaccattt ggaggatatt gtgcagataa agtaaaatca cctacaaaag tactactacc
    34081 aatatatgta tttggtatag cagtaataat tttattccta gtactacctg catctactcc
    34141 aatgactatc tttatagctt taacatttgt agtaggtata ttaggttaca tgggaaaagg
    34201 tatatactat gcagttcaag atgaagtaaa agttccagtt aaatattcag caactactat
    34261 aggtatagct gcagctttag gattttcacc agatgtattc caatttgcat taataggaca
    34321 ctggatagat acttatggca ataaaggtta cacatataca tttatattcc agatagcaat
    34381 tctaataatt ggtatactat cttgtttata tatattaaaa gttaagaaga aaagagaaag
    34441 taagttagaa gcataaatat tgtaaaaata ccaaattatt aagttttata gcaatactta
    34501 aaatttaaag tgcaacacac caaaatgtta tcaagttttc taggttttag tataaagtta
    34561 cccctaaagg tctggaattg agtagcaatt atcatctatt tatatattta cataaaccta
    34621 tttaatttaa attaaaagaa tttcaaaaat aggtggtaat tgtactcaat tttacacctc
    34681 ataataaata cgaaaattta aaatctaact atatactatt caattatata ttaataattg
    34741 tatatggtac tatttatata gtaacagttt caggcttttc attacatgta ttccaattta
    34801 tattgtttga ttatttgtca aatacatatc gttaaaaagt ttataaattt acgtttatat
    34861 atcgattaat attctttgtt ttagagatag atacttatag cttgtggatt aaaaataata
    34921 taaatagaag atagattttt tagatatatt gagttgaaga ttatatagaa tgttgaaaca
    34981 tagctattta aattattgtc tataaatata aaatgaatgg tggtgagtta agtgaccgat
    35041 ttagttaaca ccattaatgg aattgtttgg tcacctgtat tagttgcaat gtgtttgtgt
    35101 ataggattat atttttcaat gaaattaaga ttttttcaaa taaaagatgt aaaagaaatg
    35161 tttactcttt tgcttgaagg agatagctct catgttggaa tatcttcgtt ccaaggtttt
    35221 gctacatcta tggcaggaag gataggaact ggaaatatag ctggtgtagc tgtagcgata
    35281 gcaatgggag ggccaggagc attagtgtgg atgtgcataa tggctacatt agggtcggca
    35341 actgcatttg tagaatctac tttagctcag atatataaag atgagcatga tggacagtac
    35401 aggggaggtc ctccatatta ctttgaaaaa ggtcttggtt ggaaaaaatg ttcaatttta
    35461 ttttcagtgg ttgcaatcat aagttataca ttttttatgc ctggcacaca atcaaatact
    35521 ctatatctag caatgaatca agcatttgga ttttcaaaaa tgacaatagc agttacatct
    35581 tctattgcac taggtataat aatttttgga ggaattaaaa gaattggttt atttgctgag
    35641 aaagtagtac ctataatggg tggagcatac cttattatga cattggtaat aatcatagca
    35701 aatataaatc tagttccagg agctattatg acaatgttta aatctgcatt tggatttggt
    35761 tcggtttttg gagggatgtt aggttcagca atatcttggg gagtaaaaag aggtatatac
    35821 tcaaatgaag cgggagaagg ttctggaact tatggtgcag cagcagcaga agtgagtcac
    35881 ccagccaaac aaggattagt ccaagctttt tctgtttatg tagacacact attaatatgt
    35941 atggctacag gtattatgat tataataaca ggaatgtata atgttacaga tgtttacaca
    36001 acattaccag gtgtagaacc aggtccacaa tatgtacaga gtgcaataaa tacattacat
    36061 cctcaattag gtgatatttt tatagcaata gttttactac tatttacatt tacaacatta
    36121 ttagccttct catacatgat ggagaccaac gtatcttact tagtgactag aattaaggca
    36181 gaaaaacatg aaaagcacat aattaatgtc tgtagattta tgcttataat atgtgcagca
    36241 atgtgttgct ttatagaacc acttacaagt tgggcacttg gtgatatatg tataggtgta
    36301 ttaacatata taaacttagt tggtatattt acactaaaaa aaccagctat aaaagcatat
    36361 tacgattatc aaaggcagaa aaagatggga gtaaaaagga gcgatagaac ttttaatcct
    36421 atagaattag atataagaaa tgctgagttt tgggaaaata gatacttaaa agaggataag
    36481 catgagttaa attaaagtta taataaaata taaaaattac taaataaatt tagtgggagg
    36541 taagattggt atgaaagaaa gaataataca agtggctgat tcaaagaaag aaaaaattct
    36601 agggctatgt caatctttat atgatgaacc agaaattgct ttgcaagaat ataaatctgc
    36661 taaaaaaata tcagaatttc taagagaaga gggttttgat gttgaggaaa acttggcagg
    36721 tatggctaca gcttttaaag caaccaagaa aaatggagat ggtcctaaaa tagcatttat
    36781 agcagaatat gatgctcttc caggcaatgg acatgcatgt ggacatcatt taattgcatc
    36841 tatgggagtt ggagctggaa tagctctttc aagtatactg gatacttaca agggagaagt
    36901 aagtataatt ggaacacctg ctgaagaaac tggagatgga aagccttact tgatagaaca
    36961 tggtgtattt gatggctatg atgcagcaat gatgatacat ccaaattcaa aaacttgtgt
    37021 aacacctgaa attatagcaa taggaggtct agatttcata tttactggaa aagcatctca
    37081 tgcaggagct aagccatata acggaataaa tgctttagat gcagtagtac tactatataa
    37141 taatataaat gcactaagac aacaattagt agatggaacg agaatacatg gtattatact
    37201 agaagcagga actgctgcaa atgtaatacc tgatatggga aaagtaagat tagagataag
    37261 ggcaaaagaa caaaattact ttgatgaagt agtagaaaaa gtgaaaaatt gtgctagagg
    37321 tgctgcaata gccactggat gtgaacttga attttatcac tttgaaccaa cttgccaagg
    37381 attaaatgaa aataaagttt tggtagacat atttacaaag attatggagg aatttggaat
    37441 atatgaagat gaacaagtaa tgttaggttc aacagatatg ggaaatttaa gtcaaataat
    37501 gccatgtata catcctttga tgaagttttc tgaaaatgga gaagaactac atacaaaaga
    37561 atttttggag gcttctataa attcatatgc aaaagataga gtaattgatg gtataaaaat
    37621 attagcttta acaggattta atttatttga aaatccagag ttattgaaaa aaatgaagga
    37681 agagtagaac ttagactatt tttaattata ctctttaaaa taagaagagt gtctcaaaat
    37741 gaacttttta gtttatgaga cactcttttt tgcatgaaat taaatatatt tttattattt
    37801 taacaatgaa aaagaggctt atctgttttt atataacctt ttttaacttg tagcacaaaa
    37861 tatcatgaat gattacaatt cgaatgataa aatcgattat aagatatata ggaggggtac
    37921 atatggaaac tttagaagta agaaatattg aattgagtgg tacaaattat gagataggat
    37981 acagactagg agaattggtt gctaatatgc cagagataat agaaggtcag attaataaat
    38041 ctaatctggt aagcaaaaaa gaagaaaaag agatgataga gttgtttgat aaatattgtc
    38101 caggtcttaa tgaagaattg caaggttttg ctgatgcaat tcaagtgaat tgtaatcaaa
    38161 ttttatatta tactatgact tatcttaaac caggttgtag tcaagttgca ttaacaccag
    38221 aattaactga aaatgggcat gtactatttg cacgtaattt cgatttctca cacaatatgg
    38281 aggattttgt tttatgtaaa actaaagtaa atggaaaata tgcacatata ggtactaccg
    38341 ttatgcaatt tggaagaggt gaaggtatga atgaatgtgg attaggtgta agtcagtcat
    38401 catgtggtat tcctgtaggc aattcagatg gtttgagaaa accagcaatt gtaggtttac
    38461 agttttgggc tgttattaga tatttgttag aaaattgcaa agatgtagat gaagcattag
    38521 aatatttaaa agatatgcca attgcataca atattaattt attgcttgca gataagtctg
    38581 gaaacattgc acttgtggag actttagatg ggaaaaaaga agtaaatata atcaacagtt
    38641 tagaaagtaa gagagaatat tttttacact ctactaatca tacacatatt gataaacttc
    38701 ataaattaga accacaatca atgaaaaatt ctattcatag atataaatta attaaagaat
    38761 atataaataa ggctaaaaaa attggagaga aagagttaat gaatttattg tctttaaagt
    38821 atccaaatgg cttatcttgt aattactata atgatttttt tggaacttta aaaagtatag
    38881 ttatggattt aaatatagga aagtttaata ttttatgggg tggactagag aataaatggg
    38941 agagctacta tttaaaaaat gacattaaat ctataacaca gaggataaac ataaatattg
    39001 aaaaagcacc atctgacttt tttgatttca ttgaataaaa aatgtattta attattctaa
    39061 ataattaggc tatataaatc aactaaaagt ttttatttat cgtattgaat tagttataat
    39121 attttagtat agtaaaaaat agtgctattt tacatattta aagagtttaa gtagaagaga
    39181 ggtgccatat tggattactt aaagtatttt gaaaaagctg taatgtatat tgagaataat
    39241 ttacatgaaa atataactgt aaatgatgtg gcaaaagaaa ctggatattc ttattatcat
    39301 ttaacacgct tatttaagtc tatgtttggt gagagtgtag gaagttatat aaaaaaaaga
    39361 cgattagtaa gtagtacaaa agaactttta tatagtgata aaaaagtgat agatatagct
    39421 attggtagtg gatttgaatc ttcggaagca tttagcagag catttaaatc tatatacaag
    39481 gttagcccta tagaatatag aaaaaataga attgatgtat ttgttggtaa aaagaaaaaa
    39541 ttagagttag attttatgag acatcttgtt ggaaatataa cgattaaacc agttataaaa
    39601 gaaatagaaa aaataaaggt aataggtata aaggataaag taattttaga aaataattca
    39661 ttacctgact tgtgggagaa attcagaaaa gtacatcata ttgtcccaaa tactttgcca
    39721 tcaaagagag tttttggaat ctgtgaagca atctctgaaa tacacctagt tagtgaaagt
    39781 atggaattta atgagataat aggacttgaa gttaatagtt atgatgtgat tccaaattca
    39841 tttgtttcaa aaactattaa ggaaggaaag tatgctatat ttactcatac tggtagctta
    39901 gataacttag acaaaactta tgaatatata tggggtactt ggtttttaag ttcaaaagaa
    39961 gaactggata taagagatga ttttgaagta tatgatgaac gatttttagg tcctagtaat
    40021 gtgaactcac aaattgatat ttatatacct ataaaataat ctataagaaa actaaaagat
    40081 aatttgatta agaaatagat gttcg
//
LOCUS       DAGQWR010000015        39743 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.015, whole genome shotgun sequence.
ACCESSION   DAGQWR010000015 DAGQWR010000000
VERSION     DAGQWR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.015"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..1458
                     /locus_tag="MIW95_000709"
     CDS             <1..1458
                     /locus_tag="MIW95_000709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150058.1"
                     /translation="NTTKTTLIDEVNTVKAEVTTAKNNMISEVTTAKETMQTEVTDAI
                     NAIPTKEELKGVGIEIKGSLDNISALPVNPTLSDAYFVKSATIENQIDLYVWDNTNWV
                     KVPDIKIKGENGDGLEFNWDGTRLGVRIEGQENYTYTDLKGQKGDKGDSIEFNWDDTR
                     LGIKIEGQENYSYTELKGEQGYTPTIGENGNWWINNIDTQKPARGASLRILGKLDSVD
                     KLPLDPAIGDCWIVGRNIYIYQTEWKDLGSLAGVDGKNLEFNWVGTQLGVRQQYELDY
                     KYIDLKGDNIEFNWDGTRLGVRIEGQENYTYTDLKGQKGDSIEFSWNGTELGVRIEGQ
                     EDYSYTNLKGATGNKLEFNWNGSQLGIREEGQTEYIYTELRGEQGYTPAIGENGNWFI
                     NGEDTGKASKGKVTWNELLEKPKELDYAKKETIFNSDGSITDILDSTNKTITKFNADG
                     TIVDEKYVNDILVSKVKTTFTGTKIEEIKEKIEVS"
     gene            1458..3308
                     /locus_tag="MIW95_000710"
     CDS             1458..3308
                     /locus_tag="MIW95_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBY3150059.1"
                     /translation="MSWAEIYKVNSDLQGEPLNFLSYLQDIKLNGLDSYVLFIGNARI
                     WEELYLNSLYLFSDKGIRETVYTAFSETDIDNLFNKSTKLGEQLNAFYRTDIFSLGNA
                     DNVVKGMTIEHYNSLEEKFKAGYDRYVTREQEKSTIGAWFNSTFSLDNTDLENLTTIE
                     EILANVEATNAILNNSNAIVALTMCKTSMDAVVASSNAMDLLGQYILKVTTEQQVIRA
                     IIKNEVIRDAIINSNEAMTQISSNENSVMEIFDDLEATKVLVQNQNSINKILTNNVTV
                     EKIIPNLLEMKSNLQISLNYIKTIKSNIVSSKGQILAITYNEKIFPLLKNIEKNHDNM
                     ENIRNLLQQDVEEKIKIADAILGSSIAMTAFANNSIIVNKVGDRAGIIENILSQTASL
                     NAFMESTTAINILVNKTTAFTKIANNSNAMSVIANNSNTMSVIANSSNAMSVIANSSN
                     AMASIVNSPTALDVVLARSVAMREIAKIQSAVNKITGTAVALNKVVKNSAAKSALATQ
                     NAKLQANKSAIHTILKNNSNYFREIIVSQKSDSSPTQEISSTYIGVTYCYGYNGGSGY
                     GIVYHGHDTNIEAGRANGSQDENKKFITLGGTKFTESGDGYFMYSIYQAI"
     gene            3405..3668
                     /locus_tag="MIW95_000711"
     CDS             3405..3668
                     /locus_tag="MIW95_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150060.1"
                     /translation="MEELITQLSGLGAVGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVEMGAMKTDVEEIKEDVTDIKDMLQKEGL"
     gene            3669..4475
                     /locus_tag="MIW95_000712"
     CDS             3669..4475
                     /locus_tag="MIW95_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY3150061.1"
                     /translation="MRVAVTAGHTLAGKGTGAVGYINEGKENRILMDLVVEWLKKGGA
                     TVCSGKVDKSNNYLAEQCQIANKQDVDVAVQIHFNANKTTLNPIGTETIYKTNNGKVY
                     ADRVNTKLATVFKNRGAKSDVRGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKNIDNKENSEDKKMYKHTVVYDGEVDKILATVVGWGYNDGKILICDIK
                     DYVPGQTQNLYVVGGGACNKIGSITKEKYTAIKGNDRFETLYKALDFIDR"
     gene            complement(4612..4869)
                     /locus_tag="MIW95_000713"
     CDS             complement(4612..4869)
                     /locus_tag="MIW95_000713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150062.1"
                     /translation="MKNITEQLKETIVEELYGIENNEGCHEDYIEDYETEVDFYLSNV
                     KSDTYEVYVKEYCSNNHDISISNELAFEIIDELIGKIKDNN"
     gene            complement(4915..5352)
                     /locus_tag="MIW95_000714"
     CDS             complement(4915..5352)
                     /locus_tag="MIW95_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150063.1"
                     /translation="MNAKLNKELYNLGFKIGSENVLSRNKILELNDAIYRYQDRNKAF
                     EIILKAFMKLDIPMPTEIVENLDNYEVIVNFVVGVYNGYIEQLNNFSNFISLSDASKK
                     YNKAESTLKQNIKNGKFVEGVDCRLFGKSWVFNVDSLEREYSK"
     gene            5525..5821
                     /locus_tag="MIW95_000715"
     CDS             5525..5821
                     /locus_tag="MIW95_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150064.1"
                     /translation="MKKITKKEIIEFVRDVVEEYRDWKLESCGFYIKDNELNSFISFE
                     GKGIDINVYKENYEEIIYIEDYIKDYKCKEYNLKEIDSIIYEDVNEMISNYNEK"
     gene            5864..6334
                     /locus_tag="MIW95_000716"
     CDS             5864..6334
                     /locus_tag="MIW95_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150065.1"
                     /translation="MNKQKARRFLNVIDMNIDKIEEEAIKAFKESCLIKEENDIKIFI
                     DFQGKVEAIAVSTWAKLLDDDKEINIFTLTQTPTHLDDMFGELCYVNDYEEFENWCEN
                     EDESLNWDSYKKFNKENFEEITERNIDDSSSVFLEKLREGIESCKQELQNIVEN"
     gene            complement(6510..6791)
                     /locus_tag="MIW95_000717"
     CDS             complement(6510..6791)
                     /locus_tag="MIW95_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150066.1"
                     /translation="MNVYLQIGKELSEKMTKSELNKFSQEVVNFLRYAGFDKNMKIKL
                     FQTIIEVVARKKVSLPSGSEFTEGILQSDEAEMTMNVGFIIEGLSYHSN"
     gene            complement(6935..7267)
                     /locus_tag="MIW95_000718"
     CDS             complement(6935..7267)
                     /locus_tag="MIW95_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150067.1"
                     /translation="MPSFDRDLLVDKEKPVLKNGTIVQNDSTTTDNKTLNIYNLVNKK
                     SKKVTMSATLDEELVNKLKSFSIDMNSDVSKLLSDILTQILSDVTIKEENLNIYNERN
                     RRNKTKKK"
     gene            complement(7268..8077)
                     /locus_tag="MIW95_000719"
     CDS             complement(7268..8077)
                     /locus_tag="MIW95_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBY3150068.1"
                     /translation="MADTKLITYFNIKGGIYKTTTSIMTAYELAKDKDKKILLWDLDV
                     QANLTQYVYEINHNDNTTLDILKGISANDVIVKSPNGNYTNVDLIPSDIQMARFEQEL
                     SPLPAREKFLARWYMENFNILSEYDYIICDLSPRYDLTSKNVLFLADSVIILIQDKNI
                     SSLRGADLFKQLWDVDRTYFDKEDNIKSTILVGFEKKKTQISDTFDSYLEGFNDMRDI
                     MLDTYIRKNEFIEKALLKKLSLTDYTKITKDHFSRQEFTNMLEELKVKGVL"
     gene            complement(8363..8524)
                     /locus_tag="MIW95_000720"
     CDS             complement(8363..8524)
                     /locus_tag="MIW95_000720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBY3150069.1"
                     /translation="MNINFNKSGCGSTSTRLNLPIRFLRELGVTESDRSVEVTLKGDE
                     IIIKKAKNE"
     gene            9961..12126
                     /locus_tag="MIW95_000721"
     CDS             9961..12126
                     /locus_tag="MIW95_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBY3150070.1"
                     /translation="MKNTNKNNIDKLFLEECNAERAEHEKENYSEFNRHEIYEIKKYI
                     DEKPLGIRTAIANADDRGKTLFIAPTGAGKSYSFINTLKKLKTKALFILPNASNVEQA
                     MHEYSVAGAYDKIPAKQALKGNNLVVMTWDKTEQLIDVDLSEYIIVVDEIHQTYTDAY
                     RAKAIKNLNNIMTKCKGRIDITATPTKLEFEIYDYIIEYTQTQKTKYNVKLYNDFDNK
                     NFTEIINIINKSKNSAMLMNDISTLEFIRDSVDKNADVVYADGKEENELYNRIVKNSD
                     MKGYETLLNTTTILAGVNIKNKNITDIIIVNIKDVGAIKQYVARFRNLKKANIHIFNK
                     YDEECNIYKIEWLVNKNIEKATILKEAYNKVSKHMLQFETVGINATPIRMDSNVYYCT
                     KDNCYKVDKLYIKSQVYSNYYNTRTIQSFKVLLEEYFENIEIADMKENITNEKELKEY
                     KKVAKEIKDATREILKEHKEILVGYRQIKSDSKSFKLMQYHNDMKLSSEGCLKAYKEY
                     DIHNLVKKSKSNSMLELYSNYVLDNKFSLELAWKLANTANRKRGAIFNKINTLIYREL
                     KEEYPQFLNDELIEVTKFNYIDKLFGVGTSYTKVHLQELTNDLRLILGEVWDLNTKKL
                     EIILNGIFVIENKNYRSGSVLEHIFFYKNINPNTEPGTRKRIRINNIKRYITIDDIKQ
                     ELELDKKDNSLEHAIEYTKDRMLNSLDEEEKLLLLKGLM"
     gene            complement(12775..13161)
                     /locus_tag="MIW95_000722"
     CDS             complement(12775..13161)
                     /locus_tag="MIW95_000722"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3150071.1"
                     /translation="MCEQIQEENIKDRIKEIREDAGLSQKDFGSKVKLSRSQISCYEK
                     GIRDATDRSIKDICREFNVNENWLRFGEGEKYSINEKDSLLAEVLAEITISDNIPIKE
                     IIIKLCELDEEYLDLINKLIDGLLKK"
     gene            13327..13497
                     /locus_tag="MIW95_000723"
     CDS             13327..13497
                     /locus_tag="MIW95_000723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150072.1"
                     /translation="MRAEKIKTIVRKINFLDELGLEIVNNIGDLCIKDQKNKKKNNSY
                     DLENIVSIDKMD"
     gene            13582..14001
                     /locus_tag="MIW95_000724"
     CDS             13582..14001
                     /locus_tag="MIW95_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150073.1"
                     /translation="MIKVILCNTIDEYMKYEDVIGAKDSYLMDFAVSDECSEEDAMKA
                     LDTLGNSKVRIMSEVGKMAFNFGDLIAKGMEQGKKEAPKLKRRICCDKCGASVDSSEE
                     LTSMPVLENYNNNRSVSDQLGSIDVCPDCLLEITDLL"
     gene            14078..14482
                     /locus_tag="MIW95_000725"
     CDS             14078..14482
                     /locus_tag="MIW95_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBY3150074.1"
                     /translation="MINLETLCNGETKEKIEKGFMEIFKNIQDPNTPATSTRSLTVKV
                     TLKPGKNRSHVNTQIQVIPKLASVLPSETDIIVEKDFRTGEVDANEYGNQLPGQVKLG
                     DLESKETSVTEETEVKESEEDNIRKFKSLKDL"
     gene            14505..15218
                     /locus_tag="MIW95_000726"
     CDS             14505..15218
                     /locus_tag="MIW95_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150075.1"
                     /translation="MLTDLFNRVMEVGARKIETKEVNGVTYTTDHLIPVHSKPVEIIG
                     LNTLDGLIGYIKSEFDSDAKSDRLIVHVESPKSVALYSELMASGKRNIYVDSKALLPH
                     INFGCSLDTEEFNIMLQSSFVDTEDKNLLLKVSGNVKETSVKTVQDDGVSQVVAMSTG
                     VASVEDVILPNRVKLKPYRTFAEVDQPESEFIFRVKEGITFKLIEADGGAWRLEAIKN
                     IKEYLEKKLEGIENIDIIA"
     gene            15274..15438
                     /locus_tag="MIW95_000727"
     CDS             15274..15438
                     /locus_tag="MIW95_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150076.1"
                     /translation="MYKDITKMGAIERGEYVGNVALELREENPHLSKIDLIELALKKI
                     REESEQEAGQ"
     gene            15435..15767
                     /locus_tag="MIW95_000728"
     CDS             15435..15767
                     /locus_tag="MIW95_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150077.1"
                     /translation="MKIGKYLIVSKEQMDRNIECLKEEIKIRDDKIRLHECIMSNNEE
                     LIQILEEQLKEYKSELKEYKSELDVYSLFIDCILNKETRKAIAIYNRTKSIRIKRKCL
                     NSIPVKKG"
     gene            15815..15976
                     /locus_tag="MIW95_000729"
     CDS             15815..15976
                     /locus_tag="MIW95_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150078.1"
                     /translation="MDEFVECNRCGRIINTEEDGCVKYEKENLGIIVTLNMCLDCLEE
                     LIEDAKFLI"
     gene            15999..16171
                     /locus_tag="MIW95_000730"
                     /pseudo
     CDS             15999..16171
                     /locus_tag="MIW95_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894273.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
     gene            16283..16690
                     /gene="ssb"
                     /locus_tag="MIW95_000731"
     CDS             16283..16690
                     /gene="ssb"
                     /locus_tag="MIW95_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBY3150079.1"
                     /translation="MNNVVLIGRLTKDPELKYIPGSGTAVSTFSIAVDRDYIKKDGTK
                     ETDFIPVEIMGKLAEVCANNLNKGRLVAVQGSIRVEHYEKDDEKRTYTKVHANRMKFL
                     DYKKEDNGKECKFEPGGLDPQGFQAIDDDDIPF"
     gene            16703..17065
                     /locus_tag="MIW95_000732"
     CDS             16703..17065
                     /locus_tag="MIW95_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150080.1"
                     /translation="MIKKVEKIFSGSVNPNFMEDDPTFINYLALYCCIKPVKVRGNKK
                     VLASSTKACSALKIREHQKKERKKVRLINAKTGKIKEMAIDEAENFLNVKNLYPVIWR
                     GRPTRTGWYVKDVEDEGD"
     gene            17052..17222
                     /locus_tag="MIW95_000733"
     CDS             17052..17222
                     /locus_tag="MIW95_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150081.1"
                     /translation="MKEIKYNVVYFNLKTLKFSEKSFNTLKEARAFKKEKEKKYENVE
                     IIKKTIIEKLII"
     gene            17500..17943
                     /locus_tag="MIW95_000734"
     CDS             17500..17943
                     /locus_tag="MIW95_000734"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY3150082.1"
                     /translation="MDKERIKKTGRYLNDLGDRKRRINILKKEIAILKESEKYSEINF
                     NELGFKIKTSPKGLDDMIINSEQQILLKESEIEYIEKRLKIVYSYMQELESDEREIIR
                     LRYFYDINNKMTMTKIASQVNCCRWNVYRKLDNALWKLSGMLSIF"
     gene            18111..18755
                     /locus_tag="MIW95_000735"
     CDS             18111..18755
                     /locus_tag="MIW95_000735"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBY3150083.1"
                     /translation="MKEKIKRWVDASEAIEAYEKNVEKIIDDKRRIEREALRVDPIKS
                     QKDIDKVKRYLYKKDKRYYLIFVLGINTGLRISDIVKLKVNQVYKRTHVYLKEKKTSK
                     SNNIKINESARGAINEYCCNLSQDDYLFISQKGEYLKEKTVYKVLKKAFKKCRLRGNF
                     GTHTLRKTYAWHMNKTEGLEVVQWALNHVNQSDTLKYLGMKQEALDRAVDRLNL"
     gene            19282..19893
                     /locus_tag="MIW95_000736"
     CDS             19282..19893
                     /locus_tag="MIW95_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY3150084.1"
                     /translation="MKVLGYCRCSTNEEKQDIDRQIRDIKQLAKAHGHELSNKNIFTE
                     YESGAKIDRIELNRMLDSLGEGDIIISTEVSRISRSTKQLINILELVKEKKNKLILGW
                     FVVDCTKGSLDAMTEGMLKMMGVFAEMERAMTSDRVKSGMANAKAKGKNIGRPKTSID
                     SIPNIFLKHYSKYKNKEINISEFSRLCNMSRTTIYKYIKLLED"
     gene            19921..20634
                     /locus_tag="MIW95_000737"
     CDS             19921..20634
                     /locus_tag="MIW95_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150085.1"
                     /translation="MVINIARPRNPNRDRAREIYKEKNGKIELLEISDILNEKLKNIQ
                     KWKSVDKWDAHIKRGGQPGNKNALGNNGGAPIGNKNSSDNYLCSSFKNTIPRGLLKVW
                     NSVYNDELNPLDYLWASITLQYSKIIYAINITHVKNKKDHTIDITKEDGKKYKEYQIQ
                     HSWDKELASIKAITSAFNVLTRMIKDYEELLHKNWDLATEEQKSRIENIKARTNKLTG
                     NNLEIEDIEDIEAEIYGSN"
     gene            20621..21943
                     /locus_tag="MIW95_000738"
     CDS             20621..21943
                     /locus_tag="MIW95_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBY3150086.1"
                     /translation="MAVTKKKTIPFKFGDKHKEYIKRCASNTYNIAEGAVRAGKTVDN
                     VFAFAHEIKTSKDKIHLATGSTSANAKLNIGDANGFGLEYIFRGQCHWGKFKGNECLY
                     IKGISTKHKQKIVIFAGGAKADSYKKIRGNSYGMWIATEINLHHDNTIKEAFNRIIAS
                     TNRKIFWDLNPDNPGASIYKDYIDNYMEKDKEGTLLGGYNYQHFTIHDNITVSEERKQ
                     AIISQYDINSIWYKRDILGQRCVAEGLIYEYFANNKEQFKTETVDPLMDVVVGVDFGG
                     NKSYHAFVATGITYNYKKVIALASERPSADTSPDELNNLLINFIKKVINMHGKVDCVY
                     CDSAEQVLIKGIKNAVEKENLNVSVRNALKSAINDRIRLTDLLISQGRFEYTKYSETL
                     VNALCSAVWDSKETNEDIRLDDGSSDIDSLDGFEYTIERYLKRFIRGD"
     gene            21944..23356
                     /locus_tag="MIW95_000739"
     CDS             21944..23356
                     /locus_tag="MIW95_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBY3150087.1"
                     /translation="MFDGLKNAIRGVISKLFNKSSIQKELKVDIAVSDKMSRAIDLWM
                     SMYKNKSPWTNEITKSLNLSATISSEVARLVTLELESEVVGNDFLNEQYQDVLKDIRK
                     YCEYACGAGGLVFKPYASEKKIEVDYVQATNFFPVEYNSAGDITSAIFSEIKVKGDIK
                     YTRLEYHKWTPNSYTISNYAYKSNAAKLIGYSDDLGKRIKLSDVPEWAELSEELVLEG
                     IERPLFAYFKIPQANSLENTSPLGISVFSRAVDLIKEADKQYSRILWEYEATEVAIDA
                     DSSMFRRNSSGEYEVPHGKKRLYRMLEMEDGDNKWNVFSPTIRDSSLFAGLNQLLRKI
                     EFNCGLSYGIISDAQETEKTATEIKTSKQRLYSTVKDIQKSLEDALEGLIYSIDKWSI
                     LAGFTPSLKYETTFNWDDSVIIDKDSELLAMQQDVASGLIRPELYIAKKYGVTEEEAL
                     KMMPDTGELVEDNPFDKGGM"
     gene            23357..24586
                     /locus_tag="MIW95_000740"
     CDS             23357..24586
                     /locus_tag="MIW95_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003372696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor capsid protein"
                     /protein_id="HBY3150088.1"
                     /translation="MLTPNELQEIPKYFVSLFQALEDFIIADIARRIAKVGNITDMAE
                     WQLIRAEEIGIADKVIKRKISEILGISFEAVDKLFEESAIKSIESDSALYEHAKLTPI
                     HLNSSEELKNYVSSAKEQTRSKLKNMTGTLGFCTIKKGKVISKKLTDIYIESLDLAQF
                     QVSTGVLDYKTAIKGAVKKLADSGLRFIDYETGWANRIDVATRRAVLTGVNQMSQNIN
                     NKVINDLDTDIVEVTAHSGARCEGAGIKNHKKWQGKWYSISGKSTKYPSLKAITGWGQ
                     GDGLGGWNCSHQFHAVIPGISVPAYTKEQLKNIDPPDIGYKGRTYTHYQALQYQRKIE
                     TSMRQTKRQLIAYESAGLKDEFTSASIKLQRQKQEYREFSKVAKLRLQNDRHQVMGYN
                     KSVSQKAVYAGKKGEKK"
     gene            24583..24843
                     /locus_tag="MIW95_000741"
     CDS             24583..24843
                     /locus_tag="MIW95_000741"
                     /inference="COORDINATES: protein motif:HMM:NF025245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150089.1"
                     /translation="MNKNSITQQQIDDLFNQSEKKVDVYWGKCTIMTIQLSNGFTLVG
                     HSACVDPANYDEQIGLDICEEQIKNELWKLEGYRLQCELGKL"
     gene            25003..25575
                     /locus_tag="MIW95_000742"
     CDS             25003..25575
                     /locus_tag="MIW95_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150090.1"
                     /translation="MKREFLKSLDLEDEVIEKIMSENGRDIEKYKKEVEKKKEELESK
                     NTELETANNKIKDLEKIDVESIKKEANDWKSKAEQAQKDKELIENQMSEQTYNLNLDN
                     YLSNFKFSSNLSKEAVKIKMKEKRLEYKNGAFEGADDYMKELQANDPGAFVSGNSVPK
                     VVSSSSGDAGESKVSLMEQMIAKNREMLNI"
     gene            25586..26659
                     /locus_tag="MIW95_000743"
     CDS             25586..26659
                     /locus_tag="MIW95_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage coat protein"
                     /protein_id="HBY3150091.1"
                     /translation="MSLFDSKIFNGEVFGKYVETVPNLNRDELIKSGAIRRRDDLKAM
                     FSAQSGANYATIPMLGLIDGDPVNYDGQTDIVATTTKTFSQSVIVVGRAKSWVEKDFS
                     QDVTGGVPFMENVGNQVAKYWDNIDQKTLLAVLEGIFAMTGAKNLEFVNNHTFDITKE
                     AETENQKVKAETLNSATQKACGDNKEKFALVIMHSVISTNLENLNLVARLKYTDPQGI
                     QREMKLGTWGGKLVLIDDNMPTREVAESSEGSNDGYTEYTTYVLGENSIDFEDVGAKV
                     PYEMDRAPGKNGGEDYLYSRQRKVFVPYGISFTKKAVATASPTDSELKNGANWELVND
                     SSDGSKTYIDHKAIPIARIISRG"
     gene            26663..26995
                     /locus_tag="MIW95_000744"
     CDS             26663..26995
                     /locus_tag="MIW95_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150092.1"
                     /translation="MNMYIDYEFYSTLGGEIPQGKFNKYAIRATKYIDYNTFNRIEEV
                     TEEIKIATCEIADLIYKSSLEGDKEVQSESIGSHSVTYSANNKTIEQKAYDILKMYLD
                     SDLLYRGV"
     gene            26997..27431
                     /locus_tag="MIW95_000745"
     CDS             26997..27431
                     /locus_tag="MIW95_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150093.1"
                     /translation="MFFKDNITLYNKYYDKYSDKTLYKRAYLFGVDYQGSKNIAVTDK
                     GLLSADSVKVIVPFSANARGKKYIDPFKYYELDEIEKEKFYTFKVGDIIVKEVVDFEI
                     TSVKPYTLRELQSKFDDVSIIKSVIKCDFGSMKMRHFELEAE"
     gene            27438..27785
                     /locus_tag="MIW95_000746"
     CDS             27438..27785
                     /locus_tag="MIW95_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBY3150094.1"
                     /translation="MNVSVNIDPNKIMRARGLEPGGKAQKFFTHEVRRLSDPYVPMLN
                     GVLKNTAREEVNKIKYIQPHAKKQYYENAGRGKGGTSNGGKRGKYWEKRMLADNKDGL
                     IESVAKFIGGNRE"
     gene            27782..28213
                     /locus_tag="MIW95_000747"
     CDS             27782..28213
                     /locus_tag="MIW95_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol resistance protein"
                     /protein_id="HBY3150095.1"
                     /translation="MTIIESVREFIKKCPYLDEFAKSINVEFLGEEFTSYTLETVPSE
                     TVIKRFINGDSIKQFIFIFASRECYGSDVMQNIENSQFYEQFADWIYRENLSGNLPAL
                     SGNKEAMSLEVSTPGYPVQTSIDTAQYQIQLKLKYFEKGEM"
     gene            28216..29016
                     /locus_tag="MIW95_000748"
     CDS             28216..29016
                     /locus_tag="MIW95_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150096.1"
                     /translation="MAGMETIQRYRVADYLGVLGGDGQNSWVLMGAGFNTLDENPSAQ
                     SDSKTYINDRNSTSAIKSYQTQFSFETDLIKNEEAIMELYKIGRDQATGADAERDYLR
                     VELFLPIEGKENTFRARKFRVSVEVSGFAGAGGETITVSGNLNTVGDLVHGEFNTQTR
                     TFVEDGAEAPPAESLKVLTINSVAGTSTGNTKITVTPTLESGNSYKYKTGANVAVPTL
                     DTDCSVGYTAWDGTEEITATKGNKILIVEVNANNKAKKAGITTITVKE"
     gene            29032..29394
                     /locus_tag="MIW95_000749"
     CDS             29032..29394
                     /locus_tag="MIW95_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150097.1"
                     /translation="MIINNIELEDFDIYDADEAEKVEKTISLTITETGKVEKEATKGY
                     EMIRDICNLIFKCFDDIFGEGTSSKMFRDKRNLKVCSNAFFELIEEVNKQKQEGEEFF
                     ASAISKYTPNRAQRRAKK"
     gene            29394..29999
                     /locus_tag="MIW95_000750"
     CDS             29394..29999
                     /locus_tag="MIW95_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage Gp15 family protein"
                     /protein_id="HBY3150098.1"
                     /translation="MNMLVDILPESVEIDGKEYKINTDFRISILFEMLIQDNSISDEE
                     KGENALLLYYPVIPANTTMAIDKIIWFYSCGKKENNVDDGYQGGAGSSKSQIYSYDHD
                     DEYIYSAFLGQYGIDLQDIEKLHWWKFKALFKSLKEDTEIVKIMGYRAMDISGDMPKA
                     QKDFYRKMKKIHAIPLPQNEVEKINEIEKALLNGGDISNLL"
     gene            30073..30714
                     /locus_tag="MIW95_000751"
     CDS             30073..30714
                     /locus_tag="MIW95_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBY3150099.1"
                     /translation="MGFKFRKSINLGGGLKLNINKNSVSISGGVKGARVSVNSKGKST
                     TTIGVPGTGLYYQETTQLGGGKKKNSKSAGGSESSRQAQQLLKIINDCANIVNTTKDP
                     KTFFYRYNMLLDKSYALVAIEDDLNFSGQSPSQMLDSIIAKKTDTINDFLTRYYNEMS
                     SKLKELKTEKARMNNAYKFSSLAIDYKEHLNNDNKAKLNRLYKQLLDDVKRQD"
     gene            30822..35972
                     /locus_tag="MIW95_000752"
     CDS             30822..35972
                     /locus_tag="MIW95_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBY3150100.1"
                     /translation="MADGSIIIDTLLNTEDAEKQLNNLADTMKKKAKSALAIAGIGAS
                     LGAIGKQAIDFGDEYQKAMNGFESATGNAEARAKGFGEALQQVYANNFGEDMGDISEV
                     MSLISQKLEGIDASNIQEVTESAIMMRDTFDMDIGESLNGVNSMMRQFGVSAKESYNL
                     IAQGAQEGLNQNDDLGDQLAEYSTYYAQMGFSAEEMFNMMKNGAESGVYQIDYLNDAL
                     KEFNIRTKDGSNGTKEAFEALGFNADELTKKFANGGSSAKEAFIEVTTALNNLDDNVL
                     KNQIGVQLFGTKFEDLEADAVTALTNIKGSISSSKDKLEEINKIKYNSFGEAVTGIGR
                     QMQVNLLLPIAEGLLPVLNDLANRFAQAFQNENTKNAIRSTAESIGGLIGTVVKLISL
                     ALTPLINVITFLIQHGSLVAPIITGIGVAFTSLKVAGIVRGIQKSFTDAQLAVRIFTS
                     GVTESETKLTLYQMVVGLVTKKISLAQVATNLWKGALTALGGPVGLTIVAVTALTAVV
                     VSLWKNNEAFRDAVIKIWNNIKDAGAKIFGQIAKFFTETIPQALSKAISFIKGNWQGL
                     LLMLVNPFVGAFKLIYDNCESFRNKVNSIFNKVIAVLKNSLNFIKNSWQKIGSMLITP
                     FVNGFKSIYNSCISFKNKVFSFFSNVVKGFVTFGKNIITGIVNGLLSGIGLVIDTVKN
                     IGSLVVNTFKKVLRIHSPSKVTTELGEFAGIGFANGIKNTSKDVVEATKELISAMEKN
                     LETNKSKFEKICEAITTALKNQYESQKDVQLKALDERLKVEEKASSDRLKVYEKEYNE
                     KLKYLDTETNEKTNAIQEQIDAIDKQIEEEQKAEEEKAYNEKISDLDRKLATAKNQKE
                     REKIQKEISEAQADRQKKLLDEQRQQEKNRLKEQIENIKTEASNKKEQYKEEYDNQKE
                     AESKKLELMKESNASQKEEIEKYFSELLEETNIQNEARRLLLQKNSEEIIKLLSEYNP
                     HWQDAGQSLADSLLNGVNSKKQSIQEAVKEAINLKEIIPVQEKELDRLKKKLEEYEKL
                     KEKASASGASGGNVDSTAKSSSLDSSSAKLDAGELEEYAGGIDDVKTSVEGLDLISDE
                     LVTGTVPKIGDSAGKLSESIKKGFNGIGGFFGGIEKWLRDTDKNINKWLASAKNSIGD
                     FFSKIKDKFKEGIENIGGFFGGLGNKISEGFAGAGDFFTELGDKAQQGFASIKDKAIE
                     NFSGLGEWFKETWSKIGDAIGSGLKSGLNFFTSTVPEWFAGIGDKIKEECGKIGGKIS
                     SFFNETMPAIINNIVEWFKQIPYNIGFIIGSIAGFFVDLGANLYAWATETLPEIINSI
                     VEWFASLPEKISEFFSIILQNIQIWGENFKVSVSEFFSTIWENITQFFTELPERISEW
                     FNTVVETVSGWGENLKQLATDIFNQFVDNVLIPISELPGKLWEKFTDIMGKVSEWGSN
                     LIQKALETGSKFLENIMKFFSELPGKIWEKLTNIMQKVMEWEVNLSKKALETAKNFFN
                     NIYNTVSQLPGKFKEWLDSIINNVISWGTNLVKQAGEAGKNMANAVKDALKDLPSKIM
                     SIGKDVVRGLWEGITGMGGWLKDQVLDFAGNVIDGFRDGFGVHSPSIIMRDLIGRNLV
                     KGVGVGIDVETPGLKEKIEKNISELTGKLKATVNFETSKMQANIVASTDFKAGKETAI
                     MGNNKTDEANENQAGIKLNIENFVNNRQQDIENLFDEILFLAKRKGVL"
     gene            36060..36794
                     /locus_tag="MIW95_000753"
     CDS             36060..36794
                     /locus_tag="MIW95_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HBY3150101.1"
                     /translation="MFFAYDGRDSREFGLKIYNINDLSAPQIEVERVSVPGKDGDLLL
                     KKGFGNFTLTIECDIDARQSNIEEVATEIKKWLQGDISYKKLFLSNSDFYYLASCNNK
                     LDITRNFKNFASCLLTFDCYPFRYTEEEIISLNVLNLKSATITNFYRESKPTLYIEGT
                     GDISIKINTQNIVLRGVAENGILSDLIIDSEQMNVYRINKENNIIVNENNKLFSDFPI
                     LEEGENLISWEGDIKSIKINPRWNIL"
     gene            36883..38922
                     /locus_tag="MIW95_000754"
     CDS             36883..38922
                     /locus_tag="MIW95_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBY3150102.1"
                     /translation="MGLGVLTNVINDHVREEESGVFELEFTIYEDSFLFKEIKTDRLV
                     KADASPDFKNQLFRIYYISKNLGGYINVKAQHIKYDLLNNFIESLKLNDITCEDALER
                     VFRACEEQNRFRGHSDIKARNSINISMQSPYNAICEGENSLIQIFEPSAKLFFDNYDV
                     FLNYQRGESKNVLLAYKKNITGLEAEYDTQDIVTKIYPYATYDDELITLSEKYITSTR
                     LNEYATQKIVAIDFSSDEVSVEEELREKCKDYFKYNQVDLPKVLYKVNFIDLSTTINY
                     KDYKMLETVNLGDEVIIRDFNLNINATARVVKTDYSPIQQKYYSIEVGDLINHLDFLN
                     NKFNNIENKIETVKKAIDNVKVDDSEFPDTLPDIPILTAEGLFAFINLTWTFTNKSYY
                     KYEVYASQIKDFAPDTINFTNRIFVGQASAYVHQAEPFQTWYFRVRAVNSHANVTEFS
                     NQVEAQTTKIDDGTTWIEKGAIADALIGELKLDRGWFGQLKGVYINAREMVVVNDNGV
                     KTMEIDSFGDIHFNPNTFKIAFNNISPYVLIDDEGLRLISGKGFTKMTDSGLYTKFYT
                     DSDVTESWYLQESGYTYFDYKDSKIIIDLPKKFAGKNFKATCAIRSTSTSGSCLSYFS
                     TSATVKKSDTKPQLELSASVRGVSYEMSGSQTIGYWLGMNFFSEGFITVQYYVYA"
     gene            38948..39190
                     /locus_tag="MIW95_000755"
     CDS             38948..39190
                     /locus_tag="MIW95_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150103.1"
                     /translation="MVTIFYSKRTGEIYNCIKSEVEQDYTCFADREEDYRQILDKIVV
                     EDIPEILNPRDYKIENGKFVLKEKEIIKEFTPKMVE"
     gene            39205..>39743
                     /locus_tag="MIW95_000756"
     CDS             39205..>39743
                     /locus_tag="MIW95_000756"
                     /inference="COORDINATES: protein motif:HMM:NF022117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBY3150104.1"
                     /translation="MRDRIYTVDINTKSYQVAKYKQYDNAIEFKINLLENNIEKDLTG
                     YTAIANFQRPDGKIVYQSCTIENSIVTTIIENNITAVAGDVIVEFTFYKDDLVVTTFS
                     LKINIEKSIDKNSITEEPKWDYISVTINQVKEVVEGIEEIKETEEARKEAEIKRVEEF
                     NSIKETFDSKVTEVTDAKN"
BASE COUNT        15710 a         4738 c         7556 g        11739 t
ORIGIN      
        1 aatacgacta aaacaacatt aatagacgaa gttaacacag tcaaagcgga agttacaaca
       61 gctaaaaata atatgattag cgaagtaact acagctaaag aaactatgca gacagaagtt
      121 acagacgcta ttaatgcaat ccccacaaaa gaagagttaa aaggggtagg aatagaaata
      181 aaaggaagtt tagataatat atcagcactt ccagtcaatc caactttaag tgatgcttac
      241 tttgtaaaaa gcgcaacaat agaaaaccaa attgatttat acgtttggga caacactaat
      301 tgggtcaaag ttcctgacat aaaaataaag ggtgaaaatg gagatggctt agaatttaat
      361 tgggatggca cacggctagg tgtaaggata gaaggacagg agaattatac ttatacagat
      421 ttaaaaggtc aaaaaggcga taaaggcgat agtattgaat ttaattggga tgacacacga
      481 ttaggtataa agatagaagg tcaagaaaat tatagttata cagaattgaa aggggagcag
      541 ggttatactc caacgatagg agaaaatgga aactggtgga ttaataatat agatacccaa
      601 aaaccagcta gaggggcatc tttgaggatt ctagggaaat tggattctgt agataagtta
      661 ccactagacc ctgctattgg cgattgttgg attgtaggaa gaaatattta tatatatcaa
      721 actgaatgga aggatttagg ttctctagcg ggtgtggatg gcaagaactt agaatttaat
      781 tgggttggaa cacagttagg cgttagacag caatacgaat tagattataa gtatatagac
      841 ctcaaaggag ataatataga atttaattgg gatggtacac gactaggtgt aaggatagaa
      901 ggacaggaga attatactta tacagatttg aaaggtcaaa aaggtgatag cattgaattt
      961 agttggaatg gtacggagct aggtgttaga atagaaggtc aagaggacta tagctataca
     1021 aatctaaaag gagcgacagg aaataaatta gaatttaatt ggaacggaag tcagctaggt
     1081 attagagaag aaggacaaac ggaatatata tatacagagc ttagagggga acaaggttat
     1141 actccagcaa tcggcgaaaa cggcaactgg tttataaatg gagaggatac cggaaaagcg
     1201 tcgaaaggaa aagttacatg gaatgagtta ctagaaaaac caaaagaatt ggattatgct
     1261 aagaaagaga ctatattcaa ttcagatggt tctataacag atatattaga ctcaactaac
     1321 aagactataa ctaagtttaa tgcggatgga actatagtag atgaaaaata tgtgaatgat
     1381 atacttgtga gtaaagtaaa aactactttt acaggtacta aaatagaaga aataaaagaa
     1441 aaaatagagg tgagttgatg agttgggcgg agatatacaa agtaaatagt gacttgcaag
     1501 gcgagccact taatttttta agttatttgc aagatataaa actaaatgga ttagatagtt
     1561 atgtgctgtt tattggaaat gctaggatat gggaagaatt atatttaaat agtttatatt
     1621 tattttctga taaaggaata agagaaacag tttacacagc tttttcagaa actgatattg
     1681 ataatttatt taataaaagc actaagctag gggaacaatt aaatgccttt tatagaacag
     1741 atatatttag tttaggaaat gctgataatg tagtaaaagg aatgactata gagcattata
     1801 attcattaga ggaaaagttt aaagcagggt atgatagata tgttacaagg gaacaagaaa
     1861 agtcaactat aggagcatgg tttaattcta cttttagctt agataatact gatttagaaa
     1921 atcttacaac tatagaagaa atattagcaa atgtggaagc tactaatgca atacttaata
     1981 atagtaatgc aatagtagct ttaactatgt gcaaaacaag tatggacgca gttgtagcaa
     2041 gttcaaacgc aatggatttg ctaggacaat acattttaaa ggtcacaaca gagcaacaag
     2101 ttataagagc gataattaaa aatgaagtta ttagagatgc tataataaat agcaatgaag
     2161 ctatgacgca aatttcaagc aatgaaaatt ctgtgatgga aatatttgat gacttagaag
     2221 ctacaaaagt attagtgcaa aaccaaaata gcataaataa aatattaact aataatgtta
     2281 ctgttgaaaa aataatccct aatttattag aaatgaagag caatttgcaa atttctttaa
     2341 attatataaa aacaataaaa tctaatattg tatctagcaa aggtcaaatt ttagcaataa
     2401 cttataacga aaaaatattc ccgcttttga aaaatataga aaaaaatcat gataatatgg
     2461 aaaatatacg aaatctatta caacaagatg tcgaggaaaa aataaaaatt gcggatgcaa
     2521 ttttaggaag ttcaatcgca atgacagctt ttgcaaataa ttctattata gtaaataaag
     2581 ttggtgacag agcgggaatt atagaaaata ttctcagtca aacagcgtca ttaaatgctt
     2641 ttatggaaag tacaacagct ataaatattt tagtaaataa aactactgct tttactaaga
     2701 tagcaaataa ctctaatgct atgtctgtaa tagcaaataa ctctaatact atgtctgtaa
     2761 tagcaaatag ctctaatgct atgtctgtaa tagcaaatag ctctaatgct atggcttcga
     2821 ttgtaaatag tcctacggct ttagatgtag tacttgcacg ttcagtagct atgagagaaa
     2881 tagcaaaaat tcaaagtgca gtaaacaaaa taacaggaac agctgttgcg ttaaataaag
     2941 tggttaaaaa tagtgcggcg aagagtgctt tagctaccca aaatgctaag ttgcaagcaa
     3001 ataaaagtgc tatacacact atactaaaaa ataattcaaa ttatttcaga gaaattatag
     3061 tatcacaaaa atcggattct tcaccgacac aagagattag tagcacttat ataggtgtaa
     3121 cttattgtta tggctataat ggaggttctg gatatggaat tgtataccat ggacatgaca
     3181 caaatataga agcgggtagg gcgaacggct cgcaagatga aaataaaaaa ttcataacgc
     3241 taggtggaac taaattcaca gaaagcggag atggatactt tatgtattca atatatcaag
     3301 ctatttaaca agctatttaa tatttacaag tcatctaaaa gattagatta acagtctaat
     3361 ctttttatat ttaaaataaa aaatcaaaat taggaggaaa ttttatggaa gaattaataa
     3421 cgcaactaag tgggctagga gctgtaggga tattatgcgc tttgctattt aaaaacacca
     3481 tgcaagagaa aaaagaagat agggacatgt ataaaaagac agtagaaaat ttcatagaac
     3541 tatctacaca gcaacaagag ataaataaga atatacttgt tgagatggga gcaatgaaaa
     3601 cagacgtaga agaaatcaaa gaagatgtaa cagacataaa agacatgtta cagaaagaag
     3661 gtctttaaat gagagtagca gtaacagcag ggcatacact agcaggcaaa ggaacgggag
     3721 cagtcggtta tataaatgaa ggaaaagaaa atagaatatt aatggattta gttgtcgagt
     3781 ggcttaaaaa aggtggcgct actgtttgca gtggtaaggt agataagtct aataactact
     3841 tagcagagca atgtcaaata gccaataaac aagatgtaga tgtagctgta caaatacatt
     3901 ttaatgccaa caaaacaacc ttaaatccaa taggtacaga gacaatatac aaaactaata
     3961 atggcaaagt gtatgcagat agggtaaata ctaaattagc aactgtattt aaaaacagag
     4021 gtgcaaaatc agatgtcaga gggctttact ggcttagtca tacaaaagct ccagcgatat
     4081 taatagaagt gtgcttcgta gatagtaaag cagatacaga ctattatatt agacataaag
     4141 acatagtggc taagttaata gcagaaggca ttttaaataa aaatatagat aataaagaaa
     4201 atagtgagga taagaaaatg tataaacaca cagtcgttta tgatggagaa gttgacaaaa
     4261 tccttgcaac tgtagttggt tggggttata atgatggtaa aatattaata tgtgatataa
     4321 aagattatgt acctggccag acacaaaatc tttatgttgt agggggaggg gcatgtaata
     4381 agatagggtc tataactaaa gagaagtata ctgcaataaa aggaaatgat aggtttgaaa
     4441 cactttacaa agcattagat tttattgata gatagattaa gaagtagcaa ctaatttatt
     4501 atatttgcct gaattgtgtg ttagctaacg cacaatttaa attaaaagag aaggtagcaa
     4561 ctaatttgct agttgctacc ttctcttttt tattttattt tttatataaa tttaattatt
     4621 atctttaatt ttaccaatta attcgtctat tatttcaaaa gccaattcgt ttgaaataga
     4681 tatatcatga ttattagagc aatattcttt tacataaact tcgtatgtat cagatttaac
     4741 attgcttaaa taaaaatcta cttccgtttc ataatcttca atgtagtcct catgacatcc
     4801 ttcattattt tctataccat ataattcctc gacaatagtt tcttttagtt gttcagtaat
     4861 atttttcata aaaacatccc cttttcattt ttttaaaatt agtaaataaa tatcttactt
     4921 gctgtattct ctttcaagag aatctacatt aaaaacccat gactttccga aaagtctgca
     4981 gtcaacaccc tcgacaaatt taccattctt aatattctgc ttcaaggtac tttcagcctt
     5041 attatacttt ttagatgcgt cgcttaagga aataaaatta ctaaaattat ttagttgctc
     5101 tatatatcca ttataaactc ccacgacaaa attaactata acctcgtaat tatctaagtt
     5161 ttcaacgatt tcggtaggca taggtatgtc caacttcata aaagccttta aaattatttc
     5221 aaatgctttg tttctatctt gatatctgta aattgcatca tttaattcta aaattttatt
     5281 tctacttaat acattctcac ttccgatttt aaaacctaaa ttgtatagct ctttatttaa
     5341 ttttgcattc ataaaattca ctccttgttt ttgtttatat aattattata gtcgatacaa
     5401 actaaaaaag caataaaaat agtcttaaaa aatatttata gtctttttag actattttta
     5461 ttgacaaaat agtctaaaaa gactataata taattaaaga taaacaaaag ggggatttaa
     5521 aaaaatgaaa aagataacta aaaaagaaat aatagaattt gtaagagatg tagtagaaga
     5581 atatcgtgat tggaaattag aatcatgtgg cttctatata aaagataatg aattaaattc
     5641 ttttataagc tttgaaggaa aaggaattga cataaatgta tataaagaaa actacgaaga
     5701 aataatttac atagaagatt atataaaaga ctacaagtgt aaggaatata acttaaaaga
     5761 aatagattct atcatatatg aagatgtaaa tgaaatgatt agtaactata atgaaaaata
     5821 aagaaatgaa aataaaataa aaaaattact ggaggaaatt aaaatgaata aacaaaaagc
     5881 tagaagattt ttaaatgtta tagatatgaa tatagataaa atagaagaag aagctataaa
     5941 agcttttaaa gaaagctgtt taattaaaga ggaaaatgat ataaaaattt ttattgattt
     6001 tcaaggaaaa gttgaagcga tagcagtttc gacttgggct aaacttttag atgatgacaa
     6061 agaaattaat attttcacat taactcaaac accaactcat ttagacgata tgtttggaga
     6121 actttgctac gtaaatgact acgaagagtt tgaaaattgg tgtgaaaatg aagacgagag
     6181 tttgaattgg gatagttata aaaaattcaa taaagaaaat ttcgaagaaa tcactgaaag
     6241 aaatatagac gatagttcat cagttttttt agaaaaatta cgagaaggca ttgaaagttg
     6301 taaacaagaa ttacaaaata tagttgaaaa ttaagtataa gagtattatt attaatgtat
     6361 atagtatcgt ttttttagat taaccatgag ggatttaaaa tattttttaa tttatttaga
     6421 aatgatattg taataagaat ttttagatta actatttagc attaaaaaac agtgtattta
     6481 ataaatacac tgtttttaat gctaaatagt tagttactat gataacttaa tccttcgatt
     6541 ataaatccta cgttcattgt catttctgct tcgtcgcttt gcaatattcc ttccgtaaat
     6601 tcgctgccgc ttggcaatga tacttttttt cttgcaacta cttctatgat tgtttgaaat
     6661 agtttaattt tcatattctt atcaaatcca gcgtatcgca aaaaatttac aacttcttgt
     6721 gaaaacttat ttaattcaga tttagtcatt ttttcagata actcttttcc aatttgcaaa
     6781 taaacattca tcttttccaa ccccttttta gattatagtt aaaacatatt atttaaacaa
     6841 tatcgatata ttgtaataat aatattaaaa tattacttaa atattatata tacaatattt
     6901 aagtaatatc taaacaatat aaatataata ttatttactt tttcttagtc ttatttcttc
     6961 tatttctctc attgtatata tttaaattct cttcttttat agtgacatca gataaaattt
     7021 gagttagtat atcgcttaat aacttgctaa cgtcgctgtt catatcaata gaaaaagatt
     7081 ttaatttatt tacaagctct tcatcaagcg ttgcactcat agtcaccttt ttacttttct
     7141 tattaactag attatatatg tttaatgtct tattgtctgt agttgtagaa tcattttgta
     7201 cgattgtccc gtttttcaaa actggttttt ctttatcaac taaaaggtct ctatcgaaac
     7261 ttggcatcta taacactcct ttcactttta gttcttctaa catattagta aactcttgcc
     7321 tactgaaatg gtctttagtt atttttgtat aatctgttag agatagtttt ttcaatagtg
     7381 ctttctctat aaactcattt tttctaatat atgtatctaa cataatatct ctcatatcgt
     7441 taaacccttc cagataagaa tcgaaagtat cactaatttg agttttcttt ttctcaaatc
     7501 caactaaaat agtacttttt atattatcct ccttatcgaa ataggttcta tcaacatccc
     7561 agagctgttt aaataagtct gcccctctaa ggctcgaaat gtttttatct tgaattagaa
     7621 taattacact atcagccaaa aaaagcacgt ttttagatgt taaatcgtag cgcggagata
     7681 aatcacagat aatataatca tactcactca aaatattaaa gttttccata taccaacgag
     7741 ctaaaaattt ttctctagct ggtagaggtg atagctcctg ttcaaatcta gccatttgta
     7801 tatcagaagg aattaagtct acattagtgt aatttccatt aggcgacttg actattacat
     7861 cattagcact aatccctttc aaaatatcta atgtagtatt atcattatga tttatttcat
     7921 aaacatactg tgttagattt gcttgtacat ctaagtccca taagagaatt tttttatctt
     7981 tatcttttgc aagttcatac gctgtcatta tagaagttgt tgtcttatat attccccctt
     8041 ttatgttaaa atacgtaatt aattttgtat ctgccatgtt tccaccccat tattatattt
     8101 ataatattat aataacattg tagtattgtt tttataatat cgcaacaata tatttatatt
     8161 attaaataca atattttaac aatatgaaca caatatttat atattatttt ttataatatt
     8221 aatacattat tttaataaca ctaaaatatt atatatataa tattaagata atatttttac
     8281 aatattacag tattattttt atattatagt aaaatttgaa aaagaaggct aagaaaaaag
     8341 caaccttctt ttgtagaaaa agttattcat tcttagcctt tttaataatt atttcgtcgc
     8401 cttttaaagt tacttctacg ctcctgtcgc tttcagttac acctaattct cgtaagaatc
     8461 taataggtag attaagccta gtactcgtag agccacaacc acttttgtta aaattaatat
     8521 tcaaaatcct tgtttctttc aaaactacca ctcgcctttt ttgcgttgcc aacgaaaaaa
     8581 tattaataat aaaaaactca taattaacca aactacattg cttaagttaa aatcttttac
     8641 tagaagaatt aagtctaaaa ctattgtaaa aataattaca gcaacgcata ttttatatac
     8701 ttttttactc atacatttca agaaaatgtg ttataattta agaaagaagt cactgagaag
     8761 gaagtgcaat tccttcccag cttaaaactt acaagtcttt tagtaatttg ataagtctat
     8821 ctatcaagct tactaaaata ctcagaatag agagaatgat tttaactttg ctgagtcttt
     8881 tctctctatt tttttgtctt ctttctagtc tactcatttc cttgttgtca cctcctttct
     8941 ataatataat tatagcataa cgtgacgata aaaacaatag ttttgcccta cttttttaga
     9001 aaattttggt atattgaaat gatataaatt cgtatctttt ttaagaaatt gtgatataat
     9061 aaaagcaaga gaactatatt ctagtcgtaa gagtgtagtt ttcgtctaaa gtaaaaaaat
     9121 atccagactt ttgaatttga tttttaaatc aaattcaaaa acgctctttc gtgcaaaaga
     9181 gcgtttttta atttcaaaca ctttaataat tgaaaaataa acaaatatga aatgatataa
     9241 aattgaatca ttttatctaa aatatgatat aataaaaaca aggaaataat gatttacttt
     9301 atacaagagc agttatttcc aattcagatt aaaaattaat tactttttta aactactctt
     9361 attggcgttt gagtagtttt ttattttctc atacataaaa gctgatacaa caccagctag
     9421 tacacttaat aaaaaatcta ttaccatatc actccacctc cttccgttat ggaatttggc
     9481 gtttaacatg gaaataacta ctcttacact tttcaatcat tttttccttg caagattatt
     9541 ataacatata tttacatata aaaaagactt agtaaatatg ctaagtcttt taaaatacag
     9601 ttttttagga aatatgtttt taaaaaataa tttatttttg aaaacagtat atttttttat
     9661 aatttaaatg tgctataatt ataatcataa taagcagaaa aaactttttt taattggttt
     9721 aaaaaaatct acaaaaaaat aaaatgagga atactcactg ccgtcaaaca atagtattcc
     9781 tcgcacacga ggttagacct cttttgaagg ataatatatc cttgtttttc tatacttata
     9841 tttatattat aagataaaat atagaaaaac acaaggtttt tagggtaatt ttaatagtaa
     9901 aattattaca aaaaatcgga aagagtttta acctccaggt gaaaaaatgg agggataaaa
     9961 gtgaaaaata ctaataaaaa taatatagat aaattatttc tagaagaatg taacgctgaa
    10021 agagcagagc atgaaaagga aaattattct gaatttaata gacatgaaat atatgaaata
    10081 aaaaaatata tagatgaaaa accgcttgga attagaacag ctatagccaa tgcagatgat
    10141 agaggaaaaa ccttatttat tgcaccaaca ggagcaggaa aaagttacag ctttataaat
    10201 acattaaaaa aattaaaaac taaagcatta tttatacttc ctaacgcttc taatgtagag
    10261 caagcaatgc acgaatatag cgtagctggt gcatatgata agatacctgc taaacaagcc
    10321 ctaaagggca ataatctagt agtgatgact tgggataaaa ctgaacaact catagatgta
    10381 gacttaagcg agtatataat cgtagtcgat gaaatacatc aaacatacac agacgcttat
    10441 cgagcgaaag caataaaaaa tctaaataat ataatgacaa aatgcaaagg aagaatagat
    10501 ataacagcaa ctccaacaaa attggaattt gaaatatatg attatataat agagtatact
    10561 caaacccaaa aaacaaaata caacgttaaa ttatacaatg actttgacaa taaaaacttt
    10621 acagaaataa ttaatataat aaacaaatca aaaaatagtg caatgctaat gaatgatata
    10681 tcaactcttg aatttataag agattcagtc gacaaaaacg ctgatgttgt atatgcagat
    10741 gggaaagaag agaatgaatt atacaataga atagttaaaa attcagatat gaaaggctat
    10801 gaaacgcttt taaatacaac aactatttta gctggtgtta acataaaaaa taaaaatatt
    10861 acagatataa ttatagtgaa tataaaagac gttggagcta taaagcaata tgttgcaagg
    10921 tttagaaact tgaaaaaagc aaatatacac atatttaata aatacgatga agagtgcaat
    10981 atttataaaa tcgagtggct agttaataaa aacatagaaa aagcaactat attaaaagag
    11041 gcttataaca aagtatctaa acacatgtta caatttgaaa ctgtcgggat aaatgcaaca
    11101 ccaattagaa tggattctaa tgtttactat tgcacaaaag ataattgcta caaagtagat
    11161 aagttatata taaaaagtca agtttacagc aattattata atacaagaac aattcaaagc
    11221 tttaaagtac tattagaaga atacttcgag aacattgaaa tagctgatat gaaagaaaat
    11281 atcacaaatg agaaagagtt aaaagaatat aaaaaagttg caaaagagat taaagacgca
    11341 acaagagaaa ttttaaaaga acataaagaa attctagtag gctacagaca gattaaaagc
    11401 gattctaaga gctttaaact aatgcagtac cataatgata tgaaactgag ttctgagggg
    11461 tgtttgaagg catacaagga gtatgacata cataatttag taaagaaaag taaaagtaat
    11521 agcatgctag aactatattc taactacgtg ctagataata aatttagttt agaactagct
    11581 tggaaattag cgaacacagc aaatagaaag cgtggggcaa tatttaataa aataaatact
    11641 ttaatatata gagaattaaa agaagaatat ccacaatttt taaatgatga acttatagaa
    11701 gtgactaagt ttaactatat agacaaatta tttggcgttg ggacatctta tacaaaagtg
    11761 catttacaag agttaacgaa cgatttaaga cttatcttag gggaagtatg ggatttaaac
    11821 actaaaaaat tagagattat actaaatgga attttcgtaa tagaaaataa aaactataga
    11881 agtggttcag tattagaaca tatctttttt tataaaaata ttaatcctaa tactgaacca
    11941 gggacaagaa aaagaattag aattaataat ataaaaagat acataaccat agacgacata
    12001 aaacaagaat tagaattaga taaaaaagat aatagcttag agcatgctat agaatataca
    12061 aaagatagaa tgttaaattc cttagatgaa gaggaaaaac tattgctatt aaaaggattg
    12121 atgtaagaaa taacaactga gaaaataggt tgcatattga acgaaatttt tatgatagaa
    12181 aagaaacaag ttcgaaagtg ctcagtatta gaaagtatct tttttataaa aatattaatc
    12241 ctaatactga gacagaggaa aaaagactta aaattgaaaa agaaaaagaa aaaacagagc
    12301 aactagaaat ttgcatttaa acaaaaagtc aacggagtag gaatttatat atatatataa
    12361 attcctactc cgttgacttt ttgatatgga gctatagggt ttaaatattt gaccctacaa
    12421 ttttttaatg tgagaaaatt caaaataaat tgaattggaa ttattttcca gtagcaaaat
    12481 ttgctaaaag gtactattat tgtagtataa atgtttaatt acgacaaaaa atcgtacatg
    12541 tgttcttgta atatcgaaca aaagggggta taattaaaat tgtaagttat tttttacaaa
    12601 ttttaagaat gtattattct atctgcatat agggggatga tttggttgag agaggacgag
    12661 tttagggaaa aaatagtagc aattataatg caaatcaata atattaatgt tttaaaattt
    12721 acctatacat acttaataaa agcaaaaaac aagtgctaaa tagcgcttgt ttttttattt
    12781 ctttaataaa ccatcaatta atttatttat taaatcaaga tattcctcat caagttcaca
    12841 aagttttatt attatttcct ttattggaat attatccgaa atagttatct ctgctagtac
    12901 ttctgccagt aatgaatctt tttcatttat cgaatatttt tcgccctctc caaatcttag
    12961 ccaattttca ttcacattaa attctctaca gatgtctttt attgaccggt cggttgcgtc
    13021 ccttattccc ttttcgtaac aacttatttg gcttcttgat aattttactt tacttccaaa
    13081 atctttttga gataaaccag catcttctct tatttctttt attctatctt ttatattttc
    13141 ttcttggatt tgttcgcaca agttttacca ctccctcttt gaattttgcc ttaaatatag
    13201 aataaagcat tttagtttac aatgcaacac ttaattgaaa aataatagtt gcattgcgga
    13261 acaagatggt gtattattaa attagaaatt ctactttgca acacaattat gaaaggaagg
    13321 tttttcatga gggcagaaaa aattaaaact atagttagga aaataaattt cctggatgaa
    13381 cttggtcttg aaattgtaaa taatataggc gatttatgca ttaaagacca gaaaaataaa
    13441 aagaaaaata attcttatga cttagaaaat atcgtcagta tagataaaat ggactaatct
    13501 tgcaaagcaa cattaatgcg tactttgacg gctacgctaa accaattaaa aaagtaaaaa
    13561 agtaaataag gaggcttaaa aatgataaaa gttattttat gcaatactat cgacgagtac
    13621 atgaagtatg aggacgtaat aggagcaaaa gacagctatt taatggattt tgctgtaagt
    13681 gatgaatgtt cagaagagga tgctatgaaa gccttggaca ctctagggaa tagtaaagtg
    13741 aggattatgt cagaagtagg gaaaatggct ttcaattttg gcgacctaat cgcaaaaggt
    13801 atggaacaag gcaaaaaaga ggctccaaaa ctaaaaagga ggatatgttg tgataaatgt
    13861 ggggcaagcg tagacagttc ggaggaatta acttcaatgc cagttttaga aaattataac
    13921 aacaatcgtt ccgtttctga ccaactagga agtatagacg tatgcccaga ttgtttgcta
    13981 gaaataacag acttattgta attccctgtt ttatctgaaa agaaacaaaa agtaacagcg
    14041 taagccgtca ataaaaaaag aagggaggat ttaaaaaatg attaatttag aaacattgtg
    14101 caatggagag acaaaagaaa agattgaaaa agggttcatg gagatattta aaaacataca
    14161 agaccctaat acgccagcaa catctacaag gtctttgact gtaaaggtta cactaaaacc
    14221 aggcaaaaat cgttctcatg ttaatacaca aatacaagtt attccaaagc tcgcaagtgt
    14281 cttacctagt gaaacggaca ttatagttga aaaggatttt agaactggcg aagtagacgc
    14341 aaacgaatat ggaaatcagt tgccggggca agtaaaatta ggcgacttgg agagtaaaga
    14401 aacaagtgta acagaagaaa cagaagtaaa agaaagtgaa gaagataata ttcgtaaatt
    14461 taagagttta aaagacttat aaaaactaaa agggagtgtt taatatgtta acagatttat
    14521 ttaacagagt aatggaagtt ggagcaagaa aaatagaaac aaaagaagtt aatggggtta
    14581 catatacaac agaccattta attccagtac attcaaaacc agttgaaata attggtttaa
    14641 atacattgga cggtttaata gggtatataa aatcagagtt tgatagtgat gctaaaagtg
    14701 atagacttat agttcatgtt gaaagcccta agagtgttgc attgtactct gaattaatgg
    14761 caagtgggaa aaggaatatt tatgttgata gtaaagcatt attacctcat atcaattttg
    14821 gttgttcgct tgatacagaa gaatttaata ttatgttaca atcctctttt gtagatacag
    14881 aggataagaa tttactttta aaagtgagtg gaaatgtaaa agaaactagc gtaaaaactg
    14941 tgcaggatga tggagtgtcg caagttgttg caatgagtac aggcgtggca agcgtcgagg
    15001 atgtaatcct gcctaacaga gttaagttaa aaccttacag aacttttgcg gaagtagacc
    15061 agcccgaaag tgaatttatc tttagagtaa aagagggcat aacatttaag ttaatcgaag
    15121 cagatggtgg agcatggaga cttgaagcaa taaaaaatat aaaagagtac ttagagaaga
    15181 aattagaagg tattgaaaat atagatataa tagcataaaa aattgaagag gctggactaa
    15241 ataaccagcc tcttttaaaa aggagtgttt attttgtata aggatataac aaaaatggga
    15301 gcaatcgaac gtggggaata tgttggtaat gttgctcttg aattaaggga agaaaatcct
    15361 catcttagta aaatagattt aattgagtta gctcttaaga aaataagaga ggaaagtgaa
    15421 caggaggctg ggcaatgaaa ataggtaaat acttgatcgt ttccaaggaa caaatggaca
    15481 gaaatattga atgcttaaaa gaagaaatca aaataaggga cgataaaata aggttgcatg
    15541 agtgtattat gagcaataat gaagaattga ttcaaatttt agaagaacag ttgaaagaat
    15601 ataaaagtga actaaaagag tataaaagcg aattagatgt atatagcttg tttatagatt
    15661 gtattttaaa taaagaaaca aggaaagcaa tagcaattta taacaggact aaaagtatta
    15721 gaattaaacg aaaatgtctt aattctatac ctgtaaaaaa gggttaagtg aaataaataa
    15781 tttccttgta ggaatagaga gggatgtttg atatatggat gaatttgtag agtgtaatcg
    15841 ttgtggaaga ataataaata cagaggaaga cggttgtgta aaatatgaaa aagaaaatct
    15901 aggaataatt gtaactctga atatgtgttt agattgttta gaagaattaa ttgaagatgc
    15961 gaaatttcta atataggtta tgacggaggt atgtaaatat gaaaatgata aatgttttta
    16021 aattattatc aatgtattgt tttgcctaga gtgtggaagt gatgaacttg gagaaggtga
    16081 aggaagttta atcgttgatg agtatacatt tcatagaaag tgtaaatgtg gatttgacat
    16141 gatagtagat gaaagggagg acaaaatata atgaattttt taggaatgat aattttaatt
    16201 atatttgcat ttttcattgg tcggatatat gaatatagag ttaatttaag agaatgtgag
    16261 aattgtaagg gagggtttga aaatgaataa tgttgtattg attggcagat taacaaaaga
    16321 ccccgaatta aaatatattc caggctcggg gacagctgta tcgacttttt ctattgcggt
    16381 agacagagac tatattaaaa aagatgggac taaagaaact gattttatac ctgttgagat
    16441 aatgggaaaa ttagcggaag tatgtgctaa taatttaaat aaaggcagat tagtagcggt
    16501 tcaagggtct ataagagttg aacactatga aaaagacgac gagaaaagaa cttatacaaa
    16561 agttcacgct aacaggatga aatttttaga ttacaaaaaa gaagataatg gaaaagaatg
    16621 taagttcgag ccgggaggct tagacccaca aggcttccag gctatagacg acgacgatat
    16681 acctttttaa gtaggtgatt aaatgattaa aaaagtagaa aaaattttta gtggttcagt
    16741 taatccaaat tttatggaag atgacccaac ttttataaat tatttagcgt tatattgttg
    16801 cataaaacca gttaaagtcc gtggaaataa aaaggttttg gctagttcca ctaaggcatg
    16861 cagtgcattg aaaattagag aacatcagaa aaaagaacgt aaaaaggtaa gacttataaa
    16921 tgcaaaaaca ggtaaaataa aagaaatggc tatagatgaa gcagaaaatt ttttgaatgt
    16981 taaaaacttg tatcctgtta tttggcgagg gagaccaact cgaactggtt ggtatgtgaa
    17041 ggatgttgaa gatgaaggag attaaatata acgttgtgta cttcaattta aagactttga
    17101 aatttagtga aaaaagtttt aacactttaa aagaggctag agcatttaaa aaagaaaaag
    17161 agaaaaaata cgaaaatgtt gagattatta aaaagacaat tatagaaaaa ttgataatat
    17221 aaagaaggtg cttatatagt gcgagtaagg cgaatttgtt catggtgtgg taagctattt
    17281 tatgcagaat ttaaatcgaa gcaaaatttt tgttgcagaa agtgcaaaac taaatataaa
    17341 aagagaagca aggagcagga aagcagggag attgattatt ttaaggaaga attgaaaaaa
    17401 agttaaaaaa catccttcat tgaatagata ttgcatgagc gagttaggca ttagggaagt
    17461 tttaaatatg aattttgaag gtaattaatg gaggttttta tggataagga aagaataaaa
    17521 aaaactggtc gctatttgaa cgatttggga gataggaagc gaagaatcaa tatattaaaa
    17581 aaagagattg ccattttaaa agaaagtgaa aaatatagcg agattaattt taatgaatta
    17641 ggctttaaaa taaaaacaag cccgaagggt ctggatgata tgattattaa ttcagaacaa
    17701 caaattcttt taaaagaatc tgaaattgaa tatatagaaa aaaggttaaa aattgtttac
    17761 tcatatatgc aagaattaga aagtgacgaa agagaaatta taaggttaag atatttttat
    17821 gatataaata ataaaatgac aatgactaaa atagcgagtc aggttaattg ttgcagatgg
    17881 aatgtttata gaaaacttga taatgctttg tggaaattat ctggaatgct ttcaattttt
    17941 taaaaaacta acaagtggtt gacacaaaaa acacatgtta cacaccaaat tgtttttttg
    18001 aaggtatata attgtattat aggaaaaaaa ggataaaata tattcccctt ataaaaggtc
    18061 tagcaaataa ttgttagacc ttttttattt ggaaaaatgg aggaatagaa gtgaaagaga
    18121 aaattaagcg ctgggtagat gctagcgaag ctattgaagc atatgaaaag aacgttgaaa
    18181 aaataataga tgataaaagg aggattgaaa gggaagcgtt gagagtagac ccaattaaaa
    18241 gtcaaaaaga catagataag gttaaaagat atctatataa aaaagataag cggtattact
    18301 tgatatttgt cttgggcata aacacagggc ttagaataag tgatattgtt aaattgaaag
    18361 tcaatcaagt atataagaga acacatgtgt atcttaaaga aaaaaagact agcaagtcta
    18421 acaacataaa aatcaatgaa tcagcaagag gggctataaa tgaatattgt tgtaatttaa
    18481 gtcaagatga ttatttattt ataagtcaaa aaggtgagta tctaaaagaa aaaacagttt
    18541 acaaagtgct taagaaagca ttcaaaaaat gcagattaag aggaaatttt ggtactcata
    18601 cacttaggaa gacatatgct tggcacatga ataagacgga aggtttagaa gtggtacaat
    18661 gggcgctaaa ccatgtaaat caaagcgaca cattaaagta tttaggaatg aagcaagagg
    18721 ctttagacag agcagttgat aggttaaact tgtagcttat ttttaatcta aaaagcgtcg
    18781 ttttcggatg ttcgtagcat tttaaattag aaaataaaaa gtaatggctg aaagtaagtg
    18841 ttttcaataa acaagatagt atttttaaaa tgcgtaagta taggtaaaag gtgcattttt
    18901 ttatcatgtc taagatattg atataactaa gccagaagct tgaagggtct tgttagggaa
    18961 aaagagcatt aaatttgaaa atatgaaaac aaggatggag ttgttgcaaa ttattccaaa
    19021 accccaaaaa atcaactcga aaaacagggt ctacgctagt tattgcaatg cttacaagtt
    19081 tttctttttc tgaattatta attttctgct aaaagtgttc aattaaagac ctgcttttgt
    19141 gaacaataga tatttcaaaa agataaaaaa tagaaactag atatttcaat gaaaaatatt
    19201 gtgtaattgt tcattttatt attgatgcct tttcgtgaac atgttataat ttaattataa
    19261 tattgtggaa gggtgttgat tatgaaagtc ttaggttatt gtagatgttc aacaaatgaa
    19321 gaaaagcagg acattgacag acaaataaga gatataaagc aattagcaaa agctcatggg
    19381 catgagttgt caaataaaaa tatttttaca gagtacgaaa gtggcgctaa aatagataga
    19441 attgagttaa atcgtatgtt ggatagctta ggggaaggtg acataattat atcgacggag
    19501 gtgtctcgta taagtcgtag cactaagcaa ttaataaata tacttgaatt agtaaaagaa
    19561 aagaaaaaca agttaatttt agggtggttt gttgtagatt gtacaaaagg cagtcttgac
    19621 gcaatgacag agggcatgct caaaatgatg ggtgtatttg cagagatgga aagagctatg
    19681 acaagtgata gggttaaaag cggtatggct aatgctaaag ctaaaggaaa aaatattggt
    19741 cgccctaaaa ctagcattga tagtattcca aatatatttt taaaacacta ttctaaatat
    19801 aaaaataaag aaataaatat aagtgaattt tcaagattgt gtaacatgag tcgtacaact
    19861 atatataagt atattaaatt gttagaggac tagactatct agttcttttt ttgttttgag
    19921 gtggtgataa atatagcaag acctagaaat cctaatcgag atagggcaag agaaatttac
    19981 aaagaaaaaa atggaaaaat agaactatta gagatttcgg atatattaaa tgaaaaactt
    20041 aagaatatac aaaaatggaa gtcggttgat aaatgggatg ctcatataaa aagagggggg
    20101 caaccaggca ataaaaatgc tttaggcaat aatgggggcg caccaatagg aaataaaaat
    20161 tcaagtgata actatttatg ttcatcattc aaaaatacaa tcccgagggg acttctaaaa
    20221 gtttggaata gtgtttataa tgatgaatta aacccgctgg actatttgtg ggctagtatt
    20281 acattgcagt attctaaaat tatatatgca ataaatataa ctcatgttaa gaataaaaaa
    20341 gaccatacga ttgatattac aaaagaggat ggcaaaaagt ataaggaata tcaaatacaa
    20401 cattcttggg ataaagaatt agcgtctatt aaggctatca caagtgcttt taatgtatta
    20461 actagaatga taaaagacta tgaagaactg cttcataaga attgggattt agcaacagaa
    20521 gaacaaaaaa gcagaataga aaatataaaa gcgagaacaa ataagttaac tggaaataat
    20581 ttagaaatag aggatataga agatatagag gcagaaatct atggcagtaa ctaagaagaa
    20641 aactatacct tttaaatttg gagataaaca caaggaatat attaaacgtt gcgctagtaa
    20701 cacatataat atagccgaag gtgctgtaag agccggtaag acggttgaca atgtgtttgc
    20761 ttttgcacat gaaataaaaa catctaagga taagatacat ctggcaacag ggtctacgag
    20821 tgcaaacgca aagttgaata ttggagacgc taatggcttt ggacttgaat atatttttcg
    20881 tggtcaatgt cattggggta aatttaaagg caatgaatgt ttatatataa aaggtatttc
    20941 aactaagcat aagcaaaaaa ttgttatttt cgctggaggt gctaaggctg atagttataa
    21001 gaaaataagg ggtaactcat atggcatgtg gatagcaaca gagataaatc tgcatcatga
    21061 taatactatc aaagaggctt ttaatagaat tatagcatca actaatcgta aaatattttg
    21121 ggatttaaac cccgacaacc ctggggcatc aatttataaa gattatattg acaactatat
    21181 ggaaaaggat aaagagggga ctttgctagg tggatataat tatcagcatt ttactataca
    21241 tgataatata accgtatcag aggaacgaaa gcaggcaatt atctcgcaat atgacataaa
    21301 tagtatttgg tacaagagag atatactggg tcaaagatgt gttgctgagg ggcttatata
    21361 tgaatacttt gctaataata aagagcagtt taagactgaa acagttgacc cacttatgga
    21421 tgttgttgtc ggtgtggatt ttgggggcaa caagtcttat catgcttttg tcgcaactgg
    21481 aattacttat aactataaaa aggtaatagc tcttgcaagt gagagaccta gcgcagatac
    21541 cagtccagac gagttaaata atttacttat aaactttata aaaaaagtga taaatatgca
    21601 tggtaaagta gattgtgttt actgcgatag tgctgaacag gtgttaataa aaggcattaa
    21661 aaatgcagta gaaaaagaaa atttaaatgt tagtgttaga aatgctttga agagtgctat
    21721 taacgataga ataagactta cagacttatt aattagtcaa ggtaggtttg agtatacaaa
    21781 gtatagtgaa actcttgtta atgctttgtg tagcgctgtt tgggatagca aagaaactaa
    21841 tgaggatata aggcttgatg atggttctag tgatatagat agtttagatg gttttgaata
    21901 tacgatagag agatatttaa aaaggtttat aaggggggac taaatgtttg atggattgaa
    21961 aaatgctata aggggggtga ttagcaagtt gtttaataag agttctatac aaaaagagtt
    22021 gaaagttgat atagctgtta gtgataagat gtcaagagct attgatttat ggatgtctat
    22081 gtataagaat aaatcgcctt ggacaaatga aataactaaa agtttgaatt tatctgctac
    22141 aatttcaagt gaagttgcac gccttgttac ccttgaactt gaaagtgaag tggtcgggaa
    22201 tgacttttta aatgaacaat atcaagatgt gttaaaagat ataagaaagt actgtgaata
    22261 tgcatgtggg gctggtggat tagtatttaa accatatgca agcgaaaaaa aaatagaggt
    22321 tgattatgtc caggcaacta attttttccc tgttgaatac aattctgcag gtgatattac
    22381 atcagctatt ttttcagaaa taaaagttaa aggtgatatt aaatacacta gattagaata
    22441 tcataaatgg acgcctaatt cttatactat ctctaattac gcatataaga gtaatgcagc
    22501 aaaattaatt ggttattcag atgatttagg gaagagaatt aaattaagtg atgttcccga
    22561 atgggcagag ctttcagaag agttagtatt agaaggaatt gaaagacctc tatttgcata
    22621 ttttaaaatt ccgcaagcta atagtttgga aaacacatca cctcttggca tttctgtttt
    22681 ttcaagagca gttgacctaa taaaagaggc tgataagcaa tattcaagaa ttttgtggga
    22741 gtatgaggct acagaggtgg cgattgatgc agattcaagc atgtttagaa gaaattcgag
    22801 cggagaatat gaagttccac atggcaagaa acgattgtac aggatgttag aaatggagga
    22861 tggagataac aaatggaatg tgttttcacc aaccataagg gattcgagcc ttttcgctgg
    22921 gcttaatcaa ttactaagaa aaatagagtt taattgtgga ttgtcatacg gtattatatc
    22981 agatgcacaa gagactgaaa aaacagcaac agaaataaaa actagcaaac aacgtttata
    23041 ctctactgtc aaggatatac aaaagtcttt agaggacgct ttggagggat taatttattc
    23101 tatagataag tggtctattt tggcaggatt tactcctagt ttgaagtatg aaacgacttt
    23161 taattgggac gatagcgtaa taatagacaa agattctgaa ctcttagcaa tgcaacaaga
    23221 tgtcgcatct gggttaatta gaccagaatt atatattgca aaaaaatatg gagtcacaga
    23281 ggaagaggct ttgaaaatga tgcccgacac tggggaatta gtagaggata atccttttga
    23341 caagggtggt atgtagatgt taacacctaa tgagttacaa gaaattccta agtattttgt
    23401 tagtttattt caagctttag aggactttat aatcgcagat attgcaagac gtatagcaaa
    23461 agttgggaac attacagata tggcagagtg gcagttaata agagcggaag agattggaat
    23521 tgctgataaa gttattaaaa gaaaaatttc cgaaatactt ggtatctctt ttgaggcagt
    23581 tgacaagtta tttgaagaaa gtgctataaa atctattgaa agtgatagcg ctttgtatga
    23641 acatgctaaa ttaacaccaa tacacttgaa tagttctgaa gaacttaaga actatgtttc
    23701 ttcagctaaa gagcagacaa ggagcaaact gaagaatatg acaggtacat tagggttttg
    23761 tactattaaa aaaggtaagg ttattagtaa gaaacttaca gacatatata tagagtcttt
    23821 agacttagca caatttcaag tcagtacagg agtcttggat tataagacag cgattaaggg
    23881 agctgttaaa aaattggctg atagtggact taggtttata gactatgaaa ctggctgggc
    23941 taatagaata gatgttgcaa ctagaagggc tgtgttgact ggcgttaacc agatgtcgca
    24001 gaatattaat aataaagtca ttaatgattt ggatactgat atagtagaag ttacagcgca
    24061 ttcgggggca aggtgcgagg gtgcaggcat taaaaaccat aagaaatggc aaggcaaatg
    24121 gtatagcata agtggtaaaa gtacgaagta tccctcttta aaggcaatta ctggatgggg
    24181 tcaaggggat ggattaggag gctggaactg ttcgcatcaa ttccatgcgg tcattcctgg
    24241 catatctgtt cctgcttata ctaaagagca attaaaaaat atagacccgc ccgatattgg
    24301 atataaggga agaacttata cacattacca ggctttacaa tatcagcgaa aaattgaaac
    24361 atctatgagg cagactaaga gacaactaat tgcttatgag tctgctggtc ttaaagatga
    24421 gtttacaagt gcaagcataa agttgcaaag gcagaaacaa gagtatagag agtttagtaa
    24481 ggttgctaaa ttgagattgc aaaacgatag acaccaggtg atgggatata ataaaagcgt
    24541 tagccaaaag gctgtgtatg ctggtaaaaa aggagagaaa aaatgaataa aaattcaata
    24601 actcaacaac aaatagatga tttatttaat caatctgaga aaaaagtgga tgtatattgg
    24661 ggcaaatgta caattatgac aattcagttg tccaatggat ttaccttagt agggcatagt
    24721 gcttgtgttg accctgcaaa ttatgacgaa caaattggat tggatatttg cgaggaacaa
    24781 attaaaaacg agctttggaa gttggagggg tataggcttc aatgcgaatt aggtaaattg
    24841 taatatttaa aaggttagaa agtcttatat taataagact tatttttatg cttaaaaata
    24901 cttaattcat ttatatagat gtctactaag cgacgtaaaa agttagacaa tatgtgatgc
    24961 taccacgtaa aaaagcgtaa ttgtaaaaaa caggaggttt ttatgaaaag agagtttttg
    25021 aaaagtttag atttagagga cgaggttata gaaaaaatca tgtctgagaa tgggagggat
    25081 atagaaaagt ataaaaaaga agtagaaaag aaaaaagaag aattggaaag caaaaacact
    25141 gaactcgaaa cagctaataa taaaattaaa gacttagaaa aaatagatgt tgagagtata
    25201 aaaaaagagg ctaatgactg gaagagtaaa gccgaacagg ctcaaaaaga taaggaattg
    25261 attgaaaatc aaatgtcaga gcagacttat aacttaaatt tagataatta tttaagtaat
    25321 tttaagtttt ctagtaatct ctcaaaagag gctgtaaaaa taaaaatgaa agaaaaaaga
    25381 cttgaatata agaacggggc ttttgaggga gcagatgatt atatgaaaga attgcaggca
    25441 aatgacccag gggcttttgt aagtggcaat agtgttccaa aggttgttag ttctagcagt
    25501 ggagatgctg gagagtctaa agtaagttta atggagcaaa tgatagcaaa aaatagagaa
    25561 atgttgaaca tataggaggt agaaaatgag tttatttgat tcaaaaattt ttaatggaga
    25621 agtgtttgga aaatacgttg agactgtacc taatctaaat agggacgaat taataaagtc
    25681 tggagcaata agaagaaggg acgatttaaa ggctatgttt tcagcccaat cgggagcaaa
    25741 ttatgcaact attccaatgc ttggcttgat tgatggcgac ccagtcaatt acgacggaca
    25801 aacggatata gtagcaacta caacaaagac cttttcgcag tctgtaattg ttgttggtag
    25861 agctaaatcg tgggtggaaa aagactttag ccaggacgta accggcggag ttccttttat
    25921 ggagaatgta ggaaatcaag ttgctaaata ttgggataat atagaccaaa aaactttgtt
    25981 agctgttctg gaaggtattt tcgcaatgac aggggcgaaa aatttagaat ttgttaacaa
    26041 tcatactttt gatataacaa aagaagcaga aacagaaaat caaaaggtta aagccgaaac
    26101 tcttaatagt gcaactcaaa aagcttgtgg agataataaa gaaaaatttg cacttgttat
    26161 aatgcacagt gttatatcaa ctaacttaga aaatttaaat cttgttgcta ggttaaaata
    26221 tactgaccca caaggtattc aaagagaaat gaagttagga acttggggtg gcaaattagt
    26281 cttaattgat gacaatatgc caactagaga ggtcgctgaa agttcagagg gttcaaatga
    26341 tggatacaca gagtatacaa catatgtgct tggggaaaat tctattgact tcgaggatgt
    26401 tggtgcaaaa gtcccatatg aaatggatag agctccaggt aaaaatggtg gagaagatta
    26461 cttatatagt agacaaagaa aagtatttgt gccatatggt attagcttta ctaaaaaagc
    26521 agttgcaaca gcatctccaa ctgattcaga actgaaaaac ggagcaaatt gggagcttgt
    26581 gaatgatagt tcggatgggt ctaaaactta tatagaccac aaagctatac ctattgctag
    26641 aattatttcg agagggtagg ctatgaatat gtatatagac tatgaatttt atagcactct
    26701 tgggggagaa atcccccaag gtaaatttaa taaatatgca ataagagcaa ctaaatatat
    26761 agattataat acttttaata gaatagaaga agttacagaa gaaataaaaa tagccacttg
    26821 tgagattgct gacctcatat ataaaagtag cctagaaggc gataaggaag ttcagtctga
    26881 aagtattgga agtcattcag tgacttattc agctaacaat aaaacgatag agcaaaaagc
    26941 atatgatatt ttaaaaatgt atttagattc agatttgctg tatcgaggtg tttagcgtgt
    27001 tttttaaaga taatataact ttatataata aatattatga taagtactct gacaaaactt
    27061 tatacaagag ggcttattta tttggagtag attatcaagg ttctaaaaat atagctgtta
    27121 cagataaggg gctgttaagt gctgatagtg ttaaggttat agttcccttt tctgcaaatg
    27181 caagagggaa gaagtatata gaccctttca aatattatga attagatgaa atcgaaaagg
    27241 aaaaatttta tactttcaag gttggcgata ttattgtaaa agaggtcgta gactttgaaa
    27301 taactagcgt taagccttat acattaagag aattacaaag taaatttgat gatgtcagta
    27361 ttataaaatc agtcattaaa tgtgattttg gaagcatgaa aatgaggcac tttgaattgg
    27421 aggcagaata atatgggatg aatgtaagtg taaatataga ccctaataaa ataatgaggg
    27481 caaggggact agaacctggt ggcaaggcac agaaattttt tacacatgaa gttagaaggc
    27541 tttcagaccc gtatgttcct atgttgaatg gagtcttgaa aaatacagct agagaagaag
    27601 ttaataagat aaaatatata caacctcatg caaaaaaaca atattatgaa aatgccggac
    27661 gaggcaaagg agggactagc aatggaggaa aaaggggaaa gtactgggaa aaaagaatgc
    27721 tggcagacaa taaagacgga ttaatagaat ctgttgctaa gtttattgga ggaaatagag
    27781 aatgaccata attgaaagtg ttagggagtt tataaaaaaa tgcccctatt tggacgaatt
    27841 tgcaaaaagt ataaatgttg agtttttagg tgaggaattt acttcttata cattagagac
    27901 tgttccatca gagacagtca ttaaaagatt tataaatggc gactctataa aacagtttat
    27961 atttatattt gcaagtagag agtgttatgg gtctgatgtt atgcagaata tagaaaatag
    28021 ccagttctac gagcaatttg cagactggat atatagagaa aatttaagtg ggaatttgcc
    28081 tgctttatct ggtaataaag aggcaatgtc actagaggta agtacgccag gctacccagt
    28141 tcaaacaagt atagatacag cacaatatca aatacaatta aaattaaaat attttgaaaa
    28201 aggagaaatg taaaaatggc aggaatggaa acgatacaaa gatatagagt agcagactac
    28261 ttaggagttt tggggggtga tggacaaaat tcttgggtat taatgggagc aggatttaat
    28321 acattagacg aaaatccatc cgcacaaagt gactctaaaa catatataaa cgatagaaac
    28381 agtactagcg ctatcaaatc ttatcaaacg caattttcgt ttgaaactga tttaattaaa
    28441 aatgaagagg caataatgga actttacaaa ataggtagag accaggcaac tggcgcagat
    28501 gctgaaagag attatttaag agtagaatta tttttaccaa ttgaaggcaa agaaaatact
    28561 tttagggcta gaaaatttag agtgtctgtt gaagtgagtg gattcgctgg tgcaggtgga
    28621 gaaactatta cagtgagtgg aaatttaaat accgttggcg accttgtaca tggagaattt
    28681 aatactcaaa caagaacttt tgtagaagat ggagcagaag ccccgccagc tgaaagttta
    28741 aaagttttaa ctataaattc agtggcaggt actagcacag ggaatacaaa aataacagtt
    28801 actccaaccc ttgaaagtgg gaatagctat aaatataaaa ctggggcaaa tgttgcggtt
    28861 ccaacgcttg atactgactg ttcagttggg tacactgctt gggatggtac ggaagaaata
    28921 acagcaacta agggaaataa aatattaata gtagaagtta atgctaataa taaagctaag
    28981 aaagctggta ttactacgat tacagtaaaa gaataaaagg agactaaaga aatgataatt
    29041 aataatatag aattagaaga ttttgatata tatgatgctg atgaagctga aaaagttgaa
    29101 aaaacaataa gcttaactat tactgaaacg ggaaaagttg aaaaagaagc gaccaagggt
    29161 tatgaaatga ttagagatat atgtaatttg atttttaaat gttttgatga tatttttgga
    29221 gaaggcacat caagcaaaat gtttagagat aagagaaatt taaaagtgtg ctcaaatgct
    29281 ttttttgaat tgatagaaga agttaataaa cagaaacagg agggggaaga attttttgct
    29341 tctgcaatct caaaatacac accaaacagg gcgcaacgaa gagctaaaaa ataatgaata
    29401 tgttagtcga tatacttccc gaaagtgttg aaattgatgg gaaagagtat aaaattaata
    29461 cagactttag aatatcaatt ttgtttgaaa tgttaataca agataattct ataagtgatg
    29521 aggagaaggg ggaaaatgct cttcttctat attaccctgt aatccctgca aatactacaa
    29581 tggctataga taaaattatt tggttttatt cgtgcgggaa aaaagaaaac aatgtggatg
    29641 atggttatca aggtggcgca gggtctagca aaagccaaat atattcatat gaccatgacg
    29701 acgagtatat atattcagcc tttttaggtc aatatgggat tgatttgcag gacatagaaa
    29761 aattgcattg gtggaaattt aaagcattgt ttaagtcttt aaaagaggac accgaaatag
    29821 ttaaaatcat gggctatagg gcaatggata taagtggaga tatgccaaag gctcaaaaag
    29881 atttttatag aaaaatgaaa aagatacatg ctataccact tcctcaaaat gaggttgaaa
    29941 aaataaatga aattgaaaag gcactgttga atggtggaga tattagtaat ttgttataaa
    30001 atagcatact ctacacttcc cgaattagag tataattaat atataagtat attcgattta
    30061 ggagggatta ctatgggctt taaatttaga aaaagcataa atttaggcgg aggattaaag
    30121 ttaaatatta ataaaaatag tgttagcata agtgggggcg ttaaaggtgc tagggtttca
    30181 gttaattcca aaggtaaatc aactactact attggagtgc ctgggactgg tttatattat
    30241 caagaaacaa ctcaactagg tggaggtaaa aagaagaata gtaagagtgc aggaggttca
    30301 gagagtagta ggcaggctca acagctatta aaaataataa acgattgtgc taatatagtt
    30361 aatactacaa aagaccctaa gacatttttt tatagatata atatgttgtt agataagtca
    30421 tatgccttag ttgccataga ggatgattta aacttttcag gtcaatctcc tagtcagatg
    30481 ctagacagca taatagcaaa aaagactgat actataaatg actttttaac tagatactac
    30541 aatgaaatgt ctagtaaatt gaaagaacta aaaactgaaa aggctcgcat gaacaatgct
    30601 tataaattct ctagcttagc aatagattat aaagaacatt taaataatga taacaaagct
    30661 aagcttaata gactatataa acaactatta gatgatgtta aaagacaaga ttaatattta
    30721 taaaaagaaa aatataataa aagatttaaa taaaaagcac ttacttttta aaaaagtagg
    30781 tgctttttta atgcaaattc ataaaaaaag ggggtggata gatggcagac ggttctatta
    30841 ttatagatac tcttttaaat accgaagacg cagagaaaca acttaataac ttagctgata
    30901 ccatgaaaaa gaaggcgaaa agcgctttag cgatagctgg aattggcgca agcttaggag
    30961 ctataggaaa acaggctatt gatttcgggg atgaatatca aaaagcaatg aatggtttcg
    31021 agtcggcgac aggaaacgca gaagctaggg caaaaggttt tggagaagcg ctgcagcagg
    31081 tttatgcaaa taattttggc gaggatatgg gcgatataag tgaggtcatg agtttaatat
    31141 ctcaaaaact cgaagggatt gacgcaagta atattcaaga agtgactgaa agcgcaatta
    31201 tgatgcggga tactttcgac atggatattg gggaaagttt aaatggtgtt aactctatga
    31261 tgcgccagtt tggggttagc gcaaaagagt cttataactt gattgcgcaa ggagcacaag
    31321 aagggttaaa tcagaatgac gacctcggcg accaattggc agaatattcg acatattacg
    31381 cacagatggg tttttcagca gaggaaatgt tcaatatgat gaaaaacggg gcggagagtg
    31441 gagtctatca aatagactat ttaaatgatg ccttgaaaga atttaatatt agaaccaagg
    31501 atggttctaa tggtactaag gaagcttttg aggcgctcgg ttttaatgct gatgaactaa
    31561 ctaagaaatt tgcaaatggt ggaagtagtg caaaagaggc ttttattgaa gttacaacag
    31621 ctttgaataa tttagatgat aatgtattga aaaatcagat aggcgtacaa ctctttggta
    31681 ctaaatttga ggatttggaa gcggacgcag taacagcgct aacaaatatt aaaggaagta
    31741 taagcagttc aaaagataaa ttagaagaaa ttaataagat aaaatataat agcttcggag
    31801 aagcagtaac gggaattgga aggcaaatgc aggttaattt acttcttcca atcgcagaag
    31861 gattgcttcc tgttttaaat gatttagcaa atagatttgc ccaggcattt cagaatgaga
    31921 atactaaaaa tgctataaga agtacagcag aaagcattgg cgggctaatt ggaacagttg
    31981 taaaattaat atctttagca ttaacccctt taattaatgt tataaccttt ttgattcagc
    32041 atggtagcct tgtagcgcct ataataactg gaattggggt tgcttttaca agtcttaaag
    32101 tagctgggat tgtgaggggt atacaaaaaa gttttacaga tgcccagctg gcggtaagaa
    32161 ttttcacatc tggtgtgact gagtcagaaa ctaaattgac tttgtatcaa atggtagttg
    32221 gattggttac caaaaaaata agtttggcgc aagttgctac caatctgtgg aaaggagcat
    32281 taactgcgct tggtggtcca gttggcttaa ctatagttgc ggtaactgca ttaacggcgg
    32341 tcgttgtttc tttatggaaa aataacgagg ctttcagaga cgcggttata aaaatttgga
    32401 acaatattaa agacgctgga gctaaaatat ttggtcagat tgctaaattc tttacagaaa
    32461 caataccgca agcactgagt aaggctatat cctttattaa aggtaattgg caaggattat
    32521 tactaatgct ggttaatcct tttgttggag cttttaagct gatttatgat aattgcgaaa
    32581 gctttagaaa taaagtaaat tctattttta ataaagttat tgcagtacta aaaaattctt
    32641 tgaattttat taaaaacagt tggcaaaaaa tcggaagtat gctaataact cctttcgtaa
    32701 atggatttaa gtctatatac aatagttgta taagttttaa aaataaggtt tttagctttt
    32761 tctctaatgt tgtaaaaggc ttcgttactt ttggaaaaaa tataataaca ggaatagtaa
    32821 atggactttt aagtggcatt ggtttagtta tagatactgt taaaaatatt ggctctcttg
    32881 tagttaatac atttaaaaaa gtactaagaa tacattcacc atccaaagtt acaacagagc
    32941 ttggagagtt tgcaggtatt ggttttgcca atggtataaa aaatacaagc aaagatgtag
    33001 tagaagcaac aaaagaatta ataagcgcta tggaaaaaaa tctagagacc aataaaagta
    33061 aatttgaaaa aatttgcgag gctataacta cagcactaaa aaatcagtat gaaagtcaaa
    33121 aagatgtaca attgaaggcg cttgacgaaa gactaaaggt cgaggaaaag gcatcaagtg
    33181 acaggttaaa agtctatgaa aaagaatata atgagaaatt aaagtattta gatacagaaa
    33241 caaatgaaaa aactaatgct atacaagaac aaattgacgc tatagacaaa caaattgaag
    33301 aagaacaaaa agcagaagaa gagaaagcgt acaatgaaaa aataagcgac cttgacagaa
    33361 aactagcaac agctaaaaat caaaaagaac gagaaaaaat acaaaaagaa atatcagaag
    33421 cacaagcaga tagacagaag aaattattag atgaacaaag gcaacaagaa aaaaacagat
    33481 taaaagaaca gattgaaaat ataaaaacag aagctagtaa caaaaaagag cagtacaaag
    33541 aagagtacga caatcaaaaa gaagcagaaa gtaaaaaact tgaattgatg aaagagtcta
    33601 atgctagtca aaaagaagaa attgaaaaat attttagtga acttttagaa gagacaaata
    33661 ttcaaaatga agcgagaagg ctacttttac aaaaaaattc agaagaaatt ataaaacttt
    33721 taagtgaata taatccgcac tggcaagatg cagggcagag tttagcagat agtttgctaa
    33781 atggagttaa ctctaaaaag caatctatcc aggaggctgt aaaggaagct attaacttaa
    33841 aagagattat accagttcaa gaaaaagagc ttgatagatt aaagaaaaag ttagaagaat
    33901 atgagaaact aaaagaaaaa gctagcgctt ctggtgcaag tggagggaat gtagactcaa
    33961 ctgccaaaag ttcttcttta gattcgagtt ctgccaaatt ggatgctgga gaactagaag
    34021 aatatgctgg tggcatagat gatgttaaaa cttctgttga aggcttagac cttatcagtg
    34081 atgaactcgt gacaggaaca gtccctaaaa ttggggattc agctggtaaa ttatctgaaa
    34141 gtataaaaaa agggttcaat ggcattggtg gtttttttgg tggaatagaa aaatggctaa
    34201 gagatacaga caaaaatata aataaatggt tggctagtgc aaaaaattct attggcgatt
    34261 tcttctctaa aataaaagat aaattcaaag aaggaattga aaatataggt ggattttttg
    34321 gcggtcttgg aaataaaata agtgaaggat ttgcgggagc tggagacttt tttacagagc
    34381 ttggagataa ggcacagcaa ggttttgcaa gtataaaaga taaagctata gagaatttca
    34441 gcggtcttgg cgaatggttc aaagaaactt ggtcaaaaat tggagatgcg attggaagcg
    34501 gattaaaaag tggtttaaac ttttttacta gcacagtgcc agaatggttt gcgggtatcg
    34561 gcgacaagat aaaagaggaa tgcgggaaaa taggagggaa aatatcaagt ttctttaatg
    34621 agacaatgcc agctataata aataatattg tcgaatggtt taaacaaatt ccatataata
    34681 ttggttttat aatcgggtct attgctggtt ttttcgttga tttaggagct aatctgtacg
    34741 cctgggcaac agagacatta ccagaaataa taaatagcat tgtcgaatgg tttgcaagcc
    34801 ttccggaaaa aatttcggaa ttttttagta tcatattaca aaatattcaa atttggggcg
    34861 aaaatttcaa ggtttctgtt tcagagtttt ttagcacaat ttgggaaaat ataacacaat
    34921 tttttacaga gctacccgaa agaatttcgg aatggtttaa tacagtagta gaaacagtaa
    34981 gtggctgggg cgaaaattta aagcagttgg caacagatat ttttaatcaa tttgttgaca
    35041 atgtattaat tccaatttcg gagcttccag gcaaattatg ggaaaagttt acggatatca
    35101 tgggaaaagt ttcggaatgg ggttctaatc taattcaaaa agcattagaa acaggaagca
    35161 aattcttaga aaatataatg aaattctttt cagagcttcc gggaaaaata tgggaaaaat
    35221 taactaatat aatgcaaaaa gtcatggagt gggaagttaa tttatctaaa aaagctttag
    35281 aaacagcgaa aaactttttt aacaatatat acaacactgt aagccagctt ccaggtaagt
    35341 ttaaagaatg gttggacagc attattaata atgttataag ttgggggact aacttagtca
    35401 aacaagctgg agaagctgga aaaaatatgg caaatgctgt taaggatgct ttgaaggatt
    35461 tacctagcaa aattatgagt atcgggaaag atgttgtaag aggcttgtgg gaaggtataa
    35521 cgggcatggg tggctggtta aaagaccaag tccttgattt tgctggtaac gttatagatg
    35581 gatttagaga cggctttgga gtacattcgc cttctataat tatgcgagat ttaattggta
    35641 gaaacctcgt aaaaggggtt ggcgttggaa tagatgtaga aactccagga ctgaaagaaa
    35701 aaatagagaa aaatatatct gaacttacag gtaaattaaa agctacagtt aattttgaaa
    35761 cgtctaaaat gcaagctaat atagttgcta gtacagattt taaagctgga aaggaaacag
    35821 ctataatggg taacaataag actgatgaag ctaatgaaaa tcaagcaggc attaaactta
    35881 atatagaaaa ctttgtaaat aatagacaac aagacataga aaatcttttt gatgaaatat
    35941 tgttcctagc aaaaagaaaa ggagttttat aaaatgctcc ttttcttttt tatatttcct
    36001 tttatctttt tttaaagact atagtaaaaa aataataaat gagaggaggg tgtaaagcta
    36061 tgtttttcgc ttacgacggt cgagatagca gagaatttgg tttaaaaata tataatataa
    36121 atgacttatc agctccacag atagaagttg aaagagtgag tgtaccaggt aaggacggag
    36181 atttattatt aaaaaaaggt tttggaaatt ttacgcttac aatagaatgc gacatagatg
    36241 caaggcaaag caatatagaa gaagttgcaa cagaaataaa aaaatggttg caaggtgata
    36301 tatcatataa aaaacttttt ttaagtaata gcgactttta ttatctagca agctgtaaca
    36361 ataaactaga tataactaga aattttaaaa actttgcatc atgtctttta acattcgact
    36421 gctacccttt tagatacaca gaagaagaaa ttataagttt aaatgtatta aatctaaaga
    36481 gtgctactat aacaaacttc tatagagagt caaaacctac tctctatata gaaggaacag
    36541 gggacataag tataaaaata aatacccaaa atatagtgtt aagaggggtg gcagaaaatg
    36601 gaattttaag cgacttaata atagatagcg aacagatgaa tgtatataga ataaacaaag
    36661 aaaataatat tattgtaaat gaaaataata agctttttag tgactttcca attcttgaag
    36721 aaggagaaaa tctgattagc tgggaagggg atataaaaag cataaaaata aatcctagat
    36781 ggaatatttt ataatttttt aattaaaaac atggagggag ggattaatat tcgaaaagga
    36841 aaattagttt tatatgaaga aaaagaaacg aatttcaaac acatgggatt aggtgttctt
    36901 acaaatgtaa taaatgacca tgttagagag gaagagagcg gagttttcga gttagaattt
    36961 actatctacg aagactcttt tttatttaaa gaaataaaaa ctgatagatt agtaaaagct
    37021 gacgcgtctc cagatttcaa aaatcaactt ttcagaattt attatatatc aaaaaatctt
    37081 ggcggatata ttaatgtaaa agcgcagcat attaagtatg atttgcttaa taatttcata
    37141 gaaagcttaa aattgaatga tattacttgt gaagacgctt tagaaagagt ctttcgtgct
    37201 tgtgaggaac agaacagatt cagaggacat tcagacatca aggcaaggaa tagtattaat
    37261 atatcaatgc aaagccctta caatgcaatc tgtgagggcg aaaattctct aattcaaatt
    37321 tttgaaccaa gtgcgaaact tttctttgat aattacgatg ttttcttgaa ttatcagcga
    37381 ggggaaagta aaaatgtctt attagcgtac aaaaaaaata tcactgggct tgaagctgaa
    37441 tacgatactc aagatattgt aactaaaata tatccttatg cgacctatga tgatgaatta
    37501 attacactat cagaaaagta tattaccagc acgagactaa acgaatatgc tacacaaaaa
    37561 atagttgcta tagattttag ttctgacgag gtcagtgttg aagaagagct aagagaaaaa
    37621 tgcaaagatt attttaaata caatcaagtt gatttaccga aagttttata taaagtcaac
    37681 tttatagact tgtcaacaac tataaattat aaagattata aaatgttaga aacggtcaat
    37741 ctaggcgatg aagtaataat acgagatttt aatttaaaca ttaacgcaac tgctagagta
    37801 gttaaaacag actatagccc aattcaacaa aaatactata gtattgaggt tggggactta
    37861 ataaatcatt tagacttttt aaataacaag tttaataata tagaaaataa aatagaaaca
    37921 gtaaaaaaag ctattgataa tgtcaaagta gatgattcag agtttccgga cactttacca
    37981 gatataccga ttttgacagc agaggggtta tttgctttta tcaatctaac atggacattt
    38041 acaaataagt cttattataa atatgaagtt tatgcgtctc aaattaaaga ctttgcacca
    38101 gatacaatta attttacaaa tcgaattttt gtagggcagg caagtgctta cgttcaccag
    38161 gctgaacctt ttcaaacttg gtatttcaga gtaagggcag taaactcgca tgcaaatgtt
    38221 acagagttct ctaatcaagt agaagcacag actacaaaaa ttgatgacgg aactacatgg
    38281 atagaaaaag gagcaatagc agatgcgctg attggagaat tgaagttaga tagaggttgg
    38341 tttggacagc taaaaggcgt atatataaat gctagagaaa tggtagtcgt aaatgacaat
    38401 ggagttaaaa caatggaaat tgatagtttt ggagatattc attttaaccc aaacactttt
    38461 aaaattgctt ttaataatat aagtccatat gttcttattg atgatgaagg gcttaggctt
    38521 ataagcggta agggatttac aaaaatgacc gactcgggac tatatacaaa attctatact
    38581 gattcagatg taacggagtc gtggtatttg caagaaagtg gatacacata ctttgactat
    38641 aaagattcaa aaatcataat agacttacca aaaaaatttg caggtaaaaa ttttaaagca
    38701 acttgtgcaa taaggtctac atcaacaagt ggtagctgtt taagttattt cagcacatca
    38761 gcgacagtaa aaaaaagtga tacaaaacca caattggaac tttctgcatc tgtgcgaggt
    38821 gtgtcttacg aaatgtcagg aagccaaaca ataggctact ggctcggaat gaattttttt
    38881 tcagaaggat ttattacagt acaatactat gtttacgctt aaaattttca aaaaaaggag
    38941 aagaagtatg gttactattt tttacagcaa gagaactgga gaaatatata attgcataaa
    39001 atcagaggta gagcaggatt atacttgttt tgcggatagg gaagaggatt atagacaaat
    39061 attagataaa atagttgttg aggatattcc agaaatttta aatcctcgtg attataaaat
    39121 tgaaaacgga aaatttgtat taaaggaaaa agaaataata aaagaattta ctccgaaaat
    39181 ggtagagtaa aaggagtgat aaatttgaga gatagaattt atacagtaga tattaacact
    39241 aaaagttacc aagttgcaaa atataagcaa tatgataatg caatagaatt taagattaat
    39301 ttattagaaa ataatataga aaaagatttg acaggatata cagctatagc aaattttcaa
    39361 agaccagacg ggaaaatagt ttatcagagc tgtactatag aaaattctat tgtaacaact
    39421 ataatagaaa ataacataac agcagttgcg ggggatgtaa tagtagagtt tactttctac
    39481 aaagatgatt tagttgttac tactttttct ttaaagatta atatagagaa aagtatagat
    39541 aaaaacagta ttacagaaga gccgaaatgg gattatatca gcgttacaat aaatcaagta
    39601 aaagaagtcg tcgaaggcat agaagaaata aaagaaacag aagaagcaag aaaagaagca
    39661 gaaattaaaa gggtcgaaga atttaatagt ataaaagaaa ctttcgactc taaagttaca
    39721 gaagtcacag acgctaaaaa cag
//
LOCUS       DAGQWR010000016        38901 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.016, whole genome shotgun sequence.
ACCESSION   DAGQWR010000016 DAGQWR010000000
VERSION     DAGQWR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.016"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(201..1238)
                     /locus_tag="MIW95_000757"
     CDS             complement(201..1238)
                     /locus_tag="MIW95_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBY3150105.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(1257..2042)
                     /locus_tag="MIW95_000758"
     CDS             complement(1257..2042)
                     /locus_tag="MIW95_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBY3150106.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(2075..3208)
                     /locus_tag="MIW95_000759"
     CDS             complement(2075..3208)
                     /locus_tag="MIW95_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBY3150107.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(3256..4383)
                     /gene="hadC"
                     /locus_tag="MIW95_000760"
     CDS             complement(3256..4383)
                     /gene="hadC"
                     /locus_tag="MIW95_000760"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBY3150108.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKNETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(4383..5609)
                     /gene="hadB"
                     /locus_tag="MIW95_000761"
     CDS             complement(4383..5609)
                     /gene="hadB"
                     /locus_tag="MIW95_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBY3150109.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(5624..6424)
                     /gene="hadI"
                     /locus_tag="MIW95_000762"
     CDS             complement(5624..6424)
                     /gene="hadI"
                     /locus_tag="MIW95_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBY3150110.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(6463..7662)
                     /gene="hadA"
                     /locus_tag="MIW95_000763"
     CDS             complement(6463..7662)
                     /gene="hadA"
                     /locus_tag="MIW95_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBY3150111.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            8339..9337
                     /locus_tag="MIW95_000764"
     CDS             8339..9337
                     /locus_tag="MIW95_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBY3150112.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            9699..10226
                     /locus_tag="MIW95_000765"
     CDS             9699..10226
                     /locus_tag="MIW95_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBY3150113.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            10253..10849
                     /locus_tag="MIW95_000766"
     CDS             10253..10849
                     /locus_tag="MIW95_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBY3150114.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDTVSISLNAPNKEAYNRVTQPKFGEKSFDYMLGFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(10914..12497)
                     /gene="asnB"
                     /locus_tag="MIW95_000767"
     CDS             complement(10914..12497)
                     /gene="asnB"
                     /locus_tag="MIW95_000767"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBY3150115.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(13063..13899)
                     /locus_tag="MIW95_000768"
     CDS             complement(13063..13899)
                     /locus_tag="MIW95_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY3150116.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKKFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(13923..15389)
                     /locus_tag="MIW95_000769"
     CDS             complement(13923..15389)
                     /locus_tag="MIW95_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBY3150117.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIAKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(15392..17254)
                     /locus_tag="MIW95_000770"
     CDS             complement(15392..17254)
                     /locus_tag="MIW95_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY3150118.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            complement(17501..18364)
                     /locus_tag="MIW95_000771"
     CDS             complement(17501..18364)
                     /locus_tag="MIW95_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBY3150119.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(18419..19351)
                     /locus_tag="MIW95_000772"
     CDS             complement(18419..19351)
                     /locus_tag="MIW95_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBY3150120.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            complement(19437..19988)
                     /locus_tag="MIW95_000773"
     CDS             complement(19437..19988)
                     /locus_tag="MIW95_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3150121.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            complement(20217..20708)
                     /locus_tag="MIW95_000774"
     CDS             complement(20217..20708)
                     /locus_tag="MIW95_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150122.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(20736..21296)
                     /locus_tag="MIW95_000775"
     CDS             complement(20736..21296)
                     /locus_tag="MIW95_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBY3150123.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            complement(21448..22605)
                     /locus_tag="MIW95_000776"
     CDS             complement(21448..22605)
                     /locus_tag="MIW95_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBY3150124.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRHFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(22642..23583)
                     /locus_tag="MIW95_000777"
     CDS             complement(22642..23583)
                     /locus_tag="MIW95_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150125.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            complement(23670..24362)
                     /locus_tag="MIW95_000778"
     CDS             complement(23670..24362)
                     /locus_tag="MIW95_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY3150126.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(24472..25284)
                     /locus_tag="MIW95_000779"
     CDS             complement(24472..25284)
                     /locus_tag="MIW95_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBY3150127.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(25335..26246)
                     /locus_tag="MIW95_000780"
     CDS             complement(25335..26246)
                     /locus_tag="MIW95_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY3150128.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEVGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(26305..27036)
                     /locus_tag="MIW95_000781"
     CDS             complement(26305..27036)
                     /locus_tag="MIW95_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBY3150129.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            27125..27376
                     /locus_tag="MIW95_000782"
     CDS             27125..27376
                     /locus_tag="MIW95_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150130.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGTKFINKKR"
     gene            27609..28028
                     /locus_tag="MIW95_000783"
     CDS             27609..28028
                     /locus_tag="MIW95_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150131.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSPINLKSGDIFTFIRLTLLAGRMF"
     gene            28053..33110
                     /locus_tag="MIW95_000784"
     CDS             28053..33110
                     /locus_tag="MIW95_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBY3150132.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVTSALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNSDRQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTSEDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKFKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGIDYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDESGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQLVQPFKQVFREYYRINSDEISEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNINFEGSHV
                     LITGNLGEYSVHMGSGIVHKKGMGALNILPVHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDNKIKDPSILSNL"
     gene            complement(33270..34007)
                     /locus_tag="MIW95_000785"
     CDS             complement(33270..34007)
                     /locus_tag="MIW95_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3150133.1"
                     /translation="MIKVLICDDDKIVRDDIANIVKESGYVDYVKKVKDGIEAIEFIK
                     SEKIDLLIIDIDMPYKNGIDSAKEILSIDKDIHIVFVTGFADYSLESFKVHPIDFIVK
                     PFKKEKILDSINIAIDHINSHKIAKNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLYTRDEDTIKFYENFDDLKKRLPPNFFMTHRQYIVNLRLIHKVIPINKSSLEIKF
                     INFQESATLNKSLEKDFLYRFYRVKRF"
     gene            complement(34026..34961)
                     /locus_tag="MIW95_000786"
     CDS             complement(34026..34961)
                     /locus_tag="MIW95_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBY3150134.1"
                     /translation="MENNFIFPIVILTLFIFSNLIMNKYRFYKELDILKRGILTFFVS
                     CFVWILFMEGIEYIQLSLGYNFLLEGVLNIILFLILIILSFYIGFKFIRLLDNNNKQE
                     YKLQKLDSKINHKDNIIVKLRSQKHDYLKHLQVVYSLLNTGLAEDAKEYITNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKNISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSAIQKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFLDGFSTKEGQDRGF
                     GLYLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            35169..37754
                     /locus_tag="MIW95_000787"
     CDS             35169..37754
                     /locus_tag="MIW95_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY3150135.1"
                     /translation="MKDNLKIALRYIISYKARSLAIALSIILATSLIVGIGTLSRSAQ
                     QAEVDKLKRETGSDHVYFKDINKNQLEYIKSRKDIKNLGITSHYGYTDPNERLAINLE
                     YANKNYLTNQSKLIKGHLPKASNEVVVEKWILNSLGLKPEINQNITFKLYQKEKPETF
                     KVVGILEDRPIEKNKGTCEIFLNLNESKLDKFSYAYIEFNNGIDINTSIDNIVKNTML
                     DENSVGKNKMLIESTRENGTLDNSSKYTAITMSLFSGIVIYSIYVISIYQRIQEYGIL
                     RAIGSTNFKIFKFMFYELFILALIAIPIGICTGIGGAQIFNRTAGNIQFEGNVTVTPF
                     VIPDKIILLSIGSIILTILIISFFTYLKIRRISPIDSIRKTFGTDKNINKVNSLISKL
                     TLNISVTKSISAKNIFRNKKGFIIIILSMSLGGIMIIKENYKYSFSDIQNKNGQEETY
                     MNADFILTNFFLKLNQSNKADSFKDIKGLNDNQIDKIKNINGIDKVKTASILDTRIEV
                     DKLNGLDYYNIINSTPYYKDFPLFIKNKNTGKYTMKQKLRGYNDEMINSLEKYLVSGS
                     INLERMKKENLAILYVPQVSKTNKYKLSYTPGTETPAVDIKVGDTIKVKYPKGEIDQD
                     LYIKLKDNYEYLEYEFKVGAIVSYPFADNYLYSGDQGIDVITSNDYLKKLTGVDKYNV
                     VYVDINKNANVKKINTLLGEIGSESPGTTTANMLMEKENFDKMTARALTYAYGIVAVM
                     FIISVFNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGILSSVITIVV
                     GLIIQFRMYYTYNFVSYGLGFSIDYKIYILVVLANIIVGILATYIPLRKINKISIVEA
                     INITE"
     gene            37766..38437
                     /locus_tag="MIW95_000788"
     CDS             37766..38437
                     /locus_tag="MIW95_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3150136.1"
                     /translation="MPILEILNLGKIYGKKETAVHALKDANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSSGTIKVDGKDICCLSDKKLAKYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKVDEEYIEDIINLLGLSERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            38464..38805
                     /locus_tag="MIW95_000789"
     CDS             38464..38805
                     /locus_tag="MIW95_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150137.1"
                     /translation="MLLILDNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRINKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
BASE COUNT        13269 a         6381 c         4742 g        14509 t
ORIGIN      
        1 cttacagaat ataactcaca atttgtaagc aatatacata taaggattat ccagattatc
       61 ttgtattata aaattttatt caaacagact ttttaataac tgacaaatac acactagcaa
      121 taacttatca aagggttaat aattatgtta ctaactaata agacttattc aatcagtaat
      181 atttatatag tttgtatcta ttaagctttt atagcttcta ataaatcatc aacgctatct
      241 aatttttcta taagcattgg aactatttca tgtaagtctc caactactcc atagtcagcg
      301 atttctaaaa ttggagctgc tgggtttttg tttatagcaa ttatgaaatc tgatgattgc
      361 atacctgcta aatgttgtat tgctcctgat ataccacaag ctatatatag gtttggttta
      421 acagtagttc ctgtttgacc tacttggtga gaatggtcta tccatccagc atcaacagcc
      481 gcacgagaag aaccaactac tccacctaat ttgtcagcta atttcttaag catttcaaat
      541 ccttctggtc cacctaatcc taaaccacct gatactataa catttgcatc tgtaagagat
      601 actaaatctt tcttagtttt aactgtttct aaaacagtag ttcttatatc atcttgagtt
      661 attttgtagt ctaatgctat aacttcacca gttcttgttt cgtctttttc agctttatcc
      721 ataacaccag gtctaactgt agacatttga ggtctatggt ttggacaaat gattgtagcc
      781 attatgtttc caccaaatgc tggacgagtt tgttttattt tcttatcttc tgggtctatt
      841 tctaacttag tacagtcagc tgttaatcca gttccaactc ttgaagctat tctaggtgct
      901 aagtctctac ctatatgagt tgctccgaaa agcattattt ctggttttat ttcatctatt
      961 gcatctttta ttacttttgt atatgcatca gttgtatatt tttctaaaag agcatcatct
     1021 gcaacataaa ctttgtcagc tccaaaagtg attaattctt tagctaatcc atcaacatct
     1081 tttcctaata atattgcaca aagttctgca tctacttctt tagctaattt tcttccttct
     1141 cctaataatt ctataactac tggagctatt tttccttctc tttgttctgc aaatatccat
     1201 acgtttttat aagaacttaa atcttttata tcattcatag tttgtactcc tccaacttat
     1261 ataatgtgct tttgttttag accgattatt attttactag ctatttcatc agctttacct
     1321 tctaagattt ctccttttcc tcttggttca ggagtaaatg atctaaatac tttagttggt
     1381 gatgctttta aaccaacttc ttctttattt acatctaaat catcaattgt ccaaactttt
     1441 acatcatgtt tatatgcttt tactatttta tcaacagaca tgtatctagg tgtatttaac
     1501 tcttttactg ctgttaaaag tacaggtgta cttactttta ttaattcgta tccatcttct
     1561 aattgtcttt gaactattat atcttttcct tctattttga aatcttgtac atatgtaact
     1621 tgaggaatgt ctaatttctc tgcaatttgt ggtccaactt gtgcagtatc tccatctatt
     1681 gcttgtcttc ctgcaaatat tatgtcatag tcacctactt tagatattcc cgctgcaata
     1741 gtatttgatg tagcccaagt atcagctcct ccaaatgctc tatcacttaa taatattgca
     1801 tcatcagcac ccatagctaa acactctctt agcataaagt ctgcttgagg aggtcccatt
     1861 gttataactg ttatagttac atcatcatgc tcatctttaa tttttaaagc ttcctctaat
     1921 gcatttgcat catctgggtt taatatactt ggaactccat ctcttataag agtacctgtt
     1981 tctttattta ttcttacttc gttagtatct ggaacttgtt ttacgcaaac taatattttc
     2041 aaagtctata cctccaattt cattagatta tttattattt taatacgttt gatgaaatta
     2101 ccattttttg aacttctgat gttccttcgt atattgatac gattctagca tctctgtaca
     2161 ttctttcgat ttcataatct tttataaatc catatccacc gtgaagttgt aatgctttat
     2221 aagcaacttc atttgcaatt tctgatgcat agtatttagc catagcagat tcttgagcaa
     2281 ctctcattcc tgcatctctc ttttgtgctg catcataaac taatcctcta gctgcacaaa
     2341 cttttgtttc catgtcagct atagtaaatt gagtattttg gaatttagct ataggcttac
     2401 caaattgaac tctttgtttt gtatatttaa cagcttcatc taaagcacct tgagctattc
     2461 ctagagctaa ggcagcaaca cctattctac caacttctag agtacccatt gctatcttaa
     2521 atccttgacc ttcttttcca agtaagtttt cttttggaac tttaacgttt tcaaatatta
     2581 agtcagaagt ttcagttcct cttataccca tcttatcttc atgagctcct gttgagaaac
     2641 catcccattt actttctact ataaacgctg atgttcctct aagtccttta cttctatctg
     2701 taactgctat tactatagct acatcacaga atggtccatt agttatgaaa gtttttctac
     2761 catttaatat atagtggtct ccaactaatt ctgctgttgt ttgttgtcca cctgcatcag
     2821 aaccagctcc tggctcagtt aacccaaatg ctcctattaa ttcacctgtt gctatacctt
     2881 ttaagtattt ttgtttttgc tcttcagtac cattgtaaag tattggtaat gaagcaagag
     2941 aagaaccagc tgtaacaaaa gtagctgttg aagcacatct tctagctatt tcttccatta
     3001 ctatagcgta agaaacatat cctgctcccc caccaccata ttcttctggg atattactgc
     3061 ttataaattt tgcatcagct aattttttga ttaattcctt aggcattaca cctgttttat
     3121 ccatttctgc tggtaaagta tctaattctt tactaacaaa atctcttact gcttttctta
     3181 aaagttcttg ttctttgtta tataacatag cttcctccaa ttttcatatt ttatagtaaa
     3241 tttattttaa tgaacttacg ctaaactcat catctcagca aaagtttgaa tacgagttct
     3301 ggcttgttca ttattctgag tttgttggtc gatttcaaca tataaagtag gtattccact
     3361 atcttctata tcttttctaa ctaaaggata atcgtattct tctgggtcac agaatttcat
     3421 catacagaag ataacaccat ctatatcttt ctttttaact tcatctacta taagtgaccc
     3481 acgttttttc ttagggtcat aagctaatga acatccttct atgtttgacc attgtcttgc
     3541 taatctctct aacgcatcat cacctgctgg tacatctgtt ctgaattgtc ttgtttcttg
     3601 tgctaagtcg tcagctacaa ctgatatatt gttgtcttct aaaatgtcta atatatcttt
     3661 agaatcagct aatatacctg ttaataaaac tttttttcca gaacagactt cttctggcat
     3721 agcatttaat tttgctatta aatctttcac tagctcagta tgttcttctt ttctcataaa
     3781 gaacccactt ttaattacta agcttcttat tgatggtttt atagtattag aatgtttata
     3841 agctacttca acaaagtctc tcatagtttt tctatgttca ttgtaaactt ctatactttc
     3901 atgaattttt gcttcttcta tttcatatcc acaaatttct tcaagttctc tttttacacc
     3961 tttgtactca ctgattaagt attttacacc agcttcaagt tttctatttt gtgggtgtac
     4021 taatgatata tattttatat gaggtactgc tgatttccag ttttgtccta aacaaattag
     4081 tgtatcacac atacctggta taataactcc agataattca tcataagcac cttttaatcc
     4141 atattctaaa catgattgca ttattgaaca tgcaaatgca gggaaatatt gtttagctaa
     4201 atctaattct gtttgtcctc cccatatacc aactggaagc attccagctg catgtataat
     4261 ttcttctggg caataaactg ggaaacaacc tatagctttt ttaccagttt catttttata
     4321 tttttttaca gccgcatttg gattttcaac tacttctttc attttagata aaatagcttc
     4381 cattatatct cacctctatt aagttttttt ctttcttcca ttacttcaac taaaccttga
     4441 attcttgttt caaattgagc attagtgaaa gctctagggt ctgcttggtc accatcaaat
     4501 ccagcatatg gtaatccagt ttcttcagct gctcttcttt gcatttcata ttgtattaaa
     4561 ctcataagtt tacagcttct gttcatatga tagaatgctc catcacattt accctctact
     4621 aaagaatcaa ctctatattt tgtcatacgg tctaggttta cattgttaaa catagtacta
     4681 tatgctactg ccattccatc taaatcatta acttcatatt gtaatgccca agcatgtggg
     4741 taaacactac ctgtcatgtt aactccaaat ttagctaatg ttttcatctt gtatcctata
     4801 tatggccaac aaggtatacc ttccatcatt attctgtatt tttcttcccc tctgaaagaa
     4861 gatttaccag ttttcatgtt gtcttctaat tcttctataa gtaacttaaa tgcttctgta
     4921 gtttcttttt tacctctagc acaaactatt acagccatgt aagtaaataa gtcaaatcca
     4981 ttcattggag aaggagaaga atctgctggt aaactcatag aatacttcca tagtcttccg
     5041 ttttcagctg atattttcat tacttcttca aacttcttag ggtcaaattt ctttcctgat
     5101 ataatttcta gttgttttat agcttcttca aattgagctt taatataatc tattcttgat
     5161 tgagtaactt cgtcttcatt attgaaagtt gtatcaatca ttattaaagg tatatctaat
     5221 tctcttgaaa tattttcata ccattttata acttgattac atatattgtt acagcaaagt
     5281 aggaaatctg gagctggcat atccaaagct tcacatccac cattttctaa aagaccaaaa
     5341 tttgttcttg catatgcaca taggtcaata gaatatcctt tagattcagc tatttcacat
     5401 aattctaaag aacctttttt agctgctact ccagctgctt ggttttctgg atataatacg
     5461 tttaagtcaa atacttctgc taactcttga ggaaatactg atgttgacca acctacaggt
     5521 cttccttctt ctttagcttt aaatgcattt gcatattgtt cagctaataa atcattaatt
     5581 actactctag cttctttttt ttcagacatt ttcatccctc tttttatata tttttcactt
     5641 ctttttgtga ttcttttgct tcatcaaaag catataacgc tgctcctaaa gcaccagtta
     5701 attgaggtat atcaggtaca ataatttctg tgttgatttc tcttgccata gctcttacaa
     5761 taccactatt tctagcaact ccaccaacca taactacatt tctttgtact cctattcttt
     5821 ttactaggct agaaactctc tttgctactg aagtatgaat acctgccact atatcttcaa
     5881 tttttgcatt ttcagataaa tgtgatataa cctcagactc tgcaaatact gtacatgtac
     5941 tgcttattga tacttcattt tgagaattca tagatatact tccgagttca gatacatcaa
     6001 cctctattat ttttgccatt acatctaaaa atcttcctgt acctgcagca catttgtcat
     6061 tcataagaaa gtttaatagt cttccattat tatctaattt taataccttt gcatcttgac
     6121 cacctatgtc aataatggtt cttgtctctg gaattatgaa atttacccct ctagcatgac
     6181 agcttaattc acttatttgc ttatcagcat ctgaatagtt cattcttcca tatcctgtaa
     6241 ctacaacttt tttaatatct tctcttgcaa gacctgtctt gccatataat ttttctaaaa
     6301 ctcttgatgg tccagtagtc ccagtaccag aggatattgt ttcagaagct acaatatctt
     6361 ccccattctt taagattact ccctttgatg cagttgaacc tatatctaat cccattgtgt
     6421 acatctttag cattctcctt ttaaaaaatc tagaattact aattaatatc ttacaacttt
     6481 actatcttta aagttattta ttttctcttc atcgtagcct aaagatttta atacttctac
     6541 agtatgttga cctacttttg gtgctggtgt atattcttta attccttcat ttctaaacat
     6601 aactggagta ttaactaaga cacctgtatt tccgctatcg tatgttttct taaataagaa
     6661 gtcatttgcc caagcttgtt cgtcatctaa taaatcttca cagctttgaa ttttttcaaa
     6721 tggtaagtct gcttcttcta ataaagctga ccactcgtct aatgtttttt ctaacatagc
     6781 ttctccaact atcttaacta aatcttcaac atgattaacc attgaatcta tgttattata
     6841 tctatcatct tctaatatat attctctatt tataacctta cagaatttgc ctaaccactt
     6901 gttgtattgt attaaagcta attgaatcca tcttccatct ttacatttat atgtagtcat
     6961 taatgggctg tttggatttt ctcttgataa aggcatttca tttccgtatt gtgctgttgt
     7021 tatcattgtt cccattccat atatagctgt atggaaaaga cttactgtta ctctctcacc
     7081 tttaccagtt tgagcttttt tatgtaatgc tgctaaactt cctgctgcta gtgctagacc
     7141 tgcatagtgg tcaccaaatc ctgctgctgt atttgctgga gatgttcctt tttccataac
     7201 agattggcta actcctcctc ttgcgaagta tgcagtatag tcaaatcctg gtttatcttt
     7261 taaaggtcct ttttcaccat atcctaatat ttgagagaat attaatcctg gatacttatc
     7321 ttttatttgg tcataagcta tacccatttt ttctaatgct tgaactctaa cattagttac
     7381 aaatatgtct gcttctgata ataatttatg taatatttct actccttctt ttgattttac
     7441 attaatactt acaccctttt tatttccatt ttctaattca aacatagggt tttcatcatc
     7501 tgatgctgga gatttaaatg ttccacccat aactcttatt ccatcacctt ctataggttc
     7561 aatcttaata acctctgcac cccagtcacc taacattttt gcacaacatg gtgctgcgat
     7621 gaaacttgaa agttctacta ctttaactcc ttctaaaagc ataacgtttt cctccttaat
     7681 agtatcaatt ataaaatttc gattaaatca ctcataaaat acttttttct gatattaact
     7741 gtctgatgtc aaagttccta atcttatcta aaataactta aaaattatta aaattatttt
     7801 ttattgtttt attgtcttac acctatattg ttaagcattt atcgtgccaa ctttatattt
     7861 aatcaaaata tcgcttgatt tgaatgtttt ttaacaatat gcaattgaat aatctttttc
     7921 atatcgtggc atcttgccac tatccattgc attttgctat tctttctatg cttataaaag
     7981 ttttttttat atattttttt atgaaaaagc ttgacatttc aaaaaaagtt ttcattgcta
     8041 aactcaaaaa cacatgttat cattgtaatt aatcagcaag ttagttaact aatttcaaac
     8101 ttagataatt ttttaataat atttaatcta aaaaataaca atttctttga taaattttag
     8161 agtaattatt acattaaata aaaaattact aaatatcaaa ataacacagg gaaaacaatt
     8221 tcaaaactaa atttttaacc ttaaaaattt tgtataaaaa atataacctt atatgctaca
     8281 tattatttct aaatgaaaaa ttaccataat aatttaataa ttacaggagg gtattgatat
     8341 gaaaatacta gtatttggag cacgcgatta tgaagaacca gtaataaaaa aatggtctga
     8401 agaacataag gatgttcaag tggatattta tcctgaaaac atgactgaag aaaatgtagt
     8461 taaagctaaa gggtatgatg gtatatctat acaacaaact aactatatag ataatcctta
     8521 tatttatgaa actttaaaag atgctggggt taaagttata gcttcaagaa ctgcaggggt
     8581 tgacatgata cattttgatt tagttaatga aaatggactt atcgttacaa acgttccttc
     8641 ttattcacct aatgcaatag ctgaattagc tgttactcaa gctatgaacc ttttaagaaa
     8701 gactcctcta gtaaagaaaa aagtctgtga aggtgattac cgttggatag ctgaacttct
     8761 tggaacagaa gttagatcta ttacagttgg tgttataggt acaggaaaaa taggtgctac
     8821 ttctgcaaaa ttattcaaag gcctaggagc taatgtaatt gcatttgacc aatatccaaa
     8881 tagtgattta aatgatatat taacttacaa agattcttta gaagaccttc taaaagaagc
     8941 tgaccttata acattacata ctcctttact tgaaggaaca aaacatatga taaataaaga
     9001 tactctagct ataatgaagg atggagctta catagtaaat actggccgtg gtggtttaat
     9061 taatacaggg gatttaatag aagcactaga gtcaggaaaa attagagctg ctgcccttga
     9121 tacatttgaa actgaaggat tgttcttaaa caaaaaaatg aatcctggag aattaactga
     9181 cccagaaata aataaacttc tttctatgga acaagttata ttcactcatc accttggttt
     9241 cttcactagt acagcgattg aaaatatagt ttattctagt ttaagtagtg ctgtagaagt
     9301 tataaaaaca ggaactgcta ctaatagagt aaattagtat tttataaata tattttttca
     9361 ttaattagct attatctaac aaaatagcct ttaatttccc aaatataaga gagtcagctc
     9421 agtatatatt gagtagactc tcttatatta ttttttatat taaacttgta ctcctcttat
     9481 ttataccaaa ttaaatttat attaacttac tctaactact cctactctat acattttacc
     9541 tatttttact tttctaatta tatacatttg aatgggtaca atctgtattt tacaaaaaaa
     9601 acacctaaca aactatgaaa attgtatgat taatattaat atatttataa gaaaacataa
     9661 caattatgtt agctctggat ttttagggag ggagtatatt ggaaaaaatg aataaagtta
     9721 cttctaaaaa aactacctat attgttactt cagcattatt tgcatccata atatgcctga
     9781 caatagcata tatattacat attccagttg gtggaaacaa tggatatgtt catataggag
     9841 atgcttttat ttatctagct gcaactattt tgccaacaaa ctatgctata gctgcttctg
     9901 caattggtgc tggtctagct gatttatcta caggtgctgc tatctgggta ataccaacta
     9961 taatcataaa accaatactt gtattatttt tcacttctaa aagtgacaag ataataaata
    10021 agagaaatat agttgcttcg gttgtagcag gtatagtagg attagtttta tacatgtttg
    10081 ctgaaggtat aattattgga agttttacaa gtgcatttgt aatgagctta cttggcttat
    10141 tacagccaat aggaagtttt attgttttta taatcttagg tatggcactt gataaacttg
    10201 attttaaaaa acgttatttt aattaagaat ttaaatttat ggagggaaaa ttatgaatat
    10261 cttatataca attgaaaata gtatctatgt aaatataact aatacatgtc catgtagttg
    10321 tgttttttgt ataagaaacg aaaaagatga agttgcaaat agtggtagtt tatggttgga
    10381 gcatgagcca agtgtagatg aagtaaaaga agccttcaat aaatacaatt tagacgacta
    10441 tgatgaaatc gtattttgtg gatatggcga accattaatg agaataaatg aattgataga
    10501 ggtagcaaag tttataaaag aaaaaagtag cataaaaata agaataaata caaatggtct
    10561 aagtgattta atacataaca aaaaaacagc tattctgtta aaagatgtta tagatacagt
    10621 atcaataagt cttaatgccc caaataaaga agcttataat agagttactc aaccaaagtt
    10681 tggagaaaaa tcatttgact atatgttagg ttttgcaaga gattgcaaaa aatatataaa
    10741 agaagtagct ttttcagtag tagatgaaat atctccagaa gaaatagaag aatctaaaca
    10801 actggcaaaa aaacttgata taccattaag agttagacat aagaattagc aagtaaaatt
    10861 ataaaacatt aagaatcacc tgaatggtct ttcaggtgat tcttcctctt gtattaacat
    10921 tttacaaaat agatttcata ccacactagg aatgtgtaaa tcatccaaat ctttctccaa
    10981 ttatcagagt ttccactaat gtagtcatta aaaatctcat taatctcatc tatgttaaag
    11041 aatttatctg ctatctcact attgaattta tctcggatat ccttattata ttggtcatca
    11101 gccatccata tacgaattgg cacaataaat cctaatttat ttctaaaagc aatctcttct
    11161 ggtaatgttt ttgatgcagc tgtacgaaag gcaattttat tctcttttcc tttcacttta
    11221 tattctgatg gtattctgga tgcaatatca aatattcttg tatctgtaag aggcattctg
    11281 acttccaatc caactgcagt actcatttta tctatattaa aataaatatc tccttccaac
    11341 catatttgaa tatctacatt tgacatgtta gtaagtgtat ctagttcttc tgtttcttca
    11401 taaattcctt ttaccacttc aataggaagc acatcaggat tgtatttctt aagaattttt
    11461 cttttctcac cctcattcat aatgtttgta tttccaatat aaaatgtctt caaattctct
    11521 ccataacact ctgccttatg gtatatatta tatccaccaa aaaactcatc tgctccttct
    11581 ccagagtaac agatatttgt gttttcagct gctgccttgc atccaatagc aaaaacgata
    11641 gcagatgcat ctccagttgg ttgctccata tggtacatta cataaggtac tgacgcaaaa
    11701 tactcttcag gtgtaattat atatctcgaa tttttacacc caagaatatt agctgtctgt
    11761 tgtgctaatt ttgattcatc aaaacgctct tcctcataac cacatgaacc tgttttttct
    11821 atatctgaca ttgccaatat ataagatgaa tcaacacctc ctgataagaa taactctgca
    11881 gtttcattct ctgattttac ctctggcata attttctgaa gtgttgtatg aatttcatcc
    11941 gtccattcct caatagactt gctgtggtca ggatgaaatt caggtttcca ataacgacca
    12001 atacatactg ttttattctg atagacaagg taatgtcctg gcataagctt ctttactcca
    12061 cggaagaatg tattttcacc tgctacatat gtgaaagaca tataaatctg taacatgtct
    12121 tcatttaact ctttcacaaa tcctggttgc tccataatct tacgaataga tgaaccatag
    12181 agaagcttcc cattttcagt ctcataataa tagaaaggtt ttactccaaa ctggtctctt
    12241 aggcagaaga gtttcttttc cacagtatcc cataatgcaa aggcaaacat tccatgcatg
    12301 tgatttgcca tatcatatcc ccatgtctta tatgcttcca ctaaaattgc tcgttcacgc
    12361 tcatctctac ataatgaaga atcgattcca agttcttcac agagaacttt ccagtttcga
    12421 atatgtccat tgtattctag aatttttatc atatatgtgt cttttcgtgt taaatacttt
    12481 tcttctaaat ttatcatatg tgtatccttt cgtattaaaa catttaactt ctaaatttat
    12541 taaatgttta tatatattta ttcacttata atcactgaaa aatcgacttt tttcaaagta
    12601 aattcttttt gaattagtac taatctgctc tttattaatg gcataaatat gattatcaag
    12661 tattatcagt gttataacat tgaatttttt attcttcata aatattgccc cctttatatt
    12721 accaactaaa tataatatat cttatgttac ttacatattc tttaagaaat tctgaagatt
    12781 ttataaagtt ttttgtatta taattaatta aaaataaaat tctagttata aaacatattt
    12841 tccattaaac aaaaaaatag ttaaaataaa ttcataacca cataaactta ttttaactat
    12901 ttgtatattt taattataaa tattaactta aatttttaac aggatacact cataaaataa
    12961 acataatgaa caacaaaata atatgtctca gtttctttat atactttacc ttctttcaat
    13021 tttctaccat gtagacgtat aagataatat tagtaattca ctctattgct taactcgtgc
    13081 gatatggaca accaagaaag ctatttcctc atttgttaac ttttttccat atttcttttc
    13141 taaaaaatct ctaactttta atgcaattaa catttctttt ggatttgacc ttttcatttg
    13201 attaaataat aaatcatcac cactgcttaa catactatct gaaaaaaatc tttccacaaa
    13261 aaattttata tgtgttataa atctcatata atgcatactc tttttatcta aggttctatt
    13321 caaagaaaaa acaaaaatat taattatttc tccaattact ttggcatact taatcgtatc
    13381 atatgaattt ccatctgaag ccatagcatt tgcaaaatgg aaagcaatgt tagctgcttc
    13441 ttcctctggt aattgaatgc ccaatctctc ctctaccata taaattgcaa ccattccaac
    13501 cttaaactca tttggataat aattttttat ttcccaaaat actcgatttg taatttttat
    13561 tcctttacgc atcctttcaa ttgcaaagtt aaaatggtct gctaatatta agtataagtt
    13621 tttattaaaa gatgagttca ataacttttc tgcctctact attatttcct gtgttatctt
    13681 taaaatgtcg acaggaatat catccattaa ttctaaaaat ttttttgatt ttgtattttc
    13741 aacagggaca aacatttgat tatcattgga accacaaata tattctcctg tttttttacc
    13801 atatccaatt cctttcccta gcaaaataaa ttcattatac tttgcatcat gaactaaaac
    13861 aacgctcgag ttcaaaactt tctttattat gtagttcatt ttatttgctc accttctatt
    13921 ggtcatttaa aatctatact attattttca attacttttt tataccaata aaaactatct
    13981 ttcttatatc gattatatgt cccctttcca taatcatcag catccacata aatatatcca
    14041 taacgttttg acatttgttt cgttgattca ctgaccaaat caatacaacc ccatgaagtg
    14101 tatccccaaa gttcaacgcc atcttcgata atcgcatcta acatacattt aaagtgttct
    14161 tttaaataat taattctata ttggtcatga acttttttat cttcagttaa aatatcttta
    14221 gctcccaatc cattttcaac tataaataaa ggctttctgt atctatcata taattcaatc
    14281 aaagatatac gtagacctat tgaatcaatt tgccaacccc attcactcat ttctaaataa
    14341 ggatttttta cagctaataa cgtattccca ggtgtaatat ctaatccagt tgtatcagct
    14401 gcctcacaag aagacatata gtaagagaat gaaataaaat ctactggata ctttttcata
    14461 atttcatcat catgttcttc ttttgctatt tgaatgttat tatttttaaa atacgaaagt
    14521 aaatatactg gatactcacc aaaaacctgc gtatcactat atgcataaat acttctcatt
    14581 ttttgttgag ttgccaaaac atcttctggt ctacatgtat atggataata cgttaatttt
    14641 gttaacatac accctacttt tgaatttgga ataatctcat ggcaaatttt tgttgcaagg
    14701 gcactagcta caaattgatg atgcatagct tgaaaaataa cttcctcaaa gtttttatcc
    14761 ttaaaacggt ctcgaactaa accaccagta gtgtacggat gacgaatcat agaatcaact
    14821 tcattaaatg taagccaata tttcactttg tttttatatc tttcacagat agtttctaca
    14881 tacctaacaa acatattgat aacttgtcgt gaataccatc catcatagtt taaaacgata
    14941 tttaatggtg gttcataatg tgacatagta acaagtggtt caattccata acttatcaac
    15001 tcatcaaata catcatcata aaactgtaaa ccttcttcat ttggctcttt atcatctcca
    15061 tttggaaaaa tacgagacca agcaatactc attcgatatg tcttaaatcc catttctgcc
    15121 attagagcaa tatcttcttt atagtgatga taaaaatcac taccatgacg ttttggataa
    15181 tttacttcat cttcaggatg tgctaatcct tcttctatat cttttgttgt gatttcatta
    15241 ttagatttat agtctttcac atctgcatca aagtgtgcct ttgctacatc tgatacagac
    15301 caccctttac cacctaaatt ccaaccacct tcaaattgat tagcagctgt agctcctccc
    15361 caaagaaatc cttctggaaa ctttctcata tttatattcc tccctttaca tcaaacaaga
    15421 tacttccttt ttttacattg ccataagact ggtttaacac tttataagca taatctgctc
    15481 cattattgtt tacaatcata acagttgaat ctaaattagc atgtgagatt ttgtctaaat
    15541 caaattgaat taataaatct ccttctttta cattatcgcc tgtcttaaca aatgttttaa
    15601 atccatctcc attcatactt acagtattta ttccaatatg aatcaaaatc tcagccccat
    15661 tttttgttct taaaccaatt gcatgttttg tttcaaataa catcacaact tctgaatcac
    15721 aaggtgcgta taaatttcca ttggtcggta taattgcaac tccatctccc aaagtcttac
    15781 ttgcaaatag ttcatcatta accttactta aatctattac ttttccttct actggagtta
    15841 ctaagatatt tacttctttt tcttcttcta gctcttcttc aacaacatct ccaattgatg
    15901 actctactgt atttgtatct tccttccaaa taatcagtgt aataataaac gagcctaata
    15961 ttgcaataac aaaaccaata ctcgcaaaaa tcaaattgtt tccattgttt gggtcaataa
    16021 acatactcat actggcaagc cctggtccaa caattacaaa tccttttact gcaaataatc
    16081 ctaaaaatgt accagaaatt gcacttgaga atacaacacc taacattgct cttttatgtt
    16141 gtaaagtaat accatataaa gctggctctg taattcccat caaggcagaa attcctgcag
    16201 ataatgctgt ttgttttaat gtttcatctt ttgttcgcaa ggctactgca aaacaagcac
    16261 cactttcaga aatattgtgg gctaatgaag atgtcatata taaagcttca taacccaact
    16321 ttccaattgt attaattgaa taaggtatta gtgctttgtg cattcctgtt gcaaccataa
    16381 atggaagaaa agcagcaagt aatcctacaa ctaagaatcc cattttgtta tataagaata
    16441 aaataatggt tgtaaaaatt gtaccaacgt tatatcctaa aggacctaaa atagttagag
    16501 taattggaac agtaattgcc aaagacatca taggaacaaa gaaagttcga attggttttg
    16561 gtgaatactt gttaaaaatt ttctccattg ttgccaaaaa agacacacat aagatagccg
    16621 ggaaaacttg tgcattatag gcaatgtttg gaattgtaaa tccaaatagt tcgacacctt
    16681 cacttaaagc agttgttgtt gctggaagca ataagtatcc tacagcggca atagctacta
    16741 aacgattaca tttcaataca ttggcagtag ttacagctac cataagaggt aggaaataga
    16801 atgttgcatc tgaaatagca actaataatg tatatatagt agaatctgat tttaaaatat
    16861 taaacattgc taataacatc aacagtgatt ttaaaatacc agctcctgca atagcaaaaa
    16921 tcagtggttg aaaaatatta ataataaatt ctataaatct tgttcctaag tttatttttt
    16981 cttcagtttt tacagtacta tcatttgtat ctaaagggca aacttttaaa atttcatcat
    17041 acacatcaac aacactattt ccaatgacaa cttgaaattg tgccccatta actatactca
    17101 taacaccttg tacttttttt aaagcatcct gattgacttt acttctatca atcaacttaa
    17161 agcgtagtct agttgaacag tgttctaaag ctaaaatatt ttttgaacca ccaacttgtt
    17221 caataatgtc aatggcagtt tttttataat tcatcttgta atctccttct ccataatttt
    17281 ttatgaatgg ccattcaaga tgcaaaaaag gcagaaccta tataaacacc ttgaaaaatc
    17341 aaagcaatat attcgatcct gcctgcatta ccagtaacat gtcttctatt tatactattt
    17401 tgtattatta tattaacatt tgcctaaaag aattgtcaat acttttttaa attttctgat
    17461 actaactaat agcaccttta taattcattt tttatgtcaa ttaaagtaac agtagccacc
    17521 caccaattag tatcaaaatt gaacctggta ttttttcttt tatattttct ttgaatataa
    17581 aatgagcaag tattaaagtt attatcatag atacttgaga aaatggctct gctatactaa
    17641 gttctagatt atttaaagca attaataatg ctaaataaga atatccatta atagcaccac
    17701 ttaaaattga aagccccttt ttattactaa aaatctcaca aattattttt tgtttctttt
    17761 taaatgctaa aaagataaat aaatttaaac ctacgaaaaa atacagtata gttgaatata
    17821 atataggaga tacttgtaca aaaaaagcct tatccaatac tcttcctact gactgcaaaa
    17881 atatgtataa aaacattaat ctacatggta aattgttaaa aattacagag acattcttta
    17941 aaaaagagct tacttttttt aatataaaca ctccaccaat cataattatg attcctgcaa
    18001 cttttgctaa acttaaacct tcacctaaaa atataactga taaaagcagt aaaaacaatg
    18061 aatttaaact atttattgga gtaacaagtg aaacttcccc agttgctaaa gacgttacat
    18121 acgctactga accaattgaa tagactaaac tacttatata acatggtatt aaaaacgacc
    18181 aatcactaac tctatccatt atgctaaatg gaagtaaaca tactccccct ataaagaaaa
    18241 atagaaatga tgaagctata tttttataaa tatctccttc ttcatcacct aataatctta
    18301 caactactct ttcaaaagct aatagcacta ctctaattaa taatgcaaaa taagataata
    18361 tcatttttga cctcgcttaa aaattaatcc gacttattgt tatattaact gtataagact
    18421 accatatagg taagtctttg ggatttttta atattttatg tttaaataca ggtacattaa
    18481 gtttttcacc tagtttacgg cattgctctt tataggtacc ttttaattta tcatgaaaaa
    18541 ctatacaatc aattccacct tcaatactat ttatacactt ttctagtttc tcaaatcccg
    18601 attcatcatc atacattcca cttacaatcc attgatttgg caatacatgt ccaattgata
    18661 tgttatattc tttatttttt ataatatctg agaaagattt ctcaaaatgt acccctccaa
    18721 cacataataa agtcttcaaa ggttcatcac aatcaaatac tctaattaat gattttgcca
    18781 atacctgtat tgctatacta ttattaaaac tatcactact acttcctatc tcaatatcca
    18841 acataggtac agaatattct gtaattaatt tagattcttc accatatgtt gttccagacc
    18901 agtgagttgc ttctgttaat gtagtaaatt catcaagtag attttcattt tttatatcct
    18961 caatagcatt gattagattt ttaaagtatc ttggatttga atttccaaat tctcctgttg
    19021 gaacatctcc aattgtatga gctgttagga tatgttctgg agcattttct ccctcatgcc
    19081 aatttacaac tcctgcaaaa tcataatctc tgaaatgtct atttaaaatt ggaagataat
    19141 atttataatt atgacttatt acatcttttg tttttacaaa atgaaataag ttattgtttt
    19201 tatccttaaa ttctaataca aaattatcat caataacaat atcagtttga tttaaatcca
    19261 ttatttcttc acaacacttt atcacattac tggctactgg gtctttcttt aaatcactac
    19321 aaaagaaaaa aactgctttt ttattattca tccataaatc acactcctat aacttaaata
    19381 taatatatct tcatacctaa aatcatctaa atatcactct aattccatta aattatttat
    19441 atataatata ctaccatact cattttagct atacctttgc ctgagtttct atatatatgt
    19501 gatacatcag ctttaaatct tatagaattt ccactagata caatgtaatc ttctccattt
    19561 attttaatgg ttacttcgcc actaaaaaca gttataaact cttgtgttcc tggaagttca
    19621 gcatttgaat ctaaatttcc accttcttct atttcaatca taaatatctc aaatcttctt
    19681 ttggagtcaa atggaaatat tggataactt atatactttc cattatcttc aataaaatgg
    19741 tctatatcac ttttttgaat caattctatg tcactttcct gcctctccat caatgatgta
    19801 aatgagattt ttagtccatt agttattttc ttcaaagtag atatagtagg atttacctca
    19861 cacttttcaa tttgagatag catactctta cttacaccac taagttgtgc aatcttgtct
    19921 aaactaagat gtttttcttt ccttatcttt tttaaatttt cagcgattat ttcatttaaa
    19981 ctttgcataa atacccccta atacatacta atataagata aattattttg attattaaat
    20041 aaacttagta atttaataga aactttttat ccattatact gtacattatg tttaatatac
    20101 attacttttt ttagaatgta aatagttttc tgaaattttg tgaaagtgaa caaataaaaa
    20161 actagtaacc tatattttta caaggctact agcttttatt ataataatat ttcattttaa
    20221 ctataaaact tagatgcacc tataagctgt ctcttagaaa caatcttagc taggtttgct
    20281 aatgaagaaa tttttatatc tttaaaagaa actaaaaata caaacttaaa atctgtaaat
    20341 tcttgattta tattgtatat atcaaaataa catctcaaat tatcaaataa gtaattatct
    20401 tgtaagtctg cttctatctc atcagtagca tcttttaaat acgctcttac atcttcaaat
    20461 aaaacactga aataagactc atctatttct tcacctaact cttccaatat ttcttctata
    20521 tctaaatcat ccatatcaaa agaatttcct gtcaactggc aatcaaatac caatatattt
    20581 tcttgattta tagtatttga tatatcttgt acatattcga agtctggacg taattctaaa
    20641 atgtctttta tttcactgct ctttacatgg atttttcctt ttctattttc aatttttaaa
    20701 tctatcatat gctccccctt tcaatctaca aatctttaaa taccttgttt tataatcttt
    20761 tcaatatcta tccaattact aactctaact atattttgat gtttaacatc tttattatag
    20821 ttagtatcca gaagtattac tttaacacca tcatcagcta gttgcactga gtttaaagga
    20881 ttatcttcta taaatatgtc acaactcagt tctcttgcct tttcaacttt ataatcactt
    20941 cctaataagt aaacatctat atctgaaaat ccttgtctcg atagccaatt tttagttaca
    21001 tcagttaaac actcatgtct agccgttaca aaagataaat tatgtccttt agataatgtg
    21061 ctaattacct ctgtagcacc ctctacaatc tttgcttcat catatgagtg tatatactta
    21121 ttattaaact ccgcatacac atctcttatt cccgaaccat acaattcttc ccaattatgt
    21181 gttatacact ctgcttcagt tatattttta ttaaagatat catttaggta tggaagaaaa
    21241 tgataagggc ttgttattgt tccatctata tcaatacata tattcaattt gttcattttt
    21301 acctccatgt aacatttatt gatttgatta atcaaatatt atagcacagc tttaattata
    21361 aatcaaaact ctggatatga ctatacctat ataataccaa aaagcttgca tatttaagca
    21421 agcttttcgt aaattttaca tttttaacta atatataagt ttttctaagg ttggttccac
    21481 tccaaattgt gatttcagtt ctttatattc ttttaatgct ttgtcattat tcgttttagt
    21541 ttttatcgct cttatcatta aattttttgg tgtatcaagt ggagatatgt attctactac
    21601 agaagtatca tatccattgc cctccaataa caatgttctt aaaccatcag ttatcaaatc
    21661 tgcaaatcta gctttaaata caccatgttt taatattggt tccatagctt catatctata
    21721 ttgccctaat aactccttat gacaacatgg tactactact atagcttcag attttgctct
    21781 aattcctaga cctattgcca tatctgtagc tgtatcacaa gcatgtagac ttattacaag
    21841 gtcaacatcc ctattaggtg tataattagt caaatcctct tttataaatg acatattttt
    21901 ataacccaag tttttagcca tattttttga tgcttctata actacatctg aataatctat
    21961 tcctataaaa taacagtttt tcttcaatac ttcttttata taaaaattta agacaaagct
    22021 taagtaagat tttccacaag cacaatccaa gatagttata cagtctttat cctttatttc
    22081 ttttaaaata ctatctatca attcaacaaa atggtctatc tgattgtatt ttcttatttt
    22141 atcatttttt atcttaccat cttttgtaag tactccaatt tcttttaaaa gtgcatttgc
    22201 ttgtcctact tttacataat aatctctgtt ttttatttga gatgccgtaa attcatctat
    22261 tttaggtact tcttctttat tatctaaata ttttacattt accttcttat tgtctccttc
    22321 aattacaaca atagttcctc gctcaacata tttaaatact aatccatcat aattttctgc
    22381 ctctttagaa atattcttaa gcaactcatt aaacgtatat aaatctgatt ttcctttaaa
    22441 attaaattta tatttattat cttcctctat tgcaattcct ttaaactctt ttcttccaga
    22501 tttaaaaatc acctctaact caataaaaaa atgcctatta tcaaggtttc tttgagtaat
    22561 ccccataaaa aacatattta gttttcctat actctgttta ttcatcataa tctccctttt
    22621 aacttatatc tttaaatctt attaattaat attatttgtt tctgaattat ttatttcttc
    22681 ttttaaatcg ttcaatctat ttttaaaact ttctatctgc ttatctgata aatcaagata
    22741 ttctgttatc ttccctatat atttagggct tttagtattt attaatttca aagcactttc
    22801 catagatttt tctctaagct catatgcttt ctttacgtca tctctagcat tactcaatgt
    22861 ttctgtatac ttctccttag aaagttctgg aatctgcata ttttcatatt cttttacaac
    22921 ttctttacac ttgtttctgg catttgtaac atcttttttt atactagata atttagttat
    22981 attttttgct atatccttag tgtaaggtat acattctctc attggaacta aagctgctct
    23041 ttctatatta taaatttctt tcttaaaaat tcctatactt tccatatcta ttttttttat
    23101 atcttcacta tttttttctt tacttgtata tttattattt tcttttgtat tatcttcttg
    23161 tttttctgat ttattattca ctgaaacatt actatcagaa ttattctttt cttttatatc
    23221 attacttcct tcttcattac tttctttatt ttgtatattt ctatcttgtt tgttgtttaa
    23281 atttttatta tttgatattt cttcattctt tttattatcc tgttttaaat cggtactttc
    23341 tttttcctta aaatttgata catacttttc atcatatgtc tcatcagaaa tataattgtg
    23401 ataatacata gactctttta ttttattaag tccactcata taaatattac ttatcattat
    23461 tgttgtagct aataccacat aaatactact gtagatacaa gacctcttaa aattcttgta
    23521 atttctgaat atcactacta aaggaataag atataaaaat ataaaaacta atatccacct
    23581 cataagtcgt gtctcctttc tcaaaaccga aatccctcaa atatttattt ttaatcatgt
    23641 atcttatagt atttaccaat ttatgttttt taatcgtgaa cttttattaa attaaattct
    23701 gtatagtcac aagatgctaa tgtatattct actccatttc gaatcccctt caatcccatt
    23761 ttaaaagatt ctttaccatg caagtgacca taaatcacct tttcaacatt atattcttca
    23821 aataactttg taaataaaga ttcttccaat ttatcattcg taggaggata atgagtaatc
    23881 acaattattt ttgagtgacc acttttacga gcagattcta atgatagtct aagtctatgc
    23941 tcttctcttt tataaacttt ttcatcagtc tcatcaaatt taacatcatt aggacatatc
    24001 cagcccctac ctccacaaat tgcataatct ttatattcat aaaaatttgt ctgaataaaa
    24061 cgcatgtctt catatagctt atttaaactt gtaacggttg tccaccagta atcatgattt
    24121 ccttttataa gtattttctg acctggaagt ttgcttatta tatctaaatc ttttttagct
    24181 tccgataaat taattcccca agatgtatct cctagtacta agaccatatc ttcttcctta
    24241 acaacttctt tccaattatc tataattttc ttttcatgac catcccaatt acttccaaag
    24301 atattcattg gcttattgac tgatgttgaa aaatgtaaat cacctatagc atacaaactc
    24361 ataatatcat tcctttctta attacactaa ataggctgtc tctttttaga aattattttc
    24421 cattaagagg cagccccatt aatatttagc aaattttcta caagtagttt tttactgaac
    24481 tactttatca tctaccttag tatccttata ttgctctagt aatatatcta tttcttgttt
    24541 taattccaaa atttcagtta tatttttatc tgtttcagtt ctatcaagtt ttctttggta
    24601 ctgcatagct ttatgtgtat cacccaagtc atataactct tgtattctat ttagagtatc
    24661 tctaatagct atagacttca tttggtacat agcctgagct ttcattatgt cctctcttat
    24721 ttcactcatt tcttggtcta aaagaaaggc agcatcagat gctcttctaa gcctttcagc
    24781 aagagtctca ggtatgtaat ggtcatattt atattttaat gaatgttcta ttgtagccca
    24841 gaaattcatt gcaagagttc ttatttgtat ttcacatata atttcctttg aacccgcaat
    24901 ggagtttata gggtatttta ttataacatg atagcttcta taaccactat ctttgtaatt
    24961 tgtaatataa tctttttctc ctattatagt catatcattt cttactttta tcaaatcaac
    25021 tatagcatat atatcctcaa caaactggca cattattctt ataccagcaa tatcatcaat
    25081 ttcagtttct atatcttttg catttatcct ttttaactta gatattatgg aagatatttt
    25141 ttttgttctg cctgtgacaa actctatggg agaatattca ccttttgcta aatattcttt
    25201 tcttatattt ttaaatttta ttttcaattc ctctactgca tgttcataag gtgctagtat
    25261 ctcattccat ttttcgtatt ccatattgct caccctttat ttgtttttta tgaccttctt
    25321 tattttatca taatttattt tgtttttgta tagtaaagca attctccatt ttccaatata
    25381 taccctattt cttctaaatc aactaagtaa cttatgacag atactaacga agatttaaaa
    25441 aaatgatact ctttataatt attgcttaaa ttattgttat ttataatgtt ttttaataaa
    25501 atttctaaag atttcggttc tacaagcaac tctcttattt gatataaata tttttttaaa
    25561 gcattcaaat gtttttctat taatttataa gaatcttctt tcgatataac ttgcttgcca
    25621 tgccctaaaa ctaagtactc aaaatctata ttttttaatt tttctaatga atttatttgt
    25681 tcttgtatat catatagaaa caagaaatcg tattttgata gaatttcatc tcctatcaat
    25741 aaatcgccaa caaaaatcac attatccttc gtcaaaacag ctatactacc aggtgtatga
    25801 ccctgaaact caattatatc aaacatttca ttgtttaatt caatttttcc aacttctata
    25861 gatttatcta tctttatatc atccaacatt ttatttttta agatattatc cataaataca
    25921 ttgctctttc ccccaacaat atactttgaa aacaattctg gattttctat atataatttg
    25981 tcgtattcag atgacattat acacacatct ttataatatt ctttaaattg attacaagct
    26041 ccatagtggt catcatgacc atgagtattt ataatatatc taacctgtat attattttca
    26101 tctaatactt tatttactct cctaggtctt gaaccacata accctgggtc tatcataagt
    26161 gctgtgttat cttcaaatac atatattcct gtattcgtgc caccttttat aaaaaaggta
    26221 tttcctttta cttttgtaag attcaccttc ctcacctcat tttgacaata tttatgatac
    26281 aatttattac ttactaatat aaaactatac aagaacaatt ctttttattc tatctatagt
    26341 tctttttctt attttgtatt ctttagatat ccttattttc acattatatg ctaaatttct
    26401 ataaagtaat gactctataa aatattcaac tatacctcta tccctaaagt ctatatctat
    26461 atttccactt gaataaatat ttgagtctaa tatcataagc tcatcaaaaa agcttaatct
    26521 cttagattta cttaaataat ctttatttgg aataaatcta tcacaataat ctatcctgta
    26581 atttactact tcatcattta tttcaaatcc atattgcttt ttcattaact ctctatagtt
    26641 attatttttc tcaataacat tatctaaatt tttattataa tcctcttttt tccaatgtcc
    26701 aaacatccta caatttaatg gtctaacttc atatattaga catctattat tagtatcttt
    26761 aaatggacat gggttctttc ttgtatactc ctcaaaataa taatctaata ttttacttat
    26821 acagcttttt tttaaatcaa ctctatcttc aagataacaa aatatattca aaaactcaat
    26881 taaatttata cctactgact ccatacaaca atttccacat ccagaacaat ttcctactgg
    26941 caaggactta tatacatcat ttagtttgtc aaagagctta tttttaatgg aataatctat
    27001 acaatttaaa atatctttat tttttatact agtcattatt attactcctt gttataatta
    27061 ttaagttaat acaaacaatt attataacat attttttaaa tttaatcgca tatggaggta
    27121 ttttatgaat agatatacaa aaataattaa tatgatggga agttattaca caaaagactt
    27181 tgaaaaagaa aaaaagaatg taataaaagt tagagaagtt aaagaagata cagttagaaa
    27241 atttttcttg caaggtgact gtgaagttct tgtagtattt gaagacactg ggaaagaaat
    27301 acttattgat gacttttctc ctgaagaaga tatcaaaaaa tatttaggaa caaaatttat
    27361 aaataaaaaa agatagttat caaaaataat tctaaaatta aaatttaata aatgtacagt
    27421 tataattcta tataattata gttaaaagtt atcttttatt gcttttttag actttaagat
    27481 agcttttttt ctattttttg taaaataatt ccataattca cattaataat tcacaaaatg
    27541 atataatata acaaatagat aaattttaaa tttctaaata cataattaat attttgcgag
    27601 gtgattttat gcaaatttat tttagagtta aggctgttgg caaacgcaaa cctatgttag
    27661 aacttacccc ttttgatgta tctgacaaca tcaattgcct aaaagaagct atctctgaaa
    27721 ttgtatctaa aaatgttctt gaatataatg agaaaatccc agaaaaatca ataataaact
    27781 tcttaactaa tgaagaaatt gaatctcaag ctgatattgg aaaagttgga tttggctcta
    27841 tttacaatga gaataaacaa tctgtagaaa aggcaataga agtagcgtta caggcatttg
    27901 aagatggaat ctacaaagtg cttataaatg aagatgttat tgaaaaactc gattccccta
    27961 taaatctgaa aagtggagat atatttactt ttataagact taccttgctt gctggaagaa
    28021 tgttttaaaa taaataattt ggaggtacat aaatgttaga acgtactaga gtagaaaaaa
    28081 atcctaaaat aattgaaaat tttaaaaata attttactaa atcttcttca aaacagtact
    28141 acgacttagc aaaatctctt tgggactgca tagaaaatag ttcatactat tatagagaac
    28201 ctgaggacaa attcttaaat atgataaaga aatactacaa ttgccactac tacgaaatta
    28261 aaaatatgga cgagctcctt tctggacctt taaaaaatgc attagaatta atatttactt
    28321 ctgaacaagt agaagaactt tgccaattaa ttaaaagaaa gatggattat acatattcag
    28381 gaggatatta tcgtaaaggg tatcgctcaa ataaacttgt ggattatttt agaggtatta
    28441 taaggtttat ttcaaactat gttgatttaa tttattatga tttttcagtt atagattatc
    28501 ttactcttcc aaataaggtc ttgtactatg actctataat gagtgataaa atagcactag
    28561 aaatagacaa aggaaatcaa aaagtaacaa gtgccttaga agaagtaata tttggagaaa
    28621 atcaaactgc tctacttaca agacaaatga taattggtat catgaagagt catagtgata
    28681 aatgctatga aatgctttct aaacttcttt tggcagccaa acaacaagaa ggacttagac
    28741 agcaaattgt agagagttta gatgagggta caatttctgc atcaatatac atgttaaaaa
    28801 ctattttgga caacaattta gagcgattta gttctgttat aagagccttg gatacatgga
    28861 caggacttgg tttttcagac caaaaacctg gaataatacg taaatgttta gaactttcat
    28921 ataaatcatt gactgatgaa aagttccgta tagaatcaat taatagcaat gataatcttg
    28981 aaatttattt tgccttatgg gctactgcaa cttacaacat atatgactct tactctctta
    29041 ttgaaaactt actttcttgt caggaaaaat ataaaaaact tgtagctctt tactttttat
    29101 cgcatacaga aagtacatat tttaaacatt ctatagcatc taaaaacatt aagggtgatg
    29161 atttagagat actagcatgg gtaattacaa atttctatgt agaaggttct gttggctact
    29221 actatagttg gcaaaacaga gaaactaatt ttgacttgtc aaacactcct attgacaaag
    29281 actttgatgc tagaaaagaa caatttgaaa gtttaaaaca tactttagaa attttaaatt
    29341 caaagacaaa atcattttca ggaagtgtat ttccatggac ttggattgaa atttcttctc
    29401 atgaaatact taaatgtatg atgactttaa cagcatacga tatgaatgct caatttatag
    29461 atgtgcttat ggaaaattgt catcttatga gttcagattt aagagcttca cttatagtca
    29521 attttgtaaa tgaaccaaag acagatagac aacgtgaatt tttattggaa tgtttaagtg
    29581 ataagagtgt ttccaataga gaaacggtat tgagtttact tagaaagctt gagctaacag
    29641 atgtagaatt agataagata accaatctat tgtctttaaa gacaagctct attagacaaa
    29701 cagtaataaa aattttactt gaacaagatg attcaaattt ggaaaatatt gcttcttcac
    29761 tgataaaatc ttcaaattct caaaaatgcc ttggaggtct tgaaattgta aacttattaa
    29821 aagacaattc tgatagacaa gaactatata ataagcttat tttacttata gaggatattg
    29881 ataaatctaa aaagaataca tctcaagttc aaatattaat tgataatttg ttaggtaaag
    29941 caaaaaatga ttactgcaaa gaaaatggtt ttggaatata taaccctgaa aacaaagttc
    30001 aaattccaga acaatttgat tttaaagatg attctaatag ctcatcaaag cttaataaaa
    30061 ttatttcaaa tataagttta tctactagtg aggattctgt ttctgtagca acaaaaaaag
    30121 aagcaaaaaa aattctcgat acggatataa ataaaataat aaaagtttat gaaaaatttg
    30181 atgagcttat tactgaaaat gcagattatg aatatgaagt tgaaagatgg tcaggtaatg
    30241 tagacaaagt gcttttaggt tattctactt ataacttgac tcctattaaa tataaagttt
    30301 ctaatgatac tcttgataat tatccattgg cagaactttg gagagaaact agtaaaaagc
    30361 ttgacttaga ttacaaatta atacttgata tcttatttta tagtataagt acatgtggat
    30421 ataatgaaac agaaaaaagt tggttttcat ctatatattc aaatgtcctt gactctaaaa
    30481 aacaaaataa aatatataca aaacttgaaa aatttaaatt taatggactt acaagaaaac
    30541 ttttaagagt tttactaaat gagtttgacc ataaagaaat atttaactat tgtttagaaa
    30601 tatactataa acttagaaac ttattgacta tagaacaatg taaaaaacaa agagttgaat
    30661 ttactaaaaa ctattacaga caaaatgaat actgccctgc tttaaatgcc cctattttaa
    30721 actattggct tgataaagca cattcgttta tttatgatga tatttctttt actaagtttt
    30781 tctacactac gttttcaaat tatcagttgc tagaatataa gtttcctttc tatactagca
    30841 taaatgtgtt tgctcgtgca catgaattag gtttaatgac tgaagagcaa gttaatagag
    30901 aaatgataag tggatacaac gcatcaaata taataagaac attaacaaat cctagatggg
    30961 ctggtgacac attagaaaag tacccttggc ttaaagataa tttaaaaact gttacaaacc
    31021 gaattataga aattgaatca cgtagaggtg atatgccaac agaagtgtct catctagcta
    31081 ataatataca atactttgaa ggtatagatt attttgtaaa tattttggtc gctcttggta
    31141 aagaaacttt ccagagagga tattcatatg gtagcaatta ttcaaaaaaa gatgttttaa
    31201 gttctctact taaaaattgc tatcctacta aagaagatga ttcaaaaaaa ttaaaaagtt
    31261 tacttaaaaa taccgacatt actgagttaa gattaataga atctgctatg tatgcccctc
    31321 aatgggtaaa cataattgaa aaatatttaa actggaaggg tctgaaaagt acaatttggt
    31381 acttccatgc acatataaat gaaggcttct ctgcacaaaa agaatcagag gttgctctat
    31441 tctcccctat ttcaccacgt agtttcattg atggtgcatt tgatgtagat tggttcttag
    31501 attcatataa aactattggg gaagaaagat tcaatatact ttacaagtca gctaaatata
    31561 taactagtgg tggtaactct catagacgtg cacaactcta tgctgatgct acacttggta
    31621 agcttgataa agacatgttg atgaaagaaa ttgaaacaaa tagaaatcaa gataaattga
    31681 gaagtttctc tttacttcct atgaaaaaag gagacctaaa agaagcaact ttccgttatg
    31741 aatttattca aaagtttgct aaagaaagca agcaatttgg tgcacaacgt cgtgaaagtg
    31801 aatcaaaagc ttgtactata gcgctagaaa atctagctag aagtgctgga tttggagatg
    31861 taaatcgtat gatgtgggct cttgagactg aaaaactaaa tgaagttgct catctattta
    31921 aaccaaaaga tattgatggt acttcaattt ggatagatgt tgatgaaagc ggaaaagcat
    31981 ctactaaagt tcaaaaaggc aacaagtctt taaagagtat accaaaagct attgctaaac
    32041 atccatatgt acttgaactt aaatctgtcc aaaaagattt aaagagtcag tattctcgtg
    32101 caaaacagag tttagaacaa gctatgaaaa atgaaatagt atttacagtt aaagaaattg
    32161 ccaatatcta taaaaatcca gttatctgcc ctttgattaa aaatctagta tgggtatgtg
    32221 atgataatat tggatatgtt tataatgata agtctaagtt ctttatttgc aatatagaag
    32281 gtgaaactta taagttgaca gcaaaaaata aattaaaact tgcacatcca gttcatttgt
    32341 ttgaatctgg taagtggagt gagttccaac atgatttatt tagtaagcaa cttgttcaac
    32401 catttaagca agtattccgt gagtattata gaataaattc tgatgaaata tctgaaaaaa
    32461 ctatatctcg cagatatgct ggtcaccaaa ttcagccaaa gaaaactgta gcacttctta
    32521 gaaatcagct ttggactgtc gattatgaag agggattaca gaaagtattt tataaagaaa
    32581 atatagtggc aaagatgttt gcacttgcag attggttttc accagcagat atagagcctc
    32641 ctactattga aacagtagaa ttttttgata gaaaaactta taaaagttta gaattagctt
    32701 ctatacctcc aattctattc tcagaagtta tgcgtgatat agaccttgtt gtaagtgtcg
    32761 ctcatgctgg tggtgttgac cctgaaacta gccattctac tgtagagatg cgtatagcaa
    32821 ttgctaaaga acttttatta ttattaaaaa ttaacaatat caattttgaa ggctctcatg
    32881 ttcttatcac aggtaattta ggtgagtatt ctgtgcatat gggttctggt attgtacata
    32941 aaaaagggat gggtgctttg aatatacttc ctgtacattc tcaaagtaga ggtaagatat
    33001 tccttccatt cgctgatgaa gaccctaaga ctgctgagat tatgtctaaa atcttattac
    33061 ttgctgaaga taataagatt aaagacccta gtatcttaag caacttataa atataagatt
    33121 tatctctaaa acatattggg ctttctaatt ttccatgaaa gtccaatatg ttttatatta
    33181 tattttatac gaattaattt ataactatag caaatttata tgatatcttc atcatacata
    33241 ttgtatatca ctgaaaattt ttttcttcat taaaaccttt taaccctata aaatctgtat
    33301 agaaaatctt tttctaaaga tttatttaaa gtagcacttt cttgaaaatt tataaatttt
    33361 atctctaaag aagacttatt tataggtata accttatgta taagtcttaa atttactatg
    33421 tattgtctat gagtcataaa gaaattagga ggtaggcgtt tttttaaatc atcaaagttc
    33481 tcataaaatt ttatcgtatc ttcatctcta gtatataaat taatagctct tgaatttttc
    33541 tcaaacatga ctatatcatc aaaattaatc atatgtatct gtttccttat tttatataca
    33601 aaaagagtat cttctataaa attatttttt gcaattttat gagagttaat gtggtctatt
    33661 gctatattaa ttgaatcaag tattttttct tttttaaacg gcttaactat aaaatctata
    33721 ggatgtactt taaagctctc aagactatag tcagcaaaac ctgttacaaa aactatatgt
    33781 atatctttat ctatagaaag aatttctttg gcagaatcaa tcccattttt atatggcata
    33841 tcaatgtcaa taatcaataa gtctattttc tcacttttta taaattcaat tgcttctata
    33901 ccatccttga ctttcttaac ataatctaca tatccggact ctttaactat atttgctata
    33961 tcatctctta caattttatc atcatcacaa atcaaaactt ttatcacaga cttctctcct
    34021 cctaactaat aatattttgg tattaaaatt acaaattcag ttaaaaaatc gtcactattt
    34081 acaagtatac tataatcata cttatctagt atatttttaa ctagatataa cccaaaacct
    34141 ctgtcttgcc cttctttagt gctaaatcca tccaaaaata tactttcgac attatgtatt
    34201 ttaggaccat tattttttat ggctataata tactgaaatt catcttcata tgcatgtaat
    34261 cttatatact tcttttcctt ttgtatagca gacaaagatt ctattgcatt gtctattatg
    34321 tttaatagca ctgaactcat atcattaggt tcaatctcta aattatccat aaaatcttgg
    34381 atatatacat caaaagatat gttcttttca agacattcct catattttaa ggaagctata
    34441 gcatctaaat aagatacttt cccatatctt gaattttttg atttaaagct atttgatata
    34501 ttcgttatat attcttttgc atcttcagct aacccagtat ttaatagaga atatactact
    34561 tgtaaatgtt ttaaatagtc atgtttttga cttcttaatt ttacgattat attgtcctta
    34621 tgatttattt tactgtctaa tttttgtagc ttatactctt gtttattgtt attgtctagt
    34681 aatcttataa atttaaatcc tatataaaag cttaaaataa ttaaaattaa aaaaagtata
    34741 atatttaata ctccttcaag taaaaaatta tatcctaaac ttaattgtat atactcaata
    34801 ccctccatga ataaaatcca tacaaaacac gatacaaaaa acgttaatat tcctcttttt
    34861 aatatgtcta attctttata aaacctgtat ttattcatta taagattact aaatataaac
    34921 aaagttagta ttactatagg aaatataaaa ttattctcca taattcccct cccatctaca
    34981 ctttatatga ggattatatc attttagaaa aattatgtaa aaagtcaaaa tttacatgta
    35041 cattaattaa acttgcaaat acacatataa atttgtacaa ttaagtactc aaaacttgaa
    35101 gatacttact ttgatatata ttttaaataa acatataatt gatttagcta agaaaggaag
    35161 tggtaaatat gaaagataat ttaaaaatag cactaagata cataataagt tataaagcaa
    35221 ggtctttagc aattgctcta agtataattc ttgctacatc acttattgtt ggtattggta
    35281 ccctctcaag aagtgctcaa caagcagagg tagataaatt aaaaagagaa actggaagtg
    35341 atcatgtata ctttaaagat ataaataaaa atcaattaga atacataaag tctagaaaag
    35401 atattaaaaa tttaggtata acatctcact atggctatac agatcctaat gaaagattag
    35461 ctataaacct agaatatgca aataaaaatt acttgactaa tcaatccaaa cttataaaag
    35521 gtcaccttcc aaaagctagt aatgaggtgg tcgtagagaa atggatttta aatagtctgg
    35581 gtttaaaacc agaaataaat cagaacatta cttttaaatt atatcaaaaa gaaaaaccag
    35641 agacatttaa agttgtagga atacttgaag atagacctat tgagaaaaat aaagggacat
    35701 gtgaaatttt tttaaatctc aatgaatcaa aattggataa attttcttat gcttatattg
    35761 aatttaataa tggcattgat ataaatacaa gcatagataa tatagtaaaa aatactatgt
    35821 tggatgagaa tagtgttgga aaaaacaaaa tgcttataga atctactagg gaaaatggta
    35881 ctttagataa ctcaagtaaa tacactgcaa ttactatgag cctattttct ggtattgtaa
    35941 tatatagtat ctatgttata tcgatatatc aaagaataca ggaatatggt atcttaagag
    36001 ctattggatc cactaacttc aagattttca agtttatgtt ttatgaactt ttcatacttg
    36061 ctttgatagc aatacctatc ggtatatgca caggtattgg tggtgcacaa atatttaata
    36121 ggactgcagg aaatattcaa tttgaaggta atgtaacagt tacccctttt gttattccag
    36181 ataaaataat acttttatca attggaagta taatactgac aatactaatt ataagtttct
    36241 ttacatattt aaagataaga agaatttctc ctattgactc tataagaaaa acctttggaa
    36301 ctgataaaaa tataaataag gtcaattctt tgatatcaaa attaacttta aatatatcag
    36361 taacaaaatc tatatctgct aaaaatatct ttaggaataa aaagggcttc attataatta
    36421 tattatccat gagtctagga ggtataatga tcataaagga gaattataaa tattcttttt
    36481 cagatataca aaataaaaat ggtcaagaag aaacatatat gaatgcagat tttattctaa
    36541 caaatttttt cttaaaacta aatcaatcta ataaagctga ttctttcaag gatataaaag
    36601 gacttaatga taatcagata gataaaataa aaaatataaa tggaattgac aaagtcaaaa
    36661 ctgccagcat cttagataca agaatagaag tagataaatt aaatggatta gattactaca
    36721 atattattaa ttcaacgcct tattataagg attttcctct attcataaaa aataagaata
    36781 ctggaaaata tacaatgaaa caaaagttaa gaggttacaa tgatgagatg ataaattcat
    36841 tagaaaaata cttagtgagt gggagtataa atctggaaag aatgaaaaaa gaaaacctcg
    36901 ctatattata cgtaccacaa gtttctaaaa caaataaata caagctaagt tatactcctg
    36961 gtacagaaac gcctgctgta gatataaaag taggagatac tataaaggtc aaatatccaa
    37021 aaggtgagat agatcaagat ctttatataa aactaaaaga taattatgaa tatctagaat
    37081 atgagtttaa agttggagct attgtaagtt atccttttgc agataattac ctatattctg
    37141 gagatcaggg tatagatgta ataacaagta acgactatct aaaaaaacta actggtgtag
    37201 ataaatataa tgtagtttat gtagatatta ataaaaatgc aaatgtaaaa aagataaata
    37261 cactcttagg tgaaatagga agtgaatctc ctggaactac aacagctaat atgctaatgg
    37321 aaaaagaaaa ctttgataag atgacagcta gggctctgac ttatgcttat ggaatagtag
    37381 cagtaatgtt tataattagt gtgtttaata taataaataa cataagttat aacttaactt
    37441 caagaactag cgaatttgga atgttaagag ctattggtat ctctgagaga ggatttaaaa
    37501 atatgatact ctatgaggga atattatatg gaatactctc aagtgtaatt accattgtgg
    37561 tagggcttat aatacagttt agaatgtatt acacgtataa ttttgtatca tatggattgg
    37621 gattttcaat agattacaaa atatatatcc tagttgtttt agcaaacata attgtaggta
    37681 ttctagctac atatattcca ttaaggaaaa taaataaaat tagtatagtt gaagctataa
    37741 acattacaga ataggaggat taattatgcc tatattagag attcttaact taggtaagat
    37801 ttatggaaaa aaggaaacag ctgtacatgc actaaaggat gcaaatttaa aaataaataa
    37861 gggtgaattt gttgcgataa ttggcccaag tggaagtgga aaaagcacat ttctacattt
    37921 agtaggaggt cttgagagac caagtagtgg aactataaaa gtcgatggta aagatatatg
    37981 ttgcctttca gataaaaaat tagcaaaata tagaagacaa aaagtaggct ttgtttttca
    38041 acagtataac ctaattccag tattaaatgt aaaagaaaat atagaattac cactaaagtt
    38101 agacaataaa aaagttgatg aagaatacat cgaagacatt ataaatttat tgggtcttag
    38161 tgagaggaaa aatcatcttc caaatcaatt atctggtggt caacaacaaa gggttgctat
    38221 tgcacgcgct ttatctgcaa aaccaagtat aatcctagca gatgaaccaa caggaaattt
    38281 ggatagtaaa actacggaag aagtcatgga tttactaaaa agttctataa aaaaatataa
    38341 ccaaacactt ataataataa cacataatga aaatattgct agaaaagcag atagaattat
    38401 atctataata gatggagagc ttaaattaac actctaaaac tatttataga aaagaggatt
    38461 taaatgttat tgattttaga taatttaaat ttaaacttag attttgacta cagaataata
    38521 agagaagaaa atgatgatgt agatatattt atagatatta attatcgtag cttagatata
    38581 gatactgatg gttctaactt atttaattct agaatacaat ttccatttgt aagagcattg
    38641 attctaagga taaataagaa caatcaatgt atgacaattc atttgttaag agatatagat
    38701 ttattctctg cttttgccaa ctttgaagtg gattatactg actctataat aaatataaaa
    38761 aatcaaaatg agaaagtaat tttaaataaa tctatcaaaa aatagtaagg gggccttaat
    38821 aggccccaat ttttttcttt tctaaattta ttatatcatg ataaataaat tagtcaatag
    38881 aattttctga atatttttat a
//
LOCUS       DAGQWR010000017        38215 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.017, whole genome shotgun sequence.
ACCESSION   DAGQWR010000017 DAGQWR010000000
VERSION     DAGQWR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38215
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.017"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(39..2372)
                     /locus_tag="MIW95_000790"
     CDS             complement(39..2372)
                     /locus_tag="MIW95_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY3150138.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLL
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(2347..3063)
                     /locus_tag="MIW95_000791"
     CDS             complement(2347..3063)
                     /locus_tag="MIW95_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3150139.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMRLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVNIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAINTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            complement(3478..4134)
                     /locus_tag="MIW95_000792"
     CDS             complement(3478..4134)
                     /locus_tag="MIW95_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY3150140.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGASIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(4189..4818)
                     /locus_tag="MIW95_000793"
     CDS             complement(4189..4818)
                     /locus_tag="MIW95_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBY3150141.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(4949..5590)
                     /locus_tag="MIW95_000794"
     CDS             complement(4949..5590)
                     /locus_tag="MIW95_000794"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBY3150142.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTVSVTAI
                     KGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(5599..6270)
                     /locus_tag="MIW95_000795"
     CDS             complement(5599..6270)
                     /locus_tag="MIW95_000795"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBY3150143.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(6275..7159)
                     /gene="rsgA"
                     /locus_tag="MIW95_000796"
     CDS             complement(6275..7159)
                     /gene="rsgA"
                     /locus_tag="MIW95_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBY3150144.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(7257..7994)
                     /locus_tag="MIW95_000797"
     CDS             complement(7257..7994)
                     /locus_tag="MIW95_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBY3150145.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVENMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(8102..10117)
                     /gene="pknB"
                     /locus_tag="MIW95_000798"
     CDS             complement(8102..10117)
                     /gene="pknB"
                     /locus_tag="MIW95_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBY3150146.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     EEEEEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGSSNSGNSGGNSGNNGGNSGGSNSGNSGGSSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(10134..10886)
                     /locus_tag="MIW95_000799"
     CDS             complement(10134..10886)
                     /locus_tag="MIW95_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBY3150147.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(10906..11937)
                     /gene="rlmN"
                     /locus_tag="MIW95_000800"
     CDS             complement(10906..11937)
                     /gene="rlmN"
                     /locus_tag="MIW95_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBY3150148.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGNIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(11945..13270)
                     /gene="rsmB"
                     /locus_tag="MIW95_000801"
     CDS             complement(11945..13270)
                     /gene="rsmB"
                     /locus_tag="MIW95_000801"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBY3150149.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(13332..14033)
                     /locus_tag="MIW95_000802"
     CDS             complement(13332..14033)
                     /locus_tag="MIW95_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBY3150150.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(14102..14854)
                     /locus_tag="MIW95_000803"
     CDS             complement(14102..14854)
                     /locus_tag="MIW95_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBY3150151.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNVGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIDFLTGKNILICN"
     gene            complement(14864..15793)
                     /locus_tag="MIW95_000804"
     CDS             complement(14864..15793)
                     /locus_tag="MIW95_000804"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBY3150152.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTIYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(15808..16248)
                     /gene="def"
                     /locus_tag="MIW95_000805"
     CDS             complement(15808..16248)
                     /gene="def"
                     /locus_tag="MIW95_000805"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBY3150153.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(16266..18791)
                     /gene="priA"
                     /locus_tag="MIW95_000806"
     CDS             complement(16266..18791)
                     /gene="priA"
                     /locus_tag="MIW95_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBY3150154.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNEIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(19030..20229)
                     /gene="coaBC"
                     /locus_tag="MIW95_000807"
     CDS             complement(19030..20229)
                     /gene="coaBC"
                     /locus_tag="MIW95_000807"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBY3150155.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLAFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(20234..20500)
                     /locus_tag="MIW95_000808"
     CDS             complement(20234..20500)
                     /locus_tag="MIW95_000808"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBY3150156.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(20503..21120)
                     /gene="gmk"
                     /locus_tag="MIW95_000809"
     CDS             complement(20503..21120)
                     /gene="gmk"
                     /locus_tag="MIW95_000809"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBY3150157.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(21150..22031)
                     /locus_tag="MIW95_000810"
     CDS             complement(21150..22031)
                     /locus_tag="MIW95_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBY3150158.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(22035..22865)
                     /locus_tag="MIW95_000811"
     CDS             complement(22035..22865)
                     /locus_tag="MIW95_000811"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBY3150159.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGVLEV
                     "
     gene            complement(23152..24186)
                     /gene="ltaE"
                     /locus_tag="MIW95_000812"
     CDS             complement(23152..24186)
                     /gene="ltaE"
                     /locus_tag="MIW95_000812"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBY3150160.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            24482..26257
                     /locus_tag="MIW95_000813"
     CDS             24482..26257
                     /locus_tag="MIW95_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBY3150161.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEIANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIVLSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(26472..28124)
                     /locus_tag="MIW95_000814"
     CDS             complement(26472..28124)
                     /locus_tag="MIW95_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBY3150162.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEYLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGLLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLLEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGCFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYIISRNKKTPSRDKQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQITKLKNSF"
     gene            complement(28423..29634)
                     /locus_tag="MIW95_000815"
     CDS             complement(28423..29634)
                     /locus_tag="MIW95_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBY3150163.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLLLRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(29918..30451)
                     /gene="pyrR"
                     /locus_tag="MIW95_000816"
     CDS             complement(29918..30451)
                     /gene="pyrR"
                     /locus_tag="MIW95_000816"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBY3150164.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(30810..31757)
                     /locus_tag="MIW95_000817"
     CDS             complement(30810..31757)
                     /locus_tag="MIW95_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBY3150165.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKAKYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDFVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEAIKKIK"
     gene            complement(31726..32172)
                     /gene="lspA"
                     /locus_tag="MIW95_000818"
     CDS             complement(31726..32172)
                     /gene="lspA"
                     /locus_tag="MIW95_000818"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBY3150166.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(32482..33261)
                     /locus_tag="MIW95_000819"
     CDS             complement(32482..33261)
                     /locus_tag="MIW95_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY3150167.1"
                     /translation="MKKAIWGICICLLSIVGVYTYNHNTHNQNSSKETYTLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQILDILKKNNVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYGSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDTIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(33311..33793)
                     /locus_tag="MIW95_000820"
     CDS             complement(33311..33793)
                     /locus_tag="MIW95_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBY3150168.1"
                     /translation="MDTKKYKDKLLKEREKLTNLVVDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDSALSKIENGTYGFCDLCHNQI
                     DLERLDILPDTNLCNNCAKKENALLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGTLRD
                     "
     gene            complement(33977..35398)
                     /locus_tag="MIW95_000821"
     CDS             complement(33977..35398)
                     /locus_tag="MIW95_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBY3150169.1"
                     /translation="MVSFLGSIVVLIVGYFIYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGIVVAI
                     ILGLLFFKKAKDIKEQNAHKLAS"
     gene            complement(35544..36314)
                     /locus_tag="MIW95_000822"
     CDS             complement(35544..36314)
                     /locus_tag="MIW95_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3150170.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSSIEVVNEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYENYAVDAFSLDVFDYVLKP
                     YSEERIISMLNKLEKSEMSDIELSNSNSNLYKYKKETVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(36319..37521)
                     /locus_tag="MIW95_000823"
     CDS             complement(36319..37521)
                     /locus_tag="MIW95_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBY3150171.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIVGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRTDNGT
                     LIVFYVKE"
BASE COUNT        12205 a         6957 c         3805 g        15248 t
ORIGIN      
        1 ttattctata gactttataa ttattaaaga agtctaatct aatttttgtt aaaagtaatt
       61 gtaactttaa ataaatcacc atccacactt atattcatct taccaccctg taattctata
      121 aaactttttg ctattgctaa accaagacca gaaccttctg tgttacgtga tttatctcct
      181 cttacaaatc tctccattat atcatctaca ctaaaatcaa tctcagatgc agacatattt
      241 ttaagcataa tatttacttt attatctgtt tctaaaatat caatatacac tcttgaattt
      301 ggcattgcat atttgcttat atttattata agattttcaa atactctaaa tgtacgctga
      361 ctatctaatg gtaatattat tttttcatta gaaaagttat tctttactat tagagatgaa
      421 tctgcaagtt tgtcatctaa ttctaaaagt gtctgcttca ttagagatac aatatccaca
      481 tttactatat tcaaataaac atctccacta tttgccttgc ttacttcaaa caaatcttct
      541 attagatgtt gcaatctttg tgactttctg tctaatgtat caatatacaa cttacgattt
      601 tcatctgtta tactttcatc ttttaataaa tctatataag tgattataga tgttagaggt
      661 gtttttaagt catgtgatac atttgatata agctctgttt tcatcttttg actcttaacc
      721 tcttcgtcaa cagctttttt aaatccagtt tgaatatttc ctaattcgtc ttttatagga
      781 ttaaaaaatc ctaaatcttt ttccatattt acatctaagt ttccatctga aattttcttt
      841 gtaacatcta aaagttgatt atattcatat attgtctttt tagagtaatc tgacagtatc
      901 ttaaataata ctactgaata tattaatgcg agtattattc caaagaacca catactacac
      961 attataacta taatcaataa atttattccc aaaattgtaa ctatagtttt atttggcttt
     1021 tcttttagat ttattgttct aatttgatta ataactgttt ttcctgcttt ttttaaccat
     1081 acaaaaaaca ttcctgtcac agtatgtttt gtaaaatact ctcttattcc aacatttaaa
     1141 atatgtttta aaagtattat aaatacaaat attattgaga atattacaaa ccaataagtt
     1201 aaattcatta aacttaccaa tatgctagcc attttagcac ttatgtcatt gtttataagg
     1261 tactctacat aattcccact caaggtatta cttattataa cagtaggata taaatctaca
     1321 agcccctgta taatagcaag agcaataaag agtatctcta acggtattct taataatgta
     1381 tcaaatccta gcaactcttt agattttcta tatgggaatg ctaaagctac aataacaact
     1441 atagctaata taatttttaa aatcattcca gaaatcttat aataagaatt attttctaca
     1501 ttgcttatat atgaaattat accatcatca aaataagtat ttccataacc atactctaca
     1561 ctatcacctt tacttattac tttaggtaca gcatatacta atgttatatt ttttattgga
     1621 gataacttat atgaaattga atttcctcct aaacttgtat ccattaaatc ttctcttgct
     1681 ttagataaca acatttgatt aataactttt tgatttactc cataagaatc cacaatattt
     1741 acttttccat ctttatcaaa attcattaca acgtagaatc tatagttttc ctttatatct
     1801 gtaatctgct tttgtctttc ctcatcattc atttctgtat tttttgactg attttcatca
     1861 gaattttctt ttgaagcatc attctttgtt gtatcaagta ctcctaattc tttattatca
     1921 ttaaaaaatt caaaattgct ttctttatca actaaatagt actttaaatt ctttcctgac
     1981 gctttaatga aatgctccca attttcaaag ttttcattaa aatctgcatt aatactatca
     2041 tctgtataat aagtttctga accatcataa gtatcttttt ctttacttac attttttata
     2101 tagttcatag tttcttcatt gataaatagc tttgatggtt gtatcatctt accttcttgt
     2161 tcttccttca tattatgata taaaacataa cttcctttat atatactatc tacaaagctt
     2221 tcctgttcaa atggattctt tgctccacct ttagcacctg cttgtatagt agaatattga
     2281 ctaatcatac ctaatgatgc tgataacata agtataaaaa ctataatagt aactatatat
     2341 ttatctttaa attttttcaa ttttgtatcc aacaccccac accaccttta aatatttagg
     2401 attttttggg tcaatttcta tcttttccct tatattcctc acatgtacca taactgtatc
     2461 tgtatttata gcattttcat tccaaactct ttcatatatt tcatcagcag aaaaaactct
     2521 tcctgcattt ctcataagta aacttagtat tttaaattct ataggagttg tttttatata
     2581 ttttccatca atattaactt cttttgtatt ttcatttaat tctaatccgc ctattgcaaa
     2641 cacactatca tcactttcta ccttttgctc tttatttcca accatgttta gatacttagt
     2701 gtaccttcta agttgtgaat taactctagc caatagttct aatggcttaa aaggttttgt
     2761 tacataatcg tctgctccta tattaagccc cattattttg tcaacttctt ctgattttgc
     2821 agatagcatt attactggaa aattatagtt ttctctaagc ctcattgtaa gtgttatacc
     2881 atccatttta ggcatcatta tatcaactat agctaaatgt atttcttggt tttctataac
     2941 cttaagacct tctaatccat tgtatccctt aaaaacatta tatccttgat ttaatagata
     3001 tatctctata gcatctgcga tctccttttc atcttctacc accaaaatat tatatgaatt
     3061 cattttaatc tcctttatat taatttataa attttcttta catatatgat tatacaatat
     3121 gatagaagtt tttccattta tatatatatt tatatctttt gttctggtta tttgtattta
     3181 atgtccatct tagaaacctc cttctagttt aaacttaata ttttttcatt ttgttttata
     3241 tacttaatat taaaattaaa aacttaagat attacatata cttttctaaa gattttctaa
     3301 agatttaaat atatttagaa ttaatatata aaaaaaagta gttcttattg tattaattaa
     3361 atcaatgatt tgaaatataa aattaatatc aataaaacct actttttaat tttttaaata
     3421 tttattttta atttatcttg tttttaattt actttgattt taaaatgtcc ttgactttta
     3481 ttttataaat gtacatattt cttctactgg ttttttagat ggactcttca aattgtcctc
     3541 tggcactcca agagctaaca ttgcacctaa aaaatactct cccttttcaa aaccagtttc
     3601 tgcttctaaa acagcttcta tctcatctgc tgcatagttt tgacttgtca accaacaaga
     3661 accatatcca agttcaattg ctgatagtgt aaagttttca attgaagcac ctagactctg
     3721 catacctgga tttcttataa ataatttatc tatagtctcc ttaggtgcat ctataagctc
     3781 taattcataa tatcctgatg gattatatac ctttgtaaaa actaatacta aaactggagc
     3841 ttttaagtaa aatagtgtaa agtttttaac aaattttcta aatctattag ccttatccac
     3901 tgatacttta tccatttcca caagtatttc ttgttgcttt ttagttatca catctgctat
     3961 cttttccatt aaatcacggc gttttataac aacaaaatgc cagttttgaa tattcttgcc
     4021 tgatggtgct gcacctgctg cttttatcat ttttaaaata tcttcatctg atacatcttg
     4081 atttttaaac tttcttacac tttgtctttt aaaaatagta tcttgtaatt ccatttttaa
     4141 atccccccat aactttttta ttattttaat ataattttta aactcatctt attttaatat
     4201 atcatgttct aacaaatctt taaatgaatc tataaaataa tcatatacat ctacattccc
     4261 aaacccatat ttggcatata taaaaggtat acctacaaac ttagcactat ttgcatcacc
     4321 ttgcgtatca ccaacataga ttggatttat taatttattt ctttcaatta taagtttatt
     4381 attttctcct ttatgcaaac cagtattacc aggacattca aaatctataa aatactgttc
     4441 tagtttatgt gcttttaaaa aagattctat atatccagct tgacaattac ttactataaa
     4501 taatttatat tttttagata gtaatttaag agtatcttct atatcatcaa ataatttagc
     4561 tccattttca gataaatagt cacattcttt caaagaacat tcattcaata aattcattct
     4621 ttcttcttca cttaaatttg gaaataaacg atttgctatt tcctcaaaag aaagccccat
     4681 aagacttcta aataaatctt ttgtgacact taattctatt tccttatgtt tacttaaaac
     4741 atcctgccag actttgcata cttcttcagt tgaatcccac aatgttccat ctaaatcaaa
     4801 aataatacta tctaccacaa aaaatccccc ttatgtcaaa gtttattata tatttataat
     4861 tatatactaa aaagcagata caatcataaa tatattttac ttgattgtat ctgctctaca
     4921 atataaaacc aattgtcaaa atcaaatttt ataaatttct aactacaagt acacagcctt
     4981 gctctacttt aatcttacaa gaattactca acataacatt tgatattcca ataggtacag
     5041 agtatttcat ataataatta tcgagagggt attctaagcc agtaagtgtt acacccttag
     5101 catcaccttt tatagctgtt acagaaacag tgtcccctgg gtttccatac aatgaaattt
     5161 cctcattttc aactatatac atctcttctt tatcagaaag tatcctagaa tatataccat
     5221 cttcttttaa ataatataat agctttatat ttgcaagagt gtggtcaatc cttcccccta
     5281 aagcaccaaa aaaatcaata tgatttgctt taagtgtctt agctaaaaaa agacaaagct
     5341 ctgtatcagt ctcatctttt ttagatggaa atttttcaaa tttaacacct ttatttttat
     5401 agaagtttat cttttcttct tctactgaat ctaaatctcc taatatataa tctggcatta
     5461 tttccatttt ataagtgtga tttgcccctc catctgcaca tattatgcag tcataacatt
     5521 ccttaattat aatatctctt gtaacttcgt aatcttcaat ttcgccattt aaaacaatac
     5581 agattttcat tattaaattt aaactaattc ttcaagagaa gcattctttc taaataaatt
     5641 aactgtttct tgaatgtcat cacttccaaa tattgcagaa cctgctacta ttacattagc
     5701 tcctgcttgt acaacttcgg ctacgttttt agggcttata ccaccatcaa cttctatatc
     5761 aatgtttaat ccttgactat ctataatagc ttttaactct ttaatcttat ctaaaacaca
     5821 aggaataaat gattgtcctc caaatccagg gttaacagac attaataata ccatatctaa
     5881 atcaggtagt acatgtttaa tcgtatctac tggtgtagct ggattaagta ctacaccagc
     5941 ttttattcca tgtgatttta tattttgtat agttctatgt agatgaacac aagcctcctg
     6001 gtgaacagtt attatatcac atcctgcttt tacaaattct tcaatatact ggtctggatt
     6061 ttctatcata agatgagcat caaataccat atttatattt tctttcttta aacttttgac
     6121 tacaagtggt cctaatgtaa tgtttggtac aaagtgtcca tccattacat cgatatgaag
     6181 atattcacat cctgcacttt ctacttttct tacatcttct aataattttg caaaatctgc
     6241 tgataatatt gatggtgcta atttaatcat ttttctaata cctcctacta ttattttgtc
     6301 ttatttcatg tagcagctgt atataactat catatctttc tttagatatt tctccatttg
     6361 taacagcttc ttttatagca caattaggtt cattttcatg aatacacttt gaaccaaact
     6421 tacaatcatt aaatttatca aattctataa aataatcttt aagttcaact tcctctatat
     6481 cttcaagtgc aagggaacta aaacctggtg tatctgcaac cattccacca aattccaatt
     6541 ttaaaagttc agcgtgacga gtagtatgtt ttcccctttt tattttatca cttaccactc
     6601 ctgtttgaag tttaaaattt tcatctattt catttaataa gcttgatttt ccaactccag
     6661 atggtcctgc aaacactact acattttctt ttaattcttc ttttacttta tctatattta
     6721 acttagtaat attgcttaca ggaatgactt tatagccact aagttcatat atatttttaa
     6781 cagtttctaa tgtatcatta tcatctaaat ctgcttttgt aaggattatt acagtttcta
     6841 aattttcttt ttctgccaaa actataaatc tatctaaaag agataagttt ggtttaggat
     6901 tttttattgc gaatactatt aatgctttat ctacatttgc aataggaggt cttataagtt
     6961 ctgtatctct actgtcgatt tcttctaaga tacccttttt ttcatcttca tcaacaatgc
     7021 ttattttgac tctatcacca accaaaggtg ttattttctg ctttctaaat atccctctag
     7081 ccttacattc ataaatacca ttataagtat ctacataata aaatccacta atccccttta
     7141 taatttttcc ttctagcatc aaatgcctcc ctttataaaa tttatataaa cacatactgt
     7201 ttatgaatag tatatcgtgt tattctttcc tatttttata taataccaca ttttttttaa
     7261 ataacaaatc ctccgtttgg actaattacc tgaccagtga taaaatttga tttgtctgat
     7321 gccaagaaaa tagcacaatt tgctatatct tctggagttc ctagcctcat tagaggggtt
     7381 tcttccacaa gagcgtctat atcttcctca ttatatccag ataacatgtc agtattaatg
     7441 acacctggag ctatgctatt tactctaata tttgatggtc ctacttcttt agctaatgcc
     7501 tttgtcatac ctattatacc tgcttttgtt atagaataat gcacttcaca agacgcacct
     7561 gatatacccc agattgaaga tatattaatt atatttcctt ttttttctga tatcatgtat
     7621 tttaaagcaa cttgcgaaca ataaaagctt ccttttaaat taatattcat catattatcc
     7681 caatcttcat cggttatatc tgtaaacaac ttatcttgac ttacaccagc attgtttaca
     7741 agtacatcca atcctccaaa ttcttttata caataatcta ccatattctc tacatcttct
     7801 ctatttgaaa tatcagcctt aaaaagtttt acagaaaaat ttttttcatt taatatagta
     7861 tacaactctt tagcttcatt ttctgactta ttaaaattta ctaatacatt gtaaccttct
     7921 tttgcaaaag ctttagacat agctttacca atccctctag ctcctcctgt aattaaaacg
     7981 gttttcttct tcataaatac ttttctcctt atattataac caattcagta ttttatacaa
     8041 aacaaactgc cctttaaaga aaatattttc tataaaagga cagctcttat tttattaata
     8101 tttattctgt tttaccaatg tttgcgccag ttcctgatga agttcctcca ttatctcctg
     8161 agtttgagcc tcctgaacta tctccattgt ctcctgagtt tgaacctcct gaacttcctc
     8221 cattatctcc tgagtttgaa cctcctgaac ttcctccatt atctcctgag tttgagcctc
     8281 ctgagcttcc tccactattt cctgagtttg aacctcctga gttacctcca ttattgcctg
     8341 agtttcctcc actatttcct gagtttgaac ttcctgaatt tccaccactg ttccctgagt
     8401 ttgaaccagg ttctgttggt gtattattct ctggtgttgt tggtttttta tctggtactg
     8461 ttggagtctg tgtatttgat ttttctgaac ctttgcttac atataggttt acctcatcac
     8521 cttcctgagc tcttgatgag ccagcacctg gactctgact taaaactgta ccttctttat
     8581 atattgagct atattcatac ttaacagttc ccacttttaa gttattttct tcaataagtt
     8641 gttttgcatt actcaaagta agtcccacca cattaggaac agatgcctga cttgaacctt
     8701 tacttacgac aacatttata gtatcaccct ttttgacatt tgtacttcca ccttcaggtg
     8761 tctgtgatat tatacaattt tcatcaactt cactattata ctcttcactt tttacactca
     8821 ggtataatcc ttctttttca acagcacttt gagcttcttc taatgtcata tttacaagat
     8881 taggaacagt caaagattta ttgccaaacc ctccagcaaa taaaaactta tatgctaaga
     8941 atacttgagc acacaatatc agtatcaaaa cagccgcaat tacttttaat cttcttctag
     9001 attttgggct ttcctgtttt ttcttctttt tcttagttct cttacttgga gtactcttag
     9061 gtctctgatt ctttttagct ctcattatct cttcctcttc ttcttcttct tcttcatctt
     9121 cttcataaaa atcatcatag taatcttcat catcatccaa atcagctact tcaacaggct
     9181 taaccacttt ttcaggagct ggttttgcaa gtgttggatt tacaacttta ttaatttctt
     9241 tttcatcaat tttcttagtt gcaaaatcat catattcttt tataaaatct aaatcaatat
     9301 ttttttctat atactctata tcttctatca attcttcagc agtttgatat ctatctgcgc
     9361 tagatttttc tgtaagcttt tttattgtag ttcttacact ttgaggaatc ctaacttttt
     9421 cttctgatgt aaagtctata tcatcattaa tgtgttgaag tgctatagaa attggactat
     9481 ctcccctaaa tggaactttt cctatcaaca tttcatataa aactatacct agagaataca
     9541 ggtcagcatt attagttaca aatttacctt tagcttgctc tggtgaaaaa taatgcactg
     9601 aacctattat actaccaata ttagtcattg ttgaatttga aacagctttg gctataccaa
     9661 aatcagccac ttttactact cttccttcat ttgatatgag aatattatga ggttttatat
     9721 ctctatgtat aatccccttt ttgtgcgctg cactaagtgc catagctatt tgttttgtta
     9781 tatctaatgc agtatattca tctaatatac cttcatcttg tataatctct tttagattct
     9841 tcccatctac aaactccatt acaatgtagt gaacttttcc atcttcacca acatcataaa
     9901 catttactat atttggatgt gaaagacttg ctactgcttc tgcctctctt ttaaactttg
     9961 ttagaaattc atcatcatct acaaattcag gtctaagtac tttaagtgca acagtcctgt
    10021 ttaataatct gtctttagcc tcatatacaa aggccattcc tccatcacca atcttcctga
    10081 tgatttcata tcgatttcct aaaattgtat ctcccacaat atcaccgtcc taattatact
    10141 tttatcacta taaccgaaac attatcttta ccagaaacat cattggccat acttatcaat
    10201 tcttcactta tattactagt tctctcatat tcataatcta atattaaatc tcttatatct
    10261 tcatcatcaa taaacccagt taatccatct gtcgcaagaa gtattatatc actttttttg
    10321 atttctttct taaaaatatc tacaacaacc atatcatctg ttccaattgc tctagttatt
    10381 ctatttctct gtggatgtct tcttgcctct tcttctgtaa tgacatgggc catcataagt
    10441 tcttcaacca cggaatggtc tatagttatt ttatcaaatt tttcatctgt taacaaataa
    10501 catctgctat ctccaacatt tgccacatat aagacattat tatacactat agccgtaact
    10561 aatgtagttc ccattccttc aaattctata tcttccatag attttttgtg cacaattgag
    10621 tttacattat tataagcctg ttttaatata tcatctatat agtctatttt tacattatca
    10681 tgttgtaata agttttcctt tagaaaatca attatatttt ctactgctaa tttactggcg
    10741 acttcacctt ttttatgtcc acccattcca tcagctatgg caaatattcc tattggacca
    10801 tgttctgtat ctataacttc tccctcacaa tagtcttcat tgttttttct tatttttcct
    10861 atatgggagg cacaactata aaccatacga cttcctcctt acaaattact tgtattttct
    10921 tctcaattga ccacaagctc cactaatatc agaacccata gacatcctaa ctgtggcagg
    10981 tatattgttc ttctccaaac tatccctaaa tttataaata aatgctttat ctggcttttc
    11041 gtattctctt tcttcaactt tatttattgg aattaaattt acatgacata gcataccttt
    11101 taggagctta gctaatgcct ttgcttcttt ttctgagtca tttactcctt ttataagaga
    11161 atattcaaat gtaacccttc tatttgtctt ttttatataa tatctacatg cattcaatat
    11221 ttcttcaata gaataagcat ttgctacagg cataattttt cttcgttctt catcatatgg
    11281 tgaatgaagt gataaagcta aatttatagc tatttctaag tctgccaact catacatttt
    11341 aggtactatt ccacaagttg aaagagttat atgtctatac cctatattaa gaccattttt
    11401 ttcgttaatt atttttaaga attgttttgt attttcaaag ttatcaagtg gctcaccact
    11461 ccccatcaat acaagatttg ataccctctt accagtatct tcttgaattt taataacttg
    11521 gtctaaaatt tcccatggct ctaagtttct aattaatcca tccattgtag atgcacaaaa
    11581 attacatccc atcctacaac ctacttggtt agaaacacag acagtaactc tactatcata
    11641 atccatcatc acagtttcta ttatatttcc atcatctaat aaaaacaaat atttttttgt
    11701 attatctacc ttagattcta actttaattc tatatctata ttccctatac atgaaacctc
    11761 ttctaattta tttctcaaac tttttggtat attgttcata tcatcaaaag tttttgcacc
    11821 cttatatatc caagagaata tttgacttcc tctaaagggc ttttcatcta tagttttcat
    11881 aaattcttta agttcatctt cagtaaaatt ttttaatact atctttttct tttccattct
    11941 ctcactacct tattctcttt aattttgcta tgaaaaaacc atctatacca tgaatattag
    12001 gatatatctt taaatatcct ttatcttgat tgtctaagtc tacatttact gtatcaattg
    12061 gaacaaattc aaagttgtga ttttcttcaa caaaagaagt aactatattt atattctcca
    12121 catcttgaat tgtacatgta ctatatacta aagttccacc aactttgaca tatttagatg
    12181 cattttctaa aattttcttt tgtatagaag tgatgtcttt taactcttct tctttcttat
    12241 acttaatttc tggtttacgt cttattatac caaaaccaga gcaaggaaca tctgctaaaa
    12301 cataatcaaa tttacttata ctattttcat caatctcaga agcatcaaat ctttctacaa
    12361 acacattttt aagccccaat ctatttacag tagagtttat aagctttagt ttatgctcaa
    12421 aaatatctct agctactact tgacccgtat tgttcattaa agtagctaag tgagtagatt
    12481 tccctccagg agcactacat acatccaaaa caaatgaacc ttcttttgga ttaattactt
    12541 tccctactat catagaactt atatcttgaa ttgtaaataa accagcctta aataattcat
    12601 ttttttctat atttttcatt ttttcgactt taatagcttc ttctaccata ggaactttta
    12661 aacatacaat acccatttca cttaattttt caataagttc ttctctactt attttaagtg
    12721 tattcgttct tatatagata ctaggctttt cattatttgc ttccaacaaa tcttcagcaa
    12781 attcttgtcc aaatttatct atccagtttt ttataatcca agagttatag gaatacttta
    12841 tagataagta ttttacacta tcttcctcag ttatgtccat tatagtctct ttagctctta
    12901 tttcatttct aagaatagca tttatgaaac cagaagattt tttatcatat ttcttagata
    12961 attttacagt ttcattaact gctgcataat ctgatatact atctaaaaat aaaatctgat
    13021 atgttcccat tcttaaaaag attttaacat aagttgacat tttttttacc tttatttttg
    13081 aaagtttatt gattatataa tctaaataat atttattttc tattactcca taaattaact
    13141 ctgttgcaag tcctctatcc atatcactta tttctaaatt tttaaaatgc ttatttattg
    13201 caatatttga ataattgtca tttttttcta tatcacataa tactttaaat ccaacttctc
    13261 ttgcatccat ataaatttct cccttttatt aaacataaaa aagggttgtt tctaaacaac
    13321 ccactttact tttagtcatt tcttctttga gctattagta acaatctaag taattgaagt
    13381 attgaaacca atgctgctgc aacatatgtt aaagctgctg ctgaaagtac tcttctactt
    13441 tgtcttgact cactttcatc tataatacct aaattcccaa gttgaacgat tgctctactt
    13501 gaagcattaa attctacagg caatgttatt atttggaata acactgaagc cgaaaacaat
    13561 agtataccaa tttttaaaaa tggaccagcc ataataaatc ctaaaaatat taaaaaccaa
    13621 gatatacttg aagcaaaatt cactactggt actaaactac ttcttatttg aagaggcgca
    13681 taacctttag catgttgtat ggcatgacca cactcatgtg cagctactgc aacagaagtt
    13741 atagatgttc cataatatac atcttctgac aaccttacag cctttcttct tgggtcataa
    13801 tggtcactta gatgccctct aaccatttct atctttacat catacaaacc atttgaatca
    13861 agtactcttc ttgctgtttg ctctccagta tatcctcgac gtgtatttac tcttaaatac
    13921 ttatttgttg ttgaactcac tttaaattgt gcatatatag taagtaatat agctggaatt
    13981 aatacaacca ttgttgggtc aaatccccaa aaaccgccat atacagggta cataaataac
    14041 actctcctaa aatatatttt tattatacat cctcttaata ttcacttaac ttaaaaacag
    14101 tttaattaca aattaatata ttttttcctg ttaaaaagtc tatagctttt tcaatatcca
    14161 tcatgtctac attagtattt acacaaggtc cattaggtct tttgttaaat acacctaaaa
    14221 caggaatttt cttaatatct tgtattccag atgtcaaatc tctttcacat gcaacagcta
    14281 caacagcttt aggtttatta tccattataa tttttctagc tagggttcct cctgtagcta
    14341 caaatatttt tacattagtt ttttccttta gcttaattaa ttctcctacg ttgcatcttc
    14401 cacattcttt acaattatct atatcattag taacttttaa tttacaacta ttctcttgaa
    14461 tacaatgagg tattaaaatt attatatcct ctggattaaa attatattta ttactgtata
    14521 tatattcatt atttaacttt atatatattc ttctaatatc actttttgac ataccaaatg
    14581 atgatgcaat gaaagatata ataggaaata atccactgac aatattaaaa tttattttca
    14641 ttatatttga gttaacatac tttcctttaa ttactctata agtaactgct gttgaacata
    14701 gtattataag tattagtgat attattataa tatttatcat caaggagaat atttgagtta
    14761 aacttattat aaaaaaatta acaactccta tcaaaattcc taatattaaa atcaaaatcc
    14821 caacacttgt taaatacttc tttatatctg tcatactttt cacctaaccc aatactaaac
    14881 cagtttctat agtattccct tttatatatt caccaaccaa cattctcttt ttattcggca
    14941 tctgaatttc ttcaattaaa agtacattat ctatagtact tacttttatc ccatctttgc
    15001 ttacatcaat tattgtacct acatcttttg tgcttttttc atctaacact ttagttttcc
    15061 aaactttcat tatgacatca ttatagatag tataagcact tggccatgga tttactcctc
    15121 taactaaatt gtgtatttct ctggcacttt ttgaaaaatc tatgttacct aatgatttat
    15181 tcattattgg cgcataggaa aattcttcat gattttgtac ttctcttgga gcatttcctt
    15241 cttcaattaa tcttaatgtt tcttttaaag tttctgctcc tatattcatc attttatcat
    15301 gaagttctcc tgctgttata ttttcatcaa gatttacttc tgtctttagt atcatatctc
    15361 cagtatctaa cccttcatcc atatacatag ttgtaacacc agtcttttct tcaccattta
    15421 ttattaccca atttataggt gccgcacctc tatattttgg aagtaaagaa acatgaacat
    15481 ttatacatcc aagtttagga atctctaata tttcttttgg aagtatctgt ccaaaagcta
    15541 caactactat tacatctgga tttaaagatt taattttatc tataaattct ttatctctag
    15601 cctttactgg ttgatataca ggtatattat tttcaattgc cagttctttt actggactca
    15661 tacctagttt tttacctctt ccttttggtt tatctggttg agttacaaca cccaatattt
    15721 catacttctc atctattatt ttttgtaaac atggaactgc tatatcagga gttcccataa
    15781 atactatttt catcaaatca actcctacta cttttctatt ttgtcaacaa ataaaattcc
    15841 atctaagtgg tctatctcat gacaaaatgc tctagctaaa agttcttctc cctctaactc
    15901 tattgtttcg ccatttctat ttaaagctct gacctttaca taatttggtc ttctaacatt
    15961 tccagcttct ccaacaacac ttaaacatcc ttcctcatct atttgctccc cagatgtttc
    16021 tattatttca ggatttataa gttctataag ctcttctcca atatctatta ctactactct
    16081 ttttaatatt ccaacttgag gtgcagccag tccaacacca tctgcatcat acatagtttc
    16141 tgccatatca tctaataatt gtattatctt ttcatcaatt ttttcaacct tttttgattt
    16201 ttttcttaat actggttctc ctatttgaac tatttgtctt aaagccatta taattcctcc
    16261 taaatttata atacactgtt tggattaata tctatagata cattgatatt cttatcaaat
    16321 atcaaatctc gttttgttat acatatatat tttattatac cctttaagag attaatttca
    16381 atattttcat ctttaaagag tatttgccat ctataatttt gatttatttt tgatattgaa
    16441 catggatttg gacctaatat aaatcctaaa tcatttatac cccttttttc taataaataa
    16501 atcaatgatg catgcatatt ttttatattt tttatcacta atctttcatc ttcaccacta
    16561 acaactacac ttaacatatt attaaaagga gaatatccaa atgcttttct tatttttatc
    16621 tcatcttcat aaaaaccctc ataatcatat tcaatagcat gcttaatagc atagtgttca
    16681 gtgtcataag tctgaagtac tacttttccc tctttatcag ctctccctgc tcttccagag
    16741 acttgagtta tcaattgaaa tgtagtttca aaacttttaa agtctggaaa atttaatatc
    16801 atgtctgcag acaaaatacc tactaaagtt acattctcaa aatccagccc tttacttaac
    16861 atctgtgttc caataagtac atctgcttct ttatctttaa atttatttag tatctcatct
    16921 aaagcacctt tttttgaagt tgtatcttta tccattctca aagtttttat atctggaaaa
    16981 atatatttta attcctcttc aattttttga gttccaactc caaatggctt aacataagta
    17041 cttttacatt caggacactc ttttggaatt tctttctcat atccacaata gtggcatctt
    17101 ccagtattgc ttttcttatg ataagttaaa gatatatcac aattttcaca ttgaaacaca
    17161 tatccacact ttctacaaga tacaaaattt gcataacctc ttctgtttaa aaatagtatc
    17221 acttgattat tttcttcaat cgcataacgt atttcttttt gtaatttcaa actaaatata
    17281 cttctatttc ctttatctaa ttcatctttc atgtctacca cttcaatgtt tggaagaggc
    17341 ttatcatttg ctctactttt tatatttata agtttgtatt ctccagattt tgccctataa
    17401 tactcttcaa ttgagggcgt tgctgaacct aaaataagag ttatattatt ttttaatgcc
    17461 ataaatctag caacttctat agcattaaat tttggattct tctcagactt atatgatgac
    17521 tcatgacatt catcaattat tattaagcct aaggaattaa aaggtgcaaa taaagctgac
    17581 ctagctccaa ttaacactct tactttacct gctttgacag ctttgtaaac atcatgtttt
    17641 tctccttcag atagtttact gtggaacact ccaactaaat ctccaaacct actctttagc
    17701 ctatctattg tttgaggagt taatgctatc tcaggtacta agaatatact gtctaagcct
    17761 tgatttagag caaattctat tatttccatg taaacttcag tttttccact tccagttact
    17821 ccatgaatta agtatggttt tttatcatct acaaacatat tcgactttat ctcatcaact
    17881 gcttcttgtt gttcattgtt taattttata ctttttagta cggatttgta aatacttttt
    17941 ggctctctat aataatcctt aaattctagg gttattagct ttttcttact tagagaattt
    18001 atactttgtt ttgaagcatt taataaatcc aataaatcat ttatctctac attttcatta
    18061 tttttaagaa aacttaatat ctctttttgt ttacttccca catttatttt attttgctct
    18121 atataatcgt caatcttatc actttctaaa ctcaaagata tataacatac tttcttttca
    18181 tttttgtgat ttttatattc ccaacataat tttataattc catttttatt cattgtgtac
    18241 aaagaactat ttaagtttgg tatatatttt aacttatcaa ccttaatttt acctttacta
    18301 tcaatcactt tctctactat ttctctattt ttatcactta aagttgatat tttttccttc
    18361 aattcaatct catttaatcc acttaaactt tcacctaata ggactacttt ataattattt
    18421 aatttgtatc cctttggata tatcaaattt atacattcta tataagtaca caaatatcta
    18481 tttttcatcc aataaaccaa ctctaaatct tcttttctaa atattggatt ttcatctaat
    18541 atatcaataa tttcttttat ttttattttt tcatctaagc tatctgtaaa ttgaaataca
    18601 aaggcttctg ttggtttatt accttttcca aatggtacta atattctgtg cccgattttt
    18661 attacatcag ataaaaatac aggaatttga tatgtaaata aattatctgt atatatagta
    18721 ttacttctaa ctattacctt tgcatatttt ttcattataa aacctctact taaatttaat
    18781 atattgttca tattttaata tactttacaa gatattaaaa tctcagaatt tataagcaat
    18841 ttcaagttat taatattaac tttattttaa taattaacta ttaattatat tttatacttt
    18901 atgacaaaat aaatgctgaa aactacaatt taactgacat ttgcgagttt ttactcgcta
    18961 aaattaaatt gcagctttca gccactttag ctgtgataaa atacacaaat atcatattta
    19021 aaaaaggtct tatttctcaa gcaacgcttt aactttatct aaaataacat tggctacttc
    19081 ttcttttttc atcttaggat actcagtaat atttccctct acatctatta tttttacaat
    19141 attagtatca gctctaaaac cagcaccttc tttagttaaa tcatttgcca caataaaatc
    19201 taaatttttc ttttttattt ttcctttggc atgttctata aggtcattag tttcagctgc
    19261 aaatccaatt agtatcttat cttcttttat ttttcctatt tcataagcaa tatcataatt
    19321 tctacttagt tctattgata aatcatcatt cttcttttta atttttttgt cagaataagt
    19381 ttttggttta taatctgcaa ctgctgcact ttgaattact attttatttt cacttaaatt
    19441 tgatactact gcttcataca aatcttgtgc tgactctatt tgaacaaatt ttttaagatt
    19501 ttgtggaata gaaagatttg taggacctga tattaatgtt acatcagcac ctctagctat
    19561 agcttctctt gctatagaat atcccatttt accagaagac ctattagtta aatatcttac
    19621 tgggtctata ctctctacag ttggaccagc tgtaactatc atactttgac cttttaaatc
    19681 ttgctcttta gtaagattag tcaaaacttc ctctactatt atgtctggat tagctaattt
    19741 gcctgttcca atatctccac atgcaagcct cccactttct ggttcaataa atttatatcc
    19801 aaagctcttt aaagtttgta tatttttttg aacaattgga ttttcataca tattagtatt
    19861 catagctggt gctataagaa cctgtgcttt ggttgccata acagtagttg tcaacatatc
    19921 gtcagctata ccatttgcaa tcttgcctat aacatttgca cttgctggaa ctattaagaa
    19981 taaatctgct ttttttgcta atgatatatg ttcaacttcc caagtttttg gttcttcaaa
    20041 catatcttct actacataat tttgactcag cgattgaaat gcaagtggtt taacaaattc
    20101 tgttgctgat tttgtcatta taacatggat atttgcattt agttttttta acttacttac
    20161 aacatcacaa gccttatatg cagctatacc gccacttatg ccaattacaa cagttttatc
    20221 ttttagcatg accttactcc tcttctgaaa tttgttggtg ttgctcagca tgatgtctag
    20281 cttcttctat ctctatttcc tcttctgtca ataatctata tgttattttt ccacttgcaa
    20341 cttctttagt tgctacacat acaggctttt ccttagtttt atttgaaaca tatggttctt
    20401 ctccatcaat taattttctt gctctctttg aaacagtccc tactaaatag tatctgttgt
    20461 ctattttttc tagtacctca ttgatagatg gttttaacat aattataatt cctccttaaa
    20521 tttctctatt ataatatttt tatatctgct aactttattc ttttcagatg atattatacc
    20581 ttctatttct ttagcagatg tttttacatc ttcattaaat ataaaataat cataatcctt
    20641 aatcatttta atttcttcat atgcacagct aaatctcttc tctatttcag cttctgtttc
    20701 tgtacctctt ccaacaattc tattttttaa ttcttcaagt gatggtggta gtacaaatat
    20761 aaatactccc tctgggcaaa cttcttttat ttgttttgct ccttgcattt caatctccag
    20821 taatacatct tgtccttttt ctaaacattc cattattgcc gcttttggag ttccataaaa
    20881 attatcataa atttgagcat actccaaaaa ctcacctttt tctatcatct cttcaaactt
    20941 ttccttactt atgaagaaat aatttactcc atcaacttct ccagttctgg gttttcttgt
    21001 agtagctgat acagaaagtt ttattgtatc attttcttta agtagttctt tgcaaatagt
    21061 accttttcct gcacctgatg gtcctgatac aactagcaat aatccttttt tcacaaacat
    21121 ttaagcattc cccttctaat attgtattct tactctatat tttgtatttg ttctcttatt
    21181 ttttccaatt cgctttttac ttctactact aaattagtta tatttatatc agatgacttc
    21241 gaacctatag tgttagtttc tctattcatc tcttgtatta agaaatctat ttttctacct
    21301 atagagtcgt ctttaaatat agtattttta agttgcccta tatgactttt aaatctaact
    21361 atttcttctg ttatactgct tttatctgca tatattgcaa cttcttgtgc taatctattc
    21421 tcatctatta tacttgggtc atccagtaat tcacttattc tcaaattcaa tttttctcta
    21481 taatcttcta caacactact tgaatatttt tcaatctctt ctatatgatt ttttaataaa
    21541 tcacatctca tggcaatatc ttcagctaat tttttacctt cttcacttct catttctctt
    21601 aatttgatta atgcatcttc aactgcttga ttcaacattg accacaataa atcttcatct
    21661 tcttcctttt cttctatttt cacaatatca ggaaactttg caacattcat aacgctaata
    21721 tcatctataa tatcaaattt attttttatt tcttttagta tgtctatata ctgagatgct
    21781 aatccctcat caaaatttaa gtttacatct tctttgccta ataagtcaaa ctttatgtat
    21841 aaatctactc ttcctctttt tacataattt tttactaaat ttctaacttt atcctctaaa
    21901 aatgaaattt ttcttggaag acgtatgttt atatctgaat acttatgatt tatcgtttta
    21961 cactctacta aaaagtaata attatcatcc ttgtattctc ctcttccaaa accagtcata
    22021 cttatagcca ttaattaaac ctccaaaaca ccttcacata tttttactgc tggacctgtc
    22081 atataagaga aatctcctac atctatttta actgttccac cttgagacgt tacatttaca
    22141 ctactttcta ccatattaag taaattgcac actattgctg atgcagtcat accagtacca
    22201 caacctaaag tatatccaca acctctttcc caagtataaa catgtatatt atacttatct
    22261 tctaccttta caaaatttac attcgttttt tcaggaaata ttttattatt ttcaatctct
    22321 tttccatatt tacaaacatc atctacatct aattcatcta ctataactac tgtatgagga
    22381 acccccatca acatagaagt aactttaagc tccctatcta aaacctttat actttcttgc
    22441 acaaaacgtt ctttatttgt aagtacagga acatccttag ctataaaact tccttttccc
    22501 atattgactc ttatagagtt tatttctcta ttggtaagat ttatttttat atcttttata
    22561 ccatctaatg tatctactgt aaaactttct tttttaacta tacaattatc ataaacaaat
    22621 ttacaaaaac atctaaggcc atttccacac atagctgctc ttgaaccatc agaattataa
    22681 tataccattt ctacatctgc aacttttgaa tctttcacaa ctaacaatcc atccgctcct
    22741 acagaaaatc ttctatggca tacttttttt gctaaagcag tataatcaga tggatttatc
    22801 tggtcgaatc ttccatctat tgctataaaa tcatttccta ttccatgtag tttccaaaat
    22861 ttcataagta tcgctccttc gtaaattaac gcttaaacta ttataacata aaaaaattcc
    22921 cataaactta taatttatgg gaaaagtttt gtaatgacaa ctataatttg aggatgtgag
    22981 ggagagttgt cattacaatt agggtaatta tattatgttt ttaagctagt tctttctatg
    23041 tttatatatt aatatttcta tatgaatttt atgtatgttt tatttaaact gtctatgaat
    23101 tttttaatta aacatctggt aaccagctat aatcctttta gtagatatct attaacatac
    23161 acctattaat tctttaagtg tttttaaaac atagtctaaa tcttccttag taatatctac
    23221 atgagttaca aatctatatt caccattttc cattttatta atttttatat ttctttcaaa
    23281 aaattctttt attaaaacat cctcttttat aatatcttca ctcatcttaa aaaataccat
    23341 acttatatct ggattaacat tgtttacttc aatgccatca attttattta attcacaagc
    23401 taaatatttt gcattttcat ggtcttcatt caatcttaaa gtcatttttt caatggcaat
    23461 tataccagct gctgcaacta tacctacttg tctcattccc ccacccataa ctttttgctt
    23521 tcttctagct tttgctataa attctttact tccagcaagt atagaaccca ttggtgcagc
    23581 taaacctttt gacacacaga acataacact atcacaacac tttgctattt ctttagcttc
    23641 tacacccaaa gttaatgctg cgttaaataa tcttgctcca tctaaatgta ctggtaagtt
    23701 gtgtttctca gctatttttt taatttctct taaattatca agactcacaa cagttccacc
    23761 tgaatatgca ttttctacac atataagccc tgtctgtggt tcatgtatgt catctcttct
    23821 aattgccttt tcaacaagtt caggatttag agctccactt tcgtcttcat ctatagttct
    23881 aagctgaact ccagctatta cagcaggtgc cccaacctca tgagttacaa tgtgataacc
    23941 tttaccaata attatttctt gccctcttag gcaatgagta aatagagcta attgattccc
    24001 aaaagttcca cttggaagga aaatagcatc ttcttttcca actttagctg ctgctaattc
    24061 ttcaagttta tttacagtag ggtcatctct aaagattgaa tctccaacag gagcctttgc
    24121 catagcttct ctcatttcgt cagtaggcat agtaacagta tcacttctta aatcgataaa
    24181 tctcattttt tcaaccttct ttcatattta tatactatag aaccttaaaa ttgatttttt
    24241 ctgtaaagtt catatacttt catagcttat actctacaca ataattatac tatatttact
    24301 ttatatttaa tctactttaa attattaatt ttaaaaataa agtttcctct ctaataagta
    24361 taaataatta tacaatttaa atattaatct ctatcgttta aattccccct agtcacaaag
    24421 cgtatattat gtgtataatt aatttactga ctcattttgg tcaaagaaag gggtgctata
    24481 aatggcatta gatggattag ttatacattc aatagtcgat gaattatcat caaaacttac
    24541 aggtggaaaa atagataaga tacatcaacc tgaagatgat gaggtaattt ttaatataag
    24601 aaataataaa gaaaatttta gattggtttt aagtgcaagt gcctcaaatc ctagagttta
    24661 tcttacaagt aattatcaaa aagaaaaccc tctaaaagcg cctatgtttt gtatgttact
    24721 tagaaaatac atacaaggtg gaaatatagt agaaatttct caaataggtt ttgaaagaat
    24781 aataaaaata agtgttgaat ctttagatga attaaaagaa aagacagtta aaaatattat
    24841 gattgaaatt atgggaagac atagcaacat aataataact catggtgaag aaaataaaat
    24901 tatagattct ataaaaagag tcccttttag cataagtaga gttcgtcaag ttttacctgg
    24961 tcatgattat tcattaccac cagaacaaaa caaactcaac cctcttgatg atatatctaa
    25021 ggatttgttt attaaaaacc tagaagaatt ggaaggacct atctttaagt ctatatattc
    25081 taaatttcta ggtataagcc ctattatagc taaagaaatt tgctacagag caggtgtaaa
    25141 tcaaaatgct atcataaagg atatatctga tgaacaattt gattctttac acaaagtatt
    25201 ttgtaattta ttcaatgata taaattctaa caaatactcc ccttgcatta ttatagataa
    25261 aaaggtggat aaggtagttg atttcagttg tataaacctt acattattta gcgatttatc
    25321 atatataaat aaggatagta tgtctcgtat tctagaagat ttctatagaa ctaaagatat
    25381 taaagacaga attaatcaac gttcatctga tttaaagaag agtatatctg ttaagcttga
    25441 tagactttat aataaactta aaaaacaaga agaagaatta tcagaatccg aaaatgcaga
    25501 catttataag ataaaaggag aattgattac ttcatatatt tatatggtag aaaaaggtat
    25561 ggaaagtatt gaaattgcta acttctatga tgaaaattgt aatgatgtaa caatagaatt
    25621 aaataaaaac ttgacaccat ctgaaaatgc tcaaaaatac tttaaaaagt ataacaaaat
    25681 gaaacatgct aaagtcgaga tatctcatca aatttcctta aataaggaag aaatagatta
    25741 cttagaaaac atagttttaa gtattgaaaa ctgcgaaaat ttagctgaat tacaagatat
    25801 aaaagaagaa cttgctaagg ttggatatat caagactcaa aagaaaaaca gtaaaaagga
    25861 tactatccca tctacaaaac ctcatgaatt tgtgtcttct gatggattta aaatattggt
    25921 tggtaaaaac aataagcaaa atgactatct tacactaaga cttgctgata atgatgactt
    25981 atggatgcat acaaagaata ttcctggttc tcatgtaata ataaagtgtg ctggtaagga
    26041 agtccctgat aatactgtat ttgaaggagc tatgctagca gcattcttta gtaagtcaaa
    26101 actttcttct caggtgcctg tagactatac taagagaaaa aatgtgaaaa aacctagtgg
    26161 ttctaaacca ggtatggtta tttatgaaac taacagtact atttatgtta ctcctgaaga
    26221 agaaacagta gccaagctta aggttaaatc tgaataaatt ctgcactaaa tacaaaataa
    26281 atgttagaca agcacacatt atcattctaa ataaaaatag tggccttctt ataaagtaca
    26341 ataataaggc cactttctaa ttagcttact tttaattttc taacaaaata cttttctgtt
    26401 ttaataacta tacttatatt cccaacaaat attttcagtc ttcttatata gatgtaatta
    26461 aaaagttaac actaaaagct attttttaat ttagtgattt gttttagtaa ttcattatat
    26521 tctgattcat acaattcttt ttttagatta tctttttcta tagataacat agatataaca
    26581 gttgtaagtc tattttctaa tactagaaga ttctcttttt tgatttgctt gtctctacta
    26641 ggagtttttt tatttctgct aattatatat tcgtcataat tacaatcaaa ctcccttatt
    26701 ttagcatctt ttatctctat aatatagtta caaatatgtg atacaaatac tctatcatga
    26761 gacactataa gcatagtttt atcagtattt atcagtgcct tttccagtgc ttccatagat
    26821 tttatatcta agtaatttgt tggttcatca aatactaaaa aattattatc ttccaatatt
    26881 attttacata gtgctatttt taccttttct ccaccactaa gtacttttac ttttttgtag
    26941 acatcatcac ctttaaaccc aaataggctt aagtttatcc ttataaaaga ttcatcaaat
    27001 gaactattaa ctttagtatt gtatagaaca ctcttttcct catctaataa actttgattt
    27061 tggtcaaaac aacctacttt taccttgtta tttattttta tattgtcatt tttatcagcc
    27121 aatatttctt ttatcagagt actttttcca cacccattat cacctaataa tgctatcttt
    27181 ttacctcttt ttatcttaaa ggaaacatta tctaacaaga gcttattttc taactttaga
    27241 gtcatatctt ttacctctaa tagatttttg ctaattatct ccataccatc ttgaatctta
    27301 atgttcattt ctttagaaac tttaggttta ttctttacct caagtttatc aattctgctt
    27361 tttatagcct tcacatttga gtccaacttt ttcttattag tctgaccacc cattttgtgc
    27421 aacctagctt ctgaattacc cattcttttt ggtgtctttc ttatgccatc acttaaagct
    27481 tttttatata aaagagcttt ttcaagcctt tttttctctt taacatattc ttgatactca
    27541 aaatcagctc tttttacttc ttcatctttt tgcattaaat aatcagtgta attgcctttg
    27601 taagctttta gttttccatc ttctagttct aatatagttg tacataactc atccagaaaa
    27661 cgtctatcat gagatattaa taataatgct ccctcatgcc ttttaaacat atcttccaaa
    27721 agcccaatgc tttttttatc taagtttgaa gttggctcat cagctattat gatatttttc
    27781 ttattactca gcgcatctgc tatttttaac ttaacctttt cccctcctga taagtattct
    27841 tcaaatttat ctggagcatt taaaagactc ttctccttac tatgactaca tgtttcaata
    27901 ttattttcac tctgacttat ataagaatag ctttctgtaa ggtaagtata tccttcatca
    27961 atttctattt cacccaatat tgcttttaat aatgttgttt ttccttgacc atttgaacca
    28021 actagaccta ttttgtcatt ttctaatatt tcaagtttat ctatatccaa aattaattta
    28081 tctgcataat atttctttaa attctctact tttactaaca acataaaaaa tccctcctta
    28141 acacacttaa atataaggag agattctata aataggcata acaaataaac gtatagacta
    28201 taatctactc tccttaattt aagcattgca aataaagcca tcaaaatagg cttttcaaaa
    28261 attacaatac tttaaattat aaaaaataga ttatattctc attgtcctag ttaccttgcc
    28321 ctttctttca tttattttta atcattatat caacattaat tacaaatgtc tatagaaaat
    28381 taactaattt tagttattca atatacaatt tattctttaa atttattctt cttcaacttt
    28441 aaaatcctga ttatttaaaa ttctgtttaa tacaattcca acaattgcag ctaaactaag
    28501 accagttatc tttactgtac ttgttatagg gattccaata gaaatgcctt tattagataa
    28561 atatgttgtt ccaagaccta ttaataatat aactgctatt attaatatat ttatcttact
    28621 ttctacacaa gaactgtccc ttatagtttt taatcctaca taagttatca ttgaaaatag
    28681 cattatacta attccaccca taactgaacc tggtatactt tgaagaaatc caccaaactt
    28741 tcctacacat gcaagcaata tagcaaatat tgctgtacgt ctaagtattg aagggtcata
    28801 gtttttagtt atagcaagta ctgctgtatt ttctccataa gttgtattgg ctggtcctcc
    28861 taaacaccct gcaactatag tcgcaaatcc atccccaagt aatgttctat ttaatcctgg
    28921 attttctata aagtttttac ctactacagc accattagta gtcatatctc ctatatgttc
    28981 cataaataca gctaatacaa ctggagatat aatcacaatt gctcctaaac tgaacttagg
    29041 aactgtaaag ttcggtattg caaataaact tgcttctgta atagtagtta tatctactaa
    29101 ccttaacagt aaacagatta tatacccaga aaaaacggct attaatattc ctaattgttt
    29161 tataaatcct ctaccaaact tattggttaa aattgctatt gctaaagtta taaatgctat
    29221 tacaaaatta gctgaagcca tactaaaagc tgttggcaat aaatttaatc ctataactac
    29281 tatcatagct cctgtaactt gtgcaggaaa atataattta attttattta caccaactat
    29341 ttttactata atcgacatta taatataaat taatcctgct actattatac caccttgagc
    29401 atatgctaaa tctccaccat acgcctcttt agctgctagt ataactggta taaacgcaaa
    29461 acttgaacct aaaaaggcag gtacttttcc ttccgtacaa aaatgaaata tcaatgtacc
    29521 aacaccagca cagaaaattg caactgacgg attaaaacct gtcaaaatgg gaactaatac
    29581 tgtcgctcca aacatggcta atagatgttg taaagacatt attgtttttt tcataaaaac
    29641 ctccaaaatt attatatata aataaatcct ctgtttaatt aatatctttt aattaattat
    29701 cattccctag attctatttt ttaacctcta tatacttatt atataattta tcattatttt
    29761 acattttacg acatcaaaaa ctcaaataaa ggagaataaa aactacatta agactcacat
    29821 ccataaaatt ataatattta ttttgtgcaa tctatgtaag ctttatcctc ctccctgact
    29881 attcaatgct aagcttttat ataatactta aaactaatta ttctttgatt gtaactgaat
    29941 cttctccatc aatttctaat aaactgacag atataatttc atgtctagaa gtaggtacat
    30001 tttttcctac ataatctgct cttataggca actctctatg ccctctgtcc actagaactg
    30061 ctaattgtat tgatttaggt cttcctatat ctataatcgc atctagtgaa gctctcacag
    30121 ttcttccagt gtataaaaca tcatcaacca aaactactgt cttatcattt acattaaaat
    30181 ctaaataagt tcctttaact acaggttcaa catgaatttt ttccaaatca tctctatata
    30241 aagttatatc aatatctcct gtatctactt ttgttccttc tatttgttct attttcttag
    30301 aaattctatt tgcaattggc acacctctag tttttattcc aactaaaact agattttcta
    30361 cacctttatt tctttcaatt atttcatgac ttattctagt aattgctctt gctattgcct
    30421 tttcatccat taattgagct ttttctacca tatcataatt attgtcaaag atataatata
    30481 tctatttgac aaatctactc ctttctttta attttttata aatttatttt tatttaaaat
    30541 ttcaatttaa acaacaaaaa acttctcatc acaagacaag aagctcttat ataaaaaata
    30601 tacagtgtaa tttagcaatt gtcttacgat atctctgtat cgtttaaaga taatttttta
    30661 atctatcaac tcaaattata tatttatatt tcaaattttt caagcttttt taaacaaaat
    30721 actttaatat aagatatttt atttaaattt cactaaattt atttacttat ataaaaacaa
    30781 aaaatgattt ataagtatta tacaaatggt tattttatct tcttaatagc ttccttaaaa
    30841 tactcaggta attctgaatt aaattcgata tattccttag tggtaggatg tataaatccc
    30901 aacttcttag cgtgcaaagt ttgtcctttt aatttaaatt tagtttcttt tggaccatat
    30961 acaaaatcac ctaaaagtgg gtgatttata gacaaagcat gaactcttat ttgatgagtt
    31021 ctaccagttt caagcctagc tctcatatgt gtaaacttat caaatctttc tacaacttca
    31081 aaatgagtaa ttgcattttt tccatccttt actacagcca tttttaatct atccttagga
    31141 tttctcccta taggtttatc aacagttatt ttatcttctt taaccacacc atagcatata
    31201 aattcatact ctctagttat agtatgttcc tttaactgtt ctgctagaaa attatgtgca
    31261 taattgttct tagctatcat aagaagacca gaagtatctt tgtctattct atgaactata
    31321 ccaggtctaa taactccatt tattgacgac aacttatctt tacaatgata taaaatccca
    31381 tttactagtg tattttcata gtttccagga gcgggatgaa caaccatatc ctggggttta
    31441 tttattatta agacatcatc atcttcatat acaatatcta ttggaatatc ttgaggtaaa
    31501 acctctaata ctttaggttc tggtatctct ataacaatat tatcttcctc tttaactaga
    31561 tatttagctt tttctacttt attattgacc tttacttttc catcttttat aattttttgt
    31621 atataacttc ttgacatatc acctaattgt tctgctagat aaatatcaag tctaacttct
    31681 tcttcctctt caagtactaa aaactctttt acttcatcca ttatatcacc tacttttact
    31741 ttcaaaaaat aaaacatata tacataaaaa cacagttcct acaactacaa atacatcagc
    31801 tacattgaat acatattccc atataactct aaagtcgaag taatctacca caaaacctaa
    31861 tcgtactcta tcaattagat ttcctaatgc accagatata attaatataa ttcccaacct
    31921 tccaaatata tgtactttcc ttgtattaag atagtataat ccaaatactg ttgcaagtaa
    31981 tgcaacaatt ataaatatcc actgattatt ttgtaataat ccaaatgctg cacctctatt
    32041 ttcgacataa gttaaatgaa atacattatt tattattggt attgttgaaa catctaccaa
    32101 attattcaat acccatattt tagacagttg gtctaagcct atgagtagaa ttattattaa
    32161 tatatatagc aatgattttt ccccctataa attatttaaa aaccaatttt atttgaaaca
    32221 cactattatt attatacaaa aaaaatgctc taaagagcaa ctttattaaa attgtatttt
    32281 cttaataaag ctagagcaaa atttaaaata gtaccatctt ttttatattg cagattgcta
    32341 catatttaaa atcatattat tatgtataaa aaatttaaaa ataacatact aattaatact
    32401 ttttgtgtgt tttatagatt aaagattaat gatgtctgta ctttaaactt acaagcacca
    32461 ctaatcttca aacaaatatt tttacttttg cacttgctta tttttatcta aatgttgtat
    32521 aagttcagat actgttacga atttatatcc tctctctttt aatttaggaa ttactacttc
    32581 taaagcttgt atagtttgtg atttatcatt tcttttagtg ttataatcat gtaaaagtat
    32641 tatattacca ttttggacct ttgtaagaat agtatttata attgtatcta caccagggtt
    32701 agaccaatct ctagggtcta agtttgacca aagaattata ttcatgcctt tagaaactat
    32761 aatatcacag acacttttac tcatggctct atatggaggt ctaaatatct ttggttctac
    32821 tcctattaca ctttttataa tattttgtgt atctgttatt tctttttcaa cactaccata
    32881 actatttttt gcaacattta tatgtgtaaa tgtatgattc cctatttcat gcccctcatc
    32941 atattgcctt tttaataatt ctttgttata ttctactttt tctccaacta caaaaaaagt
    33001 tgcttttaca ttatttttct ttaatatatc aagtatttgt ggtgtaaaat cctcatctgg
    33061 accatcatca aaagtcaatg ctagaatctt ttcatcctca tttccttttt ttattattac
    33121 atcttcataa tcgtctttta tattgtcatt taaagtatag gtttcttttg aactattttg
    33181 attatgtgta ttatggttat atgtatatac acctactatg gataataaac atatacatat
    33241 accccatatt gctttcttca tgtcattacc tcttttctaa ttcattctaa caatatatga
    33301 tgtcaggctt ctaatctctc aatgttccat ttttatttaa atcagttaaa tttgttaggt
    33361 cttctgaata aaagttagaa tcttctacat caggattttg cattgcatca cctaataaag
    33421 cattttcttt ttttgcacaa ttgttacata aatttgtatc aggtaatata tctaatcttt
    33481 ctaaatctat ttgattgtga cataaatcac aaaacccata tgttccattt tctattttag
    33541 ataatgctga atctatttga tacagtcttc cttcttcatg atttattaaa ctattttgca
    33601 tattttgcat atataaatcc gttcccatat ctccaatatg attgtcataa ctagataatt
    33661 ctcctgatga atatttttca ctagtatgtt tagtagtgtc tccaaacaag gtattatctt
    33721 tcatatctac gactaaatta gttaactttt ctctttcttt taataattta tctttgtatt
    33781 ttttagtatc cattttcaac acctctaatt ttaatattta tatcataaaa atagtatgtg
    33841 gaaaactaaa ttaaaaatac aataaaaaac aagatttgat tttttcaaaa aaaattgtag
    33901 gtaaacttaa aattcaccta caaattcttt acatatattt ttaatataat gtttttagtt
    33961 tttgataata attacattaa cttgctaatt tatgagcatt ttgctctttt atatccttag
    34021 cttttttaaa aaataaaagt ccaaggatta ttgcaactac aatacctatt ccattagata
    34081 tattaactgg aagtctaaat ccttcttttg cctgcattat ataactaaat gttactacag
    34141 tcataaaaag tgctggcaat gtagttatgt aatgattttt atttgctttg actaaatatg
    34201 cagaaccagt ccacaacatt atcatagcaa gtgtttgatt tgcccaagag aaatatctcc
    34261 aaattatatt aaaatctata aatgtaaggg caacccctac agcaaataat ggcaaagcta
    34321 ttaaaaatct atttttaact gcatcttgat tataattcat tgcatcagct attgttaatc
    34381 tagcacttct aaatgctgta tcccctgaag ttataggaca tgctacaaca ccaagtatgg
    34441 ccaaagcgcc tccaataggt cccattaatc caacagatac tgtatttaca acagttgctg
    34501 ctccaccatc attaaatatt acattaagct gaggtatacc atggaaatat gacatagctg
    34561 ctgctgccca aactaaagct attatacctt ctgcaaccat tgacccataa aatactggtc
    34621 ttgcctcact ctctttctca acacatctcg ccataagtgg tgattgagtt gcatggaaac
    34681 ctgatattgc tccacatgct attgttataa ataagtatgg gaataaaggt gttccatttg
    34741 gattcatatt ttgtaaattt atatttggta tgttataccc ctttattatt aatcctccag
    34801 ctacacctac tgccataatt aataatgctg ctccaaataa aggatatatt ttaccaatca
    34861 atttatctat tggtaaaaca gtagccataa tataataaag tattatcact ccaacccata
    34921 tttgtgcgtt tacacctgtc aatgtcttta ataatcctgc tggacctgtt acaaatacaa
    34981 ctccaaccaa tactaaaaga ataacactaa atattctcat tgctattttt gcaccatttc
    35041 ctagataaag acctaccaac tctgatgcag atgtaccttt gttcttcatt gataaatatc
    35101 ctgaagcaaa atcgtgtact ccacctgcaa agattgttcc aaacacaatc cacaaaaatg
    35161 ctgatggtcc aaataacgcc ccttgtatag ctccaaatat cggacctaac cctgctatat
    35221 ttagaaactg aattaaaaag attctcttcc atttcattgg cacataatct actccatccc
    35281 tacaagctat agctggagtt tggtttttgt cattgattcc aaaaaccttt tctacaaaag
    35341 taccatagat aaaataacct actataagta ctactattga acctaaaaag cttaccataa
    35401 attcatcccc ttagttttta tataattgct ttataaattt ttatgagttt aagaaaacta
    35461 tttccagttc aaatagtaat acttttttat gctaatataa atattaatta cattaagtgc
    35521 atatttaagt acatgaaatg actttatata tgcattaaca gcctaaattc ttttactttg
    35581 cttctgctta cagttacttc atagtcacta ttttttaatt tcaatatgta tgtattgtta
    35641 aaccatggta taatttcttt tactttggtt aaatttacga tataagacct atgtgttcta
    35701 aaaaatgttt tatcatttat aagattctct acttctgata tgccttcttt taatataaac
    35761 ttttctttct cagtatatat aaatgtctgc ctttcattag cttcacaata atatatatca
    35821 tcaatatcta ttacaaccaa tttatcacct ttccaaaggc taattttatg tgttatttct
    35881 tctatctcct gatttactgt ctctttttta tatttatata aattactatt gctattagac
    35941 agttcaatat cactcatctc acttttttct aatttattca acatagaaat aattctctct
    36001 tcagaatatg gcttcagtac ataatcaaat acatctaaag aaaaagcatc tactgcataa
    36061 ttttcatacg ctgttataaa aacaatttta ggccttgatt taaactgatt taatgttttt
    36121 gcaagaagca ttccatcgag atgaggtata tttatatcca aaaaaattac atctatttta
    36181 ttttcctgaa taaagtctaa cacttctata ccattagtaa attcatttac tacctctatg
    36241 ctacttttat tctcaataaa atatcttaat tcctttcttg ccggaaattc atcttccact
    36301 attatagctc tcatacttct actcctttac ataaaatact atcaaagtcc cattgtctgt
    36361 tcttcttata ttaagccctc ttccatacat cagtttaagc cttaaatgta cattataaag
    36421 tccaatctta ttttctggca tattatcttg atatacatta tttataattt cctcacttat
    36481 acctatacca ttatcctcta ttgatattct cacagtattt tctaatgaat aaggtaactt
    36541 ttttatggat attttcacta caccagctct accactttct aaaataccat gtattatcgc
    36601 attttccact aatggttgta taatcaaact tggaatcttt atatttacat cttctatatc
    36661 ataagataca gttagtaatt ctccaaacct agctttttct atctctacaa aagatttaac
    36721 ttgttcaatc tctttattta tatcaattaa gttatcacta tattcaagat tatatcttag
    36781 ataagttgat aaatttatta ttaactctct agccttatct ggactaaatc ttataaatga
    36841 agctattgtg tttagggaat taaacaaaaa atgcggatta atttgtcttt gaagtgcttt
    36901 tatttctgct ttatttctcg cttctatatc ttttttatct ttctcaatcc cctctactat
    36961 agatataaca aatcctattc caatctgtcc aacaattaaa ggtaaatata tcccaacaat
    37021 tatttgaatt gctaaagaat gtggatatga tattaagtat ataagtagta tagtaaaact
    37081 ttcagaaatc atacctacca atataccata cattactcta tgttgctttg gtatcctctt
    37141 atatagaaat cctgataata cacctgctag tatacttgat aaaagacatg gaacagatgt
    37201 tatcccccca atatccataa aatatctatg aatccctgaa aaaactcctg ctgtaatacc
    37261 taccattgga ccaaataaaa tgccacctga tactatagat attattctag tgtttactat
    37321 tgaccccata taatttatac cactataagt tccaaatatg gctaaaaaag taaaaactaa
    37381 tgctatacag accaagtcat taaaactata ttcttccttt tgaaatatct gcttaaatat
    37441 ttttaacttt gttattagaa gaaacagtac tacaatgagg cttgtctttt ctattaatgc
    37501 gattaaaatt gatgttttca tattccacct ttaaaaaata aatttttaca cactctaaat
    37561 ttattatgtt tgttgtaact tatatatttg aagtaatcct atctcgacat tttccacatt
    37621 taacctttta attaaataaa aaatggatat acttctatta acaactaata ctagctgact
    37681 aacacaagct tgtaataact tgtagttatc attaactcta caattctagt atttaatttt
    37741 atactttaga aatctctttt cttagaagta atactttcga atattatata atacaatgtt
    37801 atgtagattc ttactcatac aacatatttc tttaaaaatt ttatactatt ctttgttcaa
    37861 atatctcatt tgatttaatt gtgctaaaca cacattatca tctctctgtt atatatttat
    37921 tatatagccg tatatcgata aagtaaataa atcctatatt taaaatcata tgtttgatag
    37981 taaatcatta taatgtatat ttaccataat aatttgccaa ccttgaaagt cacttctata
    38041 attagaagtt aaataattct tctaaaaaaa atacctctaa attttatttt ctgaaataca
    38101 tagttataac tatttttatc acgtataaga ataaaaattc cagaaaattt ttattatcta
    38161 agttaatata aaacatatta tttaatataa ataaattttt tataataata tattg
//
LOCUS       DAGQWR010000018        37877 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.018, whole genome shotgun sequence.
ACCESSION   DAGQWR010000018 DAGQWR010000000
VERSION     DAGQWR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.018"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(277..1020)
                     /locus_tag="MIW95_000824"
     CDS             complement(277..1020)
                     /locus_tag="MIW95_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBY3150172.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            1186..3753
                     /locus_tag="MIW95_000825"
     CDS             1186..3753
                     /locus_tag="MIW95_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3150173.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNNSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAVDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            3983..4387
                     /locus_tag="MIW95_000826"
     CDS             3983..4387
                     /locus_tag="MIW95_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY3150174.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4411..4908
                     /locus_tag="MIW95_000827"
     CDS             4411..4908
                     /locus_tag="MIW95_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3150175.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            4911..5312
                     /locus_tag="MIW95_000828"
     CDS             4911..5312
                     /locus_tag="MIW95_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBY3150176.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5330..5818
                     /locus_tag="MIW95_000829"
     CDS             5330..5818
                     /locus_tag="MIW95_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3150177.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            5845..6600
                     /locus_tag="MIW95_000830"
     CDS             5845..6600
                     /locus_tag="MIW95_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY3150178.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6600..7463
                     /locus_tag="MIW95_000831"
     CDS             6600..7463
                     /locus_tag="MIW95_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBY3150179.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKKKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7510..7989
                     /locus_tag="MIW95_000832"
     CDS             7510..7989
                     /locus_tag="MIW95_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBY3150180.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            8054..9361
                     /locus_tag="MIW95_000833"
     CDS             8054..9361
                     /locus_tag="MIW95_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY3150181.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETDMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9672..9812
                     /locus_tag="MIW95_000834"
     CDS             9672..9812
                     /locus_tag="MIW95_000834"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBY3150182.1"
                     /translation="MNGEGCILSNKITKECYKTGYMIREKSIDTYPDSGRGFMTWDEN
                     GE"
     gene            10324..11418
                     /locus_tag="MIW95_000835"
     CDS             10324..11418
                     /locus_tag="MIW95_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3150183.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQTQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSLLSENKEYKFLIEKLSLLEE"
     gene            11423..11623
                     /locus_tag="MIW95_000836"
     CDS             11423..11623
                     /locus_tag="MIW95_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBY3150184.1"
                     /translation="MENLMEYLPLLIPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            11623..12540
                     /locus_tag="MIW95_000837"
     CDS             11623..12540
                     /locus_tag="MIW95_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3150185.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGRTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIKIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNNEIQKFKSSGGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12537..13295
                     /locus_tag="MIW95_000838"
     CDS             12537..13295
                     /locus_tag="MIW95_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3150186.1"
                     /translation="MREYIAFTKKEFKENIRNYKLLSLTILFLVFGIMSPLSAKFMPD
                     LIAHFAPTLKVTTAPTALDSWTQFSGNISGLGTSLTLIVFCNILSNEYSKGTLVIMLT
                     KGLSRSSVVLSKFSIAVIIMTIGFWLSFLCTYGYTMYFWPTANLNHIIFSAFNLWLIS
                     IMYISILILGCVLFRPAFASVLLVLAATAILSLISIPKQIAPYTPNFILSKNIDLVSG
                     KVTPPEFIIPIIVTIVISIVCLLLAVVLFNKKAV"
     gene            13678..14751
                     /locus_tag="MIW95_000839"
     CDS             13678..14751
                     /locus_tag="MIW95_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBY3150187.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            14821..15084
                     /locus_tag="MIW95_000840"
     CDS             14821..15084
                     /locus_tag="MIW95_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150188.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            15290..16267
                     /gene="bioB"
                     /locus_tag="MIW95_000841"
     CDS             15290..16267
                     /gene="bioB"
                     /locus_tag="MIW95_000841"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBY3150189.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMVFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            16495..17508
                     /locus_tag="MIW95_000842"
     CDS             16495..17508
                     /locus_tag="MIW95_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBY3150190.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNSAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            17550..18440
                     /gene="rbsK"
                     /locus_tag="MIW95_000843"
     CDS             17550..18440
                     /gene="rbsK"
                     /locus_tag="MIW95_000843"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBY3150191.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIKVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKKLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            18488..19450
                     /locus_tag="MIW95_000844"
     CDS             18488..19450
                     /locus_tag="MIW95_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBY3150192.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            19504..21015
                     /locus_tag="MIW95_000845"
     CDS             19504..21015
                     /locus_tag="MIW95_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBY3150193.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            20999..21997
                     /locus_tag="MIW95_000846"
     CDS             20999..21997
                     /locus_tag="MIW95_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBY3150194.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            22181..23443
                     /locus_tag="MIW95_000847"
     CDS             22181..23443
                     /locus_tag="MIW95_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY3150195.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFQMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            23463..24206
                     /locus_tag="MIW95_000848"
     CDS             23463..24206
                     /locus_tag="MIW95_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBY3150196.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            24379..25764
                     /locus_tag="MIW95_000849"
     CDS             24379..25764
                     /locus_tag="MIW95_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY3150197.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFEYTPII"
     gene            complement(26101..26745)
                     /locus_tag="MIW95_000850"
     CDS             complement(26101..26745)
                     /locus_tag="MIW95_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY3150198.1"
                     /translation="MNNLDKNNVNEVLDMLLRTRRSIRSFTKEVPSKGLIEEIIETGR
                     LAPYAILSVAKQTDFRHFFVISNNSLQMNKIKTILVDVIKTQISVNEKKAKNNITLQS
                     FITSLKMQVEKGVLGIGTAPYLIIVAERRGIPPIEQKSLSHVMQNMWLKSTALGLGFE
                     LVSVIGRLSDNKDFCDILGVPTGEYGFDACAIGFPTTSVTREGRAIPHPSIKWI"
     gene            27009..27623
                     /locus_tag="MIW95_000851"
     CDS             27009..27623
                     /locus_tag="MIW95_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBY3150199.1"
                     /translation="MKKVSIDRRTIRTKKMIRSALAELIDEKGFNNISVTDLTQRADI
                     NRGTFYLHYVDKYDLLERIENEIIQEIEEETKHLDSSNMMEIDTSSEPLPFMVKLFEY
                     FRKNSVIIKSILGPKGDPTFERKIKKFIETNLFEKQKLINFNIDEAIISEEYFIQYIL
                     SADLGVIQYWLEKNMKESPEEMALILARMSLLGPLRAVGIRKDS"
     gene            27844..27984
                     /locus_tag="MIW95_000852"
     CDS             27844..27984
                     /locus_tag="MIW95_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150200.1"
                     /translation="MKNENISYLALGLCFGVVLGSLFKNLAIGLSFGLVIGVVLDSKK
                     NK"
     gene            complement(28214..28661)
                     /locus_tag="MIW95_000853"
                     /pseudo
     CDS             complement(28214..28661)
                     /locus_tag="MIW95_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            29431..30270
                     /locus_tag="MIW95_000854"
     CDS             29431..30270
                     /locus_tag="MIW95_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150201.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            30260..30703
                     /locus_tag="MIW95_000855"
     CDS             30260..30703
                     /locus_tag="MIW95_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150202.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            30714..31205
                     /locus_tag="MIW95_000856"
     CDS             30714..31205
                     /locus_tag="MIW95_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150203.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(31407..32135)
                     /locus_tag="MIW95_000857"
     CDS             complement(31407..32135)
                     /locus_tag="MIW95_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBY3150204.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            32260..33348
                     /locus_tag="MIW95_000858"
     CDS             32260..33348
                     /locus_tag="MIW95_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150205.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLIIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKELMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            complement(33786..34634)
                     /locus_tag="MIW95_000859"
     CDS             complement(33786..34634)
                     /locus_tag="MIW95_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150206.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMSNNMNSNMGMPNNMPNNMNSNM
                     GMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            34833..35198
                     /locus_tag="MIW95_000860"
     CDS             34833..35198
                     /locus_tag="MIW95_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3150207.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            35221..37608
                     /gene="cadA"
                     /locus_tag="MIW95_000861"
     CDS             35221..37608
                     /gene="cadA"
                     /locus_tag="MIW95_000861"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBY3150208.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLIVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFKEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELVPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGESSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKFNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
BASE COUNT        14734 a         3951 c         6814 g        12378 t
ORIGIN      
        1 attgacgatt attacaaata aaaataaagt aaatcttata taagatttac tttattttta
       61 gatgatattt ttttagcttt atactataag gtatataaaa ctaagcctat taaaaatgtt
      121 ataatttcta ttaccatcat agaccatatc aatccatcaa gttcataaaa gtaattaagc
      181 tttattaaat agcttcttta ttataatttc tttatttcat ataatataaa tagtattaaa
      241 atttttatgg gagttaaagt aagcatgtat tattacttag atatttgtat aatcaacatc
      301 tagtaattta cctttttggt gaaaatgatt aataagtttt ttaggataat tcattattaa
      361 ttcatctgga aaattaattt cctttaagat attctcagaa tactcatagt tacctatgtc
      421 aaaacaaatg tgagcatctg aagttagtat gattttagca ccatattttt tgcaaagtgt
      481 agcaatatac ttacagttat ccgagctacc atttcttgac acgcctttaa tagaacaatt
      541 atttatttct attaagatat tcttttctat agctaattta atgattaatt catagtcgat
      601 tgggtagttt gggtttccaa ggtgtcctaa tatttcaata ttatcatgct tatttattgc
      661 atttattaaa gctttagtat tattctctaa tgtatttgga gaaaaaacag cttcgtgaaa
      721 tgacaagata agataatcta accgaggaat tacaaaaggc tctaggtcta tgtttccttt
      781 aatatctagt atatttgctt cacagcctct taaaattata atgttattta tgattcttgg
      841 gatattttga atgttgtgaa agtaccatct gtggggagaa ccaggcatct taggaccatg
      901 ttcagatgta cctagtatct taattccatt ttctttacaa taattaatat tttctagtaa
      961 agtgctatag ccatgaccac tgactataga gtgggtgtgt aaatctgata aaaaattcat
     1021 ataaattcct cctctataaa agatgagggt aacgtaattt atattataaa acgaatagga
     1081 aagaaaatca atagttacat aattagacaa aaatattaaa atattgtaga attaagctta
     1141 ataataaatg tttaacagta ttactgctta taggggaggt taactatgac acaaaagaag
     1201 ctatcaagga aagaaaaagt tttaatttgc ttgaaaaggt taacagaaga acacttgaac
     1261 tttaataatg gcattagatt tggatttgat gcagaatata tagctactga agtaggtata
     1321 aatagaagta atgctagtaa ggaattaaat caattaataa aagaaagtat tattataaag
     1381 ataaagggta agcctgttca gtacttatat aaagaaagta ttgagagtat tttgggaaag
     1441 aaactaaata aagggatatt tgataatatt aactctataa acgaatatag tgaaaatgat
     1501 tctatcaaag atgagtatat tttagagagt ataaaaaaag agaacaatat ttttaattta
     1561 gtaggatgtg atggaagttt aaggtcccaa atagaacagg ctaaagcagc agtaatatat
     1621 ccacctaagg ggttgagtac attaataata ggacctacag gagttggtaa atctatattt
     1681 gcagaatata tgtataagta tagtttaact ataaaagaag atagtgataa tgcaccattt
     1741 atcatattta actgtgctga ttattctgat aatcagcaat tattattagc gcagcttttt
     1801 ggatatgtaa aaggagcatt tactggagct gataaagata agtatggtat tgtacatgaa
     1861 gctaataatg gagtattgtt tttagatgaa gttcatagat tatctgcaga gggacaagaa
     1921 atgctatttc tgcttatgga taaagggata tatagaaggt taggagaggc taataaggta
     1981 cacgagtgta gagttatgat aattgcagcc actacagaag acccagaaac agcaatgttg
     2041 gaaacgtttt taagaagaat acctgtaact ataaagattc cgtctttaga ggaaagaggc
     2101 tttcaagaga gaatgaagct tatatgttat ttttttaaag aagagagtgt taggattggt
     2161 gttaagttaa aggtttcaaa agaagttatt aaggcattta ttttatataa gtgtaaggga
     2221 aacataggcc aattaaaaag cgatatacaa ttaatttgtg caagagcttt tttagattat
     2281 atgacctata aaagggaatg tgtatatgta aggctctctc ttctaccaaa taatattaga
     2341 gaatcattat atggaaataa tagaaaagag gagtttgtac aaaatttcag ttttattgga
     2401 aaagatgata ttattttttt accagaagaa aaggattttg aaagtgaaaa tttattaata
     2461 gaaaataaaa aatatgattt agatttttat ggaatgataa aagaaacttg gaataaactt
     2521 caacaagaag gtattaaaga gtcacaaatt agaagtgtta tagatggaga catacagaag
     2581 tattcctata atttaatgaa tacttttata tataataata gtaatcaaac agcttataac
     2641 aacattgtta atgattcaat tgcaactact gtaaaatata ttttacaaaa gcatgataaa
     2701 tggagcaaaa gagaaggatt agataaatta ataaaagcta tagccttaca tattcagaat
     2761 ttaattgaaa gaataaagat tggaaatata gtaaagcatc ctaataaaga tgaaattatg
     2821 aaggaaagag catatgaata taacatagca aaagaaatat tatctaacat gtcattaatg
     2881 tatggagtag agtttccaga agatgaagca atttttttag caacatttct ttatttatcg
     2941 tatgtgggat taaatgagga gagtatagca atattagtca taatgcatgg tgaatctaca
     3001 gcatctagca tggtgaatgt agccaatact ttactggatt gtaaccatgc agtggcaatt
     3061 aatatgggat tagaagatag agttcaggat attttagtac aagctgtaga aatagccaat
     3121 aaaattgata aaggaaaggg aattttaatt ttaactgata tggggtctat tttaacattt
     3181 gctaaagtta ttcgtgatgc tacaggaaaa gaagtaaaag cagtagatat ggtaagtaca
     3241 cctattgtaa tagaagcaac tagaaaggct ctaactccag aaatgacatt agagaaatta
     3301 tattttaata ttatagaatc tataaagaaa cattatggag atatggaagt tacatatgct
     3361 gaaaaagata atggtggtag atattttgat agactactaa ttgataatat tagtaaaact
     3421 ttgacttttt taaatgggga aaaggcttat tttatattaa aagaagtaat taatagaatt
     3481 agtgaacact attctttagt aattcaagat gaacttttag taaagtttat atttcattgt
     3541 tcatgtatga ttgaaagagt aataataaaa gagtctttag tttataaatc atatgaagaa
     3601 agaattagta gaaataaaga attatattta gttataaaag aaagttttaa gctagtagaa
     3661 gagacattcg atataattat ttcagatatg gaatatgcaa atatattaga tatatttgaa
     3721 tcacaatatg atacagataa attcaaaagt tgatacaaaa tttaaaaata gccaaaaggc
     3781 tatctttttt ttactttttt tgatacagat ttgatacagg ttgaaaaaat cttttgatac
     3841 atatcgaaat aataagtttt taaaaaaaat tgagtaaaaa gttttgatta aatcctgtaa
     3901 aatagccatt tattattgat acacataagt tggcacggca attgcttata taaaaagtga
     3961 caagaaaagt gaggtgaaat tggtgatagg aataatagtt atgactcatg ggagttttag
     4021 tgaagaaatt ataaaaagct gtgaacttat tgcaggacca gcagaaagaa cagcagcaat
     4081 taaactaaat aggaatgata atattgaaga cttaaataaa aattttgtag aaaagttaaa
     4141 tgaattagat gaaggtgacg gagttttagt attagctgac ctattaggag gttcgccaag
     4201 taatgttgca agtttaaacc ttaaaaaagg tggcaagttt catgcactta ctggagtaaa
     4261 tcttccaatg ctacttgaag ctttaattaa tagagaggga aaaagtttag aagagttagc
     4321 agaagcatgt attgaagcag gacaaactgg tataaacaat attaataaag ttcttagctc
     4381 aatgtaatag aaaaaggagt ttgattaaaa atgtctttag taaaatattg cagatgtgat
     4441 gatagattaa tacatggtca agtgatatat aaatgggtta agcatctagg tgtaaaaaag
     4501 atagtagtag tagatgatga aacaaccaat gatgttattg ctaaaggttt gataaaaatg
     4561 gcagcaccaa agaatattga tttatcaata ttaactgtaa gtgaatcaag gagatatttt
     4621 tataataatc aagctgatga taacgttttt gtattaatta aaaatttaga tactgcaaat
     4681 agaatgatag aagaagggat taatatcaaa aaacttattg ttggaagaat acctacagga
     4741 ataggaaaaa agaaaatatc tcaaaatgta tacattaata aaaaagagtt tcttttaata
     4801 gatgaattta ttaaaaagaa tattaatgta tcaattcaaa tggtacctga tgaagaagaa
     4861 gtagaattaa accaaaatat taatcgctat aaaggagagt ttatttagca atgaataaat
     4921 ataaggtaat tatactgtgc catggaactt ggggaaaagt cttagtagaa gaaacaaata
     4981 aaaactttgg attgtcaaat caatatgaag ttttatcact aagttctgaa aaagatgttt
     5041 ctgtatttat gaaagaggtt gaagaagtag tagataaaga agatgtgaaa attataatat
     5101 cagatttata tggaggatct acatcaagtg ttgcaatagc tgttgctata agaagaggaa
     5161 tagatgcaat aaatggactt agcttacaga caattttaat cgtagatgaa gaattaagca
     5221 aagaagataa attacataca ttaccagaaa gaatcgtcaa aaaaaataat aacctttgtg
     5281 tggatttaat taaagaattt aaaaaaatat aaattatgag gtgaatataa tggcaaaaat
     5341 aacattagta agagtagacc acagattaat tcatgggcaa gttataacaa aatgggtaaa
     5401 aatcgctcaa gctcaaaaaa taataatagt tgatgacttc ttaggtcaag atgaatttat
     5461 ggctgacata tataaaatgg cagcaccatc aggagtagaa gtagttattt taacagcaga
     5521 agatgcggga caagcattcc aaaacaacac tttaggagat aaaaatatat ttattctatt
     5581 taaaaatgtt gatatggcaa ataaggctta taaagctgga cttaaatatg aaaagattca
     5641 attaggaggt attccaaatg aagcaggaaa gaaaatggtg tttacagctg tttcattagg
     5701 aaatgaggat gtagaacaac ttaatgaact taatgaaaat ggggttgaaa tagtcctaca
     5761 agttatacca gaggaatcat ctatgacata tgaaaatgcg ttaaagaaat ttaaataaaa
     5821 aatataaatt aggaggaatt ataaatggaa tctgttttga tgttggcaat tgtaacagga
     5881 ttatggtatt ggtttgcagc aggtcttgca gggtataccc ttttctcaac actaaagtct
     5941 cctttattta taggttttag ccttggatta ctttggggtg atgtaacaac tggtatgatt
     6001 gttggtgcaa gtatagaaat ggtttatcta ggaatggtcg cagcaggggg aaatattcca
     6061 tcagataaat gtctagctgc tcttattgca ataccagtag cacttcaaac tggtgttaat
     6121 gcagaagttg cagtatctat tgcggtacct ttaggtgtta taggagtatt agttaataac
     6181 ttaagaagaa ctggtaatgc tgtattggtt cataaggcag ataagtatgc tgaagaaggt
     6241 aatacaaaag gcatctggag atgtgcaaca ctttattctt taatatttgg gtttgtttta
     6301 agatttccaa tagtttttgt atgtaacttt tttggagcag atttagttca atcattatta
     6361 gatgttatac cacaatggtt aatgaatgga ttaacagtaa tgggcggtat attacctgca
     6421 ttaggttttg caacaacaat atttactata ggtaaaaata aattcttacc aatgtttatt
     6481 ataggatttt ttatggttca atattttgaa atatcaatta ctgcagctgc aatatttggt
     6541 gtttgtatag ctcttttaat tacctttatg aaagaagaca aaagggtagg tgaagtataa
     6601 tgcaagaaat aaaaaataaa gaagttaaaa atggacaagg tccaactgaa gttaataaga
     6661 caagagtact cacaaagaaa gatgtaacaa aaacatacct aagatggtgg tggacagcag
     6721 agttatctaa ttcttttgaa agaatgcaag cattagcagt ttgtgcaagt ttcactccgg
     6781 ctttagaaaa gttatataag aaaaaagaag atttagtaga tgcattaaag agacacttac
     6841 aattttttaa tactcaagct atatggggag gattaattca tggtacagtt ttagctatgg
     6901 aagaagagaa agctactgaa ggaaaaatcc caggagaagt tatttcaggt gttaagaatg
     6961 gtcttatggg acctttggct ggaattggag atactttaga ttttggtaca tttcaaacaa
     7021 tattcttagc tttaggtgct tcatttggag cagaaggaag tgttataggt gcatttttcc
     7081 caataatgtt tagtatatta ctattttgcg aaggttatta cttatttcat ttaggatatt
     7141 ctttaggtag agattcaatt aagaaaattc tatcaggtgg tattgtcaat aagataattg
     7201 atggtgcttc aattctagga atgtttatga tgggagcatt atctgcaact acagttaagc
     7261 tttcaactcc gctatcattt gatattggtg gtaaagcaat tgttgttcaa gatacattaa
     7321 atatgattgc tccaggttta ttaccacttg gtgttgtatt cttcgtatat tggggaatga
     7381 aatacaagaa gtggacaata actaagttat tagtaatttt agttgtatta gcattggtag
     7441 gatcattcat tggaatattc taattcacag tgattaaaat atagaattta attttaggag
     7501 gagaaagaaa tgtcagaaaa aatgtataca gaaaaggaat atttagaagg aattagagga
     7561 gcaacaggag aaagagcagt atggttctat cttcttatga aagaagctga aaaattagga
     7621 gctaatccag atgatatatg taaagaagca atatatggat ttggtaaaat gagaggtcaa
     7681 aaatataacg tagcagatac tccaggaaaa atggcagaaa tgttatataa cagtaaagga
     7741 caaaaagtgt ttgaaatgga attagtagaa aatacagatg agaatggcgt tttaaaattc
     7801 catcactgtc cattagatgc tgcttggaaa gaatatggat taactaaaga agaaagaaaa
     7861 gaaatttgta gattagcttg ttatggggat tatggaagag ttgactgtgc acaaggtgtt
     7921 aaattagatt ttgctcaaaa atgtgctcat gatgatgaag tttgtgaatt agtatttaca
     7981 aaaaaataat ttttaatgct aaaagcctgt tctttaagga caggctttaa aaagatagaa
     8041 agtaggttga tttatggata taagcagaag tgtagataat agtttaaata gaaccataga
     8101 atttctaaaa gaattgataa aaattgattc acaacaaggt gaacctattt cacaatgtcc
     8161 ttttggtata ggacctaaaa aatctttaga taaaacttta gattactgtg ctagtttagg
     8221 ttttagtgtt aaaaatattg ataattatat tggatatgca gaaattggag agggagagga
     8281 attaataggg attccaatgc atttagatat agttccacca ggagaggggt ggagtgtaga
     8341 cccattttcg ggagcagtaa ttgacaatat tatttatgga agaggtgtca tagataataa
     8401 gggtgctgta tctatgctta tacatgtatt gaaaaacata gaggatatgt atcctacaat
     8461 taataaaaga ataagattaa tttttggtac aaatgaggag acagatatga aatgtatcaa
     8521 atattactta gataaaggtg aagaaattcc aagtatggga tttactccag atgctatgta
     8581 tccagtggtt aatggagaaa aaggaagggt tcatataagg atagaaaaag aaattaaaat
     8641 tgacaaaagt aaaccttaca taatagtaag gggaggaact aaagaaaatg ttgttccatc
     8701 tcattgtact gcaaaaatta ttaatggaat tatttcagag ttaactacta aaggagtagc
     8761 tgtacatgca agtaatcctg aaaagggaga aaatgctata tctaaaatgg taataaagat
     8821 agttgaggat aatatggatt ttcagcatag agaagatata gaattagttt caaaatacct
     8881 ttgtagtgat tattatggag atgcattggg gattaatcaa tatgatgagg tattcaaaaa
     8941 cactacctta aatttaggaa tattaaaagt aaatgaggaa aaaatcgtat gtgagttaga
     9001 tataaggtat ggtaaaaata tagttttaaa taacataata gatagattca aaaaggtttt
     9061 ttgtaatggt tggaaaatag aggttattgc tcataaagac ttacattatg tggatgagag
     9121 taatttagta cttaaaaaat tattagaagc ctatgaagaa gtaacagatg aaaatggata
     9181 tacaattgct atgggtggtg gcacatatgc aagttggttt aaagatatgg tggcgtttgg
     9241 acctaagttc ttagcgtata aaactggtgg tcatggagta gatgaaagag taccaataaa
     9301 tcatatacga aagaatatgg agatatatac tttggcttta ataaaactat tagaattgta
     9361 aaagaagaag agactttatg tgcaaattta cagatatctt aaaaagagag ataaaacgag
     9421 ttcaacttta tttaaatgtt caacaccatc aaatggggct gtctcaaaat agagataagc
     9481 ctcttgctta ttattgaaat gatgaaaata tattttactt catacaaaaa agagtgcctt
     9541 atgaagtaaa aagttaattt tgagacaccc tcatttgatg atgttgaaga gaaaataagt
     9601 atgaaattat taaaaaaaga tgattttata aaagttaagg ttgaaaataa aagttaattg
     9661 aatggaatta gataaatgga gaaggctgta tactatctaa taaaataaca aaagagtgtt
     9721 ataaaactgg ttatatgatt agagaaaagt ctattgatac ttatccagat agtggacggg
     9781 gatttatgac ttgggatgaa aatggagaat aataaaaatt atagtttata caatttaata
     9841 agatatatgt acaagcagtt aatctaaatg attgactgct tttcttttat agatttttat
     9901 ataatagaca attactaata catgatagtt gcctgttata attagaggaa tagatacgaa
     9961 taataacttt ataaaagaat taaatggaaa attgaagagt tatattcagg attttaaaaa
    10021 gaatatgaaa tgagaaatga aaataaggtg tttatattaa aagaggtttt acacaaaata
    10081 cttaaaaacg tgataaataa aaactgtatt tagataaatt aactactagt tttatatata
    10141 atatggattt tgtttacaaa gtttacatat ttcacaaaat atttttaaaa tatatttaaa
    10201 aatatatttt atatacttgt tcgtttgttt ctctgtggtt gattttttcg tcaaaagtca
    10261 acttcaaatc gcttgttagc tttacatgtt taaggatata attatacata aggagggatt
    10321 gacatgaatg aattaaacat cgcaaaaacg ctgattttaa agcgtaaaga aaaaggaatt
    10381 acacaagatg aacttgcaaa ttatataggc gtttctaaag cttctgtatc taaatgggaa
    10441 actggacaaa gctatcctga cataaccttt ttaccgcaac tcgcgacata tttcaacatt
    10501 accgttgatg agttaatatg ttatgaacct caaatgatga aggaagatat taataaactg
    10561 tataataaac tgtgcaaaga ctttacggtt aaaccgtttg atgaagtcat ggatgaaata
    10621 agagaaatca taaaaaaata ttattcatgt tttcctttga tttttcggat gggtctttta
    10681 atagttaatc actatgatat tgtagatgaa aaaaagcgtg aactattaat tagtgaagca
    10741 ttagaaatat ttattcgtat acaggaaact tgtaatgaca tagatatttg ccgtcaagca
    10801 aaaagtatgg aggcaacttg ttatatattg ttaaatcaac ccatacaagt gattgattta
    10861 ttgcaaaaca gcaattttcc catgataaat gaatctatac ttcttgcaca ggggcaaatg
    10921 atgaatggtc aaatggatga agcaagagaa acatttcaac ttggtgcata tcagaacctt
    10981 atatcattag tccaaaatct agtgggtatt ttgcaaaatg cagataaatt acaaatgaag
    11041 gaaatagaaa gacgtattct agcaatttca gatatatttg agcttgatac attatctcct
    11101 gctattatgc tttcttcata tttaacacag acacaaatta atttaataca tggggataat
    11161 gaaggagcta ttaaatcatt acgaaaatat gtagatttag caacacgtga tatataccca
    11221 attataatac atggggatga cttttttaat aaacttgaca gatggatttc tgaaattgga
    11281 acaggtatta cacgtgatga tacaatcgta aaagcaggta ttgttgctgc tattaaaaat
    11341 aatccaatgt tttctttatt aagtgaaaac aaagaataca aatttcttat agaaaaatta
    11401 tctttattgg aggaataaga ttatggaaaa tttgatggaa tatttaccac ttttgattcc
    11461 tgttattatt ttagatttaa tacttataat tactgcactt gttcatgtat tgagacatcc
    11521 aaattataaa attggaaata aggcaatatg gattattgta gtactgttta ttagtctaat
    11581 aggaccaatc ttgtatttta caataggaag aggtgaagaa tgatgaatat tgtctcaata
    11641 aatggattat caaaaggttt tggtaatcga aagataatag ataatctaaa ttttactgta
    11701 ccagaagggt cagtctttgg gtttgtaggt aagaatggtg caggtaagac tacaacaatg
    11761 aaaatggtac ttgggttact tgaaccagat agtggaacta tagatgtttg tggtgagaaa
    11821 gtgacatatg gaagaacaag ttctaatcgt catgtaggtt atctgcctga cgttccagag
    11881 ttttacaact atatgagacc acttgaatat ctttctcttt gtggtgaaat tacggggctt
    11941 tcaaaaaaag aaataaagat aaggagtgaa gagttgttat cacttgtggg gcttggcaat
    12001 gaaaagagac gaataggtgg gttttctcgt ggaatgaagc aacgacttgg tatagctcaa
    12061 gctttgcttt cacgtcccaa attacttatt tgtgatgaac caacaagtgc acttgaccct
    12121 gttggtcgaa aagaaatatt agatattatg cttaaaatca aagactcaac aactgtaata
    12181 ttttcaacac atatactatc agatgttgaa agaatctgtg accatgttgc tatactaaac
    12241 aaaggaagca ttgctcttag cggtacactt tcagaaataa aaagtatgca cggaaaggat
    12301 agacttttac tggagtttgc aagtaataat gaaattcaaa agtttaagtc tagtggtggc
    12361 attaaatcct tactaaaaga ttcagaagaa actaatatgg aaattgtttt gcatggtaaa
    12421 gatataaaag caatacaaaa aacagttata tctacactcg cagaaatgaa tctttgtcca
    12481 gtaaagatgg aactaataga attgtcactt gaaaatttgt ttttggaggt agtaaagtga
    12541 gagaatacat agcatttaca aaaaaagagt ttaaggagaa cataagaaat tataaattac
    12601 ttagtttgac tattttattt ttagtatttg gaataatgag tcctctttct gcaaaattta
    12661 tgccagactt gattgctcat tttgctccaa ctttaaaagt tactactgca ccaactgctt
    12721 tagattcttg gacacagttt tctggaaata tttctggtct tggaacgagt ttaactctaa
    12781 ttgttttttg caatatttta tcaaacgaat attctaaagg aacactcgtt attatgttga
    12841 ctaaagggtt atcacgttca tctgtagtat tgtctaaatt ttctattgca gtaattatta
    12901 tgacaattgg tttttggttg agctttttat gtacttatgg atatacaatg tatttttggc
    12961 caactgcaaa tcttaatcat attatatttt cagcatttaa cttatggtta attagtatta
    13021 tgtatatttc catattaatt cttgggtgtg tactatttag accagcattt gcaagtgtgt
    13081 tacttgtatt agcagcaact gctattttga gtttgatttc tattcctaaa cagattgcac
    13141 cttatacgcc taatttcata ttatcaaaaa atatagattt agtttcaggt aaagtgacac
    13201 ctccagaatt tattattcca atcattgtaa caattgttat ttcgatagta tgtctgttat
    13261 tagctgttgt tttgtttaat aaaaaagcag tttaaggata tttttattat aaatacaaat
    13321 agtgctgtat tgtgggaaaa tgtgtttagt attgtagaag aatatattta agtaaaaaag
    13381 aggctgtctc aagatagaga aataagcctc ttttttattt tagtcctcct cactatactt
    13441 tataataatt aagttattac cttaaggaac tatctaaaaa aagagatata gattataatc
    13501 taatgcttat cagttatttg aggtgtttag tatatagagt ctaaagtagt gaaggactaa
    13561 aataatattg ttttcattaa ataaagataa gaatctttaa ttgtggtaaa atatactaaa
    13621 aaatcatttt aaaagtgata aaattatttt aagtaaatat tttaaagggg tgtagatatg
    13681 gaaaagtcaa aagtatattt ttgtgattta tactcaaatt cgcaaaataa aaacgtacca
    13741 aacaatgtaa gaagattatt tgatgaagca ggattcaaag atttaataga aaagaatgac
    13801 caagtcgcaa ttaaacttca ctttggagaa aagggaaata ctacatatat gagtcctgtg
    13861 gcagttagac aagtggttga taaggttaaa gactgcgaag gaaagccatt tttaacagat
    13921 acaaatacac tgtatacagg gagtagaaca aattctgtag accatcttac aactgctata
    13981 gaaaatggat ttgcttatgc agttgtaaat gcaccagtaa taattgcaga tggattatat
    14041 agtagaaatt atgagaatgt aaaaatagac aaaaaacatt ttgaaagtgt aaaaataggt
    14101 ggagaaattt ataattcttc tgcaatgata gtaatgagtc attttaaagg tcatgaagca
    14161 gcaggttttg gaggagcatt aaaaaactta gctatgggat gtgcaagtgc agcaggaaaa
    14221 caaatgcaac attctgatgt tactccagta gtaaaggaaa agaagtgtgt aggttgtgga
    14281 aagtgtgtta attcttgtcc tacaaaagct ataagtatag tggataaaaa agcagttata
    14341 gattctgatg tatgttatgg atgtggagaa tgtccaactg tctgtccaac tagagcagta
    14401 actatacaat gggaaagtga ctcagatgtg tttgtcgaaa agatggcaga atacgcttat
    14461 ggagcagtat caaataaaaa agacaaggta gggtatataa cttttgttat gaatgtaact
    14521 ccactttgtg attgtgtacc ttggtcaggt agaccaattg cccatgatat aggaatatta
    14581 gcatcaacag accctgtagc tattgaacaa gcatgctatg atttgatttg taaggaaatg
    14641 ggtcatgatg ttttcaaaca tgaacatcct catgtaaatg gaactagaat aatagactat
    14701 gcatgtgaaa tgggtatggg aagtaaagaa tatgaattaa taaaattata agaatagtag
    14761 cacttaaatg catataatta ttgtaagaga aatctaaaag tatcaaagga gtatgcattt
    14821 atgaaaattt actatgagaa ggaaaacagc aagaaggcta atgtaaacaa tattccaagt
    14881 gtatttgcgt ttaattttct aagaaacttt ttgactagca aagagtatga ggaaactaga
    14941 aaagaatatt ttataaataa tctaacaaaa agtcaggtta atcaagccat gaaggattta
    15001 aaatggctat ttagagaata tgaaggctta gaggttatta caatggaaag cttggatgga
    15061 gttaagacaa ggatagtttt ataaaccagt aagaattata ttctaataaa aaatactgct
    15121 ttaataataa tatgtataat ctatatacta atattatttt gaagcagtat ttttttgtgt
    15181 atagtaaact tgtgtctaaa ataaagttga ccaataaatg ttatatatgc aatactatat
    15241 ataataagta aactacataa aaatattaag ttgacaaata ggaggatata tggaaaaata
    15301 cataataaaa ttaaaaaata aagtattgag agaaaaagaa ataagttatg aagaagctct
    15361 taatttaata agccttgata caaataataa aaacgatttt gatattctat taaaatctgc
    15421 aaatgaaata agagaatatt ttatgggaag aaaagcagat ttatgtacga taatgaatgc
    15481 taaatctggc aaatgtagtg aagactgtaa attttgtgct caatcatctc attataaaac
    15541 tggtgtagaa gaatattcat tattagatta caatgaaatc ttaaatagag ctaaagagat
    15601 ggagagtaaa ggagtgcata gattctcatt ggttacaagt ggtaaaggta tgtcaggaaa
    15661 ggagtttaat aatattttaa atatctatga aggattgagg gaaaatacta atttaaaatt
    15721 atgtgcatct ttaggtatta tagattatga gcaagctaaa atgttgaagt cagcaggtgt
    15781 gactacatat caccacaatg ttgagacgtg tagagataat tttcataata tatgtacaac
    15841 acacacatat aaagatagga taaaaactat aaaagatgct aaaaaagctg ggctagatgt
    15901 atgtgtaggc ggtataattg gaatgaatga gagtgaagaa cagagactaa aaatggtttt
    15961 tgaaatacga gaattaaatg taaaatcatt tccaataaac attttaaatc ctattaagaa
    16021 tacgccaatg gaaaattatg atgtattaga accaatggaa atactaaaaa caacagcagt
    16081 atttaggttt ataattccta atgtgtacat aaggtatgct ggtggaaggc tatcattaaa
    16141 gggatatgac aaagttggat ttaatggggg agtaaattct gcaattgttg gtgattattt
    16201 gacaactgtt ggaagtggga ttgaaaatga taaaaaaatg attatagagc aaggatttga
    16261 actttaataa atactactta aattaattaa tatactttta gataattata aaagtatatt
    16321 aatttttttt gcagattaat ttaaatatta aaattgaatt ataagttgca atattttgaa
    16381 aaaagggaaa aatatcaaaa tttatataaa atggttgcaa atgggaaaag gatgtgatag
    16441 tatctaggta accggttaag taaaacggtt tcctaaattt tgggggtgaa cttcttgagt
    16501 ataactataa aagaaattgg cgaattggct ggtgtttcta aaacaactgt gtcaaaagtc
    16561 attaataata aagatgaaaa tataagtcag gcaacaagag aaaaaatttt gaaaataatg
    16621 aaggaaaaaa attatgtacc aaataaatta gcacaaagtt tagtaactaa aaaaactaat
    16681 actatagggc ttcttatacc agatatacga aatccattct ttacagatgt atcaagaggt
    16741 gtagaagaca aagctaatga agaaggctac aatataattt tatgtaatac agatgaagat
    16801 gctaaaaaag aatatgaagg aataagaaca ctatcagaga ggatgataga tggaattata
    16861 tttgcagcat cttcaaatac aaactggaaa gaagcaaatt ataaagatat aaaaatacca
    16921 actgtcttga ttgataagaa gataagtatg aacaaagaaa ccttaaaagg aattgttaaa
    16981 ataaataact ttgaaggggc ctatatagct acaaaacatt tattagatat tggaaataag
    17041 aaaataatat acctaagtgg tccacttcaa aatgaaatag cagtagatag attagaagga
    17101 tataaaaagg ctctaatcga gtgtaattta agctacaatt ctgcttatgt atttgaagga
    17161 aaatataaga ttgaatgggg acaagaattt ataaaaaatt tagaaaagat agattttgat
    17221 gcaatatttt gtgcaaatga tttgattgca ataggtgtca taagaggtct taaagaaaga
    17281 gggttaagta taccaaatga tgtaagtgtc gtgggatttg atgatattca aacatcaagt
    17341 ctaataagcc cttctcttac cacagtaaaa cagccgtctt atgatatagg atataaagcc
    17401 agtgaaatat tgataaattg tttaagagga gataaaaaag aatcatttga tgaattgatt
    17461 ttcaaaccag aattagtcat aagagattca acaaaagaaa gtgaataaca gcacgagaga
    17521 cataaataga tatgtaaagg agtaatgtaa tgggaaacat agttgtcata ggaagtgtaa
    17581 acatggacat ggtatgttct gtagataaaa gaccagaaaa aggagaaaca gtattaggta
    17641 atagtttttt tacatcacct ggagggaaag gggctaatca agctatctca gcttctaaac
    17701 taggagcaaa tgtaaaaatg atatcatgca taggggaaga tggcttagga gaggagttaa
    17761 taagaaattt tagaacgaat aaagttgatt atagtttagt atccagaaat aatcacaaaa
    17821 gttctggtgt tgctgttata acattatgtg aaaatgataa tagtattgtt gttgtaccag
    17881 ggacgaatga gctagtagat atagaattaa ttaaaaagaa tgaagaagag ataaagaatg
    17941 cagatatagt attgctacaa ttagagattc cattaaaaac aataaattat gtagtgaatt
    18001 tctgttttga aaataggatt aaggttttat taaatccagc accagcagta aaactaaatg
    18061 aagatataat agaaaaagta acttacttaa caccaaatga acatgaatat aagatagttt
    18121 ttgacacaaa tgaagggata gaagaagtat taaaaaaata tccaaataaa cttgtaataa
    18181 cagaaggaaa aaatggagct agattttatg atggtgagga aatcaagcat gtatcttgta
    18241 taagtgttga tgttcaagat accacaggag caggagatac atttaatgga gcattggcag
    18301 tggctataac agaagggaaa aaattatata cagcagtaga atatgcagta gtagtatcag
    18361 gtctttctgt aactaaacta ggtgcacaat ctggtatgcc ttataaagaa gatgttgaaa
    18421 aatatttaaa taataaataa aaatctaatt tgttcaaatt taaaactatt aaaaatggag
    18481 gttaattatg agagtgagaa aaattttatc tttaggaatt gctggaatac tagcaatagg
    18541 aatgttaaca gggtgttcaa tggaaggacc aagtaaatca gataataaag gtggttcaga
    18601 caagaaagat ttaactattg gagtatctac aataactctt caacatcaat tttttataga
    18661 tattgatgag ggaataaaag aaaaagctaa ggaattaggt gtgaaagtta tagtaaatga
    18721 cccagaccaa gatgtagcta agcaaacatc agcaatagaa gattttatac aacaaaatgt
    18781 agatggaatg atagtacttg gaactgataa ttcggctata gtaccagcag tagaaggagc
    18841 atttgaaaaa atgccagtag ttacagtaga tgctgtatta aatactgaaa atattacaag
    18901 ttatgtagga acagttagtt atgatgctgg taaaaaatta ggggaataca ctaaaaaata
    18961 catagatgaa aagttaggtg gaaagtcaga aatagcaata gtgacagatt taaaatctca
    19021 aatacaaatg cagagaatag atggatttaa agatgcgtta aaaggttcag caaatgttaa
    19081 gatattaaat tcacaaccag gatatgacag agaagaatct cttaatacag tagaaaactt
    19141 aattcaatct aatccagatg tagatataat atatgcaaca gctgaaaata gtgtactagg
    19201 agctaaagct gcacttgaat ctgcaaaaaa taaggatgtt aagattgtag gatttgactt
    19261 aactgaagaa gcatcaagtg gaataacaga tggaactatt cttgctatga ttcaacaaca
    19321 acctaaagag atgggacgtt tagctgtaga agcagtagta aaagctataa aaggggaaaa
    19381 ggtagaaaag aatataccag taccagcttt actttatgat aaggaaaata taaaagactt
    19441 taagaattag taaaataaaa tgagattatt tgaaagaaat cctatttaaa aaggagtgtc
    19501 attatgagta atataattct gaagttaagt aatattgcta aagaatttcc tggtgtcaga
    19561 gcattagata atgttaattt tgagttattt catggcgaag ttcatgcact actgggtgaa
    19621 aatggtgctg gtaaatcaac aatgataaag attttaactg gtgcacattc gaaaacttct
    19681 ggaaaattta tttttgaagg caaagaaata gagaatatat ccccagatat ctctaaaaag
    19741 ataggtataa atgccatata tcaagaacta acagtttttg acgaattaac agtagcacaa
    19801 aatattttta tgggaaaaga aataaatggc aaagtcttaa ccaatgacaa aaaaatgaat
    19861 gaagaggcta aaaagatttt cgataatatg ggaatagata taaatccaaa tagtttagtc
    19921 aaagagctta gtatagctca aaaacaaatg gtagaaatag caagagtttt atcatcagaa
    19981 actaaagtat taataatgga tgaacctact tcttcaataa gtaaaaaaga gactgaaata
    20041 ctttttagac ttataaatga tttaaaagag agtggagtta gtataatata tatatcccat
    20101 aggatggaag aactctttga gatttgtgac agaataacta taatgcgaga tgggaaaacg
    20161 atttcgactt tgaataccaa agatgtatcg tctgaggaag aattagttaa tttaatgata
    20221 gatagaaagc tagaccaatt tttcccaaaa agaaaagtag aaataaaaga agaaattatg
    20281 cgagtggaaa atctaactaa gaacaatgtt tttaatgata ttagctttaa tataagaaaa
    20341 ggtgaaatat tgggaatagg tggactagtt ggaagtaaga gaagtgaaat agtagaagct
    20401 atatttggac ttagaacata tgattctggg aaaatatatt taaacaatga agaagtaaag
    20461 tttaaaactc catcagatgc aatagaaaat ggactgggat taataacaga agatagaaaa
    20521 ggtactggat tgtttttaca aatgagtgtc aaagaaaaca caactatggc tgggttaaaa
    20581 aagatatcta agtttaaatc aattatagat agaaaaaagg aaaaagagat tttagaaaaa
    20641 tacatagaag ctttaaagat aaaaacacca catatgaatc aagttataca aagtttaagt
    20701 ggtggaaacc aacaaaaagc tataatagca agatggcttt tattacaacc agatatattg
    20761 attatggatg aaccaaccag aggtatagat gtaaatgcta aggcagagat atacaatcta
    20821 atgggagatt tagtggagag tggtgtaagt ataataatga tatcttctga aataccagaa
    20881 ttaatatcaa tgagtgacag aataatggtt atgagagaag gtcatataag tggattttta
    20941 gaaggtgaag aaatggttga aaataatgta ttaaaattgg ctttcggagg taaaattaat
    21001 gagttcaata actaagcata aagaagaaaa atcttctcca ttattgacaa aattaacaag
    21061 tgtatttaga gaccaaggga gtgcagctgt aggacttgta ataattttta taataatgag
    21121 tgtagcttca tctaactttt taactttaga taatttaatt aatgttggaa gacaaatatc
    21181 aataaatgca atacttgcag taggtatgac atttgtaata attacaggag gaatagattt
    21241 aagtgtagga gcagtaattg ctttagtagg tacattttgg gctacaacag tagtgaatta
    21301 taatgcacct atatgggtag gtatgatact tgcattagca atagggacaa tattaggagt
    21361 cataaaaggt gcaattattt caacacaaaa actacctcca ttcattgtaa ctttagccat
    21421 gttaacgata ataagtgggg caagctttgt atttacagga ggaagaccta tatcagtaaa
    21481 tacagatgca tttaagatgc tcggtagagg atatattgga ccaataccaa taccagttat
    21541 aattatgatt atagttgtaa tagcaggaca ttttctatta aaaagaacta attttggacg
    21601 tcatgtacat gcagtaggtg gaaatgaaga ggccgctaga ttatgtggag taaaagtaaa
    21661 taaagttata gtaaaagtat acgcactagc tggactacta acagcattag ctgggataat
    21721 actatcatca cgtttggcat caggttcacc aactgttgga gatggagcag aattggatgc
    21781 tatagcagcc gttgtgcttg gtggtactaa tatgatgggt ggttctggtt ctatagtagg
    21841 tacatgtata ggtgtcggaa taataggaat attaggaaat ggattaaact tattaagtgt
    21901 atcttcatat aatcaaatga ttataaaagg tttggtaatg ttatttgcta tatggataaa
    21961 taatataaaa ttaaaaaaat cagctaaatc taaatagtaa ttaaatttta gcttataaac
    22021 tataaaaatt attaagtttt tgtatatatt tttatacttt aatttgcata catctttata
    22081 cattgaatgg gaatatgcta agtgaaaaag tttactatgg cttaaataga tgagattaga
    22141 cttgaaattt taaatgtaaa aataaatctt aggaggaaat atgaaaaaaa ttctcttaga
    22201 tacattaaat agtaaaaaac aagaatacat agattattta aaagaacttg taagtataaa
    22261 gactgaagat gttggtcatg gaatactagg tggatttgaa aaagaaggac aggaatatat
    22321 tgaaaaactt gcaaattaca taggttttag tgtagataga caagaaatga gcgaagaatt
    22381 aattaaaaaa gcaaaaaata tatataaaga aggaaatcta ggtcacaatt atcaagatag
    22441 atataattta atttgtaagt atagtgatga tttacctggt aagacaattg tatttaatgg
    22501 tcatgtagac actatgccac caggtgatat aagtaagtgg aaatataatc catatcgtgc
    22561 tacagaagat aatggaaaac tttatggtct tggaactgct gatatgaaat ctggacttat
    22621 agcatcaata ttagctgtaa agcttataaa agatagtggt ctaaatgtac ctggtaatgt
    22681 aaaaataatg tcagtagtag atgaagaagg tggcggaaat ggtaccataa atgcagttat
    22741 gaatggtatt ggtggagatt gttgcataat ttgtgaacca tcagaacaaa acttaatagt
    22801 tgcacatatg ggatttgtat tctttgaagt tgaggttaaa ggtgtgtctc ttcactgtgg
    22861 tagtaagtgg gagggtgtaa atgcaattga gaaggctatg ttgttgcttc aagatataaa
    22921 agagttggaa cataactggc ttatgatata taagcatcca ctattacctt cacctacaat
    22981 aaatttaggt gtaataaatg gtggaacagc aggttctact gtgccagata agtgtgtatt
    23041 taatttatgt gtacacttct tgccaaatat aatgagttat gaacaagttg taaatgatgt
    23101 aacaaatgtc ataatgacaa gggctaatgg agatttatgg cttaaagata ataaaccaaa
    23161 tataaacata tatcaatcag ggttaggttt tcagatggac aaggattctg attttgtagt
    23221 gaatgcacat aaaatactag aagaaacttt aggaaaaaaa ttagagataa aaggttcaac
    23281 agctggcaat gatgcgagat taatgaaaaa tttagctgaa ataccaacac ttatattagg
    23341 gccaggttca atagaacaat gtcattcaat tgatgaatat gttgaaatta aggagtattt
    23401 agatagtata cttatgtatg caagtctaat attaaactta taaaagtagg aggaatatat
    23461 aaatgaaaaa agtattaata gtaggggaat cttgggtaaa aaatataact catataaaag
    23521 gatttgatac gtttgttaca actcattatg aagaagctgt aaagtggttg aaagaagcaa
    23581 tagaatctgg aggatatgag acagttcata tgcctgcaca tgtagcagca gatagttttc
    23641 cgtataaatt agaagagtta aatgagtatg actgtataat attatctgat ataggttcta
    23701 atacgttctt attatctaat agtactttta tagattgcaa tagtaatcca gatagacttg
    23761 aactaatcaa agagtatgta aataatggtg gggcgttaat catggtaggt ggatatatgt
    23821 catttacagg aatagatgca aaagctagat ttggagaaac tgctatcaaa gatgtattac
    23881 caataacaat gatagataaa gatgatagag ttgaaaaacc agcaggaata attccagaag
    23941 ttattgactc tgaacatcca gtattaaaag gtataccaac agaatggcca aagtttttag
    24001 gatataacaa aacagtagct agagataatt gtcctgtttt agcaactatt ggaggagacc
    24061 catttgtagc tgttggcgag tttggaaaag gaaaatctgc tatctttagt tctgactgtg
    24121 cacctcactg gggtccaaaa gaatttactg attggaaata ctataataaa ttatgggtta
    24181 atatgcttga ttggttaaca tgctaaaaat tttataaaac ttgaaaacct cttacttgat
    24241 aaaatgaaac ttacaagtca agaggtttta ttgtataaaa tggttgtatg gtagaaaata
    24301 tttttaaatc agttaaaatt agtcattttg gctgataatc gataagttat tagaaacagg
    24361 gaggtttata taattaatat ggataattta aataaaaagt ttttgatgga atatatagat
    24421 gagatacagg gagaattaag agatatatca gataagatat ggaaaaatcc agaacttcag
    24481 tataaagaat actatgcttc aaatttacaa aaagaatgtt taaaaaaaca tggttttagt
    24541 atagagggat tagaaggtat ggatacagca tttgtggcta gttatggaga aggtagacca
    24601 gttatagcag ttttgggaga atatgatgct ttagatggtt tgtctcaaaa agtatccact
    24661 actaaagaac ctattgtaga tggtggtgca ggtcatggat gtggacataa cttacttggt
    24721 acaggttcaa taggagctgt aattgcagta aaggaattaa tcaaactaaa aaagctgaaa
    24781 gggactataa aatactatgg atgtccagca gaagaagatt tatctggaaa agtattgatg
    24841 ataaaaaaag gtttttttga tggaattgat tgtgctttta gttggcatcc atttgatata
    24901 aatactccga taagaatccc aacacttgct aattactctg tgaaatttag atataatggg
    24961 attagtgctc atgcagcaca agctccttat aacggaagaa gtgcacttga tgcagtagaa
    25021 cttatgaata taggttgtaa ttatttgaga gaacatatat ttgataattg tagagtacac
    25081 tatgtaacta ctaatggtgg aaaaatgcca aatatagttc ctgattttgc agaagtgtgg
    25141 tattacataa gaggagttaa aatggagcat gtaagagatg tatttggtag gattgttgat
    25201 atagcaaaag gagcagcatt aatgactggt actattatgg aatatgacat tataagtgga
    25261 gtatatgact atattccaaa tacaatatta acagatattc tatcacaaaa tatgaaatta
    25321 gtaggtgtgc aaaactataa tgaagatgac tactattttg cagataaatt agctgagaca
    25381 gtgtctatag ataaaagaag tagtgtttct agtgtattaa gtggaaataa ggatattact
    25441 aagatgaatt tacatgatga ggttacagat gatacattta ctcataataa ctgtatatca
    25501 ttatcattgg atattggaga tgttagctac ataattccta cagcacaatg ttcttgttca
    25561 gtttggccta ttggtatttc tgcccataca tggcaatctt gtgccagtgc aggttctgat
    25621 atgggattta aggcaatgtt attggcatct aaatctatat catgttcaat aaatgatgta
    25681 atgcttgatg aattggttat aaataaagca aaaaaagaat tgaaggatac tgtaggtagt
    25741 tttgaatata ctccaattat ttgaagagta atttaataca agtaagattg aattgtatga
    25801 attaggatga atatataaat taatattatt tttaacttaa ctttctaatt attttcaatc
    25861 taagatacaa taattagaat atatttttaa agtttcctat ttaaggaaaa taatttgatt
    25921 ttatgtttga taaaaggact tataatttgt aagccctttt attatattta ttttttgttt
    25981 ataattaatt ttatttttta ggtttagtta ggttatagat taccctgaaa aataacattt
    26041 aacttattat taaaaacatg taagaatagt ttataaatat tttattttcg atgttgttta
    26101 ttatatccat tttatcgatg gatgaggaat ggctctgcct tccctagtta cagatgtagt
    26161 agggaaacca attgcacagg catcaaatcc atattcacct gttggtactc ctaaaatatc
    26221 acaaaaatct ttattatcgc tcaatcttcc tattacacta acaagttcaa aacctaatcc
    26281 taaagcagtt gatttaagcc acatattctg catcacatga gataatgact tttgttctat
    26341 aggtggtatt ccacgacgtt ctgccacaat aataagataa ggtgctgttc ctataccaag
    26401 aacccctttt tcaacttgca tttttaagga agtaatgaag gattgaaggg taatgttgtt
    26461 ctttgccttc ttctcattga ctgatatttg agttttaata acatcaacaa gaatggtttt
    26521 tattttattc atttgtaaac tgttatttga aataacaaag aaatgtctga aatctgtttg
    26581 ttttgctact gaaagtattg cataaggtgc aagtcttcca gtttcaatta tttcttcaat
    26641 cagccctttt gaaggtactt cttttgtaaa acttcttatg gatcttctag ttcttaatag
    26701 catatccaaa acttcattaa cattgttttt atctaaattg ttcattttta tcctccacta
    26761 atagtattct taagatgttc tataatgcat ccatataaag gcgaactttt cggtcatatt
    26821 aaacatttcc ttcaaattaa gtcgctattt ctatgaacac aatgttgatt aataatctat
    26881 atgtatatta taatatttat attgcattat gcaataaaca ataatattta aactgttaag
    26941 taataaacat ttactataaa attgttaatt aaaattaaaa aaatttatat ggaatgagga
    27001 aataattcat gaaaaaagta tctattgata gaagaactat acgtactaaa aaaatgatta
    27061 ggtctgcatt agcagagtta atagatgaga aaggatttaa caatatatca gttactgatt
    27121 taactcaaag ggcagatata aatagaggaa ccttctatct gcattatgtt gataagtatg
    27181 acttgttaga gaggattgaa aatgaaataa tacaggaaat tgaagaggag accaaacatc
    27241 ttgattcatc caacatgatg gagatagata cctcaagtga acctcttcca tttatggtaa
    27301 agctttttga atattttaga aaaaattcag taattattaa atctatcttg ggtcccaaag
    27361 gtgaccctac ttttgaaaga aaaataaaaa aatttattga aaccaatttg tttgagaaac
    27421 aaaaactaat aaattttaac atagatgagg caataatttc agaagaatat tttatacaat
    27481 atatactttc agcagactta ggtgtaattc aatattggct tgaaaaaaat atgaaagagt
    27541 cccctgaaga aatggcactg atacttgcta gaatgtcgtt actcggtccg ttaagagcag
    27601 tgggaattcg taaagattca taaaaatagt tttatatata ttagactttg aaaatatatg
    27661 tttaaaaacc attttttact actactgttt ataaattagg ggagatatct taaaatatta
    27721 gtaaaaggag tgctaagtga gtgtaataac tatgagataa gaggtttagt tgatatagtc
    27781 aaaacttcaa aagaatcaat tagaaaagtt aaaatattgc aattaaattt aaaggagtaa
    27841 tatatgaaaa atgaaaatat aagttatttg gcgttaggat tatgttttgg tgttgttttg
    27901 ggttcattat ttaaaaactt agctattggt ttatcctttg gattagttat tggagtagta
    27961 ttagacagta agaaaaataa ataaaacttc aatagtaaca tataacaatt atactatttt
    28021 tgtataaaat cattggaaat caacaataga aaatatttgt tatttaaaaa ctaaaaatac
    28081 agtttaaacg acctaaaaat atattatata attattataa caagataagt gagagtaagg
    28141 ttatgataat agagtaatat ttttcaatta ttactctatt tatatttatt tagttatact
    28201 atagtttata ttgttatttt attttcatat gcagtatagg gaatggatta ccttgttcat
    28261 cgaactcaga ttttttaaag actttaaatc ccatatgttt ataaaagcct aatgcttgtt
    28321 gattttgctc attcacatct acataattta catttaatac ttctattgca tattctacaa
    28381 gtctttttcc aattccattt cctatactat aattacttac aaataacatt tcaattttaa
    28441 aatcatgtat tcccataaaa gcttttataa tacctttttt tgtctcttac acataaaaaa
    28501 ttactaacat atttagcaca ttctttaact tgaggcttta ttgatattat attttcttca
    28561 cttaaaaagt catgtgttgc tcttactgat gattcccgtg tatctagtat ttctttcata
    28621 tctttattgt taataatatt taaattttca atttttatca tttaataatt cctcttaagt
    28681 aagtatgtta cttaatatta tatgttaaat atgatttaaa aaggttataa gaatattttt
    28741 gtattaaata taatcaaata aatatattta tttgtcataa attaaatgtc cactaaaata
    28801 gaaaaaataa gaagaaatag gtaataaatg atagcttata gcaaaataaa gatgtaagta
    28861 taacaataga tgagattgga attaagtcac tatacctaac agaacattct taggcgaata
    28921 tagaagactt aaaaaagaaa agagaagtag acaagaattt attaatgatt acatataaaa
    28981 taaatgaatt ataattttat taatgatata ataataatta acaaaaaata aaagaggaac
    29041 gagttaatgt ttgatggcat acactcattt tctaaccaat aaaacaatag attcaattaa
    29101 ggttactaaa gtctaatcaa cacataaaca tataggataa atacatcttt ttaatatatt
    29161 aattatgtta gacttacaaa aaatgtaaat acatagcttg ttaaatcttg attgaacctt
    29221 aagtaataag ggataaaaaa tgacttgttc aaaggcaaag aatatactat taaatgatat
    29281 aagtaactta gagataatat gtagtaagtc aaaagaacta tatagcatca gtaagtaaaa
    29341 agcgtaaaaa cttacataat gcactataaa ataaatgagt tagaaaataa atataattta
    29401 tataataaag tagtacaaaa aggagtattt atgtttggat atgttaaaat aaacaaaatg
    29461 gatttaacat ttagagaata tgattattac aaagcttact attgtggact atgtaaatac
    29521 ttaaaaagaa atcatggaga aatctctagg ttttctttga attatgatat aacattttta
    29581 attgttttat taacagcagt atataaccca gagtctataa gtacagagga agtgtgtata
    29641 gtaaatccat ttaaaaagaa aaaggtaatt acaaatgata ttacagaata tgcagcaagt
    29701 atgaatattc ttttaacgta ttataaattg gaagataatt taatggatga taaacgcata
    29761 aaagataaat tggcatatta tatatataaa aacaaattaa aattagcata tgaaaagtat
    29821 cctgaaaaag cagagtatat aaaacaacaa ctaaatgaat taaataagtt agaaaaagat
    29881 aaaaatataa atatagatga agtatctagt atatttggaa atataatggg tgaagtattt
    29941 gtctataaaa aagatgaaaa tgagagaaac ttaagaatga taggttttaa tataggtaag
    30001 tatatatatc tactagatgc ctatgaagat ttagatgaag attttaagaa aggaagatat
    30061 aatccattta tagagtatat tgataaaaat gatgagttaa aagaaaaagt aaaaaaaata
    30121 atagtaacat cattaggatt tttagctagg ggaattgata atctaaatct aaataaaaat
    30181 gttggaataa tagaaaatat aatttattct ggtgtttatt taagatatat taatatatta
    30241 gaaagtagag gtggaaagaa tgtacagtaa tacttataat aatgaaaatt ttatgagagc
    30301 aagagcattc atagaaaata gtgaatttaa aaaagcatat gattttttaa aaactttgac
    30361 tgacaaatgt gcagagtggt attatttaac tggcttttct gcaatgaaca taggatatta
    30421 tgaagaaggt gaagacttct taaaaagagc aaaatttatg gagcctgaaa atagtgaata
    30481 tagtgatgca cttagaagtt atacacaata tagaaatgat tatagcaata gggctgacaa
    30541 ttataataga agaagacgta atgatttaga tggatgttgt tgctgttgct gtgatgattg
    30601 ctgttgttgt ctaggtgacg attgttgtga aaattgtgct aagctttggt gtttagattc
    30661 atgttgtgaa tgttttggtg gagatttaat aacttgttgt taggatgata aatatgagta
    30721 taaaagttgc ttatggagga gttttattag cattaaatgt gatattgttg actcttacaa
    30781 atattattcc tgtaaataca ttgtttatta tggggttagc atcactttta gtatcaattg
    30841 taataatgga atggggcttt aagagtggaa tcgcttttta tataggttct atagttttag
    30901 gatttatagt gatggcaagt aagtctcaat ggattgtata ttcacttacc tttggaatat
    30961 atggtattgt aaaatatctt atagaaaaag ataggtctat atatattgag tattttatga
    31021 aattagtatt tgcaaatata atgatactta tattgtattt tttattaaga acaatagttt
    31081 atattccaat aaacatattt attataggtt catttgagat agcctttata gtatatgact
    31141 atgtttacag ttcttttata ggttattaca ataataggtt aagaaagatg ctatttaaaa
    31201 aataatagtt gatttttata tttaatttta atgaaattgt agtttggata agtgtaaatg
    31261 atatatctga atatacatta aagcataaag ctaaatttaa tatattctta gtgcaacata
    31321 taaaagcttt gatttttagt caaagctttt atatgttaga tataaatatt agatataata
    31381 taagttttta aaatagatag tataaattaa atatccacta tatttacatc tgtagataga
    31441 gaataaataa tatcttttct ttctactatt atctcaacat ttttagaaat ttcctcatca
    31501 agtgatattt gagataaaag ttcctttgtt ggatttattg ctattggatt tccaactctt
    31561 tttagcatat ttatatcacc attagtatct ccataggcat acgagtttga taaatctaaa
    31621 tcatacttta caacaaaatc atttatagca ttattcttac ttatagaatc ccacattgga
    31681 ataactgtgc ctgtaaatat attattttca aagacataat cacttcctag aaaatcggtt
    31741 acattatatt tttttgccat tttttcaaca agaaatccag ggctaccaga tataaatata
    31801 actatgtgtc cttgatttag atgccactca atccttgaac gtgtgtattt ataaactcta
    31861 tctgctttta atttaattac ttggtcactt gtaaagttga tacaggtttt atctaatcct
    31921 tttagtgaat caacataaag gtcacatatt tctaatagat aatcatcata attaccttgt
    31981 cttttatccc aatccataaa agtgtctcta gcatgtttat accaattttt ttggtcaatt
    32041 atatcgtatt taattaattt tttaaaatgt tcaaccataa gagagtctct ataaagagta
    32101 ccatctatat caaaaaaagc tgctatattt ttcataagac cacctcaatt aatcatttcc
    32161 ttatattata ttataaattg tatctaagtt gttaaaatat atgtataata atttatatat
    32221 taaaaaacaa gtattttaga gtttaaagga tgtgactata tgaattttaa ttttgcaaag
    32281 gctacaaatt ctagacttat gggcagttta ggtcttataa taaattggat agatgatgaa
    32341 aacaatcact tttgtcaata ttttttatta gatgcagagg gattaggatt agcagattat
    32401 gtgagtttaa ataacccaac acaagaagaa gcttatatgg aagaagaaag attgatggga
    32461 ggatttggtt cagatagagt tgaattaacg aaggatgaat ctttattttt agtatcttat
    32521 tttggtaata agaatcttta ttatgataag ctattgccag gagataaatg tgagtatatt
    32581 gatattataa aaaactataa aacagattta attatagaaa aattatacaa taaaatatgt
    32641 aaaagagttg atgaggaagt tgaatttata aactatatga ctatgagatt tatagcatgg
    32701 gatagagaaa gtttaaaata tttttctgga agtgacgaga ttgcaaatat gcatattact
    32761 aatataaatg gaacattact taaaaacgtg gtaagtgata aaggtcaagg cagatatata
    32821 agtgaagctt tatatgaaga caatgatgga tattatatat gtaaaattgc tttttgcata
    32881 agtaagtgct atgaaactgg ttttagaatt aattctctat tagtgacaga taaagaactt
    32941 atgtatgatt ttgaggtatt tgatgagatt tctaaaagtg aatttgtaag tgtttacagt
    33001 gtaaattcac ctgaagaatt tgcaggagta ttttatagag aaaatccatt tctgttaaaa
    33061 agtaatatga atgaaggtat attttttact agatttaatt ttaataatga ccatgtaaaa
    33121 gaaaatgtat atataataaa caatgatatg aaagctatat attacttaat gggaaataag
    33181 ttttttattg gtacttataa cgaaaatgat tgcaattata tcaatgaaat aagtcagtct
    33241 aattatagtg actatattaa atttgaagag aaatttttct ttgagcaaaa tgcattatat
    33301 gactttgcag aatcaggttc attagatttt gatgattttt tggaataata aatttagaca
    33361 ttatttaagc gagattaact gaatttaatc tcgcttaaat ttatttctat aatattgggg
    33421 gaatattatg aggaatacat ttattattgt ggtcaattat taaaatttta tacagtttat
    33481 tataaaaaat ttatactttt ctttatgttg tttgccaata ttagtctcaa atacgagtac
    33541 catactattc tgacccacca acacaagaaa gagtttttta aatgaaaaag tcagataaaa
    33601 ttaaatttta tctgactttt tctatgttta tcttattaga ttatgttaat atctgttgac
    33661 cgaataagcg catactcagt caactttcta tacctataat aatattgggg gaatattatg
    33721 aggaatacat ttattattat ggtcaattat taatgtttta tacaacttgc tcaaaaaaat
    33781 atatattaca tttcttcctc atcaaattct tcaatattag tagcctgtgg tataccttgt
    33841 atagacatat tactagaacc ataaggagta ggatatatat taggaggcat agtactaggc
    33901 ataactacag gcataccttg cattgtatta tgacatataa ctcctggtat catcataagt
    33961 acattaggag gcatattttg attacatgag aagttaggaa tacccatagt attattcata
    34021 ttatacccca tattactgtt catgttatta ggcacattgt taggcatacc catgttacta
    34081 ttcatattat taggcatatt gttaggcata cccatgttac tattcatatt attaggcata
    34141 ttgttaggca tacccatgtt actattcatg ttattaggca tattgttagg catacccata
    34201 ttactattca tattgttaga catattgtta ggcataccca tgttactatt catgttatta
    34261 ggcatattgt taggcatacc catattacta ttcatattgt tagacatatt attaggcata
    34321 cccgtattat tattcatgtt attaggcata ttatttgtag cagatgtatt gtaattcata
    34381 tttccggatg tattatttgt gttaggcaag ttgtttggag aattactact tttaggataa
    34441 tgatttacat tattaggata taggttattt atgttctggt tattaggtat ttttgtataa
    34501 ccattagaaa tttgagggtc atttctcaat tcatcttggg gaatttcttc attagtaata
    34561 taatctgcat agttcgctgg gtctaagtaa ggcatgtaac tagtatctaa atcctttttt
    34621 ttcttgttac tcatattaaa cttgcacctc ctagaaaact ataaaaaagt ttacaaaaac
    34681 ttattctaat aatatatata ttaaaaaagt ttataatatg tgcattctaa ttatattaaa
    34741 ttaataaaaa ttattgttgc aaatttagga atgatagcat ataatgaaca tatgaacagt
    34801 tattcatata ttaaaacaat ggaggattaa agatgagaga agagataaat gattgtaatt
    34861 gtaatatagt acatgaagaa atagtaacag aggctaaatc aactatgcca gatgaagaga
    34921 tgttatatga tttagcagaa ttatttaaag tatttgggga tactactaga gtaaagatat
    34981 tatatgcatt atttgcaaat gaaatgtgtg tatgtgatat agcgagttta ttaaatatga
    35041 ctcactctgc aatatctcat caacttagag tactaaagca ggctagatta gtaaagttta
    35101 gaagagaagg aaaaacagtg tattactcat tagatgatag tcatataagc cagatatttg
    35161 attgtggatt aaatcatatt agagaaactt ataaatgata aatgagagga gtgaatatat
    35221 atggaagcaa ataatttaat taaaaaagaa tttatactag gaggattaaa ctgtgcacat
    35281 tgtgcagagg agattaataa taaagtttct aaactacaag aagttaaatc ttccaactta
    35341 aattttataa ataaaaaact tatagtaaat ataaaggaaa gctttaatga ggatactact
    35401 atagaaaaga taatagatat aatagattca acagaaccag gtcttgatat acaaataagt
    35461 tctaaagaaa atgcatctag taaaacatca attaaaaaag agcttatatt aggaggatta
    35521 aactgtgcac attgtgcaga ggagattaat aacaaggttt ctaaattaaa agaagttgaa
    35581 tcttctaatt taaactttgt aaataaaaaa cttacagtaa atataagtaa taactttaaa
    35641 gaggatgatg ttataaataa aataaaagaa ataataaatt caacagagcc aggacttgat
    35701 atacaagtag ggtctactga caaagtaaaa gggagaacta ctgaaaaatc aggagctgta
    35761 aatgatacaa ataagaaaga attagttcca cttataattg gagcacttgt atatatattt
    35821 ggtatatatc aaacagctac aggttatgaa agtcagttta gcaatatagt atttatagtt
    35881 gcctatgtaa tagtaggtgg cgatgtgctt ttaagagcaa taagaaatat atcaaaagga
    35941 agagtatttg atgagaactt tttgatggct ctagcaacag taggagcatt aacaataggg
    36001 gagtcatcag aagcagtagg ggttatgctt ttctataaag taggagagta tttacaaggt
    36061 gttgcagttg gtaaatccag aaagtctata acttcactta tgcaaataag accagattat
    36121 gcaaatctaa aatttaattc tgaagttaag gtagtgtcac cagaagaagt aaatgtaggg
    36181 gatataatag tagtaaaacc tggtgaaaaa gtaccattag atggtgtagt agtggatgga
    36241 gtttctatgc tagatacttc tgcactcaca ggagagtcag tgctgagaga agttgaaaaa
    36301 ggagatgaaa tcttatcagg tgttataaat aaaaatgctt tattgagtat tgaagttaca
    36361 aagagttttg gagaatcaac agtttctaag attcttgacc ttgttgaaaa ttctagcatt
    36421 aaaaaatcaa agacagaaaa ctttatatct aagttttcaa gatactatac tccaatagta
    36481 gtaatagctg ctttattaat agcatttgtg ccaccacttg taatctcagg tgaggtattt
    36541 agtgattggt tatatagagg attgatattc ttagtcgttt cttgcccttg tgcattagtt
    36601 ttatcaatac cacttagttt ctttagtgga attgggtttg catctaaaaa tggtatcctt
    36661 attaaaggaa gtaactattt agaagcttta agaagtgtag atacagtagt atttgataaa
    36721 acaggaacac ttactaaagg tgtgttcaat gtaactaagt taaatcctga aggtatatca
    36781 gaagaagaat tattagaata tgcagctatt gcggaggtaa attcaaatca tccaatagca
    36841 aagtctatat taagttacta taataaaaaa attgatttag atacaataga cagttatgag
    36901 gaaatagcag cttatggaat tagagtaaag cataatggca attttatatt agcaggtaat
    36961 gagaaactta tgaaaaaaga aaatatatct tattcatcag ctaaagaagt aggcacagta
    37021 gtctatattg ctgttgataa agtatataga ggttatatag ttatatctga tgaagtaaaa
    37081 gaagatagta aaaatgccat aagaagctta aaagcaatag gggtaaaaga agttgtaatg
    37141 ttaactggag ataatgagaa agtagcaaaa aatattgctc aagaattaga attggataca
    37201 gtttactcaa atttacttcc taatgaaaag gtagatagat tagaagattt atatgaagga
    37261 agaactgaaa aagagaaaat agcatttgta ggtgatggaa taaatgatgc tccagtatta
    37321 gctcgtgctg atgttggtat agctatggga ggcttaggtt cagatgctgc tattgaagct
    37381 gcagatgtag tgcttatgac agatgagcct agcaagatat ctaaagcaat tgaaatagca
    37441 aacaagacta ataagatagt gtggcaaaac ataatatttg ctctaggagt aaaaattata
    37501 gttatgatac taggtgctgg aggagtagct actatgtggg aagctatatt tgctgatgta
    37561 ggtgtggcac ttatagcagt agtaaatgct atgagagcta tgagataagg gataaatgta
    37621 aatccatact atttaaaaaa cttataatta tatattgaag tatgtaataa tgaaaggtat
    37681 caataagttt cttttaactt tattggtacc tttttattgg agcgctttgc atatataaat
    37741 ttatattcat tatccattgt gtttatttaa tgtatttatt tagtgtagag cttttatatt
    37801 gtaattttaa aatttagaca tagaaggttt attaataagt ctatattaag tttaaattag
    37861 attcataaaa attataa
//
LOCUS       DAGQWR010000019        37066 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.019, whole genome shotgun sequence.
ACCESSION   DAGQWR010000019 DAGQWR010000000
VERSION     DAGQWR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.019"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..393
                     /locus_tag="MIW95_000862"
     CDS             <1..393
                     /locus_tag="MIW95_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY3150209.1"
                     /translation="SICKKYDIQIEDNINISKLNFIENNDICSIFANSLDNAIEACMD
                     INNELEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQTSKDDDKIHGIGLASIKY
                     IVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            405..545
                     /locus_tag="MIW95_000863"
     CDS             405..545
                     /locus_tag="MIW95_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBY3150210.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            604..1185
                     /locus_tag="MIW95_000864"
     CDS             604..1185
                     /locus_tag="MIW95_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBY3150211.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNISVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNNDQ"
     gene            1262..3040
                     /locus_tag="MIW95_000865"
     CDS             1262..3040
                     /locus_tag="MIW95_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150212.1"
                     /translation="MNLLNIVESIVSCIKGLARRGINMSSIKVISDFITKIIRKNILF
                     AITVIIINYHLIQRWTHISNRGISFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTV
                     KILCRNNFDYFLGISVAVTRNNFIFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILVFE
                     WFLPLTILSIVISFISVIINNLILKITLSSVFLYLTSTKMLDRVIDIKEPFFKALFKS
                     LNIFEDQSYAYYSDFVGKVNNSAYVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLI
                     VIVVYSILFIGLNYISIDMSTYRDEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNI
                     KDRIVLLNTSNEELTNINMILDEIFDIKYIKVNSENIKFNKKKDLIEVNLKESLEPND
                     EVTLDIEYEGYINVLTSWNQNKYIATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSK
                     NNVYSNLPLLNKTSNFLKKDYIFEGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMD
                     IEEKIKFLIETRNEILGDSKASVDAKKYVDEVDNVMESIKRKKINMILVADLPSNEFL
                     YEDSKGEKISITIYHWLSGNILIIDK"
     gene            3055..3927
                     /locus_tag="MIW95_000866"
     CDS             3055..3927
                     /locus_tag="MIW95_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY3150213.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYIPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINNKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            3928..4716
                     /locus_tag="MIW95_000867"
     CDS             3928..4716
                     /locus_tag="MIW95_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150214.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYLGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVFYIARKSTVLKKSK"
     gene            complement(4812..5645)
                     /locus_tag="MIW95_000868"
     CDS             complement(4812..5645)
                     /locus_tag="MIW95_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBY3150215.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPLGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(5713..6342)
                     /locus_tag="MIW95_000869"
     CDS             complement(5713..6342)
                     /locus_tag="MIW95_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBY3150216.1"
                     /translation="MDENITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMI
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            6653..7312
                     /gene="cprR"
                     /locus_tag="MIW95_000870"
     CDS             6653..7312
                     /gene="cprR"
                     /locus_tag="MIW95_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBY3150217.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            7523..8581
                     /locus_tag="MIW95_000871"
     CDS             7523..8581
                     /locus_tag="MIW95_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBY3150218.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            8832..10976
                     /gene="fdhF"
                     /locus_tag="MIW95_000872"
     CDS             8832..10976
                     /gene="fdhF"
                     /locus_tag="MIW95_000872"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:9246..9248,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBY3150219.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKTWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            11025..11858
                     /gene="fdhD"
                     /locus_tag="MIW95_000873"
     CDS             11025..11858
                     /gene="fdhD"
                     /locus_tag="MIW95_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBY3150220.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCASVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            11869..12042
                     /locus_tag="MIW95_000874"
     CDS             11869..12042
                     /locus_tag="MIW95_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150221.1"
                     /translation="MCTKKEEQKCSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            12100..12651
                     /locus_tag="MIW95_000875"
     CDS             12100..12651
                     /locus_tag="MIW95_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY3150222.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            12676..14052
                     /locus_tag="MIW95_000876"
     CDS             12676..14052
                     /locus_tag="MIW95_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBY3150223.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            14052..14567
                     /locus_tag="MIW95_000877"
     CDS             14052..14567
                     /locus_tag="MIW95_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY3150224.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(14645..15928)
                     /locus_tag="MIW95_000878"
     CDS             complement(14645..15928)
                     /locus_tag="MIW95_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBY3150225.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSHSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            16116..16538
                     /locus_tag="MIW95_000879"
     CDS             16116..16538
                     /locus_tag="MIW95_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBY3150226.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSEKNGENIIRDILDGNISSECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            16640..17578
                     /locus_tag="MIW95_000880"
     CDS             16640..17578
                     /locus_tag="MIW95_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBY3150227.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            17764..19788
                     /gene="ligA"
                     /locus_tag="MIW95_000881"
     CDS             17764..19788
                     /gene="ligA"
                     /locus_tag="MIW95_000881"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBY3150228.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            19948..21297
                     /gene="rph"
                     /locus_tag="MIW95_000882"
     CDS             19948..21297
                     /gene="rph"
                     /locus_tag="MIW95_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBY3150229.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARAIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            21297..21767
                     /locus_tag="MIW95_000883"
     CDS             21297..21767
                     /locus_tag="MIW95_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY3150230.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            21961..23247
                     /locus_tag="MIW95_000884"
     CDS             21961..23247
                     /locus_tag="MIW95_000884"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBY3150231.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            23396..23980
                     /gene="clpP"
                     /locus_tag="MIW95_000885"
     CDS             23396..23980
                     /gene="clpP"
                     /locus_tag="MIW95_000885"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBY3150232.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            23999..25249
                     /gene="clpX"
                     /locus_tag="MIW95_000886"
     CDS             23999..25249
                     /gene="clpX"
                     /locus_tag="MIW95_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBY3150233.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(25320..25847)
                     /locus_tag="MIW95_000887"
     CDS             complement(25320..25847)
                     /locus_tag="MIW95_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBY3150234.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(25835..26347)
                     /locus_tag="MIW95_000888"
     CDS             complement(25835..26347)
                     /locus_tag="MIW95_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBY3150235.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            26527..28890
                     /gene="lon"
                     /locus_tag="MIW95_000889"
     CDS             26527..28890
                     /gene="lon"
                     /locus_tag="MIW95_000889"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBY3150236.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFNSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     ARKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTAVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            28880..29491
                     /locus_tag="MIW95_000890"
     CDS             28880..29491
                     /locus_tag="MIW95_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBY3150237.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(29756..31126)
                     /locus_tag="MIW95_000891"
     CDS             complement(29756..31126)
                     /locus_tag="MIW95_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3150238.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYALGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDISGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(31301..31416)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            31704..32783
                     /locus_tag="MIW95_000892"
     CDS             31704..32783
                     /locus_tag="MIW95_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY3150239.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKATEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            33175..33855
                     /locus_tag="MIW95_000893"
     CDS             33175..33855
                     /locus_tag="MIW95_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBY3150240.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            33848..35227
                     /locus_tag="MIW95_000894"
     CDS             33848..35227
                     /locus_tag="MIW95_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBY3150241.1"
                     /translation="MINIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGVLLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            35398..36483
                     /locus_tag="MIW95_000895"
     CDS             35398..36483
                     /locus_tag="MIW95_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBY3150242.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            36537..36896
                     /locus_tag="MIW95_000896"
     CDS             36537..36896
                     /locus_tag="MIW95_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBY3150243.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
BASE COUNT        15072 a         3960 c         6453 g        11581 t
ORIGIN      
        1 agtatttgta aaaagtatga tattcaaatt gaagataaca taaatatatc taaattaaac
       61 tttattgaaa ataatgatat atgttcaata tttgcaaatt cactagataa tgcaattgaa
      121 gcatgtatgg atataaataa tgagctagaa aaaagaatag aagttaaagc tacatatata
      181 aataaatttg caataataaa atttataaat actaaggtaa atgatattaa acttattgat
      241 aaacgtattc aaactagtaa ggatgatgat aagatacatg gaataggact tgctagtatt
      301 aaatatatag taaataaata tggtggtgaa acaatagtta attactcaga taatgagttt
      361 atattaaaaa taatgatacc aataaaaagt taggagagta caaaatgaaa aaaatagcgt
      421 taaacttact taaaaacatc tcagcattaa gttttgggat agctgtatta tcagcaaaca
      481 gtgcaagttc ttgggtagca catcaggcaa aagagccaca agctttacaa aaactaaaaa
      541 aataacatta ataaaaggac ctcttcttat taaaggagag gttttacatg ttgaggagga
      601 aatatgttta aaagattatc atataaattt gccaatatat tagttaataa tgagattgta
      661 gaaagtgaag attttgaaat atatagatat ggatttgaaa ctttgattta tttcatagtt
      721 aatatttcgg tagcattatt tataggaatt atttttgata gatttataca tacagttata
      781 tttttaagtt gttattgcac tttaagacag ttcacaggag gttatcatgc taggaattat
      841 aaagagtgta cattaacatt tgcagttatt tatttgataa ctattttttc agctaataat
      901 atagatataa ataaatataa gtatttatta gtattattaa tgattattag tatactaaca
      961 atttacaagt tagctccttt agaacataga aacaaacctc ttagtgaaag tgagaaaaag
     1021 cattatagaa agactgtgca aaaaatacta ttcgtaataa tatgtttaat tattttatgt
     1081 aaaatactta atatttttca gcaatatgtg atttatgcaa taatttctat atattggatt
     1141 gctatattaa tatatattgg aatgaaggtt aataatgatc agtaagatta taaaacattt
     1201 tctaaattaa ttaatttcac taattaatat taattttaaa aaataatgtc aaggtaaata
     1261 tttgaactta ttaaatatag ttgaatctat tgtaagttgt atcaaaggat tggctaggag
     1321 gggcataaat atgagtagta ttaaggttat atcagacttt ataaccaaaa taattagaaa
     1381 aaatatttta tttgcaataa cagttattat tataaattac catctcatac aacgatggac
     1441 acatatatcc aatagaggaa tttcatttaa tgattactta tccttgagtg tatatacttg
     1501 tatgacaatt agtattttaa ccatgttaaa tgtaattttt tatatgagaa aaagttatga
     1561 tactgtaaaa atattatgta gaaataattt tgattatttt ttaggtattt ctgttgctgt
     1621 aactagaaac aactttatat tttgttgcat accaattata tatgctatag catactcaag
     1681 tttaataaga acattatcaa ttgtaacgat taaatcaact atatttatat tggtatttga
     1741 atggttttta ccacttacta ttttatctat agtgatatca tttatttcag ttattataaa
     1801 taatttaata ctaaagatta ccttatcatc agtattttta tatttaacat caactaagat
     1861 gttggataga gttattgata ttaaagaacc attttttaaa gcattattta aatcacttaa
     1921 tatatttgaa gatcaatcat atgcttacta ttctgatttt gtagggaaag ttaataattc
     1981 agcatatgtt atagataaga ttatacctac actatttgca attactttaa taatagtagc
     2041 atatctgata ttaaatgata ttagaaagaa acagattaaa tcattaatag ttatagtagt
     2101 gtacagtatt ttatttattg gattaaatta tatatcaata gatatgtcaa catatagaga
     2161 tgaggaacgt agttatggag agtatttaaa gaaatctaat attagtgata ttaaaataaa
     2221 atcgcatgat atgaagattg actttaaaag caagtcaaat ataaaagata gaattgtttt
     2281 attaaacaca tcaaatgaag agttaacaaa tattaatatg attttagatg aaatatttga
     2341 tataaaatat ataaaagtaa attcggaaaa tataaaattt aacaaaaaaa aggatttaat
     2401 tgaagttaat ttaaaggaaa gtttagagcc taatgacgag gtgacattag atatagagta
     2461 tgaaggctat attaatgtgt tgacatcttg gaatcaaaat aaatatatag ctactaaaga
     2521 tgatattatg cttccaccag gttctttagc gtggtaccca aaagtaaatc aatcaagttg
     2581 tatggcttat aaaataaatt taagttctaa aaataatgtc tattcaaatt tacctttact
     2641 gaataagact agtaattttt taaaaaaaga ttatatattt gaaggaaagt caaatgatat
     2701 tgcaatatat gcaggtgagt ttaaagaaga ggtaaaagat gatataaaaa taatctatcc
     2761 atcagatagt aatgtaaata tggatataga ggaaaaaatt aaatttttaa ttgaaacaag
     2821 aaatgaaata ttaggtgata gcaaagcatc tgtagatgct aaaaaatatg tagatgaagt
     2881 agataatgtt atggaatcaa taaaaagaaa aaaaattaat atgatattag ttgctgactt
     2941 acctagcaat gaatttttat atgaagactc aaagggagag aaaattagca taactattta
     3001 tcattggctt tcaggaaata tccttataat agataaataa taaaggaggt taatatgata
     3061 aacaagttag aaataagaaa tctaaatttt aattacaaaa ataaaaaagc tctggataat
     3121 ataaatttaa ccctaacaga tggagtagtt gcattattag gtcctaatgg tgctggaaaa
     3181 tcaacattaa tgaggttatt agtgactctt tatgaaactt caattggaga gatagaatta
     3241 aataatataa agtatagcaa aaacaatgag aaaatcaagg caaatgtagg atatatacct
     3301 caagattttg atatgtataa taatataaat gggcaagaat acttagagtt tgtagctaaa
     3361 atgagaggtg tatctaagaa tgatttaaag aaacatatac aaaaagtagt atcaaaagtt
     3421 aatctggaca agtttataaa taataaaata ggtacatact caggtggtgt aaagagacga
     3481 ctaggtatag cacaggcttt aataggtgat tctaagttaa tagttatgga tgaacctaca
     3541 gttggtcttg atccagagca aaggaatgaa tttagaagat tgttaccaga aataagtaaa
     3601 aattctatag tattaatttc aactcacata gtagaagaca tacaatttaa ctgtgacaaa
     3661 ttaattatat taaatcaagg caaaatatta tatgatggta caattaataa atttattgat
     3721 atggttgagg tgtattcagt agtagtttct aatgatgaat ttgaatattt agaacgtaat
     3781 attgatatta ttgattatag aagagttaaa gatggtgtgc aagtaaagta tataggtaaa
     3841 aatacagaga aacttaagga tagcttaagg atagatacaa ctttacaaga tgcttatata
     3901 aactttttac ataaggagtt aaaataaatg tgtatattgg agttaataaa atttgagctt
     3961 aaaaaaatag agcttaaaca tgtgttaata tttgtaatta tttctatatt atgcatattt
     4021 attacaggca tattagatta taatgagtta atattttata atgatgtacc tataatggat
     4081 acatattcta tagcaggatg gttaggaatt ataaagatat cagatgtttt tataccaata
     4141 atgtcagcaa tcacaataat ttatgtgttt tcaaaagatt atttgggtaa tataaatgag
     4201 atacttactt tatataataa aaaaaaatat aatcaattta tagtatttag atggtgtgtt
     4261 atattagcta tatatactgt attattatta atttcaatta caatagtata tacatttacg
     4321 tcagtgaaag gttttaacat agacaaaata gattttgttt atagtgtaaa gccaatagat
     4381 gtgtttttaa agacatttcc tacattatta tggtatacaa catttccact aatgatatta
     4441 acattaacaa aaaatagatt tacaacaata gcaatattaa ttacatatac atttgctgat
     4501 atatttttta ttttacatat gtacccattt gttagtatga ttaatgtaaa tggattttat
     4561 attcaaaaga tgttttatat gcctaataat atagatgtta gatttaatga attaaagaat
     4621 tatttcttta taaatagagg ttctctagta gttatttcta taataatagt tttttatata
     4681 gcaagaaaat caacggtttt aaaaaagagt aaatagttta atatattttt tataatgtta
     4741 tatttatata gaatagtgac tttaaaaaat ttaaagccac tattttttaa tatgttttat
     4801 ctatataact attatttttc taggtttttt aatatttcgt aatagtgtgt tgtggctatt
     4861 tcacttgact cgcctttttt acaaaaagat aatttagata gttttggaac tacacgttca
     4921 acgacaattt tattacctat cattttagat tcaagtatct tcaatgcttc taaaaatctc
     4981 ttatcctttt ttgcacgatt ataaaatgat aaaacgtaga cataaaggaa taggttataa
     5041 ttacggaaag gatattcaac ttgcataaat aatgtgccaa ttccatagtg acaagggccg
     5101 agtggttttt taatagtcca atggtcaagt agaaaatcta cagctttgtc aagttttgat
     5161 tctttattta gataattact aaaacggaat gcatttaata tattaagagt tggtagtgga
     5221 tttgagtatt cagtttctgg acctcgacca aaactaaatt tattgcaacg ccacccacca
     5281 tcagtatact gtgtatctaa aaaatgttga aacgtttttt gcattctaat gtcagatgca
     5341 tatcccatat gacagagtac gttagcagca tttgcagtat gacaagggta aatacctcca
     5401 tttggatata gtttgaaacg tccatcttct cgccatgtgc taaatatcaa gtcagcacat
     5461 tctttaagta aagtatcatc agggttcaca cctaactcta atagataaag tacactatca
     5521 agagtagaga aaggagaacc ttttattaaa cgcttatcag gtgtagtcca atagtctgca
     5581 ccattatcat acctgtgtga taatattatt tcaatatctg ataaagattg attttctaca
     5641 ttcatttttt accccatttc atattatatt ctattaaatc tatcaaaaac tttattataa
     5701 tattatttat atttataatt gatttatttt atttaaaatt gcatttatag ctgtatcagg
     5761 aacaactgct aaatcttctg cattgaagta ataaactata ttagaatcaa ataatatatt
     5821 tgaatttgga ggaaattcat catctccaaa ccaaagtata aatatcatag atgtattacc
     5881 catatatgta aacctataag ctaaatctcc catgtttact ttttcagctc caattttctc
     5941 cataactaac ttatatttgt ctatttgaga tgtaaaaact tctgaaagcc tatatagagt
     6001 cctgtttaaa aaatttggat aataaacatt gcctcctggt atttctttaa aagttacata
     6061 tttattagtt ctagggatac ctttagcatt gattaaatat ctaactatca agattttaag
     6121 ctcaaagtta ttgcaaaggc taccattact attccaaact tctccacttg gatagtctat
     6181 aatatattgt ctatcaaaaa aattgagtgt gaatctattt aaatcattat tgaaaggtat
     6241 attacttaaa ttactaattg tatgagggtc taattctttg agtttttttc gaacatagtc
     6301 aaatggaaca ttataattat tttttgttat attttcatcc ataatttttt cctccaattt
     6361 aaacaattaa aagtgtattt ataaaaagtt ttcttattat taattttatt atatattcaa
     6421 aatattacaa taataaattg ttaaataaat ttttatattt aagtaaatgc gtgtaaaatg
     6481 aatataaagt ttttgaaata taaagttttt gtatctaaaa tattttattt gtaaaatagt
     6541 aattaattat tgtaagctat aatatgagag tattatggtt aatgtgaatt ttaaagatat
     6601 tattaaagat ataaattaga atactattaa acggataaat ggaggagtta ttatgtctaa
     6661 gatattagta gtagatgatg agttagatat gttaaagttg attgaaaatg tgttgaaaag
     6721 agatggacat gaagtaaagg taattagtga tgtagaaaag gctatgaaca tggattttaa
     6781 ttattttaat ctaataatat tagatgttat gatgcctaag atggatggtt ttgaagtatg
     6841 taaaactata agaaataagg ttgattgtcc aatactgttt ttgacagcaa aaaatatgga
     6901 aagtgatatt atgtacggtc ttggcatagg tgcagatgac tatataacca aaccttttgg
     6961 tgtaggagaa ttaagggcta ggataactgc tcatttaagg cgagaaagta gagaaaaaaa
     7021 gaatttatta actatctcaa atgttcaatt taacttttta ggcaaagaag tttcaattga
     7081 tggtaaaaaa attaatttta ctaaaagtga atatctaata tgtgaatttc tagcaaaaaa
     7141 taagggacaa gtattttcaa aagaaagaat atatgagtcg gtatatggtt ttgatggaga
     7201 aagtgatata agtactatca cagagcatat aaaaaatata cgaagcaaat taaaacaata
     7261 tgatatttat acaatagaaa ctgtatgggg gatagggtat aaatgggtgt aataagtata
     7321 agtgttgctt agtgaaaata ttatatatat ctacaaaatg tttgtaaaat attaatctat
     7381 atattaaaat ttaatttata gattaatatt ttttgtaaat gacaaaattg taaggtattt
     7441 tgttataaaa tattaagatt catagtgtat actaataata tagtatttag tataaatctt
     7501 gagcaaagga tggttatttc taatggatag actaaagaat attagaaaga gtttactaca
     7561 gtatacattt ttatttttgt taataggtat aacaatttac ctagtgttta aaacattgga
     7621 tattaaaatg ctatcaaatg ttatggttat ggtagataag aagtttttat tcataggtgc
     7681 tttggcaata atgtgtcaca taatgcttga aggagtagta atgaagataa ttatagaaag
     7741 cactcacaaa gttaatatta gatttattgg ttttaagcta gccattatgg gattttatta
     7801 taatttaata actccttttg cttcaggaag ccaaccagta cagatatatg tacttaaaaa
     7861 atgtaaaatg ccacttagta aggcaagtgc agttgttacc aataagtcta ttattttcca
     7921 aatagtggtt actttttatt gtacaatact tgtattaatg aattttgcta tgttagaaaa
     7981 acaaatgaaa atgattatgc ctctagtgtt tctagggata gccattaatt catttgtctt
     8041 aattatgata atattagtta ttttaaaccc taaaaaaata aaatatttta tgagaattac
     8101 tataaagcat ttatcaaaaa ttaagttttt aaaattttta gataataaga ttgaatccat
     8161 agaaagtttt atagatgatt atagtaaagc aataaatttt tttgcaaaga ataaaaaggt
     8221 attaatttca actataatag taacttttat tcaattaagt gcttacttta gtgtttcatt
     8281 ttggatatat aaagcattta atttacaagg ttatagctat atttatatac tgacattaca
     8341 agcattttta tatatggcaa tatctccaat accaacacct gggaatgtag gagctaatga
     8401 attagccttt ttcactatat tcaaatcagt attccctcaa cctttgatgg gatatgcagt
     8461 atttttatat ggtggattta tgtactattt aattctgatt ggaagtggaa tatttactgt
     8521 aattactcac tatagaatga aaaatagaat tggcagaaat attgttttaa gtgaaaacta
     8581 aataagttta aagaatatat attgagtata ttaaaaaata tgtcataaga actagaatat
     8641 tttaattaaa tggttgaaat ataaagatta ttatgacata ttttttttga aatttaagag
     8701 gaattgtgag aaaattgttg aatttaatag atagtattgc aaaatattta tgattttgca
     8761 tattataaaa acactcatta tctttaagat aaattttact tatcagggga taaatctaag
     8821 gggggaattg tatggagaaa aaaattttaa cagtttgtcc atactgtgga gctggatgcc
     8881 aattatatct agtagtaaaa gataataaga tattaagagc tgaaccggct aatggtagaa
     8941 caaatcaaga aagtttgtgt ttaaaaggtc gttatggatg ggattttcta aatgacccta
     9001 aaattctaac acctaggcta aaaaagccaa tgataagaaa acaaggtgta ctagaagagg
     9061 ttgaatggga tgaggctata aactatacag cttcaagact aaatgaaatt aaagaaaaat
     9121 atggacctga ttctattatg ggaacaggtt ctgcaagagg acctggtaat gaagcaaact
     9181 atataatgca aaaatttatg agagctacaa taggaactaa taatgttgac cattgtgctc
     9241 gtgtatgaca tgcaccatct gtagccggtc tggcttactc attaggtagt ggtgcaatgt
     9301 ctaattctat accagagata gaaaatgcag atgttttatt tatatttgga tataatggag
     9361 cagattcaca tccaatagtg gcaaatagaa tagtaaaagc taaaaaaaat ggtgctaaat
     9421 taattgttac tgaccctaga gtaactgaat ctgcaagaat tgctgatata catctaccta
     9481 taaaaggtgg aacaaacatg gttttagtaa atgcttttgg aaatgtatta atagaagaag
     9541 gtctttacaa taaagaattt gttcaaaatc atacacaagg atttgatgag tataaagaaa
     9601 ttgttaaacc atatacagct aaatatgctg aaaagattac aggaatccca gaagagctta
     9661 taagaaaagc tatgagagaa tatgcaaaag ggaaaaaagc tatgatactt tatggaatgg
     9721 gtgtctgtca atttggtcaa gctgttgatg tagttaaggg acttgcatca attgctctac
     9781 ttacaggtaa ttttggtaga gaaagcgttg gaataggacc tgtacgtgga caaaataatg
     9841 ttcaaggagc ttgtgatatg ggagctcttc ctaatgtata tcctggctat caaaatgtaa
     9901 ctgatgataa gataagagaa aagtttgaaa aaacttgggg ggtaaaatta tctcctaata
     9961 atggttatag tttaactcaa gttccagact tagtactaaa agagaaaaag cttaaagctt
    10021 attatatatt tggagaagac ccagtgcaaa gtgacccaga tgcatcagaa gttagagaag
    10081 cattagatga attggaattt gtcattgttc aagatatatt tatgaataag acagcacttc
    10141 acgctgatgt tatacttcca gctacttctt ggggagaaca tgagggagta tatacatgtg
    10201 cagatagagg attccaattg atgagaaaag ctatagagcc acaaggagat gtaaaaccag
    10261 actggcaaat tataagcgaa atttctacag caatgggata tcctatgaat tataaaaata
    10321 ctaaggaaat atgggacgaa cttagacaat tatgtccaag tttcttaggt gctacttatg
    10381 aaaagataga aactcaagga tgcgttcaat ggccttgtaa gagtgaaagt atggaagata
    10441 aaggaactat gtatctgtat gaaggtcaaa agttttctac tcctaatggt aaaggtaatt
    10501 tgttcgctgc tgaatggaga ccccctatgg aagttgaaga tgatgaatac ccatttagtt
    10561 tatgtactgt aagagaagtt ggacactatt cagtaagaac aatgactggt aattgtagaa
    10621 cacttagttc attagaagat gaaccaggac gtgttcaaat aaattctaat gatgctgaaa
    10681 aattaggaat tgaagatgat gaattagtta gaattagttc aagaagagga agtgtaataa
    10741 caagagcaac agttactgat agagttaagg aaggtgcaac atatatgact tatcagtggt
    10801 gggttggagc ttgcaatgaa cttacaatag caaatttaga ccctatatct aaaacaccag
    10861 agtataaata ttgtgctgtt aaacttgaaa aacttgaaga ccaagaatta gcagaaaaat
    10921 gtgtaagaga agagtatcaa agtttgaaag acaaaatgac agctacaaat atctaatgat
    10981 ttattagatt taagatttat aaaaggcaga aaggctaata ttttatgaac aatatatact
    11041 caaaaactaa attacaagaa aatagtaagg ataaagtttt tgaaaatagt tatagtctag
    11101 tggactacaa atatttaaat tcttttaatg tagaagtaag caaaataaat gaacatgaag
    11161 tatcaataga taatgaagaa ataattgcag aatatccact tagttttatt ttaaatgata
    11221 aatatgtaaa tacattttta tgtaccccat ataatttaga ggaacttata gtaggattct
    11281 tgaaaacaaa aggatacata gaaaacaaaa aaagcttatt aaaatttgag ataaatgaga
    11341 gtgaacgttt tgcaaaagta actattcaag agaaacaaaa aaatcaagat gagcaaatta
    11401 tatttttaaa ttcactagac tatataacat gtgcttctgt aaagagcaat ataaaaataa
    11461 attatgatac gatttacagt ataatggata gaaatttaaa ttcatcagaa ttatttaaaa
    11521 atactggtgg ggtacatagc gtatctgtat ttaattttga taaaaaaata gtcacttgtg
    11581 aagatgtagc aaggcataat gcaatggata aggctattgg attttgtgtt ttaaatgaca
    11641 taacattaaa agataaaatt gtggctgtaa gtggaagaat atcattggaa atgatattaa
    11701 aagcagctat gactcaaatt cctattgtag tatcaaaatc agcaccgact aatttatcaa
    11761 tagagctttc taaaaaatta aatataacgc ttattggatt tgtaagagga agacggatga
    11821 atatttatgc aaatccacag agagttatca aaaagtgaga ataagtatat gtgtactaaa
    11881 aaagaagaac aaaaatgttc aaagtgtgaa atctgtggag tattaattga cgatactgat
    11941 aaggtgtata ataacacata caatttgtgc aaaaaatgta aacgaaatat aagtactgag
    12001 aaaatagtga aatatataaa tatatatgat gaaagtaagt agacatatga tgaaagtaac
    12061 tagagggttg gaaatataca aattgaaagg tgggataata tgaaaaataa aataggctct
    12121 tttgtaatag cggacccaaa caaatgtaca ggttgtagag cttgtgaagt agcttgtttt
    12181 acaatgcata atcagcataa taatgtgggt tatacagttg gaacagttga tatacctgtt
    12241 atacctagac tttatttagt aaaaggtgac aatttctgta tgccaataca gtgtagacat
    12301 tgtgaagatg ctccttgttt aaactcatgt ccacaaaaag ctatagtcaa agaaaacaat
    12361 attatgtctg taaatgaaga aaagtgtata ggatgtaaaa cttgtctgct agcatgtcct
    12421 tttggagcta tagacttact tccacaatat gaagatggta aagaagtaga acaagtaatt
    12481 ttagatgaaa ataagaagat agcttataaa tgtgacttat gtaaagacaa tgaaaaaata
    12541 gcttgtataa gtgcatgtcc tcaggaagca ttaaaacttg ttactccaat agatgacaaa
    12601 aaagctaaga atagacaagc tgcacttagt ttattgttaa caaataaata aaggatacag
    12661 gggtaggtga cgtttatgat aataaatata gataaggatt tatgtactgg atgtagagaa
    12721 tgttcaaagg tctgtccagt aaatgctatt gaaggtgaag agggaaagcc acaagagata
    12781 aatttagata gatgtgttat gtgcggtcaa tgtgttcaaa cttgtaaatc ttatgcctca
    12841 gttatagatg aaggatttga gtttttacaa gagaaaaaac aagaaagaga gataccagag
    12901 tctataaatg aacctatatt tgcagcatac aatgtatgtg atatagataa ggtaaagaaa
    12961 gctttaagtg acccaaatgt attcactatg gtccaatgtg caccagcagt tagagttgct
    13021 ttaggagaag atttcggata ttatttagga tacttaggtg ctggaaaaat ggcagcagca
    13081 cttagagctt taaattttga tagagtctac gatactaatt ttgcagcaga tttaactata
    13141 atggaagaag gtagtgaatt aattaaaaga gtgacagaag gtggaactct tccaatgttt
    13201 acatcttgtt gtccagcttg ggtaaaattc atggaaaaaa attatcctaa attaactaat
    13261 catttatctt cttgtaaatc acctcagcag atgggtggtg caatatttaa aacttatggt
    13321 gcagagataa ataatgtaga tgcatcaaag atatttagtg tttctataat gccatgtaca
    13381 tgtaaaaaat atgaatgtga tagagaagaa atggattcta gtggatatag agatgtagat
    13441 gtagtattaa ctactagaga actagcttat ttaataaaag acatgggaat tgattttaaa
    13501 aatttaagag aagaaaaatt tgatagtcct ttaggtagtt atagtggagc tggaactatc
    13561 tttggtgtta ctggtggggt tatggaagct gctataagaa caggatatga gcttataact
    13621 ggagaaagta ttcctgatgt agaagtaaaa caagtaagag gagaaaatgg ttttagaaag
    13681 tcaactatta aaataggaga cttagactta agagttggag tagtaagtgg attaaaaaat
    13741 gtaataccag tgcttgaaga cttggaaaaa ggaaaattag atgtagattt tattgaagtt
    13801 atgacttgtc ctgttgggtg tgtaagtggt ggaggtcaac caaaaatttt actagaagaa
    13861 tataaggaat tggcttatga aaatagaaca aaagcaacat acgtgcatga tgaacattta
    13921 gcaattagaa aatctcatga gaatcctgat attaaaaaac tgtatgatga atatctggta
    13981 gaaccattag gagaaaaatc tcatcatcta ttacacacta catattgtat aggaaaggaa
    14041 gtggctaagt gatgaactat tttgttatag cagaccctga taaatgcata ggatgtagaa
    14101 cttgtatgat aggatgtgta gttgcacatt cagatgaaga tattttttac caaaatccag
    14161 atgaaataaa ttttaatcca aaactttctg tcataaagac aaaagaagta agtgctccaa
    14221 tacagtgtag acattgtgaa gatgctcctt gtgcaaaggc atgtccaaat ggtggtatag
    14281 ttagggttgg gaacacaata aaaataaatg aagaaaattg tataggatgt aagacttgta
    14341 tgttagcatg tcctataggt gcaatagaca tagttacatt aaaagatgta gatgaaggaa
    14401 agctttgctt tagagaaaga atgacagcaa ataagtgtga tttttgtata gaatcgccag
    14461 aagggcctgc atgtgtaaat gtttgtccaa ctaaggcatt tactatagtt aaggatgagg
    14521 atatagatag taaagttaaa aataagagaa aattagctat attgtaatat aaaaaagatg
    14581 gagttttatt tatgattaaa agctccatct tttttattat aatatttata tatctaaaaa
    14641 tcaattattg cattagttta tctaatcttt ctttattcaa ttttttcatt cctatagaag
    14701 ttataataag taaagtatag gcaattataa ctgataaaat tataatagac caagaaaatc
    14761 tgtatccaaa attatcaact aacaatccaa ataatgttga acctaatgca aatcccactg
    14821 caaaaattaa ttgtacgatt ccatatatgc taccaaactc cttttgacca aagaaagagc
    14881 cagttaaata agcaggacca atcatataag caaatacaga aagacctttc aaaatggcaa
    14941 atacaaatgc aaaagtagaa gatttatcta ccaatagtaa tgataaagtt gctataagaa
    15001 ccaatacaaa agatataata agtggctttg tttgaccgaa tttatcaaac aataatcctc
    15061 caaataaatt accaatgaga gcaaataaag caaatgtgga gcctactgtt cctattagaa
    15121 taggatttag ataatttcta agataagttg gatattgtac ggataatgat gaaacatata
    15181 taccaacaaa tataaatcca atagtcataa tccaaaaata tttaattttt ttagcttctt
    15241 ttaatgtata accccatact gaaggagaaa ttgtatcaga tgctttctct tttttacctt
    15301 tagattttac cttttcatta atatctttag gcatcctgag tatgaataat attacaggta
    15361 taccaactat taatgctata attccaaata ttagataaga atgactgtat ccttgagttg
    15421 ctaataagcg aactgcaagt ggttgtagaa atatactacc aatagaacca ccagcaaaag
    15481 caaagcccaa agcttttcct ttgctttctt catcaaacca agcacttatt aaaacaggta
    15541 ttcctatagc agatataatt gtagcgccaa tttgaacaat acatgataca gtgtaaaatt
    15601 gccatagttc agtacatata gaaaaagtag caaaagcaat actagaaatt atacaaccta
    15661 ataaatatag tatcttaata ttgactttag aaaataatac acctataaaa ggtgatgcaa
    15721 ttgctgaggc aatagtacca atagtgaata ttagagaaaa agaagctaaa ctaaagcctt
    15781 ctccttctac aacataactt ataaattgag gctgtatact tgctacaata ctaaatggaa
    15841 cagcttgtat taacatacaa ccaagtacta taacccaagc atttgaaaag tttttaatgc
    15901 taaatttatt gtttagtaat gtattcatgg tatctctcct ttaggttatg atttgtagat
    15961 attaatctaa ttattctaat tttatatatt agttttggat agcctaacta tttaaatgta
    16021 atttgggtat taatataaaa attaactata aaaattgatt attttaaatg tatggggtaa
    16081 attaaagtat ataaataaat gaaaggatgg ataaaatgtc atttgaaaaa ataaataata
    16141 ataacctaga taaaatacac aataggaagt gtattatgct tgtaaacttt aataaaaaag
    16201 aattatcatt aattaaaaac gtaagtaact ttgctggaat aagagaccat ataatcttaa
    16261 gtgaaaaaaa cggagagaat ataataagag acattttaga tggaaatata tcaagtgaat
    16321 gtgaaaatgg gattaagagt aagtctgtaa tatttaataa cactccaagc acacaagtaa
    16381 atgcattttt agaaggtcta aaaaaagtga gaataaatag accttttaca gctatggtaa
    16441 cagagacatc tattgattgg acattgaata atttaataga aaatttagtt caagaacgta
    16501 atgctatgtc tttaggaaaa acaattgaac ataagtaaat ttaagatatc ttatagtaat
    16561 tttaactaat gtacattgag tatcatatta tattttagaa tataaaattt taaatgtggg
    16621 tagatagagg agatttgaga tgaaagtttt atttacaata aattatggaa aagaaaaatt
    16681 tgataaaata agagaattag gctatgatgt catacattac tctgaagata gtattgaaaa
    16741 taatgaggat gtaaatacag cagatgtact agttacatac aatccatttg agagattaga
    16801 tatttctaag atgaaaaatt tacaatatat acagacaact agtgtaggta tagaccaact
    16861 tccaaaagaa gaggtattga agagaaatat taagatagct aataatagag gcggatatag
    16921 tgtacctatt ggtgaaagta ttgtattgta tattttagaa atatataaaa atagtgcaaa
    16981 attttttaaa caacaacaaa ataaagaatg gaagatggat ttttctgtat cagaattgta
    17041 tggacaaaga gttggatttt taggaacagg tactatatct tcagaagcag caaaacgatt
    17101 aaaagctttt ggtgtagaaa tatggggtgt caatactgat ggaagtagta aaaaatactt
    17161 tgataagtgt tttgctactg aagatatgaa tattgtattt gaagaatgtg atatagtagt
    17221 tgtagcaatg ccttctacaa aaagtacaga tggaatagta aacaaagata tgtttaaact
    17281 tatgaaagat ggttctgtat ttataaatgt aggtagagga aatacagtta atcagaaaga
    17341 tttagaagaa tgtgtagaga aatttagagg ggtagcacta gacgtatttg aaagtgaacc
    17401 tttaaacaag gacaataagt tgtgggagtg cgaaaatgtc atagtaactc ctcataattc
    17461 ttgggtgtca gataaaaaca aagagagaac atttaatatg gtttataata atttgaaaca
    17521 ctatataatg gagaataaac ctgtagataa tgttgtagac atatcaagag gctattaaaa
    17581 ataaaaaatt ttgataaaaa taaaaactct tattagtcgt attaaagata ctaataagag
    17641 ttttttttgt aataaattta aagtcaaaaa agtgatatac taaaaaatat ataaatagga
    17701 gataattaat tatagggaaa tttcaaaata atttaatttt aaaaatatga agaggtgcaa
    17761 aaagtgagcg taaaagatga aattaataaa cttatagaga caataaacta ccataatgaa
    17821 aggtattata atcaagatag cccagaaata tcagattatg aatacgataa gttaatgaag
    17881 gagttaattt ctttagaaga agaacatcca gaactaaaga gaattgattc tccaacaaat
    17941 agggtaggag gtaagccatt agataaattc aatcaaattg tacataaaac tcctatgttg
    18001 agtttatcaa atgctttttc agagcaagat ttgagagatt ttgataagag agttcaagaa
    18061 tatgtgggag aaaatgttga atatgttgtt gaatttaaaa tagatggtct ttctgtaggt
    18121 ctaacttata acaatggaga gtttgaaaaa ggagcaacta gaggagatgg agttgtagga
    18181 gaagatattt ctcaaaactt aatgacagta aaaagcatac ctcttaagat aaatgataaa
    18241 aaagaactaa tagttagagg agaagtatat atatcaaaag ataattttag aaaagtaaat
    18301 gaacaacaag aagaacaaga acagccatta tttgctaatc caaggaatct tgcagctggt
    18361 tcattgagac aattagaccc aaaactaaca gcaaagagac cattagatat atttgtattt
    18421 aatatggaaa atatagaaga atatgattta gaaagtcaca gtgaatcact agagtactta
    18481 gaaaaactag gattttcagt aagtcagagt tataaagttt gtaaaagtat agatgaagtt
    18541 attgagcata ttgaatactg gacagataac aggggtaatt tagactacga aatagatggt
    18601 atggtaataa aagtaaacaa tataaaacaa agagacgaaa tgggatatac agccaaaagt
    18661 cctagatggg caattgcata taaattccca gcagaaaaga agaaaactaa aatagtagat
    18721 ataatagtag aagttgggag aactggtaca ataacacctt cagcaattct tgaaccagtc
    18781 agacttgcag gtactactgt aagtagagct acacttcaca atgaagatta tataagagaa
    18841 aaagatataa aaataaatga tacagtatta gttcaaaaag caggggacat aatacctcaa
    18901 gttttagaag ttataaaaga ggaacgtact ggagatgaaa tagattttaa aatgccagaa
    18961 aagtgtccag tttgttcaga gccaacaata agattagaag gagaggctgc tgtaaaatgt
    19021 atcaatatgt cttgcccagc ccaaataaga aggggaatta tacactttgt gtcaagggat
    19081 gcaatgaata tagatggatt gggagaatct ataattacac tgctattaaa tgaaaaaatt
    19141 attcaagatg tatctgattt atattatata aaaaaggaag atgtagtcga cctagagaga
    19201 atgggcgaaa aatctgctga caatttaata aattcaattg aaaaatcaaa agaaaatgat
    19261 ttatggagac ttataaatgg attaggtatt aaatttatag gagttaaagc agctaagata
    19321 cttgcatata actttaagga tttagatgaa gtaatttctg caactagtga gcaactagaa
    19381 gaattagagg aatttggaag tataatggca aacagcgttg tcgagttttt taaggaagac
    19441 aaaaatatga acgttataaa caagttaaaa aatgtaggag taaatacaaa atcattggat
    19501 aatacaggtg aaaaagtaga aaaaatattt gaagggatga aaatcgtatt gactggtaca
    19561 cttcctacat taaaaagaaa tgatgcgaaa gatatgatag aaaaaagagg tggaaaagct
    19621 acatctagtg ttagtaaatc gacaacattt gttttggcag gtgaagaagc tggttctaaa
    19681 cttactaaag caaatgattt aggaattaaa gttatagatg aagagagatt tttagaatta
    19741 ttaaagttat catcgaaaga tgaagtaaaa gctgtattag agagttaaaa agtcgcttaa
    19801 tttagtagaa aactactata attaatgata gaatagttca gtaagctaat aagattacga
    19861 aaaatgacaa taattaatat ttgacaaaat taaaaattat aaaaataaat ttataaaaaa
    19921 taaaaaatat gagaaaggat ataaaaaatg accagaagtt atgatagaag taacgaacag
    19981 ataagacctg taaagataac tagagattat acaaagtatg cagaaggttc tgttcttata
    20041 gaaatgggag agacaaaggt tatatgcact gcatcagtgg aagaaaaagt tcctcctttt
    20101 ttaagaaata gtggtactgg atggataaat gctgaatatt ctatgctacc aagagcaaca
    20161 caacaaagaa agataagaga tgcatctagg ggaaaaattg atggaagaac acaagaaata
    20221 cagaggttaa taggaagagc aataaggtct gttgtagact tgaataaatt aggagaaaga
    20281 actatttggg tagattgtga tgtgattcag gcagatggtg gaactagaac tgcatctata
    20341 acaggggcat ttgtagcagt tgcagaagca atatataaat tatataaagc aaagacaata
    20401 aaagagatgc caataacaaa ttttgtatct gcaattagtg ttggtattgt aaatggaata
    20461 cacatgctag atttatgtta tgaagaagat tctaaagcac aggtagatat gaatataata
    20521 atgactgata agtgtgagtt tgtagaagta caggggacag gagaagagaa acctttttca
    20581 agagcagatt taaatttatt gttagagtta ggcgaaaaag ggaataaaga attaataagg
    20641 cttcaaagag aagctttagg tgaaatagca gatgagatat tgggtatgga ttatggtaat
    20701 gaagtagtga ttgctactaa taatgctcat aagctagaag aaataggtga aatcttaaaa
    20761 gattttgaat ataaagtgta ctctttaaaa gatgttgatt tagcaggtat agagatagta
    20821 gaagatggaa aaacatttga gcataatgcg ttgataaaag ctagagcaat agcaaaaaag
    20881 actaaactta tagcaatatc agatgattct ggcttagaag tagatgcttt aggcaaaaaa
    20941 cctggtgttt attcagctag atatgcagga gaacatgcaa cagatgaaga aaatagaaaa
    21001 aaactgttaa aagctatgca aaatgtacca atgagcaaaa gaaatgcaag attcgtctct
    21061 gcaatagcag tagtttttcc tgatggaaaa gaatttgtag taagaggaat ttgtgaagga
    21121 atgataggat ttgaagaaaa aggtaaaaat ggttttggat atgatagctt atttatagta
    21181 aaaggatatg ataagacttt tggtgaaata cctagtgtta taaaaaattc aatcagtcat
    21241 agagcaaacg cactaaaact tatgaaacaa gagtttataa aaagggtagt gaagtaatga
    21301 aaataggcat agtaagcgac acacatatga tgattaaaaa tatggaaaaa acaataccat
    21361 atttgaaaga atgtgaccta ataattcatg caggagataa ctttactgat tcaagatata
    21421 ttcatagcat gactaatgta ggtattattg cagttaaagg taattgtgac tttgatgctg
    21481 ttgaggaaga agttgtcttt gaagtggcaa ataaaaccat attcttatgc catggagata
    21541 agtatggtgt aaaatatgga actaatatgt tagaaaaaaa ggcaactgaa gttgatgctg
    21601 atatagttat atttggccat acccataccc catttagaga aattaaagat ggtgtacttt
    21661 acataaatcc tggaagcact tcacttccaa gaggagtatc gtataaaagt tttgtcatta
    21721 tggatataga agaagatgac ataaaaattg aagaaatacg catataaagg tgtatttatc
    21781 taaaaaaatg gcaaaaaacg ctcaaaaaat taaattttat caaataagta caacgatatt
    21841 aatggaacac ttgtaaataa aaaaacttat gtgatataat taagtggttg aattataaat
    21901 agaatgaatt ataataccgt tatcgggtat tacataaata tcactaggag gattagaagg
    21961 atgaaagcag aactaattaa aaaagagggt aataaagtta ctttaaaaat aacagtagat
    22021 aacaataaat ttgaagcagc agtaaataag gcttataata aaagcagaaa taaatacaat
    22081 atacctggat ttagaaaagg taaagctcca agaattgtta tagagactca atatggaaaa
    22141 ggtatattct acaatgatgc tatagaaata ttattcccag aagtttatcc agaagctatc
    22201 aaggaattag acatagaccc aatagacaat ccagatttag atatagaaga aataagtaaa
    22261 gataatgggt tggttatggt tcttaatgta gaagttaaac cagagtttga attaggaaac
    22321 tacaagggta tagaaatagc taaggttgag aatactgtaa gtgacgaaaa tgttgatgct
    22381 aaactacaag aaatggttga aaaaaatgcc agattagtaa gtgtagaaga caaagcttta
    22441 gaagatggag atacagctat aatagacttc gaaggttttg aaaatggtgt agcttttgat
    22501 ggtggtaaag gtgaaaatta taacttagtt ataggttcaa acacatttat accaggattt
    22561 gaagaacaat tagtaggtaa aaaagctggt gaagaagtag aagttaatgt tactttccca
    22621 gaagaatatc actcacaaga tttagctgga aaaccagttg tatttaatgt aaaaataaat
    22681 gatgtaaaag taaaagaatt atctgcactt gatgatgaat ttgctaaaga tactagtgaa
    22741 tttgattctt tagatgaatt aaaagcagat gtaagagcta agttagaaga agaagctaaa
    22801 aatagagcag atgcagaaac tagaaattca gtagtagaaa aagttgctga aaatacagaa
    22861 atagaaatac cagaagttat gatacaacat caaatagata atatgttaaa tgaacttaac
    22921 taccaattac aatatcaagg atttggatta caacaattac tagagatgac tggtagaact
    22981 atggaagaat taagagaaga gagaaaagaa gatgctaaaa aattagttaa gtcttcttta
    23041 gtattagaag caataactaa agctgaagga attgaagcaa cagaagaaga atttaaggct
    23101 gagttagaaa aaatggcttc tgcttacaat atggaagtag aaaaaataga agcttcttta
    23161 agagatgctg acaaagaaga tataaaaggt caaataaaaa taagaaaaac aatagattta
    23221 ttagttgata atgcaacaat agcttaataa aataaacgct aactaatata gttgacgtta
    23281 tgaaatgttt agtatataat gatgtgcaaa tataatttag ttaaatgcgt ataataattt
    23341 acagtagctt aaaaaagcta ctgtaaatta tatagaataa agggggtatg aaaccatggc
    23401 attagtacct gtagttgttg aacaaacagg aagaggagaa agatcttatg atattttttc
    23461 tagactatta aaagacagaa taatcttctt aggagatcaa gttaacgatg ccacagcagg
    23521 gcttatagta gcacagctat tatttttaga agctgaagac ccagacaaag atatacattt
    23581 atatataaat tctccaggag gaagtataac ttctggaatg gctatatatg atactatgca
    23641 atacatcaaa ccagatgtaa gtactatatg tataggtatg gctgcttcta tgggagcatt
    23701 cttgttggca gcaggagcaa aaggaaaaag actagcacta ccaaatagtg aaataatgat
    23761 acatcaacca ttaggtggag ctcaaggtca agcaacagat atagaaattc atgcaaagag
    23821 aattttaaaa ataaaagaaa ctttaaatga gatattatct gaaagaactg gtcagccatt
    23881 agaaaaaatt aaaatggata ctgaacgtga taacttcatg agtgcactag aagccaaaga
    23941 atatggttta atagatgaag tatttacaaa gagaccgtag atagagagag gtgttaatat
    24001 gtctaaatat gaagaaaaac gacaattaaa gtgttcattc tgcggtaaaa atcaagatca
    24061 agtaagaaga ttaattgcag gtccaaatgt atatatttgt gacgagtgtg ttgaactttg
    24121 tgacgaaatt attcaggaag aaatagaaga tactatagat gaagatacta catctttacc
    24181 aaaaccaaag gaaatgatgg agattctaaa tgattatgta ataggtcaag aaaaagctaa
    24241 aaaagcttta tcagttgcgg tttataatca ttataagaga atatacagta aaaaatctag
    24301 tagtaaggat atagaaattc aaaagagtaa tatactttta ttaggaccta caggttctgg
    24361 aaagacatta cttgctcaaa ctctagcaag aactctaaat gtaccttttg caatggcaga
    24421 tgctacatca ttgactgaag ctggatatgt tggagaagac gttgaaaata tacttttaaa
    24481 acttattcaa gctgctgatt ttgatataga aaaagctgaa agaggtataa tatatataga
    24541 tgagatagat aagattgcaa gaaaatctga aaacccatct ataactagag atgttagtgg
    24601 tgaaggtgtt caacaagcac ttctaaaaat attagaaggt actgttgcaa atgttccacc
    24661 acaaggagga agaaaacatc cccatcaaga atttttaaag atagatacta ctaatgtatt
    24721 atttatatta ggtggagctt ttgatggatt agagaagata attcaaaaac gtggtggaga
    24781 taaaacacta ggttttggag ctaaaataga aagtaaaaaa gaattagatt tagggaagct
    24841 gtatgaaaaa gtacttccag aggatttatt aaaatatggt ataataccag agtttatagg
    24901 aagaatacca gttttagcta ctttagagtt gttggatgaa gatgcattaa tgcagatatt
    24961 acaggaacct aaaaatgctc tagtaaaaca gtataaaaag ttattggagt tagatgatgt
    25021 tgagctagaa tttgaagaag gtgctcttcg tgcaatcgct aaaaaggcta tagaaagaaa
    25081 tacaggagct agaggactta gaagtatagt tgaaagcgta atgatggaaa ctatgtttga
    25141 agtaccatct agagataata taaagaaggt tatagtaaca gagaaatctg tgaacgaaga
    25201 ctcagtaaat cctatcatag tacttaagga tcaagaggaa agtgcataag atgatgaaaa
    25261 ggagctttaa aagctccttt ttttatgtta atatgctaaa tataaaatag gtatcattat
    25321 cataaaaatg tgagtaatat acctagaata ccaaagacga atacaattgc aatggaacca
    25381 atttttcctt tatataaaag ataaaatgat gctataaatg caattattcc atataaatcc
    25441 caattaactt ctttggcaga tgaaaaagct gctgataata tgaatcctag agtaattggt
    25501 ctaagaactg tcaataagtt ttctaccttt ttattatttt taaatttaga aaataacttt
    25561 gatataatag ttagacctat taatgcaaaa attgtaacac caatagttgc agctagagaa
    25621 ccaaagaagc cagcagtctt aaatcctaca aatgtagcac tatttattgc tacaggacct
    25681 ggtgtagatt gtgatatagc aagtaaatcc aaaaactctt gattagttat aaagtgatat
    25741 ttagatataa attcttgctg gataaatggt agcatggcat aaccgccacc aaaactaaat
    25801 gcccctattt taaggaatat taagaagagt agtattattt ctgacataga ctatatacac
    25861 ctcctccaat tattaaataa atagggctta tattgaatac accaacagat aaaactgcta
    25921 taattaatag tagtatgtta gttttagact ttggtagttt cttgaacatg ctcgtaaaag
    25981 aatacaatat aagagctatt acaacaggcg caactccttt aaaaaatcca tctaaaattt
    26041 tagatgatga atttttaaag tatagatacg ataagactaa gactatacaa aatgatggta
    26101 aagcagagcc taaggtacat ataagaactc ctggtatacc ataaagttta tatcctaata
    26161 atacagatat attaactgcc agtactccag gataactttg agctatggat aaataatcaa
    26221 taaattcttc ctgctctaaa agtttttgtt tgtttacaag ttcatcttgt ataagaggaa
    26281 ccatagcgta tccaccacca aatgtaaacg caccaatttt taaaaaggtt aaaaacaatt
    26341 tcaacataat agaaacctcc aaaagataaa taacatctaa gtatactaat attaatatta
    26401 tatcataata aaaagataat atagtatgat agtattctaa gaatttatca ttaatgttga
    26461 aaattaactt gttcaaatat ataatattaa aagcaaagca attagactag aaaaggagta
    26521 atgttgatgg aacaaaatta tactaaaata gatcatgaat taccattaat tcctctaaga
    26581 ggattagcta tatttccata tatgatatta aattttgata taggtagaga gatatctctt
    26641 aaagctttag accaagctat gatggatgaa gaacttatat ttttaacttc tcagaaagaa
    26701 gcagaggtag atgagccagg agaagaggat ttttatcatg tgggaacaat ctgtaaagtt
    26761 aagcaaatga taaaattacc aggagataca gttcgtgttt tggttgaagg tgtatcaagg
    26821 ggtagagtaa agaaaataga acaagaagat gggtatttca gagcagtaat agaagaaata
    26881 gtatttaata gtgataattt ggatagtgaa acagaagttg aaattgaagc atttgtaaga
    26941 aatgtatttg atgcctttga agagtatata aatattggaa atagggtatc accagaaata
    27001 ttaatatctc tagctgatat agaagatgta gatagattta tagatacgat agctgcaaac
    27061 atatatctaa aatcaagcca aaaacaagaa atattagaag aatttgatat tagaaaaaga
    27121 cttgaactta tttattctat attattagag gaaatagata tattaaaaat agaaaagaaa
    27181 ataactttaa gagtaaaaaa acagatgaat aaggttcaaa aagaatatta tcttagagaa
    27241 caattaaaag ctatacagaa agaattaggt gaagaagaag atataaattc tgaagcagat
    27301 gagtatagag aaaagcttaa aaaaataaaa gcacctaaga caaccaaaga aaaaatagaa
    27361 aaagagatag ataaattttc aaaaatatct tctatgtctc cagatgtatc tgtaagtaga
    27421 aactatttag atactatatt ctcattgcca tggaataaag agacaaaaga taaattagat
    27481 ataactaagg caaaagatat attagatgaa gaccattatg gattagaaaa agttaaggag
    27541 agaatactag agtatctagc tataagaact cttgctaagt cacttaaagg gcctataata
    27601 tgtctagttg gtcctccagg aacaggaaag acatctatag taaaatcaat cgccagagca
    27661 ttaaatagaa aatttgtaag aatatctttg ggtggagtta gagatgaagc agaaataaga
    27721 ggtcatagaa gaacttatgt aggttcaatt ccaggaagaa taattaatgg ggtaaaagaa
    27781 gcccaaacaa aaaatcctgt attcttattt gatgagatag ataagatggc agcagattat
    27841 aaaggtgacc cagcttcagc aatgttagaa gtattagacc cagagcaaaa taaggacttt
    27901 gtggaccatt acttagaaat accttttgat ttatcaaaaa tactatttgt tacaacagca
    27961 aatagtctag gtaatatacc aagaccatta ttagatagaa tggaagtaat agaagtatct
    28021 gggtatatag aagaagaaaa attaaatata gcaagaaaat acctacttcc aaaacagatt
    28081 aaagaacatg ctttaaaaga aaactttata aaaatagatg atgaaacttt aagaagtata
    28141 attaaccatt atacaagaga agctggtgta agaacactag aaagaactat tggtaagatt
    28201 tgtagaaagg ttgccaaaaa atacgttgaa gacccaactc ttgaagaggt tgttataaat
    28261 aagtctgacc tagagacata tcttggaaaa gacatgttta agtatcaact tgcagaagtt
    28321 aatcctcaaa taggattagt aaatggtctt gcatggacag cagttggtgg agttacactt
    28381 gaagtagaag taaacgtact taaaggtaaa ggagaaattg tattaactgg aaaattagga
    28441 gatgttatga aggaatctgc gaagacaggt atctcttata taaggtcaat agtggacaag
    28501 tttgatatag accctgaatt ttataagacg aatgatattc atatacacat tccagaaggc
    28561 gctgttccaa aagatggacc atcagctggt ataactatgg ctcttgcagt aatctcagca
    28621 cttactaaaa gacctgttcc tggaaatata gcaatgacag gagagataac tttaagaggt
    28681 agagtcttag cagtaggagg ggttaaagaa aagttattag cagctcatag agctggtata
    28741 acaaaagtat taattcctaa agaatgtgaa gctgatttag atgaaatacc agaaaatgta
    28801 aaagaaaaaa tggagtttgt gcttgtagaa catatggatg aagtattaga acaagcttta
    28861 ttaaagagtg gtgaaaataa tgaaaattag aagttcagag ataactatga gtgctgtgaa
    28921 taaatcacag tatccagcag aaggaatccc agagatagca ttggctggta ggtcaaatgt
    28981 tggaaaatca tcaataataa atacattgct taacagaaga aacttcgcta gaaccagtca
    29041 aacaccagga aaaactagaa ctataaattt ttatctaata aataatgaat tttattttgt
    29101 agaccttcca ggctatggat atgcaaagat agctaagtct gaaaaagaaa agtggggagg
    29161 tataatggaa agatatctag aaagtagaca agagttatgt tctatattcc tacttgtaga
    29221 tattagacat gagcctacag cagatgataa gcttatgtat gaatggataa agcactttgg
    29281 atataactgt gtagttattg caactaaggc agataaaata tctagaggac aatatcaaaa
    29341 acacataagt ataataagaa aaaaattgca aatggaaagt agtgaaaaag taattcctgt
    29401 ttcttcacta aaaaagactg gtgttgaaga attatgggaa gagattgtaa atcaatacaa
    29461 tcaacatgga tatgaaataa cagtagatta aatttaaaat gttaaatatt gacataagat
    29521 ttatttgaat aataaatgta ataatagtta gttttaatta tattaatatt aaagcaaaat
    29581 ataggatgcc ttaaaatagt tttaatgaac tttttttggc atccttattg tttataaaca
    29641 ataaatatca gtaattttgc taaatactaa taaattttag taaggtatgg gaaaattagt
    29701 ataaattagt tcaactaaaa aattattgac ttatgtagtc agcaataaat agcatttaag
    29761 aaatagattt atctgttttt ctcataaaga aaacagttag tataagccct ataaaacata
    29821 ctgatgctgc aatccaataa gactgattgc tagctataac ataggctgat gtgtgacttt
    29881 taaacttttg attgtatgta agactttgtg caaaaaaact actaaaaaat gctgttccaa
    29941 gagcaaatga taaattacga atagttgcaa gaaaactgcc tccgtacccc tgtttttgag
    30001 gaggaacatc tgctaaaata ctactattgt ttggtgaact aaatgctcct agcccaagtc
    30061 caaaaataac taaacataca attaaaagat acaatggaga atctgctttt aaaagtgcca
    30121 ttccaactag tgagatggtc ataaatgaaa aagatgctgt tagaatgttt tttgcaccgt
    30181 gtttatcaga aagtgaacct gcaataggtg ctataagtac cataataagt ggatgtacag
    30241 tcattacata accagccatc ataggagata aattttttaa ttgttctaaa taaaatggaa
    30301 gtagaaaaga tactgccatt tgaggaaaat aacaagctac tccaatgata ttgcctacag
    30361 taaagtttct atttttaaat agaggtaggt caataaagct ttgttcaaat tttacttctc
    30421 ttaatgccaa caataagaag attataagtg ctgttatacc taaatacaat cttgtattac
    30481 ccttctcatt cattgcaagt aatataagcg tagtgcaaga tgcgaaaagt agaagacctg
    30541 aaatatcaaa gctcttgttt tgttcatcca aaacaggttt aggcagaaat ttaatgccaa
    30601 gaaagactgc tataatacca ataggtatat tgataataaa aatagaaggc catccaaaat
    30661 gttctaaaat tatacctcca attacaggac cactcatatt tcctatacca actacagctc
    30721 cagtgattcc tatagctttt cctttttcat ttgctggaaa tgcattggaa actataccaa
    30781 gcccagtagc cattaaaata cttgctccaa ctgcctgaaa gattcttgaa gaaattaatg
    30841 ttgataaatt gtttgacata ctacacagaa aagaacctat tgtaaatata aaaaatccta
    30901 atgcgtatag acgatttgaa cctactttat caccaagttt tccgaaaaat aacatagtag
    30961 ctgttacaac aagcatataa gctgttacaa cccactgaat ttgtgacatg ttaattttaa
    31021 agtcagcagc aattgtagga tttgcgatat ttagtatact tccatcaaga gtagacataa
    31081 atacaccaat acagatgata atcaatacta tccatttttg tttcatgata acactccaat
    31141 cttaatagat tttataagta cgatataatt gtaaaataaa caacaaaaaa agcacctgta
    31201 tattaggtac cttgaatgtg tctaatttca tgaataaata cggtagttga aggatttgta
    31261 tagattactc atgtaaaatt acataaaggt actcatatac tattttcttc taccatccag
    31321 attttactgt cggctttgga atatgaccaa attatgccat aaggctcgcg ggcttttacc
    31381 gccgataggg aatttcaccc gtttatgaag aaaaacagat gtattttgta atattatttt
    31441 aacataaaat tataatggaa acaattggat ttaagtaata tccagctgta ttacaataca
    31501 aatatttcaa tatggacact taaattgtat ttatagatag aataaggtac agtatgagaa
    31561 tctataataa ttagataaaa ttaatatgtt attagtatta gatggtaagt tttaaatttg
    31621 tcaaaatcag aaagctgtaa taaaattctt ataatatgaa tatcttaatt aagagatatt
    31681 gtaaattatg aggaggcgct aatatggaag gaaaggtaaa gaaggcattc ccaggtggaa
    31741 atacatcaca aggctcatat tctttttttg actatataat tcctgaaaat gtaaaaagag
    31801 ttttttgttt aaagggtgga cctggagtag gaaaatcctc acttatgaaa aaaatttata
    31861 aagagttttt gcatagagga tatgatattg atttatatca ttgtccttct gacccaagtt
    31921 cgttggatgg tttagttata aagaaattag gcgtggtact tatggatgca actgccccac
    31981 atactatgga cccaaagcta cctggagctt tggatgagat aattaattta ggtgattact
    32041 ggaatacaga agaattagaa aagaataaac aagaaatatc agtttatgat aaagatatta
    32101 gtaattcatt taaaagagca tttaagtttt taaagtcagc agaaccaatt tttagagaca
    32161 tagaagaaaa atattctgat tgtatggatt atggcaaagt aaatttggtc acagaagaat
    32221 ttataaaaag gttatttgat ggagttaaaa gcacaggaaa tctaaaaaga gaaaagcatt
    32281 tatttggttc agctataact cctgtagggt gtttagatta tacagaaaac attttaaaag
    32341 atgtaaagaa agtctattat ttagatggag aaataggaac tgggaaaact acattattaa
    32401 acaaagtata taagaaagct actgaaaaag gtttaaatgt agaagtttat cattatccac
    32461 ttattccaga gaaaattgaa actgtactat taactgattt agatataggc ataactataa
    32521 gtacgacttt taaagaagaa gatacaattg atttaaatca gtttttaaat agagaaaaac
    32581 tacttaaata tgaagaagat attaaatttg atgaaaaagt tttagatgat ttaatttgtt
    32641 gggcagtact aaatcttaag agagccaagt ctaaacatga tattatagaa agttactata
    32701 gtcctaatat gagatttgat gaagtaggga aattaagaga taagttaatt aaaagaattt
    32761 taaaatatga ggaaaactta taaacgtata tgtttaaaat taatgttaat aataatataa
    32821 tttttagtaa attggtgtag ctttgaggct atgccatttt actttttttt gctaaaaaat
    32881 gatattaata atctccaaag ccattatttg tttttcataa ttattatttc ctttcctaat
    32941 ttatgttttt ttataatatc acaacaatgt ttattgaaag tttatataat tttaagaaca
    33001 tctaaaaatg catacttgat attggtaaat tagtgatagg agttcaatgt atgatattat
    33061 atactaaaga tgaattttaa tatataactt tgatataata agcatatgta aatgtttaat
    33121 atttaattaa agaaaattat catataaaaa taaatttgta aggagcaata cagtatgaaa
    33181 aaacaatatg tgtttataat actagcaagt actattttag gtatacttgt tggaaccata
    33241 tttagagatt atagcaatga agaaacagtt tatacaagca aagacaagtc aataagaaaa
    33301 gaaataaaaa tgacaagaaa atctataaaa gaattaacca aagagaaaga caagttagat
    33361 aaagaaatta attcaatgaa acaaaatcaa aaaaattctg aagaaatatc agaaatagaa
    33421 acacttagag atagtctatc atattcagat gtaaagggta gtgggataat attaagtata
    33481 gatgctatag atgaaaaaac agggaatata gctaatagta tagactataa taagattctt
    33541 gtaaaagtta taaatgaatt aaaagtaaac ggaggaaaat acatagaaat aaatggtcaa
    33601 agaattaatc aatattcaga agttgtactt gcaggaagcc atataaatat aaatggagtt
    33661 cctgtagcac aaccatatga aataaagtgc attggagata aggagaaatt atctaattat
    33721 gtagataaag gtagtaaata tttagatagc atacaaacca attatccaat gaaaataagc
    33781 ataaaaactg aagaaaatat ctctatgaaa aaaatgaata tacctaataa attaaagtat
    33841 ataaagggtg attaatatta tattagagga aagcagtttg ccaatgttaa taccagaaaa
    33901 acttgcaaag aaatataatg tatttccagt tgagtttcaa gatgataatt tagttgtaga
    33961 aatagaagat gaggatattt atgctctaca ggacttgaga ttagctacag gtaaagaaat
    34021 tatattgaag aaggagaaaa aagaacttat aagtgaaaag attgaaaaat attataatag
    34081 ctttgaactt gatgaggatt atgcaaaaaa attatttgaa aatcttttag aaaaagctat
    34141 aaaagaaaat gcaagtgata tacatataga accatttaaa gaacatttaa tagtgagaat
    34201 gagggtagat ggagaactaa aggaagtatc aaagttttta gttgatgtct atccatcatt
    34261 gtctacggtt ataaaattaa aggcatcaat ggatataaca gaaaaaaggc ttcctcagga
    34321 tggtagggtg gatataaagt taggtggaaa tttaattgac attagagttt catcaatacc
    34381 aactgtctat ggtgaaaaaa tagttttgag gatacttaat agagatactt ttttaaaaga
    34441 caaattagaa cttggttttt cagaggaagc tataaaatct ataaatcgta taataaataa
    34501 aagagcagga gtattattag tgacaggtcc tacaggtagt ggaaagacta caacagtata
    34561 ttccttatta aatgatttaa aaggaataaa taaaaatatt atgactatag aaaatcctgt
    34621 agagtataag atggatggca taagtcagat tcaagtaaat tcaaaagtag ggttgagctt
    34681 tgatgtaggt cttagagcga tattaagaca agacccagat ataataatgg tcggagaaat
    34741 aagagatgca gaaacagcta agatagcagt aagagcagct ataacaggtc atttagttat
    34801 aagtacttta catactaatg atgcagtatc ttccatagct agattactag aaatgcaaat
    34861 accaccatat cttttaaatg cctctcttat aggtgttata tctcaaaagt tggttagaaa
    34921 agtttgtaat cattgtagcc atgagattat gataaaagat aattttagtg gagatgtaaa
    34981 tactaaggta gctgtagggt gtgaaaaatg taatgataaa ggatactttg gaagaacagc
    35041 aatttatgaa atcttagaaa ttaatgatga tataaagaca tgtataagaa atatggagga
    35101 ttctagtact attaaagatg tggcacaaaa taatggaatg ataacatttg aagatagctg
    35161 taaaagacta ataaatgaaa aaataacgac attagaagag tgtttagtag taaatgaaat
    35221 gatttaactg aattgtatag ttaaacttcg tttggtcagt tgtctttatt taatctattt
    35281 taattaagat gtttgagtat aaattatatt taagtgaaat aaaattaaat gcattaaatt
    35341 agaaaatatt aagtttattt aattgtaatt aatataattt ggaaggtatt atagtacatg
    35401 agattatcta aaaatatatt ttataattta gaaaagtttt atttagtatt aaatagaatc
    35461 agtgataaag aaattagagt aatttgcaaa gagataggca tattgctgga atctggatgt
    35521 gaaattacaa aaatatttga aatcattgag agtcagtcaa gtaaaaaagt aaagaatttg
    35581 cttagtatag tatcaaatca tattcaaaaa ggtaactcaa tagcagaatc atttcaaata
    35641 acaggtatat tttctaaatt ctttataagt atgataaaag caggagaaac tagtggaaac
    35701 ttagatataa taatgagtga tttatctaat tattatgata aagaatacaa attaaaaatg
    35761 aaaataataa ctatttcaat atatccaata atacttataa tattatctat cttatcaatg
    35821 ttgtttatat tcgtatttgt aatacctaat tttcaggtgg tttttaccaa caatggaatt
    35881 gaaccacctt taataactag agttttaatg ggaatatcta cagtggttac aaataactta
    35941 gcttatataa tatttagttt tatattgttt tcaataggaa gcatttattt ctttataaca
    36001 aatgataata taaaaaaatt gattaattcg ttaaaattca aaataccttt tataaaaaaa
    36061 attaatcagt tagtagctac aacaagattt tcaagggcat tgtccgtttt aattagcagt
    36121 ggaatacaaa ttgtggaagc tatagacatg tcagctagtg ttatagacaa cgaatttatt
    36181 tatgaaaggt taaagatttc taataattat ataaaaaaag gaaatagcat aggtaaatct
    36241 ttgaatttag caaatgtttt tccaaaatta tttatttcaa tggcaaatac aggagaagaa
    36301 agtgggaaac tagataaaag tctaaatgta ataaataaat tttatgaaaa tgaattaaat
    36361 aacaaaatag agcaaataat gaaatttata gaacctatgc taattgtatt tatgggatta
    36421 attattggca cttttatttt agcaatggtt gttcctatgt ttgatgcaat tactaatttt
    36481 tagagagaaa aataaatttt attagatgaa acttttgaaa aggagagaat atttaattga
    36541 taaatttgat aaataaaaaa cgaaaaggat ttacacttgt tgaaatgatt gtagtagtaa
    36601 ctattttagg cgttatatct agtatagcat tagttaagta tagtaaggtt caagaaagtg
    36661 ccaaattaaa tgcagactat acgaatgctg ctaatatagt aactgcagct agcatggcaa
    36721 ttaatgatga tgaaaagaca atagactctc taagtgtaga aacattgaag gaaaagggat
    36781 acctaaatac tgttccagtt cctcagagta catcaggtaa attcgaactt gtcataaatg
    36841 atagcggaac agatataagc gtaaatataa attctaaaca attttatcca aaataatatt
    36901 ttatattgat tgctgtaagt tttaaaatta tttttattta agaaggagat ggctataatt
    36961 aaaaaaattc taaacttaga aataaatgat gattatataa ggcttgtatt tttaagaaaa
    37021 agtcataaaa aagtttttat agataaatca attataaaag agatta
//
LOCUS       DAGQWR010000020        36456 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.020, whole genome shotgun sequence.
ACCESSION   DAGQWR010000020 DAGQWR010000000
VERSION     DAGQWR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.020"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..2487)
                     /locus_tag="MIW95_000897"
     CDS             complement(<1..2487)
                     /locus_tag="MIW95_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBY3150244.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQST
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANQG
                     VYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGG"
     gene            2701..3399
                     /gene="yunB"
                     /locus_tag="MIW95_000898"
     CDS             2701..3399
                     /gene="yunB"
                     /locus_tag="MIW95_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBY3150245.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(3611..4618)
                     /locus_tag="MIW95_000899"
     CDS             complement(3611..4618)
                     /locus_tag="MIW95_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBY3150246.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNSEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISNLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            4846..5730
                     /locus_tag="MIW95_000900"
     CDS             4846..5730
                     /locus_tag="MIW95_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY3150247.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDKKNKIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            5932..7482
                     /locus_tag="MIW95_000901"
     CDS             5932..7482
                     /locus_tag="MIW95_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBY3150248.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKEKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            7511..8146
                     /locus_tag="MIW95_000902"
     CDS             7511..8146
                     /locus_tag="MIW95_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBY3150249.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSEDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVFLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPNLDPVINILPEEFFFHSGMMILILILLASILLFVMYRLYKSFKIK"
     gene            complement(8257..8517)
                     /locus_tag="MIW95_000903"
     CDS             complement(8257..8517)
                     /locus_tag="MIW95_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBY3150250.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMGKFQDLVKITSKK"
     gene            8734..10017
                     /gene="hflX"
                     /locus_tag="MIW95_000904"
     CDS             8734..10017
                     /gene="hflX"
                     /locus_tag="MIW95_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBY3150251.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTSESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGENGITLDVNFDEEDFNIYREYILEK"
     gene            10025..10702
                     /locus_tag="MIW95_000905"
     CDS             10025..10702
                     /locus_tag="MIW95_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBY3150252.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEAGEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            10788..11231
                     /locus_tag="MIW95_000906"
     CDS             10788..11231
                     /locus_tag="MIW95_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBY3150253.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTNCVLAPEFQE
                     GGFYKVKDALDMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            11537..12181
                     /locus_tag="MIW95_000907"
     CDS             11537..12181
                     /locus_tag="MIW95_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBY3150254.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVVVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITNGTGKIILGEEFYLSEQNGEILI"
     gene            complement(12267..12644)
                     /locus_tag="MIW95_000908"
     CDS             complement(12267..12644)
                     /locus_tag="MIW95_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBY3150255.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(12644..13030)
                     /locus_tag="MIW95_000909"
     CDS             complement(12644..13030)
                     /locus_tag="MIW95_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBY3150256.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            13209..13964
                     /gene="nadE"
                     /locus_tag="MIW95_000910"
     CDS             13209..13964
                     /gene="nadE"
                     /locus_tag="MIW95_000910"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBY3150257.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYRAEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            14047..14766
                     /locus_tag="MIW95_000911"
     CDS             14047..14766
                     /locus_tag="MIW95_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBY3150258.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(14934..15938)
                     /locus_tag="MIW95_000912"
     CDS             complement(14934..15938)
                     /locus_tag="MIW95_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBY3150259.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            16204..17100
                     /locus_tag="MIW95_000913"
     CDS             16204..17100
                     /locus_tag="MIW95_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBY3150260.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            17238..18500
                     /locus_tag="MIW95_000914"
     CDS             17238..18500
                     /locus_tag="MIW95_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150261.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            18576..19769
                     /locus_tag="MIW95_000915"
     CDS             18576..19769
                     /locus_tag="MIW95_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBY3150262.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LKRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            20398..21192
                     /locus_tag="MIW95_000916"
     CDS             20398..21192
                     /locus_tag="MIW95_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBY3150263.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            21286..22626
                     /locus_tag="MIW95_000917"
     CDS             21286..22626
                     /locus_tag="MIW95_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBY3150264.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            22763..23995
                     /locus_tag="MIW95_000918"
     CDS             22763..23995
                     /locus_tag="MIW95_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBY3150265.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            24035..25177
                     /locus_tag="MIW95_000919"
     CDS             24035..25177
                     /locus_tag="MIW95_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBY3150266.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGTDIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGIGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASSTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            25188..25982
                     /locus_tag="MIW95_000920"
     CDS             25188..25982
                     /locus_tag="MIW95_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBY3150267.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFAPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            25993..27015
                     /locus_tag="MIW95_000921"
     CDS             25993..27015
                     /locus_tag="MIW95_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBY3150268.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELEVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEVLPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            27114..28490
                     /locus_tag="MIW95_000922"
     CDS             27114..28490
                     /locus_tag="MIW95_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBY3150269.1"
                     /translation="MKEQWYKDIFARILSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKDKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(28822..29166)
                     /locus_tag="MIW95_000923"
     CDS             complement(28822..29166)
                     /locus_tag="MIW95_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150270.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            29654..30664
                     /locus_tag="MIW95_000924"
     CDS             29654..30664
                     /locus_tag="MIW95_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBY3150271.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEESTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            30800..31288
                     /locus_tag="MIW95_000925"
     CDS             30800..31288
                     /locus_tag="MIW95_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY3150272.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            31464..33392
                     /locus_tag="MIW95_000926"
     CDS             31464..33392
                     /locus_tag="MIW95_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY3150273.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFILSNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            33567..33977
                     /locus_tag="MIW95_000927"
     CDS             33567..33977
                     /locus_tag="MIW95_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBY3150274.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            33999..34301
                     /locus_tag="MIW95_000928"
     CDS             33999..34301
                     /locus_tag="MIW95_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBY3150275.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(34517..35209)
                     /locus_tag="MIW95_000929"
     CDS             complement(34517..35209)
                     /locus_tag="MIW95_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBY3150276.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(35235..36422)
                     /locus_tag="MIW95_000930"
     CDS             complement(35235..36422)
                     /locus_tag="MIW95_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3150277.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRIPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSISYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
BASE COUNT        13647 a         4357 c         6553 g        11899 t
ORIGIN      
        1 accgccacca ttatcacctg tgttattatt gttattatta ttttcttctg gttggttatt
       61 gtctggtttt tttgtatctt cagatgatgg ttgagaactt actttagtag gagcagtacc
      121 ttcaataaat gctgctttag agccagaaga ttgctttcct ccatcctcta aattaatttt
      181 tgtaaaatat acatttttag gaacttcaaa ttcagtaacc gttaaattag catgtatttt
      241 ttccataatt ttttcccata gttttgcagt actagtactt ccatgtggta cttctctacg
      301 ttttatagtg ttaccagaat catcttttct accagcatca tctccaatat aagttgcacc
      361 aacataataa ggagtgtaac cgacaaacca agcatcatat gcactatttg tagtacctgt
      421 tttacctgct acaggcattc cctttggaag tgtagcaaca ccaccagtac cttcagtaat
      481 tacgctttct aacatatctg taacaacata agctacttca gggtctacaa ctttatgttc
      541 ttcaggagca tttttaacta ataattgacc atcaaaggta gttatagtag taaaaataga
      601 aggttctaca taaacacctt gatttgccaa tgtaccataa gcagcagcca tttgaagtgg
      661 agtaatacca ttcgccatac cacctagtgt caatgatggg aattttctat cacttgcttc
      721 agatgaacct gagtgattat tctttaaagt tgttatacca aagttcttta agtaatccat
      781 cattatatct acacattcat cataagagtc tccaagcatt tcagcagttt tgacagctat
      841 cgtgtttgaa gatttagcaa gtgctttacg taaactcata gaaccatgac cagcagtagt
      901 agttcttgga ttccatttat tattttcttc aaacttatat cctcctcgag aatcacttag
      961 agcagttgat tgtgttattt ttagggtatc aatagcaggt gtatatattg ctaaaggttt
     1021 tatagaagaa cctggctgat gagggttagt agcacgattc aaagtttttc taccacttat
     1081 atttcttcct ccagctagtg ctcttatttc accattttta taatctaaga ttaccattgc
     1141 agcttgtggt tggctaactc catcagggcc gaccatatgt cctgggaaat tgctatttct
     1201 atcaaattct tcttctaaag catcttgcat tttagggtct atagttgtgt ttacaattag
     1261 accaccattg tagaataagt tttgagcttc atcatttgta tgtccttgtt ccattaaagc
     1321 atttataact tcactttcga taaggtcttc tacagatgaa gaaactttat ctgatgattg
     1381 aggcaatttt atttctattt tagctttgat tgcttcatca tattcacttt gttttatata
     1441 tttcaattct aacattttct ttaaaacaat ttcttgtctt tttttagctt cagggttact
     1501 tacagccttt ctaacaacta atgtacctgc ttttaattgt ctataagtat cattagatat
     1561 aagctcccaa gattttattt tttcaatcat atcaaagtcc acttgagtag ggtcatctaa
     1621 atcatcatca gttgtgttaa caaaaaataa taatttattt tctaaatcct ccttagtttc
     1681 atttccctct aacttagaag ttttataggc tgaaaatctt gatggatttt tagttgaacc
     1741 agcaagaagt gctgactctg ctagagtgag gtcagctgca gacttatcaa aataaccacg
     1801 tgcaccagcc tcagcacctg ctagacctct accaacgaag aagttattta aataagtttc
     1861 tagtatttta tctttactta ctgttttact catctcatgt gcatagtaaa tatcctttat
     1921 tttacgaggt atagtttgct gttcagttgt taacagcatt ttggatacct gcataggaat
     1981 agtactacca ccttgcttag tacctgtaag ggtctttaat actgacctaa ataaaccttt
     2041 aatatcgaca cccttatgct cataaaaacg ctcatcttct attgcaatta cagcatgttg
     2101 aagataaggg gaaatcttat ctaatggaac gggtgtcttt ttatttacac ttggagctgt
     2161 agacaatgtt tttccattag cgtatttaat agtagttgtt tggttcgtct gtttatctaa
     2221 aacagcttct gtaactggag ttacatctct tagtgacgca aatactagac cagcagtacc
     2281 tgctgtacca acaactagta aaactaaaaa tactatccca aatactctta gttttttaaa
     2341 tttatcgctt tttttagatt ttttcttgtt ttttgttgaa cgagtcttgt tagctgaagt
     2401 tctattaatt ggtttatttg tattaccaga tgagctaact ttttttctcc tgattttatt
     2461 tccattttta tcattatcgt tattcataag attttaacct ccttacttaa tgcaatatta
     2521 taacacaaat aggagtagtt ttgtaattaa tagaaaaaat gaatttgcta taatattctg
     2581 tacattgtat atgtataaac gtaatatatt aaaaagtcta tatttttatt ttatttattt
     2641 ttgacaaata ttataaaaat tatgccaaag ctacatataa ataaatagga ggggataatt
     2701 ttgaatagaa tagtagatag aaaaacaaaa aatgaattta gaaaaatcat agctattttt
     2761 ttagtaattc ttttcttgtc tgtatttata ggcagtttta tatatataga taaaacttta
     2821 agaccgacaa taacagtttt agcagagact aaagctcttg aactagcaaa taggtcgata
     2881 aataaagctg ttgctgagat ggtagaagga aaaataaatt atgaagattt aatggatata
     2941 caacttgata ataatggtaa aataactatg atacaagcaa acactataat gatgaatgaa
     3001 attgcatctg caatagcttt agaaatacaa gatgaattaa aaaaggataa gacagcaagt
     3061 tcttacatac ctataggaac agcattagga agtcctatat tagctaaata cggtcctaaa
     3121 cttgaagtgt ctattgaacc tataggaaca gtatcagtaa actttaaaac ggaatttgag
     3181 tcttcaggta taaatcaaac tagacataga atatatttag aagctcaaac acaagttaaa
     3241 gtagtaatac cacttataac ttcaacaaaa caaattaaag ctcaaatacc tatttgtgaa
     3301 actataatag taggtgatgt tccagaaagt tatgtaaata ttccagaaaa aaatctagga
     3361 aatgttttac ctaacactgg aaagaatacc aacaagtaat ataaagttgt atatttttaa
     3421 ataaattcaa aataatagtt tataaataaa atttttggaa ataatattaa tataatttta
     3481 ataaaaattt atattttata ctatgtaaaa tatctaatat atttggtctg taggttaaat
     3541 aaaagtacct ttagaccaaa tatattatta ataacttaat aacatcttaa aacttattta
     3601 tttatattat ttacaacatc catttaataa atacaccata acctacagct gggtcatttt
     3661 caattgcatg tgacactgct ccgattattt tattatcttg aactattgga gtaccactca
     3721 taccttgtac aataccacca gtttgagcta aaagcttttt atctgtaatt ttaattttaa
     3781 aacttttact ttctggatgt ttttggtttt ctatatctat aatttcaata ttatattttt
     3841 tacacttatt aaagttatct tgtaaaatta tttgagcttt tcctaattta acttcatcta
     3901 aagatgctac tttatatttt tttaaattgg aaatatcaaa gttattaatt tgacctctta
     3961 ttccaaaatc agtattttct gtaaagctac caataatatt ttctcgtttt gcatttatac
     4021 atccaacact acctttataa gatttttcta tattaaggtg cttagttgta gaaattgaac
     4081 ctgttttaat aggtattttt cttgcattac caacacttat tggatgacct acagctccaa
     4141 actctgatgt atctggattg atatacgtta aagtagcaaa tccagaaagt aaattgttaa
     4201 aactaatttt ttctaatatg tgtttggtag ttttcattgt tataagttgt ccacctctat
     4261 ttaataaaat agatacttgt ttttcttctg gtaaagcatt taaggtattg gcaaagtcac
     4321 tatatccatc aacttcagaa ttatttacct ttactatagc atcacccaat ctaaatggag
     4381 atgcttctcc aggatttcta actatgatat tttttaattc tgcgtcaatt tgaagtacgt
     4441 ttccaattgg tattaaatat tcaggttcat ttttttgtaa attttgtgca taaattaaat
     4501 tatttgagaa aaaatataat aaaaataaaa atgttaaaac aattgtaaat tttgacttag
     4561 ctaagttggt ttttttactt aaagtattat ttttaaaatg aaaattttta agttgcataa
     4621 aacacctctt tcttattaaa ttattttaat aatagaattg aagttttctt gctgcttcta
     4681 tattatgttg tgcaagaatt atgttttgat gcttggaaaa ctgactcaat aacaccttag
     4741 ttttcataaa aataaataaa ataaatagtg tatatgataa aataaataca tattcatata
     4801 aataaaaata aacaataact gtctagcata agaaaggagc aaaaaatgaa aaaatataga
     4861 ttgttagtta cttgtataat tgccatggga atcataatta taggaacatc aaatccagtt
     4921 agcaattttt acaagactac aaatttgatt agttcaggaa atatcacaaa tgaaaaagca
     4981 gataaagatg aaaattcaaa caaaaataat aaagataaaa atgaagacaa atctaattct
     5041 aaaagtaata aagataagtc atcagaggac aaatcgtcag ataaaaaaaa taaaattcaa
     5101 aaaaataaaa aacttttaat ttgtatagac ccagggcatc aaggaaaagg agatagcaat
     5161 ttagaaccag tagctccagg ttcttcctct aaaaaagcga gagtatcttc aggaacagag
     5221 ggtattgcta caaaaaaacc agaatatgtt ctaaacttag aagcatctct tgttttaaag
     5281 agtatattag aatctaaagg ttataatgta ataatgacta gagagacaca tgatgtaaat
     5341 ataagtaatt cagagagagc aatacttgct aatgataaaa aagcagatat ggttgtaaga
     5401 atacatgcag atagtttaaa taattcttca aaaactggag catctatatt gattcctgaa
     5461 aaagatggaa aatatacagc tcctatatat gaagagagta ataaatgtgc tgaatttatc
     5521 aaacagaata tggaacaatc aggtatacaa atcaatggaa tcgttcaaag aggtgatttg
     5581 actggattta actggtcaaa agtaccagcc gtattagtag aaatgggatt tatgagtaac
     5641 tataatgaag accaaatgat gtcaaatcca gactaccaaa gaaaaatgat gcagtgtata
     5701 gctgatggat tggatgcgta ttttaaataa gatatgaata gagaaaaagt taggttggta
     5761 ttattttcct aatttttatc tctatttttt attgtacata atgcaaagaa gaagtttatt
     5821 ttaggttaaa ttgtcgtaaa tataactaaa aatattggga atacttgatt taagtttaaa
     5881 aagaatatat tatagtatag aatgtgtaaa atttttttga gaggtgttga aatgctagta
     5941 gtagaaaatg tgagtcatgg atttggtgcg agaacaatat tagaaaatgt atcctttaga
     6001 cttagaaaag gtgaacatat tgcactagtt ggtgctaatg gagaaggaaa atcgagtttt
     6061 ttaaatataa taacaaagaa gcttatgcca gatgctggaa atataaaatg gtcttcaaga
     6121 gctacagtag gttacttaga ccaacatacg gtattaagca aggaaaaaac tataagagaa
     6181 gtgcttagag aggcttttaa gcatatgttt gacttagagc aagaaatgat tgctatgtat
     6241 gacaaaatgg gagaagctag tgatgatgaa atgagtaagc ttcttgaaga aacagcagag
     6301 atacaaacta tacttgaaaa tagtggtttt tatatgatag atgcaaagat acaagaagtt
     6361 gcaaatggtc ttggtcttgg agaaattggt cttgataaag atgtaactga cttaagtgga
     6421 gggcaaagaa caaaagtact tcttactaaa cttctacttg aaaatccaac tattcttata
     6481 ttggatgagc ctacaaacta tttagacgaa gagcatataa catggcttac taagtacctt
     6541 caagagtatg agaatagttt tgtcttagtt tctcatgata ttgaatttat aaataataca
     6601 tgtaatgtaa tatatcatat ggaaaatgga gagttaaata gatataaagg aaattatgat
     6661 gaatttgtaa gacttaatga tataaagaaa cgtcaagaag aacaagctta tgataaacaa
     6721 gttgaagaaa gaaaaagact agaggatttt gtagctagaa ataaagctag agtagcaact
     6781 agaggaatgg ctaacagtag acaaaagcaa cttgataaga tggaaatatt agaaagacca
     6841 aaagaaaaaa taaaacctac ttttgctttt aaagatgcta gagcagctag taaaataatt
     6901 tttgaaacag agaatttagt tttagggtat gatgaagcat tgacaaaacc acttaatttc
     6961 catttagaaa gaggaaaaaa gattgcctta aaaggaatga atggtattgg gaaatctact
     7021 ctattaaaaa ctttacttgg aataataaaa ccatttgaag gaaatgttaa attaggtgac
     7081 tatttggaag ttggatattt tgaacaagag agttcaaggg aaaatagtaa tacacctatg
     7141 gatgaagttt ggagtgaatt tccagggctt acaaactttg aagttagaca ggctcttgca
     7201 aaatgtggac ttacaaatga acatataacg agtcaaatga gagtattaag tggtggagaa
     7261 gctgctaagg ttagactttg caaagtaatg ttaaaaaata taaatttctt agtattagat
     7321 gagcctacaa atcatttaga tgttgaggca aaagatgaat tgaagaaggc tatcaaagag
     7381 tttaagggaa cagttctttt agtatgccat gagccagaat tttattcaga aattgttgat
     7441 gatgtatgga atatagaaga ttttaccact aaaatcgtgt agtgatttaa cttattgctg
     7501 gggggatttc atgaaaaagt ttctaaatgt acttttttca gtttgtatta gtattgttat
     7561 aatagtaggt gttattaatt ttactgttgg atttaaacaa ctttattatt ttgacattga
     7621 ttacttaaat atatcagagt tatctggttt atctgaagat gatataaaat tgaactacga
     7681 ttatctaatt gattataact taaataagaa tgtgtctgag ttcaagttac caacattaaa
     7741 atcttctcca caagggaaga tacattttga agaagtcagg aatatatttc aaaatataaa
     7801 taaattagct aaattattat tggtagtttt tttagtagga attatattga gtgtaaaaaa
     7861 taaaaatata aaaatcttga aaactacatc aataacactt ataattatgc cattattatt
     7921 aacagttcca attttattaa attttgaaaa atcatttatt atttttcata aattattatt
     7981 tagaaatgat tattggatat ttaatccaaa tttagaccca gttataaata tattgccaga
     8041 ggaatttttt tttcactcag gaatgatgat tttaatatta attttgttag caagtatctt
     8101 actatttgta atgtatagat tatataaatc tttcaaaata aaataagata gcaaaaacga
     8161 attatattaa aggttataca ataaaaatag ctatgtaata atatttaaac taagagtagt
     8221 cgataaatat ttatgcatag ctatttctaa tagaaattac tttttactag tgattttaac
     8281 taaatcttga aatttgccca taatgtaatt gtaattatct tttttgtgag gtaacatgca
     8341 actactgcaa tctttgatac ctttatctgt atacttaaag tttccaccac agttttctcc
     8401 taaagcatac aaaggacaat agcaaaatag acagttaaac tcttctggtt tattagtttt
     8461 atgacaaggg aaaaattcgc aatctttgtg attaaaaaac ttataatttt cgcccatatt
     8521 ttcctcctaa ttaaataaaa tttaaatttt ttatactata taagattttg gtgataatat
     8581 aattatacta aataatttta tgaatagtcg ataatatagt attattaatc atagtattaa
     8641 tggtataatt agtcaacaga aaaataatgt aaaaattatg taagtgtatt ttgtaattag
     8701 aatatgtttt ataacaaaaa atgaggtgat taagtgaaag aaattcaaga aaaagcactt
     8761 cttgtaggtc tgaatttgac tacaatagtg aaaaaaaatg atgatataga cactagtgaa
     8821 tctatggaag aattaaaaga gctaacaaaa gctgctgggg cagaagttgt aggtagctta
     8881 attcaaaata aacattctgt agatgcagct tattatatag gaaaaggtaa agtagaagaa
     8941 ataagagcct atagtgactc tttagatgct actttagtaa tttttaatga tgaattatct
     9001 ggtgctcaaa tcagaaatat agagaatgtt gttggaagaa aagtaataga tagaactact
     9061 ttaattttag atatatttgc tcaaagagca cttagtaaag aaggtaaatt acaagttgaa
     9121 ttagcacaat taaaatatag attgccacga ctttatggaa tgggtggtga gatgagtaga
     9181 acaggtgcag ggattggtac tagaggtcct ggagaacaaa aactagaaat agataagaga
     9241 catatactta ataaagcagc agacataagg cgtgaactta aagaagtaaa aaagaatagg
     9301 gagacacaga gagttaagcg tttaaagtca aatataccga ttgtagcact tgttgggtat
     9361 acaaatgcag gaaaatctac tcttttgaac gaattaataa agacacacaa ggattatgaa
     9421 caagaaaaag aagtttttgt aaaagatatg ctttttgcaa ctttggatgt aactctaaga
     9481 aaggctcttt tgccgaataa aaaagaattt ttagtagtag atactgttgg atttgtaagt
     9541 aaacttcccc atgatttagt agaggcgttt aaagcaacat tggaagaagt acagtatgca
     9601 gatttaattt tacatgtaat tgatgcgaca aatactagtt atgaattgca aaaaagtacg
     9661 actgaaggtg tattaaaaga acttggtgta aatgataaaa aacatatact tgtatataat
     9721 aaagtggata agctagaatt ggatatatat cctaaaagtc aagaagatat tgtgtatatt
     9781 tctgcaaaac aaggcataaa tatggataag ctattaaata tgatagaaat tgctttaatg
     9841 gaaaatactt attcagtaag cttgatgtta ccttatgaga gaggtgatat atttagtaga
     9901 ataaaagata aatataatgt ggagaatttt gagtatgggg aaaatggcat aacgctagat
     9961 gtaaacttcg atgaagaaga ttttaatatc tatagagagt acatactaga aaaataggtt
    10021 gaatatggat ataaattggg aaaatataga taatcttgaa gattatttta taacctatct
    10081 tctttataaa gaatctaaaa cagtatctca aattagtaaa attagaaata tttcaagtac
    10141 tgaagcaggt gagcaactta tacaagcaaa attaaaaata aaggaaatgc aaaaagatga
    10201 ttttgaggca tctaaggata ttttagataa atttttagaa ctagataaaa ttaagagact
    10261 tgattttatg gatagcttag atgacgaaaa aatggtatac tttaagagaa aggttttcaa
    10321 aagaatcctt gtggaaaaaa atgctgaaga tttaattgtt ttaatttggg cgacaggtga
    10381 gctaaaagat gatagattct taaagttgct tcatcagctc acaaaccaca gacactcaga
    10441 tattagaaga ataacttatt cagcaatcag aaagattgaa tcaccaagta gtagagaagt
    10501 actgcaaaaa ggtctctacg ataaaaatgc acaaactaga cagtattgtg caaaggctct
    10561 ttcaaaacta ggggatgaaa acagtttaaa gattttacag cagttgaaag acaagaataa
    10621 gaattttgaa aaagaatatg tgcttagagc ttatgacgaa gccattaagt cattggaaaa
    10681 tgcaaaaaat ttgaaaaagt aattttttat taaagcaatt tgtttactta atgatgtatt
    10741 tctagagttt aacaaggtaa taaataaatt aatgggaggg gtttcaaatg atagtaagac
    10801 gttgtgcagg tggagtagtg ttttatgcta ataaagtact tattgtaaaa aatgataggg
    10861 gagaatggac acttccaaaa ggaaagatac ttggaggagg acttccatat gagagtgctg
    10921 ttcaaagagt taaagtagaa actggcatag atgctaaaat gatagatgtg gctggcgata
    10981 ctatgtatga atttttctca agaagtagac aacaagaagt ttgtaatgca ataatgtggt
    11041 atgttatgga agcttgtaac actaattgtg tacttgctcc tgaatttcaa gaaggtggat
    11101 tttataaagt aaaggatgct ttagatatgt tatcacatca taaagagcaa gctttagtag
    11161 aagtatctta taaaaagttt aaggaattaa agaaattagt ttcagagggc gatgatgttc
    11221 cagcaaatta attgtgaatt tagaggattc tgaggaaggg atagcccctt ccttttttaa
    11281 ttgaagcagt tttaagcgta aattatatag tctctttaaa ttatgattta tcaatttaga
    11341 gctttagtag tttatattta aaatcagtaa gatatattta aaataaaaat taaaaagtat
    11401 acaattttat tatataattg tcttatgata ttgattaaag aagtattaaa agtccattta
    11461 tttttaaata gaataatttt atagatatat aatttgatgt gtatattaga aaatgattaa
    11521 aataggagga aatacaatga gcaattacag gacattacat gaatttggaa cagatgaaat
    11581 aactattgaa aagtctgttt ttattggata tgctaagcct ataaagagtg aagaagaagc
    11641 attagagttt ataaatgaaa taaagaaaaa gcataaagat gcaactcaca atgtgtgggc
    11701 ttatactgta ggtaaaaata tgaatataca aagatatagt gatgatggag aaccacaagg
    11761 aacagctgga atacctacat tagaggttat aaaaaaagaa gatttgagag atgtagtggt
    11821 tgttgttact aggtattttg gaggaacaaa attaggtgca ggcggtctag ttagagctta
    11881 tacaaaaggt gctaaattgg gacttgaagc aggtaagata atttataagg taatgtatca
    11941 agaagttaag gttaagatag actatactca acttggaaaa gtgcaaaatg agttaatgaa
    12001 tttggggtat tttataaaag atactgtata tgaggataat gttgagatag tagtgtattc
    12061 aagattagaa gatgtagaga aattatcaga aaaaatgatt gatataacaa atggaacagg
    12121 aaaaataatt ttaggagagg aattttattt gtctgaacaa aatggagaaa ttttaatata
    12181 atctaaaagg ttattagaat agttaatacc tcaaatagcc aattagtatt tttaagtgaa
    12241 aagccattaa attgatttaa tctaaatcaa tttaatggct ttttatcgga ttttgcacag
    12301 aatccttttt ttacttcaaa taaaccgact aaaagtaaaa atatcccaaa tatttttttt
    12361 aatatatctg aagacagata attagctaat agtgaaccaa atacagctcc taaaacccca
    12421 aacacagcta taggtataac caatttaaaa attacatttt tattttttat atgtatagca
    12481 agtgctataa gtgtcatagg gatagatgaa agtaaattta cgctttgtgc tattttagtg
    12541 tctatgccag caagtaaaac gagtgcaggt ataaggattg ttcctccacc cattcccatt
    12601 cctccaataa ttcctgcaaa aaatccaatt agagcaaata acattaaaac accatcctta
    12661 atgctgcaat tatcataatt attccaaaag aaattctaag aaactttcca gtaaccttat
    12721 ttagtaattt agctcctata ataccaccaa gaatacttcc aactgcaact ttaattgtta
    12781 agttaacatc ataagtacct ttagatacat ataaaacaga acttgctgtc gttaagaata
    12841 ttattataga aagagcagtt gcatgagatt tatgttcttc taccttaacg atgtcattta
    12901 atattggaac taaaagagta cctcctccag aaccaaaaac tccatttata aatcctgtaa
    12961 aaactccaat tatcgtattt tttaagttaa aatcaaagta ttttttcttt aaatcatcag
    13021 tttttttcat aaaaatcacc ttctgaaata gttttaccaa aataggcaaa ttatatttat
    13081 ataaaataag attaaatata atctttcatg tttgtttaat tttgcataaa aaaaaacata
    13141 ttaaaacaac atattaatat atggtataat atattaaaac aaagtagcaa atttgagagg
    13201 agataacaat gtcaaatata aaaatacaaa tagacaaaac tgtagaatgg cttataaata
    13261 aggttaatga ggcaaatgca aaaggattaa ttgttggtgt atctggagga attgattcgg
    13321 cagtagttgc taatttaatt aaaaaggcat ttccagaaaa ctctatggga gttataatga
    13381 gcataaaaag taatcctcaa gatagagaag atgcacttaa ggttatagag ggatgtgata
    13441 tagaatacct tgatttagac cttatagaac cacaaagtgc tatattagac atggtagtag
    13501 gaaatttaaa agataaacat ttatatagag cagaatattt aaagatgaca gatgcgaact
    13561 taagagcaag agtaagaatg agtacaattt atacaattgc taacaatcta ggatatttag
    13621 ttgttggtac agacaatgct gcagaaatac acacaggata ttttactaaa tttggtgatg
    13681 gtggagttga tattttacct attgcaaatt taactaaagg agaagtatat gaatgggcaa
    13741 aagagcttgg tgttcatgaa gatttaataa acaaagctcc ttcagcaggt ctttgggaag
    13801 gtcaaacaga tgaagatgaa atgggaacta cttacaatat gatagatgct gtgttagaag
    13861 gtagattgga tgaagttcct aaaagagacc aagagataat agaaagactt catagattaa
    13921 gtgaacacaa gagaaaaacg cctgcacaac ctcctaagtt ctaatttact atttataaga
    13981 tttgtgataa aatttacaag tgatgaaaat tagaataatt aattgtacgt attaaggagt
    14041 ggaaatatgg gacgtatagg taatataatt aatagaaaag gtaaacaaga tgctcaaaga
    14101 gcaaagatat ttactaaaca tgctagagca atagcagttg cagctaaaga aggtggagca
    14161 gacccagagt acaatgcagc gttaaaaaca gctatagaaa aagctaaggc agataatatg
    14221 ccaaatgata atatagatag agctatcgct aaaggtgctg gagcaggcgc tggagaagac
    14281 tatgaaacta tagtttatga aggatatgga cctggtggag ttgctgttat agtagaaact
    14341 ttaactgaca acaaaaacag aactgctggt aacgtaagat actattttga caaaaacgga
    14401 ggaaacttag gaactagtgg atgtgtgtct tttatgtttg ataaaaaagg acaaatatta
    14461 gttggtttag gagatggagt ttcagaagaa gaattgatgg atgttgcatt agaagcagga
    14521 gctgaagatt tcataacaga agaagatggt tatgaaataa taactacacc agaagatttt
    14581 tctagtgttc gtgatgagtt aaaagctaaa ggatatgagt ttatatctgc tgatgtgaag
    14641 atgatacctc aaactacgac tgtattgact gaagaatctc atttaaaaat gatgaataaa
    14701 ttagttgata tgcttgaaga agatgatgat gttcaagata tatatcataa ctgggaagta
    14761 gaataaatag tataagcata ttgaaattta taagcattga atcaatttta acatgtgata
    14821 aattttatag aataagaaat tactaaaata gacatacgca cctctaacct atatttgcat
    14881 aataatatgt aagtataggt aaggggtgtt tttatattta taggttaaga aatttaaagt
    14941 aaattttgag ctggacttga ttgtctataa ataattgatg ggtatataac ttgttgctga
    15001 ataccattta tacgcccagt tactgtatca ataatcatcc taagactacg ttctgctgtc
    15061 tcttccattt ttaaatctat aactgtcaaa ggaggaatac ttcttgccat tagtcgtgaa
    15121 ggtccattac ttgctgcaaa tatttgaata tcctctccaa ttacaattcc agattcatga
    15181 aatgcattca tcataccaag agctacaaca tcatgattac aaaaaattac tttttttagt
    15241 gaattttttg caagcatttt ttgagcaata tcatatccac tatctatttt attagaacaa
    15301 taatatatag aatctgatag atgtattcca tgttctttac atgtttttaa aaaagattta
    15361 ccccttaaag tcatacatgt aaaggaagtt gggttgaata caacagcaat atcattttta
    15421 ccattttgaa tagcatgtaa agctgctata cgaccagttt cctcattatc aatagataca
    15481 cttgaaaaac caggtatttc acgatttaca acaactacag gaattttagg tttatttttt
    15541 actagatatt ctatatcatc agcattgata cctgttaaaa caattccatc ataataatta
    15601 tttgcaaaaa agtattcatc atcacataga tgaccaactt tgtaaggtcg tatgatgata
    15661 tttatgttta aatgttctaa agaaattatg tggtttattg cttgaagtat attggttgat
    15721 gccaactcaa atccctcatc ctgccaataa aaagctattt taatattatt atgtttaata
    15781 ggagtagagc gtttatatcc aagcttatca gctgaattta atataagctc ttgtgtatgt
    15841 agcggaatcc tgcgttcatt tcctttacca cttaagacgt acgagacagt tgctgaagaa
    15901 agaccacagt cttttgcaat atctttgatt gttgccatga taaaatcctc cttaaatagt
    15961 tgcttaaatt tgcttatacc aataatctta acatatattc aactaaagaa caatattcta
    16021 caaggtgcat attttacagg attagaataa ataaatttaa aaaaacttct tacttatgtt
    16081 gacataattt agtttatagg attataatat gcttaaataa gcttattatt aactcaaatt
    16141 aagcaaagtt aagcaggaac atgcaatgct agcttgcaat gtgactgtaa agggggaaaa
    16201 ggtatgagta aggtaaatat atttgaagtt ggtcctagag atggatttca aaacctgaaa
    16261 gagtttttag aaactgataa aaagcttaag attattgatg gtcttattgc ttcagggatt
    16321 aaacaacttg aaattagttc atttgtaagc ccaaaagcaa tacctcagat gaaagatgca
    16381 aaagaagtag caacttattg tgtagaaaaa tatcctgatg tgaaacttta tgcattagtt
    16441 ccaaatttat atggtgctaa agttgcatgg gagtgtggta taagagatat atcctatgtt
    16501 atatctttga gtgaaactca taataaaaaa aatataaatc gtacccatga agagtctatt
    16561 gatgagctta aaaaaataat gaatacttat ccagacatga atatatgtat tggaatggct
    16621 actgtatttg ggtgtccttt tgaaggttta ccaaaaattg aagatgccat aagatttgct
    16681 aaacgccttt gggaaaacgg tatcagggag ataaatctag cagatacaat agggattgct
    16741 gaccctaagc aagtaagaga aactattagt gcattagttg aagctttacc agagtgcaga
    16801 tggcaggtac atattcatga tactagaaat atgggaatgg ttaatactct tgtggctatt
    16861 gaatctggaa ttactactgt tcagtctaca cttggaggat taggaggatg cccatttgct
    16921 cctggagcat ctggcaatac tgcaactgaa gatttagtat atatgcttga acgtatgggg
    16981 tatgatacag gagttgattt tgttaaactt cttgaattat caaagtatca gaagagtttt
    17041 gtaaatggag tatatagtgg tcatcatgta aatataaagt ctgatagttg taattgttag
    17101 ttctattgat taatcgatta aaaaaatatg ttgattaaaa aaatatgctg attggtaaaa
    17161 tagatttatt gataaaaaag ataaattata aaatacatta tctaataaaa caaataaaaa
    17221 aattgagggg gattagtatg gatattatag ctttactttt atttttagga gttattatta
    17281 ttgcatttgt acgtaagaat aatgcaggta ttttggctct agctattggt gtcatagctg
    17341 ttagaatttt tggaatggat gataaagaac tttatggagc tataagcaac tcaatgttta
    17401 ctacacttgt tggtataaca ctactctttg caatagtgaa ctcaacagga gcattaaagc
    17461 ttgttgcaga aaaaattgta gctgcttctg gtaaacgtgt ttggcttata cctatagcag
    17521 tttttatagc aggatttgtt gttgctggtg ttggacctgg ggcaattcca gcacttgcaa
    17581 ttatcccagc aatggctgtt cctgtagcca taacggttgg ttataatcct gtaatgcttg
    17641 cacttatagg tgaatgtgga ttaatggcag gtcgtatgac tcctattact ccagaaggtc
    17701 aaattataac aaccgttgca gaaggggttg gaattgaaaa tgttatgcct actatcttag
    17761 cctgtcaaac tctttctaca attgtatttt caatagtatt gtttataatt ttcaaaggat
    17821 ataaacttaa aaaacctata aatgttatag atagtaagca aatagaaaaa tttaatataa
    17881 agcagataat ttctctaata agcatagtag ttatgattgc ttttatcata atttttgata
    17941 tgaatgttgg gcttgcagct tttgcagttt cagctgtact tatttttttc aatattgctg
    18001 atgaaggaga atgtatcaaa tcaattccat ggagcacgat agtaatggtt cttggagtag
    18061 gtgcaatgat gacaatcgta gatgcagcag gaggtattga cttgatgagt aatgcattat
    18121 catcactgat gaatagtaaa acagctactc caattatgag tatttcagca ggacttatgt
    18181 ctatggttag ttctgcttta gcagtagttt atccaactat gatgcctatg tgtgttgata
    18241 tagctaaggc agttggagga gtagacccac tagcacttat tgctgcagtc ggtgttggag
    18301 gttctcttgc tggtgtaagc ccactttcta ctggtggggc attgatttta gcagctatgg
    18361 gttcaagtaa gaaagacttt aataaggaag aacaaaataa ggtttttgta cagcttttca
    18421 ttatgagtgc agttggctta ttggttattg cagtagtatc aatattatca tttaatgcaa
    18481 taacacattt aatgaactaa ttataataac tgaaaataat tcattaaatt taatttttaa
    18541 tattaatcaa tataatataa tttggagggg aagttatgaa aaaaggagca ttaagcaata
    18601 taaaggtact tgaccttaca agagttcttg caggtccata ttgtacaatg atgttggcgg
    18661 attttggagc tgatgttata aaaatagaga tgccaagaaa aggtgatgat acaagaggtt
    18721 ttggaccttt tgtaaatgga gagagtttat actatgcaaa tataaataga aataaaaaag
    18781 gtatcactct aaaccttaaa tcagaagaag gaaaaaatat atttttagaa atggtaaaac
    18841 atgctgatgt tgtagtagaa aattacagac caggtgtaat ggaaaagtta ggtctgggtt
    18901 atgatgagtt ggctaaagtt aacaatcaaa taatttatgg agctgtatca ggatttggat
    18961 gttatggacc atattcagaa cgtccaggtt atgatattat tgcacaggca atgggtggtc
    19021 ttatgagtat aacagggcaa gaaggaggag aaccaacgag agttggtaat gctatgggtg
    19081 atgtactagg tggaatgaat ttaactattg gtatactcac agcactcaac gctagaaaaa
    19141 taataggaaa aggacaaaga gtagatgttt ccctcgttga ttctgttgta tcgtctctcg
    19201 aaactggtat acagagatac tttgattcag gacaacctga acttatgggt aatagatatg
    19261 cttcagccta tccttatgac tcttttaagg caagtgatgg aagttttgta ataggctgtg
    19321 gtaatgacaa actttttgaa aagttatgta aaaaagttct ttttagagaa gaccttttaa
    19381 tagatgagag atttaaaaca aatcttaagc gttgtgaaca ttacaaggct cttaaaccag
    19441 aaatagaaaa atggtctgtt aagcatacaa taaaagaagc agttgactta atatcagagg
    19501 ctggagttcc tgctgctcct atctataatc ttgattgtgt tacaacagat gaacatatag
    19561 caaaagcaag agaaatgatt gtagatatta gtcatcctat tataggaaat atgaaagtca
    19621 atggaaatcc agttaagttg atgacaacaa aagctaaaat tgatatgcca gcaccatctc
    19681 taggggaaag taacgaggag atttataatg gttggcttgg ttttgatagt gataaaataa
    19741 aagatttaaa agaaaatgga gttatttgat taaaaaatag ctaaacacac ttgtgacttt
    19801 agtcatggga ggttcagtta gaataatatc aaaaaatatt aaatcaatac tgtaaatcaa
    19861 atatagaatt gtcagaagca tttaagatgg gaaaagctac ttagacattg agtctttgtg
    19921 gcttttccta ttttcattaa gaactttata ttatcagaat agatatgtac taaactaaag
    19981 tattaacaat ttaaattaat agaaaataaa ctacctaatt atgttgaata aagactactt
    20041 tagtgaaaat aaacttatag agatgctatt taaattattt taatggaaat atcaaaaatg
    20101 agactgcatt ctcaaaaatg agactgcgtt ttttaagtat tttctcgaaa atgagacaaa
    20161 aatattgttt tatgagtttt attgaagaaa aattcttctg tgaattacaa tttaatcaat
    20221 tttaaagttg aaaattatta aaaatttagt gaaatttatg tatatttcgt ttattttatt
    20281 aaaaataaca attttttact aaaaataaag aacttcaatg gtgattggca tagtacttgc
    20341 tattttatat taacaagaaa tgtagaaaaa ataacaatgt ataatggggg gaatatgatg
    20401 gaatctgtaa tggaaaattt gaacaatctt aaggtggagg ttaaggacaa agtctgtgtt
    20461 attactataa atagacctaa agctttaaat gctcttaata gtgacactct tagggaacta
    20521 tcacaagtta ttgatgtagt atcagaaaat gaggcaatat taggagttat tattactgga
    20581 gaaggtaaag tttttgtagc cggtgctgat atacgccaga tgcaaaacta taaaagtgag
    20641 gaaggcagaa aatatgcagg ttatgctcag ggaatttttg ataaaattga agctcttgag
    20701 aaaacagtaa tagcagcagt taatggatat gcattaggtg gtggatgtga acttgctatg
    20761 agttgtgata ttagaattgc atcagaaaag gctatatttg gacaacctga agttaattta
    20821 ggagttattc catgttttgg tggaactcaa agattatcaa gacttgttgg aactggaata
    20881 gctaaagagt taatttttac ggggagacaa gtaaatgcag aagaagctaa atctattggg
    20941 cttattaata aagtggttcc aagtgattta cttttagaag agtctatgaa gatgatgaac
    21001 caaatagttg aaaaagcgcc aatagcaata agatatgcaa aagttgttat taacaaaggg
    21061 atagatatgg atttaaagaa tgctcttgaa ctagagaaag atattgcagg tttaactttt
    21121 gctacaagag ataagcaaga aggaatgaat gcatttatag aaaagagaaa gccagttttt
    21181 gaaaataagt agctattata actaaattgg aacaatgtag aatatattca aaatctaaat
    21241 ttcgtgaaaa aatacatata gtttaatttt aaaaggagtt aatttatgaa tacgggagta
    21301 attataagtt tagtagggat tatcatagct ctatctcttc tagttgtaat ggttatgaag
    21361 ggaataaata tttttattat agcaattgta tgttcatcat tagttgctat aacaggaagc
    21421 ctaaatgtgt atgatgcact taaagtggat tatatgacag gatttgttgg atttcttcag
    21481 gcaaatttct ttattttcct aacagggaca ttaatgggaa aaataatgga gattactggt
    21541 ggagcaaaat ctattgctaa gatgattgta agatggattg gtaaggataa agcactactt
    21601 tctattccaa tagcttgtgg aatattagcc tatggtggag taagtgtttt cgttgtaagt
    21661 tttgcagtgt ttccgatagc tttagaaatt tttaaagaag ctgatttgcc acgaagattt
    21721 ataccagcag ctttgacttt tggatgctca acttttgcta tggttgcacc aggggcacca
    21781 caaattcaga atattgttcc agcatcaaca ttagggacag atattatggc cggagcagta
    21841 aatggtttta tatcatgtgc agtaatgttt atagttggaa gtaccatttt gtacaaaatg
    21901 gtttcaaaag aaaaagccaa tggtggtcat tttatagcac atgaatcaga tatatttgat
    21961 gaagcagaag caactaattc taagagagaa aatggtccta atggtgtaat tgccttaatt
    22021 ccattgatag tgtcaatttt aataattaat gtaaaagtta atggaaaagc gatagtacca
    22081 attgaggtgg gagtatttat tggagcttta ttagtatatt tattgttaaa taaatatcaa
    22141 gataatacta aaatagttgg acatgttggt gatgcatgta aaactacagt agtagcaata
    22201 tgtaatactt gtgctgtagt tgcatttggt tcagtagtaa aatcagcagt tggttttgac
    22261 ttcatagtta atgccatgac taatattcct ggtcctccta ttgcaggggc tgcagttgga
    22321 actacagtta ttgcaggtat ttgtgggtca gcttcaggtg gactaggaat tgcagctcca
    22381 cttcttggac cagtattttt agccaagggt gtttccgctg cagcattaac taggactatg
    22441 gcaatttcat cagcagcatt agattcactt ccacacaatg gatatattgt tactgtaaca
    22501 aatggtctgt gtaaagaaac acataaggat gcctatatgc caatattctg ggtaactgtt
    22561 ttaactccgt ttattggaac catagtagca gtcctactgt ttacaatgtt cccgggattg
    22621 ccataagaga aaagataaaa ggtataaatt atattttgat attataaaat caattttcaa
    22681 aatatataca ataaatttca tgttttaata ctataaatat gtatttaaaa taacaaattg
    22741 accatataag gaggaattga aagtgttaaa taaaccatta gagggagtta aggttattga
    22801 cctaacgtat tttgtagcag gtccagggac ttctaaaata ttagctgatt ggggtgctga
    22861 cgttattaag gtagagccaa gctttggaga cccaggaaga aagacagggg caacaatgac
    22921 aatgccaata gatgattata ataatccttt ttacagcaca tataattcaa ataaaagagg
    22981 tttgtctata aatcttaaga gtgaaactgg aattgaaatt atggataagc ttttaagtga
    23041 agcaaacgta tttgtttcaa gttatagaac aggagcatta aaaagactag gtcttgatta
    23101 tgaatcatta agtaaaaagc atccacacct tatctggggt cagataaatg gttttggcga
    23161 ttttggacct gctaaggata atgcaggatt tgatacagtt gccttttggg caagaagtgg
    23221 ggctatgctt gatatagcag aaaaagatac aagtccaata aatccaccta tagcctttgg
    23281 agatgcaaca acttcttgta gtttatctgg aggaatatgt gcagctttgt accagcaagc
    23341 taagactgga aaaggacaaa aagtaatggc atctttattt gggcaagcaa tatggaatgc
    23401 atcatcttta ttagcatcta cacagtttag tgatgagtat ccaaaaacta gaaaaaatgc
    23461 tatttcacca gtaataaatt cttatcgttg taaggatgga aagtggatat tcttaagtat
    23521 tttagagcat gaaagatatt ttgaggcact ttgtagatta tttggaagag aagacttaat
    23581 aaaagaagag aaatttgcat cttcagtaga aagtaagaaa aatgctacag aacttattaa
    23641 tattttggat ggtgaatttg caaagtttac tcaaaatgag atggttgaaa aacttactgc
    23701 tgctgatata gctcatgaaa aaattcaaca tgttaatgat gtagtgactg atgaacaggc
    23761 tattgctaat aactatatat atgagcatac aagtaaaaat ggaaataaga caatgcttgc
    23821 atccactcca gttaaatttg gaaatattga agttaacatg acttgtgatg ctccacttat
    23881 aggagaacat tcagatgaga tattgagaga acttggatac agtgattctg atatagaagc
    23941 tctaattgaa tctggaattg taacaatgaa taaagaagca tgtgaaatat gttaataaac
    24001 atgtgaagta tgttaataaa taggagggaa ttttatggat tttagattaa cagaagcaca
    24061 attaatgctt caaagagtag caaaagaatt tgcagagaat gaaatagcac caatagctgc
    24121 tgaaacagat aaaacaggta tttttccaag ggagcttttt agcaaaatgg ctaaaatagg
    24181 atttaatggc attggaacac ctgtagagta tagtggttct ggagggacag atatagaaaa
    24241 ggtaattgtt gttactgaga ttgccaaaaa atgtgcagca tctgcagcaa tattatcaat
    24301 acacacaata tatgctcaag ctattttgaa atttggtact gaggaacaaa agaaaaaata
    24361 ccttccaatg atggctgaag gtgggtgtgt aggtgctttc gccttaactg aacctaatgc
    24421 tggttcagat gctgcaagag ctgcaacaac tgcaattatt gatgaagaaa cggatgaata
    24481 tgttttaaat ggaactaagt gttttatttc tggtggaggt caagcagaat cattaataat
    24541 atttgcactg acagacccat caaagggaat aaagggaatg tctgctatca ttgtcgacaa
    24601 aggtactcca ggtttttcaa taggaaaaat tgaagaaaaa atgggtattc atggttcaga
    24661 aactgcagag ttaatatttg ataactgtag agttccaaag tctaacttat taggaaaaga
    24721 aggcaaagga tttaatatag ctatgacttg tttagatgga gcaagaattg gaattggagc
    24781 tcaagctgta ggaattgctg aaggggcttt agaagaaagt atcaaatatt ctaaagaaag
    24841 agtccagttt ggcaagccaa tttcagcgtt gcaaggtatt caatggtata tagcagatat
    24901 ggcgacaatg gtggaatcag caaaattatt agtatattat gctgcagatt tgaaagctag
    24961 aggagaaaaa cacactaaag aagctgctat ggcaaaatac aatgcgtcaa gtactgcaag
    25021 agaggttaca aatctagccc ttcaaatcca tggtggatat ggatatatga aggattatcc
    25081 attagagaga atgtacagag atgccaaaat aacagaaatt tacgaaggta cttcagaaat
    25141 acacaaagtt gttatttcaa gagcagtact aggataggag gaaaagaatg aatatagttg
    25201 tttgcttaaa gcaagttcca gatactaatg aagttaaaat aaataaagag acaggtactc
    25261 taataagaga tggtgtacca agtataatca atccagatga tagaaatgct ttagaagaag
    25321 ctctaaaaat gaaagatgaa ctaggagcag tgattaaggt tataagcatg ggacctcctc
    25381 aagctaaaag tgctttaaaa gaagctttgg caatgggagc tgatgaagct tatttaatct
    25441 cagatagagc ttttggtggt tcagatactt gggctacatc tacaattatt gctgctgcta
    25501 tagaaaaggt tggaaagtat gatgtaatat tctgcggaag acaagctata gatggagata
    25561 cagctcaggt tggaccagag gttgcagaat ttttagggat accacaagtt acttatgcaa
    25621 aagaagtaaa ggttcaagat gacaaattac tagtaactcg ttatacagag acaggggatt
    25681 atttaataga agcaaagatg ccagttttat taactgctat aaaagagtta aacaatccaa
    25741 gatatccaag tgtaaaagga atcttagaag cttataataa tggagatgcg aagattactg
    25801 tattaacact tgcagattta gatgttgata caactcaaat tggattaaag ggttcaccta
    25861 caaatgttta taagtctttt gcaccagtta aagacaagca taatgaaata atagaaggta
    25921 taaataaaaa agaaaaagct gagaaactaa tagaaatact attcgatttg aaactagtat
    25981 aggagtgtta agatgatgag agcaaaagtt aatcaaggta taaatttaaa tgattataat
    26041 ggtgtttggg taatagggga gcaaagggaa ggcaaaatta atcctgttac gatagaactt
    26101 attggtgaag gaagaaagct tgctgaccaa ttaggaaaag agcttgaagt tgtgatagct
    26161 ggatatgaag tagagaaaga agtgaaggaa ttacttcact atagtgttga taaagtatat
    26221 tacatcaatg accctcttct taaagatttc actactgatg gatatgcaat ttcaattgca
    26281 aatttaatcg aaagaaaaaa gccagaggtt gtattggttg gtgctacttc tataggaaga
    26341 gatatagcgc ctagaattgc aggtaaggtt ggaacaggtc taactgctga ctgtacaaaa
    26401 cttgaaatag attcaacaga taataagtta ttacaaacaa gaccagcatt tggtggaaat
    26461 ttaatggcca ctattgtttg tccaaaaaat agaccacaga tgtcaacagt tcgtccagga
    26521 gttatggcaa aagctgtaag aaacgaatca gagactggaa ttttagaagt tgttactcca
    26581 gaacttactg aaaaaatgat tagaactaga ttagtagagg tacttccaca agaaaagaaa
    26641 tctgtaaatc taactgatgc tagaataata gtatcaggag gaagaggatt aaaaagagca
    26701 gaaggatttg agcttataaa agaattggct gataaattgg gggcagagat tggtgcttca
    26761 agagcagcag tagattcagg gtggatagaa cattctcatc aagtgggaca aacaggaact
    26821 actgttagac cagaactata cattgcatgt ggaatatctg gagcaataca acacttagca
    26881 ggtatgagtg attctaaata tatagttgct ataaataaag atgcaaaagc ccctattttt
    26941 agtatatgtg actatggaat agtaggagat ttatatgaaa ttatacctga aatgatagaa
    27001 tcattaaata ggtaactttt agaaaacatt gaatatgtag aatgataagt cttataatat
    27061 tgtattgtaa gacttattat ttttgctgaa ggttatagtg gggagatgaa acaatgaaag
    27121 agcagtggta taaggatata tttgcaagaa tattatctat gactgatgat ggttttattg
    27181 ttgtaaatac aagtggagta ataatagata ttaatgataa atattgtgac tttttaggaa
    27241 aagaaagaaa agatataata ggacaaaata tacaaagtat tataccaaat acaaaaatgc
    27301 tagatgttat gaaagataag tactgtgaag aaggagctat acatcattat tcaggtggaa
    27361 atacaaaaga aaaaagtgta atagtaagta ggtcttatgt agaaaatgac aatggagaag
    27421 tagttgcagg agtagctcaa gttaaattta gattgcaatc atttgatgta gctaaaaaat
    27481 taatgtcaga atatatggag ctccaatatt ataaagagca gtttaaggat aactgcggtt
    27541 ttgacaagct cataggtgaa aatagagact tcatagaact taagaaaaca ggtgtgaaag
    27601 cttctaaaac aaactttcca gtacttttaa cgggtgaaac aggcacaggt aaagaagtct
    27661 ttgctagagc aattcacaat aatagcagta ggtcagataa acctatggtt agtataaact
    27721 gcgctgcaat accagaagaa ttattagagt cagagctatt tggttatgac gaaggagctt
    27781 ttacaggagc aaaaaagggt ggtaaaaaag gtaagttttt agttgcaaat aatggtacaa
    27841 tctttttaga cgagataggt gacatgcctt tgaccatgca agcaaaactt ttaagggttc
    27901 ttcaggaaag tgagatagaa ccagtgggag gtcttaaaac tataaagata gatgtaagag
    27961 taatctctgc cacaagaaag aatttaagta aaatggttga agagggatta tttagagaag
    28021 acctatacta tagattaaat gtaataaaca ttcatatgat ggaattgaag gacaggcaag
    28081 atgatatttt attgctagca aattatatat taaacaaatt aaatgttgaa tacaaggaat
    28141 tgaaggtttt aagtgataag gttaaaaatt gttttattaa ctatacttgg ccgggaaata
    28201 taagagaact acaaaatgta ataaaaagtg cctatgctgt aagtgatgat atggtcatta
    28261 tgatgtgtga ccttccatct aagatggata atatcagcag agtagctcaa tgtaatgtgg
    28321 atagcaattg ttcaattcat gaaatggttg agaattatga aaaaagctta attattgatg
    28381 tgttaaggaa gtataattgg aaatgttcta aggcagcaga ggttatggga atacacaaga
    28441 gtcttttata taagaaaata aaaaagtatg aaattgaatt aaataattaa gataacttga
    28501 attaaggcaa tgctaaaaaa agcgccctta ttcaaagcga actgtataaa ctgttttatg
    28561 cagttaaaac gtgtaaaagt agatgtgaag tactaatgtc aatcattagt actttttatt
    28621 gtatttataa ttactatcta tatttcagta tcttttgata tctaagatta ttatgtttaa
    28681 tttaggatat aacaaaaccc tcttagttat tgacagtttt ttatatttta actgtctact
    28741 aagaggggat agtttagttt aaatttagag taaattttat ctagcattta taaaaaatat
    28801 tttgaatagc ttttttgtta tttagtcata attttctact tctttatcaa gttctttgcc
    28861 acaagaatca cacttatata tagcattgta tccataatca tattcatcaa ctattttctt
    28921 ttgtctaaat acaccaccac actcacatag gaaaccaatt tttataagtg taacgataaa
    28981 ttgtgatttt ataaatttta tatcattaca tttatcgtta gtgtaaaatg tataaatata
    29041 agagttatcg cactcatctc ttaattcaaa tctttcaata tatgtgttat tttttaattc
    29101 ctcaaattct tctttagtta aatatgattt ttgttgcaat tcatgtaaac gcttaatttg
    29161 gtccattatt tatatcctcc caataatttt agtattttaa gcttagataa ggaatctaag
    29221 ttttattaaa tttatcccaa ggaattattt aaatcatatt tagttttatt aattacaaca
    29281 tatataaatt acacgaaaat aaaactaatt gtaaaaataa tatcctgtga aaacctaaac
    29341 tactaacccc ttactaaccc gaaaactaaa aaaagaaaat atatccaaat ataagtgata
    29401 tataaataaa gatgttttat ataaatttaa ctttaaatat tctgacaata atatatataa
    29461 atatattata gttaaaatat accacatatt ttgggcattg tatactggaa ttagagtaat
    29521 taatttattt tttaacatac aaatgatttt atgaaaaaaa acaagactca aaaattttta
    29581 tattctttga tagaataaaa ttgagagtaa atttattgaa ttttataaaa tttataaggg
    29641 gtgagtctaa gatatgcatg aagataaaaa tgatatattt gaatcaatag atactgagga
    29701 ttttgataat aaaacagaag aagaatgttt agagctagta agaaaaaatg gattgaattt
    29761 aatatacata aaaaatcaaa cagaagagat atgtctagag gctgttagac aaaattgtaa
    29821 tgcaataaaa tgtgttcaga atcagacaga aaaaatatgt atagaagcag ttaaacaaga
    29881 ttggagaatg ttagaatttg tggaagagtc aacagaagaa atttgcatgt tggctattaa
    29941 ccaagatgga acagcattaa aatatataga aaatcagaca gaggagttat gcttaagagc
    30001 cgttgaaaaa aatggtgcgg ctttggagta tgtaaaagaa caaacagaag aaatatgtat
    30061 agaagcagtt agaaacagcg aatttggatt ggcaagagtg aaaaatcaaa cagaaaaaat
    30121 atgtatggaa gcagttaagc attgtagcta taatctaaaa tgggtagaaa atcaaacaga
    30181 agaaatatgt atggaagcag tgagacaaaa tgggcttgat ttgaaatttg taaaaaatca
    30241 aacagaaaca atctgtttaa gagcagtaag acaaaatgga atggcattag aatttgtaaa
    30301 ggaacagact gttggtatat gcttaaaggc agtaagacaa tatggaatgg cattaaaatt
    30361 tgtaaaagaa caaacagaag aaatttgtac agaagctata aaacaagata aaagagcact
    30421 tagttttgta aaaggagata aggaaaaata caaggctcta tatgataata atgaaccatt
    30481 tgcaaagaga tatgttagaa atgtaataga aaaagaaaac agagcattga ttaaaaaagg
    30541 agaagaaaat gcaaaaatca aaattgcagg aaaactttat gatagaggca tgtcttttga
    30601 agatatatca gatatagttg agattgaaat aagcaagtta aaagtgagtt taggtgtttt
    30661 ttaaagtaat aaatgtaggt gttttttatg ggaaatagtt aataaaaaaa taatcgacta
    30721 gctctttgta ggacagcaaa attagtgata taatttgatg tataataaat tatttttatt
    30781 agataagggg gaattaaagt tgaagattac agtattatac caaacaagaa gtggaaacac
    30841 agaaagggtc gctaagctga tagaagatgg agctaaaaag gtggatggag tagatgtaaa
    30901 gcttatgaga cttgataata ttgatttaga ttatttaaat gaaagtaaag cagtaatatt
    30961 tggaacacct acttatttag caaatacaac ttgggaagta aaaaaatggt ttgatgaaga
    31021 ttcaaaaaaa gttaatttag ctggaaaatt aggagctgtt tttgctacat gtgattatat
    31081 atgtggaggt ccagatgttg cgatacttac tatagtagga catttgatgg ttaaagggat
    31141 gcttgtgtat tctggaggtg gttctttagg aaagccattt atacatttag gacatgtaca
    31201 ttcaattgaa ggtccagagt tacaagatga gaaggctata atatttggtg aaagaattgc
    31261 tactaaggca aaagaactat ttgcttaatt tctatatgca taataatgat tagagtaaat
    31321 ttagggtttt aatggagatt tgaagtaaaa gttataaaat aaaaaatttt ccataaaatt
    31381 actcaagatg aattataata taattataaa taaattatat tattaaaata tttaaaatca
    31441 aacgtcaata gcgagggaag tttatggtat ggcatataga atatgagatt ttttctgcaa
    31501 ttatagttat ttttttaatg gtatactttt ttagaagtaa atttatacct actttacaaa
    31561 acaaaatata ttgtgcactt ttaatattct catttttatt tatactttca aatattttag
    31621 gttcattttg tctaaataat atagacaaaa ttccaatttt tattactttt ttattgaatc
    31681 agatatactt attgttattg ccaataccag cagctcttgt atcattttat gttatggcta
    31741 ttatttatca agatataagg tatatgaaaa aaagattatt atttttatct ataccactta
    31801 taatttgtat aatcttatct attacaaatc cttttacgca tattctattt ggattgtcaa
    31861 aagaaacagg ttatatacgg ggttgtggat atgttgcaac ttttgcatct tttttctttt
    31921 atgcaatata ttcagcattt ttatctttta aatatagaaa agcaatgcat gaatcaaaaa
    31981 tatgggctat tagaggtttt cttatagttt caattgtggc aattatctta caggcaattt
    32041 ttttacagta tcttcttaca ggaactgcat gtgcatgtag tttacttttg acatatcttt
    32101 ctatgcagaa tcgaggtctt attattgatg accttactgg tgtactcaat agacaatctt
    32161 ttattcaaga gctagatttg aatatcaata ctgaacaatc aggatttatt attacaattg
    32221 cgcttgatga ttttaagttt atgaatgaaa cttttggaac aaaaaatgga gatattgcac
    32281 taaaagaagt tggaaaatat ttgattcaga tacatgatgt aaaccatgta tatagaatag
    32341 ggggagatat attttctatt gttgtcaata gtcaattagg tatagaacca gatgatatta
    32401 tcagaaagat agaagaaaga tttaaaaagc cttggctagt agaagagata agttttaatt
    32461 tagcaactag tattgctgtt gtttattacc cagaaaatgc agaaacgaca gaagatgtaa
    32521 ttgttgctat agatttctct atacatgaag caaaacgttc tgagtcaagg cgagttgttt
    32581 atgcagatgc gtctataagt gaaaagataa agaagaaaca tgttattaaa aattgtttaa
    32641 aagaagcatt aaaaaatgat gggtttgaag tgtactacca acctatattt tctaatagca
    32701 ggggcaggtt tacttcagca gaggcattac ttaggctaga acacaaagag ttaggtttta
    32761 tacctcctga tgaatttatt ccaattgcag aacaaacagg attgattaac tcaattggat
    32821 tagtagtctt tgagaaggta tgtagattta tagctagtga tgagtttgaa aatttaaaat
    32881 tggataatat agctgtgaat ctttctgtag tacaatgtat gcaaaaaaac ttagctgatg
    32941 atttacttta tcttatgaat aaatataatg tatcaccaac taaatttaaa cttgaaatta
    33001 ctgagactgt ggctatgggc tcttttaata taattaaaga aacaatggaa agattaattg
    33061 atttaggtgt taagtttgca cttgatgatt ttggaattgc ttattctgga gttactaata
    33121 tgctttcttt accattttca gtaattaagt tagataagag tttgatttgg tctatgaatg
    33181 aagatagtag gcataagctt acagttgaga cgattatagc tcttataaat aaattgaata
    33241 tgaaggctgt agcagaaggt gttgaaacca ttaagtgtgc tgaagattta atagctatgg
    33301 agtgtgaata tttgcaaggg tattattttt ctaaaccagt tccagaagat gtttttaaaa
    33361 atttgttgaa agaaaatact tttacaagtt aacacccttt atttttttaa ttacaacttt
    33421 gttcattgac actgccagcg atatattata aaattaacac aataataaat attaaattta
    33481 ttatacatat tattaatatt aattaatgga gtaaagattt attatatttt atttaattat
    33541 cctaaagtga gaaaggaaga tgtgacatga ataaaaagaa aatattagtg ccaattgatg
    33601 ggacagaaag aagtatgcat tctttggaat ttataaaagg aatatttaaa aaagatgaag
    33661 ttgaagtaga aataatgaat gtaaaagaac tagtatttat tgatggcata tccctggctg
    33721 aagagataaa aaattctgaa aatttaggaa gaagaatctt ggataaagca gcagagataa
    33781 tgggagagta tgatgttaaa gtacatttta cttttggata tccaggagat gagataatta
    33841 gaaaagcaaa agaagataat gtagatttta tagttatgac aaaatctact aaaaaaggtc
    33901 ttacaagaat gataggttca gtaacagcaa gtgtagttaa acaagcaaaa tgtatagtga
    33961 tgattgtacc ggagtagaat tagaaaggta tttgaaatat gaataaaaag atagtagcag
    34021 tttgtgaaag tgaaagaagt ttaaattctc ttaagacagc tgcaaatgat ttaggatttg
    34081 atttagttta tgaaattcaa gaaaatgata atataaaaaa tgaactttct atttatgata
    34141 tagaagatgc atctatagtg ttatttgtta caagtgattc tatagaaagc atagagaaga
    34201 ttgatagatt tatagataga gaattttatg aagttgatcc aaagtatata atagaggatg
    34261 caaaatctat agtaagtgaa attttaatag acttaaatta atcgtgggtg tcgcactaag
    34321 aatagagcga cgccttttta catattttac ataagaaata aagggctgtc tcaaactata
    34381 gataagactc tttttcattg ttgaaatgat gaaaatatat ttgatttcat acaaaaaaga
    34441 gtgcctcata actaaaaagt tcactttgag acactccatt tttatatgtt agttgcattt
    34501 aaggtaaaat agaagtttaa tcatgttgaa ataactgaat tacatcttct aaatgattgt
    34561 acattgatgt ataacctaag taagagtctt tactatcaaa tgccatcatt atcttgttat
    34621 ggtggctaat catatcctta agagaatttc tatatttaaa accgaagtat tggttagaag
    34681 aaactgtttt aggtattaac tcataatatt ttataaaaat tggattatga gaagctaaaa
    34741 ttatctcttt gtgaaattgt aagtcagcat cataacattc ttctatcaaa ccatgagaaa
    34801 ccttatccaa ttcctctatt gactttaggt aagtatattt tattttttga agttctttag
    34861 atgtagcatt taaaattgct agtttagtag cttcactttc gataagttgt cttgctctta
    34921 aagattctac ataaaattct tgtatgttat gatttttata gatttcacca tcatcaaata
    34981 ggtcaacttt tttaaatcct tcaactatag gacttgagta aggcacagct ctaagtattc
    35041 cttccttaca gagtatattt atggcatttc ttacaggtat tctacttatg ttaaataggt
    35101 cagcaatctt tctctcactt ggtaatttac tattaatttt aaaagttcca tttagaatgt
    35161 ttaatttaat ataatcaata attttttcag tggcatttaa gcttttcata aaaactccta
    35221 aacatataat ttttttatga ttgtattata tcatagttaa gagttaaatc tactttttta
    35281 taatatgtat aagacaggat agaggccaaa atagctataa tagccaagat aaagtatcca
    35341 tattgtattg aagtatattc cgatatttta ccaactatca ttggaaatac agacattcct
    35401 atagcataaa ttgcttgaaa aaaacccatt gcagtagatt tctgatgttt tggtacattt
    35461 ttcatagact cgctagtaag gtaagaaagg agtatacctg tagatatacc tggtagcact
    35521 tgtagtacaa atattatatt aatatttttt attgttggaa ctaaaaagca ataggctgaa
    35581 actaaaataa aaactaaagg aatccaaaat tttggcccat acttagtaca aaatttagta
    35641 gaagcaaagc cagaagaagc aacagcacac accatgtaag atatagaaga tagacctatg
    35701 agggtagaac tagaacctaa gtttttaaga atttgagtag taaatgacat tgttgtagac
    35761 atttgaatac cctgttgaat taatgccata aaggaaaaaa atacaagtct tttatctttg
    35821 cagatagata aatatttttt aattgatatt ttttcaggta ttttttttga attatgattt
    35881 tttatattta aggctagaat cgttccaata actgaagaca atacactcat gatacataag
    35941 gctgtcattc ctaatttttc ataaaataaa gtactcataa caaatccaat caacattcct
    36001 agattgttta ccaatattat cgtactcgta gctttttgtt gatgttctgt agaaaaaaag
    36061 ttggtatagt gaaccatgta agaaatccaa gttgatgatg atattccaga taaaatgtta
    36121 gctataagaa aaccattgcc tccaggagaa aagactctta taagagatgc taaaccagca
    36181 aagaaaattc cagacagtat aaatatttta tgtttattta tagaatcagc aaatactcca
    36241 acaggaatcc ttaataaaca ttgtgagatt ccatatgcac ctattattat accaatcata
    36301 tttgtagata cattacatgc acttaaatat ggattttgga aaggtatata tacatattgt
    36361 gaaaaccaga aaaatgtaac aatagaaagt aaaagtaatt tttgattaga tatttttttc
    36421 atattatcct ccatatggta tttttataat gcaaat
//
LOCUS       DAGQWR010000021        35546 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.021, whole genome shotgun sequence.
ACCESSION   DAGQWR010000021 DAGQWR010000000
VERSION     DAGQWR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.021"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            100..1119
                     /locus_tag="MIW95_000931"
     CDS             100..1119
                     /locus_tag="MIW95_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBY3150278.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLHGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQAITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            1407..2597
                     /gene="dltD"
                     /locus_tag="MIW95_000932"
     CDS             1407..2597
                     /gene="dltD"
                     /locus_tag="MIW95_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBY3150279.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGINPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            2718..4232
                     /locus_tag="MIW95_000933"
     CDS             2718..4232
                     /locus_tag="MIW95_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBY3150280.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVIFN
                     FSDERNFGDINVIDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            4232..5380
                     /gene="dltB"
                     /locus_tag="MIW95_000934"
     CDS             4232..5380
                     /gene="dltB"
                     /locus_tag="MIW95_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBY3150281.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKHISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            5399..5629
                     /gene="dltC"
                     /locus_tag="MIW95_000935"
     CDS             5399..5629
                     /gene="dltC"
                     /locus_tag="MIW95_000935"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBY3150282.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            5846..6631
                     /locus_tag="MIW95_000936"
     CDS             5846..6631
                     /locus_tag="MIW95_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBY3150283.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            6910..8820
                     /gene="phnW"
                     /locus_tag="MIW95_000937"
     CDS             6910..8820
                     /gene="phnW"
                     /locus_tag="MIW95_000937"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBY3150284.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            9177..9998
                     /locus_tag="MIW95_000938"
     CDS             9177..9998
                     /locus_tag="MIW95_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150285.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            10039..10806
                     /locus_tag="MIW95_000939"
     CDS             10039..10806
                     /locus_tag="MIW95_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBY3150286.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            10814..11434
                     /locus_tag="MIW95_000940"
     CDS             10814..11434
                     /locus_tag="MIW95_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBY3150287.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(11734..12321)
                     /locus_tag="MIW95_000941"
     CDS             complement(11734..12321)
                     /locus_tag="MIW95_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBY3150288.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(12407..12844)
                     /locus_tag="MIW95_000942"
     CDS             complement(12407..12844)
                     /locus_tag="MIW95_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY3150289.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            12948..13946
                     /locus_tag="MIW95_000943"
     CDS             12948..13946
                     /locus_tag="MIW95_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBY3150290.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMTGKHVGRTVVDLEKI"
     gene            complement(14066..14992)
                     /locus_tag="MIW95_000944"
     CDS             complement(14066..14992)
                     /locus_tag="MIW95_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBY3150291.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTVLYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            15378..16982
                     /locus_tag="MIW95_000945"
     CDS             15378..16982
                     /locus_tag="MIW95_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY3150292.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGAANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVLDNNREFIKKYINR
                     FKVGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            17799..19070
                     /gene="serS"
                     /locus_tag="MIW95_000946"
     CDS             17799..19070
                     /gene="serS"
                     /locus_tag="MIW95_000946"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBY3150293.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKHKLNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            19438..20943
                     /locus_tag="MIW95_000947"
     CDS             19438..20943
                     /locus_tag="MIW95_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBY3150294.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            21057..21755
                     /locus_tag="MIW95_000948"
     CDS             21057..21755
                     /locus_tag="MIW95_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBY3150295.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            22040..23128
                     /locus_tag="MIW95_000949"
     CDS             22040..23128
                     /locus_tag="MIW95_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150296.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            23367..24557
                     /locus_tag="MIW95_000950"
     CDS             23367..24557
                     /locus_tag="MIW95_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY3150297.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIEYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYTIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            24648..26150
                     /locus_tag="MIW95_000951"
     CDS             24648..26150
                     /locus_tag="MIW95_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBY3150298.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            26222..27478
                     /locus_tag="MIW95_000952"
     CDS             26222..27478
                     /locus_tag="MIW95_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBY3150299.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            27650..30424
                     /locus_tag="MIW95_000953"
     CDS             27650..30424
                     /locus_tag="MIW95_000953"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBY3150300.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAQALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRSMNSNSKN"
     gene            complement(30489..30674)
                     /locus_tag="MIW95_000954"
     CDS             complement(30489..30674)
                     /locus_tag="MIW95_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBY3150301.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEVYKILQKLDIDFERVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            31046..32224
                     /locus_tag="MIW95_000955"
     CDS             31046..32224
                     /locus_tag="MIW95_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBY3150302.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMFTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIVSIMIKIWLSRFNKFVGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKDMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      32350..32435
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            32548..35502
                     /locus_tag="MIW95_000956"
     CDS             32548..35502
                     /locus_tag="MIW95_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBY3150303.1"
                     /translation="MKKGNKKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEDNSTDNKKPEQTSEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQTPEGEKSIDNKKPEQIPEEDKSTDNKKSEQALEDEKPL
                     DNKNTEKTPEEDNLLEDENLLKVLEEELNEDYGFVVKINNNTIETESMKKISFNLTYT
                     PTSKGIQAGDSITFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGK
                     IGLEAYVKKVDEDTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEG
                     RKSTTVFMPVKNKDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEV
                     SDFNIEQSKNSISVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQED
                     DATVSIFDRGDNYLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGM
                     RLTTSGEDGHDFRVVEVPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTI
                     NLSKEEVESKFFIAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKK
                     EVDKKVLNSSDNQIVKYKINMSTYGVYDAGQVNLLDEVNPSLKISNIKYSDNLELKKE
                     AGDGKNTIRLVNKYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGV
                     ETTKQGYAFEAKKVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGVIQSSVKKPGI
                     YYLVEVMAPSGYEKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPVPPNTDEP
                     IVNPPVPPSTDKPRKPSNPSDTEDTIVINPPVPPSEDIINPPIPEILNPPVPPSEEVI
                     ETPVKQIVPIPEVVKPSVSEEKNNKAKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIV
                     GLVVLRRNKNTN"
BASE COUNT        14117 a         3831 c         6207 g        11391 t
ORIGIN      
        1 ctttcttttt tatttgataa aattatttta taatatatct ataagttgta aagtttttta
       61 ttaaaattaa aataaagtgt agaaaatggg gagtgattta tgatgggtaa aataagattt
      121 ggtattgttg ggacaagcaa tatagcaaat atatttctaa gagctgcatc tagggttaaa
      181 gattttgaat tggttgctgt atattctaga aatttagaaa aagctagaga atttggaact
      241 ctacatggag caagtatttt ttttgatgat ttagaacaaa tggctaaatc gaaggatata
      301 gatgctgtat atatagcttc tccaaatgca ttacattcaa agcaagccat tacttgttta
      361 aaacacaaaa aacatgtgct ttgtgaaaag tcgttagcat ctaatttaaa agaagtcaaa
      421 tcaatgataa aaacagccaa agataataat gtattgctta tggaagctat gagaattaca
      481 tgtgttccaa actttaaagc agttaaagaa aacttgtata aaatagggaa aataagaaga
      541 ttttttggaa gctattgtca gtattcttca agatatgata aatataaaaa tggaattata
      601 gaaaatgcct ttaaaaaaga gttgtcaaat ggagctttaa tggatatagg agtgtattgt
      661 atacatccta tggtgaattt atttggtgct ccaaaaactg taaaggcagt ttctcatata
      721 cttcaaactg gagtggatgg agaaggttca gctgtatttc aatatgatga tatggatgca
      781 ataattcagt attctaaagt agctgattct tatataccat ctgaaataca aggagaagag
      841 ggaagtataa taattgaaaa gcttaatcta tttgaaaaag ctattattaa atacagagat
      901 ggaagagaag aagatatatc tattccaaaa gaaaaagcag aggaaaagcc tagagaaata
      961 gaaggaatgt attatgaact tgtagagttt ataagtctta taaataacaa taaaatagaa
     1021 tcaaatataa attctcatga aaattcaata atagtaatgg aaattatgga tgagataaga
     1081 agacaaagta gtattgttta tccagctgat agtatatagc ttatattaga aaatataaga
     1141 atttataata tatattctta aataaattta attttaagtt aaggtaaggt gtatacttag
     1201 ccaagtgtta aaatgtcaaa ttataagtat gaaaaagtgc taaaaagaaa tttatttttg
     1261 gtgttttttt cttttttttt acaattttgt aacagtatca aaaaaagttt tgacgatttt
     1321 attacaattt tgtagttgaa tatacatatc aaaactaata tgattaataa taacataaat
     1381 ttgaatattt ttagaggaga gaaaatatga gaaaattgat atactttatt acacctttta
     1441 taataggcgt agtattttta tttgggttag acaaatttct tgattctaaa actgatgaac
     1501 ttttgaggga gaaaaatcta cttccaatta tggatgatac tttaagtgat ataaaagata
     1561 aaggggttac agcaaataat cattttttaa gagaaaagga tattatgatt ttggggtcat
     1621 cagaacttag taattcaaca aaacaacatc caaagtacta ttttaataca aataggagta
     1681 aaaataaggt gtttgcaatt gggagagcct atactcagac attacaagat gctgcaatat
     1741 taggaagtat gaatcctaat attgataata agaaagttgt cttgttaatt tctatgcaat
     1801 ggtttatgga aaaagacgga gtcacaagtc accattatca gtctagattt tcaccaatac
     1861 aattttatcg atttttagat aatccaaaaa tatctaaaca aaataagatt gaatatgcaa
     1921 aaaaatcaag taagctatta tggggaagtg atgagtataa agcagaagca ttatatgcta
     1981 agctatatga acctaagaca tttcctgaaa aagctgaaaa ggtgctttta gagccttatt
     2041 ttcaaggaag aaagtactgt atagctctta aagaaaaggg tattctttat aaacgattaa
     2101 ttaagctaga taaaaagaga gcaacaaaaa gaaaaagacc aataaattgg tcatatgaga
     2161 gaaagaaagc cattgaagat gctaaaaaaa gagttggtaa aaatccacta aatattgata
     2221 agtactatta taaagagcat tttaaagatg gtatagagca atacaaagga agagacaaag
     2281 atgtaaattt attaacatct aaggagtttg aaaattataa actgatgcta aatgtatgta
     2341 ctgacttagg tattaatcca gttgttgtat taatcccaag tatggataaa ttctataatt
     2401 tgactggtat ttctgaaaaa gaaagaaatc agtactacga taaagcacaa aaaatagcag
     2461 aaagcaaagg ctttgaagtt ctaaacctta aagataaagg gtcagataag tattatttaa
     2521 gggatgtaat gcatcttggt acgaaaggat gggtagatgt ctgtgagaga ttatttaaaa
     2581 tatttaaaga acaataaaga tttgttaact agtatttgtt taactatggt tataatattg
     2641 gcgataggag ttctaacatt tactccattt aaagaattgc catttattta taacggattt
     2701 taaaggagca ttcttatatg aaaattattg aaggaataaa aaaatattca aatacagata
     2761 gaacagcact aatgtgtaat ggagataaac tttcttataa agatttaaat gaatattcag
     2821 atgctatatc agtatttcta aaggatgtat acaaagaaga ggatactcct atagtaattt
     2881 atggaaataa agaaaacatg ataatggcat gtatgatagg agctttaaaa tcaggtagag
     2941 cgtatgtgcc tcttgatatt agttttccaa ttgatagagt ttttgaagtc actaaagaga
     3001 taaaaccaaa agtaatattt aattttagtg atgaaagaaa ttttggagat atcaatgtaa
     3061 ttgatatgga taaattaaat gatataataa atgaatatca gggaaaatca ttagataaag
     3121 aaaactgggt aaaagatgat gaaaatgcat atatactatt tacttcagga agtacaggaa
     3181 agccaaaagg ggttcaaata agttcaaaca atttagatag cttttcagat tggataagcc
     3241 catatctaaa catagatgga agtgaaaaag taataatgaa ccaaccagca tattcttttg
     3301 acctttcagt tacaacaata taccctggtc ttatccatgg agctacatta ttttcaatat
     3361 caaaagatgt attagcagac tataaagaac tatttagaca atttagtatc tcagatatag
     3421 ctgtatgggt gtcaactcct tcttttgcag gagtatgtat aactgaaaaa gagtttaata
     3481 gtaaaatgct tcctaacctt gagtcaatga tattcatagg agaagcactt tcaaaaaatc
     3541 ttactaaaga acttatgagt agatttccaa acacaagaat tatcaatgga tatggaccaa
     3601 ccgaggctac tgttggtgta agtgtaaatg atatgactca aaaagctata gatgatgaaa
     3661 aaagtcttcc agttggatac cctatgtcaa attgtaaaat aaaaatactt gatgaagatg
     3721 gaaatgaact taaagaaaat gaaaagggtg aaattataat aattggacca tcagtttcta
     3781 aaggttactt caataataaa gaaaagactg atgaagtatt tttctatgat gaaatagatg
     3841 gtgtaaagtg gagagcttat aaaactggag atatgggata tcttttagat ggaaatatat
     3901 attattgtgg aagaaaagat tttcagatta aattaaatgg atttagaatt gaaatagaag
     3961 atatagaaaa taatttgaga aaagtacata atgtaaaaaa tgcagtagtt ttaccagtat
     4021 ataaggacga aaaaattgct tacttaaaag gtattgttga gttaaatgaa aagaatgatt
     4081 taagtaatat taaaaatggg atgataataa aaaaggaact tggaaagtat ataccttcat
     4141 atatgattcc aagaaatatt tcaataatct ctgagtttcc aacaaatatc aatggtaaaa
     4201 tagacagaaa gaaacttatg gaggaaattt aatgattttt tcacagtatg gagattattt
     4261 ttatctttat atattattat taacatcaat accagcagta attttaggtc taatgggcaa
     4321 gaatataaag tattatggta tgttggcaag tttgtttatg atattcttaa tagtaggaat
     4381 tgatgtccag ctaaagtatt tggtaatatt tattattctt gaagtaataa tagttaaagg
     4441 atatgaatat gtaagaagaa aaactaaaaa taaatacata tattggggat tcttgtttgc
     4501 atcaatgctc ccaataataa taaataaaat atctccagtt acttcttttg gaataattgg
     4561 atttattgga atatcatact taaatttcag aactatacaa atggttattg aaatttatga
     4621 tggagctata aaagaagtta aaatttcaaa gatgctttat tttatgttat tttttccaac
     4681 tttaagttct ggacctattg ataggtcaag aagatttgaa caggacttgg aaaaacacat
     4741 ctctagaaaa gagtatatag aagaatactt actgccagga ataaaaaata ttgtcatggg
     4801 agtaggttat aagtttgtta tagcattttt gataaataca tattgggtat caagaatacc
     4861 taaagatatg agttttataa atattttaag ttatatgtac gcatatagtt tatacctgtt
     4921 ctttgatttt gcaggatata gtttatttgc tataggtaca gggtacatat ttggaataca
     4981 agtacctata aactttgaca agccatttat cagtaaagat atgaaagagt tctggacaag
     5041 atggcatata agtctttcaa gatggtttgg agattatatt ttttctagat ttgttatgtc
     5101 atcaatgaga aagaaaagat ttaagaaaag gactactgct gctcatgtgg ctcagatgat
     5161 aaccatgata acaatgggat tttggcatgg tctaacttgg tattatgttg cttatggtgt
     5221 ataccaagga ttggcacttg tgttaactga tatatatcag cgaaaatcta aattttataa
     5281 aaaacataaa aaagataaat ggttcgagag agtgcagatt ttcattacat tccatattgt
     5341 gtgttttgga ttgctgatat tctcaggtta tttagcttaa ttttaaggag gaaaatttat
     5401 gcaagaaaca gttatagaaa tatttgaaga tgtattaggg actgatgaga taagagaaga
     5461 tttagacctt aatttatttg agacggaact tttagactca ttagctataa ttgaagtttt
     5521 acttgaaatt gaaaatagat tagggataga gcttcaacca actgatttag agagaaaaga
     5581 tatgtctact gtaaataatt tagtaaaatt cttggagaca agaaaataaa ttagtagtta
     5641 gactttaaat attaatttat aaaataaaat tttacaaagt ccatctgata ctaatatttg
     5701 atggactttt ttaatgataa tacaaagtct tttgaaaata actttaatta agtaaaaaat
     5761 ttaatacata aatatgattt cataaatctt aggtatcatt gtaatattat aggagaaaaa
     5821 tatattatat ttaggaggaa aatgaatgtc tttaaattcg atagatagaa aaaaagaaat
     5881 acttaaatta ttagattcaa aagggaaggt aaatactaaa gaattagtaa aaagattaga
     5941 agtatcatca gaaacaataa gaagatattt ggaagaatta gaaaatgaaa ataaattaaa
     6001 aaaggtatat ggtggtgcta taaaaataaa ttatggagtg attgaaccag aacatataaa
     6061 gagaaacaat actaacctag ataaaaaaca aagtattgct aaatacgcag ctaagtttgt
     6121 aaatgacaat gagtcaatta tcatagatga aggtactacc aatttgcaga ttgtagatta
     6181 tataatatct gtaaacaatt taaaaattat tacaaactct tatccagttc tatcaaaatt
     6241 gatttcttat gagaatcaag gtttgtttga tggagaagtt atatttattg gtggaaaagt
     6301 aaatttaaaa caccaaagga cagcatgtcc aatgtcaata gacactatga aaaacttata
     6361 cgtagataaa gcttttatat ctagtgaagg tatacttgat agttttggaa ttagtagtat
     6421 agaccaaaat aaggcattgt tgtcaaaaga atatataaaa aattcaaagg aaagttttgt
     6481 attatgtgat agttctaaaa taggaattgc tagtatgtat aagatagcag atttagagga
     6541 attagatttt gttatttgtg atgtagaacc tccgaatgaa tggaaggata agttaaatga
     6601 gataaatata acatggatag aagcaaaata aacccttagt tttatataat acaactatgc
     6661 ttaatacaac taaattcata aaatatagtc agttcataga ttaagattag aaagtaaatt
     6721 ttagattgta acttaggatt ttatggaaat atgatataaa ataaaaacaa ttaaatacaa
     6781 ctaaaaacag aaatttaata tatatttaac ctaaatatga tgtataatta acatatgaaa
     6841 gttatacttt aaaagtaaaa acaattaaaa acacaaaaat tctaaaatga atctatggag
     6901 gctataaata tgaaaaagat atatggagaa aaaataaaag cagtagtatt cgattgggct
     6961 ggaacaacag tagattatgg atgttttgca cctcttaatg tatttataga gatatttaaa
     7021 agaagaggta tagatgtaac aatggaagaa gcaagaaaac ctatgggaaa attaaaaata
     7081 gaccatataa gagaaatgtg tgaaatggat agaataaaaa atctttggtc agataagttt
     7141 ggtaaagtgc caactgaaga tgatgtaaat gaactttatg ctgaatttga gccaatgtta
     7201 tttgagacat tagaagagta tacgactcca attcctcatg ttgttgaaac tattgaaaag
     7261 ttgagaaaaa atggattaaa aataggttct acaacgggtt atacaagaga gatgatgaac
     7321 atagttgaac caaatgcagc aaagaaagga tactcacccg attttcttgt aacaccatca
     7381 gaagtatctc aaggaagacc gtatccttgg atgtgctata aaaatgctga agctttaggg
     7441 gtgtctccta tgagttctat ggtaaaagta ggagatacaa taagtgatgt aaaagaaggc
     7501 gtaaatgcag gaatgtggag tgttgctgta ataaaaggtt caagtgagtt agggcttaca
     7561 caagaagaag tagagaacat ggataaggaa gaattaaaag ctaagatgag tatagtttca
     7621 aagaaattta aagaggcagg tgcacatttt gtaatagaaa ctatggctga gttagaagat
     7681 atactaataa aaatagagaa tgaaactatt aaatcagatt ttgtaccaga aaatgattac
     7741 atattactta caccaggacc actatctact actaaatcag taagagcatc tatgttaaaa
     7801 gattggtgta cttgggatgt tgaatataat aacctagtac aagatgttag aagaagatta
     7861 gtatctctag caacccaaaa tacagataaa tacacaagtg tattaatgca aggaagtggg
     7921 actttttcag tagaagctat aataggaagt actatttcaa aagatggtaa acttcttgta
     7981 attgcaaatg gagcatatgg aaagagaatg aaagatatat gtaattatct taacatagaa
     8041 tttgttgact gcacatttaa agatatagaa gcagtggatt taaatgtagt agaaaattta
     8101 ttaaaagaaa acaaggatat aacacatata tcaatggttc actgtgaaac tactacaggt
     8161 agattaaatc ctatacaaga ggtagggaaa cttgcaaaag aatataacaa aatttatata
     8221 gttgatgcta tgagtagttt tgggggaatt gagattgatg ttgaagattt taatatagat
     8281 tttttagtta gtagttctaa caaatgtata caaggagtac ctggatttgg ttttataata
     8341 gctaataaag agaaattatc taaatgtaaa ggtatagcta aatcattatc tcttgatgtg
     8401 tatgcgcaat gggagactat ggagaaaaat aatggtaaat ggagatttac ttcacctaca
     8461 catgttgtaa gagcttttta tcaagcttta ttagaattgg aagaagaagg ttctgttgaa
     8521 aaaagatatg ctagatataa agaaaatcag tttacaatag cttcaagatt aaaatcttta
     8581 ggatttgata ctttggtgaa tgataatgct caatcacctg taataacaac tttcttatat
     8641 cctaaaaatg caaagtttga atttatggaa ttttatactt atttaaaaga taatggattt
     8701 gttatctatc caggtaaatt gactgatata gatacattta gaataggtag cataggtgaa
     8761 gtttatccaa ctgatatgga gagactagct gatgtaatag aaaaatttat caatagatag
     8821 aagaagatag tgtcttttag gttacagaat agtgcggaga gcctactcct ttaggtgtga
     8881 aatgatagca caaacagtgt ataaaaagta ttgacagtag aatttaaaga cttatatttt
     8941 agaagtttat acaatgaata tataaagatt gagtaatagg gatatatttt atttaaacca
     9001 caatctaaga aattagattt ctagtttaaa taaagcattt ccctattatt tttatgccta
     9061 tttatagaaa ataaatgata gttgctttat taagtattat tatcagactt gacaacgtaa
     9121 caatgttttg ttatattagg tttaaagata aaatacggta aagaaaggat atcattatga
     9181 atgaagaatt aagttcacta agatttgatg gtgttggaaa atgtaaaggt ggaatttttg
     9241 ataatgttaa tatatcagga acaggcaaaa tagaaggaag tgtaaaatgt agtaaatttg
     9301 attcttctgg ttcagcaaaa gtttttggga caacagaatg taatgaattt tttacatctg
     9361 gtttaagtaa agtagaagga aacatactgg catcaaaagt tgagatatcg ggacttctta
     9421 gatgtacagg aaatattaat tctgttgaaa ttgatactag tggaattatt aatgtagaag
     9481 gaagtataaa atcggatatt attcatggag aaggatattt aaaagcaaat gagaatatag
     9541 attgtgaaaa tatcaattta agtggaatct tggagtgtaa tggatttttg aattgtgaag
     9601 aagttaatat tagtttaaat ggaacttcta attttaatga agttggagca agtagtataa
     9661 atataaaaaa ggggcatgga gattattgca aaaaatttcg tatgctagaa aaaaagcata
     9721 tcaactttga aaaagtgtct tcaaatttaa taagtttagg tctattatca attagaaagt
     9781 atacagaaag taaattgttt gcaaatatta tagaagcaga tgaaataagc ttagaaaata
     9841 gtgaagtcaa agttgtaaga ggaaaaaata ttaaaatagg caagggatgt tatgtagaaa
     9901 gtttggagta cagtgaaagt atagagattg atgaagattc aatagttggt gaaataaaga
     9961 atataagtga aaaaataaaa ttaaagaagg ataagtaata aataggttta aaaataaaat
    10021 ttaaaatgag gtaataatat ggaaaaaggg tctataaaaa tacttggaga aggctttata
    10081 agtagtggtg agtatgaaaa tataaaaatc gtgggaagtt ctaaatcaaa aggaaatgtt
    10141 aaaagtaacg aagtaaaagt ccttggagat gcaaaatttg aaggtgacat caatataaaa
    10201 aattttgttg ttaacggaaa aactattata agaggcaatc ttaaagcaga gcgtgtaaga
    10261 gtgaatggtc aacttaatat agagggagat gcagaaatag atgacttagt tataactgct
    10321 caacagaaag tggatggcaa acttaaatgt aataatgtta caataagagg acaattaagt
    10381 gtaaatgaaa atatgtatgc acaagatata aaaatctatg gcgaaatgag aaataaatca
    10441 aatatagaat gtgaagatat aaaggtatat ggagtaataa aatgtgatgg tcttttaaat
    10501 ggagaaaata tatatgttta ttcaggagga ggttcttatt gtaaagagat aggggcaact
    10561 aatataaaaa taggaagaag caaagatgct ttttcattaa ttaaaatcat taatattttt
    10621 tcaggaaagt ttaaatgtaa tttgatagag ggagatacta ttgaattaga agatgcatca
    10681 ataaaagata tacgaggcaa aagcatatct ctagtagata attgtgacgt tggaaatatt
    10741 gaatacagtg ataatttatc agtttcagat aattctactg taaaaaacag tataaaggtg
    10801 ggctagaaaa gttatggaag aattaatatc taagaaagat ttattgagca aaactaatat
    10861 atcttatggt caattatata gatggaaaag aaaaaacata ataccagagg aatggtttat
    10921 aaaaaaatca gcttttacag gtcaagagac tttctttcca agagataaaa tactagaaag
    10981 aatagagtta atattgtcta tgaaagaaga gacttctctt gatgatattg caaatatgtt
    11041 tatgaaaaag gatattaata aggaatttga cattgatttt attctcagta aagaagttat
    11101 atgtaattat accaaagaaa tatttcaaaa tttatataaa aaagagcaaa gcataggtaa
    11161 aaaagaactg cttatattga gcataataga gaagttttta gttaaatcta taataacttt
    11221 tgaagagtta aaattgatag tgaatataat agaagaaaat tttacatcaa tatacaatga
    11281 aagtggaaag atttatttgt ttagaaagtt tggagttcca ttcgtagtag gatgtctgga
    11341 ttataaaaaa gtaaagtttg aaaaagatgt agttaagatt attgagattg acttgacaaa
    11401 agaaataaat gatataagta taaagttatc atagtgtatt atcttatata atgtattata
    11461 ataatctact taaaatacac tttatttaaa tatttagttt aaagataaga ttttatgtga
    11521 gtatgtaaaa gttaaagata gatttaagta ggttataggg attagtaaat aataaaatga
    11581 accatgatga attactataa attgtattta aactacattt tctacttata gtaatatcaa
    11641 tggttcattt tattgttatt aagataatac agtatttata aaaatacttg atataaatat
    11701 agtaaattgt ttaaatagaa ttattgcctt agtttattca aataatgagt ttttaaaatc
    11761 taagaaatat ccttgtggat gaagacatac aggacaagct tttggagctt ctttacctat
    11821 atgtatatgg ccacaatgtg tacatatcca ttgtatatct tcatttcttc tgaataaagt
    11881 accttcttct aaatcagtag catatctacc aaatctatca ccatgaactt tttctatttc
    11941 agctatattt tcaaataact tagctataga ttcaaatcct tcttcacgtg ctgtttttcc
    12001 aaagttttta tatacatcat cccactcttg aagctcatta tgttgtgcag ctttaaggtg
    12061 ttgtgctgtc ttgtcatata aatctacagg gtatgctcct gatatactta tattttcacc
    12121 agcaaattct tttaatttag aaaagaattg ttttgcatgt gatttttctt ggtcagcagt
    12181 gtaattaaat aaagactcta ttatatataa tccttctttt tttgctacag atgcagaaat
    12241 attgtatcta tttctagctt gactctcacc tgcaaaagct cttaataaat tttctttagt
    12301 tttactatct tttaaattca tattctacct ctctatcata ataaattttt acaatatttg
    12361 attcttttta tttattattc caataggaga tataattatg ctcaatttag tcagcatttt
    12421 taaaattatc attaattttt tttaaaattc taagaaattc ttgtttttca ttttctgtaa
    12481 tattatggta tgctgtctct ttaacttgtg ttgagatgct attaaaagta tcttctataa
    12541 gcagtgcctt ttttgtaagc tttatatacg tgatacgttt gtcatttgta catttttgtc
    12601 tttctaaata tcctaggttt ataagtttat taaccaacac tgtaacagtg gatttatctt
    12661 ttcctatttt agctgcaatt tctttcattg taagtattcc attattttca taaagtgcag
    12721 ttaaaatatt tccatgagta ggtattaact cacctaaacc atttttactg agtttatttt
    12781 caatgaattt aatcatacta gcctttgttt tacttatgaa atgtacaata tatttatctt
    12841 ccataaatta ttctccaatc tttaatctat ttaaattagt ttgacataaa actatttata
    12901 aactataatt agtttaacat aaaactaaat gtattgggag gaaatttatg aataaaaaat
    12961 ttaaatcact atatattaca gaagaagaag gaaattttag aagaaatata gttgaaagag
    13021 aagtagatac attgccagaa ggcgaagttc taataaaagt aaaatattct tctctaaatt
    13081 acaaggatgc actttctgca aatggtaata agggtgttac gagaaagtat cctcatacac
    13141 caggaattga tgcatctggt atagtagaat ctagtagttg tgatgatttt aaaattggag
    13201 aagaagtttt agtaacaggt tatgacttgg gaatgaatac ttctggtggt ttttcagaat
    13261 acataagagt tccttctagt tgggtagtta aacttcctaa aaatctttca ctaaaagaag
    13321 caatggatta tggtacagca gggtttacag cagctttatc tgtgtataaa tttatagatt
    13381 ctgtagacaa gaatatggga gatgtattag tcactggtgg tactggagga gttgctgtaa
    13441 ttgcaactaa aatattgatt aaattaggtt attcagttgt agtttcaaca ggaaaattag
    13501 aagaacaaaa agaagtatta ctaaacttag gtgttaaaga tgttatacat aggtcagaag
    13561 tggatgataa ctcaggaaga cctctgctta gaccaagatg ggctggagta atagatacag
    13621 taggaggaaa tactctagca acagcaataa agacaactaa ttattgtgga gcagtaacaa
    13681 cttgtggtaa tgcaggagga gttgatttta caagttcagt atatccattt atcttaaagg
    13741 gaattacttt atatggtata gattctgtgg attgcccaat gaacttaaga ttaaaaatat
    13801 gggatttatt atcaaatgaa tggaaattag atgatttaga tgatatggta aatgtagtat
    13861 cattggaaga gttaaatgat agtatagatt cgataatgac aggaaaacat gttggaagaa
    13921 cagtagttga tttagaaaaa atataggcga ataaatttat aattaggatt ataggtttta
    13981 agcaaaaatg agatacttga aatttactta tgagtatctc atttttttat gtaaaaaaat
    14041 ttatgtagat gtttgtgtaa aaagattatt catgaacaat taattcaaat acatctggtc
    14101 ttgaatagtg accttctgaa tcaaaatcta gtctactaag tactattttt tctaggtcta
    14161 aatcagctat tagcatttct tctttattga aaataggacc tgctacatat tttccaaaag
    14221 ggtcaactat acaacttcct cctggacaca taatttctgg ttctgcatct agctccttat
    14281 agtagttaag gtcagttgga tacatatttt tttcaacata ttgattgcat ccaattacaa
    14341 aacatcttcc ttctaaagca atatgcttca tagttgcttg ccactcttct cttgaatctg
    14401 cagtaggggc tatgtaaagt tttacaccct tagcataaag aactgttcta gctaatggca
    14461 tataattttc ccaacaaatc aacgaaccca tttttccata tggagtatca actactgtaa
    14521 gagtacttcc atctccttct ccccaaatac atctttctgt tcctgtaggt ttaagttttc
    14581 tgtgtttacc aattagctta ccttctggag agaaaaataa gtttgtgcaa taaagggtac
    14641 aatttatatc attaccatct ctttctgtta tacccattga tagatatact cctgcttctt
    14701 gtgctgcttt tcctaataaa tctgttgtag cactaggaac tggtacagaa ttatcatagt
    14761 atcttttcca atcttctcta ccttccattg ttctacttcc aacaacaaat ccaaatgaaa
    14821 gacctcttgg atatgcaggt atgaatgctt cagggaaaac tacgatattt gccccttttc
    14881 taccagcttc tttaattaat cctaatgctt tttcaacagt tttttctaaa tccattatga
    14941 ctggtgacgc ctggacaaca gcaattctta catttttatt ttccttgctc ataaaaaacc
    15001 tcctaaaaaa tatttaaatt ttttgcattg aatagattac atgagatgtt atttgagaac
    15061 taagatactt atattgtgaa taataatgta actgtatatt taaaataata catcatgatt
    15121 taaaaaatta caattgtaca gatataattg agtttagata tttaaattta acaacttata
    15181 atattttttc aattagtata aagcctatta atttgtctta tttacaatga atagtattta
    15241 cttcttggca aacaataatt aaaaataaaa cttttatatg attaaactct aaagaaaaag
    15301 tttataaaga agataatata attaagcaga aaataatgaa aaaaatttat taaagtagac
    15361 aacgtaagga gaattttatg aaagaaaaaa tttattacaa tggaaatatc atcacaatgg
    15421 aagattcaat atgtggagat gctattctta taaaagataa aataataaaa aagataggaa
    15481 caaaagaaga agtatttgct ttgaaaaata aagatacaga aattatagat ttacaaggta
    15541 agacattgat gccatctttt atagattcac atagtcactt gatagctttt gctactactc
    15601 taaagctagt tcctttagaa gatgcaacca gttttaaaga catagtaaaa aaaatacagg
    15661 atttcaaaga gtcgaataat ataaaaaaag gagactggat aattggattt tcatatgata
    15721 ataatttttt agaagaaaat aagcatccag ataagagtgt actcgatagt gcatcttcag
    15781 aaaatccaat actaatttca catgcttctg gtcatatggg agctgcaaat acattggggt
    15841 taaaacagct tggtgttacc aatgaaacac gtgacccaga aggtggacat ataggtagag
    15901 tagaaggcag tgaagaacca aatggatatt tagaagaaaa tgcatttttt aatgtagcat
    15961 ctaaaataaa acaaccatca tcaaatgaga tttttaattc aattgaaaaa gcacagaaca
    16021 tatatctaag ctatggtatt acaacagctc aagaaggttt aatggaagaa aatcaattta
    16081 acattttaaa agcaatggca aatcaaaata aattaaaaat ggatgttgta ggatatgtaa
    16141 atttgaaaaa aagtaagtct gtattagata ataataggga gtttataaaa aaatatataa
    16201 atagatttaa agtaggaggt tataagattt tcttagatgg ttctccacaa ggtaagacag
    16261 catggttgag tagaccatat gaaaattcag atgatggtta ttgtggatat cctatataca
    16321 aagatgaaga agtagaaaag tttatagata tttcactaaa agaaaagatg caactcttaa
    16381 ctcattgtaa tggagatgca gcagcagacc agttgataga tgcgtttgag aaagttttaa
    16441 acttaaaaga gcaaagtagt gaaaataata taagaccagt tatgatacat gctcaaacag
    16501 taagagcaga ccagattgat gatatgaaag taataaatat gataccttca tactttgtag
    16561 ctcacacata ttattggggt gatattcata tcaaaaacct tggagaagat agagcgttta
    16621 agattagccc acttaaaaca actatagaaa agggattaat ttacactctt catcaggata
    16681 caccagtaat agcacctaat atgctggaaa cagtatggtg tgctgtaaat agaattacaa
    16741 aaaaaggtat acaaatagga gagaatgaaa agataagccc attagatgct ttaaagggtg
    16801 ttactataaa tgcagcatat caatattttg aagaagataa aaaaggttca attaaagaag
    16861 gtaaattagc taatttgata atattagatg aaaacccact tacaatcgac cctatgaaaa
    16921 taaaagatat taaagtattg cagactataa gagaaggaga agtattgtat tctttgaagt
    16981 agatgaaagt tagatatttt ataatctata aaaaatagtt ttatataata agttaggtag
    17041 aattaaataa gattacagtt aaatcaacaa ttatattttt tattaaattt taattattgt
    17101 attgaagatt tataagtata attttattga ttctaccttt ttaaataact gtcgatttat
    17161 taaagttatc tatgtactat attaaacaat taagtagtta ttaattgcac ttattagttt
    17221 attaaaaaac tatgtatatt actttgcagt aagactttgg aactaaatct ataatagcaa
    17281 atttaaattt attaaataga ttaccttata acatttcata taaaatatat tgtgaatctg
    17341 agaaaattac tatttttctt tgagtatttt ttataagcag ttctaaaaga ttcaatttca
    17401 tacctattac ttgaagcatt cattaaaata attctaattt aaaaaataat tctatccaaa
    17461 taatagatat ataggactat gttataatat aaaaaaattt atataaaagt taataactat
    17521 acaagattga taaagaaaaa gtcaaaatag ataattgatt agcaaaatag atataattat
    17581 aatgaagtta aatctagcaa tattctccta aaattaaatt tcatttattt aattaaatat
    17641 aatttgacaa tggacaaaaa agtgagtaaa atataaataa aataaaaata acaggttcgg
    17701 ataaagaaaa aggtttctta acctcaagca taaagtatat tatctaagtt atttgagatt
    17761 aagagataaa tccaatctta ggtactgagg aggaaaaaat gcttgatata aaaagaataa
    17821 gagaaaattt agattgtata aaaaaagcaa tggaaagaag gggcgaaaaa gactttaacc
    17881 ttgatgaagt agttaagtta gatgatgaaa gaagaaagat tcttcaagaa gttgaagtta
    17941 tgaaacataa attaaataca gcatctaaaa atataccaaa tcttataaaa gaaggtaaag
    18001 atgtagaaaa tgaaaagatt aaactaaaag agttatctga taaaattaag gtaatagacc
    18061 aaaatcttaa agaagtagaa gataagatgg aatacctatt aatgagaata ccaaatgtac
    18121 cacatccaga agtaccacaa ggtgaaactg atgaagataa tgttgaggta agaacttggg
    18181 gaaagactac tacctttgat tttgaatcta aggctcattg ggaaattgga acagaattag
    18241 gtatacttga ttttgaaaca gcgagtaaaa taactggttc aagattcact ctttacaaag
    18301 gattgggtgc tagattagag agagcattat taaattttta cttagacacg aatacaaaag
    18361 taaatggata tacagaagtc attccaccat ttatggcaaa tagaaatagt tttttaggta
    18421 caggacagtt acctaagttt gaagaagata tgtttaaaat agaaggtctg gactatttta
    18481 tgattccaac atcagaagtt cctttaacta atatacatgc taatgaaata cttaaatttg
    18541 aacaattacc aataaattat acagcttata ctccatgttt taggtcagaa gctggttctg
    18601 ctggaagaga tacaagaggt ttagtaagac agcatcagtt taacaaagta gaaatggtta
    18661 aaatagttgc accagaagaa tcttacaatg agttagaaaa gcttactaac aatgcggaaa
    18721 ctatgctaca attattgaat ctgccttata gagtagtaaa gatatgtaca ggtgatttag
    18781 gatttacggc ttcttttaaa tatgatgtag aagtttggat gccaagttac aataggtatg
    18841 ttgagatttc atcttgttca aactgcgaag atttccaagc tagaagagca ggaataagat
    18901 ttaaaagaga taaagattct aaagctgaat atgtacacac attaaatggc tctgggttag
    18961 ctattggaag aagtgttgca gctatattag aaaattatca acaagaagat ggttctgtgg
    19021 ttgttcctga agttttaaga ccatatatgg gagtttctgt tattaaataa gagttaaatt
    19081 atgtattttt aagcaataca ataatataga agtaataaaa aataattaat ataaaagtga
    19141 tacaataata tagaagtaat aaaaaaatag ttaatataaa agtgatacaa taatatagaa
    19201 gtaataaaaa atagttaata taaaagtgat acaataatat agaagtaata aaaaaatagt
    19261 taatataaaa gtgatacaat aataatagaa gtaataaaaa aaaataatta atataaaaat
    19321 gtgtacaaac taatgaatat tgatatagta agctatcata tgaaagagta tgtacacatt
    19381 ttttttacta atttttcaca atatgaacat aaataattta caggaaagga atcttttatg
    19441 aaaattcaaa atactgagtg gggatatatt gaatggaaac atacttatga tgaaaataat
    19501 ccaaaacaag ctatgaatat atacattgca gtaactatgc ctggtaagaa acattttaat
    19561 catgttcatt atggtcaaga gcagatgata tatattttag agggagaggg tttgtatatt
    19621 ataaatggag tttggaagcc attttatcaa ggtatgattt tttatataga atctggtagc
    19681 acacatgaaa ctataaatac aggcgataga gaaataaaag aacttattgt ttcaaataat
    19741 gtagatgatg taggagagag tgaagttata gatattaatc ctaataatta tttaaagaag
    19801 accttgataa attatagtga aagtacatta aatttgtatg cagcagtaga atcaattcgt
    19861 ggtcaattta tagacccgtt taaaattcca ttaattatat atgatgattc atggaatata
    19921 gttttaaaaa acccttattt tccacttttt tgttttgaaa aatgtaatcc tatgaaattt
    19981 cctcaaaatt gtgattgtat gaatcaaaaa agtagcaatc aatttgtatg tgaatatggg
    20041 ataactatat ataatatacc aatactgtac aaaagcaata gcataggtgt tataagagga
    20101 gggtatgtac tattatctga tttaaattta gatacagagc ataataattt atatgatatt
    20161 ccagagggag ctgctagaag tataaaacgc ctcttgaaac aaatatctaa aaatataata
    20221 aatttttgct cttttaatga tataagaaag gatttacaag aaaaagagaa gactatagca
    20281 agaacatatc attatggaga gcaacttgaa atgaatttaa aagtagctca agatatggtt
    20341 actaatttaa gaataaatca tcacttttta tttaatacat taaattcaat ggcaagcata
    20401 gcactagatg atggtagtta tgatttgtat agtgcaataa tagatttatc aagaatgttt
    20461 agatatacaa tgaggtctga tttaagattt gtagaattag aatctgaaat attatacatt
    20521 aaaaattatt tgaatttaca gaaattacgt tatggagatg cacttaaagt aaaatatctt
    20581 attccagaga agttatataa cctttctgtg ccttttaact ttattcaacc tatcgtagag
    20641 aatgcattta cacatggatt tagagatatt gacacagaaa agcgaattga aataattgca
    20701 agattagatt ctcagtatgc tattatagag attcataata atggaactat cttagatgga
    20761 aataatatag acaaaataaa ggcaggtatt cgtagtaata atggtcatgg tttgtcttta
    20821 atatatacaa aattcacatc tgcttatgga aataattttg atatggatat aaagtcgagt
    20881 gataatgaag gaacttacat aatgattaaa attcctattg aaaattataa ggagtatgta
    20941 tagaacttgt gaaaaattaa taagttttat aaatgttaag aaatatatta gatgttttta
    21001 tgttgtttta tatatcttac ttttttatat tattaagcta aaagggaggt attctcatga
    21061 gaagagcact aataatagat gatgaagaag ctgttctaaa aataataaca cattttatag
    21121 aaatgaaaga tatgccaata aaaattgttg gtaaagcaac atctggtgat gaagcagtag
    21181 ataaaatcat tggtttagag ccagacatag tttttataga tattcaaatg ccaatataca
    21241 atgggttaga agtaatagaa aaaacatctc atttatctaa aaaaataaac tttgtagtaa
    21301 taactgcatt taactatttt gaatatgctc aaaaagcttt gagattaaat gtaaaagata
    21361 tactccttaa accattggat atgaaagaat ttactaagtc tgtagaaaaa attattggat
    21421 atcaatatac caataatgat ttattaaatg agatactgga atatataaat ctaaactata
    21481 gcaatgactt aaagttaaat gattgtgcta gattattttt aaccaatcca tctaatatat
    21541 ctaggatttt taaaagtaat ttaaatacaa cttttatatc atatctaaat catataagaa
    21601 tagaaaaatc tattgaatta cttgaaaata ctacaaaaag tataaatgag attgcagaaa
    21661 tagttggata taatagccta aataactttt ataaaaactt taagatagaa aaaggaatga
    21721 ctccaaaagt ttataaactg aacactaaaa attgaaattg ataaataaaa agtcaatata
    21781 gataattgtt tgagtgtgtc tgaataagta tactttaatt atgataaaaa ttaaagcgct
    21841 ttaaacatat ttaataatat taaaccttat aaaagctgta cctatttata taaaaaaata
    21901 tatataaata aaaagaaaaa gatatacaaa ataaaaaaag tgaattaaat actgtttcaa
    21961 aaagacagaa ttttcattta attcactgag agtttatatt tcttaaagga aggtaacttt
    22021 ataatggagg taatttaata tgtcagacaa agtgaatatg aaaaatatta tctgtattgc
    22081 aggcgcttat ctagcaacag caattggttc tgggtttgct actggtcaag aaattctaca
    22141 attttttgcc tcacaagggt atatgggttt aattggagca ttaatatcaa tggtattgtt
    22201 ttcatttctt ggtgctgaag ttataactag aggtagagaa ttaaaactta aagaaccaac
    22261 aagaatttac actttttatt gtgggaagat tttaggaaaa ttttacgaat ggtttagtcc
    22321 aatattttta tttgggattc ttatagtaat gatttcaggt gcaggctcaa ctttaactga
    22381 atactatggt ataaatcctt acttaggaag agtattgatg gctgttgcta ctttaattac
    22441 agtgtctatg ggtttagaaa aattagtaag catacttgga cgtattggac cagtgatagc
    22501 agtatttaca attgctgttg gattaataag cttgtttaag cattttgatg gtttatctat
    22561 ggttggagaa gctatgaaaa atattgaagt tacaaaagca tcagaaagtc caatcgtttc
    22621 tggtgtaata tataatacat taaatattat tgtaatgatt gtttttttaa caggaatggg
    22681 agcttctatt gaaagtaaaa aggatgcttt ttggggaggt ttattaggtg gaatagtgtt
    22741 tatgactgct ggtatagtta tgtatttggc tatgatttca gatataggaa atttgtatat
    22801 gaaagaaata ccatcacttt atttagcaga taatatatct cctattgtag gtgtatgttt
    22861 ttcagttgca ttaatattag gtatctatac aacagctgtt ccacttttat ggtctgtaac
    22921 caatagaatt gttgaagatg aacatcctaa atttaaattg gtaacaacag taatagctat
    22981 tttggcttgt ataggtggtt ttttaccgtt tggtaaatta gtaaacattt tatatccata
    23041 tacaggttat atggggcttt taattttagt ctgtatggtg tataaagcaa ttacatgtag
    23101 aaaaaaatca acacaaagtc aggaatagag tcacctatta atagttttta tttaaagaaa
    23161 ttttttatat aatccgaatt taaaaagtca aaatagttaa ataaattgca aaacatgata
    23221 atatatttaa ttaaaataat atagtaaaat ataaatataa actaaaatta aatttataaa
    23281 aaatatcttt ataaagatta ttaacttata taatttgtag ttaaattata aaaaataaaa
    23341 aaataaaatt ttggagaggt gttagtatgc atgaacttat tttaatctca gaaaagtata
    23401 aagaagatat tttagatttt tttacagata ttcatatgca ccccgaatta agttttaaag
    23461 aatttaggac tacaaaagct ataaaagatt tattagtaag tttagacata gaaatattag
    23521 atttaggaat ggaaacagga gttgttggat tattaaaagg gaagtatgat ggtcccacag
    23581 tagcactgag aggagatatt gatgcattgc caatatatga agaggttgat attgagtata
    23641 aatccagata tgatggtata atgcatgcat gtgggcatga tatacacaca tcttgccttg
    23701 taggatgtgc atatgtattg tctcatatta gagatagtct tcatggaaat gtaaagttta
    23761 tatttcaacc agccgaagaa gtcaataaag gtgcgaagat gctagtggaa cgaggagtaa
    23821 tggaaaatcc aaaagttgat gctatatttg gacttcataa tcatccagat ataccttgtg
    23881 gaaaaatagg tgttaagtta ggtgggttaa tggcagcagt agacactatt aagatagaag
    23941 taaatggatt tggaggtcac ggaggtatac caaacagaac aatagaccct atagtagcat
    24001 caagtgcaat tataatgggt attcaaacca tagtaagcag aaacataagt ccattagaat
    24061 cagctgttat atcaataggt actataaatg gagggactgc aaataatgta atttcagaaa
    24121 aggtagatat gacaggtaca tgtagaagct ttagtaatga agtacgaaag aaaatatcag
    24181 aaaatttaga aaatatagtt tgtgagatag ctcgtggata tcaagcaact gcaaagctag
    24241 attatttatt tgatttacca gctgttataa atagtaaaga gatgtacaca atagcttgta
    24301 agtctgtgtg tgatttgtat tcagaagatg ctatagttga tccaatacct tctacaggag
    24361 gagaagattt ttctatattt atggaaaagg ccccaggatt tttttactgg ttaggggtag
    24421 gcaataaaga acaagattgt atatatcagt ggcataatcc taaatttaaa gctgataaaa
    24481 attctatatt ggtaggtaca aatgtactat gtcagtctgt tataaattat atggataaat
    24541 taaaaaataa aatttaagat agagcattct ggagataaag tttatgaggg ggtattagtt
    24601 tgaggaaaat taattcacag gaaaaaaaca aagatagtgg attattgatg agggcactaa
    24661 atgttgtaga gagagcagga aataaacttc cagacccagt aaccatattt ttattactat
    24721 gtataattgt tgtaatattg tcagcagtta tatctaattt aggagtagaa gaaatacatc
    24781 cttctacaaa agaagttgta aaggtagtaa atctactaga gaaagagcag attcaatcat
    24841 atttaggtag catagttaca aacttccagt catttgctcc tttaggatta gtactggtta
    24901 caatgcttgg agcaggtgtt gctgagaaat ctggatttat ggaagtgtta atgaaaaagg
    24961 gtataagtaa agttccacaa aaacttgtaa ctgtagcaat cgtatttgca ggtatgttat
    25021 cacatacagc agcagatgta ggatttataa tattaccacc acttgcagca cttgtgtttt
    25081 taggaattgg aagacatcct cttgtaggta tgtttgcagc ttttgcaggt gttgcagggg
    25141 gatttgcagc aaatgttatg cttagtacta cagatgtatt gttagctgga tttacaatac
    25201 cagcagctca aatgatggac ccatcttacc aaggcaatcc agcaatgaat ctttattttg
    25261 cttttatatc agcgatagtt ttaacttttg caggagcatt tgtaactgaa aaatttatag
    25321 ctcctcgttt tacaaaatat aatgattcaa caaatgataa ggatatacag gaattgagtg
    25381 aacttgaaaa taagggtata aaatacgctt tattatcatt gttagttgta gtagtagtga
    25441 tagttgcctt atgtataggt gacaatgcat ttatgaaaga tccagagact ggttctatac
    25501 tatctagtaa tgcaccttta atgaaaggta tagttcctat aatcactatt atatttttaa
    25561 ctccaggatt agtgtatgga aaagtaagta agaagataaa aagtgacaaa gatttagtat
    25621 ctatgatggg tagtagtatg agtgatatgg gtggatatat agtcctagca tttatagcat
    25681 cccagtttat taatttattt aatcttagta acttaggaac aattttatca ataacaggag
    25741 caaaattact agcagagtcg gggataccta gctatggatt gataatagga tttatattgc
    25801 tatctggatt tatcaattta tttgtaggaa gtgcttctgc aaaatgggcg attttagcac
    25861 ctatatttgt tcccatgttt atgctactag actttaatcc agcattaact caaatagctt
    25921 atcgtattgg agatgcttca acaaatccaa taagtccact ttttccgtat tttccagtta
    25981 tattggcctt tgccagaaga tatgataaag atataggtat aggtactgtc atatcgaata
    26041 tgattccata ctcagtggta tttacattaa tagaaataat aatattgtta ctattcatgg
    26101 gtattggcat accattagga cctggtggag gaatatctta cgttttatag accattataa
    26161 taaatttatc aatgaagatt aaactaataa aatatattct ttaattaaga ggaggattat
    26221 tatgttaaaa acaacaaaaa tttcagaccc agagttatac aagattgtag ctgatgagtt
    26281 ggttagacaa gaacacaata ttgagatgat agcttctgaa agtacagcac caacagaagt
    26341 acttgaatta agtggatgtg tatttacaaa taaaacagaa gaaggctatc caggggctcg
    26401 ttttcaagca ggttctgaag aagcagataa acttgaaacc ctagcaataa aacgtgcaaa
    26461 agaagtattt ggagcagaac atgtaaacgt acaaccgtat tcaggttcta cagctaacta
    26521 ttgtgtatat tcatcaatat taaaaccaaa tgacactgtt ttaagtatgc gtcttgacca
    26581 aggtggacac ttgacacatg gtagcgcagt taatttttta catgacattt ataaatatga
    26641 attttatggg gtagacccaa atactggacg tatagattat gatgctcttg aagcaaaggc
    26701 aaaagaatgt agaccaaaac ttataattgc aggggcaagt tcatatccaa gattaataga
    26761 ttatgaaaga atatcaaagg ttgcaaaaga agtaggagct tactttatgg tagatatggc
    26821 acatgttgca ggattagtcg cagcgaaagt aatacctagt cctgtacctt atgcagattt
    26881 tgtttcttca tctacgacaa agactttctg tggaccaaga agtggtattg tactttgtaa
    26941 agcagaacat gcaaagaaac tcgataaagg tgtttttcca ggaacattag ggtctataca
    27001 tcttaataca gttgcagcaa aagccttttc actattatat ttaagtacag ataaatttaa
    27061 aaagataatg gagcaagttg tagtaaatgc tcagactcta gcttctgaac tgatttcaca
    27121 tggttttagt attgtaagtg gaggtactga taatcatata gtaatggttg acttaagaag
    27181 taagaatctt acaggaaaac aatttgaaaa agctcttgaa tatgtaggaa taacagttaa
    27241 taaaaatgtt atacctgacg acccacaatc accatttgta actagtggtg ttcgtatagg
    27301 tcttacatca atatctcaaa gaggtctgaa agaaaaagaa gttatacaga tagcaggaat
    27361 aatgaataag gtagcagaaa atattgataa taaagaagta ttagatgaat gtaaagcaga
    27421 ggcacaagaa cttatatcta agttcccatt atatccagaa gggtattttg aagattaaat
    27481 attttgattt gtatagctca caatttataa agcctgtaac ataaaattta ataatatata
    27541 accactataa ggtgttgatt aatcaacacc ttattttttt tgaaaagtaa tatacaagct
    27601 atcataagca tataattcct tatagataat taacaagggg gataaagtta tgaggtatta
    27661 taataaacct accaaagaag tattaaaata cttaaaaaca aatccagaaa ttggacttga
    27721 tgataatgaa gtagaagaaa ggaagcttag atatggatta aatgaattta ctataaaaga
    27781 aggtagaaca ttttgggacg agttgggaga aagtttgaca gaacctatga tattaattct
    27841 cataggagca gcagtaataa gttcttttgt gggagaatta catgatgcat taggtatttt
    27901 aggggctatt tttattggaa tttctatagg cataataact gaaggaaaat ctaaaaaagc
    27961 agctcaggcc ctgtcaaaat taacagaaaa tatagaagta aaagtactta gaaatggaaa
    28021 gataattaaa atatcaaaaa atgatttagt accaggagat attgtatata tagaaactgg
    28081 agatatgata ccagcagatg gaagacttat acagtctata aatttgaaac ttagagaaga
    28141 tatgctaaca ggagaatctg atgatgtagc taagaatgct gatgctgtat tagatatgga
    28201 agtagtttat tctaaaacag aaataatcga acaggatgca atacctgcaa agcaagtcaa
    28261 tatggtattt ggagggactc ttgtagcata tggtaggggc ataatggttg tcactcatac
    28321 tggtgataag acagagatgg gtaagatagc acagaattta tcaaatgaag accagcaaac
    28381 tccacttcaa attaaattag ggaagttggg tgctaaaata gcaggaatat caggcgtaat
    28441 agctacttta ttgtgtatgt ttatgattat acagatgcag agaaaaggta tgttaatttt
    28501 agatacaagc agtatattat ctttcttaca atcattagaa cctgctaaaa atgcatatat
    28561 ggtttgtatt gcattaatag ttgcaactgt tccagaaggt ttgccaacta tgataaatat
    28621 taccttagct ataacaatgc aaaagatggc aaagataaat gcattagtaa caaagaaaga
    28681 agcctgtgaa actataggtt ctgtttctgt aatatgttcc gacaaaacag ggactttaac
    28741 acaaaacaaa atgatggtag aggtagcata tgttgatggt aaatatataa gtggaggaga
    28801 gtatcaaagt aacagctatt ttgaacaaaa ctgtatagta aattcaactg cagatataga
    28861 aaaagaagat aattcattta aatatatagg aagtgcaaca gagtgtgcat tacttttata
    28921 tcataatgat aagaactata atgaaatgag aaaacaaacg tatttgatat cacaaatacc
    28981 atttagttca gaagaaaaga agatgtcaac attaattcgt caagaggatt cagatatatt
    29041 actttcaaaa ggtgctccag aagtattatt aaaaaaatgt tcttatgtac aacaaggaaa
    29101 aaatattgtt ccaataactc caaaagtaga aaaaagtatt ttagatgaga ttaagaagtt
    29161 acaaattaaa tctatgagaa cattaggatt tgcttataaa aaaatgagta attctaaaac
    29221 agaagttgca atgacttcag aaggtgaatt gaatttaata ggaaattcaa gaagttatat
    29281 gaaggaagat aatttagttt ttagtggatt tgtaggaata gtggacccat tgagagaagg
    29341 ggtcaaagat tccattgata aagcatttaa tgcaggagta gatgtaaaaa tgcttacagg
    29401 agataatata aatacagcaa cggctattgg aaatgaatta ggattattaa atgatgggaa
    29461 aaaggcagtt gaagcaacat atatagatgt attgacagat aaagaactga gagaagaaat
    29521 taaaggaata tctattgttg caagaagtaa accagataca aaaatgagaa tagtttcagc
    29581 gcttcaaaaa agtggcgaag ttgtagctgt aacaggtgat ggtataaatg atgccccagc
    29641 tcttagtcaa gcagatgttg gaattgcaat gggtatttca ggaacagaag tatctaaaaa
    29701 tgcagcagat ataatattga ctgatgatag ctttagtact atagtagagg gaataaaatg
    29761 gggaagaggt atatatgaga actttcaaag atttatacag tttcagctta ctgtaaatat
    29821 agtagcattt ataattgcta taatatctca acttactggt aaggatatgc catttactac
    29881 aatacaatta ttatgggtaa atataataat ggatggtcct ccagccctag cattaggatt
    29941 agaacctgtt agagattatg tattgaaaag aaaaccaata aatagacatt ctggaataat
    30001 agctaggtct atgtttgtaa atattattat aaatgctatt cttataatta caattgtatt
    30061 tactcaatca gcttttaata tattgggagc tacatcagaa gaacaaggta ctgttatatt
    30121 ttctttattt gcgttcagtg ccctttttaa tgcattaaat tgtagagaat ttggtttaaa
    30181 tagtacaata ccaaatttct ttaaaaataa actggcattg caaataatag tagttacagg
    30241 gataattcaa ataatattta cacaagtgtt ccagagtttc tttaattctg tttctttgga
    30301 ctttgatatg tggataaaaa taatactatt tgcatccact atattattgt caaatgaatt
    30361 tgtaaagcta atacttagaa caatgaaaaa tagtagaagt atgaactcta atagtaaaaa
    30421 ttaattctta tgaaaccgca aagtactata tattgtaact ttgcggtttt tgtttgttta
    30481 atgttgtttt atattaccat tttcatattt aattttttat taatctgaac acaatcttcc
    30541 attgtaattg cttcatctgt gtcaacacgt tcaaagtcaa tatctaactt ttgtaatatt
    30601 ttgtatacct ctttttgcaa tggagtatca aatttaatag gtgattttgt gtaaatatca
    30661 ctgataaaaa acatgattta tttcattcct ttccgtttaa aatcttatag ttatagtgta
    30721 tcataaggtt ataaaaacat aaatatagta taaatttaaa atatgtaaag acaaaacaaa
    30781 aaaatcatat tctaagtaat acttgggaaa aatgatatta tcataataaa taatctttgt
    30841 aaaaacttat atagaatcta ttaaacagac actaagccat aaaaacttta cgaaatataa
    30901 aatttgtatc taaatagata tatagaataa tattttactg aagtagagtg atttttaaga
    30961 attttatata taaattatgt tatcataaat aaagatatag attataaaat ttgttaataa
    31021 aataaagggc agggaggtaa gtttcatgtt ttccaaaatc ctagtcaaaa catttatcag
    31081 agatagcgaa aacgttcaaa atacagatgt tagaaataag tatggttatg tagctggtgt
    31141 agtaggtata ttgtccaatt tacttttatt tgtgataaag gtattcatag gaatgttcac
    31201 atctagtata gctataatgg ctgatgcatt taataatctt tctgatatgg catcttcagc
    31261 aataactatg ataggtttta aattagcaag taagccagct gacaaagaac atccttttgg
    31321 tcatggaaga atagagtatt tatcagcatt aatagtagca tttatggtga tgttggttgg
    31381 acttcaattt gtaaagtcat ctatagaaag gatagtaaat ccaattcctg taaaatttga
    31441 agtaatacca cttatacttt taattgtatc tataatgatt aaaatctggt tgagtcgttt
    31501 taataaattt gtgggaaata aaattgattc ttcagcctta aaagcagttt cacttgatgc
    31561 tttaggagat gtatttactt caagttgtgt agttatatca tttattgtag ctagatttac
    31621 taattttcct atagatggat atgttggtat cgtagtatca ttagtgatac tttatgctgg
    31681 tttctctctt gtaaaagata ctataaatcc tcttttagga gaagcaccgg atgaagaaat
    31741 ggtaaattct attatagagc tattgttatc ttataaatat ataataggta cacatgattt
    31801 aattattcat aactatggag ttggtagatg tattgcatct atacatgcag aaattccatc
    31861 caacatagat ataatggaaa tacatgaaat tatagatact gcagagagag aaatatctga
    31921 aaaattagat atatatctag taattcacat ggacccaata tgtcttgaag ataaagaagt
    31981 tatgtcagcc aaaaaagaac ttgaagaaat acttaagaaa aatagtcttg taaaatctat
    32041 gcatgatttt agaatagttg gaaaaggaag caagaagaat ttaatatttg atatagtagt
    32101 taatccttcc gaattttcca aagacatgtc agaggatgac ttaaaagaag atataacaaa
    32161 attagttaag gagataaatc ctgaatataa ttgtgtaatt gttgtagata aggattttat
    32221 ttaattatta attaatctca taaaaggtaa aaaatgtaaa aaagatagac aatagtatag
    32281 aggcataata taatagaagt atataattgt atttttaata taaataaagc aattttaatt
    32341 gtttttatga aatattatag agatgttgaa gtatattcta ttattgggca ccttatggat
    32401 atactgagtc agtggtgcaa ccggctatga atataaattt atttattttc atagcttttt
    32461 ttatataatt ttttagtaaa aattaattta tatacgatat taatggaaga ttataaacta
    32521 tagataacct agagggggaa aatttatatg aagaaaggaa ataaaaaggc attattaatt
    32581 agtttaatta tgattttaag tatggtggtg tctacaatat atccaactgt atcttatgct
    32641 tcagaattag gagagaatag tcagattcaa agtggttcaa ctaattcatc aactggagag
    32701 gagaaggaaa gcgacaataa aaaaccagaa cagactccag agaaagataa ggcaacagat
    32761 aataaaaaac cagaacagac cccagaggaa gataattcaa cagataataa aaaaccagaa
    32821 cagacttcag aagaagataa gtcaacagat aataaaaagc cagagcaaac tccagaagga
    32881 gaaaagccaa tagataataa aaagccagag caaactccag aaggagaaaa gtcaatagat
    32941 aataaaaagc cagagcaaat accagaagaa gataagtcaa cagataataa aaagtcagaa
    33001 caagctttag aagatgaaaa accactagac aataaaaata cagaaaaaac tccagaggaa
    33061 gataatctat tagaagatga aaatttattg aaagttttag aagaagaact taatgaagac
    33121 tatggatttg ttgtaaaaat taataacaat acaatagaaa cggaatcaat gaaaaaaata
    33181 tcatttaatt taacttatac tccaacatca aaaggaatac aagcaggaga ttcaataact
    33241 tttaaggtgc cagatgtatt caataaagtt aatttagatt atacaagtga atgctttgat
    33301 aaaacagaaa gtaatggaga gtatacactt acatttagag aattacctaa tggccaaagt
    33361 gtaatgcaag gtaaaatagg tttggaagca tatgttaaaa aggttgatga agatactaat
    33421 gcaaaaatac atattgaaac cactggaaaa atagaaagtg gaagtggaga tatagatgta
    33481 gaaataaaac ctggagataa aacggatgtt ccagatgcta aaggaacact taaaaagctt
    33541 gttgaaggaa gaaaatcaac tactgtattt atgccagtta aaaataaaga tataaattac
    33601 agtatacaag taaatgaaaa gcaggaagag ttaaaagata ttattttgta tgatgagctt
    33661 cctgaaggat tgacattgat aaatggttct gtaagtgtag taacaagtga tggaaaagaa
    33721 gttagtgatt ttaatattga acagagtaaa aattctattt cagttaattt tggaaatata
    33781 gataaatcgt atactgttaa gtataaggct agaatcagtg acaaaaatgc aaaacatggt
    33841 aataaatata agaatgtagc taggattgaa tctgatggta agaagataca agaagatgat
    33901 gctactgtaa gtatatttga tagaggagat aattatcttc ttactaaagg acattcagga
    33961 gcaacaaata ttactcaagt tggtcaagta ataaactatc agatttcaat aaatgatgat
    34021 aaatcaccaa taagtaatgt tgttataaca gataatatac cagaaggtat gagattgact
    34081 acttcaggag aggatgggca tgattttaga gttgtagaag ttccaatgaa tgggagctgg
    34141 actccttgga gtaaagaaaa aattgccaat aatatttcat ataaagtaga agaaaaaaga
    34201 aatgaaagtg ggcaagtaga taaagttata actggattta caattaatct cagtaaagaa
    34261 gaagtagaat caaagttttt tatagcttat acattaaagg taattagtat tgaagatagt
    34321 tatataaata gagctgtcct tgatgcaaat aatagtgaaa ttgataaaaa tgatgagata
    34381 aactttaaga aaaattctgg attaataagc gcaaaaaaag aagttgataa aaaggtttta
    34441 aatagtagtg ataaccagat tgttaaatac aaaatcaata tgtcaactta tggggtttat
    34501 gatgcaggac aagtaaatct tttggatgaa gtaaacccat ctctaaaaat atctaatata
    34561 aaatattctg ataatcttga acttaaaaaa gaagctggag atggaaaaaa tacaataaga
    34621 ttagtaaata aatacgaatt taaacagata aaagaagggg aacctgtgca aagctgggtt
    34681 acatttgatg cagacttcac aaatgtaaaa gttggggaga ctatacaaaa tgtggctcag
    34741 attaatggtt caagtcctcc gggagtagag actactaagc aaggttatgc ttttgaagct
    34801 aaaaaagtgg atgctttaga taaaaatgta ctttcaggtg ctaagttcaa tttagaagat
    34861 gcatttggaa atatagtagt taaggattta gttagtgatg aagatggtgt aattcaatct
    34921 agtgtaaaaa aaccaggaat atattattta gtagaagtga tggctccaag tggatatgaa
    34981 aaactgaagg ataaaattaa ggttgaaatt ggtaataaag atattggtag aatagtagat
    35041 atcggaaata tagaaaacat aagacaagaa aatccaccag taaatccacc tgtaccgcca
    35101 aatacagatg aaccaatagt aaatccacca gtgccaccaa gcacagataa accaaggaaa
    35161 ccatcaaatc catctgatac agaggatact atagttataa atccaccagt accaccgtca
    35221 gaagatataa taaatcctcc aataccagag atattgaatc caccagtacc accatcagaa
    35281 gaggtgatag aaactcctgt taaacagatt gttccaatac cagaagtggt aaagccatca
    35341 gtgtcagaag aaaaaaataa taaagctaaa gatgatactt tggtaaatcc accagtacca
    35401 ccaaaaacag gggatagtac tacaattatt ggagaaatac ttttagttat aggagctatt
    35461 gtaggacttg tcgtattaag aagaaataaa aatacaaatt agaccctaac attataaaaa
    35521 atataagtta tattaaaaaa tatctt
//
LOCUS       DAGQWR010000022        35350 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.022, whole genome shotgun sequence.
ACCESSION   DAGQWR010000022 DAGQWR010000000
VERSION     DAGQWR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.022"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..851
                     /locus_tag="MIW95_000957"
     CDS             <1..851
                     /locus_tag="MIW95_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBY3150304.1"
                     /translation="VYEVKEPKENAVGNNITQIDISTDNNLMLYVAYLWLVVTIVIAV
                     YTFIKYTRFKNLVVDVSYDIEDNDVNCIYKNLLKELNIKKKIELRGSDELISPAGMGL
                     FKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYVMSNK
                     VNLDCELCCDESVLTDCSLKDKKEYALALIKSIKLSKNYNSGILTTEFNKTSLEKRLD
                     SIVKKKGKNGILVLLILITLFSVTYFNFESISFDNSNSLRNMGILQKSGVDLKEEDDI
                     SMRVIN"
     gene            917..1504
                     /locus_tag="MIW95_000958"
     CDS             917..1504
                     /locus_tag="MIW95_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBY3150305.1"
                     /translation="MKNLKKILIITGCLTLISTSLVFANTTTDVDTSKDDDSIISSCT
                     ITGYEFTYENAPQSVKFDYEKKCEALNIKPSKNDIIFVNKDSIDANDVENYVEPKAYI
                     IRYKKGYVFEVTGPKSYTLNVSNTVVGYNRVESGNPVHLVQLLCRTLDGSSIDDDSEF
                     GPATYNAVKSLQGRLGLTRDGIVGRSTWDAAGRRL"
     gene            complement(1882..2643)
                     /locus_tag="MIW95_000959"
                     /pseudo
     CDS             complement(1882..2643)
                     /locus_tag="MIW95_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612258.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3251..3628
                     /locus_tag="MIW95_000960"
     CDS             3251..3628
                     /locus_tag="MIW95_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY3150306.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDATVTSKDVIEVMEQKYGWKQTTT
                     LTLLSRLVKRGFLNAQKIDRYTHYTVIVKHKDYLSFETKDFLSNIHDDSLKSLISALH
                     DDENIGKDTLDFLESYFDNLKNE"
     gene            3621..4655
                     /locus_tag="MIW95_000961"
     CDS             3621..4655
                     /locus_tag="MIW95_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBY3150307.1"
                     /translation="MNNYMLYVFDNLIKTTIVCSFGICLLLFLKRYFFKRFSKRFNYY
                     IWLVIVFRMLLFLFNYTVMYEVKEPKENTVGNNITQIDVSIDNDFMLYLVYLWLFVTI
                     AIAVYTFMEYTKFKNLVVDVSYDIEDHDVNCIYKNLLKELDIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYTYSKDELTWILKHELMHFKNKDILIKFLVLSVKIMYWFNPLVYI
                     MSNKVNLDCELCCDESVLTDCSLKDKKAYALALIKSIKLSKNYNSGILTTEFNKTNLE
                     KRLDSIIKKKGKSGILMLLILFTLFSVTYFNFESISFDNSNYLRNIGILEKTGADLKE
                     EDDISMRVVN"
     gene            5134..5307
                     /locus_tag="MIW95_000962"
     CDS             5134..5307
                     /locus_tag="MIW95_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBY3150308.1"
                     /translation="MHLVQLLCQQINGSDIDPDSKFGPATYNQVKILQGRLGVTKDGV
                     VGKATWQAVGERY"
     gene            complement(5392..6414)
                     /locus_tag="MIW95_000963"
     CDS             complement(5392..6414)
                     /locus_tag="MIW95_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBY3150309.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTLLNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNSGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTKLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(6745..6927)
                     /locus_tag="MIW95_000964"
     CDS             complement(6745..6927)
                     /locus_tag="MIW95_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150310.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMVKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            7240..7659
                     /locus_tag="MIW95_000965"
     CDS             7240..7659
                     /locus_tag="MIW95_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBY3150311.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            7790..9631
                     /locus_tag="MIW95_000966"
     CDS             7790..9631
                     /locus_tag="MIW95_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY3150312.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFVENKIGVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSIEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNQSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYTIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKN
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIETEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMFMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIREIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            9658..11544
                     /locus_tag="MIW95_000967"
     CDS             9658..11544
                     /locus_tag="MIW95_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY3150313.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKEDSDIISKIYIYLT
                     LSAISIRKYDKANNFYFSAKKVIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSKNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELRKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            11552..13552
                     /locus_tag="MIW95_000968"
     CDS             11552..13552
                     /locus_tag="MIW95_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3150314.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTREFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            13814..15073
                     /locus_tag="MIW95_000969"
     CDS             13814..15073
                     /locus_tag="MIW95_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY3150315.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVEEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            15134..16921
                     /gene="iorA"
                     /locus_tag="MIW95_000970"
     CDS             15134..16921
                     /gene="iorA"
                     /locus_tag="MIW95_000970"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBY3150316.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEMNDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            16923..17498
                     /locus_tag="MIW95_000971"
     CDS             16923..17498
                     /locus_tag="MIW95_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBY3150317.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            17551..18621
                     /gene="buk"
                     /locus_tag="MIW95_000972"
     CDS             17551..18621
                     /gene="buk"
                     /locus_tag="MIW95_000972"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBY3150318.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            18839..19540
                     /locus_tag="MIW95_000973"
     CDS             18839..19540
                     /locus_tag="MIW95_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150319.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKRSN
                     EHKCKSSIVIKNEQN"
     gene            19599..20105
                     /locus_tag="MIW95_000974"
     CDS             19599..20105
                     /locus_tag="MIW95_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBY3150320.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEMYELLYE
                     IDNNKLNI"
     gene            20280..21302
                     /locus_tag="MIW95_000975"
     CDS             20280..21302
                     /locus_tag="MIW95_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBY3150321.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(21449..21757)
                     /locus_tag="MIW95_000976"
     CDS             complement(21449..21757)
                     /locus_tag="MIW95_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBY3150322.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(21817..22245)
                     /locus_tag="MIW95_000977"
     CDS             complement(21817..22245)
                     /locus_tag="MIW95_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150323.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(22468..23925)
                     /locus_tag="MIW95_000978"
     CDS             complement(22468..23925)
                     /locus_tag="MIW95_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBY3150324.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            24343..25257
                     /gene="nadA"
                     /locus_tag="MIW95_000979"
     CDS             24343..25257
                     /gene="nadA"
                     /locus_tag="MIW95_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBY3150325.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            25270..26571
                     /locus_tag="MIW95_000980"
     CDS             25270..26571
                     /locus_tag="MIW95_000980"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBY3150326.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            26552..27388
                     /gene="nadC"
                     /locus_tag="MIW95_000981"
     CDS             26552..27388
                     /gene="nadC"
                     /locus_tag="MIW95_000981"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBY3150327.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            27644..27976
                     /locus_tag="MIW95_000982"
     CDS             27644..27976
                     /locus_tag="MIW95_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBY3150328.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            28138..28323
                     /locus_tag="MIW95_000983"
     CDS             28138..28323
                     /locus_tag="MIW95_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150329.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            28350..29435
                     /locus_tag="MIW95_000984"
     CDS             28350..29435
                     /locus_tag="MIW95_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBY3150330.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLYNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSTIPKMKEVIGNHA"
     gene            29428..29886
                     /locus_tag="MIW95_000985"
     CDS             29428..29886
                     /locus_tag="MIW95_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150331.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            29921..31012
                     /locus_tag="MIW95_000986"
     CDS             29921..31012
                     /locus_tag="MIW95_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY3150332.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKAGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            31017..31241
                     /locus_tag="MIW95_000987"
     CDS             31017..31241
                     /locus_tag="MIW95_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150333.1"
                     /translation="MENIKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            31251..31814
                     /locus_tag="MIW95_000988"
     CDS             31251..31814
                     /locus_tag="MIW95_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150334.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            31775..32404
                     /locus_tag="MIW95_000989"
     CDS             31775..32404
                     /locus_tag="MIW95_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3150335.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            32444..32581
                     /locus_tag="MIW95_000990"
     CDS             32444..32581
                     /locus_tag="MIW95_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY3150336.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            32581..33333
                     /locus_tag="MIW95_000991"
     CDS             32581..33333
                     /locus_tag="MIW95_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3150337.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            33346..33831
                     /locus_tag="MIW95_000992"
     CDS             33346..33831
                     /locus_tag="MIW95_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150338.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYENIMVVDGNSINFIYPVSALQTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            33838..33987
                     /locus_tag="MIW95_000993"
     CDS             33838..33987
                     /locus_tag="MIW95_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150339.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(34123..34944)
                     /gene="yidA"
                     /locus_tag="MIW95_000994"
     CDS             complement(34123..34944)
                     /gene="yidA"
                     /locus_tag="MIW95_000994"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBY3150340.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNEAPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
BASE COUNT        13794 a         3782 c         5958 g        11816 t
ORIGIN      
        1 tagtgtatga agtaaaagaa cccaaagaaa atgcagtagg aaataatatt acacaaattg
       61 atatttctac agataataat cttatgttgt atgtagctta tttatggtta gttgtaacta
      121 tagtcattgc agtttataca tttataaagt atacaagatt taaaaatcta gttgttgatg
      181 tatcttatga tattgaagat aatgatgtaa actgtatcta caaaaacttg ttgaaagaat
      241 taaatataaa aaagaaaatt gaattaagag gttctgatga attgataagc ccagcaggca
      301 tgggattatt taaatcttac atattcttac cagactatcc ttatagtaaa gatgaactaa
      361 cttggatttt gaaacatgaa ttaatgcatt ttaaaaataa agatatttta ataaagtttt
      421 tagtgttatc tgttagaata atatattggt ttaatccact tgtttatgta atgagcaata
      481 aagtaaatct agattgtgaa ttatgttgtg atgaaagtgt tttgactgat tgttctttaa
      541 aggataagaa ggagtatgca ttagcactta taaagtcaat taaacttagt aagaattata
      601 atagtggtat tttaacaaca gagtttaata agacaagtct ggaaaagaga cttgacagta
      661 ttgttaagaa aaaaggtaaa aatggaatat tggtgttatt gattttgatt acattatttt
      721 cagttacata ttttaatttt gaaagtatta gttttgataa ttcaaattct ttaaggaata
      781 tggggatttt acaaaaatca ggagtagatc ttaaggaaga agatgatatt agcatgagag
      841 ttataaatta atagattaat gtatgtaatg aaaaggtata ttgtcgtatt attaataaat
      901 taggaggaat tatgaaatga aaaatttaaa aaaaatttta ataattacag gttgtctaac
      961 tctaatatct actagtttgg tatttgcaaa tactactact gatgtagata catcaaagga
     1021 tgatgattca ataataagtt catgtactat aactggttat gaatttacat atgaaaacgc
     1081 accacaatct gtaaaatttg attatgaaaa aaaatgtgaa gcgctaaata taaaaccatc
     1141 taaaaatgat ataatttttg ttaacaaaga ttctatagat gcaaatgatg tagaaaacta
     1201 tgttgagcca aaagcatata ttattagata taaaaaaggt tatgtttttg aagttactgg
     1261 acctaagagt tatacattaa atgtaagtaa tactgtagtc ggatataatc gtgttgaatc
     1321 aggtaatcct gtgcatttag ttcaactttt atgtagaacc ttagatggtt caagtattga
     1381 tgatgatagt gaatttggac ctgctacata taatgcagtt aagtcacttc aaggaagact
     1441 tggattaaca agagatggaa ttgttggaag aagtacttgg gatgcagctg gaagacgtct
     1501 ttaaaaagaa agctattgca cagataattt taaattgctg aatctgtaaa catttacatg
     1561 tattctttaa ataaaaattt attttttaag tgataattaa atttttacta atagggctat
     1621 ggctttaaaa atcatagcct tattttgata tcttttccat ttttgcaaaa gctattaacc
     1681 ctttaagaaa atttcttgtt tttttgaaaa atttctttct tcaaaataaa tacttaaatg
     1741 agctaaaatt tgagcacaat ttctaatttt ctgagtccat ttttaagaaa tatatatggt
     1801 tgttaagtat agtgatttgt gcaaataatt atccaaagta aaaatagatt tcaatttagt
     1861 aacttttctt aataatctat tctaacttcc cattgaattt gtagtatata tcgatgtatt
     1921 tatttcttga gagtacttaa aatatgttac taattcattc caattgtttc accaacctct
     1981 taatactatt gtatattgat tgtactattt tttctctagt gatagctcac ttaatgctac
     2041 ctcttctgta attgtagcat atctttcttt aaatctttta cagattcttt tctattttta
     2101 tatgatacat aatttaaagt atttttaatt tgatgcacaa catatctttg tacttaagta
     2161 tcaggaaata tcgctttaat tgcattagaa aattcaacta gtctatctat agaagctgct
     2221 attaatacat ctttaattcc catgtttcta gcatctataa gcacttttag ccaatacttt
     2281 gaaatttcat tttctccaat ccatattctt aaaacctctt ttatgctaca tatagctata
     2341 tagactgttt tgtttattat tcgacctcac ttctaacttt ataatgtata gcatctataa
     2401 atacaattga ataaattata ttaagaggtc taagttacca tttatgagtt aaaggaataa
     2461 ctttatcagt agttttagtt attagtgaat gagataatat caattccata agtactttta
     2521 agatgactta atatattttg agtagacatg ccttttgagt atattccaat cacttgattt
     2581 tttattccta atatatcatt ttctatcata acctaaaagt tcattaagct ccgcttctaa
     2641 catttaatgg attgtacttt caaataaatc ttttagtata tcttgaatat ctgtaattgt
     2701 tttggcatca tattcttcaa taaatttttt taatatttta gtttttgtta aacataaaaa
     2761 gtgaacatct tcctttgtat agtagtgata ttttaattat cacaactttt tagaagaata
     2821 caaactttat tttaaagaca ctgaaaatgg tgtttttata cctttatttg ctatgtataa
     2881 ttttacttat aacataaact tatataaaac agatatttta ataacacaaa ggtacaatta
     2941 ggagattgtt aaaatttatt agatattact ctagtttatt taaaaaaaag tattataata
     3001 ttccttgtgt gaaaatatag gaatttttat taaataattt taatgtggaa ataacattct
     3061 gcactattat aattaaatat gtaaaaagca aaatacttga acaactatat tttaaaatct
     3121 aacttttagt attttaaaaa attagaaaat taataaaaag tacaaaaatc cacatttttt
     3181 cataaagcgt ttgactaaaa aattcacata ttgtagactt aaagtagcaa gagttttgag
     3241 ggaggtgtaa atgatgataa gaaaaatacc acaagcagaa ctaaaagtaa tgaaatttat
     3301 ttggaaagta gatgctacag tgacatcaaa agatgttatt gaagttatgg aacaaaaata
     3361 tggttggaaa caaaccacta cactaacact tttatcgagg ttagtaaaaa gaggattctt
     3421 aaatgctcaa aaaatagaca gatatacaca ttacacagta atagtaaaac acaaagatta
     3481 tttaagtttt gagacaaagg atttcctaag taatatacat gatgattctt tgaaaagctt
     3541 aatttcagca ttacatgatg atgaaaatat aggtaaagac acgctagatt ttttagaaag
     3601 ttattttgat aatttaaaga atgaataatt atatgctata tgtttttgac aatcttataa
     3661 agacaactat tgtatgtagt tttggtattt gtctattatt atttttgaaa agatattttt
     3721 ttaagaggtt tagtaagaga tttaattatt atatttggtt ggtaatagtc tttagaatgt
     3781 tactttttct atttaattat acagtaatgt atgaagtaaa agagcccaag gaaaatacag
     3841 taggaaataa tattacacaa attgatgttt ctatagataa tgattttatg ctgtatttag
     3901 tttatttatg gttatttgta actatagcca ttgcagttta tacatttatg gaatacacaa
     3961 aatttaaaaa tctcgttgtt gatgtatctt atgatattga agatcatgat gtaaattgta
     4021 tctataaaaa tttgttaaaa gaattagaca taaaaaagaa aattgagtta agaggttctg
     4081 atgaattgat aagtccagca ggtatgggat tatttaaatc ttacatattc ttaccagact
     4141 atacttatag taaagatgag ttaacttgga ttttgaaaca tgaattaatg cattttaaaa
     4201 acaaagatat tttaataaag tttttagttt tgtctgttaa aataatgtat tggtttaatc
     4261 cacttgttta tataatgagc aataaagtaa atctagattg tgaattatgt tgtgatgaaa
     4321 gtgttttgac tgattgctct ttaaaagata agaaagcata tgcgttagca cttataaagt
     4381 caattaaact tagtaagaat tataatagtg gtattttaac aactgaattt aataagacaa
     4441 atttggaaaa gagacttgat agtattatta agaaaaaagg gaaaagtgga atattgatgt
     4501 tattgatttt gtttacatta ttttcagtta catattttaa ttttgaaagt attagttttg
     4561 ataattcaaa ttatttaaga aatataggga ttttagaaaa aacaggcgca gatcttaagg
     4621 aagaagatga tattagcatg agagttgtaa attaatagat ttttatagta ttataattta
     4681 ttaacaaatt aggagaagtt ttgaaattaa aaatctaaaa aaaatcttag tagtcacagg
     4741 gagggtgact ctaatatcta ttagtttagt atttgcaaat actaataatg atgtaaatgt
     4801 atgaaaaagt aatgaagagc aaacaacaaa ttcatttaca ataactggtt acgaatttat
     4861 ttatgaaaat gtaccacaac ctataaaatc tgattatgaa aaaaatgtaa agaattaaat
     4921 ataaatccat ctaaagatga tacaatcttt attaatgaaa aatttataga ttcatataat
     4981 attaatatag atgattataa tattgaacca aaagcattta ttattagata taaaaaagat
     5041 ttaaatcgtt ttgatgctac tggtcctata agttatagcg taaatataaa taccactgta
     5101 gtaggatatg gacatgtaga aaaagggaat cctgtgcatt tagtacaact tttatgtcaa
     5161 caaataaatg gttctgatat tgatccagat agtaaatttg gacctgctac atataatcaa
     5221 gtaaaaatac ttcaaggaag acttggtgta acaaaagatg gtgttgttgg aaaagccact
     5281 tggcaagctg tcggagagcg ttattagaaa agaggttgtc gcactaagaa atagggcgac
     5341 aactttttta tatagaatat attaatcagt atcttagagt ctagaaatat tttacaataa
     5401 attccatata actctaccat ctatatcctt aacattttga caaccagtca aaatcatagt
     5461 ctcacataac tcattcctaa ccttctctat aaaaaactcc acaccatcaa gaccaccgcc
     5521 aaaagaagca gtaacaaaag gtcttcccat caaaacagca tcagcaccca atccaagcat
     5581 cttaactaca tcaacaccag tcctaactcc accatcaaca agtattgtta tctttccctt
     5641 tacagactta gctatatctg gaagtacctc acaagtacca ggagtataat caagtactct
     5701 tccaccatga tttgaaacaa taatggcact agcaccagac tctacagcca tcaaagcatc
     5761 atctacagtc ataattcctt ttataataaa aggtaattta gtagattcaa ccaattctct
     5821 aatctcatca attgtcttag gagaaaaagg attttcttga aattgattat taataagtcc
     5881 acaagcatca aggtctacac caactgcaaa agcacctgct tcttcagaca atctaatttt
     5941 ttctattatc tttgaattat tccaaggctt tataaataca attccatttc cactattatc
     6001 tttcaacaca tctaaattat ccagtaaaaa agtatctaca ttagtatcgc caaccatagc
     6061 ataaattcca gaattagaac aacctctaac aacaggctct atgtattctt tttcggaaac
     6121 tttaccaccc atgttcaata gagtacctga tacaggtgca gcgaatatag gagaactcat
     6181 ttttctacca aataattcta tggatgtatc tggctttgaa acattatgta taactctcat
     6241 atttatcttt attttttcta gactttttct attttcaatg aaagacgaac cacttccttt
     6301 accacccata ccaggaacat ctccagcaca agctaatcca ttacaaattt tacatacttt
     6361 acatgttcca ttaaagttct ctctagctga ttttagtaag tcattgtaat tcatacaaaa
     6421 tctctccttt ttataatatt aaaattgagt attactatat tgaaaatatc ttaattgtaa
     6481 atatagggat gttattatga tgagctaaac tagataatac atattagata ttttttattg
     6541 ttgtagatag ttaaattata tcatattaga tagtcaaatt ttgaaaaaaa actaacttat
     6601 ttaagtatac attttgaaaa aattaaaaaa gctatatata gatttttaaa gaaatctata
     6661 tatagctttt aagtttatgt caatcttttt ttattaatga ataaataata tagaatacaa
     6721 tatacaaaaa agtttcttaa aatgctaaga taatccccat ttacaaagca acatcattat
     6781 actgtgtaaa tttgaatcaa caatgttttc accatatttc accataaaat cttctaattg
     6841 tgtgtcaata aatgaataat atccacttat actatcgatg tatgcagtca ttaagtgtat
     6901 acattcatca cttttatctt tatacatata tatctccccc tatatacaat atataaattt
     6961 agatactaaa tcgtatcata ttaaatagtt aagtgtgtaa catctatgca gttatatatt
     7021 tatatcctta tattaataac taaagtgagt tttgacaata gattttggga aactggatgt
     7081 tcaaagtgta aacttggatt ttaaaatcat taattaagat agtgtatagg aatatatgta
     7141 tacataactt aattataaag attataaaca attactttta ttaactttat ttaagtggta
     7201 gaataacttg gagattaata attataagga ggatataata tgagtttata caagtttata
     7261 tatgatgata aggaatattt attaaaagag gagaactgta gtgctttaat aaatgatgaa
     7321 gaaaatccag ttcaaggagt aagtatttca aaaataattg atatacttaa tgaagcagaa
     7381 gaagttgatt ttgatgtaga atattatcaa gaagcatgtc cattatgttt agaaggagta
     7441 aaagaaaaga aaaaattctt tcctttttta gaatatcact tttatatttt cagtaaagat
     7501 ggagaatatg taataagcaa tatatctgtt gattataaag gactatcttt taataaacta
     7561 tcaagggcaa ataaagttga taatagttat attgtaagtg tgataatttg tgaaaattgt
     7621 caggattata tagttcagat agaaaattgt atagtttagt gtaaagtatg tcttaattta
     7681 agagaaattt ctaaattgag acatcaattt ttttcaaaaa tagtatggaa aattactaaa
     7741 tttatgagat aatgtaagtc ggaggtgagt tacaatatat agtaaatatt tggaagagat
     7801 agatgataca attttagatt caatgtttaa actaaccttg aatgaaagat tattgactaa
     7861 taacgatttt gttgaaaata aaattggtgt agtaaagaat ttaattgatt gctataaaac
     7921 tgaacaaaat agtttaatgt tattaaaatc aaattttttg ctagctttat tatatggtat
     7981 tcaagggttt agtgaaaaaa tgaaagagta tattttgatt tcctgtaaat atattgataa
     8041 atgcttaccc aaagattata aatttttagc acgattttat tcacaaatag ctattttaag
     8101 tttaaaaaat gaagatgtaa ataaggcaaa tttatatatg gataaattta atcttatttg
     8161 tgatgaaaat aattttctaa ttgaagaaat tatttttaaa tcacaactta tttacattaa
     8221 ggcaagtaag tgtatagaat cttctgtaat aattaaagaa atagaaacat tgtatataaa
     8281 gataaaagaa attaacgatt ttaactgtaa aaaaatatat ttttttatta ttggtaaaat
     8341 atattttttg tttttggatg atgctctaat tgctaaagta aattttttaa aagcaagaaa
     8401 atatgcagat ttactaaagg atatgcaagt atcttcacta tgtaatataa agataggtga
     8461 gtgtgaatgt tcatttgaaa attatgaatg ttccatagaa tatttcaatg aagtaataag
     8521 caataaaaag tatagaaatg taaatataat tcaaaaatat agagcatcaa acaacataac
     8581 taaaatatta ataaagacta gaaattatcc tgtagcaata aataatttag caaaatctga
     8641 agtatatcta gaaaaaatta aaaatcagag cttaaaagaa gtagagaagt ttgatttatt
     8701 tataagttta gctatgtatt atgcaaaaag taatgaaaaa tcttttgaac aatcaatatg
     8761 ctatttagat agagctaaaa acatcttaaa tatgataaaa aatatagaag aatatacaat
     8821 atatgattta gaaataattt atcatcaggc aaagatatat tatatctttg gatattatga
     8881 caatgcctta aacacaaata aaaaactcct tgaaaaatct aaaaatgcta aaaattataa
     8941 ttatgtgaag ttagcataca agaatatata tttgtgtttt gaaaagattc aaaattatga
     9001 aatgagtatg aaatacttta aaatgtatta tgagttaaaa atgatgtata taaaagctcg
     9061 aaatagaaat tatatagatt ccttaaatta tagacatgag tatatagaaa cagaaataaa
     9121 taacatgaga aaagtaaaac ttgatttaga taaaaaaaaa tatgttgacc atttgacaag
     9181 tgcatataat agaacttttt tgaacgattt tttagaaaaa caagaagtta gtgaatattg
     9241 cacagcattt atgatagatg tagattactt taaagaatac aatgatactt atggacacta
     9301 taatggagat attgctctta aaaatataac tataataata aaaaaatatc taaaaaaaga
     9361 catgtttatg ataaggtatg gtggagaaga atttctgata ttatctgtat gtaaagatta
     9421 taaaaaaagc aaaatatttg gaagaaaatt atgtaggatt gtaaagaaat ttttaaacaa
     9481 taatttgaca gttagtattg gcattgatac atgtaaaaat agtagtatag atataagaga
     9541 aataattgaa aatgctgata aagctttata taaagcaaag gaaagtggta gaaatagatg
     9601 tttgcattat catgatttta agaattttta actgttgaaa taggattggt gatttacttg
     9661 aaaaggagtt taatagaaga tttaaagaga aatataaaca agatagaata cgatatgttg
     9721 actaaattct atctaaattt gacagattta aactacatct tggattatca caaagacctt
     9781 ttaaatacaa aactagaaga taatagtaga gtctatactt tatatttaat tagccttata
     9841 aactatataa aaaaagatag agaagcatca tacaactatt tgatagagtc attaaagtat
     9901 attaaaaaat tgtcattaaa agaagattca gatataatat ctaaaattta tatttatctt
     9961 actttatcag ctatttctat aagaaaatat gataaggcaa ataattttta tttctcagca
    10021 aaaaaagtta taagacaaaa tcatttaaat gagttgctag tcttgttaaa tatgagttta
    10081 tgtgtagaaa taagtgcttt atatcagacc aaaacggatg taatagcttt agtagaagaa
    10141 gtgtcttttt ttaaaacttt aaaaaataaa gctcttgtaa gtagggcaaa ttatatattt
    10201 gggagagttt atctatattt atttaataac tttataaaaa gtatggagta cctaattgaa
    10261 tgtctaaaac tggctcaaga aaataattta tattctatag aggctttttg taaatgtata
    10321 ataagtttgt gttatttaga gagtggaaat aatgtagaag ctataaaata cataaataat
    10381 gtattagatg ttgcgcacag taagaactca atatcagtaa ctgaaagagt gtctataaag
    10441 atagacctta tatgggcata tttaaaagaa gatatgaata aagaagcaga agttatttta
    10501 ttaaagacta taaaattaat gaaagttgtt gaaggtgtat ataaggatta tttatattca
    10561 tttatatttt tatattcagc tgaaattgag ttaaggaaag ataattgtag ttttgaaaag
    10621 gtaaatacct atttaatttt atcaaaaaat atatataaaa gttttggcga tgtttttata
    10681 tataattcta aactagatta catatataag ttgtatggag atttatatat aaaatttggt
    10741 aatatagatg aaggtataag ttgttatata aaaggtttaa atttagttaa aaagagttca
    10801 ttgaatctta gaaaaatatc tatattttat gggctaatag caaatggata tgagctaaaa
    10861 aaagatttta aactggcaat agaatattat aaaagtatgg attggtattc tgataggtgg
    10921 gaaaaaaata attcctataa aacatctcaa gcaattcaca aacaatatga actcagacaa
    10981 aaacaagaga gtttaagatt actagtggat gataataaga aattagaaaa tgatatttta
    11041 aaagatggac ttactaaact atataataga agatatttgg aacagcagtt ggatttattt
    11101 aatggaacca gagacaaaaa tatggttgca atactagtcg atatagattg ttttaaaagt
    11161 tataatgata attatggtca tctagcagga gataaagtta tagttaaggt aactgatatt
    11221 ataagaagtg tatttataga tattaccgaa catataatca gatatggggg agaagaaatt
    11281 ttaatactag tagattctat agaatcaatc ggtgttgaag agattgataa tatagaatta
    11341 tatattaaaa gagttttcaa acttcttgaa tttgagggta ttgaacatat atattctaaa
    11401 ataaaaaact atataactat aagtgctgga gtaagtataa aaagatgtcg tagtagaaaa
    11461 gatgttgaaa tattaattga agatgcagat aagaacctat ataaatcaaa aaaagcaggt
    11521 agaaatcaat acattataat ttaaaattga ggtgattttg ttgcgaaaaa aaataggaat
    11581 tatagcctct gatattgaat taaaagaaag aatagaagaa ttatatagag aagatgtaga
    11641 aaatggaact ataataatcg acatacttaa cctagactta atggaaaatc aaggtcgtat
    11701 acttgttgaa aaaggtgccc aagctataat tggtagaggt ggaggttata gtttagtaat
    11761 agatacagta aatgtacctg ttataccaat gaatatgaag tcaactgacc ttttaagagc
    11821 aatagaaata gcaaagaaat attctaaaaa agtagtattg attcttggag acaatgaagt
    11881 ttcttttgac tatgttggct ggagaaatgt cataagtact gaaataactg aagaatggtt
    11941 tgaatctaag tatgagataa gaagtaaagt tgtaaaatat atagaccaaa aagatgaagt
    12001 tgtaatcgta ggaggaggtt tggcatgttc ttttgcaagg caatatggaa ttgatagtgt
    12061 atttgcaact gctagtgatg aatctataag agaagctgta gaatattgta aaaaattatt
    12121 ggatacatta ggtgaagaaa agtttaataa tgaagtgtta cgaaatatac ttgatggtat
    12181 aaaagatgga gtaattgcta tagatagtaa tggtagtata attttgtaca atgaaagtgc
    12241 aaaaaacatg cttaaagtag agagaaaatg tgcacttaat aaatacatct tagatgtatt
    12301 tcctaaaatg gagtggatgt tagattgttt acatgaaaaa gaggatgtag aagatagaaa
    12361 gataagaaat ataaataatc taattgttaa tactaggaca acattaataa aagttgataa
    12421 tagtacttat ggagttttag gaataataca agatataacc aagctacaaa acttagaaag
    12481 aaaaataaga tttgacttga atcaaaaagg tctctatgct agatatactt ttgatgattt
    12541 tttatttaaa gataaattaa ctagagaatt tattgaagaa gctaaaaaaa ttggaaaaag
    12601 tgattataca actttacttt atggagaaag tgggtctggt aaagaaatta tagctcatag
    12661 tatacacaat ataagtaaaa gaaaagatag gccttttgtt gctataaatt gtgctactat
    12721 agcagaaaat ttacttgaaa gtgaactttt tggctatgaa gaaggtgcat ttactggtgc
    12781 tagaaagggt ggaaaaagag gactatttga acttgctcat ggagggactc tttttttaga
    12841 tgaaataaat agtctatcct ttaatattca gacaaaacta ttaagagtaa tagaggaacg
    12901 tcaaataatg agaattggtt ctgattatat aatacctctg gatataagga ttatagcagc
    12961 aacgaacgaa tctttaaccg aaaaaatagt tatgggtaca tttagagcag acttatttta
    13021 tagattgagt agtttagaga taaatatacc tcctttaaga gacagaagag aagatataat
    13081 acctttgttt aacaattttg taaatgaagt attaaaggat gatggtctca atggaataaa
    13141 cagtatagat gaaaattttg ttttgactaa agatgaaatg gataagctat ataattatag
    13201 ctggcctgga aatgtaagag agttaaagac aatagctcaa aaatatgttg tgacaggtaa
    13261 aattaaattg agacaagata gagattttaa aactaaaaaa ttacttccta actctgaagt
    13321 agacaaattt aattctgaaa cgacagcaag tgtagaagtc caagatgaat ctataaatat
    13381 aagtaagata aatgatggta agataagtat agatataaaa gaagtaaata agtatgttga
    13441 agaaaaaata ataagtatgc tttttgctca gggactttct aaaaatgaag ttgcacaagt
    13501 tcttggaata agcaggacat ctttatggaa gaaatacaat aaaaatattt aaatataatt
    13561 acatataaat tattttgaag taatgttaat tttttaaaac aagtgttaag aaaaattaac
    13621 attacttttt tgtaaatttt tattttttta gacaggcata tatatttata agaaaaatag
    13681 ttaaaacact taatttaata atgaaaaaaa taataattct aaacaaaatc aattttacat
    13741 aaagtgggat ttattggcat agtaattgct taatatataa tgtaagagta aatacaattt
    13801 taggggtgat ggtatgagtc aaagtatggt tggtaagcat gcaatgtggc caaaagaaaa
    13861 tgatgttata tttagcatat ctggtagagc tcaagcagca gagaaggctt ttggtatgga
    13921 taatgtaata aatgcaacaa taggcgctct aatggatgat tcaggaaaat taataacaat
    13981 gaaaactgta tatgaagaat ataaagcatt agataactgt gagataggtg catatgcagc
    14041 tcttgaaggt caaccagact acttagaagc agttaaaaaa gtatttttca gagattactt
    14101 accagaagga catataagag tattagcttc accaggagga agtggtgcaa taaaattagc
    14161 tgtatggaac tatactaatg aaggagatga ggtattaaca tctgattggt tttggagtcc
    14221 atatgtaagt atagctgaag aggctaatag aaaagtagtt aattaccaac tttttgatga
    14281 aaatagaagg tttaattttg agtcgttcaa agaaaaattt gtaaatatag cagaaaaaca
    14341 aggtagagtc tttacaatta taaacacacc agcgcataat ccaactggat atagtgttgc
    14401 agatgatgag tgggataaaa tattagactt atcaaaagaa gtcgcaaaag ataaagacaa
    14461 aaagataata tttttcgtag attcagcata tattgacttt gcaggtgatg atgatgtatg
    14521 tagaaagttt tttaagaaat tttctaattt gcctgaaaac gttctcgtat tggttggatt
    14581 cagtatgtca aaaggattta cagcttatgg tatgagaatg ggggctataa tttgtatatc
    14641 ttcaagtgaa gatgtagctg aagagttcca ttactcatgt gttcattcat gtagagcaaa
    14701 ttggtcaaat tgtaatagaa gtgccatggc agtattatct aacattgtaa atgaccctaa
    14761 aaaattcaaa gaatatgagg atgaaaaaga aatatacaaa aacatgctta ctagaagagc
    14821 agatgtattt gtagaagaag ctgaaagagt aggattagaa attcttccat atatagcagg
    14881 tttctttgta agtataccat gtgataatcc aaaagaagta tgtgaagaat tgactaaaca
    14941 taatttattt gcagttccat taaaaatggg cttaagattt gcagtttgtg cagtttctga
    15001 agataaatgt aagaaagcac caagtattat aaaagaagca cttgaaagtt tagaagtaaa
    15061 aataaataat taatttcatt gaacaaattg ggtggactat gaatttaata attgtagatg
    15121 gagggaattt tatatgaaac aattgatgac aggtaatgaa gcaattgcac gtggagccta
    15181 tgaggctggt gttaaatatg catctgcata tccagggact ccaagtacag aaatattaga
    15241 aaatatagca acatataaag atgcaatagt tgcagagtgg gctccaaacg aaaaggtagc
    15301 acttgaggct gctatagggg gttcaattgc aggagcaaga acaatggctt ctatgaaaca
    15361 tgttggtttg aatgttgcag ctgatccaat atttacttat gcatatacag gggtaaatgg
    15421 tggtatggta cttattacag cagatgagcc gggaatgcat tcatctcaaa atgagcaaga
    15481 taataggatg tatgcaaaat ttgcaaagat acctttattt gaaccatcaa ctagtcaaga
    15541 agctaaagat atgataaaag aagcttttga agttagtgaa aaatacgata caccagtttt
    15601 atatagagtt acaacaagac tttgtcattc aaaaggatta gtggagtgct atgatagaga
    15661 agaagttgaa attaaagagt atgttaaaaa tgctaagaaa atggtaactg ttccagcaaa
    15721 tgctcaaata agaaggggcg ttgttgaaga aagaatggaa atccttaaaa aattctcaaa
    15781 tgaaactgat ttaaactact atgaaatgaa tgatacaaaa ataggtgtta tagcttctgg
    15841 tatgtgttgt aattttgcca aagaagtttt tggaaaaaat gcatcatata tgaaactagg
    15901 atttacaaat ccattacctt atgaaaaaat aaaagaattt gcagaaaaag ttgataaaat
    15961 atatgttata gaagaaaatg acccatttat agaagaacaa ataaaagcat atggaataga
    16021 ttgtattgga aaagatgtaa taccaccata tggagaaatg acaccagatg ttttaagaaa
    16081 agctatattt ggaaaaacaa atgatactat agaatacaaa tctgaattag taacaccaag
    16141 accaccaagt ttctgtgcag gatgtccaca tagaggattc ttctatgaac ttggaaagag
    16201 aaaaaattta atagttggtg gagatatagg ttgttatact cttggatttg ctcctccata
    16261 taatggaata gattatgttg tttgtatggg ttctgctttt ggaactgcac atggagctca
    16321 aaaagttctt aatatgaagg atgataatga aaaaagattg gtaggtgtac ttggagactc
    16381 aacattcttc catacaggaa taaatggact tttagatgtt gtttacaata gaggtaattc
    16441 tatttcggtt atattagata atagaataac tggaatgaca ggtcaccaag aaaacccagg
    16501 ttcagggtat actttacaag gtgctaaaac taaagaagtt gatatagaag gattagttaa
    16561 agcttgtgga atagaacatg taagagttat aaaccctaac aatttaaaag aagtaaatga
    16621 agcactagat tgggctttag ctatagaaga tgaaccatca gtaataataa caagatggcc
    16681 atgtgtactt aaaaaattct ctaaagaaga tatagaagaa tttaataatc catttaagac
    16741 taagtgtaaa gttgaccatg ataaatgtat aggatgtaaa ttatgtttga aaactggatg
    16801 tccagctcta tcatttgata aagagaataa gttatcaaat atagatagaa atcagtgtgt
    16861 aggatgtgga gtatgtgcac aagtttgtcc aaaacaagca atagttaagg aggaaaaata
    16921 atatgactaa gagtatactt ttggtaggtg taggaggtca aggaactata cttgctagta
    16981 aattattaac tatgggattg atggaagctg gatatgatgt aaagatgagt gaaatacatg
    17041 gaatgagtca aagaggaggt tcagtttcct cacaagttag atatggagat tgtgttcatt
    17101 ctccagttat agaaattggt ggagcagata tattagtatc ttttgaaaaa atggaagctt
    17161 taagatggtt caattattta aaaccagaag gaaaagcttt agtaaataat cataggatag
    17221 attctatgac tgttttaatc ggaggagctg aatatcaaga aagtgatata gatgcagagt
    17281 taagtagatt aaatgcaaaa gttataaatg cagctgataa agctgaagaa ctaggaaatt
    17341 caaaaataat gaatgttatt ttattaggat gtctagttaa atctatgcaa ttagagtcta
    17401 ttgattggga aaaaattata agtgaaaatg ttaaaccaaa atttgttgaa ttaaacataa
    17461 aagctttcca tgaaggtatg gaaatggtta gcaaatagtt tataatagat ataaatagac
    17521 tatagaaatt aaggtgaaag gaagaaaatt atgacttaca gaatattagc cataaatcca
    17581 ggttctactt ctacaaaaat agcagtatat gatggagaag aacaaattct tgtgaagacg
    17641 atagaccatc cagctgaaga gattgcaaaa tataatacta tacaagacca gtttgaaatg
    17701 cgtaaggaag cagttttgaa tattcttaaa gaaaatagta tagacttaaa atctcttagt
    17761 gcaatagtag gaagaggtgg agttttacca ccagtaaaat caggagcata tctagtaaat
    17821 gaagaaatga ttgatgtact aagacataga ccagtacttg aacacgcttc caatttaggt
    17881 gctgttgtgg cacatgcaat atcagaacct cttggaatca actcatatat ttatgattct
    17941 gttgcagtag atgagcttat agatgtagcg agaatatctg gactttgtgg aatggataga
    18001 tcaagtgcag ggcatgcatt aaatactaga gcaatggctt taaaatatgc taaggataaa
    18061 ggaaaagatt ataagagctt aaacttaata gtagctcaca ttggtggagg agtaagtatt
    18121 tatcttcatg aaaaaggaag aatggttgat atgctatctg atgatgaagg accattttct
    18181 ccagaaaggt caggaagagt acctgctaca aaattagtgg ctgcctgtta ttcaggtcaa
    18241 tattcagaaa gagaaatgac taaaaagata agaggtaaag gtggtatagt ttcataccta
    18301 aatactgtag atgctagaga agttgaaaaa atgatagcag aaggaaatga agaagcaaaa
    18361 attatttatg aagcaatggc ttatcagtta gcaaaaggta ttggagagtt agcaactgta
    18421 gtagatggaa aggtagatgc tataattata acaggtggaa ttgcatattc tgaaatgttt
    18481 acttcaatgg ttaaaaagaa agttgagttt atagcaccag tagaaattat ggcaggagaa
    18541 aatgagttgg aatcacttgc ttttggaact ttaagagtac taaatggaga agaagaagct
    18601 agaatttata gtgaaaatta ataagaaaaa ttaaaaatgt ttatatacta aaaaagtggt
    18661 atatccgttt tttatacacg aaagcttaga gtataggtga aaatgtataa aatttggatt
    18721 gccacttttt ataattaaaa ttttatctta atctaataaa taattctgta aaatttcgta
    18781 tttatatata tattttattg gtatttattg ttattattat tatgaggagt gatttttgat
    18841 gggaaaaata agtttaaaaa gtatgataag attattttca ggttttttta tatttgcaat
    18901 ttcttctgtg cttatgatta atgcacatgt aggtcttatg ccttgggatg tacttcacca
    18961 aggtttatct ataaagctag gcataactat aggtcaggct tctataatgg taggagttgt
    19021 aattgttata ttggatgcag tttttggtga aaatatagga tggggtactt tactcaacat
    19081 gacatttata ggaattttta ttgatttagt aatattttct ggagtgatac ctcatgcaag
    19141 caacacttat ataggtgtgt ttatggttgt tataggaatt attttggcag ctatagcaag
    19201 ttttctatac ttaggtgttt gtttaggaag tggaccgaga gatgggctta tgattgctct
    19261 tcagaaaaaa actaataaat ctgtacgtct tgttagaaca attcttgaaa tactagcatt
    19321 agttgttggg tggttattag gaggttctgt aggtattggt acgcttgtaa gtgctttagg
    19381 tttaggatat gtattacaaa tagtgtttag aatatttaaa tttgacacaa agttgctgaa
    19441 acatagattt attatagatg atataagaga atggaaggaa aagagaagca atgaacataa
    19501 atgtaaatca agtattgtaa taaaaaatga gcagaattaa ttatgctaaa atatatttgt
    19561 agtttatcta atttatagta aagtcaggag attttgaaat ggaattatgg gatttatata
    19621 atgctgatgg aattaaaaca ggaaatgtta taaaaagagg taattctatt gaagaaggtt
    19681 attaccattt ggcagttgaa gtgtggattt taaatagtaa ctctcaaatt ttaattcaga
    19741 aaaggtctaa aagtaaaaag acattgccaa atatgtgggg aatgacaact ggatgtattg
    19801 tttctgggga agatagttta gaaggagcaa ttcgcgaagc aaaggaagaa atagggatag
    19861 atataactaa agatgagatg aaagttttta gaagtatgat tcatgaagat actctttggg
    19921 atgtatactt agttaaaaaa gaatatgata tatccaaagc tattttacag gaggaagaag
    19981 taagtgatat aaagtgggta agcacagatg aaataagaca attactaaaa gagggtttgt
    20041 tttttgaata tccagagatg tacgaattgc tatatgagat agataataat aaattaaata
    20101 tatgatgtag agcagatttc agtataagta agattattta aaatgtatct tctctaaatt
    20161 ttaatattgt tttatgaaaa ttaataagtt atttttagta tttttctact tgttttttga
    20221 gattaaaata aaaagggcag tcattataaa gtcataaaaa tttggggagg atttaaacta
    20281 tggaagataa caaaggattg gcacgtgata gttcaaaata taaaattgtt tctatatctg
    20341 tcgtagtaat tattctaatt acattttggt atatgttaaa tatggtgctt ttaacattta
    20401 ttatgacttt tattttctat aacttgcttg tagccacaag aaaacgaatt aagaagtttt
    20461 cttctttaaa tattccagat tcattgataa ttatagtgtt atatgcacta tttgctatat
    20521 tgttagtatt gattagctat gcagttgtac caataattat agttcaactt acagagctga
    20581 gtagggtttt tagtgatttt gatgtaaatc aatttgctca atctttagga ccaaaattgt
    20641 atcctattgt ctcaaagctg gattttaata aatatatttc acaagcaggg ctacttatag
    20701 catcaacagc aacaaaagtt ggaagttttg gagtaaatat attattggca tttttactga
    20761 gtttactcct tttacttgaa aaaaatgaaa ttaaaaattt tggagataaa ctttctgata
    20821 gtaagatttc ttttatttat aattcgcttg tattttttgg aaagagcttt gttaaaaatt
    20881 ttggtgaagt aatgaaagta caagttatga ttgcctttat aaattctgtt gtttctatga
    20941 ttttcttagg tttcatggga ttccctcaaa tatgggcttt aggatttatg atttttgtgt
    21001 tgggtcttat tccagtagca ggagttatag tgtctttgat tccattaact gtaatagcat
    21061 ttaatacagg aggaataaca aaggtttttg gagttttact tatgatttgt atagtgcatg
    21121 cagtagaaac atacatatta aaccctaagt tgatgtcgaa tagaactaaa cttccagttt
    21181 gttttgtttt tattatttta cttgtaggag aacattattt aggtgtatgg ggattactaa
    21241 ttggagttcc aatatttatg ttccttatgg atattctggg tgttaaattt acttctaggt
    21301 aatatattaa aataataatt tatttttata tataaattat gaattgtttt aaaaaagaac
    21361 ctataaatag acatcattaa taaagacatc tatttgatag gttcaatttt gctttgtata
    21421 aatactatag aattaaataa aaagaaattt atttcatttc aaaagtttca caacttgcat
    21481 ttctttcatt tatatgaact ttttcagctt tgcaaagttc atttttattg tatttacatt
    21541 ttacagcttc acaatgaatg ttatctgtat caacttggtt agtttcatta gaacgactag
    21601 ttataccaga tgtatatttg tcttcaaaag aagcacaata agtattttct gtagtagttg
    21661 cttgcattcc atctactttt atactaccag cataacaata tccactagta ttataagcac
    21721 aattagtagc agcacaattt aaattattat ttttcataaa taacctccta agttttattt
    21781 aaacttatta tgtgaagtta tctaaatttt atacacttat ttacaattac atttatcaca
    21841 tttaacaact acatctttgg aagtttccct tcctaaattt ttattaacat gatacacatc
    21901 aacattttca gttgatccac atatagaaca tttaaaattg ttttctagta gggttctttc
    21961 tttaaataac ttccaataca tagaattgat gtaatcatta taatttacct tgaggcatgt
    22021 ctcaagaaag tttatgtata gattgtctaa atctcctgta tttaattctg tatattctgg
    22081 ttcaaaatat tcacaacatc ctctaaaatc aggctttgta tctgaaataa cacatctaat
    22141 atcatatcta tagtctaggc taggcccctt aaatgaaaga tgtgtgcaaa aagaacaaga
    22201 tgtttctggt agattttttt caagttcatg taatttatta ttcataagta ttcctccaaa
    22261 aaatttattt taagtatata aatgaataga ttattttaaa acttttattt atttatatta
    22321 gagatttagg ataaatattc caataaggag taaatactta agtatttatt actgtattaa
    22381 ataaaaaaga ggcaaatcta acttgagatt gcctactttt ttatcatttt ttaataaaat
    22441 ttattctaag tattatttta tttagattta gtcagccaat ttagtatccg ctttgctttt
    22501 taactttcta ccagagcgta cagttaaaat agcatataat acagtaaata tggcagctaa
    22561 aacatatgat atatttatgt ttaagttaaa tccaatttga gcattaaata tataactgaa
    22621 tataacaaaa gaataaaaca taccaggaat taagcttata atataattct tttcttttat
    22681 tataaggtaa actgtaatca ttgcaaatgc aaatatggca atagtttgat tactccaaga
    22741 gaagtatctc cacaatatat taaatccaga agcactaagc tttgcaaata taagaatggc
    22801 tatgacagga ataaatatac agattgacaa tataacacga tgtttctttt ccttttggtc
    22861 ataatgaaag taatctgcaa gcattaatct taaagatctt aaagctgtat ctccagaagt
    22921 tattggtaaa acaattacac ctattatagc tatgatacca ccaatagaac caagtaaatc
    22981 tcttgcaacc aaacctatta catcaggaga acctactaat tccttaggta ttcctttatt
    23041 gtaaataccc atagcagctg cagcccaaat cattgcaatt aaaccttcta aaatcatcat
    23101 attataaaaa gtagtttttc cttctttttc atttgaaact gatcttgcta taagagtagc
    23161 ttgagttgag tggaatccag aaacgatacc acaagcaact gtaacaaaga atgtaggaat
    23221 aagaggaata ccatcaggat gaataccttt ccaatttgca agacttaaat tagctaaatc
    23281 ataaccttgt gtaaagattc caattccaac accagcagca gataaaagta gaatagctcc
    23341 aaatattgga tatacctttc ctatgatttt atcaattgga aagagtgttg cacacaagta
    23401 atatagaaag ataagtccat aaactatcca gacaactgga ttatttatag tcgatttcat
    23461 atttaaaatt tgagtaacaa ctaaatcccc aggtgtataa ataaaaactg caccaactaa
    23521 aagcattaaa agacaaagga atatattata gacttgaaat actttattac ctaaaaatct
    23581 actgataaga gaaggcattt gtgctccttt ttctcttata ctaatcattc cactcatata
    23641 gtcatgcaaa gctcctccaa atacacagcc aataggtatt agtatgaatg ctattggacc
    23701 aaacaatata ccttggatag gtcctagaat tggtcctgta ccagctatat taagcaactc
    23761 aattagacta tttttccatt ttttcattgg gacatagtct actccatcac tttgagcaag
    23821 tgcaggtgtt tttctgtcat ctgggccaaa tactttttca caataagcac cgtataaagc
    23881 acctcctatt agtaatatag ccaagccgat taagaatgtt gtcataatat caccccattt
    23941 ataaaatttt ttggtctttg tatacatatt atctaaatgt gtagatttat attgaattta
    24001 attctggaca acctacaaat tctataaagt attacaacta atataatttc agaaataaat
    24061 acatatacat aaccatgtac ttagtataca ggtatttggt aatattttca atgttatagc
    24121 aataaagaat ataaacaaaa ttctgataat attttacaaa aaaattaaaa aatataaaaa
    24181 ttagtttaaa tagatggaga aaaaaggaaa atcaattgag aatataaatg tctatatctt
    24241 aacaatatta agaggtttaa aattcttgaa gaaagaatag tcaagtgata taatgggttt
    24301 acaggtgtat atacactagt acatatagaa agcggtgcaa ttatggataa agatttaaca
    24361 tatcaaatca aagagctaaa gaaagaaaaa aatgcaataa tacttgccca tttttatcaa
    24421 ccaccagaaa tacaagaatt agcagatgca gttggtgact catattattt aagtgagatt
    24481 gctagagatt gtaaagaaga agtggttgta ttttgtggag taaggtttat gggagaaagt
    24541 gctaagatat tatcacctga aaaaactgta ttgatgccag taagtaacgc tgggtgtgct
    24601 atggcagata tggtagatga agaaggcgtg ataaaattaa aacaacagta tccaaatgct
    24661 ttggttgtat gttatataaa ctctacagct aaagttaagg cacattgtga tgtttctgtt
    24721 acatcttcta gtgcaattaa aatactagaa aacatagata ataaagaaat tatattcctt
    24781 ccagataaaa atttaggagg atatattgca gaacaatttc cagataaaaa ctttatattt
    24841 tgggacggat attgtaaata ccataacaat ataagagctg aagaaataat tgaattaaaa
    24901 gataagtata aaaatgcaga agtattagta catccagaat gtaaaaaaga aataagagat
    24961 ttaggagatt atgtaggtag tacatccgga ataataaaat atgctactaa tagtaaaaac
    25021 aaagacttta taatagcaac tgaagaaggt atattacatg aattaaagaa aaataatcct
    25081 aataaaaact tttatattcc tggtggaaaa atactatgta cagatatgaa aaagacaaca
    25141 ttagaaaatt tatatagtac acttaaaaat atggaaaatg aagttattgt agaagatgaa
    25201 atcatggaaa aggctttaaa ttcactgctt aacatgcaca aattagcaga aggatagagg
    25261 gatagactta tgaatttaga acaagatgtt ttaatagttg gaagtggagt ttcaggatta
    25321 tactgtgcat taaatttaga taaaagttta aatgtgctag tagtatcgaa atcaacaatt
    25381 gaaaacaaca atacatatct tgcacaaggt ggaatttcaa ctgctagaaa tattgatgat
    25441 atagaatcct ttgtagaaga tacaatgaag gctgggcagt ataaaaatag agttgaagca
    25501 gttcaagtac ttgcagatga atctatagaa aatgtagggc agatagtaga atatggtatg
    25561 cctttagaca aagaaaatgg agagatagat tacacaagag aaggagcaca tagtgtaaat
    25621 cgaatagttc atagtaagga taatactgga gaagttgttt ttaaaacttt attaaaagaa
    25681 gctaaaacta gagaaaatat aactcttata gaagatgcat atcttttaga tatattaaaa
    25741 gatggcaata aatgtatagg agctagaatt tttaaatcta aaaaagagat tcatgttttt
    25801 tcaaaaatag tagttttagc tacaggtgga attggtgggc tttttaaaaa ttctactaat
    25861 caaagacatc ttacaggtga tggaattgca atagctttaa gaaataatat aaaaatagaa
    25921 aatttagaat atattcaaat acatcctaca gccttttatg aagaaaataa tgaaggtagg
    25981 agaatgctta tatctgaatc attaagagga gagggagcta aacttttaaa taaaaataaa
    26041 gaaagatttg tggatgaact tcttcctaga gatgtagttt caaaagctat ttttgagcaa
    26101 atggaaaaag ataaattacc ttatgtgtat ttagatgcaa cacatttaga tagtgaatat
    26161 ttaattaata gattttcttt tatttataat gaatgtttag ctagaggaac agatataacg
    26221 aaagaatgta taaaggtatc tcctgcacaa cattatttta tgggcgggat acatgtagat
    26281 ttggattcaa aaacatctat ggaaaattta tatgcagttg gtgaaattag ttgtacaggt
    26341 gttcatggag ccaatagatt ggcaagtaat tcattacttg aagggcttgt attttcaaaa
    26401 agagcagcta aaaatataaa ctcagttata gataatgtaa aagttaagtt tatagatgta
    26461 ccagatatgg atatagatat agaacaggta aaaaaagaaa ataaaatatt agtcataaaa
    26521 gaaattgaaa gaacaagtga ggattttggc gatgaattat ttgattattg atagaatgat
    26581 acaggatgct ttgatagaag atgttccaag tgaagatata acaactaatt ctattgtgga
    26641 tgaaaattct aaatctactg tagatttaat ttgcaaacaa gatggtataa tagctggctt
    26701 aggtgtattt aagagagttt ttgagatact tggtgatgta gatgtaaaat tatataaaaa
    26761 tgatggtgat agagtaaaga atcgtgaaaa gatagctgta ttaacaggta gtactagaaa
    26821 cttacttgta ggagagcgtg tagcattgaa ttatttacag agaatgagtg gtatagctac
    26881 tattacaagt aggtatgttg aaaagcttga aggtacaaat acaaaattat tagatagtag
    26941 aaaaacgata cctaatctta gaatattaga taagtattct gttaaagtag gtggaggatg
    27001 caatcataga tttaatttat ctgatggaat attgcttaaa gacaaccata ttggggctgc
    27061 tggtagtgtt aagaaggcag ttgaattagc tagaaaaaat acttcttttg ttagaaagat
    27121 agaagtggaa gtggaaactt tagacatggt aaaagaagca atagaagcta atgcagatat
    27181 aataatgttg gataatatga gtttggaaat ggcaaaacaa gcagtagata ttataaatgg
    27241 aagagccatt gtagaatttt ccggaaatgt aaatttagat actatagaag atataggaaa
    27301 aattggtgtg gatgttgttt ctgtaggagc gcttacacat tctgttaaag ctctcgatat
    27361 aagtatgaag aatttgagaa atatataagt tttatattga aataaactta attaaaatag
    27421 actccttaaa tttgttttat aaatttaagg agtctatttt ttattcatta aaattaataa
    27481 aaatataaaa aaatcaatca ataatttaat aaaaatagat aaatactata tacactttta
    27541 ataatatagc taatatctat tttacagata aataagaata aacttataat ataattgtat
    27601 ctgtaaaatt ttgtaaaata taaaaagtat agggggaata aaaatgaaag tagcttatat
    27661 ttttagtagt actaatacac ataaaattct agacaaaatg ataataccac aacttgaaca
    27721 aggaactcat ggagtagacg ttctagggat gtttttcttt atggataata ccttattttt
    27781 atctaaaggt aatccagtag gcgagagact aagtaaatta catgaaaaaa ctgatatgat
    27841 aataatggct tgtgaccaat gtgctataga aagaggtata gagaataatt tggttgatgg
    27901 agctacaatt ggatgtttcc caaacctata tgcttgttta ggcagtgcag gtgtggacca
    27961 agttataacg ttataaatta gtataggata caatccatag aaaaatttat tttctgtgga
    28021 ttattttaag taaaagataa attgtttaaa gtaggggatg aacaaatgtc taaaatttta
    28081 gttgaattta taaacacttg accatcttgt gatgaacatg gagtgtttat aaaaaaaatg
    28141 tcagaaaagt atgctgataa attggaaata aaattatatc aagcagggaa agatttttcg
    28201 tatataaaaa aatatgggat tataacaaaa ggaactttaa taataaatca gaaaaagaaa
    28261 tatgatagat taaataaaga tacaattgaa agggcaatag tagaagctat aaacaataat
    28321 taaaagttta aaaattagga gtatcaaaaa tgaatatatt agaaattgtt ttaaatggag
    28381 ttaaagatac tatagtagct tctatagtta cactgaatgg agcatcagga tggcttatga
    28441 taagttttat attggcaggt gtacttcaca atgttttaac tccaagaagg tttcaaaaac
    28501 aacttggaaa taagaaattt tcttctttaa ttaaaagtac tatctcagga atgtttttac
    28561 caatatgtag ttgtggagta ataccacttg gaataagtat gtattattca ggagcatatt
    28621 taggaccagt acttgctttt atgacatcaa ctccaataat aaatccaata gctgtagttc
    28681 tttgtctagg tcttttaggt cctaaagtta caatcattta tctaatagct ggttttttag
    28741 taccattttt agtaggattt ttaggaaatt gttttggaaa agaagaatta tcactttata
    28801 atgattcaga ggaagaagta atagaattag aagaagaaaa aagaagtttt atggaaaata
    28861 tgattgatgg tcttaaatgg tcttttggtg aattagccct tactattagt aaatatgtag
    28921 taatgggtat ggtttttgca ggatttataa caactatttt tccaaatagt ataattcaaa
    28981 aatatttagg aaatccaggt ttgctatctc ttggaagtat agcaatactt gcatgtgtta
    29041 tgtatgtatg tgctgtagga catataccgt ttatagcagc ccttgttgca agtggagcat
    29101 ctcctggtat agcgataaca tttctaatag ctggagcagc tacaaatcta ccagaactta
    29161 taagcatgtt taaacttatt ggaaaaagaa ctgttattat atattctgta actttgacac
    29221 tgtcctcaat aataattgga tatataacaa atttactttt agtgaataat aattcagtaa
    29281 atttagatgc aacaaattct tcaatacagt tggcaaataa actaatgtta aatatacctg
    29341 atagttttga gtatgtatgt actattttaa ttattttatt tgcatttaaa tcaacaatac
    29401 caaagatgaa agaggtcata ggaaatcatg cttaaaaaga tagttataat atcctgtata
    29461 gtagttgtgt taatcatttt atcaaaaata gttgatgata acatcaaaga agatgcttct
    29521 attccaaatg tgaataaaga aacattagaa tattttagaa aaaattataa agaagacata
    29581 ataacttgtg ctgaagaaga tttaaataat gatgggaaaa aagatttagt agttatatat
    29641 aaaaaaagca ataattcaaa tgaaatggta gtcgtagttt ctgataaaaa tagccactat
    29701 ataacaaagc caataccagc acctatagag aatcaaacta taacctttaa aaatattgat
    29761 gataaagcac caatagaagt aatagtatca gggtctaaaa atggtaatgt aggatatgct
    29821 atttacagag tagagggtaa aaaatttgtt gatttatttg gcgaagatat ggacaaatgt
    29881 tgctaaaaat aaaaataata cataaaggtg ggaaatttat atgaaattat ctaagaagat
    29941 tcaagcacta atcttatttg gtgtaatggg tacatctata ttaacaggat gtagtagtaa
    30001 accaaaagaa aaagaagaag caaaagctgg aaaagatgat tatacaataa cagtaggtta
    30061 ttataattgt gaccatatga ctgcgggacc agtagcagaa tcggcaggaa tatataaaga
    30121 tttaggtctt aatgtaaaaa ctgttggaaa tggtaaagtt cctgaagcta tggcagcagg
    30181 aaaaatggat gcaggttaca taggaactaa ggggttagtt ggagcaatac ctaagggttc
    30241 accaataaca atagcagcta acaaccatac tggtggttct gaatatttaa tagtttcaaa
    30301 agatataaaa gaacctaaag atttaatagg taaaaaaatt gcaacagata tgagtgattt
    30361 cttatggaca agtgattatg gtccagaaac aggtctacca actgacccat caaaatatga
    30421 agttgtaaat atggattcag acaaagataa atatttagct cttaaaactg ggaaaattca
    30481 agcttttaca tcttgtgacc cttggggttc tgtagctgaa aatgacggtg caggaaaaat
    30541 aatagcatct actcaatata aagaaaaagc taatggcaaa gaatataact gttgttcatt
    30601 ctcattaaac aaaaacttta taaaagaaca tccagattta gcaaaaaaat tggttctagc
    30661 acatacaaaa gcaatagaat atatatatac aaatccagct gaagcagcta aaatatttgc
    30721 aaaatattat aatgtagaag aagaagttgc acttagaact atctataaga agactgttgg
    30781 agaaggtaga actcttactt ggaaagttac aggtgaagaa tataaaaata atttacaaat
    30841 gtataaagat ttaaaggctt tagatgatat acctaaatat gaagatacga ttgatacaag
    30901 cttattggac tcttgtggag ctgatgattt tgacaaattt ataaaagaaa aagttaaccc
    30961 agagtttcct gaaggtatga gttatgaaga ctggaaagca aaggtattat aaataaatgg
    31021 agaatataaa aaatataata atacttttta tcattatttt gggaagtata tttataatat
    31081 tttttaattt tgaagataaa gaagaaataa aaggaaaatt aagaataggg gtatctgatg
    31141 atacctctgg ttttgtaata aattatatgg taaatagaga ctactttgaa aatataaaag
    31201 taagtgatat aatggatatt tttacaatta atgattgctg agcaagtaca atgcagtggg
    31261 ctctgagttc agaggaaata gatatagcaa tcatatgtaa agatgctgca aaacaatatg
    31321 taaatgtaaa tagtggattt gaaattgttg gtactgtagt tcaaaattct gatatatttc
    31381 tattaagtga taataatcct gaaaagatag gagtcattca aaatagagat taccagtcag
    31441 aattggttaa aaaatactac aagaatgtgg aagtagctcc attattaggg actgcactag
    31501 catatggatt agaaagtaat ttagtagatg gtgttgtaat agattgcata aagtcaattg
    31561 ggttagaggg taaaagaaaa tctacaacta aattaggaga ttatgatacc tatgtattag
    31621 ttgtaagtaa agagtttaaa aaaagtaaag catatagtga ttttataaca ctttacaata
    31681 aaagtgttga tgctttaaag aataaagcta catttaagaa agcattaaaa gagtacagga
    31741 atataagaaa tatagatatg gaggaaataa gcaattggaa attaaaattt ctaaagataa
    31801 aagcgataga ttaactaaga aaaaaactga tggtagtatg gatattttat ataaaataat
    31861 actattagtt attatatttt tagtctggca ttttgcagca aaagatatag ggagttcttt
    31921 gttacttcca atgccagtag atgtaataaa aggtttcttt ttctgtgtga cagatgcaga
    31981 gacagtcact aatcttttta taacattgca gagagtactt aaaggtttta tgtatgcatt
    32041 attgtttggg cttccaatag gatttattat gggattttct aaaacttttg aaagggtttt
    32101 atctccagta gtagattctg taaggcaagt tccaataatg gcatgggttc ctcttactat
    32161 agtatggttt ggtataggag atggtccaac aatattttta atagcttttt caggggtttt
    32221 ccctataatt ttaaatacta ttcaaggagt aagagcaata tcaaaggatt attataatgc
    32281 agctagaagt atgggagcat cgccaattgt gatatttaca aatgtaattg ttcccgcatc
    32341 tcttccagat atactaactg gttcaaggat tgctataagt acaggatgga tgtctgttat
    32401 ctgagccgag tttatagcta cgagtgccgg tctcggttac tcaatggtac aagctcaaac
    32461 attaatgcat acagatgtat taattgggct tatgatattt gcagctttaa taggatttat
    32521 aatagatagg gtattaaagt ttataaataa aatcctttgt agatggaggt ttgcagatta
    32581 atgaagttaa gtgtaagaga tataaataag acatttgtaa ataatagagt acatacaaag
    32641 gttttagaag atataagtat ggatatagat gatggacaat ttgtctgtct tttaggacca
    32701 tcaggttgtg gaaaaacaac tttacttact ataataggtg ggtttcaaaa atcagaacgt
    32761 ggagatgttt ttataaatga taaaagagtt aaaaaaccgg gcatagatag agcttttata
    32821 tttcaaaatt atgctttatt tccttggaaa actataagag gtaatgttct atttcctatg
    32881 aaacagcaga aaataccaaa agaaaaaaga gaagaaatgc tagaagaact tttggttatg
    32941 tcagacttga aaggaaaaga aaaactgttt ccgcatcaat tatctggagg aatgaaacaa
    33001 agagtggcca tgataagagc tttagcatgt aatccagagg tattactaat ggatgaacca
    33061 ttaggagctg tagattttca aatgagacaa aatttacagg aagagctaga acgaatatgg
    33121 ataaagaaga aaataactgc attaatggta actcatgatg ttgatgaagc tgtatatatg
    33181 agtgatagag ttatagtaat gtcgagagat aaaggaagaa ttattgatga tataaatata
    33241 gatattccaa gacctagaat tagaggaagc caaaaatatg aagagtacaa aaacaaatta
    33301 actgatactt tatcaaagtg ttatgaagtt taaagaggag tagacatgag tcaaatagaa
    33361 aaatacaata gtttggataa tacacaacag aaaataagaa tctctattat ggatatgata
    33421 atagataaag gttctagtgt tacacttcaa gaagtgactg aatatgtaag taaaaagttg
    33481 aatatagaaa aagaatatat agaaaggaca ctacaatatt ttatttatga aaatataatg
    33541 gttgttgatg ggaatagtat aaattttata tatcctgtat cagcattgca aacaaatcat
    33601 aaagtaactt tgagagataa tagaagtttc agcgcaatgt gtgctataga tgctatagga
    33661 acctcttgca cattcaacca agatataaaa ataaactcta tttgtagtgc tacaggtaag
    33721 gaaatagaag taattataaa aaatgaaaag attgagtatg tgaataatcc aaacttaaga
    33781 gtgcttcata taaatcttga taagcatcta aattgggctg ctagttgctg aaatataatg
    33841 aatttcttct ggacgaagag tgattttgat gcttggacaa acgaagcggg attatctgat
    33901 gatgaagata tatattgttt agatattaat gaggcaatag tagaatcata taaaatattt
    33961 aaactcaaac aaaaggtact atcatagata tacatatatg aaaaactaaa aatgaccttt
    34021 agataaaaat gataggtaat atccatacta tataaattag tatagagtag ggatgtgaaa
    34081 agtatttaaa tctaaaggta tatttttagt ttttttagat gattagatag ccttattaag
    34141 tatatattta tttataacct ttgcaactcc attttcatta ttagtatcag taacataatc
    34201 tgcaatttcc tttatactat tacgtgcatt ccccatagcc acaccaagac ctgcatactt
    34261 tatcatatgt tggtcatttt cttcatcgcc aacagcaata gcattttcta taggtatatt
    34321 tagattcgta cataatgttt taagagcagt tcctttatta gtagttttac ctaaaaattc
    34381 tagataaaaa ggagcagacc ttactatagt atatttattg aataaatttt taggtagttg
    34441 ctgtatgatt tcttccaatc tttctggttc atcaactatc ataattttac agattttaat
    34501 agattcatca attttatctt tttgtatata tttgacttct atattattta atttcgcttc
    34561 atgaagtgta tattttccag gtgcttcatt tggtgtaatt aaatttgtca ttgtatttat
    34621 gtgtatttta gtcttatatt ttttacaaac gttattatat ataaaatcaa aatcatctaa
    34681 agtcatttct acatcatgta tgattttctc acttttaact tcttgaataa ttgctccatt
    34741 aaagcatata acataatcac cttcatctgt taaatttagt tgatttagaa gttcttttac
    34801 accaggaaga ggtctgcctg ttgttataac tacttttgcc ccagcccttt ttgcctcctg
    34861 aatggactca aagacttcag gagtaatcct tttttgtgta tttaatattg ttccatcaat
    34921 gtctagtgcg attaatttgt acatatcaac ctccaataaa ttatatactc aaatttcctg
    34981 tgaaaacgtt gacacagcta gtgttgaaca aagtttcagt gggaaatatg gtttgttttg
    35041 tatactatta tatcaagaga taagtaattt agaaagttta ataatattaa tttttgataa
    35101 aaatattact tatgtgagat gagaatgtaa aaactttgta atcaataatt ccaaaattct
    35161 taattacaaa taatggtatc gtttgcattg ttaatataaa taaatgtata ttatatagtt
    35221 agaagtttga ataaatagac tgatggtaat ttgttatatt agttagtttt gtgttataaa
    35281 atattgtaat tccattaatc atatgttatt attcaacatg attataatat aaagaatatt
    35341 taatatatat
//
LOCUS       DAGQWR010000023        34415 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.023, whole genome shotgun sequence.
ACCESSION   DAGQWR010000023 DAGQWR010000000
VERSION     DAGQWR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34415
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.023"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(308..820)
                     /locus_tag="MIW95_000995"
     CDS             complement(308..820)
                     /locus_tag="MIW95_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150341.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEDYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKAKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            1373..2254
                     /locus_tag="MIW95_000996"
     CDS             1373..2254
                     /locus_tag="MIW95_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBY3150342.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKKGVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(2567..3346)
                     /gene="hisJ"
                     /locus_tag="MIW95_000997"
     CDS             complement(2567..3346)
                     /gene="hisJ"
                     /locus_tag="MIW95_000997"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBY3150343.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEDLIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(3886..4236)
                     /locus_tag="MIW95_000998"
     CDS             complement(3886..4236)
                     /locus_tag="MIW95_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBY3150344.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(4252..4668)
                     /locus_tag="MIW95_000999"
     CDS             complement(4252..4668)
                     /locus_tag="MIW95_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBY3150345.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(4687..4878)
                     /locus_tag="MIW95_001000"
     CDS             complement(4687..4878)
                     /locus_tag="MIW95_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBY3150346.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(5029..5844)
                     /locus_tag="MIW95_001001"
     CDS             complement(5029..5844)
                     /locus_tag="MIW95_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBY3150347.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(5891..6829)
                     /locus_tag="MIW95_001002"
     CDS             complement(5891..6829)
                     /locus_tag="MIW95_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY3150348.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDASNKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYIYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(6826..7698)
                     /locus_tag="MIW95_001003"
     CDS             complement(6826..7698)
                     /locus_tag="MIW95_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY3150349.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEK
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(7723..9651)
                     /locus_tag="MIW95_001004"
     CDS             complement(7723..9651)
                     /locus_tag="MIW95_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBY3150350.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNSL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(9763..10140)
                     /gene="gcvH"
                     /locus_tag="MIW95_001005"
     CDS             complement(9763..10140)
                     /gene="gcvH"
                     /locus_tag="MIW95_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBY3150351.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(10227..12353)
                     /gene="cdhC"
                     /locus_tag="MIW95_001006"
     CDS             complement(10227..12353)
                     /gene="cdhC"
                     /locus_tag="MIW95_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBY3150352.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(12410..13216)
                     /locus_tag="MIW95_001007"
     CDS             complement(12410..13216)
                     /locus_tag="MIW95_001007"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBY3150353.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(13266..14633)
                     /locus_tag="MIW95_001008"
     CDS             complement(13266..14633)
                     /locus_tag="MIW95_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBY3150354.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(14664..15608)
                     /locus_tag="MIW95_001009"
     CDS             complement(14664..15608)
                     /locus_tag="MIW95_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBY3150355.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(15628..16398)
                     /locus_tag="MIW95_001010"
     CDS             complement(15628..16398)
                     /locus_tag="MIW95_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY3150356.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(16454..17839)
                     /gene="lpdA"
                     /locus_tag="MIW95_001011"
     CDS             complement(16454..17839)
                     /gene="lpdA"
                     /locus_tag="MIW95_001011"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBY3150357.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVIEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(17883..18764)
                     /locus_tag="MIW95_001012"
     CDS             complement(17883..18764)
                     /locus_tag="MIW95_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBY3150358.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMQKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(18800..19474)
                     /locus_tag="MIW95_001013"
     CDS             complement(18800..19474)
                     /locus_tag="MIW95_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBY3150359.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(19511..20383)
                     /locus_tag="MIW95_001014"
     CDS             complement(19511..20383)
                     /locus_tag="MIW95_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBY3150360.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(20475..21107)
                     /locus_tag="MIW95_001015"
     CDS             complement(20475..21107)
                     /locus_tag="MIW95_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBY3150361.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKIEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(21212..22888)
                     /locus_tag="MIW95_001016"
     CDS             complement(21212..22888)
                     /locus_tag="MIW95_001016"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBY3150362.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(23137..23916)
                     /locus_tag="MIW95_001017"
     CDS             complement(23137..23916)
                     /locus_tag="MIW95_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY3150363.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(24226..26145)
                     /gene="cooS"
                     /locus_tag="MIW95_001018"
     CDS             complement(24226..26145)
                     /gene="cooS"
                     /locus_tag="MIW95_001018"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBY3150364.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(26752..27663)
                     /locus_tag="MIW95_001019"
     CDS             complement(26752..27663)
                     /locus_tag="MIW95_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBY3150365.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(27779..27901)
                     /locus_tag="MIW95_001020"
                     /pseudo
     CDS             complement(27779..27901)
                     /locus_tag="MIW95_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            complement(27984..29126)
                     /locus_tag="MIW95_001021"
     CDS             complement(27984..29126)
                     /locus_tag="MIW95_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBY3150366.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYKVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(29144..30898)
                     /locus_tag="MIW95_001022"
     CDS             complement(29144..30898)
                     /locus_tag="MIW95_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBY3150367.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMKINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            31034..31240
                     /locus_tag="MIW95_001023"
     CDS             31034..31240
                     /locus_tag="MIW95_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150368.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(31245..32168)
                     /locus_tag="MIW95_001024"
     CDS             complement(31245..32168)
                     /locus_tag="MIW95_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150369.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(32422..34122)
                     /locus_tag="MIW95_001025"
     CDS             complement(32422..34122)
                     /locus_tag="MIW95_001025"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBY3150370.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
BASE COUNT        10447 a         6764 c         3760 g        13444 t
ORIGIN      
        1 tttttgacgc aatcgttttt attcagtcat tttatattct tgatacaatc attcttacat
       61 tctattgttg tttgttttta acacaatcgt tttttgttga atcattttat attcttgata
      121 caatcattct tacattctat tattgtttgt ttttgacgca actatttttt attcagtcat
      181 ttaatatttt cgacacaatc attcttacat tctattattg tttgtttttg atacaactgt
      241 ttttattcag tcatttaata ttctcaacac aattaccttt tcattcaatc aaagtaatct
      301 ttaagcatta tttttcaagc tcatcaaagt aattatatac gataacctca cctgtctctt
      361 tatctgtagt ctttataatg cccttagctc catttacctc ttcttttgta aattcatcat
      421 ctaacacaac tttgaattta tcttcctttc cataaccttg ttgaaattca tctctcaaaa
      481 ttgcattttt ttctgctttt gccttcaact caaattcaat cccagatttc atttcatcaa
      541 aataatttaa tactacaacc tcattattca ttttattagt tgtctttata atccctttag
      601 ctccatttac ttcttctttt gtaaattcat catccaatgt tatattaaat ttgtcttctt
      661 ttccataatc ctcttgaaaa tccctcttca tttcattgta tttagtttca aactctttac
      721 tcttgtttaa ttccatagct gatacatttg tcaatgatgc tccaattgcc attactgtta
      781 atgccattac tataccactt ttaaattttt ttactttcat accttacctc ctaaattaaa
      841 tgttctcaat gaaattatat aaagaaaatc ctaagtttta cagtttcatt cttgtaacta
      901 aaattactat tatgtaacca aactttttgt ttcaactatt ccttaaaagt tgctttttta
      961 aggaatagtt gaagttaaat aataagattt tatctatatt gatagattta aaatcaaatt
     1021 tatccatata aataaatact tatttttgca aaataaaaat ttttaattac tccaatcctt
     1081 cttatttact taaaatcctg tgataaaaaa tttaaaagtc tttacattct ttaacaataa
     1141 tttatttact tttaacatac aaaatatata gagtatatca aaattatttt actgaatatt
     1201 ctaaaaataa gtacccccta ttagtatata tatttatata ttaaaaaaaa gttttaaata
     1261 aagttatatt ttttacaaca gttttgtaat taaaaacata ttgaaggtaa atttaaaggt
     1321 actatatact ataaatatgt cgacacaata aatttaaaaa ggagaactaa atatgaaaaa
     1381 aagattaata ataatgatat tgtctgttgt cttagtacta tcaagtatac ttacgatatt
     1441 tgcatattca aatattaaat ataataataa taaattgata tattctaata tgatagataa
     1501 aaaaactcaa aatagtgtaa aggaaatcct aaaagaaaat aaaattaatg aaaaagacat
     1561 agatacattt attaaagcag tcaacaatta taataaattg caagttaaaa tacttcaaaa
     1621 taatataaat atatctaaat ctggatattc ttcaatcaat gcaaaacaag ttccatataa
     1681 cctagaaaaa ttgcaagata attgggtgaa aaaattccct gattatatgg atgtaaattg
     1741 tagaataact gcttttagac tttttaaaga ttttataaat tcaaataaga aatttactgg
     1801 agactctatt gatttgaatg tcgacttaga cactataatg aataataaag atgctaaatt
     1861 tagcaccaaa gatgtagaaa aatttatcaa cttcttttct gcaataccag ctaaagatac
     1921 agatgataca atcaaaattg cagaacaaat aaaaaatgaa tggaaaaaaa gaaaaatatc
     1981 ttttaaaaat aataaaaata tatctattat aaatggattt cttcgttatc cagaaactaa
     2041 aaacgtattt ataggtcata caggcatatg tattaaaact aaaaatggca ttctattttt
     2101 agaaaaatat ggtgttacat caccttatca agttactaaa tttaaaaata aaaaaggtgt
     2161 aaaaaattat atgtttaata gattgaagat gtcagaaggt gaaattgaat tgcctgaccc
     2221 tataataatg gaaaacgata aacttatgaa ataataactt taaacgcaaa atagtattta
     2281 taattactaa gtcaatataa tagagaaggt ctatagattt gtgtaaatac aatcttatct
     2341 acaatcttat ataagaatgt ttatataaat aagatagatt aattctaagt cctattattc
     2401 tattgaattt tattattatt ttacttagat tctattgtta ttttacttag aacttattgt
     2461 tattttattt aagttctatt actattttca atcatataaa tttacaaaag actgtacata
     2521 aatttatttt atacaaactt atctacagtc ttttgtgtga atatatctaa ataatatcgt
     2581 atttatttat ttcctctgca cagtgagaat cagaacctaa aactaatgga atatcgtatt
     2641 tttttaccaa ctctaataag tatccatcta tataaggctc tttgcaatac tcttttctat
     2701 ttccagaaac attataatca agttcataac ctttttcttt aattaacttt attaagtctt
     2761 caagaattgt atttccttta taatcgtatg gaaatacctg attaaacttt ctaactaaat
     2821 ttaaatgacc tattcttttt ggtttatgta tacctaaatc tgaatttaca gcttttatca
     2881 atgtttcata atacttatta taaacagctt caatacttcc taatttgtcg attaaatttt
     2941 gaaactcctc tacactgtaa tctacacaat aataatcatc atcaatttta attatatgta
     3001 ctgataaaag agagtcttct aattcctttc catatttatt taaattctcc tttatccctt
     3061 cttcgtatcc ttctataaaa tccacttccg acccaacatt tatcttgatt ttaccctcat
     3121 acttttcttt caacttcttg acatcattaa aatactttgg taaatcttca atactcatac
     3181 aactatcatt ttcaggagca gggtctctaa atccatttgc atacggaaaa tgttctgtaa
     3241 atgtcatttc tgttattcca acatccattg ctctttgtac atatttttca aaatcatcct
     3301 tacttccatg aggacaataa ggtgaatgta tatgaccatc tttcataaat aattcactcc
     3361 ttttttaaag aaaaaatcta atataaatgc ataaattttt taacttcaaa ataatatttt
     3421 taatcatgct aatttttatt atatctaata ttagagtaat tttaaactgc cttttaagta
     3481 tcttatcttt agtactattt tcaacttaac attaatatta ttttttaact taacattaat
     3541 actatcttta aatagtaata ctcaatatat aattaatcaa ataactcaaa ttgtttgatg
     3601 aagcttttgc agtatttaaa gaatatataa ttatttaaca ttaaataatc caatcttgta
     3661 ataaaattta ctcattaaag tatattacag aaaatatctc aaaataaact ttttagttca
     3721 tgatatactc ttttttgcat gaaattagat atatttttat catttcaaca atgaaaggga
     3781 ggcttacatc catcttgaga taacccctta gataaaattt ttaataatta aacatcataa
     3841 aatataaaaa tcgcttatat caaacgtata agcgattaat tatatttaca tatgtttttt
     3901 agcctctaac aacattttat tagcttcttc catatactct atagcctttt caatactctt
     3961 tgctgtttct tcttttccaa tagctctagc cttttcaacc cactctctaa atccttcttc
     4021 atgagtttca ttgtgattta tccaatgtac taaaagtatt tttaaagttt tttcatcctt
     4081 agactctgat gattcctcat gactatgttc atggtcgtgg tcgtgagcat ggtcatgtgg
     4141 atgttcatga catatatctc catgagtatg ttcgtggcag tggtcatggt catgatgtcc
     4201 atgatgatat cttacaatct ctttatcaaa aatcattagt tattccccct gttattttat
     4261 attaaactct ttttttataa ggtttacaga atcttttaca agtagtgcta atggtttacc
     4321 ttctaatcca actaactcca cattttctgg cataataggt aaaagtatct taagtgcctc
     4381 actgtctgca atagcttcac ttatattaca agtaacttct cccatcatag aattaggtat
     4441 tattacagaa ataggtgcaa ctattatatt tgctttttta gctgatacta ctatagcatt
     4501 ttcccctgta gctcctttat tagcatgagc tttcatcata gaactagttg ctatagaatt
     4561 agtccctagt gcataaattt ctacatatgt aggtaactct tctctcaaag aagaaactat
     4621 ctgggctcca atacctccac ccattccatc tatcactgct attatcatat gaaattcccc
     4681 tattaattaa ttttcttgta ttattatctt atggtccatc aatcttattt ctttaattat
     4741 cccatctatt atcttttgct ctcctaataa atccgttaaa taaatctttc catcacaagg
     4801 gtctatatta accacatttt ccataacctt ttctaactca ttattacttt tacaaatgaa
     4861 agctgatgat tcacacatat aaatctcctc ctaatatgat ttttattaaa aatcaaaaaa
     4921 agattccctt aggtatttca atactccaaa gaaaccttca tgatttcagt ttttgtatat
     4981 tattatacct taagtatatc actttaattt atgtaaagtc aattaaagtt aacgcttttt
     5041 attatactct tcattcttgt atttttcata tagttcttta actaattgct tctcataatc
     5101 agtcaaatcg ccctccacaa actcaacatt aaattgttca gctaatccct ttactatgtt
     5161 tttctgtaga atatctatgt ctatttttct gccaatttca ttttctattc cacttgcttt
     5221 tttagctgta aatttcatag ctctttcttt caattctggt gtcttagttt ttattaattt
     5281 aaacaatttt tctacatcaa aatccagtat tattgaacca tgttgtaata aaacaccatc
     5341 ttttctgctt tgagcacttc caattacctt cttattatta atagtaactt catatgatgc
     5401 atgtgcatta aaacatgcag ctgataaatt ttccctactt attctctcgc ctctatttaa
     5461 attatctgtt tctataccac ttagattcaa tcctttaact agaccttcac ttatatatct
     5521 ataagatagg ttgatgctct tatcgattaa aggattatct tctcctataa ttatactata
     5581 agttagctca ttatcatgta atacagctct tccacctgta attcttctag tatagtctat
     5641 attcatacat ctacacttat ctaaatctat ctcatcttcc aatttctgaa aatatcctat
     5701 actcaaacaa gctggttccc atgtataaaa tctaagagtt ggcttattat gtcctttctt
     5761 atatgcagta aatatagctt cgtcaattgc catattcatt gctccttcat atgatttatt
     5821 atgtataact ctccactgat tcatgattat tacgccccca tttttagtat aaatatatcc
     5881 tagaaatcct ctatagattt atttctctta ttgctaaatc tatctcactt tgatttaaag
     5941 gtcttgaata gttgtatata gtacttccgg gtctttcatt atagtatggt ctattacaat
     6001 ccttacaacc tcttatttca aaaggatagc ctttgtttat gtcttttaaa atatcattgt
     6061 caagctttat actatttaag tatccatttt taaacttaaa atactctttt ggataacctt
     6121 tatttatcat ataagacatt aactgtaccc ttctataact ttctatgctt ggttgattta
     6181 tcttttccat tttagttcct ctaactggag taaatgcaaa taaactaatc gttacatcat
     6241 tttcctttaa ataaatatat aaattgtata tatcttcatg actttcaccc ataccaacta
     6301 ttatatgtgt acttatttta ttgggatatg attttgacat ttcagttata aattttaact
     6361 tttcatcata gttatttcct tttatttttt catatagttc tttatttgat gcatctattg
     6421 ctattcctat tttatcaact cctactgaaa aaaacttctt tatctgttca tcattttcta
     6481 attttgcaga tagtgaaatt ggcatgtcta tcttaccact tatatagttt ataaagtcta
     6541 aaacagagtt atgtgcttct tcacttgcca ttgattgaat acatattctc tttatatttt
     6601 ttcctttata agcttttaaa gcatctaata tttcttcttt tgaaaactct ggccaaatta
     6661 ctcttgataa tttatctttt ctagtactac tttctattga ttgagaacaa aaactgcatt
     6721 tatttataca tttttctcca atcattatat atgctgtggt aggaggtata tcgctttttt
     6781 tgtttaatat cccaagctct attgctgtcc caactgatag tctaatcata aaacgcaaca
     6841 ctccttacac tcatttattg tcatattcat ttctatagcc ttattctttg cagaccttga
     6901 tggtaatact attctattga ctccacacat tatagataat tggtctagct cttttctata
     6961 cactcctctt ggtctcatac aacctagatt aatttcagta tctggtaaat taattctagc
     7021 ttcacataaa atatctgcta ccccttctaa ctcaggaggt tctacatttt catactcagt
     7081 cccttttgtt ggaataagga ctataaatgt aagcttatta ggaggatttt tctgtaggta
     7141 ttctataatt tcatattcac cctttatttg tcctcctttt aaaccaatac atatatgtgg
     7201 tgctacttct gtatgttctt gtattgtatt ataaacttca atataatctt ctttactctt
     7261 tttcatctta taaacttctc ttattgtctc atcatcaaac actaaatcaa aagaaactat
     7321 atctaaatac ttacacaact ctacaatgct atctttatcc ataagaccta aatgagcatt
     7381 caatctatat cctttctctt ttaagttttt aatggtatta atatgagtat ttatagggac
     7441 atctccctca taactacaac cgccactcaa tagaaagcta gttatattcc ttgattgtac
     7501 tttttcctca tattcttcta ttggcaccat attgtttaag tagtgaccgt tgcaatgggc
     7561 acatttcaaa ctacattgat tgcttgtagt actaagagct agtgtctggt taggataagc
     7621 aaactctatt ttatttccaa aattctttaa tttaatatca aatgctattt tcattttttc
     7681 agatagattc ttattcatag tttattcctt cttaagtaca ttttactctt ctagtaattt
     7741 ctttgctgtt gggaactgct ctatattcgt atgtggtaag aaacatgcag aaataaattc
     7801 atccatataa cttggatata cactaagttc aacataagtt atttgattac ctatttcttc
     7861 aagtttattt tttgcatctt tacttatgag agctaagtaa gaaccaacaa gtgaactatt
     7921 ccctatataa gtaaattttt ctctatcaat gtcaggaagt aaccctataa ttattgagtt
     7981 ttctatgtct aggttgtttc ctataccacc tgctatataa accctatcta atatactaaa
     8041 atccatacct aaactatcta caagagtgta tgcacctgaa tatatagctc ctttagctct
     8101 gatgaaatta tctatatcaa cttcattgac tactatatca ttttctaaat caaattcttc
     8161 tttaaatgct aatacatact ctcctatctc atgctcatta aatctaactc tcttattatc
     8221 taaatccctg tatattttac ctcttctatc tattacacct ttagtaatca tttgacagat
     8281 taaatcaatt ataccagaac cacatatacc aactggtgca cattcatcaa taatctttaa
     8341 tgttggctct aaagtttctt tatcaataat tactttctct attgctcctg ctgatgccct
     8401 cattccacag cttatccctc caccttcaaa agcaggtcct gctgaacatg cacagctcat
     8461 catataatct ttatttccaa atacaatctc tccattagtt cctaaatcta taaataaaac
     8521 attttcttca cttgaccata tccctgctga taatacacct gctgttatat ctcctcctac
     8581 ataacttgca acagatggag ctaagtacac atatgcactg tcatttacaa aaagaccaac
     8641 attttcaccc ataagttttg gagattttaa aaatggtggt atgtaaggtt cttgtcttaa
     8701 gaagtctgta tacacaccca agaataaact tgtcatagtt gtattcccgg cagccactaa
     8761 tgtaactaca tcttccttat ctattccatt tctttcatat attgttttta atagtggatt
     8821 tatagtttcc tcaactatag ctttatgaag tgtctctaat ccatttttct tagttgaata
     8881 tataattcta tttataacat ctgctccata ttttatctgt gcatttccag aagatgcttt
     8941 atctactacc tcttttgaat ataggtctac taaacaaaca actactgaag ttgtaccaat
     9001 atctattgca actccgtata aggaattttc tttattacct tgttctatat ttatgattgt
     9061 aagtttcttt tgtttttgaa catatgttat tgtaacctta aaatcactct ttctaaatac
     9121 agttggcatt tttctaagta tatccaatct aaaatctatt tcattatatc ctaggctatt
     9181 tctaacatat ctttccaatc tatctacatc acttatatta tcatctaaat ttggttcttc
     9241 catttctatg tatttctttt ttatatttgt tttaaattga aggttatgtt cttcaattat
     9301 ttttttagct ctttcaaaaa tttccctatc ctctttttta ttactacctt ctatcttcat
     9361 accatgcata gaagatgata ctttagaagg aacttcaatt tctatatctg atattacctt
     9421 tgtacaacaa gctagtatat aaccttgctc ccactcatca tctgttatat gtcttgtttt
     9481 ttctgtatca acttttccat tcaataattt tactttacac ttcccacaag acacgtttcc
     9541 attacaagga gcatctatga atatatctgc atttcttgca acctctagta atatatcacc
     9601 ctcattacaa taaacctctt tattgtttgg tgtaaaactc acttttatca tgttatccct
     9661 cccaatttgt tctatatatg ataaaaggaa agtatagcta aatttgcgtg cactcaaatt
     9721 ttactatact ttccccaatt ataataatat cttaatcttt aattattcta atccagcctc
     9781 gtattcttta tcacttaata acgtatcaag ttcagccaca tcagctagtt ttacttttaa
     9841 tatccagttt tcatatgggt cttcatttat acattctggc tcatcttcta atttttcatt
     9901 aacttccaat acctcaccat taacaggaga tattaaatca gaagctactt tagaagactc
     9961 aactactcca aaatctactc ccttagttac agtatcttct acttctggtg tttcaacaaa
    10021 caatatttct cctaattgat cttgagcata gtctgttata cctatatata caacatctcc
    10081 atcaattact tttacccact catgatcttt agagtatttt aattctggta ataatttcat
    10141 ttcgatagac ccccaatatt atattattga ttaaattact taaatcatat taatattata
    10201 tcatattatt ttaaattttc catcaattac attacacttt ccatagctaa tgctggatga
    10261 cctttttctt ctaagaaagc taatactcct tcagaatcgt cagctatagt ttcatcacaa
    10321 accatatcac agaagttttc tattccagtc atttctttaa ctgtagcatt taatttatca
    10381 gctacataat cttttaattc ttttggcatc caaactattc tttctggtcc accttctgca
    10441 taagcgaatt ttttagaaga tatgaaatgt cttccgtgtc ccataaatcc tggagtttga
    10501 actcctccac ctgtcataga agcaagttct ccgaaagtca taccaactgg agtaacactt
    10561 gcaaattctc tgttaactac aacaaatccg tttgcttctg gcattatacc acagatacat
    10621 tcgaagcatc cacaagaagt cattgggtct tctagtatag aatataatgt aacagattca
    10681 actgcacctt gagatatttg gtttacagtt tcatttactg aattccatac acctgttcta
    10741 tcatctaagc actcgccttt ttctattggt tgacaaggac ctgttgggtc taattcttta
    10801 gtagccttag catctaacca gctaactgct ccacaaagac caagtctttc aggagtaaca
    10861 acacaaacgt gagctggtgc aaatgattga cataagttac aagaatagaa agtatcaaca
    10921 ctttcatcaa ctaatgaagc taatctttca tctctagcat tgtacttagc tattgcttct
    10981 ccttttaatt cagacacttt ttcagcatct gtaattatag ttatttcaca cttatcaact
    11041 actgattcaa attcatctaa catcttagca tataaaactt ctcctatatg ctcaagtcta
    11101 aatcctttat caaatgcatc tttagagatt cttacccaag ccatatctct ttgtccaacg
    11161 tgcattacac cttctatata gttcaagaag tagtggaaac gtctttcaag aactggctcg
    11221 aaatcttctt gcatattttt accagctatt ttaactatta cagcaagcgg taatcttaat
    11281 acaccatctg catcaacttc atctatattt ggtcctatta tttctatctt atggtcttca
    11341 acttctgttg cttctttctt aacaacaagc tcccaagctt cagttctgtt tccaccaaac
    11401 tcagcaaaca tatcgctctt tctaattctc tcaccttcaa atgctgctgc aaatgaaact
    11461 gggataggta tttcagtaac ttttattttt attcctctag cttctaatga agtagctact
    11521 atcttatcgt aatctttttg agttaatagg ttcattggaa cttcaagaac tgtttggtca
    11581 gttataactg ggaatcctaa agctattgct ccagcacctg cagatacaac taattcactt
    11641 aatggtccaa atgcattaac aaatgcagga actctattag ctgtgtactc taataatccg
    11701 tttaaatcac caggagttaa tccaccgaat attaatgctg ctcttactgc aacagataca
    11761 acgtgtataa cagaagttac atcatatcct agaggtataa ctcttagctc aagacccatt
    11821 tttactccag cttctatagc ttggtctata actttaccaa ctaagaaagt tagtaaacct
    11881 ttagattggt aatcttttat aacttttgct gctgattctg catctggaca ctcaccaaga
    11941 acaactgcaa caccaggtat atctcctgta actaatggta caccaagtga tctgattata
    12001 gggtcagtta tatgacctgc acatggctca ctatatggag catccatagt tgaatatttt
    12061 aatgcttcaa ttacttctgc agctaaagcc gtagctaaac cagcattaaa agcatgttct
    12121 agtaaatgag ttctatttat taaagatttt actacttcta aagcaccttc taagtcccct
    12181 aaagtgttca tcttttgtcc tgttgcagca tatatacaag gtaatgaata tgctgtatca
    12241 gggaaagcaa ctttatgctc ttttccattc ttttctatag cttcagctaa catagcttga
    12301 gccgcaccta aagcttgttc tgaccctgta aagattatat tatatagatt cattctattt
    12361 ccccctttaa tatttacacc tattttttat taactatacc ctaaaaaccc tagaaagcaa
    12421 atccaccttc atttaactct aagaaagagt ctgcatataa agatgccccg ttgattatgt
    12481 cacaagattt tacagttttc attaattctg ggtcacatgg attcattata gctgaactaa
    12541 atccattagc cattgccatt gctaagaatg cactatctaa aacaggtcta acatgcttag
    12601 gacatccatt agatacgtta gataatcctc ctgtagttaa aagtcccatc tcagtcatca
    12661 tttttatagc ttctaaaact tctacttgtt tttcttgcat accttttata actaagcata
    12721 atgggtcaaa tagtatatca gttggctcca ttcctaacat taagcctttt tctaatattt
    12781 cttggcagta agccatacgc tcatcattat ctcttgggat accttctttt gcacataaac
    12841 ctataaccat tgcttcgtat tcagctgcta tatctattaa atcaaatctt gagccagcat
    12901 cagcagagtt tattattggt tttgcattag ttctatcata aactttaagt ccagcttcga
    12961 tagctttttt attagctgta tctaatgcta taggaacatt gttgaactca gattgaagta
    13021 atttgattcc ccatgtcatt atttcttcgc catctctttc ggcaggtcct atattaaaat
    13081 ctatatagtg tgctcctgct tctaattgct cttttgctct ttttaagatt ggagctgggt
    13141 ctctttctgc taatgctttt cttattgaag gtgatataca atgtattctc tcacctataa
    13201 tcataaattt ttccatttat aatgtccccc tttaaattta atattaatag ttagttaata
    13261 ttttattagt ttgtttggca tgctttttct tggtatcctt ttaagaactt aggtaattcc
    13321 atagattcct ctggtcctat aactacattc catcctggta agttatcttc tatatcacct
    13381 ttaagtactg caacttttcc tggaatgata aggtctttac aattagttac ttcttctacc
    13441 ttactttctt taatgaaagc agagattgag tttccaccaa atttaccagc agcccaagat
    13501 gtaagaactg aatatccacc tgcatctggt attactaacc atactggaac ttttgatctt
    13561 tctatatcac cagcaactat gaagtaagtt aatgcaaagt caacagtaac taatactgga
    13621 gaattttcat ctgggttgtt gattggataa atctttggtt caactctcat tggtctttgt
    13681 gggtcagtgt atatgttttg tcttagtgca aataatggta atgccttagc ataactaata
    13741 tcatctatta ctattataga accgtatttt atagtgaata ttgatgataa agcaacttcc
    13801 atcatagggt ttgaattaga taatctattt gcaaatacta ttgaaggata tccaaatgtt
    13861 ctatcttgct cttttaaagc tgttcttcta acttgtatag cattagtgta agtatctttt
    13921 atgttttctc ctgttacatc taatactaat tctttatatc cagcggcttg aattaattca
    13981 actgtttcat ataattcttc taagctacca gcttttacac ctaatggtaa acttgctcct
    14041 ttaactactt ctatcatatc tttgtagttc tctttagttg ctccatatac cattggtcta
    14101 gcatctttta atacttcaac agcttcttta actacttgtg catcatcaca agctaaaata
    14161 taagctactt ctaatccact gccttttatt ttatctatta attttaaata cgcatcttta
    14221 tctccacaat actctaaaac agccatttca actttcattt gttcaccaat tcttacatag
    14281 tcaacttttt tcatgttagc tattttagca tctactgctt catcactcat acaagtacag
    14341 aatgaaactg catatctatt tctacttact aatgtttttt catgtctaaa taatactgtt
    14401 tctccaccta gttcatactc agatgcacca gcaccaactt ttaaagcttt cattaaaggt
    14461 gcagtagcct ctgataattt tgctatagcg tcatcagaca tatgtgggca tttacctact
    14521 tctacagctc ctgaagcaac cttcatagag aaagccatac aagttgggaa tccacaatcc
    14581 ttacagtttt tctttggtgt taatttaaat atatctaaag cttttaatgc cattattttt
    14641 tccccctaac cttaacaatt taattaagct aatgcgttaa ccaattcttt tatagtttct
    14701 attgattttg gatgacgaag tataactgcg tttgaaccac ctactaaaac acttgctgca
    14761 gttgaaactt ccattgctat agttctttct tctggacaac cccaatctgg ctcatcttct
    14821 attggagcta tagcttcttt aacatgacct acttcaaaag ctactggtgt tatgataggc
    14881 atttgtaatg tcttatcatt ttgaccaaat gctgcaagtc taattctatc catagtagat
    14941 gcaacgtact catatccata accaactgct gaacatccaa cattcataac tatattttca
    15001 cctttaacgc ctagttgagt taacaatacg tttaattgtt tagctaggtt tatatcaaca
    15061 gaagattcag ctcctacttt atgtgcataa gccataccag ctgatgctcc tactcctttg
    15121 tagttatctt ctactgctga cataaataag cagttatgtc catccaatgt ttgagctaat
    15181 ttttcaaata atttaccatc tttttcatga tttcctgaac cagctacaac taaaggtaat
    15241 tttattgctt caattactgc cttagctaca tcagcacatt catcaactga cttatctaat
    15301 ccatttgggt cagaaccatc aaattttaaa caaataaaat ctgcttgtgt attagcttca
    15361 acatatttag cccactctac tggacaatta gctacatctt tgtataattc cttatatgaa
    15421 tcagtccagc tttcaggata aacgtctgat atttgtatac ctatttttgg agaattacct
    15481 acttctccat caaaactata aaaaggtaat acattttccc cacctaattt aattgctttt
    15541 tcccctattc ctacttcaac ttctgatatt tttccagaat atttttgagt agacatttta
    15601 aatgccatat ttttttcccc cttaagccta ttttaattct aatttagcaa aaatttcttt
    15661 catagccact ttagattttg aatcttcagg taaattaact aaaggaatac ctgtagaatc
    15721 atattcatat atcaattcat ccataggtac aacaccgatt agctctaggt tatgcttttc
    15781 aatttcttct ttaacaccat cattcataac cccatttgga actttattta caatcaaaag
    15841 tattcttcca acacttaact ttagttcctc agctaaatct cttattctag caacagcttg
    15901 tatgctacgt cttgagcaat cactaaccaa taaaagtgta tcaacatgtc tagtgacttt
    15961 tctacttaga tgttccatac cagcttcgtt gtccatgact aagtatttgt aatgaccaga
    16021 tattttgttt acttgttctc taagtatccc atttacaaaa cagtaacatc cttcaccttc
    16081 tgacctaccc attactaata agtcatatcc ttcgccttct tcgattatag aatttaatct
    16141 aaattgaaga tattgtgcct ttgtcatacc accagggaat gcattgccta acttttctct
    16201 ttgatttact tcttctctta tttctcctat tgttgcttca acttcgatac ctaacacttc
    16261 atttatgtta gcattggcat cagcatctac aactaatact ggtccctttt tgtccttaac
    16321 caaataatca attaaaagcc ctgtgagact tgttttacca gttccacctt ttcctgcaac
    16381 agctatattg tatcccattt taagacttcc cccttaaatc tagccacaga gccttatgta
    16441 ctctgtctgt ctattataat ttaggtacag aatgaacaca ttctccatgt acatcatgaa
    16501 gtgcttccat tagcccttca gataaacttg gatgtggatg tattgcatcc cctacttgtt
    16561 ctactgttaa tcctagatga acagcaagtg acaactctgt caataaatct gtagcatgag
    16621 gtcctactac tgctgcacct attatcttat cagtttcttt gtcagctata acttttacaa
    16681 atccttggaa atgtcctata gcttgtgctt ttccaagacc tctaaaatca aattgaccta
    16741 cattgtattc tacaccttca gcttcaagtt gcttttcagt cttaccaaca ccagcaactt
    16801 caggttcagt ataaacacat cttggaattg ctctataatc tactacctta gtcttaccta
    16861 aagcattttc aactgctacg ataccttctt tagaagcaac atgtgctagg aaaggagtat
    16921 ctattatatc gcctattgcg tatatacctt ctacattagt ttcaagatgt tcattaacta
    16981 ctacttttcc tctttccatt tcaattccta tatcttctac tccagaattg tcaaggttag
    17041 gtcttctacc aacacatact aatgcatatt gtgcttctat aacctttcca ttagaaagag
    17101 ttgcaaccgc cttaccatca actacttcac aagtttgtac tccaatacct gtaataacct
    17161 taattttatc tttcttaaat tgtctaagta gttgtttagc tacatctttg tcttcattta
    17221 atagtatttg gtcaaccatc tcaactatag ttacttctgt tcctaaggct ctaaagaact
    17281 gccctatctc acatcctata actccaccac ctactattaa catagactca ggtatctctt
    17341 caagtccaag aacttcatca ctagttatta caacttttcc atcatatggg aacattcttg
    17401 gtacaactgg aacagaacca tttgctagta ttatcttatc tgcttttata gtttctactg
    17461 ttccatcatc tttagttact tctatagtgt ttttatcaat taattttcca aaaccattaa
    17521 ctaagtttac tcctcttttt tcaaataaga actctatacc acttattaat tggtttacaa
    17581 ctttattttt tctttccatt atggcagtga agtttggctt aactgtacca tctatttcta
    17641 taccaaaatc ctttgcttct tttacagtat tcaatactcc tgaagaagca agaagtgctt
    17701 tagttggtat acatcctgca tttaagcaag ttcctccaac tcttcttttt tcaataaccg
    17761 ttacatctgc accaagcata gaagctttta tagctgctac atatcctcct ggtccaccac
    17821 ctactactac tatcttcatc cttaattccc ccaaacctaa tataatctac aatcttgcaa
    17881 tactataaac ctgcggcatc cagtatagca ggtatattct cttctgcccc aattgccatt
    17941 atatgaacac catcacataa atcttcttct tttaattgtt tgataagttc ccctgccatt
    18001 tttattcctt cacttaccca gttttctttt ccagctgctc ttaatctttc gatttgttca
    18061 tctggaacga atattcctgg tacatttgca gtcataaatt tagccattcc tggtgatttt
    18121 aaaggtacta ttcctgctaa taccttgcaa tccatatccc ttgttaattc tctaaacttt
    18181 ctcattgtat ttatgtcata aagtgcttgt gtttggaaaa atttagcccc tgcttttatc
    18241 ttcttttgca tttttaataa ttgaacttct attggagaat attctggagt tactgatgct
    18301 cccagataaa aatctggaga accttttaac ttattaccag ccatgtccgt acctgccatt
    18361 aaagtctcag cagtttgaag tatccccaca gaatctaaat caaacactcc cttagcctgt
    18421 ggatggtctc ctacgctagt atggtctcct gttagtgcca acaaattgtt tatcccaaat
    18481 acacctgctg acaacatttc tccttgtata gctattctat ttctatctct ccctgttatt
    18541 tgaataactg gctctaatcc agcatctttt aataatttac atgtagcaag agaagttgct
    18601 ctcataactg ccgattgaaa gtctgttaca tttgctgcat gaactcttcc aactaacggc
    18661 tttgcacatt ctattaaatg tgaaaggtca gtcccttttg gaggagccat ttcagtagta
    18721 actgcaaatt ttccactttc taatgtttct cttaataagc tcatctctaa tccccctttt
    18781 aaccttctaa aaaaaacttt tatgaatttg ctgctgcttc tttctttttc tttgcactta
    18841 aacttcttgg attattttgc ttagaatagt cttttggtgg tcttatttct gctaagttat
    18901 ctaattgtcc tatagcttct agtctttcat atatctttat ccaagcacaa tcattctctg
    18961 agtttacttc acacttacca tttttagctc caccacaagc cccattcata agacctttag
    19021 cacacatagt tactggacat atcccacctg tccaaccaag ttcacagtct ccacaagcct
    19081 tacaggcttc ttcatattct ccaactcttt gtacttcccc tataaacaaa gtattgttgg
    19141 ctggataaac tggcttatct tttaaattct taactattgt ctgtgtacca tctccacatg
    19201 ctaaagataa aactgcatca gcttccttag tctcttcttt tagagccttt aaatcctttt
    19261 tagactttaa aaggttacaa gcagggtcaa gcattacata tcctaagatt tttttgcctt
    19321 caccttcaag agtttccttc atttttaaaa cttcttcttc cccaccactt ttacaagttg
    19381 ctgcacattg attacaacca actaaaacca gcttgtcaaa atcctttaag taccctagca
    19441 cttcttctaa tggtttattt tcagaaatta tcatatctac ttccccccta aaccttccac
    19501 atatatatct ctatttattg ttttgtaatt tacaagcctt aactacatgt tttgcaagta
    19561 tagatgttgt aactgaacca acccccgctg gaactggagt tatcattgat actttgtcta
    19621 aaactgcatt tgtatcaaca tctccacata aatttccttc ttcatcaaca tttataccaa
    19681 cgtctataac taccgctcca tctttaacaa aactttcatc aaccatctta gctctaccaa
    19741 tagctgctat tagcacgtct gcctctgctg ctacacctga tagattttta gtttttgaat
    19801 gacatattgt aacagttgca tgttcactta ataaaagcat tgatactggc tttccaacta
    19861 ccattgatct tccaagaact gttaccttac ttccttttaa atcaacatta tagtgtttta
    19921 atatctcaac tactgctgta ggtgtacatg gtggaaatcc acttttatcc ccttccatta
    19981 ccttagcaga gtttattgga ctaaaacaat ctacgtcttt ttccggtgca ataacatatt
    20041 ttataacttc ttcatttaag tgttttggaa gaggtctaaa acataatata ccattaacat
    20101 ttttgtcatc attaactctc tttaatacat caatatattc ctcttgtgtt atatcctcag
    20161 ctaactctaa cacttctgtt gttataccta tgttttgaca tctctttagt gctcctcttt
    20221 cataagataa atcatcactt cttgccccaa ctctaactat tgttagttta ggatttatac
    20281 cttctttaac tagtaaatcg acttctttta ttaattcttc actaatttta tcagctacag
    20341 gttttccttt tataatttgt cctttagttg acattccttc catacattca caactcccct
    20401 acaagcaaac ttaaattaat ttattctata taccacatat taataacata gtatctactt
    20461 tcgtcattta aaaattattt tccaagagct ttttcaactt tggcataaac ttcatcacaa
    20521 atttttacac catcttcaac taacttatct acttctattt ttaccttatt aacatattct
    20581 ttatcttgta tagaatttat atttataact acatttaatt gtccaccaag tatagcagct
    20641 cttaaacact gaactcctac accaacatca cttatagcaa gtcttgaacc tttatcaact
    20701 aaatcttcat gtaacttaat agcttcatag caaactctta ctatgtttaa tggtacttca
    20761 caagcaactt ttaatgcctt ttccattgtt tctgctttta ttcttttttc ttcttctgtt
    20821 tctttaggaa gcccgtaagc ttttgataat ggtaagaaac attctgcatc atcatctatc
    20881 atttttaata aatctttttc taactttcct gccttactta atatctcttt tacgctttct
    20941 tcatactcag catacttctt cttccctatt gtaaggttac ataccatact acctaaagcc
    21001 atacctattg cacctactag tgcagcagct cccccaccac ctggtactgc ttctttggaa
    21061 gatagtactt ctacaaaatc aacacaagtt ttatctgcta ttttcatgat aatcctcctt
    21121 gaaaggatgt aggaaaatat ctcaaattat tcgagatatt ttacgaacta ttttaagata
    21181 tctcctactc tttatttata aattaatttc cttagaataa tccagctatt actccatttt
    21241 catcaacatc tattttttct gctgctggaa cttttggtaa tcctggcatc ttcattattt
    21301 ctccagtaag tgcaactata aatccagctc ctgctgaaat tgttgcttgt cttacagtta
    21361 ttctaaatcc tgttggtcta cctaatttag tttggtcatc agttaaagaa tattgagtct
    21421 tagccataca tactggaact ttagtaaatc caagtttttc taagttagct atttccttat
    21481 cagcttcagg agtataatct acaccatctg ctccataaat cttagtagct atagcattta
    21541 tcttgtcttt tatagataaa tcatcttcat agcagaatct aaagttattt tcttcttcat
    21601 ccaataatct taaaacttct ttagctactg caattccacc ttctccacct tttgcccata
    21661 cttcagaaag agcaacatta acacctaatt ccttacactt gtcttcaaca agtttaagtt
    21721 cagcttcagt atcaagtggg aatctattga ttgcaacaac tgctggtaat ccatatactt
    21781 gagttatgtt ttcaacatgt tttaataagt ttggaagtcc tttttcaaga gcttctaggt
    21841 tttcgttatt taagtctgct ttagcaactc caccattgta ttttaatgct cttacagtag
    21901 caactattat aacagcatct ggttttaagt ttgccattct acattttata tctaagaatt
    21961 tttctgctcc aaggtcagca ccgaaaccag cctcagttat tacatagtct gcaaagtgca
    22021 tagccattct tgtagctata acagagttac atccatgagc tatatttgcg aatggtccac
    22081 cgtgtacaaa tgatggtgtt ccttcaagag tttgaactag gtttggtttt aaagcatctt
    22141 ttaataatgc agccatagca ccattagctt ttaattgtct agctgttaca ggttcacctt
    22201 catagctgta tccaactatt atgtttccta atctttcttt taaatcagat atatcacttg
    22261 ccaaacagaa cgctgccatt atttcagaag caacagttat atcgaatcca tcttgtctaa
    22321 ctgctccatc acctttttta cccataccat caacgatatt tctaagttgt ctatcgttca
    22381 tgtccacaca tcttctccaa gttattttct tagggtctat tctaagtgca tttccttggt
    22441 gaatatggtt atcaagcata gcagctaaaa gattgttagc agctcctata gcgtgaaaat
    22501 ctcctgtgaa gtgtaagttt atatcttcca taggaacaac ttgtgcatat cctccaccag
    22561 ctgctccacc tttaacacca aatactggtc ctagagatgg ttctcttaaa gcaactaata
    22621 catttttgtc tagtttagca aatgcatcag caacacctat agtagtagta gtttttcctt
    22681 cacctgctgg agttggattt atagctgtag ttagtattaa cttagctttt ttaccagtct
    22741 ctctttttag tagattgtag tcaacttttg ctttgtattt accataaagt tctacatcat
    22801 cttcacctag tcctaatttt tttgcaacct ctctaatatc ttgaggctta gcttcttgag
    22861 ctatttcaat gtcagattta aatcccataa ctgaatctcc tcccacaacc taaatagatt
    22921 taaaatataa tttttgtaaa aattgtatta atatgtaaaa attatatttt ataagataat
    22981 tatacatgta aaattactta aaaactagct actaagctat taaaatattc aaacttaata
    23041 gctagttatt tatctaatta cttacttatt acctatctat tgcctatgtc taacctttaa
    23101 ccaacttaaa atcccaaatc aaaatttcta tctattttat ttatccttaa gtgacattaa
    23161 tttatctttt attgctttaa tctgttcttt cgttgtttca ctagaatcat atggagattg
    23221 attagctcta tcagaatcaa taacatcttg gttatagtaa ataaatccta aagattctcc
    23281 atcttctaag ttttggatta taaattcttc atcttcctta tttctaattt tatttcctac
    23341 tacaaatact ttatttacac ctatatcatg tccaagtttc ttaacttttc tataagtctg
    23401 tagacttctc tctcctggtt ctacaactac tataaaagca tctacacctt gggctgttcc
    23461 tcttcctaag tgttcaattc ccgcttccat atccataacc actacatctt tattttgtag
    23521 tattaaatgt gagcagagtc tcttcaaaag aacatgctct ggacatacac atccagtacc
    23581 accagaatct acggttccca atgtaagtaa tcttactcca ttgtattctt tacagaaatt
    23641 ttcagggata tcgtctactt taggattcat cttaaacatt tttccaaatg aacctacaga
    23701 agcagctgtt ctatctgaaa ctaatttttt catttctgat ataggtacta ttgattcata
    23761 tacttctttt ggaaaaccta gtgctaaagc taaattagca tcagggtcgg catcaacagc
    23821 aacaactctg tatccgtctt cagcaaacat cctagagagc attgaagaaa aagtcgtttt
    23881 acctactcca cctttacctg ttattgctat cttcataatc cctaccacct tttattacta
    23941 tttgcttttt agtagttaat tttacagtta aatatttttt atactctata tttttataca
    24001 ctaaaaataa actacagcta gtccttacag gaaccaatta aatttaccct actagctttt
    24061 caaagcagca tgtatagttt tattattctt taatattgtt tatattttat ttattcagta
    24121 tattcattat atttcgatgt tctttacttt aatatttgac tttattcaac tttactcaac
    24181 cagaaacttc ttataaaaac gtttattcta taacttcact attttttaag cttcagcctt
    24241 ttctgccatt ttttcttcaa ctaatgcttc aaagtgaaca cgttttttct ctatatcttc
    24301 tattattgta gctgctaact cttttggatt tgtatttaca gtaaatgtag ctccaacttt
    24361 atcttttaac tcactagtta atatatccac tgctcttgaa gaaccagcta ttggaggcat
    24421 tattcctaaa taagtgtcta taccagaagc tacaacatag catcctatag caactgcttt
    24481 ttctgacatc cattctggag ctatacctac aactggaagg tcacacatat ccatatctaa
    24541 gtaattagct gctgctccaa ctaaatctag tatacgactt atatcaacac aagaacccat
    24601 atgtagtact ggtgggattc ctactaattt acagacagtt gcaagacctt tacctgcata
    24661 tttttcagcc gcatcagatt ccattaaacc aaacttagct gctgctgaag ctccacaacc
    24721 tgtagtaact actattatgt cgtttttgat taattctttc attattgtta cgtgagcctc
    24781 atttgaaact cctttagcat tgttacatcc aactacacca gctgctcctc ttaatactcc
    24841 agcttttaga cagtctgtta atggcttaac tgttcctgct gggtctatat gagagtttac
    24901 aactctgtct aattgattta ctactgcttc tacactatac ccaactgttg cgcctgattt
    24961 taattcaggt ataagtactt ttgatttatc tctattttta aagtttaaaa tagcttcttt
    25021 aactattgct ttagcatcat caagtgcttg ttcttctcta aattcaatat aagttgaacc
    25081 tgttatctta gcttttggag aagttgttac aaatttagta tggtaacaat ctgcaactct
    25141 agctaatgca gggaatatac attgaacgtc aactattaca gcttcaactg ctccagttat
    25201 tattgcaagt tcttgttgat ggaagtctcc tgctattttt acaccatgtc tcattgtaat
    25261 ctcattacct gtacaacaca tacctgctaa gtttattcca tcagcaccaa cttctttagc
    25321 taaagctact aagtctggct cttctgatgc taaaactatc atttctgata atgctggctc
    25381 atgtccatgt aatattatgt taactttatt ttcttctaat actgctaaat tagcctctgt
    25441 tcttcttggc actggagttc caaataagat atcgcttaga cgagtaccta tcatagaacc
    25501 accccaacca tcagccatag aagttctaag actcatgtgt attaaactat ctatatcacc
    25561 agtacatcct atatgagtag agtgcattat tgttgcaatt tctctatcta tagctcttgg
    25621 ctctatagcg tattctttcc atattttttg tcttggttct ggtgcatttt ttataaatct
    25681 taatgtccca aaaggttttc caaattccat taatgcaatt tctgccactt catgtgctac
    25741 atcatagata tctctacctt ctgtctctac atcccattct ttagctaaag taattaactt
    25801 agcttcgtct cttattttat agtttccatt tggatcagct aatgctaatg tatgtgctat
    25861 atctctagca tggtctgaat gggcagctgt cccaccagca accattcttg caaagtttct
    25921 acctactata gtatgctcat ctgctccaca taaccccctt ggagtcttag ggcttattct
    25981 acatggtccc attccacata ttctacagca aacaccttga agaccaaaac cacattgtgc
    26041 cttaaagcct tcttttctat catacattgt ttctacgcct tcttctctag ctttagctat
    26101 catcttctgg ctgtttaaat caattgttaa cattttttca tccatattcc tatccccttt
    26161 ttttgatttt taaaatcccc caggtgactg acttcatgtc ataaaataat aaaataaatg
    26221 ctttctaagt tgattatata ctaattttca gtgctttgct ttagatttaa atattttttt
    26281 aacaaaatta gataatacat aaaaatttta ttttctacac ttaatttaag ttgattttat
    26341 cgcttgtttt tggctatttg ttaatatcta aacctacttt tttgatactt cagtttaatt
    26401 aaattatgtt ttgatttttt taaatcaaat ttgaaatttt tctagtcatt tttatattat
    26461 tgcagattgt ttgtgtattt attatctttc actactgttt ggacttatta cagattttta
    26521 ctccctaaac ttaattagta aaaacttctt agaatagcat tttttggact aatttttttg
    26581 aattttttta tttttcctac tttaatatta attatagctt caaattgaac atttgtatat
    26641 attcatttga agctataatt attttttata taaaactttt ttaatacagc attaatttag
    26701 acattttgac tattttttct actttaattc atttatttca agcgatttat cctatttcat
    26761 atttttagct acaatagccc catatgcaat agaatataat ttagattcat gtgaatcagc
    26821 tcttgacact aaaactatag gagcttttgc acccataatt atacctgctg atttagagtc
    26881 tgccatatat gtaaaggctt tccctattcc attgccaacc tctattgttg gaaccaatat
    26941 gatatctaca tctccagata cttcactttt aaaccctttt atctcactag cttcttttga
    27001 aacagctaaa tcaaaagcca gaggaccttc tacaattaca ttttcaccaa attctccttc
    27061 tttacaagct tttgctaaca tatctgcatc tacagttgct tgcatcttag gatttacttt
    27121 ctctttagct gccaaacatg caacttttac agtttccatt cctaaagctt ttgcagcttt
    27181 tatagaattt tttaatatct ttgccttttg ttcataatca ggagctatat tcatacctcc
    27241 atctgtagtt ataagtaatt tatggtattt ttctaactca tataccataa catgacttaa
    27301 taaactatca gttctaagac catagtcttt atttaacact tcttttaata tgacagatgt
    27361 atctaataat cctttcatta caaagtcggc ttctttactt gatactaatt taactgctat
    27421 ttctgcacac tctttgatat catccttatc cactattttg ataccactta aatcaaatcc
    27481 tatttctttt gatatttctc ttattttgcc ttcatttcca accaatatag gagtaattat
    27541 atttctttct acagcatcct ttattgccat taaaacttct ttatcatgag ctgctgctac
    27601 agagagtaca attttctctc ctcccttaag ttgttctaaa atatcatcta atttttttat
    27661 caaaagtaac ccctccctta tatatattta actaaataat accatatttg ctataccttt
    27721 tctcaattta ttttttatgt tataaagaat ttatatgact aaaagacagt ttaatatttt
    27781 attctgggat ttcatcaata gctcttaaaa gctttgcagg aacaccacct acaatagtat
    27841 tttttgttac aactgctcca gcagcgataa ttgcattatc tccaatagta actcctggaa
    27901 ctcttcatac aaaaaagagg cttatctcta ttttgagata gccccactta accgataaaa
    27961 ttaaatacaa cctaacaata tccttactta tttattccaa gttctcttag acaagcaata
    28021 gtaatttcat cttgtggatt taattcataa gctctttcta tatagtaagt tgcatcatca
    28081 atatcaccat tatttaaata agccattcct aaattgcaaa gtatttctgg gtcttccttt
    28141 atcttagccg cttgttcaaa atactctatt gctttatcta aattattctt ataagcctca
    28201 caaagcccca attccactat agtatccacc tgattaggct ttattatcaa tatcttctca
    28261 agatacatct tagcctgctc tatttcgccc atacccttat atccaagtgc tatcataaac
    28321 agcaagttcc accaatccat atgttcttct tctaatggaa gtaacttctc taatccttcc
    28381 ttaaacttac cttggaatac aagtgtatat ccttcttcat attgaacttt aaagtccatc
    28441 ttgcctagat tctcttgtac ctcagctact aaatcagcat ctaaccctag ccttaatgct
    28501 tcttcccaag tcagcttagt ctttaaatat tgaccttgat tataataatg gtagcctaag
    28561 tgataataac ctaatgcaaa attctcatca acatcaatta ccttttcaag cttagccatg
    28621 gcttcaagta agaatgcatt cattgcctta tcatccatat ctttttgata ctgtttagct
    28681 atctcttggc aaacaatcgc atagttatac attgcatcta aatcatctgg atacattgtt
    28741 ataaatgctt ttatatatat taaagaatct gtatgttcac caatctcaaa gtattttctc
    28801 gacatatatc ccatatacga tttcaaatcc aaatcaatat cttttgataa agcatttaaa
    28861 aacttctcat actctgcatt gtttttaaac ttactatcta ttccaattat atatatcatt
    28921 gcatctgcta tagacattga atttaactta tcttgagcag tcttttcttt tatccctttt
    28981 actaatacct cattttttat aggtacatct aatccgcctt ttggcacttt atagcttttt
    29041 aatttaaact ctccaccatg ttttatagtt ataaaagcta attcttctgc tttttttagc
    29101 aaatattttt ctatcttaaa atccatttta tcctcctaaa tacttaaatc tctactttat
    29161 gagttttaga tttctcaaat atttttttat catcatactc aaataagact acattttcta
    29221 cttctcttat ttctgtattt atatcatttt ttaaatccac aacattaaat ctaaatggct
    29281 caaaatgaac atattttatt atatgtttag ctggtttatc aacaaaacta ggtaaatatg
    29341 ttgataagtt atcattatct tgtaaaaata catggcatct attcttaaaa tcatccacat
    29401 ctagcttatt aaaaaactgt ggtatatttg atgttttacc cagtgatata tagtcatatt
    29461 ttaatatatc attgaataca tcattatcta tgttgatttt ttcattatag aaatccatca
    29521 atattttata taactgattc tttccttgtg ccaaatcaaa gtagccattt tcatcaaaat
    29581 acttggcaaa aacctcaaag aatttaaaag gactttgttt ataaaatctg cctataatat
    29641 atctcataga taacacaaag tttttagaat tgtagtatct ctccagtata tcttctatat
    29701 cctttagctt caaagtttct tcatagctta tataattatt gtataaaaac tcatatggag
    29761 cataatcctt atatttaaaa ccatgctcat ctgcaacttt tctcatacct gtccctttaa
    29821 tcatctttaa aaagcctaat tgcagatgct ctatacctag attaaataca tcattaaaag
    29881 aattttcaaa gctcttataa tcttcataag gcagtcctgc aattaagtct aagtgctgat
    29941 gtatatttct ataagatgct acagtttgta caacatgaga caacttttta aaatcatccc
    30001 ttctacctac tgcatcaagt gttttttcat tcgttgactg aacccctatt tcaaattgaa
    30061 aaagaccttc tttacaatca gccaaaaact caagcatctt atcatctatc aaatacgctg
    30121 taacttcaaa atgataagtt gtatatccat tatcattttt cataaggaaa tccatgattt
    30181 ccatagccac ttttctattt gcattgaatg tcctatctat aaactttatc tgtgatactc
    30241 ttgcatctat taaagctttt aaatcactct ttatcctgtc taaactaaaa taccttagac
    30301 ctggaatagt agatgataaa cagtactggc agttaaaagg acaacctctt gatgattcat
    30361 aatacactat tctattttca tattcttttg gattcaagtt ttcataagga ctaggaatct
    30421 catccaaatc ttgaagtaat tccataggct tattcactat tatctcattg ccttgtctat
    30481 atacaagtcc atttacatca tttattttca tctttccttg caagtgaagt actaagtctc
    30541 taaaaattaa ttctccctct ccataaagta tataatccac aaaatcatac tttttcattg
    30601 cctcataact atcataactc acttccggtc cgcccaaagc tattttagta ttaggtctaa
    30661 ttttttttag attatcacaa agtttaacta tatctcccac attccagata tatgttgaga
    30721 ataatattat atcatattca tttttatata tatcctttaa aatatagtcc aaatcgttgt
    30781 ttatcgtgta ttctttcata ttaacttcta tcaaatctct tacaaattct tttagatatc
    30841 ttatagccaa atttgtatga ataaacttgg aatttaatgt tgttaataaa attttcaaat
    30901 atttctcacc tctttttaag attatactgt ctttttgtac cccttatagg ttaaaatagt
    30961 atatgtaaag tctaaataat atctatataa ttaatttatt aatttcaagt aatgtgagga
    31021 aaggaagttt tttatggaat taattgaaaa agtatctatc aactctttat caaaaagaga
    31081 tttactactt attataaagt ctcttgaata cactaatgaa aatactcaaa ttagtgattt
    31141 cattgacctt aggaataata tagtaaagga attatgtttt ttaactgaca ctaaggaaaa
    31201 agatttttta gattatctag aaaaaaatag cacactctaa taacctaaac ctttttaaac
    31261 tttaatatat aaatacctga tttagaattt atttttatgc ttaatatatt atcattaaag
    31321 gttttcgaca taactgttat ttgattaaat atagggtcta ttatagtatt accttcttca
    31381 agtcttataa gacttcttgg gtctatgctt ttattatatg ccttaactcc aattttatat
    31441 tttctcaatt taacaacata ttcggacata gaaaaaataa aatctatatt atcttcattt
    31501 aatttactat cttcacccaa aatggataat atagattttt ttatgctatt aattccttca
    31561 ttttctacag ttctatcttc catatataca tcatatagaa catttaaaac atttaataaa
    31621 atactttctt catcataatc ttcagatttc aaatcaatgt acttatcaat taattgtatt
    31681 gctttaatcc ttgccatctg aaactttaca acatctgatt tgtctaattc tatagaaaat
    31741 cctattatct tgtctttaat actctgtagt aattcgacat actctatttt actattattt
    31801 ataagattgt gcataatgga attatacact acagaaccta ataaataatc aaataaaagt
    31861 tcttcttttt taaaataaat aacatacttt tttattagtt taataacctc atcttcgctt
    31921 ttttgaatat tagtatttga taaaattagt ggtagtaaaa tatttaaaaa attgatattt
    31981 cctatttttt ctccataaaa tctatagtaa ataagttcat ctctcagtct attttcgtct
    32041 atatacacac atctttgcgt tttatcatct attaaggatt ttgtatttat tatagttaaa
    32101 tctttaagag ccctacttgc actactaaaa gaagatgtgt agactttttt tagtaattcg
    32161 tcattcataa atatatcctt tctgaaaaat tgaatgtaaa taaattgtta ttaaaaaaaa
    32221 agagacagcc cgtttaagaa gctgtcatat atagggggat agtagtgtac aaaaatcatt
    32281 gtaatctact atctatatat tattatacga tactactttt ttatatgtaa agcatcagtt
    32341 ttcgacatcc ctatatttta cagttataca aattgcgtac taagttcaat tttattcttt
    32401 ttatttcaac attttatcat gctacattct ttccggagcg tgcattccca acaatttcag
    32461 agaattttct aaagtttgtt ttgttgctaa aactaatgca agtcttgctt ttcttatttc
    32521 caaattttct actaaaattg gattgtcatg ataaaattta ttaaacgctt gagctaaatc
    32581 taacataaat ctagtcacta tatgtggctc atttttcttt agagcaagta aaatattctt
    32641 attgaatgat tctataactc taagaacttc tgcactatct tcatcagcta atagactata
    32701 atctatatca gaagtcactt ctacttctgc ttttcttaaa actgcacagc atctagcatg
    32761 tgtatactga acatatggtc cagtctcacc atcaaaacta agcgttcttt cccaagagaa
    32821 ggtatagtct ttaattctac tgttagataa ctcttggaat actactgccc caacacctac
    32881 ttgcttagat atttcatcca catttttagc atttggattt ttagctagta taatttcctt
    32941 agttttatct atagcttgat ttaaagcatc ttctaaaaat actactcttc cttttctagt
    33001 cgacatagta ccttcttcaa gtgcaaccat accaaatcca acatgaatta aatcttttgc
    33061 ccaatcatat cccataagct caattacctt aaaccattgt tggaaatgta agttttgttg
    33121 agaaccaact acatatatac acttgtcaaa atcataagtc tcttttctat atattgcagc
    33181 agctaaatct cttgtcatat ataacgttga accatcattc ttagtaataa gtgctggtgg
    33241 cattttatac tcttctaaat caacaattct tgctccttta gactctttta aaagattttt
    33301 ctcttctaat agttcgataa ctctgttcat tttatcagaa taaaaactct ctcctgctaa
    33361 agaatcaaat tctatatcta aaagtttata cactctgtta aactctttta aactctcatt
    33421 tctaaaccat tgccataatt cttgtgcttc ttcgtcacca ttttctaatt tattaaacca
    33481 tgctcttgct tcatcttcca tttctgggtg ttgttcagct tcatcatgga atcttacgta
    33541 taattttagt aattctggaa ttggattaga ctctactact tctttttctc cccacttttt
    33601 aaaggccact attaacttac caaactgagt tccatagtca cctaaatgat ttattcttat
    33661 agtcttgtat ccttgcgaat catatatctt atataaagca ttacctataa ctgtagttct
    33721 tatgtgccct atatggaaag gttttgctat atttggagaa gaatattcaa ctattacagt
    33781 tttatctttt ccaaaatcac tatgtccgta attttctttt tcatctaaaa cttttttaat
    33841 tacagtttct gctaattgag atttatttac aaagaaattt acatatccgc ctaattgtat
    33901 tacttttgtt atctctccac ttggttctat actttccgcc aattcacttg ctatcatgtt
    33961 tggagctttt ctaaacactt tggctaattt aaagcatgga aaagcatagt cccccatatc
    34021 cttatttggt ggaacttcta taagtgcctc tatctcttct ttcgataaat cttctatttt
    34081 ttctttaagg caatttgata ttgccacctt aaaatcttgc attctgttgc tcctctctac
    34141 attgttcctg gtttatattg tgcttatctc tttatgttaa atcaaatttc gtgttttttc
    34201 aagtatttca ttacagtgta aaacacttta ataattatgt tttctgtata tattaaaaat
    34261 actataattg cagtttttaa tcacactatt attgattttt tataagactt ttttctattt
    34321 tattgtttct tgtactatcc agttgacttt atatactttt tttaatcttt tatcaatttt
    34381 catacatcaa tattaatttt atatacattt atttt
//
LOCUS       DAGQWR010000024        33412 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.024, whole genome shotgun sequence.
ACCESSION   DAGQWR010000024 DAGQWR010000000
VERSION     DAGQWR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.024"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            207..347
                     /locus_tag="MIW95_001026"
     CDS             207..347
                     /locus_tag="MIW95_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150371.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(522..842)
                     /locus_tag="MIW95_001027"
     CDS             complement(522..842)
                     /locus_tag="MIW95_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBY3150372.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(962..1699)
                     /locus_tag="MIW95_001028"
     CDS             complement(962..1699)
                     /locus_tag="MIW95_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBY3150373.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1914..3737
                     /gene="acd"
                     /locus_tag="MIW95_001029"
     CDS             1914..3737
                     /gene="acd"
                     /locus_tag="MIW95_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBY3150374.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYIS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3952..8250
                     /locus_tag="MIW95_001030"
     CDS             3952..8250
                     /locus_tag="MIW95_001030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBY3150375.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEILPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8454..8915
                     /locus_tag="MIW95_001031"
     CDS             8454..8915
                     /locus_tag="MIW95_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBY3150376.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8945..10099
                     /gene="nusA"
                     /locus_tag="MIW95_001032"
     CDS             8945..10099
                     /gene="nusA"
                     /locus_tag="MIW95_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBY3150377.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVKSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELGTSEDNFSEEE"
     gene            10106..10387
                     /locus_tag="MIW95_001033"
     CDS             10106..10387
                     /locus_tag="MIW95_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBY3150378.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10377..10688
                     /locus_tag="MIW95_001034"
     CDS             10377..10688
                     /locus_tag="MIW95_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBY3150379.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10710..12650
                     /gene="infB"
                     /locus_tag="MIW95_001035"
     CDS             10710..12650
                     /gene="infB"
                     /locus_tag="MIW95_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBY3150380.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12693..13073
                     /gene="rbfA"
                     /locus_tag="MIW95_001036"
     CDS             12693..13073
                     /gene="rbfA"
                     /locus_tag="MIW95_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBY3150381.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDNLIKRVNEKNAQQNEDENYDE"
     gene            13054..14037
                     /locus_tag="MIW95_001037"
     CDS             13054..14037
                     /locus_tag="MIW95_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBY3150382.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNVGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEGVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQTKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSDYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            14050..14955
                     /gene="truB"
                     /locus_tag="MIW95_001038"
     CDS             14050..14955
                     /gene="truB"
                     /locus_tag="MIW95_001038"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBY3150383.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEVVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            15086..16015
                     /locus_tag="MIW95_001039"
     CDS             15086..16015
                     /locus_tag="MIW95_001039"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBY3150384.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEYAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16119..16376
                     /gene="rpsO"
                     /locus_tag="MIW95_001040"
     CDS             16119..16376
                     /gene="rpsO"
                     /locus_tag="MIW95_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBY3150385.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16616..17461
                     /locus_tag="MIW95_001041"
     CDS             16616..17461
                     /locus_tag="MIW95_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBY3150386.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17586..19697
                     /gene="pnp"
                     /locus_tag="MIW95_001042"
     CDS             17586..19697
                     /gene="pnp"
                     /locus_tag="MIW95_001042"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBY3150387.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19963..20706
                     /locus_tag="MIW95_001043"
     CDS             19963..20706
                     /locus_tag="MIW95_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY3150388.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            20864..22111
                     /locus_tag="MIW95_001044"
     CDS             20864..22111
                     /locus_tag="MIW95_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBY3150389.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAICLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22146..22391
                     /locus_tag="MIW95_001045"
     CDS             22146..22391
                     /locus_tag="MIW95_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBY3150390.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22401..23624
                     /gene="dapG"
                     /locus_tag="MIW95_001046"
     CDS             22401..23624
                     /gene="dapG"
                     /locus_tag="MIW95_001046"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBY3150391.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEENIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23737..24471
                     /locus_tag="MIW95_001047"
     CDS             23737..24471
                     /locus_tag="MIW95_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBY3150392.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24594..27005
                     /locus_tag="MIW95_001048"
     CDS             24594..27005
                     /locus_tag="MIW95_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBY3150393.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKSNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKSSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            27008..28066
                     /gene="mnmH"
                     /locus_tag="MIW95_001049"
     CDS             27008..28066
                     /gene="mnmH"
                     /locus_tag="MIW95_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBY3150394.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESVFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            28060..29394
                     /gene="rimO"
                     /locus_tag="MIW95_001050"
     CDS             28060..29394
                     /gene="rimO"
                     /locus_tag="MIW95_001050"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBY3150395.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFSELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29381..29923
                     /gene="pgsA"
                     /locus_tag="MIW95_001051"
     CDS             29381..29923
                     /gene="pgsA"
                     /locus_tag="MIW95_001051"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBY3150396.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            30211..31257
                     /gene="recA"
                     /locus_tag="MIW95_001052"
     CDS             30211..31257
                     /gene="recA"
                     /locus_tag="MIW95_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBY3150397.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            31433..32974
                     /gene="rny"
                     /locus_tag="MIW95_001053"
     CDS             31433..32974
                     /gene="rny"
                     /locus_tag="MIW95_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBY3150398.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT        13541 a         3535 c         6364 g         9972 t
ORIGIN      
        1 atttaatata taaaaaagat aataatagtt agacttttta tatgaatttt aataggtaaa
       61 atatcctatt gttttgtaga tatatcctaa ttatgtcaaa aaataaaaaa atttttattt
      121 attagagttt ttggaaataa taataacaga tattataatt aattaacagt ttttgtaagt
      181 atttgacaat agagagggtg taacaaatgg gaataataga gtcagcatca aaattggctg
      241 agatggttca tttattggct gttgaaaagg ggataactga tatagaagca tgggatgaag
      301 ctgtgaaaga atacagcaaa atttatgaag aaagaagaaa tgaatagaat gataaaaaat
      361 gaaaacttga tgataaaaaa taactttatt taaagaaatt caatttttat agaaatatta
      421 aaaataatat aaataaaaag atggtatttc aaatgtaagt aaaaaatcat tttgggatac
      481 catccttatt atttatttta ataatatata agtaataaat cttacttatc catatttttt
      541 atgtaacctc ttttttctag cttttttata tgctccttac gtatttcatc aagattaata
      601 ccatattgtt cttctagctt aaacatcata gtaacacatg tctgagctac atccattagt
      661 tccttagcag ttttctttat aatatcaact tcatctaaat cattattttc tccacttaga
      721 tttctgaatt tacctataca ttcagctaat tcaccttgtt cttcacccat tttgataaat
      781 gtagattcta gtgttgggtc taaattatta agctctggta aagatatagt ttttagtttc
      841 ataaaagctc ctttcaagtt attcgtattt atactaaaac atcataacaa aaataatact
      901 aaaaaaaaac tttaaattca acataaaaac ttacatatat aataaaaatc gtgaaataaa
      961 tctatatttg tcttaataca gcctcatata aaacttctcc aataggttta tttattacaa
     1021 gagaagctga cttatcatag actgtatttg ctttattaat aagagcaata taatcacctt
     1081 tgtaataatc tataaaactt gctgctgggt acacagctaa tgatgtacct cctataatca
     1141 ataaatcagc attacttata gcagaaattg ttttagtaat gacatcactg tcaagagctt
     1201 cctcataaag tactacatct ggttttacta tagaaccaca gttgtcacaa taaggaatat
     1261 tacctcccag atttaacata gattctaagt caaaaaactt accacattta gtacaataat
     1321 ttctatgtac agaaccatga agttctagca catttttact tccagccatt tgatgaagtc
     1381 catcaatatt ttgagttatg acagctttaa gcttacccat ttcctcgagt tttgctaaag
     1441 ctatatgagc attgttgggt tttgcattag gatatattaa tttatcctta taaaattcaa
     1501 aaaattcttc tggatatcta acaaaaaaag tatgtgaaac taattgttca gcagtaaatt
     1561 gtttattgag tttttggcta aataatccag tagaacttct aaagtctgga atattagatt
     1621 cagttgaaac acctgctcca ccaaaaaata caatattatt gtgattagct attaagtctt
     1681 ttaagctatt tgcatccata gttacacctc caaattatta tatataaatt gtactacata
     1741 ttaggtcttt tatgttttat taatttaaat aaatatgtag tttaataagt taaataaagg
     1801 gaatataaat aaaatgtata cagagtataa atagacaata tttactaaaa taacaagttg
     1861 tcgtataatt atattaaaat tgttttagtg tataagaagg gcgggggaag tacgtgaaga
     1921 aaaaagcagc tttagcaaca ttggctatgt tacccttagg tgtggtaaat gcacatgctg
     1981 atggagatat aggtatagtg actataaatt atttaaatgt aagaaatgag ccaactgccg
     2041 aaagtagcat agcctttgtt gctaaaaaag atgataaagt tttgattaaa gactcttcta
     2101 atggatggta taaaataaaa gctgaatctg gacaagaagg ttgggcttca tcaaaatata
     2161 tagcaaaatc aaatagtgac tctttaagaa cgtctacaaa taaagaaaaa caagttattt
     2221 caaatagttt aaatatgaga aatggagcag gtacaagtta cagagtaata actgtactaa
     2281 aaaaaggtca aaaagtagaa gtaatatcag agagtaatgg atggtctaaa atcaaatatg
     2341 atggaagact aggatatgta tctagctctt atttaggaga cgtttcaaat tcaactaata
     2401 aatcaaaaac taaacaagtg aatacaactt cactaaatgt aaggagtgga ccaaatacaa
     2461 gttatggttt gttaggcaag ttgccaaaag gaagtaaagt agaagtaata tcagaaagta
     2521 atggatggtc caaaataaag tataatggaa aagatgccta tatatctagt atgtacttat
     2581 cggatgtaag tcaaagtaat tcagacaatt ctagtcaaag caatgataaa aagaatactg
     2641 ataaggttgt aaatacagct tctttaaatg taagaagtgg accaggttct acatatagta
     2701 agttgggaaa agtttataaa ggaagtaaag taactgtact atcagaaagt agtggatggg
     2761 ctaagattaa ttttaacaat aaagaagcat ttgtagtagg caattattta tctacttcag
     2821 cagatacttc aaataataac tcaaatagta actctgataa cagttctaat agtaatggca
     2881 ataattcttc atcatcaggt caagtaaatg gtatgtcagg tataagtggg gctaaaattg
     2941 attataaatc tttgagttat actttagaat ctcatataag taaacaagtt gagaaagcag
     3001 catcaggagg aaatgtgata gctccaagta ataggaaaag cactccaagt cctgaattta
     3061 gtactttttc agctcaaaga acaagctcat ttgtaaatgc aagttcaagt gatatagaat
     3121 attatttaaa tcctaaaaat tttacaaata ctactaaagg tatgatgcag tttttaaaga
     3181 ttaacagtta tagagatggt atttcagaat caagtcttaa ctcgtatctt aatggtttat
     3241 cttctagtgt atttaaaaac cagggagcag cctttataaa tgctgccaag aagtataata
     3301 tagatgttgt atatctagta tctcatgcta tgtgggaaac tgcttatggt aagtctacac
     3361 ttgcccaagg tcaaacatta acatcttata agggacaacc tcttagtaag ccagttaaag
     3421 tatacaattt ttttggtata ggagcaatag ataaaagtgc taatgtttca ggtgcagaag
     3481 cagcgtattc caatggctgg actagtgtag aagctacaat agatggctct gcaaagtgga
     3541 tatcacaaaa ttacgtaaat agttctaagt acaatcagaa tactatctac aagatgaagt
     3601 ggaattatga ttatacatgg catcagtatg caactgatgt aaactgggct aatggaattt
     3661 ctggaattat ggaaaattta attggtcttt atggaggagg aagtagtttg gtttttgaag
     3721 ttcctcaata taagtaatac ataaataaaa aatatagact atgggtagtt ttcattttat
     3781 ttaaattatc tatagtcttt ttgtatataa tttataatct gtaaatttaa tgttaacaaa
     3841 acttttatgt tataatatta attctaagta aatataagtg agtaataggt agaaatagat
     3901 aaaaagtggt aataatatat ataaagataa ttttgagaaa ggaggatata tatggaaagt
     3961 attaaggaat atttggataa gttagaaatt aataatagtg gtcttggaaa gcaacttaaa
     4021 gaagtttata taaatagagt agtttatttt aaggaagata agatagttta tttttatcta
     4081 acttctaaag atattgtttc tcatgaactt ttagataaat ttaaagaaga attgatgtat
     4141 aaactcgatt attttaaaga tatgaggatg aagataagat ttactggtct tgaaagaaaa
     4201 tccaataaag atgttataaa aaaatattgg aataatatat tgtatatatt aaagtatctt
     4261 tgtccatcta ttgcagggtg gtataaacaa gtagaatttc tttgtttaga agaagaatta
     4321 aaaataaaac ttccaaaagg tatattttat gagcgattaa tgaaaaaaaa tgtaacttat
     4381 gtacttaaaa ccgtgcttag cgaagagttg ggattagatt taaatataac aatagaaaaa
     4441 gctgttgatg aaaaggtaaa taaagaaaga ctcataagaa tcaatgatag agaaatggaa
     4501 gaaaaaatta gggctttaga aattggaaaa gttaataact gtgaagaaaa tgaagaggaa
     4561 agctatgtca ttaagtctga tgtagatgaa aatttaatct atggagataa tgcaaatgct
     4621 atgattgaaa atatagttga attaaatgca tcttctggaa cagttgcagt tgttggagat
     4681 atatttgatg ttgacactaa agaacttaaa aacggaaaaa tacttatgat agcttcaatt
     4741 acagattata ctagctcaat aagctgtaaa ctttttttaa cagatacaaa taaggatgga
     4801 gtacttgaaa atgttaaaaa gggagcatac ttaaaaatca agggtgacat agtttatgat
     4861 acatatcaaa gagaaattag tatgatgatt agtgggataa gaaaagaaac tagaatagag
     4921 agagaagata aatcagagga aaaaagggta gaactccatg cacatacaca aatgtcttct
     4981 atggatgcta tttgttctgt taaaaagctt gtagagcgtg cagccaaatg gggtcattct
     5041 gctattgcta taacagacca tggtgttgta caaggatttc cagatgctat gaatgcaggt
     5101 aagtcaaata atataaaaat actttatgga gtagaagggt acttggttga agatgattct
     5161 ctcattatag aggatgcaaa tgataaagaa ctttcgcaga catttgttgt atttgacata
     5221 gagacaactg gtttttctaa tacaaatgat aaaataactg agattggtgc tgtaaaaata
     5281 gaaaacttta agattgtaga tagatttagt gagttaataa atcctgaaaa agatatttcc
     5341 tataagatac aggaattgac aggaataact aatgaattaa ttaaagacaa gccaaccatt
     5401 gaagaaattc ttcctaagtt tatggaattt gttggagata gtgtattagt agcacataat
     5461 gcagagtttg ataccggatt tatatcgcaa aaatgtaggg aacaaggctt gttatataat
     5521 aacaaaaaag tagatacact tatgttagca agagtaatgt tgccaaactt aaaaagacat
     5581 agattaaatg tcgttgcaaa agaacttggt attcctcttt taaatcacca tagagcagtg
     5641 gatgatgctg aggctacagc acttatattt aataagtttt tgcagatgct tacagaaaaa
     5701 ggtgcaaaaa ctctaagtga tgtaaacaat atacttggaa agattgatta cacaaagctt
     5761 agcacaaatc atataacctt gatagcaaaa aattctatag gtataaaaaa tttatataaa
     5821 ataatttcag atgcacatgt aaatcatttt tttagagcac caagaatcct tagaagtgta
     5881 ttaaatgaat ataaagaagg attaataatt ggttcggctt gtgaagctgg ggtggttttt
     5941 caagctgtaa agaaaaacgt gtctgatgag gaaatgaaaa aaataataga tttatacgat
     6001 tatattgaag taatgcctat agataataat agatttatga ttgataaagg agaagtaaag
     6061 gatgaagaag agttaaggga gttgaatagg aaattaattg atacagccaa aaaatttgac
     6121 aaaattccag tagctactgg ggatgttcat tttttagaca aacatgaagc tgtcttaaga
     6181 aaagttctta aatattctca aggttttaaa gtagatgaag aagaaactta tttacatttt
     6241 agaacaacag atgaaatgtt ggaagagttt agttatcttg gagaagaatt ggcatatgaa
     6301 gttgtagttg aaaatagtaa tctgattgcc gatatggttg aagatataaa acctatacca
     6361 gatgatactt atcctccaat tatagagggc tcagatgtgg agttaagaga aatgtgttac
     6421 aaaaaagcca aacgaatcta tggagaccca ataccagaaa tagttcaaaa aagactggat
     6481 agagaattaa attcaataat aagcaatggg tatgcagtta tgtatattat agctcaaaaa
     6541 ctggttgcaa aatctctttc agatggatat ttagttggtt caagaggctc agttggctca
     6601 tcttttgcag ctactatgag tgatataaca gaagttaatc ctcttccagc acattatatt
     6661 tgtgaaaacg aagactgtaa atattcattc ttttatgaaa taggtgaatg gggctcaggt
     6721 gttgatttac cagacaagga ttgtccaaag tgtggaagaa aacttgctaa aaacggtcat
     6781 gatattccat ttgaggtttt cttaggtttt gaaggtgata aggaaccaga tatagactta
     6841 aatttctcag gaaattatca gccaacaatt cataaataca cagaagaact atttggagag
     6901 gggtatgtat atagggctgg gactataggt actgtagcag aaaaaacagc ttttggatac
     6961 gcaagaaaat atgtggaaga aaataatata agtgtaccaa atgcagaagt acttaggctt
     7021 tcaaatggat gtactggagt aaaaagaacc tcaggacaac atcctggtgg agttatggtt
     7081 ataccagact ataaagatgt atacgatttt acacctatac agtatccagc aaatgatact
     7141 tcttgtggtg ttataactac tcactttgat tatcattcaa taagtggtag aatacttaaa
     7201 cttgatatac ttggacacga cggtcctacc attataagga tgcttgaaga tatgactgga
     7261 ataaatataa cagaaattcc tttagatgat aaagagacta tgtctctatt tacatctaca
     7321 gaagcattag gtgttacacc agaagaaata aactgtccta taggttgcct tgctgtacca
     7381 gagtttggaa ctaagtttgt acgacagatg ttacttgaca ccaaacccac tacatttgca
     7441 gaacttgtgc gtatatctgg tctctctcat gggacagatg tttgggtaaa taatgcacaa
     7501 gacttagtta gagaagatat agttggatta aaagatgtta tatctacacg agatgacata
     7561 atgaactatt taatatttaa aggattacct cccaaaatga gttttacaat aatggagagc
     7621 gtaagaaaag gtaaaggtct aaaaccagaa cacattgaag aaatgaaaaa aaataatgtt
     7681 ccagaatggt atataggctc atgtaaaaag attaagtaca tgttcccaaa agcacatgct
     7741 gttgcttatg taatgacatc atttagaatt gcatattgta aagttcacta tccagaagca
     7801 ttttatgcga cttattttac gacaaaagtt gaggattttg atgcagattt aattgttaaa
     7861 ggattagatg ctataaaatc aaaaataaat gaaattgagt cactgggaaa tgatgcaacc
     7921 acaaaagaaa aaggtatgtt gaccgtttta gaagttgctc ttgaaatgta tgctagagga
     7981 ataaaattat taccagttga tatatataaa tcagatgcaa ctgagtttat agttgtagga
     8041 gaaaaaacat tacttccacc aatggcagca atacaagggc ttggaggaaa tgcggctata
     8101 aacatccaaa atgaaagaaa aaatggtgaa tttatatcaa aagaagattt gagaaagaga
     8161 acaaaaatat caaaaacggt aatagaaact cttactaatc atggttcact tgagaatatg
     8221 agtgagagaa atcaattgtc attgttttaa gttagcccaa aaagttgcaa ataaaagaat
     8281 tttatgatat catagtatag aaaatagtat actttgcaga aactaagagt gagacagctg
     8341 ttctcactct tttattttaa atatattatt aaaaagttgg tttattttta ttaaatatat
     8401 tttatttaag attatataaa ctaatcattt acaattgaat aggaggaact ttaatgaaaa
     8461 aaaatttaga agctacaatt gaggagattg taactaaaat aacggatgaa catggatttg
     8521 aaatggttga tgttgaatat gtaaaagaag caggagaata ctacttaaga gtatacatag
     8581 ataaagaaga aggaatatct ttaaatgaat gtgaattagt tagtagagaa ttaagtccaa
     8641 tactagatga aaaagaccct ataaaagaaa attacttttt agaggtatct tctccaggac
     8701 ttgatagagc attaaaaaaa gacagagatt ttgttagata tcaaggtaga gatgtagatt
     8761 taaaattata taaaccatta aatggatgta agcaatttga aggtgaactt gtaggattaa
     8821 cagaagataa taatataaaa atcattgcaa atggtaagga aatggaattt aatagaaaag
     8881 atgttgctat tgtcagatta gcaataaagt tttagtggta tattaaatag ggaggatata
     8941 aaaaatgaat catgaattta tggaagcatt agatgaatta gtagttgaca gaggtataga
     9001 taaagaaata cttatagata ctatagaaca agctctttta actgcataca agaaaaactt
     9061 tggttcagca caaaatgtta gagttgaatt tgatagagac aagggagata taaaagtatt
     9121 ttctcaaaga gttgttgttg atgaatcaga tttatatgat actttcttag aaatagagtt
     9181 agcggatgct agagagataa accctaatta tgaattaggt gatattatag aacatgaagt
     9241 tacacctaaa aactttggaa gaattgcagc tcaaacagca aagcaagtag ttgttcaaag
     9301 aataagggaa gctgagagag aaatagtgta taatgaattt atagaaaaag aaaatgaaat
     9361 agttacagga gaggttgcta gagcaaataa aaatgttgtt tatgtaaatc ttggaagaat
     9421 agaaggtata atgactcaaa gtgaacaaat accatttgag aattataaag caggtcaaaa
     9481 aataaaattc tatatacttg aagttaaaaa gactaacaaa ggacctcaaa tagttgtatc
     9541 aagaagtcat ccgggtctag taaagagatt gtttgaagaa gaagttcctg aaatatatga
     9601 aggaatagtt caaataaaat ctgtagcaag agaagcaggg tcaagaacta aaatggcagt
     9661 aaaatctata gatgaaaaaa tagacccaat tggagcttgt gttggaccaa aaggacttag
     9721 agtaaaaaat atagtggatg aacttggcga tgagaaaata gatataataa aatatagtga
     9781 agacccagca gagtttatat cagcagcact tagcccatca aaagttgtta gagtagatat
     9841 agatgaagat gaaaagtctg ccttagtaat cgttccagat tatcagttat cgcttgctat
     9901 aggtaaagaa ggtcaaaatg caagactagc agcaaaattg actaactgga aaatagatat
     9961 taagagcgaa tctcaagcag aacaagaaat gttaaatctt gaaaattcca aagaaataga
    10021 agctgaggat gttgaagctg aatttatcga ccaagaagag ttaggtactt cagaggataa
    10081 ttttagtgaa gaagagtaag gagggatgat tttgaaaaaa gtaaaaaaag tgcctcaaag
    10141 aaaatgcata gcatgtcaag atagagactc taaaaaagag cttataagaa tagttaaaaa
    10201 taaagaagga aaaatatttt tagacccaac aggaagagcc aatggaagag gagcatatgt
    10261 atgtaaaagc tctgaatgtc taaaaaaagc tataaagagt aaagcattaa ataaagcttt
    10321 taaaatagat gttccagatg aagtctatga taatctactt atggagttag aagaatatga
    10381 aaaataataa agaaaaagta ctttcgttct tagggctcgc aactcgtgca gggaaaatag
    10441 tgtctggtga tgattcaact ttgcttgacc ttaaaaaagg aaaagtaaat ttgattttaa
    10501 tcgcagaaga tgcatcaaat aatactaaaa aactttttaa agataaatca acttttagaa
    10561 atattccata tctatttttt tcaacaaaag aggaaatagg atttgcaata ggaaaatctc
    10621 caagagcagt tgtaggaata aaagatgaaa atttttctaa aaagataatt gaactaatag
    10681 aaatttaaaa taaaataggg ggtgatcttg tgtcaaaaac aagagtatac caaatagcag
    10741 aagaattaaa tatatcaaat gaagagctta taaataagtt agctgaatta gatataaatg
    10801 taactgacaa agattcagta ttagaaggtg aagagttaga attagcactt gaaatgttag
    10861 gtgaagattt atctcaagaa aatggaaatg ttatagaaat agatggaaag ttgacagtac
    10921 aagtattagc aacgaaatta gataaatctc catcagaaat aattatgaag cttatgaaaa
    10981 tgggaactat ggcaacaata aatcaagaga taagttttga gattgctgct ttggctgcca
    11041 aagactatgg atttgaatta acagtagctg aaagtgatga tacagaagct ttagaaatag
    11101 aagctttaat ggaaattgaa gaagataaag aagaggattt aaaacctaga ccaccagttg
    11161 ttacagttat gggacatgtt gaccatggta agacatcttt actagatgca ataagaaaaa
    11221 ctgatgtgat ttcaggtgaa gctggaggaa taactcagca tataggtgct tcagaagtta
    11281 aaatcaatgg acacaaaata gtatttttag atactccagg acatgaggct ttcacatcaa
    11341 tgagagcaag aggagcacaa gttacagata tagcaatatt agtagttgca gcagatgatg
    11401 gtataatgcc tcaaacagtt gaagctataa accatgctaa agcagcaggt gtacctttaa
    11461 tagttgctat taataaaata gataaaccag gtgcaaaccc agataaggtt aaacaggaat
    11521 tagcagacca aggattatta gttgaagact ggggtggaga agtaatagca gttccagtat
    11581 cagctaagaa aaaagaagga atagatacac ttttagaaat ggtattattg gttgctgaaa
    11641 tggaagagtt aagagctaat ccaaataaaa gagcagtagg aactgttata gaagctgagc
    11701 ttgataaagg aagagggcca gttgcaacag tacttgttca aggtggtact ttaacagtag
    11761 gtgacccaat cgttgcaggt gttgcatgtg gtaaagttag agcaatgata aatgcaaaag
    11821 gtaagagagt aaaaacagca ggaccatcta cagcagttga gatattaggt ctatcagaag
    11881 ttccacaagg aggagaccaa tttgtcgaag ttccaactga taagattgct agaagtgtag
    11941 cagcaagaag acaacaaata gttagagatg aaatgttaaa atcaactcaa agattatcac
    12001 tagatgctct atttagtcaa atgagtgaag gttctataaa agaccttaat atagttataa
    12061 aagcagatgt acaaggttca gtacaggcag ttaaacaatc tttagaaaaa ttatcaaatg
    12121 aagaagttca agttaaagta atacatggtg gtgttggagc tgtaacagaa tcagacatat
    12181 tacttgcagc agcttctaac gcaataataa taggattcaa tgtaagacca gttccaggtg
    12241 cagaatctct aggagaaaaa gaaaatgttg atataagaac atatactata atatacaagg
    12301 caatagaaga tatacaagcg gctatgactg gtatgttaga ccctgaatat gttgatgaag
    12361 aaacaggaaa agctgaaata agagaaatat ataaaatatc tggtgttgga acagtagctg
    12421 gatgttatgt gactaatggt aagatattta gaaattgtaa agtaagatta gttagagata
    12481 gcataataat acatgaaggt gagcttgcag cacttaagag atttaaagat gatgtaaaag
    12541 aagttaattc tggatacgaa tgtggtatga gttttgtaaa ttataacgac ataaaagaag
    12601 gcgatatagt agaagcttat ataactaaag aagtagaaag aaaattataa attcgtatca
    12661 aagaaaatca gttagtaaag aaggtcgatg gtatggcatc atataataga acaagaagga
    12721 tagcagaaga aataagaaaa gtcgtaagta ctatgcttat aaatggagta aaagacccta
    12781 gaataacatc tatggtgagt gtaactgatg tagaagttac aaatgaccta agatatgctt
    12841 atgtatatgt aagtattcta ggtggagatg aagaatccac actaacaggt cttaaaagtg
    12901 ctggaggata tataagaaga gaagtaggaa aaaatataaa acttagatat attcctgaga
    12961 tagtatttaa acttgatgat tcaatagaaa aaggaatgta tatggataac cttataaaaa
    13021 gagttaatga aaaaaatgct caacaaaatg aggatgaaaa ttatgatgaa taatatggat
    13081 agtattataa actatattta tgagggtgac tattttatag ttacctctca tatcagtcca
    13141 gatggagaca atgttggttc tacactttca atgtattata cattgaagaa tttaggaaaa
    13201 aatgtatatt atgtacttga tgatgaagct ccattaaatt taaggttttt ggttgaaggt
    13261 gtaaaaatct ataagtctaa tgaatttaat atgaagaact attctttaat tgcacttgat
    13321 tgtggggata agatgagaat atgtgtgagt gatgaaatca aaaataatgc atcaaaatta
    13381 atctgtattg accatcatgc tagtaatgat tcatatggag atttaaatta tgtagatatt
    13441 gatgcatctt ctacatgtga actagtttat aatttattgg ttagatttca gcaaacaaaa
    13501 gatattaata ttataaatga agatatagca acatgtttat atacaggtct tgtaactgat
    13561 actggaaact tttcgtattc aaatgtacat gcaagtagtt ttgaaatggc aaaaaattta
    13621 cttgttttag gtgcacaaaa aaatactata atacaaaata tatatcaaag caattcttct
    13681 gattattata aattattagg agaagctcta aaaggtttgg agatatttga ttcaaaggta
    13741 tctagtatag ttttaactca agacatgatg aatagaaata atattagctt taatgatgtt
    13801 gatggaataa caacatatac aagagatata aaaggtatag aagttggaat tttatttaaa
    13861 gagaaaaaac aaaatgaaat taaagtgagt tttaggtcaa aaaattatgt tgatgtaagt
    13921 gaaatagcca agttatttgg cggtggtggt catgtaaggg ctgctggatg tactattaga
    13981 gatagtattg ataatgcaaa gaaaatggta ctagaggcag tgttaaaatc aatttaaggt
    14041 gacagtaaaa tgaataagat tataagtata ttgaaaccca caggtatgac atctcatgat
    14101 gttgtaagta gagtgagaaa gattttaaat ataaaaaaag ttggtcatac aggtactctt
    14161 gacccagatg cttcaggtgt tttaccaatc tgtataggga aagctacaaa agtatgtgaa
    14221 gttattttaa acaaagataa atcgtatata tgtgagctta ctttaggtat aagtacagat
    14281 acttatgatg cttcaggtga aattttaaaa aaagttgatg attttaaatt tagtaatgaa
    14341 gacatagaaa gagcttttga tacacaaaga ggagagataa atcaacttcc tccaatttat
    14401 tcagctttaa aggtaaatgg aaagagaatg tgtgatttag taagaagtgg tagacaatct
    14461 gaaattactt taaaaacaag aagagtaaat ataaaagaca taaaaatact aagtataaaa
    14521 ggtaataaag taatgtttta tgttgagtgt tctaaaggga cttatgtgag aagtatttgc
    14581 catgatatag gagaatatct gggttgtgga gctcatatgt cgtttttgaa tagaacatct
    14641 tctggtaaat ttgacttaga taattcaata acattagaag aattagaatt attttatgaa
    14701 aataaaactt tagacaaata cttatatgat atagattatg tccttgattc gtttaattat
    14761 gttgtattaa accctaatgc tataaaatat tatagtaatg gtggctcaat agatgataaa
    14821 aggtttttaa aaaataattt cgataaagat gatgaagttg taagagtata cagtacagat
    14881 aatttcttag gattaggaaa gttatcaaaa cacaataata caataagtgt taaaagtgat
    14941 aagatgttca tataaccaat gtatttaaaa aaacaacaaa agagatagta ctataataac
    15001 ttctatagtc tatctctttt acttatgcta taatagtttg tgaataagtt aagagtaaat
    15061 tatattttag cggggaacat ttattatggt gattataaaa tctataaaag aaatagcaaa
    15121 cataaaagaa agtgtcatta ccataggaaa ttttgatggt gtacacaaag gacatcaagt
    15181 tcttatagga aaaacagttg aatatgcaaa aaaagaaaat atacagagta ttgtatttac
    15241 atttgcaaat catcctgtaa attactttag accaaactca acaaaaaata ttataagcaa
    15301 tgatgataaa ataaaattat tagaagattt gggaatagat atagttgtaa tcataccatt
    15361 tgatgagtat atgactaaaa tacctgcaaa ggatttcgta gatgagatac tagtaaaaaa
    15421 attaaaagct aaaaagatag taattggaca tgactttaca tttgcaaggg caaaagaagg
    15481 caatgttaat ctattaaaaa gcttagaaca tgaatttggt tttgaggtag aagtaataaa
    15541 acccatcaaa atcaatgatg taagagtgag tagtacttat attagaagtt tagtatccca
    15601 gggtgatatg gctaatgtca aggaatattt gggtagaaat tacaagttag aaggttgtgt
    15661 tatacattct aaacaccttg gaagaactat aggattccca acggcaaata ttgaccttaa
    15721 gaataatatg ctcgttccaa agagaggtat ttatgcttct attgtacata ttggagatga
    15781 gacttatttt ggtgctacaa atgtaggcta taacccaaca gttaatggaa aaactttgtc
    15841 tattgaaaca aatattttag agtttgatag agatatttat ggggaaaata taattgttga
    15901 atttttggaa agaataagag atgaaagaaa gtttaattca atagatgact taaaaaatca
    15961 attgtataat gatacaaatt atgtgtatga aaactatgtt tgcaaaaaca agtaatgtat
    16021 gataaaatga ttaaggaaaa taaccattac tcggcatttg aatactccaa tcaatgctta
    16081 gtaataggcg tttctaaaaa aatacggagg taatctaaat gataaacaaa tcagaaataa
    16141 taaaagaata tggaagagct gaaggggata ctggttctcc agaagtacaa atagctttat
    16201 taacagctag aataaacgag ttaaatggac atttaaaaac tcataagaaa gaccaccatt
    16261 caagaagagg actattaaaa atggttggta gaagaagaaa cttattagct tacttaaagg
    16321 aaaaagatct tgaaggatat agagcattaa tagctaaatt aggattaaga aagtaatttc
    16381 attctttata aaaaagcagg tatagtgtat acctgctttt ttatagttaa ttcattataa
    16441 agtaaaatag ttaataataa aagtagttta tatgttataa aaaattataa aatgaataat
    16501 attgggtaag aatattaaaa tagtatacaa atacataatc aatttaaaag gaaagacagg
    16561 ataaatatag aaattttata acaggataat tagttggaat aaggaggata ttattttgaa
    16621 aataaagtta gtatgcgata gtttgtgcga tattccagat gaaatatcag aaaaagatta
    16681 tatagagata gttccactta cagttatttt taatgatagg gaatatatag aaggagtaga
    16741 tataaagaaa gaagagtttt ataaaaaagt aaaagaaatc aagcaaatac ctaaaacttc
    16801 acaagctaca tatatggagt ttaaagaagt gtttgataag ttcataacag aaggttatca
    16861 tataatctgt atgacagggg ctgcaaacgc atcaggaact tttcaaagtg ctatgatagc
    16921 taaaaatgat gtaaatgaaa atgaaaaaat acatatattt gatactagaa atttatctct
    16981 aggtagtgga caatatgtta taaaggcatg tgaacttctt gaagaaggac tgggatttga
    17041 agaaattata gatgaattag aaaatactcg cagtagtgta agattacttt ttgcaccata
    17101 tacacttgat tttttaaagc agagtggaag agttccagtt gcaactgctt tgataggaaa
    17161 tatgttaaat ataaagccta tatttttctt tgataatggg gaagctaagt tggtaaataa
    17221 ggttagagga ataaagaata tagcaagtaa actggtcgat ataattttag aaatgaatga
    17281 agggtcttta gaaggaaaaa tcgtcacaat aggttgtgga gataatttac acgattgtga
    17341 aatattaaaa gatgaagtaa ataaaagaat aaaagcaaga agagtattat ttactcgagg
    17401 aggagtttct atttgctctc atacagggcc agatatattg gctataagtt gttctaatta
    17461 gtagtaatat aaggtttttg catatatatg tgaagataat tttatattgt taatttatag
    17521 tatttttgat aatatagtaa atagagtgat atacgtgttt tttaataaga ggaggacaag
    17581 cattaatgtt tgaacataaa atatttaaaa tggattttgc aggaagagaa ctttctgtag
    17641 aaataggaaa gatttgtgaa atggctagtg gtagttgtat agttcgatac agtgacagta
    17701 tggtaatggt aaatacaact aaatcagcca aacctagaga cggaatagac ttttttcctt
    17761 taagtgtgga ttatgaagaa aaattatact ctgtaggtaa gatacctgga ggttttttaa
    17821 agagagaagg gaagccttca gaaaaagcta tacttacatc aagattgata gatagaccaa
    17881 taagaccttt attcccaaaa ggatttagaa atgatgttca ggtagttgca acagttctat
    17941 cagtagacca agattgtaca ccagatatag tagcaatgat aggttcttca atagcccttt
    18001 ctatatcaga cataccattt aatggaccaa caggttcagt atgtgttggc cttgtagatg
    18061 gtgcatttgt tgtaaatcca aacgcagagc aaagagaaaa aagttcaatg catctagttg
    18121 tttcaggtac aaaggaagca ataatgatgg ttgaagccgg agctgatgaa gttccagatg
    18181 aggtaatgct tgatgctatt ttatttgctc accaagaaat aaaaaaaata gttgagttta
    18241 tagaaggtat agttgcagaa gttggaaaag aaaaaatgcc tgttgaatta taccatgcag
    18301 gagaagaaat aactcaattg gttcgcgaat ttgcaactga taagatgaaa aaagcagttc
    18361 agacttttga aaagttagag agaatggaaa atatggatag agttaaagaa gaaactttag
    18421 ctcactttga agaaacttta gaagattttg aagattttgt tggagatata gaagaagtat
    18481 tacaagatat aataaaagaa gaagtaagaa aacttatagt acatgaaaat gtaagaccag
    18541 ataatagaaa acttgaagaa ataagaccta tatggtgtga aactggcatg atacctagag
    18601 ctcatggttc agccatattt actagaggac aaactcaagt acttaatgtt gctactttag
    18661 gtgctttagg agatgttcaa aaattagatg ggttagatga agaagaaaac aagagatata
    18721 tgcatcatta taatttcccc gcatatagtg ttggagaagc tagaccatca agaggaccag
    18781 gaagaagaga aataggacat ggtgctttag cagaaagagc tttattacca gtaataccat
    18841 ctcaagaaga atttccttat gctattagat tagtttctga agtattaagt tcaaatggtt
    18901 ctacatctca agcaagtgta tgtggctcaa cattatcttt acttgatgca ggtgtaccta
    18961 taaaagatat ggttgcaggt atagcaatgg gacttataaa gcatgatggt aaggttgcag
    19021 tgctttcaga tatacaaggt atggaagacc atttaggcga tatggacttt aaagtagctg
    19081 gaacagagta tggtataaca gcaatacaaa tggatataaa aattgatggt atagataaag
    19141 aaattctaca acgtgcttta aaacaagcta aagagggtag aatccatata ctaggagaga
    19201 tgagaaaaac tatatctcag ccaaaaccag aactttcacc atatgcacct aagattgtta
    19261 aaatgcaaat aaatcctgac aagataaaag atgtaatagg gcctggtgga aagataataa
    19321 ctaagataat agacgaaact ggagttaaga tagatattga acaaactggg gaagtattta
    19381 tatcaggtat tgagattgat atgattaaga aagctcaaga acttataaat aatatagtag
    19441 ttgaaccaga agtaggaaaa acttataaag gtaaggtaag tagaataatg aactttggag
    19501 catttgttga aatacttcca ggaaaagaag gattacttca tatatctcat atagctcatg
    19561 aaagagttgc caaagttgaa gatgtattga atatagggga tgaagttgaa gttaaagtta
    19621 cagagattga tgaaaaagga agagttaatt tatcaagaaa agttctttta ccaaaaccag
    19681 aacataaaaa taaataactt attatataat taaaagcaaa gtcttaggat tttgcttttt
    19741 taaatacaaa aaagtagata attttaaaat gatagttatg gatggcttat agttcttttt
    19801 ctgtaaggct ttactcttat aaagaagcta tttaaaagct gattaataga aagctttatt
    19861 gatatatata gaatcactta agttatattt agtgcataaa cagagcttat ttttaatagt
    19921 attaaataag aaacaatttg tacataatta gggggagttt taatgataat gatggtactt
    19981 aataagagta agctcaaaaa aatcataata ctaattttag tagtgttatt agtattatca
    20041 ggaggggtaa tggtattaaa gttatttaac aacaagccag tatttaattt atttaatggt
    20101 gtagatttac catatgaaag aggtacaaag gataaaagtg gatatgttgc attaacctgt
    20161 aatatagacc ttggatggga aacagagtac gtagaatcca ttttagaaac acttaaaaaa
    20221 gaaaatgtaa agattacatt taatgtaact ggaaaatggg cagaaaagaa taaggatgaa
    20281 ttattaaaga taaaaaaaca aggacatgaa ataggtaatc atggatataa acatttagat
    20341 tattctacat tatcatatga agacaattat gagcaaatag agacctctaa aaagataatt
    20401 gaagaaatta taggtgaaaa aactaaattt tttcaagcac cagcaggttc ctttggtcca
    20461 gaaacagtaa aggctgctaa agcattaggg tatacttcta tcaaatggga tgccgatact
    20521 atagattgga aatacaaaga ccaaccagag gtaatcattg atagaatgaa gaaaaaagac
    20581 attaaagata gtagtataat cttaatgcat ccaaccaacg caacgacaaa atgtatagat
    20641 gatattatag ctattgtaag agaaaaagga ctaaaaccag ggaaacttag tgatgtattt
    20701 aagtagacaa agtataaata tttggttaag attgtataag ttgtagttga attaattaaa
    20761 agactgactt ataatgtaat acatataaaa aataaatata attaaaacat agtgttttca
    20821 ataatccaat atgaaacaaa gaataaattg ttaggagaat gtaatgtata agacaaaaat
    20881 actggaaaat gggcttacaa taattggtga agaaatacca tatctaaagt caataacatt
    20941 aggtatttgg ataaatgcag gctctaggat agaagaagca caggtgagtg gaacttctca
    21001 ttttatagag catatgatgt ttaaaggaac caaaaacaga acatcaaaag agattgctag
    21061 ttcaatagat aatttgggtg gtcaaataaa tgcatttaca agtaaagaat gtacatgtta
    21121 ttatgtaaaa ctaatagatg agcatataga tacgggaatt gatgtattga gtgatatgat
    21181 actaaattct aaatttgata aaaatgatat agataaggaa agacttataa tattggaaga
    21241 acttaagatg tatgaagact ctccagatga cttatcttat gatttattag tggaaaatat
    21301 atatgctaat gatggattag gaatgaatat aattgggaca aaagaatctc tatacaatat
    21361 aactagagaa tccatgttag aatacttaaa taagtattat attccaaata acgctgttat
    21421 ttctatagct ggaaacttta actttgatga tatggtggag aaaatcaaat caaaatttgg
    21481 acactgggaa aagaaaaatt tgagtataga cataagtgag gctaagttta atccttgctt
    21541 tatatcaaaa aataaagata ctgaacaggt gaacttagct atctgcctaa aaggtattcc
    21601 ttttgaaaat gatgaagaag tctactcaat ggcagtggta aataatattt tcggtggaag
    21661 tataagctca aggctttttc agaaaataag ggaagaaaaa ggtttagtat attcaatata
    21721 ctcatcacag acattatata ggaaatgtgg tgaattaggc atatttgcaa gtatgagtac
    21781 agaaaattta caagatgtat ataatttaat aaagaaagaa attgaaaata taagagaaaa
    21841 ctatcttaca gaaaaagaaa tatctgaaag caaggaacag ttaaaaggaa attatatatt
    21901 ggatttagaa agtactagca gtagaatgat gtctactggt aaatctatgt tacttagcaa
    21961 aaaagtaaaa actacagatg aaatacttga atgtataaat aatgttaata ttaattcaat
    22021 aaagaaggtt gttgataagg tattcaatat cgaaaatatt ggtacatgta tagttggaag
    22081 agatgtagaa aagattctac acttggatta atattgaaaa aagtaaatct ctggaggtat
    22141 aaaatatgaa tttatctgaa atagcaggta aggaaatagt taacttggtc acaggagaaa
    22201 gattgggggt cattggagaa tgtgacctta tactagatga aactacaggc ggtatattag
    22261 cgcttctaat tccaaaggaa aaaggatttt ttgccagaag aaaagataag tcatttttag
    22321 aagtaccatg gagaaatgta aaaaaaatag gtaatgacat gataattata gaatatgagg
    22381 gatatattta ggaggaaaaa atgagtattt tagtgcaaaa gtttggtggg acatcagtag
    22441 aatcatatga aaaaatgaat gaagtatgta agatagtaaa agcatacaaa aaaaatgatg
    22501 aagaattaca attagtatta gtagtttcag caatgggaag aaaaggagct ccatatgcta
    22561 cagatacact tataaatttg tgtagtgcag taaatgatga gccaagtaaa agagaacttg
    22621 atttaataat gtcttgtgga gaaataatat caggaacaat tcttgctaat ttattgaatg
    22681 cacaggggat agattctgtt ttctttactg gtcagcaagc aggtataatt acaagtgatg
    22741 aatattctaa tgcaaagata aagtatataa atcctaaaaa ggtaaaaaga gctttagatg
    22801 atggtaaggt tgtagttata gctggattcc agggtgtaac agatgatgga gaaataacta
    22861 ctcttggaag aggaggaagc gatacatcag cagttgctat tggtaaagct ttagaatgtg
    22921 aaacagttga aatttacact gatgtggatg gtataatgac tgcagaccct agagtagaac
    22981 caaacgctaa agtattgagt tttatagatt atgaggaagt attccaaatg gctgacaaag
    23041 gtgcaaaagt aatacatcca agagcagtac agttggctaa aagtggaaat ataactttag
    23101 ccataaagaa tacaatgaat ccaacttttg aaggtacaaa aataggttct ttatgtagac
    23161 acttagaaga gaatatagaa tatgaacaag aaagagattt taaggttgct gttgcaaata
    23221 aagatagtgt agcacaagtt aagattaaat cagcagaaga agtgtttaca gaagtattaa
    23281 atgagataga aaagaaactt ataactatag atatgataaa tttctttata tcagaaaaag
    23341 catttgttgt agaagatgct gatataaaat cattaaagga aatactagat aaatttgaat
    23401 tagattatga agtaaataga gactgtgcta aggtaacatt gatatgttca agaattgctg
    23461 atgaaatgtc tggtatcatg tcaaaggtag ttagaggatt atcaaaagca ggggtatctc
    23521 ttttacaaac atcagactca aatatgacta tatcttgttt agttagtgaa gaagatatgc
    23581 atacagcagt acatgctata caccaacaat tttatttaaa ataaactgta taatacaatt
    23641 attttttaat caaatttaag aatttcatat atatcttaat atatttgatt tatttggtaa
    23701 aactaaatta aatataattt tgataggagt ttttttatga aagattatag agaagataat
    23761 gaaagatttg ataaagataa ttgtagtgaa gaatctagtt gcgaagagag agaatgtata
    23821 aagcaatttg gtaccaatga aatgccacct caacctccta aagatattca atgtataact
    23881 ataattggag aaattgaggg acattttata ggaaatcctc aaaaaaaagc tacaaagtac
    23941 gagcatataa ttccaatgct atactcaatt gaggaaagca atgatgttaa aggagtgtta
    24001 gttgttttaa acactgttgg aggagatatt gaagcaggtt tagctattgc agaactttta
    24061 aacagtacat ctaaaaaagt agttactctt gttctaggag gaagtcatag tataggtgtt
    24121 cctcttgcta cagcaggaga ttactcattt atagcaccaa cagcaaccat gattatacat
    24181 ccagtgcgaa ctactggtct tgtgattggt ataaatgaga cttttgaata ttttaaaaag
    24241 atgcaagata gaataattca atttataata agaacatcaa atatcaaaaa agatgtactt
    24301 gaaaaactta tgcatgaaaa agatgaactt gtaagtgatg ttggtagtgt attaatagga
    24361 aaagaagctg tggattatgg tcttatagat gaagttggag gactaaaaga agcacttaaa
    24421 aaattaagag agcttataaa agaatctgag gaagaaaaaa ataatgatta aaatttgttt
    24481 aaataataaa atttatgata taatgatgta ataaagtagg agtgttagtg ttaaggcatt
    24541 agcacttttt gctgtgaagg ggtaaaactt gctaaaaata aggagtgata ttaatggcca
    24601 cgaaaaagaa gaaaaaaaag aaggtaaata ccttaagttt taatgcagaa tatcataatt
    24661 taataacaat atttataggt gtatttttat tatatagttt aaattctaat tctatgggat
    24721 ggatacctgt gttaatgcaa aatcttttta agggactatt tggagggctt tcaatagcaa
    24781 taccatttat agtaattata acaggacttt taggattttt tgatggaaat gaatatatat
    24841 ataggctaag aaagactaaa ctatactaca taattatttt atttatattt gtattttatg
    24901 gattattaaa tgcaggaact cttcctgtag atagcccact aaaaggtaac atgtttgatg
    24961 atgttatgaa attgggtgta agtggacaag gtagtggttt gatagcaaca actattgcgt
    25021 attatatgag taaaatattt ggtatagcag gtggatggtt aataagtatc ttcgcactta
    25081 tactttctgt aatgtttata tttaatattt ctataaaaga tttggtgtca aatgcaaaat
    25141 caaaatctaa agcatctaaa gacagcaatt taacgtttaa agataaaatt gcaaatatga
    25201 aaaaatctgc aatagatatg atgactgatg aagtagatga tactactata aataaaaaac
    25261 caggtttctt taaagggtta atgtcaaaag gtcttaataa tgatgatgaa gaagatgaat
    25321 attttgaaga agaaaatagt gatggtgtag atgataaaac tataaaaata gttggattta
    25381 ataaggcaga agatgagtat ttagaaatat tagaaggaac tcaaagtatg ccagagttag
    25441 atgttttaaa agaattacaa aaagctacca atgaaaatcc agttgtagat actaaaccag
    25501 aaaaaaaagt tgatatagct aaatctaatt taaatataga aaaaactcaa cctatgagta
    25561 ttgtagcaga gccagttaat gaggattatt caaactataa aaagcctagc attgagcttt
    25621 taaataaagt aaataagaag tctgatgaaa atggtaaaaa gaaagtgttg aaaaatgctt
    25681 ctcttttaga aaagacacta tcagattttg gagtggaagc aaaaattaat caagttacag
    25741 ttggacctac tataacaaga tatgaaatac aaccaagtcc aggagtcaag gtaagtaaaa
    25801 ttgtaaattt aactgatgat atagctttaa gtttagcagc taaaagtata agaattgaag
    25861 ccccaattcc tggtaaaagt gcaattggaa tagaagttcc aaatgaagaa gctcaaatgg
    25921 ttggtgtgag agaagttcta gaaagtgaag aatttaataa ttttaactct cctcttgcaa
    25981 tgggacttgg aaaagacgta gcaggaaaaa ttataattgg agatataggt aagatgccac
    26041 acttactaat agctggttct acaggttcag gaaagagtgt gtgtgtaaat acattaataa
    26101 gtagtatttt atataaggca aatcctgatg aagtaaaatt actattaata gacccaaaag
    26161 tagttgaact tgcaaattat aatggaatac cacatctatt gataccagta gttactgacc
    26221 ctaaaaaagc tgcaaatgct ttaaattggg cagttacaga gatgaataga aggtataaat
    26281 tatttgcaga tgctcaagta aaagatgtaa caagttataa tgagaaagcg gaagaaaaac
    26341 ttcctaaaat agtaataata atagatgagt tagcagattt aatgatggca agtgcaaatg
    26401 atgtagaaga ttatatttgt agattagctc aaatggcaag agctgctgga atgcacttga
    26461 ttgtagcaac tcaaagacca tctgttgacg ttataacagg ggtaataaag gctaatatac
    26521 catctagaat agcatttgca gtatcatctc aaactgattc aagaacaatc cttgatatgg
    26581 gtggtgcaga gaagcttcta ggtaaaggtg atatgttatt ctatccactt ggtgcagcaa
    26641 aaccagttag acttcaaggg gcattcatat cagagagtga gtcggaaaaa gtaattgatt
    26701 ttgtaaaaag tcaggttaaa gatggaataa aatatgaaga agatataatt gaaactatat
    26761 ctaaggttaa tacttcaaag agtagtgatg aggatgagtt tttgagtgaa gcgatagaat
    26821 ttgtcgtgga aagtggtcaa gcatctgcct ctatgctaca gagaagattt aaaataggat
    26881 tcaatagagc agctagactt atagattcta tggaagaaag aggcataatt ggagcaagtg
    26941 agggaagtaa acctagaaag gtattaataa gcaaacagga tttgcagaat ttagaaggtg
    27001 aatagaattg tcagttataa aaatagaaaa agcacttaag ctagataaag taatatttgt
    27061 agatgtgaga actgaaggag aatatgaaga agaccatata ttgaatgcaa tcaatatgcc
    27121 tctttttaaa aataatgaac ataatgaagt tggaacaatt tataagatgc agggaaaaca
    27181 tgaagcaata caaaaagggt ttgactatgt atcgtataaa ttaaaggata tatatttaca
    27241 agcagcagaa cttgcattaa attatgataa tattgtcata tattgtgcaa gaggcggaat
    27301 gagaagtgga agtatagtaa atttattatc atcattaggt gtaaatgtat atcaacttga
    27361 aggtggatat aaggcttata gaaattttgt attagagtat ctaaggcatg taatggatac
    27421 aaaaaacttt gtatctgttc atggattgac tggtacagga aagactgatt tgttacattt
    27481 attagatgaa aaaggaatag atgtactgga tttagaaggc atagctaaaa acagtggttc
    27541 tacctttggt tttataactt ttgataaaaa gccaccatct caaaagaact ttgaaacaaa
    27601 actatttgaa agtgtatttt tttcgaaaga gaattatata tttgtcgaga gtgaaagcaa
    27661 acgagttgga agtgttacag ttccaaatga aattttcgag gcaatgacac gagaaggaca
    27721 tcatatatta ttagaatgtg atattgaaaa tagagttgat agactttgta gagattatat
    27781 ttatgataaa gatgaaggta atatattgat tttaaaagaa tgtataaaca agtttagaaa
    27841 aagattgggt aataaagtag tagatgatta tattgattta ttagaatcag gtgaatataa
    27901 agaacttgta aaaagatatc ttttagaata ctacgaccca ctttatatgc attcagtaga
    27961 gaaatataag tatagtaaga ttataaattt taatgataca caaaaagctt tagaagatgt
    28021 tatagactta tatgagagta tattggaagg agaaagtgaa tgttaaaaat agcattggaa
    28081 tcattaggat gctcaaaaaa tttagtagat gcagaaataa tgatgggaat tttaaataac
    28141 aaaggctata aattaattgg agattttgaa gaagctgatg taattatagt aaatacatgt
    28201 gggtttatag agtcagctaa acaagaatct atagatacga taataaactt ttcagagtta
    28261 aaaaaaactg gcaatttaaa gttgttgata gtaacagggt gtctagccca aagatattct
    28321 gaagaattaa aaactgaaat tcctgaaata gatgctatag taggaacagg aagctatcaa
    28381 aatatagata aaatacttaa agaattaagt gaaatgcatc aaattgtaag ccttaatgat
    28441 atagagtttg tatttaatga agatttacca agatatatat ctacacctag ctatatggcc
    28501 tatttaaaaa ttggagaagg atgctccaat aattgtactt attgtataat accaaaacta
    28561 agaggtaaat atagaagtag aaaatttgaa gatataatta aagaagctaa gaaattagct
    28621 gaatctggag taaaagaact tgtggttata gcacaagata cgacaaagta tggttttgat
    28681 ttatatggaa aagaaagatt atcagaactt ttagaagaac ttgcaaaaat agatggattt
    28741 aagtggataa gggttatgta ttcatatcca gaatctataa cagaggaatt gatacaagtt
    28801 ataaagaaat atgataatat atgtagttac tttgatatgc caatacagca tgcaagtaac
    28861 aatattttaa aattaatgaa tagaaaaact actaaagaag atatattaaa taaaatcaat
    28921 ctgataagaa gtaatatacc agatgctata cttagaacta cgataatagt agggtttcct
    28981 ggtgaaacag aagatgactt caaacaactt gtggactttg ttgaagaggt taaatttgat
    29041 agacttggtg catttgcata ttcaagagaa gaagatacac cagcagatag attgccaaac
    29101 catatagatg aagaagtaaa aatacaaaga agagatactt taatgatgat tcaacaaaaa
    29161 atatctgaag aacttaatga caaaaagata ggaaaaactt atgaggtgtt aatagaagaa
    29221 cagatagaag ataatgtata tacaggaaga acacaaggtg atgcggaaga aattgacagt
    29281 atagtgtatg taaaaagtgt agacaatctt gaagtaggag aatttgtaag tgttcaaata
    29341 aatgatgcaa tggaatacga tttaatggga gatgttttat atgaacttgc ctaacaagtt
    29401 aactttgttt agaatatttt taataccagt atttgtttta attatgttgc tcaatgtacc
    29461 aaataaattt ttaatagctt gtattatttt tataatagct tcaattactg atgctttaga
    29521 tggaaaaata gctagaaaat acaatctagt cactgacttt ggaaaattta tggacccatt
    29581 agcagataag ttattggtta tttcagcact tacatgtatg atagaagacc atcttgtaag
    29641 tagttggatg gtaattataa tagtggctag agagcttaca gttagcatat taagagctat
    29701 agctgctgct gatggaaaag taattgctgc tggaaacagt ggaaaactta aaacgataac
    29761 tcaaatggta tctatagtgt ttttattgtt aggcgctcag tttgaaaatg tattgatatt
    29821 aaatattggt gaaattctca ttcttatagc tacactactt acattatatt ctggatggga
    29881 atatctatat aaaaataaag agctttttat gtcttctaag taaaaaatta tgctttaata
    29941 aataaaaact aatgattaat aaataaaaaa taacaactta aaattttaaa aatgtaaaaa
    30001 agggtatact attatagtat acctcttttc attttaagaa tattttcttt tgtgtatata
    30061 attattaata atggtttata aataaaatgt aatgaataaa aatataatca ttagtagcgt
    30121 ttttaaattg actaggtttg atttatgtta tataattaat atataacaag agaacaaatg
    30181 tttgtagata aatgaaggga tggtatagag atgagtgtag atcaagaaaa attaaaagcg
    30241 ttgaatgaag ctttaggtaa aattgaaaaa gattttggta aaggttcagt aatgaaattg
    30301 ggagaagcaa catctatgtc tatagatgtt atatcaacag gagcgattgg tttagacata
    30361 gctattggta taggaggtct acctagaggg agaatagttg aagtatatgg tccagaatct
    30421 tctggtaaga ctactgttgc gcttagttgt gtagcatcag ctcaaaaaga tggaggaata
    30481 gctgcattta tagatgcaga acatgcactt gacccagtat atgcaaaagc tttgggtgtg
    30541 gatgttgata acctaataat atctcaacca gatacaggtg aacaggcttt agagatagca
    30601 gaggcattga taagaagtgg agcgatagat ataatagtaa tagactcagt agcagcatta
    30661 gttccaaagg ctgaaataga tggagatatg ggtgattctc atgtaggttt acaagctaga
    30721 cttatgtcac aagcacttag aaagttaact ggttcaatta aaaaatcaaa ttgtgttgct
    30781 atatttataa accagttaag agagaaagta ggaataatgt ttggaaaccc agaaactact
    30841 actggaggac gtgcactaaa attctattca tcagttagat tggatgttag aaaaatagat
    30901 acaataaaac aaggtgataa agttataggt agtagaacta gagttaaagt tgttaaaaac
    30961 aaagtagcac caccatttaa gcaagctgaa tttgatataa tgtatggaga agggatttca
    31021 aaaattggag accttttaga tatagctgct gatgtagata tagttaaaaa atcaggttca
    31081 tggtatagtt acaatgatac taaacttgga caaggaagag aaaatgttaa aaaattcttg
    31141 gaagataatt tagatttaac tactgaaata gatgagaaag ttagagcatt ttacaattta
    31201 aatgaagaac atgaagaatc aggtacttca gtatcaaaag aaattgtaga agaataagta
    31261 aaaatacaaa aaaataacta gtaattaaag acatgccacg ctccctatta gcttgacaca
    31321 atgttgaaaa aattataaaa ttaagtgagg ataaaatatc taaatttaat tttaattatt
    31381 aggaatattt tttaatcata gtgatgacat tttgggatta ttaacgaagg aggtggatgt
    31441 catagatagt gtagtaatga ttgtaatagg tgcagctgtt ggtattatag ctggatattt
    31501 tgttagaaaa aatatatctg aagcaaaaat tggacaagct gaaaacttgg ccaaagaaat
    31561 aatagataaa gcacaccacg actcagaaac agtacaaaaa gaaaaattac tagaagctaa
    31621 agaagagatc cataagtgga gaacagaagc tgaaagagaa aacagagaaa gaagaacaga
    31681 agtacaaaag tatgaaaaaa gagtagtaca aaaagaagaa gtcttagata gaaaattaca
    31741 aaatttagaa tctaaagaag taaacctaag tgaaaaatta aaggtagtgg aaaagaaaga
    31801 agaagaagtc gaagtaataa aaactcaaca gttagaaaaa ttagaaagta tatcaggaat
    31861 tacttctgat aaggccaaag aaatcatctt aaccaatgcg gagagagatg taagacgtga
    31921 aatgtctata atgataaaag agatagaaag tcaagctaaa gaagaagctg ataagaaatc
    31981 aagagaaatt ataggatatg caattcaaaa atgtgcagca gatcacgtag ccgaaactac
    32041 tgtaacagta gtaagcttgc ctaatgatga aatgaaaggt agaataatag gtagggaagg
    32101 tagaaatata aggactttag aaaccttaac aggtatagac cttataatag atgacactcc
    32161 agaagccgta attttatcag gatttgatcc aataagaaga gaaattgcaa gaattgctct
    32221 agaaaaatta atagcagatg gaagaataca tccagctaga attgaagaaa tggttgaaaa
    32281 ggcgagaaaa gaagttgaca atattataaa agaatatggt gaacaagctg catttgaaac
    32341 tagtgtacat gggattcatc cggaattaat aagattatta ggtagactta actatagaac
    32401 tagttatgga caaaatgtac ttaaacattc tatagaagtt gctcatatag ctggtataat
    32461 ggctgctgaa ataggagcag atattagatt ggctaagagg gcaggtttat tacatgatat
    32521 aggtaaggct gtagaccatg aaatggaagg tacacatgtt gaaataggta tggatttact
    32581 tagacgttat aaagagtcta aagaagttat acatgccatg agtactcatc atggagatta
    32641 cgaacctcaa acagtagagg ctgtattagt tacagcagca gatgcaatat cagctgctag
    32701 acctggtgcg agaagagaaa ctttagaagc atatataaag agacttgaaa aattagaaga
    32761 aatagctaat tcatatgaag gtgtagaaaa atcatttgct atacaagctg gtagagaaat
    32821 aagaataatg gtaaaaccag aagctataaa tgatgaagaa atacatctat tggctagaga
    32881 tatgactaag aaaatagagg atgaacttga atatcctgga caaataaaag taagcataat
    32941 aagagaaaca agagctatag agtatgcgaa gtaaaagaaa taatatctaa ataataagaa
    33001 aatattttta gaaatttaaa tttgccacaa gatatgataa tatatcttgt ggcttttaaa
    33061 ttgtttaagt ttaattttat ataatgcaaa aataatctaa gtgctaaaag tgctacttac
    33121 aaaagattaa taaaataaag ctttatatat gtattattta tataattatc caagtttgtt
    33181 tgatataatg aagttgtaaa atcaatactt tactacaaaa taaatagtaa atactacagt
    33241 aaaatctaaa aataggatat aatagtatta atataagaag attataaata aaacctgcta
    33301 atgaaaatag attattttta gcaggttttt aaatttttta taataaatct tatttttagt
    33361 caacaagatt tatttttttt aaaactttac ttactatttt aaacattaat ta
//
LOCUS       DAGQWR010000025        33071 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.025, whole genome shotgun sequence.
ACCESSION   DAGQWR010000025 DAGQWR010000000
VERSION     DAGQWR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.025"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            65..811
                     /locus_tag="MIW95_001054"
     CDS             65..811
                     /locus_tag="MIW95_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBY3150399.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            873..1547
                     /locus_tag="MIW95_001055"
     CDS             873..1547
                     /locus_tag="MIW95_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY3150400.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCLNCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCALRCMMSCPWSL
                     RYANQK"
     gene            1915..3261
                     /locus_tag="MIW95_001056"
     CDS             1915..3261
                     /locus_tag="MIW95_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBY3150401.1"
                     /translation="MEIETKTPDWINVLELNSKIDITWKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIVGTTNFITRGVSNI
                     SIITLEGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLFHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     REIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITKCGQYYKSVDYNTYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNKVMLTC
                     YAEADELIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSIEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3288..4688
                     /locus_tag="MIW95_001057"
     CDS             3288..4688
                     /locus_tag="MIW95_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBY3150402.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSSKLQEIIDAAPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GEPGHYENLFFSGFSGIALDMPNNSTANDITVFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKVIKMHKPEVGDIMSTIIKDENILSAHYGGTWGKIGE
                     KIEFGITVHYYKLIEK"
     gene            4898..6334
                     /locus_tag="MIW95_001058"
     CDS             4898..6334
                     /locus_tag="MIW95_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBY3150403.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPDLQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSPTETTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKDYEGYGSYKINNITIDQCGYCGFLFDSVKGSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGKETVYYYKHHEPDKTNQIK"
     gene            6678..7622
                     /locus_tag="MIW95_001059"
     CDS             6678..7622
                     /locus_tag="MIW95_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBY3150404.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7791..8723
                     /locus_tag="MIW95_001060"
     CDS             7791..8723
                     /locus_tag="MIW95_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBY3150405.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8828..9295
                     /locus_tag="MIW95_001061"
     CDS             8828..9295
                     /locus_tag="MIW95_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBY3150406.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGETMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9539..10393)
                     /locus_tag="MIW95_001062"
     CDS             complement(9539..10393)
                     /locus_tag="MIW95_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBY3150407.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10559..12523
                     /locus_tag="MIW95_001063"
     CDS             10559..12523
                     /locus_tag="MIW95_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY3150408.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLSEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSCCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFVKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLLEESI"
     gene            12523..12984
                     /locus_tag="MIW95_001064"
     CDS             12523..12984
                     /locus_tag="MIW95_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY3150409.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSDAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            12987..13307
                     /locus_tag="MIW95_001065"
     CDS             12987..13307
                     /locus_tag="MIW95_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBY3150410.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13323..14423
                     /locus_tag="MIW95_001066"
     CDS             13323..14423
                     /locus_tag="MIW95_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBY3150411.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14543..17143
                     /locus_tag="MIW95_001067"
     CDS             14543..17143
                     /locus_tag="MIW95_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBY3150412.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKHVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDYILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNVFIEESGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLILKDSGNAGDTYDFSPLKNDEDIKLSFDRVYTEKTLGYEKLVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPEKY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
     gene            17612..17947
                     /locus_tag="MIW95_001068"
     CDS             17612..17947
                     /locus_tag="MIW95_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3150413.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            18084..19361
                     /locus_tag="MIW95_001069"
     CDS             18084..19361
                     /locus_tag="MIW95_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBY3150414.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNNSQKDSNNDNNDNG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            19482..20870
                     /locus_tag="MIW95_001070"
     CDS             19482..20870
                     /locus_tag="MIW95_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBY3150415.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSESAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            21241..21954
                     /locus_tag="MIW95_001071"
     CDS             21241..21954
                     /locus_tag="MIW95_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBY3150416.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLFLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            22072..22941
                     /locus_tag="MIW95_001072"
     CDS             22072..22941
                     /locus_tag="MIW95_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBY3150417.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSITSNIITV
                     IPEKSRVEVLQVDGDWSKIVYDDKIGYVFNYFLSTDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(23096..24241)
                     /locus_tag="MIW95_001073"
     CDS             complement(23096..24241)
                     /locus_tag="MIW95_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBY3150418.1"
                     /translation="MSTYYVPPINLLGKGCLNEAKDSIKSLGTKKAFVVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVDKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIVAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAVEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKEKIIELFMKIS"
     gene            complement(24574..25350)
                     /locus_tag="MIW95_001074"
     CDS             complement(24574..25350)
                     /locus_tag="MIW95_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBY3150419.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            25932..26906
                     /locus_tag="MIW95_001075"
     CDS             25932..26906
                     /locus_tag="MIW95_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBY3150420.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            27004..27309
                     /locus_tag="MIW95_001076"
     CDS             27004..27309
                     /locus_tag="MIW95_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBY3150421.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGNEISFKLKDKTVKTITALDNI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            27465..28625
                     /locus_tag="MIW95_001077"
     CDS             27465..28625
                     /locus_tag="MIW95_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBY3150422.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            28675..29577
                     /locus_tag="MIW95_001078"
     CDS             28675..29577
                     /locus_tag="MIW95_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBY3150423.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESIFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            29657..30550
                     /locus_tag="MIW95_001079"
     CDS             29657..30550
                     /locus_tag="MIW95_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBY3150424.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNKLNIC"
     regulatory      30603..30784
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            31012..32142
                     /locus_tag="MIW95_001080"
     CDS             31012..32142
                     /locus_tag="MIW95_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY3150425.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            32214..33059
                     /locus_tag="MIW95_001081"
     CDS             32214..33059
                     /locus_tag="MIW95_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBY3150426.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KLIKK"
BASE COUNT        12966 a         3519 c         5757 g        10829 t
ORIGIN      
        1 atattaataa actttaatat aatatattta tgaaaaatgt aaattgtgaa atggaggtag
       61 taacatgaaa attgaaaatc tttctattga tgatattaaa cgaggctata tatttaatga
      121 tgatttaaat tgttatagtt gcattaaatg tacaaaaaca tatgaggtag gagaaatata
      181 taaaataggt aatagattct ttgaagcttc aaaagctatt gaacttcata tggaattaga
      241 acatagtgat tatttagagc aattgcttaa tagtgattca aagtacaaca cattgactga
      301 aaatcaaaaa caattatttc aattatttat tagtgattta ccagataagg agatagcaaa
      361 acaggttgga gtatccacat ctactatacg ccatcaaaaa tttattttca gagaaaaagc
      421 aaaacaggct aagctctatt tagcactata tgaatctgta tttgaagata aatctaataa
      481 aagtagtact ataataccaa tccatgaaca tgctcgaatg gtagatgaaa gatatgtagt
      541 aactgaagaa gagagaaaac acattttgga tacatcattt gaaagtatga atcctttaaa
      601 attaaaagta ttttcttcaa aagaaaagaa aaaggtcgta attttatcta agattttgga
      661 acaatttgaa cgtgggaaga aatattcaga aaaggaagtt aatcagattt tagaatctat
      721 atatgatgat tttgcaacaa ttagaagata tttaattgag tatggcttta tgagtagaaa
      781 taaagaatgt acaaagtatt ggttaacata attaaaataa taaattttac taatgtgtaa
      841 tgttattttt atctaggggg gaggagttaa atatgaatga tgaattaaaa tctattttat
      901 acaataaagg tgtagacatt gttcgctttg ttgatatatc tgaatttcca ataaatcaaa
      961 cacaaggttt ttctaaagca attttatttt gtatagggtt atcaaaaaaa tttattacag
     1021 atatatacaa taacttacct acagatagtg atgagttttt ggaaaaagag gagaaagtag
     1081 aagaattagc tgactggatt tcaaaatata ttcaaaataa aggatataga gcgtattctc
     1141 aatcggagaa aaacaatcta gaacatggat attttgaaaa agcctatatt aatccagaaa
     1201 tgcaatcagg gattagtcct ttaccacata agacaatagc aaatattagt ggcattggat
     1261 ttatgggaaa aaataatttg tttgtaacag aggaatacgg atgtgctttt agcatgtgta
     1321 cagtacttac agatgctcca atttctgttg aaagatatcc tttgattgat tcaaaatgtt
     1381 taaattgtaa tgtttgtgta gagaactgtc ctgcaaaagc tatacatgga aatgaatgga
     1441 ctttacctgg aaaaagagaa tctattattg atgtttcaaa gtgtttttgt gcattgaggt
     1501 gtatgatgag ttgcccatgg tcattaagat atgcaaatca aaaataactt tactagagaa
     1561 ggtattaaat tgaatattat aaaattttta aaatgtaaga ttatataaga tttctcaaaa
     1621 taaaaatttg tatagaacac tcagaaatga gtgtttttct attgggatta aattatattg
     1681 atacaaagct taactatttt aatcattata taaaatttgt attaaaaaat taagaataac
     1741 ttgctaatag taaaaatata atgagattat aattgattta taatataact gtaatgatgt
     1801 tgtaaaatga aaattgtcat ctcaaaattc ccaaaaagag taaaagtcac ttcggttata
     1861 gtcaaaaaat tttttctgtg aaaagaattt agatttaaaa gggaggaatt aatcatggaa
     1921 attgaaacta aaactccaga ttggattaat gtactagaat taaacagtaa aatagatatt
     1981 acttggaaat atgatgtaag taatatgata caagatattg tctctgataa gtcactagaa
     2041 ggttccatta tatattttcc aaaaggtaat tacttatttg agaaaggtat taaaattagt
     2101 caacaaataa cacttcaagg agattcttat tatggaggag ataatcaagt atcaaattta
     2161 gataaaaaac aatcaatcgt aggaacaacc aattttataa cccgaggagt ttctaatata
     2221 agtattatta cattggaagg aacaagtcaa tgtatcaaaa atattaattt ttattatgat
     2281 agtcatgata tagagaaaat accacctaaa aatgtatctg ctattacaga atatggagaa
     2341 acacaaggac tatttcactt tgaacacctg tttatttctg gattttcagg aatagggatt
     2401 gaaattccat actattcaac tggcaatgat atcattgtta gttcatgtgg tcttggtatg
     2461 aggcttggag agaagtcaat gctttctagt agcaagattt atgaatgtaa aaatggaatg
     2521 gagattacta ctggtgttag cttaaataat gtgagaatag aagaaataag agaaataggt
     2581 attaataata aaggatttgg ttttaacctg attatgaatc ttatggtcga ccaatgtgga
     2641 tattgtggat ttatgtttga aaaaatgtgt tatagccaga taactgccag aattactaaa
     2701 tgcggtcagt attataaaag tgttgattat aatacttatg agaatatgga agatagaaaa
     2761 gaagaagctt attccatttt ttatggagat attttagaaa attgcaatat tgtattgatg
     2821 aatagtaata ttgatacctt agaaaatgat tcacttgatg aacataagat atatgtaatc
     2881 aaggcaaatg aaacaaacaa agtaatgtta acatgttatg ctgaagcaga tgagcttata
     2941 cagtgcgcaa agggcaactt gttgctagaa aatggtgaaa atacttataa attttataat
     3001 ggtgagattt gttctgtagg aggtgttcaa atttctgata tagatgaaaa agatttaatc
     3061 aaaataatcg agaacactct gtatatagat gataaaggta agtacttaat ggttcctaga
     3121 ttaaaaaaga atactgtaat atattcagta ctagattcta tagaagaaat aaataaatta
     3181 catggtggaa tatgggaaca gattgatgta aaagtattag ctggggaatc tatgtactat
     3241 tataaaaaga taaaagatta aattatcaaa ctaggaggta gtgattaatg gaagagtcta
     3301 taataattaa agatataagt ggctggatta atgtacttga ttatgggatt tgtaatgatg
     3361 aaacaaaaga tgtaagttcc aagttgcaag agattataga tgctgcacca gaagattcta
     3421 ttatatattt tccaaaagga aaatacttat ttgaaagtgg tgttaagatt aagaaacgat
     3481 taaccttatg tggagattct tatattttgg cgaatgttcc taaaaacact gctggagtaa
     3541 cacaatttac ttttaaagga aagaaaaatc ctaaaagtga aaatatagca attataacag
     3601 caaaaagtgt aagacactgt attaagagca tttgctttta ttcagatagt tgtgatagag
     3661 atatgacatc taatcatgaa cctccaacag atggaaaacc tagatttcat cataatgtaa
     3721 ttataaatta tagaaatata tcggctattg tatgtgaaga taaacaggga gaaccaggtc
     3781 attatgaaaa cttgtttttt agtggttttt ctggaattgc acttgatatg cctaataatt
     3841 ctacagcaaa tgatattaca gtttttacat ccggacttgg aattagtaca ggggaaaatt
     3901 caacgattgc taacagtaag gtttggggct gtgttgatgg aatgataatt tctactggaa
     3961 cttttataaa tggtatgagg gtagaagaaa taaaaaaagt tggaattaaa aatattggta
     4021 gaggtttaaa ttttattaca aatatcacaa ttgaccaatg tggatattgt ggagtttggt
     4081 ttgattctat atccaatgtt cagatatcag gaaatatcac aaggtgcgga cagtactatt
     4141 atgatgtaag ctatgataat tatttaatgt tggataatag agttaaagaa gcatattctt
     4201 tattctatgg taatttattg gagtcaagta atatagtttt gtcaaatacc aataaagata
     4261 attgggaaga tttcaaagag gataagcata aggtgtatct tgtagaagca cttgaaacca
     4321 agaatgtact attaacatgc aatatagatg ctactgactg gattgttaag tcagaaaaag
     4381 gaaatttaac gtacaataat atgcgacaaa ctgttatatt ttacgatgga aaactaagta
     4441 gtatcaatgg aattggtata tctgatgaag attatgggga taaagtcaag ataaaaaaag
     4501 gaaatatata tataaatagt aaggataagg ttattaagat gcataaacca gaggtaggtg
     4561 acattatgtc aacaattatt aaagatgaaa atatactttc cgctcattat gggggaacat
     4621 gggggaaaat tggtgaaaaa atagagtttg gaataactgt acactattat aaattgatag
     4681 agaaataagt aaatacgtag tatttgaata gatgtatttt attggagagc taggactata
     4741 gttttaatgt aaatggtttg gtaatagcat ggattgaata atataaaaat aatataaaaa
     4801 attaatttga ttatgtaaaa ttctatgaag tagtaaacct gtgactttag tcatggtagg
     4861 ttcaaattaa gtaaatggga aaggaatata taatattatg gaaggaagaa ctgggattaa
     4921 agatggaaga tggattaatg tagtagaaag aggtattaaa aatgatggtt cgtacaacat
     4981 aggtcctgat ttacaggata ttattgatga aatagaaaca gatttctgtg tcttatattt
     5041 tccaaaagga aaatacttat ttacaagtgg tgttaaaatt aataaagaaa ttgtattaca
     5101 aggtgattct aattcaccta cagagactac acaatttata actcgtggag tagaaaatat
     5161 gagtattatt acattgacag ggaagaaaca atgtattaaa aatataaatt tctattctga
     5221 tagttgtgag atacaggtaa atgaagaacc accgacaaaa ggtaatccta aatatcatta
     5281 tgagatgatt attaataaga atgaaaaaaa tgttgagatg aataatgtat cagcaattct
     5341 gtatgataat agtaagaata tgattaaagg tctaggtcat tatgaaaata tttatataag
     5401 tggtttttcg ggtacaggaa ttaggatacc atattactca attgtaaatg atattacagt
     5461 ttcatcttgt gggttgggta ttgatacagg aatagacaca attatttcta atagtagaat
     5521 atctaagtgt caaaatggaa tgaggattag aactggaact agcataaata atgtaagaat
     5581 tgaaaaaata cagaaagttg gtcttataag tagtcaagtt aaggattatg agggatatgg
     5641 aagttataaa attaataata ttacgattga ccaatgtggg tattgtggtt ttttatttga
     5701 ctcagtgaaa ggtagttatt tttctggtat tataagaaga tgtgggcagt attattataa
     5761 tacagattat gacacatatt taagtattaa agatagagta gaagaagcat attctttatt
     5821 ttatggaaat tattttacag aatgtaatat tgatttagtt agtgataata aggataactg
     5881 ggatgatggc ttagaatata aacataaagt ttatgtattt aaaacccttc aaataccaaa
     5941 attaacattg agatgcaata tagatgccta tgattatatc gttaaatcgg tacatggagg
     6001 aggtaattta atgttaaaaa ataactttaa caattatata tttattgatt catctagtcc
     6061 agaagttatt aatggaatta attttaatta tcaaaagaat ggaaacctgt taaggttaaa
     6121 taatggtaaa ttaagttata aaaatggaaa ggaagaaaca gctatattca gacctaatta
     6181 tggtcttatt gtagcatcaa cagattcaga atgtgaacaa atagagacta gctatggagg
     6241 aaaatgggag aagcttggtt ctgaagtaat tggaaaagaa acggtatatt attataaaca
     6301 tcatgaacca gataaaacaa accaaataaa ataaaatttg tattatataa tgatataaag
     6361 ttttaatata tagtgtttac ctttaatgta atattggtat atttgtatag tattaaattt
     6421 gttaaaaagt aaaattgtag ggtgcttgaa aatagcacct tttttagttt cattataaaa
     6481 ttagttttga aagtttagga ataatgtata tttattttta aagaattata accttattaa
     6541 ggatatttta aatgctagaa atgaatcagt aaatcatact ggaattatct taaatgtttt
     6601 gattaaatta agtatttaga gctataatta tattaaaaat atattaggac ataattatat
     6661 tactatggag gttaaatatg atagtaagtg tgagtagaag aactgatata cctgctttct
     6721 atagtaagtg gttttttaac agacttaaag aaggttttgt ttatgtaatt aatccaatga
     6781 atccaaaaca ggttagtaag atagagttaa atccacatac tgttgattgt tttgtatttt
     6841 ggacaaagga tgtaactcca atgttatatg atttaaataa attaaaagac tataaatatt
     6901 attttcacta tactataaca tcatatggaa aagaagtaga gacatgtatt ttagataaaa
     6961 gaaaggtaat agattcattt aaggaattgt ctagaaaaat aggcaaagaa agagtaattt
     7021 taagatacga cccgattttc ttaagtgaaa agtacacagt agattatcat atcaaagcat
     7081 ttgcgagttt atgtaatcag cttgatggat ttacagaaaa atgtataata agttttatcg
     7141 atttatataa gaaaacaaaa tttaatacta aagcattaca tataaagcca atagaaatta
     7201 cagaaataga aacattatca aaagaaattt ctaagattgc tcataagcat gggataatac
     7261 ttgaaatgtg ttctgaagaa tataatttaa gtaaatttgg gattggaaaa agtaagtgta
     7321 ttgatgatag attaatatca aaaattatag gatgtgaagt agaggtaaaa aaagatagta
     7381 cacaaagaga tatttgtgga tgtgttaaaa gtgtcgatat aggacaatac aatacttgca
     7441 gacattattg tctgtattgt tatgctaact ttaattatgc aaaagtagaa gaaaactgta
     7501 ggctttataa agaagataat aaattactca tagatacttt aagagatgat gcaaaaataa
     7561 gcattagaga tatgaagact attaaagtag ataagaataa acaaattagt atatttgatt
     7621 aaaataaata tactaattac cttaatatat caagaatata gatacttttg ataacaaaat
     7681 taaacttaat agatattttc tatataagaa agatggtcat aatatgtcaa tttgaccatc
     7741 tttttgatat aattaaaaca caaataattt agtaacaaag gaggcagaac ttgaatttat
     7801 taaaaaaata tcctatacct attgcaagtt tgattttgtc aatttttact ttaggtaact
     7861 tattacaatc ttatagtgaa tcattacgta atgtaattgg atttatagga tttattttgt
     7921 atgcaattta tatagtcaaa ataatattac ttagaaaggg tgttaaagag caattagaga
     7981 accctttaat atcaagtaca tttccaacta taacaatggc aacaatgata tttgctacat
     8041 atataaaacc attatcttta gaattaagta ttatgatttg gagcatagga attgtgggtc
     8101 atcttgtgtt aatagtttta tttagtaaga agttcttagt taaattctca ataaaaacgg
     8161 tatttccatc ttggtatata gtatatgttg ggatagttgc agctagtgtt acggcaccag
     8221 catttaatca acttttgatt ggcaaaattg ctttttggat aggatttgta tcttatatag
     8281 ttcttatacc agtaatttta aagagagtat ggataatcaa ggaaatgcct gaaccatctt
     8341 taccaagtat agctatatta gctgcaccag ggtcactatt attgtctgga tatattaatt
     8401 ctttttcaga taaaaataat gtagtattat acttgttatt catattatca attgtatttt
     8461 atattatagt tttgggatac ttaccgaaat tattaagatt gcctttttat ccgagttttg
     8521 ctgcatttac attccctatg gcaataagtg caatgggtat gaaactaatg aatggttatt
     8581 taactaaatc aggtcatttt ataagttgga tttcatatgc ttctaagtta gaagagataa
     8641 ttgctacagt gattatttta tatgttttag ttttatttat tatatatctt agtaaatctg
     8701 aaactacatc taaagctaaa taagaattat taaatatgtg tatttaatag gaatattgat
     8761 ataaatatat gaagataaga taaaccatag aaacttaatt tagcttaatt ttattggagg
     8821 aaaacaaatg aaaacaaaag aagaatattt aaaagaagtt agaaacatat cagaagaata
     8881 ctttagaaaa ggagagtttt tttgctctga agcagttctt cagacaataa atgatgcttt
     8941 aggccaacct ttaagtccag agataactaa attagcatca ggatttccga taggacttgg
     9001 aaaagctcaa tgcctttgtg gagcgatatc tggaggagag atggctttag gtatagtata
     9061 tggaagagta catggagaaa ctatgaatcc aaagatgttt gagcatgcta aaggtttgca
     9121 tgattatatt aaaaaagagt atggtgcaac atgttgtaga gctattacaa agaaatggga
     9181 tggtgataac tttatgtctc ccgaaagaaa agagcactgt attaaaataa caggtcaagt
     9241 tacagagtgg gttgcagata agcttataga agatgaaaaa cttaatatca aatagtgtaa
     9301 tattagttgg tgaatttata aagttaaatt tacttatagt ataagatact tgatattctc
     9361 gtattgatag aaaaatttat agcatatgta tttaatgaaa tatacttata tttagagtgt
     9421 ggtttaattt cataaataca tatgctatta ttttaagtat ataagcaaat ttatttaaat
     9481 aaatagaaac agttaattta tatgtacaaa actatatatg attagtgtaa ataaatacct
     9541 attttataat actcaacata aagacaccag acatgattag tattatacca ataaaaatag
     9601 caagccattc ttttttgttc ttcttttctt taaaaaatat aatccctcct aaagtggcta
     9661 ctatcacaca actctgagag atagaaaaac ttattgtaac tcccaatgta ttagtcgtta
     9721 tgaacatacc taaatttgct attgaccaca atataccagt aattaaattg aataaaacat
     9781 tttccctata tacaatctct ttagaaaaat acttgacaat aatagatgaa gttatcaaca
     9841 taccaacaga ttgaggtaat ataattctat atccttcaat taaaaatatt tgattagtta
     9901 caacatataa tgataaaaat agagatgata ataatacaac tccaacacat tttaacatat
     9961 ttttatctgt agatttttta gtttgataat tagtgagaag tataccaagt attatacata
    10021 atattgataa agaaccaatc agtaacattt tagaactggt ccattcatta aacaagatta
    10081 cagctaataa tgtcgtaaaa gttaattgtg aaccatttga aattggcatt actttagata
    10141 cactagcaat ttgaattgct ttaaactgca ttaattgtcc caaactccag aatatgccag
    10201 aaatcatact tatacaaaat gaaaaaaatg taatttcagg atgtataacc agagttatta
    10261 tagtggaaaa tataaacgca cttatggatg tacctaaaag ctgttcatga ggagaacttt
    10321 tctttagatt tgcaactatg ggcatcaatc cccatcccat tacaggaagt aaagcaataa
    10381 ttattgtcag cattaaacta tttcctttca tattaaattt agacagcttt taatttatca
    10441 tctaaaatta aagtgttcaa tacataaaaa ttaccgtatt taatcaggaa atattagtag
    10501 ttttttagaa aaacattttc cattaaaata aagataaagg gaaggagtaa aattgaaaat
    10561 gttaactgat atgattcaaa atcgtgaaat taaaataata aagtttttat taaaagaagg
    10621 tcatacaact gtaaaagcaa tagcagatga tttagatgtt tcagaaaaaa cagtttcaaa
    10681 atctcttaaa gaagtagaag cagtgcttaa aaagataggt ctttctttaa taagaaaacc
    10741 taagataggt gtttatattg atggagattt aaaaaaaata cttccttacc tagataataa
    10801 aggaagtcaa attcctacaa ccagagagga gagagttatc tatatatatt cagtactttt
    10861 aaagagtaat gattatatta ctatacaaca actttctgat gaactatata taagtcgtgg
    10921 aactattgaa agggatttat cagaagttga aaaactgcta aaaaacgaag ggatttccat
    10981 atataagaaa cctagtaagg gaataaagtt aaatataagt gaaagagata aaagacaact
    11041 tactgctaag tttatacata aattttggag tagaaactgg tatattaagc aagagcaaga
    11101 aagcttttat caagcatttg aaaagataca ggcagatgta aatggtattt tttcggaaga
    11161 tgatttatca ataattattg atattcttag gaatttcagc agagaaagaa aatttgagtt
    11221 ctctgattat gaatttcagt cattagttat tcatttggca atagctgtag agagaattag
    11281 aaaaggcgaa tatatagaag aaagtttgta tattggtaat atagaaaata atcaaatgtc
    11341 aaataccaat tatttagcaa aacaattaga agaaaaattt tcaatattat taccaaaatc
    11401 tgaaattgga tatataaatt tacatttagt agcggctgac caacatagag atactaatga
    11461 actaaaaaat acttcaacta atccttgtga aacaagtaca aataatatat caaatcttcg
    11521 ggaaattata aaaaatacat tgtatgagac agcttatgac aaggatttaa ttgatggatt
    11581 gacttcacat gtacattcat caataaatag actgaaatat ggacttagta ttaaaaaccc
    11641 atatgtaaat acaatcaaac aaaacttttc tttatcattt gagcaagctt tagatttaaa
    11701 gaaagtaatt gaatttatat atgatattag aataaatgat gatgaatgtg cttatatagc
    11761 attacatttt gaagctttta gagaaagaat gaaagaactt ccacaaaata ttagagtaat
    11821 tctagtatgc agtacaggat taggttcatc taggttattg tctgcaagaa taaaaaaata
    11881 ctttccaatg ataaagattg aaaaagtact ttccgtacaa gaattagtat ccagtaaaat
    11941 tgatgcagat ttagtaataa gtactattta tttagagtta gaaacaattc caaccatagt
    12001 agttagccct attttaaatg aaagtgatat aagaaccttg gaaagaaata tacaaaatgt
    12061 ttcatgttgt acttctaaga agtataaatc tttcagaaac ctaatctttg aagaaaatat
    12121 attgttaaat attgaagctt caaactatga agatgcaatt aagtatataa cagataacct
    12181 aataaaaaaa ggttttgtaa aagaaggtat agctaaaagt gcaattgaga gagaaaattt
    12241 atcttacacg tcttttcagt caatagctac acctcatgct aatcctagtt atattaaaaa
    12301 atctaatata tctgttgcaa cgttaaaaaa tcctattaca tggggaaatg aaaaggtgga
    12361 agttattttc tttatttcat tagaaaatga caaaaaccta gagcttgatg aaatatatga
    12421 agttttttat gactttattg acaataaaaa aaatataaat gacatattaa gtgcaacttc
    12481 cacaaaggaa gtgtacgatt tgctattgga ggagagtata tagtgaaaat atcagatgta
    12541 ttgaaaaaag agcaaattgt ttttaatctt gatacaaata caaaggaaga aacaataaaa
    12601 aaattaagta gtatatttgt tgaaaatggg attgtggaca atgaagaaga atatgttaag
    12661 tctgttttgg aaagagaaga acattcaact acaggtattg gtaatggtat tgctattcct
    12721 catggaaaaa gtgatgctgt caaaaaatct tctataatat ttgcaaagac taagcataag
    12781 attgagtggg atgctttaga tgaaaaacca gtagatagta ttttcttatt ggctataagc
    12841 aatattgata aggatagcaa tcatcttgta ctattatcta aattagcaac taaacttatg
    12901 gatgatgaca atgtagatgc actaaaatta gccaattcag aacaagaaat aatcaatata
    12961 tttagtgaag aaggagagat gtagaaatga aaagaaaaat tatagcagtt acagcatgtg
    13021 caacaggagt tgcacataca tatatggcag ctcaagcgtt aaagaaagct tcaaaaaaga
    13081 tgggtcacct tataaaagtt gaaacacaag gagctacagg aatcgataat gagttaacta
    13141 caaaggatgc acaaattgca gaagtagtaa tatttgcagt agatactaaa gtcagaaatg
    13201 ctgaacgttt tgagggaaaa gaaatactag aagtaccagt taatgcacct ataaaggatg
    13261 cagagaaagt aataaatgat gcattaaagt taattgacac taaataaatg gagggaaatg
    13321 taatgttgaa aaagatagtc tctgagttaa aaaagcatgt cttaactgga atctcatata
    13381 tgattccatt agtaattgca ggtgctatga taatggcaat atctcgtgta ggtggttctt
    13441 tctataatat accagatata tgggatgcta aatacgcaga aagtgctagt agtattgtta
    13501 gattattaca tgatctagat ggatttggag gtactgcatt aggattaatg tttcctgtaa
    13561 ttgctgcatt tataggattt tctatagctg ataaattagc tattgtacca ggtcttgttg
    13621 gaggtatgat tgctaaagat ataggtgcag gatttttagg agctttggca gttggtctta
    13681 tagcaggata tacatgtttg tttattaaaa atcatgttaa attaccaaaa tcagcagctt
    13741 caatagtacc aatatttata gtaccagtat ttggaacatt aatcactgtt gtattaatca
    13801 attatgtgat aggtatacct tttgccacat taaatactgg attagaaaat tggttaaatg
    13861 gattatctgg tacaaatcag atattaatgg cagctattat aggggctatg atggcagttg
    13921 acttaggtgg accagttaat aaagcagctt taacaacatc acttgcatta ttaacaagtg
    13981 gaatttatgc acctaatact gctgcaatgg taggaatagt tataccacca ctaggattgg
    14041 gacttgctac tatactagca aaacaaaaat ataataaaca gttacgtgaa gctgggaaat
    14101 catctcttat aatgggttta attggagtaa gtgaaggagc aattcctttt gcggtagaat
    14161 ctccattaaa agtaatacct tcatgtgtaa ttggaacagc aattgcttca gctatggcag
    14221 ttggtttagg ttcagtaaat gcaactccaa ttagtggatt ttatggatgg tttacagtag
    14281 agaattggcc aatgtatgtg ttatcaatag cagttggaac agtaattgta gctggattag
    14341 ttatagtatt gcgtggaaat ccagtgattg aggatgatga atttgaagat gatgattttg
    14401 atgaagcaga gtgggaatct taaaatttaa taactattta agacgtgtta taagctatat
    14461 tcaatatgaa tcttatacta aataaagtgt taaatattca gtttagactt aaatataaaa
    14521 gataaagaac agaggagaat aaatgataaa agcacatatt gttaatcata cacactggga
    14581 tagagaatgg tattttactt ctggagatgc attagtactt agtgaacaac tatttacaga
    14641 tgtaattgat gaattagaac gtaatccaga tgtaagtttt gtacttgatg gacaactttc
    14701 tattttggat gattatgtac aattacacaa agaaaaaata gaaatcatta aaaagttaat
    14761 aaaagaagat agactacata ttggaccatg gtttactcaa actgatgcat tctttgctag
    14821 aggagagtct attttaagaa acttaatgat tggaattttt gaaagtaaaa agtatggtaa
    14881 gtatatgaaa attgggtatt tgccagatac atttggattt aatgcacaga tgcctatact
    14941 attaaagcat gtaggtcttg ataatatatt cttttggaga ggtatacatc ttggtaaaca
    15001 tgttcaatca ccatatttta agtggaaaag cttaaatgga gaaagttata tatatgcagt
    15061 aaatatgcca catggatatg gaactggtat gcttttagag ccttcattaa agtatgttaa
    15121 tggacgctta gacccagcta ttgattttat taaatcatat actgatgtag atgaggtgtt
    15181 aataccttct gggaatgacc agttaaatat aataggtgat ttcaaaaata aaattaaagc
    15241 gattaatgat atcggaaagt attcatatat aacgagttca tatcaagagt ttttaaaata
    15301 tataaagtcc atagaatcac tagaaagcta tagaggtgaa tttagagaac ctgtattagc
    15361 aagaatacat aaatcaattg gttctagtag aatggatata aaattagcat gtgataaact
    15421 agaaaacaag ctgattaaaa gaatagagcc attattagta attgctaaaa agagtaaaat
    15481 tgaaataagc aatcagttac ttataaatac atggaaaaaa cttttggaag gacaagcaca
    15541 cgatagtcta gctggatgtg taactgatac tgtaacagat tatattcttc accgtataag
    15601 agaagcaaat gaaatatgtg acagtattga aaatactatt gtaaaaaaga tttcagaagg
    15661 gcttaaatta tctaaaaatg atattttagt atttaataca gaagctaagc gttttaatgg
    15721 atataaagag atacaagttg taagtgatag taaaaatatt tattttaaag ataatttaga
    15781 tgctactata attgaagaaa cttatgtaaa accgagagaa aatgtattag aagaaacacc
    15841 tgctggcaac gtatttattg aagaatcagg atattatata ttaaaagtaa gaataaatgt
    15901 acagcttcct gcacttggtt ataaagttgt tagttttgaa tcaagtgata aagaaatggt
    15961 atcacttgat aaatctaatg acacttttat atctaatgat aattataaga taatctatga
    16021 agatggaagc ttaaatttac aacttaaaga tggaacttac atatcaaact ttttaatatt
    16081 aaaagatagt ggtaatgcag gcgataccta tgacttttct ccattaaaaa atgacgagga
    16141 tataaaactt tcatttgata gagtgtatac agaaaaaact ttaggatatg aaaagttggt
    16201 ggttaaaggt actactttac taccacttac attagaagat agaaaaaaca aatatcttaa
    16261 tgggaaatta aatgtaaaag taagtattgt cttatccaaa gaaaatccat tgatggatgt
    16321 aaaactttgt gttgataaca cgatatataa tcatagacta cgtgttcata taaaaactga
    16381 tataaaagat aataaaaaca tagcatcatt gccatttgga tatataacaa gagaaaatgg
    16441 agttttagac aactgggaag atatttacag tgaaatgcct atagatttag aacctttgga
    16501 aagtaatatt actctaacaa accaaaatag aagctgtact gtatttacta gaggaataaa
    16561 agagtatcaa catattgaag atgaaattgc attgacctta cttgctacta cagatgaact
    16621 aggaaagcct gatttattat atagaccagg acgtgcttct ggtgatacaa ctaaaaaagg
    16681 tcatataaga attaaaacag ataaagctca aatgcttaag aaattagaat tttcatttgc
    16741 aatatatatg gattctaaat catttgatga attggaaatt gcgaatttaa cccacaatta
    16801 tctaagggaa tgtgtaaatt atcaattaca ggattacaat ttctttttat atcgtataga
    16861 taataagatt caaaaatcaa ttgtcaataa aactgtaagt agagaattag aaataatatc
    16921 tttaccagaa aaatatctta tatcagcatg ttatccttct tattacagta aagataaatt
    16981 tataataaga gtggaaaatc caagtagtaa tagaatgata ttggatgagc aaatatttaa
    17041 aaatagaaat gggaaaattg taaatgcaat agaagatgta gaagaagaac aggtatatga
    17101 aatagcacct tttgacgtta tttcaatatt attggattta taagtgtaaa agtaagttta
    17161 ttgtttaaag tctgacagat attaatctgc cagacttttt aatttgtata aatttgggta
    17221 aatattaatg aatagtttta gtatttaaat atgaatttag tatttaatat agaatttatt
    17281 gaagtagaat aattagttta acaggataag cagtttaaat aaagaattat tatattagag
    17341 tgattttata tataatttaa aataataaaa tgttaataaa atgagttaaa gattgtatat
    17401 taatagggtt gatgtagtta caaaaaagtt accaattgtt atgtatttac aaaactgata
    17461 tattaattac ataaaatcta aaatagtttg taataggagt atatataata aaaattttaa
    17521 ttaaataaag ctaggtatat ttataatatt aataaatata ttgctataaa agtttgttta
    17581 ataaattaaa taaagaaaag aggagaataa aatgtacaac atagatgata tgatatataa
    17641 ttgtccagta gaagctttat caaatatttt aggtaaaaaa tgggtggcta taattatatg
    17701 gaaaatacaa gaagataaga aacgatttgg agagctacaa agagctatac cagactgtag
    17761 taagaaaatg ttagttcaac aactagattt tttaattgaa caggggatta tagtcaacca
    17821 aaaaaagttg gttaataatg ttgtagagtc aacatatttt ttaagtgaat caggattacg
    17881 attgcttcct gtcatggaaa aaatgattat gtggagcaat aaaaatttaa attgtgataa
    17941 atactaaaat ttactctgta agtaatgaaa atttaattaa gctatgagta gaaaaatgta
    18001 ttaaaattac ataatttttt agttagattt taatttagaa atgggattgt aatattggaa
    18061 ataaaataag gaggaatttt aatttgcaaa atgtaaattt atttttagta tttatagaag
    18121 gtatagtatc attcttttca ccatgtatac tgccaatttt gcctatttat ttgagtatat
    18181 tgtcaaatag tagtgtagaa aatctaaaag aaggaaagac gagttttata ggaagttctt
    18241 tatttaaaaa tactatattt tttgctcttg gaatctcaac aacattcttt atattgggtt
    18301 catcagtaaa agtattgagt atgtttttta atgaaaataa agatttaata atgtttattg
    18361 gtgggattat aattattatc atgggattat tttacatggg aataattaaa tcttcaatat
    18421 tgaatagaga aaaaagattt aatgttaagt ttaaagaaat gaaagcaata acagctttta
    18481 tattaggttt tacgtttagt tttggatgga caccttgtat tggtccaata ttggcatctg
    18541 tattagtcat ggtttcaagt tcaagtaatc atcttagtgc caatctttta atagcagtat
    18601 atactattgg ttttattttg ccatttataa ttacagctat gttttacagt aaactattta
    18661 aaactataga taagataaaa tctaacatgg aaattattaa aaaaataggt gggattatac
    18721 ttatagtatc aggaatatta atgatggtaa atggatttgg aagtataagc aaacacttta
    18781 atacgtcaca aaatagcaaa attgaaagca agcaagaaga aaataagcga gaaaattcta
    18841 ctgataaaga agaaaattct gatggtaata atagtcaaaa agactctaat aatgacaata
    18901 atgataacgg gtctaatgat gaagatagaa taaagtctat tgattttact ttaacagacc
    18961 agtatggtaa aacacataaa ttaagtgatt atgaaggcaa agttgtattc ttaaatttct
    19021 gggctacatg gtgtcctcca tgtaaagaag aaatgcctta tatagaacaa ctgtataaag
    19081 actataataa aaacaatgat gatgtagtaa ttttaggagt agcttcacct aatttaggta
    19141 gagaaggttc aagggaacat gtagttaact tcttaaaaga ccaaggttat acattccctg
    19201 ttgtacttga tgaagatgga gcattagcat atcaatatgg aattaatgct tttccaacta
    19261 catttataat tgacaaggaa ggatatgtaa ctcagtatat tccaggagct atggataagg
    19321 caactatggc atcatttatt gaaaatcaga gaaataaata aaagtattta agtatatgtt
    19381 tatgaatcaa tttaaggtta caaaaaagtg cctaattgta ccacctaaat aatcttgtta
    19441 tagtaaaagt ataaacaata caagaaaggg ttgaatttta tatgaaaaaa acatttgatg
    19501 ctataataat aggatttgga aaaggtggaa aaacattagc aggagatttg gcaaacaggg
    19561 gattaaaagt agctttaata gaaaagtcaa acaagatgta tggtgggaca tgtgtaaatg
    19621 tggcatgtat accaacaaaa agcttagaaa attcagcaaa cagcgtaaaa acaaagaata
    19681 taaattcttg ggatgaagta caagctgaat atgagaaagc tatagataaa aaagaaactt
    19741 taattacaaa actcagagaa gctaactata ataaattaaa ttcaaatgag aatgtaacta
    19801 tattcacagg aatgggaact ttcattgatg aaaaaacagt tcaagttaaa actgaaaatg
    19861 aaatttatga gttagtagca gataatatat ttattaatac tggttcaaga ccatttatac
    19921 ctaatattaa gggaattgaa aataagaata tagtatatga tagtgagtct cttatgaatc
    19981 taagagtttt acctaaaaaa atgacaatta taggagctgg atttataggt ttagagtttg
    20041 ctggtattta tagttcattt ggagcagaag ttacaatatt aaactctaat aatggcattt
    20101 taccaaatga agatgttgag gattcagaag aaataataaa attacttgca aagagaaatg
    20161 taaaaattgt taataatgca aatataaaag agattaaaga agtttctgaa tcagcaatag
    20221 tagaatatga agtagatggt aaatctaaag aattaacaag taatatgata cttgtagcta
    20281 caggaagaaa agcaaacact gaagggctag ggctagaaaa tgcaggtata gaattaaatg
    20341 aaagaggatt tattaaagtc tcagaaactc ttaagactaa taaagagcat atatgggcta
    20401 taggagatat aaatggaggt ccacaattta cttatatatc attggatgat tatcgcatag
    20461 ttattaatca gttatttgga gataagacta gaacaactaa tgatagaaag aatatcccaa
    20521 attctatatt tataagtcca gcattttcaa gagttggtct taatgttaaa caagctaaag
    20581 aaaagggata tgaagtatta gtggcaaaga tgccagtaga agcaattcca agagctaaac
    20641 agattggcaa ggcagatggg tttataaaaa tagtgataga taaaaaatcc aataagatac
    20701 ttggagctag tatgatatgt gaaaattcaa gtgagattat ccatcttatt caattagctg
    20761 tagatttaga agtagagtat acatatttaa gagaccgtgt ttatgcacat ccaactatga
    20821 cagaagctct taatgatatt ctatctccta atatgataaa agaagtataa cataagttta
    20881 atatataaac ctaaaattat cattataagt agattttaga aagaggctat ctcaaaaaat
    20941 agagataagt ctctttttta ctttttaaat gataaaaata tatttgattt tataaaagaa
    21001 gattatctta tgaactaaaa agtttatttt aaaacactac ctatttatgt tataaactta
    21061 taaatataat atagaatcta ttattctgta ttattaagtg aggttaagcg ttataattaa
    21121 ttataaatat tgaataatct aaatggataa gataatggta ttaaaaatta tcttaaaagg
    21181 tgaaataatc tgtatttaat gtagtacagt tatatcttta aagcaaggtg gtgaaatgat
    21241 ataaataaga tatctaaaag agggtttgat gatagtgata aaagatggtt ttcaagtaaa
    21301 gaattaacaa gacttataaa ggctaaagaa gaaatagaat ggttattaaa tagggattac
    21361 aaaatagacc cagtcgttac ttttgttggt gatagatatc agttttctat aagacaaaga
    21421 gatgcattaa aaagagcagt ctgtactgat gaaaaaaata ttataagaca atcaaaaagg
    21481 ctttttctag ataaaatcaa agaagatacc gtaaatatag atggctttaa tttgataata
    21541 tccatagagg tagcattatc tggtggtact ctagtaatag gaagtgatgg aaatataaga
    21601 gatttagcag gtcttagggg aacctataag attatagata aaacagaaga agctttgaat
    21661 ctgataggta aattttttaa taaatacaat gcacagaatc ttaaatttta tttagatgct
    21721 ccagtttcta attcaggaaa tttaaagtat aggatattag agcatgccaa aacttgggga
    21781 attgaaactg aagtagaatt agtaaaaaat gcagatgttg tattggaaaa attagataga
    21841 gtagttagta gtgatgcagt catagttgac aagtgtataa gttattttaa tgtggctaga
    21901 ggtataattg aagagtatat taaagaatgt aatatcataa atttaaataa atagataaat
    21961 agaggtgaga atatataagt atatgtagca atattattag taatacaagt aaagtttgaa
    22021 aataggagaa cgaatacata tcgaatttca taaaatgttg taaaaagaca tatgagagga
    22081 tggatatgtg tgaaattaac taaactaaat attaaaaaat atagagcacc tatatataaa
    22141 tatgctttag caaatgtaaa tttgaggtct gctaaatcta ctaattcaag tatcataaca
    22201 gtaatacctc agggagctaa aatggaagta ttggatgaag aagatgactg gattaaggtg
    22261 atgtacaatt ctcaagaagg atatgtgtat aaggatttgg tatcagtaag cgaatatgct
    22321 tggagtaact taaacttaag agaagataaa tctataactt ccaatataat tacagtaatt
    22381 cctgaaaaat ctagggttga agtccttcaa gtagatggag attggagtaa gattgtatat
    22441 gatgataaaa taggatatgt ttttaattat tttttatcaa ctgatgggaa taaaccaaat
    22501 gaattagatt ataagtattt ttatactgac atgattaaat ttgttaacga aaataatatt
    22561 aaaagtacta gcgattatct aattgtaaca gaccttagaa ataaatatac ttatattttt
    22621 aagaaagata atggaggctg ggggcaactt tacaagtggc aatgtactat aggcaagcct
    22681 gagactccaa ctataacagg tatattttat ataagtggaa gaaaaccttc ttttggaacg
    22741 gatgagtatt cagttaagta tgctactaga attaaaggtg gatattacta tcattctgtg
    22801 ctatatgatt caacaggaag ttatataata gatggcagac ttggtgaagc tcttagtcat
    22861 ggatgtataa gattaagtac ggaaaatgca aagtggatat atgataatat accagataca
    22921 acaacagtta taattcatta agaaaaattt tgagttttta ttgggataat gattctcaaa
    22981 ttaataggat tatatattaa cttcttcata aagagtacta tttaaaaagt gtaataagag
    23041 agtgctatta acaaaacaaa gatattttga ggcactctct tataaatata cataattaag
    23101 atattttcat aaacaattct ataatcttct ccttatcaaa gaaaacagga ttagcaccag
    23161 cacaagcatc tttcatagag ttttctgcaa gcaattcaaa attaggatta tcaacaccta
    23221 catcttttaa agattttggg attccaacct cttcagaaag agcttttatc ttatctatta
    23281 caaagtcaac acactcttta tctgatttat tatttacatc cattccaata acttcagcta
    23341 ttggtcggaa tttggcaggg gctgatttag cattttcctc ctcaactatt ggaagtagca
    23401 tggcattaca tactccatga ggtaaatcat ataaacctcc taattgatga gccatagcat
    23461 gtacatttcc aagacctgca ttactaaagg caattccatt taaaaaacaa gcataacaca
    23521 tttgttctct agcttctatg tcattaccat ttttaacagc tcttggaagg tatttaaata
    23581 tctgctttat agcatataag gcagttgaat ctgtaacatc ataagcacca ttagctacta
    23641 cagcttctac agcatgagtt agtgcatcca taccagttgc tgctgttaaa ttagatggtt
    23701 tgctaatcat taattctggg tcatttactg tcattgtagc tagtgagttg gtatcaacca
    23761 ttatcatctt tacatgtctg tcttcatcag ttataacata gtttattgta acttcagcag
    23821 atgtaccagc agtagtagtt atagctacga taggaagaca ttttttagaa gttttgttga
    23881 taccttcata gtctctaata tctcctccat tagtagctaa tatagagata gctttaccac
    23941 agtcttgtgg agaacctcct cctatagtta taacgaagtc acaattttct gattttaata
    24001 ttttaagtcc tttatccaca ttagttacgg ttggattttg tttaacttca tcataaacaa
    24061 caaagtctac atctatctga gataaaatat ctgttacttt ttttacagta ccatttgaaa
    24121 ctagaaattt atcactaaca acaaatgctt ttttagttcc taatgattta attgaatctt
    24181 tagcttcatt aagacatcct ttcccaagta aatttattgg tggtacataa taagtgctca
    24241 taactaaaat ctccttttat tttcattgat ttttatgtaa aaatatatat ctaacaattt
    24301 tcactaattt actcataata tatcgtatct tagattgaga tagggttact ggttcttata
    24361 taagaactta attcgtaatt acgattatga ttagaattta acatagtatt tttatcatgt
    24421 caataaaaat ataaaaaata ttataaaaaa aacaaagtta ctgtagttaa atcagcaact
    24481 ttgtttttat agtataaatc aaataaatac attagtttaa aacaaaattt ttaattatta
    24541 tgttttgata tcttccatat acagaatatt aatctaattt attacaatgt cttcattacg
    24601 ttcttccatc attgattgaa tatttttctg agcatttaag agtaattttt ttatcagttc
    24661 tattttttcg tcattagctc taaataaagg cagtgttaca ctaacagcag ctcttataca
    24721 tccatttttt cttattggta cggcaagaca ttttaggtgt tccattgatt cttcacactc
    24781 tgaagctatt tgtgttcttt gaacttcaag aagctggtcg tataaaacat caaaatctgt
    24841 aattgatttt tctgtaaaac cttttagacc atctggatat aagcttatta gtttttcctt
    24901 gtcaaaatca cataaaagag cttttccaag acctgtacaa ttagcaggta ttttttttcc
    24961 aacataagaa ataagtctaa ttggttctgg agaatctact tttccaatat aaagaacttc
    25021 attattgtca agtataccaa gctgacaagt ttcagaacag gaattaacta tattagtcat
    25081 ttcgtcaatt atatagttaa aaatagttct tttttcaaaa aatgaagacc ctaatgtgta
    25141 agaacgtatc cctatagaat atttggatgt gtttttatca atagtaatgt attttctagc
    25201 attaagtgta tgaactatag gaaagatgct actttttgga gcattgataa catctgctat
    25261 ttctgtaaga gtatatccat tttcggattc tgctaatgct tctaaaatat ctaatatcct
    25321 agctgttggg cgatgattat ctgaagtcat aaatttcccc ctctttttat taatttaaag
    25381 tattagaata tgttgatttt agcacactat acgatatttt acaatgtaga aaataaatat
    25441 gtaaatttat atataaataa gctcttgatt tagatacaga tagatatttt tttagactgt
    25501 acattaagga ataactaatt tccaaaatat acctaaagaa agtaagtgat atttagttat
    25561 ataatttaag taaatatatg caatagttat ttttatatgt aaatatcaaa atattatgaa
    25621 tttatatttt atctattttt tacactgtat aaaaacttta tagttttgaa tactatataa
    25681 ttttaaatac gctgtatttt tgcatataag gaagtcattt gataatatac aaggtattat
    25741 cagaaagttt gaaaaaaatt tgacaaaaat ttattttttt tattgactca taaataggaa
    25801 aatgctatca ttaattcaaa gattcgtaat taaaaactta gttcttaaat acgaaccgaa
    25861 acggccgtta gaattaaagc taaaaactta ataagagtaa ttaaatatca tgttttagga
    25921 ggattaactt tatgataatg gattttatta aaaaaattcc tgcaggtatg atgatagtgc
    25981 caatgtttat agcagctttc attaatacct tttgcccaga tattgtacaa ataggttcgt
    26041 ttacgacagc agtattttca agtgttgggt ctgcaactat aattggggtt caactggtat
    26101 gcttgggaac tcagcttcaa tttagagaaa tgccaaaagt tttaaagaga ggtggtatac
    26161 ttcttggttc taaatttgct ataggtgctt tgataggtat acttatagga aagctatttg
    26221 gaatggatgg tgtccttggt cttacaacat tggctgttat aagtgcagtt acaaatagta
    26281 atggtagtat atatctttca ttgatgactt catatggaga tgaaacagat tgtgcaacta
    26341 tgagtttact tactttaaat gatggaccat tttttacatt aatagctctt gggacttcag
    26401 gtcttgcaaa tgtgccattg ttgtcattat tagctgcaat agtgccaata cttgttggta
    26461 tgataatagg aaattttgac aaaaaaatga aagagttttt agagccaggg tgtaatttac
    26521 tcgtaccttt tgtagggttt gcacttggta gtagcataaa tcttgcaagt atactaaaag
    26581 gaggtttatc aggagtatta cttggattaa tatctgtatt tgtaggaggt atatttatag
    26641 tattctgtga taaatttata ggaaaacgtc caggatatgc aggatgggct gtagctacaa
    26701 ccgctggaaa tgcaattgct gtacctgctg ctgttgcatt agttgaccca tcatggcaac
    26761 catatgttgc aacagctaca acacaagtgg cagccgcagt agttgtcact gcaatattag
    26821 tacctcttat aactagttgg tgggctaaga agtatggatg tcctaagttc ccaaaacaaa
    26881 atgaggatgg tacaataaca gcttaattta tattacagtg agttgttggt aaaataataa
    26941 attctttaat tagattttag gatattaata aaattatatt atttataaag gagagcaaga
    27001 ataatgataa atgcagtgat aattgatgaa aaagataatg ttgcagtagc tattgaagca
    27061 atagctaaag gtaatgaaat tagttttaaa ttaaaagaca aaacagtaaa aacgattaca
    27121 gcactagata atataactat ttatcataaa ttggctataa aagatatggc ccaaggtgat
    27181 aaagttacta aatatggtga acatatagga gaagcaaatc aggaaataaa aagtggtcaa
    27241 catgtacata ttcataatgt tagaagtgtt agagaggatt tggataaaca agttatgact
    27301 aatatataaa ataatattta tagagttata aaagttgtat tcattgcttt tagtgtcaaa
    27361 tagtttttaa ataggatttt agctgtttga ttagtgagtg taataaaaat tataatatat
    27421 aaaaattata aaaagtatat agactatttt aggaggaaat cagtatgaaa ttttatggat
    27481 ataaaagacc agatggaaga gttggtataa gaaaccacat attaatattg cctacgagtg
    27541 tgtgtgcatc agatacaact agaataatag catcacaagt tcaaggggca gttaccttta
    27601 ataatcagaa tggatgttca caagtacatt cagaccaaca attaactatg gatgttatgg
    27661 ctggttatgc agcaaatcca aatgtatatg gtgtaatagt tgtatctttg ggatgcgaaa
    27721 attgccaaaa tgatttggta gttgatgcaa taaaagagag aacaaataag ccaataaaaa
    27781 ctcttgtaat acaagaagaa catggaactt taaaaactat agaaaaagct gttcgttatg
    27841 ctagagaaat ggcacaagaa gcttctttgc ttagaaaaga agaatttcca atatctgaac
    27901 ttatactagg aactgaatgt ggtgggtctg acccaacaag tggattagca gcaaatccat
    27961 taataggtga attaagtgat aaacttgtcg atttaggagc aacatcaatt ttaagtgaaa
    28021 ctactgaatt tataggtgca gagcatatac tagctaggag agcagtaaat gaagaagtaa
    28081 aagaaaaaat attacatata gtacatagat atgaaaattc attaaaattg gttggagaag
    28141 aagttagaga aggtaatcca tctcctggaa atatagctgg tggtttaaca agtttagaag
    28201 aaaaatcact tggttgtata cataaaggag gtcatagaca aataagtgaa gtatatgatt
    28261 atgccaaaca aattgataag aaaggtcttg ttataatgga tacccctgga aatgatgctt
    28321 catctgttgc tggtatggta gcaggaggag cacaaatagt ggtatttagt acaggaagag
    28381 gaacaccatc aggaaatcca atagcaccag taataaaaat aactggaaat aaaataactt
    28441 ttgcaaatat ggaagataat atggattttg atgctagtcc agttatctat ggtccacaaa
    28501 caatggaaga attaacagat gatttgttaa atatggttgt agatgtagca aatggaaaac
    28561 aatcaaaagc agagtccctt ggatatactg aaatggcaat agctagagta tgcaactatg
    28621 tataatcttt aagtaagaaa ataaatacat agaattttaa taaagggtga gattatgaag
    28681 tttgtaagtt ttactgaagg agaaaatgaa tgtaaaagaa ctggagtttt ttctaaagat
    28741 gaattattta ttatagatgt taactcttta aatctcagta gaaattttaa ggatttaaat
    28801 gaattaattc aaaatgtatc tagtgaagat attattaagt tgaaaactat tataaatgaa
    28861 agtatattct ataattatga tgtttttaaa agagagagat gtactgtaca tgttcctata
    28921 gaaaaaccaa tacatgatat cttatgtgtg ggagtgaact ataaagacca tcttgaagaa
    28981 actcaaactc actttgatga tagttttgaa gagccacaaa aaacggttta ttttacaaaa
    29041 cgtgtgacaa aggcaatagg tccagaagat gagatagatg gtcattttga gataaataat
    29101 caactagact atgaagttga acttggagta gtaataggta aaacaggtgt taatattaga
    29161 aaagaagagg tagaagatta tatctttggt tacactgtta taaatgatat ttctgctaga
    29221 aagttgcaaa aagaacatgt tcaatggttt agaggaaaag gattagatac atttacctct
    29281 ttaggtccat gtattttaca taaaagtgca gttccttttc ccataaaact taaagtatgt
    29341 agtagggtaa atggtgaaga aagacaaagt tctaatacag gattattcat atcagatgtt
    29401 tcagacatag tttcagaact ttcaaaaggt atgactttag aggctggaga cattatagct
    29461 actggaacac cttctggagt agggatggga ttttcacctc ctcgatatat gagtagtgga
    29521 gatgtagttg aatgtgagat tgagagtatt ggaattttaa aaaattacat taagtaaatg
    29581 ttttaatata attaatatta aagctaaatc tgatgtatta atgaacatat taaaataaat
    29641 tttaggagtt taaattatga aagcagaatt tttgacacca gttgttacaa tatttgatga
    29701 agaaggaaat ttagataaag aaggtaataa aaaagtgtac gaccatctta tagatggagg
    29761 tgtagatgga ctagtcataa tgggaagtac tggagagttc tttaatatgt ctatggatat
    29821 gcaaaaagaa cttatagatt tagtaactag tcatgtaaat aagagagtaa aagtgtttat
    29881 aggtacaagt cgtatgtgta taaatgaatc tatagaactt tcaaactatg cacataatag
    29941 tggagcagat ggcgttatga taataagccc ttattatttc tctcttgatg atgatagtat
    30001 tgagttattt tatagtgaaa tagctaagaa tacagaggct gaaatatatt tatacaatta
    30061 tccagataga actggttatg atttaagtcc agaacttaca ttaagacttg caagaaaaca
    30121 taaaaacata gttggatata aggatacagt aactttgatg ggacatacca gagatttaat
    30181 aaatactatg aaaatagagt tcccagaatt taaggtttat agtggttatg atgaaaactt
    30241 tatacataat ctaatgtctg ggggagcagg ttgtataggg ggattatcaa atttaattcc
    30301 agaagtttgc tccaaatggg ctgaagctta caataataga gatatggaga aggttataga
    30361 aattcaaaaa tatattaata aagcaatgga tttttactta ataaccaatc catttatacc
    30421 agcaatgaaa aaagctatga atatcagagg gttacaaata agtgattata tgactgctcc
    30481 atttgtaaaa ccaaatgaaa aacaagtatg tgaaattaaa aaattgatga ataaattaaa
    30541 tatatgttaa atcaaccttg caaagaggtc aacaataaat tacaattatc ttaataaaaa
    30601 ttaaattaaa ataatactag ggtactatat tagtttaata gggaaagtga tgaaaattca
    30661 ctacagcccc cgctactgta tacggatacg aaatcaaaat ccactgaaat ttataaaaat
    30721 tttgggaagg gtgatggagg aaaagccgtg agtcaggaga cctgcccagt attatataag
    30781 ttcaatttag ggtggagatt gtaatttata ataaaatgaa gtatcaattc gtttttgtaa
    30841 agttgatata tgaacttctc cttattaaat taggagaagt tttttatttt attaaaaggt
    30901 tatacttatt aaagatgact ttaggataac tttaataagt atactttaaa aagcatatat
    30961 tattaaagtt ttttagccac tcataaaaaa taagatattg gagtgaaaga gatgaaattt
    31021 aaatcaaatg ttttaaagca gtttttttta attgctatgg taatttttat ggtaggaggt
    31081 cttatagggt gtagtaaaag tgacaaagag aataaacaag ctagtactgg taagaaatta
    31141 gaattaaaat atgctagtgg atttagtgtt gaagatttag aagatggaat taaaaaagta
    31201 actgatggag ataatcgaga actgatttta atcccaaaga agattaaaat tcctgaaaaa
    31261 tataaaaatg ctaatatagt tagaacgcct gtggacaatg tattaatagc atctacaaca
    31321 caggcttgtt ctctgagaac aatagatgaa ctagatagta taaaagccgt aacaacagaa
    31381 gagcagtact ggactattaa agaaattaaa gatttaatga aagatggaaa aatacattac
    31441 gttggttcaa atttagcacc agattatgaa aaaatagtag acttaaatcc tgacttaact
    31501 atagtgagta gtggtttatc agaggctgat acaaaattta ttgacaaatt aaaagaatta
    31561 gggcttaatt atgttgtaga caatgagtat aaagagcaaa atccatttgg aagaatggag
    31621 tggacaagtt taatagctgc attttatgat aaagaagata tggctactag tgaactaaat
    31681 aaaactgtac aaaaggtaga agaagtaagt aaaaaaataa aaaacaaagc taaacctaaa
    31741 gttgtatggg catctgtata tgaaggaagt gcttatatag ctcctaaaga ttcatatgtg
    31801 gataatatga taaaaatggc tggtggaata aatgcatgtg cttcaataga ttcaaatgaa
    31861 gggcgagtaa gtattgaaca attgcatgaa gttgcaaaag atgcagatat atttgtatac
    31921 tcatcaagtt cagattatgc accaaattta gattctataa aatctagtgc acctgtattg
    31981 gctgatttgg atgttgtaaa aaacggaaac ctatgggtgt ttgctccaga ttattatcaa
    32041 tcagtagata agacggacga attaatagta gatttagttg agatgttcca cccaggtact
    32101 acaggtgaaa aaatcaaaca ttatataaat tatgataaat agtacagaga ggacacaaaa
    32161 agataggttt tatagatttt agttaatata actttgctaa gaggagtata caaatggatt
    32221 ttatcaataa aatgagaata aggaatacga caatatttat agtattaatt ataagtatga
    32281 tacttacttt tttagtgtgt gtagcatttg gctctgtaaa aattgaattt aaagaagtaa
    32341 tagatgttgt attaagaggt atggataata cagaacatta taaaataata tacaatataa
    32401 gaatccctag agtacttgca accatggtag gtggagcatg cctagcagta tcaggtattt
    32461 tattacagat attttttaaa aacccaatag ttgaaccgta tatattagga gtatcttcag
    32521 gttctacttt atcagtagct ctcatgattt tgggaggata cacattggga tttgagagtg
    32581 taaattcaat gagtatattt gtagcagctt ttataggagc tttaattgta atgtctatag
    32641 tcattttatt tgcaaataag gtaaagaata taacaactct tttaataata ggaattatga
    32701 ccggatatgt atgtagtggt ataacaaatg tgttacaatc atttgcaaat agtgataaat
    32761 taaaagaatt tactatgtgg tctatgggaa gtttttctgg atttacatgg gaaaaggtag
    32821 aaatacttat aatagttggt atattaggtc ttttgatgac tgtattcata ataaaacctt
    32881 taaatgcttt tttattagga gaagagtatg caaaaagtat tggcataaat ataagatttt
    32941 ttagagtttt gatagtgttt atatctagta tattagcagc aatagtatca gcatttgcag
    33001 gacctatagg gtttattgga cttgcagtgc cacaaatttc aaaattgatt aaaaaatagc
    33061 tacgcacact t
//
LOCUS       DAGQWR010000026        33053 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.026, whole genome shotgun sequence.
ACCESSION   DAGQWR010000026 DAGQWR010000000
VERSION     DAGQWR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33053
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.026"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(37..2703)
                     /locus_tag="MIW95_001082"
     CDS             complement(37..2703)
                     /locus_tag="MIW95_001082"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBY3150427.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKAHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKVKKEKFEEMIKSVEERLTNLESKIK"
     misc_binding    complement(2779..3041)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3342..4169)
                     /locus_tag="MIW95_001083"
     CDS             complement(3342..4169)
                     /locus_tag="MIW95_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY3150428.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(4217..5653)
                     /locus_tag="MIW95_001084"
     CDS             complement(4217..5653)
                     /locus_tag="MIW95_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBY3150429.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(5740..6609)
                     /locus_tag="MIW95_001085"
     CDS             complement(5740..6609)
                     /locus_tag="MIW95_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBY3150430.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGNYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(6767..7420)
                     /gene="phoU"
                     /locus_tag="MIW95_001086"
     CDS             complement(6767..7420)
                     /gene="phoU"
                     /locus_tag="MIW95_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBY3150431.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(7448..8212)
                     /gene="pstB"
                     /locus_tag="MIW95_001087"
     CDS             complement(7448..8212)
                     /gene="pstB"
                     /locus_tag="MIW95_001087"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBY3150432.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(8231..9055)
                     /gene="pstA"
                     /locus_tag="MIW95_001088"
     CDS             complement(8231..9055)
                     /gene="pstA"
                     /locus_tag="MIW95_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBY3150433.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVIIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(9057..9974)
                     /gene="pstC"
                     /locus_tag="MIW95_001089"
     CDS             complement(9057..9974)
                     /gene="pstC"
                     /locus_tag="MIW95_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBY3150434.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(10356..11591)
                     /locus_tag="MIW95_001090"
     CDS             complement(10356..11591)
                     /locus_tag="MIW95_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3150435.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DNNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDIYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAITAPTF
                     SGEYQTQSDSILRCTKHGHTRLAFTKILALFSIFIVMFVVCISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGLKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            complement(11592..12479)
                     /locus_tag="MIW95_001091"
     CDS             complement(11592..12479)
                     /locus_tag="MIW95_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3150436.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYIMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            complement(12624..13739)
                     /locus_tag="MIW95_001092"
     CDS             complement(12624..13739)
                     /locus_tag="MIW95_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3150437.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPNMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            complement(13775..14473)
                     /gene="vanR"
                     /locus_tag="MIW95_001093"
     CDS             complement(13775..14473)
                     /gene="vanR"
                     /locus_tag="MIW95_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBY3150438.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            complement(14855..15535)
                     /locus_tag="MIW95_001094"
     CDS             complement(14855..15535)
                     /locus_tag="MIW95_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3150439.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKEISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYMTYINEEQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(15585..17600)
                     /locus_tag="MIW95_001095"
     CDS             complement(15585..17600)
                     /locus_tag="MIW95_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBY3150440.1"
                     /translation="MKKYNILKGTLIVLIVSLLAIAGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGEDKLLSLELPEIFSASEVWINGEIVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDSSY
                     FMVVLCIICINGSISKIEEDKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFIIVILFSLMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KKFLADVAHDLKAPVSAIQSFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYILEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKVSDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(17672..18349)
                     /locus_tag="MIW95_001096"
     CDS             complement(17672..18349)
                     /locus_tag="MIW95_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3150441.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVERI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(18929..19012)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(19245..20087)
                     /locus_tag="MIW95_001097"
     CDS             complement(19245..20087)
                     /locus_tag="MIW95_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY3150442.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVMANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(20339..22123)
                     /gene="pepF"
                     /locus_tag="MIW95_001098"
     CDS             complement(20339..22123)
                     /gene="pepF"
                     /locus_tag="MIW95_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBY3150443.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEKYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(22189..22854)
                     /locus_tag="MIW95_001099"
     CDS             complement(22189..22854)
                     /locus_tag="MIW95_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBY3150444.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(23005..23166)
                     /locus_tag="MIW95_001100"
     CDS             complement(23005..23166)
                     /locus_tag="MIW95_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBY3150445.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(23244..23753)
                     /locus_tag="MIW95_001101"
     CDS             complement(23244..23753)
                     /locus_tag="MIW95_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBY3150446.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            23959..24177
                     /locus_tag="MIW95_001102"
     CDS             23959..24177
                     /locus_tag="MIW95_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBY3150447.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            24195..26141
                     /gene="feoB"
                     /locus_tag="MIW95_001103"
     CDS             24195..26141
                     /gene="feoB"
                     /locus_tag="MIW95_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBY3150448.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKSNTH"
     gene            complement(26301..27356)
                     /locus_tag="MIW95_001104"
     CDS             complement(26301..27356)
                     /locus_tag="MIW95_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBY3150449.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRANLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGTISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(27404..28204)
                     /locus_tag="MIW95_001105"
     CDS             complement(27404..28204)
                     /locus_tag="MIW95_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBY3150450.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     KVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFLIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(28197..28988)
                     /locus_tag="MIW95_001106"
     CDS             complement(28197..28988)
                     /locus_tag="MIW95_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY3150451.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMFDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTTLINAIPKVILDGLGVAGTLLPAVGFALLMDMLISKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAMNTLTEGEIDFE"
     gene            complement(29028..29450)
                     /locus_tag="MIW95_001107"
     CDS             complement(29028..29450)
                     /locus_tag="MIW95_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBY3150452.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLANIEYFCMTKDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSIFL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENRNKIKNVM"
     gene            complement(29466..29945)
                     /locus_tag="MIW95_001108"
     CDS             complement(29466..29945)
                     /locus_tag="MIW95_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3150453.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTRDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKEIGDLGIDIELRMLPRDKKKQLSDLIK"
     gene            complement(30077..32833)
                     /locus_tag="MIW95_001109"
     CDS             complement(30077..32833)
                     /locus_tag="MIW95_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3150454.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSKFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNTILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEIFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALESKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINGPMLFIDQISTISALEVGNLLMQGKNIEQIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLPNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT        10606 a         5825 c         3658 g        12964 t
ORIGIN      
        1 tatatacttt ttgtatgttt ttactaattc ttatatttat tttattttag attctaaatt
       61 tgttaatctt tcctcaacag atttaatcat ctcttcaaat ttttcttttt ttactttttc
      121 ttcttcaatt aaactttcag gagcttttgc taagaaacct tgatttgcaa gctttccatt
      181 aactctcttt atttcgcctt ctaacttttt cttttcttta gacaatctct caatttcttt
      241 ttcaaaatca actaattcat taagaggtat aaatatttca acaccatcaa taactactcc
      301 aactgcatct tctggaacat tagatttatc ttttgctatc tctacatttg aagcagatgc
      361 tagagttata aaatagtctt taccaagctc aatagcttct attttttctt cacttgggat
      421 tattatcaat ttagctttct tagaaggtgg tacattcatt tctgctctca catttcttat
      481 acttcttata ccttccatca ataagttcat catatcttct tcttcagcca tgttatcttc
      541 ttcattatat tttggccatt cagatactat tatacatcct tcaacagtag gaagatgagt
      601 atatatttcc tctgttataa atggcatgta tggatgtaat aattttaata ttttttcaag
      661 aacataagtt aatgtatata atgctgcaga tttagcattt gcatcttccc catataatct
      721 aggtttaacc atttcaatat accagtcaca gtattctgac caagtaaaat cgtatatctt
      781 ttgaagtgct attcctaaat caaacttatc catgttatta gttgcttctt ttacaatatt
      841 atttgctctt gatattatcc atttatcagc tagagttaag tcctctttaa cactttctct
      901 agtcatgttt ttaattatat cttcatcaat attcataaat acaaatcttg atgcattcca
      961 caacttattt gcaaagtttc tagcaaattc tactctttcc atatagaatc tcatatcatt
     1021 tcctggggaa tttccagttg ttaatgtaaa tcttagggca tcagctccat attgctcgat
     1081 tatctccaat gggtctatac catttcctaa agatttactc atttttctac cttgagaatc
     1141 tcttactaat ccatgaacca aaacatggtc aaatggcttt tcattcatac agaacatacc
     1201 tgcaaatgcc attctaacta cccagaagaa tattatatcg tatcctgtaa caagtacact
     1261 tgttggataa taataatcta aagcttcagt cttatttggc catcctagag tagagaaagg
     1321 ccataatgca gaactgaacc aagtatctaa ggcatcttca tcttgtttaa aatgagtgct
     1381 tccacattta ggacacttat ctggcatttt tctagcaact actatttctc cacactcttg
     1441 acaataataa gctggtattt gatgacccca ccataattgt cttgatatac accaatctct
     1501 tatattttct agccattgta aataagtttt atcaaattta tctggaacaa attctaattc
     1561 tcctttttta agaatatcta tagctggttt tgcaagttct tccatcttaa caaaccattg
     1621 ctctgataat ctaggctcaa ctactgtatg gcatctataa caagttccta cattatgatt
     1681 atgcgctttt atcttaatta ggtaaccttg ttcttttaag tctgctacga tagcttttct
     1741 acactcaaat ctatccatac cttcatattt tccacaaact tcattcatag ttccatcttc
     1801 attcatagta tttaactgct caagattatg tcttaatcct acttcaaaat cattagggtc
     1861 atgtgctgga gtcatcttaa ctgcaccagt tccaaactct aaatcaacat aactatctgc
     1921 aactattgga agttctcttc ctactaatgg aagtattgct gtttttccta ttaaatgttt
     1981 atatctatca tcttctggat ttactgctat accagtatct cctatcattg tctcaggtct
     2041 agttgtagct atttctaaaa agtcttcact atcttttagt gggtacttta tatgataaaa
     2101 gtttccgtcg tgttcttcat gttcaacttc tgcatctgat aaagtagttt tgcaatctgg
     2161 gcaccagttt attattctat ttcctctgta tatgtgacct ttttcatata ggcttacaaa
     2221 aaattctata actgcttcat tacacccttc atccatagta aatctttctt tatcccaatc
     2281 acaagaatca cctagttgtt taagctgatt agatattttt cctccaaatt catctttcca
     2341 ttcccaggct ctctttaaaa attcttctct acctatttca tactttgtct tgccttcttg
     2401 tttttttatt ctttcaacaa ctttaacttc tgttgctata gaagcatggt cagttcctgg
     2461 aagccaaaaa gcttcatatc catccatacg tttccatctg attaatatat cttgaagtgt
     2521 atggtcaaga gcgtgtccca tatgcaattg ccctgttata tttggtggtg gcatcattat
     2581 tgtaaatggt tttttatctg gatttggttt agatttaaaa tacccttcat ccatccattt
     2641 tttatatagc ctagcctcaa aatcttttgg gttataagtt tttgataaat tcgtattttc
     2701 cattgtcctg cctcctgtat tttataaata aattttttaa atatctttag aaataaaaaa
     2761 gagctcttca tcctctttaa aaaggacgaa aagctcgcgt taccacctta attccaaagc
     2821 ttaaaataca ttaacatata attaaattat tagttaatgt tattttatca actttgactc
     2881 tcaaagaatt taacggttct aaccgtctaa acttacttat agttcagttt agaaactcca
     2941 aagctacatt cagtaaatct tatacttaga aaatcttaca gccagtgaat tttcctctct
     3001 tttagtagta tttacttact cctcttcttc tcagtcttta atattacata ttattatata
     3061 caaatattat tctctttgtc aattattttt attttatttt ttctgaattt tcttaattat
     3121 gtctattctc ttttatatag ttgttatatt ttaaaatatg tttttacagt tattctgtct
     3181 tataatgtca ataattgcaa gattatttta ttttaatttt aatgctttct cattaatttt
     3241 atattatatt atccttacag tatacctctt attcgtattt tttatattat atgttttatt
     3301 ttataaataa tttactttat cttttactat atttaaattt attataagtt ttcttgccag
     3361 aaatttttag gtgttatatt ttctgtaatt ggcaatattg tatatcctct ctcttttaag
     3421 attcttataa tattatttaa agcatttaaa ctttgctctt tctcatgaat taatactact
     3481 aattcatctc tttttcttcc ataatataat atattactta ctatctgact tgtagaagac
     3541 ttccaatcct gtgtatccaa attccagtcc caaattaaat aatcatttgc cataagttta
     3601 ttatatgaac cttctggcat atatggttta cttccgtatg gtaatcttat taattttgaa
     3661 ctctgaccag ttatttcttg tagagtatta ttgcatgtat taaattcccc aactgttact
     3721 tctgggcttt tatataactt atgtatatca tgacttacac tatgaaaacc tattgagttt
     3781 tcctcttcca agactctcct tacttcttcc ttgtgatttt tcatattttg attaagcata
     3841 aaaaatgtcg ctttgacatc attttctttt aaaatatcaa gtagtgcatt tgtatatttt
     3901 gatggaccat catctattgt tatgtatgct actttttcag aagtagcccc tggacctttt
     3961 gtttcttctt gagatgcttt gtatatgatt tctcctaaag tattactttg agaaagcagt
     4021 tccagtgtat tttcaccttt tgaacttata ttttcaactg gaacattatt aattttttcc
     4081 tccaataatg ttccagttaa aattaaaaca gctataagaa atatccttat aaatacatga
     4141 aaaacagcct tattatcttt aattttcatc ccccatatct cccttttata aaagcataag
     4201 tttaagactt aaaaagttat tttttatatt tattctcttt cttattactt tttatatctc
     4261 tactaaatat ttttttaacc ttttcttctt ctttttctcc taaagattct actcttttga
     4321 ttcccaaaga agttaaaaat tgtaatacat ttaaattatc aaatggagtt tccatattcg
     4381 tactttcact atattcatca acattcttag taccttcttc cacacttaaa atacgcttcg
     4441 gacctcctac acagcctcct acacacccca ttccttctat aaatgtagct tctatttctt
     4501 tatttagaac tttctttaga ccgtccatgc actcttttgt tccttcaaat gccttttctt
     4561 caaattttat atttttgtca attcttctga ctgataactt aactgcttta cttacacctc
     4621 ctgttctagc ataaactcta cctgaaaggg acgcctctac tctattactc tcatccatta
     4681 ttgctggatt taactctaat gcatcaaata tttcttgcaa ctctttaaat gtaagcacaa
     4741 aatcaactgc atcttttata tcttctaaca ttgcttcttt cttttttgcc atacaaggac
     4801 ctatgaatac aactttagca tctggattta aaatttttac agctctccca caagcaatca
     4861 taggagaaac ggatggtgat acattctcca atatctgagg aaaactattt tgtatcaaac
     4921 taacccaaat aggacagcaa caactagtta taaagcatcc ttcttcgtcc tttttcatat
     4981 gctcataata atcatatgct tcttttgcag taagcatatc tgctgccaaa gcaacttcta
     5041 tcatatcttc aaatcctatc ttttttaatg cagttcttaa tttaccaggg cttacatctt
     5101 tcccaaactg tcctacaaaa gctggtgcaa ctattgcata tacagggctc tttatatctt
     5161 ttaacaattt tatcattggt ataaattcta ttttatctga aattgcaccc aacttacatc
     5221 ttgatacaca atcaccacaa gcactacata catcttcctc attacaatta atatgcttac
     5281 ttcttatctt acatgactca tcttccttat tattttcatc acaatatttg caagcatctg
     5341 atattacaga tattattggt cttttgactt catctaaatt tacagcttca tgtagtgact
     5401 cttttaaatt tttatctgta tcttctgtca catccaatcc catagttacc ctaattaaat
     5461 ttaccatagt atctttatca aattttccac taaaataatt atttaaatta tcctttaaat
     5521 cttcatttaa aattttatta ggtaagtttt caatatcctc tatatcatca ttccacactg
     5581 cttttacaag ttcgccaaaa agcttcattt tatatatttc tagtatttta gaattgtcat
     5641 ttctagtatt cataaaaaat agtacctcca taaattaaac ttatatatgt ttaatattta
     5701 caaagatact attttttatt tattatttaa ctactattat taatatgatt tatttagctt
     5761 tctcaacaac tctatttcaa tcattatttt cattagatga tagttgaata cggggctttt
     5821 ttcttcatct atattccagt ctagcttatc cacattatat gttttcttaa ggtatttata
     5881 tgtatgctct ttcaaataaa gtttattttc aagtaactct atagactgca tatgaaaatg
     5941 tatttctcta catatcctta aggcattctc aattttttct atattagttt cactcaaact
     6001 ataatccaac tcatcaataa gttttgaata tatttcttct aatttttcta cttcttcttc
     6061 aaatttctct acatttaaat tttcttttct aagaatttta tcttcaataa gtaacattga
     6121 aagttcttct atctttttaa attcatctat aacactattt agataatttg gtctagccac
     6181 ataataatta acaagtactc caaacaaaac acctacagat gtatctaaca ctctatgtaa
     6241 agaataatac acagggtcac tatttcctac acctagatgt atagccagaa aggttacaca
     6301 ggcaactact actgacttta ttttaaacag attgcataaa tatattgagc acattactcc
     6361 tagaccacat aaaaaagggt ctccagcttt aatgatggca aatatgaatc ctattatacc
     6421 acctaaaata gtaccttcca ctctattgat tcctgtcttt aaagaacctt ttaccgtatc
     6481 ttgaacagaa attatacatg ctactagaga ataaaacata ttctctacta gatagttacc
     6541 aagaatcata cataaagtga cactaattcc tgtttttata gtcctcatac ccatcttttg
     6601 aagtttcaaa gttccacctc ctaaaaagca ccttatataa aatacgcatc ctcatattta
     6661 atatgttcct agtatatcac aattttttaa atttacccat taatcatatt tttttatcaa
     6721 ttctttctta atttagacaa taaaaaaact gctttaaagc agttttttat cctatctcaa
     6781 ccattttacc ctcaattgca aaaattatct tctcacatac atttgttata tggtctccaa
     6841 ttctttcaag gtatcttcct ataagtagaa gttttactcc ttggtctata gtatttgaat
     6901 tttcatgcat tatttttaaa caatcaattt gtacttgttc atacaatcca tctacaatat
     6961 catctttagt tccaacatca taagctatac ttgcatcaaa taagattaaa gcttctctag
     7021 tctgagacat catagactta cattcttttg ccatcttagg aatatctatc agctctttta
     7081 tataagcctc tccacctatc ttcaacattt ctctagctat atttgctgca taatctccta
     7141 ttctttccaa ctctatcgat atattaccta atgaataaat aaatcttaag tcccttgcca
     7201 ttggttgctt taatactatt agctctatac tcttatctct tatttcttct cttaatttat
     7261 ttatttcttc atcatcttct actacttttc ttgcaccctc taaatccttc tctatcatac
     7321 aagtcacaga cttttctatt gcttcttcac atttttccat cataagtact gtttgttctt
     7381 ttaaagtatc aatacttata tctaaactat tattgaccat tgtctaactc ctttatagtt
     7441 atatatttta accaaatcta cctgttatat agtcttctgt tcttttatct acaggagtgg
     7501 taaacatagt ttttgtatcg ctaaattcta taacctctcc atttaagaaa aaagctgttt
     7561 catctgatat acgagcagct tgttgcatat tatgtgtaac tatgactatt gtgtagtctt
     7621 tttttaaatc ctctataagt tcctctactt ttaatgttga aattgggtct aaagcagatg
     7681 taggttcatc cataagaata acttctggtc tcatagcaat tgctcttgct atacatatac
     7741 gctgttgttg accaccagaa agtcctagtg cagatgattt aagcctgtct ttaacctcat
     7801 cccaaatagc tgcacctttt aagctttctt ctacaatttt atctaattgc ttcttatctc
     7861 ttagtccatg tgtacgagga ccatatgcca cattatcata tatactcata ggaaatggat
     7921 ttggtttttg aaataccatt cctactttag tacgaagatt tataacatca tctgaggtat
     7981 atatgtcctc tccatcaaca gaaatgtttc ctttaattgt tacatcttcg attaagtcat
     8041 tcattctatt taacgttctt ataaaagttg attttccgca tccagatgga cctattaaag
     8101 cagtaacctt attctctttt atatccatat ttattttctt taatgcttgc ttatcaccat
     8161 aaaataaatc caaatctttt acactcattt ttatcttatc gattaattcc attattcaca
     8221 cctcttaaat ttaataatta gctttattta atttcttagc aacaaatctt gctagcatgt
     8281 ttagtgctaa tacaattact attaaaacaa ttcctatact ggctgctgtt gctatatctc
     8341 cactctcttt agtcagtaag tatgagtgta cagttaatgt tcttgcacta tcaaatattc
     8401 cacctggcat cttggctaca gtacctgctg taagtaatat tgcagctgat tctccaatta
     8461 tacgaccaac tgataagatt actccagata agattcctgg tattgcactt ggaagtacta
     8521 ccttatataa agtttgaaat tttgtagctc ctaatgccaa tgatgattct ctataagctt
     8581 gaggaactgt ttttaaagct tcttctgttg tacgaattat tactggtaat attattattg
     8641 caactgtaag tgaacctgca actatggaat atcccatttt taatgtaact acaaagaaaa
     8701 ttcctccaaa taatccatat actatagatg gtatccctga taaactttca gttgcaaatc
     8761 ttatagctgt aactgctttt ccttttttag catattcttg taaatatata gcagccaata
     8821 ttcctatagg agttgctaca actatagaaa ctactactgt atataatgtt gtaattatca
     8881 ttggaagtat accaccatca cctgatgctg aataattgct aaataagaaa ttaaaattca
     8941 tgttgccaat tccctttata aatataaatc ccactataat gactaatgat actattgtaa
     9001 atagtgctga tagatatact aatgacttca atagattctc tttgaacttt ctcatcttaa
     9061 tttaccgcct tatttgaaag tttatttaaa actaagttta aaattaatat aaatgtaaac
     9121 aatactactc cagttgcaaa taacatttct tgatgtgtac caaatgcgta tcccatttca
     9181 agtgctatat tagttgtaag tggtcttaca ctatctgtta ttgcagttgg cataactggt
     9241 gagtttcctg caacaagtat tactgccatt gtttcaccta aagctcttcc tactcctaat
     9301 actactgcag ctaatactcc agatttcgca gcaggtagta caactttgaa tattgtttct
     9361 atttttgatg caccaagtgc taatgaacct tctttgtatg cttttggtac tgctcttatt
     9421 gctgtttctg atacagatat tattgttggt aacatcatta ctgctaatac tattactatt
     9481 gctaacaaac tttgaccttt tggaagatta aatatacttt gtatagttgg aactatcact
     9541 gcaagaccaa atacaccata taatactgaa ggtatacctg ctaaaagttc aactgctggt
     9601 gacattattt ttgctaatct tttaggtgct acttctgcta taaatacaga ggttaatata
     9661 ccaacaggta ctcctatcac aagtgctcca agtgttgcaa atattgatgc tactatcata
     9721 gatgatattc caaatttatt ggcagaagga acccaatctg caccagttaa aaagtctata
     9781 aatgaatatc cttcaaatat aaatggtctt aagcctttat aaaatacgaa tcctattatc
     9841 aaaagtaaac ttgcaactgc tactaaagca ctaagtagaa aaatattttt tgctactttt
     9901 tcaattacgt attttgtctt attgccatta ttgtcatttt tttctaaacc tactaatcta
     9961 gattttacta tcataatacc ctcccattaa aatatactta tcgattttcg ttctgtattc
    10021 aaaatttaat tttttataat tcgtcttcat caaatttcct gtacttttat tctacttttt
    10081 ttaagttaaa tctatattag gattaagtta agcaagtgta aaattatatt tactttttta
    10141 acattaattt tacttttaaa actatatttg acatttactt tacaatatac tgttgtttct
    10201 ctatattaaa atagcagata tagataataa tacagtttat gtataattta ttacctatat
    10261 ctgctattaa gatttgttaa ctaaatttaa cttttatact acatatataa ttatcgtatc
    10321 ctctagttta tatcactatt atttctatca aattgttatg atacttgatg tttgcaatag
    10381 gtacgaatag ccaaaaatat aaaaataggt atttctatca ctgagaaaaa cagtataatg
    10441 tgtggtgtcc aaaaactcat acctcctaag tgtaagaaat taaatgatgt catttgatat
    10501 aataaactaa tcttaagacc aattccccct ggtggtaata ggtatccaat ccaactaaca
    10561 cctattccca taggaattaa gcaaatagaa aaagcaatca gcatagaagt aagagaatcc
    10621 ttacattttg cagataaaaa taatgttaaa ctaaccattg caagtattga aattagacca
    10681 ccaatagcta taagaatctg tgcttgtagt aaatttatat ttactagact aatgacagaa
    10741 aacaacattt gaaatgaggt tttcaaacat tcagtaccaa aagctaaatc agaaattgcc
    10801 aaatggatag aaatacatac aacaaacatt actataaata tagaaaatag agctaatatt
    10861 ttagtaaacg ctagacgagt atgtccatgt ttcgtacaac gaaggatgct atctgattgt
    10921 gtctgatact caccagaaaa agtaggtgca gtaattgcaa cacatataat taataatata
    10981 aaaatgtaaa atattatata atcgaaagca tctttagaat aacctgggta tatctcaaat
    11041 ggagtatcta cttttctata aagttcttga gctttcattt gtgcactgga agtttgtcgt
    11101 tcaatcttca taatatcatt taaatgagtt ttgcattgtt cataaaaaga atctaaatca
    11161 tttgtattta tttctgataa ttctgttgca aatcctgttt ctgaattagc aaatgcttca
    11221 ggaagtctat tcaacaatgg gcgtattggt aaaatacttt tagaataaat atctagtggg
    11281 aaatcttcac tacctacttt accatacttt tcttctgttt ttttccatat atttaaagca
    11341 tgctttaatt tttttggtgt tacaactcca ctactaattt cactattatt agcataaatt
    11401 gtttttttat atttaatagc atctaaacca ctcaattcaa ctaccttgcc attattatct
    11461 aaataattga taccctcgta agtaattgga agaaaagcca tcaatgcaga taaaaccaat
    11521 gccaacaata ataaaattaa tgtacgacgt gagtgcaaca ctcgtttaat ttccaaataa
    11581 aaaatacgca tttatttccc ctccttacta taaactgctt ctggaaataa ccataaatat
    11641 aaatcttcta gtcgtggttc aacagcaata gagttatcaa tttgtgactt ttctgccaag
    11701 taacgaatag atacttgatt gtttttttca ctacgctgat tgattatacg taaattcatt
    11761 tcatattttg aaatatctct atttgatata agacattccc atactttgcc ctctactttc
    11821 ttaaccagtt tatcggttgt tcccacatca agaatttttc cacttttcat aatagcattt
    11881 tgagttgata tgtactcaat atcagataca atgtgagtag atattaggac aacacgatta
    11941 tgagaaaatt ctgaaataaa attacgaaat ctcactcgct caccagggtc aagccctgct
    12001 gttggttcat ccattataag gattttaggg tcatttaaca tggcttgtgc aattcccact
    12061 cgtcgtttca ttccaccaga taatttaata atctttttat tttttacatc agataatgta
    12121 agaatttcca atagattatt aatttttcct tttacttctc ttggtggtac atctttcaat
    12181 gctgctatat attctaaata atcttttaca gaaaaatcct gtgggaaacc aaattcttgt
    12241 ggtaaaaatc ccagtaaatt tctgtactca gcacccatta tataaatatt ttttccatta
    12301 tacagaattt tgccattggt aggtttcata atgtctgtaa taattctcat caaggtagtt
    12361 ttacctgctc cgtttgctcc tagaaatccc cacacacctg gtgttaaggt aaggcaaaca
    12421 tcttctacag caattttatc cttaaatttt tttgtcacat gttcaatttt taattccata
    12481 tttgaaactc ctctctttat ctacttacct gcaaaaacaa aagtgtcttg cttagtattt
    12541 attttactaa acaaagacac tcataatttt aatattaaat agataatttc ttaagaaaat
    12601 cctaatattt gatttatatt ttactaaata gatgtaggaa gcgatatagt aaatgttgtg
    12661 acttcatctt tactttgaac agtaatatca ccaccatgca aatttataat ttccttagct
    12721 atagaaagcc ctaaacctgc accacccata tcagattttc tagcttcatc taaacgattg
    12781 aacttttcaa atatagcaga aagttgttct tctggaattg tctgtccata atttctaaaa
    12841 gcaatatcta ccttgctgtc tttatgaacc gctgagataa taatttctgt attaggatag
    12901 ctgtaagcaa tagcattttt caagatatta ttaaacactc ttgcaagttt ttcagaatct
    12961 gcttttattg ttaaactttc atttgctttt agaatggctc tatttccttt ttcagacagc
    13021 aatgggtaaa attcatcctt taattgaagt aacatataat ataggtctac tttttcgaaa
    13081 ttaattttga tttgattaga attatatcgt gttatttcaa aaagttcatt gataagtttt
    13141 tctaaatatt gtgctttgtc aagtgcaact tcagtatact tagatttttg agaattatcc
    13201 atattaggta tttcttttaa taagctcaaa tacccaataa ctgaagtaag tggtgtcctt
    13261 atatcatgtg ctagatacat cacaaggtta ttctttttat tttcagcctc tttcactgct
    13321 tgttgactta acaaaacaga catcttaatt tggttcattt gttgctctat ttcctttaat
    13381 ggcttagata gttcaaccaa ttcatcattt tgttgataaa tagtatgtgt tgcattgatg
    13441 atttctctta aataattcca tggttttttc caataaaaat tgaaaatagt gatatatcca
    13501 attataaaaa ccaaaaaaaa taatacatct cttctcaact taagccattt aacaagagga
    13561 tttgaaaaat ttgaaaacgt ataatcagcc ataaacatta aaaccaatac tatacctgta
    13621 tagatagcta acattatata taatttatac tttaaatacg cctctgtatt tttttctctt
    13681 tttataaatt tatatttatc tcttagtttc ctataagtaa aattattaat ctttttcata
    13741 atactacatc ccttcttgac aagaaaaata ggtgtcaaat cttgtaacca acaccccaaa
    13801 ttgtttttat atattttgct tcatctacag tatcacctaa tttttcacga agatggcgaa
    13861 tatgaactgt tatagtattg ttacttttat tgtaatattc atctttccag atagcatgaa
    13921 aaagattttc tgaatttacc acagagcctt tatttttcaa taatacatgt agaatagaaa
    13981 actctgttgg agtaagtgaa agagctttac catttaaatg gcattcatat gtttgggtat
    14041 tcaattccag acctaaaaaa gaaaaaatat gtgaaagttc ttcaccagat tgtaaacttc
    14101 cactgtattt cttatatctt ctaatctgtg ccttcactct agcaacaagt tccaaaggtc
    14161 ggaaaggttt agtaacataa tcatctgcac ctaaagttaa gcctgtaatc ttgtctgttt
    14221 cttcatcctt agctgttaac ataatgattg gataagtata tttttctcgg attttttggc
    14281 ataaagaaaa tccatttact cctggcaaca taacatctaa aattgcaagg tcaaattcat
    14341 ttaaattaat gcagtttagt gcatcaagac cagaataaaa tttataaaca atatagtttt
    14401 catttgtaag gtagacttct attaaatcag ctatatcacg ttcgtcatct acaactaaaa
    14461 tttttccact catagacttt aaacatcctt tctatatata tttatatata ttatactaaa
    14521 tttaattttt ttatatgatt ttttacatta gaatttatta agaaattctt aatcttatta
    14581 tgtaactatt ttattattta acatatatta aagataaaaa tgaatatata tagatttaga
    14641 tatacaattt atctatcttt tcaaatataa tctataaaat aatagttctg ttacgcattt
    14701 tttcatataa taaaactgac tgtaatttaa ctggcaaagt ctagaagaat aggctttatc
    14761 aattaaatta cagtcagcta ttttataata atttagttta gatatcgtta gcatttcaca
    14821 tttataacaa ttattaatga gattaatata tttattattc tttattaaaa taatatcctg
    14881 ctcctcttat tgttataatt ttctgaggat ttttataatc tttctctatt ttctttctta
    14941 atccattcac tgcaactacc acactatagt tatcctctaa gtactcattc atccatacat
    15001 gttcatatat ctctttagta gtcagagtct tttcaggatt ctctataaaa aagtttaaca
    15061 gcttattttc tgttggagtc aattttatta ttttgtcttc taactttatt actccactag
    15121 ttctattata ttcctctaat tttgtattat tttcattatt atttacatat gagttcattc
    15181 ttcttaaaat agcttttact tttgcatata atatttctaa atttagaggc tttattaagt
    15241 aatcatctcc gccagaattt aaagcattta aaatgctttg ttcttcattt atataagtca
    15301 tataaacaat tggacaagta gttacatttc taattttttt acaaagagtc catccatcac
    15361 catcagttag aattatttct aaaaatatta aatcatattt attgctaaat atttttccaa
    15421 tagcttcttc acaattatat gctaaatcta tacttatttc tttttcctcc agaaatcctt
    15481 ttagcttctg gcaattatcc ttgtctccat ctattatcaa tatcttagtt tgcattataa
    15541 tcactcccaa atatcattat tcttttatat taaataagct attattataa aaaacataga
    15601 cttaatttta ttgtaaaaac agttcctttt cctagttctg aatctacaaa gatttctccg
    15661 ccatgctctc ttataatgga tttgacaata tataacccta gcccttgccc ttttggctca
    15721 ttattatctc tttctgtaaa gaatctgtca aatacctttg gtatattttc tggaggtata
    15781 ccttttccat tatcagaaac tttaataatt gcaaatccat cttctttatc cattgataaa
    15841 attatctttc catcaaatgg agtaaatgtc aaagcattat aaattaaatt ctcaaatgct
    15901 cttgtaagca tctctttatt tatctttgca tatatttttt tctccaatat gtaagtttca
    15961 aagtttacac cacatgcttg tgtatctggc aagttatttt catatatctc tttaataaaa
    16021 tcatttagac atattttttc actaatacca atgcttttat cttgtgatgt aaattcttgc
    16081 aaactacgta ctctattttg tacttcattt gacttttgat ttatagcata aagataggtt
    16141 cttgtctctt catctacatg aatattacca acctttatca agtctataaa tgattgtata
    16201 gctgaaacag gtgcctttaa atcatgtgcc acatctgcca agaatttttt acgttcattt
    16261 aacaatgttg taagttcatt agttctcact tcaacttcat gttcaagatt ccttgtaagt
    16321 ttttcattct cttttattat tattctattc ctgtttatca tcataagaga aaataaaata
    16381 acaattataa atccacaata ttctgtttgc caccctgtaa atataggctc aaatctatta
    16441 ctagtaactg catcaaataa tatgctaatt ccaaaaacag tgtttactga aactaaaata
    16501 taagaactta tatgctcaga gcatgtcttt ttatatattg aaaccagcga agacattaat
    16561 atatatataa atacaatata tttatatatg tctattaatt tcccatatat gttgacaaag
    16621 tacatatcat atggaagaag aataattggt atcactatag atatgatact cattgtaatt
    16681 cctaatggta aaattaaaaa tctatacact ttatcttctt ctattttgga tatacttcca
    16741 tttatacaaa taatacataa aactaccata aaataggacg aatcttcaac cgcatatgta
    16801 aagcttacca aaggcagtcc taacaaatgt acaaagggat aggatacatg gatggcaaag
    16861 aaaacacaca tacatccaaa atataagtaa agtttacttt tgtttgaaag aaaccataca
    16921 gacaacgaaa atatagctat tgcaaaagtt gtgaagcaaa ttatactata aaaaagtaat
    16981 cgataaaaca tcatattact tatgtccttt gtttctccta atgcaggcgg ataatacaaa
    17041 ccactataat aatgtgaaaa attcgataca ttgattataa tttctgtatc tttctttgca
    17101 ataaaactta ctactgaatt tttaattttg ggtctatatt tttctgttcc tacatctcct
    17161 aatttagaaa ctatttctcc atttatccat acttcacttg cggaaaaaat ctcaggaagc
    17221 tctaaactta gtagcttatc ctcaccttca ttttttattc tcatacgata agtagcattc
    17281 ccatatggtg actgattccc ttttctcata gaaaaatctg gatattgacc tataaaaaca
    17341 tattctggtt ttatatcttg ctctgtaaaa tcattcggtt taaatatttt atgattataa
    17401 aactcccagt catctatcag aaagacaagt ttacctttag tgaaagtatc attatctata
    17461 gttaaaattc cattacttgc tctaatagac ttatatgtat atttattatc atatttatac
    17521 agaagattta atcctgctat tgccaacaga gagactatca atacaatgag agttcctttt
    17581 agtatattat actttttcat tgcatcaacc acatctcttt catagtagta ttaaataaaa
    17641 caagaactat ttattaatta cagtatctca cttatggtac aaacttatat ccttttcccc
    17701 atacggtttg aatgtattga tgctccctgc ttaaagaatt aatttttttt ctcatctgtg
    17761 caatatgaac ctgcactgta tgttgatctc caatatcagg cattttccaa acttggttat
    17821 atatctctat cttcgaaaat acttgttctt tgttttcagc tagaaacaat aatatttcaa
    17881 attccatagt agttaaatct atactttctc catcaaaagt tacttttctt gaatttacat
    17941 ttattactaa cgctgaaaac tttaacacat taggaactga tgtaatattt ttgtactgcc
    18001 tcatacgagc atatattcta agttcaagtt cttttaaact ataaggtttt gttatataat
    18061 catccccccc agctaaaaat ccccgaactt tttcttcttc atgctcataa ttagataaaa
    18121 atattatagg tatgttaacc ttttctttta tatcattgca cattttatat ccatctatat
    18181 ctggcaatga aatatccaaa attatacagt ctaaaactat acgctctact atatccatag
    18241 catctttagc agtctctgat gtatatacat taaaacctat attagaaaaa taaatttgat
    18301 ttatatttaa aatatcaaca tcatcatcta caaccagtaa attatacatt acaccactcc
    18361 attcaaaggt attttaatca taatctcaag ttttataatt attacaaatt ataatgtttt
    18421 attttcatat ataactagta ttattatttt ataatatctt tacaaactag actataatac
    18481 ttaagtattt ttgaacaatt cttgaatatt gtattgaaca ttaagattta ttattaaata
    18541 ctaattaaag tctccgattg ttggagaatg gagcttaaag tcattttcac ttataaattt
    18601 attatttttc tagaaacttg tctattggct agtttgagta ttgatttata tgatttatat
    18661 ggttataata taaaataaat tgtattattt tggtaaatat attgttacaa aaggtttata
    18721 ttttgcatct caatgtttat aaaaacttta ttttcttatt ataatagtat tatacttttt
    18781 aaaaaatttc ccttttccaa caaatggcta ctttaaaagc caaaatttca cctatcaata
    18841 aaaatcttaa cgactaatac tcttattttc aaattttgca acatttttgt taattttaaa
    18901 gcaaaaaaag actatgcaat ataaaaatta tattcatagc cggttgcacc actaactcca
    18961 tatatctcca gatgcccaat aataagatat acttctcagt ttctaaaata tttttattaa
    19021 aaatctttta tatattttca taaatttctt ttttattcta acattgtaat attattttgt
    19081 caatatttcc atatttttag atataagata ctaaaatacc tattttatct aaagatattt
    19141 atatttctac attcaataat agacaatacc ctgcaagtta aagcatcatt tttatgagct
    19201 tatctcacag ggtattatat gtttacttta tattataatt catgttattg attaacttta
    19261 ataaatcctt tttctgaaac aagtgcttgt ccttcatcac ttaatatata atctattaaa
    19321 gctttccctt tttctgacaa accatcttct ttattaacta atataaatgg tcttgatatt
    19381 ggatatttat tagccattac atttgcatca gttgcttcaa catcatttat ttttaaacta
    19441 tttactttct catcaacata tccaaatgat atatatccta tagcattttc atttccttca
    19501 actgtagttt ttatatttcc tgaaccatca cttatttgtg catctttagt tagttcttct
    19561 gaatcaaacc ctacaatttc ttggaatcca tctctagttc ctgaaccttc ttctcttgat
    19621 actattacta ttggtgcatc atttcctcct acttctttcc agttagtaat ctttcctgta
    19681 tatatacctt taacttggtc cattgtcagg ttttttactg agtttgcagt attagttatc
    19741 aatgctatac catctattgc tattttagtt tcctttaatc cagcactttt ttcttcatct
    19801 tttaaatctc ttgaactcat tcctatttca gatgttcctt ctatggcatt ttttatacct
    19861 ccggaagaac caatctgttg tacttctaca ctaacacctt gatttttttc ttgaaacttt
    19921 tctccgatag tttccataag aggacctatt gaagttgaac ctgaaacaga aactttgctt
    19981 gaatctcctg aatcacctga acctcctgag ttagaactac atcctactgc taaagctcca
    20041 actaacattg tactagctaa aagaacgcct attttctttt taaacatttt acttaccccc
    20101 gcttatattt taattttttg ttgtcatttt tctatatttt aataatacag attctttgtt
    20161 aaatgaatat tagagcaggg ttaagtaaat gtaaatattg ttaaatacat gtaaaaactt
    20221 aattttatat gatttattct taattagatt aactttttaa agttacttta ggcagatata
    20281 aacacaatac aactcatatt atcagaagtc ttcattgaag ctatcaacct atcacatcct
    20341 ataattcttt ttctagttta ttaactaatt cagcaaatac atttaaggct tcctctatag
    20401 attctttttt agtcatatct acaccagcag attttaattg ttctagtggg tattctgacc
    20461 ctccactttt taagaagttt atatatctat ctactgcagt acttccttct gaaagtatct
    20521 gttgacttaa agcacttgca gcagaaaacc ctgtggcata cttatataca tagaagtttg
    20581 aataaaaatg aggtattctc gcccattcta ttcctatttg tttatctact attgcagatt
    20641 ttccgtaata cttttcattt aatttgtagt atatattagt aaattcatca gcagtaagtg
    20701 gttctccact ctctaccctt tgatgtacaa ttttttcaaa ttcagcaaac atagtttgtc
    20761 tatgaactgt agttctaaat tgttctaaat aataatttaa taagtatata gtttcatcct
    20821 tagacttaga attttctaat aaataattta taagaagtaa ttcatttaat gtagatgcaa
    20881 cttctgcaac gaatatctta tagtccgaat ataaaaatgg ttgacttgtt cttgaataat
    20941 gactgtgaac agagtgccct aattcatgaa ttaaagtgaa tagagaatta aggtcatttt
    21001 tatagctcat taatatataa ggatgtgaat cataacttcc ccaagaataa gctcctcctt
    21061 ttttaccttc attatcatat acatcaatcc acccttcatc aaaagcactt tttatgacct
    21121 tcaagtattt ttctcctaaa ggtttaagtg cctctaatat gatatcctgt gctttatcat
    21181 atggtatatc catgtcaaat ttatctgtca aaggaacata caaatcatac atgtgtattt
    21241 cattcaatcc taaaaatttc tttttaagct caacatattt atttagatta ggaatacttt
    21301 tatctatagc tgatataaga ttattgtaaa catctaaact tacatcatct gaaaataaag
    21361 acgcttctat tgctgaatta tactttctag tttttgcgta aaatatctct gattttattc
    21421 caccataaag cgttgaggca aatgtatttt tatatttctc atatgttgaa aattcacctt
    21481 caaaagcgtc ttttctaact ctactattct tactttttat aaataaagag tagtttgaat
    21541 gagaaagctt aaccttatta ccctcctcat cttctatctc aggaaattcc atatctgcat
    21601 atgctaacat atcgtataca ttttctggaa ttgtcgataa ttctgatgta gctgcaagta
    21661 tttcttcctc tcgttcactc aaagtatgtg gtttatctct caatatatcc tttatatatt
    21721 ttgtataaaa ggatagcttc tcatctttta agtattcact taacttattc tcttctatcg
    21781 ccagtatttc tggaactata taagaagtag ccacacctaa atcagtagat aacatttctg
    21841 tcttagtggc tcttccttga ttatcattaa ttctagtatc ttcatgttgt ttcatatgag
    21901 tatatacata taaattttga acaatcctag atgcattttc agagatattt aatactttat
    21961 ataagttctc cttactctca gaaagcttcc ctttatattc ttttacttct tggataagat
    22021 tttttacttt acttatgtct ttttcaattt cctctttact ggagtacatc ttatctatct
    22081 tccatttgta cttttcttct attgtacttc tatcagttcc catatcatac acctctattt
    22141 taatttgttt tatattttat tcactttaca aaatatattg tatatatttt agtccaaata
    22201 atctacattt attactcctt ctatcatatt tatttctttc ataacgtcgt gtaagtgtat
    22261 tctcttagca taagatacaa aaaaattaac cttccagtat tcttcattac gacattctat
    22321 ttccatattt cttacgataa gttgattttt ttcaaattta tcatataaaa aaggtataga
    22381 gtccaaattg cttaatttta atatcaatac attcttttct tggctaaata aaaacgtatt
    22441 gattatccta agcacaaata atgctatcat tacaaataat ccacctagaa ttgccaattc
    22501 ataataacca tatccaactg ctataccaat acatgcagtt gcccacatcc cagcagctgt
    22561 agtaagccct tttactccac ctttattttt taaaattgct cctgcaccta aaaaaccaat
    22621 tccagataat acttgagcag gcattcttgc tgggtcagca tttgtataca aattataatc
    22681 aaagaataat tgaatcgaag ttattgaaac aatacaagaa cctattgcaa ttagtatatg
    22741 tgtcctaaat cctgcaaact gtcttatttt ttctctctct aagcctatta ttcctcctat
    22801 tattagtgct aatagtaacc ttattgctat ctccttactt cctatatcaa tcataatata
    22861 aaaccctcct gacttaatta caacttatgt cctacagata atttacacat cagaaatact
    22921 attttatatc ttaatattat tattaatata tgtaaaatat agccatctgt taatttttga
    22981 tacaaaaaaa cccacaactg tgggctattt gcttaggtac atgtatttat ttattaatat
    23041 atctattttc ttacttgatt ctactaactc ttcttgaaat atttctggat attctatgta
    23101 tgtatttatc tcttccctaa ctgcttcaat ctcatttttt aacgcttcta attcattgtt
    23161 cgccataata atcaccacta ttacaaattt gtatattttt atatacattt tatcaaacaa
    23221 tattatattt tgtcaatatt atgtcaataa aacttactat aaaaatgctt taatttaggt
    23281 attgtatacc tggatactaa gcctacaaac ataccagtta ctaatgatgc cattaacata
    23341 aatggtaagt accctatagc aagtatattt tctatgatta aacttgcaac tattaattga
    23401 cctatattat gccctattgc acctataatg cttatcccta ttagtgataa atcttttatt
    23461 ttactcgcta aatacataaa taaaagactc agataagctc ctcctatact aaatagtaga
    23521 tatgataaag ggcctgcaaa tattgatgac aaaattaccc tgatagtaac aattataaca
    23581 gtatctttga cacctaacaa cattaatgaa attaaagtta taatattgct caaacccaat
    23641 tttgcccctg gaaatataac tataagagga tttggtatat atgtctctag tacatataaa
    23701 gctaaactat accctatcat aagacctaaa aatgtcattt ttctagtttt cattaagtta
    23761 actcctccaa attttatcta tcatcttaca ttataataca aaatatataa aaacacctaa
    23821 tttagactaa aatattttta atctatttag aataaatatt agttatttca atgtgcaatt
    23881 acctctataa atcttgactt tttgtatcat acttgttaca attttaatga taatgaattt
    23941 cattaggagg tgttaataat gactgtttac gatttaaaaa taggagaaaa aggatttatt
    24001 aatagtatag atggagatat taaattatct aaaagattgc ttgcacttgg atgtataaat
    24061 agtactgaaa tagaagttaa aaaggttgcc ccacttggag acccaattat aattagattt
    24121 agaggctttg accttgcgat tagaaaatct gatgctaaaa atatttcttt acaataatag
    24181 aggaggagct acccgtgatt aatgtagcat tattaggaaa cccaaatgtc ggtaaaacta
    24241 ctgtttttaa tctacttaca ggttcaaatc aatatgtagg taactggcct ggtgttacta
    24301 tagaaaaaaa agaaggattt cttggaaaag agataaaggt tgtcgattta cctggtatat
    24361 atgcaatgga tactttctca aatgaagaaa aggtttctaa atcatattta gaaaatgaag
    24421 atgttgatgt aatagtaaat gtagttgatg cttctaattt atcaagaaac ctttacttaa
    24481 caactcaact tatgcaattt aacaagccta tagttatatt gcttaatatg ttagacattg
    24541 cagaatctaa aggagttaat atagatgcca aaaaattaag tgaagaactt ggagtaattg
    24601 ttgttccaat tatagcaaaa aagaaaaatg gtgttgatag gatagaagaa gagattaagg
    24661 gagctacgca aaaagctttt atatatcaga aaaactttgg aagtgaaaca gaaacatata
    24721 aaaaattaga atccatttta tcgagatgta caaaaactac atatacacaa aagaaatcta
    24781 tcagtgataa gatagacaat atagtcttga atcctatatt agcatatccg atatttattg
    24841 gtgtattgtt cctcttattt aaatttacct ttgactgggt cggaggacca ctacaagagg
    24901 gatttgctag tcttatagag gcttatattt ctgcacctgt tagtgatatg ttatctaact
    24961 ctagcccatg gtttaaatca cttatagttg atggaataat tggaggtgtt ggtggtactt
    25021 taccattctt ccctttaata tttacattat tctttggtat atcgttgttt gaagatagtg
    25081 gatacatgtc aagaacagca tttttaatgg ataaagttat gagaaaggtt ggtctatctg
    25141 gtaaagcttt tatacctata gttatgggta tgggatgttc ttctccagct ataatggcaa
    25201 ctagaacatt ggaaagcgaa aaagatagaa gagttacagc tcttattgca ccacttatga
    25261 cttgtggagc aaagctacct atatatgcac tatttgtagc tatattcttt ccacataatg
    25321 ctgctttagt aactacgtca ctgtatttgg tgggtatagt tatggcaatt gtagttgcat
    25381 tattcttaaa taaaacagcc tttaaaacag aggctgaacc atttatatta gaattaccag
    25441 aatatagaat ccctactata gatgcactta ttaaaaatac ttggaataaa tcaaaaggtt
    25501 tcttgataag agttgttact gtcatgtttg caatgtcagt cgtaatttgg ggactttctt
    25561 cattcaatgt ttttggatat acagaagata taaatgtaag cttcttggct actctaggtc
    25621 atattatatc tcctatattc aaacctcttg gttttgatga ttggagaact tcagttgcta
    25681 ttttaagtgg tcttggagca aaagaaatcg tagtaaactc tttaaatata ctatatggta
    25741 atctaactgt tgtcttacca acaatattta atggagttac tgcatataca ttcttgatat
    25801 ttactgcttt atatactcca tgtatagctg cacttgcaac tatgaaaaaa gaatatggca
    25861 ataaaatgat gtttacatca tttgcatatc aatttatact agcatggata atggctttta
    25921 tagttaaaag cataggtgga gtactattta tgggtaattc aataatggaa gctttgatag
    25981 gtggaataat tatagtcctt gcactcttta ttctgtttaa taaattcaag tctacaaaaa
    26041 acggaaatgt ctgtagtgga tgttctattt gctctggatg tccaagtgct aattcttgct
    26101 caagtaagga agacagcaat aaaaaaagta atacacacta atatataaat attttagttt
    26161 aaaataagat aaaagtgggt gtctcaaaat taacttttta gttcatgagg cactcttttt
    26221 tatataaaat caaatatatt ttcatcattt caacagtcaa aaagaggctt atctctattt
    26281 tgagacaacc tctttttttg ttacttattc agtttcttgc tacctaactt catgtggaac
    26341 ctagtatctt ttggaacact tgggtctacg cctcttaatg taggtatttc cccacatatt
    26401 agttgtatag gtactataaa attaaatgtt tgatagtaag gattatctgt gatatcaatc
    26461 tttaaatttt tatcattttc tgtaacatct ctacctatag caaatacatt ttccgtccaa
    26521 ccagaaagta catcaatcat cttagttact ctaggttctt ttcctgtatc tagtataaat
    26581 atagtagaat cactatttat agcattatat ataccgtgta taaactcttc aaactcatac
    26641 ccagttacag gtattctcat tgtttccaat aattttaatg ctgcttctaa tgtatctcca
    26701 tatatatcag agtgacctat tattcttatt tctttagaat taactaactt ttctttgttt
    26761 ctttcaatcc attgtttaga gagcttatac actgcttcaa atctattaat tgcatctaat
    26821 atcttattta tttcctcatt gtacttttca ctagatattg tacctttttc tctagctatt
    26881 tgaagaccta ataacattaa gtttaacttt gtacaataat atccttttgt tttagctcca
    26941 gatttttctt caccacaatt tactgttaat atataatcag aaatctcatc tattaaagca
    27001 ttcttacaac ctgccataga tgctatttta caacccttat cttcagctaa cttcatagcg
    27061 ttgtatgtag aataactact cccaccttga gatacaccta ctactagggt attttcatta
    27121 tcaaacttga atgtatcttc agttatcata aatggataca ttttcacaac atctatatct
    27181 aataaatttt gtaaatatgg ttgcacttgc actcctgagt gatagctagt acctgaacct
    27241 gttattataa tcttttttag atttgctctc ttaacttcat taaataagct atctgcatta
    27301 gatataattt ctctcattct aactggtgtt tctaacatat aatcttgtat tgtcatttta
    27361 tacaccttcc ttatctatat attcaataaa ttataattaa tttctataac aaacctatag
    27421 catgtaaagc gattcctgtt actatagtgc ctatagttaa ttttgttaca cttacctttc
    27481 tcttcaacat gtaatatatt aaaaatgcaa aggttaaagg taacatatta ggtattattt
    27541 tatctagtat atcttgtaaa attacttctg caccatttaa gttaaatttc aaaggagtag
    27601 ttatactcaa catagttgca accattcctc ctacaacgaa taaaccgaca gttgtagcca
    27661 tagacattat cttctccata agtccctctc tttgtattct ttcaagagaa tttaccccta
    27721 ctttataacc atacttaagt ccaaatattc taagggcaaa tgctggtata ttgtataata
    27781 ataagaataa tagtggtcca aagatattgc cttcttttgc aagtgatact cctactcctg
    27841 ctgctataat cctaaatgtt ccccagaata atgaatcacc aattcctgca agaggtccca
    27901 taagagctgt tttaacagca ctaattgaac tttcatcaaa ttctggatta ttagcatttt
    27961 cttcttccat agctgctgtt atacctaata tagcaggaac taccactgtc gttgtattaa
    28021 aaatctctaa atgcctttct attgcttttg cttgattttc tttcttatta tatagcttct
    28081 ttattgcagg taacattgaa taacaataac ctaagttttg cattctttcg taattaaatg
    28141 caccttgaag agcaaatgaa cgccaaaaca tacttcttaa ttcttttttt gttaatttat
    28201 tcaaagtcaa tctctccttc cgttaaagta ttcattgcta attgaggatt ttgattattt
    28261 tctttgttat tgatataaaa gtttagtaca aatgctacac atactccaaa taaagctata
    28321 gcagttatat ctaatccacc ataagatgct aacaaaaacc ctataaagaa aaatacagac
    28381 atcttttttg aaatcaacat atccataagc aacgcaaaac ctactgctgg caacaatgtt
    28441 cctgcaacac ccaatccgtc taatataact ttaggtattg catttatcaa tgttgtaact
    28501 ttatcagcac caatcataat tgctaaaaag gttggaactg atgtacttaa aaagaataat
    28561 attactggca accacatcat tatggtaact ttcttaaaat cagcctgttt agcatagaaa
    28621 actgcctttt gagaaaagta cgaatttatt attctaacta atactccaag agattgtgct
    28681 aatactgcta ctggcactgc cactgctaaa gcaacttctg caccattacc tgataatata
    28741 gcaaaagctg tacccaatac acctcctgtt actacgtctg gaggagtagc tgcacctatc
    28801 cctgcaattc ccatccaaat taattcaagc tgtgcaccaa tcataattcc tgattttatg
    28861 tctcctaaaa ctaatccaac taacatacct gtaactattg gcctatcaaa catagattga
    28921 ccaaatatac gcccatctag tataccaact cctgctataa tagctaacat agtagcttgt
    28981 actaacatat tgaacctcca ttttaactta gtttaattta cattaaatta cattacattt
    29041 ttgattttat ttcgattttc taataactca tttacataaa caattccatt ttttgcttcc
    29101 tctaagcctg atttaactat tagttttgta tcctcctcaa ctaataagaa tatagataaa
    29161 agcattggta aatttacgcc tgtcaacaat tcaaatttga acccttgcaa aagtaagttt
    29221 gtacatatat ttgccacact tccaccaaac acatctgtta aaacaacaaa ttcttcacta
    29281 tctttattat ttgataatat acttctaatt tctttctcag catcatctgc tgatttatct
    29341 tttgtcatac aaaaatactc aatattagct aatgaaccaa ttattaattt acttgtgtca
    29401 acaagttctc tagacaattc tccatgtgtt gcaattagaa attttttcat tctctttctc
    29461 ctaaattatt ttattaaatc tgatagttgt ttctttttgt ctctaggtaa cattctaagt
    29521 tctatatcaa ttcctaaatc acctatttct ttaaagttat ttatatcatc atcatcaaca
    29581 gcaactaaat ctgtaagttt cttttttcct tcttcatatc ttaaccctcc tacatttatt
    29641 tcttttaata ctcctcctga atttttagct acaactaaag catctctggt attttttact
    29701 aacactaata cattgctttt ctttgaagaa gcaaattttt ctactatttc tccagattcc
    29761 tctacccccc ttatatataa agttacattg ctaggtttcg ctaaattcag tgacatagct
    29821 ttcatttggt cattttttgc ttcatcattt gcaactagta ttacatttat acctagtgat
    29881 gttgcccatc catatgcaac ctgaccatgt atcaatctgt catcaattct catcaaagat
    29941 accattttcc ccaaatcctc catcatacat taaaattttt attgtttata ctctcattta
    30001 tctataaaag caaaaagtat gccaaatact ggcatactat tttaaatttc atttacttat
    30061 tttgtaatct tgtattttac tgtggtagac catagataat attataaata tatcctatct
    30121 ctgaaattgg tatttgcaca ctataactct tttctagcac actaaatgca ttttttatta
    30181 gttttacttc actatgatgg tttttgatta acaaatccat atcagtatac tcttcaattt
    30241 cagtttgtct aactaatctt tcaatcatac aacttaaatg aacatataaa gaaatctttt
    30301 tatcatttgg aagttttttc ttttgaatat tttctatagc attgattcct tcttctattc
    30361 tctctattat cttttttgaa tctaaaatag ttagactgtc tattactcta attaatgaaa
    30421 aattctttac aatagaatca tttatgttct ttattttttc attttctact actctactaa
    30481 atattctaaa aacatcatct tctccttttc ctgatattat atcctctaac aatataaaat
    30541 ccaccccatt cacatttgga ttagcagttc ctactatagc catcacatca tacatttcaa
    30601 aaactggttc tttatctccc atagaaacaa gactatcata atcataaggt attattttga
    30661 catcaactat cccaattaaa ctttcggaaa gtagatgttg gatttttata gcagtaccta
    30721 tccctgtaaa acaagatgat attatagcat attctttatt tttttgagga taatacaatt
    30781 gtatattagg tttagtttct tcatccataa tttctgcaat ctgttcaatg tttttacctt
    30841 gcattaataa atttcctact tctagtgcag aaatagtgga tatttggtct ataaataaca
    30901 ttggcccatt tatcttattt tttaaatctt catagataag attaagagag cccatatcaa
    30961 ataacacaat taaaccattc tttatcctat ctccttctag gtatctagac atttgttttg
    31021 atacatcagc aatggtagca tctataggca tatcaatagc tgtaaatata tttttaccca
    31081 acatccgatt acatacattc gccatactac tggcagtagc atatccatgt gctataatag
    31141 tcgcattaac ttgattaaat ttcattctta taattgttga tttaataaat ataatcatta
    31201 tgattgtatc tacattatta agatacatat ccaatctatt cgttattaat gttgatattt
    31261 tacttattat agagtattct ttgtaactct ctttttttaa atatattagt aatttacttt
    31321 ctaaagcttt atactcatca gataattgat aagattcgtc cttcaaataa agataagacg
    31381 aaaatgccaa tacattattc ccatcgtatt ttatattgtt attaacttct aaatttctaa
    31441 atatatcctg cattatcgat attataagct ccataagtac gttattattt tcatcattat
    31501 ttgaaaagac taaattgtcc attagtctaa tcattagatt agaactcttt ttactaaata
    31561 tttcttttgt tatttcattt ttttctagtc tatcaaagta gtctaaacat tttatgaaac
    31621 tttgatagat tatatttctt ttatttcttc catttgacat ttcaatacta aattgtttag
    31681 gagaaaagat aatgcttttg cttttaccac tttggaattg aaatatatct acattttcta
    31741 tataaagatt tctagggagg tcttgaagag taacaaaaat atttccattt cttctttgat
    31801 tttttgcata agctcgtcca catgcatatt ttattacatt tttacattca cctatattcc
    31861 ccttgaattt tatctttaaa atcgtattta atgtaatttg agttatctct ataggcaagc
    31921 caagtacctt actttcttca taaataaaat gttctacaat ctttcttttt tcaatagtcc
    31981 ctctttcttc tatattggga acataaatat ttatgggtat tcttcttaaa aaagtttgca
    32041 aaaatttgga tagactttct gttgttgcaa atattaatct taccctagct tttctaatta
    32101 ctccattctc tccaatccta gaaaacgtac ctgtatccat aaatgtaaat aacttttcct
    32161 gaccttcact atttaatcta tgtacttcat ctaaaaataa aacaccacca tctgattgtt
    32221 ctattagacc tgctctagat tcatttgctc cagtatatgc tccttttaca tgcccaaata
    32281 atagactaga caataactcc aaattattat aatactgcgc acagtttaac acattgtatg
    32341 gagaattttt ttctaataca ttactgctta cactatattt gtatattatc ttggctaaaa
    32401 aagtttttcc tactccactt tcacctgtca gcattattgt aagtccgcca tttggataaa
    32461 gtacagcggt ttttatttgt tcgattactt cttttaagct attatcatat cctatgatac
    32521 tattaaacac agtctcttgc tttaataatt tacactcatt tttaagttca tctaaatttt
    32581 cataaatact tttttttgta ggtcctacta catcatttat tttattttta ggtaaaaaaa
    32641 taacaggtct actgtttatc tttataagat tatcagattt tactaaatca tttaataatc
    32701 tacttgattg agttctactt aaattaaact tttttactat atcttctgtt attatgccac
    32761 tttctttttt aagttcatca aagttgttaa ataatacaca ttcatttaca aaacttaaaa
    32821 tttcatccct catgttcaac ctccaataca aatatcaata atactataat attagtatat
    32881 catttatata tttatgaaaa agataattat attcctatct tgattctctt ttgtaaggtt
    32941 gtaaaatcaa actttttata agtataaaat ctagattttt taacataatc tcaaggttct
    33001 tatctttcca tatatctgac atagaattta tataagaata tatttattgt att
//
LOCUS       DAGQWR010000027        32948 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.027, whole genome shotgun sequence.
ACCESSION   DAGQWR010000027 DAGQWR010000000
VERSION     DAGQWR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.027"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..3344)
                     /locus_tag="MIW95_001110"
     CDS             complement(<1..3344)
                     /locus_tag="MIW95_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBY3150455.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDIINQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGIGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADVEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWN
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVINLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQTVVV"
     gene            4135..4455
                     /locus_tag="MIW95_001111"
     CDS             4135..4455
                     /locus_tag="MIW95_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBY3150456.1"
                     /translation="MEKIMNFLKENSTFYFATVEDNKPRVRSFGFIMDYEGKLYFGIV
                     KYKTSYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAK
                     NSLV"
     gene            complement(4404..5150)
                     /locus_tag="MIW95_001112"
     CDS             complement(4404..5150)
                     /locus_tag="MIW95_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBY3150457.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNSIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5168..8605)
                     /locus_tag="MIW95_001113"
     CDS             complement(5168..8605)
                     /locus_tag="MIW95_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBY3150458.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPIFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEISKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENILGGIIITEFNDDFTIKYTNSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIIDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSEMVKTISNSIIHKDNKEVFLNTFLCKNLLSAYNKNITEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIKLKLKAERDTLTGLYN
                     KGTTENLINDFLNSDEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKHLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENTKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNNCIYQNELENNISDNNIKLANK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSER
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            complement(8638..10119)
                     /locus_tag="MIW95_001114"
     CDS             complement(8638..10119)
                     /locus_tag="MIW95_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY3150459.1"
                     /translation="MKKNRLIIKNISIAFIVLFFFSVFTFFYIGNISRVLEYETNDIM
                     TVTITGWIILSFLFLCIIIYILYSKAKSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIIANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFLKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            complement(10387..11226)
                     /locus_tag="MIW95_001115"
     CDS             complement(10387..11226)
                     /locus_tag="MIW95_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBY3150460.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            complement(11247..11780)
                     /locus_tag="MIW95_001116"
     CDS             complement(11247..11780)
                     /locus_tag="MIW95_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBY3150461.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            complement(11792..12601)
                     /gene="aroE"
                     /locus_tag="MIW95_001117"
     CDS             complement(11792..12601)
                     /gene="aroE"
                     /locus_tag="MIW95_001117"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBY3150462.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRIFEILNIESAYKNFEIPKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            complement(12648..13844)
                     /gene="pheA"
                     /locus_tag="MIW95_001118"
     CDS             complement(12648..13844)
                     /gene="pheA"
                     /locus_tag="MIW95_001118"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBY3150463.1"
                     /translation="MEDLTRYRNEIDNIDKEIIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAIDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKTISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYELEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAENNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            complement(13847..14926)
                     /gene="aroC"
                     /locus_tag="MIW95_001119"
     CDS             complement(13847..14926)
                     /gene="aroC"
                     /locus_tag="MIW95_001119"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBY3150464.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDKIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQRDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            complement(14904..16217)
                     /gene="aroA"
                     /locus_tag="MIW95_001120"
     CDS             complement(14904..16217)
                     /gene="aroA"
                     /locus_tag="MIW95_001120"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBY3150465.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNSTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            complement(16217..17281)
                     /locus_tag="MIW95_001121"
     CDS             complement(16217..17281)
                     /locus_tag="MIW95_001121"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBY3150466.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            complement(17303..18316)
                     /gene="aroF"
                     /locus_tag="MIW95_001122"
     CDS             complement(17303..18316)
                     /gene="aroF"
                     /locus_tag="MIW95_001122"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBY3150467.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKIVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            complement(18446..18601)
                     /locus_tag="MIW95_001123"
     CDS             complement(18446..18601)
                     /locus_tag="MIW95_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150468.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            complement(18778..19734)
                     /locus_tag="MIW95_001124"
     CDS             complement(18778..19734)
                     /locus_tag="MIW95_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBY3150469.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFIGIIIFKIKKLL"
     gene            complement(20073..20771)
                     /locus_tag="MIW95_001125"
     CDS             complement(20073..20771)
                     /locus_tag="MIW95_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3150470.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            complement(20805..23507)
                     /locus_tag="MIW95_001126"
     CDS             complement(20805..23507)
                     /locus_tag="MIW95_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBY3150471.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEVITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSSKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            complement(23685..25502)
                     /gene="ftsH"
                     /locus_tag="MIW95_001127"
     CDS             complement(23685..25502)
                     /gene="ftsH"
                     /locus_tag="MIW95_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBY3150472.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            complement(25533..25976)
                     /locus_tag="MIW95_001128"
     CDS             complement(25533..25976)
                     /locus_tag="MIW95_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY3150473.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            complement(26467..27357)
                     /gene="metA"
                     /locus_tag="MIW95_001129"
     CDS             complement(26467..27357)
                     /gene="metA"
                     /locus_tag="MIW95_001129"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBY3150474.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(27387..28652)
                     /locus_tag="MIW95_001130"
     CDS             complement(27387..28652)
                     /locus_tag="MIW95_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBY3150475.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(28762..28864)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(28918..31575)
                     /locus_tag="MIW95_001131"
     CDS             complement(28918..31575)
                     /locus_tag="MIW95_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBY3150476.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNFSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKRKK"
     gene            complement(31703..32446)
                     /gene="yaaA"
                     /locus_tag="MIW95_001132"
     CDS             complement(31703..32446)
                     /gene="yaaA"
                     /locus_tag="MIW95_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBY3150477.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIIN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            complement(32456..32920)
                     /gene="bcp"
                     /locus_tag="MIW95_001133"
     CDS             complement(32456..32920)
                     /gene="bcp"
                     /locus_tag="MIW95_001133"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBY3150478.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
BASE COUNT        10361 a         5664 c         3664 g        13259 t
ORIGIN      
        1 acaacaacag tctgattgct tcgttttact acagaaatat ctgctgatga acttacctct
       61 gcttctgatg ttgaagtatt gttatttaga tttgggtcag gtgtagttga acttactgtt
      121 gctgtattta taattgtacc tattgctgtt tgacttactg ttccccttat tatgatattt
      181 cttatctctc cagcatctaa agtacctata ttaaggcttc cattccatgg ttggaaagtc
      241 actccattat ctattgagta ctctggattt aaaatagaag ctggtatatt atctgttaaa
      301 gttacgttct ctgcaaaaga tggtcctgca tttgcaattt caattccata aataaatctt
      361 ccccctggta tagcaactgg ttcgttgcct tttatcacag aaatatctgc taatggactt
      421 atatctgttt catctgttga agtattatta tctggatttg ggtcaggtgt aggtgaactt
      481 attgttgctg tatttataat tgtacctact gctgtctggc tcactgttcc tcttattata
      541 atacttctta tttctccagc atccaaagtg cctatactaa gatttccatt ccatggttgg
      601 aaagttactc ctccatccaa tgaatactct ggatttaaaa taacatctgg tatatcatct
      661 gtcaaaacga tactttgtgc agcagatggt cctaaattta taactgttat agtataacta
      721 aaagtttctc ctggaactgc tgtatccata ttgctagttt ttattacttc tatatctgct
      781 gatggaacta taggagttgt cactgttgat gaatccgtaa ctggttctgg tacatctgca
      841 cttacttctg ctgtattagt aatatctcct gtagcagttg aacttactaa tccctttatg
      901 ataattctta ttaattgccc tggtgcaact gtacctatat taagacttcc attccatggt
      961 tggaaagttg ctcctccatc caatgaatat tctggattta aaataacatc tggtatatca
     1021 tctgttaaaa ctacattcgt agcattagat ggtccttcat taaaaatttc aattgtatat
     1081 acaaattctt ctcctggaac agctacatcc tcgtcagcta cttttataat agaaatacct
     1141 gcttgtgggt taactggtgt aataatggta gatatattgt tgcttaaatc tgggtcaggt
     1201 gtagttgaag aaactgttgc tgtatttact atatattctg aagaatttaa actaactgtt
     1261 ccttgaatta aaactgttaa tgaaattcca gagtttaaag ttcctatatt aagacttcca
     1321 ttccatggtt gaaaacttgc tcctccatcc aatgaatatt ctggatttat aatagaggct
     1381 ggaatatcat catttaatat tacattttca gcatttgctg gtcctaagtt ttctacttga
     1441 atagtatatc tcaataaagt acctggaaca gctgaattag gttctgctgt tttcattact
     1501 aaaatatctg ctaataaatt cacttctgtc tctattgtag atgtattatt attaaggttt
     1561 gggtctgctg tagttgaaga tactacagct gtatttgtta taattcctac tgttgatgga
     1621 tttacaatac ccctaattat aataactctt gtctctccta cttctaatgt ccctatattt
     1681 aaacttccag tccatggttg aaaagttact cctccatcta atgaatactc tggatttata
     1741 atagaagctg gtacatcatc tgttaaaacc acattctcag atgggaatgg tccattattt
     1801 actactttta tagtgtatat taattcctct cctggattca ctatagtagg ttcagataat
     1861 tttgtaactt caacatctgc tgagattact tcaacaggaa ctggattaga atctactgaa
     1921 aaatcatttg gtataccgcc ttctacaggt gtataggagt aatctatagt agctatatta
     1981 ttagtaggat ttgggttagg aatactttca accacaacat caaaagttac ttcaacccct
     2041 attcctcctc caatatctgg aagtggaaaa cctgtattag ggtctactcc tggtacagat
     2101 actccatcaa ccgttacagt tccaggtata aactctaaac catttggaat taaatcccta
     2161 aaaaatacat tagttaaagg gctatcacaa gtattatcta atgtaactgt atatgtgatt
     2221 atatcaccta cattagcagt ttctgtgtta cttgttttta ttggagtaaa ggttggaaca
     2281 gctatttctc ttaaagaaac atcatctata gcatagtcat ttccaatggc gctttctcct
     2341 tcactaaaaa attctactgt aagttgatta ttattttgag aattaatcac agtacctact
     2401 tctttccatt ctggtgcgtt tgtattaact ggaatttgag ctcctaatgt tgcttgatat
     2461 agtatattat tattttggtc aaatattctt acacctagct gtggtggtgg atatcctgtt
     2521 accttaaaaa ggtttaaaat ccaagtgcta aataaataat tagtgttagg tgtaacttgt
     2581 actgttgatt taaaaaatac agctccagga ttaaaaccat ttaccaccat cattcttcct
     2641 gtttcatttc ctgtagtatg gtcagcaata cgccaccaag ctcctataac attactcatt
     2701 gcatttgtca taatattttg tactgtatat tctccatcta aaggtgtaaa cttagttggg
     2761 tctggtacaa catacgtaaa atctggcgtc acatttgggt aaggttctat ttcaggtcct
     2821 gtattacttg gtgtacctgg tggaaaaaaa ccgaatgttc cgttatctgc atctgtaata
     2881 aggtttatat tagatacaga cacgcaagaa tccaatatat tctctatagg tgtataaatc
     2941 acaggcccat ttaatatatt ttgatttatt atatctgaac ctgctactgt tattaatagt
     3001 gtgttaggtg ttgtaccatc taagtttgtt gaaccaatag gaggatttgt cacagaacta
     3061 ataggtggca tttctgcaat tggaactgga cctactatcg ctgttgcaaa attccctggt
     3121 gttggtactc ctcctgtagt tccaaaagct tctacaattc tatagttacc atttggaaca
     3181 ttagtaaaaa cataatttcc tgatgcatct gtgagaacca caagtctaac tccagttgct
     3241 atatcttgta atacaactgg tatattggct attcctgagt ccccagaatc aatggtagca
     3301 cttctatttc tatcaaaaac tatttgtcct gatactgttg ccatattatc actcctttgt
     3361 gattacaaaa tttgtgttca taatatatta ttctttgaaa ctcttttatg tgacatttta
     3421 agcctataag tgcgcttttt ttctataaca tctaatatgt atttttttta tttgcaatat
     3481 caattttttt atattttatt gtatatgtta aaaaatatct tatgtcataa gttaaaaact
     3541 atcaaacaat agtaaatggc ataaaaggta ttattttatg ccatttttca ataatcaagt
     3601 attttcaatg tatttataat aacaaaatta caatttgtca gtgaaatgtc agtgattttt
     3661 ttgttatttt ttactctttt taatttgaga agattaagaa tatgctttta tattataaat
     3721 catcaaacaa aatatcgtaa aatttaaact taattacttg agatattcag agatataatt
     3781 ttttgtattt tatttttatc taattgagtt tagagggtct atttttctcg aaaataacaa
     3841 gcttttatat tgctttttaa atgggtttaa tttttttcgg tgatagtttg tacctttcta
     3901 atcaaatcaa gcttaaaatc tacttaaata cttaatctgg cactttaatc taaaatcaat
     3961 aattttagtt tactttattt ttattatgta aactgaaatg tttttctgaa ttttatatat
     4021 aattttgaat aatcaatata atattgtaat tgtttttata tcaatttata ttagtattat
     4081 aaatatttta tttatattca ttatattagt ataagataca aagaggattt aatcatggaa
     4141 aaaataatga attttttaaa agaaaactca acattctatt ttgctacagt tgaagataat
     4201 aaaccaagag taagatcatt cggatttata atggattatg aaggaaaatt atattttgga
     4261 atcgtaaaat ataaaacttc ttataagcaa ttacttgtaa atcccaatgt tgaaatttct
     4321 acaacaagta aagttggaaa atggattaga attaagggaa tagaaaagac tagatatgag
     4381 tttatatcta gtctttttat ttattatcta tcaaaaatga ttgttttcca taatgcaaaa
     4441 aattctcttg tatagtaagt tggtacaaat gaaagttttg atttactagc ataaaaagac
     4501 gtatttttat atccatttct atatgctaat attttacttc tgagaacatg aaaatctgtt
     4561 gttactattt ttacttttaa atcacttagt tttctttgac tatgttcttc tatcttcaat
     4621 tttgaatatt taaaattctc ataagtattc gtggatttat cctcttctat tatcctattt
     4681 ttatctatac tattttttat aagataatct ttcatagctt ttgcctcagt tattttttct
     4741 ccacttcctt taccaccaga tactaccaca taacaattat cattaatttt taggtatttc
     4801 aatgcagtgt ctaacctacc cttcagggtt gtacttggtg tagtcttttt tacagaagct
     4861 cctaaaatta agagataatc actttttgat tctaaactat ttttaggata aaaaataatt
     4921 aaactctcag taactacaaa tactattaaa aaaatgctga ttattatttt taacacccta
     4981 taaaaaacct tcttttcttt aatttttttc ttaaatattt gatagataat tagtacaatt
     5041 cctacaacta agaaaatctc actaaatgaa attcctccaa aaactatttt gagataaaaa
     5101 tagtaaattg taaatattat accaagtatc aaagttatac tatttaacat atcttcatag
     5161 tcctttttta tttatttttg tataaagaag aatctataca ctctataaga cagttgtatt
     5221 ctccatctat gcaattaatc caacttgcta tactgatatt tttaccaaat gtatttgtct
     5281 ctattttgaa ttttgaatgc ttattttctt ctaatccgtt aagagggcaa ttattacatg
     5341 gtctctcaga ttttaatagt gatttataac aggtttctcc taattttatc tctaaatcca
     5401 aacgagacac cttattattg acaaaaagaa gttcataagt atttggattc actatataaa
     5461 tataattatc aatagaatca agaatttctt taagtactgg tttggatttc tcacatttat
     5521 ttgctaattt tatattatta tcacttatat tattttcaag ttcattttgg tagatacaat
     5581 tattataaag tgcatactta tcttttcctt tgctctttgc ataataaagt gctatgtctg
     5641 cttttttata aagttcttca aatacagttc catgttctgg tacaaatgaa attccaactg
     5701 ttccagatat tttatatgtc tttttatttt ccgtataaga atttctaaat atatcgcata
     5761 ttttttctgc tttctttttg gtattctcaa aagactgaat attttttaag aatactataa
     5821 attcatctcc accaattcga cctactatat catcttttct aaatataggt tttaggtgtt
     5881 tagaaacttc aaataatact ttatctccaa atacatgtcc taaattatca tttacagatt
     5941 taaaattatc tatatcaaca attaaaaatg catgcattcc taagcttgct tcgtcagagt
     6001 tcaagaaatc attaatcaaa ttttctgtag tacctttatt atataatcct gtaagagtat
     6061 ctctttcagc ttttaatttt aatttaattt cttcattttt ttgcttgtca atatctttaa
     6121 cataagcaag tgcttttata tcattcgtaa tagaatcttt tattaagtgc attgtgcaaa
     6181 tgacccaaat cattttattt tcttgattca ttcttcgata ttctaatttt atttcagtta
     6241 tatttttgtt atatgcagat aataaatttt tacacaaaaa tgtatttaaa aacacctctt
     6301 tattgtcttt gtgaataata ctgtttgata tagtttttac catttcagaa taattattgt
     6361 caggcttaat tccaaatggt ttttgccaac tttcatttcc tttgataaaa gtatcttttg
     6421 atacatttat ttcataggtt atcattgctt ccgacaatat agctgaatga tacttacctt
     6481 cttgattata tcttatctct gcttcctttt gtttgcttat atcttcctgt ataccaattg
     6541 ctcttacagg attaccatct tcatcaaaaa tagtagtcaa ggtaacttta ttccatatat
     6601 aattttcatc tggaagtttc atcttaacta cacaacttgc tttttttgct ccatttatta
     6661 cattttcata catgtttata aaatcatcac taaaatcttt atgaataact cctgattcta
     6721 ctagagaata tggaacgttt ttagtaattt tataaatatt atatttatca agctctttac
     6781 tagtatttaa aagtgacttt gtttttatat catactcaaa tatagtattt gatgtttgct
     6841 ttaatgcaat tctaaatctt tcttcattta ttttaagttt ttgttctgca ttttttaaat
     6901 ctgttatgtc atttagaatc caaatacctt tttcaatacc atcatcatca ataataagcc
     6961 ttccttttat agaaacccag ataatattac catctcttct tcttattcta tgttgatata
     7021 cagctattcc ttttgtgttt aattgatgtt gtatactttc aagagccttc tttctatctt
     7081 cataaaaaac aagattacaa gctttgtatt taagttcatt ttctaattgt tcacgtgtat
     7141 atccaatcat tgaaagataa ccagagttag tgtattttat agtaaaatca tcattaaatt
     7201 ctgttataat tataccacct aaaatatttt ctgatatcat ttcaagttct agattttttt
     7261 gttccatctc ttgttctaat tgttttgttc tagtaatatc tacagttaca caacaaaaat
     7321 attcttttcc atcaggttct gttataagtt ctcctttatc taaaacccat actgtattac
     7381 cattttttgt tataatccta tattctaatt caatagtttt atttttgtaa agttgtttag
     7441 aaatttcttt ttttattctt tcaacatcat ctttatgtat taaactataa aaatcattat
     7501 taaacaatgt ttttatttct tctcttgtat aaccaaacaa ctctataaat ccatcactta
     7561 aaaaattaat attgcatgtt tcacccaggg tacacttttg tactcctcca ggaatgctat
     7621 ttgtaataga tgagatttct ttgttagctt ttagtatatc tttctgagat ttattgtttt
     7681 tataaataat atatattata agcataataa acaataagaa tactttgaaa ataagagaaa
     7741 atgctaattg atttatttta tcaatgtttc ttcttacagt atattgtggt atcacagaaa
     7801 ttacatacca gtcatttaca ccaatgggtg tataagtagc ataacgattt tctcctttgt
     7861 attcatatga ggtaaagcca ctcttgttat tttttgtatc ttcatatatt tttttatagc
     7921 tataaccatc atcaaaagaa gcttttttaa atatatcata tatgttacta cttgacttaa
     7981 tcagtttgtt tgtatgtgaa gattttaaaa caaattgacc actagattca aatatatcta
     8041 tgtacccttc actattaaaa cttgttatat ctataaattc agctaattta tcattgtagt
     8101 aagaaccgta taaaacacca attattttgt tattcttatt aaatatagga gtagctataa
     8161 caaaagtatc ttttccatca attggagatt tcacaacttc agatatattg ctttctcctt
     8221 taatagcttt cttaaaatac tgatattttg acacattgat aattttgcca tcgctggtat
     8281 gacttattcc atcaggagat acaatagaga ttctttgaaa gtcagattct tttgatatgt
     8341 tagctaataa tggaactatt tttgagttat ctatagtatt aaaataagaa accatatttg
     8401 ccacactttt aataaaggat agagaactat ctaattttat ttttatagca ttggaacttt
     8461 gtagggatat ttcccttaaa ttaattttac tattttcttc ttcctggaca ttagttttaa
     8521 atgcaaaatc agaaaaagaa attattgtaa ataaaaatat taaaacaata ctcagtacat
     8581 tttttaacga ttttgctctt atcacttaat caccacctcc catttaagac tcgctgacta
     8641 tattttatag actaattttt caaattcttt aattggtaat ggttttgaaa aataatatcc
     8701 ttgaacaata tcacaatttg ctttttttaa catttctatt tgacttcttg tttcaattcc
     8761 ttcagcaaca acccttattc ctaattgctt aatgagagaa actatgcttt ttataacaat
     8821 ttgactctta tcaacatctt tatcatcaac aaaaaatcct ctatctaatt ttattacatt
     8881 taatggtata tttttaagca tatttacaga agaatatccc gagccaaaat catccattgc
     8941 aactataaaa ccaatatcct gtaggtttct actaacatta aatagagtat cagtatctcc
     9001 aaacatacta gattcagtaa tttcaatttc tattaaacta tatggtattt tatatctatc
     9061 acatatattt actatgtttt caataaaatc tttttttaaa aaatcaactc ttgagaagtt
     9121 tacagatata ggtaaaggtg aaattccttt atcaaataaa gaccggattt ctttacaagc
     9181 ttgttctaat atgtacatat caatttttct aataaatcca tttttttcaa atataggaat
     9241 aaaatcacct ggatatatga cttctttttc tgtatattgc catctgacta gggcttcact
     9301 tccacaaaat ttatctaatt taatattata ttttggttgt aaatacatta cgaattgccc
     9361 actttcaaga gcatattcca tttctttttc tatcttatct tcaagtaata atttctctct
     9421 taatttatca ttaaaaaatg caaaatttac atcactatta ttttttattg atgattttgc
     9481 aagtgaagct ctatcactat atacacttac agataaatcc ttatctttga tttcataaat
     9541 tccaaaagat aagtttacaa gttcattatt tgctataatt tttttaataa tatttattat
     9601 atcctctttt ttgttatatg ttaataatat attaaaatta tcagcactta cacgagcaaa
     9661 agtttcattg atattagtca attcatttaa agtatctgca atatctttta atattaaatt
     9721 gcccttttta tgtccatata tatcattaat tgctttaaat ttatcaatat cgaatatgac
     9781 cattgcatat ttattatttt gtgatgtttt ctttaataaa tttgtaacat ctaattcaaa
     9841 ctttctccag ttggaataac ctgttacaaa atccgtatat gctacttttt ctatagtttt
     9901 ttgactcttt gcttttgaat ataatatgta aattataata cataaaaata aaaatgaaag
     9961 tataatccat ccagtaattg taactgtcat tatgtcattt gtttcatatt ctaatactct
    10021 actaatatta cctatataaa agaatgtaaa tacactaaaa aagaatagta ctataaatgc
    10081 tattgatata ttctttatta ttaatctatt ttttttcaaa tagctacacc tcactctctt
    10141 tagcaattat aatataattg attcacttta ttttactatt aataagtatt ttttacaatt
    10201 aagttttata aattgttaat aaagagcatt taaataaata tcattatcat ttaaaagata
    10261 acaatagtta agggtacaaa tatatcaata gcatatttaa ttatattgaa tccatattta
    10321 ttaatacaat taaatacact atcgcttaca tttataccca tctatatata gcttaaatat
    10381 taatatttat ttttctaaag cttctcttct tattgatgat tttttaaaat actctatcaa
    10441 tttattttta tcattgttaa atatagcttc ttttactaaa tttacttctg attcaaagtt
    10501 ttctataact tttagtaaat tatctctatt tcctaaaaaa agttcactcc ataagtcttc
    10561 attcatattt gcaattctag ttaaatctct ataactatct ccaataaatt tacctgtatc
    10621 tctaccttct tcatcactat ttataagagc aactgccata acatgaggta actgagaagt
    10681 aaaagctata atctcatcat gtttttgtga agttaacttt tttactcttt taaatccaat
    10741 ctctaaaatt aaattttcaa ctaactctaa attttttatg ttgtttctgc cagttggagt
    10801 aattatataa tttgcgccat taaaaacttg ttcacttgca aaatcaatac cctttttttc
    10861 cctacctgcc attggatgtc caaatataaa atctatatca tctggaataa tttgcaacac
    10921 atcattgata agtgtttctt ttattcctgt agtatcagta attatagtcc cttttttaaa
    10981 aaagttttta ttattattta gaaaatctac aactaatcta ggatatatag atagtataat
    11041 taaatcagct tttttaaata actctttacc tgtagtacat ccttttttta tgatttttgc
    11101 tttttctgct ttttttaaag tttctaaatc tacatctact ccaaatacat tttcatatcc
    11161 agcttttttt aatgctttag caaaagagcc tcctataact cctagtccta caataacaat
    11221 attcattatt tttatttccc cctagattat ttttctttaa agtcaaattt aaaattttca
    11281 ctgactgcat ttgtaatatt atatacagca ttatttaatg atttatcatt taaaatctct
    11341 atatctgaaa attttttata aagatttatt ctctcattat aaagattata caatctatct
    11401 tttccgtttt ttaataatgg tcttgaatta atatttatat cttccaaaat cttttgtata
    11461 ggtctatcaa taaaaattat tatacctgtt tcttttagta tttccatatt aacatccttt
    11521 tttattacac ctccacctgt agatatcact gttttatcac ttatagaaag ttctctacat
    11581 gcttgagtct catagttcct aaagatatct tctccttctg aaaacaactc tgctatactt
    11641 ttttgagata tttgaattat ataatcatcc atatcacaga aactacaatt atattctttg
    11701 gccaaaagtt taccaattgt agtttttcca ctacctggca tacctattaa tatcaatttt
    11761 tcttttgttt tatttatcat gacaatctcc cttacaaaaa ttctaatttt agttcattat
    11821 atataacatc taaaatacta ttatctattt tagtatcttg ccaaatttcc tgagatttta
    11881 ttgcttgccc taccagcata tataatccat cacaagtttt tttacccata ctattaccaa
    11941 ttcttaaaaa ctctgtttct ccaggattgt aaattaaatc tattaatata tcaaaatttt
    12001 gtataataga ttttgacact ggtgaaattc ctacattagg gtacatacct actggtgtag
    12061 catttaatat tatatctcct tttatatgtt taagttcttc ataatctatt aatatagctt
    12121 tactatttaa taatttttta tcatcctttt ttctagttga aacataaagc ttttctatac
    12181 cagaatcaat aaaatatgta agagcagctt tagaagcacc tccagttcct aatataactg
    12241 ctacttttcc ttgaacatca atattagcca ttttaaacat actatcaaga ccaaagtaat
    12301 ctgtattgta accatataac atattttctc tgagtaaaat tgtattaact gctcctatac
    12361 gttttgcctc tggtgatata aaatcaagat atttcataat acgctcctta tatggtacag
    12421 taacatttac tccttgtatt cctaataatt ttatagcacc gtctagctta gatatatctt
    12481 cttttggaat ttcaaagttt ttataagcac tttcaatatt caatatctcg aatattctct
    12541 tgtggatttg aggtgaaaca ctgtgagaga gtttttcacc tactagaccg aaaaaattca
    12601 tacttacacc tcctaataat tataaattaa ataaaaattt atattatcta ttcagtttta
    12661 tttttatata ctcccataaa tttaaaatat gctgtattat gttctatcaa ttctaataag
    12721 tcgtacactt tagaattgta tatagaacac tcaaaatcta tataaaagaa atatctccat
    12781 gaggcatttt tcataggtct tgactctatt tttgtcatat ttatattatt ttcagcaaag
    12841 tatccaagta ctttataaag ttttcctgct tcatgttcaa cagaaaatac tacactcatt
    12901 ttgtttgatt cttcttcaat gtgtatttga ttactgatta ctacaaatct tgtatagttg
    12961 tttgcaatat catttataca aggacttatt atttctaatt cataaatact tgcagctttt
    13021 ttgcttgcaa ttgctgcttt tgttttatct tgaagttttc ttacaagttc tgcacttgtt
    13081 gctgtgttat taaatggtat caatttccat gtactatttt gttttaaaaa ttcactacat
    13141 tgtcctatac cttgtggatg agaatatatt tcttttatat cttcaagttt tgtgtctttt
    13201 atccctatta aattttggtc tatttttata cattcttctc caactatata aaaaccgtac
    13261 tttctcaaaa ggtcataagt ttcagagata gctcctgtag atgaattttc tatggggatt
    13321 attccatagt ttatttcttt atttttaagt gctatgaaaa catcttcaaa ttctttaaat
    13381 tgtttttttg atattgtttt gttgaagaat ttatttagtg cttcttctgt aaatgaacct
    13441 gattttcctg gaaatcctac taggatattt tttttattta attcatcttt taaagactta
    13501 tatttattta catttgacaa atcaaatttc ttatttgatg aatctaaact aaactcgatt
    13561 ttctgctcac catcattatc tatcaatctt ttttgtaatc ctctactaat ttccattata
    13621 gaattaaaaa actgtaccgt ttcttgtgag tatgctttat tatctaaatt atctatggct
    13681 ctacttagta ctttttcttc tctatctttt tgaagtatag tagtgttatt tttcatttta
    13741 tgtctggcaa tttcaagaac tgtattcatt ctcttttcaa ataattgaat aatctcttta
    13801 tctatattgt caatctcatt tctatatctc gttaaatcct ccattattat cgtcctttca
    13861 tcaaatcaaa aattccaatg gctgttactg cttctattac aggtatagcc ctttgaacta
    13921 tacaagggtc atgtctgcct ttaatatata aaatatcgtc ctttttatct tttatattaa
    13981 ctgtgttttg ttgtctgcta attgaaggag ttggcttaat tgccactttg aaaattattg
    14041 gcattccagt agtaatccct cctataatcc ctccattgtt atttgttttg ctcttaactt
    14101 gatttccttc ataatacatt tcatcattac tttgagagcc atacatatct gcaagttcaa
    14161 aacctaaacc aaactctaca ccttttacgg caggaacaga aaacaataaa tgtgataaag
    14221 tagattcaac agagtcaaaa aatggatttc caagccctac attaatacct actactgcac
    14281 actcaattat accaccaact gaatctcttt gattttttgc atctataatc gtatttttca
    14341 tggcttcttc ctttgataaa tcaagtaaag gtagttccaa agtttgcaaa tttgataact
    14401 gttgttttga aatatttaca taatcaaaac tcatatcttc tatattttta atttttttta
    14461 tgtgtgctcc tatctctatt cctttttgtg ataaaatttg tttacatata gccccacaaa
    14521 atactaatgg agctgttatc ctacctgaaa aatgacctcc ccctctatag tcattaaatc
    14581 cagaatatct cacattacct gtgaaatctg catgtcctgg tctcattaaa tttttaagct
    14641 ctccataatc ttttgaacga gtatcactat ttcttatgat tgcacataat ggcgttcctg
    14701 tagttcttcc attaaaatat ccacttagaa tttctggtat atcactttca tttcttgaag
    14761 tagatataga attttttcca ggtgctcttc tcttcatttc tttatcaatc ttatcaaggt
    14821 caagttcaat ccccgaagga agaccatcta tatttatacc tatagcattt ccatgagatt
    14881 ctccaaaaat tgatactttt aaattatttc cccatattcc actcatcaat tcgacctcct
    14941 aacatcttaa aatctttaaa aaaatgtgga taagattttg atacacactc aaaatctttt
    15001 aatattattg gtttatcaca tctacaagaa gcaattgcaa gtgtcatagc aattctatgg
    15061 tctttgtgac tccacacctc tactccacca tttaacttgc ttactccttc gattatcaaa
    15121 ctatcctctt tctcctctat atttgctcca agtttactta actcaacatt tattgcatga
    15181 agtctatcac attctttaat tctaagccta ccagcattaa ttattgtagt cctccctata
    15241 cttaaggatg ctactacaga taatacaggt attatatctg ggcattgaga tgcatctatc
    15301 aaagtactat ttaaaccatt tgttattcct tttattttgt tgccttctct taatatttcc
    15361 attcccatcc tagataaaat ttcaactact tcactatccc cttgaagaga atcttcttta
    15421 aggtctaata tgctaatatc ttctcctata gcatctgctg ataaataaaa tgctccttga
    15481 gaataatctc cttctacttt ataatctctc gctttatacg tttgatttcc ttttataata
    15541 aattctttat agttattgtt tataatttct accccaaaat cttttatcgt tgaaagagtt
    15601 aaatctaaat atcctttaga ttctagctca gtagtaatta ttatctttga atcactctct
    15661 agtgtaggca atataaacaa tagacctgtg ataaactgag aacttatatt tccttttact
    15721 ctaaattcat ctggtttaag cttacctgat ataatcaaat ctaactgatt ttctttgtaa
    15781 ctatatttta tattctgtct gtcaaatatc tcataataag tatcaagtgg tctttttcca
    15841 agattacctc tacctataaa cctttttaca ccatcaaatg ctaaagatat aggtactaaa
    15901 aatcttaatg tagagcctga ttcattacaa tctattgtca atttaggttg attcaactga
    15961 ttctctctat ttaaaatacc ttctttagaa aaaattcccg aaatttctaa aatatcttct
    16021 ttcttttcta taatagctcc taatgatgtc attgctctta tagtagctat tatatcatca
    16081 gaaaaatcta tattagaaat tctacttttt ccattgctta gagatgaaca aataactgcc
    16141 ctatgtgcca tactttttga tggtggtatt tttacatctc cagataattt acttggatat
    16201 atttttagac ttcccattat cttacctcct gaaaaaattt agaagtagta ttatatataa
    16261 tactctctcc aatatctttt aaaagtatta tttttaatgt atcacctaga ttttttttat
    16321 caagagatat agcatctact atagatttat tatctcttaa ttcaatctca taaggcaaat
    16381 tataattcat taaaattgat tttataagtt ctgatgtacc tttttttgta attcctatct
    16441 tttcactttc taaagttatc ttatacattc ctatggcaac agcttctcca tgggtataac
    16501 ccgtaaaatt gtagtatttc tcaatagcat gacctaatgt atggccaaag tttagaagca
    16561 ttctatctcc aaagtctttt tcatcagctt caactacagt ttttttaata aagcaacatt
    16621 tatgtataat gtattctata tgttgcatta cttcttctct attttttaaa cttaaaagtt
    16681 tattgaaaaa agttttgtct tttatacatc catattttat aacttctgcc attccatctc
    16741 tatagaattt ttctggaagt gataataata catttgggtc gataaacaca gcttttggtt
    16801 ggtaaaagct tcccactaaa tttttgcctc tctcaaggtc aactccaact tttccaccta
    16861 cagaactatc aacttgtgct aataaagatg ttggaatttg aataaaactt atacctctta
    16921 aaaaagttgc tgctgcaaat ccacctaaat cacctattac ccctcctcct aaagtaataa
    16981 tcaaatcttt tctagttaac ttaaagtcaa gtaaactatc gtatatttta ggaagagtat
    17041 taaatgattt tgtttcttca ccagatttta atattaaaga tttaatttca aatccagatt
    17101 cacttaaatt attttttact ttatccaagt ataattttgc tatattttca tcagttacaa
    17161 taaaaatctt atttccaaaa tatatttttt ttatttcttc tcctatattc tttagtatat
    17221 tttttttaat aaatatattg tagctatttt cttttaaatt tactaatagt ttttcttcca
    17281 cgtttaacca tccttttata gattatattt ctctaccaac tgctgatgcg atagtcttta
    17341 attcacttat caattcatca tattcatttg gcttaatcga ttgttttcca tcacaaagtg
    17401 catgtgctgg gtcattatga acttctatta ttagaccatc tgctccaact gcaattgctg
    17461 ccttagataa agagtcaacc atccatgatt tacctgctgc atgacttggg tcaactataa
    17521 ctggtaaatg actaagtttc ttcacagcta aaattgcact taaatccaaa gtatttctag
    17581 tatatgtttc aaaagttctt atacctcttt cacaaagaac tacattttca tttcctccag
    17641 ccattatata ctctgctgac attagccact cttcaattgt agcagataat cctcttttta
    17701 aaagtattgt tttatttgtt ttacctaatt cttttaataa atcaaagttc tgcatgtttc
    17761 tagctccaac ttgaattaca tctacatttt cttcaaatat atctatatct tgtgttgaca
    17821 ttatttctgt aactatagga agccctgttt tttcttttgc tttttttaat aaatccaatc
    17881 catcatattt caatccttgg aaagcgtatg gtgatgttct tggtttgaat gctccacctc
    17941 tcaaaaatcc tgcacctgat tttttcacat ctttagctat agatactatt tggtcttctg
    18001 tttcaacaga acaaggccca gctatcatag caattttctt tgaaccaatt tccattccat
    18061 taacatttat gattgaaggt tctggatgaa attttctatt ggccttttta aaaggctcta
    18121 ctacatgcat aactttttca actatcttat ttgcttctat tcttttggca tctattttac
    18181 tagtatctcc aactaaacct aaaacagtta attctgttcc atctatggga ttaacttcta
    18241 ctccttctct cccaatagcc tcaattaatt ttttaacttc atttttatct gctcccattt
    18301 ttaatactac tatcattttt actacctccc aatattataa ttttatattt aaaataaaaa
    18361 agagaactca ctaatgagtt ctctaaaata atctatcaag cactaaatct tatataaata
    18421 tataaaataa tcatttttat aaaatttact tatgctcaaa atcactaaaa taaggcctca
    18481 ccagaaagga agtacatata tttttgtaaa agaaatagcc agcgctaaaa taaaagcccc
    18541 agaacataaa gtaaaaaaag ctataaagtt ttgctgttag ttcttttaat attcctttca
    18601 tagtattatc ctccattttt ataatttatt attcttcttg tttaataatt ataacaaaga
    18661 aaatataatt gtcaatattt tctataaata ttttcaattt aaaatatttt tttatttact
    18721 taaatcagct tctcaattga tgtattttta taaaatcaat taaacatcat ctgtaattta
    18781 caataatttt tttattttaa atattataat tccaataaaa attatacata gcataaaaaa
    18841 taaagttgct ggatatccat aaatccagtt taattctggc atatatctaa agttcattcc
    18901 atatatacca gttataattg taagtggtgc tgatatggct gttaacaagg ttaattttgt
    18961 aattaagtta tttgttttct cacctactct ggaagaataa atatctaata atttatctgc
    19021 taactctcgt gttgacaatg cgaaattgta taatttgtca attccaaaat ctataccttg
    19081 aaaattttct aaattatatt tttttacatt tgaatatttt aaatatctta tattttcctt
    19141 aactattcta tccccaatat acaaaagagg tctagtattc ttaactataa ttcttgttaa
    19201 tcctcttaca tcacttattc tagatacatg attcttatta atgttgtcca tcatttcatc
    19261 ttctatttga agaattaact cttcaacttt ttccaaagac tcaaaaccat tttttataac
    19321 ttctctaaga atcaagtagt ttatcttaaa tagattaata actggatttt tttcaagttg
    19381 agagttcttc aaaataatat tttttacaaa ttcaaataaa aagtgttctt cattcactac
    19441 aaccaatata aaattatctg ataaatacat atttacttct tctataacag cttctccatc
    19501 tcttagttca aatgtattta ggcttaaaaa atcgtaatta tcaaataaat caagtttggt
    19561 tatttcatca aatttaagac aatcattaaa agttatttca tctatatcca aagtttcctt
    19621 aagcaatttt aaattttttg gagcacttaa tataagataa gaattttctg ctacataaaa
    19681 tttatctcta aaatcttcaa tagtctctct tgtatttaaa ttcagtatat acatacatct
    19741 actcctaaat ttcaatttac attctaaaaa ttttttataa tccttactgt aattatttta
    19801 cactatttta acaattatta tttaatattt acattaaatt tattataagg taagtgttaa
    19861 atacatttat tatttaacca aagtttttat tacagattac actatatact tattttaaaa
    19921 catatttcat attattgata ctaaaaagct aaaaaataaa atgcatgtgc taaactgaca
    19981 acaagcacat acacttcatt cttattttaa ctaaactata taattttaat atatattaat
    20041 aagcacataa tctaataatt aatatattac aactattcat catttaatct gtatcctaca
    20101 ccaacttctg taattatata tcttgggttt gcaggttgtt tttctatttt tcgtcttaat
    20161 gttgccataa agacccttaa agacttagtc tcattcccta taacactacc ccatatttct
    20221 tttattataa aattatgagt aagcactttt ccatgatgct ttgcaagtaa tgaaagaata
    20281 ttatattcaa taggtgtcaa atggacttca acatcatcaa caataacttt tcttttatca
    20341 aaatcaatca ataatttatc tacttcaaac gtagatttaa catcatcttt ttgttgagct
    20401 tctacttgtg aatgtctaaa tgcgactctt actcttgcta ataactctac tatggagaat
    20461 ggttttgtta agtaatcatc tgcccctgca tcaaatactt ctaccttatc tctatcttgc
    20521 tctctagctg aaactacaat tattggtata tttgaaaatt gtctgatagc ttttattacc
    20581 tctattccat ccatatcttc taagcctaaa tctaaaagaa caacatctgg agaatatgag
    20641 acagcaatag atataccttc ttttccagtt atagcctcct tgacatcata atcctgtgtt
    20701 aaaagtgcag tagatataaa ttttcttatt gtcttatcat cctctactaa caatataagt
    20761 tttttactca tttcttcctc caaaactaat tttaaaattt aaaactattc attttcctta
    20821 ggcaaactaa acttaaaagt tgaaccctta tctttattgt tttctaccat aatttcacct
    20881 ttatgagctt gaattataga tttacaaatt gataatccaa gtccaacacc ctttctggaa
    20941 tctttagctc cttcttctcc agtaaaaaat ctgtcaaaga tatgcttttt aagttccttt
    21001 gatataccag gaccattatc agatacttca aaccaaacat aatcatcttt ctcgtataca
    21061 tttatctcta tcttacaatc ttcctttgaa tatttcaaag aattatctat taaattaatt
    21121 aaaacttgtt ctattaattt tgcatccata ggaacaaata atatctgttc tggtatatta
    21181 atatctataa gtgcattttc aattcttttt tttatttttt gtaacgcttc tgatataatt
    21241 tcctcaacca gttcagaatg ttttttaact tccaatttcc cttcatctat cttggtcata
    21301 ctaagtaaat tttcaacaag tcttattaac cactgagtat cttcatatac accactaagt
    21361 aattcatcta ttatatcttg acctatagta cccttgtttt ttataattgt acttactgca
    21421 ccagatattc ctgctagtgg agttctaagg tcatgcgaaa ctgctcttaa aagattactt
    21481 cttaatcttt ccctttcaat ctcaagattg gtattttctt tagcttctga caagatttct
    21541 ctatcaagcg ctattgccat ttgagcaatt attgtttcaa ttaagcttat atcttctgta
    21601 tccaattttg aatcaataca tgatattcct actactccta aagttaaatt cattcctaca
    21661 attggtatat agtatccctt tgaaccagga agtgtatccg tgtcatttcc tgccactgtt
    21721 gaattggata aaacccaact tgcaacagct ttttcatctt cactatccaa atcattttta
    21781 tccttattat tagtattttt tttgtaaagt tttgatttat tatgcttatc tacatttaaa
    21841 taacaaatta catctctact taagctaaga gacaaaagtt ctataccttt gtttactata
    21901 tcatctttac ttgataatct taaaaatccc ttaattattt gatatatcat ttttgtattt
    21961 tcttctcttt tagcagcagt ttctgcttca tgctgtatct tagacgtaag agtgctagtt
    22021 attataccta ctattgagaa tacaaccagt gttactatat agtttgaatc tgctatagat
    22081 aatgtatata atggtgctgt aaaaaaataa tttaaaagaa ctatattaaa tacagaactt
    22141 attatacctg tactataccc tttagtccac atagaaacta aaactacacc caacatatat
    22201 attaacaata tattttctct tataaatccc atgctttgta ctgcatatgc taaaatactt
    22261 atcatcaaag taataaatat aagttttata aaatcaactt tgctaatttt aaatttagat
    22321 tttattgatg atttatcctt tttaggtttg tatttaacat ctgaggcaaa tttatatgga
    22381 attatatgta tatctatata atcaacttca tcaattaatt tgtcaactat atctttttta
    22441 aatcttttac taaatctttt attacttgaa tgatttctac ctattacaat ttttgttaca
    22501 tttctcagtt ttgcaattct aagtatttgc tctattatat tttcaccatg taaaacaatt
    22561 atttctccac ctaatttctt agctaatttt aggttatttt gtagttgttt ttgttcatca
    22621 aatggaagtc ttgctgtgtt tggagtttca acatataaag ctatccattt tgcaaatgta
    22681 ctgtctgata ttctggatgc agttctaata acttttgctg aagatggaga tggacttata
    22741 catgcaagaa gtgtatctga tgtaggtatt acagttacat caccctttga tagtctggtc
    22801 atttgaactt ctttattaac tctctctgct gtttttctaa gtgcgatttc tctaagtgca
    22861 accaggttat cttttataaa aaaattattt tttgctctag tagcttgttc tttcttatat
    22921 atcttacctt cattgaacct ttcaagaagt acatcaggtt caacatctat aagttctaac
    22981 tgtgtatttg tatccaaaaa cttatctggt atagtctctt taactgatac gtttgtaatt
    23041 acctcaacaa tgtcatttag actttctaaa tgttgtacat ttacagtagt gtagacattt
    23101 atgcctgcta acagcaattc ttctatatct tgccatcttt ttgtatgcct taacccagca
    23161 acattagaat gggcaaattc atctactaat ataacttctg gttttcttgc taaagcagca
    23221 tctaaatcaa attcttttag ttttatagat ttatattcta tctcctttac aggaagaatt
    23281 tccaagccat caagtagagc caatgtttct ggtctagtgt gtggttcaat atatccaact
    23341 actacatcta cacccacttt ttttaaattt tgtgcagatt ccaacataga gtaggttttt
    23401 cctactccag ctgcataacc aaagaatatt tttaattgac ctctacttag tttttcttct
    23461 tcatttttaa ttttttctaa tagaatatca ggattaggtc tatccattaa aaaacctcct
    23521 tacaccattc attactatta taatatcaat tccgatataa aattgtagtt aagatgtatt
    23581 tttaatgatt aagattatgt aaagatttaa atatgaattt aaaaaattta gttgtaaaat
    23641 aaaaatgcct aacaataggc atttttatct tgtgtataat cattttagtt taagttaagt
    23701 atttccataa actcatcacc tgtaatagtt tctttttcta ataaataatt agcaagttca
    23761 tgcaatttat caatattatt ttctaagatt tcaattgcat ttttgtgaca agcctttact
    23821 attttaaata cctcttcatc tattttagca gcagtgtcgg aagaacaagc aagagaagta
    23881 tctcctccta aatattgatt gcttacagtt tcaagagcga ccatatcaaa ctcttcactc
    23941 ataccaaggc gagtaaccat agctcttgca attttagtag cttgttctat atcatttgat
    24001 gctcctgatg tacaagtgtt aaagataacc tcttcagcag cacgaccacc tgtaaaagta
    24061 gttattttat caattgcttc ttctttagac ataagtacac tctcatcttc agcaacttgc
    24121 atagtatatc ccaatgctcc tgaagttcta ggtattatag taatcttatg gacaggtgca
    24181 gaatgttttt gcatagcagc aacaagtgca tgacctattt catgatatgc tataattttc
    24241 ttttccttat ctgaaattac agctccttta cgttgatacc cagcaattac agtttcaaca
    24301 gattcatcaa aatcctgttg ttctactata tctctgccag acttaactgc taaaagtgct
    24361 gcttcgttta caatatttgc aagttctgca cctgatgctc ctgatgtaga ccttgcaatt
    24421 gcattataat ctatatctga gcttacattt acattttgag catgtacctt taatatagct
    24481 tcacgtcctc caaggtctgg aagttcaact ggtatacgtc tatcaaatct tcctggacga
    24541 agtaatgctt tgtcaagact gtcaggtcta tttgtagcag caagtattac tactccaatt
    24601 ccaccatcaa aaccatccat ctcacttaac aactgattta aagtttgttc acgttcatca
    24661 tttccaccta atccacttcc attatcacgc tttttaccaa tagtatcaat ctcatctata
    24721 aacactatac atggagcttt ttcttttgct tgtttaaata aatcacgaat cctagaagct
    24781 cccattccta caaacatctc aacaaactca gaaccagata ttgagaaaaa tggtacattt
    24841 gcttcacctg ctacagcttt tgcaaggagt gtttttcctg tacctggagg tcctactaaa
    24901 agtgctcctt taggcatttg tgctcctatc tttttatact tttctggatt gtgtaagaaa
    24961 tctactattt cagttaaagc ttcttttgcc tcatcttgac cagctacatc attaaatctt
    25021 tttccacttt gagcagatac atacattttg gcgttgcttt ttccaaattg catagcatta
    25081 ccacccattc gcttttgcat agacttcatc attaagttac caaatagcat aagaatacct
    25141 ataggtatta tccatgtaag taaaatactt aaaagaggtg aattttcctt aggaataacc
    25201 ttagtaaatt ctacttttgc attttttagt ctatctacca attctgggtc atccattcta
    25261 ccagttatgt atatattctt atccttgcta ctagatggat ttattgctat tctattattt
    25321 tctatctcaa cagatttaac ctttcctgta tccaacattg tgagaaactt tccatagtca
    25381 atctcaacca tttttttatc cattaaactt ggaaatatta aagcatttaa aaacattact
    25441 actattatta taactgtata ataatagagt gctgaccttt tttggggttt atgttcatcc
    25501 ataattttat tctcctttaa attattttta tgttatttgc aatcaatgta agttaaaagt
    25561 ttatttaatt tctctaaacc ttctattata tcttccatat cttgcaattt ttcactggtc
    25621 atattttcaa aataactatc aaaaatacta cttgaaatat gttcttgtat atccaatgat
    25681 ttatttgtaa cttttacttt tacaattcgt tggtcttcta tatctcttat gcgatttaaa
    25741 aagccatttt tttcaagcct ttgacagatt actgatacat tactatttgt catgtttaag
    25801 ttcttcccta aatcgcttat ttttttctct ccatgttcac ataactcaga taaaatcaaa
    25861 gcttgcactg ctgtaatacc aagtggctga taaaaatcac ttaataaagt tgtaaatttc
    25921 atatttatat ctctagtcaa ctctagtagc tctttcttaa aactcatctc atccatacaa
    25981 acgctcccta cgaaaatact ttatttcaaa tttaggctga agtgcatact gtaaataaat
    26041 acttaatact ataatgttta atgtattaaa tattatagta ttaagtattt attgtcaatt
    26101 acaatattgt aacttcaatt ggaattttgt tatcttattg atatttaatt ttatacagga
    26161 tatttttaat aaaatttaat aaaaaatcga tatatatatt ttacactgat ttctcccttt
    26221 ttaatctctt ttaaaagtta aaatacaaat ttattgatac tataaaggtt tagtctccca
    26281 ttatcgcacc atatataaaa ttaaaaccac ctctatctac atacacctaa agtgattttc
    26341 ttaaaatata aaactactat tataaggtta aatttaaaaa aaataaggta taaagtatca
    26401 ttaagattaa ctttatacct ataaatttct atattttttt agttaacatt tatatactct
    26461 taaaatttat tgtatcaaat agttttttat ccaatttcta aacaacaatt ctgaatgttt
    26521 tttccatttt acagttggct cttctgaagg gtcatcatta atatagtaat tttgaggtat
    26581 agatatttta tctcctttat ttatatccct cttatattct ttatcaagag tgtatttgtc
    26641 atactcgcta tgacccatta cgaacacatc tcttgaattt gtaattatat agggaccagc
    26701 ttctttagaa tgactcacaa tgctcaactc tttattctct ttcaaagctt caatatttac
    26761 agtagtatgt cttgaatgtg gagcaaaaaa ttcattttca aatccatcta ctattttact
    26821 atctttatct accttatgct taaatactcc aaaacatttt tgacttagtg gaagtttttt
    26881 tacattgtag tatttgtaaa gacttgcttg agctgcccaa catatatata atgtagattt
    26941 agtttttctc ttagaataat ccataatttc ttctagttca ttccaataat caacttcttc
    27001 aaactccatt tgttcaactg gagctcctgt tattataaag ccatcaaaat actctccttt
    27061 ggcttcatca aatgttttat aaaattcttt aacatattca ttacaacttc ttttactttc
    27121 tcttgttgaa attttaataa attcaacaga cacgttgaat cctgttttat ctatccttct
    27181 tagtaaatct aattctgtat ctatctttat aggcattaga tttagtatag ctattttagt
    27241 gtctatgttt tcttcatact ttaattcctt tttaaaatct tctttataaa ttacatctat
    27301 tccttcatct aataatttat taataacagg taatcccttt ggtagtatta aagccatatc
    27361 ttttcacttc tctttctctt agaatattag aaatttaaag cttgatttaa atcagctatt
    27421 aaatcctcta cattttctat tccaacagat aatcttatta gtgaaggcaa tactccagat
    27481 gcaattaatt gttcttctgt taattgtcta tgagtagttg aagctggatg tataacacaa
    27541 gttcttacat ctgaaacatg tgtaaccaaa gatgctatct gtaatttttc cacaaatttt
    27601 ttggcatttt ctaacccacc tcttactcca aatgataaaa caccactaca tcctcttgat
    27661 aaatactttt tggcattctc ataatacttg ttatcttcaa gtcctgggta attaatccaa
    27721 tctacatttt catgtttttc taaaaatcta gctattttaa gtgcattttc actatgtctc
    27781 tccattctaa gatgtagtgt ttcaacattt aaattagtaa gatacgcatt gaatgggctt
    27841 aaacaatttc catagtctct aagcaactga actcttgctt tagttgcata tgcggcattt
    27901 ccaaattttt gagtatagct tattccatga tatgttgggt ctggctcaac taattctgga
    27961 aattttccat tatcccagtt aaagtttcca ccatccacta taattcctcc aacacttgaa
    28021 gcatgcccat ctaaatattt tgtggtagaa tgagtaacta tatttgctcc atactttaaa
    28081 gggttacaaa gcactggaga tgctaatgaa ttatcaacaa taaatggaac attaattctc
    28141 ttagctacat ttgctatttt ttcaaaatct atgacttcaa gagtcggatt tgcaagtgtt
    28201 tctgcaaata caaccttagt attttccttt gcaagttcta ctatctcatc ttcacttgaa
    28261 tctaagtcaa acgatattaa atctatacca aattttttaa gactaggtcc tagtaaattg
    28321 aatgttcctc catatacttt tgaagaacaa agtatactat ctcctgattt acatatattt
    28381 aaaactgcca acatatttgc agattgccct gatgatacag ctacagaaga tactccaccc
    28441 tctagtaaac ttattttttc ttcaaaagct tgaatagtag gattgcttat tcttgaatat
    28501 atatgtccat caacttttaa atcaaataat tcagcaagtt ggtctatact gctatattta
    28561 aaagttgtac tttgatataa aggtaatact cttggttctc cattacctgg tttataattt
    28621 ccttgcacac atattgtttc tttattatac attcttgtat cccccttaga ttttagtaaa
    28681 aaaaattaaa ctatttatca gatgagagtt aaaataaata aagcctgagt atataactca
    28741 ggctctgtat aagcttaatt atactcatct ctcagctttc gccgcaggat ttagcaccaa
    28801 tgtatataaa tatactggtt gccgggtttc atagggccag tccctccacc tctctggata
    28861 agatttattt aatttttcta tgttttatat cttatatatt tttcctaatt ttgtcaacta
    28921 tttttttctt ttttttcttt taataatttt cccaacctct acttgaatta cagacattat
    28981 ggctaaaaca atcgttataa gccactctaa gttatttaaa actgttaagt taaaagcatt
    29041 tctaaaaaca ggtacaaaca gtattactgc tattaaaaat gcagaagtta aaaatgagat
    29101 taaaagaaat ggatttttac cattatttct attaaacatt gaatctgtat ttgaacgctg
    29161 actaaatgct cgtaacatct gtgaaaatgc taatacacaa aatgccattg tttgacctac
    29221 agaatgattt tctatattaa gaccaatttt atatgctaaa atagtcatta ttgccacaaa
    29281 tataccttgg gtaacaactc tttgtataag atttttttca aacaaagtgg ttgatttcac
    29341 tggctggtgc ttcattatat ttctgcttgg tgggtcaact ccaagagcaa gggctggaag
    29401 cgtagcagtt gcaaggttta cccatagaat atgaacagca agaagcggag tatcccaatt
    29461 taataatatt gctacaaaca acgtaagtat ctctgctata ttaccaacaa gcaaaaattg
    29521 tataactttt tgtatgtttc tatatacacg tctaccttct tttattgcat aagcaattgt
    29581 tgtaaaacta tcgtccatta aaatcatgtc agaagaatct tttgcaacat ctgtaccact
    29641 tatacccatg gctataccaa tatcagcaga ctttaaggca ggagagtcat ttacaccatc
    29701 cccagtcatt gctggtactt ctccaattct ttttagagat tgtataattc taagtttatc
    29761 aaacggggac acacgagcaa atactgtagt atttttaaca gcttcatcta gttcatcatc
    29821 actaaaatta tcaagttcat caccagatat cacagtattt tcttcactcc atattcctaa
    29881 ctcatgagcg attgttgtgg cagtaacttt atggtcacct gttatcatta tggttcttat
    29941 acctgcttct ttacaagtac ttactgcatc aattacctca gttcttggtg ggtcaatcat
    30001 accaaccata cctataaatg tcaaatcata ttctatattt tcactatctt cttttggtat
    30061 agatgaaatc tcacgcttag caaaaccaag tacacgcaat gcatctttag acatgttaag
    30121 acataattcg tgtatattct ttttatccac ttcggtaatg tttctttcac cttttgaagt
    30181 taatatttta gtacataggt cgagaatttc gtctactgct cctttagtat aagcaataat
    30241 cttttcatct atattatgaa cagttgtcat acattttcta tctgaatcaa aaggctgttc
    30301 aaatacacgt ggatattctt cttcaagagc ttcttgatct attccaaatt ttttggccat
    30361 aaaaattaat gcgccctctg ttgggtcacc taatatttca tctgttttat ctggattaat
    30421 acatgcatca ttacaaagtg caccagcata aacaatctct ttatacacta atggatgttt
    30481 ttcagatgca gtatctattg atgtcacttt tccagattca aaatctccat tgacagcaat
    30541 atgtgtaaca gtcattttat ttaaggttaa tgtaccagtt ttatcacaac atattactgt
    30601 agctcctcct aatgtttcaa cagcaggtaa ttttctaatt aaagcatttc ttttagccat
    30661 acgctgtacg cctaaagcca ttacaatagt agctgttgca ggtaatccct ctggaataat
    30721 tgatatggca agggaaattg caatcataaa cattggcaag agaggtctct gataaaatgc
    30781 acctattgca aatataacaa cgcatacaat aatacctatg acacttaatg tctttccaac
    30841 agtattaagt ttacgtttta taggagtatc aaaatcatct tgtgaatcta aaagatgtgc
    30901 tatgttaccc acttctgtat ccataccagt tgctactaca acacctacac ctctaccata
    30961 tgtgactatt gaagatgtat acgccatatt gctacggtct cctaatacac attctttagg
    31021 taagattgta tctgcatctt tttctgatgg aacagattct cctgttaaag aagcctcttg
    31081 aatttttaaa cttgatgttt ctattaaacg aaggtctgct ggaaccatag ctccatcttc
    31141 taaaaaaaca atatctccta ctacaagatt acttgctgga ataacatttt cttcaccatc
    31201 acgcataaca cgagctgtag gtgagttcat attttttaat gcttctaatg atgattctgc
    31261 ttttttctct tgaacaattc caataactgc atttactgtt acaattatta gtattacaaa
    31321 agcttctgta aattcgccaa atatcaaaga taatattgct gctcctatta gtatcataac
    31381 catagcatct gtaatttgac tccaaagcat ttgtaaagtt gttctaggtg gtttttttct
    31441 aagttcattt aagccattct ctttaagccg tttttccgcc tcggtatcgg tgagcccctc
    31501 ctctgatgaa tctagacttt ctaaaatttc atgcgttgct cttgaatgcc aaggcatctg
    31561 tttgtgattt tccattttgg aagaccactc ctttcttttt ttaatttaag aataattata
    31621 ttttaaaatc aagttcaatt caatacacac taagtatatt ttacctaaat ttaacaatgc
    31681 catttaatat ttaacaaaac ttctactctt ttacaaatat gtattcacta ttgttagata
    31741 agttttcttt atacttataa ccttctaaat taaacttttt cagttcttca atatctctta
    31801 tcctattttt tacaatgtat tttgtcatca tacctctagc tttctttgct tgtgtagatt
    31861 ccactttcaa tatgccattt ttatttactt taaaagtaca tgtaatatat gtatcactat
    31921 ctttaataaa tttttctatg gacttgctgt attcatttga tgaaagatta atgatagctg
    31981 ttttttcttt gattagttct ttatatatac gatttcccca atattcgtat aaattcttaa
    32041 actcacctac tcgtaatttt gtctgcattt ctaatctata ttcatatata ctatcatatg
    32101 gttttacaac accatacaat ccacttatga ttcgaataca gctatttgca aactcttcat
    32161 ctttccttgt aaaattttct gcttctatat ttttatattg tattccatca taagttaaga
    32221 ttgcagggtt tcctaatcta tcaaatttaa tgctttcaaa tctattttta tttaatacag
    32281 ctaatttttc attaattttc attttatttt taaaatcttc tataccaaat gtctttatat
    32341 tttccactat ttcttttgtg ttatctataa aacaaggcaa actcaaatct ctatcaaata
    32401 cttctatatt cttcatattt ttagctggtg ataacattat aatcataaat tcttattact
    32461 cctgtttttc caaatattca agaatctctt gtgcatttgt gtctggtttt gctttttcaa
    32521 agactttttc aataattcca ttttcatcta taatatatgt cgcacgtaca actcccaatg
    32581 cagtttttcc atacattttt ttctctttcc atacatcaaa agcttttata gcaactaagt
    32641 cagggtcaga aagtaaaata aaaggaagtt catgcttttc tgcaaatttt tgatgtgatt
    32701 ttatactatc tttacttatc ccaataactt gtatatcttc ctttttaaat ccatcatatg
    32761 catttctaaa agcacaagct tgacgtgtac atcctggtgt attatcttta ggataaaaat
    32821 atactactac ttttttgcct ttaaaatctg acatacttac cttatttcca tctttatctt
    32881 ctagtgtaaa ttctggtgct tttgttccta tactcaacat aaatatctct ccttttgttt
    32941 ttattaat
//
LOCUS       DAGQWR010000028        32800 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.028, whole genome shotgun sequence.
ACCESSION   DAGQWR010000028 DAGQWR010000000
VERSION     DAGQWR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.028"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(2..694)
                     /locus_tag="MIW95_001134"
     CDS             complement(2..694)
                     /locus_tag="MIW95_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3150479.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWSSE
                     KKKGAPINKRGAPFSNKNSVGYGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHEITENSEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVANLNKDDIGDDEL
                     KISVDYGDRNDS"
     gene            complement(764..1273)
                     /locus_tag="MIW95_001135"
     CDS             complement(764..1273)
                     /locus_tag="MIW95_001135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150480.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            complement(1294..2268)
                     /locus_tag="MIW95_001136"
     CDS             complement(1294..2268)
                     /locus_tag="MIW95_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150481.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFEKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            complement(2463..3012)
                     /locus_tag="MIW95_001137"
                     /pseudo
     CDS             complement(2463..3012)
                     /locus_tag="MIW95_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            complement(3295..3774)
                     /locus_tag="MIW95_001138"
     CDS             complement(3295..3774)
                     /locus_tag="MIW95_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HBY3150482.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMVKELGVYLFGIDGLKLTV"
     gene            complement(3767..3904)
                     /locus_tag="MIW95_001139"
     CDS             complement(3767..3904)
                     /locus_tag="MIW95_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150483.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVEYCSRSCSKRARRSDLS
                     G"
     gene            complement(3928..5298)
                     /locus_tag="MIW95_001140"
     CDS             complement(3928..5298)
                     /locus_tag="MIW95_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY3150484.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCTLTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKNGAENA
                     IHNKIKDICISLKAEDYLNMPRKIDNKIEIHLDSKILKYYKELEKEKILELDRDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEAIENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            complement(5247..5573)
                     /locus_tag="MIW95_001141"
     CDS             complement(5247..5573)
                     /locus_tag="MIW95_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBY3150485.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            complement(5855..8272)
                     /locus_tag="MIW95_001142"
     CDS             complement(5855..8272)
                     /locus_tag="MIW95_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150486.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIENFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDTGVKKVNKSIFNGQLDNERNQIWAEAVELCKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITENWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            complement(8284..10242)
                     /locus_tag="MIW95_001143"
     CDS             complement(8284..10242)
                     /locus_tag="MIW95_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HBY3150487.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTAISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWFAGEKWRLDVFNSHGKIYEASASHMFKVPIESIKKGDPLRQKGKISELAL
                     GYGGSIGALTSMGAIKMGLDEEELQPLVTTWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VKLQHGIKFIYNPGVLFIELPSGRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            complement(10242..10814)
                     /locus_tag="MIW95_001144"
     CDS             complement(10242..10814)
                     /locus_tag="MIW95_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HBY3150488.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKMTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            complement(10837..11409)
                     /locus_tag="MIW95_001145"
     CDS             complement(10837..11409)
                     /locus_tag="MIW95_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HBY3150489.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            complement(11462..11797)
                     /locus_tag="MIW95_001146"
     CDS             complement(11462..11797)
                     /locus_tag="MIW95_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150490.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     RLNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            complement(11812..12978)
                     /locus_tag="MIW95_001147"
     CDS             complement(11812..12978)
                     /locus_tag="MIW95_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HBY3150491.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPSTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     AFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQNYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            complement(12990..13427)
                     /locus_tag="MIW95_001148"
     CDS             complement(12990..13427)
                     /locus_tag="MIW95_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150492.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            complement(13498..13647)
                     /locus_tag="MIW95_001149"
     CDS             complement(13498..13647)
                     /locus_tag="MIW95_001149"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150493.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            complement(13628..14020)
                     /locus_tag="MIW95_001150"
     CDS             complement(13628..14020)
                     /locus_tag="MIW95_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3150494.1"
                     /translation="MDNTNEIVKRFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVKRGDEIAES"
     gene            complement(14182..14364)
                     /locus_tag="MIW95_001151"
     CDS             complement(14182..14364)
                     /locus_tag="MIW95_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150495.1"
                     /translation="MKHLMDALDANMIICVGGVQLPRGKSTLVDLLNENGYRAIKDCD
                     VFKIELNKPIQKTPQK"
     gene            complement(14500..15177)
                     /locus_tag="MIW95_001152"
     CDS             complement(14500..15177)
                     /locus_tag="MIW95_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150496.1"
                     /translation="MEFLWKKLINIINKIKQYRRNKKMKENNRIVSIGVDGEIDLTDK
                     ELEMVNNFKKVMNSINNKIGIDCGISLSDKDIEYNKNILDKIASKVAWHYKKMNFEHL
                     EMDEKFFDDFTFKFMLKFFGDIEEDELIKYLTYIKEESNELSARERIFYKIVDTLDTI
                     ADIKIADQNKIEKEVGMCMGEDNYITYCIDEDLIKFYIKDEEELVIDKNSPLLYMLNT
                     LFYEVHE"
     gene            complement(15149..15952)
                     /locus_tag="MIW95_001153"
     CDS             complement(15149..15952)
                     /locus_tag="MIW95_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBY3150497.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETEIRNISNNFNNNKERFIE
                     GKHYFCLQGDDLRAFKRDSYDIRIAPNVNKLYLWTERGANRHCKILDTDKAWEQFDNL
                     EESYFNKKKDTLITNQLSPELQLFKQIFDTVAKQEIEQKQIKQDITETKQEIKSIKDV
                     VTLNTTDWREETRKLIVSMAQNLGGNEYINTLRKESYELLCKRFNVDLKRRLNNRRSK
                     MAEKGVCKSRREKFNYLDVIQEQNYLIEGYVIVIKDMAIKYGVFMEKTN"
     gene            complement(15945..16124)
                     /locus_tag="MIW95_001154"
     CDS             complement(15945..16124)
                     /locus_tag="MIW95_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150498.1"
                     /translation="MNSNKKRITVRMPEKLNEEITKKSKYLGLTKNSFILDILWKEFE
                     LLEYRSYKKEVDKHE"
     gene            16260..16439
                     /locus_tag="MIW95_001155"
     CDS             16260..16439
                     /locus_tag="MIW95_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBY3150499.1"
                     /translation="MSTSLPKYTLRINRVLLEKIKYIAESEGRSANKEIEQIIKKHIE
                     DYEQRKGEIKINIEE"
     gene            complement(16618..17427)
                     /locus_tag="MIW95_001156"
     CDS             complement(16618..17427)
                     /locus_tag="MIW95_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBY3150500.1"
                     /translation="MNNLQIFKNKTFGEIRVIELNGEFWFVGKDIAEQLGYKDTSDAL
                     KRHVDDEDKGVGEIPTPGGNQNMKVINESGLYSLILSSKLPSAKLFKRWVTNEILPSI
                     RSTGTYNMIDLQTKLPTTYKEALQHLIEQVEVNEQLQLESKMKDQVIKELKPKADYTD
                     MILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHSET
                     IDITRSNGMTDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKEYSYQISMLG"
     gene            complement(17475..17678)
                     /locus_tag="MIW95_001157"
     CDS             complement(17475..17678)
                     /locus_tag="MIW95_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3150501.1"
                     /translation="MKKRSLKAGRIEAGYTQEELAYKIGIAKSTYNLKENGKRNFTEK
                     EMIMISYILNKTMDELFLEKRLT"
     gene            17855..18196
                     /locus_tag="MIW95_001158"
     CDS             17855..18196
                     /locus_tag="MIW95_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBY3150502.1"
                     /translation="MATFGERFKQLREEKKLTQDELVSKFNKVYFTSFNKSTISQYEN
                     NKRKPEINILENWADFFDVSIDYLLGRTLVRNHIDTVAAHRVNPHKDLPEEAQEQLND
                     YIEFLMNKYKK"
     gene            18208..18756
                     /locus_tag="MIW95_001159"
     CDS             18208..18756
                     /locus_tag="MIW95_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3150503.1"
                     /translation="MLILKKLRTEKGISLDKLSTDLNINKSTLSRIENGLREPKESFI
                     KDCSDYFGVSTDYLIGKINIDDSNKLTKCLNSTFPIRLKELRKKKELTQAELSKLLNC
                     SLSKIAMLETSKREPVKEDLLRISEFFNVSVDYLLGKTSIENYITTDEISKIIKSYES
                     LPKEAQEHINSYIEFLVDRYKK"
     gene            18832..19338
                     /locus_tag="MIW95_001160"
     CDS             18832..19338
                     /locus_tag="MIW95_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBY3150504.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            19958..20827
                     /locus_tag="MIW95_001161"
     CDS             19958..20827
                     /locus_tag="MIW95_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HBY3150505.1"
                     /translation="MIKNFDEWKEYDGFSYGSGRSEKIWLINEKTKDIGLFKFPKTKE
                     TGEYWAEKLACEIASLLKIPCANVEIGTYNKRIGSMSYLINKNDEKLIEGVWLINKYY
                     PCYNTDKLYDYKNNEYYSLKMIFKSIEEYNIKNDFLKMLIFDYLIGNRDRHQNNWALL
                     ESQNKITLSPLYDNGSSLCCYVNIENIDSFFTDHQRFEALINTRSRSRIRIDGKNKKE
                     PTHLEVLKYLNSNYYEQTIDFVEKIINLMNEVNINTLIDNYSENIISPKLKKLLKIYL
                     KEKVLRIKNIYNL"
     gene            20840..21547
                     /locus_tag="MIW95_001162"
     CDS             20840..21547
                     /locus_tag="MIW95_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBY3150506.1"
                     /translation="MSIKNGKDFLYVIWKDPKSRRQFIIGKLAKNGLYEFSYEYEIDE
                     AIKAGFNALISFDDISKTYYSEDLFSPFASRLPDKKRKDINKILDKYDMSEYDTYELL
                     KRSGARLPIDTLEFVDPIPLDIDKNIIRYFYIAGVRHYAGCEGHECDNSIELESNEAL
                     VLELEPENKFDPHAIKVFDKKENHIGYIPRYYSGELTSIINRGLKYNCIIKFVNKENN
                     CDECIKVKLEILFDEYN"
     gene            21626..22756
                     /locus_tag="MIW95_001163"
     CDS             21626..22756
                     /locus_tag="MIW95_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBY3150507.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(23114..24802)
                     /gene="ilvB"
                     /locus_tag="MIW95_001164"
     CDS             complement(23114..24802)
                     /gene="ilvB"
                     /locus_tag="MIW95_001164"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBY3150508.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWSAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKAGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSNMEELGKVLDVIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(24855..25850)
                     /gene="ilvC"
                     /locus_tag="MIW95_001165"
     CDS             complement(24855..25850)
                     /gene="ilvC"
                     /locus_tag="MIW95_001165"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBY3150509.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            complement(26120..26359)
                     /locus_tag="MIW95_001166"
     CDS             complement(26120..26359)
                     /locus_tag="MIW95_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBY3150510.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     misc_binding    complement(26472..26729)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(26890..27651)
                     /locus_tag="MIW95_001167"
     CDS             complement(26890..27651)
                     /locus_tag="MIW95_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBY3150511.1"
                     /translation="MNTGSKDIDTLNVNFRGIEIKAVLVYKNRKNISIKIDPFGKIIV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLKDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERTVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     gene            complement(27656..28477)
                     /locus_tag="MIW95_001168"
     CDS             complement(27656..28477)
                     /locus_tag="MIW95_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HBY3150512.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANYER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKRETGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            complement(28519..29274)
                     /locus_tag="MIW95_001169"
     CDS             complement(28519..29274)
                     /locus_tag="MIW95_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBY3150513.1"
                     /translation="MTTYKKLKKSIFKISIFSICLLVSLVDINPIFAGSSSNETSNSD
                     TIKVLNAKSLNFKQGNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEDSSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDFPVKDFESINKYQDFYFNKN
                     GNLVISFEEYEVVPGYMGAVEFVIPNKVIKRLR"
     gene            complement(29317..30186)
                     /locus_tag="MIW95_001170"
     CDS             complement(29317..30186)
                     /locus_tag="MIW95_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBY3150514.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGVIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKAHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            complement(30198..30680)
                     /locus_tag="MIW95_001171"
     CDS             complement(30198..30680)
                     /locus_tag="MIW95_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY3150515.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            complement(30732..31664)
                     /locus_tag="MIW95_001172"
     CDS             complement(30732..31664)
                     /locus_tag="MIW95_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBY3150516.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKEYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            complement(31757..32590)
                     /locus_tag="MIW95_001173"
     CDS             complement(31757..32590)
                     /locus_tag="MIW95_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBY3150517.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
BASE COUNT        10377 a         5751 c         3370 g        13302 t
ORIGIN      
        1 tctaactatc atttctatca ccataatcta cacttatttt caactcatca tctccaatat
       61 catctttatt taggttagca acctcacatt tcaacttctc aactcttgtt ttctgctcct
      121 ctgtagccaa attccaatcc ttatgaatca tttcatcata ctgcttaatt aaactcctta
      181 actcactcat agctctactc tgtgcattaa gaaaagatgc ttgcctatcc catgcaaatt
      241 gaaattcata ctctatcttc tcactatttt ctgtgatttc atgtttcttt aattccttaa
      301 tcatttcctc tttatcttta acatacatta tcttctgtgc tcttattatt gctgcgtatt
      361 gaattgttat ctgttcccaa agaatatcaa atttatcttt tatagatatt tcttgtatta
      421 attccctagt ttcttcaggt aagtattttg agaagaaacc aaacttttca gcatttttat
      481 tctcttttgg agcaccataa ccaactgaat ttttattaga aaagggtgca ccccttttat
      541 ttataggtgc acccttcttt ttttcactag accaattgta tctttttatc catgacttta
      601 aagtatttaa actaatgtca tactttgctg atatttcctt ttgtttcatt ccttttaagt
      661 aatcttgttt taccttttct ttgacatctt gcacatcacc acctctttat ttgtttgttt
      721 tgggaataaa aaaacaatcc tatttaatag gattgtttca aatctagtct cctctatcct
      781 tccaggataa atgaaacata cttattatta atgtagttag agataaaatt atatgaactg
      841 taaaaaatat taaaacatag aaaattatat tagaataata tcgagtaagc attattgtat
      901 tgataaaaca tattacaaca accaaaatct ccattattac agtataatat gcttttaact
      961 gtatgaattg aaagtatgat atattaaatc catttacctt tctttctgtt tttatatctt
     1021 ttgcttctga tattgatttg ttatatgtag taaacaataa tgtaataatg caaactccaa
     1081 aagctactaa tagtcctgac aaagttagta catctttgtt taaagaaatt acaacttctt
     1141 tattaattga cgaattaaaa aacatgtatg aaataataca tattaatact ggtgttaaaa
     1201 ctaactctac tacttcttta gaatttatag ccttgtaata attgagtaag atatttaata
     1261 cctttttata cattttaaca cctccttagc aatctatgct tcactcatag caacttcttg
     1321 atacattaaa ttaaaaaaaa ctgaattatt ctcaattaaa tcatctatca aattattcat
     1381 cttctcaaaa atgtcttcac tatctatatg attatctata tcaagagaaa cctctatttc
     1441 tctacacaac tttataccct cagtatccaa ctttaatttt ctcttatttt tctctccctc
     1501 aattattatc ctatttattt ttttatttct aaattcctct cctttaatat atttttcgca
     1561 acatctttta acttcactag gtaaatatct agttctaaat gtgggcttat atatcaaatc
     1621 gttatgtcgt cttgaattat ttaaattaga aaacaaatta tcttcatcag ttccaaactt
     1681 ctctctatca acatttaaag ttatttttga aacaccatct aaatctaata tttgttctat
     1741 aaaatcttta cttgctattt gtgtaatatt aattgatgtt tttgacatat tattatcaaa
     1801 tttattattt tcaatcatat cctcataata tagatgtaag tttttactta gttcaatcat
     1861 gggaaccccc acaaaaactc tttcaaataa tactactgct atattatttt ccttcataaa
     1921 tttaataaat aggtgctgct tattgctatc tccttgatcc atttttttat taccctctac
     1981 ttctttagta tgagcattta taatatttgt attctgattg tatttagtat attcaagttt
     2041 caccttgatt attaaatcat cctcgctaca ttttacttct gtaatccaaa actttttatc
     2101 tttagtaata ttaattccat ttttgttttc tttacaatat tctttatcct taaagaaggt
     2161 ctttattacc tcttttaaaa tttcttcatc tgaatagaca ctcccattaa ttttatttat
     2221 aatatctatt ctataaacat caatatatct ttttttaaaa ttactcatta taccattatt
     2281 cctttcacaa atatttataa tgatataatt ctatataatc agtcaatatc cttcaataat
     2341 cattcgacaa ccacaaaata agtctaaata atacttttac cttattcctg tctataatgt
     2401 acaaatatat ttcattttta attcattaca taaaaaaaaa gaccctctat taatacagtc
     2461 atttaaatca tttcgattaa ttctttaatc tttttatata cctctttata attcatacct
     2521 ttatctatca acctaggtaa tttcatagat ataactcttt caagtacttg tattaataaa
     2581 aaaactcttg tatttcatct gtaaaatctt ttctgacctt ttaccttctg ttcttatatc
     2641 cagccattct tgatcttttt ctgtggcaat atagtaacca tgtattctaa tttgtttaag
     2701 tgcttttgta acccacttcg taaatagttt tgcttctgct ttattacttc taaatgacat
     2761 attgtacaca gcttcttctg taacaaaagt agtaccaaag ttaggcaatt tatctttaaa
     2821 gtttctagtg taggaatctc cgacagtaga ctcattaaat ttctttttat attctctatc
     2881 tatatttctt aatgtatcac gaatatttac tatgcctagt tcctctccta cgtcatttgc
     2941 attaaaccaa acttcttctc cattttttga ccatatcatc cttacatttt tctcttgtaa
     3001 aattttcaac atactactac ctcctaaatt agttattaat aaagaatatt caatttgaac
     3061 acccttattt tcgacccttc aatatgagag attaaaattg tataaaaaaa gacctagaaa
     3121 tcactctaag tcatgaatta ctcatttatc attctcctta tttacaaaat aaaaaagact
     3181 aagttggggt aacttagcct ttttcaaggg ggaatatatt atacacttgt ttcatactac
     3241 cattataaca ggggttaaat atcattaaaa tatcatcttt ttatcatgta tttatcaaac
     3301 agttaatttc aagccatcta taccaaataa atacactcca agttctttta ccatttcatt
     3361 aatccaacgt cttgatgtaa ctactccaca atttaatatc tctgctattt cttcatatgt
     3421 tttttcatca caatagtaaa gctctaaagc tctatacttt tcataagaat gtaaacgctc
     3481 ttgtcttaat ttcaaagttt tcattgctga atctatatgt gctatcataa taatagttct
     3541 tgctttactt ttctttatac ttaaaatata caactcttca ttatcaagct tacctaaatc
     3601 atcctctaag taatctacat ctttagcttc acttaccgct gagtctatat gtctcttaaa
     3661 atcattataa tgcttcatca ataatctagt attatataaa acgccttttt tcctatcttc
     3721 tctttcttct tttcttaact ctcttacaat ttcttttata ctttctttat ccacttaaat
     3781 cactccttct tgctctcttc gaacaacttc tactgcaata ctcaacttta gatttatcca
     3841 acttataaaa tcttctacca caccaagcac atcttactat tttacctggg cttataagtt
     3901 ccatcttttc tttttacttt cataccttca ctccctatat tcttttaatt ttgcttttac
     3961 tgcttcaagt aatgcacttt gccctttatc cttattctct atagcttcca ttacttgttc
     4021 atctattgta cctttacaga ttagatgatg gataataaca gtttcccttt gaccctgtct
     4081 ataaagcctt gcattagctt gttgatacag ttctaagctc caagttaatc caaaccaaac
     4141 aattatactt ccacctgatt gtaaattaag tccatgtcct gtacttgctg gatgacaaag
     4201 taataattgt attcttccat tattccaatc atatatatct tttgagtttt ctattgttct
     4261 tggtttcaaa ctcttaaact ctttcattaa acgattataa tcatgtttat agttataaaa
     4321 aactataata ggcttaccat ttgaaacatc tataatttct tttaaagctt ctaatttttc
     4381 tctatggagt tcctttacat ttttatcatt atcataaatt gctccattag ctaattgtaa
     4441 aagtttattt gctgctacag ctgctgatga agctgttatt atatctctat ctaattctaa
     4501 tatcttctct ttttctaatt ctttgtaata tttaagtatt ttactatcaa ggtgtatttc
     4561 aattttatta tctatttttc taggcatatt caaataatct tctgctttta agcttataca
     4621 aatatctttt attttattat gtattgcatt ttctgctcca tttttaggtt gccaattata
     4681 tatagctcct gtttgataat ttttttgtcc tggctcaaag tatcgttctt tatatcctgt
     4741 aatagtttta cctagtcttt cacctctatc aagtaagtac atctgtgccc ataagtctat
     4801 taaactattt ggtgttggtg taccagtaag cccaactact cttttagtta aaggcaatac
     4861 tttctttaaa cttttaaatc gttgtgcttt atttgattta aaagaactaa gttcatctat
     4921 cacaaccata tcaaatggcc attttctttt ataaaaatcc actatccagg gtaccatttc
     4981 tctatttatt atataaatat ctgagtctgt acttaaagct ctcactctat ctaatttact
     5041 accaagaact ttagatactt ttagatgctt tagatgttcc cattttttta cttcactact
     5101 ccaggtatct cttgcaactc ttaaaggtgc tataactaaa acttttgata tatcaaagta
     5161 atcatacatt aattcactta tagctgttag agtacagact gttttaccta atcccatatc
     5221 taatagtaaa cctatattat tattatctat agtttttcta attgtgtatt cttggtatgg
     5281 atgtggttta aattccatcc cttaacctcc ttgataaaat catctatctc ttttaatgtg
     5341 cttacacact taactttaaa tcctaattct cttaattctc gcattttata ttgttgaagc
     5401 ttccttagtt ttttacctgg tgctttaagt tctacaaata taacatgtcc ttctggtaat
     5461 aagacaatcc tatctggcac acctgcctca cctggtgaaa taaacttcat agcctttcca
     5521 cctaacaact caatctcttt tttaagtctt ttttctattt ttgattctaa caaaatatca
     5581 cttccttaaa atttaagtgt caacaatgtc aacagatttt ctatatatat gtatatatac
     5641 atattaggca tacatatgta tatacgtata tgcctaatat tacttatact actttatact
     5701 taatttttgt tgacattgtt gacattaata cctcaaacat tgtaattact gcatcttagg
     5761 tgtcaacata agcgtcaaca aagccatttt ttttgttgac attgttgaca cttttaaaat
     5821 tttcaaattt actctgtttt taattctgtt gacactaact tttacgtaca taagctctct
     5881 gcctaccata tattttccca aaccttaaat ttccattata tgatatccaa tcatctaatc
     5941 cttttaatat atcattaatt tctcttgata agataggcgt gagttgttta ggttctccat
     6001 taaatagctc aacccatatc tccataacac acgttttttc tcttaatatt gttccttctt
     6061 ttaaatcacc aaaatctgaa ccatgaatat attctctctt ttctgaaata cttaaatcat
     6121 accaattctc agtaataggt ttatttagat actcttcgat aattccagat ttagcatttt
     6181 cttctgagtg agttctttgt tgtctttcag cttctttttt ctcctcatct gatagatata
     6241 gctgttcatt ggctttacat aattctactg cttctgccca aatttgattt ctttcattat
     6301 caagttgacc attaaatata ctcttgttaa ccttctttac tcctgtatct attggccaaa
     6361 accttctatt cccggtctta tctcttaaaa attctttatc attagttgtt cctataacta
     6421 cacattgacg taaaaacctt gatgttctct tcccatatgc gaccctatat atatcctctg
     6481 atttgcttag aaaatgctta actgcctcaa tatctgcttt tttagtagcc atcatttcac
     6541 ccatttccag caaccatact ccttgtaatt gttcatacgc ttctttacct tgtacagtag
     6601 ttaaactatc agaataccag tctccaccaa gttttttaat aaaagtactt tttcccattc
     6661 cttgaggacc agataaaacc atcatattat caaactttat tcctggatta aatactcttg
     6721 ctactgctgc tactaaaact tttcttatta tagtccttgt atagtggtta tcttctgcac
     6781 ctaaatagtc aattaaaagt gtatccactc tcttaatgcc atcccacttt aaagaattta
     6841 aataatcttt tataggatga aaagtattat tttcaaaagc aattattaga gcatcattta
     6901 cttttgatgg tgatgagata ttgtaaattg tttctatatg atgtctaagc ccagaatcat
     6961 cactatcatt ccaatcattt aatttatcat cttttctcca aggtaactta cctaaaacga
     7021 cagctctatt tgaaaattca ttataagcta tttttccttt caaatatggg tcattttcta
     7081 taaacattaa gatattgttt gttgttttct tataacttcc cttattgtca taatctaacc
     7141 tagttaacca ttcatcatct tcaaaatcta tatcaccaaa atcatcctta gctttatcaa
     7201 ggttttctct tcctatagtc tttcgtactt tagtgtcact gcttgcaaat tcgctcattc
     7261 gagtaaatga aggtaatcta tttacaggcg tttcgggttt agcatcttca tcaagttcac
     7321 caaatttatg tatcctaact aaatcaaaag cattgcataa aattccgctt gctgggtctg
     7381 taccatgatg actatatgaa aacttgtcat cataaataac tactccacca cttgtactac
     7441 cttctgaata tgtatacctg gtttcatcaa taccaggaat atatacttca tttaagaaat
     7501 tttctatagt ttcttttata ctataggacc tgcaaaatgc acctataatt ccagcctttt
     7561 caatagggtc ttgttgtttt tttaattgtg tattaaactt ttgcctctcc ctcgaacttt
     7621 ctggccaata gcttacatct gtccagtcta aatataaatc tagtatttca tttgggttta
     7681 aaaactctcc atcttgaaat ttaaatatat agtctccatc aattgaagta cttggaaaat
     7741 acataagtct atgaggttga tatgtagtat catcaaacat atctattcct atagtatctg
     7801 ctatcatcct ggaaattgct tgatattctt ctggtagtac tggtctagtt aaaggtatta
     7861 caagtctata tctgggatta ttctcagtat gtgagtgcgt agaatacata agacaagcat
     7921 aatcatttaa taatgttata tcctcccata tatctttatt cgcataatct atatctaaag
     7981 ttataatact tctatttgca atattctctg cttttcgtct tccatttttt aagcttccac
     8041 ctacaaatcc accaacatct ttaactctat ctttttcagt ctttgacatc ttcctatatt
     8101 ctgtatatgt ttcttgagtt cttaatgttt tacttaatct atttacaagt tcggaccata
     8161 aaatactttt atttttccaa tgtgtttcta atttattttt tcctatggcc agcatgagtt
     8221 ggccatcatg tcttacattt atgtgttcaa tttcactggc ctttatatcc ataaatcaat
     8281 cacctagtct ttcttataat aatcacattc atatccatct gctttaagag gaagaccttt
     8341 agcccaagat atttcttttc ccataatact gttaacttct tctaaagaac ctcctttttt
     8401 atctacatca attacaagct catcatgtac atgcattaca atgctataac ctgcatctgt
     8461 cacattaaac atagcctctc ttaagcaatc tctagctgta gcttgaacaa tattctcaac
     8521 taacttaggt ccataagtat ctattctttt ccattgttta cttgtctgtt ccataccttc
     8581 atatgttatc ttatccccac taaatgtagt atgaggctct atcttaggtc ttagatatga
     8641 taatcttcta ccacttggta gttctataaa taaaactcct ggattataaa taaatttaat
     8701 cccatgttgg agttttacta tagtcctatc ttttatggct tttttagctg ccttatctac
     8761 atcccaccaa aatttagtta tatttggatt agcatttctc catgttgtta caagaggttg
     8821 aagctcctct tcatcaagac ccatttttat agcccccatt gatgttaaag ctcctatgct
     8881 tcccccatat ccaagagcta gctctgaaat cttacctttt tgtctaagtg ggtcaccttt
     8941 tttaatactt tctattggaa ctttaaacat atgactagca ctagcttcat atattttccc
     9001 gtgagaatta aatacatcta atctccactt ctcaccagcg aaccaagcta taaccctagc
     9061 ttctattgca ctaaaatctg atactataaa tctatgacct tcacttggta taaaagctgt
     9121 ccttatcaat tgacttaaga catctggtac actatcatat aaaagctcta ttaaatcaaa
     9181 atctccttct tttaataggt ttctagctag gtctaaatcc tctatatgat tttgtggtaa
     9241 attctgtact tgtactaatc ttcctgccca tctaccagtc ctattagccc cataaaactg
     9301 tagtagacct cttactctat tgtcattacc tttagctaat ttcatagcct catatttctt
     9361 tatagaagtt ttggacatta attttctaag ttctaaaatt ctaactacat tttcatcatc
     9421 aacttgtttt aatatttctg gaatactttc ttttgttagg cttgtaatct caaagccaac
     9481 tttatcactt aaccattttt ttaattgagc tggactattt ggattattta gaccagttat
     9541 tttaattgct tcttttgtaa gtttttcagt atatctttta tcacattcta ttgcattctc
     9601 tattaactct gtatcaactt taataccagt atcattaatt ctctggtcta aataccataa
     9661 tttaatttct ctttcggtag tcttatactt actaagtttg tttcttattt ctctttcaac
     9721 tacaacatct tgtttacaat attctttaaa tttattccat ttttccatat catgtattgg
     9781 taaatttcta gttcttccct tgttaacttt tgtagcctta caaggtttac agaaatattg
     9841 tattaatgct ttaccttctt tcattttctg tttatcttca ttaaacttta aagccttaga
     9901 cacactatct aaacttcctg gaagtcctag tgttaacgcc tttatcattg tacatgacca
     9961 ctcatttggt tttaaattaa tatttaaaaa tttacttatt gctgttcttt caaaattagc
    10021 attaaatgct gattttataa ctttattatc atttaaagct tctattactt cttttggtaa
    10081 ctcttcatca tttactaaat caataacttt tacttcttca ttatcaaaag cataggcaaa
    10141 taacagtatc tcaaaattag cagagtctac atatctgtag actccaactt tttttatatc
    10201 taaatcacta tatgtttcta tatcaattga taaggtcctc attaacttaa gaagtcctct
    10261 tcttcatctt catattcaaa gtcatctgag aaatctgctt ctgcactagc tctagctcct
    10321 ccaagtactt ctccatctgc taacttttga acattttgta agccacaacc tattccttta
    10381 tttcctgcac tattatatgg gaaaaagttt atactaactc ttccataaca accactatat
    10441 acctctgtat tatctaatat ttcatttaag tctttatcaa ctattcctgg cttttgagta
    10501 ctatttgcat ttaaaaaata cattcctaca tattcctctg cttcatcagc tctttctgca
    10561 tctccatcac gaagaggtgt ttttaaatta cctggtaatt ttccacccca tttagaagtt
    10621 ttaccttgtt ctttagctgc atcaatagcc ttctttattc ttcctaaagt catcttatct
    10681 gattttggta ttaaaataca aactgaatac ttgggctctg caccttctac cattgctctg
    10741 cttttaaaga tattgcaata acttaatctt acctttcctg ttactacctt tgttgattgt
    10801 actgaattac tcataatttt cttcctccta atatttttat atttggtata ttgattttat
    10861 atttgaagtt atttctccaa atttatctaa atctctaact tgcttactaa acaatttaat
    10921 tcttccacaa ataactgttg aagaaacatt taataactct cctatatcag atatcttttt
    10981 atcttgtaaa atccaatcta ctattttatc aatatctttt aaatatgact ttcttgcaat
    11041 ttttcttata taatcaacat catagttaaa ttgaatttca taaaatccat gtatatcttt
    11101 taatggtctt ttactatctt caaatacact tacatctaaa ccttctatag agtttctata
    11161 atgtttttcg ccataacgca aagctttaaa catttctatt cttatgtaag aaacagccac
    11221 tgtagaaaac ttgcctctgt tactatcata atttaatgct gctttatata acccaataca
    11281 tccttcttga taaaactctt catataagta tggatgttgc tctacataag acttaaaaaa
    11341 tctatttata ctaaaatgta caagattcaa attattttca actagtttag ttatttcttg
    11401 attactcata aaattatatc cccttttaaa tctttttata gctactagga attaaatatt
    11461 tttaattatc ctttgctctt actgatacaa agcatactgg actaactttt atatgttctg
    11521 gtatatacac tctatcaaga tgattgtcat ttacttcgag gaaagttgac atcactttat
    11581 cttttattaa actttctctt ttaaaatcca tcactataac ttttgcattt tcttcagttg
    11641 agttatattc tgtaatattt agtctcttta acatatctag taaattatct ttttcataat
    11701 ctaaatcttt ttcgatactc ttttttctat cctttaaatc taaaattata cttatgcttt
    11761 catctaattt atcttttata cctgcatcta atggcatgta atatccccca tttatatttc
    11821 aaaatctttt ttcgctgaat ctatactatt aatttctgac cttttatcac tttctactac
    11881 taaagtagct tttccaactg gttttataat taaatcactt agtagtttag caaaactctt
    11941 tttacctata gctttctcca tatcactaat tccttttaag gtccttggct tataaatttt
    12001 ttcttcatca taatctgaat ttaataaaac cttagcaact tcttgctcat ctacatactt
    12061 tctattactt ctgccttcta caagcttata tcctggatat ttaacaccat gcttttcagc
    12121 ttgttctagt gcataatttt gaacatcctt taaccaatct tgtatattct tagcaaaacc
    12181 taaaatatct gctatctcat atttattaag agcaaatgtg tcagcaaaat catattttct
    12241 agctagttta aggttatcct cagctctttt tctgcaatca tttttagctc tacaaaatcc
    12301 acaatgacta ccacttacaa acgctccttc accattaaaa gccatttgag ccttcttctt
    12361 aacattatct gcccatgtaa gtaatttagt aacttctatt tcttcacttg atatgttatc
    12421 aagtctaggt tgaattattg ttgttttaat taaatcaata tcgtatagca tttcaaactg
    12481 attatatgcc cctaaaccat atagtctaag ttgaggattc tctatagctg aaacttctaa
    12541 accttttcca tactttaggt ctataacttg aagtatacca tcagatatta caacaacgtc
    12601 tcctgttcca aatccttctg gaacccactc actaaaatcg agtctttctt ctaacatcac
    12661 tattacatca tcacatatag ctttactatc atttactaat tcaactacat tctcaacata
    12721 agattgtata tagtcctcca tttcagagtt ataatactca cttttctgta tctttttaat
    12781 tcttgcatta tatgcctttt tacttatttt ttcatactct agcattaatt taacttctgc
    12841 taattcatgt gcaactgttc cttcttctgc atatatacta gttgatggtg gatagttttc
    12901 ttctaatttt atactaggag tgcagtgaag ccatctatgg gctccacttg cactaagtct
    12961 tgcatgttgt aatggcattt atgtaccccc tataaacttt ctaatttgtt cataaaagct
    13021 gaataatctt cttcttttac ttcacttaac ttactagctc caaactcacc aaataactcc
    13081 ttaagcttat ccttcttacc tgccttactt acttgtgctg ccttagttct tacctcttct
    13141 tttgtgtatt taacttcact agttgtattt tcattattct tttctatttc ttcctttgct
    13201 tctgctattt cttctttaac gtcttctttt acttctacct ttttaatctt ttcagctttt
    13261 ttaacttcaa ctttcttctc ttctttcata tctgttatat ttataggttc tatctgcatt
    13321 gcttttccta gattaagacc tcctaaagca ttcgccacta ctaatagtgc atttgtaaat
    13381 tctggtgctt ctactttaac ttttacattt acattcactt caaccatttt aaatctctcc
    13441 tttttgtgtt atactttact tgtgttatat tttatttttt atttttttgt gtgttggtta
    13501 ttttaccaac acttttttat ttaatattcc aaccgatatt ttcttaccag ttttaatatc
    13561 tttaaatact atatctgcta taacttttcc atctttttta agagttacta cattcttatt
    13621 attagtatca agactcagca atttcatccc ccctctttac tgcttttaaa agttcatcca
    13681 aatttttacc ttgatttctt tcaataaaat catcaacttc atatcttgaa atttttctac
    13741 catcacctct aactagtgat tttatcaaac ctgtgcttac taacctacgc ataaaagctg
    13801 tatctaactt taaaattccc cttgcttctt ctactgttat tagataattt ggataacctc
    13861 tttttattaa aacaactata tctttaggct ccagtacctt taccttttgt tcaatagttt
    13921 gactcttaac tctatctgtc tcttgtttac ttatctctat ttctattaag ctttttaagc
    13981 tatcactaaa tcttttaact atttcatttg tgttatccat tttttaatcc cctcattatt
    14041 atcaaaatat tctgtttttt atttcccaac taattcatct aaggtaacat ctaaatagcc
    14101 agctattttt attaatgtgc atatagttgg atttttaata tatctaaaag ttccaaattt
    14161 ttctctataa agtagatact tttatttttg tggtgttttt tgtatgggtt tattaagctc
    14221 tatcttaaaa acatcacaat cttttatagc tctataaccg ttttcattca ataaatctac
    14281 taatgttgac ttccctcttg gtaattgtac acccccaaca catataatca tatttgcatc
    14341 aagcgcatcc attaaatgtt ttatctctct tttacttaat aaaggtttta agaattcttt
    14401 tacttctttt ttttctttta ctcatgtttt gtcctcccta ttttctctaa tttaaaatta
    14461 aattaattgt atttaatctt tactagtaaa atacttttat tattcatgta cttcataaaa
    14521 tagtgtgttt aacatatata gcaaaggact atttttatct ataactaatt cttcttcatc
    14581 tttaatgtaa aattttatta agtcttcatc tatacaataa gttatataat tatcttcgcc
    14641 catgcacata cctacttctt tttctatctt attttggtct gctattttta tatctgctat
    14701 tgtatcaaga gtatctacta ttttataaaa tatcctttct ctagcactca attcgttact
    14761 ttcttctttt atataagtaa gatattttat taattcatct tcttctatat caccaaagaa
    14821 ttttaacata aacttaaaag taaaatcatc aaagaatttt tcatccatct ctaagtgttc
    14881 aaaattcatt ttcttataat gccatgctac ttttgatgca atcttatcca atatgttttt
    14941 attatattct atatctttat cactaagtga aattccacaa tcaataccta ttttattgtt
    15001 aatgctattc ataactttct taaaattatt taccatttct aattccttat ctgttaagtc
    15061 tatctctcca tcaactccaa tactcactat tctattattt tctttcattt tcttattcct
    15121 cctatattgt ttaattttat taataatatt aattagtttt ttccataaaa actccatatt
    15181 taatagccat atcttttatt acaattacat aaccttctat taaataattt tgttcttgta
    15241 taacatccag gtaattaaat ttttcccttc tagacttgca tacacctttt tctgccattt
    15301 tacttcttct attatttaat cttcgcttca agtcaacatt aaatctttta caaagaagtt
    15361 cataactttc ttttctaaga gtatttatat attcatttcc acctaaattt tgtgccattg
    15421 atactatcaa cttgcgtgtt tcctctctcc aatcagttgt attcaatgta acaacatctt
    15481 ttatgctttt tatttcttgt ttagtttctg ttatatcttg ttttatttgt ttttgctcta
    15541 tttcttgttt tgctacagta tcaaatattt gcttaaacag ttgtagttct ggacttaatt
    15601 gatttgtaat taaagtatct ttctttttgt taaagtaaga ctcttctaag ttatcaaact
    15661 gctcccaagc tttgtcagta tccaatattt tgcagtgtct gtttgctcct ctttcagtcc
    15721 aaagataaag tttgtttaca ttaggtgcaa ttcttatatc atatgaatcg cgtttaaatg
    15781 cccttaaatc atcaccttgc agacaaaaat aatgtttacc ttcaataaat ctctctttat
    15841 tattattgaa attgttactt atatttctaa tttcagtttc atatacatct gctaattgtt
    15901 gtgtagttag aactctctca ttatttcttt ctattacttg taagttattc atgtttatct
    15961 acctcctttt tataacttct atattctaat aactcaaatt ccttccatag tatatctaat
    16021 ataaatgagt tcttagttaa ccctaagtat tttgattttt tcgttatttc ttcatttaat
    16081 ttttctggca tcctaacagt tattctcttt ttatttgagt tcattttgcc gtcaacccct
    16141 ttcttgtttt aataatatca cgccgtcttt atgccgtcaa tatattttat agattttcta
    16201 taaattattt ttatgatata attatgacat catttagact ttaaaaagga ggtattatta
    16261 tgtcaacttc actacctaaa tacactttaa gaataaacag agttctgctt gaaaaaatta
    16321 aatatatagc tgaaagcgaa ggtcgttctg ctaataagga aattgaacaa ataataaaaa
    16381 aacatattga agattatgag caaagaaaag gagaaattaa aattaatatt gaagaataat
    16441 ttatatactc ttgctaatca ctgataatat gtactcgttt actgacatac ctctttgatt
    16501 agcttcttct ttacatttat tgtataattc tttcgtaata ctcaaggtat acttctttct
    16561 attactcaac ctttaaatca cctctttttg aatattctgt atttattttt caaagtacta
    16621 acctaacatt gaaatttgat aactatactc tttttctata tctggtaata tgttatttgt
    16681 tttcaataag tcatataaaa acaatcttcc cttttgagtc cacttagttg tcatttttac
    16741 atcagtcatt ccattacttc tagttatgtc tattgtttct gaatgagtgt atcccttccc
    16801 ttgatgttgt ttatataaaa gccattgtcc actttgttta tattgaatcc ctctttcatg
    16861 aagtatttta ttcatttctt ttccactcat tccatagtct tttgctattt gagttattgt
    16921 gacaagtcct ttatttttta atatcatatc tgtataatct gcctttggtt ttagttcttt
    16981 tattacttgg tctttcattt tactttctag ttgtaactgc tcatttactt ctacttgctc
    17041 tataagatgt tgtaacgctt ctttatatgt agttggtagt ttagtttgca aatctatcat
    17101 attatatgtc ccagtactac gtatacttgg taatatctca ttagttaccc aacgtttaaa
    17161 taattttgca cttggcaact tagaacttaa tattaaagaa taaagaccac tttcattaat
    17221 tactttcata ttttgattac caccaggagt cggtatttca cctactcctt tatcttcatc
    17281 atcaacatgt cttttcaaag catcagatgt atctttatat cctaattgtt ctgcaatatc
    17341 ttttccaaca aaccaaaatt ctccatttag ttcgattact cttatttctc caaaagtctt
    17401 atttttgaat atttgtaaat tattcataat ttctttcctc cttatgattg tttttgtgaa
    17461 caataaattt taatttatgt taaccttttt tctaaaaaaa gttcatccat tgttttattt
    17521 aaaatatagc taatcattat catttctttt tccgtaaaat ttctttttcc attttctttt
    17581 agattatatg ttgattttgc tattcctatc ttatatgcta gttcttcttg ggtatatcct
    17641 gcttctattc gaccagcttt taaacttctt ttcttcatgt tcttcacctt ccaatgtttt
    17701 atttttgtga acttcatact tttattatat tcacacaaac aaaacttgtc aatacataag
    17761 tttatttttt tgttaacttt tttatttttt tctaaacata agtttacgtt tctgtaaact
    17821 tatagtatca tttaattatt gggaggtgtt gtttgtggca acttttggag aacgtttcaa
    17881 acaattacga gaagagaaaa aacttactca agatgaacta gtttcaaaat tcaataaagt
    17941 ttactttaca agttttaata aatcaacaat atcgcaatat gaaaataata aaagaaaacc
    18001 agaaataaat attctagaaa attgggcaga tttttttgat gtatcaattg attatctttt
    18061 gggtagaact cttgttagaa atcacatcga tactgtagcc gcacacagag ttaatcctca
    18121 caaagattta ccagaggaag ctcaagaaca actcaacgat tatattgaat ttttaatgaa
    18181 taaatataaa aaataaggat tgaatctatg ttaatattaa aaaaattgag aactgaaaaa
    18241 ggaatttctt tagataaatt aagcacagac ttaaatataa ataaatccac tttatccaga
    18301 attgaaaatg ggttaagaga accaaaagaa tcatttatta aagattgctc agactatttt
    18361 ggagtatcta cagattactt aattggaaaa attaacatag atgattcaaa taaattaaca
    18421 aaatgcttaa attcaacttt tccaataagg ttaaaagaat taagaaagaa aaaagaatta
    18481 actcaagcag aattatcaaa attacttaat tgttcactaa gcaaaattgc tatgttagaa
    18541 actagcaaaa gagaacctgt taaagaagac ttgttaagaa tttcagaatt ttttaatgtg
    18601 tcagttgatt atctacttgg taaaacctca attgaaaatt acataactac tgatgaaata
    18661 tccaaaataa ttaaatctta tgaaagttta cctaaagaag cgcaagaaca tattaatagc
    18721 tacatagagt ttttagttga tagatataaa aaatgaatat ttagagcagt tcactcctgc
    18781 tctttatata taaaaaagca acacatacat tctttttata gggggatttc aatgaacaaa
    18841 ctagacgcac ttttagactt agcaaataat gaagagatag aaatttacta cactgacaaa
    18901 atagcagatg acataaaagg attgtatata aacagacaag gactaaagat tatatcatta
    18961 cttaattcat taaaacaaaa caatgctaaa ctaatagaaa tcttagcaga agaattagga
    19021 catcatttta ccagtgttgg gaactatgta tcttcaaaaa acagttacaa aaataaaatc
    19081 ttgatagaca aaactgaaaa caaagcatta aaatgggcat gtgaatttct tataacagaa
    19141 gaagaaataa tacatgttat taattcacac gctacaagtg tatacgaaat agctgaagaa
    19201 ttacaagtta gcatcaactt cttactaaaa agattagaat ttctatcaaa aaagaaaagc
    19261 atgttggact taggaaataa tagattttta gtattaacta atttgccaaa tttctacata
    19321 tatgaggata ttttttaaac tcatttattc tacttttata gattttttac ttaataaata
    19381 tatatttcaa tattattata ataaactaca cataaaagct aaaaaattgt aagaatatta
    19441 agaaaataat taagtaaaaa ccagataaac aaaattaaga taatattgta cataacaaaa
    19501 gtatataaag agcagttaat ctgctctttt atataaacac caaacataca tacattctaa
    19561 aagggagaga tactattatg aaaggtggag taagaaaaag aagcaacaaa tggtattact
    19621 actttgacct aggcatagta tctgaataaa gcaatatcag cttacctttt ggtacaaaaa
    19681 atatttctta cttaacaaca ctcaaaaaaa ttaccaaata aatatagaaa actaaaatta
    19741 agtatttata aagtaaaacc attatacaca gtagtaattt tgatgagtaa acacatattc
    19801 catatgtaat aaagcttgaa gttaaaagtg gaataaataa aatctagaaa aaatttttat
    19861 tttaagttaa gcttattatg gataataaaa ttcttgcaaa tttagaatca aatgatacaa
    19921 taagaatata aaatacttgt ttgagaggat tattttcgtg attaaaaatt ttgatgaatg
    19981 gaaagagtat gatggttttt cttatggtag tggaagaagc gagaaaatct ggctaattaa
    20041 tgaaaaaact aaagatatag gcttgtttaa attccctaaa acaaaagaaa ctggagaata
    20101 ttgggcagaa aaattagcct gtgaaatagc tagtttgtta aagattcctt gtgctaatgt
    20161 tgagataggt acatataata aaagaattgg ttcaatgagt tatttaatca ataaaaatga
    20221 tgagaaattg attgaaggcg tgtggttaat aaacaaatat tatccttgtt ataatacaga
    20281 taagttatat gattataaaa ataatgaata ctattcattg aaaatgattt ttaaatctat
    20341 agaagaatat aacataaaaa atgattttct aaaaatgtta atttttgatt acttgattgg
    20401 aaatagagat aggcatcaaa ataattgggc tctactagaa agtcaaaata aaattactct
    20461 tagtcctcta tatgataacg gatcatcatt atgttgttat gttaatattg agaacataga
    20521 ttctttcttc actgatcatc aaagatttga agctcttata aatacacgtt caagatcaag
    20581 aataaggata gatggaaaaa ataaaaaaga accaacacac ttagaagtgc taaaatattt
    20641 aaattcaaat tattatgaac aaactataga ttttgtagaa aagataataa atcttatgaa
    20701 tgaagtaaac ataaatacat taatagataa ctattcagaa aatattataa gcccaaagtt
    20761 aaaaaaattg ctaaaaatat atctaaaaga aaaagtgtta agaataaaaa atatatataa
    20821 tttgtgaaag agggataaaa tgtcaattaa aaatggaaaa gattttttat atgttatttg
    20881 gaaagatcca aaaagtagaa gacaatttat aattggaaag ttagctaaaa atggattgta
    20941 tgaattttct tatgaatatg aaattgatga agctattaaa gcaggtttta atgctttaat
    21001 atcatttgat gatattagta aaacatatta tagtgaagat ttatttagcc catttgcaag
    21061 tagattacct gataaaaaaa gaaaggacat caataaaatt ttggataagt atgatatgtc
    21121 tgagtatgat acatatgaac ttttaaaaag aagtggtgca agattaccta tagatacact
    21181 agaatttgta gatccaattc ctttggacat tgataaaaat ataattagat atttttatat
    21241 tgcaggagta agacattatg ctgggtgtga aggacatgaa tgtgataatt caatagaatt
    21301 agaatcaaat gaagctctag ttctagagtt agaaccagaa aataaatttg atcctcatgc
    21361 aatcaaggta tttgataaaa aagagaatca tataggttac attcctagat attatagtgg
    21421 agaattaaca agtataataa atagaggatt aaagtataat tgcattatta aatttgttaa
    21481 caaagaaaat aattgtgatg aatgtataaa agttaaatta gaaatattat ttgacgaata
    21541 taattaatgt cttcttgtaa atataggaga cattttttat ataaacacca aacaaacata
    21601 cattctaaaa gggagggata ctattatgaa aggtggagta agaaaaagaa gcaacaaatg
    21661 gtattactac tttgacctag gcatagtaga aggaaaaaga aaaaaagtag aaagagttgg
    21721 tggcaataca aaaaaagaag cagaaaaagc actaagagaa gcactaaatg aatatgaaaa
    21781 ctctggcata gtctttgaag aaagtaacat cagcttatca gactacctag acttttggta
    21841 caaagaatat gttttactca actgtaaata caacactcaa gaaagctacc gaataaacat
    21901 agaaaaacat ataaagccaa aactaggagc ttacaaagta aaagctttaa ctcctgcaat
    21961 actacaaaac ttcataaata aaaagtacaa agaggattat tctcaaaata cattacaagt
    22021 attaaaagcc atattacata ggtcattaaa atcagcagtc catccttaca aacacatacg
    22081 agaaaaccct atgcaatatg taagcatacc aaaaactaaa tctaaaacag aaactaataa
    22141 agttaaaact attacattag aagaatttaa tcaaatacta aatatatttc ctcaagattc
    22201 atttcaacgt atagttttac taattggatt tcatactggt atgcgaagag gtgaaattat
    22261 tgcactaaaa tgggataata tagaccttga taataaaact atcacagtaa agcatacttt
    22321 gattaaaaaa ccaaatggaa tgtttgaatt agggcaacca aaaacagaaa gctcttgcag
    22381 aactatattt acaggtgaca ctttaataaa ggcattaaaa gaacataaat tatatcaaaa
    22441 gaaaatgaaa ttaaaatacg gagaatttta ctttgatagt gattgggtat gtaccaaaga
    22501 aaatggtcaa caagtgaata ctcacacttt agacactata gtaagacaaa ttcgagtagc
    22561 tttaaacaat gacttccatt ttcattcttt aagacatgca catgctactc tattattaga
    22621 aaatggtgct aacattaaag acatacaaaa ccgtttgggt catagccaat tatcaactac
    22681 aatggatacc tattcacatg taactgataa aatgaaaaat gaaactgtag atatatttga
    22741 aaaaattaca aattagagtt tgccacccaa aaatataata cggtggcaaa tgggtggcaa
    22801 aatctaattt atctatttta aaagctaaaa ttatcaaatt tatatagtca gctatacgct
    22861 tgtaatttca aggctttaga gtatataaca accataacta atataaggta ttaataataa
    22921 atctaacgaa taaaacttaa tatttactta agttgtaata ctattctaaa ttgtataatt
    22981 aaaatttaat aagttcttaa ttatatattc attgatttat ttgcaaaaat aaaaaatgcc
    23041 aatctatctc taaacaaaaa ctgaatacca ataaaaattt aataaaaaat tttatcaata
    23101 ttcagtataa gttttagtta catatcaaat tttccaatgc atcattaggt gctacaatag
    23161 gataaacatc ataatcctta tctatatcac attgtaaaaa tacaggttta ttaaaatcta
    23221 tcacatctag aacctttcct aattcttcca tattggatac cttatatcca tcaatattat
    23281 atgcatttac gagtttaaca taatccacat tttcattaat atcagtttga gagtatcttt
    23341 gattggaaaa taacttctgc cattgtctta ccattcctaa tgttctatta ttgagtagta
    23401 aaataagcat aggtacatta taatttgcaa ctgtagctag ttcattacag ttcattctaa
    23461 aacttccatc accagttact aaaaccacat ttttatctac attaccagct ttagttccaa
    23521 tagctgcacc taaaccaaat cccatagttc ctaaccctgc tgatgttata aaacttttat
    23581 tacctttaaa attccagtat ttagcagacc acatttgatg ttgaccgaca tcagttacta
    23641 ccacagtggg tttctttaat gtttcatatt tttcatttat ttttttaagt atattttgtg
    23701 gatgaaattc atatgtttgt acaccctcac tctttctaaa tgtctttatc tcttctttcc
    23761 aattgctatt attcttactt tcaacccttt caattaataa actaaggact agctttacat
    23821 ctccaaccaa agatacatta gattctatat ttttacttat ttcagatggg tctatgtcta
    23881 tgtgtattat ttttgcattt ttagcaaatt cagaactttt acttatcact ctatcactaa
    23941 atctcgcacc tatcgctatt actaaatctg aattagaaag tgctaaatta ctttctctac
    24001 ttccatgcat tccaaccatt ccaagagata gttcattctt tctatctata tttccaagcc
    24061 ccataagtgt atttagtacg ggtgtatcta tcttagttgc aaatttttca agaatttcct
    24121 cactgtctga agatttaaca cctccacctg catatattac aggcttttta gattctttta
    24181 tgatatctat tgcttcattt aaaagcttaa tattagtttc atcatctaga tcaaaatcac
    24241 tcttataatc catataatca tctatttgac ataaatcata gtcctcacca ctaaaatcca
    24301 tttctgctaa aaataaatct tttggaacat ctacaagtac aggtcctttt ctacctgaat
    24361 ttgcaactct aaaagcttct tttattgtag gaactaattc tttagtgttt ctaactaaat
    24421 aattatgttt tgtcatagat aatgttgctc cagttatgtc aatttcttga aaagaatctt
    24481 ttcctaataa actagttggc acctgccctg aaattacaac cataggtgaa gaatccataa
    24541 aagctgttgc tattccagtt atggcatttg ttgctcctgg accagatgtt gtaaaacaaa
    24601 ctcctactgt attagtactt cttgcgtacc catctgcagc atgaactaaa ccttgttcat
    24661 gggatgtcct tatatgttta aaatcatctg aataatcgta tagagcatca taaagaggta
    24721 tcacggctcc accaggatat ccaaatattg tgtcaattcc ctctttcttt aaacattcta
    24781 aaattacctt tgctccattc atacgcacta ctatacacca ctctttccct caaactaatc
    24841 catttaatat atatttattt taagaatgac atcatacttc ttagatttct accaacttcg
    24901 gcaatttctg tattatattc ttcttctcta gtttttagga agttttcacg tccttcttca
    24961 ttttctttaa tccacgcttt tgcaaatttt ccattttgta tatcctctaa aacattttgc
    25021 ataccttgtt ttgcagcatc agttattacc cttttaccag atacataatc tccatattct
    25081 gctgtgtcac ttatactata tctcattctt tcaaatccac cttcatatat aaggtcgact
    25141 attagtttca tttcatgtaa acattcaaag tatgctactt ctttttgata acctgcatct
    25201 acaagcgttt tatatcccaa ttttataagt tcacttattc caccacaaag tactgattgt
    25261 tctccaaata aatctgtttc tgtttcttct ttaaaagtag tttctaaaac acctgctctt
    25321 gttgctccta tcccttttgc atatgctaaa actgtctctg tagctttttt agtgtagtct
    25381 tgatatactg caaatagtgc aggaactcca cttccctttg taaatacatt tctaactaag
    25441 tgaccaggtc ctttaggagc taccattaca acatcaacaa attctggtgg ttgtacttga
    25501 ttataatgta tattgaatcc atgagcaaaa gctaatgtct gaccttcttt taagttatcc
    25561 tttacactct cttcataaac tttcttttgt ttttcatcag gcattaacat cattgtcaaa
    25621 tcactttctt ttgtagcttc tgctacactc ttaacttcaa atccatcttc ttttgctttt
    25681 tgtgctgatt tacttccgtc ataaagacct atcactacat gtacaccgtt atctctaaga
    25741 ttttgtgcat gtgcatgtcc ttgacttcca taacctaaaa ctgcaacctt tttatttttt
    25801 aaaacctcta aatccacatc tttttcataa tacattcttg ccattttcat ttcctcccaa
    25861 tactttttct tatttttatg atatttattt ttattagaaa ttcatactca ttatcattgt
    25921 tttaatatta ttcaacagat ttaaattcta aatttatata atcttacaat tataatttta
    25981 gaattttcag tttttattag ctacaacaaa ctaataaatt tattaactgt gataaactga
    26041 taaattcaat gacacttata ttttaatcta agatttttct tatttaatat ttatctttag
    26101 ctttataaaa tatatttaat taatgtctca ttctttcaac agttatctct cttatatcat
    26161 gtagtttttt catatggttt aagacagaat ctaaagatgt cttttcttca tttactatta
    26221 attttatacc tgaattattc atattatcta cagtcatact tattgattgt atatcaacct
    26281 ctcttcttct tagagtcata gccactctta aaaaagaatc tattccttgg tttaatttag
    26341 catatacagt attttccata aaaatccctc cttattttta ttattttttt attaatttta
    26401 tatggattaa atttatttat atcgcttaaa ataaaaaaac tttcatcttt ataagcaata
    26461 taaatattac ttataaggac gaaagttata ttccgcggta ccaccttatt tgtagatata
    26521 aaaaatacat ctacccctct tttttcaggt cgggtaatta attaccttaa ccttagtcat
    26581 gtaacgttga catacgtttg tacttactaa aaatattcag tacaaaagct ccagagtgat
    26641 cattatacta ctgttaacac caattctcag caacattggt tctctgtgga taacactatt
    26701 agtattgttg tctctgtcat tgcttttaat tttttaattt tatattttaa ttctgttttg
    26761 ttgttaatat agattatatt ccctatcatt tacaatgtca atgacttttt ttaaaatttt
    26821 cagattattt atttttttca caataatact tcttttatac tttacgcctg tattttggaa
    26881 taatcgtttt cagattatta ttttatatcc atttattttt agccaatttt ctttatcttt
    26941 ataatcgggc ataatggatt ttactaaatc atagaatttt ttagagtgat tcatatgtac
    27001 taaatggcta aactcatgta ttattacata atcaattaca tctaatcttg ccatagatat
    27061 ttttgagttt atataaatat ttttttgagc tgtacaactt ccccatctct tattttgttc
    27121 ttttacctta acacttgctg gaactaattg cttcattatt tcacaattta atttaagaaa
    27181 atctattctc tctaatactg ttctttcact ttccatctta taccaagatt ttaaagattt
    27241 tttaataaaa tctgtacttg tatcatttgt ctgaacaact atttgatatt cgttaatagt
    27301 tattctacag ttactttttt tgacagaatc tttcaacagt tcttttataa ctagatggta
    27361 ctcttcacct aaataaagaa atttttcgcc agttataaac attctttgtt tgtatacttc
    27421 ttctcttcct ttatattttt ctgacttttt aagtatccag tctccttttt caataattat
    27481 atcttttatt atttttttgg atattttagg aggactcatt acaattattt tgccaaatgg
    27541 atctattttt atagaaatat tttttctatt tttatatact aaaactgctt ttatttcaat
    27601 ccctctaaaa ttaacattta aagtatcaat atctttactc cctgtgttca ttagtttatt
    27661 tttccttatt agctctacaa tttataagtt cagcagctat acatacagct atttcagctg
    27721 gtgtttgagc attcaatttt atgcctattg gtgcatgtac ttttgataat tcttccttct
    27781 taaaaccttt tttcatgagt ttttcgtacg tcaatccaat ttttgttcta cttccaatca
    27841 tacctatata accagtttct cttttaagag cccattctaa agcacttgaa tcatatagat
    27901 gacctcttgt taaaataatt atatagctat tttcgtcagt tggaaaatct ggtatatttt
    27961 caaaagaatc taaaactact acttctgatt ctgggaacct ttcataatta gcaaattctt
    28021 ctctatcatc tattactact gttctaaatt ctaaaagaga tagtatcaca gcaacatctc
    28081 ttgaaatatg acctgcacca aatatatatg ctttacttgt attttccttt agtctcttat
    28141 tgaatttttc tatattactt tttaatttac tatctataaa atctatttgt atttctccag
    28201 tacctccaca tatcattcct agttttgcca catcagattt gtttagagta aatttataca
    28261 acattgattc tctatttttt ataacattaa ttgcatgttt tatacattct gcttctacac
    28321 tacctcctcc tactgaacca aatatagtag aatcattttt cacaatcatc attgctccag
    28381 tttcacgtgg tgttgaacct gaatgagacg ttattgtagc aaatgcgaaa tcttcattat
    28441 tttctattaa tcttattgct tgttcatata tattcatata attctcctta ccagtctatc
    28501 taaaaatctt ctatatatct atcttagtct ttttattact ttatttggta tcacaaattc
    28561 tactgctccc atataaccag gtactacttc atattcttca aaactaatca caagatttcc
    28621 atttttatta aaataaaaat cttgatactt atttatagat tcaaaatctt tcactgggaa
    28681 gtctttatct tggtctataa aataagattt tgtatcatct ttcttaattt gatttctcat
    28741 ctgctctttt atattatcac ttatagctct aatatatcta ctatctttaa aaaatattgg
    28801 taaatttaat agtattcctc tatttttatc aacagtgtaa tgcttcttag ttatagacga
    28861 ggaatcttct atctcttctt ttgtcatttc tattgataaa aagtccttag aattattttt
    28921 gacattatgg attacattta ggtatctatg acccttatta ttttcctttt tcattttatt
    28981 ttgaaattca ctgtaaattt ttttcccatc ttgcaaaaac tcattgttta atttattctc
    29041 taaagtttta tcttttaaac cttttatttt aggaatactc acattcactt cataattgcc
    29101 ttgtttaaag ttgagactct tagcatttaa cacctttatc gtatcactat ttgaagtctc
    29161 attagaggaa ctacctgcaa atatagggtt tatatctact aatgagacta ataagcatat
    29221 tgaaaatata gaaattttaa atatagattt tttaagcttt ttatatgtag tcatattgtc
    29281 cttcttaaaa tatgtatata taaactacta ttcaatttac aaatctttta ttactttata
    29341 tggtattaca aactcaactg cacccatata accaggtgct acttcatatt catcaaaaca
    29401 aattacaagg tcttcatttt tattaaaata aaaatcctga tacttattaa ttgttttaaa
    29461 atcttcaaca ggcaagtctt ttttttgatc tataaaatag gattttgtac tatccttttt
    29521 catttgttct ctcatttgtt cttttatatt atcacttata acttttatat acttatcatc
    29581 tttaaaaagc attggcaaag tcaaaactat ttgtcttttt ttatcaatag tataatgtgc
    29641 cttactcaca tatgatgaag cctctatctc attttttgtc atctctattg atagaaattt
    29701 tttagaatta ttttttacac tataactcaa acttaaagat ttatatcctt tattctttga
    29761 tgattgtatc ttctccattc tctcttggaa ttgatgataa agcctttttc catcttccat
    29821 aaaactactg tttaatcttt gttctaagtt tttatctttt aaacctcgta ttttaggaat
    29881 atcaatctca gcttcataat tttcactttt taaactataa ttttttatag ttattacttc
    29941 tactattact ccaattacag gtatcttaga aaaattgtct gcaaatacag gacttatatt
    30001 tactaatact gtaaatatac atgcaaaggc tattgcaact ttaaatctta atctatttac
    30061 ttttttattt ttctctcgat atatgttttc tattttttca tgatttatta tagtatctaa
    30121 tttcttgggt attggaattt tatgatactc atcttttaac ttatttaacc tttctttgct
    30181 tgtcattctt tttatcccta ctcttcaatt tttaatttta gtttttttaa agcagtatat
    30241 aacctagttt taacagtatt cacattttca tccaatatta ttgctacttc ttcaatcttt
    30301 aaatcttcaa aaaatcttaa tattataata gatttgtatt caataggtaa ttcttccaaa
    30361 gctcttctta aatccaaatc agtatattta tcatactctc cagtctcatc tataaggtca
    30421 atatctgtca tattattata tttacggttt tttctaataa aatctatact cgttctaact
    30481 aaaattttat aaaaccatgt ctttatataa tttacttctt taatattttc cactgagtat
    30541 agtgccttat acatggcttc ttgaaccaca tctaaggcat cttcttcatt cttcgtataa
    30601 ctatatgcta ttctataaaa agagtcttta ttatcgataa tgtatttctc aactttcgtt
    30661 ctctttaaac tcttaaacat tcctgtaaaa tctccctccg attacaggct aggattgagc
    30721 tgttagaaat tctatctgtc tattttagca tcttcattca gtttttttat atgctcaata
    30781 tacttttcat ttaaaataga ttctcttatt aaactctgac actcattaaa acttttttgt
    30841 ctttctcttt tatcagtaac tttgactata tgatatccat aactagtttc aaacacattt
    30901 gaaacttcat ttttctttaa tttgaacact tcctttgtaa actcagcatt tttatcatcc
    30961 tttgtaaaat atcctagctg tcctccattt tttccagttg ctttatcatc cgaatatttt
    31021 ttagctaaac tttcaaaact ttctccattt ttgattttag tcaatatatt atctgccttc
    31081 ttcttcaaag cttctttttt atctttgctt acttcttttt tatttttatc taaagtcgat
    31141 ataagtatct gagaagcact tacttcttct acattaaaat cttttttatg acttgtatag
    31201 taggcttcca tatcattatc acttatgttt attctatctt caaaggcctt tttattttta
    31261 tctattgcta aatctctaga tatttcttgt tttaaatatt ctttatcaac acctgctttg
    31321 tccatttttt ccttatagct tgtattcaaa ttcatcttat cttctagttc ttgatatttt
    31381 gagtctactt cacttttact tggagttagt cctttatcct tagcctcttg atataaaagt
    31441 tcattatcca ccataaacga tattataaca ttttctaatt tatttctacc ttttttatcc
    31501 aaagtttttt ctaattcatc taaagactgc ccatttatat aattattagt agcagataaa
    31561 actgcttttg tttttttgta ctgttctttt gtgatttcca catcatttac ttttgctaaa
    31621 cttttattac aaccaaccat catcaaagac aatattccga ccattcctat ataaagtttt
    31681 ttattcaata tacctacacc tcactcattt ttattctcaa tttctaaaaa tatcaatcaa
    31741 tattcttata ttttaactac tctaaattac gtaaccaata attttttggt atttggtttt
    31801 gatctactgg cgcaaattca taaccttcat ctgataagaa ttttaaaact gaatctaaag
    31861 cttctgcact ctgttttttc tcatgcatta aaactacaag ttggtcttta tcttcacctt
    31921 ttctattctt aatatgattt tttacatttt gtacaatttg agaagaatta gatctccaat
    31981 cttctgtatc taagtcccag tcccaaattt tatatccagc atcaactaaa gcctgataag
    32041 aggcttgtgg tgtatatggt ttgcttccat aaggtattct tattactttt gaatgtttac
    32101 cagttatctt ataaaaagtt tgatcatttg tatcaaattc ctcttttgct gatgtactag
    32161 taacataaag cttgtgaata tcatgtgata cactatgaaa acctgctgtg ttcccatttt
    32221 taattatatt tttaacttgc tgtggatatt ccttcatatt cccatctatc ataaaaaaag
    32281 ttgctttaac attgtattta tttaaagttt ttatcatctc gtcagtaaat ttagatgggc
    32341 catcatctat agttatgtat gctatttttc cagaactttt acttggccct ttactaactt
    32401 ctattgatgt attagatact atatctgtaa tagtagaact tgacagtttt gcatttctac
    32461 taatttcatt ttccttcata tttaaagagc tgacgcgaac accaactact ataaaagcca
    32521 gacacactac aatagcactt aaaagcaaat acaacttctt tctatttatt tttttctttt
    32581 tatttctcat cgttcgacct ccaatattac tatattttta catttatatt tttgtagtat
    32641 tttatcccaa aaatttacac tacatatata tagacgttga acttcgacaa aaagttttgc
    32701 ttaattttta aaatttgtta tttcggtgat tattatgctg tataatttct gtataattgt
    32761 atagaatgtt gaaaattgag tattatatag tgttttttac
//
LOCUS       DAGQWR010000029        32374 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.029, whole genome shotgun sequence.
ACCESSION   DAGQWR010000029 DAGQWR010000000
VERSION     DAGQWR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.029"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            770..2440
                     /locus_tag="MIW95_001174"
     CDS             770..2440
                     /locus_tag="MIW95_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBY3150518.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2562..3296)
                     /gene="pflA"
                     /locus_tag="MIW95_001175"
     CDS             complement(2562..3296)
                     /gene="pflA"
                     /locus_tag="MIW95_001175"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBY3150519.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3456..5687)
                     /gene="pflB"
                     /locus_tag="MIW95_001176"
     CDS             complement(3456..5687)
                     /gene="pflB"
                     /locus_tag="MIW95_001176"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBY3150520.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFS
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5980..7041
                     /locus_tag="MIW95_001177"
     CDS             5980..7041
                     /locus_tag="MIW95_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBY3150521.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGRKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7132..8745
                     /locus_tag="MIW95_001178"
     CDS             7132..8745
                     /locus_tag="MIW95_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY3150522.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9020..11005
                     /locus_tag="MIW95_001179"
     CDS             9020..11005
                     /locus_tag="MIW95_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBY3150523.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLSFGILEKPVVWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMIEELAKVKSFDEFYKIITKRR"
     gene            11010..11444
                     /locus_tag="MIW95_001180"
     CDS             11010..11444
                     /locus_tag="MIW95_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBY3150524.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11473..12033
                     /gene="srlA"
                     /locus_tag="MIW95_001181"
     CDS             11473..12033
                     /gene="srlA"
                     /locus_tag="MIW95_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBY3150525.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12351..15401
                     /locus_tag="MIW95_001182"
     CDS             12351..15401
                     /locus_tag="MIW95_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBY3150526.1"
                     /translation="MKKMLKRLCTGFLALATVVTALPTTPVHAESKQYWTESAERVGI
                     IEKVMNDGSIGSTFNEGYMKVEGETAYCIDINTNFKNGYKTRADASSRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHFGWQCDNVRASYDEIPKATQDE
                     VFSGAKAFIKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDENGNTDVVEVKAGTVYIKELSAPAGYKVDKT
                     IYSLKVEVGKTAILKVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYIAKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKAPNGYLLDGAYMQAGDKSEQIKGLYVTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNAVLVEGKLYKKNEVVKTIH
                     TDIEGVASTSSDLLPYGKFRIVESEAPDGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLANVPFRITSKMTGENHVVVTDDNGQFSTSAEWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEITIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENVELLINGKRVENDYTFVADDEEMKVEISYTFNASALGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIVKIHTTATDKDGNKELEAGKDVTIIDTVI
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVKSDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKTKVPEEPKKPETPQTPD
                     TPHKTDSPKTGDSTNLYGLLALLLTSGAGLAGIFFCKRRKMKKS"
     gene            15638..15964
                     /locus_tag="MIW95_001183"
     CDS             15638..15964
                     /locus_tag="MIW95_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBY3150527.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPVFERDKNNHRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLLADDI
                     VAVEEK"
     gene            15980..16363
                     /locus_tag="MIW95_001184"
     CDS             15980..16363
                     /locus_tag="MIW95_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBY3150528.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKSNQNAGNTQNHTPQGQPKK"
     gene            16465..17229
                     /locus_tag="MIW95_001185"
     CDS             16465..17229
                     /locus_tag="MIW95_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY3150529.1"
                     /translation="MDSWFTVEQIDQDTFVISEYKHWEETHCYLLCGTKRAILIDTGL
                     GVSNIREVIDGLTKLPVTVITTHVHWDHIGGHKYFRSIAVHEAEKEWLSVKFPIPLQV
                     VKNNIMCNPCDFPSDFKVEKYQIFQGVPQMILHDEDCIDLGNRKLVVIHTPGHSPGHC
                     CFYEADRKYLYSGDLIYSGCLDAFYPSTNPQEFWKSVRKIQSLKISRILPGHHHLSIP
                     VTIIDKIETAFHNLSNEGKLKQGNGIFSYEGVQIHI"
     gene            17328..18722
                     /locus_tag="MIW95_001186"
     CDS             17328..18722
                     /locus_tag="MIW95_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY3150530.1"
                     /translation="MRMIWNKGHRIRASDKHLVYHFSIETLLFVFVAVLLLLNSKQLM
                     RTDWEHFSLLDNGFTLSLYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEKMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAQARQ
                     DGTATCEAKGDGTD"
     gene            18906..20102
                     /locus_tag="MIW95_001187"
     CDS             18906..20102
                     /locus_tag="MIW95_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBY3150531.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLNTLHKELAKFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     NYGRYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPA
                     KDLVES"
     gene            20115..20249
                     /locus_tag="MIW95_001188"
     CDS             20115..20249
                     /locus_tag="MIW95_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBY3150532.1"
                     /translation="MWVLLKDFLLVSMGMGIGVVLMCILNVGKEADYEMEQLKESEDN
                     "
     gene            20250..20471
                     /locus_tag="MIW95_001189"
     CDS             20250..20471
                     /locus_tag="MIW95_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150533.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            20561..21181
                     /locus_tag="MIW95_001190"
     CDS             20561..21181
                     /locus_tag="MIW95_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150534.1"
                     /translation="MSNIKLRNTYKSYTAIFVIGILVGCICRLLDYFPADTLWSFSSP
                     QTLFGFWIITNTIIVMLSTSNICAGISSFLYMFGMTLSFYALQAILGMFIPMFSGGFR
                     FGLFILFTVWSIACAIAAFILYYWNREHIFSSVLYSLPVGALFAETIAVFIYFLEHQT
                     FLFQLLMDIIGAVVFTIIFFKKVNYRKMYIVGIVLSTLVFYYIFPW"
     gene            21291..21587
                     /locus_tag="MIW95_001191"
     CDS             21291..21587
                     /locus_tag="MIW95_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBY3150535.1"
                     /translation="MSDIFKDMQTKVGCDYISDLPSYKRKVWQEMKRLNPADYEERQL
                     EDFSKYVFGMSYQTLQDVMKQQKGREEQCRKQGCWWKRKEQLAKKQHHTGLTCR"
     gene            21506..22009
                     /locus_tag="MIW95_001192"
     CDS             21506..22009
                     /locus_tag="MIW95_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBY3150536.1"
                     /translation="MQETRVLVETKGTTGEETTSYWFDLPIDVAEFEEKLGVGAESGD
                     YRIIEKVLPFADEVHEHTSVYQLNELDFMYRQLSSDMQEEYVSLLTVYENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            22027..22530
                     /locus_tag="MIW95_001193"
     CDS             22027..22530
                     /locus_tag="MIW95_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBY3150537.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            22647..23045
                     /locus_tag="MIW95_001194"
     CDS             22647..23045
                     /locus_tag="MIW95_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBY3150538.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFLI
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            23023..25473
                     /locus_tag="MIW95_001195"
     CDS             23023..25473
                     /locus_tag="MIW95_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY3150539.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLKEVACQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIHDFGYLMEHLYGRDGIAYEDYEYQLPKKKLQKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDRESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVVRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQAVILDPKAERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSDSRGLLHVIDELRREDTPISRNIAEHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            25473..27677
                     /locus_tag="MIW95_001196"
     CDS             25473..27677
                     /locus_tag="MIW95_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3150540.1"
                     /translation="MKERIKGAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     IVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SVSPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQNMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRAERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHRQNIADKRMELQKAQEARQRKTDGSATTGATRPHERPA
                     TASTIPKPSAEKMQEVKRPATATTSKASEPVKTNVVKERPLSSGASDKKATQPAQPVH
                     RQNVEKVVSQETRQNYKAERTTKKQTFEQSKRTTEHTEKNRNLVTKKGQKKK"
     gene            27674..28681
                     /locus_tag="MIW95_001197"
     CDS             27674..28681
                     /locus_tag="MIW95_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBY3150541.1"
                     /translation="MKLKHIALIGSLFPILFSIVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYQYGNQFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGILVSPTQMYHAGDPIGYADLSSSYWQQHLIGAGRVK
                     Q"
     gene            28699..29610
                     /locus_tag="MIW95_001198"
     CDS             28699..29610
                     /locus_tag="MIW95_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBY3150542.1"
                     /translation="MFRKNKKQTETIKEPKEKKVRTFKVGTHKKSVIALWVVLIASVS
                     FGAYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKPYYTWSNSKEAIEARTQA
                     ISGYLTKELQDLNVDTIRTDIPTSSTVTDVIVWSIEQSGTDTFSATYEVDQQIKEGEQ
                     TSNVKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDYEPKTPEANASVDADTVNDATA
                     FLETFFKLYPTATEKELAYYVSGNVLEPIGRDYIYAELVNPIFTKDGDNVKVKVAVKF
                     LDNQTKATQISQYELVLHKDSNWKIVG"
     gene            29711..29914
                     /locus_tag="MIW95_001199"
     CDS             29711..29914
                     /locus_tag="MIW95_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3150543.1"
                     /translation="MKNRLEELRKQRGIKQEDLATALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESK"
     gene            29911..30327
                     /locus_tag="MIW95_001200"
     CDS             29911..30327
                     /locus_tag="MIW95_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150544.1"
                     /translation="MKHKIQKVELFIGVIIFLGGLLTYGLGVHQLLPVPRPDLVVYGT
                     TLIGIILIFMGCDIFSKPTKEMQILENDERNIAITNASLANAYKVTLTLLVLVLFALV
                     FMGYMSKVVFFSIAGVIAIGQITWVITARYLDKNTS"
     gene            complement(30505..30789)
                     /locus_tag="MIW95_001201"
     CDS             complement(30505..30789)
                     /locus_tag="MIW95_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HBY3150545.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYPECKFTLAEHFR
                     KKKLTKTNVKELLEGKETLVKGIKNKEKKPYNAVVKIGEKGYIDFISFSK"
     gene            complement(30935..31672)
                     /gene="erm(B)"
                     /locus_tag="MIW95_001202"
     CDS             complement(30935..31672)
                     /gene="erm(B)"
                     /locus_tag="MIW95_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBY3150546.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(31797..31880)
                     /locus_tag="MIW95_001203"
     CDS             complement(31797..31880)
                     /locus_tag="MIW95_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBY3150547.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            complement(31929..32170)
                     /locus_tag="MIW95_001204"
                     /pseudo
     CDS             complement(31929..32170)
                     /locus_tag="MIW95_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
BASE COUNT        11004 a         5226 c         6464 g         9680 t
ORIGIN      
        1 agtgtaatcg tattttttat taattcttat aaaatcaatg aaaataagga tatccatttt
       61 aaagtacata aatttaaaaa tccagaaaaa tatacttaaa ttatgtaagc aaaagagggg
      121 ttgaatcggc tgaaaatgta atactagtaa atagtacatc aatcctagat accctgtaag
      181 caactctatt ttcatgtact aaaaatgtac ctattttatt aactgaaaag aataaattaa
      241 atgtaagtac ataataagag ataaaaaatt aaaagcaaaa aatatttata taaataaata
      301 taaaaaatga tataatccct attaaaaaat ttatagatga tgaagaaata tcaaaatctt
      361 atattatagg aagaggcact tctttgccaa aatatataga aaagcaatac caaatccaaa
      421 gagaataggc tgagaaaaaa taaaacaaat tcaagagtaa tagaaaagct ttatagaata
      481 aagaactgag aactctttat ataataaaaa atggactttt aagagaaaat gatttaataa
      541 atgatttaac atggtgtttt agcaggtgaa aataaatcac cagtaatgat tgtagaaaaa
      601 ttactttatg aatctcaaaa aaacttagtt aagagtaaga gatttgataa gataactaaa
      661 gttggggaaa tgtgtttgat gagataaaaa aattagtaaa atagtataat ctaaataaga
      721 atattttgtt gattaaacta cactaatagt aatattgtag ggggaaaaga tgagagaaga
      781 atcgaactct ttaagtttag accatataga aattttcttt gaacttttat ctcaaagtac
      841 agaagattat atattttttt gggatataaa taggaataaa tttaaaattt cttcagcaat
      901 ttttgatgaa tttaacttaa gtaaagagat agaatctgat ttagtcaatt gttggagtaa
      961 aattgtttat ccggatgatg ttcaaatatg gaaagatgat atacaagaat tactacatgg
     1021 aaaaaaaggt gagcacaatt tagaatatag gttgattaat aaatatgaag aaatagtatg
     1081 gatttcttgt agaggtaaag tttatgtgtc ggatgaccca aaaactatat ttctagtcgg
     1141 gagaataaag aatattggtg aaaaaaataa atttgattct atcacaggaa cgtggaatag
     1201 agaacaattt gaacatagaa tgaattatct tataaaagag aaaatttata aaaatggtgc
     1261 tatgtttatc atggatattg ataattttaa aaatataaat gaaaaatatg gacattctta
     1321 tggtgacaaa gtattgcgtg caattgctac tgaagtatta gaatatcttc caaaagatgt
     1381 aagattgtat aggttagatg gagatgaatt tgcatttttt taccctatgt gtactaaaga
     1441 aactatagag aaaatatatg aaaagataca gatgtataca aatactcagc atgaaattga
     1501 atcaaataag tactattgca cagtgactgc aggagttgct atgtatccag aagatggaga
     1561 taattattta gatttattta aacatgctga tattgcattg gatatagcaa aaattagtgg
     1621 taaaaataga attaagtttt tttcacaaga gctctatgaa aataaactaa aggtaatttc
     1681 aatgcaacaa aaactaagag agtgcgttga aaataatttt aatgactttg agctgttttt
     1741 tcaaccccag gtaaatgctg ttacaaaaga ggtaataggg gcagaggtac ttttaagatg
     1801 gcattcgagt acttatggag aagtatctcc tgttgaattt ataccaatac tagaacagag
     1861 taatttaatt ataccagttg ggaagtggat tataaaagaa gctgtaaaac agtgtaaaga
     1921 atggcataaa ataaatccag actttaaaat atctgtaaat gtatcgtata ttcagttaaa
     1981 agaagatttt tttagagatt ttatagtaga atgtttagta gagtatcaat taagacctga
     2041 atttttaatt ttggaattaa cagagaattg ttggatacca gatataaatt tactaaatga
     2101 taaatttatt agtttaaagg gtataggagt atatattgca atagatgact ttggtacagg
     2161 gtattcatct ttaaattacc ttaaagaact gtctgttaat ataataaaaa ttgaaagaag
     2221 ttttgttaag aatattacat ataatagtta tgaatatact tttcttgaat atattattaa
     2281 attagctcat attataaatt taaaagtttg tgttgaggga atagagtctt atgaagagta
     2341 tgatatagta aaaagcttag gtgtagatat tattcagggt tttttatttg gaagacctgt
     2401 aagtgcctct gaattttata aatttcaact atcaaagtga tgtacataaa atgagatata
     2461 tagtatataa aaatagggca aagtggaagt tttgaagttt attaaaaatt tacactttac
     2521 cctattttgt tatgtcttct ataaaggatt aatatactac actaactatt atatactttc
     2581 actccaaatt tagagattat ttcactagct ttttggacat cttccttagt ggcatcagga
     2641 acatctttta gttcataatc gatacctaaa ctttcccatt tgtgtattcc catagaatgg
     2701 tatggtagca attcaaatct ttctacattt ttcaatgtag ctacaaattg acctagtttt
     2761 tctaaattat ctatatcatc agtaatgcca ggaactaaaa catgtcttat ccatacagga
     2821 atatttcttg attctaagtg tctagcaagt tttaatgcct tatccatacc aacaccagtc
     2881 aaatcaatag atttttcctc aaccatatgt tttaaatcaa gtaaaaccaa atcagtatta
     2941 tctaaaactg ggtctatagt ttctatatct acgaatccag atgtatccaa gcaagtgtgt
     3001 atatcatttt cttttgcttt tttaaataat tcacttaaaa attctggttg aagagtggat
     3061 tcaccaccag aggcagttat acctccacca gaaaacttca taaaagaagt atacttaaga
     3121 gcatccgtta ttatttcatc aactgtgtat tcttttcctg atttaatatc ccatgtatcc
     3181 ctgttatgac agtatttgca tctaagagga cacccctgaa aaaataatat atatcttatt
     3241 cctggtccat ctacagtacc aaacgtttct atagaatgta tctttccctt aatcatagag
     3301 ttcacctcac aatatatttt aataacctta tcaatagtta taatatttgt ctaaaaaagg
     3361 ccatcttata ccagaaataa ttcaaatata agatagcctt ttatttttat tttttacaca
     3421 gtagtaatct tagttaaagt ataaaaattt aaagactaag ccattttacc gtggaatgtt
     3481 ctgtttataa catctaattg ttgctcttta gttaatttta tgaagttaac tgcatatcca
     3541 gatactctta tagttaattg tggatattct tctggatgtt ccatagcatc ttctaaagta
     3601 gctctatcaa atacatttac atttaagtga tgagcatttt gagagaagta tccatccatc
     3661 attgcagaaa gattgtttat tctttcagtc atatcttttc ctaaagcagc aggtactata
     3721 gagaaagtat tagatatacc atcttgagca tgttcatatg gtaatttagc aactgaagat
     3781 aatgaagcta aagcaccatt agtatctcta ccatgcattg ggttagcacc aggagcaaat
     3841 ggagcaccag ctcttctacc atctggtgta ttaccagttt tcttaccata aacaacattt
     3901 gaagttattg taagtataga ttgagttgga taagaatttc tataagtttt attttttctt
     3961 attttattca taaagctttc aactaactct acagctatac tatcaactct atcatcattg
     4021 tttccgtatt ttggataatc tccttctatt tcaaaatcaa cagctattcc ttgttcattt
     4081 ctaatagttt taaccttagc atgttttata gcagataatg agtctgcaca aactgataac
     4141 ccagccatac cacaagccat agttctaaat atatctctat catgtaatgc catttctaat
     4201 gcttcataag aatatttatc atgcatataa tgtattacat ttaaagtatt tacatataat
     4261 gttgctaacc aatcagtgaa tggttcaaat ttactcataa cttcatcata atctaagtac
     4321 tcagaagtta taggttcaaa tcttggtcca acttgaacac cagatttctc atctacacca
     4381 ccatttatag cgtataataa agttttagct aagttaactc tagctccaaa gaactgcatt
     4441 tgtttaccaa ttctcattgc agatacacaa caagctatac catagtcatc tccccagtat
     4501 gctctcatta aatcatcatt ttcatactgt actgaacttg tatctataga tactttagaa
     4561 cagaaatctt tgaatccttg aggtaattta gttgaccata gaactgttaa gtttggctct
     4621 ggagaaggtc ctaatgtata tagagtattt aacattctaa atgagttttt agtaactaat
     4681 gttctaccat ctattcccat acctcctata cactcagtta cccaagttgg gtctccagag
     4741 aataggttat tgtaatcagg agttcttagg aacttaacca ttcttaattt cataacaaaa
     4801 tggtccatat actcttgtaa ttcttcttct gttacgattc cagcttttaa atctctttca
     4861 aagtatatat ctaagaaagt agatgttcta cctaaagaca ttgcagcacc attttggtct
     4921 ttaacagcac ctaaataacc aaagtataac cattgtacag cttcttttga gttagttgct
     4981 ggttttgata tatcaaatcc ataagattca gccatttttt taagttcatt taaagctgat
     5041 atttgctcag ttatttcttc tcttaatctg ataacttcct catccatgca agaaacttct
     5101 aatgatttct tttgctcatt tttatcttct attaaagcat caacaccgta tagagcaact
     5161 cttctgtagt caccaattat tctacctcta ccataagcat ctggaagacc tgttattata
     5221 ccagattttc tagcagctct catttctgga gtataaacat caaaaactcc ttggttgtgt
     5281 gtttttctgt atttagtaaa tatatctttt atttctgggt ctaattcata tccaaaagct
     5341 tcgcatgaat tttcaaccat ctttatacca ccatatggca tgacagctct ttttaaaggg
     5401 gcatccgttt gtaacccaac tattgtttct aattctttat ctaaatatcc tggagcatat
     5461 gcagctattc cagaaacagt ttttgtatca acatctaaag taccaccatt ttctctttct
     5521 tttttaaata ggaccattgc ttcgtcccat aattttttag tattttcagt ggcaccagct
     5581 aagaaagagt catttccctc atatggagaa tagtttaatt gtataaactc tctaacattt
     5641 atttctttag tccatcttcc tgttttaaat ccttgccatg cattcattaa gtatttcctc
     5701 cttagattta aatgataaat aatactatta aaattatttt ttatatactt aatttatttt
     5761 atatattttt ttaaaaatat attttctaat ataataatat catattattc gtatcctgta
     5821 tacataaaaa ataaaaaatt gtcgctttaa agtataaaac agattatatg ttaataatgt
     5881 ggtgaaatta tacccatata taaataaaat aaaacaaata aatagctaat taaggagaaa
     5941 atatttttta ttttttgttt aggataaata aattttatta tggaaaaaag aaaaagcata
     6001 catcttcaca aattgaatat ttatttaata gggggaggtg atagattgtt tatcacagtg
     6061 gtattatttt tagtaggttt cctattaatt actaaaggcg cagatatctt tataaattgc
     6121 actgtagaga taggtagaaa aacgaatata tcagaaatta tacttggagc tactatagtt
     6181 agttttgcaa caacattacc tgaatttaca gtgtctctac ttgcttctat tgatggtcat
     6241 actactatga gtttaggaaa tgcagttggt tctataatat gtaatacagg attagcatta
     6301 gggttggttg tttttataag tccatttaat gtagataaaa aaatgttttt ttctaagtct
     6361 ttactattaa tagtatccgt tatagtttta attttattga gtttagatgg agttataaca
     6421 agaggtgatt cattattact tataataata ttaatctttt atatggttaa taattataga
     6481 agtgtcgttg gaaaatctga tactaaaaat agaaatatta aaaataacac tattaaagac
     6541 atatctacta aaaaagggaa aaattataga ggtttttccg tattagaaat tgtaaaaata
     6601 ctattacttt ttgctacagg attgataatg atgataatag gttcacaaat ccttatagag
     6661 agtggagtaa taatagctag ttttttaaat atacctcagg gtatagtaag tttaactata
     6721 atagcacttg gaacttctct gccagagata gtttcgtcga ttactgctat aagaaaaaat
     6781 catcatgaaa tatcagtagg aaatattttg ggtgcaaaca tattgaatat agtatcagta
     6841 atagctgtat cagcaatacc aaataacata ccaatactat ctcaaaatag acaattggat
     6901 ataccattca tgatattatt attattaata gtaataatac caacacttaa atctaataga
     6961 cttagtagaa tacaaggtat attgatgtta ttcacatatt ttctgtacat atcaattttg
     7021 tatttcatgt atattattta actatactgt ttaaaatatt aattgcagaa tatcctaaaa
     7081 aagtatataa tggataggtt aagattaatt gataacggag aggaataata tatgaatata
     7141 acaaagtttg aagatttacc aataagtgag ggaataaaaa aagctatagc tgaaatgggg
     7201 tttgaagaac catctcctat acaagcacag tctatacctg cgattttgtc aggtaaagac
     7261 gttataggac aagctcaaac tggtacggga aaaacagctg cttttagtat ccctatacta
     7321 gaaacaatag acccaaataa tagaagttta caagctgtag tgctttgtcc aacaagagaa
     7381 ctggcaatac aagtatcaac agaaataaga aagttagcta agtattcgca cggaataaag
     7441 acattaccta tatatggtgg ccagccaata gatagacaaa taaaatctct aaagagtggt
     7501 gtgcaagttg taataggtac accaggacgt acaatagacc atatcaatcg taaaacatta
     7561 aagatggata atgtaaaaat gattatatta gatgaagcag atgaaatgct agatatggga
     7621 tttagagaag atatagaaat gattttaagt aagattccag aagaaagaca aacaactttc
     7681 ttttcagcta ctatgccaag aggaattttg gaattaacta agagatatca aaaagaccca
     7741 gagcatataa aagttgttag aaaagaactt acagtttcaa atacaaagca atactatata
     7801 gaaactaggt catcaaataa attagaagta ttatgtagat tagtagatgt atatgaccct
     7861 aaactatctg ttgttttctg taatactaag agaaaagcag atgaattggt aggagattta
     7921 caagcaagag gatattttgc agatgcatta catggtgact taaaacagac tcaaagagat
     7981 atagtaatgg ataagtttag aaatggtaca atagatatac ttgttgctac tgatgttgca
     8041 gctagaggta tagatgttga tgatgttgaa tgtgtattca actatgactt accacaagat
     8101 gaagagtatt acgttcatag aattggtaga actggtcgtg ctggtagaga aggtatgtca
     8161 tttacttttg tatttggaaa agaaatgaga aagatgaaag atatagaaag atatactaaa
     8221 tctaaactaa taaagcataa tataccaaca ataactgatg ttgaagagaa aaaagtaggt
     8281 actttctttg cacaagttaa acaaactata gaagaaggtc acttaactaa acaattacaa
     8341 tggttagaag gtttctgtaa tgatgaagat tatgcaatgg ttgatatagc tgctgcactt
     8401 gtaaaactat ctctaggtga agaaatgaaa gaagaaataa tagaagaaaa acctagaaga
     8461 gagcgtggag atagaaaagg tggtactgga gctaaagatg gtatgattag attatttatc
     8521 aatattggta gaaatcaaag agtacaagca aaagatatag ttggtgctat agctggagaa
     8581 gttggaatac caggtaaagt agtaggtact atagatatat atgataagta tacttttgtt
     8641 gaaataccta aaaaagatgc aaaaacagta attgaaaaga tgaaagatat taagataaaa
     8701 ggtaataaga ttaatataga aaaagcaaat aagaagaaaa aataacgtat agtatacaaa
     8761 actataatta gaaaatatat aaaataatat attggatatt taaaaagcct gatgtgtttt
     8821 caaaacagat agtagataat ctgtaagagt taacagcata aggcttttct tgttttatat
     8881 gatattatga ttttccagat cgttgtgaaa aatgtatatt agttcctttt tttcaaatct
     8941 gatttgaaaa agagaatatt aattatctat atagtttaga ttataataaa aaatgtaaga
     9001 taacaaaggg gagaaaaaga tgtcaatgac ttatatggac cctagggttt ttttgatttt
     9061 agaaatggtg ttagaagaag acacaaatac tctagaacaa ttttcaaatg ttttgggtgt
     9121 tagtactagg actatcagaa actatataaa ccagattaat cgagaattaa aaggtggtat
     9181 tgctaaaata gttaaaaatg aaaaaggtac atatagtata agaattgagg atgaaagtaa
     9241 attaattgag attgtaaatt ttaatagagg aaagctatca aattttataa atttaaattc
     9301 acctgatgaa agaataaatt atgttcttga tattttaact atgaccaaca atccaattac
     9361 catagatgat ttggctgaag agatatgtat aggaagaaca acattaataa aggatttaaa
     9421 aaaagcagac agaattttag ctgaatatga tttagaatta aaaaggaagc caaatatagg
     9481 tatgatttta gttggtgaag aaatggatat aagattatta atccttgata gattgtatga
     9541 aaattatatt gatgtcttgg aaaacgttaa tcagataaat ttagttaaag attatgatat
     9601 tgaatgtctt agagatgaat taaaaaagtt gtttaaaaaa gaagaactct acataacaga
     9661 acaggtcttg agagatgttg aaagatatat aatcatgtct gtcttaagaa atctaaatgg
     9721 gtacaaattt gaaaagatag ataagagatt tgaagtgata agatgttcag atgaatatac
     9781 tttaggatta aagctcaaag ctttattgga aaagatattt aatatagttt taaatgaaaa
     9841 agaaaccata tttttgacaa tgccattaat aggtagaaaa gctcctagta caaaattagc
     9901 acttagcagt ataaagataa atgatagcgt aaaggaagtt gtagatgaag taactagttt
     9961 tttattagag acctcaggga ttgattttaa agaagataaa aagcttatag aaaatttgga
    10021 atatcacttg tattttgcac ttaatagaat gagattcaac ataagggtaa aaaatcctct
    10081 tttacaagaa ataaaggaaa gatatacatt tccatatagt gttgcaaaga tagctgcaat
    10141 gataattgag gaaaagtttg acttgaaaat atgtgaccac gaaataggat atatagcatt
    10201 acattttgga agttattttg aaaaaaattc aagaaaagtt ctaaatgtaa atagggtagc
    10261 tgttgtttgt ggaacaggtc ttgggactgc acaacttatt agaataaaga taggaaaaat
    10321 acttggagaa agtgtagaag taaatacttt ttctgatata gaagctaaag ctaatttaga
    10381 tttattaaag tattatgatt tagtatttac aacagtagat ttcaaaacag acttagatgt
    10441 tccaattttt agagtaaatg cgttatttaa tgaagaaagt ataaagaaag agatagaaaa
    10501 tgcaatactt ttgaaaaata gtataaatct taagtctata aaaaagattg aaaaaaatat
    10561 cccttttata ggtaaattaa ttgaagagga taagttcttt atattgaata agagtacgtt
    10621 tatggaaaat ttagaagata tgttagaaaa attagttcat aaaggaatta ttgatataga
    10681 gttcaaaaaa aaggtattaa aaagagaaaa gaaatcttca acagcattag ataattatat
    10741 agcattgcct cactcagtta atactaatgg ggaaaatctt tatctgtcat ttggaatatt
    10801 agaaaaacca gtggtttggg atacaaagga aattagaata ataatattga tgataattcc
    10861 agataagaat gtagatagta cagacctgat aataaaaagt tatgaagaag tacttgaagt
    10921 agggagagat aaaaaaatga tagaagaact tgctaaagta aaatcatttg atgaatttta
    10981 taaaataatt accaagagga ggtaataata tggacccagt aatttttatt atagcatcac
    11041 ttattacagt gtatttatta aatatttttc ttggatactt acagcttaaa gattttaata
    11101 aaaattatat tgaactaaaa agaaaaggaa gagtggcaat aggcagaaaa aaaggaagaa
    11161 tttcatcagg tacaattgtg ttgatactta taaatgatga tggtgtgatt gtcgaaacta
    11221 gaaaaatgca aggtgttaca gtattggcaa gagtaaagtc atttgaaggt ttggtaggaa
    11281 agagcttaga gtacatagaa aaatcagatt taactgttta caataaacta cttaaaaaag
    11341 caatactaga tgcagtcaaa cagtacataa catttgagaa aaataaatgt gaagatacaa
    11401 ataatctgag tactgatgaa gagtgtagga tggaagtagt ttaaaaaaat atatttataa
    11461 ggaggagtta atatgacgca attttttcaa gtattagcaa gtggtgcaga tagttttatg
    11521 aatttattta gggcaggtgg gcaacaattt gtaggttttg taacagatat agtaccactt
    11581 ttagtagcat tattagttac aatgaatgca attatcaact ttattggtac agatagagtc
    11641 gagagacttg caaaaaaatg ttctgcaaat atatttacta gatatttgat attaccagtc
    11701 cttggtacat ttgtatttgc aaacccaatg acactatcac taggaagatt cttgccagaa
    11761 aagtttaaac ctagttactt tgcagcagca agcttttcat gccatacaat gaatggatta
    11821 ttcccacata tcaatccagg tgaactattt atatatctag gaattgcaaa tggtataaca
    11881 actttgggat tctcaacagc agatttagca gttagatatt tgttagtggg tatagttgca
    11941 aactttatta aaggtgtagt tactgactat acaacaaaat ttgtagagaa acaacaagga
    12001 atagcattaa attcagatat aaagattgga taattacttt aaggtataaa taaattttta
    12061 agtttaaaaa cacaataaaa atctgattat ccgctaaaac acagtatcta tcagcatttt
    12121 caagaaaaca aatacatcaa tttactactt atttactact aacgaatgag ccttgatacg
    12181 caagttttcc tagatatgca aagatatggt acagcacatc agtattaaaa taagaaaaaa
    12241 ttgaataact catagactat ggaacgccta acatgacgtt ccttttctat ttccagccgt
    12301 tcttattgga cggctatttt attacccaaa atgaaaggag cattagattt atgaaaaaga
    12361 tgttaaaacg attgtgtacg ggcttcttag ctcttgcaac tgtcgttact gctttaccga
    12421 ctacacccgt ccatgcagaa agtaagcaat actggacgga aagtgcagag cgtgtcggta
    12481 tcattgaaaa agtaatgaat gacggttcta tcggttcgac attcaatgag ggctatatga
    12541 aagtcgaggg cgaaactgcc tattgtatcg acattaacac aaactttaag aacggttaca
    12601 agaccagagc tgacgcaagc tcacgcatga gtgctgacca gatttcagat gtggcgttat
    12661 ccttagagta tgtcaaacag tatggcgaag ctcacaagga actgaactac aaacaagtct
    12721 atctgttgga acaatgtgta gtctggcaga gattgagcgt acattttggc tggcaatgtg
    12781 ataacgtgcg agcttcttat gatgaaattc caaaggcaac acaggacgaa gttttctctg
    12841 gtgcaaaagc ctttatcaaa gaaaataaag gacgctatga atgtggcggt tatatctact
    12901 ctggcgaggg gcaggaatta ggacaattct gggcgaagct gaatgtcgga aatgcgaaac
    12961 tgcaaaagac ttccagtaat accagcatta ccgacggtaa cgggaattac tctattgcag
    13021 gtgcaaccta tggtgtcttt tctgataagg actgtacgaa acagcttgcc acccttacga
    13081 ctgatgaaaa cggaaataca gatgttgtag aggtaaaggc tggcacagtt tatatcaagg
    13141 agttatccgc accagcagga tataaagtag ataaaaccat atattcctta aaggttgaag
    13201 ttggaaagac agcaattttg aaagtatcag atacgccaaa agtaacggac actttgattg
    13261 agcttttcaa gattgatatg gaaacacaga aagacaatcc gcaagggaac gcttctttag
    13321 caggtgcgga atttacatgg aagtattatg caggcttcta taataaagac aatctccctg
    13381 ccgaagctac tcgtacatgg gttacaaaga caatcgctga aacagacagc gacgggacaa
    13441 ctcactacat cgcaaaatta gcggacgcat acaaggtatc tggcgatagc ttctatatgc
    13501 aggacggcaa ggcggttctt ccacttggaa cgctaaccgt tgaggaaacg aaagctccaa
    13561 acggctactt gttagacggt gcatatatgc aggctggcga taagtccgaa cagataaaag
    13621 gcttatatgt aacacagatt acagaggacg gcgaccttgc cgtacttact ggaagtaacc
    13681 agttttctgt atcagacaag gttatccgtg gtggtgtgaa aattcagaaa cgagatttag
    13741 aaacgggcga tacaaagcca caaggaagtg ctaccttgaa agatactgct tttgacatca
    13801 tttccttaaa tgataatgcg gtattggttg agggcaaatt atacaagaaa aatgaagtgg
    13861 taaaaacaat ccataccgat attgagggtg tcgcttctac ttcttctgac cttttacctt
    13921 atggaaaatt ccgtatcgtt gaaagtgaag ctcccgacgg ttacttaact gacggtgcaa
    13981 agccgattga ttttacaatc acagaaaatg gaaaaatcgt ggacttaacc gacgaagctc
    14041 attctatcta taatcagatt aagcgtggag atattgaggg tgtaaaaatc ggtgcaggta
    14101 cacacaagcg tcttgctaat gttcccttta ggatcacaag caagatgacg ggcgaaaatc
    14161 atgtcgtggt aactgatgat aacgggcaat tctccacttc tgctgaatgg gcttctcata
    14221 agcacaatac aaacgcagga aagaccagcg aggacggtgt gtggtttgga acttctgaac
    14281 cagatgacag caaaggtgcg ttaccttatg atacctatat cattgaagaa ttacgctctg
    14341 atagtaacaa aggttttgaa cttatcccac cttttgaaat cgtggtatca agaaataacc
    14401 ttgtgattga tttaggaacg ctgactgatg aatacgaaaa agaaatctct atccatacca
    14461 cagcgaccag caaagacggc gaaaagacta tccttgcagg aaaagagatt acgattgttg
    14521 atactgtcaa attagacgga cttacaaaag gcacaaagta tcagttaaaa ggctggcaga
    14581 tgttaaaaga agaaaatgta gaacttctta tcaatggaaa acgtgtggaa aatgattata
    14641 cctttgtcgc tgatgatgaa gaaatgaaag tggaaatttc ctatacattt aatgcgtctg
    14701 ctttaggtgg caaaaacctt gttacctttg aagaattgta tgatttaagc aatccagacg
    14761 aacccgtaaa agttgcggaa cataaagaca ttgaggacga cggacagacg gtacttatca
    14821 cagagcgtat cgtcaaaatt catacgactg ctacggataa ggacggcaac aaagagcttg
    14881 aagctggaaa agacgttaca atcattgata ccgtaatctt agagggctta gaagtcggta
    14941 cacagtacaa acttgtgggc tggcagatgt tgaaagaaga aaatgcagag cttcttatca
    15001 atggaaaacg tgtgaaaagt gattacactt ttactgctga cagcgaaact atgaaagtgg
    15061 aagttgcctt tacctttgac gctacttctc ttgacggcaa acagcttgta acttttgaag
    15121 aattgtacga tttaagcaat ccagacgagc cgaagaaagt taccgagcat aaggatattg
    15181 aggataaggg acagacgatt acctttaagg aaaaaaccaa agtcccagaa gaaccaaaga
    15241 aacccgaaac accacagaca ccagatacac cgcataaaac agacagtcca aaaactggcg
    15301 atagcacaaa cctttacgga ctgcttgcac ttcttttgac ttctggtgct ggactggcag
    15361 gtatcttctt ctgcaaacgc cgtaaaatga agaaatcata aggcggtaac ggtatgagaa
    15421 aagaaaaatc acgttctccg ccgataccgt gtcggcagac ttccgcttat tctggattgt
    15481 ctgctactta cctgcaagaa cacggatagt caagggtgcg taagcattga ttttaccctt
    15541 tactatctgg gggattgctg gtacttccca aaccgccaga ataagaaatc acaatcaaaa
    15601 aacttatcaa aaatagaaag aaatgaggta aaacactatg gaaatgaaat atgtcgtgcc
    15661 agatatggca cagtcttttg gaactcttga atttgcaggc gaaagtgagc cagtctttga
    15721 aagagataaa aataaccaca gggtcttagc cagacgaagc tataaccttt attctgacgt
    15781 acaaaaaggc gaaaatgttg tggtagaaat tcccgtgcag gctggcgaaa agcatttcaa
    15841 gtatgaacag aaagtaaaac ttgtcaatcc gaagttatat ggcagaggtt acgcaatcgg
    15901 ggatatggga cataccgatt atgtgttgct tgctgatgat attgtagcag ttgaagaaaa
    15961 gtaaaggagt gaagaattta tgagattatc aaacgggttt gttattgaca aagagaaaac
    16021 atttggagaa ttaaaattta cagcggtgcg agatgtgttc ttgcagaatg aggacgggac
    16081 accgagtacc cagcttaaaa agcgtatcta tgatttgaag tgcagtctgc atggtggaat
    16141 tatccccgtg tccgtaccgc cagaagtacc gttaagggaa tttccgtaca atgcggttgt
    16201 ggagcttgtc aatccagtag ccgatacggt atcaagaaaa acctatacgg gtgcagatgt
    16261 ggactggtat gtaaaggcag aggatattgt attgaagaat aaaagcaacc agaacgcagg
    16321 caatacacag aaccatacac cgcagggaca accgaaaaaa tagacttgct gaatagattt
    16381 tcgttgtaga ggatatacgg aactgttata atatgggtat gaagaaagcg acaaattaaa
    16441 atttttaggg agtgagaata gtatatggac agttggttta ccgtcgaaca aatagaccaa
    16501 gatacattcg tcatcagcga atacaaacac tgggaagaaa cacattgtta tctattgtgt
    16561 gggacaaaaa gagccattct gattgataca ggtttaggtg tttccaacat cagagaggtt
    16621 attgatggat tgacgaaact gcccgttaca gtaatcacta ctcatgttca ttgggatcat
    16681 atcggtggac ataaatattt tcggtctatt gcagttcatg aagcagaaaa agaatggcta
    16741 tcggtcaaat tccctatacc tttgcaagtg gtaaaaaaca atattatgtg caatccatgt
    16801 gatttcccct cggattttaa ggtagagaaa tatcagattt ttcaaggggt tccgcaaatg
    16861 attttacatg atgaagattg tattgactta gggaacagaa agttggtagt tattcacacg
    16921 cccggtcatt ctcccggaca ttgttgtttc tatgaagcgg atagaaaata tctgtattcc
    16981 ggcgatttga tatatagcgg ttgccttgat gctttttatc catcaacaaa cccacaagaa
    17041 ttttggaagt ctgttagaaa aattcaatct ttaaagataa gtcgaatttt gccaggacat
    17101 catcatttat ctattccagt tactataatt gacaagatag agacagcttt tcacaacttg
    17161 tcaaatgaag ggaaattaaa acaagggaat ggtatattca gctatgaggg ggttcaaatc
    17221 catatctgaa atgcaatctt agttttacaa ttttatatcc ctacacacaa agcagttagt
    17281 cttatgattg actgcttttt tcatacccag aaaggagtga ttgatttatg cgtatgattt
    17341 ggaacaaagg acaccgtatc agagccagcg acaagcacct tgtctatcat ttttccatag
    17401 agacgcttct gtttgtgttc gtggcagttc tcctgctact gaatagcaaa cagctcatgc
    17461 gtaccgactg ggaacatttc agcttattag acaatggctt tacgctttcc ctttacaact
    17521 tcataaccat actgatagcg actggtgttt gtgcattggt cgcttttctg tattaccgct
    17581 tctgttacga cagtttcaag aagctactgc accgtcaaaa gctggcaaga atgatactgg
    17641 aaaataagtg gtatgaagcc gataccgtac aagacagcgg tttttttact gacctgcaaa
    17701 gcagatcaag ggaaaaaatc gtctggtttc caaagattta ttatcaaatg gaaaaaggac
    17761 ttcttcatat ccgctgtgaa attacgctgg gaaaatatca agaccagctt ttacggttgg
    17821 aggataagtt ggaaagtggc ttgtattgtg agctgaccga caagaccctg catgacggct
    17881 atatcgaata taccctgctt tatgatatga tagcgaaccg cattaccatt gatgaagtac
    17941 gggcagaaaa cggttgtctt agattgatga aaaatcttgt ctgggaatat gacgcactcc
    18001 ctcacgctct gattgctggt gggactggtg gcggtaaaac atattttctg ctgacactca
    18061 ttgaagcctt actgcataca aacgctgtcc tttatatctt agacccgaaa aatgcggact
    18121 tagcagactt ggggacagtt atgggaaatg tgtatcacac gaaagaagaa atgattgatt
    18181 gcgtcaatgc cttttatgag ggcatggtac agcgaagtga gaaaatgaag cgacacccga
    18241 actataagac gggcgaaaac tacgcctatc tgggactgcc accctgcttt cttatctttg
    18301 atgaatatgt ggcgtttttt gaaatgctgg ggacaaaaga aagtgtgagc ctacttagcc
    18361 agttaaagaa aatcgttatg ttaggacgac aagcaggtta tttccttatc gttgcctgcc
    18421 agcgtccaga tgcaaagtat ttctcggacg gtatcagaga taatttcaat ttccgtgtag
    18481 gacttgggcg tatcagcgaa ttaggttacg gtatgctgtt cggttctgat gtgaagaaac
    18541 agtttttcca gaaacgtatc aaggggcgtg gctattgtga tgtgggaaca agcgtcatca
    18601 gtgaatttta cacgccttta gtcccgaaag gacatgattt tttacagact atcggttctc
    18661 ttgcacaagc aaggcaggac gggacggcga cgtgcgaagc gaaaggcgac ggcacggact
    18721 agccgttgct ggtgtggcgt aagccacgcc agcaggtaaa acccctcggt atctaacaga
    18781 ggggtacgtt tgcaaataga caatagacga aaaacacagg aaacataagg cttatcgcat
    18841 ggtttcctag caaaaatcgg ctgtgaagtc gccttaaaaa acttcacact tgacagattg
    18901 ggggtatggt tctgaatgaa gaacaatgga taaaagaatt acgggaaaaa cggattgctt
    18961 acggtatctc acaaggcagg ctggcggtgg cgtctggtat cacaagagaa tatctcaaca
    19021 agatagaaag cggaaaaatg aagccgtcaa aggaacttct gaatactctg cataaagaac
    19081 tggcaaagtt caatccagaa gcaccgctta ccatgctgtt tgattatgtg aaaattcgtt
    19141 ttcccacgct ggatatacag cacatcatca aagatatatt aaaactgaat atcaactata
    19201 tgctccatga aaattacggg cgttacagtt atacggagca ttactcttta ggggacatct
    19261 ttatctatac gtcggctgac gaagaaaaag gtgtcctttt agagttaaag gggcgtggtt
    19321 gcaggcagtt tgaaagttac ctgctggcac agcaaagaag ctggtatgac tttcttatgg
    19381 acgcacttat agacggtggc gtgatgaagc gtatcgacct tgctatcaac gaccatacgg
    19441 gcattttgga tattccagag cttgcggaaa aatgcaggaa acgggaatat atcggaaagt
    19501 ccagaagcta taagttttac cagtcgggcg agcttatcaa gcacagagag gacgacagag
    19561 aatatatggg gcgtaccctt tatcttgggt cgctgaaatc agatgtgtat ttctgtatct
    19621 atgaaaagga ctatgagcag tacgtcaagt tagggacacc tctggaagaa gccgacatta
    19681 tcaaccgttt tgaaatacgg cttcgcaatg aacgagccta ttatgcggta cgagatttac
    19741 tgacctatta tgacgcagaa cagaccgctt tttctatcat caatcagtat gtgcggtttg
    19801 ttgatgaaga acccgacaag cgaaaaaatg actggaagct caatgaccgc tgggcttggt
    19861 ttatcggcga taacagacag agcttgaagc tgacgacaaa accagagcct tacaccttag
    19921 accgcacatt gcggtgggta caacggcagg tagcaccgac cttaaaaatg ctgaaaaaga
    19981 ttgataaggg aaacggtaca gactacatgg aaacaatcga acagcaggca aagctcacag
    20041 aaaagcatga aatgataatc aaacagcaga cgacccctgc aaaagattta gtagaaagtt
    20101 aggagtgata aaaaatgtgg gtattattaa aagacttcct gctggtatct atgggaatgg
    20161 gtatcggcgt agtcttgatg tgtattttga atgtcggcaa agaagctgac tatgaaatgg
    20221 aacaattaaa agaaagcgag gacaattaaa tgaatttcgg acaaaattta tatcaatggt
    20281 ttttatcaaa cgcacagagc ttagtgctta tggcgattgt ggttatcggt atctacttag
    20341 ggttcaagcg tgagttttca aagctcattg gcttcttggt agttgccttg attgctgtcg
    20401 gcttggtatt caatgccggc ggtgtgaaag atgtactgtt agagctgttc aacaagatta
    20461 tcggtgcata aaattttatt gtagtgctaa tatgtgagtg atataatatg gatatgaaga
    20521 aaacgacaaa tatgaatttg aacttaggag gaaaactatt gtgtcaaata taaaactaag
    20581 aaatacatat aagtcttata cagcgatatt tgtaattggt atattagtag ggtgtatatg
    20641 tagactgtta gattattttc ccgcagatac actatggagt ttttcatcac ctcaaacatt
    20701 gtttggtttt tggattatta ccaatactat catcgttatg ttaagcacct caaatatttg
    20761 tgcaggaatt agttcgttct tgtatatgtt tggaatgaca ttaagttttt atgctttaca
    20821 agcaatatta ggaatgttta tacctatgtt ttccggcgga ttccgctttg ggctattcat
    20881 attgtttact gtttggtcaa tcgcttgtgc aatagcagca tttattctgt actattggaa
    20941 tagggaacat atttttagtt cagtattata ttctttacca gtaggagcat tatttgctga
    21001 aacaatagca gtttttattt attttttgga acatcaaaca ttcctatttc agttgttaat
    21061 ggatattata ggagctgttg tgtttaccat aatatttttc aaaaaagtaa actatagaaa
    21121 aatgtatatt gtcggaattg tattaagtac tttggtattt tattatattt ttccatggta
    21181 gtttatattg ataaatccca gtttgacaat ttcatatcca tacacacaaa gcagttagtc
    21241 ttaggattga ctgctttttt cttacctaaa ttttagattg gagtgatgaa atgagcgata
    21301 tttttaagga tatgcagacg aaagtcggct gtgattatat ttccgacctg ccctcttaca
    21361 agcgtaaggt gtggcaggaa atgaaacgac tgaaccctgc cgattatgaa gaaagacagt
    21421 tagaagattt ttctaaatat gtgtttggta tgtcgtacca gaccttacaa gatgtgatga
    21481 aacaacagaa aggacgtgag gaacaatgca ggaaacaagg gtgctggtgg aaacgaaagg
    21541 aacaactggc gaagaaacaa catcatactg gtttgacctg ccgatagatg ttgcagagtt
    21601 tgaagaaaag ttaggtgtcg gtgcagaaag tggggattac cgcattatcg aaaaggtact
    21661 gccctttgct gatgaagtcc acgaacatac aagcgtgtac cagctcaacg aattagattt
    21721 tatgtaccgc cagctttcaa gcgatatgca ggaagaatat gtatcacttc ttaccgtgta
    21781 tgaaaattta gaagcacttt atatttgcag gaacgtgatt acggtttatc ccgactgcaa
    21841 aagcatgata gatgttgcaa ggcaaaagct gatgaacgac ccgacattta agcatttatc
    21901 cgaggactgt caagaatatt actttgactt tgaagcctac gcttctcact tgcaggaaca
    21961 cgggaaattt ttagtaacag aacacggtat ctttgaactg ccagagtagg aaatgaggtg
    22021 gtgcttatga ttgatgatat ggcggtttac attgctaatc ttggcaaata caatgagggc
    22081 tatttagtcg gtgcttggtt cacgttcccc attgacgagg aagatgtgaa agaaaaaatc
    22141 ggcttgaatg aacagtatga ggaatacgct atccatgata ccgacaactt ccccattgcg
    22201 attggcgagt atgtttccat tgaagaactc aatgagatgt atgaaatgat agaggaactt
    22261 cccgactata ttgtagagtg tctggacgaa tttatcagcc actacgggac gctggaagaa
    22321 gtcgtggaac acaaggacga tatttattat tatcccgact gtgaaactat gacagacgtt
    22381 gcctattact acatagacga attgcaggca cttggggaca ttccacccag cttacagaat
    22441 tacattgact atgaagccta cgggcgagat ttggatatgg gcggttgctt tattgaaaca
    22501 agccgaggta tgtgcgagat accatattaa cgctggaaga tcagcgacaa gcattgttgt
    22561 tactgacagc gtatttctct ttaagggaca gtctgaaaat ggctgtcctt ttctatttag
    22621 aaacttacga aaggagctga aagaacttga aaaagattaa atcttatacg ggtatctgga
    22681 acgtggaaaa agtcttgtat gcaatcaatg actttaactt accctttccc gttaccttta
    22741 cacagattac atggtttgtg attacggaat ttctcattat tctgtttggg gatattcccc
    22801 cactttccat gattgaggga gcatttctca aatactttgg tattcccgtt gctctcacat
    22861 ggtttatgtc gcagaaaacc tttgacggaa agaagccgta cagctttttg aaatcacaga
    22921 taacctatgc cctgcgaccg aaaatcactt atgcaggaaa agccgtaaaa ctgcataagc
    22981 agatactcaa tgaaacaatc acggcagtaa ggagtgtgaa ctatgttccc gataaaatat
    23041 attgacaata accttgtctg gaataaggac aatgaggtgt ttgcttatta tgagctgata
    23101 ccatacaact attctttcct atccgcagag cagaaattta tcgtgcatga cagtttccga
    23161 cagcttatcg cacagtcccg tgagggaaaa attcatgcgt tgcagattgc aacggaaagc
    23221 tcaatacgaa gtatgcagga gcagtcaaag aagctggtta cggggaaatt aaaggaagtt
    23281 gcctgccaga agatagacga acagaccgaa gcgttagtat caatgattgg ggacaatcaa
    23341 gtggactacc gcttttttct tggctttaag ctcatggtta cggaagaaca gctcaatctg
    23401 aagaacatca aaaaatcggc gtggctgaca ttcaaggaat ttctccatga agtgaaccac
    23461 acgctgatga atgactttgt ttccatgccg aatgatgaaa tcaaccgtta tatgaaaatg
    23521 gaaaagttgc tggaaaataa aatctcccgt cgctttaagg tgcgtcgctt ggaaatccat
    23581 gattttgggt atctcatgga acatctttac ggcagggacg gtatcgccta tgaagattat
    23641 gagtaccagc taccaaagaa gaaattacag aaagaaacgc tgataaaata ctacgacctt
    23701 atccgtccga caaggtgtgt gattgaggaa agccagcggt atttgcgatt ggaacatgag
    23761 gacagggaaa gctatgtgtc ctactttacc gtcaatgcga ttgttggcga gcttgatttt
    23821 ccgtcgtctg aaatcttcta tttccagcaa cagcaattca cattccccgt tgatacttct
    23881 atgaatgtag aaatcgtgga gaaccgtaaa gcattaacaa ccgtccgcaa caagaaaaag
    23941 gaattgaaag accttgacaa tcacgcttat caagcaggaa gtgaaacaag ctcaaatgtg
    24001 gtggacgcat tagacagcgt ggatgagctg gaaacggact tagaccagac aaaagaaagt
    24061 atgtataagc tctcttatgt agtgcgtgta tcggctgatg atttggacga attaaaacgc
    24121 cgttgtgatg aagtcaaaga tttttacgac gacctcaatg taaagctggt gcgtcctgct
    24181 ggggatatgc tggggcttca ttctgaattt cttcctgcca gcaagcgata tatcaatgac
    24241 tacgtgcagt atgtaaaatc agatttcttg gcaggtcttg gttttggtgc gacccagcag
    24301 ttaggggaaa ctacgggtat ctatatgggc tattccgttg atacgggaag aaatgtgtac
    24361 ctgcaaccgt cgttagcttc gcagggtgta aaaggtacag taacaaacgc tcttgcttct
    24421 gcttttgtcg gttcgcttgg cggtggaaag tcattctgca acaatcttct ggtatattat
    24481 gcggtgctgt ttggcggtca agcagtcatt ttagacccca aagctgagag gggcaactgg
    24541 aaagaaacgc tcccagaaat cgcccatgaa atcaatatcg taaaccttac cagcgacaag
    24601 gacaatgcag gacttcttga cccgttcgtg attatgaaga atgtaaaaga cgctgaaagt
    24661 ctggcaatcg acatcttaac attccttacg ggtatttcct ctagggacgg cgaaaaattc
    24721 cctgtacttc gtaaggcggt acgctccgtt acccagagcg acagtcgggg cttgctccat
    24781 gtgatagacg agctacgccg tgaagataca cccatatcaa gaaatatcgc agaacatatc
    24841 gacagcttca cggactacga ctttgcacac ctgctgtttt cagacggtac ggtggaaaat
    24901 gcaatcagtc tggataacca actcaatatc attcaagtag cggacttagt attaccagat
    24961 aaggatacaa catttgagga atacacgacc attgaattat tgtcggtgtc tatgctgatt
    25021 gtgattagta cctttgccct tgattttatc cattcagaca gaagcatttt taagattgta
    25081 gaccttgacg aagcgtgggc gttcttaaat gtggcacaag gcgaaacttt atcaaataag
    25141 ctggtgcgtg ctggacgagc tatgcaggca ggcgtctatt ttgttacaca atcttctggc
    25201 gacgtgtcaa aggaaagtct gaaaaacaat atcggcttga agtttgcctt tcgttctact
    25261 gacatcaacg agataaagca gaccttagaa tttttcggta tcgacaagga cgacgagaac
    25321 aaccagaaac gcttgcgtga tttggagaac ggacaatgct tattgcagga cttatacggg
    25381 cgtgtcggtg tggtgcagat acacccagtc tttgaagaac tgctacacgc ctttgatacc
    25441 agaccgcccg tacagagaaa tgaggtggag tgatgaaaga aaggataaaa ggtgcgttca
    25501 caaaaaggaa gatacttcac tttctcaaaa tggctctgtt tgtcgtggca ctctccctta
    25561 tcctgctctc acttctgggg acggtggctc atgcgacggg gcttgtggac gataccataa
    25621 acgcagaaaa tctttactca aaatatccgc tgtcgaatta ccagcttgat ttttatgtgg
    25681 ataatagctg gtcgtggttg ccatggaact ggctggacgg tatcggaaaa tcggtgcagt
    25741 acggacttta ctgcattacc aattttgtct ggacgataag cctttattta agcaatgcta
    25801 cgggctatgt ggtgcaggaa gcatacaaac ttgattttat taacgatatg gcagacagta
    25861 tcggacaaag catacagacc cttgcaggcg ttaccgagaa cggcttttct tctacgggct
    25921 tctatgtcgg tttcctgctt ctcattatct taatagtggg actttatgtt gcctatacgg
    25981 gacttatcaa acgcgaaacc agcaaggcac ttcacgctgt tattaacttt gtggtggtgt
    26041 tcgtgttgtc cgcttccttt attgcctacg ctcccgatta catcaagaag ataaatgaat
    26101 tttcatcaga catcagtacc gcttctttgg acttgggaac aaaaatcatg ctccccaact
    26161 ctgacagcga gggcaaggac agcgtggact tgatacggga cagcttgttt tccattcaag
    26221 tggaacagcc gtggctcttg cttcaatttg gtaacagcaa cgcagaggaa atcgggacag
    26281 accgtgtaga agctctggta tcagtaagtc cagaggacga ggacggcaag accagagagg
    26341 aagtcgtgaa aacagaaatc gaggacaatg acaacaacaa tctgacgata ccgcaggtcg
    26401 taaaccgttt aggtatggtg ttcttcctgc tattctttaa cttaggtatc acgatatttg
    26461 tattcttgct tacgggtatg atgttgttca gccagatact ctttattatc tttgcaatgt
    26521 ttttacctat cagctttcta ctttccatga taccgagcta tgaaagcatg gcaaagcagg
    26581 caatcgtgag ggtatttaat accataatga cacgggcagg aataacgctc attgtaacgg
    26641 tggcgttcag catttccagt atgttttata acatctccac agactacccg ttctttatgg
    26701 tggcgttctt gcagatagtg tgttttgctg gtatctatat gaaactgggc gacttaatga
    26761 gtatgttctc acttaatgct ggcgacagtc aaaacatggg acgaagaatt ttccgcagac
    26821 cgtatctgtt tatgcgacat agggctaggc gtatggaaca ccgtattgca agggcggtaa
    26881 gtgctggcgg tatttcgggt ggtgtggctg gtgcggtagc tggaagtgcc gttgctggca
    26941 agagagccga aagaaaaaat acggcttcta aagaaaatcg gggcaatacc acttccagca
    27001 tgggacagcg tgcaggctca aaggtgggtg ctgtcttaga tacgaaaaat aaagtgaaag
    27061 acaaggctaa cgctgtcaaa gagaatatca aggatatgcc gacacagacc gcttatgcgg
    27121 tgtattccgc aaaggaaaag gcaaagtcca gcgtatcaga cttcaagcgt ggcatggtgc
    27181 aggaacagca gtccagacag acgggacgct tggaaaagca ggaacagcat agacagaata
    27241 tcgctgacaa acgtatggag cttcaaaagg cacaagaagc aagacaaaga aagacggacg
    27301 gatcagcgac aacgggagct acccgtcccc atgagcgacc agccacagct tcaaccattc
    27361 caaagccgag tgcggaaaaa atgcaggaag tgaaacgtcc tgccacagcg accacttcaa
    27421 aagcaagtga accagtcaag acaaatgttg tgaaagagcg tccgttatct tctggtgctt
    27481 ctgataagaa agcgacccag cctgcacagc cagtacatag gcagaatgta gaaaaagtag
    27541 tatcgcagga aacacgccag aattacaaag cagagcgtac tacaaagaaa cagacctttg
    27601 aacaatcaaa gcgtacaacg gaacataccg aaaagaaccg taaccttgta acgaagaaag
    27661 gacagaagaa aaaatgaaac tgaaacatat cgctcttatt ggcagtctgt ttcctatcct
    27721 cttttccata gtccttttct ttggtgtcct cattagtgcg gacagcgacg acgagaacag
    27781 caacttttct tctggcatta cgggtatgaa tttatccgca gaagtcttga aacatcagcc
    27841 tatggtggaa aaatacgcca gagaaaacgg tatctccgag tatgtcaacg tgctactggc
    27901 tatcattcaa gtagaaagtg gcggtacggc agaagatgtt atgcagagtt cagaaagtct
    27961 tggtttaccg cccaactctt tagatacgga aagctcaatc aagcaggggt gtaagtattt
    28021 tgcgtccctg ctttcttcct gtaaaaatca aggtatcgac gatttgaatg tagcgataca
    28081 gtcctataac tatggcggtg gttatgtggg atatgtggca ggaaaaggaa agaaacacac
    28141 ctttaacctt gcagagagct tcgcccgtga gaaatcgggt ggaaagaaag taacctacac
    28201 caacccgata gccgttgcga agaacggggg ttggcggtat cagtatggca atcaatttta
    28261 cgtcgaatta gtcaatcagt atttaactgt tccgcaggta tcgggagagc tggcacaaaa
    28321 ggtcatgaat gaagcgttaa aatatcaagg ctggaaatat gtgtatggcg gtagcaatcc
    28381 caacacttct tttgactgtt cgggactgac gcaatggtgc tatggaaaag ctggtatctc
    28441 cttaccgaga accgcacaag cacagtatga cgcaacccaa catcttccac tttcgcaggc
    28501 aaaggctggg gacttggtat ttttccattc tacctataac gctggaagtt atgtaacaca
    28561 cgtcggtatt cttgtttcgc cgacacagat gtaccacgca ggcgacccga taggatatgc
    28621 agacctaagt agtagttact ggcaacagca cttaatcggt gcaggacgag taaaacaata
    28681 gaaagtgagg atttcatcat gtttagaaag aataagaaac agacagaaac tatcaaagaa
    28741 cccaaagaaa aaaaggtgcg tactttcaag gtaggcacac ataaaaaatc cgtgattgca
    28801 ttgtgggtgg tgcttatcgc aagcgtgagc tttggggcgt ataagaattt tacggctatc
    28861 gaccagcaca cgacccatga aaaagaaatc atagagcttc gcttgcagga caccaacggg
    28921 atagaaaatt ttgtgaagaa ctttgcaaag ccctactaca catggagtaa cagcaaagaa
    28981 gcgattgaag caaggacgca ggcaatcagc ggttatctga caaaggaatt gcaggacttg
    29041 aatgtggata ccattagaac cgatataccg accagctcca cagttacaga tgtgattgta
    29101 tggagtattg aacagtcggg aacggacact ttttctgcta cctacgaagt ggatcagcag
    29161 ataaaagagg gagaacagac aagcaatgtg aaagcaacct ataccgtaaa agtccatgta
    29221 gacgctgacg gggatatggt aatcgttcag aaccctaccc ttgcaccagc aatcgagaaa
    29281 tcagattatg agcctaagac accagaagca aatgcaagtg tagacgctga taccgtcaat
    29341 gacgctactg catttcttga aacattcttt aagctgtacc cgacagccac agaaaaagag
    29401 cttgcctact atgtatcggg aaatgtgctt gagcctatcg gcagggacta catttatgct
    29461 gaactggtaa accctatctt tacaaaggac ggggacaatg tgaaagtcaa agttgctgtg
    29521 aaattccttg ataatcagac aaaggctaca caaatatcgc agtatgaact tgtgctacat
    29581 aaggatagca actggaagat tgtaggataa taaaatatgg aaagtttgct tgacattctt
    29641 tacttgcagg agtatcataa gattagaaaa ggaaagcaaa ctttccattt gttcaagaaa
    29701 ggggtatgtt gtgaaaaatc gtttagaaga attacgaaaa caaagaggta ttaaacaaga
    29761 agatttagcg actgctcttg aagtatctcg tcaaacaata ggctctttag aaaatggacg
    29821 ctacaatccc tctattatct tagcttttaa aattgcaagg tactttcaaa tgagtattga
    29881 agaaatattt atttatgagg aggaaagcaa atgaaacata agatacaaaa agttgaatta
    29941 tttatcggtg taataatctt tttaggtggt ttactaactt atggtttagg cgtacaccaa
    30001 ctattgcccg tacctcgtcc agatttggtt gtctatggta caacgcttat tggaataata
    30061 ttgattttta tgggttgtga tatattcagt aagccaacaa aagaaatgca aatattagaa
    30121 aatgatgaaa gaaatatcgc aattactaat gcttctcttg cgaatgctta taaagtaaca
    30181 cttacattat tggttcttgt attatttgct cttgtcttta tgggatatat gagtaaagtt
    30241 gtgttttttt cgatagcagg agttattgct atcggacaaa ttacatgggt tataacagct
    30301 cgttatttag ataaaaatac gtcttaattc ttcaagtatt tttagttttt ccttctccat
    30361 aactacgccc cattcttttt ttgtatagta atattgtatc attaagaatc acaaatcaca
    30421 agtgatttgt gattaatcac ttgtgatttg tgataagtga tttgtgatta atatataaaa
    30481 gccctcttta aagggctttt atgtttattt tgaaaaagag ataaaatcaa tatatccctt
    30541 ttccccaatt tttacaacgg cattgtaggg ctttttctct ttgtttttga ttccttttac
    30601 cagggtttct tttccctcca gtaattcttt tacattcgtt ttggttagtt ttttctttct
    30661 aaaatgttca gctaaagtaa acttacattc aggataattt gaacaaccat aaaacgattt
    30721 ttttaataca atattgttgc cacacttagg acattttcct acaaggggtc ccgagcgctt
    30781 agtgggaatt tgtacccctt atcgatacaa attccccgta ggcgctaggg acctctttag
    30841 cttcttggaa gctgtcagta gtatatctaa taatttatct ccattccctt tagtaacgtg
    30901 taactttcca aatttaaaaa agcgactcat agaattattt cctcccgtta aataatagat
    30961 aactattaaa aatagacaat acttgctcat aagtaacggt acttaaattg tttactttgg
    31021 cgtgtttcat tgcttgatga aactgatttt tagtaaacag ttgacgatat tctcgattga
    31081 cccattttga aacaaagtac gtatatagct tccaatattt atctggaaca tctgtggtat
    31141 ggcgggtaag ttttattaag acactgttta cttttggttt aggatgaaag cattccgctg
    31201 gcagcttaag caattgctga atcgagactt gagtgtgcaa gagcaaccct agtgttcggt
    31261 gaatatccaa ggtacgcttg tagaatcctt cttcaacaat cagatagatg tcagacgcat
    31321 ggctttcaaa aaccactttt ttaataattt gtgtgcttaa atggtaagga atactcccaa
    31381 caattttata cctctgtttg ttagggaatt gaaactgtag aatatcttgg tgaattaaag
    31441 tgacacgaat gttcagtttt aatttttctg acgataagtt gaatagatga ctgtctaatt
    31501 caatagacgt tacctgttta cttattttag ccagtttcgt cgttaaatgc cctttacctg
    31561 ttccaatttc gtaaacggta tcggtttctt ttaaattcaa ttgttttatt atttggttga
    31621 gtactttttc actcgttaaa aagttttgag aatattttat atttttgttc atgtaatcac
    31681 tcctgaagtg attacatctg taaataaata cagaagttaa acgatttgtt tgtaatttta
    31741 gttatctgtt taaaaagtca taagattagt cactggtagg aattaatcta acgtatttat
    31801 ttatctgcgt aatcactgtt tttagtctgt ttcaaaacag tagatgtttt atctacatta
    31861 cgcatttgga ataccaacat gacgaatccc tccttcttaa ttacaaattt ttagcatcta
    31921 atttaacttc aattcctatt atacacaaaa ttttaagata ctgcaatatc aacacactct
    31981 taagtttgct tctaagtctt atttccataa cttcttttac gtttccgcca ttctttgctg
    32041 tttcgatttt tatgatatgg tgcaagtcag cacgaacacg aaccgtctta tctcccatta
    32101 tatctttttt tgcactgatt ggtgtatcat ttcgtttttc tttttgtgcg cctaaatttc
    32161 ccacaatcac tcacttcttt ctatttcttc ttattcttat tttatcatca acaatcacaa
    32221 atcacttgtg atttgtgata agtgatttgt gataagtgat ttgtgattaa tatataaaag
    32281 ccctctttaa agggctttta tgtttatttt gagaaagata taaaatcaat atatcccttt
    32341 tccccaattt ttacaacggc attgtagggc tttt
//
LOCUS       DAGQWR010000030        32256 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.030, whole genome shotgun sequence.
ACCESSION   DAGQWR010000030 DAGQWR010000000
VERSION     DAGQWR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32256
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.030"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..1733
                     /locus_tag="MIW95_001205"
     CDS             <1..1733
                     /locus_tag="MIW95_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150548.1"
                     /translation="EITKLKTSIEKIEEEKKVIKKSIEDIDVNIFNLNSEKDRINRHI
                     DDTESKINKFKIYEPIKLENAKLEELEIKYKKYLEDPTKKEIETLNYEISVCLKRIES
                     ITIELEEFRQLISSDNFENIPILFTIEELNKRIVLNKSKVDELRKLKYSIKSDIDKNQ
                     GSLNTKREEILKKFNGKEPLSTSLIVNFNFEERKIDIKKDIKDIGEIIEGNRTTCQNL
                     EQELRDLKKYKVERKAITYQPEDIFIESKKLQSKLQNVEYNLIKSINSYKNTILEIKE
                     FNYKHMEKYKNFIETLNVNKDNYSRQKEQVEIVLLVIREEISKLEKHSEQLRKEKTII
                     SRQIKDYVMDCIDEFKIINKLGKHKGQALFNISLPKSDKIENSLSLVDMLMDEIVEEA
                     NLGDVEQKINTFYILNKTLNIGRIPVSVIKYEINRNETIKWNSINNETTGGQRFCISF
                     IVTILLMEYKRYDRNAIVDESKYKGKVLLMDNPFGETSQQDFLKEIFELAKKFNVQII
                     SYTHVTNASIRAMFNKIYLMTVEKTTSNKEFVDINEIKQNKTDELVYMSKFYIGNKEE
                     LIQEDIFSLI"
     gene            1835..2044
                     /locus_tag="MIW95_001206"
     CDS             1835..2044
                     /locus_tag="MIW95_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150549.1"
                     /translation="MKNFVIVSTKAALLRVIIVRLLLKIGLKTYKYLSYFMKKEKLKR
                     IIRPEGCELRVNRSIQVERAIAEQI"
     gene            2440..2580
                     /locus_tag="MIW95_001207"
     CDS             2440..2580
                     /locus_tag="MIW95_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3150550.1"
                     /translation="MKIKIKKYREKLLMIQNELAKSVGVRRETIVHLENGLMYHKGIQ
                     DK"
     gene            2718..3680
                     /locus_tag="MIW95_001208"
     CDS             2718..3680
                     /locus_tag="MIW95_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBY3150551.1"
                     /translation="MAKIDNMMSILWMLNSDKKVTAKQISEKLEINIRTVYRHIDALS
                     ASGVPIISDTGHNGGYTLLNNFIKAPLFFDTEEQTALLHAAIFAKEEGSFLDDTLDKA
                     TSKLKMYSNQKQEETLTKHLIGFEVIKPTGKISIESVLKKLKNSIANELSVEINYRTA
                     HEEKSKCRIINPYGIVRWNNNWYIVAFCQMRNEIRSFRVDRISSVIETSNTFNRPKEF
                     SASEFFIRGIMDETKDEDNLISLVIEGKMNALDNLCQHWLLGYYLKERTSNKAIFKLQ
                     EEIIFTYVPNILIPYRKSIQIIEPLSLRDKMVEDLLELINHYQI"
     gene            3758..4387
                     /locus_tag="MIW95_001209"
     CDS             3758..4387
                     /locus_tag="MIW95_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBY3150552.1"
                     /translation="MKKRKVYMYVFDTMSDWEVGYLTAELNTGRYFKKGLKPLKVITV
                     GVDKNPIITMGGLKVLPEITIDELNIESNDLLILPGGNTWTSIGHEKILEKSKEALEQ
                     RSIVAAICGATLGLAKTGLLDFRNHTSNDLEFLKMVILSYSGEKYYKMESAVNDENLI
                     TASGIAPLEFTVQVLKVLDVFKEEALSSWLNLYKTHDSKYFFELMNCNK"
     gene            5218..5634
                     /locus_tag="MIW95_001210"
     CDS             5218..5634
                     /locus_tag="MIW95_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150553.1"
                     /translation="MSKSETGVKSKLILELLVITYLFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSKFILSKVNQIAITIMMYSIVIIAIFFATPYISNI
                     NISNLDTGIILIAVLFIQSLSKLILYIYFDKRGICN"
     gene            5635..5847
                     /locus_tag="MIW95_001211"
     CDS             5635..5847
                     /locus_tag="MIW95_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3150554.1"
                     /translation="MAISMKTKIKEYREKLLMTQNELAKSVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFGTTVENLFEFIEED"
     gene            6482..7525
                     /locus_tag="MIW95_001212"
     CDS             6482..7525
                     /locus_tag="MIW95_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBY3150555.1"
                     /translation="MKRKIAVVLLTVAMLLSLVACSNDNTSKSDKLEDTLVVYTTHGE
                     DMLGAISEEFTKETGVNVEFINLKGELADRVRSEKDNPQADIMFGGDTSTYMQLKEEG
                     LYEKTEPSWNNELDKSYKDSDGYWYGTIKTPVMMFYNTKLLSADKAPKDWSDLTKEEY
                     KDLIVTRDSLSSSMRSTISNLINYYSNTKSEEEAWKYLEDLNKNIKNYYNSGSVMYSA
                     IGKGEATISVAVLSDIIENKEKNNMPLEVVDAESGSVVITDCVAALKNAPHPNAAAKF
                     VDFVGSAKVQAMVANDFNRIPTLDSALKDSPKWMSKEYKAMDVDWSVIGKNQSNWIEK
                     WETDIIDNNKTIK"
     gene            7786..8660
                     /locus_tag="MIW95_001213"
                     /pseudo
     CDS             7786..8660
                     /locus_tag="MIW95_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8679..9671
                     /locus_tag="MIW95_001214"
     CDS             8679..9671
                     /locus_tag="MIW95_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3150556.1"
                     /translation="MKNIELKEINHYYGKEKVLHDINISIKEGEFFTLLGPSGCGKTT
                     ILRIIGGFIKPSSGSIYVGDKNITNLEPENRNMGTVFQNYALFPNMTVEENVSYGLKI
                     KKLSKKIIKEKCDNYLELSGMKDFRKKKIDELSGGQQQRVAVARSLATEPTMLLLDEP
                     MSNLDIALRIKMREEIREIQQKTGITTLFITHDQQEALAISDRIAVMDKGKVLQIGVP
                     IEVYKSPLNDFVANFVGTSNCIGKEDYVNFNIEKETKPYIYKRPEEMILLQNTNQSGF
                     VKVKVESKNFLGAILEYTVSNDGKKYQVTELNRLNNSNKLNVGDMAYLGVLQGD"
     gene            9673..11367
                     /locus_tag="MIW95_001215"
     CDS             9673..11367
                     /locus_tag="MIW95_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBY3150557.1"
                     /translation="MIKKINRSKFTSKNVIFIVLYSILFWILIGYIIIPIGNTFVQAF
                     QGKGGYSFEVFKEYFSNTNNLRVVSNTIILGLGSVFVCGIMGISLALYMTFVCDKHKK
                     IIHILLLSPMMIPGVIMVIACIQIYGESGIVTKSIEILLPFIKKPYDFSGLKGILFVV
                     AYTQYVYFYLNVYVALKYVDYSTVEAAKGMGASKLRVFFDVIWPVITPAVITSTIITF
                     ASGISSFSAPNLIGGGFKVLSTQIVRSKANNHMDIASVQVITLLLMGILFMILLQYYG
                     KKYGVVPSLKSQSYKVHNRKKDLFTFVCKVIIEIQIIMIMLPIVTIVYLSFVSTHSIM
                     TTIFPRDFTLENYQMIFQKSRVLQPILNSFKMSLMAVAVGLIITVPVSYLVIKKDTAY
                     NRLAKFIIMLPWSMPASAIAVNLINTFNQKSIFAFNESLIGGFYIVPIAYTIYALPLL
                     FSSNEVAMKSINLGLEESSRSLGAGRIKTFYNVIVPNMMPGIISGAILVFIRTIGEYT
                     MSALLYGVYNRPISISIVTNMQEFKIGVSMAYGVLVIGICYIALGIIFKLDRKRFT"
     gene            11918..12796
                     /locus_tag="MIW95_001216"
     CDS             11918..12796
                     /locus_tag="MIW95_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBY3150558.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQGANEKNAPV
                     ILAIHPNELSFLGDSFVVSCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            12832..14727
                     /locus_tag="MIW95_001217"
     CDS             12832..14727
                     /locus_tag="MIW95_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY3150559.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSETKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFPPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVAKPTIRSENS
                     EVELLGA"
     gene            14995..16902
                     /locus_tag="MIW95_001218"
     CDS             14995..16902
                     /locus_tag="MIW95_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY3150560.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDILSESEKVSSIMIENMSQFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            16892..17341
                     /locus_tag="MIW95_001219"
     CDS             16892..17341
                     /locus_tag="MIW95_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY3150561.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNISERLLNVHTKKEILDIFYEV"
     gene            17441..18328
                     /locus_tag="MIW95_001220"
     CDS             17441..18328
                     /locus_tag="MIW95_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY3150562.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGGASK"
     gene            18733..20181
                     /locus_tag="MIW95_001221"
     CDS             18733..20181
                     /locus_tag="MIW95_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBY3150563.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIIHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            20207..22597
                     /locus_tag="MIW95_001222"
     CDS             20207..22597
                     /locus_tag="MIW95_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBY3150564.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGIVKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIELKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKDWI
                     NAAQNIYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLNYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKKDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGKNISILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            22599..23270
                     /gene="pgmB"
                     /locus_tag="MIW95_001223"
     CDS             22599..23270
                     /gene="pgmB"
                     /locus_tag="MIW95_001223"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBY3150565.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLERGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            23309..24709
                     /locus_tag="MIW95_001224"
     CDS             23309..24709
                     /locus_tag="MIW95_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY3150566.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNENKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFINKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKESTDNVVEEV"
     gene            25026..25868
                     /locus_tag="MIW95_001225"
     CDS             25026..25868
                     /locus_tag="MIW95_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY3150567.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            26013..27941
                     /locus_tag="MIW95_001226"
     CDS             26013..27941
                     /locus_tag="MIW95_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY3150568.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKFVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMTIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVASPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            28002..29429
                     /gene="ascB"
                     /locus_tag="MIW95_001227"
     CDS             28002..29429
                     /gene="ascB"
                     /locus_tag="MIW95_001227"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBY3150569.1"
                     /translation="MSFKDDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYRGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLVGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     INYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            29840..30616
                     /locus_tag="MIW95_001228"
     CDS             29840..30616
                     /locus_tag="MIW95_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY3150570.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            30632..30997
                     /locus_tag="MIW95_001229"
     CDS             30632..30997
                     /locus_tag="MIW95_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBY3150571.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(31163..31912)
                     /locus_tag="MIW95_001230"
     CDS             complement(31163..31912)
                     /locus_tag="MIW95_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBY3150572.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     GKPFIFVCPTYAWRLPIVVTDFIKETKFLGDNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNS
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
BASE COUNT        12942 a         3369 c         5561 g        10384 t
ORIGIN      
        1 atgaaataac taagttaaaa acaagtattg aaaaaatcga agaagaaaaa aaagttatta
       61 aaaaatcaat agaagatatt gatgttaata tatttaattt aaattcagaa aaagatagga
      121 taaataggca tatagatgat acagaaagca agataaataa atttaagatt tatgaaccta
      181 ttaaacttga aaatgctaaa ctagaagaat tagaaataaa atacaagaaa tacttagaag
      241 accctacaaa aaaagagatt gaaacattaa attatgaaat ctcagtttgc ttaaaaagaa
      301 tagagagtat tactatagag ttagaagaat ttagacaact tattagtagt gataattttg
      361 aaaatatacc tatattattc acaatagaag aacttaataa aagaatagtt ttaaataaaa
      421 gtaaggttga tgaactacga aaacttaaat atagtattaa aagcgacata gataaaaatc
      481 aaggttcatt aaacactaag agggaggaga tacttaaaaa atttaatgga aaagaacctt
      541 tatctactag cttaatagtg aactttaatt ttgaagaaag aaaaatagat ataaaaaaag
      601 atataaagga tattggtgaa attatagaag gtaatagaac tacttgtcaa aatctagaac
      661 aggaattaag agacttaaag aaatataaag tagaaagaaa agctataacc tatcaacctg
      721 aagatatttt tattgaaagt aaaaaactac aatcaaaact gcaaaatgtt gaatataatt
      781 taattaaatc tataaatagc tataaaaata caatattaga aataaaagaa tttaattata
      841 agcacatgga aaaatacaaa aactttatag aaactttaaa tgtaaataaa gataattact
      901 ctcgacaaaa agaacaagta gaaatagtat tactagttat aagagaagaa ataagcaaat
      961 tagaaaaaca tagcgaacaa ctaagaaaag aaaagactat aatatcaagg caaataaaag
     1021 attatgttat ggactgtatt gatgaattta aaatcataaa taaacttggt aaacataaag
     1081 gacaagcctt attcaacata tcactaccta aatctgataa aatagaaaat agtttgagct
     1141 tagtagatat gctaatggat gaaattgtag aagaagcaaa cctaggtgat gttgaacaaa
     1201 aaataaatac cttttatatt ttaaataaga ctcttaatat agggagaatt cctgtatcag
     1261 ttataaaata tgaaataaat agaaatgaaa ctataaagtg gaactctatt aataacgaaa
     1321 caacaggagg acagagattt tgtatctcat ttattgtgac aatattgttg atggaatata
     1381 aaagatatga tagaaatgca atagttgatg aaagtaaata taagggtaag gtactactta
     1441 tggataatcc ttttggagaa acgtcgcagc aagatttcct taaagaaata tttgaacttg
     1501 ctaaaaaatt caacgttcaa attatatcct atactcatgt aacaaatgct tctataagag
     1561 caatgtttaa taagatttat ttaatgacag ttgaaaaaac tacttctaat aaagaatttg
     1621 tggatattaa tgagattaag caaaataaaa ctgatgaatt ggtgtatatg agtaaatttt
     1681 atataggtaa taaagaagaa ttaattcaag aggatatatt tagtttaata taaataacaa
     1741 gtaaacaaat aaagtgatta ctatcagtga ggcaggtaga aactgaatta ctttgtgact
     1801 cattacgact cttggggtaa aattgtgtat aaatgtgaag aatttcgtga ttgtcagtac
     1861 aaaagcagct ttattaaggg taataatagt aagactcctc ttgaaaatag gactaaaaac
     1921 ttacaagtat ctaagttatt tcatgaaaaa agaaaaatta aagagaatta taagaccaga
     1981 gggatgtgaa cttagagtga atagaagtat acaagtagaa agagctatcg cagaacaaat
     2041 ttaattttat catacgatag cctcttgtta ttttagtgat gaaatccatc aagcatacca
     2101 taactacaga aaaagaggct tttctgatgg tatattgggt tcttttagag tgtgtgattt
     2161 gctacatgtt cattaggata ataatagtca gtaaatgata ttactgatag aatagatata
     2221 ataaactaat ctagttccat attgtaatgt ttattaaaga gtatactttt atgattataa
     2281 tagctatttt ctttgaagct tattgtacaa gagatttgaa tatatcaaat ttggatatag
     2341 gtataattct cttagcagtt ttatttatac agtctttatc taaattagtg ttgtatatac
     2401 tttgataaaa gggggatatg taactaagtt gtaatattga tgaaaataaa aattaaaaaa
     2461 tatagagaaa aactccttat gatacaaaat gaacttgcta aatcagttgg ggtaagacga
     2521 gagactattg tacacttaga aaatggatta atgtaccata aaggtatcca agataaatag
     2581 ttaaaaaact agataagaat aaggagcctt tacagaacaa atttagtgtg ctcgtgcgat
     2641 agcccttttt ttataaaaat taatctaata acctatataa ttaaatttat aaaacatata
     2701 tgaaagggta aaactatatg gctaaaattg ataatatgat gtccatatta tggatgttaa
     2761 actcagacaa aaaagttacc gcaaaacaaa tatctgaaaa actagaaata aatataagaa
     2821 ctgtctacag acacattgat gcattatctg ctagtggagt acctataatt tcagatacag
     2881 gtcataatgg tggatataca cttttgaata atttcatcaa agcacctctt tttttcgata
     2941 cagaagaaca aactgcgtta cttcatgcag ctatatttgc taaagaagag ggaagtttct
     3001 tagatgacac actagacaaa gcaacctcaa agttaaaaat gtattcaaat caaaaacaag
     3061 aagaaacttt aacaaaacat ttaataggtt ttgaagtaat aaaaccaact ggaaaaatat
     3121 ccatagaatc tgtacttaaa aagttaaaaa atagtatagc aaatgaatta tctgtagaaa
     3181 tcaactatcg tacagctcat gaagaaaaat caaaatgtag gattataaat ccatatggaa
     3241 ttgtacgttg gaataataat tggtacatag tagcattttg tcaaatgaga aatgagattc
     3301 gtagttttag ggtagataga attagtagtg tcatagaaac atcaaatact tttaataggc
     3361 caaaagaatt ttcagcaagt gaatttttta taagagggat tatggatgaa acaaaagatg
     3421 aggataatct tatatcatta gttatagaag gaaagatgaa tgcattggat aacttatgtc
     3481 agcattggct tctaggatat tatttaaaag aaaggacttc aaataaagca atatttaaac
     3541 ttcaagaaga gataatattt acatatgtac cgaatatact tataccttat agaaaatcta
     3601 tacaaataat tgaaccatta agccttaggg ataaaatggt tgaagattta ctcgaattaa
     3661 ttaatcatta tcaaatttaa taacttaact gacagagtat gtcagttaag ttattttaca
     3721 attaggtata ttaattaatt atagaaggag caaaaaaatg aaaaagagaa aagtatatat
     3781 gtatgtattc gatactatgt cagattggga agttggatat ctaactgctg aattaaatac
     3841 aggaagatat tttaaaaaag gattaaaacc actaaaagta attacagtag gagttgataa
     3901 aaatcctatt ataacaatgg gtggattaaa agttttacct gagattacaa ttgacgagct
     3961 taatattgaa agtaatgact tattaatttt gccaggggga aatacttgga catctatagg
     4021 tcatgagaaa atattagaaa aatctaaaga ggctttggaa caaaggtcta tagttgcagc
     4081 tatttgtggt gctactttag ggttagctaa aacaggttta ctagatttta gaaatcatac
     4141 aagtaatgat ttagaatttt taaaaatggt gatactaagt tattctggag aaaaatatta
     4201 taaaatggaa tctgcggtta atgatgaaaa tttaattact gcttctggaa ttgcaccatt
     4261 agaatttaca gtacaagttt taaaagtttt agatgttttt aaagaagaag cattaagttc
     4321 ttggttgaac ctttataaga cgcatgactc taaatatttt ttcgaactaa tgaattgtaa
     4381 taaataaatt taagatttaa ttttaaattc aaattttaaa ttactttttc tatggcaata
     4441 gataagtagt ttagaatttt atcatatata taatttgtat ttataccaac acaaatagaa
     4501 atgatagttc caataataaa atttataatt agttccatat ggagtacatt tctttcctac
     4561 taatataact aagaatatat gaaaccacca tagattgtag ttttttctgt tgtattatat
     4621 catgggatag taaagtgacg tatatttttg atgaaaacct aggattgtat atgaaatgaa
     4681 ttaatcctgt gtattaatct tgagattcgt gtctaatgat ttgtaaaaat aaataatgaa
     4741 atacttagaa atacttgtaa atgctagtca atattggcta gtatttttta ttttagtaat
     4801 gaaatcccca actgtacttc atccacaaaa aaagagatat ttttgagggt gtattggagt
     4861 gaacttaggc tgtataatta tacatttcaa tatcttatat taattaggat aatagttggt
     4921 aaatgatatg attgaaacaa tagatataat aaactaatct agtactgtac tgcttactaa
     4981 agaatatatt tttatggtat aagaattttt tagagcgttg attctatagg agtgaataaa
     5041 aaagtaattt tataatatta tttgtaacca ttagatgtct attatgaaat cttttatgga
     5101 ctatatttgt aaaaatatta ttaagtattt acaatatttt cttgcaattt atttggaaag
     5161 gttatatatt tataacatga agtgttataa atatataacc tttaaggtgg attttatatg
     5221 tctaaatcag aaactggtgt aaaatcaaaa ctcatattgg aattacttgt aataacttat
     5281 ctttttatag ctgtattagc tactaagtat atttttaaag gtccaagtac tgcatctctt
     5341 ccaggactga gctggttaat agtctttgga tgctattcaa tttataataa aaaatataat
     5401 gatttagttg atgaatcatc taaatttata ctatcaaagg ttaatcagat tgcaataaca
     5461 ataatgatgt attctatagt tataatagct attttctttg caacacctta tataagtaat
     5521 ataaatatat caaacttgga tactggtata attcttattg cagttttatt tatacaatcg
     5581 ttatctaaat taattttgta tatatacttt gataaaaggg ggatatgtaa ctaagtggca
     5641 atatcgatga aaacaaaaat taaagaatat agagaaaaac tccttatgac acaaaacgaa
     5701 cttgctaaat cagttggggt aagacgtgag actattgtac acttagaaaa tggaaaatat
     5761 aatccttctt taaagttagc gatggatatt gctaaagtat tcgggaccac agtagaaaat
     5821 ttgtttgaat ttattgaaga agattagtag ataggatata aatatttata agattgttaa
     5881 taaaatatag ttatttaatt attaattttg ttaaaaatat ttataaataa tataatgtat
     5941 gagtaaagtt aggttgtaat tattttaaga taagaagact actgtaaatg ataaaaatta
     6001 gtgaatatac agccaattaa taaataatag ataaaacttt aaagcttaga tgtgaggatg
     6061 agatatggtc ttacagtagt tcacactttt aattaagact taaaatggaa accatacaca
     6121 tacatacact atttactgaa agtggactag ataaaaaaat aataagtgat ttaagaaagt
     6181 atctcatata ctatataagt attattttag gtgacctatt tataggtcat tttttatgta
     6241 tataaatgat aataatacta atatttaaaa taggttttaa agaaataaaa tatccagatt
     6301 gaaaaatgga ttaatgttaa taaaaaaatt atatgtataa aaataggtat aattatagaa
     6361 aaaaacaata ataaaataag ctgttataga aaaatacaat tatatagatt gttgttgaat
     6421 cattgactgc aagaataata acatttaaaa tgaacatgga gaatatgggg gaggaatcaa
     6481 tatgaagaga aaaatagcag ttgttttatt aactgtagca atgttattat cacttgtagc
     6541 ttgtagcaat gataacacat caaaaagtga taaattagag gatacattag tagtatatac
     6601 tacacatgga gaggatatgc taggagcaat ttctgaagaa tttacaaagg aaacaggagt
     6661 taatgttgaa tttataaact taaaaggtga actagcagac cgtgttagaa gtgaaaaaga
     6721 taatccgcaa gcagatataa tgtttggtgg agatacatct acatacatgc aattaaaaga
     6781 agaaggatta tatgaaaaga ctgaaccaag ctggaataat gaattagaca agtcttacaa
     6841 agattcagat ggatattggt atggaactat aaaaacacca gtaatgatgt tttataacac
     6901 aaaactatta tcagctgata aagcgccaaa agattggagt gatttaacaa aagaagaata
     6961 caaagactta atagttacta gagattcatt atcatcatct atgcgttcta ctattagtaa
     7021 cttaatcaat tactactcaa atactaagag tgaagaagaa gcttggaagt atcttgagga
     7081 tttaaataag aatataaaga attactacaa tagtggaagt gtcatgtatt cagcaatagg
     7141 taaaggtgaa gctacaatat ctgttgcagt attaagtgat ataatagaga ataaagaaaa
     7201 aaataatatg ccattagaag ttgtagatgc agagagcggt tctgtagtaa tcacagactg
     7261 tgtagcagct ctaaaaaatg caccacatcc aaatgcagca gctaaatttg tagattttgt
     7321 tggaagtgct aaggttcaag ctatggttgc aaatgatttt aatcgtattc caacattaga
     7381 ttctgcactt aaagattcac caaagtggat gtcaaaagaa tataaagcta tggatgtaga
     7441 ttggtctgta attggaaaaa atcagtctaa ctggattgaa aagtgggaaa cagatataat
     7501 agataacaat aaaactataa aatagcctaa ttaaataatt taatattata tacaaaaata
     7561 gtgaggattt tcctcgctat tttacgtata aggaaactat agtatctcat aattttgtat
     7621 aattcataca acaaaatgat actaatacat acttattaaa taaaatagtt tttgagtaac
     7681 tgatgaagta attgactata taaagtgatt tgtagacata gtctctcaaa taaagtgaat
     7741 tttttattat gcaaaacttt taattatagt aaaagtacaa tttatatggg caaggagata
     7801 aaaatggaac taaaaattag tgattataaa ttattatgta ataaaggtag tggcgctttt
     7861 aacgaagata tcgtaggttt atcaccttat ggggcatggg tacttgatgg agctacaggt
     7921 ttaaataata aaaatttagt ctctacagaa agtgatgcca aatggtatgt aagttggtgg
     7981 aatagttatc ttcatgagaa tatggaaaga gaagatagct taaaagtaat tattaaagaa
     8041 ggcattgagg agataacaaa agagtactac aaattaacag atgataaaga agtagaagca
     8101 atagatattc cttcttgttc atgttctata ttaaagtttt ataaagataa gatagagtat
     8161 ttacttttag gagattgtac tttattagtt aacaataatg aatctgtgaa aattttaaaa
     8221 gatgagactt tatgtgaact tgataaaaaa gtatttgatt ttatgaaaga agccaaagat
     8281 gctacaaacc ttactttaaa ggagagaaaa gccgaggtaa tgcgattata atagagaata
     8341 gacttaagaa aaatactgtg gggggatatt ggactttgga attttctaaa gaggccgttg
     8401 acaattgtgt aagtggattt attgatatag aatataaaat gaaaattatg atggctagtg
     8461 atggtttttc atgtggatat gatagatata agatttttga taaaaatgaa atgatgaaaa
     8521 tagcagagat taaaggtata gattatatat atagaaaggt tagggaacta gaaaaggatg
     8581 atgaaatggc aatagagttt cctagattta aaatctatga tgattcatca tgtgtatatt
     8641 tagagattta taaatattag ataatttatg gagacgatat gaagaatata gaattaaaag
     8701 aaattaatca ttattatgga aaagaaaaag tattacatga tattaatata tctattaagg
     8761 aaggagagtt ttttacttta ctaggtcctt ctggatgtgg aaaaacgact attttaagaa
     8821 taattggtgg atttataaaa ccttcttctg gaagtattta tgttggtgat aaaaacatta
     8881 ccaatttaga accagaaaat agaaatatgg gaactgtttt tcaaaactat gcactgtttc
     8941 caaatatgac agttgaagaa aatgtatctt atggattgaa aataaaaaag ctgtctaaaa
     9001 aaattataaa ggaaaaatgt gataattatt tagagctgtc aggaatgaaa gattttagga
     9061 aaaaaaagat tgatgaatta tctggtggac agcaacaaag ggtggcagta gcaaggtcat
     9121 tagcgactga gcctactatg cttttattag atgagccaat gtctaatcta gatatagctt
     9181 taagaatcaa gatgagagaa gaaataagag agatacagca aaagacaggt attactactt
     9241 tatttattac ccatgatcaa caagaagcct tagctatttc tgatagaatc gctgtaatgg
     9301 ataagggtaa agttcttcaa attggtgtgc caatagaagt ttacaaaagc ccattgaatg
     9361 attttgtagc aaactttgta ggtacttcaa actgtattgg aaaagaagat tatgttaatt
     9421 ttaatattga aaaagagaca aagccttata tttataaaag acctgaagaa atgatattat
     9481 tgcaaaatac aaatcaaagt ggttttgtaa aagtaaaagt agaatctaag aattttctag
     9541 gagcaatttt agaatataca gtatcaaatg atgggaaaaa atatcaagta actgagctta
     9601 atagactgaa taatagcaat aaattaaacg taggggatat ggcatattta ggtgtgctac
     9661 aaggtgacta atatgattaa aaaaataaac cgtagtaaat ttacatctaa aaatgttatt
     9721 tttattgttt tatacagtat attattttgg attttgattg gatatattat cataccaata
     9781 ggcaatactt ttgtacaagc attccaaggt aaaggtggat attcttttga agtttttaaa
     9841 gaatattttt caaatactaa taatttaagg gtagtaagca atactataat tttgggattg
     9901 ggttctgtgt tcgtatgtgg aattatggga atatcacttg ccttatatat gacatttgtt
     9961 tgtgataaac ataagaaaat catacatatt cttttactaa gtccaatgat gattcctgga
    10021 gtaattatgg taattgcatg tattcagatt tatggagaaa gtggaatagt tacaaaatct
    10081 atagaaattt tactcccctt tatcaaaaaa ccttatgatt ttagtggatt aaagggaatt
    10141 ttatttgtgg ttgcttatac tcagtatgta tatttttatt taaatgtata tgttgcctta
    10201 aaatatgtgg attactcaac agttgaagct gcaaaaggaa tgggagcatc aaagctaaga
    10261 gtattttttg atgtaatatg gcctgttatt acacctgctg ttataacttc tactattata
    10321 acttttgcat cagggattag ttctttctca gctccaaatc ttataggtgg tggttttaaa
    10381 gttttaagca ctcaaattgt taggtctaag gctaataacc atatggatat agcttcagtt
    10441 caagtaatta ctttattact aatggggatt ttatttatga ttttattaca gtattatggc
    10501 aaaaaatatg gagtagtgcc atccttaaaa tctcaaagtt ataaagttca taatagaaaa
    10561 aaagacttat ttacatttgt atgtaaggtt attattgaaa tacaaattat tatgattatg
    10621 cttcctatag ttactattgt gtatttatcc tttgtaagca cacattcaat tatgacaacc
    10681 atatttccta gagactttac actggaaaac tatcagatga ttttccaaaa atcacgtgta
    10741 ttacagccta tattaaatag tttcaaaatg tctttaatgg cagtagcagt aggattgata
    10801 attactgtac cagtatcata tttagtaatt aaaaaggata ctgcatataa tcgactagca
    10861 aaatttatta taatgttacc ttggagtatg cctgcaagcg ccattgcagt taacctaatt
    10921 aatacattta atcaaaaaag tatatttgct tttaatgaat ccttaatagg aggtttttat
    10981 attgtaccta tagcatatac aatttatgct ctaccattat tattttcatc caatgaggtt
    11041 gctatgaagt caataaattt aggactagaa gaatcatcaa ggagtttagg tgctggaagg
    11101 ataaaaacct tttataatgt aatagttcca aatatgatgc caggaataat ttcaggagct
    11161 atattagtat tcattcgtac aattggggaa tatacaatgt cagccttgct ttatggtgtt
    11221 tataatagac ctatttcaat ttctattgta actaatatgc aagaatttaa gataggagta
    11281 tctatggctt atggtgtatt agtaattggt atatgctata tagcattagg aataattttt
    11341 aaattagata gaaaaagatt tacataagat aacaaaggct aatttataaa ttggtgctta
    11401 gaaaaccaaa tcactttatt agctaaagat gaatttgaag ggtgacactt tggtgtttta
    11461 tgtaaagttt atggaagtaa aacattaaat aatcgtgagt atgattccta taaatcgtat
    11521 atactattga aatccaagaa aaggtattgg ctaaatagct aatatctttt taattttttg
    11581 actcccatat agcattaact attacatgag tatctttaaa tttatagtta aagtatctta
    11641 attgtttatt ataaaaatat aggatggcta aagcacatta aattaaaaca cttaaaaaga
    11701 gaagatgtac atgagaaata taattaacta tgatacagac ttctaaaaat tatttagtga
    11761 aaaggaactt agagtgtata ataaaagttc cttttcaata aggaaaacga ttgttatttt
    11821 ctgagcttca ttaccctgat attatttaaa tataaacaac aacaaaatta acaagaagta
    11881 aattcaacct aaaatttaaa atatcgggag gtacaatatg ttaatcaata tgaaggaaat
    11941 gctaaaagtg gcacaagaaa atcaatttgc tgtaccagca tttaatatag gaagtgggca
    12001 aatacttaaa gcagtagtac agggtgctaa cgaaaaaaat gcaccagtaa tacttgcaat
    12061 acacccaaat gagctaagtt ttctaggcga tagctttgta gttagttgca tagaagaagc
    12121 aaataagagt aaagtaccta tggtaataca cttagatcat ggggaaaata aagaacaaat
    12181 attaagggca ataagatgtg gatttacatc agtaatgatt gatggctcac atttaccata
    12241 tgaagagaat gtagctatat ctagggaaat agtagaaata gcaaaaggat taaatgtgtc
    12301 cgtagaagga gaacttggaa caataggaac aacaggaaca tcaagtgaag gtggtactga
    12361 tgaaataata tatacagatt caaaattagc taaggatttt gttgaaaaaa caggagtaga
    12421 cacactagct attgcaattg gtacagcaca tggtatatat ccaaaaggtt ttaaaccaga
    12481 gcttaaacta gatttattaa aagaaataag agaagtagta gatatacctt tagttcttca
    12541 tggtggttca tcaaatccag atgaagaaat agcacaagca gttaaactag gagtttgtaa
    12601 aataaatatt tcgagtgatg ttaagtcggc atattataag aagtgcagag aactattaga
    12661 acaaaaccca agcttatatg agccagatac tatatatcca ccatgtataa aagctgcaag
    12721 agaagttata gagtttaaaa tgaatctatt caatgctata gataagttaa agtgctttta
    12781 taaaaataaa aattaattat gaggtaacaa attaaaagga ggggagaatt tatgataggt
    12841 aaaatactac gaagcgattg tattgtacta gataaacaat tacaaacaaa aaaagatgta
    12901 attaattata tggcaaatca tttgtaccat ggaaattata taaataataa agaaaagttt
    12961 atagctgata ttaataaacg tgaaaaagaa tcatcaacta atatgggtaa tttaatagct
    13021 atacctcatg ctaagtcaaa tgtagtaaat caacctgtaa tgatgtattt aagaacagat
    13081 agagagatta cagataatga tggtcaaaaa cttaggtcat tttttatgat agcagttcca
    13141 gaatcaggtt cagatgaaca tcttaagatt atatctaaac tagctacata tctaatggaa
    13201 gaaaattttc ttaagcatct actaagtgca gaaactccat acgaaatatt aagtatattt
    13261 aataataaag ctgcagaatc aggagatttt agtaataatc aaaataaagg tcttatagtt
    13321 gggataacag gatgtccaac aggtatagct catactttta tggcaaaaaa agcattagag
    13381 gaagcagggc aagaacttgg atatgaagtt aaaatagaaa ctcatggagc tataggtgta
    13441 gaaaataagt tgactaaaga agatataaaa agagctgaat acgtaataat agcttgtgat
    13501 aaagaagttc aactgagtag atttatagga aagaaagtgc tacaaaagaa agtagtagat
    13561 gctaagaatg tttcattagc taaaaattta attataaacg ctgaaaatgg aatggctaaa
    13621 gaaattaaac caggtggaaa gactttagaa gattcaaaag aaatgcttca agggtcagag
    13681 acaaagggtg tttataagca cttaatgagt ggtgtatctg aaatgattcc atttgtagtg
    13741 ataggtggaa ttggtatagc catagcattt atgtttggta tatttgcatc agacccaact
    13801 catgaaacat acaaccctat agctggattc tttggacaac ttggaggcga ttcagcattt
    13861 aagttattta taccgatact tgctggtttt atagctaaat caatagcagg aagacaagga
    13921 tttcctcctg ctatgatagc tggttttatg gcagttattg gaggttcagg gtttataggt
    13981 ggtatggtag ctggttttgc aacaggatat atagctttag gagtaatgaa gataacagaa
    14041 aatttaccag atgtattaaa aggagtaaat gctgtattga tatgtccact tttgattgca
    14101 tttacgagtg gagctataat gttctttgta gttaatacgc cagcatcatg gttaaatgaa
    14161 gctttacaat cttggttaca aagtatgtct ggaactaata aagtattatt aggtgcaata
    14221 ttagcaggga tgatggcatc agatatggga ggacctatta ataaaacagc ttcagctttt
    14281 ggattggcta tgttttcaag taatatattt gagccatcag cagcattaat ggtaggagga
    14341 atggtacctc cattagcgat atcattagct atgttaatat tcaaaaataa gtttactaaa
    14401 gaagaaagaa agtcatctct accaactgct ataatggggg catctttcat aacagaaggc
    14461 gcaataccgt ttgcagctgc agacccatta agaatgattc cagcaaatat aattggttct
    14521 tgtataggtg gggcaatctg tatgagttta gatataactt taatggcacc acatggggga
    14581 atatttgtaa taccatttgc atgtagtgca ccagttgcat atatagcttg tatattgatt
    14641 ggtacagtaa taacagcatt gattataggg gtagcaaagc ctactataag aagtgaaaac
    14701 agtgaagttg aattacttgg tgcttaaatt ataaataata gtaaaaataa gtaatggaat
    14761 aacaacaagt atatagaata ataataagga tatttagaat cagttaacta cttctaaata
    14821 tccttaattt ttattttgaa attatattat ctattatatc aatttagtaa ggtgtatcat
    14881 aaattctttt atgatacaga agtatcttag taattttaca tagttagtaa atatgttaaa
    14941 atagtactta atataaacat agaaatatat aaacataggt agggagggct aagtatgctt
    15001 gtgtcgaggc aaattgaatt tcttaaattg atactaaacg aaaataaata tagacctata
    15061 aaatacttta aagataaatt aaatgtatct gataaaactt tacaaaaaga tttgaagatg
    15121 attgagaaat atttagaaac ttttaatata aaaattgata taaaaagagg ctatggaata
    15181 ttaatagaag atttagcaaa gaagaacata gacttaatta atagtttaaa tatacaatcg
    15241 aaggaaaata aatctttatc agtagagcag agaagaatgg agattttaaa gtatttatta
    15301 ctaaattcaa ataatataac ttcaataaat cagttagcag ataaatatta tgtaagtaaa
    15361 acttctatag taaatgattt taaatatata gaggcatgga taaaagaata caatttaaaa
    15421 cttaataaaa ctttagaagg aactaagatt ataggtaaag aagtggatat aaggaaatcg
    15481 attgccaaaa tgatggatga tttattagat gaaaattatg aacaacatga tatatctgag
    15541 ttaacaagat tagattctgc tacatttagt gcattgataa atttatttga tattgattct
    15601 attatatttg tagaaactat aattacagaa ttagaataca atttaggata tactataaat
    15661 cagccctact atataaattt aattacccat atacttatat gtttaaagag aatcgaagaa
    15721 ggtaatcaaa tagaatctca ggaagaaaga gaaatttctg tagataattt ggacgagtta
    15781 gtatataaaa atgtcactac acttatagat aaaatagaaa atagatacag agtaaaaata
    15841 acgaaagatg aaattatgta catatatatt tttttggtat catctggatt tagtagcgaa
    15901 aataatagta gttataatga tgaatcagat atattaagtg aaagtgaaaa agtttcatct
    15961 ataatgatag agaatatgtc acaattttta aatataaatc tcaaaaatga tgacttacta
    16021 caaaaaagtc tagcatctca tgtaaggcct atgttaaaca gattaagata tgatatccaa
    16081 ataaaaaacc cgttgttagg agagatacaa gagcgatttt cagatgtgct gggattatgt
    16141 atgatgacta taaatattat gacagagtat gataatctga agaatattag tatagatgaa
    16201 gtttcgtatc ttgccacata ttttcaagcg gcaatagaaa gaaatatgag tagtaaaaga
    16261 gtaatagtag tttgtcatac aggatatgga acatctcagc tccttgcagc cagattaaaa
    16321 agagaatttc ctgaatggac tatagttgat atagttccta tgcatcagtt gagtaagcga
    16381 aatttagatg atgtagactt tgtaatatct actgtaaact tagatttgaa agataaatta
    16441 catatagttg tatcagtact attaatggaa agtgatatta ataatataaa aaatgcttta
    16501 ctttctaaac ctaaaaaaag tatgaactta gatacaaact tattaggaat gtatttagaa
    16561 gataatatat attttaattt tgatgtagct cagatggaaa gtttaatagg tgtgaattta
    16621 gggagtaaat atgaatctat ttcattagga aaagatttga atatatatat acataagcaa
    16681 agtaaattaa ataaagggat aatgaatatc aataatgtta ctagaacgat tgatatttat
    16741 ttagaaatta gtaatcaaag ttttttaaag aatattttaa tggaaatatc aaatttatat
    16801 aggcaaaaga actatataaa ctatataata gattgcaaga gcaaagagga tattaaaata
    16861 ttatttaaga ataatctatt ggagaaaaca aatggaaatt agagaaatat taacagaaga
    16921 aagcattatt ttagatttga aatcaactac aaaagaaggc ataatacagg aattagtaga
    16981 taaattatac gatacaggaa gtataacttt aaaaagtaag tttataaagg cattaaaaca
    17041 tagagaaaaa ataggctcta ctagtatagg ttatcaaatg gccatgccac atggaaaaac
    17101 aaaatatgta aagaaggcat ccatagtaat aggtaaatct aaaaaaggtg tagattggcc
    17161 aagtatagat ggcttacctg taaaggtaat aattatgttt gctatgccac aatctggagg
    17221 caatgaagat atgatattaa tatctaaaat agcttacatt ttaggtggta aaaatataag
    17281 tgaaagactt ttaaatgtac atacaaaaaa agaaatatta gatatatttt atgaagtgta
    17341 ggtattgtat aaattataaa tttgaggttt ttaaaataaa atgtgtattc ttttaaatag
    17401 tatagattaa tttttagaca ctagagagga gaggatatct atggcaatat atttagaaat
    17461 gcctaaatta atacatgaat ttccttttcg tagtctatca aatgaaggag aagtattaac
    17521 aacaccacac tggcataagg aaatagaaat attacttata acagaaggtg tagtaaatct
    17581 atttataaat gacaagccta tgcaattaaa aaaaggcgag atagtgataa taaaaggagg
    17641 agatattcat tatatactgc catcaccggg aagtgaaaga ttagtatttc aatttgatat
    17701 atcttttttt aatgatttaa ttttattaaa tgaggaaaaa gaaagcttaa ttaatttatt
    17761 cagtagtata aaaaaatgta gtagagattg gtcagcaaaa ataagaaagt cagttttcga
    17821 tatattgata gatatctaca atgaagacct atacaaaata gaaggatata gttatgcaat
    17881 aaaaggtaaa atgtataatt taattcctat attatataga cagattccta gagaaattga
    17941 agaaaataca gtagaatatg aaataaactc taaagagata ttagagagat tgaacaatat
    18001 attcaaatat gtagaaaaaa attataaaca acctataaaa ttagaagatg tgtcttatga
    18061 agtaggattt agtgtatatt attttactaa atttttcaaa aaaaatacag gaaaaacatt
    18121 tataactttt ttaaatgaat atagattaga aaaagcaaag tggatattac ttaataacaa
    18181 tatatccatt gtagatttgg ttgaagaagt tggttttgga agtacaaaaa ctttttacag
    18241 agtatttaaa gaaaaaattg gagtctctcc attagaattt aaaaaaagat ataatagctt
    18301 ggagaaaaaa gggggtgcct caaaatgaac ttagttcatg aggcactttc ttttatatga
    18361 aatcaaatat attttcagta tttcaacaat gaaaattcca ttcatttata aagtctaaat
    18421 tttagttctc aatacaatct ctatttcgaa ataaatcctt ttatatatat aaatatacag
    18481 caatatatga gaataagata acaataatga ggatataaaa atgtatttgc tatatatata
    18541 attatataca tagatatcta atattgatat ttaatatgaa agtattgaaa atagacaata
    18601 ttaattatag tgagtaaaaa atataattgg atttttaaat tgaattattt aaaaggtata
    18661 gtatgaaaaa aatcttataa ctaaatatga gatagaaatt ataaaacttt atactgtgaa
    18721 agaggtagat gcatgaaatt agggaaagat tttttatggg gaggggcaat ttcagccaat
    18781 caaagtgaag gattttattt agatggagga aaaggtttat ctcagtttga taaactccca
    18841 ttaaatgata agagattaaa agatgtatgt ttagatgagg ataatatttt aaatgaaaat
    18901 aatcaatact atccaagtca tatagggatt aaattttgtg aaaattacaa agaagatata
    18961 gcaatgttag cagaaatagg ttttaaatgt tttagatttt ctatatcttg gtcaagaata
    19021 ttcccaacag gagaagaaag tgaaccaaat gaaaagggat tacaacttta tgataatata
    19081 ataaaagaat taaaaaaata taatatagag ccattaataa cgataagcca ctttgatata
    19141 ccttattctc tagttgaaaa atattctggg tgggctgata ggagagtggt taatttatat
    19201 gtaaaatatg ctgaaatttt gatgaaaaga tataaagatg atgtcaaata ctggattcct
    19261 tttaacgaaa tgaatatgat aattcatatt ccatatatag gtgctgggtt aacttttata
    19321 gctgatgaaa atcaacttca gaaaaaatat caagcagcac accatcaatt attagctaat
    19381 gcaaaaacta tagaaatagg aaggtatata aataaagaat ttaagtttgg gtgtatgtta
    19441 gctgctggaa aaacatatcc atacacatgt aaaccagaag atgtatttgc aactttagag
    19501 catgaaaggc ataatttgtt tttttctgat gttcaagtta ggggtgtgta tccaagcttt
    19561 atgaattatt atttaaaatc aaatcaaata aaattaataa taaaagagga agatttatca
    19621 acacttaagg ataatactgt agattttgta tcttttagct attattctag tgcttgtagt
    19681 tcagctagag ctgaaggttt agaaaaatca aaggcaaacg gaccagaaac cataaaaaat
    19741 ccatatcttc caaaatcaaa tagtgtatgg caaaacgacc ctgtagggtt aagaataact
    19801 atgaatcagt tatatgaaag gtatcagtta cctttattta tagtagaaaa tggactgggt
    19861 actcaagata ttgtaaatga agatggaaaa ataattgatg attatcgtat agattacatg
    19921 aaagaacata ttaaaaatat gattcttgga gtaaatgaag atggaataga gcttatagga
    19981 tatcttatgt ggggatgtat agatgtaacc agtgtaagtg aaggaaagat gacaaaacga
    20041 tatggcataa ttcatgttga ttctgatgac tatggaagag gctcatttaa aagaactaaa
    20101 aaacaaagtg ctttttggta taaaaaagta atagaaacag atggagaaat tttatttaat
    20161 gatatggaaa ctcatgcata attcatttta agaaaggagt gttattatga gtttaatatt
    20221 tactactaag gaccaactta tagagcaagg gtttagtaag gatacaataa aatccaatga
    20281 aacgttgttt acactagcta atggacattt aggagttaga gggaatattg aagagtccga
    20341 ttttaataat gagtatgtag ataatatggg tacatatgta aatggatttt atgaagaatc
    20401 tccaattaca tatggagaaa gcgcttatgg ctatgcaaaa caaaatcaga ctatctgtaa
    20461 gttacctaat gctcatatag taaatttctc tattgaagga gattgctttg atttatcaaa
    20521 aggtattaca agtgaacata ctaggatact agatttaaat gaaggaattg ttaaacgttc
    20581 atttatatgg gaaaactctc gtggtagaaa agttaaagta tctatagaaa gattagtatc
    20641 acaagatata gaagaattaa tgatgatttc atatacaata acaccattga attttaatgg
    20701 aaaaataaaa tttaaaaata aaatggaaaa tagtatggag aagtttaatt gtgaaaaagt
    20761 agatgaccca agagtggcat ctataaataa gaagcaattt aaaattaatg taaaaaaata
    20821 taacaataag tattttatga atttagttac agaaagaagt agattagggt tatggtgtgg
    20881 gtttgattca tatattactt gtggaaatat tacaaatgag acctataatg tttcagatga
    20941 atgtattgag cgagaaatag aagtctatgc caaaaaagat gaaaggttaa atttagaaat
    21001 tgttgtaggc tatagtagag tatattctga tttaaatcta aatgaagaac gtttaaaatc
    21061 attagaatgt ctggagtcgt taaaatgttt agaatcagaa gcacatttaa aatcattaga
    21121 attggtttta agagaagcat gtaatattgg atttgataaa gtcaaaaata ttcagagaaa
    21181 gaaaatgaat gagttttgga caacctctga tgtagagatt gaaggcgata aaactcttca
    21241 actgggtata aaaaccaatt tatttcatat ttatcaatca gcaggaagag atggaaagac
    21301 taatatatca gcaaaaggtc ttacaggtga tggctatgag gggcattatt tctgggatac
    21361 agaaatgtat atattaccat tttttatata tactcaacca tccattgcta gagctttatt
    21421 agattataga tactcaatct tagatgcatc aagagaaaga gctagggaac ttggaataaa
    21481 taaaggttgc ttatattcat ggagaactat aaatggtaat gaatgcagtg catattatcc
    21541 agcaggaaca gcacagtttc atataaatgc agatatagct tatggtatta gaacgtattt
    21601 tgaagcaact aatgatgaag aatttatgat gcaaaatggc ttagaaattc ttatagaaac
    21661 agcaagattt tgggttgaat ttggagatta tatagaatta aaaaataata aattctgtat
    21721 aaatggagta acaggaccag atgaatatac agcaatagta aacaataatt actacactaa
    21781 tttaatggct aaatataatt taatatatgc agtacagatg gtagataaat ttaagcataa
    21841 tttattttca aatgaagtat ttagaaaagt taaattacaa gaatatgaag taaaagactg
    21901 gataaatgcc gctcaaaata tttatttacc atatgatgaa gagaagaaaa taagcaaaca
    21961 agacgattca tttttagaaa aatcagtatg ggattttaaa aatacaccaa aagaaaaata
    22021 tccattactt ctcaattatc atccattaac aatatataga tatcaagtaa ataaacaagc
    22081 agatactgta ctttcagcgt tgcttttccc tgatgagttt agcttagaac aaaagaagag
    22141 agattttgaa tactatgaaa aaataactac tcatgattca tctttgtcaa ggtcaatatt
    22201 tggaatgatg gcaagtgaaa ttggaaatca tgaaaaagct tataactatt ttatggacac
    22261 agcattaatg gatattaata acctacaaaa aaatacaagt gatggaatac atgctgctaa
    22321 tatgggtgga acatggatga gtttagtata tggatttgca ggaatgaaaa taaaaaagga
    22381 taaattaagt tttgaaccaa ggatacctaa acactggaaa aatattaaat ttaatatatt
    22441 atttagagat aatttagttt ctgtaaatat agaagaatat aaatcttatt ataagttaat
    22501 caaaggtaaa aacataagca tattacatga taataaagaa ataaaattat caaagaacat
    22561 aatagaagtt aataattgtt ttaaagggag taattaatat gaaagcagtc ttatttgatc
    22621 ttgatggagt tataacagat actgctaagt atcattttga agcttggcat gagatagcaa
    22681 aatcgctaga tatagaaata gatgaaactt ttaatgaaaa attaaaaggc ataagcaggg
    22741 aagaatcatt agataaaata ttgttatctg ctaataaagt agatagttat actaaagagg
    22801 aaaagttaaa ggtgtgtgaa gaaaaaaata acatttatat aaagttaata aactcattaa
    22861 catataaaga tatattacca gggataaatg agcttataaa agaacttaaa aaaaataatg
    22921 tgaaattggg aatagcatct gctagtaaga atgcacctag gatattagaa tctttgggaa
    22981 taaaagatga atttgattgt atagtaaatc catccttatt agaaagggga aaaccatatc
    23041 ctgatatttt tattgaaggg agcaagatgc taaatttaaa acaatatgat tgtgtaggaa
    23101 tagaagattc ttattcaggt attaaagcta taaatgctgc caatatgaca tcaattggtg
    23161 tggggaaaaa agaagtacta ggaaaatgtg accatattgt agaagatact agcttattaa
    23221 attacaatac actattacaa gcttggagta aaaagattaa tttggattaa aattatatta
    23281 aaaattattt taaaagagag gataaggtat gaagaataaa tatgaagaag tagctcaatt
    23341 gataattgat ggacttggag gaaaggataa tataattgac ttaactcatt gtatgacacg
    23401 attaaggttc atcttaaaag atgaaaagaa agtcaatgca gataaattaa aatctataga
    23461 taaggtagtt ggagtaaact caacttcaac tcaatatcag gtcataatag gtaatgaggt
    23521 aggtgttgta tttaatgcaa taataaataa ggggataaat accacaggag atattaaaag
    23581 cgatataaaa aatgaaaata aaaaaaatag tattttaaat aatataatag atacaataac
    23641 tggttgtatg acacctatga taccagccct tacagcatct gggatgataa aagttatatt
    23701 atctctttgt accactttta attggctatc atcaaattca agtacatata gagtattaga
    23761 tataatagga gatgtagcat tttactttat gcctataata cttgcaataa atgcagctaa
    23821 aaaatttaat gttaatacat caatagcagt tatagtagta ggtgtgtttt tacatccaaa
    23881 tttctctgca tgggtatcaa gtggggaccc tatatcattt ataggagtac caattcaagg
    23941 tgttatatat gcagcatctg ttatccctgc acttttaaca gtatggatga tgtcatatat
    24001 agaaaaattc attaataaat taacacctag catgttaaaa acaattttaa atccaacttt
    24061 agtattatta ataagtgcgc caatagcatt aatagtaatt ggaccaatag gaaatttctt
    24121 aggagaggga ttagctagta taataaattt actacaagga aggttaggat ttataatggt
    24181 ttgtttatta gcagctgcaa tgccatttat cgttatgaca gggatgcatc atgcattgac
    24241 tcctatattc atatcagcat ttgcaactac aggacaagaa tcgctaatat taatatctca
    24301 agtttgtgct aatttagcgc aaggtggcgc gacattagct gtagcattaa gaagcaaaga
    24361 tagtaagatg aaacagttag catcagctgc atggatatca gctatcatgg gtataactga
    24421 accagctctt tatggagtta cattgaaact taaaaaacct gctatgttag catctatagg
    24481 agcaggaatc gcagggttat ttgcaggaat tactcatgtg actttatatg ttccacaaaa
    24541 tagtataatg gcagtacttg gattatcagg ggaaaaggga atttcaaata tagtaaatgg
    24601 acttatagtt atatttatag cattggttgc tccatttatt atgacatatg tttttggagt
    24661 tgaaaaaaat gttaaggaaa gtacagataa tgtagtagag gaagtataat attaactatt
    24721 tacattaata gaatttttat tattattgac tataacttgt tttgtattta tattgatgca
    24781 aagaaataag ttaatattaa tgataataat tattataact gacaaaaagc aacacaaaag
    24841 attgatgaag tatatggaaa taaaaagttg acaaaatatg tctgatgttt tatcatatta
    24901 attggaatta ggtagtaatt gtttaagtac aagctaagct tattcattaa taatagcagt
    24961 atcctaggaa ggagtatgcc cattattatg gataagcttt ttttatgctt aaaaggagga
    25021 taatcatgat aattaataag atattaaaca ataatgttgt aatcacatta gatgataatg
    25081 atgaagaagt tatcgttatg ggtaaaggaa ttggttatca aaaatcaaaa ggcaatctaa
    25141 tagataaaac taaagtaaat aaagtattta gaatatcaaa taaagaaata tcaaacaaac
    25201 tacaagaatt actcaataat attcctatag aacacatgaa attatcaagt gaaattatag
    25261 aatacgcaca aataaagtta aacaagaagc taaatgaaag tatttacata tccttaagtg
    25321 accatacata ttctgcaatt caaagaatga aagaaggaat aaatgtaaaa aatgcaattc
    25381 tttgggaaat taaaagattt tataaagaag agtttgaaat tggaatgaaa gcactagata
    25441 ttattgaaaa caaaacaggt ataaaattac cagaagatga ggctggattt atagcctttc
    25501 atatagtgaa tgctcagtta agtgaaggtc atactttggc atctgatatt acgaagttaa
    25561 tacaagaagt acttagcata gttaggtatc attttaggat agaatttcat gaagaatcag
    25621 tgttttatta tagatttata atgcatttaa agttttttgc gcaaagattg cttttagata
    25681 gtgcacatga aggtgagaca gacaaagaat tactaagcat tattaaatct aaatataata
    25741 aagagtttga atgtgtagtt aaaattaaaa atttcattaa aaaacaatat aactacatat
    25801 taacagatga tgagcttata tatttaacaa tacatttagc taaagtagtt aaagactcaa
    25861 atatgtaagt gaaataattt ttcagtagta tattaagaat gattgaaagg agtgatacta
    25921 acaaaattat acctatggtt ggatggtaac attgagttgg ccaaaccttc aaatttcaaa
    25981 aaattaattt aaacgaaata tggaggagaa aaatgggaaa atacaaacaa ttagcacaag
    26041 agatagtaaa caatgttggt ggaaaagaaa atattagtgg attagtacac tgtattacaa
    26101 gattacgttt tactttaaaa gatgaaagta tagcaaatga caatgtatta aaaaacatgg
    26161 aaggtattgt tactgtaatg aaaagtggag gacaatatca agtagttata ggaaatcatg
    26221 ttgaagctgt ctataaagat gtagttgaaa taattggttt agatgattca agtacaagtt
    26281 cagaaactaa aaaatcaggt aatatactag ataaaggtat agatataata tcaggaattt
    26341 ttcagccagt gttaggaata atggcagctt gtggtatgct gaaaggattt aatgcattat
    26401 ttgtagcaat gggattatat agtgatgtat ctggtggtta tatggtaata aatgcagcag
    26461 gagatgcatt atttacattc cttcctttgt tcttaggata tacttcagct aagaagtttg
    26521 gattaaaacc aatgttggga ttagcactag gagctgcagt atgttatcct gctatacaag
    26581 gtagcagtat atcagcagga gcagatgtga tgtacacttt atttaaagga acaatgtttg
    26641 catcaccagt atatattgat ttctttggac taccaattgt atcaattgat tatactggaa
    26701 cagtcgtacc aattatattt atagtgtatt ttgcttctag gtgtgaaaaa ttatttaata
    26761 agtttgttcc agatttagtt aagtttttct ttgtgccaat gcttacatta ttaataacag
    26821 tgcctgttgc acttatagtt ataggtccta tcgcaacatt tggttctaca cttatttcac
    26881 aaattgtaat ttcaataaga gattttagtc cactattggc aggtgctata gtagggttta
    26941 cttggcaaat tcttgttatt tttggtatgc actggggatt tattcctgta tatataaaca
    27001 atgttatgac tttaggatat gataatgtta tgatgccatt ctttgcttgt acatttgcaa
    27061 gttcggcagt tgtgttggca atatttttca agactaaaga taaaaaacta aaagagatga
    27121 caataccaaa ctttatatct ggtatatttg gagttacaga gccagcaatt tatggaatat
    27181 tattgccttt aaagaaacca tttataataa gttgtattgc tagtgggatt ggaggcgctt
    27241 tctacggatt ctttaatctt agaaaattta taactggagg tatgggaata tttgaacttc
    27301 cagcaatgat tgaaccagat gggggaatgg gcaacttaat agtagctctt tctggtatag
    27361 ctatatcaat ggtcgtagct tttgtattaa ctatgatttt atataaagat gagaaagtag
    27421 aagagtctaa aaaagtaaga aataagggga aagaagaaat aaaagaagtc aagtctacta
    27481 aattagaaag agaaatagta gcaagtccta taaaaggtga agtattaaaa ttaagtgata
    27541 tagaggatgc agcatttgca tctggtgtac taggtcaagg ggtggcaata atacctagtg
    27601 atggaaaagt agttgctcca gtagatggtg tagttacaac cttatttcct agtctacatg
    27661 ctatagggat tctttctgat acaggtgttg aagtgcttat tcatattgga ttaaatacta
    27721 ttcaattaga agggagaggt tttgaggcat gtataaaaca aggtgataga attacaaaag
    27781 gtcaaactat attaaacttt gatgttgatg caataaaaga gttggggtat agtactgtaa
    27841 ctccaattgt tattacaaat tcatcacaat ttttagatgt agtagaaaca gaaagtaaaa
    27901 atattgaatt agaagataat ctaattacag tcttatttta gtaaaaattg aggcattagc
    27961 ataagctagt gctttggtta taatttaagg aggctttgac tatgagtttt aaggatgatt
    28021 ttttatgggg aggagctact gcagccaatc aatgtgaagg tggatacaat gaagacgaaa
    28081 gaggattggc aaatgtagat gtatgtccaa caggaaaaga tagaacagca gttattacag
    28141 ggaaactaaa aatgtttgat tttgatgatg agcattatta tccagctaaa actggtattg
    28201 atatgtatca taattataaa gaagatataa agttatttgc tgaaatggga tttaaagtat
    28261 atagaatgag cattgcatgg agtcgaatat ttccaaaagg tgatgaagaa acaccaaatg
    28321 aaaagggact tcaattttat gaaaatatat ttaaggagtg taaaaaatat aaaatagagc
    28381 ctttagtcac tattacacac tttgattgtc caatgcattt agttaaaaag tatggtgcat
    28441 ggcgtaatcg aaaaatgata ggtttctatg agaaattatg caatgttatc tttagaagat
    28501 atagaggatt agtgaagtac tggctaacct ttaatgaaat aaatatgatt ttacatgctc
    28561 catttatggg tgctggtatt tgctttgaag aaggtgaaga tgttgaaaag ataaaatatc
    28621 aagctgctca tcatgagctt gtagctagtg caattgcaac taaaatagca catgaagttg
    28681 attcaaataa cttagttgga tgtatgtttg cagctggaag tgtatatcct tatagttgta
    28741 atcctaatga tgtatgggaa gctacaaagt tagatagaga aaattacttc tttgtggatg
    28801 tacaatctaa aggtaaatat cctaactatg cgttaaagta tatggagcaa aaaggtatta
    28861 caccagaaat ggaacctggt gatattgaat tattgaataa atatacagta gattttattt
    28921 cattttcata ttacaatagc cgttgtgtta gaactgatga aaatgcagat gatatggctg
    28981 aaggaaacat atttacatct gctaaaaatc catatctttc atacagtcaa tggggatggc
    29041 caattgaccc attaggtttg agaattacac taaatcatgt atatgataga tatgaaaagc
    29101 cactatttat tgtagaaaat ggattaggtg ctaaagatat agctgatgaa aatggttatg
    29161 tagaagatga ttacagaatt aattatctaa gagaacatat aaaagctatg catgatgcag
    29221 tttctataga tggggtcgat ttattaggtt atactacatg ggcacctatt gatttagtaa
    29281 gtgctggtac aggtgaaatg gaaaagcgtt atggatttat atatgtagat agagataata
    29341 gtggtaatgg aagtctaaga agaatgaaaa agaaaagttt tgaatggtat aagaaagtaa
    29401 ttgcttcaaa tggtgaagat ttatattaaa caagaaatct atgttaaata agaaatttgt
    29461 attaaataag aagagtataa aaattaataa taagtataat ttttaacttt tttaactata
    29521 gaaaatcaat acagaattat tttacattgt atttatgcaa tagtatgatt cacaaataaa
    29581 attttagaaa ataaaattgt taagaagtat gggaagtaag tgcaatttac tttgtaataa
    29641 aagatgaaaa aggcgagctt aggttttaag cttgtctttt ttattatgta taaactatga
    29701 tactttaaaa ttctatgaaa aatttattta atagagaaat taaaaattta atggtaatca
    29761 aattctttac caatgaatat aaaaaatgtc tacgtgttat aatagtttaa actaataaac
    29821 gaaaggtaga tgaatgaata tgaaatacca acatataata gaagttgaca aagaactttg
    29881 cattggatgt gggctttgta aaaatgattg tccagtaaac aatataatta tagaaaataa
    29941 aaagtcagta attaaaaaac aagattgtct aatgtgtgga cattgtgcag caatatgtcc
    30001 cacaaaagct attaccttga caggatttga tgaacctcct atagaactta caaataagcc
    30061 aaagctagat tcagatgaat tactaatggc aattaaatct agaagaagta tacgtaaatt
    30121 taaagacaaa gaagtttctt cagaaattgt aaagcaaatt attgaggcag gtaggtatac
    30181 ccctagtgct aaaaattcac aagatgtatc atatattgtt ttagacaata aaaagtcaat
    30241 ttatgaaaac gaagcagtga aattttttag aaaaataaaa cctattgcaa atatagctat
    30301 taaatattca aaagaagttg agattgacga taactttttc tttaagcatg ctcctattgc
    30361 aataatgata attacaaaag ataagattag tggttcactt gcagcatcaa atatggcatt
    30421 aatggcagag tcttatggtc taggtgtttt atttagtgga tatttttcag atgtagcaaa
    30481 taattcgcca aaattaaaaa agcttttagg tctaaaacgc agtaatcatg ttcttacaac
    30541 attagtaatt ggatacccag atgttaaata tagacgtact gcacagaaag aagtagcaac
    30601 tgttagatac ttataagaga gggtggattt tatggaaaaa gaatgtcaac aaagaatgca
    30661 agaaataaca tctaaattta aagaatgtcg caaagctatc tctgctatgg gtgatgagac
    30721 tagacaattg attttaattg ctctcttaga gagtgatttt aatggtatac gagtgggaga
    30781 aatcacaaaa aaaacacatc tttctcgtcc tgctgtatct catcatctta agatattaaa
    30841 agaagcagag attgttaatt tgcgtagaga aggaacgaaa aattattatt atcttgattc
    30901 aaaaaaatca caatggaagt cactaacaga gctaataaat cttatatttg ttggaataca
    30961 acatattagt gaaaatgata taagaaaagg tgattaaatt atacttgatt taatacaaaa
    31021 agattgattt aggaactaaa aagttcactt tgagacacta tcttttgaac tatacccatc
    31081 ttaggagaca gttaaaatat gtaaaactgt ataaaacaat gggtatagtt cattttataa
    31141 tggtttaaat attaagaata tattattcta aatagtatct ttctctgttt tgagttttat
    31201 ttttatactc aatagcttca gtggggcaac cacaaataca tgccatacaa tgagtacagc
    31261 tatttttcca aactggcttt ttgtttttta aattaatatt atttaaagga catagctcta
    31321 cacattttcc acagccaatg catttatcag tataatgaaa tcctttggca ctaataattg
    31381 tcttataaaa aactgtattt attataccac ttttaattgt acctcctaag ccactaggag
    31441 taatagtttc aaattcattt tcattgcgta tatcagaaat aatcctatat atttgtttat
    31501 ctgcttcttt aatcatttgt tttgatgttt ctttgtctgt tgtagaaaat aaagctatat
    31561 aattctctgg cattttaatt tctgccatac cttttaattg ccattccttg tatttacata
    31621 gtttttgaat atagtttatg gattttgctg tatctccccc acaagtcatt acaaagtaaa
    31681 ctctgttgtc tcctagaaat tttgtttctt ttataaaatc tgtaaccact attggcaatc
    31741 tccatgcata agtaggacat acgaatatga atggtttacc tgctgatatt aattcatctg
    31801 ttttttcctc ttttataagt tgatttagtg atatcagttc atcatttaat tcttgtgcaa
    31861 ttttacgagc cacatatcgg ctattaccag taccgctaaa atataaaatc ataataatat
    31921 ctccctaact attttttatt ttaactctta attttttatt tattatagta ctttattgaa
    31981 atattctatt caaatgtatg taaaatttag tagttaagaa aattataaca cattagtaat
    32041 ttttatattc attggtaaag aatttgatta ccattaaatt tagtaacaaa atatcaactg
    32101 gatttttata taagaagaga tagtaatttt catgtgaaag attgtaaata taaagatgat
    32161 tttgaagggc aataagaaga taaaattgat agcttaataa aaaatattta acttttatat
    32221 taactgtttt aaatattata attatctatc ttgaat
//
LOCUS       DAGQWR010000031        32043 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.031, whole genome shotgun sequence.
ACCESSION   DAGQWR010000031 DAGQWR010000000
VERSION     DAGQWR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.031"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            48..365
                     /gene="flgB"
                     /locus_tag="MIW95_001231"
     CDS             48..365
                     /gene="flgB"
                     /locus_tag="MIW95_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBY3150573.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            373..777
                     /gene="flgC"
                     /locus_tag="MIW95_001232"
     CDS             373..777
                     /gene="flgC"
                     /locus_tag="MIW95_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBY3150574.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            787..1104
                     /gene="fliE"
                     /locus_tag="MIW95_001233"
     CDS             787..1104
                     /gene="fliE"
                     /locus_tag="MIW95_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBY3150575.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1129..2679
                     /gene="fliF"
                     /locus_tag="MIW95_001234"
     CDS             1129..2679
                     /gene="fliF"
                     /locus_tag="MIW95_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBY3150576.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAIPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDEDVDENNQPTNSLINVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKAEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2692..3762
                     /gene="fliG"
                     /locus_tag="MIW95_001235"
     CDS             2692..3762
                     /gene="fliG"
                     /locus_tag="MIW95_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBY3150577.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRDESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPALAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3755..4486
                     /locus_tag="MIW95_001236"
     CDS             3755..4486
                     /locus_tag="MIW95_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBY3150578.1"
                     /translation="MNNKIIKSKKASYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            4490..5809
                     /gene="fliI"
                     /locus_tag="MIW95_001237"
     CDS             4490..5809
                     /gene="fliI"
                     /locus_tag="MIW95_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBY3150579.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGIAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            5841..6287
                     /gene="fliJ"
                     /locus_tag="MIW95_001238"
     CDS             5841..6287
                     /gene="fliJ"
                     /locus_tag="MIW95_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBY3150580.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIESNLE
                     NLESNYSKYSISKNNDSVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6315..7520
                     /locus_tag="MIW95_001239"
     CDS             6315..7520
                     /locus_tag="MIW95_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBY3150581.1"
                     /translation="MKIQADSYNIIKDIGNRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRRETSKQNVSSDKRENSDNECHLDEDKSNHLEDENTYEKDCSNNNEALHQLLNLLK
                     DNYKKDKDINTILNNLKKLSISDELKQELKLNKNILNKIANQNLELNRASTNNPNDIE
                     NLDIMKLRELLENNASEDIEVISKTTENDTKSIDLYSFDSNRIDTLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            7538..8146
                     /locus_tag="MIW95_001240"
     CDS             7538..8146
                     /locus_tag="MIW95_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBY3150582.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEEEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDANSGEENDSSEQESPEEV"
     gene            8173..9156
                     /locus_tag="MIW95_001241"
     CDS             8173..9156
                     /locus_tag="MIW95_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBY3150583.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGVIYDGEYKADT
                     GEFIKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GNIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9169..9372
                     /locus_tag="MIW95_001242"
     CDS             9169..9372
                     /locus_tag="MIW95_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBY3150584.1"
                     /translation="MIRVTDLNGRRYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKVNVGYIRSEK"
     gene            9372..10190
                     /locus_tag="MIW95_001243"
     CDS             9372..10190
                     /locus_tag="MIW95_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBY3150585.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEVNEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10180..10875
                     /locus_tag="MIW95_001244"
     CDS             10180..10875
                     /locus_tag="MIW95_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBY3150586.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGISMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKADSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            10887..11330
                     /locus_tag="MIW95_001245"
     CDS             10887..11330
                     /locus_tag="MIW95_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBY3150587.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11345..11719
                     /locus_tag="MIW95_001246"
     CDS             11345..11719
                     /locus_tag="MIW95_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliO/MopB family protein"
                     /protein_id="HBY3150588.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMNKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11720..12385
                     /gene="fliP"
                     /locus_tag="MIW95_001247"
     CDS             11720..12385
                     /gene="fliP"
                     /locus_tag="MIW95_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBY3150589.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12398..12667
                     /gene="fliQ"
                     /locus_tag="MIW95_001248"
     CDS             12398..12667
                     /gene="fliQ"
                     /locus_tag="MIW95_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBY3150590.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            12679..14493
                     /locus_tag="MIW95_001249"
     CDS             12679..14493
                     /locus_tag="MIW95_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBY3150591.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTARMIFFSMDGHHVLIRGIRYSFELIPIGKSFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVLKR"
     gene            14502..16577
                     /gene="flhA"
                     /locus_tag="MIW95_001250"
     CDS             14502..16577
                     /gene="flhA"
                     /locus_tag="MIW95_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBY3150592.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVSKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAEIKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16552..17568
                     /gene="flhF"
                     /locus_tag="MIW95_001251"
     CDS             16552..17568
                     /gene="flhF"
                     /locus_tag="MIW95_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBY3150593.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKLIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVVNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17565..18443
                     /locus_tag="MIW95_001252"
     CDS             17565..18443
                     /locus_tag="MIW95_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBY3150594.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18457..19158
                     /locus_tag="MIW95_001253"
     CDS             18457..19158
                     /locus_tag="MIW95_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBY3150595.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELNVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19174..19596
                     /locus_tag="MIW95_001254"
     CDS             19174..19596
                     /locus_tag="MIW95_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBY3150596.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            19581..19766
                     /locus_tag="MIW95_001255"
     CDS             19581..19766
                     /locus_tag="MIW95_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBY3150597.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19807..20580
                     /gene="flgG"
                     /locus_tag="MIW95_001256"
     CDS             19807..20580
                     /gene="flgG"
                     /locus_tag="MIW95_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBY3150598.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNDYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20601..21362
                     /gene="flgG"
                     /locus_tag="MIW95_001257"
     CDS             20601..21362
                     /gene="flgG"
                     /locus_tag="MIW95_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBY3150599.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRIYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNIDGLGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21385..22266
                     /locus_tag="MIW95_001258"
     CDS             21385..22266
                     /locus_tag="MIW95_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBY3150600.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMIVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVIKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22269..22643
                     /locus_tag="MIW95_001259"
     CDS             22269..22643
                     /locus_tag="MIW95_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBY3150601.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELNTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22675..24078
                     /locus_tag="MIW95_001260"
     CDS             22675..24078
                     /locus_tag="MIW95_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBY3150602.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNVKVDDYKNEFLHKSSSNHKIND
                     YNNVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEADVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24194..26131
                     /gene="htpG"
                     /locus_tag="MIW95_001261"
     CDS             24194..26131
                     /gene="htpG"
                     /locus_tag="MIW95_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBY3150603.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITIQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            26519..27649
                     /locus_tag="MIW95_001262"
     CDS             26519..27649
                     /locus_tag="MIW95_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBY3150604.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDIDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            27876..28898
                     /gene="splB"
                     /locus_tag="MIW95_001263"
     CDS             27876..28898
                     /gene="splB"
                     /locus_tag="MIW95_001263"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBY3150605.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29080..29991
                     /locus_tag="MIW95_001264"
     CDS             29080..29991
                     /locus_tag="MIW95_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY3150606.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYNTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30006..30422
                     /locus_tag="MIW95_001265"
     CDS             30006..30422
                     /locus_tag="MIW95_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBY3150607.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(30516..30908)
                     /locus_tag="MIW95_001266"
     CDS             complement(30516..30908)
                     /locus_tag="MIW95_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3150608.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31049..31444
                     /locus_tag="MIW95_001267"
     CDS             31049..31444
                     /locus_tag="MIW95_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBY3150609.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            31847..>32043
                     /locus_tag="MIW95_001268"
     CDS             31847..>32043
                     /locus_tag="MIW95_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150610.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFN"
BASE COUNT        13146 a         3387 c         5902 g         9608 t
ORIGIN      
        1 ttattaataa gcaaaataaa taaaatatat tttgaggagg agtgagtgtg aatacatatg
       61 acattataaa aatgggatta gatgcaacta atttaagaag tcagacaata gcaaataata
      121 tagccaatat aaatactgaa ggatataaga gacattatgt aacttttgaa gaaacattaa
      181 atgatgctat gccaaaaata gaagaaaaaa cagacaattc taaaggatta agagtggatg
      241 gcaataatgt agatatagaa gaagaaaaag taaatcaagc aatggcaaca ttacaatata
      301 atgctttgat aagttttgca aatggaaaaa tagcaatgac taaatcaata ataagtggga
      361 ggtaattaaa aggtgagtgt gttcagtgga atgagaatat ctgctagtgg attaacagca
      421 gaaagcttga aaatggatat catatcgtcc aatgtagcta atataaatgc cacaagaggt
      481 ccaaatggag aagcatatag gagaaaagtt gtttcatttg aagaaaacta tgataataaa
      541 ttaggtatgc taggagtaaa agttacatct gttgaagatg ataaatcacc tcttaaagaa
      601 gtatatgacc catctcatcc agatgcaaag ccaaatggat atgtagaata tccaaatgta
      661 aatatattgg tagaaatgac agatttaata acagcttcac gagcatatga ttctaacgta
      721 gatacattga atgctcagaa gtctatgata tcaaaagctt tggaaatagg aaagtaggga
      781 taaattatgc caattacaca aattaacaat ttaatgcaag gtgcaaagtt agataatttg
      841 attggagaaa caaaaccatt aactcttaat aataaagaag aaaaaaagaa ctttgaagat
      901 gtaataatag atactataga taaagttaat tttcaacaag tgacagcaga tgatatgaaa
      961 gaaaaattta taaaaggcga agatgtttct atgcatgaag ttatgttaat aggtcaagaa
     1021 gcacagctat ctatgcaata tttaatagaa gttagaaata agattcatga tgcatatcaa
     1081 gaaatgaata aggtacaaat ttagcctttt gaacaggagt aataatcaat gatagccagt
     1141 tttaaaaata agattaaaga gcaagttgct aaatctagga caaagataag ttctctaaaa
     1201 aagggaacta aaatagcatt aggtataggg gttattgcta tatgtttagc gctagtattt
     1261 acagcaatat ctgcgagcaa aaataagtat gcagtcttat tttcagaact agatgttcaa
     1321 gatgctgcag atatatcagc agaattagag tctaaaaaga tagatactaa ggtaaaggga
     1381 agtacgattt atgtacctag aacagaagtt gataagttaa ggctggagct agcaccaagt
     1441 cttacaggtg gttctaaagg atttgcacta atggatgata gttcatcaat aggacttact
     1501 gatgaagagt ttcagattaa gaagcaaaga atgatacaag gtgaaataga aaaaacaata
     1561 aagagttttc cacaagtgga agatactaga gtacatataa catttggaga gtcatcagta
     1621 tttgagaagg aggctattcc agggagtgca gcagtttatt tgatgttaaa accagggacg
     1681 actttggaaa aaaaccaaat aaaatctgtt atggcactta tatcaggaag tgttacaaat
     1741 ataccagaga aaaatgttca agtcattgat aacaaaatga atttattatc tgaaggtatt
     1801 ttagaggatg aagatgttga tgaaaataat caaccaacaa atagtttgat taatgttgat
     1861 aaaaataagg catctgaaca agcattgaat aaacaattag aaaagtctat tttagaaatg
     1921 ctagaaccaa tatttggtaa aggaaaagta aaagccacag taaatgctga tttaagtttt
     1981 gatacagctg aaaaaactga gataaaaatt gaccctgata aagttgcaat aaaggagaca
     2041 aggtcgaaaa attcatctaa atctgctaca aataatgttc aaggtgtaga tgctaacatg
     2101 aataaccaag gaaataacaa tggtactaat acagaagata gtttagatga gagttttgag
     2161 tatgaaacag gtaagattga aactcacact atagttgcgc ctggagaact taatagattg
     2221 acagcttctg ttgcaataga tggaaaaata gctaaaggtg tacaagctga tgtagaggat
     2281 attgttaata atgctatagg tatggatgga gcgaggggag atactcttag cgttgtttca
     2341 atggtatttg acccagaagg gaaggcagaa gctaaacaag agctagaaga gattaaaaaa
     2401 gaaggattta aaaagaacat aataaaagca gttattggag tagtggcagt ggtagcaatt
     2461 ggagctattg gatacttaat atataagaaa agaagtgaac gagaagatat ggattatgaa
     2521 gatgaagatt atatttctaa atctgattct gttgatagga aggataatcc tctattagga
     2581 gatgacccag aactgacatt agaggaagct gttaaaatgt atgcagaaga aaagccagac
     2641 caagtaacag agataataaa aacttggtta aatgagtaag ggggatagaa agtgtccacg
     2701 gaaattacaa atgttaatgc gtttgactta agtgatataa atgaaagtga taagaaagag
     2761 aagatagaag gtgagggttt aaaaaaagct gctgtattat taatgacatt aggaccaaat
     2821 gtgtctagtg atataataaa aagcttgcct gataaacaag ttcaaaaaat aggtttagaa
     2881 attgcaaata tttcttcaat agcttcaaaa gaaagaagag aaatcttaaa agagtttgta
     2941 gagataaata gggcaaaaga ttatgttata gaaggtgggc ttgaatatgc aagagagttg
     3001 ttaagcaatg ctttaggaaa tgctaaagca aacaaattac ttgaaggtat atcaataaat
     3061 acaagtacaa agccttttaa acatattata aaactagatg taaagaaaat attagaaata
     3121 cttagggatg aaagtgcaca aactatagct gttttacttg cacatatgga gccagaaaat
     3181 ggagcagaag ttttatcata cttagatttt gatttacaaa atgaagtagc tatgagaata
     3241 agctcaatgg gttcaatatc tccagaagta ataaaggtaa ttgaaagttc attagaagat
     3301 aaattacact caataagtgg tgatgatttt aaggattatg gaggagtgtc taccttagtt
     3361 caaatattga ttaattctga tagaaaaaca gaaaagaata ttttgggaac tattgaagaa
     3421 caagacccgg ctttagcagc tcaaattaaa tctcatatat ttgtctttga agatattgtc
     3481 aagttagatg atataagtat acaaaaagta ttaaaagaag tgactatgaa agatttagta
     3541 tatgccttaa agggtgtgga tgatgagatt tcagatgcta tatatagaaa tcaatcttct
     3601 agagcagcag atgcacttaa agaagaaatg gaaatggttg gtgtcataaa agttacacag
     3661 gttgaggctg ctcaacaaaa gatagcaaat atagtaagaa aacttgaaag agaaggtgcc
     3721 ataactattg taaggaattt tggtggtgac tttgttgaat aataagatta ttaaaagtaa
     3781 aaaagcatca tataaaggag tgctagattt aaccataata gataaagtta gagtgggaga
     3841 atcaaatgta aataaagaaa atgattgtag atttagagat gaggtaataa aagaagctat
     3901 agaatctgct gagataaaaa agaatgagat attagaagaa gcaaaaatga atgcacaaaa
     3961 tatagaaaaa caagcatatg aaaaagggta tagtcaaggt caaaaaaatg gatatgaaga
     4021 tggatataaa gagtcatatg atgaatatac ccaaaaagct aaagatgaag cagattttat
     4081 aaaaaatcag gcagacttaa tactattaca agctaatgag aagatgactg agtacataaa
     4141 agaaaaaaat aaagagattg taagacttgc tattaatatg gctagttctg ttctaaaaaa
     4201 gcatttcgaa gatgaagaat ctatgagtga tttgttaaag caaattattc aagattatga
     4261 aggaagtagt tcatttatag taagatgtaa ttctttctat aaagataata tagagaaaga
     4321 gtttgaatta ttcaaagagg aaacatcact aaaatcaagt gtatttgtta ttgcagatga
     4381 tagtatagat aaaggcaatg ctatcatcca aaaggaaaat ggaagaataa tagttggtat
     4441 cgattgtgct atggaaaaac taaaagaaga attgcttggt gaataaagta tgtataaaac
     4501 tgatatatct atagattttg acaaaataaa taagaaaata gaaaatttag aactcactat
     4561 atcagaaggt agagtaaaga agataatagg acttactgta gaagttgaag gaataaaagc
     4621 ttttgttggg gagttatgtg ttatatacaa tcaagtcaat aaaccagtga attgtgaagt
     4681 agtgggtttt aaagataaag aagtcatatt aatggcgttg ggagagctaa cattgatagc
     4741 tcctggttgt aaagttattt ctaagggaat acctttaagt gttatgtgta gcgataattt
     4801 actaggtaaa gtacttgatg gtcttggaaa tcctatagac aactcagatg ctgttttagg
     4861 agatagatac aacttgaaca atgaacctcc tgacccaatg aagagaaaaa agatacgaaa
     4921 tataatggaa acaggtgtaa gagcaataga tgcatttact acttgtggtg aaggacagag
     4981 aatagggata tttgcaggaa gtggagtagg taagagtacg actctaggta tgattgctag
     5041 gaatgcaaaa gcagatgtaa atgtaattgc ccttattgga gaaagaggaa gagaggtact
     5101 agattttatt gataaagatt taggtgaaga gggaatgaaa aaatctgttg ttgtatgtgc
     5161 aacatcagat aaagctcctt tggttaggct aaaaggtgct cttactgcta ctgcaatagc
     5221 agagtatttt agagaccagg gaaaaaaagt aatacttatg atggattcag taactagatt
     5281 tgctatggca caaagagaag ttggtatagc aataggagaa ccaccagctc aaaaaggata
     5341 tacaccatct gtatttgcta ttttaccaaa acttatggag agaacaggga catcagataa
     5401 aggttctata actgcatttt atacagtatt agttgatgga gatgacttta atgagccaat
     5461 agcagataca acaagaggga tacttgatgg tcatatagtt ttatctagag acttggcaaa
     5521 taagaatcat tatccttcta tagatgtatt gaacagttta agcagactta tgaatgagat
     5581 tgcttcaaaa gaagacataa aaatagcatc ttttgcgaga gatatgcttg ctgagtatag
     5641 agaagcagaa gatttgataa atataggtgc atatgcttca ggtacaaaca aaaaaataga
     5701 tgaagcaatc tactatcatg agcatattat taatttttta aaacaaggaa taaatgaaaa
     5761 gagttctttt aatgaaacta taagtagttt aagaagaatc tttgaataaa caatttaaaa
     5821 aaatatttga ataggttgtg atggaattgg ataaaaattt taagtttaga ttacagaaag
     5881 ttttagactt aaaaataaaa gatgaagaag agataaaaat ggaatttgct aagattcaac
     5941 aaaaaaaaat agatatagag agcaatttag aaaacttaga aagtaattac agcaaatatt
     6001 ctataagtaa aaataatgat agtgttcaaa atcaaaaaat aactattaac tatttgttag
     6061 ctttaaacaa ctctataatg gatttatcag aagagctgga taagtcgaca aatgaacttg
     6121 aaaaagctag aaagcagctt ataagcaaac agatagagag aaagtcatta gaaaaattaa
     6181 aagaaaaaaa atatggacag tattataaag aagaacagtt aaaagaacaa agcactaatg
     6241 atgagtttgc aagcatgagt tatttgagaa atagacaagt tctataaaat agtaggaagt
     6301 gaggatatta ttgtatgaag atacaagcag attcttataa tattattaaa gatattggga
     6361 atagaaaaag ctcatctaag aataaaaata taaaaaataa taaatttgaa gataatctat
     6421 ataaggcaac agaccataaa aaggttaaag atagaagaga aacaagtaag caaaatgtta
     6481 gttcagataa acgggaaaat agtgataatg agtgtcattt agatgaagat aaaagtaatc
     6541 acttagaaga tgaaaataca tatgaaaaag attgtagtaa taataatgaa gctttacatc
     6601 aattactgaa tcttttaaaa gataattaca aaaaagataa agatataaac actatattga
     6661 ataatctaaa aaaactaagt atatcagatg aattaaaaca agaacttaag cttaataaaa
     6721 atatattaaa taagattgca aatcaaaatt tagagttaaa tagagctagt acaaataacc
     6781 ctaatgatat agaaaattta gacataatga agttaagaga acttttagaa aataatgcta
     6841 gtgaagatat tgaagtaatt agtaaaacta cagaaaatga tactaaaagc atagacttgt
     6901 acagttttga cagcaataga atagatactc ttaactcaaa taaacagaaa gatgatagtt
     6961 ctgatatttt ggaaaaacta gcaggggtaa atggaaataa gagtggaaat tttggacaag
     7021 taataaataa aagcgttgat atgaataaaa ttaaaaatga taatgttaat ttaaaacaga
     7081 tagaatcaaa tgtaatggat gattctataa aagctattaa gcatatgaaa acaaatgata
     7141 tacaagaatt gacaattaaa ttaagaccaa aagaattagg agatatgaac atacaattgt
     7201 taaaagatgg tgaaagtatg agagctgttg tgactgtttt caacaaagat gtctttgact
     7261 ctataaataa aaacatagca gatttaaagc aacatcttga acttacaaat gtaaatataa
     7321 aagatgtatc agtgcaaatg catagcgata atacaaatac atctgatacc tttgataaag
     7381 catttgaaca acagaataga caaaataacc aagaaaacat gagtaagcaa aatacaaatg
     7441 ataagcgtaa caatgtagtc atagaagatg agattaaaga aaatattatt gatgatgata
     7501 gagtagattt actagcttag taagggggaa gtattgcatg tacggaatag caacggcttt
     7561 ggcaggaact caggtaaact ataggtcaaa taatttaagc caacttgaaa gaaatattaa
     7621 tgatattgcc aataaacaag ttagaagtaa aggaaatcag acagtaacag acaatgggac
     7681 tccaattata ggacaaggcg atgatgctga taaagatttg tttatgaaga ttcttgtagc
     7741 acagatgggt aatcaagacc caatgaatcc acaagaccca acagaatatg tatcacaatt
     7801 agcacagttt gcaactttag aaaagatgtc agctatgaat gataagctag atatactttt
     7861 agccttaagc aattccactc taataaattc tgctttgagt atggcaacat cattaattgg
     7921 taaagaagta gaagtatctg gggaagagga agaagatgaa aacattgtag gagtagtaaa
     7981 atccacatat ataaaagatg gggaagtaca cttagaatta gaagtagatg gaagtgacaa
     8041 aactgtaggt gttaaatatg aaaaattact taaaatagga gaggtagatg caaattcagg
     8101 agaagaaaat gactcttcag aacaagaatc tccagaagaa gtataatatt aaatgagaaa
     8161 agaggtaaat ttatgataaa agcaatgtat tcaggtgtaa gtggaatgaa agcaaatcaa
     8221 acaaaactag atgtaatagg taacaatgta gccaacgctg gaactacttc ttttaagaaa
     8281 agttcagcta gaataacaga ttcattttat caaactgtac tatatgcaag tgctccaaca
     8341 gcggcattag ggggaacaaa cttaggacaa gtaggtgtag gtagtaagat atcaagtata
     8401 aataaagata tgacacaagg aaatgtacaa ccaactaata ggccatctga tttaatgata
     8461 gatggagatg gatatcttcc agtagtaaga caaggaacag ttatgtatac gagagatggt
     8521 tcttttaatt tagatacagg atttaaagca gctgatggtg ctttgaagaa tattaaaggt
     8581 ggaagacttg taaatggtga tggatatatg ttacaaggtg tgatttatga tggggaatat
     8641 aaagctgata ctggagaatt cattaaaaag ggtgatgcta aaggaaaaga gcctattgta
     8701 ataccacttc aaagtataag tcctaaggat ggaacagttc aaaatgtact agagtataat
     8761 atatcaaagg atggaacagt agaattttta ttgagtgatg gtcaaagaac tcaatttgtc
     8821 tatgatatac ctgatggtgc taaggtagga gatacacctg ctggaataga taaattaaaa
     8881 ggtacaatac aaaaagtgca aacatatgca ttccaaaatc cagcaggact agatgcagaa
     8941 ggaggaaact tattcaagcc atcagcaaac tcaggagatc cacaaatagc aggagctaca
     9001 ggaaacataa tacaaggggc aatagaagct tccaatgtag atatagcaga agaatttaca
     9061 gaaatgataa tagcaagtag gtctttccaa gcaaacagta aaataatatc gacatcagac
     9121 gaaatacttc aagaaataat aaatcttaag agatagtagg tacatattat gataagagta
     9181 acggatttaa acggaagaag atacatatta aattcagacc ttatagtgag aattgactct
     9241 gtaccagaaa gtaggattct ccttacaaca ggagaaaaaa atcttgtaaa agagtcagta
     9301 gatgagatag tagataaagt aataaagtat aaaagaaaag taaatgtagg ttatattagg
     9361 agtgaaaaat aatgaaaaaa acagattcac taacattcat tggactaata ttatctacag
     9421 tattagtttt atttggggca gtaaaaggaa gtagttcagg attgaaaagt tttttcgatt
     9481 ttgcatctat attaatcaca gtatttgggt catttggagc tttgatgatt acatttacta
     9541 ttgatgatat aaaacttata aaagatgcgc tacaatactc attcaagaca atgaatatat
     9601 cgaaattaga cttattaaat caatttaaaa cactttctaa aaaagcaaga agagaaggac
     9661 ttttatctat agaaagtgat gtaatgaaaa tacaagaccc atttatgaaa aaagggttag
     9721 agttatgtat tgatggcctt gaagtaaatg aaataaggtc tatactagaa ctcgaaatag
     9781 attctgttga agaaaccaat aacagatatt ctaaactatt taaaacttgg gggactttcg
     9841 cacctgcatt tggtatgttg ggaactctaa taggtttaat acaaatgtta tctgacctta
     9901 catctccaga tttaatagca tcgggtatgg gaaaagcatt aattacgaca ttttatggtt
     9961 ctttacttgc taatatcgct ttgaacccaa ttgcatataa tatagatgag aagactgaaa
    10021 aagagattta tgtaaaggaa atgatgctgg aaggtataat aagcatacaa tctggggaga
    10081 gcagcatagt agttgaggaa cgtttagcta catacctttc aaacaatgag aagctagaaa
    10141 ttatgaaatc caataaaaat acagaaagag caatgagtaa tggcgcgtaa aaaaaataag
    10201 aaaaaggatg agataaatcc agatgcatgg ttggctactt atgcagatac tataacactg
    10261 attcttacat tttttgtgtt attgtatgct acatcagttg tagatatgga aaaatttaaa
    10321 aacatagcag atgcgttaaa aggtcagttt tctgggataa gtatgttcag tacaggttca
    10381 agtacaagtg agaagtctcc aatagagagt gttttagtgg ataaaaatga taagtcaaat
    10441 ttagaacagg atttaaaaga taaagttaat aattctaact tacaaaatag tgtaacagtc
    10501 aaagatgata gtagagggat tttactagaa ctagatgatt caattttatt tgactctggg
    10561 atttctgaat taaaagcaga tagtaaagcc actcttaata aagtatatga catgataaag
    10621 atgatgaaaa ataagatagt tatagaaggc catactgata atgtacctgt aaaatcaagt
    10681 acacatgaat ccaattggga tttatcttct agtagagcag taagtgttgt aagatacttt
    10741 gtagaagaaa agggcatgga ccctagacta ttttcggcaa caggatgtgg agagtatcaa
    10801 cctttggttt caaatgaaac agcaaaggga agggctgaga atagaagagt taatatactt
    10861 ataactattg actaggagga agaggtttgt tcaaaaataa aaaaattatt ataattattt
    10921 tgatactgat tattttctca gcaggagcag gtttttttat attaggagaa ggtggaaata
    10981 aacaaattga caagattgca ggtttgttta ttccaacagg gaaaggcaaa gatgaagtaa
    11041 agaccgaaaa tcttgactta gaagagatgg tattaaagct agctgatgaa ggtgtaagat
    11101 atttaaaagt atcaataact gtatcttatg attcttcata taaagatata gaaaaaaata
    11161 cgcctttaat tagggataat attataaatt gttttatggc aaaaagggca gatgatttta
    11221 ctgctgataa tttaaagaac ataaaagaag aaattaaaaa tacattaaat atacaactag
    11281 gtgaaaatgt aattcaaaat atatatttca caaatatagt agtgcaataa aaggatggta
    11341 agacatgata ctttatttac taaaaatatt aatatgtatc ccaataattt taatattgat
    11401 agtcttatct ttaaaaatta gccaaggaac tttattaaaa aaatataatg ataaatatat
    11461 aaaaatacta gatattgtaa atataagtaa aaacaatagc ataataattt taaaaatagg
    11521 tgaagaagga tgcattatgg catcttcttc ctctagcatg aataaaataa aagatttgac
    11581 caaagaagaa atattaagaa tagaaaacga cattaaagaa agaaatttta aaattcaaga
    11641 ttttaaaatg gaaagaatta aaaagaaata tgatttcagt agatatattg gaaaatttac
    11701 aaaaaaggaa gataagtaaa tggaactatt atcaaaatta cctttggctg atgtaccata
    11761 tacaagttca atgcaattgt ttttatttct aactgcatta atgtttttac catttgtaat
    11821 gtttatgatg acaagctttg tcagaatagt tataagttta tcatttttga aatcagcatt
    11881 aggagcacag caagcaattc caagccaaat tttggtaggg cttgcaatag tattgacaat
    11941 atttataatg agaccagtat taaatgagat aaacgaaaag gcattacaac catatatgaa
    12001 agaagaagtt actatggaag aagctatgaa ggaagcagag ggacctatta aagaattttt
    12061 attaaagcaa accagacaga cagatttaga tttatttgta gaacaggctg gtttgaaaga
    12121 aaagaaatta actcgtgaaa atataccact atctgttgta gttccagcat ttgctataag
    12181 tgaactaaaa actgcttttc aaataggttt tttaatatat ataccatttt tgataataga
    12241 ccttgttgtg gcaagtgttt tgatgtctat gggtatgttt atgttgcctc cagtcatgat
    12301 atcattaccg tttaagttat tattatttgt aatggtggat ggatggaact tgatagtaaa
    12361 aacattgata ttgggatttg gataggagaa aaatagtatg agtgaattta tgataatgag
    12421 tgttcttaaa aatggcatgt ttacaggagc tatgattgca gctccagtac tcattgtttc
    12481 tcttgtagtt gggcttttaa taagtgtgtt tcaagctaca actcaaatcc aagagcaaac
    12541 tttgactttt gtaccaaaac taatagcaat tcttttagta gtagctattg gtggaagctg
    12601 gatgttacat actcttatta gctttacttc ggagatattc aatatgatgt ctcttataac
    12661 taaataagta ggatatatat gatttttgta tttattagaa tagtggcatt ctttggaaca
    12721 ctaaggatac tatttccagc tggtacacct gctttattta agtcgctctt tgcaatcatt
    12781 atatcaatat tgatttcaag tactatgcaa attgaatatg caacaaatat agacaacatt
    12841 gtattgttta ccttatatgg tataaatgag accataactg gcattatgct tggatatata
    12901 accaatctat gtttttatag tatcagaatg gcaggttcta tgatggatca acagatggga
    12961 ttatcaatga ttaatatgtt tgacccaaat tcaatgactc agacaacttt aattgataat
    13021 cttatgaact ggacagcacg tatgatattt tttagcatgg atggtcatca tgttttaatt
    13081 agaggcataa ggtatagttt tgagttaatt ccaataggga aatcattcgt agataataat
    13141 atagattata taataaacat atttgtgcag tgctttttaa ctggattcaa aatagcaata
    13201 cctattgtac tttgtctgct tatggcagat tttatattag ggcttatatc aagaagtgta
    13261 cctcaactaa atgtaatgat aattggtatg ccattaaaaa tactggttgg tattgcgtta
    13321 tttatcatat caataccttt aattgtcaat caaataagtc atttattaag tcaaatacca
    13381 aagatgtatg agggaacttt cgcactagct cctatgtttt ttatgggttc aacagataaa
    13441 acagaagagg ctactccaaa gaaaaaggga gaacaaagaa aaaaaggtaa tatagcaaag
    13501 agtaaagaat tacccgttgc aatgactcta cttgcattca ctttattagt acctacatta
    13561 ttttcatatg tagtggatac acttaaatct tctcttaatt attttttgag tctagatttt
    13621 tatatgaata taaattattc aagtatagag aaattagtta tagcaggatt aatggatttt
    13681 tttaagatat ttttaccaat agctattcca ttcttagttt taggtatagt cgcaaaccta
    13741 tttcaagtgg gaatactgtt tactggtgaa actttaaaac ccaatttatc aaaattaaat
    13801 ccagttagtg gatttaaaaa catgttttct atgagaagtt tgtcaacctt aataaaggat
    13861 acagcgatta tatctatttt ggcttacata ggatatacat tcttccaaga taattattta
    13921 gatatattaa agctaggtaa catatacctg ccaactttaa tgtacacagt taaagattta
    13981 gtatattcaa tattaagtaa gatttgtgta gctatgatag ttatagcagt tgcagattat
    14041 gtttatcaaa gatacagtca taaaaagcaa cttagaatga caaaacaaga agttaaagat
    14101 gaatataaaa actcagaagg agacccagaa ataaaagcta agataaaaca aaaacaaaga
    14161 caaatttcgt ctcaaagaac catgcaagca gtaccaagtg ctactgtaat agtaaccaat
    14221 cccacccact tatctatagc agtgagatat gaaaaaggga aagaccaagc tccagtagtg
    14281 gtggcaaaag gtgcagatta tttagctttt aaaataaggg aaattgcaaa gggaaatgac
    14341 atacctataa tagaaaataa acctattgct aggttgttat ataagcaagt agaaatagac
    14401 caagaaattc cagaggacat gtatcaagct tttgcagaga tattagtagc agtatataaa
    14461 atcaaaaata gatacaaagt cctcaaaagg taggataagt aatgaataag tttaatttaa
    14521 aggaaaatat ggacgtaata gtggcatttg gtgttgtagg gattgttctt atgatgatag
    14581 tgccgttacc tacatttata ttagatattt tactggcaat taacataagt ttatctgtgc
    14641 taattttact aatgacatta tttacaacaa atgtacttga tttatctata tttccaacag
    14701 tgttacttgt gacaacacta tttagattgg gattaaatct ttcatcaact agattgattt
    14761 taacacaggg atatgcaggt gaagttatag aggcatttgg aagttttgtt gtaggtggaa
    14821 attacgtagt aggaataatt atattcttaa ttatattaat tgttcaattt gttgttataa
    14881 ccaatggttc tggaagagta tctgaagtaa cagctagatt tacgttagat gccatgccag
    14941 gtaagcagat gagtatagat gcagatttaa atgcaggagt tatagatgac aaaactgcta
    15001 gaaaaagaag aaaagagctt caacaagaag cagattttta tggctctatg gatggagcaa
    15061 ataagtttgt aaagggagat gccatagcag gtcttatagt aacagcaata aacataatag
    15121 gtggaattgt aataggagct gtaatgctga atatgactct tatgggtgca gcacagacat
    15181 atgtaagact tactataggg gatggtcttg taagccagat acctgctcta ttaatatcta
    15241 catcagcggg tataatcgta acaagagctt ctactgatga aaactttggt ggattagtaa
    15301 gtaaacagtt aacggcaata ccaaaagcag taatgatgac tggttgtgtt ctaatcgtac
    15361 ttggtatgat gcctggatta cctaaaccag catttttttc actaggtata ggtgctatag
    15421 cagcaggtta ctttttagga agagaaaaga aagatacttt agaagacttg gagtttgaaa
    15481 aacctgatat ggtaaatcca gataaaaatg aagtagaaga tgtaacaaac ttaataaatg
    15541 tagaatcaat agaagtagaa attggatatg gtttaatatc tttagctgat gaatcagcag
    15601 gaggagattt acttcagaga atagcatcaa taagaagaca atgtgcaata gacatgggta
    15661 tagtcgtgca acccataaga ataagagata atttacagtt aaatccaaac caatacaata
    15721 taaaaataaa gggaaatgta gttggtgggt atgagataat gcctaatatg gttttatgta
    15781 tggaccctat gaatcaagga tttagtatat ttggaataaa gacaattgag cctacttttg
    15841 gaatagaggc actttggata gaaagtgaca aggtagaaga tgcagagata aagggttata
    15901 cagttgtaga ttcatccaca atacttataa ctcacttact agatataata aaatcaaatg
    15961 cacacgaatt actgggaaga caagaagtta agactattat agatgcagca agagaaaatt
    16021 atagtgctgt agtagatgaa ttgataccag atttaatgac acttggtgaa attcaaaaag
    16081 tacttaaaaa tctgttaaga gaaaaagtaa atataaagga tagggttaca atacttgaaa
    16141 cgcttgcaga ccagtctaga aatacaaaag atatagagct tcttactgaa tatgtaagaa
    16201 tagccttggc tagaagtatt tgtaataatt tagttgatga tgataagaca attgtagtgg
    16261 ctacattgtc tccagaaact gaagagttgg tatctaataa tttacaaagg tctgtaaatg
    16321 gaacttatcc agcagttgac ccagaaaata caaataaaat atttgagagt atacaagaag
    16381 taatgaacaa tgtatatttc aatcataata taccagtaat agtagtatca ccaaagataa
    16441 gagctccatt tagaaaatta gttgaaattg tatatcctaa tttgactgta ttatcattaa
    16501 atgaaatacc taacgatatt aaaatcaaag cagaaggtgt ggtgagcata tatgatgacg
    16561 aaaaagtata cagctaacac aattcaggat gctatgaatc tagccaaaat ggaattgggt
    16621 gacaatatta cattaataga taaaaaagaa gtaagaaagt ctggaataca aggtatcttt
    16681 tcaaagaaag atattgaact aactattgga tgggaaaaga gagaaaacgt agaacaaaaa
    16741 gatttaaaac gagagataga acaattaaag ttaattatta acaatatggg gtttgataat
    16801 aagaatgata atgatataga taaaatatgt aaaaatctac taagtttaga attaaatgaa
    16861 gaaattgtag aatctataag aattgatttg caagaaatga agttcaatgg aattgataca
    16921 agcaaaaatt tagtagaaat tttaaagaaa aagattaaaa tagaaaatca agcaataaat
    16981 ggtaaaattg ctctagtcgg accaccagga gtaggtaaaa cgactacaat agcaaagtta
    17041 gccgcaaaat tagtttttga agaaaataaa aaagtaggag taataactat agatacatac
    17101 aggattggtg ctgtagaaca gttgaagata tatactgata taatgaatat tccttttaaa
    17161 ggggtaataa gtcccgatga aatggaattg gcacttgatg agatgaaaga ttgtgatgtt
    17221 gtattaatag atacaacagg tagaggctat aaaaactcta tgcaaatact agaaatcaaa
    17281 aatcttatag ataaagctga aactgataat atacatttag ttgtgaattg tacaacaaga
    17341 gaaagtgata caaaagcaat tatagattca tatagaaatg tgaattttaa aagcttaata
    17401 attacaaagc tagatgaaac aataacatat ggctcaatat ttaacataat gaattatgca
    17461 caaaaaccaa tatcatatat tacaacagga caaaatgttc ctgatgatat tataaaacct
    17521 aatgaagata agattataag gttactatta ggagttgaaa gtatatgata gaccaagctc
    17581 aaattttgag aaaaatggca atggaaaaaa gaggacttga tgagtttata gtagagaatg
    17641 agaatgtacc taagataatt acaatagctt ctggtaaagg cggtgttgga aagagtaatc
    17701 tcgctacaaa tttgtctata tgcttgacca aacttaataa aaaagttctt atattagatg
    17761 cagacatagg aatgtctaat atagatataa taatgggggt aaatgtaaaa gggactataa
    17821 ttgatgtaat taatggcgaa aaaaatatag aagatataat ttcacagact aagtatggtg
    17881 taaatataat atctggaggt tctgccctta atcatataga agactttaca gaagctcaaa
    17941 gaaataagtt tatacatagc atagaacaga ttcacgatgt ggattttata ataattgata
    18001 caggagctgg tatgagcaaa agcctattgt catttatata ctgtagtact gaatttttct
    18061 taataacaac tccagaaccg acatcattga ctgacgcata tagtttatta aaagctataa
    18121 gtaattttgg cataaagaga agtgcaaata taataatcaa tagggtaatt gatttagaag
    18181 aagcaaaatc tacatacaaa agaataaaaa tggttgtgga caaatttcta aaccttaatc
    18241 tagaattgta tggatatata atagatgaca aaaaagtaag tttatgtgta aaaaaacaga
    18301 taccattcat tttagaatat cctacaagta ttgcatcaaa atgtattata gccatagcaa
    18361 agagaatggt aagtcagtca agagaagata ttgttgataa taaaagtttt ctaagaaaga
    18421 tgtttagtat atttaaaagc taaggaggcg tagttaatga atagagaaga attaataaaa
    18481 gagaatatgc ctcttgtaaa gagtatagca agaaaatttt ttataccagg gaaaggtttt
    18541 gaatatcaag acttagtaaa tagtggagtc attggattga ttgatgcaat aaataaattt
    18601 gatgctgaaa agggggcaaa gttttctagt tactcttata taaaaataaa atctgctata
    18661 ttagatgaaa ttagaaatca aagtccaatt tctaaacata atctaactaa agtaaataag
    18721 tataatagag tggtagaaaa gttgcaatct aaattattaa gagagccaac ttcatatgaa
    18781 attgcaaaag aattaaatgt ttcagaaaaa gaacttcatg atatcgaaag taatatagac
    18841 atgttaaata ttgtatcatt aaactatgta atctttgaag atacaaatga aactgtacaa
    18901 gatgtaataa gtgatagaga agaggaagct ccagaaaata taatagaaga agaagaaaag
    18961 ttagagattc tatctaaagc aattagtaat ttaaatgaaa gagaaaaatt aatactttca
    19021 ctgtactatt atgaagattt aaatctaaag gagataggga aagtgctagg tgtttcagaa
    19081 tcaagagttt cacaactcca cagaaaatca ataagaaact taagaaacaa gataaaagaa
    19141 cttaaatatt ctatatagat ggtgattgat aagatgatac aaattatatt aatagtagtg
    19201 tctattttaa taatagtaac tatagttata aatttacata aggaatcaaa aaataaagat
    19261 aacagatact ttgataaaat atttgagcaa gaatatgaag caaatgatag attaataatt
    19321 gcagaaaaaa gaagattctt tttagacaaa gtacttgctg aaataagaag taataatcat
    19381 ataaaagttt atgaaaatta taacctaata aaggctaatg aagaaagaaa ggaaaacata
    19441 tttatagaat caaaaaagga agtaagacaa gaaaatgagt tatctcttaa agtgaaacat
    19501 cttataagtt caggttatga tgatgtggaa atatgtaaga tgctagatat tggaaagggg
    19561 gaactgtcat taataaggag ttgttacaaa atataaggat attattatgt gataaaaaag
    19621 tattcttagg aataggtatt ggtatattaa tatcaacttt atgcataatg ctttttagta
    19681 ataatgatat gagtaaggca gagatagaaa aaaaggccag agcgttaggc atgaaatata
    19741 caagtgagat gaaagtttta gaatagatga tactgtttag acatttacaa aaaaggaggt
    19801 cataatgtga taagaggatt atatacagca gtttctgcta tgataactaa tcaacaaaag
    19861 caaaatgttg ttgtcaataa cctagctaat atggatacaa atggatataa aagtaaacaa
    19921 cttttgacta aaagttttga tgaattaaag ttggagggtt acaataacta cgcaaatgga
    19981 actaaacaaa agcagattat aggcgatata agtccaggta tttctatgga tgaaactatc
    20041 acaaatttta atcaaggacc tataaaaact actgataata aaatggatgt agctattcaa
    20101 ggtaagggat tttttctagt tagtgatgca gctggaaatc aattttacac tagaaatggg
    20161 aacttcagaa cagacaatca aggtgacctt ataacaagtg aagggtatta tgtacttggg
    20221 acgaatactg ctactggtgc tgtaggtcat ataaatgtag gaaatcaaaa atttgaagtg
    20281 gcaaaagata atacaatatc actagatgga aatgctatgt ataaattcaa tattgtagat
    20341 tttaatgact acaataactt aaaaaaagaa ggagataact tgtattctgg aggaggagct
    20401 atgaaagtaa ataatgcact tacaatacaa agtgcgatag agtcttctaa tgtagatatg
    20461 gtatctgaaa ttaataatat gatgaccata tcaagagaat atgaggcaaa tcaaaagata
    20521 attcaggcta tggattctaa attagcaaaa atagcaagtg agattggttc agtaagatag
    20581 gtgataggag gtgaattttt atgtacacaa tgttttatac tagtaaaaca gctttatcag
    20641 caaatcaaag taaactagat ataatatcta acaatatagc aaattctgat tcaacaggat
    20701 ataaaaaaat aaatatggaa tattctgatt tagtggatga agtactaaat agaccatcat
    20761 atcctacaaa tggcaatgat atatcgactg gaacaggagc taaggcaaca gctcccatga
    20821 gaatttatga acaaggtgca ttggccccta cagatagtaa gagtaatttg gcgatagatg
    20881 gagaaggatt ctttagagtg ataagaaaag atggtacata tgcatataca agaaatggtg
    20941 gatttaatat agatggttta ggtaagattg tagatgataa tggaaatata ttagatgttc
    21001 aatttgatgc aggatataac tataataata cgaatataga tagcacaaac ttaacagttt
    21061 caagaaatgg cgagttattc gtagataata aaaaaatagg aaaaatcaat ttatatcaac
    21121 ctataggaac tcaaaatttt atatctgaag gagatagttt attcgtcgca actgatgcag
    21181 cacagattaa acaagttgag aaagtagata tagttcaagg ctatagagag aggtcaaatg
    21241 tttctttaca agaagaattt gttgacttaa tagccactca aagagcattt caaatgaact
    21301 caaagggaat aaagacagca gatgaaatgt ggcaaatagc taataattta agagctaaat
    21361 aattatgtaa ggtgaggaat attcatgaat gatatgaaag agtacgattt tggcaaacct
    21421 cagagttatt caagagaaaa tttaacttgt ttaaattttt tattagctga attttgtaaa
    21481 aaatataaaa actatataat ttatgaactt aagtgtaatt ctagtatgat tgttgatagt
    21541 atagaccaag taaattatca gactttttta gatagagtaa cttcttcatg tgtagtagta
    21601 caaaatgcaa tggaacctgt tatgaaaaac ttttctttta gaatagatag aagtgtagct
    21661 gatatgtgga ttgatataac ttctggagga actggagtta taaaggataa tgatagagag
    21721 cttacagaac ttgataaaaa agtgctttta catcttatgg atgatttagt aagaaatatg
    21781 cacttatttg aaggttttga aaatatacaa gtattggata cacatacaca tattaattta
    21841 ccacaattat gttctccaac tgcaccagta tgcgttgtag atttaaaagt gctaaatgat
    21901 aatgagtata ttggaagtgt ttctctttgt tttccgtata atggtttaga ggctctatta
    21961 gaaagtatat ctgttataga attttttgat aatgatgtag gagatgattc agaggaattt
    22021 accaaaaaaa tctataatag tgtatctaat atagaattat cagttattgc agagatagga
    22081 aaagtatcaa ttaatgttga agacttactt aaactagaag ttggagatgt aatagtaaca
    22141 aataaaaaaa taaatgatta tatagatata tttgtagaaa attcaaaatc atatacagca
    22201 acaccaggat ttatatcaag caaaaaaggt gtaaaaataa atgatgctgt tggaaaagag
    22261 gtgtagttat ggacaatagt aatcttattt cagatgaaga agcaaagatg ttattaaatg
    22321 ataaagttga aagtgaagtt gatggggatg atgagttagc cttagatgtc tctgaagtta
    22381 atcgaaataa tatacaaagg atacttgacc ttaagttaga attatctgta gttataggga
    22441 gaactaagat gactctaaga gatattttaa atttacataa aggttctctt attgaactga
    22501 atactttagc agaccaagat gtggaaatac taatagataa taaggttttg gcttatggaa
    22561 aagtagtggt ggtagattta aattttggtg taaagataac aagtatagta agtgaagaag
    22621 acatggtaaa atctcttgtt tagagataac atcaattaat acttaaggca tgaggtggta
    22681 cttattttaa acttaaatga tataaaaaaa gatatactaa aactcaataa aaaagactca
    22741 tgttctctat ataatgaatc tattaataat ataaataata attgtatatc aactggatta
    22801 aaatttttaa aacaagcaag agatttaaac ccagatgatg ttgatatatt aaatctcaca
    22861 ggtcttgtaa acttactaaa atgtaatttt gatgatgctg tagaaagttt ctataagagt
    22921 tcactttgtg aaaaaactgc tctgtgtgaa aagtatataa acatgcttac atctgaggag
    22981 tttttaattt tctttgaaaa gtacaatcaa cttataaggt taataaataa aggaatgtat
    23041 aaagaagcta tacaagggtt taaacttata actgaccaat actgtgattt aatagaacct
    23101 tatgagttgc ttgccttact gtatgacaag gaagaagaat atataaaatt tgatgaatgt
    23161 ttagaagtat tgaagattat tgataaggaa aattatttat taaatcaaga aaataacaca
    23221 ttagatatgt ataatagtgc taatatacaa actaaaagta atgtaaaagt agatgattat
    23281 aagaatgagt ttttacataa aagtagtagt aaccataaaa ttaatgatta taataatgta
    23341 actaaagaaa aaagttcaag caaaaaccag aaaagttcag attctaataa gaaaaaaagt
    23401 attaaaaaaa ataagtacct atatatagcc ataatattag gaatattatt agtaggacaa
    23461 agtatatata gttcttataa gatgaataat ttaactgata aaatatctaa aaataatgaa
    23521 actaaaaatg aagagtcaat agattctaac ttgactaaga aagaaaataa tatagaagaa
    23581 aatgaagata tgagtaaggc ttctaatata aataagagta ataaaaacat taataaagag
    23641 gctgatgtaa aatctaaaaa aaatactgta gaaaatgaag atgataaaga ttctgactta
    23701 tttacagagg atgaattgat gtctaaggcg aatactttaa aatcagaaaa aaaaactaaa
    23761 tcttctatac aactatataa gaaagttgca gatataggtg aaaataaagg gaatacatct
    23821 gaagcaactt atcaagttgc tattctcagt gaaaagttaa aagactataa aactgctgaa
    23881 gaatattata aaatgtatgt agaaaattac tcagaaaaag atgcttattt tgatgaatct
    23941 tattataatt tagggatgat gtactataac aatggtgatt taaaaaattc taaattgaca
    24001 ttaaagaagt tagttaataa ggtaccaaat agtatgtata ataattcaaa agtaaaagaa
    24061 attttaaaag aagaatagtt aaattagata agaaaaataa attattttat tttaaaataa
    24121 tatgattata atatattgac ataccatttt aaaaaattta aaatattaat tataaaatga
    24181 ggagggataa aaaatggaat ttgaaaaagg gagtatttca attcatacag aaaatatttt
    24241 tcctataatt aaaaaatggt tatactctga taaagacata tttataagag agcttataag
    24301 taatggatgt gatgcagtaa gcaaacacaa aagacttgta tcactaggag aaatatcaga
    24361 aaataagtca tcagattata aaattacagt atctgtaaat aaaggagaag gtacacttaa
    24421 atttattgac aatggaattg gtatgactga agaagagata aaaaaataca taaatcaagt
    24481 tgctttttca ggtgctgaag atttctttaa taaatataag gataagatgg aagagtcaaa
    24541 tgacataata ggtcattttg gattaggatt ttattctgca tttatggtgt ctaaaaaggt
    24601 acaaatagat acactttcat atacagaagg agcgactcct gtaagatgga tatctgaagg
    24661 tggtacagaa tacgaaatat ctgaatcaga tgctagaaat gatagaggaa ctactataac
    24721 actatttatt gatgatgata gtaaagaatt tttagatgaa tttactgtaa gaggaataat
    24781 aaataaatac tgttctttct taccagtaga aatatattta gaagatgttg aaagattaga
    24841 aagagaagct aaagaagcag aagaaaaagc taaaaaacaa aaggaagatg gaaaagaaga
    24901 gattgtagat gcaaaagtaa ttgagccttt aaacgacact aatcctctat ggttaaaatc
    24961 tccaaaagac tgtactgatg aggagtataa agagttttat agaaaggtat tcaatgtatt
    25021 tgatgaacct ttattttgga tacatttaaa tgtagattat cctttcaatt taaagggtat
    25081 actttacttc cctaaattaa aaaatgagtt tgaactgact gaaggaaaag taaaattata
    25141 caacaatcaa gtgtttgtgg ctgacaatat aaaagaagtt ataccagaat ttttactttt
    25201 attaaaaggt gttatagact gcccagactt accattaaat gtatcaagaa gtttcttaca
    25261 aaatgacagg gatgtaagca aaatttctaa acatataatt aaaaaagtag ctgataagtt
    25321 aaaatctctt tgtaaaaatg aaagagaaga atacaataag ttttgggatg atatacaaat
    25381 atttattaaa tatggttgct taaaagatga aagtttctat gagaaggtaa aagaatgtat
    25441 attattcaaa actattgatg atgagtatat aacaatacaa gattatttag aaaaatgtaa
    25501 ggacaaacat gaaaataagg tattttatgt aagtgataag gaacagcaat ctcaatacat
    25561 taaactattt aaagaatacg acttaagtgc tgttgtttta aactcatcaa tagatactca
    25621 ttttatttca tttatggaat ataaagagaa tggagttaag tttaatagaa tagatgcaga
    25681 cctttctgat gtattaaaag ataaaaatga aaataaagat agtgaagaaa acaaagaaga
    25741 gatagctaag atagaaggtt tgtttaaaga agctgttgga gaaagagtga agaattattc
    25801 tgtagaagga ttgaaaaatg aagacactcc agctatggta ttagtatcag aacaatctat
    25861 tagaatggca gaaatgcagt caagatttgc tggaatggat ttaggaatga attttgagga
    25921 agaaaaaact cttgtaataa atgagaacag tccaataatc aaaaaattgg tttctcttaa
    25981 agatgatgag gaaaagaaag ataagattac gcttatttgt aaccagatag cagatttagc
    26041 tttactttct aataaagaat taaagccaga tgagttagat tcatttgtac aaagaagtaa
    26101 taagcttatg agtcttttga tagaattata agattgaatt tgaatacatt gaagcaaatt
    26161 tataaaaagt attcaatatc agtgatgaga aagaagtgtc tcaaaatgaa cttttcagtt
    26221 catgaggcgc tttttttatg tgaaattaaa tttattttaa tcatttcaac aatgaaaaat
    26281 atatttatct ctattttgag acagaccgtt ctttattttt ttatctttgg ttaattttta
    26341 aatatttaag taggttctaa taaaaaactc tatttagtta aatatagtta acttgatata
    26401 taataagata atacatataa ttaagtaaaa gattgttttt tagtttatgt ttaaatacat
    26461 aattacactt tgttaaaaac ataaaattat tactaaagta gaaataggag gttttagcat
    26521 gaattttaat tataatttgc cagtaaatat cttatttggg agaggcagaa ttaatgatgt
    26581 gggaaaagaa gtatctaaat atggcaaaaa agtattgata gtaacaggta aaaatagtac
    26641 aaagaaaact ggattacttg ataaaacaat agatttacta aaagactcta aaatagaata
    26701 tgaagttttt gatagagtag aacaaaatcc tcttataact actatatatt caggcgtaga
    26761 aattataaaa tcaactggat gtgattgtgt tttagggctt ggtggtggaa gcattatgga
    26821 tgctgccaag agtatagctt tttcttataa aaacccagga gatttaaatg attatatatt
    26881 tggaattaga caaggtgaag aagctttacc tataatctta gttccaacaa catcaggtac
    26941 aggtagtgaa ggaaattgtt ttgcagtact tacaaattca gaaactaaag ataaaaaatc
    27001 tataaagaag aatagtatgt atgcaaaagc atcaataatt gacccagaac ttatggttac
    27061 aatgccaaag catataattg catcagttgg atttgatgca cttgcccata atatggaagc
    27121 atatctttct aatggaagaa accctcttgc agatgtacaa gctatttatg ggattgaact
    27181 tatatcagaa aacctaataa aggtatataa tgatgttaat gatttagaag catgggaaaa
    27241 aataacactt gcaagtacta ttggaggaat gacgattggt acagcgggta cagccttgcc
    27301 acatggaatg gagcatcctg taagtggtct tagagatgta atgcatggaa aaggacttgc
    27361 agcgttgact cctactatta tagaaagttc atgggatatt gatattgaaa gatttggtaa
    27421 tatttcaaag gtattaggag gaacttgtgc agaagattgt tcagatgcaa ttagaaattt
    27481 ccttaaaaaa atagatttag atataaaact aagtgattta ggtattgaaa aatcagatat
    27541 agattggtta actgataatt gtatgaaaat atcagttgca aatattaaga gacatccaaa
    27601 gtattttaat aaggaacaaa taaaggaaat gtaccataag tctttataat ttgactttac
    27661 atatttagtt ttatataaaa attataaatt aataataaaa aattataagt aattaatagt
    27721 aaaaaaattg tgtatagaag ttatatatat aataaacttg tcttaagctt ttattttact
    27781 ttctaataaa taaaggagtt taaaaaaggg aagattaatt aagtaatatt ttataaaatt
    27841 cttcgattta aaaactttaa gtagaggtga aataaatgtt taatcccaaa agaataatat
    27901 tcgaaaaagg aactttagac actgatgtgg gcaaaaaaat atacaataaa tttagagata
    27961 ataaagatgt agagataatt aatatatcat caaataggat taaagaacac attccaggtg
    28021 aaaatctata tgaacaatat agaagtggga agcagactat ggtagttggg attagaaaaa
    28081 gtctgaagtt tcaaacatgt aaaccttctg ctaattatca actaccactt gtcagtggat
    28141 gtatgggaag atgtgagtat tgttatttga acacacaatt aggtgataaa ccatttataa
    28201 gggtgtttgt aaatgtagat gaaatacttg aacagacaaa aaaatatatt gaagatagaa
    28261 agccacaggt aaccatattt gaaggggcag ctacatcaga cccagttcca gtagagccat
    28321 atactaattc tttgaagaaa acaatagagt tttttggaag agaggaaaaa ggaagattta
    28381 gatttgttac taaatacagt gatgtggata gcttattgga tgctgaacat aataatcata
    28441 cagaaatacg atttagtatc aatactccta gaattataaa tgaacatgag cattatacag
    28501 cctctgcaaa taaaagaata gagtcagctg taaaacttgc tagggctggt tatcagattg
    28561 gtttcataat agcacctgtg tttttatatg aaggttggaa agaagagtat aaaaatctga
    28621 ttataagtat aaaagaagct ttaccaaagg attttgataa aaaaattata tttgaagtta
    28681 tatctcatag atatacaact aaagcaaaaa atagaatact agagatttat ccagatacac
    28741 tattgccaat ggaaaatgag gatagaaagt ttaaatacgg tcaatttgga tatggtaagt
    28801 acgtgtatga taaagaacaa ttagatgata tgaaagagtt ctttcttaag gaaataaatg
    28861 gagtatttaa aaatagttta ataaaatata taatatagca aatcttacct aaagatttgc
    28921 tatattttat ttttatatat tttatgttta taataaagca atgagattta gcactatcat
    28981 ataagaaaat taaaaagggc actatctatc aagaacattt tttctagttt tgatactata
    29041 aagggtaaga gtaacaaaaa tatcaggagg agttatagta tgcatgcatg ggagtctatt
    29101 caaattacat tggatttaat agaagcaaat ttatcagaag aaatatcaat taatgaatta
    29161 gcaaataaag caaatttatc accattttac tatcaaagac tatttaaaag attggtaaat
    29221 aagactgtaa tggagtatat aaagcttcgt cgtcttgcaa gagcttctga atacttagca
    29281 gaacataaga atcgtatact tgatgtagct ttaaattttg gatttgctag tcatgagaca
    29341 tttacacgtt cctttaaaaa agcatatgga ttaactccag aaaaatatag agcaaatcca
    29401 gtaaaactca atcactttat taagccagaa ttaattttaa attatgctat ggttgatgaa
    29461 ggtgttccac tgattgcaga tgatatagtg attgaagtca atcgtaagac attgagtaat
    29521 ccaaggacat ttgttggaat agaaatagaa gtacctattt gccagttggt gggtggtgaa
    29581 acaactggaa ttgcaatagt agaggaatta tggatgaaac ttggaagtca aagacataat
    29641 ataccaaatc aaattccagg tggaaatgaa tttgctgctc tttatatggg aaatgcaaaa
    29701 gaaggaaatt gtatgtatat ggcaggtgta gaggtagagt caggtacatc agtagaagga
    29761 tataatactt ttgagttgcc agctaaagaa tatttagtat gtggatttga ggcagaaaac
    29821 tttaatgagt tagttaattc agctgttttt aaagcagata aatttatgga aagatggatg
    29881 aagaaacata atttaacaac tactgatttt gcaattgaga tgtattaccc cactacacct
    29941 gaagcagcat atctggaaca ttggattgtt ccggttccta ttgaacaata gaaaggatga
    30001 tgaatatgaa ttggcaagat gcgttttttc aagatactaa acctacattt gagcaaatta
    30061 atgaatttat taataatcca ttatgggaca agttaaataa aactttggtt tctacttata
    30121 atgtagaacc taagcttgaa tacagtaaat gttcaatgca aagaggttgg aatgtaaaat
    30181 ataaaaaaag aggtaaatca ctatgtactt tatatccaca agaaggttat tttaaagctt
    30241 tggttattgt aagtgaaagc aatagagtag aggttgattt atttattaat acttgttgtg
    30301 attatatcaa aaaaatatat aatgaggtaa acttttttaa tggaagtaaa tggctaatga
    30361 ttcaagtaga tagtttattg gtattaaacg atatgcttga actaattaaa tttagagctt
    30421 aacatataga acaggatgaa tatataagtc atcctgtttt tatattcaat cagataaatt
    30481 aatatatagt ctataatatg aataatttag taaatttact ttatctttct acattctttg
    30541 caactaagtc cacatgtgtt tttttcttgc atattagtag caccccaatt agcaaattgt
    30601 tgtaaaatag gcacaatact tttaccttta cttgttaatg tatactcaac ttttggagga
    30661 atttcattat attgtttacg atgaacaata ccagtagact ctaaatcttt taatgattga
    30721 gctagcatag tatttgtaat gccatccaat tttcttttta tgctactata tctaattgga
    30781 ttttcaactg ctaacataca tataataggt aatttccact taccaccgac aatgtctaat
    30841 gcataacgga ttggacaggt taattcttca acgtttttag ttatattttt ttcagtattt
    30901 atactcattt taattactcc ttagttatag tagtatcttt tttatacttt atcagaaaaa
    30961 actgcatact tgttttttct gtattttata ttaaaattat atcaaaggat aatccttaat
    31021 acaagtattt aaaaggggtg taagaattat gaaagcatta gaatttttga acaaggcagg
    31081 agtttattat ttagcaacag ttgatgagaa caatcaagca catgtgcgtc ctttaggatt
    31141 tgttatggaa tacaatggta aacttacatt ttgcacatct aatcaaaagg atatgtttaa
    31201 gcaacttatt tttaatccaa aggttgaact ttgttgtata gaccaaaacc ttaatactct
    31261 acgtatttta ggtgaagcag tatttgttac atcaaaagaa acacaagcta aggcacttga
    31321 aataatgcca agtttaggtc agatgtactc tgtgggagat ggtaagtttg aaatattctc
    31381 tattgataag gcaaaagtta gttgttgttc aatgagtggt aaaaagattg atgtagagct
    31441 ttaattataa ttgaatcgac atccatagtg aaaattaaga aaacgttatt actgaaatat
    31501 tgatagattt aatttaagtg caataaaaag atttttaaat aatgaatatg taaaaatttt
    31561 tagttattag atgtgtattt caataatgat atactatttt gaataattac atactagaaa
    31621 taagataatt aaaatttact ttataatggg atagatttct tttaaaaaat attttaacta
    31681 taattgattt ttatttaatc tgaaaaaaat attatgacta taaaaaattg ctacttatta
    31741 tttggatatt tttgtatata aataataagt agcatgactt tatacattaa taatgataga
    31801 atataaatta atagataaga tataatttta aaaggagtga tataatatga gtaaatatag
    31861 ttctttatgg gaatatgtta agaaaaataa tagtcaatcg tttaaattaa cttttgaaga
    31921 aataaaagat attgctggaa tagagataga ccattcgttt ttgaaatata aaaaagaatt
    31981 aaatgaatat ggatatcaag taggaaaaat ctctttaaaa gagaagactg ttattttcaa
    32041 taa
//
LOCUS       DAGQWR010000032        31548 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.032, whole genome shotgun sequence.
ACCESSION   DAGQWR010000032 DAGQWR010000000
VERSION     DAGQWR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.032"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..607
                     /locus_tag="MIW95_001269"
     CDS             <1..607
                     /locus_tag="MIW95_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBY3150611.1"
                     /translation="LYYCPKLTKNILPYSYTVEKIICTLLVIIMALEQLLLISSGNYS
                     TLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSLVCSVGELIFSKNLGYEFPSLI
                     YFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAICFIYFSFIFLLNTFTNSQYYY
                     GFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKKKKSK"
     gene            complement(698..2149)
                     /locus_tag="MIW95_001270"
     CDS             complement(698..2149)
                     /locus_tag="MIW95_001270"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBY3150612.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAIETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(2165..2605)
                     /locus_tag="MIW95_001271"
     CDS             complement(2165..2605)
                     /locus_tag="MIW95_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY3150613.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            complement(2853..3281)
                     /gene="ytfJ"
                     /locus_tag="MIW95_001272"
     CDS             complement(2853..3281)
                     /gene="ytfJ"
                     /locus_tag="MIW95_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBY3150614.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            complement(3235..3864)
                     /locus_tag="MIW95_001273"
     CDS             complement(3235..3864)
                     /locus_tag="MIW95_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBY3150615.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADINNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTKSYGNNS"
     gene            complement(4026..4565)
                     /gene="scpB"
                     /locus_tag="MIW95_001274"
     CDS             complement(4026..4565)
                     /gene="scpB"
                     /locus_tag="MIW95_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBY3150616.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            complement(4567..5286)
                     /locus_tag="MIW95_001275"
     CDS             complement(4567..5286)
                     /locus_tag="MIW95_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBY3150617.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(5316..5912)
                     /locus_tag="MIW95_001276"
     CDS             complement(5316..5912)
                     /locus_tag="MIW95_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBY3150618.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLIYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
     gene            6210..7046
                     /locus_tag="MIW95_001277"
     CDS             6210..7046
                     /locus_tag="MIW95_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY3150619.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            7067..7489
                     /locus_tag="MIW95_001278"
     CDS             7067..7489
                     /locus_tag="MIW95_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBY3150620.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(7700..8863)
                     /locus_tag="MIW95_001279"
     CDS             complement(7700..8863)
                     /locus_tag="MIW95_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBY3150621.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(8917..9117)
                     /locus_tag="MIW95_001280"
     CDS             complement(8917..9117)
                     /locus_tag="MIW95_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBY3150622.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            complement(9323..10999)
                     /locus_tag="MIW95_001281"
     CDS             complement(9323..10999)
                     /locus_tag="MIW95_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBY3150623.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            11216..11953
                     /locus_tag="MIW95_001282"
     CDS             11216..11953
                     /locus_tag="MIW95_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBY3150624.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            complement(12070..12528)
                     /locus_tag="MIW95_001283"
     CDS             complement(12070..12528)
                     /locus_tag="MIW95_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBY3150625.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(12810..13097)
                     /locus_tag="MIW95_001284"
     CDS             complement(12810..13097)
                     /locus_tag="MIW95_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBY3150626.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            complement(13175..13600)
                     /gene="ruvX"
                     /locus_tag="MIW95_001285"
     CDS             complement(13175..13600)
                     /gene="ruvX"
                     /locus_tag="MIW95_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBY3150627.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            complement(13806..15977)
                     /locus_tag="MIW95_001286"
     CDS             complement(13806..15977)
                     /locus_tag="MIW95_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150628.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYGTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNVGYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIININMIIFEKNN"
     gene            complement(15987..16244)
                     /locus_tag="MIW95_001287"
     CDS             complement(15987..16244)
                     /locus_tag="MIW95_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBY3150629.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            complement(16269..18908)
                     /gene="alaS"
                     /locus_tag="MIW95_001288"
     CDS             complement(16269..18908)
                     /gene="alaS"
                     /locus_tag="MIW95_001288"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBY3150630.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     misc_binding    complement(18976..19214)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(19357..20436)
                     /gene="mnmA"
                     /locus_tag="MIW95_001289"
     CDS             complement(19357..20436)
                     /gene="mnmA"
                     /locus_tag="MIW95_001289"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBY3150631.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     gene            complement(20725..21165)
                     /gene="nifU"
                     /locus_tag="MIW95_001290"
     CDS             complement(20725..21165)
                     /gene="nifU"
                     /locus_tag="MIW95_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBY3150632.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            complement(21219..22412)
                     /gene="nifS"
                     /locus_tag="MIW95_001291"
     CDS             complement(21219..22412)
                     /gene="nifS"
                     /locus_tag="MIW95_001291"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBY3150633.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGRVDLRQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            complement(22430..22876)
                     /locus_tag="MIW95_001292"
     CDS             complement(22430..22876)
                     /locus_tag="MIW95_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBY3150634.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            complement(22985..23755)
                     /locus_tag="MIW95_001293"
     CDS             complement(22985..23755)
                     /locus_tag="MIW95_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3150635.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDTLAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            complement(23786..25051)
                     /locus_tag="MIW95_001294"
     CDS             complement(23786..25051)
                     /locus_tag="MIW95_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBY3150636.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            complement(25158..25943)
                     /gene="codY"
                     /locus_tag="MIW95_001295"
     CDS             complement(25158..25943)
                     /gene="codY"
                     /locus_tag="MIW95_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBY3150637.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            complement(26108..28195)
                     /gene="topA"
                     /locus_tag="MIW95_001296"
     CDS             complement(26108..28195)
                     /gene="topA"
                     /locus_tag="MIW95_001296"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBY3150638.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            complement(28228..29328)
                     /gene="dprA"
                     /locus_tag="MIW95_001297"
     CDS             complement(28228..29328)
                     /gene="dprA"
                     /locus_tag="MIW95_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBY3150639.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGYAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            complement(29347..30948)
                     /locus_tag="MIW95_001298"
     CDS             complement(29347..30948)
                     /locus_tag="MIW95_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBY3150640.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLLVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVSIMSFLDDKSRKENGELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISIEEMIEVSKV
                     YSILGMINESKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(30979..31323)
                     /locus_tag="MIW95_001299"
     CDS             complement(30979..31323)
                     /locus_tag="MIW95_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBY3150641.1"
                     /translation="MNNKEKGDFGEEIAVNYLLSKGAKILEKNYRLRIGEVDIIANIG
                     DEVVFVEVKSRSNIKYGYPCESVDFKKRKKIIGVAKYYIIKNNLNNTPIRFDIIEVYL
                     LEKRINHIMNAF"
BASE COUNT         9814 a         5672 c         3248 g        12814 t
ORIGIN      
        1 tttatactat tgtccaaaac taaccaaaaa tattttacct tatagctata cagttgagaa
       61 gataatttgt acattacttg taataattat ggctctagag caattattac ttatttcttc
      121 aggtaattat agtactctaa actctttacc aattggaatc aattatatct gtatatatct
      181 gtgtatagcc atactaattt ttaagcagta tcatcttttt aatatatttt tttcttggag
      241 tttagtttgt tcagttggag aactcatttt ttctaaaaac ttgggctatg aatttcctag
      301 cttaatatat tttatattta tcttctctaa gtgcttgata atttatgcag atatatatat
      361 ggttgatgtt agaaaattta gagttaacag atatgcactt agagataact tagctatatg
      421 ttttatttat ttttcattca tatttttact aaacacattt accaactcac aatactatta
      481 tggtttttta agtcatagca caactgctat ttttacgttt atttttgtta ctagtattat
      541 gtacatacct gctcttttat ttaatagaga tacttttata cttgagaaaa agaaaaagag
      601 taaataattt acttaaataa aaaataaagc cacatagata atcttaaatt acaaaaatat
      661 ccatgtggct ttataaatta tattttataa ttgtttttta gatttctgcc aaatagtata
      721 atgttctaac aggttctcct gtagccccct ttgctttgta tccaattgat tttgatgatg
      781 tacttgtccc cgctatatcc atatgtatcc aaggagtatt cccaacaaat tcaccaataa
      841 aagctcctgc tgttatacat ccagcatttt taccaccagt gtttttaagg tctgcttctt
      901 ctccttttat catatcttta aattcatcat aaattggcat cttccatacc ctttctcctg
      961 ataaagtggc agctttttct aattcacaat aaaaatcatc attatttgtt actataggag
     1021 ttgcaacatt tcctaacaat gttaaagcag caccagttaa tgtagctaca tcaactacct
     1081 ttgttacttt ttcattgttt ataatgtagt atattgcatc agctaatgta agtctacctt
     1141 cggcatccgt atttaaaact tcaatagttt tctttgccat agaactaatt atatctccag
     1201 gtttgtatga acctcctgat aaagcattct cacatgcagc cactactgct ataacatttt
     1261 tctttaattt actttgagat atagcacaca tagcacctat aacactcgca gcaccagcca
     1321 tatctgtctt catatctagc atagaagcat ttgattttaa agaatatcca ccagtatcat
     1381 aagtcaatcc tttacctact agacctaata tatctgtact ttctttatct ccaaagtatc
     1441 tcattactat gaatcttggt ttattgattg aacctcttga aacggcgaaa aaagcatcca
     1501 ttcccaatgc ctttattttt tcttcttcat aaacatcaac attaaacccg aactctgcac
     1561 caagtttaac tgtttctatt gctaatgttt ttggatatat tacgtttgat ggttcattaa
     1621 ctagatttct agctattata gttgaattag ctaatatttt agccttttct atttgacact
     1681 caacttcttt ttcagtaaat atagttatag tatcaacttc ttttctttca aaatccttac
     1741 tttttgtctt atacttatca aacttataat ttccaagtaa tatcccttct acaaaagaac
     1801 ctatttcatc actatttaaa ttaagattaa gtacattaac atcactactc ttatatgctt
     1861 catttttcaa tatatttttt aattttttac acaaattacc acctatgatt ctaatttttt
     1921 ctctattcaa taactctctc ttaccaaaac caacaagaca aacatttacc aagttgttat
     1981 tttcaaccaa tgttacagta tctatactat tttctttaga attaaaattt ctgcttatca
     2041 tatttatttt atcaaatgtc gtagaaacat cttctccctc aaatagagct actactgtta
     2101 tagcattttc taaacttttt aatatctctt tagaatactt tatattcata ataaattctc
     2161 ctttttaaaa taattgttct attatatttt tagactcttc caaagaagta aataatttat
     2221 ctttattatc tatatctaag caatccattt tatcacttat tatagataca aactttagta
     2281 tttcattatc caatatcttt tctccatttt tagttattcc ataaaaagta tttcgtctat
     2341 ctctactatc ttcttcccta tatacatact cttgctcaac caatttatta agtagtatac
     2401 acagactaga agtagataca tgtaaatctc tacttagatt tttaagctct attttatcat
     2461 gttttcttat ttttactaag gcaaagaact gtctttctgt aagaacctta tcttttttat
     2521 atctttcgtt attaataatc tttattaaat tatgaaaatt attcatactt tctaacaaca
     2581 aaatagataa ttctttatcg ctcataacat tcacctcaaa actaatgatt tataactccc
     2641 tcttatatct caaatgcgtt tatatatcta taatataata cttcttttct ttttataatt
     2701 atactatata tcaactttat tatctattta aattgtttta aactaaaatc ttattagata
     2761 taaatgttta aagtacacaa caaaatagtg ggatttatac taaccccact actttactta
     2821 aaactatata catatttaaa aatattttat tcttacatat tgtttttatc ttgattctta
     2881 ttactttgtg atatattttg aattttctcc aatactctag gagttataga taatatatta
     2941 tccactagat tgatattact gtctaaactc attattctag tctctccact ttcaactact
     3001 acaaatgcta ctggttgtac agatatagcc cctgcgctac ctccagcaaa attcgtatct
     3061 gatttttcat tttttaattc aatttcccta tctttatcat catatccttt tgagtattct
     3121 cctcctccta taccaaagcc aattgtaacc tttgatatag gtacaaccac tgtagtatct
     3181 gtttttatag ggtctcctat aatagtattt gcatctatag aacctttaat tgtttcaaga
     3241 gttgtttcca taagactttg tatcgaacct gttgctctca ttactatcac cttccttatt
     3301 tcctttattt ttattcataa ttttattaat cattattata atattaaata aagcttttat
     3361 tcttggcctt atatgtatcc ttatatttcc taaaacataa tcttttgtaa aatctggatt
     3421 tacatttaaa tacatttttt ctgcatcaat catattaaat aaatttccat acaaagtatt
     3481 tatcaataca tatacagaag atgttaagcc aatatttcca gttccaaaat taatatttga
     3541 ataaagttca tgtattttta tcttttttaa taatctataa atgctcaaaa ggtcatcagc
     3601 taataatata cttgaatcta tattattctt catcccctct tttttatcat tatttttact
     3661 attttcagct ggataaagtt gtctatttat atttattaaa ttaaacatgt attttatatt
     3721 aagctttaaa caaatatctt tgttgtttat atcagctatt attaagatgt ttatatgaga
     3781 tattaaaatc aacatagtta cacttataat aaaaattaca gctattatta aatacttaaa
     3841 attaaataat tcattaagct ccatgtttat attatcatca aaaaattact atttatactt
     3901 aattatccat atttatttta agagaattat aattttatat ttatacaaaa aaacacaaca
     3961 acttagagca ttttaaatct tttaaaattt aaaattacat actaaattat tgtgatttta
     4021 tatttttatt catttgttgc cacttcttga taattttcta taggcggcaa ttcttcaaga
     4081 ctttctatat tcaacaactt taaaaattca tctgttgttt tataaattat tggtttccct
     4141 atcttattca atctaccagc ttctcttata agttcatttt caaacaaagt ttgtaatacc
     4201 ttatcacatt ttactcctct tatatcctct atttctacct ttgttattgg ttgtttgtag
     4261 gcaattatag taagagtttc taatgtagct tggctcaaac ttttcttttt ctttggttca
     4321 ataatcttct ttatatattc tgcatagtct ttatttgtac acatttggta cttattctct
     4381 agcttgatta tttgaatacc tctattttgt tctctgtact cctcaataag taaatttaac
     4441 ataatctcta tttcttttga tgaaagttct ttatttatta tatagtttaa ttcttttata
     4501 cttattggtt ccccatatgc aaacataaca gattctataa tatactttat atcttctctt
     4561 ttcattttaa cactccgaac tttttcttat tagaatatca tcaaaaaata tgtcttgtac
     4621 tactactatt tctttttctt ttataagctc taatatagat aaaaaagtag ctattatttc
     4681 atcattttca tatgatttta taatatttgt aaaagtaact tcaatcttat cttttataat
     4741 atctcttata tacaatatct tttcttccac agatattatc ttcttcctta ctattttgtc
     4801 tagtttctca tcattagcat tttctatctg tgatgtttta actttaaata tgtatggaag
     4861 tatatttttt attgcttcaa ttgatatatc ttctaaatcc acattgtcat caataattat
     4921 ctcttcttta tttctataaa atctctcatt aacataagtt atattttctt ttatatcttg
     4981 tgatgctacc ttaaactttc tatattcttc taacttttcc ataagttcta ttcttgggtc
     5041 ttcttcttca tctttttgct tataaagcag atatttagac tttatctcta aaagttttga
     5101 tgccatagtt ataaattcac tagttatctc taaatccatt ttatctagca ttttcaaata
     5161 atttagatac tgcttagtta tatctattat agatatatcc tttatatcta ttttatgctt
     5221 tgttattaaa tcataaagta aatctaatgg tccctcatag acttgtaatt gtatattata
     5281 cttcatatca atccctcttt aatctgttta ttatattaaa caaataaata tactattttt
     5341 agcaacactg tatatattgg tgatagtatt atttgatata taccagtaaa taaagcaatt
     5401 aataaaatta tataactcat actttcaaat ttatacaaat attcattcca tttatatggt
     5461 acaaatgtag atataactcc ccatccatca agtggaggta tcggaagtaa gttaaaagct
     5521 gcaaatccca tactatatat aagcacatat ccagctattg tatttatagc atacatatgc
     5581 acatacttat tgataattac acacagtata gcagtcaaaa cattacaaaa aacgcctgca
     5641 agtgaaacta tcaggtttcc tatcttatag tttttaaaat tatttggatt tactggtact
     5701 ggctttgccc accctatctt aaggataaac atagccaaaa gacctattgg atcaacatgt
     5761 cttacaggat ttaatgtcat tctaccataa gctttagctg tatcatctcc caataagtga
     5821 gctgaataag catggccaaa ctcatgtatt gatattgcca tagctatacc aactaaactt
     5881 gctactattt ctcctatatt catacttaac attaatgtac atctcctaaa ttaaattttt
     5941 aatattatta taacaattaa atagcttata atcaaaatta ttttacatta taataatttt
     6001 aacttctagg cataaaatta aaatttgctt tttattttat aacttttatt attatatcat
     6061 attcattgtg aaaatataat aattacacct actgtaagtg taatattata ttgacatgaa
     6121 ttgacattat tgtctcttag tcaatattat acaagaagta taaaaaaata tgtgttacac
     6181 tttgtataac atgaaaggag gttacctaag tgagacaaga agaacgcaca gtatgctttg
     6241 atagggaatt aaaaattgaa gcatatagat ttaaaggcat aatgcaaaaa ttcccaaacc
     6301 attttcatga acattatgta attggtttta tagaaaaagg tcaaagatat ttatcttgta
     6361 aaaataaaga gtataccaca agtactggag atttgctact ttttaaccct tttgacagtc
     6421 atacttgtga acaaatagat gataaagtac ttgattacag atgtatcaat attaagccag
     6481 aaataatgaa aaaaaccgtc tttgaaatta caggtaaaaa ttatttacca aaatttaatc
     6541 aacctgttat atttcgcagt gagctagtac ctctactaca agaattacac tatataatca
     6601 tggaagaaga attggatttt aaaaaggaag agcttttttt cttcctaatt gagcaattaa
     6661 ttgaagaaca tacagagcca aatttgcaat ctaacttgga aaatacaaat atagaaattc
     6721 aagcagtatg tgattatcta gaaaataatt atgctgaaca tatagtactt gacgaattaa
     6781 gcactatagc aggtatgaat aagtactctc tacttcgtaa ttttacgaaa ttaaaaggta
     6841 ttacaccata tagataccta gaaaatatac gggtaaacaa agctaaaaag cttttagaaa
     6901 aaggcgtaga acctatagat gctgcgatac aaacaggatt tgtagaccaa agtcacttta
     6961 caaacttttt taagaatttt attggtctta caccaaagca gtatcaaaat atatttataa
     7021 atgatgattc aaacaacttt aaataaggta aaaaggagaa ataaaaatgg aaaataaaaa
     7081 ttcaaaatgt gtcatggtaa tagacgaaaa tttaccaatg ggaattatat ccaatacagc
     7141 agctataatg ggaattacac taggaaaaca tgccccagaa actgttgggc cagatgtaat
     7201 tgacaaaaca ggtaatagcc acttaggaat tattgacatt cctgtaccta ttttaaaagg
     7261 aaacaaagaa ataataaaag atttacgtaa aaaattatat actttggaat ttaacgattt
     7321 aactgttgta gatttttcag atgtagcaca aagttgcaat ctttatgaag aatttacaca
     7381 aaagattgct agtgttccag aagatgagtt acagtatttt gggatagcaa tttatgggaa
     7441 taaaaagaaa gtgaataaac ttactggtag tatgccttta cttagataat tgttaaacaa
     7501 ccctatatat gatattaaat caaatgatat tttaagatac ataaactttt ctatatttct
     7561 ataaaaatca taagattata aaatacttct atatggtaaa taaaaatacc cctatggcaa
     7621 ataactacat aagggtattt tttatgtgta ttataatcat atctttgtgt attataatta
     7681 tatttcatct gataattatt tatatcatat tatcaattat tctttgaagc atttgtagat
     7741 agcttgcctt atttatatcc ttagtattta tcaattccac ttcgcctatt acttttttat
     7801 ctttacatat ctttattttt cctaaaacag ttcccttttt aattggcatc ttaggatttt
     7861 ttactatttc aattttcttc tcaaattctt tagagcttcc ttttttaata agagcattta
     7921 aatcttcttt agcaaccaat ttaactttgt tttcatcagc cttatccaat gtaagtgttg
     7981 ctatattatc acctttacta cataacttaa cactttcata attagcaaat ccatagttta
     8041 aaaggctaga ggcatcatta aatctttctg gagaagtctc tgctcctaaa gttgcagcaa
     8101 ctagatgagt attacctctc tttgcagatg ctgataaaca gtattttgct tcttgagtaa
     8161 aaccagtttt aacaccagtt gctccttgat aatgctttat aagtttatta gtatttgcaa
     8221 gacctactgt aacttgtttc ttccctacta caaccttatc catccatgta gttaagtatt
     8281 tacttatcat ttcgtgtttt aatagttctc tagacattaa agctatatca tgagcagatg
     8341 tatagtggtt tgcaactgga agtccatttg tatttgcaaa atgagtatca ttcatattta
     8401 actcttttgc tttagcattc attctatcaa caaatccttc aacacttcca gctaagtgtt
     8461 cagccatagc aacacaagca tcatttgcag aagcaacagc tatacctttt aataatgtat
     8521 caaccttttg agtttctcct ggctctagga atatttgact accacccata ctagatgcag
     8581 tttcacttac ttgaacttca tcatcaagct taatcttgcc tgaatccaaa gcttctacta
     8641 taagtagcat agtcattacc ttagttacac tagcaggagg aagcttttta tgagcatcct
     8701 tttcatataa aatttgtcca ctaccaacat ccatcaaaat tgctgatttt gaagatatat
     8761 ttagttggtt ggcatcagca ttttccttat tatttgcaaa agaaaagctt aaaggagtaa
     8821 gcgcaattaa tatagccaaa gcagtactag ctaatttttt catcgatgtc acctcttaaa
     8881 gtttatttaa acttattttc tcacatttaa ctttttttat tctaaatgcc tagatatacc
     8941 ttgacctaaa ttttttttct tttcaagctt tttaaagtaa ccttctaaaa cacctttatt
     9001 agacgcattt tctattattt caaaagcatc ttcataattc attccatact ctcctgctat
     9061 ttccattttt gtttgtttta aagcttttat agcattttgt cttgaaacat catccataag
     9121 aatcctccta tcagtaaaat ttatatataa gtagttttta ctaataaaag attgttataa
     9181 ttggtatttt atgtaatata tatttttttt aaatgagtat taaaaagacc tcttgtaatt
     9241 cttaagataa tatcaattgg atttttgata ttactttaaa aattacaaga ggtctaattt
     9301 tcaaatttat agttttttta tattatactt ccattatgat aggaagtatc attggatttc
     9361 gtttagtttt ttgatataaa tattctttta ggttttcttt aatattattc tttaaataag
     9421 cccattcttt aatatttttc tcttcacatt catttagaac attcttaatt atatcctttg
     9481 ctccatccat taaatcttct gactctctta catatacgaa tcctctggaa attatatctg
     9541 gacctgctaa gaccttaccc tcatccttgg aaatagtaac tacaactatc ataagaccat
     9601 cttctgataa atgctttcta tcccttaaaa ctatattacc tacatcgcct actcctagac
     9661 catcaactaa tatgtttcct gtttgtatac ggtttgctac cttagcagag tttttatcta
     9721 attctaaaac atctcctgtt tgcatgacaa aaatattttc tttatccata ccaagctgtt
     9781 cagctatttc agcatgtctt tttaacattc tatattcacc atgagctggc ataaaaaact
     9841 ttggtctaac taatgcatgt ataagtttta attcttcttg acaggcatgt ccagaaacgt
     9901 gtatatcagc tatatcactg tatactactt ctgctccttt ttcaaataag aaattgatta
     9961 ctcttgatat taatttttca tttcctggta ttggatgagc agatattata actaaatcgc
    10021 cactctttat ttctactttt ttgtgctctg aaaaagccat tctagcaaga gctgacatag
    10081 gttcaccttg tgaaccagtt gttattatca ctaattcact atcttcatac ttacatatat
    10141 catttaaatc tataagcatg tcatcagaaa tatctaggta tcctaattct tttgcaactc
    10201 cgactacatt gaccattgag cgacctgata tagcaacttt tctattaaac ttttcagcag
    10261 tatttattat ttgttgtaat ctgtgtatgt tggatgcaaa ggtagcaact ataattcttc
    10321 tgccatttcc ttttgcaaat aattcgtcaa gacctacccc aacagtcttt tcagacatag
    10381 taaatcctgg tttttctgca ttagtactat ctgccagcat taaaagaact ccttttttgc
    10441 tcaactcaca gattctatgc atatccataa catctccatc aataggagtc aagtcaatct
    10501 taaaatctcc tgtgtggtat attatacctt ggtctgtgtg tattgcaata gagtaagcat
    10561 ctggtatact atggttattt ttcaaaaact ctacttccat attatcaagc tttataactt
    10621 gtctaggacc tataacattt aacttaacat tattcatttt atgttcttta agttttactt
    10681 gtataagacc tatactaagt cttgccccat atacaggtac atttattttc tttaatatat
    10741 atggaagagc tcctatatgg tcttcatgtc catgtgtaat aaatataccc tttattttat
    10801 ccctattttt tactaaataa gttatatctg gtataactat atctactcct aacatttcat
    10861 cctctggaaa actaagtcca gcatctataa caattatttc atctttatac tcaacaacag
    10921 tcatattttt tccaacttcg ttaagtccac ccaaggccat tacctttatc ttgttggtgt
    10981 tctttttgaa caattgcatc atctccttct gtctatttaa ttttttaaca cgttctacaa
    11041 tcataaataa tatcaaatca cgcactcgct accagttatt cttattgtac cataattcgt
    11101 atcatatttt aagctatatt taaaatacat ttaacgtatt attttgacaa acctatttta
    11161 ttttgatatc ctctaaatat tatatgattt aatatcggat aatttaggag gatttatgcg
    11221 tggtaaaact cattgtgcaa taggaatctt aacagctatc caaacatctt taatatttaa
    11281 aattcctata tctttagttg atattttagt ttcagctact tttgctgtac tacccgattt
    11341 ggataaatct aattctatgg tctcaaattt cattctaaaa aacaatgtat ccaaatacat
    11401 atatagaatt tttatatatg ctgttaatat tattattttc tttatttcaa taaatataaa
    11461 tgataatttt tatttaagcg caataattac atttgttgca attataatta ttgaagcaaa
    11521 actaacccac acatttctaa gaaaagtttt tctatcatta atatttatac tattagctat
    11581 atgcttatac atcatagaag ttgaaatata ttttacaatt ttttgtctaa tgttgtcaat
    11641 ttttccctgg ttaaagcaca gaagtttttc tcatagcata tttgcaacta taatagtata
    11701 ttttttacta aaacagatag aattaatcac taatattaat aatttatctt tttatggtac
    11761 gattggttat gcaagtcata tgtttttagg agatttattt acaaagcaag gaataccaat
    11821 tttttatccg ctaagcgaaa agaaaatttc tttaggtttt ttaactgttg gaggaccatt
    11881 tagtaatttc atagaaaaat cattcatttt tgtacttata ggattaatta tattttcaat
    11941 attaaaacta taagcatctt gaaaactgta tatttgcatt ttgtagatgg tttataataa
    12001 aaaatggaag aatagcatag aatataaaag ttctatacta ttcttccatc gacattaagc
    12061 aatagaagtt tattcttctg aatcgcttac tccattacac tcgtcacata atccataaaa
    12121 tttaacatca tgatcaagta ttttaaattt gtacaatgat tgtatctctt tttctatatt
    12181 gtctaaaagg tcttctttag cttccattat ctttccacaa tttttacaaa taagatgatg
    12241 atgattatga cagtcttctt tatgtagact aatctcatat cttatacatc catcgtctaa
    12301 gtttagtttt gatattaatc ctatttcatc caatagctgc attgttctat atacagtagc
    12361 taagcctatt tcaggacagt caaccctaac taaatcatag atttcctcac tacttagatg
    12421 tgaatggtca tgtttaagca atatttcaac tattgctctt ctttgtggcg taatcttaaa
    12481 accagtttct tttaacttgt cttttaataa atccattgta tttgccataa catcaccctt
    12541 actattttca attgataatc tttattaaat aatattgtaa ttgataattc attttttgtc
    12601 aagacattta aataaattta atttattctc ataatataaa tatataccca taaaagatat
    12661 attattcacc aaaattcata aattttttgg tatttttaat aaaaaacaaa aaaacagata
    12721 tatactataa atgcttttac aattaataag gtttaattaa agcaaatata ataaatatct
    12781 gatttttata tacaaactta attacttatc tagttaagtc catttgattc atcttcgtcg
    12841 taatttaaac cttcttcttc cattatagca gtatacacag ctacaacagt ttcatactct
    12901 tcatcacttt ccaaaggtat taatatttca ccttcttcat cctcatcaat tctaaagatt
    12961 aaagcttctt cagcttcttc atcatctaaa ggcattaata ttgcatactc ttttccttct
    13021 gcttctaatg ttaatattat ttcaaattgg ctttcaacgc cattttcgtc tattaaattt
    13081 attatatttt cttccataat gtcattcacc tcaaatttat ttttattttt attatttata
    13141 ttatatatca taagtattca attttatagt tcttttatga atttatttta aaatctaaat
    13201 atccttgcaa aattattact gctgccaaca tatctataac cttttttctt ttttgtctac
    13261 taacatctgc ttctaataaa gacctctctg ccgctactgt agaaagcctc tcatcccaaa
    13321 actctacatc aatacctgtt tctgctttta ccaattcaca aaacttgatt actttttctc
    13381 cttgtggacc taaagttcca ttcatatttt ttggtaatcc tgatacaatt ttatttactt
    13441 gtttttcttt tattattgct tttaactctt caatatcttt tttcttgcca actcttttta
    13501 ttgtttttac accttgtgca gttaatccca tcaaatcact cacagcaact ccaatagttt
    13561 tatcaccaac atcaagaccc attattcttc catctaacat agttcctcct ttatctattt
    13621 atattctata gattaaatac atattctgaa cctctcataa ctaaggtcac aggcttcctg
    13681 cttcatagaa ttttgcataa tcaatcataa aaattgcaat atgatttttt atgtaagcat
    13741 ccagtttaca ttaataatca tattgcattt tttatttaaa tcctatatga acagtaaaaa
    13801 attttttagt tgtttttttc aaatattatc atatttatat ttattattat tagtggtata
    13861 agtatatata tagatgctgt agatgcagca actattccag aatcattaac taaaagagta
    13921 attatacaac caaccataga tgcaataaac cctttaaaca agattggaaa attattcatt
    13981 atctttctaa agtgtctaga tggtttaaat ataagtactg caattactcc aattcctaca
    14041 agtaaaatat ttacccatac acttgtctgt gctagttgca tattcatttg aatttttctt
    14101 ccaaatgttt gtaatatctc tcctggtcca tttaggaaaa ttctctgtac aaacatacct
    14161 aaatgagatt cactacctga aagtatatct aaagctgcaa atgcaaaaac gacaactaca
    14221 gctgaaacag ctaataatac agctttctta aaatctattt taatgtcata tataagcatt
    14281 ataaacaaca aatatgctac acattctgat atagctccac caacatttgc acccattgaa
    14341 ggatatgcac tagttataag tattattaac gaaaaaatta caacaagtaa tttagatacc
    14401 ttcttataat gtaataatac tgctaagcca aatactgcac ttgctatagt tacaccttca
    14461 tattcatttc ctacaccata gtacctagct cctatgacag catcataact cattatgtta
    14521 ttttgcatta agtatgttcc aaatacagaa tctataacta tgactaaaat agtaagtgtt
    14581 gcaaaaaatc ccatgtgctt tatatcattg tccttaaata attttctacc taacaagtat
    14641 aatattagcg tagtcgcaaa tattcccaaa gatattgaaa ttggagtttt aaaattcatc
    14701 aaaggagcta acataaatga tagtggcatt attatgccta attttataaa ctccttaagt
    14761 actttgaata cacgctcttt atgtggcata taatttctaa agaaaatcgc aaacatggca
    14821 attacccaag aagcagatat tacgcctaca aagccattta taatagttga ccttattgaa
    14881 gatatagaca ctatcctttc ataaagtgat tttagatagc caacattgtc ctctttattt
    14941 actaaagtat aacttctacc taccatctct ttattttcaa gaccaaatgt acctagtata
    15001 tctactccaa catctaaatt ggcaacaatt ccagcctttc ttgttgtggc agattccagt
    15061 agacctttcc cttctccact aaattttatt attggagaaa gtcttctctt attcttataa
    15121 tctaggtcac ttggaaatgc acttgctata taaacagtgt cattttctcc aaccatagaa
    15181 aatacttgtt caagatattt atttatattt tttacaatat ttttcttcat tgtaccataa
    15241 gtattatcat ttagatttaa tctatacata tctaatctat atgtatctcc aagttctaca
    15301 aatatagcat cactattttt ataggatttt ttagtttcat ctagtaattt atcgtagtct
    15361 gtagatattc caaatggcat tttagaatct ttcttattaa tattatcaat attacctgaa
    15421 tcaatccttc catattggtc catagctact aaacataagt ttctattttt aactaattga
    15481 ttattttcaa ttatatcagc atttccaatt acagatgtct ttaaaccatt ttctgataaa
    15541 gtttttccta atgaccctaa atttgctcca tattctccat actcaagatt ttcatttata
    15601 gatctattaa tgcctaaatc atttatttct ttagcttttt gacctgttgc gacttcaaaa
    15661 gctttagcag tatcttggtc tactttttga aaattgatat agtcttcagt agttacacta
    15721 gctcttgttc ctgcacccat acttgcataa gaccttctat catcagtacc ttgatcccct
    15781 cttatattca tgagagctac atagcctctc ttttctatct catttttcaa gattggaata
    15841 ttcatcatac ttccaagatt ggtcctattc atatcaataa atatgacctt gcctttttct
    15901 tcagcaaaac catagctagc agttgataca aaaatcataa tgactattgc aaacaacgac
    15961 ataatctttt ttctcattta tttgacctac tttctattca gatagtgcgt gattacttct
    16021 tcaagtatct catctctttc aaatttttta attatagctc ttgcattttt atgactagtt
    16081 atatagctag aatcaccaga aaggagatac cctataattt gatttatagg gttatatcct
    16141 ttttcaacta atgctttata tacaaaatca atagtatctc ctacactcat cctatcttct
    16201 ggtattcctt caaatttcat agtgtagtct aaatcttttt ccaaactaaa aacctccttg
    16261 agtgaatgtt atttaacttg tgtcttaata acctcacttg catagcttaa agcttggtca
    16321 accttagata catcactagc tcctgcttgt gccatattag gtcttcctcc accttttcct
    16381 ccagctattt ttgcaacttc tctaacaata ttacctgaat gtattccctt atctaataca
    16441 tctttagtgg cagtaactac aaagttaact ttatcatcta ctacatttgc aagtactact
    16501 acaccagaac caagcttatc tcttagatta tctgctgttt ctcttagtgt atccatatcc
    16561 ataccttcaa atttattagt tatcaaatta actccattta cttcaacttt agaatcaaaa
    16621 atagaatcaa ctgattgtaa actcatctta gctttcatat catgcaattc tttagataag
    16681 tttttatttt cttctaataa agaacttatt ctttgaatta aattatcttc tttagatttt
    16741 aagtttacac aaacttcaga gataattcca tctctttctt ttaggtattc ataaacagct
    16801 tttcctgtta ttgcttctat tcttcttaca cctgctgcta ctccaccttc agataatatt
    16861 ttgaacattc ctacttgaga agtatttgtc aagtgagttc ctccacaaag ttctgtagaa
    16921 taatctccca tagaaactac tcttacctca tcaccatatt tttcaccaaa tagtgctgta
    16981 gctccttttt ctttagcttc ctttatattc ataatatcac attttatgtc taaagatgat
    17041 aaaataacat tatttacttt ttcttctatt acttttaatt cttcatttga tatcgcttca
    17101 aagtgagtaa tatcaaatct aagtctttct ggagttacta aagaaccagc ttgattaaca
    17161 tgttctccta atacttctct taatgcctta tgaagtaaat gtgtagcact gtgatttctt
    17221 gcagctgaca ttctagtttc tctatctacg agtgtcttta ctttgtcccc attacttatt
    17281 cttcctgatt ttattatacc tatatgtttt atactattat tagcaccttt tttagtattt
    17341 aatacttcta aaactaagtt ttcattagtt atcaatccac aatctccaac ttgtcctcca
    17401 ccttcaggat agaaagtagt gttatctagt actatagata ccttatcgcc ttcttctgca
    17461 ctttgaacta gctcatcatc tttaactata gcttcaatag ttccttcacc ataaatttca
    17521 ctatacccat taaatgtact gtcaactgta gattctaatt ttgatagagg gtcttccttc
    17581 caactttctc catccatatt tcctctagca tttctagctc tttctttttg cttttccatt
    17641 tcttcattaa aagcttcttc atctacagat agatgttctt cttctaatat ttctttagtt
    17701 aagtctatag ggaagccata agtatcatat agtttaaaag cttcttgacc acttaggaca
    17761 gtctttcctt cattttttaa tacttctata taagagttca atatttctgt tccttggtct
    17821 atagtttcat tgaatttttc ttcttctatt ccaactactt ttttaatata actttctttt
    17881 tcaactaaaa ctggatatgc tttatcattt acctttataa cttcatctat tagtttctgt
    17941 aaaaataatt cttttactcc taataactta ccatgacgag cagcacgtct aaggagtctt
    18001 ctaagaacat atcctcttcc ttcatttgat ggaagcactc catcactaac taagaatgtt
    18061 acagctctta tgtggtcagt tattattctt attgatatat cattttttgg attctctcca
    18121 tattttactc ctgttaactt ctcaactgct tctagtatag atttaattgt atcaacttca
    18181 aagatagtgt ctacgccttg cataatacat cccattcttt caagacccat acctgtatct
    18241 atattcttgt tttctaatag actataattt ccttcttcat ctctatcaaa ttgagtaaat
    18301 acatggttcc aaaattctaa atacctatca caatcacagc ctggtttaca gtctggattg
    18361 tcacatccat attcttctcc tctatcaaaa tatatttctg aacaaggacc acaaggacct
    18421 gtacctattt cccagaaatt atcatctttt ccaagtctaa ccattctttc ttctgggaaa
    18481 ttcatttctt ttgcccatat atcataagaa tcatcatctt cttcatatac agtcacccat
    18541 atcttatctt ctggtatatt tagccattct gtaacaaatt cccatcccca tttgattgac
    18601 tctcttttaa agtaatctcc aaatgagaag ttacccatca tttcaaagaa tgtagcatgt
    18661 cttgcagtta tacctacatt ttctatatct cctgttctta tacatttttg agatgtacac
    18721 attctcacac ttggaggtac ttctacacct gaaaagtagt ttttcaaagg tgccatacct
    18781 gagtttataa gtaacaaact cttatcattg tttggtacta aagaataact atttgctaca
    18841 taatgacctt ttgactcaaa aaattctaaa aacttacttc ttatttcatt taatcccatc
    18901 ttttccatat catgttcctc ctaataaatt ttattttttc gtttaaataa aaaacctcgt
    18961 ccctataaat aaatttatag gggcgaggac aatcgcggta ccaccctaat tatcatacaa
    19021 agtatgatat cttcggaaaa ccttagctat aacgtaacta ccgtcaatct ctacttaaat
    19081 cttctttata aaagataaat tgttcaagat tgctactaaa aggctgcttc aaatatcata
    19141 ttctaagggc ttccagcatt tccctctctc tgtaagacat caaatatttt actattcctt
    19201 atcactgtat ttgtttttta attaatatac taattatatt actaaatttt acaaaattaa
    19261 tcaatactat attttaatat acaataaatt tattttataa actttattta ctcttaatat
    19321 actttattgc ttattttttt aattttttag atgtttttat aaacttttct cgattatacc
    19381 tccacctact acaatatctc catcatacat tactacagat tgacctttag taatagctct
    19441 ttgagcttca tcaaaaacta ttttaatagt atcttctcct actctatgaa tagtagctgg
    19501 tgatggtttt gctgaatatc ttattttggc ttgaactctt aagggttctt ctaacgtatc
    19561 tattgatata aagttaacat ctttagcaat aagttccttt ttaaataaat cttcattgtc
    19621 tccaagaact atggtattat ttattggatt tatgtctatt acaaacattg gttttccaaa
    19681 agtaattcca agcccttttc tttggcctat agtataatat aatataccct tatgttttcc
    19741 aagtatattg cctttagtat ctacaaaaaa accttcttca atacgctttt tagaatgttt
    19801 ttttacataa tttgcatagt cattatcttt aacaaaacaa atttcttgac tatcaggctt
    19861 attgtgtact gccatcccca tttccttagc aatttctctt actctatctt tttcatagtc
    19921 acctattggc aataaagtat gctctagttg ctcttgtgta agattataaa gtgcataagt
    19981 ttggtctttt ttatctgtaa cagatttttt tagcaaatat cttcctgtac tttcatcctt
    20041 ttctatcttt gcatagtgac ctgtagctac ataatcacaa cctatttgtc tagccttctt
    20101 ataaaaatca tcaaatttta tatgtttatt acaagctata cagggattag gagtttttcc
    20161 ttccaaatat tcatctataa aataatctat tactttttct ttaaacactt ctctaaaatt
    20221 taaaacataa aatggtatac ctattttatt tgctaccctt ctagcatctt caacagcaga
    20281 taaagagcaa caaccacctt caatttcaac aacatcatca tcttgccaca acttcatagt
    20341 aacaccaatg acatcatatc cttgttgttt taaaagatat gcagctacag agctatcaac
    20401 tcctccactc atacctatca ttactttttt attcataaac aaatttcacc tcatcttttg
    20461 cattacttat tattatacca tatagagcaa gtattgtact tatttaaaat atagcttgtt
    20521 ctattttcta aaaaaataat catctttatt atttgaatct tatggattat tttgttaact
    20581 aattaacctc taaatcaagt taaaatactt aatttaggca ataaaaaata gccattttaa
    20641 aaacagctat tttttatgtc ctaattatat cattagtact tctttttaat cattgtaaac
    20701 ttctcataac acaatcactt atttttattc ttcttcttca acatcatggt catgagcatc
    20761 atctataaca tatccatcca attctggtat atggatattg ttcttttgag cataatctat
    20821 aagagctgct tttacagctt gttcagctaa aactgaacaa tgcatcttta ctggtggtaa
    20881 accatccaaa gcttctgcaa cagctttatt tgtaagttct aatgcatctt ttatagtttt
    20941 tccttttatc atttcagttg ccatagaaga acttgctata gctgaaccac agccaaatgt
    21001 tttaaactta acatccttta ttacatctcc atctatgtct aaaaatatct tcattatatc
    21061 tccacatgta gggtttccaa cttcacctac accacttgca ttatcaattt ctcccatatt
    21121 tcttggattc ataaaatgtt ccataacttt atcactatat tgcattatct tttcctccta
    21181 ctcaatttat gtctattttt tatgctttat ctttctgtct attttactaa accttgtttc
    21241 ttcgcatcat cataaagtgg tgacatgctt ctaagtcttt ctattacttt tggtaagttc
    21301 tctagtatat aatctatatc atcatctgtt gtaaaatctc caactgtaag tcttaatgaa
    21361 ccatgtgcta tttcatgtgg taaacctatt gcaagaagta catgtgaagg gtctaatgaa
    21421 ccagaagtac atgcagaacc acttgaacca gcaattccta acatatctaa taataaaagt
    21481 attccttcac cttctataaa ttggaatgca aagtttgcat ttcctggcaa tctattttct
    21541 aagctaccat ttactcttgt gtaaggtatt ctttctaaca caccatctat taacttattt
    21601 ctaagacgag ttaaagtttc tacatgattt tgcatatttt ccttagctaa ttctgctgct
    21661 tttccatatc caactattgc aggtatattt tctgtaccag ctcttttgct tttttcttgt
    21721 gctccaccat ggacaaagtt atgaagtctt acaccttttc ttatgtataa agctcctaca
    21781 ccctttggtc catatatttt atgtgaagac atactcatca aatctattcc taattcttta
    21841 acatctactg gaatattacc tgtagcttgt actgcatcag tgtggaataa tatcttatgt
    21901 tttttagcta tttcggatat ttctttaata ggctgtatag ttcctatttc attatttgca
    21961 aacattatgc ttataagtat agtagtatct tttattgaat cttctagttg tcttaagtct
    22021 actcttcctt cgctatcaac atctaaataa gttatttcaa atccatgatg ttttgctaag
    22081 tattcacatg tatgaagtat tgcatggtgc tctatttttg aagttattat atgattacct
    22141 ttgtttttat tagcataagc tacaccctct agtgtccagt tatcactttc tgaaccacct
    22201 gctgtaaaat atatttcact aggttcagca tttataaggg ctgctacttg ttctcttgct
    22261 ttatccaaag catcttttgc ttctcttccg aatgtgtgaa aactagatgc attaccaaaa
    22321 taatctgtta ggtatggcat cattgcatcc aatacttctt ttttaatagg tgttgttgca
    22381 gaataatcca tatacaatct tcttttttcc attacattca tctccaaatc tattttttaa
    22441 tatttgttat atcattttct aatttatttt tgttatagtc atttatcata tctttaagag
    22501 taattgaatc tataacatct tctataccct ttttcatctt ttcccaaact atttttgtta
    22561 cacacatgtt tgagttttca catacatctt catctagtac acagtcagat aatgcaactg
    22621 gtccttctaa tactacaagt atatctccta ctgttatgtc ttcagcattc tttgaaagca
    22681 aataacctcc ttgagcacct ctcacacttt ttactagacc cgattttttt aaagatgaaa
    22741 atatttgttc taagtactga tctgatattt tttgcttctt agcaataaat tttaatgata
    22801 ctggtccatt gtcttgattt aatgctaatt caaacattgc ttttagtcca tatctacctt
    22861 tagtagacaa cttcatatat tatcacctct atatagaaat ctaattcata ctgttttagt
    22921 aggaattact ttcaagataa ttttattata ccctactaat tttgtcaaga ttaattttaa
    22981 ttttttattt tgaataaatt ccccattttc caatgttttc aaatcctagt tttttatata
    23041 tctttccagc ctcttcatta tcataaaaca aacaaggaat tttgttttct ctcagtaact
    23101 ctgaacatag tttaattagg cattttgtag caagtccttt agctctatat ttaggatgtg
    23161 ttcctactcc aattatcatt gcatgagtcc tattttctga agtactcttt gccattgaca
    23221 ccacctgttt gttaatctct atacaatatc ctctacctgt ccttagtcca ttttttaaat
    23281 tctctaaagt tgtactgtga aattcatcta ttaagtcata aagtttcact gtctttttta
    23341 gatttccaag cctaattctc ttaacctttg cattacaatt attatctatc aaaaactttt
    23401 tagtttgaag tttgcaaaaa tcaactacct ttaaattatt aagtcctaat ctctttgcta
    23461 aagtatctac agcacatatt ttcccagata tttcttcgta attagtatta cgaagtagat
    23521 tataaaaacc ttcaacatca tactctccat cagaataaaa catcataaat tcaaaatatt
    23581 taagtaatat tgcctttatt tcaccatgtt cacccacgtc tgcccatata tttaaaaaat
    23641 tatttccata tccatatctt tcaatatccc ctattataaa caaattgtac tcaggttctt
    23701 tttttaagta tttcataact ctactgtgat aagttgagtt aagttttata atcataatat
    23761 ctacccccga tacataaaat tcagtctaaa aatcactatt tagatattct aaatattttt
    23821 ttatattttt ctcagtttta ttttcttgtg gtatgtaata aatactatct tttatgctat
    23881 ctggtaaata ctgttgcttt acataatgat ttttaaaatt atgaggatat ttgtatccac
    23941 aacccctacc tatttcttta gaaccagaat agtgagcatc ctttatacta tttggtatat
    24001 ccttaacaaa ctctttatcc agttcatcca tagctttcat tattgctata catgcagaat
    24061 tagactttgg agatgtagcc aaaagaatag tagcttctgc taatggtatt ttcgcttctg
    24121 gaaatccaag ttgcatagct gagtctacac atgcctttac aattacaata gcatttggat
    24181 aagctaaacc tatatcttca ctagctatta ctaaaagtct cctacatata cttatcaaat
    24241 ccccaccttt tataagtctt gctagatagt gtatagatgc ctgtgggtca cttcctctta
    24301 tagatttttg aaatgcactt aaaatatcat aatgactatc accatttttg tcatagttta
    24361 taattttatt aaatgtagat gcccttatta catctgaatc tatataagtt atattatcct
    24421 tatttggttt agttgaatag actaccactt ccaaaatatt taaggctctt ctcatatctc
    24481 catcagaaag aattgctata tcttcaatag cattattatt gcactcaata tccatatagc
    24541 tatcttcatt taatacttca acagctcttt tcagtccttt ttctatatca agtttttcta
    24601 aaggtttaaa ttcaaataca gtagacctac ttaaaagagc tttatataca taatgatatg
    24661 gattttctgt agtgctagca atcaaagtta tactcccatt ttccataaat tctaatattg
    24721 attgttgctg ttttttatta aaactttgta tttcatctat atataatagg actccattta
    24781 cattatttat acttccaagc tctgatatca ccttttttat atcttctaaa gatgaattag
    24841 tagcatttat tttatagaag cttttattgc ttctttctgc tattatttcc gctactgtag
    24901 ttttaccaac accaggagga ccaaaaaata tcatatttgg taaaaaatta gattgtaata
    24961 tcttagacaa tatttttcca ttacctatta tatgactttg acctattaca tcattcattg
    25021 tttttggtct tattttatct gctaaaggca ttttctcact cctttttaat atacttatca
    25081 ttaccttaat aattcgtcta aaaaaggtat ctatttgaaa tagatacctt ttctaatcta
    25141 aacctataaa atatagatta ttgattgttt tttaattttt ttaattcatc tgtaagtttg
    25201 tcattaagta ttcttatatg agtacctttc atacctaatg atcttgattc tatcacacct
    25261 gcactctcaa attttctaag tgcatttact attacagacc tagttatacc aactctatca
    25321 gctatcttac ttgctacaag tagaccttct tttccatcca attcagcaaa aatatgttca
    25381 actgcttcaa gctcggagta ggacagagtg cctattgcca tttgaactac agctttcttt
    25441 ctcatttctt cttctaattc ttcacctatt gctcttaata tttcaagacc aacaacagtt
    25501 gcactgtatt cagctattac taaatcatca tcattgaatg aatttgaata tcttgaaagt
    25561 accaatgttc ctaatctttg accgcttcct aatattggaa ctactgtagt atatttttgt
    25621 aatcttccat gttcttcagg aaagatttct aatatcttct caccatttaa attttctaat
    25681 gtttcatcaa tttttaacac attttgagta tattcatctg aaaatttctt ttgcttagta
    25741 tactcatctt ctataactga actgtcttga acatcattta aatgaagacc tagtacttta
    25801 ccttttgcac ttactacata aacattagaa cttaaaacgt cgcccaatgc tccggccagt
    25861 aaatcaaaag agacactgct tccaccactt gtttgtaatg ttttatttat tttccttgtt
    25921 ttttgtaaca cttcacttgc catttaatca tctcctcatg aaatttaggc cttttaataa
    25981 ccttttatat tttctaatat tattttcaca tttaatgaaa atttattttt ctattagttg
    26041 tttacacttg aaagattatc atagattgga acctttttca agagaattat gtgatttttt
    26101 tcaattttca ctcttttccg tcattatgct cttttgtctc tttattacat tctttattag
    26161 aacacactgt ttttgtttca gatttagtca ctttttcaac catatatgaa ccacacttct
    26221 cacaaaactc tcctgttggc ttattccaag ctacaaagtc acagtttgga taattagaac
    26281 acccataaaa agctttacct cttttagatt ttctaagtat aatatctcct tcttcacatt
    26341 ttggacattt aacaccaatt ttattcataa ttggtctagt tgttttacaa tcgggatagt
    26401 ttttacaagc cataaacttt ccaaatctac catatttgat aaccatatta gacccacagt
    26461 tctcgcaaat ctcatcagtt tcttcatcca tatttacttt ttctatattt tcaagagctt
    26521 cttcaatagc ctctttaagt ggtgcatatg attctgatac aatctttttc caatctgtat
    26581 tgccttcttc tataacatca agtttacttt ccatatcagc agtaaaatct acatctataa
    26641 atttatggaa attattttcc aaaatatcag taactattat tccaagttct gttggacata
    26701 gacttgttcc ttgcttttca acatactctc tattcagtat agtagctatt gttggagcat
    26761 acgtacttgg tctacctata cctagttctt ctagtgtttt tactaaacta gcttctgtat
    26821 atcttgctgg tggttgtgta aaatgttggt ctggcaatat attttctata tttaatatat
    26881 ctcctttttc tatacttggt aaaattttgt cttctctttc tgaaaaatta tataccttgg
    26941 tataaccatc aaatttcatt tttgaaccag ttgccttaaa tacaatatcg cctattttac
    27001 attctacatt tagtatatca aacactgaat cttccatttg actagctaca aatcttctcc
    27061 aaattaaatt gtataacttg tactggtctt tgctcaatga ggcttttata ctctctggag
    27121 ttctatcaac tgaggttggt cttattgcct catgggcatc ttgaaccttt ttagtatcct
    27181 ttttttcttt tttatcacca gtgtttttat agtagttttc tcctaaattg tttacgatat
    27241 aacccttagc tttttctttt gcttcatcag atattctttt agagtcagtt cttatatatg
    27301 atataagacc aacagtacct tcaccttcta tgtcgatacc ttcatataat tcttgtgcta
    27361 tcatcattgt ttttttagta gtaaaaaata acttatttgc tccttcttgt tgaagcatac
    27421 ttgttgtaaa tggttttgga gctgatttac gtcttgattt agattctatc ttctcaacct
    27481 ttaaatcttt accttccatt tcttttagta tttcatttac agcttcttca ttttctaatt
    27541 caatcttttc atcattttta ccataaaatt tcaaagttat atctttgtct tttactgtct
    27601 tagcctcaac atctattgtc cagtattctt ttggaataaa tgcctttatt tctttttctc
    27661 tatcacaaat taactttgtt gtaacagatt gcactctacc tgcactaaga ccttttctaa
    27721 ccttttgcca taatatagga cttatttgat aacctaaaag tctgtctagc actcttcttg
    27781 cctgttgtgc atcaaccaaa tttatatcaa tatttctagg atgtttaatt gcttttttaa
    27841 ttgcatcctt agttatctca ttaaactcta ttctacaatc ctcagattca tctaaattta
    27901 gtatatgagc taaatgccat gatatagctt caccttctct atcagggtca gttgctagat
    27961 atacttgttt agatttttta gcttcttttt ttaattcttt tattacatcg ccttttcctc
    28021 ttatatttat atattgaggt tcaaaattat tttctatatc tacacctaat ttacttttag
    28081 gtaaatctct gacatgacca actgatgctt taactgtgta atgacttttc cctaaaaatt
    28141 tttctatagt tttagctttt gcaggcgatt ctacgatgac taatgttttg gccataaaac
    28201 catacacccc cactacctaa tcttaattta tacatttaaa ctgtacgttt tattattcat
    28261 ttctactact aactcattta gtactaattc attgattatt gaatttacat actttatttc
    28321 tataccagta taatcacaaa tactatctat atgcaagttt ccttctcttt ttatagcttc
    28381 aattatttgc ttagacttgg catttagact tatattatca tatttttgat ttattttttt
    28441 atcaataata ttaaatatgt tatattcatt taaaatatca tctatatttt caattaattt
    28501 tgcaccttct tttataattt tatgacaacc tctactcatt tgagaattta tattaccagg
    28561 tattgcaaaa acattttttc catgttcaag agcaaaatct actgtgatta gtgcaccact
    28621 ttttattgct gcttctacaa caactactcc atcacttaaa ccactgataa ttctatttct
    28681 attagaaaaa tggtacgggg ctactgtcga gttaatatta tactctgata ataataatcc
    28741 cccattttct aatattttat ttgataaatg taaattttgt tttggaagag gattgtctac
    28801 tcctgaacca agtactgcta tagttttaga ttttccactc atacaaccta tgtgcgagta
    28861 tgcatctatt cctatagcta atccacttac tatattaact ccatattggg atagttgaca
    28921 acttattgtt tttgcacaat taattccata ggctgtaggt tttcttgaac ctactatagc
    28981 tatattaaaa ttattattaa ctaaacctat gtcacctttg tagaataata actttggagc
    29041 gttgtatata ttctttaaat tttcaggata ttctttatca tatttacaaa tatatttaat
    29101 cgcttttttg taaagtagtt cttttatatt ttctaaataa gcgtaacctc tatattttac
    29161 tatatttttt ctaatattca agcttatatt ttttaaatta tatatttctt tttcagaaaa
    29221 atcaaatata tcttcaatat ttactatttc attttctatt atttcaattg tttttgttgt
    29281 tattccacca attgatttta accataaata agtatctctt ttttccataa aatccacctc
    29341 aaaaaactat ttaaaataac tataataagc ctttctaaat gaaaaagctt ctataataca
    29401 ctgtgaattt atcaagtctc tttcttctaa atctgctatt gttcttgcca tttttaaaag
    29461 ttttgtataa cttctactgc ttaggttgta tttattaaat atcatctcag ccgtttttga
    29521 agcttctttt tccagtttac aatatttaaa taaattgtaa gctttaattt cactattatt
    29581 ttttatatta tctttcttaa atctatttat ctgtattttt ctagcatttt ctactcttct
    29641 ctttattttt tgagagcttt cttcttgttt taaagaatta aaatctgaat attttataga
    29701 attaacttct acaaaaatat caaacctatc taacaatgga cctgatattt tatttatgta
    29761 tctatcaatt tcgttacttc tacatctaca ctctgtctct gacatataat aaccacatgg
    29821 acatggattc atagctgcaa ctaacagtac tctacaagga tattttgcac tgtactttac
    29881 tctggatata tttacatatc catcttctat aggttgtctt aaagtctcaa gtatttttct
    29941 atcaaattcg gctatctcat ctaaaaataa gatacctcta tgtgctaaag caatttctcc
    30001 aggtcttgca tccattcctc ctccaattaa agattgcttt gtagttgtat gatgaggtgc
    30061 tctaaaaggt cttttatcaa ttattccttt actttcattt atcataccca atatactata
    30121 aaccttactt acttctatca tttcttctat acttatatct ggtaaaattg tccttactct
    30181 ttttgcaatc atagtttttc ctgagcctgg aggtccaatc ataaacatat tatgatttcc
    30241 agctgctgca atttctgcac tcctctttac aaaataattt ccttttacat ctttaaagtc
    30301 ttcatcatat ttactttctt cattattaat cttagtatat ttgcaatcta tataactatt
    30361 atctttttct aattctccgt tctcttttct tgacttatca tccaaaaaag acattatact
    30421 tactttatcg acttttatct cttcgtttaa aaaatcgaca cattctttta aacttttaat
    30481 aggaattata tcaatttcat ctactaatag actttctttt atgttctcaa ttggtataaa
    30541 aatacgtttt atgttctgag tttttgcacc cataattatg ggcaatatcc ctcttacttt
    30601 tcttattttt ccatctaatg ataattctcc tataaacata ctttctctta tgtaattttc
    30661 atcttttttt attaactctc ttaaaaggcc tatacttata gataaatcta aaaaagctcc
    30721 ttcttttttt atatctgctg gtgataaatt tacaactatt ctcgtgcttg gaaatttata
    30781 tccactatta agaattgctg actttactct ttctcttgct tctttaattt ctttgcctgg
    30841 aagaccaaca atattaaaac taggtattcc attgcttaca tctacttcaa ccttaactaa
    30901 aaagctgtcg attcctacta agttgcttga attaataata cttaacatat ttaatccttt
    30961 ccaaaaacat tatctttttt aaaaagcatt cattatgtga tttattcttt tttctaacaa
    31021 atagacctct atgatgtcaa atcttatggg agtattattt aagttatttt ttataatata
    31081 gtattttgcg acaccaatta tcttttttct ttttttaaaa tctacagatt cacaagggta
    31141 gccatacttt atgttagacc ttgatttcac ctccacaaat acaacttcat ctcctatatt
    31201 tgctattata tctacctccc ctatccttaa tctataattt ttttctagta tctttgcacc
    31261 ttttgatagc agataattaa ctgctatttc ttctccaaaa tcaccttttt ctttattatt
    31321 cattatctac tccaatatat taaattttta cttttaaatt atcgtcagta tacaatttaa
    31381 ctattcaatt ttttgaaatt gattttattt ccattgatat taataaatac ttctattgac
    31441 tttattcttt tatgatatta tgctagtttt tataatgaaa gtttattttt atggaggaat
    31501 gtctttaatg aacagaaaaa aaacatttat ttttatacga tatttatc
//
LOCUS       DAGQWR010000033        31021 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.033, whole genome shotgun sequence.
ACCESSION   DAGQWR010000033 DAGQWR010000000
VERSION     DAGQWR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.033"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            305..703
                     /locus_tag="MIW95_001300"
     CDS             305..703
                     /locus_tag="MIW95_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3150642.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMMILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(920..1129)
                     /locus_tag="MIW95_001301"
     CDS             complement(920..1129)
                     /locus_tag="MIW95_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150643.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(1161..1370)
                     /locus_tag="MIW95_001302"
     CDS             complement(1161..1370)
                     /locus_tag="MIW95_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3150644.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            1669..2088
                     /locus_tag="MIW95_001303"
     CDS             1669..2088
                     /locus_tag="MIW95_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3150645.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            2316..2771
                     /locus_tag="MIW95_001304"
     CDS             2316..2771
                     /locus_tag="MIW95_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150646.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(2996..3256)
                     /locus_tag="MIW95_001305"
     CDS             complement(2996..3256)
                     /locus_tag="MIW95_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150647.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(3519..3872)
                     /locus_tag="MIW95_001306"
     CDS             complement(3519..3872)
                     /locus_tag="MIW95_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150648.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQAKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGRKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(3889..9066)
                     /locus_tag="MIW95_001307"
     CDS             complement(3889..9066)
                     /locus_tag="MIW95_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HBY3150649.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIDDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRATFTKVNIDSVKDVVTYNQSVFIIKMDGTAHACGLNSNGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGYNGVGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFAKVNVENIKDI
                     KCGSQFNFLINGSKEIFVSGCNLAGQLGSFFHTTFLYEFSNVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNVKLSDNFQDYKKIELTDSNMFIVMNDGTLYACGLNNYGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGNSTFVLKNNGTLYSCGLNSNGQLGLRDEVNRNIFTKI
                     EIENVKDFCVGSNYVIALNHSKEVYGWGNNPYNNIEKTSNYPYKQGISNIEKIAAYDY
                     SVYMINSEGKLYVSGYNYNYQLGKGNNSNQSKALVSQCRTNSTSSTSNGLRTLPKITN
                     VFPFYDGCAIIDEGGYVYLTGYHGYLRTLNSSPSISDYSRYGTFIEATNSNHNTYFIQ
                     ETDFSGIEKVIGMSNNILFFKKGSSYITGYPKTFGSTITGHRSYTSINSESSNLGSNF
                     IIYHSNSKLYGKGIANSGQFGNSTNIDGTSNYDTGLKDIKDIIVKGNTVVVVDKNNNI
                     YVTGMNQNNKLGIGEYNNEPVKKFTNITEQSNSFIFMDDIKEITTSRNTMFIVKNDGT
                     AYATGNNSSGQLGLGDTINRNKFTQINLDNIKKISTSIDGNTTFAIRNDGTLYSTGLN
                     TKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDDNTLYSCGENTHGQLGLGS
                     ESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCCGYNNNSQLGMGNTTDQYS
                     FIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGLNSNSNQSSFSEIPISNVV
                     KVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIKKIASKAKDIGMNYRCGHY
                     VSDNGDLYGTGFNNNGQLGVGDVTKRDTFIKTNTRVKKILPLEYANIAIKDTNDIYIC
                     GLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIKNRHNFILLNNKIVIPTTK
                     DIDYGLVLGNLYKGDLYTELPYEDIKEVSISKTHIIILLNDGTMYGCGTNYHGELLQD
                     LSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIGKNSEYQLGIGHNNPVTEL
                     QRITTISSCKEVHCGKNYTLVVTTGNELFVQGYNDKGALGLGSDSENTIIKFFTKALT
                     DIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEFLKEFTIVPISEDVNTVKD
                     VLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNKDISRIEMLINGESVKSVS
                     DLTTEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFIFKESTGNSIIKDSYVMIG
                     NRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINKTKVQVKINKSDLFKDMKL
                     VEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(9088..10074)
                     /locus_tag="MIW95_001308"
     CDS             complement(9088..10074)
                     /locus_tag="MIW95_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBY3150650.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGDTDILTTDSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLANGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLSKYSYEIR"
     gene            complement(10075..10773)
                     /locus_tag="MIW95_001309"
     CDS             complement(10075..10773)
                     /locus_tag="MIW95_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBY3150651.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSEMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(10770..11822)
                     /locus_tag="MIW95_001310"
     CDS             complement(10770..11822)
                     /locus_tag="MIW95_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBY3150652.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGENNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(11815..12243)
                     /locus_tag="MIW95_001311"
     CDS             complement(11815..12243)
                     /locus_tag="MIW95_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBY3150653.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(12243..12569)
                     /locus_tag="MIW95_001312"
     CDS             complement(12243..12569)
                     /locus_tag="MIW95_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBY3150654.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(12585..14114)
                     /locus_tag="MIW95_001313"
     CDS             complement(12585..14114)
                     /locus_tag="MIW95_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBY3150655.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(14396..14818)
                     /locus_tag="MIW95_001314"
     CDS             complement(14396..14818)
                     /locus_tag="MIW95_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150656.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEVATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(14818..17271)
                     /locus_tag="MIW95_001315"
     CDS             complement(14818..17271)
                     /locus_tag="MIW95_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150657.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMAKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKRVEKRPYQGSINLKDMVSSAMARILPKLMLFKNT
                     FWSGVIAIKDMASSIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKTSQKDVDKYYDSLVNMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSSKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(17271..17438)
                     /locus_tag="MIW95_001316"
     CDS             complement(17271..17438)
                     /locus_tag="MIW95_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150658.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(17465..17911)
                     /locus_tag="MIW95_001317"
     CDS             complement(17465..17911)
                     /locus_tag="MIW95_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBY3150659.1"
                     /translation="MNENGLSKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(18025..18453)
                     /locus_tag="MIW95_001318"
     CDS             complement(18025..18453)
                     /locus_tag="MIW95_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBY3150660.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(18467..19531)
                     /locus_tag="MIW95_001319"
     CDS             complement(18467..19531)
                     /locus_tag="MIW95_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBY3150661.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTEEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(19536..19979)
                     /locus_tag="MIW95_001320"
     CDS             complement(19536..19979)
                     /locus_tag="MIW95_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150662.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTITLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(20228..20668)
                     /locus_tag="MIW95_001321"
     CDS             complement(20228..20668)
                     /locus_tag="MIW95_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150663.1"
                     /translation="MENKKDILFKETDERLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(20773..20970)
                     /locus_tag="MIW95_001322"
     CDS             complement(20773..20970)
                     /locus_tag="MIW95_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3150664.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            21139..21459
                     /locus_tag="MIW95_001323"
     CDS             21139..21459
                     /locus_tag="MIW95_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBY3150665.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            21541..22047
                     /locus_tag="MIW95_001324"
     CDS             21541..22047
                     /locus_tag="MIW95_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150666.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            22748..23503
                     /locus_tag="MIW95_001325"
     CDS             22748..23503
                     /locus_tag="MIW95_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBY3150667.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(23646..24074)
                     /locus_tag="MIW95_001326"
     CDS             complement(23646..24074)
                     /locus_tag="MIW95_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBY3150668.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(24495..24695)
                     /locus_tag="MIW95_001327"
     CDS             complement(24495..24695)
                     /locus_tag="MIW95_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBY3150669.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(24878..26047)
                     /locus_tag="MIW95_001328"
     CDS             complement(24878..26047)
                     /locus_tag="MIW95_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBY3150670.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSEDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            26283..27104
                     /locus_tag="MIW95_001329"
     CDS             26283..27104
                     /locus_tag="MIW95_001329"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBY3150671.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(27180..28568)
                     /locus_tag="MIW95_001330"
     CDS             complement(27180..28568)
                     /locus_tag="MIW95_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBY3150672.1"
                     /translation="MLRSKKSNNKKVEKLRTIFIKYLSLFFVMTISFALLLMLSFSVL
                     LSSGVILPANYSEKQFDKYKEKIISSEKAIEDIIPNLYEYGVYTLDGNLISGTFNKKE
                     SKQVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKLVSEYSSPVLRAYLPKPEMLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNILF
                     SMDKMKLALKNSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAINMSEFIKDVLLQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVSNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(28712..29470)
                     /locus_tag="MIW95_001331"
     CDS             complement(28712..29470)
                     /locus_tag="MIW95_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBY3150673.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(29472..30188)
                     /locus_tag="MIW95_001332"
     CDS             complement(29472..30188)
                     /locus_tag="MIW95_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBY3150674.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWAGKMIYCIICCSIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLTNMFFT
                     IVGVAVFSTTEMWYVFPYSIPSRLMVVVLHILPNGLPVPEGSELLNNAVILPGVLISV
                     ILFVLFALVTRAWFNNREVK"
     gene            complement(30190..30897)
                     /locus_tag="MIW95_001333"
     CDS             complement(30190..30897)
                     /locus_tag="MIW95_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBY3150675.1"
                     /translation="MNNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRKLIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVIGYQGEVSKEQN
                     LEKLFMKVVAENRKRGE"
BASE COUNT        10032 a         5274 c         3356 g        12359 t
ORIGIN      
        1 ttatcttaat ctttttctct tttacagaca atcaaaaagc aatcttagtt tatcgaaata
       61 aatttataca atctattcat acaccccttt acatttttat aacaattaaa tctattgtta
      121 tatatacaaa tcaaaataaa ccatatattt tcaactataa tatgttaaaa tcatattatg
      181 agcatattaa tttaagcaaa atgtcattta gactttaatt tagtagtttt atattataaa
      241 tttatatatt tttaatttca aatgtcattt agacattact cataaaaaga aaggagtgta
      301 ttttgtggat actttaggaa aaagaattgc ttatttgaga aactccaaaa aacttactca
      361 gcgcaaactt atggatattt taaaatttga aaacttaggc aagtatgaaa caggagatag
      421 aaaacctaat tgtgatatac taatgtcaat agctgattat tttaatgtta cgactgactg
      481 gcttctctat ggtaaagaga aggtaaatgt caatagtagt gtaaaagaag ataaagaaga
      541 ttacttacat gttactaatg atgaaatgat gatattaaat ctatacagac aactaaacga
      601 acgtgataaa ataaaaatag aaggaatact tgaacttaag atatctgaat ataaagactt
      661 aaaaaagcat tcctctaata ataatgaaga taaaatggtt taaacataca taatttgata
      721 cgattttaca tcatctatta accttctttt tttgtagtat attactaaaa tatggatatt
      781 ggattattta aaaacaagta tagtctaatt tcaagcacat ttataatatt tagattatct
      841 tatccatatt ttagtttaag tactttgaaa aatataatac atttctatgt ataatttata
      901 ttcctaagaa atgcattact catgttttta attcttccaa catctttcta tacacaattg
      961 tacatccttc ataattcccc tctttaagaa ggattgaaca tgtactagaa tatttagtta
     1021 aagtatttaa tctttccata tttaagcttt ttatattact aagtgctgta ataaaatcat
     1081 caattgctct acaacactct ataaccccat ctgaatccaa tttgcacaca agaatattca
     1141 cctactttat atactattta ttaatcatct tctgaatatt ctatattaag taattgataa
     1201 attgatgatt tatatttttt acctgttctt cttccattta aaatagctga caaatagttt
     1261 tcattaacac ctattttctt cgctaattcc ctttgagtca tatttaattc aataagtctt
     1321 tgcttgactt gcactcccaa aaaaggtatc catttttttc gagcatccat ttatcaactt
     1381 cctttctaag tgatttatat ttacttttat attactttag cttacgcacc ccctctattt
     1441 agtatatatt tttaatatgt acacatatta agtatataaa tttatttaat actaaataag
     1501 attttttgat tactttgtgc taaaattagg atgtaaattc gtatcattaa gttaattata
     1561 aagtcatttt gaccttaagt caatggtttt tatgtcattt tgacctttaa tttgaaacta
     1621 tttacatctt aaaaagtctt tttgacatta attgaatgga gagtgtttat ggatacttta
     1681 ggagaaagaa ttgtttattt gagaaaagca aaaaacctta aacaatatga actagaagaa
     1741 atgttaggct gtgacaactt aagtaaattt gagagaaaca ttagaaaacc aaattatgaa
     1801 atattaaaat ctatagctga gatatttaat gtttcagtag actggctatt aaatggagat
     1861 aatctatcac ataaatcaga tttaatttgc gattcctcct caaactatcc acttaatagc
     1921 ataaactcta atgaaataaa gcttttaaac aactttagaa agttaaatga ttatgataaa
     1981 gccaaaatag aaggtatgat tgaattaaaa cttcatgaat atgaaaaaga gaaggattta
     2041 ggaaaaattg agtataacaa aaataaagat gagaaaatag ataaataatt tttatgttgc
     2101 ttttgatacg attttacatc atttctttgt tctaatattt ttatttcatt tattttcttt
     2161 tttataattt tatgataaaa taattagtat attttgcttt acttgaatgt aattatttat
     2221 acaaaagtca aacataatat aaatgtagta aatttttagt ttcaagtaat atctcgttat
     2281 tataactaaa tatttattaa aattaggagg ataaaatggg cttaagagac aaattcgcgc
     2341 aatcatttgc tagatcaaaa actatgagtg gccctgagaa aaaggctaat gaaataatgg
     2401 gtaaattgct tcttaagaaa gctatattac ctatcgtttt aatgttcgtg ataataatcg
     2461 ctggagctat gcttaaaatc aattcttggg ttacacttgg cataaattta gttattgctg
     2521 ttggtgcttt cttctacata agaaattcta gtaaaaagta tcagaacttt aaaccttatg
     2581 ttggcaattt gattagttta gagaaaaaag gtaaaaaaga gtacgttgct attataaaac
     2641 aaggtaagtt accagttaag ttacaaatag cttatggtgg tgaagacctt gaacatgtta
     2701 agaaaaatca aatggttcag ataagttata atccagatgc taaaatagct attttggtta
     2761 atagacaata atttaataaa aaaaaggagt tgtcttaaaa ttgatttttt ctaatcaaat
     2821 ttaagcttaa ctcctttttg aatttttata tgttattttt atatattatt tttattactt
     2881 tttttaatta atattttaaa atataaaatc cttctcaata aaatagggca ctatctacaa
     2941 tactacttta tcttttccta ataatatata atctggtgct actgctactt tcatgttata
     3001 caccgttttg taacatacct tttatatcag taacatcttc cttaatttcc tctacatctg
     3061 ttttcattat tcccatttga acaagtatat ttttgtttat ttcttgttgt tgtgtagata
     3121 attctataaa attttctaca gtttttttat acatgtctct atcttctttt ttctcctgca
     3181 tagtattttt aaataataga gcacatagta tacctattgc ccccagacta ctcaattcgg
     3241 ttattaattt atccattata gcctcctatt gtctaaattt tttatactta tagctttaat
     3301 ttttgttata ttcttacatc atgcaatatt atattttcag gattatcaat ttaatcttct
     3361 gtactttaaa ttgtaggttt aatttataga ttgaataaat aataaatata tatacaattt
     3421 ctaatataac aaaaaataag tacaaggctt acttattaaa gttaagacct tgtactttac
     3481 ataaaaaaaa taactaatac atagttattc taaatttact attcaactgg tatttctaat
     3541 tctttacatc taattctaac ttgctctcta attactgctg gtacttcttc tattgttttt
     3601 ctgcctctta aaattaaagc tacatatact tctaccattc cactctcacc tccagatttt
     3661 cttacactaa aagtgcttat attacttgct tcattgacag aactttgact taacaacatc
     3721 tcatatattt ctgcatttgc tataaaatta tttgttatat catcatcttg ctttgcttga
     3781 cctatactta tgtctgaaat gtctttatac tgcatttcta cttttctacc ttgtttgatt
     3841 tgttctattt tatttttatc gaatttaaac aatttttaca cctccatttt aagcttgtgg
     3901 ctcgttgttt tcagcatcat atctaaaaat catagatgga cgttttatag ctgtccattt
     3961 atctcctttt tctactatga ttttaggctg agcacttttt atgttggctt cttctaattc
     4021 atagatttct tgataacttg agtctgattt tttgatttca actagtttca tgtctttgaa
     4081 taagtcagat ttatttattt ttacttgaac tttagtttta tttattaatt gatatatagg
     4141 gtctccaaga tttaaatctt cttcaagtcc tctatctagt gtaattgtaa tatcttgttc
     4201 attagatgtt gtattaacta ccttgtacat tctattacct atcataacat aagaatcttt
     4261 aattatagaa tttccagtac tctctttaaa aataaataaa gatgcatata aatcatcacc
     4321 tttacaataa gctctaaata gtatcttatt ctctcctatt ttaattttat ctggtggtac
     4381 ttcaaaggat attttttcag tagttaaatc acttacagat tttacacttt caccatttat
     4441 aagcatttct attctactta tatctttatt agggtcttgt atttttatct taattgatga
     4501 aattgatttt tcagtaattt ctatagcagc atttgtcgtt cctacttctg atataatata
     4561 taatgtattg tctgttgcaa gtacatcctt tactgtattt acatcttcag aaataggtac
     4621 tatagtaaat tcttttaaaa attctactgg ttgttcaatt ttatatacat ccctattttt
     4681 tccagtaacc catactgaat tatcattttt aagtactaat atatggtcac ttccataaga
     4741 ttttatttct cttatgtctg ttagtgcttt tgtaaagaac ttaattatag tattttcact
     4801 atcgcttcct aatcctaaag ctcccttatc attatatcct tgtacaaata attcattacc
     4861 tgtagttact actaatgtat agtttttacc acaatgtact tctttacagc tagatatagt
     4921 tgtaattctt tgtaattcag taactggatt attgtgacct atacctaatt gatattcgga
     4981 atttttacca atagaccaaa gactatcatc agattttata aaataagtaa agttatctcc
     5041 acatgaaaca tgctttacat ctgatacatt aatctgcaca aattcatcca cttgatttat
     5101 agacaagtct tgcaataatt ctccatggta gtttgtacca catccataca ttgttccatc
     5161 attaagtaat ataataatat gagtcttaga aatagatact tcttttatat cttcatatgg
     5221 aagctcagta taaaggtctc ctttgtataa atttcctaat actaaaccat aatctatgtc
     5281 ttttgtggta ggtatcacta tcttattgtt tagaagtata aagttatgtc tgtttttaat
     5341 agatgtcaaa tcacccatta caaaattcat atgcttataa ttaaatattc tattatcatt
     5401 atttctacta tcgtatctat ttccaacacc taattgtcca tagttattta atccacaaat
     5461 atatatatca ttagtatctt ttattgctat atttgcatat tctaaaggaa gtattttctt
     5521 tactcttgta ttggttttta taaatgtatc tctttttgtt acatcaccaa cacctaattg
     5581 tccattatta ttaaaacctg taccatatag gtctccatta tcacttacat aatgtccaca
     5641 tctataattc attccaatat cctttgcctt tgatgctatt tttttaatct tctctggagt
     5701 ctcacttcct gtacctgaac cattactaca tttgcctgca gtatatacat ccccttcact
     5761 tgtaagtaaa agtactgcat tatttctgtt tggagctact tttactacat ttgaaattgg
     5821 aatttcagaa aatgaacttt gattgctatt actatttaga cctaagcaat aatcagtatt
     5881 taaacctgta ctatatgcag tattatagat tgttattata taggtattta tttcatttgg
     5941 tataacttct tttacatttt ccatacactt tataaaacta tactggtcag tagtatttcc
     6001 catacctagt tgtgaattat tattgtatcc acaacaatat gcaatgtttg tgtcctttac
     6061 aataaatgtt gttgtatctg agcagtacac atctcttaca ttagttatat tgtttacagt
     6121 aaatgtcaat acgtctggat ggttgctttc gcttcctaag cccagttgcc catgagtgtt
     6181 ttctccacat gaatataatg tgttatcatc tttgattgca tgcgagtgag tagtccctaa
     6241 aacaacatct cttacatttt ggatgtttac tttggtaaat gtatttctat ttactatatc
     6301 acctaatccc agttgtcctt tggtatttaa tcctgtggag tatagtgttc catcatttct
     6361 aattgcaaat gttgtgttac catctatact tgttgatatt ttctttatat tatcaaggtt
     6421 tatctgagtg aacttatttc tatttattgt gtcacctaat cctaattgtc cagaactatt
     6481 atttcctgtg gcataggctg ttccatcatt ttttactata aacattgtat ttcttgatgt
     6541 tgtaatttct tttatatcat ccataaatat aaatgagttt gattgttcag ttatatttgt
     6601 gaattttttt actggttcgt tgttatattc ccctatccca agtttgttat tctgattcat
     6661 tcctgttaca tatatattgt tatttttatc tactactact acagtatttc cttttacaat
     6721 tatatctttt atgtctttta atcctgtatc atagttactt gtgccatcta tatttgttga
     6781 attcccaaat tgcccactat tagcaatccc ttttccatat aacttggaat tactatgata
     6841 tattataaaa ttacttccta aattagaact ctcagaatta atactagtat aacttctatg
     6901 tccagtaatg gttgagccaa atgtttttgg atatccagta atatatgaac ttcctttctt
     6961 aaaaaataat atattatttg acatccctat tactttttca attccactaa aatcagtctc
     7021 ttgtataaaa taagtattat gatttgaatt tgtagcctca ataaaagttc catatcttga
     7081 ataatcagat atacttggac tgctatttaa tgttcttaaa tatccatgat atcctgttaa
     7141 ataaacataa cctccttcgt caattattgc acaaccatca taaaaaggaa aaacattagt
     7201 tattttaggt aacgttctaa gtccatttga tgtagaagat gttgaatttg ttctacattg
     7261 agatactaat gctttgcttt ggttactatt atttccttta cctaattgat aattataatt
     7321 gtatccagaa acatatagtt tcccttcact gtttatcata tatacagaat aatcatatgc
     7381 tgctatcttt tcaatattac ttattccctg cttatatgga taattagaag ttttttctat
     7441 attattataa ggattatttc cccatccata tacttctttt gagtgattta aagctatgac
     7501 ataattgctt cctacacaaa aatcctttac attctctatt tctatttttg taaatatatt
     7561 tctattaact tcatctctta aacccaattg tccattacta tttaaaccac atgaatataa
     7621 tgttccatta ttcttaagca caaaagttga gtttccgttt ccttttatat ccaaaacatt
     7681 atctatatcc accttagtca taactgacct gttaacagta tctcccaatc ctaactgtcc
     7741 ataattattt aaaccacaag catacaatgt accatcattc ataacaataa acatattgct
     7801 atctgttaac tctatcttct tataatcttg aaaattatca cttaacttta catttaaaaa
     7861 ttcactattg tcctttgtaa catataaata atcatcatta accaataaac ctgaataatt
     7921 atctaaattt gaagattgca catttgaaaa ctcatacaga aaagttgtat gaaaaaatga
     7981 accaagttga cctgctaaat tacagccaga tacaaatatc tcttttgaac catttattaa
     8041 aaaattaaat tgagaaccac atttaatatc ttttatattt tctacattaa ccttagcaaa
     8101 agtatttctt gatgctacat ctttattggc attggctagt tgaccataat tgttttgtcc
     8161 tgtactaaac agtgtattat cgtattttaa tatcatagta tgattatttc cacaagaagc
     8221 atacttaaca ttatttatac tagaaagagt aaatacaatt gaattattat tatttcctgt
     8281 accaagctga ccaactccat tatagcctgt agtatacata gttccatcat tcttaataag
     8341 aattgtatga ctactaccac acgctatctg ttttacatta tccatacctt ctattttatt
     8401 aaatacactt ttatttaaag tactattaat tcccaactgc ccatttgaat ttaatccaca
     8461 tgcatgtgct gtcccatcca tttttatgat aaatacagat tgattataag tcactacatc
     8521 tttcacacta tctatattta ctttagtaaa agtagctcta ttgttagtat cacctagtcc
     8581 aagttgacca cttgagttta aacctgctga atataatgtt ccatcatttt taagcacaaa
     8641 tgtaaagtca taaccacatg caacagtttt tacatcatct attggtagct tagtaaattc
     8701 ataatatact gtagactcaa ttacacctaa ttgacctaca ttacttgttc ctgctccata
     8761 tgcaaatcca tctttagtta ctgctacact gtgttttgaa ccacaagata catattttac
     8821 attatcaatt tttactttac taaacatcct tctctctgac gaatcttttc caagtcctag
     8881 ttgaaaacct gtattatcgc cacaagcata taaattacca tctgatttta aaccaaaggt
     8941 actattactt ataccagtaa aaaattctac aaaatcataa tcattatatc ttttatcaaa
     9001 atcatcaatc aatatgttct tatcattaaa ataagcacca cgctgtaaaa gtttattttg
     9061 cttcattatt agtactacct cctttattta tcttatttca tatgaatatt tacttagagt
     9121 tatatctttt tgttctttta aataagtgtt actagatttt gtctcaacaa agttattatc
     9181 catatcatgt atatatctaa ctctatttgc actaaatggt aatactacat ctctaaaaat
     9241 cacattacca atttcacctg ttatacattt tctatttaaa tctaacttaa gcccttctat
     9301 agctactatg ttatttccat ttgctaatgt atcataccaa taaccagcct catctacagt
     9361 tacttctgac tctaattcta ggtatattaa tatatctagc actttttcag ataatttgtt
     9421 atagctattt tttaatactg atatatcagt ttgtatttga ccacttataa catcatcaaa
     9481 agatatattt tctatttttt taactacctc atttatagct cctgataaat ctttagaatc
     9541 tgtagttaat atatctgtat ctccaatttt tacttttatt tcttttatat ttttatcaaa
     9601 cttatcatct aaatcttgaa tatccttttg agttacaaaa accaacgtcg ggtctacttc
     9661 taatgtaaca tttgaagtat tggacaattg taaaatcatt tttacaacca aatctttaat
     9721 actgccatct tcagcacgtg gcttatacgt ctctggatac ttacctatag ctattatatt
     9781 attctcatta tccagaacag cagcttctct tatcataaat ccaccaactg ttcctggtaa
     9841 aatagtctct ataactatcc aattaggatt tttttcatca attcttagag aatttatatt
     9901 tccttcccaa actacattct ttaaagcagt ttgttcctct gttggctcat aatattctcc
     9961 attactatct ccaacttgaa acttggttaa tccaacaagc tctcctagtg cagttgcatt
    10021 tgctattttt tctctaccaa ttttggttaa tattatataa aatttttctt ccaattatac
    10081 cacctccgat ttaggataaa ctactacatt ttctacgcct gtatcatttg caatagctat
    10141 attaatctta caattatttt caatgcattt aatgcttaaa ggataaacct ttatttcttc
    10201 tctacttaac attccagctc cacagtttac aacagcacta cattttataa ttggttcaaa
    10261 gtatggatga acttttacct cttcactaca taccatacct ccaccacaat agaccatact
    10321 tttatctagt attattggtt ctaaatagtg taataagtga gcaggtttta ctctttcaat
    10381 catctcgcta caatctaaaa gagttttata gtcacagtta tttgctataa aactcaatac
    10441 gaatgtaaat tcatcactat aaacttttat atctgtttct gattttgtat atgcctcaca
    10501 tatacttttt ataacttcaa tagtactagt acctctgctt ctcattttag cttttatatt
    10561 gctacgtctt gtgtcaaaat caagttcttt tttttcaatg cagagtatgt cttcccacat
    10621 atctaatccc catgtcgctg tatcaacaaa taattggtta aaggtatcat caatatcttc
    10681 tttaagaata tttaattctg tatcgtatgc accttgtatc tcctctacaa tgtaatttct
    10741 atcaaatgat ggtagtttat caattagctt cattgatttt ccacctcact aaaattaaca
    10801 gttgttacac taggtatttt atcttcatta atagttatat tatctgtact tccatttata
    10861 agtagattac ttaaatcgtg tacaccagta gtatttataa gtattcccat gacttttgta
    10921 tagattatct ctcctctaat atctctaaag tatgtattta tactttctag gaaagattct
    10981 tttacattgt ctaatgtata tccatcttct agtttcatta ctgcacttat acttatttct
    11041 attggtaatg gtgtcacaac tgttatagtt ggtccaatag gcttctcttc atctatatgt
    11101 tgctgacacc tttcaatcgt ttctgtatca acagcttgat tattttcccc aaatatcaag
    11161 accttaactg ttcctggacc atcccatctt ggataaacct taacattata gactccatct
    11221 acttccaaag cccattcttc atagtgagct ttatttccac ttgtagcttg atttctttgg
    11281 attttataaa atctttcttt tagttcttca tctgtctcta tatctgtacc accttcaaaa
    11341 cttcttgtgt tagtaatctt tgttactcca gatatattat caactagttt aaattcacaa
    11401 tttgtaggta aattatattt cttaccaact tccagagctt gaactgggct attgtcacct
    11461 tcttcactac caatagttac atcttttatt actacaaata gtaaatctct atatgatatt
    11521 attgtgccat tatttattac agttcctttc tctccaataa attcaacttc tccatttgac
    11581 tctgtaccta actttctata tacaccaaat tcattgactc ttttatcaag aaactggtta
    11641 tatgtgtctt gtataaaagc cattttatgt atctttgaaa gttctagata tatcttcgaa
    11701 agttctagat tatttccaga taccatgttg tttagaaaag aaccttctcc tttataaata
    11761 tcaagattaa tattttcaag agttctattt tttattactt catatgtctg gtcactatac
    11821 atttatttcc acctccccat agatagtgga tacttttaca tttgcactca atacgtctct
    11881 gttaaagtta gcacttttta cgtttacttc taatatatat ggatttatta gaagggcctc
    11941 ttttatgaat ctactagctt cactttctgt aagacctttt gtatattttt gtcctattag
    12001 ttctgaaagc tctgtcccat attctaaaga gtatatctca tgttcatatc tatttgtctt
    12061 tatacacttg tatatccaaa cttttaaggc ttcttttttt tcaattattt taaagtcacc
    12121 tttttctaaa ataggttcat ctttttcaaa atcccatgcc acttcacgaa agattggcaa
    12181 ttcttctgtt ttaggtaaga tataatcctc tgggacacct ataaaaggaa atattgtact
    12241 cattataagc tcaccaactt acttacaaca gcaaattttt cgcctattct aaacattatt
    12301 accatatccc cagattcaaa gttatctata aaaggatttt ttacttcatg ctggtgttct
    12361 tgatttgttt ctgtgtcaaa tgtttctagc tgtctatcaa tcatccaact atctatcaaa
    12421 atatcttctt tttctaatat gatgttattt gtctctattt ttaaatctgg taatttactt
    12481 ttaatttttc caataaaaaa agaaggttga ttatgatact ttccttcttc tcttattatt
    12541 cctataaatt catttattgg attagccatt atatcaccat ccttttataa tactctccta
    12601 gctgtattaa aatctcttct actacttaac ttacttattt taactacatc acctttttgt
    12661 ggagcatgta aaaattctcc attacctata tataatccaa catggctgac tggattatga
    12721 aaaaatacta aatctcctgc ttgaagattt ttttgttcta ctttcttgcc ttttttagat
    12781 tgttgatttg acgttcttgg caaactaaca ttaacttttt taaaacagta gaccattaaa
    12841 ccagaacagt caaaactact tggtccattt ccaccccatt tataaggttt acctagatgt
    12901 ttttttgcct ctgatattac tattttagct ttatcagata cattaccaga ataaccacca
    12961 ataataattt ttccttttct tcttccaaat ttactagctt cttcaacatt tccaaataaa
    13021 atatctatat ggtatgttcc atttttttct atagttattg caggtcctac gtcatttact
    13081 ttatagactc catcacgacc agaaactcct gtaattacct gtatttcatc tccatagttc
    13141 aataaaggat gtttatttaa aaactctttt gtataataag tttgctcata tttaccaacc
    13201 ataggagcag cgcaagtttt tttagatggg tcaagttttt tctttctaca atctgtatct
    13261 ccaccttctt cttttctagg acagtaagct gtaaattctg ctgtaaactc ttttcctcct
    13321 gcataatctt ctccccctaa attactttct tccttaggtt catcctgtcc tgctgacttt
    13381 tcatccataa gattttgaaa attaagttca agctcaattt gatattctcc attttgccaa
    13441 gtatgtttgt ctgtatctat ataaaatagt cctacaagct ttgtataaga atctttaact
    13501 tttactcctc taccagttat acaacttaca tctccataac ctttaagaga acagcttttt
    13561 tctatcccat taaactcgct atcaatatct acatcttgat tttcttgttg ctgaattact
    13621 ttttgcatta ttacatttac ttccttaaaa atttcattat ctattttttc gctaatcttg
    13681 cttccatact ggtctaccac tattacttta ttttttacat tttccatgct ctccgaaaag
    13741 gtggtattta taatattaaa tccctcttca aacataacac ttaatgtaac agttcctttt
    13801 tcaataacat taaacttatc taaattagcc tctatcatat actttttctt tgtcttttta
    13861 cttgcctctg tatatgcact cattattgtg tcataaccat ttactcctat aaacatcttt
    13921 gtatacttga cattggtctt tgctattgtt ccaactgaaa gcctattttc agcaaatact
    13981 tgctttgcta tgtcttcaac taacttatct ttaaaattgt atgacacttc actttgtgta
    14041 agtaaaaatc ccatatcttt agatacaaaa ctaatttcat tactgcttga atctttagac
    14101 ctattaatta tcattcctct aaagagttct ttctcatcta catagaaaca gactgtacta
    14161 gctattggta tatcgatttg ttggaaattt acatcaaatg atgattgtat tattgaaaac
    14221 tctagtgtcc ttgatggaga tttataatca cctgaccatg ataccttgtc tactatgtca
    14281 gttatgtcat atatacttcc gttttttatg tgtacccaaa tcttaataat aaaaaatcac
    14341 ctgccttttc atttaattct atcagcagtt acttacaact tattatctat aatagttatt
    14401 gttttggagt tacttttggt atctttattc ttttatactc ttttaaatct aatgtaaaat
    14461 atacatctcg agtcccatct ctttcttcat aactaaaccc ttcaattatg acttccatat
    14521 ttatatttgt ttccgtaatt gtaaatctta atataaaacc ttccttcatc catttttcta
    14581 tcttatttac acaatcatat ggtgatggaa aacctgtata gtcacagaaa gtgtagtctt
    14641 tatttggaaa aaaacttgat attgaaatgc ttttaagagc tacaccacca aaagttgcta
    14701 cttcacctat tttgagtata ctagaagagt ttacagcagc ttttccatta atactaaagg
    14761 aagatggaaa tactggaaat ctaaatctat cttctgcttg tctaagccac atttccatta
    14821 taagacaccc cctaactgtg ccaattggat acttgcaact aattttgatg ttatcttttc
    14881 tatatcagct tcttctctaa tcactattgt atcagctaac ttagctatgt ttatatttcc
    14941 accatttttt ctgcttctat attgatttgc ttcttgttta gttaaaacca tttcaccttc
    15001 atggagtctg gtttgatagt cattataagg aacatagttc aatccaaatg cattattacc
    15061 tttcttactt gaataagctt taccagaatc accagccttt gttccagcat atggattatc
    15121 tttgtaattt tttttactct tttccactag atttaaaact gttcctgtga tgcttccatt
    15181 ttttatctta ttaatcatag tttgccacca tttgcatata tctttaacta ttttacatac
    15241 tttatctaat atgttaaaaa aagctcccaa tattggctct ataattttcc atccagtttc
    15301 caacagaggt cctaaggtct cccatacagt tttccaaata acacccagtg tctctataaa
    15361 attgtttatt tgaggtgcat attcacctaa aaaagtaaaa attcttgtga taataggact
    15421 aacagcttca actacaggtc cgattagttg gaatgctgta gttactattg taccaaatat
    15481 acttattaat ccttgtgcaa ttggtgattt attgtttata ctatctagta aattatttac
    15541 taagtttact actggaagta gtgaatctat taatgctgta aaatcaatat tttgtaatag
    15601 tgtacttcct gcgttaacaa ttgcttttgg tgcattccct atcattgttg ctgcatgatt
    15661 ttcaaaacgt tcttgcttat tttcaaccat cattttacgc ttttcagcct cattagaaat
    15721 tcgatattga gtagaatcag aaaactcttt tccatttttt acacttctag attgcatctg
    15781 gtcatatttg ttgccaataa ttatcttacc attcattaca ttgcctattt taccagccat
    15841 tttttcagat aagccagcta aattccctaa tccactttct ataaaagttt ttattttctt
    15901 tagtccttga cctaaaatat catcaaaagg tttaacaata ctcttcaaac cattgttaat
    15961 gcttgtttga gctcctgata ttatacctgg tattgtatta ctcatttctt tagctaaccc
    16021 catctgcttt acacttatgc cttccaatgc ttcatcaaaa gttttataat tttctcctac
    16081 cagagtatta agagattcca tatttccttt tgctgcactt aagaaagctg ctgatacatc
    16141 ttgttcacta cttgtattca tatttaaagc tctaacatct accatagctt gagttatatc
    16201 tttttctttt ttgccaccag taatattact aatcatttta gctttagttc ccattgcaac
    16261 aacatcttca gggtcaaaag gcgtatcatt tgccatattt actaaactgt cataatactt
    16321 atcaacatct ttttggctag tttttccagt atttcttttc attacacttt ctagaacaac
    16381 tttctgattt tgttcctgac caagtaagtc aacacccttt ttcacagcga cacctatagt
    16441 agcaccattt gtcaaatcag atatcttccc ttttatcgaa ccaagtattc cacttgccat
    16501 atcctttaca gctattgcac cactccatac cttacctgca aacaatctca atttgggaaa
    16561 tactttactt ataatgctac ttgccatatc ttttatagct attacaccac tccaaaaagt
    16621 atttttaaac aacattaact taggcaaaat tctagccata gcactactta ccatatcttt
    16681 aagattaata cttccttgat aaggacgttt ctctactcgt tttaattttc ccaagatact
    16741 acttatcact ctcttagtca tatcttttgc ttttattacg atattccaaa tcttgccacc
    16801 aatctttttt aaatttccaa gtaatttatt tttagttttt tctacttcat cacttttagc
    16861 tttaaatatt aatataatca taatatttga attaaactca tttttctttt gaatcaactt
    16921 attaaaataa tcaataatca tagctatagt tattgctagt ctcataatac ttccatttaa
    16981 acttccaata actcctgaaa cagcatttat gctagaactt aattttgcca tagttgaact
    17041 tacactacta gcagtagttt gaacttttct taacgaggaa tttaaactgt ttactctact
    17101 acttgcactt ccaatagaat tttttatgct actagccatt acattagtgg aaagtgaaac
    17161 agccacagtt gctctcctaa aattatcagt tactgatata atttggttca tttttggtgt
    17221 gtaattatct gtaagataca tatttatacc ttcttctctc acatttccca ttacatacca
    17281 cctcctgtca aaggacacac tccatatcca tgtttcattt tttcaacttc ttcctcaatt
    17341 tctaaggcga aaaaagcttc aataagcttt aattcgcctt tatccattgc ataaaaaagg
    17401 gacggtctta tacctttttt cttccaataa tagtacatta ttgtagtctt ggtatccgtc
    17461 cctattagtt ttttacctct tctatcactg catttttacc ataacccata acatcttgaa
    17521 gtattctata taggttggta atttcacctg gtaataatag ttttcttgcc aattcttttg
    17581 gtgttggagc cttaaattta tccattagtt ccttattttt aaataaaagc tttccatcca
    17641 aatcacaaac accttctata attgtaagca tctgcatctc cggtaagtct atatccgttt
    17701 tcatattttc atcaatttta agacaagtat tttgtatctc ttcatcttta cttattgtta
    17761 aagctctaca cattacttta aattcctgcc caaatatagt tgataatctc ttaagttcaa
    17821 ctatagtact tggtctttct aagttttctg tatctgcatt taaaagtaaa tctactatgt
    17881 ttatattttt tgataatcca ttttcattca ttattatttg ctcctttatc aatcaaattc
    17941 aatcaactaa cttttttact tattaactac ctaaatttat taagtatata tctactaaat
    18001 atcagtataa aaccaaatat aaaactaaat tatatcaaga aaatcatact cagtaaatgt
    18061 gaaaggtgct tctatctctc cttttactcc aacctcccaa tcagctaaag ttaaatcatc
    18121 aaatgctata ttttttactg ctattctttc tgctccataa gaatctgggt catttaattt
    18181 agatatagca acaaatctag gttcagaacc tctctttatc ttttctccaa ttaacttgtg
    18241 cattcttgaa ctaacatggt atagagttat tgaacctttt cctttatatc ccatatactt
    18301 tgtatcagta cccatttgac ctgctattat aatatcctct tttgtaaatt ccatctttgc
    18361 ttgaaacttc tttacttctg ctactttgtt tccatcaagc caaagttctc cccaagtacc
    18421 actcattaca tttctagctt ccatatttgc catattaata atcctcctat atttctattg
    18481 ataaatctat atcttccata gcatcaagta cttttatttt tgcttttaaa aatactttag
    18541 aacctgtgtt agcttctttt atttcttgta atgtcatata agataaatct actccttttg
    18601 attttaaata cgatttttgt gcttcaaaat ctatttcaac agtagaatca gattctataa
    18661 gtgctgactt ttctaattct tctaaataac tttttatagc tactatcaat aaacatttgt
    18721 tgtcataact gttagtaacc tttcctatat agtcatctat tatcaccttt cttatgtcac
    18781 tatgtataat atctaaagtg tcaactattt ttattttctg gaacatttct cctttttctt
    18841 ctgttaactc agttaaagaa tttactcctc ttgcaattct tatagcccct gcttccttaa
    18901 taagtataag ctctccttta ttaacccttg attctgcatc aactttcgtc atcttaggta
    18961 tatcaactac atcactaagc ttagtataag ttactgattg acttaaaggt gttcctgcta
    19021 taagtccagc caccctactt gtaaactcat caacactgta tttcttttct ccaactaaca
    19081 catcttctgt agtaaaatta attatccctt catgatttcc tacaactttt cctaatacag
    19141 ctttaacctt aaccttatct atatctctaa gtttaattat ccaatttttt atagcagtct
    19201 tatcagcttc tactgctttt ggcatacata gataattaaa ttccttagtc tctaaaaaat
    19261 ctaatgcagt ttgaatatct gcttctcctt ctattacata aactaataat ttatttggag
    19321 tgttaacatt tcccatcaaa gctaaattta tatactcttt attttcagca gataaatcaa
    19381 ctggtatatc ctctttttca tgtatttcat taagacctag tgccttagca tctttaagca
    19441 ccattgcaat tattcctcta gctgaacgtt ctttaacagt tgtagctagc tccttaaatg
    19501 atatgttgat acttggtaat cctatagcca tactatcact ctcctaaatt taaattgatt
    19561 tcttccatat tttcatatac tacatcttct tcatatacac tatccagata atttagtgta
    19621 attgtaaagt tcaatacata aattgactct tcttttttaa tactttgttc tagctttgtt
    19681 atatttaatt ttctgtctgt tacctttata tttctgttga ataacttatt taattcatct
    19741 gaaatttcat acattctaat actctttgac tgtggttttg gtaagtattt tatagagata
    19801 acaatatctt tattattcgt atttaacatt ataggtgtac aaataactga tagtatactt
    19861 acaaaaaaac aagacccttc agagccttgc acactttcat catcaataaa aatactttca
    19921 gtaaagtttt ttttgagaat ctcaataatt gtttctaata tttccttgta tttaagcaaa
    19981 taatcaccac actatcacta aatattcttg cttaatctga taatcttact ataaatgata
    20041 taagtcattt ttaaatatac atctcattta ttcctgtcaa atatcttcta tatttttagg
    20101 taatattatt cttttcaatt tatcacctcc ttcaatatgg atttctttca caataacatt
    20161 atctcacata ttttattgcc tagtgttgca atagtgtata taaagtgttg taaagttgtc
    20221 atatttttta taatatctca ctcaatttaa atatcatttt tttctttaaa ttgtagcaag
    20281 atgttctatc taagtgtagt ttcatagaaa tatatgtcat attgtttttt gtcttactat
    20341 tataaaaaag attaaaaaat tccatttcta tatcatttag acatgttaga gcattttcta
    20401 tcttctcttt ttctatttct atatcttctt tttccatttt taattttctt aatctttcct
    20461 ctctttttaa cacctcattt tccacagaag aacttatgtt atatgtgtta ctagtctttt
    20521 ctgtatatac cattgctcca catccagagt attcattctc acatcttttt atgtccaaat
    20581 taatattctt tatctttata tccaaatact tataattatg taatctttca tctgtttctt
    20641 taaataatat atcttttttg ttttccatag tacttctcct tttttatata cttattatta
    20701 gcttcaaatt tacaataatc tctttagtgc acagtttttt actaatctca caactttcaa
    20761 gaacatattg ttctataact gtataaaaaa aacttcttct atattttgtt ctaaaatact
    20821 agaaatcttc ttagccataa gaagaccagg attttttata cctctttcta tttgagatat
    20881 atagtcattt gtaattccag cctttctggc aagttgaatt tgtgtcatac ctttgcattt
    20941 tctataatat ttcaagttat ttttaaacat tgttttatac ctccattacc attaagaata
    21001 ttttgttctt ccacacttag attatataga atcatttgtt ctttatcaat agattttaga
    21061 aaaaaatatt cttttatatt ttattagttt tattttttag aacaatttgt tataatctat
    21121 acataaggtg gtgttataat gtttgccaaa agattaagag aacttagaaa ggaatttgga
    21181 ttgactcaaa gagaacttgg agaaaaagta ggcgtttccc aaagagtctt agggtactat
    21241 gaaacagaaa acagatttcc tgatgaacac attttaaaca aattggctga tgtatttaat
    21301 gtatcagttg attaccttct tggaagaacc ttagttaagg aaaatattga tacagtagct
    21361 gcacatagaa aaaatccaca tgaagagtta cctgaagaag cacaagaaca acttaatgat
    21421 tatatagaat ttttactaaa taaatataaa aaaaaataaa ccatatgatg agcagtctat
    21481 ttttctgctc ttttaatata atttaaaggc aaacatacat tctattttag ggggattttt
    21541 atgaataatt tggataagct ttttgaatta gcttctcaag aagagataat aattcattat
    21601 accacttata ttgcaggtga tttagaaggg ctatatataa acaaacatgg tattaaaatt
    21661 atatcattac tcagtaattt aaaacaaaac tcaaaaaaat tgacatccat cttagctgaa
    21721 gaacttggac atcatttcac tagccttgga tactatgtat cctcttataa cgattattat
    21781 acaaaaatta ttatagataa gtgcgaaaat aaagccttaa aatgggcttg tgagttctta
    21841 attactgaag aagatataat aaacataatt aattctggca tcacttgcgt ttatgaaatg
    21901 gcagacatac ttaatgtgga tattactttc tttcaaaaaa gattagaatt tttatcatta
    21961 aaaaaacagt ctttacaact tggaaataat aaatatttaa tattaactaa tttgccgtat
    22021 ttctacatat ttgatccaat ttcataataa aaatagtagt aattttaact ggcttaacag
    22081 taaaatctat atatttattt taacaaattt tggtttctat aaattcaata ttaatatata
    22141 ataatgatat catattattt aaaattctaa aaacaaatat atgcttttgt taagaaaggt
    22201 tgtttgatga atgtataaag aaaagttgtt tgataattat tttaagttct tagcactatt
    22261 attttggcca attatgtggt ataagtggat agtaatttca aatggtactc ttgaaaatat
    22321 gttatttacc atatatgcaa tcgtcgcaat tattttcatc attttatatt cggtgtttat
    22381 gataaaatat aaagacatta cacaaataga tttcttttac aggataagta ctctacttgc
    22441 ttttatattt acacttttta gctttttaat atacccaaag agtttatttt tcttgtattt
    22501 aaagattatt tttactggaa tataccttta ttattcaata gtaaaaactc ttaaatttaa
    22561 agatgatgaa ggtgtagtgg gtattatgag ctcactttta ttaattgtaa taactttgtt
    22621 ctactaaatt taattctatt tgacttaata tggctttaaa tattagacat ctacattatt
    22681 ttctgatatt atttaagatg gtagtcttat attaataatg aaatacacta tgaaggagtt
    22741 aactattatg gaaaaaaaag ttttagcaac agttggagaa aaagagataa ctaatattga
    22801 tattgaaaat gcattaaaat cattagaccc ttatcaagct atgcaattta aaactgaaga
    22861 aggaaaaaaa cacttattaa atgatttagt gaatcaagaa ttattcttct tagatgctaa
    22921 agaagaaaaa ttagatgaag aagaaatatt taaattagaa atgaaaaaga tagaagaaaa
    22981 tgtattaaag caatttgcta taaataaagt tttatctagt gttaatgtaa cagaagatga
    23041 aaaagtaaaa ttctttgaag ctaataagag ctctttctct aaaccagaaa gtgcaactgc
    23101 taaacatata ttagtagata gtgatgaaaa agctaaagaa atacttgctc aaataaaatc
    23161 agaagaaata tcttttgaag atgcagctct taaacattct tcttgcccat ctaaagatat
    23221 gggtggtgat ttaggaacat tcggtagagg acaaatggtt cctgaatttg aagaagctgt
    23281 gttctctatg gctaaaggtg aagtaagtga accagttaaa actcaatttg gataccatat
    23341 aataaaatta gaagaccttc aagaaagtac agagtctaca tttgatgaag ttaaggctga
    23401 agttgaaaaa agtttattat atcaaaaaca aaatgaagtt tatggaaata agataaatgc
    23461 tcttaatgct aagtacggaa accttgtaaa atacaacgat taatcataag gtcatatata
    23521 aaaaagctgt cttttcacaa tatttattag aaaagacagc tttttatatt aaagtttaaa
    23581 tattcagttt catattatca ttcttttatt gtaaagaact cagcttaaaa gtttaaataa
    23641 gatttttaat tatcattagc taaattttta tatccacgta ttatctcata acagtaacct
    23701 attacatctc ttctttttat tatcccaata aatgtgtctt ggtcatctat aacaggaatg
    23761 aaattttgat ttaaagattt tataactaaa tcttcaatat ctgcgttgat ggaaacagga
    23821 gaattatcca tttttctatt tatatctgtc actggaacat cttccaatcc ctttaagtcc
    23881 agatttaaat cgttttttaa tgtccataaa aaatcacctt ctgttatagt tccaacatat
    23941 ttaccatcct tacttattat tggaattgca gagtatttgt gatattccat tttttctagt
    24001 gcttgcctta tagtgtagtc ttcatatata tatgctactt cactttttgg tgttagaaag
    24061 aataatatgt tcattacact acccaccgtt actgaaaact tataaatatt actcaatatt
    24121 tataatgtta cttttttgca gtaactttct cctttcttat tttctaagta ttcttaatac
    24181 tatacgctgt aacaagcgtt tttcctttgc tcattttaat cttattatgc tttattaata
    24241 tttttatatg gtctatcagt atcacttatt ctctttttag aaaatttact aaaaattaat
    24301 tgacttaaac ttagccataa taattttact ctacactatt aattttaaca tatttatctc
    24361 attattaatg attttatttt aataatttta atttattttg ctattaattt tatttacaaa
    24421 aactgaaaaa accgctaaat gattagcggc ttatctctca tattactaga acactaatat
    24481 atatgaattt ttaattataa tctaactacg ttagcagctt gaggaccttt agctccatct
    24541 actacttcga actcaacgct ttgtccttct tctaatgttt tgtatccatc tccagttata
    24601 gctgagaaat gtacgaaaac atctccttca ccttctacag atatgaatcc aaatcctttt
    24661 tcgttgttaa accatttaac tacaccagtt ttcattaata aatcctccta aaaaaacaca
    24721 aatatttagc tactcttttc tgtaacctta ttactactct atcatatata gctatgtttg
    24781 tcaattatta ttatttttat acattaataa tcaatttttt tactttataa ttttaaatga
    24841 ttattatctt ttacacttta tttttcttta taatttttta ataatttatt tttattatat
    24901 ggaatcttat ggacttatca ctccagccta aatcccatgg caccaacatt ctatcagtat
    24961 tatggcaagt taccaaagga tatcctcttg agtctaatcc tgtcactgtc gaaacatgtg
    25021 ttattctgcc tttcttctca taaccaacaa aatcccccgg acgtaaatta taagcttctt
    25081 tgtatacttc ttcataggaa cctttagcaa ttaatgaacc tcttccacta tacagtgcat
    25141 aatttttaaa agcttgtgca tttacccatg cttttgtacc tgctctctcg ctataattcc
    25201 aaattgagtt ctttttgaac cttccacttt caaacattat ctgtgatgca aagtttgcac
    25261 aatctccacc ttctggatta taatccctat agtttggatt aaatttcata ccatgttctt
    25321 catcagctgc ggctccacag tatttatgtg cataatctat agcctttttt tgttcatctg
    25381 taagctgtaa ctctacacca tcttgttgcc caatatagtt tcttatatcc tcagatttta
    25441 tattttctaa atttaaagag tctgcaaaag ggtctgtgta ccactcttta gttatgacat
    25501 attgattatc tataattttt acatttagat aatgatatgt acctattcta aaggcatttt
    25561 ctttgtcctt ttgattttca tatgaatact tatattcagt cgatactgaa cagattatac
    25621 catagaggtc tttttctttt tcttttattt ttttaatctt aacccttgtc tttatgtctt
    25681 taaattttac accttgctta ctagaccagt tttctaaata cttcatcttt tttacttcat
    25741 tttcataagc ccacagacca aattttttat ccgtctcata aagacctttt aaaatgtttt
    25801 catttttctc taatattgcc ttatttctgt agtcaaataa gttttcaagc aatagtttat
    25861 attggtcttt tacatcatca ccttcaacat ttgcagattc catgactttt tcttccatat
    25921 ttttcatact taaatcattg attaaaaata cacctgctgt agtagctaaa gacaaaaata
    25981 caaatgccat tatacctttg atgtacttat tcttattttt cagtattttc aaagctacac
    26041 ctcccaaata gtacacctta taaatgaaat tacaaagaag attctgtcat atacaataat
    26101 tttcatattt aataattctc tattttatta tgtccaatat taaaaattaa ttgtatcaat
    26161 acactgtttt ttttacaatt ttttatttta taatctactt ttttcttttt tttatagtaa
    26221 tatatatgta tataaatatt ttggtatcat tataattaat acatactaat ggagggtttt
    26281 ttatgcaaaa aaaagtcgca gcaattaatg acttatctgg aataggaaaa tgctcattat
    26341 cggttgctat acctatactt tcagcattaa aggttcaatg ttgtccattt ccaacggcca
    26401 tactttcaag tcaaactggt tatcctgaat ttactttctt agactttact gatgaaatgg
    26461 taaaatattc aaatgtttgg aaaaatttaa aagtcaattt tgatagtata tatagtggtt
    26521 ttttgggttc aaagcatcaa attgagatag tggctaattt tataaatgat tatcccaatg
    26581 cttttatcgt agttgaccct gtgatgggtg ataatggagt tatgtatcca atattcacag
    26641 aagaaatgag acaggaaatt aaagaattag ttaaacattc agatttaact actcctaatt
    26701 taacagaagc ttgtttctta actggaaatg attatactaa aagcgattat aatagagatg
    26761 agcttatata tattgcaaaa tctgtatctg atttgggacc tagcaaagtc gttattactg
    26821 gtatattaga agatgataat atattaaatt tggcttatga tagagataat gaccatgtat
    26881 tctttacatc agtcaaatac aataattgtt cttacagtgg aactggagat atattcacat
    26941 ctatactttg tggtatgtta gtaaataaac atgatttggg agtagcggtt aatactgcaa
    27001 cagattttat atataaaact ataaattaca catctcaatt tgatactgat agaaatgatg
    27061 gagttatgtt tgaaaacttt ttatcagatt taactaatat ataaaatcaa aaaaactatc
    27121 cattttataa atacctataa atcataaaat ttaggtattt acaaaatgga tattatttac
    27181 taaactattg gtatttcaat tgttaccata gcaccaccag ttttcacaga attttcaata
    27241 tataaaattc cattatgttt ttgtacaata ttatttacaa tataaagtcc cattccgtaa
    27301 tgagatttag aatttctact caaatctcct gtataaaact gttctgtagc ttttgacaaa
    27361 tctgctttag aaaaaccact tccacaatct gtaactacaa attttaagta tttattactt
    27421 actgatacac ttatgtatag tttactctgg cttggtgaat aatctatagc atttgaaatt
    27481 atattcataa tagctctgaa taacagttct ctatctatag ttatattttt aggcaaatta
    27541 tcttgtttat aaaactccac atctaagttt ttagtactag ctaatgcttc tatttgcagt
    27601 aagacatctt ttataaactc actcatatta attgcttctt tatttaatat atagccattc
    27661 tctgtttttg atatatctat taaagtatta atatattttt ctatttgagt gacacccttt
    27721 gagatatatt ccatgtattc taataactca ggattttcat ttatttcaat taataagtca
    27781 gtatttccat gtataattgt taatggtgtt tttatatcat gtgccagtgc agatatctgt
    27841 tcctttctat tttcttctag catccattgt ttttctaaag aattctttaa cgccaacttc
    27901 attttatcca tagaaaaaag gatattgtta atttcataaa ttttactaga ttctacaaca
    27961 aaatctaaat cctgttgttc tattttttct gtagtatttt ttaaaagctc catttcaata
    28021 ttaaatttct tgccaaatac ttttgataat ataacaattt caataaaaaa tattatacta
    28081 aaaataatca tacctaacat ctctggtttt ggtaaatatg ctcttaaaac tggagaacta
    28141 tactctgata ctagtttgta ttttattata aaaacttcat ctttcttaaa aaactttata
    28201 taactttctg aatatgcatg cctttcttca atatctctca ttaaattcca tacttgtttt
    28261 gattcttttt tattaaaagt gcctgagatc aaattcccat ctaaagtata aacaccatac
    28321 tcgtataaat ttggaattat atcttcaatt gctttttcac ttgaaataat cttttcttta
    28381 tatttatcaa attgtttttc tgaataatta gctggtaata ttacacctga tgataataat
    28441 acagaaaatg ataacattaa aagaagtgca aatgaaattg tcataacaaa gaataaagat
    28501 aaatatttta taaaaatagt tcttaacttt tcaacttttt tgttgttaga ttttttactt
    28561 ctaagcattt gtgccatatc ctccaaatat tttaattaat tcataattaa tacctaacca
    28621 gtttttattt gattatttta tatttttcat tgctaagtta tattttaaca taataatata
    28681 aatataatgt aaataaacag tcaattttta tttattcttc tgacttttta ccttcccatt
    28741 tagaaaacca aatacataaa attacaaatg ctaaaattgt tacaaaacca cacattaaac
    28801 ctgttgattg atatgctttt attgattcaa cctttatata ttcaaccgtt ttagaacttg
    28861 aaaaagacga ccaaatagtt ataaatctaa caccccatgc acaaggaaaa tactgccaaa
    28921 tgccagaacc caatccagtt aataataatg ctgaaagtaa catttcaact atacctactc
    28981 caattgatgt tccttttccg aaccttaaac ttaagaataa atgtaatatg tattcaaata
    29041 aattacaccc aattaaaatt agaattgaaa tcatataaaa gcttgatgta aagtaagcct
    29101 tagatatacc aaatttaaaa cctaatacag acactaatgt tgctacaaaa ccaagacata
    29161 ctaagattat atatttactt ataagagtaa gatattttgg attagatgtt gttaacatat
    29221 gtttataatt ccctgcataa tattcttgtt caacaaacat tgaacacaca acacctgaca
    29281 ataaaggaaa agctattgct ataacttgta aatatgctac tatctttaat atattatcag
    29341 ctcctgaaac cttataatac cataaaaaca atcccatccc cattaaagca atgcaaatgt
    29401 gcattagtat tataaaattt ctctttaatt ttattaaatc agaacaaaat attcttatta
    29461 gttgtctcat attacttcac ctctctatta ttgaaccaag ctcttgtaac taaagcaaat
    29521 aatacaaata aaataacaga tattaatact cctggtaaaa taacagcatt atttaaaagt
    29581 tcactcccct ctggtacagg aagcccattt ggtagaatat gcaatacaac aaccattaat
    29641 cttgaaggaa tcgaatatgg aaatacatac cacatttctg tagttgagaa aacagcaact
    29701 ccaacaatag taaagaacat atttgttaga actgtaacaa atataccaaa ttttgaagct
    29761 aaaaacatac aaagaggaat ctgccacata aatagtaaaa taattaatat agttgcaatg
    29821 atattttgac ctattgaaat agtttcttta tagattatcc caacaaaaac tacacctatt
    29881 gcagaaataa tcgatgctat agaacaacaa ataatacagt aaatcatttt tccagcccaa
    29941 acgtctttca tttcaattgg caatggtaaa ataccacgat atttactttt ttcatctttt
    30001 aatactatta aagaagatat taatgttaat gctcctggaa gaaatgtcgt ataccaccag
    30061 ttgaaagcag cattttgccc tccacctagt ccacacaaaa gtatacaaaa cactggaact
    30121 atccatatca acttcattcc aaaagttcct ttcatctttt gaaattccac tctaataaat
    30181 ctttccatat tactcccctc tctttctatt ttcagctaca actttcataa aaagtttttc
    30241 taagttttgt tcttttgata cttctccttg ataaccaatt actccattag aaattattcc
    30301 gatttcatct acgacttgtt caacttcaga caagatatgg cttgataaaa taactgtaat
    30361 tccttgtttt ggaaaagaac gaatcaattt tctaagttct tcaataccaa tagggtcaag
    30421 accatttgta ggctcgtcta agattaaaag ttttgggtga tttaataatg caatagcaat
    30481 accaagacgt tgcttcatac ctagagagaa ttgacctgaa cgctttttaa aagtatctct
    30541 taagtcgaca gtctctaaaa cttcttcaat tcgagagtca ggaagaccaa gtaaagtagt
    30601 atggactttt aaattttctc gtgctgttaa attctcatat atagcaggag actcaataag
    30661 agaaccaata tttgataaat cctttctagt ccatctatgt ccctcaaaca ttatttttcc
    30721 tgaagttgga tttaaaatcc cagttatcat ttttaataaa gtagatttcc cagctccatt
    30781 tggtcctaat aatccatata tagaattaga tttaattgct agtgaaatat tatcaactgc
    30841 catctgctcc ttaaattttt tagaaactcc ttttacctgc aaaatataat tattcataat
    30901 tttcatcctt tcttttgtca aacttattta caagaatcat tctaaatcat aaatataagg
    30961 attttataag aattaaaaaa caaaaaaaag atgtgttaaa aacacatctt tttttatctt
    31021 a
//
LOCUS       DAGQWR010000034        30682 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.034, whole genome shotgun sequence.
ACCESSION   DAGQWR010000034 DAGQWR010000000
VERSION     DAGQWR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.034"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            137..2038
                     /locus_tag="MIW95_001334"
     CDS             137..2038
                     /locus_tag="MIW95_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY3150676.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASNLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2041..3084
                     /gene="ypdE"
                     /locus_tag="MIW95_001335"
     CDS             2041..3084
                     /gene="ypdE"
                     /locus_tag="MIW95_001335"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBY3150677.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3099..3404
                     /locus_tag="MIW95_001336"
     CDS             3099..3404
                     /locus_tag="MIW95_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY3150678.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPTMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3501..3836
                     /locus_tag="MIW95_001337"
     CDS             3501..3836
                     /locus_tag="MIW95_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3150679.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3868..5190
                     /locus_tag="MIW95_001338"
     CDS             3868..5190
                     /locus_tag="MIW95_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY3150680.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            5200..6270
                     /locus_tag="MIW95_001339"
     CDS             5200..6270
                     /locus_tag="MIW95_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY3150681.1"
                     /translation="MNNTIERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6275..7462
                     /locus_tag="MIW95_001340"
     CDS             6275..7462
                     /locus_tag="MIW95_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY3150682.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVANNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7659..8531)
                     /locus_tag="MIW95_001341"
     CDS             complement(7659..8531)
                     /locus_tag="MIW95_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBY3150683.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVSYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYKQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLHLNPEVFKNDALIYTNIDEVSIYNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8925..9116)
                     /locus_tag="MIW95_001342"
     CDS             complement(8925..9116)
                     /locus_tag="MIW95_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150684.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9336..10382
                     /gene="cobT"
                     /locus_tag="MIW95_001343"
     CDS             9336..10382
                     /gene="cobT"
                     /locus_tag="MIW95_001343"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBY3150685.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10414..10974
                     /gene="cobU"
                     /locus_tag="MIW95_001344"
     CDS             10414..10974
                     /gene="cobU"
                     /locus_tag="MIW95_001344"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBY3150686.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            10984..11757
                     /gene="cobS"
                     /locus_tag="MIW95_001345"
     CDS             10984..11757
                     /gene="cobS"
                     /locus_tag="MIW95_001345"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBY3150687.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWIIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11777..12391
                     /locus_tag="MIW95_001346"
     CDS             11777..12391
                     /locus_tag="MIW95_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBY3150688.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      12458..12637
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12767..14266
                     /locus_tag="MIW95_001347"
     CDS             12767..14266
                     /locus_tag="MIW95_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBY3150689.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVGVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14305..15681
                     /locus_tag="MIW95_001348"
     CDS             14305..15681
                     /locus_tag="MIW95_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBY3150690.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15682..16653
                     /locus_tag="MIW95_001349"
     CDS             15682..16653
                     /locus_tag="MIW95_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBY3150691.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYVSSFVSMLVFTLIHVLCRMILINLII"
     gene            16685..17755
                     /locus_tag="MIW95_001350"
     CDS             16685..17755
                     /locus_tag="MIW95_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY3150692.1"
                     /translation="MKDLGHGANVGEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIGINPDFIIPGNGATEVIYLLMKSVKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLGKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRAYKKTTKELKKDLLKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17757..18644
                     /locus_tag="MIW95_001351"
     CDS             17757..18644
                     /locus_tag="MIW95_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBY3150693.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSEVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVIILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDTLCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18632..19264
                     /locus_tag="MIW95_001352"
     CDS             18632..19264
                     /locus_tag="MIW95_001352"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBY3150694.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19273..20499
                     /locus_tag="MIW95_001353"
     CDS             19273..20499
                     /locus_tag="MIW95_001353"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBY3150695.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCKERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20496..21104
                     /locus_tag="MIW95_001354"
     CDS             20496..21104
                     /locus_tag="MIW95_001354"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBY3150696.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNVVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKIPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            21094..21669
                     /locus_tag="MIW95_001355"
     CDS             21094..21669
                     /locus_tag="MIW95_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBY3150697.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21662..22414
                     /locus_tag="MIW95_001356"
     CDS             21662..22414
                     /locus_tag="MIW95_001356"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBY3150698.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22398..23519
                     /gene="cbiG"
                     /locus_tag="MIW95_001357"
     CDS             22398..23519
                     /gene="cbiG"
                     /locus_tag="MIW95_001357"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBY3150699.1"
                     /translation="MNIEGNIAIICITENGKNLAVKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIIFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSIDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPIAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23516..24241
                     /gene="cobJ"
                     /locus_tag="MIW95_001358"
     CDS             23516..24241
                     /gene="cobJ"
                     /locus_tag="MIW95_001358"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBY3150700.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24238..24990
                     /gene="cobK"
                     /locus_tag="MIW95_001359"
     CDS             24238..24990
                     /gene="cobK"
                     /locus_tag="MIW95_001359"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBY3150701.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            25009..26487
                     /locus_tag="MIW95_001360"
     CDS             25009..26487
                     /locus_tag="MIW95_001360"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBY3150702.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEEDGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26517..27422
                     /gene="hemC"
                     /locus_tag="MIW95_001361"
     CDS             26517..27422
                     /gene="hemC"
                     /locus_tag="MIW95_001361"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBY3150703.1"
                     /translation="MNIVVGTRGSTLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27412..28911
                     /gene="cobA"
                     /locus_tag="MIW95_001362"
     CDS             27412..28911
                     /gene="cobA"
                     /locus_tag="MIW95_001362"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBY3150704.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEDIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDNDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEDKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28928..29893
                     /gene="hemB"
                     /locus_tag="MIW95_001363"
     CDS             28928..29893
                     /gene="hemB"
                     /locus_tag="MIW95_001363"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBY3150705.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     NSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            30046..30624
                     /locus_tag="MIW95_001364"
     CDS             30046..30624
                     /locus_tag="MIW95_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBY3150706.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12420 a         3064 c         5670 g         9528 t
ORIGIN      
        1 atatgtatat taaattttgg aatcaaaata tactaggata taaaattctt aaataaaaag
       61 aaaaggttgt ttttataaaa aatatttttt ataccacttt aaaaaacaca ataataacac
      121 tgagggaggt ggggatatga gagaacgcat tattcttgaa acattaaaaa atagtagtgg
      181 ttatctaaac atagattatt ttgcagataa gttgggggta tctactcgaa ctatcagaaa
      241 tgaaataaaa aagatagaaa ctataaaaga atgtaatggt tttaaattag aatataagtc
      301 taaattagga tatattctaa atattaaaga ccaagataaa ttcgagcatt atctaagaaa
      361 cttaccctca gatttagttg aaaatccaga acaacggttg gagtctataa tagtagagct
      421 tttagtaaat gaaggttata aaactataga acagttaagt aaaaaatttc tagtaagttc
      481 atcccaaata aaaaatgatt taaaaaagat agatgaaaag ataaaggata cagaattaaa
      541 actagagcga aaggctcatt atggaattaa aatagaaggt tctattaaag aaattcaaag
      601 tatattggtg gatagttatt ttagagggaa tagaaatata acagagtaca ggaataagtt
      661 tatagataat ttaaaattag ctaatattag aagtacaata aaaaatgtac ttaatgaaca
      721 tgatttagaa gcaaatttaa cagaacttga ggaaatatta gcacaaatta ttatattgta
      781 tataagagtt agtatgagag tagtgggaaa tgctagcaat ttaaaattat atacagaaga
      841 tttaattata gataaattat tagataaaat ttttagagat aaaaaatata accttaatta
      901 tgatgaaaag tattatttaa aggagcttat aaaactaaag actaaagata agaaagccac
      961 tataaagaat attgataaga ataaacttca agatattatg ttcgaatttt ttagagagat
     1021 agataaaaaa tataactcta attttttaga agataaagag ttttttaact tattttactt
     1081 acatattgca tgtttaatag aacgtataaa gaagaaccac aagataataa atcctttttc
     1141 agttaaaata agtcaacagt atccaactgt ttttaattta tctattcaat tttcaaaaat
     1201 catagaaaat gagtatcata taaaaataag tcaagatgaa atagggttta tagctactca
     1261 cattgctgtt ccttttgaaa agagagaaga agctaatttt aataaaaaat ataaaatagc
     1321 aataatatgc tcttctggtg gaggtagtgc atttttgata aatttaagac ttaaagaaat
     1381 ttttccaaat gctgagataa gaaatttttc attactagaa gaaaaagctg taatagaatt
     1441 tgcaccagat ttgatttttt caataacaaa tcttttattt gaaataaatg ctccagttat
     1501 acttataaat gaaattttag acgaattgga ttatttagat attaaagaaa gtgttagatt
     1561 tgcagaccat ataggaaata tatcaagccc aaaacaatat atattaggtc tatttgataa
     1621 gaatcacttt aggtgtataa aagataaagc taagtataag gatatccttg atagtatgtc
     1681 aaaatgtata gttgacgagg gagcttgttc tccaacctat cccaaagatg tatgggaaag
     1741 agaaagctat ttgagtacta tttatacaaa tggggtggct atacctcatc caatcgagat
     1801 gacaggaaac aaaaatataa tatcagtagc tttgatacaa actgatatta tttatgaaaa
     1861 tagggtgccc aaaattattt tcatgatttc attaataaag ggaaatttag aacttcataa
     1921 gcaaatatct aaatatttaa gtaagattat ggcaaataag gatatggtag acatgctaaa
     1981 taagtcacaa tcttatgaag agtttatgta taagttaaaa atctatctgg gaggttagac
     2041 atgaatatag agttttttaa gaggttgtca gaagcagatg gtatagcatc taatgaagaa
     2101 gaggttagac atgttttatt ggaagaacta aaggcttata gtgataaaat tatctgtgat
     2161 gggctaggga gtatcatctt ttctaaaata aaagatgaga gtgcaccaaa cgtaatgatt
     2221 tgtgctcata tggatgaagt tggatttatg gtgagaagta tagacaaact tggtatgatt
     2281 cacttaataa caataggagg agtaaaacct cttgcacagt ttgtacaaaa agttagaatt
     2341 acaacaaaag aaggaaagaa aatccctgct gtcataaatg ctacatataa taatggtaag
     2401 gctgaaaata tatatgcaga tattggagca tatacagaag aagatgtata taacctaggg
     2461 attaatgttg gggatatggt aacatataca acaagttttg aggaatttac tttaccagac
     2521 cgattagtgg gaaaggcttt tgatgatagg ataggatgtt ttgtgatggg agaagtttta
     2581 aaagaactta ggaaagaaaa tctaaattgt aatattcact ttgcggctac aagtagtgaa
     2641 gaggttggaa taaggggagc aaagacatca acacagttaa taaatccaga tatagtattt
     2701 gtaatagatg tagcatgtgc taaaaatgag tttgtgagag actatacaaa tcagaaacaa
     2761 attgataaag gtgtaatgct aatgcataga gataggactc tagtccctag tagaaaaatg
     2821 atagattatg taatggaaat tgctgataaa aataatatac cacttcaaca tgatatgttt
     2881 gaatcaggtg ggactgatgg aggagaagca catctcgtaa atgaagggaa accttgtgta
     2941 gtaacttgtg taccagttag atatggtcat tgtgcatttt caatagtaag taataaagat
     3001 ttagagaaca ttataagatt gtatacacag ttaattttaa attttgatga agaacaatat
     3061 aaacatataa gaaattttat ctaaaggaga ataagaaaat gtcaacagaa gtacaaataa
     3121 tggagcttat atcaactgca ggagagagta aatcgaaagc ttttgaggct ttaaaaaaag
     3181 taaagtcaaa agattttaaa ggagcaaaat tgttaataga ggaggctcgt aaaatagata
     3241 ttgaggctca cagaatacag acagaattaa taactcaaga tttaaatgaa gatgttgaaa
     3301 gcccaacaat gagtctttta atggtacatg ctcaagacca ctatatgact tctcagctag
     3361 caaaagactt gatagaggct ttgatatatt gtctcgataa gtaattgtaa tgtagaaata
     3421 gattatttat gtaaaaattc ttttaaaagt ttagatgctt ttaagagata ataaaaatac
     3481 aaactttagg aggaattatt atgaaaatat tattatgttg tgcaggtgga ttttctacta
     3541 atatgttaat gcaaaatatg aaaaaggtgg taaaagagag tgctaaactc aatgaaaaag
     3601 attttgagtt tacagctata ccagcagatt caatagaaag tgaagttgat gattgggata
     3661 tagtacttat aggtcctcaa atagctcata aaatagactt cttaaaagga atattagagc
     3721 caaaaaataa gccttttgca gtaatagata aagatgtata tggaaaaatg gatggagcta
     3781 cagttatgaa gttggcatta ataacttata agaaacatca attagctcaa ggataataaa
     3841 aataatgagg gaaaagggag attagatatg ttttcaaaat ttgaagcatt tatgaataga
     3901 gtatttatgc ctttagctca taaggttgac aatcaacgtc accttggttc tataaaggct
     3961 ggtatggttg ctatgacacc attcacaata ttaggaagta tatttgcaat acttccagca
     4021 cttccaaata tgcttggaga aaataatcca ataagcaaat tcatacttaa caattcagca
     4081 atattggatt taccagtaag tctttccata ggtttaatag gattatatgc ttgtatgtgt
     4141 attgctttta atctggggaa ctactataaa ttatatatac caggttgtgt tgcactatca
     4201 actttttctt ttttattcct agtagcctca tttacaccag atggagcttt agaaatagga
     4261 aatttaggag ctaaagggct atttacaggt atgatagcag ggcttggttc tgtggagtta
     4321 tataattttt gtaataagaa gaatatcaca ataaaaatgc cagaaaatgt acctgatttt
     4381 gtatctaggt cttttgaatt aatacctact actataatag tttgtggaac ttttatagtt
     4441 gctcgtttta tttcattaaa agtatttgga gaattacctc cacagatact tactcaattt
     4501 ttatctccat tagtaggaag tatggataac ccatggtcag ttcttgcact taactttgct
     4561 atatgtacta tattcttttt tggaatacat tcatcagtat ttagtcctat aactagacct
     4621 ataatgatta catttatagc tgaaaacata gcagctatgc aagctggaga aacaatacct
     4681 catttcttta cagcaggagc ttcaagtgca ttctttggat ttacaggatg tggaattagt
     4741 ataggttgtg taattgcatg tatgttatca aaaaatgctt cctataagaa gattggacgt
     4801 gtatcattat tccctgcatt atttgggata aatgaaccaa tattatttgg agcaccgata
     4861 atattaaatc caataatgtt tataccacat gtatttggtg gagctataat aggtacacta
     4921 ccaatgtttt ttatgcactg gggatggtta gcaaagccag tatttgaccc tccatatgta
     4981 ggggtattct tagaaggatt tttaactaat ggagattggc attctatatt agcaaatgca
     5041 ttgcaattag tattagcagt tttattatat tggccattct ttaaaataat ggaacgtcaa
     5101 caaaataata aaaaacaaaa agaagaaact aagaatatat tctctaaaga agaagaagac
     5161 ttattggatg atttaggatt ggatttctaa tattttgtta tgaataatac aatagagaga
     5221 ttagtatcct taatctataa aagaggtgtt gacgctcttt tgattaaagc aaaaaatact
     5281 aaaagatata taggagcttt atcaggtagt ggtgtatatg tgcttgttac taaggataaa
     5341 aattatcaaa ttctagatgg cagatatatt gatgaagcag ataaaaaaac gactggtttc
     5401 ataaaaagga ttgtatctca aggttcttat ataccaacta taattgagtt attaaaagaa
     5461 ttaaatataa aaaagttagc aatagaaaac caagctatga gtatacaaga gtatctttca
     5521 ctaaaaaatg aaggttttga aatcaattta ataacaaatg aattatggaa gctaagggct
     5581 ataaaatcta aagaagaaat tgaacttatt aaaaaggctt gtgagattac agatgaagta
     5641 tttgatgagg ttatttctga aattaaaaaa gatatgactg agctagaagt atcagctcta
     5701 attcagtatc atgcattaaa aaaaggtgca agtggaatgt cttttgaaac aatagtggta
     5761 tcaggagaaa gaggtgcaat gccacatgga agacctacaa acaaaaaatt gaaagtgaat
     5821 gaagctataa caatagattt tggtgttgtt tatcaagggt atcaatcaga catgactcgt
     5881 acaatatcta taggtaaacc tcctaaaatt attaaagaga tatatgatgt agtattggaa
     5941 gcacaattat ctgcaataga gtcaataaag gaaggaacta gggcaagtga tgttgacaaa
     6001 gtggctagaa aaattataga taaacatgga tttggcgaat attttaatca tggactgggt
     6061 catggtatag gtcttggaga tggtgaagtt cccactttaa atccaaatag tgaagatata
     6121 ttagtggaag gaatggtaat gtcttgtgag ccaggtattt atattccaaa tgtaggggga
     6181 gttagaatag aagatgatat tgttataata gatggaaaag gtgttccact caataagaca
     6241 tccaaagagt ttataatatt aggagagtaa aattatgaaa tataatttta ataaagtatt
     6301 aaatcgtaaa gaaggaaatt gtagaaaatg gagcaaccat gtgattaaag aaaaattcgg
     6361 cttaaatgaa gatgcaattc ctatggattt agctgatatc gattttgagt gtgctcctgc
     6421 tataaaagaa tctatcttaa aaagagcatc agtaggagat tatagttata cttttatagg
     6481 agatgacttc tatgatgcta ttataaattg gaataaaaga agatttgatg tatatataga
     6541 aaaggattgg ataaaattaa cttttggtac tgtttcaacc cttcattata tagttcaagc
     6601 atatactaaa gaaggagagg gtgtattgat aaatacacct gcttatgacc cttttgcaga
     6661 ggctgtagca aataataata gaaagttgtg ttgtagctca ttaaaattag ataacaatag
     6721 atattattta gatttagagg atatggaaaa tcaaatgaaa tacgagaata taagattact
     6781 tatattctgt agtccacaaa atccatcagg tcgtatatgg acaaaagaag aactatatca
     6841 agtgtcagag ttatgtttaa agtataatgt aattttggta agtgatgaaa ttcatagaga
     6901 tataatattt aaagattata gatttgtatc tttgtggaat gctcatccag aaatttataa
     6961 aaatagtata atctgcgtat ctcctaataa aggcttcaac ttaggtggat taaaaacatc
     7021 atatgtatta ataagagatg taaaaattag ggagacttta ttagaaaggc taaaaagtaa
     7081 ttcaataact tctccaaatg tatttgcaat accagcaata gttgcagcat acaatgaaag
     7141 cgaagaatgg ctagatgcta tgactagtta tgtagaggag aattttgaaa tagtttatga
     7201 tttctttgaa actaatatac caaaagccaa agtgatgaag tcagactctt cttttttagc
     7261 ctggatagat gtaagagaag tatttagaaa tgaagaagaa tctaaagaat tttttagaca
     7321 tgcaaactta actatggtag ttggaagtta ttttgtgaaa gatggagatg gatttattcg
     7381 tattaatgtg gggtgtccta gagaaacatt aaatgaggct ctaaatagaa tcaaaaaaac
     7441 gtatataagt atgtattgtt aaatcataaa aaaagtatct atggtttata taaaaacaca
     7501 ttatttagtt atcaaatatt actggaattt tgtattccag taatattttt tgattaagga
     7561 atattattga ggaatattaa ataatattat ttcataccct tatattaact tatataaaaa
     7621 tattttatta ttttgggaac gggttagcta acctttatct agtgtatatt tatattgtaa
     7681 gaagcagtaa aaacttttcc cttactaagt ttattaataa acttcttatc tttataaatt
     7741 ttgttagaat ttatactatc agcaagacca taccaaggtt ctatacataa aaaaggagca
     7801 gtagaattag tttcactata ataaggagtc caaataccaa ctagagggaa atcttccata
     7861 gatatagtta catatttact accatcatta ttatatatag acacttcatc tatgttggtg
     7921 tatatcaaag catcattctt gaatacttct ggatttaaat gtaagttttt taattttcca
     7981 ataggtatta tatcatctgt aaaagaacca tttaaattga ttttttctac atcattttta
     8041 cttttaaact caaggtagtg tttagaaaaa tcattttgtt tgtaaaaagg aaggttgaat
     8101 gcaggatgtc cacctataga aaagaatata tctttagagt cagtattttt tacaatccat
     8161 tctacctcaa tactattatc atttaatgta tagtttatga ttagctcaaa agaatatgga
     8221 tacttcttta aagtggattc attatctgtt aatttataag atacaaatgt atctccttta
     8281 tctacaatct caaagtccat atctctagca aacccatgtt gagtcatgct atataaattt
     8341 tcttctatta ttgtttcatt atcaaaaagt ttacctacta tagggaacaa tataggtgat
     8401 tgtctacccc aatatttttt attaccatcc caaagaatgt ttttgtcgta ttttttagaa
     8461 aatattttag ttagttcagc accaaaactg cttgattcga taattaaatt ttcattttgt
     8521 aatatattca tttaaaatct cctttattat attttatgag tatattatac tataaagtat
     8581 aaagattatt aaattttatt tgatattata tttgaaaggt agattttagt atattttgaa
     8641 aattttgttt tgaaaatttg ggattattta agaaattttg tattataaat agaaggaaat
     8701 agataaattg tatagaataa aatatattaa gataagttaa taatgttaaa tgggaagtga
     8761 gtttatggaa aaaccaaata caaaagttgt taactttcaa gagtataaag acagaaaaaa
     8821 ccaaagagaa gtaaatagag acgaactttt aaagcttata aaaaaaattg tgacttcaaa
     8881 atagggagtt gttaaactcc ctattttaat gggtttcata ataattaaga cattctgagt
     8941 ccatctatat aatctttgaa ttgtgagaaa tctgattcac tacttaattc tatggtttct
     9001 gaaccttccg tataaaaatt cttaagatag atagtataat tattatcaac acaatcatga
     9061 taaatacatc tatatccatc ttcatacaaa ctttttaact tagaaaaatc atccacaata
     9121 tcatctcctt aaagttcttt taaatatatt tttacaaaaa gcatctaatt gtatgcaaat
     9181 tgacaatgta aaataaagga aatgaacata aaatgtagaa taatttttat atgtatgtaa
     9241 aaataaaaag aaaaagttga aatttttaga aaataagagt attttttatc taaaaagaga
     9301 gtttaaaaaa taaaaaaatg ttgggaggat aaaaaatgag tttattagaa agtatatcaa
     9361 aaaacatata ttccctagat aactcttcta ttgaaaaaac aaagcagaga ttggataggc
     9421 ttatacatcc aactggaagc ttaggaaaga tagaagatat ttgcatgcaa ttggcaggaa
     9481 tatttggcaa tgaaaatttt gacacaagta aaaaggttat aattgctttt gcgggagacc
     9541 atggtgtata tgaagaaggc gtagcacctg accctcaaaa tataacaaaa ttacagtttc
     9601 caaatttttc gaaaggtcta tgtggagttg gagtaataag taaatttgta ggggcagatg
     9661 ttgtagctgt agatgtagga ataaactgtg atgaaaaact ggatggggta ttagactaca
     9721 agataagaaa agggacatca aatatggcaa aaggtccagc tatgagtaaa caagaagcca
     9781 ttagatgttt agagataggt attgaaatag cagagcagtg tatagaaaaa gattataagg
     9841 ttattggtat tggggagatg ggtatagcaa atactactcc aagtacagca ataatatctg
     9901 taatttcagg gtgtgacccg ttagaagtta ctggtatagg tgcaggtctt aagaaagaaa
     9961 gactaaagca taaggctgaa gtaattagaa aggctattga aattaataat ccaaatccaa
    10021 ctgatggagt agatatttta tctaaagtag gaggatttga aataggctca atggcaggtg
    10081 ttatattagg ttgtagtgct aataggattc cagtagtaat tgatgggttt atttcttatg
    10141 cagcagcatt aattgcatat aagataaatc caaaaactag agaatatatg atagcatctc
    10201 atttatcagc agaatcagga actaaaagag cattagacat attaaaatta gaccctctat
    10261 taaatatgga tatgagatta ggtgagggaa gtggagctgc tttagcattt gatataatag
    10321 aagcttcaaa ctatacttac aaaaatatgg caacttttga tgaaattgac atgggaagat
    10381 aaatataaaa atttaatatg taggagtgtt ttgatgagta aaattattct agtaacagga
    10441 ggagctaggt cagggaagag taactttgca gaaaaactat gttttgacag aaataataat
    10501 acagcttata tagcaacctc tattccattt gatgatgaaa tgaaagatag agtaaaaaaa
    10561 catcaagaga atagacctag aaactggaat acttatgaaa tatatgaaga tatatattcc
    10621 ataattaagg agatatataa agaacatgaa actgttatac tagattgtgt aacattatta
    10681 gtaaataatc taatgtttga acacaatcta aatatagaag agtctagtca agaagaaata
    10741 aacaaattag aacaatacat aaaagaacaa gttgtcaaac ttattgaaga aatagaaaaa
    10801 acaaatctat actttgtatt tgtaacgaat gagttaggta tgggaattgt tcctgggaat
    10861 aaacttagta gaatttatgc tgatattgta ggtcgtataa atcaatatat agcttcgaaa
    10921 agtaatgaag tttattttgt agttagtggc atagctatga agataaagga gtagtatgca
    10981 attatgaaaa gatttatttt aatactacag tttttgacaa ggatacctat aaaattaaat
    11041 gtaggatttg atgatgaatt ttataagtca atagtatatt ttccacttgt aggttttgtc
    11101 ataggtatat tatcctattt aataggatgg atttctatgc ttttatttga accatttata
    11161 gcttctataa taataactct agctggtgtc ttaattactg gaggacttca tatagatgga
    11221 ttaggagata catttgatgc aatatatagt tatagggata aagaaaaaat gcttgaaata
    11281 atgaaagatt caagattagg tacaaattca cttctagcaa ttatgtttgt acttttatta
    11341 aaggttggat tcgtttacga tataataagt aataattcat tgtggattat aatttttatg
    11401 ccaatgattg caaggttagg agtaatgtta ttgacatata aaaccgtgac accaagagaa
    11461 aaaggaatgg gaaatttatt tataggaaaa ttaactacaa gtatgttaat aactgctata
    11521 atatacacat tattgatagt tgctcttatt actaaattta tatttttatt acctaatata
    11581 gtgttgatta aagtattagg ttcgattata gtggtttttg tatttataat attattcaaa
    11641 aaacatattt ataaaaaaat tgacggagta acaggagata tattaggctg tggaatagaa
    11701 ctttcagagc ttgtatattt aatttacata tatttgttaa tttttatgtt tttttaaagt
    11761 aaaaagtggt gataatatga taagattaat attaataaga catgcactga caaatgataa
    11821 taaaaaagga agattatcag gccatataaa tagttgtatt agtgaagagg gcaaactaca
    11881 aataaataaa attaccagat atctgagtaa tgaaaacata gataaggttt atacaacacc
    11941 atctacacgt actaaagata cagtagaaaa aatatcaaaa ttgaaattat tagaaataga
    12001 agaaaaagaa gctttaagag aaattagttt tggagacttt gaaggaagaa cttttgaaga
    12061 aattaaaatc aaatatccaa atgagtttga aaaaatgata aaagaaggta acaactatag
    12121 atatccaaat ggagaaagtt tgatagactc ttataaaaga gtggcaaaag aaattgataa
    12181 tataatttta gaaaatgata gtaatttaga tacaaagaca atacttatat gttctcatgc
    12241 aggaacaata agaaatataa taacacattt gatttctggt agttataagt atcactggaa
    12301 ctttaaaatt gataatgcat ctattacagt tttagaaatt gatggtggat ttgctgtaat
    12361 agataaaatg aattttacag acttcatata atttaatcaa aaccaaacat tacaatctgt
    12421 gtttgtaata tatctgtaaa attagcaatg aaaatagaat attagattaa aattaggttc
    12481 ttaattagat gttaaaaggg aaaaaggtta gatgcctttg cagcccccgc tactgtgaaa
    12541 ccaacgaaac tataaatacc actgtcagtt tgatgggaag gttatagaag taggatgaag
    12601 ttaagccagg atacctgcct aatttaattt aaaacaagag gtcttcgggg tgaaagattt
    12661 ttaattaata tatataaaag tcctatcttt agaagtatta ggactttttt taatttgttt
    12721 tttagtaaat tttaacaagg agggatggaa agtggggaaa aaaataatgc tacaaggaac
    12781 agcttcaaat gtaggtaaaa gtgtcttagt agcaggtttg tgtagaatat ttaagcaaga
    12841 tggttatact gttgctcctt ttaagtcaca aaatatggct ttaaattctt ttataactaa
    12901 agaaggatta gaaatgggaa gagcacaagt atttcaagca gaggctgcga aaatagagcc
    12961 aatagctgat atgaatccag ttttattaaa accttctggg aataataaaa gtcaagttat
    13021 tgtaagaggt aaagttgtag gagaaatgcc ttcttctaag tatcatgatt ataaattgga
    13081 gttagtaggt gtgttgaagg aaacatttaa tagcttaaat tcaaggtatg atattgtagt
    13141 tatggaaggg gcaggaagta atgcagagat aaacttaaaa gatagagata tctctaatat
    13201 gggtatggca gaaatagctg atgcacctgt aataatagtc ggagatattg atagaggagg
    13261 cgtatttgcc tcattagcag gaacaatgct tttattaaat gaagatgaga aaagaagagt
    13321 aaaaggtgta ataatcaata aattcagagg aaaaaaagag cttttacaag atggtgtaaa
    13381 gatgctagaa gacatcataa aagtacctgt tataggagta gtaccttata cagatataaa
    13441 aatagaagat gaagacagtg taactacgag atttaagaaa aaaatgaata aaagtgatat
    13501 acacatagaa ataattagaa ccccacatat gtcaaatttt acagatttta atatatttga
    13561 gacacaagaa gatgtaagta ttagatatgt agactatgga gaatctttag gaaatccaga
    13621 tatattaata atacctggaa ctaaaagtac tatagacgat ttaacatata taagaaaaag
    13681 tggtcttgaa tcgcaaatca aaaatttaca tagtcaaggt aaattgatat ttgggatatg
    13741 tggtggatac caaatgttag gtaaaaaatt aaaagaccct tatcatgttg aaagtgaaat
    13801 tgaagaagtt gatggaatag gtcttttaga tacagaaaca acgtttgaga aagaaaagac
    13861 tacaacacaa gtagaggcta ctattgttga atgtatcagt gaatacatga ataaccttaa
    13921 gggaaaaaaa gtaaaaggtt atgaaattca tatgggcatt acaaagcgag gtaatgatgt
    13981 aagaagttta gatttaataa ctaaaaaact tgataaagaa gtgaattata ctgaaggaag
    14041 tgtaaataaa gaggggaatg taattgggac ttatcttcat ggaatatttg atgaaataag
    14101 ctttacaaga gcaattttaa acaatataag aaagaaaaaa ggtttaggag aattagaaag
    14161 caaagtaaac tcttttaatg aatttaagga taaagagtat gataagttgg ctgatttttt
    14221 aagagaacat ttagatatag agaaaatata tgagattatg aaataatgat gacctttatt
    14281 tataatttag gtaggaggtg tattatgaaa aaaattttaa ttgctggtac taatagtgga
    14341 gtaggtaaaa caacaatatc attaggaata atgcaagctt tggttaagag aaatatgaaa
    14401 gtacagccat ataaagtagg gccagactat atagaccctt catatcacac ttttataact
    14461 ggaagacatt ctagaaattt agattcttat atgttagatg atgaaaaaat aaagtatata
    14521 gtaaataagt cctcaaaaga tgcagatatt tcagtaatag aaggtgttat gggcttatat
    14581 gatggttttg gaatagattt agataattgt acaagttcac atacttcaaa ggtattaaag
    14641 tcacctgtta tattagttat aaatggtaaa tctatggcag catctagtgc tgctatggta
    14701 cttggatata aggaattaga taaagatgta aatataaagg gtgttatagt aaataatgta
    14761 aagactaata gccattatca aataattaag agttctatag aaaaatattg taatgttgaa
    14821 gtattggggt attttcctcc aaataacaaa ttctctttag aatctcgaca tctaggatta
    14881 gttcctagtg tagaaatgaa atcactaagg gaaaaatttt ataccttagc agatgaaatt
    14941 gaacactata tagatattga cagaataatt gaaatatcag aaagcgatga atttgagagt
    15001 gattttgatt ttcaaacttt gatagaaaat aataaaataa aagaacatgt taataataaa
    15061 tcaatagcta tagcatatga taaagcattc aatttttatt atagagaaaa tatagagctt
    15121 ttagaggaac ttggtttaga aataaactat tttagccctc ttgaagatgc atcagtgcca
    15181 aatgcagatt atatttatat tggaggaggt tttccagaaa tatttggaaa agaattagaa
    15241 gcaaatgagt caatgagaac ttccatatta gaagcacata acaataatat acctatttat
    15301 gcagaatgtg gaggactgat gtacctaggt gagaaactat tgaatcaaga agaacaagaa
    15361 tttaatatgg ttggaatatt tagtggaatt agtaagatga catcttcttt aaagagattt
    15421 gggtactgta ttggtgtagc caaggaaaat actcttttat ctaaaaaagg ggaatctatt
    15481 aaagggcatg agtttcatca ttcaatattt gaaagtgatg aaaaatgtgt atattcaatg
    15541 agaaaagaaa gagatggaaa tatagtagaa gagtgggatg gtggttatag taaaggaaac
    15601 actttggcta catatcttca tactcatttt tataataact taaattgtat agaaaatttt
    15661 ttgaaaaaag gaaatgaata aatgagtatt ttatctattt atataggata tgtgacagac
    15721 ttaattgtag gagacccata ttcttttcct catccagtga gatttattgg taaacttata
    15781 aattttactc aaggtataat aagaaaggtc tctaaaaatg ataggcagtt aaaatttggg
    15841 ggatttatat tatggtttat aaccgttgga ataacatatt taacaactta tgctatagtg
    15901 aagctattta gttttaatat attattgagt attgtagtga atagttttat aatttataca
    15961 acactagcca caaaatgttt aaaagacgaa gcattaaaaa tttataatgt attaaaaacc
    16021 gaagatatag aaaaatctag gattcagcta tcttatatag taggtaggga tactactaat
    16081 ttaagtgaac ctgaaataat aagagcaact gtagaaactg ttgcagaaaa tactgtagat
    16141 ggtataattg cgcctatgtt ttatgcattt attggaggag caccacttgc tatggcctat
    16201 aaagctataa atacattaga ctcaacagtt ggatataaaa ataaaaagta taaagatatt
    16261 ggttttgcat ctgctaagat tgatgatata gccaattata taccagctag aatatctgtt
    16321 attcttatga caattggaag tttcttttta aattataact ataggaactg ttttaaaatc
    16381 tctattagag atagaaagaa tcataaaagt ccaaattgtg ctttttcaga aggcgcagta
    16441 tctggtgctc taggaataca actaggaggt accaacgttt attttgggga aaaagtatat
    16501 aagccaacaa taggggataa gcttagggag atagataaag aagatataat taaaactaat
    16561 aaaattatgt atgtatcttc atttgtatct atgttggtat ttacactaat acatgtgtta
    16621 tgtaggatga ttttaattaa cttaattata taaattattg tttgctaact taagggggac
    16681 atagatgaag gatttagggc atggtgccaa tgtaggtgaa atggcaagat tatatgggaa
    16741 aaacccaaaa gaaataattg attttagttc aaacataaat ccaaatgtat taccaaattt
    16801 agagagatac attttaaaag gattagaaga gtgtaggaat tatccagata taaattatac
    16861 aaacttgaga gaaaatatat caaaatatat aggtataaat ccagacttta taatacctgg
    16921 aaatggtgca acagaagtga tttatttgct tatgaagagt gttaaaaaaa aattggctat
    16981 aataaatccg actttttcag agtatagaag aagtgctgag ttaaataatt tagatattat
    17041 agatttagag ctagatttag aaaataactt taaacttaat atagatataa taaaagaaaa
    17101 tataaaaaga tttgatagtt tatttatatg taatcctaat aatcctagtg gtaatgtcca
    17161 agatttaaag gaacttgttc atttactagg taaacataat aaggtactaa taattgatga
    17221 aactttcatg gaatttgttg aagatgaaag taaatacagt ttggttaaat atatagaatc
    17281 aaataaaaat atatttataa ttaaagctgt gactaagttt tttggtatgc ctggtttaag
    17341 gctaggatat ggtcttacaa gcaacactga aattatgaac aaaatttatg aacacaaaga
    17401 gccatggact attaattctt ttgcagatat actatccaat tttatttttg aagataaaga
    17461 gtatattaaa aatagtaaag aatattacat agaagaaaga aaatatatgt tacaagagtt
    17521 gagaaacata agaaatataa aggtctatga tacagatgct aattttattt tgataagagc
    17581 atataaaaaa acaactaaag aattgaaaaa agatttgctt aagcaaggaa acatattagt
    17641 tagagatgcg tctaatttta taggattaga tgatagtttt ataagagtgg ctataaaatc
    17701 tcatgaagat aacaaaattc ttatagaaaa tatgaaaaat ttattgggtg attaaaatga
    17761 aatcttatgg aatatgccca gcatcttgtg gagaatttgt acaaggtata attgatgatg
    17821 aagaatattt atgttcatat gcaatagata tgtattctga agtgtatatt gaagaaaaat
    17881 tagtagatat aaatttaggg agatataagt caaggttagc aattgaaaag gtatttgaaa
    17941 aatttaattt acctaaaaaa tatactaaaa atatttcttt aaatataaat agtaaaatac
    18001 ctgttggaaa aggaatggca agttcaactg ctgatatagg agctacaata aaggcaacat
    18061 tatcattgat agataaagat ttaagtagtg aggagatttc taaattagca gcagaaatag
    18121 aaccaacaga ttctatattt atagataaaa atagtatatt taatcctcta aatggaactg
    18181 ttataaaata cctaggaaat ctgaccaatg cgaaagttat aatacttgaa ccaaacaaag
    18241 tactagatac tatgaaaatt agattgaggc aagattataa taaattaaaa gtagaaaata
    18301 aagaagtaat aaaaaaatca tttgctcttt tagaagaagg attgaaaaaa aataatttat
    18361 ctctagttgg agaggcttgt actttgagta gcttagcaaa tgagaatata gaaaaaaaag
    18421 agtatttaaa tgaaataata aaaatatcaa aaaaatatgg agcatatgga gtaaatattg
    18481 ctcacagtgg aactgtagta ggtattttaa ttgataagtc tatgaatgat aaaaagatga
    18541 tagatacctt atgtgaatct aacataaatt ctgtctataa taaaatttat acacaaaaca
    18601 taattaatgg aggaattaag ggggaaatag aatggaatac ataaagaatc ctatgaaaat
    18661 agaagaaaaa agttttgaat tgatacaaga aataatagac gaaattagac cagattataa
    18721 atttaagaat aatattgaag aaaaaataat aaaaagagct atacatacta ctgctgattt
    18781 tgattactta gatatattaa aaatatcaga agaagcagtt gatagtataa tagatgcatt
    18841 aaaaaataat gcaagtattt atactgatac aaatatggct cttagtggta taaataaaag
    18901 aaagttagaa tcattaggat gtaagtataa atgtttagtg gcagatgatg aaactgtaaa
    18961 attagctaaa gaaaaaggaa ttactcgttc tatggcagca gtagaaattg cagctaaaga
    19021 agaaggaaga aaaatattcg ttttaggtaa tgctccaaca gctttatata aagttatgga
    19081 gatgaaatca gaaggtagat tagatttaga tgcagttata ggagttcctg taggttttgt
    19141 aggagcacaa gaatcaaaag atgaagttga aaaaacagat ataccatata taatttctaa
    19201 aggtagaaaa ggtggcagta atttagcagc agcaatagta aatgctatat tatatactat
    19261 gtaggtacct atatggaaga atatgtatat atagatggaa aaaaatatag aagaggatat
    19321 actacaggtt catgtgcaac aggtgcatct aaggctgctg tatatatgtt gattacaaaa
    19381 aatagaatca atactataaa tatagatact cctaagggga taccattact actaaaagtt
    19441 gataatataa atatttctga tacatttgtg gaatgttcca taaaaaaaga tggtggagat
    19501 gatatagatg caactcatac catggatatt tatgcaagag ctgaaattgt agctaaaaat
    19561 gataagaaca aagggtatct tactctaaag gatatagata gtttaagcac caatagtgag
    19621 tgtaaaagtg agctctataa gtttattagg gtttatggcg gaactggtat tggtgtggtt
    19681 acgaaaaaag ggcttagtgt ggatgttggt aaacctgcta taaatccgac tccacttaaa
    19741 atgataaacc atgaaataag aaagcttata ggagataatt ttgaatctat acttggaaat
    19801 gataaagttt taaaaataac aatctttgct ccacaaggag aaactgtagc aaaaaaaaca
    19861 tttaatccaa gattaggtat agttggagga atatctataa taggtacaac tgggattgtt
    19921 gaacctatga gtgatgaggg ttggaagaag tctctttcta tagaacttca gatgaaaaaa
    19981 gaacaaggct tagataaaat aatattggtt ccaggaaatc atggagaaca gtttataaga
    20041 gaaaaattaa acttagatat taagtatgta gttagggtaa gtaattttat aggctatatg
    20101 attaaagaag ctcaaagaat aggatataaa aaaatcttaa tggcaggtca tataggtaaa
    20161 tttataaaag tatctgctgg tatttttaat actcatagta aggtagcaga tgctagaagt
    20221 gagatattag tggcaaactt agctttaatg ggagctagat acgaattttt aaataaaata
    20281 aatcaatgcg taactactga agaagctgta gagctaataa ataatagtga atatagagaa
    20341 gtatataata tattaagtaa taaatgtaag gagagagtaa agcaatactt gaatgaagat
    20401 tcagatgata ttgatgtgga agtaatcata ttttcaatgg ataagtcttt attaggtaaa
    20461 tcggataata cagatgattt agtggaggtc tttatatgat aaacataata ggtctaggac
    20521 ctggtaactt agactatata acaaaaaaag gtgaaaattt aatttccact tctgatgtat
    20581 taattggtgg taaaagaaat ctagagtcta taaaaaattt tgaaggagaa aaaatagttc
    20641 tggattctaa tttaagagaa attatagagt acataaataa caataaagaa aagcaaatat
    20701 caataatagc atctggggac ccattaatat atgggatagg tagatatcta tctaaaaata
    20761 ttgataataa gatgttaaac gtggtttctg ggattagttc attacagtac atattttcaa
    20821 aaatatatgt agatatgaat gatgtatata ttacaagtag tcatggcaaa ataccagatt
    20881 ttgattacat attatcacat aaaaaagtat gtatggttac agattcaaag ataggaccaa
    20941 aacaaataag tagagaaata atagatagaa atctaaataa aattattgtt gtaggagaaa
    21001 atctgtcata tgacaatgaa aaaataacaa tagcgaaacc agaagagata ataagaatgg
    21061 acaattttga tatgaatgtt gtggtgattt tagatgagga atagtgaatt tataacaggt
    21121 aaagttccaa ttactaagga agaggttaga gcaatatcaa taagtaagtt aaatttagta
    21181 aatgctaaaa acctcataga cataggggct ggtacaggaa gtgtaagtgt tgaagctgca
    21241 tataattatc ctaatttaaa ggttatatct atagagaaaa atgatgacgc tatagagctt
    21301 atagagaaaa atataaaaaa attcaactta aaaaatatag aagtgataaa aggatatgct
    21361 ccaattaatc tagggttaaa tgttaaagtt gagtctattt ttcttggggg aacaggaaat
    21421 aatctggaag aaataataga atggtctaaa aaaaatctcg ttacaggagg aagacttgta
    21481 gctaatttta taataataga tacatttaat caaactttaa aactgcttaa aaagcatgga
    21541 tttaaagaaa tagatgtatg tgttttgaat gtttctaaac tagagaaact aggaaaaggt
    21601 gaatatttta aaccactaaa tcctatatat ataatatctt gtgaaaaagg ggaaaatgat
    21661 gatgaataaa gtacattttg taggagcagg acctggagat aaagagctga taacattaaa
    21721 aggatacaaa ttattaagta atgcagatgt agtgatatat gcaggttcac ttgttaatcc
    21781 tgaacttttg gaatactgta aagaagattg ccaaatacat aatagtgcac atatggattt
    21841 acaagagata atagatgtta tgagagaagg tatagaaaat aataaatctg ttgtaagact
    21901 gcaaacaggt gatttttcta tatatggttc aattagagaa caagtggaag atttaaataa
    21961 attgaatata gattatgact gtactcctgg agtaagttct tttttagggg cagcatcatc
    22021 tttaggggtt gaatatacag ttccagaaat ttctcaaagt gtaattataa caagaatgga
    22081 aggcagaact ccggttccag agaaagaatc aatacaatca tatgctaagc atcagacatc
    22141 tatggtaata tttttatctg ttcaagaaat tgagaaagtt gtgtctaaat tactggaagg
    22201 tggatatcca aaagatactc ctatagcagt tatatacaaa gcaacttggg cagatgaaaa
    22261 aatagttaaa ggaactttaa gtgatatagc tgtaaaagtt aaagaaaaca acataaataa
    22321 gactgcttta ataatggttg ggagattttt aggagaagag tataataatt caaagttata
    22381 tgataaggat ttcaaacatg aatatagagg gtaatattgc tataatctgt attacagaaa
    22441 atggaaaaaa tttagctgta aagataaatt ctctagtaaa taactgtgtt atatatcaag
    22501 taaaaaataa aaaaagtaat ttagttgttg aagaaacatg tgtacataca gttcaaaaga
    22561 agttaagtga ttttgtagga gaaatattta gcaaatttga ttacataata tttataatgg
    22621 ctacaggtat agttgtacga agtatagcac catacataat aagtaagttt agtgaccctg
    22681 caattttggt aatggatgaa aaaggcaaaa acgtaataag tttattgagt ggtcatattg
    22741 gaggggcaaa cagcttgaca cagtacataa gtagtcttat tgatgctaat ccagtaataa
    22801 caacagctac tgatataaat caaaaagctt ccttagatat gatagcaaaa aaactaaatg
    22861 cacatattga taattttaga gaaagtgtaa aagaagtaaa ttctatgcta gtgaataata
    22921 aatgtgttgg attatatgtt gatgaaggat atcaagttga tactagggga tttaaactta
    22981 tagaaagtaa ggatgagtta agagatagct tttcaaacac atatatagaa aaattagttg
    23041 tagtaagcaa taaaataaat tgtattgaag atattgagaa tttaaactta gataataaat
    23101 ttgaacattg ctatagtact aaaaaaataa taaaggtaat tccaagagat atagtgatag
    23161 gaattggctg tagaagaaac acagatagtg agctgatgta taattcactt gaagagtttc
    23221 ttaataaaca aaatatagat attagagcaa taaacacagt tggaagtata gaactgaaaa
    23281 aagacgaaaa agcaataatt gaattatcaa aaatactaaa agtgccattt aaaacatttt
    23341 caatagatga aatatctaag gttgataatc tttttgataa atctgatttt gttaaaaaaa
    23401 atgtgggcgt gtattcagta gcagaaccta tagcatatct tttgagcaaa ggtaatttgt
    23461 tagttgaaaa acataaatat aaaggaataa ctttttcagt ggggagaata aaaatatgat
    23521 atacgttgta ggaattggac caggaagtaa agatacaatg actttagaag caataaaagc
    23581 aatagaagat tctgaggcta ttgttggata taagacttat ataaaactaa tagaagaatt
    23641 tatacaaggt aaagaaataa tccaaaatgg tatgaggcaa gaagttgata gatgtaaaca
    23701 agcagtagag gaagcgaaaa aaggaaaaaa agtagcagtt gtaagcagtg gtgatgctgg
    23761 aatatatggg atggcaggtc tcattttaga attaatatct aaagaagatg aagatataaa
    23821 agtaaaggta attccaggtg ttactgcaag tattggtgca gcagctatac ttggagctcc
    23881 tattatgcat gatttctgtc atataagttt gagtgattta atgactccgt gggaagtaat
    23941 tgaaaaaaga ttgagattgg ctgctgaagc cgattttgta atttgtttgt ataatccaag
    24001 aagtaaggga agaagtgaac atctaagcaa agcatttaaa ataatgggtg agtttaaagg
    24061 tggagataca cctgttggag tggtaaaaga tgtaggtaga gaaaaagaag aaaagtttgt
    24121 atgtactttt gaaaatatgg actttgaaaa agtcgatatg actacaatgg ttataatagg
    24181 aaataagtct acctatatta atggtgaatt gatgataact ccaagaggtt atacagtatg
    24241 attttaattt taggaggaac ttcagatagt ttatctatat gtgataagat aaatgaactg
    24301 aaaaatcaac catatatcct ttcagttact acagattatg gaagggaact tgcacaacaa
    24361 catgcagaga atgtaatttt aggtaagctt ggaaaagaag atatgctaaa atttataaaa
    24421 gataataata tagtaaaaat aatagatgcc actcatcctt atgcagttga agtatccaaa
    24481 actgctatta cttgtgcaaa attattgaat atagattata taaggtttga gagaaaatct
    24541 ttaattgaag aaatagatta tgataataaa tttatagtag atacaattga agaagcctgt
    24601 gaaatagcaa ataaaatagg gacaaatata ttcataggaa caggaagtaa aaatttgaac
    24661 agatttatag aggaaataag tgataaaaat ttagttgtta gagttcttcc aacgagtgaa
    24721 gtcatattaa gttgtgaaaa attaggttta agtgcagata atatagtagc tatgaaaggt
    24781 ccatttagcc agtatataaa tgaagaaaca tataaacatt acaatataga tttagtcata
    24841 acaaaagaaa gtggaatagc tggtgggttc ttagagaagg taaatgcatg taaaactttc
    24901 aatataccag taataattat aaaaagagaa tcgattaact acgaaaatgt atttaatgac
    24961 ataaataaaa ttaaagacat acttgtgtaa tttttaggag gagaatatat gaagaaagcc
    25021 ttattagtag tgagttttgg tacaagctat catgaaacga gagaaaagac aatagatata
    25081 tgtgaagata aaataaaaaa ttcgttaaaa agccatgatt tttttagagc ttatacatca
    25141 aatatgataa taaataaaat aaaaaaaagg gatggaatag aaatagataa tccaatacag
    25201 gctttagata aaatatatga acaaggatat gatgaagtaa taatccaaac acttcatatt
    25261 atatgtggcg aagaatttaa caaattaaaa gaacaagtag atggatattc ttctaaattc
    25321 aaaaagattg tattaggaag acctttgctt acacatattg aagattatca ggaagctgta
    25381 gaggcaatta agcatcaaat tcctcatatg gaaagtaatg aagcagtagt atttatggga
    25441 catggaacac ttcatgaatc acattcagcg tatccagcat tggaatacat gcttagggat
    25501 aatggaataa atgcttatgt tggtacagtt gaagggtatc cagaaataga acatgtaata
    25561 agaagattaa aagaaggaaa tataaaaact gtaaatttga tgccatttat gttagttgca
    25621 ggagaccatg ctataaatga tatggcagga gatgaagaag attcttggaa aacaatatta
    25681 gaagaagatg gatttgacgt aagagttcat ttaaaaggac taggagaaaa ttcttatata
    25741 caagataaat ttgttcgtca tgctgtaaaa tgtgcagaga atgctaagtt ctatggtata
    25801 ggagcagggc caggagatgg ttctttactt acgataaaag cggttaatac tttaaagaaa
    25861 ctggatattc tatacactcc agaatcaaaa aagggtggag atagtctagc tttatcaata
    25921 gttagtgaat atttacctga atcagtggaa ataaagtcta ggcatttccc aatgagtttt
    25981 gatgggaatg aaaaaagttt agcttgggac aatgtagcac aagaaattat atcagatgtt
    26041 aatgatggta aaaatgtagg atttgttaca ttaggagacc ctatgattta tagtacatat
    26101 gtatatataa tgaaaagaat attaggagag gtagaagtcg aaactatacc aggaatttca
    26161 tcattttcca atatagcatc taatcagaat tttccattag tcatggataa agaagcttta
    26221 gtggtaattc cttgtactat ggaagatgat aagatagagt atgctttgca aaattataat
    26281 tcaatagtat tgatgaaggt atataaaaat tttaaagaaa taataaaaaa attagaaaag
    26341 tatgatttaa tagaacatgc tattttagta agtaattctt cccaagaaag cgaaaatgtg
    26401 tttacagatt taaaagaagc gcaccttgaa gataagatat cttatttttc tactatacta
    26461 gtaaataaag ataataaaat aaaataggtg aaagcaatat ttaaaggaga cttaatatga
    26521 atatagtggt tggaactagg ggtagtactc tagcgcttat acaaacagag tgggtaataa
    26581 atgaattaaa aaagaagtat ccagaaatta gttttgagat taaaatcata aagacaaaag
    26641 gagatttaat tcaaaatgtt tcactggata aaataggtga taaaggattg tttgtcaaag
    26701 aaatagagca acaattatta gatggaaaaa tagacatagc agtacatagt atgaaagata
    26761 tgccttctta tttagcagat ggactaaaat ttgctcatac gcctaagaga gaagacccaa
    26821 gagatgttct aatacttaga gaaggatata aaaatttaga tgatttgcct catggagcag
    26881 taataggaac aggaagtaaa agaaggaagt ttcaattatt gaaacaaaga cctgatttaa
    26941 atatagtaca agttagaggt aatgtggaaa ctaggatacg aaaaataaaa gatgaaaaca
    27001 tgcatggtat tgtgttggca gcatcaggaa taattagagc taatttgcaa gataaaataa
    27061 gttcttattt acctgttgat gttgttattc cagcacctgc ccaaggagct ttagctatag
    27121 aaattagaag caatgatagt acgatagagg gaatagtaaa ttcactgaag gatgagaata
    27181 cagaaatcca aatattggct gaaagaggtt ttttagatgg tgtaaatgga agttgtcata
    27241 ttccaatggc agcttattgt gaaataatcc aagataaaat acatcttact ggcttatatg
    27301 gagattcaga aggaaaaaaa gtagtaatca agtctataga tggagatatt tcttctccta
    27361 gagagttagg tttaaagtta gctaaacttg tattgaagga gtatgagaat tatgaagggt
    27421 aaggtttact tagtaggggc aggaccaggt gattataaac tcatgacttt aaaaggttta
    27481 gagtgtataa gaaaatcgga tgtaatagta tatgatagat tagcaaatag tagttattta
    27541 agagaagcta agcctgattg tgaacttata tatgtaggca aggcatcaag taatcatatt
    27601 ttgccacaag aagatataaa tagaattata gcagaaaaag ctaaagaagg aaaaattgta
    27661 actcgtctta aaggaggaga cccttatgta tttggcagag ggggagaaga agcagagact
    27721 ttagtagatg aagatataga atttgaagta gttccaggta taacttctgc tataggaggt
    27781 ttatgttatg cgggtattcc tataacacat agagaccatg cttcgtcatt tcatgtaata
    27841 acaggtcatt tgaagggtga tgatagtgga gaattaaact ggaatgctct tgcaaataac
    27901 aaaggtactc ttgtgttttt gatgggaatt tcaaacctag agaaaataag tgaaaactta
    27961 atgaaagaag gaaaagataa ggatacacct gttgctctta taagttgggc aactagatat
    28021 aatcaaagag ttgttacatc tacacttgag aatgtttatg aaactgctat taaagaagaa
    28081 gtaaaaccgc caactcttat agtagttggg agtgttgtag gacttagaga aaagttgaac
    28141 ttctttgaaa gtaagccttt atttggaaaa agtattgctg taactaggtc tagaaatcaa
    28201 aatagtgtat tggtagaaaa aataatggat ttaggtggaa atcctataga aatacctact
    28261 attaaggtag aaaaaataca aaataatatt aacttagaaa atgaaataaa aaatataaat
    28321 aagtataatt atttaatact aactagtaaa aatgccgtag aaatcttctt tgaaaagata
    28381 tatgaaatga attttgattt aaggatatta tctaatttaa aggtatgtgc tataggttct
    28441 gctacatcaa atgagttgaa aaaaagagga attatagcgg atatagtacc aaaaaaattt
    28501 gtagcagagt cactgtatga agagttagct cctattttaa atgataacga taatatactt
    28561 atacctagag cagaaaatgc tagagattat ctggttgaca aacttaaggc tatttgtgaa
    28621 gtaacagaag tacatatata tagaacagta atagatgagg ataaaaaagg tgagatatta
    28681 gatattttaa attctaatga tatagattat ataacattta ctagttcctc aacagtaaat
    28741 aatcttgttg aaattatagg aaaagaaaat attaaaatat tagaaaaaat aaaaacacta
    28801 tctataggtc ctataactac agagacaata aatagtttgg gaataaaatt gtacaaagaa
    28861 tctgctacat ctactataga ttcgttagta gatttaatta taaaaaatta attttggagg
    28921 cttgaatatg ttaagaagac caagaagact aagagcaagt aaagcaatca gaaatttggt
    28981 aagagaaact aaggtgaatg tagaagattt aatatatcca ctgtttgtag tagaggggga
    29041 aaatataaaa gaagaaatca attctttacc agatgtatat catttatcaa tagacatgct
    29101 agaagacgaa ataaaagaaa taagtgagtt gggaattgaa catataatgt tatttggtgt
    29161 tccagatgaa aaagatgagt gtgcttcaca agcttttaat gataatggaa tagttcaaaa
    29221 ggcaattaga aaggtaaaag aaataaattc agatataaat gtaataacag atgtctgtat
    29281 gtgccaatat acaagtcatg gacattgtgg gattttaact gatactggtt atgttgataa
    29341 tgataaatca ttggagtatt tggctaaaat atctgtaagc catgctaaag caggagctga
    29401 tatggttgct ccatctgata tgatggatgg aagaatacaa gcaataagag aagctttaga
    29461 tgaaaatgga ttagaaaatg ttggaatcat ggcttatagt gttaagtatg cttcaaatta
    29521 ttatggacct tttagagagg cagctcattc agcaccatct tttggagata gaaaaacata
    29581 tcaaatggac ccagcaaata gcaatgaagc tataatagag actgaacttg atatattgga
    29641 aggtgcagat atacttatgg ttaaacctgc attatcatat ttagatatta taagaagagt
    29701 taaagataat tttgacttac ctctagcagc ttataatgta agtggagaat actctatgct
    29761 taagtttgct gtgaaggaag gtttattaaa tgaagatgta attcttgaat caattacatc
    29821 tataaaaaga gcaggagcag atataataat aacttatttt gctaaagaca ttgctaaact
    29881 gttgaaaaaa taaattctag tagtataaag aatatcaaaa agtataggaa ttgtaattaa
    29941 ctattataca gtagttttgt ttagacattt attgaagaat aatagaaact aatttacaat
    30001 ttctatactt tatttatgta actaaataaa atttggaggt agaagatgct atatcctatt
    30061 aatttaaaat tagatgagtt agatgtagtt ataattggtg gtggagaagt tgcatatagg
    30121 aaatgtaaaa attttttaga gtttaacaaa aatgttacta tagtttctaa acaaatttta
    30181 aataaatttt atgatttaaa aggaaatata aaaataataa aagatgatta taaagaaata
    30241 tatataaaag atgcttctgt aataattgct gccacaaata atagagagtt aaatatggaa
    30301 ataggattat attgtagagc aaaaaataag ctagtgaatg tagttgataa tatagaaata
    30361 tcgaacttta ctgttccatc atatataaaa agaggagatt tacttataag tgtgtctact
    30421 gggggcaaga gtccttcatt atcatcaaag ataaaaaaag aaattgaaga gagatatact
    30481 gaagattatg aggagtattt aaatgtatta ggagatataa gaaaaaaggt tataaaaaaa
    30541 tacgaagata aaagtaaaag aaaagatgtg cttaatatgt tggtaacact tgacctagaa
    30601 gaacttaaga agtttgatat ataaaaaata aaataaaaaa tgttaaaaag gtgttgacgg
    30661 attattttta agatgatata gt
//
LOCUS       DAGQWR010000035        30581 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.035, whole genome shotgun sequence.
ACCESSION   DAGQWR010000035 DAGQWR010000000
VERSION     DAGQWR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.035"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            48..123
                     /locus_tag="MIW95_001365"
     tRNA            48..123
                     /locus_tag="MIW95_001365"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:81..83,aa:Gln,seq:ttg)
     gene            132..207
                     /locus_tag="MIW95_001366"
     tRNA            132..207
                     /locus_tag="MIW95_001366"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:165..167,aa:Lys,seq:ttt)
     gene            210..298
                     /locus_tag="MIW95_001367"
     tRNA            210..298
                     /locus_tag="MIW95_001367"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Ser,seq:tga)
     gene            302..377
                     /locus_tag="MIW95_001368"
     tRNA            302..377
                     /locus_tag="MIW95_001368"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:335..337,aa:Phe,seq:gaa)
     gene            384..457
                     /locus_tag="MIW95_001369"
     tRNA            384..457
                     /locus_tag="MIW95_001369"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:418..420,aa:Met,seq:cat)
     gene            472..548
                     /locus_tag="MIW95_001370"
     tRNA            472..548
                     /locus_tag="MIW95_001370"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:506..508,aa:Ile,seq:cat)
     gene            566..642
                     /locus_tag="MIW95_001371"
     tRNA            566..642
                     /locus_tag="MIW95_001371"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:600..602,aa:His,seq:gtg)
     gene            651..726
                     /locus_tag="MIW95_001372"
     tRNA            651..726
                     /locus_tag="MIW95_001372"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:684..686,aa:Lys,seq:ttt)
     gene            734..807
                     /locus_tag="MIW95_001373"
     tRNA            734..807
                     /locus_tag="MIW95_001373"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:766..768,aa:Cys,seq:gca)
     gene            815..891
                     /locus_tag="MIW95_001374"
     tRNA            815..891
                     /locus_tag="MIW95_001374"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:849..851,aa:Arg,seq:acg)
     gene            904..979
                     /locus_tag="MIW95_001375"
     tRNA            904..979
                     /locus_tag="MIW95_001375"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:937..939,aa:Val,seq:tac)
     gene            1055..1468
                     /locus_tag="MIW95_001376"
     CDS             1055..1468
                     /locus_tag="MIW95_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150707.1"
                     /translation="MYIVIGNEIVDSEELKIIIDENSKFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLSSLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            1777..5208
                     /locus_tag="MIW95_001377"
     CDS             1777..5208
                     /locus_tag="MIW95_001377"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBY3150708.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5450..7378
                     /locus_tag="MIW95_001378"
     CDS             5450..7378
                     /locus_tag="MIW95_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBY3150709.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            7831..8295
                     /locus_tag="MIW95_001379"
     CDS             7831..8295
                     /locus_tag="MIW95_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBY3150710.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            8302..8796
                     /locus_tag="MIW95_001380"
     CDS             8302..8796
                     /locus_tag="MIW95_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBY3150711.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            8812..9837
                     /locus_tag="MIW95_001381"
     CDS             8812..9837
                     /locus_tag="MIW95_001381"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBY3150712.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            9824..12271
                     /locus_tag="MIW95_001382"
     CDS             9824..12271
                     /locus_tag="MIW95_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBY3150713.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            12451..13824
                     /gene="radA"
                     /locus_tag="MIW95_001383"
     CDS             12451..13824
                     /gene="radA"
                     /locus_tag="MIW95_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBY3150714.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDENIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            13827..14897
                     /gene="disA"
                     /locus_tag="MIW95_001384"
     CDS             13827..14897
                     /gene="disA"
                     /locus_tag="MIW95_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBY3150715.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            14986..16080
                     /locus_tag="MIW95_001385"
     CDS             14986..16080
                     /locus_tag="MIW95_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBY3150716.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            16209..18386
                     /locus_tag="MIW95_001386"
     CDS             16209..18386
                     /locus_tag="MIW95_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150717.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDMSTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSENGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKTYESVKDNIEKNIDVYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(18572..19417)
                     /locus_tag="MIW95_001387"
     CDS             complement(18572..19417)
                     /locus_tag="MIW95_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3150718.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCTYQDTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSGKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            19634..20800
                     /locus_tag="MIW95_001388"
     CDS             19634..20800
                     /locus_tag="MIW95_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBY3150719.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNIVLDTHQYLMLAESDGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            20822..22468
                     /locus_tag="MIW95_001389"
     CDS             20822..22468
                     /locus_tag="MIW95_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBY3150720.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYIHSSGFDASILHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWRPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNVLGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIADESKDVD"
     gene            22565..23938
                     /locus_tag="MIW95_001390"
     CDS             22565..23938
                     /locus_tag="MIW95_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3150721.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMFFYKLDKEYSTI
                     LNELNTK"
     gene            24383..25336
                     /locus_tag="MIW95_001391"
     CDS             24383..25336
                     /locus_tag="MIW95_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBY3150722.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            25374..26336
                     /locus_tag="MIW95_001392"
     CDS             25374..26336
                     /locus_tag="MIW95_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBY3150723.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            26361..27347
                     /locus_tag="MIW95_001393"
     CDS             26361..27347
                     /locus_tag="MIW95_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBY3150724.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            27360..28406
                     /locus_tag="MIW95_001394"
     CDS             27360..28406
                     /locus_tag="MIW95_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBY3150725.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYSGKIRKCDVEKLTAKETIVSNNTSKYSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            28573..30303
                     /gene="lpdA"
                     /locus_tag="MIW95_001395"
     CDS             28573..30303
                     /gene="lpdA"
                     /locus_tag="MIW95_001395"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBY3150726.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEVPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
BASE COUNT        12353 a         3306 c         5832 g         9090 t
ORIGIN      
        1 ggcgtgtgtc cggggttcga atccctgtgc gctcaccaca tatatattgg ggattagcca
       61 agtcggtaag gcacatgact ttgactcatg tatgcgtagg ttcgagtcct gcatccccag
      121 ccaaacagta tggtctatta gctcagtcgg tagagcacct gacttttaat cagggtgtcc
      181 cgcgttcgag tcgcggatag atcaccaatg gagaggtgtc cgagtggttt aaggagctgg
      241 tcttgaaaac cagtgacttc gaaaggggcc gtgggttcga atcccaccct ctccgccaaa
      301 tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg gtggttcgat
      361 tccgcctctg ggcaccaaaa tgtggcggta tagcttagtt ggctagagcg ttcggttcat
      421 acccgaaagg tcataggttc gactcctatt accgctacta aaatttaatg tgggccatta
      481 gctcagttgg ttagagcgcc cggctcataa ccggtaggtc tggggttcga gtccctgatg
      541 gcccaccaca ttaaattaac ttatggtggg tatagctcag ttggttagag cgccagattg
      601 tggctctgga ggtcgtgagt tcgactctca ttatccaccc caacttatat ggtctattag
      661 ctcagtcggt agagcacctg acttttaatc agggtgtccc gcgttcgagt cgcggataga
      721 tcaccaaaaa ggcggcgaca tagccaagtg gtaaggcagt ggactgcaac tccttgatcc
      781 ccagttcgaa tctgggtgtc gcctccaata aaatgcgctc atagctcaac tggatagagt
      841 gtctgactac gaatcagaag gttaggggtt cgagtccctt tgggcgcacc atatgaatag
      901 cgagggatta tagctcagct gggagagcac ctgccttaca agcagggggt cacaggttcg
      961 agccctgtta atcccaccag tgcaaacctc tagtaaaact actagaggtt ttatttttga
     1021 ttgaaaaagt ttttatattt aggaggtaag aaatttgtat atagtaatag gaaatgaaat
     1081 agttgatagt gaagaactta aaataattat agatgaaaat agtaaattta aagttgaaaa
     1141 agatttaagt aaaagcacaa aaagagaaga tgtaatagca tatcagttaa gtatagattt
     1201 aaattaccta gattctctta taaatgagca atgtaatttg tccagtttat ctgatgaaga
     1261 gaaatttgat gaatatatga ctttgtctga tgaacttgca ctggatttag aagagttaat
     1321 gccaaaatat acaataataa atgctagagc atataaatta gacgaggtag atggaattgt
     1381 aaaaataata ctagctgtag cgtatgcaga tttagggcac ttaaaacttt ctgatgtagt
     1441 aaagaggcta tctagacaag tagattagga tatgaatatg aactatatta aaaaaatgta
     1501 taaaatctta cgtaaagatg aaaataatta tgtaaacata gaaaaaaaca gtataaaatc
     1561 aagtcaattg tagaaatttt cacataaatt catctgaaat tctataaaaa atgccttaat
     1621 tttaatgagg taaatcgagt ttacacagtc taaaaattac ttatatttat aaatttatct
     1681 tattaattaa ggaactttct taaacatctt caaaaataaa gtattttagt ctataatctt
     1741 taatattaca caaacaataa aggagaagaa tagagtatgc ttaaaaagtt taataaaata
     1801 ctagtggcta atagaggcga aatagctata agaatattta gagcctgttc agaattgggg
     1861 ataaaaagtg ttggtatata cagtaaggaa gataagtatg gattgtttag aacaaaagca
     1921 gatgaatcat atttgatagg agaaggtaaa gggccaatag atgcatattt agatatggat
     1981 ggaataatag acctagcaaa gagaaaaaaa gttgatgcta tacatccagg atatggtttt
     2041 ctagcggaga atgcagaatt tgctcgtaaa tgtgaagaga atggaattac ttttatagga
     2101 ccatcatcta aggtaatgaa tatgatgggg gataagatta actctaaaaa aatcgcaaaa
     2161 gaagttaatg ttcaaactat accaggagta gaaaaagcaa taagaagtac tgaagaagca
     2221 aaggaagttg caaacaaaat aggatatcct gttatgataa aagcatctaa tggcggtggc
     2281 ggtagaggta tgagaattgt acatcgcgaa gaagacttag aacttgaata tgaaactgct
     2341 tgtagtgaat caagaaaagc ttttggagaa gatattatat ttatagaaaa atatatagct
     2401 gatccaaaac atatagaagt tcaaatatta ggtgataatt atggaaatat agttcactta
     2461 tatgaaagag attgttcagt tcaaagaaga catcaaaaaa taatagaata tgcaccagct
     2521 ttttcgttag atgataaagt aagaaaagag atatgtgaag atgcagttaa gttatcaaaa
     2581 catgtgggtt attctaatgc gggaacatta gaatttttag tagatgcaaa tggtgggcat
     2641 tattttatag agatgaacac aagagtacag gttgaacata ctgtaacaga gatggttaca
     2701 ggaatagata tagttcaaag tcaaatacta atagcccaag gttacagctt agatagtgaa
     2761 gaaattaata taaaatcaca agatgatgtg gaaataagag gttactctat ccaatgtaga
     2821 ataactacag aagaccctaa gaataaattc atgcctgata caggtaaaat tcaagtttat
     2881 agaactggtt caggatttgg tataagacta gatggaggaa atggatttac aggggcaaac
     2941 ataagtcctc attatgatag tttattggtt aagacaatat cttgggatag aacattccaa
     3001 ggagcaataa ataaaactat aagatctata aaagaactta gagttagagg tgttaaaact
     3061 aatgtaggat tcctagttaa tgttttaaat aatcctatat tctcaaatgg taaatgtagt
     3121 actaagttta tagatgaaaa tccagattta tttgaaataa cagaaagtaa agacagagga
     3181 acaaaattac ttcaatttat aggagatgta attgtaaatg ataatgcttg taaagaaaaa
     3241 ccactatttg atgcattaca tgacccaaga atggataagg atggttcaaa atcagaagga
     3301 agtaaaatac ttttcgataa gttaggtaaa tctgcatata tagaaaagat aaaaaatgat
     3361 aagaaattac tattaacaga tacaacaatg agagatgcac atcaatcatt attagcaaca
     3421 agaataagaa catatgactt attaaaagct gctaagccaa cagaaaaata tcaaaaggat
     3481 ttattctcac ttgaaatgtg gggaggagct acttatgatg ttgcatatag atttttaaaa
     3541 gaatctcctt ggcgtagatt acaaaagcta agagaagaaa taccaagcat aatgttccaa
     3601 atgttgctta gagcatcaaa tggagttgga tataaaaact atccagataa tgtaatagaa
     3661 gaatttacaa aagaatctgc tagacaaggt atagatgtat ttagaatatt tgattcatta
     3721 aactgggttg aaaatatgaa accatctatt aatacagctt tagaaactgg aaaaattgtt
     3781 gaagcaacta tgtgttatac aggtgatata ctagataaga ctaaaactaa atacaatcta
     3841 gaatattata ttaaaatggc acaagaatta gaaagcttgg gagcagatat aatagcaata
     3901 aaagatatgt caggtcttct taaaccatat tcagcttaca cactagttaa agagttaaag
     3961 aaaaatgtaa aagcaccaat acatttacac actcatgata caagtggaaa tggtgtagca
     4021 acttgtttaa tggcgtcaga agctggagta gacataattg atgctgcatt agaatctatg
     4081 gcaggactta caagtcagcc atcgcttaat gctatagtag aggcacttaa gaatacagag
     4141 agagatacag gaatagattt atttggatat gatgagttag gaaaatatta taaggattta
     4201 agaaaagttt ataataaatt tgaaagtgat ttaactaatt cttgtgcaga aatatataat
     4261 tttgaaatac caggaggcca atatacaaac ttaaaacctc aagctgatag tttaggatta
     4321 gttaatagat ttgatgaagt taaagaaaaa tataaagaag ctaatgaagt tgtaggagat
     4381 ataataaaag taactccatc ttcaaaagtt gtaggagact tagctatctt tatgactaaa
     4441 aataaattag ataaagacaa tataatagaa gaaggtaaaa atttatcatt cccagattct
     4501 gttgtggatt actgtaaagg tatgattggt caaccagaag gtggaatacc aaaagattta
     4561 caagaggtag ttctaaaagg tgaagaagct ataacagtaa gaccaggttc attattacca
     4621 gcagaagatt ttgatgaaat agctaaatat ctaaatgaaa agtatgatat aaatgctaat
     4681 ataagaaatg ttataagtta tgctttatat ccaaaagtat atgaagatta tatcaaacat
     4741 ctacaacatt ataatgatat atctaaactt gaaagtgatg tattcttcta tggattaaat
     4801 aaaaatgaag aatgtgaagt agaaatagaa gaaggtaagg tattaacaat aagattagtt
     4861 gaaattggtg aagttaagga aaatggattt agaactatag gatttgagtt aaatggaatg
     4921 gttagagaag tagaaataaa agataagaat ttctctggaa aaattaataa tgttgaaaaa
     4981 gctgatatga atgaccctct tcaaattgga gcaagcatac caggaaaagt tataaaaata
     5041 atggttaaag aagaagatga agtaaaagca aatcaaccat taatcgttat agaggctatg
     5101 aagatggaaa ctataatagt tgctaagact gatggagtaa ttaagtcaat caaagtaaaa
     5161 gaagatgata tggttgaaga taaacaatta ttaatgataa tgaagtaatt taattaaaat
     5221 aaattaaaaa gcactctgaa atgtttaata aaaaatgttt tagagtgttt ttatttattt
     5281 taattaaata tttgttaaaa aaaagaggac aattacctcc agatgtagaa ataaagctag
     5341 taaggaaaat gttatctaaa aattctaaat atagtaaata ttttccataa tatttcttag
     5401 gaaataacag caaagataaa atacataaat ttaaaaggtg ggtgcttata tgaaggttta
     5461 tgatagtaaa atgttgagaa atgtagcagt attaggacat agcggatgtg gaaaaacaaa
     5521 tctaatcgaa actatagctt acacagcaaa cactaataag atacctaaat taacagacaa
     5581 ggtaaatatg acttatagca tgggattaat acctatagag tataatgatt ataagtttaa
     5641 cttattggat accccaggat attttgactt tagcggagac gttgtgtcat ctcttagagc
     5701 aagtgatgca gcaataatag taatagatgc aacagcgcct attcaagttg gtacagagaa
     5761 atcattagaa ctaactgaga gtattcctaa gataatgttt ataaataaaa tagataatga
     5821 aaaggcaaga tacaaagatg caatagcaat gttaagagaa aaatacaata ataagatagt
     5881 tcctatgata agtcctatat acaaagataa aaactttgta aagttacata atgtttttga
     5941 aaacatagat gatttagaag gtgaatttaa ggaacaagct atgagtgtta aagaagcatt
     6001 aatggaactt atagcagaaa ctgatgacca aatattagat aaatatttta atggagaaga
     6061 actaactacg gaagagatac aaaaaggtat tattataggt atacaaagag gtgatattat
     6121 acctgtaata tgtggttcaa ctataaataa tataggcaca aaggaaatat tagatacaat
     6181 atcaagttat ttagaaccta tctttacaga ggaaagcaaa ccatttaggg gattagtatt
     6241 taaaactatg gttgacccat ttgttggaaa gatgtcatat ataaagatta ctgagggtgt
     6301 actttctaag gataaagatg tttttaatat aaataaaaat gtaaaagaaa aaatagctaa
     6361 tatatataca ttgagaaata gtgaattagt agaaatagaa aaagctaaag ctggtgatat
     6421 agtagttata actaaagtta attctctaaa aacaggagac actatatctg cagataaaga
     6481 tgcagaagct ttagaaaaga tagattttcc taaaccacaa atatattatg cagtaactcc
     6541 taagaataaa ggtgatgaag aaaaagtagc atctgtatta aataaactag tagaagagga
     6601 ccctacatta cattggtata gaaatacaga gacaaaacaa gcacttttag gaggtcaagg
     6661 agaacttcat ataaaaacaa ttaaaaataa aatgaaagat aagtttggtg ttgatgttga
     6721 attgaatgac cttaaagttc cttatagaga aactataaaa ggaacagccg atgtacaagg
     6781 aaaacataaa aagcaatcag gtggtcatgg tcaatatgga gacgtaaaaa taagatttga
     6841 aagatgtgaa tctgattttg aatttacaga agaaatattt ggtggttctg taccaaaaca
     6901 atatatacca gcagttgaaa aaggtctaaa agactctatg caaaaaggta tattagcagg
     6961 ttatcctgtt acaaacataa aagctacgct ttatgatggt tcataccatg atgtagactc
     7021 ttctgaaatg gcctttaaga tggcagcaag tgcagccttt aaaaaaggta tggaagaagc
     7081 gcacccaata ctactagaac ctataatgaa acttaagata actgttccag aagaatatat
     7141 gggagatgtc atgggtgata taaataagag aagaggaaaa atatttggaa tggaaccaga
     7201 tgacaaaggg aaacaaataa tatttgcaga agctccacaa gcagagacat ttaaatatgc
     7261 aatagattta agagcaatga cacaaggtag aggatacttt gaaatggaat tagagagata
     7321 tggagaggtt ccatcacagt ttgcagaaaa aataattggg ttagctactg caaagtaatg
     7381 aacaaaataa atagaaaaat aattaatata tattattaaa aatattttta gggttttaac
     7441 taggtatagg tttatgttag gtaaacttat acctagtttt tatacatttt tgatgaataa
     7501 aaaaatatat aatactttct taaatgtttt aataaaaaga aagtattttt ttattattat
     7561 ttttacaaat gtttagtaat aaagatgaga aggtatattt aaaatataaa gtatttaaag
     7621 ttctgaaatc taatcatgaa gcaaaaaatt agcatttggt tatcaataaa tataataaat
     7681 aaaaactcat aattaatgaa agaataaatt catatctaaa tttctataaa aaatttaaat
     7741 ttatacaatt tagggcttta aaaatttaaa taataggtgt aaaataatag tcaaagaaag
     7801 tcaaaaataa aaaggtgtgt ggtgatatta atggcaacta tgacagatat aattgaaaaa
     7861 tttattaaag atttgatgga agaagataat agcatacaaa ttcaaagaaa tgaactagca
     7921 aacttgttta gttgtgcacc atcacaaata aattatgtct taacaacaag atttacaata
     7981 gatagaggat attacattga aagtaaaaaa ggtggcggag gatatgttca aatagaaaaa
     8041 atacgaaaaa gtaaagatgg acatattaga gaactactaa atgaaaaaat aggaagtcag
     8101 atatcctata aaaaagcaaa ggaattatta gaaggtttaa aagagtcgga tttgattaac
     8161 gaaagagaat taaaattaat attatatgca atagatgata aatcattgtg tatgccaata
     8221 tatgaattaa aagagaaggt aaggtctaat attttaaaga atataataat aggattattt
     8281 agtatagagg agtgataatt tatgctatgt caaaaatgca ataagaataa agcatctgta
     8341 tattataata agatagtcaa tggagaaaaa acagagatgt atttatgtag tgagtgtgca
     8401 aaagagaata cagaaatgaa ttttaactta gatatgcctt tttcaatgat ggatatattt
     8461 tctaatttag gttttcaacc taaaaaagaa cttgaggaaa agctagtttg tcctaaatgc
     8521 aatacaacat atagtgagtt taagaataat gggagatttg gatgtagtga atgttataat
     8581 gcatttagta gtcaagtaaa tccaatgctt caaaatatac atgggcatat agaacataca
     8641 ggaaaagcac ctaaaaaatc tttttataag ataagtgtag aaaatgagat taaagaacta
     8701 aaagaagatt tagatagagc tataaaaaat gaagagtatg aattagcagc tcaatttaga
     8761 gataaaatta aatatttaaa aggttctatt gactaagatt ggagtgatat tatgaaagag
     8821 aacatagtca tgaaatcaag agttaggcta gctagaaatt taaataacta tccatttcca
     8881 aataaattag ataaagaatg tgccatggaa ataatagaaa aagtaaaaaa tgcgtttatt
     8941 aattctaact tagaacaaaa agaagaattt gatttctata aaattgaaga tttagaccaa
     9001 tctaaaaaaa tgcttatggt tgaagaacat ataataagcc cagatttagc agaaaatgat
     9061 aaaagtgcag ttatagtaaa aaaagataaa actataagca taatgataaa tgaagaagac
     9121 catataagga tacaaactat atgtgatgat ttaaacttag aatatgcata tagtgtagca
     9181 aatgaaatag atgatttgtt agaatcaagt ttagaatatg cctttaacac aaagctaggt
     9241 tatctaacat cttgtccaac taatacagga acaggaatga gagcttctgt aatgatgcat
     9301 ttaccagcat taagtcaact aggctatatg gatgaacttt ataaaatttc atcacaaata
     9361 ggtatagcaa ttagagggat atatggagaa cgaacagaag cattgggaaa tatatatcaa
     9421 atatctaatc aacttacact tggaagaaca gagtctaata ttatagaaaa tgtaagtgga
     9481 cttacaaaag atgcaatttc aaaagaaata aaagctagag aaatattgca aaaaaaatta
     9541 ggtataaaac tagaagataa aatatttaga tcgataggga cacttgaaaa ttcaagagta
     9601 atgagtagcg cagaggcaat gagccatcta tcaaatataa aaatgggaat agaaatgaac
     9661 tatatagata aactagattt aagggctata gaacaactta tgataggtat acaaccagcg
     9721 catcagtcaa taatgtataa gagtgatgat gttgagaata gagatataaa tagagcaaca
     9781 tatattagag aaactttgga aaaattaaga ggagggatga attgtgaact tcaatagatt
     9841 tacacaaaga gctaaaaagg caattgattt agcgtttgaa tctgctaaaa gtctaggaca
     9901 caatattgtt ggaagtgaac atatactttt aggtctttta agagaagaag aaggtatagc
     9961 tgctaaagtt ctaagtaaag ttggatttac agaagcttat ttagaaggca aaatagttga
    10021 tatggaaggt aagggagaag aaatctcaga agatatagta ttaagtccaa ggagtaaaca
    10081 aatacttgaa ttatcaggaa tgtttgcaaa taaattaaaa acaaattata ttgggactga
    10141 gcatatatta ttagcaatta ttcaagaagg tgaaggaata gctaataaga ttttaaatta
    10201 tgctggagta aatgatagga ctttagccca attaacaatt gatatgatgg gtattagtga
    10261 taaaaatcaa tacaaagcag agaatagtta cacgggcaac cagaatcaag cagaatctaa
    10321 agtacttgat aagtatggaa gaaatcttac actatatgca aaacagaata aaatagaccc
    10381 tgtaataggt agagaaaaag aaatacaaag agtaatacaa atattaagta gaagaactaa
    10441 aaacaatcca gtgttgatag gagacccagg tgtagggaaa acagccatag ctgaaggtct
    10501 ggctacaaat atagccttag gaaatgttcc agaaacacta aaaaacaaaa ctttatactc
    10561 tttagagatg ggttcattat tggctggggc taagtataga ggagaatttg aagaaagaat
    10621 taaagaagtt gtagatgaag ttgttaaaaa tggaaacata atcttattta tagatgagat
    10681 gcacacaata ataggggctg ggtctactgg agaaggctct atagatgctt caaatatatt
    10741 aaagccagcc ttggcaagag gagaaataca agttataggt gcaactacaa ttgatgaata
    10801 tagaaagcat gttgaaaaag attctgctct agaaagaaga ttccaaccag ttatggtaga
    10861 tgaaccaact aaagaggact caataaaaat attagaagga ttaagagata aatatgaagc
    10921 tcatcataaa gttaaaataa ctgatgatgc tataaaggca gctgtagaat tatcaactag
    10981 atatatatca gacagatatc tgccagataa agctatagac ttgattgatg aagctgcatc
    11041 aaaagtaaga ttaaaagaaa atacacctcc ttcagaaata aaaaaattag aactagaaat
    11101 agaaaatata gataaagaaa aagaagaagc agtaagatgt caagactttg agaaagcagc
    11161 caaaataaga gatgaacaag gtctacttaa aaaacaatta gaagatgtta gagaaagatg
    11221 gaacaagtca tctaaacatt cagatttagt tgatggagaa gttattgctg aagtagtggg
    11281 cttatggaca ggaataccag ttaataaaat tcttgaagaa gaggctgata gacttttgag
    11341 acttgaggaa atactgcaca atagagttat aggtcaagaa caagcagtaa aatctatttc
    11401 aaaggcaatc agaaggtcaa gagcaggtct taaagaccct aatagaccaa ttggttcatt
    11461 tttattttta ggtccaacag gagtaggtaa aacagaatta tctaaggcat tagcagaagt
    11521 gcaatttgga gatgaaaatc aaataattag aatagatatg tctgaatata tggaaaaaca
    11581 tgctgtgtca agaatgatag gttcacctcc aggttatgta ggtcatgatg aaggtggtca
    11641 attaactgaa aaagtaagaa gaaatccata ttcagttatc ttatttgatg agatagaaaa
    11701 agctcatcca gatgtattta atattctgtt acaaattcta gatgatggta gactaactga
    11761 ttcaaaagga agaactgtag attttaagaa tactattgta ataatgacat caaatgttgg
    11821 agcatctaca attggtagac aaaaaacttt aggatttagt atagctaaag gagacgaaga
    11881 agaaaaatct caatatgaaa aaatgaaaga aaatataatg ggtgaattaa aacaaagatt
    11941 cagaccagag tttttaaaca gaatagatga tataattgtt ttccactcac taaatgaaaa
    12001 tcatatatca aaaatagtac ttttaatggc agcgaaacta caagagagat taaaagaaat
    12061 ggatataaaa ttagaaatga gtgatgaggc tgttaaatta atttctaaat ctggatttga
    12121 cttagaatat ggagcaagac ctcttaaaag agctttacaa aaagagttgg aagatgagct
    12181 atctgaagca atcttaaaag gtaatgtgaa aaagggaagt aatgtagtag caaaagtcaa
    12241 agatgaaaaa atagtttttg aaactaaata atttaataga gttgcatttt aaaatattgc
    12301 aaatataagt tttataatct aaacaaaatt ttaagggcaa tgaatctata ataattcatt
    12361 gtccttaatt attttattta atctttacat attgtctttc agatatgcta aaattatagt
    12421 atagaataaa aaattaagga tgataaaagt atggcaaaaa taaaaacaaa atatgtatgt
    12481 caatcttgtg gatatgaaac agctaaatgg cttggtaaat gtccagaatg tacaaaatgg
    12541 aatacatttg tagaagaaat agaccaaaaa agcaccaaaa aagaggtctt tataatagat
    12601 aaatcttcat ctaaaccagt cagtataaac tcaatagaaa gtaaagaaga agagaggttt
    12661 acaacagata taaatgaatt agatagagtg cttggaggag gtatagttaa aggctcttta
    12721 gtgcttgttg gtggtgaccc tggtataggt aaatcaacac ttttaatcca agtatctagt
    12781 aatgttgcca atttaggaaa aactgtttta tatataactg gagaagaatc agaatctcaa
    12841 ataaaaatga gagctaaaag attaggtatt aactcagaaa atttatatat atttgctgaa
    12901 aataatttaa gtataattga atcttattta gaaagtgtaa atccagaatt aataattcta
    12961 gactcaattc aaactgtctt cagtccagaa atatcctcgg caccaggaac tgtcagccaa
    13021 attaaagaag gtacttcaaa attcatgaaa atttctaaaa aaatgggaat ttcaacgttc
    13081 atagttgggc atgttacaaa agaaggttca ctagcaggac caaaattatt agagcatatg
    13141 gttgatactg ttctatattt tgaaggagaa agatataata cttatagatt agtaagagct
    13201 gttaaaaatc gatttggttc aactaatgaa cttggagtat ttgaaatgag ggatttagga
    13261 ttagtagaac ttgataatcc atctaaaatt ttaatctctg aaaaaccaaa agatgtagca
    13321 ggttcagtaa taatatctac tgtagaaggt acgagaccaa tgttacttga attgcaagca
    13381 ttagtttctc ctacaagttt tggtatacca aaaagaacat ctacaggagt cgattacaat
    13441 agagtaggta tgttattagc agtattagaa aaacgtgttg gtttgcaaat acaaaatcaa
    13501 gatgtctata taaacatagt tggaggaata aagataaatg aaccctcaat tgatttaggg
    13561 atagccattt ctgtagcatc tagttttaga aatataccga tagatgaaaa tatagctgtt
    13621 acaggagaag tgggacttac aggagaagtt agagcagtaa gctttataga aaagagaata
    13681 gctgagtgta aaaaacttgg atttaaaaag atagtagttc ctagaagtaa ttatgatgtt
    13741 gtaaaagaga ccaaaggcat agaaatatgg ccagtagata acttaagaca agctataaat
    13801 atagtacttg ggaggaatca ataaatatgg agaattttct agataataaa aatatgctat
    13861 atgcattaaa aatgatatct cctggaactc cacttagatt aggtctaaac aatgtactaa
    13921 gagctaagac tggtggatta attgtaattg caacaaacga agatgtaatg aaaatagtag
    13981 atggaggatt tgctataaat gcagaatatt caccatcata tctatatgaa ttagctaaaa
    14041 tggatggagc tatagtttta agtggtgatg taaagaaaat attatttgct aatgcacaac
    14101 ttatacctga ctattttata gaaacatcag agacaggaac aagacataga acagcagaaa
    14161 gagtagcaaa acaaactggt gctatagtca taggaatttc acaaagaaga aatgttataa
    14221 cagtttatag aggaaatgag aagtatgtag tcgaagatat atctaagata tttactaagg
    14281 caaatcaggc tatacaaact ctggaaaaat ataagacagt attggaccaa gctgtaacaa
    14341 atttaaatgc cttagagttt aatgatttgg taactattta tgatgttgca ttagtcatgc
    14401 aaaagatgga aatggtaatg agagttacaa gtataattga aaaatatgtg atagaattgg
    14461 gtgatgaagg aactttagta agtatgcaat tagaagaatt aatgggtaca accagaatag
    14521 accagaaatt aatattcaaa gattataata aagaaaacac agaaataaaa gaacttatga
    14581 aaaaggtcaa aaatttaaat tcagaagaac taatagaatt ggtcaatatg gcaaaactat
    14641 tagggtatag tggtttttca gaaagtatgg atatgcctat aaaaacaaga ggttatagga
    14701 ttcttagcaa aatacataga ctaccaacag caataataga aaacttagta aattattttg
    14761 aaaactttca acaaatttta gatgcatcta ttgaagaatt agatgaggtt gaaggaatag
    14821 gtgaaataag agcaacatat ataaaaaatg gactcataaa aatgaaacaa ttagtcttat
    14881 tagatagaca catatgatga attttaaatg aattttacta cttttttctt gaatataata
    14941 tacactttgg tctataatag ataagtataa ctagggggtg aagcattgat aaggaaagta
    15001 acaagaatat tgtttatttt acttggattt acaataggta taacaaccta tttaacatta
    15061 atgaaagatt ttgaaatatt aacatttgga aaagaaacat atggttatat cgcagctgta
    15121 gtagcaggta taataatagc cattttagga tatcttatag aaccatgggt tgtaaataaa
    15181 gtaaaagaaa ttgctaaaat agtggataaa gaattgtcaa aatatcctca gactgatata
    15241 ttgttagggt ctatgggtct tatagtagga tttgttattg cgtatttatt aagtggatta
    15301 gtaaatagaa taccaatagt aggtggaata ctatccttac tattatattt attcctagga
    15361 tatcttggaa tgaaagttgc gcttaaaagt aaaaatgatt tatttaatat tggaaaacta
    15421 ggaagattag caaatcctat taaggataaa gataaggata aagaaaataa aaaagaagta
    15481 aaagccattc ctccaaaagt attagataca agtgtaataa ttgatggaag aatagctgat
    15541 atttgtaaga ctgggtttat agaaggaaaa cttattattc ctgcttttgt tttggaggaa
    15601 ctaagacata ttgcagattc ttcagatgat ttaaaaagag taagaggtag aagaggtctg
    15661 gatatattaa atataattca gaaagaatta aatatagaag ttgaaataag tgagagagac
    15721 tttgatgata tagcagaagt tgatagtaaa ttattaaagc ttgctcaggt tttaaatgga
    15781 aaagttgtta caaatgatta taacttaaat aaagttgctc aattccaagg tgttgaagta
    15841 ttaaatataa atgagttagc taatgctata aaaccagttg ctataccagg tgaagatatg
    15901 gttgttcaag tggttaaaga aggaaaagag gctcaacaag gtgttgcata tctagatgat
    15961 ggaactatga tagttgttga tggaggaaga aaatatatga atgagactat aaaagtactc
    16021 gttacttctg ttcttcaaac tcctgcaggt agaatgatat ttgcaaaacc taaaaattaa
    16081 tatgttcaaa gaaacctata atttgggctg tttattttaa atagatggtc caaatttatt
    16141 tatttataac gataatgttt aatgtattct taaagatggg gatttaaatc agaaagcagg
    16201 gagaatttat gagaaaaaag attatatctt tcttagcaac atttatcata atattaacat
    16261 cagcaagtca gtatagtttt gcagatgata tgtcaacacg tggaaaagta atatttatag
    16321 atatgaatag aactagtatg agtaatatgt taagaataaa atcattaaga gaagaactag
    16381 acaatagagg gtatattggc ttgatgaata taagaggcga taaaggaagt gatgatagaa
    16441 ggtcatatgc tagtatgggt gctggtggta gagctaatgt ggctaatgaa gaagatatta
    16501 attttgaaag ctcgagtaag gatagaaata tagtttttga atcagctaca ggaaaaagtg
    16561 caaaaggtat aaataattta actataaata agtctataaa tgaaaattta aactttggag
    16621 agtatggttc tgtattaggg tcattaggac aaagtttatc tgagaatgga ttaaaagcat
    16681 ctgtattggg taattcagat ataattgaaa atggtcaact tataaaaaat agaaacttat
    16741 gtctaactgc aatggatgaa tatggaagaa tacctaatgg aaatgtagat actataaata
    16801 aaaaagactt atctatgcct tatggaatat caactgatta tgataaattg attgtagaga
    16861 ccaaagaagc ttacaaaaat aatgatgtgg tttttgtgga gcttggagac acatatagat
    16921 tagatttata taaaccaaat ttaaatgaaa aaacatatga gagtgtgaaa gataacatag
    16981 aaaaaaatat agatgtatat ttaaaaaata tattttctat ggttgaagaa aatgacactg
    17041 tctacatagc aagtgcattt ccaagtgatt tagattataa aaataaaaga agactttctc
    17101 caataataaa attaaatgga gaagggaaag gtatattatc atcttcaact actagaagag
    17161 aaggtattgt aacaaattta gatgttgggg ttgaaatatt agacaacttt aatataaaaa
    17221 atcaaaatat ggttggaaga aaatatgaac ttattaacag agatgataat aaagaatttt
    17281 tgatggatga atatcaaaag atagtatcta tttcaagcat aaggtctaca atactgaatg
    17341 gttttgtaag tatagttttc ttgtcatgga tagttgctat gattgcaatt ttatttagaa
    17401 agcatatatc aaaaaattat aaagaaacaa cattttttat attaaaggaa cttttaaaaa
    17461 taggaatagt aatgccactg tcatttatga taacaccaat aatgaatttc aaaacaccat
    17521 tagcaatatc tttaggaata attataataa cacttactat atatcttata agtaaagttt
    17581 taataaagaa tgatttaaaa aacatgctgt tttttactgg attaaccata gtaattatgg
    17641 ttattgatgc tgggtttggc tcatatttaa tgaaaagcaa tgttatgagt tatgactgta
    17701 taattggagc tagatattat ggagtgggaa atgagtatca aggtgtggca atcggtagtg
    17761 caatatttac atttgcaatt ttacttacat ataaaaatat accaaagtgg tctataatag
    17821 ttttttcttt ggttatctta attacaagtg catctccaat tatgggagca aatgttggaa
    17881 gtgctatttc tgaatgtgtt gcatttttac tatttatact attgatatat aatgtgaaga
    17941 tagacttcaa aaaaattgta ttattaggta tagcagtatt atttgtatta ggagtttttg
    18001 tagcaataga tatgattttg ggaagtaatt cacatttagg tatgtttgta aaagaaattt
    18061 actttaatgg accaggagaa ataatacaaa catttagtag aaagatagag atgaatttaa
    18121 agctagctca aaccagtgca tgggtaaata tactgttaac tggaataggt gtaattttag
    18181 ttcttatgat taatcagatt agatacttta agcaactgat ggatgagtat ccaattgtat
    18241 ttaaaggatt tatagcatca atagcaggat gtttagtaac tcttttagtg aatgactctg
    18301 ggattgtgtc atcagctaca gcctttattt atgtaattgt acctatgata acattaagta
    18361 taaatctcac agcattaaaa gaataagtac tgtatgaata atatgtaaat atgaacacat
    18421 taatttaact tctttattta caaaataaag aagaaagttt ataaaaaata aattataata
    18481 aaaaagagtt tacctcaaaa ttgattattc taatcaaatt ttaaggtaaa ctttttttat
    18541 tataaagata aataacatat aacctcatat tttaggtaga tttttttctt atatgtttag
    18601 gagtgcattt aaatttttcc ttaaaaattc tgtaaaataa ttttgtatta gtaaatccat
    18661 gagcttctaa tatactgcat aaggtatcat ttgtgttaat taagtcttta taaatatgag
    18721 aaatacgcac atcatttaaa tactctaagt aagtttgtcc catatactgt ttaaattttc
    18781 tacaaaaata ttctggctgt aagtgtgcta ccttagcgat tttgttaata gaaataggca
    18841 ttttgtaatt aatctttgta tattctaaaa ctggttcaaa ttttgacaaa tcaagtactg
    18901 tttttttcat atttttatta cctacagata ttttaaagtt tgtatataat tcaaataaaa
    18961 gttcaaataa taagcttgta aagagcagat tagctgcagg aggagagcta agtttaattt
    19021 ctctcatgtt ttctaatata agtttaactt tacctgaatt ttttttatag ttattgtcat
    19081 tggcattaac cttaaagtca aaatagcaat ttttaaaatc agggatatat ttgtttagaa
    19141 aagacaatgg tacttgcaat aatatagagg tatcttgata agtacatcta gttgagtgaa
    19201 taattccaga attaatgaac atacactcat tagcttttaa attataagtg taaccctcca
    19261 tgttaatttg taaatcacca gaagttatat aaattatttc taatgagtta tgccaatgat
    19321 ttgctacata actaccacta tcatcaaata gtttgaaatt aacatctaaa ttatcaatag
    19381 gttcaattat ttcatgtgaa aaattatttc tgttcataat aatatcctcc ttataatttt
    19441 aaattataaa tagtaaattt ataatgttca atattaaaaa gataatatag actaataaat
    19501 tagataataa acatacttaa gaaaacggat aaaatcaaat ataataactg tatattaaaa
    19561 taacttaaaa aaattatttt attataagtg caaaatatac ctatttaaat aagataagag
    19621 gggaggattt tatgtgtata taaaaggtgt aaatttagga ggttggttag ttttagaaaa
    19681 atggatgacc tcaagtttat ttgaaggcac agaagctgaa gatgaatact atttaccaag
    19741 acagctttca agagaggcat atgaatctag aattaagact catcgtagtg aatacatcac
    19801 agagagagat tttgcaacga ttaaatctat gggatttaat tcagttcgta taccagtacc
    19861 atactttatt tttggagatt gtgaaccatt cataggatgt gttaaagaac ttgataaagc
    19921 atttgcttgg gcagataagt atgggcttag tatattgata gacttacata cagttccagg
    19981 aagccaaaat ggatttgata atggaggaat atcaggtata tgtagctggt cacaaaatcc
    20041 agaatatgta gcgtttacat taaatgtatt agaaagactt gcaaaacgtt atgggatgag
    20101 acatgaattg tatggaatcc aaattttaaa tgagcctata acagaaagaa tgtggaacat
    20161 aatgaatgtt ccaaatcggt ttaaggctgt agataaggag atggcaaggg ggagtaaacc
    20221 taattcatta gagtttttac gtgattttta tataaaagca tataaggtaa tgcgtcctta
    20281 tatgagagag gaaaacgtta tagtgtttca tgatgcattt gaattaaaag catggaaaga
    20341 ctttatgaga gaggaagaat ttaaaaatat tgttctagat acacaccaat atttgatgtt
    20401 agctgaatca gatggatgtg aacaaagtat tgatagctat ctcaaatata ttagagaaaa
    20461 ttatgctaaa gatattttac aaatgcaaaa atattttcct gttatatgtg gggagtggag
    20521 cttatttaat tcatatgctt gtggtataga tacaaatgga ggtcaatcac ctctaaatgg
    20581 gattgaatca aatatagata aattatcaaa agacgataaa agagaattat ataggaagat
    20641 agcaaaagca cagcttgatg catggagaaa tggaagtggt cactactatt ggaattataa
    20701 gttattacta gatacagtta atgaagaagg ttggatagga tgggatagtt gggacctagg
    20761 caaatgtgta gctcaagaat ggtatcctat agaatattaa actaaaattg gaggaaaaga
    20821 tatgattaaa aaccctatac ttccaggttt taatccagat ccttgtattt gtagaaaggg
    20881 agatgattat tatcttgtag tttcatcttt tgaatggttt ccaggtatac ctgtttatca
    20941 ctctaaagac ttaaaaaatt gggagttata tactcatatt ttgacagatg aaacaaaaat
    21001 agatttgaag aagcttcctt catctaaagg aatttgggca ccttgtttga cttattgtga
    21061 agaagaagat ttattttaca tagtttatgg aattatgaac tctatgaatg caagatattt
    21121 tgatgttgat aattatttaa ttacatcaaa agatataaag ggagagtgga gtgaaccagt
    21181 atatatacac tcatcaggtt ttgatgcgtc tattcttcat gatgatgatg gtaaaaagtg
    21241 gattgcatca ttagactggg agactagaga aggatatgaa aaacctggtg ttatatgttt
    21301 agtggaatac tgtacaaaaa agaaagaaat agtaggatat ccaaagagaa tatggtcagg
    21361 gggaactgat agaggttgta ttgaagcacc acatataaca aagcgtggag attactatta
    21421 tataatgtgt gcagaaggtg gaactggata tggtcatagt gtaactatgg gaagggctaa
    21481 aaatatatgg agaccttatg aaaaagattc aatgaatcca attgtaactt caattccagg
    21541 cgatttttat gaaagacatg accctgacca tctaaagcct aaatactata atccagaatc
    21601 aaaacttcaa aaatctggtc atggaagcta tatagaaaca acatcaggtg aagtatattt
    21661 agttcatcta acatctagac catttgtacc agagttaagg tgtactttag gtagagaaac
    21721 agctattcaa aaaatgaaat ggacaaagga taattggctt cgaatggaag atgaaagtaa
    21781 tttggcaaaa gaatacgtat cagaaagtaa actagaagaa catttagttt catctatacc
    21841 aagttttgat gactttgatt caaatgtatt aggtttacag tactatgctc ctaggatttc
    21901 accattatca tttgcagatg ttaagtctag accaggatat gtaaggatta gaggtcaaga
    21961 atcaagaaca tcattaaata aagtaagcat attggcaaga aaattaactt cagtatatgc
    22021 aagaataacc actaagatgg agttttaccc agaggtacat cagcatagtg cgggtttgat
    22081 aatgtattat gataatatga attatataaa tttaagaaag tattatagtg aaacattggg
    22141 tcaaagtgca ctatcaataa tacatcttga aaatggagag aagacggaat ttttaaatac
    22201 taggatacca ataaaagata taccaatata tcttcgtctg tatatacaag gtcgtaagtc
    22261 atattttgaa tggagttatg atgaaaaaaa ctaccaaaga ataggaaagg tatttgatac
    22321 aactaagttt tcagatgagt actgtaaata tggagaattt acaggtacgt ttataggatt
    22381 aacttgtgca gatcgtgtaa agcataaaca ctatgctgat tttgattttt ttgaatatat
    22441 agcagatgaa tcaaaagatg tcgattaata aaatcattac taaaattttc agagcaaagg
    22501 taaatcttat gctctgaaat aagaaacatt agaaggcaaa tcttacaaaa aggagtatgg
    22561 tattatgaat acagaagtta atgttaaagc aactacgatt agatatgaaa agatttcatt
    22621 ttttgaaaga atttcttatg gttgtgggga tttggggtgc aatatcatat actcagctat
    22681 gtcagcattt ttgctgtttt attatactaa ttatgcagat gttagtgcag cagctgtagg
    22741 tagtattatg cttgtatcta ggatactgga tgggtttagt gacttaacaa tgggaattat
    22801 agtggataga actaaatcca aatacggtaa ggctaggcct tggattttga gaatggcgat
    22861 tccttttgct atagcagcag tattattatt ttctgttcca tcaaatcttg gtatcacttc
    22921 taaattaatt tatattttta ttacatataa cttagtttct actgttattt atacagcaat
    22981 caatgtaccc tatgcaactc taaattcact tataactcaa aaccagtatg aaagaggagt
    23041 attaagtata tttagaatga tattagccac ttgtggtacc ttactaataa atggacttac
    23101 actacctcta gttgaatact ttggaaataa tttatcagca tggactaaaa cattctttgt
    23161 atttggaata gcatctataa tggtattttt tataacattt actggaacca aggaaagagt
    23221 aaaagcagtt aagcaagata ggaatgaagt aataccattt aaaataggta ttaaatcttt
    23281 atttagaaat aagtactgga tacaaataac attatgttta gtttgcatat ttattgtatt
    23341 tgcgataaat ggtggttcat ccgtatatta tgcaaagttt attttgggag atgaaaaatt
    23401 atttggtcct ataaatatgg tttcaaatat atcccaaatt atagctatgt ttatggttgc
    23461 tccttttata aaaaaatttg gaaaaagaaa tgtattaata gtaggttcaa taatattaat
    23521 atcgtcaaat gttatgttta taatagttgg acaaaactat ataggagtaa tatttgcaag
    23581 tgctataaag ggaataggaa gtgctggaat agcatcaaca atgtttgcta ttgtttcaga
    23641 tactattgaa tatggagaat ggaagactgg atatagaact gaggggctta taaatagtgc
    23701 atctagtttt gggtttaaag tgggaaatgg attaggttca gcaatattag gtgcagtatt
    23761 atcaattggt ggttatgtag gaacatcagc tactcaaagt gatttagcta tagtatcaat
    23821 aaaagtatgt tttatatacc tacctatatt tataacaata ttacaaatta tcataatgtt
    23881 tttttataag cttgataaag aatatagtac tatacttaat gaattaaata ctaaataatg
    23941 aaaaagtttc ttataaagtc ttaagtttaa ttaaggtcta ttgtaaatgt tccctataaa
    24001 aactattata tatttatgat tattgaagaa tttttagaga agcgaataat aaaaatataa
    24061 gaagacttta aatgatgaaa tggttggtct tttagaaatg atttaataag ttatttttat
    24121 ttaaagcatt ataagtggac aattaatttt taatttatgg agatgatatt taacaattag
    24181 aagtaatata taatatgcgc taagaagcaa tttttcaact atttatgaag gtggtggtac
    24241 aagaaaagta tcaccttttt tttgttatac aaaaaaacag ttgacttttt atatgaaccc
    24301 cttctataat gttactgaaa ttaagtaaaa tgattgaaca aatgtaaaga taattgaaaa
    24361 gcttgatttt ggaggaaatc aaatgaaaaa aattggagac ttaagattaa tgatgaagtg
    24421 ttgttcactt tattacgaag ataatttgaa tcaacaggaa atagcaaatc aattgggaat
    24481 atcaagacca acaatatcaa gaatattaaa agaagctttt gaacaaggaa tagtaaaaat
    24541 acagatagtt gatgttttga aaaatgatta ccaaaaaata gagagaagtt tggagagaaa
    24601 atataaatta aaagaagtaa tagtagtaga tgataaacaa gatgcactaa cacaaaaaca
    24661 agaactagca agagctgtat ctgaatattt aactagagta gtaaaggaaa atgatataat
    24721 aggtgtttca ataggaacaa cactaaaaga aataccaaga tatgtggaaa aaagtaactg
    24781 taaaaatgtt acatttatac ctctattagg agggattgga gacaatgaaa tagatattca
    24841 tgcaaatcaa atagcagtaa gtctagcgag ggcttttgga ggtgatttta aattattaca
    24901 tgctccagct gttatgtctg acttgtctac aaaagagaaa ctttgcaaag acgaaaaaat
    24961 taaagaagta ctagatttaa tagataaaac aactatagca attgtgggta taggaaatcc
    25021 tatgagttta aactcaacaa taatggcttc tggatacatg aatgaagcag atatagaaga
    25081 tttaaaaaaa tacaactcaa taggtgcaat ttgtttacaa gcctttgata aagaaggaaa
    25141 aacttctata cttgaattta atcaaagagt attaggagtg aaactggaag atttaaaaaa
    25201 gataaaaaga acaataggtg tcgcttcagg agatgaaaaa atagaggcaa taaaggcatc
    25261 attaaaagcg aaatttataa atagtttagc aattaatcac agtttagcac ttaaattatt
    25321 agaagattgt gactaataga aagatagata aaagttaaca ggagggaatg tatatgtcaa
    25381 tatcaaaaga aacattgctt gaaatgtata agagaatgaa tcaggctaga aaatttgaag
    25441 agaaagttag ttggtttttt gcaagaggta tggttcatgg aacaactcac ttatctgtag
    25501 gtcaagaagc gtcaagtgtg gcggctgtaa tggctttaga aaaaggagat ttagtatcat
    25561 taactcatag aggacatagt caatttatag gaatgggaat agacctaaat aaaatgatgg
    25621 ctgaacttat gggtaaagaa actggatttt gtaagggaaa aggaggctca atgcatatag
    25681 cagatattga gtcaggtaac ttaggagcaa atggagttgt aggtggaggt cttacaatag
    25741 ctccaggagc tgcacttaca caacagtata aaaaaacagg caaaatagtt ttatgtagtt
    25801 ttggtgatgg agcatcaaat gaaggtactt ttcatgaagg aataaattta tcttcaatat
    25861 ggaaattgcc aataatattt tactgtgaga ataatttata cggtatgtca acaagcataa
    25921 aacgtcatat gaatatagaa agtattgcca ctagagcagc ttcttatgga atagaaggaa
    25981 tatcaataga tggatataac ccaatagaag tatacgaaac agtacaaaaa gctgctgaga
    26041 agtgtagaag aggagaagga cctgtattaa ttgaaagtag aacttataga tggttaggtc
    26101 attcaaaatc agatgcaaat gtatatagaa caaaagaaga aatagaatca tggaaagcaa
    26161 aagaccctat tgaattttta aaaaattatc ttatagaaaa taatctatct aatgaagatg
    26221 aattagataa gattcaagaa tttgcaaagc aatcaataga agatgcagta gagtttgctc
    26281 aaaatagccc aaatccaaaa atagagtctt tattagagga tgtatatgca gactaagaat
    26341 ggtaaaggag gaaaataaaa atgagtacaa gggaattaac atatgcacaa gcaataaagg
    26401 aagcaatgtc agaagaaatg agaagagatg aaaatgtaat atttatggga gaagatatag
    26461 gaatatatgg aggggcattt ggtgtatcag ttggtatgat agatgaattt ggaccagaaa
    26521 gagtaaggga tactcctata tcagaagcag ctatagcagg agcggcagca ggtgcagcag
    26581 caactggttt aagacctatt atggaagtaa tgtttatgga ctttgtaaca atttctatgg
    26641 atgcaattgt aaatcaagcg gcaaaaatga gatatatgtt tggaggaaaa gcacaagttc
    26701 caatggttgt tagatgtcca ggtggttctg gaactggttc agcagagcaa cattcacaaa
    26761 gtttagaagc ttggttttgt catgtaccag gagtaaaagt agttgcacct tctacaccag
    26821 cagatgctaa gggattgttg aaagcagcta taagagataa taatccagtc atatttgttg
    26881 agaataaatt gttatacaga aaaaaaggtt ttgtgccaga agatgattat gtaattgaaa
    26941 ttggaaaggc agatattaaa agagagggta ctgatgttac agtaattact tatggaagaa
    27001 tgttacaaag tgttgaagaa gcagcagaga ctctatcaaa agaaaatata aatgttgaaa
    27061 ttatagattt aagaacttta tatccattag ataaagagac tatagtaaag agtgtatgta
    27121 aaactgggag agttttaatt tgccatgaag cagctaagac tggtgggcta ggtggagaaa
    27181 tatcagccct gataacagaa agtgaatcat ttgattattt agatgctcca gtaaaaagaa
    27241 tttgtggaaa agatgtgcca ataccatata atccagagtt agaaaaagca gttgtcccaa
    27301 gagttgatga aatagaagaa gcaataaaat ctttaattgt tagataagga gggatagata
    27361 tggtggcaaa taaaataaaa gccacaccag cagctagaag tcaggcaaga aaagacaata
    27421 taaaattaga taggctgatt ggaagtggag aaaatggaag gattcattta gtagatgtgt
    27481 taaattattt aaaagataat aaagcaaata ctactccttt agctagaaga atagcagaag
    27541 atttaaatat agatttagaa actattgtag gtactggata tagtggaaag ataagaaagt
    27601 gtgatgtgga aaaattaact gcaaaagaaa ctattgtatc aaataataca tctaaatata
    27661 gtgagaaaaa agagctgaaa attgaaaatg aaaattcaag catgtttaac acagtagaag
    27721 gaatatttga aaagccaaat ccaatgagag ctactgtagc aaaacgaatg tcagaaagtt
    27781 attttagtgc tcctgtattt acattcaata tagaagtaga tgctacagaa ttgaaggtac
    27841 ttagagcaaa gttaatcgac acagtaaaag aatcaactgg agtaaaatta actatgacag
    27901 atttaatagt aatggcagta tctaaaatac ttccaaatca tcaagcctta aattcagcat
    27961 ggacagatga gggtatattt agatataaag atgtaaatat agctattgca gttggtttag
    28021 atgaaggctt atatgttccg gtagtaaaaa atgcaaacaa aaagtcttta aaagaaatag
    28081 caaaagaaag caaagaactt gcggaaaaag taaagacagg gaaacttatg ccagcagacc
    28141 aagaaggtaa tacatttaca ataagtaatg ttggaatgta tggtataact acatttacac
    28201 ctataataaa tatgccttca agtgcaatct taggggtagg tgctacacaa gataagtttg
    28261 ttccagtaaa tggagaggct aaaataaaac ctataatgaa cttatcatta acatcagacc
    28321 atagagtaat agatggaaca gtagctgcaa aattcttaaa agatttaaaa gaattgttag
    28381 aaaatccttt atcaatgctt gtataggttt tgatatttcg ttactgatat aaatagtttg
    28441 aatagattat attattataa ttgtttcagt aaatttaaag cttttatata ttaagaatta
    28501 tttttaacag taactatatg tgaatatgat ttgcttaatt aaaatatatg aaaataaata
    28561 ggaggagagc ctatgtcagt agaagtaata atgccaaaag caggagtagc catggaagaa
    28621 ggtactatag tatcttggct aaagcaagaa ggcgaagaag taaagattgg agaacctata
    28681 ctagaaataa caacagataa agttaatatg gagatagagt cagaaggaga aggtacactt
    28741 gctgtaatca tacataaaga ggaaggtgaa gtattaccag tatttacagt aataggtgta
    28801 atagcagaaa aaggggaaaa tcaagaggaa gtaaaagcta aatatttatc tggaaatgtt
    28861 tcaaaagaag atatagttga agagaatcaa aatatagaag ttaaagaaga aaaaataaac
    28921 aaaaaagagt gtaatcatga ttatgatgta gtggtaatag gaggaggacc tgggggatat
    28981 ttatcagcat taaaagcagc tcttttaggt ggaagagttg ctttagttga agaaaatata
    29041 ctaggaggta cttgcttaaa tagaggttgt atccctacaa agacttatat aaaaacagca
    29101 gaaatattag aagaaattga ccaattatca aagagaggtg taaaagtaac tgtagataaa
    29161 gaacaggaca ttaagaaggc cataaaatat aaaaatagag tagttaaaaa acttacagct
    29221 ggtgttggcg gattattaaa aagtagagat gtagatgtgt ttaatcttaa agctagtgtt
    29281 aaagaagagc ataaagtaat tttatcagat ggaaaagttt tagatacaga aaatataata
    29341 attgctacag gttccaaagt aaggatatta cctataaaag gaatagaatc aaatttaata
    29401 ataactagta cagaagcatt agatttggaa acagtgccag aagaattagt aataattggt
    29461 ggtggagtga ttggttgtga atttgcagag atattcaatt caagaggttc taaagtaact
    29521 atagtagaaa tggaagatag agtaatccca agaatggata aagagttaag cgaatcttta
    29581 aaatattctc ttagtaaaaa aggtataaat gtattaacta aaaagaaagt ttctgaattt
    29641 aaagaagaag gaaataacat attagtgtgc attgaaggtg aagtgccaat aaaagcagat
    29701 ttatgtctat atgctatagg aagagaagct aatctttctg gcatagagga cttagatatt
    29761 aaaatagata aaggttcaat agttgtaaat agtaaaatgg aaacaagtat accaagcata
    29821 tatgcagtag gtgatgttac tggaggtgta atgttagctc atgctgcttt taaaatggga
    29881 gaggtagcag cttcaaatgc tctaggtgtg aataaagaag ttgacttagg tgcattacct
    29941 agttgtgttt atacaattcc agaagtagca tcagttggta ttacagaaga ggatgctaga
    30001 aaaaaatata atgtaaaagt tggtaagttt aattttgctg gaaatggaag agctttagct
    30061 tctggtcaag aacaaggata tgtgaaagta gtagcagatg ctaaatatgg agaaatttta
    30121 ggtatacata tgtttggatg tggtgtagca gaacttataa atcatgcagc atcatttaaa
    30181 gcattagaga tacctactga tgaagcaagt gagttgatat ttggtcatcc atgtacttca
    30241 gaagctttaa tggaagcttt ggcagatgta aatggagaat gtttacactt acctaaaaaa
    30301 taaatctaaa cataaattat taatttaagt ctattattaa tattaattta tatcataaaa
    30361 ttttaaaaca cttacttagc catacccctt attatgcaaa taatttattt gtataataag
    30421 gggtattttt tatggaaaaa tatagaatat agaatccttg tagtttttgt tgtacaaaaa
    30481 agatagtaat ataaaataac attatcattt tagaatgatt tcaattgtaa ttaggaaaag
    30541 tgattttttt gtttttaaaa aaagtattat ttgtaaaata t
//
LOCUS       DAGQWR010000036        30516 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.036, whole genome shotgun sequence.
ACCESSION   DAGQWR010000036 DAGQWR010000000
VERSION     DAGQWR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30516
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.036"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            275..727
                     /locus_tag="MIW95_001396"
     CDS             275..727
                     /locus_tag="MIW95_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBY3150727.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(884..1501)
                     /locus_tag="MIW95_001397"
     CDS             complement(884..1501)
                     /locus_tag="MIW95_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBY3150728.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFIYIYMGKEFGAKRNNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDREQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            2050..4710
                     /locus_tag="MIW95_001398"
     CDS             2050..4710
                     /locus_tag="MIW95_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBY3150729.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVSI
                     VEQVMNDGSISSTFNEGHLTVEGEDAYCIDINTAFKNGYKTKADASTRMSADQIADVA
                     LSIEYVKQYTDIHSGISSKHAYLLRQLVVWQRLSVQPDWQWDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNTKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLTTLTTDTSGNTEAVEVRATIVYIKELSAPAGFKIDKT
                     VYSLSVEVGKTATLKVSDTPKVTDTLIELFKIDMETQKSNPQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAIHYITRLADKYKVSWDSFYTQDGKNVLPL
                     GTLTVEETKSPSGYLLEGTYMQADGSEEQIKGMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETKDTKAQGGATLKDTAFEIISLNENAVLVEGKLYKKNEVLKTIH
                     TDIEGIASTSADLLPYGKYRLSEQKPPEGYLTDGAKPIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALLYDTYIIEELRCESNKGFKLIPPFEIVISRN
                     KVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGKDVTITDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIINDKRIENDYIFTADSETMEVKIEFTFDASNLGGKQLVTFEELYD
                     LSNPDEPIKVAEHKDIEDDGQTVTIKEVPETPTPEEPEKPTTPDTPTKTDSPKTGDNT
                     NILAFAIMMFVSAGGLAGTYFFKHRKMKKS"
     gene            4935..5258
                     /locus_tag="MIW95_001399"
     CDS             4935..5258
                     /locus_tag="MIW95_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBY3150730.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQRINGRYTLVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            5273..5656
                     /locus_tag="MIW95_001400"
     CDS             5273..5656
                     /locus_tag="MIW95_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBY3150731.1"
                     /translation="MRLTNGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGTTVDWYIKAD
                     DIVLKNKGGQAGNPQNNAPQGQQKK"
     gene            5752..6165
                     /locus_tag="MIW95_001401"
     CDS             5752..6165
                     /locus_tag="MIW95_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBY3150732.1"
                     /translation="MLDKIPNAEEMTALVGQSLYSVWNELCALIDEKYEMECLWNKGG
                     KAWNYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTYRDNYSKEVQRIYDET
                     KTYHDGKWLMFEPTDTTLFDDFVKLLRIKRKPNRK"
     gene            6272..6730
                     /locus_tag="MIW95_001402"
     CDS             6272..6730
                     /locus_tag="MIW95_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY3150733.1"
                     /translation="MKLKNPMLVVTDIDKSVEFYKKVFGLYVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGNNFEVYFEEEDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            6755..7297
                     /locus_tag="MIW95_001403"
     CDS             6755..7297
                     /locus_tag="MIW95_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBY3150734.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKRRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            7333..7860
                     /locus_tag="MIW95_001404"
     CDS             7333..7860
                     /locus_tag="MIW95_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HBY3150735.1"
                     /translation="MTNGGIPLETVELRFQYTQSEYIKAERQYLISSKTIHKYDIALV
                     AVFLLLSVVYMLFSSFSVFSILIFGLIIVVTALGSYLYILMPILKFKQTAKYHEEYTL
                     VFSKENIKFKTQSIDSEVKWDIYSALWESHDFYYLIQAPRLYTLIPKRIFKDLNEKQL
                     FEEIAQSRVKTTKHI"
     gene            7970..9364
                     /locus_tag="MIW95_001405"
     CDS             7970..9364
                     /locus_tag="MIW95_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY3150736.1"
                     /translation="MRMILNKGHRIRARDKNLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLDNGLTLSPYNFITILIATGICALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMENGLLHIHCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRISIDEVKAENGCLRLMKNLV
                     WEYDVLPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEDMIDCVNAFYEGMVQRSEEMKQHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAFVRQ
                     DGTATCEAKGDGTD"
     gene            9644..10765
                     /locus_tag="MIW95_001406"
     CDS             9644..10765
                     /locus_tag="MIW95_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBY3150737.1"
                     /translation="MAGISREHLSRIEAGKVALTEDMKHKLLEAVEKFNPDAPMFLLF
                     DYVRIRFPTLDIQHVIKDILKLNIDYMLHEDFGHYKYTEHYYIGDVFVYTSQDKEKGV
                     LLELKGKGCRQFESYLLAQERSWYDFLMDALVDGGVMKRIDLAINDRTGLLDIPELIE
                     KCKNEECISKFRSFKNYGSGELVKHNEANKDGMGHTLYIGSFTSEVYFCVYEKNYEQS
                     SKLGIPIEEVPIKNRFEIRLKNEHAYYAIRELLTHYDAELTAFSIINQYIRFADKEAN
                     KRKSDWKTNARWSWFIGEGRPPVKLTTKPEPYTLQRTLNWLQRQVAPTLKMLKQIDAG
                     NQMDYLKTIEQQAKLTDKHKQIIRQQTASVDELIEKEKE"
     gene            10772..10906
                     /locus_tag="MIW95_001407"
     CDS             10772..10906
                     /locus_tag="MIW95_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBY3150738.1"
                     /translation="MWELIKDLLLVSLGMGIGVVLMCIMSVGKEADRHMIELKESEEK
                     "
     gene            10907..11128
                     /locus_tag="MIW95_001408"
     CDS             10907..11128
                     /locus_tag="MIW95_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150739.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALVAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            11448..12086
                     /locus_tag="MIW95_001409"
     CDS             11448..12086
                     /locus_tag="MIW95_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HBY3150740.1"
                     /translation="MEYKYTKIDLNKWNRGKLFQSYIDNMRIVMSLTVDIDVTKFIEF
                     SKKHDLKFYPAMIWAVSKVVNEHDEFKYSWDNNGNLIKWDYISPSYTEFHKEDENFTK
                     IVTDFSDSIFEFHKRFILDREKYKTERAFVENQSLNFFDVSCLPWVKYNHFDVHVFDE
                     GKFLAPVITWGKYELEQERYIMPLTMNIHHAVADGFHLSRFFNEVQELINSI"
     gene            12163..12351
                     /locus_tag="MIW95_001410"
     CDS             12163..12351
                     /locus_tag="MIW95_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150741.1"
                     /translation="MKDIFSDMQASIGCTYISDLPHHKRMVWQEMKRLNLADYPQKQL
                     EDFAGYVFGVPYTSIAEA"
     gene            12372..12869
                     /locus_tag="MIW95_001411"
     CDS             12372..12869
                     /locus_tag="MIW95_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBY3150742.1"
                     /translation="MNEMRVCIESRLHTSEEIRSAWFTLPIDEEELEEQIGVPIDSED
                     YHIVEKELPFADEVGEDATIEKLNDLYHTFESLPADLQEDYGELMCYFTSLDELHQHR
                     HDVIHYSWCKNMADVARHLLDNDPAFSAIEERLTRYFDYEAYGQYLDDNGEFIETDHG
                     IYELP"
     gene            12885..13388
                     /locus_tag="MIW95_001412"
     CDS             12885..13388
                     /locus_tag="MIW95_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBY3150743.1"
                     /translation="MVEDMRVYIANLGKYNEGYLVGDWFSFPIDEEDVAERIGLNERY
                     EEYAVHDTENFPIEIGEYISIEELNEMYEMICELPDYITDSLDEFVSNYGSLEELYDH
                     RDDMILYAGCEDMTDVAYYFIDELQALGDIPPSLQNYIDYEAYGRDLSFEGTFIETRY
                     GMCEIPW"
     gene            13501..13890
                     /locus_tag="MIW95_001413"
     CDS             13501..13890
                     /locus_tag="MIW95_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBY3150744.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDLPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITFALRPKITY
                     AGKAVKLDKQVLHEAITAVRSVSYVPD"
     gene            13877..16309
                     /locus_tag="MIW95_001414"
     CDS             13877..16309
                     /locus_tag="MIW95_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY3150745.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSSRSIQEQSKKLVTGRLKDVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTDQSMNLKSVKQSAFLTFKEFIHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLDKNDFGYLIEHIYGRDGVAYEDYEYSLPKKKLKKE
                     TLITHYDLIRPTRCLVEESQRHLRLEHEDSESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALSTVRNKKKELKDLDNHAYQSGSETSSNVVDALDSVD
                     ELETDLDQSRESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYIGYSVDTGRNVYLQPS
                     LASQGVKDTVTNALASAFVESLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDRENAGLLDPFVIMQNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRAVTQSDQRGLLHVIEELRKEDTPISCNIADHIDSFTDYDFAHLLFSDGTV
                     QNAISLDNQLNIIQVADLVLPDKDTTFELLSVSMLIVISTFALDFIHSDRSVFKIVDL
                     DEAWAFLNVAQGETLSNKLVRAGRAMNAGVYFVTQSSGDVSKESLKNNIGLKFAFRST
                     DINEIKQTLEFFSIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFEELLHAF
                     DTRPPIQRNEVE"
     gene            16309..18444
                     /locus_tag="MIW95_001415"
     CDS             16309..18444
                     /locus_tag="MIW95_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBY3150746.1"
                     /translation="MKERIKSVFTRKKIFRFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYADNNWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     RLYLSNATGYVVQEAYKLDFINDMADSIGRSIQTLAGVTQNGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKVNDFSSDISTAS
                     LDLGTKIMLPDSDSKGKDSVDLIRDSLFSIQVQQPWLLLQFGNSGIEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTILQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVYFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRLARAVSAGGVAGAVAGGVVASSKSTRQNGTKNNRGNTSSSMGQK
                     AGSAVGAVLDTKNKVKDKATAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGVVQE
                     KENRQSGRLKKQQLHRQNIADKRMELQKAQESRQAGKKTDGSATSGATRPHERPATTS
                     KVNVEKQQEVKRPAIASTVKNDEPIKKNVVKERPLSSGDTSAKTVNQETTRQNYKTDR
                     VTKTTVQKHTQQNTEKNKRTVFKKGQKKK"
     gene            18441..19448
                     /locus_tag="MIW95_001416"
     CDS             18441..19448
                     /locus_tag="MIW95_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBY3150747.1"
                     /translation="MKLRHIAIVGSLFSILFSLVLFFGVLISAEDDDGGNGYSSNYVG
                     MNLSAEVLKHQPMVEKYAKENGISEYVNVLLVIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGISDINVVIQSYNYGGGYVGYVAGNGKKHMFNLA
                     ENFARDKSGGQKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLVVNQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYNKAGVSLPRTAQNQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNTQMYHAGDPIGYADLNNAYWQQYLICAGRIK
                     Q"
     gene            19464..20357
                     /locus_tag="MIW95_001417"
     CDS             19464..20357
                     /locus_tag="MIW95_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBY3150748.1"
                     /translation="MFKKKEKPVKETKVRTMKVGTHKKSVIALWVVLIASVSFGVYKN
                     FTAIDMHTVHETETIQLRLNDTNGVENFVKNFCKSYYTWDNSKEAIEARTQAISNYLT
                     KELQDLNLDTIRTDIPTSSTVTNVLIWDIKQSGTNDFIATYEVDQQIKEGEQTSNVKA
                     TYTVKVHVDKDGDMVIVQNPTIAPAIEKSDYEPKAQEADASVDADTVNDATAFLETFF
                     KLYPTATEKELAYYVAGNVLEPIGRDYLYSELVNPMFTDDRDNVKVRVAVKFIDNQTK
                     AMQVSQFELVLHKDSNWKIIM"
     gene            20570..21427
                     /locus_tag="MIW95_001418"
     CDS             20570..21427
                     /locus_tag="MIW95_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY3150749.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERNSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPAWSHRFMGLIQLGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFRFDYTNKVFDLIHVPKKELLTLELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            21505..22086
                     /locus_tag="MIW95_001419"
     CDS             21505..22086
                     /locus_tag="MIW95_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY3150750.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFSLYGLKKVTVDDLAAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTVKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEGLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMVQNLLVNGIIQQVVAE"
     gene            22190..23035
                     /locus_tag="MIW95_001420"
     CDS             22190..23035
                     /locus_tag="MIW95_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3150751.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KILTGLNTSYKGSVKVAGTEIREHTNRFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     DKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNNRLMKDIILAEKKRGKTIIITTHNMYDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIHYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            23032..23715
                     /locus_tag="MIW95_001421"
     CDS             23032..23715
                     /locus_tag="MIW95_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3150752.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALTGHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTIALIAISTI
                     IAVILGFVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPDIFRFFPFTACMNLITGKSILLSFDMVLVIATLIILYIVARHTVKHM
                     WRSLGGVKL"
     gene            23712..24410
                     /locus_tag="MIW95_001422"
     CDS             23712..24410
                     /locus_tag="MIW95_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3150753.1"
                     /translation="MKRILSSTIQVFKQIKSDPMMFAACFTPFVMGALIKFGIPFLDG
                     ITDFSLQTYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLMVALVW
                     YYFLIKRLFRKLAG"
     gene            24422..25333
                     /locus_tag="MIW95_001423"
     CDS             24422..25333
                     /locus_tag="MIW95_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBY3150754.1"
                     /translation="MEKGRLLQNENINKAIQVSKITLIINLGLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDKEHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVSSGIALTAAVLSIVIKEWMYWYTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASIVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELPYSP"
     gene            25386..25526
                     /locus_tag="MIW95_001424"
     CDS             25386..25526
                     /locus_tag="MIW95_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150755.1"
                     /translation="MEQYWWIIIVMVLIVVGLLKIVIMKKYNQRRNSNNRSKNSEDDK
                     MY"
     gene            25641..25832
                     /locus_tag="MIW95_001425"
     CDS             25641..25832
                     /locus_tag="MIW95_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBY3150756.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            25856..26086
                     /locus_tag="MIW95_001426"
     CDS             25856..26086
                     /locus_tag="MIW95_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150757.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            26352..26642
                     /locus_tag="MIW95_001427"
     CDS             26352..26642
                     /locus_tag="MIW95_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150758.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELRRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKNVTMY"
     gene            26642..26776
                     /locus_tag="MIW95_001428"
     CDS             26642..26776
                     /locus_tag="MIW95_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY3150759.1"
                     /translation="MYYFLEMSDTEIADIMNYTREAVFKHRHRALETMKEILNEKTRQ
                     "
     gene            26757..27938
                     /locus_tag="MIW95_001429"
     CDS             26757..27938
                     /locus_tag="MIW95_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBY3150760.1"
                     /translation="MKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYSWKLVP
                     TDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRNTEL
                     GRQYLVNALENDPLGMRAIDTVKQSDAKEWAIRMNGKGYSYKTIDNYKRSLKASFYMA
                     IQDDCIRKNPFNSKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVILLL
                     ETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISKAKTKNGKRELPLTERAY
                     QALKRILKNRGKAQPLVVGGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHKDKL
                     PNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELERLA
                     S"
     gene            complement(28278..29090)
                     /locus_tag="MIW95_001430"
     CDS             complement(28278..29090)
                     /locus_tag="MIW95_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY3150761.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRIL"
     gene            complement(29166..30401)
                     /locus_tag="MIW95_001431"
     CDS             complement(29166..30401)
                     /locus_tag="MIW95_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBY3150762.1"
                     /translation="MGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITSGVTGMF
                     CHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLRGSLASLLEVESGSNDPIAY
                     MLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFYTIF
                     ITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIMLFF
                     MLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVGLRG
                     AASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTPVLK
                     TFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGSTII
                     KTGDILVLSGDRIEELIQL"
BASE COUNT         9956 a         5415 c         6205 g         8940 t
ORIGIN      
        1 atgtttatta ttttttatgg ataccacatt taaaatattt attaatttaa tataaatatt
       61 ttccgttatt attaaaagta gctatatttc gttaatattg tatcactatc gtgtagcaat
      121 aacaataagt acattttata tattttttat aatattattt aatcatccaa acttttacca
      181 ctttttcaat atataaaata ttacacttta gatgtaacaa aatcgcatcc atttaaatat
      241 tgtattttat agactaattt taggatgtga tactttggtt aatttaaatg caaataatct
      301 aaatgtaaat atggtttatc ctatttcttc cacagcaccc atacgtttca gaagatatac
      361 tgtttcatat ttagaaaaca tggaaaatat ttatttaact atagcaacat actttgatac
      421 acttggttta ggcacaatga atatcgacaa agtttatggt caatgggctt ggtttactga
      481 taatatatat gatttaaatc tatttgtttt tgtaggaaat tatccttatc aaattgctaa
      541 aaacagatat gatacatttg tagatatttt gccagtagct attacttcta ttgtcaatgg
      601 agatagagta tttttaattt caaatccttg tttgttaaat tcaaaaatta cagttagatt
      661 tatatctagt catcctactc ttaatagaac agttgattat ggcaatatta gagattttat
      721 tttttaaaac tgcaacatga tgtgctatta aaaaataccc taaatttaat gatattatta
      781 aataactaga atatttttaa ttggcacatc atgcctagct attataaatt cataatatta
      841 ttttacttaa gataagtact gtattttatc tacttgtata tttttattta cctagtttct
      901 cagttctata tcctatttct ttattagcta gcttataacc ttcttgtatc atttcttctc
      961 tcgttaatgc actccctaat aaattgtcaa tactcagttg ctctctatca ctaaaatact
     1021 tatccaataa tgattcctct atatcttcct gatatgccaa atttccttca tgcattatat
     1081 attttacatc gaaaccttct ttatttaata cttttcccac caaaatactt ctatgacatc
     1141 ttataggttc ttgcatagct cctaataaaa ctatcctata acccatttga caaccctttt
     1201 ttaatctttc tattccgttt aaaaaaatat cttcttttgc tactttctca aaatctgcat
     1261 aaccttcttg cgtatataca tctttattat ttcttttagc tccaaattct tttcccatgt
     1321 atatatatat aaatccggaa tttctaagag attcgttaaa tgcctcttta ttgtattgaa
     1381 tattatattt agaatatgga gtttctctaa tgtcaactac acaatttatg tcatattttt
     1441 ttatcatatt aataagctta tcatatggat aatttgaatg tcctattgcg aatatttcca
     1501 tataaatacc ctcctattct ttgaaattag tatctatata tgtagatacc cgatttactt
     1561 aaaattaaat aaagtattac tttaaatacc caatatcttt ttttcttgat actgtatatt
     1621 tttttacttt atatccttaa aaaaatatac aactttatat ataaaacatc aatatttata
     1681 tatattttgt atattaaata caattattta taaaatcttt gataaaaatt tataaataga
     1741 gcatctttag aaaactatta acagaaaaac acactaaaaa tttgattatc cgctaaaaca
     1801 ctgtatctat cggcattatc aaggagctaa acacatcaaa atactactaa tttactacga
     1861 ttgaatgagc cttgatacgt cctatttccc agatatgcaa agatacggta cagcacatca
     1921 gtattgaaac aagaaaaatt gaatacttca tagactacgg aacgcacaat atcagcgttc
     1981 cttttctatt tccagccgtt cctagtggac ggctatttta ctaccaaaat gaaaggagca
     2041 tttagaatta tgaaaaagat attaaaacga ttgtgtacag gctttcttgc ctttgcaact
     2101 gtcgttaccg ctttaccgag ttcaacggtt catgcgtcta atacacagta ttggacggaa
     2161 agtaaagagc gtgtcagtat cgtagaacaa gtaatgaatg acggttctat cagttctacc
     2221 tttaatgagg gacatttaac cgttgagggt gaggacgctt attgtatcga catcaacaca
     2281 gcctttaaaa acggctataa gaccaaagct gacgcaagta cccgtatgag tgccgaccag
     2341 atagctgacg tcgccttatc tattgagtat gtgaaacagt acacagacat ccacagcgga
     2401 ataagcagta aacacgccta cctattaaga cagcttgttg tctggcagag attaagtgtt
     2461 cagcctgact ggcaatggga taatgtgcga gcttcctatg atgaaatccc aaaagccgta
     2521 caagatgaag tattcgcagg agcaaaagcc tttgtgaaag aaaacaaagg acgttatgac
     2581 tgtggcggat atatctactc tggcgagggt caagagttag ggcaattctg ggcgaagtta
     2641 gctgtcggaa ataccaaact tcaaaagact tccaccaatg ccaatattac agacggcaac
     2701 gggatttatt caatcgctgg tgcaacatac ggtgtgtatt ctgataaaga ctgcacgaaa
     2761 cagcttacca cccttacgac tgatactagc ggaaatacag aagccgttga agtaagagcc
     2821 actatagtat atatcaagga actttctgca ccagcaggat ttaaaattga taaaacagtt
     2881 tactctttat ctgtggaagt tggaaaaaca gcgactttaa aagtatctga tacaccaaaa
     2941 gtaacagata ccttgattga gctattcaag attgacatgg aaacacagaa atccaatccg
     3001 cagggaaacg cttctttaga gggagcagag tttacatgga acttctacgc tggatattac
     3061 aacaaaaaca atcttccagc acagcctaca agaacatggg taacaaagac tatcgctgaa
     3121 aaagacagcg acggtgctat ccattacatt actcgtttag ctgacaaata taaagtatct
     3181 tgggacagct tctatacgca ggacggtaaa aacgtacttc cattaggaac actaactgtt
     3241 gaggaaacaa aaagtccgtc gggttacttg ttagagggta catatatgca ggctgacggt
     3301 agcgaagaac agataaaagg aatgtatctg acacagatta cagaggacgg cgaccttgcc
     3361 gtattatctg gaagcaatca ataccatgtt tctgataaag tcattcgtgg cggtgtgaaa
     3421 atccagaaac gagatttaga aaccaaagac acgaaagctc aaggcggtgc gactttgaaa
     3481 gataccgcct ttgaaatcat ttccttaaat gagaatgccg tcttagttga gggcaagtta
     3541 tacaagaaaa atgaagtgtt aaagacaatc cataccgaca ttgagggtat cgcttctact
     3601 tctgctgacc tattacctta cggaaaatac agattgtctg aacaaaaacc gccagaggga
     3661 tatttgactg acggtgcaaa gccgattgat tttgaaatca cagaaaatgg aaaaatcgtt
     3721 gatttgactg acgaagccca ctctatctat aaccagatta aacgtggcga tattgagggc
     3781 gtaaaaattg gtgcaggttc gcacaagcgt cttgcagatg ttccgtttag gatcacaagc
     3841 aagacgacgg gcgaaagtca tgttgttgta actgatgata acggacaatt ctctactgct
     3901 tctgattggg cttcccacaa gcacaatacc aatgcaggaa aaacaagcga ggacggtgtg
     3961 tggtttggta cttctgaacc agacgacagc aaaggtgcgt tgctttatga tacttacatc
     4021 attgaagaat tacgttgtga aagcaacaaa ggtttcaaac ttattccacc gtttgagatt
     4081 gtcatttcca gaaataaggt tgtggttgat ttaggtacgc tgactgatga atacgaaaaa
     4141 gaaatcacta tccatactac tgctaccagc aaggacggcg aaaagactat tcttgctggg
     4201 aaagacgtaa ctatcactga tacagttaaa ttagacggtc ttaccaaagg gacaaaatat
     4261 cagttgaaag gctggcagat gttaaaagaa gaaaatgcag agcttatcat caatgacaag
     4321 cgtattgaga atgactacat ctttacggct gacagcgaaa ctatggaagt taaaatcgag
     4381 tttacctttg acgcttccaa tcttggcggt aaacagcttg taacttttga ggaattatac
     4441 gatttaagca atccagacga acctatcaaa gttgcggaac acaaggacat tgaggacgac
     4501 ggacagacag ttactatcaa ggaagtacca gaaacaccta ccccagaaga accagagaag
     4561 ccaaccacac cagatacacc tacgaaaaca gacagtccca aaacgggcga caatacaaat
     4621 atccttgcct ttgcaattat gatgtttgtt tctgctggtg ggctggctgg tacatacttt
     4681 ttcaaacacc gtaagatgaa gaaatcataa ggcggtagct gtatgagaaa agaaaaatca
     4741 cgctctccgc catagcttag ataggcagac tatacctatt ctggcttgtc tgccgattac
     4801 ctgcaagaac acggatagtc aagggtgcgt tagcatttat ttacccttta ctatctgggg
     4861 gcttgctggc actttcctaa atgccagaat acacacatca atcaaactta aagtaagaaa
     4921 cgaggtaaaa taatatggaa ttaaaatata tcgtgccgaa tgtggcacag acttttggaa
     4981 acttggaatt tgcaggggaa aacaaagtag accagcaacg tatcaatggg cgttacactc
     5041 tcgtatcaag aagttacaac ttgtattcgg atattcagag agccgacgac attatcgttg
     5101 tattgcctgc aaaagctggc gagaagcatt ttaagccaga gcagaaagta aaactgatta
     5161 acccacgcat taccgcagag ggttacaacg tcaaaggacg tggatttaca aactacattt
     5221 taatggctga tgatatgtta ccagcagaaa gcaaataagg gggtatttga ttatgagatt
     5281 aacaaatgga attgtattag ataacgaaaa aacttttgga gtattgaaat tctctgcact
     5341 tcgacatgag gtgcgtgtag aaaatgagga cggaacgaca agtgatgaaa ttaagcgtcg
     5401 tacttatgat ttgaaatgta gtgtgcagga acgcatgata caagtgtcaa tccccgctga
     5461 tgttcccgta aaggactttc cttataatgc ggaagtggag ttaatcaatc ccgtagctga
     5521 tacggtggca aatgccaact ttagagggac tacggttgat tggtacatca aagctgatga
     5581 tattgtattg aagaacaaag gcggtcaagc tggaaatccg cagaacaacg ctccgcaggg
     5641 acagcagaaa aagtaagctg acaaactagt ttttcattgt aggagatata aacaactgtt
     5701 ataatatgga tgtggaaaca gttaatttag acttataaag gagaaatgtg tatgctagat
     5761 aaaataccta atgcagagga aatgactgct ttggtcggac aatcattata tagtgtatgg
     5821 aatgaattgt gtgccttgat tgatgaaaag tatgaaatgg aatgtctatg gaataaaggt
     5881 ggcaaagcat ggaattatga atataagtat cgtcgaggtg gcaaaacatt atgtgcctta
     5941 tatgctagag aaaattgtgt gggatttatg attatcttag gtaaagatga acggttaaaa
     6001 ttcgatacat atagagataa ttattcaaaa gaagttcaga gaatttatga tgaaacaaaa
     6061 acttaccatg acggaaaatg gctgatgttt gagccaacag atacaacttt gtttgacgat
     6121 tttgttaaac ttttgaggat aaaaagaaaa ccaaatagaa agtagtttat taccacacca
     6181 ttagcaaatg gatagtttct tataatagcg gatatatgaa aatgctgtca tgtggacata
     6241 aagaaaattg gaagttgtgg aggtagcaaa aatgaaatta aaaaacccta tgttggtagt
     6301 aactgatata gataaatcag ttgagtttta taaaaaggtt tttggacttt atgtcattat
     6361 ggattttggg gcaaataaaa ctttaacagg tggattagcc ttacagacaa gtgaaacata
     6421 caaagagttt atcggtacaa gtgatatttc ttttggtggt aacaattttg aagtctattt
     6481 tgaagaagaa gattttgata aatttgctga taggctgaaa gaatatgata ttgaatatgt
     6541 ccaccctatt atagaacatt catggggaca gcgtgtagtc cgcttttatg acccagataa
     6601 acatattatc gaagttggag aaaacatgaa gatagtgtgc aagcgtttct taaatagtgg
     6661 aatgacacca gaacaagttg cagaacgtat ggatgtacct atgaaattta taaatgcttg
     6721 tatgcgataa attgaaagtt aggagactaa tactatgaaa ggatttgaac gcaaaaatca
     6781 attattttct ctttgcggat tgaattgcgg actatgccct atgcttttgg gaaattactg
     6841 tggcggttgt ggcaatggta atcaatcatg cagaattgca aaatgtagtc ttgaacatgg
     6901 acagattgaa tattgctatg aatgcaaaca atatccatgc gaaaaatatc aacacattga
     6961 tgattatgat tctttcatca cgcataaacg gcgaaaagca gatttagaaa gagcaaaaaa
     7021 cataggcatt gagcaatata atcttgaaca gcaggaaaaa acacaaattc tttcttactt
     7081 actttctaac tataatgacg ggcgtagaaa gactttcttt tgtgtggcag tcaatttatt
     7141 agaactttca gaattacaag aagcaatgaa acaaatacaa tccaatgacg aattgacctt
     7201 actaccattt aaggcacaat gcttgtatgt ggtggaggtg tttcagaaga ttgctgaaag
     7261 gagaaatata aaactgaaac ttttcaagaa aaagtgattt tcaaatttga aattgtaaca
     7321 aagattattt atatgacgaa tgggggaata cctttggaaa cagtagagtt aagatttcaa
     7381 tatactcaaa gtgaatatat aaaagcagaa aggcaatatt taatttctag taaaactata
     7441 cataaatatg atattgcttt agtcgctgta tttttgttgc tgtcagtcgt ttatatgctg
     7501 ttctcctctt ttagcgtatt tagtatttta atatttggat tgataattgt tgtaactgca
     7561 ttgggaagct atttatatat cctaatgcct atactgaaat tcaaacaaac tgcaaaatat
     7621 catgaggaat atacgctggt attttctaag gaaaacatta aatttaaaac acaaagcatt
     7681 gactcagaag tgaagtggga catttactct gcactatggg aaagccatga tttttattac
     7741 ttaattcaag caccacgatt atatacgtta attcccaaaa gaatttttaa agacctaaac
     7801 gaaaaacagc tatttgaaga aattgcacaa tcaagagtta aaacaacaaa gcatatataa
     7861 tgaacatacc ctacaatgta acaacttcat attcatacac acaaagcggt tagtctttta
     7921 agattgactg cttttttcat acttaaatta agattggagt gattgattta tgcgaatgat
     7981 tttgaataaa ggacaccgta tcagagccag agacaagaac cttgtctacc acttttccat
     8041 agggacgctt ctgtttgtat ttgtggcggt tctcctgcta ctgaatatca aacagctcat
     8101 gcgtaccgac tgggaacatt tcagcttatt ggacaatggc ttgacgcttt ccccctacaa
     8161 cttcataacc atattgatag cgactggtat ttgtgcattg gtcgcttttc tgtattaccg
     8221 cttctgttat gacagtttca aaaagctcct gcaccgtcaa aagctggcac gaatgatact
     8281 ggaaaacaag tggtatgaag cagataccgt acaagacagc ggttttttta ctgacctgca
     8341 aagcagatca agggaaaaaa tcgtctggtt tccaaagatt tattatcaaa tggaaaacgg
     8401 attgcttcat attcactgtg aaatcacatt aggaaaatat caagaccagc ttttgcgatt
     8461 agaggataag ctggaaagcg gtttgtactg tgagctgacc gacaagacct tgcatgacgg
     8521 ctatattgag tacacgcttt tatatgacat gatagcgaac cgtatttcca ttgacgaagt
     8581 gaaagcagaa aacggctgtc ttaggctgat gaaaaatctt gtctgggaat atgacgtact
     8641 ccctcacgct ctgatagctg gtgggactgg tggcggtaag acctactttc tgctgacgct
     8701 tattgaagct ctgctacata cgaatgctgt cctttacatc ttagacccaa agaacgctga
     8761 ccttgcagac ttaggtacag ttatgggaaa tgtgtatcat accaaagaag atatgattga
     8821 ttgtgtcaat gccttttatg agggcatggt acaaagaagc gaagaaatga agcaacaccc
     8881 agactataaa acgggcgaaa actacgccta tcttggttta ccgccctgct ttcttatctt
     8941 tgatgaatat gtggcgtttt ttgaaatgct ggggacaaag gaaagcgtaa gtttacttag
     9001 ccagttaaag aaaatcgtta tgttgggacg acaagctgga tatttcctta ttgttgcctg
     9061 ccagcgtcca gacgcaaaat atttctcgga cggtatcaga gataatttca atttccgtgt
     9121 tggattgggg cgtatcagcg aattgggtta cggtatgctc tttggttctg atgtgaaaaa
     9181 acagtttttc cagaagcgta tcaaggggcg tggctattgt gatgtgggaa caagtgtcat
     9241 aagtgagttt tacacgcctt tagtcccgaa aggacatgat tttttgcaga ccataggctc
     9301 tcttgcattc gtaaggcagg acgggacggc gacgtgcgaa gcgaaaggcg acggcacgga
     9361 ctagccgttg ctggtgtggc gttagccacg ccagcagata taagcccctc attatctaac
     9421 agaggggcac aattacgctg gaaactggtt caaaaacctt gaaaagtccg ctaggaataa
     9481 ggattttctt agtttttagc tagttgtgat aatcacttga aaatatcaca aaatcacaac
     9541 aatatcacga ttgggggtat gacaactgaa tgatactgat tttctgaaca ccttgaaagc
     9601 aaaacgcagt gattatggcg tatcgcagac aaggcttgca atcatggctg gtatcagccg
     9661 tgaacatcta agccgtatcg aagctgggaa ggtagcactg accgaggata tgaagcacaa
     9721 actactggaa gcggtggaaa aattcaatcc cgacgctccc atgttcctgc tctttgatta
     9781 tgtgcgtatc cgatttccca cgctggatat acagcacgtt atcaaggaca ttctaaagct
     9841 gaatatagat tatatgcttc acgaagattt cgggcattac aagtacaccg agcattacta
     9901 tatcggggac gtgttcgttt acacttcaca agacaaggaa aaaggtgtcc tgctggaact
     9961 gaaaggaaaa ggctgtcgtc agtttgaaag ctatctgctg gcacaggaaa gaagctggta
    10021 tgacttcctc atggacgcat tagttgatgg cggtgtgatg aagcgtatcg acctagctat
    10081 caacgacaga acgggcttac tggatattcc agaactgata gagaaatgca agaacgagga
    10141 atgtatttct aaattccgta gcttcaagaa ctatggttct ggggagctgg taaagcacaa
    10201 cgaagccaac aaggacggca tgggacatac gctttacatt ggttcattta ccagtgaagt
    10261 gtacttctgt gtctatgaaa agaattatga acaatcctca aaattaggga taccgattga
    10321 ggaagtcccc attaaaaacc gatttgagat acgtttgaaa aatgagcacg cctactatgc
    10381 cattagagag ctgctgaccc actacgacgc agagctgacg gcattttcta ttatcaacca
    10441 gtatatccgc tttgccgata aggaagccaa caagcgaaaa agcgactgga aaaccaacgc
    10501 ccgctggtct tggttcattg gcgagggacg accgcccgtg aagctgacga caaagccaga
    10561 gccttacact ctgcaaagga cgctgaactg gttacaaaga caggttgcac cgaccttgaa
    10621 aatgctgaaa cagatagacg ctggcaatca gatggactat ctcaagacca ttgaacagca
    10681 ggcaaagctg acagataagc acaagcagat aatcagacag cagacggctt cggtggacga
    10741 actgatagaa aaagaaaagg agtgattgat gatgtgggaa ttgattaagg acttactgct
    10801 ggtatcactt ggcatgggta tcggcgtggt tctgatgtgt attatgagtg tcggaaagga
    10861 agctgaccga cacatgattg aattaaaaga aagcgaggaa aaataaatga atttcgggca
    10921 aaatttatat acatggtttt taagcaacgc ccagagctta gtgcttatgg cgattgtggt
    10981 tattggtatc tacttagggt ttaaacgtga gttttcaaaa cttatcggct tcttggtggt
    11041 tgccttagtt gctgttggat tggtattcaa tgcaggcggt gtcaaagacg tactcttaga
    11101 actattcaat aagattatcg gcgcatagcc gtactttttc attatggttt gcggatacgc
    11161 aaacatacct ttcccacttt gggagtgggc taaggggaaa gggggtaacg gaatggtaca
    11221 cagataaaaa tacagcataa tgaatttcat tgcttggctc aaacatagtt gatataataa
    11281 agatatgaaa ataataaacg aaagtttttt tgataaggag tgatattgtg aaattgtgta
    11341 ttgttttatc tccatataag gcttaatgga atagacttgt atggagtgaa atgttatcca
    11401 gagagcctta cattttatca aatttcaaaa tgtaaaggag aatgggcatg gaatataaat
    11461 atacaaaaat tgatttgaat aaatggaata gaggtaaatt gtttcaaagc tacattgata
    11521 atatgcgtat tgttatgagc ttaaccgtag atattgatgt aacaaagttt attgaatttt
    11581 caaagaagca tgatttaaaa ttttatccag ctatgatatg ggctgtttca aaggttgtta
    11641 atgaacatga tgagttcaaa tatagttggg ataataatgg gaatttaatt aagtgggatt
    11701 atatttctcc gtcctatact gaatttcata aagaagatga aaatttcaca aagatagtta
    11761 cagatttttc tgatagcata tttgaatttc ataaacgttt tatattagat agggagaagt
    11821 ataaaacaga acgtgcattt gtggaaaatc aatctttgaa cttttttgat gtatcttgtt
    11881 taccgtgggt aaaatataat cattttgatg ttcatgtttt tgacgaggga aaatttcttg
    11941 caccagttat tacttggggc aaatatgaat tagagcaaga aagatatata atgcctctta
    12001 caatgaatat acatcatgcc gtagcagatg gattccattt atccagattt tttaatgaag
    12061 tacaagaatt gataaactca atttaacagc tatatacaaa gcagttagtc tatacgattg
    12121 actgcttttt ttcataccta aatttaagat tggagtgatt gcttgaaaga tatattttcg
    12181 gatatgcagg caagtatcgg ctgtacctac atttcagatt taccacatca taaacgtatg
    12241 gtgtggcagg aaatgaagcg actgaacctt gccgattatc cgcagaaaca attagaagat
    12301 tttgcaggct atgtctttgg tgtcccttac actagcatag ctgaagcatg aaagaaagga
    12361 cgtgaaaacc tatgaatgaa atgcgtgtct gtatcgaaag ccgactacac acttctgaag
    12421 aaatccgttc cgcatggttc acacttccca ttgacgagga agaactggaa gaacagatag
    12481 gcgttcctat cgacagtgaa gattaccata tcgttgaaaa ggaactgccc tttgctgatg
    12541 aagtcggcga agatgcgacc attgaaaaac tgaatgacct ttaccatacc tttgaaagtc
    12601 tgcctgccga tttacaagag gactacgggg agctgatgtg ttactttacc agccttgacg
    12661 aactgcacca gcaccgccat gatgtgatac attattcatg gtgtaaaaac atggctgatg
    12721 tggcaaggca tttgttggac aatgaccctg ctttttctgc catcgaggaa cgcctgaccc
    12781 gttactttga ttatgaagcc tacggacagt atcttgatga taacggagaa tttatagaaa
    12841 cagaccacgg tatctatgaa ctgccgtaga agtgaggtgt ttctatggta gaggatatgc
    12901 gtgtatatat cgctaatctt gggaaataca atgaggggta tctggtcggc gactggttct
    12961 cttttccgat tgacgaagaa gatgttgccg agcgtatcgg cttgaatgaa cggtacgaag
    13021 aatacgccgt acatgatacg gaaaactttc ccattgagat tggcgaatat atctcgattg
    13081 aagagctcaa tgaaatgtat gaaatgatat gtgagcttcc cgactacatc acggacagtt
    13141 tggacgaatt tgtatcaaat tatggaagtt tggaggaact gtacgaccat agggacgata
    13201 tgattttata cgctggctgt gaggatatga ccgacgttgc ctattacttc attgatgaat
    13261 tgcaggcact tggcgatatt ccccctagtt tgcagaacta tattgactat gaagcatacg
    13321 gcagggattt atcctttgag ggaactttta ttgaaacaag atacggtatg tgtgagatac
    13381 cgtggtaaga agctggaaga tcagcgacaa gcattgttgc ttctgacagc tttttctatt
    13441 ggggacagtc tgaaaaacgg ctgtcctttt ctattatacg aaaggagttg aaacaaaaac
    13501 gtgaaaaaga ttaaaagcta cacgggtatc tggaacgtgg aaaaagtctt gtatgcaatc
    13561 aatgacttta acctgccctt tcctgttacc tttacacaga ttacatggtt tgtgataacc
    13621 gagtttatca tcattctgtt tggggactta ccgcccttat ccatgattga gggggcattt
    13681 ttaaaatact tcggtattcc cgtagctctt acatggttca tgtcgcaaaa gacctttgac
    13741 ggcaagaagc cgtacagctt tttgaaatca cagataacct ttgccctacg accaaagatt
    13801 acttatgcag gaaaagccgt gaaactggat aagcaggtac tacatgaagc tattacggca
    13861 gtaaggagtg tgagctatgt tcccgattaa atatatcgac aacaaccttg tctggaacaa
    13921 ggacaatgag gtgttcgcct actatgagct gataccttac aattacagtt ttctatccgc
    13981 tgaacagaaa ttcattgtgc atgacagctt ccgacagcta atcgcacaat cccgtgaggg
    14041 caagatacac gctttgcaga ttgccacaga aagctcaagc cgaagcatac aagaacagtc
    14101 aaagaagctg gttacgggac gactaaaaga cgttgcctat cagaagatag acgaacagac
    14161 cgaagcgtta gtatctatga ttggggacaa tcaagtggac taccgctttt ttcttggctt
    14221 caagctcatg gtaacagacc agagcatgaa cctaaagagc gtgaagcaat cggcgtttct
    14281 cacattcaag gagtttatcc atgaagtgaa ccacacgctg atgaatgact ttgtttccat
    14341 gccaaatgat gaaatcaacc gctacatgaa aatggaaaag ctgttggaaa ataaaataag
    14401 ccgacgcttc aaagtaagac gcttagataa aaatgacttc ggctatctga ttgaacacat
    14461 ctacggcagg gacggtgtcg cctatgaaga ttatgagtat agcttaccga agaagaaact
    14521 gaaaaaggaa acgctgataa cgcattatga cttgatacgt ccgacccgtt gccttgtgga
    14581 agaaagccag cgacatttac gcttggaaca tgaggacagc gaaagctacg tttcctactt
    14641 tactgtcaat gcgattgtcg gcgagcttga ttttccgtcc agtgaaatct tctatttcca
    14701 gcaacagcag tttacttttc ccgttgatac cagcatgaat gtagaaatcg tggaaaaccg
    14761 aaaagcgtta tcgacggttc gcaacaagaa aaaagagctg aaagatttag acaatcatgc
    14821 ataccagtct ggcagtgaaa ccagctcaaa tgtggtagac gcattagaca gtgtggacga
    14881 gctggaaacg gatttagacc agtccagaga aagtatgtat aagctctctt atgttatccg
    14941 tgtatctgct ccagaccttg acgagttgaa aagacgctgt gatgaagtca aagacttcta
    15001 cgacgatttg aacgtcaagc tggtacgccc tgctggcgat atgctggggc ttcactctga
    15061 atttcttcct gccagcaagc gatacatcaa cgattatgtg cagtatgtca aaagtgattt
    15121 tctagctggg ctgggctttg gtgcgacaca gcagttaggg gaaaacacgg gtatctatat
    15181 cggctattct gtcgatacgg gacgcaatgt ttatctacaa ccgtcccttg ccagtcaagg
    15241 cgtaaaagat acggttacta atgctcttgc ttctgccttt gtagaaagtc ttggcggtgg
    15301 aaaatcattc tgtaacaacc tgcttgtcta ctactctgtg ctgtttggcg gtcaagcggt
    15361 aatcttagac ccaaaatccg agcgtggcaa ctggaaagaa acgctcccag aaatcgccca
    15421 tgaaatcaat atcgtgaacc ttaccagcga cagagaaaat gcagggcttc ttgacccgtt
    15481 cgtgattatg cagaatgtga aagacgctga aagtctggca atcgacatac tcacattcct
    15541 tacgggcatt tcttcaaggg acggcgagaa gttccccgta cttagaaagg cagtgcgtgc
    15601 cgttacccag agcgaccagc gaggattgct ccatgtgatt gaggaattaa gaaaagaaga
    15661 tacacctatc tcttgcaata ttgccgacca tatcgacagc ttcacggact acgactttgc
    15721 acatctgctg ttctcggacg gtacggtgca aaatgcaatc agcttagaca accagctcaa
    15781 tatcattcaa gtagccgact tagtgctacc cgataaggac acgacctttg aattactgtc
    15841 cgtttccatg ctgattgtga ttagtacctt tgcgttagat tttatccact cggatagaag
    15901 tgttttcaag attgtagacc ttgacgaagc atgggcgttc ttaaatgtgg cacaaggcga
    15961 aacgctctct aacaagctgg ttcgtgctgg acgtgccatg aacgcaggcg tttattttgt
    16021 tacacagtcc tctggcgacg tgtcaaagga aagtctgaaa aacaatatcg gtttgaaatt
    16081 tgcctttaga agcaccgata ttaacgaaat aaaacagacc cttgaatttt tcagcattga
    16141 caaagaggac gagaacaacc agaagcgatt gcgtgatttg gaaaacgggc aatgcttgct
    16201 tcaagattta tacggacgtg tgggcgtggt gcagatacac ccagtctttg aagaattact
    16261 gcacgccttt gataccagac cgccgataca gcgaaatgag gtggagtgat gaaagaaagg
    16321 ataaaaagtg tgttcacaag aaaaaagata ttccgctttc tcaaaatggc tctgtttgtc
    16381 gtggcacttt cccttatcct gctttcactt ttgggaacgg tggcacacgc aacgggactt
    16441 gtggacgaca ctatcaacac cgagaacctt tattccaaat atccgctgtc gaattatcag
    16501 cttgatttct atgcagataa taactgggcg tggttgccgt ggaactggct ggacgggatt
    16561 ggaaaaagtg tgcaatatgg gctttactgt attaccaact ttgtctggac gataagacta
    16621 tatctaagca atgcaacggg ctatgtagtg caggaagcct acaagctgga ctttatcaac
    16681 gatatggcag acagtatcgg aagaagcata cagacccttg caggcgtaac tcaaaatggt
    16741 ttttctactt cgggctttta tgtgggattt cttctgctga ttatcttagt cgtaggtatc
    16801 tatgttgcct atacgggact gataaaacgg gaaaccagta aggcactaca cgccgttatc
    16861 aactttgttg tggtgttcgt attatcggct tcttttatcg cctatgctcc tgactacatc
    16921 aagaaagtga atgacttttc atcagacatc agtaccgctt cactggactt gggaacaaaa
    16981 atcatgcttc ccgacagcga cagcaagggc aaggacagcg tggacttgat aagggatagc
    17041 ctattttcca tacaagtaca acagccgtgg ctactgcttc aatttggtaa ttcgggtata
    17101 gaggaaatcg gggcagaccg tgtcgaagct ctcgtatcgg caagcccaga ggacgaggac
    17161 ggcaagacca gagaggaagt cgtgaaaaca gaaatcgagg acaacgacaa caacaatctg
    17221 acgatactgc aggtggtaaa ccgtttaggt atggtgttct tcctcttgtt cttcaacttg
    17281 ggtatcacga tatttgtatt cttgcttacg ggtatgatgt tgttcagcca gatacttttt
    17341 atcattttcg ctatgtttct gcctatcagc tttctattaa gtatgatacc gagctatgag
    17401 agcatggcaa aacaagccat agtccgtgtg tttaatacga ttatgacaag ggcaggtatt
    17461 acactgattg tcacggtagc ctttagtatt tccagtatgt tctacaacat atcgacggac
    17521 tacccgttct ttatggtggc gttcttgcag atagtgtatt ttgcaggtat ctacatgaag
    17581 ctgggcgatt taatgagcat gttctcttta aacgctggcg acagtcaaag catgggacgg
    17641 cgaatattcc gcagaccgta tctgttcatg cgacataggg ctagacgtat ggaacatagg
    17701 cttgcaagag ctgttagtgc tggtggtgtc gctggtgcgg tggctggtgg tgtcgttgcc
    17761 agttcaaaat ctacacgcca gaatggaaca aagaacaatc gtggaaatac aagctccagc
    17821 atggggcaga aagcaggttc agccgtggga gctgtccttg atacaaagaa taaggtcaag
    17881 gacaaggcaa ctgctgtcaa agaaaatatc aaagatatgc cgacacagac tgcctatgcg
    17941 gttcactctg ctaaggaaaa ggcgaagtcc agtgtgtctg atttcaagcg tggcgtggtg
    18001 caggaaaagg aaaaccgcca gtctggacgc ttaaagaagc aacagctaca tagacagaat
    18061 atcgctgaca agcgtatgga acttcaaaag gcacaagaat caaggcaggc aggtaagaaa
    18121 acggacggat cagcgacaag cggagctacc cgtccccatg aaagaccagc taccacttct
    18181 aaggttaatg tagaaaaaca gcaggaagtc aaacgccctg ccatagcttc aaccgtgaag
    18241 aatgatgaac ctatcaagaa aaatgttgtc aaggaacgtc cactatcttc tggcgataca
    18301 tcagcaaaga ccgtcaatca agaaaccaca agacagaatt acaagactga ccgtgttacg
    18361 aaaacaaccg ttcagaagca tacacagcag aataccgaga agaacaaacg aaccgtattc
    18421 aagaaaggac aaaagaagaa atgaaactaa gacatatcgc tattgttggc agtctgtttt
    18481 ctatcctctt ttctctcgta ttattctttg gggttctgat tagtgccgag gacgacgacg
    18541 ggggcaatgg ttattcctct aattatgtag gtatgaacct atctgccgaa gtcttgaaac
    18601 atcagcctat ggtagaaaag tatgccaaag aaaacggtat ttccgagtat gtcaatgtgt
    18661 tgctggtaat catacaagtg gaaagtggcg gtacagccga ggacgttatg caaagctctg
    18721 aaagtctggg actaccaccc aactcactag atacggaaag ctcaatcaag caggggtgta
    18781 aatattttgc gtccctgctt tcttcctgca aaaatcaagg tatcagtgat ataaatgttg
    18841 tgatacagtc ctataactat ggcggtggct atgttggcta tgtggcagga aacggaaaaa
    18901 agcatatgtt caaccttgcg gaaaacttcg caagggataa gtcgggcggt caaaaagtaa
    18961 cctatacgaa ccctatcgcc gtggcaaaaa atggtggctg gcgttatggc tatggaaata
    19021 tgttctatgt ggagcttgtg aaccagtacc ttgttgtcaa tcaagtatcg ggcgagctgg
    19081 cacaaaaggt tatgaatgaa gccttaaaat atcaaggttg gaagtatgta tatggtggca
    19141 gtaacccgaa cacttctttt gattgttcgg gactggtaca gtggtgctac aacaaagctg
    19201 gcgtatcttt accgagaacc gcacaaaatc agtatgacgc aacccagcat atcccactat
    19261 cgcaggcaaa ggcaggcgat ttggtctttt tccacagtac ctacaatgca ggaagctatg
    19321 taacccacgt cggtatctat gtaggaaaca ctcagatgta tcatgcaggc gaccctatcg
    19381 gatatgccga tttaaacaat gcttattggc aacagtactt gatttgtgct ggaagaataa
    19441 aacagtagaa aggattggaa accatgttta aaaagaaaga aaaaccagta aaagaaacga
    19501 aagtccgcac tatgaaagta ggcacacata agaaatctgt gattgctctg tgggtggtgc
    19561 ttatcgccag cgtatcattt ggcgtgtata agaactttac cgccattgat atgcacacgg
    19621 tacatgaaac ggaaaccatt cagttaagac taaatgacac caacggagtt gaaaattttg
    19681 tgaagaactt ctgtaagtcc tactacacat gggacaacag caaggaagct atcgaagcaa
    19741 ggacgcaggc aatcagcaac tatctgacaa aggagttgca ggacttgaac cttgatacca
    19801 ttaggacgga tataccgaca agctccactg ttacaaatgt actgatttgg gatataaaac
    19861 agtctggaac gaacgatttt atcgctacct acgaagtaga tcagcagata aaagagggag
    19921 aacagacaag caatgtcaaa gcaacctata ccgtcaaagt ccatgtggat aaagacggcg
    19981 atatggttat cgtacagaac ccaaccattg caccagcgat tgaaaaatca gactatgagc
    20041 caaaggcaca agaagccgac gcaagcgtgg acgcagatac cgtcaatgac gctaccgcat
    20101 ttttggaaac tttctttaag ctgtatccga cagcaacaga aaaagaactt gcctactatg
    20161 tggctggcaa tgtgcttgaa cctatcggca gggactacct ctattccgaa ttagtcaatc
    20221 ctatgtttac ggatgacagg gataatgtga aagtcagggt tgcggtgaaa ttcattgata
    20281 atcagacaaa ggcaatgcag gtatcacagt ttgagcttgt gttacataag gatagcaact
    20341 ggaaaattat tatgtaagat attttgttgg caggctctat tttcggatat agtctgcctt
    20401 ttctatagca aatgtgctgc actaacaagc gatttcattg tattttaagc aattaaatct
    20461 ttcttgacaa ttttatctta aatcatatat ttatggatat tgactttctg cttgcaataa
    20521 gttataatat gactataaaa ctaaattagt catataggag gcgtgtggta tgtataaaga
    20581 agttggtatt tcttcaaata atgttccgat aacactttct atttggtata cagaaagaaa
    20641 tagtcctacc attgtttttt tgccgggtac tatgtcacac cctctcatgt atgagaattt
    20701 tctatgtggt ttgagtgaaa gaggatttaa tataataggc attcactatc tttcacacgg
    20761 aaaaagccct aaaatcaaaa aaaattatac aatggaagat ttgctgcata atgtatatga
    20821 cgcaacgact tacggaatag aaaattttgg tgaaaacata gctgtcatgg gttccagtca
    20881 aggtgggatt ttagctgcaa tggcggcggg tagggacaca aggttaaaag ccgtttttcc
    20941 acataatatc atgctgacta cattaaaaga aactatgagc ttaacaaaat accccgcatg
    21001 gtcacaccgt tttatggggc tgatacagtt gggctttcgt gtaggtggaa aattgctgcc
    21061 taactataag gttccaggag acgcttattt agactatgac agagtatttt atagtgaaca
    21121 ggcaaaacaa gattgcttaa atgaccctat gcttttaccc tattatcctt taagatttgt
    21181 cgccagctta tttaatgctg acttatcgtg cttggagaat ggggctatac aatgtcctgt
    21241 tattttaatt actgccaaag gcgatccgct ttttagattt gattatacca acaaagtatt
    21301 tgatttaatt catgtaccga aaaaggaatt acttacttta gagcttaatc gtcacatgat
    21361 ttttaatgag gacacggaca ctacaataaa cgcattggaa gctaccttgc ataaatatct
    21421 tggatagtat attgattata taatcagtaa atgttatact tttcatgacg ataaaaacat
    21481 tttagtcata agggggttat gagtttgcca cgatttacgg aacaagaaaa agaaatcata
    21541 aacagtaaac ttttgataga gggagaaaag ctgttttcat tatacggctt aaaaaaagta
    21601 acagttgatg atttagcagc tgctgtaaac atatcaaaag gttcattcta tgctttttat
    21661 cccagtaaag aacatctata tgtggaaatc aattttcgct tacaaaaaga gttatttaca
    21721 agtatagaaa caactgttaa aaagaaaaag tataagaacc atagagattt agcaaaggac
    21781 gttattatgc taagtttaac gggggtgatt acttcgccta ttctttcaca gattgattta
    21841 tctttaatgg actatctgca aagaaaaata tcgtcagata tttttgaaaa tcatatgtac
    21901 agtgatattc gtatattgga gatactggaa ggtttagggg ttgtattttt ggttccgcat
    21961 acggttatca taaaatccct gtattctgtt ttatcctgtt tggaacaatt caaagaggac
    22021 gaagaattga atatggtaca aaatctttta gtgaatggta ttattcagca agtagtagca
    22081 gagtaataat tttgtttggt tatttgacag gcaagtggcc aaacaaattt tttacagttt
    22141 atatgactat atatctataa acgtcataaa gttaaaggag gaaagaataa tgattgatgt
    22201 aaaaaaactg tattttagtt atactgacaa accgtttgtt gagaatgtaa gtttccatgt
    22261 tgggagagga gaaattttcg gttttttagg accctctggt gctggaaaat caactattca
    22321 aaaaatttta actgggctta atacaagtta taaaggcagc gtaaaagttg caggtacaga
    22381 aataagggag catacaaacc ggttttatga aaatattgga gtagactttg aattttcaac
    22441 ctgctacgag aaattcacag cacgacaaaa tctcgcctat tttgcttctc tatatgacaa
    22501 gcaacctcgg tctattgatg aattattgca tatggtaggc ttagaaaatg atggggacaa
    22561 aaaagttgct gacttttcca aaggtatgcg ttctcgcttg aatttcatta aagcgttagt
    22621 acatgaccct gatatattat tccttgatga acctacaagc ggtcttgacc ctacaaataa
    22681 tcgtttaatg aaagatatta ttcttgcgga aaagaaacgc ggaaaaacga ttatcattac
    22741 tactcacaat atgtatgacg caacagagct atgtgaccaa gtagccttta ttgttgccgg
    22801 aaaagtcagt gcattggata gtccacataa tttgattatg tcaaggggag ctgccaaaat
    22861 acactatacc tactatgata aaggcgagaa aaccggagaa tgtctgcttg atagaaccac
    22921 agaagataag ttgttaaaaa gtttaatttc cgaaaaccgt ctgctttcta ttcatagtag
    22981 tgagcctaca ttaaatgata tttttgttga tattacaggg aggacacttc aatgagattg
    23041 ggacgtttaa tttgtggtga tatacatttc caatggaaat atggtttcta ttttatctac
    23101 tttatattaa cagttttata tgtgtgtggg attgccgctt tgacgggaca ttggaaaacg
    23161 gatattgctt ctatcatgat atactctgac ccggcagcta tgggattgtt ttttatgggg
    23221 gcgattgtcc ttttagaaaa aagccaaaag gtattaaatg ctatggtagt atcgcctgta
    23281 aaagtatcgg agtatatact ttcaaaaacc atcgcattga ttgctatttc tacaatcatt
    23341 gcggtgatat tgggatttgt ttccggcagc aatcatttac ttggtatcgc agttggtaca
    23401 gctttgacat cagcaatctt tactatgctt ggcattatcg cggctacaaa aatttcaaac
    23461 ttgaaccaat ttctcattgt gattatgccg atagaaattg tttgctttgt tccacctatc
    23521 gttggtctgt ttgtaaagtt accagatata ttccgctttt ttccgttcac agcttgcatg
    23581 aacctcataa ctggaaaaag cattttacta tcgtttgata tggtacttgt aattgctacg
    23641 ttgattatcc tctacatagt tgcgcgacat acagttaagc atatgtggag aagtttggga
    23701 ggtgtaaagc tatgaaaagg atattatcca gtaccataca ggtatttaag caaatcaaga
    23761 gcgaccctat gatgtttgca gcttgtttta cgccttttgt catgggagct ttgattaaat
    23821 tcggtattcc ctttcttgat ggaataacag atttttcttt gcaaacatac tatccgatct
    23881 ttgatttatt actttctatc atggctcctg tattgctctg ctttgcattt gcaatgatta
    23941 cattagagga aatcgacgat aaagtgtcac ggtacttttc gattacccct cttggtaagt
    24001 cggggtatct ctttacaagg ttgggagtac cctcaattat ttcggcggtc atcgctttta
    24061 ttgtactgtt gcttttttca ttagaaaagt tgtccattgg aatgacaata tgtttggcgc
    24121 ttctcggctc agtacaggca attattgttt cgcttatgat tataacatta tcaagcaata
    24181 aattagaggg tatggcagtt acaaaactag ctgcacttac attactcgga ataccagctc
    24241 ccttttttat tgatagttac taccagttcg cggttggctt ccttccatca ttttgggtag
    24301 cgaaagccat gcagaatgaa gcggttcttt atttctccat aggattgatg gtagctttgg
    24361 tctggtacta cttccttata aaacgtctgt ttaggaagct ggcaggataa gaaggcatat
    24421 tatggaaaaa ggtagacttc tgcaaaacga aaatataaat aaggctattc aggtttccaa
    24481 aatcacacta atcatcaacc tgggtttatc gctacttaaa tttgctgccg gatatatagg
    24541 aaagtcaagt gccatgctgt cggacgctgt acactcggct tctgatgtat taagcacaat
    24601 cgttgtgatg gttggaatta aaatatcaga aaaacagcct gataaagaac atccttatgg
    24661 gcatgaacgt atggaatgtg tggcttccat tattctctct gttgcattgg ctataacggg
    24721 tgctggaata ggatattcag gtatcaagaa aatcttttca ggacagtaca atacgctttc
    24781 tgtctctagt ggaatcgcac ttacggcggc agtcttatcc attgttataa aagagtggat
    24841 gtactggtat acaagaagtg ctgcaaagca tacaaattct gacgcactta tggctgacgc
    24901 atggcatcac cgctctgatg cactctcgtc ggttggttcc ttaattggca ttttaggagc
    24961 acgtttaggc tatgctattc tcgaccctat tgcaagcatt gtgatttgcg gttgtatctt
    25021 aaaagctgct ttggacattt tcaaagaaag cattaacaaa atggtagacc atagttgtga
    25081 caatgctact gaaacgaaga ttagggaagt tgtgcttcag cagcaagggg ttgatggtat
    25141 tgatgaatta aaaacccgca tgtttggagc gaaaatgtat gtagatattg aaatattagc
    25201 tgatggtaat ttagctcttt atgacgctca tagaattgct gagggagtcc atcaaacgat
    25261 tgaaaacaac tttccacaat gtaaacattg tatggtacat gttaatacga atgaacttcc
    25321 ctattctcca taggacaggg aagatgattt aagactattg aaagggaaag gaggggaagt
    25381 aggttatgga acaatactgg tggattatca ttgttatggt tttgattgtc gttggcttac
    25441 ttaaaattgt aattatgaag aagtataacc aaaggagaaa ttctaacaat cgttcaaaaa
    25501 atagcgagga tgacaagatg tattaagagc aattatccga tggtatgaga tatataccta
    25561 tgaaatttga acgataaatc ccagtttgta aaagtcaata aatccaaaga taaatttgta
    25621 tttgaaagga gttttgaaga atgaaaaaat atgagtatgt agaaattaat tataatgcga
    25681 aaggagttgt attcctttgt actgataaac acaaagaaat tattgacagt tatgcaaaaa
    25741 cggggtatca ctacgcaggg tttgtaccca cagaaattga cgcaaaaggc tgtatgcgaa
    25801 agatagattt aatttttgaa aaagaagatt aacttatttt agggagttgg ttctaatgat
    25861 aggacttaaa agacgagata tgaattatat tatgaaagtt ggtgctaaag ctcaaggtgt
    25921 ttctgtatct gaatttaggg ctatgctaca agataccata gacaaaacta tggaaagtac
    25981 agacccagac gtgcaggcaa attttaaacg atactttgga aataagcggc caacaccaga
    26041 ggaatatatt tatacgatta ccaaaaagac aaaggttaag gtttaaatgt tgtaaagggg
    26101 gcttgccgta tgaaacgctt taaatacaat tcagtatatt caagcccaat ttaacagcat
    26161 actatttaca attaaatacg ctataccagt agctcgtaca aaatttatat tttgtgcggg
    26221 cttttttaat acccgaaaag ttttttaatt attttcattt ttttggaagc aaatcacacc
    26281 ttgctgtaca gatatggtgc ggaaagaggg ataaacactt ttcaattcat agctggaaag
    26341 gaggtgagat tatgaaaccg tctgacttcc aaaaaacaat tcagtgtcag tttgaatgta
    26401 agctgaaaaa agttgtcaaa ggtattatct ggaactaccg caaagaatta agacgaagaa
    26461 aaaacaagga aatatctttt agcgaattac cagacattac ggtagaaagt ctggcaacat
    26521 gggacgacta cgaaacaaac tacacaattt ttaatgtgtg tggcatagat attaaagtcc
    26581 ttgatgatga acttgcagaa gctctcaaac agctacccga aaaaaacgta acaatgtatt
    26641 gatgtattac ttcttggaaa tgagtgatac agagattgca gatattatga attataccag
    26701 agaggctgtg ttcaaacaca gacaccgtgc actagaaact atgaaagaga tactaaatga
    26761 aaagacaaga cagtaaagga cgcaatttgc gtcttggaga gagccagaga aaagacggaa
    26821 gatacgttta taaatatacc gatatattcg ggaagccaca gtttgtatat tcttggaagc
    26881 ttgtacccac agacaagaca cctgctggaa aacgtgatga tatatcgttg agggaaaaag
    26941 aaaagcagat aaaaaaagac cttgacgacg gtatcgacac ggtgggcggt aaaatgacag
    27001 tctgccagct ttacgaaaaa aagaacaatc aaagaaagaa catcaagaga aacacggaat
    27061 taggacgaca gtatcttgtg aacgcactgg aaaatgaccc attgggtatg agggcgattg
    27121 atacggtcaa gcagtccgac gctaaagaat gggctatcag aatgaatggc aaaggttatt
    27181 cttacaagac gatagataac tataagcgtt ccctaaaggc ttcattctat atggcgatac
    27241 aagacgactg tatcagaaaa aatcctttta attccaagtt gagtgatgtt ctggaagatg
    27301 atacggaagc aaaggttatc ctgacaccag agcaggaaga aaaactcctt gcctttatgg
    27361 aaacagataa tgtttacagc aagtatcgtg atgaagttat tcttctgctg gaaacgggac
    27421 ttcgtatctc tgaattatgc ggactgacaa cacacattga tatgcttaac cgtgtgatta
    27481 acatagacca ccagctattg cgtgatacag aggtgggcta ctatatttcc aaagctaaga
    27541 caaaaaacgg taagcgagag cttccattaa cagaaagagc atatcaagca ctcaagcgaa
    27601 ttttaaagaa ccgaggaaaa gcacaaccgc ttgttgttgg tggttatagc aatttcttat
    27661 tcttgaaccg tgagggattg ccaaaggtag caggaaatta tgaaagtatg gtgcgtgggt
    27721 tgattaagaa gtacaacaag acccataagg acaagttacc aaacatcacg ccccattcat
    27781 tcagacatac ttactgtacg aacatggcaa ataagggaat gaacccaaac accctgcaat
    27841 acatcatggg acacgcaaac ataaccatga cattagggta ttatgctcac ggtactttcc
    27901 agtctgcaaa ggcagagtta gaaaggctgg cttcttaata tcgaaaatgt atcttactac
    27961 ttatttacta cttttggttg cattttcatg ctggtaaatg ccagcttatg caaggtatct
    28021 tccaaaaaga aaatgcccat acagcccgaa aatgcgggat atatcggcat ctaccaactt
    28081 atgaaaagat aatcagaaat ttagtaagtt tttataataa aaaaataaga ggctgtccaa
    28141 aaatagagat aagccttttt tcataatcgg aattataaaa atacacttga tttcatatga
    28201 aaaagagtgt ctcataaact aaaaagttta ctttgagaca ctaccttttt tattcttatt
    28261 tatactttgt agacatatta taatatgcgt ttcactggta tctgaacttc tgtcaaccag
    28321 tcttcttcac tctctttatt ccatatacca tctatataag attctctagg atttccaacc
    28381 actttatatt tattgtcctc tatccattta aatgcatctg cataagtttt tcctaatgtt
    28441 gaatatggac cattgtgtaa tatacaaact gcattttcaa ctgcatctaa ttctttaaat
    28501 tttaatatat ccgtatcttc ctttaaatca tttatagctt gacacatttc aacatccata
    28561 tcagtctctc tatattcacc atcatgatat atattaaaac aataatcagg aactgcacat
    28621 gtacaccctg ttttcatcat ttcttcagcc attacatttg gaaatagatt aaaaagttca
    28681 tcatatccct ttaacacttt tctcatagaa gcaactttaa cttttggtaa agacttaata
    28741 attacatcat tcatattgac ttctcctttc aaattcataa ggtaattacg tagtttaatt
    28801 attttatatt cttcactttt aattacatcc aacatttctt tttctttatt cattaaaata
    28861 ttttcaactt cttcaggatt ttctacagct ttttttatct cagctaatga taaacccatt
    28921 tgttttaaag ttattatctg atgtaattta ggtaattgct ctgttgtata atacctatat
    28981 tttgtaaatt catctacata ttctggtttt aacaacccta tatcttcata ataccttaat
    29041 gtctttgtag ttattttatt tattttagaa aacattccta tagaaaacat ctcatcacct
    29101 gcgcattaga tttgattaat ttaagtttaa actttcccct taggttaaag tcaatacaaa
    29161 ataaattata attgtattaa ttcttcaatt ctatctccac tcaataccaa aatatcccct
    29221 gtttttataa tggttgaacc tttaggtatt aaaacctgtt cctttctttt tatcataact
    29281 attaatatat cgtctggtat attagaatct attatactct tattagccca tttattaccc
    29341 tcatcaacat ttacttcgat aagtttagag ctaatttccc catcataatc attaaatgtt
    29401 tttaatacag gtgtattatc atcaactaaa tccaactttc tggctacagt tggtatcaat
    29461 gaaccttgta ttcctactga aagcaacgct ataaaaaata ttatatggaa tatgtcattc
    29521 tctatggcta ctccttgagt aacagcataa attgcaaaaa ctactgaagc cgcacctcta
    29581 agacctaccc acgatatgaa taatttttct ttatttgaga atttaaattt tgctagaact
    29641 ataaatgttg ctaatggtct tgcaataact atcatgaata tagatatggc cattgctata
    29701 ccaaatacat ttggtaactt agaagggaaa gataacaatc ctaacataaa gaataacatt
    29761 atttgcataa tccatgaaat tccatcaaag aaatgaacca aacttttttt aagaggtatc
    29821 ctactatttc ctataataat acctgaaatg taaacactta aatatccatt tcctccaaga
    29881 tactcgctaa atgcataaga aagtattgca atagctgtta taaatatagt ataaaatcct
    29941 tctatctcga agtttgaatg ccttaaaata taaacagata attttgctag tataaatcct
    30001 atagctaacc caaaagctat ttgttttatt accattggtg caataagttc taccccagaa
    30061 ttattcatca atgtcaaaag aattagtgtc aacatatacg ctattgggtc attacttcca
    30121 ctttcaactt ctaataatga tgcaagtgaa ccccttaagt ttagtttttg tgaacgcaac
    30181 acagcaaaca cagatgcagc atctgttgaa gctgcaattg ccccaattaa taaaccttca
    30241 agtaaactag ttcctattac catgtggcaa aacatacctg taaccccaga cgtaattatt
    30301 acacccagtg aagacattaa tatagaaggt atagcaacag gtttagccat attccagttt
    30361 gttccaaacc ctccaaaaaa cattataaat attagtccaa tagaacataa ttgttttgtt
    30421 aattcatagt tctgaacctc ccatgactaa agtcacagag ttcctgcttc atagaatttt
    30481 gcatactaaa atatgtctta ctaattctcc acaggc
//
LOCUS       DAGQWR010000037        30496 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.037, whole genome shotgun sequence.
ACCESSION   DAGQWR010000037 DAGQWR010000000
VERSION     DAGQWR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.037"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            86..1144
                     /locus_tag="MIW95_001432"
     CDS             86..1144
                     /locus_tag="MIW95_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBY3150763.1"
                     /translation="MIVCNNPGTWMRMYPQLRHAVWHGVTLADFAFPFFVISLGVTIP
                     ISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNPDLNSVRILGVLQRMGLVY
                     FVTSLVYLLLKKLNVGSTATIITFLCIATFIIVGYYILAKPYGFELEGSLAQLVDLHF
                     FKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENIGEYKKFFKILVMSIILLI
                     GAFIFNQYFPFNKRLWSSSFVLLMAGSYGMLLSIFYFICDIKNKSKIFTPIIALGSSP
                     IFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELITPWAGTTWDSLIFSLLYVL
                     FWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="MIW95_001433"
     CDS             1398..3422
                     /locus_tag="MIW95_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY3150764.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAGT
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="MIW95_001434"
     CDS             3642..4328
                     /locus_tag="MIW95_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBY3150765.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="MIW95_001435"
     CDS             4423..5478
                     /locus_tag="MIW95_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBY3150766.1"
                     /translation="MIKKISTILSLVLLISISSTIEVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGIKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTAKLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7152
                     /gene="murJ"
                     /locus_tag="MIW95_001436"
     CDS             5593..7152
                     /gene="murJ"
                     /locus_tag="MIW95_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBY3150767.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDDKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRRR"
     gene            7194..8900
                     /locus_tag="MIW95_001437"
     CDS             7194..8900
                     /locus_tag="MIW95_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBY3150768.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNDTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8922..9995
                     /locus_tag="MIW95_001438"
     CDS             8922..9995
                     /locus_tag="MIW95_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBY3150769.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEYISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10007..10729
                     /locus_tag="MIW95_001439"
     CDS             10007..10729
                     /locus_tag="MIW95_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBY3150770.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10745..11902
                     /locus_tag="MIW95_001440"
     CDS             10745..11902
                     /locus_tag="MIW95_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBY3150771.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNIDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTIFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11905..12744
                     /locus_tag="MIW95_001441"
     CDS             11905..12744
                     /locus_tag="MIW95_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY3150772.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNEDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYTFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12759..13934
                     /locus_tag="MIW95_001442"
     CDS             12759..13934
                     /locus_tag="MIW95_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBY3150773.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13963..14712
                     /locus_tag="MIW95_001443"
     CDS             13963..14712
                     /locus_tag="MIW95_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY3150774.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYKRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14734..15642
                     /locus_tag="MIW95_001444"
     CDS             14734..15642
                     /locus_tag="MIW95_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY3150775.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15690..16442
                     /locus_tag="MIW95_001445"
     CDS             15690..16442
                     /locus_tag="MIW95_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY3150776.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16420..17763
                     /locus_tag="MIW95_001446"
     CDS             16420..17763
                     /locus_tag="MIW95_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBY3150777.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNNVK
                     VELASNPEFLSQGTAVKDTLYASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            17769..18860
                     /locus_tag="MIW95_001447"
     CDS             17769..18860
                     /locus_tag="MIW95_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBY3150778.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18944..20857
                     /locus_tag="MIW95_001448"
     CDS             18944..20857
                     /locus_tag="MIW95_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBY3150779.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNSELKKVAVAIIDDDKDKIGRIIHNVKIVGTTSDIKAVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21056..21763
                     /locus_tag="MIW95_001449"
     CDS             21056..21763
                     /locus_tag="MIW95_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBY3150780.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22065..24176
                     /locus_tag="MIW95_001450"
     CDS             22065..24176
                     /locus_tag="MIW95_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBY3150781.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTAL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24374..25339)
                     /locus_tag="MIW95_001451"
     CDS             complement(24374..25339)
                     /locus_tag="MIW95_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBY3150782.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25624..26580)
                     /locus_tag="MIW95_001452"
     CDS             complement(25624..26580)
                     /locus_tag="MIW95_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBY3150783.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVDIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26948..27736
                     /locus_tag="MIW95_001453"
     CDS             26948..27736
                     /locus_tag="MIW95_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBY3150784.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVDDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27859..28329)
                     /locus_tag="MIW95_001454"
     CDS             complement(27859..28329)
                     /locus_tag="MIW95_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBY3150785.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28468..29112
                     /gene="uppS"
                     /locus_tag="MIW95_001455"
     CDS             28468..29112
                     /gene="uppS"
                     /locus_tag="MIW95_001455"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBY3150786.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29155..30060
                     /locus_tag="MIW95_001456"
     CDS             29155..30060
                     /locus_tag="MIW95_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY3150787.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQKVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT        12091 a         3311 c         5405 g         9689 t
ORIGIN      
        1 aatttcaaat aatgttgtag attctaaact tacgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcgg attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaaactaaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttaaattctg tgagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagtat atttactact
      421 aaaaaaatta aatgttggaa gtactgctac aataattact tttctatgta tagctacatt
      481 tataatagtt gggtactaca tattagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa gggacatttg tacaagccag aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaaa acataggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatgt tactgtcaat
      841 attttatttt atatgtgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cctattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatatacg gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagattta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaac gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcgccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgatgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 aattaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt atctcaaatt aaatctgctg taaatgtaaa tgtaactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agactctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gactcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aagacttatg
     2701 tttctgcata ccatagcaca aacttatcag aaaaaacagc aggaacagta tatcaaattg
     2761 gtgatgggat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcgggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gacaaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 acactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataac aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaataaaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacgg ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattaa gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggat atgggcttaa
     4081 taggccctag accagagaga ccgaacttta ctgttcaatt caatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagca caatagaggt atttgcagat
     4501 gcaaatccca aaagagaatt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 ataaaagtag aaagaataag aggaaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaatgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gtaagaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agaatttatt gcagagattg attctaactt gaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctaaattag actttggtag cagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttaatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattgggct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctaagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaacgt atggatacta ccagcaggag cactattggc tatggcgagt
     6181 caactattat ttcaactgcc atttgcattt aagaagtcct acaagtataa gccatatata
     6241 aacttaaagg atgagtctat aaaagaatta gtaaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gacagattat tggcaactac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aagctagcaa aaatgtctgg taaagatgat
     6481 aaagaaggat ttataagtac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttaggatatt atttcaaagg
     6601 ggtaaatttg atgcgttatc aacagatctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacgc caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattagg tcatgcaggt attgctatat caactagtac atccaatata
     6841 attacagtta tattgttatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggactt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagaagat aattaccttg aaattttaaa ttagagtcag aaaggatgaa aatatggatt
     7201 ataagaataa ttatgagatg tggttaaatt ctccttattt tgatgagcaa acaaaaaatg
     7261 agctattaag cataaaagat gatgaaaaag aaatacaaga tagattctat aagaatttag
     7321 agtttggaac aggtggttta agaggtataa taggagcggg aactaataga atcaatattt
     7381 atactgttag acgtgcgaca ctgggcgttt taaattatat catgaagact caaggagaag
     7441 aaggtaaaca aaagggaata gttatagcac atgatagtag atacatgtct agggagtttt
     7501 gtatagaagt agcaaagact ttaagtgctt atggtgtaaa ggcttatatt tttgaagaat
     7561 taaagcctac accagaactt tcttttgctg ttagatattt aaagtgtgct atgggaatag
     7621 taataacagc aagtcataat cctaaagaat acaatggata taaagtatat gactcagatg
     7681 gtggtcaaat ctgtatagat atggcaaatg atataatagc agaagttaat aagattgatg
     7741 attacagcac tattaaaagt atagattttg aagaagcttt atctaaaaat ttaataacta
     7801 tattagataa tgaagtagat gatgagttta taaaagctgt taagaagcaa gttttaagac
     7861 aaaatataat agatgaatac gggaaaaaat taaaaataat atatacacct attcatggta
     7921 caggaaataa accagtaaga aaagtcttaa atgaatgtgg ttttgaaaat gtaatggtag
     7981 ttaaggagca agaattacca gattctaatt tttcaacagt aaagtatcct aatcctgaag
     8041 aaaagtcagt gtttaatata gctattgaaa tggcaaagaa taatgacact gatttgataa
     8101 taggaacaga ccctgattgt gatagagtag gtatcgttgt aaaagattca agtggtgaat
     8161 atgtagtctt aaatggaaat caagtcggtt cacttttagt tagatatata ttagagagtt
     8221 tagttgaaga aaataaactt cctaaaaaca atcctacaat aataaaaact atagttacat
     8281 cagaactagg tgcaaaaata gcaaaggctt ataatgtaga ttgtttaaat actttaacag
     8341 gatttaaatt tataggagaa aaaataaaag cgtttgaaga aagcaatgat agaagtttta
     8401 taatgggata tgaggaaagc tatggttact taattggaac tcatgctaga gataaagatg
     8461 gtgttgtatc ttcacttatg atatgtgaaa tggcagcata ctattcttca aaaggtatga
     8521 acttgtatga agctttgata gatacatata ataaatttgg atactacaaa gaggacttaa
     8581 agtcagtaac tttaaaaggt atagatggaa taaaaaaaat aaaagagatg atgctttact
     8641 ttagaagtgt taaaatagat aatgtggctg atgtaaaagt agataaaatt ttagactata
     8701 aagatggtgt tgatgattta cctaagtcag atgtacttaa atttttactt gaagatggct
     8761 cttggatagc tataagacct tcaggtacag aacctaaaat taaattttac tttggagcaa
     8821 attcagacaa tcaagaagat gtagaattta agttgaacaa tttaatatct tatatattaa
     8881 atgtagtgga ttctatataa aaagctaagg ggagataaat aatgaaagta tacaatgtca
     8941 taatggctgg aggaggagga actcgtttct ggccacttag tagacaagaa gtacctaaac
     9001 agcttataaa tttaagtgga gaagatgctt taataaacga aactataaac agaatagatt
     9061 ctctagcaaa aaaagatgat ttatttatag taaccaatga gaagcaattg gaagctttaa
     9121 aggatatagt aaaagataaa tgtttggata gtaatatatt gccagagcca tgtgccagaa
     9181 atacagcagc agctataggc tttgcagcat ttaacataat gaaaaaatat ggtgatggtg
     9241 ttatgtgtgt atatccagct gaccattata taaaagatga aaaagaattt aaatctattt
     9301 tggaaaaagc tatctatata gcagaaaata atgataaact agtaactatt gggataactc
     9361 ctacatttcc atcaacagga tatggataca taaattttaa tagagaaaat acgatagaag
     9421 atgttgcata tgaggtagtg gagtttgtag aaaaaccaaa ctatgaaatt gctaaagaat
     9481 atgtaaactc taaaaagtat gtatggaata gtggaatgtt cgtatggaag gtatctaaaa
     9541 tattagaaga ttttaagaga tacttgccaa aagtatatga aaaattagaa tatataagta
     9601 aatacttagg aacaaaagaa gaaatggaaa aaataaaaga aatataccca actatacaat
     9661 caatctctat agattatggt attatggaaa gaagtaatga tgttatagta gttcctggtg
     9721 attttggatg gaatgatgtt ggttcttggg attctctagg tgcaatatat ccaactgatg
     9781 atgaaggtaa catcaaaaga ggtgaaaata ttactataga tactaaaaat tcaattatat
     9841 attcagatga caaattaata tcaacaatag gtattagtga tttgatagta gtatctacga
     9901 atgatgcagt tatggtttgt agaaaagata aagctcaaga tgtcaaaaaa atagtagaac
     9961 agttaaaaga agaagataga caagaatata tgtaggaggt gttcatttga ttcctaaaaa
    10021 aatacattat gtatggtttg gtggtccaaa aggaaatata gaaaatatat gtataaattc
    10081 ttggaaagaa aagcttccag aatatgagat agtagagtgg aatgaaaaaa actttgatat
    10141 agagaaagaa ataaaaggaa ataagttttt ggaagaatgt tataaaagaa aactttgggc
    10201 attcatatct gattatacaa gaataaaagt actctatgaa caaggtggag tttatatgga
    10261 tacagatatg caaatattaa aggatataac tcctctttta gaaaataata ggctaatctg
    10321 tgggtatgaa gatgatagag agtatataaa tggtgctata ataggtgtag aaaaaggaca
    10381 tcctttttta aaagacttat tggagtatta tgaaaaagaa gtacttactt cttcattgtt
    10441 tacaatacca aaaataatga ctcatcttat ggaaaagaat tataagaaga ttgacccaaa
    10501 taattatgaa gaaggaatac gtgtttacga caaagaatat ttttatcctt ttggatttaa
    10561 agaagacttt acaccagaat gtataacaga aaacactttt gggatacatt ggtggggaaa
    10621 aagttgggct aaaaagagaa attatttctt agaaagcaaa cacttaacag gtgtaaataa
    10681 gatatggaag tgttgtaaaa tatttgcaag taatacttta cgaagctaaa agggagaaag
    10741 taaaatgaag actttaaatg atgatagaaa tattatgatt agaaaatatc tttatatatt
    10801 tgttttgttt atcatattac ttagaaagta tatgatatca tttattgacc caaatataga
    10861 tatagggatg attaaatcag cgttatttta ttcttcaata ggaatactaa tgctactttt
    10921 tttatttgat aagagaaaaa gtataacaga aatggtacta gttggagttt gtgtcttact
    10981 ttatttatta aatagggaag gagctatatt attaatcgtt ttattagctg tatcagctaa
    11041 gcaaatagat gataaattta tagttaaaaa ttatttaata atatcggcat gttttttaat
    11101 ggtagctata ttattattta atttatttcc ttcattaata tttaatcagg aagtccacta
    11161 tagatatata gaaaaaatag atatgcttgt tacaagaatg gattttggac ttggtaatcc
    11221 aaatagtgta ttttatcata tggttactat atatgcagca tatatattct taagatataa
    11281 agattataat aaatgggata gaataatttt atttggttct gcattttttg tttatcaaac
    11341 cacatatagt agaactggat ttttcactat attagcagga ttaatatttg tagaaataat
    11401 aagatggata gatataaaaa aaataaaagg actgccaatg ctcctaaaga cattaccaat
    11461 aatacttact ctatttagtg taattattgg tacaatattt gataaaagca ctttattaaa
    11521 tagattatta gcaagtagac ctaagttctg gcatgtatac ttggctgaaa aggggaattt
    11581 tctaaaacct tttggaaatt catatagtcc tgctataaaa gcaactaatc cattagatag
    11641 ttcttatgta tatataatta gtgtactagg agtagttgct tgtatattat ttatgtattt
    11701 gatgtataaa ggaatagaaa gctttataga aaaagataaa aaagcatatt tagtagcttt
    11761 atttatattt ttactatact cttttgcaga aaatttatta ttagaagctt catttagttt
    11821 tgcagtagta ttactaataa aagaagtgat attaaatgat aaaagggaaa tagatttgtg
    11881 gaagatgaaa agtaggaggt agtaatgtta atatcattaa taatgccaac attaaataga
    11941 tatgatgata tatacttact tatggatagc ttagaaaatc aaacttacaa aaactttgaa
    12001 cttatagtag tagaccaaaa tgataatagc aaagttaaag aaatagttga taaatacatt
    12061 gataagttag atataaagta tataaaaagc tctaaaaaag gattgagtta taacagaaat
    12121 gtaggcatag ataatgctgt agggcaaatt ataggtttcc ctgatgatga ttgtgtctac
    12181 gaaaatgata cattagaaaa agttataaat ttctttaata aaaatgaaga ttataaaata
    12241 tacagttgca aaactatgga ttcaaataaa gttgatgcat ttaagaaaat gtatgatgga
    12301 acttgtgata tcacaagttc caacgttttg gatactataa catccataac tttttttatt
    12361 gattttgaag gtaaagatta tacaagattt gatgaaaaat tgggtgttgg tggagagttt
    12421 ggtgctggtg aagagataga ttatgtttta aacttattga gtttaggttt taaaggaaaa
    12481 tactttggaa atgatataat ttaccatcct gcaaaaaaac attctaaatc taaagaaaag
    12541 tatcaaaaag attataatta tggaagaggc tttggagcgc tttgcaaaaa ggaaatagtc
    12601 tatagaaaaa attatacgtt tgcaaaagtt atggtatcaa aacttgtaag aaatataggg
    12661 ggtcttatat tgagttccaa tagagattat catagtgcca ctataaaagg aagaattaat
    12721 ggatttagac agtacaagtt ataatgagga tggtgtttat ggacaaggtt aaatttttaa
    12781 taaatatgct tttggctttt tttatatatc catttaataa gcataagttt aatggtagag
    12841 atatctggct tataggtgga cattcagggg atatatacaa tgataattca aagttttttt
    12901 atgagtatat gctaaaagaa cataatgatg tagaaactta ttgggtagta aataaagaca
    12961 gtaaagtatt tgataaaatt ccaggcaaaa aattaataag aggaagtgta gaaaactatc
    13021 tgtattatta caattcaaaa gctatagtat tttcacatgc accatcagca gatatagcac
    13081 catacaattt tgctgtacct gttcttaatt attttcataa aaaaacgata aaagtgtttt
    13141 taaatcatgg gactataagc tttaaaaaaa gaaaacctat gaacaaaaag ttcaaaaata
    13201 ttatagataa cctatataaa agctataata tagttacagc aagttctgaa tttgaaagaa
    13261 atgttatggt aaatgattgg ggaatgttag atgactcagt ttatataatt gggaatgcaa
    13321 gatatgacaa tttaccaaca aatgaagttg cacaaactcg agatatatta tatactccta
    13381 catggagaga ttggataaaa tttagttcag gaaaatttac ggatacagat tattttaaaa
    13441 atataatgaa ttttttaaat gatgacaaat taaataaaat attagatgag aaagatataa
    13501 atgtaaaaat ttatatgcat catcttatgc atgagtttat agatgatata aaagaaaata
    13561 taacaggaaa acgaattgtg tttttagata aaggggtaac tctagcaaat gaaatcagaa
    13621 aatcagctgc aaatataact gattattcaa gtgtagccat agactttttg tatatgaata
    13681 gacctatctt gttttaccaa ttcgatttgg atgaatatat ggaaaaagtt gattcatata
    13741 tagatttaaa aagtgaaatg tttgggtctt tggcttataa taatgatgaa gccgtgaata
    13801 aacttattga tattattgaa aataattttg aggttatgga taaccaaaaa aatgaaagaa
    13861 ataagttttt cagatataat gataataaga actgcaagag gatatatgat tgtgtattaa
    13921 gtaaaattaa ataaatattt tataaatgat gaggaaggta atatgaagaa gaatttagta
    13981 tctataatta ctcccatgta taattctgaa aaatttattg aagcaaccat aaaatcagta
    14041 ttaaaccaaa cttatcaaga atgggaaatg ttaattattg atgattgctc aacagataat
    14101 agtcctaata tagtcaaatc ttatatgcaa caggatagta gaataaaatg tataaagact
    14161 gagactaata agggtgtctc taatgctaga aatttagcac taagtaaggc aacaggacaa
    14221 tttatagctt ttttagatag tgatgaccaa tggaatagca gtaagttaga aaaacaagta
    14281 aattttatgt tagaaaatga ctatgtaatt tcatttactt catatgaact gatggatgaa
    14341 aatgataaaa aattaaacaa agtaataaaa gttccaccta atgtagatta taaaagatta
    14401 ttaaaaggaa atatcttagg atgtcttact gttgtgattg ataagtctaa attggatttt
    14461 gaaataagaa tgagtggtgt aagacatgaa gactatgttt tatggttatc gatattgaaa
    14521 aaagggcata ttgctcatgg aataaatgaa gttctagcat tatatagaaa atctagcaat
    14581 tccctaagtg gtaataaaat aaaagctgct atgtggactt ggaatatata tagaaatata
    14641 gaaaaaatac ctttatacaa agctatatac tactttatta attatggaat aaacggaatc
    14701 aaaaaaagtt agacagagag gaagatttaa ttaatgtata aatataaatt tactgtattt
    14761 actccgacgt ataacagagc tcatttactt gaaaatttgt acaatgattt aaaagctcaa
    14821 acttatgatt tcaatgattt tgaatggttg attgttgatg atggaagtag cgacagtact
    14881 aaagagttag ttgaaaaatt tatatcagaa aataaattaa atataagata tatatacaaa
    14941 gaaaatggag gaaaacatac tgcaataaat ctaggtgtaa aaaatgcaga tggagagcta
    15001 tttttcattg tagatagtga tgatggttta attaaagatt ctcttgaaat tgctaatgat
    15061 gagtgggatt ctttagagaa taaagaagga tttagtggag ttgttggact ttgtgtatat
    15121 ccaagtggag atttgatagg gacagaaatg cctgaagata agaaaatatg tcactttgca
    15181 gacctatatt ttaagtatgg cgttaaggga gataaaagca tagtatttgt aactgatgag
    15241 cttataaaat ttccttttcc agaaagaaaa gaagtgagat ttttaccaga aagtgtagta
    15301 tggaatgaaa tgtctaaata ctataaagtt atatgtgtaa ataaacctat gataataaga
    15361 gattatttgg atgatggttt gactaagaat atattaagta aaaatgcatt aagaggtaga
    15421 gcattagagt ttttatattt aataaatcaa aatacatatc cattaagtag atatccatat
    15481 atgtggataa aaaattacat aaatttagcc agatactcat tgttgtctga tagtcattat
    15541 tttggtgaat taagaaaagt atctgataaa ttgttgtatt tagcattatt cccattagga
    15601 tattacaaat atattggaca aagaaaatta gtatctaagt aaacttgttg gtatagtact
    15661 gctagttgct atataaggag agtaaaaata tgaatgagcc tttagtatcg ataattactc
    15721 ctgtttataa ttcagaagag tttttatcag agacgataaa gtcaatccaa aatcaaactt
    15781 ataaaaattg gcagctttta ttagtggatg attgttctaa ggacaattca agtgaaatta
    15841 taaaaagttt tcgaaaagaa gatgctagaa taaaatatat aaagcttgaa aaaaattctg
    15901 gagctgcagt atctagaaat gttggtatta agaatgcgga aggtcgattt attgcatttg
    15961 tagatagtga tgatttatgg gatagtagaa aattagagat tcagatagaa tatatgttaa
    16021 aagaaaatgt aggtttttct tttacatctt atagatatat gagacaagat gggtcaaaaa
    16081 cgaataaggt tgcaagagct cctaagaaaa ttgactatga aggattactt agaaatacta
    16141 taataggatg ttctacagtg gtcattgata aggagattgt aggagagttc agcatgcctt
    16201 tggtaagaag aggacaagat actgcgacct ggttacagtt acttaaaaag gaaaaatatg
    16261 catatgggat tcaggaggat ttagtgaatt acagattagt tggaaattct atatcaagta
    16321 acaagataaa agcattaaaa cgaacttgga atacttatag aaatgttgaa aatttaagtt
    16381 taccaaaaag tctatatgta ttttgtttct atgtttttaa tgccattaaa aaaagagtat
    16441 aagttttata tacttggagg taaaaaaatg aaaatagcta tagctgggac gggatatgta
    16501 ggtttggtta caggtgtttg tctagcagag gtaggcaatg aaaatgtagt ctgtgttgat
    16561 gtagatgaac aaaaagttaa atcaatgaaa cttggaatag cacctatata tgaagaaggc
    16621 ctagaagaac ttatggttaa aaattacaac agaggaagaa tagattacac tacagactat
    16681 aaaaaagctt atgaagatgt agatgtaata ttaatagctg taggtacacc tgagagaagt
    16741 gatggctcag ccaacctaga ttatataaag gcagttgcaa agcaaatagc tgaaactgtg
    16801 acaaaagata ctctagtagt aataaaatct acagttccaa ttggtacaaa tgaagagata
    16861 gaagattata taaatagaaa cctaaaaaat aatgtaaaag tagagcttgc atctaatcca
    16921 gagtttttat cacaaggaac agcagtaaaa gatactctat atgcttcaag aatcgttata
    16981 ggtgtagaaa gtgaatgggc tagagaaata ttagaagaaa tctataagcc ttttaatcaa
    17041 cctataatag ttacaaatag aaatagtgct gagatgataa aatacgcatc aaataacttc
    17101 ttagctctta aaatatcatt tatgaatgat attgcaaatc tttgtgaaat agcaggagct
    17161 aacatagaag atgtagcttt aggaatgagt tatgatgata gaataggaag taagttttta
    17221 aaggcgggaa tagggtatgg cggttcttgc tttccaaaag acacaaaagc tcttcactgg
    17281 ttatctgaac ataatggtta tgaattaaaa acagtaaaag ctgcaataga agttaatgaa
    17341 agtcaaaaac ttaaattagt tagaaaggca tctaagttag tagagagctt tcaagatatg
    17401 aaagttgctg ttttaggttt gacttttaaa ccagacactg atgatataag agaagcacca
    17461 tctataccaa atataaaata tttgcttgaa agaggggcaa gagttactgt atacgaccca
    17521 attggtgtaa ataatactaa gaaaatattt ggtgactcta tactttatgc tgattctgta
    17581 gatgatgcga tagaagatgc agagatatgt tttataatga cagaatggaa agatgtatta
    17641 aattatgata tacataagta taaggaaaag atgaaaaatc ctttagtcct tgatggaaga
    17701 aattgttata aaattaaaga tatggaagag ttaggaattg agtattattc gataggaagg
    17761 taaatattat gaagactgtt tgttatttag ctgatgggtc aaatcctcat actttaaaat
    17821 ggtgtagttt ttttaaaaat aaaggatatg acattcatgt aatctcttta aatggaggtc
    17881 acatggaagg aataaaaatt tataattttg gttctaatgt agaagaatta aaaaatgaaa
    17941 atatttctaa aaaaacggga tacttaggtt caataaagca gataaagaag ctagtaaatg
    18001 aaataaagcc agatatattg catgcacagt atgcaagtag ttatggattt ataggaagtc
    18061 tacttgggta tcatccatat attatttctg tttggggtac tgatatttat gatttcccta
    18121 ataatggttt tatacaaaaa caaattataa aacataactt tagaaaagca gactatatct
    18181 tttcaaatag tagagatatg gctaaagagg ctaataaata tacagctaaa catgtagatg
    18241 taaccttttt tggagtagat atggataggt ttaagcctat ggaagtagaa aaagaagacg
    18301 cttttgtaat aggaattata aagagtttag aaaaaaaata tggaatagaa tatttaatac
    18361 aagcatttaa gatgcttaaa gatgaatata aagataagaa aataattctt aaaataggtg
    18421 gctctggaag tcagatggac aacctaataa atttagctaa agaattgggt atagaaaatg
    18481 atgttcagtt tttaggaaga atatctccag aaaatgtaag caagactttt aattcttttg
    18541 atgtaactgt atttccttct ttaagagagg gatttggagt ggcagctata gaatcagaag
    18601 catgtgaagt acctgttata gttacaaatg taggaggaca tccagaatct gtatgggaaa
    18661 atgaaactgg cttaattgta gaaccaaaac aaccagaaga aataaaaaat gctataataa
    18721 aactaatgga aaatgatgaa cttagactta atatgggtaa gaaagggcgt caatttgtta
    18781 gagaaaatta tgaggttaat ttaaacttta atgatataga aaaaatttat gattctatat
    18841 ttgataaata taaaaaataa aatgaaaaaa tacagcaaaa gatagatgtt gttggctatg
    18901 cttaggtgcg tagcttttgc tgtttaaagg agggcttcta gttatgcgag aaagtataga
    18961 tttagagaaa gaaagctttt ttaataggct ttttaaaaaa tataaattta gacatttaac
    19021 actattaatt ctagatattt gtagcgtact agtagcgttt cgaatgtctt taagcttgac
    19081 aggtaattta aatgcattta ccactaatga tataataatt tcagtgtgta tatatatagt
    19141 acttcatata gtatcattta gattgtttaa gtgctacaac acattatgga gatatgcagg
    19201 agaagaagaa attatatcta tttttgtggc aactttagct tatttaattc cgatttatat
    19261 aataaataaa ttattagggt ttgattatcc tataatgttt tatgtattaa atacaatatt
    19321 tattataatg tttacaagtg gcgcaagaat agcttataga gctataagaa tagtcatgaa
    19381 caagacatat tctagaggaa aggtttcaaa tatccttata attggtgcag gagatgcagg
    19441 agaaatggtt atacaggaac taaaaagaaa ttcagaactt aaaaaagtag cagtggctat
    19501 aattgatgat gataaagata aaattggaag aataatccat aatgtgaaaa ttgtaggaac
    19561 aacttcggat ataaaagctg tggtagaaaa gtacaatgta gatgagataa tcttttcaat
    19621 agcaaacata gagaaacgta gaaaaaaaga gattattgat atatgtaaaa acactaactg
    19681 taaaataaag acaataccag gaatttacga aattatagat ggaaaagttg atataaaaca
    19741 gattagagaa gtagagatag aagacttgtt gggtagagag cctataaaaa caaatttaag
    19801 agagataagt aattacattg agggaaaagt aatcctaata actggaggag gaggctcaat
    19861 tggttcagag ctttgcagac agattgcagg atttaatcct aaagagctta ttatagttga
    19921 taattatgaa aataatgctt actcaataca acaagaatta cttagaaaat ataaaaataa
    19981 attagattta aagacagtaa tagcatcaat aagagaagaa aaaagaatgg atgaaatatt
    20041 taataaatat aaaccagaag tggtatttca tgcagcagca cataaacatg ttcctcttat
    20101 ggaatcgagc cctggcgaag ctataaagaa taatatattt ggaactttaa acatagctgg
    20161 cttgtcttca aaatatagag ctaagaaatt tgtccttata tctacagata aggctgtaaa
    20221 ccctactaac ataatgggag ctactaaaag agctgctgaa atgattatac agactatgaa
    20281 tgctgaaagc cagactgaat ttgttgctgt tagatttgga aatgtgcttg gtagtaatgg
    20341 tagtgtaatt ccacttttta agaagcaaat tgaagatggc ggaccagtga cagttactca
    20401 tccagatatc attagatatt ttatgacaat accagaagca gtaggcttag ttatacaagc
    20461 tggggctatg gctaaaggtg gagaaatatt tgtgcttgat atgggagaac ctgtaagaat
    20521 attggattta gcaaaaaact taattaaatt cagtggattt gagccagatg ttgatataaa
    20581 aattgaattt tctggtctta gaccaggaga aaagttgtat gaagaactac ttatgtcaga
    20641 agaaggtctg ttggatacag agcataaaaa gatttttatt ggtagaccaa tagatgttga
    20701 tagagaaaaa ataacaaaat acttaaaact tttaagagaa attactaaca atgaagaggt
    20761 tgaaaaaata gatggtatta tgagggaatt agtgccaact tatattaaac cagaagatgc
    20821 taatatcaaa gaaattgcaa caactagaga aaagtagaat atatttctaa tttgtaacca
    20881 gggggagcaa aaagaaacaa atatatacta aaaagaaaca aaagattaca aaaagattac
    20941 aaaaagatta caaaaaaggt tgcaatttat tttgatgtaa gatagaatgt taattaggaa
    21001 ttggaaacga aggaacaaaa tgttaaattt tcgaaaggat taggtatttg agattatgat
    21061 gaatattaaa aataaaaaac atatattgaa aaaatttata gctatggtgt taatagcagg
    21121 agttgtcaca gtagaagcag gagcaataac ggctagtgca gcagagccaa ctaattcacc
    21181 tatgagtgca acagtagacc agtgtgattt tttaaatgta agaagtggag ctagtgcaaa
    21241 tgatgcagta gtaggaaaga taaacacagg agacaaagtt gaagttttag aattacattc
    21301 aaatggatgg attaaaataa aaagtgttga caatgttaca ggatgggtaa atggagatta
    21361 tttaactatt caaggtggaa atgtagatgc aaaggtacaa aatgttttaa acttagcatt
    21421 taagcaacaa ggtaagccat atagatgggg agcaactgga ccaaatgctt ttgattgttc
    21481 tgggttcact tcttatgtct ataaaaatgg agcaggggtt aatttaccaa gagtatcaag
    21541 atcacaagca acagtaggaa agaaagtatc aagagcagaa ttaaaaccag gagatttagt
    21601 tttctttggt tcaggtggtt caataaatca tgttggattg tatgtaggag attctaaatt
    21661 catacattca ccacaaacag gagatgtagt aaaagttact agcatggctc caggaactaa
    21721 ttatgcaaga agacttataa ctgctacacg tgttttacaa taataataga tattgattta
    21781 cataggattg aatattaaca ttaaatatat aattacattt aatgttttac atagagattt
    21841 aagagtttag taatattaaa atagtgatga acgttataaa aacagcaaag aagataaatt
    21901 tctttgctgt tttttttata ataaattatt tcatattata atcaaatatc aaatataaat
    21961 tttaactata ttattcaata tttgacagat aatatgaatt ttagatatac ttaataagaa
    22021 gtatattgta gaaaaaataa atttaaaaaa ttaaggggga tgaaatgaaa aaaataagta
    22081 tattagtatt atcattgata atgacactta ctatgtgtag tgtaagttca tttgccgatt
    22141 catcaaatga taaggaaatg agagctgctt ggatatctac agtttataat ttagactggc
    22201 ctaagactaa gaataatgaa gccaagcaaa aaaaggaata cacagattta ttggacaaac
    22261 taaaaagtgt aggaataaat acagcagtgg ttcaagtaag accaaaatca gatgctttat
    22321 ataaatcaaa tataaaccca tggtctgaat atcttaccgg tacacaaggt aaagaccctg
    22381 gatatgaccc attaccattt ttaatagaag aagcacataa aagaggaatg gaatttcatg
    22441 catggtttaa tccatataga ataactatgg cagatgagag catagataaa ttgccagcaa
    22501 accatccagc aaagaaaaat ccaagctggg tagttaaaca tggaaataag tattactacg
    22561 acccaggttt accagaagtt agaaagtaca tagtggatag tatagcagaa gtagttcaaa
    22621 attacgatat agatggagtt cattttgatg attacttcta tccaggagta agtttcaatg
    22681 atactgcaac ataccaaaaa tatggaaaag gacaaaataa ggataattgg agaagagaaa
    22741 atgtaaatac tctacttaga gatgtaaaag cgtctataaa gagtataaag cctaatgttg
    22801 tatttggtgt aagcccagca ggtatatgga gaaataagag tagtgaccca acaggttcag
    22861 atacatcagg taatgaaagt tatgtaggta catatgcaga tacaagagca tggataaaac
    22921 aaggattgat tgactatgta gttccacaac tttactggcc aattggtctt aaagcagcag
    22981 attattctaa attagtggca tggtgggcta atgaagtaaa aggaacaaat gtagacttat
    23041 acataggaca aggtatatat aagcaaggtc aatctagtta tggtggacaa aatatagcaa
    23101 aagaaatagt acaacaagta acattaaata gaaagtatag tgaaataaaa ggtagtatgt
    23161 atttctcagc taaagatata gcaaatagta cttctataca aaaggattta aagagtttat
    23221 attctagtag tgaagaacca gttactccac catctaatgt taaagtggag aaattaagag
    23281 gcgacgaaag atatgacaca gcagtagcaa taagtaaaaa aggttgggct acaaattcag
    23341 atacagtagt attagtaaat gggtactcta tagttgatgg cataacatca acaccacttg
    23401 ctacatcaaa tgatgctcct atactattgg taaataagga taatatacct acatccacta
    23461 agaatgagtt aaaaagactt aatccaagta aagttatttt aattggagga aataactcta
    23521 ttggagataa agttgagagt gagataaaag atacattgtc aaatgtatca ataaatagag
    23581 ttggtggttc agatagatat agtacatctt taatgatagc aaaagaatta gtaaaaacta
    23641 atccagtaga aaaactatat ataacaagtg gtacaggtga agcagattct ctttctatag
    23701 cttcaaaagc aggagaagaa aagcaaccaa tagtactagt atcaaaagat aatgtaagtg
    23761 atgaagttta caattggata tcagatttaa aagtaaaaga tgcttatttt ataggtggta
    23821 atctaagtat atctgattca gtaataaata agctggataa ggtcattaca aatgatgtat
    23881 ctaaaaatag aatagctgga gaaaatagag aagaaacaaa tggaaaagta atacaaaaat
    23941 tctatccaaa tgctgaatat tcatcaatgt ttgtaagtaa aagtaatcaa ttggtagatg
    24001 cattaacatc aggtccttta gcagctaaat taaaatcacc agtagttatg cttggaaata
    24061 gtgtaacatc tgctcagaaa actgctttag agcctaagaa aactgctcta gtatacgaag
    24121 ctggagatgg tataaatcaa aacacattaa acactttctt aaacttagtt aagtaataaa
    24181 aaagttaatt taaaatacag agtattttat aaatgtagat taaaatgaaa attatcatca
    24241 taaattagaa aataaaaaag aactatatgc attttgtaat caagtgtaca cagcaacttg
    24301 aaaataatta tgcgatatag ttctttttta gtaattaatc ttatactttt taataaactt
    24361 tatgttgaat tttttattgt ttaaactcta tgtcattaaa tataaaatcg tgtaatatgt
    24421 ctaaagtatc tgggtcaaat ctaagaaccc atccagcttt tccatatata ccacctgtac
    24481 tatgaatttc atcatcaata ggaaattcaa attgcttaat atttaagtca cccattttta
    24541 aaacttgagc tcctaaagaa agtattgcat taggcttcat gttagtttta acatatggaa
    24601 gaactgcatc taataaacct ggatatttag taacaggtaa agtttccgtt ttcttgatta
    24661 tagcttgtat aatctgtctc tgtctgccat ctctagcaaa ggctgtatcg ttgtgtctga
    24721 tacgtgcaaa agatagtgcc tgatatccat ttagagtctg ctttccagag tttctaatgt
    24781 attgtatgct acctttatca tcacttttat tccatttata agtttcagga ataaatttat
    24841 ttagttctcg tatttctcct ttttcaacag taacctcaac gccaccaata gcatcaatta
    24901 tatacataaa tgattcgaaa ttaacacaag cataattttg aatatcaata ttaaaattct
    24961 cttcaattgt ttgtattaat aaatcagctt gtccataagc ataagcatga gtaagttttt
    25021 gtttaccatg accagggatg tctacatagc tatcacgagc taatgatgta agtttgatat
    25081 cattgtgttt gttatcaata gtaagtatca tcatagcatc tgaccttgaa gaatcttcat
    25141 ttggtctagc atctgttccc ataagtagta tgttaattat cccatcttca tttttatggt
    25201 cattgttatc caataaatca ctacttatgc tcgagtcatg aatagcagat aatttataat
    25261 aaaaatatcc atagacactt atagggaata ttactaccaa gaaagccagt agtataacaa
    25321 atttctttaa ttttgacaaa agtacccctt ctttcttctt tttcctgtat attttttaat
    25381 agcatacact taattatatt aaagtattga gatatattca atacttgttt cagttacaaa
    25441 aaaaagacaa tgatataatt tataagaaaa tttatcgacc tatatgtata gttaaaagta
    25501 tatataatat attttttaaa agtaatttat attttaaaat aaaaacacac cccataaata
    25561 caaagcaatt tgcatttaag aggtatgtat ttatcatcaa attattggtt tgttttggaa
    25621 tttctaatct tcaaccataa tatctttaaa tatgaaatca tgcattgcat ctagttcata
    25681 ttcctcaaaa ggtattacat agcctgcatt acctattttt ctaccatttt cagtagaaag
    25741 aggaaactcc atagatttca aatttaaatt tcctatactt aaaagttctt ttcctaaaga
    25801 tattatttca ttaggattca tattagtttt tacatacgga agtattgtgt taactagatt
    25861 aggatattta gtgactggta aatccttaac accatttatt aaactctgta taacacttct
    25921 ttgtcttcta tctctttcat ctgtactatc attttttctt atacgagaaa atgcaagtgt
    25981 ctgatatcca tttaattttt gctcaccaga atttcttata tatttaagag aacctttatt
    26041 gtcattttta ttccatttat aagtttcagg aataaattta tttaattctc ttatttcact
    26101 cttatcaaca gtaacagtaa ctccacctaa agcatcaact atatccataa aagagaaaaa
    26161 gtcaactacg gcgtagtttt gtatatcaag ttggaagttt tcttctatag tttctattaa
    26221 taagttagct tgtccatata cgtaggcatg tgttaatttt tgttctccac gtcctggtat
    26281 atcaacataa gtatctctat ttaatgaagt tagcttaaga cttttatttt tattatcaat
    26341 agtaagtatc atcatggcat cagaacgaga aacttcttct cctggtcttg catctgtacc
    26401 taacaataat atgtttgtga tattttcttc gtttttatag tcattactac taagcgcctc
    26461 tgtatttata ctatcatctt gtatagtttt taatttagta aaaacatatc catatatact
    26521 aacaggaaat gctataacta aaagggctag aaatataacg aactttttga gtcctgacaa
    26581 taatatcccc tactttctaa attttttaat tacacatagt taattatatt aaaaatttgt
    26641 tacataagca ataaaaaaca caataaatta gcataagttt gtcataatat aattattttt
    26701 atagtatttt atagaagaaa gtgtgtagtt attattgatt ttattggaat ataattgtat
    26761 gaagacttaa atttttataa actgatgtgt atattgaaaa aaatattttc ctgaatgtag
    26821 ttatagcatc ttatgaaagg tgtaaattgt aatttaaaat ttttagtgta taataagaat
    26881 tgaaaattca aaccgataaa aattaataaa aaataattat aaattaataa tttgggggag
    26941 gaaatgtatg ataaaattaa tagcaacaga tttagatgga actttgttag atgaaaagag
    27001 cgaaataaat ccagagtttt ataaagtatt taaaaaatta agagagagag ggataatgtt
    27061 ttctgctgct agtggcaggc agtatcaaaa tcttataaaa aaatttgaag atataaaaga
    27121 tgatatgatg tttatatcag aaaatggaac actagtagta tataaaggaa aagaaatatt
    27181 atcaaatcct ttaaataaag aattagtaaa tgaaatcata gagacaacaa gaagtataaa
    27241 agggaaaaaa attgtaatga gtgggaaaaa atacgcttat atagaaagca aagatgaagc
    27301 gtttatacaa gaggttagta cttattatgc aaagtttaaa attgtagacg atttgacgaa
    27361 agtagaaggc gatatattga agatagctgt atttgacttc aaaggtgcag aacataataa
    27421 taatatctac tttgaaaagt tttctgatag agctcaagta tgtatttctg gagtggagtg
    27481 gcttgattta actgcaaaag gtgcaaataa agggagtgcc ataaaaaaag tacaaaaaat
    27541 gttagatata aaatatgaag aaactatggt atttggtgac cagttaaatg atgtggaaat
    27601 gatgaaaagt gcctatcata gttatgccat ggaaaatgca aatgaacatt tgaaacaaat
    27661 tgctagattt agagcaaaaa gaaatactga aaatggcgtt gtagataaga taaaagaggt
    27721 cataaaaatt ggatagtcat atgtatagat gaaaaagatt attttgaaga aatcaaaaaa
    27781 atatcaatgt aattttgata aataagttta tttatgcgaa aattacattg atatttaata
    27841 aacatcaata atagatattt atttttttat gatattcagt attttgtttt ctatttgagt
    27901 tgcattttca gcagaaatta aataaacata tctacctgct ttctcaatta tagtattatt
    27961 tgctatttca gaattgattt tttctccatg tccttctcca aaaggtgctt gtatcatatc
    28021 ttttttataa ttttctagag ttttatatat tttatctacc tcgtcagttt ttataacgat
    28081 aatatcttct agagataatt tttcagaaga attaattaca aaaccttgaa ttatgtcttc
    28141 ctctacatca ttaaaatagt taaagtcctt tgcatcaaag atttttgatt cttttataag
    28201 taaatcagaa gattttaatg ctttttttat ttttgatgta gaaacgtttt tgtaatcacc
    28261 acttgaagaa caactaacca ttcctaatga taagattatc gaaaaaaaca atattaaaat
    28321 ttttttcatg ataaacctcc aagtttttta aactctgtct taaattagtt tactacataa
    28381 ttatataaaa ataaatgtat ttaatgttac agttttgatt aataatgtaa tattttgata
    28441 ataataccta ttgagaggtg attagaaatg agaataccaa aacatgtagg aattataccg
    28501 gatggaaata gaaggtgggc aagtcaaaat ggtatgacaa aagataaagg atatgaaagt
    28561 ggaatagacc ctggattaga gtcttataga ttatgtagag atttaggctg tgaagaggtg
    28621 actttttatg gatttacaac tgataataca agacgtccat cagtgcagac aaaagctttt
    28681 acaaaagcat gtattgatgc tgcaaatata ttaatagatg aaggtgctgc tttattagtt
    28741 gtaggaaata caaaatctcc aatgtttcca gaagaattat taccatatac taatagaatt
    28801 aaagccaaag aaggtcaaat aaaagcaaat cttctggtaa attatgggtg gtattgggac
    28861 ttaaataata tatcaggagt agaaaatgta aataaaaata atatacagga ttacatacag
    28921 tcatttgatg tatccagact agatttaata atcagatggg gtggaagaag gagattaagt
    28981 ggtttccttc ctgtacaatc aatttatgca gatttttatg ttatagataa ttattggcca
    29041 gattttaaga gtgacgatat aaaggacgca tttagttggt atgatacaca agatgtcact
    29101 ttaggtggat aattaaaaat attctgattt aaggttgtag agtgttttaa attattgaaa
    29161 aaaaaacttc ttgatggtaa aataacacta ataatatgta aaagtgtaaa aatttatacc
    29221 aagaagggtg aagaaatgtc aaaaggcaat aataacaata attcaagaaa caaatctaaa
    29281 aagaccagtc atctgaatag aaaaaagcga aaattaaata agaaaaagtt agcagtgttg
    29341 atttgcttta cagttttatt tttatttatt gcatttaagg ctactcaagg tgtagtagct
    29401 ttggtaaaaa gtatggataa aagtaataaa actagtcaac aacaaaaagt taactcagaa
    29461 caatttgacc ttggtaatga agaggaaaat aaaaagaaaa aatatactgt atttatagac
    29521 ccaggtcatg gaggcaatga taaaggaact gaaagcaaga ccagtaatag atatgaaaaa
    29581 gacctaaatc tacaaattgc aaaaaaatta gcaaataaat tatctaaaca aaaggatatt
    29641 caagtagtag tatccagaac tgatgataca tatatatcat taaaggatag agctatctta
    29701 gcaaataata gttctgctga tgtgcttgtt tctattcatt taaatgcaga gaaaaatggt
    29761 aatacagcaa caggtataga aacttggtat aggaataaag ctacagatgg ttcaaaagaa
    29821 cttgcacaga cagtacaaag tactatagta tcctatgtta aagttagaga taggggaata
    29881 gttgaaaata actttgaagt acttagagaa tcaaatatgc ctgctatatt aatagagtgt
    29941 ggttttttaa caacaccttc agaggaacaa aaaataataa atgaaaaata tcaagaccaa
    30001 cttgcggaag gaattgttca aggtgtactt tcatacctag attctaaagg gaataagtaa
    30061 gttaacttta ctttaatgtg aggataaatt taaaagttta actgtgtaaa ttgtattaac
    30121 tttatttcgt aatgttggaa acattgtttg aatttagaaa ctatgaaatt agaaattatt
    30181 ttatctatat ctaatttcat agtttttttt gcaaaaaatt tatagttata aaatttaaaa
    30241 aaataaattt agtataatgt aatgtagtta tttatatgtg attttaactt gcattgattt
    30301 gctatcaatt gtgctttata attagtatat ataaagttta actgtaagat aatttaaaaa
    30361 ataggggaat ttatatgaat aaaaatagca tgacaaaaat ttacagttta aataaaataa
    30421 tttttattat aattggaatt ttaggaatag ttttacttac aagacatatt gaggtagata
    30481 ttaaagtttt aatttt
//
LOCUS       DAGQWR010000038        30434 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.038, whole genome shotgun sequence.
ACCESSION   DAGQWR010000038 DAGQWR010000000
VERSION     DAGQWR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30434
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.038"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            268..960
                     /locus_tag="MIW95_001457"
     CDS             268..960
                     /locus_tag="MIW95_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3150788.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            1156..3216
                     /locus_tag="MIW95_001458"
     CDS             1156..3216
                     /locus_tag="MIW95_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBY3150789.1"
                     /translation="MEKYRALKLVSLIIISISFPLTILLATIGLAEPKYNIQKTSTKS
                     GIFNISNDFYDSNIFDEGTVESLLSNFKISVDSQLTSEEKSKLQTKSKSIDLSSNDSE
                     YDKIMINKQASEEYLSYLKNIKVYVINTYTGVFYTNTSYGNLDDFKQKTKSYCNIEII
                     NNVGKSSYIKTINGEKFELNNLSMELNGVDEAFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVACLSLAIVCLLSIILYSRIKGEVLKRNNVFLLIYSKIPMEFNVILVF
                     ISIAILTMYALAVSYHIDVIVLVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYIKVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3422..3745)
                     /locus_tag="MIW95_001459"
     CDS             complement(3422..3745)
                     /locus_tag="MIW95_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBY3150790.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(3738..4439)
                     /locus_tag="MIW95_001460"
     CDS             complement(3738..4439)
                     /locus_tag="MIW95_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBY3150791.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(4717..6102)
                     /locus_tag="MIW95_001461"
     CDS             complement(4717..6102)
                     /locus_tag="MIW95_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY3150792.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(6210..7448)
                     /locus_tag="MIW95_001462"
     CDS             complement(6210..7448)
                     /locus_tag="MIW95_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBY3150793.1"
                     /translation="MELFIEVIKFNIYISLLILFFILFTKKISKYTFKFNYFICLLIV
                     VRMMFISKINLNILNINNTTINIYKHNLDNYIPYDQGITLDIFMYIWILGIIAISFYY
                     LRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTR
                     CKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIHWFNPILYLLKKYFH
                     ELCELSCDEIVIDKCTTSEIKEYAYIILNTIKYQKTLKYSSFVSQFLNKKNIILKKRL
                     NSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIAKINYNKNGETYGT
                     HIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFK
                     KTIPLYDKEGIKIIGRFEIN"
     gene            complement(7461..7829)
                     /locus_tag="MIW95_001463"
     CDS             complement(7461..7829)
                     /locus_tag="MIW95_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY3150794.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
     gene            complement(8357..8779)
                     /locus_tag="MIW95_001464"
     CDS             complement(8357..8779)
                     /locus_tag="MIW95_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBY3150795.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            9257..12160
                     /locus_tag="MIW95_001465"
     CDS             9257..12160
                     /locus_tag="MIW95_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3150796.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILVL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     ISIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            12193..12639
                     /locus_tag="MIW95_001466"
     CDS             12193..12639
                     /locus_tag="MIW95_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY3150797.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            12762..13064
                     /locus_tag="MIW95_001467"
     CDS             12762..13064
                     /locus_tag="MIW95_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3150798.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            13245..14651
                     /locus_tag="MIW95_001468"
     CDS             13245..14651
                     /locus_tag="MIW95_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBY3150799.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFSDPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVISMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            14995..16053
                     /locus_tag="MIW95_001469"
     CDS             14995..16053
                     /locus_tag="MIW95_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBY3150800.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYASPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            16128..16814
                     /gene="araD"
                     /locus_tag="MIW95_001470"
     CDS             16128..16814
                     /gene="araD"
                     /locus_tag="MIW95_001470"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBY3150801.1"
                     /translation="MLEILKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNVVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            16832..17479
                     /locus_tag="MIW95_001471"
     CDS             16832..17479
                     /locus_tag="MIW95_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBY3150802.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELNVEHICVLDLNGNVIEVEKGIKPSSEVLMHIEIYKIRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFESWKYPEEIKF"
     gene            complement(17652..19019)
                     /locus_tag="MIW95_001472"
     CDS             complement(17652..19019)
                     /locus_tag="MIW95_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBY3150803.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLRSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(19209..19297)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            19725..21845
                     /locus_tag="MIW95_001473"
     CDS             19725..21845
                     /locus_tag="MIW95_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBY3150804.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESILKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKEIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            21949..23202
                     /locus_tag="MIW95_001474"
     CDS             21949..23202
                     /locus_tag="MIW95_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBY3150805.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFEDIETYSKDSQKKREKEL
                     DKFINTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEIYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTQV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGFMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(23356..24417)
                     /gene="ytvI"
                     /locus_tag="MIW95_001475"
     CDS             complement(23356..24417)
                     /gene="ytvI"
                     /locus_tag="MIW95_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBY3150806.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLSLILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFLELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFIGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            24691..25431
                     /locus_tag="MIW95_001476"
     CDS             24691..25431
                     /locus_tag="MIW95_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBY3150807.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            25443..27221
                     /locus_tag="MIW95_001477"
     CDS             25443..27221
                     /locus_tag="MIW95_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY3150808.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            27388..28305
                     /gene="pfkB"
                     /locus_tag="MIW95_001478"
     CDS             27388..28305
                     /gene="pfkB"
                     /locus_tag="MIW95_001478"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBY3150809.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYAKRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            28342..30261
                     /locus_tag="MIW95_001479"
     CDS             28342..30261
                     /locus_tag="MIW95_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY3150810.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT        12020 a         3340 c         4939 g        10135 t
ORIGIN      
        1 atcaaaatac ttaaataaaa gtaatctgaa aataacataa ttcaaatcaa tttattcaaa
       61 taaatctcaa tttatcttaa atcttaagaa atatttaaga attaggataa tattttctta
      121 agatttatat tttattatat agttataagg gaaagttaca aaaatgtaag ccaaagtaat
      181 ataacaattt agtcattgtt gagaaagtaa gtgaaagata ttgtataata tagataagta
      241 ttattataca atagaggaag tgaaaatatg tacaatatat tagtagtaga cgatgataaa
      301 gaaattgttg attcaataga aatatatctt aaatcagaag gatttaaaat atataaagca
      361 tatgatggaa tgaaagcttt ggaaataata acagagaatg acattcatct aatactaatg
      421 gatataatga tgcctaaatt agatggaatc aaagcaactg taaaaatacg agaagaaaga
      481 aatattccaa taattttaat ttcagcaaaa agtgaagata cagataaaat aataggatta
      541 aatattggag ctgatgatta tataacaaag ccatttaatc tattagagct tatagctagg
      601 gttaaatcaa acttaagacg atatgtaact ttaggtaatt atgaagatac atgtaaagaa
      661 attttaaaaa gtggagcttt ggagttaaat acactaacaa aagaagttaa aattgatggt
      721 gaaaacataa aaatgacccc aattgagtat aaaataattg aacttctttt agaaaataaa
      781 ggacgagttt tctcaataga tgagatatac gaaaaagtat ggaatgagga aagttttaat
      841 tcagaaaata cagtagcagt acatatcaga cgaataagag aaaagattga aataaatcca
      901 aaagaaccaa gatttttaaa ggtggtgtgg ggagttggtt ataagattga aaaattttag
      961 tggaaaaatt ataaaagaaa agagtttaat atttaattgc attttgattt tagtcatttt
     1021 tacatttaca caaatgttag gaattttaat atgttttaat tttttctctg agagtttaat
     1081 ttactctatg gggattatgc ttttcttaaa ctttatttta tataaaatac tgacaatgta
     1141 gatgaggtgg gcaacttgga aaaatataga gcattaaaat tagtttcatt aatcataata
     1201 tctatctcat ttccattaac tatattgttg gctacaatag gacttgctga gcctaaatac
     1261 aatatacaaa aaacttctac aaagagtggg atttttaata tatctaatga tttttatgat
     1321 agtaatatat ttgatgaagg aacagtggaa agcttattat ctaattttaa gatatctgtg
     1381 gatagtcagt taactagtga agaaaaaagt aaactccaaa caaaaagtaa aagtatagat
     1441 ttaagtagta atgattctga gtatgataaa atcatgatta ataaacaagc ttctgaagaa
     1501 tatttaagtt atctaaaaaa tataaaagtg tatgttatta atacatatac aggagtattt
     1561 tatacaaata catcatatgg taatttagat gattttaaac aaaaaactaa gagctattgt
     1621 aatatagaga taataaataa tgtaggaaag agctcgtata ttaaaacaat aaatggagaa
     1681 aaatttgaac tgaataatct gagtatggaa ctaaatggag tagatgaagc ctttgaggca
     1741 tatgtctctt ttccagaaga gccaactata gaagatggaa tagtatacac taattttcaa
     1801 atatttaaac aagctacaga aaaagtaaga ttattttttg tagcttgttt aagtttagca
     1861 attgtgtgtt tattatctat cattttatat agcagaataa aaggtgaagt tttaaaaaga
     1921 aataatgtat ttttactaat atacagtaag atacctatgg aatttaatgt aatactagta
     1981 tttatttcaa ttgcaatttt aactatgtat gctcttgctg tatcttatca tattgatgta
     2041 attgtattag tttctttatc tttactgata gtatcaaata atatattttt tataaatgtg
     2101 caattgagtt atttagaaaa taaaatgtgt atatttaaga caagtattat agtaagagtg
     2161 attttatggt taataaaaac tgtaagggag gtcagtaacg ctataaagaa agtatcttta
     2221 gcaaggaaag ttattgttat agtgataata tctttagctt taatcatggt aattagcact
     2281 cgaagtatag gtatgggatt actgttttct atatgtattt ttttaggatt tacaatatat
     2341 ataactaaaa gactttcata tcttagttac gtaatagaag gtacagaaag aataaaaaag
     2401 ggagaattag attataagat aaaaattact gggaatgata attttacgtc tttagctgaa
     2461 aatataaata atattggaga aggtcttgat agagctatag aagagcaagt aaaaagtgaa
     2521 agaatgaagt ctgatcttat aacaaatgta tctcatgatt tgaaaactcc acttacttct
     2581 ataataaatt atgtagattt aattaaaaaa gaagaatcaa tacaaccaga gtatataaat
     2641 gattatataa aggtacttga gtcaaagtca aaaagactaa aattattgat agaagattta
     2701 tttgaagcga gtagagtaag tagtggcaat atagaattac aaattagcaa gattgattta
     2761 gtgcaactat taagacaaag cataggtgaa cttgaagaaa aattatctaa aaataattta
     2821 tatttaaaat taaatgttcc aaatgataag gtgtatatat gggcagatgg aagaagaatg
     2881 tatagagtat ttgaaaatct tttatctaat atagcaaaat attccttaga agaaactaga
     2941 gtctatattg atgtatatga ctatggtgaa aatgtaaagg taactatgaa aaatatatct
     3001 tcttatgaat taaattttga cccaagtgag attatggaaa gatttaaaag agctgatgaa
     3061 tcaaggaata ctgaaggaag tggattaggg cttgctattg caagagattt agtagcttta
     3121 caaggtggaa aattttatat agaaatagat ggagatttat ttaaagcaaa tttagagttt
     3181 caaaaatatg aagaagaaat aacaaatatg gaatgagata gtatttaagt atatatattt
     3241 tttttactgt cgaaagcttt tattataata tattagaata tttacagtag agaatgaaga
     3301 gtaaaaacag agtaacttta agtcactctg ttttttattt aaaatcaata atataacaac
     3361 ttaaattaaa attgtacaca tagttttaaa ataacttata caataaaaca ttatccaaat
     3421 atcacaagat aattccaaat aaatatatag atatcactcc tgtaataata gaaataccaa
     3481 tagatttaaa cttatatgca acaagaagta caatcataaa agatataatt ttagggttag
     3541 ctatagataa atatagacta tctccattaa aaaatacatc tttagcagtt aaggaagtaa
     3601 gtattgctac aggtataaat ttcatgattt ttttcaaaaa atcaggcatc tctttttttg
     3661 aaaaaattaa cataggaagc actcttggta gatatgtaac aatgtacatt ccaattataa
     3721 taaaaaatat ataattatta ttcataagat tcctcctttt tacagtaaga ttcttctata
     3781 aagaaaccaa ttaaagatgc taaaattgat gcaactatta ttactaaatt gcttttgaat
     3841 aatgcatata aaataacaga aataatgatt gatgatataa atactacaat ataaatctta
     3901 ttttttattt gagaaactat taaagctata aacatagaag tcaatatgaa gccagaaata
     3961 gaactgtcta tagatatact ttctcctaaa aatgctccaa taaaattagc taaaactgat
     4021 gaaattaagc agaagaaatt aagataaata gcatttctat cagaccaatt tccagcagta
     4081 aatttttcat aattaactgc aaaagtttca tctgttatag tatgacaaaa tggcaatata
     4141 aattttggac ttttattttt tatgtacatg ttcaaattag aactcattag aaaatgtctt
     4201 aagttaacta taaatgtagt caaaacaata gatgttacac ttgcaccagt agaaatcatt
     4261 gaagctgcaa tgaattgaga acttcctcca aatactaaag ttaccattag tgctatttga
     4321 aaaggtgaaa gaccagattt ttgtaatagt attcctccta ctatacctat aggtatataa
     4381 cctaagaata taggaattga agcaataaag ctttctttta attgttgagt tctttccaca
     4441 ataaatcact ccttttgtat taaatgtatt aaagtattag taaaacagaa aaatagatta
     4501 attgtataat tgcaattata attaaaaata aatttggatt aatatataaa atactataca
     4561 acaaataaat aatcttaaac ggttattaaa ttttatctat ttgttattat gatgtaataa
     4621 tacatattaa tctctataag ttaattacta tctggtgtaa ttttatttta acaacaaaaa
     4681 aaaatacaca caattaaaat attgtgtgca atacaattat tttttctctt ctaatagtct
     4741 ttttaatacg ctcagaccag ctttaagttc ttctgtgctt tctgtagcac atacagctat
     4801 tcttattgca gaaattggtt tttcttttcc aacagcaaaa cgttcagaag catagacctg
     4861 tacaccttct ttaagtgctt gtatctcaaa tttttctgcc attattccat caggtagaat
     4921 caaccatcta aatatacatt cttcatttcc caaaagatta tatccagata aatattgatt
     4981 aataatcttg tttcttcttc ttgcagattt acggcgtgca ttaattaatt tatcggcttc
     5041 acctgatgta atcatgcgat aagcaatttc tgttaaaaaa taagattgag atagattaat
     5101 gttgtataat gcgcttgata atgcctctcg atactgttta ggtgtagaga tatatgctag
     5161 ccgtaaacta ggtgcaataa tttttgataa actcgtaata taaattgttt gattgggcag
     5221 ataggaagcc actggattta aatgtgtttc attaagtaaa ctatgaatag catcctcaat
     5281 aacaattaaa ttgtatttag atgcaatatt tgcaatcatt tttcgtccat tttgtgacat
     5341 tatatgtgta gttggatttt gataatctgg tattatgtat agacctttaa tattttcatt
     5401 tttacaagca tataatagtc cttcttcact aatctcatta tcttcttgtt taattggaat
     5461 tagttgaatc ccaagcattt tagctgctgt ctttattcca ggataagtta atgggtctac
     5521 acctattcta tctccatgtt gaaacaaacc agctaaaata gcaacaattg cattttgtcc
     5581 accacttgca ggaagtaagc tatctgatgt agtttcaagc ccagctttat aaattaattt
     5641 ggcaatagct tctttttgcc atttagcacc atctctttgt ccatattgga atatagtttt
     5701 aaaatctatt tccttaatta tatgtttaaa caaagtattc atttcttcta gtgtgaaatc
     5761 tggattcatt gtgcccattt caataaatgt aattttatta ttactggaca tcaagaatag
     5821 atttgaacgc gtatcataag atacaaaagt tccacttcct gtaactccac ttattagacc
     5881 ttttttttca caaattttaa aagctctaga gatagtactc acattaacat ctaagaaatc
     5941 tgccaattca cgttggggtg gtaatttagt acctggtaat aaaaatccat tgttgatatc
     6001 ttgttctagt tgttctgcaa gcgccttata taatatttgc ccttttgaag gtctctttgg
     6061 tttccagttc ataggataat tttcaaaaga attaattggc ataagttttc acctcacatt
     6121 attaatttga ttttttcttt atctaaaaca ataaattcta aagatacaaa taaaaactga
     6181 taggaaataa ttctatcagt ttttgtcttt taattgattt caaatcttcc tataattttt
     6241 ataccttctt tatcataaag agggattgtt tttttaaata tattaaaatt tctttttctt
     6301 gtatataaaa cagcttcttc tggtgtcttt ggttgattat cacaatcgta caaatctctt
     6361 ttttttacgt agccgacttt tttacctcta cctattgttt gtataagttc tggttcaatg
     6421 gaatttccat ttgagtctat tatatgtgta ccataggttt ctccattttt attataatta
     6481 atcttagcaa tagtatattc tacatcttca ttttgtttaa aattggagtt gatagaaaat
     6541 attgacccta ttattataaa ggttaataaa aacttaagac ctaaattaat tttcttttta
     6601 tcttgattaa acatagaatt taacctcttt tttaaaataa tatttttttt atttagaaac
     6661 tgagatacga atgatgaata cttcaaagtt ttttgatact ttattgtatt taatattata
     6721 tatgcatact ctttaatttc actcgtggta catttgtcaa tcacaatttc atcacaagat
     6781 aattcacata attcatgaaa atattttttt aataagtata aaattggatt gaaccaatgt
     6841 atgatacata ttatactaat tagaagctta aataagttat cttttctttt ataatgaatt
     6901 aattcatgtt taaagataaa tcttaactct ttattattgt actttttttc tggaattatt
     6961 attttacagc gtgttaaacc tataatggca ggggaataaa tacctttaac cttatatatc
     7021 tgaagttttt tatttatact tagttctttt gcttgttcat ctaagatact agtaatatct
     7081 atttctaaaa tttctttttt aaaattttct atgtaattat aaaatttaaa atttgttctt
     7141 aaatagtaaa aagagattgc tataattcct aatatccaga tgtacataaa tatatctaaa
     7201 gtaatgccct ggtcatatgg tatataatta tcgagattat gtttatagat attaatagta
     7261 gtattattga tatttaatat atttaaattt attttagata taaacatcat tctgactact
     7321 attaaaagac atataaaata attaaactta aaagtatatt tgcttatctt tttagtaaat
     7381 aaaatgaaaa aaagaattag tagacttatg taaatattaa atttaataac ttctataaat
     7441 aattccataa aatacgtctc ctattttctt ttttttagcc attgttcaag ttcttttatg
     7501 tcatcatctg atatttcatt tccacaacct aatgatgata taaagctatg aatagaattt
     7561 ttatgtataa gctctaaaaa atcctttgtt tcaaatttta gatattcctc atgtttaact
     7621 ttaatagtgt ataaagtatt tctaccatct ttttcaaatt ctaaaaaacc tttatcactt
     7681 aatctagaaa gtactttacc agtagtacct tttgtccatc catgagaaat ttccatataa
     7741 ttagctactt ctctggctgg tattttatct atgcctttat cccatatgaa attcataact
     7801 aacaactcgg attttggtat ttttttcaat ataatcactc acctttaact tgattatatt
     7861 agacaaatgt ctaatagtca atataatata tcatcatgta taaagaatct ttatattatt
     7921 ttatgcttga gaactaagta gtatagattt atagataaaa aagatattga gagttttatg
     7981 gaagagggga atatattttc tattagagat aagaatatga catatatttt ttattgattt
     8041 gtacaaaaat actatatagt gaataaatta atatcttttc taaattagaa gaggataatc
     8101 ttattagata aagattatcc tctttattga aagtttaagg gatattagag attattaatt
     8161 tttctctctt tttttaacta aaagcaataa tacataaaat tattattgct aatgatactc
     8221 ttaatgtggt atatagtcat agtgtcacat gtttatcaat agttttttca ttaattattg
     8281 taacgaatag taggactcat aaaatactta gaaatatgtt tttagataga ttttaaatat
     8341 ataatctaac ttatatctat aaattaacac tattattctc caattccatt tctagttttt
     8401 ttataaattt attaaattct tctggttgcc tttttccaaa caatactctt acaagagctg
     8461 aattgatttt tccaaatata ttcctcttgc tctcactttc aataaaaata agttttgtat
     8521 tttctaagtc aatatgtact aattcatatc tagtaatagt actttctctt ttattgcttg
     8581 tttttatctc atatactcta tttttttcga aattagttat ttcaacctta aatcttttat
     8641 tatttttttg ttgtacaaat gttcctactg cttttttctc attaaaatta ggaaaattta
     8701 gtcttgcaga ttttgtaaat atattaaata tgtcttttgc agaatatttt aataaaactt
     8761 cagttttaaa tgtattcata tcgttttccc ttctgtatat aaatttaata tttataagtt
     8821 aatttttaag ttaaaatagt ataatatgcc atattaaatc atgttaaaac tatttatata
     8881 gctaaaacct tacctatata agtatgtata ctatcatata aaaataaaat cttaaaatag
     8941 aaagggttaa aaacacagtt tattataatt tttattgttg agtagatacg tactattata
     9001 tatgattgag caagtttttc aatcataaat ttataaagta ttttgcatta aaatttaaac
     9061 aaagagttaa attatttaag attatgaata actaatacat ttagtaaatt actaaatata
     9121 caaaatgtaa atacttatca aaatgtataa atacttatga catctagtta cttacagggt
     9181 ttaagtagaa aaaaatatca aagtaagtat ggcatgatag ttgcttatat atttatcaag
     9241 aaaggagtta ttaagcatgg agttgagaga taaacttgta aacatcataa atacagaaaa
     9301 taaaaaagat ccattaacag atgttcaaat agcaaagttt ttaagtacaa ctagagagaa
     9361 cataactaat ttgagaaaag aacttaatat aagtaattca aggcaaagaa gatatcctta
     9421 tttaaaatca gcaatttcgg caatattaca aaagaataag aatatttcta tatcagaaat
     9481 tacaagggaa ttgatgacag aaggctttaa tatatcgaga agagttgtgg aggaactttt
     9541 acctaaagaa agtttagaaa tggatgtagt agaggaatct gaagaagaat cgaatgaccc
     9601 atttgaaact ctgataggta ataaagggag tcttagaaat gctgtggagc aggctaaatc
     9661 tgctatattg tatccaccaa aaggattacc aacgttgatt attggggaaa gtggagttgg
     9721 taagagttta ttttctagac atatgtatga gtttgcaaag caaaaaaaga tagtaaaaga
     9781 aagctccaat tttgttgtat ttaactgtgc tgattatagt gacaatccac aattattatt
     9841 atctttgtta tttggatata aaaaaggtgc atttacaggt gctgaatatg atacaccagg
     9901 tcttgtagaa gaagctgatg aaggagtttt atttttggat gaaatacata gattgcctcc
     9961 aaaaggtcaa gaaatactat tttctatctt agatagggga aagtttagga gattaggtga
    10021 aactaatgct gaaagaaaag taagtattat gttgattggg gcaactactg aaaatgtaga
    10081 gactaattta ttattaacat tcagaagaag gataccaatg ctaataactt tgccaccatt
    10141 acatgataga ttactaaaag agaaaataga ccttatatac aatatctttc aacaagagtg
    10201 taatagaata aatgcaaaaa tatttgtaga taaaaatgtg attgaaatac ttgtactaaa
    10261 aaagtttagt ggaaatatag gacaattaca aaatatgatt caggttcttt gtgcaagagc
    10321 ttttatgaag tttataaatt ccaaagatga agatagtgaa tcgatagtag tagtagatat
    10381 aaatgaagtt ttaaagctta aagattcttt taaagatgct gcctttcaag agattgaata
    10441 tacagagata agaaaatatt tgaaaaatgc tatatttata ccatttaatt tagaagaatc
    10501 atcagtaaaa ggaagtcttt taagtgatga atatatatta ccagaggaca tatacaaaac
    10561 aattgaaaag aaatattatg acctaaaatc cctagacatt gaagatatag atatagagaa
    10621 tattttatgg acttttattt taaatagatt tgctgactta aagtttaatc tcgatagtaa
    10681 tgaagaaatt ttttcaatga gtgatttaaa tagttttgta agtgatagtt tagttaaact
    10741 ggtaaaagaa cttatgaatg atatagttga aaaaaatatg aatttagaag taaataaaaa
    10801 tatgttcaaa tatctagcaa tacacttaga agaagctata aaaagaatta agctaaatca
    10861 aaagataata aatgtaaatt tagaaaaaat aaagattgac ttttcaaaag aatatgaaat
    10921 tagtaaaagt tttgcaataa aaattgagga atcaattagt attaagattc cagatgatga
    10981 aattggattt ataacactct acataaagtc tgctctaaaa aatgaagtga aaaaagataa
    11041 agtagggtta ataataattt ctcatggacg tatagctaca gagactgtaa atgtagttaa
    11101 agaactttta ggtgtaaagt ttcctgtggc tatagatatg cctttagatg aaaaaccaat
    11161 aaatatatac aataaggcag tagaactgtc aaaaataata gaccaaggga aaggtatatt
    11221 gtttttggtg gatattggtt cacttacaaa cataggtcaa atagtaaata agagaaccaa
    11281 tataagcact aaaactattg atagagtaga cctcctaatg gcactagaag caactagaaa
    11341 agtttctatg ggagaggaag aacttgatga aatatttttt tctttgctaa aagataggat
    11401 gggatataac tatgaattag aaaaacacat agataaatca aatgcaattg taacattatg
    11461 tcttactgga gaaggaactg ctaagtatat aagtaaaacg ttggaagaaa agtatgataa
    11521 cactaaatgt tatcaaatga gtgcattgga tgaaaattta ttttgtaaaa ttgaaaatct
    11581 aaaagagaca aacaatatat tagctattgt tgggacaata aaccctaaga taccaggtat
    11641 aaattttata ccctataata aagaactttt caaaaatttg gatatctatc tatcaaaatc
    11701 aaaacaacaa gatgacagac cagttaaatc gtatgaaaga atgcttgatg aggatttagt
    11761 gatttttgaa ccagatatat attttaaaaa ggatttatta gaatatgtat gttcaattct
    11821 tataaataaa gaatatgtag aaaaagatta cttagattct gttttacata gagaagaaat
    11881 gcttccaaca tattctaaag gagctatagg tgtgcctcat ggagattctt ctactgtaaa
    11941 tagtacaagg tttgtttttg ttaaactaaa gaatcctatt gattggggtg taggtaatgt
    12001 aaatttcata tttatgccag tttttcaggc taatgataaa gagattgtta aaaatgttct
    12061 tgagatacta aaagatgcag aatttatgaa caatgccaat aagtgttttg ataaagatgc
    12121 ttttagaagg attatatttg ataaatttaa acatctgtag ccatagcaaa aaaattgaga
    12181 ggagttagag taatgaatgt agatttaata tcattgggtt tagaatctaa tgattatgaa
    12241 agtgttattg aagaattagg aagtataatg tgtaaaaaag aatatgtaaa agaaacatac
    12301 ataaatgctg tcttggaaag agaaaggaca ttaccaacag gtctagatat tggagaaatg
    12361 tgtgttgcta ttccacatac agattcaaag catgtaaatg agtctaatgt agctgtaggg
    12421 atattgaaaa atcctgtaaa gtttaattca atgatagacc caaaagatag acttgatgta
    12481 gaacttgtat ttttattagc agtaaagaac ccagattcac aagtaaaact gttaaaagat
    12541 ttaatgtcag tttttcaaaa tataaagtta ttaaaaaata taaaaaacgc atcaacaaaa
    12601 gaagaagtag ctaaattatt ggattttata gaaatttaat gtaggagaat tttataaata
    12661 aaaaattaaa atttcaacaa tcaaagtaag tattattttt ttaataggtt taatcttata
    12721 taaaataaaa atttagagtg aaaaattagg aggaaattat tatgaaacaa tttaatgtat
    12781 tatcagtttg tggttcaggg acagttacat catctatggt agcaggtaaa ttaaaagaag
    12841 tactaggtaa taaggggatt tctattacta caacagaagc tagaccaaca gaagcactaa
    12901 atttagcaca atcaggaaga tttgacttta taacatttac gagtccgctt caaacaggcg
    12961 attatggaat accaactata aatgcatttg cttgcctaac tggtataggg gaagaggcat
    13021 tctttgaaga agttttgact gttattgaag gcatagataa gtaagttttg gagacgtgaa
    13081 tttatagtat ttaaatcaat gatttagata ctataataag ctaatattaa ctaaatattt
    13141 gtggaaaaaa cattttatat gttgtcataa attaaaaaat tataaaaata gtcacttaag
    13201 tattaaatta agcttataaa tatttataag taggagggaa aaagatgtta gaatttatga
    13261 aatctataat cgatacattt ggttctgcaa ttatagttcc aattataata ttcataatag
    13321 caaagatatt taaagtaact actaagaaat cgtttctttc tgctgtgtat gcaggagttg
    13381 ctttacaagg ttttacactg attttaaatt catttacacc aataataaca ccagttatca
    13441 ataggatggt tgagagtaca ggagtaaacc taccagtttt tgatgttggt tggcaagcaa
    13501 catcattggt tgcattttca acaagtgcag gtatgatata tttaggtctt ggtatattat
    13561 tacaaacaat tttatttttg ataaaatgga cagacgtgtt tcaaccatca gatttatgga
    13621 ataactattc ttatatggta tggggagcaa tggttatagg agtaacagga aattttcctt
    13681 taggtatagc atgtatggtt ttattaaact tatatagttt attaatatca gagttagttg
    13741 caaagagatg gtctagttac tatagatatc ctaattgtac aataatagct atgcataatg
    13801 ttgaggcttc tgtgtttgca gtattttcag accctatata caataaattg ggattaaata
    13861 aaataaaatt aaatccaaaa gaattagaga agaaattagg gtttttaggt gaaccaatta
    13921 ctttaggtct ttttttaggt atgtttatag gtatattagg taatatgacg agaataaata
    13981 ctatggaagc ttggggagaa ataatgaaag taggtatatc tacaagtgca gtgatggcga
    14041 ttttccctaa agtagcaagt atgtttgctc aagcatttgc acctataaca gaagcagcta
    14101 gaaagattat gcaaaaagct ggaaaccgtg agtggtacat agcagtaaat gatgctgttg
    14161 gatatggtga acctgcaact ttaatatcag gtcttatatt aattccaata atgcttgtaa
    14221 tatctatggt acttcctggt aataaggtat taccagttgt ggatttgttg gcaattccat
    14281 atatggttca aggtttagtt gccatacata atggtaatat accaaaagta ttagtttcag
    14341 gtataatttg gtttggatta ggattatatg tttgtacatc tacagcacca ttatttacag
    14401 atatggctac aaatataggt gtagctatac ctgcaggagc aatgttaata acaagcttta
    14461 atatactagg aaaacctttg atgggattag ttttctttgc atttctaagt gcaaatccaa
    14521 tttatatagg tctttcagtt gtaatttact ttgttctatg ggcattgttt agaaagaaca
    14581 aaacaagtat tcttgattat ttagaaaaac aagcacttaa aaatgtagaa gaagagcctg
    14641 tagcagttta agtttataaa aatgaaataa gaataattaa aggagtttat aattgatttt
    14701 atataagtat cagataatac ctgagaaata tttaaatgaa atatagactt ctttttattt
    14761 gataatattt gtaattataa tgcattgtat ttttatagtt gagttttctg tataacctgc
    14821 tatttaaaac atactctttt atatttatac agtaatgtgg tttaatttta taaaatgtaa
    14881 tctctactgg taaaagactt tgtatataag gcatttgcct gaataaacca tttatctttt
    14941 gtatggcata gatattgcta tatagaagta tatagcatga aaggagatat ttatatggga
    15001 gataaaatga gagcatcagt attgtataat gtaggtgatg tcaggtatga aatggtagat
    15061 ataccagaaa taacagatac gcaagtgctt gtaaatgtaa aatatgttgg gatatgtggt
    15121 tcagatttgc ctaggtctat ggtaagtggt ttgagtggaa atacaaaata tccattgatt
    15181 ttaggacatg aattttctgg tgaagttgtt aaaattggag aaaaagtaaa gcatattaat
    15241 gtaggagata gagtagctgt tgcaccatta gtaccttgtg gaaaatgtga ttattgtaat
    15301 gaaggtaatt ttggcttgtg tgatgattac aatataattg gaacaagagt aaatggagcg
    15361 tttgcagaat atgtaagagt tccagaagaa catattttaa aactaccaga tacattggat
    15421 tatgaaactg ctgccggaat tgaaccagca actatagctt atcatgggat ttcaaagtct
    15481 aatattagag taggagatag tgtagttgtg ttaggatgtg gtccaatagg tcagtttgtt
    15541 atacaatggg caaaggtgtt tggtgcatct aagattatag cagttgatat ttttgatgaa
    15601 aaattagaac tttctaaact tctaggagca aactatatac ttaactcaaa agaagtaaat
    15661 gtaatcaaag aaataaaaaa aataacaaat ggtggtgctg atgttgtaat agagactgct
    15721 ggtagccgat ttacacaaga acaatctcta tttgtagcaa aaaagagagg aaatatagtg
    15781 tttgtaggta tatcacacac agaattacca cttagtgcag acgctactga gtgtatatta
    15841 cgtggagaac ttaccttgaa aggttcttgg aattcatatg caagccctta tcctggaaga
    15901 gcatggactg caacattgga ttttatggga aaaggtgaca ttatttttaa acccatgatt
    15961 tcacataaaa taggtcttaa tgaagtgggt gactttttaa gtaaaatgag caagagagaa
    16021 attaatttca ataaaatcct agtagaaata taaaaattta ataaaaaatt ctaaaaacaa
    16081 ttaaatttta taactatgac attaaataaa atataggagg aagaaaagtg ctagaaattt
    16141 taaaagaaga agtattaaaa gcaaacttag aattacctaa gaaaaagcta attacttata
    16201 cttggggaaa tgtaagtggg atagatagag aaaaaggatt agtggtgata aaaccaagtg
    16261 gtgtagagta tagtaatatg actgtagaag atatggttgt agttgattta gagggaaatg
    16321 tagttgatgg caaactaaga ccctcctcag atactccgac tcatctagtt ttatataaag
    16381 agtttgaaga attgggggga atagtacata cacattcaag ttgggcaaca atttgggctc
    16441 aaataggaaa atctatacca tcttgtggta ctactcatgc agattatttt tatggaagca
    16501 taccatgtac cagaaaaatg actagagaag aaatttgcaa tgaatacgaa aaggaaactg
    16561 gaaatgtaat cattgaagaa tttatgggaa gaaatccaat tcattgtcca ggaataattg
    16621 taaatgacca cggacctttt acatggggaa aagatgcaaa tgaggctgta cacaatgcag
    16681 tagttttaga agaagtcgca aagatggctt attatacaga gttaatgagt cctgataata
    16741 ttatggataa ggttcttatg aataagcatt tttcaagaaa acatggaaaa aatgcatact
    16801 atggtcaaaa atgattatct ggaggtgaat tatgctagac tctttaaaag gtaaagttgt
    16861 ggaaatagca attaaagcag agcattatgg tttatgtaaa gaaaagactg gtaattttag
    16921 tattagagat aaggatactg gatatatact tataacccca tcaggtatag gaagagaaga
    16981 attaaacgtt gaacatattt gtgtattgga tttgaatgga aatgtaatag aagttgagaa
    17041 aggtattaag ccaagtagtg aggtattgat gcatattgaa atatataaaa taagggaaaa
    17101 tataaatgct atatcacata ctcattcttt atatgcaaca gcattttcag tagcaaagaa
    17161 accaattata ccaattgtat atgaatcagt taattatggc ggttatgtag atattgctcc
    17221 atacgaaaga ccaggaactt taaaattagc taagagcgtt tcaaaattac tgatagatac
    17281 agatgcatgc ttattagaaa gacatgggtt gacaactgta ggtaatgatt tagacgaagc
    17341 attattaaaa tcaagatatg ttgaagaggt agcagaaata tactataggt ctttagtatt
    17401 aaatcaattt aaagaacttg aagtagtaga tgttgatgag tttgaatctt ggaaataccc
    17461 agaagaaata aagttttaat ctaaataatt ttcattgaag tggttaatta aaatatatgt
    17521 attatttata tcaattacta tgttttatta tattaatcac cgtattttgt aaatatattt
    17581 attacatttt ttcaaaaaaa tatagaatcc gaaagccata taaaattgac ttgggattct
    17641 atacttttta tttatgcatt tttctttatc tgattttctg gagaatttat atgttcaaaa
    17701 tattttttag tttcagatac tattataggt gataatgcaa gtaaagctat aaggttaggt
    17761 attgccataa gcccatttac aatatcagca attatccaaa caacttctaa ctttaagaaa
    17821 gcacctaata acaccatagc cacaaataca gttctatacc cattcatgcc ttttacacca
    17881 aacagaaatt caaaacatct ttctccataa taactccatc ccaaaattgt agtaaatgca
    17941 aataatgtta aactaactgt aagtaatatt ggtccaaatg taggaattgc tccgttaaaa
    18001 gctgcttgtg tcataacagc tccatttagg tctgaacacc acactcctga aataacaaga
    18061 gagaatccag taagagtaca gattataata gtatctataa aagtacctgt catagatata
    18121 agaccttgtt ccgctggcca ttcagtttta gcagcagctg cagctattgg agcactacct
    18181 agaccagact catttgaaaa tacaccacga gcaataccat ttctaattgc cagtgacata
    18241 gttgctccta aaaaaccacc agtagcagca gtaggtgtaa atgcactttt aattattaac
    18301 ataaaagttt cagggatttt attccaaaaa gatattaaaa tgattccaca gattacaaca
    18361 tatataactg ccataaaagg tactatttta gtagctacat tagatatact tctaagtcct
    18421 ccaaatacaa ccaaagctac aaatactgct aatacaaccc ctgtaattct agggtctatc
    18481 ccgaaactat tatttattgc atctgttatt gaatttactt gagtaaaagt accgattcct
    18541 aaaagtgcaa ctagtatacc acttatagcg aaaaatattg caagaggtct ccatttttct
    18601 cccataccat ttactatgta atacattggc ccaccagaca cttgaccatt tttatcttta
    18661 gttctgtatt ttattgcaag aacaccttct gaatacttag tagccattcc aaagaatgct
    18721 gctatccaca tccaaaaaag tgcaccaggt ccaccagctt tgatagctgt agcaacacca
    18781 acaatattac cagtaccaac tgtagcagct agagcagtac ataaggctgc aaaacttgaa
    18841 acatcaccac tacctttgtt ttcagcagag aatattaact taagagctct tggcagttta
    18901 gttatttgaa caactccaag cttaacagta agtagtatac cagtaccaac taatagtacc
    18961 agtaggggtg gtccccaaat aaaagcatca actttgttta aaatatcaat tagattcata
    19021 aaaacactcc ctcatttttt ttgctttaca taatcaatag aatttttaat gaagtaattg
    19081 acaggaaata agtataattt attaataatt tctagtgtaa taaaaaaagc taaatctaaa
    19141 aaagattcag ctctggatgt acactaatta gcaaccaaaa taaaagtatt agtaaatact
    19201 gtttttggta gataatccct gtccttttgc ctgagagatt gagcataaaa gccttgcccc
    19261 ttcggcgccc ataatgggtc tctccagagt tccgtaaatt ttacagtcct acttttaaaa
    19321 gtacctgaga gtgttactcc ttcggtgtgc tatatagcaa ctcttctata aaatcttaac
    19381 cggatattga tttgtcatag cttattgtat taataatatt aatataagtt tttatgtttt
    19441 gtcaactaaa aaattaaaaa acaaagaaaa aatataaaaa tgtaagccta tttacgaatt
    19501 ataaacttag aaagatgagt aatatacgta aaaatgaatt ttgtaataca catacaaatg
    19561 aagtgtaaaa taagttttaa ataaaaaaat aataaaatct taaataatta attagatatg
    19621 attaaattat ggtaatatta tagttacgat attaagaaaa gttctattta gttagatttt
    19681 aagcgtaata tgagattatg tatagagaaa ggatgtattt ttagatggga aaaatattag
    19741 tgctagcaga aaaaccttct gttggaagag atatagcaaa agttttaggg tcaaaaaatg
    19801 aaaaaaatgg ttatatagag gggccaaagt atgtgataac ttgggcacta ggtcatttgg
    19861 ttacacttgc tgaccccgaa agttatggag aaagatataa atcatggagc ttagaagacc
    19921 tgccaatact tcccaagcac cttaagactg ttgtaataaa aaagagtgga aagcagttta
    19981 atacagttaa atcgcaaatg aatagaaatg atatagatga aatagttata gcaacagatg
    20041 caggtcgtga aggagaactt gtagcaaggt ggataataga aaaatcacag gtaaaaaaac
    20101 ctataaaacg tctttggatt tcttcaagta cagacaaagc aataaaagac ggatttgcaa
    20161 aattaagaag tggaaaagaa tatgaaaatt tgtattactc agcaatagca cgagcagaag
    20221 ctgattggat tattggaata aatgcaacca gagctttgac aacaaagtac aatgcacagc
    20281 tttcttgtgg aagagtacaa actcctactc ttgcaatgtt gcttaaaaga gaagaagaaa
    20341 taagaaattt caaacctaaa gagtattatg gattagaatt aattgctaca aagggaaatt
    20401 cagatataaa atttatttgg aatgataaaa acaataattc ttcaactttt agtaaagaaa
    20461 agatagaatc catattaaaa aaagtaaaag gtgtagacat aaaaatatct gaagtaaaca
    20521 agtcatataa gaaaaaatat tcacctgcac tctatgattt aacagaatta caaagagatg
    20581 caaataaaat gtttggattt tcagcaaaag aaactttatc tataatgcaa aaactgtatg
    20641 aacatcataa agtacttact tatcctagaa ctgattctag atatttaaca tcagatattg
    20701 ttggaacatt aaaagataga ataaaagctg taaatattag tgattattca aagatttgta
    20761 ataaattatt aaaaagcaat attatagcta ataaatcgtt tgttgacaat tcaaaggtaa
    20821 cagaccacca tgcaataata ccaacagaac agaaggtatt catgagtgat tttagtgaca
    20881 aagagcgaaa aatatataat cttgtagtaa aaagattttt agcagtacta tctcctcctt
    20941 ttgaatatga gcagacaact ataagtgcaa ctgtagaagg agaaatattt aaggctaagg
    21001 gaaacaaagt aataaattta ggttggaaag aaagttataa agatattgaa gatgaagatg
    21061 agtatgaaag tataccaaat gttttagaaa aagatgtact taaggtcaag ggattaaaga
    21121 taacttcagg aaaaacaaat ccaccaccat acttaaatga gggaacatta ttgactgcta
    21181 tggaaaagaa tggtctaggg acagttgcta caagagcaga tataatagaa aaattattca
    21241 attcattttt aatggaaaag aaaaataaag agatatatat aacatcaaaa ggaagacaat
    21301 tattagattt agttccagta gacttaaaga caccagaact tacagcttca tgggaaaaga
    21361 aattattgga tatttcaaag ggaaaactta gtaaaaatac attcataaat gacatgaaag
    21421 attattcaaa agatattgta ctagaagtaa aaaatagtga taataaattt aaacatgata
    21481 acttaactaa aaatagatgt ccagaatgtg gtaaatttat gttagaggta aatggtaaaa
    21541 aaggtaagat gttgatttgt gaagatagag agtgtaatac tagaaaaact atatcacaag
    21601 taaccaattc aagatgtcct aactgtcata aaaaattaga acttcgtgga gaaggtgagg
    21661 ggagaatctt cacttgtagt tgcggataca gagaaaagtt gtcttctttc aacaagagaa
    21721 agcaagaaga aaaacagaag gcatcaaaaa aagatgttaa tcaatattta aaaaatcaaa
    21781 ataaaaatca agagacattt aataatccat ttgcagaagc attagctaag ctaaaaaaca
    21841 aataatttta aagttattat tatacacaat tataacttta actgtatatt agttatatgt
    21901 actgatataa tatagtgtat agtttaggca tgaaagtagg gctgttaaat ggatttaagt
    21961 ggcatattta aatactattg taaagagtgt gaaaatacat ggaataattc gagtgttgaa
    22021 ttatttgagg atatagaaac gtatagtaaa gattcacaaa aaaagaggga aaaagaatta
    22081 gataaattta taaatacaat atcagttcat ttagagaggt atccaagtga tgctgtattg
    22141 agaaaaatgt gggtaaaaaa gggcgaggtt ttcttacaaa agacattgga aaaagaaaat
    22201 atttttaagt tagaaaaaat ggatgtagag gatagaaaaa aatttttaga tataacaaaa
    22261 cagtttatta gagatgctag aaaatttgat gatgatttac ctataggtga tattatgcaa
    22321 gctatgagga atgtatggat ttcaaatgca ttacaattat tatttggtaa agaaatatat
    22381 tattcaaaag ctaactttgc atatagtatg ttatatccat atactgataa ttatttagac
    22441 aatacaaata tagataaaaa tgataagatt ttatttaata actggttaga aaaaaggctc
    22501 ctgggagaac atattaaatc taaggattat catgaaagta aagtatctca aatgatagat
    22561 tatattgaaa gtgtataccc tagagaaaag tttacacaag tttatgaatc gttattatta
    22621 atatttaaaa gtcaagtaaa tagtttaaaa caacatggta aggaaaatca tttgtgtaaa
    22681 gaagatttat tatccatttc tattgaaaaa ggaggttcat ccgttttagt agatggatat
    22741 ttaataagtg gatttatgac aaaggaagaa atagagtttt gtataggata tggattttta
    22801 ttacaaatat ctgatgattt acaagatata aaagaagatt taaaatacaa ccataaaact
    22861 attattacag agatgtcaaa agagggtact ttagataaag ttgtaaataa actaataaat
    22921 tttactattg agttaataga tagttttaaa attaataata aaaataaatc tgtaataact
    22981 atgataaaga atgattgctt aatgttaatt ttattttctg tagtttataa tgctgaattt
    23041 ttttctgtag gatatataaa agaagtagag aaatttattc catatacaat agattattca
    23101 ttagagattg aagaaaaaat aaaagaaaaa tttaaaaata tagatgtttt aaataatgaa
    23161 aatgaatata aagaaatgat tgatattatt tgtgcagagt agaaaaaaag ggagtgtctt
    23221 agaatagact ttttagttta tgaggcactc tttttgtata aaatcaaatg tattttcatc
    23281 attttaacaa ttaaaaagag cttatccctg tttgagaaag ccctttttaa tttaaataaa
    23341 aatgtcaagg ttaaactatt taaatgcagt aataatccca ttatcatgta agttttttat
    23401 gattgtagct gcgataggga atccaaataa tccagctatt ccaaatagtt gagctccaat
    23461 aaacatacac attaatgtta ttaaaggata taggccaatt tggtgtccta ctacctttgg
    23521 ttctaatatt tgtctaacaa cggttataat tatgtatagt attaaaagtc ctattgctaa
    23581 attaatattt ccattaatga gtgagataat catccaaggt gtcaaaatac ttcctgtacc
    23641 caagactggc aaaatatcaa caagtgctat gatgacagat atagtaaatg cattttcaac
    23701 ctttaggata gtaagtccaa taagtagttc taagaatgtc actgatagta gtattgcata
    23761 ggcttttagt agttttaaaa gagcattaat actgctattt tttatattta ttatcataaa
    23821 ttgacttttt tctggaaatt gttttaagat aaagtttact attttttgat agtcaaaagt
    23881 aaagaaaaaa gaagcaatta aagtaataat aagttttaac ataaatgcag gtgttttact
    23941 tgcaatacta gcaatagcat tgagtgcatt tgttgaaata gaagctacta aagacatcat
    24001 agattgactt atattatccc aaccaagtat taatgatagg ttaacattag gtgtactagc
    24061 atctatcttt gaaaataaag tatttagcgt tggttgtata cttgatttat atatttcagg
    24121 aagtctaaaa aatacatcac ttacacttgc aaatatcttc acaccaaagc taactaataa
    24181 aaatatacac aacccataaa aaattaaaag tattactata gaaataagtg tacgatttaa
    24241 atttgtatta tttgtaataa gtcttataat aggtcttaag atggatgcaa tgataaaact
    24301 gattacaaaa ggcattaata atggcaataa gaattttaat acaatatata caatacttgt
    24361 tattaaaact acatatgtaa atttaattat aaaattcttt tgcttttgaa tatccataat
    24421 ataaaacctc caatgtattt gatattattc taaatatggt cttttttaca gaaaatatac
    24481 tactgtatat tgattataaa ctataaagct tatgtaaagt aaataaatta tgtaaaattt
    24541 aggttaattt tgtataaaga gatactttta tattttacaa ttaataacca aatactaaaa
    24601 aatattttaa ataaagaact taaaattgtg ataaaattaa tttatagata ttaaaaaaag
    24661 tataatatat ggtttttaag aggtgctaag atgaaaaatt ttagagattt aggtgggaac
    24721 aaaactgagg atggcagaac tgtaaagaag ggattatttt ataggtctgc aaaattatca
    24781 aatctaagtg aaaatgatat aaagatttta aaagagttaa atattaaata tatatttgat
    24841 tatagaagtg atgaagaagc tcgtaaacat ccatcaacaa taatatcaaa tataaaaaat
    24901 ataagaatac cagcaatgag agagctagaa gaatcaggag gaagttttgg ttctattgaa
    24961 gatatgattg atggattgtt tgaaaaagat ggtgcattta atatgttaaa taatagttac
    25021 tataatttgc ctataaataa tccgtcatat aaaaaattag tagaacttat aagagactat
    25081 tctaatttac caatattaaa tcattgtaca gctggtaagg atagaactgg tgtggggagt
    25141 gctataattc ttatgatttt aggggtatcg agagagaata taatgaaaga ttatctaaaa
    25201 agtaatgatt ttgcagataa agagatagaa agatttatag aatataagcc taaatttaag
    25261 gacattccaa aagaaaattt aaaatatata tttggtgtaa atgaagagta tatgaaaact
    25321 gcatttagaa gaattgatga agaatacatt agtgtagaag catatttata tggagaattt
    25381 aatttaaata aagaagaaat aagaaaatta agaaatcaat atctagagta aaaggtgagt
    25441 ttatgagttt aaatataaca gcaagacaga taaatataat aaaaatattg ttaaattcaa
    25501 aagatacaat tagtggtatt gccttaagtc aagagatagg atgttcttca aaaactatac
    25561 aaaatgaaat aaaggatatt aataaacaac ttagaaatgg taaaattttg tcaataagag
    25621 gagttggtta taaactagaa ggaagttttg atgaggtaaa tctaaatagc aatctctatg
    25681 atgatgtaga tagagtagaa tatataatca gaaaaatatt aaccttaagt aatgaagaaa
    25741 aaaatactat aaaacttgaa gatttagccg attctatgta tgtaagtgtt tcaacagtaa
    25801 aaaatgattt aaaagaagta aaaagaatat tggaaaagta ttcaatatca gttaaatcta
    25861 agcataaaca aggtatatgt gttttggaaa gtgaagataa gatattaaat tgtatagttg
    25921 acttaagcaa taagagagat aatcaattga gtttaaatga ttttttaaat gacgatataa
    25981 aggaaagaaa actattaata aaaaaattat tattagacat attaaatgaa aatggattag
    26041 tattaactga tatagaattt aagagtgtgt taaataatat attagttatg ttaagcagac
    26101 ataagtataa tgaaaaagaa tttatagaga attatataga aaattatagg agcaaaagaa
    26161 gctcaatttt agaaaatgaa caaaacaaag aaattataat aaattcaata aaaacatttt
    26221 gtaaaaattt aaaattagct acatcaatag atataagtaa tgataaggta tttgaagagt
    26281 ttttatataa gcatattagt agtttatgta aaaaaataaa gttaggtata aatcaatcac
    26341 ctataatggc tcaagatata aaaataaaat atccatttgc ttttgaacta gctaatatag
    26401 ctaaaaagac aatagaaaaa gatttgaaga tagaaattaa tgaagatgaa gttgcaaaca
    26461 tagctctaca tataggagga gcagttgaaa gagcatctta taataataaa gataaggttt
    26521 tcaaaactat aatagtttgt acttcaggca taggcacatc aatgttaata aaagctaaat
    26581 tagagaatat atttaaggaa aaattagaaa tactaaagat tattccttca tacttagttg
    26641 aatatataaa agttatagat gttgattttg taatatctac tgttcctatt gaagtaggag
    26701 atgtgccagt aataaatata tctccaattt taagtgagaa agaaattaga ttaattgaaa
    26761 aatatataga aacaggaaat atatacctag atttagatat aaaaagcctg tttgatagtg
    26821 aattattttt tactgatttg gtgtttaaaa ggaaagaaga agttatagat tttatggcaa
    26881 gtaaacttgt acaaaagggc tatatagatg aggatatgaa aaaatcttac tttgagagag
    26941 aaaaaatagc aacaacagaa atagggaata tggtaactat accacatgga gctattggaa
    27001 aaatttttga taataaaata gcaattggta tattaaagaa gcctatttct tgggaaatat
    27061 cagatgttcg tttaataata atgttagccc ttgataaaga taaaatatta gattatgaag
    27121 taatattttc aaaaatatat aaaagagtag attctatagc aaaagttata agtatatgtg
    27181 aaaatagtag ttttgaaaaa tttgtaaatt tatttaatta gatgggtagt attgtatata
    27241 cacctgtaaa tttggataat ataaaactaa ataagataaa aacgataaat ttctctttta
    27301 attaagagaa attttttgac tttcaaggat attaatataa agttaagata taaacataaa
    27361 gcaaataaat aaaaaagagg tggcaatatg atttatacag tgacattaaa tccatcaata
    27421 gattatatag ttaagctaga tgagctaaag acaggaagta caaatcgtgt aaaagaagaa
    27481 tacgtttatc caggaggaaa aggtataaat gtatctcgca tacttaagga acttggaaat
    27541 gataatacat ctttaggttt tataagtggt tttactggag aatatataat aagaacattg
    27601 gaagaaaaag aattgaagac agattttata aagataaaaa gtggtttttc aagaataaat
    27661 gtaaaaataa aggcgttaaa ggaaactgag attaatggac aaggtccaaa tatagatgat
    27721 gaagacattg atactttata taaaaaactt gataagctaa atcaagatga tattttaata
    27781 ttggcaggaa gtataccatc tactttagat gaaaaactat acgaaaatat aatggctaga
    27841 ttagaaaaaa agaatataaa agttgtagta gatgcaacta aaaatcttct tcttaatgtt
    27901 ttaaaataca aaccttttct aattaagcct aataacgacg aattagaaga actatttggt
    27961 gttaaattaa attcaattga agacatggta aaatatgcta aaagattaaa ggaaatggga
    28021 gctataaatg tcttggtatc aatgggaaaa gatggagcat tgttgattac agaggaagat
    28081 gaagtgctta taagtgatgt ccctaaagga aaagtaaaaa attctgtagg agcaggtgat
    28141 tcaatggtag caggatttat atctgggtat ttgaatactg ggaaatatga ttatgctctt
    28201 aaattgggag cagcttcagg tagtgcaact gctttttcat atgacttagc caaaagagaa
    28261 tacattgata aattggtaaa tgagatatct gtaaagcagt tttaaaatat taaaaaatat
    28321 atagaaaggt aattaatatt tatgaaaatt atagatttat tagatgaaaa gtcaattaag
    28381 ttaaatttaa attcaaaaac caaatcagaa gcaatagaag aattagtaga tttggtcgca
    28441 aatagtggaa atttaaatga taaagaaaac tacaaaaaag caattttggc tcgtgaagaa
    28501 atgagtacaa caggaattgg agaaggtgta gctatacctc atgctaaaaa ttcttcagtt
    28561 actaaagctt gtatagcagc agcagtgtca aaagaaggta tagattatga atcgtttgac
    28621 ggaagtttat caaatctatt ttttatgata gctgcaccag acggagctaa caatactcat
    28681 cttgaagtat tatcaagatt atctacaata cttatggatg aagactttag aaataaactt
    28741 ataaattcaa gctctgaaaa agaatttaag gaaataatag ataaaaagga aagagaaaaa
    28801 ttcagtgaag aatatcaaga tgagaaagtg gctgaaaaaa atataataga tactaaagag
    28861 aatgatgcta ataagtatcc taaggtttta gcagtaacag catgtccaac tggaatagca
    28921 catacattta tggcagctga aagtttaaat aaaatggcag aaaacaaagg tgtatcaata
    28981 aaagtagaaa ctaatggttc agcaggtgtt aaaaataagc ttacaaaaga tgaaatagaa
    29041 aatgcaacat gtataatagt ggcagcagat aaaaatgttg agatggctag atttaatggc
    29101 aaaaaagtca taaaaactaa ggtggctgat ggtatacaca aagcggaaga acttatagac
    29161 aaggctgtaa atggagatgc tccaatttac catggaggag atggaagtca caacgaatca
    29221 aatgaagaaa gtgaaagtgg atttagaaaa gtatacaaac acttaatgaa tggtgtatct
    29281 aatatgttac catttgttat tggtggaggg atacttatag cgatagcctt tttacttgat
    29341 gattatacta ttaacccttc aaactttggt tcaaatacac cgatagcagc gttttttaaa
    29401 gggataggag ataaagcttt tggatttatg ttacctgtac ttgctggata tatagcatac
    29461 tctatatctg atagaccagc gtttgttgta ggatttgtag gaggtgctct agcaggtgat
    29521 ggtggttcag gatttttagg tgcactactt gcaggtttta tagcaggtta tttagtagaa
    29581 gggcttaaga aaatatttag tgtactacca gcttcattgg aaggaataaa accagtattg
    29641 ttataccctt tacttggaac acttttgatg ggaattataa tgacattttt agtaatacct
    29701 ccagttacag caattaataa tgcaatggtt ggatttttaa atggtttagg cggaacttca
    29761 aaaatagttt taggattagt attaggtgga atgatggctg tggatatggg tggacctgta
    29821 aataaagcag catatgtatt tggagttgca tcacttgaat cagggcagtt tgaaataatg
    29881 gcagcagtaa tggctggtgg tatggttccg ccacttgcta tagctcttgc aactacattt
    29941 tttaagaata gatttactaa agaggaaaga gattctggta aagttaatta tgtaatggga
    30001 ttatcatttg ttactgaagg agctatacct tttgcagctg gagacccact tcatgttata
    30061 ccagcttgtg ttggaggttc agcagtggca ggagcactta gtatgttgtt caacgcagct
    30121 cttcgtgcac ctcatggagg agtatttgtt ataccagtgg ttacccatcc atttgcatat
    30181 atattagcta tagctgtagg tgctttggtt ggaatgatgc ttttagcatt acttaaaaaa
    30241 ccattaaatc aagaagtata gcaattaaag gatattcaag aacttaaaat atgatgtttt
    30301 ataaaaatta atatattgct gaattatttt aaaataagga ttttattaaa ataaggattt
    30361 tattaaaata aggattttat gaggctgtta tataacgatt tgtaattgtt atcttgacag
    30421 tcttttattt ttat
//
LOCUS       DAGQWR010000039        30050 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.039, whole genome shotgun sequence.
ACCESSION   DAGQWR010000039 DAGQWR010000000
VERSION     DAGQWR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.039"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            168..413
                     /locus_tag="MIW95_001480"
     CDS             168..413
                     /locus_tag="MIW95_001480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150811.1"
                     /translation="MKKINDNLGFGIAFEEIKSSSKELGMRLPQWSEDVVIKIQRPDE
                     HSKMTASYLYVESRYGRVPWKETMIELFNEKWEVVEI"
     gene            462..719
                     /locus_tag="MIW95_001481"
     CDS             462..719
                     /locus_tag="MIW95_001481"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150812.1"
                     /translation="MNDYKEKTEPKLNILVQSKVDILCSNSSTMNGVDTSVYIKLSLD
                     NRVIGNLTNEDVKYEIIACFNECLEEINRKIGSEEVIDKLQ"
     gene            758..1441
                     /locus_tag="MIW95_001482"
     CDS             758..1441
                     /locus_tag="MIW95_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3150813.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWASE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETQDLINEIKNKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVANLNKDDTDKEITVRV
                     MKASGENGK"
     gene            1431..2783
                     /locus_tag="MIW95_001483"
     CDS             1431..2783
                     /locus_tag="MIW95_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBY3150814.1"
                     /translation="MESKFISNDHFYDFIFNWDYKFYFLVGGYGSSKSYHVATKLIIK
                     LLEEKRKALVVREVYDTMRDSCFSLLEEVAESMGLGDILSFKTSPMRVIFPNGSKIIF
                     KGMDNPAKLKSINGVSIIWLEECSEVKYTGFKELLGRLRHPSLSNHIICSTNPIGEDN
                     WTYKHFFRDEEKDRFVLDDTVLYKNRIVIKNNTYYHHSIADDNFFLPESYIEQLDEMK
                     EYDYDLYRIARKGRFGINGTKVLPQFEVMEHQEVINKISNIPQRYYKVGFDFGFEKSY
                     NALLRLAIDHENKYLYIYWEYYKNQTTDDVTVKDIKEFKDTQELIKADAAEPKTIKYF
                     RQQGFNISGAKKYAGSRLQNTKKVKRFKKIYCSSECKHTIKELKNLTYATDKNGNLIY
                     DEFNIDPHTFSAIWYALDGYEVSDIKELKYNNDIYNKGLGLKKNNIPNQYNKKGGAVF
                     "
     gene            2783..4306
                     /locus_tag="MIW95_001484"
     CDS             2783..4306
                     /locus_tag="MIW95_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBY3150815.1"
                     /translation="MSIKNILLNLDEKELKDRKHAERDFLFYLGECRNKTMGLLDDDF
                     LGQSWITFDNLDYTPSQIIDNKVKPLINKQARFMFGKEPTIILKAYEKEDKETCEELR
                     QYIDSILNASKFWSNTLKAFKIATITKRVLLRLEAEPNQPIRLFYHSIDDFKYQVDSN
                     DITKLKSVIFVRFDASTIKEVTANQIWYRYTYYMKESSVNNQESCFLRVEKFKGDNLS
                     KPIEVKESDTKLSKIPCWIIVNEQSITNITGISDIEDLKPLQDTYNKRLSDFNDSLKF
                     LMFGQTVVVDATEETVNACKIAPNALMALKTLEEGSEKGKQAQAYRVESSFSNADPVD
                     SFFKRLEDSMYEKLAIPRPEQLQNIPSAKALKYLYTELIARCSEKWNDWEPAIRSMLR
                     LIVEACSKFNCYDDWNHDWDDLMFSIVLNKNYPIPEDEEDSKRLALEEVNNNVRSHRN
                     YIKEFGDDEDYEEAFNEVLEDNEKIQSVEQDQFRKDAEIEIDDIDEELNSKSNNSDE"
     gene            4319..5500
                     /locus_tag="MIW95_001485"
     CDS             4319..5500
                     /locus_tag="MIW95_001485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150816.1"
                     /translation="MKDNTYTKKVLEARKKLLLLDKKIQLEILNVYKDASKIILNDIA
                     KNKELNLSTKYLKKLNKSIERYINELNQRLVPVAEKSVIEASNIAKELQMYYYQSIVP
                     NTAMNLACDAMCIKTTTGVVEKLVTGNFYKDKRSLDIRLWEYSSKNRKDIDRLIKANV
                     ARGANAKTLAKSLDNYVNPVRKTEAKTLEVGMNKSISYQAQRLARTSITHAFVETSVQ
                     NAINNPFCAGLQWNLSPQHYIRQVKWRGEDECDEYAEQNRFGLGEGVFPPEKYPIPHP
                     NCLCYPIQVIVPISEASKMMENWIGGGDNDTLDSWFDGIRHQNNSVPIIKKQRNKKNK
                     IATNSKDDRIKKNLTNSIKNKSKKAPKQVQKYINKYVQPSKILIDNSQKYLLFIILRL
                     I"
     gene            5653..5937
                     /locus_tag="MIW95_001486"
     CDS             5653..5937
                     /locus_tag="MIW95_001486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150817.1"
                     /translation="MKNIENLQSIYTNSNELYNNEFISDILGALSNNEFEDLLATHSE
                     KYWSKNRNKEKEIFANLFTLKYQNNKEINSFIKEHLNDLDKIFNELLGGI"
     gene            5939..6091
                     /locus_tag="MIW95_001487"
     CDS             5939..6091
                     /locus_tag="MIW95_001487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150818.1"
                     /translation="MLEEMRKDKKLLELRRLYKEKYGKNAPGFNYDEYNSYAEYKEKL
                     KELIQK"
     gene            6159..6812
                     /locus_tag="MIW95_001488"
     CDS             6159..6812
                     /locus_tag="MIW95_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBY3150819.1"
                     /translation="MLEYFKKLLGDEEGQKVYEKLSKDKENKLLLDNVKSPKYVEKTE
                     LENANKEIKEYKKQIGDRDKQLNDLQGKVKDNKELSDEIESLKNANKEIKENAEKEIE
                     VLKFNTALERAIESYNPKNAKALSALIDKENINFVDGKIIGLDEQIKAYQQSDSYLFN
                     NEKNKGDDENVGTGGLEGGTTSLLDNDNEISSIGELLADSKVKSENIEAQKKFFGEE"
     gene            6824..7180
                     /locus_tag="MIW95_001489"
     CDS             6824..7180
                     /locus_tag="MIW95_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150820.1"
                     /translation="MSIEKSEIYMGENKTILKFAGNLFQNINIKVKKTDVAVVDGKRI
                     LKAGTLISKDGKLVDGTTVTNDKAFGLVYRNIDFTHSNGNESIPVLIFGFVDEKTLPS
                     VIPEEAKQAMKMIMFL"
     gene            7204..8235
                     /locus_tag="MIW95_001490"
     CDS             7204..8235
                     /locus_tag="MIW95_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBY3150821.1"
                     /translation="MDWKDFIDSKEIAKYIKKLPLEMLIGESLFPRKKQIGMDLKYIK
                     GAKKKPVVLKQSTFDVAVKIRALKAQIEVKSKRMPFFKESVLVNEEDRQQLLLASKAQ
                     NKELLLMIISQIYDNYLALVDGGDMQMERMRMQALADGVINIVSEDGDLVFDFEVPSN
                     HKEVLTGSATWDNPDADIIGDIQRWMRIMRDEGKPLPKRMVMTSKTFGYFAKNKAIKL
                     DIDKDGRVILTDEMIKNYLKNKVGLSVAIVSGTYKLEDESEESYFPDNKITFIPDGDL
                     GKTYYGTTPEEADKVYGSKLDCSVVRTGIAITTMRLIDPVTVQTKVSQLGMPSFERAD
                     ECFFATVAS"
     gene            8250..8450
                     /locus_tag="MIW95_001491"
     CDS             8250..8450
                     /locus_tag="MIW95_001491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150822.1"
                     /translation="MAKKKENLMQVKALVYLKYDNDCYKIDDIFEIRKADQELMEEKG
                     YIEVIGEAEEKNNNEILNKDGE"
     gene            8453..8770
                     /locus_tag="MIW95_001492"
     CDS             8453..8770
                     /locus_tag="MIW95_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150823.1"
                     /translation="MAINNLDKLKLNLQEEEYPYFTDEQLVMLLESNENNVLKASWRG
                     CLLKGATDDCIKIGPIETKSSNSSYWLSLADIYKTDYLEEKSKNETTNTGYKTSMTRV
                     DGQ"
     gene            8767..9150
                     /locus_tag="MIW95_001493"
     CDS             8767..9150
                     /locus_tag="MIW95_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150824.1"
                     /translation="MRKLRADKIIKTINRGIALNPQIITIEQEIKKIVDGAIEVTNEV
                     KELTVVIYPEKSNDTVVSSETIGTVYKNKNFGMVADKEADLRLNTENEITFKCIEGTM
                     KLNYVNPIVVEEKICGYICGLEKID"
     gene            9169..9573
                     /locus_tag="MIW95_001494"
     CDS             9169..9573
                     /locus_tag="MIW95_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150825.1"
                     /translation="MFTKAINEIDRKKATMPILCTNIAFMLEGEAKNNAKWTDRTGNA
                     RQGITGTSLGGGNQYIVRLGHGVDYGTVLEEGSAPHVIKPKSAKALFWNGASHPVKQV
                     QHPGTKGTHSLESTVSRNISKIGKLVEGHWSK"
     gene            9575..10399
                     /locus_tag="MIW95_001495"
     CDS             9575..10399
                     /locus_tag="MIW95_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150826.1"
                     /translation="MRAGIRKALIDNIPRLKDCYEPTVPNKKTIKPYIVVVQGEDADN
                     EGDVIGFRRTINIWLYEKRTTFNKLDELTKEVIETLDFKTITDDTSNEVFTCIYEGAV
                     GQDVIDEEWEAIIRCLRFSVIALDDKEDITNDRWVEALSKYTKNLLEIESYKDNWKKN
                     FIAPCALWRTTNIENKRINYHLIEITKTMKCHVVSKNKDEIVKLLEELETKLIIDKRV
                     RLREDKNMYLTLVSVVEDRESDMFSNGQLTVVFKMIGKIKREGPIMNKIYSNGNLK"
     gene            10412..10615
                     /locus_tag="MIW95_001496"
     CDS             10412..10615
                     /locus_tag="MIW95_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150827.1"
                     /translation="MAEAINKKTNISKQEEKYSKEDFLKNSEALGYKKEVVAGALFNC
                     KKDELTKVEFEKLIKDFLEREVK"
     gene            10617..12038
                     /locus_tag="MIW95_001497"
     CDS             10617..12038
                     /locus_tag="MIW95_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBY3150828.1"
                     /translation="MATGAWNEKEKKDIPGFYNRFKTQAEKSTNTGLKGRLAMPIRAN
                     WGDVGKVITIKNDLRQLKTLFGDDMNYSAYKLGKLALLGNVKELLLYRLVDGNQKKGT
                     LTLKDTTENTAKDVIKLETKYPTSRNFNVTIKSNLVDSDKKDFIFFEGTKQLFSSSVK
                     GTIDEIVLEINSNLDNEYVIATKVADSDTTLANLVNVALEGGNDGCTSITNESYLKAL
                     EEFERYSFDSFVLDGVAEEALQETTKAWVAKNKELGKGILLFLGGKKEDNIKQINDKS
                     KSFNDENIANVGSSAYYENVKYTPSEVAVYIGALAVSKGITGSICNAKTIFEEVEPRL
                     SQSEVKECLKSGTLVLDFDDGDVIVVDDVNTFKKYVDDKNEAMGYISNIMFINTINKD
                     TSLKRKEFVGKIFNDATGQTTVICALKKYFEELMSQGIISEFNVDIDTELQATAKADE
                     FYWKWDAIKVDVMKKIYGTGYLG"
     gene            12054..12467
                     /locus_tag="MIW95_001498"
     CDS             12054..12467
                     /locus_tag="MIW95_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150829.1"
                     /translation="MYNDDYIEEASFLNGSDVVILIDGVEELYMEEIKADFEQDEQSI
                     KLLGCQNEISRVGTTKGSFSLSGYKVSSKFAKLGFKSFEIIYKLTNPQTLGYESIRLK
                     NCRLKKIPLINSKAGEDVKIELEGSYRGYDLLNEL"
     gene            12550..13038
                     /locus_tag="MIW95_001499"
     CDS             12550..13038
                     /locus_tag="MIW95_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150830.1"
                     /translation="MSEIYKRELENEVEDNDLDEEIEETNEDRLKMKEDEIIAKLLED
                     SPVPERTVFLDRLGIPITLKALTEKEISKIRKDCTKIVKVQGRREEKLNDDEFTLALI
                     EKGTVKPNFSNQKLLDAMKVTNAREFIKRKFLAGELSKISDQILELSGFYDEISDDDI
                     KN"
     gene            13044..13196
                     /locus_tag="MIW95_001500"
     CDS             13044..13196
                     /locus_tag="MIW95_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150831.1"
                     /translation="MGGRLTVLNNIFVKHHVPPDIFVKKNLMSQRLMKVFTQNEIEQE
                     NKAMKK"
     gene            13215..17492
                     /locus_tag="MIW95_001501"
     CDS             13215..17492
                     /locus_tag="MIW95_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBY3150832.1"
                     /translation="MREIAKKEMYHIDVVISAKGDGETKSKLSTMEKYMKQTEKRMQT
                     LNRIKVNPAIKATDKASSVVNRVNNNMNKAKKTVTARIKATDNASPVANRASNNVNKA
                     KKTVTARLKATDNASSTVNKVNNKIKEVAKPVPPVIIRGQDESSSIIDKVKAKIQNLK
                     ADTIIKIKSQADEAINTISRTKNKLQEFVSKRYEAAVKIRDEASSALGGLTGKIDSFV
                     NGAISKFARLATTAGALIGGIGVGSAVKGFATFEQSMKNTQAVSGATGKEMEALTAKA
                     RQLGRETSFTAKDAGDAMYYMGMAGWKSEQMIKAIPDVLNLAAAGGTDLALTSDIVTD
                     GLTALGMTANDTTEFVDVMAATITNSNTSVELMGETFKYVGSMGGALGVSMKDLSLAT
                     GLMASASVKGSMAGTSLRGGLVRLIKPPEEAASAIKKYGIELKKNKNGSLDLAGTIGS
                     LREKLGGLEDVEKGVAISSIFGRTAMAGWAAVVNASESDFNKLTTAIAESEGEAKRIA
                     DMKLDTLSGQFEILKSAIDDVRISVGSKLGPMTRQFVEKLIKDMPKIGDAIVGVVEKF
                     VNNFDKIKAGFQVLLPAIGSVIASVMVLKATFAFGGAIKSLSLLANTFGTAKLAAFGL
                     TVGIGAIVIAFAGMTVAISNNKTAMMDLQTRFGSFGEYVTTIMETVGGVIKLTLGNLL
                     IMLGGIGKGIGILLSDKSWDEKASSLKNLFGKTTAEIKTNTKEALSDINGETSNATAL
                     LKKSTSKELQGVTKAFSTAFDQSKNVTEKKSNDIARALTNGLKGLDDQSLTMMRGLND
                     NMAIILSGVTADMKPSDKVNKITKNLDDAFKAGKLSAQEYRSSIQETLSFISKYSADS
                     SNNLKQGMSDAFNAFKEGTNVSGLKDGVTGMLNSLKATGPQALETLKGLGGKASEIFK
                     GVDFNSSIDAQKTKVLQNLNNLGLEGTQAIDTLRTIFSQASTALDTTNLKQGLSDTFN
                     SFKIGFNQDGINGAISNMLGTINQIGPQMQQTLGKMDGEMGQIFANVDFSTPIETQAS
                     KVLENLNNLGIEGPKALETVRSIFAQASSQIQGSASQTAQQANQQVANALTQENPAVQ
                     QAGQQLGTDLTNGVVNGVQTGVPTVQQKGNELGVATQQGVTDALNNATPQINNNNLTT
                     GIDTAFNQATATVQQGATNMYNGAKQSFSQLAQVGREAGSSLYNGATTSFNMLASNVR
                     VACSSMYNGARTSFTSLSSSAISAISAMCSSVVSQVSAMSSQIISYWNSVRATVSAPI
                     SASFNVKTTQTTVKRTVNEGGGILSNIFGRFADGGVASKPSICGEDGAEMVIPLSNNR
                     RNRAIGLYEQTGKMLGLSSTPSQKIGESYSNVVNNVRQFPLTNVLDTDNKEYREATPN
                     SVNSSNSTINLGGISINVQESNNKEEMIQEILSQVEDGLREALQDIG"
     gene            17574..18374
                     /locus_tag="MIW95_001502"
     CDS             17574..18374
                     /locus_tag="MIW95_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150833.1"
                     /translation="MWQKFKSLNTFLKVIIGIIAICFLPITLAVFSIEFLMKAIEAKN
                     KGKIVLGCFLALLTLSCARTVYLTKDLDDKNSTQVNQVSSNKDKEDAEKKAEEETKKQ
                     KEQKAQTEKQKKDEEAIKLVEQQKKNEDEQKSLENKKNNEPTKFFNDVYLKNKNCVNM
                     ASYEGIKSNIDIFGYKYKIIEPNETTLRQIDVYDNQSNDTVQFQFYENDTGEEVVTVM
                     QYNKKDEPDKSVAISDDFHIYPPKFNNHINNTNTEVNSIEEQINFMFN"
     gene            18443..19114
                     /locus_tag="MIW95_001503"
     CDS             18443..19114
                     /locus_tag="MIW95_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBY3150834.1"
                     /translation="MVIDIYLKNEKEKIDFHFPVTPLDSLSIKKEKRFETVDIVNLGE
                     FDIKKEGEKIREISFKTFLPFQYDASYCRYSELKNPTEIVAMLEKWVDQSEPLRLIMT
                     GFGYNGLVTISSFSNTQTAGREEDRDIEITFRTYRELKIETLKKETKSNTKTDLKDNR
                     PNNKTTTKIYTVKASDTLYKIAKNLLGKGSRWPEIYNIPENKKTIGKNPNVIKKGQKL
                     VIPSK"
     gene            19111..20073
                     /locus_tag="MIW95_001504"
     CDS             19111..20073
                     /locus_tag="MIW95_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ"
                     /protein_id="HBY3150835.1"
                     /translation="MKIILNGKYDIANFNEGITLSEAIDGVAYKMDVSLVEPKQLQDI
                     GIKKGDKIVLIDIAYESKKEETIFNGVVWETRRSEKSKKLTLSCRERTVYMEESEEQY
                     QFKENTATQRIEYYCKQWNIPCYNLANTGKKLAKVIHKTNILDMIKKDLKETASKGGD
                     LFRVRMDNKLKLFKLGTNANVYKLDSILEDANFTSSFNDAVTSVKVLGKSKDENTKAP
                     IIGTYKKDSDKYGTLQKIKQDEKIKNAKEAKKAAEAMFNSGEETISVDCAVDINRIRA
                     GDKVSLKGKEYYVIDVTHTLDSIPKMKLNIGTLDYIRRKFYNND"
     gene            20066..20431
                     /locus_tag="MIW95_001505"
     CDS             20066..20431
                     /locus_tag="MIW95_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150836.1"
                     /translation="MTDARFNRVARILKEKMNKSVNDGVFGMGCSLAEITTNGLKVNG
                     YKDEIQDYLVLENLTLKEDYFTLSDEILGAEYRHKHKIETPKELKPLTIGDNVLVAVM
                     GTEFVVIGRVVNAKPISTK"
     gene            20406..20864
                     /locus_tag="MIW95_001506"
     CDS             20406..20864
                     /locus_tag="MIW95_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBY3150837.1"
                     /translation="MPNLFPQNETFETVELKNNNENELDLKGSFLFDFIKGEFVKNAD
                     GTLKRCNKVEAYKQWCQKAILTPRYKRSAYSSVYGSEIKDLIASNLSQSAKELEISRL
                     IKETILVHPYTKEVSNFIFNWLENSRLVNYEFDVLTIDDENIVIDGNIKR"
     gene            20873..22009
                     /locus_tag="MIW95_001507"
     CDS             20873..22009
                     /locus_tag="MIW95_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBY3150838.1"
                     /translation="MERELPIPVFLTEDEEVIHERMLSNFQDVSTLEGDFIYDATRPT
                     AEEITQLKQLGLQNNLKIAFPQTSYGTYLEWLGECKGVFKNQPTKSVGMVTFTGVQGA
                     IIAKGTIVTTIATDEKQSIEFELLETKTIGENETVDIKAECRIAGIIGNVSKGSISVL
                     LGSINGIKSVNNKEDFKGGTDIEDEEHFRERVLVSEQEDRLSGASSDYIRWAKEVDGV
                     GYAYVVSEWAGAGTVKVLILDKNRKAATQELIDKVQEYIYPLNVSEGENRDGKAPIGA
                     LVTVVTPETLLINVKASFIFSNSFSEETVLNNLKAKIDKYLDKIDLGGTVSYNAIQAI
                     VGSMMLTDEGIQDFSNLTINDVKENIKLQDQVVGVGEIVNEVVG"
     gene            22006..22653
                     /locus_tag="MIW95_001508"
     CDS             22006..22653
                     /locus_tag="MIW95_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBY3150839.1"
                     /translation="MIASKKGREMLLTLSPIYEQSIIMQSLYEAIGSEFDNLDLLNKE
                     IELQLFPQSATWGLEFWENRVGLSTNLDEAIEARRRKVIAKLQSKYIMTPKRMSMILQ
                     SYTGANIKINENISPYTFGVELTSTQGFPRDLEDLYKRVNVIKPSHLSVSYKLVSLLK
                     SKTYFAQTTIMSEEITVYPYSSKEVKANIKAKFALAHNMSSEILTVYPKGGGING"
     gene            22646..23446
                     /locus_tag="MIW95_001509"
     CDS             22646..23446
                     /locus_tag="MIW95_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBY3150840.1"
                     /translation="MADEQFYTILTNIGKAKIANAGMLGKSVVLEKIQAGDGGGSYYN
                     PTEDQTALKNKVWEGNINVFDKDENNPNWIIATACVPGSIGGFTVREMALIDNEGDMI
                     AICKSPETYKPKVGNGAMKDLYLKFIIEVSNVEKVTLVVDPTAIFLTKKDEEKILTNI
                     NKLDTKIDTTKTELTSNIETAKTELNNKIGDTTQLTTTDKTNIVSALNEVKTSVDSIE
                     TTAEKTSYNNATSNLTATNVQGAIDEIVAKIENFNEINISIQNDMLPI"
     gene            23463..25181
                     /locus_tag="MIW95_001510"
     CDS             23463..25181
                     /locus_tag="MIW95_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150841.1"
                     /translation="MAIVYEFNYTGAEQSVVLPPGKYKFECFGACGGNYYDFVQCAKG
                     GYTAGSLILKENTTLHVYVGQSGYCKGVNGIETCRSGFNGAGGITTYKSTSDGYYSLA
                     GGGATDIRLIGGNWDNLQSLLSRIIVAGGGGGGSGNSHDSIGHGGGTKGKDGISIANK
                     YFAGGGSQFQGGLTFNSLYNGSFGVSGAGDGISGVGGGGGWYCGAGSFYAEFGGGGSG
                     YILTKDSYKPANYSPSSKYYFSDINSVVGGNTTKQDGYAKITLLQALPFLTISSYNST
                     TATFKADHTDPTLLTKIEYFIDDVLKETITTDLTTEKIINYTLEDNALHTLKIVVTDS
                     ANATAEKVVSISKGIAPLPAGSTTDEVTSKWIEIKDTFKSGKTSIINTLALKNIEASL
                     NNTLVELSEKIKTSFDSSDASVQDLMNQLTQANNTITQLNTKYKVASGTVTPFKGDDT
                     KIAYPYLTDRVFKPSAWVKISNLDFKPNIFFADFDYYDTEYKNNYKLFLFACCGVATQ
                     RGVDFSSVTSFVRKSSDEYFHANGWLYNNSEGDVYFNNTGVQIPAYSFDSTQRHIYKW
                     YAIKFI"
     gene            25196..25492
                     /locus_tag="MIW95_001511"
     CDS             25196..25492
                     /locus_tag="MIW95_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150842.1"
                     /translation="MNVPNRVIYDQTGRIIFETGESCGDVLPHDKITELHYIDVEYGS
                     IDYTRNKIVGINIETKEPVLEEIPAYISKAEKEKQELENQLLLMTNKELGGGIL"
     gene            25492..25656
                     /locus_tag="MIW95_001512"
     CDS             25492..25656
                     /locus_tag="MIW95_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150843.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNRPFELDDVTNIEYRKAVDD
                     YINTIEQEIK"
     gene            25854..26465
                     /locus_tag="MIW95_001513"
     CDS             25854..26465
                     /locus_tag="MIW95_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150844.1"
                     /translation="MIEKLNENASLSDLITAFENSTKELKASKDNFTNLLGNPFLEST
                     KFSEFEGKMQNLMSTFKDNLKSKGINSGNTESLLSLINKVANIYIPTPIYTASGECTI
                     DNKGTCKRYTISTAGDVYYVRINLNFYPKFIVMYNSGGASKYFTTSYFCSYVPFIAST
                     VESYTTYLLDKSDSRIQVQKGTCILPIVYSKYGSSSFYFHAVG"
     gene            26477..26782
                     /locus_tag="MIW95_001514"
     CDS             26477..26782
                     /locus_tag="MIW95_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150845.1"
                     /translation="MNDYIEVGRRIFFDEEGEIIFYEGQSKGNVPERKNIKKIEYIDL
                     EYDYVDYDKYKIIGINIETKQPILEEIPIYMSEEEKRIQELENQLLIAENEKVGGLL"
     gene            26924..27187
                     /locus_tag="MIW95_001515"
     CDS             26924..27187
                     /locus_tag="MIW95_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150846.1"
                     /translation="MEQLLTELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVEMGAMKTDVEEIKEDVTDIKDMLQKEGL"
     gene            27188..27985
                     /locus_tag="MIW95_001516"
     CDS             27188..27985
                     /locus_tag="MIW95_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY3150847.1"
                     /translation="MKVAIVPGHTLTGKGTGATGYINEGTENRILMDLVVKWLKKGGA
                     TVYSGKVDKSNNYLAEQCQIANKQNVDVAVQIHFNANKTTLNPIGTETIYKTNNGKVY
                     ANRVNDKLATVFKNRGAKSDVRGLYWLSHTKAPAILIETCFVDSKADTDYYIKNKNTV
                     AKLIAEGILNKKIDNIEVKQMYKHTVVYEGEVDKVLAQVISWNYKENECRVCDIKDYV
                     PGQTQNLYIVGGGACDKIGSITKERYTMIKGNDRFDTLYKALDFINR"
     gene            28093..28380
                     /locus_tag="MIW95_001517"
     CDS             28093..28380
                     /locus_tag="MIW95_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150848.1"
                     /translation="MLINDKSFYPNNIYPAIDFLKIKRQLKSIYKNDLSDCGSICIIE
                     RKEYSISINSIGEINIYYDSRYENKIQNVVDEIEQLFKSQVKNFSVSKFKN"
     gene            complement(28428..28640)
                     /locus_tag="MIW95_001518"
     CDS             complement(28428..28640)
                     /locus_tag="MIW95_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150849.1"
                     /translation="MTWKNEIKSILVKENISINELNNLMNEHNNKNNTVENLRQKINN
                     ETMKYSEILTIASLIGYEVVWKKKEK"
     gene            28821..29270
                     /locus_tag="MIW95_001519"
     CDS             28821..29270
                     /locus_tag="MIW95_001519"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150850.1"
                     /translation="MEKFIRLDFDKGFRGKEHRSSATGDGEHFEAGISCYKISKEKCV
                     DAIINLCEYWFKIAGECQFKDYDINIFEGYCVGEGCSYEDLATCENHLYCIDGSLFNE
                     VYDLYYKHDTYLDEDGDIEELEENYKDEYITTEEFESKIKEMFIKYL"
     gene            29291..29956
                     /locus_tag="MIW95_001520"
     CDS             29291..29956
                     /locus_tag="MIW95_001520"
                     /inference="COORDINATES: protein motif:HMM:NF013043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150851.1"
                     /translation="MNIGDKFGNFTILDIEQRNKRKYYLCKCSNCNNEKWIRADSLKR
                     IKACGCMQSETQFKQNNLAGKKFGRLTAIKSTNKKTKSGHYIWICKCDCGNEIETAEN
                     NLTTNRTKSCGCLKKEAEIRNAKIALRVHQQKNIVDDTNISIIKKTEASINSKTKIRG
                     VCWNKEKGKYHAQIEFKKVHRNLGYYDNIEDAEKAYMEAKEKLHKKFLKDNDKLKEIE
                     YQM"
BASE COUNT        12415 a         3295 c         5718 g         8622 t
ORIGIN      
        1 tttggtaaaa gaagtcttga taaagatgaa acagattcat tggatgggga ctatctagtt
       61 caaatgttaa aatatctaaa aaaataaaag tacataaaat ctaaaatgac tatctttact
      121 ttatagatgg tcttttttta tacaataaat taaaaggaga aaagaaaatg aaaaaaataa
      181 atgataattt aggttttgga attgcttttg aggaaataaa gagtagtagc aaagaactag
      241 gtatgagatt accacaatgg agcgaggatg ttgttataaa gatacaaaga ccagacgaac
      301 atagcaaaat gacagcatct tatttatatg tggaaagtag atatggtaga gttccttgga
      361 aagaaacaat gatagaacta tttaatgaaa aatgggaagt agtagaaata taaaatatat
      421 tataagttat gggaagtaat aagataaaag gagaataaat tatgaatgat tataaagaaa
      481 aaactgaacc aaaattaaat attttagtcc agtctaaagt tgatatttta tgttctaata
      541 gctctactat gaatggagta gacacttcag tttatataaa actttcttta gataatagag
      601 ttattggtaa cttaaccaat gaagacgtga aatatgaaat aatagcatgc tttaatgaat
      661 gtttagagga gattaataga aaaattggaa gtgaagaagt aatcgataaa ttgcaataat
      721 ctttattccc aaaacgacaa acaaacgagg tggtgatgtg caagatgtca aagaaaaggt
      781 aaaacaagat tacctaaaag gtatgaaaca aaaggaaata tcagcaaagt atgacattag
      841 tttaaatact ttaaagtcat ggattaaaag atacaactgg gctagtgaaa aaaagaaggg
      901 tgcacctaaa aataaaaggg gtgcacccat aggtaataag aatgccactg gacctcctgg
      961 taataagaac gctgaaaagt ttggtttctt ctcaaaatat ctacccgaag agactcaaga
     1021 cctaattaat gagataaaga ataaagataa attcgatatt ctttgggagc agataacaat
     1081 tcaatatgca gcaataataa gagcacaaaa gataatgtat gttaaagata aagaagaaat
     1141 gattaaggaa cttaagaaac atgaaagtac agaaaatggc gagaagatag agtatgaatt
     1201 tcaatttgca tgggataggc aagcatcttt tcttaatgca cagagtaggg caatgagtga
     1261 gttaaggagt cttataaagc agtatgatga aatgattcat aaggattgga atttggctac
     1321 agaggagcaa aaaactagag ttgagaagtt gaaatgtgag gttgctaacc taaataaaga
     1381 tgatacagat aaagagataa ctgtaagagt tatgaaagcg agtggagaaa atggaaagta
     1441 aatttatatc aaatgaccac ttttatgatt ttatattcaa ttgggattac aaattttatt
     1501 ttcttgttgg aggatatgga agttcaaaga gttatcatgt agcaactaaa ttaattatta
     1561 agcttcttga agaaaaaaga aaagctttag tggtaagaga agtatatgac acaatgagag
     1621 atagttgttt ttctctatta gaagaagttg cagaaagtat gggtttagga gatatattat
     1681 catttaaaac aagtcctatg agagttatat ttccaaacgg aagtaaaata atatttaaag
     1741 ggatggataa tccagctaaa ttaaagtcaa taaatggtgt atctataata tggttggaag
     1801 aatgttcaga ggttaaatac actggtttta aagaactctt aggacgtttg agacatccta
     1861 gtttatctaa tcatataatt tgttcaacta atccaattgg cgaagataac tggacctata
     1921 aacatttctt tagagatgaa gaaaaagaca ggtttgtatt agatgatact gtgttatata
     1981 aaaatagaat agtaataaaa aataatacat attatcatca ttcaattgca gatgataatt
     2041 tttttctgcc cgaaagttat atagagcaac ttgatgaaat gaaagaatat gactatgact
     2101 tatacagaat cgcaagaaaa ggaagatttg gtataaatgg aactaaagta ttaccacaat
     2161 ttgaagttat ggagcatcaa gaagttataa ataaaatatc taacatacca caaaggtatt
     2221 ataaagttgg attcgatttt ggatttgaaa aatcatacaa tgcactttta agattagcta
     2281 tagaccatga aaacaaatat ttgtatatct attgggagta ctataagaat caaactacag
     2341 atgatgtgac tgttaaagat attaaagagt ttaaagatac tcaagaacta ataaaagctg
     2401 atgctgctga acctaagact ataaaatatt ttagacaaca aggttttaat atatctggtg
     2461 ctaagaaata cgctggaagt agactacaaa atactaagaa ggttaagaga tttaaaaaga
     2521 tttattgttc aagtgagtgt aaacatacaa taaaagagtt aaagaaccta acatacgcta
     2581 cagataaaaa tggaaactta atatatgatg aatttaatat agaccctcat acatttagtg
     2641 ctatatggta tgcacttgat ggttatgaag tgtcagatat taaggagtta aaatataata
     2701 atgatattta caataaaggc ttaggcttga agaaaaataa tataccaaat caatataata
     2761 agaagggagg ggctgtattt tagtgagtat aaaaaatatc ttgcttaatt tggatgaaaa
     2821 agaattaaaa gatagaaaac atgcagaaag agatttcttg ttttatttag gtgaatgtag
     2881 aaataaaaca atgggattat tagatgatga ttttctaggt caaagttgga ttacttttga
     2941 taatttagac tatacacctt ctcagattat agataataag gtaaaaccac ttataaataa
     3001 acaagctcgt tttatgtttg gaaaagaacc aactataata cttaaagcat atgagaaaga
     3061 agataaagaa acatgtgagg aattaagaca atatattgat tcaatattaa atgccagtaa
     3121 gttttggagc aacacactaa aagcttttaa gatagcaact ataacaaaaa gagttttatt
     3181 aagattagaa gctgaaccaa atcaacctat aaggctattt tatcattcta tagatgattt
     3241 taaatatcaa gttgatagta atgacattac aaaattaaaa tctgttatat ttgttagatt
     3301 tgatgcttca accataaaag aagtaacagc gaaccaaata tggtatagat atacttatta
     3361 catgaaagaa agtagtgtta ataaccaaga aagttgtttt ttaagagtag aaaaatttaa
     3421 aggtgataat ttatctaagc ctattgaagt aaaagaaagt gataccaagc tttctaaaat
     3481 accatgttgg ataattgtta atgaacaaag tattacaaat atcacaggca ttagtgatat
     3541 tgaggattta aagccattac aagatactta taataaaaga ttatcagatt ttaatgactc
     3601 tttaaaattt ctaatgtttg ggcaaactgt tgtagtagat gcgacagaag aaacagttaa
     3661 tgcctgtaag attgctccga atgctttaat ggcattaaaa acacttgaag aaggttcaga
     3721 aaaaggaaaa caagcacaag catatagagt tgagagtagt ttttcaaatg cagacccagt
     3781 cgactccttt tttaaaagac ttgaggatag tatgtacgaa aaacttgcaa tccctagacc
     3841 agagcaatta cagaatatac ctagtgccaa agctttgaaa tatttatata cggaacttat
     3901 tgcaaggtgt tcggaaaagt ggaatgattg ggaacctgca ataagaagta tgttaagact
     3961 aatcgttgaa gcatgtagta aatttaattg ttatgatgat tggaatcatg attgggatga
     4021 tttaatgttt tctattgtat taaataaaaa ttatcctatt ccagaagatg aagaagattc
     4081 gaagagattg gcacttgaag aagtgaacaa taatgttaga agtcatagaa attatataaa
     4141 agaatttggt gatgatgaag attatgagga agcatttaat gaagtattgg aagataatga
     4201 aaagatacaa tcagtagagc aagaccaatt cagaaaagac gcagaaatag aaattgatga
     4261 tattgatgaa gaattaaata gtaaatctaa taatagtgat gagtaatgag gtgttgttat
     4321 gaaggacaat acttatacaa agaaagttct tgaagctaga aaaaaacttt tattattaga
     4381 taaaaaaata caattagaga tattaaatgt atataaagat gccagtaaaa tcattttaaa
     4441 tgacattgct aagaataaag aattaaattt aagtaccaaa tacctaaaaa agctaaataa
     4501 atcaatcgag aggtatatta atgagttaaa ccaaagatta gtacctgttg cagaaaaaag
     4561 tgtaatagaa gcttctaata tagctaaaga attacaaatg tattactatc aatcaatagt
     4621 tccaaataca gctatgaacc ttgcatgtga tgctatgtgt ataaagacaa ctactggcgt
     4681 ggtggaaaaa ctagttacag gtaactttta taaagataaa aggtcattag atattaggct
     4741 ttgggaatat agtagtaaaa atagaaaaga cattgataga ttaataaaag ctaatgttgc
     4801 tagaggtgct aatgcaaaaa ctttagcaaa gagtttagat aattatgtta atccagttag
     4861 aaaaacagag gctaaaacat tagaagttgg aatgaataaa agtatatctt atcaagctca
     4921 aagacttgct agaacctcta taacacatgc ttttgtagaa acaagtgttc aaaatgcgat
     4981 aaataatcct ttctgcgcgg ggttacaatg gaacctaagt cctcaacatt atattagaca
     5041 agtaaagtgg aggggcgagg atgaatgtga cgagtatgca gaacaaaatc gttttggact
     5101 aggtgaggga gttttcccac ccgaaaagta tcctattcca caccctaatt gtttatgtta
     5161 tccaattcaa gtgattgtac caattagtga agcttctaaa atgatggaga actggatagg
     5221 tggaggagat aatgatactc ttgatagttg gtttgatgga ataagacatc aaaataatag
     5281 tgttccaatc attaaaaaac aaagaaataa aaagaataaa atagctacaa atagtaaaga
     5341 tgatagaatt aaaaagaacc ttactaattc aattaagaat aagtcaaaga aagcacctaa
     5401 acaagttcaa aaatatatta acaagtatgt gcaaccaagc aaaatactaa ttgataatag
     5461 tcaaaaatac cttttattta ttatactaag gttgatttaa ttggtattaa tccaaatatt
     5521 aaagaattta aatcatataa taaagaagct gctttacttc atgagtttgc gcatagaatt
     5581 gatataaaag aaattaagag ctataataat attaagttcc aaaaagctat tgaaagtagc
     5641 tctatatatg ttatgaagaa tattgaaaac ttgcaaagta tatatactaa ttctaatgag
     5701 ttgtacaata atgaatttat tagtgatata ctaggagcat tatctaataa tgaatttgag
     5761 gatttattag caactcatag cgaaaaatat tggagtaaaa acagaaataa agagaaagaa
     5821 atatttgcta atttatttac actaaagtat caaaataata aagaaataaa tagttttatt
     5881 aaagaacatt taaatgattt ggataaaata tttaatgaat tattaggagg gatttagatt
     5941 gcttgaagaa atgagaaaag ataaaaagct gttagagttg agacgattat ataaagaaaa
     6001 atatggtaaa aatgcacctg gtttcaacta tgatgaatac aacagttatg ctgagtacaa
     6061 agaaaagcta aaagaattga tacaaaagta aagtaagcac ttattaatta aaggttaatg
     6121 agtgctttta ttatgcttaa ttttaaggag gaaattgaat gttagagtat tttaaaaagt
     6181 tacttggaga tgaagaagga caaaaagttt atgaaaaatt atctaaagat aaagaaaata
     6241 agcttctttt agataacgtt aaaagcccta aatacgttga aaagacagaa ctagaaaatg
     6301 ctaacaaaga gattaaagag tataaaaagc aaatagggga tagagataag caattaaatg
     6361 acttgcaagg taaagttaaa gataacaaag agctatcaga tgaaattgaa agtcttaaaa
     6421 atgcaaataa agaaattaaa gaaaatgcag aaaaagaaat tgaggtttta aaattcaata
     6481 cagctcttga aagggctatt gaaagttata atcctaaaaa tgcaaaagct ttatcagcat
     6541 taatagataa ggaaaatatc aactttgtag atggcaaaat tattggatta gatgaacaga
     6601 taaaagctta tcaacaaagt gattcttatt tatttaataa tgaaaaaaat aaaggagatg
     6661 atgaaaatgt aggaactgga ggactagaag gaggaacaac atcattactt gataatgata
     6721 acgaaataag tagtataggt gagttgctag ctgactctaa agtaaaaagt gaaaatatag
     6781 aagctcaaaa gaaattcttt ggagaagaat aggaggaaaa tatatgagta tagaaaaatc
     6841 tgagatttac atgggggaaa ataaaaccat attaaaattt gcaggaaatt tatttcaaaa
     6901 tataaatatt aaagtgaaaa aaactgatgt agctgtagta gatggaaaaa gaattttaaa
     6961 agcaggtaca ttaatatcaa aagatggaaa attagtagat ggtacaactg ttacaaatga
     7021 taaagctttt ggcctagtat atagaaatat agattttact cattcaaatg gtaatgaaag
     7081 tattccagta ctaatatttg gatttgtgga tgaaaagacc ttaccatcag ttataccaga
     7141 agaagcaaaa caagcaatga aaatgattat gtttttataa aataaatatt aggaggaatt
     7201 aaaatggatt ggaaagactt tatagactct aaggagatag ctaaatatat aaaaaagtta
     7261 ccactagaaa tgctaatagg cgaatctttg tttcctagaa aaaaacaaat aggtatggac
     7321 ctaaaatata ttaagggagc gaagaaaaaa ccagtggtgt taaaacaaag tacttttgat
     7381 gttgctgtaa aaataagagc attaaaagct caaatagaag ttaaatcaaa gagaatgcca
     7441 ttttttaaag agagtgttct tgtaaatgaa gaagatagac aacagttact tttagcttct
     7501 aaagctcaaa acaaagaact attattaatg ataatttcac aaatatatga taattattta
     7561 gcccttgtag atggtggcga tatgcaaatg gaaagaatga gaatgcaagc tctagctgat
     7621 ggagtaataa atatagtatc agaagatgga gatttagtat ttgactttga agttccaagt
     7681 aatcataaag aagtgttaac tggaagtgca acatgggata atccagatgc agatattata
     7741 ggagatattc aaagatggat gagaataatg agagatgaag ggaagccgtt acctaagcga
     7801 atggtaatga caagtaaaac ctttgggtat tttgctaaaa acaaagctat taaattggat
     7861 atagataaag atggtagagt tattttaact gatgaaatga ttaaaaatta tcttaaaaat
     7921 aaagttggtt tatctgttgc catagtaagt ggaacatata aattagaaga tgaaagtgag
     7981 gaatcttatt tccctgacaa caaaataact tttattccag acggagattt aggaaaaact
     8041 tattatggca caacaccaga agaagctgac aaagtatatg gttctaaatt agattgttct
     8101 gttgttagaa ctggtattgc tataacaaca atgagattaa ttgacccagt aacagttcaa
     8161 actaaggtat cacaattagg tatgccaagc tttgaacgtg ctgatgaatg tttcttcgct
     8221 acagtagcat cttaaaaaag gaggatatta tggctaaaaa gaaagagaat ttaatgcaag
     8281 taaaagcttt agtttactta aaatatgata atgactgtta caaaatagat gatatatttg
     8341 agattagaaa agctgaccaa gaacttatgg aggaaaaagg atatatagaa gtaataggag
     8401 aagctgagga aaagaataat aatgagattc tcaacaaaga tggtgagtag acatggctat
     8461 taacaattta gataaattaa aactcaattt gcaagaagaa gagtatcctt attttacaga
     8521 tgaacaactt gtgatgttat tagaatctaa tgagaataat gttttaaaag cttcatggag
     8581 aggttgttta ctcaaaggtg ctacagatga ttgtattaaa ataggtccta tagaaactaa
     8641 aagtagtaat agttcatact ggttgtcatt agcagatata tataaaactg attatttaga
     8701 agaaaaatca aagaatgaaa caactaacac aggatataaa acatctatga caagagttga
     8761 tggtcaatga gaaaattaag agctgataaa ataataaaaa ctattaatcg aggtatagct
     8821 ttaaaccctc aaataataac tatagagcag gaaattaaaa aaatagtgga tggagctatt
     8881 gaggttacaa atgaagtaaa agaactaaca gtcgttatat atcccgaaaa aagcaacgat
     8941 acagtagtaa gtagtgaaac tattggtaca gtttataaaa ataaaaattt tggtatggtt
     9001 gcagataaag aagctgattt aagattgaat acagaaaatg agataacttt taaatgtatt
     9061 gaaggtacta tgaaattaaa ttatgtaaat cctattgtag ttgaggaaaa gatttgtgga
     9121 tatatatgtg gtcttgaaaa gatagattag aggtgggtta gtgtgagtgt gtttactaaa
     9181 gctataaatg aaattgatag aaaaaaggct acaatgccaa ttttgtgtac gaatatagct
     9241 tttatgttag aaggagaagc taagaataat gcaaaatgga ctgataggac aggaaatgca
     9301 agacaaggca taacaggaac aagtttaggt ggaggaaatc aatacattgt taggttaggt
     9361 catggcgttg actatggaac tgttttagag gaaggttcag cacctcatgt tataaaacca
     9421 aaaagtgcaa aagctttgtt ttggaatgga gcttcacatc ctgtaaaaca agtccaacat
     9481 cctggcacta aagggacaca ttctttagag tctacagtca gtaggaatat atctaaaata
     9541 ggtaaattag tagaaggaca ttggagtaaa taatatgaga gcaggaataa gaaaagcctt
     9601 aattgataac ataccaagat taaaagattg ctatgaacca actgttccta ataaaaaaac
     9661 tataaaacct tatattgtag ttgtccaagg tgaagatgct gacaatgaag gtgatgttat
     9721 aggttttaga aggaccataa atatttggtt atatgagaaa aggactacat ttaataaact
     9781 ggatgaactt acaaaagaag tcatagagac tttagatttt aaaactataa cagatgatac
     9841 ttctaatgag gtatttactt gtatttatga gggtgcagtt ggtcaagatg ttatagatga
     9901 ggaatgggaa gccataataa gatgtctaag gtttagtgta atagctttag atgataaaga
     9961 agatataact aatgatagat gggttgaagc tttatctaag tacacaaaaa atttattaga
    10021 aatcgagagt tacaaggata attggaagaa aaattttata gctccatgtg cattatggcg
    10081 aactacaaat attgaaaaca aaaggattaa ctatcatctg attgagatta ctaaaactat
    10141 gaaatgtcat gttgttagca aaaataagga tgaaatagtt aagcttcttg aagaattaga
    10201 gactaagcta ataatagata agagagtaag acttagagaa gataaaaata tgtatttaac
    10261 tcttgtaagt gtagttgaag atagagaatc agacatgttt tctaatggtc aattaactgt
    10321 agtattcaaa atgataggta agataaaaag agaagggcct attatgaata agatttatag
    10381 taatggaaat ttaaaatagg aggtgcaagg attggctgaa gcaattaata aaaagactaa
    10441 tataagtaag caggaagaaa aatattcaaa agaagatttc ttaaaaaata gtgaagcact
    10501 tggctacaag aaagaagtag ttgcaggtgc tttatttaat tgtaagaaag atgaacttac
    10561 aaaagtagag ttcgagaagc ttataaaaga tttcttagaa agagaggtga agtaaaatgg
    10621 caactggcgc atggaatgaa aaagagaaaa aagatatacc tggtttttat aacaggttca
    10681 agactcaagc agaaaagtct acaaatacag gtttaaaggg tagattagca atgcctatta
    10741 gggctaattg gggagatgta ggtaaggtaa taacaataaa aaatgatttg agacaactta
    10801 aaactttatt tggtgatgat atgaactatt cagcatataa gttaggtaaa ttagctttat
    10861 tagggaatgt aaaggaatta cttttatata ggcttgtaga tggaaatcaa aagaagggca
    10921 cattaacact aaaagatact acagaaaata ctgcaaaaga tgtaattaag ctagaaacta
    10981 agtatccaac tagcagaaat tttaatgtaa caataaaatc caatttagtg gattcagata
    11041 aaaaggattt tattttcttt gaaggaacta agcaattatt tagttcaagt gtcaaaggta
    11101 ctatagatga aatagtgcta gaaataaact ctaatttaga taatgagtat gtaattgcaa
    11161 ctaaagtagc tgatagtgat acaacattag caaacttagt aaatgtagca cttgagggtg
    11221 gaaatgatgg ttgtacatct ataacaaatg agtcttatct aaaagcatta gaagaatttg
    11281 agagatatag ttttgattct tttgtacttg atggtgtagc tgaggaagct ttgcaggaaa
    11341 ctacaaaagc ttgggtagct aaaaataaag aattaggaaa aggcatacta ctttttctag
    11401 gtgggaaaaa agaggataat ataaaacaga ttaatgataa atcaaaaagt tttaatgatg
    11461 aaaatatagc taacgttggg agttcagctt attatgaaaa tgtaaaatat acacctagcg
    11521 aagtagctgt ttatatagga gcattagcag taagcaaagg cataacaggt agtatatgta
    11581 atgctaagac tatatttgaa gaagtagaac cacgattaag tcaatcagaa gttaaagagt
    11641 gtttaaagag tggtacttta gttttagatt ttgatgatgg agacgtaata gttgttgatg
    11701 atgtaaatac atttaaaaag tatgtagatg ataagaatga agctatggga tatatctcta
    11761 atattatgtt tattaacact ataaataaag atacttcatt gaaaagaaaa gagtttgtag
    11821 ggaaaatatt taatgatgcg acagggcaga caactgttat atgtgcattg aagaaatatt
    11881 ttgaagaatt aatgagtcaa ggtattatat ctgaatttaa tgttgatata gatacagagc
    11941 ttcaagcaac tgccaaagcg gatgaatttt attggaaatg ggatgctatt aaggttgatg
    12001 tgatgaaaaa aatatatggt actggatacc taggataaag gaggttatag attatgtata
    12061 atgatgatta tatagaagaa gccagttttc tgaatggttc cgatgtagtt atacttattg
    12121 atggtgtaga agagctatac atggaagaaa taaaagctga ttttgagcaa gatgagcaaa
    12181 gtatcaaact gttaggttgt caaaatgaaa tatcaagagt tgggactact aagggttcat
    12241 tctctttaag cggatataaa gtaagctcaa aattcgcaaa gttaggtttt aaatcttttg
    12301 aaataatata taaattaaca aacccacaaa cattgggata tgaaagtatt agattaaaaa
    12361 attgtagatt aaagaaaata ccacttataa attcaaaagc tggtgaagat gtaaagatag
    12421 agctagaagg aagttataga ggatatgatt tattaaacga actttaaaac aaaacagtgc
    12481 agcactcaaa gctatgaaca attaattttg tttgtagctt tttaaaatta aaaatattgg
    12541 aggaataata tgtcagagat atataaaaga gagctagaaa atgaagtaga agataatgat
    12601 ttagatgaag aaatagaaga aacaaatgaa gatagattga agatgaaaga ggatgaaata
    12661 atagcaaagc tattggagga ttcaccagtg cctgagagaa ctgtgttttt agatagatta
    12721 ggaataccaa ttactttgaa agcattaaca gaaaaagaga ttagtaaaat aagaaaagat
    12781 tgcactaaaa ttgtaaaggt ccaagggcga agagaagaaa aattaaatga tgatgagttt
    12841 actctagcat taatagaaaa aggaactgta aaacctaatt tttctaatca aaaattactt
    12901 gatgctatga aagtaacaaa tgctagagaa tttataaaga gaaaattctt agctggagaa
    12961 ttaagcaaaa taagcgacca aatattggaa ttatctggct tttatgatga gataagtgat
    13021 gatgatataa aaaactaata aaaatggggg gacgactgac tgttttaaat aacatctttg
    13081 ttaagcatca tgttccccca gatatttttg ttaagaaaaa tttgatgtct caacgcttaa
    13141 tgaaagtatt tactcaaaat gaaatagagc aagagaataa agctatgaaa aaataaatat
    13201 ctgtagaaag gtaggtgagg gaaatagcta aaaaggaaat gtatcatatt gatgttgtca
    13261 taagtgcaaa aggcgacgga gaaacaaaaa gcaaactaag tactatggaa aaatacatga
    13321 agcagacaga aaaaaggatg cagacactta atagaataaa agttaatcct gctataaaag
    13381 ctacagataa agcttcaagt gttgtaaata gagttaataa caatatgaat aaagcaaaaa
    13441 aaactgttac agcaagaata aaggctacag ataacgcgag tccagtagcc aatagggcta
    13501 gcaataatgt taataaagct aaaaagacag taacagcaag gttgaaagct acagataatg
    13561 cgagttctac agttaataaa gttaataata agataaaaga agttgctaaa cctgtaccac
    13621 ctgtaataat tagaggacaa gatgagtcta gttctataat agataaagtg aaagctaaga
    13681 ttcagaactt aaaagctgat actattataa aaataaaatc tcaagctgat gaagctataa
    13741 acactatttc tcgaactaaa aataaattgc aagaatttgt gagtaagaga tatgaagcag
    13801 cagttaagat tagagatgaa gctagttcag cacttggagg tcttacagga aaaatagatt
    13861 cttttgtaaa tggagctatt agtaaatttg ctagactggc tactaccgca ggagctttaa
    13921 taggtggaat tggtgtaggt tctgctgtaa aaggatttgc aacttttgaa caaagtatga
    13981 agaatacaca agccgtaagt ggagcaacag gaaaagaaat ggaagcttta actgcaaaag
    14041 ctagacaact tgggagagaa actagtttta cagctaaaga tgcaggagac gcaatgtatt
    14101 atatgggtat ggcaggatgg aagtccgagc aaatgataaa agcaattcct gacgttctta
    14161 acttggcagc agcaggagga acagacttag cactaacgag tgacattgtg actgatggac
    14221 taacagcgtt aggaatgact gcaaatgaca caactgaatt tgttgatgtg atggcagcaa
    14281 ctataacaaa ttcaaataca agtgttgagt taatgggtga aacatttaaa tacgtaggct
    14341 ctatgggcgg agcattggga gtatctatga aagatttatc tcttgcgaca ggtctgatgg
    14401 ctagtgcaag tgtcaaggga agcatggcag gtacttcgtt aagaggaggt ctagttagat
    14461 taataaagcc accagaagaa gctgcttctg caatcaaaaa atatggaata gaattaaaga
    14521 aaaataaaaa tggaagctta gatttagcag gaacaatagg aagtcttaga gaaaaattag
    14581 gaggattaga agatgttgaa aaaggtgttg caatatcttc tatttttgga agaacagcca
    14641 tggcaggttg ggcggctgta gtaaatgcta gtgaaagtga ttttaataag ttaactacag
    14701 ctattgcaga atcagaaggt gaagctaaga gaattgctga tatgaaacta gataccctgt
    14761 caggacaatt tgaaatttta aaaagtgcta ttgatgatgt aagaatatcg gtaggaagta
    14821 aactaggtcc tatgactcgt caatttgtag aaaaacttat aaaagatatg cctaaaatag
    14881 gtgatgctat agttggagta gtagaaaagt ttgttaataa tttcgataaa ataaaagcag
    14941 gatttcaagt attattgcct gcaattggtt ccgttatagc ctctgttatg gtacttaaag
    15001 ccacctttgc ttttggagga gctattaaaa gtttaagttt acttgcaaac acttttggta
    15061 cagctaaatt agctgcattc ggattaacag ttggaatagg ggctattgtt atagcttttg
    15121 caggaatgac tgttgctatt tctaataata aaactgctat gatggactta caaacacgtt
    15181 ttggttcgtt tggtgaatat gtaactacaa taatggaaac tgtaggtgga gttataaagc
    15241 ttacgctggg gaatctactt ataatgcttg gagggatagg caaaggaata ggtattcttt
    15301 tatcagataa gagctgggat gaaaaagctt cttcattaaa aaatcttttt ggtaaaacta
    15361 cagcagagat taaaacaaat acaaaagaag ctttgagtga tataaatgga gaaacttcta
    15421 atgcaacagc tctattaaaa aagtctactt caaaagagtt acaaggtgtt actaaagcgt
    15481 tctcaactgc ttttgaccaa tctaaaaatg taacagagaa gaaatctaat gatatagcta
    15541 gagcattaac gaatggatta aaaggtttag atgaccaatc tcttactatg atgcgaggat
    15601 taaatgataa tatggcgata attttatctg gtgtgacagc agatatgaaa cctagtgaca
    15661 aagttaacaa aatcaccaaa aatcttgatg atgctttcaa ggcaggtaaa ttaagtgctc
    15721 aagaatacag aagtagtata caagaaactt taagttttat aagtaaatat agcgcagatt
    15781 cttcaaataa tttaaaacaa ggcatgagtg atgctttcaa tgccttcaag gaagggacaa
    15841 atgtaagtgg attaaaagac ggagtaacag gaatgttgaa ctctttaaaa gctacagggc
    15901 cacaagcttt agaaacctta aaaggtctag gaggtaaagc aagcgaaatt tttaagggag
    15961 tggattttaa ttcttctata gatgcacaga aaactaaagt attacagaat ctaaacaatc
    16021 taggtttgga aggaacacaa gctatagata cattaagaac tattttctca caagcctcta
    16081 ctgcactaga tactacaaat ctaaaacaag gtttaagcga cacatttaat tcattcaaaa
    16141 ttggatttaa tcaagatgga ataaacggag cgataagtaa catgcttgga actataaacc
    16201 aaataggacc acaaatgcaa cagacattag gcaaaatgga tggggaaatg gggcaaatat
    16261 ttgctaatgt agactttagc actccaatag aaacacaagc ttctaaagtt ttagaaaacc
    16321 taaataatct aggaatagaa ggaccaaaag ctttagaaac tgtaagaagt atatttgctc
    16381 aagcttcaag tcaaatacaa ggttcagctt ctcaaactgc tcaacaagca aatcaacagg
    16441 ttgcaaatgc cctaacacaa gaaaacccag ctgtgcaaca agcagggcaa caattaggta
    16501 cagatttgac aaatggtgta gtgaatggag ttcaaacagg ggtgccaact gttcagcaaa
    16561 aagggaatga attaggagta gcaactcaac aaggtgttac agatgcttta aataatgcta
    16621 cacctcaaat taataataac aatcttacaa ctggtataga tacagcattc aaccaagcta
    16681 ctgcaacagt tcagcaagga gcgacgaata tgtacaatgg agcaaagcaa agcttcagtc
    16741 aattagccca agttggaaga gaagcaggaa gtagtttata taacggtgca acaacttctt
    16801 ttaatatgtt agcatcaaat gtaagggttg catgtagtag catgtacaac ggagctagaa
    16861 catcttttac gagtttaagc agttcagcga taagtgctat ttctgctatg tgtagttctg
    16921 tagtaagtca agttagtgct atgtcgagcc aaatcattag ttactggaat agtgtaagag
    16981 caaccgtttc agcaccaatc tctgcatctt ttaatgttaa gactactcaa acaactgtca
    17041 aaagaacagt aaatgaagga ggcgggatac taagtaatat atttggtcga tttgctgatg
    17101 gtggagttgc aagtaaacca agtatttgtg gagaagatgg agctgaaatg gtgattcctc
    17161 tttctaacaa tagaagaaat cgagcgatag gcttgtatga acaaacaggc aaaatgttag
    17221 gtttaagctc aaccccatca caaaaaattg gagaaagtta ttctaatgtt gtaaataatg
    17281 ttagacaatt tcctcttaca aatgttttag atacagataa taaagaatat agagaagcta
    17341 caccaaatag tgttaattct tctaatagca ctataaatct aggcggaata tctataaacg
    17401 ttcaagagag taataacaaa gaagaaatga tacaagaaat attatctcaa gtagaagatg
    17461 gactaagaga agcattacaa gacattggat aatgtcgaat tgttgttaaa aaaaactccc
    17521 tcataaatga tataatattg ttaaaatatg caaatttatg agggggaaat tttatgtggc
    17581 agaaatttaa aagtctaaat actttcttaa aagttattat aggtattata gcaatatgtt
    17641 tcttaccaat cactttagct gttttttcaa ttgaattttt aatgaaagct attgaagcta
    17701 aaaataaagg aaaaatagtc ttaggatgtt ttttagcatt attaacatta agttgtgcta
    17761 ggactgttta tcttacaaaa gatttagatg ataaaaattc tactcaagtc aatcaagttt
    17821 ctagtaataa agataaagaa gatgctgaaa agaaagcgga agaagaaact aaaaaacaaa
    17881 aagagcagaa ggctcaaaca gagaaacaaa aaaaagatga agaagctata aaattagtag
    17941 aacaacaaaa aaagaatgaa gatgagcaaa aatcattgga aaataagaaa aacaatgaac
    18001 caacaaagtt ttttaatgat gtatacttaa aaaataaaaa ttgtgttaac atggcatctt
    18061 atgaaggaat aaaatcaaac atagacatat ttggctataa atataaaatt atagaaccaa
    18121 acgaaactac attaagacag attgatgtat atgacaatca aagcaatgat actgttcaat
    18181 ttcaatttta tgaaaatgat actggagaag aagtcgtaac agttatgcaa tacaataaaa
    18241 aagatgaacc tgacaagtcc gttgctataa gtgatgattt tcatatatat ccaccaaaat
    18301 ttaataatca tattaataat acaaatacag aagttaatag catagaagaa caaataaatt
    18361 ttatgtttaa ttgatatatg tataatactt taaagctctt acaacttgta ggagcttttt
    18421 gttgtaaaaa agaggtgata acttggtaat agacatatac ctaaaaaatg aaaaagaaaa
    18481 aatagatttc cattttccag taactccact tgatagttta tctataaaga aagaaaaaag
    18541 atttgaaact gttgatatag tgaatttagg tgaatttgac attaaaaaag aaggagagaa
    18601 gataagagaa atatcattta aaacatttct accttttcaa tacgatgctt cttattgcag
    18661 atatagcgag ttaaaaaatc ctactgaaat agttgcaatg cttgaaaaat gggtagacca
    18721 aagcgaacct ctaagactta taatgactgg ttttggttac aatggattag ttacaatatc
    18781 tagttttagc aatactcaaa cagcaggaag agaagaagat agagacattg agataacatt
    18841 tagaacttat agagaattga agatagagac attaaaaaaa gaaactaaaa gtaatactaa
    18901 aacagattta aaagataata gacctaacaa taaaaccact actaaaatat atactgttaa
    18961 agcaagtgat acattatata agatagcgaa aaatctttta ggtaaaggtt cgagatggcc
    19021 agaaatttat aatatacctg aaaataaaaa aactattggc aaaaacccta atgtgataaa
    19081 aaaagggcaa aagttggtga taccttctaa atgaaaataa tattaaatgg aaaatatgat
    19141 attgcaaatt ttaatgaagg aataacatta agtgaagcta tagacggagt tgcatataag
    19201 atggatgtat cattagtaga acctaagcaa cttcaagata taggaattaa aaaaggtgat
    19261 aaaatagttc taattgacat tgcgtatgag agtaaaaaag aagaaactat atttaatgga
    19321 gtcgtatggg aaactagaag aagcgaaaag agtaaaaaac tgaccttatc ttgtagagaa
    19381 agaacagtct atatggaaga atcagaagaa caatatcagt ttaaagaaaa tacagctaca
    19441 cagaggattg aatactactg taaacaatgg aatataccat gttacaatct agctaacaca
    19501 ggaaagaaac ttgctaaagt aatacataag actaacatac tagatatgat taaaaaagac
    19561 ttaaaagaaa cagctagtaa gggtggagac ttatttaggg taaggatgga taataagttg
    19621 aaattgttta agcttggcac taatgcaaat gtatataaat tagatagtat attagaagat
    19681 gcaaacttta caagtagttt taatgatgca gtaacaagtg taaaagtgct aggtaagagt
    19741 aaagatgaaa atacaaaagc acctatcatt ggcacatata agaaggactc agataagtat
    19801 ggaacactac aaaagattaa acaagatgaa aagataaaaa atgctaaaga agctaagaaa
    19861 gcagcagaag caatgttcaa tagtggagag gaaacaataa gtgtagattg tgcagtggat
    19921 ataaatagaa tacgtgcagg tgataaggta agtttaaaag gaaaagaata ctatgttata
    19981 gatgtcactc atacattaga ctctatacca aaaatgaagc tcaatatagg aactttagat
    20041 tatataagga ggaaatttta taacaatgac tgatgctaga ttcaatagag ttgctagaat
    20101 attaaaagaa aaaatgaata aaagtgttaa tgatggagtc tttggaatgg gttgttcact
    20161 tgcagaaata acaacaaatg gtctgaaagt aaatggttat aaagatgaaa tacaggacta
    20221 tttagtgtta gagaatttaa cattaaaaga agattatttt actctttcag atgaaatatt
    20281 aggtgcagaa tatagacata aacataaaat agaaactccg aaagagttga aaccattgac
    20341 tataggtgat aatgtattag tagccgttat gggaactgaa tttgtagtaa ttgggagggt
    20401 tgtaaatgcc aaacctattt ccacaaaatg aaacttttga aactgtagaa ttaaaaaata
    20461 ataatgaaaa tgaactggac ctaaaggggt cttttttatt tgattttata aaaggcgaat
    20521 ttgtcaaaaa tgcagatgga acattaaaaa gatgtaacaa ggtagaggca tataaacaat
    20581 ggtgtcaaaa agctatatta acacctagat acaaacggtc agcttattct agtgtatatg
    20641 gaagtgaaat aaaagactta attgctagta atctatctca aagtgctaaa gaacttgaaa
    20701 tatctaggtt aataaaagaa actattttag ttcatccata tacaaaagaa gtgagtaatt
    20761 ttatattcaa ttggcttgaa aatagtaggc ttgttaatta tgaatttgat gtactaacaa
    20821 tagatgatga aaatatagta attgatggca atataaaaag gtaggtgatt atatggaaag
    20881 agaactacct ataccagtat ttttaacgga agatgaagaa gtaatacacg aaaggatgtt
    20941 aagtaacttt caagatgtga gcacactaga aggggacttt atttatgatg caacaagacc
    21001 tacagcagaa gaaatcacac agttaaaaca attaggatta caaaataatt taaagattgc
    21061 atttcctcag acatcttatg gaacttattt agagtggctt ggtgaatgta aaggagtatt
    21121 taaaaatcaa ccaactaaat cggttggcat ggttacattt acaggcgtac aaggagctat
    21181 cattgcaaaa gggactattg ttacaacgat tgctacagat gaaaaacaga gcatagagtt
    21241 tgagcttctt gaaactaaaa ctataggaga aaatgaaaca gtagatatta aagcagaatg
    21301 caggattgca gggattatag gaaatgtatc taaaggcagt atatctgttt tattaggctc
    21361 tattaatggg attaaatctg ttaataataa agaagatttt aaaggtggaa cagatataga
    21421 agacgaagaa cattttagag aaagagtcct tgtgtcagaa caagaggacc gattaagtgg
    21481 agctagttca gattatataa gatgggctaa agaagttgat ggagttggat atgcttatgt
    21541 agtttcagaa tgggctggag caggaacagt aaaagtatta atactagata aaaatagaaa
    21601 agcagcaaca caagaattaa tagataaagt tcaagaatat atatatccat taaatgtatc
    21661 agaaggagaa aatagagacg ggaaagctcc tattggggct ttagtaacag ttgtaacacc
    21721 cgaaacacta cttattaatg taaaagctag ttttatattt agtaacagtt ttagtgaaga
    21781 aacagtatta aacaatctaa aagctaagat agacaaatat ttagataaga ttgatttagg
    21841 tggaacagtc tcatacaatg ctatacaggc aatagtaggt tctatgatgt tgacagacga
    21901 aggtatacag gacttttcta atcttactat aaatgatgta aaagaaaata taaaattgca
    21961 ggaccaagtg gtcggagtag gggaaatagt taacgaggtg gttggatgat agcttctaaa
    22021 aaaggtagag aaatgcttct tacattgtca ccaatatatg agcaatctat tataatgcaa
    22081 agcttatatg aagctatagg aagcgaattt gataatctag atttattaaa taaagaaata
    22141 gaattacaat tatttcctca gagtgcaaca tggggacttg aattttggga aaatagggta
    22201 ggtttatcta ctaatctaga tgaagctata gaagctagga gaagaaaagt tattgctaag
    22261 ctccaaagca aatatattat gacacctaaa agaatgtcta tgatacttca atcttataca
    22321 ggtgcaaata taaaaataaa tgaaaacata tctccatata cttttggtgt tgaattaacc
    22381 agtactcaag gttttcctag agatttagaa gatttatata agagagtaaa tgttataaaa
    22441 ccttctcatc tatctgtaag ttataagtta gtatctttat taaaaagtaa aacttatttt
    22501 gcacaaacaa caattatgag tgaagaaata acagtatatc cttattcaag taaagaagta
    22561 aaagctaata ttaaagctaa gtttgcatta gctcataaca tgagttcaga aatattgaca
    22621 gtatatccaa aaggaggtgg tataaatggc tgatgaacaa ttttacacta tacttacaaa
    22681 tataggtaag gctaagattg ctaatgcagg aatgttaggt aaatcagtag tattagagaa
    22741 gattcaagca ggtgatggag gaggaagcta ttacaatcca acagaagacc aaacagcatt
    22801 aaaaaataaa gtttgggaag gtaatataaa tgtttttgac aaggatgaaa ataatcctaa
    22861 ttggattatt gcaacagcat gtgtacctgg ttcaataggt ggatttacag ttagagaaat
    22921 ggctcttata gataatgaag gcgatatgat tgcaatttgc aaaagtcctg aaacctataa
    22981 gccaaaagtt ggcaatggag ctatgaaaga tttgtattta aaatttatta tagaggtatc
    23041 taatgtagag aaagtgacct tagttgttga cccaactgcg atatttttaa ctaaaaaaga
    23101 tgaagaaaaa atattaacaa atatcaataa gttagacact aaaattgata caactaaaac
    23161 agaattaaca agcaacatag aaactgctaa aacagagtta aacaacaaaa taggagatac
    23221 aacacaactt actacaacag ataaaacaaa tatagttagt gcattaaatg aggtaaaaac
    23281 tagtgtagat agtatagaaa caacagcaga gaaaacaagc tataataatg caacaagtaa
    23341 tcttactgct acaaatgttc aaggggcaat agatgaaatt gttgcaaaaa tagaaaattt
    23401 taacgagatt aatatatcca tacaaaacga tatgttacct atttaagaga ggagagtgaa
    23461 aaatggctat agtttatgaa tttaattata caggtgctga acaaagtgta gtgttgccac
    23521 ctggtaaata taagtttgaa tgttttggtg cttgtggtgg taattattat gattttgtac
    23581 agtgcgcaaa aggtggctat actgctggtt ctttaatatt gaaagaaaat actacattac
    23641 atgtttacgt tgggcaaagt ggttattgta aaggtgttaa tggtattgag acttgtagaa
    23701 gtggttttaa tggtgcgggt ggtataacca cttataaaag tacttctgat ggttattata
    23761 gtcttgcagg tggtggagct actgatatta gacttattgg tggtaactgg gataatttac
    23821 aaagtttgct atctcgcata attgttgcag gtggcggtgg tggtggaagc ggaaattcac
    23881 atgatagtat tggtcatggt ggtggtacaa aaggtaaaga tggtatttct atagcaaata
    23941 aatattttgc aggcggtgga agccaatttc aaggcggttt aacttttaat agtctttata
    24001 atggttcttt tggtgtaagc ggtgctggag atggcattag tggtgttggt ggcggtggtg
    24061 gttggtattg tggtgcaggt agtttttatg ctgaatttgg tggtggtgga agtggatata
    24121 ttctaacaaa agatagttat aagccagcaa attatagtcc atcttctaaa tattatttta
    24181 gcgatataaa tagcgttgta ggtggaaata ctacgaaaca agatggttat gctaaaataa
    24241 cattactcca agcattacct tttttaacta tatcctctta taattccact acagctacat
    24301 ttaaagctga ccacacagac cctacattgc ttacaaaaat agaatatttt atagatgatg
    24361 tactaaaaga aactataaca acagatttaa caacagagaa aataattaac tatacattag
    24421 aagataatgc actacacaca cttaaaatag ttgttacaga cagtgctaat gctacagcag
    24481 aaaaagttgt aagtataagc aaaggaattg cacctcttcc tgctggttct acaacagatg
    24541 aagttacaag taaatggata gaaattaaag atacattcaa aagtggcaaa acaagtatta
    24601 taaatacttt ggcactaaag aatatagaag caagtttaaa taacacatta gtcgagttgt
    24661 cagagaaaat aaaaacaagt tttgatagtt ctgacgctag tgtgcaggat ttgatgaatc
    24721 aattaacaca agctaataat accataacac agttaaatac aaaatataaa gtcgcaagtg
    24781 gcacagttac tccttttaag ggtgatgaca ctaaaattgc ttatccatat ctaactgata
    24841 gagtttttaa acctagcgct tgggttaaaa ttagtaactt agattttaag cctaatattt
    24901 tctttgctga ttttgattac tatgatactg aatataaaaa taattataaa cttttccttt
    24961 tcgcttgttg tggtgttgct acacaaagag gtgttgattt ttcaagtgtt acatcttttg
    25021 ttagaaaaag tagtgatgag tattttcatg ctaatggctg gctctataat aattccgaag
    25081 gagatgttta ttttaataat actggtgttc aaattccagc ttatagcttt gactcaactc
    25141 aaagacatat ttataaatgg tatgctatta aattcattta aaaggaggaa aaaacatgaa
    25201 tgttccaaac agagtaatat atgaccagac aggtagaata atctttgaaa caggcgagtc
    25261 ctgcggggat gtgttgccac acgataaaat aactgaactg cattatatag atgttgaata
    25321 tggaagtata gattatacaa gaaataagat tgtaggcata aatatagaaa caaaagaacc
    25381 tgtcttagaa gaaattccag catatataag taaagcagaa aaggaaaaac aagaattaga
    25441 aaatcaatta cttttaatga caaataaaga actaggtgga ggtattttat aatgaatata
    25501 aataatgttg tagtaagaat attagcagaa agaattttaa acaaaggact gaaccctttg
    25561 aaaaacagac cttttgagtt agatgacgtg actaacatag agtacagaaa agcagtagat
    25621 gattatataa atacaataga acaggaaata aaatagtaac ttttagtaac tcttcttaaa
    25681 gaataggtca ctaaaaaatt ttaattttgt ttattttact tactttcaca tacttataaa
    25741 gaaatcattc taaattctac attaaaagaa acgttcaaat ttaatcatct ttctacttta
    25801 aaagtaacct attctttaaa aatggttact aaaaatagaa aggtgatatt tttatgatag
    25861 aaaaattaaa cgaaaatgct agtttaagtg acttaataac cgcttttgaa aatagtacaa
    25921 aagaattaaa agcaagtaaa gataatttta caaatttgct aggcaatcca tttttagaaa
    25981 gtactaaatt ctcggagttt gagggtaaaa tgcaaaatct tatgagtact tttaaagata
    26041 atttaaaaag taaaggcata aatagtggta atacagagtc tttattgtct ttaattaata
    26101 aagttgcaaa tatatatata cctacgccga tatatacagc aagtggcgag tgtactattg
    26161 ataataaagg tacatgcaaa agatatacaa ttagtacagc tggagacgtt tactacgtta
    26221 gaattaactt aaatttttat ccgaaattta ttgttatgta taactccggt ggagctagta
    26281 aatattttac aaccagctat ttttgtagtt atgttccttt cattgcttca acagtagaaa
    26341 gttatacgac ttacttgctt gataaaagcg atagtaggat acaagttcaa aaaggaacat
    26401 gtattttacc tatagtttac agcaagtatg gtagttctag tttttatttt catgcggtag
    26461 gttaaggagt ggttttatga atgattatat agaagtagga agaaggatat tttttgatga
    26521 agaaggcgaa ataatattct atgaaggaca atcaaaaggg aatgttcccg aaagaaaaaa
    26581 tattaaaaaa atagaatata tagatttgga atacgattat gttgattatg ataagtacaa
    26641 aataataggt ataaatatag agactaaaca gccaatttta gaagaaatac caatctatat
    26701 gagtgaagaa gaaaagagaa tacaagaatt agaaaatcaa ttactaattg cagaaaatga
    26761 aaaagtagga ggattattat aaagaacaca ataatgttgt agtaagaata ttagctaata
    26821 aaaaatagcg aagtagtaaa agaagcagaa ccgtataagg ttcttttttt atagaattag
    26881 ataatttata agttttattt tgataaaaaa acggaggaaa attatggaac aattattaac
    26941 agaattaagt agtttaggag caataggcat attatgcgct ttgctattta aaaataccat
    27001 gcaggagaaa aaagaagata gagatatgta taaaaagaca gtagaaaatt tcatagaact
    27061 atctacacag caacaagaga taaataagaa tatacttgtt gaaatgggag caatgaaaac
    27121 agacgtagaa gaaatcaaag aagatgtaac agacataaaa gacatgttac agaaagaagg
    27181 tctttaaatg aaagtagcaa tagtgccagg gcatacacta acaggaaaag gaacaggagc
    27241 aacagggtat ataaatgaag gaacagaaaa caggatattg atggatttgg ttgtcaaatg
    27301 gcttaaaaag ggtggagcta ctgtatatag tggtaaggta gataagtcta ataactactt
    27361 agcagagcaa tgtcaaatag ccaataagca aaatgtagat gtagctgtac aaatacattt
    27421 taatgccaac aaaacaacct taaatccaat aggtacagaa acaatttaca aaactaataa
    27481 tggcaaagtg tatgcaaata gagtaaatga taaattagcg acagtattta aaaatagagg
    27541 tgcaaaatca gatgtaaggg gcttgtattg gcttagtcat acaaaagcac cagcaatatt
    27601 aattgaaact tgtttcgtag atagtaaagc agatacagat tactatatta aaaataaaaa
    27661 tacagttgca aagctaatag ctgagggtat attaaataag aaaatagata atattgaggt
    27721 gaaacaaatg tataaacata cagtggttta cgagggagaa gttgacaaag ttctagcaca
    27781 agtaattagt tggaactaca aagaaaatga atgtagggta tgtgacataa aagattatgt
    27841 acctggtcag acacaaaatc tgtatatcgt aggtggagga gcatgtgata agatagggtc
    27901 tataactaaa gaaagatata ctatgataaa gggaaacgat agatttgata cactttacaa
    27961 agcattagat tttattaata gataaagtaa atattttata tgaaataatt atgaatgtat
    28021 ggaaaaatat gtttaatata attattaata aggtataaag ttttataaat aaaacgataa
    28081 ggggtggata agatgttaat aaatgataaa agtttttatc caaataatat ttatccagca
    28141 atagactttc tcaagataaa aagacaatta aaatcaatat acaagaatga tttatcagat
    28201 tgcggaagca tatgtataat agaaagaaaa gaatattcta taagcataaa tagcataggt
    28261 gagataaata tatattatga ttcaagatat gaaaataaga tacagaatgt agttgatgag
    28321 attgaacagt tatttaaatc tcaagttaaa aattttagtg tatcaaagtt taagaattga
    28381 tgattagaag gtagcaacag ttagttgtta ccttcttttt tttatgccta cttttctttt
    28441 ttcttccaaa caacctcata tcctatcaaa ctagcaatag tcaaaatttc actatacttc
    28501 atagtctcat tatttatttt ttgccttaaa ttttcaactg tattattttt attattatgt
    28561 tcattcatta aattattcaa ctcatttatg ctgatgtttt cttttacaag aatactttta
    28621 atttcattct tccacgtcat tttccacacc tctcttattt atataataac ataaaaatct
    28681 taatagcgaa agaacaatct atgttaatca aagttatgtc tttgaaaaac aaagaaaaaa
    28741 ctttgaaaaa gtgttgactt agatattcat atagattata atataattaa agaaagagat
    28801 aaacaaaggg ggatataaaa atggaaaaat tcatcagact tgattttgat aaaggtttta
    28861 gaggaaaaga acatagaagt agtgcaacag gagatggaga acattttgaa gcaggaatta
    28921 gctgttataa aataagtaaa gaaaaatgtg ttgatgctat aataaactta tgtgagtact
    28981 ggtttaaaat tgcaggagaa tgtcaattta aagactatga tataaatatt tttgaaggat
    29041 attgtgtagg cgaaggatgt agttatgagg acttagcaac ttgcgagaat catctatatt
    29101 gcatagatgg ctctttattc aatgaagttt atgacttata ctacaaacat gatacatact
    29161 tagatgaaga tggagatatt gaagaattag aagaaaatta caaagatgaa tatataacaa
    29221 cagaagaatt tgaaagcaag ataaaagaaa tgtttattaa atatttataa tacctacagg
    29281 aagtgaaaat atgaatattg gagataagtt cgggaacttt acgatactag atatagagca
    29341 aagaaataaa aggaaatatt atttatgtaa atgttcaaat tgcaataacg aaaaatggat
    29401 aagagcagat agtttgaaaa gaataaaagc gtgtggatgt atgcaaagtg agacacagtt
    29461 taaacaaaat aatttagcag gtaaaaaatt tggcagattg actgctataa aatcaactaa
    29521 taaaaaaaca aaaagtggac attatatttg gatttgtaag tgtgattgtg gaaatgaaat
    29581 tgaaacagca gaaaacaatt taactactaa tagaactaaa tcatgtggat gtttaaaaaa
    29641 agaagctgag ataagaaatg caaagatagc tttaagagta catcaacaaa aaaatattgt
    29701 tgatgataca aatatatcta ttataaaaaa gacagaagca agtataaatt ctaaaacaaa
    29761 aataagagga gtttgttgga ataaagaaaa gggtaaatac catgctcaga tagagtttaa
    29821 gaaagtacat cgcaatttag gatactatga taatatagaa gatgctgaga aggcatatat
    29881 ggaagctaaa gagaagttac ataagaaatt tttaaaagat aatgacaagt taaaagaaat
    29941 agaatatcag atgtaattga atttatttta aaagattata aaattgaata taaaaatcat
    30001 ttataaaatt gaatataaaa atcataaagc tgtattgtta taatacagtt
//
LOCUS       DAGQWR010000040        29671 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.040, whole genome shotgun sequence.
ACCESSION   DAGQWR010000040 DAGQWR010000000
VERSION     DAGQWR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.040"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(168..2138)
                     /gene="ftsH"
                     /locus_tag="MIW95_001521"
     CDS             complement(168..2138)
                     /gene="ftsH"
                     /locus_tag="MIW95_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBY3150852.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2172..3560)
                     /gene="tilS"
                     /locus_tag="MIW95_001522"
     CDS             complement(2172..3560)
                     /gene="tilS"
                     /locus_tag="MIW95_001522"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBY3150853.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3743..4171)
                     /locus_tag="MIW95_001523"
     CDS             complement(3743..4171)
                     /locus_tag="MIW95_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBY3150854.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4188..4994)
                     /locus_tag="MIW95_001524"
     CDS             complement(4188..4994)
                     /locus_tag="MIW95_001524"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBY3150855.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5196..5681)
                     /locus_tag="MIW95_001525"
     CDS             complement(5196..5681)
                     /locus_tag="MIW95_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBY3150856.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5732..6391)
                     /gene="spoIIR"
                     /locus_tag="MIW95_001526"
     CDS             complement(5732..6391)
                     /gene="spoIIR"
                     /locus_tag="MIW95_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBY3150857.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6536..7216)
                     /locus_tag="MIW95_001527"
     CDS             complement(6536..7216)
                     /locus_tag="MIW95_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY3150858.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7200..8090)
                     /locus_tag="MIW95_001528"
     CDS             complement(7200..8090)
                     /locus_tag="MIW95_001528"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBY3150859.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8208..9758)
                     /locus_tag="MIW95_001529"
     CDS             complement(8208..9758)
                     /locus_tag="MIW95_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBY3150860.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9852..10118)
                     /locus_tag="MIW95_001530"
     CDS             complement(9852..10118)
                     /locus_tag="MIW95_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBY3150861.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10266..11126)
                     /gene="yabG"
                     /locus_tag="MIW95_001531"
     CDS             complement(10266..11126)
                     /gene="yabG"
                     /locus_tag="MIW95_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBY3150862.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11210..12148)
                     /locus_tag="MIW95_001532"
     CDS             complement(11210..12148)
                     /locus_tag="MIW95_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBY3150863.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12258..12419)
                     /locus_tag="MIW95_001533"
     CDS             complement(12258..12419)
                     /locus_tag="MIW95_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150864.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12429..12926)
                     /locus_tag="MIW95_001534"
     CDS             complement(12429..12926)
                     /locus_tag="MIW95_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBY3150865.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(13041..13751)
                     /locus_tag="MIW95_001535"
     CDS             complement(13041..13751)
                     /locus_tag="MIW95_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBY3150866.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13927..14457)
                     /locus_tag="MIW95_001536"
     CDS             complement(13927..14457)
                     /locus_tag="MIW95_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150867.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEIVKNVDCN
                     IVQYSNKDERRVKAKANKMAMRLIHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14474..14704)
                     /locus_tag="MIW95_001537"
     CDS             complement(14474..14704)
                     /locus_tag="MIW95_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150868.1"
                     /translation="MSKLVSKVKKDLEIKWLGVDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14719..16113)
                     /locus_tag="MIW95_001538"
     CDS             complement(14719..16113)
                     /locus_tag="MIW95_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBY3150869.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTI
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16239..17492)
                     /locus_tag="MIW95_001539"
     CDS             complement(16239..17492)
                     /locus_tag="MIW95_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3150870.1"
                     /translation="MENKSNFKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALSLS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGVLGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17543..18697)
                     /locus_tag="MIW95_001540"
     CDS             complement(17543..18697)
                     /locus_tag="MIW95_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY3150871.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRKLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIKKLK"
     gene            complement(19256..19825)
                     /locus_tag="MIW95_001541"
     CDS             complement(19256..19825)
                     /locus_tag="MIW95_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY3150872.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            20028..20255
                     /locus_tag="MIW95_001542"
     CDS             20028..20255
                     /locus_tag="MIW95_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBY3150873.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(20474..21217)
                     /locus_tag="MIW95_001543"
     CDS             complement(20474..21217)
                     /locus_tag="MIW95_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBY3150874.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYITDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            21385..22257
                     /locus_tag="MIW95_001544"
     CDS             21385..22257
                     /locus_tag="MIW95_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY3150875.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            22257..22922
                     /locus_tag="MIW95_001545"
     CDS             22257..22922
                     /locus_tag="MIW95_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBY3150876.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            22943..23800
                     /locus_tag="MIW95_001546"
     CDS             22943..23800
                     /locus_tag="MIW95_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY3150877.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGSYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(23956..26502)
                     /locus_tag="MIW95_001547"
     CDS             complement(23956..26502)
                     /locus_tag="MIW95_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY3150878.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(26502..27188)
                     /locus_tag="MIW95_001548"
     CDS             complement(26502..27188)
                     /locus_tag="MIW95_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3150879.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(27271..28260)
                     /locus_tag="MIW95_001549"
     CDS             complement(27271..28260)
                     /locus_tag="MIW95_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3150880.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIVCEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(28247..28933)
                     /locus_tag="MIW95_001550"
     CDS             complement(28247..28933)
                     /locus_tag="MIW95_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3150881.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(29433..>29671)
                     /locus_tag="MIW95_001551"
     CDS             complement(29433..>29671)
                     /locus_tag="MIW95_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150882.1"
                     /translation="IEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         9661 a         5371 c         3411 g        11228 t
ORIGIN      
        1 tataatttta cattttattt aaataatttt aaaataataa aaaactgaaa gtatcaatta
       61 aattaataca tgcaaatata atttgcttat taatttaata ttatactttc agttttagta
      121 tttaatccac atcagataat aatcttaaaa tgaccaaggt cttaaaacta tttatcttta
      181 tcagatttat cttctaaatt tttatttata tcaataacat tgtttctttc ttcttcatct
      241 ttttctaatt ttatagttaa ttgttcttca acttctttag aagaattttc ctctgtaact
      301 gcattttcaa tttcattcag aggtaactct ttattaaatg cttttacaaa ttgttctgca
      361 tctaaagttt catatataag taaagcttgt gctacatact ctaatctatc catattttct
      421 tgtaatatac ttcttgtctt atcatatgca ccatcaacta tacgctttgt ttcttggtct
      481 atctcaaaag caacttcttc agagtaattt ctcttacttg aaaaactatt acctaggaat
      541 acttcatcat cactgtcaaa tgacattgga ccaagtttag aactcattcc atactttgtt
      601 accatacttc ttgcagtagc tgtaactctt tctaagtcat ttgaagcacc tgtagataca
      661 tccttaagta ctagctcttc tgctactcta ccaccaagta aaactacaat attttctttc
      721 atttcatttt ttgtagcata gaacttatct tcaacaggaa gttgcattgt aaaccctcca
      781 gctcttcctc ttggaactat tgtaacttga tgaacaggac ttacttcttc aagtacatgg
      841 gcacaaactg catgccctcc ttcatggtat gctgtaagtc ttctttcttt ctcacttatt
      901 actttcgatt ttttagccac acctgctata acctttgtta tagcttcttc tatagtttcc
      961 atcttaattt tcttttctct ttttctagct gttaatattg cagcctcatt cattaagttc
     1021 tctatatcag caggagtaaa tcctggtgtt cttctagcca agacatccat ttttacatca
     1081 tcacttaatg gtttatttct tgagtgaact ttaaatatag cctctctacc ttttacatct
     1141 ggtgtaccaa caacaacttg tctgtcaaat cttcctggtc taagtaaagc tgggtcaagt
     1201 atatctggtc tatttgtagc tgccatgatt attatacctt gatttacccc aaatccatcc
     1261 atttcaacta gaagttgatt aagagtttgc tctctttcat catgacctcc accaagacct
     1321 gcacctcttt ttctaccaac tgcatcaatt tcatctatga atatgatagc tggagcactt
     1381 ttcttagcct gttcaaataa atctcttact cttgaagcac caaccccaac aaacatctca
     1441 acgaaatcag aaccacttat actgaaaaag ggaactcctg cttctcctgc aactgctctt
     1501 gatagatatg ttttaccagt tccaggaggt cctaccataa gcattccttt tggtattctt
     1561 gcacccaatt caatatattt cttaggattc tttaagaaat ctactacctc ctgcaaatct
     1621 tctttttcct catctagtcc agctacatct ttaaatgtaa ctctagtttt ttcatcatct
     1681 ttatgaacct tagcttttga tttgccaaaa ctcataacct ttccgcctcc accttgagac
     1741 tgattcataa acacgaacca aataatcacc atgaagaata ttaacagtaa tgtaggcaac
     1801 atttcgacaa accaaggtgt agatggctta gcttcaccac caaaagttag tttcccagcc
     1861 ttagcttggt ctaacacctc atctgcaagt ttatttccca tcacttctgt tggaacatat
     1921 gacttaaact ttgtatttgt atcttttata gtaccttcta cagaagtttg atttacaaag
     1981 tataatcttg atatattctc atctgttaat tctctatata cttcagagaa ttttaaatct
     2041 tttatttttt ctgtaggctt acctgagaat tgaactatac ctactataat tataaaaaca
     2101 agtagataaa aacctgcccc ttttagcaac ttattcaata tagaagccct cctttcatac
     2161 attgctttgc cctataattt gttaaaatta atttttaata cttcttttgt gttttcatca
     2221 attttataat tttcactgat tttataatct ccaacacata ttattccttc actatctgtg
     2281 attataggaa ttttacatct ttcttctctt gggattttta aatctatgaa taaatctttc
     2341 acttttttac ttcccatagc aagctttatc ttatcacctt gccttctgct tcttattact
     2401 ataccccctt ttaccttatt aaaatcaaac cctttagatg aattgtctaa tttcatactc
     2461 ttatacctat ctatgctcat tacttgggtc tcaataattt gttttaattc ttttatttta
     2521 ataaacccat tactaggtac attgtaataa aattcaattt cctcattaac aatttcctca
     2581 ttcgttaata ttatactgtc tttttttcta taaacaaata tatttcttgg aagagttaac
     2641 attttattta cttttgaatt atcttctaaa gacattatat cttctatatg tctttggtca
     2701 acaaaattag tatctcctag tatatgtttt atggaatttc taagaactct aactttgata
     2761 gcatcatgta agcaaacaaa atcgtctaaa tttatctcta caaagccttt ttcttttata
     2821 tttgaaactt ctctaaattt agcttctgcc tctttctcaa tataatcgtt atcacttttt
     2881 aaactattgc tcatcctcac tatagattct attacatttg aattaaaatt atccttcata
     2941 tatggtaaaa ggtctaacct tatcttattt ctagtgtata tattttctaa atttgtttta
     3001 tctatcctcg gatttaaatt ataagcttca caatactcct ctatctcatt tctttccacg
     3061 tctaatatgg gtctaattat gcaattatct ctaatataat caattccctt taagcctttt
     3121 aagcctgttc ctctcatgat acgcatcata acagtttcgg cttggtcatt gagattatga
     3181 cctattgcta ttttattggc tttaatctta tccttaattt cataaaacat ttcatatctt
     3241 agcttccttg ctccttcttc taatgataat ccctcatttt cacaatattt tggtacatta
     3301 atagacttta caaagaatat aattcccata tcctcacaaa gtttagatac atataatgca
     3361 tctttttgag cttctatccc tcttatttga tggtttaaat gtgctgcata tatttctata
     3421 ttaaaatctt tttttaatct atttagtaca tgcaataaac aaactgaatc aggtcctcca
     3481 gaaagaccta aaactatttt atcgcctttt tgtattaaat tatgtttatt tatagtactt
     3541 agtaccttat caaatatcat atttatccct cttacactta gttttattca cttttaagta
     3601 tactttctat aaaaaaacgc taaacccttg tatcaatttt aagaaattaa agtttaatat
     3661 aaatataata ataccaaata aattaaataa aaggtagttt tttcactacc tttataaaat
     3721 ttctggaaaa tagccatagt tattatttag tacctatgta tatatctatt ttattcctac
     3781 ctaaaaataa atcttgtacc tttatattat agtctatttt caattttatg tgttcccctt
     3841 cttgtatatt tatatctaat tctcttgtat caatatcaat taataaagta ccttgtgggg
     3901 tcacatactt tgtagtacta ctttctccac atttaaacag cattgttgag ttaatagcac
     3961 caaacttttt gatactaact tgatttttaa gtatctttat agtattagtt atttctatat
     4021 tttcttcttt ttccttgtaa ataacatata ttccatcatt tttataaaaa attttaccaa
     4081 aggcagtcat ctctatggtg tccatatttc ctttttcatc atattgtcta gtatttatac
     4141 ttatttttac actcaacgat ttttcctcca tagattcttc atataaacta gtatttttgt
     4201 atttctactt tttgaatctt atcaattttt ttctttaaga actctcttgc tatatctgaa
     4261 aatttttcag gtatatctga tacataatat tgataagtcc catgaatttc tgtattgttt
     4321 ataatatttt gcacctctaa aatctttttt aaatcctttg cagtctcttt agctggatta
     4381 acaagtttta tatgctctcc aacttcttca cctattgttc tctttaaaat aggataatga
     4441 gtacatccta acactaaaga atctatattt ttttctttca attcttgtaa atatatcttt
     4501 gctgtcaatt tagccacttc tgtatttgcc catccttctt caacaatagg tacaaataaa
     4561 ggacatgctt tattaacaat ttctatactc tcatctattt tagatatttc caagttgtat
     4621 gcttttgaac ttatagttcc ttcagttcct attattccta caattttgtt ttttgtagaa
     4681 cttacagctg ttctagctcc tgcttctata actccaatta tcggtatatc atatttttct
     4741 tgagcttctt ttaagcttct tgcagtagca gtgttacatg ctataactat agcttttaca
     4801 ccttttgttt ttaaaaagtt tatagcttga aaagtatatt taattatagt ttctttggat
     4861 cttgaaccat atggtattct ggcagtatct ccaaagtata ttatatcctc atttggtaat
     4921 atcttcatta tctcttttag aactgttaat ccacctaatc ccgaatcaaa aactcctatt
     4981 ggtttattgt tcatctttat acaacaccta ctttacattt ttgtgtttaa caacaactta
     5041 tttatcgcta aaatttatat ttatattcat aattaatata ttattaccta aatgattatt
     5101 atagtttatt ttacacaaaa aataaaggag ctatcttaaa ttagaaaata atttctacaa
     5161 aaagacaact cctttcacta cttaaaaatt taaatttatt ttgcaaacac atgatttcct
     5221 attctcatat aaggattcaa tctatttatc catttgcaag tggctatttc tggattccaa
     5281 aaatatactg ctttattagt tgggtcagtt ccataaagag cttcctgagc tgctttacgt
     5341 gctgagtctg ttggtgccat ctttattgaa ccatcaagca caactgagaa agcatttttt
     5401 tgatatatga cttcttctat tgtgtctgga aatctagggt ccaatactct atttattact
     5461 acagctgcta cagcaacctg tccctcatag ctttctcctc tagcttctcc tgcaaccaac
     5521 ttagataata acatcaagtc attatcagat aaatttatta cttctttctt tgctatattt
     5581 ttttcttgtt tgtttacttt cttaatattt tctgttggtt catatgcaaa tacctcttta
     5641 ataggcataa atataaaagt caaagctaat agtgtagcca tcagtttact ccacctgtcc
     5701 atgattaaaa ccactcctta cattcttatt tttaaaattt tttctctaaa tccttgacta
     5761 cttcaagtac tttaaactta atttttactc tacttgtttt ttttggtgta tcttgtttta
     5821 ttaacttata ttctttatca gttaaaactt ccttcaactt atcatctgta gatttatcta
     5881 tcactccatt agattcatca acaaaacata gtggagggaa cataacacac caccagtttt
     5941 ttccttcacc tttcccaatg ataatcttta atgctttata ttcaccagct ggaagaacta
     6001 tattagaata ttgtttagta gggaaattgc tatattgtat acctaccttt acactgtaat
     6061 tatatccatt tttaagaata atatttttac ttatttcttc cagactacta tattcctttt
     6121 ttataatagc ttcactttct tttatacttt ttgaattttg aagctttggc tgtaaatatt
     6181 ttattacttc atctctaact tttagtttaa gttcttgatc ttcatctgta ttgctgtttg
     6241 ctataacatg aaatcttatt aatttatctt tataatcttt tgatatattg tctacttttt
     6301 ttacctcacc atttattact attgtcatta ttgatatgac tgatataaga cttaatatta
     6361 atagtcccaa tctaatctta atttttctca ttgaaaattc cccctcaaga taaatttata
     6421 tcatcaatta tgtccaatta aatttaattt attcataata ttttttaata ctaataaaaa
     6481 aagctatctt aaaatgatta caatcatttt aaaatagctt tttaaacctt atattttata
     6541 agtctttact aatcatagcc ttatctatca taaactgctc tgctttttct atatgctctg
     6601 tagcatactt cttggatagc tcactatcac ctttaattat agcatcaagt aagtatttat
     6661 gctcttcaac aatattatgt gatgtatcat aagcagacat ataggtaact ctaaatctat
     6721 atacttgttc ccataatgag tttattagtt ggattaattt tttattatta gttgctttaa
     6781 atatacattc atgaaattct acatcaagtt ttaataacgc atctacatcg gattccaaag
     6841 tgcttttttt aaattcttct gatatatctt taagcttttt aatatcttca tcactaattc
     6901 tttcagcggc aagatatgct gctaagcctt ctaggcttgc tcttacttct aatacatcca
     6961 taatatcctt taatgacata tttgccacat acgcaccttt tctaggcaac ataacgacta
     7021 aaccttctaa ttcaagcttt cttattgcct ctctcactgg agtcctactt actcctaatt
     7081 gttctgctag ttgcacttcc attagccttt gaccaggctt taattttcct tctaagattg
     7141 cttctcttaa attctcaaag actacatctc ttaatggctt gtaattatcc aagttcagtt
     7201 tagttaaatt ctccacaaat ttcaacccct ttttgactgc tattaacaac atacacctgt
     7261 ttatattttt ttaataattc tttttttcca attagagcat cttctttatt tttaaatagt
     7321 ccaaaaactg taggaccact tccactcatc atagaaccta aagcattatt tttcatcatt
     7381 acttgtttta tatcacttat ttctttgtgt ttacctatag taacattttc aagaatattt
     7441 accatgcttt ctgatacagc ttttatatct tcagatttta aacactctat caaatattta
     7501 ttatttggtc tttttttaat attattcaaa tctaatcctt gataaacttc tttagtagaa
     7561 acgaataagt ctggtttgca tatcaatata tttgtatcac atggtaaacc tttgatgttt
     7621 gttagctttt ctccaatacc ttgagctaag gcaggtcttc ctgatataca aaaaggaaca
     7681 tctgcgccca aattaagtcc tatttctttt aattcatctt cagataatct aagttcccat
     7741 aggtgattta aacctactaa tactgcggct gcattagaac ttcctccagc catccctgct
     7801 gctacaggaa tatttttttc tatgaatatt tctacacctt tttttatata aaatttattt
     7861 ttaagtattt tggcagcttt ataaacaata ttatcttcat ctaaaggaat atctaaacta
     7921 gtgcttttta cctttatttc atcttcgtct aattctttta tctttactat atcgtataaa
     7981 tctatcgttt gcattatcat ttcaacaaaa tgataaccgt cttgcctttt tcccaagaca
     8041 tcaatggata aatttatttt tgctctactc tttagtctta taaaatccat tctatcctcc
     8101 ttgtttaaag aataatgcat cactaacaat aatattatac tacaaaaaat aaaataatag
     8161 ggtattataa taccctatta tacttaatta ctttaaattt tttaatatta atctactaat
     8221 acgacttttt tttctaaaat aagacactta ccaggcttaa ttggttcatc aagttttata
     8281 tcattaagtt ctgcaatttc attttctgtt gtattgtatt tttttgctat attccagaaa
     8341 gtatctcctt ctttgcatat atatacgatt atgctaggag ctttagataa atcatataca
     8401 ccttggtctt ctcctttgat aataaaattt tctgctttct tatctaatgc ttcagtaaat
     8461 ctcttaattt tgataatcaa gtctatctgg tccctattca aatcaacttc gactttatca
     8521 atacatgctg tattaaacac agatgctgta tcagttagat tatccatttc tatatcatgt
     8581 tcaaatggaa tttcctcact aattttataa accaatttta aaccttcaac tgggacaaat
     8641 aaaatttcaa cttttatgat tccttgtata atgcttttat ttccttcaat ataactattt
     8701 tctatagaca tggttggaca aacgcttaca atatctttta tttgtatatc atcattatca
     8761 tttcttatgc cttctctaac cataaatgtt tcttcactac gtcttaaagt cttatttaat
     8821 tgaataggtt tgtggtcaaa ttttattatc ttttgaggcg aatatgcatc ttgtaatact
     8881 tctcttgtaa cttcatctgt aacttttacc ttacaacaag ccatacaatc tatttctaaa
     8941 agacctgtgt tgctttcact attttgcttg aaaatatgat taaaatctga catagacaac
     9001 aatacttctt cagtcatacc atcactaact ccagggactt ctacaaattg agtaaactca
     9061 atacctactc tatccaactc tacaagttca ccctcatatg tacaagctaa cggatttatt
     9121 tctaatactc caccaataat aactttattg tcagtcactc tactttcttt tactttaaca
     9181 catggattta aacttattat agattgaatt tcctctgtgt ttattgttat agtatctctt
     9241 atagaacttt ctgctttttc tattccaact atatcttgaa aacaaatttc ctttctgtgc
     9301 ttttgtatgc cttccacttg cgccacatct tttacaatgt ctaacctttg cttttcaaac
     9361 aaacttcccc ttatattcat aagagctcct actttaatct ttctctcatt cattatagta
     9421 caatccatat gctctacttc tgaaaataac atgtattcca tatcttgaac aacattgtct
     9481 ttttctatta cttcgtttat atctactttt ccgtctacat tagaaatagt atttttatca
     9541 tctgcaatat agattacatt ataattaaaa ctacctctgc aaagtatctt cccatctgcc
     9601 atttcaattt ttttaagtga tatgtatcct tctgttttta caatttcata tacatctaat
     9661 tttttgtctg gtacaactgc ctccgcttca atgaatgttt gaaattttcc aaagtctatt
     9721 ctattgtcaa ctttaattac atctttaatt aattccatag tctacctcct taagtattat
     9781 aaaaatatca cactatattt atatttaagg agcaaaaaaa aaattacacc tatttttaaa
     9841 taggtataat tttaacttat ttgtaattta tcttgatctc taaaaacttg caactttaca
     9901 ttagatgtta ataaatctga gtaactataa gatactctag gatatccatt actttcatca
     9961 tctaacttta taacaaacac gcttggataa actttttcta gtataccctc ttttgtaata
    10021 atctgctttc ttcctttatt agctttcaac aatattttct ttcctatatg tctctctaag
    10081 cttactctta tcttatctag agtttgaaca gtagccacaa tatcaccttc tttaacgttt
    10141 atatgtttac ataatatcac atataaacat ttttgtcaaa tgtttaaact aattatttta
    10201 tatatttttt gcaaaaatgt caatactatt tttgaagata tacagtataa ttatgttaat
    10261 ttttgctaat gtaatattgt ttttggcatt gcccatctaa attttcctct tgtttcagaa
    10321 ccaccaattc ccttaacacc tgtaatagtc tgctctataa cctcatcaat aggaactact
    10381 acatcaattc ttgaacaagc aattttctcc actataaaaa cagggtctaa tgcatgtatc
    10441 attattcttc caggcgaact tgcataattt gctcctgcac ttattatttt ttcataattt
    10501 gattggcatg ctcctgcgaa tatgacaaga ttatccaaat ttggttgcca tttacgtgct
    10561 tcttttactg ttttaacaaa atttgaagag tttctatagt tactcatatc ttctatatct
    10621 cctcttttta ttgtcattgc atcatgacct gttattacta gtatatccgg attatatttt
    10681 tctagtagtg ctcttacttc tttatactga ttttgctctg aaacagctac acctactgca
    10741 ggtattccaa gttgagcata tacatccaag caaattttta aatattcttt atctccatct
    10801 atctgtagta cttttccagg cattccatat gtgttaggat ttaattgaag ttttggaact
    10861 gctctagtca ttcttttttg tctttcttta gcttttctta ctgacttata taataaattt
    10921 tctacatttt tatctattaa tatatctttt atatcaggtg cttttactaa ttctaggtca
    10981 tcaatataag catctgctat tactctaaat gcaattccct ttagtatagc tattttctca
    11041 ttattttcat ctacaccaaa agagactatt ttaaatacta tatctttgtt atgtgacttt
    11101 ctagctacaa tgtctcctac cttcatagtt aaacctccaa taaattttta tcttagttta
    11161 tactatgttc gatataaaac tttgtttcct aatgtcaaaa aaacatttct tataatactt
    11221 cttttatcac tcttaaagca tttttatagt atatcttttc tatatcatct tcactaaacc
    11281 cttctttttt tagaggttca tacagtttac ccatctgaga tatatcttca atctcaactt
    11341 ttgaatctat tccatcaaaa tctgacccta gggatacaac atctattcct ccaacattta
    11401 caatatgttt tatgtgtcta accatatctt caagtttact ttcactttta tcacttaaaa
    11461 ataagtggaa gaagtttata cctgtaactc cacctttatt tgctaatact ttaatcatat
    11521 catcagttaa atttctagaa tgattcatca ttgctcttga attagaatgt gtagcaatta
    11581 ttggtttaga agaaatcttt gcaatttcat aaaatccacc atctgaaata tgagaaacat
    11641 caaccaacat tcccaattca ttcatcttat gtaccacttc tcttccaaaa tcagtaagcc
    11701 ctttttctct atattctact ttattatgag gaaagctcaa ttcattcaca tgattccaac
    11761 ttattgtcat cattctaacc cctaagtcgt aaaatttctt taaattttct aatttacctt
    11821 caagtactgc accttcttca atagataaaa gagctgtaag tttaccttct gattggtttt
    11881 tcataatttc ttcatagtta gtagctaaag ctatttcatc actatttttt ttcatttcct
    11941 catgaaattt atttgccata ctcatgcaat aatcaaaagg atgttttact tcttcagtat
    12001 caacaaaaag agcaaatgtt tgagctaatg aatccccctt tttcaatctg tctatatcaa
    12061 cagaatattt attagactta agttcacttg tttctacatt ttccattaat tttgctatag
    12121 tatcacaatg taaatcaata aatttcatat ttataccccc tgtatatttt atatttatat
    12181 ctaaaaatag aaatagttgt ccttatagac aactattgac aactattttt taatattctt
    12241 tttaatatta cttaatctta ttcttctttt aagttaggta ttactttttt aacttcttca
    12301 atttgatgcc atgttttatc tatattattt tcactaatat atttttttaa atcagttaaa
    12361 gatgtaaaat ctttaactga taaattgtca tcatcatctg caaatcttaa aataaacatg
    12421 agactccctt actttccaca acatttctta tatttttttc cgcttccaca tgggcatgaa
    12481 tcatttcttc ctatttttgc ttctttaact attgttttag acttcttata agcctttatt
    12541 atttcttttc tttggtctgc tggtagtata tcttcccaac cttgtaaatt atatagccaa
    12601 tgagcctcaa cagttaccat attgttatat aatttttccc aatctatttt tatagaaact
    12661 tctttatcag cttctacttc ttctaaaact atatcttctt ttaagctttc acttattcca
    12721 tctatgaatc ccatgaaaaa ttcattagta gttccaaatt tttcagccaa ttcacttact
    12781 ttaccttcaa caacatccat tttattattt aatatttcat tgtatattcc agcttcaact
    12841 ttcaaatatt cttcccagaa ttttacttct gcttcttgac tttcatgatt atcacttaaa
    12901 tttctccatt cagtatataa actcatactt tttcctccta agtataccta tatttttata
    12961 gtttttatag tataaaatct tttagttaga ttggaagttt cttctttttg attataccat
    13021 attctattta aagttaactc ttatattata tcataattaa acaattcttt taatactttt
    13081 atagcatact cttggtttat aaaataaggg cttgtcgcaa ttgttattac tttggctttt
    13141 ttaattaagt tttttatctt gtaaaatttg agttcaaatg gtatatagtc catatcactt
    13201 gaaaatatat ccaaatctat atcaagaaca aactctgatt ctacttctaa atcaaatcca
    13261 tacagactat ctattattat taattctgaa aatatgttat attttaaagc aggttttata
    13321 aaacttccca catttaaaac ttcatttgta tacctaaata catcttttag attatttaca
    13381 tctacatcat agttctcagg ctctctagta tccttatgtt ggtctacatg cactaacttg
    13441 catccttttg taaattcacc ctttaacaaa gattttgccc aaaaataaaa tgcatggtta
    13501 tgattgtcaa acacatatat ctttttattt cccaggacat attcgaccat attctccaat
    13561 ccttttgctt ttatttcctt attttcatct acttcattaa ataccacctc attgcctaac
    13621 cttacatcat ttaatgtacc atttataagt tttggaacgt atatgctctt attttctctt
    13681 ttatcatatg aaaatacatt atttcctact ggtttttcta tataaaatcc actatattca
    13741 taattttcca taaaacttta tttcctccat tgttatataa atatataagt tataatttta
    13801 aaaattaaaa tgcacttatt gtaaatcata ttatctatta tagaaaacat cttatataac
    13861 taaatataat atcttctctc aatatgtatt atacaataag tgcaaaatat atttatataa
    13921 aatatactac attcctataa caaaaccatc agcttcaatt ggaaggtaac taactcttct
    13981 attcaactcc acctttccaa aaaatactac ccaatatggt ctaaaaatac tttcaatttt
    14041 aactatttca atctctggga ttcctcccat aaacctatga attaatctca tagccatttt
    14101 atttgccttt gcttttactc ttcgttcgtc cttattacta tactgcacaa tattacaatc
    14161 cacatttttt acaatttcat atgtaggaaa atcttgtact atactaccag aacctgtaga
    14221 accattcact aaaattttta attcatttat atcattagaa tatctcctat tcttgaaaat
    14281 ggatttcgtt ttgtgaattt taagtgtgat aattttaaac tctatataat gaagtctaac
    14341 ttcatgagca tatttgttct ttaatattag cttactaaat aaagaatttt tcccactttt
    14401 agctatctct atagcttctt cttttgtaat tcttataggt aacgctctta tatccataaa
    14461 atcattctct ttcttatttt tttcttgtca tttttaaaac tctatatata taatcaaaaa
    14521 atattacaat tacaactgcc ataactccat atggaattat ttttccaact ataatatcat
    14581 ttctagtcaa tataccataa aatataaatc ctgttattaa aaagtacaat aatactagaa
    14641 aaccgtctac tccaagccat tttatttcta aatctttttt taccttagac actaatttag
    14701 acatagcagt ttctcctttt atagattatt ttgtgtattt aaattttcac ctattctatt
    14761 accaatcatc catgcaataa atgaaaaagg tatagctatc acaagaggct ctaactcaaa
    14821 gctctgaact ggaaatattt taaaataacc aaaagtcact ccacctacaa tcattcctaa
    14881 aataccaccc atagatgttc ttcttccatt tccaagaagc ccacctacta taggtacaaa
    14941 tgccgaaccc atccaaatac ttcctacaaa tatacatgca tcatatacat tttcaaatct
    15001 aaatgctacg ctaagcccca taactgctgc taaacataca cctattctag taaactttac
    15061 taattgtttc tgagataact ctttttttgt aattcttcca tatatatcat ttgctaatgt
    15121 agctccagct gctagataat agctatccaa tgttgatatt atagaaccta aaattcctat
    15181 tataaatatt actcttattc cttgtggtaa tgtatctaaa actaaagtta tatatccagc
    15241 ggctggagcc atctctggat aagtagctct tactataagt cctgttatag ttaacaccat
    15301 gtcaaaacat atccatagtg aaaaacaagt taataaggct cttcttccaa ccttaggaga
    15361 atctgctgct gaaaatcttt gataaaaagt tgggtctgaa taagctccaa aagaatatac
    15421 aattaacatc aatgctttca tagctgacat accttttgta gggtgcataa gctgtggtgc
    15481 atctttgagt gcttctgtga gaccttgaaa tcctcctatt tgttcaaatt gacctggcat
    15541 aaccatagtt aaaccaaata ccatacaaaa aaacaatacc atatctgtaa tggcaactcc
    15601 cataagccca ccaagtactg taagtacaac gactactcct accattatta atcctattaa
    15661 ttctttatct attttccatg ctacgttacc tatagttccc atagcagtta tttctccaag
    15721 ttgtgcacaa tatataaaca tcaaaattgc cccaattata gcaacatgct taccataaaa
    15781 actttccagt aaatcaggaa ctgttatatt ggttcttata tgaatccttg gaccaaccca
    15841 caatgctaaa ggcattctcc caatatgtgc agctattgac caaactgccc atgcagatat
    15901 tccatatata gccgcatact ctacactacc tactgtacca actcctccat accatgatgc
    15961 aaccaaagtt cctactatta aggaccatgg taaacttcga tttcctaggt aataactttc
    16021 catatctttt acatttctag aaaaataata acctattcct aataatataa atgcaaatat
    16081 aactataatc ccattttccc aaaatgttaa catatctaat ctccacattc ttatatttta
    16141 atttttaact ttaatatcta aatatgaaca actctaccca tttccataaa aataggtaga
    16201 gttatataat aattataagt atttaagtaa tcttgttatt aagcaatttt attgtttaaa
    16261 cctttatttt ctcttttatt tattttattt aaagcataag tagcaaatat ccctataact
    16321 gcacatacta cagataaaat aaatatatat gtatatcctg tttgtccata tttatctaac
    16381 caattacctg caatagtgta ataaaacgca tctggagaaa atcctataaa tgaagcaaat
    16441 ccaactactg ctcctgtagt ttctaaagat atattagact ctgaaacaga tgcaaagtat
    16501 atacctctaa atccaaataa aattacactt agtattatca tgtttattac agctatccat
    16561 ataaatcctg tactcttagg aataaccaag taagcagctg tacttactgt cattaatata
    16621 aatcctacaa acattactct tatagatgaa ccaactttat ctgttataac accagcaact
    16681 ggagatgctc ccatttttat aacataagtt cttattattg ataaagcaga aactagagtc
    16741 attggcatta cataaacttc tgataagtat ggacttaaat aagttaaact tgagaatact
    16801 aaataagttg aaaatattat aagaccaatt aaccaagcct ttggcattgt aactactttt
    16861 aacaatgact taatacttaa tccttcatct gatgctccac tatctttttt ctcttccact
    16921 aagaaaaata gaagtatacc acatattata gatgctgcag aatataacca tactacatat
    16981 ttgaatccta ctgttatttc agcaaattta ccaaagaagt aaagacctat aaaagaaatt
    17041 aacgtacctg atacacctga taacccttca taaaaaccaa atattcttcc ttgttctgac
    17101 tcacttcctt gcattctaac aaccttaaca gatgctgaga agaaagttaa tattgttgta
    17161 aatccccata atacaaatat taagagtatc atactatatg atgggaatgt agagaaataa
    17221 aaaccaagta ctcctgaagc aattaaagaa aaagttatca gctttctaac cgagaatcta
    17281 tctgcaaacc atcctcctat aaaataagat accattccta ctaatccata taaactcaat
    17341 aagttcccta tttgtgtatg gctcaatgat aaagcttgtt gcattgggtc ataaaatgat
    17401 gatttcatat atggtaaact atacatagcc gttgttccaa atgccaatat gaatattatc
    17461 aagaatcttt taaaattact tttgttctcc attaattttc tctccttaaa attttattgt
    17521 ttataatctc attcatgtaa ttttatttta atttttttat tgcttgttta aagtcattta
    17581 ttatatcatt tgaagattct aatcctacag ctattcttat tagattttct ccattcattt
    17641 gaactaatgt atgctcttcc cctaagcttg ttgctattgt tgcaagctct aaagagttca
    17701 ttaattttct actagcttgg tctcttgtca atccattgat accatcctta agtttaaatg
    17761 aaactacacc tccacctaaa ccattcattt gagttttaca aagctcatat tgaggatgag
    17821 attttaatgc tggatacttt accaattcca cattctcttc ttcctctaaa aactctgcta
    17881 ctgctaatcc attttcacaa tgctttttca tcctcatatc taacgttttc atacctctta
    17941 aaatcatcca tgcactcatt ggtggtaaag atgctcctgt aaaacaacat aaacttggcc
    18001 atctaactaa atctattaat tcttttgaac ctgctactgc tccccctaaa gtatccccat
    18061 gtccatttat aaacttagta agactatgta ttactaaatc tgcacctaaa tctattggtc
    18121 tttgtacaat tggtgtccca aaagtactat caactatagc taagctttta ttttcatgag
    18181 caatttctgc tattgttttt atatctacta agtctaatga tggatttaaa ggactttcta
    18241 tataaactat tttagtattt ggtctcattg cttcttttac attttgtgga tttactgcat
    18301 ctacaaatgt aacttcaact ccaaatttag ataacaactc agacatgaat aatttagtgt
    18361 gtgaaaatat tccatctgaa ctaactatat ggtcaccact ttttaccaat gctaatatag
    18421 tagatgttat tgcccccatc cctgatgtac tagatatggc actttctgca ttttcaagca
    18481 tagccaactt agcttctaac tctgtaagag taggattaca ctctcttgta tacacatatc
    18541 cccatgtcat acacaactct tcaaaatgct ctactgtctt tgcagcaaat gttgctgttt
    18601 gacatattgg tgtagccaat gcacctgttg ttgggtctgc actatgtccc caatgtatta
    18661 tttttgtctc taaatcttga ttaatattgt tactcatttt gatttcctcc cattttgact
    18721 tatttataca acgttttccc tgccaaattt aatcaattta gcttagtctt aatacatttt
    18781 ttcaagtata cttttttatt tgttataatt tctaaaaata tgacacttat tttgtttttt
    18841 tacagttcta attataaact ctatagtaac tatagagtca atatgttttt gatatttttt
    18901 ttacaatgtt taaaatagtc agtacaaaaa taggtatatc ttagaataac taaagtcatc
    18961 ctaagatata cctattttta tatcacaata taaataatta catataagca ttagtttttt
    19021 tatcataaat agatttttca agatttatca tcttttcata aatatattat agttaaatat
    19081 tttaatatta cctaacatct acaataaaat atactcttaa aataaaatta tataattaaa
    19141 aatactctat aaaagctaaa aagttttcta ttcgctacat ctaaaaataa ttagatagag
    19201 gtataaatgt atttagttta tttatacctc tagtctttta taaattatat ctctactaaa
    19261 ttgatttttt agcacgaata agtgtgctct tggagatacc agtcatcttc acaacctctc
    19321 tatatggctt agaatttaat aaccccaatg catattgtat ctgttgattg ctgtatttct
    19381 ttggcctacc atctttaaag tcctttttct ttttagcaaa ttcttttcct aattgagtac
    19441 gttctattat catatttctc tcaagctctg caactgcaag taatgtagtc aaaaagaatt
    19501 tacccattga agtattttct aaaagaccta tatttaaaat atgaacactt acttctttag
    19561 caaataaacc ttctattagt tgtatccctt ctgatgtact tctagctagt ctatccagct
    19621 tagttgcaac aagtaagtct ccctttttta gagatgatat aattttttca aatttaggcc
    19681 tgtttgtagt agtgcctgta taagtttcct ttattatttt agcatcatta tatttattca
    19741 aaatttcttg ctgttgctga tacaaactgt tgttgtctgt ttgatttttt gtacttacac
    19801 ggcaatatcc atatataata atcatcactt cctaacataa agattttgta cagaagttac
    19861 ccagaagctt gtttttactt atctttatcc attttactaa aacgattttt gggtgtcaat
    19921 actgttaact tttgacacca taataattcc tttataattt gtttgactat gtcaaaaatt
    19981 accaatataa tgaaattaat gagtttatac aaattggagg aaataatatg aatagtctgt
    20041 taaaagaagc aatagatgaa ttaaaaaaca tccattgcgg ggaagttttt ttattaaaag
    20101 aattgttcaa aggctatgaa tggaacaggt tggagatgaa tattagattg aaattaggaa
    20161 aacttttttt gtatgaagtg gtaactaatc ccaatttaaa tgtagaggtc atcaataaaa
    20221 attctgctta tcagcaaatg tataaaaaaa aatagtgact taaatgtata taatagtata
    20281 atcttcttat tgattcaaaa gttaaaactt ttaatacaaa acaatcattc tattttaaca
    20341 tttatcaata tgtctaaatt tatataatta acacatacga atagtattgt ttaaatcaca
    20401 ttatttaaga tagataaatc ttaaccctac tccagcataa atactaaaat agggcttaga
    20461 attatctata tttttatccc ataaaatgct tagctcttcc tcttgattga acatatatat
    20521 ctggggaaat acaatagaag cgtaaattta taaaatttcc aaagtcagaa aacttcagtt
    20581 caaacttagg tataccaaaa ctttcaggtg ctatttcatc aacgccaaaa tagatgataa
    20641 ttccattatc tgtaatataa aatgcttggt cttcaggtat aactaactca acattagaat
    20701 aatacatttc tctattttca tttatttgag atttaatgta atcagtaatt atattaatat
    20761 aatcaactcc atctctaaaa atgtcattaa gtaaaagttc ttttccagtt gtcaaatcta
    20821 tattgtaatt attcaatgta ttgtatttag gcccatagtt attagcaaac cccataaggt
    20881 ttaatatcat actcagtaca taatttttat taaaagtaac tgcgtaactg gtagataccc
    20941 aatagctttg ttttggaagt gaattttgag aagctgcgtt attatattct cgttcttctt
    21001 cctgtaagcc atttttaaaa ttatttacag tcattcttac agcattgttt atgttattta
    21061 aaataactgg gtttacatac gtggattgat ttctaatatt gtatataaca ggatacttta
    21121 catcatatga aaaatactta cttctttcaa aatcttcatt aaattgtaca tataatatat
    21181 tatatgtaca ggtatttgta gattgataat tttccattaa aatcctcccc cttttataaa
    21241 aaaatcagat acaatataat atgcttaaaa cagtaataat gttaatagta aatgtaaaga
    21301 atagaaagta tctaaaacag caaaaaatga ctaattttgt ggtataatac ataggattga
    21361 gagttatttt ataggaggaa aaatatgtta ttattagcag ataaatggaa agattatgaa
    21421 cttatagata tgggaaatgg agaaaaatta gaacgttggg gaagttatgt acttagaaga
    21481 ccagacccac aggttgtatg gccaatggaa tctgaatggg ctttatggaa aaaccctcat
    21541 ggacattatc acagaagcaa taaaggtgga ggtcaatggg aacacaaaaa aagatatcca
    21601 gaacaatgga ctataaatta taaaaactta aagtttcaca ttaaaccaac tggctttaaa
    21661 catactggat tattcccaga gcaagcagct aactgggatt ggatgataga gaaaataaag
    21721 aaagctaaca gacctataaa agtgttgaat ctttttgcat atacaggtgg agctactgta
    21781 gcatgtgcat ctgctggtgc agaagtatgt catgttgatg ctgcaaaagg tatggttaat
    21841 tgggcaaaag aaaatataga attatcaggt cttaaaaatc aaacagtaag atttatagtg
    21901 gatgatgttg taaagtttgt tgaaagagaa ataagaagag gtaaaaaata tgatgcaata
    21961 ataatggacc ctccttctta tggaagaggt cctaaaggag aagtatggca aatagaagaa
    22021 aagttataca gttttgtaga tttatgtatg ggagttttat cagataaccc tttatttttc
    22081 ttaattaatt cttatacaac aggattttct ccaatagttc ttgaaaatgt attgtctaca
    22141 acaataggta aaaagacaaa tacaggagat atatacggtg gggaattagg aataccagct
    22201 tccagagatg gtaagatact tccttgtggt atatttggaa gatgggagaa aaaataatga
    22261 taaaagtcat atatgaagat aaccatttat tagttgtaga aaaaccagtt aacatattgt
    22321 ctcaaggaga taatactaat gataaagata tggtaaatct tttaaaagac tatattaaaa
    22381 aaaagtataa caaacctgga aatgtatata ttggtttaat acatagattg gatagaccag
    22441 taggtggagt tatggtgttt gcaaaaacat caaaagcagc atctagattg tctgagcagg
    22501 taagaagtaa gactttcaaa aaaacttatc tagctgttgt tcatggcaag ataaaatcta
    22561 actcagatat actaaaagat tttttatata aaaataaaaa aactaatatg gtaactgtag
    22621 tgaaaaaaag ccataaagat gccaaagaag ctgaacttaa ctataatgta ttagatttta
    22681 aagaaaatct aagtttagtt caagttaatt taaagactgg tcgttctcat caaataagag
    22741 ttcaatttgc aagcagaaaa catccattat acggagacca aagatatggc aaagaaataa
    22801 ataagattgg acaacaaata tctctttggt ccaataagat agaaatttgc catcctacta
    22861 caaaagaaaa aatggaattt acatgtcatc cacctgacaa atatccatgg aatatatttt
    22921 aaatctcaaa tgaggtgatt atatgagtaa aaaattttca ataggagaaa tgtctaaact
    22981 tcataaaata agcatacaga cactaaggta ctatgaccaa atagatttat ttaaacctgt
    23041 tcatgttgac caagaaagca attacagata ttactcaatt gaccaatttt cacaattaga
    23101 tattataaaa tatttaaaat atttaggtat gcctttaaaa gaaattaagt caaagcttga
    23161 tgaaaataat gaagaaattc taaaattact aacaaataaa attaatatag tggatgaaaa
    23221 aattaaagaa ttagagctta taaagaaggt actaagcaca aagaaagaaa ctatggaaaa
    23281 aggtatgaaa gtagatgaag ttggtaaaat agtgagaaaa catattccac gaagaagtat
    23341 cttatctata gagtatgaag aaggattaag tagtgaagaa actatggaac tttccagaag
    23401 acaaatagca aatatattag aagataatat atctgtgttc tatggtggag taagtggact
    23461 tgtatctata gataaatttt taaaagaaga taaagttatt tatgacaata gttttgttac
    23521 tgttgaaaga gacttatttg atataaaatc acaaaaacat ataacagaaa tttcatctgg
    23581 tgattttatt tgtatgacat ataaagggtc ttacaaagaa aattataccg cttttaagaa
    23641 actggttgat tatgttgaat ataaaagaat acctattgaa aattatatat atgaaatacc
    23701 tataatagac cctctgtcta caaacaacga agaggaactt ctaacagaaa ttcaaataca
    23761 agttaaaaag agagaaattg acttaagatg tctagtgtag taatctagtt tacttacaca
    23821 tcttaatata gttgtctatt atcaacatat gatttttcta gatagtgatt cctaacagtg
    23881 agattttcaa aaaataggag gaaatatggc taatgctaat tccctcctat ttttaattaa
    23941 tccatatcat tttaattaag ccatgtcatc acgaagtgca tcaataatat tttctttttt
    24001 tatcttatta atagtataca acattgtaat aaatactata aataaaacac taaatatact
    24061 aattccaata ctaaaccatg gaatcacaaa atcaatatca tctgtaacca ttactttatg
    24121 aattagccat gaagaaatta ctgctatagg aagtccaaag aacaatgctc tcatgccata
    24181 aaaagcacac tcaaaattca tcatcttttg gaaatcatgc tcagacattc ctacagagcg
    24241 aagcatggca agttctctac gacgtaactt aatattagtt gaaattgtat taaacacatt
    24301 ggcaatcgaa atcagagaaa tcataattat gaaagtatag gcaaaaacat tggcaataaa
    24361 aatgtaatta cggttctcat ccatcattcc atacatattg tacagattgt actcattagt
    24421 aactccctca ctctcaataa tttgctccat ttctgcaact gactgagtag gatttttcga
    24481 ttgaaagctt aaactttcaa ttcctgtaga aacaccagga atctcaaact tatctttggc
    24541 tgaataagga gccaatactc tgaagcttgc tttcttcaca tctgaacttc tgattcctgg
    24601 aggagaatca ggtggcacta ctttgacaaa tgaaatattc acattttgtc tctcaggtat
    24661 ctccaacttg ccattagttt caggagtgag actaacattc atagaagaac tgttgaaaat
    24721 atcaataata ttattatcct ttgttaccat agaaacagca atcagttttg cattttgtcc
    24781 agtatattct tctgctggta atccaagact tttaataata ttcagataag ttctatcatc
    24841 aagaaattgc atatctactg gtaattcaag catttcactc atagaatgtg aaccctcata
    24901 gttcaaataa tcgtctgtaa gctcacttac tttggcaaca catgaatagg tcataagttt
    24961 ctgatacaaa ccttcgtaaa caccatttgc agtttttagc ttatcgtaaa gcttcaacat
    25021 ttcgttgtct ttcatacctt gtgtagaaaa aataatatcc tgatcagtaa cctctgacct
    25081 ctgatttgac atctgtttta ggtctgctac taaagcactt gtagatataa ataaaactat
    25141 gcttaaaaca agcgacaata caatacttcg ataacgtttc ttgtttcttt taaagttttt
    25201 catagcaaga actccctcaa gaccataaac tcgctgtgtt agttttgatg tcttcacaga
    25261 tttagattca atttttacct catttgtctg acgaatacac tccatcactg gtgtgttggc
    25321 agcctttctt gctggtatgt aagcagaaat taagattgta accatactta ccactgctgc
    25381 aacgatgatt gcagggatag acaatgttaa tgtcaacgga acgtttacat agagaatatt
    25441 tgcaaagttt ttagctatga cagaaatcac aagtccaata ctgactatac cgattgcaac
    25501 accaattggt ataccaatcg caccaatgca aattccttca aatagcaccg aatttcgtaa
    25561 ctgttttgct gtagctccaa cagatgaaag tattccaaat tgatgtgttc gctcgttcaa
    25621 tgacatgtta aatgaattat atattagaaa aattgagcct agcatgatta tggcaagcac
    25681 aatgccacca accgagtaca aaaacatatt gaacaaattg ttatcagaaa cacctatgaa
    25741 gcgcaacaca ttgtcattga aaatggaagc atggtcttta gtgctacttg tgtaagagcg
    25801 aatctgatgt ggatttttca atgtgacaaa aaggctaaga ctgtcagtct tgacttgttt
    25861 atcttctttg gttatcaagg tatatcctgg tgcagaagat tcttcaaata cgggctttct
    25921 acagataccc acaactgtat aagttttctt atcctgtggc acaaaaactt cactcccagc
    25981 agtgtagggg tcactctgac caagcttttc attttcactc attcgtttcc ctacatccaa
    26041 agaaatagta tcacctactg caaatttgac accaccatct gtcgcaactt tacttgaaac
    26101 aagaatctct ctactatttt caggtaatct tccagaaatt aaagttatag gcaaagtatc
    26161 aaaagcttct ttgctaaatc cagcaataaa aagatatggt ttatctggat ttctgttacc
    26221 ttcaagtgtt gcatagccaa tattttcaaa tgttgcagta tttgcaactt catcattgac
    26281 tctttgttcc tgtacaaaag aagaatccac atccaaaaac tggacatgcc aatcaccata
    26341 ttttataata gctccatttg tcatatagtt catcaaagaa actccaaagg tagtaactgc
    26401 tgtaatcata gcagctgaca gaacaactcc tataattgtt acaagagttc gagtgcgatt
    26461 ctttttcaat ccttgcagag caactttgtt aaaaatgttc attaacgatt caccttcctc
    26521 tcatcacgca ccacttttcc atctgaaata cagataattc tatctgcctg taaagcaatg
    26581 ttttcatcat gtgtgacaat aatgagagtt tggtgatatt tactatggct ctctttcagt
    26641 aactggataa tctcttgtcc atttttactg tctaagctac ctgtaggctc atcagcaagc
    26701 ataactgctg gtgcattcat caatgcacga ccaatggcaa cacgttgctg ttggccccca
    26761 gaaagttgat ttggtaaatg tgtttttcgt tcttttaaac caagtaattc taacaagtca
    26821 tttaaccgtt cttgattgac cttacgctta tccataagta taggcaatgt gatattttcc
    26881 actacattta atgtgggtat cagattgtga aactgataaa tcaatccaac ttgtcgccta
    26941 cgaaaaatag caagtttttc attgctttgg ccatatacat cttgcccctc cagatatatc
    27001 tttccacttg ttggtacgtc tactcctgca atggaatgta gtagtgtaga tttaccagaa
    27061 ccagaagaac caatgattgc agtaaactcc cctttttcaa tagtaagtga gacatggtca
    27121 agtgcagtaa ccttattttc attcttacca taaaccttac ataaatcttc aatctttaaa
    27181 aacgccatta tataagtctc ctttcatatt ttataaatat atcatagaac tttcaactta
    27241 catttaagtg acatttaaat gagagattcg tcactttgga aaacgaataa caaatattgc
    27301 accaccttgt gggtgatttt gtgcagtaat tgttcctcct tgtctcataa taatcgtctt
    27361 acaaagtgcc aatcctattc cataaccagc tgtgcttgaa tttttcccac gataaaatct
    27421 gttaaataga catggtaagt cttctttttt aaagcctgct ccgttgtcat gaatagtaag
    27481 ttgagtaaac aaaaagttgt cctcacaaac gatatcaatt tttccattat cacccacact
    27541 ctccatgcag tttttaaata tattttgaat tgcttctgaa agccaattta aatcacccaa
    27601 aatccttact ccttttggta tatcaatatg taaagtgata ttgtgcagtt ccatgggaat
    27661 caaaagttga tgaagtgcag aactaatcaa attattcaca tcaatttgtt ctttttgaaa
    27721 gactacaata cctgcatcta aacgtgataa ttttaaaagg gaagtaatca gccagtccat
    27781 tcgtataagt aattcctctg tttctcgtat caactctttt ctctcatctt catcagaagt
    27841 attctctaac agtgacaaaa taagagttac tgatgtaaga ggagtgcgaa gctggtgggc
    27901 tatgtcagct agtgagtcag caaggtgttc tttttctttt ttcagtgcgt aattttgctc
    27961 tctaatacgt aacgtcattt tagttatttc actttgtaga atagaaagct caccctcatc
    28021 tgattcagca atatacagat ggttagcatt atgaagcaca agattaattt gttctgaaat
    28081 ctgtgcaata cttttgtatc gatttttagt aaacacaaaa aatgctattc caaaggtagt
    28141 ggcagaaaca atagaaagta ttccagtcat aatactgatt gcaaatccca gggctacagt
    28201 gagaatagta attaataaga ataaaatagc aaattttcga aactctctat ttcgaaacat
    28261 aacttccccc caatctatac cctgtcccac gaacagtcaa aattatttgt ggacttgaag
    28321 ggtcatcttc tatcttctct cgcaagcgtt tgatgtacac agtcaatgtg ttgtcattaa
    28381 caaactcacc tgccgcatcc cacaattcat caagtaatct taccctcgta ataatacttt
    28441 taggattgct aataaacacc aataacaaac ggtattctaa agctgaaaga aaaacctcat
    28501 tgccattctt tttcacaaca ccggttgccg tatctacata aagcccacaa atttcaaaag
    28561 ttgacccaaa acgcccactt tttcgtagtg ctgttctaat tcgcgcaatc agttcacgtg
    28621 gacgaaaagg tttagtaata tagtcatctg cacccatatt cagtccagta acaacacttg
    28681 attcatcacc agaagccgtc aaaaagataa caggaatgtc ttgcttttct ttgatttctg
    28741 tgcaaatcgt aaacccattt ccatcaggta aagaaatatc aataagtgcc aaatcaaatt
    28801 tattcttagc aatctctaca agtgcttcac ttcttgtgga tacatgggtt actataaatc
    28861 cttctgaatt aagcaaaaga ataagatttt tggcaattgc tttatcatcc tcaactaaaa
    28921 atatccgttt catttacttt catcctcctt tattttatta gttttatata ttccagcaga
    28981 gcaatagtac actactattt tacttatcat tacacaattt tcagtagttt cttttgttct
    29041 tatataatcc atgcataata cctccattga tttattatat ttcttataac ataaatacac
    29101 aagataagac aaaaaacata atgtaagtgc aagtttttta actcactaac ttataaacat
    29161 acactcctac taacactact ttcttgatga cacatcttac aagcttatat tcatacatca
    29221 atgaaaaatt aaatttatcc atttattaaa ataaaaggca atagcttcta tagctaatgc
    29281 cctttatctt aaaatgattt aaatactata caatttttat aaatggctat aaataaactc
    29341 gtatagaata taaaatttat ctacagtctt ttgttataca aaatttataa cctcttatag
    29401 aacaaattta ccactttgta aacttatcac ttttactcag ctatattata aacttttaat
    29461 ttatctttgt ttaaatgttt cttcttaact tctactaaag cttttagcgt atccaaagat
    29521 gtcattattt cagtagaaaa ttcaatggct gttcttctca tcttaaatcc atctcttgta
    29581 gagtcatttc ctttagttgg tgtatttatt actatatcaa cttgtttatt tcttatagca
    29641 tctaatatgt ttggtctagg ctcttcaatt c
//
LOCUS       DAGQWR010000041        29049 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.041, whole genome shotgun sequence.
ACCESSION   DAGQWR010000041 DAGQWR010000000
VERSION     DAGQWR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29049
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.041"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            384..1088
                     /locus_tag="MIW95_001552"
     CDS             384..1088
                     /locus_tag="MIW95_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3150883.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            1090..2577
                     /locus_tag="MIW95_001553"
     CDS             1090..2577
                     /locus_tag="MIW95_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3150884.1"
                     /translation="MNNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVFSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            2782..4395
                     /locus_tag="MIW95_001554"
     CDS             2782..4395
                     /locus_tag="MIW95_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY3150885.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYEILEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNIHSQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQASFGNKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDSSVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(5085..5915)
                     /locus_tag="MIW95_001555"
     CDS             complement(5085..5915)
                     /locus_tag="MIW95_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY3150886.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPITYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKI
                     "
     gene            5988..7715
                     /locus_tag="MIW95_001556"
     CDS             5988..7715
                     /locus_tag="MIW95_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3150887.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLIMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFVKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNREHFEKKRF
                     DRTAIDYSETAISINKVFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALI
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGRKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDVKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEIKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            7752..9614
                     /locus_tag="MIW95_001557"
     CDS             7752..9614
                     /locus_tag="MIW95_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3150888.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKVKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRRAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFSSEYDEEVV"
     gene            9947..10636
                     /locus_tag="MIW95_001558"
     CDS             9947..10636
                     /locus_tag="MIW95_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBY3150889.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNNCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSIDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            10741..11427
                     /locus_tag="MIW95_001559"
     CDS             10741..11427
                     /locus_tag="MIW95_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBY3150890.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKYGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            11444..13351
                     /locus_tag="MIW95_001560"
     CDS             11444..13351
                     /locus_tag="MIW95_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBY3150891.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLTSVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETHKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDKKEVNAELTSSVVKT
                     NTDNETENKTTNDSESKNNTDKDKSGNDNNQKLEGPRGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            13804..14493
                     /locus_tag="MIW95_001561"
     CDS             13804..14493
                     /locus_tag="MIW95_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150892.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            14929..15468
                     /locus_tag="MIW95_001562"
     CDS             14929..15468
                     /locus_tag="MIW95_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3150893.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            15487..16530
                     /locus_tag="MIW95_001563"
     CDS             15487..16530
                     /locus_tag="MIW95_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3150894.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            16532..17368
                     /locus_tag="MIW95_001564"
     CDS             16532..17368
                     /locus_tag="MIW95_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3150895.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            17473..18147
                     /locus_tag="MIW95_001565"
     CDS             17473..18147
                     /locus_tag="MIW95_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3150896.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            18147..19199
                     /locus_tag="MIW95_001566"
     CDS             18147..19199
                     /locus_tag="MIW95_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY3150897.1"
                     /translation="MIKFKKTISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            19643..20491
                     /locus_tag="MIW95_001567"
     CDS             19643..20491
                     /locus_tag="MIW95_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY3150898.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            20481..22145
                     /locus_tag="MIW95_001568"
     CDS             20481..22145
                     /locus_tag="MIW95_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY3150899.1"
                     /translation="MMFKIFNKSLLKLVIVLSIVLTISNFSLFSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINENYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKVTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKYISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            22194..23450
                     /locus_tag="MIW95_001569"
     CDS             22194..23450
                     /locus_tag="MIW95_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY3150900.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYKVSKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIEGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTTVIISDILCVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            23473..25554
                     /locus_tag="MIW95_001570"
     CDS             23473..25554
                     /locus_tag="MIW95_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBY3150901.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASSFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGTNINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKPKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            25591..26676
                     /locus_tag="MIW95_001571"
     CDS             25591..26676
                     /locus_tag="MIW95_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBY3150902.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDGSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWVENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDSSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            26730..27854
                     /gene="wecB"
                     /locus_tag="MIW95_001572"
     CDS             26730..27854
                     /gene="wecB"
                     /locus_tag="MIW95_001572"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBY3150903.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKALVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDSYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            27932..28906
                     /locus_tag="MIW95_001573"
     CDS             27932..28906
                     /locus_tag="MIW95_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBY3150904.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSVTIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDIIRSYNLQLIDNKVETK"
BASE COUNT        11568 a         2860 c         4955 g         9666 t
ORIGIN      
        1 attgtttgaa tattcaataa aaagtttaaa aaatatcaat tttgatataa actgaataaa
       61 tacagttgaa tattaaatta ttattttatc aaaaatatgc aaagtactac accttcaaat
      121 atcacctaaa gtaattaaaa ggcaattaaa gtgaagtagt tcaagtattt ctaaaatgag
      181 tttaataaat tctataataa tttatttttt gccttaaaat tgccttaaaa tccaaacaat
      241 tttaaaacta atgtactata aaaagctggt ataatttaaa tatagtaaaa aataagattt
      301 atcttatcta ttgggattta attatttaat caatatattt ataccattta atttaaataa
      361 aaagtctatt aaggagtgag cttatgagta agttaattta tatagcagat gatgaagata
      421 atataagaaa tttggtaaag acattcttaa aaaatgaagg tcatgatgtt atggatttta
      481 aaacgggtga tgaactttta gaacaattta atataaagga gtgtgactta gtaatactag
      541 atataatgat gcctggctca agtggttttg aagtatgtac aaaacttcgt gagaaaagta
      601 cagtcccaat aatcatgctt actgcaagag atacagatat agattatatt acagggataa
      661 ccttaggtag tgatgattat tttacaaaac catttagccc aatgtctttg gttatgagag
      721 taaaatctat atttagaagg atagagtttg aaaagaaaca aaattatgat aaatatagta
      781 actcgataga tatggaatta aaatttggtg atgtaataat aaataaaaag aataaaatcg
      841 ttacaagtaa aaatgtaaat atagacctta caccaaatga atataatcta ttgacttatt
      901 tgtttgaaaa tatagataga gcagtttcca gagatgaatt attaaacaaa atatggggat
      961 acgatataga agttgagaca agggctgctg atgatacagt gaagagatta cgtaaaaaaa
     1021 ttttagatac aaatatttta atagaaactg tatgggggtt tggatttagg ttgaaggaga
     1081 aaagttaaga tgaataatgg aaatgacaag aatagaattg gaaagattaa atccaaattt
     1141 aaattaaagt tctcaaagaa aaatataagc ataaaaacta ggattttgtt gagcatactt
     1201 tgtttgataa ttatagtatt tatgataata ttagtatctt ttaatgtatt tcttgataca
     1261 tatataaaaa caactgcgaa tgaagaattg acgaagtcta cagaaatagt agaacatatg
     1321 gatagtaatt ttagacctat taagccacca caagatgatg gaaagcttcc tcccaaagta
     1381 ttatctaatt ttatgagaaa tgtacaagat aaagttagga tggcagaaac acaatcagat
     1441 gctgatgcta tggtagttga ttctaagtat aacctgatat ttccaacaag agaggatgat
     1501 ttcttaaaaa atgttgatga gatggagctg attagggagt gtattcaaaa tgaaaaatta
     1561 gatttaaatt cagatgaaaa tacacggata tctaccagaa ataaggatta ttatatttca
     1621 actgtaaaaa ttactagtat atatggtgaa caagacaaat atcttatact ttttatagac
     1681 atatcaaaaa ctctaaatct agcacaaaag ataaatattg tattaatatc tgtaatgtgt
     1741 tttgctggaa tattagcaat atttacagct gtgattttat ctgagaaaat tgctaaaccg
     1801 ataaaagagt tgtgtgagtt tgcaaaaaaa atgggtcaag gagatttcaa aagaacctac
     1861 tttgactttt cagataaaga attagtagag ctctcaactg ttatgaataa gagtgctgag
     1921 tatctggata agtatgataa tgaacaaaaa atattttttc aaaatgcatc tcatgaatta
     1981 aggacaccac tcatgtctat taaaggttat gcagaagcta taaaatataa tgttatagat
     2041 agtaaacatg caagtgatat aattcttgaa gaaagtgata ggttaagtga tatggttgaa
     2101 gatttattat acatttctaa aatagataat ataactaaag attttgaact tgtagaatgt
     2161 gatttaagag aagtattttc aaattgtgga gctaggcaaa atgtaagagc tataaataag
     2221 gggataaaat ttatttatga atttgatgaa gaaatggttt tatttgaatg tgatgaaaaa
     2281 aacatttcca aagcatttat gaatcttata gaaaatgctt tgagatacgc taagagtgaa
     2341 ataaaaatag tgtgtaagta taatcaaaaa aatatagttg tgataataga agatgatgga
     2401 attggaatca agaaagaaga tttacctcat gtttttgaaa gattctataa gggagtaggg
     2461 ggaaatcatg gaattggatt atcaatagtt aaatctattg taaataagca tggtgggcgt
     2521 atatacgtag aaaatggcaa aaaaggagct aagtttacta tattatttaa attgtaaaaa
     2581 aattagagaa aatataaaga tgtttgaagt tgttttttat atatatctgt tgtatactgt
     2641 tgaaaaaaga acaatattaa gctgacatat attacaataa ttgacattta aagtaaatgc
     2701 ttaaatatag atacgaaagg aaaagtaaaa tctgaagtgt atttaggttt aagtaagctt
     2761 cttattttat aaaaaatatg tatgaaagaa cagctgattt ttaatattac aaatttagta
     2821 acggttgtat ttgaagcttt tgtcatccat atgtttttat cagacttttt aggtcagaaa
     2881 gaagggtata taaaagttgt aagatatgct aaactgggtt ttataatatg tttaggattc
     2941 tgtaatttaa taactttaaa ccctaaaatc acaatgcctt taatttttat ccttattttt
     3001 tcaacatcat ttttatataa aggaaattta aagacacgat tgtttactac tgttttatta
     3061 agtatattct ttatactgtc agaaattgta gttacaagta tttttgtttt atttgtaaaa
     3121 gaaggctttg aaataatgct tgaaaataat agtataagag tattggcgac aattttatct
     3181 aaaattgtct ttttgttgac ttgtaaaatt atttgtctat ttaaaaaaga tgtacattta
     3241 gatatgccca taaagtattg gctcccttta tttttaatac ctatatttag tttatttctg
     3301 tctgtttcta tctttgatgt aagtaaattt tttagtcttg aaagtttaaa atttctaagt
     3361 ttaatatcta gtgttggcat tttatatatt aattttattg tattttattt atttaaattt
     3421 attatagata aaactaaact atctatgaaa tatgaaatac ttgaaaaaga aataattcat
     3481 aaggaagaat taagacttag taatgagtgt tataaaatta ttgttgaaca gactgattct
     3541 gtcgttttcg aatggaatat caaagaaaat aaatcatttg tatcgcaggc ttggactgaa
     3601 aaatttggat ataacaatgc atgtaagaat atttttaaag aaattaaaga taaagactta
     3661 gttcattctg aagataaagc tatatttgaa ggttttcttg aaagtattaa aaaaaagaat
     3721 atacacagtc aagcagtata tcgtttaaaa aaatctaatg gtgaatatat ttggtgtaga
     3781 acatcaatta caagcattta taatgatgaa aatgaacttt taagagttgt aggagtaatt
     3841 gtagacgtag atagtgatat aaaaaaatat gaagagttaa gaactagagc agaaagtgac
     3901 tcattgacca atatatacaa taaaggtaca tttgaaaaat tagtggaaga aactatagtt
     3961 atgaatacag gtgataaaaa agatgcatta ttcattattg atttagatga ttttaaagaa
     4021 ataaatgata attttggtca tccatttggt gattttgtac taaaaacatt tgctgataag
     4081 atacaagcta gttttggtaa taaagactta gtcggaagaa ttggtggaga tgaatttgtt
     4141 gtatatatgc aagattatgt tacagaagta aatttgcata aaaaagctaa agaattaaat
     4201 agagtacttt cggataatta cactgatttg agttttagtt ttgattcttc tgtcagtata
     4261 ggtattgcaa ggtatcctca agatggaact tcattttttg aattgtttaa aaatgcagat
     4321 agagcacttt atagcataaa agcatcaggt aaaaattcat attgtttatt tgaagaagaa
     4381 ctatatgtac agtgaatttg aatttagatt ttatgtgaga gtaagattta tatatacttg
     4441 tggctgtctg atttataaaa tatgttactt tgtatatttt tatatgtttc agtaaggtta
     4501 attaacataa gcaaaggtgt aagtaaattt atattagttt aagtttactt acacctttat
     4561 aaaaataaat tttgtaaatt taaacagttg gttttataag gatttttatt tattttaata
     4621 tcttagatat tcctttttgt attccttaga gaaaataaat aatgaaagga gtacagaaaa
     4681 aattaaacat cctaatatac cacttgaatt aagtcttaaa gcattaaagt ttaaaaaagc
     4741 attatttaaa tcggtatcca aaagaaaagg atgctttgat ttgctatata tggaaaaatt
     4801 aaaaatatat tcataatcaa atttataaaa ttaatatctt tccatcctaa cattacattt
     4861 acttagatta attattatta taaaaataaa aatacaaata atagattcat ttacattctt
     4921 aaatgattaa agctctttac cctttaaaca tgcataacat atcgttataa ttccagtaaa
     4981 taagactaaa atgcccattc ctaaaattgc agtttcatca tctattactc caaatgcttt
     5041 taaagtaact tatttcgctt tgtataataa taatttgtct ctttttatat tttaacttct
     5101 gctgagattt gagttagata ttcgttagaa tctcgcacac ttatatcata tagaagatat
     5161 tcttcataaa caaaatcccc aatacgtaaa ttattctgat taaaaaaatt taataacttt
     5221 tcataagcct tgtaaatagt ttcataacta cctttatgat aagcagttac ataaagtccc
     5281 tttggtttga ttgatacagg atatttcttg tactctttat taacttttgt aaataaatat
     5341 gaatatgatt caaagtcttt attaattata tcttcacctt taatgattga gccaatcgta
     5401 ccatagtcac tcaattcata gttgtccata aagttaatac attcatataa aaaattttct
     5461 tcctcatctc ctaaaaactg ttctttatat gtaataggtg ttttgaccaa gtactcttcc
     5521 tcatgttctt ttaaagtaat agtattcgca tctatattac aggcattttt agtgaatgaa
     5581 atagtagatt ccattagagc ttgaatatta tttagttttt caatttcatt tttaatatca
     5641 atggttttct tttctaaaag ctcaattagt aaattaggtg ttcttttgtc tatataagct
     5701 ttaatttctt ttaaaggcat ttttacttcc ctaagtgaag aaattatacc aaaaatgcta
     5761 agttgatggt atgagtagta tctatatcca ttttctttct ttaattctgg tgagaaaaga
     5821 cctatatcat cataatggaa aagtgttttt ttattgattc cacaaatctt tgcaaattct
     5881 ccagttgtaa aataattttt tgaaacttga ctcataatta taattcctcc ttgactatcc
     5941 tgttacagta tagtttatca tagtattttg taaaggagtg tgattatatg aaaaaattaa
     6001 ttcatttttt aaaaccatat agagttttaa ttgttgtagt tttgatattt acgtttttac
     6061 aaacacttgg aactttgtat ataccaacac ttacagcaaa tatagtaaac aatggtgtag
     6121 taaagggaga tatagattat attgtaaaaa caggtttaat aatgatgata gtagctggta
     6181 taacagcttt atcagctgtg ttagtttgta aagtatcagc aaatctttca tctggatttt
     6241 gtagagatat tagagaagca gtatttgtaa aatcacaaga tttatcaata aatgatttta
     6301 ataatatagg tacagcatca atgattacta ggagtactag tgatattact ttaataggtc
     6361 agtctgtgtt tatgtttatt caattggtat taccagcacc aattataact gtttctggtc
     6421 tattcttagc atattctata gataaagcaa tgacaattat tattgttgta gtaatgtttt
     6481 tattcatgct ttcagcattt ttagttggta aaaagcttat aaagttgttt aaaatgatgc
     6541 aaataaagat ggacaatatg aatagggtat tacgtgaggt agtaactgga gtaagagtta
     6601 taagagcttt taatagggag cattttgaaa aaaagagatt tgatagaact gcaatagatt
     6661 attctgaaac agcaatttca ataaataaag tatttgcagt acttatgcct attgtaatgt
     6721 taataatgaa tttaggtata gtaagtatta tttggtttgg tgggatgaga gtcagtaatg
     6781 gaaatatgga gataggtcat ataatggcac ttattgaata ttgtatctta attttattct
     6841 atttaatcat gggagtaatg gtatttatgt acatacctag agcaggtgca tgtgcagata
     6901 gagtaaatca gatattagat atagaacctg aaattgtaga tggaaatggt cgtaaagata
     6961 ctgtctcaga gaggggacat ttagtattta aaaatgtaac attttcatat gcacaatcag
     7021 aagaacctgt gttaaataat ataacatttg aagcaaaatc tggagaagta actgcaatta
     7081 taggaagcac tggctcaggc aaatctacta ttgcaaatat aataccacgt ttttttgaaa
     7141 ttcaatctgg agaaatatct ataaatggtc aggatgttaa gaagattcct caaaaagaac
     7201 ttagagataa gattggattt gtgccacaaa aagctttttt atttagtgga acaatagaag
     7261 aaaatataag gtatggaaaa gaagatgcaa gtattgaaga aataaagcat gctgcaagta
     7321 tagctcaagc tgatgaattt atttctgata tggaagacaa gtatgactca tttgtagcac
     7381 aaggtggaaa taatttgtca ggtggtcaaa aacagagaat atccattgca agagcgttag
     7441 ttagaaagcc tgaagtatat gtctttgatg atagtttttc tgcattagat tttaaaacag
     7501 ataaaagact tagaaaagcg ttgaaaaatg aaatcaaaga ttcatcagct ataattatag
     7561 cacaacgtat aagtacaatc atggatgcta atcagattat agttttaaat gatggaaaaa
     7621 ttgtaggtat tggaaaacat aaagatttac ttgaaaattg tgaagtttat aagcaaattg
     7681 cggattcaca acttagtaaa gaagaacttg cataaaatga agaattaatc tgatatttgg
     7741 aggtgtatga tatgagtgaa aaaagaatag atgaacaaga tatagaagta cttagttttg
     7801 aagatggcat gtcagcagaa aaagttaagg atgcaaaaaa aactactaag agattattaa
     7861 aatatatggc aaaacaaaag tttaaaatat taattatttt tatttcagta ttgataagtt
     7921 cagcgcttac agttttagca cctatggtta tgggaaaagc tattgaccaa ctatttaatg
     7981 gaataaaaac tgcagtccaa acaggtacaa aattcagtgt gaattttagt actatggggg
     8041 ggatagtatc aatacttcta gggttgtact taattagtgc agtatttata tatattcaac
     8101 agtatattat gtcaggagtt gcacaaaatc tagttcttag catgagaaaa gatttaagtg
     8161 ataaactcaa taaacttcct cttaagtact atgattctca taaaaagggg gaaacactta
     8221 gtatagtaac aaatgattta gaaaaagtag cagatagttt acaagaagga ttaatgcaac
     8281 ttattacagc agtagttaca gtagttggtt ctattgtaat gatgataagt ataagtgtac
     8341 cacttacaat agtatcagct ataacattac ttgtttcatt gggtattact gttgtaattg
     8401 caagaaaatc acaagaaaga ttctctgaaa atcaaaaggc actaggagag ttaaatagca
     8461 atatagaaga gatatttact ggtcaaatag tggttaaagc atttagtaaa gaaaaggata
     8521 ctataagaaa ctttaaaata ttaaatcaaa atctttataa tgcaagtaga agagcacagt
     8581 tttcgtcata tgcaatatct ccaattatta gatttataaa cctaattggc tatgttatta
     8641 tagcagttgt aggtgggata tttgcatcaa ctggagcaat gactcttggt agtatacaag
     8701 cgtttattca atatgtaaat caagcatcag agcctactac agaaatatct tatattgtaa
     8761 atatgcttca agcagcaata gcatctgcag agagagtttt tacagttatg gatgaggtgg
     8821 aagagataaa ggatgaagaa tcttcaaagg ttattagcat gcctaaaggt aaagtacaat
     8881 ttgagcatgt taaatttgga tatagtgatg attttattct tatgaaagat attaatatca
     8941 atctaaatgc aggagacaag attgcaattg ttggacctac aggagctgga aagacaactc
     9001 ttgtaaattt acttatgaga ttttatgaga tacaaggtgg aagaattact attgatggtg
     9061 ttaatataaa agacttaaag cgtggagaac ttagaactat gtttggtatg gttcttcaag
     9121 atacatggct gtttaatgga agtataaaag aaaatattgc atatagtaag tcagatgcca
     9181 caatggatga aattgtaaga gcagcaaaat cagcaagagt agaccatttt ataaggactt
     9241 taccacaagg gtatgacact atattgactg aggatgcttc taatctatct caaggtcaaa
     9301 agcaacttct aacaattgcc agggcaatac tttcagaccc atcagtattg attcttgatg
     9361 aagcaacatc tagtgtagat actagaacag aggtagaaat tcaaaaggca atgaataact
     9421 tgatgaaggg aagaactagt tttgttattg cacatagatt atcaactatt cgagatgctg
     9481 atttaatatt agtaatgaaa gaaggcacta ttattgaaca aggaagccat aaggaactta
     9541 ttgaaaagaa aggattctat gaagaacttt ataatagtca attttcaagt gaatatgatg
     9601 aagaagttgt atgattatca agttttaggc aactaaatga ttgataaata ttaagttaaa
     9661 tatagtaaca tgtaaggtta tatggaataa gagtatttct ctatttcata taaccttttt
     9721 tatttatcaa aataagataa aattttcttt ctaaattaga ttgaagaaag tatttatcaa
     9781 tataaaagta aaataatcat tattattagt gaagtaaata ttgtttgcca catatatgcc
     9841 ataaagatgc acttatttta cggagattta acaaagtagt gtaaagtata ctctaaatat
     9901 aaaataaatc aaatagagtt aaataaattg agaggtgata taaatagtga ataaaaaatt
     9961 gaactataga tttgaaatga agcacaaaat aactgaagca gatgtgttag ctttaaaatc
    10021 aagactctat ccaataatga agaaagatga aaatgcatct aaagatggaa aatatcttat
    10081 taggagttta tattttgata ctcctgaaga taaagcactt ttagataaat taaatggcgt
    10141 tgcaataaga gaaaagttta ggataagatt ttataataat aactgctctt atataaggtt
    10201 agagaaaaaa atcaaacatt acaacatgac atcaaagcta tcaactaatt taaccaaaaa
    10261 agaagtaata aatatactaa acaatgatat tgaatttcta aaagagtcga caaattgttt
    10321 attaagagaa ttttatttga aattaaaatg tgaaagatta gaggcaaaaa gcatagttga
    10381 ttacaataga gaagctttta tttatccagt tggaaatgta agagttacaa tcgatagtga
    10441 tattaaaact tctgtaaatt cgatagattt atttaataaa gatttgccga ctgtttcggt
    10501 aattgatgag aatatgactg tattggaggt taaatatgat gaatttatac cagattttat
    10561 aaaggattta attcaaatta ataagactac ttctacagca gtttcaaagt atgcttcaag
    10621 tagattatat atttaagaat aaaggctatg atttatataa agatatacat aaataaggtt
    10681 aaaaaacaaa taggttaaaa ataaataaaa taaaaaataa ataagttaaa aataggaagg
    10741 atgattttaa ttatgacatt taatgatatt tttaaatcaa gttttataga aaacgtatca
    10801 ggtttttcat ttgtggacag tgctttagca ctaggttcag cttttttagt aggtttgttt
    10861 atatatatgg tctataaaaa gacttatatg ggaattatgt attctcgacc attcaatgtg
    10921 tcgcttgtag cacttacaat gcttacaact tttgtaatat tggcggttac ttcaaatgta
    10981 gttttatcct taggtatggt tggtgcttta tctatagtaa gatttagaac agcaattaaa
    11041 gacccaatgg atttagtatt tctattttgg tcattgggtt caggtatagt tttaggagca
    11101 ggtttgattc ccttagctgt catgggttca ataattatgg gattgattct aatattcttt
    11161 tcaaataaaa caatatctga aactccatat atattgatgg taaattgtaa taatgaagat
    11221 tcagaagata tagcaactga taaaattaaa aaagtattta ctaaatatca aataaaatca
    11281 aaaagtgtta caccagaaaa aggaatagaa ttagtgtttg aagtaagaat gaaatatgga
    11341 gagacaagtc ttataaatga cttgagtcaa gtagatggag ttactaatgc agttcttgta
    11401 agctataatg gtgactatgt agcatagaga ggagtaatct tttatgaaag ataaaaaatt
    11461 tacccttctt atctctatta tgattatatt tttatgtgct gtagttggag tttatagtac
    11521 atctagcaac aaaagtgttg atttatatag tgatgtatat attgaaaaat attttaacag
    11581 agacaaggtt atggaagtta atatagagat agatgaaagt gacttgaagg atatgaatga
    11641 aaatgctata aaagaagaat ttaaggttgc aaaagtaact gtagatggag atacatatgg
    11701 aaacgtaggt ataagaacta aaggaaattc aagtcttaca tctgtagcaa atagtgatag
    11761 tgatagatac agctataaga ttaattttga taagtataat actagtcaaa gtatggaagg
    11821 gcttactcaa ttaaatctta ataactgtta ctctgaccca tcttatatga gagagttttt
    11881 aacatatagt atttgcgagg aaatgggatt agcgactcca gaatttgcat atgctaaagt
    11941 ctctataaat ggcgaatatc atggtttgta tttggcagta gaaggattaa aagagtctta
    12001 tcttgaaaat aattttggta atgtaactgg agacttatat aagtcagatg aaggaagctc
    12061 gttgcaatat aaaggagatg acccagaaag ttactcaaac ttaatcgttg aaagtgataa
    12121 aaagacagct gattggtcta aaattacaaa actattaaaa tctttggata caggtgaaga
    12181 tattgaaaaa tatcttgatg tagattctgt ccttaaaaat atagcaataa atacagcttt
    12241 attaaacctt gatagctatc aaggcagttt tgcccataac tattatttat atgagcaaga
    12301 tggagtattt tctatgttac catgggattt taatatgtca tttggtggat ttagtggttt
    12361 tggtggaggt agtcaatcta tagcaattga tgaacctacg acaggtaatt tagaagacag
    12421 acctctcata tcctcgttat taaaaaatga gacacacaaa acaaaatacc ataaatatct
    12481 ggaagagata gtaacaaaat acctagattc agactattta gagaatatga caacaaaatt
    12541 gcatgacatg atagcatcat atgtaaaaga agacccaaca gcattttata cttatgaaga
    12601 atttgaaaaa aatataacat cttcaattga agattctagt gataataagg gatttggtaa
    12661 taaagggttt gacaacaata actctaataa cagtgattct aataataatt ctaatagtga
    12721 aaataagcgc tctggaaatc aaagtgataa aaaagaagtt aatgctgaat taacatcaag
    12781 cgtagtcaaa actaatacag ataatgaaac tgaaaataaa actacaaatg atagcgaaag
    12841 taagaataat acagataaag ataaaagtgg aaatgataat aatcaaaagc tagaaggtcc
    12901 taggggtaaa ggaggtaagt caataccagg ggttttggaa gttgcagaag atatgagtaa
    12961 aactataaaa tctcaattaa gtggagaaac ttcttcgaca aagcaaaact ctggtgatga
    13021 aagttcaagt ggaattaaag gtagtgaaaa gtttgatgag gatatgagtg gtatgccaga
    13081 accacctgag ggaatggatg gtaaaatgcc accaggaatg ggtaatatgg ataagggaga
    13141 tatgaatggt aaaaatggca atatgaatat ggatagaaat caagataatc caagagaagc
    13201 tggaggtttt ggcaatagag gaggaggctc tgtgagtaaa acaacaacat acttcaaatt
    13261 aattttaggt ggagcttcaa tgataataat gtcgattatg ttagtaggtg tatcaagggt
    13321 aaagagaaga agatttataa agtcaaaata agattaaaat tgaatataaa taattttaat
    13381 actgttaagt ctaaatattt acttagcagt atttttttag aaaataatgt gtttgattaa
    13441 taatataagg taataaattg ataaaaatta aatacagcat attaataata cataataaag
    13501 taataaaaaa actgtttaag tataataact tatatttaga cagttttttt catttataaa
    13561 taatataaat gaaattgaaa acatttgaag gatagaaatt ggttggataa atgtataaat
    13621 tacttcacca aaaaataatg acaagagttg taactaagtt gtaataaaat ggtagttatt
    13681 tagcataaaa taatgtatca tagaagtata ttataaaatt aataaaataa gatttttgta
    13741 agaaaactgc tggcttttga tatttagagt gagtagttaa attatgttta ggaggggtag
    13801 aatttgttgc aaaaagtatt atggaaattt ttaaagctag tagtcggttt atttatatgt
    13861 tcagttggta tagtattgac gataaattgt aaccttggat tatctccctg ggatgtattt
    13921 catcaaggac tttcaaatca tataggaata actataggaa ctgcttctat aattgtaggc
    13981 tctatagtag ttattgctga tgtcgttctt ggagaaaatg taggatgggg aactgttttt
    14041 aacatgctac taattggatt ctttatggat ttacttttat atagtaattt aattccagag
    14101 gctgatagtt tatttgtagg aataattatg ttgatattag gattggttct tttgtctgtt
    14161 ggaatggtat tttatatggg aagtggttta ggaagtggac ctagagatgg tttgatggtt
    14221 gcaattcaaa agaaaacagg aaaatcactt aaattaataa gaggtactat agaagttgga
    14281 gcattaatag taggattttt attaggtgga aaagttggta taggaactat aataagtgca
    14341 tttggattgg ggtatttcac acaaatggta tttggcttat ttaaattaga ttgtagtaag
    14401 attaaacata gatttatagt agatgatata aaatttatca aagtgtatat gagtggtgag
    14461 aaaaaagctg ctgttcaaaa tatagttaag taatttaaga ataaattcaa cgaaatgtta
    14521 agtattttaa attaacattt tatttagatg gataaattga atattaaaag aatattaaaa
    14581 tatgaggatt gctagaacac tataaaatat agatttctag caatttttta tttgttcaat
    14641 gtttttttag tactagaagt cttattctct atttaatgaa tgaattaatt tataattaga
    14701 gatactaaac tttatagata ttaatataaa cacctttaaa ctgtgtattt tccaaagcct
    14761 ttaaaatgct tgtatttatt ctaaataaaa aaataaaaaa atgtcaaaaa aactcttgta
    14821 ttatatccaa attttatata taattaccta tgttgtttag aaattttaaa ctcaatgttt
    14881 agtaaacaaa atgtttagta aaataaaaca gaaactagga ggtgcaacat ggatataggt
    14941 gaaaagataa aaagattaag aacagaaaaa caactgaccc aggaagagct agctaataga
    15001 tgtgaattgt caaagggatt tatttctcaa ttagaaaata acttaacatc accatccata
    15061 gctaccctta tagatatact tgaaatacta ggtacaaatt taagggaatt ttttaatgaa
    15121 atagatgatg aaagaatcag ttttacaaaa gaagatatgt ttgaaactga agatgaagat
    15181 ttaaaatata aattaaagtg gttaattcca aattcacaaa aaaatgaaat ggagccaata
    15241 ataataacat tgtatccagg aggacagtat aaagaagaaa agccacatga aggtgaagaa
    15301 tttggatatg tattagcagg ttcaatatat gtccatatag gcgaaaagaa aaataaagta
    15361 aaaaaaggag agagttttta ctttagacca aaagcaaatc actatatatc aaacgaaggc
    15421 aaaactacag caaaagtgat ttgggtaagt acgccaccat cattttagaa tagaggtgtt
    15481 taataattgg tagaaaatat aatagaatta agggatatat ctaaacatta tgaggaactt
    15541 actgttttag ataatttcaa tttagatata aagaaaaatg agtttttaac attacttgga
    15601 ccaagtggat gtggaaaaac tacaacatta aaaataatag caggatttga gtatgctgat
    15661 gatggtaaag ttttatttga aggtaaggag ataaataact tgccaccata tgaaagacaa
    15721 gtaaacacag tatttcaaaa atatgcttta tttccacaca tggatgttta tgaaaatgta
    15781 gcttttggat taaaaattaa aaagactcca aaagatgtaa tagatgctaa agtaaaagag
    15841 gttttgaaac ttgttgcact agaaggtttt gaaagacgac aagttgaatc attgagtgga
    15901 ggacaacaac agagaattgc aatagcaaga gctctagtca atgaaccaaa ggtattatta
    15961 ctagatgaac cacttggagc acttgattta aagttaagac aagagatgca aatagaatta
    16021 aagagaatcc aaaaaaaatt aggaataaca tttgtttttg taactcatga ccaagaagag
    16081 gctcttacta tgtcagatac tattgttgta atgtataagg gcaaaattca acaaatggga
    16141 acaccacaag atatatacaa tgaacctaaa aattcatttg tagctaaatt tataggtgaa
    16201 agtaacatat ttgatggaat tatgatagaa gattataaag ttaatttctg taatagagat
    16261 tttgagtgtg ttgataaagg attcgaaaaa aatgaaaata tagaagtagt aataagacca
    16321 gaagatataa aaatggtaaa gccagaagaa ggaatgttaa aggggaaagt tacatctaca
    16381 gtgtttaaag gagtgcatta cgagatagaa ttgaatgaaa atggaagatt gtggatactt
    16441 cataatacca aaaatgctga ggttgggact gaactaggca tggatatata tccagaagat
    16501 atccatataa tgagaaaaga gaataactaa gatgaaaaga aaatcttttt tagcatatcc
    16561 atatgtagtt tggagtgcga tatttgtaat aattccttta atattagtgg tattttttag
    16621 ttttacaaaa gaatcaggtg gaggttatgc ctttacactt gagaattata aagaagtaat
    16681 tgaccctata tatatgaaag tttttgggcg ttctattctt ttagcaggag gagctacatt
    16741 aatatgcctt atagttggtt atccagtggc atatataatt tcaaaggctc gtgtaagtag
    16801 aagaggttca ttaatattat tgtttattct tcctatgtgg atgaactttc ttttaagaac
    16861 atatgcatgg gttgctatat taggaaaaaa tggactttta aatacatttt taggttggtt
    16921 tggaatacaa ccattagcaa ttttgtatac taattttgct atacttttag gtatggtgta
    16981 taacttttta ccatttatgg ttcttccaat atatacagca ctatcaaaga tggataatga
    17041 tttgataaac gctgctcatg acttgggtgc aaataatatg acagttttta gaaaaattat
    17101 atttcctttg agtttgccag gtgtaatgtc tggaataact atggtattta tgccagcagt
    17161 cactactttt gcaatatcaa gactgttagg tggaggtaaa ataatgctgg ttggagattt
    17221 gattgaacaa cagtttacag ttgttggaga ttggaacttt ggttcagcta tatcgatatt
    17281 tatgatgata gtaatattaa tatctatgtc tataatgtca aaatttggag atgaatctga
    17341 caaagaaggc ggtgggttac tattttaaaa ttaaagaaaa taacttctaa tatatattta
    17401 gcattagtat tcttattttt gtatgcaccc atatttgctt tggtactatt ttctttcaat
    17461 gattcaaaat caatggcaag atggaatgga tttacatgga agtggtatgg tcaattactt
    17521 caaaatgaga gtataatgag tgctttatat tatactattg taatagctat attagcgtct
    17581 gtaatatcta caatagtagg aactataagt gctataggaa tacataagat gagaggtaag
    17641 agtaagaaat taatactaaa tgtaaactat cttcctattt taaatacaga gatagttaca
    17701 gcagtagctc ttatgagcct gtttgtattt gtaaaaatgg aatttggatt tacaacaatg
    17761 ctattggcac atataatgtt ttgtttgcca tacgttatac tttcagtttt gccaaaaatg
    17821 aagcaattac ctgataatat agaagatgca gctatggact tgggagcaac acctatatac
    17881 gctcttagaa aagtaatttt accacaaata aaaccaggta tagtttctgg atttttaatt
    17941 gcatttacta tgtcaataga tgactttata ataagtttct ttaatgctgg aaatggtgta
    18001 agcaatcttt ctatagaaat ctatggaatg gctagaagag gtataaaacc tgaaataaat
    18061 gcattatcaa caataatgtt tgcagtggtt ctaggtttac ttttactagc taataaaaaa
    18121 gaatctatag taagggggat taaataatga ttaagtttaa aaaaacaata tcccttatag
    18181 ctgtaggtat gatgacagtt actttattta caggatgtaa taaagcaact gattctacaa
    18241 aagttttaaa tgtatataat gttggagatt atatagatga gtctcttata gataagtttg
    18301 aaaaagaaac aggaatagat gttcaatatt caacttatga tactaatgaa atgatgtatc
    18361 aaaaagtaaa gagtggtagt acacactatg atttagtatt tccttcagac tatatggtag
    18421 agaaaatgaa aaatgaaggt cttttggaaa aattagattt taagaatata cctaatatga
    18481 aatatttaga taagagtttt ttaaatcctg tatacgacaa aactaatgaa tatagtgttg
    18541 catatatgtg gggaactctt ggaatattgt acaataagaa agaagttaaa gaccctatgg
    18601 atagctggaa tatactttgg aatccaaagt ataagggaaa tataatgatg tttgactctg
    18661 ttagagatac tatagggata actcttaaaa aattaggata tagcatgaat agtgtaaatc
    18721 caaaagaaat aaatgaagct aaaaatcttt taatgaaaca aaaggattta gtattggctt
    18781 atgtaaatga tgaaggtaag gatagattgc ttggtggaga agtggcaatg ggtatgctgt
    18841 attctggaga tgcagttacg ctaatggagc aaaatcctga ccttgattat gctataccta
    18901 aagagggaac taataaatgg gtagatgcta tgtgtgtacc tacaacagca caaaataaga
    18961 aagaagctga gctgtttata aactttttat tagacccaga gaatgcaaaa gtaaatgctg
    19021 agtacattgg gtattcaaca cctaatacag gagcactaaa attattagac ccagaaataa
    19081 ctcagaatcc tgttgcatat ccatctaaag aagtacttga taaatgtgaa acttttgaag
    19141 atattggtga aaatataaaa ttatatgaca aggcttggat agagttaaaa tcaaaatagg
    19201 gtcttgttgg tttaagtaaa tttacttaat aaatacttaa taaaactgtt cttttttaga
    19261 aaatttattt tctaattatt gaacagtttt attttttgtt taggtcatgt ttttattttg
    19321 agtttatttt tatgagaatt taaagtacag taagtttcat tgattactat ttttataata
    19381 ataactataa agaggttgtc tcgaaattga gataagcctc tttttcattg ttgaaatgat
    19441 gaaaacatgt ttgatttcat aaaaaaagag tgattcatga gctaaagaat ttgctttgag
    19501 acagtatttt ttattaagtt atattcatat gcaatttaaa tagtttatta ataaatgaaa
    19561 acttatttgt ataatgattt taataataat tagaaaaaat gggcaactta atagaataat
    19621 tggaggtgta aatttataaa aaatgaatag gataaataaa aaaatagatt tattaatatt
    19681 ggctttgctc gtttttatat ttttaattgt tggaatagta atactatcga ttaggacaga
    19741 aaataaattg aatgtgttca taatgttagc aataattttt tttataataa tgttaactta
    19801 tttatcaaat tcagtagtgg ggttgataac aagttcaata attatattta tgtatacatc
    19861 ctatatttta tataacaata ttactcacaa tatggatgtt gaatttatat catatatgtg
    19921 gataatagca acaccagtat cttcaattat aatgggtaat ctgaacaaaa gcataaatga
    19981 attgcaaaat acaaataaaa aactatctga acagtataag gaattagtga ctattgatag
    20041 tgaaactgga cttagaaatt taaagatttt ttataatgat gtaaatatgg agataagtaa
    20101 atctattaga cataatactg atttttctct tatgatagta aaattaccat actacggaaa
    20161 cctacagact atatttggtg aaaacaaaac taataaaata gtaaaatata ttggttcaaa
    20221 tattatagaa tgtacaagaa atgaagatat aatttatagt ttacaaaaag atatgatagg
    20281 gatactaatg ccaaatacat ctttggaagg ttcgaaagtt gttaaagaca gaataaagaa
    20341 aaggataaaa gaattaaatt tagatttaaa caatagggga aaatatgtaa atatagatgt
    20401 caaaattgca tttttacaat ataagagtag ttttggagat agtataaatt ttaagaatat
    20461 agttgaagag gaacttcaat atgatgttta aaatatttaa taaaagttta ttaaaattag
    20521 taattgtatt atcaatagta ctgacaatat caaattttag cttattttca tatgcatcag
    20581 aaattacttc cagcacagat aaggtattaa tcatatatga ttctaagaaa gaaacagctt
    20641 ataatagaga tattttaaat attatgagaa ctctattggg aagatttagt tcagatatag
    20701 agctattaaa attgagtaac tatgatggtg agataaatga aaattattac agtcatatat
    20761 ttattttagg aatcaatgaa aactcatata ataatgataa aaacactaaa aacttgatta
    20821 gttccttaaa ttcatataaa gtaactatat gttggttagg atatggtata gaaaatcttt
    20881 tggaacataa aaaatacaat ttagactatg taggaaaaac aaacaatata gttagtgtaa
    20941 attatagagg taaatcatat aacttggatg aacattatgt ttttaacatt gtagaatcaa
    21001 aagatacaag taataaagta ataggaagta ttaatgatac cttaaataag tatccatata
    21061 taataaatga taaaaatctt ttttatgtat caaaattaga tttagatgga gttttatttt
    21121 atatattttg cgattcactt aatgatattt ttaatattaa gacgtttgat aaaggacgta
    21181 tatttgtaag aatagaagat gtacatgctt ttagagaacc aaaaaatctt gtagagatag
    21241 cggattattt aagtagtaag aatataccat ttacgattgc attaatacca gcatatgtaa
    21301 atcctaaaaa tcataaagta ataactttat cagaaagccc tgaaattgtc aaagcaataa
    21361 aatatatgca agataaaggt ggaactgtga tattgcatgg atatactcat caatacaaga
    21421 aagaagaagt ttctggagaa gggtatgaat tttgggatgg taaaaaagat gaacccttaa
    21481 aagaaaatat gaagatattt gtcaaagata gagttttgaa tggtcttaga gtatgtatag
    21541 agaatggaat ttatcctctt gcatttgaag cgccacatta tgcaatggaa tcagagggat
    21601 ataaagaatt aaaaaaatat ttttctactt atatggggca acatcaaaat aatgataaaa
    21661 aatttagcac aaatacatat ccttatatta ttagggatac agaggagttt aatatattta
    21721 tacccgaaaa tttaggatat atagacccag aagacaagtt tacttttcaa aatataaaag
    21781 aaaatttaga taaattatct attgtaagag gttttagtgg agggttcttc tttcattcat
    21841 atttaaacat agagtatcta aaaaacacaa tagaatattt agaaaaacag aatatagaat
    21901 ttatgaattt aagagatttt aataattggg taaaagttga tgaaatacaa ataagaaata
    21961 atggagatga aattatagtc aactatgata aggacttaga tgaaataact aaaagtgata
    22021 ctagatttaa atacatatcc aatattagta aaattttaat attcatagta tctataagtg
    22081 ttttaatatt tgtgattata tttatatatt ttaagagaat agataaaaag aaatttttaa
    22141 aataaatgat tttatattag aatcaatatt ttaaaatatt ttaggagaaa tttatgagag
    22201 atttaaatgt atcagagtat ttgttcgtat ttagtctgtt tagtatatgg tctttattgc
    22261 ttataaatat tattttagct atgggtggat atatatttta ttttaaaaac tttgataaag
    22321 aaataaaaga aattgatgaa tatcctatga tatcaatact tgttcctgct cataatgaag
    22381 ctaaagtgat aggtagaact gtggagtctt tgttattact caattatcct aagagtaaga
    22441 tggaacttat tgttataaat gacaattctt ctgataatag taaagaaatt ttagaaaata
    22501 taaaggatag gtacaacaat tataatttca ctataataaa tacagatagt ttaacaggtg
    22561 gaaaaggaaa gtctaatgca ttaaatattg gatataaggt ttcaaaagga gattttatag
    22621 ctgtttatga tgcagataac acaccagata aaaatgcact tagatatcta gtacaaacta
    22681 tagtaatgaa tgatgaatta ggtgctgtta ttgggaaatt taggactcga aataaaaata
    22741 aaaacctatt aaccaaattt ataaacatag aaacattaag ctttcaatgg atgtcacaag
    22801 ctggtagatg gcaacttttt aatctatgta caataccagg aactaacttt atattgagga
    22861 gaagtatcat agaagaaatt gaaggatggg atagtaaagc aattgcagaa gatacagaaa
    22921 taagttttag gatatataaa ttgggataca agataaagct tgtaccacaa tctataacat
    22981 gggaacaaga gccagaaact gttaaggtgt ggataaagca acgtactaga tgggcaaaag
    23041 ggaatatata tgtattaatg aaatatataa aaaatatatt taaacaggga agaaataaga
    23101 ttgtgtttga tatagcttac tttttttctg tatattttct atttttaaca actgtaatta
    23161 tatccgatat tttatgtgtt ttaagtatat ctaaactagt agaaatttct attccaatta
    23221 acttttttct aatatggata ctatcttatc ttttatttat tatagaagtg agtataagcc
    23281 ttactataga aaaaggtgaa gcaacaattg aaaatatatt catagttgca ataatgtatt
    23341 ttacatactc tcaactatgg ctgtttgtag ctataaaagg gatgatagag tatctaaaag
    23401 atataatttt taaaagagag gttaaatggt ataaaacaga aagattttga ttcatgtaca
    23461 ggaggaatta acatgaaaaa atttattata tcaattatat ctttagtatt gtttttttca
    23521 aatatatcac ttatatacaa agtaaatgct gatgagacta aagttaaaaa ttataagttt
    23581 gaaagagata taactattga tggtgttata ggaagtaata gtactttttt tgaagtaaat
    23641 aaaaattggg atattgaaga agttttattg catcttaatt ttagtaagag ccaaatatta
    23701 aatggagatg tttcaagttt aactgtattg ataaataatg taccaataaa atctattaaa
    23761 ttaaatgcta agacaaatta taaaaataca ttagaagtat tagttccaaa agattatata
    23821 attcaaggat ataatgaaat aaaaataaaa acatacaaaa caatatctga taaaatttgc
    23881 caagatgatt ccaatactgg gaactggatg gttattcaca aagaatcata tatatcaatt
    23941 agatataagc agaaaaaggt ggaaaactca ataaatgaat atccgtatcc atatgcagaa
    24001 attgaaaata atcataaatt agatacaact atagtagtac cagataatat gactagagga
    24061 gagactacag cagtgtttaa tttggcatca tcatttggaa aaataacaaa aaatgatgat
    24121 ttaaagttag atgttaaatt atattcagaa atgaaaaatt ggtcagatga taatattata
    24181 tatattggta aacctgaaaa tacagcagaa gaaattttgg atattttaag tataaaggaa
    24241 caaaccctat tatcttcaaa ctgtataatc aagcaagtag attcaccata caataagaat
    24301 aagaagatga tggtggtaat tggtagtaat gaagatgatt taataaaggc atcaaattta
    24361 ttaatagaaa atagactttc taaccaagtt ttatcatcat cagtgcttgt aaacaaagag
    24421 actaatataa aaattaatag ggaacaaaaa ttaaatttag gtcatttaac attaaaagac
    24481 ttaggatatt ctgacttttt gttggaagga gcatttaatc aacaagcttt gtttgatgtc
    24541 aaaataccaa cgggtaaggt attagatgat ggttcaaaga ttatcttaaa tttaagatat
    24601 tctgacaatt tagattttga aaaatcttta gttacagtgt ctataaatga tgttattgtg
    24661 ggaagtaaaa aattggatag gtcacattct aataatgata agttagaatt aaaaatacct
    24721 aaagatatag ataataaaaa ctattatcaa gttaaattga catttaattt gagtataaaa
    24781 aattcgaatt gtgttactag agagagtaat aatccttggg catatgtatc aaataattct
    24841 tatttggcgt tatctacgaa agaaaatgaa accttatctt ttgaaaatta tccgtatcca
    24901 tttgttagag atgatgagtt taatgattta actgtgataa tgccagatta ttcagggtca
    24961 caagctatga cctggatgtt tagattaggt gttactttag gtacgaatat aaattcacat
    25021 aatggaaata taaatgtaat tagaggaaaa gaatttagtg acaagtataa ggatactaat
    25081 attgtagttt ttggagtgcc acataataat tctgttataa agatgttaaa taataattta
    25141 aatataaagt tcgataaaaa ttactccaat tttatatcaa atgataagat aagctttatt
    25201 gatgattatg gaaaaaatat ttctaccatt caattaataa aatcaccata taataatcaa
    25261 aaaaatatca tggtaattag ttcaatgaat gaaaaaaact tatatttagg aatggattat
    25321 ttgttgaata aaagtaaagt taatgattta aaaggagata cattgataat tgatgaatac
    25381 ggagaggtag aagatttagc ttataattta aaacctaaga aagaagtaaa agattcaagc
    25441 tggaatatga gtataaataa gacaactaaa gtatttttaa tgatttcttt tattactata
    25501 attgtagtca tgatattatc aatgctatat ataaaaaaat ataaaagaag ataatatgat
    25561 aaatttatta ttttaaaaga ggtatataat ttgaggaaat atatgaataa atttaaatta
    25621 aaaagaagca ttttaatctc attaataatc ttaattttta tatttatagt gtactttgta
    25681 agtattgagt taaaagatgg gagtctagta aataatcaat ataaaggtaa gattaaatcg
    25741 gccaatttat ctgtagatta tgaaataaat caagttatga aggatataga taaattggga
    25801 ttgaatacag taaatgtacc tataattata aatgtagagt ccattaattc tgatattatg
    25861 tcaatagata ataatagtaa agaaaaggca attaagttaa taaaaaaatt aaataaaaaa
    25921 ggtatttcaa caatattaga ggcatatcca tgggttgaaa atggaaaatt gtatgagact
    25981 gactggaacc ctataaataa aaaaagattt ttttatactt ggcaaaatgc catattaaat
    26041 gaacttataa ttgatgtagc aaatccactg gatgtaaatg ttttaaatat tggttctaat
    26101 tttgtacatt tggaagaata tcaacaaaat tggggtgaaa taatagattt tgtacaatcc
    26161 aaatttgatg gtcttgtaac gtacagaact aattggtggt atacaaaaaa agatgattca
    26221 agtagtaagt tgttttatga acaaaaattg aacaataatt tttttgataa gttagatttt
    26281 atatctatag cagcatattt tgagttaagt aataaacctg ttaatacagt ggatgaatta
    26341 atttctgcct tacactcatc tactgtaaac aatagaggac aaaatataaa acaagaaata
    26401 tataaccttt ataaagctca tagaaaacct atattttttg gagaattagg tttttcaaat
    26461 agagaaagtg caagttctca gccgtggaat catactcctt caaaagctgt aaatggcgaa
    26521 gaacaagcaa ggtgttttga ggcatataag aaagtttttg aaaatgaaga ctggataaat
    26581 ggtttttctg tgttttgtgt aggtaaaata gatgacgaaa aaaattttta cccatcaaaa
    26641 gagagtataa aagttattaa gtcttggtat gaataatttt aaaagtgtag aatttatata
    26701 gattaaatag atgattagga gagatataaa tgaatgacat caaggttatg actgtatttg
    26761 gaactagacc tgaagctatc aaagtagcac ctttaataaa ggaacttgaa aaaagagaga
    26821 atataaaaag tattgtttgc gttacagctc aacatagaga aatgcttgac caagttatag
    26881 aaaccttcaa tataaatgta gactatgatt tggatataat ggaaaaagga caaagcctga
    26941 atgatataac atgtaagatt ttaaataaac ttcccttgat tttaaacaag gaaaatccaa
    27001 atataatatt ggtacatgga gatacaacca caactctagc tacaagtttg actgcatttt
    27061 ataataaagc tcttgttggt catatagaag ctgggcttag aacctatgat aaatattctc
    27121 cttttccaga ggagttaaat agacaactta caggtattat tgctgatatg cactttgctc
    27181 ctactaattt ggctagaaaa aacttgattt ctgaaggaaa acctaataat aatatatttg
    27241 taactggtaa tactgctatt gatgctttaa agatgacaat aaaagaaaac tataatcatc
    27301 ctataattga tgagattggt aacgatagga tgattttgtt aacctctcac agaagagaaa
    27361 atttgggtaa acctatgaaa aatatattca gagctataaa gcgaatagta gatgattttg
    27421 aagatgtaca gatagtatat cccatacatt taaatcctaa aatacgaact atagctgatg
    27481 agatatttgg aaaatttcct gaaaaaattc atattataga gcctttagat gtagctgatt
    27541 ttcataactt tttaaataaa tcttatatga taatgactga tagtggagga atacaggaag
    27601 aagctccatc attaggtaag ccagtccttg tgttgcgtga taaaacagaa agaacagaag
    27661 gaattgaagc aaagacactt aaattagtag gtactaatga agatagaata tataattcag
    27721 tgagcgattt attaataaat aaagatagct atgttcagat gagtaaagca tctaatccat
    27781 atggagatgg aaatgcaagc aaatatatag tggatataat aattaaaaaa tttaattgca
    27841 aatatttaaa ttagataagg tctaaaacat taactaattg atataagatt tatgataata
    27901 ttaaggaata caatttttaa gggggagtaa tatggctaga aaattaataa aaaatttagg
    27961 taaaagtaaa agtgtaaaga gagtgaaact tttatttaag aagattttta ttactgtatt
    28021 catagtagca agtatagttg ctatttttaa tataacgaaa tattttgagg aattatacaa
    28081 agtaagggat ttaaaaagta ctaaaattga atactatatg gatgtggctg atgaagcagg
    28141 agatggaaag gtccaattaa gttggaaggc cttacttgct atagacatgg taatacatga
    28201 tgaagattta agtaatataa aaaagaaaga cacattggat ataggggaaa agtttatagt
    28261 agaagataaa aatgataaag gcgaaaaggt gtataaggta aaaaagttta ataaggtatt
    28321 aagcgaattg aaatttgact cttctcaaaa aagtagagca agaaaataca tgaaagattt
    28381 ggaatacaca taccttggaa ataaacaatt agatagtagt gatgaaaaaa ttaaatttat
    28441 aaagaagtta gaagactcag ctataagaga atatattgat tatggaatat tgccctctgt
    28501 aacaattgga caagctatat tagaatctgg ctggggaaat tctaaactta caaaacagag
    28561 taataattta tttggtataa aagcagataa agcatggaaa ggaaaaagtg tagaaatttc
    28621 aacttcagag cattataatg aaaaaattgt agctagtttt aggtcgtata attcattaca
    28681 agattctgtc aaagaccaca gtttattttt aattaataat aaaagatata gaaaacatgg
    28741 gttgtttgag gcaaaagatt atattagtca agctcaagca ttagaaaatg caggatatag
    28801 tacagcagaa gataaaaaag gaaatcgtat atatgcagag ctattaatag atattattag
    28861 gagttataat ttgcaattga tagacaataa agttgagaca aaataattgt taactttata
    28921 tgaggagata acaagataag attcttttct acaaaaagaa tagaaaaatt gtaaaaaaaa
    28981 ctaaaaaaat gtattgactt gttatttaaa aggtgatatt attattactg tactcgaaag
    29041 agaacaaaa
//
LOCUS       DAGQWR010000042        28958 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.042, whole genome shotgun sequence.
ACCESSION   DAGQWR010000042 DAGQWR010000000
VERSION     DAGQWR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.042"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(109..270)
                     /locus_tag="MIW95_001574"
     CDS             complement(109..270)
                     /locus_tag="MIW95_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3150905.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFD"
     gene            complement(444..1310)
                     /locus_tag="MIW95_001575"
     CDS             complement(444..1310)
                     /locus_tag="MIW95_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY3150906.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNVEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTREYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(1536..2405)
                     /locus_tag="MIW95_001576"
     CDS             complement(1536..2405)
                     /locus_tag="MIW95_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY3150907.1"
                     /translation="MDYKTIILNRKSVREYKNIDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNKSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(2911..3132)
                     /locus_tag="MIW95_001577"
     CDS             complement(2911..3132)
                     /locus_tag="MIW95_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150908.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(3633..4613)
                     /locus_tag="MIW95_001578"
     CDS             complement(3633..4613)
                     /locus_tag="MIW95_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150909.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRVYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(4777..5649)
                     /locus_tag="MIW95_001579"
     CDS             complement(4777..5649)
                     /locus_tag="MIW95_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY3150910.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(5813..6364)
                     /locus_tag="MIW95_001580"
     CDS             complement(5813..6364)
                     /locus_tag="MIW95_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3150911.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSYVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(6384..7286)
                     /locus_tag="MIW95_001581"
     CDS             complement(6384..7286)
                     /locus_tag="MIW95_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBY3150912.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EKMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(7301..9673)
                     /locus_tag="MIW95_001582"
     CDS             complement(7301..9673)
                     /locus_tag="MIW95_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBY3150913.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAEGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(9892..10581)
                     /locus_tag="MIW95_001583"
     CDS             complement(9892..10581)
                     /locus_tag="MIW95_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150914.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMAGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEEFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(10822..12201)
                     /locus_tag="MIW95_001584"
     CDS             complement(10822..12201)
                     /locus_tag="MIW95_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBY3150915.1"
                     /translation="MLNFMNELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGSYFSNINYLFYILAFIAWGGYYNYFYKMDYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLVFSFFRKS
                     IKLISQDKRNYVYLLFALLANTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITKSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     EQDTDSWGD"
     gene            complement(12195..12908)
                     /gene="rgbR"
                     /locus_tag="MIW95_001585"
     CDS             complement(12195..12908)
                     /gene="rgbR"
                     /locus_tag="MIW95_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBY3150916.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDDEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNAAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(13159..13719)
                     /locus_tag="MIW95_001586"
     CDS             complement(13159..13719)
                     /locus_tag="MIW95_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBY3150917.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(13815..14618)
                     /gene="zupT"
                     /locus_tag="MIW95_001587"
     CDS             complement(13815..14618)
                     /gene="zupT"
                     /locus_tag="MIW95_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBY3150918.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(14769..15932)
                     /locus_tag="MIW95_001588"
     CDS             complement(14769..15932)
                     /locus_tag="MIW95_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY3150919.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IEIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(15992..17179)
                     /locus_tag="MIW95_001589"
     CDS             complement(15992..17179)
                     /locus_tag="MIW95_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150920.1"
                     /translation="MEAITAFCLVLISLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTIVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(17542..19242)
                     /locus_tag="MIW95_001590"
     CDS             complement(17542..19242)
                     /locus_tag="MIW95_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3150921.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(19498..19773)
                     /locus_tag="MIW95_001591"
     CDS             complement(19498..19773)
                     /locus_tag="MIW95_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3150922.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIHAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(19892..20443)
                     /gene="mobB"
                     /locus_tag="MIW95_001592"
     CDS             complement(19892..20443)
                     /gene="mobB"
                     /locus_tag="MIW95_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBY3150923.1"
                     /translation="MKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGYRVGVIKH
                     TCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQLYEDMDLI
                     IIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLNDYKNISKY
                     IESCIKNEILSISNVEIKNILKE"
     gene            complement(20448..21629)
                     /locus_tag="MIW95_001593"
     CDS             complement(20448..21629)
                     /locus_tag="MIW95_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBY3150924.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGEVFNKVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMIILNVKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGILGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGIHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVEIVI
                     R"
     gene            complement(21840..22574)
                     /locus_tag="MIW95_001594"
     CDS             complement(21840..22574)
                     /locus_tag="MIW95_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150925.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSVVIEKSDHEKSAVSLENFR
                     RIDLGEYVGVEESRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            22936..23841
                     /locus_tag="MIW95_001595"
     CDS             22936..23841
                     /locus_tag="MIW95_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY3150926.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKGVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(24001..24843)
                     /locus_tag="MIW95_001596"
     CDS             complement(24001..24843)
                     /locus_tag="MIW95_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBY3150927.1"
                     /translation="MTTSSKKLDTISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSSSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGIV"
     gene            complement(24827..25585)
                     /locus_tag="MIW95_001597"
     CDS             complement(24827..25585)
                     /locus_tag="MIW95_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY3150928.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            complement(25602..26072)
                     /locus_tag="MIW95_001598"
     CDS             complement(25602..26072)
                     /locus_tag="MIW95_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3150929.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDELLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(26072..26815)
                     /locus_tag="MIW95_001599"
     CDS             complement(26072..26815)
                     /locus_tag="MIW95_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBY3150930.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLELGDMAIIGSYF
                     KDNYKDFGNVSVEHVKTFMDEIKKIREEL"
     gene            complement(26819..27232)
                     /locus_tag="MIW95_001600"
     CDS             complement(26819..27232)
                     /locus_tag="MIW95_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBY3150931.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDIECEYIILSDLYGASVCTAMLRLTNLHNVKLFSGMNLNMVLELLTR
                     FSDELTVEDIEQLVEESRRGIQSVTLQIKEEEEVF"
     gene            complement(27258..27842)
                     /locus_tag="MIW95_001601"
                     /pseudo
     CDS             complement(27258..27842)
                     /locus_tag="MIW95_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(28027..28410)
                     /locus_tag="MIW95_001602"
     CDS             complement(28027..28410)
                     /locus_tag="MIW95_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY3150932.1"
                     /translation="MKISKLPEAELKVMRYIWEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLISALH
                     SKDDEVDEDKLIELEEHFKNLKEKE"
     gene            complement(28515..28892)
                     /locus_tag="MIW95_001603"
     CDS             complement(28515..28892)
                     /locus_tag="MIW95_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY3150933.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLIDKGYLARDIIKFQSYYKIIIDNEEYYAFKKKVLKSSKSRKIMRSLT
                     TTHKNVSKEKLDSLEEYYKNLKE"
BASE COUNT         9492 a         5096 c         2874 g        11496 t
ORIGIN      
        1 agtagtattg gataggttct atcctatctc tacatataag tttccctata tgctgactat
       61 tttagctact aactcatgac taaagtcaca agtgtgcgta gctatttttt aatcaaagta
      121 tctagctatc ttaaaggcta agatgataga aggattatac ctaccatttt ctaatgaacc
      181 tatagtttgt cttgatactt gcaaaataga agcaagttct tcttgtttta tgcctctttc
      241 ttttcttatt tcttctaatc tatttttcac atctatcccc ctttaatatc tcaactacgg
      301 aaagtctact ttccataatt atacaactat atttcattta tgtaaagctt gctttccata
      361 tttacaaaaa aataacagac tattttcagc ctgtaatttt aaaatttcat gttgtttatt
      421 aaatttaatg ttattcatta actttaactt ggtttctgaa ttggaatcca aacttctgta
      481 gtataatcag gagcattgat atcgccattg ggataaagtt caatatctgg taagttagca
      541 tacacatacc cagaacttgg taaccactca gtaataattc ttttttgcaa ttcctgtatt
      601 gccagagcat ttggcattgg agaaacacat tcaaatactg cccatgtacc tgatggtatt
      661 aagtattcac gagtatcttc taatacaggc ttatctgttg gtgcagcaat ataataatct
      721 aaacccttac tatttgtaca cacactaact ccaagcaatc cacaaggttc gttatttatt
      781 aactctgaaa gtttatcaat tgttccattt tttatgcatt tagcccataa cttaggaact
      841 ttctcaaagc actcttctac aatcatgttc atactttctt ttataccaac aatacgaaac
      901 cctttctttt ctacaatacg atattgcatt tctacatttc cttttacata aatgcttaat
      961 gtcatcttag gatatgcttt taaaacaaca ccctttgctc tagctgcaga tggagaaatg
     1021 ttatgtatat tttgaaaagc tctattaaaa gatgttggtg aatcgtaacc atacttctca
     1081 gatagttcta aaattttgat gttactgttc tgaagttcaa atgcagcctt agtcatacgt
     1141 cttcgtctaa tatattcaga taatggtact tctgcaatat atgaaaacat tctttgaaaa
     1201 tgaaaggttg aacaacatgc aatttttgct gcttctgcat aatccactcc actttcgaga
     1261 ttttcttcaa tataagtaat agcttgattt aatttattta tccattccat ttgatattac
     1321 ctccaagcaa attatacagt ttaataaaat aatatacctc tctattcatg cacaaaattg
     1381 taagcattta aattcataag tatttttcaa tcaatacaat aaatataaaa attgtattat
     1441 acaataaaaa agttcaataa cattttatta taaaatatta ttgaactttt tataaaaatt
     1501 aatttaagtg tagttaaatt aagtattaaa ctatcttata tattacaata tcctactatc
     1561 ttatagtctt ctggcacttt ataatctttg tctggagtac ctaaattcca ttttaaatca
     1621 aacatagttg cacttattat tgttgcaaaa taaagcataa ctataccaat gtctattttt
     1681 tcttcatata aattagtatg accatctttt ctaacagcta aaactacagt gcctccatca
     1741 actataaatc tccatggttg tctatttaat gtagatggag ctaatcttgc atagtgaaat
     1801 gcatcaagaa gtgctctttc ttctaagatt tctatacttg caacatttcc ccattcgttc
     1861 atataaacaa catcttctac acctagtcta gtagacccct tatctgttac aacttgcata
     1921 tcagattttg aataattttg cccagttttt gtagcattta atactttagc attacttaca
     1981 ttttctccat atccaatagc tataattcct gttacctctt tatctgaatt aattccaagt
     2041 ttttctttta ccttttttcc atcttcaaaa gtaacccaac aagaaccaat tccaagttct
     2101 gttactttaa agattatact ttctcctatg taccctgaat tttcaatgta attatctttt
     2161 atgtcagata atattataat ataacttgga gaatctatca tatttccttt gtaccctgct
     2221 actttatcta aattattaaa aacttcactt ttattcataa ttcttacatc aacatctatt
     2281 tctggaacta gtttcttaca agattctgag tatttcttaa tttcactaag atattcttct
     2341 ttaatatcta tgttcttata ttctctaaca gactttctat ttaatatgat tgttttataa
     2401 tccataattt tatcctcccc aaactagtat tgtcttaatt atatacccat taatatatta
     2461 ttttactcat ttatttaata aataaagatt acaattttgt aattattatt tattaataat
     2521 attccacttt tacattaata atgaccagtt gtgcaaaatc cattgccaac caccaaataa
     2581 aaacatatta taaaagtaat ataaaataac aaacaaatct taacaaaaaa ttaaactttt
     2641 tttacacgaa agaaggcttc ccgttgcctt ctttattttt ataaaaaaat aagctgtttc
     2701 taaaatgatt tcacattttt gaaacagcca agttattcta taatgacaac tataatttga
     2761 ggatgtgagg gagagttgtc attataaatt tagggtaatt atattatgtt ttaagctagt
     2821 tcttcctata tttatataat acattttata tatgaatttt atatgagatt ggtataaatg
     2881 atatatgaaa ttacatatgt ttatattata ttaaatttta ttattacatt ttccacaaca
     2941 atactcatat gtctccacat tatttggaat atttacaaat acaaattctt tacagttctc
     3001 acaatacttt aatatagata tagttccaca agtgtaacat tcaaatatta cacaatcctc
     3061 attttcctct actatttcaa actttttact atgttcacca cactctgggc acttgtgatt
     3121 atttacattc ataaatactc ctccttatta gtatagttac atttcaaaaa cttaattaat
     3181 gtattctata ttacttatta tatattctat aataaatcat attctaatat aataaaacaa
     3241 attaaataaa aaaaaaccat aaattttaaa tttatggatt aattcttgta atgataactc
     3301 tatttgagga tgtgagggag ggttatcatt acaatagggt attatattat ggtttttaag
     3361 ctagtctttc tacacctcat atgatacata tgctatatga attttatatg atttttacat
     3421 aaatatttta tgaaatttaa attttttagt cattttgaaa tttgtaaaaa gttctctaaa
     3481 aatccacagc atcataaaca gccaaaattt aagtaaaaca tcataaaaaa atttgtccaa
     3541 taattttaac aagtcatttc acctcattat aattattgac acaaacttaa ataaacaatc
     3601 tataaatctt atataaatct taaactttac ccttattgat acacaatttc tagtttgtca
     3661 attttatttt gcatctctaa gaaaatttct tcaacagaat tgttaaatat attgcctaaa
     3721 tagactcttt tatctttttg aaatgaataa gattttacac taccatcaga agaaatatat
     3781 aaatgactcc actctgctcc atattttgaa aacctctcct ttgtatcttt aaacctcata
     3841 tcttctattt ttattgcttt ttctctgtct cctcttacta cagtgtgacc cctttttatt
     3901 aaactggtta tttttatatc ttcttttaat ttaatacgtg tatcatcatt ttttgaatat
     3961 actttccata tcactttatg acgataatta tctaattctt ttattaagct tctatcaata
     4021 gtaaagctat ctttagttac cacaattaaa tcaaaatggt ctaacatata ttgaaatatt
     4081 tctctcatac ttttattttt taaaatatcc tcaccaataa cctctataaa tctacagcct
     4141 ttctcataca tgcagttcaa taaacttgtg atttcagaaa aataatcatt tgtattatta
     4201 tctccatcta tttctatagt aaagtacaaa ggagtaatgt atgatttatt tcctgttatc
     4261 tcaaatacac ttgatagtgt ctgtatatca tcttcaaacg ttaagtatac attctcataa
     4321 aacataaaaa attccataac tttttctttc atattatgga tatttctata tctattggat
     4381 aatatattta ttatatcgtc agtagtattt ttcccatcac attcagccag tataacaatt
     4441 tgcttccaac ttaagctatc cacatttccg atactttttt tatatccatc ttcttcatca
     4501 taaataggac caattataat gttttctttt ggccacagat gaaccctagt tccatctttt
     4561 aatttcggat atttattttt tacagactca taaaatctac ttgtatcacc catgattctt
     4621 tactcccccc catagtttaa atattctatg ctaaaaatag cactttattt ttattaattc
     4681 aacaattatt caaaattggt cattatatat gtttaaatgg atttaacatc aaccttattt
     4741 atatgttatt gatgtttata tatgttatat aaaatcttat ttctcatact tcaatgtaat
     4801 cttaactatc tctggtttac aattaagcct aattaaaact tttgagcctc ctaagccttt
     4861 atttaaaatc atagtgctat tatctttttc atattttccc cagtcatact taggaaagaa
     4921 ctctctattt ggagataata cacctccaac aaaaggtatt cttattatac ctccatgaac
     4981 atgtcctgct aaaaccaaat ctgctcccca ttttgcatat ttttcaaaga aaaatggtgt
     5041 atgtgctaaa agaatgttat attcattttc attaacttta cctagattgt cttctacaaa
     5101 attgtcactt agttctaatt ttttgttatt agcaaataaa tatggatagc attccaatgg
     5161 tataattaaa ccatataaat taagatggct attacctcta tatattctta cagattcatt
     5221 ttccatattt attattttct ttgaatatag ttccttaaaa taatccttat atagatgttt
     5281 atactttttt attaaagatt tttgttcatg atttccaatt atatgatata cttcatattt
     5341 ttcaaccaaa ttatctatta aatctaatgc aacttgaaaa tttttatttt ctccatctac
     5401 taaatctcct gttataaata taacatctgg gtttaatgat tcaattttat ctatcaatac
     5461 cttattattt ttatcaaaca ccttattatg taaatcactc acttgaacta ttttaaagtt
     5521 atcaaattcc tttggaattt ttttgttgaa aatagtatat tctgtcgttt ctatatgctg
     5581 agtttcatac ttatacagaa tatacaataa tattaatact aatactgtca ttgaaacctt
     5641 tataatcata ttctcttcct ctttattcta ttttgttaat attattataa tgcttttatg
     5701 ttttttttaa aacaataaca tcatcacata catataaatc ttttcgacat tataaaaata
     5761 agttatcctt ttctaaaatt ttcatttatt ttagaaaagg ataactataa aattatctat
     5821 aaacctctat attgtgaaat atagtttttc ctttatttaa atttttatat atatgctcta
     5881 aagttccatc aaacctaata gaatgtccag actctaaaat ataagttttc ccatccacat
     5941 taaggtcaag tttaccttct attacgagta catactcttc taaaacataa ctatgtgtag
     6001 atgaaacatg agtacagttt tcctcaagct caattataaa aagttcaaac cttctttgag
     6061 ggctaaaagg gtatattgga tataacttca tgttcccttt ttgctcaaca ataggctcta
     6121 cttcttcttt ctttataacc tcataggtat ttgtattttc atttaatagt gtagtaaaat
     6181 ttattttaaa tccagatgaa attttccata aagtagatac tgtcggactt gattcacctc
     6241 tctcaatctg tccaagcatt gctttactga cacctgtcat ctttgaagct ttgtctaaac
     6301 ttatgcccat ttcttgtctc aataacctta aattttctcc cacattagga aatttattat
     6361 ccatgttaaa atcctcctga tagctatttg tattctatga tttttactcc tgtaagttct
     6421 actatctctt ttaattttaa catatgattg ttacaaattt catcaaattc atatatataa
     6481 tcttcaagcc ctaatgcctt atatttatcg attccaaatt tatgatatgg aagtaactcc
     6541 atcttgcctc ctggaacatt ttgtttgaca aataatgcag tatttcttat attttcttca
     6601 ctatcattta ctccataaat aattggcact cttataacca tagatttatt caactttgaa
     6661 agcctacata tattatctaa tataatttta ttacttactc ctgtatattc tttatgaata
     6721 ttatcatcca tagattttat atctacaaat atgtggtcta ttttctcaaa tacatcattt
     6781 accttattcc aatcaaaata tccacaagtt tcaattgcaa tatttatgcc tttatcataa
     6841 aaaatatcaa ctaattctct taaaaaatca atctgaagtg taggttcacc tccagaaaaa
     6901 gttatccctc cattagattc aaagaaaaat ataaaatcct tttcaacttc ctctactatt
     6961 tcttcaacac tcattttttc ggtcattata caaatggcat ctttaagaca taccttgata
     7021 cactctccac ataaatcaca tttattattt atttgcgttc tatcaaatag tgaacttata
     7081 ttttgaggac aaacatctat acatctatta caggatacac atttatcttt cataacacct
     7141 aattttacta tgttactcca ggaatctgga ttagaacacc acttacattt taatttacat
     7201 ccttctaaaa atatagtagt ccttattcca tctccatcat ttactgaaaa atgttgtact
     7261 tttacaactc tacctttttt actcataatc tacctcataa ttaaaaattg ctatgtgaat
     7321 ttctagctat tatagcatct tgcatttctc ttgataaatt aacaaattga gtactatatc
     7381 cagcaactct cactaataaa tctttatatt cttctggatt ttcttgagct tttaatagag
     7441 tatcatttga tatcgtatta aattgtacat ggaatcctcc taaagcaaaa tatgatttaa
     7501 tcatagatgt tagatttcta agtcctcttt cagtttccac caaatcttga ttcagtctta
     7561 aatttagaag tgttccacca gtatgaacat catgatttat ttttgatgca gatttcatgg
     7621 ctgctagtgg acttgttgta tcagtaccaa caaacggaga aactccctct gtcaaaggtt
     7681 ttgtcgcttt tcttccacaa ggagttgcac ctacgatttt tccagttggt acatagtttg
     7741 atatacccat aaatgcacta ttaaatttag agccaaaaat atccttgtat tttctagttt
     7801 cagtataata aaaatcagaa acttcaattg ctaatgaatc tacataatca ttatcatttc
     7861 catacttagg aacatccaac gcaagttttc ttaattcttc ataaccttcc caatcattat
     7921 ttagtgcttt tgttaattct tctagcgtag tcaccttatc ctcaaatact aattttttta
     7981 tagctgccaa tgaatttgaa actacaccta accctatacc agttaataca ggacctatat
     8041 tatattttgc tcctccttta cttaaatcaa ctcccttatc caaacatcca tctacacaag
     8101 tagataaaaa tggtcttggt accatttctt tatgaatttg ttgagcaaca gtagttccta
     8161 taacagaact ttttacaaga tatgctaatt gctttaaaaa ctcatcctta agttcttcaa
     8221 aagaagtaaa ttctgtgata ttttttgtat ctaaccccat tttttctcca gttaatctac
     8281 tcttaccttc attcatagca tattctaaag ctgctgcaaa attgatattt acggctgaag
     8341 tccactgtcc tgtttttcta aaatgaggaa ctacacaacc acagttactc caatctctag
     8401 catcttctgc atcatatcca tcttgtaata acatttgtgc ccctgcacta tcgctgtgta
     8461 tagcagggaa accagttcct tgttttacca atttagcaac tgccattacg aaattatctg
     8521 gtgctccttg actcactcta acacttaatc ctggctggtg agtttttaca ctttctgtag
     8581 cctttaaaca catatatgag atatcattcg ttccatcagt accatttctc tttttaccac
     8641 caacagtaag attttggaat tgattatatc ctgcaaagta atttgctgta tttgctgata
     8701 tagtccatac ccattctgat aacttaatcc aaagtgcttc tatcaattct tgagcttctg
     8761 attcagttat tttaccttct ctagcatcat tttcataata tggatacata tattggtcaa
     8821 atctacctaa atttaatgcc aatggatttt ctgatagaat accacctaat tgaacaaacc
     8881 atacaaattg tattgcttca taaaaattag ttggagggtt tgctggtaca ttcatacata
     8941 tctctgaaat ttttataagt tctaactttc tcttttcatc tgtttcttgc ttactcatct
     9001 cttttgcaag atttgaatat ctttgtgcca atgtcataat accctctgca actatagaaa
     9061 cagatttgta aaaatctatc ttttcaatat tttctgaaac tgatatatct aattctttaa
     9121 tttttttatc tgcatcttcc ttaatccctt tatatccttt tacaaacaat atatcttgat
     9181 aatctggtgt tacttcacca actgcctgtc tccacttaga atcattatct attattcctg
     9241 tatctactaa tatcttagct gtatcttctg ggattcttgc taagaaaact tcttccaaag
     9301 attttccctt ccaatatgga aatatttctt ttcttataaa ttctatttgt tcttttgata
     9361 ttacatatgg gtcttgagtt cttttatcaa aagtatccat ttctttgtct acccattgcc
     9421 aagaaaactc tggtgtcaat atccctgttc ttctaaattt tcctgctgta cctactatta
     9481 attcattttc aaagattttt ataggaagtt ctttacatgt ctccaaaaaa gctttagctc
     9541 ttcttataca gatagcttct ccctcagttt ttttatgtga ttctgtaaaa attctagccc
     9601 tttcacaaca tatcataggt ttagaattaa tatattcttg tctaaatgct tctattctat
     9661 tagtttgact catttaaaat ccccctttgg ataatatata ctacattttt gtatgttata
     9721 acatacttta attatattat actgtccttt agcataaaat ggaagcaaaa taactttttt
     9781 tttcatctac cttgaacacc tatatattaa tacttttttt gttaaattta taaaatctaa
     9841 aaaaattttc tatgatatat tttctatatt gttatagtta tttctaatat actaatgatt
     9901 tgaaatatca aatttgtata ctagacttag ttttaaaacc ttagttgatt gtttaacttt
     9961 gttaacttca acatgtatat ccttcatata aggaagctcc acaacaccta ctcccttatt
    10021 aaattcttct tcaaccatag aaaaaatttc tttctcagtt aagttcgtat tagtcaatga
    10081 tttaaataaa ttgtatataa atttaatata tttatcactc tcagataact tatcatcttc
    10141 tggatactct ttagcacaga gaatatctat tgttactttt ccttctttat cgtcttcaga
    10201 catagaatag aataaacttc gaatattttt ttcaggtggt ttttcagcac catttataga
    10261 tactacaaag ctaccatctg attttgagtc agtaatacta taaccactgt ctattatagc
    10321 tttcgaaaat ctttgagata aatctttttt tacttcttct ctacctttcg catcattaat
    10381 cttttgtgag taattattaa atagctcgat agattcatct tttttatctt caacttgttc
    10441 tttcaatgaa tctttactaa tttgttctga gctacttaag ttcttatcag atacaggaga
    10501 catacatcct gccatcaatg ctaagctaag atttaaagcc acaattattg ccactctatt
    10561 tatattcctt tttactttca tacacaatcc tcccatcaca ctaaccgctc taaagtatta
    10621 ttcattgcat tagaatatat ttatattttc tttatattaa agatttatta ttcaatacat
    10681 atcaccttcc tatccaattc ttataactta ctattagtaa aattataagc tccttaatac
    10741 agaaataagg atgttatctt ttgttattaa aagataacat ccttattttt ttgtatcaat
    10801 acatatgtcc caactggtaa cttaatcgcc ccaactgtca gtatcttgct ccaatggtat
    10861 atatatattt agtataaatt tatcgatact attttcaaat aataattctc cattatattt
    10921 ttgtaaagaa gatttaatac tctgaatacc tattccatgt acgaacttat ccattttgtt
    10981 tgttaacaat atatttttat taaaagtaag ctgatttagt ttactatttt cacatttaag
    11041 tacaaaaaat gactttgtta tagttccttt aattctaata tatttattaa tattttcatc
    11101 cactatttta tcacatgctt ctattgcatt atccaaaata ttagaaaata tgcttgacac
    11161 atctataggt tttacaaaat ttacttttga aaaatttata gagcaagtaa atttaattcc
    11221 tttttttaaa catatttcac tcttttcgtt gattataatg tctaatatca tatttcctgt
    11281 attatagata tatttaaatg tttttatttc atcttttaaa ttgttaacat attcattaat
    11341 ttctttgcta ttgtttctaa ggttgtctat acaataaatg tggttattta tatcgtgata
    11401 tagtttctta actcgcatat gagattcttg aatacttaaa taatgcgcat attgcatatc
    11461 taacttattt tttattaatt cagcttgaga ttttaatcta ttctctttta ttatttcttt
    11521 aaacaataat ataagaaata tactactagc tcctaccaac tttggattaa caaaattatc
    11581 aaaatagtat ccttctgaat gtaatgaccc aaaccgttct aattttttta atacataatt
    11641 tacaagcata ttcattgtat tagctaataa ggcaaataat aaatatacat aatttctctt
    11701 atcttggcta attaatttta tagacttcct aaaaaagcta aatacaagat atataagaat
    11761 aattattaaa ttactatata tctgaatttt tatagattct aagtgaggta ctaataagga
    11821 ttctttagat gaaataacat tacctgtaat tgcaaaaaat agtattctat atataacagc
    11881 atccaaacat ttataatata cactaaatag ataaaagaaa atggaatact taatataatc
    11941 catcttataa aaataattat agtatcctcc ccaagcaata aatgctaata tataaaataa
    12001 atagtttata ttactaaagt aacttcctcc attaaataat ctacttatct gaattataat
    12061 ataaataaga aacaagacaa tatagaatac tattttttca ttttttttac tatccttttt
    12121 ataaattaaa attccgttca ttatagaaaa ctgtataaat atagataaaa ctactaatag
    12181 ctcattcata aaatttagca tattgtatca ccaagaaatt ttaaaaattt ctccttaact
    12241 tctttatatc tatatctact aataggtacc tcttctccac tttccagtat tgcagcatta
    12301 ggctttatat tttcaacata tcttaaattt actataaaac ttttatggca tcttataaat
    12361 ttatatggat ttaattcttt ttctatttta cctaaactat attttatatc aaaattcttg
    12421 tttattgtgt gtattagcat atcttttttt tgaacttcta tatattttat ttcatttaaa
    12481 taaatcttat atgtattaga cttatttttt attgtaatat gactttcctt atttatttca
    12541 atatctttga tacaagttag cacatgtttt tttagttctt ccaactctat tggctttagt
    12601 aaatacctat aagctcttac ctcataacct tcttgcacat aatctattaa tgaagttaca
    12661 aatattattt ccatttcatc gtctacttct ctaatctttc ttgcagtatc cattccattt
    12721 aatttttcca tttttatatc aagaagtaat atatctatat cttttaaatt actttccagt
    12781 aactcttctc cagatatata cttttgtatc tcatattcta tacttatatc ttttaatata
    12841 ttctcaaggt aatcttctat taaaagttgt gtttcttttt catcttcaca aactgctatc
    12901 ttaatcataa aatgcctcct tacataattt ctagttaata caattaaaat atttataata
    12961 tatttaaaga taattttgta tatctgatac tttaggataa catttttaaa ttatttttat
    13021 caatactcaa tccaatacaa ctattatttg atatttcaat ttaaagacag aaattactat
    13081 aattaaaaac agtactttac taacaaaaga agatgtctta aaatattaaa aaaataattt
    13141 taggacacct tcttttattt atttaaactt tttaagcctt aaataataca ttaatctcct
    13201 atataattgt actgcatata aaatcaacaa tataatctgt agtacaatag cagttacaga
    13261 tgctccataa ccactatata aatttcttat ttcaatattc aataaatata taaatattac
    13321 tactaacata aagaatataa acatattcat aaataaaatt gactttactt tgtttttttt
    13381 atcagaatca ctatataatt taggaggtat gtctttgtat tctttcgacc atatatacca
    13441 tgcagatgta cccataaaac taccaatact cctaaataca agattccagc catttgattt
    13501 atgttcatca aaataattat tctctacatc atagtagtaa tcaacagtgt attttatttt
    13561 tcttggactt cccttatgaa aataaaatat tcctccaaat ttatttatct tatataaatt
    13621 atatccttta gcttcaatat cttctaacca tttctctatc ttatcaggtg aactatacca
    13681 gtcaaatttg aattttattt tcttttccat agcactcatt ctccaaaatg atatttttaa
    13741 atagcttaaa aatctattta aataattata tcattttgat actaatttta ggttacaatc
    13801 atatatttaa aaagctattt aaatagtaat aaactaatag ccataacacc cattcctgca
    13861 atcaatccat aaatagataa atgatgttcc ccgtactctc tagctgatgg caataattca
    13921 tctagtgata tgaataccat aattcctcct acaattgcaa atacaattcc aagagttata
    13981 tcatttaaaa aatttctaag aagtacatat ccaataatcg ctcctaaagg ctcagacata
    14041 ccagataaaa aggaataata aaaagctttc tttttatctc ctgtggcata ataaactggt
    14101 acagatacag atataccttc tggtatattg tgtattgcaa ttgcaattgc tatcgggata
    14161 gctattgtaa catcatcaag agctgatata aatgtagcta atccttcagg aaaattgtga
    14221 attgctatag ccattgctgt aaatatacct gttctcaaaa gagacttatt ttttttgata
    14281 ttcttttctt ctatattctc cattttcttt atttcatgtg gattttcttc ttgtggaatt
    14341 aacttatcaa taacagcaat gatagccata cctataaaaa aagctattac agttgaccaa
    14401 cttcctaatt tctctcccat agattttgtc aaagcatctt gtgctttagg aaaaatttct
    14461 atcatagaaa cataaatcat aacaccagca gaaaatccta agcttataga aagaaactta
    14521 gtatttgttt ttttagcaaa aaatgctata caacttccta taccagtaga taaaccagcc
    14581 aataatgtta tcaaaaatgc aaatattaca ttattcaaat aaatcacctt ctttttaatt
    14641 gatattgatt atcattttct ttatgctatc atatatattt tatatagtca atatttataa
    14701 aaaatataca caataaaaaa gcagagatat aaacttagtc tctgcttttt ctttatagta
    14761 tattaaaatt atttatttaa gaaatccact gcatattgca catataaagc agttccaatt
    14821 tctagagcat cttcgtctat attaaagcat ccatgatgat gaggatagca agcacctttt
    14881 gactcatttc tagctcctac aaatgctagt gcaccaggtg ctatattcat aaactcagct
    14941 aaatcttctg ctcctgttac tttttcaaat agtgttatac agtcttctcc aaatagctta
    15001 attgctgcat ctgtagctat ctttgaacat tctttgtcat ttataactgc tggtgttaag
    15061 tacccatact ctaattcagc atctgctcta tatgcttctg ctgtagattt tgctatacgt
    15121 tctaaaattc caggaatttg ctttctaagc tctggattaa atagccttat tgtaccttct
    15181 aagacagctt cactcgcaat tacgttaaat ctagttccag aatttaaaac tccaacactc
    15241 acaactaatg gttccagagg acttacttct ctactaacca tagattgtag attcataact
    15301 attgctgaac ttgcaagcac tgcatcaaca ccttgatgag gaagtgaacc atgaccacct
    15361 ctacctttta cagtaatttt aaagaaatcg gcagaagcca ttcttggtcc ttcttctacc
    15421 gatatagtcc cagattctac atcagtccaa agatgtattc caaacacact atctacacct
    15481 tccatagcac catcttgaat catagctctt gcacctttac caacttcttc tcctggctga
    15541 aaaaataatt ttacagttcc attgatacta tccttaatat catttaacac ttttgctgcc
    15601 cctagaagca ttgatgtgtg accatcatga ccacaagcat gcataagtcc ttcattttta
    15661 gatttgtatt ctacatcagt gcattcaaca acttgtagag catccatatc acccctaagt
    15721 gctacagtct ttcctggatt agctcctttt atagttgcaa ttacacctgt acctccagct
    15781 gatacatatg gtatctctat tttatcaagt tcttctttaa ctctctttga tgttctaact
    15841 tcttccatac ttttctcagg attttcatga aattcccttc taagcttaat tacatagtct
    15901 ttataactct ttgttatttc tttaatatcc attccatacc tccaagtagt acacattctt
    15961 cttatttaaa atacttttta tcacttaaat actatattag atttacaaag atacctgcta
    16021 ttactacaga agctattgta actgtagtaa atcctccaac aagcatcttt ggaagcatct
    16081 tatccattaa aaattctttt tcttctggtg tttcagccaa agcctttact gcctcttcag
    16141 ttaatatata gtttggtgga aaaccataaa gtgatgtaag cgaaactgaa aaagccattt
    16201 ctttactttc acctaaaagc ttacctacta taatagagaa tattcccata cctattacac
    16261 caaatattat gattcctaca agaggaactg ccatttcaac aagcattgct ggtgtagctt
    16321 ttgctaatcc tgcaaatata aaagccatca atgatgtcat taaccatcca aatgaaccag
    16381 atttattaag tggttttcta tcaactattc caagttctga tgctattaca ccaaatatta
    16441 aacatataac atatcttgat acaacttcat ttgtaagatt tgtaaattgt actgctaacc
    16501 atgctacaaa tcctaatcta agtattatca tatatgtcgt atcatatttt tctggtaagt
    16561 ctggaattat cttaaatctg cttttttgtt caggtaaatc cttaactttt tcttcatctg
    16621 tatttacagt tacttttcca cttctaaagt ctgataaaag tctttttcct tcttttttaa
    16681 gcattaatgc tgtaatagga taacccgcaa atccttgtgc aacatacatc aaaattgcaa
    16741 gtactgataa acttgcaaga ccttttgctg tagcagcatc tgccatcatt atagatgcaa
    16801 ctagccctcc agtaagagga ggtgtagcta ttactgctgt atcccatcca aatactattg
    16861 tccctaatgc caaagcacct acacatattc ctactattcc tgccaaggct attactattg
    16921 ttttccattg acctgctaat tccttaatac tcatcatagt tcccatatga gttattaaca
    16981 aaagcatacc taattgagct aatggtgttc ctaaagcggc taagtccaca atattttctg
    17041 ggaaaaaagt ccagaatcct actataaata taattgctga tacaaaaacc gacggaacaa
    17101 aagctttagt ttttgctgaa actatatctc ctatcgtcag tgatattaaa actagacaaa
    17161 aagctgttat tgcctccata caaaatcccc cctcataata aatgtatttt tataaaatgt
    17221 atgtatagtt atccattatt ttactacttt tgaaaaacat ttcaagtcaa aattcgattt
    17281 ttttcataaa attcgataaa ttccataaat taaagttagt tttctaaatt attctttttg
    17341 taaaaacgtt atcctgacca ttggctattt aactctattc ttacaaagag aattaaatgt
    17401 aatttgcaaa aaataaaaca taggatacat tttattcata aaacatctca taaatataaa
    17461 atgaattgtc tttatgaatc ttaatatatc ctatgtttgt aagtttaatg cgtatgttag
    17521 ctatggtgta aaatttgtat actagttatt gtgtaaccta ctatatactc catttttgtt
    17581 tactaactct tcatgagtac ctctttcttc aattccttta tccgtcaata ctatgatttc
    17641 atcagcattt tttatagtag tcaatctatg tgcaacaact aaatttgttc tatctttact
    17701 taattcctct aaagactctt gaatttctct ttctgttaca ttgtctaaag cagatgtagc
    17761 ctcatccaat attattattg gaggattctt taaaaagatt cttgaaattg aaattctttg
    17821 tttttgccca cctgataatt ttacgcctct ttctcctata taagtatcat atccatctgg
    17881 caaagtctct acaaagtcat gtattctagc ttttttagca gcagctatca tttcttcatc
    17941 tgtagcatta gggttcccac aaagaatatt atctcttata gttcctgtaa ataaaaatac
    18001 atcttgttga actataccta tattttttct caatgatttt aaactaacat cttttatact
    18061 ctttccatca atttttatat cacccttatc aaattcataa aatcttggca atagattaca
    18121 taatgtagtc tttcctcctc cagaaggtcc aactaatgct atagtcttac cagcttttat
    18181 agataaactt aaattatcca aaacttgagt cttatcttca tatgtgaaag atatattttc
    18241 tatctcaatg tcacctttta cattttcaag ttcaatagga tttttagctt ctttttgatg
    18301 gtcttgttcc attatctcta tgaatctttt aaaacctgtc ataccatttt gatattgctc
    18361 tgtaaaattt attaattttt tcataggttg tataaacatt tttacatata gtatataagc
    18421 agcaaaatca cccaaagtaa tttttcctat ataagtaaaa taaccaccag ctattacaac
    18481 tacccactct aagatatcca tgtataaatt cataccagag taatattctg ccattacctt
    18541 ataagaatct tgtcttgctc ttctaaataa tttattgctt cttacaaact tatttaactc
    18601 ttctttctca gtacaaaaag acttagttac tttcatacct gctatactat tttctaaagt
    18661 agcatttaca tcacctatag taactctact cttcataaaa gcaatattca tcttatcttt
    18721 tttagctatt gcaaaccaaa ttataaacgg taatatagca aatattataa gcgtaagagg
    18781 aatatttata tctataagta taaagaatga accaaccaac attactatag aaatgaataa
    18841 atcttcagga ccatgatgtg caagttctgt tatatccatt aaatcattta ctattctaga
    18901 catagttact ccagatttgt tattatcaaa atacgtattt ggaagtcttt gtaagtgtgt
    18961 aaatacctca tttctcatat cagcttgcat atctacacca ataacatgtc cccaatactg
    19021 cataaaatag ttaagccctg cttttgctat aaatataagt ataagtgcta ctgcaaaaac
    19081 tactaacatt cttaagtttt tatttggaac aacgtcatcc attatatttc tagttatcat
    19141 aggataaact aaatcacata aggaaaatag aaaagctgct ataaggtcta gtgtaaaaat
    19201 tttcttatat ggcttataat atttgacaaa ttgttttatc ataagtatta aatccctcct
    19261 ttattagtac ttctatatag tacttctaaa aaatacccat aaattagtat aacataacaa
    19321 taactttaaa tcttttataa acttacaaag taataacaat ctttttcata aaattgaaga
    19381 tttatatcat atagttgtat acttgaaaca gcataaaaag agctatatct ctatatttaa
    19441 aattttataa agatacaact ctatatttaa tactaaattt tttttataaa cacaaaacta
    19501 cttcaatgca tttaatatct ttttatctaa tgctgttgta tttatacctg ttatatatcc
    19561 tgttgccttt attgtaggta tatcataagt tgttggtagt atagtagtag atacgattaa
    19621 atcagcacct tcttgttttg ttgaacattc agcaatttta cattgctcaa cttctgcgtg
    19681 tatattattt tctttaacca atctttctac tctgtcacaa actattgttg atgttgcgat
    19741 tcctgctcca catgctacta atattttttt catatattgt cctcctacaa tttctttaat
    19801 attttataac tcaaaataaa attgataaat ttcttatatc tttatttata taaataatta
    19861 tatcatttta tataagaaga acatcttaaa attattcttt taatatgttt tttatttcaa
    19921 cattgcttat acttaaaatt tcatttttta tacaagactc tatgtacttt gatatatttt
    19981 tataatcatt taaattaaac tgttcaatat tcaatttata ttctatatca ctgacaatac
    20041 ctatcaaatc agacttatta gataaaatat cctcatactt accttttcta gttacttcca
    20101 atcttttaaa tctataattt ttatatcctt ctatgataat caagtccata tcttcatata
    20161 attgaatcat tttatataat ggattcttct cttttagttc ttcaatatat gctactcgtt
    20221 tatctgatat tatacagact tttctcgccc cagattttct atgtttataa gtatcttttc
    20281 cttcctcatc catatcaaaa tcatggcagg tatgtttaat aactccaaca cgataccctt
    20341 ttttatttaa ttcttttatt atgccttcaa tcaacgtagt tttacctact cctgaatatg
    20401 agacaactga caatatatta tacataaaat tgtgtccttt cataaaacta tctaataaca
    20461 atctccacta attgattttc ttttattgtc tcattctttt taatcttaat catacaatta
    20521 cttcttagag ttgcagataa tctagctttt gcatctttct tatcatttag ctctacatac
    20581 aatttacctt cacttgtata tagttttcca cttacaaatc tatctttatt tgaaagttta
    20641 ttaaattctc ctttaaatat agcttttata tgatgtattc ccttcttata tgttaaatac
    20701 tcaataacag gaactatcac attatcaaat accactattg ctgcatgtgg attaccagaa
    20761 agaccaaata atagcttttc tcccaatata cctacataac aagctccacc aggtttaatg
    20821 tttatcttcc aaaataattc tgttgcccct atttttttga atacatctgg aattaaatct
    20881 ttatctccaa cagaaacacc acctgttgta attaaaaaat ctacactctg taacatattc
    20941 tttacctttt tttctaactc caatatatca tcagaacaat tgcatattgt atctgcctgt
    21001 attccaagtt ttaataatct tgaatataag gtatatctat tactatcgta tattttacca
    21061 ttctctaaag tctcatttat atcttgtagt tctgtacctg tattcaaaat tccaactttt
    21121 aaatcatcat aaacagttac tttggatatt ccaagacttg ctaatactcc aataccatca
    21181 tatcctatat tctgattttt attaataatc aattgcccct tttttatatc ctcaccctta
    21241 aatatgtaat tatcgtattc ctttaatttt acatttaaaa ttatcatatc acctataacc
    21301 gttacatctt ctttttttat tacacagttt gcaccctgtg gtatctttcc accagtcatt
    21361 atcttcactg cttcattttt atttaatacc ttattaaata cttctcctgc atacaaagtc
    21421 cctataacct ttaaatttct attttcagaa tttgcattat atgcataacc atcatagggt
    21481 gacctatcaa atggaggact atcaattgtt gaatatatat cttctgcaac tactttattt
    21541 actgcatcta taagatttac tatcttgacc ctgtttattg gtaaaacatt tttttcaata
    21601 atttttatgg catcttctaa ttctatcatt ttaaatactc cccaatatat tttttattta
    21661 agatatttct atgtaatata ttatagatta taaaagttgg aaatactttt atcttatatt
    21721 taattataac taatagttat ctataagtgc agttcaaatt ttataaatct aataaaaaaa
    21781 ctgttcccag ctagaaattt ccttctatct tgaaacagca tcatatgctt tagaatattt
    21841 tattctaata aagttccttt tattattttt acaattttat cactttcaat agtttttata
    21901 gattttattt tgttatccac aatacttatt gtaagtacct ctttttttaa tattttgtta
    21961 tcttctttca atctataact cttaactact ttactacctt catgattata tgctgttaaa
    22021 tcataattcg catctcctac aatttcattt attctagact cttcaacacc aacatactct
    22081 cccaggtcaa tacgtctaaa attttctagt gatacagccg atttttcatg gtcacttttt
    22141 tcaatcacaa cactagattc agcatcaatg actgatatac cttgtgatga gaaagaattg
    22201 gtttcaacat tgacaacttt attttcgtta atatcaacat ataaagcact tccatctagt
    22261 tcatcatttc cagtatattt tggataaact aatgtcatat aacttgatgt atttatatct
    22321 tgcattgtat tcgattcttt taaaacttta ggttttatat aataaaatgt attataaggt
    22381 atacccatat tctttataat gtacttataa tctttattca taatttcatt aacatcattt
    22441 tgaacttttg tcatagtttt ttgctgtgtt gctttacttg ctacttgatt tttctcttta
    22501 gtattttctt tatgacatcc aacaagtata atacctaaaa tagttacaca aactaatgac
    22561 ctttttaact tcataaatca atcctccttt cttaaatttt atagtattta tctatatctc
    22621 aatttacagt tatctctttt ttcaataatt atatttatta tattataaaa taattatata
    22681 atatttattt gaatttaata agattaagaa gctaattgta atttttctgt aataaaaaac
    22741 actttaaagt tgaattttga aaattcaatt tacgatattt ttattgtaag taccttacaa
    22801 tattatacca aattattcct aaacaatcaa aaactccatg aatatttaaa ggttttctat
    22861 tttaaaaatc acctttgact attaatttat caaatatgtt ataatagttt tagataattt
    22921 aaggaggatt aaataatgga ttttaaacaa cttgaagtat ttgttgctgt tgcaaaacat
    22981 cagagttttt caaaggccgc aagagaactt tttttaactc aacctactgt aagtgcacac
    23041 atacagaatt tagagagaga gttggaaact gttcttataa atagaagcaa caaagttatt
    23101 acactcacaa aatctggtga aattctttat gaacatgcta tatatatatt gaataattgc
    23161 aaacgtgcaa tttatgatat aaaagaatat tcaggaaaaa tagaaggtat tatagatata
    23221 gcatgtagtt ccatacctga aacttatatt ttacctgatt ttatgaagag tttttctatg
    23281 agttatccag atgtaaaatt ttccataagc cattatgatt ctcaatatgc tatatcagaa
    23341 attttaaatg aacgaataag ctttggatta gttggttcta aaatcaataa tcctcaaata
    23401 gagtacctag acttattaga tgatgaactt gttcttatca ctccttcaga ttttaaaatt
    23461 gataataaaa ataattgtat cgatatagga gaattggctt atctaaattt tattatgaga
    23521 aaagaagggt caggtacaag aaacttaata ctaaacacac ttagtaaaaa taactttcca
    23581 gtaagtaaat taaatgtaat agctcatgta gaatcaaatg aggctattaa ggaaatggtt
    23641 agattaggtc ttggagtctc ctttatatcg tacatttcag ctattgatta tctcaatgca
    23701 ggtaaaataa aatgttataa aataaagggt gttgatttta ctagaaaatt tttctttatt
    23761 tactctaaaa agaaaacttt ttccccatta gaagataaat ttctaaacag attatgtgag
    23821 tatttcgaaa ttataatata ataactttaa taaagtagtg cttaattttg ggtttataac
    23881 taattaataa gctaattata actatcacat aaatgcttat aaattattat gaaccatctt
    23941 tcttttttat ttaatttagt caaaaaatcc ctcgttcaaa tgaacttggg atttttttat
    24001 ttatactatt ccaatagcag ctaatactat acaaataatc ataactgaaa ttaatatcgt
    24061 agttgtcttt accttttttc ctaataacca atacataata ccgaatatca tagctggaag
    24121 catacatggc attatttggt ctaagtaagt ttgaataggc tctgcccatt ctccactacc
    24181 tacattaaca ctaagtttta acattacatt agatgcagtc atacctccaa ttaccataag
    24241 ccctaacatt gaagctgctt ccataacttt tgaaactata cttgaactac tcacatcttt
    24301 aaagaattga gttccatact taaatccttt atctagacat atatagcgta taataaaatg
    24361 aggaacattt attattaaaa ggaataaaat cggtcccatt atgttaccct gagaagctaa
    24421 agctatacca actccagctg ctataagctt taatgttccc cagaaaaatg aatctcctat
    24481 cccagaaact ggacccatta aacttgattt aacagcattt atactattag catcaaattc
    24541 tttattcttt gcattttctt cttccatagc tccagatata cctaccaaaa gagtacttat
    24601 atgaggagta actgacataa attctaaatg tctttttaaa gccttagatt tcttttcctt
    24661 atcatcttta tacaatctac ggattattgg tgccatcatg tatgcatatg ctatattttg
    24721 catacgttcg tagttccaag atgaatctaa tgtcaatgac cttctaaagc atttccaaaa
    24781 gtctttcctc gttattttac tatcgggtga aattgtatct aacttcttag aactcgttgt
    24841 catctactat tacctccttc tctcctacaa agttaacata tattaaagct attgctaatc
    24901 caaacaatgc aactcctgtt actgttatgt ttaggtaagc aaccaatata aatcctaata
    24961 ataaaaatgc tactgttttc ttattaagca tcatcctagc taacatggca aaacctattg
    25021 ctggaatcac gcctgctgca acatccattc catgagttac atatgatggt ataaatgaaa
    25081 gtattttttc tattactgga acacctaatg aaaatcctat acctactatt attgctaata
    25141 ttaatttatt tcctatacca aataataatt gcacacgttc tacgccttca atattcccct
    25201 cttctgcata acggtcagca atatgagctc cccatgtaag tactactgtc attaagaagt
    25261 tatctataag caatactaga gttgctatag gaactgcaag tacaactgct gctgatacat
    25321 cttgacccgt taatattaca aacgctgtgg caataataga accagacaca aaatcaggag
    25381 gattacttgc cccaacagga aatgcaccca taaatactaa ttctaatgtt gaacctatta
    25441 ctatacctgt atgaagatta cctagtgcta aaccaacaag tggtgcaaca actataggtc
    25501 tattatgcat tgtagaacct acaaaactat gcatatatgc aaagaatgca attagagtta
    25561 tcaaaataac ttgtgtaaat tccattttca tccccctaaa attatattaa tttcattaca
    25621 tttgtagcat catcatcagg aactaattgt acaaatacat ttacgccttc tgaatctaac
    25681 tctttaatca tctttatatc atcttttgat acatgcactg ctttagatat attttcacga
    25741 ttatcatctg attttgttcc accaaggttt atagatttaa tagcttttac ctctttagca
    25801 agtctataaa catcttctac agattcacaa agtatcaata agttatattt atcagtaaca
    25861 cctgagttaa gtgcttttat agaatctgct atgcttttca ttactaactt aacattagat
    25921 ggtttagcca ttttaagccc tgccattctt aattcatctt ttaataattc atcacttgct
    25981 atcaatatac agtctgtgct taaaaacttt gtccatgtaa atgctacttg tccatgtata
    26041 agtctgtggt ctactcttac taatttaatc attataattc ctccctaatt tttttgattt
    26101 catccatgaa tgtttttacg tgttctacac taacattccc aaaatctttg taattatctt
    26161 taaagtaact tcctattatt gccatatctc ctaattcaag ctgttttttt gcattttcta
    26221 atgtaactcc agcagctata accaatggaa agtctcctaa atttttacga aacagttcta
    26281 ttttttccat tgatgtttct tgacctgtag cattttctgt aactgctata gcatcacatc
    26341 ttgtcattcc tatttttaaa tcttcttcaa cgtcattttc tgataatact ggctgatatt
    26401 taaaacgtac gcctccaatt aaatacgctg ggcattttga acgttgtaat ttaaaaaatt
    26461 cttctaaagt agcttcgtct ctaggtttaa catgacctac tacagaatca acctgtatat
    26521 aactagcatt atattttgtt gccagttcaa atcccatagt atcaacattt aaacaattta
    26581 ctccatatgg tattgacaaa ttagcttttg atacatattc aagtattctt tctaaatcat
    26641 aataattacc atagtaattt tctaacatta ttccatctac accattttct tcaaagattg
    26701 taatctcttt tttagctctt tcaaagatat cttctggagt atctcctttt aagtgtatca
    26761 ttgctataat tggtttttta cttttaaata ctgataatat tccctttttt ttcataaact
    26821 aaaatacctc ttcttcttct ttaatttgta atgtcacgct ttgtattcct cttctagatt
    26881 cttccactaa ttgttctata tcttcaactg ttaattcatc agaaaacctt gtaagaagtt
    26941 ctaataccat attcaaattc attccagaaa ataacttaac attatgtaga ttagttaatc
    27001 ttaacattgc tgtacataca cttgctccat ataagtcaga taaaattata tattcacatt
    27061 ctatatcttc tgatattgtt ttttctaatt ctaaagcaaa atctgaagca ctttgtccag
    27121 ggtacaaacc atacgaacta acacaatctg ctaaatctcc tactatcatt tttactgcat
    27181 ttaaaagccc ttttgaaaaa tctccatgag atgctaatat tatctttttc atacttcatc
    27241 ctcctactaa atatagatta tatttctgct gatactgcat atttgtactt atcacttctt
    27301 ataaattgct cattgtaata taagacatca ttattttctg tatacaccga ttgtcttact
    27361 ttcaatagtg gcatattact tgcacattta agatagtcag aatattcttt gtttgaatgt
    27421 tttacttcaa atatattttc tactcgttta ggtaatgtgc ttatattatt agatataacc
    27481 tccattaaag atttatcttc taattcttct tttaataaaa acatgtaatc tattctaaaa
    27541 tagtcaactt caaaaataac aggtacttca tctattaatc taagtctttt aatacatatt
    27601 accttctctt ctagttcaat acttaaagct tcagctactt gcttatctgc tttttttatt
    27661 aaaacagaga ttattttggt acttggtttt gcattaatac gatgacaaga attagtaaaa
    27721 ctatttcctg cacatgttgc ttctattctc tctggtattg ttacataagt ccctttacca
    27781 cgttttttac ataatatatc ttcttttaca agttcattaa gtgcatttct cacagaaatt
    27841 cttatatttt atttatgttt atttatattt atttatgttt ttatgagtac atttatttac
    27901 aacctatcta aaacaatttc tttattctat tttatttata tttctttatt tgtattttat
    27961 tttattaaac tgtcatggtg gcaaaaaagg tggcaaaaat aatttgccac ctaaaataat
    28021 ctttatttat tctttctctt ttaaattttt aaaatgttct tcgagttcaa ttaatttatc
    28081 ttcatccact tcatcatctt tactatgtaa cgctgatatt aaacttgaaa ttgaattctt
    28141 atgtatgttc ttcaaaaatt ctttcgtttc aaaccttaga tattttttct cttttactag
    28201 tatattgtaa tgtgtaagtt tgcctatttt ctctgtgctt aaaaattctc ttcgctctaa
    28261 cctttttaaa actgtaaatg tcgttgattc attccattca tatttttctt ccattgcagc
    28321 aactaattcc ctagaagtta ataccttgtt agcttcccaa atatatctca taacttttaa
    28381 ttccgcttct ggtaatttac taattttcat agtctacacc cctttaccga tttatgtata
    28441 tgttaaattc tatatcaata aagttttaca aacaagagaa ttaaggtaaa taattccatc
    28501 caatttttac catatcattc ttttaagttt ttatagtact cttctaatga gtctaacttc
    28561 tctttactta catttttatg cgtagtggta agtgaacgca ttatttttct acttttacta
    28621 gattttagta cctttttctt aaaagcataa tattcttcat tatctattat tattttataa
    28681 taagattgaa attttataat atccctagcc aaataacctt tgtcaattag cctttttagt
    28741 agtattttta ttgtactttt atgccatcca taaatctgtt ttatttcttt tacaatttct
    28801 tttttaggca ttagagtgtc ctttttccat aaatactcca ttactattaa ttctgctctt
    28861 aataattttt ctattaatat ttcttctttc aatttatgtc acttccttaa attcgtctaa
    28921 cttaaattaa taataggagc tttttaagac aaaaacta
//
LOCUS       DAGQWR010000043        28816 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.043, whole genome shotgun sequence.
ACCESSION   DAGQWR010000043 DAGQWR010000000
VERSION     DAGQWR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.043"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            111..665
                     /gene="tcdR"
                     /locus_tag="MIW95_001604"
     CDS             111..665
                     /gene="tcdR"
                     /locus_tag="MIW95_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBY3150934.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            996..8096
                     /gene="tcdB"
                     /locus_tag="MIW95_001605"
                     /gene_synonym="toxB"
     CDS             996..8096
                     /gene="tcdB"
                     /locus_tag="MIW95_001605"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBY3150935.1"
                     /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIVESATNDTLESFREN
                     LNDPRFDYNKFYRKRMEIIYDKQKNFINYYKTQREENPDLIIDDIVKIYLSNEYSKDI
                     DELNSYIEESLNKVTENSGNDVRNFEEFKGGESFKLYEQELVERWNLAAASDILRISA
                     LKEVGGVYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMVKLEAIMKYKEYIPGYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNAFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKKNYFEGSLGEDDNLDFSQNTVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGLDVDSLSTEIETAIDLA
                     KEDISPKSIEINLLGCNMFSYSVNVEETYPGKLLLRVKDKVSELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKIIVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLAECEINVISNIDTQVVEGRIEEAKSLTSDSINYIK
                     NEFKLIESISDALYDLKQQNELEESHFISFEDILETDEGFSIRFIDKETGESIFVETE
                     KAIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDATHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     VIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTAATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELILRDKATKVVDYFSHISLAESEGAFTSLDDKIMMP
                     QDDLVISEIDFNNNSITLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPVITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMNINIELNENDTWVIDVDNVVRDVTIESDKIKKGDLIENILSKLSIE
                     DNKIILDNHEINFSGTLNGGNGFVSLTFSILEGINAVIEVDLLSKSYKVLISGELKTL
                     MANSNSVQQKIDYIGLNSELQKNIPYSFMDDKGKENGFINCSTKEGLFVSELSDVVLI
                     SKVYMDNSKPLFGYCSNDLKDVKVITKDDVIILTGYYLKDDIKISLSFTIQDENTIKL
                     NGVYLDENGVAEILKFMNKKGSTNTSDSLMSFLESMNIKSIFINSLQSNTKLILDTNF
                     IISGTTSIGQFEFICDKDNNIQPYFIKFNTLETKYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVIISPNIYTDEINITPIYEANNTYPEVIVLDTNYIS
                     EKINININDLSIRYVWSNDGSDFILMSTDEENKVSQVKIRFTNVFKGNTISDKISFNF
                     SDKQDVSINKVISTFTPSYYVEGLLNYDLGLISLYNEKFYINNFGMMVSGLVYINDSL
                     YYFKPPIKNLITGFTTIGDDKYYFNPDNGGAASVGETIIDGKNYYFSQNGVLQTGVFS
                     TEDGFKYFAPADTLDENLEGEAIDFTGKLTIDENVYYFGDNYRAAIEWQTLDDEVYYF
                     STDTGRAFKGLNQIGDDKFYFNSDGIMQKGFVNINDKTFYFDDSGVMKSGYTEIDGKY
                     FYFAENGEMQIGVFNTADGFKYFAHHDEDLGNEEGEALSYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGISIINDGKYYFNDSGIMQIGFVTINNEVFYFS
                     DSGIVESGMQNIDDNYFYIDENGLVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFDPETKKAYKGINVIDDIKYYFDEN
                     GIMRTGLITFEDNHYYFNEDGIMQYGYLNIEDKTFYFSEDGIMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8218..8718
                     /gene="tcdE"
                     /locus_tag="MIW95_001606"
     CDS             8218..8718
                     /gene="tcdE"
                     /locus_tag="MIW95_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBY3150936.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAVLLDAMTDEM
                     NAKDEK"
     gene            8797..8958
                     /locus_tag="MIW95_001607"
     CDS             8797..8958
                     /locus_tag="MIW95_001607"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150937.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYMDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9047..9229
                     /locus_tag="MIW95_001608"
     CDS             9047..9229
                     /locus_tag="MIW95_001608"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150938.1"
                     /translation="MRDEGQNDRENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9446..17578
                     /gene="tcdA"
                     /locus_tag="MIW95_001609"
                     /gene_synonym="toxA"
     CDS             9446..17578
                     /gene="tcdA"
                     /locus_tag="MIW95_001609"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBY3150939.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     LLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTIPRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKDSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKEYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPYISSHIPSLSV
                     YSAIGIKTENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENNGTKLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNTKIKLDKDTRNFIMPTITTDEIRNKLSYSFDGA
                     GGTYSLLLSSYPISMNINLSKDDLWIFNIDNEVREISIENGTIKKGNLIEDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNGSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLNNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIESNLVTGWQTINGKKYYFDINTGAASTSYKIINGKHFYFNNNGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSGSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTSIASTGYTIINGKYFYFNTDGIMQIGVFKVPNGFEYFAPANTHNNNIEGQAILY
                     QNKFLTLNGKKYYFGSDSKAITGWQTIDGKKYYFNPNNAIAATHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDSKKYYFNLNTAVAVTGWQTIDGEKYYFNLNTA
                     EAATGWQTIDGKRYYFNTNTYIASTGYTIINGKHFYFNTDGIMQIGVFKGPDGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTYIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNDSKAATGWATIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGPNGFEYFAPANTDANNIDGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINSKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17913..18473)
                     /locus_tag="MIW95_001610"
     CDS             complement(17913..18473)
                     /locus_tag="MIW95_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin regulator"
                     /protein_id="HBY3150940.1"
                     /translation="MFFGNISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAE
                     PWFKMKDDEKKAIEAENQRKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYK
                     YKKVKFEGKVIQVIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGI
                     SAGTITYESTMGGKITIPGIAVEKIN"
     gene            19010..19255
                     /locus_tag="MIW95_001611"
     CDS             19010..19255
                     /locus_tag="MIW95_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBY3150941.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(19472..19741)
                     /locus_tag="MIW95_001612"
     CDS             complement(19472..19741)
                     /locus_tag="MIW95_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBY3150942.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIGQCRKWLEETVSN"
     gene            complement(19953..20255)
                     /locus_tag="MIW95_001613"
     CDS             complement(19953..20255)
                     /locus_tag="MIW95_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150943.1"
                     /translation="MKYSKGFKLIYSLVEYSKKFKVIYSVMKYSKGFKLIYSLVEYSK
                     RFKLIYDVMKYSKEFKLIYSLVEYSKRFKVIYSVMKYSKRFKAIYNAVKYIQGDLK"
     gene            complement(20775..21512)
                     /locus_tag="MIW95_001614"
     CDS             complement(20775..21512)
                     /locus_tag="MIW95_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3150944.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
     gene            complement(21524..22285)
                     /locus_tag="MIW95_001615"
     CDS             complement(21524..22285)
                     /locus_tag="MIW95_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3150945.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPILMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            complement(22285..23208)
                     /locus_tag="MIW95_001616"
     CDS             complement(22285..23208)
                     /locus_tag="MIW95_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3150946.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQRLLDLPKKNVNEALKIVRLENQKNKKVNQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKAKNVVSAQKLLNTKGYMSLIQNGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            23370..24077
                     /locus_tag="MIW95_001617"
     CDS             23370..24077
                     /locus_tag="MIW95_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3150947.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            24122..25528
                     /locus_tag="MIW95_001618"
     CDS             24122..25528
                     /locus_tag="MIW95_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3150948.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILKDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTIKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGVKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVAGIINAHRGKLDI
                     MSIDSQGLKTILKLPLIK"
     gene            27117..27506
                     /locus_tag="MIW95_001619"
     CDS             27117..27506
                     /locus_tag="MIW95_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY3150949.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            28251..28721
                     /locus_tag="MIW95_001620"
     CDS             28251..28721
                     /locus_tag="MIW95_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBY3150950.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT        11432 a         3225 c         4421 g         9738 t
ORIGIN      
        1 ataaaattta atttatttgc cgattatata attataatga ctgatttaat tccaatgttg
       61 tcaaaatttt taaataaatc atcattataa atataagaga ggatgatttt atgcaaaagt
      121 ctttttatga attaattgtt ttagcaagaa ataactcagt agatgatttg caagaaattt
      181 tatttatgtt taagccatta gtaaaaaaac ttagtagagt tttacattat gaagagggag
      241 aaacagattt aataatattt tttattgaat taataaaaaa tattaaatta agtagctttt
      301 cagaaaaaag cgatgctatt atagtcaaat atattcataa atcattactg aataagactt
      361 ttgagttgtc tagaagatat tctaaaatga agtttaattt tgtagaattt gatgaaaata
      421 tcttaaatat gaaaaataat tatcaaagta agtctgtttt tgaggaagat atttgttttt
      481 tcgaatatat tttgaaagaa ttatctggta ttcaaagaaa agttattttt tataaatatt
      541 taaaaggata ttctgataga gaaatatcag tgaaattaaa aatatctaga caagctgtta
      601 ataaggctaa aaatagagca tttaaaaaaa taaaaaaaga ctatgaaaat tattttaact
      661 tgtaattaat gaatttaaag aaatatttac aatagaaatc aaattttaga attaacttta
      721 ttgtaaaatc aataacttaa tctaagaata tcttaatttt tatattttat atagaacaaa
      781 gtttacatat ttatttcaga caacgtcttt attcaatcga agagcaaatt aatcaactga
      841 gtgtcttcaa tttaaaatgt taggaagtga atgtatatga aaacctaagt agatattagt
      901 atattttata aatagaaagg aggatatata aaagagtttt agcatttaga tgtaaaaata
      961 ttcaataaaa atattatagt aaaggagaaa attttatgag tttagttaat agaaaacagt
     1021 tagaaaaaat ggcaaatgta agatttcgtg ttcaggaaga tgaatatgta gcaatattgg
     1081 atgctttaga agaatatcat aatatgtcag agaatactgt agtcgaaaaa tatttaaaat
     1141 taaaagatat aaatagttta acagatattt atatagatac atataaaaag tctggtagaa
     1201 ataaagcctt aaaaaaattt aaggaatacc tagttacaga agtattagag ctaaagaata
     1261 ataatttaac tccagttgag aaaaatttac attttgtttg gattggaggt caaataaatg
     1321 acactgctat taattatata aatcaatgga aagatgtaaa tagtgattat aatgttaatg
     1381 ttttttatga tagtaatgca tttttgataa acacattgaa aaaaactata gtagaatcag
     1441 caacaaatga tacacttgaa tcatttagag aaaacttaaa tgaccctaga tttgactata
     1501 ataaatttta cagaaaacgt atggagataa tctatgataa gcagaaaaat ttcataaatt
     1561 actataaaac tcaaagagaa gaaaatcctg accttataat tgatgatatt gtaaagatat
     1621 atctttcaaa cgagtattca aaggatatag atgaacttaa ttcctatatt gaagagtcat
     1681 taaataaagt tacagaaaat agtgggaatg atgttagaaa ctttgaagaa tttaaaggtg
     1741 gagagtcatt caaattatat gaacaagagt tggtagaaag atggaatttg gcagctgctt
     1801 ctgacatatt aagaatatct gccttaaaag aagttggtgg tgtatattta gatgttgata
     1861 tgttaccagg aatacaacca gacctatttg agtctataga gaaacctagt tcagtaacag
     1921 tggatttttg ggaaatggta aaattagaag ctataatgaa atacaaagaa tacataccag
     1981 gatatacttc agagcatttt gatatgttgg atgaagaagt tcaaagtagt tttgaatctg
     2041 ttctagcttc taagtcagat aagtcagaaa tattctcatc acttggcgat atggaggcat
     2101 caccactaga agttaaaatt gcatttaata gtaaaggtat tataaatcaa ggactaattt
     2161 ctgtgaaaga ctcatattgt agcaatttaa tagtaaaaca aatcgagaac agatataaga
     2221 tattgaataa tagtttaaat ccagctatta gcgaggataa cgatttcaat actacaacga
     2281 atgcttttat tgatagtata atggctgaag ctaatgcaga taatggtaga tttatgatgg
     2341 aactaggaaa gtatttaaga gttggtttct tcccagatgt taaaactact attaacttaa
     2401 gtggccctga agcatatgcg gcagcttatc aagatttatt aatgtttaaa gaaggtagta
     2461 tgaatatcca tttgatagaa gctgacttaa gaaactttga aatctctaaa actaatattt
     2521 ctcaatcaac tgaacaagaa atggctagct tatggtcatt tgacgatgca agagctaaag
     2581 ctcaatttga agaatacaaa aaaaattact ttgaaggttc tcttggagaa gatgacaatc
     2641 ttgacttttc tcaaaataca gtagttgaca aggagtatct tttagaaaaa atatcttcat
     2701 tagcaagaag ttcagagaga ggatatatac actatattgt tcagttacaa ggagataaaa
     2761 ttagttatga agcagcatgt aacttatttg caaagactcc ttatgatagt gtactgtttc
     2821 agaaaaatat agaagattca gaaattgcat attattataa tcctggagat ggtgaaatac
     2881 aagaaataga caagtataaa attccaagta taatttctga tagacctaag attaaattaa
     2941 catttattgg tcatggtaaa gatgaattta atactgatat atttgcaggt cttgatgtag
     3001 attcattatc cacagaaata gaaacagcaa tagatttagc taaagaggat atttctccta
     3061 agtcaataga aataaactta ctgggatgta acatgtttag ctattctgta aatgtagagg
     3121 agacttatcc tgggaaatta ttacttagag ttaaagataa agtatcagaa ttaatgccat
     3181 ctataagtca agactctatt atagtaagtg caaatcaata tgaagttaga ataaatagtg
     3241 aaggaagaag agaattattg gatcattctg gtgaatggat aaataaagaa gaaagtatta
     3301 taaaggatat ttcatcaaaa gaatatatat catttaatcc taaagaaaat aaaattatag
     3361 taaaatctaa aaatttacct gaattatcta cattattaca agaaattaga aacaattcta
     3421 attcaagtga tattgaacta gaagaaaaag taatgttagc agaatgtgag ataaatgtta
     3481 tttcaaatat agatacacaa gtggtagaag gaaggattga agaagctaaa agcttaactt
     3541 ctgactctat taattatata aagaatgaat ttaaactaat agaatctatt tctgatgcac
     3601 tatacgattt aaaacaacag aatgaattag aagagtctca ttttatatct tttgaggata
     3661 tattggagac tgatgaaggc tttagtataa gatttattga taaagaaact ggagaatcta
     3721 tatttgtaga aactgaaaag gcaatattct ctgaatatgc taatcatata actgaagaga
     3781 tttctaagat aaaaggtact atatttgata ctgtaaatgg taagttagta aaaaaagtaa
     3841 atttagatgc tacacatgaa gtgaatactt taaatgctgc attttttata caatcattaa
     3901 tagaatataa tagttctaaa gaatctctta gtaatttaag tgtagcaatg aaagttcaag
     3961 tttatgctca attatttagt actggtttaa atactattac agatgcagcc aaagttgttg
     4021 aattagtatc aactgcatta gatgaaacta tagatttact tcctacatta tctgaaggat
     4081 tacctgtaat tgcaactatc atagatggtg taagtttagg tgcagcaatc aaagagctaa
     4141 gtgaaacaag tgacccatta ttaagacaag aaatagaagc taagataggt ataatggcag
     4201 taaatttaac agcagctaca actgcaatca ttacttcatc tttaggaata gctagtggat
     4261 ttagtatact tttagttcct ttagcaggaa tttcagcagg tataccaagt ttagtaaaca
     4321 atgagcttat cctaagagat aaagcaacaa aagttgtaga ttattttagt catatatcat
     4381 tagctgagtc tgaaggagca tttacttcat tagatgataa aataatgatg ccacaagatg
     4441 atttagtaat atctgagata gactttaata acaattcaat aactttaggt aaatgtgaaa
     4501 tctggagaat ggaaggtggc tcaggtcata ctgtaactga tgatatagat cacttctttt
     4561 cagcaccatc aataacatat agagagccac acttatctat atatgacgta ttggaagtac
     4621 aaaaagaaga acttgatttg tcaaaagatt taatggtatt acctaatgct ccaaatagag
     4681 tatttgcttg ggaaacagga tggacaccag gtttaagaag cttagaaaat gatggcacaa
     4741 aactgttaga ccgtataaga gataactatg aaggtgagtt ttattggaga tattttgctt
     4801 ttatagctga tgctttaata acaacattaa aaccaagata tgaagatact aatataagaa
     4861 taaatttaga tagtaatact agaagcttta tagtcccagt gataactaca gaatatataa
     4921 gagagaaatt atcatattct ttttatggtt ctggaggaac ttatgcatta tctctttctc
     4981 aatacaatat gaatataaac atagaattaa atgaaaatga tacttgggtt atagatgtcg
     5041 acaatgtcgt aagagatgtc actatagaat ctgataaaat taaaaaagga gatttaatag
     5101 agaatatttt atctaaatta agtattgaag acaataaaat tattttagat aatcatgaaa
     5161 ttaatttctc tggaacatta aatggaggta atggatttgt atctttaaca ttctcaatct
     5221 tagaaggaat aaatgcagtt atagaagttg atttattatc taaatcatat aaagttctta
     5281 tttctggtga actaaaaaca ttgatggcaa attcaaattc tgttcaacag aaaatagatt
     5341 atataggatt gaacagcgaa ttacaaaaaa atatacctta tagttttatg gatgataaag
     5401 gaaaagaaaa tggatttatt aattgttcta caaaagaagg tttatttgta tctgaattat
     5461 ctgatgtagt tcttataagt aaagtttata tggacaatag taaacctcta tttggatatt
     5521 gtagtaatga tttgaaagat gttaaagtca taactaaaga tgacgttatt atattaacag
     5581 gatattattt aaaagatgat ataaaaatct ctctttcttt tactatacaa gatgaaaata
     5641 ctataaaatt aaatggagta tatttagatg aaaatggagt agctgaaata ttgaaattta
     5701 tgaataaaaa aggtagtaca aatacttcag attctttaat gagcttttta gaaagtatga
     5761 atataaaaag tattttcata aattccttac aatctaatac taagcttata ttagatacta
     5821 attttataat aagtggtact acttctattg gtcaatttga gtttatttgt gataaagata
     5881 ataatataca accatatttc attaagttta atacactaga aactaaatat actctatatg
     5941 taggtaatag acaaaatatg atagtagaac caaattatga tttagatgat tctggagata
     6001 tatcttcaac tgtcattaat ttttctcaga aataccttta tggaatagac agttgtgtta
     6061 ataaagttat aatttcgcca aatatatata cagatgaaat aaacataaca cctatatatg
     6121 aagcaaataa tacttatcca gaagtgattg tattagatac aaattatata agtgaaaaaa
     6181 tcaatattaa tatcaatgat ttatctatac gatatgtatg gagtaatgat ggaagtgatt
     6241 ttattcttat gtcaactgat gaagagaaca aggtatcaca agttaaaata agatttacta
     6301 atgtttttaa aggtaatact atatcagata agatatcttt taattttagt gataagcaag
     6361 atgtatctat aaataaagtt atttcaacat ttacaccttc atattatgtg gaaggattac
     6421 ttaattatga tttaggtctg atttctttat acaatgagaa attttatatt aataactttg
     6481 gaatgatggt gtctggatta gtatatatta atgattcatt atattatttc aagccaccaa
     6541 taaagaactt gataactgga tttacaacta taggtgatga taaatactac tttaatccag
     6601 ataatggtgg agctgcttca gtcggagaaa caataattga tggcaaaaac tactacttca
     6661 gccaaaatgg agtgttacaa acaggtgtat ttagtacaga agatggattt aaatattttg
     6721 ctccagcaga tacacttgat gaaaatctag agggggaagc aattgatttt actggcaaac
     6781 taactattga tgaaaatgtt tattattttg gagataatta tagagcagct atagaatggc
     6841 aaacattaga tgatgaagtg tactatttta gtacagatac aggtagagct tttaaagggc
     6901 taaatcaaat aggtgatgat aaattctatt tcaactctga tggtattatg caaaaaggat
     6961 ttgttaatat aaatgataag acattctatt ttgatgattc tggtgtgatg aagtcaggat
     7021 atactgaaat agatggaaaa tatttttact ttgctgagaa tggagaaatg caaataggag
     7081 tatttaatac agcagatgga tttaaatatt ttgctcatca tgatgaagat ttaggaaatg
     7141 aagaaggtga agcactttca tattctggta tacttaattt taacaataag atttattatt
     7201 ttgatgattc atttacagca gtagttggat ggaaggattt agaagatggt tcaaaatatt
     7261 actttgatga agatacagca gaagcatata taggtatctc aataattaat gatggtaaat
     7321 attattttaa tgattctgga atcatgcaaa ttggatttgt cacaataaat aatgaagtat
     7381 tttatttctc tgattctgga atagtagaat ctggaatgca aaatatagat gataattatt
     7441 tctatataga cgaaaatggt ctagttcaaa ttggtgtatt tgacacttca gatggatata
     7501 aatactttgc accagctaat actgtaaatg ataatatcta tggacaagca gttgaatata
     7561 gtggtttagt tagagttggt gaagatgtat attattttgg agaaacatat acaattgaga
     7621 ctggttggat atatgatatg gaaaatgaaa gtgataaata ttatttcgat ccagaaacta
     7681 aaaaagcata taaaggtatt aatgtaattg atgatataaa atactatttt gatgagaatg
     7741 gaataatgag aacaggtctt ataacatttg aagataatca ttactatttt aatgaagatg
     7801 gtattatgca atatggttat ctaaatatag aagataagac gttctacttt agtgaagatg
     7861 gtattatgca gattggagta tttaatacac cagatggatt taaatatttt gcacatcaaa
     7921 atactttaga tgagaatttt gagggagaat caataaacta tactggttgg ttagatttag
     7981 atgaaaagag atattatttt acagatgaat atattgcagc aactggttca gttattattg
     8041 atggtgagga gtattatttt gatcctgata cagctcaatt agtgattagt gaatagataa
     8101 aaatacgtta aatatatcct cttatactta aatatataaa aataaacaaa atgatacact
     8161 acataaagtg ttctatctaa tatgaagatt taccaataaa aaggtgaact atgatgaatg
     8221 cacagtagtt caccttttta tatttctaat ggtaacaaaa tattttttta tataaaccta
     8281 ggaggcgtta tgaatatgac aatatctttt ttatcagagc atatatttat aaagttagta
     8341 attttaacta tatcatttga tacattatta ggatgtttaa gtgcaataaa aagtcgtaaa
     8401 tttaattcta gttttggaat agatggagga atcagaaaag tagcaatgat agcatgtata
     8461 ttttttttat cagtagttga cattcttaca aagtttaact ttttatttat gttaccacaa
     8521 gattgtatca attttttaag actaaaacat cttggaatat ctgaattttt ctctatttta
     8581 tttattttat atgaaagtgt aagcatatta aaaaatatgt gcttatgtgg attaccagta
     8641 cctaagagat taaaggaaaa aatagcagtt ttactagatg caatgacaga tgaaatgaat
     8701 gctaaggatg aaaagtaagt aatggtagat ataataaaaa tattaacaaa taaaaagtgt
     8761 tatccaaata agaatagctg aaagttatca taattcatga aactgataat gaaaacgagg
     8821 gagcagatgc caagagacac acaagtatta aatacatata atttcgaagc aagtgttcat
     8881 tactatatgg atgacaaggt agtatatcaa acattggttc acaaagatgg tgcatggtca
     8941 gttggtaaaa tctattaagc tacattagtt acagatatca caaactataa tagttaaaca
     9001 tagaaatatg tgtaaattgt gatggaaatt attcaaaaac acaaaaatac gtgatgaagg
     9061 acaaaatgat agagaaaata agtatcaaac cttaataaat gatttaattg atagtttaaa
     9121 agttatagga aaaatatata aagaaataaa aacattaaaa aaatataaga tatgtttaca
     9181 aattactatc agacaatctc cttatctaat agaagagtca attaactaat tgagtatctt
     9241 taaattgaaa tgttaggaag tgatttaaat atgaaaactt aaattataaa aaatcaatat
     9301 taatttatct ttaaaaaata gaaaggagtg tataagattt attttcaaag tttaaaaaca
     9361 agaaaatcaa tttaaatttc agaaggaata aatgtggtta tagaagtgga tttattatca
     9421 aaaataatag tactaggagg tttttatgtc tttaatatct aaagaagagt taataaaact
     9481 cgcatatagc attagaccaa gagaaaatga gtataaaact atactaacta atttagacga
     9541 atataataag ttaactacaa acaataatga aaataaatat ttacaattaa aaaaactaaa
     9601 tgaatcaatt gatgttttta tgaataaata taaaaattca agcagaaata gagcactctc
     9661 taatctaaaa aaagatatat taaaagaagt aattcttatt aaaaattcca atacaagtcc
     9721 tgtagaaaaa aatttacatt ttgtatggat aggtggagaa gtcagtgata ttgctcttga
     9781 atacataaaa caatgggctg atattaatgc agaatataat attaaactgt ggtatgatag
     9841 tgaagcattc ttagtcaata cactaaaaaa ggctatagtt gaatcttcta ccactgaagc
     9901 attacagcta ctagaggaag agattcaaaa tcctcaattt gataatatga aattttacaa
     9961 aaaaaggatg gaatttatat atgatagaca aaaaaggttt ataaattatt ataaatctca
    10021 aatcaataaa cctacagtac ctacaataga tgatattata aagtctcatc tagtatctga
    10081 atataataga gatgaaactt tattagaatc atatagaaca aattctttga gaaaaataaa
    10141 tagtaatcat gggatagata tcagggctaa tagtttgttt acagaacaag agttattaaa
    10201 tatttatagt caggagttgt taaatcgtgg gaatttagct gcagcatctg acatagtaag
    10261 attattagcc ctaaaaaatt ttggcggagt atatttagat gttgatatgc ttccaggtat
    10321 tcactctgat ttatttaaaa caatacctag acctagctct attggactag accgttggga
    10381 aatgataaaa ttagaggcta ttatgaagta taaaaaatat ataaataatt atacatcaga
    10441 aaactttgat aaacttgatc aacaattaaa agataatttt aaactcatta tagaaagtaa
    10501 aagtgaaaaa tctgagatat tttctaaatt agaaaattta aatgtatctg atcttgaaat
    10561 taaaatagct ttcgctttag gcagtgttat aaatcaagcc ttgatatcaa aacaaggttc
    10621 atatcttact aacctagtaa tagaacaagt aaaaaataga tatcaatttt taaaccaaca
    10681 ccttaaccca gccatagagt ctgacaataa cttcacagat actactaaga tttttcatga
    10741 ttcactattt aattcagcta ccgcagaaaa ctctatgttt ttaacaaaaa tagcaccata
    10801 cttacaagta ggttttatgc cagaagctcg ctccacaata agtttaagtg gtccaggagc
    10861 ttatgcatca gcttactatg atttcataaa tttacaagaa aatactatag aaaaaacttt
    10921 aaaagcatca gatttaatag aatttaaatt cccagaaaat aatctatctc aattgacaga
    10981 acaagaaata aatagtctat ggagctttga tcaagcaagt gcaaaatatc aatttgagaa
    11041 atatgtaaga gattatactg gtggatctct ttctgaagac aatggggtag actttaataa
    11101 aaatactgcc ctcgacaaaa actatttatt aaataataaa attccatcaa acaatgtaga
    11161 agaagctgga agtaaaaatt atgttcatta tatcatacag ttacaaggag atgatataag
    11221 ttatgaagca acatgcaatt tattttctaa aaatcctaaa aatagtatta ttatacaacg
    11281 aaatatgaat gaaagtgcaa aaagttactt tttaagtgat gatggagaat ctattttaga
    11341 attaaataaa tataggatac ctgaaagatt aaaaaataag gaaaaagtaa aagtaacctt
    11401 tattggacat ggtaaagatg aattcaacac aagcgaattt gctagattaa gtgtagattc
    11461 actttccaat gagataagtt catttttaga taccataaaa ttagatatat cacctaaaaa
    11521 tgtagaagta aacttgcttg gatgtaatat gtttagttat gattttaatg ttgaagaaac
    11581 ttatcctggt aagttactat taagtattat ggacaaaatt acttccactt tacctgatgt
    11641 aaataaagat tctattacta taggagcaaa tcaatatgaa gtaagaatta atagtgaggg
    11701 aagaaaagaa cttctagctc actcaggtaa atggataaat aaagaggaag ctattatgag
    11761 cgatttatct agtaaagaat acattttttt tgattccata gataataagc taaaagcaaa
    11821 gtccaagaat attccaggtt tagcgtcaat atcagaagat ataaaaacat tattacttga
    11881 tgcaagtgtt agtcctgata caaaatttat tttaaataat cttaagctta atattgaatc
    11941 ttctattggt gattacattt attatgaaaa attagaacct gttaaaaata taatccacaa
    12001 ttctatagat gatttaatag atgagttcaa tctacttgaa aatgtatctg atgaattata
    12061 tgaattaaaa aaattaaata atctagatga gaagtattta atatcttttg aagatatctc
    12121 aaaaaataat tcaacttatt ctgtaagatt tattaacaaa agtaatggtg aatcagttta
    12181 tgtagagaca gaaaaagaaa ttttttcaaa atatagcgaa catattacaa aagaaataag
    12241 tactataaag aatagtataa ttacagatgt taatggtaat ttattggata atatacagtt
    12301 agatcatact tctcaagtta atacattaaa cgcagcattc tttattcaat cattaataga
    12361 ttatagtagc aataaagatg tactgaatga tttaagtacc tcagttaagg ttcaacttta
    12421 tgctcaacta tttagtacag gtttaaatac tatatatgac tctatccaat tagtaaattt
    12481 aatatcaaat gcagtaaatg atactataaa tgtactacct acaataacag aggggatacc
    12541 tattgtatct actatattag acggaataaa cttaggtgca gcaattaagg aattactaga
    12601 cgaacatgac ccattactaa aaaaagaact agaagctaag gtgggtgttt tagcaataaa
    12661 tatgtcatta tctatagctg caacggtagc ttcaattgtt ggaataggtg ctgaagttac
    12721 tattttctta ttacctatag ctggtatatc tgcgggaata ccttcattag ttaataatga
    12781 attaatattg catgataagg caacttcagt ggtaaactat tttaatcatt tgtctgaatc
    12841 taaagaatat ggccctctta aaacagaaga tgataaaatt ttagttccta ttgatgattt
    12901 agtaatatca gaaatagatt ttaataataa ttcgataaaa ctaggaacat gtaatatatt
    12961 agcaatggag gggggatcag gacacacagt gactggtaat atagatcact ttttctcatc
    13021 tccatatata agctctcata ttccttcatt atcagtttat tctgcaatag gtataaaaac
    13081 agaaaatcta gatttttcaa aaaaaataat gatgttacca aatgctcctt caagagtgtt
    13141 ttggtgggaa actggagcag ttccaggttt aagatcattg gaaaataatg ggactaaatt
    13201 gcttgattca ataagagatt tatacccagg caaattttac tggagattct atgccttttt
    13261 cgattatgca ataactacat taaaaccagt gtatgaagac actaatacta aaattaaact
    13321 agataaagat actagaaact ttataatgcc aactataact actgacgaaa ttagaaacaa
    13381 attatcttat tcatttgatg gagcaggagg aacttactct ttattattat cttcatatcc
    13441 aatatcaatg aatataaatt tatctaaaga tgatttatgg atatttaata ttgataatga
    13501 agtaagagaa atatctatag aaaatggtac tattaaaaaa ggaaatttaa tagaagatgt
    13561 tttaagtaaa attgatataa ataaaaataa acttattata ggcaatcaaa caatagattt
    13621 ttcaggtgat atagataaca aagatagata tatattcttg acttgtgagt tagatgataa
    13681 aattagttta ataatagaaa taaatcttgt tgcaaaatct tatagtttgt tattgtctgg
    13741 ggataaaaat tatttgatat ccaatttatc taatactatt gagaaaatca atactttagg
    13801 cctagatagt aaaaatatag cttacaatta cactgatgaa tctaataata aatattttgg
    13861 agctatatct aaaacaagtc aaaaaagcat aatacattat aaaaaagaca gtaaaaatat
    13921 attagaattt tataatggca gtacattaga atttaacagt aaagacttta ttgctgaaga
    13981 tataaatgta tttatgaaag atgatattaa tactataaca ggaaaatact atgttgataa
    14041 taatactgat aaaagtatag atttctctat ttctttagtt agtaaaaatc aagtaaaagt
    14101 aaatggatta tatttaaatg aatccgtata ctcatcttac cttgattttg tgaaaaattc
    14161 agatggacac cataatactt ctaattttat gaatttattt ttgaacaata taagtttctg
    14221 gaaattgttt gggtttgaaa atataaattt tgtaatcgat aaatacttta cccttgttgg
    14281 taaaactaat cttggatatg tagaatttat ttgtgacaat aataaaaata tagatatata
    14341 ttttggtgaa tggaaaacat cgtcatctaa aagcactata tttagcggaa atggtagaaa
    14401 tgttgtagta gagcctatat ataatcctga tacgggtgaa gatatatcta cttcactaga
    14461 tttttcctat gaacctctct atggaataga tagatatatc aataaagtat tgatagcacc
    14521 tgatttatat acaagtttaa taaatattaa taccaattat tattcaaatg agtactaccc
    14581 tgagattata gttcttaacc caaatacatt ccacaaaaaa gtaaatataa atttagatag
    14641 ttcttctttt gagtataaat ggtctacaga aggaagtgac tttattttag ttagatactt
    14701 agaagaaagt aataaaaaaa tattacaaaa aataagaatc aaaggtatct tatctaatac
    14761 tcaatcattt aataaaatga gtatagattt taaagatatt aaaaaactat cattaggata
    14821 tataatgagt aattttaaat catttaattc tgaaaatgaa ttagatagag atcatttagg
    14881 atttaaaata atagataata aaacttatta ctatgatgaa gatagtaaat tagttaaagg
    14941 attaatcaat ataaataatt cattattcta ttttgatcct atagaatcta acttagtaac
    15001 tggatggcaa actatcaatg gtaaaaaata ttattttgat ataaatactg gagcagcttc
    15061 aactagttat aaaattatta atggtaaaca cttttatttt aataataatg gtgtgatgca
    15121 gttaggagta tttaaaggac ctgatggatt tgagtatttt gcacctgcca atactcagaa
    15181 taataacata gaaggtcagg ctatagttta tcaaagtaaa ttcttaactt tgaatggcaa
    15241 aaaatattat tttgataatg actcaaaagc agtcactgga tggaggatta ttaacaatga
    15301 gaaatattac tttaatccta ataatgctat tgctgcagtc ggattgcaag taattgacaa
    15361 taataagtat tatttcaatc ctgacactgc tatcatctca aaaggttggc agactgttaa
    15421 tggtagtaga tactactttg atactgatac cgctattgcc tttaatggtt ataaaactat
    15481 tgatggtaaa cacttttatt ttgatagtga ttgtgtagtg aaaataggtg tgtttagtgg
    15541 ctctaatgga tttgaatatt tcgcacctgc taatacttat aataataaca tagaaggtca
    15601 ggctatagtt tatcaaagta aattcttaac tttgaatggt aaaaaatatt actttgataa
    15661 taactcaaaa gcagttaccg gatggcaaac tattgatagt aaaaaatatt actttaatac
    15721 taacactgct gaagcagcta ctggatggca aactattgat ggtaaaaagt attactttaa
    15781 tactaacact gctgaagcag ctactggatg gcaaactatt gatggtaaaa aatattactt
    15841 taatactaac acttctatag cttcaactgg ttatacaatt attaatggta aatattttta
    15901 ttttaatact gatggtatta tgcagatagg agtgtttaaa gtacctaatg gatttgaata
    15961 ctttgcacct gctaatactc ataataataa catagaaggt caagctatac tttaccaaaa
    16021 taaattctta actttgaatg gtaaaaaata ttactttggt agtgactcaa aagcaattac
    16081 tggatggcaa accattgatg gtaaaaaata ttactttaat cctaataatg ctattgctgc
    16141 gactcatcta tgcactataa ataacgacaa gtattacttt agttatgatg gaattcttca
    16201 aaatggatat attactattg aaagaaataa tttctatttt gatgctaata atgaatctaa
    16261 aatggtaaca ggagtattta aaggacctaa tggatttgag tattttgcac ctgctaatac
    16321 tcataataat aacatagaag gtcaggctat agtttaccag aataaattct taactttgaa
    16381 tggcaaaaaa tattattttg ataatgactc aaaagcagtt actggatggc aaactattga
    16441 tagtaaaaaa tattacttta atcttaacac tgctgttgca gttactggat ggcaaactat
    16501 tgatggtgaa aaatattact ttaatcttaa cactgctgaa gcagctactg gatggcaaac
    16561 tattgatggt aaaagatact actttaatac taacacttat atagcttcaa ctggttatac
    16621 gattattaat ggtaaacatt tttattttaa tactgatggt attatgcaga taggagtgtt
    16681 taaaggacct gatggatttg aatactttgc acctgctaat actcataata ataacataga
    16741 aggtcaagct atactttacc aaaataaatt cttaactttg aatggtaaaa aatattactt
    16801 tggtagtgac tcaaaagcag ttaccggatt gcgaactatt gatggtaaaa aatattactt
    16861 taatactaac actgctgttg cagttactgg atggcaaact attaatggta aaaaatacta
    16921 ctttaatact aacacttata tagcttcaac tggttataca attattagtg gtaaacattt
    16981 ttattttaat actgatggta ttatgcagat aggagtgttt aaaggacctg atggatttga
    17041 atactttgca cctgctaata cggatgctaa caacatagaa ggtcaagcta tacgttatca
    17101 aaatagattc ctatatttac atgacaatat atattacttt ggcaatgatt caaaagcggc
    17161 tactggttgg gcaactattg atggtaatag atattacttc gagcctaata cagctatggg
    17221 tgcgaatggt tataaaacta ttgataataa aaatttttac tttagaaatg gtttacctca
    17281 gataggagtg tttaaaggac ctaatggatt tgaatacttt gcacctgcta atacggatgc
    17341 taacaatata gatggtcaag ctatacgtta tcaaaataga ttcctacatt tacttggaaa
    17401 aatatattac tttggtaata actcaaaagc agttactgga tggcaaacta ttaatagtaa
    17461 agtatattac tttatgcctg atactgctat ggctgcagct ggtggacttt tcgagattga
    17521 tggtgttata tatttctttg gtgttgatgg agtaaaagcc cctgggatat atggctaaaa
    17581 tatatgtttg acaaaaaatt attcctgtga tactcagaaa ttatttttat ataataaata
    17641 ttgagattta attaaaagtc atgtgttatt gtaatacatg acttttagtt aaaatttttc
    17701 tatcatttaa taatctatta ttcttgacta ttttataata aaattcatat atggaaatat
    17761 taatactaaa taattaatag ttgatagaaa atagataata tgctaaaagc aaaaactaat
    17821 ttagagcctt gtaactgttt atttgcaatt ataaaaacat ctttaaacat attgactata
    17881 atataaaata ttaactataa tacaaaacaa tattaattaa ttttctctac agctatccct
    17941 ggtatggtta tttttccacc catagttgat tcataagtta tagttccagc acttatacct
    18001 cttatagtta tgtaatcatc ctctaacact cttgaaggag ttattgattt tttataacta
    18061 catagtacga ccttatcata atttccagac acagctaatc ttatttgcac ctcatcacca
    18121 tcttcaataa cttgaataac cttaccttca aattttacct ttttgtactt ataatcatca
    18181 ggtgttctag ctaattggtc ataagtaata ccagtatcat atcctttctt ctcttcttct
    18241 tcttttttac gttgttcttc agccttttta gcttcttcag ctttacgttg attttcagct
    18301 tcaatagctt tcttttcgtc gtctttcatt ttgaaccatg gttcagcatc agacaatttg
    18361 ctatttaaag tttctatttc tgttttttga gaatctataa ctgatgtttg attagaaatg
    18421 acctcctcat ggtcttcaga acaagctggt gaggatatat tgccaaaaaa cacaccaaaa
    18481 taaatgccag tagagcaata tcctttgtgc tcttaaagaa tttaattatt ttcttagagc
    18541 ttccttttct ttcattacta aattcgttac cctcattttt tttagaaaac ataatacaat
    18601 ctccctttta ttagatatat tttattttaa aatttaaaaa cttaaaataa ctagttgact
    18661 aaaaattata tattcatatt ttcctaaaat atatttcccc taagcataat tttacaaaat
    18721 aaatcaagaa gattcaatat attttgaaaa atgaggtaaa aatgtcagat tataatataa
    18781 ttttcttgaa ggtgaatttt atatattaaa tgaaacataa agtattttat ttgtataaaa
    18841 ctatattatg atgtaattaa atgattcagt actttagagg tggtaaaata tagatagatg
    18901 aaagagcata aataaagtga tataataaaa aattaaatat taactataat aaaaaaatat
    18961 ctggtactaa gtataataca aaaataatat atcagaaaga aggtgaagta tgggagaact
    19021 acaaaaaatt ccaggggtag ggaaagcaac agaaaaatct ttaatcatgt taggatatac
    19081 aactattaaa tcacttaaag atgcaaaccc agcacaaatg tatgaaaagg aatgtttaat
    19141 gcgtggacag cacatagaca gatgtcagtt atatgtatac agatgtgcag tatattttgc
    19201 atcaactgaa aatccagaac cagaaaaatt aaagtggtgg tattggaaag attaaaaaat
    19261 caaaaaagtt agatataaaa aagagttgaa tatccgttta aataataaaa actaatactc
    19321 aactcttttt tagtcgtaaa ttttatgtaa atctatttgt atggctaaat atacccatca
    19381 ataaatgtat aaatatacca atcaataaat gactaaacat actacctaac aaaggattaa
    19441 ataaattact taacaaagca ttctaaaaca attaattaga aactgtttct tcaagccatt
    19501 ttctacattg cccaatattt accatagggt caaagccttg gtcttttccc ttatttaaaa
    19561 gcatatcaca agggaaagta tcacattcac cacaatgatt aagttcctta ctttcacagc
    19621 aagttttaac cttacattct ccaccccaaa atggtttttc catagttggg caaccagtgc
    19681 aatttacttg ttcttttttt gtacaactgt cacaacagac accacatctt gattcaaaca
    19741 tgttcaaaca tcctttccat tatttaaaaa tattataaaa tgattttaga tgactacaaa
    19801 agtgcaaata gttgatttta agtcatctaa atgttaaaca taaaattatt aatactggta
    19861 aatttataaa aactattcca aaaatatttt tgaatatttc aaaacacaat aaattacttc
    19921 aaatcccttt gaatattcca taacactata gattacttta aatccccttg aatatatttc
    19981 acagcattat agattgcttt aaatctcttt gaatatttca taacactata gattacttta
    20041 aatctctttg aatattctac aagactatag attagtttaa attcctttga atatttcata
    20101 acatcataaa ttagtttaaa tctctttgaa tattctacaa gactatagat tagtttaaat
    20161 ccctttgaat atttcataac actatagatt actttaaatt tctttgaata ttctacaaga
    20221 ctatagatta gtttaaatcc ctttgaatat ttcataacac catagattac tttaaatccc
    20281 tttgaatatt ctataacact ataaattact tttaactttt atataaccat aaagttttta
    20341 tctggcactt taaatggagt ttttatatca aatttgcctt ccatcgaata accaaactta
    20401 atttaattac acattccaag agtcaaatat tcattcaata tatttgataa ttctatgtta
    20461 tagccataat taaatataga ttattaaagt taaatattta tttaatgtat tgaatgattt
    20521 tttagctata tttaattaag tttcattata taatataaaa attgtttttg cattaagttt
    20581 taatataaca cataataatt ttaaataata acattattat atattttttt attttaggag
    20641 tatatctatt atatagtgag tagataaaac atataaaatt caaataaaag atatatattt
    20701 ttaaataaaa gggatataaa tttcaaacca aaaagaatat aaatttcaaa ccaaaaaata
    20761 tctagcattt accattaaga aataacatca ttctttctaa aatttctatt ggcaatttca
    20821 gaaaatacta tagtagtaat aaatgcaaca catattgtaa ctatagtgtc attaacattt
    20881 aaagcagatt gctttataac ccccatagca ctacctaaca tagaataagg gaaaaatata
    20941 ccaacagcag gttttgctac tattaatccc ataccaaata gagaaaggca aagactaata
    21001 ccaataggag ttgcaaaact tctaatcttt atagacatgt ataattgtat agaagctatt
    21061 gaaatagaaa taatccaacc tttagcaatc caaccagtca attctttagg tacactacca
    21121 gaaaaaccaa acatatttcc tgcaactata taaaagataa caaagaaaac ttgtgtaata
    21181 gctgtaagta ttgaaactac tgtaagttta gataaaaaga tattttttaa attaactggc
    21241 agtgtcatta cattattcca attatgattc atatgttcaa gcctacaaat atatgcacaa
    21301 aagattgcga ttaagatagg aaagaaaaat tccccataaa aaagactaac ttgagtccat
    21361 aagctgtacc attctttttt gagtatgcct tgatttagat aaaaattacc agaaccaatc
    21421 aacacactta taagtggcaa agctaataat acataccata taaaagaatt ttttaacttt
    21481 agaatttctc ccaaaactga cttccttaac atttaaatcc ctcctatact tcttttttta
    21541 ataaatgatt tcttccaata taataaatta gcattccaat taagaaaact aaaatcgtaa
    21601 tagaaaaatt catatcaatt ctatagacag atgaacctaa aacattatca taagcataac
    21661 ttaaaggtga taactctagg taataagacc atataaacaa tctacgaata ccttgataaa
    21721 acaaacctga tgtcaaacct ataaaactac caatcatgcc aaatgtaatt gcaatcattt
    21781 gatttggaat tacagttgca atccataggt gtagtgcaat gatagcaata cttacaagaa
    21841 ttgttcctaa tgtgtatttt aaaagtaaag atacagaaat tgactcaata acccctctaa
    21901 aatgtccaaa caacacaata gataacactt gcatcaatat aggtatagat accaaaatag
    21961 cagaacataa aaatttggat aaataaatac tatttaaaga tgtcacagaa gattttaaaa
    22021 gcttccatgt atctccttta tgttctatat cgcttatgcg tgatgtaatt attgatatta
    22081 agataggaaa gaatagacca tttaaacttg caaaactcat aagcatatag gaatattcaa
    22141 atccacctaa catatctgga ttacgtgtaa tagccctatt tgaatttacg atacaccaca
    22201 ttatttcaac agctacaaat aaaaaggtca tcagaataat cttctttctt ttaagtttat
    22261 aaaattctag accaaggtat ttcattataa agtcaccgcc ttaccagtaa gttctaggaa
    22321 tatatcttcc aagctttttt tatgttcttc tattctaaat acatctatat ttgaatggac
    22381 aagcatacca tttattttag ccacatctga gtcttttaaa ttttcaatag tcaagtaccc
    22441 attttgtata agtgacatat atcctttagt atttaatagc ttttgtgcag atacaacatt
    22501 cttagctttg atagcaatct gactttgacc tctattatga agttctttta tgctatcttg
    22561 aaaaaccagt tcgccatgat ttattattcc tattgatgtg gcaatttggt caatttcact
    22621 taaaagatgg ctagagatta gaacagtcat gccgtatttt tctggtagag attttattaa
    22681 ttctctaatt tcctcaatcc ctgctgggtc gagtccattt gtaggttcat caagtattaa
    22741 taatttggga aacttcatta ttgccatagc aatgccaaga cgttgcttca tacctaaaga
    22801 atattgatta acttttttat ttttttgatt ttctaacctt acaattttta atgcttcatt
    22861 tacatttttt ttaggtaaat ctaagagtct ttgcattatg gtcatgtttt caagccctgt
    22921 aagatgacca tagtatgatg gagattctat caaagaacct atatctttta aaattgatat
    22981 tctatttttt tcatttaact ctttacctaa aatattaata tttccttgtg tgggtttagc
    23041 aagtccaaga agcattttaa gagtggtaga ttttccagct ccattaggac ccaaaaaacc
    23101 atatacagct ccttgtggaa ctcttaggtc tatatccttt actctaaata ttttattgta
    23161 ttcctttgaa agtctttgtg ttctaattat catttccata tgtttcatcc ccttttttaa
    23221 tctgtgctta ttgattcttt taacttatat cagtataata acttggttac atttcagtta
    23281 gctgaagttt accttacaat aaccttaaaa gtctatttag gcaaagttgt gaaaatgggt
    23341 atatggtata ctaaaggagg gtgatgatta tggagtcaat aaaagataaa aaaatacttg
    23401 tggttgatga ccacaaggaa ttattaaaaa tgatagatga aatcctaaga aaagagggat
    23461 ttagtagagt atttttagca tcaagttatg aagaagctgt tagggtattt agaaatgtaa
    23521 agccagattg tgcaatttta gatgttgtat tgccagatgg agatggtttc tctattatga
    23581 gaaaaattag agagacatct aagataccag ttatttttct ctctgcaaga ggtgaagatg
    23641 aagatagatt gattggtttg ggattagggg ctgacgatta tatagtaaag ccatttttac
    23701 caaaggaact tactttaagg cttgtgggta tattaaatcg tgtatatgtg cctatagaag
    23761 aagaggagtt gccagtattt aaactgggag attctgttgt tgtgaattta aatagtgcat
    23821 gtgttgagaa agatgggcaa gagatttctt taacagctaa agagcattcg ttgcttttaa
    23881 agttgtatga aaataaaggg agaattgtta ctaatgattc actatgtcaa gccatttggg
    23941 gagatgatat gtatggatat gaaaataccc ttatggttca tgttagaagg gttagagaga
    24001 agatagaaaa aactccatct acacctaaac atcttattac agtaagaggt cttggatata
    24061 agttgatgat taattaattt tagatattag aataaaagaa atcaagagga gtgatttaat
    24121 tttggatagt gtaaagtaca tattgaggcg atttataggt tcaagtatct tgatttcatt
    24181 actttttatt gtattaaata tagcaggttt tcttctgttt tcatatatgc tatttgtatc
    24241 taaatactct gataatttag atacaagtga tagcagagaa ataaaatcaa acttgattat
    24301 gattgaagat aatttgattt taaaagatgg aaagtttatt ttagatgatt ctagtaagga
    24361 agtactacat aaaaataaag tttgggcaat gttaattgat aacaatggag atagaatatg
    24421 gggatttgat ttaccaaaag aaattccaag tcattatact ttgacagatg tagcaaagtt
    24481 ttcaaggttc tttttaaagg actatcctgt gtatgtatgg gaacatccaa atggaatatt
    24541 agtaacaggt tatccaaagg acaaatacac taaatttaac cttacttata cgattaagga
    24601 aatagaacaa tttcctatgt taataatagg tatgtttctg gttaatgcaa tagttacttt
    24661 tttaatagct ctattcatag gtttaaaaat ggtgaaatct ataaaaccaa ttattactgg
    24721 cataaagctt atgtcaaaag gagaaccagt acttttacaa gaaaaaggaa tactaagtga
    24781 tatttcaaaa tctattaata ctgtatctaa agagttgcaa atgaaagatg aaaaacttag
    24841 gaagaaagag gaagctaggt ctaactggat tgcaggaata tctcatgata taagaactcc
    24901 actttcaatg ataattggat atgctggaga acttgaagaa agcagtagcc tttcaaatag
    24961 agaacaagag caagtgtcta ttatatgcaa tcaaggggtt aaaatcagag aattagtaaa
    25021 tgacctaaat ttagtatcta aacttgaata taatatgcaa gcattgaatt gtgataagat
    25081 tagagtttct gcgtttttga gagagcttat ttctgagttt ataaataata atttggataa
    25141 tgacttttat gttgaacttg atatactaaa tgaagatatt attatagatg cagataaaaa
    25201 attattaaaa agggctatta gtaatttaat acaaaatagt ataactcata accaacaagg
    25261 atgtaatata agggttacct ctaagtctga tttagatttt tgctatataa ttgttgaaga
    25321 tgatggaaaa ggtatgagtc aagaaagaat taatagttta ttaaagagcc aaaatggact
    25381 aagtgaggca tatgccaaag gtcaaaatca tggtctaggt attttgatag tagctggaat
    25441 tattaatgca catagaggta aattagatat tatgagtatt gatagtcagg gactaaaaac
    25501 aatactaaaa ttacctctaa ttaagtgata ttttataaaa taataacaga taagaatgaa
    25561 gaatgaagaa tgaaaatatt ttgaaaataa ttttattctt tattgacaaa ataactctat
    25621 tagagtagaa taaggataaa ttcttttctt gtcaagtttt attaaagtaa agagtaactt
    25681 gtgctttatg cgcttatggc tgagttactc atggtatcaa aattttcata gaaataatat
    25741 tttggaatta ttgtaaggtt attaaattct ttagataatt ccattttttt tattttttag
    25801 ttaatattta attttattat tgacaaaaaa attctactaa aatagaatta tggtatagtg
    25861 ttttttaatt ttaagaaaaa atataatgac atcctaaaaa agttttaatt atacgttgtt
    25921 ctgtgtagtt ataatagctt ctatgtaact attgtaagta tattttatta taattattta
    25981 tttaaagtac gataaatgtt ttaaattgaa gtattttgca ccattgagtt ctactaaagt
    26041 agagtttttg tgtagtattt ttagaaataa catatatata tatgtattta tgttgaaata
    26101 tatgggaaat ttataatata attaatgaaa aaatttaaat tttgtaatat ataaataaaa
    26161 aaactctaaa atagaaggat ataattatat agaatgtttc aattaaacaa ggcaaaaact
    26221 ataatcttaa aattaaaatt aacattattc ttaagaagta agaaatttaa gaagtaaaaa
    26281 gtttagtgat aagtggtagt aagcaagaaa tttagaaagt aggaagttta gtgataagtg
    26341 gtagtaagcg agaaatttag aaagtgggaa gtttagtaat aatttgtagc aagtaagaaa
    26401 tttaggaagt aggaagttta gtaataattt gtagcaagta agaaatttag ggagtaggaa
    26461 gtttagtagt aataatttgt agcaagtaag aaatttaggg agtaggaagt ttagtaataa
    26521 tttgtagtaa gtaagaaatt tagaaagtag gaaatttagt aataatttat agtaagtaag
    26581 aaatttagaa agtaggaagt ttagtaataa tttatagcaa gtaagaaatt tagaaagtag
    26641 gaagtttagt agtaatttat agtaagtaag aaatttagta agtgagaagt ttagttataa
    26701 tttatagtaa gtaagaaatt tagtaagtga gaagttcagt aataagttgt agtaaatgaa
    26761 aagtttagga agtaggaagt tcagtaataa gttgtagtaa ataaaaaatt taggaagtag
    26821 gatttactga aatacaaata tttaaaatca aagagtttaa aagaaaggag agctactgga
    26881 caagtatcca aaatagatag ctgagtacaa aatatctcat aaaattaagc aatataatac
    26941 aagagaattt tgacattatt tatatatatt taccgtgtac aaatagctaa atttaggatt
    27001 aagattgaaa aatatcttat aagtctgagc ttatataaat ccgtataaga tgtatttaat
    27061 cgaaaaattt tattatggat attgcttgtc tatattctga gtagcaaggt tttatcatga
    27121 ctataaaaaa gttgccacaa tcagaattaa agataatgaa atttatatgg aaatcagatt
    27181 ccaaagtaac atctagagat attgttttag gaatggagca aaaatatcaa tggaaacaaa
    27241 cgacaacttt gactctttta tctagattag tagtaaaaag atttctaaat tctcaaaaga
    27301 tagataaata tacacattat gaagttttaa ttaaagaaaa ggaatacata ggagttgaga
    27361 caagagattt ttttagaaat attcatgatt cttctataaa aagtttattg ttgtcattac
    27421 atgaaaatat taatttgagc aaagatgata tcttacttat tgaagagtgg ataaaaaatc
    27481 taaaagaaga ggagaaagac gtataagcaa acgtatttct tatagcatta atataaagtg
    27541 tatgtaagtg tatttatgta atatattagt ctacttaagt aatatgacaa atgattataa
    27601 gtggattatg atactataat gtgaatctaa ataaattaaa tataaattga tagtggatat
    27661 tctatttaat taaatttaaa aagctacctt tacttataag tataattaag gtagcttttt
    27721 aaatagaggt agtgtgatta aagagaattt ttaaatataa attgtgaagc tttaatatag
    27781 atgacaatat atattttgca aatatgacac agtgttttcg tatttaagat gataaaataa
    27841 ataggaatta atgaagatat ttaaaaaata aaaataaaga attaatagaa gatattaaag
    27901 agatagagat aaaagtgtta atagaagata ttgaaaaagt aaaaataaag gattaataga
    27961 agatactgaa gaggtagaaa caaaagtgtt aatagaagat attgaaaaaa taaaaacaaa
    28021 agattaataa aaggtactga agagatagag ataaaagtgt taatagaaga tattgaaaaa
    28081 taaaaataaa agattgatat aatatactga agaggtagaa acaaaagtgt taatagaaga
    28141 tactaaaaaa gtaaaaataa aggactaata aaaggtactg aagagataga gaaaaaaatg
    28201 ttaatagaag atactgaaaa aatagaaata aaaaattagg aggaaaattt atgaaatatc
    28261 aggtttcatt attagcagtg aaggatattg aagtatcaaa aaaattttac aaagaattat
    28321 ttgagcaaga aattgaattg gacttaggca aaaatgtaac atttaaagga ggatttgcta
    28381 tacaagaaga cttcgcatgg cttacaggga ttaatccaga tacaattata caaaaatcaa
    28441 ataatatgga actttatttt gaggttgatg attttgacga atttattgaa aaactcaatt
    28501 attataaaga tgtagagttt gtccataagc cactaaaaca tgaatggcaa caaagagtag
    28561 tgcgtattta tgacccagat aagcatatta ttgagatagg agagtctatg gaagttatag
    28621 ctagaagata tttatctgag ggatactctg tagaagaaac ttctaaaata attcaacatc
    28681 caattgaatt tgtaaagcag tgtgaaaata gtaaggaatg attgtttaaa ttataattat
    28741 ttagtagaaa ttaattgtag tggaactaat tcaagataat atataggact agtatttgac
    28801 aaatgtcata aattaa
//
LOCUS       DAGQWR010000044        28091 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.044, whole genome shotgun sequence.
ACCESSION   DAGQWR010000044 DAGQWR010000000
VERSION     DAGQWR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.044"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            29..763
                     /locus_tag="MIW95_001621"
     CDS             29..763
                     /locus_tag="MIW95_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY3150951.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(905..1396)
                     /locus_tag="MIW95_001622"
     CDS             complement(905..1396)
                     /locus_tag="MIW95_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBY3150952.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1745..2128
                     /locus_tag="MIW95_001623"
     CDS             1745..2128
                     /locus_tag="MIW95_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150953.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKVVLKSPEYIGSHPKKPGKSVEFIKKIDNYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNNGRVKQYDG"
     gene            2634..3017
                     /locus_tag="MIW95_001624"
     CDS             2634..3017
                     /locus_tag="MIW95_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBY3150954.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            3019..3369
                     /locus_tag="MIW95_001625"
     CDS             3019..3369
                     /locus_tag="MIW95_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150955.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(3463..3921)
                     /locus_tag="MIW95_001626"
     CDS             complement(3463..3921)
                     /locus_tag="MIW95_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150956.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(3966..4754)
                     /locus_tag="MIW95_001627"
     CDS             complement(3966..4754)
                     /locus_tag="MIW95_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY3150957.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(4893..5330)
                     /locus_tag="MIW95_001628"
     CDS             complement(4893..5330)
                     /locus_tag="MIW95_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBY3150958.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            6152..6661
                     /locus_tag="MIW95_001629"
     CDS             6152..6661
                     /locus_tag="MIW95_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3150959.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVIVIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            complement(7572..8372)
                     /locus_tag="MIW95_001630"
     CDS             complement(7572..8372)
                     /locus_tag="MIW95_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3150960.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(8567..10165)
                     /locus_tag="MIW95_001631"
     CDS             complement(8567..10165)
                     /locus_tag="MIW95_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY3150961.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIIW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            10493..10669
                     /locus_tag="MIW95_001632"
     CDS             10493..10669
                     /locus_tag="MIW95_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150962.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     SILEKELNISKANT"
     gene            10843..11973
                     /locus_tag="MIW95_001633"
     CDS             10843..11973
                     /locus_tag="MIW95_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBY3150963.1"
                     /translation="MNNFEIKTRIKFGEGSLKVLQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKSIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILNNIK"
     gene            12214..12564
                     /locus_tag="MIW95_001634"
     CDS             12214..12564
                     /locus_tag="MIW95_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBY3150964.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKRD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            12572..13021
                     /locus_tag="MIW95_001635"
     CDS             12572..13021
                     /locus_tag="MIW95_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBY3150965.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDDIGLEKLFKYLEKSKNIDEV"
     gene            13123..13698
                     /locus_tag="MIW95_001636"
     CDS             13123..13698
                     /locus_tag="MIW95_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBY3150966.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            13691..15100
                     /locus_tag="MIW95_001637"
     CDS             13691..15100
                     /locus_tag="MIW95_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBY3150967.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSNENGTEVLITIPTQINI"
     gene            15284..16717
                     /gene="eutA"
                     /locus_tag="MIW95_001638"
     CDS             15284..16717
                     /gene="eutA"
                     /locus_tag="MIW95_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBY3150968.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQTIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSVDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            16735..18099
                     /locus_tag="MIW95_001639"
     CDS             16735..18099
                     /locus_tag="MIW95_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBY3150969.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFYKDGKLTELAGDAS
                     VLLG"
     gene            18112..18993
                     /gene="eutC"
                     /locus_tag="MIW95_001640"
     CDS             18112..18993
                     /gene="eutC"
                     /locus_tag="MIW95_001640"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBY3150970.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKTTEVVVDNNAFI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSQETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            19013..19666
                     /gene="eutL"
                     /locus_tag="MIW95_001641"
     CDS             19013..19666
                     /gene="eutL"
                     /locus_tag="MIW95_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBY3150971.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEIAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            19677..20378
                     /locus_tag="MIW95_001642"
     CDS             19677..20378
                     /locus_tag="MIW95_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBY3150972.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRIDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            20381..21850
                     /locus_tag="MIW95_001643"
     CDS             20381..21850
                     /locus_tag="MIW95_001643"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBY3150973.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNIPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            21946..22233
                     /gene="eutM"
                     /locus_tag="MIW95_001644"
     CDS             21946..22233
                     /gene="eutM"
                     /locus_tag="MIW95_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBY3150974.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            22360..23121
                     /locus_tag="MIW95_001645"
     CDS             22360..23121
                     /locus_tag="MIW95_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HBY3150975.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLEDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            23134..23763
                     /locus_tag="MIW95_001646"
     CDS             23134..23763
                     /locus_tag="MIW95_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBY3150976.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            23805..24530
                     /locus_tag="MIW95_001647"
     CDS             23805..24530
                     /locus_tag="MIW95_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBY3150977.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEILEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            24543..24815
                     /locus_tag="MIW95_001648"
     CDS             24543..24815
                     /locus_tag="MIW95_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBY3150978.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            24808..25359
                     /locus_tag="MIW95_001649"
     CDS             24808..25359
                     /locus_tag="MIW95_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBY3150979.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYVVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            25371..26462
                     /gene="eutH"
                     /locus_tag="MIW95_001650"
     CDS             25371..26462
                     /gene="eutH"
                     /locus_tag="MIW95_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBY3150980.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NSGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLRKENV"
     gene            26464..26937
                     /locus_tag="MIW95_001651"
     CDS             26464..26937
                     /locus_tag="MIW95_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBY3150981.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(27057..27158)
                     /locus_tag="MIW95_001652"
                     /pseudo
     CDS             complement(27057..27158)
                     /locus_tag="MIW95_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            27453..27974
                     /locus_tag="MIW95_001653"
     CDS             27453..27974
                     /locus_tag="MIW95_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY3150982.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
BASE COUNT        11091 a         3466 c         4596 g         8938 t
ORIGIN      
        1 tgaatatatt taaaatggag gaaactttat gaaacaaaat aaatatgatg atgatagatt
       61 ttttaataaa tacagtaaaa tggaacgttc tacaaatgga ttagcaggtg ctggagaatg
      121 gcatgtttta aaaaaaatgt tacctgattt caaaggaaaa agagttttag atttaggatg
      181 tggatttgga tggcattgtc aatatgcagt tgagaatgga gcaatttctg ctgttggagt
      241 tgatatttca gaaaaaatgt taaaagaagc tagaaacaaa acgcaacttg ataatataaa
      301 ttacatatgt atgcctattg aagatattaa ttttccaaaa gatagttttg atgtagttat
      361 tagttccttg gcatttcatt atattcaatc atttgaagat atatgtaaga atataagtaa
      421 ttgtctttca aataaaggta attttgtatt ttctgttgaa catcctatat ttactgcata
      481 tggaagtcaa gagtggtact gtgataaaga tggcaataat ctacactggc cagtagatag
      541 atattttgaa gaaggagtac gtaagtcaaa ttttctagga gaagaggtta taaaatatca
      601 taaaacatta acaacttatt taaatacgtt aatgaaaaca ggatttgaaa tattagaaat
      661 tattgaacct caaccagaag aaaaactgtt agatacaatt cctgatatga aagatgagct
      721 acgtagacct atgatgttac ttgtatctgc aaaaaaaaga taagtaaata taaataatca
      781 ttattgtaga aaaaaattgt agaaaataat tacactaatg gagatgatta taccagtggt
      841 attaattaca ccagtagtaa ggaggcaaaa gcattcgttg cttttgccat tttttaagtt
      901 gcaattatac ttctacgtag tatatttcgt ttccattttc ttctataagc tttttcatat
      961 cttcaaattt taaaaataca gtagcagtat tatcatttgg atgtatacct attttatcca
     1021 tttttaataa atccttatca aatacaactt ttacaacagc ctctttatca tttaatatac
     1081 caaaaggagt cacagctcct ttagtaagtt tcaagtattt ttgaagtctt tcttctgatg
     1141 caaaactaag aggtttacta tcaatttgtt gacgaatatc ttttaaatct gcttttttgt
     1201 ctttacctaa tacaactaaa aaatgttctt ttcctttagc atcacgaaga aataggtttt
     1261 ttacaacaaa ttccatatgt aatgttttta attcttccat ttcttcaatt gtataaactg
     1321 gtggatgatt tacagcctca taaggtatgc ttaactcatc taatttttta tatacttcct
     1381 gttgttgatt gttcatgatt tcctcccatt agaaatttat tttatatata aataatatta
     1441 tcacagaaaa ctttaaaaga aaacgtttta tataatatag acaatagatt atatttttat
     1501 aaagaagcaa gaatactcaa taaaacttaa agaaattaaa caatttctaa aaataccttg
     1561 aaaatagaac cttatttgcc atttataaat tatataaaaa gggattatat aaagtcatat
     1621 atgaaaaaac tatattatac tatcttttct ttttggaata aactatactt taaatataga
     1681 tatttaattt aaataaattt tacagtatta atagtaaatt atcttagaaa ggaatgtgct
     1741 ttatatgtct acagacaaag ttgttggaat catagatgaa gaaacggcag aacttgcagg
     1801 aattgaatat actggtaaaa tttatgcttc ttctggtgtt ataaaacata taaagaaaaa
     1861 acatagatgt caactgagta aggatatttt taatgatata atagacacta taaaagtggt
     1921 attaaagtca cctgaatata taggaagtca tcctaaaaaa cctggaaaaa gtgtggagtt
     1981 tataaaaaaa attgataatt atattttagt agctacagaa ttagatacaa aaaaaggata
     2041 tttatatgta tcttcaatgt atgctataaa agaagcaaaa ttagaaagta gagtaaacaa
     2101 tggaagagta aaacaatatg atggttaaca taatattatt aattttcccc aaaatattga
     2161 aaaatattat tatataagtt ataatattaa tatacacgta gatatttatt agttagattt
     2221 gaggttggaa aaggcaccca atacgctact atagagtagt taatagagat gcaggatacg
     2281 ccgccctgcc aaatctaatt aaattaaaaa gagaggatta attcctctct ttttgttttt
     2341 ttgagatact aatttaatct atatagaaag attgaaggtc atttatacta aaagttttca
     2401 gttatataca ttaaactaat taaataagat atttataatc tatatatgat acatattata
     2461 tattaataaa agaaaaatat atagaaaatc cttataaaaa tttaaattgt aatgaatagt
     2521 aagtaattaa aaagaatttt tattgatttt taaaaattta gttagaaact atatcattat
     2581 ggtaaagaat gtattataat gtttagaaat attataataa ggagcgatta aacatgaatc
     2641 tatcgaagaa aatagaagat aaaagaaaag ctaaacagag agctgaaaaa gttaagaaag
     2701 ctaaaattgc tacagctggt gtggtattag gagctgttac tggtgcagtg agtggagtac
     2761 ttttggctcc taaatctgga aaagaaacaa gagaggatat aaaagatgct tcacaacaaa
     2821 ttgctgaaaa gataaatatg aaaactgtag atgtaaaagg caaagtatct gagaagctag
     2881 aggataaaaa aggaaacttt atagaatcaa agaaaaaaat aaaaaaatat atagataaca
     2941 aaaagagtgt tgaaaattct agtgaggaag ataaaataat agatatagtt gaaccagtta
     3001 gtgttactga ggaataatat ggacaataca ataacgatta acataagtat aatatatgta
     3061 ttattagcta tacttgcttg tattgcttta gtttatttaa ttattgtact tgttaaacta
     3121 cataagattt taaataatgt tggaactatg ttaaatgaaa atactcaaaa tataaataca
     3181 actttaaata atcttcctaa tgttgtagaa acttttggag aagtaggaga aaatatgaaa
     3241 gatgtaacag aagtagttac tgaagttaca gcagattttc ttgttactaa agacagtgtc
     3301 aaatcaaata tggatgttat agtcgatata ttgactataa ttaaaaatat ttttctagat
     3361 aaaaagtaat aaatttaata taaaataaga tataatttaa acgaccttaa aataacattt
     3421 ataaggtcgt tttttattag cttgatttga cactatgtgt ttttaataat tggtaatatt
     3481 cctctttatc atttgaacat atatatctat gcttgttgtt tcaattacat ccataagaac
     3541 atctttacat tcaagctcaa aatcttccat tccttctaaa tattcatcat cttcatcaaa
     3601 aaagatataa gaattatata gccattcttc aaatttttct tcaagtatat cccaatacga
     3661 actccaaaat aatccacaac ctgatatata agatgctcta ctttgattta tataaacctc
     3721 atttagaagc ttttgtaatc tactgctata aagaaaatct gctcttccac tgcttgatat
     3781 taattcattt aaatcttcta ttgacattaa ttcaacttta ccacttttat cataaggagt
     3841 ataattttca tcaacccatt tatgtggttt gttttctctg catactgttg ctatcaactc
     3901 tacaactttt gttcttatca tttttttatc ctaacttatt tattttttat aaaataatca
     3961 tgattttata atttatctat ttcttgtaat gctaatttaa cagtttcata tctatcagca
     4021 ccatttatta ctacatcacc ttttacaagt ttattagctt ctccacctac aacataaacc
     4081 tttttaccta aaccttgctt ataactagac gcctcaatta ttaaagaatc tttcaattgc
     4141 caatgtaaaa tctgagcagc cactttatca gcatcacctg tataaacgat tacattttca
     4201 tacactttat tttcccctcc cccttgagaa tttgatatag atttgtttag tataccttct
     4261 gctatcaatt tagcaacttt atctttatta ttaacataat aatctgtatc tgctttacta
     4321 tctacaaaac atacttctat taatattgct ggtgcaatag tatgattaag ccaataaaga
     4381 cctctaacat cagactttgc acccctatct ttgaatactg tagctaacct agtattcact
     4441 ctttctgcat aggttttgcc attattagtc ttatatattg tttctgtccc tactggagtt
     4501 gaagttgtag catttgaatt aaaatgaatc tgaacagcca aatcagtttc ctgtttattt
     4561 gctatagcac attggtctgc taaatgatta cttgattcat caactcttcc tgtataaaca
     4621 gtagctccac ctattttaag ccatttaaca attaagtcat tcagtattct tgtttctttg
     4681 ctttcgttta tatacccaac agctcctgta ccttttccaa ttaaagtgtg ccctggtatt
     4741 attactactt tcaaactaac atctccttta caattaaatt taacttctat ttaatatatg
     4801 cataacctag aattttgttt ctttttatat cttatacttt tattataacc cattttgtaa
     4861 aaatatagaa tattaagtaa ataatgttaa aattataatt ttgtatataa gacattactt
     4921 gattgcaata attttatagc atattcatct attctatatc cagttctata atatatatgt
     4981 ttaattccag cttgaattat agccttagta caatttaagc aaggaaagtg agttacataa
     5041 atagagcaat ctttaactga aataccttct tttgcacaat aataaagggc attttgttca
     5101 gcgtgaatgg ttctaataca atgtccatct ctcatttcat gaccaacttc actacaatgc
     5161 ttatctccag aaatactacc attatatcca gtagacacta ttctgttttc tgaattgaca
     5221 attatagctc ctacataagc tctatcacaa gttcctcttt cagctactgt ttcgcacagt
     5281 ctcataaaat attcttgcca tgagcatcta ttttcaagtt ttttattcaa atcatatacc
     5341 tccaaaattt atatctaata tataagattt ataccttaaa aataatatta aaaacaaatt
     5401 tatacgataa ataaaattta ttaatactgt ttttagtcta aattatttca ttttaaatta
     5461 aagttacaag gaatctaaaa ttatatatat ctttcttcgt aatatgaatg ttacaatata
     5521 aaaaactgtc tcaataaaat agagacagtt ttacagtcaa agtttataat acaagttcat
     5581 atttttcttt aaattctttt acagcttcgg gccatgcctt ttgttctgta ataccttttt
     5641 ctttagctat tttaattgca atctctctta tcattgcagc gccttctaat attcccattt
     5701 ttaatctccc ttcaaatatc tttttgtata gaatataaat agtgctaaga tttattacat
     5761 gctgcatata caatatctat acaacacata acttgtaaat tccttttatt ttcatacgtc
     5821 tttaaccgac atataattat attcaataag ttttaaaatt atgaactcaa ttataattat
     5881 ttttattttt ttagaatcta aacactaaaa attgtaggta aatttgtctt atgcctttat
     5941 aaatatttct acagttttaa ttactggaca tatctatttt tattataaaa agcgcttaaa
     6001 aatcgagaat atccgcgtaa aagctgtata taagcaaaac gattaaagga aaaagaagaa
     6061 atttacttga atgttaaaat gcgttgcttg aagcaacggg cagttttgcc tataatatcg
     6121 gtaacaactg aaagaaaggt ggttagccct aatgttaagc gaaaacatta aaacaatcag
     6181 gaagtcaaaa ggactttcac aacaagaact tgctatcaaa ctgaatgtag tgcgacaaac
     6241 aatatctaaa tgggaacaag gactgtcggt tcccgattct gatatgttga tttccatatc
     6301 agaggtgctt gaaacgcctg taagcacttt acttggagaa actattgttg agtcggaagt
     6361 tgatagctta aaagtgattt ctgaaaaatt agagattata aacttgcaac ttgcacaaag
     6421 aaaaaacatg agaagaaaaa tgtttcattg gttatttatt ttattatgtg cagtcatagt
     6481 aatagttttt gtggtcttag ggggactaaa tagtccttac ttggattgga attataccga
     6541 ccctgaaacc gctgtttttg gagtagcttt tcatgctttt gagtggctat ttgtcagatt
     6601 agtaccgatt gtccttatag gagcaattat tggaatcttt ttgacacgta agaaaatatg
     6661 aagtaactct gctttttata atatagcaag cgttccttaa aagtgtcatt ctacatggca
     6721 atcgaggaca actatgtaag gaagaacctt tttgatttcc aactaagcga agttattgag
     6781 gacgccagta ggcccaaaac cgcattaagt aaagtacagg aagcaagact gctggactta
     6841 tactctcatg cgtcagatgc agaaaatgcg aggtctgtag catgatttac aacgatttgt
     6901 acaacgacta cgagtagaaa tatatggaga taagtattat ctacgaaatc agaaaagaaa
     6961 tgacttgata atttactcat gttataatta ttgatgtgaa attaataaat cgaaattcct
     7021 tttttagatt taacaatact gagtaaggaa aaagtgaatg acttttgcaa ggaggtgtta
     7081 atatcaggca aataaagtct tttgtttttc cagttatatc cttaatattc ttagatcaaa
     7141 ttagcaaagt tcttatagga ttattcttaa tggactttga aattgatata attgggaaat
     7201 ttttaagatt caatcctgtt caaaatacaa aattaagtct ctgctatcta acaaacggag
     7261 cacaaaaatg aaaattaaat attaaatata aagcacagtt aattttgatt aaaaagttga
     7321 ctgtcttttc ttttttataa atttaagcat gggtaataaa gaaatgactg catttaagca
     7381 ttatgtatat aattggaggc aataaaaagc cagcaaagcc cataaaatag agctgtgcta
     7441 gcaacatgat gagttataag aagataatca aaagcttacg ctttatttta atttaaaaaa
     7501 atgattactg taaagaaatc attttttatt ataatcactg atacatcaaa tattctaaat
     7561 aaaattttta tttatctgga attgtatatt ttttctataa tattaactac agcgtacatt
     7621 gcccaagcac aaatagccaa tacaaatact cccatcatta ctaaatctaa tttaaatact
     7681 tgagaaccat atactatcaa gtagcctatt ccatatcttg aaactaaaaa ttctccaact
     7741 attacaccta cccatgccat accaatatta attttagtta aatttattaa atttcctgtg
     7801 tttgcaggta atatgagttt aaataatatt tgagatttag tagctccaaa gctttttaac
     7861 atcttaattt tttcttcatc tatattcata aaataattat aagctgaaag tatagtaaca
     7921 acaacagaaa tcgttacagc tgttacgacg attccttcta ttccagcacc tacccaaact
     7981 ataattatag gagctaaagc agtcttagga agagcattta aaactaccaa aaatgggtct
     8041 aatatttttg ataatttttc tgaccaccaa agagcaattg ctaccaaaat acctaataca
     8101 gtacctataa caagaccaag aacagtttca tatacagata ttccaatatg tttaaataat
     8161 tgaccactaa atgcatattg aataaataaa ttgtaaatat cacttggttt gctaaataga
     8221 aatgtattta taacatttaa gtcagctaaa agttcccaga ttacaataaa accaattaaa
     8281 attaatagct ggtagaataa tacatttctt ttttctcttt ttacaccttt aacatagttt
     8341 atatatcctt cagatctttt attaagcctc atgtgtatct agctccttcc ataatatgtc
     8401 gaaatacttt tgaaaatcag gaactttacg tgttttgaga ggcgtttttg ttccatccat
     8461 gttaagttca atatcgtaaa tattttttat ggtagctggt cttttagata gtattgcaac
     8521 cttatctgat atgcttattg cttctgctat agctaaaaaa gtgcaactaa ttgcttaaag
     8581 aaacagaatt atccacttca gcacccatat aaacaatatt aagatttcct aaactgctat
     8641 cccaaatgat actattaata atagtagata ataacaatct cttatcatct aaactggcat
     8701 tatcaacttc atcattaaat ctcttcaaat tactaacaat aatatcaata ttaatactgt
     8761 cactactaat attgatatta ctagattcca aaatattaat cttatccttt aaaatactaa
     8821 tctcattatt taggctctca atctgggaaa ctatataatc agaagcagta ctaccagtat
     8881 tttttgcaag ctctataact aaatttttaa taagtctttc cttatctaaa atttgatttt
     8941 taagtaaatt agtctcagat ttaaaaatat taaaaacatt atcagtctta atgttattac
     9001 tattgaatgc acttataata gactcttctt tacaattctt aattttatct ataactaaac
     9061 tttcaactaa tggaccatta agatttttac agttgcaact atcagaccca agtgtttttt
     9121 tagtaccaca aatataataa tactttaaat ccttgtcagt ttttttgcct ttataggtaa
     9181 ttctcatatt agaaccacat ttaccacatt ttataagacc agataaaaga gcagtttttc
     9241 cagttgatat ccttggagtt ttatcacgat ttttatctaa ttgattttgt acttcaagcc
     9301 atatttcagg ttctatgact ccatggtgtt ttgcaattgc tgctattgag ttagatgtat
     9361 ttttagcata ggtaaggatt ccacattttc tatctggagt acctacaaca tccataccac
     9421 tatttttaag gtaatctaaa atttcatcat tagcttttac atatgctgga tttctaagta
     9481 ttctagataa agaactaggg tcaaatttac caccacgagg accccttatt ccattattat
     9541 agacatattt atataactta gataaggacc taagttctaa atatttattg tatattagct
     9601 ttacaatctc catttgttca tcatttactt gaagcttata catacttctt tcttttagat
     9661 tttcatctaa gaatgtgatt ttttcactgt tgaatccatg aggtgtattg cctccaagcc
     9721 atctaccact acgggctaat tcatacatgt tgtcttttat acgttctgct attgtttctc
     9781 tttctaattg agcaaataca cttgatataa acatcatagc tcttcccatt ggagttgaag
     9841 tatcaaattg ttctttgata gatataaatg atatgtttag ttcatttaat ttttctatta
     9901 aagtagagaa gtcagataca tttctcgata tcctatctaa tctataacaa actaggtaac
     9961 taaatttctt atctttagca tcctgcatca ttttcttaaa ttgtggtcta tctatattgc
    10021 taccactaaa tccctcatct tcatatatca aaaaatcttt tacaccaata gactttgcat
    10081 aatcaataca taattgtatt tgattttcta tagaatcacc ttttccagta aatcttgatt
    10141 ttcttgaata aattgcagct ttcaaaatta aaacctccta taaattagga tatatatttg
    10201 aaattattta tatacaacct tttaagtaaa cttattagaa taaatttatt aattaggtaa
    10261 atttatataa ttctactttt tataatatat gactatttat tttatttctt tactattcaa
    10321 gttctaaatc aataataaat tttgatattt aattgtatca ggacaaatct ctaatttcat
    10381 atattattaa tataaaatca taggttagtg tgaaaaagtt gaaatttaaa tattttaaaa
    10441 gataattttt tggaactaat aggtataaca aaaatttata gggggattgc atatggaaat
    10501 caatgttata gaaattttcc ctaaagataa agctaaactt aataaaatag aaatggataa
    10561 agctagttgg tttgtaaata taataagtaa aaaatatcct aaagaagctt taaatgaagc
    10621 atttagtatt ttagaaaaag aattaaatat aagtaaagct aatacataag ctttgattat
    10681 tgattttata tactattggt tttatacgct atgacttgac attatattaa tcaaatagta
    10741 tatttttaaa tatagcacaa tgaagtgtta aaagagcaat ggagctccat agataaagtc
    10801 tatggagctt ttttgcgtat ttaacttaag gaggatagag ctttgaataa ctttgagatt
    10861 aaaactagaa taaaatttgg agaaggttca ctaaaagttt tgcaagaatt aaaaaacaaa
    10921 aatgtattaa taataacaga cccttttatg gtgaaatcaa aaacaataga taaaataaca
    10981 tctaatttaa taaactcaac atatcaagtt tttgaagaaa tagtaccaga cccaccaata
    11041 gaagtagtag tagcaggaat agaagcattt aaatctataa atccagacgt aataatagca
    11101 ttaggtggtg gttctgcaat agatgcagca aaatctataa tggacttttc taaaaaaata
    11161 ttaaaaatta acgaagtaga gtttatagca ataccaacaa caagtggaac aggttcagaa
    11221 gtaacatcat ttgctgtaat aacagatagt caaaaaaaat gtaagtatcc attagtgagt
    11281 gatgatttac ttccagatat ggcaatacta gacccagagc ttgtaaaaac tgtaccaaat
    11341 tttataacag cagatacagg gatggatgta ttgacacatg ctatagaagc ttacgtatct
    11401 acaaattcta atgacttttc agatgcacta gctgaaaaat ctataaagtt agtatttaaa
    11461 tacttaataa aagcatataa agatggaaat gacatagaag ctcgagaaaa aatgcacaat
    11521 gcatcttgtt tagcaggtat ggcatttaac caagcatcac ttggcataaa tcattctatt
    11581 gcacatgtat taggtggaaa attccatata ccacatggaa gaacaaattc tatattactt
    11641 cctcatgtaa tagaatataa ttcatcaata gtaggatata ctaacacaac ttactctgat
    11701 acagctaaaa aatatgcaca aatagcagaa ttagtaggac ttgggagtgc taatataaga
    11761 atatctgtta gaaatttagt aaatgaaata aagaaactac aaaaagaaat gaatatgcca
    11821 actaaattga cacaatgcaa attaagttta gaagatataa ctaatgaaga aagagaaata
    11881 gcaaaacttg ctataaaaga tacttgtaca ttaactaatc ctaaggtacc aactgaagat
    11941 gatataataa acatattaaa taacattaaa taactacatt ttattacatg taaatataat
    12001 attatacaaa tagggaaaat gtttttaaaa atataaatta tatattgaca aatataaaac
    12061 ataaaactat aatgaaagta tcacaaaggc gtgatacttt agtagaaagc aatggagctt
    12121 cttaggaata ttaatattat tcttatggag ctccttttat tttaagcaaa tagcttttta
    12181 ataaactata ttcaaagaaa gcagggaata aatttgactg aagaatctaa gcaaagagtt
    12241 attcaagaat atgttcctgg aaaacaggtt acattagcac atatcatagc taaccctaat
    12301 gaagacatat ataagaagtt aggattagta cttgataaaa gagatgcaat aggaatactt
    12361 acaataacac caagtgaagc gtcaataata gcagcagatg ttgctacaaa ggcttcaaat
    12421 gtatctttag gttttataga tagatttagt ggttcagtag taatatcagg tgatgtaagt
    12481 tctgtagaat cagcgttaaa tgatgtttta gaagtcttag gaaatatgtt aaacttctca
    12541 tctacaaaaa tcacaaggac ataataatac aatgaagaat ataatattta tgggaaagac
    12601 aggtagtggt aaaactacct tatgtcaaaa actagaccag ttagaattaa agtataaaaa
    12661 aactcaatca gtggaacttt ataataatgc aatagatact cctggtgagt atatggaaaa
    12721 tagaaaccta tataatgcct taataacaac atcagcagat gctaaaatta tagcaatagt
    12781 gtatgatgcc acacaagaag aaaattatat agcaccaggt tttgccagta tgttttgcaa
    12841 agacgtaata ggaattataa ctaaaataaa taaagctact gaaaacgaag tcaatatagc
    12901 ttatgaaaga ctatcactag caggagcaag tcaaatcttt aaaatagaca cagttgatga
    12961 tattggactt gaaaaattat ttaaatatct agaaaaatct aaaaatatag atgaagttta
    13021 aggggaaata tttttttatt caaaaatatt attaaagtat gataaaataa aaaaatgtat
    13081 aaactatttt aagaagattt aatctttaag cgaggtagga acatggggaa aaaaatacta
    13141 ttggttgatg atgaaccact gttaaggatg gatacaaaag atatattaga aagtcatggc
    13201 tatgaaataa taggcgaagc aagtgatgga tttgaagctg ttgaaatgtg taaaaaacac
    13261 aaacctgatt tagttataat ggatatagag atgccaatac ttgatggtat aaaggcaggc
    13321 aaaataatat gtaaagaaaa tctagctggt ggagtacttt tattaacttc atttgaagat
    13381 aaagaatacg tagaaaaggc taaaagtata ggtgcttatg gatatttagt aaaaacagca
    13441 agtgaaaaat cacttatccc aaccatagaa atgtgtttaa ataaggtaaa tgaatttgaa
    13501 aaaatgaaaa aagatttaga aaaagtaaat tctaaattga atgaaagaaa attagtagaa
    13561 agagcaaaag gtatacttat aagagagttt aagatttctg aggatgaagc atacactaga
    13621 ataagaaaat taagtatgga taaaagaact acaatgatgg aaattgccaa aatgattata
    13681 ataggatacg atgactaata cgataaaaaa aatatcaact gcacaaacat acttaaataa
    13741 taacgaaatt gaaaaaatta tagaagtatc aaaatcctta gatttaatgg cttcttttta
    13801 tgaggctgat gtttttatag atgtactaag taaatttgat gatgaagcaa ttgttgtggc
    13861 acatggtaga cctaaagaaa agtcaatgta ctgtgaaaat gttataggta aaaaggcttt
    13921 aattgaaaat gaaccaggag ttataaagtg cttaaaaaca ggagaaagag taagagatat
    13981 taaagcttta acacaggaat ataaactggt caaacaaaat attcagccaa taacactaga
    14041 tgacaaagtt ttagcagtac taatagttga aaaagatata agtagtgaag ttaagaatga
    14101 gtttaatgct agtgaagacg aagaaaataa aaatttacat cagtttataa aacttatcgg
    14161 aaataatgac tttttagttg ataatttaaa ttcagccata ttaatatttg ataaaaatgg
    14221 aaaattaaaa attaaaaatc gtattgcaac tgagatatac aaaaatcttg ggtttggata
    14281 tgatatacaa gatatgcatt acaatgattt agatatagaa accaacacct ttgaaaatat
    14341 attaatggat gaaaattatg atgagacaaa tgaagtttca ataggtaata tgttttttaa
    14401 aataaagacc atatacatca aagatgatga atttaaggtt tgcaaaatag tacaagatat
    14461 aagtgattta aagagaaaag aagaagaatt agttttaaaa acagtggcta taagagaagc
    14521 tcatcataga gtaaaaaata atcttcatac agtaatatct ataataaaaa aacaaagtag
    14581 attatctcaa aatgatgaag taaagcagtg tttagataac ataataaaca gagtatttgc
    14641 aatactttca actcattatt tactatctaa agaagttgat aataatatat ctatacgaga
    14701 aggtatcaat ttattagtgt caaatattca aggtggctat ttatttagta aggatataga
    14761 tatacgtata actggtgaag attttaaaat tagtggagaa aaagcaacag caatactttt
    14821 ggttataaat gaaatactac aaaattgtta tgaccatgca ttttcggata gagaacatgg
    14881 tactatacaa atattaataa ataaagataa cgactataga aatattgcta ttatagatga
    14941 tggaattgga tttgatagta ataatataaa taaaaatagc ttagggacat atataataga
    15001 tagttatata acacagatgt taaaaggaaa tatagagaga aaaagtaatg agaatggtac
    15061 agaagtatta attactatac ctacacaaat taatatttaa ataataactc atctacaaag
    15121 tagtagtttt aattgagcaa agaggcttaa ggttattaat agattaagtt aattgaatgt
    15181 atttataaaa attataagta tatattcaat ttaacaggta tttattataa ctttaagcct
    15241 ctttgcttgt ttaagacata cttaaatttt aggaggtgag aaaatgagag aagagttatt
    15301 aagtgttggt attgacattg gaacaagtac tactcagtta attttttcta aattaatagt
    15361 agaaaatact gaatcaaatt tttcggtacc tagaataagt atagtagaca aagaaattat
    15421 ttataaaagc aaagtttatt tcacccctct tatttcaaat aatgaaataa atggtcaaca
    15481 aataaaagat atagttgaaa atgaatataa aaaagcaaat atatctaaag aggatataca
    15541 tacaggagca gtaataataa ctggtgaaac agctagaaaa gaaaatgcaa atgatgtact
    15601 tcatactttg agtggatttg ctggggactt cgttgttgca acagcaggac ctgatttaga
    15661 aagtataata tcaggaaaag gagctggagc tcatatttat tcaaaagaac attcaactac
    15721 aattgtaaat atagacatag gtgggggaac tagtaacata gcctcattta aaaggggaga
    15781 agtaaccgat actgggtgtt tggatatagg tggaagactt gtgaagatag ataaaataag
    15841 taaaaaaata acttatatat cacctaaaat agataaaatc ataaaagata ataatttaaa
    15901 tcttcaagta ggcacaatgg ctactgtaga aaatacaaag ccaatagtgg atataatggt
    15961 tgacttatta atggaaagca taggattaaa gaaacagaca cctttgtttg attttataat
    16021 aaccaataaa tcaataagta tagatgaaaa tattaaaaat gtttcattct ctggtggagt
    16081 agctgattat atttactatg atggagaaat aaaagattat tttgaatatg gtgacttggg
    16141 aattttactt gggcaaacaa taaaaaattg tgaatacctt aaaaaagtaa atgtagttaa
    16201 atctatggaa acaataagag ccacagtggt tggggcaggt tctcatacta ctgaagtaag
    16261 tggaagtaca ataacttaca ctagcgaaaa gtttccactt aagaacttgc cagttttaaa
    16321 attatcagtt gatgaagaaa caggtacttc aaaacaaatc agtgaagcta taaaaaataa
    16381 acttaattgg tttaaactag aaaatgattt gcagaaagtt gctatagcca tgataggaaa
    16441 gaaaaatcca acctttttag atatacaaga gtatgctaaa gggtttgtag atggaatgca
    16501 acaaatcata gaaaatgatt taccttttat agtaattgtt gaaaatgata tggcaaaagt
    16561 gttaggtcaa gcaatatatg cattattaaa ttatgaaaaa gaaattgttt gtatagacag
    16621 tgtcaaggtt gaaaatggag actatatcga cattggaaaa cctatagttg aaggtaaagt
    16681 tttacctgtg gtaataaaaa cattagtttt taattaatag aaggaggtaa gtaaatgatt
    16741 ttaaatacaa aattatttgg acgtacttat cagtttaaag gcttatatga tgtaatggct
    16801 aaagctaatg aagaaaaatc aggagataaa ttagcaggtc ttgcagcaga atcagctgaa
    16861 gaaagaatag cagcaaaagt tgttttatca cacataaaat tggaggatat aagaaataat
    16921 cctgcagtgg catatgaaga agatgaagta acaagaataa tacaagatgc tgtaaatgaa
    16981 aaagtttatg aagaaataaa aaattggaca gtatctgaac ttagagaatg gctattggat
    17041 tcaaatacta caagtcttga tataagaaga atatcaagag gattaacttc tgaaatgata
    17101 gcagcagttg caaaattaat gtcaaatatg gacttagttt atgcagctaa aaagataaag
    17161 gttactgctc actgtaatac aactatagga gaacaaggaa cattatcagc aagacttcaa
    17221 cctaatcatc caacagacga cccagatgga atacttgcat cacttcttga aggtttaact
    17281 tttggtatag gagatgcagt acttggatta aatccagttg atgactctgt tgagagcgtt
    17341 actaaagtat taaaaagatt tgaagaagta aaacaaaaat atcagatacc aactcaaaca
    17401 tgtgtattgg cacacgttac tacacaaatg gaagcaataa gaaatggtgc tcctgcagat
    17461 ttaatattcc aatctatagc aggttctgaa aaaggaaatg aagcatttgg atttaatggc
    17521 gacactatag aagaagctag acagttagct ttaaaacacg gtacagctac aggacctaac
    17581 gttatgtatt ttgaaactgg acaaggttca gaattatctt cagaagcaca tcatggaata
    17641 gaccaagtta ctatggaagc tagatgctat ggatttgcta aaaagtatga accattctta
    17701 gtaaatacag ttgttgggtt tataggacct gagtacttat atgatgctag agaagttgta
    17761 agagcaggac ttgaagacca ctttatgggt aaactacatg gcttaccaat gggtgttgat
    17821 gtttgttaca ctaaccatat gaaggctgac caaaatgata tggaaaactt agccatacta
    17881 ttaacaacag caggatgtac gtattttatg ggaatacctc atggagatga tgttatgctt
    17941 aactatcaaa caacaggata ccatgaaaca gcatcactta gagaaatgtt tggactaaca
    18001 gcaataaaag aatttgatgc ttggatggaa aagatgggat tctataaaga tggtaagtta
    18061 acagagttgg cgggagacgc atctgtatta ttaggatagg aggtatttaa tatgaatgag
    18121 aaggatttaa aagcattggt agaacaatta gttggacaaa tggttggaga attagatact
    18181 aatgttgtat ctgagactgt taaaaagaca actgaagttg tagtagacaa caatgctttt
    18241 atagatgata taacagaagt tgatataaga aagcaattac tagtaaaaaa tcctaaggat
    18301 gcagaagctt atttagatat gaaagcaaaa acacctgcaa gattaggtat aggaagagct
    18361 ggtactagat ataaaacaga aacagtttta agatttagag cagaccatgc tgctgcacaa
    18421 gatgctgtat tctcttatgt agatgaagaa tttataaaag aaaataatat gtttgcagtg
    18481 gaaactttat gtaaagataa agatgaatac ttaacaagac cagatttggg aagaaaattt
    18541 tctcaagaaa ctataaataa tataaaatct aaatttggta caaatcaaaa ggttttaata
    18601 ttagttggag atgggcttag ctcagctgca atagaggcaa atttaaaaga ctgtgtgcca
    18661 gctataaaac agggtctaaa aatgtatggt atagattcaa gtgaaatttt atttgttaaa
    18721 cattgtagag ttggtgctat ggaccactta ggtgaagaat taggttgtga agttatatgt
    18781 atgttggttg gagaaagacc aggattagtt actgctgaat caatgtcagc atatatagct
    18841 tataagccat atataggaat ggcagaagca aaaagaacag ttatatcaaa tattcacaaa
    18901 ggtggaacaa ctgcagttga agcaggtgct catatagctg aacttataaa aactatgtta
    18961 gataaaaaag cttcaggaat agaccttaaa taaagtcgag ggagtgtaga atatgaaaaa
    19021 tgatgtaatt cgtcctaata tactaggaat taaaataatt tcgaatataa gtccagaaat
    19081 ggcacaaaaa ctagaattaa aatcacatca caaaagttta ggtttaataa ctgcagactg
    19141 tgatgatgtt acgtatacag ctcttgacga agcaactaaa gcttcagagg ttgatgttgt
    19201 atatgcaaaa tcaatgtatg caggtgctgc aaatgcttca acaaaacttg ctggtgaagt
    19261 attaggaata atagcaggcc caagcccagc agaagtaaga agtggtctaa atgcagtagt
    19321 tgattttcta gagtatggag caacttttat aagtgcaaat gatgatgatt caatagcata
    19381 ctatgctcac tgcgtatcaa gaacaggtac atacctttca gagatagcag gaataagaga
    19441 aggtgaagca ttagcttatt tagtagcacc accacttgaa gcaatgtatg cactagatgc
    19501 agcaatgaaa gctgcagatg ttaagatgtg tgaattattt gcacctccaa ctgaaacaaa
    19561 ctttggtgga gcattactta caggttctca atcggcttgt aaagcagcat gtgatgcatt
    19621 tgcagaagca gtaaaatcag ttgcagacaa tccaacagga ttttaaggat agttagatgg
    19681 atgctatagg cttaatagaa gtaataggat atgttgctgc aatagaagct tctgatgctt
    19741 gtttaaaatc tgcaaatgtt aatattgtaa gaattgataa ggttggggca ggtattgtta
    19801 ctttaacaat atgtggagat gtcggtgctg taaaagctgc tttagaagca ggtgaaatag
    19861 cagcttccag agttggtaca ttaagaacat cacatattat accgagaatc cataatgaag
    19921 taacaaatgc tttattcaaa actaaagaac ctaaagtctg tgaagtttca gaagataaag
    19981 aaaaaaactt tgagattaca ttggaaataa ctgataatgt atcgaaaaat ttatcagaaa
    20041 aagaaaatgt aattttagat gtcaatgaaa acagtggaga tactgaaaat ttatatagtg
    20101 atatagataa aacaaacaaa aaaattgaaa cagttgatga tagtttaaca gaagctaatg
    20161 aggaaaaaat agaaaatact aatgataaag aagtattgga acaagttaaa atagaaaatt
    20221 ccgattttga tagagatgac ctatcaaaga acagtgtaaa aaaacttaaa gctatggcta
    20281 agaaattaga ttcttcaata acttataaag atttaaatgt tcttaaaaaa gaagaattaa
    20341 taaacttagt taaaaagctt agcagaaggg atagatagtt atgacacttt tagataaaga
    20401 tttagtatct atacaagaag tcagaactct tgtaagaaga tctaaagtgg ctcaagagaa
    20461 actttctaaa atgagtcaag agcaaataga taaaatagta aaagctgttg tagatgcagc
    20521 atttaataat gctactaaat tagctaaaat ggcaaatgaa gaaactggat ttggaaaatg
    20581 ggaagataaa gtaattaaaa atacttttgc atctatggga gtttatgaaa ctttaaagaa
    20641 cacaaaaact attggaataa ttggtgaaga taaagaagcc aatacgatag atatagctac
    20701 accagttgga gttgtggcag gtcttatacc ttcaacaaac ccaacatcaa cagtaatata
    20761 caaaactata atatctctaa aagcaggaaa tactatagtt ttctctccac atccaaatgc
    20821 aaaaaactgc atacttgaaa cagttaatat aataagtgag gctgctataa aggctggagc
    20881 tccagaggat tcaatattat gtataactaa tccaagtata gttggaacca atgaattgat
    20941 gaaacataaa gatacaaact taatactagc aactggagga gaagcaatgg ttagagctgc
    21001 ttactcttca ggaacaccag ctattggagt tggaccagga aatggaccag catttataga
    21061 taaatcagca aatgttaaat tagcaatcaa aagaataata gattctaaaa cttttgataa
    21121 tggtgttata tgtgcatcag agcaatctat agtaatagaa aaatctatgg aagaaactgt
    21181 tttaaaagag cttaaaaatc aaggtgcata tgttttaaat gaagaggaaa gtgaaagatt
    21241 agcaaaattc atattaagag ctaatggtac tatgaatcca caaatagttg gtaaaagtgt
    21301 tgaggtaata gcaaatttag cagacttaac aaatatacct aaaaatacaa aagttctagt
    21361 tgcaagagaa aataaagttg gtcacaatgt agcctattca agagaaaaac ttacaccaat
    21421 actagcgttt tatgttgaag aaaatgtaga agctattatg aacagatgta gagaaatatt
    21481 gttaaatgaa ggtgcaggtc atacattctc aatgcatgta aatgatgaag aattggtaaa
    21541 aagatttgcc cttaatatgc cagtatcaag aatacttgtg aatactcctt cagcacttgg
    21601 aggaataggt gcatcaacaa accttgtacc agcactaaca cttggttgtg gtgcaatagg
    21661 tggaagttca acttcaaaca atataggacc acttgattta ataaatgtta aaagagtagc
    21721 ttatggaatc aaagaaatag aagaacttgg aagagataaa tctcaagttg aaaaatcaat
    21781 gtttgactca atggataaag aagaattaat aaatcttctt gttcaaaaac tagtcaaaga
    21841 actagcataa ctaaaataaa caatgaaact tatggctatc gctataaatt tataaaaata
    21901 taaactataa aaaactataa aattaaattt ttggaggata agattatggc aagtgcaaac
    21961 gcattaggaa tgatagaaac taaaggatta gtaggagcaa tagaagcagc agatgcaatg
    22021 gttaaagctg caaatgtaca attagtagga aaagagcaag taggtggagg attagttact
    22081 gtaatggtaa gaggagatgt tggagctgtt aaagctgcta cagatgcagg agctgcagct
    22141 gccgagagag ttggagaact aatatcagtt catgttatac caagaccaca ctttgaagta
    22201 gatgctatat taccaaaggt atcagctgag taatataaga ataattttaa aatttaaatt
    22261 ttgattgaaa aagaggctat ctcaaaaatg agtcttacta ataaactcaa tgtattttga
    22321 ggtagtcttt tttatacatc taataggaga tgtgataaaa tgggtgtttt aacagaaaac
    22381 gaagtaaaaa gaaaaattgg aaaaaacaaa aataatataa ctgaatttta tatagaaaaa
    22441 ggtcaaataa taactccttc tgcaaaatct tatttagctg agagaagtat tgagcttaaa
    22501 tatgttgata aactttcaga tgtaaacaat gaacctaaag aagttgtaaa agaagtagtt
    22561 aaagagaaaa aatataaata tactactgta tttggcgcta aattggatga aaaaccagaa
    22621 catatgactc aattaaatgg aaatctttta gtttttaaag accataaaag aataatattg
    22681 agaggaaaaa tagattctct tgaatcagaa atactaaagg tacagatact ttgtcagaaa
    22741 ctagatatgc caaagcttgt taaggaattg caagaagtat tagactttgt aagaaatata
    22801 atgagatgtg aagttttaga tgaaaagcta gaagacttta aacttttagg attaactcct
    22861 caagaactta gagaacaatc tcactatcct aaaaaatact ttggtatagg acatgaattc
    22921 ccaagttatg aaatgggaga agtagtagta gctataaata gtataagaag taatacacga
    22981 gaggttgaac ttgcagccta tgaagcattt aagggtgagt atggaaatgt tcacagagaa
    23041 gatttaataa aggcacttaa tagattatcg tcagtatttt ggataatgat atataaggtt
    23101 agaactaata agtataagta gttggaggac ataatggaga atctattaaa tgaaatagtg
    23161 gaagaagtaa taactagagt taaaaaagaa gcatttatag aagttgaagc atcaggaaga
    23221 cacgttcact taagtagaga agatgtcgat aaattatttg gagaaggata tactcttaca
    23281 aaactaaaag acctatctca accaggtcaa tatgcatgta aggaaagagt tacaataact
    23341 ggtcctaaag gaagtataaa aaatgtagtt gtacttggac catgtagaaa tgaaactcag
    23401 gtagaaatat ctttaacaga tggttcaact cttggactaa aagctcctat aaaacaaagt
    23461 ggagacttag aaggtacttt aagtattaaa atatcaacac aatatggtga agttgaactt
    23521 gataaggggt taatggttgc taaaagacat atacacatga ctcctaaaga tgctgaaaaa
    23581 ttcaatgtat gtgataaaga gatagttgaa gccaaggtga tgggtcaaag acctttaaca
    23641 tttgatgatg tagtaataag agtgagtgac agttttaaaa cttatatgca tatagactat
    23701 gatgaagcca atgcatgtgg ttattcaaaa ggaactgttg ctaaaataat aaagaaagca
    23761 taaatttaac ctactatata aattacctct agggaggatt taatatgaat tatgataacc
    23821 ttgtagattt aataacggaa gaaatctata aaaaaataaa tagtaatgaa ataaaaatat
    23881 ctaataaacc taaagctgtg attgtatttg aacaagataa caataaattt aacttattaa
    23941 aagatgaatt tgaaatacta gaatttgata aaagtataag ggaatgtgaa atagtgatag
    24001 tttcaagact ttgtatgagg gggctatcga atatagcact tggaaattca acaagtgatg
    24061 aagaaagatt tatactaaaa atgattatga aaggcaaaaa agtatatgtc ctagatgaag
    24121 gaattgaata caaaaaatat aaggatactg caccaaaagc attatataag aaatttatgt
    24181 catttgaaga tgaaatatgt aaatttggtg tagaaattat aaaagactta aatgttataa
    24241 ctaaaaataa gttaaatata aagaaagaac tagaaagtac tagaggttct aaaaaagaag
    24301 atacaaaagt tttagataaa gatgttttaa atttaaccag tgtagaaagt gtaaaaatag
    24361 ataatgaatt tagtttggat ttaaggaata aaaaacttat atcggaagct gacttaagaa
    24421 aaccaactat aaatggagtt aaaaacatat tagttaataa aaaaagtata ataacaccat
    24481 tagctgtaga ttttatgaga atacaccatc tcaaactaaa gaaactgtaa ggaggtaatt
    24541 ttatggatat aggaaaagtt gtaggtaatg tttgggctac aagaaaagat gaaaaattat
    24601 gtggccaaaa acttttagtt gtaaaaattt taaaaactaa agacacatac aaggaaggct
    24661 tcatggttgc agccgataat gcaggagcag gaaatggaga cttagttctt atatccaaag
    24721 gttcctcagc tagagagtcg attgaaaaat ctcatgttcc agtagatgca actatagttg
    24781 gagtagtcga ttcgtttgag gtgtttgatg aataaaagta taggagctat agagtttaaa
    24841 agcattgcta aaggtataga agtatccaat gaaatgataa agaaatcttc agttgatgta
    24901 ttgtacttaa aaagtatttg cccaggtaaa tttctaataa tagtaggtgg agaaacttct
    24961 tatataaatg aatgtgtaga ttatggaata aaacttggag aaggatatat tgtagataac
    25021 ttcgtaataa atgcaatttc acaagaaata ttagatggat ttaaaaataa gtatcaaaag
    25081 ctagatagta tagtctctat aggcgttgtt gaaagtagta aagtatgcgc tggaataaaa
    25141 atgcttgata agactttaaa atctggcgac ttagtattag taaaacttca attatctttt
    25201 gctataggtg gcaaattagt atatgtagta gcaggagatt taagtagttt agaatatgca
    25261 ttaaaagaaa gtgaaaatgt agtaagagaa aaagaagtaa tatataagac tgtaatacca
    25321 tcagtagaca gtcaaataat aaaaagtcta ataaaataat ggggggaatt atgagtataa
    25381 acgaaattat aatctatgta atggtagtct tcatggtact tggagctata gataaatgta
    25441 taggtaacaa atttggtctt ggagaacaat ttgaagaagg tataatggca atggggtcac
    25501 ttgcagttgc aatggttggt gttatatgtt tagcaccagt tcttgctgat gtattaagac
    25561 caattgtagt accagtattt aatatacttg gagctgaccc agctatgttt gctggaagtt
    25621 tattagctaa tgatatgggt ggtgcgccac ttgcacttga acttgcgaaa gaccctaatt
    25681 ctgggttatt tggtggatta atagtagggg ctatgatggg acctacaata gtatttataa
    25741 taccagttgc gcttggaata attgaaaaag aagaccaaaa attcttagca acaggaatac
    25801 ttgcaggtat aataacaata cctattggag cattcgttgg tggattagtt gcaggattcg
    25861 aggtaatgat ggttcttaga aacttagtgc caataataat atttgcagta gttatagcac
    25921 ttggattatt aaaattcgaa aatgctatga taaaaggatt cacatacttt ggaaaaggtg
    25981 tagtaatagt tataacttta ggacttgctg ctgccatagt tgaagcttta acaggaatag
    26041 ttgtaatacc tggaatgaaa ccaatcagtg aaggtataga aatagttgga gatatagcaa
    26101 tagttttagc aggagcattc ccattagtgt ttgttataac aaaggtattt aataaaccat
    26161 taatggcttt aggaaaagtt cttggaatga atgatgtatc agcagcaggc cttgttgcaa
    26221 gtttagctaa ctgtatacca atgtttggta tgatgaaaga tatggacaat agaggaaaaa
    26281 taataaatgt agcattctct gtatcagcag cattcgtatt tggagaccac ttaggattta
    26341 cggcaggatt taattctaat atgataacac caatgatagt tgctaaatta gttggtggta
    26401 taacagcagt attgttagct atggttatcg caaataagac actaaggaag gagaatgtat
    26461 aagatggata tatcaaatat agataaaaat ttaattgaaa cattagtaag acaaataata
    26521 gaagaaaaaa taagtggaac taaagacaca gtagattttg ttagaaataa agacattagt
    26581 ggaataactt ctataaagtt accaactgta aaagttagtg aatctgatag attagataca
    26641 gggaatccaa gtgatgtagt ttacacaaaa gatttattta cattagagga aagcccaaga
    26701 ttaggttgtg gtatgatgga aatgaaagaa acaacttttg attggactct aaactatgat
    26761 gaaatagatt atgtaataga tggaacttta gatataataa tagatggaag aaaggtttct
    26821 gcttcttcag gtgaattaat attcatacca aaaggaagta agatacaatt ttctgtacct
    26881 gattatgcta gatttatata tgtaacatat ccggcagatt gggcatctca aaattaataa
    26941 aaatcaagtt aagttaataa taatcaaata aataatttaa aatacaaact cttaaataaa
    27001 cacacatatt taaaaaagct atgaattaaa attcatagct ttttttataa agaatgttat
    27061 attttagacg tattttttct atatttactg ggagacatat tatatttttt attaaataat
    27121 ctaataaaat gctctacatt tggataacca atactatttt actgataatc ttttccttat
    27181 cactaatctt acattttaaa atattatttt tactcgtaat attatcataa gaatttgttt
    27241 cttatttaaa actaattaga attataaagt ttattgccat gttattaata aaataaatcc
    27301 agttaaatcg agtgctagtt aaagtaataa gaaactttta caatggtttc ctttatcgat
    27361 tgaaataaaa gttaatatgg tataatatct aaagagagaa tcttatcaat tagaatatgc
    27421 atagaaacaa gtggcttaaa aggagacgtt atatggaaga gatagtaaag aaggaacaag
    27481 caagtatgaa tgatattttt ttgtatctta aagctaaatc aaactcatta tataaatttg
    27541 taatattata taatgagttt atcaataaac ctaaagatta tggaactggg gaacttataa
    27601 acatggttga agtacatatg ctaacagcga ttgaagaaaa tccaggaata agtccaactc
    27661 agttagctaa aatatggaac agaactaaag gtgcaatatc tcaaactata tctaaattgg
    27721 agaaaaaggg ttatataact aaagaaaaga aagaaggaaa tgcaaaaact atattactat
    27781 atccaaccac atcaggagta gagttaagta aggcacataa actatatgac accatagatg
    27841 ttacaagaac atttgaatac ttaatcaagg agtgtacatt agaggagata gatagttttt
    27901 ataaagtaat tgatttgttt attaatttat tagaagaatc tccgcaggaa aataatgatt
    27961 ctgaaaaagt gtagctaaga ttgaagttga ataaagagaa atagatatta tagaagtagt
    28021 acttaagtac acattatata aaaatataat aggtatagga ttaaaaagtt tagtggttta
    28081 aagtttaacc a
//
LOCUS       DAGQWR010000045        27647 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.045, whole genome shotgun sequence.
ACCESSION   DAGQWR010000045 DAGQWR010000000
VERSION     DAGQWR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.045"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(134..1051)
                     /locus_tag="MIW95_001654"
     CDS             complement(134..1051)
                     /locus_tag="MIW95_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBY3150983.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIETVKEFWE"
     gene            1313..1690
                     /locus_tag="MIW95_001655"
     CDS             1313..1690
                     /locus_tag="MIW95_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY3150984.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            complement(1846..2295)
                     /locus_tag="MIW95_001656"
     CDS             complement(1846..2295)
                     /locus_tag="MIW95_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3150985.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2473..2877)
                     /locus_tag="MIW95_001657"
     CDS             complement(2473..2877)
                     /locus_tag="MIW95_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBY3150986.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            complement(2990..3856)
                     /locus_tag="MIW95_001658"
     CDS             complement(2990..3856)
                     /locus_tag="MIW95_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBY3150987.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRSTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            complement(4426..4770)
                     /locus_tag="MIW95_001659"
     CDS             complement(4426..4770)
                     /locus_tag="MIW95_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBY3150988.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            complement(4959..5576)
                     /locus_tag="MIW95_001660"
     CDS             complement(4959..5576)
                     /locus_tag="MIW95_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3150989.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKNLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            complement(5581..7752)
                     /locus_tag="MIW95_001661"
     CDS             complement(5581..7752)
                     /locus_tag="MIW95_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBY3150990.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNED
                     TINKILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVS
                     REASILRLNGYSKLDIIARVIKNIFRIMILSSVTALLIYIIYILKLNIGVRACLYFAM
                     FSIICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSI
                     NNCILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLV
                     EEKDAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGK
                     EIFNLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKT
                     KKSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIIDIDNIDDSFYLSYISRCV
                     YFNSKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIA
                     ISNLMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRV
                     DLSNNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            complement(8167..8799)
                     /locus_tag="MIW95_001662"
     CDS             complement(8167..8799)
                     /locus_tag="MIW95_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBY3150991.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINLENLHIWLENGVDVCGFGGLLTKGTIEDITENAKKIREIIKNIREI"
     gene            complement(8905..9810)
                     /gene="dapA"
                     /locus_tag="MIW95_001663"
     CDS             complement(8905..9810)
                     /gene="dapA"
                     /locus_tag="MIW95_001663"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBY3150992.1"
                     /translation="MKYKGIICAMITPFDEKQNINSQATCKLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVIDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEVTQNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            complement(9815..10414)
                     /locus_tag="MIW95_001664"
     CDS             complement(9815..10414)
                     /locus_tag="MIW95_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBY3150993.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIVLQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            complement(10472..11743)
                     /locus_tag="MIW95_001665"
     CDS             complement(10472..11743)
                     /locus_tag="MIW95_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBY3150994.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            complement(11770..12057)
                     /locus_tag="MIW95_001666"
     CDS             complement(11770..12057)
                     /locus_tag="MIW95_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3150995.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            complement(12072..14159)
                     /locus_tag="MIW95_001667"
     CDS             complement(12072..14159)
                     /locus_tag="MIW95_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY3150996.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPYNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIYLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            complement(14726..15580)
                     /locus_tag="MIW95_001668"
     CDS             complement(14726..15580)
                     /locus_tag="MIW95_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBY3150997.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            complement(15612..16124)
                     /locus_tag="MIW95_001669"
     CDS             complement(15612..16124)
                     /locus_tag="MIW95_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBY3150998.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDKKKGN"
     gene            complement(16117..16341)
                     /locus_tag="MIW95_001670"
     CDS             complement(16117..16341)
                     /locus_tag="MIW95_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3150999.1"
                     /translation="MNEKPSFKQIEIILAVIFLVLTYYKIGLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            complement(16401..17354)
                     /locus_tag="MIW95_001671"
     CDS             complement(16401..17354)
                     /locus_tag="MIW95_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBY3151000.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLESGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            complement(17382..18770)
                     /locus_tag="MIW95_001672"
     CDS             complement(17382..18770)
                     /locus_tag="MIW95_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151001.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPNNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKAGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            19043..19924
                     /locus_tag="MIW95_001673"
     CDS             19043..19924
                     /locus_tag="MIW95_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY3151002.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            complement(20111..21103)
                     /locus_tag="MIW95_001674"
     CDS             complement(20111..21103)
                     /locus_tag="MIW95_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBY3151003.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKKIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(21108..21848)
                     /locus_tag="MIW95_001675"
     CDS             complement(21108..21848)
                     /locus_tag="MIW95_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBY3151004.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLVGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGDTKVEDIGVLYKIMKELLG"
     gene            complement(21913..23022)
                     /locus_tag="MIW95_001676"
     CDS             complement(21913..23022)
                     /locus_tag="MIW95_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBY3151005.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFL
                     IIEELVNKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEINFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDNIYNRIKNIVES"
     gene            complement(23040..24173)
                     /locus_tag="MIW95_001677"
     CDS             complement(23040..24173)
                     /locus_tag="MIW95_001677"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBY3151006.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNHIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGEFY
                     EDTKKYKTNVYALINIAKQGITSQDELSNMMNIDEYELKNAVRKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEAKERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGNSIEENE"
     gene            complement(24175..25269)
                     /locus_tag="MIW95_001678"
     CDS             complement(24175..25269)
                     /locus_tag="MIW95_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBY3151007.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMVSKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAKEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNIFKRGDVVIGNDDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            complement(25406..26707)
                     /locus_tag="MIW95_001679"
     CDS             complement(25406..26707)
                     /locus_tag="MIW95_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY3151008.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKATLDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            complement(26931..27632)
                     /locus_tag="MIW95_001680"
     CDS             complement(26931..27632)
                     /locus_tag="MIW95_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY3151009.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
BASE COUNT         8968 a         4930 c         3050 g        10699 t
ORIGIN      
        1 cttaaaactt ttaaatattg aaatatttct ttatatatat tattgtatat ttcttataaa
       61 ttattgtcaa tttatttttt cttaaaaata aaataattgc ataatttatt tcaaattatg
      121 caatttataa aatttattcc caaaactctt ttactgtttc gattatctca ctataaggca
      181 ataaagtcgc ttcgattatt gatttatttg tcttttctaa ataatactta atcatataat
      241 atccacaagc atatcctgca caataaggca accctactgg aaaatatcct tgcattttag
      301 ctatatcatc accataaaga taagatgtta tattttgaaa tccagtttct tttaaagcac
      361 tttttattat tggctttata taagtattta atgtttcgat atctgttcta ctgacccaag
      421 gtcctaacat ttcctctcca aacatccatg ttgcaaaatt ttctgcaaga ccttcattta
      481 tcatcatttc ttctaatgtt atattattat tccactctat aaactgaaat ctaatattgt
      541 gattacattc atgtgctatt aatactggta atctattgat agtatattca ttaggaacca
      601 atgatagaaa tatatatcca ggaatcccac catcacccca gtatccatca cttaatattg
      661 tataaggact atttggattc gccaataata ttgaatattt atagtcctta atgtttaaat
      721 catacccttc ttttataaaa gcatttatag agttttctat ggtttctttg caagtgcccc
      781 aaattttatc agcagataac acatttatat tctttttttg cttttcatca atatccttag
      841 gcgataaaac ccctaacatt tcacttgcta taattacatc atatccacct ttctgtctag
      901 caactattgg aacattcata cacttccact taaattcaaa aggcttcatc aattcatatc
      961 tgtatatatc ttcttttttg tttattggtg catccattat cttcttgtat attttatctg
     1021 agcgaatttc tataatattc atatttacca tctcctttaa gacaaagtat atacaattac
     1081 gtagcgtcaa tgtcaatata cattttttat aatgtcacct taatcttttt attatatttt
     1141 atcctgtaaa ccttccgtac atataacatt tttttaaatt aataaatttg tataatataa
     1201 taataaaaaa ttatatactt caaggtttcc ctatgataca atttactaaa tcataattta
     1261 gattatcagg tgcaactata aattattcta tatcaaggag gatatattgg tcatgctaac
     1321 tccatattta atatttaatg gtacttgtga aaaagcattt aatttttatg ctgaggcttt
     1381 cggaggagga aaaactatat ttgcgcgatt agacagcaat ccaaacaatc ctattatgca
     1441 cgcaagtgtt actttcacaa aatacgaagg ttgtataatg ggtgcggata cagacaagcc
     1501 tgttgtaatt tctggcatgg cgatttgtgt tgttctacca tctcgagaag cgatagaaga
     1561 aatatctgta aaacttgccg agggtggtac acttgtacaa gaatttttac cacacccacc
     1621 accacatcaa aatgatggcg ctgctgaagt acttgatagg tatgggtata cttggtattt
     1681 aagtacatag taaaaataaa aaaactacat ctaataatca taatatagaa cagataaatt
     1741 tcaatttaat agtgtagtaa aaaagtcgtg tcctaagact gtcacgactt ttatttgtat
     1801 aaaaatttaa gtaaatattt ttgcgaataa gtgagaagta taactttaaa ttaatttcat
     1861 atgtagtatt ggaaatgggt taccttgtcc atctatttca gacctatcaa acactttaaa
     1921 tcctaagtga atataaaatc ctacagcttt tttattctgc tcatttacat caacatattt
     1981 tacattgtat ttatctatag catattttat taatttttta ccaataccat ttcctctata
     2041 tttatcttct ataaagagca tctcaatttt atctttatct attcctataa agccctttat
     2101 tatatcatta tcttttatac aaactatatt ttctacttct ttaagtccaa tttcaactaa
     2161 tggtatcaaa gattcaatat ctttttctgt taaaaaatca tgcgttgctc ttactgatga
     2221 ttcccagact tgcaacattt cgtcatattc tgttgaattt atttgtttca tttcaagaat
     2281 tattccagat ttcataaaat ttcctcccta tatcactttt aattgtataa cactttttta
     2341 tacaaaactt agaaaggact acgaaggtta agtatcatag tcctaattaa gtttaaatat
     2401 tatctattat atatttttaa catttattat atactataat ctagcaaatt tactacaata
     2461 atccatatca gtttatacta aaggtgcttc tatttgagta gtaaaaagga atttatgaca
     2521 atgaccatca ttaacagtag tttctccaca tattaagtga atgtgtttat cagttccagg
     2581 tattctaatt gctggccctg ttgtaacaca tatcttatgg aaatggtcaa ggaaatctac
     2641 attatcattt attttatgaa catgatttgt acctccattt attggtatag cttcaccagt
     2701 aacccctgct gcacggtggt tatgtctatc ctcacattct tctgctaact taacactttc
     2761 tgaatattca tgaacatgtg tcatttcctc ataatcatta tagcacttgt tgcacttatt
     2821 gtatttataa tcatcataca cataatctct ttcacattta taattttgat aactcatttt
     2881 tatatctctc cctatattat attaagaaat tcttgtaaag aatctctaat gtataatatg
     2941 aattaaagtc aaaaaagttc atattttgta aaattatgta ttaatttaat tatttctccc
     3001 agtatttagg gcgtattagt gaagctggta acttcgtact agaatctcct tttgcagtat
     3061 ttacttgcaa ttgtgttaaa aatatactat tttcaagatt agcacctcta atatcacaat
     3121 ctcttaagtc tgctcctatt aaatttgccc cactcaaatc tgtacttctt aaatttgctg
     3181 ctattagaaa tctacctctt aaatctgctc ctattagatt tatttttcta aggtctgctc
     3241 ctattaaatc tatattttta cttaattttt tcatattttt aagagtagaa gtcttgataa
     3301 atgatttaac ctttcccatc acagactcac tcgcttgtga taataataaa ttaacctttg
     3361 ttctatgtga tatgatatcc aaattaatta tctgttctgg gctcatatta gttatctttt
     3421 cagtttcttc aattatcaaa tcaatttttg atttgattgt atctgatata tttaatacag
     3481 atgcttcttt aagataccat aacatttcat gtaattgtct cataattaaa aacacttcag
     3541 acatttgatt tgttaattct ggtgattgca tccagtctat ccccttatat gttacttgtg
     3601 ctactttttg acctgaacca aaacaatcat atgcagtaca ccctttaaaa cctcgcttca
     3661 taagactatt atgtacagaa catttaaaat ctggttgtaa attaatacag ggttttcctg
     3721 attccttatc gataggaaat ccatctgaag cagaaaaata aagagctaca caacaaagtc
     3781 caaaacactt cttacaatca atctttagct cctcaaataa cttattatca tatatatttt
     3841 tatgtatatc tatcatattt tttctcctct taagctttat ttttaatatt atattatcta
     3901 taactaaatg ataatcaagt cccttattta acagttctta ttcttaatta taatttaagg
     3961 tattatttat tccaatagca tacataagtt tctttatact tgtaacaata cctttatact
     4021 aagacttatt ttaaatgtag cactgtaata ataaattgtt tatatactca taatgtattt
     4081 gataaattgt aaatctttat agaagttgaa atttttaatg attttagaat caagatataa
     4141 taataaatac aatttataaa atcagaaata tataaattaa ctatacaaac ctaaataaca
     4201 tagcatttaa attcacaatt tcagtataaa tagtacaaag tatattaatt taaataagat
     4261 tggcttattt gtagaggaga gaatattttg gtgttcaata aaatttatat ttaataaaac
     4321 cataagaaaa tataaagata aataaacaaa cttttattac ttaatagatt cttatggttt
     4381 tatactgtac tgaaaaacaa taaacactac agcttattta taatattacg cttttttaac
     4441 aacagaagac tttagcttca tagctccaaa accatctatc ttacattcta tatcatgtcc
     4501 atctgatgag tcatggatta aacggatatt tttcactttt gttccaatct ttatagatga
     4561 tgaacttcct tttactttaa ggtctctaat tactgtaaca gaatctccat catttaatac
     4621 atttccattt acatctttga taatatttga atcttcttca gctcccaatc ctaatgccca
     4681 ctcatgagag cattctggac aaactaaaag agttccatct tcataagtat attctgaatt
     4741 acatttcggg caatttggta aattttccat ctttatatgt cctccatttg ttatttttaa
     4801 atttcttatt atataaaatt tcttttgagc ctttatgata tcatattata cttatattta
     4861 caaactacta atttacttaa tgtttaaatt ctattcgtat tattattaaa cttgatttag
     4921 ctgtaatctc tttcttatat ttattatagt tgatattatt acaattttat aactctatct
     4981 gcctgctccg ataactctaa atcatgagtt acaaatacga tagttttacc attcttattt
     5041 agatttttaa gtatatcaaa tactatgtct ctattttttt tatctaaatt acctgtaggt
     5101 tcatctgcaa atattacttc acattctttt aatattactc ttgctaatgc taccctttgt
     5161 tgttctcctc cactcaattc gtatatcttt tttttattgt attttctaag acctacagac
     5221 tcaagtgcgt aatttatttt ttcattatgg ttgatatttt tttgatactt taaagcaatt
     5281 ttcaagttat tttcaacatt ttcattctca attaaagcat aattttgaaa tagatagcca
     5341 taaaaatccc tttgtaaaat tcttatctgt ttactatttg gattttttat attatttatt
     5401 ataatatcac cagaattagg tttttctaat aatcccatta tattaagtaa agttgtctta
     5461 cctgaaccac tatttcctgt tatcgctaca aactcgccct tagaaatttc taggttaaat
     5521 ttgtctatta tagatttatc tttaaatttt ttagatatat ttattaatga aatcatcttt
     5581 ttaatgttct ccttttataa ttttattaaa tgaactattt gagataatct tgtctaatac
     5641 aatagataca attacttcta taactaatat cagaagtcca aataagataa tattattact
     5701 taaatctact cttgttgata taatgcctat tggaataatg tttgttaata ttaatgagac
     5761 tattaacaac atacttctag ctgtagttga ataaccaaat atttttttaa tatataactt
     5821 atatttattt ctttcatagt agctagctac tatattgtag gtaatcatta agtttgatat
     5881 tgctattatt attatagtaa aaatttcact attcaaatat ttttctagtt tatttatttc
     5941 taaaccatac tcattataaa cagcatgtaa actttggata tgcgcttcta caccttgagc
     6001 ttcaataatg ttacttatat ctgcaagggc atctaatttc ttagaattaa aatatacaca
     6061 tctagatata taagaaagat aaaaagaatc atcaatatta tctatgtcaa ttattgctat
     6121 tgggtcatca atgagattcc tattgtcatc cataacaagg gagttataag taaaatagct
     6181 ttgattatta tctacatata ttatattaac atttaaatct gacttctttg ttttatttaa
     6241 ctctctattt aactctttat tgtacatatt ttctacttct attttttcaa aataaaataa
     6301 ttctctaaac tcttttttaa tctcattttc atgtacttgt aactttttag gaactaataa
     6361 atttatagtc ttatcgtcat aatcgattag attaaatatt tctttgccat ttgagtaaat
     6421 agggttaact ttaagatagt ttttatttat ttttatattt ttaccatatt gacttacttc
     6481 tgggttttta ccctttgaat ttatttcata catgtaactt ccatcttcaa gtttttcaaa
     6541 attagttgaa tctattaaga aagcatcttt ttcttctacc aaatttttat agaaaccttt
     6601 aatcttggca tttttaatta cttcttcctt tcctaaagat tgttcgcctg tatctttaag
     6661 agtaagatta taaacatttt ttgctttatc ccactcagat agatttccta actgagtttg
     6721 tagcatatta taatttaaaa tacagttatt tatggataat ataagaaatg atacaaatac
     6781 aattttcaat gtgaaatgca ttatatttac aaaaccataa gatttttttc catttagaga
     6841 tagcacatat ttagaacttc tactattaaa accaactatt aatatagata tcaaaatatt
     6901 tatcaaaata catataatcg aaaacatagc aaagtataag catgctctaa cacctatatt
     6961 taattttaaa atatagatta tatatattaa taatgctgtg acagatgata aaatcataat
     7021 tctaaatata ttttttataa ccctagcaat tatatctagc ttagagtatc catttaatct
     7081 taaaatagaa gcctctctac tcacactaat tgaataatgt attattgtag ccaagaaaca
     7141 aaggataatt agtattaaat ctctaattat tgaaggattt aagtataaat ttgagttaac
     7201 atatctatca tgtaatcttg ctacaaaaat acttctgtct acatttagtt ctcttaatat
     7261 tttatttatt gtatcttcat tttgagttga aatatataag attcctgatt ctccaacttc
     7321 acttatttta ccaaaatccc taattaaaat tgatatgtct ttactcatag ttgagaattt
     7381 acctacttga ttactattat tactattatt agtgtttata ttactaataa attcatttga
     7441 attagtatta ggaaattttc ctgattttaa ctttatattg ttattcaaac ttgtatcagt
     7501 cgtatatata gataattttt cattattatt aaaaacatac tttgatatag ccacattatt
     7561 ttcatctgca atatctttaa tccacttact ataattttta tttaactttt tataatcttt
     7621 aaacattaca gatatacttg ttgtatcgtt atataaaata ttattaattt cattatcttt
     7681 aactatattt agacctaata aagatactat taatagttca attgtaaata atacatatat
     7741 tatctttttc aattatagct ccctcctaat tgtttttatt ctaaaagcaa aatatatttt
     7801 actttagtta aagtataatg ctttgtacct tttctaaaca actactatat tgtcacattc
     7861 taaatggcaa gtgtctattt cgtcacaaag ataaaaataa ccttaaaaca gttaattttt
     7921 tataatcaac tgttttaagg ttatttttta tttttagcaa acaatatttt aatagtggta
     7981 caaaattagt ataattcatt aatatagatt ctagtatttt tagcaatcaa ctaaaataat
     8041 atcttattta aaacaaaatt taatttaagt tcaatataaa ctttacctca actagaaaat
     8101 atttcttcta acttaggaat taatgtgtgg atatctcgtc aatagaaaac taattcatcc
     8161 tccatgttaa atttctctta tatttttaat gatttcacga atctttttgg cattttcagt
     8221 aatatcctca atagttcctt tagttaataa accaccaaat ccacatacat caactccatt
     8281 ttctaaccat atatgtaggt tttcaagatt aattcctcca cttgctaaaa ctggcatatc
     8341 aggtattggt gtcttaaata cctttactag tttaggtcca tagaaatctg atattgggaa
     8401 agcttttata aaagtcgctc ctaaacttag tgcatctact gcctcagtca atgatgtaca
     8461 tcctggagca taaggaatct gatatctatt gcaaatcttc gctacacctt cattgtaatt
     8521 tggtgcaata ataaattgag ctccatgtaa tatagcatga cgagctgttt cactatccaa
     8581 aactgttcct gctcccacac aaagtatttc accatacttt ttattaagcc cttgaattat
     8641 ttctccagca ttatttaggg tataactcat ttctataact gttataccac cttttataca
     8701 tccttcagca atttcacatg cacggttaag attttcttct cttacaattg caagagcccc
     8761 acattctgac attcttttgg ttatattcat ctttttcata ttatcacctc tatactcatc
     8821 aaaccacttt acttattcat taattttagt catgttttat aatatatata ttaattatat
     8881 accagtgtaa tttccctatt tatattatct attctcacta cttttattaa gtattgtcct
     8941 ataattttca actactcgtt taatttcttc aagtacttcc ccagttggtt cagttacagg
     9001 aagtcgtgca gttccaacat taattccatt caagacaata gttttcttca taacagatgg
     9061 aattgttcca agttttagta tacgtcgaag ttcttctata ctactttgag ctttttgagc
     9121 ttcttccaaa tcccctttca taaagttttc atagatagaa ataccaatct ctgtgaggaa
     9181 gtttgttgtt gctgcaacag ctccttgact accagctttt agactatcca gaataagtga
     9241 atctgagcct gaaaacacac taaaattctc attctgcgtt acttcaatat aagctttcat
     9301 gttgtcaagt ttaccactac tatccttaat tccaacaata ttctttatct ttgataattc
     9361 acgtactgac tctggttcaa tattaattcc agtcttacct ggcatattat acattataat
     9421 aggtaaatta actgaattag caatagtttt gtaatgtaag attaattcct gttgtgttgg
     9481 tgtaacaaag tatggtgtaa taatagataa tgcacttgct ccaatcgctt ccattctctt
     9541 ggataaatct ataacttcac gagttgaatt accacctgtt ccaacaaaaa caggtacacg
     9601 attatttgta tgattaataa caatctttgc aaattcaact ttttcatcat ctgttaacac
     9661 atgacattca ccattagtac ccaaaataaa taatccataa atcccctttt caatcaaata
     9721 atcaattaat ttacatgttg cttgtgaatt aatattttgc ttttcatcaa atggtgttat
     9781 cattgcacat ataatccctt tatatttcat aaaattatat tccctctcta atagactttc
     9841 caagacttcc tgctggatgc catttcaaat actgctgtgt attaagacct ttagcatatt
     9901 gtaatacaac acttaatgat tgtaatacta ttgtttcagc taaaattgaa gcacgaggag
     9961 ctttattaag acaatctcct tcctgtttaa cgcctgcgaa caggaatata tcagaaatat
    10021 tttttaaaaa tgaactctca ttaccactta caccaattat ttttgctcca tttactttta
    10081 gagtcttaag agttgctttt aactcttgag tttcaccact atttgatatt gcaataacaa
    10141 catctccttc aactacttga ccacttgacc catgtactgc ttctgtacca tgcaatatat
    10201 atgctgatgt tccagtagat gaaagcaatg atgaaatata tcctgataca tgtcctggtt
    10261 taccaatacc agtcacatgt acacgtccgt gatttttttc agcttcaaga attaattcgc
    10321 atgctttatt tatagatgtc tcatctattg aggcaataaa ttctgacaat tcattagata
    10381 tattatctaa aaattcagat aatgtatttt ccataagatg tatcctcctt gaaatcatag
    10441 taactatgac tatactttta tataatttag tttaaatagg ttttataaat tgtaaaatat
    10501 tatgtaatat cgaaccaact acgttgtaat caatattagg gaaagttgct ccttctatac
    10561 caagagtccc ataaactgga tatagtataa ctggtaaaaa tgcaagtaat aacccaacaa
    10621 caaatgaccc agcaatagcc cctctccatc ctcctgaagt attgccaaat attgcagctg
    10681 tacctcctga gaagaaacat atgtgagcag ctggaataat tactacagga aatatactag
    10741 gaaatgatgc cataatagca attgctaata gaccagcagc atatgctgat ataaacccaa
    10801 taataactgc atttggtgca tatggaaata ctgttggaac atctagcgca ggacgtgaac
    10861 ctggaatata cttttctgaa atgattacaa atgctgcagt tatttcagct aagaacatac
    10921 gaacacctga catcaatatt gacatacctg ccacaaatgt aaatgcctct ataagcggaa
    10981 aaaccaacca atgtgttgaa ccagcaattt cttgagtaaa tgcttgacca gcttttaatg
    11041 ttgcaatata gaataagatt aacataacta tggatagacc cattaagaag tctctgaata
    11101 ttgataacca cttaggaaat tctatagtct ctgttgtatc atctttatgt tttgaaaata
    11161 aacgcccaat ataacctgat aaaccataac caatcatgtt aaagtgacca atagcttgtt
    11221 cgtcgccacc tgttaattca cgcataaatg gttgacagaa ttgaggtagt gcagcagaca
    11281 taaaccctaa gatagttcca cctattaaaa tagtgattgt attatcaaat ccatgtgctt
    11341 tcatgattaa aactaataca caagaaaaga ataaactatg acctgttgta aagaaaatat
    11401 tcttaaactt tgtaatacgt gcaaatacta aatttaatat gaatccaaga attaatgtac
    11461 atgaaactgc aaaaccatat gtaccttgtg caagtgctgt agctgcttct ggtgaagcta
    11521 taacaccagt aattccaaag ccatgttgga acagtttatt gaaattttga agtgctcctg
    11581 tcatagcaga ggcaccaata ccaaatatca aaaaaccgat aagtgttttt gaagaccctg
    11641 taaatacttc aacggctgat ttcttttgaa gaatcaatcc tatacatgca actacgccaa
    11701 gcaaaacagc aggtgtactc agcaaactaa taataaactc cataaatatt cctccttatc
    11761 atattaaatt tatccctctt ttgaagcaag gaatttttct agtgctgtct ttatttcatt
    11821 acggtcaaca atattgtgaa tcccaattat atcaaacttc aaatttttat tttttaattc
    11881 ttcagcaaca tcaattaatg tttctacagc atctccattg aatccattta gtgaatccaa
    11941 tgaaccatgt tctacttcaa ttggaaagtt attttcctta ataatttgtt gagtctttat
    12001 ttttaacatt aaacttgagc caactcctgc tctacatgcg attaaaattt tatacataat
    12061 taaatcctcc tttataattc gtgtgttttt ataaaatcaa taatttcttt agcctcttta
    12121 gcactatcta aaaccttaaa aaactcatct tcttccaata gttcaactag ctcagacata
    12181 gcacgtaaat ggctattatt gtctattgca cttagacaaa atacatattt tacagggtcg
    12241 ttttcgttat tcccaaactc aataggacta tctaaagtag caatgcctat tgcaatctct
    12301 tttgacccag cttctggtct agcatgtgga agtgcaacat gttttgtaat tactatgtat
    12361 ggtccagatg cttttgctga gttaatcata gcatctacat acccttcagt tactttgtta
    12421 ttttcaacta atacagaagc tgactgtcta atagcttcct cccaattctt agcttttacc
    12481 tttattttta ctagtgtttc attagtaatc tcacttagca taggcccctc tccttcctta
    12541 gatgtaaaat tctctatctg tgtaaaataa gctaatagct cacttgaaag aatactttca
    12601 gaggttacat ttgcatattt ttttattata tccataacct catctatttt aggttgtctt
    12661 aaataaatat cacctaattg aatatacact tcacgtgtaa tcttatattt ttcttttgtg
    12721 gtcataacag ggcttacttt aattactggt acatctgttt ttaaaccctc tgcgttataa
    12781 ttagtagtaa atacgatatc tacaggttca cttatatttt ctaatccaac tgattccgac
    12841 attggtataa agtgtaagtc aggaaataag tttgtaagtt ctgcatgtaa aatagctgaa
    12901 ctaccaacgc catttgaaca aactattaat gctattcttt ttctaaaacc atcaaattct
    12961 tttttatttg aaaaaagtgt tgcaaagtgc attgtaagat atgctaactc ttcttcctga
    13021 atttcttctc caaataatcc gcaaaactct ttcattgcat tacttacaat tctataaact
    13081 aatttatatt cttcttttac tttttcacaa atcggattat agataggtaa tttaaataaa
    13141 agtctatagt aggcaggtct aaaatgagaa tataattgtt taaatatttt ttcttggctt
    13201 atatagtaaa ctccgcttaa atatccaaac tttgtcatca ttttatttac aatttttgaa
    13261 attacatcct tatcttctgt atcttcttca taatttccta ccgatatacc taaaatccaa
    13321 gcactaatat acattatatt ataaggcttt atcttatcct cactcccata tacttctaat
    13381 aattccttag caaatttata ctccttcatt gaactcataa ctgaaatatt aggaatatct
    13441 acattttcct gagtaactat tctagttgaa tacatccttg cttttagaaa gataaatata
    13501 tatataaatt ctattaatct atcttcaaca aaagtaattt catatttttt tattagcttt
    13561 aatataattt ttttagcctc ttcaaaacta tctaaattat attctttaat aaaaatatca
    13621 aatactcttg aacttttctc attagacaaa gtttctataa tgtttttaat aagcactctc
    13681 ctaatttcca tctcattgcc aactatgtaa tatccattta tacgattgtt cttaacttta
    13741 atgttcttct tttcaagctc tggtatcaaa tccttgaaat caagatttac agtagaccta
    13801 cttactttta tagaatttat aaaatgatta attgaaatat cctccatgtt aataaaaagc
    13861 attaaataca tatataaaag tctttctgtc ttactaaaat aataagtatt cttagaatag
    13921 tttttttcta aaatttcttt aattgctttt tttgtttctt ttctaacgat aatatcttta
    13981 tcagctctta aacatataag tggtacattt tttactttaa gcatgtcatt gattttattt
    14041 attctataag taatttgtct ctttgatgaa ttagactcct cttgtaatcc attagttgta
    14101 atcaatgtgc tatttaataa aatctggaga attgatacaa tttctttgtc cactaccatt
    14161 aacccctact ttcttattaa gattagtctt catattatca ttggattctt tcatcacctc
    14221 cacaatactt attttatttg agaattttat cgttttcaat ttcatttaag tataactttt
    14281 gttttagatt gagacaaaaa ttaaacttat ctaaaatagc tactatatta cacttataga
    14341 ttttataaat aaaaaagggt tccttatgta aagaaaccaa aaaaaattta tttaaagaat
    14401 caaagcgata ctaattatgc ataaattacc ataaataaaa ggcacattaa tttaaaataa
    14461 aattaatgtg ccttatttat aattctaata tattaaattt attttttaac ttgtagttta
    14521 atatcctttt tcttcaatta attcatataa ataaagtatt taataataaa tgttccttat
    14581 gactttaata ttttaaacca agaaagttaa agtttctctc atataataaa ttgacactta
    14641 tctatttcat tcttttaagt gtacaataag tcttcttcta gcagtaagag taagtacttc
    14701 ctctacttat cacaaactat aatttttatc tcattggtat atatccacga tactgtgagt
    14761 atgcatccat agttgctata tcacctaaga atactgatgc gatattgaat tgtggtttta
    14821 aatatgggaa ctcctcttct tgtgcccatt ctggtttttt aataaacgac tttgtctctt
    14881 caaccacagc tttataccat tcctcttccc cttcaaacca tagttctgat acacggtcaa
    14941 aagcagttgc tccataatta atcattattt tactactgac aaaacgatta acatagcaac
    15001 agtttgtaaa gtatggcact acctcatcat agaaccattt ttctccttct tctttagaga
    15061 taccatctgg atacttaatc ataaattgcc aacgatagtt tggtccatct tgtacagtac
    15121 gtccttttcc tctaaagtct tcttcccagt ttattggaac aaaagcaaat ataaatggtg
    15181 ggcatccatt atctccacct actgcacgtc ctgcatctcc actttctgca ttttcatgaa
    15241 cccttttatt ttctacatct ggtatacacc cttgccaacg tagaacatcc ataggcatat
    15301 attctgtcat aattctattt gccatctcag gcatatgttc atctacaagc caataatgtt
    15361 ctgttagttg catcttacgt gcaccaaaac gctcaccttc atttggtgta ggatatgctt
    15421 gataaaaggc atatttagta caatatggtc caaaagtgct aatactatct ggaatatggt
    15481 gacgatataa ccagtccatt aacttaggtc tatattcttc cttggcaagt cctacatata
    15541 ttaaacttct cattatttta aattcaaaac ccatagccat tctcctataa tcaaagtata
    15601 ttcacatgct ttcaatttcc ctttttctta tctttactca aaatatatat tgtaaacaaa
    15661 tctacacctg cacccacgta ccatactcct actgccatag ctacagcaat ttgaatacaa
    15721 atatcttgta aattaataat attttcacct gtcataatag ttagacctat agcccaacca
    15781 caaagccatg atggtaggaa aattattttt tcaaataagg atgcaattac aactgcaaaa
    15841 aaacctaata tactaagtgt taaaaataat tctaggtttt gattgaaatt catatatccc
    15901 ataattaaaa tggctaacac tgcccataaa tctccacacc aaaaaccaaa cattattttt
    15961 aatccatctt tttgtttatt accattagtt acatataatc ctgcacagat tagtgctaca
    16021 gctccagttt ttattcctat ataaggtgct aatactgccc ataaaggagg tagtaaagca
    16081 atccccatag acagtgttaa tatttctttt ttctttctat ccatttccta tattgtaatt
    16141 agtagcttcc ctagcttgcc ttatctccaa tagcttaatt gctatttgat ttacaatcat
    16201 agatactgca aaataactta agtataatgg tataaatata acaataaatg ctggagttaa
    16261 tccaatcttg taatatgtaa gtaccaaaaa aataactgct aaaataattt caatttgttt
    16321 aaaacttggc ttttcattca tacaataagc ctcctataac atctaacatc ccttaaaaaa
    16381 gagtcttagt attttaatta ttatcctaat gaaactccag cttgcttttc tttagttaat
    16441 ggctctataa aattataaga tgtttttctt tctttaaatt cttgtgtttt acgaacttct
    16501 gcataatgac cttccatttt cttaatatca tcaccatgta taaacttacc tggtaaagta
    16561 tcatgagcat gacctaattg ctcttctgga aaactaaata tagtttttcc acttatagta
    16621 tctaactctc caactatacc acatagagca caaattgcct ttttagtacc tgactcaagg
    16681 tagaataatc tattgtggca atgtggacat acaccttctt ctccttgata tgaagctttt
    16741 tcaaaatctt ttgctgcttc aactatattt aaaccaattt gatgaacttg aacaagcctt
    16801 gtatcatcca ttactaattc ctttgcccat gggaaccaag cattatctat aactttccac
    16861 attggtatga gagctagcat agcatgttca cattgaactc ttgtacccca gtcagaacct
    16921 cctattccca taaatgatat tactttatca ttaagcattc ttgagtctgg aatttttcct
    16981 cctatagctt ctgcaacttt tgttcctata atgttatttc ctctatctgc tctgggtcca
    17041 aaacggtcca ttatagtatg gaataatcct gatgcccctt cttcaaaaat agggtctgaa
    17101 actactatac catctgcatc tttcattttg tcaagcaacc attcaaaatc atcttttaat
    17161 acacacatac tacctttccc ggataataaa gttttaacgc acgcacaaca tccagtacaa
    17221 tgtttaatgt taatagatga catttgaata aattctactt cagcccctgc ttctttggca
    17281 cctttaagtg caactttaca cattgtgtca ttatttccat tttttgttcc aaaagaaata
    17341 cctagtattt tcattttcaa ctctccctct ttagtattta tttaatcttc tttcattttt
    17401 aatatataga atccatcatc taaagcatct atgatgatgt agcctctatc atcaacgata
    17461 agatcttctg ttactgccaa gcgtggtcct gggaatcctg ggaaataaga ttcttctggt
    17521 gttttatttg gatttggtgg tataaaatat gctagctctt tgatataata tgggtctgat
    17581 acatcataaa cccttagccc tgcatggaaa taacagcaat atactctatc tcctctttgc
    17641 tctaaccatg gcttattatc cattggttca tgaagattat gtggaccaaa tggccctgct
    17701 tttcctaatc ctgcaacatt aaagttagga taagggaaat cttttggaac ttcaggatat
    17761 gggaattgag caattaatgt tggttttgtt gggtcactaa catcaaccat atgtatatta
    17821 ttcatagctt gaacatctgt aatgttatct ggtttaaaga actggaatct ttctccctca
    17881 ttttgaacaa cgactaaatc tcttcctggc aatggtaatg ctgtatgagt tcttgcacct
    17941 gcaagtctac tagaaaatgc aggcataaat ggcaattcac ctaagcatct tggtcttgtt
    18001 aaatcttcaa catctaatac aactaaacca gctcctccat aaccacaata tgcttttccg
    18061 tctcttacaa atggaggtcc atgaagccat cctttatcca taaattctgg gcaatgaggt
    18121 gctcctgcat caaaagttct atttggatat ccatctgcat attggtctgg tctccaccat
    18181 ttacctattt ccactggatt attaggattt attatatcta tgaccctata aatcagacct
    18241 tcaaagccaa cacaatcact tgataaatgt acatatcttc caccattgta catgaatcta
    18301 tgaacaccca ttacatgctt taagccacaa tcccaatatc ctaaaaactt aggatttaaa
    18361 gggtcttctt ttaaactgta tattctaatt cctgcttcac acttaatatt tgctaattta
    18421 gcttggtcaa caagtgctcc tggtccactt ccacaactca ttgctactat cattaaatca
    18481 tctgctattt gaatttttgg agttgttgtt gtaggatact ctttagggtc taacatttga
    18541 aaatgtttta taaattgtgg attataaggg tctgtaatat cgctaatcat tactccattt
    18601 tttttatttc ctccaaaaca gcatccatat aaatagtatt tttcatctct tttgtacaga
    18661 gccatttgaa acattccaca agttccatct aagttattaa aggaacaaac ttcaagattt
    18721 ttaatatatc cgttatttcc gttgcctttt gcataaaatt tatcttccat tttttctccc
    18781 ttcttattta aagtattatc aattgatata ctttcatttt atgagacaaa cttacttaaa
    18841 tccaatatcg ttttcattgg aatatataag ttattcttat taataaattt ataaaatcta
    18901 atgtaaagat atacttaaaa catatatttt tatacaaata ttttctaata ttctttaaaa
    18961 gtaaattttc ttatactata ataagcttta attatagata aataaggttt acaaacaatc
    19021 aagtttaaat aggggtgatt atttgaatac taaacaatta gaatatttta tatctgtagc
    19081 agaaaatcta agttttacca aaacagcaga aaaattttat atttctcaaa ctgctgttac
    19141 acagcaaata aaagctttag aagaacaaat aaatgtaact ctatttacaa gaagtaaacg
    19201 tcatgttgaa ctaacaccag ccggaaaagt ttttttatct gaggcacgta ctataataaa
    19261 aaatatcaat gatgctattg caaaaacaca acaatttgca catggttttt ttggaacttt
    19321 gagcattgga accttaatag gatatgaaaa aaacaaactt caacaatact taaaagaatt
    19381 ttctaataca taccctaata tatctatgga tatttgtact aatgaaataa ccgaactttt
    19441 aaacttagta aaaaataatt ctatggacct cgcttttgtt attaatccag aaaatcaacc
    19501 attaaaagat tttgaatata aaactgtaga aagatattct ttggtagctc ttttacctcc
    19561 tggtcatcca ctttacaatg aggattctat cgatttaatt gaattacaac atgataaatt
    19621 tatttttgta aaagaaacag gggatgagta tggacaaaag tcaatgatac aaaatagata
    19681 tcgtgaagcg gggtttgtac ctaatgtggt acaacgttca aataatctta atactattga
    19741 atcattggta tcttctaaca tgggaatatc cattttacct tcattttgtg ttacagatac
    19801 aatgatagaa cgtgatattg caatagtacc tataaaagaa gtagaaaata gaattgaagt
    19861 tgtggttgta tggaataaaa aaaatacaaa tccagcatta gaaaagttca tttctataat
    19921 ataaaattaa tcaatattaa ctataaaaat agcattaacc atttttatat ggttaatgct
    19981 attttctact attttattat ctaactttta atttaatatt tataatcgtt aaaaataact
    20041 ctgtttaaca aatataagca agtcaaaagt gtgtctaaaa aagataaaaa ttttattata
    20101 tacaaatgta ttatctcaat atatctattt tttcattgtt catcagtcta agtacatgac
    20161 ctgtgcttac taaagaatta tccccataag ttgtatgagc taacaccgaa ctagcaaccc
    20221 caaactcaac tatatcttca ctcttcatat tttccaaata tgaatatata attcctgcca
    20281 catatccatc cccagcacca actctgtcat atatttctaa cttatactcc ctactttcaa
    20341 aataaccatc atcatttgat atatatccct ttataaatct attattcata tcatcaaatc
    20401 ttctcatagt tccagctaca atatctatat catactcttt tttaatcaat tctgaactag
    20461 attttatatt tagactatct atattaagta tcttatttat atctctataa ctagcaaaca
    20521 gcccatgaca atatggtagt attttcttat aatcatttac taaatcatca taagtccttt
    20581 ctggatttaa tgattgtcta aaattaaaat caaagaatac ttttatcccc ttttcataag
    20641 attttctagc tagttctaaa gcaatctttt taactgaatc tgtaagttgc atagcaatac
    20701 cacaaatatg gactatatcc atttcttcta aacatttatc aatatcataa tcttgtaatt
    20761 tactctcaca aaatgaacta tttaacctat tcaaataagt cacttcagaa ggtctttctc
    20821 catacccatt ttcaagaaaa tacactccta tatgattccc tccatatgtg ataaagtcat
    20881 cttttactcc aagctttctt atatttgcac ttgatgcttt acctacatta ttattaggca
    20941 actttgtcaa catataggta ttgtatccaa agtttttaag tccagataat atatttaatc
    21001 ccgtgcctgt aattatataa tttagttcat tggtttgtgt taagaacttg tagtcaggta
    21061 ctgaaagtct catcattatt tcaccaaaag ctaatatatt cataaatcta tcccaataat
    21121 tctttcatta ttttataaag tactcctata tcttccactt tcgtatcacc tgtttcttta
    21181 tcaattattg atgtataaac atgaggtata actttttcta caccagcatc aagtgcaatt
    21241 tgaactatct ctttgaagtt atctaagtct ataccccctg ttggttctaa ataaaaatta
    21301 tgctttgcac aagcttttgc aacttctata tattcatcct ttgttttaag ccctcccatt
    21361 ggaaaaaact taattgagct tcctcccata tctttcatca tagctattgc tgtttctata
    21421 ggaactatac catctttttc ttttgaactt aatggacctg tggatatttt tacaaatcct
    21481 ggtttaccag ttggagaaac taatccattt attatactat cattttgacc aagcaatgct
    21541 ctagatgtag cgacacctgt aaacacttga tttatatgtt gtggctgaag ttctttagaa
    21601 atctgagata ccatatttga ctgatttggg tctccagctc caagacccac agatattgca
    21661 ttatctattt cctttgagta ttttttcata tcttcaatag ctgcttctac agtgctataa
    21721 ttctttgata gtactcctac tagtacatgt ccatcacagg catcatatac ttctttagca
    21781 ttttttatgc ttcctgcaag aacatttaaa caaactctat ttttataata ttgtggtata
    21841 caattcatat ttatcccctc cattggtaaa attcaattta ttaaatcatt ataatgtcac
    21901 ttactctaca ctctaacttt ctactatatt ttttatccta ttataaatat tatctaaatc
    21961 acttccagtt acacttctta tatcaatctc aatcttgcct tcattagcct tatagtctct
    22021 agtgtatata gctatctctc cacttctaag ttcatttata atttgttgtg ttgacttatt
    22081 tattatatct tcattaaagt taatttctcc acgaagtatt tctctacctg ctgaatctct
    22141 agttatacta gcactcactc cctttatagt atttatcttg tctataaatg gatttagctt
    22201 gtcctccatt tcttttaaag ttactttatc attacttata tatctctcaa ttgcacatgt
    22261 tagacctaca atattttctt ttcctatttt catagctctt cctataccct ttgattgaag
    22321 ctttacatta tctatgtatt ttttcttacc tattactaac cccgagctag gaccttctat
    22381 agccttagca ccactgtata tcactaggtc tgctcccaat tcataatata catttaaatc
    22441 ttcttctgct gctgcatcta ctatcaaagg taaattatat tctttagcaa cttgtactgc
    22501 atctaataca cttagcatac tcttttgaac acaatgatga gatttaatat acataattgc
    22561 tgctgtattt tcatttatct ctcttattat atgttccttg ttacacatat tagcatagcc
    22621 ggcctcaaca acttctcctc ctccaagtct tatcattact tctactggaa ctccataatc
    22681 aacattatgc ccctttggaa ttattatttc ccttttaact tgtttcttag gattatttac
    22741 attgtaaact agactcatat catcttttgc tatcattgct cccacactct gtgcaatccc
    22801 agcagatgca ctagaaacta ttagtgcact ttcacaccct aataaatttg ctatatattc
    22861 acctgtttta tttacaagtt cttctattat aaggaaattt ttacaccctt cacctattgc
    22921 ttcaataact tccttatcta ctgttgatac tcctagtata gtcattttgc ctgaagcatt
    22981 tataacacgt cttacagagt atttttcaaa tatactactc atacaatagt ttctcccttc
    23041 tactcattct cttctataga gttgcctata tttaaatatt ctccattgac tactactgct
    23101 cttggcttta tagacgtact tgtgattact gacttattca aagaatcttg aagttctttt
    23161 tcttcattaa ccacatcaaa tatagttaaa tcagcatcat atccttcttt tattaaacct
    23221 ttatttttta aacttattgc atctgctgca tttttagtaa ctttatctat tatatcttca
    23281 agtgaatagc ccatatatat aaacttttcc attgttgtag atagattata cactggacca
    23341 tttattctat ttttaatata tatatctgta cttatagtgt ctggaaaaat tcctgcatcc
    23401 ttagatttca ttgcaatatc catactaaaa ctttcggttc catgacctac atcaagaatt
    23461 atacctcttt ctttagcttc tatgatttcc tttttaacct cattgcctct aagtattcca
    23521 tttggctttc cattatatat atgggttaat atatctcctt tttccatata atcaaatata
    23581 tcctctatcg ttggaggaga actcccaaag tgaaccatca taggtagatt aagttcattt
    23641 tttattcttt tagctacttt taaaggttca acatcatttg aaattacaac acttttactc
    23701 attctagctt tgattccaac aacaaaatct ttgtattttc taactgcatt tttaagttca
    23761 tattcatcaa tattcatcat atttgaaagt tcatcttgag atgttattcc ctgcttagca
    23821 atatttatta aagcatatac attggtctta tactttttag tatcttcata aaattcacct
    23881 atatctaaag ctcctgttgt tcctgcatct actacagtag taactccact ttttattcca
    23941 acctcatctg gataatcctt ataaaggtct agtctttcgt agcagtgtgt atgtatatct
    24001 atccaacccg cactaataaa tttctctcca tctaagttta taatttgttt tgcctgatac
    24061 ttagaatcat ctatatgatt ttttacttct attattttat cgtctaatat agctatatca
    24121 attatacttt tatctactgt ttttccacat tttattaata tgtctatctt cactttactc
    24181 tccctctaaa ataatcttaa atttactcca aggttcaata aagtctaaaa gtattatttc
    24241 gtcttccttt attctaccca ccttattttt tctttcatct gtacaatctt gaagtatcaa
    24301 ctgtaattcc cctttatact ttccaaagtc atcattacca atcactacat ctcctctttt
    24361 aaagatgttc ttattatcat gtttagggaa attttcttta gcatattttt ttcttacttc
    24421 tgtagatctt gctacctgat tagttatatc tcctcttcta aagtgttgct catttaccat
    24481 tattatcttt tctacttcag aagttttttc tttagctact acattaaata caacttggta
    24541 ttcatttact tcagacattc tttttaattc atcttcactt gcatatgcat ttcctacaat
    24601 tacatcatca atcaatcctg ttgcgaataa gtgcttagtt gctgtagcaa tatctaagtt
    24661 tctatgcatt tcaagcgtac acagtccatc atttatattc caaggtccga ttgttgcgta
    24721 ttttgaattg acaaaagcag cagttcttat tccatacttt ttaaatctat ttgagcatct
    24781 tacaaaaaaa tcatatggca agcctgtacc cctttgagga taaaaattgt gacaaccata
    24841 tatattattt acatttggct tgtaagtaat tatattatcc aagtatgcta catcattact
    24901 catatttagt tccactttta aatcatatgg attgaaagaa atcatagctt ctttacttcc
    24961 atcatatcca tagtcaagcc ttattccact agcatttaac tcactaaaga atgttaaatc
    25021 atcataagat atttgtaata tatcaaatat gtttggagct atatcaagga ttacttcaaa
    25081 tcctaattct cttgcatagg ttataatctc tttaaaatgt tttttaactt cttccttagg
    25141 tcttgtaatt gaaagcatgc acataaatat tcttttaaat ccgtattttg aaaccatttt
    25201 tatatatgcc ttatcatctt ccatgttact atgctctgga tatactgata ttcctaatct
    25261 tctttccatc tttctcctcc tcaaattaca ttatttaaaa tatataaaat taaaggcaaa
    25321 ggtcattcct cctaccttca cccttaataa cttataagct ttttctatat tttatatagt
    25381 acaaaacctc tatatctatg tatttttata aattttcact catttcttct tgttctcttt
    25441 gtaattgttg tttttcaaaa actttgaaga atggataata aacagcaaat gatatcacaa
    25501 agttgattaa tactaagata cctgctagta tactccagtt agtactcatt actgctgcta
    25561 ttggtgctgg cattgcaaat ggtaatcttg ctaccatcat aggtataacc cctgtaatag
    25621 taagaacata tgatagaatt gtcattatta taggtccaat tataaatggt atccctagta
    25681 ttggattcat aactattggt gctccaaata ttataggttc atttatgttg aataaccctg
    25741 gtaaaaatcc taatctacct aaacttttta gatattgtga cttagaaaac ataaacatta
    25801 caactaatgc aagagtagcc cctgctcccc ctatccatac ataccactgt aaaaattgtt
    25861 ctgtaaatat gtttggcatt gcatgagctg atactccttc tgtgaatgca gttatatttt
    25921 ctgctataga catgtcccat ataggtctta ttactggccc cattatagct ggaccatgta
    25981 tcccaagtac ccagaataaa gttattagga atactgtcag tagcccacca cctaaactat
    26041 ttcctgataa gaatcctttt aaaggtgata acatagcgct tagtgcaaca cttatatcaa
    26101 atcctaaaat atgtcttatt acccagaata acattaaaat tactgctgtc ggtaataatg
    26161 cagtaaatga atttgaaacc tctactggaa ctcctgctgg catcttaatt gtaatattct
    26221 tttccttcat aaaacgatat atttcaactg taattaatga tgttacgata gctccaaata
    26281 atgatggcga attaagtttt gccatatcca tccatctacc agcttctata actccaccta
    26341 ctggttcaac tacttgagtt ggaactattg tagaaactaa aaatgccata actgcaagca
    26401 taccaacacc tgttgtatct aaatcgtagc ttttagcaag tgatgatgct atcccaaaag
    26461 tagcatatag agataaaaat cctacagtat atctaaatgg tatatctaat gttgctttaa
    26521 atggagctat aaaatccata taagcatcaa taggtatgtt taataatatt gtgaaaaatg
    26581 accccactat tgttagtggt aatgttgcta ttatacctgc tcttacagct ctcatatgtc
    26641 tctgattact tatcttcgct gctacaggca taatcttgcg ctctaaaaaa cttgcgaatt
    26701 tttccatagt tatccccctg tattattgat attatccttt atttataatg caataacgat
    26761 ttcataacat aaactaattt ttatgtaatt gataatatca cttatttatt aaaaatatta
    26821 taacattata atgtttggtt gtaaacatta taatgttata atatttttcg tttttacaac
    26881 taaacaaaaa tcctgagcat attatcattt caataaactc aggattctta ttatatttcg
    26941 tatttaatta cgtagttttg tacttctgta ttatatcttg caaatgatag ctcaactata
    27001 ttatcattct catcataggt ctttcttttt cttcctaata atatgtcttt agctagattt
    27061 agagcctttg atacctctag ttctgattct tctataaaaa attcatcttc aaatctagtt
    27121 atatttatat ttctcctata taagtacata tataaagaac tttcattatt aaaactttca
    27181 acagttagat tttcatcaaa aggaatataa tgagtaaaaa atatatatgg cttactatct
    27241 aagtaataca ttctagtaat cttgctacat ttttctccaa atatactaaa tacttgtgaa
    27301 tttgtatcat ttattatttt ttcaattgaa atagtctttt tagataactt atgaccttta
    27361 gataaaagta tacttgaaaa agactctgct tttgatagct tattaaaaag actattactt
    27421 attacttttg tcccttttcc actttttttc tctacatacc cttctgattc taatatctca
    27481 atagcttttc taatagtaat tttactgact ccaaattctt gctctaattc tctttctgtt
    27541 ggaatatttt catcaatttt atattcgtta tttaatattt tttgctttaa tatatctaca
    27601 acttgtatat ataatggttg atttcttaac atgattcacc tcttaaa
//
LOCUS       DAGQWR010000046        27113 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.046, whole genome shotgun sequence.
ACCESSION   DAGQWR010000046 DAGQWR010000000
VERSION     DAGQWR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27113
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.046"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(202..963)
                     /locus_tag="MIW95_001681"
     CDS             complement(202..963)
                     /locus_tag="MIW95_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY3151010.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            1182..1658
                     /locus_tag="MIW95_001682"
     CDS             1182..1658
                     /locus_tag="MIW95_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBY3151011.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1756..1908
                     /locus_tag="MIW95_001683"
     CDS             1756..1908
                     /locus_tag="MIW95_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBY3151012.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(2068..2256)
                     /locus_tag="MIW95_001684"
     CDS             complement(2068..2256)
                     /locus_tag="MIW95_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBY3151013.1"
                     /translation="MAKVCSVCDKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            2617..2967
                     /locus_tag="MIW95_001685"
     CDS             2617..2967
                     /locus_tag="MIW95_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBY3151014.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            2986..4629
                     /locus_tag="MIW95_001686"
     CDS             2986..4629
                     /locus_tag="MIW95_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBY3151015.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            4710..6767
                     /gene="recG"
                     /locus_tag="MIW95_001687"
     CDS             4710..6767
                     /gene="recG"
                     /locus_tag="MIW95_001687"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBY3151016.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIRNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMERFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            6826..7380
                     /gene="rsmD"
                     /locus_tag="MIW95_001688"
     CDS             6826..7380
                     /gene="rsmD"
                     /locus_tag="MIW95_001688"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBY3151017.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNVKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            7382..7879
                     /gene="coaD"
                     /locus_tag="MIW95_001689"
     CDS             7382..7879
                     /gene="coaD"
                     /locus_tag="MIW95_001689"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBY3151018.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            7883..8407
                     /locus_tag="MIW95_001690"
     CDS             7883..8407
                     /locus_tag="MIW95_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151019.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            8751..10670
                     /locus_tag="MIW95_001691"
     CDS             8751..10670
                     /locus_tag="MIW95_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY3151020.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQERFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISKEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            10683..10970
                     /locus_tag="MIW95_001692"
     CDS             10683..10970
                     /locus_tag="MIW95_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3151021.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            11103..12362
                     /locus_tag="MIW95_001693"
     CDS             11103..12362
                     /locus_tag="MIW95_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBY3151022.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISKFIGSLFGGIL"
     gene            12365..13105
                     /locus_tag="MIW95_001694"
     CDS             12365..13105
                     /locus_tag="MIW95_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBY3151023.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            13428..14738
                     /locus_tag="MIW95_001695"
     CDS             13428..14738
                     /locus_tag="MIW95_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBY3151024.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            14836..15753
                     /locus_tag="MIW95_001696"
     CDS             14836..15753
                     /locus_tag="MIW95_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBY3151025.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            15769..16578
                     /locus_tag="MIW95_001697"
     CDS             15769..16578
                     /locus_tag="MIW95_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBY3151026.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            16635..18383
                     /locus_tag="MIW95_001698"
     CDS             16635..18383
                     /locus_tag="MIW95_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBY3151027.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKEKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSTISWKGLENRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLNIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDKSKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMSKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDENDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIENLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            18646..19668
                     /locus_tag="MIW95_001699"
     CDS             18646..19668
                     /locus_tag="MIW95_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBY3151028.1"
                     /translation="MFGGINIKRIGIIIMFFLSIAFIGFYLAIRGNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEEFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNVPKDSNIVV
                     SFHYYEPNDFAFQGNIYHNGFEHLSNITWEGTNEQMDYLKKRFDIVENWANKNKVKIF
                     LGEFGVTKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            19673..21979
                     /locus_tag="MIW95_001700"
     CDS             19673..21979
                     /locus_tag="MIW95_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBY3151029.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPVISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKNKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKIRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKNIYPKEIALEINENIVLECDLCEGKIDERGLLYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTIPVSNPSEKKEKSIDEFEPMEYI"
     gene            21951..23066
                     /locus_tag="MIW95_001701"
     CDS             21951..23066
                     /locus_tag="MIW95_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151030.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSAKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSRITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQKINLSIQSKNMLEFRLKEGSEIFLEDKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEKSNVVLDSSMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     SFLHNIKITGNEEVQIPVLGQSISQYEVLGLKRDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAEPGQIILQVNVDDKFKIGRKIALTESWSINKKDKDGVPYTLVSPEQSPTVQYMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            23084..24502
                     /locus_tag="MIW95_001702"
     CDS             23084..24502
                     /locus_tag="MIW95_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBY3151031.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCVYNIGVLFIFKYLAFAL
                     RNISELINTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTMQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMLGG
                     NGVIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            24517..25725
                     /locus_tag="MIW95_001703"
     CDS             24517..25725
                     /locus_tag="MIW95_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151032.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNICYSGKDMIQEIKNSVAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSEEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKENYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCK"
     gene            complement(25806..>27113)
                     /locus_tag="MIW95_001704"
     CDS             complement(25806..>27113)
                     /locus_tag="MIW95_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBY3151033.1"
                     /translation="FFIVRQFEKKKIKFSLRTIYATVLGLILGIIIQAFAGFPNDTTQ
                     VTWLQEVSKWYSLFGSGFMDLLKMLVVPLVFLSIIKVIINMKDNNLGTLTFKSLGMLL
                     ATTAIAAIVGIIVANIMKLGVGANMPTLVENQELKEINSLVDTLRGLLPSNPVMAMAN
                     GNIVATIIFATFIATSIKRLSKKHFETIKPFIDFIEASYKIIVSVSMTVIKFMPYAVV
                     ALLANTITSQGISSIVSVLQFILALYISVAILFTIHLIFITLNGLNPITYIRNSTEAL
                     LLAFTSRSSLGTLPVTIESLVQNHGVDEGVSSFTASLGANMGMNGCAGIYPALMAVTL
                     ANMAGVNMDMSLYVMLLIVITISSFGIAGLPGTATMAVSVVISGVGLGSYFPLVGGIL
                     AIDPILDMGRTMINVNGAMVSSITVGKSLGKIDKEVFGKKNIS"
BASE COUNT        10511 a         2960 c         4747 g         8895 t
ORIGIN      
        1 aatagtataa gtaagttttg tataatttgt tattattttg tattgattat aattattata
       61 caataaagat tctgtctcaa aatagagaga gaaacttgtt tttcattgat aaaatgataa
      121 aaatatattt gatttcatac aaaaaagagt gtctcatgaa ctaaaaagtt cattttgaga
      181 caccaccttt attattttat attatttatc tcttgtatat attactatag catcccttaa
      241 aaacttagct aatccaggtt gttctctatc ataatatgat gtaaaacgtt catcatatac
      301 atacatctgt gctagagagg aatgagcttc tttagagtat gtgttccaag tgtaactcaa
      361 ccatttatga tgtaattttg caacttcctg agccagttca ctagaggcat caccagtttt
      421 aaaagctttt ttaagtttac taattatttc gatatttagg ttttgccaat tttcataatc
      481 ttgcttagac atatttttaa gctttttatt tgatgtatct ataatatctt taccatattt
      541 ctctctaatt tcagttccat aatttttttc attttcatca atcatctttt ctttaaaacc
      601 ttcaaatttt tctatatctg tcatagtgta ttttccctcc ttcagagcta tagttttagt
      661 tacattttct attagtaaat caatttgctt tcttttggct agtaatttat tatggtgttc
      721 ttttaacgca cttaaactat cgaagtttgg tgaatttata atgtttttta tattttctaa
      781 gttaattcct aattctctat aaaaaagtat ttgttgaagt ttattaactt cattttgacc
      841 atatatacga taacctgaag aatttatttt aagtggctta agaagaccta tttcatcata
      901 atatctaagt gttctagtac ttataccagc tagtttactt aatttttgaa ctgtatattc
      961 cataagtaat acctcctata aattaaatat acacttttac gcaacgtaaa tgtcaatata
     1021 ttaaaatata aaaatttata tcttttaata aaaatataaa aaaatatctt tactggatat
     1081 tttacacaca actgtcaata ctcactaatg aaaattaaaa aataaaattt atagactatc
     1141 tttactaaaa tatagaaggt ctatagccta ggaggaactg tatgaattta aaaagaagca
     1201 atgaaaaacg agaagagtac agaagaatgt attcagataa agaaagtttt ttaagtttga
     1261 ttcaaaattt tacaagtgtc tcaatagcaa aagaattaac tcttaaaaat atagagttag
     1321 aaacatcttt tatatgtgaa tataaaggaa aagaagttga tataatttat aaggtatttt
     1381 ctaaaagtgg caaagtatca cattatatag tgttagaatt tcaaacagaa atggatactg
     1441 aaattgtccc aagattgaaa tcgtatagag aacaaatttg gaaaagtttt attatgaaaa
     1501 aaagccttga agaaatagaa gataaaaatt ttaaacttcc taaagttata ccagtggttt
     1561 tatatagtgg acctgaaagg ttaagtagaa aaaaaggaat tttggatatt gtagaatcta
     1621 tatcggagtt cagtaataat tctagtatga aaaattaaaa ttacataatg tgtttattgg
     1681 tctaaaatgg aaaactgtcg cataaacttg aatatgaaaa tatttataga tacaggttct
     1741 atgtggaggt gttccatgag aaataacaag tctaaggtat tgctaggaat actttgtcta
     1801 gcaacaggaa tggctgtaat tctgtcaatg ttgcttcctg gctggatttg gtcagcactt
     1861 accgcattaa tacttattgg atgtggagca ttattatttt tttgttaaaa tagttttaac
     1921 tataaagtgt ggggtgagta ttgatgaaag tagtaacaat aaaattacca aaatggattt
     1981 caagtataat cctaaaattt atgagaaaga gtaaaaatga ttaaattaaa aagcctactt
     2041 acggaagtag gcttttaaac gtggttacta agctctttca attttaccag aacgtaaaca
     2101 tctagtacaa acttttactc tcttaggagc tccatctata atcgctctta cgcttcttaa
     2161 gttagctgac catgttcttt tattatgttt atttgagtgt gatacttggt ttccagaaac
     2221 cttaccttta tcacatacgc tacatacttt tgccatctac gcacacctcc ttaaaatgat
     2281 aactatttaa agactaaaaa catatttata ttatcataaa taacaaaaat ttgcaataga
     2341 aatatatatt tgccttacac atattgaaga atttcttatt atcatataaa attatatata
     2401 taattttata taaagtaaat atatttggaa aataattatt atttttcttt gaatatatat
     2461 actatagaag aaattttatc caaaaacttt tctaggttta aattatacca tatttatagt
     2521 atgatatgat tataatttta tataataaat aagtaaaaat agtaaaaaat aaattaagaa
     2581 atgtttttaa taaaaataag cttaaggggg tcaaccatga gtgcaaaggt aacgaatcaa
     2641 tatggaaata tagaaataga taatcaagta atagctcaag tcgtatatag agctgcaatg
     2701 gaaagttatg gcttagtagg tttagcatat aagtctaaag gaatagtaga gctattaaaa
     2761 ggcgagaacg caactaaagg tgtaagagtt gaggaagtag aagaagatgc catagcaatc
     2821 gaactttatg ttataataca atatggtaca aatatatcta ctgttgccaa taatataata
     2881 gatagagtaa agtatgtagt tgaaaaaatg actggcgtaa gagttactag aattgatatt
     2941 aatgtacaag gaataagagt taagtaattt cataggagga aacaaatgat tcaatatata
     3001 gatggtaaaa ggttaaggga gatgtttatt tcaggtgcaa acaatctcca aaacaataaa
     3061 gagttagttg acaagttaaa tgtattccct gtaccagatg gagatacagg aactaatatg
     3121 tcattaacaa tatcttatgc cttaaaagaa ttagcaaaag ttgaaaatga taatatatca
     3181 gatatcggaa aagcattatc taaaggttct ttaatgggag ctagaggaaa ctcaggagtt
     3241 atactttcac aaataatcag aggtatagct aaatctatag aaggaaaaag taaattatca
     3301 acagaagatt tagcaaaagc ttttaaaaat ggttcagaca cagcatataa agctgtaata
     3361 aaaccaattg aaggaactat acttactgta gttagagaaa gtggagagtt tgcaataaaa
     3421 actgcgaaaa aagagaaaga tgtagtaaag tttttaagta tgcttgtgaa ggaatcaaat
     3481 tcttcattag aaagaactcc agaccttcta aagaatctaa aagaagctgg tgtagttgac
     3541 tctggtggaa agggtcttgt acttatatat gaaggaatgt tagcttctat aaaaggcgat
     3601 aacatagaaa taaaaaatgt tgacctagat actaatattt caacaagtat ggattttgca
     3661 aagagtacta ctagcacaga tgatataaaa tattgctatt gtacagaatt tattttagaa
     3721 agttctaagg tagaagatac taaaataaga gacataatga tggcttatgg tgatagtcta
     3781 gcagtagttg gagatgatgg agtcataaag gtacatgtac acactaatga cccaggaaat
     3841 gtacttcaag aagctctaaa gtacggtcaa ttattaacta taaaaataga aaatatgaag
     3901 ttacaacatg aaaatacatt actggatgta gaagaaaaga aagaaaatga tagtgaacct
     3961 ttagaagaag agaaagagtt tgggtttata gctacttcta tgggtgaagg tttggcaaat
     4021 atatttaaag atttcggagt tgaccatata atagaaggtg gtcaaactat gaatccaagt
     4081 actgaagatt ttatgaatgc tataaagcat ataaatgcta aaaatatatt tattttccca
     4141 aataacagca atataataat ggctgctaat caggcaaaag aattgagtga taaaaatata
     4201 atagttatac ctacaaaaaa tacgcctcaa ggatttgctg cattagtaac ttttaatgga
     4261 gaattaagcg aagatgaaaa taaagaagct atgatgaatg ctttaaactc agttaaatca
     4321 ggtcaagtaa cttttgctgt tagggataca gtgatgaatg aaattgatgt taaagaaggt
     4381 aatataattg gaattgcaga aggaaattta ttatcagcag gagattatgt ggatgaagta
     4441 acatcaaacc ttattgaaaa gttggttgat gaagatactg ctataataac attattcttt
     4501 ggtgaagatg ttacagaatc tcaagcaaat gaactacgta cttctttaga agaaaagttt
     4561 gaagatgtag atgtagagtt atattatgga ggacaacctc tttattatta cttaatttca
     4621 gttgaataat gattataaga acctgtatga ccaagtagct tttataaaat tggttgtaca
     4681 ggatttttgt gtagtaggtg atatatttgt tggatttata taaagatgtt caatatgtta
     4741 agggaatagg acctaaaaaa gcagataaat taaataaact gggtatattt acactaaaag
     4801 acctattgta ttattttcca agacaatttg aagatagaaa taatctaaag aaaatagcac
     4861 aattagaaga tggtgagaaa gttacgataa aagcagtaat atcaagtata aatacattta
     4921 gcccaaaaga aggtatgact ttaacaaaga ttgatgtaaa agatgagaca ggctcggcta
     4981 aattagtgtt ttttaataaa agttatataa aaaatacttt tcgtccagga gattcaatat
     5041 tagtttttgg gaaggttaag aaaaaattta ataacttaga acttacatca tgtgaattag
     5101 aatatcttac taattcacct aagaatacat gtagatttat gcctgtgtac cagcttacat
     5161 acggtgttac aaataaagaa attatgagta taattagaac agtacttgaa gataaagaat
     5221 taattataca agaatatatg ccacaaagaa ttatagaaaa atataggcta tgtagtatag
     5281 attttgcagt tagaaacata cattcaccaa gtagtaagga atcattaaaa atagctttat
     5341 atagaattgt atttgaagaa ttacttatac tacaactagg gttgtttgta tttaaaagtg
     5401 gcagaaataa agaagatgga ataaagtttg aaacaagtaa agacttaaaa aaaataataa
     5461 gtgctttacc atttaaactg actaaagctc aaaacagggc attagatgaa attatacaag
     5521 atatgaattt agagaaaata atgaatagat tggtacaagg tgatgtaggg agtggtaaaa
     5581 cagtagtagc tctcttagca cttgcaaatt gtgttttaaa tggttatcag ggcgctttga
     5641 tggcacctac agagatatta gcagggcaac attacatttc acttactgag tctctaaaag
     5701 attttggaat aaatgtaggt ctattaatag gtagtcttac aaaaaaacaa aaggatacag
     5761 tattggaaca aataaggaat aatgagattg atatattaat aggtacacat gcactaattg
     5821 aagataaggt tgaatttaat aatattggtt tagttataac agatgaacag catagatttg
     5881 gtgttatgca aaggagcaaa ctttcattga aaggagcaaa tcctgatata ttagtaatga
     5941 cagcaacacc aataccaaga actttagcac ttatactcta tggagattta gatatttcta
     6001 ttatagatga acttcctcca ggaagacagc caatagaaac aattgctatt gaaaaaagta
     6061 agcgagatag agcatataat aaccttgtaa gaagagaggt tgaatcagga agacaagtgt
     6121 acattgtatg tcctttggta gaagaaagtg aagctataga agctaaatca gcagttgaac
     6181 ttgtagagga attaagagct gaatatttcc acgatttaag gcttggattg cttcatggta
     6241 aaatgaaatc gagtgaaaaa gacgaagtaa tggaacgttt taaaaataaa gaaatagata
     6301 ttttagtttc aacaacagtt atagaagttg gtgtcaatgt cccaaatgca acattaatga
     6361 taatagaaaa tgcagaaaga tttggacttg cacaattaca tcagcttaga gggcgagttg
     6421 gaagaggtag tcataaatcg tattgtgtac taatttatga ttctaaaaca gatgtatgta
     6481 gacaaagaat ggctataatg gaagagacca atgatggttt taagatttca gagaaggatt
     6541 tagaaataag aggacctgga gaattttttg ggacaaggca acatggtctt ccagaactta
     6601 aagtagcaaa cttatttaaa catataaaaa tattgaagtt agcacaacaa gaagctcgat
     6661 atatattggg tgaagataat aatctacaat tgaaagaaaa catggcactc aaaaaagaaa
     6721 ttatagataa atttaaagat acattgaaag aaatttcatt aaattaattt taaattatgt
     6781 taaaatatta ctaatgtaat aatgtgctga agggagattg tcatattgag agtaatttca
     6841 ggaaaagcga gaggattaaa actaaatact ccaaaaaatg aagatgtaag gccaactaca
     6901 gatagagtaa aagagtctct atttaatatg ataaattcat atataatgga aagtgaagtt
     6961 ctggatttat ttgctgggac tggttcttta ggaatagagt gcttatctag gggtgccaag
     7021 tcatgtactt ttgtagatat cagtaaggaa agcatagata ttgttaagtc caatgtaaaa
     7081 aaggcaagag ttgaaagtga aagtattatt ttaaaccttg attttaagac tgcaatagat
     7141 aaacttaaat tacaaaatag taaatttgac ataattttta tggaccctcc gtattataaa
     7201 aatatgttta tagaagctat tgaaaaaatt gataattcta atctattgaa cgaagatgga
     7261 ataatagttg tagaacatga tacgaatgat ttatttccag ataagatatg taaattggag
     7321 aaaactagag ataagaaata tggaaatact acattaactt tttataagat ggaggcataa
     7381 tatggaaaat aaacctagaa aagctatatt tgcaggaagt tttgacccaa ttactaatgg
     7441 acatttagat attatttgca gggcttcgaa gttatttgac gaattacaaa taggagtatt
     7501 aaataatcct aataaaaagg gtctatttag ctttgatgaa agagtaaaac ttatagaaaa
     7561 aagtacaagc catttaaata atataaaagt tgtgactttt gatggtttat taataaatta
     7621 ctgtcaggaa aatgggattg gagcattagt tagaggtgtg agaagtggtg ctgatgttga
     7681 ttatgaactt cagatggcac atatgaatag agagcttaat ccagatatag agactataat
     7741 tttaccaagt tgtacaaaat attcatttat aagttcatct ttaataaaag aggttttgct
     7801 ctttgatgct gatataaaaa atttggttcc aaagattgtt ttggaagaat taaagaaaaa
     7861 aactagtggg ggtaattgat atatgaagat agatttagaa atgattgaac tatttgagca
     7921 gttagaggag atatttagaa gtgcttctac aatccctttc tcacataaat gcacagtgga
     7981 taaagatgag gttttagcaa taattaatga tataagaact ttaataccag aagaagtaac
     8041 acaggcagta tggattaata aagaaagaaa taaaataata agtcaagcta agcaagatgc
     8101 tacaaatata gttgaacaag cgaaaaaaga agctgagaga atacaacaag aatatcaaga
     8161 taatatagag gaattaaaga aaaattctga agatgtagta aaggcatatg tagaatccag
     8221 cgaacctgtt gttcaagcag accaaagagc aaaagatata gttgatagag ctgagagaat
     8281 agccaatgag ataagaattg ggtcaattga atatgcagag gatgtgcttt ctagtgttga
     8341 gtataattta agagaaatat tagaagaagt aaaaagagat aaagctgaac ttagacctaa
     8401 aaaataaatt ttaaataaat aaaaatggag ctatcacaca aaaaattcca attgatgtgt
     8461 gatagctctc ttttaatata gcaatttttt gttatataat tgtgcttttt agattactta
     8521 tatcttattt tgatattata atgtagttta atcttttatt tgttgaaatc aacttattta
     8581 tttgtataaa taatataggt tattttcaaa tttttttaat aattttacca ctgtcttctt
     8641 ttagattagg gttagactgt gcaaaattat aaaataatac taaatcatgt gcaattattt
     8701 ggtatggcac tacatgtaga aaatatagat aataaaatta aggaggtagt atgaaggaaa
     8761 gggctaaaaa aatattagat tttattatta aaaataacaa tgtaacatca caagaactac
     8821 aagaaaggtt taatgtttca aagagaacta tttattacga tattctagct ataaatgaac
     8881 aactaggaaa aagtggtaat ataaaaaatg taaaacacaa atttatattt gaaggtaatt
     8941 tatgtgatgc aaggaaaata atttcaactg aagaagataa atttttagat agtgattaca
     9001 gaaaaacttt tatattaaac aagatattac ttggcgaaaa aatatcaata gaaaaactaa
     9061 caaatgaaat gttactttca aaaaacacag tagtacaaac aataactgat gtaaaaaaat
     9121 accttcaaac aatggggtta agattagagt ataagggaaa gtacaaaatt ataggtgatg
     9181 aatacgttat tagagagctg tttctgataa ttgttcaaga aaatgtactg gaaattaatt
     9241 ctataagtaa ggaagtaagt agtttcgata caaaaggaca tataaaactt actgattatt
     9301 ctttattaaa tctaactaag tttgtggaat ttctaaataa aaggataaga gatgggaaaa
     9361 cattatactc atataaatat ctaaatgaag ctaaaaaaat tagttacttt agtaattgta
     9421 aagaactttt atgtgaagaa gccaatgaaa atgaacaagc ctatatatgc acttacataa
     9481 gttcacttcc tagcttaaat agtgaagtta aggaagatgt tgttgaagaa tatgtagata
     9541 aacttataga taagtttgaa gtaaatacag caataaaatt agaaagtaag catgagttta
     9601 agaaaaatat tttaaggcac ctccactctt cttacaacag aatacgattt aagtttccta
     9661 ttagaaatcc aatgttagat gagactaagt ataaacatga gtcactttat aaaattataa
     9721 aatcaattat agaaaatgaa gaagagtttc ctgtatttga ggggataaga gaagaagaga
     9781 taggatttat agcagcgtat tttggagggt atttaagagg gtctagggat aatggcctaa
     9841 gaagaaataa agtcttacta gtttgtccta atggtcttat ggtatcaaag agtttggaaa
     9901 tacaactata taaatacata ccaaccatag aaatagtagg tatagtgtcc attaagcaac
     9961 taaaagaagt aaatgtatac tacgattaca taattacaac tatagatata caaaatgtaa
    10021 acaatgtaat tgttgtaaat cctcttttaa catcatcaga tgttcaatta cttatgaaca
    10081 aactaataag tgtaaaagaa aacgagaagt atttcaatct tgaattaata atacaagcta
    10141 taagaaaaaa tggagttatc aataatgaag aagcactaaa agctgattta ttaaacataa
    10201 tacacaagat agatgaagga gagatgtatc aaccaatgtt aaaggaatta attaatgcag
    10261 aaagggtcaa tataataaaa agtgttagag attggaaaga agcaataaaa atagcctcaa
    10321 agccattgtt agaagataac tctatagaag agttatatat agaaaatatg ataaagtcag
    10381 ttgaaaaata tggaccatat attgttttag ctgatagatt tgctctacca catgcaagtt
    10441 ctaaagaagg agtaaacaag cttgctatgt ctttactaat agtagaagat gaagttgatt
    10501 tattaggaaa accagtaaat atatttatgg tattagctgc tgtagataac actactcaca
    10561 ttagagcatt agcatcacta tcagagatga tgtacgagga agaaaatgta aaactaataa
    10621 ttaatggtga taaaagttca atactagaac ttataaataa gcaaaattag gaggaatgaa
    10681 aaatgaagat attagcagta tgtggttttg gtgtaggttc atcaatggta ttaaaaatga
    10741 caatacaaaa ggctctaaag cagttaggta tagaatgtga agtagaaaat acagatataa
    10801 attcagctag aggtgctgat tgtgactgta tattcacaag tcaagaattg ttaaatgaat
    10861 taaaaggaac ttgtaatgta gatgtatatc cagttaaaaa gtacatggat ttagcagaag
    10921 tcacagaagt tgttcaaact tttttaagtg ataaaaatga taagaaatag tataagtttt
    10981 aaaaagtaaa tttaagttaa tcagaataaa ataaaaactt actaaattca aaaatagtaa
    11041 gaaagagagg agggatttag tggtagaaaa cttaaacagt tgacctatca ctaaaaaata
    11101 taatgcttga ttttatttca aataatattt tacgtaatcc atctatgtta attggattaa
    11161 tagcagcatt tggtcttgta ctacagaaaa agaaagcatc tgatgttata aaggggtcac
    11221 tacttgcagc tttcggaatt ataattatgg agactggaac aggtatgtta gttagttcaa
    11281 tagcaccaat taatggagct ttccaaagta taagtggtgg tgcagtaaca aaaggtttaa
    11341 gtgatgttac ttttactgca agttatggtg gaattatagg tttagcaatg tttgcgggtt
    11401 tagctattca cctaatgata gcaagattta ctcctattaa gacaatcttc ttaacaggtc
    11461 acatgcttta ttggttccca ttcatatttg tagcttcagg agttgaagct ggattaaatg
    11521 gtggagtatt aatagctttt gcagcagtat tatcagcact ctattggtca ataatgcctt
    11581 ggttattaag aaaatatgtg tttgatgtta taggagatga ttcttttaca ttaggtcatc
    11641 catctggttg tttagcactt atatcaggat ttgttgctaa attagtagga aataaagaaa
    11701 aatctacaga agatataaac atacctcaaa gtttatcatt ttttagagaa atatctataa
    11761 gtggtggaat ggcagtgttt ttaatgttta tagttgttgg gatagtaata ccaggtgcgt
    11821 ttaaagatag taatgagttt attggaacgt atgcaataaa gcaaggtctt tcttttggag
    11881 caggtcttat aataatgctt catggggtaa gaatgctaat aaatcaaata atgccagcat
    11941 ttcaaggtat atctgaaaaa gttgtgccaa atgcactacc tgcttttgac tgtccaatat
    12001 tatttaatta taaaccaaat gctgtattaa taggattctt agttgctatg ctaacatcta
    12061 cagctatgat tttaatatgt aactacttta atgtatttaa tttgctactt ataccacttg
    12121 ttataacttc attctttgag tgtggagcag cagcagtaat aggagaatca caaggtggta
    12181 tacgtggagc tataataggt acagctatag ctgcaatatt tatggtagtt ttagtaggaa
    12241 tatctgctgt aatttactca ggaactattc aaaattggat actaatattt ggaggtaacg
    12301 acctttcatt atttggtata attagtaagt ttataggttc actatttgga ggaatattat
    12361 agatatgaaa tttaaataca tagatgagat aagaagatta attgatataa tagaaaacga
    12421 agaaaaagtc aatatggaaa aagcagtaca aatccttgtt gaagcaatag aaagtaaaaa
    12481 tgctatattt tcatttggag cttcacatgc aggaatatta acagaagaat tattctatag
    12541 agcaggtggc tgtgttctta taaatccagt atttgcaaaa tctctaatgt tagatacagc
    12601 gcctataact cacacaagta agatggaaag attagaagga tatggtacag ctctagctga
    12661 taaaactcca attaaaaaag gagatgtagt tatagttcat tcagtatctg gtagaaatcc
    12721 agtaagtata gagtttgcta tgaaagctaa ggaaaataag gcaatagtaa tatgtataac
    12781 taatttaagt tactcaaaaa gtgtatcttc aagacattct tcaggaaaaa aactttatga
    12841 agtatgcgat ttagtgatag ataatcatgg tcaaaaggga gatgcctgtg tagagataga
    12901 tggattaaac caaaaagtta gtccaacttc tactgtaata gcaactactg tgatgaactc
    12961 tattatagct caagctactc aggaattagt taataaaggg ctaaagaatc ctccgatttt
    13021 ctatagtgcc aatattgatg gtggagatga gttaaataag aagatttttg atgaatataa
    13081 aagtgtaatt cattatgaat attaaggtta aaaaaatatg ttaatgaact ttgaagttta
    13141 tcaacaaaga atattaataa tatataataa atgctatcat ttaaaagctg taggtaaaaa
    13201 tctacagctt tttattattg tagattaaaa agatgcgtaa ttaaatttta aattggagaa
    13261 aataaattgg aaggtatcaa ttataaatat tagaaacgtt gtcatagaaa aaaactatat
    13321 taaaaaaaag tacatagaaa aaactgatta gacaataaat acatgctttg ataatatttt
    13381 aaatggagtg aaaatgtgta gaattataat gaaaaaagaa aataaaaatg aaaaaagaaa
    13441 ataaaatgga gggaataaaa gtgggtatgt taaaaaaatt atgttcacta gctatggtag
    13501 caattatgac tacaacactt ataacagggt gttcaagtgg aaatggaaaa gattcaaaaa
    13561 aggatggcga aaaagaaaaa ctagttcttt ggatgccacc agtagaaaat aatatgaagg
    13621 aagtttggga ccctatttta gataaatttg aggaaaaaaa taactgtgaa gtagatttac
    13681 agataattcc ttgggataat tattctgaaa aatttgcaac agctataagt gctggcgaag
    13741 gtccagatgt aggatatatg tatgcagaaa tgtatccaca gtttatagaa agtggagcag
    13801 tagaagattt gactaactat gcaactaaag aggataaaga gcaatctatt tatataaaaa
    13861 catctgaaat gatgggtgga atgtatggta tgccattcca agcagcaaat ccaggggtat
    13921 tatactataa caaggatatt ttagagaaat taggagagaa acctcctaag acatgggaag
    13981 attttaaaag aatttgtaaa aaagcaacac aagatactga tggtgatgga aagatagacc
    14041 aatggggatt agctcaggga tggggagcta aaacttttgg taacttaaac tggaactggt
    14101 atccttattt atggcaagct ggtggagata tttttaatga tgatttaaaa tcggttaaat
    14161 ttaatgataa gagtggttta gaagcagcta atttcttaaa agaattacaa gcatatgtgc
    14221 ctgaagattc tttatctaaa gatagtaatg aaatgataga aagcgtattt ggaccaggga
    14281 aagcagcatt tacaataatg ttatcttctg ctgcaacaag tgtatttgat gaatcattcc
    14341 cggatttaaa ttgggggttt gtaactggac tagaagataa gaaagcagca acttttggag
    14401 cagtagacca tttatcttta atgtcatctg ctaaaaataa agaacttgca tacaagttaa
    14461 tacaacatat gttaagtgtt gaatcaatga cagaatttca taaagcaata ccaagagcac
    14521 caataacaaa aggtgaacca tatcaaggtg atgaaagatt caaagaaatg gtggaaaatg
    14581 acaaaaatgt atatagacca ttagtagtag gacctcatgg agttgaaata tatgaatatt
    14641 tatggaaaga acttcaaact atgatttcag gagataaaac tccaaaacaa gcattagatg
    14701 atgctgcaaa atattcaaat gatttattag cacaataaaa gtttaaagct gtaatatgtt
    14761 gtaaaggact ttacagatta aaattagcat tgtatttata aatacaatgc taatatttat
    14821 attggagagg ataaaatgga aaacttagtt agagagtttg aagaaaattc aaaaagaaaa
    14881 aaaacaaaat taaatattaa accatatatg tatatattgc cattgggaat aattttagtg
    14941 gcgttttatg tgataccaat aataatgtca atatacttta gttttacaaa gtataatatt
    15001 attagtccag ccacttttat aggtttagaa aattataaaa aattatttac agatgaaaca
    15061 ttaaaagtat ctattataaa tactattaaa tttactgttg ttgtagtacc ttgccaaact
    15121 atattatcat tgattttggc agtttggatt acaggaaaag gaaatagtaa aatagcttca
    15181 tttgctaaag gtgctatatt tataccagta ttatcatcta tggttttgat aggtatggta
    15241 tggagggctt tattgaatgg agaaggttct attatatatc aggtattagg tgcctttgga
    15301 atagaatcat ctaaactttt aggagatagt aagacagcat tacctacact tatgtttata
    15361 tctatgtgga aaaatatagg gtatttcatg gtaatttata tttcagctat aatgaattta
    15421 ccaaaacact gctatgaagt agcgaaggtt gatggggcta caaaacttca agaatttata
    15481 aaaataactg tacctttatt gaaaccaact acaataatgg ttgtattttt agggtcgata
    15541 tggtcattac aagtatttga cttaatttat actgtgacag gtggaggtcc aggtatatct
    15601 actatgagta tagttatgca tgcatttaac ttaaacttta aaaactttaa ttctggatat
    15661 gcaatgactg tagcaaacgt attatttttg ttaatagcag tagtttcgat tttacaaaat
    15721 aagcttgtaa aacgagacaa ttcagatttt tagaaaggta ggtataatat gaaaacagca
    15781 agaagattat taagtgatgg tttactttta tttatagcgt gtgcaagttt ggtacctttt
    15841 atatacatgt tgataatatc attgaagata acatataact catatagttt ggatatatct
    15901 ttttcaacag taactcttca aaactatata gatatcttta ctaaaaaagg atttgcacaa
    15961 tattttttta atacagcaat agtatctttt tcaggtgtat tattaaattt agtatttagt
    16021 acattagcag gatattcttt tgcgaagatg gattttaaag gtagtgataa gttattttta
    16081 tttatgataa tgactttaat tataccttcc caagttacaa tgataccact atatataata
    16141 atgaaacact taggatggat aaatagttat cttgcactta ttatgccaat accaactgca
    16201 tttggcgtat ttataatgag acaagctatt ttaggagtac caaaagagtt attagaatca
    16261 gcaaagatag atggttgctc ggattttaga attttaatac aaatagtatt gcctttaatc
    16321 aaaccagcat taattacact agcaatattt acttttatgg gagcatggaa cgagtttatg
    16381 tggcctctta tagctacaac taaagatgca atgagaacat taacagttgg attatctact
    16441 cttaagacat tccagataac taattatgga cagatgatgg caggggctac aataacattt
    16501 ttacctccat ttattttcta tttgatttta caaagtaaat ttgttgaagg tgtgtctttg
    16561 tctggtataa aaggttaaat agtctaattt aataacataa aagaaactta tataaatatg
    16621 gaggaattta aactatggaa atttcatatg tactgaaaga ttgcacattc aaacacaata
    16681 aatactcagt tatagaagct gaaacttgga aagagaagga tttggatatt gtagtagcta
    16741 aaggtgaaaa atttgcattt caaattatgc taaaagcaac agaagaattt aattgtacaa
    16801 ttgataaaag cagtactata tcatggaaag gactggaaaa tagagtaaga ttagcattaa
    16861 atgtgcctaa tagtttagaa aataatttta gtataaatat attaggatat gttcaagatg
    16921 atacaaaggc atttgtgaat gatgctattt taagggataa agatgtgtta gtagagcaat
    16981 atttgcctca aacattttgg attgaaggtc aagtaccaga agattttgaa gaaaataacc
    17041 ttaatatagg tattgatata tttaaaagct ttggatatga agatgaagaa aaagtttgta
    17101 caattgatgt tcctgtaaaa gtaagaaatg tcgtgctaaa gcctttagat aaaagcaagt
    17161 tctttttaga tttatggcaa catccaagtt gtttagctag aatgtataaa gttgagttat
    17221 ggtctgatat acattttgaa atagtcgata actatttaaa agaattagca tctttaggag
    17281 aaaaagttgc aacagttata gtttcagatt atccatgggc agggcaaagt tgttataaag
    17341 tttataaaaa tccatcaaac ctatatgaat ataacatggt aagtgtatca aaaggattgg
    17401 atggaaagat taaatgtaac tttgagagta tggataggta tataagcata gctgataagt
    17461 ataaaatgtc taaggagata gatttatttg gtctattagg taattggtgt gctggtgagt
    17521 ttggaaatcc tgtagaaggt tataaggacc ctataagagt aagatatttt gatgaaaatg
    17581 ataaagtatt taaatttata aacaatacaa atgatttaaa agaatatata ggattagtat
    17641 taaatcattt aatagagtgt ggattatggg atagagtaag gattatagct gatgaaccaa
    17701 ataatccaga agtagtcaaa gagtgtatag aatttataaa ttctactgta ggtactcatc
    17761 aagttaaata taaatcagca actcacgacc aaaacttttt agatagagct aaagatgaaa
    17821 ttgatgatat gtcaataaat ttaaaactta ctatacaaaa ttataaagat atagaaaatc
    17881 ttaagaaaaa aatcaatgac aagggtggga tattgacttg gtttgtatgc tgttttccag
    17941 agaaaccaaa tagtttttta agttctcctt ttgtagaaaa tagaataata ggatggtata
    18001 cttattactt tggtcttgat ggatttttaa gatgggacta taatttatgg acagaagacc
    18061 cttggaaaga ttcaagttac aaattcccta tttggaaagc tggagatatg ttctttgttt
    18121 atcctggaaa agatttaaag ccagttagaa gtgtcagaat ggagaattta agatttggaa
    18181 tacaggactt tgagttattt acaatgttag aaaaagaaaa gggcagagag tatatagaag
    18241 ttgagcttat gcaagaatta ttaaataaaa aagaaaatgc tgaaattaaa ggttttggtg
    18301 acatagaatt aggatattca cttgataatt ggggatatga taaagttaaa aaatatgtat
    18361 tagagttgtt agacagagta taaaaaaata taagagtgtc tcaaaatgaa ctttttagtt
    18421 catgaggcac tctttttgta tgaaatcaat atagtttcat tgtttcaaca atgaaaaata
    18481 ggattactta tattctaaga tagcccttta gttttttagt tgtattcaat gttttaaaac
    18541 taactataaa cgctataaag tttataaatg acaataatta tatgtaacat gatgtacttt
    18601 aatttgctat aatagggatt gaataatatt atatggaaga taacattgtt tggggggatt
    18661 aatataaaaa gaataggtat tattataatg ttttttttaa gtatagcttt tattggattt
    18721 tatcttgcaa taagaggtaa cagtgataca gttaagatgc aacgagggat aaatataggt
    18781 aatgcactag aatcacctaa ggattttccg tgggatgtaa aaatgtcaaa taagtttttt
    18841 gatgatataa aagatgcagg ttttgacaca gtacgtatac cagtaaggtt ttctgattat
    18901 acgtcagatt ctgataattt taaaattgat gaagaatttt ttaaaaagat tgataaatat
    18961 gtagattatg cattggataa agacttaata gttgtgttgg atttacatca ttttgaagaa
    19021 attatgaaag aaccaagagt tcataaagaa gagtttttaa aaatatggca acagatagct
    19081 aataggtatc aaaaatatga taaaaaattg gtatttgaat tattaaatga acctaaagaa
    19141 aatttatctt cacaattact aaacgaatac attgaagaag caataaagat aattagaaaa
    19201 actaacccta aaagaactat tatagtggga ccatataatt tctatcaaat tgattatttg
    19261 aatgaattaa atgttccaaa agattctaat atagtagttt cttttcacta ttacgaacca
    19321 aatgattttg catttcaagg aaacatttat cataatggat ttgagcactt gagcaatatt
    19381 acttgggaag gaacaaatga acaaatggat tatttaaaaa aaaggtttga tattgtagaa
    19441 aattgggcaa ataaaaataa ggtaaaaata ttcttgggtg aatttggagt aacaaaagaa
    19501 gcaccagaag cttcaagaag agcttggata aaagctgtaa gagaagaagc tgaaaaacga
    19561 aacttttcat gggcttattg ggagcttgct tctggatttg gtatatacaa tcaaattgaa
    19621 gggacttggg atagagacat attaagtgca ttaatagaaa agaggtgatt ttatgaagaa
    19681 aaattcaaaa aagatactta taactttaac tattattact aatatcgtat atatattgtg
    19741 gagaattttt tacacagtac ctaaagaaga agggatgttt gccttaatct gtgctataat
    19801 acttcttttt gtagagataa tgggtatgat ggaaatgttt gtacattatt atggtatgtc
    19861 aaatattgag tatcctgaaa aaccagtcat aagtgaagaa ctttatccac atgtagatgt
    19921 attcattgca acatataatg agtctgttga tttggtgaga aaaactgtaa atggatgtat
    19981 tcatatgcag tatccagata agaaaaaggt acatatatac atatgtgatg atggaaatcg
    20041 tgaagaaatg cgtattctag cagaaaaaat gggtgtaaat tacataacta gaactgaaag
    20101 agaaggagca aaagctggga atttaaataa tgcaatgcaa catacaaact ctcctttgat
    20161 tgctacgttt gatgcagata tgataccaat gcatgacttt ttaattgcta cagtaccata
    20221 ctttttgaaa aatgagcaag ctaaaaaaga tggagaaaaa gaggagtatg aaaaggttgg
    20281 ttttgtgcaa actccacaga gtttttataa tccagacttg ttccaattta atctgcactc
    20341 tgagggaagg attccaaatg agcaagatta tttttacaga gatattcaat tggctagaaa
    20401 taggacaaat tctgtaattt atggtggtag taatacagtt atttcaaggg aagcacttga
    20461 agaagttgat ggattttata cttattctat tacagaagat tttgcaacag gtatcttaat
    20521 tcaaagtaag gggtatagat gttatgctat tccagaagta catgcatcag gtttatctcc
    20581 tacagattta aaaagtctta ttaagcaacg tgaaagatgg gcacgtggat gtatacaaac
    20641 tggtagacgt ctaaatattt tgtttaggag aggtttaggt ttttggcaga aaataagtta
    20701 tatttcttca attacttatt ggtatgcaag tataaaacgc tttgtatata taatggcacc
    20761 tattttattt tctgtgttta atgtgattgt agtaaaatgt acattacttc aagtattggt
    20821 attttggttg ccaatgtata ttttaagtag tttatctctt aaaatatttt ctcaaaatat
    20881 aagaaatact agatggacca atatatatga aactataatg tttcagtctt taatgccagc
    20941 agtaatactt gaaacgtttg caatatctaa aaataaattt tcagtaacaa ataagaataa
    21001 attagaagaa aatagaatgt ataagttttt acagggaata ccttatttta tttacatggt
    21061 cttatctatc ataggaattt taaaaatgtt tgtagcaata tttaaaatga gttcaatgac
    21121 atattcagtt gtattatttt ggttgattgg aaaccttttt aaccttgtta tggctacatt
    21181 atttatatca gggagacaac agttaagaaa gtctgagcgt tatattgctg aaattgattt
    21241 taagttaaag caaaactctt atgttctttc ttcaaaaacg attgatatat cagaaaatgg
    21301 gtttgcattt ttgctagaaa atccagaata tatttcccca gaagaagagt tcgaggtaga
    21361 gtttagagaa aaatcaggaa atgaaatgta tatagctaat atgaaagcaa agattgttaa
    21421 tgttgtggaa gtaaattcaa agtggaaata tgcagcttat attacacata ttgaagatag
    21481 tgaaatagat aattggatgt gtattgttca cgatagaata cctactttac cgatgacaat
    21541 atctaatcaa ctaggtttct ttgatgattt gcaaattaat gtaaaaaaac gtattgaaaa
    21601 gattagaaca ttatcacgaa gaagtccaag aatcaatatg aattttcaga tggatataaa
    21661 aaatacaggt aaattaagaa tagtaaattt taattatcaa tatgtattat taaattttga
    21721 gaataaaaat atttatccta aagaaatagc attggaaatt aatgaaaata ttgtgttaga
    21781 atgtgattta tgtgagggta aaatcgatga aagaggtctc ttatataaag taaataacat
    21841 tgacagcata atgcaaaatt tctttttaag agatgaaatg atggattgga tattacaaaa
    21901 taagacaatt cctgtttcaa atcctagtga aaagaaggaa aaaagtatag atgagtttga
    21961 acccatggaa tatatttagg agagatgaaa tgaagacttt aaaagtgttt ttttgttctg
    22021 caaaatatct tattttggtg tttatttttt ttataaatac tacttttata agttcagcga
    22081 caagtgataa aattttagat ttatccttta aaaagataga gactgattta agttctagga
    22141 ttacatatga agatacaggt gtaaaaatag aaacagatag ttctaaaagt gataaggaac
    22201 gttatttata tatttaccaa aatataaaag aaaattggag tatgtataat aatttttata
    22261 ttgaaattca aaataaaaat aaaagttccc agaaaataaa tttaagtata caaagtaaga
    22321 atatgcttga atttaggtta aaagaaggta gtgaaatatt tttggaagat aaaaatataa
    22381 tatattcaga taaaataaaa gaaggatgca tagaagtacc aggtgaattt gaaggcaaaa
    22441 tttatgtgaa ctttaatagc cttataaatg agaaaagtaa tgttgtgctg gactcaagta
    22501 tgctttcaaa cattgttagt tggggaatta catttattcc atctgatgaa gaacataata
    22561 ttgttattat aaaaaagata tctttattgt ctgaagagaa attaagtttt ttacataata
    22621 ttaaaataac tggcaatgaa gaagtacaaa taccagtatt gggtcaaagt ataagtcagt
    22681 atgaggtatt aggtcttaaa cgtgattcta aaattaagta ttctcttatg ggaaaacaag
    22741 ataatgtgag catatctcaa aatggtaaat taacattaag taataaagcc gaacctggac
    22801 aaattatttt acaagttaat gttgatgata agtttaaaat tggaaggaaa atagcattaa
    22861 ctgaatcttg gagtattaat aaaaaagata aggatggtgt tccatataca ttggtatctc
    22921 ctgagcaatc accaacagta caatatatga aaaaaataaa ctttatgaat aacatcataa
    22981 cgtttgttcg aattttattt gtatctctag taataatatg ctttggaata tatttatatt
    23041 ggaaaaaatg ttcaaaaact aagtaaagag aggaggttag attgtgttat tttcaagttt
    23101 aattttttta ttttattttt tacctataac tttggtttta tattatgtat ttaggtttaa
    23161 ccgaacgata cagaacatga tattgcttgc tgttagttta tttttttatg cttggggaga
    23221 gccaaaattt gttgttatca tgatagtttc cataataatg aactatatat ttggtttact
    23281 agtagataga tatagggaaa gtaaaataaa agtaaaagtt tttttagttt tgatgtgtgt
    23341 atataatata ggtgttttat ttatatttaa atatctagct tttgcgctaa gaaatattag
    23401 tgaacttatt aatactgaac taactatacc tagtattgtt ttacctatag gaatttcatt
    23461 ttttacattt cagggaatgt cttatgttat agatgtatat cgtagacatg gagaagtcca
    23521 aaagaatcct ttttatgtgg gactgtatat agcatttttc ccacaattaa tagcaggtcc
    23581 tatagttaga tatgagagtg tggcagaaca aattctcaat agaaaagaaa cttggaataa
    23641 attttctatt gggacttgca ggtttataac aggtctaggc aaaaaagtat taatttcaaa
    23701 taacatggct atagtagcag actatattta cactatgaat agtcaaggag aaatagctgc
    23761 atcacttgca tggcttggtt caatagccta tactatgcaa atattctttg atttttctgc
    23821 ttattcagat atggctatag gtttaggtct tatgtttggt tttaaatttg aagagaactt
    23881 taattatcca tatatatcaa aatcaattag tgagttttgg agaagatggc acatttcttt
    23941 gggaatgtgg tttaagagct atatatactt tccgcttgga ggttctagag tagcaaataa
    24001 agatattatg attagaaata tgtttatagt gtggttattt actggaatat ggcatggagc
    24061 agaatggaca tttgtaattt gggggatttt aaactttgtt ttcttgatta tagagcgatt
    24121 tataatgttt gaaaagattg aaaatcacaa ttttataaaa catatttatg cgttattagt
    24181 agttaacttt gggtgggtat tgtttaggtc acctaattta aaagaagcat ataattattt
    24241 caaagctatg cttggaggca atggagttat atggagtgat tatacatata tgtttttaaa
    24301 agaatactgg gttttcttta tatttgcatt tatttttagt gtacctattg caaagaaaat
    24361 caataagttt gttgttgaac aagctaaata tagtatgata tttaacgtat tttatccaat
    24421 ctcagctatt ttattgttct ttatttctgt gacatattta gtaactggaa gctataatcc
    24481 atttatttat tttaattttt agttgggagg aaaagtatga atagattttt taaagtaaga
    24541 ttaataactg caattatgtt cttaattatt ttattcgctt tctctatctt taatatttgt
    24601 tattctggaa aagatatgat acaggagata aaaaattcag ttgctgaaaa aagtagttta
    24661 aaagatacaa tatcatctat tgataacaca gtaaatgaca aagtaatata taaaaatacg
    24721 ttcattgaaa cttatggcta cttacaaaag ttaatgggta aaaatgagtt ttcaaaattt
    24781 agtgttgtaa aagatacttc tggaaagatg cattatacat attttgctaa agctccaaat
    24841 ccagttggtg tgatagcaga tagggtaaga aaattttctg aggaaatgga aaagcaaaac
    24901 actaaattag tttacttaat gactccagat aagtatataa aaggagtgac aaaatttcca
    24961 aaaggaatac cttattctta tagcaatgag acagcagata attttttaaa tcaactaaaa
    25021 aagtataatg tggactatat tgattttaga gaaaatattt taaagagtgg tattccaaaa
    25081 gatgatttat tttttaaaac agaccatcac tggaaagtag aaacttcttt ttgggcattt
    25141 ggagaattag ttgagcaact taataaaaaa tacaatatga atttagatga aaatcattac
    25201 tatagagata aagaaaatta taactcaatt gtatatccta agtcatttct tggttctatg
    25261 ggaagaaaga ctggtatctt atatggtggt atagatgatt ttacattaat ttatcctaaa
    25321 tttaagacta attatactta ttatacagat tctaaatctc aaaaatttga attaaaagga
    25381 aggtttgaag agagtttaat attatcatat ccttttaatg cagaccttga tttaatggat
    25441 ggtcaatcag ataagtattt tacatattta ttggggaata gaccattagt taaaataaaa
    25501 aatatagaaa atccagatgg attaaaagtg ctgtttgtta aagattctct aattgtacca
    25561 acagcagcat ttttttcctc tgtttgttca tctatagata tgatagaccc tagatattat
    25621 gatggagata tactagaata tgcaaaatcc catgactatg actttgtttt tatatctgta
    25681 tatccccaaa atcttacaaa agaatttttt ccgttttgta agtaaatttc taaagaaatt
    25741 ttcttataaa ataaaaaata aatggctaac tagttaaaag ttagccattt atcttaagtg
    25801 ttactctaag aaatgttttt tttaccaaat acttctttat ctatttttcc aagtgatttt
    25861 cctacagtaa ttgatgatac catagcacca ttaacattta tcatggtacg tcccatatct
    25921 agtatagggt ctattgcaag tataccacca actaatggaa aataagaacc aagaccaact
    25981 ccagagataa caactgaaac ggccattgta gcagttcctg gaagaccagc tattccaaac
    26041 gaactaattg ttataacaat taacaacata acataaaggc tcatatccat gtttactcca
    26101 gccatattgg caagtgttac agccattaaa gcaggatata tacctgcaca accattcata
    26161 cccatatttg cacctaaact tgctgtaaag cttgatacac cctcatcaac tccgtgattt
    26221 tgtactagac tttctatcgt tacaggcaat gttcctaaac tagaacgtga agtaaatgcc
    26281 agaagtaaag cttcagttga atttcttata taagtaatgg gatttagacc atttaaagtt
    26341 ataaaaatta aatgtatggt aaataatatt gccactgata tataaagagc taatataaac
    26401 tgtaaaacag atactattga gctaatacct tgtgaagtta ttgtatttgc aagcagagca
    26461 accacagcat aaggcataaa tttaataaca gtcatagaca cacttacaat tattttgtaa
    26521 gatgcttcta taaaatctat aaatggtttt attgtttcaa agtgtttttt acttaatctc
    26581 tttattgagg tagctataaa tgtagcaaat attattgtag ctacaatatt tccatttgcc
    26641 atagccatta caggattaga tggtagtaat cctcttaaag tatccacaag agagtttatt
    26701 tctttaagtt cttgattttc aactaaagta ggcatattag ctcctacacc taacttcatt
    26761 atatttgcaa cgataattcc aacaattgca gctatagcag tagtagcaag aagcatacct
    26821 aaagatttaa atgtcagagt tccaagattg ttatctttca tatttataat tacttttata
    26881 atggataaga aaactaaagg tactactaac atttttaata agtccataaa tccagaacca
    26941 aataggctat accacttgct tacttcttgt agccaagtga cttgagtggt atcattagga
    27001 aaaccagcaa aagcttgtat tatgattccc aatatgagtc ctagtactgt agcatatatt
    27061 gttctcaatg aaaattttat ttttttcttt tcaaattgtc taacaataaa gaa
//
LOCUS       DAGQWR010000047        27079 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.047, whole genome shotgun sequence.
ACCESSION   DAGQWR010000047 DAGQWR010000000
VERSION     DAGQWR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27079
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.047"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            200..1591
                     /locus_tag="MIW95_001705"
     CDS             200..1591
                     /locus_tag="MIW95_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBY3151034.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1844..3637
                     /locus_tag="MIW95_001706"
     CDS             1844..3637
                     /locus_tag="MIW95_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY3151035.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILDELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3718..5100)
                     /locus_tag="MIW95_001707"
     CDS             complement(3718..5100)
                     /locus_tag="MIW95_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBY3151036.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5358..5705
                     /locus_tag="MIW95_001708"
     CDS             5358..5705
                     /locus_tag="MIW95_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBY3151037.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5723..6757
                     /locus_tag="MIW95_001709"
     CDS             5723..6757
                     /locus_tag="MIW95_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151038.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVVWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6806..7855)
                     /locus_tag="MIW95_001710"
     CDS             complement(6806..7855)
                     /locus_tag="MIW95_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY3151039.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENRVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8130..9665
                     /locus_tag="MIW95_001711"
     CDS             8130..9665
                     /locus_tag="MIW95_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151040.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFNLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9851..10537)
                     /locus_tag="MIW95_001712"
     CDS             complement(9851..10537)
                     /locus_tag="MIW95_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBY3151041.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEGVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10662..12044
                     /locus_tag="MIW95_001713"
     CDS             10662..12044
                     /locus_tag="MIW95_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY3151042.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            12446..13714
                     /gene="eam"
                     /locus_tag="MIW95_001714"
     CDS             12446..13714
                     /gene="eam"
                     /locus_tag="MIW95_001714"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBY3151043.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(13807..14163)
                     /locus_tag="MIW95_001715"
     CDS             complement(13807..14163)
                     /locus_tag="MIW95_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151044.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(14314..15408)
                     /locus_tag="MIW95_001716"
     CDS             complement(14314..15408)
                     /locus_tag="MIW95_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBY3151045.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
     gene            15872..16693
                     /locus_tag="MIW95_001717"
     CDS             15872..16693
                     /locus_tag="MIW95_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBY3151046.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            16714..17910
                     /locus_tag="MIW95_001718"
     CDS             16714..17910
                     /locus_tag="MIW95_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBY3151047.1"
                     /translation="MFIEFFYLLRSRGLNISLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMLSR"
     gene            18076..21474
                     /locus_tag="MIW95_001719"
     CDS             18076..21474
                     /locus_tag="MIW95_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBY3151048.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVVTVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIISFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSSINQDLETEDIGYRSINKSFKDEENRYKFIDGYNIQI
                     HNDLENEIYISKNASRQSGILSGIDVVHTPEFEEELAGLGMSQNFFKISDSLGVLSIN
                     NTDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICSGLGNVNSEYAGIAEDSELIIIKLGKIDGFYNS
                     AMLFAASQYAYKKAFELRRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPHVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITVGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            21484..23157
                     /locus_tag="MIW95_001720"
     CDS             21484..23157
                     /locus_tag="MIW95_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBY3151049.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPVDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSQLNIAINRND
                     GSLSQDNIGTGTLVSGILAGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     ISGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            23431..23625
                     /locus_tag="MIW95_001721"
     CDS             23431..23625
                     /locus_tag="MIW95_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBY3151050.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    23794..24027
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24104..25501
                     /gene="asnS"
                     /locus_tag="MIW95_001722"
     CDS             24104..25501
                     /gene="asnS"
                     /locus_tag="MIW95_001722"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBY3151051.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(25674..26849)
                     /locus_tag="MIW95_001723"
     CDS             complement(25674..26849)
                     /locus_tag="MIW95_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY3151052.1"
                     /translation="MNLEKIKKFFGFPLDKKHKENVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFATPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCDETDAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIEKAITLNEFISNCDKALYTAKEQGRNCVAFY"
BASE COUNT        10445 a         3242 c         4582 g         8810 t
ORIGIN      
        1 atatatatta agattttcta ctgaagatac taaaaaaata atatttttca taattttaaa
       61 aataataata aaaaaatgaa aatacaggcg ataatctatt gagtaaacat agaaaagatg
      121 atattataag tatgtgctaa aatggttaaa gcaatattaa tattaaattc aactataaaa
      181 caaaaggggg cgttttttta tgtcaagtac agaaagcgta tctaaaaaaa gtggtcatcc
      241 gaagagtttt tggcttattt gcatgacaat tttgtgggaa agatttagtt attatggttt
      301 aacagcttta ttggtattat tttttacagc aagtgtagct caaggtggtt taggtctttc
      361 tactcaagaa gcaacatcca tgtttggctt atttacaggt cttatatatc ttacaccatt
      421 agtcggtggc tggttagcag ataaagtttt aggtcaacaa aaatgtattt ctataggtgc
      481 acttttaata gcaataggag atttcgtact atttgcttca tctacatcta ttgccatggt
      541 ttgggtaggt ctatcaataa ttattttagg aaatggtttt tttaaatcaa gttgttcaaa
      601 tatagttggg gatatatatc ctaaaactga tgtgtcaaga aaagatgcag catacagttt
      661 attctatatg gcagtaaatg taggagcatt tatagctcca ataatatgtg gattgctttc
      721 tgataatgta tttgcagtaa gaggagcaag tggagatata gtatcttatg gatataagat
      781 ggcattttta atttgtggta taggcatgat gttaggtatg attatattta cacttcttgc
      841 accaaaatta ttaaatgaag tcggaaaata tcctgtgact aaaggtaata aaggtgaaaa
      901 aattgagtat ggaccactta ctaagttgga gaaaaatagg ataatagcaa tggttatttt
      961 atttttattt gtggtcttat tctggacagc atactatcaa acacaaagtt cttttatgat
     1021 ttatgtaagg gataatgtaa atagaactct atttggattt gaaatgccag tagcatggct
     1081 tacatcacta aatgctatat tatgtgtggc tttatgccca gtattagcta ccctatgggt
     1141 taaattatct cataccaaaa gaggtgattt ctctattcca gttaaaatgg gattaggaat
     1201 gataataatg ggtgttgggt ttatagttat ggtatttgct acattagcta atggtccaga
     1261 tggaatggta aaggctagta tattgtttat agtattgact tatatattta caacagtagg
     1321 tgaattattc ttatctccta taggacttgc aatgttcaat aaattagctc cagctaagta
     1381 tgctacatta tctatgggag cctggtatct aacaagtttt tttgcaagtt tattatcagg
     1441 aaagattgct ggttttacag ccacacttgg ttttttacag atatttggag gaatagcaac
     1501 agttttaata atatttggtt taatattatt gggaataaga aatgtattaa caagattaat
     1561 ggctatggat ttactaacaa aagaaaaata attcatgacg aattattgta aatagatgaa
     1621 ttacttcaaa taaaggaagt tacatttaaa tataaaatta aatgtaattt cctctttgat
     1681 tatgaaaaaa agctttacac aaatagatat tataattaaa attataattg tgataattag
     1741 agtgtataag atttatataa attatacaaa aaatatttat gtaataaact ttacatactt
     1801 atatatttag ttattcaaac tattatttcg aggaggtctt gttgtgaata taaaagatag
     1861 attaagtgga ttaagaaaat ttatggaaga aaaaaatata gatgcctata tgattccatc
     1921 ttctgataat caccaaagtg aatatgtagg agattacttc aaatcaagag aatttatatc
     1981 agggtttaat ggttcagcag ggacggttat tgtgactaaa gacgaagctg ggttatggac
     2041 cgatggaaga tattttatac aggcagaatc tcaattagaa ggaagtacaa taaaattatt
     2101 caaaatggga caagaaggtt gcccaactac agatgaatat ttatataaaa atattcctga
     2161 aggaggaact ttaggatttg atggtagagt tatatctgct agagaaggag ctacattagc
     2221 tgaaaaatta tctaaaaaag gtataaagat agaatatcag tatgatttaa tagatggtat
     2281 atggccagat agacctgctt tatcagatag taaagcattt ttattagatg ttaaatattg
     2341 tggagaaagt tttagctcaa aattagctag attaagagaa aaaatgagtg aaaaaggtac
     2401 ttctactcat gtaataacta ctcttgatga tatagcatgg ttattcaata taagaggtgg
     2461 agatgttaaa tataatccag ttgtactttc ttatgcagta ataactttaa aagaagttta
     2521 tttatttgtt gatgaaagta agttaaatga agaaatatta gatgaactag ctaaggaaaa
     2581 tgttcaaata aagccatata atgatgtgta tgaatttgta aaaaatatag acaaaactga
     2641 aaaagtttta ttagatggaa ctaagcttag ttatacaatt tacaataata taccttgtga
     2701 agttgagaaa gttgatgagt tcaatccagt aatgtttttt aaagctcaaa aaaatgaggt
     2761 agaattagaa aatataagaa attcacatgt aaaagatggt gtggctttca ctaaatttat
     2821 gtactggtta aagaaaaatg taggtaagat ggaaatcact gaaataagtg ctactcaaaa
     2881 attagaggac ttaagaagag agcaagaagg tttctttgag ccaagtttca atacaattgc
     2941 tgcatataaa gagcatgctg caatgatgca ctatagtgca actccagagt caaattataa
     3001 attagaggca gaaggattat ttttagtaga ttcaggtgga caatattatg atggaactac
     3061 tgatataact aggactacag ttttaggacc tataagtgat gaattaaaac tacactttac
     3121 ttctgtagca agaggaatga taaatctttc taaggctaag ttcttgcatg gttgtagagg
     3181 atataactta gacatactat ctagaagttg catgtggaat atgggaattg attatcaatg
     3241 tggaactgga catggtatag gatttgtgct aaatgttcac gaagctccaa atggatttag
     3301 atggagagta gtacctgaaa gatttgatag tgcagttctt gaagaaggaa tggtaactac
     3361 taatgaacca ggtatatata tagaaggttc tcatggtata agaacagaga atgaaattgt
     3421 agttagaaaa gctgaaaaga atttctatgg tcaatttatg gaatttgaag ttgttacgtt
     3481 agctcctatt gacttagatg gtatagtacc agaattaatg aataaggatg aaaaagatta
     3541 tttaaactgg tatcataagt tagtttatga taaaatatca cctttcttaa ctgatgaaga
     3601 aagagaatgg ttaaaagttt acactagagc tatatagttt gtactagata caataaaaat
     3661 cgaggtatta taatgcctcg attttttatt gtatatttta ttaactttaa aaaattttta
     3721 atttgataat tctactaatt ttttattcat tataagtagt ataattccaa atatcaataa
     3781 aaatgatggt atcactatga agacttgtaa cataccaagt tggtctataa tagcttgcgt
     3841 atagccagat aatttatttg ctgcaaattt gcctaaaaac caaacaccca ttaggaatga
     3901 aatatacttt tcaggcgaaa gtttatttac cataccatac ccgactggag aaaagcaaat
     3961 ttctcctata ctttggaatg ttaaaaatcc aactacccaa aatacacttg ctttgtgaat
     4021 tgaattaggg tctgttcctg tttgtataac tgctactatc ataaacaaaa atgctatagc
     4081 taatagtata aaacctaagc tcattttttt tggcacactt aaatcgcctt ttttagtttg
     4141 agataatttt acccatacta aagcagagat aggtccaaga ataacacaaa ggagaccatt
     4201 gtatgaatct atccatgaag taggaatttg aaaattgcca acagtaagat ttatgtgttc
     4261 ctttgtatag agtgcaatag acatagaggc ttggttatat gctatccaaa aaaatactgt
     4321 gaaaaaagct aacaccaata ttactattat tctttttttc tcaatcaaag ttaaaggtct
     4381 tgaagcgagc attttcttat attctagtga ttttcttata gactctctcc cgttactagg
     4441 gtcattattc aaaggtttat aaaactttct gtttccaaat ataaagaaga ataaacctat
     4501 tagaaccgta attgatgaaa ataaaaatac atatctatat ccatacatac ttatagataa
     4561 tccgcttttc atagcaaacc attcattagc aactaaaccg catattaaag ggccaaataa
     4621 agtacctaga ttagtagcca tatacgtaat agagaaagct ccatcttttc ttgggtcatc
     4681 attggtatat aagtttccaa ccatagtatg taagtttcct ttataaaaac ctattcctaa
     4741 tccagataaa aatatcatga tgtttatatc catcttacta tttgctatgt atcctagaaa
     4801 ataaccaatt gaaaccatta gcattccaag tataatacag tttttgggac ctaaaaatct
     4861 atcactgacc catccaccaa taactggaga gaaataagtc catgctaaaa aagatgcaag
     4921 cattgatgcg gattctgctg gtgttaaccc taaaccacct ttggagactt gttcagctaa
     4981 aaacaataat aataccaatt tcataccata gtatgcaagt cctacaaagg taaatattga
     5041 acagcagaaa tataaatctc taggttgttt aagtttttct tgcgttttta aaactgacat
     5101 atgtcgagaa cctccagata aatagtttaa caaaatttta ccatactata tgagtatatg
     5161 tttttttcat atagacgtca acttgtaaga taatttaatt aagtcgaaat atatgtaaaa
     5221 atagtatcat ttttgtgtat tcagtaatat ttttttatga taatatatgt tattattata
     5281 ttaaaataca aacatattta tggaaaggat atgccaattt agtagttaga ttgattgttt
     5341 ggcaggtggg atgagctatg ataagaaaga aatctgaatt acaagtagaa gtaaaagaaa
     5401 atttaaatgg tggtatagga aaggttaagc ttgaaaacat aatacaaaat gaagagctta
     5461 aaggaaaagg aagactattt aaaagagtag ctttaccagt aggaagttca attggtgtgc
     5521 atgaccatac aacagatttt gaagtatatt atatactaaa gggaaaaggt aaagtatttg
     5581 ataatgggga atttgtagaa gtaaatgagg gtgatgtagt gtatacagct gatggcgaaa
     5641 aacattctat agaaaatata ggagaagaag atcttgagtt tgtagcacta gttttatata
     5701 tgtaaaatat ggaggaggag tcatgacagg caagatagaa gaaattaagg acaagataaa
     5761 tagattttgc aactccaatt taaatcaaga atacaaagat ataagcttta aaattttgca
     5821 aaacctttta gataataata aagagataat tcattcttct agagctgata tatggtcttc
     5881 agctatacta aatatagttt tagaacaaaa tcttttatac aataagaagc atccactaca
     5941 tataacaaaa aaagagtttt caaaacaaat aggtgtttct ttaaatacta taaataataa
     6001 atcgtcttta ataaaagatg tttgtagtat tgacttttta aatcaagata ctatagctat
     6061 ttctaatgta gaattttggg taaaagagac caattccaaa tcaaagtctg tagatttaga
     6121 tttagaaaag aaaaaaatac tatataaaag atatattaag ctatcacaag atgcgaatag
     6181 tgatattgaa tcaattagat atatggagga agctgtaaat ataggtaaaa gtatgattac
     6241 taaagaagat aaagaacttg gattttggaa aggtttatct accagagcct atatgatagc
     6301 acttgaatcc cttgctagaa agttagaaag tattaataat ctaaaggagt ccaaaaaagt
     6361 attagaatat ttaatagaaa ttaatcctag tgatgagcag ggaataagat acaaattatt
     6421 aaatgtatta attagattaa atgatagaac ttcaataaat aaattatttg aactttataa
     6481 agaagaaaaa tctgctacat ggatgtacag taaagctctt tattacttta aaaataaaag
     6541 tatgtttttg gcaaatgatt ctattaaggt tgctagaagt aaaaataagt acgttggatt
     6601 atatttgtta gattggcgaa atgcttttgg tagagaattt gttacagagg aagaaaaggc
     6661 agaagctgtc tattactatg atgaaaacat agtggtttgg aatgaagtta aaggttctat
     6721 ggattggcta ttaaaaaaaa tgttggaatt ttcataaaaa aaactactcc ttacttttat
     6781 ataagtaaaa ggagtagttt ttaatttata catctagttt taaatcttca ggttttatta
     6841 gacctttttt tctcataaat tctgaaatca acaaagttac aatagcagga agtataaaat
     6901 taagtaaaag tataccaacc cacatagata tacctccttt tccagcactt tgcatagcag
     6961 ttactgttcc aaattgtcca accaatccac aagtacccat accagaacca ataggaatat
     7021 tctccatttt gaatattgtt gtagataatg gtccaagtaa tattgaagct acagtaggtg
     7081 gtatccaaat tttccaattt tttattatat taggtacttg caacatagat gtcccaagac
     7141 cttgtgccac aaggccacca actctatttt ctctaaagct cataacagca aagcctacca
     7201 tttggcaaca acaacctaca gttgctgcac caccagcaag accacctaaa cttaacatca
     7261 tacaaatagc tgcactactt ataggtaatg taagagctat tccaacaagt gcagagacta
     7321 aagctcccat tattataggt tgcatagttg tagcttgcat tattatattt ccaaatgaag
     7381 tcataaaagc agaaacagct ggaccaataa gttcagctac aagtactcca attagtattg
     7441 tcacagcagg tgtaaccaat atatctattt ttgtttcttt tgaaactaat tttccaacct
     7501 ctacagcaaa aagtgtagcg acaaatgctc caacaggtcc tcctaattca ttaccacaag
     7561 ctccaacaag tgcacaagaa aatagtacaa gtggaggtgc ttgaagtcca tacgcaatag
     7621 ccactgctat agctggacca gtaacctgtt tagcaagagg gcttataact tcagtaaaaa
     7681 gagaaatatt gaaattatct cctaatgtat tgaaaattgt tccaattaaa agggacgaaa
     7741 aaagaccaag tgccatataa cttagtgcgt caataagata cctttgaaca gaaatttcaa
     7801 tattttttct tttagcaaat gatttgaaat ttcttttttt ataattgatt tccaatttaa
     7861 aatcctcctc atataatata agtataattt ctttttaatt aattatataa cttttcagct
     7921 gacaagacaa atgttatttc actaaaatta gactattgta atagtaagaa attaacatta
     7981 attttagttt taaattttaa atttatggtt tataattagt atagggatat tctgaaatat
     8041 aataattttc cagtttatcc caatatttaa cttaggtaga ttgcttacaa aacaaatgat
     8101 tatatattag gctaaaagga agtggataga tgatatttgt aaaaaataag aaatacagaa
     8161 aaattgccat aattatatct attcctatat tgcttgttgc gcagtactac ctttttaaat
     8221 ttggaatact tagaccaatg gtaaagggag tagaaattga aattgtagat ggggaatatg
     8281 ttaaagatat agataaatat gtagtaaaat taaatgaaac agttactttg tctacaggtg
     8341 aatatattaa ggttccttcg tatgctaaaa atccaaatat ttggtttaat gtattggata
     8401 actcagggac tttaaaaata gatggaaata aaatgacagc attaaaggtt gggtattctt
     8461 ctgtgggtat catgaaaaat tcaagagtat taaaaaaggc tactataaaa gtagtagacc
     8521 cagaggtaga atcactagat atggagatta atggggattt aaaacatgtt ggtgatagtg
     8581 ctactataga aagtacaata gaagttgatt atgataagtt taaaaaatca tataagccag
     8641 tttataagag tagcaatgaa aatattttaa aaatcaaagg aaataaagta agtgcagttg
     8701 gagttggtaa agctacaata tatgctaagt gtggaaataa agaaattgag agaacattta
     8761 ggattcaagc tagagttgct gaaataaata tggatgatat aaaaatagaa gttgaccaaa
     8821 atgaaaaatt gaaaccagat ataataactt ctccacctgg tttagaacca ccaaaaatat
     8881 attatgagtt agtagattca aaattacctg tagaaagagc aataagttta agtagtaatg
     8941 gaaatgttgt aggtcttaga gaaggtaaag atagggtgag aataacttgt ggagataaat
     9001 ctaaagtaat aacagtaaca gtagtcaaag aaactataac gaatgactat atacaaaact
     9061 tggttggttc acataaaata gtaaacaata aagttattat taaacttgaa tgggattata
     9121 tgaaagatgt ctttgattat gatatatatc ttaaagataa gttgtcagga gaatcattta
     9181 agaaaattaa atctataact ataaatgaaa gtgatttagg taagtctaac aaggtatatg
     9241 caactataga agtagatatg aagggtaatc aaaatataga ttttgatatt tatgtcatag
     9301 ggaaaaagga ttcagataca actaagccaa gtaatgtaat aaatattact aatggaaatg
     9361 gaaattcaaa taatgataat aatattaaag atatgagggt agaaaatata tcagctaatg
     9421 ttgatagaga taataatgtc attagagtta actggagtag tatatctcat aaggactgta
     9481 cttacagtat atatgtaaaa aataatacta atggagatgg agaatttaat ctatatcaaa
     9541 gtgatataca gggaaatgag catactataa gtattcctga aggagattta aatctagata
     9601 tttatgttgt tgcaaattat aatggaaata gctctcaaaa tagtgatgtt ataaatgtaa
     9661 gatagatttg tatacaaatt gaaagtgttg agtttattta aatattaaat taagaaaaag
     9721 gatagtatag ggtaattata ctatcctttt tttgttacaa tatataggtt taaaatctct
     9781 agtaatataa attataaaaa gtcttattta cacaaatcta tagatatttt ttttattaat
     9841 caatattgtc ttaaattgaa cttagtgcgg cttctattgc atgtattact gtcgtatcaa
     9901 atagtggaat agaagtgtca ttttgtttaa ttaaaagacc tatttctgta catcctaata
     9961 taacaccctc tgcaccttgt ttgccaagct tatctataat agataagaaa gctttttttg
    10021 aagattcaga tataattcct aagcaaagtt cgttaaatat tgtgtcattt actattatac
    10081 ggtcttcttc atttggaatt aagacttcaa ttccattatt aataattaca tttttataaa
    10141 aatcttgttc cattgtatat ttagtaccta ataaggccac tttttttatt tctttttccc
    10201 ttaatactgt agcagttaca tctgctatgt gaagtaatgg aatatgtata ctttcttgta
    10261 ttttatctga taccttatgc atagtattag tacagataac aataaaatct gcccctgcct
    10321 cttgtaattt aattgcagca tcagctaaaa ttttggcgct tttttcccaa tttccactag
    10381 attggcattc ttcaatttct tgaaaatcta cactgtaaag tatacattta gaagagtgta
    10441 gtccacctaa tctttctttt attactgtat taataacttg ataatatgta atggtacttt
    10501 cccaactcat accaccaatt aagcctatag tcttcatatt atacctctct tatttattta
    10561 ttttgaaata gctatttaca aatacaagta tagcatctaa aatagaagca tacaataata
    10621 aaattgtatg gagtacaatt ataattaaga ggagtaatta tatgccaata aattcttttg
    10681 aaaactatcc tatgacatgg aaaccaaaat tagataaccg aaaaccacca atatataaaa
    10741 ctttagcaat gttattagaa gaagatatta aaagaggaaa tttaaatcct ggagataaac
    10801 tgcctccaca acgggaatta gcagattttc tagatttaaa cttaagtaca ataacacgag
    10861 catttaaact ttgcgaagaa aagggactaa tatgtgcaaa agtgggaaag ggaacattca
    10921 tttcttctga tgtaaatgta agtaatacat tattatatca aacggaatca aaagacataa
    10981 ttgaattagg gacagttcat cctccatatg agcaaaatac atatattatt gactttatta
    11041 aaaatgtact aaaacaacca gaaatggata ggtttttaca atacatgtct ccatctggca
    11101 catatatgca aaaaaagtca attgcaaaat ggatagaaag aaataatgta tacaccagtg
    11161 aagaaaatat attattatct acaggagggc aaaatgctat atgtgcaact ttactaggat
    11221 tatttaaagc tggtgataga attgctacag attctttaag tttttctgga attaagtcaa
    11281 tagctaaaat gataggtatt caattagtcc caattcctca agaaaataat gaaatatcga
    11341 ttgaatatct tgaaagttat tgtaaaaatg aaaatataaa aggcatatat cttataccag
    11401 attatcataa tccaacgaca catactatgt ctgatttatc tcgtaaaaag atagccaaga
    11461 tagcaaagca atataatata ataattattg aagatgctat aaatagcatt tttagagacg
    11521 gtatacaaac tcccatattt tctttagcta gtgataatac tatatatatt tttagtacat
    11581 ctaaattttt atgtgctggt cttagagtag catttgtagt tgctccaaaa cgatatatag
    11641 aaaatttaga aaatgcacta tataatatga atctaatggt ttctccattt acagcagaaa
    11701 tagtacatcg tttattatat tctcctatta tagataaaat tatagaagag aaaagagatg
    11761 caattataga aagaaatgaa gttgctgata aagttttgtc agattataat ttgattggtg
    11821 ataagaattg cagttttcgt tggttacttc ttcctgatga attggatgga aaatcatttg
    11881 aaatttctgc aaaaaatcta ggagttcaag tatattgtgc tgaacgattt tcagtaggaa
    11941 actctacagt tccaaaagca gttcgtattt gtgtaacagc tccaaaagat gtagaagaat
    12001 tagaaaaagg gcttaatata ataaagtcac ttcttaagag ttaaatttat tttactatta
    12061 tttaattaaa taaataaaaa ggcggagaaa tttattataa taaacctccg cttttattat
    12121 ttaatgaaat actttacttt ttattaaaaa atttaatttt aataatcttg aaaatataat
    12181 cgttttatgg taaaataatt cttaaaaata gaaaaaatta atcatttgtt aaaaaaaatg
    12241 cataatttat tgtaatttat tgacgaaggt tgaaaaatat tcataaatgt gcgaaataac
    12301 agaaaaaatg caaaaattaa ttcaatttca tttgttataa aatctatatg gcaaaattaa
    12361 atctgcctat cagaatgata taaacacatc agaaaggagg acgctatcaa ataatacaag
    12421 tagttaactt tgatagcaaa aaattatgaa tgaacaaact agaatatcct tagagagagc
    12481 tgctgaatta aaatcaaaaa ttgatgatta tattcaggct agaaaaacga ttaacagagg
    12541 tcttgaaaaa gaagaagaga taaataaacg aaaacagaaa atattaagta tcttaaatgg
    12601 aactgaagag gattggaata actacaaatg gcaattatca aatagaataa cagatgtaga
    12661 tactttatca aaaattataa ctctaactaa aaaagaaaaa gaatatataa aagaggttgg
    12721 tactcaattt agatgggcaa tatctccata ttatttgagt cttatagacc cagaagatat
    12781 atgtgaccca ataaaattac tgtctatacc aacacatata gagttggaag atgagcaaga
    12841 agatttggac ccaatgggag aagagtatac aaacccagca ggatgtataa ctagaagata
    12901 cccagataga ttaataataa atgtaacaaa tgagtgtgct atgtattgta gacactgtca
    12961 gagaagaaga aatattggac aacaagattc tcataagtca aaagctatta tccaagaatc
    13021 tatagactat atcagagaaa atgaagaaat aagagatgta ctagtaactg gtggagatgc
    13081 tcttacatta aaagatgatt atttagagtg gattcttagc caacttaaag agataccaca
    13141 tgttgattat gttagattag gtactaggac tcttgttaca atgccacaaa gaattacaga
    13201 tgaattttgc aatatgctaa aaaaatatca ccctgtatat ataaatactc attttaatca
    13261 tccaatggaa ataactaagg aatctaaaga agcttgtgaa aagttagcaa atgcaggagt
    13321 tccattagga aatcaggcag tattattaaa tggaataaat aatgataaat ttgtaatgag
    13381 atgtttaaat caagaattac taaaaataag agtaaaacct tattatatat tccaaagtaa
    13441 acatgtaaag ggaacaaaac atttcaatac atcagtagat gatggtcttg aaatcatgga
    13501 gtatttaaga ggatatacat caggaatggc tataccaaca tatatagtaa atgcgccaaa
    13561 aggaggagga aagactcctt tgcttccgca ataccttgta tcaaaaggaa cagattacgt
    13621 tatgcttaga acatgggaag gaaaagttat aaaaatggaa gatgaacctg ctgtagatat
    13681 aaagaaactt ataaaagagc aagcacagga ttaaaaaagt atttaaagtt tataaaaaga
    13741 aacaacttta aaataaaaag aaaatggatg tcaaatttat atcaatttgc atccattttt
    13801 tattagttat ttattatttt gttgttgatt aactaattca agatatccac ttgggtggaa
    13861 gtaatatatc tcagaagtat tttcatttac aaagtaacca atatcaccat ctgcatctgg
    13921 taaaaataca taaccattaa gatttttagc ttttaatgct tcaaaatcac tagcatttcc
    13981 ttgatatatg taatcaactt cattgttgta ttttactaaa agttctttag cttgttcttg
    14041 agtcaattta gtattttggc ttacaggaac attcttttga gaatctgcat aagaagattc
    14101 atttactggt gcagttaata acaaacaaga taaaactaat aaagatataa gtgctttttt
    14161 catactaaaa ccctctttca tgtttaatta tataagattc attacttaat aatattgtat
    14221 gtttcctata attaaatatt accattaaag agaataatat tacagttaca cagctgtaac
    14281 ttttgattac aaaatcgtaa ttaaattgta tttttattcc atttgtattt tatcatgtct
    14341 acgatttaca aactcacaga agtatatctt tataacgccc atcacaggag cagatagaat
    14401 cataccaggt ataccaaaga atcctccacc aacagtaact gctaggattg taaagaatgg
    14461 gctaagacct agttgtccac ctacgatttt tggctctata aatgtaactt caacttgttg
    14521 aattattatt aagtaaatta aagaagttaa agcaattgtt ggattggaga ataaattaat
    14581 tattacaact ggtgccatac ctattactgg accaaaatat ggaaccatat tcatgacacc
    14641 cataagtgta ccaaataata gtgcatattc agattttaaa aagtaaagac caattccaga
    14701 aagtactcca actataaaag aatctaaaat cttacctgta aagtatttac caatatttaa
    14761 atttaagctt tgacatagtt ctattataaa gtctccatat tttttaccta gggaaatata
    14821 aactactttt tttgtaaaca gtaagaattt ttctttttct aataatatat agaaacaaat
    14881 tataaaacct aaaagaaatt gtactataaa ttttccaact gaaaatgtag tgctaaatat
    14941 cccaccaagt gaaccaataa gcaagttact aaatttaggc attacactca taattttgtc
    15001 accatactct tttaatgtat tagggtcaac tgattgtaaa ctcttaccta agtcgaataa
    15061 gaactgctct gttttatcta tgtatgtagg aatttgagca actaaatcag ctaaactatt
    15121 tactatggat ggaactgtaa ataatataaa ggaaattagt ataactatga gtacaccata
    15181 agtaagaagt aacgaatata ttctcttaaa atttaattta ctttctaaga actttactag
    15241 tgggttgaat atatatgcta atacaaatgc tattataaat ggtgttagta gtgacattaa
    15301 taaacttatc actccaaaaa aatatttgta attatcaata actttaatca agatatatga
    15361 aacaactaca gtgagtacaa tatttagata aattttttta tcttgcaaaa taacacaacc
    15421 tttcttatat ttattaaatt tagatatact taaatctaag ttaaagttaa tatttatagc
    15481 ttattataca ataaaaagca gtattaatat agtatgatta atatgaaatt ataaatacgt
    15541 taaaacttta tgcttatagc taaaataata aaaattattt atattatgtt tatatataat
    15601 ctaaaatgaa aaaaagtggt gcctttgatt aaactaagtt tttataattt aaattttgta
    15661 caataagcta tcttttatta ttctttaaaa tatatatttc atttaattat gacaaataaa
    15721 gataaaaatt aaatatttta gaggtgattt tatttagata tagaatttta gaataatact
    15781 atacaaatgt attgtattat tatttttata tcaaaaatag taataaagtc tatatacatt
    15841 tagaaaatac tgtttaaaag gagtaggatt tatgataaaa aaagagatat ctaactttag
    15901 aggaagtagt gattatgtag tatcaccaga attgatggca tcagttaatg ttgctattgc
    15961 tcttgaaaag ccacttttga ttaagggcga acctggaaca ggtaaaacta tgctagcaca
    16021 agcaatttca aatgaattaa aaaaagattt agtaatttgg aatataaaat caactacaaa
    16081 agcacaagaa gggctttatg tatatgatac tgttcaaaga ctttatgata gccaatttgg
    16141 aggagaaggt gtagatgata ttagcaagta cataaaatat ggtaaactag gagaggcctt
    16201 tagctcaaat caacaagtaa ttcttttaat agatgaaata gataaggcgg atttagaatt
    16261 tccaaatgac ttgctttggg aattggataa aatggagttt tacataaatg agacaaaaga
    16321 aactgtaaga gctaaacaaa gaccaatagt tattataact tcaaatgcgg aaaaagaact
    16381 tccagatgct tttttaagaa gatgtatatt tcattatata gaatttcctg atagagacat
    16441 gatggaagaa atagttaaag tacattttga taaagtagaa gaacacctat tggaacaagt
    16501 aatgactact ttttattgga ttagaagttt aaaggacata caaaaaaagc ctagtacatc
    16561 agagttaata gactggatac aagcattaac tttgagtgga atgcctattg aaaaaataga
    16621 aaaagaagtt ccttttgcag gaatactact taagaataat gaggatattg aatctatgca
    16681 aagacactta tagttaatag gagctggata attatgttta ttgaattttt ttatttattg
    16741 aggtcaagag gattaaatat atctttaaat gaatggatga ctttaattga agcattggat
    16801 aaagggctgt gctattcaaa cttttcaaat ttttactatc tttgcaggat gattttaata
    16861 aaaagtgaaa gtgattttga taagtttgat gcagtgtttt tggagtattt taaaggtata
    16921 gaacaccaag aagaaattcc agaagagatt atgaattggc ttaaaaaacc agatatagat
    16981 ttagaagagt ttgaaagatt ggaacaaaat ccaaatataa atcttgataa attaagagct
    17041 aaattggagg aacgaataaa agaacaagac tctcctcata atggtggtaa ttattggata
    17101 ggcacaggag gcacttccga gttaggacat tcaggaaaag ggcaaactgg tatacgtata
    17161 ggtggaaatt ctacttatgg tagagctgtt cttgaagttg caggagaaag aaaatacaga
    17221 gattttagag atgatgaagt tttaaatatg cgtcaatttc aagttgcact aaagagactt
    17281 cgtcaatttt ctactagaat agatgctcca aagacagagc ttgatttgga taagactatt
    17341 gaagagactt gtaataatgc aggatattta aagttatttt ttgagaagcc aagaaaaaat
    17401 acagtgaagc ttttattgct tatggattca ggtggttcaa tgagggggta tagcacttta
    17461 tgtaatacat tatttcagtc tgtaagtaaa tcaaatcatt ttaaagatgt aaagatatat
    17521 tatttccata actgtattta tgatagatta tttacaacac ctgaatgttg gttaagtaaa
    17581 tctattaata ctgaatggat attaaaaaat atagataaaa actataaagt tataattgtt
    17641 ggagatgctt ctatgtcgcc ttctgagtta cttcatattg gtggtaacta tcgtggtcca
    17701 tataattaca cacctggtat agaatggctt aaaaggttta agagaaagta cagtaaaatt
    17761 gtatggatga atccagaact tagagatgga tgggattcaa ttaattattg gtaccaaact
    17821 caaagaatga tacaaagtga atttaatatg tttccactga ctgtaaaagg tcttgaaaaa
    17881 gcattgaaaa atttaatgct ttcaagataa agtaaatata aatgatggta tgatttattc
    17941 aaaattgtac catcattatt tattttttaa tatatctatt ataaattgta taactgaaac
    18001 ttaaaaaatt atatagtcta atacatttta gacttacaca aaacatatac attaataata
    18061 attttagaag tcgaggtgat tataataaat tatgaattaa ttgtaaaata caatggagat
    18121 atattgagat tagaagaaga attaggtgta tcagtagaga tacttaattc atcatatgca
    18181 ataataacat caagtaatga agaagatgta aatacacttt taacttatcc agaaattgaa
    18241 tttatagaaa agccttttat attacagact caggatgtgc aaagtttttc aagtacagga
    18301 ataacggggt ttaaaaatag aactggttta actggaaagg gaactataat tggtataatt
    18361 gattcgggaa tagattatac tctacctgtg tttagagata gtgatgggag gtctaagata
    18421 ttatattatt gggaccaatc tattcaagga aatccaccag aaggttttag agaaggaaca
    18481 ttatatacta atgaagatat aaataatgca atagatggaa gtatgtatat acctatttca
    18541 acaactagtt tacatgggac acatgttgca ggtatttgtg caactattgc aagtgatgcc
    18601 agaataatag tggttagagt tggaaatata cagaccgata ttttttcgag aagtacagag
    18661 tttatgaggg caattaaatt tattttagat agggcattag aattaagaat gcctgttaca
    18721 ttaaatataa gttatggaag taatgaaggt tcacatagag gaacatcttt atttgaacaa
    18781 tatatagatg atatgtgttt attttggaaa aacaatatag ttgtagcagc tggaaataat
    18841 gcagataaag gaggtcataa aaggattagg cttcaaaata atattacaga agaagttgag
    18901 tttatagttg gggaaggaga gcgtatatta aacataaata tatggccaga ttttgttgat
    18961 gattttagtg tacatttggt aaacccatca aacaatcaaa cacaagcaat ctctctaact
    19021 tcaggtgaga taagaaatac tttgggtgaa actagaataa ctggttattt ttaccctata
    19081 gcaccgtact ctcttacaag aagagttact ctacaattaa gttcaaatac acagataact
    19141 cctgggttat ggaaaattgt atttgaacca atagatatag taactggtaa tgtaaatata
    19201 tatttgccaa cttctgaagg actaaataga aatactagat ttctgattcc tactcaagag
    19261 cttactgtaa cagttcctgg aacagcaagt agagttgtaa cagtaggtag ttttaattca
    19321 agaacagaca tagtatctat attttctggt gaaggagata cacaattagg tgtttttaaa
    19381 cctgatttat tagctccagg tgaagatata atatcttttt taccaggagg tacaagtgga
    19441 gcattaactg gaactagcat ggctacacct catgttacag gggtttgttc attgtttatg
    19501 gaatggggaa ttgtaaatgg aaatgattta tttttgtatt cacagaaatt aagggcatta
    19561 cttttaaagg gagcaaggag attaagtaat caatcttatc caaataattc atcaggattt
    19621 ggttttttaa atttatcaga tatagatttg tacactctat ctagtataaa tcaagattta
    19681 gaaacggagg atattggata tagaagtatt aataagtcat ttaaagatga ggaaaatagg
    19741 tataagttta tagatggtta taatatacaa atacataatg acttagagaa tgaaatttat
    19801 ataagtaaaa atgcaagtag acaatcagga atactatcag gaatagatgt agtccatact
    19861 cccgaatttg aagaagaact agctgggtta ggtatgagtc aaaatttttt taagataagt
    19921 gattcattag gtgtactatc tataaataat acagactata gtagtataca acgagttttg
    19981 caacttcctt caatcataag aactgtatcg actacaaaga tgacattact tggagaaatt
    20041 aatcgaggta cttttggagg tgtagttgca acagaagaaa tgggcgtaaa tttctttaaa
    20101 aataatccta atattaatat aacaggaaga gggactctaa tatcaattgc agatacagga
    20161 atagactatc tacatccaga ttttatttat ccagatggta cttcaaaaat agtatattta
    20221 tgggaccaaa caaaagaagg aactccacct gatggttttt atattgggac agaatataca
    20281 cgagaagata taaatcgagc tatcgcagaa aatgacccta gtctatctca ggatgaagtt
    20341 ggtcaaggaa ctatgttgag tggtatatgc tcagggcttg gaaatgtaaa tagtgaatat
    20401 gcaggtatag cagaagattc tgagctaata ataataaaac tcggtaagat agatggtttt
    20461 tataatagtg caatgttgtt tgctgcatct cagtatgcat ataaaaaagc ttttgaactt
    20521 agaagaccac tagttataaa tatgtcttta ggtaccagta gcttggcagg tttaaccaat
    20581 aggagcaata gtgaaaaagc attctttact agagggttat gtataacagc aggagcagga
    20641 aatgagggca atacacagac acatacatct ggaataatac cacatgttgg aggttctgtt
    20701 gaagttgaat tagagttaaa tgaagacgag gaagaattat cattagaact ttggttaaat
    20761 agaccagata aagctgatgt aataatagta tctcccactg gtgaagaaag taaaagtgtt
    20821 ggaatttcaa attataataa agtaacaggt ttatttgatt tagaggggac tgaatattct
    20881 ataacgtata tatacccaac aactttttct gggcagcaat ttactaatgt tactttaaag
    20941 aatgcaaaaa gaggtgtatg gaagattcga ttagtagggg tttatattat aacaggaaga
    21001 tataacttat accttccaaa tagagaactt ttaaaaagtg gaactagatt tagagaggta
    21061 gacccttttt acactataaa ttatcctgct attcaagatg atttaataac tgtaggagcc
    21121 tacaatacta ttaatggtag tttatggcaa agttcatcaa gaggaccaac tattgaagac
    21181 aggttaaaac cagatatagt agcaccaggt gtcaatataa tagctgctta tcctgggaac
    21241 acgtatgcaa ctataactgg tacagcagca gcaagtgcac atgcagctgg tgctgctgca
    21301 atgtatttcc aatatacttt tgttgatggt agatatccaa accaagcata tgtgcaaaaa
    21361 ataaaaactt ttatgcaagc tggtgctaga aaagattcaa acacagtata tccaaataca
    21421 aattctggat atgggctttt ggatgttaga ggaatgtttg atgttttaag ataggaggga
    21481 tttatggaaa aatcttattg tataatttat caaggtgata tagaatctgc cctacaagaa
    21541 aatggaataa ataggtatat ggttttaaat agtcagcttg cagtaatata tgtacctgta
    21601 gattttgatg aaactatttt aaataatata atacaagttg cttggtggga agaatctgaa
    21661 ccaatgagca gtctaattga aataactaat aatgtaaata atggagaaac cattacaaca
    21721 gcagctgaaa ctgattatat ttatgaaaat ccatataatg atataacagg aagaggaatt
    21781 ttattagcag ttatagattc tggtatagat tatttgcatc ctgattttat aaatgatgat
    21841 ggaactagta aagttctata tttatgggac caagaagcaa atacaaatcc acctccagaa
    21901 ggttttattt ttggaagtga atttacaaga agtcagttaa atatagccat aaatagaaat
    21961 gatggaagtt tgagtcaaga taatataggt actggaactt tagtttctgg tatattagct
    22021 ggaaatggaa ggataaattc gcagtataga ggtattacta cagaatcaga cttaatagta
    22081 gttaaattaa aatcatatac agatacttat tatgctggaa ggataaatta tagtgtttca
    22141 gattttttag cagctataac gtatgttact aatatagcaa gaacagaaaa taaaccacta
    22201 ataataaatt tgactattgg agttaagtca agtgctgtag caactacttc aatattagat
    22261 acttttaata tattgagtag tgctggtgtg gtagtagtga gtggtgcagg aaatcaaggg
    22321 aatacagata ttcattattc tggcagattt agcagtgtgg gagaagttca agatgtaata
    22381 atacaagatg gagatgacta tgctcttgat ataacactaa atacgaatgg tccagataaa
    22441 gtgggagcac agataatatc tccttcaggt gaagttagtc atgatataag gtattctcca
    22501 gacttttata tatatagagg aaaatttaac ttagaaaata ctacttatgc aatgagattt
    22561 atttatccat atattacctc tggaaaagaa aatttggaga taagattaag agatataaaa
    22621 cctggagtat ggattttaag attgacttca gaacttataa taagtggaga atatgacata
    22681 tacttaccaa ataaaaattt gatagctcca gacacaaggt ttttagaccc tgattcagta
    22741 gctactataa ctatgtatgc tgctagtgat gatgtaatta cagttggtac atttaataat
    22801 aagactgata gtatgtggat tggttcttcg aaaggaccaa ttagaggaag aggaattaag
    22861 ccagatatag tagcttcagg tgtagatata atatccacat ataaaaatgg aacatataat
    22921 acaggaacag gaacaggagt gagcagttct atagtcacag gtgttttagc cttattgatg
    22981 gaatacttag aaaaacagga taatgtaccg agattatcat tgtttacaca agttttaaaa
    23041 acatatttaa tattgggtgc tacaaaactt gagatatata catatccaaa tgtatcgcag
    23101 ggatatggga ttttaaattt aaaaaatacg attcaacaga tagcaaatac tctatagata
    23161 agaacctatg taaaattata attgattagt aatttaattg tatcaaatac tttagataga
    23221 gtagaaaaat ggcataaaaa ataaatatga aaattaaaaa aacagtttta tattttgaat
    23281 acacaaagta aatattttgt atataaatag gtataaatta agtattgaaa agatatgatg
    23341 ttaatttaaa aatcattata atttagactt atgaatttta tttgtaaaat tgctaaaact
    23401 attacaaaat aaataataga ggtgatagtt ttggaaagta aaaaggcaag agcattaaaa
    23461 ggtaaagata ataaaagact taaaagttca cttttagttg gaaatgagtc gacagcagca
    23521 tgggctgatg ttgaaaaatt aaaacctcat agtaaagttt caataccttc aattaagggt
    23581 gtcgaagaag caaaagactg ggttgacaat ggaagtaaat tataaaattt tggaaattta
    23641 agtaataaag aagtaatgaa gataagtatt tattatcatt atgcaaggta gaattattat
    23701 attttaaata acaatcagta tcaattatat aaatttattg actaaaaatt tataaattgg
    23761 tataattttg ttaattagta gaaataataa aagataaata ctgtgaagag aagagtagat
    23821 ataaaatgta gttctagcga gttgaggatg gtgaaagctc aatacgaggt ttatattgaa
    23881 gaacatcttg gagtttctaa tcgaaagctt atttagtaag tagacttaga cggaaggcac
    23941 cgttataggc tttaagcatt gcttaactaa gaggtcatta tgacaactag ggtggtaccg
    24001 cgagagtaat agtctttcgt ccctttatat agggatgaag ggcttttttt attgaaatta
    24061 tatttaaaag aaattaataa caattatact aggaggattt aaaatgcaaa aggaatttat
    24121 aacagtaaaa gaattgtaca gagaaaaaga acagtacata gggaagactg taaaagtagc
    24181 tggatggata agaacatcta gagtatctaa aaattttgga tttatagagc taaatgatgg
    24241 aagcttcttt aaaaatatgc agatagtatt tgatgagaaa gtagaaaatt ttaaagaggt
    24301 aggaaaatta gcaataagct catctatact tgtagaggga gagttagtat ctacagaagg
    24361 ggctaaacaa ccagtcgaaa tacatgcaaa aagcatagtt gtagaagggg aatcagatag
    24421 ttcgtatcca ctacaaaaga agagacatac gcttgaatat ttaagaacga tagcacattt
    24481 aagaccaaga agtaatactt tttcagctgt atttagagta agatcaatag ctgcctatgc
    24541 aatacacaaa ttcttccaag aaagaaactt tgtatatgca cattctccaa tcataacggg
    24601 tagtgactgt gaaggtgcag gagaaatgtt tagaataaca actatggatt taaatgatat
    24661 tccaaaaact gaagatggta aaatagacta tactcaagat ttctttggaa aagaggctaa
    24721 tttaacagtt agtggtcaac taaatgccga gataatggca ctttctttta gaaacgttta
    24781 tacttttgga cctacattta gagctgaaaa ctcttataca ggaagacatg cttcagaatt
    24841 ttggatgata gagccagaaa tagtttttgc agaccttgaa gataacatgg aacttgcaga
    24901 agatatgata aaatatgtta taagctatgt attagaaaat gctcctgaag aaatggaatt
    24961 ttttaatagt tttatagata aaggattgtt agaaagatta aataagatag taaactctga
    25021 gtttattaga ataacgtata ctaaagcagt ggaattatta attgaatctg gtcatgaatt
    25081 tgagtatcct gttgagtggg gatgtgacct tcaaactgag catgaaagat atataacgga
    25141 gcaaatatat aattgtccag tgtttgttac agattatcca aaagatataa aagctttcta
    25201 tatgagaatg aatgaagacg gtaaaactgt tagagcaatg gatttattag ttcctggagt
    25261 tggagaaatc gtaggaggtt ctcaaagaga agaaagagaa gatgtattgt tagatagaat
    25321 taatgaaatg ggattaagag aagaagatta ttggtggtat ttagaactta gaaagtttgg
    25381 tactgctact cactctggat ttggtcttgg atttgaaaga ataattatgt atatgacagg
    25441 tatgtctaac ataagagacg ttataccttt cccaagaact cctaaaaatg ctgaatttta
    25501 ataattatat ataataaaac ttatataact ttaagtataa acactaaaat gacttgcata
    25561 tttatctatt ttagagatat gcaagttatt tttattgtac tataaatata taatttgatt
    25621 acttttattt tgcgatataa tacttatagg tttatataat agtatgtaac tttttaataa
    25681 aatgcaacac aattacgtcc ctgttctttt gcagtatata gagccttatc acaattactt
    25741 ataaactcat ttaatgttat tgccttttca attttctttg tataaactcc aatactaaca
    25801 gttacacatt ttaaaggaac atcatcacgc tcaaaattgc aggactctat aatttttcgt
    25861 aaacattctg atttaagttt agcatctgtc tcatcacagt taaaaataca tataaaaaat
    25921 tcctcaccac catatcgtat agcatgttca ttttcagaac ttgtaaaatc taaaattact
    25981 ttggcaatat tttgtagaca atagtctccc ttttgatgtc cataattatc attaaattgt
    26041 ttgaaataat ctatatctat catcattcca gttataggtt cttgatttgc atggctgatt
    26101 aatttttttt gtagaatatc ttcaaggtat ctacggttgt tcattttagt aaggtcgtct
    26161 gtcataactt tgtcgttaag taaattattt atatgagata tttcttcata ttgttgacta
    26221 attaaaagtt tatcatagta agtaaataca cgatttctat atagcattgt agaaacaaaa
    26281 atggcaaggg atgttataaa aaatgtgttg acaatattac taaactcatt gcctggtata
    26341 gtttgaatcc caggtaagaa taaattaaga aacaaaaagc atcctccaaa aactattact
    26401 gattctccaa attttaatac tgagaaagct gcagctgcta acataatata actaaataca
    26461 cttaggtcat ttccacctaa ctggtcaagt agagtaatta cacaacccca taggcagaaa
    26521 aaagtcacat aaattatcca taaatttata taaaacgtac aacaatcctt ttttttattc
    26581 caaagataag aaaatacaaa cataaagaca gaagatacac aaaatagtgt tacatacata
    26641 taaaaatacc attggcgtct tggtgtggca aaagggcctc cttctctagc tgagattgat
    26701 atcatcataa ttatttgaga tattactatt attatgcaaa tgattttacc cgtatgataa
    26761 agataattgc agagttcttt ttcaacattt tctttgtgtt ttttgtctag aggaaatcca
    26821 aaaaattttt ttattttttc taagttcata taagcctcca ttaaaaataa agcataaaaa
    26881 taagtattcc tttctatcat tagatatttg gaatacttcg tttatgattt aattatatca
    26941 aaatatattg tgtgtaacaa tctaaaattg tgatagataa aatttattag ctaatgttga
    27001 gtaactttat taattaatac tttactataa ttaagattta cagatagttt gctgctgaaa
    27061 ataaagaata gaataaatt
//
LOCUS       DAGQWR010000048        26554 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.048, whole genome shotgun sequence.
ACCESSION   DAGQWR010000048 DAGQWR010000000
VERSION     DAGQWR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.048"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1108)
                     /locus_tag="MIW95_001724"
     CDS             complement(<1..1108)
                     /locus_tag="MIW95_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151053.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQDIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFML"
     gene            complement(1120..1944)
                     /locus_tag="MIW95_001725"
     CDS             complement(1120..1944)
                     /locus_tag="MIW95_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151054.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQNILFFGMLGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIVLITMIYGFLISQTATLFICKYIFNFIFRNIPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIINLFRKYYNLIGFQFNNLVAGLLSFLNIMFILNGISYLLIKKKVSL"
     gene            complement(1937..2653)
                     /locus_tag="MIW95_001726"
     CDS             complement(1937..2653)
                     /locus_tag="MIW95_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151055.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            complement(2725..3081)
                     /locus_tag="MIW95_001727"
     CDS             complement(2725..3081)
                     /locus_tag="MIW95_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY3151056.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            3437..3895
                     /locus_tag="MIW95_001728"
     CDS             3437..3895
                     /locus_tag="MIW95_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBY3151057.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            3899..4861
                     /locus_tag="MIW95_001729"
     CDS             3899..4861
                     /locus_tag="MIW95_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151058.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVKMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            4855..5604
                     /locus_tag="MIW95_001730"
     CDS             4855..5604
                     /locus_tag="MIW95_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151059.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(5670..5972)
                     /locus_tag="MIW95_001731"
     CDS             complement(5670..5972)
                     /locus_tag="MIW95_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3151060.1"
                     /translation="MIKILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRVPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(6111..6743)
                     /gene="thiE"
                     /locus_tag="MIW95_001732"
     CDS             complement(6111..6743)
                     /gene="thiE"
                     /locus_tag="MIW95_001732"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBY3151061.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITERYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEATCAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            complement(6766..7554)
                     /gene="thiM"
                     /locus_tag="MIW95_001733"
     CDS             complement(6766..7554)
                     /gene="thiM"
                     /locus_tag="MIW95_001733"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBY3151062.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGIYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            complement(7578..8372)
                     /gene="thiD"
                     /locus_tag="MIW95_001734"
     CDS             complement(7578..8372)
                     /gene="thiD"
                     /locus_tag="MIW95_001734"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBY3151063.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     regulatory      complement(8472..8564)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8657..8968)
                     /locus_tag="MIW95_001735"
     CDS             complement(8657..8968)
                     /locus_tag="MIW95_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBY3151064.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     gene            9259..10503
                     /locus_tag="MIW95_001736"
     CDS             9259..10503
                     /locus_tag="MIW95_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151065.1"
                     /translation="MENNLNIAKKNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYYMENNTNITIYENNTLQYKFDEESEDIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKSRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            10700..11569
                     /locus_tag="MIW95_001737"
     CDS             10700..11569
                     /locus_tag="MIW95_001737"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBY3151066.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDMNKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(11623..11811)
                     /locus_tag="MIW95_001738"
     CDS             complement(11623..11811)
                     /locus_tag="MIW95_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBY3151067.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(11971..12561)
                     /gene="cysE"
                     /locus_tag="MIW95_001739"
     CDS             complement(11971..12561)
                     /gene="cysE"
                     /locus_tag="MIW95_001739"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBY3151068.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            complement(12588..13496)
                     /gene="cysK"
                     /locus_tag="MIW95_001740"
     CDS             complement(12588..13496)
                     /gene="cysK"
                     /locus_tag="MIW95_001740"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBY3151069.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            complement(13733..14359)
                     /locus_tag="MIW95_001741"
     CDS             complement(13733..14359)
                     /locus_tag="MIW95_001741"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBY3151070.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNASYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNIEVAKILGLI"
     gene            complement(14378..16435)
                     /gene="kdpB"
                     /locus_tag="MIW95_001742"
     CDS             complement(14378..16435)
                     /gene="kdpB"
                     /locus_tag="MIW95_001742"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBY3151071.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNVGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMIL
                     NLG"
     gene            complement(16460..18142)
                     /gene="kdpA"
                     /locus_tag="MIW95_001743"
     CDS             complement(16460..18142)
                     /gene="kdpA"
                     /locus_tag="MIW95_001743"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBY3151072.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            complement(18183..18275)
                     /gene="kdpF"
                     /locus_tag="MIW95_001744"
     CDS             complement(18183..18275)
                     /gene="kdpF"
                     /locus_tag="MIW95_001744"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBY3151073.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            complement(18686..19318)
                     /locus_tag="MIW95_001745"
     CDS             complement(18686..19318)
                     /locus_tag="MIW95_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY3151074.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            complement(19690..20652)
                     /locus_tag="MIW95_001746"
     CDS             complement(19690..20652)
                     /locus_tag="MIW95_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBY3151075.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            complement(20673..21623)
                     /locus_tag="MIW95_001747"
     CDS             complement(20673..21623)
                     /locus_tag="MIW95_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151076.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            complement(21646..23142)
                     /locus_tag="MIW95_001748"
     CDS             complement(21646..23142)
                     /locus_tag="MIW95_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBY3151077.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNSHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHTVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSEGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            complement(23155..24339)
                     /locus_tag="MIW95_001749"
     CDS             complement(23155..24339)
                     /locus_tag="MIW95_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151078.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKKSLMREMNNVSKGVIDNEDTPSGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKSLLEISSYFKSKGLKIGIDTFSPFISYFTGQDLKKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            complement(24332..25282)
                     /locus_tag="MIW95_001750"
     CDS             complement(24332..25282)
                     /locus_tag="MIW95_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBY3151079.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGLKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKIDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            complement(25284..26378)
                     /locus_tag="MIW95_001751"
     CDS             complement(25284..26378)
                     /locus_tag="MIW95_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBY3151080.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LRERSINGVISMCLKETDYQYSLSNKKGGY"
BASE COUNT         8847 a         4617 c         2897 g        10193 t
ORIGIN      
        1 atagcataaa tttattatcc caagataata tagagtttag ccttgatgta tatgcaacga
       61 ttttatttga atcaatttca atacttctaa ttcctctttt ttctctatta tctaaaacat
      121 atttatcttc atctaagctt ttatctaata tattataggt aagtaaattt aactctgtaa
      181 ttgaactatt atcataatca ttcgacatta ccatatataa tttattagct tctaactttg
      241 cttttgctaa agccattttg ctatcagttt ttaattccat tatgctttcc ttattttctt
      301 caagattata ctcaagtaaa tgaaatttag aattactatt atcacctatg tttgtattta
      361 gtacatttac taaaatatac atcttatcct catatttgct agcataactt acacctatgt
      421 tctctatgtc ttggtatttc tcggaaaaat aattacttaa atttatactt tttatagtct
      481 tgattttttt attttctgga ttaattttta ttatatctat agtacttgaa tattctttgt
      541 tattttctga tgaatcactt gtagcataaa gtacagatac atataagtat tttttatatc
      601 tttttgatgc tagtatattt gcataataat catctgtact tttatctact ttatatcttg
      661 tccttgtaaa attattcata tctaattttt catattcaat ttctttatca ctaccttctg
      721 atggaaatac acctttttct aaatcttctt ttttctcatc tatcacaaag tttatatctt
      781 ttgactcctc attcatttca aatccaacat gagcaattaa atctgaacca ctagatacat
      841 caattaaatt atttgtgttc ttactatcct tttgtagttt tattaattca gtctttttag
      901 ttttgttact tccatttatt gtataatatg aatttatata tccattcaaa gcatatccaa
      961 aatttatgtt tatatggtct aattcattag agtcaccttt tatagttttt atctttaaat
     1021 tatcttcatt atttaataaa cctatactaa aatatattgc taaagtcaaa aatataataa
     1081 gtatagatat tttagatttt aatttcatac cacccctcct taaagagata ctttcttttt
     1141 aattagcaaa taagatattc catttagaat aaacattata tttaaaaaac ttaataaccc
     1201 tgctactaaa ttgttaaatt gaaatcctat aagattataa tattttctaa ataaattgat
     1261 tatatttata tatattgttg aaaaatataa gcctgctata atcagcaaag tgattaaaat
     1321 tacataaaat ttatttatat aggaaaagat aaataatata cagcaattta caatagaaat
     1381 agctataagt agtaaaaata cataaatagc cataaaagag ataaaatcaa ttggtatact
     1441 cttcaagttt aaataatata aatctttggc aaagttcata ttgattatag gaatatttct
     1501 aaaaatgaaa ttaaatatat atttacatat aaataatgtt gctgtctgac ttatcaaaaa
     1561 accatatatc attgttatca aaactattga tttagatata caaatcttaa acttattttg
     1621 aggtaacatc ataagtgtat aaatactttg attatttgaa aagaactctc ttttccacat
     1681 aaaaatatag tatataaaaa gtcccaacat tccaaagaac aatatatttt ggataaaacc
     1741 aagattacta agtatcccta gtccataaaa tatacctcca aaatcttcta ttgctaagtt
     1801 cataataaat ttagaatttt taaatttatt aatttcgaat aaatttaaac taagtgtaat
     1861 taatgaaaaa aatgatatat aagctatata aaacttattg cttcttctca aatctacatt
     1921 tattagatat agaagtttat tcattcttat atacctccct catcttatct aatatagatt
     1981 taccttctct ttctcttaat tcttctgcat taaatgaagt tataatctgc ccttcatcaa
     2041 gaattacaac ctcatcaact aaactttcta tctctactat ctcatgagta gttatgataa
     2101 tggcttgatt ttcctccata tattgcgcta ttactcctat aaactcttcc ctcttaaaca
     2161 aatcaattcc tgtaaatggt tcatcaagaa gtatgtactc tgggtcttgg caaaatccaa
     2221 gtattaattt tactcttgca atgtttcctt ttgacaagtt atcaattatt tcatcttcat
     2281 ccaatttaaa tatgtccatc atttcttttg atttttcttt attccacttt gaataaaaaa
     2341 tttccataaa ttcaaatgtt tcttttattg ttgtattgct aaagtgagat tgtacatctg
     2401 gaacaaaagc caatttattg tatacctttt catttatttt ttctccatct atacgaattt
     2461 ctccactatc aatgtttata agtcctgcta ttgcttttaa catagttgat ttaccaacac
     2521 catttatacc aagtaatgca gttatttttc ccttttctat attaaaactt acatttttta
     2581 aagcttctat ctttcttatt ttaagccctt gcatcctttt atatgttttg ctgacattct
     2641 ttatttctat catatttact atcctccaca tccaatatgc ttataaataa actttctaaa
     2701 ctaagttttc tatttgttaa aaaactaata cttatcttct attattttta aaacttcttc
     2761 tttgctaaca tttattgctt tcatatcttc tataaaaaca gatagtgatt catttataag
     2821 ctctaatttt aaatttttta atatattttc atcaactgtt acactgcttt gataattttt
     2881 aaaagtattt attatattca tatcacccat ctccttatat gctctttgaa ctgtatttaa
     2941 gtttactttt agattaagtg ccatttctct tcttgatggt attgtctctc ctggtttcaa
     3001 ctctcctgtt actatttgtc tctttatata ctttactatt tgcaaataaa tgggagttgt
     3061 attatctaat tcaaaattca tgattcctcc tattctagtg tattaatacg atagtacact
     3121 tactttaaaa aaatacgcct ttatttcaga ctttatctag tatcctatta agtgtattat
     3181 acgattaata cacttaattt gtaaatactt attttataaa ttgctttatt tcaatttgct
     3241 aaaaaatcta aagtaaagct taaataatag ctttacaaaa tggagcatca aatgagagga
     3301 tatatatttt atgttatgtt agacttgaaa acaagcaaac gagataagat tatatgtttt
     3361 caactaatca aaatcttata tgtattaatg cttaaaaatc tagtaagaat atgaacacac
     3421 attaggaggt aaacaaatga attgcaaaat tgaaatcaaa aatatcgaat cagtcacagt
     3481 ggctaccatg cgttataatg gacctatgac tgaggcaaca aagtattttc caaatgtatt
     3541 taaagctata aaaggaaagt ctaacggtgc accattcttt tgctactatg atgttgacca
     3601 aaaaactggt gttgcagaaa tggaattatg tgttccaact gctgaaactc caaatcgtat
     3661 gggtataact acaaaagaat ttcctaaaac aaaagcttta ttttttactc acacaggctc
     3721 ttatagtaat ttgccaaaaa cgtatgaaat gatatttaaa tatattcatg aaaataatat
     3781 aaaaatacaa actccttgga gagaagtatt tattaaagga ccaggtatgt tgataaaagg
     3841 aaacccagat aactatatta cagagataat atttccactt aaagaggagg aataatatat
     3901 gttagcgatt gaaatagaca atttagtaaa agaatataaa aatggtgtga aagctttaaa
     3961 tggtctaagc tttaatgtaa atgctggaga aattttttct ttacttggtc ctaatggtgc
     4021 tggtaaatct tctcttataa atattttgac taccttttac aaacctacat ctggaaatgt
     4081 aaaaatgttt ggaaaagatt tagtagataa tccttcttgg atacgtactc aaattgcttg
     4141 tgtagcacag caaatctcta ttgatgaaca tttatccctt atggaaaata tggtatttca
     4201 aagtaaaatg tataaagtag aaccacagat tgcaaaacaa agaatagact ctttaattga
     4261 taagtttgac ttatctagtt acttaaaata tcctacttca tcatattcag ggggtgtaaa
     4321 acgtagattg gacatagcta tgaatatggt atcatctcct aaaatcctat ttttagatga
     4381 accaactgtt ggtatggatg tcgattcaag aaaatctatg tgggatatgt tattaaaaat
     4441 tagagatgag tacggcacaa ctatatttct aactactcat tatcttgaag aagctgaaca
     4501 attgagtgat aatatttgta ttatgaagaa tggaaaggat ttagcacaag gaacaccatc
     4561 aagtttacgt agttacatta gacaaaatat actacgtata actttccata atactgaaga
     4621 tataaaaaaa tataaagatt caattaaaag tactggttta gtaaagttta tgagcgtacg
     4681 agaaaattca atttttataa gtgtaaacga tagtagaact gcttttactt taataaataa
     4741 atggctttta gaacatgata tagaatttga tgcaatagaa attgtagagc caagtctcga
     4801 agatgtattt ttagcactta caagttctaa aaaaagctta aaggaggaat gggaatgtta
     4861 aatatcttat ggcgtagtat gaagtggcgt tttaaaaatc caatctcatt tgtagttact
     4921 atattacaac cttttctttg gcttgtgctt tatagttcta ttgcaaacca aactatgaat
     4981 aatataaata tcaataatta tactgctttt atactaccag gtatcattgt actggttgtg
     5041 ttttcttctt gtagtagtgg tggtattata aactttataa tgaagaacag tggaagtttc
     5101 tatagagtct taattgcacc tatcagtagg tattcaatcg tccttggtca attattagaa
     5161 gctatcttag tttccttcat agaagtaact attttgtgta tagtaagtat attcttctca
     5221 gtaaggatag aatctggcat tggtggaatc ctacttatga tagtactgat atttatgaca
     5281 gccttctttt tatcaagtct agcttattct ataagtttat tattgccaaa tgaaatagtt
     5341 tatgaaacaa ttatgactgc aattgtactt cctattttct ttttaagttc agctcttttc
     5401 cctatagaaa gtttatctgg aggattaaaa gtagcagtga tgctcaatcc atttacccat
     5461 gttattaacg ctttacgtag cttaatattt ggtgagacta ttcttattgg agatatactg
     5521 cctgttatcc tactattttt aatattgtgc tgttcaagtt tttcacttgc tatgtggaga
     5581 ttaaaaaaag aaatggtaag ctaagtttta agcctattaa taacaaataa taaaaatgga
     5641 ctctattata tagaatccac ttttattatt tattttaata tttctaaaac cgaatttaac
     5701 acagcatgac cattacaagt accatagttt accatatcta ttatagatac tggaactcta
     5761 tctccaacta tagactttac gtcatcaagc ataaatctaa gttgtggtcc aagaagtaaa
     5821 atatcaaagt tatcaagctc cgattttata tctccttctc caactgccca tattttacag
     5881 tctatatttc ttttttttgc atccttctcc attttattta ccaatagggt agaagacatg
     5941 cctccaacac acactaacaa tatcttaatc atttatagct cctttttaac caaatctaca
     6001 tatatcttga ttaaaaagta tatgtagaaa agtttatcat ttataattgc ttaaatttta
     6061 ctcttagatt atatcaaatt tttccatttt tttcactacc agagccaatt ttatattttt
     6121 aatgtccact tttccacttc tttagagata tcttcagctt ttaatatagc tgatattata
     6181 gcatacccgt cactttctaa acatttaagt ttatctatat tgtctaaatt tatacctcca
     6241 attagcacaa atggcatgtc tactttttct ttaatttctt taacagtttc aaacgaagct
     6301 gattttgcat ctaatttagt agatgttgtg aatatagacc ctacacctaa atagtctgcg
     6361 ccatcatttt ttgcacaagt agcttcttct aaagttcttg ctgatactcc tagaatttta
     6421 ttgtttccta taagctttct aacctctctc acatcaatgt cactctgtcc tacatgtact
     6481 ccatctgcat cacaaataag tgctatatcc actctatcat ttattataaa cttaacacca
     6541 tatctttcag ttatttctct aagtttcata gcttttctta aaaactctct tccagaagca
     6601 tttttttctc taagttgtac agttgttatt ccagaagata ttgcatcttc aagacattta
     6661 taaaagtctc tacctttaag catttcagag tcagttacca aatacaactt taagcatttt
     6721 tttaaagatt ctttatcaat cattttaagt ctcccttact ctttattaat ttaaaaattc
     6781 aatattagaa tttttactta aagtatctat atccatttga taaatagcat tcataagatt
     6841 ttctttaaat gtacctattg gtggattatt taaattagct aattccccac ttaaagacat
     6901 tgtaagaaca cccattgttg ctgcttctaa tatattctca gtaacagcca taaaacttgc
     6961 aatcaaacta gcagtcatac atccagttcc tgtaattcct tttaatttat cagttccatt
     7021 gtttatttta tatatacctt taccattagt tattatatct atttttccag ttgctacaac
     7081 tacacattca agcttttcag caatctttct tattatctca gtagaatctt cttcttcatc
     7141 aaatgaatct acacctttac cccttacatt aaagccacct ataaacttta tttctgatac
     7201 attcccttta acaacactaa atttgacttc atttaaaagt ctacttgtaa gttctgctct
     7261 cgctttgctt gcaaatactc caactgggtc caaaacaaca ggtttattgt acttatttgc
     7321 tgcttttcca gctaatagaa ataaatccaa catatttgag ttcatagttc ctatatttat
     7381 tactacagaa tttgctacac ttaccatttc ttctacttcc tcatatgaaa aactcataag
     7441 aggacttgct cctacagcta aagtcacatt agcacaatca tttatagtta cattattagt
     7501 ataatgaatt accaatggat ttaatttttt tacatcttta attaaattat acatattcta
     7561 cctccaatat ttttttatta ctcaaattta taaaaatgat gtactggtcc tactccatga
     7621 cctatatcaa aacttctttt tattgcttct gttatataga ttttacttag atttactgct
     7681 tctgttattt catatcctaa tgctagataa gatgttattg cagaagataa tgtgcaccct
     7741 gttccatgtg tatttttttt atcaattctt tcacttttat aaactttaaa tatattttta
     7801 ccaactaaaa tatccactgc ttctccatct aaatgacctc ctttcataag tacaaattta
     7861 ggtccaagtt gtagtatttc ttctcctact ctcttcatat catctacatt atgtattttt
     7921 attcctgtta tttcttctgc ctctggtata tttggtgtaa ttatgtatgc aagtggtatc
     7981 aaatacttaa ttaagttttc ttttgcctct ggctttaata gataatatcc acttttagat
     8041 atcattactg ggtccacaac caaatatttt ggattgtatt tctttagatt ctcaactatt
     8101 tcaagtataa tctcaggact tgaaaccatt cctattttta cagctcttgg aggtatatct
     8161 tcaaagactg cctctatttg tttttttatt attttcttat ttaaatcctc tacagcaaaa
     8221 actcctttag tattctgtgc tgttatagct gttattacac tcataccata agtaccaatt
     8281 gcactaaacg ttttcaaatc agcctgaatc ccagctcctc cagatgaatc agagccagct
     8341 attgtaagtg ttggtatttt ataattactc attgttgcct cctaaagttt aatttaaata
     8401 acttaattaa aaaagctata cctacaatag gtatagctta gaaatagcat atatcacaaa
     8461 taatacatac ttgaattttt actaagcttt cctacgctgg tattatccag atcaggtgta
     8521 agggtcggta tacctctcag ctcttggctc ccctagctat tttaatttac tatatttata
     8581 acttcattat attacaatag tatttattgt caagttttta cactatatgc acccattata
     8641 atgttactat aaatatttat gttaattgtt ctatttcctt accaataata taatcttctt
     8701 tattatcaat taaatttata agtatacttg ggttttttat tatagacaat tttttatttc
     8761 tagttaattt ttttatatag tattctaact tcatagcatt gctttttgtg ttggtcacaa
     8821 aataaacttc tagtttttca aatccctttg ctcttgtgta ttttgaatta attcctaact
     8881 tatgttcatt cattcttcta actatattac tggtatatcc agtgtataga gaatcatctt
     8941 tacatcttat aatatatgta aaatacacct taaatcacat cctaacatta aatatataaa
     9001 tattatatta tagaattaaa aaaaatggtg tgtattttga atatgcaatc ttcaaagaat
     9061 taattataat atatgtataa aacatactct acatgaatct aatgctaaat tttgacataa
     9121 taataacata tttatttcta taataaaaaa ttcacaaatt ataaaaaata aaattttcaa
     9181 aaaaattata attttcaaaa aaacaaattg acacatataa aaaaattatg ataccctaat
     9241 aaaaaggggg caatgattat ggaaaacaat ttaaatatag ctaaaaaaaa taatactgaa
     9301 ttattacaaa atgactcact taaggtcaac ttacagatag gtgtagactt tacactttta
     9361 tcaaaatcac ttaaagcctt ttataaaaaa gaaaaagatt tttattgcat tgctcttgcg
     9421 ccatgcaatg tatctagtga aaaagcaaat agaaaaataa gtatttacga tatgataaaa
     9481 gaaattaata ttttaattga aagtataact gataaaaatg aacttttaag tgaagaactc
     9541 tttactaata atttatcaga attttatgat gaatctttag atgagattta tatagactta
     9601 aaacaagcat atttatatat taaaaagaat acagatagtc ttaacataga agactcaatt
     9661 gaatatgtat ttgatgtaga tataagcaat gatataaaag caaaggaaaa tccactaatt
     9721 aattttaatt cactttcttt agtagtatgg agtggcgaaa caaaacatat tttacaacaa
     9781 atgaatattt tagacttaga ggatattcac tcttattata tggaaaataa tacaaatata
     9841 actatctatg aaaataatac tcttcaatat aaatttgatg aagaatctga agatataaaa
     9901 gacagtagca tagatataat tgaacaaatg cttatacttc ctgatgactt gaattatgat
     9961 aaagatgaag ttgaaaatat gaaaagcaga cttgcaaaaa taaatattaa atatttacag
    10021 acacttaaag aaaaggatat aaaaataaaa ttaataaatt caaatctaac tgatgaacca
    10081 gaatttagtg atttaaaata tcaattacct ccatgttggg taagatcagg caagacttgg
    10141 aaagatgtac ctggtatata tagaaataat tctatagtag ctaaaatagg ttatagtaac
    10201 cctagctatg caaatgtaca ttcatcaaaa aatttagaat tacatgaaac tgcacatgct
    10261 atagataaaa atgttttaaa taaaaaatca aattctgaag aatttatgga agtatttgct
    10321 caggaaagat ataagcttta tgaccctaaa caagttgcac atgcatatat aagcaagttt
    10381 atagaagaat tttttgcaga atcatttgtg cattattatc ttgatgaaga ttcaaaaaat
    10441 actttaaagg aaaattgtcc tctaacatat gattttcttg aaaaacttga actaaactat
    10501 taagtgcgct aatatccaat aatacattta actatcctct tttcatcaac aataaccata
    10561 taataattta tactttgaat aaatcactat ctataaagga tttcttagta tttatgttat
    10621 aatatttata atggttatgt tttaaaatag tttatgtaac ccataattta aataaataat
    10681 aaaagaagga tgaaaagtta tgaagttaaa tagaaatgat agctgttggt gtggaagtac
    10741 taaaaaatat aaaaaatgtc atatggatat ggataataaa atagattcat ttgctaagtt
    10801 aggtcatatt gtaccaactc atgatattat aaaaaatgaa agtcaaattg aggaaataaa
    10861 aaaaagtgct gtaataaaca caaaaatttt ggattatatt tcagataaaa tcgaaattgg
    10921 aatgtctaca gaagatatga ataagttggt tcatgacttt acactacaaa atggagctat
    10981 ccctgctcca ctagactata atggatttcc taaaagtgta tgtacatcag taaataatga
    11041 agtttgtcat ggtattccaa gcaaggacat tatcttaaaa gatggagata ttataaatgt
    11101 agatgtttct actattttta atggatacta ttcagatgct tcaagaatgt ttatgatagg
    11161 taatgttaat gaaaatgcaa aaaaattagt tcaagttaca aaggaatgtg tccaaaaagg
    11221 tcttgaagtt gccaaaccat ggggattttt aggagatata ggtgatgcta tcaactctta
    11281 tgcaaagaaa aatggatact ctgttgttag agaaataggt ggtcatggag ttggattaag
    11341 ctttcatgaa gacccttttg taagttatgt atctaaaaaa gggactgaga tgctattggt
    11401 tcctgggatg atattcacaa tagaacctat ggtaaatgaa ggaactcacg aaatttttgt
    11461 tgatgaagaa aataattgga ctatttatac aaaagatggt aaattatctg ctcaatggga
    11521 atgtatggtt ttagttacag aaacaggggt tgaaatacta actgaataaa agacaaatgc
    11581 ctataaaaat tgaaaattca atttttatag gcatttattt tattaatgca catctattgc
    11641 atcaactgga cagctttctc tagcttctgc tgctgattct tcagcatcac ttggtacttc
    11701 atcaactata acatgtgatt tcccatcatc cttcatatca aatacttctg gacatattga
    11761 tggacataat ccacaaccta tacaagtatc ttgattaaca tttgctttca taataaaatc
    11821 ctcctagacg ctacaaattt aaaatcttgt ttatcaaaga ttgatgaacc aaaactgtaa
    11881 cattcagttc ttttttatta tgtcctcaaa tttgaaaaaa atacctttta aaataatttt
    11941 tataaagttt attttatagt tatataaatc ctatatataa tatacattat ctaattcatc
    12001 atactcatag gacacttctt ttttattctt ttttacaggt tctaggtttc ttatttttac
    12061 tatttttgct ggtataccaa caactgttgc accattaggt acatccttaa cgacaacaga
    12121 atttgcacca atttttgaat ttgaacctat atttaaaggt cctagtattt ttgttccagc
    12181 accaattaat acatcatcac caacagttgg atgtctcttt ccagtatctt ttcctgtagc
    12241 tcctaaagta gctccttgat atattgtcac cctattacct acttcagctg tctctccaat
    12301 tacaacaccc attccatggt ctatcaatat accttccccc attttagcac caggatgaat
    12361 ttctatgcca gtcataaatc tggatatctg ggatattaat cttgctgtaa aaagcttctt
    12421 cttcttataa agagcatgag ccatcctatg cattatcatt gcatgaactg atggataaag
    12481 taaaaaaact tctatcttag acctagctgc tggatcattc ttcattatat attcaatatc
    12541 tttatttatt ttcttaaaca aagtattcac tccctcaaat aaaattatta aaatattccc
    12601 atagacatat acttatcaac tccatctgga gctactgtaa ctatcttttt atctggatac
    12661 ttttttgcca tctctatagc accaaatata tttgctccag aagatatccc tactaaaatt
    12721 ccctcttttg ctgcaagtaa tttaactgta tcaaatgcat cttcatcatc tactaatatt
    12781 acttcatcaa ctacactaga atcataattt tcaggaacaa agttagcacc tattccttgt
    12841 attttatgag gtccagtctt gttttctgat atagctggag atttttttgg ttcaagtgca
    12901 attactctta tgtttggatt ttgttctttt aaatatcttg cagttcctat taaagtacca
    12961 cctgttccaa ctccacatac gaatatatct aaatctttga catcattcat aatttcaact
    13021 gctgttgttt tataatgtgc atttggatta tctttatttt caaattgttt taagctttta
    13081 tagtttggat tttcttttaa aagttcattt gctttttcta tggaacctgc cattccagct
    13141 ttaccatctg taagcaccaa atttgctcca taagccttca ttaaatctct tctctcaacg
    13201 ctcattgttt cgggcattac gattactact tcatatcctt ttaattttcc tatcatagat
    13261 aatgcaatac cagtatttcc actagttgct tcaactagta catctccctt ttttaattct
    13321 cctctttgct ctaagccctc taacatttca taaacagctc tatcttttat acttcctgct
    13381 ggatttactt tttctaattt tacatatatg ttagaacacc cattagtaca gcctaaatta
    13441 ttcagcctta ctacaggtgt gtttcctatc aactctaatg cgttattata taacatacta
    13501 tttcctccta tatgtccaaa tctattatca aattgataac gataaatata ttatagtatt
    13561 atcattttga taatgcaacc tttttttgga ttttatactt acaatcactt tattccaaat
    13621 attagtaata ttcaaataaa aaagtgaaaa tcattattga ttttcactct ttaaaattta
    13681 acctttaaat taaatcttga gtaatatttt gttactaaaa ttccacaaaa tactatataa
    13741 gacctaatat ttttgcgacc tctatattaa cctttagtac attaactcta tcttcaccta
    13801 aaactccaaa aagttttttg gaagtattat catctacaat tttttgtaat ttactttctg
    13861 atattccaga cgcttttgct atagctggta tctgaatttt tgcagaggct ggacttatat
    13921 tagggtcaag tcctgaacct gatgctgtaa gtaaatctgt tggtatatcc tctcttttga
    13981 cagttggatt atcctttaaa aacttttcta tatctttttg tactctatca tgaagttctg
    14041 gatttgtagc tccataatta aaagaccctg atgatacacc tccataagat gcatttccat
    14101 ctttatctgg tactaaatct tcttttgtat aagtattata gtttactgat gatactcttc
    14161 ccttaaagaa tctcgcatct gtaaagcttt gaccaataag ttctgaacct acttttacac
    14221 catttacttc tatcatactt ccattggctt tatctttaaa tgcaacttgg cttacacctg
    14281 tcaaaacaag tggatataca agtccacaaa caactaacaa tacaatactt acaagtaatg
    14341 ctggctttaa tgttttcatt tttatatcct ccatttatta acctaaatta agaatcatta
    14401 ctaaaggtgt aatcaataaa tcaattaatt ttattcctaa aaatggtact ataactccac
    14461 caagaccaaa tataagcata tttctcatta acatagcttg tgatttcata ggtctatatt
    14521 ttactccctt cattgcaaga ggtattaaac atgggataat tatagcatta aatattaaag
    14581 ctgataatat cgcactaaat gatgtagaaa gtttcattac atttaatatc tccatttgag
    14641 gtattgcaag agtaaatata gccggtataa tcgcaaaata ttttgcaaca tcatttgcta
    14701 tactaaatgt tgtcaaagcc ccacgagtta ttaaaagctg ttttcctatt tcaacaactt
    14761 ccaatatctt agttgggtct gaatctaaat ccaccatatt tgctgcttct tttgcagatg
    14821 ttgtaccact attcattgca agacctacat ctgcctgagc aagtgcagga gcatcatttg
    14881 ttccatcacc tgtcattgca actattttac cctctaattg ttcttttttt atagcatcta
    14941 ttttatcttc tggtttacat tctgcaataa atccatctac tccagcttct cttgcaattg
    15001 tagctgctgt caagggatta tcccctgtac acattattgt ctttattcct atctctcgaa
    15061 gtctagcaaa tctttctaca agccctgatt ttactgtatc ttttaagtag ataattccat
    15121 atatctcatt atctacacac actactaaag gtgttcctcc tagtttagct actcgattta
    15181 cattttcttc taaatcttct ggcactgtac caccagattc ttttactctt tttattatgc
    15241 tatcataagc accttttctt atagctctac catcagctaa gttaatacca ctcattctag
    15301 tttgtgcaga aaattctaca aactcagctt cctctgcctc ttttgtctta cctgttgaac
    15361 ctaatttcat acctaaatca actatagatt ttccttctgg tgtatcatct tttaatgaac
    15421 acataacaga ataatttatt aaatcttttg aatctgcatt tccaactgct ataaattctg
    15481 atgcaagtct attcccataa gtaatcgtac ctgtcttatc caaaatcata gtatcaacgt
    15541 ctccacaagc ttctactgct tttcctgaca ttgcaattac attaaatcta gttactctat
    15601 ccatacctgc aataccaata gctgacaaca atccacctat agtagttggt attaaacata
    15661 ccaaaagagc tatcatagta gaaattggaa tctttacacc tgcatagttt gccattggat
    15721 aaagagatat taaaacaacc ataaatataa gtgttaaccc tactaacaaa gtatttaaag
    15781 caatctcatt tggcgttttt tgtcttgaag caccttctac aagagctatc attttatcta
    15841 agaacgattc tcctggtttt gttgttattc ttatctttaa ccagtcactt acaactgtag
    15901 taccacctgt aacagatgcg aaatctccac ctgattctct catgacaggt gcagattctc
    15961 cagttattgc tgactcatcc acagaagcta tgccctctat aacttctcca tcatttggga
    16021 taacttctcc aacatttact aaaacaatat caccaagttt tagctcactt gcacttatca
    16081 ttttttcatt tcctaacgta tctataaccc ttgctactgt atcttttcta gttttcttaa
    16141 gtgtatctgc ttgagcttta cctcttccct cagctacagc ttcagcaaaa tttgcaaaaa
    16201 ggactgttat aaataagatt attgttacaa tcccattata agctcttaaa ttattgcctt
    16261 catcaccaaa caaactaggt actattgtaa gtatcaatgt tactataaat ccaatttcta
    16321 caacaaacat aactggattt ttcatcatat atttaggatt taatttttta aaagcttcta
    16381 taatggaagt tttgaaaata tcttttgtaa taaactttgt ttttttagtt tccataaaac
    16441 tttactccct cccaattttt tagtgccaca acactagatg ctccgaaata ggtccaagcg
    16501 ctaaggctgg gaaaaatgtt aaagcaccaa caattaaaac aacaagtact aaaactattg
    16561 taaatgtaaa attatccgtt ctaagtgttc ctatactttc atttacagct tttttagatg
    16621 ctaacaaact tgatattgct agtagtacta taatagacac atatcttcca aagaacataa
    16681 ctatacctgt tgttatattc caaaacatag tgttatctcc tagaccttca aatcctgaac
    16741 cattattggc tgctgaagat gcaaactcat acaatacctg tgataatcca tggaatccag
    16801 gatttgaaat tccttcaagc cctgctggta ttgctactga aagaccagag aacattagta
    16861 ttaaaagtgg atgtaatata ataagtaaag caataagctt catttctttt ccttcgattt
    16921 tcttagttaa aaactctggt gttcttccta ccattaatcc acataagaat actgctatta
    16981 tagcgtacat caacatattc atcagaccta cgcctttacc accaaatact acatttagca
    17041 tcatatttaa aagtggtact aatcctccta aaggagtcag tgtatcatgc atattattaa
    17101 ctgtacctgt cgtaaaggaa gtagtcactg ttgtgaataa tgatgattgt gctataccaa
    17161 atctaacttc cttaccttcc atacttccca tactttgatt aagccctaat tgtgataaaa
    17221 tagggtttcc tgcactttct gctttaaagc acactactag acctattaaa aatatgattg
    17281 acattgcgcc aaacacaacc caaccttgtt ttttattctt tatcatgtga ccgaatgcaa
    17341 caacacaagc tcctggtaat atcatcatag ataatatttc tactatattt gatattattg
    17401 ttggattttc aaatgggtgt gatgaatttg aaccaaagaa tccaccacca tttgttccta
    17461 aatgttttat tgattcaaga gctgccacag gacctctagc tatatcttga agctttcctt
    17521 ctatagtagt cactgtttct gtcccagcaa aagtttgtgg tactccttgt gtaactaata
    17581 taagcccaat aattaaagct cctggtaaaa gtactcttgt tgttatccta attaaatcta
    17641 cataaaaatt tcccaatgtt ttctttttac cgactagacc tcttacaaat gccatagcag
    17701 ccgcatatcc tgttgctgct gatgtaaaca tcatatatat tatcactgtc atctgactaa
    17761 agtatgaaag tcctgattca cctgaataat gttgaagatt tgtatttgtc ataaaactta
    17821 ttattgtatt aaatgatagt ccttgttcca tacttttaat tccacttgga ttaaaaatat
    17881 gcatactttg agttctaagt atgatatatc ctataaatac cataactgca tttgtaaata
    17941 ataaagctaa tgcatactgt ttccagttca tttctttttt cttatctata ctacatacct
    18001 tatatataaa attgtccact ttatcaaata gtctatctcc aaatgttttt tgattagtag
    18061 ctatatgata taaatacttg cccataggta ttactaaaat cataaaaatc gctaacacta
    18121 ttactatctg caccataaaa attgttcccc ccaattcaac ttacttatta gccttatact
    18181 gtttaaaatt tttcaggatt aataagcgca tataccaaat atattattaa cattataatt
    18241 acaactgcca gaaaccacat aggtaatccc tccatacatt acattatttt ttctcagttt
    18301 gtttcccaca ccaatcagca agtaacttca tggaaacaaa tcctatgaat aacaatgcta
    18361 tcataactat atccaacatt ttagctcctc catttaattt aatctctttt acaaatttta
    18421 gttttgtatt gctacttcat ttatcttatc attttaggta taaagatagg attaagaaac
    18481 atccattaat gattagtatt tcataaatat aaaattgggt ttttaaaatg cacatctagc
    18541 aatcgtattc ccagtcattc caacataatt ctatatttga gatttgcaaa aacctagatg
    18601 ggattatact ttctataaag atttagacac aaaaaaggtg gcaaatctgc cacccctaat
    18661 aaaatctaaa gttatttaat ttatattaac attctggaac tatatcatta cttatatcca
    18721 tatccaaact tttaccatga taataaataa aatcaattac cttgccacta attgtaacag
    18781 caatcaaaga ataaaagata tcaaacgctg atagataaaa taatgataac aacaatacaa
    18841 taacatctgt tgctaaataa acttgcccta tgttcaaaga tgtagtctta gaaattacta
    18901 atgctaaagc atcgtctcct cctgatgcac aacctgcttt tactactata cctacaccag
    18961 ttccaacagt tacacctgcc aaaatagttg ataaaagcat attttgtgat tgtggtacta
    19021 gaggtcctat agcttcaaat aagtcatata acactgaaaa agttatagaa gctactattg
    19081 aatatataag gaatttttta ccgaaaaatt tatatcctac taaaagtagt aaaccatcta
    19141 ttacaacatt tgctattgct ggttgtatat tgaataaatt ttttaacagt agtaaaatac
    19201 ctaacactcc accttctgtt atgttgttta aataataaaa attataaact ccaaatgcta
    19261 atattgctga acctaaaaat ataagtccaa tcctttctaa aaataaccgc ctggccatac
    19321 atatcccttc ctttttctga atttatatat attatataca aaagaattgg ctttgtatac
    19381 cgaacttttc aaatctttaa ttatacaaaa tttctatatt tttttcaaca atttttttat
    19441 gtttttttat gtttttttaa taccacacct acactatagt aattccaata ctttagacgt
    19501 tttccttttt tccataagtt tatattagtt ttacttataa tttagtataa ttaagtcttt
    19561 gtttatatta attttttatt tgtttagaaa tactattcaa taatttatca ctaaattaaa
    19621 ttaaaaaaag agttacttta tatagtttct aaagtaactc taaaaatctt atttattatc
    19681 tatgacaatt taccattcac ccttgaactc tgaaatatta tctttgttaa cttcaacttg
    19741 tttaatttgt gtaacaggtt ctattttttc acctttaagg aatttagatg ccttttctat
    19801 gactaatcca ccttcttcta cagcagacat ataagttgta ccatctatta acccatcagt
    19861 tatagctttt aatccatctt tacttccacc caaacctatg actttaactt tatccttaag
    19921 accagcatct tttaaagctt gacctgcacc tgtagcagaa ttatcatctt gacatattat
    19981 aacattaaaa tcagttttct tttgaattaa atcttctgtt acttttaaag cttgctcttt
    20041 ctcccaattt gaagtttgat tataaactat ttctatatta ctatctttta attctttttc
    20101 aagacctttt tttcttaata cttgtggagt agttcctggt actccttcta ttaatacagc
    20161 tttcccacca gtttctccta ataagttttt agcaattttt ccagttagtg ctcctgtttc
    20221 ctcctcattt tgtcctatat atgtaactaa acctttaaat tctccagttg ggttacttcc
    20281 tatagaattt gtaaatgcta ctatagggac gtctgaatca ttagcagctt taactattct
    20341 ctcacttaca gcagaatcaa ctccacataa tgcaatcatg tctacccctt tagccataaa
    20401 atcttcagct tgtgataatt gtttactagc atcccattga gcatctgcat attccacgct
    20461 tacacctaat tctttagatt ttgcatctat accttgttta gttgctacaa agtaacctgt
    20521 tgaacctggt agtaatactg ctattttctt ttctccattt ttttctttgt caccaccatt
    20581 tgaacaacca actaaaccaa atatcataac aagtgataac aacaatgcta aatgtttcat
    20641 taattttttc ataaaaaaat cccccttatt ttttatcttg aagcaagttt tttcttgcta
    20701 taagaatcaa atccaactgc tgctaaaatt attaatcctt taaatatgta ctggaaatat
    20761 acatctattc caagaagatt aaaactgttg tttataacac tcataagtaa tactcctata
    20821 acagttttaa gtacagaacc agaacctcct gccactgatg tacctcctat tactacagat
    20881 gcaattgcat ccatatcata tccttcacct gcaatagcag ttgcagtccc aagtcttcct
    20941 gttagtacga ttccagctat tcctgcaagt acagcattaa taataaacac tttcactttg
    21001 tgagaatcta catttatacc attaagttta cttgcttctt gattccctcc tacagaataa
    21061 acatatctac caaacttagt tttattaagt acaataaatg aaactaacac cactataaag
    21121 aaaattacta ctggtattgg tatattaaac aaatatccat ttccaatgta atcaagtatt
    21181 ggtatatttt cagtaattgg atatcctcct gtaagcatca acagcaatcc tcttacaata
    21241 gtgaccatag ccatagttgc tattaatgat ggtacattta tctttgcaac tactatacca
    21301 ttgattaatc ctattactgc acaaatcgct acacctaaga gcatggatgc aaacaaatta
    21361 gttccaccag tcgcaaattt cattgttata gcacctgcaa gagctgctac tgctccaact
    21421 gatatatcaa agtttccaga tattataaca aaagtcatac ctgctgaaat tattccaatt
    21481 atagaagatt gtcgtaaaag attaagtata ttttgtttac ctaaaaatga tggagttgca
    21541 atacttatag ctatacataa agctaaaaaa cctaataata ctccataatc tcttaaattt
    21601 aacttctcaa aagctttctt catctcagtc ctccattttc attttttata cattaaatat
    21661 actgttcatt atatcttgaa cctgtatatt ctcatttttt aaatcatcta caatagaacc
    21721 ttctctagtt actaaaattc tatcacttac tcttattact tcttctaaat cagaagatat
    21781 aaatattact cctattcctt cacttgaaaa ttctttgatt gtattgaaaa tatcttcttt
    21841 tgcagcaata tctattccct ttgtcggctc atcatatatt attaaatcta aatctttctc
    21901 taaccactta gaaactacta ctttttgttg atttccacca ctaagctctt ttacttcttg
    21961 ctttacatca ctaactttta ttcctaattc cttaacacct tttttagaaa actcaatttc
    22021 tttatttcta tctataaaca aaaatttctt gaatttatca atttgtactg atgatgagtt
    22081 tgtgtatata gcttgttcta aaattaatcc tagatttttt ctatcttcag gaatcaatcc
    22141 tattttgttt tttatagcaa ccttaggaga agttatgtct acttttttac cattgataaa
    22201 aatgtctcca ctatctttat tatctatacc aaatatcaaa tttataattt cagttcttct
    22261 tgaacctaca agaccagcaa gacctacaac ttcgcctcta taaactttaa atgaaacatt
    22321 ttttattttg ttagaccagc ttatattctt tacttctagt actggtggct cttcatactt
    22381 tgcaaatgta taatccttct tctcaataag tttgtgatta tcatttcctg tcataagttg
    22441 agttatcttt aatttagtta aatcctctac atcataagtt ccaatcataa ttccattttt
    22501 cattatactt gcactatcac atactttaaa aacttcctct aatatgtgag aaatatagac
    22561 tattgtttta cctttagact ttagtctact tattatttta aatagcccat ctttttcatt
    22621 attagtaagt gatgttgtag gttcatccat tatgataatt tcagaattac atgaaacagc
    22681 tttcattatc tcaaccattt gttggtttgc tacacttaaa ttagaaacta ctgtatgtgg
    22741 gtctatatta aaatcaaaat cctcacaaat tttcatatat tcctcaagca tgtctttctt
    22801 gtttaaaaac acacttcctt ttaaaatttc tctaccaaga aaaatatttt caagtactgt
    22861 aagcgttggt atcaagctta attcttgata aattatacta ataccatttt ctttagaatg
    22921 atgtgaattt ttaaacatta tttcattacc agtcaaaatt actttacctt tagtttgaga
    22981 atgaactcct cctaaaattt taataagtgt agattttcct gctccattag ctcccaaaag
    23041 agctcttact tctccctttt ctaactcaaa atttatccct tttaaaacat ctacctttcc
    23101 aaaactttta tatatttgtt gcatttctaa tatcttactc actatatcac ctcactaatt
    23161 ccgaaaccat tttataattt catcaatatt ttcttttggt gctgataaca aatcccaaga
    23221 taaaacatat ccttttactg ttgtcttatt tatatttgaa atattttcac ttatgtactt
    23281 aggtgtaaca ggagctataa tttccttttt atttatttca actcctacat atatatcttt
    23341 attaagattc cttgtcataa aatcgatttc ctctagtaca aattctatag gatatacatc
    23401 tccttcatac tctttaattc ctaaaatatc taagaaattt ctttttccaa tctctttatt
    23461 cttatgtatg gattcatcaa taaacctttc aaattcaaat ggtaaaccag ctggagcttt
    23521 tgtagttcta tacatcattg gttttataaa atctgctact tcttgtaatt tttttaaatc
    23581 ttgacctgta aagtaagaaa taaaaggaga aaaagtatct atacctattt tcaatccctt
    23641 acttttaaaa taactactta tctctaataa tgacttgtat atattttgat tttttgtttc
    23701 aaaaaaatct tcccatattt tatttttaaa tttgtatttt ccattaatgt attctgttat
    23761 accagatgga gtatcttcat tatctataac tcccttagac acattattca tttctctcat
    23821 taacgacttt ttatcaatat tatttttttc atatatattt aaacaatctt tacaaaaaca
    23881 attaaaaaca ccatttagtt tattcgaaaa agaaccatac cttattttat ctaaaaaaat
    23941 tccatcaaat ttaatatcac taaaataagt actatatatt ttctttatat tttttatatt
    24001 ttccttatta tttgggcaat aaaattcaaa attttcatcc tcttgtaaag catagccctt
    24061 gacttccttc cccatataat caattagtaa attacttttt tcaagtaatc ctatttcaga
    24121 aaaacatggc aaccaaagat atagttcaac attatattta tttaatagtt ttttagtttc
    24181 tacatataat tcttcatcta cactccatcc catgataacc ttttctatat caatacagtc
    24241 caataattct agtagtttac attttatatc attaaacttt atttctttag ccttaaaacc
    24301 tccagtaaaa atttgtacta ttaccttttt tctatccatg cttaactctc ctattagcta
    24361 atgccatagc aagtcttatc cccccatata ctggctgatt ttcttgagat ataaatttta
    24421 aatttttatc tgtattttct gtttttaaat tattcattaa catttgagca tatacagttt
    24481 tatttaatgt accaccagac aaaactatat ttatttcgct tttatctatt ttcattggat
    24541 tagatatttt tctcaatatt acttgagcta atttagatag ttcagttgat gattctctta
    24601 atatttcaat agctaattta tcaccttgtt cggcacaatc aatcactaat gaagctaggt
    24661 ttgctatttc aacattattg tttatatcag taatcatata tgcaagttgt tcaaaactat
    24721 ttgctccaaa gaaatccaag acacaactaa ataaatcact atactcatga cattcatcac
    24781 aataaagcaa tactttcttt aataattttc tccctatatc atatccagaa cccaaatcac
    24841 taaaattata cccccatcca ccaactctat atatttctcc atcttcttta attcccagta
    24901 taattgaacc tgtcccagcc acaattacta aaccagggga atcagcttgt gcataaaaag
    24961 ctaaaactgc atcatttgcc agatatattt tttctttgtt aatcccaatt tttaatattt
    25021 catccattat aatcttataa tcattaggag aatcacaacc agatatacca aatacagtgt
    25081 actcaatatc gtcataatcg atatataaat cttttaatat ttttttaagt ccattattaa
    25141 tattttcata tgttttttta attccaactg acttataatt agttgaacca gtggtatgtt
    25201 ctttaagtat gtttccatct aagtcactta cacaaaattt agtttttgtt ccccctccat
    25261 ctattgaaac caaatatttc acattaatat cctccttttt tattagataa ggaatattga
    25321 tagtcagttt cttttaaaca catactaatt actccattta tacttctttc tctcaatgac
    25381 tctactttta ttaatttagg aaaattttct tctccaacat tattacttac atctttttct
    25441 ataaactcca tgtcactctt ggttaatata ctacactgta taatcatagc ttgtggattt
    25501 aggacacata caagagtcct tataaagttt gatattatag ataaaatttc tttcttaaat
    25561 ttattatctt tactttctaa atatcttttt cttaaactca taatataatc ttcaagattt
    25621 aagttgttag agttatactt accaaattct atatttagat aacttaattc tccagcaaaa
    25681 tttgtagaac ctaaatacaa ttcaccattt ataactatac ctgaaccaat accatcttct
    25741 aaatacataa gagccatatt tttatatttg tctttatact gtttttgata aatcccaata
    25801 gtagtaatat ttgtatcatt ttctataaac actttagtat tgtacttact ctttattaaa
    25861 ttttctaaat ttataccttc ccaacttttt atagttggta tatttttaac tacacctttt
    25921 tttacaactc ctgggacacc aattccaata gcccttatat tgtttccttt ttcctttttt
    25981 attagagtat ctataacaaa aaaagtatct tggttaacgt tttcatatgt atccattctt
    26041 atattaaatt cttcttgata aattatttca cctaataaat tggcaactac ccctatacaa
    26101 caatttctat aataatataa agctattatg tgttctaagt ttgcatttat ttcaaataat
    26161 tgagctcttc ttcctccagt agattcactt agtccactta ctttaacttc atttttcaaa
    26221 gctaattgct ccacaatctt attaactgta gctaaactca attcagtaat gcttgaaatt
    26281 tctggttttg ttataggacc ttcatttttt ataatgtttt ttattaaatc ttcatttact
    26341 ttttttatcg tttgcggttt tccaactata tttttcatag acaacacctt actttataaa
    26401 ctttatttat taagtttagt ataatattta caagttaaat ttgtcaataa attttcaata
    26461 aaactttaaa atcaaatttc tcaattgtct gcttcataat tattcttcct gactaagttt
    26521 tattgttcat attcctttca actgttcata ttag
//
LOCUS       DAGQWR010000049        26015 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.049, whole genome shotgun sequence.
ACCESSION   DAGQWR010000049 DAGQWR010000000
VERSION     DAGQWR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.049"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            145..1212
                     /locus_tag="MIW95_001752"
     CDS             145..1212
                     /locus_tag="MIW95_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBY3151081.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGDVLIEMEHPQQIIPEVE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            1309..1617
                     /locus_tag="MIW95_001753"
     CDS             1309..1617
                     /locus_tag="MIW95_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBY3151082.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            1610..3040
                     /locus_tag="MIW95_001754"
     CDS             1610..3040
                     /locus_tag="MIW95_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBY3151083.1"
                     /translation="MNNSTSIKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGAKIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            3118..3492
                     /locus_tag="MIW95_001755"
     CDS             3118..3492
                     /locus_tag="MIW95_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBY3151084.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWNLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            3486..5690
                     /locus_tag="MIW95_001756"
     CDS             3486..5690
                     /locus_tag="MIW95_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBY3151085.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLRIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            5690..7099
                     /locus_tag="MIW95_001757"
     CDS             5690..7099
                     /locus_tag="MIW95_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBY3151086.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELYIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            7084..8148
                     /locus_tag="MIW95_001758"
     CDS             7084..8148
                     /locus_tag="MIW95_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBY3151087.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERSIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKKLQDINLPKILIRIPMLSELEEVINYCDISFNSELETI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            8178..9599
                     /gene="nhaC"
                     /locus_tag="MIW95_001759"
     CDS             8178..9599
                     /gene="nhaC"
                     /locus_tag="MIW95_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBY3151088.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            10028..10492
                     /locus_tag="MIW95_001760"
     CDS             10028..10492
                     /locus_tag="MIW95_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBY3151089.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            10790..11152
                     /locus_tag="MIW95_001761"
     CDS             10790..11152
                     /locus_tag="MIW95_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY3151090.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            11747..12607
                     /locus_tag="MIW95_001762"
     CDS             11747..12607
                     /locus_tag="MIW95_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3151091.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIIANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            12865..14979
                     /locus_tag="MIW95_001763"
     CDS             12865..14979
                     /locus_tag="MIW95_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY3151092.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEHDVELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYVGTLTQLISM"
     gene            complement(15241..17484)
                     /locus_tag="MIW95_001764"
     CDS             complement(15241..17484)
                     /locus_tag="MIW95_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBY3151093.1"
                     /translation="MSNIIIKGLTQNNLKNISFKIPKNKIVVFTGVSGSGKSSIVFDT
                     IAAESQRQMNETYSSFIRNRLPKYRKPQVELIDNLTASVIVDQTPLGGNARSTVGTIS
                     DLYSSLRLLFSRIGEPYIGTTSYFSFNDPNGMCKNCSGIGRVKVINIESIINPENSWN
                     TGCINDSLFKPNSWYWKQYAESGLFDLDKPIKDYSFEEYNLLLYGSKNKDGKQENPKI
                     EGIFNKYNQTYLNRDISNMSKHTKEKSERLITDKECPVCHGKRLNKETLNCNILGYSI
                     ADMCNMELTDLYDLLLQIKDKRTETVIKTLTDGLKRMIDIGLPYLHLNRESSSLSGGE
                     AQRLKLVRYMGSSLTGMTYIFDEPSAGMHPRDVHRMNQLLKKLRDRGNTVLVVEHDKD
                     VISIADEVIDIGPSAGTQGGHVVFQGSYDKLLLANTLTSNAMNLSLPIKTDSRHPKGT
                     LPIRDACINNLKHIDVDLPVGIMTVVTGVAGSGKSTLISRVFAQKYEKDVIKIDQSPV
                     TATNRSMPATFLGFFDEIRKVFAKENNVEEGLFSFNSKGACPVCDGKGVLVTELVFMD
                     PIINTCEACKGTRYSEEVLSKLYKGKCIVDVLSMTVDESLEFFTEKKLCKILQTMKDV
                     GLPYMTLGQPLSTLSGGERQRIKLAKNLNKKGTIYILDEPTTGLHGSDIEKLMLIFEH
                     IVDKGNTVIIIEHNLDVLKQADWVIDIGPDGGKNGGQVVFEGTPLEMFERGCTITSDF
                     LRKSINI"
     gene            17816..18532
                     /locus_tag="MIW95_001765"
     CDS             17816..18532
                     /locus_tag="MIW95_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151094.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSESVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            18560..19498
                     /gene="blaCDD"
                     /locus_tag="MIW95_001766"
     CDS             18560..19498
                     /gene="blaCDD"
                     /locus_tag="MIW95_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBY3151095.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            19842..20525
                     /locus_tag="MIW95_001767"
     CDS             19842..20525
                     /locus_tag="MIW95_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151096.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            20522..23182
                     /locus_tag="MIW95_001768"
     CDS             20522..23182
                     /locus_tag="MIW95_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151097.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(23363..24544)
                     /locus_tag="MIW95_001769"
     CDS             complement(23363..24544)
                     /locus_tag="MIW95_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3151098.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(24544..25206)
                     /locus_tag="MIW95_001770"
     CDS             complement(24544..25206)
                     /locus_tag="MIW95_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3151099.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVLLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(25351..25893)
                     /locus_tag="MIW95_001771"
                     /pseudo
     CDS             complement(25351..25893)
                     /locus_tag="MIW95_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT         9850 a         3157 c         4516 g         8492 t
ORIGIN      
        1 atattatttt ttatttatga aaatacataa taaaataatt atagtttcaa cgttccaata
       61 aatatttact ttaattttaa agttggcacg atttatgctt taactaatta ttgagaatat
      121 ataaattttt aggagggtgt atatatgaga aaagtaagag taggaatatg gggatttgga
      181 gctatgggaa taggtatggc taatatgatt ctaaagaaag aaggtataga aatagtatct
      241 gtttgtagta gaagtacttc agggaaaagt atgtatgatg ttttaggaat tgaaagagga
      301 gaaagaccag aagttattat caataaaaat tatgaagaag tttttagaga aaaaagtgta
      361 gatgttgttt tgttagcaac tgattcattt actaaaaagg cttttgataa aataattttc
      421 ttattaaata gaaaaataaa tgttatatca acagcagaac aaatggcata tccacaagct
      481 gatgatgcag atttagctaa aaaaatggat gaagttgcaa aggaaaatgg agtaagtata
      541 cttggaacag gaataaatcc aggatttgta ttagatttac tagtattggc actttcaggt
      601 acttgtgaag aagttactag tataaaagct aagagagtaa atgatttatc tccatttgga
      661 aaatctgtta tggaggaaca aggtgttgga gtaacaaggg aagaatttat aaaaggtgta
      721 gaagataaga ctatagcagg tcatgtaggt tttgtagaat caataaatat gatagctgat
      781 ggattaggtt ggaaattaga taagatagaa caaacaaaag agccaataat gactacagta
      841 gatagaaaat ctaaatacgg agaggcattg gctggaaatg ttgctggttg tagacaatgt
      901 ggatatggat atgtaaatgg agacgtttta attgaaatgg agcatccaca acagataata
      961 ccagaagtag aaggtattaa aacaggagac tatgtatcaa taaaaggtat accaaacatt
     1021 gatttacaaa taaatcctga aatacctgga ggagtaggta cttatgcaat gatagtaaac
     1081 tctattccat taataataaa tgctagacca ggtcttaaaa ctatgttgga tataccagtg
     1141 cctagagcaa taatgggaga cataagaaat cagatagaag ttgaattgga agaagaatca
     1201 aaggctaatt agtaagataa cagttagcta attggtaaaa taatgtttaa tttaaaataa
     1261 cagtaaatta attagtaagg aatagtcaat taataagggg atgaaaaaat ggatgctaaa
     1321 attaatgatt gggttattat acacaatata gtattgactc cagaagaaag agctccacaa
     1381 gttccagagg atactaagaa agtatcctta gaaatgtggg ttaaaggttt tatacaaagt
     1441 gatgcttcta taggcgactt agtagaagtt aaaacaatta caggtagact tgtaaaaggt
     1501 aatttactta aagtaaatcc atattatact catgattatg gtaaatgtat accagagtta
     1561 ctccaaatag gaatccaagc taaagaaata ttatttggag gtgtatataa tgaataattc
     1621 aacatcaata aaagatatga gttatcaagc tgttatggga agaaataacg aaataatgaa
     1681 aaatgcaatc ggattggatt attcttcttt tgaacaagag ggaatagggt ttgattatga
     1741 gaaaatgatg agtgaaacag gatatacatt acaagatatt gaagcaattc aatcacagta
     1801 tgctgttgga aatactcctc taatagagct taaaaatttg actaaattag ctagaaaatg
     1861 tgctaaagaa ggtaaaggag ctaaaatatt tgtaaaagat gaagcaatga atgcatctgg
     1921 tagttttaaa gcaagaagag cagctactgc agtatatcat gccaaacaaa tgggatataa
     1981 aggtgtaata gcagctacca gtggtaacta tggtgcagct gtagcatcac aagcggcaat
     2041 gcagggatta aaatgtataa tagttcaaga atgttatgat tctaatggtg taggtcaacc
     2101 tgaaattata gaaaaagcac gaaaatgtga ggcatatgga gcagaagttg ttcagttaac
     2161 tgttggacca gagttatttt atacgttctt agttttatta gaagaaacag gctattttaa
     2221 cgcatcatta tattcaccat ttggaattgc aggtgtagaa actttaggtt atgagttagc
     2281 tatccaattt agagaaaagt ataaaaaaga ccctgatata gttgtttgta caaatgcagg
     2341 tggtggtaat cttactggaa ctgcaagagg attgataaaa gctggttcca ttaatactaa
     2401 agtagtagga gcaagtgtag atttaaaagg attacacatg gctagtgata atcaatttaa
     2461 taaaaaatca tttacaacag gacatacagg ttttgggata ccatactgta catggcctga
     2521 taggtcagat gtacctaggt ctgcagctag accacttaga tatatggata gatatgtatt
     2581 agtaaaacaa ggagaagttt tttatacaac tgaattattg gcacagctag aaggtataga
     2641 aagaggtcca gcaggaaata catctttagc agcagcattc tctttagctc aagagttaga
     2701 tgaggataaa gttatagtgg tacaagaaac agaatataca ggcgcaggta aacatatatg
     2761 tccacagctt acttttgcaa gagaaaatgg aatagatata aaatttggaa atccaagaga
     2821 agaaattgca ggagtaaact taatattacc agaaagacca gaattattga aatgtgttga
     2881 tattgatatg aataaaataa gaaagtcatt tataaaaaat tgcatatcta ataatcacat
     2941 tgataatatt gataagctaa gtaatcgaga tatagaattt ttaatggaag aagtaaaaag
     3001 ttcaagagat tttgttgttg atgtattaaa taatttatag taattattgt ttttttattt
     3061 aatataaaat aaaaattcgg aaaattctat taaatcaatg cgaatggagg aatgttcatg
     3121 aaaaaaagag aggatgattt tgaagttaga agaaaacatc ttcaagaact gagtgatgat
     3181 gagttaaaag aaaggttttg gaatttagct acccagatag tagaaccaat gatagaactt
     3241 ggtaaaaaga atacaacacc atcaatagaa aggtctgtct tgttgagaat gggattttct
     3301 tctcttgaag taaaacctat tttagaaggg gttatggaaa gaggtcttat aggtaaagga
     3361 gcagggcatg tagtttataa attggctaaa tctaaaaata taacggttag agaagcaggt
     3421 ttgttattag ttaagggtga atattgggat gaagttacat gtttatttag agaaggggta
     3481 gaatcatgtt aaaggaaaat aaaaaattgg atatagacta tatattaaaa gatttggata
     3541 aatataaacc aaaacgaaga ggttgggttt ggagagaaca cttggaaaat cttgaaatgg
     3601 gaccttttaa atataaagac tgtacaaaac ctttaaagaa gagtgtaggg ctaccatcat
     3661 ctaagtattt caataatata gaccctcaac caagtccagt tattactact gaaatagctt
     3721 ctggtagatt tgaggatgat ataagaagaa tgagaatggc agctcatcat ggagctgacc
     3781 atctaatggt tatcagaact gctggacaat cacattttga tgggcttata gaaggaactc
     3841 cacaaggtat aggtggagtt ccaataacaa gaaaacaagt aagagctcaa aggaaggctc
     3901 ttgattttat agaagaagaa gttggtagac ctattaatta ccattcatat gtaagtggtg
     3961 ttgctggtcc tgaagtagca gtaatgtttg ctgaggaagg agtcaatgga gcacatcaag
     4021 acccacaata taatgttctg tataggaata taaacatggt aaggtctttt gtagatgctt
     4081 gtgaggcaaa gaaaataatg gcttttgcca atatagctca aattgatgga gcacataatg
     4141 ctaatgctac tgctagagaa gcatggaagg taatgcctga acttatggtt caacacgctt
     4201 taaactctat attctcagaa aaaataggta tagataaaag taatatatgc ttatcaacag
     4261 ttccaccaac agcacctcca gcaccatgtt taagaataga cttaccttac gctgttgcac
     4321 ttagagaatt atttagtgat tataagatga gagctcaaat gaatactaaa tatatggaat
     4381 catcaactag agaagcaaca gttactcatg tattaaactt attaacatca gtattaacta
     4441 gagctgacat acaatcaact ataacacctg atgaaggtag aaatgtacct tggcatatat
     4501 acaatataga agcatgtgat acagcaaagc aagctttagt tggtatggat gggcttatgg
     4561 atatgataga acttaaagat gtcggtgaat taagagacaa agcaagagaa cttaaagaaa
     4621 gagctgtatt atatatggaa gaaatatctg aagttggtgg atattttgaa tctgtggaac
     4681 aaggattttt tgttgattct ggaaattatc cagaaagaaa tggtgatgga atatctagaa
     4741 aaataaaagg tggagttggt gaaggtactg tttatgaaag agaagaagat tatttagcac
     4801 ctgtaactgc tcactttgga tacaataatg tagctcaata tgatgaaaat gctatagata
     4861 atccatcaat gttaatagat ggatgtacat ttgaaaatcc agacaaaata atatatattg
     4921 atgagcttga cgattttgat aatgtagaaa atagactaaa agaatcatat gagtacagaa
     4981 atggaactaa aattaagcct gaaatggaat ggtgtgcaga tggaattgta atgataacaa
     5041 tgatgcttcc tactgataaa agaacagcag agtttgcagc tttggaattt gtcaaaaaaa
     5101 tgaacttaca agaaattgag gtcataagta gagaagttat gcatgaatct gaagggacta
     5161 gaatagaagt gaaaggaaga gttccgtttg atatagattt aaacaattta gtaataccag
     5221 aagaacctaa ggtactaact gatgaagaaa taagagcaga tatagaagaa aaacctatga
     5281 aaatagtttc agctacggtt ggagaagatg aacattcagt tggtttaaga gaaataatag
     5341 atataaaaca tggaggtatt gaaaaatatg gcatagaatg ccactatcta gggacttctg
     5401 tacctgtaga aaaattggta gatgcagcga ttgaactgaa tgcagatgca atacttgcat
     5461 ctacaataat aagtcatgat gatattcact ataaaaacat gaaaaaatta cacgattatt
     5521 gtgtagaaaa aggtataaga gataaagtaa tgattgcgtg tggaggaaca caagtaacac
     5581 ctgaaatagc tgttgaacaa ggcatcgatg ctggatttgg aagaaacagc aaaggcatac
     5641 atgtagccac tttcttagtt gaaaaaagaa gagaaatgag taataaataa tggaaaatag
     5701 caaagtttct ttaaaaatag atgtgttggt agctgaaatt ggaagtacta ctacagtggt
     5761 aaacgctttc cacgatatta atactaacaa tcctatattt ttgggtcaag gtcaagctcc
     5821 aacaacagtg tttgaaggtg gcgatgtaag aaatggctta tcaggtgcta ttaaagattt
     5881 agctaataaa ttatctgtag atgatataga gtataatgat atgtttgcaa cttctagtgc
     5941 agctggtgga ttaaaaatga ctgttcatgg attagtctat gatatgacag taaaagcagc
     6001 taaagaagca gctttagggg ctggagctgt tattcgtcaa ataacatcag gtagaattaa
     6061 aagaactgat ttaaataaaa taaaagaaat aaacccaaac ataattttaa tagctggtgg
     6121 agtcgattat ggagaaagag atacagctat ttataatgca gaaatgatag cttctatgaa
     6181 tctaggcata ccagttatat atgctggtaa tgttgaaaat caagaggaaa taaggttaat
     6241 atttgaagat actaattata agctttatat aactgaaaat gtatacccta aaatagattt
     6301 attgaatata gaacctacaa gaagaatcat tcaaagtgta tttgaagaac acataactac
     6361 agcaccagga atgaaataca ttaaagaaat ggttaatgaa aatattactc caaccccagg
     6421 agctgtaatg gaagcttcta aacttttata taaaaatatt ggagatttat taactttgga
     6481 tgtaggtgga gcaactactg atgtacattc tgttacagat ggaagtgact atattaataa
     6541 aattttggtt aatcctgaac ctactgcaaa gagaagtgtt gagggggatt taggtgtata
     6601 tgtaaatatg aagaatatag tagaagttat aggtaaagaa aacttacaaa gtgaactata
     6661 tatagatatt gatgcagtaa tagagaatta tcctccaata ccaaaatcaa aagaagaaat
     6721 attatttgtt gagaggttga caaaagaagc tgttgttaaa gctatgctaa gacattcagg
     6781 aaaaataaga aatatctatg gaacaactgg aaaagtcaaa attgcagaag ggaaagattt
     6841 aacagaagta agatatatag ttggtacagg gggagcactt actaggctcc ctagtagaat
     6901 tgaaatatta gataatatgc tcaaatacaa taaaaataat gaattactat ttccaaagga
     6961 aaaaacaaag atattaatag ataatgacta tattatggca tctatgggtg ttttatctaa
     7021 aaaatatgaa gaagcatctc ttaagctcct gttaaaaagt ttgaattttg aggaggaaag
     7081 attatgtatc ctaggttaga aatagatata gaaaagttaa agcataatgc aaaattaatt
     7141 tcgcagatgt gtcatgaaag aagtattaag atatcttttg ttactaaatc attttgtgca
     7201 caaaaagaaa tagtagaaga aatatgtagt gaaggaatag accatatagc agattcaaga
     7261 atacaaaact taaaaaaatt acaagatata aatctaccta agatattgat tagaatccca
     7321 atgttaagtg aattagaaga agttatcaat tattgcgata ttagttttaa ctcagaatta
     7381 gaaacaataa agaaattaaa tgaattatgt gaaagtaaaa atattattca taagatagtt
     7441 cttatgtttg acttaggcga tttacgagaa ggatactttt atgaagaaga cttttttaat
     7501 aatgtaagag agatagtaag attaaaaaat attgaaataa ttggtatagc tacaaactta
     7561 acctgttatg gagctattat accatctagg gaaaatatag gcagactggt aagtatagca
     7621 aaaagaatgg aagaaaaatt tggaataaat ctagaaatag tatctggagg taattcaagt
     7681 tctattcatc tgttgataaa taataatatg ccagaaggta ttactaattt gagaataggc
     7741 gagtccattc tacttggtag agaaacggca tacggtgaaa atataaatgg aacatatcaa
     7801 gatgcattta aattaatatg ccaagtagtt gaatgtaaag aaaaaccatc agttcctatt
     7861 ggagaaatag gagttgatgc ctttagaaat aaacctgttt atgaagataa gggaatctta
     7921 aaaagggcaa tcatagctat aggtaaacaa gatataaata ttgactcttt aattccaata
     7981 gacactgata taaaaatatt aggggcaagt tcagaccaca tgatactaga tgtaagtaac
     8041 acaaaatatg attataaatt aggtgacaat ttagaatttt tattaactta tgggggtatc
     8101 atgtcatcta gtacaagcaa atatgtttca aaaaaaatta tagtgtagtg tttaaataat
     8161 taggaggaaa ctattttatg aaagagcgag aatgtagaaa agcaagtctg tttgatgcaa
     8221 tgataccaat tacttgtttg atattatttt taagtctagg agtattagtg tatggttcat
     8281 cacctcatgt accactaata ggagcagcag cagtagcagg acttgtggct gtatatagac
     8341 ttggatttaa atggaaagag ttagaattaa gtatgtttaa tagtataaag atggcaatgc
     8401 aagcaatctt aatcataatt ataataggcg tacttatagg aacttggata gtaagtggag
     8461 tagttccatg tatgatttac tggggactaa aaattttatc accaaatatt tttttagtag
     8521 catcaacatt agtctgtgca atagtatctc ttgcgacagg ttcatcttgg agcacaatgg
     8581 gtacagtagg agtagctctt ttaggaattg gtcaaagttt aggtatgcca attggattaa
     8641 tcgtaggttc tataatatct ggagcttact ttggagataa actatcacct ttatctgaaa
     8701 cgactaactt agctccagca atggcaggaa cagatttgtt tacacatata aaatatatgt
     8761 tatattcgac tataccatca ttattaattt gtttagtaat ttatggtgta attggaatga
     8821 agtattcagg tcaagcacta gatataaaac aaatagaatt aattaggtct acattagatt
     8881 ctacatttaa tactttatca ccagtattat tattagctcc agcaatagta attggattag
     8941 ttgtatttaa agttcctgct ataccagggc ttatagtagg tgctgtattg ggagtattgt
     9001 ttgcagtagt ttttcaagga gaatctatga atacgatatt agatgcagca caaaatggat
     9061 atgtttcgtc aacacaaata caggaggtag atgctttatt atctaaaggt gggattatga
     9121 atatgatgtc cactgtatcc ttaacaatat gcgctttatc tcttggaggt attttagaaa
     9181 aaacaggtat gcttgaagta gtagcatctt ctttactaag attagcaaag ggtgtatttg
     9241 gaacagtatt atgtactatg gtaacttgta caattaccaa tataatagca ggtgaacaat
     9301 atctatctat agttatacca ggaagaatgt ataataagga atataaaaag agaggtatac
     9361 atcctaagat gttgtcgaga gctttagaag atagcggaac tttaacttct ccattagtac
     9421 catggaatac atgcggggcg tatataacag caacattagg tgtatctgca ctaacatatg
     9481 gtccatacgc tttattaaat attataaatc caatagtatc tttagtatta attgcatgta
     9541 agtttaaaat agcaagaata gaagatgaac ctgaaacttg tttaaatttt gatgtttgat
     9601 aatagataac ttatgtaaga acgtaggaac taggggggga gcctgtgggg aattagtaag
     9661 acatatttta gtgtgcgaaa ttctataaat cagtaatctt gtgactttag ccatgggagg
     9721 ttcagattag taaatttata gttttatatt agtctatact ttgatctttt aattgtttgt
     9781 ataaattttt tatagaaaaa gatttctttt agacttgcct aaaaattagg caagtctttt
     9841 aagtatgttc tgattataag agcactgtgt gacaagaatg atgagattat ttttgctata
     9901 ctttcgatat aagaaatgga aggaggttat gttttatatt tatatgaaat aattagttac
     9961 acagtgaatt ttcgtataat ttggtataaa aagattagaa atgaaaataa agtaatggag
    10021 gaataaaatg actagaatat ccaatataac aatagctgaa caatctgagt actgtttttt
    10081 ggcaatacga aaaacaattg actttatggt agagttttca gagttttcaa aacaaagttt
    10141 tgaaaaaatt tctaaatatc tggaggagag aggaatttta tcttcaggag caccgattgt
    10201 atgttttcat aacatggatt tagcaaaatt ggatgttgaa gtagggtttc ctgtagccac
    10261 ttatatagat gggaaaaatg agattctaca aaggattgtt cctgcacaga aaataataac
    10321 tgcaattgat ttaggacctt atgaattaca agaccctact ttagaagact tattttcatg
    10381 ggcaaatagc aatggatata aacttcatgg cgatatatat taccagtatt taaatgatat
    10441 caatcgtcca gctagtgaat atcttacgaa aatgatgtta cccattatat agaaagttaa
    10501 agattatcgt ttgttagttg ataattagaa gaacatttaa tgctactgca taaattttat
    10561 cctaaataat atcaaggtga tttaatttat gtattctaat gtaactgtag ataatcaatt
    10621 tataactaac ttagtgtaat ttagatttaa tttattattc taatgtaacc ccaatatatt
    10681 gttaaggtct acttaagatg ctcaaaaggc tatgcttaat ttattgttat ataatttgta
    10741 gagtggtata atttagaagt gatactatat agatatatag ggggaaaata tgaaaatata
    10801 tgatatatta acacgttttt atattcaaga tatagaaaaa gccatacctt tttatgagaa
    10861 tcttttaaaa gaaaaatgtt ctttgcgttt ttcttataaa gaagttgggt tggaattagc
    10921 gcaaatagga aatgttttac tattatctgg ttctgatgat gctcttaaac catttataga
    10981 aacaaaatct acttttatgg ttgattctgt tgatgaatgg agaagttatc tattggataa
    11041 tggagcagtt gtagtaagag ataagaaaaa agttcctaca ggatataata tgacattgag
    11101 acatccagat ggtacaatta tagaatatgt tcaacataca aagcaggatt agttaaaaat
    11161 agattttact actgagcaac taattagaat ctattcttag acgtacaatt tattattttt
    11221 ggtattaata gattataagt caaattataa tatttcaaaa tagaatagaa atttatttac
    11281 ataaaagaga gtgtttaaaa atgaactttt tagttcacga agcactcctt tttgtatgaa
    11341 atcaaataca tcaatatata aaacaaactg atgaagagct taaattaaaa taaaaagatt
    11401 cataacagga ccccattaaa ctttaagata gggtaagttt gtaatcatat taaataattg
    11461 taaaaagctg tttttagtcg tataatataa atatttatca tttaattaag gaatctaaaa
    11521 ggaatctaat aagaagaaaa taataaataa ctttttagat ttactaaatg aaaaattatt
    11581 gaaaattaaa ggattagagt ctttaatcag aaagtaaaaa gtaatatatt aaatttatga
    11641 attgaaaact taaacaacaa atttgtttat aaaatagcaa gattcattaa atataactaa
    11701 gtaataattg atataatgtt gcatggataa aaggagggaa gctgcaatgt cttacaaagt
    11761 aattaatgtc ataactgcta ttgactatat agaggagcat ttatcagaaa agctggattt
    11821 agatataatt gctaatgctg ttcattactc aaaatatcac ctgcatagaa ctttcacaac
    11881 ttctgttggt ctaacaatgc atgattacat aaagcggaga aagttgacag aagcagcaaa
    11941 attattggta ttttcaaaga aaccaattat tgaaattgca ttgatagctg gatatgaaag
    12001 tcaacaggca ttcacttcta tatttaaagc tatgtataaa aaatctccaa ataaatatag
    12061 gaaagaacaa gaattttatc ctttgcagct aagatttgtt ttgaaaaaag ataacctatt
    12121 ttcaaagaat gtactagagt tggaaaaaga aattaaactt gcaagtatgt ctgacatacc
    12181 attatggatg aatttagtga gattagtcat agatggattt ccaaatttgc aagaggaaga
    12241 atatatatat caattagaac agtatatttt agaagaaaga gctttaatat taaaacttaa
    12301 taatgtcgct attgcaaata tggtgtttaa tagagaaact agaagtattg atttttttgg
    12361 aatacatcca cagtatcgta attcagacat tgcacaagtg tttttgaaga aagttattga
    12421 agatttcttg atagatacag atatctcaat tactaccttt cgagagggtg acaaagctga
    12481 tacaggacat agagatatga taaagaagtt ggggtttgca gaagcagagt tgcttgtgga
    12541 atttggatat cctactcaaa aatttattct tccttgtgta gaagaaagag ataatagaca
    12601 aaattaattg tagattaaat gcaaagttta ccacatattt tttgtttgaa ttattttata
    12661 tataagagta tttctgcttt taatgtattt tgcttgtaag tgtacttatg gtaataaata
    12721 tagaaagatt tagtttttta aaaaaagaac tttaaagcta aaaagctaac atttaagtta
    12781 aatagcaaga atgattaaaa actccaaaac acaattaagt ataatgattc agtgtaagta
    12841 cttaattaac aaaggaggat ttaaatgtct gaaaataaac aagtagtaaa ttgcgatatt
    12901 attgcatcca attctaaaca taatatgtgt gaacatgatg tagagttatt aacttttttt
    12961 ccagatgaga ttgcacatct aatagagata aataacaagc tagacaatgc ttttaagaaa
    13021 gctgaaaatc ttgttgacaa gcttgataag gattatatgg acgctaaaat gtatatggta
    13081 aaaaatcgtg gtgaaataga cccacatgaa atgtttcaaa atgaacaagg actaaaacaa
    13141 atagataact atggggcttt tatggtaaaa gttcgagata agattaataa aatcaaagat
    13201 tctccatact ttgctagaat agattttagg ctgaaagata tggatgatga atctaagtat
    13261 tatattggta gatttgcatt tgattatgaa gatgagctta taatacttga ctggcgttct
    13321 ccaattgcca gtatgtttta tgattatgaa atcggaaaag ctggatatga tgctccaata
    13381 ggatgggtgg atggagaaat aacaagaaaa cgacaattta aaattaaaaa tggaaaattg
    13441 gaatatgcac tagaaagttc cattaatata caagatgata ttcttcaaaa agaacttagt
    13501 cacacttcag atgaaaaaat gaagtcaatc atttcaacaa tacaaaagga acaaaatcaa
    13561 attattagaa atgataaagc tgatacactg attattcaag gggtagctgg ttctgggaaa
    13621 acatctatag cattacacag aattgctttt cttctttatc gatttaaaga taaaatatca
    13681 gcaaataatg taattatcct atcacctaat aaagtctttg gagactatat ttccaatgtc
    13741 cttcctgagc ttggagaaga accattatgt gaattaagtt ttgaaaatat agcagaggta
    13801 caattagata gagttattaa ttttgaaagt gagaaagacc cattagaaat aaatgatgct
    13861 aaatggtctg aaagagtgcg ttttaaatct acacttgatt ttgtaaaact tattgatgat
    13921 tatataaaac aaatgccaaa taagattttt attccaaaag actacacttt tggaagtttt
    13981 acagctaaaa gtgactggat acagagtaga tttgaggcat ataacagata tccagttaag
    14041 aagagattag aaaaggtagc tgaagacatt cattataaat ttgaatcaga taatattatg
    14101 gaagaagatt taccaaaggt aaaaagtata cttaaaagtt tgaatggaat gttgacaata
    14161 aaaaatactc ttacactata taaggatttc tttaaacaaa tgaatatatc aaacatgttt
    14221 gtgatggcag aaaagaaaac acttgaatgg tcagatgtat acccatttat atatattcat
    14281 gctgcatatg aaggtataca agaagataag attataaggc atattgttat tgatgaaatg
    14341 caggactata cacctattca atatgctgta ataaacttac tttttaagtg taaaaagact
    14401 attcttggag attttggaca attggttaat cctaatcata ctcatacatt agatgatatg
    14461 aaacagctat ataatgatgg ggaactagtt atgttgaaca agagttaccg ttctactttt
    14521 gagattatta atttcgccaa aaagattcaa gatatatctt cattagagcc tattgaacga
    14581 catggtgaag aaccggcttt attaaaatgt aataacgagc aagatgaaat aaataagata
    14641 aaaatagaaa ttgaagaatt taaaaagagt gataatgcga ctcttggtat tatattaaag
    14701 acagataatg acgctaaagc tgtatatgat gcgttaagag aagaatatag tgttcactta
    14761 atatcttctg aaagttctag ctttacaaaa ggagtatcca taacttctat taaaatgtca
    14821 aaaggtttag agtttgatga agttattgta ccttcagtaa ataataaaac atattatagt
    14881 gattatgacc gcagtttgtt atatattgct tgtacacgag ctatgcacaa acttaagtta
    14941 acatatgttg gtacattaac tcaacttata agcatgtaga ttatatctta tatgcattta
    15001 ttatcgtaaa tataaattgg gacaaagatt catcaataaa tcaacaaaaa ataatactta
    15061 taaatttata aaaaatgaaa gctataaatt acatttgaaa ttttataaaa ttttgtgatt
    15121 tatagctttt ataatattaa tttcttttaa aataaagtta aactttaaat ttatgaaata
    15181 aaacaaagta gtaatatata tgtaatagtt cttaattttt taatattagt attatattat
    15241 ctatatattt attgattttc taaggaaatc tgatgttata gtgcaacctc tttcaaacat
    15301 ttctagtggt gtaccttcaa atacgacctg accaccgttt ttgccaccat caggaccaat
    15361 atcaattacc cagtctgctt gtttcaatac atccaaatta tgttcaatta taattacagt
    15421 attaccttta tcaactatat gctcaaaaat caacataagc ttttcaatat cagaaccatg
    15481 aagccctgta gttggctcat ctaaaatata tatagtacct tttttgttga ggtttttagc
    15541 taacttaatt cgttgacgtt ctcctccaga tagtgtgcta agtggttgac ctaaggtcat
    15601 ataaggtaaa ccaacatctt tcattgtttg taagatttta catagcttct tttctgtaaa
    15661 aaattcaaga gattcatcta ctgtcataga tagtacatca actatacatt tacctttata
    15721 taattttgac aaaacttctt cactatatct agtacctttg caagcttcac atgtattaat
    15781 aatagggtcc atgaatacaa gctctgtaac aagtactcct ttaccatcac aaacaggaca
    15841 agctcccttt gaattaaagc tgaataaacc ttcttcaaca ttattttctt tagcaaatac
    15901 ttttcttatc tcatcaaaga aaccaagaaa tgtagcaggc atagaacgat ttgtagctgt
    15961 aactgggctt tggtcgattt taataacatc tttttcgtat ttttgtgcaa aaacacgaga
    16021 gattaaagta ctcttgccag aacctgctac tccagtaacg acagtcataa ttccaactgg
    16081 taaatctaca tcaatatgtt ttagattatt tatgcaagca tctctaattg gaagggtacc
    16141 ttttggatga cgagagtcag tttttatagg taatgataga ttcattgcat ttgaagtaag
    16201 agtgtttgct aaaagtaatt tatcataaga gccttggaat acaacatgtc caccctgagt
    16261 accagcactt gggccaatat ctataacttc atctgctatg gagataacat ctttatcatg
    16321 ctctactaca aggacagtat ttcctctatc tctaagtttt tttaatagct gattcatacg
    16381 atgtacatct cgtggatgca taccagcact aggttcatcg aaaatataag tcatacctgt
    16441 gagactgctc cccatatagc gtacaagttt taatctttga gcttctcctc cagatagaga
    16501 tgatgattct ctatttagat gtaggtaagg gagaccaata tcaatcattc tttttaaacc
    16561 atcagtaaga gtctttatta cagtttcagt tcttttatct tttatttgta acaataaatc
    16621 ataaaggtca gtaagttcca tattgcacat atctgcaatc gaatatccta aaatattaca
    16681 gttaagagtt tctttattta atctttttcc atgacataca ggacattcct tatctgttat
    16741 aagtctttct gatttttctt ttgtgtgttt gctcatattg ctaatatctc gatttagata
    16801 tgtttgattg tatttgttaa aaataccctc tatttttgga ttttcttgtt ttccatcttt
    16861 attttttgaa ccataaagca ataaattata ttcttcaaaa gaataatctt ttataggttt
    16921 atctaaatca aaaagtccag actctgcata ctgcttccag taccatgaat taggtttaaa
    16981 taaagagtca tttatacatc ctgtattcca tgaattttca ggatttataa tagattcaat
    17041 attgattact ttaactctac caatcccaga acaattttta cacataccat ttgggtcatt
    17101 aaaagaaaaa tatgaagttg ttcctatgta gggttctcca attctagaaa ataataatct
    17161 taatgatgaa tacaaatcac ttatagtgcc aactgtagaa cgtgcattgc caccaagagg
    17221 agtttggtct acgattacag aagcagttaa attatcaatc agctccacct gaggttttct
    17281 atatttaggt agcctatttc tgataaatga agaatatgtt tcattcatct gcctttgtga
    17341 ttctgctgca attgtatcaa atacgatact ggattttcca gaaccagaaa ctccagtaaa
    17401 aacaacaatt ttatttttag gaattttaaa tgatatattt tttaggttat tttgtgtaag
    17461 tccttttatt ataatatttg acatgaatgt taaatccttt ctgagtacat tttagtaaag
    17521 caattatatt atgtaaccat attataataa aattaaaatc tactttcttg atatattctg
    17581 ctatgttata gaaggtattt ctaaatttaa ccaatcccat acaatctgaa taaatgatag
    17641 ttgataatag ataaaaaagt atataataga tactcgtaat ttgataagta caaagataat
    17701 tatacaatta aaacgacttg acatttaaaa cttactattg taatatttaa atattacaat
    17761 agtaagtttt aaatagagaa taaataatta cctcacaaac tgaaaggagg acataatgat
    17821 actaatagtt atattgagta tattcatcat agcctcaatc aacataatta tattaggtaa
    17881 agaaaatata agagatatag ttatcccttt aactctattt attgtaagtg attttttatt
    17941 tttgtgtgga aatataatat gtaggggagg agtaatgcta ctatttatag gtttatttgc
    18001 aataccattg atgctaattg catttatatg gatttgtgta catatgatta agctttacta
    18061 taaaaaagat ttaaataaaa gaaaaatgat tggatgtata tttagtatct ttataagtat
    18121 aggtactata tttattccta gtccaagtga aagttttaga tttaaacttt atgaaaaaga
    18181 ttatagtgta gtagctaaag ctattttgaa agtttatgat gaaaaaaagt taagctctga
    18241 atctgtcttt tatattcaat ctgataaaaa tgaattggag aagatatttt ctaaagaagt
    18301 aataaaaaaa atgaaaaaac taaacagaaa tttagatgta gttaaaattt ctgggaatag
    18361 aaatttagtc tattttttct ttggagctga attacaatct attgatggta ttgtgatttt
    18421 taaagattta aaacatgtta aaccaaagtt agattttata aatagaagta ttcaagctcc
    18481 tagattggag tatattacaa ataatgtata ttattttaaa ggtgaactat aaacttatgt
    18541 agaggagatg gtataaagta tgaaaagaaa aaataatttt atttggatag ttattcttct
    18601 agttgttgta gctatgggag tatattatgt aggaagaaag cataacaaca caaaacaaaa
    18661 acatgataag aatcatataa actctgaatt aaataaatcc aaagttgaaa acaatgataa
    18721 aaacaaaaaa gaaaaaatta atatggttga ctatagtgac tgttttgaag gtattagtgg
    18781 aggagctatt ttttataata ctaaaaataa agaatataat atatataaca aagaattaat
    18841 tgaaacaaga aggtcaccat gttctacttt taaaatagtt tcaactttaa ttggtttaga
    18901 aaaaggggta ataaactcaa aagaatcagt tatgggatat gatggtacag agtatccaaa
    18961 taaaaattgg aataaaaatc ttagtttaga agaggcattt aaggagtctt gtgtttggta
    19021 ctacaaaaaa ttgatagata aagttgatgc taaatctgtc caaaacattt tagatgattt
    19081 aaagtatggg aattgcgata ttagcgaatg ggaaggagat ttaaaaaatg gaaagggaca
    19141 tttaaatgga ttttggttag aatcttcatt acaaatatct ccaaaagaac aagtgcaaac
    19201 aatggcaaaa atatttgagg gagatacaaa ctttaaaaaa gagcatatta atattttaag
    19261 agatattatg aaaatagatg taaatgataa aaacataaat gtatatggaa aaactggaac
    19321 aggatttgat gaaaaaaata aacgtgtgga tgcatggttt gttggtatgc tagagcgtga
    19381 aggtgacacc tattattttg ctattaaatc agatgattcc aataaagaaa ttacagggcc
    19441 aaaggtaaag gaaattgcaa ttaatataat taagaaatat tatagtgtaa gggaataaac
    19501 ttaattttat ttgtaaatat tatttaccta tagtctgtaa tatttattaa ataattttac
    19561 taaattgagt ctatatagag caaaactcct ttagtacata aaatacttgc tattgattat
    19621 taaattgaat acctatagat ttaaagttaa taccttttaa agactatgta atatattaaa
    19681 agtataaata aaatatattg catagttttt gttttatatt ataattttaa ataatgtgca
    19741 aaaactaaac aatattttta atagtaattt atatttgtat tcatgtcact ataatgtcat
    19801 tttaggttgc taaaatttaa ccataaagga gatgattgta tatggaaata ttaaaatgtg
    19861 aaaatctcac aaaaatatat ggttcaaatc aaaccagagt gacagccctt aataatgtaa
    19921 atttatcagt tcaaaaaggt gattttgtat ccattgtagg agcatctggt tctggcaaat
    19981 ctacattgtt acatctgctt gggggagtag atagacctac atctggaaag atttatgttg
    20041 aagatacaga aatatcaagt ttaaaggaag aagctcttgc agtttttaga cgtagaaaag
    20101 taggtcttat ttatcaattt tataatctta tacctaccct tgatgttaga aaaaatatat
    20161 tattacccat gttacttgat aaaagaaagg tggatgagga tagattctct gagattgtat
    20221 ctatattggg attatctgat agattaaacc atcttccaag tcaactttca ggtggacaac
    20281 aacaaagggt atctatagct agaagtttaa tctatagacc agctatttta ttagcagatg
    20341 agccaactgg taatcttgat agaaaaaatt cagaagaaat tgtagacttg ttaaacttat
    20401 caaataaacg atttaatcag acaatacttc ttattactca tgatgagaaa atagcactag
    20461 aggcaaatcg tattgtaact atggaagatg gagtgattgt ctctgaaaag gtggtgaaga
    20521 aatgaatatt cttcaagaat acaccttaga ctttgtacga cgcaataaac gtagtagtat
    20581 agctataatg ttagctatat tacttactac aaccatgatg tcttctttgt gtggactgtt
    20641 gtataccatg tggacagatt ttataagact ttcaattgag aaagaaggta actggcatgg
    20701 tgaattgttt gactatactt atggaagtga cttaccatta attgaaaatt tttcttcagt
    20761 tgatgatgta atggtaaaag gacaatggta tgtaggcaaa attaatgatg agaaaagaga
    20821 ttatataatt tatagaaatg ctacatcaga gtattggaat tctatgccag aaaaggatac
    20881 tataatagag ggtcgtcgac caaataatgc taatgaaatt gcattatcaa aacaatattt
    20941 tgagaataat ccaagtgtaa aaattgggga taaaattact cttccaatag gagatagaat
    21001 cctaaatgga aagcctcttg aacccatagc tcaaaaatcg aaaggggaaa gttttaaaaa
    21061 aatttcagag actacattga caatagtagg aaaggtagat gtttccactt catcagtaat
    21121 acctgcatat acagctcttg gatatcttga tagaacaagt ataaaaccta atgatagtat
    21181 aactgtgtac ttgcgattta aaaatataag agacacatac aaggaacttc caaaacttgc
    21241 taaatcattg gggtggaaga ctaatgaata tggaaaatac aatcttaaat ataatagtaa
    21301 ttatctttta aaaatgttag tcttttcccc agaacagaaa gcatctatga gcagtatatc
    21361 aaaattttca actcctatta tgtatcttac aatagcaatt tttacagtag ctgtgtttgt
    21421 aatggtgatt tacaacgcgt tttcactatc tgcaaatgct agacttactc aattagggat
    21481 attatctagt gttggtgcat caccaaaaca gattaaaatg tctgtagtgt ttgaaggttt
    21541 tttgcttaca attataccat tgccaatagg tttattttta ggatggttat tgtgtaatag
    21601 gcttatagtt tatattaatt cagttaatta tcatactgat gttccagaag tggtatttac
    21661 ttatggtatt cctgcttttc tgccagcagt tttacttaca atagtaacag tctggatttc
    21721 tgcacttata ccagcacgaa aagtatcaaa gatatctcct atagaagcaa tacgacaagg
    21781 agatagtgtt aaaatcaaaa agtcgcataa atattcatta ggaaagatat ttggaataga
    21841 aggagaactt gcatcaaacg cactacgtgc tcgtaaaaaa tcttatcgta ctgcaacaat
    21901 atctttaaca ttatcttttt tattacttac atgttttttg catataattg caaatcaaga
    21961 aggagcaaaa gctgtttatg gaagtgataa ttataaggac caaagagata tagcagttta
    22021 tttggaaaat agtgaacctg tagagtctgc tttcattgaa aaattagata atacagaagg
    22081 tgtaaaaagt tcactatatt atcaaaaaat gtctgcttcc acttggctga ctgaaaaaga
    22141 tgcatcaaaa gaacttgcga aaaatggagg atttaaaagt atagtttcat ctaaaaaata
    22201 ctctcctata gaacgtgatg gcaagtttcg tataatttca aatataattg ggcttgatga
    22261 taagagtttt acagaatatt gtaggcaaat ttctgtagac ccaaagttat tttatgatac
    22321 aaaaaatcca acctcaattg tatataataa ggaggaagat attaatcgta gtacacgtag
    22381 aaataaagta tatatagatt atttaaatct ttcaatagga gataacatta aattaaatga
    22441 aaaagtatat gacgaagata aaggggatta tacttacaac atgaaagtgg gagctattac
    22501 aagcacactt cctaaaacag tagatactag ttcaaaatat acattaatgc aaatagtgcc
    22561 aatgtctatg gtgcaggaaa tatctaaaaa ttatgatgaa gtaagtaggt tacgttcaaa
    22621 taggctgata ggagtattta aagtaaataa tcgtgaggac attcctagag taagagaaaa
    22681 attagagtcc atttataata aagactatgg ttcaggagat tatagtattg aggatgtttt
    22741 agaaagtgaa aaacaaaagg ctagtggaat aaagaccatg aatttaatag taggctttat
    22801 tacaggttta ttggctatga ttggattatc aaatgtactt gctacagtgt caggaaatac
    22861 tagaagtaga cgacaagagt ttgctatgtt gcgttctgtt ggattatctc cagaaggcat
    22921 aaaaaagatg cttgttttgg aaggtttgtt cttaggaatt acaccattac tacttagtat
    22981 tcctgtgcaa ataggaatag tgtatgcttt tctacgtata aatgaaatat attttataga
    23041 gtatttacca tttgcaccta tatctacaat aataggtttt actatattga tactttttat
    23101 tgtaatagca tcatatatga caggatacaa gcagttgaag aatgaaaata tagtagaatc
    23161 tataaaaaat gaaacaatat aaacaaaagc tatttgaact tatttaaagg tttcgattta
    23221 agactactta caagtatagg gatagttaat ttgtttgttt aataaaacct taaatgtaaa
    23281 tagaagtata ttactaaaac aatagataac atagaatata gatatagctg gattataggt
    23341 attttaccta taatccagca ttttatgtgt aaaatttaat tatgaatctt gcaccaccat
    23401 tatggcaatt ttctgcatgt agagtaccat tttgacgttc tataatagac tttgatagag
    23461 caagccctat accaatactg tccttactag catttttacc acgataaaat ctattaaaaa
    23521 tatatggaag ttcttcctga gcaaaccctt taccattatc ttctatgata atctcaacat
    23581 atagaggatt ttgactataa taagcaacta tatatccacc atcttctata tgctcactac
    23641 aatttttaag aatatttaaa agagcttcta aagtccaatt aatatcgatt tcaaattcta
    23701 ccgaaggaga agcctcgata gtaaatgtct gatgtttttc tcttaaagaa ccttctatag
    23761 gctcaattgc ataagtaaga agagaatgta catctataag tttgctttca aatattaaag
    23821 tacttgcatc caattttgat aatgtaagta aagatgaaac tagtctctca agtcttgaga
    23881 tttgatttgt aagtctgtta atataatcta tttcttcatc tggacaattt tctgaaagta
    23941 attgtgccat aagtgacata gaagtaattg gagtcttaag ttgatgagca atatctgcaa
    24001 gattatctga aagagtttgt ctttctttta aagcaaattc ttttgaacaa cgaagttccc
    24061 caactgtttt atatatctca tcttcaaggt gagaaaattc atcttcacat cgtaaaagta
    24121 cagactcttt tcctaaattc acttcttcta agtattgagt aagattgttt attctactca
    24181 gtttttgctt atttgttcta tgtttgatta tatatacaac tgtagccatt gttgaaatta
    24241 taccaaacga tatcactaga aaataaaaaa agtttttact ccaaaatgta cttttagaat
    24301 atccatgttt tgataaaatt tcatttcctg ttttaaaatc cttatcacta gaatttttca
    24361 gtgattttag tatactttct gtagacttag gattgtactc atatgatgca ccaagagcct
    24421 tagacagtac attgtattca ttaaaagaag ttatagttgt tagaattaca cttaataaaa
    24481 aagttataat taagcagaat attataaaaa caactgtact gttgatattt cttggagttt
    24541 tcactagctg tcctccattc tatatcctat gcctcttaat gtttttatac aaggtggatt
    24601 ttttaatttt tctctaagac gctttattgt aacagtaaga gtgttatcat ttacgaaatt
    24661 accatctaca tcccagagca tttctagtaa ctttgtacgg gtcatagctc tatttttatt
    24721 ttccataaga cttagaagaa gtttatattc tcctgctgtc aaaagtactt caacattatc
    24781 taaatatgct ttgacttcaa ctttattcaa gcttatattt ccacatgtaa tagtattgtt
    24841 agtatttgaa tgagttcttc taagaactgc attaattctg gattgaagta ctgagagcag
    24901 aaatggttta accacatagt catctgcacc catatctaat ccacgtataa tgtctttttc
    24961 atcatcacgg actgtcaaaa atatcactgg agtatcttct atacttttaa catattggca
    25021 aaactcgtat ccaataccat caggtagatt taaatcaagc agaattaaat caatagtttt
    25081 atcaaaattt tttttagctt cagcaatcgt attataacaa catacagtgt gatttttctt
    25141 ttcaagggaa gtttttattc caaatgtaat tgtatcatca tcttctaaaa gaagtatttt
    25201 agccatttac ttttagtcct ttcagtaatt tagttatttc attaactaag ttattagttt
    25261 tatgtttaat attttttatg ttagagatag aagttagaga tagaaaaaat tatcatttaa
    25321 gtatataaac tttaatttat aaacttaggt ttatgcttta attgaatcta ttaaaagagc
    25381 aagagttgtt tcaataaatt tttcctcatc tatattaaag ttgttcaggt atgactttcc
    25441 tgttacagat agtaaattaa aaaatcctgt aagtgtaaat attgatgaat acatgagagt
    25501 aggtatttca atatcatgcc taatacttct atcatttttt ccaatttcaa acattccttg
    25561 tatttcatca aacataaatt tattaaaaga aaaaaacttt tctttaaaca gcatatcagt
    25621 atccgatttt tcagagttta caataccaat cattcccata aggcataata gagaaggttc
    25681 tacagagtaa aatttatgaa aagcaaagta tgaaagtttt attttttcaa accctgtttt
    25741 gccttttgca ctttctattt taacattatc ccaaagtctt ttatatcctc ttaagacaac
    25801 agcaaagaat aaatcttctt tacatgaaaa gtacttgtat atggtgcgtt tagtatattc
    25861 aacttctttt gccaactcat tcatagttgt attctgaacc tcccatgact aaagtcacag
    25921 ggttcctgct tcatagaatt ttgcatacta aaatatgtct tactaattct ccacaggcca
    25981 tccccgtagt tcctacggtt cttacataag ttatt
//
LOCUS       DAGQWR010000050        25976 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.050, whole genome shotgun sequence.
ACCESSION   DAGQWR010000050 DAGQWR010000000
VERSION     DAGQWR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.050"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(330..740)
                     /locus_tag="MIW95_001772"
     CDS             complement(330..740)
                     /locus_tag="MIW95_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151100.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(956..2338)
                     /locus_tag="MIW95_001773"
     CDS             complement(956..2338)
                     /locus_tag="MIW95_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBY3151101.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKSGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIEQSNYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2349..2708)
                     /locus_tag="MIW95_001774"
     CDS             complement(2349..2708)
                     /locus_tag="MIW95_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY3151102.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(2913..3692)
                     /locus_tag="MIW95_001775"
     CDS             complement(2913..3692)
                     /locus_tag="MIW95_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBY3151103.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
     gene            complement(4153..4764)
                     /locus_tag="MIW95_001776"
     CDS             complement(4153..4764)
                     /locus_tag="MIW95_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBY3151104.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            complement(4856..5845)
                     /locus_tag="MIW95_001777"
     CDS             complement(4856..5845)
                     /locus_tag="MIW95_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBY3151105.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NRESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            complement(5912..6775)
                     /locus_tag="MIW95_001778"
     CDS             complement(5912..6775)
                     /locus_tag="MIW95_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBY3151106.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            complement(6881..7477)
                     /locus_tag="MIW95_001779"
     CDS             complement(6881..7477)
                     /locus_tag="MIW95_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBY3151107.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGLFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            complement(7517..8320)
                     /locus_tag="MIW95_001780"
     CDS             complement(7517..8320)
                     /locus_tag="MIW95_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBY3151108.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKSFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            complement(8356..8796)
                     /locus_tag="MIW95_001781"
     CDS             complement(8356..8796)
                     /locus_tag="MIW95_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBY3151109.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            complement(8809..10911)
                     /locus_tag="MIW95_001782"
     CDS             complement(8809..10911)
                     /locus_tag="MIW95_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBY3151110.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEAIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKNVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQINEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            complement(11168..12871)
                     /locus_tag="MIW95_001783"
     CDS             complement(11168..12871)
                     /locus_tag="MIW95_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBY3151111.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINRV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            complement(12897..13856)
                     /locus_tag="MIW95_001784"
     CDS             complement(12897..13856)
                     /locus_tag="MIW95_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY3151112.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNTRTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            complement(13992..14510)
                     /locus_tag="MIW95_001785"
     CDS             complement(13992..14510)
                     /locus_tag="MIW95_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBY3151113.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            complement(14553..15038)
                     /locus_tag="MIW95_001786"
     CDS             complement(14553..15038)
                     /locus_tag="MIW95_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBY3151114.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            complement(15035..15652)
                     /locus_tag="MIW95_001787"
     CDS             complement(15035..15652)
                     /locus_tag="MIW95_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBY3151115.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            complement(15718..16080)
                     /locus_tag="MIW95_001788"
     CDS             complement(15718..16080)
                     /locus_tag="MIW95_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBY3151116.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
     gene            complement(16824..17321)
                     /locus_tag="MIW95_001789"
     CDS             complement(16824..17321)
                     /locus_tag="MIW95_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBY3151117.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNAIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVILAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            complement(17496..18407)
                     /locus_tag="MIW95_001790"
     CDS             complement(17496..18407)
                     /locus_tag="MIW95_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBY3151118.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            19332..20183
                     /locus_tag="MIW95_001791"
     CDS             19332..20183
                     /locus_tag="MIW95_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBY3151119.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            20187..20657
                     /locus_tag="MIW95_001792"
     CDS             20187..20657
                     /locus_tag="MIW95_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151120.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            20670..21980
                     /locus_tag="MIW95_001793"
     CDS             20670..21980
                     /locus_tag="MIW95_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY3151121.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            22192..22938
                     /locus_tag="MIW95_001794"
     CDS             22192..22938
                     /locus_tag="MIW95_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY3151122.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCNSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            23300..24841
                     /locus_tag="MIW95_001795"
     CDS             23300..24841
                     /locus_tag="MIW95_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBY3151123.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            24876..>25976
                     /locus_tag="MIW95_001796"
     CDS             24876..>25976
                     /locus_tag="MIW95_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBY3151124.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVTTEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DPLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIK"
BASE COUNT         8568 a         4347 c         2984 g        10077 t
ORIGIN      
        1 gttaacatta taaatttggt ttaatacttt tactttgact cgatttactt ttattctact
       61 tcttacttta tttttaactt agactttatt tttttattac ttatttgagt cttatttatt
      121 ttattctcac tccatatgtt caattacatt tgtctaaatt ctttgatact tttattacat
      181 aattttaaca agcttaagtg ttatttgggt agtgcagttg ataattattt aaataatcat
      241 caactgcatg tttatatcat tacagtagct taatgtattt aatcatactt acttaggatt
      301 ttgaaatatc ttagagatta tatttaacat tagtcaattg ctgcataatt gtaagcaatt
      361 gaaatttgag acccatcttc attttctata gtgataggct tcataatttt taatccttca
      421 gtttttatct tttctaattc ttgttccatt ttcttaattg tttcctcaag gtctttttga
      481 cttacatctt tgactgcatt agggtcttta gcatagtctt ttaattcttg aatatgttta
      541 gcatactctt cataagtata atgctcaaca ccatactttt ccaattcagt agggtcagtt
      601 attcctgatt ttattagaga ttcaagttta ggtaaagcaa tattagatga atcatttcca
      661 tttgcaaata ctgctccagt tcctcctagt accattgcag ataccatcat tgcacttata
      721 agggattttt ttattttcat aaccaatctt cctttcttag agatttattt gggtagtgta
      781 gtttaaaatg actattgcaa tagttatcaa ccacatcttt agactaccat agtagtcata
      841 tttgtgtcaa gtctttttaa aaaacttttc aataaaaaat actatctcaa aacaaaatta
      901 attattttga gatagtattt taattctaaa tcttttatat tactaagatt tatggttact
      961 ctatccatgt tctatattgt tctttattaa ctgctaacaa attccctttt cttgatatga
     1021 caaagcgatt atcattatag ttactttgct caattgaaac ttctcctgaa tcagtataaa
     1081 gtacatcctt actattacta gatttattta catacgtttc ttcactattc tcagaattag
     1141 ttactttgct catagatgat tcttcatgct ctctaaattc aattacagat acttttgccg
     1201 tactaaaatg agtctctcca tcacttttag ctttactaac aagaatatca gattctactt
     1261 tgggagaata taaatcagtt gatttactac ttattgaaaa tccactttta tcactttgtt
     1321 cattttttgt taaaccaaaa gattcaacct cattaatctc tttattttct gttgcaaaaa
     1381 caagacctgt cccacaaact aatacagttg aaactaaaaa agtaacataa gttgattttt
     1441 taaatttcat aatattaact attctctttt ctatttttgt ctttccaaat ccactacata
     1501 ttgtcattga aaatggatta ttctcctctg ctagacttat tagtgtggtt gcataatcag
     1561 ccctatattc ttcaccaagt atactcataa ccttttcatc acaactaaat tctatgtctt
     1621 tatttagtaa aaaaaacatc acccaaacta agggattaaa ccaatggaca acaagagcta
     1681 caagcataaa taactttaac agattatcaa agctattaat atgaaccatt tcatgagtta
     1741 aaacaaagtc taaagtttga ttattagcat aattcatttg ctttggcaaa ataattcgtg
     1801 gttttaaaat accagatgta agtggtgtcc tagtaaattc agatatgcaa acaataaatc
     1861 ttctaaaacg gctattctta cacatccatt ttttaatgaa ctcatgattt tttatatgaa
     1921 ttgaagtttt aaaaagatta attattttaa aatatctaaa gcataaaagt atgctaaaca
     1981 taatagtaac tataaaagac aaaatgccca acatttccaa catcataata ttttcaatat
     2041 tagacattat ttcaaattcg tcagaattgt ttttacctgt agagtcacta gctttcttaa
     2101 gagtataatt ttctgttgat gtattaccca attctacatc aaaagtcgat tcaattatac
     2161 tataaatact aaaaccagaa tttaaactta ctggtattag aagtttaaat aaaacaatca
     2221 accacaataa tacaaatacc tttttaggta attttttaaa taaacatgct cttataaaaa
     2281 taactattaa aagtaatact ccagaagtaa aactcatttc taacaattta atgaacataa
     2341 atcttacctc actttaattt ttcaacaatt tgtttgagtt ttgtaatctc atcacttgaa
     2401 agattttctt tattaataaa agctgaaaga aatttttctg aagaccctcc aaacatttta
     2461 ttaattaaag tttctgtttc ctgtttttgt acttcctctt tgcttattaa agctttacaa
     2521 acaaaatttg gctcaattct ctctattgca ccttttgaca ctaatttttt tataacggta
     2581 tacgtagtgt ttctattcca tcctgtttct tcttttaaaa tttttactaa ctctccagcc
     2641 ttgctttctc ctctctccca tagaacttcc attactttaa gttctgaatc aaataatttt
     2701 atctccatat aaaatcgctc ctcaaatata gttatcacaa tagtcattac tataataaaa
     2761 aactatcata gtagtctata ctatgtcaat catttatttt ttaataataa aaactgtctt
     2821 tttaaaaaat tcttataaaa gacagtttta tcaatgttat actttaatgt ttaattaaag
     2881 ccatatttta tttaaatgta taactatcta tcttagtaca tagtataaga tttttcttta
     2941 tctaatagta tatcctctac atctgggttt tcttttacta attgtctata aaaattacaa
     3001 ttagtaacac ctacatatgg aattagccat agtagaccta ttccaagagt agctattgaa
     3061 agtatatacc atcctataaa actaaagttt agtacaaata atttaagtac atttcctttc
     3121 atcatatcta aactagcacc cattgattta aatacaccca tattatcctt gtcttcacaa
     3181 attaagaaag tagcttgaga aacatataat agcaatatat aaaccggtaa cataagaaca
     3241 agtattataa gaactagagt tattccagta gaaataatag tccctattcc aacattatca
     3301 cttgataaat attcaattgc tccactagta agtaaaactc caaatgataa aaacgctcct
     3361 ataattgtga ttataaaaga ataaatataa acaataattg attcaaataa ataaaaacca
     3421 aaacttctta aataaattct cccacttaca aagatatctc ctatcttgac tttttcatct
     3481 ttagtaattt tcaagaacat acatgtaaat cctgctgaaa cgcaaaaagc tattacagcc
     3541 actacaatag tagtcaatgg ttcaaaaggt aatcctacaa ttgatattaa tactatcaat
     3601 gcaatatata ctgccataga aattgcacat attccccagt ttccagataa ctgagcttta
     3661 gatactcgtt ttaattcttg ccttgatacc ataaaaaaca cccctttcat tttataaaaa
     3721 aacttttaaa aatataaata atcttttaac gtataatatt aatatatcat attttcgact
     3781 atataagata caattattca ccatgtttta tcaagatatg ccataattgt attactagtt
     3841 ttatatagtc aacattacca aatacatcaa ataaatttga ttaattattc aacaagtaat
     3901 aaaataattt ctattaattg tactttattt agtaaacaaa ttatttaata aaaaaattac
     3961 ttaatacagt aaattaattg gtaagataaa ttatttatta ttagtaaagt aaattattta
     4021 ctatttatca agataaatta tttataaaat aaatcattta taaaatataa tttatacttt
     4081 gaaaataaaa taaactatct tccaaacatt ttattatatg cttaaaagat agttttgtgt
     4141 ctttaaaata aattaaattg tatatattcc ttcacataac ttttgtatat tactaggtgg
     4201 ttgccattta ccatcttttt tagtatatgt aatcatagtt gtaacttcct tagttggttt
     4261 cttattgtaa aggtctataa tcatttttgt catatcatct tgaacttctt tttctttttt
     4321 atcgtaattc ttttcatcag ttacataaga ttttactaaa gtgttcgctt cactcattac
     4381 tttactagta tctaaagctt tacttacaac tttcaaagtt atcttatcct tactagaaga
     4441 ttccactgtt actgtctttt caacaccttg aattaattta acaattgttt cgtatatctt
     4501 ttcagcattt tctttaccat aaagaggtgt caattgagca atcattgaaa ccttgtcctt
     4561 gctgttataa gtttgaatct gtttatattc agcttctgtt aatgcatcag aaattccttt
     4621 ttcatcatac ttatacattt ctaatgcagc taaattagct tcaaaattcg actttacttt
     4681 ttcttctgca ttagaacacc ctatagttag aactaaaact agagccataa tcgtagacaa
     4741 tattaacttt tttaccattt tcatacaaac atcctccctc aattaaaata ataaaaaatt
     4801 tatttatcaa cattataaca taaatttgtc taactataga attattttga atattttacc
     4861 acttccagtc tattccaata ccgttttctt ttgctttatc gtatattctt tttgctgtta
     4921 cgacatcttg tacacctatc ccaacagttt taaatacaat tatctcatca tcatcttctc
     4981 ttccaggaat agttccattt ataacgtttc ctaaatcacc actaaaatca tctttactta
     5041 tcttaccttc ttttaatgga attaatatat ctcctgactc agataaaact gcatctgttg
     5101 attcaaaaaa tatcttagac gctctagtta aacagtttgg gcacaactct tgcatatctg
     5161 gcatatatga acctactgca cttattaaag ctcctttttt cactaagttt ccattaagta
     5221 ctggttgttt tgatggagta gctaaaacta tcacatctgc gttatctatg gcttcatcag
     5281 atgattcaac tgcaactatt ttagtattat atttacttaa ttttaagttc atttcttctg
     5341 caaatttttc tctactttcc ctgtttctac taaaaactct tacctcatcc aaatttctag
     5401 cttctatcat ggcttcaagt tgtggctcac cttgtcctcc tagacctatc aaagctccaa
     5461 tcttagaatc ttttttagcc aatacatcta tagctgctcc agaggctgct ccagtacgaa
     5521 tctgagttac ataagtacca tccaatactg cactaacttc tccagtttct ccatctaata
     5581 atagtactgt tgctggaatt actggtttgc ctttttgagc atttttagga aatacagata
     5641 caatttttat tcccccacag cctaattctt ctacgtatcc tggcataaat aacatactag
     5701 catcttgact tggaacagat atattggttc tcaaaggatt tacactcttt ccttcacaat
     5761 aaattctaaa agcttcctta tcagcttcaa ttgcatctct cattgtaaat acttttttta
     5821 tgtcgtcttt ttttaaaagt agcattttta tacccccaat tttaataagt ttctatgcta
     5881 aatattatag cataacattt aatttaatct tctatttcaa atcaaataaa tctacatact
     5941 ctggttttag catagaactt atcaacgctt tatttattga aaattctggt attcccgctg
     6001 catatggagt tatatcatat aaatcaaaat atacaactag attctcatct tgcaagtaaa
     6061 agttttgatt ctcttttata cctttaaaat tatatattcc gatattttct tcatcctttt
     6121 tttcaagttc agctatttgt cttttaattt cttcatctat aacttcttta tatttagtat
     6181 tatccttaaa taagtccatt aattctaaaa attttccagt tctcatatcc acattatatg
     6241 ctatatcttg ataaaatcca tgtgctccac cactataagt ataatatctc acttttatac
     6301 taagagcaga atcagtattc tttttaagtt caaagtctac atttgcaaca aacttatttt
     6361 tctcgtcagg attatcctta agatactgct ttgcttcttt atatgattta ttataaaatt
     6421 caaatatgtc attttttatc ttatcatttg tagacctttc aacaatttta tttgaatttg
     6481 ttattacagg catgtttata gtactttcta aatactcatc atttttcact attgttttac
     6541 ttcctattgt acttactgat aaagtttgtt tactatttgt attacccttt ggtaatgact
     6601 ggttactcat aacagtcact gcttccttat tactatttaa attaattgaa gcatctttcc
     6661 ttaaattatt atatttatag tcatgtttaa ttgccaaaac agccattata gtcaatgtta
     6721 aaacaaatac atatcttaaa actttagctt ttgtatcgac tttaaaaaat ttcatattat
     6781 cattttcctc ctaaatataa atctctctta ttatatttgg agtttctagg gttttttatc
     6841 catataattt aataaaagat taatttttat ttataaaaaa ttaatctttt tgttttaaaa
     6901 tattaatatg tcttatcaaa cagtttgaaa tttcaccaag aggtaattgt aacataacag
     6961 aacctctatc aaaagctacc attggcattc catatactat acaagaattt tcatcctgtc
     7021 caattgtaaa taaaccattt tctttcattt ttaaaagtcc atttgctcca tcataaccca
     7081 tacctgtaag tattattcct attgaatttt tacctgctac ttctgctact gaatcaaaca
     7141 aaacgtctac tgatggacag tgaccactta ttctttctcc tttttcacaa gttacataat
     7201 atccttgttt atccgtacac aatttcatct ggtaaccccc aggtgcaatt aacacatttc
     7261 cttgttcgac tctatcatta ttcttagctt ctcttacatt cataatacac tgcttgtcaa
     7321 gccgttgtgc atacatataa gtaaatatag ctggcatatg ttgaactaca acaatccctg
     7381 gtgttgattt tggtaggtct tttataattt caagtattgc ttctgtacca cctacagatg
     7441 cgcctatagc aataaccata ttattaaaac gttccacatt accgcctcca aaaaatcata
     7501 tatcaaaaat acctttttat cgtttatata tagcaggttt tacatatttg tatttagtac
     7561 tacttctgtt gattacttct gcatgtccta caaacaaaaa actatctttt ttggtagcat
     7621 tataaaattt atttataagt tcttctcttg tttttaaatc aaaataaatc ataacatttc
     7681 tacaaaaaat caaatcaaaa tgttgatatg agaaatcatt cattaaatta aatgacttaa
     7741 aaattacact ttttctaatc ttatcacaaa ttttaaatgt accatccttg ttgtctataa
     7801 aatacttctt cttccataac tcaggaacat tgttaacatt tgattctatg tatatacctc
     7861 ttttagcttt ttcaagtgag ttagttgaaa tatctgtggc tatgatttta atgttccaca
     7921 actctttatt atatccaaaa taatcatcca aagtcatagc tattgaatac gcttcttctc
     7981 cactagaaca tcctgcactc catatattta tgtttcttat tttattattt tcttcccaaa
     8041 aaggaagtat actattctgt ataaactcaa aatgttgagg ttctctcata aaaaatgtgt
     8101 ggttagtact caatctattt attaggttta tagcttcatt tcctgtctta tctttaaata
     8161 atagattcat atattgacta aaagaactaa gttttttctc tatcattgta ttgtataagc
     8221 gaccttcaat taacgctctt tttttactta aatcaatacc atattctttt tttacatggt
     8281 ttacaagaac tataaattct tcatctgtga gttttatcat gcaaaaactt cctcctgcta
     8341 atttatcaga actaactata tgctattaat caaaaacttt cgaatatcta aaattaatat
     8401 agaattatct tcaactttcg caatctcttt tatatcttta ttaatattag tgttgttaga
     8461 attagtcgtc tctagtaaat ttagattttc tatatgaata acatcaacaa ctttatctac
     8521 aataattcca acttgatact catcaacctt tgtaattatt atacaagttc tctcattata
     8581 atcagcttta ggaagattgt actttatatg catattaata actggtatta ttcttcctct
     8641 caaattaata actccactta tatatgttgg aagacatggt acatacgtta ttgattgaaa
     8701 tcttatggtt tcaatcacat tttctaattc tattccataa ataacatcat ttacataaaa
     8761 tgttaatact tgtttaataa tttcatcttt agccatactt taaatcccct aatatatttc
     8821 tataatgcta tctgcatcta taattaaact tatacttcca tccccaagta ttgtacatcc
     8881 cgataaaccc ttatttttta tattaaatcg gttgaaataa actggaaatg gtttcacaac
     8941 aacttgctga ttacctatta tttcatcaac aaaaatgcat actgctttat gctcattttc
     9001 aataattatc atattaccat gcatcagatt ggttactttt gtttgaatat tatatatttt
     9061 atgtaacctt ataatgggaa tacatgctcc atatattgta attaactctg tcttatttcc
     9121 ttctgtaatt atttgattta agtctgtaag tttaaatgtt cttcttatgg atgatattgg
     9181 taaagtaaat agtgaatctc caactttaaa cttcataccc tctactatag aaagagttaa
     9241 tggtattttt atagtaaagg ttgtcccttc atcctgttta ctttttactt caattgaacc
     9301 tccaactttt tcaatacttt tacgaacaat gtccatgcct actcctcttc cagaaaattc
     9361 agttatagat tcttttgtag aaaaacctgg agtcataatc aaagaattga cctcgtcatc
     9421 agtatactca ttaatttgat gtttaagaag accttgttcc ttagcttttg ccaaaatctt
     9481 atctctcata atgcctctac catcatctga aatacttatc acagcttctc cgcctagatt
     9541 ctcagcagat aaaactatct ttcccacttc ttctttacct ttcgctatac gctcctcaat
     9601 aggctctatt gcatggtcca tagcatttct tataaggtgc ataaaaggtt ctgctaaatt
     9661 atcaattaca ttcttatcta cctctgtctc tgctcctata gttattaact ctacattttt
     9721 atctaatttt ttattcatat ctcgtacaat tctatgcatt ttttgaaata caccagcaat
     9781 ttgaatcata cgcatagaca ttactatatc ttgcaattca tctgtaagct tgtgtaattg
     9841 tcttgttgcc ttattgaaat tatctatatc aacacctgta gaatttacgc tatttacaac
     9901 cattgattcc ataattacaa tttctccaac tatattttga agtaaatcca atttttctaa
     9961 atttacactc ataatactct gcttacttat tggtaaatgt tcattttctt cacttagact
    10021 atctttttcc atatcagttt tttcatttaa aacatcttct tgtatctcat cttctgtata
    10081 gttattttga taacttttac ttactttatt attatctttt aatttatttt ctactgcatc
    10141 attatttgat gtatttttca tttctatttc ttttttacta atatttatgt attcttttac
    10201 atagagatta tcttctatag ttttataaac tattgcctta tcctctttag ctacaaattg
    10261 tatataaaac ccataacttt taatatattc tgtagactct gcatttgttt ctacatcttt
    10321 tggataaaaa tctataaatt cacagtaatc tctaagttca aatacaagca tgaacgctct
    10381 cacattttcc atcatgcaac cttgttcaaa gattattttt actacatcag agtcttcttt
    10441 gtattcaaaa tttgaatcga ttgcttcttt ttcgtctgta ctttctaaat ttgcattatc
    10501 ctttgatttc atagaacttg ataattccaa agtactcaca tactcttcaa ttccttcaat
    10561 aaaattagag taatcagaag atatacaacc tcttgattgt agctcatcta tatctcttct
    10621 tataaaatct aatgattgaa agactaaatc aaataatagg tcattgtcta aaacttctat
    10681 tttaccttca cgtatagcat aaaacaagtc ttctacacta tgtgctaaag cagatatatt
    10741 gtctaacccc atcatagatg ctgaaccttt tatcgtatgc attattctaa aagtttcatt
    10801 aatgttgtta tgagtaaata ctttctcttt ttctatttga atcataattt catctaattg
    10861 ttcgagtaat aaacttgtct caaatataaa catatctaac atttgtctca tactcggttc
    10921 aatataattc acatttatca cctacgacct acaaataatt ttctattata tatgcatctc
    10981 ataatttaaa tccttgatga ttctcttatc tcactatatg tacctcataa tttaaataca
    11041 caatcattct cttatttcaa tatatataca tctcataatt taaatacatg ataattctct
    11101 tacttcaata ttcacctcat aatttaaata catgataatt ctcttacttc aatattcacc
    11161 tcataattta aacccatgat gattctctta cttcactttc ctttagatga aatttttcta
    11221 ctaattcttt taaaatttga gcttgagcag acatctcttc tgaggctgct gcactttcct
    11281 ctgctgttgc tgagttcatt tggactacta tggaaatttg ctctattcca gataaaatct
    11341 gtgtaattgc tacttcttga ctatgtacag ccgaagaaat accattaata taattagtag
    11401 ttttttctac accatttact accaagtcca atgcatttgc agtctcttta gcaattctga
    11461 caccattttc tactgtttta atagcttctt ctatgagaat agctgtattt tgagctgctt
    11521 ttgcactttt accagccaaa gacctaactt catctgctac aactgcaaat cctctaccat
    11581 attgacctgc tcttgctgct tcaacagctg catttaatgc gagaatatta gtttggaatg
    11641 ctatctcatc tattgttttg attatttttg atatttcact cgatttttca cttatttcac
    11701 ccattgatgt catcatatca tccatacatc tacttccaaa accaacttgt gatgcagatt
    11761 ggtcagatac ctcacatgca agactagcac tatctgcaca cttcttaatt ccatctgata
    11821 tctcattaat cgtggaagct aattcttcta ctgaactagc ttgttcagta gcaccctgtg
    11881 aaagagcttg tgcacctgct gcaacttgtt ctgaagcact tgcaacttgt tctgctgata
    11941 aatttatttc agaaaaatca ccattaaaag agtttattat actttctaaa gatattttaa
    12001 ttgatttaaa atcacctata aattcttggt ctatctctac tcttaaatct ccttttgcaa
    12061 tactagttaa tgtactgtct ataaggtcta tgtaatttaa aagcatactt tgcatctttt
    12121 tgacagaact cactaatagt cctatttcag atttactttc ttcaacgtct atacttgttc
    12181 ttaagtcccc agatgcaatt cttgaaaagt gtttttctac tctattaata ggaatcacta
    12241 tctttaattt tattactgta tattctatta ttgtcaatat tattaaaagg gaaagtataa
    12301 atattgttct taatattaga ctttgtaatt ctttttttac ttctacaatt ttggaatttg
    12361 aacgggtaac tatatcctct tgaaattcat ttattaaaga atccattttc tcttgggagt
    12421 ttatatagtc actaccaaaa attatacttt gagctttact cttatcatta ctttcgactg
    12481 cctttattgc actttgttct gttggtacta aagaatctga tatttctttt gcttctaaga
    12541 ttttatttga ctcgctcttt gtaagaccaa ttttttctaa tttgtttaaa gctgattctc
    12601 ttgtttttcc gtataattct ttttcataaa tatctttatt ctttttttct cctgtaaata
    12661 catatcttct taatacatca cttatctctc tagttgtatc ttttatatac tgactttgag
    12721 tcctcaaatt attcatatca ttaatacttt tttctacttt ttcaaatgct ttataagatg
    12781 aaatagaaga aaaaatccct gctagaacag ccaatactat aataatcagc aatacaattg
    12841 ctaatttaga aagctttact tctcttttca tatctgtact ccttcataaa gtaagtttat
    12901 atctttttta atcctttcat tgtactcact acgctacaat cttttcctaa gtttttaccc
    12961 tcatacattg ctttatctgc taaggatatg ttttcttcta atgatatatt tgagtttact
    13021 tctactactc ctaatgtaat agtgcacttt attttatgtt ctttatagtt gaaatcaaac
    13081 tcctttatct ctttacggat tctttctgaa agtatcttag caccttctat tggagtattt
    13141 gtgagcatta tcaaaaactc ttctccaccc catcttgata cagtatcaat ttgtctacaa
    13201 ttctttttta ttatacttga tattactgtt aacacgaaat cccctgcttc atgcccataa
    13261 gtatcattga taaccttaaa gtcatcaata tcaccaagga taagggatat tgatgtttca
    13321 ctatatttat taattcttct ttgctccata agctgattaa ttatatgtcg cctattgtaa
    13381 agacctgtaa gataatctct tacaacgagt ctattcaatt tttcaagaga agattccagt
    13441 tcattattta tcttttttaa tttatcttga gatttcttta cttcaagatg agctataaca
    13501 cgagctttta attctgtcat actaaaaggt ttcaccatat agtcaattgc tcctacacca
    13561 aatcctttta ctatgtctgt atctgtattt tttgaagtta taaaaattat aggtatatct
    13621 gctgtacgag tattagattt tacttcctta caaaactcaa aaccagagtt atctggtaaa
    13681 attatatcaa gcaaaataat gtctggtata aattcttcaa taaattcttt tgcctctatt
    13741 gcattaaatg cttttttaac cttaaaatta tcttttgtaa gtgttttatc tattaagtca
    13801 cacatcagaa cactatcatc aacgactaaa attgttccct gttgcttatt tttcatatac
    13861 aaacctctct taaacataat atattcaaat tttaatgatt taaaattttt atacttaata
    13921 attataccac tatattctta aatgttctca tcacaatctt tttataataa cactccaact
    13981 acatccctat gttacatgat agtgcaatca aacccattga ttaattcatc tattaaaact
    14041 tcatctttat ctgtcaaaag tttctctaaa tctagtaaag taattatttt accttctaat
    14101 tctccaacgc cacttacaaa ataatccgaa tattctatag taactcttgg tggaggtgat
    14161 attttacttt tttctaagct tactatatca tcaactctat caaccacaaa tccaactacc
    14221 aagtccttta tttcagttac tactatatac atgcaatttg tagtcattaa ttcatctttt
    14281 ttaagtctca aacgcatatc tattattgga atgatttttc ctcttagatt tattatgcct
    14341 ttagaatatg taggtagttc tggaataaat ataatttctt gatttccagt aatttctatt
    14401 accttttcaa gttcaacccc aaaatcttgc tcatctactt taaactttag atacttaatt
    14461 ccttcatcaa ttagtccata atctaaatat gggtcatctg gaaatctcat ttaatacctc
    14521 cctcctaaaa tacactttct atgaaatata tattataatt tcttcttacc tttatttatt
    14581 gaatctacaa gtaattcacc cgtctcagtg taaaaacgta ttgttctacc ataattcata
    14641 cctgtgtcct cagctaatat tggaatattt aatcctaaaa gagttttttt tactgcctca
    14701 acattcctat gtcctatact tgcaattact gaattgctgc ttcctaaaaa catctgagca
    14761 cctccagcaa tttttgcttt tatatatatt ctatttgcac ctatattctc catagattta
    14821 atgagctcat ttattcctgt gtcagcaaat ttacacttat tttcaatgtt aatagcatct
    14881 ttactatatg gcaacataac gtgtaacatt ccaccaattc cagctacttt atctatcaaa
    14941 catactccta cacaagagcc aagtgcataa gtaactaaaa catttggtct ataggctatc
    15001 ttcatatctg caatgttcac tactatctct ctattcatac ttctaatccc aacctcacga
    15061 gtatttctct aagtgagctc atttctggaa tcatcaaaat gtggctagta aaataattat
    15121 cactcatttt aaatttattt tctataaata agactttgtc accaacatct gaaaatctta
    15181 tcataggcac atttagaaca gctccaacca tatcaataca tatatctgga attgaaacac
    15241 ttatagtcat gtttaacata gatgcaatag catttacata tgaacttgcc ataatatttc
    15301 ctatttcctt aattgctgaa atttcaattg cctcaatctc atctaaaaaa gcttcttctt
    15361 tattaaaaag agaacttagc ataagttgag ttattgattc atccaacaaa aacataaaca
    15421 ttccattgat atcacctttc atatcgagta aaatacatac tactttgttt tctggaccac
    15481 caagcaaatt tgtcgcttca ttatattcaa gtatcttgac cataggtatt aatacttcta
    15541 cattgctatt tatcatggat gcaagtgcag taattgcatt cccagagcct atattaccaa
    15601 tttctctaag agcatctata tgaacatcat caagttctaa ataatttata atataaaccc
    15661 ctccctttta ttattgcatt tatttgcttt tcattgtatc agtgtatatt tacactttta
    15721 ttttaaaaca ttatttacaa ctttaactaa tgtttctgtt ttgaagggct tcactataaa
    15781 atctaacgct ccaagtttta tggcttctac aatcatattc tcttgtccta ttgcagagca
    15841 cattacaacc ttagcacttg aatccaactt ttttatttct cttagcacct ctaaaccatc
    15901 tttttttggc attgtaatat caagcaacat caaatcaggc ttttcatatt taaatatatt
    15961 acacgcttct tctccatcat ttgcttctat taaattatta tagccacttt tactcagagc
    16021 ttctttaatt atcattctca taaaaatagc atcatctact atcaaaattt tattttccat
    16081 aaaactaact cctccatatg tacaacaata aacgctttta tcttcagtta ttgtctatac
    16141 tatctgacta tttttcgcta cacaataaat acatttcatt gataaataaa agtaattatt
    16201 tatgccatat tatacttaat tctcttaaca taatttgtca acaaaaaaac ttatatatct
    16261 attgtaatat atgtttggcg ttttgacgag ttattaaaaa tataattgta taaaacataa
    16321 aaagttatca aaagtaatca aattataata aaacaacttt ataccttttt tatgtttgta
    16381 taaaaaattt tttcagattc attaaaatca tttctgtaga ttcatatagg atttgacttt
    16441 ctcaatatct aatagatagt tgtttattta cttaattata tttctctata tgtattatcg
    16501 attaaattag atttagcttt atacattatt ttttatattt ttcaaattgt atactcataa
    16561 atccagaagt attctaatgt gtttataata ctttttttca tattttaaat taaataacaa
    16621 atcattatat ttacaagtat catttatcaa taaacaatat taaatgacga atatgtctga
    16681 agattcaaag tgatactgat actattaatt actaaaaaag cagtaaatct tttacagatt
    16741 tactgcttat agttataaat agtaaaacta tctataataa atgtactata ctcaatactt
    16801 cattcaatat agtaataaca aatctacttt attcctatgt aaatatgaat ttcttgtttc
    16861 tttgtatcaa aagtattaca ttgatactct tcaaaatcac ttatataagt cctatcaaaa
    16921 tccatttgcc aaaacttaaa ccaaaaatct cctacttctt tttgacctcc tactataaca
    16981 aactttgcat acttacctgc tagtataact ttactaataa tactttctcc aacagtatta
    17041 ttagtgttta attcttctaa attcttctct ttatcagaat tactatttac ttcacaacat
    17101 gcaacaaagc tatacggact tgtaaaatca ccctggtaat ctgtatatag acctattgtc
    17161 ttgtcattct ttttaccctt tatagcatta tatatccctt tgcccataaa ttttttccac
    17221 aattcaccta tagcatttac cgcttgtcca tctttatttg tagtctcctt cgatattcct
    17281 ataactattt tctcatttat ctctactatt tcataattca tactttatac ctcactttgt
    17341 ttttattaat atattttatt ttgaactaat tcaattataa tttataatat acgacacctt
    17401 tatgtcatat ttttaatata attaaactca gtgagatatt atatactaaa tgcaagatat
    17461 tatatattta ataaaatctt taatctagca tcatattata atgaataaat tttagacaac
    17521 tcccttagtc tagttattac attatcccta acatctttag gttcaagtac ttcaacattg
    17581 tcaccaaacg ataaaatata tccataaatc cattcaccaa taggaaattc tgtaattaca
    17641 tcatatgtat catcttcatt tttagttatt aaatccttat taaaatcatc aaatactcta
    17701 aaaagcatct tttctttaaa cctcaatttc aacgttatag tattttcctt aattacttta
    17761 gaatcattca aacatacttc ttctattatc tttcttgaaa atacttcatc ctttatagat
    17821 aaattcctta tcctactaat tctaaatatc ctaaaatcat cttttagttt acaaaatcca
    17881 tacaagtacc aagtcttact cttatacata agcttcagtg gttcgacagt tctgtttgtc
    17941 tggcttgaca aactatttac ataattaaaa tttatagtat tacgtcttaa aatagcttct
    18001 tttatttttg taaatttatc atcttcatta aaattacttc cccattcagt aaagtctatt
    18061 tcaatccagt ttgaaaaatt ctgctctcca aatattgagc caattttatt tactactgaa
    18121 tttatttctg gatacttagt cgcctgtaat gttttcagtg caactattaa actctcttta
    18181 tctttttttg acagtatggc cttgtctata gaatattctt ctattaaaga tatccctcct
    18241 cctttgcctt tcgtcatata tactggtact cccgacatag acagagtttc tatatctcta
    18301 tatatggttc ttgtagaaac ttcaaaccta tctgccaact ctttagctgt tactaatttt
    18361 ttatttaata gaattactat tatttctgtt aatctattta ttttcattca ttaccaccta
    18421 ctaaaattat taaatttatt taatctaaat taatcatact aatttttaaa taaataaaat
    18481 cttagaatct tatctaaatt attcttacat gattttattt actttctatt atctttctca
    18541 ctttggatat tatcatctta atttatttat tactatatca atttacttat tatcatctca
    18601 tttgttatca tcttacttaa tttacttatt tctatatcaa ttcacttatt atcatctcat
    18661 ttgttatcgt cttacttaat ttacttattg ctatatcaat tcacttatta tcatttcatt
    18721 tgttatcgtc ttacttaatt tacttattgc tatatcaatt cacttattat catttcattt
    18781 gttatcatct tacttaattt acttatttct atatcaattc acttattatc atcttaattc
    18841 atttgcttat tatcatttac tttattcaat ttctatatca aaaattagta tttgtactta
    18901 atacttaaaa ttaatttaaa gtctttacat attatttatt ttctattgta atattcattt
    18961 ccatacttta ctaagctact tccactcatt tattttaaaa tttcaattta ctatttctca
    19021 actctatatt attatttttc aattttatat tactaatttt aaagcttttt agctattatt
    19081 cctacaatat ttaaacaatt ttttcataat aaagctatat tacaatatat tcaaaatatg
    19141 taatatttta gatatttttc gttgacaatg ttaaattttt gttttatcct taattaagga
    19201 catgttattg acatttctga aacttatttg aaatagtttt ttcattttat ttaggaaaac
    19261 gtattatact taagaaaacg tattttctca tttcagataa gtaaaatatt tattcttttg
    19321 gggaggaaat catggaaatt aataaaaaac attttattca agtattttta acttgcttaa
    19381 tcttggtagt aatatgtgaa tttatagggc aaagaactat agctattgga aaagcctcta
    19441 tagtactgtt ccctatgctt tatgcagtta taattggact tataataaca ccagatttat
    19501 taggtaaaaa aattaaagca cttaaaaaag tggttggaga aaaagaaata aatattgctg
    19561 gtgaagttgt tggaattgca ctagtaatgc ttggtataaa atatggtact acagttggac
    19621 caaacattga caaaataatt caagctggac cagcatttat cgctcaagaa tttggtcata
    19681 tacttgcacc aatcgttgca ttacctcttg cattaatgtt tggaatgaaa agagaagctg
    19741 ttggagctac tgcaagtata agtcgtgaac cttctttagg agttatatca gaaaaatatg
    19801 ggataaactc accagaaggt agtggtgtac ttggtacata tttaatggga acagttcttg
    19861 gtactatata ctttagtgta ctaggttcaa tatctattta tagtggtctt cacccatatg
    19921 ctttgggaat ggcctgtggt gttggtagtg gaagtatgat gacagcagct gctggttcat
    19981 tatctaccat ggtaccacct gaaatggcag atacaatact tgcctatgct gcaacaagca
    20041 atatgatgtc tagtataaca ggaattatat tcttagtttt tgtatcctta ccatttacta
    20101 attttatgta taagatttta gaacctaaat tttcgagaaa taaaaaaact aaagaaatta
    20161 aatctacaga aggagagtgc taataaatgt ttgctaattt aaatttaaag aaaagttcat
    20221 atcaattatt ggttatgtct gttattggag ttataattct tataggacaa agaattagcg
    20281 ttggtacatc tatagttaca gctcttcctg gtatggtgat gctaatatta gctgctatgg
    20341 cagctatgat tataaaagat ttatttccta aatcaatctt tccagctttt ggatttgcaa
    20401 ctataatcgg ccttttactt agtatgccat atagtccaac atctgaagta ttcttaacta
    20461 acactaataa tattaacttt atggctataa ctactccact actagctttt gcaggaattt
    20521 ctgttggaaa caaaatagaa gctttaaaag aaatgtcatg gaaaatagtt ataatttcac
    20581 ttattgtatt tacaactata ttctttgcat gtgcgagtat agctcatata gttttaagta
    20641 ttcaaggtaa aatataggag gaatttatta tgaataaaga acaattaaaa tctctagtaa
    20701 taaaaactat agatgaaaat agagaaaaaa tattaaaaat aggtcaagga atatacaaaa
    20761 atccagaata tggatataaa gaatttaaaa ctacagaagc tgtttcaaac ttttttgagc
    20821 aagaattaaa tttatctgta gaaaaaaata tagcagttac aggatgcaaa gcagatgcaa
    20881 attctagcaa gaaaggtcct catatatcta tacttggaga attggatggt atatcatgta
    20941 aagaacataa agatgctaat gaaataggtg cttctcatac ttgtggacat aatattcaaa
    21001 tagctggaat gttaggtgct gctgttggtt tagtaaaaag tggcgtttta gaacatttag
    21061 atggtaaagt atcttttatg gctactcctg ctgaagagtt tattgagttg gaatacagag
    21121 agcaattaaa aaaagaagga aaaataactt attttggtgg aaaacaagaa ttaataaaaa
    21181 gaggttattt tgatgatata gacatatcta tgatgtttca tgcttctgat ttaggagaag
    21241 ataaggctct agttggtcct gagagtaatg gatttgttgg aaaaaaggtt aaatttatag
    21301 gtaaagaatc tcatgctggt tctgctccac atgatggaat aaatgcttta aatgcagcaa
    21361 tgcttgctat aaacaacgtg aatgcactta gagaaacttt taaagagtct gaaagagtta
    21421 gattccaccc tatcatcaca aaaggtggag atatagtaaa tgttgttcct gctgatgttc
    21481 atatggaatc atatgttaga gctagaacta taaatggtat gattgatgct aatgaaagaa
    21541 taaataaggc tttgatggct ggtggaatgg cagttggagc tgatgttgag ataactgaaa
    21601 ttcctggata tcttccaata ttacgataca aaagtcttga taatttattt aaaaacaatc
    21661 ttgaagattt aggtctaaaa ggaaaagtta ttgatggtgg agattttaca ggctcttttg
    21721 attttggaga tgtgtctcat ataatgccta ctcttcaccc tatgattggt ggtgtaaaag
    21781 gagctcttca tacaagagat tttgaaatag ttgatgaaga tttagcttac ataattcctg
    21841 ctaaagcaat ggcacttaca gttgtagacc ttctttttga taatgctaag gatgctaaag
    21901 atatattaga tacctttact ccagttatga ctaaagaaga atatctaaca ttcctagaaa
    21961 aacacgataa aactttataa gtctattttt ataatagggt ggtatctact gtaaaagtta
    22021 taccactctt tgtttttgta acagtatact tcttttcttt tagcttttag attaaactaa
    22081 aatatattat cctccttaaa acttttagag atacgttaaa atatcagtag aactatgcta
    22141 tattctttct aattaatata aatttatatt ctaattcaag gtggtgaaaa aatgtttaat
    22201 gcaggaaatc gaattataaa tacatggttg taccctattc aagatggctt tgtcttgatt
    22261 gatactggat atgaaaatgg ttttaaacat ttcaaaaaac gaatttctaa attcaacata
    22321 aaaatcaaag atattcgata catatttcta actcatgctc atgatgacca tgttggtttt
    22381 ctaaatcaga ttctagacga atctcctaat acaaagattg ttatgcatga taaagcttta
    22441 gaaattctat ataaaggtca aaactctttt attggtggtt gtaacagccg tactgccttt
    22501 ctcttttgcc aaattatgaa actatttgga aaaggtgagc atagtttttc tccacttaaa
    22561 caagaatttg aaaaaaggtg tattcttgtg tctgacaaga atcgtaaaga aattgaaaaa
    22621 gaactaaatg gaaaaattat agatacacca ggacatactg ctgattcaat ttcactacta
    22681 attgatgatg gtgttttatt ttgtggagat tctgcaatga atggatttcc aagtactcac
    22741 aaaattacca tatttgcaga aagcaaacta gaatttattc aatcttggaa aacaatcatc
    22801 aatacaaaac ctaaaatgat ttatcctgga catggaaaac catttgaata cagtaaatta
    22861 gaacaaaatc taatctatgt aaataaaata aatttatatc ctctacataa ttcaaaagat
    22921 attcacagat ttttgtagaa aatacaaagt aaatttcttt gattaattaa aaaatctaac
    22981 actttactac gctacttatt ttaataactt aatcttccac tagcttttca taaaattttg
    23041 ttttaaaatt aactttaaag tgcatatgct atttaaatac attagatata attcgtcatt
    23101 aatctactta aagcatattt aactaacacc actcaaccag aaaattcaga aaactgcatt
    23161 gacacaggaa aaagtttatt ttatactttt tttaggacat attgtggaca cgtcttaaat
    23221 cgacaagcag aatttatatt gtaagatcaa ccacaatact tattttttaa ttatattttt
    23281 aaacattagg gaggatatta tgaatgataa cgtagttaag aagaaaaact tttttgacag
    23341 gacattaaac agaatagaaa ctgttggtaa taaactacca gacccagtta caatattttt
    23401 agggttatgt gtacttttac ttatactatc tagtctagtt ggttctatgg ggatatcagt
    23461 agttcatcct ggtactggag aaactataac tgcagtaaat ttattgactg tagaacagct
    23521 tcaaattctt ttaggaaaca tagttagcaa tttccaaggg tttgctcctt tgggtctagt
    23581 tctagtaact atgataggtg ctggtgtatg tgacaaaaca ggacttatga cagcaacaat
    23641 caaagcatca gtaagcaaga ttcctgaaac aagagtaact cttgttgtaa tgactatagg
    23701 tatgcttgcc aatatagctt cagatgcagg tacaatatta tttccaccat tagctgcact
    23761 tgtatatctt ggtgttggta gacatccact tataggttta ttttctggat atgcagcagt
    23821 ctgtctagga tttgccgcaa acattatgat aagtgtcaat gatatacttg ctgcaagttt
    23881 tacagttcca gcagctcaaa tgattgattc tggatttcaa gccaatgcaa caatgaactt
    23941 attttttatg atagcatcaa cttttgttct aatacttttg gcaacatggg taacagaaaa
    24001 aattgtagct cctagatttg gtaaatatga aggaaatgca gaactagatg ttgatggaag
    24061 tcttagtgat gtagaaaaga aagggcttaa aaaagctggt ataagtatat taatatatgt
    24121 agctattata gtagctctta gtgtaatcgg taaaaaacca ttcttagctg acccagaaac
    24181 aggagattta ctatcaaaca atgctcctct tatgaaaggt atggtaccta taatagcact
    24241 agcatttttc atacctggtg ttgtatacgg aaaaactata ggtaaaataa agaaggataa
    24301 ggatgtagtc tctataatgg ctagtgcaat gagtgatatg ggtggatata taatactagc
    24361 atttgctgct tcacaatttt tgcaattatt cacaaatagt aatttaggtc tgatactagc
    24421 tgtaaagggc ggagaatttt taaaatcagc aggaataaat ggacctttat tgttgatagg
    24481 atttattata ttatcatgtt ttataaattt atttataggt agtgcatctg caaagtgggc
    24541 tattcttgca cctatatttg taccaatgtt tatgatggtt ggatacaatc cagcacttac
    24601 tcagatggct tatcgtatag gtgattgtat aacaaatcca cttagcccat tatttccgta
    24661 cttccctatt atactagcat tcacaagaaa atacgataaa gatgctggta tgggtactgt
    24721 aattgctaat atgataccat actctatatc attcttaata gtgtggacta tactattaat
    24781 attgtttatg gtatttaata ttccactagg acctggaata cttccttcat atagcatgta
    24841 atttatttaa atctttctaa aaggagaaat aatttatgtt agataaaaat agacttataa
    24901 ataattttat ggatatggtt agaattgata gcccatctaa tcaagaatta gaaatgtcta
    24961 agtggttggt aaactactta aaagaaagaa atatagatgc cattatagat gatgcaggtg
    25021 aaaaatatgg tggtaataca ggtaatgtca ttgcctatat aaaaggtgaa gaaggaagta
    25081 gaccactttg tttatgtgca cacatggacc aagttcaacc ttgtttaggt gtaaaaccaa
    25141 tactagatgg taatgtagtt agaagtgatg gaactactac acttggtgca gatgataaag
    25201 ctggaatagc tgctatactt gaagctttag aacatgttac tacagaaaaa ataccacata
    25261 gagatattta tctttgtttt acaatctgtg aggaagctgg tatgcatggt gtcaaaaact
    25321 ttaatcctga caacttgcct tgtaaagata tggtgatttt agattctggt ggagctattg
    25381 gttcgatagc atacaaagct cctgctcaac aatctataaa aatctctttt catggaaaga
    25441 aagcacatgc tggtatagaa cctgaaaaag ggttaaatgc tatattagta gcctctcatg
    25501 caataagcaa tatgcacata ggcagaatag atccattgac tacttcaaat ataggtaaaa
    25561 tagaaggtgg tggagctaca aacatagtaa ctgataaggt tacattgact gctgaaatta
    25621 ggtctcatat acctgaaact ttagagtatg agcttaatca tatggaaaaa tgttgtaagg
    25681 atgctgcatc taaatttaat actacttata cctttgaaca tagtatgtct taccctagtt
    25741 ttgaacttag tagagatagt cacgttttca agttaagtga agaagcaata agacaagttg
    25801 gtgttgttcc aaatccaatg gtaattggtg gtggaagtga cgcaaatata ttagctaatc
    25861 ttggatataa ttgtgctata ttaagtctag gtatgtatga tgtacacaca gttaatgaat
    25921 atgtaaatat agacgaatta tatgatactg ccaaaatagt ttatcatatg attaaa
//
LOCUS       DAGQWR010000051        25950 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.051, whole genome shotgun sequence.
ACCESSION   DAGQWR010000051 DAGQWR010000000
VERSION     DAGQWR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25950
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.051"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            176..1393
                     /locus_tag="MIW95_001797"
     CDS             176..1393
                     /locus_tag="MIW95_001797"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBY3151125.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1488..1868
                     /locus_tag="MIW95_001798"
     CDS             1488..1868
                     /locus_tag="MIW95_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBY3151126.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2152..2634
                     /locus_tag="MIW95_001799"
     CDS             2152..2634
                     /locus_tag="MIW95_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY3151127.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVNLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2655..3470
                     /locus_tag="MIW95_001800"
     CDS             2655..3470
                     /locus_tag="MIW95_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY3151128.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3521..5071
                     /locus_tag="MIW95_001801"
     CDS             3521..5071
                     /locus_tag="MIW95_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY3151129.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5168..6520
                     /locus_tag="MIW95_001802"
     CDS             5168..6520
                     /locus_tag="MIW95_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBY3151130.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAESLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKVFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6651..7409
                     /locus_tag="MIW95_001803"
     CDS             6651..7409
                     /locus_tag="MIW95_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3151131.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIYNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7456..8175
                     /locus_tag="MIW95_001804"
     CDS             7456..8175
                     /locus_tag="MIW95_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBY3151132.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8299..9699)
                     /locus_tag="MIW95_001805"
     CDS             complement(8299..9699)
                     /locus_tag="MIW95_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBY3151133.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9703..10611)
                     /locus_tag="MIW95_001806"
     CDS             complement(9703..10611)
                     /locus_tag="MIW95_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY3151134.1"
                     /translation="MKGDNKVTEKQRLIINIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10872..11519
                     /locus_tag="MIW95_001807"
     CDS             10872..11519
                     /locus_tag="MIW95_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBY3151135.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11844..14483
                     /locus_tag="MIW95_001808"
     CDS             11844..14483
                     /locus_tag="MIW95_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBY3151136.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14541..15644)
                     /locus_tag="MIW95_001809"
     CDS             complement(14541..15644)
                     /locus_tag="MIW95_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBY3151137.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSSK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16005..16826
                     /locus_tag="MIW95_001810"
     CDS             16005..16826
                     /locus_tag="MIW95_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBY3151138.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16905..17116
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17203..18519
                     /gene="argH"
                     /locus_tag="MIW95_001811"
     CDS             17203..18519
                     /gene="argH"
                     /locus_tag="MIW95_001811"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBY3151139.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18768..19106
                     /locus_tag="MIW95_001812"
     CDS             18768..19106
                     /locus_tag="MIW95_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBY3151140.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19233..20522)
                     /locus_tag="MIW95_001813"
     CDS             complement(19233..20522)
                     /locus_tag="MIW95_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBY3151141.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20602..21306
                     /locus_tag="MIW95_001814"
     CDS             20602..21306
                     /locus_tag="MIW95_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HBY3151142.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21398..22444
                     /gene="selD"
                     /locus_tag="MIW95_001815"
     CDS             21398..22444
                     /gene="selD"
                     /locus_tag="MIW95_001815"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:21446..21448,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBY3151143.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDNPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22462..23874
                     /locus_tag="MIW95_001816"
     CDS             22462..23874
                     /locus_tag="MIW95_001816"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBY3151144.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVAELRKAFN"
     gene            23988..25871
                     /gene="selB"
                     /locus_tag="MIW95_001817"
     CDS             23988..25871
                     /gene="selB"
                     /locus_tag="MIW95_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBY3151145.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALEALISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
BASE COUNT        10068 a         2956 c         4589 g         8337 t
ORIGIN      
        1 taatttaaat gaattgaaga tatatgttta ttgtatgcaa ttgttgtatt tttaggtttt
       61 ttgtgattaa ttaaaaaatt ataaaaatcg tttacatgtt gatttttaaa aaaaaaagat
      121 atagaattat atagaaagta ataaacaaaa attggattta cgggagtgga atgaaatgat
      181 gaatgttacc ttaacagatg tgaaagaggc tagagagacc attaagaaca tagtaaaaag
      241 aactgactta cttgaaagtg ttaaacttag cgaaaaaaca ggagcaaatg tgttctataa
      301 atgtgagaat cttcaaaaaa caggttcttt taaattaaga ggagcttgta acaaaatagc
      361 gagtttaaca gatgaagaaa aggcaaatgg agttatagct tcaagtgcag ggaatcatgc
      421 tcaaggagtt gcattgggag caaaaatgac tggtataaaa tctacaatag taatgccagc
      481 aactgcgcct ttagcaaaag ttagtgcaac taaaggttat ggagcagaag tagttttaaa
      541 tggtgcggtt tatgatgatg catatgcaaa agctgttgaa atacaaaaag aaacaggagc
      601 aactttctta catccattca atgataagta tgttatagca ggacaaggaa ctatatctct
      661 tgaaattttc gaacaattag acaataaagt tgatactata ctatgcccag ttggtggagg
      721 aggaatcatc tctggtgttg ctgttgcagc aaaagcatta aatcctaatg taaaaatagt
      781 cggtgttcaa actgcaaata taccttcaat gaaagagtca ataaaaaatg gaaaagtaac
      841 aacagcattt aatgatacta caatagctga tggtatagct gttaaaactc caggagattt
      901 aacttttgaa ataataaatg aattggttga tgaaatagta gtggttgaag agacagaaat
      961 tgcagaatca atattattta tgatggaaag tcaaaaaata gtttcagaag gtgctggtgc
     1021 tgtatgtaca gctgctatat taagtggtaa gtatgtacca gcaaaagatg aaaatgtagt
     1081 ttgtataata tctggtggta atatagacat aaatacgtta tatagaataa taggtgttgc
     1141 tttagctaaa gaaggaagaa gatattcatt tagtacaatt atggaagata agccaggaaa
     1201 ctttgctgag ttaactagaa taataagtga gaatggtgga aacattttaa gtgctaatca
     1261 aggtaaatta tcagcagggg aagctctagg aaaacaaagt gcggaattta tattagagac
     1321 aatagattat gaccatatag ctagaataaa gaaagctata gaagaaaaag gttttaaaat
     1381 tatagaatta taataaaaag tgtatataat atacaagaaa aatagtataa tagtgtatga
     1441 agatacattt tatgtgtatt agattaacga attaggagga ttttaaaatg aaacatgaag
     1501 taatacatac taatgatgca ccagcagctt taggaccata ttctcaagct ataaaagctg
     1561 gaaacttatt atttgtatct ggacaagttc ctcttgtacc agagactatg gaagtagttg
     1621 aaggagatgt acaagctcaa actgctcaat ctttaaaaaa tcttaaagct atattagctg
     1681 aaagtggagc tgatttttca aatgtagtta agactacagt atttataaaa gatatgaatg
     1741 aatttggggc tataaacgaa gtttatgctg aatattttgg agaaaataaa ccagcaagag
     1801 cttgtgtaga agttgctaga ttaccaaaag acgttaaagt tgaaatagaa gtaatagctg
     1861 tattataaaa atacaatatt tttaatttga aaaggctgaa aaactatatt ctagtgattc
     1921 agtcttttta taaaaaaata aatcacaata aagaacatga taaatattgt cgaattaaaa
     1981 aataaaatta aaataaatat tgcaatattt ttgagaaggt tttataatga gtatataatt
     2041 tgaaacgtgt aactattaaa ttaggcatga gttcttggat atttttatcc tgaggattca
     2101 tgcctttttt gtattgaaat tcaaaaatct atttaataag gaggaagtat tgtgttaaat
     2161 tttttcaaga aaaataaaag ctataagtta catgcagtag tgagtggtaa cagcattaat
     2221 attgagaaag taaacgattc tgtgttttca aaaaagttaa tgggagatgg agttgcaatt
     2281 attcctaata gtaatgttgt tgtagcccct tgtaatggta aagtaactgt gcttacagaa
     2341 tcaaagcatg cctttggtat ggtctcagat gagggtgttg aaatattagt ccacatagga
     2401 attgatacag ttaatttaca gggtgagggg tttaaaaatg aagtgtctca aggagatact
     2461 gttaaaaaag gtagtccaat aataagtttt gaaagagaaa aaataaattc tcagggaatt
     2521 gattgtacaa caataataat tgtattaaat catagcgaat tttcagaaat aaattgcatg
     2581 gtagaaaatg aagttgttgc agggcaagat actgttattg aaattatgaa ataataggaa
     2641 gggattaatt caatatgaga gtagtaaaaa tctttaataa taatgcatta tctacagtaa
     2701 ctaatgatag gaaagaagct attttactag gtcttggtat aggttttaat aaacgtccag
     2761 gagataaagt taatgaagat aaaatagaaa agatttatta tgttcaagac cacatgcaaa
     2821 caaaattttt agaattatta aaaaatgtta gtccagaagt gatggatgct tcacaacaaa
     2881 ttataagtct tataccagga aatgagggaa aatttaataa taaagggata ttatctttag
     2941 tggaacatat tagttttgca atagatagaa tgaaaaataa tgttttttta cctaatttaa
     3001 tgcttttgga tataaagatg atgtattcaa aagagtttga attaggagta aaagctttag
     3061 aaattatata tagagtatgt catatccatt tgcctgaaga tgaagcaggt tacatagctt
     3121 tacattttgt caatttacaa tcaaatgata atttggcata tgatacttta aaatttgtaa
     3181 aaggtagtat tgatttgatt aaagaatgtt atggattaga attagacgaa tcgagtttaa
     3241 gtacattacg ttttagaact catcttaaat ttcttgctca aagaattttt aagaatgaaa
     3301 tttgtcagga tgataaaatg attgaaatgt atgattattt aattaacaat catcctaaaa
     3361 ataaagaata tttagaaaag ttaaatgtat atattgaaaa ggagtttagg tataaattag
     3421 ataaacctga aaaaatttat ctactactac acctaacaaa aatcttataa gaatgagatg
     3481 aattagtaat agaaatatat aattttaggg ggggataaga ttgaaagaaa aactacttgg
     3541 aggactggaa acatttgcaa aggcaatggt tcagccatta atgtatttat cgagtgcagg
     3601 aatattaatg gtggtaggtg tactacttac aaatgatatg ttattaggag tacttccatt
     3661 ttttaaatgg gagccaattc aaatatttgg aagcctaatt tataactgta taatggctat
     3721 tattaataat cttgctgtta tatttgctat aggtattcct gctgcactag caaaaaaaga
     3781 gaagcacaaa gcagcgttaa taggatttat gagttatata atatacctta cagcttccaa
     3841 cactatgtta acatcactag gtcaactagc aaaagaaagc aagatgcttg gactgatagg
     3901 gactggacaa gctactattt taggtattca agttgtagat acaagtgtat ttggtggaat
     3961 tattttaggg tgtatagctg gatatgtatt taatcgtaca agcaacaaaa catttaaagg
     4021 agctttacag atatactcgg gagcaaattt tagttttata tgcatgttct ttgtatccat
     4081 tttaattgga gttgtgagta actttatatg gccattagct caacaaggaa tagcttctct
     4141 tgcaaatgtc atcaaagata caggtacatt tggtttattc ttatatggtt ttttagagag
     4201 aatattagta ccaactggac ttcatcattt aatctataca ccatttcagt tttctgattt
     4261 aggaggagtg cttaaagttg gtgcagatac agtttcagga gcctatgcta tagttatgac
     4321 agaaatgaat ttaccagttg agcgtttctc agatagtata tactacatgg caactggatt
     4381 tacaaagatg tttggttata taggtatagg agctgccttt atttatactg catataagca
     4441 aaataagaag aaagttaagg caacgattat tccactaata attacggcag tattagcctc
     4501 tgtaacagaa ccattagact ttatgtttgt attctgtgca ccacttttat tctttgtaca
     4561 tgcagttata gctggtcttt ttatagcttt attaaaaata tttgatgtta ctgcattatg
     4621 tggagggaac ttattagtat caattataat gaatactaca gtaggtgtag aaaaaacaca
     4681 ctggccagta atgatgatat taggacttgc acaaattatt gtatattttg tagtatttag
     4741 ttttttaatt aagaaattta attataaaac tccaggtcgt gaagatgaga gtacagctac
     4801 aggcgaagaa caatcaaaac aacttaattt agatgcaggt atagaaaata taattgatgg
     4861 gttaggcgga aaagaaaata ttaatacagt agaaaattgc atcactagat tacgtgttaa
     4921 tgtaaaagat gaaagtaaga ttaatgaaga tataattaat ttaacgccaa atagcgggat
     4981 tgtaaggaaa ggaaaagata tacaaataat ttttggactt catgtgcatg aggtaagacg
     5041 tgcagtagaa gattttttag aagcttatta aagatatata tttatatagc aagggtaaat
     5101 aacagcttta agtgctgttt actatttatt aaagatacat ataatttata gaggaggaat
     5161 aattattatg attattacta ttgttggtgg aggaagcaca tttactccag gtattgttaa
     5221 gtcaattgcg ttaagagaaa aagagttaga gcttgaggaa attcgcttat ttgacattga
     5281 taaagaacgc caagataaag tagctgtagt tgtcaactgg atactaaaag aagatgttaa
     5341 aacagatgtt aaactaactg taactaattg tgaaaaagaa gcgtatacag atgctacatt
     5401 tatatttgca caaatgcgtg ttggtaaata tgaaatgcgt gagcaggatg aaaaaattcc
     5461 attgaaatat ggttgtgtag gtcaagaaac ttgtggttgt ggagggatgg catacggact
     5521 gcgtacaata caaccaatga taaaggttat tgatgatgta gagaaatatg ctaaaaaaga
     5581 ttattggata ttaaattact ctaatcctgc tgcaatcgtt tctgaagctt gtcgtaagtt
     5641 aagaccaaat gcaaggatta taaatatctg tgatatgcca atagctatta tagatatgat
     5701 agcggagtca ttaaatatca atgacgttca taatatacgc tatgattatt ttggtctaaa
     5761 tcattttggc tggtttactt caatagatta taaacataga gacttaatgg aagagattaa
     5821 ggaatacata aaagaaaata aaatattatt accaaaatct tatatagaaa acttaaataa
     5881 aagtcgtagt acaggaaatc gccatgctgt aagtagctgg atacatgttt gggaatctgt
     5941 ttatacaatg ttagaaagct tcccaaaata tctgccaaac acttatttaa catactatct
     6001 tttaagtaaa gaaacagtgg aacattcaaa cataaatcgt actcgtgcaa atgaggttat
     6061 ggaaagccgt gaaaaagact tatttgaagg tgtgagacat tacttagaaa ctggagaaat
     6121 ttctgaaaaa gtattttatg caggttcaca tggagattgg attagtgatt tggcagtttc
     6181 aattaaaaat gatacacgta gtcgtttttt agttataact gaaaatcgtg gagctatacc
     6241 aaacatgcca tatgatgcaa tggtagaaat tccagcatat attggtaaag atggtccaga
     6301 agttattgct cgaggtccaa tacctctatt ccaacaagga ttaatgatgc aacaacttaa
     6361 cagtgaaaaa ttgatagtag aagcttatat agaaaatagt tatgaaaaag ctttacaagc
     6421 attcacatta aataaaacta ttccaagtat gaatgttgct aaagaaattt tggatgaaat
     6481 gatagaagtt aataaggaat actggccaga attaagatag taggataaaa gaaagggttt
     6541 ttatttaata attagatatg ttttcagagt cttttatcaa aatgtattca caaacatatg
     6601 gcaatatatt aaaataaaga gtgatatttt tagaagaatg gggtgatgca atgaacatta
     6661 taaataaaat tttttggcaa gaagatagga ttggcatgat tacaaataat ttagaggcag
     6721 atacacatag ccactgcatg ttacaactgt tcttagggat agaagacagc attgaaataa
     6781 cagtcaatga aaaattagtt aaatgtaatt gtatcatagt agataaaaat atctctcatt
     6841 cattttcagc aagaaaaaaa gtttattact ctgctattat tgaacctaca tctatatatg
     6901 cagaacagct tacctcaaaa atgaatgatt ttggctactg gatatgtgac aatgatggat
     6961 tagagaaatt acgtcaacaa ggaacttttt tgatatataa ctctagtaaa gaacaatatt
     7021 taagatttat ggaaatgctt aataactatt tgaatatacc tataactctc aaacattatg
     7081 atgacagaat tactgaattg ctcaatttat tacgtacttg taattgtgat aatcatacta
     7141 tttcaagttt tgcagataaa gtgtccctat cagcaagtag attatcacat ctgtttaaag
     7201 aacaaatagg gataccttta aaaagctata tcttatttca tcaaatggag tgtgctttta
     7261 gagaattact ttctgggaaa aatgttaccg aagcttctat gactgctgga ttcgatactc
     7321 catcacactt tgcagggaca gttaagcgaa tgatgggaat gcctgtttct ttatctttga
     7381 aagatagcga atttttgaaa gtatattaaa tattctcgtt atataatgaa gttgtactaa
     7441 gaaaggggct ttgttatgaa taaagaatat ttagaggaaa caaaaatgtt aaattataat
     7501 gaaccacaac ttaagctttt ggtatcatca aagaattggt tggaattaga tgattttcat
     7561 aaaataaaat ctatttatga gtttgtacag aatgatattt tatttggata taatgcttct
     7621 gatatgttaa gcgcaacaca ggtattaaat gatggtatgg gacaatgcaa tacaaaagct
     7681 actcttttaa tggcattatt acgtgcagtg aatatccctt gtaggcttca tgcttttgat
     7741 gtaacaaaag attttcaacg aggtgctaca tccaaattga tttcactatt agcaccaaaa
     7801 tatattcttc atacttgggt agaggtattt tatcaagata ggtggattgc tctggagggt
     7861 gtcataactg ataaaaaata tttagaagca attcagaaga agttttttaa tcatggtgga
     7921 acgtttaaaa agtatgcaat agctacaaat gatttgaaaa atacatctat tgattgggat
     7981 ggtaaagata cttttatcca aaaagaagct atcgtgtatg attatggtat ttttccatca
     8041 ccagatgtat ttttttctac tcattcacaa catatgtcaa aattaaaaaa tttcatatat
     8101 gtacatctta tcagaaaaat aatgacaaaa aatgtatgta aggctagaaa caattatatt
     8161 gataaaaacg aatgagtgtt taagaggtaa ctatatagga aatatataga ataattaaaa
     8221 ctgtactggt taaaatgaat aaaaaggagt tatcatagag caaacttaaa ttgttcatag
     8281 gataactcct tttctgtatt aacttctaat aatttgatgt ctttttataa aacatataga
     8341 tacaattaaa ccagtaaatg caagagtagc tgctattgaa aatgcaacat ctattccatg
     8401 aatgttagac agcatgtttg aactcatatg agctttctta gtagcacttg tcattatagt
     8461 aataagtata gcagaaccta tagctccaga tatctgacgg agtgtgttat taattgctgt
     8521 ggcatgagat ataagttctc tatctaatgt tttaattccc catgtagtta atggcattaa
     8581 aaccattgaa ataccaatca tacgaataca atacatcaat gacacatgaa ttaaagatgt
     8641 atctctgcct aaaaaagcga aggataaagt tcctaaaaac aaacatcctg ttcctaatat
     8701 tgatagagca tgaggtccat atttatctaa atgacgtcct gcaattggat tgagtacaac
     8761 catcaatata gcaccaggta acattaatga acctgaagat aatgcagaaa atcctcttac
     8821 agattgaacg tacagaggaa tcattaatgt agcagacatc attgaggcat atacaatcat
     8881 aattaatatt gtagaaataa gaaatggttt atttttaaaa accctcaatt ctaaaaaagg
     8941 ttctttagat tttaattgac gcaatgtaaa taaaattaat gacataattc ctattaatat
     9001 tggtaaatat gtggcaatat tagtccaccc ataattacct tgattagtaa atccaattaa
     9061 taaaccacca aaacctagag atgatagtac tattgaaggt atatctaact ttagtttttg
     9121 agtttcccca acatttctta atagaataaa tgatagtata acatctaata tagcaatagg
     9181 ggaaagaaac agaaataaac tatgccaacc aaatgaatct actaaccatc ctgataaagt
     9241 tggacctatt gctggagcaa atcctactgt aataccaact aaactcatag ctgcaccacg
     9301 tttttcctca gggcataaat agagtataat aacttgtaaa agtggcatta gagaaccaga
     9361 acccattgcc tgcaacacac gagcaatcaa catagttgaa aaattattag aaaaaagtgc
     9421 aataatacat cctatagaaa agatagacat tgatgtaata aataactttc tagtactaaa
     9481 tcgatttatt aaataagctg ttgtgggtac aataatacct gcaataagta gataaattgt
     9541 tgttaaccac tgaccaatat cagcagtaat tttaaaatct gacattatat taggtaatgc
     9601 tgttgacaat agtgtttgac tgagagaact tataaaagca ccagtcatta caattgctat
     9661 tattaaattt ccttttttat tgttactttg ggtttccata atttataacg tctccttttt
     9721 taattgattt ttaatgctgt tcaataaaag tacttttggt cttaaagtgt cttctttttc
     9781 taaatatgca atcattgcat ccataattaa agaatcatat tgttgatttt ttccctcgac
     9841 gtataatttt tttattgctt ctagtaattt aggattttca aggagagaag tatcagtttt
     9901 gattagttct tctatatctt gtatcgtttt aaaaaatagc gatttattat gaagggaaaa
     9961 gtaaacttca tcttcttgtt tatgtaaaaa cattgatttt tcaattaaag gatttagatt
    10021 atccatattt tctacaacta aatctagaat ttgtatagta aatttggcaa taaagtatac
    10081 atttatttct cttttttgta catgaataat gtatataaac tgatgaatta gagcatccaa
    10141 taagaaaact aaatcaaata taattttttc tattttatca ccatagttat caaataagct
    10201 taatttgtat tcattcaata agttagctcg tgattttgtt tgcaatttac ttgtagcctt
    10261 acgttgctct tcagaaaaat tttctcttat ataggtattt atggtagttc tagaaaaaat
    10321 atttttccat acagcaatta tctttctgtt taacttttct gatgatggaa tgtcattatt
    10381 ttcgtttatg gaatgtataa tttctaaaaa cttttcatca taataattga ttatttcgca
    10441 gataaaactt tccttagttt caaaatgctt gtaaaaagta gctttagaca ttttacactc
    10501 ttttacaaca tccgatattg aagtattttg aaaacctttt tggtcaaaaa ttttttgtgc
    10561 aatatttata atgagccttt gtttttctgt cactttatta tctcctttca cgcaaaaacg
    10621 tttagtacta tatagtacct acaagtttta ataaatatat tatacatcat ttggaaaaaa
    10681 aatcaatata tagactgtaa tatgaaattt ttgaaaaatt tgttgttatt tttagtcgta
    10741 taaaaaagaa acttaagagt ttcttaagaa ttgtttaaag aagttgcaat aaattagtgg
    10801 tatgattata gtatgaaaga gaatgtcttg aaatttttct gtatcaaatt ccaattatgg
    10861 aggtgtagta tatgaaaaag aaaatttgtg tagcaataat ttttgctcta tttatagttt
    10921 ttgtagaaat gtttattgat agctctagcg aaggaagtaa aattatgaca gctgaattaa
    10981 gtaaatacag gaaaggcgta aatgtattat caataaacaa tattatagta gcgaataaaa
    11041 aatatagttt gcccaaagat tattctcctc aggaaagcag tgaagcgaga gatgcttttt
    11101 ataaaatgaa taaagatgct caaaagtcag gattaaattt aaaagctttt agtacatata
    11161 gaagttatga atatcaagat agattattta aatcatatgt aaaagaacat ggagaaaaag
    11221 aagcaaatag attttcagct aaacctggag agagtgagca tcaaactgga cttgcttttg
    11281 atattggagg agatgaccag tcttgttggg caaataaaaa gttcaacaat acaaaagaag
    11341 ctaagtggtt gtatgaaaat gcatataaat atggttttat acttagatat ccagaaggta
    11401 aagagaatat aacaggatat atgtatgaat cttggcacta tagatatgtt ggaactgaac
    11461 atagcaaaaa ctttgcaatg aataatttga ctttagaaga atatttacat ataaattaag
    11521 agatggtttg ttaaaattag gttaaaataa aaaataacct agactattag ctgtgtaaag
    11581 tgatataatg taaatacaat ttaagataac tactcgaagg ggagtagctt acacgaaagt
    11641 ggtaattaat cgtcaagaca gtagaaatac tcggttaatt agcaagggat tccttgatag
    11701 caagaccttc aaaataagca ttaggtatgt ttgttttggg gtcttttttt attcaaggaa
    11761 tttttattta aaaaaattta aatttcaagt atacataaga gcccccaaac ataaaataaa
    11821 tttattgaag aggagggcaa caaatgtctt acaaaagtac ttatcaagaa gtaattaaaa
    11881 atttagactc aagtttatct ggtttgagta aagaaagagc agaagagttg ttggaaaaaa
    11941 atggagctaa tgaattaaaa gaagcagata aggttcctac ttataagctt ttcctagaaa
    12001 gttttaaaga cccattagta ataattctat tgatagctgc tttagtacaa atatttttag
    12061 gtgaaacagt ggagtctata ataatttttg cagttattat tataaactct gttctgggtg
    12121 ttgtacaaac aaaaaaagca gaaagttcat tagaaagttt gaaaaattta tctgcaccaa
    12181 acgcaaaggt aataagagac aataaaaaaa tgacaattcc agcaaaaaat ctagtagttg
    12241 gagatattgt attcttagaa gctggcgatt acataccagc agatggaagg cttatagaag
    12301 cacaatctct caaggttgta gaagggatgt taactggaga atcagaacca gttttaaagc
    12361 atacagaaaa aataagtgaa gatgtaagtt taggtgacca gaaaaatatg gtatttagtg
    12421 gtgctgtagt tgtttatggt agaggttctt ttgtagtgac tggaacaggt atggatactg
    12481 agatgggtaa aatagctggg cttttagaaa gtgctgaaaa taaacttaca ccattgcaac
    12541 aaaaaataga ggattttagt aaaaaacttg gtgtaggtat tgtaatactt gctcttttaa
    12601 tatttataat tcaagtagca agaaattact ttatggctgg aaatggagaa atgacacaaa
    12661 ttattttaga ttcattcatg ttctctgtag cagtagcagt agcagctata ccagaagctc
    12721 tatcatcaat agtcactata gtattggctg ttggaacaaa tagtatggca aaaaaacaag
    12781 ccataataag aaaattacca gcagtagaaa ctttaggttc aacaagtgta atatgtactg
    12841 ataaaacagg tactcttaca caaaataaaa tgacagtagt tgattattat atgtatggaa
    12901 ctaataatga tgagttagat aaagaaaaag taaactattc ttatcatgct aaattattga
    12961 ctatggtttc tacattgtgt aatgactctc atataactag tgatggtaaa gaagttggtg
    13021 acccaacaga agtggcttta ataaattatt caagtaaaaa tgatttagat tatgataaat
    13081 taagaagtaa gtatatcaga ataaatgaaa taccatttga ttcagacaga aagcttatgt
    13141 ctactgtaaa tagtatatat ggagattata taatgtttac aaaaggtgct ccagatattg
    13201 tgtttagtag atgtaaatat gctttgaaaa atggttcaaa agtagatata acagatgaga
    13261 taatagaaga atataaacat aaaaatgaag agttttcaaa tagagcactt agagtattag
    13321 cttttgcaat aaaagatgtc ccagaagaag attttatacc ttcaattgat gacgaacatg
    13381 acatgacatt ggttggaata atggcaatga tagacccacc aagagaagaa gtaatggatg
    13441 cagtaaaaga ggcaaagagt gctggtataa aaactgttat gataactggt gaccataaaa
    13501 caacagcagc agcaattgca aaagagattg gtataatgga agaaggagac ttggctctta
    13561 ctggaaaaga gcttgatgct ttgagtgaag aacaactata tgaaaaacta gaaaatataa
    13621 gtgtatatgc cagagtctcg ccagaaaata aaattcgtat agtaaaagca tggcaacata
    13681 agaataatat aacagcaatg actggtgatg gtgttaatga tgcacctgca ttaaaacaag
    13741 cagatatagg aattggtatg ggaagtggta ctgatgtagc aaaagatgct tctgctatgg
    13801 tattggttga tgataacttt gcaagtatag taaatgctgt tgaagttggt agaacagtat
    13861 acagtaatat aataaaatca attacttatc tatttgcagg aaacttagga gctatagtat
    13921 ccatagtatt tgcagtgttt gcaggttggt caaatccatt tacagcttta caacttttgt
    13981 ttataaactt agtaaatgac tctttaccag caattgcatt agggtttgaa aagtcagaaa
    14041 aaaatataat gaataaacct cctagacacc cggatgaaag tatactagca ggaggaacat
    14101 tgaaggcagt tgctataaga ggttgtataa taggtgtagt tacaataatt gcacaatata
    14161 taggattaca agtatctcca gaacttggag tagcaatggc attttcaaca ttgatattag
    14221 ctagaataat gcaaacatta cctgcacgtt ctaataatca aacagtaata aaattaggtt
    14281 ttttcagcaa taaatacgtt ataggagcag ttttagtctg tcttgggctt tactctttaa
    14341 cactaatacc agtgctaaga ggaatattct ctataccaac aacgtttgga ttgtaccagt
    14401 tggggatttg cctagggtta gcattaattt catcaatatt aatggaactt aacaaattaa
    14461 taaatagaag tgaagtaaaa taatcaaaaa attaaagggc atattgattt taacaatatg
    14521 ccttttataa atagtctaat ttattctata ttatcgatta tttggaatgc ttttataact
    14581 tcaggtaatt ctttttcttc aggcattcta aatcttatga aatttttatc aagtccagta
    14641 aaatcacttc cagatataac tcttattttt aatttagcaa actcttgttg taaatctatg
    14701 tccttgtttt tatgttcaac tgtcataatt gatacagtat cactagtttc agctatatta
    14761 aggtttttcc atggaattag taattgattt tttatatctt ttgttttttc ttttaattct
    14821 tctataaatt gaacatcatc taataaattt gctgcaatac tacgagatat ttcagacact
    14881 tcgtaaggag tagaaatctt tttaataggg ctaacaagtt gttctggaag aactgcatat
    14941 cctgctctta gtcctgctaa accaaatcct tttgaaaatg tttttaaagc aattacatta
    15001 tcataattat ttagaagctt gacagctgaa ttttcttttg gcatgtattc accataagct
    15061 tcatctacca taactgctat atcatattta gctgcttcct ttactatatt ttcaattgat
    15121 gataaaggta taatttgtcc tgtaggattg tttggattgt ctatgtaaat tactttatat
    15181 tctggattta ttttttcaat aaattctttt tcattaaact tgaaattatc ctctttcttt
    15241 aaaagaacat aatcataagt tgcaccatgc atttttatat ctgtctcata ttctgagaac
    15301 tgaggtgaat atcctaaaac tttatctccc ttttcaataa atagtctatt taaaaggtaa
    15361 ataacatgta tagaaccatc tgccaaacaa attctgtcag tatctaatgc tataaaatct
    15421 ttccaataat cgactatatt atcttttaaa gcaattgaat gaggatatcc tcttatcata
    15481 tcaaatttta aagcattaaa agcttctaca gctttgcttg agtaagacac ggtatttata
    15541 ccttcaccac agtctatctc gaatacatct tctgaatgtc cagattctat tgcataactt
    15601 tttttcaact gcatttcaat agctttgtta atttttagtt tcatacttta tcactcctta
    15661 tttagtcaat aataaatgtt ataaaagttg ctagatttaa aatattatga aaatatataa
    15721 tagtaaaaaa tgaatataaa tattataata gctcacttta tttaatatga ttatatatca
    15781 tatattgtaa aatttctagc attataagac tataaattga aaatattcag aaaaatacta
    15841 caagaaacga tttattttgc tcttaaaata agttaaatga atgttattaa ttttgtaaat
    15901 tgaaatagtt taagtataaa gttatgatat aatctaataa gggtaattct ttagattata
    15961 ttaaggaaat ataggtatac aataaaaagt tatggagttg gattatggga tataaattaa
    16021 ttgtaacaga tatggatgga actttattag gcaataatca taaagttaca gatgaaaata
    16081 aaactgcact acaaaaagtt ataaaaagcg gtattaatgt aactttagca acaggaagag
    16141 ccttcgattc agctaaatgc aatgtagatt ttcttaaaga ggacatgcca ataatagctt
    16201 gtaatggttc attaataaga gagcaaaatg gaaatataat atattcaaat aagattgata
    16261 caagaacatg tttaaacatt ttagatgtat tagataaata cgatatttac taccaatgta
    16321 atagtataga ttctatgctt tcgaaaaaaa tagaaggacg tgaagataga ttaagtgtat
    16381 ttttaggctc tgaaacagaa gtaattgtaa aagatgattt gagagaagaa atctttaaaa
    16441 aagatatact aaaatttgta atcatagaag aaaaaaatcc atctattttg gatgaaataa
    16501 gaaaagaatt gagaaaagtt caaggaataa agataacttc ttcatggcca aacaatattg
    16561 aagtcatgaa tgaaggtgtt gataaaggta atgcagttaa aattttagct gaaaaaatga
    16621 atatagacag agaagatata attgcatttg gagacaatta caatgacata gaaatgataa
    16681 agtttgctgg tcttggggta gctatgggaa atgcagaaga attgattaaa caagaagctg
    16741 attatgttac agatacaaat caagacagtg gagttgcaaa ggcaatttat aaattcctag
    16801 agttaaaaga atcacttagc tcttaaaaca gtatataata ggaaaaaata ggttgactat
    16861 tttgtagata gtaattataa tacaaataga taaaacataa aaatttagat tcagtgaaaa
    16921 agagagtaga tatagagaaa atctcagcga attgggggat ggtgtgagcc taatgataac
    16981 actttatatt gaaaagaact ttggagaata ctacttgaaa ttcagtatgg tagtaggtaa
    17041 tctcgcctta agagaataga gtgggtaaac attgtttacc aactagagtg gtaacacgga
    17101 atatttcgtc tcttggtctt tttgaccaag agatttttta gtttcattga gttacaagac
    17161 ttataattaa aatacaaaaa attaaaatat gaggtgaaag ttatgaaatt atggggcgga
    17221 cgctttagaa aagcggaaaa tcaacttatg gaagaattta ataaatcttt tggctatgat
    17281 tgtgtactgt acaaaaaaga tatcgaagga agtgtagcac atgttcatat gcaagtaaaa
    17341 tgtggacttt taacagaaga agaaggaaag tctataacag aaggtttaaa agggatactt
    17401 gaagatgtag aaaatggaaa gttagtattg gatggagagt atgaagatat acatagtttt
    17461 actgagataa atttaattca aagaattgga gatgttggca agaagcttca tacagcacgt
    17521 agtagaaatg accaagttgc agttgatatg agattgtatg ctaaagaaaa agcaaatgat
    17581 ttggtgggtt taatatctga atttaaagct actataaaag atgttgctga caaaaaccca
    17641 gtgatgatgc caggctatac tcatcttcaa agagctcaag tagtgacatt taagcatcac
    17701 ttaatggcat attatagtat gtttgataga gatgagaaaa gaataaaaaa tgctatagaa
    17761 atattagatg aatcaccttt aggctgtgga gctttagcag gaactacgca tgatatagat
    17821 agaagtataa cttgtgaaca attaggattt aaaaaagtag tagataattt tatggatgga
    17881 gtaagtgata gagattattt actagagcta atgagtgatt tttcaattat aatgatgcac
    17941 ttaagtagat taagtgaaga gttgatactt tggagttctc aagaatttgg atttgttgag
    18001 atagatgatt tatatacaac tggaagtagt ataatgcctc agaagaaaaa tccagatggt
    18061 gcagagctta taagaggtaa gactggcaga gtatatggaa acttatttgg tctttttaca
    18121 gtaatgaaag gaataccatt agcatataat aaagacatgc aagaagataa agaaggtttt
    18181 ttcgatagtg tacatactct tgaaatgtgt atacaaataa tggatagaat gatagccaca
    18241 ctaaaggtaa atgaggataa aatgaaacaa gcagtaaaaa atggtttttt aaatgcaaca
    18301 gaagttgcgg attatttggt taaaaataat gttgctttta gagatgcaca tggtatagta
    18361 ggaagtatcg ttatatattg tgaagataat aaaaaagcaa tagaagattt aacattagaa
    18421 gaattacata agttttcaga tgcttttaag gaagatattt atgattttat cgactatgag
    18481 agtatattaa ataagggaat aaaaaagaat ttaaaataat gtatttattt ttaaaagctg
    18541 taaatttgaa tgttatcatt taaatttaca gctttttatt gtgtaagatt ttattatata
    18601 aaagtaagaa acttaaaagg agtagtattt aaaataaata caaataaaaa attattatta
    18661 aaatgataat taatatcaat aagtattgac aaaattattt ctaaatacta ttatatataa
    18721 tataaataat aatgaaaatc aatatcaaat aaatgggagg aaatctaatg aacttattag
    18781 tgattggtgg tcatgaaaga atggaaaaag attatatggt tatggccaag aaaaaaggtt
    18841 ataagacaaa ggtatatact accatgtcat ctaaattaaa taattctata ggaagaccag
    18901 atgcagttgt catacttaca tcaactgttt cacataagat gtctagaacg gttgagtctc
    18961 aagctaaaaa acaagatata ttaatcgtaa ggcataaaaa tagtagtaag gtagcattta
    19021 atgaatgtct agatatggtt gatgaatgtc tgggaaattg ttcaaaatgt aagcttaaag
    19081 acaaacaaaa acaaaaaaat atataggtat aaaaaataca taaacataaa attaaaagta
    19141 ctgagataat agttataatt attatctcag tacttttaga tttataagga taagtatatt
    19201 ataaattttt tattataatt tccaactact tgttactttt tcatattttt tttacttcta
    19261 gctttttttc tttttttctt ctttatattt gaagctatta taaataaaac taaaagcact
    19321 agaatacctg caataatctt aacaaaagtc attaaaatat tatttccagt aaaaaatgga
    19381 agactgctat caagattatt ttttgcaatt aagtctatac tgttttctag tttaccatta
    19441 ttatatacat ttaaagtacc aaccttagct ccttttttta taggcaaatc aattttattt
    19501 aactctgttt tggtatcata attgccttga gattcatctt tttctaagaa tatcctataa
    19561 ctattttttg gttcataaat caattcatgt tgttttgtaa acaatacttt tttagtatct
    19621 gtgtattttt ctttatcaac aatagtagca cacttaaagt tatcaaatcc ataatcaatc
    19681 agaatacgtg agtctaaata taaatcagca ttagttgaat taaatacagc aacgataact
    19741 cttctgccat cttttacagc actcgataaa agacatttac cagcagcatc agtatatcca
    19801 gtttttatac catcaactat atcatatttt atatttatag gttgaccttt gtatgtaatc
    19861 ttatcatgtg atgtcaaaaa cttattagtg tttacaaagt atctctcata aggatatgct
    19921 tttgtagctt caaatttaag tgacttagtt tttacaattt gtctaaaagt atcattattc
    19981 atagcttctc tagctatgag agccatatca tgagctgttg tgtagtgatt tgggtctggt
    20041 aatccatgag gattattaaa atgagtatta gtagctccaa ttttttttgc ctcagagttc
    20101 atgaggtcaa taaatttttg tacattgcca ccaccaacat atctagccaa tacaaaagca
    20161 gcatcattag cagaatgaac caaaagagca tctagtaact gctttacagt aaaagattcc
    20221 ccatttttta gatacatact agagccatct atttgaggta aatctttaat ttctattact
    20281 tgatttaaat ctttaacctc ctttagaact aaacaagctg tccatacttt agttgttgaa
    20341 gctggataaa gcttagaatt tccatcctta ttatatagta ttttgccaga ctcatagtcc
    20401 attaaaactg catgtttagc aactatagct ggttcattat ctgcaaaaga gatattagag
    20461 cttcctaaaa atgaagtaaa tatcaataaa cagattaata aaagtgataa atttcttttc
    20521 attgtgagcc tccaaatttt aaattcatct tttagtatag catatatttg tcaataatta
    20581 aaagaaagga gtgattaata tatgaaaaaa aaggtaaaat ttatttcgtt ttttgtttta
    20641 tttattttaa taatatcatc aataatcgtt tataaatcgc ttattttcaa agatgatatt
    20701 tatgttgtaa gtaaaaaaaa tttacctaat gaaaaaacac atgttgtaga gaatgaaatt
    20761 aaaggtgaag ataataatca caataaagta actaaagaca tagataaaaa ttttcaaaat
    20821 aataaaataa ctatatacat aagtggagca gtaaatagac ctggaattgt aactatagag
    20881 tcagacaaga gactttatga tgctgtagaa ttactaggag gaacgacaaa agaagcagat
    20941 ttaaatggag tcaatctatc agtaagatta gaagatgaac aacattacat aatacctaaa
    21001 ataggtgaag caacatctgt tacaagtaat gatgattcca ataaaccaaa tcagaagaat
    21061 gagtcaagag gtgagcctaa aaataaatcc aataaaaata tatctaacga atcaaaagta
    21121 aatataaatg tagcgactat agaagagtta gattctctac ctggggttgg agaagctaca
    21181 gccaataaaa ttttacaaca cagagaagaa aatggacagt ttagttcaat tgaggaaata
    21241 aaaaatgtaa atggtattgg agataaaaag tatgaaaata taaaagattt aatttgtgta
    21301 gactaaaata taatacaaaa cttacatata gatatgttat tataaatatg tatgtatatg
    21361 tgtgtacatg tggaaattta gtaaaggagg cacttttatg aaaaaagaac aaaaaaaact
    21421 tacagagatg acaacagcag gtggttgagc cgctaaaata gggccagagg ttctggcatc
    21481 agttttatct caattgccta aaaatgataa tatagaaaat ttattagtag gactagatac
    21541 agcagatgat gctgcagttt ataaattaaa tgatgatatg gcacttatcc aaactttaga
    21601 cttctttaca ccaatggtag ataacccata cgtatttggt caaattgctg catcaaattc
    21661 actatcagat gtatatgcta tgggaggcaa acctttggtt gctatgaata ttgtttgttt
    21721 tccatcatgc catgatatgg atgtacttgc ggaaatatta aaaggtgggt ttgataaagt
    21781 gaaagaaagt ggagcacttt tggttggagg gcatactgta gatgacaaag agcctaaata
    21841 tggtctttca gtttctggga ttgtaagtcc aaataaagtt ttatcaaatg ctactgcaaa
    21901 acctggtgat aagttaatta taacaaaacc aattggagtg ggtgtattaa atacagccat
    21961 gaaagaaggt atggtagaac aacatatagc agataaagta atagaaataa tgatacattt
    22021 aaataaatat gcagcaatga gttttgagaa atttgatgta aattcggtta cagatataac
    22081 tggttttggt ctacttggtc atactttaga aatggccaaa gcttctgaag taagtattga
    22141 aattgaatca aaacatgtac caattattga aggtgcaata gaaatggcac aaatgggaat
    22201 tatacctgct ggtatgtata aaaatatgca ttatgtatct aaagatgtag aagttgtagg
    22261 aaatatagaa gttgcagttc aagatatttt atacgaccca caaacatcag gagggttgtt
    22321 aatttcagta aaagaagaac ttgcagagga acttgtaaaa gatatgaaat taaatggagc
    22381 aatagaagct aaaattatag gttctgtagt tccaaaaggg gaaaaatata ttacagtttt
    22441 ataaaatatt atagagaaag agtgattgtt ttgagcaaaa gagaattgtt tgcaatgttg
    22501 ccttcagttg atgaagtatt aagtgataat agaattgtag aaataataaa tgaatatcct
    22561 agaagtttag ttttagaatc tgtgagagaa gtaatagatt taaaaagaca atttatatta
    22621 agattaaaag aagatgcatc aaactctgta acaatagagt ttggtgaaat aatagaatct
    22681 gcaatagaga gagttaagtt aaattactca ttatctttaa aaaaagttat aaatgcaact
    22741 ggtacagtta ttcatacaaa tttagggagg tctttactta gtgaagatat aaaagatgaa
    22801 ttatggtgtg ctgcttcaag gtattcaaac ttagaatacg atttggataa tggagaaaga
    22861 ggttcaagat actctcattt aactagtaca ataaaaagac ttactggagc agaagatgta
    22921 ctagttgtaa ataataatgc agcggcagta cttcttgtac ttagcacaat ggctaaagga
    22981 ggagaagcaa ttgtatctcg tggagaatta gttgaagtag gtggttcatt taggattcca
    23041 agtataatgg ctcttagtgg tgcagaatta gttgaagttg gttcaactaa taaaactcat
    23101 ttgaaagatt acaaggaggc cattacagaa gatacaaatg tattgatgaa ggttcataca
    23161 agtaattata gaataatggg attcacagag agtgtttcta tagaagaatt ggttaattta
    23221 ggtaaaaaat ataaactacc tgtaatagaa gacttaggaa gtggagtttt tatagattta
    23281 tcaaaatacg gtttatctta tgaaccaact gtattggatt ctattcgaca aggtgcagat
    23341 gtagtaactt ttagtggaga taagatgctt ggaggtccac aagcaggtat tatagttggg
    23401 aaaaaagagt atatagaaaa aatgaagaaa aatcaactta ctagagcact tagagttgat
    23461 aagcttacaa tatgtgctct tgaagcaaca ttgagaatgt atttagatga gacaaaagct
    23521 atagaaaata tacctacttt aaaaatgttg acatataaaa ttgaagaatt agaagtaaaa
    23581 gcaaataaac tatttgaaaa aataactgct ttaaacttaa atgcaaacat aaatatagaa
    23641 gatggatttt cacaagtggg aggagggtct atgcctcttg aaactataag tacaaaggta
    23701 atatcaataa ctcctgaaca tatgaatgtt tcatctttag aaaaaaaact aagacttagt
    23761 gaagcacata taatagccag agtgtatgat aataaatatg tactagatgt aagaactata
    23821 tttgatgatg aatttgatgt aatagttgcg gaattgagaa aagcatttaa ttaaataaat
    23881 ttgagctaat aaatattaaa tttagaagta taagtatgaa gttttgatta aaaaatagct
    23941 acacacactt gtgactttag tcatagcagg tttagagggg gcaaaaaatg aaaaatgtag
    24001 taattggaac agcaggtcat attgaccacg gaaaaactac tcttataaaa gcattgactg
    24061 gaagagagac tgatacatta gatgaagaaa aaaagagagg tatttcaata aacttaggat
    24121 ttacttattt tgatttacca aacaatagaa gagctggaat tgttgatgtc ccaggacacg
    24181 aaaaattcat aaaaaacatg ttagctggag catcaggaat tgatatagtt ttacttgttg
    24241 tagcagcaga tgaaggggtt atgccacaga cagttgagca tttagatatt ctaagctttt
    24301 taaatatcaa aaatggaata gtggttctaa ctaagtctga tactgtagat gatgaattta
    24361 gagagctagt aaaagaagat atttgtgaaa aaataaaagg aacttttctt gaagatgctg
    24421 aaataattga agttgattct atatctaaaa gaggtataga cactcttatt gaaaaaatag
    24481 ataaaatgag tgatgatata gaagataaaa atgaaaactc tccagcaaga cttaatatag
    24541 acagagtatt ttcaataaaa gggtttggta ctgtaataac aggcacttta atagaaggaa
    24601 agataagtat agaagatgat ttagttatat atccacaggt attaaaaaca aaaataagaa
    24661 gtatacaagt tcatggagaa aataaagata cagcttatgc aggtcaaaga acagcaatca
    24721 atatatcaaa tgtaaaagtg gatgatataa aaagaggaga tgttttagct gcaccaaact
    24781 cattagagga atcaatgatg ttagatgtaa aattatcatt ggtaaatcat ttagataaag
    24841 gattaaagca ctgggataga ttaagattgt atcatggaac tagagagata ctttgtagag
    24901 cagttccact agataaagaa ttaattgaaa gtggagaaag tggctatgtt cagttgagac
    24961 ttgaagaaag catagtatct aagaaaggtg atacgtttgt agtgagacgt tattctccaa
    25021 tggaaactat aggtggaggt gtaataattg acccatctcc taaaaaacat aagaaatttg
    25081 atgaaaaagt tatagaagca ttaaaaataa aagaaaaagg cgaactaaaa gatataatag
    25141 aagagtactt aaaaaggaat ttaaaaaatt atccaaatat aaaagaaata atgagctata
    25201 gtggtgctca tgaagaagat gtaaaaagag cgcttgaagc acttatatca gaagataaag
    25261 tatttattat aggaaatatg tatatgcata taaatcaata caataaatta aaagaaaata
    25321 caataaagct tttaagtgag taccataaga agtataggct tagaaaaggg atactaaaag
    25381 aagaagtaag gtctaagata gagagtaatt ttaagactag ggagatggat attttattag
    25441 aaaaattgtc tacagaaaat gctataaaaa tagaaaataa tatagtatct ctattagatt
    25501 ttgaggttat tttaaatgat aagcaaaaag aaatagcaaa aaaaatagaa aaaagattaa
    25561 aatcatgtgg agtttcttct atactgacaa tagatgaagt ttctgaagga aatcataact
    25621 atgctgaagt attggagtca atgataggta ataaagtaga gaaattagat gatttgtata
    25681 taatggacaa agatatttat gaaaatgcaa agaacatttt gataaattat ataaaagaaa
    25741 ataaagaaat aacattagga gaatacagag atttgattga ttctagcaga aagaattgca
    25801 tgattatatt ggaaaacttc gatagaaaca aaataactaa aagagtagaa aacaaaagaa
    25861 ttttgtttta gcagaaaatg tctgtttttg ctttaaggta taaagaagga attataatgt
    25921 cgagtatcgt ataattaatt agtataaaat
//
LOCUS       DAGQWR010000052        25653 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.052, whole genome shotgun sequence.
ACCESSION   DAGQWR010000052 DAGQWR010000000
VERSION     DAGQWR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.052"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            260..1126
                     /locus_tag="MIW95_001818"
     CDS             260..1126
                     /locus_tag="MIW95_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY3151146.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            1368..2177
                     /locus_tag="MIW95_001819"
     CDS             1368..2177
                     /locus_tag="MIW95_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151147.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKKGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGQGSYTFFVDEGT
                     AMEYIGEFKEDKKNGKGNFVFSNGNEYIGHFEEDMMNGIGNMLYNSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            2320..3384
                     /locus_tag="MIW95_001820"
     CDS             2320..3384
                     /locus_tag="MIW95_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBY3151148.1"
                     /translation="MKRLIALVLVILIVLFYSPELIEEGYDLKEKFFQSEIWNEFLDK
                     ININNNSKKDKKEQNQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     KLYPDDREELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HINKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            3412..4131
                     /locus_tag="MIW95_001821"
     CDS             3412..4131
                     /locus_tag="MIW95_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY3151149.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVKNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            4155..4739
                     /locus_tag="MIW95_001822"
     CDS             4155..4739
                     /locus_tag="MIW95_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBY3151150.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGYVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIIDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLENFITEARERVHM"
     gene            4741..6090
                     /gene="pabB"
                     /locus_tag="MIW95_001823"
     CDS             4741..6090
                     /gene="pabB"
                     /locus_tag="MIW95_001823"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBY3151151.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            6080..6823
                     /locus_tag="MIW95_001824"
     CDS             6080..6823
                     /locus_tag="MIW95_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBY3151152.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            6857..7558
                     /locus_tag="MIW95_001825"
     CDS             6857..7558
                     /locus_tag="MIW95_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151153.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISNIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELASKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            7584..8156
                     /gene="folE"
                     /locus_tag="MIW95_001826"
     CDS             7584..8156
                     /gene="folE"
                     /locus_tag="MIW95_001826"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBY3151154.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            8222..9013
                     /gene="folP"
                     /locus_tag="MIW95_001827"
     CDS             8222..9013
                     /gene="folP"
                     /locus_tag="MIW95_001827"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBY3151155.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELEIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            9024..9389
                     /gene="folB"
                     /locus_tag="MIW95_001828"
     CDS             9024..9389
                     /gene="folB"
                     /locus_tag="MIW95_001828"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBY3151156.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            9382..9888
                     /gene="folK"
                     /locus_tag="MIW95_001829"
     CDS             9382..9888
                     /gene="folK"
                     /locus_tag="MIW95_001829"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBY3151157.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVASTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTVKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            9869..10654
                     /gene="aroF"
                     /locus_tag="MIW95_001830"
     CDS             9869..10654
                     /gene="aroF"
                     /locus_tag="MIW95_001830"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBY3151158.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLKILKAVKDEVGMAVITELMDVRD
                     IDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            10909..12699
                     /locus_tag="MIW95_001831"
     CDS             10909..12699
                     /locus_tag="MIW95_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBY3151159.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLDCGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            12717..13883
                     /gene="rpoD"
                     /locus_tag="MIW95_001832"
     CDS             12717..13883
                     /gene="rpoD"
                     /locus_tag="MIW95_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBY3151160.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            14048..14737
                     /locus_tag="MIW95_001833"
     CDS             14048..14737
                     /locus_tag="MIW95_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBY3151161.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            14751..15848
                     /locus_tag="MIW95_001834"
     CDS             14751..15848
                     /locus_tag="MIW95_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBY3151162.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            15910..16482
                     /locus_tag="MIW95_001835"
     CDS             15910..16482
                     /locus_tag="MIW95_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBY3151163.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEVLEEMNIEVDIFRVSDEVAKT
                     LPQYYLINYKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(16542..17009)
                     /locus_tag="MIW95_001836"
     CDS             complement(16542..17009)
                     /locus_tag="MIW95_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBY3151164.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(17395..17871)
                     /locus_tag="MIW95_001837"
     CDS             complement(17395..17871)
                     /locus_tag="MIW95_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBY3151165.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLRVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(17991..19082)
                     /locus_tag="MIW95_001838"
     CDS             complement(17991..19082)
                     /locus_tag="MIW95_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBY3151166.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDVDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNKLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(19075..19464)
                     /locus_tag="MIW95_001839"
     CDS             complement(19075..19464)
                     /locus_tag="MIW95_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY3151167.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            19656..20102
                     /locus_tag="MIW95_001840"
     CDS             19656..20102
                     /locus_tag="MIW95_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151168.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEDIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            20366..21091
                     /locus_tag="MIW95_001841"
     CDS             20366..21091
                     /locus_tag="MIW95_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3151169.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCKYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            21078..22322
                     /locus_tag="MIW95_001842"
     CDS             21078..22322
                     /locus_tag="MIW95_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3151170.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMKNIDYGQKILSKYNYDKSIKINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            22402..23079
                     /locus_tag="MIW95_001843"
     CDS             22402..23079
                     /locus_tag="MIW95_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151171.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            23080..25641
                     /locus_tag="MIW95_001844"
     CDS             23080..25641
                     /locus_tag="MIW95_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151172.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDQNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIKKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGESYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGFGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNDFNFYDEISSSESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
BASE COUNT        10500 a         2559 c         4543 g         8051 t
ORIGIN      
        1 taagcagtat catattatgc tagaaaaagt ttcatttaag gcataaatga gatgatttga
       61 acgtttttaa agattagaat ataaaagatt gcactagaca tatatgaaaa agataatata
      121 atagatgatg taatagatga cagttcaaga aattataaaa aaaatttatt tgaaatatta
      181 tctattacat caggaataat atatgtaaga gtaagctttt aaaattctaa agatttagaa
      241 agattgaagg gagaaagaaa tgagtgaagt taagaatacc ctacaaaaac atccagtttt
      301 aagatattca acaatgctat ttgggatgtt gatgacatct ataggaataa atgggttctt
      361 aagaccagca catctgctta gtggaggtgc tacaggtata gcaacatcta taaactattt
      421 gacaaatata aatgtaggtt tattaacatt tttaatcaat attccaatat ttatcttagg
      481 gttcatatac cttgaaaaag agttttgtat ttcaagtttg gtaaatatga ttgtattttc
      541 attattatta ggagcaactc aggaaataag taatattata cctatacatg atatattgct
      601 tcaaagtgta tatggtggta tattatctgg tttaggagtc ggagttgtat ttagaactag
      661 gtcttctcaa ggtggtacag atataatagc tgcaatacta aaaattaaga agaatataga
      721 aatgaaagac actgctttag caataaatgg cttgatagta cttacaggaa gttttttatt
      781 tgggttggat ttagcattat acactcttat aggtctattt ctaaatgcgt attcaatgag
      841 ttttattaaa gatgcaatga attaccaaaa atctgttatg gtaatgtcaa atgaagtcga
      901 tttaatagca gaagatataa tgaagagttt agttagaggt gtaacttttt tagatgcaga
      961 aggtgcatac actcatcaaa aaaagaagat tatatataca atagtttcat caaatgagat
     1021 accaaagata aaagatatag ctttgaagta tgataaaaag gcctttatat ctgtaaatga
     1081 tgttacagaa gtaaaaggaa gagggtttaa agcaaaagat ttatagaaaa ttttacaagc
     1141 tgggagttta taatgtaaat ttgtatatta tatctctcag cttatttaaa tttagtatat
     1201 gaaaataaag gttttatcaa tgatttgtgt atcactttaa aagatatcat tttaaaatta
     1261 aaattgaaaa aattactcaa tatgtagaaa acataaccac tattaggata taataaattt
     1321 aatagattta gtaaaatatc taggaataac gttggaggaa tagcattatg aatttatcta
     1381 aagaatatgt taatttgtat gataattatt tgaaatatat aaatgaagta aacaaagaga
     1441 ttagaagtat aaagaacatg aagaaacgtg ttttaaaaaa gggcgatttt gctccagaaa
     1501 atgaagtatt agaattggaa ggaaaaacaa tacgcgatat agatgatgtc gatacaaaaa
     1561 aaccaaaaaa taaaatatat aaattttcaa atggggatgt gtatgtagga aaatttttag
     1621 aaggaaagat ggaagggcaa ggatcatata ctttttttgt tgatgaagga acggctatgg
     1681 agtacatagg agagtttaaa gaagacaaaa aaaacggaaa aggaaacttt gtattttcaa
     1741 atggaaatga atatataggc cattttgagg aagatatgat gaacggtatt ggaaatatgt
     1801 tatataatag caaagatgaa tatattggaa cttggaaaaa tggaaaaaaa gatggaaaag
     1861 gtatttataa gtggagtgat ggatgtatat atgcaggtga atttaaaggt ggaaagatgg
     1921 aagggtatgg tatttgctat aatagtaaag gggaagtact ttatgaagga gaatggaaga
     1981 ataatttgat tcatggtaaa ggaacttata tatgggaaaa aggtaaaaag tatataggtg
     2041 aatttatgca tggtaaaaaa catggacaag gaacttttta tctaaataat gaattggttt
     2101 atgaagggac ttggaaattt gataagccat ccatcttcga cagaacatta gatgaaatat
     2161 tttcattaag actatagctt tatctattaa tagataaagc ttttttctaa tacttactgt
     2221 aaataagtat gaatgatatt gattagtata cactatcaat attaactcaa taaatgtgat
     2281 attaaaaatt tttgattgat tttaagaagg agggatatat tgaagagatt aattgcttta
     2341 gtgttagtaa tacttattgt tttattttat agccctgaat tgatagaaga aggatatgat
     2401 ttaaaagaaa aattttttca aagtgaaata tggaatgaat ttttagacaa aataaatata
     2461 aataataatt ctaagaaaga taaaaaagaa caaaatcaaa atttacaatc aaataatact
     2521 gaaggggaga acttaggaga gtcagatata aagaattttg aaaaaataag cttaggagat
     2581 aatttaagct atgttttatc aagtattggt aaacctggta gaattgatat tagtgaatat
     2641 ggttttgatt ggtatgtata taatcaatat ggaaaagaat ttgcaatggt cggacttgaa
     2701 aatgatgaag tagtagcact ttatagtaat tctattaatt catgtgaaaa tcaagatata
     2761 aaactaaatc aagatagaca aacagtgaga actaagatta caccattaaa atataagaga
     2821 aaaggaaata ccagatatat aataaactca gaaaatcaat atgatataat aagtaaagaa
     2881 ggtaagtata ttacaatttt ctacgatatt catgaagaaa atagagtttg ctcatattta
     2941 ataattgata aaagtactga agatgagttt aaaaaattat atccagatga tagagaagaa
     3001 cttaaaaaat gttttgaact tgaagttata gaccttgtaa atagtgttag aaatcaaaga
     3061 ggattaaact cattgagata cagtgaacag gcaactttga gttcaagaaa acatagtgaa
     3121 gatatgagag ataataattt ttttgaccat ataaataaga agaatgaaac tccatttgac
     3181 agaatgaaaa gagaaggtat agtatatact tcagctggag aaaacatagc agcaggacag
     3241 attaatgcaa tatatgcgca tgaagcttgg atgaactctg aaggacatag aaaaaatatt
     3301 ttggggaact ataataatat tggagttgga gtaatatttg gaggaagcta taaaacatat
     3361 tacactcaaa atttttataa ataactaaaa tatttgaaag gaagttaaaa aatgacatca
     3421 tggaaaaaaa agacagtata taagtgctta atagcagtag tcttgttttg tggaatagtg
     3481 ttaatatcta attttagtaa agtttctgct ttgatgatgg atactaatgg aaatgttttg
     3541 attaaacatg gttcaaggga aaaaaagcta atagcaatta cctttgatga tggtcctcat
     3601 cctaaagaaa ctagtcaagt tttagatgta ttaaagaaat acaatgtaaa agctactttt
     3661 tttatagctg gaaaacatgc aaaatggtat aaagaaccat tagtaagagc aagtaaagaa
     3721 ggtcacgaga taggaaatca tactttcaat catcctgata taagtaattt aagttctagt
     3781 cagatagaag aggagattgt taagtgtgaa gacattttga aagaagtaac tggaaaaaaa
     3841 cctacattat ttagaccacc atttggaagt tatagagaaa aagacttaat agaaattgca
     3901 aaaaagcatg actataaagt tgttttatgg acaggtgtag atgtgaaaga ttggaaaaat
     3961 ccaggagcta atagcattgc agataaaata attaataaag taaaaaatgg agatataata
     4021 cttttgcatg attatgcaac taatgataca gttgaagctt tagatatgtt tattccaaaa
     4081 atgattgaaa aaggttttaa atttgtaaca gtttctgagt tgataaaata atttttaggg
     4141 gggagattta cagtatgatt cttatgatag acaattatga ttcttttgta tacaacctag
     4201 tccaatatat agaagaatta ggggaagcag ttgtagtaaa aagaaataat gaaatcaaaa
     4261 taagtgacat tgaagaacta aatccagagg taattgtgtt atctccagga ccgtgttctc
     4321 caaaagaagc tggtatttgc atagatattg ttgagcattt taaggggaaa aaacctatac
     4381 taggaatttg tttaggtcac caaactattg gatatgtttt tggaggagat attataaaag
     4441 cacaacagcc tgtgcatgga aaagtatata gtatcaacca tactaataaa ggagttttta
     4501 ggggacttaa aaatccttta aatgtcacta ggtatcattc actaataatt gattccaata
     4561 cagttccaaa agaattagaa attacagcaa ttactgataa aggggaaata atggggatta
     4621 ggcataagaa atatttaata gaaggagtac agtttcatcc agaagctatt ttgtcagagt
     4681 atggtcatga aatgcttgaa aattttataa cagaagctag agaaagggta catatgtaat
     4741 atgataagag agataaatac taaattaaat tcttttgaga tatttactat ttttagaaat
     4801 gaacatgaca gttttatatt agacagtgct atggataaag aaaaattggg aagatattct
     4861 tttataagta gccaaccatt taaggtatta aagtataagg atactgatga aaatccttta
     4921 gaagttttaa aagaagaact acataagtat agggttgtaa atgatactaa tcttccattt
     4981 gtaggaggtg ctgtgggata tttatcttat gatttaggta attacataga aaatttacct
     5041 agaactgctg tagatgatat tgagatgcct gatatgtatt ttggatttta taatcatgta
     5101 atagttatag accatcttgt gcaaaaaact tatatagcta ctccaaacat agatatcgaa
     5161 ttagaagaaa agataataga tgatatagaa caaagaatat taaaagaaga gaaaaaaggt
     5221 atagatagca tatgttatga agaaaaagaa gtaacaccta taagacttaa atctaatttt
     5281 acaaaggaag aatttaaaaa tgcagttcaa agtgttagag agtacataag acaaggagat
     5341 atatatcaag ctaatttaac acaaaggttc agtggagaaa cagaacttac aagctttgaa
     5401 ttgtataggg acttaagaag atttagccca gcaccatttg gggcattctt aaactttgaa
     5461 gatacacata tattatcaaa ttctccagaa agatttataa gatgtgttaa taagagaata
     5521 gaaactagac caataaaagg tactcgtcca agagggaaag ataaagaaga agatttaaga
     5581 cttcaacaag aacttagaaa tagtgaaaaa gatagagctg aactacttat gatagtagat
     5641 ttagaaagaa atgatattgg cagaatatct aaaactggaa gtgttaaagt tccagagcta
     5701 tttgtaattg aaccatatgc aaatgtaaat cacttagttt ccacagttgt aggtgagtta
     5761 aaagatgata aagatgctac tgatgtaata aaagcaactt ttccaggggg gtctataaca
     5821 ggagcaccta agattagagc aatggaaatt atagacgaat tagagccaac gcaaagaaat
     5881 gtatatactg gttcaatagg ttatataggt tttaatggtg atatggattt taatatagca
     5941 attagaacaa taatcaaaaa tgacaaaaaa gtctattttc aagttggagg aggaatgact
     6001 tgggattctg acccagatga agaatatcaa gaaacacttg ataaagctaa atccataatg
     6061 aaggctttga ggggatatta tgaagaataa tgtaggtttt gatagtagtt taagtaaatt
     6121 tggaattggt ttgtttgaaa ctattagagt taaaaatgga atagcagtag atttagatat
     6181 ccatatagaa agaatgctaa gttcaataaa ttgtttggat ttagatataa actataaaaa
     6241 agatttcttg ataaatgaaa ttgttacata tataaaaaaa gaaaatgtca ttaataaagc
     6301 acttagaatt actgtttttg atgaaggtta taatatatct attagggata ttccatataa
     6361 tcaagagacg tatgagaagg ggtttaaatt agctatatcg ccaatagtta gggggaatag
     6421 tttaattcat agacataaga ctactaatta ttttgaaaac atatatacca aaaatatcgc
     6481 aaataagact ggttataatg atggtatatt tttaaattca gaaggtgtaa tattagaatg
     6541 ttctatgagc aatattttct ttataaaagg agaaagagtg tacactccta gtgatagatt
     6601 accgatatta aatggaataa taaaaaaaag gattatagag atttgtgacg aattacatat
     6661 agagttgata gaaaatgaaa ttaatatatc agaaattagt agttttgatt ttgtttttgt
     6721 cacaaatagt cttatgggcg ctataaaggt tacagaaatt gacaaaataa aatttaataa
     6781 agaaaatgtt gtttttgata aaatagtgaa gttattagaa taaattaaaa tattaacaat
     6841 ttgttggaga caaaagatga taggttgtat ttatgaagta tacaatgaag agatgactaa
     6901 aataatagaa aaccaaaaca ctttggctgt atatttagta ggttctagta aagacgtaga
     6961 ttttactctt tatgatgtag agataaacga tattgatgtg ttcgtttttg taaaagaagg
     7021 ggaaaaacaa gagagaatac tgtttaaaaa aaagggaata gaatttgatg taaattactt
     7081 ttcaaaagat ggtgtaaaaa aattattatc aaatagggaa tatttctttg tgaaagaaat
     7141 gaaagatgct aaagtactgt ttgataggga gaatatatca aatattataa aagatataag
     7201 tagagatatt tacttagaag gacccagtaa aatgtcatta gaggaaaagt cttttataaa
     7261 gcaagatatt ggagcaaaaa tatcgaattt aaaaaataaa gaaaaatttg atgtttttga
     7321 atatcatttt ttgacgaatt tatatttaaa agatataata attgggtact ttaatataaa
     7381 tgataaatgg gtaccaaaag acaaaaaatt gctaaaagtt ttaaaaaaag aaaataatga
     7441 gctttttgaa ctagcgtcaa aagtaagtga aaattatgac tatcaaagac ttttagatgt
     7501 ttataattat atttttaaag aaattgagac aaaagaggtt ataaaactaa tcttctagtc
     7561 acaaatcaat ggggggagtt taaatgaata aagttgataa agaaaagata caacatgcag
     7621 taagagaaat actagaggca attggagaag acccagatag agaaggtcta atcgagactc
     7681 caaatagagt tgctaggatg tatgaagaga tattctctgg tcttagtgaa gaaccaagag
     7741 accatttaaa agttcttttt gcagatgaaa aacatgaaga attagtattg gtaaaagata
     7801 ttccatttta ttcatgttgt gagcatcatc tagttccttt ctttggaaaa gctcatattg
     7861 cttatttacc aaaaggagga agacttacag ggctttctaa acttgctaga gtaatagata
     7921 cattggcaaa aagaccacaa ttacaagaaa gaatcactaa aaatgctgca gatataataa
     7981 tggaagaatt acaaccttat ggtgtcttag tagtagtaga agctgaacat atgtgtatga
     8041 caatgagagg tgtaaaaaaa ccaggttcaa aaactgttac atcagcagtg agaggaatat
     8101 ttgaaaagga cattgcgtct agagccgagg ctatgagttt gattactatg aagtagcaat
     8161 ttattataga tataatcatt tattgagata taaaatttaa tttaagggag tgtcattaat
     8221 tgtgtttgat tatggtaaaa gaacttatat aatgggaata ttaaatgtaa ctccagatag
     8281 tttttctgat ggaggagatt ttaataactt ggacatagca atacaacatg caaaagatat
     8341 ggttgaccaa ggtgcagata taatagactt aggaggagag tctacacgcc caggtcatag
     8401 ctatgtagac tcagacgaag agctaagaag agtaatccct gtaataaaaa aacttaaaca
     8461 ggaattggag ataccaatat caatagacac ttataaggca gatgttgcag aagaagcatt
     8521 aaaattaggt gtcactatgg taaatgatgt ttggggtctt agaaaagata aaaatatggc
     8581 ttctgtaata ggaaaatatg atgcagaagt gtgtataatg cataatcaag atgggacaaa
     8641 ctatgacaaa gatataatgg agtcaataaa agactttttt aaggtaagta tagaaatggc
     8701 aatgagttgt ggtgtaaaaa aagaaaaaat agtacttgac ccaggcgtag gatttggcaa
     8761 ggattttgaa caaaatattg aagtattgag aagattaaat gaattaaaag atttagggta
     8821 tcctattttg cttgggacat ccagaaaatc agtaatagga aaagtacttc cagttgagcc
     8881 taaaaaaaga ttagaaggta caatagctac tactgttctt ggtataagag atggcgtaga
     8941 tatagttaga gtacatgatg tttatgaaaa tttaatggca gctagaatga ctgatgcaat
     9001 atatagaaag taggtataat ataatggata aaatattact tagtaatcta ggattctatg
     9061 gatatcatgg tgttcttaaa gaagaaaatt ttctaggtca gaaattcttt gtcgacatgg
     9121 aactttatat tgattcaaga gaagcaggtc ttagtgacga tataaataaa tctgtaagct
     9181 atgccgaggt ttacaatgtt gttaaagata ttactgaaaa taaacaattt aatcttttgg
     9241 aggctttagc ggaaaatatt gctgaagaag tcttgaataa atttatactt ataaatggag
     9301 ttatggtcag agttagaaag cctgaggctc ctgtaaatgg tatttatgac tattttggtg
     9361 ttgagataag gagaacaaga gatgaataag gcttatttgg gaataggtac taacatgggt
     9421 gatagatttg ataatttatc aagagcatgt gaacttttga aaaatagtga ttctatatat
     9481 aaagtaaagg aatcaagttt atatgaaaca aaaccatggg gatatacaga acaagcagat
     9541 tttctaaata tgtgtgtaga aatagaaaca gaatttgaac cttatgagtt gttagaatat
     9601 tgtcaagaaa tagagagaga actacacaga gagagaatag ttcattgggg accaagaact
     9661 atagatgtag acatactatt ttttaatgat gtagcttcca ctgatgaaag actaacaata
     9721 cctcatccga ggattcaaga tagagctttc gttttgatac cacttatgga tttaaatgaa
     9781 gaacttataa taaatgaaaa aacagtaaaa gaacatttaa acctcctatc tgctgaagaa
     9841 agagaagaag taaaggagct tgtaggttat gaaagaaaac ctatttaata ataattcaaa
     9901 gatagagata aatacaaata acaaaaagat aactgtaaaa aaagataaac ttataatagc
     9961 tggaccatgt gcaatagaga gttatgagca acttttggag actgctaaat ttgtaaaaag
    10021 tcagggagca aatattttaa gaggaggagc gtacaagcca agaacatcgc caaattcatt
    10081 tcaaggatta aaaaaagaag gtcttaaaat attaaaagca gttaaagacg aagttggtat
    10141 ggctgttatt acagaactta tggatgtaag agatatcgac gaactgtata gtataagtga
    10201 tataattcaa attggttcaa gaaatatgca aaactttact cttttaagtg aagtgggaaa
    10261 acaaaataaa cctgttatgt taaaaagagg aatagcatcc acaattactg aatggatagg
    10321 tgcttctgaa tatatagcaa tagaaggaaa tagtaacatt ataatgtgtg agagaggtat
    10381 aagaacgtac aatgattata ctagaaatac attagattta gctgcggtac ccattattca
    10441 aaaggaaaca ggtcttcctg ttgtggtaga cccaagtcat gccactggag ttagatattt
    10501 ggtaaaacct atgtcactgg catcttttgc atgtggagca gatggaataa tggtagaagt
    10561 acatcctgac ccagaaaatg ctttgtcaga tggtgctcaa tctctttgtt ttaatgaatt
    10621 tgaagattta atgaaatcaa ttaataatta ttaaaagagg caagagcctc ttttttattc
    10681 gtaatcattg ctaaggtaaa tttataagtt ataatttgat agttagatta tttaatataa
    10741 ataaagattt aatattgata gaattaatat aaaaaataaa aattttttga ttaataagaa
    10801 ggatatcaat aaaaaaagtc gaattgtgta tattgagtga ttgacaaatt tagaatatag
    10861 tgtagaatag aaatgttaac acaaaaaatt acaccagaag gtgataatat gaatgatata
    10921 aaagacatta ttgaagaaat aaaagctaga tgcgatatag caagtataat atctgaatat
    10981 atgaatataa aacaatctgg agctaattac aaagggttat gtccatttca tggtgaaaag
    11041 acaccttctt tttatataaa tacatcaaaa cagatttata agtgttttgg ttgtggtgaa
    11101 ggtggagacg taataaattt tgtcatgaaa atggaaaatt tggattttat ggatgctgta
    11161 aaaattttag caaataaatg cggtatagaa attaacacta acatgaatga agaaactagg
    11221 attaaaatag aaaaatctaa gaaatttcaa gatatccata cggaagcagc tagattttat
    11281 ctttcaaact tattgggaag caaaaatctt gggtatgaat atttaagaat aagaggacta
    11341 gatgataaaa ttattaagaa atttggttta ggattttctt tggattcatg gaattcactt
    11401 atgaatactc taataagtaa aggatacaaa aaacaagact tattggattg cggtctaatt
    11461 gcaaaaaata gagatggaac taattgctat gataaattta gaaatagagt tatgtttcct
    11521 atatttgact atagaggaaa cataatagga tttgggggaa gagtattaga tgattctttg
    11581 ccaaaatact taaattctcc agacacatta atttttaata aaaaacagaa cttatacgga
    11641 ttaaattttg ctagaaaaaa tttagaaagt aaaactatag tcttagttga aggatatatg
    11701 gatttaattt cgctttatca gtatggtata aaaaatgtag tagcaaccct tggaacagcc
    11761 ttgactgaac aacaaggcat tttaataaag agatatgcgg acactgctat tatatcttat
    11821 gattctgatg aagcaggaat aaaagcgact ttaagagcta tagatatact tactaaatta
    11881 ggtatcaatg taaaagtgtt agacttaaaa gatgctaaag acccagatga atttgtaaga
    11941 aaatacggac ttagtgatta taaaaaagct atggatgttt ctactcacta cataaaatat
    12001 aaaatagatc atcttaaaaa agaatttaac attcaaaaag atgaagaacg agtgaagttc
    12061 gcaaaagaag cttctaaaat tataaaacaa ttgacaagtc ctgttgaaat tgatttttat
    12121 acaaagtatt taagcaatca aatagatatt aatgtcgagt ctataaagag agaagtttat
    12181 ggcaaaaatt acaataagcc atataataac aaaaatcaaa agaaaataga agaaaaagta
    12241 attgaaaaag tcgaagtcag acaagacgga aaacaattag ttgaagaaac tctaataaaa
    12301 ataatgttag aagacaaaaa aattagagaa atagcgttac ttaaagtaga agaaagtgat
    12361 tttttattga aagaaagtaa agaaatttta aattacatga ttaaaaatca agaattggac
    12421 aaaataacta ttgacaaatt gaaaagttta aacatatccg aagaatactt aaaagaatta
    12481 aattcaattt ctttgaatag tataaactta gagaacacaa aagaaataga aggaataata
    12541 acaaatatca gaaaaaactc attagaagag caaattaata gcttgttgag agaacaacaa
    12601 gaattagaaa ataataatga tatgaaagag gtagatggta gagttatgga aattgcttta
    12661 aaaatagttg agataaataa aattttaaag agcttgtaga gaaaggaggt ttgagtgtgg
    12721 aaaataaatc gaataaaaaa gagttaaaaa aggttactgc taagacatta atagaaaaag
    12781 gaaaaaagca aggttctctg acacttgcag agataatgga agctttttcg gagactgaac
    12841 ttgataagga tcaagtagaa aatctttatg agactttagg aaatttggga atagaaataa
    12901 cagaaacaaa aaattataaa gctgatatag atttttcggt tgctgatgac gatttaagta
    12961 taggccactt agatgaagat gcagaggcaa tttcacatga tgactcttct gcaatagaaa
    13021 tagaaactgt ggatttatct ttaccaaaag ggataagtat agatgaccct gtaagaatgt
    13081 acttaaaaga aataggaaaa attcctctac ttaaaccaca tgaagaagta gaatttgcta
    13141 gaaggatgca cgaaggtgat gagatagcaa aacaaaggtt agttgaagca aacttaagac
    13201 tagttgtaag tatagcaaaa agatatgtag gaagaggtat gcttttcttg gatttaatac
    13261 aagagggaaa tttaggtctt ataaaagcag tcgaaaaatt tgactataca aaaggatata
    13321 agtttagtac ttatgcaaca tggtggataa gacaagctat aacacgtgct attgcagacc
    13381 aagctagaac tataagaata ccagttcata tggtagagac tataaataag ctaataagag
    13441 tatcaagaca actacttcaa gaacttggaa gagatccaaa accagaagaa attgcaaaag
    13501 aaatggaaat gacagaagat aaagtaagag aaataatgaa aatagctcaa gaccctgtgt
    13561 ctttagaaac accaatagga gaagaagaag atagccattt aggtgatttt attccagatg
    13621 atgatgctcc agccccagca gaggcagcag catattcttt attaaaagaa caaatagaag
    13681 atgtacttgg ttcgttaaat gatagagaac aaaaagtatt aaagcttaga tttggtcttg
    13741 aggatggtag agccagaact cttgaggaag ttggaaaaga gtttgatgta actagagaga
    13801 gaataagaca gatagaagca aaagcactaa gaaaactaag acatccaagt agatcaaaaa
    13861 aacttagaga ttatttagac taaaaagata catcattgat gtttttgtga taaatgccat
    13921 gagataagtg atatctcatg gctattttgt agtaaagatt taattttatt tttgtagtta
    13981 tttcagtcta atatctataa cttggattaa atcctataaa taaagggaat tgaaaggaga
    14041 attatatttg aaattaacag atagattatt gaaaatagca tcattagtgt cagatggtaa
    14101 aaaaatagct gatataggga cggaccacgg atacatacca gtatatttgt taaaagaggg
    14161 aaaagtacca tttgcagtgc tagcagatgt taataaagga cctcttgaca atgcacataa
    14221 agaagtcata cagaacaatc ttttagataa agttgattta cgattgggtt ctggtataga
    14281 gatacttgaa ataggggaag tagaagaagt gataattgct ggtatgggtg gaatcttgat
    14341 aagtgagctt ctcgaggcta aaaaagaagt agcacataat gtagaaaaat taatattaca
    14401 gccaatgcaa gcacaagaag aacttagata ttacctttta aacaacggat atgaaatttt
    14461 agaagaagtt ttagtgagag aagattttag aatatatgag ataattgttg ccaaatatac
    14521 aggtaaaaat actataatag aagatgagat atattatgag gttggaataa agcttttaga
    14581 aaacaaagat tctcttttta atgattttat agaaaaaaag ataaagacat atagttctat
    14641 agttaataaa ttagaaggga aaaatgggga agctatagat aagaagagaa aggaaagcga
    14701 agttgcaata aaaaaacttg aaaatttaat aaaataaata ggaggtatat atgctattaa
    14761 agagtctaac tagaaaaatt gaaaaaaagt atcctctaaa cttagcagag gattgggata
    14821 atgtaggtct tatagttgga gattttgata tggatgtaaa aaaggtattg gtatctttag
    14881 aagctaatga agatgttatt gatgaagcta tatccaaaaa tatagattta attgtgacac
    14941 atcatccttt tatatttggg aaaattaata aaattaatag tggagattta aaaggcagac
    15001 ttatccaaaa acttattaaa aatgatatat cactttattc tatgcatacg aattttgata
    15061 tagcatttga tggtctaaat gactatttta tggaaattat ggaatttggt aattcaaagg
    15121 ttttagatat aactaaaagc gaaaatttgt ataaattagc agtatacgtt ccacataact
    15181 attcagatga acttagaaag gttttaagta attcaggagc aggtcatata gggaattata
    15241 gtgattgtac gttttcaata gaaggtgaag gacaatttaa gccattagaa ggctctaatc
    15301 cgtttcttgg aagtgtaaat gatatagaaa ctgtaaatga agttaaaatt gaaactgtag
    15361 taccacagaa gcttttaggt ggggtaataa gttctatgtt agatgcacat ccttatgaag
    15421 aagttgcata tgatttgtac aagttagaaa ataaaggtga gatatttgga ctaggtagaa
    15481 taagtaaact tgataaaagt atgacattag aaagtctttc aaggaaaata aaagagaaat
    15541 taaatatgaa acacataaga gtagttggaa accttagtac agacataacg aaagtagccg
    15601 ttgtaacagg agctggttca gaatttgtaa aaaaagctaa gagacaaggt gcagaagtac
    15661 taattacggg tgatgtaaag tatcatgagg cacaagatgc acttgatatt ggaatgtgta
    15721 tagtggattg tggtcatttt gatacagaag atatattcaa aaatgttatg aagagatttt
    15781 tagatgaatt tagtgaaatt gaagtaataa agagtaatgt atatttgaat ccatttagta
    15841 ctatttagaa gtgtttgatt aaaaaatact atataaaaaa taaatttgag tttaattagg
    15901 gggataatga tgaaagtagc agtaattttt catagtgttt gtgggagtac atatttatta
    15961 gctagagaat ataaagaagt gcttgaagaa atgaatatcg aggtagatat atttagagta
    16021 agtgacgaag ttgcaaaaac acttcctcaa tattatttga taaattacaa agaatacaaa
    16081 gacgaatttg aaagtataaa tgtaattaaa tctggaaaag aaatattaga ttatgatgct
    16141 atatttatgg gttcaccaac atattatgga aatgtatctg gaccaatgaa gatgtttatg
    16201 gatagttttt ctgatatttg ggtaggtgct cctttgagtg gtaaaatatt tggttgtttt
    16261 gctacagcag gttcacaaca tggaggagga gaattagctt tgcaagctat gaatatattt
    16321 gctcaacata tgggaatgac tcttttgtca gttccatgtt cagtaagagg aggttatcca
    16381 gcgtatggaa tacttcatat agcaggagat aattctgata taagaccaaa tgatgatgcc
    16441 aagataggta taagagatta cctaaaaaga cttaacatat aagaatatca tataaaaata
    16501 cctaaaagta gctgtatata cttttaggta tttttactta attataaacg tgaagcttta
    16561 ccagaaatat ggtcaatttc tattttaaca acagttgtta agttcatatc cttctctatg
    16621 tacttcatgc cttcttcaaa gaatccttta gaatattttt ttattatttc tacaagagca
    16681 tttttctttt cttcattttc tacagtgcaa gcttttccaa aaactatagc actagaataa
    16741 gtagtactaa atttatctgg aataacactt tcattagcaa ctactaaaaa agaaactttg
    16801 ttgtttttcg atatattatc taacttatgt ccatttcttg cacaatgaaa gtaaatagaa
    16861 tcattaaagt atacataatt tacagcaact ccataaggat aaccattttc agatatagtg
    16921 gaaaaagtac caaattcacc attttttaat acttcaacag tatcttcttt tgtcatttct
    16981 ctttttttca atctcatttc tttaaacata atatcatcct ttctaatttt gatgcttaaa
    17041 ttaaatttta gaacatttat aaagattatg ctatgttttt tacaaatttt aagaaaaaaa
    17101 tatagtttaa gataaaaggt agtgaaagag aagctatttg gaggtttatt tatgatatta
    17161 ttgttagagt agtacaaaca atactaataa atttaatagc tggtttcttg ttatttaagg
    17221 tagtaagcat gtctataaga aggggaagtt atcgagggta gaagttgtta tttataacga
    17281 tattataaag aaaatattat tatataaaat aataaatagt aaagaggtat caagttgaat
    17341 ataatactta atacctcttc aaaattaaat tataaaagtg ttgtgggaat tgtcctatac
    17401 atggtaagga gataataaag aatgtatctt taaagtctca actaaacagc aaaatttatc
    17461 caacaaacat acaataaatg cttctttgta tctaggtggc ttaacagtaa gtggccacat
    17521 gtgttttaaa attatgtccg tttctttttc atttatttga aaaaataaag ttgcattttt
    17581 taatgcttct ctagggtgag taaatccatg taatcctttt ctatcacctt tgtaatgcca
    17641 atcataaaga aaaaagtcgt gtagtaaagc tcctctaata acactttgaa tatcaacacg
    17701 taagtgcaat tttttacata atagataact ataatatgct actgacaaac tatgttcaag
    17761 acatgaaaca ttgccatgat gaggaaatgt tctcataagt tgaacttttt cagaagaaag
    17821 tatctcagaa gcacattcat taaaatattt gatttcagct tttgacaaca ttatgaaaat
    17881 ctccattctt agtaatatat aaatactata cttcaacgat aatatgaaat atttcttatc
    17941 ctacaaaata taatacccaa taatacaagt aagatacctt ttatggtatt ttaaagtaag
    18001 agagatttta ttaggctaaa caatgaaaaa attaaaagta aagctatagc aatatatagt
    18061 atttttccaa gaggattgcc aaaatttata gtccatccaa taccaaacct tttattaacc
    18121 attaatgatg ggtcatttgg attattatac atgatgcctg ctatccaata cttctcatct
    18181 tcttctggag aataagatga gttgaatttt aaatttgggg aattaatata tgttgctatt
    18241 aaataaatta taactaaaag cgttgtaata ttcatagcag ttgatagttt tgtttttatt
    18301 gataaaagag tagtaaaaaa ctgtgttgtc attataatca tcaatattaa aaacaaataa
    18361 ccaattttat ttaaatattt aatgttttca actctacttt cttcaattct atttgtatct
    18421 atcttaatcc ttgactttat ggatccaatg gaagttatat ataataaaac cattaaacaa
    18481 aagtcagtac cgattaattt aaatacatta agaaatgatt tatctataaa aacatcagga
    18541 gacccattta tgttccaatg agtggcaatt gagtctggca atttattata attaaatgct
    18601 ataaaaatac tcatccccaa agtaaatagt atcggtatta aatagattat tttaaatttt
    18661 tttattattt tatttttctt atttataaaa tccatatcta ctattaattt tgaatctaca
    18721 tctatatgac ctaatttaga ataaatttct gatttaagct tcttagcttt tttatgtgta
    18781 tgaatatata gtatactctc atataataaa aaacctataa taccaataaa atatgaaagt
    18841 tccaatttat taaatgtaaa aaccagaata aaaactaata taaagtctat aataaaacct
    18901 aaagataata gttttttaaa ttctttatct aattttataa agtaattata atttttatgc
    18961 tcttggtcta tagaaactcc atagatgtgt tttttaccta caaaataggg cataaaataa
    19021 aatgtagcat acatcaaaat tagaactgga aatattgtaa atagacattc aatcttactc
    19081 ataatatttc acctcatact tgtcaaataa acttgaactt agtgacaaaa attcttcttt
    19141 actaatacct gataaataag attttgccac caataattcc aagtcaagtt tatttttttg
    19201 acgagttgta ttatctattt tcattggtag ttgagctaca atagcacctt ttcttctatc
    19261 aatatttaag aatccctctt cttttaactc attgtaggat ttgttcactg tatgtaaatt
    19321 tacaccaata ttttctgcca tacttcttac agatggaagt gattcattta ttttcaattc
    19381 tcctgaagct atagccttag ttatttcttc tttaatttgt acataaatag atttatcact
    19441 attaaaatct aaatttaaaa ccataaaaaa cctccattat gctttataaa tgaaaacgat
    19501 tgttatatac atagtataac ataaaaaata ataacaaaca caaaatataa gtttatattt
    19561 tgtaaaaatt gtaatttaaa aaataacata atttttatta aaataataca gatactattt
    19621 atagatagaa ataaaaatta gaaaggagaa aaaatatgtt aacatattta atgaaaagac
    19681 aaatgcataa aaaagatgat ggaatgagta aaccaatgat gattacagga gctgtaatta
    19741 caggtgtagg agcatatgct gtatacaaaa tggtaaaaag ccataaatct aattctcaaa
    19801 tggtcaatac aaattattat ggaaatagcg attatgatga ttataaatat gacgaatttg
    19861 attatgactg tgattgtaga gacacaaaac aagattatga atatgaagaa ttaaaaaaaa
    19921 aagtaaaaga atttaattca agaagaataa attgtgaaaa ttgtccacat gtttctcaag
    19981 aagaaatgat gcaatatgta aatagtgtta aagatgtaaa ggaagatatt gatttacatg
    20041 aagatgctaa aaaagataat atttacaaag aagatgaata caaaaaatat gaagaagaat
    20101 aagtaatatt ttgtataact gaacaaatag taatgtcaaa ttatttaata tctagtttta
    20161 atatgtaagt tgtaacttaa cttgaaagaa tcagtatcat tctttagata gttaagttac
    20221 aacttacatt ttgtttttat aaattttaat agttctttgt gactattttg taattttaga
    20281 aatattttag tatccagggt aattgtgtat aataaaactt gtatagatag atagtatgaa
    20341 attaagcaga tagtttaaaa ttaaaatgaa aagaaaggaa atagttatgt taaagttgtt
    20401 gatagttgaa gatgatagta ctatatcttt tggaataaag tatgctttag aacaagaagg
    20461 atttagtata gatatatcaa aggatttatc tagtggaaaa gaaatgattt cttctaatga
    20521 atatagcatg atactgttgg atgttacatt gccagatgga actggttatg agctttgtaa
    20581 atatataaga ggattctctc aggttcctat aatattcttg actgcttgtg atgaagaagt
    20641 aaatattgta atgggtctcg atataggtgg agatgattat ataacaaaac cttttagaat
    20701 tagagaatta atatccagaa taaaagctgt tttaagaaga aaaggtaata cttcagaaga
    20761 aaataagaaa atacttaaat ttggagattt aagtatatat acattagaag ctagagtata
    20821 taaaaatgat aaagaaatat ttctgacatc agttgaatat aaacttcttt taattcttat
    20881 acagaataaa aatactgttt taagtagaac tcaaatattg gaaaaacttt gggatgtaac
    20941 ttatgatttt gtaaacgaca atactctaac tgtttatata aaaaggctta gagaaaaaat
    21001 aggtgatgat ttatgtgaac caatctacat agaaactgta agggggattg gttataaatg
    21061 gataggaagt gaaaatagtg tttctatata atccagaagt gaagaagttt ttaagtaagt
    21121 atgttacttt gatgtttatt gttatcataa tatctattgg atttagcata atcaacgtgt
    21181 ctttaactaa agagatggtt gtaagaaata atcaagctat aatagggact ttatcgagta
    21241 agtatccaaa cttagaaagt cagatagtag acataataac tcaagggaag tctatgaaga
    21301 acatagatta tggtcagaaa atattaagta agtataatta tgataaatct attaagataa
    21361 actctgagcc tattatttct aaattagttt tagagactat caaaataaat ataatattag
    21421 tatgtataat atttattttg atatttgtgt tggttgttag atattttacg tcaatttaca
    21481 atgatttatc agatatgact aagtatgttt actatagttc agagggtaag ccatttgata
    21541 tgaaaaacaa gaatcaagaa gggcagatag gtcttctaaa aactgaactc ttaaagatga
    21601 cgacaattct taatgaaaag gttgaacttt taaaaagaga aaagatattt ttaaacaata
    21661 caatttccga tatatcacat caattaaaaa ctcctatgac ttctttaata atgttgaatg
    21721 atttattata taatgatgta ccatacgaag taaaaatcga ttttttaaat aagataaaaa
    21781 atcaattaaa tagaatggat tggttgatta aaagcatgct taaattatca aaggtagaag
    21841 caaaagtgat aaattttaaa aaagataagg taaaatttag tgaactcata catagagcaa
    21901 tgctatctat gaaaatacct atggagatta aaaatcaaaa actgactata gagggaaatg
    21961 ataatgtatc ttatattggt gatattgatt ggtctgtgga agcactagtg aatataatca
    22021 aaaattgtgt agaacatact cctgaatttg gaaatataat tataacctat aaagaaaatc
    22081 cattattttc tgaactaata ataaaagatg agggtgaagg tatagataaa aaagatatac
    22141 cacatgtatt taaacgattc tatagaggaa gaagtagttc aaaagaagat agtgtaggaa
    22201 taggtcttgc aatgtcaaaa tctataatag aaagccaaaa tggcgatata tatgtaaaca
    22261 gcgaaaaagg taagggtaca gaatttcata taatatttca taaaatgtat gatagtgact
    22321 aatctgtaat atttaattca ctttatagta atagccaaaa cctaatatat gaagtataga
    22381 atataaatat tggaggtagt tatggaaatt ttaagagtag aaaatctaac taaaagttat
    22441 ggtaaaaatg aaacaaaagt tgatgctata aaaaatgtaa gtttatcagt tgaaaaagga
    22501 acgtttatag caataacagg tccaagtgga agtggtaaaa gtaccttatt acatttatta
    22561 ggaggagttg acagaccaac tagtggtaag gtttatataa atgatgttga tatatataat
    22621 ttaaaaacta aggatttggc gatatttaga agaagaaata taggtcttat atatcaattt
    22681 tacaacttaa ttccagtctt aaatgtgaag gaaaacatat tgcttccagc agagcttgac
    22741 aatagagata ttgatagaga atattttgat gatttaatga aaacattagg tctaattgat
    22801 agagaaaagc accttccaaa ccaattatca ggaggtcaac aacagagaac ctctatagga
    22861 cgtgcattaa ttaatagacc ttctataata ttagcagatg agccaacagg taatttagat
    22921 agtaaaaatt caaaggaaat attagaattg ttaaaattgt cagtaaaaaa atacaatcag
    22981 acactaataa tgataactca tgacaaaaat atggcacttc aagctgatag aattataagc
    23041 atagaagatg gaatcataaa aagtgatgag gtgatgtaga tgaatttata tacatcacta
    23101 accattagat acttaaaaca aaataaaaga agaaccattg taaccataat cggtataata
    23161 ctttcaacag ctttaatttg tggtatagga aatatatttg aaagttttat ggattatcaa
    23221 atgagagaaa ctataaagaa tgatggaagt ttccatgtta cattttatga tgtaaataga
    23281 aaaaatgtgg aatatgtaac gaaatcagct gaaatagaaa aacatgcatt taccaaacaa
    23341 cttggatatt ctaaattaga aaatagtgaa aatgctatat taagtataaa gcaatatgat
    23401 caaaatgcaa taaacggata taaagtttct ataaaagaag gtagatttcc agctaaggaa
    23461 ggagaaattg ttttaagtga aagtattatt aatcttatag atgataagtt aaaaatagga
    23521 gataagataa ctttaaaagt aggggatatg tttgactcaa gcaagaaaaa gattgatagt
    23581 atgacatttc atgaaggtga ttatatagca aatgaaaaag atagaacttt taaagttgtt
    23641 ggtataatta aaaaacctgg gtttgaacgt tataatggaa ttgcaacagc tatagcctac
    23701 tttaatccta tgaataatta caatgataca attaatgtat cagtagcagt aagaaatcct
    23761 aaagatgtgt ataagataag taataaaata agtaaaaata tatattcaaa taaagataaa
    23821 gaagcagttt ctgatatgat aaaatacaat gaacatttat tgaggttaca aggtgctagt
    23881 aagtattcaa atataaatag ctctataaag tctataatta tagcagtaac tattttagta
    23941 atcatatgta caattgctac agtgtacaat tcctttagta tttctataaa tgaacgaaaa
    24001 aaacagtttg ggatattaaa ttctataggt gctacatcta gtcaaattaa aagattagtt
    24061 tttatagaag gtattataat tagtttaatt ggtataccaa taggactaat atctggaaca
    24121 gtagctatag acctattgtt taaaattata aataagtatt ttactgaatc ggttgttact
    24181 aagatgagtt tgcagatagt gtataatcca ataataatta ttgttagtat aataatagtt
    24241 ttgcttacaa tctttatatc aattctacta cctgcaatat cggcttctaa tatttctccc
    24301 cttaatgtga ttaaaaatac tggagagtat aaagtgggca aaatcaaaag ctcaagactt
    24361 attaaaatga tttttaaaac tgaaggagta cttgcatata aaaatattag gagaaataga
    24421 aaaaaattta taataactct tttttcttta atggtaagta taataatatt tatatctttt
    24481 agtggcttta cattactttt actaagaggt gaagaaatta gaaattctca aagaaaatat
    24541 gatttgtatt taactgcaaa gaacacagct aggtctgtag aggatactat aagtgaacta
    24601 gaagatattg atgggataaa aaactttgaa ttggcaacag gacaatatgt gtctataaga
    24661 gtaagtgaaa ataaaataaa taaatccaag gaagatttaa tcaggaaata ttatcaaaaa
    24721 tataagattg gagaaagtta tgaatatgat ttcagtaata atgaattgat ttttccggga
    24781 gattttgcag taaaaaacaa aataaataac ttagtacagg gctcttttaa taaagagaga
    24841 gcaattgaag aaaatggtgt tatattagtt agaaaaagtg caattgaaga aaatgggaag
    24901 aaaggtgtag tagaacttac caactataaa gtaggagata cagtaaattg tgaatatctt
    24961 gatgaaaatg ccttaagtaa aaaagtaaag gtcaagattc ttgctataac tgatgaagaa
    25021 cgtctaggtt ttggttatca aaatatgggg ttacaattta taacttatga tgaagttgct
    25081 aaaaatttaa atttaaaatt aagtaaaagc cttatattta ttgattcagg aggaagcatt
    25141 aagactaaga aggaaataga agctttggca aataaaaatg attttaactt ttatgatgaa
    25201 atttcaagca gtgaaagtga aaaacaagat ttaaaggtta ttaaaatatt tgtatatggt
    25261 tttatagtag tgatatcttt agtgagtgtt acaaatattc taaatacagt aagtacaagt
    25321 ataaatctaa gaaaaagaga acttgcagta atacaatcta taggggttac tcctaaagga
    25381 tttaggaaaa tgatatattt agaaagtttt atatatggta tactgtcatt gttgtttgga
    25441 ataccaatta gtattggtat aattctaatt atgaataaac ttatttcagg tgtaattgaa
    25501 ttttctccga ttataccgtg gacagccata gttatctgta tagtaagtgt ttttataata
    25561 acttttatag cagggtatat accaatgagt aaattaaata aagaaaatat aatagataat
    25621 ataagaagag aaagtatata gatataaaaa taa
//
LOCUS       DAGQWR010000053        25397 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.053, whole genome shotgun sequence.
ACCESSION   DAGQWR010000053 DAGQWR010000000
VERSION     DAGQWR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25397
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.053"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            163..837
                     /locus_tag="MIW95_001845"
     CDS             163..837
                     /locus_tag="MIW95_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3151173.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDSLKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            825..1985
                     /locus_tag="MIW95_001846"
     CDS             825..1985
                     /locus_tag="MIW95_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY3151174.1"
                     /translation="MDKISNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNINMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNPHFGMGLHISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2202..2729
                     /locus_tag="MIW95_001847"
     CDS             2202..2729
                     /locus_tag="MIW95_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBY3151175.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            3015..3683
                     /locus_tag="MIW95_001848"
     CDS             3015..3683
                     /locus_tag="MIW95_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151176.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLKINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3686..6154
                     /locus_tag="MIW95_001849"
     CDS             3686..6154
                     /locus_tag="MIW95_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151177.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASRVSPKIAMAGSTTKIKRKIKREKAIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYNSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNISLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6394..7818)
                     /locus_tag="MIW95_001850"
     CDS             complement(6394..7818)
                     /locus_tag="MIW95_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3151178.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMPYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANSRGR
                     FVFAVIIMALFSPFIYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMKGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7837..8529)
                     /locus_tag="MIW95_001851"
     CDS             complement(7837..8529)
                     /locus_tag="MIW95_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3151179.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8584..9360)
                     /locus_tag="MIW95_001852"
     CDS             complement(8584..9360)
                     /locus_tag="MIW95_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBY3151180.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(9357..10127)
                     /locus_tag="MIW95_001853"
     CDS             complement(9357..10127)
                     /locus_tag="MIW95_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBY3151181.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10124..10828)
                     /locus_tag="MIW95_001854"
     CDS             complement(10124..10828)
                     /locus_tag="MIW95_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBY3151182.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(11083..11595)
                     /locus_tag="MIW95_001855"
     CDS             complement(11083..11595)
                     /locus_tag="MIW95_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151183.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEETYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDISNYYLVANYI
                     RCLKKYKLID"
     gene            11921..12178
                     /locus_tag="MIW95_001856"
     CDS             11921..12178
                     /locus_tag="MIW95_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151184.1"
                     /translation="MILNRTELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FSNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12321..12947
                     /locus_tag="MIW95_001857"
     CDS             12321..12947
                     /locus_tag="MIW95_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBY3151185.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDNRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIEVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13197..13976)
                     /locus_tag="MIW95_001858"
     CDS             complement(13197..13976)
                     /locus_tag="MIW95_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBY3151186.1"
                     /translation="MNSKIYYFSGTGNSLVVSKILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIIKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPSDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(14044..14505)
                     /locus_tag="MIW95_001859"
     CDS             complement(14044..14505)
                     /locus_tag="MIW95_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY3151187.1"
                     /translation="MGGAGDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14481..15158)
                     /locus_tag="MIW95_001860"
     CDS             complement(14481..15158)
                     /locus_tag="MIW95_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY3151188.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15391..15777
                     /locus_tag="MIW95_001861"
     CDS             15391..15777
                     /locus_tag="MIW95_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY3151189.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15780..17579
                     /locus_tag="MIW95_001862"
     CDS             15780..17579
                     /locus_tag="MIW95_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBY3151190.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVIILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSIIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLKQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(17934..19808)
                     /locus_tag="MIW95_001863"
     CDS             complement(17934..19808)
                     /locus_tag="MIW95_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY3151191.1"
                     /translation="MNKYNKIANELIEIIGENNIISITHCATRLRVMVKDREIINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(19813..21477)
                     /locus_tag="MIW95_001864"
     CDS             complement(19813..21477)
                     /locus_tag="MIW95_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBY3151192.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDATPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSKYSDCLIYGSIEPIDIESDNVIAYRRQLDLVVIDCY
                     YNFSDKLVEIKTEEKYDIVFQNLDDINLLNKTVSLKPFQAVLLKVR"
     gene            complement(21491..22312)
                     /locus_tag="MIW95_001865"
     CDS             complement(21491..22312)
                     /locus_tag="MIW95_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBY3151193.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSIGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDVHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22411..23175)
                     /locus_tag="MIW95_001866"
     CDS             complement(22411..23175)
                     /locus_tag="MIW95_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY3151194.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVVKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDTSTIIDFAKEMRMKGTYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23172..23921)
                     /locus_tag="MIW95_001867"
     CDS             complement(23172..23921)
                     /locus_tag="MIW95_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY3151195.1"
                     /translation="MRLESLVNQSYNSLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKSGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(24425..25030)
                     /locus_tag="MIW95_001868"
     CDS             complement(24425..25030)
                     /locus_tag="MIW95_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY3151196.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMKIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            25209..25307
                     /locus_tag="MIW95_001869"
     CDS             25209..25307
                     /locus_tag="MIW95_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBY3151197.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFD"
BASE COUNT         9107 a         4094 c         3100 g         9096 t
ORIGIN      
        1 ttaaaatttc taataatttc atccttgatt tatttaatat ataaaaatat actaacttta
       61 atgattaagt tttgaaatat aactaagaat ttgagaattt attgagattt ttatactaaa
      121 atataaatac tatacaaact taatttagaa gggagcattc ttatgaacaa taaaatactc
      181 gtcgtagatg atgaggaagg tataataaca ctcttaaaag attactttga aatgaatggt
      241 tatgaggttt acactgctca aagtggaaaa gactcattaa aaaaaatatc caaaaatcca
      301 gatatcattc tacttgatat aaacatgcct gaaatagatg gaattgaagt ttgtaataga
      361 attagagatt atgtatcatg ccctattcta tttcttacag ccagaattga aaatactgac
      421 aagataaatg gttttcgtgc tggtgctgat gattatattg taaaaccttt tgatttagac
      481 gaacttggag ctagagtatc tgcacattta agacgtgaac aaaggaagtc aaatcaaact
      541 aaaattaaat tttataatga tttaacaata gattacgaaa aaaggttaat ttgtatatat
      601 aataatcaga tttcactttc aaaaaaggaa tttgatatag tagagcttct ctctatgaat
      661 cctggacagg tatttgataa agaaaaaata tatgatagag tctggggata caatagcaat
      721 ggaaatagtg atgttataat ggaacacatt agaaaaatac gaatcaaact atcaaaacat
      781 actcttgaaa attatataga aacagtctgg ggagttggtt ataaatggat aaaataagta
      841 atatgtcatt aaaaaaagct ttctttgttc ttactttatc ttcactaatt atagcatcag
      901 tacttactac tttcgcatac attttactta ataatctata taattctata caagataagt
      961 atttaaaaac acctataaat acaggtatta taaccatagt tcaaaacaat gtgaaccaat
     1021 ttagtgatta tgataaaaca ttacttaata taatcaacat acttcagctt gtacttccac
     1081 ttatattttt tataggactt ttattattag ctgatataat tttttacaag gtaaaattaa
     1141 aaacacccat agaaatcctc aataaaggtg caatggaaat atccaacaac aatttagatt
     1201 tttgcttaga atataataat aatgatgaat taggtaatct atgtaatgct tttgaaaaaa
     1261 tgaggtctga actaaataaa aacaacataa atatgtggac tatgattaat gatagaaaac
     1321 aacttaatgc tgctttttct catgatttaa gaaatcccct aacagttcta aaaggttact
     1381 caaactattt aacaaaatac ataccaacag gcaaacttag tgatgagaag attttatcaa
     1441 ccacacaact tatgtctgag catattaaca gaattgaata ctatgtagaa aatatgagta
     1501 atgctcaacg attagaagat ttagttgttt caaaatctat gtcgaatata aacaagttta
     1561 tagaaaactt agatgaaaat atatctatca ttgctaaaca agaaggaaaa tcttttacac
     1621 ttaaaagtca aataaataat ataaatctat tttttgatga aaatatcatt cttagagtcg
     1681 tcgaaaatat aatatcgaat gcttttagat atgctagaaa taatgtatcc atacttatat
     1741 atcttgaaca agaactcctt acctttgtaa ttgaagatga tggtatagga tttagtaagg
     1801 aatctctaaa attagcttta agacctttct atagagataa gactttaaat gacagcaatc
     1861 cacactttgg tatgggtctt catatctcaa aaatattatg tgaaaaacat ggtggctcta
     1921 tttctattga aaataattct actaacagtg ctaaaattac tgctaaattc tcaacaagaa
     1981 attaaaatga ttttgtaagc tttcattgtt cttatataag ctttgttagt ttagaattta
     2041 taaaatgtat ttcaatttac aaccaatata tatactattt tgtagaagtt gtaagagttg
     2101 cttcaattat aatattttgg caaaaggcat ttgaaacaat aatagaaata tatatttaaa
     2161 tatgtagaat agtatgaaac tagaattttg gaggtgatta tattaaaagt tttgctaata
     2221 atattacatt ctctcgtatt gtgtttgcat tgctaatgct tagttctaca ttctgttcac
     2281 tgcctttctg gatatgttat ttttggtgtg gaattagtga tatacttgat ggtcttattg
     2341 cccgaaagtt gagacaacaa agtggaatag gtgcaaagtt agatagtatt gctgatttca
     2401 tttttgcggt tacaattttg gttactgtcg gaaaaaatat tgctcttccc atgtatctat
     2461 ggtggtgtat tgttgcaata gcattattac ggttgattag ttacagtatt ggttttttta
     2521 agtatcgcac attttcatcg cttcatactt tgatgaataa aatcactgga atttctatct
     2581 ttgcttttcc attactctat atttgctttg gtcttgatgt tgcaggaatt atgatatgta
     2641 tcgtggcttt tctttcttct tttgaagaaa tagcaattat gataaaatca aaagaattaa
     2701 atcgtgatat aaaaagtata tttatgtgac aacttagaat ttgtgacaaa aataatttat
     2761 ataataatga ggaagtaaaa cataaaaaat tatccacaag tttatgtgta aaatagtttt
     2821 aaagcaatag agttgtattg taaagccttt agtgcataaa ttacatttta aattaaaaat
     2881 gatactgaaa ctgcttatca gcactgcgaa ttatccgtaa atagtgaaga ctttttagat
     2941 ttaagctaaa agttgggaaa aaattgagtt ttaaaactta cactcttctt atatagaaaa
     3001 ggagagtgtt ttttatgatt ataaaagcaa aacaattatc aaaaatatat ggttctaata
     3061 acaataaagt tatagcactt aataatgtaa atttaaaaat aaactcaggt gaatttgttt
     3121 ctgtaattgg accttctgga agtggaaaaa gcacactttt acatatttta agtggattag
     3181 ataatcctac ctctggacaa gtgttacttg atgataaaga tatatataaa cacactgaaa
     3241 aagaactatc tgcacttaga agaaaatcct ttggattcgt ctttcaacaa tttaatcttt
     3301 tacctgttct tacagcatct gaaaatattt caatgcctgt acttctagat aaaaaacaac
     3361 ctgataaaga ctatttaaat gaaatttcat cattacttgg aattgcagat agactcaatc
     3421 atcttcccca tgaactttca ggcggtcagc aacaaagagt tgcaatcgct agagcactta
     3481 ttgcaaaacc agatataata tttgctgatg agcctacagg aaacttggac agtaaaagtg
     3541 gcagtgaagt tatgaatctt cttataaaaa cttcaaaaca gtttggaaag actttagtgg
     3601 taattactca tgatgataga attgcaaaac ttgctgatag aaaaatttca attattgatg
     3661 gtgttcttat ggaggtgaaa tagaaatgaa gaattacctt tcactttcaa taaaagaatt
     3721 aaaaagccag aaacttatga ctacattcat cattatagca atagtactat caactattat
     3781 gacaactgtg gttggacagt caattggtat cctacaaaat ttaagaattg aacaagcccg
     3841 ttcttttaat ggtgatagac atgtatcctt tcaccaactt actaaaaatc aagttgatga
     3901 cataaagaaa gatgatagag tttatcaagc tggtacttca ataactattg gcagtagtaa
     3961 aataaaggat tctggtatct ctgtacttgt aaaagagtat gataaaacag gactttctaa
     4021 ctacccaaaa ttaatgaaat taaaatctgg acatttgcct aaagataaaa acgaaatagc
     4081 cttagatgaa aacactctaa agcttatggg tataaaacca agactaggag tgactatacc
     4141 tatgaattta gatatttcac ttttaaatga taccatacca ccatataatt acacagctaa
     4201 ctttaaattg agtggcattt tagaggatga ttatacagga tatgtaagtg gtatagtaaa
     4261 tggtattgta ggtaaaggta catcagaaaa tctattacct aagagatata ttttatcctc
     4321 tttggatttt aaaataaaac agcaagaaaa gtttcaagaa atagtaaatc aattagctaa
     4381 aaaaataaat ctttctcaca attcaattca atataattgg atttacttaa atgctcttaa
     4441 gatacagttt gaaaaagatg aaaatagttc taatagtgat ggtatatcta tgattatatt
     4501 ggtatcttta tttgttgcct tattggtttt actggcttct ggacttgtaa tctataatat
     4561 tttaaaaatc tctgttacaa agaaaattaa agagtatgga tgtttaagag caataggagc
     4621 tgaaccaaac cagatatata agatagttat tttacaaatc ttaattttat gtactgttgc
     4681 catacctatt ggagctgtaa ttggaattat ctcttctaaa ggcataactg gaatggtcac
     4741 taatatctta aatccagata tacttctagc aaatgataat aaagaaatta ctgagcttat
     4801 acataaaaat acaacagcat atatgttccc attagtatta agcactaatg tatctttgat
     4861 tttttcattt atttcagcat taccatcagc aatatatgct tcacgtgtct ctcccaaaat
     4921 tgctatggct ggtagtacta ccaaaatcaa aagaaaaatt aaaagagaaa aagcaataaa
     4981 aaactttgaa agacatctag catggctaaa cttaaaaaga aataaaggaa gaactataat
     5041 aactatacta tcattattta tgagtataac agtattcgtt gcattgagtg agttctctaa
     5101 tgtattagat gtatctaggt cagtaagcaa tttaaaagaa ggagattttt ccttaacaaa
     5161 tgaaatatct ggttttgata aatcaacact agataaaata gataaaatga aaaacgtcaa
     5221 tcgcacttcc tttataaagt actctgaata caaacagggt gaaatagata cagatataaa
     5281 ttttgaaaat agtggtgaga tgttaaaaat cattggaatt gacgagcaga ctttaaaaga
     5341 cttaatgcct tctattacag attctatact agaagacttt aaaaatggtt ctatctgttt
     5401 tataaaaaat ccgattgcta tttctacacc aggtgtaaaa actagacata caaatttaaa
     5461 acctaaagat agtatcacaa tcaacaaaaa acaactagat atatatagta cagtagataa
     5521 aatgttcttt ttacaaggta atggctggat aaatggtgtt gatgttattg tatataattc
     5581 tgtttataac accttgacaa ataaaaacaa gttcaaccag ataaatattt atgctaaaga
     5641 taaatcaaaa ttagaacaaa taagattgtc tattgaacag atttgtgaaa ataatcctgg
     5701 ctctcattgg atatcatata ttgaatcaga taagcaactt aaagagagtt ttaaacaaat
     5761 agaatttcta gcatgggcag ttatattatt tgtgggattg attggtactt taaacatcat
     5821 taacacaact catacaaata taaatactag gactaatgag attggagtta aaagagctat
     5881 tggtatgagt aatattagct tatataaaat gtttttatgg gaaggtgttt attatggaat
     5941 ttttgctgct atatttggtt caattgctgg gtatgccagt gctattatta taaacatggc
     6001 aactatagaa aagcttgatt tcactaatat accaattact tcaattttac aagctactat
     6061 tatttctgta cttgcatgta ttatagctac tcttattccg ttaagaaaag ttaaaaagat
     6121 gaatataatt gactgtatag atatcaattt ataactattt tacagataat aatataaatt
     6181 aaattatcaa tgactgtctc tattttcttt caaatctaac taataaatct ttttaactat
     6241 aatataagct ttaaaaaagg cctgtctcaa aatagagata agcctttttt cattgtcgga
     6301 ataatgaaaa tatatttaac ttcacaaaaa aagagtgcct cacgaattaa aaatttcgct
     6361 ttgagacact cccctttaat ttaaaatata tagttaaatg ctatgccaaa atttcacaca
     6421 tgctccacct gattttgaat tttctatttt aacagaacct ccaagctgtt ccactaattg
     6481 tttgacaatg taaagcccaa gaccagaatg tccacctttt gtctgcctag cttcatctcc
     6541 tcgataaaac ttatcaagag ccttttttaa atctttagag ctaaaccctc taccagaatc
     6601 acaaatctca taagaaatac aatttttttc atcctttaca gtaatactaa tattaccacc
     6661 tgatggagtg tattgcagac tgttggatat gatattatca aaaattcgtg tcaacctatc
     6721 cacgtcgatt tgaattgggg attgtatatt ccccttcatt ttcaatataa gttcaacttc
     6781 cttttgatga gcctgtaatt catagtcttg caccttttgc ctcaaaaatt caggtaaatt
     6841 tataggaaca agatttaatt gtatatttga cttttctaag tcagacgtat attgcatctt
     6901 ttggacaaga gcagcacttt tttctgtatt ttctcttatc actgtaagat acctatgaag
     6961 tttttcattg tcatctgtat tattaccaat aagtgcatct gtatatccca aaataataga
     7021 aagaggagat tttaaatcat gtgctagagc ttccaccatt tcaacacgtt cctgctccat
     7081 cttccactgc gcagataaag aaccttttaa ttcgtctttc atttcagaaa aggcactaca
     7141 aagtttaccg agttcattat ctgaataata gtcgatttca aaatcaaggt ccttttcttt
     7201 tatcttcttg gacgcatcaa tcaatagatg taatggttga ttgatattct ttacaaagcg
     7261 cttagaaagc catctagtaa aacccacgat atatataaat ggagaaaaca gagccattat
     7321 aataactgca aatacaaatc tccctctact atttgcaaag gttattttga cttgatagaa
     7381 tagaataaca gctccctcaa tctttccatt atcccctttg attggaacag tatggatata
     7441 accaccttta cgaacagttt tattgataaa attattaaat aattcttctt ttgttttaaa
     7501 tagttttttg gggttagtac catatagaat attgccatta ttatccacaa cttgatatgg
     7561 catatcatcc ccacgaatcg ttctttttaa accttcttca ttcgattgtg ataacaaagt
     7621 tatgtttttt tcattgatgt atttttcaat tctaggaact tgtcgctcat aatagtttgg
     7681 tggatagata tccttattta aggaatgaat atacaaatag agagcaaata cataagtaat
     7741 cactgtggca actatactgg ctattataat aaaaataaaa gtatgtctaa attgggtaat
     7801 aagggattga tttttcattt aataacctct ctctctttac attttattcc acttataccc
     7861 aataccccaa actgtggaaa tatattcttt ttctggtgtc acagcagaaa acttagctcg
     7921 tatttttttt actcgttcaa caacagttga ggaatcacct tcagaatcac acccccatac
     7981 tttttcatag atttgttctc ttgagaaaac ttgtcctgca tggagtgcta gtaattcaac
     8041 aatatcatac tctatttttg ttaaatcaat attcttacca tcaattttaa ttgcacgttc
     8101 cttcatatat aagcataact tcccaaaata caaagtagat ctcttgttct cttcattgat
     8161 atactgtgaa cgcttttccc tacgcagatt agcttcaatc cttgccataa gttcacgcag
     8221 tccaaatggc tttgttacat aatcatcccc tccaaaggtc aaccctttga ttttgtcggt
     8281 ttctgattgc tttgcactta aaaatataat tgggcataaa acatcatctc taattgcctg
     8341 acatacctca aatccattca ttttaggcat cataatatcc agtataataa ggtcaggctc
     8401 ctgttttgat aactctatac ctgcctgacc atcataagct atcaacactt catgtccttt
     8461 tgccttcaat tcatcctcaa gtagcattac taggtccatt tcatcatcaa taatcaatat
     8521 tttactcaat ataacaccac ctttctctta tgggaaggtc agtattaaaa caatattcgt
     8581 ctactaataa cttttccttc cctcccacat tttaaaccaa ataacgcctc ctatcaagaa
     8641 tataatcaaa cctattgcta ctactataag ctctatacta gctgtttgca tcactttaga
     8701 gtaaaatgcc tcagttgaaa tagttccagc ttctttaata aaataaattc ctggaagcat
     8761 acctagtttt actggccatg tccatggaac aaatgcccat actttttcac caacgctagt
     8821 taatccaagt attgctgcca tcaaaattcc accgatacta ataccaatag atgcacccat
     8881 accccatgca aaactcaccc aaagatgaat agctaatagt ggaagtgtcc caatcaccac
     8941 aagtcctgct gctgacagga ataaccatat tgttgctcca gatggcactg caatactcat
     9001 tcctatgcaa agtatcaacg tagcaataaa tgtgcatata gttaggcaaa acagaagaaa
     9061 aagaaatttt cccagatata atctaacacg agaaatacct acacataaaa aaccattaaa
     9121 atttccagca agctcttcct cctgcactat aaatcctgct aaaataccta ctccaatagg
     9181 aatgatgaat acagtccata ctgtaaaaaa tccttcaaaa gcaaattctt gtgtacttcc
     9241 agatttagtt gtgagataaa aaaccacaca taatgaaaaa ataataggca tacaaagagt
     9301 aagccactgg acagctgtcc tctttgctcg aagccattct gatgataata gctttatcat
     9361 ttgtcatccc tcctattgaa ccaagctgca gtaagcgctg ttaatactat aaacacaacc
     9421 aaagaaacaa caatcccaat aggaatcact gaagtattca aaagtgggtc tccagcttct
     9481 aatatagtac cgttaggatg tatgttgatg attgggcata ccattctaac tgcccaactc
     9541 caaggtactg caatccaaaa tggttttgtc gcagcaagga caccaaatat catacctaaa
     9601 gctccaactc ccatacttag gaacatacct ttccatgtag ctagccatag ctgaagtgga
     9661 agtatagcaa gagaaacaac ccagcataca atacttgccc ctataatctg tccaaaagga
     9721 actggaccag ctttcaatat taaaccagtt ataattgtta cgactattaa aactaacgtt
     9781 gaaataaaac tgtagactgc catagctata actttattaa tccaaagtgt cataggagac
     9841 acattgtgtg tacgtaaagc acgataattt ccagcttttt tttcctggaa agctaccaaa
     9901 gtagcaaaca atgcaaatcc aataggcaga aataaaaatg accaccagtt aaaaattaat
     9961 gctaaaagac tttgccacgc ccaagaagtt gtttgagata atggattttg catgagtgtt
    10021 gattgcatta taaatgcata tgctgcaaaa gacactggga aaaaaacaat aagtttaccc
    10081 ataaatgttc ttttatactt taaaagttct gattgtaaag cattcatctg ttttgtcctc
    10141 ctttaagaat aacatctgta aaaaattctt ctagattttc attagggtca ggcattcctt
    10201 gaaatagtag tcgtcctcca tttataattc ctatattatc tacaacttga gcaacctctg
    10261 atagaatatg gctagaaaga attacagtta tacctttttt tggaaaagaa gcaatcaatt
    10321 cacgcaattc ttgaatgcca aaggggtcaa gaccattggt tggttcatct agtattaaca
    10381 actctgggtc actcaaaagt gcaatagcta ttcctaacct ttgtttcata cccatagaaa
    10441 attgtgaagc tctcttttta cctgtatttt tcaaatttac tatttccaac acttcataaa
    10501 ttttatcttt aggtattccc aatatttttg tatgtactaa caaattttcc tctgctgtaa
    10561 ggtttccata caatgcaggg gattctatta aagaacctat ttttgcaaga gattctcgtt
    10621 tccaaggctt tctatcaaaa ataatacgac catctgttgg gcgaagaaga ccaacaagca
    10681 ttttaagtgt tgtggacttt cctgctccat ttggtcctaa aagtccatat atactatttc
    10741 gttcaatttg tagagaaaca ttgttaactg ccatttgttc tccatatctt tttgataagt
    10801 tttttgtttc aagaattaaa tctttcatat taatttctcc cttcttgtaa taccttaaat
    10861 ctattactaa gagaatacta acatgaaaaa ataacaattc tataacacct actaatttta
    10921 ataaaaatta aaataaaaaa tagagttacc aattttattt tggcaactct atttaagttt
    10981 atgttttaca gttatattag atttaagctt agtttcatta tcacactaac ttcaaattta
    11041 ctctataatc cttaaatcaa gcactaattt attaatcaaa cactaatcta ttaatttata
    11101 tttctttaga catctaatat agtttgctac taaataataa ttacttatat catatttatc
    11161 taatccttta aaagaagtta tttcatcatt aagctcatca ctaccttcta caaaaacaca
    11221 acctgtttca aattccatac ctacaataat cttacagcca ctataagttt cctcttgagt
    11281 atcgtacaat ttttttgcat ttccttcatt tggaattgta aatacaatag gttttaattc
    11341 tttatcactg gaaatctttt gtgaaaaagg cttgtttaac ttaatattat tgataactac
    11401 atgtttaaat tcctctgaag tttcttctac tattacatat tttcgcttta aaaactctat
    11461 tatttctaaa acagacttag aatttacttt taaaatactt ttatattttt catataaaga
    11521 tttccactgt tcaatttttt cctgtggtac ttcttggata tgtgtactta ttttttcttg
    11581 agatattttt ttcaattaaa ctctcctttc aatacttaaa tatccttata aatctcacat
    11641 gactaaagtt acaaggttcc tacttaatag aattttgcat acttaaatat gtcttattaa
    11701 ttctctacag gatatctccg tagtccctac gattcttaca taagttacct attttaaatt
    11761 gttattagtt agattataaa aattatttat tttatcacta atataatact cttaaaaact
    11821 ttacaaatca atttaaattt gctacctttt atttataaca acttatagta catttctcct
    11881 atgcttttca atatattact tttaaaagga ggttttcctt atgattttaa atagaactga
    11941 gcttttccga ggatattctt taaatagatt taacgagata tgcaatatat tatcagtaaa
    12001 taaaattaaa tataagtata aagtgaaaaa taatgcttac tctaaaaaac ctttttctaa
    12061 tgaaataaca cttggcaatt taggtcaaaa ggaagatttc tcttatgagt acttaattta
    12121 tgttcataaa gacgattatg aacaggcaga gtctctaata aattctaagt tgcgatagat
    12181 tatttataaa acatgacatt ctgttgtcat gttttatatg ttattatact ttcaaatatg
    12241 ctactatatt ttcaaaattg tgtgctttaa aactagcttt ttatagtaat ttaaagctta
    12301 aaactaagga gactcgttat atgaaaataa atataaaatc aaagttaagt gaatttatta
    12361 aacagaacaa tctttttgat gacaacagaa taatatccta ccttcctaat ataacaatag
    12421 aaacagacaa aataaaaact ataatgatta atgaggttgt cccaactaac accaatgacg
    12481 atttttatag tgtagataaa gatgctgatt atttaaaaac tactattcct ttatttgaca
    12541 gtgcaggaat agaagtatct aacataaatg atatcctaga tatgggtatc tatataacta
    12601 atgcagtaaa acttcctaaa tcagaatata caataactcg tgatacaatt aaattacata
    12661 tgcctatttt agaagaagaa attaaattat ttaaaaacct agaggtagta atgttaatgg
    12721 gagatgttgc aaaaaaatct tttaatatga ttactaaaaa gcatataaaa aagaatgtca
    12781 tcccttccat ttcaacatat aaacttcgta ataatgaact ttattatgat aatttaagag
    12841 tatttccatc atatattatg actggtggaa acatactgat tgaaaaatca aaatttgaaa
    12901 tggcatcaga agatataaaa aaaatgttcg agattataaa cggttgatat tacaaaataa
    12961 taaaaactaa tttagccaaa taataagtgc ctcaaaaact tctatttatg aggcactttt
    13021 attttatatt agtgtaacta tatactatta acataattta ttatttatat tgttcttttt
    13081 aatctctatt ccatttagtc taggtaacat tactctttac aagattactt tatatttctt
    13141 ttatatctct aacgttttta tttaataaaa caattattaa tacaagtcaa atatatctat
    13201 ttatctaaaa atatatcttt tacagtaata tctggatggt gatacttctt cctattcgtt
    13261 aaaaaagatt tttttacatt aatagcctct tttggacacc agtgaaaaca agctaggcat
    13321 ctttcacact cattacccca ctttggcttt ttatcaacta tacttatatt ctctacagga
    13381 caaacccttt tacatgtccc acatccatta catttatttt ctgcaaaaaa tttagtagtt
    13441 gcaatttgca tattcttacc tactaaacga agtacaacac tatctatatg tgactttaaa
    13501 ttgttttttc cttctatatt tccaatctcc aagttattga tacattttgc aatctcagct
    13561 accctattag tctgattttt caatctttct tcttcaacat ctgaaggtgt tatcaaagca
    13621 tttccaggca tattgattaa aaaaccagca gataattttt tccccctttt tttaagtaac
    13681 ttatctatag taaataaact atgtccagct accccattac aagtggcaat ggcatatata
    13741 tgagtattaa tattaaaatt taatttctct acaaaagact ttattatatc aggaacatca
    13801 gcaaaataaa caggaaaaac aattaccaat atatcttcat ttatgtctat attattttct
    13861 tttctatgta tagctaaagg tataacttct ttacttccat ttaacgtttc ttttaaaatc
    13921 tttgaaacta ctagcgaatt tcctgttcca gaaaaataat aaatcttact attcattctt
    13981 atccctcttt ctatatttct acatcatcaa tagttaaaaa gtctcttaaa ataggattta
    14041 tatttacttt ccaattctat ccaaaatatc agtaaacaaa ggcaattctt catcagagat
    14101 agatttaaat gtctctgttg catatatact tgcaatttca tttaatggtt cttctaattc
    14161 tctacctaaa tctgttagga atatttgaaa cttacgtcta tctttaacat ctactttacg
    14221 gacaattaat cccttatttt tcataacgtc taaaattctt gttgtattag atttatcctt
    14281 acatgatttt tcacataaat ctatctgaga aattccatct tgctcccata gaaaccttaa
    14341 taacgcataa tggtcagttg ttatatcata tccattttgt ttaaacatct tttttaaatc
    14401 atcttttact tttaacgaag tacttactaa cttagttgta gcttcatata gtttctcaaa
    14461 atctttcatt ttacctccat ttaaataatc ccctgctcct cccatctatc aattattccc
    14521 ttatattgct caatatcttt aaaagccatt ggctttaaaa cagcatttat agcaataaaa
    14581 ttatagattt gagaaagcac aggtggattt ttcttttcct ctatgcaata aataaatttt
    14641 ttagatacct tttgtatctt tgattctact ttttcagtta taggattttg attaaatcca
    14701 gtttttatag ataaactacc acaaacataa cctcccgaat ctaagaccat ggattttata
    14761 aaagaaattg cttttgaacc acgtgcattt tcatatgtag taactgctat acatggctta
    14821 cgatacaata atctttcaag catcatatgt gctctatctg taaacacctt gaaaagtcca
    14881 gttacactac ttccataagt tgggctacca aatataacac catcactctt agtaattata
    14941 tcatgaatat actctacttt atcattttga ttgatacact tgcccatttt ataacaattt
    15001 aaacaaccaa tacaatatga tggatttacc tcacttaaat caaaataatt aatttttaca
    15061 tctggtatta atctttctat atctttatac aagtaagtta aaacttttga tgtagctcca
    15121 ttttttctag ggcttccatt tataactgta attatcattt ctatatctcc tttttagttg
    15181 ttatatcaac aataagtata tcaactatat ttttagctgt caatataaac catttattta
    15241 aagctatact tacacagctt aaataaaagt tttatttaac tacatgtgct tatttaatca
    15301 actataaatg aattattttt aataattgac aaactaaatt aatgataata tactttatgt
    15361 attacaaatg taagtagaag gaggtatagt atgaaaaagt taccacatat ttcagaagca
    15421 gaatatcaag ttatgaaaat tatttggaaa tatgctccta tcagcactac tgaagtaatt
    15481 gaaaagttgg tagaaacaag tacatggagt cctaaaacaa ttcaaacaat gttattgaga
    15541 ttagttaaaa aaggagcttt aacatatgaa aaaaatagca gagtattcgt ttatactcca
    15601 cttgttaaag aagaagaata cgtagctaca gaaagtagtt cttttttaaa tcgtttttat
    15661 aatggtactc ttaattctat ggtactaaac tttttagaaa atgataaact atctgaagat
    15721 gatattgagg aactgagaga aatcttaaat aaaaggacga ctaaggggga taaataaata
    15781 tgtatgcccc attctttata cgatttttac tcagtacatt aaccttatca atattaattg
    15841 ttataatttt acttgccaaa aaagtattta aaaaacatat ttctgcaaag taccaataca
    15901 atattggatt tatattgctt ataatgttaa taattccatt tgtccctctt aaatatatta
    15961 atcttggaaa tgtatttaac tatttatata cttttaatca acttggaagc agtactaata
    16021 ggacttctat tataaaaaat agtccaaatt ctataattaa taactcaaat gcattaaacg
    16081 atttttcagt atccctaaac caagttaatt ttgatagttt aaatatttta ttactaatat
    16141 catggataat tggaatttta atcatgatag ctattactat tcattgcaat attaaaataa
    16201 aaaatatgaa aaaatcaata caaattttaa gagatgagga gatagtattt atatttgaaa
    16261 agtgtaaaaa agatattggt ataaataagg attttatact atgcaaatca cctattataa
    16321 atacacctat tacatttgga tactttaaac cacacattat attaccagat aagtctatat
    16381 ctaaattatc tttaaaagat ataaaatata tcttacttca tgaattacaa cactttaaaa
    16441 ataaagatat actgataaat tatataatgt gcttccttca aatattatat tggtttaatc
    16501 ctcttgtatg gtatgcattt aaagaaatgc gtatagaccg tgaaattgca tgtgatattt
    16561 cagttttaaa aaagcttgat aaagatagtc atatagaata tggacaaact ctaataaact
    16621 ttgtggataa tatttcaaga ccatcaaatc ttacacttac atcagatatt ggtgggtcaa
    16681 aaaaacaaat aaaaaagcga ctattgaaga taatggattt tcataatgaa tcaaatacgt
    16741 taaaattcaa aagcatttta atttttacat tgatagcttt aattgtattt ggaaatattc
    16801 ccaaactatc tattatggca gatatcaacg aaaaatatag acctacaaat gaacaaactg
    16861 tgtacgagga cttaagtcag tattttaaag gttttgatgg aagttttgtt atgtatgatt
    16921 taaaatccaa gcaatatcat atttacaata aagaaaaaag taaaacaaga atttctccaa
    16981 attctactta taagatttat agtgccttat tgggattgga aaatgggata ataacaagaa
    17041 ataattcaca tatcccttgg gatggaaagc atcattctaa tagttcttgg aatgaagacc
    17101 aagacttatt ttctgctatg gaaaactccg taaactggta ctttcagact ctagacaaaa
    17161 aaacagggct aaaaaacata caaacttatc ttaagcaaat tggatatggc aattatgatg
    17221 tttcaggtgg tatctcaaac ttttggctag aatcttcatt aaaaatatca cctattgaac
    17281 aggtagaatt acttgaaagt ttttatacaa ataagtttaa atttgaagat aaaaatatag
    17341 aaacagtaaa agattcattg ttaatatcaa aaaaggatgg ggtatcactt tttggtaaaa
    17401 caggcactgc taatataaat ggaaaagatg taaatggttg gtttataggt tatgtggaaa
    17461 aaaatgaaaa tacttatttc tttgctacaa atattcaaag caaagattat tctaatggta
    17521 gttcagcatc taagataaca ttatccatat taaatgacaa aaaaatctat aatccttaat
    17581 atatttactg tacattctaa aaaattcaca taattttcat attaattatc gattaaattc
    17641 atatacaagg cttattatat aaacatagaa gatagtaaaa ctagcttaaa aacataatat
    17701 aataccctat acaaatagtg gcaactctcc ctcacatcct caattgttgc cactatttaa
    17761 ttttttgtat gaagttaaat atattttcat catttcaaca atttgggtgc agtacaatct
    17821 ctattttgag acaacctctt taattgtttt agagtttatg aattatttct catactttaa
    17881 aaagattgaa ttactcttga taatcttctt ttgtagtcat tacatatcac ctactactca
    17941 acacatattc tttgacctat ttttacatcc ttgtttatat ccaaaatatt taccttctta
    18001 ccatctggaa acaataccat tgtcaaagta gactttccta attcatgtat tctctttaaa
    18061 tcaacattaa caagaacatc tccctgttta accatatctc cttgtttaac agcaatatca
    18121 aagccatctc catttaatgc aactgtatca attccaacat gaattaatac ttctccatta
    18181 gttcctttaa tcccaaatgc atgtccactt gaaaatactg tttctaattt acctgaaata
    18241 ggagaaacaa tttttccatc tataatatca atagcaaaac catcacctaa cattttttta
    18301 gaaaatgttt catctggtac attttctaat gcaacaattt tccccttagc tggagacaca
    18361 aagttttcat tattttcatt aaacactaat tcttcgtctt catcttcatt tatagcttct
    18421 ttaggcactc caaataaaca tgttacaata aagcagaatg caaccacaaa tattgatact
    18481 aataagtatg tgattaaatt atatccatca tagatataca ttaaataaga aggtaaaact
    18541 gctaatccat atgaattaga ttgaatatgt aaaattgata atattgctgc tcctaaacct
    18601 gaagatgcac acataataat caaaggcttt aaattatatc gaagctgaat cccaaataaa
    18661 acaggctcac ttataccaaa aagttgagat agcattgctc caactgcagt ttgtttaact
    18721 tgattatttt tagatttttt cataaatgct agacatgttc caatattcgc aaatccatac
    18781 attgcacaaa gagtaataag tgcattaaat cctgtatttg ctaatagtga tgtttcaatc
    18841 attacataag tatgatgtaa gccagtaata acagctaatg gatatgtaaa tccaataata
    18901 aatcctccaa ttcctaaagg taaaccaatc aataaactga tgatatcaac catttttaat
    18961 tctattgtat gcattatagg ccctacacct aacaaaataa ctaacattgt tattagcata
    19021 acaaagaatg gtgtgaaaat aaggtctaat acattaggca tatgtttacg gaaccatttt
    19081 tcaagattag ctccaataaa tgctgtaaca atagctgtta aaacacttcc ttgacagcct
    19141 acaataggaa taaatccaaa tgccataatt gcttcaactt catttgatgg gtcagcaaca
    19201 gaatatgcat taggaagtat tggagaaact aacataagtc caataacaag tccaataaca
    19261 ggtgttcctc caaatacctt aaatgctgac catacaataa ttgctggtaa aaaagcaaat
    19321 gctgtttctg taagaaccga aacaagtgtc tgaatataca atggaatatt tgcagaagac
    19381 attccaaata atccaagtac attatcatta aacaaacaac cctttaatcc caagaataat
    19441 cctgttgccg caataactgg aataatagga acaaatatat ctgctagagt tctcataact
    19501 ttcttaaatc cagtttcatt atttttaact aattcatctt gttctttttt tgataaagtt
    19561 ttcaatccca ttttttcaac ttctgcataa actttattga ctattcccgt tcctagaata
    19621 atttgatact gtccagaagt aaagaaaaca cctttaacct cattaacctt ttccactttc
    19681 ttatcattaa taatttctct atctttaacc atcaccctta atcttgtggc acaatgtgta
    19741 atagaaataa tattattttc accaataatt tcaataagct catttgctat tttattatat
    19801 ttattcattt ttctacctca ctttcaataa tactgcttga aatggtttca aactcactgt
    19861 tttatttagt agatttatat catctaaatt ttggaataca atatcatatt tttcttctgt
    19921 ttttatttct acaagtttat cagaaaaatt ataataacaa tcaataacta ccaaatctaa
    19981 ttgccttcta tacgcaataa cattgtcact ttctatatca ataggttcta ttgaaccata
    20041 aatcaaacaa tcagaatact tactatcttg tctcaacttt atcatctttt tataaaattc
    20101 taaaatactt tctgaattgt taatctgact ctgtgcatta atatctttat aattttcaat
    20161 aacctttaac catggtgttg catctgaaaa acctgcattt tctttatctg cccatggaaa
    20221 tggcgttctt gcattatctc ggcttcttaa attcacaaat tctaacgctt cttcttctga
    20281 gtaaccttca tctattgaac gatagtattg gtcaatgcta gataaatcat taaaatcatt
    20341 tatggtatct cttttaaaat ttgtcattcc taattcctgt ccctgataaa taaatggtgt
    20401 tcctcttaaa aagaaataca ttgctcccaa catttttact gcatcatcat tcctttcgta
    20461 ttgcaaaaga tactttgttg tagctcttgg ttggtcatga ttttcaataa aattagctgc
    20521 ccatccatgt tcctgaacat acatttggct atctaaaatg cattttctta aatctggaat
    20581 tgtccatgaa attcttttaa accactcact accagaagca acatctaagt cagcatgttt
    20641 aaagtcaaag accattgaaa agaaaccatc ttctccaata aaatcaccta gttggtcata
    20701 gctaatacct gctgcttcag caacagtcat acaatcatat tttttaaacg tattttcttt
    20761 taattcataa agcatttttt ctattccagg ctgatttcta actgtttttg tacatttaac
    20821 aagaccatca acaccatcac tttctaaact ttgaaaactt aaattctttt tgataaatgt
    20881 aatagcatca actctaaatc ctgccactcc catttgagac caggtattaa tcatttggta
    20941 aagttcactt cttaatattg gattttccca attaaggtca ggttgttttt taccaaaaga
    21001 atgaaaataa tattcgtctc tattctctac cttttcccaa acacttccac caaaaacaga
    21061 cctccagtta tttggtaaat cgtttccttt tttaaaaata taataatcat gataaggact
    21121 ttctggattt tttaatgctt cttgaaacca aggatgttca tcacttgtat gattaagaac
    21181 taaatctaat ataattttga ttcctctatc tttactttct ttaattaact cttttaaatc
    21241 ttccatactt ccaaattcac ttgcaatatc taaataatca ctaatatcat acccattatc
    21301 gtccataggt gatttaaaaa ttggacataa ccacaatgat gtaataccta aatcttttaa
    21361 ataatctaaa cgagaaataa ttccctgtaa atctccaatt ccatcattat tactatcttg
    21421 aaagcttctt ggataaattt gataaacgat ttctttttgc caccatttct tttccataaa
    21481 ttactccttt ttatatgata tttcttcttt ttaattcttt ataaattcca ttatcgaata
    21541 tgtcctcaca tatagatgtc gctatatctt taagttgttg atgactattt ttcattgcaa
    21601 ttgtaacgtc tgatgcttga aacatgacaa tatcattttg accatcacca aaacatatgg
    21661 tttctttttg agttacccct aatctttctt gtaatctctt aattgctttg cccttatgaa
    21721 aatctttttg aataatttca taatactgac tagaaatatc tctctgtgct aattccattt
    21781 tatcttgtag tatatctttc acttcgtcaa ataccttttc atttgaccat aaacaaattt
    21841 tatgtacatc ttgtgactta tactcttcaa tattattttc atatgttatt ttttcttgga
    21901 tatgttgttt atttatacaa gaattagttc ctttcaactg attcattgtt tcaaatattt
    21961 ctttggcttt ttgattcatg aaaacttttt cctgactctc aatcgaaaaa gcaacttctc
    22021 tcttttttaa caaacaaaca acttccttta tcaatctttg attaaaagat tgattataaa
    22081 gtaattcacc atgatattga atgtaattac ctcctcctgc aatatatcca tcaaccccta
    22141 atgataaaac atcatcttgt attgtaccta tacttcttcc agtacaaata acaacactgc
    22201 aatgattctt ttgacataaa cgtatagcgt gctttgcaga ttctgggatt ccatatacct
    22261 catccctaag cgtaccatct atatcaagta ttaataattt gtacaatttc atcttccttt
    22321 cttactatca aaatcataat agcattcaaa aaaaaagata tcaatatttt gatatctttt
    22381 aaaaaatatg ttcattttta gcacaaattt tcactttcgg ggaatatgtt cactttctaa
    22441 atacatcttg tatttaatat aaagcagttc aattaaaata aaataaggtg ttgttacatc
    22501 gtagtccagt tgtttatcta cagaaataga tatagattga atatacaggt tttcatcaca
    22561 taatcttgct aaaggattac ttatcatttt tgtaatagaa accatataag ttcctttcat
    22621 tctcatttct ttagcaaaat caataattgt agatgtatct ccattcaatg agataaagaa
    22681 aatcatatca tctgttttta taaaatgatt aagcgcctga atcatatcat aaccataaac
    22741 atcatatata attttccctg ttggtaagaa aatccttttc atctctttaa caactctttc
    22801 ctgagaataa ccttctccaa agacgattat acgatttgcc tttttcattc tttcaaacat
    22861 aatcgaacaa tccttcttct ctatatcatc tatcacctta ttataacaat ccattaattg
    22921 gtgtttactt tgatttttat attctacttc tgtttcattt aagcgtataa taactcttaa
    22981 ttcactatat ccaggtaatt gtagtttttg agcaaaacga gaaagtgcac ttgtagatat
    23041 atggtatttg tctgcaaatt catctataga aagtttaata caatcttctt tatgattaag
    23101 aatacactca cagatatact tatcatttgc tgtaaatgaa ttataatatt tattgaaaag
    23161 tccttcaatt ttcattcttt tctccttcta tactctatgt aattcatatg aagcaaatct
    23221 aatagagcat aaaatgctcc tacgacttca taagatatag cttgtaatgt atcaagtttt
    23281 tgagtttcaa catacagatt atactcacaa agtgttgcaa ttgtattatt atcaagccta
    23341 gttaaagaaa tcgtatgtat atgattgttg ataaattttg caatttcaat tcctgatttt
    23401 gtttctccag acaaagaaat gataataaat aaatcacttt tttgaaatga ttctctcata
    23461 aattcaattt cacctatatc aaataagtca ataacacatt tacctactga aagaaatatt
    23521 ctctttattt cttgagctaa acttttctgt tcattacctg tcccataaat ataaattgtt
    23581 tctgcatcat atattatttt acaaatatct tgaaatgata ttttttgtaa ttcattcatt
    23641 aatagtcgat aattctcgca aacatcatga aaattattat cattattaac aaattctagt
    23701 tgattatttt taagtaaata ctttaaatca gagaatgaat ctaatccaat ttttctacaa
    23761 agccttaata aagtagctct agaaacatta cattcatttg caattttttc acacgaaaaa
    23821 ttacggtatt ctctttgaca aatcaatatt tgtttaataa taattttatc attttcattt
    23881 aaactattat aactttggtt aactaacgat tctaatctca ttttttccac tccctaaata
    23941 ttattttaat atacattagt tgctttttct agtgaagagt cttaaataat aaggataaaa
    24001 taatcatttg attttatttt tattaaaata aaaataacct gctaaaagca ggcaccaaaa
    24061 agtacttatc ctttcgaaca tgtactttag atattgcttt cttcggaagc ttcatctctt
    24121 catactctaa aatagagttt tttctaactc aaaagtattt tccatggtag aaattacttt
    24181 aattcatctg ccatttattt tttatcttat ttaataatta ctattacttg aatatttact
    24241 tctttaagta aatttcatag ttaacaaagc taaaactatt attatagatg ttaaaataca
    24301 aaattataag tattgttata aatatgctta cagctacaaa tatacatgat atattaaaaa
    24361 gaagctgtaa attattatca tacatacaag caactatgcc tctattaatc catgttaatt
    24421 tttattattt aatacaagga gttccatgag acatacatat ccattcatat tttaaattat
    24481 ccaattctct tatagatttc atcatctttt tataatccca attccatgga ttaggataag
    24541 gaactatatt tccattctta tttttgacta aatcacctgc aaataataca tcttcaaaaa
    24601 tcatgcagac atgccctgga gtatgaccag gagtatggat aatttttata ttcccaatct
    24661 tcatattctc tccataaggt tcaacatcct taatgagcct tctggaaatt gcctttccaa
    24721 taaacttctt aaacccaggt ctatccttct ctcctataat aaaaggtata tcctctacat
    24781 gagcccatac ctttgctcct gttttctctt tcaataattt aatattagat atatgatcaa
    24841 catcatgatg agttaataaa atatgcttaa tatctgtaag tttaatgcca agtgttttta
    24901 attctttaat tatatttttc cccataaaag gaagacctgt gtctataaga ataatttctt
    24961 tatcgaacac aataaaacaa tttgaaccac gagtactatc taacattaat acattttctt
    25021 ttatcttcat aaataaattc cccttatttg gtattttacc tatataatta attaataaag
    25081 ttataaatag ttataatact tgatattaat tttatttttt attaaagtat actataggta
    25141 tacaaatatt atatttacat aagtatacca tcagtatact tttttatcaa tagaaaggaa
    25201 taatttatat gtcagaatta tcaaaaagag ataaagaaaa aatacaaaga gaaaatgaga
    25261 ttattgataa ggcagaaaaa ttattttgtc ttaatggatt tgattaaaaa atagctacgc
    25321 acactcgtga ctttagtcat gagttagtag ctaaaatagt cagcatatag ggaaacctat
    25381 atgtagagat aggataa
//
LOCUS       DAGQWR010000054        25124 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.054, whole genome shotgun sequence.
ACCESSION   DAGQWR010000054 DAGQWR010000000
VERSION     DAGQWR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.054"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(117..1331)
                     /gene="dpaL"
                     /locus_tag="MIW95_001870"
     CDS             complement(117..1331)
                     /gene="dpaL"
                     /locus_tag="MIW95_001870"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBY3151198.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLRDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(1954..2493)
                     /locus_tag="MIW95_001871"
     CDS             complement(1954..2493)
                     /locus_tag="MIW95_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HBY3151199.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            complement(2570..4375)
                     /locus_tag="MIW95_001872"
     CDS             complement(2570..4375)
                     /locus_tag="MIW95_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3151200.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIYKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNVCKNKDINIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(4783..5160)
                     /locus_tag="MIW95_001873"
     CDS             complement(4783..5160)
                     /locus_tag="MIW95_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBY3151201.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6011..6496
                     /locus_tag="MIW95_001874"
     CDS             6011..6496
                     /locus_tag="MIW95_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151202.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFDLDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(6696..7235)
                     /locus_tag="MIW95_001875"
     CDS             complement(6696..7235)
                     /locus_tag="MIW95_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY3151203.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEASCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVAAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(7228..7641)
                     /locus_tag="MIW95_001876"
     CDS             complement(7228..7641)
                     /locus_tag="MIW95_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY3151204.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(7677..8798)
                     /locus_tag="MIW95_001877"
     CDS             complement(7677..8798)
                     /locus_tag="MIW95_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBY3151205.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            9397..11049
                     /locus_tag="MIW95_001878"
     CDS             9397..11049
                     /locus_tag="MIW95_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBY3151206.1"
                     /translation="MNNKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYKMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSDILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKIEYEITINAITGKILEFEIDD"
     gene            11346..12095
                     /locus_tag="MIW95_001879"
     CDS             11346..12095
                     /locus_tag="MIW95_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151207.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFDIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYAASFANRILIFKDG
                     HIIKELNKQPNNRHTFYELIIQEIAKLDTKR"
     gene            12108..14135
                     /locus_tag="MIW95_001880"
     CDS             12108..14135
                     /locus_tag="MIW95_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY3151208.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFITGLVLGALSHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAISNGKFLYKTSLVKLIRHEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLINGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            14279..15862
                     /locus_tag="MIW95_001881"
     CDS             14279..15862
                     /locus_tag="MIW95_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY3151209.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFL
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRNQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDIIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            15834..16454
                     /locus_tag="MIW95_001882"
     CDS             15834..16454
                     /locus_tag="MIW95_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3151210.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYEEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTNMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(16642..17568)
                     /locus_tag="MIW95_001883"
     CDS             complement(16642..17568)
                     /locus_tag="MIW95_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBY3151211.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSENS
                     NAEIVTKNSDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNTYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENLDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            18178..19098
                     /locus_tag="MIW95_001884"
     CDS             18178..19098
                     /locus_tag="MIW95_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY3151212.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHFVDIYLPIEPF"
     gene            19184..21604
                     /locus_tag="MIW95_001885"
     CDS             19184..21604
                     /locus_tag="MIW95_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBY3151213.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLALLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKILINQGNLNTFGTNLVNDILSKTRHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSDSEIGDEDDYAVIETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            21604..22626
                     /locus_tag="MIW95_001886"
     CDS             21604..22626
                     /locus_tag="MIW95_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBY3151214.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWICKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            22741..23487
                     /locus_tag="MIW95_001887"
     CDS             22741..23487
                     /locus_tag="MIW95_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY3151215.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            23573..24823
                     /gene="cme"
                     /locus_tag="MIW95_001888"
     CDS             23573..24823
                     /gene="cme"
                     /locus_tag="MIW95_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBY3151216.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHNIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLVMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIVAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
BASE COUNT         8833 a         3788 c         3331 g         9172 t
ORIGIN      
        1 atttagattt ttcatttgtt ttagttttta ttttatttaa agttttcact tatttaagtt
       61 tttattttcc ttaaattttt catttatttg ttaagttttt attttcctta agtcttttat
      121 ttactttggc attcaccatt ccaaactata tctctatatt tatcagggtc agtatctcct
      181 tctgtactta ttaataatac tttagagttc tcatctagtt tcaaagcttc tcttaagtct
      241 tttaaatctt cactttcaag gattgaaact aatgctccag ttgtaactgc tccagattct
      301 ccagagatta ctttctcatc tcctcttaga gggtttccta atattctcat acctttagca
      361 gatacccagt ctggtgctga aacgaatgct gatgaataat tttttaacac ttcaaaacct
      421 atagtgtttg gttctccaca agcaagccct gccattatag ttggcatatc tcctccaaca
      481 aatcttggtt ttccatctcc agcttctgct gatttatagt aacaatcagc ctcatcagcc
      541 tctactacta ctgttattgg gcattcatca tatattgaag caacataccc ttgaactgca
      601 ccagcaagtg aaccaactcc tgcttgaaca aatacgtgtg ttggtctatc tactccatat
      661 tcttttaatt gttctatagc ttctttagcc atagttccat atccttgcat tatccatgct
      721 ggtatttctt catatccgtc ccatgctgta tcttgaacca ttactccatg gtcatcagct
      781 tctgcataat cattagctaa tcttactgca tcatcatagt ttaagtctgt tatactagca
      841 tctgcacctt ctcctttgat tttatttaat ctaaattcag aagaaccttt tggcattaat
      901 acaacagatt tttgacctaa tttattagct gtccaagcaa ctcctctacc atgatttcca
      961 tctgtggctg taaagaaagt tatttctcct aatttttctt ttatttcttt tgaagttaat
     1021 ttgtcatacc ctaactcaga tatatctgta tctaatcttt gagctaagta tctacccata
     1081 gagtaagaac cacctaaaac tttaaatgcg tttaaaccaa atctataaga ttcgtcttta
     1141 acatatattc cgcttacgcc tagtgatttt gctaagttat ctaatttaac taaaggagtt
     1201 ttttcatatt gtggaaagct ctcatgaaaa gctttagcct ttgcaatttc ttcatctctt
     1261 aaaaactcaa ttcctttttc tttttcctct gatttaggta agttatttaa tttccatttt
     1321 atttctctca tttattttat attccccttt caaatttttc ggtgtcattt taatttttta
     1381 tctttgtgtc ttatagtgaa gcaattacta tgccaatatt atttattcta aaaacaattt
     1441 ttttatgttg gaattacata tatttataaa atttctttga attttataaa tattaaatgt
     1501 gaaaaataag atttataaaa gaaaaaattc tcatattggg aaatttttat caatacgaga
     1561 attatcaatt cgagaaatcg tttatttatt ttaattcttc ctttattatg ctcttatttt
     1621 ttatatgtct attgttttgt cagatatatt actactttta ttttctataa atatatggct
     1681 ataaccttct tacgcataaa ctctataaat ttatatactt tatattcttt attacccaaa
     1741 tattctttat tacccaaagc ctccaaaatg gcttaaatct tttcatagct tcttacagaa
     1801 gtttacatgg gcgtccctcc aataagcaaa agtgcaatat gtgtaccatt gtgtcctact
     1861 tgcgactatt gagctgtcga aattcgcaac ttcgcattta tctgatgaaa tgcactatgc
     1921 aaaatattca taacatccac aacagagtta aagttaccta ttcatattca ctgtaaaaat
     1981 ttcttccaca ggaaatgtaa ttttaaccaa tcctattgag acaaactcac cacttatttt
     2041 tttgaactca cgcagtaata catcacttgc ccgaaaaaaa gcctcacttt catcatctcc
     2101 agtaagtgct attgtgcaat ggggtaccca acgattgggg tggtaccatt cccaaccatt
     2161 tgaatcaaaa tcttgtagta cctcatataa ttctctctga aactgataca tactgctatt
     2221 cattataggt gatacaaaca ccgttttagt atcattaaac atgcctactg aaccaatgta
     2281 agcaggtatt accttatggt tttgtgcaaa cccctttagc ttgtctatgc aacaagcctc
     2341 atccacatca ttgaaacaag ccaaagtaag atgaggtctt gttttccatt ctaagtactt
     2401 cgtactaatt ttttcatcag cgattttttt agataaatca aaaagttgct tttccgtttt
     2461 tttatcataa tatagctcta tcgcatattg cataataaca cctccaccat aaagtaaaaa
     2521 tacctatcta taaaaacaca caaataccta tcattttaat attatattac tactgttcct
     2581 cttccaattt tctatatagt gtagccaaac ctatacctaa atcctttgct atcttcttct
     2641 tcgtctttgt atctcttcca tacttattca acaacttgtt tatgtaagtc aactctaatt
     2701 ctttaattga aagtatcctt tcctgattct ctactatccc tccaactatg tcatcttcaa
     2761 atattatatt tatatcctta tttttacaaa catttccatt aatattataa taatttaaaa
     2821 tatccaatgg aagcatatct ttatatatta ttccatcatc tccaaccaaa ttcatcatat
     2881 attcaactgc attttccagt tcccttatat ttcccggcca attatagtta atcaacatat
     2941 tcattacatt ttcttctatt ttatttaact gaatacctaa ttctaaagaa tatttagaag
     3001 caaacttcat cattattgcc tcaatatcat cctttctttc cctaagaggt ggcaaattta
     3061 taggaattac atttaatcta tagtataaat catccctaaa cttaccttca tttactaatt
     3121 tcaataagtt tttatttgta gcagcaatca ctcttatatc taaatctata agtttatttg
     3181 acccaattct cgcaaatttt ctctcttgta aaactctaag taactttgct tgtaaaggca
     3241 ttgacaaatc tcctatttca tcaagaaata taacaccttc atttgcaagt tcaaatttac
     3301 ctactttacc tccactacta gctccactaa atgcaccttt tatatagcca aatagctcac
     3361 tttcaaggag attttctgga atagcagcac agtttatcgc aataaatggc ttgttccatc
     3421 tgctaccttc agcatgtata gccctagcaa ttagctcttt acccgtacca ctttccccag
     3481 ttataagtac agttgaattt gactttgcta atttcttagt tctctctttt acttttaaca
     3541 ttgcctctga atttccaatt atagactcta tatcactatt tccccagcca ctattgactt
     3601 ttccatgtcc tttatgattg atatatgtct tgtcaaatat tattattttg tcgtattgat
     3661 ttatatatgg atataccgga attattttgg catttatatt ataaattttg ttatcaatat
     3721 caatttctag taattcatgt ccatttgagg aagattcaac agatactata tttactatat
     3781 tttctattat atttttatat atatctaatt tttttagagc tatattgttt atataagata
     3841 ttttgctatt tatgtctagt attataacgc ctttttcaac attttcaatg atttctatca
     3901 tcatatccat ttttctactg ctattttttg acgcttcatg ttcttctatt ttcatagaaa
     3961 ttaaatcgca tatatgattg acaaatttta aataattatc tatttttgca cttatttcta
     4021 cttttttagt tttatctgta ctaatagcac caattactcc tatagttcga ttattatact
     4081 ttattggaac tgctattatt actttattta ggcatttcat gtaatgggaa cattctatac
     4141 aaagctgatt atcacatata tccaatacga ctaattcctg acctgtctgt ataacattgt
     4201 cgtaaataaa tccttctgta acaacgcttt catttatctt tgatttatac agacctgtac
     4261 cactaattct tattaatcta tcatcaacta tttcaatatc tacatctaac acactagcta
     4321 ctgtttcagt gtattttatc atttcttgtt gaaattcttt taatttactt aacatagttt
     4381 cccccattag aatttaattt accaaattat cagtttagct atattttaac atatagtcac
     4441 tctttaaaat atatataaaa cattacttat ttatatattt acattaaaac acacaatatt
     4501 aatttataaa aagtttgttt tatatgatga ccttaattac ttaattataa gatacagaca
     4561 ataaagtaaa tcacaataca ataataaaat aaaaaaagct ttcctcatat aacaaatata
     4621 tttttgacat aaaaatatac attatatgag aaaagctcaa tagttaaata tttagataga
     4681 tttttatata gactttttta atataaactt ttttaattgt agactttttt tattagtttt
     4741 taatttttat tagttttgtt attggtttta ttgttataat acttataata ctgccattgc
     4801 ttcaatttct aaaactgcat cttttggtaa ttgagcaact tgtacgcatg accttgctgg
     4861 tggattttct ttaaaatatg tagcatatac ttcattaata tcattaaaat cctttaaatc
     4921 ttttacgaat actgtagtct taaatatttt atccatactt gtaccagctt gttctaatat
     4981 tgcttttaca ttatctaaag aagcttttgt tgcttctttt gggtcagata ctaactcgcc
     5041 agtcttcata tctattggaa gttgacctga tgtagaaact atgtttccaa cttgaattgc
     5101 ttgagaatat ggtccaattg ctcctggagc tttctctgta gctattacta cttttgacat
     5161 gacttcacct cctattatta agttatattt acaggaaaat aaaatttcct aaacaccaat
     5221 attaatacga acctttagat taaatttcaa atctaaaact tcaaataatc aatacttaga
     5281 tgattatgtt tatttgtgtc ttgttaactt ttattaaagc aattaacgtg ccaaacttta
     5341 tttatatata aaattttgtg aagtcattca aaatagttac ttctgaattt tttcgtaaaa
     5401 aaatgcttta aaattacttt tttatgatgt caattttttt ctcatatcta gaaatttacc
     5461 aacatgagaa ttcttgtttt gagaatttac agattaaagt tatttttcaa tcttaaatta
     5521 tcaagttatt acatatgaaa gaactatagt tgacaatccc actattataa ataataaaac
     5581 ctttacccat accttattta ccctagttat catagagcct attgaaaata taacagctaa
     5641 atataataat aaccaaaaaa ttatcaatat ctaccactgt tgctgaaaat ccaaaaaact
     5701 accttttgat agtattactt aacaatctaa ttaatctcat aataaattta caacttataa
     5761 ataaattgtt atattggccg tatctcaaca actttctcct ttcctagttt taaatctttt
     5821 atagaattgt accacgtcta agtaaatttt catactatta agtcttattt ctaataaatc
     5881 attagctctc attctatgcc caataatgta agattgtagt aattttattg attttaatag
     5941 ttttatttaa tatacttata ttaattattt tcatgtgaaa ttattactta tataggggga
     6001 tttttttaat atgagcaaaa gtgaattaac ggcagaaaca acagaagaaa tgttagaagt
     6061 actaagtggt aaagattatg atattgcatg tcatttacat gaacttggta aatcattaga
     6121 ttgtaaaatt gaaccaaaaa caggtgctcg ttcttacaaa atagtatatt caactaagaa
     6181 accaaaacgt agcttattta ctattgaatg taatgaaaaa aaatggagag ttaaagcaaa
     6241 tctttttcat ctaaatacat ataaagatgc tgtggaggaa tgctctaaaa ctattaaaga
     6301 tagtataact aaaactcgta cttgtaagaa atgtaattca aagtgtattg gaggttcttc
     6361 gtttgattta gatggaaagt cttatctgac ttgcatagga agtggtcatt attttgcaaa
     6421 tatggaagaa atggattgga aaaacctaga aaaattaatt actaaagaaa ataatattat
     6481 gcaggaatct gtatagtttt ttaagggtaa ttattaaaat atatgagtaa caacagatac
     6541 ttaaataggc ataaaaaaat aaagggtata ataaggatat agaataaaaa taaatgatat
     6601 agcacaaaga ggctgtctca aaatatagat aagcctcttt ttattgttga aatgataaga
     6661 atatattcga ttttatacaa ttttatacaa acatactaag ccaacttacc catttcatat
     6721 gcctgtttca ttgccttact tcccttaata tcacctacat tccaagcacc tacaccatat
     6781 actatacctt tttctttaga accatttaga catgatgtaa atcctctaaa tccttctatt
     6841 gttctttcca tatcagccac attttcatct gctgctgcaa cgataaaata gaaatcttta
     6901 tcacaaattt cagtatatct agagcaagtt ctatcaatca tagttttcat ttgcgcattc
     6961 atagtataaa aataaacggg agtcgctaaa acaatcacat ctgccccaat cattttttca
     7021 agaatttctc ccatgtcatc tgtctgtgaa caacttgctt ctttttcata acaagcacca
     7081 catccagtac aatagttaat atctttatct tttaaaaata tcttctccac ttgattttta
     7141 gattctaata ccccttgtat aaactggtca cacaataagt cagaatttcc accttttcta
     7201 ggactagctg atactattaa tacattttta ctcataagat actgcctcca ttttcttatt
     7261 tctactgctc tttaatactt tttctttctc aataatacct tcttcatatc tatcaatctt
     7321 aaaatttaat cgttccaaag ttttttgcat ttcttctatt ctttttgcta gttggtttct
     7381 ctgctctatt aaaagttctt ttctagcttt gatagtactg ctcccttgtt gaaacatagc
     7441 tacatactca attaatgtct caatagaaag acctgcacct ctcatacact ttatgaaatt
     7501 aacccactca caatcttctt ctgtaaaaga cctaattcca cttttatttc tatttacagg
     7561 tggtattaaa ccaattcgtt cataatatcg taaagtatcc gctgaaagct cgtatttttt
     7621 actaacttct gaaatcgtca tacatatctt ctcctcttga tattgtatag attgaattat
     7681 ttctcaaagg tttctgctac attctttaaa gcatctataa ttttaatgtg ttgaggacag
     7741 ctttcttcac attgtttaca ttcaatacaa tctgaggcct ttccataagt tttagtataa
     7801 ttattatagt aaaccatctg tgttgaaaat ccagtattaa gagcttgttt ttctgcattg
     7861 tacagcgcaa aatagtttgg aatagcgata ttttttggac agccttctac acaatactga
     7921 caggcagtac aaggaactaa aattgattcg ttgataatct ctactgcttc atctataata
     7981 ttatattcct cttgtacgaa tggtttgaaa ttttgcatat atcctgtatt gtctaataat
     8041 tgttccatat ttgacatccc acttaagacc atcataacat tatctttact tgctgcaaaa
     8101 cgtattgccc aagatgaaat tgacatatct ggattgtagt cattcaataa tttctctgct
     8161 ttttctggta tctttgcaag tgtacctcct tttactggct ccataacaat tacaggctta
     8221 ttatgcttct ctgccacctc gtagcatttt ctagattgta tgctttcatt atcccaatct
     8281 aaataattaa tctgtaattg cacgaagtct acttctggat gctcactcaa gactttatcc
     8341 aatagttctg cactatcatg aaaagaaaaa ccaatattct ttatttttcc ttcttttttc
     8401 ttttcttcaa taaacttaaa gctatcaaat tttttagcta cttcataatg tgacacgcct
     8461 atattgtgta aaagatagta gtcaaaatag tctacaccac atttttctaa ttgctcatta
     8521 aaaatggttt cttgctcttc ttttttcttt aaagccatta atggaagttt tgtagctata
     8581 gtaaaactct ctcttttgtg tctttttact agagattctc taagtgctat ctcacttttt
     8641 cccatgtgat acacataggc tgtatcaaaa tatgtaaatc ccctttctaa aaaagtgtct
     8701 accattttat ttaaatgttc catatcaata gttgtaacat cattttcatc ttttattggt
     8761 aaacgcatta atccaaatcc taattttttt gcctccattg ttttacttcc ccctttaaaa
     8821 cttgtttgtt ttttctctct actttaacta ttcttacaag tatataattc ttgtataatt
     8881 atactttact acttggagtt aacttcaagt caatagattt ttttattttt aagacaaata
     8941 ttataaaaat acagttcttt aatattaatc cattgcatgt ttctttcacc ctccaattct
     9001 caaacttcac aaactccaac atgcaaagaa atatatactt taagaaattc agtccattaa
     9061 tattactaca aatacttttt agatatgttt aaaaagaaat ttcacctaaa atatttataa
     9121 aagatttaga aataggtctt gtgtctattt taaaatatat aaaacagtgg taaagactta
     9181 tttataagat aaaaaatgaa tagtgtatac cctttttaaa aagttttttc ctttttatat
     9241 ttgtgataca atgttaaata taaaaaggaa aattttgtat taaagaaatc ttatttgttg
     9301 attttgtaac ttcaaactca gtatcaattt gattttaaga ttatatttta ttaagtatgc
     9361 taaataacaa atttttaaag attgtgaggt aaaaatatga ataataaaaa tttagctata
     9421 ttaatatcag gtatgatgtt tttttctagt tcttcagcaa tttttgcaga caatactaca
     9481 aagcaagaaa gattaatagg taaaacaaga tatgaaacag cagtagaagt aagtaaattg
     9541 ggatgggttc aatcaaaaac agccattata gttaatggta attctattca aagtgcgctt
     9601 tgtgcaaatc catttgctaa gctaaagaat gcacctatac ttttagtaaa caacaacagt
     9661 attgaaaatt caactaaggc tgagcttaaa aggcttggtg tagataatgt gtacattgta
     9721 gatagtggaa atagcatttc ttctaaagta gaaaatgaaa taaaatcact taacataaaa
     9781 ataaataaaa tagtaggaaa caatatatat aaaatgtcta caaatgtact taaagaaata
     9841 gataagataa aaaaggtaga aaatgttgca gttgtaaatg gactaaaggg attatctgat
     9901 gcagttagta tagcttctcc agcagcaata cataatatgc ctataatatt agtatcagat
     9961 gaaaacagca atgatagtag tataaatttc ttaaacaata ggtctataaa aaaatcttac
    10021 atagttggag gaaataagat tttatcagac aatacagtaa agaaatatcc aaacagagaa
    10081 agaatcagtg gttcagatag aaacaatact aatgcaaaaa ttattgataa attttataaa
    10141 gataaaaaaa taaataatgt atttgtagct aaagatggta ttaaccaaga aaatgcatta
    10201 gtagattcat tatcaatagg agtattagct gctaaagaaa attctcctat aatgatatct
    10261 aatggtagcc ttgtaaatag tcaaaaggaa tttattaaaa ataaagatat agatgaaata
    10321 acacaagtag gaggaggata taatcaaaaa gcatttgaag atattttaaa actaaaaaat
    10381 gtgaacataa gaaataaaac taaaaaaata tctttaatac aggctaaaga aatattaaaa
    10441 caagctcttc caggaagcga tattttagtt tgttattatg atgaagatga taatgaatat
    10501 gactgcaaag ttataaagga taatatccta tatgagatag aaattgatgc ctactctggt
    10561 aaaataatag aaattgaaaa agatgatgac ttaaatgata ataatttagg tacacaaaat
    10621 atatcattgg ataaagctaa gtctatagca ttagatgctg ttcctaatgg taatttaatc
    10681 tattgtaaat atgatgctga agataacgaa tatgaggcta aagtaataaa aaataagaaa
    10741 acatatgaat tagagataag tgcatttgat ggaagaattg tagatattga catggacaat
    10801 gattctgatg atgactttga tgataatgat aagaataact ctaatactaa tgacaacagt
    10861 tctattatta gtgctgaaaa agctaaatca ataatgctag ataaagtgcc tggtgggaaa
    10921 atagtaaaat ttaagtatga tgaagatgac aaagaatatg aaggtgaaat aataaaagga
    10981 aaaattgaat atgagataac aattaatgca attactggta aaatattaga atttgaaata
    11041 gatgattaat aaaaaatata tattctatta attattacaa gcaataaagc cataaatatt
    11101 gaatttttca atatttatgg ctttatttta ttttttttaa tttaaatcta cattatgcca
    11161 ttatatatct atgcactctt ttaaattaaa atcttgtcca tcatcccaaa cctaataaag
    11221 ttagatttta aacaatatat tgtgatataa gtaatttgtg tagaattatt gcgtgattta
    11281 ctcatgaaga aacaataagg ttaatatata atttgactat attaagacga aaggagacat
    11341 atacaatgaa tacaatatta aaagtaaata acataactaa aacttataat aaagggcaac
    11401 agccgagttt agataaggta tcctttaatg tatcagaagg tgaatttata ggtataatgg
    11461 gagcttctgg ctctgggaaa acaactctgc ttaatgtatt atctacaatt gataaaccaa
    11521 caagtggaga tattttcata aataatataa atttgaaatc gcttaatgac acttgtgctg
    11581 ctgattttcg taaggataat ttaggcttta tctttcaaga gtatttttta ctagatagtc
    11641 ttacaatttt cgagaatatt tcagtacctc ttacacttca gaaactcaat ccagataaaa
    11701 tagaaaacat ggtaaagagt ctagctgaac gatttgacat agcttctcaa ttaaataaat
    11761 acccaaatga attatctggt gggcaaaggc agagagcttc agcagttaga gcaatagtaa
    11821 aacatcctag tattctcttt gcagatgaac caacaggagc tttggattct aattcagcta
    11881 ctgaactact ccactcacta caggaagcaa atgaggagtt gaaaacaaca attttaatgg
    11941 taacacatga tgcctatgca gcaagttttg caaatagaat tttgattttt aaagatggtc
    12001 atatcattaa agaattaaat aaacaaccga ataatagaca tacattttat gagttgatta
    12061 ttcaagaaat tgcaaaattg gatactaaaa gataggagga taatttaatg aacatttttt
    12121 atatggcaat aaagaatctt aaaaaaaact tttcattcta ttctctatat ctactttcag
    12181 tctcttttgt tattactgta tttttcgcat ttacttcatt ctctgttaat aagataatgc
    12241 tagaaaaaat ttcaactgat ggtagagttg aatctatgtg taatgttatt tcgatatttt
    12301 tgatggcatt tgtcatcttc tatatggcat actcaaatcg attcttcttg agacgtcgta
    12361 caaaagaatt aggcatatat gctttacttg gctatcgtaa gtttacaata ttatcgctac
    12421 ttacctttga aaatgtatta atttgtagtg gagcttttat tactggattg gtattaggag
    12481 cattgtcaca taagggtatt gtctttggaa ttacagcact acttaattta tcaattgata
    12541 attcacagat accatttttt aatataaatg caataaacaa aacaattatc tttatagcat
    12601 tagttgtttt agtcttagct atatcaaatg gtaaatttct ttataagact tcacttgtaa
    12661 aattgataag acatgaaaaa agtgcagaaa aaaaattaaa gttcagaaaa attccagcct
    12721 ttatgggatt tattatgatt ttaagtgggt attgtttatc actagatatt cttcgtggag
    12781 ctaaatcagt atgggtaagt attggatttt atcaaatggg cttgataact atgattatca
    12841 ttgtaattgg tacagttctc tttatcaatt cctttcttcc atatattatg caaaagagta
    12901 aaaaaaataa acgtactttc tacacagata tgaagataat agctattcca aactttatct
    12961 atcgtatacg ttcaaactct aaaactctaa ttatgcttac attactctct gctactactt
    13021 tgacagtttc aagtgttatg gcactgtcct tatactaccc tattgcatct gtttcgcgta
    13081 ttgcaccttc agagatagaa tttagagtag aaaaaaataa acaaatagaa gatgtaaaac
    13141 aaattgtcag tcaatatacg tctaatacag atgatattat ttttacacag acaaatattt
    13201 ataaggtaaa atcttcatca aaagaccttc catttgaata tgaaattggt acatctaaga
    13261 ataatttatc taatagtagt tcttctaaag aagttatttc aagagaatca ggatttgaat
    13321 gtatatcttt ttctgattat tatactttat taaaggcaca aaatcgaaca aagctactaa
    13381 aacaacttac ccctttaagg gataatgaat gtattttaat gaaatatcaa ccaaataatg
    13441 atggatatag tgaaaatgga acgatttatc cattacttat taatggtaaa gaaacacctg
    13501 tagctgtaag ggaaacatca ttaaacaatg ctatttcttt tgccaatagc gttggaacac
    13561 ttatcgttaa tgatagtata tatgagcaaa tagcacaaaa taaattacca atgacaagca
    13621 ttttaagtat taatggtaaa tcaattaaaa ataatgaaaa tttatatagt aatatttcag
    13681 attatttaga tggaagccct tatttacaag gatattctca caggataaat gatttattat
    13741 caattaatag ttctacattt ctgttaatcg gatttcttgt ggtattattc tttattgctg
    13801 ttggaagtat tttatacttt aacaatatat cagcaatttc agattcaaca tctgattata
    13861 agattttaca gaaaatgggt tatgataata tacaaattaa aaaaattatt aaaaaacaag
    13921 tattgccatt ttttaatatt ccgtttttac ttggtatatt ggactgtata tttgctacct
    13981 tggtttataa gactgctttg atgcagaatt tacttggaaa ttcattttca caatacatac
    14041 ctgtgattat tgcaattttc ttaacttctg tagtgtattt gatatattat atacttacta
    14101 ttaaatcctg ttgtaaaatt gtattaaaaa attaatattt accaaatcgt caagcttaga
    14161 ggtgtgacga tttggtatac ttcatttata agtatttgat ataatttaaa attatagatt
    14221 cttaggtttg atttagtcat aaaattaata taattaactc ataaggaggt gtataagatt
    14281 gacaaatttt aatttaaaaa tactattttt aggaaactac attctatcac ttctcttcat
    14341 gggaatatct acaagcactg aaacttatat gaatatccaa agcattattc ccttaataat
    14401 ttcaacatta ttactcatag acatcacata tataacctct tgtaatgtcc gagaaaataa
    14461 cattatttca ttattttgtg gattactagc actagacagt tggtatttac tcttatcatt
    14521 tgtaaccaca ccaataggca ctattatttt taaaatatta agcacagtta tagtgtatgt
    14581 ctctattaaa tttatatttt tatttttgtt tcaaggtagt ggatacaatt tccgtaagat
    14641 aacagatatt ttattgctta tcacctgcat tggtgcaatt gtaggagcat ttatttctaa
    14701 cagaacgtat gcttgtatgt acggaataca gtttgttgtt agtataataa gctttttata
    14761 tattattttt tatcatttga aaagggttgt ttttgtcata aaaagcgaaa aaaagcctat
    14821 atcgatttca gtatttataa caattatagg attccttact tattattttt ttacactaag
    14881 aatatcaaat catattggta attttggaat ctatctaact gtattggtat tttttatgag
    14941 tattcatggt attgcactta aagaaaatga tagttttcct atttcaacag tttttaacat
    15001 aaaacaaagg gctatatttc tttttataag tattttagta attagcttgg tcgtactatt
    15061 tgccaatggg aatttttcta taatattaat agctataaat gttttattta tgcttatttt
    15121 cctctgtaac attattttgg agtttaacct tagagatgaa aaaaataaag tagttaaaaa
    15181 tagcaagtat acttttgcat tagaaaaatt acaacaagaa gaacaactaa aaacagagtt
    15241 ttctaatttt ttacatgatg acatattaca gaacctgctt tcaataaaaa acatgatgag
    15301 taagtcatat cgcccagaag ttcaagatat tatatatgaa acacttaaca atctaaatac
    15361 atgtataaga aatcaaatgc aagactatca tcctgttatt ttaaaaaatt taacagtaaa
    15421 agaaaattat cagaatttaa ttgaaagtgt ttcttcatcc tttccacaaa aaaacatttg
    15481 tgtttcattt aagtgttctg atactttatt cttagttgaa ccatatgata tattgatata
    15541 tcgactgatt aaagaattat tgacaaacgt gtataaacat tcaaatggta gccatgcatg
    15601 gatagcttta gcactagaaa aaaatataat aaaattaaat attaatgata atggtgtgaa
    15661 aaatacatct tttaaaacac ctttagatat aactaaaatt acttttgata taataaaaca
    15721 caaagggatt atatcaatta aagaacaagt agaaaatcta ggaggaacga taagtatttc
    15781 tgataatata cctcatggag tttgtgtaca gattacaatc cctatgaaag gagatggttc
    15841 ttataaatat tttgttagtt gatgaccata tgctttttgc taaaagcttg tcaattgcac
    15901 tagaagaata cgaagaaatc gaacagtttt attccacaca ggacatccat aatctttcag
    15961 aaatgattca agataaaaaa atagacattg tcttaatgga tataaacctt agaggcttaa
    16021 gtgaatgtga tggcttaaaa gtagcttata atttattaga gactaattca actattaaaa
    16081 taataatctt gactggatac gacctcccag tttataaaca tgaggctaaa aaaataggta
    16141 tctatggatt cctaaataag aatattagcc ctgatagttt aattgattca ttattaaaag
    16201 tctatcaagg ggtaacctgt tttccatctg aaaatactga agtaattatt gaagatttaa
    16261 ctaatatgga gaaaagaatt ttacagctta tctgtagtgg taaaaaaaga aaaataattg
    16321 ctgatgaatt gtatataagt gaaagaacat tatcaaatca tttacaacac atatatgaca
    16381 agctagatgt ttcatcatca gttgaggcta ttacaaaagc cattaaaatg ggttatattt
    16441 ctccagtata ttaaaaaagt gagtgtctca aaatgaactt tctagctcat aaggcactct
    16501 cctttgtatt aaatcaaata tattttcatc atttaaacaa ttaagaagag gcttctctct
    16561 attttgagac aactccattt taatacacta tttatattta tatataaaca tattttaaac
    16621 aactaaaaag caactaatat ctcaatacat atcttcataa cacatatcac cataattaca
    16681 agcagataaa gaactctcct catttaaata cgactcaaat tcatacccaa acagaattgt
    16741 tggttctata tatatttcca tatctgtata tctccatctt cttacctttg cctctataac
    16801 cttgtaaaaa tcttctaaac tatacccttc ttttaatctt ttatctataa gtgccttagt
    16861 tatactcaaa tcagctttaa aattttttcc tgattcacta tttaattttc ttataataat
    16921 ttcatgtatg ctttctggat ttttataagt agaatcttca gtcaaatctt catccaaatt
    16981 ttcaaaggta tttttattta aattcttttt atttaaatta ttttttaata attctttttt
    17041 ctttgtctca ttttctgttt catacccagt atgaattatc aaatcataat ctttgtctat
    17101 actctcttga tttatacggc tgtatgtgtt ggtattactg ttcttgaaca actcatctct
    17161 atgtttatat ttcattgttt tttggttatt gttaatagtt gcatcaatgt atttctctat
    17221 atttttgaaa atatcactat tctttgttac aatttctgca ttactatttt cagaacaaac
    17281 ataactatat acagaacaac aatttccctt tactccccta cttattaatc tcaatatacc
    17341 taaatctata aattcattta caagtctact tattgtagat atagatacat caaggtcaga
    17401 tgcagctttc cttactgtca tcataaattg tcctcttggc agagttcctt ttaatctttg
    17461 tatattttct tttctcatta catagtgatt aaaccttatt ctatttacat ctcttttttc
    17521 attaaaatat aatactgctg aatttaattg ataaaaacta ttactcatat tcccactcct
    17581 ccaatttaaa tagataattt gttgtaaagt ttttagattt ttaaataact tattaaatga
    17641 ttatttttat taaaaagtat tctttgatat tttcatttta aagttttaag tataataagt
    17701 taatagtgta gatttaactt ttttatttaa acaataaact taatattatt tttaatataa
    17761 taattttatt aaatttagta tgtaattttt taatcttatt ttattccctc tatactctta
    17821 tgctaaataa taactttgat aatatttccc taacttatgt ttcaactggt ttatattgca
    17881 atttatttaa ttacagctta tttgcataat aattcgtcca tatcataagt tgtgtattta
    17941 tataaataca caattaaaaa taaatcacta tggtatacta attattacga agactatact
    18001 tatatgcaaa aataaatata aggcatctta tatcttttat taattataaa tatcttttat
    18061 caattataaa aaattaaacg ctatcttatt tatcataaat tgcaaaaaca taaaatttta
    18121 ttactatatt gctataaatt aattaataca cattttacac aataaggagg gctatatatg
    18181 acaaatgcta aaagtaaaaa agaatatcta aatcgtattt gcaaagtgca agactatata
    18241 gaagaacatt tacatgaacc tctttctctt gatgaacttt ctaatatagc aggtttttca
    18301 aaatttcatt ttcatagaat cttcaaagca atagaaaaag aaactttggc acaatatgta
    18361 aataggctaa aactagaaaa tgcaactgct tttcttattc atcgaactga catgactgtt
    18421 actgatattg cacactactt cggctttact gattcagcag ttttctcgag agcatttaaa
    18481 aattactata aaataagccc tataaaatat agaaacaatt atagcaagaa ttgcaaagaa
    18541 ccatataaaa tttctcagta caatagaagc atatcaaaac ctaaatgtaa gaatactaga
    18601 gaggttaaag gtgaaattga aattctagaa cttgataata tgaatactat ttacactaga
    18661 tatactggtt catatggtaa tctaaccagt actttcccaa aacttttaga acggttattc
    18721 acatatgcaa gtgagcagaa tctacttgga cttgaaaaca cacaaatatt agccatttat
    18781 catgataatc cacaatttac acaggagcat caattaagga ctagtatttg catgaccatt
    18841 ccagataaca ttgttgtcaa agagaatgac aatcttggca atatgataat cccttctgga
    18901 aaatatcttg tgggacattt tgatatttca aaatctgaat atagtgatgc gtgggatttt
    18961 atgtataatg aatggctatc aaatggtaat tataagctac gtgcctcctt cccttttgaa
    19021 gtatatctta acaacccaca caatcaccct caaaatagac actttgtcga tatatattta
    19081 cctatagaac ctttttaata acctcaccta atctattctt gtattaagga ttaatatatc
    19141 cctattttac ggtttgataa aattgataaa atgaggtgaa ccaatgaaag aaataaaaat
    19201 taatggagca aagatacaca atcttaaaaa tatagatgtt tctatcccca aagataagct
    19261 tgttgttgca acaggtgtca gtggctcagg taagtctagc cttatgtttg acattgtatt
    19321 tgaggaaggt cgtaaacaat atctgcaatc tttaggtata cttgcaggta ttgatagtga
    19381 ggataagttt gataatattc aaggtttagc tcccactata gctgttcaac aaaatattat
    19441 tcgccagagc aatcctcgtt caacagtagg cacaagaacc aatattctca acatgcttgc
    19501 actattgtat tcagtagaag gtcaaataat gtgtactatg tgtgatactc ctgtaagcga
    19561 taatctaatt tgcaataatt gtgggtatga ggaagaacga ttaagaccaa gttatttctc
    19621 atacaactct tcagatggaa tgtgtatgaa gtgctctgga catggtgcat attttgagat
    19681 taatattgaa aaattagtcc ttgataagca tgatacccta aaacaagtct tagatagagc
    19741 aaagattaca ccaggatatt tgagagtatt ttctaaaaaa ttcaacgatt atttagacat
    19801 acaattctta caactacctg aagaaataag aaatgaagta ctctatggtc attatgaaaa
    19861 tggtaaaaaa agttcctctt tatcaaaggt atttcacaat cgttacaaaa ggggtgagga
    19921 tttaaatgga gtttatacta tgactgcctg ttcagattgc aatggtttta aaattggtga
    19981 agaagcacgt agagtacttt taaacggaaa acatattgga gaacttggaa aaatgacgat
    20041 tttagaaatt gatgatttcc ttaaaatctt aataaaccaa ggtaatttaa acacatttgg
    20101 tacaaatctt gtaaatgata ttttatccaa aactcgacat ttaatagaat cacgattagg
    20161 tcatctatcc ttataccgag aaatgtcaac tttgagtggt ggggaaatgc aaagactatt
    20221 tttaaattca catctcgatt cagagatgga ctcattaatt tatgtgcttg atgaaccaac
    20281 agctggattg catgaatcag aaaaaataga catcttaaaa tcaataaaga aacttaaaga
    20341 tttaggcaat acggtcatag tagttgagca cgataaaaac acaattgaaa tggcagaaca
    20401 tattattgat attggtccaa aagctggtgt tgaaggtggt caattagtat atcaaggaga
    20461 tttagaaggt ctacttcaat ccgacaaatc acttactggg cagtatctat ctggaaaata
    20521 tccaatgcct aatagaacaa gcaataaaaa tattacacat actgataaaa taccttgtat
    20581 aactattcaa aatgcaaata caaataatct taaaaatgta actgtatcat tacctttggg
    20641 tgctatggtt ggaatatctg gaaaatcagg tagtggcaag agttcactta tatcagacac
    20701 cctacttcct cttcttagaa gccaatttaa taaccatact atcaataatc aaataaattc
    20761 ttcagatagc gaaataggtg atgaagacga ttatgcagtt attgaaacta ttgcagatag
    20821 actgattggt actaaacata tttctggata ttcagagata tctcaatctc ctattggcaa
    20881 aaatatgaac tcaactcctg cttcttatat tggtatatgg gataagatac gtacactatt
    20941 tgcaggtcag ccagaatcgt tagaacgagg atttacagct ggtcatttct catttaactc
    21001 caaaggtgct tgtcctaaat gtagtggtag tggatatgaa aaggtctggt taggaaataa
    21061 tctaagtata gaccatatat gcagtgaatg tcagggaaaa agatttaatg atgaatcttt
    21121 atctattaaa tataaaaata aaaatataca tgatatttta aatatgagtg tttctgaatc
    21181 tgtacatttc ttcaaggata ttccaaatat tgtttcacac ttaaatgtat tagaacaaat
    21241 aggtatgggg tatataaaat taggccaacc tacaccgact ttaagtggtg gtgaatccca
    21301 aagaatcaaa cttgcaaaag aaattggtaa aaaacgaaat ggtaatatcc tttatgtatt
    21361 ggatgagcca actactggtc taagtctata tgatacttct aagttgattc aattattaga
    21421 tgaattggtg gaaaatggaa attctgtaat tgttgttgaa catgatatag atgtattaaa
    21481 tgtttgtgac tggattgttg agcttggtcc agaaggtggt gacaaaggag gttatataat
    21541 tgcagaaggt tctccaaaaa ctctaaaaga aaatccaaaa tctataacag ggaggtatct
    21601 ttaatgaata aaaacaattg cttagattta atatttgaaa aagtagaccc ttcaagtgtt
    21661 ggcatgagtt cagaaaagct ctcacaactg gaacctttaa taatctcaca atatagtaat
    21721 ataaatggaa taattattgc aagaaaagga aaagttgtct ttgaaaagta ctataatgga
    21781 tataatgctg atgatacttt tcatgttgca tcagttacaa aaagtattat atcagcctta
    21841 ataggtattg caatagataa aggatatata aaagatgttg accaaaaggt tgttgatttt
    21901 ttccctgaat atacttgtag tccttctgaa atatgtaaaa aagcagtaac tatacgtcac
    21961 ctacttacaa tgactgcacc ataccctttt aaaaatatga ctgagtcttt agaaagactt
    22021 tgcaaacaaa aagattggat aaaatatacc ttgaatcaac ttggtcaaaa aggcgaaatt
    22081 ggtactttca aatattcttc agctggaacg cacctacttt cagctattat aacaaaaact
    22141 actggaaaat ctgctcgtga atttgctaat gagtacttat ttaaacctat tggtatgaaa
    22201 gttataccag attatgatat gaaagatttt ggttttgaag atttctttgg aaaaaatctt
    22261 aagggttggg caaaagatag acagggttat tctataggtg gctggggact tacattgagt
    22321 gctagagata tgttgcgatt tggttttctg tatttaaatg atggtatttg gaataataaa
    22381 cgtattatat ctaaatcgtg gattgaagaa tcggttaaaa tgaatagtaa tgaatacggt
    22441 tatctgtggt ggatatgtaa aaaagatggt gttttgtggt attcagcaat gggaactgga
    22501 ggaaatgcta tttgttgtat tccacaatat gacttagtaa tagccatacc atctaaaata
    22561 gtgtttaaac ctcgtgatag atgggagttt attgaaaaat atatacttac ttctataata
    22621 gattaatcac tcttagataa actaattact cttagataac caaaccctcc actataacac
    22681 cagacttttg tttggtgtta ttatgactaa tactatatat ctaaaaggag ttgaatacta
    22741 atggacactt acacgacatc agaggtagca gatttaattg gtgtacatcc taatacagta
    22801 cgtttatatg aagaactaaa cttaatacct aaacctaagc gtaataccaa tggttatcgt
    22861 atctttaatg attttcatat agaacaattt agatttgctc gtacagcctt gaaaattgaa
    22921 gtggtacaaa ggggattaag aaaagatgcc cttgagattg taagaacatc agcgaatagg
    22981 gactttgata aggctattag tcttgtaaat caatacttgc aacaattaga aatagaaaaa
    23041 tctaatgccg aggaagcact tactattgtt gcacagattt taaatggaaa tactccagaa
    23101 acaaataaac tctttttaac tagaaaagaa actgcagaac acttaaatgt tacaatagat
    23161 actttaagaa attgggaaat gaatggtttg ttaaaagtaa aaagaaaaca aaatggttat
    23221 cgtgtatata ctgatgaaga tataaaacga cttaaaatta taagcgcact tcgttgtgca
    23281 aactattctc tttcagctat tttacgaatg ttaaatgctt tatcttctaa tcctaatact
    23341 aatattaaaa ctgtccttga cactccaaaa gatgatgacg aaattatctc agtgtgtgac
    23401 aaactgataa cttcattgaa agaagctgaa cataatgcaa aaaccatgaa atctcatctt
    23461 caaattatga aaaaacaatt ttcctagccc tccactctaa ctccagactt tgatttgtgt
    23521 gttatcctta agagcaagaa aaattcaaca cacaaatcaa aggaggaata ggatggaaaa
    23581 caaaaatact aattctacat ggaagaaaaa agtcattctt tttttagcaa gtcaatatat
    23641 ttccttattt ggctcatcac ttgtacaaat ggcaatgata tggtatgtca cacttcagac
    23701 atcatcaggt gtatgggtaa cagttttaac tgtatgctcc tttatacctc aaatgataat
    23761 ttcttttttt gctggtgtat gggcagacta ctacaatcga aaggtactca taattgtatc
    23821 agattcaaca attgctatat ctacattagt tctaattcta tttatgatgt catcaaatgg
    23881 cacaacaagt gaattaatag ccatatcagt aatatctgtt atacgttcac taggaagtgg
    23941 tgtacaaaca cctgctgtaa acgcaatgat tcctcaactt gtaccagaag aacatttaat
    24001 gcgttttaat ggtataaata gtagtctaca gtctatagta caatttatag tgcctattgt
    24061 agctgctgca atacttggat ttggtaataa aataaatcaa gttcttttaa ttgatgtatt
    24121 tactgcaatt attgctattg tattattgat attcatcaaa attcctaaac ataatatacc
    24181 aaaacaaact gaaaaagtct cattttttgc agatatggta tcagggataa agtactgttt
    24241 ttcagataag ttcattagaa aagtgctttt cacttatgga gcattcatgc ttctttgtgt
    24301 tccatcagga tttcttatag gacttcttat tgaacgaaca tttggcaagg gttatatata
    24361 cctgtctatt attgaaatga ttggattttt agttatggtt ttaggtggat tgattatagg
    24421 agtttttggt ggttttaaaa atagaaataa gacctttttt ctagcaatat tatcctatgc
    24481 tattttttca attattctag gtcttgtaac acaagcttgg caatttttta tatttatgtt
    24541 ttttacgaat ttttcaattc caattattca agcatcagct atgactatgt tgcaagagca
    24601 tgtagcacca caaatgctag gtcgagttct tagcttacct attattatat atactggttt
    24661 tattcctctt ggtatgatga ttttcggacc aatggcagat gtaatttcta ttaataattt
    24721 aataatcgta gcagggatta tacttttatt ttttgctttg gctatcccct attcaaaaca
    24781 attttacaag caaggaatat ctaagtcaaa tgaatccgag taatatattt aaatagttta
    24841 actcgattta tctggttaaa aatgaggggg cttaaaatgg actttttaat ttatgaagaa
    24901 aattttttat atgaaatcaa atatatttta tcatttcaac attgaaaaat aggtttatct
    24961 ttattttgag tccctctctt tatagcaaat aatattttga tactatcttt attaacttta
    25021 tataataata cctcatataa taatacctcc atttatatca taaattaaat ttatagtaca
    25081 aatggaggaa aataaagtat ttatatagtt taagtaccta ttaa
//
LOCUS       DAGQWR010000055        24602 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.055, whole genome shotgun sequence.
ACCESSION   DAGQWR010000055 DAGQWR010000000
VERSION     DAGQWR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24602
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.055"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            118..1305
                     /locus_tag="MIW95_001889"
     CDS             118..1305
                     /locus_tag="MIW95_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBY3151217.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYNGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            1321..2904
                     /locus_tag="MIW95_001890"
     CDS             1321..2904
                     /locus_tag="MIW95_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBY3151218.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            2932..4260
                     /gene="ssnA"
                     /locus_tag="MIW95_001891"
     CDS             2932..4260
                     /gene="ssnA"
                     /locus_tag="MIW95_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBY3151219.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            4459..5811
                     /locus_tag="MIW95_001892"
     CDS             4459..5811
                     /locus_tag="MIW95_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBY3151220.1"
                     /translation="MSQDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            6003..7112
                     /locus_tag="MIW95_001893"
     CDS             6003..7112
                     /locus_tag="MIW95_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY3151221.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            7395..8786
                     /gene="hydA"
                     /locus_tag="MIW95_001894"
     CDS             7395..8786
                     /gene="hydA"
                     /locus_tag="MIW95_001894"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBY3151222.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            8780..11341
                     /gene="xdh"
                     /locus_tag="MIW95_001895"
     CDS             8780..11341
                     /gene="xdh"
                     /locus_tag="MIW95_001895"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBY3151223.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            11493..12839
                     /gene="rpoN"
                     /locus_tag="MIW95_001896"
     CDS             11493..12839
                     /gene="rpoN"
                     /locus_tag="MIW95_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBY3151224.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            13636..14658
                     /locus_tag="MIW95_001897"
     CDS             13636..14658
                     /locus_tag="MIW95_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBY3151225.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            14718..15725
                     /gene="gap"
                     /locus_tag="MIW95_001898"
     CDS             14718..15725
                     /gene="gap"
                     /locus_tag="MIW95_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBY3151226.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            15947..17149
                     /locus_tag="MIW95_001899"
     CDS             15947..17149
                     /locus_tag="MIW95_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBY3151227.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKANNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            17175..17918
                     /locus_tag="MIW95_001900"
     CDS             17175..17918
                     /locus_tag="MIW95_001900"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBY3151228.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLSK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            17927..19459
                     /locus_tag="MIW95_001901"
     CDS             17927..19459
                     /locus_tag="MIW95_001901"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBY3151229.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFANKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
     gene            20047..21339
                     /gene="eno"
                     /locus_tag="MIW95_001902"
     CDS             20047..21339
                     /gene="eno"
                     /locus_tag="MIW95_001902"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBY3151230.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTERLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
     gene            complement(21459..21887)
                     /locus_tag="MIW95_001903"
     CDS             complement(21459..21887)
                     /locus_tag="MIW95_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBY3151231.1"
                     /translation="MNPVKELNNLWHIVSMQMKTEMCSGKYPTIHGISLVELSILEIV
                     EQYPNSMMKEISEILELPKSTLTSAVKRLELKKFIKKNPCIKDKRRYNLVLTELGLKA
                     QQEHRDIEIIVFRNLLHQLDKDEIETFIRIFSKAVNKSKK"
     gene            21972..22478
                     /locus_tag="MIW95_001904"
     CDS             21972..22478
                     /locus_tag="MIW95_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3151232.1"
                     /translation="MNLEIIRATSENAIEMGFIHSCSWRKAYKGIIPDKIIDEFTSEK
                     RAEVFQSVIPKAEEEYYLFKVNGIPAGFASLNRSQEDNAPKCVGEIYSIYFHPDFWGT
                     SVTKKGLQFCIDRLSNLGYSYITIWVLKDNTRAINFYKKNGFTCDDFEKEIHIGKKLL
                     EVRYSKKI"
     gene            22596..22820
                     /gene="secG"
                     /locus_tag="MIW95_001905"
     CDS             22596..22820
                     /gene="secG"
                     /locus_tag="MIW95_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBY3151233.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(23204..24142)
                     /locus_tag="MIW95_001906"
     CDS             complement(23204..24142)
                     /locus_tag="MIW95_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBY3151234.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIRH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKTIILGVCGKLILVPSIFLPISIFLGFRNVELTSLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(24130..24528)
                     /locus_tag="MIW95_001907"
     CDS             complement(24130..24528)
                     /locus_tag="MIW95_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY3151235.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSEKYKLKARNVK
                     INLNIKGYKFLPYVKNLKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
BASE COUNT         9759 a         2855 c         4740 g         7248 t
ORIGIN      
        1 aaataaaata taaagaaata aaaaatcaat agattaagtg aaatgctaat aaattaaaaa
       61 agtgaaatat caaaataaaa cgtcaacaaa ttaaaaatta tatgggggaa tatcaaaatg
      121 ttaaatgaaa caagaaaaaa acaattaact gaagtatgcc aagacttaat aagaaatgct
      181 agttattcag gtcaagaaga aaacgtagta aaagctatag aagaaaattt tgctttacta
      241 ggttttgata gttggtcaag agatagatat ggaaatatag taggatgtat aaaaggaaat
      301 aaaccaggta aaaaaatatt atttgacggg catatagata cagttccagt tccagatgca
      361 agtaaatgga gtgttcctcc ttttgaagcg agaatagtag atgggaaaat atatggtaga
      421 ggaacttctg atatgaaggg gcaagtaagt gctatgatgg ctgctgtttc atactttgca
      481 gaagatacta acaaagactt tgaaggtgaa ttgtatgtag caggtgttgt tcatgaagaa
      541 atatttgaag gtgtttcagc tagagagata agtaaggctg ttcaacctga ttatgttgta
      601 ataggtgaat cttcagaatt aaacttaaag ataggtcaaa gaggtagagg agaaatagta
      661 gtagaaactt ttggtaaacc agctcactct gctaatccag aaaaaggtat aaatgcagtt
      721 tataaaatgg ctaatgtaat ccaaaagata caacaattag tacctccaac tcatccagtt
      781 ttaggtgatg gaatattagt tttaacagat ataaaatctt ctccatatcc aggagcatca
      841 gtagtgccag attattgcaa agctactttt gatagaagat tattagttgg agaaacaaga
      901 gaaggcgtat tagctccaat acaagcttta ttagatgaaa tgatgaaaga agacccagaa
      961 ttaaatgcaa aagtttctta tgcagttgaa aaagctgatt gctatacagg aaatactata
     1021 gaaagtgaaa gattcttccc aggttggtta tatgatgaag aagatgaatt tgttcaagca
     1081 gcgtataatg gattaaaaga agctggaata gattcagaaa taactcaata ctcattctgt
     1141 acgaatggaa gtcactacgc aggtgaggct ggaataagaa ctataggatt tggtccttct
     1201 aaggaaaatc ttgctcatac aatagatgag tatatagaat tagaccaatt attcaaaggt
     1261 gcagaaggat attatggtat attaaaatca gtttataaaa aataattagg agatggtttt
     1321 gtgagaactt taataaaaca tgggcttata gtagatggaa ataaaacacc agcatatgaa
     1381 ggagatattt taatagaaaa cgagaaaata ttaaaaatat cacaagattt aaacgaagaa
     1441 gctgacaaag ttatagatgc aaaaggtaga gttatatgtc caggtttcat agatactcat
     1501 agccattcag acctagttat tttagttaat ccatataatg aggtaaagat tagacaggga
     1561 attacaacag aagtgttggg ccaagatggt atatctatgg cccctctacc tcaagagcat
     1621 atatcttctt ggagaaaaaa tttagcaggt ctagatggtg aaagtgatga aattgattgg
     1681 aaatatgaaa caacagagaa ttatttaaaa atgatggatt acaatggggt aggtttaaat
     1741 gaaacttatt tagtacctca tggaaacgtt agaatggaag ctatgggact tgaagataga
     1801 cctgctacta aagaagaaat tcaaaaaatg tgtgaaatca cagagagaga attaaaagca
     1861 ggagctatag gattgtctac tggacttata tatatacctt gtgcatactc attgactgaa
     1921 gaaattatag agatgtgtaa ggtagtagca aaatatgacg gagtatttgt agttcaccaa
     1981 agaagtgaag cagatacaat tttaacttca atggaagaaa tcatagaaat tggaaaacaa
     2041 tcaggagtaa aagttcattt ttcacatttt aaggtttgtg gtaaagcaaa ctggaagtat
     2101 ataccacaag ttatagagct tttagaaaaa gcagaaaagg aaggaataag agtatctttt
     2161 gaccaatatc catatgcagc aggaagtaca atgcttggag tagttttacc tccttgggca
     2221 cattctggag gaactgataa gttaatagaa agattaagcg atgaaaacga aagagcaaaa
     2281 atgaaaaaag atatagcaaa tggaatagaa ggctgggata attttataga atttgctgga
     2341 atagaccaaa tatttgtaac aagcgtaaaa acagaaaaga ataaagatac aattggtaag
     2401 agcttattag aaattggaaa aatgagagga aaagacccat tagatgcaac gtttgattta
     2461 ttaaaagaag aagaaaatgc agttgggatg gtagatttct atggattaga agaacatata
     2521 ataggattca tgaaaagaga cgagcaaaat gtgtgtacag atggtctttt agcaggtaaa
     2581 ccacatccaa gagcttatgg ttctttccct aaaatactag gaagatatgt tagagagttg
     2641 aatgtactaa caattgaaga agctgtttat aaaatgacta aaaaggcagc tacaagcttt
     2701 agtataaaag atagaggcga attaaaagaa ggatattttg cagatatagt tatatttgac
     2761 aaggatacag ttagtggttg tgatgatttt atcaactcta tgcagtaccc tacaggtata
     2821 gattacgtaa ttataaatgg aaattgtgtt gttgaagaag gaaaatacaa taaaattaaa
     2881 gcaggaaaag tacttaaaaa ttaaatctta gctttaagga ggaataaatt tatgatttta
     2941 gtggggaatg ggaggctaat aagccaagat tctttaaatc cctatatgga agatggatgt
     3001 gtagtaataa atgataatct aattgaagat ataggtacta cagaagaaat gaaagctaaa
     3061 tatccagtac atgaatttat agatgcaggc aagaaaataa tcatgccggg tctaataaat
     3121 tcacatatgc atatatacag ctcatttgct agaggaatgg cagttccagg aaaaccttct
     3181 gagaactttt tagagatact aaataattta tggtggagac ttgacaaaca gcttacactg
     3241 gaagatacaa aatacagtgc atatgcaacg tatatagaat gcataaaaaa tggtgtaaca
     3301 acagtttatg accatcatgc aagtccaaat gcaattgaag gcagtttgtt tacaatatca
     3361 gatgttgcaa aggatttggg aattagaacg tgtctttgtt atgaagtgtc tgatagagat
     3421 ggtggaaata caatccaaca aggtatcaat gaaaacgtaa actttataaa atacgctcaa
     3481 aaagatgata cagacatgat aaaaggaatg tttgggcttc atgctgcatt tacactatct
     3541 gatgatacat taaataaatg tgcttcagag atggctggtc ttgatacagg ttaccatgta
     3601 catgttgctg aaggactagc agacttacaa tataatgtag ataaatatgg aaaaagggtt
     3661 ttagaaagat tcaatgattt tggagtacta aaagaaaaaa caatagcagt tcattgtata
     3721 tatataagtg cagaagaaat cagtctttta aataaaacaa atacatttgt agttcataat
     3781 ccagaatcta atatgggaaa tgcagttgga tgttctcctg caatagagtt attaaaaagg
     3841 ggagttacag taggattagg aactgatgga tatactagcg atatgtttga atcattaaaa
     3901 gttgccaata taattcacaa acatcactta aaagacccaa gagttggatt tgaagaatca
     3961 caaacaatgt tatttgataa caatagaaaa atagctaaag cttattataa aaatgaccta
     4021 ggagtactta aaaaaggtgc ttatgcagat gttatagtag ttgattatat accacatact
     4081 ccattgacag aaaatacaat agggggacac actatttttg gaatgagtgg aagaagcgta
     4141 gatacaacta ttataaatgg aaaagtactt atgaaagata gaaagttaat aaacatagat
     4201 gaagaaagta ttctagcaaa aagtagagaa ttaagtcaaa atctatggaa tagaatttaa
     4261 ataattgatt atgcaaaatt ctatgaagca ggaaccctgt gactttagtc atgggaggtt
     4321 cagattaata tacaagtaag aaacaagaat ttagatgtta aaaacagagc ttaaacaaaa
     4381 ataggaattt aaatgaagta tataaataaa aaatatacgg aatagaatat taaattaaat
     4441 atagaaggga gacccaaaat gtctcaagat aaaagcccta aaatagctcc agtagatgaa
     4501 aggttacctg tatccaaatc ttgggtattt tcactacagc acgttttagc aatgtgtgca
     4561 ggagcagtag cagttcctat gatgatagga agtgctgcac gactagatca tgctagtatt
     4621 gtttttttaa taaatgcttg cttgtttatg gcaggtatag gaacactcat acaaagtgtt
     4681 ggtataggaa atattgtagg tgctaaaata ccagtaatag aaggaactag ttttgccgct
     4741 gtggcaggta tgacagcaat agcctcatca tatagtgatt cacaagttgc aatacagaca
     4801 gtatttggct ctgtaatagt agctggattg ttttgtatat tacttgctcc agtatttgaa
     4861 aaattaataa gatttttccc tagtgtagtt actggaacag ttgttatggt aataggtatt
     4921 tcactatttc cagttgggat aaaatggata actgacaaca aattggaagc ggttgaacca
     4981 aactcattag cattagcttt agcagtattg gcaataactt tactttcatt taaattctta
     5041 aaaggaattt ggaatagcac agctatattg gtaggtatag tacttggtac actactttcc
     5101 atggcagttg gaatggcaga ttttagtgca gtaaatggag ttggttggct taacttaaat
     5161 gtacctttaa aatttggaat gcctagattt gaaccatctg caatactatc attgtgtttg
     5221 attatgttgg tacttatgac ggaatcagtt ggtaatatga tagctattca tgaaatggta
     5281 gacaaagaag taacaggtaa aaatattaaa agaggtctat ttggagatgg aatatctaca
     5341 gcattatctg gaatattcaa ttcatttcca ataactccat ttggtcaaaa tacaggaata
     5401 gtaggtctta caggtataaa atcaagattt atagcagttt acgcaggggt tatattgcta
     5461 gtacttggat tcttaccaaa atttgcagca ataataggag caatacctaa gcctgttctc
     5521 ggaggcgtag gatttgcaat gtttggaatg gtaatggttg gaggagtaaa aactttaagc
     5581 aaagtgaaat ttgatggaac taaaaatggg gttatagtag cagttagtgt tggtttgtct
     5641 atgataccac taacaaatcc cgatttctat cataatttcc caacttgggt acaaacaata
     5701 ttccatagtg gaataacaac aggtagttta tcagcagtgc ttttaaacat attcttcaat
     5761 gaactaggtt ctaaaaaaga aaaaaataat aaagttgaag aagcaaaata aaaataaaac
     5821 aagtaaaaat ctatattgac ttaaagtcat taagcattaa taaaaataat tcagagatat
     5881 taataattca acaaaactat cttatatttt aaataatata aaaaagtaaa taaataacga
     5941 atacaaataa aagaatacaa tctaaatatt aaaaatattt aatttaaggg agagataata
     6001 atatggctaa tttaaaagtt aatttatatg gaatggaatt tttaaaccca attatgacag
     6061 cggcaggacc tggagcaaaa gatggtgctc tctgtcaaga ggcagcaaaa ggtggagcag
     6121 gagctatatg tacaaaaact atatctgtat taccagctga tgtaccaaga ccatgtatgg
     6181 caaaaactaa tagtggtttt ttaaacacag aattatggtc agagcttcca aaagagcaat
     6241 ggatagagga agaatacaaa aaggtaaaag aaacagggct tccagtaata gtaagtatgg
     6301 gatattcagc agagcaaata gctgaggttg ctcctttagt aaagccttat gcagatgcag
     6361 ttgaattatc tactcattat gtaggtacag acgttgcacc aatagtaaat gctttaaaag
     6421 ctgcaaaagc tgttttagat gtaccagtat ttatgaaaat gagtcctcat actgacattc
     6481 aaactattgc aaaagctgtt gaagaagcag gtgcagatgg acttgtaatg ataaactcat
     6541 ttggaccatg tatggcaata gatgtaaata caggataccc tataatggga agtaaagctg
     6601 gatacggatg gttatcaggt gcaccaataa gacctctagc aataagatgt atatatgaag
     6661 cttctcaagt tgtaaatata ccaataatcg gtgttggtgg aatcacttgt ggaaaagacg
     6721 tggctgaaat gatgatggct ggagcaagtg ctgttcaagt atgtacagaa gcaatcctta
     6781 aagggccttc tatctatgga aaaattgcta aagaattaaa tgaattttta gatgagaatg
     6841 gatatgctga tgttaatgaa ataaaaggat taactcataa gaaaatggca gctagagggt
     6901 ttagaactca ttcagtagct ccagtagtgg atgataacaa gtgcatcaaa tgtaagcaat
     6961 gtgtaacaag ctgtgtatac gaagctttag aagttacaga taagttgaac atagatgcag
     7021 acaagtgttt tggatgtgga ttatgtgtaa ctagatgtcc aaaaggagct ttaagcatct
     7081 gctacaatca agtagaagct atgaatgagt aataattata tagaattttg gtaattaact
     7141 tagagtaata ggtattagga taataaaata tatcagataa acaaacacac attatgcatt
     7201 aataatacag tctaaagtta ataattttat tatattgatt taactaaata ttagtttagc
     7261 tgaatattaa tttaattagg tattggttta atcttaatat agatttacta cgtataagtg
     7321 aactaactag attactttaa gttaattttt tcatataaaa taaattgata cattacaagt
     7381 aaggtggagt ctatatggga attgttttaa aaggtgggaa agtagtaaca agtgaaaaaa
     7441 cttactgtgc agatgtaaga atagaagatg aaaaaatagt cgatataggt cttgatatat
     7501 caaaagaaaa tgatgaaata gttgatgtta gtgattctta tgttatacca ggaggtatag
     7561 atacacacac acactttgac ttagatacag gcgttactaa gacagcagat aactttgaga
     7621 caggaacaaa agctgctata gtaggaggaa ctactactat attggatttt gctacacaag
     7681 ctaaaggatg ttctttgaaa gacggattag aagaatggca tgaaaaagct aaggataaat
     7741 cctattcaga ttatggcttt catatggcta taacagattg gaatgactcg gtttgtaatg
     7801 aaatggagga tatggtgaaa gagggagtat cttcatttaa actgtatatg gcgtataaag
     7861 gaagccttca agttgatgat ggtgtaatct ttgaagcatt gagaaaagca gaagagatag
     7921 gagggattat tggtttccat tgtgaaaatg gggacataat atgtgaactt gtggataaag
     7981 caaagtcaga aaataattta tctccaaaat atcatcaatt aactagacca gctataatgg
     8041 aggcagaagc tacatcaaga cttatgaaga tagctgaagt tgcaaactca aaagtatatg
     8101 tagttcactt aagctgtaaa gaatccttag aggaggtttt aaaagctaag caaagaggtg
     8161 tggatgtaat agttgaaact tgtcctcaat accttctatt aaatgatgaa ttatatgatt
     8221 tagatggatt tgaaagtgct aagtatgtaa tgtcaccacc tttaagaaat aaagaaaata
     8281 atgaaatatt atggaatgct attttagata atcaaataga cactatagga acagaccatt
     8341 gttcttttaa ttataaggga cagaaggaac ttgggataaa cgatttcagt aagataccaa
     8401 atggttctcc aggtgtagaa cataggatga gtcttttata cacatatgga gtctgtaaaa
     8461 ataaaatatc tatcaacaag atggtagaag taacatcaac taatgcagca aagttgtttg
     8521 gaatgtatcc taataaagga actatagaaa taggcagtga tgcagatata ttagtcttaa
     8581 atccaaacaa aaaatccaaa atagaagcaa aaaaacaatt acaaaatgta gattatacac
     8641 cttatgaagg gtatgaagtg gattgtcaaa tagaacatgt ttatttaaga ggaaaagaag
     8701 tagttagaga tggagaatta atttgtaata ctccaagtgg gagatatata aataggcgtc
     8761 taacaaaggg ggctagagaa tggtagaatt tatattaaat ggagagaaag ttgttgcaaa
     8821 agaaggcaaa aaacttttag acttccttag agaagatatg aatataactt cagttaaaaa
     8881 tggctgttca gaaggtgctt gtggaacatg tatggttata atagatggta aagctactaa
     8941 agcatgtgta tttaaaactg ataaattagc tgggaaaaac ataacaacta tagagggatt
     9001 atcagagaga gaaagagatg tttttgcata ttcttttgct agtcaaggag ctgtacaatg
     9061 tggattttgt attccaggta tggtaattag ttcaaaagca cttatagatg ttaataataa
     9121 tcctactgaa gatgatataa agaaagcttt aaaaggtaat atatgtagat gtacagggta
     9181 tgtaaaaata attaaagctg tcgatttagc agcaaagatt tttagagaaa atatagatgt
     9241 gccaaaagta aactgtaagg gtctagtagg tgaaaatctt cctagagttg atgcagttgg
     9301 caaagcactt ggatattctg aatatgtaga tgatatgaga attgagggta tgatttatgg
     9361 ttcagcagtt agagctaagt atcctagagc tttagttaaa aaaatagata tagaagaagc
     9421 aaaagcttat ccaggagttg ttgcagttct aacagctaaa gatattccag gaactataaa
     9481 ggtagggcac ttaaagaagg attgggatac tttaatacca gaaggtgaaa taactagata
     9541 tttaggagat ggaatagttt tagtagcagc agaaactaga gaagcgttag aagaggccaa
     9601 aaagctagtt aagatagatt atgaggaatt aacaccatta agtactacag cagatgcact
     9661 tagagaagat gctccaaaga tacatgagac agggaatata cttgttaagg aacatttagt
     9721 aagaggaaac gctaaagaaa agatagaaaa ttcaaaatat gttgtaacta aaagatatac
     9781 tacacctgct acagaacacg catttttaga gcctgaatgt gcagtagctg gtccttatga
     9841 agatgatgga gtaataatct attcaacaga ccaaggggtg tttgccactc aacatgaatg
     9901 tgcagatatg ttagggttac catatgagaa ggtaagagtt ataaacaaaa tggtaggagg
     9961 agggtttggt ggaaaagaag acatgagtgt tcaacaccat gcagccttac ttgcatacca
    10021 tacaaagaga tttgtaaaag tacagttaac aagaaaagaa agtattattg ttcatccaaa
    10081 acgacatgcc acagaaatgg aatttacgac agcatgtgat gaaaatggat acttaacggg
    10141 tatgaaggca acgataatat ctgacacagg tgcatatgcc tctcttggag gaccagtgct
    10201 acaaagactg tgtactcatg cagcaggacc atacaattat caagatattg atataacagg
    10261 aacagcagta tatactaata accctcctgg aggagcattt agaggatttg gagttacaca
    10321 gagttgtttt gcaactgaat gtaatctaaa tttacttgct gaaatggttg gaatatctcc
    10381 ttgggagata agatataaaa atgcaataag acctgggcaa gttttaccaa atggtcaatt
    10441 tgcagatgaa ggaacagcaa tggcagagac actagaagct gtaaaagaag agtatgaaaa
    10501 gcatccacgt gcaggtatag cttgtgctat gaaaaatagt ggggttggag ttggtatacc
    10561 tgatgttgga agatgtagat tggtgataaa agatggcaaa gttcacataa gaacaagtgc
    10621 ttcttgtata ggtcaagggc ttggtactgt tatgactcaa gtagtttgtg aaactgctag
    10681 cctacttcct gaaaatatag tgcatgaagt tccagatact catttaacac cagactctgg
    10741 taatactacg gcttcaagac agacagtatt tacgggagaa gctactaagg ttgcatcaaa
    10801 taagttaaaa gaagctttag actctggcaa aactttactt gatttagagg gagaagagtt
    10861 ttatggagaa tatgaaagta ttacagataa aatgggttgt gacaaagaaa atccagtaag
    10921 tcatgttgct tatggatatg caacacaagt tgtaattctg gatgaaaatg gaaaactaga
    10981 aaaagtagta gcagcacatg atgttggaag agctataaat ccaatcagtt tagaaggtca
    11041 aatagaaggt ggagtagtta tgggtcttgg atatgctcta accgaagatt acccagtagt
    11101 taactctgtt cctacagcta agtttggtac attaggttta tttaaatcta cagatatacc
    11161 agaaatacaa gctttaatag ttgaaaagaa caaagattta ttggcttatg gggcaaaagg
    11221 tgtaggagaa atttgtacag ttcctactgc accagcagta caaaatgcct attatgtatt
    11281 tgataaagaa tttagaactg agctaccact acaaaatact ccatatagta aaaaaaagta
    11341 acaaaaaaaa gaaatattaa ataaaaaaat agatgaaatt gtaaataaaa aaaacccttt
    11401 tgcataaggt atattttaaa tgatagaata tacatataca ggagggtttt tcttttataa
    11461 aataagttta ataaaattga gaggtgagca gaatgaattt taataatagt ctagaattaa
    11521 ctcagtctca gaagctaata atgactacac aacttaagca atcattatca atattaaata
    11581 tgagtaagtt agagttagaa gaagagataa aaagagaagc agagaataac ccattagttg
    11641 aagtcgagaa aagtggggag ataaattggg aagagtatat aaaagacatg gataggtcaa
    11701 gacgtttaga tagaacagag ataagctata atcctgataa tgaagttaat ctagaaaatt
    11761 tagttaaata ttcgtcaaat ttatatgaag atttaaaatt tcagataagc ctgtataaac
    11821 ttacagacaa agaattggaa gtctgtgagt atataattga tagtttagat gaggatggat
    11881 atttaagaac tgaggaaaaa gtaattgttg ataccttaaa gatagatgaa gaactttttg
    11941 aaaaatgttt aattagtgtt cagcagttgg aacctagtgg agtaggggca agaagtcttt
    12001 cagaatgtct gatgatacaa atggctagtt taggaatata taatgaaatt ttagaggaaa
    12061 tcgttacaaa agacttaaat ttaattgcaa ataataaata taaagagata agtaaaaagt
    12121 acaatatgtc attacaaaaa tgtgttgaac taataaacat aattaagaca ttagacccca
    12181 aaccaggaag aacttgttca gtagagaaaa gtgtatacat tcaacctgat gtaacagtag
    12241 aaaagattga tgatgaattt attgtatatt taaatgagaa agattcttac caaataagaa
    12301 tcaatagtta ttataaagac atattaaaaa attcacagtc tgatgaaagt gcaaaagaat
    12361 ttataaaaga aagactaaat tcagcaacag gacttatgaa aaatatagag agtaggaaaa
    12421 ctacagtact taagattgca gaagagatag taaaagctca agatgaattt ttgagaaaag
    12481 ggacaaaata tataaaacct ttgaaaatga aagacatagc agaaaaatta gaatttcatg
    12541 aatctactat aagtagaggt gtaaatggta aatatatgct aacacctttt ggcgtatatg
    12601 aatttaggta ctttttcagt agcgccattg agactgagaa taatgaaatg gcatctagta
    12661 caagcataaa aaaaattata aaggaaacaa taaaagacga aaataaaaag aaacctttaa
    12721 gtgatgacca tatatctaag ttattaaaag aaaaaggaat caatgttgca agaaggacag
    12781 ttgccaaata tcgtgaagaa ttaggaatcc catcttcaag taagagaaaa gaatactaat
    12841 aagtaaaaga caaatatcag tatggtaagg ggaaaaggct aattaagaga tgaatgtaga
    12901 ttgggctaaa aattttataa agtaaaataa tagaatatgg aaaagtatta aaatgctata
    12961 aggtattaaa aaactgggga acattaaaac attaagatag caaagaacat aaaaaactaa
    13021 agaagattaa aacattaaag aacattaggc tttgaggaat attagaactt taaagaatat
    13081 taaaatttat aaaatataaa aatactaaaa agaattgaaa aactggggaa cattaaaata
    13141 ttgtaaaatg ctaaaaaact agaaaacata attaaataag tgcttataaa gaatgattaa
    13201 attaaataag tgtttgtaaa gaataattag atattagaaa aagaagaata cctagataaa
    13261 taaactatta ttaaatttaa taatcacaaa aggaaaacac gtagggtata aataaaaatc
    13321 ttaactagat aaaagtaaac ttagttgtag ataaaaataa ctagaaataa aagtaactag
    13381 aaataaaatt atttatagta ataaatatta gaatgaaaat ataaaaaaat atatataaaa
    13441 agccttatat aaaaagaaaa aatactgtga tttgaaaatg gtggagaaat attttataaa
    13501 ataaaaaaat aaaaaagttt ttgaaaaact attgaaatct atacgcaaat cctataaaat
    13561 aaaaatatct tgtgggactt aaataaacac tagggacaaa aaaagtcccg aaaggaaagc
    13621 aataaggagt atgttatgaa gaacttatta gaaattcaaa agaaattgat tccacaggca
    13681 attgaactta tggaaagaag atactctatt ttaagacaaa tatctttaag tgaaccaata
    13741 ggtaggagaa cattatcaaa tgtattagag attagtgaaa gaataatcag gtcagaaact
    13801 gagtttttaa aagaacaagg acttatagac gtagctgtat ctgggatgac tatcactaaa
    13861 gaaggttctg atttgttaga taaacttaag gacatcatga gtgacatcat gggattgtct
    13921 aagttacaag atagagtcag agaaaagctt ggaataaaaa gggtaatact agttcctgga
    13981 agctgtgatg aaaatggaag tttactaaaa gatgtaacta gatatggatg cgagtatttc
    14041 ttaggcattt taaaagatgg agatatcgtt tcaataacag gaggaagcac tatgttggag
    14101 ttttcaaacg ttattaaaac tgataagaga tatccaaact ctacaatagt tccagctaga
    14161 ggaagtatgg gaactgatgt agagatgcaa tctaacaata tagttgcttc aataagtaag
    14221 aaattacatt ctaattgcaa acttttacat ataccagatg agttggatga aagtgcaatg
    14281 aaaactctta gccaagtgcc agagattaag acaactttgg agtatattaa aagaacagac
    14341 attttagttt ttagtatagg tagagcagat gtcatggcaa agagaagaaa acttccagaa
    14401 gacaaggttg atgagatatt atctaaaaat gcagttggag aagcatttgg atattatttc
    14461 aacaaagatg gagaaatagt ttacaaactg aacactgttg gaattgattt agaaacagtt
    14521 aaacaagtaa aagaaactat tgcaatcttt gcagggactg aaaaagtaga agccttatta
    14581 gctatatcaa atatgcataa aaacatggtt cttgtaacag atgaagaaag tgcttataag
    14641 atattgtcat tattataaaa gtcaagacct agcacagagc tagaaaacat acatttactt
    14701 tataggaggt agcgaaaatg gttaaagtag ctataaatgg atttggaaga ataggaagat
    14761 tagcattaag aaaaatgatg gagcaacaag ataagttcga agttgttgca ataaacgact
    14821 taacagacgc taaaatgtta gctcatttat ttaaatatga tacagctcaa ggaagattta
    14881 acggagaaat agaagttaaa gaaggtgctt ttgtagtaaa cggaaaagaa ataaaagtaa
    14941 cagcagaaag aaatcctgct gacctaccat gggctgaatt aggagtagat atagtattag
    15001 aatgtacagg attctttaca tctaaagata aagctgaagc tcatatacaa gcaggggcta
    15061 agaaagttgt tatatctgca ccagctactg gagatttaaa aacaatagta ttcaacacaa
    15121 acagtgacat attagatggt tctgaaacag ttatatcagg agcttcttgt actactaact
    15181 gtttagctcc aatggctaaa gtattaaatg ataaatacgg tatagaaaaa ggattaatga
    15241 ctactataca tgcatacact aatgaccaaa atactttaga tggtcctcat ccaaaaggtg
    15301 atttaagaag agctagagct gctgctggaa acatagttcc aaacactaca ggagctgcaa
    15361 aagcaatagg cttagtaata ccttcattaa aaggaaaatt agatggggca gcacaaagag
    15421 taccagttgt aactggttca ataactgaat tagtttgtac attaggaaaa aatgttacag
    15481 ttgaagaaat aaatgcagct atgaaagaag cttcaaacga atctttcgga tatactgaag
    15541 aaatgttagt atcttctgac ataatcggaa taagctacgg ttcattattt gacgctactc
    15601 aaacaaaagt tatggaagta gatggaaaac aattagttaa agttgtttct tggtatgata
    15661 atgaaatgtc ttatacttct caattaataa gaactttagg atactttgca caattagcaa
    15721 aatagtcagt tttgagaaaa tagaaaagta gattgacaaa gtccgagttt gtagtttaat
    15781 actacggact caggactttt tttaagaaaa aatatataaa atacacctaa ataatgttac
    15841 atactatata taaaaatatg ataatatgat atatttaata gggtaaaagt atgtactata
    15901 ttatacttgg caataaaaaa ttactttatt aaaaggagag atatttatgt caatgcttaa
    15961 caaaaaaact atagaagata ttgatgtgtg cggaaaaaaa gtattagtaa gatgtgactt
    16021 taatgtgcca ttacaagatg gtgtaattac tgatgaaaat agattgaacg gagctttacc
    16081 aactattcaa tatttaattt ctaaaggggc aaaagttata ttatgctctc acttaggaaa
    16141 gccaaaagga gaagctaaac cagaattatc tttagcacca gtagcaaaga gattatctga
    16201 gatgttagga aaagaagttg tatttgcagc agacgataat gttgtaggag aaaatgcaaa
    16261 gaaagcaact gaaaaaatgg aaaacggaga tgtagtatta ttagaaaata ctagatatag
    16321 aaaagaagaa actaaaaacg aagagaattt ctctaaagaa ctagcatcac ttgctgaaat
    16381 atttgtaaat gatgcatttg gaactgctca tagagcacat tgctcaacag ttggtgcagg
    16441 cgagttttta caagagagag tttgtggata cttaattcaa aaagaattaa aattcttagg
    16501 agaagctgtt gcaaaccctg taagaccatt tacagctata cttggaggag ctaaagtttc
    16561 tgataaatta gctgttataa acgaattatt agaaaaagtt gataatctaa taataggtgg
    16621 aggaatggct tatacattct taaaagctca gggttatgaa gtaggaactt ctttacttga
    16681 aatagataaa gttgaatatg ctaaagaaat gatggaaaaa gctaataata agggtgttaa
    16741 cttattatta ccagtagacg ttgttatggc agaccacttt gcaccagatg ctactccaat
    16801 agtaacagaa gatgctaatg taaaagaaga ttacatggga ctagatatgg gacctaagac
    16861 tatagcaaac tttgtaaaaa ctataaaaga atcaaaaact gtagtatgga atgggccaat
    16921 gggagtattt gaatttgaaa actttgcaaa tggtacatta tcagtagcta gagctatggc
    16981 agagttgact gatgcaacta ctgtaattgg cggtggagat agtgcagcag cagttaatca
    17041 attaggattt ggtgacaaga tgactcacgt atcaacaggt ggaggagcat cactagaatt
    17101 tttagaaggt aaagaattac caggtattgc agcattagat aataaataaa ctaatatata
    17161 gaggagattg taaaatgaga aaacctataa ttgcaggaaa ctggaaaatg cataaaacaa
    17221 taaaagaagc tttagaattt gtaaatgaag ttaaagataa agtaaattct gataaagtag
    17281 aagctgttat atgtgcacca tttactttat taaaggattt aaaagaagct actaagggta
    17341 caaatataaa gataggtgct caaaatatgc actttgaaga aaaaggagca tttacaggag
    17401 aagtttcacc tcttatgtta aaagaaatag atatggacta tgttgtaata ggacattcag
    17461 aaagaagaca atacttcaat gaaactgatg aaactgtaaa caagaaagtt ttaaaagcat
    17521 tagaagtagg aatagaccca atattatgtg ttggagaaac tttagaacaa agagaagctg
    17581 gaaaaactaa agatgtttgt aaagttcaag ttgaaaaagc tttagaaaat gttcttaaag
    17641 atgatttatc aaaagtagtt gttgcatatg agccaatctg ggctattgga actggtaaaa
    17701 ctgcaactgc tgaagatgct aatgacgtta tatcttatat aagagaagtt ataaaaggat
    17761 tatatggaga attagctaat gaagttagaa tacaatacgg tggaagtgtt aaaccttcaa
    17821 atgtagccga aataatgggt caaagtgata tagatggagc tttagtaggt ggagctagtt
    17881 tagctagtaa tgattaccta gaccttgtta atttctaagg agtgaaatga tgaaaaaacc
    17941 agttgcttta attattatgg acgggtttgg atacaataag gatgttaagg gaaatgcaat
    18001 tgctgagtcc aaaactccta atttagatag aataaaaaaa gagtatccaa acacattgat
    18061 aaatgctagt ggtcttgatg ttggtctacc tgatggtcaa atgggaaatt cagaagttgg
    18121 acacactaac ataggtgcag gtagaatagt atatcaagat ttaactagaa taacaaaatc
    18181 tataaaagat ggtgactttt ttgctaataa agttttatgt gaagcaatgg ataatgcaaa
    18241 agaaaattct cttcatgtaa tgggactttt gtctgatgga ggagtacatt cacatataga
    18301 ccatcttaag gctataatca aaatggcaaa agataaaggc gtacaaaagg tgtatgttca
    18361 cgcatttact gatggtagag atactgaccc tcaaagtgca ttagaatatg ccaaagaagt
    18421 acaagctagt atggatgaaa ttggagtagg tgaatttgct acagtttctg gtagatacta
    18481 cgctatggat agagataaga gatgggaaag agtagaactt gcatacaatg ccatggttag
    18541 aggtattggt gaaaaagcaa attctattga ggaagctatt caaaactctt atgatgatgg
    18601 aaaaaatgat gagtttataa tgccaacagt tattatgaaa gatgataaac cagttggaag
    18661 tataaaggaa aatgattcaa ttatattctt caactttaga ccagatagag ctagacaaat
    18721 aactagagct ttagtttgtg aagagtttga tggatttaaa agagaagata ttaaaaactt
    18781 ctttgtatgc ttaacagagt acgatataac tatagagaat gtgcatatag catttggacc
    18841 tcaatcttta gccaacactt taggtgagta tctagctaaa aatggaaaaa ctcaacttag
    18901 agctgctgaa actgaaaagt atgcacatgt aactttcttc ttcaatggtg gagtagaaga
    18961 gccaaataaa ggtgaagaaa gactattgat accttctcca aaggttgcaa cttatgattt
    19021 aaaaccagaa atgtcagcat atgaattgac agataaagct ttagataaat taggagaaga
    19081 taagtttgac tttatagtat taaactttgc aaatcctgat atggtaggtc acacaggaag
    19141 tatagaagcg gctataaagg ctgttgaaac tgtagatact tgtgtaggaa aattgataga
    19201 taaaatagta gagttaggtg gaagtgcaat aataactgct gaccacggaa atgctgaata
    19261 tatgttagac ccggaaacag gaaaaactgt aacggctcac tctattaatc cagtaccatt
    19321 tattgtagtt ggtcaagaat atgaaagtgc aaaattattg gatggaggaa gattatctga
    19381 tatagcaccg actgttttag atatgatgaa gttagaaaag ccagaagaaa tgactggtca
    19441 ttcacttatt tcaaaatagt cgattctgat aagtagagaa taatatataa actacaatat
    19501 aaatttaaaa ataatgcagt ttaggtttat tttgtaaagt ttagacttta tgacataaga
    19561 tttagatttt gtaagttctg gatattgtaa ggtgtagatg aaggtatatt ttataaaatt
    19621 tacaagatag acaatttata aaataaaaaa ttataatata gataataatg taaataatga
    19681 tataaataat aacaagatag acaataacaa tataaataat aataattaat atatattata
    19741 taagcaaaaa ttttagaatc attagttgta cattttatgt ttaatgtttt aaatctagtt
    19801 ataatatttg tgtatttaag attggttttg gattttagac aagttttttt atattatata
    19861 aattaaaata ttataaaaaa taaaataaaa ataggaactt ttacaaattt acccatcaaa
    19921 taaatttgta cctaattttc aaaagcaact ttatgtacta taatatagaa agtagattaa
    19981 aatagtagac ggatgtttac agtaaatgaa aatttaacta atagaaaatc gaagggagat
    20041 atacaaatgt cagttattga attagtatat gctagagaag tattagactc aagaggaaat
    20101 ccaactgtag aagttgaagt agtattagaa gatggagcaa tgggaagagc aatagttcca
    20161 tcaggagcat ctacaggagc ttttgaagct gttgaattaa gagatggaga taagggaaga
    20221 tacttaggaa aaggtgtaga aacagctgtt gctaacgtaa atgaaataat agcacctgaa
    20281 atagaaggaa tggatgcatt tgaccaacca gctatagatg caatcatgat agaattagat
    20341 ggaactccaa acaaaggtaa attaggagct aatgctatat taggtgtttc aatggcagta
    20401 gctagagcag ctgctgatga aataggttta ccattattcc aatatttagg tggagtaaat
    20461 gctaaacaat tacctgttcc aatgatgaac atattaaacg gtggagagca tgctgataat
    20521 aacgttgatg ttcaagagtt catgatatta ccagtaggag cttgttgctt taaagaaggt
    20581 ttaagaatgg gagcagaagt attccattca ttaaagaaag ttttaggaga aaaaggatta
    20641 gcttgtggtg ttggtgacga aggtggattt gctccaaact taggttcaaa cagagaagct
    20701 ttagaattaa tagttgaagc tataacaaaa gctggatata agccaggtga agatgttatg
    20761 ttaggacttg acgttgctgc tacagaaatg tacaacaaag aaactaaaaa atacgtttta
    20821 gcaggagaag gaaaagagtt aactgctgct gaaatggttg ctttatatga agattggtca
    20881 aacaacttcc caataataac tatagaagat ggtttagacg aagaagattg ggacggatgg
    20941 aagttattaa ctgaaagatt aggaaataaa ttacaattag ttggtgacga cttatttgtt
    21001 actaacacag agagattaga aaaaggaatt gaaaatggag tagctaactc tatattagtt
    21061 aaagttaatc aaataggtac aataactgaa actttagatg ctatagaaat ggctaagaga
    21121 gcaggatata ctgctgttat atctcacaga tcaggagaaa ctgaagattc tactatagca
    21181 gatttagctg ttgcagtaaa tgctggtcaa ataaaaactg gtgctccatc aagaactgat
    21241 agagttgcta agtacaacca attattaaga atagaagaaa tggttggaga acaagctaga
    21301 tactgtggat taaaatcttt ctataattta aaaaaatagt tattgaagat taaaaatatg
    21361 gcattaaaga ttaaaaagat aacataaaag ataaaaattt atcttataag ggataagtga
    21421 tagttgattt ttcaattcac ttatcctttt tttatatttc atttcttact tttattcaca
    21481 gctttagaaa aaatacgaat aaaagtttct atttcatctt tatctaattg atgtaagaga
    21541 tttctaaata caattatttc tatgtctcta tgttcttgtt gagcctttaa acctaattca
    21601 gttaagacta agttatacct ccttttgtct ttaatacagg gattcttttt aataaatttt
    21661 tttaattcaa gacgttttac agcacttgtt aatgtgcttt ttggcaattc taaaatttca
    21721 ctaatttctt tcatcatact attgggatac tgttcaacta tttctaaaat acttaattct
    21781 actaaagaaa taccatgaat agtaggatac ttaccactac acatttctgt tttcatctgc
    21841 atactaacaa tatgccacag gttgtttagc tcttttacag gattcattga cttttacctc
    21901 catatatgat acaattatcg cactaatata gtacgataat cgtattaatt tgtcaatagg
    21961 atggtgtttt tatgaattta gagattatac gagctacaag tgaaaatgct atagaaatgg
    22021 gttttataca ttcttgttcg tggagaaaag cgtataaagg aattattcca gataagataa
    22081 tagatgaatt cacttctgag aaaagagcag aagtttttca aagtgttatt ccaaaagctg
    22141 aagaggaata ttatcttttt aaagtgaatg gtatacctgc tggttttgct tcattaaata
    22201 gaagtcaaga ggataatgct ccaaaatgtg ttggagaaat atattctata tatttccatc
    22261 ccgatttttg gggaacatct gtgacaaaaa aaggattaca attttgcatt gatagattaa
    22321 gcaatttagg atattcatat ataacgatat gggttctaaa agacaatact agagctataa
    22381 atttctataa aaaaaatggt tttacttgtg atgattttga gaaagagata catattggaa
    22441 aaaaattatt agaagtacgt tattcaaaaa agatatagta attgcatatt atttttgttt
    22501 gttatttata cttcaatagt cttgaaatga ctatattatt gtgatacact aaagagtaat
    22561 gaaatttaag taataaatag ataggagggg caataatgag caacatttta atgggtgtgc
    22621 aagtagtgct agggatttta ctagtaatta gcataatgcc tcaagattca aaaaatgctt
    22681 taccttcaga atttggtgga gaaggaagtc aagcttactt taaaccaaaa ggaaaacaag
    22741 catttcttgc tagagttaca aagataacag cagtattatt ttttataaat gcattggcat
    22801 tacttatagt taagaagtag tgatattgtt gaataactgg ccattattta attagtgcat
    22861 ttgctagata tttaaaaatt aaatttagtt tttaataaaa gtacatcaac ataaaaagta
    22921 cgtaaaataa aagctggact aatgatttta tatgattagt ccagttttta ttttatagat
    22981 tgactatttg ttggcattta ttatttgtat ttatatattg tttatattta tatatttttt
    23041 tcaaactaaa aaatatatag caacactact ttcaccatgt ttaatgagat aatatacaca
    23101 aagttatatt atatagtaaa aatagtataa taatatgttt ttacgcaatt aaatcaattt
    23161 tatatatgtt tctcataata ccaatttgtg ttatcaaata tcattaaata agattattct
    23221 gctttaaaat aaatattatt aaaaatactg taattataga aaatccagaa gtaaatacta
    23281 caatttgtcc agctaattca ctgtcagaat ccatctgttg tgccattgta aatgaagaaa
    23341 ctgcagttgg agcagataac attatcataa gtgaagttaa ttctacattt ctaaatccaa
    23401 gaaaaatgct aattggtaaa aatatagaag gtacaagtat aagttttcca catacgccca
    23461 atataatggt ttttatatgc ccctttatag cagaaaaact aaaagatccg cctagaatta
    23521 taagtgataa aggagtagct attttagaaa tatcaccaat agttttgtgt aaaaatgtag
    23581 gtatattaat atttaaaatt agcattgtca atcctataaa agaggcgatt atgagtggat
    23641 ttgttaagac gcttttaatc atatgtctta tatttacttt gccacctcta aaaatttcaa
    23701 gaattacaac agataataca ttaaacatag gtacaataac tgcaattaat aaagatgtga
    23761 ctccaatact cttatcgcca aatagtgaaa ttgtcacagg aataccaaat ataacgaaat
    23821 tgctacgaaa tacaccttgt attaatgcac cccttgtctt attatctttt tcaattttag
    23881 gaataataaa gactaagatt aagaagattg aaaaaataat tataacagct acagccataa
    23941 gcttaaaatt aagagaacca tctaaatctg tattgtatat attaaaaaat aataataatg
    24001 gcaagaaaga cttgaagcag acattattca ttttatttaa agtatgagta tcaaatagac
    24061 ctaatttttt tagaacatat ccaagtgaca ttgtgagaaa caaaggaaga acaacattta
    24121 ttgataggat taaattttcc aaggtttact ctccgtgatt ctatgatttg ataattcaat
    24181 taaatttcca ttagtttttt tcaaataaat agatttcata ttaccaaaga cacctgtaca
    24241 ttctacaatt cctcgcttaa tttgaatctc attttgttct aagtattttt ttggctcatc
    24301 aatatttgtg tttattttaa aacataagtt atctctactt gcttttaaat ttttaacata
    24361 aggtaaaaat ttataacctt ttatgttaag atttatctta acatttctag ccttaagttt
    24421 atacttttcg ctcatttctt tagatgaaaa accaaatttt ttataaaaat tgatataatt
    24481 ttctatatta ttattagtaa tgaccacatg ctctattttt tttatcatgg caactcctta
    24541 agattttata taaattagtt aaatttatat aagttattaa attttatata gattgataat
    24601 at
//
LOCUS       DAGQWR010000056        24357 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.056, whole genome shotgun sequence.
ACCESSION   DAGQWR010000056 DAGQWR010000000
VERSION     DAGQWR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.056"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            135..1493
                     /locus_tag="MIW95_001908"
     CDS             135..1493
                     /locus_tag="MIW95_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY3151236.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTIMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            1766..2146
                     /locus_tag="MIW95_001909"
     CDS             1766..2146
                     /locus_tag="MIW95_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY3151237.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            2271..2471
                     /locus_tag="MIW95_001910"
     CDS             2271..2471
                     /locus_tag="MIW95_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151238.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            2667..3395
                     /locus_tag="MIW95_001911"
     CDS             2667..3395
                     /locus_tag="MIW95_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY3151239.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            3417..4334
                     /locus_tag="MIW95_001912"
     CDS             3417..4334
                     /locus_tag="MIW95_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBY3151240.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            4476..5660
                     /locus_tag="MIW95_001913"
     CDS             4476..5660
                     /locus_tag="MIW95_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBY3151241.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            5767..5955
                     /locus_tag="MIW95_001914"
     CDS             5767..5955
                     /locus_tag="MIW95_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBY3151242.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            6105..6755
                     /locus_tag="MIW95_001915"
     CDS             6105..6755
                     /locus_tag="MIW95_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBY3151243.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            6858..7796
                     /locus_tag="MIW95_001916"
     CDS             6858..7796
                     /locus_tag="MIW95_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBY3151244.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            7822..8673
                     /locus_tag="MIW95_001917"
     CDS             7822..8673
                     /locus_tag="MIW95_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY3151245.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(8958..9692)
                     /locus_tag="MIW95_001918"
     CDS             complement(8958..9692)
                     /locus_tag="MIW95_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HBY3151246.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            9818..10534
                     /locus_tag="MIW95_001919"
     CDS             9818..10534
                     /locus_tag="MIW95_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBY3151247.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            10668..11360
                     /locus_tag="MIW95_001920"
     CDS             10668..11360
                     /locus_tag="MIW95_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3151248.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            11350..12531
                     /locus_tag="MIW95_001921"
     CDS             11350..12531
                     /locus_tag="MIW95_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBY3151249.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKLLYFNSEI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKMEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            12813..13268
                     /locus_tag="MIW95_001922"
     CDS             12813..13268
                     /locus_tag="MIW95_001922"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBY3151250.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            13258..13983
                     /locus_tag="MIW95_001923"
     CDS             13258..13983
                     /locus_tag="MIW95_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBY3151251.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLNQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            13955..16336
                     /locus_tag="MIW95_001924"
     CDS             13955..16336
                     /locus_tag="MIW95_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBY3151252.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDNAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSCGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(16541..17770)
                     /locus_tag="MIW95_001925"
     CDS             complement(16541..17770)
                     /locus_tag="MIW95_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBY3151253.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            18370..19284
                     /locus_tag="MIW95_001926"
     CDS             18370..19284
                     /locus_tag="MIW95_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151254.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGVKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            19308..19943
                     /locus_tag="MIW95_001927"
     CDS             19308..19943
                     /locus_tag="MIW95_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBY3151255.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTAQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            20319..21035
                     /gene="pyrF"
                     /locus_tag="MIW95_001928"
     CDS             20319..21035
                     /gene="pyrF"
                     /locus_tag="MIW95_001928"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBY3151256.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGADFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            21061..22104
                     /gene="carA"
                     /locus_tag="MIW95_001929"
     CDS             21061..22104
                     /gene="carA"
                     /locus_tag="MIW95_001929"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBY3151257.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGEILTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIAPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            22157..>24357
                     /gene="carB"
                     /locus_tag="MIW95_001930"
     CDS             22157..>24357
                     /gene="carB"
                     /locus_tag="MIW95_001930"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBY3151258.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYL"
BASE COUNT         9815 a         2558 c         4393 g         7591 t
ORIGIN      
        1 aacaagattt atattatttg aaagcagtat attataatgc gaacaataca attctaaact
       61 ttgcattgtc aatttacagt tcgataaact aattaaaaat taaatttata taaagaggga
      121 aaagaggaat agggatgttt ataaggaaaa atgttataaa gttagcaatt cctataatga
      181 tagagcagac gtttgtcatg cttttaggag tatttaatac aataatggca ggtcatattg
      241 gggaagaagc tgtatctgca gttggaatgg ttgattcaat aaacaatata tttatatctg
      301 tatttgctgc attatctgtt ggagctactg tcgttgtagc tcaacaaata ggaaaaaaag
      361 atagtgaaaa ggtgaatgaa actattaagc aggctctagt gtctggagta atattatcct
      421 ttacaataac aatattgatg tgggttttta gaactggtgt aattaattta ttttatggtt
      481 cagcagaaga attggtaaaa tcaaatgcaa aattatacat agagtttaca ttatttacgt
      541 atccatttat tgcgacacaa caaatagcaa atggtatttt aagagggtgt ggagatgtaa
      601 agaaacctat gtatgtgact atgtttatga atatagtaaa tgtaacatta ggatatatac
      661 ttatatatgg tattgattta aatcctctag gaataaattt gcaaacacca tcctatggca
      721 taacaggtgc agctatatct attgctatag ctagaataat tggatgtata gtaattataa
      781 gagctttatt taaaggaaat gaatttatat ctatgggtaa aatatttcca tttaagattg
      841 atgttgaaac tcaaaagtct atatttaata tagggatacc agctggagtt gaacaactac
      901 tttttaatgc aggtaaattg attgttcaag tatttatagt tactatggga acatcagcaa
      961 ttgcatccaa tgcaatagga atgtcaattg catccataat aaatgtaatt gggaatgctt
     1021 tagctttgtc tgctacaact ttggtgggac agtatatagg acgtggagat attaaaggag
     1081 ctaagagtac tttattatat ttaactaaat ttgcaacaat ttgtttattt atagtaggag
     1141 ctatatttat accaattgca ccctggattt caagtatgta tactcaaaat gctagtgtaa
     1201 ttgacatctc aacacaatta attcgaagtg attgtttagc tatggtaata tggcctatat
     1261 cctttgtatt atcagcaggg ctaaagggag ccggtgatac tagatataca atgatgactg
     1321 cagtaatagg tatgtggatt tttagaattt ttacaggtta cgtattgggg gttgtattag
     1381 aatttggagt gttaggaatt tggataggta tgtatactga ttggcttgtt agagggactt
     1441 tatattgttt aagattaaga ggaacaaagt ggctaaaaca caaagttgca tagaatatat
     1501 gaaatcactt ttagatggat aactctattt agaagtgatt ttttatgtag ttattatcat
     1561 aattataagt atgatatata aggttattaa attacataaa taaaggctat aatagtatgg
     1621 atattataaa ttatatattg agcagaatta cataatttgg cgataaatat tagaaatata
     1681 tatctataga aaataatata tatgggtata ttattaaata taaaaaatat taggtattta
     1741 tttaataata aaggtgggta ttgttatgat aaataaaata ggaaaaataa ctctctatgt
     1801 aaataatcaa gaggatgcaa agttgttttg gacagaaaaa ttgaactttg tagtaactta
     1861 tgaaaaggaa atgggtccaa atatgaaatg ggtggaagta gcaccaagta aaaatgaatt
     1921 tacaacattt gtgttgtatg ataaaaatct aatgatgaag caaaacaaag agataaatgt
     1981 atctcatcca tcaataattt taagtacaag agatattaat ggaacttatg aacaaatggc
     2041 aaataacgag gttgaagttg gggaattaat ggagatgcca tatggaagga tgttttcttt
     2101 taaagaccaa gatggaaatg attatttagt cagagaggat aaataatttt acaaagtatt
     2161 aaatttactc aatatctgat ataattagag acaataatta ttataaacta gtctaggatt
     2221 agaattatat aattaaaaat taagtgtaaa ttttgagtag aggtgataaa atgtttaaga
     2281 aaatggcagt actaaaagat atagcaacta aaataggtcg taaaaaagcg tatgaactat
     2341 tagaaatggt tgaaggtaat gatgcctttg tagctgaggt aaagataaaa aagaatggaa
     2401 tagaatctaa aaaagaagaa attatgttaa aagataatca aaaaataata ttagagtata
     2461 tagaaggtta aattttaaat acatataagt tgatatacta aatttctgag atgagtttac
     2521 ataaaactca tcttttttac ttttcaataa ctttgtttta ttatttaata tacattttat
     2581 ctgcttagag ttgctgatat ataaaaatta ctgatataat aaaagaatgg atattaatgt
     2641 aatattttat agaaaagagg ggtattatgc ttgaaataaa aaatctttcc tttaatgttg
     2701 agtctaacaa tgaggaacta ggaataataa atgatgttag cctttcattt gagaggggaa
     2761 aacttattgt tatcacaggt ccaaatggtg gaggaaaatc tacaattgct aagcttatta
     2821 tgggaattga aaaagctacg agtggtcaga taattttaga tggagaagat ataacaaatc
     2881 ttagtattac agaaagagca aaaaaaggaa taggatacgc atttcaacaa ccaccaagaa
     2941 taaaaggtat gacagttgaa aatttactta cacttgctca tggaaaacca ttgtctactg
     3001 atgtatgttg ccaatacctt acagatgttg gattgtgttc aaaagactat ttaaatcgtg
     3061 aagttgataa ctctctttct ggaggggaaa tgaaaagaat tgagatagca actctttttg
     3121 ctagagattt aaaggtttct atatttgatg aacctgaagc agggatagac ctttggagct
     3181 ttggaaaact caatgaaagt tttaagaaaa tacatgaaga gtctaatcag actataatca
     3241 taatttctca ccaagaaaga atattagagt tagctgatga aataattgtt ctacaagatg
     3301 gttcagtaaa aagccatgga acaaaagagg ctatattacc tgaaatcatg tgtcaagtta
     3361 attcaagttg tgaattaatg aaagacatga attaattaga aaggaaggca aattaaatga
     3421 acaaaataga tttaaatctt ttagaaaaag ttgctgaact tcgtggaact ccattaggag
     3481 catacaatat acgtaaaaat gggcaaggag tagagagaca ttcaactgaa aatgtaacta
     3541 taattcctaa aactgataaa cctggaatag atattatagt aaagccaaat acaaaaggag
     3601 aaaatgtaca tataccagta atagtaacag aggctggtgt acaggatatg gtatataatg
     3661 attttgaaat tggagaaaat gcagatgttg ttataattgc tgggtgtggt atacataact
     3721 gtggttgtga tgatagtgaa cataatggta tacatagatt tttcttacgt aaaggagctc
     3781 gcctaaaata tgtagaaaaa cattatggtg aaggtgatgg agaaggaaag agaattttaa
     3841 atccagttac tattgtcaat atggatgagg attcatattg tgaaatggaa acaattcaaa
     3901 ttaagggtgt agattcaact aaaagagaaa tggatgcaaa cctaggtaaa ggagctaaaa
     3961 tagttgtatt tgagaaatta ttaactcatg gtaatcaagt tgcagaatct aatatgaagg
     4021 ttatcttaga gggtgaaaac tcttctgctc aagtagtttc tcgtacagtt gggcaagata
     4081 attctaagca aaagtttaat ccttgtgtta ttggggattc cctttgttct gcacatgttc
     4141 aatgtgattc aataattatg ggaaattctc aaattagtgc tatacctgaa attgctgcaa
     4201 atcatccaga tgctctttta gtacatgaag cagctattgg aaagattgca ggagaccaaa
     4261 tactaaagat gatgacatta ggtcttactg aagaagaagc cgagcaacaa atacttaatt
     4321 gcttcttaaa gtaaattaaa gattttattt aatttaatat aattaatata gtataatata
     4381 gtgaataagt ctaaaatatt tttattttta ggcttatttt ctttatgtaa gaaaagctat
     4441 tagagaaatt atattgtaat taggatgtgg aataaatgaa aaatttactt ataagaaatg
     4501 ggacaataat atctccaaca gatggtatca aatttgaaaa aagagatatc ttggttttac
     4561 atgggaaaat atatgctatt ggattgaacc ttaaagagaa aataagaaaa gaaaaattac
     4621 ttacaaataa agagttcgag ataataaatg ccgataatat gtatatttcc ccaggattta
     4681 tagatgttca cacccattgt tataagagaa aattacctac aggtatggat tctgattcaa
     4741 ttggaattga aaaaggttct acagttatat ttgatgcagg tacagctggt ccctcaaact
     4801 actatgattt taaaaaaaag tatatggatg agtgtaaaac agaagtatat tcttttttaa
     4861 atgttacaaa tgagggacta aatatattga atgaatcaac ttctttagat gttataaatt
     4921 ttgacaaggt tgagtcaatt gtagaaaaaa atatagaaaa gataaagggt ataaaagtaa
     4981 aagcgagtaa atttcaatct aatgaaagtg gtatagatat aattaagaaa tcaaagcaag
     5041 ttgctaaaaa attaaattta cccttagtgg tatatatagg agagtatcca tcatatatag
     5101 atgaagtaat gaatattttg ggaaaaggag atgtggttac accagtatat ggaaatagta
     5161 caaatggaat ccttaaagaa tctggcacat taagaaaatc agtaataaat gcaaagaata
     5221 gaggagtatt gttcgatgta tgtcatggag tggaccaatt tgattttaaa gtttttaaaa
     5281 aagcaataaa gcaaggttta gagcctgacc taataagtac tgatatgcat ataaaaaaca
     5341 tgaaagacgt aaactattct cttttaaatg ttataaataa aattatggaa cttgggatat
     5401 ccctagaaaa gtgtatagaa aaagtaactg aatacccaac aaagattttc gatttacatg
     5461 gaaataaagg cagaataaag attggaggag aaggtgattt tacaatattc acaatggagg
     5521 agtgtaatga tattataaaa gactacaata ataatgaact tgttttaaat aaaaaattaa
     5581 gattgcaata tactgtaaaa tcatggatga aaagcagtga agtttataga catggatacg
     5641 aaaatactat aataaactaa acaatagtga taaaataata aatatatgtt taaattggta
     5701 tatattggat aaatatctaa ggaaacaaca attaaaaaat aataatttta ggggaggtat
     5761 tttagtatga aagcatttgt tgataaggat gtatgtgtag cttgtggagc atgtatagga
     5821 acatgtccag acgtttttag tatgagcgat gaagggcatt cagtagcaat tgaaggagaa
     5881 ataccagcag atttacaaga tttatgtaaa gaagccgaag aaggatgtcc agtttcagct
     5941 ataacagtta aatagagctg tttaaattta tttttaaaat aaatatataa gatttaattg
     6001 ggtacttaag ctatagtatt agaaatttct attactatag ctttttaaaa tagataaact
     6061 taatatgtaa gatattactt tttacaaata tggaggactg aaatatgaaa aaaaattatg
     6121 aaatagtatt attcgattta gatggaacac ttacagaccc taaagaagga ataacaaagt
     6181 ctattcagta ttctttaaat agctttggca taaaagaaga tttggaaaac ttagaccaat
     6241 ttataggacc accattacat gatactttca aggagtatta taaatttgaa gataaaaaag
     6301 ctaaagaagc tgttgaaaag tatagagaat attttgctga taaagggata tttgaaaata
     6361 aaatttatga gaatatgaaa gagatattag aaatgctcta taaaaatggt aaaatattat
     6421 tagtagcaac ttctaagcca acagtttttg cagaaactat acttagatat tttgatatag
     6481 atagatattt taaatatata gcaggtagca atcttgatgg tacaagagtg aataaaaatg
     6541 aagttataca atatgtacta gacctatgta atgtaaaaga taaagataaa gttattatgg
     6601 ttggagatag aaagtatgac ataattggag ctaaaaaaat aggtatagat tctattggag
     6661 tattgtatgg gtatggttct tttgaagagc ttagtgaaag tgaacctact tatattgtcg
     6721 aaaatgtaga gtctataaaa gatattttgt tataaatatc ttttatagac aaaaatatta
     6781 caaaatggtg tcgcatattt aaaaatatga tattatagtt aaggaagtca tttaaaaaag
     6841 aggaggatgc acttaaaatg aaatctattt taattatggg tggaagcgac ttcataggtt
     6901 ctgcacttgc taagcgcttg attaaatgtg ggtatcaaat tgatatactt acaaatggta
     6961 aaaaggaaat agattataat ggttttaaag aacatctaat ttgtgataga aaagttcgaa
     7021 aagatatgga aaacattatc acaggaagaa aatatgatta catatatgat atgacagcat
     7081 atacaaaaga agatgtaagt aatctgattg attttataag catggataac ttaaaaaagt
     7141 atatagtatt gtcagcagga gcagtatata aagatagtgg aagaaatata aaagaagaaa
     7201 atgagaaagg tgaaaatgaa aactggggaa aatatgggtt aaataaaaaa gaggctgaag
     7261 attttattat aaatagccct ataccatata taattattag accaacttac atatatggtg
     7321 aaaataataa tttatataga gagtattatt tctttgaaaa aatagaaaaa aatgaaaaga
     7381 taccagtacc aaaaggaaaa caagttagta atcaatttat atatattggg gatttggtaa
     7441 aggtattgga aagcataatg aaaaatcctc atgtaagaga ggcctataat gtgacaaatc
     7501 cacagttaat ttcttgggat gaccttatat atacatgtgg agaaattatt ggcaaagaac
     7561 ctataataaa atatgtagat atggaaaaag tagagtttag agaaagaacg tatttcccat
     7621 ttagaaatat agactttaat cttgatataa ataagcttat tgaacatggg ctttatatac
     7681 caaatgtgtt actaaaagaa ggtcttacag caacatataa atggttttca gcaaataagc
     7741 caaaaatgca tgacagaaaa atgaacaaag ttgagtctgt aatgcaaata gtttaaaaaa
     7801 ataaataagt aggtagatat tttgaactca aaaattaagt attttttaaa attttttatc
     7861 ttaatattta ttttgatttt tatatactca gttgcgatag agccgagtct attaattgtg
     7921 aaaaaatata atataagtga aaatggagca ttgataaaaa atgatacaat tgagagtaaa
     7981 tctgaaagtg taaaagtagt tcaaatttca gatactcaaa taggtagttt ttattcaact
     8041 aagaatctca aaaaagtagc taataagatt aacactttga atccagatat aatagtattt
     8101 actggtgatt taattgatta ctccaataaa aatccaagtg tagatgaaat tacaactatt
     8161 ttatcaagta tgaatgctag gttgggaaaa ttttcagtat ttggcaatca tgattatatg
     8221 tataaacttc ctaggtatta taggcagatt atgaaaaatt ccaacttcaa tcttttagta
     8281 aatgaaaata aaaaaataaa attaaaagat gataaatata ttaatatatt aggtgttgat
     8341 gaaatattaa atggaaatcc taatataaaa tatctggaga gtcagataga taataaaaat
     8401 tttaatttat tgttagcaca tgaaccagat ttagtagata tgcttagtaa agatacaatg
     8461 aatttagttc tatctgggca tagtcatggt ggtcaaataa gattacctat taagggagca
     8521 ttagttactc ctccatatgg gaggaaatat actaaagggt tttatgatat aaatggaaat
     8581 catttatatg tgagttcggg tcttggaagt acaaagttac catttagatt ttttaatata
     8641 ccagagataa tagaatttaa tattataatc taacataaaa tatttagaaa atataatttt
     8701 gctttttata atatgtagat gtatatactg taaaaatgga ttaaaccagt agattgaatg
     8761 aattaatata ttaaattaat atataaaata tttaagttat aattaatgta ttagacaaaa
     8821 taaatactat taagaactat taaaatatag attaaagata ataacatata ataaataaaa
     8881 aagtaagatt gtgtagatta tataaagatt atttataatt aatacaatct tgctttttta
     8941 tttaaaaatt ttaattatta tttaaaattt tttatcttct ctttatccca tataaacaat
     9001 ttctcagaat tatttaaagc gccaaataca tttcttttaa aatcaggcat ctctttattt
     9061 aaattagcaa taagattttt tatgtactct ctcctagtat gaccatttgc agggcaggga
     9121 tttgtgataa taggatagtt agaatctttt gaaattttct tagtcatata ctcttctatg
     9181 taaatcatag gtcttataac tgttaaatcc tgtctatcta ggtatgtctt tggggaaaaa
     9241 caatttactc taccctcata aaacatagac attaaaaatg tctcaattgc atcatcttta
     9301 tgatgaccaa gagctacttt attacatcca agttttttag cattatcgtt caaagcacct
     9361 cttcttaaat tagcacataa tgcacaagga tttttttctt gtcttatatc aaatacaatt
     9421 tcttttatgt cagtttgaaa ttcataaaaa ggaacattta tttctttaca caatttatct
     9481 agtggagaat tatcaacacc tccagtattt aatgttatgg ctatcaattc aaagttttgt
     9541 ggagaaaatc tttgataact tttaagtata tgaagtagag ttaaactatc ttttccacca
     9601 gaaagtccaa ccgcaatttt atcattttct tgtatcatat caaaatcttg tatagcctta
     9661 cgagctttac ttagaagttt ttgtatagac attttatcac catcctttaa aaatattaaa
     9721 tttaagattt atttaagatt tacttataaa atacttaagt cttatactct aaaattaaca
     9781 ttatagacaa acataacaaa aaggagaaga tttaattatg aaaaaattta aattaatagg
     9841 agccattatt ttagtagtat ttataagtgt atttatatct aaagatagat tacttaagaa
     9901 agataaagat ccaatagtag ctgctcaaca tgattcagaa caagatgttg aggcaaattc
     9961 tcaaaataaa aatcactcaa atagtatatc aaatatatta ttggtaaaca agacaaatgg
    10021 aattagcaaa aactatactc ctgaaaatat tactaaggta aacatacctt ttgttgaaga
    10081 agcaacagag gaagaaaaac aaatggcagg agaacctgca aaggcagttg aagatttagt
    10141 taagcaagct aattcagaag gaatacagtt tttagggtct tcagcataca gatcttatga
    10201 tactcaactg gatacttata caagaagggt aaaatctcag ggacgagaaa aggcagatgc
    10261 atatgtagca aagcctggtt acagtgagca ccaaactgga ctttgtattg atttaacaaa
    10321 tccagaaaga tggtttgttg gaagtacaaa agaagctaaa tggttggcag aaaatgctca
    10381 caagtttgga tttataataa gatatcctga aggtaaagaa gatataacag gtacagcata
    10441 tgaaccatgg catattagat atgttggtaa ggatgctgcc gaggaaattt attcaaaagg
    10501 attaacttta gaagaatatt tacaaaataa ataatataaa tgtgtaaata atttgtatta
    10561 tattggaaat ttagaaatga ataaatttat agattacact aactaaaata tagtataatt
    10621 ttatatgaat aaagctatat acaaagatta agttaggatg tgtaaaaatg aataatacag
    10681 ttcttgttgt ggatgatgaa aaggaaataa gagaccttat agagatatat cttttaaata
    10741 gtggatataa tgtaattaaa gctgaagatg gaaaagaggc tctagatatt ttaaaaaagg
    10801 aaaatattca tttaatgata ttggatataa tgatgcctaa aatggatggg atagaagttt
    10861 gtagaaaagt tagagaaagt ttgaatatac caatacttat gttaagtgca aaatcagaag
    10921 atatggataa aatacaaggg ataatgactg gtgcagatga ttatttgaca aaaccattta
    10981 acccacttga acttactgtg agagtgaaat cactgattag aagagcatat tattttagtg
    11041 gtgctaataa tgaagataaa gatgtcataa aggttggagt agtaattata gataaggaaa
    11101 aacatagtgt aacagtggct ggaaatgaaa taattttgac atcaagagaa tttgatatat
    11161 tatatttact agcaaataat aaaggcagag tatatagtac agaagaaata tttgaaaagg
    11221 tatggaaaga gaaatattac caatctaata atactgtaat ggttcatatg agcagaataa
    11281 gggataaaat tgaaaaatat acagatggag aaaaaataat tcatactgta tggggagtag
    11341 gatataaaat tgaaaaataa aagtttatta aatcatatgt ttcgacccat attagatctt
    11401 ataataagct gtgctttaac ttcaatgctt attttaacaa gtatgtatat aataaaatta
    11461 ttatatttca atagtgagat atctgtaatt aatcaacttg tatatcagtt aagagatttc
    11521 agaagtctta taggagagag tttattttat ccatttttat ttacaatcac atcattagca
    11581 atttattcca tgatatcgta caagagaaat aagcaaattg tagattttat aagaaagact
    11641 aatagaaaaa atcaaaaatt agaaatcatt aaaagtgctg taaatttaat ggatgaaggt
    11701 aacttagaaa aaagtataga tattgagtat gatttagata tattaaagga taaagaaaag
    11761 gatgatattg atgaactagc acataaaatc aataatatag tccatcaact tagaaatata
    11821 actattgaag agagacaagc tcagcagaca aaaacagatt taataacaaa tgtgtcacat
    11881 gacttaagaa cacctttgac atccataatg ggttatttag gagtgataga agaaggtaag
    11941 tataaagatg aagttcagat gatgtattat gttgatattg catatgaaaa atcgaaaaat
    12001 ttaaatgttc ttataaatga tttgttcgaa ttgactaaaa tgcaaaataa tacaataaaa
    12061 ctaaacaaaa tagaaataaa tttagtagag ttattaagtc aagtagtatc tcaatttgaa
    12121 atttatttta aacaagaatt tatggttagt agaattaatt ttaatgaaga aaaattaatt
    12181 gtgaaagctg atccaaataa attggtaaga gcatttgaaa atcttataac taatgctgtg
    12241 aaatatggaa aagatgggca ttatgtagat atagtcacag agaaaatgga agatatggcg
    12301 gttgtaaaag tgataaatta tggagaacca attccagtgt tagatttacc aaatatattt
    12361 gatagatttt atagagttga aaaatcgaga aatagaaatg ttggaggttc tggattgggt
    12421 cttgcgatta caaaaaatat tgtaaattta catgatggag aaattactgt ttcaagcgat
    12481 aaatatcaaa ctgtgtttga ggtacaactg ccaataatac aatcagctta gttataaaat
    12541 aaaagtacga ttatgtgata taaatattat aacaataaag tgtttacaaa aataaaaaag
    12601 tgatatataa tttaagtcag tttaataaga tacttatcaa gaattagggg agagagttag
    12661 ctcagtgatc ctataccaac cttttatcag gtggtcctgc taacaacgat gagatgactt
    12721 gaaataatat actcttctta tcaataatag ataggaagag ttttttttag aattaatgta
    12781 acaaacttta aagtataatg gaggattaaa taatggaaaa agtagaaagt tttaagctag
    12841 accatacaaa agtaaaagct ccttttgtaa gaaagtgtag tgtacttgat ggagtaaagg
    12901 gagataaagt aactaaattt gacttgagat ttttacagcc aaatgtagaa agttttggaa
    12961 ctgctgctat gcacggttta gagcatcttt tagctactta tttaagagat acattagatg
    13021 gtgtaataga cttatctcca atgggatgta gaacaggatt ttatttaata ctatggggag
    13081 atgtagatgc caaaacagtt aagattggtt tagaagaagc attaaaaaaa gttcttgaat
    13141 cagataaaat gccagcagct acagccatag aatgtggaaa ctacagagac ttatctttat
    13201 ttggggcaaa ggaatatgca aaagatgttt tagataaagg attttcttta aatatatatg
    13261 gagaataatt taattggaag agaagatata agcataaatc aaagtgaagt tttaagatat
    13321 ttacaataca gaggtcaaga gatagataaa gaccttaaat atactattga tgaatgtgta
    13381 gctataacta aaaataaaat aaatcctaga tatttatcta ggatttatcc tattagaata
    13441 aataaagata atgttgtaga attagaggga acaaatttaa cattagagag taaagatgta
    13501 tatgaacttt taaaagaatg taatgagtgt gtaattatgg cagctactat agggatagat
    13561 atagaaaaag aaataagacg acactcttat tcaaatttaa caaaaggact aataattgat
    13621 tcatgtggaa caacagcaat agaagaagtg tgtgacattg ttcaaaataa tttagaaaag
    13681 gtttttttaa accaagataa atttataact atgcgatata gcccaggata tggagattta
    13741 ccaatagaaa aaaatataga tatattaaac gttttgaatg ctcaaaaaca aataggtcta
    13801 acaattactt ctaatggaat tatgataccg agaaagtcag tagtggctat tataggtatt
    13861 tctaatacaa gaataaataa atctaagaaa gaaaagtcgt gtgaaaattg taaaaactat
    13921 agaaactgta catatagaaa aggagcgaac tcttgtggaa ataagacaat atctgaaaaa
    13981 taatatatta atatttgatg gtgcaatggg aacaatgctt caacaaaaag ggttaaagtt
    14041 aggtgaaaat ccagaagtat ttggtcttca aaatccagat aaacttatag aaatacatac
    14101 agcatactta gaggcaggtt cgaatgtaat tcttacaaat acttttggat gtaatgaact
    14161 aaaattagat agtaagtata ctgtagaaga ggtgattgac aatgctgttt tggtggctag
    14221 aaaagctata gaaaatgtag ataatactaa accaagatat gtagctttag atattggtcc
    14281 tataggagaa atgctagagc ctatgggtac tttaagtttt gataatgcat atgagatatt
    14341 taaaagacaa gtactacaag gcgttaaatc tggagttgat gtaatagtaa tagagactat
    14401 gatggattta tatgaagcta aagtagctgt attagcagca aaagaaaact ctgatttacc
    14461 tattttctgt actatgacat ttgatgaagg tggaagaagt tttactggat gtatgccaga
    14521 atgtatggtg gctactatag aaggtctagg tgtagatgca ataggagtta attgttcatt
    14581 aggacctaaa caacttttac caatagttga aaaaatagca tcaagagcta cagtccctgt
    14641 tatggttcaa gcaaatgcag gtcttccaaa tatagttgat ggagaggcta tatatgatgt
    14701 tgatgcaaaa gaattttttg aaggggttaa aaagttcgtt gaagttggtg ctactataat
    14761 aggtggatgt tgtggtacga atccaagttt tataaaagaa atatctgaga atataaattc
    14821 tgttacaaag ggatgtatcg agaaaattga taaatgtgta gtctgttcac catcaaaatt
    14881 tgtggaagta caatcgccta cagttgttgg agaaagattg aatcccacag gtagaaaatc
    14941 tctacaagaa gcattaaaaa atgaaaatgt tgattatgct ataaatttag gcttagaaca
    15001 agtaaatgct ggagcacaaa tacttggagt aaatgttggt cttccagaga ttgatgaaaa
    15061 aaaactaatg ccaaaattaa taagagagat tcaagcagta gtagatacac ctttacaagt
    15121 tgattcttcc aatgtagagg ccttagagca aggacttaga tattataatg gtagaacaat
    15181 agtaaactct gtaaatggca aagaggagtc tttagaaagt atattaccaa tagttaaaaa
    15241 gtatggttca tgtgtggttg gacttacact tgatgaaaag gggataccta aaaaggctga
    15301 agaaagattt gaaatagcaa aaaggatagt caatagagca gtttcttgtg ggataaaacc
    15361 aaaagatata tttatagact gtttatcatt aactgtttca gcacaacaag aagaagcaat
    15421 agaaactata aaagctataa caatggtaaa gacactagga gtaaaaacta tattaggtgt
    15481 ttcaaatatt tcatttggtc ttccaaatag gaaagcactt aatgcttctt ttctaacatt
    15541 agctttaggg gcagggcttg atttagcaat tataaatcca aatgaatata gtatgatgga
    15601 agctatcaat tcttttaaaa tattaaataa cacagataaa ggatgtataa actatataaa
    15661 tcaatatagt aatataaata attcaaaaaa atcggattca tctacaaaga ttgacaaaga
    15721 tttgccattg gatattttag ttgaaagagg attaaaagat gaagcaaaaa atgtaacact
    15781 gaatctttta aaagagaagg atgaaaatta catattagat gagattttaa ttcctgctct
    15841 tgataaagta ggaaaaagat atgatagcgg agatatattt ttaccacaat tgattcaatc
    15901 agcagaaacg gtaaaagttt cattaaatac aataaaggaa actttattaa gtaaaagtag
    15961 caataatgta tccaaaggaa aaataatagt tgctacagta aaaggtgata tccatgatat
    16021 tgggaaaaat atagtaaaaa taatgctaga aaattatgga tatgaggtta tagatttagg
    16081 taaagatgtc cctattgaag aagtagtgga agttgctaaa aagagaaata taaaattagt
    16141 aggattaagt gcacttatga ctacgactgt tcaaagtatg aaagatacaa ttcaggcact
    16201 gagagataat gagataaatg caaaggtatt tgtaggaggt gcagttttga cagaagaata
    16261 cgctgaagaa atgggcgctg attattattc aaaggatgca aagtctgctg ttgaaatagc
    16321 aaagttaaat ttttagcata taaattaaaa taaaggtttt actaaatagg atattttata
    16381 tataatataa atataagttt tagatactta tgtttaaaac aataggaatt aatctataat
    16441 aatgagatta attaatataa aaaatattta tcatcaacat taaaaagggc tgggaagtct
    16501 ggtataactt ccattagccc tttttagtta aaatcatatg ttaaaaagcc caattaccat
    16561 ttttaaaaat ctgtatttgt tttccatcat aagtctcacc aataatatcc atatcagcag
    16621 aaccaatcat aaaatctaca tgtgttaaac tgtcatttac accatgttca tctaattctt
    16681 catcttttaa attgtttcca ccttccagac aagtcttata agcagaacca attgcaaagt
    16741 ggcaagaagc attttcatca tatagagtat tataaaaaat tatatccgta ttggatattg
    16801 gtgagttgta tggaacaaga gcaacttctc ctaaataatg agagccttca tccgtttcaa
    16861 taagtttttc tagtgtatct aatccctttt cagcagagaa atctattatt ttaccatctt
    16921 taaatgttaa gaagaaattg tcaattatgt ttccaccata aacaagaggt tttgtactat
    16981 aaacagtccc atttacttta aacttatgtg gcattccaaa aatctcttca gttggaatat
    17041 tcggattaaa acttattcca ttagggtctt tggatgctcc acttagccaa acatgaccct
    17101 ttggcaagtc tagagtcaaa tcagtttttg aggatttata ttttaaagtt ttaaagttat
    17161 ttttatttag aaagtccatt ttagatttaa gattttcatt atgttcttcc catgctttta
    17221 tagggtcctg accatctact cttgaacatt taaatatagc atcccacagt ttttctattg
    17281 ctttatcttc tgataaattt ggaaacactt tttttgccca atcttttgtt ggcacagaga
    17341 ctatactcca tttacattta tcagttagag tgtaagaacg ccattctttt aaagcttttc
    17401 cagatgcctt ctgaaaattt gcaactcttt cagggtcaac atcttttaat aaatctggat
    17461 tttgggcata gactgataaa aatgccccac cttcttttgc tatatctaca tattgttctg
    17521 ctgtccattt ggggaactca tgaaatgctt catctggagc atacaagtat ttaataagag
    17581 tactttcttc atcagaccac tctatataaa catttttagc accacattta tatgcttctt
    17641 taagtgcatt tcttgcaaaa tcagcacatt ctattggtga ctttataagt agaatttgac
    17701 ctggctgtat gttaactcca acttttattg ctaattcagc gtatttttct aaatttctat
    17761 ttaaattcat atttatcctc ctatattttt aatattgaat aacttataaa gtattataac
    17821 attacttatg tttttaatat tgaacaactt ataaaatatt ataactttac ttagattttg
    17881 ggtcatattc aaaatattta tactttatag attgagaaat aaaaaaaata ttttaattaa
    17941 aacagattat gaggataaca aacttattta agcagtatgt tttttgttga taataatatt
    18001 aaagtagaaa taaaaatact tttttagtga aaagctatac aagaataccg aaaaaaactt
    18061 ataatataat aatgagatag atatttataa atgaaaaaaa tttttctggc aaagcttaat
    18121 taaaaaggta atagctagtg ttgttattta aattagcata gaaaatcaaa taaaaaaatt
    18181 atgctaatgt atagacattg ataaggtaga atttaagcct tttgaatgct atattaaact
    18241 agagtagata ttcttatgaa tttcaaaata gggcaggagg aaaaaggaaa taaaactata
    18301 aaaaatataa agtatttata taaatttata gagtttatac gtcagaagat ttcagttagg
    18361 agggcataaa tggagcttta tgaagccatt ttttatagaa aatctgtaag aaatttttcc
    18421 agtaagaaga taaaaaagcc attgatggaa gaaataaaaa gaagttgtaa aaatttagaa
    18481 tacttaaatg aagatttaaa tattaaagca catgttgttg atagaggtca tataatacat
    18541 tttttgatgg gtaaggaatg taaagtaaag gcacctcatt atatagtagt tacatcagac
    18601 aaaggtgagg actatttaca gaatatagga tttgctacag aaggtgtagt tttgcgcctt
    18661 acaacattgg gaatagctac ttgctggttg gaaggaaact taaagagaga agatatcttg
    18721 gaatttgtag atattgggaa taagaatttt gaagaagatg aggaagatga tttagattat
    18781 ctagctggag taaaaacaga agaagatgat gaaaacaaaa tagaaaatcc ttatataata
    18841 atagcatttg gatatccagc aaaaaatgaa gagttgttta ggacgagata tgctagacct
    18901 gatagaaagc cagtaaaaga tatgataaat aagataaata ggaatagaaa gtggaaagat
    18961 atcttagaat tgacaagaag agctccatct gtaaaaaatt ctcaaccgtg gtatttccat
    19021 aaggatgaac gaggtcttca tttatttgaa aagagaccta agaaacactg tgaagatatg
    19081 aataaggtta gcttaggtgt agccttaaga cattttgata tagcttgtat aaaaaataaa
    19141 atagatgttt catatgaaaa acttcctata aggaataaaa taggaaaatc atattttata
    19201 actgtagtcg agcatgtgaa accagaagaa gaaacccaag aagaaaatgt aactctagaa
    19261 aaagaagaat cacaagatga ataaaatttt ggaaggggat attggatatg tataagtgtg
    19321 gaaaatgtaa tcttagaggc tgttctaatg gaaatatgga taaattaatg aagatatgtc
    19381 caactagaga aattgaacta caacaaaaag caaagcaact ttatgaagaa gaagagaata
    19441 gaaaaatagc ttataatgct ggattagttg aagcagaggg gtattgtcaa tatactagac
    19501 taaaagagat tatagagttt gctaaaaagt gtggttataa aaagattggc atagcatttt
    19561 gtattggact aaaaagagaa atgcaaacag cacaaaaaat atttgaacat catgggtttg
    19621 aagtattttc tgttatatgt aaaaatgggg caatgcctaa gagtacaatt ggactagaag
    19681 catctcaaac aatatcaaag tgttcagatg aggtaatgtg caatccaata ggacaagctt
    19741 tatttttaaa tgaagctaag gttgacctta atgtaattct tggattatgt gttggtcacg
    19801 atactttagc catgaaatat atggaagctc ctataacaac tttagttgtc aaagatagag
    19861 taactgggca taatccagtt gctgcaatat ataatgcaga atcatatttt gagaaaaaat
    19921 tgtttgataa agatgaagaa taattcaaag acttattgac ataaaaagca attagtggtt
    19981 taattataaa aaagataaaa ctttaaatta tcctatagat acataaagtt atataaataa
    20041 agaaaaatat tacagctaaa ttccttaaag tagtccagag agactaaagg gttatagata
    20101 atgttagtat ataacctcta gtaaatcttc tagaggtttt tgatgagtat aaaataaaaa
    20161 tttaaatata ataaagtctt ttaaactagt ccagagagac tgggaaagat atctcatagc
    20221 taatgaatgg agttatgtat agataaaatc cttatgcctc ttttggtgtg aggatttttt
    20281 tatgattatt taggctttat taaaaaggag atgtaaaaat gataaatggt aaagaaaaat
    20341 tgatagttgc aattgatact gacgaatttg ataaagctaa agaactaata gacagattag
    20401 aagatagtgt agacatattt aaggtaggtc tagagcaata cgttgccaca aaaggtaaaa
    20461 caatagatta tcttaaagaa aaagggaaaa aaatattttt agaccttaaa ttccatgata
    20521 taccaaatac tatgaaaagt gcagtgagag ctgcagtgag agataatgtt tggttaatga
    20581 caatacatgt atcagatatg gaaggaatga gacaatgtgc actaattgct agggaagaag
    20641 cagaaagatt aaatatagaa aagccattag tagttggagt aacagtttta acttcattaa
    20701 gtaaccaaga tttacaggac ataggatgta atatgacaac agaagagtta gctataaaga
    20761 gagctaaact ggcaaaagaa tcaggcatag atggggttgt ttgttcagcg caagaagtag
    20821 ataaaatagt agaagcatgt ggagctgatt ttataacagt ttgcccagga ataagaccta
    20881 aatcagcaga ggttggagac caaaaaagag ttgtaactcc atcagatgca ataaaaaaag
    20941 gtgctcatta tttagttgtt ggaagaccta taacaaaagc agaaaatcca agagaatcag
    21001 ctattaatat tgtaagagaa atagaaaatg cttagataaa atatttggga ggtagcttat
    21061 atgaagggga aattaatttt agaagatgga acagtatttg aaggaaaagc atttggatat
    21121 ctaaaggaag ctattggaga agttgtattt aatacttcaa tggctgggta tggagaaata
    21181 ttgacagacg caacttatta tggacaaata gttactatga cttatccact tataggaaac
    21241 tatggaataa atttagaaga tgttgaatca aataaggtgc aagtaaaagg acttattgta
    21301 agagaaaaaa gtgataaccc aaataatttt agatgtgaaa tggatataga cacatatcta
    21361 aagcaaaata aggtaatagg acttgaagga atagatacaa gagcattaac taaaattatt
    21421 agaaacaatg gaactaagag aggaattata actttagaaa atgtatcatt acaagatgtt
    21481 cagtctaaat tagaagagca ttgtaacaaa gattcagtaa aaaatgttac aagaaaagaa
    21541 attgaaaata taaaaggtac tggagctaaa gttgtagtta tagactttgg tgttaaaaat
    21601 agtgtactag agtctcttaa agcttgtaac tgtgatataa ctatagcacc agcaatgact
    21661 acagtagaag aaattttaaa tataaatcct gacttaatct tattatctaa tggaccagga
    21721 aatccagagg atataaatga tatagttgaa aatataaaaa aattaatagg caaaaagcca
    21781 ttgacaggaa taggtttagg acatcaaatt ttagcattgg cattaggagg aaagacttca
    21841 aagcttgtat ttggacatag aggaggtaat cacccagtta aatgtttaga aactggaaag
    21901 gtatttataa cttctcaaaa tcatggatat aatgtttcag aaatgccaga aaatacagag
    21961 gtaactcaca taaatttaaa tgacaatact atagaaggaa tgagacataa ggaattacca
    22021 atatatagtg ttcagtacat tccagaatac acaacaaaag aagatttaga ttgtatattt
    22081 aataaattct tagcattaat atagatttag tattatttac agatagtaaa aaccatttag
    22141 ataaggagaa aaatttatgc ctaaattaga tagtataaag aaaactttag tattagggtc
    22201 aggaccaata ataataggtc aagctgcaga gtttgactat tcaggaacac aagcttgcca
    22261 gtctttgaaa gaggaaggga ttgaagttgt attaataaat agtaatccag ccactataat
    22321 gactgataaa gaagtggcag ataaaatata tatagaacca ttaacaatag aatttataga
    22381 aaaaataata gaaaaagaaa gaccagacag tttacttgca ggtatgggtg gacaaacagg
    22441 gctaaaccta gcagttgaac tttatgaaaa aggcatacta gataaatatg gagtaaagat
    22501 aataggtact tcagtagaat ctatcaaaaa aggtgaagat agagatatat ttagagaagt
    22561 aatgaaagaa ataaatcagc ctgtaatagt tagtgatata gttacagatt tacaagcagg
    22621 tcttgattat gcacttacaa ttggttatcc agtagttgta agaccagcat acacactagg
    22681 tggaactggt ggtggtatag ctgataatga ggaagaatta agagaaatac tttcacatgg
    22741 tctacaatta agtcctgttg gacaagtatt aattgaaaag agtataaaag gttggaaaga
    22801 aatagaatat gaggtaataa gagattcaaa gggaaattgt atagttgtat gtaacatgga
    22861 aaatatagac ccagtcggag ttcatacagg agacagtata gttgttgctc caactcaaac
    22921 tttaagtaac aaagaatgtg caatgcttaa aaaagcatct ttagatatat taaatgcagt
    22981 agaagttcaa ggtggttgta acgttcaatt tgcacttaat ccacatagtt ttgaatatgc
    23041 agttatagag ataaatccaa gagttagtag atcatcagct ttagcatcaa aagctacagg
    23101 atatccaata gctaaagttt catcaaaaat agctttaggt tatactttag atgagataga
    23161 aaatgcagta actaaaaaaa cttatgcttg ttttgaacca actatagatt atgtggtggc
    23221 aaaaatacca aaatggcctt ttgataaatt taaaaaagca aatagaaaat taggaactaa
    23281 gatgatggca actggtgaaa ttatgagtat aggaagtaac tttgaggctg ctattcttaa
    23341 agggataaga tcacttgaaa caggaaaata ttcattagtt cacacaccat ctgaggagag
    23401 aagcattgag gaattaaaga aaagagttgt agtacctgat gatgagagac tatttgactt
    23461 agctgaaatg ataagaagag gctataaagt agagatgata gaacaaatta caggtataga
    23521 taaatggttt ataaataaat ttaaatggat agtagagcaa gaagaaaaat taaaagttat
    23581 gaaaattgaa gatttaacta aagactatct tcttgaatta aagaaaaagg gattctctga
    23641 taaaggtata tctgacttaa tgaagataag tccagaaaaa ttatatgaat tgagaagtct
    23701 atacaatatc aaaccagcat ataaaatggt tgatacttgt gcaggtgaga tagatgctat
    23761 atctccttac tattattcaa cttatgagca atatgatgag gtagttgtta gtgataagaa
    23821 aaaggtgata gtattaggtt ctggtccaat aagaataggt caaggtatag aatttgacta
    23881 ttgttcagtt cattgtgtaa aatctttaag aaaaatggac atagaaacta taatcataaa
    23941 caataaccct gaaactgtaa gtacagactt tgatacatca gataagctgt attttgaacc
    24001 attaacagaa gaagaagttt taagtataat agaaaaagaa aagccagaag gtgtaatact
    24061 acagtttggt gggcaaacag ctataaaatt ggctaagttt ttacatgaaa aaaatatacc
    24121 tatactggga acagattttg atgatataga tgcagcagaa gatagagaaa aatttgatga
    24181 tttactagag agactagata taaatagacc aaaaggaaag ggtgtatggt cacttaacga
    24241 aggtattgaa gttgctaatg aattaggtta tccagtatta gttagaccat cttatgtact
    24301 tggtggtcaa ggtatggaaa taacttataa cgaagaaaaa ttatctcaat atctaca
//
LOCUS       DAGQWR010000057        24289 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.057, whole genome shotgun sequence.
ACCESSION   DAGQWR010000057 DAGQWR010000000
VERSION     DAGQWR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.057"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            18..2966
                     /locus_tag="MIW95_001931"
     CDS             18..2966
                     /locus_tag="MIW95_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3151259.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            2944..3408
                     /locus_tag="MIW95_001932"
     CDS             2944..3408
                     /locus_tag="MIW95_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY3151260.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3521..3823
                     /locus_tag="MIW95_001933"
     CDS             3521..3823
                     /locus_tag="MIW95_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3151261.1"
                     /translation="MGVTNVTKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            3885..5249
                     /locus_tag="MIW95_001934"
     CDS             3885..5249
                     /locus_tag="MIW95_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBY3151262.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            5284..5757
                     /locus_tag="MIW95_001935"
     CDS             5284..5757
                     /locus_tag="MIW95_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151263.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYINFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGDLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLRFRNKKFENKNI"
     gene            5929..6579
                     /locus_tag="MIW95_001936"
     CDS             5929..6579
                     /locus_tag="MIW95_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBY3151264.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTFKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6753..7685
                     /locus_tag="MIW95_001937"
     CDS             6753..7685
                     /locus_tag="MIW95_001937"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBY3151265.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEDSIFIG
                     KNQNPYQEVHHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYRKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            7959..8672
                     /gene="ispD"
                     /locus_tag="MIW95_001938"
     CDS             7959..8672
                     /gene="ispD"
                     /locus_tag="MIW95_001938"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBY3151266.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8669..9154
                     /locus_tag="MIW95_001939"
     CDS             8669..9154
                     /locus_tag="MIW95_001939"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBY3151267.1"
                     /translation="MRIGLGYDVHKLTENRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9249..9457
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9521..11236
                     /locus_tag="MIW95_001940"
     CDS             9521..11236
                     /locus_tag="MIW95_001940"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBY3151268.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDQCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11358..12803
                     /locus_tag="MIW95_001941"
     CDS             11358..12803
                     /locus_tag="MIW95_001941"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBY3151269.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKTKGVAVEVDDRDNYT
                     TGWKFNEWEMKGAPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13243..14724
                     /locus_tag="MIW95_001942"
     CDS             13243..14724
                     /locus_tag="MIW95_001942"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBY3151270.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLVEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEITEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            14945..15100
                     /locus_tag="MIW95_001943"
     CDS             14945..15100
                     /locus_tag="MIW95_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151271.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15245..16642
                     /locus_tag="MIW95_001944"
     CDS             15245..16642
                     /locus_tag="MIW95_001944"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBY3151272.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKIFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            16627..17040
                     /locus_tag="MIW95_001945"
     CDS             16627..17040
                     /locus_tag="MIW95_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBY3151273.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17147..17242
                     /locus_tag="MIW95_001946"
                     /pseudo
     CDS             17147..17242
                     /locus_tag="MIW95_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase (FAD)"
     gene            17548..18378
                     /gene="thyA"
                     /locus_tag="MIW95_001947"
     CDS             17548..18378
                     /gene="thyA"
                     /locus_tag="MIW95_001947"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBY3151274.1"
                     /translation="MSLADKIFIDMCNNILENGVSSDGEVVRAKWDDGQPAHTIKKFC
                     VVNRYDLSKEFPILTLRPTNLKAAIDELLWIWQKKSNNINDLNSRIWDSWADETGSIG
                     KAYGYQIGQKHKYKEGEFDQIDRVIYDLKYNPYSRRIITNIYNHNDLNEMNLYPCAYS
                     MTFNVTGNRLNAILNQRSNDILVANNWNVTQYAILVHMLAQISDLEVGELVHVIADAH
                     IYDRHIPLVKELINRESYDAPKLIINPNIKNFYDFRVEDFTLENYKSGPQIKKIPVAI
                     "
     gene            18391..18885
                     /locus_tag="MIW95_001948"
     CDS             18391..18885
                     /locus_tag="MIW95_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBY3151275.1"
                     /translation="MKVIVAVDLNWGIGYGGKLLQSIPEDMKFFKEKTVGNVVVMGRE
                     TFKSLPGKKALKDRVNIVLTRSKSFNDDRLIVCNSIDETLKELQKYENDKIFIIGGET
                     VYKQFLPYCNELYVTKIQKTYEADRFFPNIDIMSNWDLIKESEVKEYNNIRYVFSTYI
                     NNSL"
     gene            18912..20288
                     /locus_tag="MIW95_001949"
     CDS             18912..20288
                     /locus_tag="MIW95_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBY3151276.1"
                     /translation="MKKDIFVNRVSNALENDRGAVFVGSGISFSSTEVDWFKLLEPLV
                     NELDITLDNKSDDLPMIAQYIVNNYSGNRGPLVNEISKTFNRKFKLNKYHKYLATTKL
                     STIWTTNYDMLLEDAFYDFDTSVKVSDDSITRNRLEYDVEIIKMHGCISRSDWNEIVI
                     TQEDYDDFSINKPAISQRLSEDLINKSFLFIGYSYRDPNIRNIMINARRLSKKHTQQH
                     YIILKKLKDSDSNITQDDVVRQELWCNDLKRLGISTLFIDEYSELEELLREISQKSRG
                     KTVYVTGSHKESNMNARKLGTKLAKHKDITLLSGQSSGIGSEVVAAFTEECIKSKEDI
                     INRMKIFPNPYSADEKYSDDITLIPDLKKCRTKMLNSTQVVVVFSGGIGTEAEIDVAI
                     KQNCNIIPVITCKEDRENPAIKKILNYSDNMKNIEIKDATYYNKIVDEDGVVTMEEIY
                     ICISKIMN"
     gene            20264..21364
                     /locus_tag="MIW95_001950"
     CDS             20264..21364
                     /locus_tag="MIW95_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBY3151277.1"
                     /translation="MHIKNNELKHDIIKLKKRNGFIEVGKDKKTLVNCLIGANELEDY
                     KYEIKKIDCILNISEKPDIVSDLSTKRRKISESIWYKIVKDTPFVSATLPIYLVSKNN
                     NKIDSNELLDIIIEQMENGVGMITIHPTANKQIFEDSKNRMVPVTSRGGGMVIQDLIS
                     KNFSEDNVYLKILPEIIKYAQKHNVCISLGATFRSGNIFDSNDRAQITEIKSQIELAK
                     RIYKSNVGVIIESPGHSRPSDIIKISSILKKEGFPIMPLGPIPTDIAIGMDHIAAAIG
                     ATIMGIEGCANIISTVTREEHTGGRPSIESTVESIRTAKIAAHIIDIHQMNDINLDMS
                     VSKSRAKSNTCIFGKDTRYCDRCRELCPLCIR"
     gene            21407..21832
                     /locus_tag="MIW95_001951"
     CDS             21407..21832
                     /locus_tag="MIW95_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBY3151278.1"
                     /translation="MHRIASFSQQSQRYVKLEQFEYIIPPEIEKIEKAKELFINSMKK
                     YQEDYDKLVEILFENHYNKLIKGGKNEKTSKRQVEKKAIEDARYVFPNACETKMVFTI
                     NARSLFNFFEHRCYENGSCLEGTMTCGDIVQVRKKFKNL"
     gene            21845..22582
                     /gene="rlmB"
                     /locus_tag="MIW95_001952"
     CDS             21845..22582
                     /gene="rlmB"
                     /locus_tag="MIW95_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBY3151279.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            22585..23112
                     /locus_tag="MIW95_001953"
     CDS             22585..23112
                     /locus_tag="MIW95_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBY3151280.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            23177..23824
                     /gene="sigH"
                     /locus_tag="MIW95_001954"
     CDS             23177..23824
                     /gene="sigH"
                     /locus_tag="MIW95_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBY3151281.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            23898..>24289
                     /locus_tag="MIW95_001955"
     CDS             23898..>24289
                     /locus_tag="MIW95_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBY3151282.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVP"
BASE COUNT        10051 a         2519 c         4515 g         7204 t
ORIGIN      
        1 ggagggaaca attataaatg aggcaaagaa taaaaaactt aattgaaaat gaagataaga
       61 aaaatccttt aacagatgaa gcgatttcaa gtaggttaaa tattcgaaga gaagatgtta
      121 cctttttgag aaatgagtta aaaatagaag attcaagaca aagaagaaaa gttgttttaa
      181 ttaaagctat aaaagatatt ttaaaagaag aaaaaactat aaataaaaat gatataacaa
      241 gaagattaaa cagcattggg tttacagtat caagatttac agtcatacaa tacttaaaag
      301 agattcaaga ttctggtgac ctaaaacttg ataaagagag tgatgataaa aaagaagaaa
      361 taatcaataa tagagaaaat aaagatatag catttgataa actcataggt agtagtggta
      421 gcttaagtac aattataaaa ctagccaaag cagcaattct ttatccacct catggtcttc
      481 atactattat tatggggcca actggtgttg gaaagagtga gttagcagag tgtatgtata
      541 aatttgcagt agaatcatcc agattcccta agaatgcacc atttatagtt tttaatgcag
      601 cagattattc agagaatcca cagcttttgc tatcacagtt atttggacac gttaaaggtg
      661 cttttacagg agctgatgag aacaaagaag gattagtatc aaaggcagat ggaggaatat
      721 tgtttataga tgaaatacat agattacctc atgaaggtca agaaatacta tttcaactga
      781 ttgataaagg tatggtcaga aagttaggag aaacaagact aacacataaa atagatgtaa
      841 tgattatttc agcaacttct gagccaatag attctcattt acttaataca tttaaaagac
      901 gtatacctgt aacaattgaa ataccagaat tagttgcaag accattaaat gagaggtttg
      961 acattataaa taatttcttc ttaatagaag ctcaaagaat gggagcaaac atacatataa
     1021 agtcagatgt attaaaagct cttatgcttt acgattgtat aggtaatgta ggtcaactta
     1081 gaagtgatat tcaagtagct tgtgctaggg gactattaaa ccaacttaca aacaaactaa
     1141 aagaagtaaa tataagcata tcagatgtac ctggatatgt aaaacaaggt cttataaaaa
     1201 tcagaaattg tagaggtaaa atagaaaatt atgtagatgg ggatttaatt atagattcaa
     1261 atcttcaagg tgagatagaa aagaaaaatg aagatatata tacttttcca gatgaaatat
     1321 acaaacttac tgaaagaaga catattgatt tattggaaca aggtttagat tttgatgtta
     1381 taaatagaat cattggagga gaattagaaa gttgcattca aaaatacata aaacaagtta
     1441 aaaaagtttc tattagtaac aatcttccag atataagtcc aattgttgga aaatatatcg
     1501 tagagctaac ttatgaagta atcaaaatag catcacaata cctaagttca ctagaaccga
     1561 atctacattt atgtttagca gttcacctaa aagcagcttt tgagagatta aaagaaggaa
     1621 aagtaatatc aaatgctcat ttgaaagaaa taataggaaa ttatccacta gaatattctg
     1681 tagcagaaaa ggtagctcaa tatttaaagg aaagatataa tataaactta ccaaaagaag
     1741 aaataggtat aatagcaata tattttaaaa tgagtagtag aagagatatg tctgaacaaa
     1801 aaaagatagg tgtacttctt atggctcacg gaagagtagc atcttctgta tgtgaggtta
     1861 caaataaact attaggaatt aatcacgcaa ggtatttaga tatgccattt gagaagaagc
     1921 cggaagaaat gattgaagaa gctactacaa taataaaaga tattgatgaa ggtagaggtg
     1981 tgattatatt agtagatatg ggttcacttt catattttgg agatattatt tctggaaaga
     2041 ctggaattaa tataaggaca atatctagat ttgatacatt gctttctata gaggttatac
     2101 gaagagcagt tttaccagat ttaacaatag acgagatagt tgatgaagtt aaaggtatag
     2161 agccacaagt aaaagaaaag gttagtaaat gtaacaatac aaaagtagat tttagaaaac
     2221 ctataatttt aacattatgt attactggat tagggggagc ttgtaagata aaaaaaatcc
     2281 tagaggaaaa tattaaaagt ataactgaaa aggttgatat agaacctata ggtatgattg
     2341 aatatccaag tcttgattta gagataaaac aaataagcaa tgataaaaat gtaatcgcca
     2401 tagttggaac aattgaccct cagatagcaa atatcccttt ttttagtcta gtagacataa
     2461 atgataaagc aaagttaaat caaataaata acctaattga tacaaatcaa attggtgaaa
     2521 caaataaaaa atatgaatta tcagatttaa tacataaaga agatatattt gtggatttac
     2581 aggttaaatc aaaagaagaa gttctaaaat atatgagtaa aaaacttaga gagaaaggtt
     2641 atgtgacaaa aaatttctat aaaaaagtag tggaaagaga taacttattt ccaactgaat
     2701 tatcagatga aatagcaata cctcacacag atggaataga tgtaattagg ccagctataa
     2761 gtatagccat cttaaagaag cctattgtgt ggagtaaaag taaagtaaaa ataatacttt
     2821 tattagctgt agaccaaaag tgttttaatc cattaagcac tctactctca tttatagaaa
     2881 cagatgaatt taagaagata aaaaacatta aagatagaga ttttattagg gagatgatat
     2941 tagatggagt caatgaaaat aattagtagc aatttaattt ttaaaaatat tgaagtgtct
     3001 aacaatgaag atgcattaaa gtttttggga caacgtttgt ttgacgaaca gtatgtaaaa
     3061 gaaagctaca tacaagctgt tgtagcaaga gaaaaaaagt atgctacagg attaccaaca
     3121 gaaatatacg gagtggcaat accacataca gatatagtac atgtaaatga gccaggaata
     3181 gcaattggaa tactgaataa accagtaaaa tttataatga tgggtacaga cgatactgaa
     3241 attgatgtaa aagttgtatt tatgttagca gtgaaagagc cacaagaaca attgcaacta
     3301 ttagaaaaac ttatgactat atttcaagat aagaatatac taaataatat tgttgactta
     3361 agtgaagaaa gtgtgagtga tttattaaac tctaaattga aaaattaaac atgtaaagta
     3421 tattaaaaat gtaaaattaa acatgcaaag tatgttaaaa aatataaaaa gattattaaa
     3481 gatttctaac tatagatatc tttaaaataa aataatttca atgggggtga ctaatgtgac
     3541 taaaaagata ttagttgcat gtggaacagg agtatgtact tcaacaattg ctataaacaa
     3601 attaaagaaa gcacttcaag acattggtaa attggatatg gttaatatta cacaatgtaa
     3661 agtagtagaa gttgcatcaa aagcacctga ttatgattta ataatttgta ctacacaagt
     3721 atctagtagt ataaaaacac cagtgataaa tggattacct tttttaactg gtgtcggaat
     3781 ggataaatta atcaatgatg tattagaaga gttagaatta taattaaaaa aattaaggaa
     3841 catggtctct aaaatcataa gttttagaga cttaaaatta aataatggag ggtctttata
     3901 tggagatatt aaatttcatt gttggtctag gtgcacaagt aatgatgcca ataataatat
     3961 gtatttttgg tttgatatta ggtacaaaac taggaaaatc tttaagagct ggtttaactg
     4021 tgggagttgg gtttataggt cttaacacta ttatagcttt gctaacagat aatttaggtc
     4081 cagctacaca acaaatggtt aagaatttag ggctaagttt atcaataata gatgtaggat
     4141 ggccagcagc atcagctata gcttttgcat caacagtagg ggctttaatt attccaatag
     4201 gtcttgtggt aaatatagtt atgctaatta ctaatactac tcaaactgtt gatgtagaca
     4261 tatgggatta ctggcatttt gcttttacag gagctttagt ggcaggggct actcaaagtg
     4321 taatgtgggg agtatttgca gcagtagcaa atatggtaat tgtattggta atggcagatt
     4381 taactgctcc aggtatagag gaatatttag gtatgccagg tatatctctt cctcatggat
     4441 ttacacaggc atttgttcca attgctatag tagtaaataa attattagac ctaataccag
     4501 gtataaataa gattgagata aatgctgata cattgcagaa aaaatttgga ttatttggag
     4561 aacctttaat aatggggtct gtaataggtg taataattgg tattgctgcg aaatatgata
     4621 taaaaggaat attacaatta ggtgtaacta tgggagctgt attaatatta attccaaaga
     4681 tggcagcatt attaatggaa ggattattac cagtatcaga agctgcacag gaatttattg
     4741 agaagagatt taaaaacaga ggcaagattt atataggctt agattcagca gtaggaatag
     4801 gtcatcctgt tacattatca gttgcactag tgttggttcc tttgactata cttatagcag
     4861 ctatattgcc aggaaataaa gttttaccat ttgcagattt agcagtaatt ccatttgcac
     4921 ttgtattaat cgtaccaatt acaaagggaa atgtgtttag aacattaata ataggaataa
     4981 ttataattac ttctggatta ttaatcgcaa ctaacttagc tccattattt acccaaatgg
     5041 ctttaaatgc tagctttaaa atgcctgaag gagcaactat gatttcaagt atttgtgatg
     5101 gtgcgaatcc attaagttgg gtatttgtaa aagttatgaa ctataaagtt attggaggag
     5161 tagtttttgg agttatagct ttaggaatgg ctatttacaa taggaataga ataataaatg
     5221 aaaataaaaa gctatctata gaagaataag gttaaaacaa ttttattaag gagtgtatag
     5281 gttatgaaaa aatataaata tgattctaaa aggtacttac tcagtgttac aataccagga
     5341 ttttttatga ttttaatatt gatttatgca ctgtttaata actatataaa ttttaatttg
     5401 aatatatata gtttgttaat aattgtctgt acttatggta tattcaatac ttttatatca
     5461 tcaagtaatc ctaaaaaaat tattattgac agcaaatgta tacattttac ctcatttatg
     5521 actacacata agtatgaaat taaagatata gaaaagataa gaataagaga attttataat
     5581 aaaaaaattt atctaaaaat taatgatgga gaccttttta aaggtaggta ttggataaga
     5641 acaaatatgt ttaataatag tatagcatta tatagttatt tcattgagtt ggaagagtgc
     5701 ttatatccag attcactaag atttagaaat aaaaaatttg agaataagaa tatataaaaa
     5761 taaaaaattg aattgaagcc taacttaatc ttttttgata agatataagt ttgaacttta
     5821 tactaaattt ttagaatttt caatcaagac aataacataa tagctaagca attagaacac
     5881 aactaagatg tataaggcat gtatatgatg aatgggagga tttaattaat gttattagag
     5941 aaattaagaa aagaagtatt acaagcatct ttagaccttt taaactataa tttagtgaca
     6001 ttaacaggag ggaatgtaag tgggagagat gaacaaactg gctatatagc aatcacccca
     6061 agtggaatgg attatagaaa tcttacacca agtgatattg ttatagtaga tgtagatgga
     6121 aatattatag atggaaaatg gaaagcatca gtggatttat cagaccactt gtatatatat
     6181 aagcatagag aagacataaa tagtataatt catacacatt ctacatactc aagttgtttt
     6241 gctattttaa atgaacctat agaatgcgca agtacaactc ttgcaaatga agtagggggt
     6301 tcagtaccag ttgcaaaatt ttcaccacca acatttaaaa agatgggaaa atgtgttatt
     6361 gaagcaattg gtgacaaaag agcatgtctt ttagcaaatc atggcgtaat tgcagttggt
     6421 ccatctgtag gacatgctct tacagccgct gtaatgttag aagattcagc aaaagtatat
     6481 tacttagcaa aatcaattgg cacaccagtg ctactgcctg atgaagaaat tcaaagagcg
     6541 agagatgtat tttttaatgt ctatggtcag gataaataaa ttagataaaa ttttaaaagt
     6601 aatatgaaaa tatagatatt caatgatatt aaggagattt ataatgatat aataagttta
     6661 atagttaaga agtaaaatat gaaatgtaat agtatataaa ttcatttaag actaaaaata
     6721 ataagataaa ataaaaagga gtctgtattt ctatgtatta cttattaaac aaaagcacaa
     6781 atccttactt taatttggca ttagaagaat atctattttt aaatgataaa tataatgatg
     6841 atattataat catatggaga aatgaagact ccatttttat aggtaaaaat caaaatccat
     6901 atcaggaagt acatcatgat gttattgaaa agggagaaat tcctatttta agaagaatat
     6961 ctggaggggg cactgtttat catgatttag gtaatatcaa tatgtcattc atacaaaaag
     7021 ataggcaact tcatgagata gattttttgg agcatactaa gtttatgcaa aagatgcttt
     7081 caaccttagg actagatgta tctataactg aaagaaaaga tttattttta aatgggaaaa
     7141 aaatttcagg ctctgcacag tctataaaaa gaaaaaattc tctttatcat ggaactcttc
     7201 tttacgattc agatttaaat aaattaacta aatatttaaa ttcaaataaa gcaactgaat
     7261 caaatgcaac taaatctgtt tcaagtaaag ttacaaatat taagttttta ctcgaaaaag
     7321 atataaatta ctttttagat tattgtgttg aatatttaaa atctaatata aagaatataa
     7381 aagaacttga attggatgaa gaagatatta aaagtgtata caatatagga gaagataaat
     7441 atagaaagat agagtggaca tatggtaaaa cgccgaaatt tcaagtagtg ctaccattta
     7501 ataatgaaag taaaataaat atacatgtta acagatggaa agtatcaaaa ttttttattt
     7561 cttatgatga taatgtaatt aatatggata agttgttaga tttgaatttc tttaaagaag
     7621 aaataaaaga gagtatttta gaaaattatc cacattacat agatttaatc aatttaatat
     7681 tttaatataa aacctctaat ttgataaaat tgtcttatat caaactagag gtttttaata
     7741 ttcttgcata ttaacaacca ttacaaaatt gtaaaattaa aaacaacact attgaaaaat
     7801 caatatatat tgtaataaat atatgtgttt ttcattagca aatagacatt aactatttat
     7861 acaactgaaa atttaatata taattaatca gatggttaaa ataaaaatat taaaagtttt
     7921 aataaatgat ggttaaaaat aaaatacgag gtgataccat gtacagtgtt ataattgttg
     7981 cagcgggaag cggaaggaga atgaacttag atataaacaa acagttcata aaattaagag
     8041 aaaaagaaat tattgctcat acaattcaag ttttttatga aaatataaat atagatgaaa
     8101 tagtggtatg tataaaaaaa gaagaagagg atttttttaa agaaaatata ataaataagt
     8161 ataactttaa aaatataaaa atagcatatg gcggaaaaga aagacaggac tcaatatata
     8221 atggtttaaa aaaattagat gaaaattgtg atatagtact tatccatgat ggagcaagac
     8281 catttgtcga ccatagaata atcaacgaat ctataaaggt agctaaagaa aaaaaggctg
     8341 tggttgtagg tgttccagta agtgatacta taaagatagt gtcagatgga acagtacaag
     8401 aaactccaga aagaaatttg ttgtgggcag cacaaacccc tcaaaccttt gaatataatc
     8461 tcataataga tgcttatgag caagcatata agaacaatta ttatggtact gatgattcaa
     8521 tgttggtaga aaatataggt caaagtgtca ctatggtaat gggttcttat gaaaacataa
     8581 aaataactag tccagaagat ttaaatatag cagaacaaat attaaatatg gaaaaaagag
     8641 atgaagttaa cagtagaagg agaattatat gagaatagga ttaggatatg atgtacacaa
     8701 attaacagag aatagaaaac ttataatcgg tggtgtagag ataccacacg ataaaggttt
     8761 gttgggtcat tctgatgctg atgtattgat acatgcaata atggattcta ttttaggtgc
     8821 tctagcatta ggagatattg ggaagcattt cccagataca gatgaagaat ataagggagc
     8881 agatagtatg aaattattag aacatgtgta taatctaata acaagtaaag gatataaaat
     8941 tggtaatata gatagtacta taattgctca aagccctaag atggctccat atattgagag
     9001 tatgagaagt aatatatcaa aagttttaaa cacagacatt gataatataa acataaaggc
     9061 aacaacagaa gaaggtcttg gttttacagg agcaaaacag ggaatagctt cacaaagtat
     9121 ttgtttatta ttgttgacaa gtcagaataa ttaatatatt attagtaata agtttaatgg
     9181 atatcaagta ataggcctat atagaatatc tataatgtat gaatttaagt gaaattaata
     9241 taaatataaa attagctatg ataaagaaat agtaaaatga tgaaatcttc acagagagaa
     9301 aacggtggtg agagttttca agaggaagtc tttgaacctg tcttttagct taaaggtata
     9361 aaaatcttta cggtaggaac cgttatattc ccaaaagaga gctattcata tagctaacta
     9421 gagtggtacc gcggaaatta aacctttcgt ctctatactt gtatagagat gagaggtttt
     9481 ttatattttc aggaagttaa tattcaggag gaatctatta atgaaaatgt caaaaatgtt
     9541 tatgccgaca ttaaaagaaa tcccagcaga tgctgagatt acaagccatc aattaatggt
     9601 taggtcaggc atgataaaaa aaatgacttc aggtgtttat aaccaattac caatggggtt
     9661 aagagtgttc aaaaaaattg aacaaataat tagagaagaa ttaaataaaa aagattgtca
     9721 agaaatactt tgtgcagcct tacttccttc tgaattatgg aaagagtctg gaaggtggac
     9781 tgctatgggt gaagaaatgt ttagactaaa agacagaaca gaaagagagt actgtcttgg
     9841 gccaacacat gaagaagcat tcactgatat aataagacaa gaaattactt catataaaca
     9901 attgccactg aacttgtacc aaatacaagt aaaatataga gacgagagaa gaccaagatt
     9961 tggtgttatg agaactaaga cttttacaat gaaggatgca tatagttttg atgtagatga
    10021 taaaggttta gataagtcat atcaagatat gtttgatgca tatgtaagta tatttgatag
    10081 atgtggattg gagaatagcc ctgtacaggc agattcagga gctataggag gttctacatc
    10141 agccgaattt atggttaagt cagaagttgg tgaagatgaa gttgtatttt gtagtggatg
    10201 tgattatgca gccaatgtag aaagagcaga atcttgtaat ctagcatcac aaaaagaaga
    10261 aatgaaagaa ttagaagagg ttcatacacc aggagcagct acaataaaag agttagaaga
    10321 atttttaaaa acttctccag ataagtttgc taaaacttta gtatatgagg cagatggaaa
    10381 aacagttgta gtcgttgtaa gaggagatag agaagttaat gaaatcaaag tatctaatgc
    10441 tataggttct gttatagaat ttgctcttgc tacagatgat gtagtgagaa aagtaactaa
    10501 tgcagaagta ggatttgcag gtccaatagg tataaatgct gactatgtat ttatagataa
    10561 agaaattgtt gaacaaagaa acatagttgt tggggctaat aaaactgagt accatataaa
    10621 aaatgctaac tatggaagag attttgaagg aatagttgga gattttagaa atgttcaaga
    10681 aggcgaccag tgtatagtat gtggtaagcc acttgaaata gctagaggtg ttgaagtagg
    10741 acatatattt aaactaggaa ctaagtactc agaatctatg aatgcaaact tcatagacaa
    10801 agatggaaaa tcaaagccta tagtaatggg atgttatgga ataggtgttg aaagaacagc
    10861 agcagctata attgaacaac ataatgatga gaaaggtata atatggcctt tgtcagtagc
    10921 tccataccat gttgtgatag ttccagcaaa tatgaaaaat gaagaacaaa tatcaatagc
    10981 tgaaaatata tataatgatt tgcaagctat gggtgtagaa gttttattag atgacagaga
    11041 tgaaagaata ggtgtcaaat tcaatgattc agaattaata ggaataccta tgaggattac
    11101 agtaggtaaa aatattaatg aaggtaaagt agaatttaaa cttagacata aagaagataa
    11161 agagataatt gacatagaag aaataaatga aaaagtaaag gcagagttta tcaggaataa
    11221 tgttaggtta ggataataac ttaaataaga tattgtatag ataatcaaag ttgtacttta
    11281 tagacaccat tttatctata tataaggata taaaatggtg tttttttata aaacaaataa
    11341 atgaggaggt aattgaaatg gcaaaaaatg aaaaacaatt tgttgaagaa ataacaaaga
    11401 tggaagatga ttttccacaa tggtacacag atgtaataac taagactgac ttagttgatt
    11461 atgcaccagt aaaaggtttt atggttgtaa agccatatgg atatgcatta tgggaaaaga
    11521 tgcaagaatt tatggataaa aaatttaaag aaactggaca taaaaactgt tattttccat
    11581 tattaatacc agagagttta ttaaacaaag aagcagaaca cgttgaaggt tttgctccag
    11641 aagttgcatg ggtaacacat ggaggaaata aaaaattaga agaaagatta tgtgtaagac
    11701 ctacttcaga gactataata tgtacaatgt atgcgaaatg gttaaaatca tatagagaat
    11761 taccatatct ttataatcaa tggtgctcag ttgttagatg ggaaaaatca actagacctt
    11821 tcttaagaac atctgagttt ttatggcaag aaggtcatac attacatgaa acagcagaag
    11881 aagcacaaga agaaacaata caacaattag aagtatataa agcgttatgt gaagaattac
    11941 ttgcaatgcc agttgtagct ggtcaaaaaa gtgaaagtga gaaatttgca ggtggtgaaa
    12001 gaacatatac tatagaagct atgatgcatg atggtaaagc attacaatca gggacaagtc
    12061 acttcctagg tcaacacttt actaaggctt ttgatattac ttttgcagat agagaaggaa
    12121 atttagctaa tccataccat acatcttggg gagcatctac aagacttata ggtggactta
    12181 taatgactca tagtgataac agaggtttag tattaccacc aagagtagct ccaattcaag
    12241 ttgttatagt tcctatagct gctaaaaaag gaaatgtaat ggagactgtt gataaaatat
    12301 atgctgattt aaaaactaag ggagtagccg tagaagttga tgatagagat aactatacaa
    12361 ctggatggaa atttaatgag tgggaaatga aaggtgctcc agtaagagtt gaaattggac
    12421 caaaagatat agaaaataat caagctatgg tatttagaag agatacttta gaaaaggatt
    12481 ctatgccttt agaaggatta gctgatgcta tatgtgattt atttgatgtt atacacaatg
    12541 atatgtttga aaaagcaaga aaacatagag aagacaatac atctatagtt gaaaatatgg
    12601 atgagtttag aaaagcttta gaagaaaagc caggattcat aaagactatg tggtgtggag
    12661 atgcagaatg tgaagctaag attaaggaag aaacaggagc aactataaga tgcttacctt
    12721 ttgaacaaga aaacttaggt cacaagtgtg tgtactgtgg aaaagaagca gatagtatgg
    12781 tagtaatggc aaaagcttac taaaatacat ttaaataaaa actggggtag aaagtattta
    12841 atactttatc ccagtttttt tgttttttat gagtaataaa atgtatttgt aactatgtca
    12901 taagataagt catagatact tatattaaaa agaatataat agagaactat ttttaataat
    12961 tacatattaa tgaaagcaaa atatttttgt tatatagatt ttcaataaag caaacaataa
    13021 atataaatga atattagtaa ggagtgcttt taatttttag aaaataaatg acttaaaaga
    13081 aagatttatg aaattaaaac taggattttc tgatataata aacatgtgta tttttaaata
    13141 aaaaggatat tattattaga tttgattaaa aataaacttg caaaacaatg tactagttta
    13201 atattatagt tatattgaat aaaatatcag gaggaagtaa aaatgaatgt aagagtaagg
    13261 tttgcaccaa gtccaacagg atttgtacat ataggtagtt tgagaactgc tttatataac
    13321 tatttatttg caaaaaaaat gggtggagaa tatatattaa gagtggaaga tacagaccaa
    13381 agtagattag tagaaggtgc tatagagaat atgcttaatg ctatgaaatg ggctggtgtc
    13441 aaccatagtg aaggtgttat attagatgat agtggaaaag tagtacaaaa aggagaatat
    13501 ggtccatata ttcaatctca aagattagat atataccaag aatatataaa gcaactttta
    13561 gatagtggaa aagcttatta ttgtttctgt acaaaagaaa gactggatga agtaagagat
    13621 gctcaaagag cagcaggaga aacagctaaa tatgatggtc attgtaagaa tctttctaaa
    13681 gaagaagtag aagcgaatat aaaagcagga ataccatatg ttataagatt aagattgcca
    13741 gagaatcata caattaagtt tacagactta gttagaggag atatggaatt taatactaat
    13801 gacctagatg accaagtatt aatgaaaaca gatggatttc caacttatca ttttgcagta
    13861 gttgttgatg actatttgat gaaaattaca catgttataa gaggagaaga atgggtatca
    13921 tctactccaa aacatgttta tttatatgaa gcttttggat gggaagcacc tgtatttgtg
    13981 catttaccaa atatacttaa taaagaaaag aaaaaattaa gtaaaagaca aggtgatgta
    14041 gctgttgaag actttaagaa aaaaggatat ttaccagagg gattagtaaa ctatgtagca
    14101 ttagttggtt ggtctcctga agataatcaa gaattgttta caatggagga attagaaaaa
    14161 gccttctctg tagaaagagt atctaaaagt ggtggagtat ttgatactga aaaattaaat
    14221 tgggttaatc agcactatat aaaagatggt gatgatgctt atctaacaga cttagcaata
    14281 ccatttttag tagaagatgg atttataaca gaagaagaag ctactaataa atatgatttc
    14341 ttaaagagta tgatatcagt acttaaagag aaattacaat atgtaaaaga aattactgaa
    14401 catgcaagta tattttttgg agataaaata gaggtagaaa ctgaagaggg aagagagttc
    14461 ttaagattag aacatatacc tactttaata gatgctttga gagaaaaaat agaaaaaaca
    14521 gaggtattaa atgctgagtt tgttcaagct atgcttaaag aaatacaaaa agagtacaaa
    14581 ataaaaggga aaaatttatt tatgggctca agaataattt tgacagggca aatgcatggt
    14641 ccagatttac caaaagtaat ggaagtttta ggaaaagaaa cttgcttaaa tagaatagct
    14701 tatgtaaaaa ataatatatt atagtcataa aagtccgcta atttagaagc ggactttttt
    14761 tgtgttagta tttggtgaaa atatctgctt aagaaaatga aggtatttaa ggtaataaat
    14821 ttaatatata aaaagcaaag caaaaatata aataaaatat gaataataaa ctaataaaat
    14881 attaagcata gaagaaggga atctattatt ttagtttgat ttaattatta agggggtaat
    14941 tactatgtat agtaatattg atgatgttaa aaaggaatta aaggagctat gcttagaata
    15001 tgttacaatt ttagaaaaat taaaagatga aaaaatgatt acagaagaaa cttttgaaaa
    15061 gtgctcttct caaaaaaaaa tatttttaga agaacaatag aggatggtaa tagtattatt
    15121 attatgtagt ttgaaagata gtacgaaaaa tgatagtaat actattattt attgcaattt
    15181 tcaactataa tataatatat aatgttaaga agataattta taaaaataca gtgaggtgat
    15241 gagtgtgaaa gtttataata cattgactag aacaaaagaa gaatttgttc cattagaaga
    15301 aggaaaggta aaaatgtatg tgtgtggtcc aacagtttat aactatatac acataggtaa
    15361 tgcaagacca tttataatat ttgatactct aagaaggtat ttagagtata gaggatacga
    15421 tgtaacttat gtacaaaact ttacagatgt tgatgataag attataaata gaagtcatga
    15481 ggagggaata tctcctgaag aagttgcggc taagtatata aaagaatatt ttgtagactg
    15541 tgatgggcta gggataaaaa gagctactgt tcatcctcag gttacagata atatacaaca
    15601 gattatagag tttataaaag agttggaaga taagggatat gcatatgcag taaatggtga
    15661 tgtatatttt gatacaaata aatttgaagg ttatggaaaa ctttcaggtc aaaaacaaga
    15721 agatttagag gcaggggcta gaattgaggt taatgaccaa aaaagacatc ctatggactt
    15781 tgttctttgg aaggcaaaaa aagaaggtga gccaggatgg gatagccctt ggggagaagg
    15841 tagaccagga tggcatatag aatgttctgt tatgtctaag agatatttag gtgaaacaat
    15901 tgatatacat gcaggtgggc aagatttaac attcccacat catgaaaatg aaatagctca
    15961 aagtgaagct agaagtggaa aaatattctc aaaatactgg atgcataatg gatatataaa
    16021 tataaatgat gaaaagatga gtaaatcaaa aggtaatttc tttacagtaa gagatatatc
    16081 aaaattatat gatttagaga tagtaagatt tttcatgtta tcagcacatt atagaaatcc
    16141 agtaaacttt agtgatgaga tgttaaatca agctaaagca ggtcttgaaa gactatataa
    16201 tactaaagaa aaattagaat ttacgctaag taatttagta gaatctcctt taacagaaaa
    16261 agaagtagag cttgttaaag aattagatga ctttagacaa aaattcatag atgctatgga
    16321 tgatgatgtt aatacagcag acgcagtaag tgtaatattt gaattagcta agttaataaa
    16381 ttctaatgta gatgaaaatt cttctttgga gtttgctaaa aaatgcttag atgaatttaa
    16441 tgaacttaca ggagtattaa atattgtaaa taagaaaaag gatactgtat tagataaaga
    16501 cattgaagaa ttgatacaaa aaagaactga tgcaaagaaa aataaagagt ttcaattagc
    16561 tgatgatatt agacaacaat tactagatat gggaattgta ttagaagaca caagacaagg
    16621 tgttaagtgg aaaaggatat aaaaatggaa aaaactgaat tagttactat gtcaccatta
    16681 gttttagcat acttaggtga tacagtgtat gagacatata taagagaata tttaattaga
    16741 cagaatacac aaagaaaagt taatgatttg cataaactag ctataaagta tgtaaaagct
    16801 aaagctcagg caactataat tcatgaaatt gaaattgagt taactgaaga agagagtaaa
    16861 atttataaaa ggggaagaaa tcaaaagtca aatacttctc ctaagaatgc agatattata
    16921 gactacaaac atgcaactgg gtttgaggca ttagtgggat atttgtattt aaataatgaa
    16981 atagagagac ttcaatatat tatcaataaa ggaatcaaaa ttattgaaag agatatgtaa
    17041 atattaaaga gtgttgtctt aataataagt aataaggcaa cacttttttt ataactaatg
    17101 tataataaat atatagaaat tagtaatgag aaaggataag aaagttatga aagtaaaatt
    17161 aatatcacat acacctgaac cagaaaaagt aatagctatg gcagctaagc tatgttattc
    17221 accagttggg acagacgaaa tagaaaaaga tttctaaaat atattaaaga gcattcctat
    17281 aacaaatact atggcggttg gaatctatga gaagttatag atatagaatt atgtaaatta
    17341 tttggaaaaa acaggtttga aaattagcca tgttggtatt tctaggttac ttaaggaaga
    17401 tcaaacataa ataagctgag ttgtcttagg tgcactatgt acaacattag aatgtgaagt
    17461 tgaagattta ttacttcaaa cgaacgatgg tactaagaaa aacaatgatg aaatgattaa
    17521 ttaataataa aggtaaggag gtagtatatg agtttagcag ataaaatttt tattgatatg
    17581 tgtaataata ttcttgaaaa tggagtatca tcagatggag aggtagttag agcaaaatgg
    17641 gatgatggcc agcctgctca tacaataaag aagttttgtg tagtaaatag gtatgatctt
    17701 tcaaaagaat tcccaatact tacactaaga ccaacaaact taaaagcagc aatagatgaa
    17761 ttactttgga tatggcaaaa aaaatctaat aatataaatg atttgaatag ccgtatttgg
    17821 gatagctggg ctgatgaaac aggttcaata gggaaagctt atggatacca aattggacag
    17881 aaacataaat acaaagaagg agaatttgac caaatagata gagtgattta tgacttgaaa
    17941 tataatcctt atagtaggag aattataact aatatatata atcataatga tttaaatgaa
    18001 atgaatttat atccttgtgc atatagtatg acatttaatg taactggaaa tagattgaat
    18061 gcaatattaa atcaaagatc aaatgatatt ttggtagcta ataattggaa tgtaactcag
    18121 tatgcaattt tagttcacat gttagcacaa ataagtgatt tggaagtagg cgaattggtt
    18181 catgttatag cggatgcaca tatatatgat agacatattc ctctagttaa agagcttatt
    18241 aatagagaat cttatgatgc accaaaatta ataataaatc caaacataaa gaatttctat
    18301 gactttagag ttgaggactt tacattagaa aattataaaa gtgggccaca aatcaaaaag
    18361 attcctgtgg caatttagaa ggagttattt atgaaagtta tagtagctgt agatttaaat
    18421 tggggaattg gttatggtgg aaagttacta cagtcgattc ctgaagatat gaaatttttt
    18481 aaagaaaaaa ctgtaggtaa tgtagttgta atggggagag aaacctttaa atcattacca
    18541 ggaaaaaaag cattaaaaga tagagtgaat atagtcttga ctagaagtaa atcttttaat
    18601 gatgatagat tgattgtttg taattctata gatgaaactc ttaaggaact acaaaaatat
    18661 gaaaatgata aaatttttat aataggtgga gaaacagtat ataaacaatt tttaccttat
    18721 tgtaatgaac tatatgtaac aaaaatacag aagacatatg aggcggatag atttttccct
    18781 aatatagata ttatgtcaaa ctgggatttg attaaagaaa gtgaagtaaa agaatataac
    18841 aatataagat atgttttttc aacatatata aataatagtt tataggtttg tataggagag
    18901 aaatttaatt tatgaaaaag gatatatttg taaatagagt ttctaatgct ttagaaaatg
    18961 atagaggcgc agtttttgta ggtagtggta tatcgtttag ttcgactgaa gttgattggt
    19021 ttaaattact ggagccatta gtaaatgaac ttgatataac attagacaat aaaagtgatg
    19081 atttacctat gattgctcag tacatagtaa ataattattc aggaaataga gggcctttag
    19141 taaacgaaat atcaaaaaca tttaatagaa agtttaaact aaacaagtat cataagtatt
    19201 tagcaacaac taaactttca acaatatgga caactaatta tgatatgttg ttagaagatg
    19261 cattttatga ttttgataca tcggttaaag taagtgatga ttctattaca aggaatagac
    19321 tagaatatga tgtggaaatt ataaaaatgc atggatgtat ttctagatcc gattggaatg
    19381 aaatagttat aactcaagaa gattatgatg atttttcaat aaataaacca gctatatctc
    19441 aaagattaag tgaagattta ataaataagt cttttctatt tattggatat agctatagag
    19501 atccaaatat aagaaatata atgataaatg ctagaaggct aagtaaaaaa catactcaac
    19561 aacattatat aattttaaaa aagttaaaag atagtgatag taatattacg caagatgatg
    19621 tagtaagaca agaactttgg tgcaatgatt taaaacgttt gggaatttca actttattta
    19681 tagatgaata ctcagagtta gaggaacttt taagagagat aagtcaaaaa tcaagaggta
    19741 aaacagtata tgtgacaggt tctcataaag aaagcaatat gaatgcaaga aaattaggaa
    19801 ccaagctagc aaaacataaa gatattactt tactaagtgg tcaatcatct ggtattggct
    19861 cagaggtagt tgctgcgttt actgaagagt gtattaagag taaagaagat attataaata
    19921 gaatgaaaat atttcctaat ccatattcag ctgatgaaaa atactctgat gatattactt
    19981 taataccaga tttaaagaaa tgtagaacta aaatgttgaa ttcaacacaa gttgtagttg
    20041 tatttagtgg aggtataggt actgaagcag agatagatgt tgctataaaa caaaactgta
    20101 atataattcc tgttataaca tgtaaagagg atagagagaa tccagctata aaaaaaatct
    20161 taaactatag tgataatatg aaaaatatag aaataaaaga tgctacctat tataataaaa
    20221 tagtagatga agatggtgtt gtaacaatgg aggaaattta tatatgcata tcaaaaataa
    20281 tgaattaaaa catgacatta ttaaattgaa aaaaagaaat ggatttattg aagttggaaa
    20341 agataaaaaa acgttggtaa actgtttaat tggagcgaat gaattagagg attacaaata
    20401 tgaaataaaa aaaatagatt gtatacttaa tatatcagaa aaaccagata tagtttcaga
    20461 tttaagtact aaaagaagaa aaataagtga atctatatgg tataagatag ttaaagatac
    20521 acctttcgta tctgctacac tgcccatata cttagttagt aaaaataata ataaaataga
    20581 tagtaatgaa ttattagata taattattga gcagatggaa aatggagttg ggatgataac
    20641 gattcatcca acagcaaata aacaaatttt tgaggattct aagaatagaa tggtacctgt
    20701 cacatctaga ggtggaggta tggtaataca agatttaatc tcaaaaaatt tttcagaaga
    20761 taatgtgtat ttgaaaatat tgccagaaat tataaagtat gctcaaaaac ataatgtttg
    20821 tatcagtcta ggtgcaacat ttagatcagg aaacatattt gattctaatg atagagcaca
    20881 gataacagaa attaagagtc aaatagaatt agctaagagg atatataaaa gtaatgtcgg
    20941 ggttattatt gaatcaccag gtcattcaag accaagtgac ataattaaaa tatcgtcaat
    21001 attaaaaaaa gaaggtttcc caattatgcc actaggacca ataccaacag atattgcaat
    21061 aggaatggat catatcgctg cagctattgg tgctacaatt atgggtatag aaggatgtgc
    21121 aaatataata tcaactgtca cacgagaaga acatactgga gggagacctt ctattgaatc
    21181 cacagttgaa tctataagaa cagcaaaaat agctgctcat attattgata tacatcaaat
    21241 gaatgatatt aacttagata tgagtgtatc taagtctaga gctaaatcta acacctgtat
    21301 atttgggaaa gatacaagat attgtgacag atgtagagaa ctgtgtcctt tgtgcattag
    21361 ataaaaatta tattcaagtt aattcaataa aaaagattgg ataaaaatac atcgtatagc
    21421 tagtttttca caacagagtc aaagatatgt caaattagag caatttgaat acataattcc
    21481 tccagaaata gaaaaaatag aaaaagcaaa agagttattt ataaattcaa tgaaaaaata
    21541 ccaagaggat tatgataaat tagtagagat actatttgaa aatcattata acaaactaat
    21601 aaaaggtggt aaaaacgaga aaacatcaaa aagacaagtt gagaagaaag caatagaaga
    21661 tgctagatac gtatttccaa atgcgtgtga aacaaagatg gtatttacta taaatgctag
    21721 aagtttgttt aatttctttg aacatagatg ttatgaaaat gggagttgtc tagaaggaac
    21781 tatgacttgt ggagatatag ttcaagttag aaaaaaattt aagaacttgt aggaggtgaa
    21841 taatttggcg agtatagaag gaagaaatcc ggtaatagaa gcaataaaaa gtgacagaga
    21901 aatagataaa atattaattg caaattcagc taaagaaggt tcaattaaga aaataatagg
    21961 aatggctaaa gataagaata taattattca gtatgttgat aaacataagc tagatgaagt
    22021 aagtacaagt cattcacatc aaggtgtaat agcttatgca agtgagtata agtattatga
    22081 attagatgag ctaatagatt tagcaaagaa taaagatgaa gacccatttt tcattatatt
    22141 agatgagata acagacccac ataatttggg ttcaataata aggacagcag atgcagtagg
    22201 agcacatgga gttatcattc caaaaagaag gtctgtacac ataactcctg tagttgccaa
    22261 agcatctgct ggagcagtag aatatatgcc agtttgcaag gtaaccaata tagttaatac
    22321 aataaaaaga ctcaaagaag aagggctgtg gattgctgca gctgatatgg atggagaaac
    22381 tttttataaa caaaatctta ctggtcctct tggtgtagta attggaagtg aaggttttgg
    22441 aatatctaga ttggttaaac aaaactgtga ctttatagta aaaatgccaa tgataggtaa
    22501 tgttacatct ttaaatgcat ctgtagcagg tggaatactt ttatatgaga tattcagaca
    22561 aagactggat aaaagtaaat aggcatgaaa aaaaatataa atcattactt aataatagat
    22621 ggctacaata ttataaatgc gtgggacaat ttgaaagaac tggctaaaga ggacttagaa
    22681 gattctcgag aaaagcttat tgatgacatt atagaatttt ctgagtttat gggttataag
    22741 actataatag tttttgatgc ttataatgtt aaaaattctc gagaaaaagt tgaaaaaaga
    22801 aaacacataa caatagttta tacaagagaa caccaaacag cagatagtta tatagagaaa
    22861 tttataactt ctttatcaaa gtatgatgat gtaaaagtag ccactaatga ctatgcagaa
    22921 cagcaaatga ttttaggaaa aggtgctact agaatgtctg caagagaatt aaagttagag
    22981 ttagatcgtt ccaagaataa aatgaaggaa aaaaatatcg gtttgaggaa aaaaattcag
    23041 cgaaattggt tggaggaaag gttagataaa gaaacattgt cgaaacttga gaacattcgt
    23101 agaaagcgtt gaaattacta ggttctttag actataatat acctataaat ttgttttata
    23161 tatgggggag atttacatgt tggtagcaaa agaaaaaagt tatgagttag tggacaattg
    23221 tcagcaggat gagtataaca ttgtattaag agcaagtgaa ggggataaaa tagcactaga
    23281 gtacattatt actaaataca gaaattttgt aaaggcaaaa gcaaagtcat attttttaat
    23341 tggagcagat aaagaagata taatacaaga aggaatgata ggcctttata aggcagttag
    23401 agattttgat ggaagtaaga ccaattcatt taaatgtttt gcggagatat gtataacaag
    23461 acaaataata actgctataa aaactgcaac aagacaaaaa catatacctt taaattcata
    23521 tgtttctttg aataaaccaa tatatgatga agagtcagac agaacacttt tagatattat
    23581 agctacaagt atagtaacag atccagaaga gcttataatt agtaaagaag agttaaaaaa
    23641 tatagaatca aaaatgaacg aattgttaag tgacttggaa ttagaagtac tagaattata
    23701 tttaaatgga aagtcatacc agtttatagc ggacaagctt aaaagagatg taaagtcaat
    23761 agataacgct ttacaaaggg ttaagagaaa attagaaaaa catcttgaaa acagaaatga
    23821 ttaatagtaa aatacttacc ataagcttat tgtgagcaaa attaatttat atcgaaagat
    23881 aagggaggaa attcaaaatg gctaaagcta aatacgaaag aacaaaacct catgttaata
    23941 tagggacaat aggacacgta gaccacggta aaactacatt aacagcagca ataacaaaaa
    24001 cattatatga cagatatcaa ttaggagaag cagtagattt cgcaaacata gataaagctc
    24061 cagaagaaag agaaagagga atcacaatat caacagcaca cgttgagtat gaaacaccaa
    24121 atagacacta tgcacacgtt gactgcccag gacatgctga ctacgttaag aacatgataa
    24181 caggagcagc acaaatggac ggagcaatat tagtttgttc agcaacagat ggaccaatgc
    24241 cacaaacaag agagcatata ctattatcaa gacaagttgg agtaccata
//
LOCUS       DAGQWR010000058        24270 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.058, whole genome shotgun sequence.
ACCESSION   DAGQWR010000058 DAGQWR010000000
VERSION     DAGQWR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.058"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..532)
                     /locus_tag="MIW95_001956"
     CDS             complement(<1..532)
                     /locus_tag="MIW95_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBY3151283.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYIL"
     gene            complement(645..1319)
                     /locus_tag="MIW95_001957"
     CDS             complement(645..1319)
                     /locus_tag="MIW95_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBY3151284.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            1522..3396
                     /gene="asnB"
                     /locus_tag="MIW95_001958"
     CDS             1522..3396
                     /gene="asnB"
                     /locus_tag="MIW95_001958"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBY3151285.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEYVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(3488..4924)
                     /locus_tag="MIW95_001959"
     CDS             complement(3488..4924)
                     /locus_tag="MIW95_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBY3151286.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVHFTRY
                     IGVDDSIDKYIEKVKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIAQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            5253..5831
                     /locus_tag="MIW95_001960"
     CDS             5253..5831
                     /locus_tag="MIW95_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY3151287.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            5870..6448
                     /locus_tag="MIW95_001961"
     CDS             5870..6448
                     /locus_tag="MIW95_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY3151288.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLRHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            6534..7163
                     /locus_tag="MIW95_001962"
     CDS             6534..7163
                     /locus_tag="MIW95_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY3151289.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            7153..9912
                     /locus_tag="MIW95_001963"
     CDS             7153..9912
                     /locus_tag="MIW95_001963"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBY3151290.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            9999..11561
                     /locus_tag="MIW95_001964"
     CDS             9999..11561
                     /locus_tag="MIW95_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBY3151291.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDIHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            11562..12698
                     /locus_tag="MIW95_001965"
     CDS             11562..12698
                     /locus_tag="MIW95_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBY3151292.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            12762..13838
                     /locus_tag="MIW95_001966"
     CDS             12762..13838
                     /locus_tag="MIW95_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBY3151293.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALYSMNLQDMYVI
                     PIIETREFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            13816..15054
                     /locus_tag="MIW95_001967"
     CDS             13816..15054
                     /locus_tag="MIW95_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBY3151294.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGVRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELNCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKKHYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            15108..16196
                     /locus_tag="MIW95_001968"
     CDS             15108..16196
                     /locus_tag="MIW95_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBY3151295.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            16224..17015
                     /locus_tag="MIW95_001969"
     CDS             16224..17015
                     /locus_tag="MIW95_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBY3151296.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKPDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDFSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFKGMSGTEEILSNILDENCLLNI"
     gene            complement(17130..17684)
                     /locus_tag="MIW95_001970"
     CDS             complement(17130..17684)
                     /locus_tag="MIW95_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBY3151297.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            17879..19081
                     /locus_tag="MIW95_001971"
     CDS             17879..19081
                     /locus_tag="MIW95_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBY3151298.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            19138..20118
                     /locus_tag="MIW95_001972"
     CDS             19138..20118
                     /locus_tag="MIW95_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBY3151299.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            20365..21318
                     /locus_tag="MIW95_001973"
     CDS             20365..21318
                     /locus_tag="MIW95_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBY3151300.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAVFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            21308..22264
                     /locus_tag="MIW95_001974"
     CDS             21308..22264
                     /locus_tag="MIW95_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBY3151301.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            22261..23016
                     /locus_tag="MIW95_001975"
     CDS             22261..23016
                     /locus_tag="MIW95_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY3151302.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            23108..24073
                     /locus_tag="MIW95_001976"
     CDS             23108..24073
                     /locus_tag="MIW95_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY3151303.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKTSLPASLEKYKDKKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         9570 a         2544 c         4148 g         8008 t
ORIGIN      
        1 ataaaatata aaattgatat ccaatcaaaa aaataactat gaaatttaat agcttagata
       61 tgaatttttc tgtttttgag ttagttttta taggtagtaa agaaatgttt atggtaatgg
      121 gataaaacaa ttcaatacct tctttagtaa taaggtcaag acacaagtga gatagatatc
      181 ctattataaa cccactggac aaacatgaga aaactatatt attgttagtg aagattgtca
      241 gagatttaaa aaaactataa ataagtaata aggcaattag actgtgagta aaccctctat
      301 gtctaaacct acttccaaat aatttgtata ttaacataaa ttttttactt atgtaagaac
      361 ctctcatatc tatatctgga aataaagaac ctaaatatgc aaaataaata taaattataa
      421 ttaatactat tttatatgca atatcgtatt taactaggta cagattgttt ataaagggga
      481 gtatcaaaag tgcgaatata taacctcccc tttcatgtgt ttcttttatc atataaaaat
      541 cctcctgaat cgtcactata aacattatat tggtaaattt agactatctt ttattatatt
      601 atatagcaaa cataagtttt tttcaaaagg attttaatat ttaattaatc ttgagatttt
      661 aaatttttgt aattttctaa agcaccattc caatttacta cattaaacca gttgtcaatg
      721 tattcatttc ttctattttg atattttaaa tagtaagcat gctcccaaac atcaagtcct
      781 attataggag ttaggttttt acttacaggg ctatcctgat ttggagtagt cataatagat
      841 aatttcccat ctttagtagc tacaagccaa gcccaaccag aaccaaagac atctaaagca
      901 gatttttgga actcttgctt aaatttttca aaagaaccaa agtctctatc aatagcttct
      961 tttaaagatt cagaaggtat ggttttttct ggcgtcatta tatcaaaaaa gaatttatga
     1021 ttataagctc cacctgcatt atttcttaca gttgtagcaa tatctttagg taaagaatct
     1081 aaattttgca ataattcaca taaagaataa ttataaagct caggatattt ttcaagagca
     1141 gcatttaatt tatcaacata agcttgataa tgcttatcat gatgcagttt cattgtttct
     1201 ttatctatat aaggttcaag tgcatcatat gcataaggta atggtttaac cttaaatttg
     1261 ttattttcag gtgtaaaagc aaatgaagtt atgcactgtg agattataaa taaagacata
     1321 ataactggta ttaatatttt tttcttcata aaaatcacct cctaatatag tatttctaga
     1381 aataaaaaaa atatgaaata ttaacagata atttataaga atttaatagc aatttcattt
     1441 taaattgaat aagttttaaa gccaaagaca aaactatgaa atatagaata agactaaagg
     1501 acagaaagga attttgatta tatggacggc aatatttcaa tttattataa aggtgatata
     1561 tctaaaaaag aagatattaa atttaaaata aaagaatctg taaataatac aagactttct
     1621 agtagaatag agtataagga tgaggaaaaa acttttgatt ttgaaagatt taatgtaatt
     1681 gatgctattt atggaaataa accatttgaa aaagatggaa aagttattgt ttttaatgga
     1741 aatatatata actatcatga aataaaagaa gaattaatta aaaaaggaca caattttact
     1801 acaaatgatg atattgaagt cttattggca tcttatatag aatttggtaa gaattgtgta
     1861 cataagatta aaggaatgtt caactttata atctatgata gagaaaatga aagtatattt
     1921 ggagcaagag atttatttgg gataaaacca ctttattata taaataagga aaatgcaata
     1981 attttttcct cagagtataa gtttttactt gaatatatga aaaatttaaa tataaatgaa
     2041 agaagcttac aaagttattt ttcatttcaa tatgtacttc ctgaagatac tatgatacaa
     2101 ggaataaggc tgataccagc aggtcactat tttagggtag aaaatggtat actatcttta
     2161 aagagatata ataaacttga gtttagaagt agtacaaaat ttttttatac taaaaatcac
     2221 ttaggcaata gagatgtgaa tgaggatgat ataagaaata tagtagtaga ttcaatagta
     2281 actcatatgg aagaggagaa agaaattgga acatttttgt ctggtggaat agattcatct
     2341 attataacta ctgtggcttc acaaattaat ccaaatataa aatctttttc tgcaggcttt
     2401 agtgtcaaag gatatagtga attagaagta gcaaagaaaa cagctgataa gttaggtata
     2461 gaaaatatcc agattaatat aacacaagat gaatatataa aatcattacc gaatgtaatt
     2521 tattctttgg atgaccctat agcagaccct tcagaagtag gtctttattt cttaattaaa
     2581 gaggcaggta aacatgttaa agtagcattg tctggagaag gagcagatga attatttgga
     2641 ggatataata tatataaaga gtatagtact atgaaatcag ttgtcaattc acctacttat
     2701 attaaaagta tattaggaag agtttcagaa cttatgccaa acataaaagg aagaaattat
     2761 ctttatagag ctacaacacc attagaaaaa agatatatag gaaatgcaaa agtttttgaa
     2821 aacagcgaag taaaaagatt cttttttaag tataaggaaa agaatatata tgaatacctg
     2881 ttgtctaatt tgtatagaga tgcacaaaaa aataattacg attatataag taaaatgcaa
     2941 catatagatg tgaatacatg gcttcaaggc gatatacttc aaaaaattag caaactttct
     3001 acagctgagc aggttgaact tagagtacca tttttatata aagatgtatt tgatgtagca
     3061 aaaaatttaa gaatggagca aaaaataaat aagaataata caaaagtttt acttagagag
     3121 gcgtttagag aaatagtccc agaatatgtt gttcaaagga aaaaattagg atttccaact
     3181 ccaataagag tttggttaaa agattctcta ggggatattg taaaggaaac tatttataat
     3241 tcaaatgtag atgaatttat agataaaaaa tatgcaataa aacttttaga tacgcatctt
     3301 aaaggtcata gagataattc gagaaaaata tggactatat ttacattttg tctatggcat
     3361 caattattta ttgagcataa aaatgtcgag tattaatact tttttcaata ttaatcagag
     3421 ggcaaaagca tgcaatgttt tcaccctctt gattattatg gaataggata agtagaattt
     3481 tgattgatta gtttactatc tcaataaaat tattggcaaa tattttgaca agattacttt
     3541 caacagctaa atttaagttt tgtccatcat catatccagc ggaaatcaat cctagtaagt
     3601 ttccaaacat atctaaaaga gctcctcctg aactaccact agagattgga gctgtaaatt
     3661 gtatcatttg aaccgtttca aaatcacgaa aagctgagat aattccatct gaaactgtat
     3721 taaaaagacc taaagggctt ccaattgcaa ctattttttg acctctcaca ggttttttac
     3781 tgactttaac aggtattggt ttgcattttc tatcaacctt tatcacagct aaatcataat
     3841 ctgaatggta ttttataatt tggtaagatg tataaacttt atcatcattt tcaaatctaa
     3901 ctaaaaatga atacccatca ttgactacat gaagattcgt aagtatataa ccttcattat
     3961 ttattactac tccagaacca ctcttaaacg gtttattttc gttattgcac acaacaatca
     4021 taacaacaga agctgataac tttgcaaggt cttcataaga aagttctact tcctcatctt
     4081 gtgcaatttt tttagatgtt agatttgtgt tattaagtgt atttttagag ttaggtttat
     4141 tcgtagtagg agaagtctta ttggtatttt cactgtaatt tatatttgta atatgccttt
     4201 caaaaggtat ttctaatata tttggagttt tcctagaaag ttcaaataga gtagaaaatt
     4261 ttgaacattc aggatataaa tcaagaatat ctttatttgg atttatatat aaaatatcac
     4321 acccaagttg tgataaaaaa tataagaaaa aaagttcttg ccttttaata tttccaaagt
     4381 atactatctt tggtgaaata gtagtcttca ccttgttata aaataattta ggtagaaaat
     4441 agtccatcca acacaaaagt ttaactgcaa agtttttgat gatagaggat gtacaatttg
     4501 gcatacttgt ggaaaataaa tctaaagtat ttttaaatgc aatcttttta gtccaattta
     4561 aagtgtcatt ttctatttta catggaaagt cgatattata taaatcccaa ctgttaaact
     4621 tagaactatc ataaatatta aaaattttaa gcatcttatc tgtttgttct ttgtcaaaat
     4681 ttatttttag attgtttaat ctaatatagg gaattgtagg attattaaaa aaataagtat
     4741 ctatattttt aactttttct atgtatttat caattgaatc atcaacacct atatatcttg
     4801 taaagtggac ttctttaata tatgaagaac tcttattttt ataggtagta tcagatataa
     4861 attcaattag aggttcagtt gactttttta ttgaaatctt taatggttta atagtacaaa
     4921 tcatattttt tctcctataa taagatgata taatctatta taaaacaact taaaacaata
     4981 tagtacaata attttgtagt ttcaaaataa aatatgtact tgtttatata aatgatatat
     5041 aattacatat taaacatagc ttttatacat ttaataaagt atgtaaaatc tatgtaaaaa
     5101 gtaagaaata taaacatttt tttaattata ttttaaaaat atcaagcaag ttttcttcac
     5161 aaatgaataa aaaatgttat aatatgatta aagaaaaaga taaataatta gcaagatttg
     5221 tttagctaaa attaacaagg gagcgataaa ttatgggtat aacattatca aaaggccaaa
     5281 aggttgattt aactaaagga aatccaggtc ttaaaaatat tttagtaggt ttaggatggg
     5341 atactaacaa gtatgatggt ggatttgact ttgatttaga tacagcagca tttttaacag
     5401 gagcaagtgg aagtgttact aatgatggag attttgtatt ttacaacaat ttaaaacata
     5461 catctggagc agttgagcat ctaggagata acagaactgg cgaaggtgat ggagatgatg
     5521 aacaaatagt tgttgattta tctaagattc ctggagaaat aagcaagatt tcttttactg
     5581 ttacaattca tgatgcgaca gaaagaagac aaaattttgg acaagtaagt aattcttata
     5641 taagaatagt agataaagac acaaatgaag agttaataaa gtatgaatta ggagaagatt
     5701 ttagtataga aacagctata gtagtagctg aaatatataa gcacaatgga gaatggaagt
     5761 ttaatgcact aggttctggt tttgaaggtg gtcttgctgc attatgtgga aactttggaa
     5821 taaacttata atttcaacta taacaataaa ctaaaggggg atattttcca tggctatagt
     5881 attaaaaaaa ggacaaaaaa tcgatttaac taagggaaat ccaggtctta aaaatataaa
     5941 acttggacta ggatgggata caaattcatt tgatagtggc tatgattatg atttagatgt
     6001 aagtgttttc atggttggag aatctcaaag agttgaaaaa gatgaagatt ttatattcta
     6061 caataactta agacatccat ctggagcagt tgaacactta ggtgataata gaactggtga
     6121 aggtgatgga gatgatgagg aaatattagt gaatttagag acaatgccaa agcatataca
     6181 aaagatagct gtaacagtta caatatatga ggctgctgaa agaagacaaa attttggaca
     6241 agtaagcaac tcatatataa gagtattaaa ttctgaaaat gatgaagaaa tattaagata
     6301 tgacttaggt gaagaattta gtatagaaac tgcaattaca gtatgtgaga tatataagta
     6361 taatggagaa tggaagttta gtgcagttgg agcaggattt gaaggtggct tagaagcact
     6421 ttgtagaaac tatggcttaa atgtttaatt taagcaatat aatatattaa atataaataa
     6481 cctatacaaa taatgtatag gttatattat ataaaatttt gtggaggttt agtatgtcga
     6541 ttaatttatc aaaaggtgat aaaatagatt taaaaaaatc taacccagga ctgagtaaca
     6601 tacttgtagg tctaggatgg gaccctgtgc aacaatctgg aggaggtttt tttaaatcgt
     6661 tatttggtgg aggacaagct gatatagatt gtgatgcatc tgtatttatg cttaatcaag
     6721 aaggtaaatt aagtggaata aaagatttaa tttattttgg aaatctaaaa agtgcctgta
     6781 aatcagtttt gcatacagga gacaatctaa ctggtgaggg tacaggtgat gatgaacaaa
     6841 tcctagttaa tttggataaa gttccatcaa atattcataa attattgttt gtagtaaata
     6901 tatacaattg tgtagataga aaacaacact ttggaatgat agaaaatgcc tatataagag
     6961 ttgaagacca aggtaataag aaagagatag caaaatacaa tctatctgat aattattctg
     7021 aaaaaactac tcttatagta ggagctatat atagaaaaga tggaagttgg caatttaaag
     7081 caattggtga aggaacaaaa gatgcgggtc ttaaagaagt aatgcaaaat ttagacagga
     7141 ttgagtgtgc atatggaatt taataatgat atcaaatatt ataatgtaga taaagaagaa
     7201 gttacaaaag acttatctgt taatcctgaa aaaggtttat cagaatcaga agtcaagact
     7261 agaagagaaa aatatggttt gaatgaattt actccaaaag aagagggtag tttttgggat
     7321 gatttaaaag aaagtttaag tgaaccaatg atagttatct tgattattgc agcagtagta
     7381 agtgcagtaa ttggggaaac tcatgatgca atagggattg taggtgctat agcaataggt
     7441 atagcaatag gtatgattac agaaggtcgt tctaagaaag cagcagaggc tctttctaag
     7501 ttgactgaaa acatagaagt aaaagtattg agagatgggg aagtacatca aatttctaag
     7561 agtgaattgg taccaggtga tatagtatat attgaaactg gtgacatggt acctgctgat
     7621 ggtagactta tagaaagtat aaaccttaaa attagagaag atatgttaac aggagaatct
     7681 gatgacgtat ctaaaaagtg tgatgtagta gtttctatgg aagagataga atctaaagga
     7741 gcagtagtag tacaggagcc aatacctgca aaacagataa atatggtgtt tggtggtaca
     7801 cttgtagctt atggtagagg agctctagtt gtaacttcaa ttggagataa tagtgaaatg
     7861 ggtaaaattg ctcaaaactt atcagaaaca gatgagcaaa ctccacttca gataaaactt
     7921 ggaaatttag gtggtttgat agccaaagtt tctagtgcag tagcaggatt actatttata
     7981 tttatggttt tccaaatgat aagtaagaat gtattaaatg tagatatgtc tggaatatta
     8041 ccgtttttag aatcaataga cccagttaaa acagcattta cagtatgtgt tgcgttgata
     8101 gttgcagcag tgccagaagg tcttccaact atgataaata tgacacttgc aataacaatg
     8161 caaaaaatgg ctaaaataaa tgcattggtt accaaaaaag aagcttgtga aacgataggt
     8221 tctgtttctg taatatgttc agataaaact ggtacattaa ctgaaaatag aatgactgta
     8281 gaagttgcat atgttgatgg cagatatatt gaaagttcag aagaggaaat aaatagttat
     8341 tttgaagaaa actgtatgat aaactctaca gctgatgtag agcacaatga tggtgatatt
     8401 aagtatttag gaagtgctac tgaatgtgca ttgcttttat attacaaaaa cgtagattat
     8461 agacaagtta gaaaaaattc taatatagta gctcaaaatc cttttacttc agattcaaag
     8521 agaatgactt ctgttatagg tcaagataat catcatgtac tattatcaaa aggagcacca
     8581 gaagtcttac ttgaattatg ttcacatatt caaagaggaa aagacatagt tccattaact
     8641 gaaaatataa aacatgagat tttagaagaa ataaagaaat tgcaaataaa atcaatgaga
     8701 actttagggt ttgcatataa agaaatctca caagctgaag aagaagcagc agtaactgct
     8761 gaaagtgatg ctatgaatgt aagtgcaatg gaaaataatt tagtatttag tggttttgtt
     8821 ggtataagag acccacttag aaaagatgtt atagagtctg taaatacagc aaataaagct
     8881 ggtgtttctg taaaaatgct tacaggagat aatataaata cggcaagagc tataggtgaa
     8941 gagttaggcc tattaaaaaa taatatgaga gtggttgaag cttcatatat agatacttta
     9001 gatgatgaag aattaaaaca agagatacaa agtatttcaa tcgtggcaag aagtaaacca
     9061 gatagtaaaa tgagaatagt ttcagctctg caaaagagcg gggatgttgt tgcagtaaca
     9121 ggtgatggta taaatgatgc tccagcactt tcaaaagcag atgttggtat agcaatgggt
     9181 atatctggta cagaggtatc taaaaacgca gcagacataa ttttaactga tgatagtttt
     9241 agtaccattg taaaaggtat taaatggggt agaggaattt atgataactt ccaaagattc
     9301 gttcaattcc aattaactgt aaatgtaatt gcatttttag tggctataat atctcaagtt
     9361 atgggacaag agatgccatt tactacaata caattattat gggtaaacat aattatggat
     9421 ggaccaccag cactagcatt aggtttagag ccagttagag accatgtgtt aaatagaaaa
     9481 cctgttgata gaaaagccaa tataatttct aagtcaatgg tgtatacaat agtattaaat
     9541 gcattttata taacagcaat actaatgtta caatctacat ttaatttctt aggagttgat
     9601 gaagctcata agggaacagt aatgtttgcg ttatttgctt ttagcgcact gtttaatgca
     9661 tttaactgta gagagtttaa tgcagatagt atcttcccta attttacaca taacaaatta
     9721 gcactacaaa taatagtgtt gacaggaatt gcacaagtaa tatttactca gctattccaa
     9781 gatttcttta actctgtagc aatggatttt atgacatggg taaaagtttt atgtgtagca
     9841 gcaagtgtaa ttgtggttaa tgaagttgta aaatgtataa taagactggt aaaaccaaaa
     9901 aagactgctt aaaacaaatt ttttagaaga aaacagactg actcaaaata tgaattttgg
     9961 gtcagttctg aataaacatt taaattggga ggatagttat gggtgactca aaggtatttg
    10021 aaaaaatatt ctccagccaa tcagatagag gaaattacac tccttcaaag ggatatctaa
    10081 gttattttat ttcgtatatt ggtttagaag atgaagtttt gtacaatctc gagatattta
    10141 agacaaaaca aaatattgat agcaaaaaag atattgcttt atttactgat gttatagcaa
    10201 atcctagtga ttttgatatt ataaattatt ttaaaagtgg acttcaaaaa tatagaacta
    10261 gcatggaaga tgtagatata aatattttgg gatttgaaga gattgattat aaaataaagc
    10321 aagcaatgga tagagtgtta aaagaagagg aaaaagaatt tactaatgat agagttaaac
    10381 aaaattttat agtgaaaata atggcatgga taaaaattta tatcggtgct ttagatataa
    10441 ataaaaatga agctccaaaa gtaatatttt atggagatat aaaaaaacat gaagtttatt
    10501 tactcttaat attatacttg gcaggttttg atgttttata tcttaatcca aattcaaaga
    10561 gtaatataga tattttaaaa tcagaaagat acaatataga gtttgaagaa gccaacataa
    10621 tagaagaaaa aatttctttt gaagaaaggg ttattttagg ggaaaaaata gataaaagtt
    10681 ctgtaaaaaa agcttttact gttggggcag aagcatcaaa gcgtataagt gaggaattgt
    10741 taaatgatgc aggatttata aaaccttggc aactccaaga tagaaaaata aaaaatctac
    10801 ttctaagttc aactgttgat gaaatatcaa tatactggaa tcaaccactt aaattaagac
    10861 caggatttaa gtttaatgat gctattgtag aagctccaaa ttttctttct aagattaatg
    10921 gaatatataa tgataagaat gaatatatta agtttttaga tttattaaga gattcggaaa
    10981 gttcaacatt tattgaattt aatggagatg tagatagatt ttcaaaagct tttacgagag
    11041 aagcttttag tttatccttt ttattggatt caaaaggtgt aatagataaa aactctgttt
    11101 taaataataa agattattcc atatcaacac tagcacttaa tcaacaaatt atgattttag
    11161 agaaagtgga agagttgtta gaaggaagta tgtttttgaa tggtttaagt ggagaagaca
    11221 agataaaagg tctatttaca gtacttcaca tggataagaa atttgttcat atgatgaaca
    11281 atttcgatta ctcacttata aacccaaaat tgattatata catgtacaag agtattgtat
    11341 ttgataaaga aatagttttt cttatgcttt tacttagtaa gattggattt gatattatca
    11401 tattatgtcc aggaggagaa aacaatatag agaatgttat aaataaccaa ttgattgata
    11461 tacacagatt ggataaaatg gtatatgacc ttaaattaaa ttcgcttgaa aatgatatac
    11521 ctttacttaa aaaaattttt ggaaagagga gacgatttta gatggggata aatataaata
    11581 gtttttcaaa agataagaac tctgtagagg ttactgagaa tttgcctaca gcagttcaag
    11641 aagaaaactt cgatataatg gaatatacaa ataataaaaa aaatgaactg agaaagtcta
    11701 aggaagttga ggcattgaca tcccttatag aagtagaaaa tccagatacg attttacagt
    11761 ttggtaggaa agcatctgaa ggagtagcaa gagtttctga tagcctttta aatacaataa
    11821 aattaaatag aaatgaagaa aattctaaaa tgctggttca tttaactaaa ataatggata
    11881 agtttgattt agacgatttt caagaaacta aggaaccaaa tttcgttcaa aaacttttca
    11941 aaaaagctaa taatgccatt gaaatgatgt ttcaaaagta tgaaacattg ggaggagaag
    12001 ttgagaaaat tcaaattgag cttgagcaat acgaaagaga tatagcactt tctaataagc
    12061 aaataggtgc tatgttaaat gaaaactttg agttttataa tgaacttcaa aaatatattg
    12121 ttgctggaga aatggcaata gaagaaatgg ataatgaaat actacctttc tttaaacaaa
    12181 aatcagagac tagtggagac caaatggatg ttgtcaatta tcaagaacta ttaaaagttt
    12241 atgatatgtt aaatcaaaga gtctatgatt taagaatagc agaaaatata gctatccaaa
    12301 caatacctat gcttagaggt atgcaacata ataattatgg tttaattaga aaaataaata
    12361 gtgcatttgt cgtaacttta ccagtattta aacaatgttt atctcaagct atattactta
    12421 aaaagcaaga attacaagca aaatctctaa aagcacttga tgacaaaact aatgaattat
    12481 tgcttagaaa tgcacaaaat gtaagtactc aaagtgcaca aatagctaga atggctggaa
    12541 caagttctgt tcaaatagaa acattggaaa aaatgtacaa tactatcaag tctggtatag
    12601 atgaaactat gagaatagaa gaaaacaata gagctttaat taaggataat acaaagagat
    12661 tggaagaatt aaatacgact ataatttata ataagtagta ttatattaag ggctaatttg
    12721 tataaagttg gccctttcta tgtatatgag gaggctagac attgaaatat ttcgattata
    12781 tctgcaaaga tgatttggag agaatttttt tgaaggagcc agaagacttt agtgctaaga
    12841 ctgaaaaaga tgtgttgaaa tatgcactgg gcgctttttt atacgttcca gctactcaat
    12901 ataatatgat ttataagagt attatagggg atgttaaggg agttagacca ttagctattt
    12961 gtttagaaga tgctgttgga gtaaatggag agttagaggc tatagaaaat cttagattaa
    13021 ttttaaaaaa tattagcaat gaaagtataa ctaataaaga tggaatacca ctaatttttg
    13081 taagaataaa agatgtggag caactgttga gaattaaaga gattataatt aaaaatagtc
    13141 attctataac aggaatatta attcctaaag caaattcgga gttgattgaa aattgtatag
    13201 aagctttata ttctatgaat cttcaagata tgtatgttat tccaataata gagacaagag
    13261 aatttatata taatgaaaaa aaagaactta gttttactaa tttgtacaat gctattttaa
    13321 gacataagtc aagaatatta agcattagag taggcataac tgatatatta gggatgtatg
    13381 gtataaggag agataaaaac ttctctatat ataataattt gatttgttct tcatttatat
    13441 tagatataat cacatatctt caaagaccag agttagatat acctataagt ggtggagtat
    13501 cagaattttt tgatatgaca aataaggata taagaaataa gtatatagaa gaaatattat
    13561 tggataaata tcatggatta attggaaaga ccgtaattca tccaatgcaa attcaaattg
    13621 tacaagcgct atctacagta tcatatgaag attttactga tgctttggat atactggata
    13681 gtacagacag taagtatggt gtgagcaagg gtgtattagg agagagaatg aatgaaacaa
    13741 atccacattt tttatgggct aaaaaaacat tgattctatc taaaatatat ggtgtattaa
    13801 ataaaggagt agactatgaa gaattgctta aattttaatt gtgagattga cattattgat
    13861 aatccattga atttagaact aaatgatttt ttagacgtag gagtaagaaa taatagcaaa
    13921 agacgttttt tgtttatctc aaagaaactc ggtaaacatt tagcatgtaa gccaagtgag
    13981 atggataatt tgggaaaatt aatggctaca atttataatg aaaaaaataa ggaagaatca
    14041 aatggtactg ttatagcttt tgctgaaact ggtacagctg ttgggcatag tttttttgat
    14101 tacttatgtg gagattatga atttattcat actactagag agcagtttga cgagttagaa
    14161 aatctggaat ttttagagga acattcccat gcaacagacc agaatttata ctttggtatg
    14221 cttaacaact ttaaatttgg agatgagata atattggttg atgatgagat taccacagcc
    14281 aatacatgca tgaatataat taggaaaatt caaagcattt atcctaaaaa gaaatacact
    14341 atatgctcaa tactcaattg ggtaagtgat gaaaatttaa gtaaagttac tcgtttagaa
    14401 agagagctaa attgtaagat agattttgta tacttattta aaggaaactt taaattttat
    14461 atagatgaag atattatttc aaattacaac gataattata gtattactaa attagagact
    14521 atttcaagtg ataaagtaaa tttagtagta aatcatattt atgttgattt agaagattat
    14581 attggaaata agaaatatgt aaaatataca ggaagatttg gaattaatag aagagaacaa
    14641 tttagattaa aagaaatcgt taagagagaa agtaaaaaaa taactatggc cagtaaagaa
    14701 tctttaaaaa atgaaaaaga aaagattcta tttttaggta ctgaagaatt tatgtatata
    14761 cctatgctgt ttgcaaaaga aaaagaaaat caatatgata tatactatca ttcaacaaca
    14821 agaagtccaa ttattaatat tgataagaag cattatccta ttaaaagtaa atttatgcta
    14881 aagagttttt ataatataga tattcaaaat tatatttaca atatagataa acataatttt
    14941 tctaagtgtt atttatttgt agagctgaat aaaagtgaaa attcttatct agaatttatt
    15001 gatataatta aaaaaacaag tataaaagaa ttaactatag tatgttgtag ctaaagtgtt
    15061 ttaacaggat aaaatatatc acgattttaa gataggagtt gagtcatatg agagatttta
    15121 gcacgtattc taaagatgat gttatttttt tattaaaaga tatatcaaat ttaattgaag
    15181 aagaaggaaa taaagaaaga gaattaaaaa tacaaaatgg tagacattat tcagaaatga
    15241 taccaataga gtatgaagtt agtgaagatt atctgaattt atatcatgag aaactaagag
    15301 aaaataaaga taagcttgca ttttctatag gagttatgtg tgaaaaaata attaaaaaaa
    15361 atggaaatga tgtagtacta gtttcacttg ctagagcagg aacacctatt ggtattttgg
    15421 caaaaagata tataagaagc aagtataatg taagtttacc tcactataca atctctataa
    15481 taagagataa gggaatagat atgaatgcaa ttgaatatat agtaaagaat catccgagct
    15541 caaaaattca atttttagat ggatggactg gtaagggaac tatatcaaaa gaacttgaaa
    15601 aagcatgtga tgaacttaat gttaaattta aaaaacattt tgactcaaca cttgctgtat
    15661 tagctgaccc tgctggatat tcaggacttt atggaattag agaagatttc ttaataccaa
    15721 gtgcatgttt aaattcaacc gtttctggtt tagtaagtag aactgtacta agagaggatt
    15781 taattggtaa aaatgacttt catggagcta agttttatag acatttaaaa gataaagatg
    15841 aatctatgaa ctatatcgaa actatagaag aatgttttaa aaatcaattt aagaatatat
    15901 ctgatgaagt tgaaaactgg gaaagtgaca ttataacaag agatggatac tttgatgttt
    15961 taaatataaa agaaaagtat aacataacag atattaactt tatcaaacca ggtgtagggg
    16021 aaacgacaag ggttttactt agaagagttc cgtataagat tcttgttaaa gacttaaatg
    16081 ataaatcttt agaccatata ttcatattag ctaaggagaa aaatgttgaa gttgaacaaa
    16141 tggatttaaa agcctataaa tgttgtggaa taataaaaaa tatgaaagat atttaggaaa
    16201 tatattagac aggagaatat tgaatgattg ttttttcaga tttagacagg agtatcattt
    16261 actccaataa atttttaaat gctgatagta aatatgctaa tatagaaatt tatagggaaa
    16321 aagaaatatc ttatatatct ttagatacta taaatttaat aaagcaaata caatattatg
    16381 gtatgtttat acctacaact actagaacag tggagcaatt taagcgtata gaatttaata
    16441 aatatgggat ttattttcct tggagtataa cttcaaatgg aggagttata cttaaagata
    16501 atgaaatatt gaaatcatgg agtgaaaaaa tagataaact aaaacctgat tatgagccca
    16561 ttgaaagtat gattcataaa tttaaagact atttgaatgt agatggaata actaatttta
    16621 aagttgcaga agatacattt ttttatatag ttgttgattt ctcaagattt aatttggatt
    16681 caatcaaaga atatacaaat atattggaat caaaaaattg gaagttttat gtgagtggca
    16741 gaaaaatata ttttatacca aaagaaattt caaaagaaaa tgctataaaa tacttgacta
    16801 aagaattagg aatagaatat ttttatgcag ttggagattc tattatggac tatggtatgc
    16861 ttaatatctc aaataaatcc tatgtattga agcatggtga tataaataaa aatgaaatag
    16921 aaaattcatt tatatcaagt tctttcaaag gaatgagtgg aactgaagag atattatcta
    16981 acattttaga cgagaactgc ctacttaata tataatgact gatatataaa taataaacta
    17041 gaaaaatctg aaataaatta tagtaaagtt tatcatacta atttatttca gatttttatc
    17101 aatttatatt taagtaagaa ctcaatatgt taaaatgaag gtggagtcat tgcccagact
    17161 gagatacata cctcatcatc ataattttca tacacatgtg gtatagtact gttaaaataa
    17221 aaactatctc cttcctccaa tagatattcc ttatttccaa gtgttatctt cattttacct
    17281 tttattatat agccacattc ttctccttcg tgtgtcacaa attcactgtt tttttgtgta
    17341 tgaccatcta gcgtaacaag tataaattct atttttttcc cagataaatt aggaacaaga
    17401 agttcataaa attttgtaga atcattagtt actatttttt ttcgattatt tttctttact
    17461 actatatcag agtcctctct tccaatctct tcaaaaaaat aacctatatt tatatcaaga
    17521 gcttttgata ttttccacat tgctacaata gatggaacaa ccatattacg ctcaatttga
    17581 cttattaagc ctgtacttaa atctgaattt tttgctaaag tagagatact tatattttta
    17641 gattttctaa gagatctaat tttgcttccg acatctatat tcattagata caaaccccct
    17701 cagacatgat tataacatat aatttataaa gattaataaa ataaaaaaaa gttttataat
    17761 gcatgtattt tttatgttat attgaatttt ctgaaaagat gcattataat agtattaata
    17821 ttcaatatat tgaatattga taatgatatt ttaatttaat gaaatggagg taattaagat
    17881 gaagagtttt gtaagtcctg ataatatttg ggtattatgg gcagttgtaa ctggatgggc
    17941 agcatttagt atttacttgg aacaaaagta caattgggca tcaaaagtgt cgggagcaat
    18001 aatagctctt gtaggagcaa tgttattatc aaatctaaat attataccag tagagtctgt
    18061 tgtatatgac caagtatggg gatatgttgt tcctttagcc atagctttat tgttatatca
    18121 atgtaatatt aaaaagatat ggaaagaaag tggaagattg ctcattatat ttcttgtagg
    18181 ttctgtagga actgtacttg gagctatgat tggattttta gcattaaaaa atgtagtacc
    18241 agatttaaat attgttgcag ctatgatgac aggttcttat ataggtggaa atgtaaactt
    18301 tgctgctatg tcaggggcgt ttgatgcgcc aggagaatta gtatcagcaa cagttgtagc
    18361 agataatctt ttaatggcat tatatttctt tgtcttaata gctataccat caattggatt
    18421 ttttagaaaa cattttaaac atccacatgt agatgaaatg gaaagtatag gtataactga
    18481 aggagaaaca gtagcagcaa attattgggg aagaaaagaa atatctttaa aagatatagc
    18541 aatagccatt ggtagcgcct ttataattgt cgcagtatct gtagaactat caacttggtt
    18601 taaagctata ataccattat caaatccttt tttagcaatg ttaaatactt tatttggaaa
    18661 tcaatatcta ataataacta ctttgactat gttgtttgcc acatttatgc ctaatttctt
    18721 tggtgatata aaaggagcac aggaattggg tacattttta atatatatat tctttgttgt
    18781 agtaggtgtt cctgcttcaa taacattaat aattcaaaaa tcaccattac tacttttata
    18841 ctgcggaata atggtactaa taaacatgct agttacattt atagttgcaa aaatatttaa
    18901 gtttagttta gaagaagcta ttcttgcatc taatgcaaat attggtggtc ctacaacagc
    18961 agcagctatg gcaatatcaa aaggatggtc taagctagta gggccaatac ttatagttgg
    19021 aacatttgga tacatagttg gtaattattt tggtctttta gtaggaaata ttttgatata
    19081 attttacgtg aaaagaagtt gttttttaga aagattttta tttaattgga ggttgttatg
    19141 attttcgatg gacattcaga tacttgggca gatgtaacaa taagaaggct taacggggaa
    19201 aaagatgtat ttagaagaat ccatcttgat aattttaaaa gagcaaatat agaaggagct
    19261 atttttgtaa tatggataga cccaccatat gataaagagc ctgaaaaaag agttaaacaa
    19321 attataagct ctataaaaga tgaaattgaa gatatgaaag atgttgtaaa catagtaaaa
    19381 aaatattcag atatagataa aggtataagt gaaaacaaaa taaatatttt aattggtctt
    19441 gaaggattaa gccatattgg aagaaatata gatttactag attattttta taaagaagtt
    19501 ggtgcaagac atgcatcatt aacttggaat gaacagaatg aacttgctac aggatgttta
    19561 ggggatgaaa atcgtggttt aactgaaata ggtaaaaaag cagtcaataa aatggaaaaa
    19621 ttgggcatga tagtagatgt gtcacattta aatgaaaaat cattttggga tttaatgtct
    19681 gttgcaagta agccagttat agcttctcat tctaatgcta ggagtttatg tgatgctaaa
    19741 agaaatctta cagataatca attaaaagaa atttcaaata caggtggatt agttggaata
    19801 aatagtttta gaggatttac taatagtaat cctaagaagc aagatttaat acatttggtt
    19861 gaccatatag actatatggt aaatcttata ggtatagaac atgtatcttt tggttttgat
    19921 tttagtgatt acttagggaa agagactata ggttctttta ctggaaatgc tagtgtgcca
    19981 agtgtagatg gtcttgaaag cacacataca gcacagaaca tagtagtaga gcttgaaaat
    20041 agaggttata aaaagaaaga tatagagtta atctctagtg gtaattttta taggctcttt
    20101 aaagatatac taaaataata taataaagaa cttaaattta taaaatagta tatttataca
    20161 ataatatcta catatttaaa ttggcgttca taatttatta aattaaattt taatgttact
    20221 agaagtttca attctagtaa cattttttat gttaaaaata gatatagata tatattgaca
    20281 atgataatta ttattgataa actataaaag tgaaaatgat aataattttc attttaatta
    20341 ataaaagatt tttaaggagg gagaatgaag aaaaggtatt tagtcatagg aataataatt
    20401 ttgtcttatt tttctttatt tataggagct gaagatataa atataatgca tatatttgca
    20461 aaaaatcagc ataagcttat gatatttatc atgagtaggg taccaagatt aataagtata
    20521 ttgattgctg gtgtaggaat gagcatagca ggtcttataa tgcaacagat aagtaagaat
    20581 aaatttgtat cacctacaac aggtgctaca atagatgcag ctcaatttgg gatagtaata
    20641 tgtatgcttt tagtaccaac tgcctctatt tttacaaaga caataatagc atttgtattt
    20701 tctcttgtag ggacttttac gtttatgaaa ataataggga agttacaatt taaaaatata
    20761 atctttgtac cattagtagg tataatgttt ggaaatataa taggttctat gacggatttt
    20821 atcgcgtata aatacgattt gagtcaaaat gttagttctt ggatgcaagg tgacttttct
    20881 atgatactta aaggaaatta tgagatatta tatatcacaa taccccttat aatattagca
    20941 tatatttatg caaataaatt tactgttgtt ggtatgggta tggattttgc gacaaactta
    21001 ggtcttagtt acaaaagaat tgtcaatata ggtctgataa tagtagcact tgttacagta
    21061 tgtgtagtcg ttacagctgg taatatccct tttataggtc taatagttcc caatatcgta
    21121 tccttgtata tgggtgataa tataagagca agcatatggt atactggttt acttggagcg
    21181 gtatttgtac ttatatgtga tatttttgga agaattatta tttatccata tgaaatatct
    21241 ataggactta cagttggagt tataggaagt atactattcc tttatttaat attaaggagg
    21301 aatgtgaatg aagcttagtc tcaatcaaaa aatttacttt gtaagtatat tactattcat
    21361 atcaatagca ctttttataa tttatggaat agacatgaat catttagaat atgcattatc
    21421 acaaagaata ccaaaaatat ttgctatggt attaggtgga ggatgtatag catttactac
    21481 tgtagtgttt caaacaatta caaacaatca aatattaaca ccaagtgtac taggtttaga
    21541 ttcattgtat gtaatgattc aaactataat agtattcata tttggttcaa gcagtagtct
    21601 gattataaat gaaaattata attttattat taatgtagct atgatgattg gagcttcatt
    21661 gttattatat aagatgttat ttgaaaaaaa taaaaataat attttctttt taatcctagt
    21721 aggtatgata tttggaacct tatttaaaag tgcaacaaca tttattcaag taatgataga
    21781 cccgaatgaa tttttagctt tacaaactag tataatggca agtttaaaca atataaacac
    21841 taatgtattg ttaatagcat ttataataat tattgccata atacctttta tatatgatga
    21901 aataaagtat ttagatgtat tgtcgttagg taaagaacaa gcaattaatt taggtgtaga
    21961 ttttgataag gcagttaaaa aaatgataat attaatagca attttagtct caatatcaac
    22021 agcacttatt ggacctatga catttttagg acttttactt gctaatataa caagagaaat
    22081 ttttaaaaca tataagcata catacttgat ttcgggttct atgttgatag ggatgattac
    22141 attgataata ggtcaatttt ttatacaaca tgtatttaag tttgatacga ctttaagtgt
    22201 agtaataaat tttataggag gaatttactt tattcaatta ctattaaagg gggcaaatag
    22261 atgatagaga taaaaaatat atttaaaaga tataagaata aaaatgttgt agatgatgtc
    22321 tcttttagta tagaaaaggg aaagataaca tcttttatag gaccaaatgg agctggaaaa
    22381 agtacagtac tatcaatagt tacaaggctt ataggaggtg atggcggaga agtaataata
    22441 gaaggaaaaa gcttgactaa ttatagcaat aaagaattag caaaaaaaat agctattctt
    22501 aaacaatcta acaatataac tttaaagctt acaataagag aattagttgg atttggaaga
    22561 tttccatata gtgaaggtaa tctaactaaa gaggatgaaa attatattga tgaagcaata
    22621 gaatatatga agttgactga tatacaacat aagtatttag atgaattgag tggaggtcaa
    22681 agacaaagag cttatatagc aatggtaata gctcaagata cagagtacat attacttgat
    22741 gaaccactga acaatttgga tatgaaccat tccgtacaga tgatgaaggt acttagaagt
    22801 ctttgtgatg agttagataa gactatagta ttagtaatgc atgatataaa ttttgcatca
    22861 tgttattcag ataatatagt tgcattaaaa aatggaaaag ttgaaaaagt aggtcgtaca
    22921 gatgagatag taaatgaaaa agttttagaa gatatttatg agatgaattt taacattaag
    22981 aatataaatg gaaatcgaat ctgtatttat ttttaaaata ttgtatatga aaataagctt
    23041 gctatatgat tttttataaa gtttttaaat taaaataaag attaatagga gagaggaaaa
    23101 tattgatatg aataaaaaag cagcaatagt agcagcagta gctattatag gattagtgac
    23161 agtgtttgca ttaggtggaa gtaaaaagaa tgaatcaaaa acatcagaaa attcaaataa
    23221 taccataaag ataactcata acttgggaga aacagatgta aaattaaatc ctaaaaaagt
    23281 agtagtattt gattattcgg ctttagatac aatggatgca ttaggtgtag ctgagaattt
    23341 agtaggactt ccaaaaacga gtttaccagc atctttagaa aaatataagg ataagaagta
    23401 tgcagattta ggaggtttaa aagaaccaga tttagaaggt ataaagtcgg caaatccaga
    23461 tttaattata ataaatggaa gacaagaaga tttttatgaa caattgtcaa aaatagcacc
    23521 aacaataagt acaagtaaag atgataagaa gtacttagaa tctgttaaaa ataatataga
    23581 caaaatagca aaaatatttg gagtagaaga aaaagcaaat caagaattta gtaaaattga
    23641 aaagaaaata gaaactttga ataaaaaagt aacagataaa aatttaaatg ctttaactat
    23701 aatggtaaat gaaggtaatt taagtgtatt tggtgaagaa tcaagattta gtatattata
    23761 caatagtttt ggatttgaaa ataaagataa aaatataaaa gaatcaagtc atggacaaaa
    23821 tattactttt gaatatatag ctaaacaaaa tccagaagtt atgttcgtta tagatagagg
    23881 aatagctact ggtagtgatg taaaagaaag ctcaactgca aaatctgttt tgaacaatga
    23941 tataataaaa tctatggatg catataagaa tgataatata atatatttag attcaccaac
    24001 atggtatgta aatgatggtg gtctaacatc tttaaataaa atgatagatg atgcttcaaa
    24061 agcggtaaat taataatccg atataatcaa aatgtatttt ttgtaaaatg attatcaaat
    24121 aaactaggag tttttaagaa ctcctagttt attttttgca taataaagat atagtttaaa
    24181 caataatatt atttataata agataaatct attcaaaatc taataaaata tgaagttata
    24241 ctagacaaaa agaataactc ttaaatataa
//
LOCUS       DAGQWR010000059        24268 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.059, whole genome shotgun sequence.
ACCESSION   DAGQWR010000059 DAGQWR010000000
VERSION     DAGQWR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.059"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            109..300
                     /locus_tag="MIW95_001977"
     CDS             109..300
                     /locus_tag="MIW95_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBY3151304.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            455..1864
                     /locus_tag="MIW95_001978"
     CDS             455..1864
                     /locus_tag="MIW95_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY3151305.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIRIFKDKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKICGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            1995..2684
                     /locus_tag="MIW95_001979"
     CDS             1995..2684
                     /locus_tag="MIW95_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBY3151306.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2687..3622
                     /locus_tag="MIW95_001980"
     CDS             2687..3622
                     /locus_tag="MIW95_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBY3151307.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3619..4512
                     /locus_tag="MIW95_001981"
     CDS             3619..4512
                     /locus_tag="MIW95_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBY3151308.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4590..5336
                     /locus_tag="MIW95_001982"
     CDS             4590..5336
                     /locus_tag="MIW95_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBY3151309.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRASSRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5337..6170
                     /gene="rsmI"
                     /locus_tag="MIW95_001983"
     CDS             5337..6170
                     /gene="rsmI"
                     /locus_tag="MIW95_001983"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBY3151310.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6193..6897
                     /locus_tag="MIW95_001984"
     CDS             6193..6897
                     /locus_tag="MIW95_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBY3151311.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7085..7513)
                     /locus_tag="MIW95_001985"
     CDS             complement(7085..7513)
                     /locus_tag="MIW95_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBY3151312.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7550..8980)
                     /locus_tag="MIW95_001986"
     CDS             complement(7550..8980)
                     /locus_tag="MIW95_001986"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBY3151313.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9112..9696
                     /locus_tag="MIW95_001987"
     CDS             9112..9696
                     /locus_tag="MIW95_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBY3151314.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9712..10233
                     /locus_tag="MIW95_001988"
     CDS             9712..10233
                     /locus_tag="MIW95_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBY3151315.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10299..10545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10629..12566
                     /gene="metG"
                     /locus_tag="MIW95_001989"
     CDS             10629..12566
                     /gene="metG"
                     /locus_tag="MIW95_001989"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBY3151316.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12596..13366
                     /locus_tag="MIW95_001990"
     CDS             12596..13366
                     /locus_tag="MIW95_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBY3151317.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGICYEEVCRVTKENAKKFFNIK"
     gene            13703..14134
                     /gene="phnG"
                     /locus_tag="MIW95_001991"
     CDS             13703..14134
                     /gene="phnG"
                     /locus_tag="MIW95_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBY3151318.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14149..14724
                     /gene="phnH"
                     /locus_tag="MIW95_001992"
     CDS             14149..14724
                     /gene="phnH"
                     /locus_tag="MIW95_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBY3151319.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTVSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14727..15812
                     /locus_tag="MIW95_001993"
     CDS             14727..15812
                     /locus_tag="MIW95_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBY3151320.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15832..16701
                     /locus_tag="MIW95_001994"
     CDS             15832..16701
                     /locus_tag="MIW95_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBY3151321.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16694..17560
                     /locus_tag="MIW95_001995"
     CDS             16694..17560
                     /locus_tag="MIW95_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151322.1"
                     /translation="MSKINVSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17601..18314
                     /locus_tag="MIW95_001996"
     CDS             17601..18314
                     /locus_tag="MIW95_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY3151323.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18315..19484
                     /locus_tag="MIW95_001997"
     CDS             18315..19484
                     /locus_tag="MIW95_001997"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBY3151324.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDRIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVLNTIVNGKTVYKREV"
     gene            19488..20324
                     /locus_tag="MIW95_001998"
     CDS             19488..20324
                     /locus_tag="MIW95_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBY3151325.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20325..21320
                     /locus_tag="MIW95_001999"
     CDS             20325..21320
                     /locus_tag="MIW95_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY3151326.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLDVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21338..23026
                     /locus_tag="MIW95_002000"
     CDS             21338..23026
                     /locus_tag="MIW95_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBY3151327.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYIIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23038..24105
                     /locus_tag="MIW95_002001"
     CDS             23038..24105
                     /locus_tag="MIW95_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY3151328.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9683 a         2707 c         4339 g         7539 t
ORIGIN      
        1 ttttttgtca aaaaaaacaa aaaatacgta tttatttttt catttcggga aaactaaaca
       61 caggaattat ggactttata aaaatttaat tcttgaggag tgatataagt gaaaaaagtt
      121 gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat tctaggtaca
      181 gacttaatag gtagtatctt tggtggaaca tatgaaatgg taagtagaat aatatacttc
      241 attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa
      301 tttatgcttt tatgttaaat acctcattct aaatgtttag aatgaggtat ttaattttat
      361 aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa gttttaaaaa
      421 gaagtattat gtaaaaaagg agtactatta atatatgttt attattaatg aattaaataa
      481 gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaaagg gacaggttta
      541 taaaaagtta ggatataaag atatactaaa aaatttgtat aaaatggata caactgagat
      601 attaggaaca gataatttac actttccgga aggaattata aaaaattcac aaaaaaaagc
      661 atcaagagta tttaaaagtg attatacttg ctatttagta aatggtagta gctgtggaat
      721 acaatctgca ataatgtcag tatgtagtcc taaaagtaaa ataataacaa atagagactg
      781 tcatcagtct gttataaatg gatgtatttt aggagatata gatatagagt atgtgccttg
      841 tgagataagt gaagagacaa atgtcttaaa agggatatct ataagtaatg tgattgatat
      901 aatagataaa aacttggatg caaaagcagt actgcttact tatcctacat attatggaat
      961 gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaagatgg tagtgatagt
     1021 agatgaagca catggtgcgc atttagggct tagtgagagg cttcctaaaa cagctcttga
     1081 gcaaggtgca gatatagttg tacagagtac acataaaact cttccagcat ttacacaatc
     1141 gtcaatgata catatacagg gagaccgagt tgatatagat aaaatattga acatgttaag
     1201 aattacagaa tcttcaagcc cttcatattt attattatct tctctggaat tagcagtaga
     1261 tatatatgaa aataaaggaa aagaattaat ggaagagctt ttaaataata ttagaatttt
     1321 caaagataaa gttgattcaa atattaatat atatgacaca aatgataaaa caaaaatatt
     1381 tatatcatct aaaaacatgg gtttaacagg ttatgaacta gaaaatattt tgagaaacaa
     1441 atataacata caagtggagt tatctaatta ttatggagtt cttttaatat gtacaatagg
     1501 aaatactgct gaggatttct ctagtttaga aacagcatta aacaatgttg taaaggaaga
     1561 ctttaaattt gaaaagttag aaaataaaaa ataccctgtt gttattccaa agaaaatatt
     1621 aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg acagcatagg
     1681 taaaatttgt ggtgaatgta tagttccata cccacctgga ataagtgtaa tatctcctgg
     1741 tgaagtaata agtaaagaaa taatagacta tctaatgttt tgtcattcta aaggcatgat
     1801 tgtgagtgga ttgaaagatg tagatttaaa ttttatacaa gtaatagatt tagaagacat
     1861 ttaaaaatta tgatataaaa gtagatagaa gtagatagtt taaaattgtg aagagaaata
     1921 aatagactgc ttatatagag ctatctaata aaagtactaa aaaagtaaaa agtgtttgta
     1981 tggaggatat gaaaatgaaa ggtaagttaa taattataga gagtggttca gatgcaagtg
     2041 gaaaggctac tcagacagaa atattatatg aaagattaaa aaaagaaggc tatatgatta
     2101 aaaaggtaga atatccaaat tatgaatctc catcatctgc cttagtgaaa atgtacctaa
     2161 aaggagactt tggtaaaaat gcatcagatg tagacccata tatagcatca acttttttta
     2221 cagctgatag gtatgcatca tttaaaaccg attgggagga gttttacaat gaaggtggaa
     2281 taattatcgc agatagatat acaacctcta atatggtaca tcaggcctct aaaatggata
     2341 tttgtgatag agaagagtat ttaaattggt tagctgatta tgaatttaat ttatttaaga
     2401 taccacaacc tgattgtgtt atatttttag atgtgcctat tgaatttagt aaaaagctta
     2461 tggagaatag aaaaaataaa attacagggg aagaaaaaaa ggatattcat gaaagtgaca
     2521 tagagtattt agagaaatct tacaacaatg ctttatatat agcagataaa tatgattgga
     2581 aaaaaataaa ttgtgtttta gataacaaat taagaagtat tgaaagtata cataatgaaa
     2641 tttataaaat agttcttgat tcaataaaac ctatggagag ataaatatgt attttgaaaa
     2701 tattataggg caagattttg caaagaaata tttaacaaat tctataaaga aaaataaact
     2761 taataatgcg tatatgtttg aaggaatgga tggaatagga aaaaagaaat ttgcagatga
     2821 attatcaaaa ctattacttg attatgaaaa tctcgaaaat agtccagact atgtactcat
     2881 aaaaccagat ggaaatagta taaaaatagc tcaaatcaga aatttacaat cagatatagt
     2941 aataagacct cataaagact ataaaatata tataatcaat aatgctgaaa aaatgactgt
     3001 ggaagctcaa aatgcacttt taaagacgct agaggaacct ccaaactatg ctataataat
     3061 cttagtcaca aacaacaagg aatctttatt agaaactata aaatctagat gtgatataat
     3121 aaagttctca cctataccaa ttgaagactt aaaacgttat ttgataaata caggtataga
     3181 agaagaaaga gctcaattat tggctatttt ttcgagagga agtattgaga atgctctaaa
     3241 tctatctcag tcttctgagt tttcaatgat gagagaagat atacaacagt atattcagat
     3301 aatgttagat aaaaacatag tggagatttt aaatatacca aacaatatgg aaaaatatag
     3361 aggtaagatt atagctttat tagatatgat gataaactat tttcgagata tcatcctatt
     3421 aaaagaaaat gtaaacaaga acatgttaat aaatgtagat aagctagttt tcattcaaaa
     3481 tatgagtgga aaaattagtt attctcaatt gtccaagatt attgatataa tagaagatgc
     3541 aaagagtaaa ataaaaagca attgcaattt caatataagc atacaagtta tgtctttaaa
     3601 tatatacgag gtgattaaat gataaagata gtaggtataa ggtttaaaag tgctgggaaa
     3661 atatattatt ttgaccctgt tgattttaat atagagcaag atatggatgt agttgtagaa
     3721 actgcaagag gcttggagta tgggaaagta gtcgttggtc caaaagatat ggatgaaagc
     3781 gaattagttt ctcctttgaa gccaattata agaatagcta cagaagaaga taaaaaagta
     3841 tatctagaaa ataaggaaaa ggcaaaagaa acttttgagt tgtgccaaca aaaaataaaa
     3901 gaacatgagt taacaatgtt tttaatagat tgtgagtata cgtttgatag gaataagttg
     3961 attttttact ttacagcaga aggaagaata gattttagag aattggtaaa agacttagct
     4021 gctatattta aaactagaat agaacttaga caaataggtg tcagagatga agctaaatct
     4081 ataggaggtc tagggccttg tggtagaaaa ctttgttgtt catcttggtt aggtgatttt
     4141 caaccagttt caataaaaat ggcaaaagac caaagtctat ctctaaatcc aacaaaaata
     4201 tctggaatat gtgggagatt attctgttgc ttaaaatatg agcatgatgt atatagtgaa
     4261 gcaatagaaa aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg aaaaggaaaa
     4321 gttgtagaag tgaatccttt attagaacaa attaaagtgg aatttcaaga caagacagta
     4381 aagatataca caagagaaga tataaaaata cttagagagc ctaaaaaatg tgatggctgt
     4441 ggtggtaaat gtaaggataa tgatggatta gatgaagcca cactaagaga gctaaagaaa
     4501 ttagaagatt aaatttaagg tagggctttt cctacctttt ttaaagcaat taatgcttaa
     4561 agttattaat atttgaggag aatttatata tgcaattaaa agattcagaa agaatagatg
     4621 atttacaatt aaaaggcctt aaaattatac aagatactaa tggtttttgt tttggtatag
     4681 atgcagtttt attagccaat tttactaaaa taaaaaaaga tgccaaagta gtagatttgg
     4741 gtactggaac tggaattata cctattttga tagctggaaa aagtgaagct aaaaaaatta
     4801 ttggcgtgga aatacaagaa gatgtatatg aaatggcaac tcgttctata aaattaaatg
     4861 gattagaaga aagagttgag attataaatg aagatataaa atacatagac aaagtcttag
     4921 acataaatgg ttaccatgta gtcacatcaa atcctccata tatgcatatt gatggaataa
     4981 aaaatccaaa tgataaaaaa gcaatatcaa gacatgaagt aaggtgcaat ttggaagatg
     5041 taattagagc ttcatccaga ttggttatgc ctagaggaaa attcttcatg atacatagac
     5101 caacaagatt ggttgatata ataactttag ggagaaaata taaattagaa ccaaaggtta
     5161 ttcaatttgt gcatccaaga cctcaaaaag caccgaactt agttttggtt caatttgtaa
     5221 aagatggaag accagagctt aaaatattgg acccattgta tgtttatgga gaagatggga
     5281 attatacaaa agaattaaaa gcaatatata ataataaaga tataggagag ttgtaaatga
     5341 gtgggaaatt gtacatatgt ccaacaccaa taggaaacct agaagatata acatatagaa
     5401 ctttaagaac attaaaagaa gtagacttga ttgcagcaga agatacaaga catagtataa
     5461 aacttttaaa tcattttgaa atctcaaaac cactaactag ttatcatgaa cataataagg
     5521 attcaaaggg agattatttg ataaataaac taattgatgg tgaaaatata gctctgataa
     5581 gtgatgcagg tatgcctggt atatctgacc caggagaaga gataataaaa caagctatac
     5641 aaaataacat agagatagaa gtattgccag gtgcaacagc ctttgtaacg gctttagttg
     5701 gctctggtat ggacacacat agatttgttt ttgagggctt tttagataga gataaaaaag
     5761 ttaggaaagc acaattggaa gaattaaagg aagaaagtag aactataatt ttctatgaat
     5821 caccacatag attgaaggat acactaaaag atatgcttaa aattcttggt aataggaata
     5881 tttctataaa tagagaactt acaaaaaaat atcaagaaat aataagagaa aatatagaaa
     5941 ctaccataaa aatatttgag gaaaaagaag taagaggaga gtttgtatta atagtagagg
     6001 gttttcatgg tgagaagtca aagaaagaaa attatgatgg gcttacagat agggaatatg
     6061 ttttaaaact aattgaaagt ggtatgaaca aaaaagatgc aataaaaata gtttgtaaag
     6121 atagaaagtt aaagaaagat gtggtttata aacaagtttt agatttataa aacctgaatc
     6181 tgaggtgatt ttatggaaat agggaaaatt ataatagaga gtttaaagag ggaagataca
     6241 cataaaatat ttatagaatt agataataag catgaattag aaaaaataat tcctaaaata
     6301 agctgtatga agtctgtagg tgaatgtaaa tatcatgtag taaattgttt tgaacattct
     6361 ataaatgcat taaaagaatt agaaatagta ttaaatgata aggatttttt tccaatacat
     6421 ctaagaaaac atgtgtcaaa ctatttaaac acatatattg aagaaggaat aaataagtta
     6481 catgtattaa agttgggaac atttttacat gatataggta agcctgatag tatgacctta
     6541 gatgaaaccg gaagagttca tttcacaaat catgaaaaaa taggtgctca aataattgac
     6601 aatatgggaa taaagttaga gttatcaaca gagacatata gacttataag taaatatgta
     6661 agatatcata tgatactttt gtctctatat aagaaaaatg atttgagtag gaaagaatta
     6721 attaatgtat ttaacttagt agatgaggac actataggtg taataatgct tgggtatgca
     6781 gatatagtgt ctacagtaaa attattgggc aagagtggag aagtcagtgt gttaaagaca
     6841 tatatggaat atattttaac aaattacttg tataagagta attatacaca tgtataaatt
     6901 tagttaatat aattgagttt tagatgatta attgaagttt gaaatatacg gatttcaaaa
     6961 acataaaaga agttgtcttt gttgtaaaat tatgaaagac aacttttttt atatcaaaaa
     7021 ttgaaatttt attaaggata cttaatggtc tattaattaa taaaccatta gtatttaatt
     7081 tatattacaa tattgatttt gcttgtattg gcgttgatag tataacagag gtcttagtag
     7141 aaccaatttt ctttatagta tcaatcacat tttctagctc gtacatatct tttacaatta
     7201 cttttaaaag taagcaatca tcacctgtta tatggtgaca ttctacaatc ctagggtcct
     7261 ttgcagctga ctcaataaat tctgtatatc cattgcttgg aagagaaata tgaataaatg
     7321 cctttataac tctgcctaat gaatctgggt tgacaatagc tttatatcct tctataacac
     7381 cagactcttc taatcttttg actctttctg aaactgcagg agaagttaaa ccaactattt
     7441 ttcctaagtc cttcatagaa attctcccat catcctgtaa aatttctatg attctgtaat
     7501 ctgtaacatc cataaataaa cagtccccct taaataaaaa taattaatac tataaaactt
     7561 ttttagaatc tatcatttgt gtttttaaat accaaagatt tctagataaa tcaaccttat
     7621 aaatgtgtgg ttttgaaagt cttttgtatt gttcccagat agtacttaat tcatattgta
     7681 catgattttt tatttcaaga acagcttttt tattatattt gcatctgcct tttatataca
     7741 atggtttaag aagctcttta actttgtaat ttgtaaatac ctttgttttc catgtataag
     7801 taggatggaa gatttctaaa ggtttagact catctataac ttcatcatgt aacataatca
     7861 aatctgcctc tgccttattg tcttcgttat atattcttat aactttcttg taaccaggat
     7921 tatttatttt ttctggattc tcagaaatct ttatcttagg ttcaagaact cctttttcac
     7981 aagatgcagc taatttataa actcctccaa gagaaggtga atcgaaagat gtaatgagct
     8041 ttgtacctac tccccatgaa tttatagtag ctccttctgc tttaagtgat gttatggtat
     8101 attcgtctag gtcattagat gcagtgatgc tgatatctga aaagccagcg ttatctaatt
     8161 gttttttagc ttctttagat aaataagcta aatctccaga gtcaagtctt atacccatag
     8221 gtttgtatcc tttttcagaa atatttttga atacttttat tgcattagga actccactgt
     8281 ttaatacatc ataagtatct acaagtagta ggcatttatc tggataaaca tcagcatatg
     8341 cttggaatgc ttcaagttca ttatcaaatt tttgtaccca actgtgagct tgtgtaccaa
     8401 ttataggtat atcaaacatt ttccctgcta gtacgtttgc agttccagca caaccaccaa
     8461 ctacagcagc cctagctcca tatatccctg catctggacc ttgtgcacga cgtaatccaa
     8521 attcaaatac agggtctcct tgagctgcaa agcaaactct agaggcttta gtagcaataa
     8581 gagactgaaa attaactatt gttaacaatg ctgtttcaat aagttgagct tgatataaag
     8641 gtgccttaac agtaattaat ggctcattag ggaacatgat agttccttcc tcaacagcat
     8701 atatatctcc tgtgaattta aattctttta aaaattttaa aaacttgtca gaaaataaat
     8761 ttaagttttt taagtactct aaatcttcat ctgaaaaatg aagattatct atgtattcaa
     8821 caacctgatc aataccacaa actatagtat accctccatc acaagcgttt tttctaaaaa
     8881 acatatcaaa tacaacaatg tcttcatgaa tattcttttc aaaatagcca tttaacatag
     8941 taagctgata taaatcagta agaagtgtta gatttctcat tatttctctc cttaattatt
     9001 attaaatatt aaaaattgta aaaactccca cacttatact aaaacataat atagtacaac
     9061 tttcaagtat aaagtattac ctaactatca taaaattatt taaagaggag gatgattata
     9121 ataggaaaaa tatggtttta tatggtatcg ataggaataa taggaagctt gatttctaaa
     9181 aatatgggtg aattaaataa agttatatta agtgaaacat caaaaggtat agagtttgca
     9241 ataagtctag ctggtatcat ggcactctgg atggggataa tgaatatcgc aaaagaatca
     9301 ggtttaattg aaaagatagg tcaaaaacta aatcctataa tgagaagatt atttccttct
     9361 attccaaagg gtcataaagc aatgtcttat atagttatga atattgcttt aaatatgttg
     9421 ggtgctggaa atggtgcaac agcatttggt ttaaaagcaa tgagtgagct tcaaactttg
     9481 aataacaaga aagatacagc cactaatgat atgattatgt ttatggtaat caatatatca
     9541 tctatacaaa taattccatt tactataatc aaattaagga tggatatggg ggctcaaaat
     9601 ccaagtgaaa taatttttac gactttattt gcaaccataa tatctacagc agtagcaata
     9661 ataacatgta aattttttca aagaagatat agataaaggg gatgtagata catggagata
     9721 ttttcaaact tacttatacc aataataatt ctgtacatag tagtttatgg taaatataaa
     9781 aaaatagatg tgtatgatag ttttgtaaaa ggagcaattg atggattaaa agcagcatgg
     9841 gatatcctac catatataat aggtatattt ttggcaatag gaatattcaa aactggtaaa
     9901 ggtcttgata tgttagaatg gctatttaca ccaatagcaa atatgatgag tataccaaaa
     9961 gaattaatag gtctaataag tgtaaaacct ttatctggaa gtggtgcttt agggatgtac
    10021 agtgaacttg caaatagagt tggaatagga agtttagtag aaaaaatggg tgctacgatt
    10081 gtaggttctt ctgagactat tttttacaca atggcaatat actatggaag tctgaagata
    10141 aaaaatacta gacatacatt atcatgtgct atgattagcc atgttgcagg tgtcatagca
    10201 gctgttttta tttgctatgt aatatttgta taatttgttg acaatatggg aaaaatttta
    10261 taatatagtg attatataga aacaaatagt aatacaaatt acaagctatg aaaagaagag
    10321 taaataaagg ttttttctat cagagagcta ggttaggtga aagctagtgt caaaagcttt
    10381 attgaagatg gtcttggagc tttttatctg agcaagtgtg ctagggtaaa acggttggac
    10441 acgttatagt ctactaaggt atctataaaa tgtttctaca tttcatgtga aacataacta
    10501 ttatagatga attagggtgg taccgtgaat aatctcgccc ctatgttaac tcataggagg
    10561 cgatttttta ttatttaaat gaaagaatca tatgattacg aacgataaaa aattaggagg
    10621 taaataacat gagcaaaccg agtttttatg taacaacacc aatatactat ccaagtggag
    10681 gattacacat aggacatact tattcaacag tagctgcaga tacaatagct agattcaagc
    10741 gtttctgtgg atatgatgta aagttcttaa caggaacaga tgagcatggt gaaaaaattc
    10801 aaaagaaagc catagaacaa ggaatgtcag agatagagta tcttgatgga atgataaaag
    10861 atataaaggc cttatggaat actatggata tatcttatga tgattttata agaactacag
    10921 aaaaaagaca tacagacata atacaaaaga tatttactaa actatatgag caaggtgata
    10981 tatataaagg tgaatatgaa ggtagatact gtactccttg tgagagtttc tggacagaat
    11041 ctcaattact agaaggaaat aaatgccctg actgtggaag agaaacatat ttagtaaaag
    11101 aagaatctta tttctttaga ttatctaaat atgaagacag attaaaagag ttatttaaag
    11161 atgatagttt ctgttttcct gctgctagaa aaaatgaaat ggtggcaaac tttttagaca
    11221 aaggattaga agatttaagc gtaactagaa cgacttttga ctggggtata aaagttcctt
    11281 ttgatgaaaa acatgtaatt tatgtgtggg tagatgcttt atgtaactat ataacagcat
    11341 taggttatat gactgataat gatgaagaat ttaaaaaata ttggcctgcc aatgttcaaa
    11401 tagtaggtaa agaaatagtg agattccata caataatatg gccagcgctt ttaatggctt
    11461 taggtctaga agttccaaaa caagtatttg gacatggatg gatactattt gctgatgata
    11521 aaatgagtaa atcaaaaggt aatgtggttt atccagaacc tataatagaa agatatggaa
    11581 tagacacact taagtacttc ttattaagag agtttgcatt tgggcaagat ggaagttata
    11641 ctcatagaaa ttttgtaacg agaataaatt atgatttagc aaatgattta ggaaacttaa
    11701 taagtagaac tgtagctatg gttgaaaaat acaataatgg tataatacca acagcaaaag
    11761 tttcaactga ttttgatgct gacttaaaag aacaagctgt ttcaactaga gaaaactttg
    11821 aagctgaaat ggataaaatg caattccatg aagcattaga gagtgtatgg aaattagtta
    11881 gaagaactaa caaatatata gatgaaacta tgccttgggc actagctaaa gatgaaacga
    11941 agaaagatga attagacaca gttttatata atttatgtga atctataaga ataatcgcaa
    12001 cactaataaa tcctataatg aatgagactg ctaataaaat atatgaacat ataggaataa
    12061 aaggtcaaga tgatataact acatgggaaa gtacaaaaac atttggtctt attggagaaa
    12121 atgtaaaagt atttaaaggt gaacctttat tcccaagatt agatgtagaa aaagagatag
    12181 aagaattaac taaaatgttc tctgggaaac ctccagtaga agaaaaacct ttagagcata
    12241 aagaagaaat tactattgat gatttagaca aaatagagct tagagtaggg aaaataataa
    12301 gctgtgaaaa acatccaaaa gcaaataaat tattagtatc acaagttaag attggaccag
    12361 aaacaagaca aatagtatca ggaatagctg aatactataa gccagaggat ttagtaggaa
    12421 aagaggttac agtggtatgt aacttaaaac cagttaaatt aagaggtgta gaatctcaag
    12481 gtatgatatt agcagcaggt gatgatggag acccatatgt tttaccattt acacaagggg
    12541 ctaaagatgg atgcgaggtt cgttagtagg taattaaata gtaaggagat taaagatgtt
    12601 atttgactca catgcacatt taaatgatga aagttttgat gaagatagag atgaacttat
    12661 tggttcatta aaagataaag gtgtagattt ggttgtaaat ccaggagcag atatagagac
    12721 gtcgataaca gcaattgaac ttgcgaaaaa atacgacttt atatattcag cagttggggt
    12781 acatccacat gatgtatcaa aactagatga tacagcaata gaaactctaa gaaaacttgc
    12841 aactgagaat gaaaaagttg tagctattgg agagattgga ttagattatt attatgatta
    12901 ttctccaaga gaagaacaaa aagagtggtt taagaagcaa attgaattag ctaatgagct
    12961 aaaacttcca ataataatac atgatagaga tgcacatgga gatacttttg agataataaa
    13021 gaatactaaa aatcctgaaa taggatgtgt acttcattgt tatagtggaa atgtagagtt
    13081 agctagagaa tatgttaaga tgggttgtta tatatcaatt ccaggcacgg ttacttttaa
    13141 gaataataaa aaaactagag aagtagtaag agaaatacct ctagaaagat tattcataga
    13201 gactgattca ccatatatgt caccagagcc acatagagga aagagaaaca atccttctca
    13261 agtatctttt gttgctgata agatagccca ggaaaaaggg atatgttatg aagaagtatg
    13321 tagagttaca aaagaaaatg caaagaaatt ttttaatata aaatagacaa attaaatctt
    13381 ataatgatat atataataag tcattataag tataggatga gagtgcctca aaatgagttt
    13441 tttagttcat gaggcactct ttttttatga aatcaaatat attttcatca tttcaacaat
    13501 gaaaaagaag tttatcatat tttgagacag tttgttttta tttgtttaat tcatatttta
    13561 aaatatattt tcttaacatt gttaaaaaag aatttacaag atattcatta gaattaactc
    13621 aactctaata ttcacttaac ttaagtggtt tattatatta ttaaagagaa gtaaacgaaa
    13681 ggtagattaa ggaggctaaa ttttgtatac agttgatagt actaagatat tgataaatat
    13741 gaacttagag aaactagaaa agatatactc aatgataaaa gatacatcaa gtatttctat
    13801 aattaaagaa cctaacttgg ctacagtaat ggtaagagct aatgaaagtg taaaaaatac
    13861 aacatttaat ttaggagaga tattagttac tgaatgtagt gtaaaggtag atgaaagctt
    13921 gggttacgga atagtttcag aaaacaataa taagaaagcc atttacttag cagttataga
    13981 tgcagtttta catagcaata acagtaaatt tgatgagtta aagaattaca taaataaaag
    14041 tgtatatgaa gagaacttaa ggtatgaaca agagattatt gatgaattta gcttgattaa
    14101 taaaacgaaa gttcaattca atgctatgga ttaaatagga ggagaatcat ggtaaattat
    14161 acacaagaaa tatatagaaa gatgctagat agtgcttcgt ttcctgggaa attaaataat
    14221 atagataata taaatataga aaataataca aaactattga atggagcaat tggtatagca
    14281 atcactttat tagaccaaga agttaccttc tatatagaaa attatactca aaaagatata
    14341 aaaaatataa gagcgcttac agtgtcaaat caaatagatt atagaaaatc agattatata
    14401 tttttagata ttaattcaga agtggatata ttacagatta aaataggttc ttttgaatat
    14461 cctgatgaaa gtgcaactat agtacatcaa gtaaatgatt tatcaacgta ctctcaaact
    14521 aagtatataa agcttgattt gagtggtcct ggtataaaat ctaaaaatag tatatatata
    14581 aatggaatta gagaagaatt tatagaaaga ttatctacca taaataaaga ttatccaata
    14641 ggaatagatt tgattttagt agataaaaaa ggagaaattg catttatacc aaggtcatca
    14701 aaattaagtt gggaggtatt gtagaaatgg cttatgtagc agttaaaggt ggagaagaag
    14761 ccatattaca ggctaaaaat atacttgagt tttatagggt aaaaggagat agcttgccta
    14821 tagatgtaaa gcagattgag gaacaaatgt cttatgctgt agacaaagta atgtctgaag
    14881 gaagtttata caacaaggaa ctatcagcat tggctataaa acaagctcaa ggtgatatat
    14941 tagaagcctc tttctatgta agagctttaa ggtcaacaat gcctagaatt acatattcag
    15001 aacccataaa tacagaagaa atgagaataa aaagaagaat atcagctact tttaaagata
    15061 taccaggagg tcagtatctt ggggcaacta gagactatga aaatagaata ttaaatgtag
    15121 aattattaga agaagatgaa aaaaagaata gagattttag agatagtttt ataaatgaat
    15181 tagaaacagt agatgaaatc cctgaattta caaaaatatc taatatattt agaaaagaaa
    15241 atttattaca agaagaagtt tgtgaaaaca aattggaaga agttattgat gatattacaa
    15301 gaaatagttt aagatttcca gtttctaggt cagcaaagtt acaggcattg actagaggtg
    15361 aaagtggagt agtgtcaggt tttgcttatg ctattgtaag aggttttggt gatgaacatc
    15421 catatataag tgaacttaga actggtaatg tatttataaa agtaaaacat cccatctatg
    15481 aagatgaaga aataataata ggtgatatgc tggttactga atgtgaaata gtttcaaaag
    15541 catcacaaaa taagactgaa aattctaaaa ctaattcgga agataaaaat aagatgtctt
    15601 taggatatgg tctttgtatg gggaataatg agaataaggc tatatctatg gccatactag
    15661 acaaatcttt ggagccaaat agtgaaaaac tagcacaaga tgaagaattt gtattgcttc
    15721 atatagatgg aatagattca ttaggattta tatctcactt taagcttcct cactatgtag
    15781 attttaaagc ggatgtagaa agaataaaat agtttgtgga ggtaagttta ggtgatatat
    15841 aactatggtt ttttagacga aaacacaaaa aaagaaataa gaagaaagat attaaaagca
    15901 atatcaatac ctggttatca agttcccttt ggttcaagag agttgcctat agctaaggga
    15961 tggggtactg gaggtttaca attaacacta tcattaattg gtaaaaatga tgtagtaaaa
    16021 gtaattgacc aaggaagtga tgattctaca aatgcttgta atataaggag ctttattgca
    16081 agtgtaagta atgtagaaac cacaaaagat acattagaag ctacattgat tcaaacgaga
    16141 catagaatac cagaagaaaa acttaaatct aatcaaatac taatatttca agtaccaata
    16201 ccagaaaccc ttagaatagt agaacctagt gaagtagaaa caagaagaat gcattcagaa
    16261 gaagattata gtaggatgtg ggtatatctt tatgaagata tagttagatt taatgatata
    16321 tctatagcag ttgaatatcc atgtaaagta aatgatagat atttaatgaa tccatcacct
    16381 atacctagat ttgatataaa aaaattaaat atgtcagaca atttattctt gtttggtgca
    16441 ggtagagaaa aaagaattta tgctattcct ccatatacaa aggtagaacc tttagagttt
    16501 gaggattata agtttgaaga ggaaaagttt gaaggaaaat actgtagttt atgtaacagt
    16561 acaaatacat ttttagatga agtatatgac agtgacacta atgaaaagta ctatagttgc
    16621 tcagatacaa gttattgtga aaaagttaga ttaaaaaata acagtataga tgtgacaata
    16681 ggaggtactt ggaatgagta aaataaatgt ctctaaaaga ttagaaaata tagacaagca
    16741 agtattaata ggaagaaata tcaatcttat ttatggagat gggtgtgaaa agtgttttga
    16801 aagcacagga tatgaaagca ataatatatg taagtattgt ggttctatag ttgcatgtaa
    16861 taatgtaaat ttagaacttt atgaaggaga agtccttgga attgtaggtg aatctggttc
    16921 aggaaaaagc actttattaa aaatactgtt tttccaagaa catgcaaata gtggagaagt
    16981 atatatatcc cattacaata aagacacaaa tatactagag ctttcagacc aaaaaaagag
    17041 atacataaag aatcatttta tgggtattgt gtatcaaaat ccccaccttg gtttaaattt
    17101 agactttagt agtggtggta atatagcaga aaaattattg atggcaaatt tatataatgt
    17161 tgaaaaaata agagatagag gcaaagaact tttaataaaa actaatatac ctgtagaaag
    17221 aatagatcat aaaccaaaat actttagtgg gggaatgcaa caacgagttc aaatagcaaa
    17281 agctctttca aataaccctc caatattgct attagatgag gttacaacag gtttagatgt
    17341 ttctgtgcaa gcggaagtat tggatttaat aagagaaatc caaagagaac ttaagatttc
    17401 tatgatagtt gtatcccatg actttgatgt tataaagatg cttgctgata ggacaattgt
    17461 aatgaaaaat ggaagtgtga ttgagtctgg tcttacagac caaataatgg aggacccaca
    17521 acacccatat actcagcaac ttataaattc attgctataa atatcgtttg taaataagca
    17581 aaatgggggt ttttaataaa atgaaagaaa ctatacttga agtaaataat tttagtaaag
    17641 actttgagtt acatgcattg aacaagacta taacggcatg tagtaatata agttttgaag
    17701 tttcaaaagg tgagttttta ggaataatag gtaaatctgg agcaggtaag tctaccatat
    17761 taaaaagtat ctataaaaca tatataccaa caactggaga aataattttt aattcagaaa
    17821 tatatggtaa tgtagacctg gctaaaattg gagaccgaga aatgataaat cttagaaaga
    17881 aagaaatagg atatgtatca cagtttttaa agacacttcc acgaataact gcaattgaac
    17941 tggtagtaca ttctttaatt gagagtgggt ttgaaaaaga ttgttcgtat gatatggcaa
    18001 aagatatact taaccaattt gaaataaagg aaaatttatg ggatgcgtat ccaaataatt
    18061 tttctggtgg agaaaaatta agattaaatt tagctcaagc tatggttaaa aagcctagac
    18121 ttttgttact tgatgaacca actgcatcac ttgataatca atctaaaata tatgtaaaag
    18181 aaaaaatgtt ggaacttaaa agtcaaggaa ctacaatgat aggaattttt catgatatag
    18241 agtttatgga aactgttata gataagacct ttactatggc aaaagggact ataagtgaaa
    18301 gtggggtagc ctagatgata aaactaattc ataatgcaaa tattgtatta gaagatatga
    18361 ttttagaaaa tggtcattta atcatagagg gagatacaat aaaaaaagta tcaaatgata
    18421 ggattgatta cgcatttgac tttaatgaga tagatgaaat aatagatgca aaaaacttat
    18481 atgtaatacc tggtattata gatattcaca gtgatgccat tgaaaaggaa atagagccaa
    18541 gaccaagtac attattacca tttaatatgg cattttatga actagataaa aaactacctg
    18601 caaatggaat aactacagtt tatcattcta tatctttagg ggatggagta ggagttagaa
    18661 gtattgataa ctctttaaag atgattaaaa atattgattc atataaaaat atagatagta
    18721 agagtataaa tcataaagtt catttaagat atgaagtgtt gtattatgaa ggtttagaaa
    18781 aggttttaga actattagat gaaaataaaa tagactactt atcaataatg gaccattctc
    18841 caggacaagg tcagtataca aatccaacat tttataaaga gtatgctact aaagtgtggg
    18901 gagttacaga aaattatgta gatacatggt tagatgattt agtaaatctg catgataatt
    18961 tagattggaa taaaatagct aatattatag gtatagccaa aactaaaaat ataaatgttg
    19021 catcacatga tgatgataca ttagaaaaaa tggatttcat aaaaaatata ggcataaatg
    19081 tatctgaatt tcctataact cttgaagttg caaaacattc aaaaaaattg ggtgttctaa
    19141 cttgtttagg tgcaccgaat attgtaagag gtaaatctca taataacaat ttaaaagcaa
    19201 tggatgctat attggaagat tgttgtgaca ttgtgtgttc ggattatctt ccatcagcta
    19261 tgataaagtc tatgtgtata gttgcagaaa gaataaataa tttaaataaa gcagtcagtt
    19321 tatttacctc aaatcctgct aaagcagttg gtatatatga tgaaagaggt agtataaaag
    19381 aaaataaaaa agcagattta gttttagtag atattgatag tgaatatcca aaggttttaa
    19441 atactattgt aaatggaaaa acagtttata aaagggaggt ataaaaattg ctaacagagt
    19501 tacattgtca tacaagtata tcagataata attttactac tgaagaaata ataaaaaaag
    19561 cagcagaaaa gaatataaat atcttagcaa taacaaatca tgacacatta tctggattgg
    19621 atgaagctct tgtgcttgga gagcattatg gaataacaat aataccaggt atagaaattt
    19681 cagcctatga ttataaaaat agaaaaagag ttcatattct tggatataac atagatttaa
    19741 acagtcaaga aataaaaaat ttgtgcaatc caatggtatg tgacagacat aaagcatctg
    19801 ttaagatggt aaataaaatt atagatttag gatacaaaat atctttggaa gatgtaaaga
    19861 aatattcatc aaagactgga atatttaaac aacatataat gaaagctctt atagataaag
    19921 gatatacaga taatatatat tcaagtctat ataaagaatt gtttcataga ggaaacggca
    19981 aggtatatgt atctttaaaa tatgtggatt acagagatgc aataaaagct ataaaaaatt
    20041 ctggtggaat ttgtgtgtta gctcacccgg gtcaaatgga taattttagc gctattgaag
    20101 agatggtaaa agtaggatta gatggaatag aagtatatca tccatctcac aacaaagaac
    20161 tagaaaaaga gtcgctttct tatgctaaga agtataatct agtggtcaca ggaggctcag
    20221 attaccacgg attttattct caacataatt atgaacttgg aagcaagagc ttaaataaag
    20281 aagatttatt taagtttagg ttagctttag gaggggtatt ataaatgtct tttttacaag
    20341 taaaaaatgt aggtaaaagt tatggacaag ttaaggtgtt aaaagacata tctattgata
    20401 ttgaaaaagg agagtttatt tgtttactag gtccaagtgg ttgtggaaaa agtacactgc
    20461 ttagaataat tgcagggctt gaggataaac atggtggaaa aataataata aatgataagg
    20521 atatgactaa ttcaccacct gaaagtagaa attttggtat agtttttcaa tcatatgcat
    20581 tatttccaaa tatgaatgtt tacaaaaaca tagcctttgg actagaaaat aaaaacataa
    20641 gtaaatctaa tattgataaa aaagttaaag aagttctaga cgttgtagaa ttaagtggat
    20701 atgagaaaaa atatccatca caattatcag gtggacaaca acaaagggtt gcattagctc
    20761 gtgcgattgc tttagagcca gactttttac ttcttgatga acctttatct gcattagatg
    20821 caaaagtaag acttaaactt agggagcaga ttagaagttt acatagaaaa ctaggcatta
    20881 caactataat ggtaacgcat gaccaagaag aagctctttg tttagctgat aagatggttg
    20941 ttatgaatag aggagaaata atacaagttg gaacgccaaa ggaggtatat aaaaatccag
    21001 aaacaccttt tgtagctgat tttataggaa caataaattt tatagatgat ggtattaata
    21061 agatagctat tagaccagaa gatataaaag tagagtctaa tagagattct aaagataagg
    21121 atataaaagt gggagaaatt ttagatatcg aatttagagg atttaactat agaattacag
    21181 ttgagtatcg ttctaagcaa atgaaattag atgtagtttc caaagttgca gaacaaatga
    21241 aactgtgcat aggttctaaa ataaacttta agatacctaa agaaggaatt gtacaatata
    21301 aatcagaggg atgtgcttaa tagctagggg tgaataaatg ataacatcaa aaaataaaag
    21361 aagaataagt gatagcacaa tacaaaaata tttcttgatt ttttcggtaa ttgtactaat
    21421 agtatttata ttatttccat tagtatcatt attaaaaaat gcatttatgg atacaagtgg
    21481 aaattttata ggaatttcaa actttagtaa atatgttgaa aatccaagtc ttatagcatc
    21541 atttaaaaat tcaatttttg tatctacagt ctcatcttta atatcactat gtttagcatt
    21601 tatatattca tacgcaataa caagaacaaa tataaagttt aaagattttt ttaaaactat
    21661 gggaatgttt ccactttttg caccaactat gttatatggt atttcactta tatacctttt
    21721 tggaaataag ggaatcttta caaccatggg tctggaagta ccactatatg gtccattagg
    21781 aataattatt tctcagattc tatttacatt tccacaagct tttttaatat tatctgtagc
    21841 actttcgatg gctgattata gactttatga agcagcagat agcttgggtg ctagtgaatt
    21901 taagaaattt tgcaaaataa caattcctgg tataaagtac tcattactta gttcattctt
    21961 tgtatccttt atacttgcat ttacagattt tggagcagct aaggtagtcg gaggaaacta
    22021 caatgtactg gctactgaca tatataaaca agtagttgga caatttaaca taccaatggg
    22081 agctactgtt tctatggtaa tgttaattcc tgtactaata gcatttacat tagataagat
    22141 ggcaagtaag aaacaaggta tgaccataac tgcaaagtct atgccatata agattaagga
    22201 aaataagttt agagatagag tatttttaat agtatgtagt tttataacat tgctaatttt
    22261 tattttaata ttcgtttcag ttataggttc agttataaag ttatggccat ataatttaag
    22321 ctttacaatg gaacactata catttaaagg tattttggga agtggaataa aaacatactt
    22381 aagcagtttg aaaatatcaa ctttaacagc aatatttgga actatatttg tattttttag
    22441 tgcttatata atagaaaagg tggataagtt tcctaaatta agacaaacag cttacctttt
    22501 atcaatgata ccaatggcct tacctggctt agttttaggt atatcatata taactttttt
    22561 taactcagca agtaatccat taaatttttt atatggaagt acagcaatat tagttatagt
    22621 aaatgtagtt catttttatt cagtttgttt tataacctca aattcatctt taaaaatgtt
    22681 ggataaagag tatgaattag ttgcaaaatc tataaatatt ccattctata aagtattttt
    22741 taatataaca gttccgatgt ctataacatc aatacttgaa atagtagtat attattttgt
    22801 aaattcaatg gttactgttt cagcattaat atttttatat atacctcaaa ctcagactgc
    22861 atcaatatca atactaaagt tagatgagat aggatatata ggaccatcag ctgctatggc
    22921 agtattagtg ctattaacaa atataatagt aagacttttg tacgaatttg taacaaagaa
    22981 attaaaaaat aacacacaaa aatggcagca aaaagatgtt gcatagggga ggaaaaaatg
    23041 aaatttaaaa agctagtttc tttaggagta ttatcaacac ttttattaag tgtaataaca
    23101 ggatgtagta ctgattcatc aggttcagat tcaaagtctg atagtaagac aaaaggggat
    23161 ttaacagttt acacagcaat tgaagaagat agtatagagc catatttagc tacatttaaa
    23221 gagaaatatc cagatattaa attaaatatt gttagggcat caacaggaga tataacagca
    23281 agattattag cagaaaagga taatcctcaa gcagatgttg tatggggagt tgcagccaca
    23341 agcttattgg ttgcagatga ccaggggatg ttagaaccat atgctccaaa aggttctgaa
    23401 gaaatagagt ctgcatttaa agatgataaa gaagttcctt cttgggttgg gatagatgct
    23461 tggatgacag gtataacagt aaacactaaa gaattaagtg ataagaacat cccagttcct
    23521 agttcttacg aagatttaat aaagccagaa tataaaggtc ttatatctat gcctaatcca
    23581 tcttcttctg gtacagggta cttaacagta tcagccctta tacaaattat gggggaagaa
    23641 aaagcatggc agtatatgga taagttgcat gagaatattg gtgtatacac tcaatctggt
    23701 tcagctccag ctacatcagc agcttcagga gaatatccta ttggtatatc ttttggatat
    23761 agaggtataa aattaaaaga ggaaggctat ccagtagaag tagtatttcc aaaagaaggt
    23821 tctggatggg atatagaagc aaatgctctt gtgaaaaaag ataatataaa agaagcatca
    23881 aaagtatttt tagattgggc tataagtaaa gatgcaatga atgaatactc taagaactat
    23941 gcagttacta ctatttctac aggaaatcca ataccagaag gattccctaa aaagccatta
    24001 gagcaaatga tagataatga tttaaaatca gcagcaaaaa atagagaaga tatactaaat
    24061 aaatggatat caaaatatga tggtaagaca gaaaaagaga gttaagtaaa actatacaaa
    24121 tggagtaaaa agaaaaaatt tttttaagta gattttatta taataatata agttgagaat
    24181 aatataggtc aagaaaagtg aatattctat aaaaatgtat agagtaagta taccattcag
    24241 tttgaatggt atatttttta tttttaga
//
LOCUS       DAGQWR010000060        24099 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.060, whole genome shotgun sequence.
ACCESSION   DAGQWR010000060 DAGQWR010000000
VERSION     DAGQWR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24099
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.060"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(150..2045)
                     /locus_tag="MIW95_002002"
     CDS             complement(150..2045)
                     /locus_tag="MIW95_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBY3151329.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKSETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            complement(2671..2814)
                     /gene="scfA"
                     /locus_tag="MIW95_002003"
     CDS             complement(2671..2814)
                     /gene="scfA"
                     /locus_tag="MIW95_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBY3151330.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(2880..3242)
                     /locus_tag="MIW95_002004"
     CDS             complement(2880..3242)
                     /locus_tag="MIW95_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBY3151331.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(3300..3593)
                     /gene="yajC"
                     /locus_tag="MIW95_002005"
     CDS             complement(3300..3593)
                     /gene="yajC"
                     /locus_tag="MIW95_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBY3151332.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(3727..4848)
                     /gene="tgt"
                     /locus_tag="MIW95_002006"
     CDS             complement(3727..4848)
                     /gene="tgt"
                     /locus_tag="MIW95_002006"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBY3151333.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITSIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPQDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(4927..5412)
                     /locus_tag="MIW95_002007"
     CDS             complement(4927..5412)
                     /locus_tag="MIW95_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151334.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(5424..6449)
                     /gene="queA"
                     /locus_tag="MIW95_002008"
     CDS             complement(5424..6449)
                     /gene="queA"
                     /locus_tag="MIW95_002008"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBY3151335.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFLYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGNNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(6767..7786)
                     /gene="ruvB"
                     /locus_tag="MIW95_002009"
     CDS             complement(6767..7786)
                     /gene="ruvB"
                     /locus_tag="MIW95_002009"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBY3151336.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(7893..8495)
                     /gene="ruvA"
                     /locus_tag="MIW95_002010"
     CDS             complement(7893..8495)
                     /gene="ruvA"
                     /locus_tag="MIW95_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBY3151337.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASSAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(8488..8997)
                     /gene="ruvC"
                     /locus_tag="MIW95_002011"
     CDS             complement(8488..8997)
                     /gene="ruvC"
                     /locus_tag="MIW95_002011"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBY3151338.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(9309..9938)
                     /locus_tag="MIW95_002012"
     CDS             complement(9309..9938)
                     /locus_tag="MIW95_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151339.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKNNGVQTSK
                     VVSASANMDEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMNATIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            10141..10464
                     /locus_tag="MIW95_002013"
     CDS             10141..10464
                     /locus_tag="MIW95_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBY3151340.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            10732..11586
                     /locus_tag="MIW95_002014"
     CDS             10732..11586
                     /locus_tag="MIW95_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151341.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(11772..13208)
                     /locus_tag="MIW95_002015"
     CDS             complement(11772..13208)
                     /locus_tag="MIW95_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBY3151342.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNELLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTHGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKEMLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(13279..14661)
                     /locus_tag="MIW95_002016"
     CDS             complement(13279..14661)
                     /locus_tag="MIW95_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBY3151343.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAVLIGGWIYGKRFEKYPAYAIDENDREKN
                     LEKGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKRYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(14797..15702)
                     /gene="garR"
                     /locus_tag="MIW95_002017"
     CDS             complement(14797..15702)
                     /gene="garR"
                     /locus_tag="MIW95_002017"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBY3151344.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            16098..16763
                     /locus_tag="MIW95_002018"
     CDS             16098..16763
                     /locus_tag="MIW95_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY3151345.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDVYKTTLIHMETPKYINLEDFC
                     NNK"
     gene            16871..17419
                     /locus_tag="MIW95_002019"
     CDS             16871..17419
                     /locus_tag="MIW95_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151346.1"
                     /translation="MIFLNENVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTIEILRKVLFERSEERAIAMRERALENGDELIADNIISTTDVPFLGWVPEFKE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            17681..17989
                     /locus_tag="MIW95_002020"
     CDS             17681..17989
                     /locus_tag="MIW95_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151347.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(18151..19941)
                     /locus_tag="MIW95_002021"
     CDS             complement(18151..19941)
                     /locus_tag="MIW95_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151348.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(19941..21668)
                     /locus_tag="MIW95_002022"
     CDS             complement(19941..21668)
                     /locus_tag="MIW95_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151349.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSARVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(21913..22477)
                     /locus_tag="MIW95_002023"
                     /pseudo
     CDS             complement(21913..22477)
                     /locus_tag="MIW95_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
     gene            22766..23878
                     /locus_tag="MIW95_002024"
     CDS             22766..23878
                     /locus_tag="MIW95_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBY3151350.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     AEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
BASE COUNT         7914 a         4129 c         2822 g         9234 t
ORIGIN      
        1 caatctaatt ctaaatgaga caccttattt ttattatttt atcttgttat ttgtatattt
       61 tataatatct acactatgtc ttacatatta acgcattgta taatcatata ttatctaaat
      121 aaacttttaa tattaattcc tatatcttat tagttcttag taaataagtt tattttttct
      181 gctatttttt caatagcgtt tttagctatt ccttcaccta tttgtactaa cttagtagca
      241 ttcttaagtc ttaactcaac tacttcttct tgtgatttag aaagttcttc tgtagttaat
      301 actattggag cctttgaact agctgcaagt ggtgctgctg taagtgcatc aactaattgt
      361 ccatttccac ctacataacc atcttttgct acatatgctt tagttgcacc tgcatagtat
      421 ttattgatta ctttagcatt agtatctttt ctattttctc cagctaatct ttcaacttta
      481 ttatttaaat ctatatcaac taatttgtct tcaacttctt ttgatacaga attttctcca
      541 cccactattt ctaattcttt tccttttaat aatttttcag catttgcatc taattcgtta
      601 cctgtaacta ttattggagc accaaattga gcagctctag ctgatatact catagcatca
      661 gcttctccat ttccacctac tacaaaagca gtcttaaatg tatcttttaa ttgttttgct
      721 atttctaaag aagtttcaaa tctatcttct ccagaaactc tctcaacttt acttacacct
      781 aatttagaaa gtttttcttc agtttctttt gatactttag attctccacc tactatatat
      841 attttcttag aagatagatt acttccaagt actcttaata tttcagcttc tatttctgat
      901 ggtagtgaat cttttttaga taataatatt ggtgcatttt tttgagaggc aagtggtgct
      961 gatgctaatc catcaactat agcatcttct ccaactaaaa ttatagcttc tgcttctcca
     1021 tctttgaatc tctctttact aacttctact gctgttgtaa atcttgaatc accagataaa
     1081 acctctactt ttaattcttt tccatctttt acatcagtta atactttgtg taagtctcta
     1141 actaacttat ggtttttact tgaaacactt attgtttctg acttttcatt tgccttgctt
     1201 aaaactatat caaagcttga tttttctggt ttatctttat catcactatc ttcttgttct
     1261 tgagtaagga ttgcttcatt ttcaaatgtt aaagcatatc catttttaac tttattaact
     1321 atttctcttc ctaatgtagt taatctaatt ccatcaaata attcttctgc tgatatgtct
     1381 tgtaaatctg cgtttttaac tgttatcgta tgtaattctt ttgaatctat gtcactataa
     1441 ctcttttcat aaccttcaaa agtaccttct tctgatgtta tcacttttga gaaatctaaa
     1501 tgttggtctc ctacatttaa tgtaataaca tttgctgaat cttttgctct ttttacttca
     1561 actgtatttg tagattttat agttgctgtt aacttgttat cagaatcttc tgctaaagtt
     1621 gcattgtaag cattaactgc atctactatt tcttgagcag ttttgtagtt ttcaatttta
     1681 tagtcaacta cttttccacc taacacttga tgtcctttat cttgaatagt aacttttaaa
     1741 ctttgccctt ctgttaaaga gtttatttta ttttgtaatt gagttgcatt agttaagttt
     1801 acattgtcta cttttatatc gtaaactcta tcatttacat ttgctcctgc ttttgtatct
     1861 tcaaacttaa cttctaatgc ttttctaacc tcttcgatta gtttagcaga atctttcgca
     1921 cttactgtgt aatttttatc aacattttct gtcacagtat tgtctgcgaa tattggtgct
     1981 gctgcactca taactgtagc acctgccatt actattgcca tacttctctt tttgttgctt
     2041 aacatattta tgtcctccga atattatttt ttatttttta tattcgtatt tcccacacat
     2101 aaataatatt atatataata ttatttttca atatatttta tgtgtttaca taatttacta
     2161 acacaaacac taataatatc ttcttgaacc ccttccccct acttagctct ctataattat
     2221 gctcaaagat agaaatccct attttatagt gattttctac taaacaatca tatactaaca
     2281 aaagtattca aactccattt ttatatacta ctatgcacta atttttgctt tgctcttatt
     2341 acttaaatat atatcactaa attaatatct acctccatat attaaaatat ataacatatt
     2401 ataaaatcat cataaattag agtttaaaat taaaaattat tctaatacat gtaattgata
     2461 aaaaataaaa tataagagta gctactttaa aataaatgct cttatatgat tagatatttt
     2521 tgcataatta aacgataaga aatcaaattt aattttacat aagtagaata tatattttta
     2581 tataataatt gaaaataaaa gaggatacct cataatgata aaagaatcac tttgagatac
     2641 cctcttacaa acaactaaat caataatttt ctatctttca cactcttgat ttgcaactgt
     2701 acaagaagtt ttacaagctg attgacaaga agtttgacac tctccacatc caccttttgc
     2761 agcactttgc tttaaagtag cttgagataa agtttttaca tgtttatttt ccattttcaa
     2821 cgcctccatt tctaattgtt tcctattgat tatatcataa aactacttta atataaactc
     2881 tatttaaagt tgacacccaa tacgcctcca atgcctcctg ctgctgatat tagcaataac
     2941 ttataaatca tccctacttc aaatacaaac ttttcttgtg ctaaaaacac agtcaaaaaa
     3001 atacatacta tgtacaaaag tcctactaaa agtccataca taagtccttt ttctttcatg
     3061 tgttttgaag tgtaaaaccc tcctatagct gcagaaatag tagttataaa tgatgagacc
     3121 atagttatat tatctccacc aatatctgta aatgtcaaaa ataagttata cactaataaa
     3181 acagccaaag ttattatata tgcatatcct agacctttaa aaatataatt agattttccc
     3241 ataaaaaaac ctcctctcaa attaatatat attcgagaaa aggcttttaa attactaatt
     3301 tagtcttctt ctatttcttc ttttttagat ttaacagttc ctatagccca tttttcaaat
     3361 ggaactttag ttctatttgg tcctaattct aatattacaa catcatcttc aactttagct
     3421 acattagcta ctattccacc tattgtaatg accttgtctc ctacacttag gctacttctc
     3481 atttctttta attgtttgtc ttttttcttt tgaggtctaa tcattaggaa ataaaagatt
     3541 gcgattacta ctacccatag acctatactc ataattattt gttgctgtgg cattaatttt
     3601 tcactccttt gtaaattata ctcttttgta aatcataata aatgtttata taacaattta
     3661 ctttctctat ctttgagtac tattgaatat tatatattaa aatgattata attaatagtg
     3721 caaaacctat atttcatatc catattttgc aaaaaattca tttctaaaat caagtaatct
     3781 atcttccatt atcgcttgtc ttatttgttt cattaaattt aataaaaagt gaagattatg
     3841 agtagttatt aatcttgcac ccagtatttc atttgctttt ataaggtgtc ttatataagc
     3901 tcttgaatag tttttacaag cataacaatc acattcaggg tcaagtggag taaagtcttc
     3961 agcataagtt gcatttctaa ctactacttt accttggcta gtcattgcag ttccatttct
     4021 agcgatacga gtaggtagga cacaatcaaa catgtctact cctcttataa caccttccac
     4081 taagtcatct ggactaccaa ctcccattag gtatcttggt ttatcctgtg gcataagtgg
     4141 agttgtatgc tctagcacgt catacataag tggttttggt tctccaacac taagcccacc
     4201 aatagcatat cctggtaaat caatactagt tatttctttt gctgattgtt ctcttaagtc
     4261 tttatacatc ccaccttgta ttataccaaa taaagcttgt ttgtcggtat tattatgggc
     4321 atctttacat ctttttaacc atcttgttgt tctttctaaa gaatttttta catattctct
     4381 atctgatggg tatggtgcac actcatcaaa cgccatcatt atgtctgaac ccaatgcatt
     4441 ttgtatttcc atagcttttt ctggagttag aaaatgtttt gaaccatcta agtgtgacct
     4501 aaattctaca ccttcttctt ttatctttct taaaggccct aagctaaata cttggaaccc
     4561 tccactatct gtaagtattg gtttatccca gttcataaac ttatgaagac caccagctct
     4621 ttttataagt tcatgacctg gtctcatata taaatggtat gtgttactaa gtattatttg
     4681 tgaaccaatc tcttttaatt cttctggtgt catagatttt acagttgctt gagttccaac
     4741 tggcatgaat attggtgttt caataatccc atgtggagtg tgtaatcttc ctaaccttgc
     4801 accactctgc ttacaagtct ttatcaattc atatcttact gcgtacataa agttctcctt
     4861 aaccatattt atttctcatt atttcttaag atatatttgt aataagctat tcatataatt
     4921 tattgtctaa atagatttat cttcgtcttc tttgttctgt tgctccttaa ttgcttcttc
     4981 atcatgaata ctcattatat taagagcact cttgtctttt gttactaaaa acttttttat
     5041 agcaaatagt cctgctatag ctataactcc tattaaaaca tctatcatac cttcattttt
     5101 aggcagtagc aacatttttc tagctattgc gtaaagtaat gcctcaataa atgctcctgg
     5161 tatatgaaga gatagcatta ctacaagttc aacacctatt actaaaagta atatttgtgc
     5221 taaaaaatca ttaagctgac tgtattgtac tggattttga aaatcaacta tatatgctcc
     5281 ccacataagt cttagaacat ctatagttcc taaaaatact gctattagaa ctactactgc
     5341 taaaacacgt tcaaatataa aagcaatctt taatataggg ttattctttt tttgtttttg
     5401 taaactatgc attcattacc tccctacttt ataatcatag catctccaaa actgaagaat
     5461 ctatatcttt cttttactgc ctcattatat gcatttaaaa cattatcctt tccacaaata
     5521 gcacttacta acattattag tgtagattct ggtaaatgaa aattagtaat aagtttatct
     5581 aaaactttaa atttataacc tggatatata aatatttctg tccatccact agtttctttc
     5641 atctttccat cctcattaca agctgattct atagttctac aactagtagt accaacacat
     5701 attacattgt ttccattttc cttcgctcta tttattttgt cagctgcttc ttggctaacc
     5761 atataatatt ctgaatgcat cttgtgattt aaaacatctt ctacttttac tggtctaaaa
     5821 gtgcctaaac caacgtgtag agttacaaaa gctatatcta cacctttttc ttttatttta
     5881 tttaacaatt cttctgtaaa atgtaatcct gctgtaggtg ctgctgctga accattatgc
     5941 tttgaataaa cagtctggta tctttctttt tcatctagtc tagcagtgat atatggaggt
     6001 agtggcatat ttccaagttc atccaatatt tcttcaaaaa ttccatcata aaggaatttt
     6061 ataattcttg aaccttcatc tgataaatct acaacttctc caattagctt tccatttcca
     6121 aaagaaaatt tagtccctac ttttgctctt tttcctggtt ttactaaagc ctgccaagta
     6181 tcttcctctg ttctttttaa taataaaaac tctattttac ctccagtttc aagcttctct
     6241 cctattaatc tagctggtat aactctagta ttatttaata ctaaacaatc tcctggattt
     6301 aaatattcta ttatatctct aaatacttta tgttcaatat tacctgtctc tttatctaat
     6361 accatcaatc ttgagctagc tctatcttct ataggaactt gagctataag ttcctgtggt
     6421 aagtcaaatt taaaatcact tgttttcaat gttaaacttc ctctcttaaa atttctccag
     6481 ctacaataat atcaaaaaaa caacttattt tccacaatat taccataatc tatcaagaaa
     6541 ttgctatttt taacagacac catatccaca atatatagat atttttccac gatatacatg
     6601 tcttatccac aatatataga tacaatacac acaattttta tcgtgtttat ccacagtatc
     6661 tacagatata attaccattt tgtggatata tttgaatttt tagccatttt agaaatattt
     6721 taatttaatg actcttttat tttataaata ctcgtataat ctatttttat ttttcattag
     6781 gatatggaat ttttaagtgt tcatatgcta atggcatggc tactcttcct ctaggacctc
     6841 tatttataaa tcctaattga agcaaatatg gctcatatac atcttctatt gtatttctat
     6901 cttctccaat agaagctgcc aatgtatcta aacccactgg tcctcctcta aatttttcta
     6961 ttatagtcat aagaagcttc tcatctacaa aatcgagacc caaactatcg actcctaaaa
     7021 gttccagagc atcttttgcc accttatcag ttatttttcc atcagctctg acttgtgcaa
     7081 aatctctaac tctttttagt aatctatttg ctatccttgg agtccctctt gaacgttttg
     7141 caagttctaa tgctccatta gactgtatct ctgcatctaa gatgcttgaa gacctaagta
     7201 ctatttttga aagttcatca actgtataat aatccaattt acaaataaca ccaaatctat
     7261 ctctaagtgg atttgtaagc ataccagctc ttgtagtagc tccaataagt gtaaatttag
     7321 gaaggtcaag tcttatgctt ctagcagatg gccctttccc tattattata tctaggcaaa
     7381 aatcctccat tgctggatat aaaacttctt caacacttct attgattcta tgaatctcat
     7441 ctataaataa aacatcattt tcatttaaat tcgtaagtat ggctgccaaa tctccagctc
     7501 tttctattgc gggtcctgac gtaattctta aatttacccc catctcattt gctattatac
     7561 ttgctagtgt agtttttcca agacctggtg gtccatataa aagcacatgg tctaactgct
     7621 catttctcga tttagctgct tctataaata tactaagttg ttctttggat ttttcttgac
     7681 ccaaataatc ttctaaagtt tttggtctta aactgttttc tatatctata tcttccatct
     7741 tcattgtaga agttattatc ctattttcat cttcaaaacc ttgcatttat acccctcctc
     7801 tcgtactttt gaaaatctac tttatatatt ttcaaattta cataaactcc ttaaatattg
     7861 ttttaaaatc caaatattat aaaatccaaa tactatttca aggatttact cattatatat
     7921 gtaagagctt tctttataat agcttctgta ttcataccat cttttttaca tttttgaact
     7981 gcctcttttg attccgacaa agtatagcct aaagctacaa gtgcatctac agactcatct
     8041 tgtgatatca aggtaggttt ttgtgataat aacgttggtt caaactctat gttatttttg
     8101 tctaccttat ccttaagctc taagacgatt ctttctgctg tctttttacc aacaccagga
     8161 gctttggata attttccaat gtcttcactt aaaatatatg ctcctaactg tgctgatgat
     8221 gcaaatgata gtattgaaag cgccacctta ggtcctattt tagatacaga tgttagtaat
     8281 tcaaacatct taagttcttc cctacttaca aaaccacaaa gactcatatc atcttctctt
     8341 acaattaatt tagtatatat ttttgtggct tctcccactt gtaaattcat tattgtattg
     8401 gatgatacat ttattttata tcctatccca ttattttcaa caactatact gtctatataa
     8461 atttcttcta ctgtaccttt tatataacta tacatactta ccacctatat tctttagagt
     8521 tttttcaagt ttgtttgaat gagcatggca aatagccaca gctaaagcat ctgcaacatc
     8581 atctggtttt ggaacttttt ttagacttaa aaatgaagtt accatctgtt gaacttgtgc
     8641 cttatctgct ctcccatatc caactacacc ttgttttact tgaagtggag tgtattcata
     8701 tacaggcttc ccattatgag cacatgcaag catagtaaca cctctagctt gtgcaactgt
     8761 tatagctgtt tttacattct tgttaaagaa taattcttcc attccaactt catctatatt
     8821 gtaattcttt actattgtat caattccttt atacacaagt tccaatcttc tcgatatatt
     8881 catatgggca ggtgttgtaa ctgctccata atcgattact ttaaacttgc tatttttgta
     8941 ttcaattata ccatatccaa ctatggctat acctgggtca atccccaata ttatcatttt
     9001 ttaaaattcc ccctaaaaat aaaagaaata ccaaaatgta tttctcataa aaacttatgt
     9061 tctaaatcat tatatcataa ataatattaa caaatcataa aatgctctat gtaaaatata
     9121 aaatcaaata aaaaagttat atcaaagcaa gattttacct caatataact tttatattta
     9181 ttacaaataa attattgtat ttttatagga attaaatgtg actacaattt taatactaat
     9241 ctatttaata ttaaattatt tattattaac ttacttagta tttaacttga aataatatta
     9301 atttactatt aacttgcaaa gtcttctaat cttgaatagg cctcttcttc agtatccata
     9361 actacacctt ttttgatttc atcttgaact gttttaggaa gtgaatctac tcttattttt
     9421 gttttttcta ctaatgtttt acttccacta tcatcatatt tatatagtgc tatatttcca
     9481 ttatcatttt caatagtata tttacctttt atagtagcgt tcattgcttg tacatcttta
     9541 ttatcactat atttagattc taccaatatg atttcatttt tatccatact ctttattttt
     9601 ttagttggat attctttttt aaaatatgat actatttctg attttgtctt atctaataat
     9661 gactctggaa ctatcccagc cattgtaagt gttctcttat agttagtttc attttcgcct
     9721 tcactttgaa gccaaacttc acaattttta tttaatccaa aagcttcttt tgacttttcg
     9781 tccatattcg cactagcact tacaacttta ctagtttgaa ctccattatt ttttaatctt
     9841 gtccctatat aaactccgct acttaataca aatactccaa ccagtattaa ggcagatatt
     9901 ttccaaggaa ataattcttt tttcttcttt tcaaacattg attacgcctc ctttttctat
     9961 tattttctcc aagctgtaag tttttataca atttaggtaa attttagaag aaaatatatt
    10021 tatatttcaa attactctaa gaatagatat tttctaaaag gtagttttct cttaatggtt
    10081 atattcttgt aattatatgg acataattta taataaataa tcgtttagga ggtaataact
    10141 ttggaaagaa taaactgtaa tgtaattaac tgttcacata acaacagcca tatatgttat
    10201 gcagataaaa tttttataaa tggtgaaaaa gctcacaatg acagacatac ttgttgtagt
    10261 tcatttttag atgaatctat ttatagtaat ttatctagca acgttaataa tagaaattgt
    10321 gaggaattag tttgtactgt aaaaaattgt aaatacaaca aagatggtag cttatgtaca
    10381 cttgataata tattggttgc tcctgaaact gatagagtaa acctttatat tgaaacatgt
    10441 tgttctagtt tcgaatgtaa atagtcatag tataaatata agggtgtcaa atgtaattaa
    10501 actaacatag tgtagagtat ctacaactat tagtttgatt atacttgaca cccttattta
    10561 ttttaaataa aatttcatta ttataaccat gtttataatg ttctaactgt aattaaattt
    10621 gataaatttc tataaattgg tataatcaat atgtaaattg aatttagcaa attaatcata
    10681 aaaataaaat atcgagtata tagtgttata ctaactagaa atgaggtgct tatgagtaca
    10741 ttttatattc aatttcttta ttttgtaatt tatagcttta taggatggtg ttgtgaaact
    10801 acatattgtt cagttttaca aaaacaattt gtaaatagag gttttttaaa tggtccattt
    10861 tgccctatat atggatttgg agcattagca atagttgcta ccttgactcc ttttgtacac
    10921 aatattcctt tgttattttt gtttagtata attataacta gcataatgga gtattgtacg
    10981 agctttattt tagaaaaaat atttaatatg acttggtggg attattctaa acataaattt
    11041 aatattcatg gaagagtttg tctggaaaac tcacttatgt ttggtgtatt atcattgatt
    11101 gtaatgctta tagtacatcc tattgtggtt gactttatta actctatctc taaaaatgtg
    11161 ctatttatat ttgctatatc tatagaaatt tattttgttt tagatttagt tatcacagtc
    11221 catacaattt tacaattaaa tggtaagtta aaacaaatca atcttataat taaggaatta
    11281 aaagataaga aagaatacta caaacttatt acacaagaaa ctatagaaaa caaacttgaa
    11341 tctttagttg aaaatagact ggatactttg gaagatagat ttgaaatact taaggaaaaa
    11401 gaaaactatc gctatgctaa aaatcgtatt gatgagctaa aaaataaact taatactttg
    11461 ctgtctaacc acaaattact tcatagacgt ataatcaaag cattccccaa cataagctct
    11521 aataagcata cagatatttt aaataaaatc aaagaaaata ttaaatcaat taaaaaagat
    11581 aaataataaa ataaacacat aagttaaata ataaaaatac taatacataa gattaaggtt
    11641 tagaatattt tttgtaaaat actctaaacc ctaattaata tactattatt tttaatctta
    11701 atttataatt ttagtttatt ttacttttaa cacagtaaat ttaagcgttt aaacttcaat
    11761 acactaatat actaccccat caatatctca actgattctc taagtgtgct aatctctccc
    11821 acatttccag ggaaaattat atatggcata ttaggaaatt tgctttcttt gcctgtcatc
    11881 catactggta taccaggttt tatctgtccc attactgtag ctttttttac tcttagtgct
    11941 ttagtaccaa catcactaga agtaattcca cccttagcta taataaaatt tggtcttaca
    12001 ttaagcatac caattatact ggtaactgca tctgatattt ctacagaaat cataagctgt
    12061 ttatctttat cattcgtatc caagtcaaaa cgctctcgtc tagtataaac agcaacagtt
    12121 ttaccactgc ttattttttc ctctacaact tttataactc tttttacctc attttttaga
    12181 ccattttcct gaagaactaa atgttgatta aattctataa attctattgg atatttacaa
    12241 tttttaagtt cttctaactg ttgggtagtc ttattcacat gtgaaccaac aagtataatt
    12301 cctccattgg tattaccttt tgatactaac tcatccttag taagcatttc cttatcactc
    12361 actccgccaa gtacttttgt tatggcagca gcgcttctaa acataaattc cttaccacta
    12421 tttactgctc gtataaacgc aattgtaaat acctttacat ctacataatc ggttgcattt
    12481 acaataattt tattgaatcc ttcagctttt ttaagttttt ctgttatagc ttcaatattt
    12541 agctctctaa gttctttaga agaaatatat atcatatcac tagatttgta ctttccatgt
    12601 gtcttttctt cacaccactc acctatatca gatgatttat atccaaaaga tttatccttt
    12661 gcaaattcag tcattcctgc tggtacaagt atttctcctt ctttaacata atgtacatta
    12721 tctatagtaa acctaccacc ttctttaaaa aatggcatta ttatttcacc ctgaaataac
    12781 ttccccgata atctctctac ttcgcttttt aataaattag tttcaacagg ataatgtcct
    12841 ctcatagtag aatcactacg acttatgatt ataaaatcct tattaacttt ctttgataca
    12901 tccacaatat tctttgaaat ttctttatgt gcttttgtag tttgagcaac agtaaatccc
    12961 cttgaattag taagtataaa aaacatacta tttttctcat taaaaccttg ttccatacta
    13021 tccttatccc aatcagtata aacagaaata tcatgaactg tttgtacacc tgttgggtca
    13081 tcatctaata caacaatttt tctatctaaa tgcttaagct catctgcaag aagttcattt
    13141 aatctgtcct catctacttg tttaaaagta ttaagtatat ctatgctaat tggttttact
    13201 tctctcattt aaaacgcccc tttatgtaat ttgtatagtc taaatgtaat ttatataata
    13261 taaacaatag tttgttattt atagacctgg aagtggtata attccattta taacactaag
    13321 caataaaacc attataaatg ctacaactcc agcaatggtt cctccaattg tccaagagtt
    13381 taaagtatct ttaactgata atccactaaa tcttgaaagt acccagaatc cagaatcatt
    13441 tggaagggag aaaccaactc ctccagcaca tatagcaagc cctactaaaa ctggtgacat
    13501 tccagttgcc aaaattgtag gtcctaaaat tgatgatgtt gttactaagg ctacagtagc
    13561 agaaccttga gaaattctaa gcaaagcact taataagaat ccaagcacta caactgatat
    13621 actaagacca gacatagtat caactaagaa gttacctata ccacttgcat ttataacact
    13681 gccaaacgca ccacctgaac ctgttatcaa taatattagc cctgctgaat cagcagcttc
    13741 aataactaca tctcccatag gtttatttat gtatctcttt aaaaatccta ttgccaccaa
    13801 tattccaaga agtattgcta tgtttttatc tcctataaat gcaagtactc cactcattgt
    13861 agaacctttt tctaagaaca tactaaatac atttgatata agtataagaa gtattgggaa
    13921 caataaagaa aacatagaaa ttccaaaacc tggtacttta gaagaatcat catcttcaat
    13981 ttttatgcta tcttttccct tttctaaatt tttttctcta tcattctcat caatagcata
    14041 agctggatat ttttcaaaac gttttccata aatccatcca cctataagaa ctgctggaaa
    14101 agcaacaatt atagaataaa gtataaaact acctacattt gcatttacat ttgctgcaac
    14161 tgctaaaggt cctggtgtag gtattacaag tgcatgacct actatagtcc caactcctaa
    14221 ggcacataca aaaaccatta gagatttctt agttactctc gaaacatact taattatagg
    14281 catcattata acaaaagctg catccataaa aactggaatt gaaattaaaa aacctgctgt
    14341 agttactgct aaagcactgt ttttttctcc agtcttagct aacaacccct ttgcaataga
    14401 ttctgttgct cttgattctg aaagaatatt tccaaatatt attccaagtc caattacaat
    14461 acctatacta cctaatgtat ttccaaatcc agtcgatatt gtggttgata tttcatttat
    14521 aggcattcta accataaagc ctgtaattat agatgttatc agtaatgcca aaaacggatt
    14581 tactttaaat ttaataatcg atacaagtaa aattcctata gatataacaa atgctatcaa
    14641 aagtccatat cctgtaatca taatctttac ccctttatca ttctacttta ttatttatca
    14701 attttatttt tcttttacta tgataactta acctcgctgt atttatataa tctaatccta
    14761 ataatatgta tttaattata ttgatatatt taaatattaa ttattgtgtt tttttatttc
    14821 aacacctgcc aacttttcaa aatactgtgc tattccacta tggtcatctt ccaaatgtcc
    14881 atgtactttt aatgtctgca taatttctaa taattgactt gtcataggta gcggtacatc
    14941 taaattgtgt gctgtttgca ttacattctt aatatctttc aaattaatag atatttttcc
    15001 acctggttta aaatcacgat tcataatcat tggtatcttc gcatccaata ttgtactacc
    15061 agctaggcct cctcttattg ccttatatac tttttctggg tctgcacctg cttttgctgc
    15121 aagtacaaat gcctcagaaa ctgctgctat cgtcaaatta actattactt ggtttgtaag
    15181 ttttgtaaca cttccactac cattatctcc aattaaaata gatgagcttc ccattatatt
    15241 aaaaaatggc tctactttct tataaatttc ttccttacca ccagccataa aagcaagtgt
    15301 accatcaatc gcttttggct cccctccact tactggtgag tctataaatt ctaagcccat
    15361 atcttttaat ttatttgcac acaatattga ttctgtagga gtaactgaac tcatatcgac
    15421 tactatactt ccttctttta aagtttttgc caatccatcc atcccaaata atatatcctg
    15481 aacaatagtt ccatttggaa ggattgtaaa tacaatatca cattcttgac ccatttctaa
    15541 aacacttgcg tattttgctc cacatgatat aagttcatcc acagcagatt tattaatgtc
    15601 atacaccaaa agattacatc catcttttaa taaatttttt gccattggct ttcccattat
    15661 tcctaatcca ataaatccca atttcatttt ataaccctcc atatcttcac atttttgaaa
    15721 acgtttttta tgttttagag tatactacat accagtatac cggtcaatat aaaatataaa
    15781 agtatttatt ttaacaataa atagtaattt ttaaagtcat ttattatatt tagtcatcct
    15841 atataagttt taatacatac tattgttttt aaatataata tgctactttt acagttttgt
    15901 atttttacta atttcaatta gttttaatta atctgccttt aatgttaatc atacttcaca
    15961 cttcaataat tttttatatt ttttaatctt atctgcaatt taaattgcaa aagattgacc
    16021 ttattttcac tatgttgtat gcttaaaatt atataagttt ttgtattttt atattgttta
    16081 ggggggtatt attaagtatg gctacattta aaagattaca agaacaagca tatgactact
    16141 taaaagaatt gatactatct ggagaaatga tagaaaatga aatatattct gaaacaaaac
    16201 ttgctagtga aataggtatc tctagaacac ctataagaga tgctcttcaa agacttagtc
    16261 aagatggatt tatagatata atcccaagca aaggatttag aatccatcaa attacagcaa
    16321 atgaaattgt agagatattc caaatacgct ctgcaataga agggttttgt acttttttaa
    16381 ttacaagtca atacaaagaa gctagggcag tagaaactat aagtaaatta aaacatcttt
    16441 tagataaaca aaaagatatt ttactaggtg ataaaaattt aaactcattt gcagaatacg
    16501 atacattatt tcacacaact atagtttcat atgctcaaaa tactgaattt gacaaaatgt
    16561 ttaacaacta tatgtacaga ataaaaaaat tagctctcga ttcattatca catgaaggaa
    16621 ggcttgaaac aacattaaaa gaacacacag atatttttaa taatattgca aatggatgta
    16681 ttgaagatgt ttacaaaact actctcattc atatggaaac tcctaaatac attaatttag
    16741 aagatttttg taacaataag tgattttagt attatacttt catttattaa aaatatatct
    16801 acctcaaatt aatttcctaa tttataaaaa atattctata attatattgt aactaatact
    16861 attctaagga gtgatttttt tgaacgaaaa tgttgtaact aatgaaacta ttgacaaaga
    16921 ttatgctatt gaagaagttc gtatggcatg caaacatttt ggtgacttat atttttattt
    16981 ttccaaagtg ttatttgaag agtttggtga agataaaact atcgaaatac taagaaaagt
    17041 tttatttgaa aggtctgaag aaagagctat tgctatgaga gaaagagctc ttgaaaatgg
    17101 agatgaatta attgcagaca atattattag cacaactgat gttccatttc taggatgggt
    17161 tcctgaattt aaagagttac attgccctta tggagcttcc tggcttttaa gatttgagga
    17221 aaacccttgg tttaaaaaat tcgcttctct ttactgtgat gttacagata ctactgtagc
    17281 tgaagtattt acaggagata cgtcccataa aataacaaaa aacattctat ggggagataa
    17341 aagttgtgaa agaatatatt tccatgatga caaggtatct gaaggtaaat atacttatgg
    17401 aaataaaaac tgtaaataaa taatttaaat tttctatatt catatactat attttgtact
    17461 ttttcatata aatattataa ctagtctttt attttacttt tgtttaacct aaagataatt
    17521 tacttttaat ctatatgaat tatcatataa ataatcagaa agttatcttt ataaattata
    17581 gttttagatt tataatttat aaaatattta tgttctttgt aaaatttttt agaagcttct
    17641 aatgttatgc tataaaacta tcttgagaag ggctgattga atgtttaata aaatatatga
    17701 aaagaaagaa tttatagttt ttcaagttaa aaaaggttat gtagtttata atactagaaa
    17761 gagttttgaa gaaggtcata cacatttaaa gcattttgaa gcagcaaaga cagctattga
    17821 tttagcaata aataaaaaga ttcctagaag caaagatgga tattatctga ctagtttaat
    17881 tcgcataagt gatgatggtt attacattga taagttaagc gaattgttat atgttagaga
    17941 acaaaaaggt aaaaaagaaa agtactgtaa ttctggttat cagatgtaac aagactagtt
    18001 gtgaattata agtaccaatc ataaatacct ttagtcttaa aatcaaagag tatcagtaaa
    18061 tctataaaaa aacaacttac tgatactctt tttaattata tgattaatat ttgaatttga
    18121 tacaaaattt tatacaaaag tttatgttat ctattctgat tctttgtctg caaattggct
    18181 attataaagc ttctcataaa atccaccctt ttccattaat tcatcatgat tcccctgctc
    18241 tataatactt ccattattca taactaaaat caagtcagca ttgcgaattg tagaaagtct
    18301 atgtgctata acaaaactag ttctaccatt cataagattt ctcattgctt tttgaagcat
    18361 aagttccagc ctagtatcaa cagaactagt agcttcatca agtatcaata ttgaagggtc
    18421 tgatataaaa gctctagcta tggttaaaag ctgtttttcc cctagagata tattagatgc
    18481 ttcttcattt aagaacatat tgtatccatc tggaagagtg gttataaagt gatgaacatt
    18541 tgctactttt gctgcttgta taatttcttc tttagtagct ccaaatctac catattctat
    18601 attttcaaaa atggttccat taaataacca tgtatcttga agcaccatac caaacattga
    18661 acgcaaatct ttacgtttca tatctcttat atctactcca tcaattttaa tagcaccttt
    18721 ctttacatca taaaacctca tcaataagtt tattaatgtt gttttacctg ctccagtagg
    18781 tccaacaata gctaccatct gaccactttt tacttctgca tttaaatctt ctattaaagt
    18841 tgaattttct ccatatccaa aattaacatg ctcaaatgta acatttcctt ttacattttc
    18901 tatcttaact ggattttcaa catcaggtat ttcctcttct tcgtctaaaa tttcaaatac
    18961 acgttctata gctgcaaaag aagattgtat tactgtagat aactgagtca tttgtgaaag
    19021 tggctgattt atctgccata tataacgcac aaatgcttgt aaatttccta ctgttatccc
    19081 tccacttact gcatatatag aaccgacaac tgccacagct gcaattccaa agtaagttac
    19141 taatgaaacc ataggattca tcatacttga tataaattgt gccttaaatc cattttcaca
    19201 aagttcttga tttacttttt taaattcttt tatagaatct tcttctttac catatagttt
    19261 tatttcatta aatccagtat acatttcttg tacttttcca tttaattttc caagagcatt
    19321 ttgttgcaat gaaaatctac tttgagattt ttttactaca aatttagata caaaataact
    19381 aattggaatt ataaaaagag ctataagagc catatatata ttaattgtaa acatcattga
    19441 aagtgccaaa attatgacta agattgagtt tacaatttgc ataaaactct gttgcaatgc
    19501 atttgatatt gtatctacat catttgatat acgacttaat acatctccaa aactattact
    19561 atcaaaataa ctaataggca atcttcttat tttattttcg acttcatttc ttaaatctct
    19621 cattgtgttt tgaatagcat tcgtaagtag aaaacttgaa gtatacgtaa aaattgcatt
    19681 agcaaaatat atacatgcta aaatcaccaa tatttttgtt acataatcaa aatttacgga
    19741 agcccctggt actccttttg cgatactaat tacatcttta gtcaattgtg ttattatcaa
    19801 tccttcagtt ttaggtgcaa gtgcattcat agtagctgct gaaattatac atataatagc
    19861 tataataaag gaaaatttat atttctttac aaatggaaga agtctaccaa ttgttttctt
    19921 aaaaccttta gactttttca ttattctaat tcctcctttt ttaactgaga tgttgcaatt
    19981 tcatagtata tttcacagtt ttttaataat tctttatgag ttcccattcc gacaacttgt
    20041 ccttcattga gtactataat cttatcagca tcaataatag aactaattct ctgggcaaca
    20101 atcaatacaa ttgcatcttt tgtttccttc ttcaatttag cacgaagtat tgcatccgtt
    20161 ttaaagtcaa gtgcagaaaa actatcatca aaaatgtata tttcaggtct tcttataata
    20221 gcccttgcaa tagaaagtct ttgtttttga cctcctgata catttgctcc accttcacta
    20281 atcaattcat caaactttct aggttttttt gatataaact catatgcttg tgcaactctt
    20341 gcggaatatt ctaattctga ctctcctgcc ttatgtttcc caaatctaat attatttgct
    20401 atgctaccag taaaaagcag tgatttttga ggtatgaatc ctatcttttt tcttaaagct
    20461 tttaaatcat attctcttat atctactcca tttatcttaa tgctaccctc tgttacatcg
    20521 taaaatctag gtatcaagtt aattagagta cttttcccac taccagtact tcctataaaa
    20581 gcgactgttt cacctgtttt tgcagtaaat gaaatatcct taagtacaga agcttctcca
    20641 tctggatagg taaaagttac attatcaaat tctatgatac ccttattttc agtatcctta
    20701 attccatttt caggattttt tatcaatggt tcttcattta aaagttcttt tattctattt
    20761 gcagatacct ctgctcttgg atacataata aataccattg aaaaaagcat aattgaaaac
    20821 atggcatgga aaagatattc caaaaatgca accaactgtc caacttgaag ctctccaata
    20881 tttatcttct cacttgaaat ccaaaataca gccaaaacag ctatatttaa tagtaaaaag
    20941 aatgctggtt gagtaattga cataagctta aatagtttct tagaaacatt agcataatat
    21001 gtatttgttt tttcaaatcg ctctgtttca taatcatcat ttccaaaagc tctaattact
    21061 cttattccag ttaaattttc tctcgatata aggtttaatt tatctagtcc tttttgttgt
    21121 tttgaagata ttgcctgaga tacttttgct attattgcta ctccaataat tataaaaggt
    21181 actgatatag ccaatactat agaaagttcc acacttgttc taattgtcat tattatactt
    21241 atgatgaaca taactggagt taataaggca gtccttagta gtatattaac aaattgttga
    21301 atttgaaatg catcattcgt tgttcttgta atcattgaag aaattccaaa tttattgtat
    21361 tcagtatgtg aaaattcttg tgatttctca aatatgtcat ttcttatatc tctggttaca
    21421 cttgttgata ccttagcaga acaataaccc aacaatatac tacctacaac ccctataata
    21481 gaaataataa caatcactat tcccatttgt tttatgtaag ctatattctg atttgcaata
    21541 ccattgtcaa ttattttagc tattatagtt ggaatcccca actcgactaa tgcaaaacta
    21601 aatatagcca gtatgtttaa tactattaat aacttataat tctttaaata acttaaaatt
    21661 aatttcatat ttttctcctt tcttaatttt tgagatagtg taatcatgta ataatactaa
    21721 atttataaat gcttaatttt ataaaaaaag gagaaaagag ttaacgcact ctcttctcca
    21781 gcatattctt tttaatacac cctccgacaa gtcgcttata tgttaacata tttgacaagt
    21841 aatgtcaatt catatagcta aaagtctttt aaatgcttca tctcataaca ttgtaacaaa
    21901 ttggcatgta atttattttt gttttttact gtattccaat tttacaagtt ctccaatatt
    21961 actaactttc ttaaaattca aattagtctt atctatacca gacttaaata gtgaaatccc
    22021 tctccccaat attattggca atatatatat ataataatca tcaattaagt tatctttcat
    22081 aaataatttt actatatcgc ctccaccaac tatccaaata tcttttttat ccttcttttt
    22141 tccaatttgc tcaattaatt ttttaggttc taaattagta tactttacat tctccagttg
    22201 tcccattaat tgtatttgag tatatatagc attccttacc cttatagtac cattcatttg
    22261 ggcttaattc atttgcaatc tgtacatatg tggaatttcc cataatgata gtatcaattg
    22321 tatcgtaaaa agtattataa tcatcttcct gctcattctt aaattctgaa gatatctcgt
    22381 ctaaaaaact tacactgcca ttctcttcag ctatataacc atctaaactt gtggcaatat
    22441 aaagtactac ttttctcatg tttactacct ccaacattta tagtgtgatt attctatgta
    22501 aacatcactt ttttactcat tcaataatac cctattaata aatcctcaaa ctaatttaaa
    22561 taaactttgc ataaaactaa aaatatatta tataaataat ttaaaatcac actaacttat
    22621 tgaaaattct aattattaca aataaatatt gctctctgtt aacgctagtt ttataatata
    22681 attataagct attaatatta taaagccgat attactaata taataattat agttaattta
    22741 cttttgttga aaggtggtaa ataacatgaa aatttcagaa gtcgctactc caaatttttt
    22801 gctagattta gaccagttag aaaagaatat agataaaatt caacaaattt gtacaaataa
    22861 taataaacaa ctttggccta tgcttaagac acataaaagt acttacatag cgagacttca
    22921 aaaaaatgca ggtgctagtg gttttctagt tggaacactt gatgaaggtg aagctctgat
    22981 aaaagcaggt tttgaagata ttatgttggc ttatccttac atgggagaag taaatataaa
    23041 tagaattgtg actatggcat caaaaataaa tttaatctgt gctacagata atatagaatg
    23101 tgctaaggct tatagtgaag ctttttctaa atcaaaaata gagtgcaagc tacttattat
    23161 tgtagattgt ggcttacacc gttttggggt tgaaccagaa aaagtggttg aacttgctac
    23221 tgaaatttct aagctagaaa acatcaagat tgttggtata agttcgcatc caggtcaagt
    23281 gtacggttgt agttctccag aaggtgttcc agatgtttgt gttcaagaag attcttctat
    23341 ggagaaagca tacaaagctc taactgataa tggatttaat atagaaattg tcgcttctgg
    23401 tagtactcca acagttgaag ctgaagctgc ttctaaaact ataactgctg taagacctgg
    23461 aaactatgta tactatgatg ctatacagat aggtttaggt tgtgctactg aagatatgtg
    23521 tgctcttact gttgtgtgta ctgtaatctc aaaaaatagc agtggatatt atttaattga
    23581 ttgtggaagt aagtgccttg gacttgataa aggtgcacat ggaaatagta gtattgttgg
    23641 ttatggtagg gtatgtggac atccagaact tactattgat tcactttcag aagaagttgg
    23701 aaaaattaaa gtaaatggtg aaactgatat aaaaattggt gatagaataa gaattatacc
    23761 taatcattct tgttccactg caaacttgac gagtaattta ttaggtttta gaggagatac
    23821 aatagaaaaa gtcatagaag tagatattcg tgaaaattca aaacaaatca tcctttaaaa
    23881 atatatttat tttgatatta acatcatgaa agtatatttt tcttaaaatt attgttaata
    23941 actaatgtta atgttggata aataaaaaac ataaattcta gaaatataaa tttaaaaaaa
    24001 gaggctgtct caaaattgga tatgtccctg tcaagtagac agacttaaaa aaggctatct
    24061 ttatgcggaa tgagttcgat attcaatcgg actcattcc
//
LOCUS       DAGQWR010000061        23223 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.061, whole genome shotgun sequence.
ACCESSION   DAGQWR010000061 DAGQWR010000000
VERSION     DAGQWR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23223
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.061"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            228..1460
                     /locus_tag="MIW95_002025"
     CDS             228..1460
                     /locus_tag="MIW95_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBY3151351.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            1474..2280
                     /locus_tag="MIW95_002026"
     CDS             1474..2280
                     /locus_tag="MIW95_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBY3151352.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKHGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2392..3261)
                     /locus_tag="MIW95_002027"
     CDS             complement(2392..3261)
                     /locus_tag="MIW95_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY3151353.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            3632..5059
                     /locus_tag="MIW95_002028"
     CDS             3632..5059
                     /locus_tag="MIW95_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBY3151354.1"
                     /translation="MKLTKSISSMLCLALVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFLNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEIFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(5135..5404)
                     /locus_tag="MIW95_002029"
     CDS             complement(5135..5404)
                     /locus_tag="MIW95_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151355.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGISINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5407..6849)
                     /gene="nhaC"
                     /locus_tag="MIW95_002030"
     CDS             complement(5407..6849)
                     /gene="nhaC"
                     /locus_tag="MIW95_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBY3151356.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAATKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDILNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(6874..8040)
                     /locus_tag="MIW95_002031"
     CDS             complement(6874..8040)
                     /locus_tag="MIW95_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY3151357.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESVLVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIK"
     gene            complement(8265..10385)
                     /locus_tag="MIW95_002032"
     CDS             complement(8265..10385)
                     /locus_tag="MIW95_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3151358.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVVDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHIARKILSINANEMIDSFVGDCIKDINFQNILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERAADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLGK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            10618..11019
                     /locus_tag="MIW95_002033"
     CDS             10618..11019
                     /locus_tag="MIW95_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151359.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            11062..11355
                     /locus_tag="MIW95_002034"
     CDS             11062..11355
                     /locus_tag="MIW95_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBY3151360.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            11370..11810
                     /locus_tag="MIW95_002035"
     CDS             11370..11810
                     /locus_tag="MIW95_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBY3151361.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(11902..12375)
                     /locus_tag="MIW95_002036"
     CDS             complement(11902..12375)
                     /locus_tag="MIW95_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBY3151362.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(12375..13163)
                     /locus_tag="MIW95_002037"
     CDS             complement(12375..13163)
                     /locus_tag="MIW95_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBY3151363.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            13491..14735
                     /locus_tag="MIW95_002038"
     CDS             13491..14735
                     /locus_tag="MIW95_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBY3151364.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGIHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLINKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            15058..16155
                     /locus_tag="MIW95_002039"
     CDS             15058..16155
                     /locus_tag="MIW95_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBY3151365.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEITIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            16146..16844
                     /locus_tag="MIW95_002040"
     CDS             16146..16844
                     /locus_tag="MIW95_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY3151366.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            16847..17911
                     /locus_tag="MIW95_002041"
     CDS             16847..17911
                     /locus_tag="MIW95_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBY3151367.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            18094..18798
                     /locus_tag="MIW95_002042"
     CDS             18094..18798
                     /locus_tag="MIW95_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3151368.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            18791..20113
                     /locus_tag="MIW95_002043"
     CDS             18791..20113
                     /locus_tag="MIW95_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3151369.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            20300..21673
                     /locus_tag="MIW95_002044"
     CDS             20300..21673
                     /locus_tag="MIW95_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151370.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIVMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(22046..22984)
                     /gene="pdaA"
                     /locus_tag="MIW95_002045"
     CDS             complement(22046..22984)
                     /gene="pdaA"
                     /locus_tag="MIW95_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBY3151371.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDGGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         8542 a         2901 c         3683 g         8097 t
ORIGIN      
        1 caaatttgaa ataagtctaa tatatgactt aatagattaa attaaaataa taaatttgga
       61 ctatttatct atttataact taaattatat caaattgata acaaactaga tattatactt
      121 ttttgtagaa tagaattaca attatataaa gaaaaattct attaatagtg aaaaaaaagt
      181 agaaaaaagt agaaattatt cttttataaa gaaagggagg gataatattg caagagagag
      241 aagaattagt tttaaatcca gttccaaaag aggaaagagt tggatggtta gctccattgt
      301 ttaatatgct tggttctaac atagctatat ctgaactaat ggttggagga actttaattt
      361 taggaatgac tctttctaat atgattatta catcaattat agggaactta atattagttg
      421 caatcattat gatacaaggt tatataggtt acaaagaagg gttaaatacg tatgtattag
      481 ctaagggggc atttggagag ataggaggaa agtatcttat atctctatta cttggtataa
      541 ctagttttgg atggtttggt gtacaagcag gggttgcagg actatcagta caaaagatat
      601 tcccaagtgt aaacctaaca ttagttacag ttatacttgg attatttatg gttgtttttg
      661 cactgtatgg ctttaaagct atggctaaat ttaattactt agttatacca ccacttatga
      721 tattaatggt gtggggagtt tttaaagctt tttctactta tggagttgaa gctatttata
      781 attatactcc tcaaacaact atgagtatgg ttgaaggtct taatattgta gtaggtcttg
      841 ttatagttgg tgcaattata tcacctgacc agttaagata tacgagacga gtaaaagata
      901 tttggattat cagtttttta ggtctaggga ttatttcatt attccaacaa gttgcagctg
      961 gtgtaatgtc tatgggagca cctacttggg atataacaga agtattggct aatttaggtt
     1021 ttggatgggt agcgtttgtt attttaatac ttgcatcttg gtcaacaaac gtatcaaatg
     1081 cttattctgg tggattagca ttaaaaacta tttttccaaa tgtaaaaaga aatgttttga
     1141 ctttagttgc aggtctaata ggtacaataa tagcagctac tggtataata tttaaatttc
     1201 agtcattttt atctttcttg ggaatcgcag ttcctgcaat agcaggaatt atgtggtgtg
     1261 agtattattt tatacaagga agaacttata agcatagaga aggaataaat tggattgcag
     1321 taataagctg gttaataggt tttgcagcgt cgtattatag ttcaaaaata aatttcttaa
     1381 tacctccgat aaatggaatt gtagtgagta tggttattta ttatatatta atgaaatgtt
     1441 ttggaatcaa agataaataa agggggcaat tacatgggtc gtaaggtgaa aataggtata
     1501 attcaacaac acagtgtact tggagatgtt aaaaaaaata tagaaaaagc agtagagatg
     1561 atagatgact taggaaaaca tggggcagat ataatttgtt taccagaact atttgctaca
     1621 ggatataatt tagagtcttt aggtggagtt aaaacattag aattaataag agaacataat
     1681 aagtacatag aggaatcaat gtcagaagct gctaaaagaa ataatgtata cctgatatct
     1741 ccatatggaa ccttagaaaa agggtctaca catgtttata attcagcagt tatatttgat
     1801 agaaaaggta agattatggg agaatactgt aaaaatcatc tgtggtcatt agaagcagta
     1861 tactttaaag gaggggaaaa agtagaggta tatgatgctg attttggtag atttggtgta
     1921 atgatatgtt atgatgcagg ttttccagaa gtatccagag aattgacatt aaaaggttct
     1981 gaaataatat ttataccatc tgcatggaga atacaagatg aagatatgtg ggatttaaat
     2041 gtatctcaaa gagctttaga gaacactgta tatacagtag gtgtaaattt agtaagtaat
     2101 gactctaatt taatactatt tggaaagagt aaaatatgca atcctagagg tactgtaata
     2161 actcaattag atacatacag ggaaaaatat gtattatcag agattgattt agatgaaata
     2221 aaaaagtata gggaagatat accctatttg aaagacagaa aaagtagcta taacatataa
     2281 ttctatacta taatacaagt tcaatataaa atgataaaaa gaggatgtat caatattaag
     2341 attaaattta ttatattttg atatatcctc tttaatatta tcaactttga tttaaatatt
     2401 tttaaaatag ttgataagaa agtttttgaa caacaacaca gctttagatt catagttttc
     2461 gctaaaccat gaaagattta aatatctttt aaattcaata tctttgatat gtaacaactt
     2521 aatctcaggg tcttgagata atccccatga ttttccacat ataaatccaa ctccttgaag
     2581 tgattttata agagcataaa ttgtatatgg tgaatcagac tcaaatgcta ttttgggttt
     2641 gaaattagct gattcgcaca ggatatcaat tacctctcta taattctcac ccttcgttat
     2701 aactataaag ttttcatctg aaacttcgct tagatatact tcatccttta atgataaagg
     2761 atggtttatt gaaactccta atagtatttc ttcgcatgtt aaagttatgc tattttctga
     2821 gtttagcttt gtaaatgaag aagatatgta caaatcaaaa tcactttttt tataagatga
     2881 tggcaaagta tgtgacacat taaaagtaat attgggatat agttttctga aattactcaa
     2941 aagtgatggt aacacattag ccgctgcagg agataatatc tttacttctc ctgaattttc
     3001 taaagaagtg tcttgtaact ccttttttga actttctact aaatctaaaa tgctatctac
     3061 tctttttaga aacaatcttc catactcatt taaaacaata tatcttcctt ttctgtcaaa
     3121 taactgaact ccaagttctt tttctaagga agatatagtt ttgcttaaag ctggctgtgc
     3181 tatatttaac ttgtgtgatg cttttgttat gtgctccatt ctagcaacag tttgaaaata
     3241 cttaagatgc aataaatcca tagtataaaa tacacctctt ttaatatgac ttgagttata
     3301 tcaattttat aatcaactcc acattataca ttataatata aagtttatat aatcaactac
     3361 aatatattaa aaagttattt aaattgttat ttatattttg tacataatct aattaaaaaa
     3421 agtagcttta aatgattaac taaggaaatg ttgttacaaa ctttagttat ataaaaaatt
     3481 catttgatga gtaaagtatt aatgtcaagt aagaataaaa gcttattaga tagaaaaata
     3541 atattttata ataatgatgt tacttaaatt attatgttat actattttaa ataggaaaca
     3601 tttagatact ataattttag ggggagtcaa gatgaaatta actaaatcaa tatcatcaat
     3661 gctatgctta gctctagttt taagcttatc accagtagca tatgcaaatg aaataagtag
     3721 aacaacagtg gagaatactt tagtaggtca ggacagatac caaactgctg ttgaagtaag
     3781 caaaaaagga tggagtagtg cgaatgaggc tgtagttgta aatagtgaat ctattgtaga
     3841 tgcattagct gtaacaccat ttgctaaatt aaaaaatgct cctatattat tgacagaaaa
     3901 aaataatcta aattcacaaa ctgagaaaga attaaaaaga ttaggagtta aaaaagttta
     3961 tataattggt ctatctagtt ctgtatcaaa tgatgtgcaa aaacagttag aatctaataa
     4021 tttaactact gatagagttg gtggtagtga tagataccaa acagctttga gtatagctaa
     4081 aaaaatagaa ggattaaaag atatatcaga aatagctgta gtaaatgggt acacaggtct
     4141 tgcagatgca gttagtatag cttctgtagc tgctactaat ggaatggtta tacttccagt
     4201 atctgatgca tctggtatat ctagctttaa agattttata gaatctaaga atataaataa
     4261 atcatacata ataggctcta ctaatgctgt atcagataaa ataaaacaaa gtttacctaa
     4321 ttcagaaaga ataggtggag cagatagaaa tgaaactaat ggtaaagtaa tagaaaagtt
     4381 ttatacatct aataaattaa acaatgtatt tgtggctaag gatggaatga aaaatcaaaa
     4441 tcaacttata gacgctctag cagtaggagt actggcatca aaagaaaatt ctcctgtact
     4501 aatcgtagga gaacaattaa gtgcaacaca aaagagtgta tttttgaata aaaatgctac
     4561 ctctataact caagttggta gtggaggaaa tgaaaatgca tttaaagaat taaaagattt
     4621 acaggcaaat agtaacaata agagagtaat gtatgtaact aatacagata aggtaaatat
     4681 aagaaatgat gctacaatag aagcttctgt tataggatat ctaaataatg gtgatgaagt
     4741 agaagtttta gatgtgttaa aaactggatg ggtaaaaata aaatacaatg aaggtattgg
     4801 atatgttagt gggtcttatt tgacaaataa taagcctgat aatagtaatg aaaatataaa
     4861 aataaagtat gtaaaagaaa aagatggtct aaatgttagg aaaggaccaa gtacagaaga
     4921 tgaaaaaata ggtcaccttt catatggaag caaagtagaa acaatagaaa tatttgctac
     4981 tggatgggta aaaataaagt ataatggtgg atatggttat gtaagtaatg attacttatc
     5041 tgatacacca gttatttaat tataagtaac aataaaaaac tgctttaatt tgtaaagcag
     5101 tttttttata aaaatttaag tctaataact atttctattt tgatacctta atcctgggat
     5161 ttattattat ggatattatc aacatacaaa cactccaaat ccagtgtcta attccaaact
     5221 tagatattaa taaattcagt cctgctatgg taaataagca gtatttaatt acaattaaaa
     5281 gtgtatttat agaaatcccc ttcatagaaa gtatcctaaa tccaataata gacattctaa
     5341 gtcctataaa tgccaataat atacctgcaa tatcctgaat taagtaatag tattcaaatt
     5401 tcatattcaa tcaactcttc tctgagcagt atcaaatatt tctaactctt caatttcatc
     5461 atctgtgtat gtaagcatag taaaaccagt tacaccataa ataaatgaaa ttataggtgt
     5521 aatccaactt aagaaacaat atggaataaa ttctagtggg cttacaccaa gagtctgagc
     5581 acagaaaaca gcatttgaat tccatggtat taatggacca ccaagtgtag ctgtatcttc
     5641 tataattcta gatagatttt gtggttttaa tttaaactct ttgtatattg gagacattag
     5701 agttccgaca aatacagaag taaacatcat tgatgaacta aatgcatttg caaagtagct
     5761 tactataaaa gtagacccaa ccaatttagt tcttgatttt cctattttag ttataagagg
     5821 ttgaagcagt acttctaaga atccagtttt ttggagaata ccggcaataa cgaaaactac
     5881 aaatactagt aaaacggttt cagccatact cattattcca ccacgattta atagtttgtc
     5941 ggcatagaca aatccagtat ctatagtaaa tcctgatagc atgtaattta aaatatcacc
     6001 tattttttct ccttcttgaa atactgctat aataagtccc atgatagctg aacttagtat
     6061 agatataatg ggtggctttt gtagcaataa aagtaataat aaaaatatta ttggaatcat
     6121 tgctactaaa ccaatatgaa agtttgaatt taatacatcc ttgatttgat ttatttgaac
     6181 atagtctata gtattatttg aatacttaaa tccaattact gtatacagaa ctatacatat
     6241 tacgtatgca ggtcctgtgg tataaaacat atgcttcata tgtgtaatta catctgtctt
     6301 acaaactgct gcagctaagt ttgtagaatc tgatagaggt gacatcttat ctccaaagaa
     6361 agcaccagaa atgactgcac cagctgttaa accaagagga atacccatac tagtaccaat
     6421 actcatcatt gctataccag aagttccaag agtcccccaa gatgtaccag tagcaacaga
     6481 agttaatgaa catacgatta atgttgtaag cagaaaatat ttaggggtta ttattgttag
     6541 tccactatat actactgttg gtattgtacc agcagctatc caagctgcta ttagtacacc
     6601 aacagaaagt ataattaagt ttgattgaaa ggcatcttta ccaacttcaa atccaaaagc
     6661 ttctatttca tcattagtat aaccaagttt cgtggctgca ggaactacta taagccatga
     6721 aagtaaaaac attgtagtta tagaagcatt aaatacaact ataccaactg atgttagagc
     6781 tattattgca agcattacta acaaggaata tctaaatgaa ggtatttttc tttctgtatt
     6841 cattttcata gtgcaaattc tccttaaata tttttattta attgcatttt ttattcttat
     6901 aagagcttct tcaagcatac ttcttggaca agctaagttt attctttgat acccacttcc
     6961 tccaatgcca aagctgtttc cttgatttag agcaacttta cctttttgta caagtacact
     7021 ttctagttcc tcatcactta atccaagtgc actaaagtct acccataata aataagtacc
     7081 ttctggtttt ctaactttaa gttttggcat attttcattt atatatttta tagcaaaatc
     7141 tatattagat tctaaatatt ctaaaaatga ttctagccaa gattcaccat tattatatga
     7201 agcttcagtt gcaactaagc taaagcaatt atttctcttt atatcaattc tggtaaaagc
     7261 atcatctaac aatttatagt ctttttcatc aggtagtatt acatatgatg attgaagacc
     7321 agctatatta aaagttttag ttggagccat acaagtaatt gtattttttt caaattcttt
     7381 agaaatagat gccataggaa tatgcttatg tttttttaat ataatgtcac tatgtatttc
     7441 atcagatatg atttttacat tatgcttaag acaaatatct cctagttttt ttaattcatc
     7501 ttttgtccat actctaccaa ctggattatg aggattacaa agtatgaata attttacatc
     7561 tttaatcttg ttttcaatgt cttcataatc cattatatag tttccatttt ctagtttctg
     7621 taatgggctt ataattagtt ctctattgtt attttttaca acactattaa aagggctgta
     7681 aactggttct tgtatcatta ttttatcatt tgcttttgtg agttcattta ttaaaagact
     7741 tatagctggg ataacacctg gactataaat taaccactca ctttttattt tccagttatg
     7801 tctcctatct agccagttta ctatagattc attgtaagaa tctggtcttg ttgtgtagcc
     7861 ataaatctct tgttctagtc tattttttaa ggaatcaatt atacaaggtg cagccttaaa
     7921 atccatgtca gccacccaca taggaagtaa atcattagtt ccatactttt tttccatttc
     7981 tgaccattta gatgaaaaat tattgcttct atcaacaatc tcattaaaat tataattcat
     8041 tgtaaatacc tccagtaatt ttaattttat gttaataata ttaagcaaac aacgtgccaa
     8101 tattgataat aaaaaatatt tattcacaag ttaatattta atattaagta atttcagagc
     8161 atagataaga taaaaatatt aattatactt aaaactttgt ggaaaaggtt atttctaata
     8221 aatttttaaa tatatgaaaa tgtaattaaa taggttaaat aggtttaact gtttaacttg
     8281 ttagacctat ttatattttt tagaaattct actccttttg atgttataat tgaacctcca
     8341 cgaccaacaa gtatgtctac aagcccaaag tcttgtagtt ctaaaagcat atttctaatc
     8401 tgttgttctg tcaaaaatct attttcctct aaagctattt tacataagct ttttctacct
     8461 attctttctt ttaaatcata agcctttttt aaattatcca atataaatat gtattcatct
     8521 agtgaccttt taaaattata gtcgtatttc atgatgtttt tgtctttgcc aatgttgaat
     8581 tgatactttt ttatattatc acttacttta ttacaaacgg ttctactata atttgaatct
     8641 atagtgtcca atatatattc aggtaaatca catatttcta taatatcttt tcccaagtaa
     8701 cagaagtatt ctcctaagtt tcttaattct cttacatttc cttcccatct atacatttta
     8761 aagatttctt ttacttcctc acttagaata aaattacatg gaatatcata ttttaatgaa
     8821 ccaaaaattt caaatatatc agcagctctt tccctaagtg gatgtatttt aaggggaaga
     8881 acattcaacc taaaatataa atctcttcta aacatatttt tagatacaag ttcttttaaa
     8941 tttctattag tagctgcaat tattcttaca tcaatatcaa tgacattatt agaaccaata
     9001 cgtcgtactt gtttttcttg tatgactcta agtaatttag attgagaatt taaatccatt
     9061 tctccaattt cgtctaaaaa tatagttcca ttgttggcta attcaaataa accaattttc
     9121 ccaccttttt tagcaccagt aaaagcccct tcatcatagc caaataattc actttgcagt
     9181 agattttctt gaaaagtact acaatttact gcgacaaagg gaccatcttt tctcctagaa
     9241 gcactatgaa tggattgagc aaataattct ttaccagtac cactttcacc aattataagg
     9301 atagatgaat ttgattgagc cattttatta gcaatttttt ttgtattaat agtttgtata
     9361 ctagaaccta atatatcatc aaatgtgtat ttactgatat taccagagtt tactagttgt
     9421 gctcttaatt tagcttgctt tttttctaat tgagaaaact ttgtcatctt tagtataaat
     9481 ccatcaaata catttaattg tatatgttta atctctaaat ttatatcaat atgatttatt
     9541 ttaatcaatt tctcaaaaaa tggagtttta ttggtcaata tgttttgaaa attgatgtct
     9601 tttatacaat caccaacaaa tgagtcaatc atttcatttg catttataga caaaattttt
     9661 ctagctatat gattatagaa ttgaattatt ccatcattat ttgtgcatat aattccatca
     9721 tcaattatag atagtaacaa atcaaattgt ctttcaaact tatttgcatc taacaataac
     9781 tcacctgtac tataacttgt aggaattatt ttttctacgt atttttttat taggtcatct
     9841 tttattagat gttttaattt ggtttttgta gcaatttcaa caatacaact taaatctatt
     9901 attctgtatt ttaaatctac aacttcacaa tttttaaact taggcaggat tttttcgcca
     9961 ggagttagga ttacagcatc tgtgggagta aactcaatct ctggatagca gggcaggata
    10021 tttatattgt ctattcctaa tctatgtatt aaagaaattg tttctattgc catatcttta
    10081 cttaagttat atacataaat aattttatct ttttctaatt tggatatcat atcaatactg
    10141 ttcttttcaa atgtcaactt gggaacaatg atttgagcat catagtgaga taattttact
    10201 atttcatcgt attttaaata agcagatagg agtataagtt tttctttaaa aaactttaaa
    10261 tttccctctt caaatgagta taaatttatc tttagattat cacctaagaa ttgccttatt
    10321 tgattttcat aaatttctcc agcgtctttt tttaaactta caatagctaa ttccatttgt
    10381 ttcattttaa cctctcatac aaatttgact tattttagat tacataataa ttaaattaga
    10441 ttaaataata ttatttaatt attattataa atttattata gactacacat tgaagtaaaa
    10501 gaagtagcta aaattattcc ttttattttt gttttttgta tctatttggt ataatagtat
    10561 tagagcgtat aataatttag gcaaaatttt ttaaggaaga ctagaggaga atataaaatg
    10621 aaaaaatgtc tttactgtaa tagggatata aagtcaccaa ataataaatt agctataaaa
    10681 tatgatgaca atatgaatat atatccatgt cgtgcagaat gtaaggaaaa aattgaagaa
    10741 tatatagcaa aaaaacctac atataagttt ataaatatat tagaggttct tttaggtgtg
    10801 ggcggaatat tctgcttttt gagtaaatgg actattgcag cccaagttgt acttggaatt
    10861 gatgcactgg ttatattttt attcccattg gcaggattta agatgaatgg aaagttaagt
    10921 atggaacaaa ctaagaatca aagtaggtct attgcaatat caattttggc atttatagta
    10981 ataataaccg tattatattt taaacaagtt ggtaagtaga gcataattgt ataagacatc
    11041 attagagtat gaggtgagtt tatggaagaa agaaaattag caccaaacaa tgagagggaa
    11101 gctctcataa aaagattaaa aagaatagaa ggtcaagtta aggggataca aaagatggtg
    11161 gaacaagaac gttattgtgt tgatatattg gttcaaatat ctgctataag gtctgctata
    11221 aatagagtag gcacaattat cttagagaat catataaaag gatgtgtagc agaaagcatt
    11281 aaacatgatg atgctgataa aactgaagag atgataaaag agcttatgga tactataaat
    11341 aaatttacaa aataaggagg cggttagtta tgaatcaaca aggatttatt atggaaattg
    11401 ttgatgatag aactgcaaag ttaaaaatgc aaagacattc agcttgtgct gcttgtggaa
    11461 aatgtgctac tacaaattca gcagagtcaa aagaaatatt agtagaagta gacaacacaa
    11521 ttggtgcaaa agttggtgac catgtagaag tgagtatgga taatatgaat gtattaaaag
    11581 cagctgtaat ggcttatata atacctctta tagcacttct tgtaggaaca atagggactt
    11641 attatatatt gggggcaatt ggaatgacaa ctggagttga agccataagt ggattagttg
    11701 gaatttgtct tacaataatc tgttatttat acttaaagaa gaatgacaaa aaatttagag
    11761 acagtagaga atttatacca gtaataacaa gaatattaat tgacctttaa gataattata
    11821 attaaaaaat agcaatgtgc tttttaaata aatactcaat acgtatttgt agagtttaag
    11881 tacattgcta tttttatatt tttattttga ttttctgtaa ggaataaagt tttttctgat
    11941 tttcaaaaga ttcaatgttc ttacaaaaga agttacaact aatacaccaa gtgctattgc
    12001 agcacttaga aatagcgcat ctgtaccttt tgacatagcc aaagaataat ttttctgaat
    12061 caaagacaac attgtattat atagcccaat tcctggtatt agaggaagaa tacctggaat
    12121 tacgaacaca atagctggtt gctttagttt tcttgctaaa gtctcactac acgctgagac
    12181 caaagcagca gcaataaaac ctgcaaaaac tgcatttgta gtaaaattaa ataaataaac
    12241 atatacaatc caaccaattc cgccagttac tccagctgga agaagtagat atagtggagc
    12301 attaaagaaa acagcaaatc cagcagttgc aacacctgaa aataaaaaat gcaaaatcat
    12361 agacatatca gtcattagaa aggacctcct attctatacc atgtatcaag gacaaaacca
    12421 actccagaag caattgcaat aataatcata gcagcatcaa aagcacgaga aattcctgat
    12481 attaaattcc ctgatattaa atctctaata gcttttataa aagcaactcc tgggagtagt
    12541 ggcattatag cgccgactat aagcatttta gggtcatcta aaatgcctat ttcaactagt
    12601 agaactcctg aaagtgcaat taaaaaagaa gatactaaac aggaaaatgc agatattgca
    12661 cttatcttca tcgttttatc ataagtaatt gcagcaaaga ttgctattat aaacgtaagt
    12721 ataaaattta atacataatt ccctccaagc aaacaagcaa aacaagcaga tgcaattcct
    12781 gtacaagagt aaaataacca taaaggataa ggttttacat cttggatttc ataaagacgt
    12841 gcaatggcat tatcaatatc atactctttg tcactaacaa attctcgaga aaaactgttt
    12901 aataaagaaa tcttgtttaa gtttatacct cttgattgaa tcgttttcat aaacgaaaag
    12961 ccatcaaatt tctcatctga aataattatt actgtaggag ttgtaaaaat atttacatga
    13021 taaaaccctc ttgacctgca tattctaagg acactgtctt caaccctaga tgtttcagct
    13081 ccatttttta tcatcagttc ccctacaaat agagctagtt ttagaatatt tttcttatat
    13141 tcaagttcct cattttgatt cataaataca cctccaaatt aaaaaacgta atattgaaat
    13201 tatatcaaat ttttaagtaa tattccttaa tatctatata aatatattat cttttttttg
    13261 tttcaaaata gtcacattta aattatatcc aattaaaaga attagacaat attatttatt
    13321 atattttaac ttaaaaataa agaaagtaac tatgttaaat atggaaaaat ggcaaaacac
    13381 ttgactatta attggtaaag gactataata ataaattatc atattgataa aaacgaaaat
    13441 tatgaaatgt ctgtaaaaat aaaatttaaa taataactag gaggagtaat atgtttgaga
    13501 acttgaaaaa agcggatcct gagatttatg aaagtatgaa aagagagtta aaaagacaac
    13561 aaagaaatat tgaattaata gcatctgaaa acatagtatc agttccagta atggaaacta
    13621 tgggaagtca tcttacaaat aagtatgcag aaggttactc aggcaaaaga tattatggtg
    13681 gatgtgaata catagatgaa gttgaaactc ttgcaataga tagaatcaaa aaaatatttg
    13741 gagcagaaca tgctaatgtt caacctcact ctggagcaaa tgcaaatatt ggtgtatatt
    13801 ttgctatgtt aaaaccaggt gatgttgtta tgggaatgaa tttatctcaa ggtggacatt
    13861 taactcatgg tgctccagtt aatatttctg gtcaatatta taaattttat gaatatggta
    13921 tagataaaga cagtggactt atagattttg atgaagttag aaagatagca catgaagtaa
    13981 aacctaaaat gatagttgca ggagcaagtg catatcctag agaaatagat tttaaaaagt
    14041 ttagagagat agctgatgag gttggagcat tgcttatggt agatatggct catatagcag
    14101 gtcttgtggc tgctggtatt caccaaaatc catgtgaagt ggctgatttt gttacaacaa
    14161 caactcacaa aactttaaga ggacctcgtg ggggtgttat tctttgtaaa gaaaaatatg
    14221 ctaaagacat agacaaagca atattccctg gtattcaagg tggacctctt gaacatataa
    14281 ttgcttctaa ggcagtttgc tttaaagaag ctttaagtga tgagtttaaa gaatatcagg
    14341 tacaagttgc taaaaatgca aaagccttag ctgaagaact tattaaaaga gattttaaat
    14401 tgatttctgg aggtacagat aatcatttaa tactattaga tttgattaat aaaaatataa
    14461 ctggtaaagc agcagaaaag agattggatg atgcttatat aacagctaat aaaaatacaa
    14521 ttccatttga tccaaatggt gctcttgtta caagtggaat aagacttggt actccagctg
    14581 taactactag aggtatgaaa gaagaagata tggcaataat cgctgaggct atagacctat
    14641 gtttaactta tgatgaagaa tctaaggcta gaacattggt tgttggttta actgaaaaat
    14701 atcctttata tgaagaatat aatattatgg attaattata aagaacaact aaataaaata
    14761 catttaaata attacaatga ttgtgtatgt atttgaatat atttagatat aaaaaaggtg
    14821 tctcaaaatg aactttttag tttatgaggc acttttttgt atggaatcga acatatttgt
    14881 gttatttaaa taataaaaaa tatggttatc tctattttta gatggtctct ttgtattttt
    14941 taattaaaac tttaagattt cttaagaatt atactaggtt tttctaaaga tttaaagtct
    15001 atacttataa gtatagtaat catatgaaaa gaaaagcttt aacgaggagg gaatcttatg
    15061 aaggtattaa ttcttacagg aaaatttggt atgggtcatt actcagcatc aaattcttta
    15121 agtgaagata taaaagcaaa atttgataat tcagaaataa caattaaaga tatatttgaa
    15181 tacattatgc ctaactattc agataaaatg tacaagactt tttctattct tgtaaatcgg
    15241 ggaagtagtc tttataattt gttttataaa tgtgctgaaa atggaaagaa agatataaaa
    15301 tttacttttt cagattattt tttaaataaa ttagatacat tacttcatga agtacaacca
    15361 acagtagtta tttcaacttt tccattttgt tctcaattag tatcaagata taaggaaaaa
    15421 tataactcaa atcttccttt aataacatgc ataactgata taagtagtca ctcagaatgg
    15481 attagtaaaa atacagactg ttatttagta gctagtaaat ctactaagga agaacttgtt
    15541 tttaaaggaa tagatgaatc taagattaaa gttaatggta ttccagtaaa aaaagaattt
    15601 aaaagaatag aacatgtaaa tcattcaact aaaaaaaata ttcttataat gggtggagga
    15661 ttaggattac tgcctaaatc agaacaattt tacaaagaat taaatagttt agaaggagta
    15721 aaaactactg taataactgg taataataaa aaaatgtact ataaactata tggtagatat
    15781 gaaaatatag aggtagtagg ttacaccaat gaagtatata aatatatgaa agattctgac
    15841 cttattattt ctaaaccagg tggaataaca ctatttgaaa caatttattc agaacttcca
    15901 atattggcat ttaatccatt tttacaacaa gagatagata atgctagttt tatattaaat
    15961 aatgaaattg ggagaatact tggtaaaaat aaaaaatact atgtagatga gataaaagat
    16021 ttaatttatg atgatgctac tcttaaagaa atgagcagta acatgaaaga attaaaaaaa
    16081 caatttgaca ataatacctt agagaatatt ttattttcac ttgatgaaca aggagcgtgt
    16141 agagaatgta tgtagtaggt ttaattatcg ttgttgcatt gattttttta gtccattcaa
    16201 ttatacctac atactataat aaactattaa ataaagaggt attaaaaaat atggctggag
    16261 aaaatgaaat tgcacttaca tttgatgatg gtccagataa gagatataca gaaaaattac
    16321 tagatgtgtt aaaagaaaat gatatacaag ctatgttttt tgtagtagca aagaatgctg
    16381 aaaaagagcc tgaaattata aaaagaatgt tgagagaaaa tcatatagtt ggtttacatt
    16441 cattggaaca cagaaatgca tggctttata gttatagtta tgttaaaaaa gattttattg
    16501 aaagtacaaa tataatgaaa aatttaggtg tagatgtaaa ctactatcgt ccaccatggg
    16561 gtcatactaa tatattttca aattcatttg ttaaaaaata taatctaaaa atgactttgt
    16621 gggatgtaat ggcagaagat tgggagaaag atagtacagt agacattatt ataaacaagt
    16681 taatgagtag aacaaaagaa aattctataa tttgtttaca tgatgcaggt gaaaattcag
    16741 gtggtgcagt aggtgcacct gagcgtacta ttgaagcttt aaagatagct attccaaaac
    16801 ttaaagccag tggtttaaag ttcgtaacac cagaaaggat gtagttatga taagtaaaac
    16861 tgaaaagaaa aataaatgcc taggtagtgt agctttttta gttttactaa tgggcataac
    16921 aggatatttt gtttttagag ggcagtcagt tgaatcattg ataaaatcat taaaaggtgc
    16981 aagccctatg tttatactaa ttggatttgc aatgatgttt atatatgttg cctgtgaagg
    17041 tataaatata tacttaggta tgaaagcttt aaaccaaaaa actactcttt taaaatgtat
    17101 gggatatgct tttattggat tttattttag ttctattacg ccttcagcat caggtggtca
    17161 accagcacaa gtttattata tgaaaaaaga cgatataaat atatcatatt cttcacttat
    17221 actattagtc attgttgtta ttcaccaagt agttatactg gcatatagtg gaataatgtt
    17281 cattatggaa cgtgagttta tattaaataa tgtaagtggt atgaacattt tactaatata
    17341 tggtgtaata acaaatgttg ctcttgtcat tggagtaatt gcaattatat tttcaaaaaa
    17401 acttgtaaac aattttataa tatcaataac aaatttatta ggtaagttaa gaataataaa
    17461 agatgttgaa agttcaagaa aagttataat atctcaaatt gaagagtatg ttaaaggtgc
    17521 tcaatatata aagcagaatc caaaacttgt agtccaaatt ttagtcataa ctattgtaca
    17581 aataacagct atgtttttag ttcctttctt tgtatacaaa gcttttcatc tatcaacata
    17641 tactgtattt gagatattag cgatacaatc cttgcttaat attgctgttt cttcattacc
    17701 attgccaggg gctgttggag cttctgaaaa tagttttatg acattattta agatattttt
    17761 ccctggtcac ttattagttc cggcaatgtt acttagtagg ggaataagtt tttatgcatt
    17821 tgtagctata agtggattga tatgtatagt agtacatgta aaatcttctg aaaaagtaaa
    17881 agcagtgaaa aaagttgctt atgtaagata atattatata gattattttt attaatatgt
    17941 caatttgtgt gtacatttta attaagtttt aaacatgttt acaggagtat aaactattaa
    18001 aacgtgtgac attgctaagc ttttgctaat aaagtataat attttatata gtttgaaata
    18061 tttaagaaat atagcaaaaa aaggagcttg gtgatgaata aagagattcc aaaaaaaatt
    18121 ttagtagctg atgataatcc tgagattagg gaaatcgtag aaatactttt aactggtgaa
    18181 ggttatgaag ttattatggc aacgaatgga gaagaagctg taaacttagt agatagtacg
    18241 attgatttga ttattttgga tgttgtaatg cctgtaaaaa caggatttgt tgcatgtaat
    18301 gaaattagag aaaaaacgac agcaccaata ttatttttaa ctgcaaaaac tcaagactct
    18361 gataaagtta ttggattttc agcaggagga gatgactatt tatcaaagcc tttttcatat
    18421 tctgaactaa tttcaagggt taagtcgcta cttagacgtt attatatata ccagggaaag
    18481 gaaaaggaaa gagagccaaa tatattaaaa acaaaagagt tatgtgtcaa tttagatact
    18541 caagatgtta cactaaatgg agaacctgtg tttttgacaa ctatagaata tagtatatta
    18601 attcttatgt tgaaaaatag aaaaaaagtt ttttcgtctg aaaatttata tgaaactatt
    18661 tgggatgagc agtattttta tacggcaaat aatacaataa tggttcatat tagaaattta
    18721 agaaaaaagt tagaaaaaga ccaaaaaaat ccacaatata taaaaaccgc atggggaaag
    18781 gggtattata ttgattaaca aaattaagcc atctaagttg ggagttatac tattgatata
    18841 tgtagtaatc tcagctgcag tggctttcat tttaaatata ccattttact ctgctgttga
    18901 atctatagta aaagatgtat cagaagggaa agtatatttt aaaagagaag taaatcaact
    18961 tatggataat tttcaaactt atgtgaataa caatggtata agagcagatg atggagatga
    19021 tatagcaaaa tggaatagtg ataattggtt tgtttctatg aagatatata aaggaaataa
    19081 ggttttttat gatacattat actatcctaa tgaagtacct gataagagtg aggaaaatga
    19141 gttttatagc agtcccatgg ctagaaacta tgagatacaa ttttctgata ttaaagctaa
    19201 tatagttgta gtagctttat ttagatttag atttcaaaat tttatagaag caatttctat
    19261 tacatttatg gcacttacat ttattataac atttttactg ttgtttagta gaaagataaa
    19321 gtacctaagg aaaatagaaa tgggtataaa gatagtagag agtggcagtt taaagtatag
    19381 aatcccagta aaaggtaatg atgaattatc atcacttgca tctagtatta atgagatgag
    19441 tcatacattg gagaatcaac ttataaaaga aagggagatg aagaataaaa ataaagatat
    19501 agtaacttcg atatcacatg atgttagaac tccattaaca tcagtaattt gttatttaga
    19561 tttgatcaaa gaccataaat acaatagtcc tgagcaattg gaacaataca taaaaaatgt
    19621 aaggtctaaa gcatatcaaa taaaagattt aacagatgac ctttttacgc attttgttaa
    19681 ttctggagag gaagtttcaa aagggtatga aaaaataaat gctaatgaat ttatttttca
    19741 attactttta gatagttcat atacattgga ggagaaagga tttcatataa caatagaaaa
    19801 tgaactttat aaccaatatc atatttatgt agatgttaca caatttagaa ggatatttga
    19861 taatttatgt tctaatataa taaaatatgc taggaaagat aagcctatag tatttttaat
    19921 taaagtagag gattctaaat tactaattat acaaaaaaat agagtaagta atttttctaa
    19981 gaatgtagaa agttttggaa ttggtataaa gaattgtgag aaaatagttg catatcataa
    20041 tggaagcata aatgtaaatt cagatgataa agaatttgct attgaaatat ctattccagc
    20101 aaaagaatca taaaaattta aattatatat tgtgtaattg ctattatata ttgtgtaatt
    20161 gcttatgtaa aattctatga agcaggaagc atgtgacttt agttatggga tgttcaatat
    20221 tgtgtacctc taggcaagta acaagtttac ttacaagacc atataatatt attatcttaa
    20281 aaaaagggga cataataata tggttttgac aggagcacat tatatttaca ttctatttat
    20341 ggtaattata ttaattacta tgattatgaa aaaggataca atagtaccat gtatattagg
    20401 tgtttttttt atgggattgt tctttgaaaa aaacatcttt ggtgctataa cagcagtgtt
    20461 taactctttt attatatctt taaatgagtt gggtccaatt atcttgatta tagctattat
    20521 ggttgcatta tctaaagccc tagaagccaa taatgctata cagtatatgg tgagaccatt
    20581 ttcgagagtt ataaaaaatt ctaatacagc attttttgta actggatttg ttatgttagt
    20641 tctttcttgg tttttctggc caactccagc tgttgcctta gttggagctg tatttttacc
    20701 tgttgctatg agagctgggc taccagcaat tggtgttgca gtagcattaa atttatttgg
    20761 tcatggtctt gccttgtcta cagactttgt tattcaagga gctccaagta taacagctgg
    20821 tgcagcaggt gttgcagtat catatgttat aaatgatggt atgattttgt tttgggtgat
    20881 gggaatcgta accatatcta tggcatttta tacattaaaa agagatatta acaaaggaat
    20941 gtttagagaa gaactaaaag gttttgaaag tgaagaagta gaagagttca atggaaaatc
    21001 taaaatagca actattttag tagctctggg atttcttgct gatataattg ttatgtatat
    21061 atttgaccta aaaggaggag atgcatcagc tcttttgggt ggaacagcag tatttttaat
    21121 aataattata aatgttataa attttggcaa agatagtctt gaaaatgtat gtgagaatat
    21181 aattgatggt tttgtgtttg ggataaaaat atttggagca attataccaa tagcagcatt
    21241 tttctatatg ggtgaagttg cgcccttgac aggtgtattt ggaaaagtat tagcaccagg
    21301 ttcacaaggt ttattatcag atataggcat agctctttct caaacaatac cacttaataa
    21361 atttgccgta tcaggtatag aaactgtagt gggggcaata actgggcttg atggttctgg
    21421 attttctggg atttcattag ttggttcact tgcctctgta tttggaactg ctataaatgc
    21481 aagtgttggt gcattagctg cattaggaca aatcagtggt atatgggtag gtggaggctg
    21541 tctagttcct tggggtctta tttcagcagc ggcaatttgt ggggttagtc caatagagtt
    21601 ggcaaagcga aactttgtcc cagttataac cggattggtt gtaacaacca tagtagcaat
    21661 gtttattatt taataaatcg aataaatctt taattgtaca aattaaaaat agatgtgtag
    21721 ctataattta ttataattgt tttactaatt ttaagccata catctatttt tattgatatt
    21781 tttattagta ttctttaata aaataatatt tttcattata ctatacataa catctaaata
    21841 taacatctaa aatactgtta tctatgttta atatcttgcc aaattttttg atgttttatt
    21901 accctgtccc accaacatat ataagctatc gcaagttttt tacctatact atcaccaaac
    21961 aaaaatttta attttaattt attattttat aatctatatt atattaacta tattagtttg
    22021 tagactttag atgtaattct aaatattaat actccaagag ttcaaattca tatcctagtt
    22081 ttcttgcatt tgatataaaa tcatttaaaa tttctgtact agttttggaa actgcatgta
    22141 gtaacaagat agaaccatca tgtagattat ccattatttt ttgcgttgct tcttcatgtg
    22201 atggttgttt atcagtatcc cagtctctgt atgcaaaact ccaaaataca gtcttgtatc
    22261 ctaaattttt agtcattgcc aagctttttt ctgagtattt tcccatagga ggtctaaaaa
    22321 atttaaccat atctttccca gttacatctt tatataactt ttcaacatca tataattcgt
    22381 cattaaagtt ttttagattg cttgtttttg ttggcataga tggatgggtt ttactatgat
    22441 ttccaactat atgtccctca gaaaccattc tttttactaa atctttattt tcttttatat
    22501 atggagctgt aacaaaaaaa actgctttaa cctggttttg ttttagagtg tcgagtatct
    22561 ttgtagtgta tccattttca taaccttcat caaatgtaag atatagtgtt ttttgaccat
    22621 cttttgtagg tccattgtat agggcatcat agtcagaaaa tttaaattca atgtccgtat
    22681 ttacttctgg tgttttatgc ttattgttag gtatataaaa ccaatcaaga gtagtgttat
    22741 ctaaagaaga tacatctaat ttattattat cattattagt agtggctttt gatggtttat
    22801 ctgttccgtc tgaaactatt acattggaag tatccttacc accatctatt ttttccttat
    22861 cttcttgtaa ctgtgtttcc ttattttgat tttcgttttg attgttttgt gaatttgaac
    22921 atccaacaat aaaaaaaata gaaatagaaa gtagtgttaa ataatgcaat acttttctaa
    22981 acattttgaa gctcctttct aaattaataa taattgcata tagtattatt ataattggaa
    23041 attactatta ataatagtta tatctttaaa aataaagtca caatagtatg acaaagggtg
    23101 aataatcaat aaatttaaaa aaaaatgaaa aaaatagttg accatattat gagaagatgt
    23161 tattataata aatgtgctca acaagaagta acaaaataac aagcacaaag aagttaattg
    23221 aat
//
LOCUS       DAGQWR010000062        23017 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.062, whole genome shotgun sequence.
ACCESSION   DAGQWR010000062 DAGQWR010000000
VERSION     DAGQWR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.062"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            827..1042
                     /locus_tag="MIW95_002046"
     CDS             827..1042
                     /locus_tag="MIW95_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBY3151372.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKSYIVEDLIQVLEEKGVM"
     gene            1042..1521
                     /locus_tag="MIW95_002047"
     CDS             1042..1521
                     /locus_tag="MIW95_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBY3151373.1"
                     /translation="MIVYHDSYCVAKTPNIIFLRDSLDFGKGFYVTSIKTQAINWIER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFILECRNMSDVYMKYDVIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRCEEEV"
     gene            1527..1757
                     /locus_tag="MIW95_002048"
     CDS             1527..1757
                     /locus_tag="MIW95_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151374.1"
                     /translation="MEANKTLLQILYKKIILEFSNQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(1873..3039)
                     /locus_tag="MIW95_002049"
     CDS             complement(1873..3039)
                     /locus_tag="MIW95_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBY3151375.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHMQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENRLEILTLEQQKNFIKAIEGHKLEVLFLVALGTGLRLGELLGLKW
                     FDIDFKNSNLTVKRTLQRTYFIDKNGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNVDILKAGELYRNDNYVFCNELGVPIDDKRPLRNLKSILSSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(3029..3781)
                     /locus_tag="MIW95_002050"
     CDS             complement(3029..3781)
                     /locus_tag="MIW95_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBY3151376.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYIIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNNLFISDISIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            complement(3873..4364)
                     /locus_tag="MIW95_002051"
     CDS             complement(3873..4364)
                     /locus_tag="MIW95_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3151377.1"
                     /translation="MINNTIGEKIKQYRKSINMSRNELANKIGCSVHAIAKYEQNQRI
                     PSLDMIRKISTALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVVHILENYGYDI
                     KTDYKKKFEITDNADTIKPISLSFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            complement(4615..5379)
                     /locus_tag="MIW95_002052"
     CDS             complement(4615..5379)
                     /locus_tag="MIW95_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3151378.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYFLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCVSNTELDFSLHPDDI
                     KEIIKNDVLIKQSIDELLDIGLSEENIEAIYDYFSEATDNGSNIRLKKLISQKENMTI
                     KNNDYFKLLQENNELKEKNKKLENTLKEIKKLLTKI"
     gene            5526..5726
                     /locus_tag="MIW95_002053"
     CDS             5526..5726
                     /locus_tag="MIW95_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3151379.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKEE"
     gene            5730..6044
                     /locus_tag="MIW95_002054"
     CDS             5730..6044
                     /locus_tag="MIW95_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151380.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            6066..6809
                     /locus_tag="MIW95_002055"
     CDS             6066..6809
                     /locus_tag="MIW95_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151381.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSIIREYDKEAVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYHSKEIVINKETGIYNPDDSQLKW"
     gene            6822..8156
                     /locus_tag="MIW95_002056"
     CDS             6822..8156
                     /locus_tag="MIW95_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY3151382.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVEELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFTSY
                     VSIVIEKYKNRNIIDKFNKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEMNEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDDEIVKYIQALSIINQENNLY
                     INDSVNTVSGIKREIKSTKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            8406..8678
                     /locus_tag="MIW95_002057"
     CDS             8406..8678
                     /locus_tag="MIW95_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151383.1"
                     /translation="MYDEIIEIYFDYWINEHMYRSILEKDEYYIECWKEVKKINELLE
                     INLEDDKKYLLVELNDLFNSLIEFERELSYKLGFKTGAKFIIELLK"
     gene            8951..10078
                     /locus_tag="MIW95_002058"
     CDS             8951..10078
                     /locus_tag="MIW95_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBY3151384.1"
                     /translation="MIIPKLEPNDRKREFNKYSSEQINKVVYNYLFKNNMSNRKIDDE
                     ILDLSSKQSKGYQSMGIVHHLGINKNHKGIFTGYTIDKAIEVLKEDEIHFKQIINILL
                     EIRNINEYPIIVDSWELLDEKIALKTIDKSTLKHNGTGVPIEIRYFFEINNLGKNEKK
                     NVNFKYNDNYYSAVIEMDKLENPRSRIIWKSKFRDLLRSIFSEYYEKFLENKIEGKTN
                     LPKIRFIKLDSNNFNIEFIFTAEIEDDVLEEISQDNPLNINTINIELETEIRKEGKVK
                     YVYGKQYERNPRNRIEAIKYHGTKCIVCGFDFEKTYGDRGKGYIEIHHIKPLSSVGEE
                     SNINPKTDLVPICSNCHRMIHRKKDNVLSIDDLKQIIKDSK"
     gene            10558..11205
                     /gene="cas5"
                     /locus_tag="MIW95_002059"
     CDS             10558..11205
                     /gene="cas5"
                     /locus_tag="MIW95_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBY3151385.1"
                     /translation="MILNKIEIIGDMAHFKIPMHSKIQRTYEIPPISTVVGILKNIYG
                     EEIDDFILGYVIYHDGTFKDLQTIYKEINPNVKTLTDSDRFQTDVCPIEYLINPKLII
                     YTNIDKNIEFNEPLCLGKTNCLAKVLSLSEIEVNLIDKKAIGYNQYTDINIGDGMIKR
                     INTLTEYNEKKGYYDIYSSMVRENDEFEYGKYYDEDEQQNIFLWKWKKGGVIKCI"
     gene            11196..11960
                     /gene="cas6"
                     /locus_tag="MIW95_002060"
     CDS             11196..11960
                     /gene="cas6"
                     /locus_tag="MIW95_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBY3151386.1"
                     /translation="MHLVRRHLILTVDNEVVLDYNYQYELMKRIYEAIEINDKRKALS
                     LHNEGYKVDKKVFKLFNYTIMFENAKYLKEGIHLNPQTKIKLILSGYDDILNNIIKGF
                     IKCKVFKLNNIEFKVSDIEEDSKKNFNNITLYKVRSPIVASLYDLKSRKQVYLNPMQE
                     EFYKALHDNLGNKYKLIHNKEYTGELYFDIEDVLAVKKKYITNIKGKGFIIGYTDFEI
                     FVQANKDMQEVIYYCGLGEKNSIGMGLLTYITSRRA"
     gene            11962..13128
                     /locus_tag="MIW95_002061"
     CDS             11962..13128
                     /locus_tag="MIW95_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151387.1"
                     /translation="MDRSYNVFVDNGLYVLAYYLNKDINDITYQDIENSIDLMSDKIE
                     EFVSCEKYSNLKSMCFSNSTLTQPKGKVTLNEKLKGFVKNQGDEYCSLCGQYKAKVKI
                     EDKEYNIGRSYMPNLVANTFYNFSNNLQGLNVCPYCLVLTMYSILNCRVSRYAFLYNS
                     TSSEFMEDYTFHIQKENLTDVELGAKKEKEKYSIVKLLESLVCKYNLFNGDIEQYMFN
                     NSGQSQDIQVNRIKNKYVNLLIKLQEKALLSNFEKLHLDRYILNGTLESNYLREVYRV
                     KKEEKMDEKEQKNFDELVIFLNREVNVLKKVKIDIIKKLTDKLKTSDLDIDKIYKELK
                     MLGKFYAYEDWLVNLNEMYYEKTGEKLFEAEDYIELDDIRSFRQIKNLILVSLI"
     gene            13149..14219
                     /locus_tag="MIW95_002062"
     CDS             13149..14219
                     /locus_tag="MIW95_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HBY3151388.1"
                     /translation="MENKNSNRMLNIGYAIEVLCGRLNGSSSEVEKKNNSYKNSTYTK
                     KIGSRSRISSQCQKYNQKLFMEQVCGYEQNVKTKDGKQVKLVPNPFKYATDDIFGFMM
                     AEKLSIEEDVYKELSDEEKEIYKKNKKKYEVNITKKRKSRWQMNSLINVSNRKVEWEW
                     NVCGTTGDSMPYQVEVYSGVHTNIANLNIGKIGEYDISDIASEHRDYTTLEAKSIGVE
                     KLDKEEKFKRIEALLKSLEYLSVEGNKTNHLTDTKPKFVILGEYSWGNNVFQGIIKEN
                     GLDVEMLKESIEQNEEFRKSDIWIGINKFYDDTFNEIEVNNEKEQIKKYLEEEFEEYD
                     FMKISNVHQAFESYLKYLKETL"
     gene            14230..16215
                     /locus_tag="MIW95_002063"
     CDS             14230..16215
                     /locus_tag="MIW95_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBY3151389.1"
                     /translation="MNNLYEYKAKSDGTSIDKHNEDMNYIMNQLVSIYNISNDMKNDL
                     YEAIVCHDVGKVVKSFQDNIESSTRRVRHELLSASIKYLNRGQKLAILTHHKDIKFLE
                     GQITNSIYEIELKELKELLDIEVEDIRPFIKEISRARNDDTRKLENILLKGYLQYCDH
                     IASAGIKSIDKGFNSIKDFSIPKGVSYNSIQEKVLNMDKNEDIMIMAMTGLGKTATSL
                     YWSNLVQNEYKSKRVYYILPYTASINSLYENLYNRNISVSMLHSKAPYFLNKISDEED
                     IKQLYQIYKKSVKQINVCTIFQLVKSVFSCKRFEMLLSQLKDSIFIVDEIHCFDTRVL
                     CYTLEFLKYLKENLNVKICIMSASIPTCLKKLIQERLQISTIIEPDSKDLIFRHRLNR
                     LSEGILDSIHKIENDLMQGKRVLICVNSVDLSQELYGRLQKYNPKLIHGRFNTRDREQ
                     AEKGIEESFLLIGTQSIEVSLDISYDVLYTELAPLDSLLQRFGRVNRRGDNAELSNIY
                     IYDYDRKLNIYDEDIINRTDIVISDIIHNDNSIVDESKVPSYLDNVYLDIDMDKYNKH
                     KVALEIIMKNMRLGVWNKSANNDMLNNDTISVVPECLLEEYKLYIDKKMYLEASSLFV
                     NVSVKRYNYNKKMFYECGDIVVTSYSYNDKLGLIFDC"
     repeat_region   16468..17090
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=16468..16496
                     /rpt_unit_seq="gtttaaattacactaagttagttataaat"
     gene            17535..19091
                     /locus_tag="MIW95_002064"
                     /pseudo
     CDS             17535..19091
                     /locus_tag="MIW95_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444896.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            20269..21483
                     /locus_tag="MIW95_002065"
     CDS             20269..21483
                     /locus_tag="MIW95_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HBY3151390.1"
                     /translation="MGEKMQSVICKIFDNKLLISNDLSEQDDVLYEVMDKFIDDIEYI
                     SKLGDEQIKSMLMEVNKFEEDSTEFELYMKNLCYLKQSYFIDTNDKSEINSIDIDILK
                     NVKIEDLVDITNIKLERNLNINIEHISKAVNKIYEENEKWKEYNIQNTMSKYIGIPES
                     EVIFISNLSQYVSFLSELDNAGDYVSRGQKDCMYELLPSLHRKYKKDYGIHSDEYEEA
                     FKQKIIYYDKEIKNRSSEELRAEGQHFGLPTEYLDFTEAHLISLLFAIEDYKYTKQHS
                     IVYFIDSISYNTDTIKRSEKLVNYSDTSAIDNCSKFSSRSFFIKVGNSNERIHFQKGC
                     FLKVSSEDKGEFKEKLKKYCKVVVINKDAKKEILKELFNLGITFENIYPDKDNVVKSI
                     KFYYDEIVGGNL"
     gene            21483..22775
                     /locus_tag="MIW95_002066"
     CDS             21483..22775
                     /locus_tag="MIW95_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBY3151391.1"
                     /translation="MREQFKNSIKNIKSNRPLVSVQDIVNFIDKGEKYLKLSIDEIDE
                     MEILQGIVMSPDYQRTYKSSNKEESSIIESLLIGIPIPEIFLVKIGVNDMQIRHVMDG
                     QHRLNAIYRYVKNKYPLKNLDILGNDPLYDNKRFSELEKKDKIKILGSHLSILEFDGF
                     DNPEIEIELFKRYNRNTKPLEMQEIEMATYFSETSKYISKFINTLIKDKYKDEIDKSE
                     YKYANYKSDLLRIYNITKSRNDKQKNHQEICIIFSILENGLQENIRDGVTASKKFLEY
                     KSIQYKNNESEKLELLKDKFDEFNRMILKISEKIEYPFSTSLISDDKQRQSKYLIGVS
                     IVLASICYYYDIDLENNYLIQDIKKIISCSPIADIEYKASSTNMKNMILYLFIKNKIQ
                     DNKFESLNLKRDKIKEINTLVMNNDGYLNEQTRLLEIY"
BASE COUNT         9462 a         2236 c         3527 g         7792 t
ORIGIN      
        1 ataatatcaa ttttaaaata tgtatataga taattgagtt tttttatcat cttgtacgca
       61 gtatttttat taacaacgat attttttgat aattttctat agctaatatt ttcatcaaat
      121 ataaataaat taatagcaag tagccatttt ctgtagtcta gtttagtttt atgcataatg
      181 gtattagttg taattgaaaa cttactattg caaatattgc aatgaaataa atcattataa
      241 gtactaattt tatcagaata acaatagggg catattaatg agttttttaa tttaagatta
      301 tataaaaaaa tcttataaga gacatctgtt ggaaagtttt tataaatatc aaagtctttt
      361 gtcataattt aattatatca tgttgtagaa attaatttac tgtatttaag tgggtcaaat
      421 ttaatatata tgtttttgat agtgtaaaaa ttaaaatata attttaggca tgtttgtatt
      481 gaataatgag cactttcaaa ttttattagt gtaagaggtt tcaaattata aagtaatgaa
      541 tgtatattac tatatgattt ataattagaa taaaattttt ataatagaaa tttaatatat
      601 caaaatatca gttgaggata ttgttcaagt ggtatatctt ccctgagtta aaaaataaat
      661 gatgaaatat ttggaaatgc tagtctttat ttgtttatga tattatgtaa catagaattt
      721 taatatctac tttatgatat aatattttta taaacataag atggtttaag ttattaatta
      781 ataaaaatat atatattata taagtggata ggagttgagg tttgtaatgg atgaaagaaa
      841 actagaattt tctatttttt gcattgaagc tttagcagaa aagttgaata tagattcaga
      901 aggagtttat aaacttctta aacatgatac tgatatttta gataactata taataccttg
      961 ctatgaagca ctacattctc aaagtaaaag ttatatagtg gaagatttaa tacaagtgct
     1021 tgaagaaaag ggcgtgatgt aataattgta tatcatgatt catactgtgt ggcaaaaacg
     1081 cctaatatta tcttcttaag agattcttta gattttggaa aaggattcta tgtaaccagt
     1141 ataaaaacac aggcaattaa ctggatagaa cgttttaaga aaaggggtaa aaaagcatat
     1201 ttaaatagct attctctaga tattgataaa ttaaaagaaa actgcaaggt aaaaatcttc
     1261 aatagttata atttagaatg gcttgacttt atattagaat gtagaaatat gagtgatgta
     1321 tatatgaaat atgatgttat aataggtgga attgctgatg ataagattta caatacaata
     1381 gaactgtatc aagataatct tattgaaaaa gatgaagctt taaaaagatt aaaatactat
     1441 aagccaaatg aacaaatatg tataactaat caaaatgtaa tagataaata tttaaagttt
     1501 agatgtgaag aggaggtata gtgcttatgg aagcaaataa aacattatta cagatactat
     1561 ataaaaaaat tatattagaa ttttctaatc agacaggcaa gagtttggaa gaaagtatgg
     1621 attatttata tacttctgaa acatataaac ttataagtga gggtgtatca gatatgcact
     1681 gtaagggtca tatatacttg gctcaagaat taatgctaga aaatggatta atgtatcata
     1741 aaggctatcc aagataaata gttaaaacta gatacgaatt aaggatacaa gataatataa
     1801 aattaatttg tatccttatt tgggttttgt aattaactat atgaaatcat agtattttta
     1861 tgtatttgat ttttacaatg aaaatatttt atccaacttt tctacagctt caaccttctt
     1921 atcttccata acttgagtat atatatttaa agttattgat atatcataat gacccattaa
     1981 aacttgtaca gttttaggag gaacatcatt ctcaaaaagt ctagttgcat aagtttttct
     2041 aagaccatga aactttatag gttctatatc taagctgctt aatatagatt ttaaattcct
     2101 taaaggtctt ttatcatcta tagggacacc taattcattg caaaatacat aattatcatt
     2161 tctatataat tctccagcct ttaatatatc cacattttga tttactttat gctctttcaa
     2221 tttatttaaa acatcttttg gaataggtac agtcctgtaa gaatttgatg tctttggttc
     2281 ttgctctaat actttaagct ctctatttcc atttttatct ataaaatatg ttctttgaag
     2341 ggttctttta actgttaaat tagaattttt aaaatcaata tcaaaccatt taagtcctaa
     2401 taattctcct aatctaagtc cagtaccaag agcaaccaaa aataatactt ctaatttatg
     2461 cccctctata gcttttataa aattcttttg ttgttcaaga gtcaatattt caagcctatt
     2521 ttcctttttc tcaactggta tatttaccat agtacaataa ttacgttgta taagaccttg
     2581 tttttcagct tctgataagc aggttttcag tttagtgttt atttgcctta tagtaggtat
     2641 acttttattg gaatcaagca atctattgta atatttttgc atgtgagata ctcttaagtc
     2701 ctttaactta acattgccta aatcagtatc tttaatataa ttacgataga tgctttcata
     2761 tctttcaaat gactttggtt ttaaatcttt tattctatat tcaaacaacc aattgtaaaa
     2821 ccaatgctct aaagttattt tatcatctga aatatcagaa cttaaataat attgtttttt
     2881 ataagcatca agtttatctt taacttcctt ttgagtttta ccataaaatt cttttcttat
     2941 tggttttcca tcttcattat aaccaatcat tatacgagaa cgccaaccat ctttatattt
     3001 tccgatagag ccttctccat tagctctttt agttgccatt tttaaacaac tccttatatg
     3061 atttatagtt atatccatcg aagaatgtaa tacatttctc taataagaat tttataactt
     3121 catcagcttc tagtgtttca tagaagttta gcattaatgt gttgtattct tgtttatgtt
     3181 tggctggtat tgatagaact cctatatcat tacttataag tattaggttt gaggtaagtc
     3241 gagcagttct tttgttacca tcatagaaga attgacaata ggacaaccat aaatttatta
     3301 ttatagcctt ttctaagggg ttagttattt tatttatgat acttatgtca cttataaata
     3361 aattatttaa tagagtggct tctatgcact catattgagt agtaccagct atacctatgt
     3421 taccgtttct aaaatctcca acaattagag cttcatcttt tgcaactatt ttatgtatag
     3481 agcaaattgt atctttattt aaagataagt tttctttgtt acttaatttt attatataat
     3541 cccatgatga ttttatattt agtatttgat tttggtcact taatttatgt ccacctactg
     3601 ttataccatc aagtaatgtt tgtacttctg ggaatgtgaa ggggttattt tctaggttac
     3661 ccatatcata tacaaattca cttatcattc ttctaatgta tgttaaagta tactttttat
     3721 ctccatgagg tagattcgta gggaatttat gtgtgtattt tggtatatat gatttattca
     3781 tgattttagc tcctttcata tctaattttt atttattaac tatgaatgta taaaagttaa
     3841 taggtaaacc catattttga aaatgtgcaa tactaatatt cttcatcctc atcttcaata
     3901 agaccattag agttttcttg tataaaattt tcaacaagac catttatcac ccaatttaga
     3961 tttttggaga agtctttgaa gtcagagaag cttaagctaa ttggttttat tgtatctgca
     4021 ttatctgtaa tttcaaactt ttttttatag tctgttttta tatcataacc ataattttct
     4081 aatatgtgaa ctactaaatc caactcttta tcaaatactt ttaaattaat attattattt
     4141 gttaaagtat ctaaatcatt ttcttcaaca atgagtttat ttatggcaat tgataaagca
     4201 gtagatattt ttcttatcat atctagacta ggtattcttt gattttgctc atatttagct
     4261 atagcatgaa ctgaacaacc aatcttgttg gctagctcat tccttgacat atttatcgat
     4321 ttcctatatt gtttaatctt ttctccgatt gtattattta tcataattcc accttaaata
     4381 tatcatcaaa caatgattgt atttttacac atataaagga gttattttct agattatcta
     4441 tatcatatac taacttactc ataattcttt taatgtatgt tagagtatat tttttatctc
     4501 cataaaataa attagtaggg tatttatatg tgtattttag catatatgct ttattaataa
     4561 ttgtaactcc atttgtagtt ttttatcatt gctttagata gtattgatat tattttatat
     4621 tttagttagt agttttttta tttctttaag tgtattttca agctttttat ttttttcctt
     4681 taattcatta ttttcttgta ataatttaaa gtaatcatta tttttaatag tcatattttc
     4741 tttttgagat attaactttt ttagtcgaat gttactccca ttatctgttg cctcactaaa
     4801 gtaatcatag attgcttcta tattttcttc tgatagacca atatctaata attcatcaat
     4861 tgattgtttt ataagaacat catttttaat aatttctttt atatcatcag gatgtaatga
     4921 aaaatctaat tcagtattag atacacaatc tgaaagtatc caattataaa cttgattttc
     4981 atttagttct aatttttcac caagtttata atattggtct ttatcagacc aagcacctag
     5041 caataaactt gaggtaatcc taatatcatc atcttccata agatttgaaa tagcatttaa
     5101 aaattcatca aaagaatagt attcttctgg gaataaagac tgtaaaaaat ataaaacatc
     5161 ttcattagtc attacttcat tttcaatatc aaaataaaag atagtctttt caatttttaa
     5221 agctttagcg agttttgata aggtatctat actaggtatt cttttatcat tttcataatt
     5281 tattagagca tttcttgaga tgccactaat ttcagcaagt tgaatctgtg ttagttcttt
     5341 ttgttttctt atctttttta agttttttcc aatttgcaat attatcacct cgtataaagt
     5401 atatcccaaa agtacacaaa tgagaacaga aaaataatat attcttattg acagcacaca
     5461 tatgtcgaca ttataattaa aatgtaaagt ttacgaatgt aaactagaaa aaaggaggaa
     5521 aaagtatggg aatcaattta aaaatattta ggattaaaaa aggtttaaag caacaagaac
     5581 tggcagatag agttggtata tcaagatatt atttgtcaaa cttggaaaca ggtaaggcta
     5641 ataatccaag caatgatttg atgattaaac tatctagagc tttagataca acagtagaag
     5701 aattattttt tagtaaggag gagtaaaata tgaaagagaa taataattta gaagttttaa
     5761 agacattaca agctacttta aacattattg taaagaatga agaagaagca caagaaaaaa
     5821 agcaaggata taaaattatt gaaaatgcga aaagaaataa agctgaactt ataacatgta
     5881 aagaagcatc taagctttat cctataggag aatcaaaatt taggcagtta tgtcactcta
     5941 aaaataaagg tttcccttgt atatggataa ataatcgagt atacataata aaaaacaaat
     6001 tggatgattg gtttattgaa aatgcaaatg gtatcaagtt atagtcttaa agtaggtgtt
     6061 tgcaattgag caataaaggg tggataaagc tttacaggga gttgttggat aaatcaattt
     6121 ggaaagatat taaaccagaa agaaaagtaa tattaataac tctattactt atggctagtc
     6181 ataaagaaaa tcatgttttt atagataata aaaggataaa aataaagtct ggacaattta
     6241 tttcttctta tagcaaaata gctgaaagat gtggtaaagg agttagttat caaatggtta
     6301 gaagttgttt gttggttttt aaaaatgctg gttttctaac atacgaaaca acaaacaaat
     6361 atatagtcat aactattgaa aactgggagc tttaccaaga gaacatagaa aatcaacaac
     6421 ctaatcaaca gacgattaac aatcaatcaa caacaatcaa gaatataaag aatgaaaata
     6481 atattatata tagtgaaatt ataagcaaat ttaactcaac ttgtattgaa ctttcaaagg
     6541 tcaagaaact aacagacaca agaaagagga gaataaattc aataataaga gaatatgata
     6601 aagaagctgt attagaggtt ataaatatag ttagtaaaac aagttttctg aatggaaata
     6661 ataatacagg atggaaagca aattttgatt ggataatgaa ttcaagtaat tttaccaaga
     6721 tactagaggg caattatcat agcaaggaaa ttgtcataaa taaagagaca ggaatatata
     6781 atccagatga ttcacaatta aagtggtaag gaggttatat tataaataat tcaaacaact
     6841 taattaaaaa tacagaagca gagcaatcaa tattaggaag cattttatta gactcaacaa
     6901 tagtagaaga gttagagcaa atgttaaatg aagacatgtt ttatcatgag gaacataaat
     6961 acatatttaa tgctataaaa gaaataaata agagaaatga agtaatagat ataataactc
     7021 ttacagaaga attaaagaga aaaggtcatt tgaataatat tggaggtata agctatatta
     7081 ccagtttgtc aacaatagtc ccaacagtat caaatttcac gagttatgtc agtatagtta
     7141 ttgaaaaata taaaaataga aatataattg ataaattcaa taagttcaat cttggagaaa
     7201 tagatgataa tactcttata tatcagttag aaaatgatat aaataaagcc aatcagaaat
     7261 taataaaaaa ggattcaagt ataacagcca tatcagagag agcctttgaa tatataaatg
     7321 atgaaatgaa tgaaggtatt aagactggaa taaagttctt agatgatact ataggaggtt
     7381 tatatggagg tgaactaact acaatagcag ccaaaagtgg aagggggaaa acagcattag
     7441 ctttacaaat acttagaaat gtaatatttc aaggaaagaa agtacttttt ataagtggag
     7501 aaatgagtga tatacagatt ctgtttagaa atatagcctc actaactgga atatcagttg
     7561 taaaaatgaa aaatagaaat ctaaatgatg atgaaatagt caaatatata caagctttaa
     7621 gcataataaa tcaagaaaat aacctttata taaatgactc tgtaaataca gtatcaggga
     7681 taaagagaga aataaagagt actaagccta gcttagtcat aattgattat ttgcaaattt
     7741 tggattatga aggtaaagaa gtaggagaag ctaaatttgc tgacttatca aggcaaataa
     7801 aaaagattac tttagatttt aatataccag ttattcagct tgctcaatta aatgatgaat
     7861 tgaaggatga aagacctaaa ggggatagag taatgagaag ctcaaagcag atatatatgg
     7921 attctaattc agttatatat attcatagac ctacagaaaa agaggctctt aattattgca
     7981 agacaatggg aattgaggat ataagagagt tgagacgaag agaaaaaggt tttggtgcta
     8041 atttaagtga gattattgtt gccaaaaata gagatggtga gtcaggattt agaccatatt
     8101 ggtatatagg aaatttgttt tcttttgaag aatgtaagaa gatacaagag atataaaaaa
     8161 gaagcatgat ttattaagta atagataaat tatgtttcaa gaagtattga atggtaaata
     8221 tataaaactt aattagatgt aatagagatt taaaatatat tgagtttaga tttaaatgct
     8281 tattagaaaa aggtagttgc catataaata taacaactac ctttataaat taggtactta
     8341 atgtacacta acctagcaac ttaagtatat attaagtacc tgatgaaagt caaggaggta
     8401 ctaaaatgta tgatgaaatt atagaaatat attttgatta ttggataaat gaacatatgt
     8461 atagaagtat tttagaaaaa gatgagtatt atattgaatg ttggaaagag gttaaaaaaa
     8521 ttaatgagtt attggaaata aatttggaag atgataagaa atatctgttg gttgaattaa
     8581 atgatttgtt taattcatta atagaatttg aaagagaact tagttataaa ttaggattta
     8641 aaacaggagc aaaatttata atagagttgt tgaagtaatt aatttgtgta tctcaataga
     8701 ataattaaat agtaaatcga gattattaat aagttgtact agaatatttt aagtgaagta
     8761 tatattaatt atattgttta aaataaatat atttaataaa gaggggataa gtatgccctc
     8821 ttttattttt atgcaatcaa tgacattgta ggatttacgt attttgataa atagcacaat
     8881 atattttaat aataaatata tgatacaata attttattaa aatctaaaat aatcaattga
     8941 ggaggagaat atgataatac ctaagttaga gcctaatgat agaaaaagag agtttaataa
     9001 atactcatca gagcaaataa acaaagtagt ttataattat ttatttaaaa ataatatgag
     9061 taatagaaag atagatgatg agatactaga tttatcatca aaacaaagta aaggttatca
     9121 atcaatgggt atagtgcatc atttaggaat aaataaaaat cataaaggta tttttacagg
     9181 atacacaata gataaagcaa ttgaagttct taaggaagat gagatacatt tcaagcagat
     9241 tattaatata cttttagaaa ttagaaatat aaatgagtat cctataatag ttgactcttg
     9301 ggagttatta gatgagaaaa tagctttaaa aacaattgat aaatctacat taaaacataa
     9361 tgggacagga gttccaatag aaataagata tttttttgag ataaataatt taggaaagaa
     9421 tgaaaagaaa aatgtgaatt ttaaatataa tgataattat tatagtgcag ttatagagat
     9481 ggataaatta gagaatccaa gaagtagaat aatttggaaa tctaagttta gagatttact
     9541 aagaagtata ttttctgagt attatgagaa attcttagaa aataaaatag aaggaaaaac
     9601 aaatttacct aaaataagat tcataaaact tgatagtaat aattttaaca tagaattcat
     9661 ttttactgct gaaatagaag acgatgtatt agaggagata agtcaagata atcctttgaa
     9721 cattaatact attaatatag aattagaaac tgaaattaga aaagaaggga aagttaaata
     9781 tgtttatggg aaacagtatg agcgtaaccc cagaaataga atagaagcaa taaaatatca
     9841 tggcacaaag tgtattgtat gtgggtttga cttcgaaaaa acgtatggag atagaggtaa
     9901 gggatacata gaaattcatc atattaaacc attaagttct gttggagaag aaagcaatat
     9961 aaatcccaaa acagatttgg tgccaatatg ttcaaattgt catagaatga ttcacaggaa
    10021 gaaagataat gttttgagta ttgatgattt gaagcaaatt ataaaagata gtaaataatg
    10081 cttatgtgtg gcagagatgt ataaagtata tattctaaac tagttaacaa caaatactaa
    10141 tataaaaaat caaaaaattt attaggaaaa ggtagttgct aaagtataac agctactttt
    10201 ataaattaag tatttgaggt atattagtct aaccgattag gtatatctta agttattgat
    10261 gaaaattaat taagactaac atgtatgatg aatccataga aatgtatttt tattgttaaa
    10321 taaatgaaca tacatagaaa tattttagaa aattacaagt actatattga aagtttgaag
    10381 gagaaccttg atttaaggta agtgtaaagt ttttagaaac ttcactaaaa taatctaatt
    10441 aagttatata tttgttactt ttaggaaaat aatggtaaaa attaaaggtt tttataaatg
    10501 aatgtctaat tttataaata aaatggagta ttttaactaa atatttgagg agataaaatg
    10561 atattaaata aaatcgaaat aattggagat atggctcact ttaaaattcc tatgcatagc
    10621 aagatacaaa gaacatatga aattccacca atatcaacag ttgtcggaat tttaaaaaat
    10681 atatatggtg aagaaataga tgattttata ttaggatatg ttatatatca tgatggtaca
    10741 tttaaagatt tacagactat atataaagaa atcaatccaa atgtaaaaac attgacagat
    10801 agcgatagat ttcaaactga tgtatgtccc attgaatacc taattaatcc taaacttatc
    10861 atatatacta acatagataa aaatatagaa tttaatgagc cgctatgttt aggtaagact
    10921 aattgtttag cgaaggtatt atctttaagt gaaattgaag taaatctaat agataaaaaa
    10981 gctattggat acaatcaata tacagatatt aatattggag atggaatgat aaaaagaata
    11041 aatacattga cagagtataa tgaaaaaaaa ggttattatg atatatattc ttcaatggta
    11101 agagaaaatg atgagtttga atatggtaaa tattatgatg aagatgagca acagaatatt
    11161 tttttatgga aatggaaaaa agggggagta attaaatgca tttagtaaga agacatttga
    11221 tactcacagt ggataatgaa gttgtattag attacaatta ccagtatgag ctaatgaaaa
    11281 ggatatatga agctatagaa attaatgata aaagaaaagc actaagtctt cacaatgaag
    11341 gctataaggt ggataagaaa gtatttaaat tatttaatta cacaattatg tttgaaaatg
    11401 ctaagtattt aaaagaggga atacatttaa atccccaaac aaaaatcaaa ttaattttaa
    11461 gtggatatga tgatatattg aataatatta taaaaggttt tattaaatgt aaagtattta
    11521 aattgaataa tatagagttt aaagtttcag atatagaaga agattcaaag aaaaatttta
    11581 ataatataac actttataaa gtaaggtcac ctattgtagc aagtttatat gatttaaaaa
    11641 gtaggaaaca agtatactta aatccaatgc aagaagagtt ttataaagca ttacatgata
    11701 atttaggaaa taagtataag cttatacata ataaagaata tactggtgaa ctatactttg
    11761 atattgagga tgtattagca gtaaaaaaga aatatataac taatataaaa ggaaaaggat
    11821 ttataattgg atatacagat tttgaaatat ttgtacaagc taataaagat atgcaagaag
    11881 taatttatta ttgtggatta ggtgaaaaaa atagtatagg aatggggtta ctaacttata
    11941 taacttctag gagggcttaa tatggataga agttacaatg tatttgttga taatggtcta
    12001 tatgtgttgg cttattatct gaacaaagat attaatgata taacttatca agatatagag
    12061 aatagtatag atttaatgag tgataaaatt gaagaatttg taagttgcga aaaatactct
    12121 aatttaaaaa gtatgtgctt tagcaattct acgctaactc aaccaaaagg aaaagttact
    12181 ttaaatgaga agttaaaagg ttttgttaaa aatcaaggag atgaatattg tagtttatgt
    12241 ggtcaatata aggccaaagt caaaattgaa gataaagaat acaatatagg gagaagttat
    12301 atgcctaatt tggttgcaaa tacattttat aatttctcca ataatttgca aggtttgaat
    12361 gtatgtccat attgtttagt attgactatg tattcaattc tgaattgtag agtaagtagg
    12421 tatgcttttt tatacaattc tacatcaagc gaatttatgg aagattatac tttccatatt
    12481 caaaaagaaa atttaactga tgtagagtta ggagcaaaga aagaaaaaga aaagtattca
    12541 attgtaaaat tattagaaag tctagtgtgt aaatataact tatttaatgg tgatatagag
    12601 caatatatgt ttaataactc tggtcaatct caggatattc aggtaaatag aataaaaaat
    12661 aaatatgtga atttattaat aaaattacag gaaaaagcac tactatcaaa ctttgaaaag
    12721 ttacatttgg ataggtatat cttgaatggt actttagaaa gcaattatct cagagaagta
    12781 tatagggtaa aaaaggaaga aaaaatggat gagaaagaac aaaagaattt tgatgaactt
    12841 gttatttttt taaacaggga ggtaaatgta ttgaaaaaag tcaaaataga tataataaaa
    12901 aaattaacgg ataaattaaa aactagtgac ttagatatag acaaaatata taaggagctt
    12961 aaaatgttag gaaaatttta tgcatatgag gactggttag ttaacttaaa tgaaatgtat
    13021 tatgaaaaaa ctggagaaaa actatttgaa gcagaggatt atattgagtt ggatgatata
    13081 agaagcttta gacagattaa aaatttaata ttagtaagtt taatttaata atattatgga
    13141 gggttaaaat ggaaaataaa aattcaaata gaatgttaaa tataggctat gcaatagaag
    13201 ttttatgtgg gcgattaaat ggaagtagta gtgaagtaga aaagaaaaat aattcatata
    13261 agaattctac ttatactaaa aaaataggga gcagaagtag aatctcaagt caatgtcaaa
    13321 aatacaatca aaagctattt atggaacaag tttgtggtta tgaacaaaat gttaaaacta
    13381 aagatgggaa acaagtaaaa ttggttccaa acccatttaa atatgcgaca gatgatatat
    13441 ttgggtttat gatggcggaa aaattatcta tagaagaaga tgtatacaaa gaattatcag
    13501 atgaagaaaa agaaatatat aaaaaaaata aaaagaaata tgaagtaaat ataactaaaa
    13561 aaagaaagtc aagatggcaa atgaactctt taataaatgt atcaaataga aaagttgaat
    13621 gggagtggaa tgtttgtgga actacaggag atagtatgcc atatcaagtt gaggtatatt
    13681 caggggtaca tacaaatata gcaaatctta atataggaaa gataggagaa tatgatatat
    13741 ctgatatagc aagtgagcat agagattata caactttaga agctaaatca attggagttg
    13801 aaaagttaga taaagaagaa aagtttaaaa gaatagaagc tctcctaaaa tcactagaat
    13861 acttaagtgt agaaggtaat aagacaaatc atctaactga tacaaaaccc aaatttgtta
    13921 tattgggaga gtattcatgg ggaaataatg tatttcaggg tattatcaaa gaaaatggct
    13981 tagatgtaga gatgttaaaa gaatctatag aacaaaatga agaatttaga aaaagcgata
    14041 tatggattgg aataaataaa ttttatgatg atacatttaa tgagatagaa gttaataatg
    14101 aaaaggaaca aataaagaag tatttagaag aagagtttga agagtatgat tttatgaaaa
    14161 tatcaaatgt acatcaagct tttgaaagtt acttaaaata tttaaaggag actttataaa
    14221 ggatatatca tgaataattt atatgaatat aaagctaaat cagatgggac aagtatagat
    14281 aagcataatg aagatatgaa ttacataatg aatcaattag ttagcatata taatatatct
    14341 aatgatatga aaaatgattt atatgaggca attgtatgtc atgatgtagg gaaagtagta
    14401 aaaagctttc aagataatat tgaaagtagc acaagaagag ttagacatga gttattatca
    14461 gcaagtatta agtatttaaa tagaggtcag aagttagcta tattaaccca ccataaagat
    14521 ataaagtttc tagagggtca aataacaaat tctatttatg aaattgaatt aaaggaatta
    14581 aaggaactat tagatattga agtagaagat ataagaccat ttataaagga aatatcaaga
    14641 gcaagaaatg atgatactag aaaacttgaa aatatattat taaaaggtta cttgcaatat
    14701 tgtgaccata tagcttctgc gggaattaaa agtatagata aaggatttaa ttctattaaa
    14761 gattttagta taccaaaggg tgtaagttat aattcaatac aagaaaaagt tttaaatatg
    14821 gataaaaatg aagatataat gataatggct atgacaggtc ttggaaaaac agcaacatca
    14881 ttgtattgga gtaacctagt tcaaaatgaa tataaaagta agagagtgta ttatatactt
    14941 ccttatacag caagcataaa ttctttatat gaaaatttat ataatagaaa tataagcgta
    15001 agtatgttac acagtaaagc tccatatttt ttgaataaaa ttagtgatga agaagatata
    15061 aaacaacttt accagatata caaaaagagt gtaaaacaaa ttaatgtatg tactatattt
    15121 caattagtaa agtcagtatt ttcttgtaag agatttgaaa tgctactatc acaattaaaa
    15181 gatagtatat ttatagttga tgaaatacat tgttttgata caagagtatt gtgttataca
    15241 ttggaatttt taaaatattt aaaagaaaac ttaaatgtaa aaatatgtat aatgagtgca
    15301 agtataccaa cttgcttaaa aaagttaata caggaaagat tgcaaataag tacaattatt
    15361 gaaccagata gtaaagattt aatatttaga catagattga atagattaag tgaaggaata
    15421 cttgatagca tacataaaat agaaaatgat ttaatgcaag gaaaaagagt attaatatgt
    15481 gttaattcag tagatttatc tcaagagtta tatggaaggc ttcaaaaata taatcctaaa
    15541 ttaatacatg gtcgatttaa tactagagat agagagcagg cagaaaaagg gatagaagaa
    15601 agcttcttat tgataggtac tcaaagtata gaggtaagtt tagatataag ttatgatgtt
    15661 ctttatacag agttagctcc tttagattca ttgttacaaa ggtttggtag agtaaataga
    15721 aggggagata atgctgagtt gagcaatatt tatatatatg attatgatag aaagcttaat
    15781 atatatgatg aagatataat aaataggact gatatagtaa tatcagatat aatacataat
    15841 gataattcta tagttgatga gagtaaagtt ccatcttatt tagataatgt ctatttagat
    15901 atagatatgg ataaatataa taaacataaa gttgctttag agattataat gaaaaatatg
    15961 aggttagggg tatggaataa atctgctaat aatgatatgt taaataatga tactattagt
    16021 gttgtgcctg aatgtctttt agaagagtac aagttgtata ttgataaaaa aatgtattta
    16081 gaagcgagtt cattatttgt aaatgtatct gtaaaaagat acaattataa taaaaagatg
    16141 ttttatgaat gtggagatat agttgttaca agttatagtt ataatgataa actaggtcta
    16201 atatttgatt gttaagagaa ttatagtata actaattaaa atattttatt gagaatttgt
    16261 tataatttat atcgactaca aattttatat gttacaatag aataattttg cagtgagcga
    16321 aaaatatgtg taaatggctg tgagttagtg atggcaaggt ttataactga atttttgttt
    16381 ttagctaaaa acactactgg tgcctcactg caaattaact attttaataa ccctacaagt
    16441 gttgtaatta ctatagtttt atttggggtt taaattacac taagttagtt ataaatattc
    16501 cggaaagtac ttttgctatt tgattcattc tacccgttta aattacacta agttagttat
    16561 aaatttcact aattttagtt acaactatta caagtccagc cgtttaaatt acactaagtt
    16621 agttataaat tatgtgtaat gtattttaat gttttctagc aacaatgttt aaattacact
    16681 aaattagtta taaattttat aaattctatg aataatttta aagagaggtt tcgtttaaat
    16741 tacactaagt tagttataaa ttgtacattt gacatagaag cttcaaaatc catcccagtt
    16801 taaattacac taagttagtt ataaatgtaa cagcacgaat gatggcacag acctatctgc
    16861 aatgtttaaa ttacactaag ttagttataa atagaagtac acttacaagt aagataaaag
    16921 acccaggtac agtttaaatt acactaagtt agttataaat tctgtagcta tatgtggaag
    16981 taattttagt agagaggttt aaattacact aagttagtta taaattattg aaggggtgag
    17041 agttgtgact aggcactaca agtttagatt acactaaatt agttgtaaat ttacttattc
    17101 attaagaaac ttaactgaga gtttgattta tattattgaa ttagcaagag ttttagaaag
    17161 tagaaaatta taccccaata ataccccaac cacaaaaata aataataaaa aatatccaaa
    17221 aaaattagta taacatcata actaaatagc tatataccta cacttacgaa aaccaacaaa
    17281 acaatcaaat aaccacttgt agactcccct cgtctccacc aattttgaaa atgaaaataa
    17341 tagaataaaa ggaattactt atgtttggac taagtaattc cttttatttt gcttaaaagc
    17401 acacaattag cacacaacta gaaggttata taaataaaaa ggctgtcgca ctagaaaata
    17461 gtgctacgct cttttttgta taaaaaaata aatcccaagt tcaaaagaac ctgggattct
    17521 tgtataataa ctgtatgcta aaacaaaata ctacacaaat tgattatact acttatggtg
    17581 ataattatca atttaagcta ccactaaata ttgattacat gattccaaaa gatgattctg
    17641 taaggttatt aagttaaatt attgaggaga tgaatttaga aaaattatat aagacttatt
    17701 ctcgaattag atgaaataga gtaatccata gacaattttt gaaagttata atatatacag
    17761 atatgaactg tatttattct tcgagagaaa tagaaagaac atgcaaaaga gatattaatt
    17821 ttatgtacct tctaggcgaa acctccactc cagaccattc aacaattgca aaatttcgca
    17881 gtatacattt ttaactaata ggcaaaagct tgattgcaca gtttacaaac tttcttggag
    17941 ataataatga aatttcaaaa gacacaatat ttattgatgg tacaaaaata gagtcatctg
    18001 ctaatagata tacttttgtt tggaaaaaag ctgtaaataa aaatatggct aaaatggtga
    18061 ataatatatc tgacttcata ttgaaatgtg agattgatgt tggcattaaa atagtttatg
    18121 aagataaaat taaaatctat catttaaaga aactaaaaaa aatatcaaaa gaagcaaatg
    18181 tagcatttat tcatggaaaa ggaaatagaa aatctactaa aaaccttcat ataatgggtg
    18241 aacgaaatag ttgttctaaa acagataacg atgaaacttt tatgagaatg aaagaagatc
    18301 atatgaaaaa tgggcaactg aaaccagcat ataatataca atttggtgta gattaagaat
    18361 atatagtttg gctaaattca ggaaatcaaa ctgctgatac tacaacatta ataccatttt
    18421 taaaatctat agaggaatat actaagttta aatatcctaa agtaatagct gatggtggat
    18481 atgaaagtga ataaaactat gcatttttag agcataataa tcagttatct ttcataaaac
    18541 ctgcaaatta tgaaattatt aaaactagaa aatataaaac ttatattgga tgtattgaca
    18601 atatggatta tgaagaagaa aataattatt atatttgtac aaatgagaaa aaactatttg
    18661 ctaataaaat aataaatagg aaatcaaaaa caggatataa aagtgaaata acatgttaca
    18721 tttgtgaaga gtgtagtaat tgtcaatata aaagcaattg tattaagaga agtaatagta
    18781 aggttcctct taaaaatagg actaaaaact tacaagtatc taagtcgttt catgaaaaaa
    18841 gaaaagaaaa cttaaagaga attatgagcc tcgaggggga tgaacttaga gtgaatagaa
    18901 gtatacaagc agaaggagct ttcgctcaag taaaataaaa tatgaaactt acaagatttt
    18961 taagccaagg taaagaaaat gttttataag gatatattat tgtggcgata gcttataacg
    19021 tttctagact acacaataaa atagtaaaaa ataaacgtgg aatatatctt tatcatttaa
    19081 aaaaatattc taaaattatt atttataatc aaatattatc tgaaaaaatg gtatttttat
    19141 atctttattt gtcatgcata attttactta gaacagaaac tttatataaa acaattgaaa
    19201 atatacctat taagataatt agaaaagagc tctcacagaa caaatttaat ttgctcatgc
    19261 aacagcccca tattgatata catttaggtt gtataattat acatttcaat attacatgtt
    19321 cattaggata atagttagta aatgatatga ttgagagaat agatataata aactaatcta
    19381 gtcctgtatt gcaatgttta ttaaagaata tatttttaaa gatccaagta ctgcatctct
    19441 tccagaattg agttggttaa tagttttttg atgctatgca atttataata aaaagtataa
    19501 tgatttagtt gatgaatcat cagaatttat accctcaaag attaatcaga ttgcaataaa
    19561 aataatgata tattctatag ttataatatc tattttcttt gcaacccctc atacaatgaa
    19621 tataaatata tcaaacttgg atatgggtat aattctcatt tcagttttat ttatacagtt
    19681 tttatctaaa ttaattttgt atgtatattt tgataaaagg gagatatgta actaagtggt
    19741 aatattggtg aaaacaaaaa ttaaagaata tagaaaaaaa ctccttatga cacaaaatga
    19801 acttgctaaa tcagttaggg taaggtgtga gactattgta cacttggaaa atataatcct
    19861 tctttaaagt tagtgataga tattgctaaa gtattcaata ctacagtaga aaatttgtct
    19921 gaatttattg aagaaggata gtggatagat tataaatagt tataagatgg ttaatgaaat
    19981 atagttactt aattattaag tgtgttagaa atatttatat gtaacacaat gtgtagttaa
    20041 gattacctta tctcatctaa gactttaatt tttcttccca taaagagggt atatcaattt
    20101 tctagtgtgg tagcccattt tttattgatt taaatttata atatatatta atagatgata
    20161 cttaaaaatt ttataaaata tctaagttgt cataaataaa acgatatatc taaccgaata
    20221 tggtaaaatt tgttataata tagataagtt agagtaaaaa taattttaat gggggagaaa
    20281 atgcagtcag taatatgtaa aatttttgat aataaattac taattagtaa tgatttaagt
    20341 gagcaggatg atgtattata tgaggtaatg gataaattta tagatgatat tgaatatatt
    20401 tcaaaattag gagatgagca aattaaaagt atgcttatgg aggtaaataa atttgaagaa
    20461 gattcaactg aatttgaact atatatgaaa aacttatgct atttaaagca atcatatttt
    20521 atagatacta atgataaaag tgaaataaat tctatagata tagatatatt aaaaaatgtt
    20581 aaaatagagg acttagttga tataactaat attaaattag aacgaaattt aaatataaat
    20641 atagaacata tatctaaagc tgtaaataaa atatatgaag aaaatgaaaa gtggaaagag
    20701 tataatatac aaaatactat gtctaaatat ataggtatac ctgaatcaga agttatattt
    20761 atatcaaatt tgagtcagta tgtttcattt ttatctgaac tagataacgc aggcgactat
    20821 gtatctagag ggcaaaaaga ttgtatgtat gaattattac catcattaca tagaaaatat
    20881 aaaaaagact atggtataca ttcagatgaa tacgaggaag cattcaaaca aaaaataatt
    20941 tattatgata aagaaataaa aaatagatcg agtgaagaat taagagctga agggcaacat
    21001 tttggactac caacagaata tttagatttc acagaagctc atttaatatc attattattt
    21061 gcaatagaag actataaata tacaaaacag cattcaattg tgtactttat agactctatt
    21121 tcttataata ctgacactat aaaaagatct gagaagttag taaattattc agatacatct
    21181 gcaatagata attgtagtaa attttcatca agatcatttt ttataaaggt agggaattca
    21241 aatgaaagaa tacattttca aaaaggatgt ttcctaaaag tatcatcaga agataaaggc
    21301 gaatttaagg aaaaattaaa aaagtattgt aaagtagtag ttattaataa agatgctaaa
    21361 aaagaaatat taaaagaatt atttaattta ggtattacat ttgaaaatat ttatccagat
    21421 aaagataatg tagttaaatc tataaaattc tattatgatg agatagtagg gggaaattta
    21481 taatgagaga acaatttaaa aattctataa aaaatattaa atcaaacaga cctcttgtat
    21541 cagtccaaga tattgtgaat tttattgata aaggagaaaa gtacttgaaa ttatcaatag
    21601 atgaaataga tgaaatggaa attttacaag gaatagttat gagtcctgat tatcaacgta
    21661 catataaatc atcaaacaag gaagaaagtt ctataattga atctctttta attggaatac
    21721 caatacctga aatatttctt gtaaaaatag gtgtaaatga tatgcaaata agacatgtaa
    21781 tggatgggca gcatcgtttg aatgctatat ataggtatgt taagaataaa tatccactta
    21841 aaaatttaga tatattaggc aatgacccac tatatgataa taaaaggttt tcagaattag
    21901 agaaaaaaga taaaattaaa atattaggaa gtcatttatc aattctagaa tttgatggtt
    21961 ttgataatcc agaaatagaa atagaattat ttaaaagata taacagaaat acaaaaccat
    22021 tagagatgca agaaatcgag atggcaacat atttttcaga aactagtaag tatataagta
    22081 aatttataaa tactctaata aaagataaat ataaggatga aatagataaa tcagaatata
    22141 aatatgctaa ttacaaatca gatttactta gaatatataa cataactaaa tctcgtaatg
    22201 ataaacaaaa aaatcatcaa gaaatatgta taatatttag tattttagaa aatgggctac
    22261 aagaaaatat cagagatgga gttacagcct ctaagaaatt tttagagtat aagtcaattc
    22321 agtataaaaa taatgaaagt gaaaaattag agttattaaa agataagttt gatgagttta
    22381 ataggatgat actgaaaata tctgagaaga tagaatatcc attctcaaca agtttaatat
    22441 ctgatgataa gcaaagacaa agtaaatact tgataggagt atctattgtg ttggcttcta
    22501 tttgttatta ttatgatata gacttggaaa ataattattt aatacaagat attaaaaaaa
    22561 taatatcttg ttcacccata gcagatatag aatataaggc gtcttcaact aatatgaaaa
    22621 atatgattct atacttattt ataaagaata aaatacaaga taataaattt gaatcattaa
    22681 atttaaaaag agataaaatt aaagaaataa atactttagt aatgaataat gatggttatt
    22741 taaatgaaca gacaaggcta ttagaaatat attaacaagt tataaaataa atcatggtaa
    22801 tgtaaaataa tttgtgcttt taaggttaat aaaaatcttc tttatggcaa ttatttaaat
    22861 ataatcaaat gtcgtaaagg agatttttta tgaacgcttt aagaaaacaa ttagccaaag
    22921 aaattatatc agatatctaa aaaagagcat gtcaaataaa accctaggta tttaaatttt
    22981 tttagagcta cttaaaataa attatgctat ctctgta
//
LOCUS       DAGQWR010000063        22735 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.063, whole genome shotgun sequence.
ACCESSION   DAGQWR010000063 DAGQWR010000000
VERSION     DAGQWR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.063"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            385..3933
                     /locus_tag="MIW95_002067"
     CDS             385..3933
                     /locus_tag="MIW95_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY3151392.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINNEMVIALMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTISCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKIIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDNFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADMALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESASNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMRNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEVTSIEAEENT"
     gene            4118..6052
                     /locus_tag="MIW95_002068"
     CDS             4118..6052
                     /locus_tag="MIW95_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBY3151393.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6158..7153
                     /locus_tag="MIW95_002069"
     CDS             6158..7153
                     /locus_tag="MIW95_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY3151394.1"
                     /translation="MKKYISIILLVVVTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSNIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7125..7910
                     /locus_tag="MIW95_002070"
     CDS             7125..7910
                     /locus_tag="MIW95_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBY3151395.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7898..8596
                     /locus_tag="MIW95_002071"
     CDS             7898..8596
                     /locus_tag="MIW95_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151396.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8719..9192
                     /gene="ybaK"
                     /locus_tag="MIW95_002072"
     CDS             8719..9192
                     /gene="ybaK"
                     /locus_tag="MIW95_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBY3151397.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9389..10228
                     /locus_tag="MIW95_002073"
     CDS             9389..10228
                     /locus_tag="MIW95_002073"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBY3151398.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10241..10789
                     /locus_tag="MIW95_002074"
     CDS             10241..10789
                     /locus_tag="MIW95_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBY3151399.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10855..12036
                     /locus_tag="MIW95_002075"
     CDS             10855..12036
                     /locus_tag="MIW95_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBY3151400.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYIYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12209..12472
                     /locus_tag="MIW95_002076"
     CDS             12209..12472
                     /locus_tag="MIW95_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY3151401.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            12998..13681
                     /locus_tag="MIW95_002077"
     CDS             12998..13681
                     /locus_tag="MIW95_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3151402.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGEFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            13692..14933
                     /locus_tag="MIW95_002078"
     CDS             13692..14933
                     /locus_tag="MIW95_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3151403.1"
                     /translation="MKIFSNKDIKIFFLILISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIVLNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISINGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSTFTISFLKLTNM"
     gene            15005..15688
                     /locus_tag="MIW95_002079"
     CDS             15005..15688
                     /locus_tag="MIW95_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151404.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            15685..18015
                     /locus_tag="MIW95_002080"
     CDS             15685..18015
                     /locus_tag="MIW95_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY3151405.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDSGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANTGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNRNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAMSGCLLGSILGLMLHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPVKKICELDIIDSINAQ"
     gene            complement(18196..19038)
                     /locus_tag="MIW95_002081"
     CDS             complement(18196..19038)
                     /locus_tag="MIW95_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBY3151406.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKAGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            19333..20067
                     /locus_tag="MIW95_002082"
     CDS             19333..20067
                     /locus_tag="MIW95_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY3151407.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            20077..21210
                     /gene="nagA"
                     /locus_tag="MIW95_002083"
     CDS             20077..21210
                     /gene="nagA"
                     /locus_tag="MIW95_002083"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBY3151408.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNITHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            21309..22058
                     /gene="nagB"
                     /locus_tag="MIW95_002084"
     CDS             21309..22058
                     /gene="nagB"
                     /locus_tag="MIW95_002084"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBY3151409.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKLENTHLP
                     NGMADDIEKECLNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            22233..22505
                     /locus_tag="MIW95_002085"
     CDS             22233..22505
                     /locus_tag="MIW95_002085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151410.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPIGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
BASE COUNT         9079 a         2377 c         3978 g         7301 t
ORIGIN      
        1 attttatgta tacaaaatta atttgctaac tgaatcatat aaaaaaaaca aaatattttt
       61 tagagggaat gattagatac tattttcttt gttatgaaat taattgaaga gtaattaatt
      121 agaaaaggaa taaataataa tgaatatatt acattattac tttttgataa atcaaaaata
      181 aagttatagt ttttaatatt aattaagaat gatgaaatat gaataaagat tttacaaata
      241 aatcttaagc tatcttattt aattctaatg caggaagaag gtaaatgtat attgaaaaaa
      301 tcatagaaat agaagtaaac aacttaaaca aatacaaagg tttgcaaaag aagtaatata
      361 attatataca atgaggatgt gagtatgatg aacagactaa agccaagaat tttaggtatt
      421 agtatcttgt ttattattat aattatttta acatgtggag ctgttttcaa gtttaaattt
      481 aaattagata ataaattaaa agaaaccaca catacatatt tgtctgagtt tacagaacac
      541 aatataatta atctcaagac aagacttagt ggtcaatttg acatgcttga atctattgct
      601 aacttcatag gaaacttgaa taatataaat aatgaaatgg tgattgcttt aatggattct
      661 gatgtaaagc gaagttcttt aattagaatg actatctcta cattagatgg ggaatgttat
      721 tctaatgatg gaatcaccca taatgttcaa gatagagatt attttaaaaa agctaaaaat
      781 ggagaaaaga atatttcaga gcctttaaaa tctattgtta ataataaaga aataatagtt
      841 ttatctaccc cagtttataa aagtgggaaa atagttggtg ttttaagtgg aatttatgat
      901 tcaaaaaagt taaatgagtt attaggatta tctgcatttg atgaaaaagc agatgttttt
      961 gttgcaaaga aaaatggaga tatagttgct caaaaaacaa ataaaaatag aaagacgata
     1021 tcatgtatat ttcagctttt tgacaaagat aattgtgatg aagatgattg tctaaaaact
     1081 ataaaagaaa atatgaaaaa tgataagtct ggatatttta attataactt agataatgaa
     1141 aaatttatgt taaactatca gccacttgga gttaatgatt ggtatgtttt ttctgttgtg
     1201 ccaaataaaa tagtgtctat tcagtcagag aatatatctt atgatgtata tatttttgca
     1261 ttagaaatac tactagttat attgattttt ataatatata tagcatgtat tataaataat
     1321 aacaccaaaa ttattataaa tgaaaaagaa aaatttaaag tactcactga taatatatta
     1381 ggaggggtta aaacctgttt aaaagatgaa tatatgacta tgacatatgt aagtcagggg
     1441 tttttagagc ttacaggtta ctcttgtgaa gatttaaata aaatatttaa aaatagattt
     1501 aaagacatga tttataaaga tgatttagac aagtttaatt gtattattct aaatcaatta
     1561 gaaaaacaag ataaaattga agttgaatat agaattgtaa gaaaagatgg aagtataatt
     1621 tggattttgg agagaagtaa gtctataaat gataataaag gaagtgagtg tattcaggga
     1681 gttttaactg atataacaga acttaagaaa atacaagaag agttagattc aaaaagattt
     1741 gaaatacagg ctatcaatga tagtattgtt ggtggaatag tgataactga gataaatgat
     1801 gattttgata gtatatattt aaatgaaggt tatttaaata tgattggata tacacgcaaa
     1861 caactggaag atgagctaaa taataaatta atcaaactta tttttaaaga tgatagaaca
     1921 gaattaatta atagtataaa caaacaactc aaattaagtg atacatttgc atctgaacaa
     1981 agagttgaaa aaagggatgg tactattata tggattttat taaaaggaag aaagataatt
     2041 tatgataatg gaaaatctgt actttgttgt gttattgtag atatcacttc tgctaaatta
     2101 ttagagcaag aacttaaaaa gagtgaagaa cgtcatgaaa tcattctaaa gcagacgcaa
     2161 aatatcataa tagaatggga tatagaaaaa gataaaatga atatatcacc taattggtat
     2221 aataagttta aatatgaccc tataactgaa aacgcaacaa aaaatataat taaatcagta
     2281 gatatatatc atgatgatat ggataagttt atttcattaa ttaaacttac ttcacaaggt
     2341 agtgagtatg aagagtgtga aattagactt agaactagag atactattta cgcttggttt
     2401 agtataaagg caacaacttt atttgataat aatggtaaac cttataaagt agtaggaagt
     2461 ttgactgata ttgatgaatc aaaacgagaa gctgaaaagt tgaaagaaag ggcagaaaaa
     2521 gaccctctta cagatttgta taataaaaaa gcatcacaat cacttataga agagtacata
     2581 acattggata aaaaaagaaa aggtgcttta atgattattg atattgataa ctttaaagga
     2641 ataaatgata atttaggaca tttatatggg gatgctgtat taagtgaaat tgcaaatgac
     2701 ctaataaatc tatttagagc aaccgatata gtaggcagaa ttggtggaga tgagtttatt
     2761 gtatttataa aagatatttc tgaaacaaga gatattataa ataaagcaga agaattggta
     2821 aaaacatttg aaaggtcttt tttaggtaaa ggacgaaatt ataaaatatc attaagtgta
     2881 ggaattgcta agtttccaca agatggtatg gactatatga cactatttag aaatgcggat
     2941 atggctcttt actcagccaa agggaagggt aagaattgtt atacattgta tgataaaaaa
     3001 attgataaag tccaatatat aactaataca agaaatgcag gagatagtga taatgtagag
     3061 atgtctttta aagaacatat cacaaattat atatttgata tactatatga atcaagtgat
     3121 atagaagtgg cagtaaattt aattctaagt attgtaggta aacactttaa tgtgagtcgc
     3181 atgtatatat ttgaaaattc agatgataat cgagtatgta aaaatacatt tgaatggtgt
     3241 aacgaaggtg taacctcgga aattaataat cttaaaaata tttgttacaa ggatttaggc
     3301 aactatcttt taaattttaa tgcagatggg ttattttatt gtaatgatat tgaaaattta
     3361 ggtagtgatg tgaaaaaagt acttaagcca caaggcatac gttcaatatt acaatgtaaa
     3421 attttagagg gcaatgaatt tagagggttc ataggatttg atgaatgttc atataatcgt
     3481 atgtggacta atgatgaagt gaacactttg acatttatat ctaaaacgct cagtatgttt
     3541 ttattaaaaa tgcgtattca gagtaagcta gaagagtctt taacaattca aaaatctgtg
     3601 atggataata tggatatatg ggcatatgtt gttgacagga atacctatga attattattt
     3661 atcaataaaa aaatgcttga gtcagcatca aacactcatg taggtgattt atgttataaa
     3721 gcgatatgga ataatcaaga taaaccttgt aaaaaatgcc ctatgatata tttaaaagat
     3781 ggtgatgata cttataaatc atttatgcgc aataaaaagt ttgaggtaca ggcaaatata
     3841 actacaacta ctttgaaatg gtttgatggg aaagatgcat gtttaatatt tggaagtgaa
     3901 gtaactagta ttgaagctga agaaaatact taaaatactg tgttatatat atacttaaaa
     3961 tcgcaatttg aatttattag acttaaaatg tctatataaa tcatttaagt tgtggtttta
     4021 agtatttttt atgatataat taagcgttaa gaacaaaatt aaaagatact tttgtataaa
     4081 aaaatatttg ttaaatatat attgagcagg tgataatatg gaaaataagt ttttaccaat
     4141 aagcaaacaa gatatgatag atagaggatg ggaagaatta gattttgttc tagttacagg
     4201 agatgcttat gtggaccatc atagttttgg aacagcaata atatctagag ttttagaaaa
     4261 tgctgggtat aaagttggaa taatagctca accagactgg agaactacag atgattttat
     4321 gaagctagga aaaccgagac ttgcatttct agttaatgca ggaaatatgg attcaatggt
     4381 caatcattat tctgtaagta aaaagcatag agaaaaggat atgtattctc caggtgggaa
     4441 aatgggttat agaccagata gagctactat agtatattgc aataaaataa gagaagcata
     4501 cagtgatgtt gcaatagtaa taggtggaat tgaagcgagt ttaagaagat ttgcacacta
     4561 tgattattgg agtgataagg ttagaaagtc tatgttaata gatagtggag cagatttatt
     4621 agtttatggt atgagtgaaa agcagattgt agaagtagcc aatgcactta atgatggata
     4681 tgacccaaaa tatattagac atattgatgg gacatgctat atttcagata cattggaaga
     4741 aatatatgat aaatatatat taattccatc atataaagaa atttgtgaag ataaaatgaa
     4801 gtatgtagaa gcttttaaaa ttcaatatga tgagcaagac ccatttagag gtaatataat
     4861 tgttcaacct catggaagta agtatctggt tcaaaataag ccagaaaaac cacttagtag
     4921 agaagaacta gatgaagtat atggattacc atatcagaag acataccatc ctgtgtatga
     4981 aaaatttgga ggaataccag ctatagaaga agtaaaattt agtatagtaa gttcaagagg
     5041 ctgttttggt agttgttcat tttgtgcaat aacatttcat caaggtagag cagtgcaaag
     5101 tagaagtgaa aaatcaatta ttgatgaagc aataggaata acaaatttag atgattttaa
     5161 agggtatata catgatgttg gaggtccaac agctaatttt agaagaccag catgtaaaaa
     5221 acaaattact aaaggtgctt gtaaaaatag acaatgctta tctccatcac catgtaaaaa
     5281 tttagatgca gaccacagtg aatacttaca tttacttaga gctgtaagaa aattacctaa
     5341 aataaaaaag gtgtttgtgc gttctggaat tagatacgat tatgtaatgg cagataagaa
     5401 caataaattt cttagagagt tgatagagca tcatgtaagt ggtcagttaa aggtagcccc
     5461 agaacatata tcagaagaag ttttaaagta tatgcaaaaa ccagcaggaa aaacttatga
     5521 taaatttaga cagaagtttt ttgctataaa tgaagaatta ggtaaaaagc aatatttgat
     5581 accctattta atgtctagtc accctggttc taccttaaat agcgctatag agttagcaga
     5641 gtatttaaga gatactcact accaaccaga gcaggttcag gatttttatc caactccagg
     5701 gactctatct acaaccatgt tttatacagg tattgacccg cttacaatga agccagttta
     5761 tgttcctaaa tcaaaacgtg acaaggcaat gcaaagggct ttattacaat atagagcacc
     5821 tagaaattat gatttggtat actctgcgtt agttgaagca ggaagagaag acttaattgg
     5881 ttttggacat agatgtctta taaagccaaa aaatgaaaag ccttatttta atagaaataa
     5941 ttctaaaaaa aatgtatcta aaggaacaaa taaaaacaaa aaaactaata caaataacag
     6001 aagtaataaa aatcaacaaa aaagttcaac taaaaaaaag aaaaaacatt aaagtataaa
     6061 taaaaatact taaaaaagaa ggattattcg ataaattaac agaaattagt ataaataaat
     6121 ataattattt taatgacgtt acctttggga ggaagagatg aaaaagtata tttctataat
     6181 attattagtg gtggtgacta tggttgtttt ggtaggatgt tcaccaggaa aagataaccc
     6241 taaaaataag gagctatcag ttgtcaaaca agtaaaagta gctgttccag atggactacc
     6301 agcagtggca atagctaaac ttgcaaatga aaatccagag attaaagaag gatatgaaac
     6361 agtgtatagc attgaaaaga cacctgaggc gatttccact agagtgatga aaaaagatgc
     6421 agatatagct atagttcctt caaacatggc tgcaatagcg tataataaaa cttcatcata
     6481 taatattgtt ggaactgtag gaatgggttc actttatttg gtgtcaacag aaaatataaa
     6541 agattattct gatttagttg gtaaagaagt tggatgtact ggaaaaggac ttactccaga
     6601 cataactata aaaagtcttt tgttacaaaa ggatataaat tattcaaata taaaatttaa
     6661 ttatgtaaat tcagcaagtg aactggtacc attattggct actggtaaag ttaagacagg
     6721 tattgttcca gaacctgcac ttagtgcttt gatgtctaaa aatccagaca taaaaattat
     6781 aaaaagttta aatgactcat ggaaggaagt aagtggttca aaagacggtt atcctcaatc
     6841 tactttaatt gtaaagtctg aatttttaag agataataaa gattttgttg attcttttgt
     6901 aggtcaatta tcaaatagta tagattgggc aaacaaaaat cctgaagaat taggtgcata
     6961 ttctgaaaaa ataaaaatat ctactgaatc aaagataata ggaaaatctc ttgaaagagc
     7021 taatttaaaa tatattccag ttaaggacat gattaaagat tataaaaatt actatgagaa
     7081 attagctaat tttgatgata agacattagg aggcaaagta ccagatgaag caatatactt
     7141 tgttgagaaa taaaaaaaat aaagaacaat ttatagataa aatggaggta acactttctt
     7201 gtgttatcct cttattttta tggcaaatta ttgctttaaa aattgataat gatatttttt
     7261 taccaacagt aggtcaagta tttaatagta taaaagagat tatgctaaat tctagtttct
     7321 atatagatat actttactct atggggagat gtttttttag ttttttactg gctatgattt
     7381 ttgccattat actttcaata gtatcttatt taaatagatt ttttagaaac ttattaaagc
     7441 caatcaatgc tttgacacgt tcaataccaa ctatgatatt ggtagtactt gcacttattt
     7501 ggtttgaaaa ggatagtacc ccttttatag ttggatttac tatagttttt cctatattgt
     7561 atgataacgt tttgggcgct atattgaata tagacaagaa cttattagaa atggctaatg
     7621 tgtataaaat aaggtttata gataaggtgt taaaaattta tttaccagcc ataaaatttc
     7681 aaattatatc tatactagta tcaacattct ctttaagttt gaaagttgct atagcaggag
     7741 aggtatatag tcaacctaca tatggtattg gaactatgat acagactgaa aaaattaact
     7801 ttaatacatc tggaatattt gcatggataa ttattgttct tttaatatct gctattttac
     7861 aaatagccca aaagttttta gcaaggagag cttttttatg gaaaagataa agttgagtat
     7921 tgaaaatata aataaaagat atgatagtcg tattatattt agagatttta atattgattt
     7981 ttatgtaaat gaggtaaatt gtatccttgg aaaatctggt tgtggaaaaa ctacactctt
     8041 gaacataata agtgggatta ttaaaaatga caccaacaat ctaaatatca aagaaaattt
     8101 aaatagagta ggaaataagc ttgaagctag ttacatattt caagatgata gacttataga
     8161 ctggttaaca gtagaagaaa atataaagat agttgtaaat aaatactata ataaaactca
     8221 attaaataaa atttgtgatg aatacttgga attggttgga attagtgatt ataaaaaatt
     8281 ctatcctcag atgcttagtg gaggtattag acaaagagta aatattgcta gagcttttat
     8341 atatccatca aaaaacatta taatggatga accttttaaa tctattgatg caaagaatac
     8401 acagcttata atggataatt tcagaaatat attaagaaaa gaaaagagaa ctgtattatt
     8461 tgtaactcat aatattgagg aagcattgtt tttagcagat aggatattta ttttaggaga
     8521 ctctccaatt agaataaaaa aaatattaaa aaattctaag gaattggaaa aaaatgaagt
     8581 tttgaagttg atatagagtt ataataatac tgtcgtttta aaatatattt ggttaaaatc
     8641 tataagacta tattaaaata gtacagaata ttcaaaactg tgtataatgt tcgtatttta
     8701 taaaaaggaa gtgaatgtat ggttaagact aatgctatga gaatattaga ttcaaataag
     8761 attgattata aagtaatgtc ttatgaagtg aaaagtgaac atgtggatgg tgttgaggtg
     8821 gcacatgata taggaagaga tgtaaatgaa gtgtataaga cattagtaac tcaaggagtt
     8881 agtaagaata tatatgtata tgtaatacca gtacatgaaa acttagactt aaaaaaggct
     8941 gcaaaagtag ctaaagagaa aagtgtagag atgattcatg taaaagatat aaataagctt
     9001 acaggttata ttagaggcgg atgttctcct ataggtatga aaaagcttta taaaactttt
     9061 gtaaatgaaa gtgcaaagaa tttagataca attattgtga gtgcaggaaa aataggatat
     9121 caaatagaac tatctccatt tgatttacaa agacttataa aagtagaatt tgtagatgta
     9181 ataaaaaaat aagaattaca tataaaatat agagcttata ttcttaataa taggtgttgt
     9241 aatattaata aaatgaataa taattttaaa ataaatattc agttacttat gtgtaattgg
     9301 tataaaaata ttgagaaatt ttaaaaacaa tggtaaagtg taagtataat cacgaatatt
     9361 ttttataaag taatggaggg atgttcgctt gagaaaaata aaatctgaac aaatcgtcga
     9421 gcaggtaaaa aaactttgta tagaagcgag cctatattta ggagaagatg ttttaagttg
     9481 tataaaagaa aaagcgaaaa gcgaaaagag tgaagttgga aaaaatatat tgaatatatt
     9541 agttgaaaat gctgaaatag ctaaagaaaa aaatatacca atatgccaag atactggtat
     9601 ggctgtattt tttgttgaaa taggacaaga agttttaatt gaaggagaca cacttactga
     9661 tgctataaat gagggagtaa gacaagggta tgaagaaggc tacttaagaa aatctgttgt
     9721 aagtccaatt aacagagtaa atacaaaaga taatacacct gctgttattc attatgatat
     9781 ggtaaaaggt gataaaatta aaatagaatt tgcagctaag ggatttggaa gcgaaaatat
     9841 gagtaagatg aaaatgttaa agccatcaga tggattggaa ggaattaaaa aattcataat
     9901 agatacagtt tcagaagctg gacctaaccc atgccctcct atggtaattg gagttggtat
     9961 aggtgggact gtggacaaat gtgctcaaat agctaaaaag gcacttttta gagaactagg
    10021 tgaatttaat aaggacgaaa atatagcaaa acttgaaagt gaactattaa cagctattaa
    10081 taaacttggt ataggacctc aaggcttagg tggtactaca acagctttgg gattaaacat
    10141 agagactttt cctacacaca tagcaggtct acctgttgtt gtaaatataa actgccatgc
    10201 atcaagacat aagaaggtag taatttagga ggaaaataaa atgataaaaa taactacacc
    10261 tgtaaatgaa attgatatag caaaattaaa ttgtggagat acaattagtt tgagtggtat
    10321 actttataca gcaagagatg ctgctcataa aagattgata gattgtataa ataaaggtga
    10381 agaattacca tttgacgtat atggtcaagg aatatattat gtaggaccta ctcctacaaa
    10441 gccaggagaa gttataggtg ctgcaggtcc aacgacgagt tatagaatgg atgatttgac
    10501 tatacctctt ttagaaagag gtcttagatt gatgataggc aagggaaaaa gaagtgatga
    10561 agttatagaa ggtatgcaaa aatacggagc agtttatctt gctgcaatag gaggagctgg
    10621 agcgtatatt tcaaattcaa taaaatcatg tgaaattatt gcatatgaag atttaggagc
    10681 tgaagctata agaaagattg tagtagaaga ccttaaactt acagtagcaa tagatagcta
    10741 tggaaataat atatatgagc aaggtcgagc aatttatgaa tgtaaataat gtttagaaat
    10801 aaatatgaat atcaaaatta aaaatagttg tgtatatttt aaggaggaaa attgatgtct
    10861 aaggattatg caaaattggc attagaaatg catgaaatca acaaagggaa agtaagtgta
    10921 gaaagcaagg tagaagttaa aactaaggat gatttatcaa cagcatatac accaggggtt
    10981 gctgaacctt gtttaaaaat acatgaaaat caagatgatg tttatatata cacttctaaa
    11041 gggaatctag tagcagtagt tagtgatgga agtgctgtac ttggattagg taacattggt
    11101 gctgaagcat ctatacctgt tatggaaggt aaagctatat tatttaaaca atttgcagat
    11161 gtagacgctt ttccaatatg tttaaagact aatgatgtag atgagatagt taaatctgta
    11221 gagttgatgg agcctgtatt tggaggtata aatttggaag acatatcttc tccaagatgt
    11281 tttgagatag aagagagatt aaaaaaatct ttaagtatac ctgtttttca tgatgaccaa
    11341 catgggacag caataatagt tgcagcagcc ataataaact caataaagtt aattgaaaat
    11401 aaaaaaatag aagatttaga aatagttata aacggagcag gagctgctgg tatagccata
    11461 gcaaagatac tacttaatat gaatgttaaa aatataatct tatgtgatag aagtggagca
    11521 ttggaagcta gtatagaaaa tctaaactat gttcaaaaag aaatgttaaa agtaacaaat
    11581 ataagaaatg aaaaaggacc tttaaaagat attataaaag gaaaagatgt atttattggt
    11641 gtttcaggtc caggagctgt tacaaaggaa atggttgaaa gtatgtctga gaagccaata
    11701 atattggcaa tggcaaatcc tactccagag ataatgccag aagaagcaaa attaggtgga
    11761 gcatttataa tgggaacagg tcgttcagac ttccctaatc aagtcaataa tgtactggct
    11821 tttcctggaa tatttagagg agcattagat gttaaggcta aagagataaa tgaagaaatg
    11881 aagatagctg ctgcatatgc aattgctaat acaataagtg atgaagaaat atgcccagag
    11941 tatatattac cagatgtatt taataagaat gtagtaaaaa atgttgctaa ggcagttaaa
    12001 gaagcagcga taaaaactaa agtaaataga atataaattt ttataactta ttaaaaacta
    12061 gaaaagtata aaagactagt gaaattatgt acagtgggat gattttatta gtctttttat
    12121 tttaaatatg acacaataat gtcatattaa gcttgatata attttaatat ggttaggagc
    12181 caaagaaaaa tataaggagt gatttaaaat ggaagaaact aaattttgcc aatgctgtgc
    12241 aatgccaatg ggacaaactg atgaattata tgggacaaat aaagatggaa gtaaaagtac
    12301 tgattattgt agttattgtt ttaaagatgg tgagtttaca gctgatataa gtatggaaga
    12361 gatgatagaa gtttgtatcc ctcatatgct tcaaagtaat aaggatatga ctgaagatga
    12421 agcaagaaca atgatgaaca atttctttcc aacattaaaa cgctggaaat aattttatgc
    12481 aaaattagtt atatgcaaaa catgaaatat ggtgttgaga ataaggggtg gtgtgcttta
    12541 tagcatacca cctgttttgt tacttgctag ttcagttatt cttgtatgct taaatatgtt
    12601 ctcatctctc tttctatttt aaattgtaaa aatctaaaat atgttattta tattctactt
    12661 ctttatccat gtctttttct acctcaagtc aaatacaact acaaaatcat cattttcaat
    12721 tcactgttta atagaattaa cagataaaga gttataaaat tttctatttt ttatataaaa
    12781 taatacaggt caagctgact tctagctact tgggatagtg tatatattta tgatagttat
    12841 aggagtaata ccaatcatta gactgttgat ttgtgtagtc ttttgtaaaa acatttgttt
    12901 taatatattg agtttttgaa aaagtaaaat tataattata ataaattaag tgtaataatg
    12961 tattagaagt aaaaataatt aaaatagagg tgattaaatg agcagtatat tgttagttga
    13021 agatgattta agcttgatat atggattaga gttctctata caaaaaagtg gatttagtat
    13081 agacatagca aggacagtaa aagaagctat gcaaatgtat gaggaaaaaa attatgactt
    13141 attgctttta gatgtatccc tacctgatgg taatggattt cagatttgcg aaaaagtacg
    13201 agaggtttct aatataccca ttatattttt gactgcttca gatgaagagg taaatgtagt
    13261 gatgggtctt gatatgggtg gcgacgatta tataacaaaa ccttttaaat taaatgaatt
    13321 aatttcaaga ataaaagcat tacttagacg ttataatgtt gcaagtaatg tgaatgaatt
    13381 aagttcaaat aatattacta ttaaattact tgaaaatcgt gtttttaaag gtgaatttga
    13441 ggtggaactt actacagcag aatataaatt attgtgctta tttatgaaaa ataaaaatat
    13501 tgtacttact agaaaaaaca tattggacaa gttatgggat ggaaatggta gctttataga
    13561 tgacaataca ttatcagtat atgtaagacg attgagaaat aaaattgaag ataatcctga
    13621 aaatcctaag tttctattga ccgttagaag aatgggttat aagtggaatg taattaagtg
    13681 agggtattaa tatgaagata ttttctaata aagatataaa aatatttttt ttaattctta
    13741 tatctatttt agtaatgttt attatagtag ggcaaatagt ggttataagt ataactaaca
    13801 attataaaaa aacgttgata gaacatgatt ataatatagc tggatactta tatagtatag
    13861 gtgttgataa atctaaaata ccatcagcat ttgttgcaca tgaagataaa tatattttaa
    13921 aaggaaaaga aatactagat acagctggat atggatttga tatagatgtt agctttacac
    13981 caagtgtaaa attgtttcaa agtaaatata taaaaactat gtctatctat ttattagtat
    14041 tttttactat gattattttc atactatata tgtatttttt taggcaacaa agtaagatag
    14101 aagaggctag ttataaaatt gatttattta tgaatggaaa tataaatgaa agactaaata
    14161 catatgaaga aggtagctta tcaaaattat ttagttcaat agatgttatg tcgacatctt
    14221 taaatacaca tatattaaaa gaaaaacaga gcagagaatt tcttaaaaat acaatttcgg
    14281 atatatcgca tcaattaaaa actccactta cagctttaat aatgtacaat cagatactac
    14341 aagaagaaag tcataatagt gatgtcgttg aagcttttgt acaaaaaagt caaagtgaac
    14401 tagaacgcat tgaaacattg attaagaatt tactaaagat tacaaagata gattcaaaaa
    14461 caatagttct caataaaaat aatgttaatc ttaaaaattt aattgtcaaa attttgtata
    14521 gctttgaaac tagggcagaa aaagaaggaa aggttatttc tataaatggt tcagaaaata
    14581 cattgttgtt ctgcgattat gaatggatgt cagaggcttt aagtaatctt ataaagaatg
    14641 cattagataa tacaaaagag aatgataaaa taacgataga gtggtttgag acgactataa
    14701 ccaccattgt ttcagttaaa gacactggaa atggaatcca tgttgaggat attcatcata
    14761 tatttaaaag gttttataga agtaagtttt caaaagataa tcaaggttta ggactgggat
    14821 taccactagt taaatctatt gtggaacaac ataatggtac tatttctgta gaaagtaaac
    14881 tatcacaagg aagtacattc accataagtt ttttaaagct tactaatatg taagataaaa
    14941 tacatcttgc agtaaggtat atagattatt attatattaa tcaattagaa aggtgagaga
    15001 gaaaatggat attcttgtgg ttaataattt atgtaaaaca tatggaaatg gatatacaaa
    15061 agtagaggca ttgaaagatg tatcctttac agtttcaaaa ggagagtttg tgactgtagt
    15121 tggagaatct ggttcaggaa agagtacact attaaatctc ttaggaggtc ttgatactcc
    15181 aacatcaggg agaatattta ttgatggtaa agaaatatgt tcaatgaaag aggaaaaaca
    15241 gactattttt agacgtcgta acatagggtt tatatttcag gcatataacc ttataccaga
    15301 actaactgtg gagcaaaata taatattccc attgttatta gattataaaa aaccagatac
    15361 tatttactta gatgaagtat tgactatttt aggtcttaaa gataggaaaa atcatcttcc
    15421 aaatcaatta tctggtggtc aacaacagag agttgcaatt ggaagggctt tgattacaaa
    15481 gccaagtatt atactggcag atgagccaac gggaaatctt gacagtaaaa atagtaatga
    15541 tgtaatttca cttttaaaag tgactgcaga aaagtataat caaaccatta ttatgataac
    15601 tcataatacg aatatagctt ctacagcaga tagaatacta caggtttctg atggtgtgct
    15661 tacagacctg ggggtgaata gagaatgaga agctacttag aattagtacc acaatatgca
    15721 aaagtacata agaaaaaaaa tagaactaca attttatgta ttattattgc agtatgctta
    15781 gttactgcta tctttggact tgcagatatg gctatacaat gtcaaaagat acaatttatt
    15841 aaagatgatg gaaattggca catagtaatt agaaatattg atgaaaagtt tatatcagaa
    15901 ataaatagta gagtagatat agctcaccat ggtagggggc aagtcgttga cagtggagag
    15961 tttaaatcaa aggagatttt gatacaaggt ttagatgaag aagtagcaaa acaatttaac
    16021 ttagaaataa taaggggtcg ttatcctatt ggggagaatg agactttaat agatgaggat
    16081 gcagttaaaa gctttaatct aaaaatagga gattttatca aaataaaatt agcagatggt
    16141 agaaataaaa attataagat agtaggtgaa tattcatcaa tatcaagctt aagagctaca
    16201 gataaacatg gattaatact ttcaatattg ggtacacaag ctattaaaaa atcagaaacg
    16261 tacgattatt attatattac atttaaagaa aaggttaata ttagaaaagc cacttctgat
    16321 attaaaaata ccttcaattt aagtgataaa caaatcggta aaaatgaaag attactttct
    16381 attataggtc aaggcgactc tgacttttct aaaccactgt atttaacagc aggattactt
    16441 tttgtacttg ttttagtagc aagtattatc atgatatata atagttttaa catgagtgta
    16501 attgagagag ttaaattttt tggtctgtta cgttgtctag ggtcatcaaa acatcaagtt
    16561 aaaaaatttg ttgttttaga aagtttaatt ttaagtataa aagctattcc aataggctta
    16621 gtattaggaa gtcttgtaac tattatatct tctatatttc ttaagtatgt taatgagtta
    16681 ttcagctcaa tgccaatttt aaaagttagt tttattggta ttgcatttgg aataattgta
    16741 gggtttttaa ctgtaatatt atcagctatt attccagcaa aaaaagcttc ccgtgtatct
    16801 ccacttagcg ctgtacgtgg gaatctaatg tccaaagaaa ttttaaataa cgggaagaca
    16861 agtaaagttg caaatacagg aagttttaaa attgagactt ctatgggatt aaatcatgct
    16921 ttttcaagaa agaaatcttt tgtacttatg acttcttcat ttgccattag tattatacta
    16981 tttttaggtt ttagtgtgct tatagacctt atgtatcaag gacttaagcc tatgaagcca
    17041 gaagctcctc atatatctat tgttgaccct aattttaagt ctgttttaac taaggaagat
    17101 attaatgaaa taagcaatat caaaggagta aagaaagtat acagtagaat gatgacggat
    17161 atagaagcta catataatgg gaagaagaca aaatcacaat taatttctta tgaaaagtat
    17221 caatttaatt gggcaaaaaa atatctatta aaaggagaag ttaatgaaaa taaactacag
    17281 tcagaaaacc ttgcattagt agaagagggg catggatttg atataggaga taagatatat
    17341 attaaaaata gaaatgctga aaaagagtta gaggtaggag gtatactttc ctcaattcca
    17401 tttgaggcta aagataaata tattggaaat attataacat ctgaaaatac ttttacgaat
    17461 ataacaggta taaagggtta tataataatg gatattcagg ttgataaaga tactcctgga
    17521 gatttaattg aaaatataag acgtgtagta gatgataact tagaggtcag agataaaaga
    17581 caaagtaact tagaggttaa aaattcgttt tatactatgg caacatttgt atatggattt
    17641 gtgtttatta ttgcgattat aagcatgttt aatatcataa atagcatgaa tataagtgtt
    17701 acaagtagaa agaattacta tggtgttatg cgtgcagtag gcatatccaa cagacaatta
    17761 agaagaatgg taatagtaga ggcaggttca tatgcaatga gtggttgttt attaggtagt
    17821 atactaggat taatgttaca taggtatatg tttatatcgt tagtaagttc aatgtttcat
    17881 ttagagtggc aagtaccttt taatttacta cttatcactg tactaactat gataataata
    17941 actttattat cagtaagaaa accagtcaaa aagatttgtg aattagatat aatagactca
    18001 ataaatgcac aataaatata gctgttgatt taaattaaaa aaacgaagta aaaatgaaat
    18061 agaaaatatt tttcattttt acttcgtttt ttatattata aaatttttgt tatgttacaa
    18121 aatttctata aagatgatgt gaatctaaac attatatgac aacgtgagca tttaagttaa
    18181 aatattttaa atatactatt ttagataagt tccttcttca tcttgataaa ttaatccttt
    18241 tttcataagc actccaagag ctcgtttaaa atttttctta cttgaattaa ataatataga
    18301 aatatcttta gggtcagatt tatcattaaa tctcatatat ccatcagaac cctctaaata
    18361 acttaaaatc ttattttcta aagtatcaag ttcatccttt ctatttcctc taggagaaag
    18421 accaagtttt ccatcttcat atatttttat gacatgtaaa ttttcaagaa catcaccagc
    18481 ttttagttca gtaaagtact cattatgaag tattacacca gcatatttat tgtctacaca
    18541 tatcattgca gaattattag tttgaaatcc atatacaact ccagttacaa tatctcctac
    18601 attgtaagta tggtctgtta ataaatatga atctatatct gtagtagcag caattctttt
    18661 gcttttgtct aaatatatat aaaataaata tttttcgtct ctaaatagtg gataagtctt
    18721 agctttaaaa ggaactagta tgtctttagg aagtcccata tctataaaag taccaatgtc
    18781 cgtattatca actactttta aatgtgctac atccccaacc ttagctaaag gcggtattaa
    18841 agttgcagca gttctgtcat cagaatcttt aaatatgaaa gcatttactt catcacctat
    18901 gtttatttca ttttttccga ttaatctatt atgaagaagt atatcgtctt ttgtgttatt
    18961 agtttgtcca tccaaaaaat atccaaattc agtttttctt tttactgtaa gtttattaaa
    19021 atcacctatg tttatcaaat taaaatcatc ctctcatttt atatttataa tttagccata
    19081 atatacttat gtagtagttt gtcacagtat ctatattagt attagtgaaa tttacaataa
    19141 ataatatagt tatttatgta tttttcaatt ctctagcttc ttatatagta taaactccaa
    19201 tttctttttt gtctttaggt tttactaaaa tttttacaaa ataagttaaa aagtattgaa
    19261 ataaaatttc ttttaaagta taattggtat ataccaaaat tggtatatac catatcgaaa
    19321 agtggtgact atatgaaaat agtaaataaa tctagtaata taccattgca tactcaatta
    19381 agttcaataa taagagagat gatagaaact ggtgagctta aagagggtga tgcaataatg
    19441 ccagaaagag aactttgcaa catacaaaat gtaagtcgta tgacagtaaa taaaaccatt
    19501 gttggattag tcacagaagg acttctttat agggtacaag gtaaagggac attcgttgca
    19561 aagcaaaaaa agaagtatca gttttcaaat gtaaaaggtt ttactgatgt tatgaaagaa
    19621 aagggagtaa acataaaaac ggatatatta tcctttgaga tggaactacc agatgattta
    19681 gttaaaagaa agcttggaat aagtgataat actacaaata tatataagat tgtcagactt
    19741 agatatactg atggagagcc atttggatta gaaatagtat atctatcaga agaaatgtgt
    19801 aaaggtctaa caaaagggat attggataat tcttcattat atagggtttt aaatgaaaaa
    19861 tatggctata agatacaaaa agctgaacaa gtaatggagc cagtaatact ttcagatgaa
    19921 gaaagtaagc ttcttgaaac agatgaaggt gctttagctc ttaaacttca tagaaattcc
    19981 tataatagag aaggtagccc tatagaatat accatatcaa tatttagaac agataaatat
    20041 caatatgaaa tagtattgag tgagtaggtg aataatatga aatgtttaat taatggaaag
    20101 ataatattaa aaaatcaaat cttggaaaat aaagtattag tatttgatga aaaaataatt
    20161 gatatagctg atagtgtacc aaaagattgt gaagttatag atgctgatgg aaaatatata
    20221 tctccagggc ttattgatat tcatatacat ggaaatatgg gaaaagatac aatggattcg
    20281 actgatgaat ctattgaaac aatttcaaaa tcaattatga gacatggagt aacatctttt
    20341 cttccaacta caatgactat ggataaagaa catgtttatg atgcacttga agttattaaa
    20401 aaagcccaaa atagaaagct tgaaggggca caagtattag gagcacattt agaaggacct
    20461 tttataaatg agaattataa gggagcacaa aatgaaaaat ttataataaa ttcaaaatac
    20521 gaatttataa aagaatataa agatgttata aaagttataa cttatgctcc agaaaaagat
    20581 attgattttg attttactag agaaataaaa agatgtacag atatagtttt atctattggt
    20641 catagtaatg caaattatga ccaagctaaa gaagctataa atttaggagt aactaatata
    20701 actcacatgt tcaatgcaat gactggttta aaccatagag acccaggcgt tgttggtgca
    20761 gcacttacaa ctaatgtgta cagtgagcta atagcagata caatccatat aaataaagat
    20821 ttatttcaat ttatactaaa taataaaggg aaagaaagac tgattttaat aacagattct
    20881 atagaagctg gtggtcttga agatggcaat tattctcttg gaggtcagaa agtcatagtt
    20941 aagggtaatg aagctagact tgaaaatgga gcattagctg gaagtgtgtt gtctttaaat
    21001 aaaatggtat ttaacttctt agataataca aaccttaaag tgaatgaagc aattaattta
    21061 gcttctttaa atccagcaac atctttggga ataaatgaca aaaaaggaag cttagaaata
    21121 ggtaaggatg cagatatagc tgtatttgat gagaatttag attgtaaaat gacactttgc
    21181 ttgggggacg tagtatataa aaatatatag tatacaaaac aaactttaaa gacagtaaaa
    21241 acaatataaa aaataataaa ttaaacttac attagttaaa gtagtagtta aaatttagga
    21301 ggaataagat gagagtatta gtgtgtaaag attatgatgg aatgagtaaa aaagccgctg
    21361 aaatgatagc agctcaaata gttttaaaac caaattcaat actgggacta gctacaggaa
    21421 gtacaccagt aggtatgtat agagatttag taaaaaaata caatgataat atagtagatt
    21481 tttcagatgt gatgagcttt aatttagatg aatattataa gttaccaata tctaatgacc
    21541 aaagctatga ttattttatg aaagagaatc tatttaatca tgtaaacata aagctagaaa
    21601 atactcatct accaaatgga atggctgatg atattgaaaa agagtgtctg aactatgaag
    21661 cttctataga tgcagctggt ggtatagatg tacaagtact tggaataggt cgtaatgctc
    21721 atataggttt taatgaacct gatactaaat ttgcaaaaag aactcatgta gttgaactta
    21781 cagagtctac aatagaagct aatgcaagat tctttaaatc aagagaagat gttcctaaaa
    21841 aagctgtaag tatgggaata ggttcaatat tgaagtctaa gaaaatattg cttttagctt
    21901 caggagaaga aaaagctgat gcagtatata atactgttta cggagatata actccagaag
    21961 ttcctggttc tatacttcaa cttcataaag acactatagt tatagtagat gaagcagcag
    22021 caagtaaact gaatccaaaa gattataaat tagtataaga atagaatgta gtttaagtga
    22081 aaaatagcta tcattgaata atcaaattta tttaatgata gctattttta tttaaatttt
    22141 ttacttcttt ttatttaaat tagttattat tgtttaaatt acttgcttat tactttttac
    22201 gaaaactcgt tatcaagaag tatccgaata agatgttaaa aattattatg tttagtggga
    22261 ttctatcaag cataataaaa ttacctataa ttgggatatt accaattgga aacataaaga
    22321 agaatacagg catcagtgat agtaaaaaag atggaaaaca aagaagaagt aatcttggaa
    22381 tatcaatata ccaactacca tcctttttgt attctctaat caataattca gttcctagta
    22441 acaaaccaat accaccagag gaaattaaat taataaaaat gtatctttta aagtcttgca
    22501 tataaccagt gtgaaattga gtatagaaat cataatttaa tttaacggct aaaaaatata
    22561 taagcaatag aatgattaaa aataaaagat atctaagagc ttttttcatt ttaaattctc
    22621 ccttcagaaa tactttattc agttttattt tgttggaatc tgattttatc atttagttta
    22681 agaaatccat aatattagtg aagaatgaaa taagtattat atatgaataa atatt
//
LOCUS       DAGQWR010000064        22515 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.064, whole genome shotgun sequence.
ACCESSION   DAGQWR010000064 DAGQWR010000000
VERSION     DAGQWR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22515
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.064"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            274..612
                     /locus_tag="MIW95_002086"
     CDS             274..612
                     /locus_tag="MIW95_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3151411.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            634..951
                     /locus_tag="MIW95_002087"
     CDS             634..951
                     /locus_tag="MIW95_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3151412.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1101..1778
                     /locus_tag="MIW95_002088"
     CDS             1101..1778
                     /locus_tag="MIW95_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151413.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2106..2873)
                     /locus_tag="MIW95_002089"
     CDS             complement(2106..2873)
                     /locus_tag="MIW95_002089"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBY3151414.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKVAVMPQNERDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            3679..4311
                     /locus_tag="MIW95_002090"
     CDS             3679..4311
                     /locus_tag="MIW95_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBY3151415.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(4682..5551)
                     /locus_tag="MIW95_002091"
     CDS             complement(4682..5551)
                     /locus_tag="MIW95_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY3151416.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(5648..6505)
                     /locus_tag="MIW95_002092"
     CDS             complement(5648..6505)
                     /locus_tag="MIW95_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY3151417.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(6720..8738)
                     /locus_tag="MIW95_002093"
     CDS             complement(6720..8738)
                     /locus_tag="MIW95_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBY3151418.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLIMERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(9009..9785)
                     /locus_tag="MIW95_002094"
     CDS             complement(9009..9785)
                     /locus_tag="MIW95_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBY3151419.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(9842..10813)
                     /gene="asrC"
                     /locus_tag="MIW95_002095"
     CDS             complement(9842..10813)
                     /gene="asrC"
                     /locus_tag="MIW95_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBY3151420.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(10826..11617)
                     /gene="asrB"
                     /locus_tag="MIW95_002096"
     CDS             complement(10826..11617)
                     /gene="asrB"
                     /locus_tag="MIW95_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBY3151421.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(11618..12625)
                     /gene="asrA"
                     /locus_tag="MIW95_002097"
     CDS             complement(11618..12625)
                     /gene="asrA"
                     /locus_tag="MIW95_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBY3151422.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEIIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            complement(13015..13704)
                     /locus_tag="MIW95_002098"
     CDS             complement(13015..13704)
                     /locus_tag="MIW95_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBY3151423.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKAKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITFDSSKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(13767..14615)
                     /locus_tag="MIW95_002099"
     CDS             complement(13767..14615)
                     /locus_tag="MIW95_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY3151424.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            complement(14634..15521)
                     /locus_tag="MIW95_002100"
     CDS             complement(14634..15521)
                     /locus_tag="MIW95_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBY3151425.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEVWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNINGSSFQELASTSSLIKKVAKIKNLNEKDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            complement(15579..16382)
                     /locus_tag="MIW95_002101"
     CDS             complement(15579..16382)
                     /locus_tag="MIW95_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151426.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            complement(16542..16994)
                     /locus_tag="MIW95_002102"
     CDS             complement(16542..16994)
                     /locus_tag="MIW95_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBY3151427.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            complement(17124..18626)
                     /locus_tag="MIW95_002103"
     CDS             complement(17124..18626)
                     /locus_tag="MIW95_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBY3151428.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSVVLAGLTGIPVNVLIVV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFIGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            complement(18659..19525)
                     /locus_tag="MIW95_002104"
     CDS             complement(18659..19525)
                     /locus_tag="MIW95_002104"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBY3151429.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            complement(19610..20281)
                     /locus_tag="MIW95_002105"
     CDS             complement(19610..20281)
                     /locus_tag="MIW95_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBY3151430.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            complement(20554..21045)
                     /locus_tag="MIW95_002106"
     CDS             complement(20554..21045)
                     /locus_tag="MIW95_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY3151431.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            complement(21067..22431)
                     /locus_tag="MIW95_002107"
     CDS             complement(21067..22431)
                     /locus_tag="MIW95_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY3151432.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
BASE COUNT         7693 a         3744 c         2201 g         8877 t
ORIGIN      
        1 atctaatatg taattaaaat aaaaaatgct ataattaaca tattattaat tcgttttatt
       61 attgtagtat ggctatgaaa ttatgaaaat atttctctaa ttaaaaatat gatttatcca
      121 tatttgttta ttgtagatac agactataca aaaataaata gcacttattt cagtataaaa
      181 acatatatag tctatattta gactaaaaaa gtataaatag tctatataaa acaacttaaa
      241 ctatgaatat aatctaaagt ggagggataa taattggcaa atataggaaa aataatagga
      301 gatagaataa aagtctatag aacaaaattg ggatatagtc aagagtttct agcagaaaag
      361 gcaggtttac atccaacata tattggtcag attgaaagag ctgagacaaa tacatctatc
      421 aatattataa tgaaaattgc tactgcttta gacataccac ttgaattgtt atttgcaaat
      481 attattacaa cagaaggcgt tgataactct gtacctttag aatgttatga aatgattaat
      541 aaattaactg aaaaagaaca aattgcaatg ctagagttaa taaagtgcat cattaaatac
      601 aaagaaatat aagtgaggga aatagtaaca ataatgaatt atataggtaa aagacttaaa
      661 gaagaacgaa aaaaagcaaa tttaacttca aaagaatttg caaatatggt tggagtatcg
      721 ccttggtata taactcaaat cgaaagtggt aaaaagaatc caagtcttaa aacattcata
      781 aaatttgtaa atatattaaa tatttctgca gatgttctta taaaagatat tacttccact
      841 ggaaaaacat atttagaaaa tgatataaat gaagagttaa aagacttaaa ttcaagagaa
      901 ctaaatctga tatcacagat tatagacatc ataaaaaaga ataaaatata attctttata
      961 ttttatataa aaaaaaaggt tatttgaaat aaataacctt tttttgtctt tttgtgttaa
     1021 cattgtctta tagtgctaat gagtactatt agaccatgtt ctatggcctt attttaatat
     1081 aaaacgattg gagataggct atgattgtga ttgaaggtag tgataaattt aagattgcaa
     1141 aagaatatat tgatgtagaa tatactcttt ttagcaaagt aacttttagg tatgaaaagt
     1201 tgaaatttaa agataatgct gaattggaaa aaattaagat gtttaaatat aaaaatggct
     1261 acatccctaa taaattaaac ctttcttttg gatatggatt ctcttcttat aaaaagcaaa
     1321 taattagaga aactgtagat actttaagat tgacagaaat tttttcaagc gagaacatag
     1381 aagatattaa atttataaaa gatggtacaa aaaaattaga aattagcata gagaaagttg
     1441 tgaaatttaa acgtcgaaaa aaaagaaatt atgtttgttg ctattgccct gatatgtata
     1501 gagatataaa actcgacaaa gaatctatca ataagatata caacagaaaa ataaaaatag
     1561 aaagagaagt taatattttt gaagatgagg atgttataat aaacaagaaa gtattgaagt
     1621 ttccaaagtc ttggacaaag aatatgcaaa aatattggtt aagtgaaaat aagtatccca
     1681 tacattctac tgtaattgat gatgatagat ataaatgttg taatgtacaa tatacaaaaa
     1741 atagagtgat aatattatat tacatatata accattaata tacataatta caaaaaaata
     1801 atgacaaata tacataatac aaatatatct tctactttag ataaaacagt tattatgaaa
     1861 acttactatc tataaataat agaaatgcta gtataccatc tataatctaa tgtctagtag
     1921 gttttaatgg catattttaa atatgccata gttgatttat aagattttct atatcacttt
     1981 caaaatacac actaaatagt tgcttttcag atataattgc ccaatttaaa ttttactatc
     2041 tgaaaagttt tttatattta atttatatct tcaaaaatac cgtctaatat ttctatataa
     2101 ttaatttaat taatactctt atgtaataga ttaagtactg aatttaactc tttaaatgat
     2161 atttgccctg gtgctgaaac actttttgca gctccaaaag tcaatgcaga accaaatatt
     2221 tcaccacaca atctacttat aacacccatt cctgacattg acattgttat tattggtcta
     2281 tctgcataaa ttttaaacat ttcatttgta gcttcaagta gcactaacac atctctttca
     2341 ttttgcggca taacagctac tttgggtaaa tctgcgccca attcctgcat tctacataaa
     2401 cgagatacaa tctcttcttt ttttggagtt ttattaaaat catgattaga tataattact
     2461 ttcacttctt ttttatgagc aaaattaact acttcatcta tgacctcatc tcccataaat
     2521 aactctacat ctattaaatc tactaaacct gtattagaaa tttctttatt caaagtagta
     2581 taataatctc ttgatattaa tttttctccc ccttctacta cacttctaaa tgtgaataat
     2641 aaagggatat catgtatata acttcttaat tcatagagaa cttccttaac ttctttaata
     2701 ttttctacat tctcaaaaaa atcaactcgc cactctataa tatccaaaca tgcatctttt
     2761 aactctttag cctcttttat aatatctttt ttatttttcc caataattgg tacacaaatt
     2821 ttaggtctac cttctccaat agtaatattt tttacttgaa cttttctttt catattctcc
     2881 tccataatca gaaattctat aggactttaa attctatttt ctgcataatt taattataca
     2941 ttaaaaatac tatacattat aaaaatacta atttaaaaat aatttttatt ctaattctta
     3001 aaacaatccc acatatttta gaaaaggtac tctataatat acaaacactt cttttttacc
     3061 taaatttatt ttaacattct ataattttca caatattctt taaattttac tcatataaac
     3121 aatattattc tggatgctaa tagaattaat ctataaatca tagtataatt atgcagaaat
     3181 aactcgaatt tcactactta taaagagatt gaatattaat gaatctaaat catttgcatt
     3241 acttcagagt tttgactaaa gttgaacacc atacaaaagt agctactcaa ttgtttatac
     3301 cccaaccaag cttaagtaat gttatataga ttcttgaaaa agaatttggt atctttttat
     3361 ttgaaaaaca aggtagaaat gtttccctca ctaaatacgg aagttttttt ctaacctatg
     3421 ttgataaagc attaggttaa ttagaaaaag tttacatata aaatatatta aattgttgca
     3481 agttttattg ttttgatata caatcaacta aacatataat ctttataaaa tactatacag
     3541 ttaaaagtct gttttaaaaa aaaatgtata aattataaat caaaatatag atactatcaa
     3601 ggagtgattc tgtgaagaga tgtattgtag tagtggaata gtcaaaaaca taatatatat
     3661 taaaggagag atttaaatat gactattcca aattcaaata aggtatatcc aagaagtaat
     3721 gattatcaaa ctgtatatct taaaaatgta ataacaaggg aaaatataga agttggagat
     3781 tacacaatat ataatgactt ttataatgac ccaagagatt ttgagaaaaa caatgtatta
     3841 tatcattacc ctataaacaa tgataaatta ataattggta agttttgctc aatagcatgt
     3901 aatgcaaaat ttctaatgac tagtggaaat cactcaatga aatcaaaatc cacatatact
     3961 tttcccatct tttatgaaga atggaaactc gatatcaacc atataacgga cgcttgggac
     4021 aataagggta acattataat tggaaatgat gtgtggatag gttatgattc tataattatg
     4081 tcaggtgtaa atattggtga tggtgcaatt attggaacaa gagcagtagt tactaatgac
     4141 gtctcaccgt attctattgt tggaggagta cctgcaaaaa ttataaaaaa gagatttaat
     4201 aatgatacaa tttcaaaatt attagaaatt aaatggtggg attggccata taaaaaaatt
     4261 caatcaaaca ttaagcatat tcaatcagga aatattgata aattagttta atattttata
     4321 atttatttat attaaacttt actttaataa tccttttata agagcttttt caaaatttgt
     4381 atattatttt aatatatata aatttaatac aacattaaat aaaataaaaa gtaaagttac
     4441 ttaataaaaa gttactttac tttttattat ttaaattcct gtttttatta taataggtaa
     4501 aattctatta aactagttca cattttcatt attattgata tattatatat gaatatacca
     4561 ttttgttttt taataaaaaa atcatgctac atcagtagta agcaactatt aatgtagcat
     4621 gattttttgt atctaaataa tcttagtatt tacctatcaa tatagattga tatatcaaaa
     4681 actatagtct tctatattct tctcttttta gcttttggtt acttagatag taatcaattt
     4741 catcaattag acgttgttta tcatcaggta atacaccttt taaattagca taatttgatg
     4801 catgattact tctaaataca attttttcat tagcatctat attctttatc atctctttaa
     4861 tttcttgtaa cacagcctta tcctctagta ttgtaaactc tcctgcctta agtaaatcgt
     4921 aaagttcagt attaggttct accattaaac taagtacacc cacataatct ggtgaaattg
     4981 ctcctaacat ttttcctgta tcaactgcat gctgatgtgt ttttttagtt cctccaagac
     5041 ctgctattac agtggctgat aatttaatcc cacttttttt aacttttcta ccaactttta
     5101 ttaattgttc actactaaaa cctttcttaa tatcttctaa tacttcattg cttccacttt
     5161 caagccctag ataaatcatt ttaacaccaa gttttcttaa ttctttaagt tcatcatcag
     5221 attttttctc tatagattta ggagagccgt acatagtaat tctttcacac tctggaaaaa
     5281 cttcttttat atataaaata atatctctta atttctccat tggtattatc aatgcatctc
     5341 catctgctaa gaatattctt tctacatgct tataatatct tctaaatata tcaatttcat
     5401 tttttattac ttctagtggc tttattttaa attttttaga tttgtacata ctacaaaacg
     5461 tacatttgtt atgagaacat cctagtgtga cttgtattat aagactataa gcctcacttg
     5521 gtggtctgta aagtggcata tcgtattcca tattaaaatc ccccttgtaa taaaatattc
     5581 attctattat attattaata tttactttaa ataataactt ataaaattat attttttaaa
     5641 ttttagttta agatatattc tttaaaaatt taatgatact attggcagta atattaagtt
     5701 gtttttcttg acttataata gcatcattat ctcccttttg ttttccatag tctccaaatt
     5761 gtgcatgatt tcctccttca atctcaacat aagttgtatc atcaggaagt ttttgttttg
     5821 attctacaag acttttaaaa ttgactactc catctttgct cccccatata gatataactt
     5881 tttttcctaa ttctttcaac tcatcaccca ttggataaga agctaaaaat acaacgcctt
     5941 caattaattt attatcacgt gcaaaatttg atgctgcaac tcctcctaat gaatgacctc
     6001 ctattaccca atgttctatg ttgttgtaag aatccataac tttctgtgcc ttattagttc
     6061 caagtatagc tagattaagt ggcatcttta cgattacaac ctcatatcca ctttctgcta
     6121 tttttcttgc aagtggagca taagattcta tttctacttt agctcctgga tacaaaatta
     6181 aacctttaga aacttttctt ccttttggtg taaatgaaat gttttcattt acagttactt
     6241 caactttact attgctgact agagcttctt ttgccagctt ttgaggttca tatgttttat
     6301 ttatccatat gaaaaagcca agcaaaatca atacaaatag tatacctatt cccttaaata
     6361 tcttattctt tatgttaaat tttctattat tatttttatc atctttgttc tcaacctcat
     6421 cataaagtaa aggtctctca agttccatag ttatactaaa tgtatcatat ttcttattac
     6481 gctttacctt tacatcttta ctcataaatc taacccccat aatatttgaa tactattata
     6541 gtttatccta agtaaattgt tttttcaacc aatgaaaaaa atcaatattt taactatttt
     6601 catataaaat tcataaatat atgttttttg ttttttatat attattttct tttagtattt
     6661 tataaaacaa atagccatat actttatttt aaatatatgg ctattctata atttaatatc
     6721 taagctgctt cttttattga aactttagtt atattactta ttctatacat ttctttgaca
     6781 ccatttagag tttgcagtaa gtttagacat attatcttgt cacctagact tttagtattt
     6841 acaatactct ctaaaattac acttcttcct tcatcatact tatctacatt gtaatttgaa
     6901 atataattag tatctctcaa tattttttca acatcttcat cttttatttt atttttatta
     6961 acatttatat atagattata catattactt attagtaatt tctgattttc taaaaaatcc
     7021 tcttgatgtt tatctttata tatagcattt atcaacataa gtctatcttc aataagtgat
     7081 ggtatcaata ataaattttt tatatagtaa acatcagcta cattttctat agacttattg
     7141 acttcatcaa ttatattttt aactattcct ttatacattt ctataacatg ttgatagcct
     7201 gttttagcat catatggtaa tataaactta tttactatta atgcaataat tgctcctaat
     7261 gctacatatg aaatcctgct tatggttaaa acatttgggt ctcccataac tactgctgaa
     7321 ccaagagctg aaacagttac acataccatt aattttctat aatcaactac ataagagttt
     7381 atataccctg ctaaaagaac tattaacgac cttaatgtac tatcttttat tattgaaaat
     7441 aatacaataa atattacagc tcctataaat gtaccttcta ttctttttct agacctttgt
     7501 atacaatttt ctgcgtaagg ttgtgttaat gaaaagacag ttaacattat ccatcttcca
     7561 tctcttaaat ggaaataatc cataataaaa cccgctacag ctgtagctaa agcaatcttt
     7621 actgcataag aaaatcttat agaatttaaa ttaaagtttt tcttatatat tgatagtcgt
     7681 ttaaattcat gaggtatctt tgattttctt tcagctttct tttcgtctct tgattttact
     7741 tttttataat ctactaaaaa ctcatataaa ttttctaaaa cactataaat ttcttttaaa
     7801 tttatatttt tagtatcctg agcaataaca taatctctaa atcctaatat ctctttatca
     7861 gttatggtat ctttattttc taaacacatc ttaatatagt ttatttcttt cgttgctaca
     7921 actagaacat ctgatttact atttatgcta ctttcttcta aaatattttt tatatcttca
     7981 tcttcaaatt ctttactatc ctttttgtat ctttccatga ttaaacttat tctttctaat
     8041 gaaaataaaa tattcattat gtctatacct ctaatactga tataaaagtc atcttttcta
     8101 ttatcaaaaa taaccatttt caaattttta gatatagaat ttattctata attacagcta
     8161 tctatttttc tcatactaga tttaattata gaactatcat cattaatagt attttttaaa
     8221 agactaattt tttctaataa ttcatcacat atagatatca gttctttatc tccgacattt
     8281 ttcatcctat ttctattcat aacaaattga gcaatcatta ttataactgc tccaacagct
     8341 aaagccgaca atctcatagg aaattcactt tttcctacag gtatacttac cataaatata
     8401 tacattagac caaaaggtac atatactgct ctctttaagt cataactaaa tacataccct
     8461 attataaata atgctataaa atttaataaa actcctaaat acatattttg tactgcaaaa
     8521 aatgatagta ttcctaagat tatatttatt gctaaatact tcagtaaatt tttaattgga
     8581 gaaatagtta aatctctttc caagagtgtt agcatagctg ttactatacc tacacctata
     8641 agtgtatttt tacttccaaa taaatatata aatatagaaa taaatgctat tataaaaata
     8701 aacaactttg tttttgatat tactagtttc tttgtcatca tatcccccct aaaaatttca
     8761 acaagaccaa ttttatcata tttgttatct tattcaaaat gcgctttttt acggtaggac
     8821 tttaactttt tttatattta gaactatata attcatatat aaacgtattt tttttatata
     8881 taaccgatat attttcgtat gctcccatat tttttttgtt tcaaatattt tttcaaaaat
     8941 ataaaaataa ctctctcaat aatactaaaa atgtatttat caagagagtt ataatttata
     9001 caataaattt aattaagctt ttctttacct aatatataag ttccaagacc cataaataaa
     9061 gctcctccaa caatatttcc aagtgttaca gctactaaat tataaatagc tcctccaata
     9121 gtaactgtac ttattgttgg tgaaaataat gaaacactat aaattgtcat atttgctaca
     9181 ctatgctcaa aacctgatgt tataaaagca aataaacata agaatatcat aattattttt
     9241 gcagtatcac tattagttct aaacgaacac aacactgata cacatactag aatattacat
     9301 aaaatccctc taaagaaaag agctgtaaat ggcatggatg ctttactagc tgctgtattt
     9361 gcaaaaaatt cagctacagg tcctttgtct actagaccag ttcctacaaa tataagacct
     9421 aaaaccaaag ctccgattaa attacctacc caactgtatg cccatatttt agaagtatct
     9481 tttatactta cacctttatt taacatacca gcagacataa ccatattgtt tccagtaaat
     9541 aactctgttc ccgtcattat aaccaaactt agtgctattg caaatgataa ccccattact
     9601 atcttagtca taggtgaacc tgcatcagtc aaaagtcctc ctatagtaaa tatcaaaagt
     9661 atacctatac ctacataaag tcctgcaaac gcagaagata ccaaatattt tactttactt
     9721 gtgtttaata aatttatctt atttatagct gcattagtta atttatccaa agtttcttta
     9781 tgcataattc cctcttcctt ctattaaatt ttaatttatt caaatatatg ttttactcaa
     9841 tttatagata tttaattcca ctccagtata tgttttcgta cattttagtt atttctggaa
     9901 gctctacacc ttcaagtgcc cattttttaa attccataaa tcctgttcta tcaattatat
     9961 atcctatatg ttcttttcct cctggtgcat ctttatctat atacttttct acatatctat
    10021 aagtattaag tattatcttt attatgctgt cctcatctac ccataaaaga aaatcttctg
    10081 ctagtctagg gtttttcttt cctgttctac ccattatagc taatctatag tattttttct
    10141 catctctagt ccatgcattt gttgggcaat ttagtacaca ctctccacat cctatacact
    10201 tatctttatc tcttaccacc ttattatttt ctgtttttaa tgctccaaca gatattttag
    10261 aacatttttt tacacatgct ccacaattta cacatctatc tttttcatat attggtaaag
    10321 ccataccaat aattccaaag tcattcattc tagcttttat acaatcattt ggacaaccag
    10381 ttaaagccac tttaaaatgt aaatcatttg gaaatatagc tttttctatt cttttagcaa
    10441 attcagttgt attatattga gcctttggac agactttatt tcctatacaa gctgctatat
    10501 ttcttgtacc tgctgctgga taacctgcgt ttttctcact ttgatttata ttcatttttt
    10561 caataacagg ttgtataatt ttatttattt cttccatatc ttccatattt attcctaata
    10621 tttcaaaccc ctgtcttgta gttatgtgca cctgtccatt tccgtactca cttgcaattt
    10681 tagaaactac agctaaactc tcagggtcaa taagaccacc aggtactcta actcttaaag
    10741 aagtctcata ttttgttttt gtaattctat aagcattttt cattactttt ttggtattta
    10801 aatctcttat cacaaacttc gccccctaat caataagatt tttagcatat gaataatcaa
    10861 atactggtcc atctaaacat atgtaagtat catccatttt acaatgtcca catttaccta
    10921 ttccacaaca catttttctt tcataagaaa cccaaatgtt cttttcatct aaatctcttt
    10981 ttaaaaattc ttctactgta aatttcatca tcattggagg tccaactaca actatagaag
    11041 tattgtttat atctttcata tccaattctg gtatgtattt tgttactaat cctacattac
    11101 cactatatcc ttcctctgct ccatctacag taactaaaat atcaagtttt ttactccatc
    11161 tctttaaatc atctgcaaat aaaatatctt ttggtgattt aaatcctact attaatttaa
    11221 acttttcgca tttttcttta ttattataaa aatattcaac tattcctctt acaggggcta
    11281 atgcacttcc tcctgccact actacaattt ctcttccttc ataaagggat acatcaaatc
    11341 catttccata aggacctctt ataaaaaaag aatctccttc tttataatta aacaactcgc
    11401 tagttacttt tcctacattt cttattgtaa agtcaatata atcctctcca tatcctgata
    11461 ctgatattgg actttcaccg tatttaggta tagatatctc ataaaacttt ccaggcaata
    11521 caccttttgt attgcagttt actctaaaag tccattcttt atcagtatgt ttaactatat
    11581 ctagtatttt tgcacttact ggtatatatg ggttcattta tacttcctcc ttactaatta
    11641 ctaaatcatt tattttttct atacactttg aaaatgatat gtattgcgga caggcatcat
    11701 cacatcttcc acaaccaaca cacatatgat atccgaatct ttttttaaaa tcatgtattt
    11761 tatgcatagt tttaaatctc attctttcac cttgtttttt tctaaaagaa tttccacctg
    11821 ctatatctgt atatccatct acttgacaag aagcccaaac ccttcttctc tcacctacat
    11881 tttcgttttc cttatagaaa atatcctgca ttgtaaaaca tgaacatgtt ggacatacaa
    11941 aattacatct accacatcct atacatcttg tatcatattc atcccatatt gaatttccta
    12001 taatttcatt tgaatcaata ttctctggca catctacaga tattaaatta tcagttacat
    12061 aatcaacatt aaattcagtt gcttctccat taaatacttc aaattcacta tcctttatat
    12121 ctaagtacag tgtatctcct tctatattta atccaattga ataattatca gttttgttag
    12181 aatccatgct cacacaaaaa cagtttctaa aactttcctt acaccctaca agtgcaaact
    12241 ttactttatc tctgatattt ttataaaaat aatctttttc ttcaccatta tttagatata
    12301 tttcatcaag tctttttata ccatttatgt cacaagctct taagaaaatt aaaattttct
    12361 tgttgtctaa atcgctttcc ttaaactctt tttcagtaaa ataaaacaat acttgattta
    12421 tgggaagtac aacttctttt ggtgaaaagt ttgctttctt agcaaattcc atttcttcaa
    12481 aagcgtttac ctccttgtat cttataacat cagtatctga aaaagtacct ttaaatggga
    12541 ttaaaactgg tgcaaatatc ttgtagtctt tttttagata attcagacct tcattgaact
    12601 tagatgaatc taattttagt ttcattttta tcacctttct tatattttag ttttacttac
    12661 ttgctatctt gtttaaatct taacacttta ttttctatgc ttctgtgata attatcacag
    12721 tttcaaaaat aataaaaaaa tattaataga gtttactaat ctctgttaat atttttttat
    12781 tatctatatt attcgatttc aaatctaatc acttagatac aatcattaaa tacaataaaa
    12841 ctattatcta ctcaattttt tgaataataa agaatcttat ttgctttttt atacaattca
    12901 atagggtaaa ataaaaatta ataaattaga caataaaaaa gaatctataa atagattctc
    12961 ttaataatat ttttatttaa ttaaaattct aataatactt ttacttaatc ataattaatt
    13021 atttttgaaa taccttgata attcagtttc tttaacataa aaacctttat tatcaatctt
    13081 aataagttct tctttttgca atacctttat cattctggaa acagtctccc ttttacagcc
    13141 aagcatatca gctaaatatg taactgtaat atttacattt agtaaagtcc attctccaca
    13201 gcaaactcca aattctctac ttaacctata taattttgct gcaagttttt tatctacttt
    13261 tatggatata gaatttttca actgtctata taaacgtctt attcttcttt ccatataaga
    13321 aataatatcc tttgtaagtt gaaaatcatt ttgcataatt tctaaaaatt cttcaatact
    13381 ataataagct acaattgaat tttcaaatgc ttcacagcca acagtggagt ttttagaaga
    13441 gtcaaatgta atatcattta tgacttcccc actatttaat atgaaaatta cttttctctc
    13501 tccattttca cttatcttaa aaatagatac ctttccttct aaaagtatat aaacactgtc
    13561 taagctatct ttttcataaa ataaaatttc ttttttttgt aatcttttta gtttagcttt
    13621 ttctttaagt tgattcttcg ttttagtact taaattatta aaaattatta ctttatcaat
    13681 attataatct tttaatttag acatgtaata tctctccatt cctaaatttt taagagagct
    13741 ctataaaaca ttacaaattt cttattttat tcatttttaa acaattttat agcatttgta
    13801 gttttaattt tcctttcaat agcttcatta gaaagttctt ttactacctg tgtatatact
    13861 aaatcaataa tgaaaaattg tgctaattta gaagatattg aaccagtttc cagtactgtt
    13921 tctcctgata cataagataa gtgtacatct gaaatatcct ttaattcaga atttttattt
    13981 tcagtaattg atataatttt agcattattg gctcttgcaa gctttacagt ttttataatc
    14041 tcttctgttt caccagaatg tgaaattgca attattaaat ctccttcttc cattatggaa
    14101 gacatcatta tcatcgtatg actgctatca aaacttacac aattaaggcc tattctcata
    14161 aatttatagt ttgaatcctg tgctattata ccagaatatc caatgcctat gaagtaaact
    14221 ttctttgcat ttattataag ccttgcacag tcatgtacat ccttactatt gattatctct
    14281 actgtgttct ctaaagtggt tacatttgaa cttaaaagtt tttttgctgt atccaaagct
    14341 ggctcatcat tttgtatatt tttatttata atattttttt tattgatagt tgatatttct
    14401 tgtgctaaag taactttaaa atcctgtaat ccattaaaat tcattttttt tacaaaccta
    14461 gttatagttg cttcaccaat attgctctct ttagctattt gtgatattgg tttataaaat
    14521 acatcatcta tattttcttt tatataagct attaataatt tatctgattt tgtgacttta
    14581 aaagttggtg attccaattg ttctaaaata ctcattaaaa acccttctat tctttatatt
    14641 ttgtttaaaa aattatataa tgctccaagc attcctgcat cattttgtct ctttgcaaat
    14701 tctatattag tgtttttgta aactctctct ataagaacct cctttaatgc tttgtctatt
    14761 tttggtttta ggaacttttc ttgtgccata atcccaccac ctaaaattac cacttctgga
    14821 tttattacat aacatatatt tgcgattcca actgctaatg attttatcat attatcgagt
    14881 tcttttaaac aatcttcatc attattcttt gctaaatcaa aaattatttt tccatttaag
    14941 tccttttcat tcaaattttt tatttttgct acttttttaa ttagactgct agtagatgcc
    15001 aattcttgaa aagaacttcc atttatattc atatatccta cttcaccagc gctattacta
    15061 aacccattta tcagtttatt gtttattata atacatccac caattccagt acctattgtt
    15121 aaacatatag aagatgttgc acctctacct gcgccaagcc acacttcacc aagtccagca
    15181 caattaacat cattttcaac ttcacatttt atattaaact ctttttctac ttcttttttt
    15241 aattgcatac ccttataatt tggaattaat tcttctaatg caaatagtat tttaccttca
    15301 actgggtcaa ccatacctgc agttgatata catataccac ttatttcatt ttcctctata
    15361 tattttttta ctatatcttt tattttggct aatattcctt ctccaccttt ttcctttgct
    15421 tcagtatcaa gacttgattt atcaattata aatccatctt ctctaataaa accatatttt
    15481 attgaagtac ctccaacatc tatacaaacg taatttttca taacatatct ccatacaaat
    15541 tatttttata aattaacaac tctatttaaa attttatatt acacaaatct tttacttatc
    15601 ttttccaatt cacagatata tttccttgct tctgataagt ctatagtatc acttaataca
    15661 cttttttctt tggttatatg atacttataa atatttttac ataagtcctc tcctaacata
    15721 ttaagacctt cttgagtgta atgaacatca tcaatagtta atcctctgtc atgaaattgc
    15781 atagcaattt tacttcctac acacagtata tcacattcat tacaagcaga ttcttgagct
    15841 gctgcaattc gtcttgttct aataaaatat tcatcttctc caagccaata accagttaaa
    15901 cttacaaaca ttttatcaat catacattct tctttaagcc tatacgcaat tttttttaga
    15961 ctttctttat agtacaatgc atcactacca tattttgcat cactttcacc ttgaatccaa
    16021 ataacgtatt tattttctat attataatgt ttacttaagg tagtacagcc tgtattaaat
    16081 ttttctatag tgtcatttag aaaccagtta ttatcatgat tccagttttt tattcttgaa
    16141 ccatcttttg cattgtgaat aaaacaaact ttatttccag taagcttatt ccattcattt
    16201 gaaaatgcag gtgctatagt accatcacct tctgaagtct ctagctcaca tctcatattt
    16261 ataatttcat cagtactcaa atattcaaaa caattttcat ttggaatgat gctctctttt
    16321 ggatttccca atcctctagc attgctttgt cctattaata agaatacatc tatattttcc
    16381 ataagttaac tcctttacaa aattttaagc ttttaattat tttcaatttt ctcataaaaa
    16441 actaaggctg aaaatagtaa tctaactaaa atttacgaag tctcactaat cgctgaaagt
    16501 taaatcacta ttttcagccc tagtggttat ataaattatc attatctaac caaaacctta
    16561 aaaatagctt ttttgatatt ttctttttct aaaactttta ttcctgtcat atgagcatct
    16621 tctggataac aaactaaaaa atcacctttt ctaagttcca tgtatccatt atttttacca
    16681 tcaagaggca agaaatcgcc cttttcttca tatatttttt gttctaaatt ttctataaaa
    16741 tttatatcta ttcgttcaca tccatcaagc atcaaatgaa catctatata cttcttatga
    16801 gcttcccaga atctatcttc tttttcacaa gttttatact ctacaatatt cacaaaaata
    16861 tcatcaccat ctattgtata agtacctttt tcataatcta ttaatgaatt atctttagca
    16921 tattcaaaac atctaagtaa atcttcatgc aatgttgaat atgtcttact atgatttata
    16981 tttccaaata tcataagcac tatctcccta tataattata aagtctattt ttatactttt
    17041 atatacaaag tatattatac aatcttatag agtaactaaa ttatttatct aatcactcta
    17101 taagacaatt tatcatttaa atattatttt tcataataaa cagtagtctc agcttttggt
    17161 aaagatactt ttgctttaac tttagcttct attgcactaa ctattgtacc taccactaca
    17221 gaagtcgtga ttgttattag agaatatgcc caagaagtta cttctggcat acggtatttt
    17281 aaataaacaa ctaaaattgc tgatactatg aatccaacat atgctcccat tttagtcgct
    17341 tttttacaaa atgcaccaag tacaaatatt cctgctaaaa cacctagtac tagccccata
    17401 aaactattaa accattcata tgctgacttt atttctccat ttgctaatac tattgcaaca
    17461 gctatagaaa gtatacctac acctaatgat atatattgtg ctatcttagt ttgcttttcc
    17521 atacttatat cttttgttat gatagtttgt atatctaaag tccaacttgt agcaactgaa
    17581 ttaagccctg tcgataaagt tgattgagaa gctgcatata tcgctgcaag tagtattcct
    17641 gttataccca ctggtaattc ataagctata tatgatgcaa atacttggtc ttgttgaact
    17701 gtttgagcta aatctgggtt ttgagtatag tatataaata aacaagttcc aattagataa
    17761 aatactgttg caagtcccat tgataaaaca ccatttccat atgtcatttt attaagttgc
    17821 tttatatcag ttgttgtagt aaatctttga actatatctt gactcgatac atatgatgaa
    17881 aaagtattaa gaccagctcc aataaatatt ataaatgcac ttgtagatag tatattttta
    17941 tcaaacataa cttcattact tgctaagaac ttgtgtccag ttgtaagagt atccattact
    18001 gttccaaaac caccatttat acttgctatt aaatagaaca atgtccctgt aacacctact
    18061 attaatacca taccttgtat aaaatctgtc cacaagacag cctttaatcc acctgtatat
    18121 gaataaatta ttgctattac acccataact actatcaata catttacagg tattcctgta
    18181 agaccagcta gaaccactga tggaagatac attatgatag acatacgtcc tatttgatat
    18241 attataaaca tcaaagctcc aagtactcta agacctttac tttcgtatct catttctaag
    18301 tattcatatg ctgtatctat ttctaaccta ctatacagag gtaagaaaaa ttttattgtc
    18361 aaaggtattg ctataaacat acctaattgt gcaaaccaca aaatccaagt accaccataa
    18421 gaatttcctg ctaatgataa aaatgatatt ggacttaata atgtggcaaa tattgatact
    18481 gatgtaaccc accacggaat agttccatca cctttgaaga actcttttcc cttcatttcc
    18541 ttttttgaaa ataacaatcc tgcaaataaa actgccaata agtaaaccac tagtattgca
    18601 aaatctatcc aagtaaatcc taccatttga ctaatcctcc catactaaaa gtattttatt
    18661 aaaaatactt tctgtatatt tcttttgctc ttgatttcat ttcatcagtt gcttctttca
    18721 taggttgtct acagtatcca gcctcaacac cttgttcttc aagtagtaat ttaattgttt
    18781 gatataagcc atttcctaat atatctgtta ttaaatcatt agtcacatgt tgtacttcaa
    18841 gtgcttctga tattttttca ttcttagtta attcgaatat ttgtctagct ctgacaccgt
    18901 ttacattgaa tgtagaacca attgccccat ctacacctaa aactgtagct ggtaacatca
    18961 tttcatcaaa tcctgcaaat attaatttat ttgggaatgt ttttctcatt ctctctaata
    19021 agtagaaatc tgctgctgta aacttaacac ctattatttt ttcattttca aataattctc
    19081 caaactggtc taaactcata tcaacaccag ttaaaaatgg tatagagtat attattaatc
    19141 tattatctac agaatttatt attgtattat aatagtgttt tatttcttca aaatcaaatt
    19201 tatagtaaaa aggtgtaaca gcacttattg catcatatcc taaatcagtt gtaaatttag
    19261 caagttctac agcttctttt aaattaactg aaccaacttg agctattaat tttatttctt
    19321 ctttcacttc atctttagct atttcaaata ttcttttctt ttcatcagtt gaaagcatga
    19381 agttttcccc tgtacttcct cctacataaa gaccatctac tttacaaaca tctatattgt
    19441 gtctaattat ttgtcttaac cctttttcat ttatattccc ttccttatca aatgaaacta
    19501 ataatgctga ataaattccc ttcatatctg ttgttctcat atctttacct ccaattttaa
    19561 ttttcaagta tagttaaata taataatttt ttaatttata aattaatttt taattaattt
    19621 caattgcttt aacaaatttt tctgttatta attgtggtct tgttattgct gaacctacta
    19681 ctgatgaatg aacacccaac tcaaagacct tttttaaatc ttgaggagta tttacctttc
    19741 cttcagctat tacaggtatt tctaaatctt taactaatct ttccattaat acaaaatctg
    19801 gaccttctaa tgttttagta tatggagtat atccagatag tgtagttgaa acgcaatcta
    19861 caccgtactc ttgtgctttg atagcctcat cataatttga aatgtctgcc attactagaa
    19921 caccgttttc ttttatatac tttataagtt cttttaagtc ttcattattt ggtctaactc
    19981 tattggtagc atctaatgct atcatttcac accctgttgt taaaagttca tcaacttctt
    20041 ttttagttgg cgttatatat atatctgaat cttcatagtt tctctttata atacctataa
    20101 caggaagtcc agtaactttt tttatttcaa taatatcttc aactccttga gctctaattc
    20161 caacagctcc accttccata gcagccttag ccattctccc cattataaaa ggactatgaa
    20221 gaggttcatt ttctaaagct tggcatgata ctattaatct tccctttacc ttatctagca
    20281 ttttttaaat tacctcgttt ctgaaattta ttttcttgat ttatgtatta ataataacat
    20341 gaaaactttt tctttgcaat attttttcat atttttttct cgcatctata tttttactga
    20401 tttattttat atattagctt gttttttaca tttaaaagaa attttatttc atttttttgg
    20461 tgtttatttt cattttatat aactactgtt ataagtccat ttaaataatt tcaataaaaa
    20521 aacccatgta tactatacat aggtttccca atattattct aaatccatta taatttttct
    20581 tccacagcat ggacaaaatt cagcctctgt atctatctca ctttcacaat aaccacaaat
    20641 tataaaatca tcacattcat tttccaattc aaatttttta ccacattcag cacaatattt
    20701 atcatttaca tttccaacat aaccacactc acaagttaca ttactttctg ccatttccat
    20761 ttcattaatt tccatatttt tttcataaat taacttatca agttctagta actcatcaca
    20821 gaatttatca aaactattat cattaataga atcttctcta attttttgat atgtcagtat
    20881 tcccatttcc aagaatatct ttgctttctt ttcttgaata tcatcaatct ctctatttaa
    20941 ttttgatttt tctttatata attcaacttt aaattttcca ctaccaaact ttttcttaac
    21001 cttagaaaat ttatcatcta ctttattatt agtatttcct tccacagaaa ttccctccaa
    21061 tcatatttaa tcagctgaat ttagcttata tcctattaaa gatatcacaa aactatatac
    21121 aaatgaaatt acaatagctt gtaagacttt aaattgcatt attaaagttg ttccataatt
    21181 agttgtatta aagaaattaa tattactatc tagtataaat gtactaaata aagccaatat
    21241 tcccatcaaa aatgcataat atatactaaa tactattata ggtcttatac tatctgtatt
    21301 atacttaaat cttagcttgt atccatttaa taataatatt aacatagaca atgctcccat
    21361 tgcataaaac attaatttag tatctccaaa taaagatgaa gaacttaaag taaacattga
    21421 tactgttgag tttattatgg ttactgaaat tccatttgca aaagcccaca tataactagc
    21481 tatttgtggg agtatagtaa ataaatttaa tccatttgaa taactactca tatctagttc
    21541 atttaagtaa ctactatcag atatagttag taccaatagt attactaata ctaaaacata
    21601 acctaatatg aatgttctta tagaatttga aaaaagagat aaatacatat tttccttttc
    21661 ataacttttt ttataagtag ttatgtatgt acatataaac cctaatacaa atccatttaa
    21721 aagaacacta aaaaattcat aactatattc taatgcatat ccatattcta acatactatg
    21781 tgaactagtt tttacattag taaaaataga taaaatagct aaaactaatc cataaaaaat
    21841 tccaactcct agagaatttg ctaatacagt ttttgaatct ctacatcttt ttctcataaa
    21901 aattaggttt gatattaata ataccaaaat agggattata agtaatatca acaaacctat
    21961 attggcattt ataaacccag aacccatcat ggtagacata gatacactaa tttgacccaa
    22021 gttgagagct aggattatat gtgctggatt tattaaataa ctaatatcat taaattgaat
    22081 agttataata gctttaaata tcaatgcaat aataaacaat ataaatatgg ttgtaaatgt
    22141 tgttaaaaat acacctcttt tatttgcatg atatgaaagc tcttttattt tagatttcaa
    22201 gtctagtttt gtttcaagtg gtcttaatga tgctatttca taaagttcat ttccacaaaa
    22261 tttacagtaa ttatccttag tattattttc tttattgcaa ctagaacaaa ttttttcttc
    22321 tttactttta agagtgatat ggctattttt tatttttcta gttggttcac ctgtcttttt
    22381 ggaataatta ttatgtgaat aattcatatt atttattttc ttttcaccca ttttttccct
    22441 ccaagtttta ctattgtaaa ttatcaaata taactatatc atataaaact taattatacc
    22501 ttaattggat atatt
//
LOCUS       DAGQWR010000065        22338 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.065, whole genome shotgun sequence.
ACCESSION   DAGQWR010000065 DAGQWR010000000
VERSION     DAGQWR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22338
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.065"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            108..899
                     /locus_tag="MIW95_002108"
     CDS             108..899
                     /locus_tag="MIW95_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBY3151433.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            928..2148
                     /locus_tag="MIW95_002109"
     CDS             928..2148
                     /locus_tag="MIW95_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBY3151434.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2168..2863
                     /gene="pxpB"
                     /locus_tag="MIW95_002110"
     CDS             2168..2863
                     /gene="pxpB"
                     /locus_tag="MIW95_002110"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBY3151435.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            2893..3879
                     /locus_tag="MIW95_002111"
     CDS             2893..3879
                     /locus_tag="MIW95_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBY3151436.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRSKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLNSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4098..4664)
                     /locus_tag="MIW95_002112"
     CDS             complement(4098..4664)
                     /locus_tag="MIW95_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3151437.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            5016..6083
                     /locus_tag="MIW95_002113"
     CDS             5016..6083
                     /locus_tag="MIW95_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBY3151438.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEESKDISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6102..7262
                     /locus_tag="MIW95_002114"
     CDS             6102..7262
                     /locus_tag="MIW95_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBY3151439.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEETELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            7275..9371
                     /locus_tag="MIW95_002115"
     CDS             7275..9371
                     /locus_tag="MIW95_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBY3151440.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGFNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTKEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(9530..9874)
                     /locus_tag="MIW95_002116"
     CDS             complement(9530..9874)
                     /locus_tag="MIW95_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBY3151441.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            10166..11056
                     /locus_tag="MIW95_002117"
     CDS             10166..11056
                     /locus_tag="MIW95_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBY3151442.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            11274..12560
                     /locus_tag="MIW95_002118"
     CDS             11274..12560
                     /locus_tag="MIW95_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBY3151443.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDICEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKKFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(12649..13092)
                     /locus_tag="MIW95_002119"
     CDS             complement(12649..13092)
                     /locus_tag="MIW95_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBY3151444.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(13116..13910)
                     /locus_tag="MIW95_002120"
     CDS             complement(13116..13910)
                     /locus_tag="MIW95_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBY3151445.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(14476..14952)
                     /locus_tag="MIW95_002121"
     CDS             complement(14476..14952)
                     /locus_tag="MIW95_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBY3151446.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPNVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(15053..16222)
                     /locus_tag="MIW95_002122"
     CDS             complement(15053..16222)
                     /locus_tag="MIW95_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY3151447.1"
                     /translation="MLNIRTLCHEINDWIINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKNYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            16385..17248
                     /locus_tag="MIW95_002123"
     CDS             16385..17248
                     /locus_tag="MIW95_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBY3151448.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDDLYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            17319..17912
                     /locus_tag="MIW95_002124"
     CDS             17319..17912
                     /locus_tag="MIW95_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBY3151449.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(18163..18798)
                     /locus_tag="MIW95_002125"
     CDS             complement(18163..18798)
                     /locus_tag="MIW95_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBY3151450.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNIHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(18878..19609)
                     /locus_tag="MIW95_002126"
     CDS             complement(18878..19609)
                     /locus_tag="MIW95_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBY3151451.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLYMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQNNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            19940..22129
                     /locus_tag="MIW95_002127"
     CDS             19940..22129
                     /locus_tag="MIW95_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBY3151452.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEYNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKKALPE"
BASE COUNT         8543 a         2557 c         3751 g         7487 t
ORIGIN      
        1 aattgaatac aagtgataaa atatttcatg caatatataa aataaacaaa atttaaaaat
       61 taaaactact tattctatca aaatgttagc aaggaggggg tcataaattg tataaagtag
      121 atttaaatag tgatttagga gaaagttttg gaacgtataa gattggttta gatgaagaag
      181 ttttaaaata tatttcatca gctaatatag cttgtgggtt tcatgcaggt gaccctagtc
      241 atatggaaaa aacagtacag ctagctaaaa aaaatggagt gaaaataggc gcacatccag
      301 gtttcctaga tttaatagga tttggaagac gtgagatgaa aataacaaag caggaagcta
      361 aagattatac aaaatatcaa ttgggtgctt taatggcatt tgcaagttca aatggatgta
      421 atatacaaca tgtaaaacca catggagcat tatacaatat ggcagctaaa gataaagagt
      481 tagctatggg gatttgtgaa gctatatatg aagtcgataa agatattata ctgttggggc
      541 tatataatag tgaaatgata aattctgcta aagaaattgg acttaggttt gcaaatgaag
      601 tatttgcaga tagagcttat gataataatg gttttttggt tccaagaagt gtagaaggag
      661 cagttataca tgatacaaaa catgcaatag atagggttgt taggatggta aaagaaggaa
      721 ctgtagaaac tttaacaggt gaagttatac atatcaaagc agattctata tgtgtacatg
      781 gggacaatcc aaaagcaata gagtttgtca aagaaataag aaagcgtttt gaattagaag
      841 gtatagaagt ttgtccatta gaaaacatag aagtttgttc gtcagagaac atagtataag
      901 actgataaca tgagggggag ataaaatatg agtacaaaaa atattacaga taaaactaaa
      961 aataaaaaag atgtcggagc acttataggg gctgcattta taatggcaac atcagcaatt
     1021 ggaccagggt ttttaactca gactgcacaa tttacccaag attttggacc aaatttttca
     1081 tttgtaattt taattacaac aatccttttt ataggggctc aagtaaatgt atggagagta
     1141 attggtgtat caggacttag aggtcaggat atagcaaata aaataatacc gggattaggt
     1201 tatttagtag cttttttagt agctcttgga ggattagctt ttaatatagg aaatgttggt
     1261 ggagctgcac ttggtatgaa tgtaatgttt aatatgaata tgacattagg aactgtatta
     1321 agtggattaa tagctatatt tgtatttatg tcaaaaaatt caaattctct agtcgataag
     1381 ataactaaat ttttagcatt aggaatgata attatagttg gatatgtagc aatatcaaat
     1441 catcctcctg ttggagaggc agtatcaaga atggtaaaac cagaaaatcc aaaaggattg
     1501 atattcccaa taataactct acttggagga agtgttggag gttatataac ttttgctgga
     1561 ggacatagat taatagacgg tggaataaca ggagaagaaa atataaaaga aataactaaa
     1621 tcttctcttt tgggtatatt agttgccaca atgatgagag ttcttttatt tttagcaata
     1681 ctggcagtgg tttcaaaagg tcttcaactt gacccagaaa atccagctgc ttcagctttt
     1741 aaatttagtg caggagcaat aggatataaa ttctttggac ttgtattatg gtctgctgca
     1801 attacatcag ttataggagc tgcttataca tcagtttctt tcttaaagac actaaatcct
     1861 tttatagata aatatgaaaa atattttata atagcattta tagcaatatc aacacttata
     1921 atggcattta taggaaaacc agctacttta ttaattttag caggagcttt aaatggattg
     1981 atacttccaa tcacattagg aattatgctt atagcatcta aaagaaaaga tattgttgga
     2041 gattacaagc atcctacatg gcttttaata tttggattaa tagtagtttt aatatctgca
     2101 tatacaggaa taacttctct tagcagccta ggtgctttat ttgcttaatt tagaagaggt
     2161 gacatttatg gaaactagat acttgatatc aggggataag gcagtggttg ctgaatttgg
     2221 aaatgagata tcagaagata taaacaaaaa agtaatttca tttatgagag caatagaaat
     2281 atcgaatctt aaaggtattg taacagaaat ggtaccaaca tataggtcac ttatgataag
     2341 ttataatcca ttagaaatag attttgatag tttgattgag aatttaaaaa aaatagaaga
     2401 taatttggaa agtatagtat taccaaaacc taaaatacat gagattccag tatgttatga
     2461 tgaggttttt ggtatagata ttaaaaacgt tgcaagctac aataatctca ctgtagatga
     2521 agttataaaa atacacacaa gcagagagta cttgatatat atgctagggt ttactcctgg
     2581 atttccatat ctaggtggta tggatgaaag aatagctaca cctagattag aagtgccaag
     2641 gactaaaatt tatggtggga gtgttggaat agcaggttcc cagactggtg tatatcctat
     2701 tgatagtcct ggtggatggc aaataattgg taggacacct ttaaaattat atgatgaaaa
     2761 tagagaagaa cagattttgc ttagagctgg agactttata aaatttgttc caataacatt
     2821 agatgaattt atagaaatcg aaaaaaataa cttaagtatt taaatatcgt tatagtacag
     2881 ggagtggtat atatgggatt caaagttgaa ttaggaggtt tccaaacatt aatacaagac
     2941 agaggtagag ttggctatgg tcaatatggt gtatctggat gtggagcaat ggatgagtat
     3001 gcacatagag taggaaatat attagtagga aattctgaag atgaggcttc tttagaagtt
     3061 ttaatgttag gtcctacaat tacctttgat gaatatacgc aaatagcagt aactggggga
     3121 gatttagggg ctaaaataaa tggaaaagaa attcaaaact ggaggtcata tcaaataaat
     3181 ccaggtgatg tattatcatt tagaggtgtg aaatcaggtg ctagagccta tgtgtcaata
     3241 gctggtggaa tagatgttcc acttgcaatg ggaagtaagt caacttatac aagatctaaa
     3301 attggcggat ttgaaggtag agcacttaaa aaaggagatt atataaatac ttttattcag
     3361 gaaaaagatt ttacaataaa taaaaaatta aattcaaaat atattccaac ttatagttca
     3421 gaaatcgtac taagaattgt aaaaggtcct caatttgatg ctttttctaa cggtgaagta
     3481 gagaaatttt tatcaaataa atacaaagtt acgaatgaaa tagatagaat gggttgtaga
     3541 ctagatggag agagtataaa gcaccttaat ggagcagata ttatatctga tggtatttct
     3601 tatggagcta tacaagtacc tggtcatgga aaaccgatta taatgttatc agatagacag
     3661 actagtggtg gctatactaa gatagggaac gttataagtg tagatttgta taagttagca
     3721 caagcaaagc caaatgatgt agtaaaattt gaacttgtag atatatatga agcacataga
     3781 ctattgagag agcaagaaga taagattcaa gatatctaca agagtatgaa aaatataaga
     3841 gttgtaaagg caaaagtatt aaatgatata gcagtttaat ttaattttaa caaatacaat
     3901 ttttattata atttataata taacttacaa atagctattt actatttacc ataattaaga
     3961 tggtttagat tagtcaaata gctatttttt tagtttgagt aaatatttta aatttaaata
     4021 aatacttcaa ttttaaaata aaagcaatat ttaaatttat tatattatct acaaaaagta
     4081 ggtgtgttat gttgagttta tttagatttt attggccaaa ttggaggtga tataactcca
     4141 tatccacgag ctattgtatc tttacttgga ttgatgattt tatgtggtac attagctttt
     4201 ataaatgttg agtcacctgg atttaatcta actacctttt cacccataca aatatcaacc
     4261 acaccactcg ttaccaccat aatttcttct gatgcatgat agatataatc tttagcatcc
     4321 tcactttctg gtttaagttc aaactctaca aataaaattg aaggtgtaaa ttcagttgta
     4381 ggcattggag atactatctc gtaaaacatt tcactctttg gaaatttcat cataactcta
     4441 tcttcttttc taattactaa atcatcagta ttattgtcat ccataaacat atataaagga
     4501 gtgtctaaaa cttcagcgat ttttctcaat gaagacaagg atggttcaat agcatctctt
     4561 tctatttgag agatataact tatagataaa tctgtcaatt tggaaacatc acttaatgtt
     4621 tttttcttgc tttttcttat atttctcatc ttttcaccta gcataagaac ctccaaataa
     4681 taaaattaat ataaaatatt cgcttaaaac aaagtaatta tagcacaaca tagaagtagt
     4741 ggcaataaga atacatagta gattttgaca attaaaattt aattaaatcc aaataaaagt
     4801 ctatgaaaat gaattattgt taaattaata taaagaaaat aataataaaa aaatttaata
     4861 gaagagtatt aattgaatta agattatcac attatatttt ggaaaagagt aaattttcag
     4921 taaattatct tgacatatat attgaagtaa taatataatt taaagtataa aattttaaaa
     4981 attgaattat aacttcaaaa aataaggagg gtaatatgaa gataacagat atcaaatttg
     5041 aaaagcttag aattaaatta aaaaaacctg tagtagtttc atttggagta attgaatatg
     5101 gagaaagtgt aattttaaaa attgaaactg atgaagggta ttgtggattt ggcgaagcag
     5161 cacctcttgc agcagttaca ggtgaagttt tggataatgt tctttcagta ttgcttatgt
     5221 ttaaaaaaga gttaataggc aaagacccac tagatattga aaccatacat acaatcatgg
     5281 atggaattat tataggtaat acttctgcaa aagcagctat tgacatagct ttatatgata
     5341 ttaaaggaaa aattatgaat gcacctcttt ataaagttct tggaggattt gatagtaaag
     5401 tccaaacgga tattactata agtatagata agccagaaaa aatggcagta gaagctttag
     5461 aaagagtaaa agaaggtttt aggatattga agcttaaagc aggtataaat cctgaagacg
     5521 atattgaagc tgtaaaatta attagagaag ccgttggaga gagtataaga attagaattg
     5581 atgcaaatca aggatggaac gtaaatagca gtataaatac tatcaaaaaa ttagaagaat
     5641 ttaatgttga tgcaattgaa caagcacttc cacattggga tttagatggt actgcttaca
     5701 taagaaataa aagtaataca aaaattatga tagatgaatc gttacattca ccaatagatg
     5761 caattaaggc tataaaaaag aatgcagttg atacatttaa tattaagctg atgaaatcaa
     5821 gtggaatata tcctgctata aaaatcaaca acatagctga ggcaagtgga gtaaattgta
     5881 tgttaggatg tatgctggaa acgagaattg gtataacagc agcagcaaat ttaatagcat
     5941 ctaaaaagaa cataacagaa gctgaccttg atagttttat gttttgtgaa gaatcaaaag
     6001 atatatctgg aggatttgta atggatggag atattatgaa tcttgtcaat aaaccaggtt
     6061 taggaataga agtaaactta tgataaattt ggaggttaaa tatgaataca cttttttcaa
     6121 atccaggtag tatacttgct gtaatgacat caatgattgc attgggtttt tacttacaaa
     6181 gatacaaagc cattaaaagc ttaggaccag cacttacaat aatcataatg ggaataatac
     6241 tttcaaactt aaaagtagtt ccagtaagta ctgagttata tggaactatt tcaacatatg
     6301 ctatcccagt ttctatgact ataatgttga tgagtgttga cttaaaagaa atgacaaaat
     6361 tatcaagaga acctttaatt gcaatttttg tagctgtatt aactgtaagt atcatggcat
     6421 ttttatttgg attagtattt gcagaaaaaa tatcagaagg ttggaaggtt gctggaatgt
     6481 ttgtaggtac atacactggt ggaagtgcta atcttacagc tattggaaca ggccttaatg
     6541 taagtagaca aactcttgct gcagcaaatg cagcagacta tgtaattgga gtaccaacat
     6601 tgatatttat gtttgctcta ccagcaatat taaaaaattc aaagaagttt aaaaaacttt
     6661 ggccatatca tgtagaagaa acagaattag aagattgtca aaatgaagaa tttatggagt
     6721 caaaagaatg gagtattaaa gatatagctt ggatgttggc tataggtttt gttgttacag
     6781 aagtttcaac aatacttgct ggatatttca attcaagttt tagtagtgca gcaagaatac
     6841 ttttagttac aacaatttca attataattg ctcaattaaa gccagtaaag aaactaaagg
     6901 gaaatttaga tttaggatta tttgttgcat tattcttttt atgtacaata ggtttttcag
     6961 tagatataaa agagttttta ggttcaactt ttactataac actttactgt tttagcataa
     7021 tttttgcttc atttgttttt catttaggca taactagact tttaaagata aagtatcaat
     7081 atgtaatact ttctatagta ggagcaattg ctgatggtcc tacttcagct ttagtagctg
     7141 cttctgcaaa gtggaattcg cttgtaagtg tagcagtcgt aatgggagta attggtggag
     7201 tattaggaaa ttatgcaggt atatcagtgg catatgcaat aaagatgtct ctaggattat
     7261 aggggggagt ttaattgata aaatttaaat tatacatatc tggactatac agtggaaatg
     7321 taatctttga tggcgattta ttaattgaaa agttgaatcc ttttacaaat aaaatagaaa
     7381 gtttaaaacc aataagcaaa gaagaaaata catactattt aaatcttaca aaaatagatt
     7441 taaagtcttt atttaataac tttgatgtat atactaaaag cttagtaaac acagataaag
     7501 acacagttat aaataactta ggagaaacgc attctaaact taatgaatat atatgggttc
     7561 aaagaaacaa aaagttccca ctagatataa ttattgtaga taataaaata gtaggtttta
     7621 tctgtttatc cagagaaact tgtactatat taattatgga tggatatgaa gagtatactg
     7681 tattaaaaga atgggaaaaa acccataaaa atgaagagat atattctata agatttggtg
     7741 gaaattatat gattgatatg aaagatggta taaaactttc tacagatgta tatctccctg
     7801 attttgtaga ttctaccaaa aaagcaccaa caattcttat gagaactcca tatggaaaag
     7861 aaaatgataa agaaatatat tataaatatg tacaaagagg atatgctgtt gtaatacaag
     7921 atgttagggg aagaaatgaa tcagaaggta agtgggaacc tatgattcat gagagggaag
     7981 atggagatag taccataaat tggatagtat cacaggaatg gtcaagtgga atagttggaa
     8041 tgcttggagc atcatatctt ggttatgtcc aatgggcagc tgcatcttca ggcaataagc
     8101 atcttaaagc tttagttagt atagtaacat caggaagccc ttttattgat ataccaagaa
     8161 aaggaggagc ttttgtatct ggtatgcttg catgggcatt tatggtatca agaaataaag
     8221 ttgatagaag taaaatggta agagatgatt gggatgatgt tttaaacata cgaccaatag
     8281 aaaatatacc tgttgaagct cttggatata gaattgaatt tctggaagaa tggttaaaga
     8341 gagtagaaaa agatgagtat tgggatttga tggattggca tttacaaaaa gataaaataa
     8401 atgtacctgc attggttgta tctgggtggt atgatgataa tagtatggga actacagagg
     8461 ccttagatgt tataaaggac tatgaaaaag gaaagagaaa ggctattcta ggtccatgga
     8521 tgcataattc aaatacttta agagatataa atggtatttc tttaggaaat agttcattaa
     8581 gatatgattt ggattataat tatctattat ggtttgataa atacttaaaa ggtatagaaa
     8641 ataacataga tacgactgct ccagtagagt attactctgt aggttttaat aagtggaaga
     8701 cagaagaaaa ttggccaata ataaataaga tagataaaag tatgtatcta ataagtgatg
     8761 gaaatgctaa cacatcttta ggaaatggta gattagtatt tgataatgat ttagaagaaa
     8821 agtatgatag ttatatatat aatccaaaag acccatcagt acaactaatt gatatgtcag
     8881 agaatgaagt tggagttcca aataactaca aagatttaga aaaaagaagt gatatgttgt
     8941 gttatacatc agatgctttt agtgaagaat ttactgtaac aggtgatata aagttagagt
     9001 tttttgcatc aagttctgcc aaagatactg actgggttat aaaaattatg gacgtggatt
     9061 tagatggaaa ttcaattaaa ttagctgatg gtatacttag tgctagattt agaaatagtt
     9121 tttataagtc agagtttatg gaagaaggag agatttataa gtttacgatt ataacttcaa
     9181 aaatatcaaa cacgtttaaa gttggtcata aaattagact agatataacg tctagtgcta
     9241 aaaactttat tttccaaaat agcaacacga aagagggata taatagtatt gaatatatag
     9301 aagcaaagaa tacgatttac catggaggta aatatccatc taaattgata ttgccaatag
     9361 aaaataagta aatttatata gattaaatag tgaggaggtg tctcaaaatg aattttttag
     9421 ttcgtgagac actttctttt atacgaaatc aaatatattt tcatcatttc aacaatgaaa
     9481 aagaggctta tctctctgtt ttgagatagc ctcctataag ctaaattgat tattttgtag
     9541 ctgcaacaac gctcatttca actaacaaat gctctctagc aagtctagct tcaacacaag
     9601 ctctagcagg ttcaaatcct ttttcaaccc aagcatccca aacagagttc atagcttcaa
     9661 agtctttcat atctcttaaa taaatagtaa cagatagcaa atgttgctta tctgaaccat
     9721 atttgtttaa taaatcttct attttagcta aaacttcagt tgtttgttca attactcctc
     9781 cttcagcatg agtttgtcca cataaataaa ctgtattgtt atgaactaca gctctactca
     9841 ttcttccagt tccttcatat ctctttattt ccatatttta aatacctcca tagttaagaa
     9901 atctattaaa aatctaaata ataaattaaa aaaagtctat actatataaa attatattat
     9961 atattctgat aataatccat aaaaacatat aactttttag ctataattat tattgttatt
    10021 aattgaaaag acttattagt tacttgacat agtttacact tttaattata gtataaattg
    10081 gaatatttag ataattatta tttaatgtat aagctaagtt tttaattaat atggctttat
    10141 acatttatat agaaaagagg tacgtatgag tagcacaatc aaatcaaatc gaataaaagg
    10201 tataatgttt ataatagcat cagcgcttgg ctttgcaatg atgtctgcat tcgtaaagct
    10261 agctggagat ttaccaagtt ttcaaaaagt gttctttaga aatctggtat cagctataat
    10321 agctttttgg cttatcataa aacataaagg aagtctaact ggtaaaaaag aaaatttaaa
    10381 agtactttta tataggtcaa tatttggaac tctaggagtt atatttaact actatgcaat
    10441 tgacaagttg atgctttcag atgctaatat gctaaataaa atgagtccct tccttgtagt
    10501 aatattttgt gcaattgctc taaaagaaaa aataaatgta aagcagatag gagctattat
    10561 aattgcattt ataggagctt tattcataat aaaaccaaca tttagtgtag aagtaattcc
    10621 atatcttggg ggaattgcag gagctgtatt tgcagcaatg gcatatactt gtttaagagt
    10681 tcttggagat agagaagatt actatacaat tgtatttttc ttttcagttt tttcattagt
    10741 tactgtaggg ccaattgctt ttgcagtata tgaaccaatg acccttatgc aacttatata
    10801 tctattacta ggtggagtat ttgcaagtct aggtcaattt ggaataactt tagcatataa
    10861 atatgcacct gctaaggaaa tctctatatt tgactatagt aatataatct tttcagcaat
    10921 tttaagtata ttcttattca atgtctatcc agatatgtta agtgttgttg gatatttaat
    10981 agtattttcg gcagcatttt atatgttttt atacaataag aagttagata aattagacaa
    11041 agaaaagaag aagtaaacta gataaaagtg tttttataat ttgtattgaa tatttaagtc
    11101 tttgtattaa aattaaagaa aatttaatta aaaatgtttg gtatattaac ttataacagc
    11161 agttaaaatt gaaaaattat aactgctgtt tcttttatta aaaatatcca aatgtctgta
    11221 aaaatagact ataatagagt agtataaata atataaaggg gataaattaa agtatggaaa
    11281 aagtgaaagt tgctgtgatt ggagcaggta atagaggaac gtatgcatat gctccatata
    11341 tttatgaaaa ttctgatata tgtgaaatag ttgctgtagc agaacctaaa aaaggaagac
    11401 gagaactatt tacacaaaaa tataacctag atagtaaaaa tgtatttgaa actttagaag
    11461 acttttttaa acatgacaag atggcagatg cagtaataat agctacaaat gatgatagac
    11521 attatgatgt agcaaaacta gctcttgaaa aaggttatca tgtactatta gaaaagccaa
    11581 tgtctaatag tttagatggt ttggtacata tagatgactt gtgtgataag tataaagata
    11641 agatttttat gatttgccat gtactgagat attctccatt ttataataaa ttaaaagaaa
    11701 ttgtagaaag caaaaaatta ggagagctag ttagtataca atataatgaa aatataggat
    11761 attggcattt tgcacatagc tttactagag ggaattggag aaatagcaat gaaaccagcc
    11821 ctttaatttt agcaaagagt tgtcatgaca tggatatttt gttatattta gtaggaagta
    11881 gatgcaaaaa aatatctgca tttggaagct taaagcatct taataaccaa aatgctagtg
    11941 gagagatggc acaaaattgt cttcagtgtt tagttgaaaa aaaatgtcca tattcagcaa
    12001 agagaattta tttagaaaaa gatagaagta taaatagagc tgttcacata aatccaactg
    12061 aagagaatct attaaatata cttaagacaa gtccatatgg aagatgtgtt tacagatgtg
    12121 ataataatgt tgtagacaat atggtaaata tattacagtt tgaaaatgga gtgacggcta
    12181 cttttaattt atgtgcattt actaaagaaa atggaagaac tataaagtta atgtttagtc
    12241 atggtgaagt tggtggggat ttaaataaaa atgagataag aattaaaaaa tttggaaaaa
    12301 acgaagaaat agttatgaat ccatcaaatc aacagaatat ggtagaggaa tatgatagaa
    12361 atcttatagc tgaatttatt aaattagttt caaataaaga actagaaaag ggtagagtag
    12421 cagcaaaaga agctatacaa agtcatgtaa tggcatttgc agcagaatat tctagggtca
    12481 gtgatgaagt agtgtatata gaagaatttt ttgatagtgc taagcaaatg actaaggaga
    12541 tagaagaaac tatattttaa ttaataaaaa tttattaaat aaaataaaga tagattaaat
    12601 aatagaaata cgaaaatatg tgttctatta aggaatctat cttttttatt aaggtttttt
    12661 cttatcccta tcaaaaaaat ccatacattc tgataaaaga taagaaagta aagcgaataa
    12721 gatttctgta cttaaaggaa tcgttactga atccataaag gtatcaatta tgttcatagc
    12781 tagaaaagtt agtataaaat caattataaa ttcgcaaagt ttatatattg agtatggtaa
    12841 tttgtttaca tatctgaata tatctaaaat agtagtgcat agaaaatcaa ttggagtggt
    12901 tatacaaaaa tatattaaaa agaaaattaa tacagctcca agagatttat attcaaatcc
    12961 taagaatgat aagaagccaa ttcctataac cgtgaaaata caaaaagtta taccaaaaaa
    13021 aacagcaaaa aataaaaata cagaaataaa ttcgcctaaa ggaatccttc ttttattact
    13081 tatcttaatc aactccttaa tttatgatat gaaatttata taaataataa cattcttaaa
    13141 aattcataat agtcctctgt ttcaaatagt actgtttgag aatctatttt tttcatattt
    13201 ggataaaaag aggccttaaa agctttataa tgttctctaa attttatttc tatcttaaat
    13261 ttatctggca attttattaa gtgcctagat aaatctccag ataaagcttt tttgacacct
    13321 ttttcaattt tttttagtgc aaggtttgga tggattgaaa ttgaaccatt tccaattcct
    13381 tttgacacag gaacagtgac tatatttgga tttagttttt ttgcactctc acaaagcatt
    13441 tcatctccac ttaaaaatgc tacaggaaca ttataatatg cagcaatata agcatttata
    13501 gtaaattcag atgctatttc atcatttatt ttaaaatagt cataatcaat atccatagta
    13561 tgagaaagtg gattaccatt acaagtagct ccagagtgat agccggtgaa tatacatgca
    13621 tcaaagcttt catcaattcc agccatcata actagtgggt ctttggtcca tcctctaagt
    13681 attttgacgt tttcaggtaa aatatttgga ttaatatttc taccactgtc atgtgcatct
    13741 ttaacaagta tttcatgtac accagattga gtagctccat cacatgctgc tgttatctct
    13801 ttactcattt gtgttctata gtgttgtgaa aattcacatt caagttttgt ttcatcccaa
    13861 tttacaatac cacaagtacc ttctatatca gcacttaaat acaatttcat aatatatacc
    13921 cccttatgtt tattattgat gataataaca gataacagaa aatacataat caataaaggg
    13981 cataaaaata ttatgtctta ctattaatta ttattaaatc tgtaactgaa aaataactaa
    14041 attggtatta aaaaatataa cactatgtgt catttattta tatagttttt tatatctgat
    14101 gtttatataa aattgtaaca tataagtggt tacatttact agtttaacat agttaaaata
    14161 gttaggtatt atttttaagt taaattgatt attataaaat cgtagaataa aagaaaaatt
    14221 taatttatta aaatgcatga aatgtttttt cctcaacata atgctaaatt aaactcaaaa
    14281 agaaataata gaaaaatctt atgtagctta gaaagaatgt gaatgattta agggaaaaat
    14341 agaaatttat tatataatat taggctgtat tatgtaatac agcctaatat tatatgtata
    14401 tgtgacataa aatcttggta agtgtaaata aaataaatgt ggaaaacaaa ttaatgaaat
    14461 caagaaaaaa gtaatttaaa attcaatttc atcttcccaa ttaaattctc ctgatttttc
    14521 tacactctta ggccagtgac tacaccattt aggaacggac ggattttcaa ccctatcaac
    14581 gcttggagta tatggcttta agtctataac aggactatta tcattagcat caatatatgg
    14641 tatttttatt ataccatttt catggtcgat attgataatt tgtacagcag tcaatgctat
    14701 tggattgggt cttataggtg accttgttgc aaatattcct ataacgtttg gtccactttt
    14761 ataaggtttt ggggtttcta aaatacttct tagttcagga ttatcaaacc catcagccca
    14821 ccaaaataca tttaaatgac tgaaatcgtc gagttcattt aatgcaggta catacttttt
    14881 gtctaattta ataaagacct cttcgccttg aatacatatt ttacctatag gatttattaa
    14941 tatttctttc ataatattac ctccaactat ttaatttatc atttgtaatt ttagggtata
    15001 acatgacatc atgtgagggt caatataaaa taataaataa aaatattaat agttagaatg
    15061 ataatacatt ttttacatgg agcatgacac cagtaataag gcaatcttcg tcaatatcaa
    15121 aagtagaggt atgcaaaggg gaagtaatgt ttttttcacg atttgcacaa ccaaggtggt
    15181 aaaaagctcc ttcacagtgt cttagaaaat acgaaaagtc ttcagcccca agagtgggcg
    15241 agtctttaag gataatattt tcttctccaa acaattctac agcattttgc ttgacgtaat
    15301 ctactagatg gtttgaattg ataagagctg gataaccttc ctcaatttca acagaaatac
    15361 ttcctccaaa tgttttacaa gtaaaatcgc aaatttctct aattcgtttt tttgaaaact
    15421 ctctagtttc aggagtcaag gttctcaaag taccccttat aaccactttg tcacaaacta
    15481 tattttcctt gattccacct tcaatctttc ctagagaaat aaccacagaa tcagttggat
    15541 ttgtgtttcg actaacgatt gtctgtagag ctgtaataat atgagcagat ataacaatag
    15601 catcaatacc ttgatgtgga taagctccat gacattttga accatgtaca gttaattgaa
    15661 gtgtatcggt ggaagcattt agagtgttgt attttaattc aattagattt ttgttgatat
    15721 tgggatttac atggagtcca aatatatatt ttacatttgg gttttcaaga catccatctt
    15781 ctatcataag ttgagcccca ccaatagttt cctcggcagg ttgaaaaaag aattttacat
    15841 ttacatttaa gtaattcttt aatttaaata gtatattaca agtacccagt aaaatagcag
    15901 tgtgtgcatc atgaccacat gcatgcattt taccaatatt aatagattta taagaataat
    15961 taagttcttc tgttataggt aaggcatcta tatcagctct tatagcaact gtttcaaagt
    16021 ttggactcac gttaatgtat gcagttatcc ctgtatgatt cttatattca atataattta
    16081 tacctatttc atttaagtat ttaataattt ttttcttagt ttgaaattct tctaatccaa
    16141 gttctggagt cttatgtaaa tctcttctta tatttataat ccaatcattt atctcatgac
    16201 aaagagttct tatattaagc ataaaacaca tcctaactat attaaaaatt tatttttctt
    16261 cattaattat attattttat gtaaatcatt attatgtatc aagtacaaat aaaatatctt
    16321 ataattatat tagagatatt tagtatggag aagaatatat cattttataa gaagaggtgt
    16381 ttaaatgtta ggaataattg cgccatctgg gccattaaga aatacaagtt tggaggaaat
    16441 aaagtttaat ttagaaagtt atggttacga agttaagttt tctgaaagtt gtagtttaaa
    16501 ttataaaggt tatttagctg gtaatgatga tattagagca agggatatag aagatatgtt
    16561 tttagataaa gatgtagata taattatgtg cttaagagga ggttatggaa caacaagaat
    16621 attggataaa ataaattatg atataataaa gcaaaatcca aaaccattta taggtttttc
    16681 tgatataaca ggtttaaatt tagcttttta taaaaattgt ggacttttac catatcatgg
    16741 aataatggca gcaaatgtag gtaaatggga taaatttacc tataaatcat tggtaaatgc
    16801 tttagaattt aaagatgact tatatttaga aaatccaaaa gaagaaaaga tttatactgt
    16861 atgtgaaggt aaagcagagg gaataataat gggaggaaat ttatccttaa taattgccac
    16921 aatgggaaca aaatatgaaa tagatgcaaa agataaaatt ttgtttattg aggaaattgg
    16981 agaacctcaa tataaactgg atagaatgtt aacacaacta tattcttcag gaaaacttga
    17041 ggaatgtaat ggtataattt ttggagattt taaggattgt attgaagaaa atgatttgat
    17101 ggaattacta atagaatttg caaataaggt caataaacct tctatatata atcttcagtc
    17161 aggacattgt atcccaatga taactattcc tttgggcaga atgtgcgaat tagatgctac
    17221 agaaaaaatc gttaaattaa aaaaatagtg tgtatataaa tataatagaa atatggtata
    17281 atcttataca agtaaaaatt tagctaggag gatgaaaaat gtctgatgat agaataatag
    17341 actttaacga actaaagaac aaagttaaag attctgatgt agataaattt gagcaataca
    17401 tatataactt atatttttct gttatggatg gaaagatgtc catggctgag ttttcaagaa
    17461 aaatattcga ttatatgaga gataacaaca tatctcaaga aaaatttatg aaaatacaaa
    17521 agcaatttat ggagagatat ggtatggaca ctgaagaagt agaaaaacaa cttagaaact
    17581 ttggaatcga cccttctaca gcaggtttta tgagtaataa tacttccagc aaagtatcta
    17641 cagaagattt agagtcattt aagaagagtg caggatttta tgagaaatat ggagaaaaaa
    17701 tccaaccaaa gagttgtata acaacattta taaaaaatga cttaaatgat attaatgtaa
    17761 taatcgacca agaaaagata atgctttgta gtgatagaaa aataaatctt atggattcag
    17821 aactaaatga gtttttatta gaatataaga atatgtttaa taaaaaaata aaggtagtaa
    17881 tgtgcgaaac tacgaataag tatgattatt aaaaaatgaa tttaaatact gaagtagtat
    17941 caattaattg tactacttaa aaataaaaat cgcaatttat tattccttat aaatttgtat
    18001 aaggaatatc taaactgcga ttttttatat tgtgaagatt agtcataata gatagtgttt
    18061 taaattgtaa tcttctcact aaaactgtgt ttttctatta gaaattttat taaaaataaa
    18121 tatctgataa ttgtattata tgggatgtaa agtattagag gcttaacgaa tctcttttaa
    18181 tacattaact ccattttgaa tcatactgtt gaaaaatact aagtttaaaa tctcagcagg
    18241 gtgaatgtta ggaatatcat aagtatctac taaattcata ggtacaacta tatttttttc
    18301 aatattatgt tgattaaaat atgattttag agttattgca aattgatata tgcatatatc
    18361 tgtgcaatcg ccaactataa ttatattatc tatattatca aaatccaaat tttctaatgc
    18421 aaaaaatcca ttagtagaat ttttaggaag aatttgtagg ttttctatcg acttaagttc
    18481 atcaaccaat tcactttcta catcattttc caaacagtga acaggataac tcaaaagttc
    18541 tattgattta ggagtatgag aatcagtaaa tgctattact ctatttaatt tgtttgatat
    18601 tttttttgta aacatttcaa ttggttttat taaagactct actctaggag agtataatgc
    18661 accttgtctt gcaaagccat tattaacatc aattataaat aaagcagttt tactcaaatc
    18721 ataattttct attttttcta ttggtagatt atctaaatta ctctttaaag cttcaagttc
    18781 atttaataag ttatccatag ttatacctcc aagataaatt taataaaatt gtacatatta
    18841 attatataaa agtcaaggag tacaaactat tgtataatta gctgtattgt tttgagtgta
    18901 aaagatttga agcaatatct ggatagttag taaaaatacc atctacattg agttttagac
    18961 agtgtagtat atcatcctct tcatttacag tatatacatt gacaccaagt agatttttgt
    19021 gacaaagttc aattagagat tcatttacta aaactagagg tggatgaagg taactattac
    19081 ccagaaattt agcaaaattg actacattgt ttataggtaa ataacacaat aatcctgttt
    19141 ttacactagg gtctaattta tgaaagtttt ttaagctatt gctgttgaaa ctagatataa
    19201 gaactctatt tttcatatta tactttaaaa taagctctaa aacgtctttt tctatattgg
    19261 gatagtttat tttgtcagtt tttagttcta tatttaaaat aaggtctgta ggtgaaatta
    19321 gaattaatac ttcttctaaa gttggaattt tacattcttt attgttttga tatccttcaa
    19381 aagatgagtt tagagttttt aattttctta aagtaaagtc ttttacaaaa ccatgtccat
    19441 taaaagttct atcaaccttt tcatcatgaa taactacaat ttttccatcc ttagttctgt
    19501 ggacatcaag ttctattcca tatatatcca tatacaaaca ttttttaaaa gctagaatag
    19561 tattttctgg atactttcca ctaaaccccc tatgagcata aatattcatt tatatggttc
    19621 cctcctataa gtataactta tcttataaaa atttaattaa tataatatat catattccaa
    19681 aaggtattgt tatatattag ttaaataact atttgtaatt tatatattat ataaatgtag
    19741 aaaaataaaa tcatatatgt gttataatat attttaaaca ttgtaaatat tacgaattta
    19801 gtagtcataa taagtgtaat tttatgcaag ctcatgtttt tagagcttgc atatgtatta
    19861 ttaatatatt tatatattgt gaaaataaaa ttccaaaaga attttttcta tagacttagt
    19921 agtaaggggg aattttgata tgtatgaagt taatttagaa cgagctatga attattggat
    19981 ggatttgtta gatgattata cgaatatagc aaaaattcca aatgattttt tttataaaga
    20041 ttgttctgat gaagtagtag atgaggtttt taagatgaat attgagaaac atgaaaggtt
    20101 aattcaatat tctaaagtga acaatattag catagactct atcatagaaa ctatatatgg
    20161 ccttatactt caaaagtata tatatgaaga tgatgtagtt tttggtaaaa caatgaatat
    20221 aattccaata agagtaagag ttagacaaag tgaaaaattt atagatgttg taaggaagct
    20281 aaaaaggcag tggagcaggt ctttagagta tggatactat ttgttatcag atatagagaa
    20341 taaaaatcat ctaaatacta agcttataaa cacaatattt atagctaaaa gttttgattt
    20401 atcatatgat tatgacatag aaaaaaaagg atatgattta tctgtaatag tatatactaa
    20461 gagggcactt aaaataaaaa taaagtataa accatctata tataaaagag aaactattag
    20521 aagaatttta aatcaatttg attatataat agagcaaata atagaagata atgatatttt
    20581 aatagaagat ttagaatttg tatgtaagga agaagaaaaa gtattattgg aagactttaa
    20641 taataatcaa tatgaagtac cctatgataa aagttatgtt gatttattta gggagcaagt
    20701 taaggtaaca cctagtaata tagctgctag ggatgaaaat agaaagttaa catataaaga
    20761 attggatgag gtaactgata aacttgcagg ttatttaaat catataggag tggaaagtga
    20821 agacatagta gctgtaatgt taccaagaga tattaacata attattacag caatagggat
    20881 tatgaaatca gggggagcat tttttccaat agacacgagt aatccagaag aaagattgaa
    20941 ttaccttctt gaagatagta atgcaaaagt agtgataact acagatgaac tcaaaagtaa
    21001 ggttgtaaat gagaatacca tagtactcga tataaatgat gaagaaatgt ttaagcttgg
    21061 atatgaatta actgaaaaaa taacatcatc aaattgtgca tatacgatat ctacttcagg
    21121 gtcaactggt aggccaaaga ccatagctat agaacataag agtttagtaa atatgtgtta
    21181 ttatagcgta aaatctataa gcgctacaga aaatgatata tgtggtatat acttaagttt
    21241 cagttttgat gctgtaatga aacaactatt cccttatttg cttgtaggtg catcaataga
    21301 tattatgcct gaagaagcta aatttgatga atatacagta aatgaatact gtgaggaaaa
    21361 tgacataact atactagcat tgccaacagc atttgcaagg ttatttatac aaaattgcaa
    21421 taataattca cttagagttg tacagactgg aggagaaaga ttaaaaggat atagaaagcg
    21481 taattatgaa ctatataatg agtatggacc aaccgaattt actgttgtat cgacttcttt
    21541 tcatgtggac agagaatatg gaaaaatacc aataggaaaa cctatattta atacttatgc
    21601 atatgtatta gataaaaaga acaaactatg tccaattgga gcaccaggag agctatgtct
    21661 atcaggcatt caaatatcaa gaggatattt aaataaaaaa gggctaacag agcaagtatt
    21721 tgtagagaat ccttacaaaa cttgtgaata taataagtta atgtatagaa ctggtgattt
    21781 agtaagatgg ttagatgatg gaaatttaga ttatattgga aggatggaca atcaagtaaa
    21841 aattgatgag tttaggatag agctatatga gattgaaaat ataataaata atattacaga
    21901 aattaaaagt gttgtttgta tatcgagaac aaatgatgac ggggatatgt atatatgtgc
    21961 atattatgta attgatgagg aagatagtgg taagataaat gaaagaacca ttagagaata
    22021 tttaaatgag catctacctc catatatgat tccaacaata ataatgagga tagataaaat
    22081 accagttaca ccaataggaa aagtaaataa aaaagcacta ccagagtgat tttttattat
    22141 gacaggtgtt gtaaagtgat tccatacatt tctttatgac acctattttt ctgaggaaat
    22201 caattgatgt aaaaaaaaga attttctgta tatatttact atggtgaaaa tttatttata
    22261 agaatgatta ttgtattttt tatacttttc cgtgttttaa agcatttttt ttacaaaatt
    22321 aattttacat taaaaatt
//
LOCUS       DAGQWR010000066        22337 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.066, whole genome shotgun sequence.
ACCESSION   DAGQWR010000066 DAGQWR010000000
VERSION     DAGQWR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.066"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            308..1369
                     /locus_tag="MIW95_002128"
     CDS             308..1369
                     /locus_tag="MIW95_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBY3151453.1"
                     /translation="MRDFLKNYKSSIILLACTIIGVIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKSTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKSLMVAQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     VGTAIAMMGDRGNTIRKFLSEGTEAILKVVSIVMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAAMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGSVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
     gene            1382..1579
                     /locus_tag="MIW95_002129"
     CDS             1382..1579
                     /locus_tag="MIW95_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBY3151454.1"
                     /translation="MLVVSIFGFPVEAIPLLTVITTITDIPNTILNTTGNTVSSMLVS
                     RLVEGKDWLIDKTAITTKKIS"
     gene            complement(1743..2471)
                     /locus_tag="MIW95_002130"
     CDS             complement(1743..2471)
                     /locus_tag="MIW95_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBY3151455.1"
                     /translation="MKTNYRLHMQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEM
                     LECLGVVDKTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLF
                     GEVFACTRLHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKN
                     ILLHNKIELLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRE
                     LGKMRDEGLLSFNKNKFEIFYFQK"
     gene            2612..3337
                     /locus_tag="MIW95_002131"
     CDS             2612..3337
                     /locus_tag="MIW95_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY3151456.1"
                     /translation="MMRKIIKIDEGKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            3397..4989
                     /gene="hcp"
                     /locus_tag="MIW95_002132"
                     /gene_synonym="priS"
     CDS             3397..4989
                     /gene="hcp"
                     /locus_tag="MIW95_002132"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBY3151457.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKLG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            5353..6258
                     /locus_tag="MIW95_002133"
     CDS             5353..6258
                     /locus_tag="MIW95_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBY3151458.1"
                     /translation="MKSSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            6382..7335
                     /gene="msrA"
                     /locus_tag="MIW95_002134"
     CDS             6382..7335
                     /gene="msrA"
                     /locus_tag="MIW95_002134"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBY3151459.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRIEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEVLGYGEYLKLFE"
     gene            7488..8618
                     /locus_tag="MIW95_002135"
     CDS             7488..8618
                     /locus_tag="MIW95_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3151460.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDINISDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(8735..9703)
                     /locus_tag="MIW95_002136"
     CDS             complement(8735..9703)
                     /locus_tag="MIW95_002136"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBY3151461.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTKL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPNTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLRECIALSKTL"
     gene            10038..10226
                     /locus_tag="MIW95_002137"
     CDS             10038..10226
                     /locus_tag="MIW95_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151462.1"
                     /translation="MNYHTKEELMEVLRVASSRIINCEKVQKKFSEETSHHTRFKNII
                     EAMYISKSLIMDEISKRD"
     gene            10486..11685
                     /locus_tag="MIW95_002138"
     CDS             10486..11685
                     /locus_tag="MIW95_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBY3151463.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEENKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            11737..12240
                     /locus_tag="MIW95_002139"
     CDS             11737..12240
                     /locus_tag="MIW95_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3151464.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            12347..12664
                     /locus_tag="MIW95_002140"
     CDS             12347..12664
                     /locus_tag="MIW95_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151465.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            12822..13781
                     /locus_tag="MIW95_002141"
     CDS             12822..13781
                     /locus_tag="MIW95_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBY3151466.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDIKRFKAII
                     ATSLVLGVLGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            13896..14681
                     /locus_tag="MIW95_002142"
     CDS             13896..14681
                     /locus_tag="MIW95_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBY3151467.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDGLEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            14973..16292
                     /locus_tag="MIW95_002143"
     CDS             14973..16292
                     /locus_tag="MIW95_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY3151468.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMSIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(16367..16795)
                     /locus_tag="MIW95_002144"
     CDS             complement(16367..16795)
                     /locus_tag="MIW95_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151469.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(16886..18262)
                     /gene="hydG"
                     /locus_tag="MIW95_002145"
     CDS             complement(16886..18262)
                     /gene="hydG"
                     /locus_tag="MIW95_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBY3151470.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(18263..19348)
                     /gene="hydE"
                     /locus_tag="MIW95_002146"
     CDS             complement(18263..19348)
                     /gene="hydE"
                     /locus_tag="MIW95_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBY3151471.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSPDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNKDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(19384..20592)
                     /gene="hydF"
                     /locus_tag="MIW95_002147"
     CDS             complement(19384..20592)
                     /gene="hydF"
                     /locus_tag="MIW95_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBY3151472.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVNKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(20607..20858)
                     /locus_tag="MIW95_002148"
     CDS             complement(20607..20858)
                     /locus_tag="MIW95_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBY3151473.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(21239..22279)
                     /locus_tag="MIW95_002149"
     CDS             complement(21239..22279)
                     /locus_tag="MIW95_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBY3151474.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         8363 a         2670 c         3554 g         7750 t
ORIGIN      
        1 aattattaaa tttgaaaata attaagcaat attatatata tttttttata ttgtttgttt
       61 tattaaaatt aatctagaaa aaaagtttta aaagcagaat tttattaata tttatatata
      121 gatatgaact attaataaaa tattaaagat acaaaaaaac tatagaaatt gacatatatt
      181 ccagtatttg ctaaagtagc tgttggggaa aattacaaaa attattaatg cgcattagat
      241 aatttacttt aattttttta ggatttaggg taatttataa tataaaacat tagaaaggaa
      301 aatataaatg agggactttt taaaaaatta taagtcatca attatacttt tagcatgtac
      361 tataataggg gtgatagtag gattagtact tggtccaaaa gcttcaatac taaaaccatt
      421 tggagattta ttcataaatg ctctattaat gatagtagta ccattagttt ttcttagtgt
      481 ttcttcaggt gttgctagta taaaaaatat ggaacgtttt ggaaaaataa tgaagagtac
      541 agctatagta tttatgggaa caacacttgt aattgcattt ttaggtatta catctgcatt
      601 attatttaat ccgtctaaag gattagatgt atcaagtgta aaaagtttaa tggtggctca
      661 agatgcagaa actttagaat caaaaggaac gttattagac caagtagtat caagtattac
      721 agttaaaaat tttggagatt tattttccag tagcaattta ctacaattag ttatatttgc
      781 agttttagtt ggaacagcaa tagctatgat gggagatagg ggaaatacaa taagaaagtt
      841 tttatcagaa ggaacggaag caatattaaa agtagtaagt atagtcatgc tttatgctcc
      901 tatagggcta ggctgttata cagctacaat aattggggaa cttggaagca aaataattca
      961 aggatatgta agggtgtttg taatttattc actaacagca gccatgtatt tcttattatt
     1021 ttacagtctg tatgcttttt tatctgcagg aaaacaaggg tttagacaat tctggagaaa
     1081 tgctataaaa ccaatagcaa cggcactagc tacttcttca agttcagcat gtataccatc
     1141 aaatttagaa gctagtaaaa atataggagt tcctgatgat atatcagaaa cagttatacc
     1201 tcttggaata aatatacata aagatgggtc agttttagca gtaataataa aagtggcatt
     1261 tttatcaggt attttaggca gtggaatggc aaatccagca acgataggtc aattatttat
     1321 aactgtattt gttgttggag tatttatggc tcctgtagca ggtggctgac ttattggtga
     1381 gatgctcgtg gttagcatct tcggttttcc agtggaggca attccattgt taacagttat
     1441 aactactata actgatattc ctaatactat attaaataca actggaaata cagtatcatc
     1501 aatgttagtt tcaagattag tagaaggaaa agattggctt atagataaaa cagcaattac
     1561 tacaaagaaa ataagctaat tgtacactta attaataaag ttaataggta tcttaatttt
     1621 aataaattag atatctatta actttttaag taaaaatata aatttaagat tcaaatatga
     1681 tatttataat ttgaattaac taatatagta tatattgaaa taaatatgtt taaatatcta
     1741 gtttattttt gaaagtaaaa gatttcaaat ttatttttat taaaagataa caagccttca
     1801 tctcgcatct tgccaagctc tcttgacata gaacttctgt caacacataa aaaatcagcc
     1861 atttcatttc ttgaaaaggg aattgtaaac tggtggctac ctgtttgttc tatctgtttt
     1921 gaaaaataca taagcaattt ctcgcgagtt gttttacgag aaagtaattc tattttattg
     1981 tgaagtaaaa tgtttttttc agctattaat tgtagcatat tttcaacaag cctattatga
     2041 aatacacatg cagatgaaca aataccagtt acacttttaa actttataaa taaaacttcg
     2101 caagaagatg ttgtggttac tgtaacatta cttttatgta gtctagtaca tgcgaatacc
     2161 tctccaaata aatctccagg caagagttca gctacaatat tcctattccc ttcgatatct
     2221 tcctttatta tatgagcact tcctttaagc ataattccaa tagaagtaac ttcacttcca
     2281 acagagaata ttatactatc ttttgggtaa gtcttatcta caacacctaa acattccaac
     2341 atttcttcaa gctcatcact tttaattcct tcaaatagag gaattttttt aagtaggtct
     2401 atatgctttt ctaaaaattt tttcaaataa tcacttcctt gttgcatatg caaccgataa
     2461 tttgttttca ttgtactata ctaagtttat aaaagcaagg ggatataaaa atcctactac
     2521 aagaaatttg aaataaagaa atataggtat taaaaataaa tatataagaa atcaatactt
     2581 taaattaaaa aaattttatg aggtgatata aatgatgaga aaaataatta aaattgatga
     2641 gggaaagtgt aatggttgtg gactatgtgt tgaagcttgt catgaagaag ctataggaat
     2701 ggtaaatgga aaagcaaaac ttttaagaga tgactattgt gatgggctgg gagattgtct
     2761 gccaacttgt ccaacaaatg cgatttcatt tgagtataga gaagcagcag aatatgatga
     2821 agctgcagta aaagcaaata tggaagcaaa gaaagcacaa aagaaaactt tagcatgtgg
     2881 atgtccaggt tctcagtcta agtcaataaa tagagaggtt tcaaatagta catctataag
     2941 caatgatata gtagagataa aaggttctca acttaatcaa tggccagtac aaataaaatt
     3001 agtaccaact aatgcatcat atttaaagaa tgcaagtctt ttaatagcag cagactgtac
     3061 agcgtatgca tatggaaatt ttcataataa atttatgaaa aataaggtta cattaatcgg
     3121 atgccctaaa cttgatgaag ttgactatgc tgaaaaactg acagagattt taaaagaaaa
     3181 tgatattaaa aatatagtag taacacgtat ggaagttcct tgctgtggtg gtatagtaaa
     3241 tgctgttaag acagctctac aaaatagtgg aaaaatgata ccatggcaaa tagttactat
     3301 atctgtagat ggaaaaattg tagatggaga aatttaatta agtgaatcat agaaaaatat
     3361 aataaatttt aataaataaa aagggaggaa taaaaaatgg aaaaaatgtt ttgttttcaa
     3421 tgtgaacaaa cagcaggagg taaaggttgt actataaatg gtgtttgtgg taaaaagggg
     3481 ggtacagcta acttgcaaga cgagcttaca ggagtgttga ttggtctttc aagagcaact
     3541 attggaaata agaatagacc aacaagtgaa acagataaaa ttatgataga ggggctattc
     3601 actactataa cgaatgtaag ttttgatgat gaagctataa aaagacaaat tgaaaaaact
     3661 gaggtagaaa aaagtaaact tgtacctaga tgttcagatt gttcaacaac ttgtaataga
     3721 aatgataact atgatatgaa ggatttatgg aatgataatg aggatatacg ttcattaaaa
     3781 tcattaatct tatttggatt aagaggcatg gctgcctatg catatcatgc aatggtactt
     3841 ggttatacag acaaagacgt aaatgaattt ttctatgaag gattaatagc agttggagga
     3901 gacttgtcta ttgacgaact tttaggtcta gttatgaaaa ctggagaaat aaacttaaaa
     3961 tgtatggaat tgcttgatag agcaaatact gaaacttatg gaacaccaga accgactcag
     4021 gtatctatga aaatcgagaa agggccattt attgtagtta caggacatga cctttatgat
     4081 ttacacaaat tattagaaca aacgaaagat aagggtataa atatatatac tcatggtgag
     4141 ttattaccag cacatgcata tccaaaacta aaagaatata aacatctaaa aggtaatttt
     4201 ggtacagcat ggcaaaacca acaaaaagaa tttgataata ttcctgctcc aatattattt
     4261 acaacaaact gtttaatgcc tgttaaagat agttatagag atagagtatt tactacagaa
     4321 gtagttgctt atccaggtat ggttcatatt gatgaagata aagatttttc tagtgtaata
     4381 gaaaaagctc ttgaacttgg aggatataga gaagatgttc atatgactgg tattaatgga
     4441 ggagatacat taactactgg ttttgcaaga ggtacagttt catctgtaca agataaagta
     4501 gtagatgctg taaagagtgg agctataaga cactttttat tggttggagg ttgtgatggt
     4561 gcaaaacttg gaagaaatta ttacactgaa tttgtaaaac aagctcctaa agatactgtt
     4621 atattgacac ttgcatgtgg aaaatataga tttaatgatt tagaccttgg ggatataggt
     4681 ggcttacctc gtttaatgga tatgggacaa tgtaatgatg cttatagtgc aattaaagtg
     4741 gcagtttcat tggcaaatgc ttttgaatgt ggagtaaatg atttgcctct tagcatggta
     4801 ctgtcatggt atgagcagaa agctgtttgt atacttctta cactacttca tttaggtatc
     4861 aaaaatatct atatagggcc ttctttgcca gcattcttat ctaagaatgt attagatata
     4921 ttggttgata aatttgcatt gacacctata tctactcctg aagaagatat gaagaagatt
     4981 ttaggataaa taaatatatc tatacttaaa gatatttata gttaaaatat ttttaagtat
     5041 gtcacaatta agataagggg ttaaacttaa aatttgatta aaaaatcaat tttgagacga
     5101 ccctttattt attttttgta ataattgaaa aataaacctg ctttcagtat attcttttaa
     5161 gaatttttgt attaaattta taatacttag atatataata tcttatatat tttattttaa
     5221 gtactaaagg tttgaatgta acttaaatat taaaagagga acattgtaat atgtaacttg
     5281 cataaaatat atatactata gtgaaattat tttgattaaa atataaggtg ttttatgtgg
     5341 gggtatatgt atatgaaaag ttctaatttt aatatattgg catttgatgg tggtggatta
     5401 aaaggggcat tgagtatatc catacttgag aggatagtaa aagagtatcc aaatctatta
     5461 aacaatataa atatgtttgg agggacatct acagggagtc taattgcact aggacttgcg
     5521 tatggtgtaa gccctaaaga gataaaagaa ctttactcaa ttgaaaattc taagtatatt
     5581 tttaataaaa gttatgcaga aatactcaga ccaaagtatg aaaacaaaaa cttaaaagaa
     5641 gttttattaa gtatatttcc agaggaacta gaattaaaag atttaaataa attagtaatg
     5701 ataccaagtt tttatatagg aaatgaggaa aatgcatgga aaccagtgtt ttataacaat
     5761 atgccaaatt cttttacaaa gactagcaaa gttgtagatg tagcaatggc aagtagtgct
     5821 gctccagtat tttttccaac atataatcgt catgttgatg gaggaataat agcaactgac
     5881 ccaagtttag cgtgtataat tcatgccatt gacagtggat ttaaacttaa gaatacaaga
     5941 cttttttcaa tagggactgg atatgtatat aatagtataa aggcagatac tactgaatgg
     6001 ggagctattg actggataat taataaagaa cccgatttac caattatatc tattacctta
     6061 gaaggaaatt ctcaaatgag tcagttattt tctcaaaaac ttcttggaga taattactat
     6121 agattaaatc ctaagatgga aaaagatgta gctatggatg attgtgatgc attagaatat
     6181 ctaatgtctt taggagaaaa ttgtgatata aaagatgctt ttggatggat aaagaaaaag
     6241 agcttttata gaaattagat tcaatttgca attgtatttt tgcacatgat ttcattgagt
     6301 aggtaaaaat aataaaatgc agattattaa gttacttctt agggaataaa taaagtataa
     6361 tttatttaag gagtgatgaa aatgataaaa cttgctactt ttgcaggtgg atgtttttgg
     6421 tgtatggtaa aacctttcga taagtatgat ggagttatta aagtagtttc tggatataca
     6481 ggtggtcatg ttgaaaaccc tacatatcaa gaggtatgtt caggaactac tggtcattta
     6541 gaagcaattc aaataacata tgatgatgat ataataagtt atgatgaact tctaaatatc
     6601 ttctggaaac agattaatcc tacagatgat ggtggacaat ttggtgatag gggaactcaa
     6661 tataagactg ctatatttta tcatgatgaa gaacaaaaaa atatagcttt agagtcaaga
     6721 gaacatctgg aaaatagtaa aatatttgat agtaaaatag tgactgatat aattccagca
     6781 actaaattct acgaagcaga agaaaatcat caggattatt ataaaaaaag accattaaac
     6841 tacaatatgt attataaagg gtctggaaga tataattttg taaaaagtca ttgggatgga
     6901 aacaatataa atagggaaga gttaaaaaag actttaactc ctatacaatt tgaagtcact
     6961 caaaatgata tgacagaagt accttttgaa aatgaatatt atgataataa caaagaggga
     7021 atttatgttg atattgtaag tggagaagtg ttattttctt caaaagataa atttgattct
     7081 ggatgtgggt ggccaagttt ttcaaagcca gttatagata aatctatcat tgagagaagt
     7141 gatttctcac aaggtatgta cagaattgaa gtaagaagtg ctaaaggaaa ttctcattta
     7201 ggtcatgtat ttgatgatgg tccaaaagaa cttggaggtt taagatattg tataaattct
     7261 gcttctctta aatttatacc aagagaaaat atggaagttt taggatatgg agaatattta
     7321 aaactttttg agtaattagg aatatttatt ataaacttat ctaaaaggtg aaaatataaa
     7381 aaaatatttt caccttttgt ttttttgtca ataatatgat aaattattaa ttgtaatctt
     7441 atttatacca atagattaag aatttaattt tgaaagagag aaaacaaatg aatttaaaag
     7501 actttaatgg aaagagatta aaatcagcta gaatttttag agcaaagacg atagaacagt
     7561 tgtctaaaga aactaaaata aataaaaaag atttaaaagc ttttgaggaa aataagtatg
     7621 taccaaatat agaaaataca ttaaaattat ctaatatatt aaattttcca aaagagtatt
     7681 tttataaaaa tgaaaatata aatatcgttg tagaggatag ccattttaac cctcaatcta
     7741 aactacctag agtagaagag atttcttata gagaaaaaat aattataata cataaaatat
     7801 attcatttat ggagaattat attaagtttc caaaagaaaa cttgccaaat agaaaagtaa
     7861 tgtcagatat taatataagt gatatagaat ctcttgcata taagactaga gagttttggg
     7921 atttaaatag tactccaata gtaaatatgg taagtttgtt agaatctaaa gggattataa
     7981 tatcaggtat gaatgtagat agaaaagggg caactatctt tactcaaaaa cagagaatta
     8041 gtaaagaatc taaatatcta atatctctgg gtaatgacaa aaagtcagca tcaattagaa
     8101 attatacttt ggcatgtgag cttggatata taatatcaag tgaattaaga ataccaagta
     8161 agcaattttc agaagatgag tatgcctgtg catttttatt gccaaaagag agttttttaa
     8221 aagatttgac tcatccagaa gatttagatt attatgtaga actaaagaaa aaatggattg
     8281 tacctatatc tgctatgatt tttagagcat ataatctaga aaagataaat tacaaaaaat
     8341 acaattatct tatgaatgaa atggataaaa aaggctggtt gatagaagaa ccactagaca
     8401 aaatgaaagg ttcaagccca acgtatttaa aaagagcagt tgaattattg atagaaaata
     8461 aaataatgag tgtaaatagt atagtatcaa gtttagagga atttggaata aatctttatc
     8521 cagaagactt agaattattg atgggattga aaaaaggatt gttatctcaa gaggtaaata
     8581 aaaaaagtaa agttataaaa tttgatggta aaaaataggt ttatatttta gtattaaaat
     8641 taaaagcagg tataaatgat ttaaacatta acttagttaa aagtcttttc aataagtcat
     8701 ttacctgctt ttagtgtaag aaagaatatt gaatctaaag tgtttttgaa agtgcaatac
     8761 attctcttaa aacagagcaa gatttattaa attcgttttg ctcatattca gtcatattga
     8821 tttcaataat ttcttcaata ccatcttttc ctataacaca tggaacacct gcaaatacat
     8881 ttctttcgtt atattgacct tctagtaatg tagaaactgg aagaactttt ttctcattgt
     8941 gtagtatagc ttttacaatt tctactaaag cagtacctat accaaattct gtacaatctt
     9001 taccatttat tatacttatc cctgtttttt tatttttttc aactattgat ggtaagtcta
     9061 aattgctata agtatttggg ttgtctttta tcatgtccaa gattggttta ccaccaattg
     9121 aaacatgaga ccaaggaacc atttgagaat ctccatgttc acccatagaa taagcttgaa
     9181 ttgattgttg agcgattcca gtttcttcag ataaaatcct tctaagtttt gttgaatcta
     9241 aagctgttcc agttccaatg actttatttt taggaaagcc agatttttcc caaatataat
     9301 ttgtaactac atcaacagga ttagatatga ctacaaatat accatcaaag ccagaatcaa
     9361 ctatgggttt tactatagat tttatcattc caatagttgg tcttagtaaa tctaatctag
     9421 ttgtgttttg tctacgtaaa ggcccttctg aagagtctgc aacacttatc actactatgt
     9481 cagaatcttt acagtcagaa aaagttcctt tttctatatg tactttatgt ggtagatatg
     9541 aaactgcatc agctaaatct aaagcttgag cttttgattt agattcatct atatcaatca
     9601 taaagagttc atcacaaact ccttgagtta ttaaactaaa tccacaatga gaaccaacat
     9661 gccctgaacc tacaatagat atttttcttg gctttattgc cattttaaat cactccccct
     9721 aaaataacga tacattgaaa attatagatg ttatcaaaag ctgtttttgg atattttaat
     9781 attttattat ttcagaaaat ttatttaata tgattttata ctaaaatttt taaaaaagca
     9841 atgttaatat actctctatt aatgaatgtt aaagtattat atgtatatta ataattatta
     9901 aaatatgtaa tgtcactgat agttagaata tttaagtgtt aaaagatata tataaataat
     9961 aatgatatca gttgataatg atattaaatt gaggtatact gtttctctaa attaaaaaac
    10021 aaaggagtaa atgtgttatg aattatcata caaaagaaga gttgatggaa gtactgagag
    10081 tagcatcttc aaggattatt aattgtgaaa aagtacagaa gaaattttct gaggagacat
    10141 ctcatcatac acgttttaag aatattatag aagcaatgta tatttcaaaa tcattaataa
    10201 tggatgaaat tagtaaaaga gattgaagct tattttgcaa tttaatatgt tttaatatac
    10261 attttattat ataagaaaat aagtaagcat acttgttata aaatattgaa ataatataaa
    10321 attataaatt tgttaacact aatatctata tgttaaaata tagtagaaga tgttagtaaa
    10381 taataggatg agttctattt taatacaatt aaattaagct tagcttgaaa tttgtattta
    10441 aaaagtaaag tagtctataa ttaagaaaat ggagatagaa ttataatgga aaagttatat
    10501 tatacaaatc agtatataag agattttaca gcagaaataa ttgaaataaa agaagtagaa
    10561 aataagtatc atgttttatt agataagaca gctttttttc ctggtggagg aggtcagttt
    10621 ggagacctgg gaaaaataga gaatgatgat gtaataaatg tgtatgaaga tggaaaaaat
    10681 atataccatg tattagataa aaaacctata aagatacata aggtaaagtg tagtattgat
    10741 tgggatagaa gagaagatgg aatgcatcaa cattttggac aacatgtatt atctggatgt
    10801 ttttttaaaa cttttaatgc aaatacgaca ggttttcatt taggaaatga gtatagtaca
    10861 gtagatatag aaggtaattt agaagagaac aaaattagag agatagagtt gtttgccaat
    10921 gaaattattc gccaaaatat acaagtagag acattacttc caacgaaaaa agaactaaaa
    10981 aagatttggt taagacgtga tttgccaaat actgatgaag agataagagt aataaaaata
    11041 ggtgatttag atacgaatgc atgttgtgga gtgcatccaa aatcaactgg tgaattgaga
    11101 atgataaaaa taaagaaatg ggaaaaacat aagtcaagta caagaataga gtttctagca
    11161 ggtaaaaggg cagtggatga agtattgaag agggatatat atttgactaa aatatgtaga
    11221 tatttaagct gtagtgagga agatgctata aatggtattg taaatctaaa tagtaaagtg
    11281 gaagaggctt taattaaaaa aagaaggctt gaagaagtag tatctacata tcaggtcaaa
    11341 gaaatgattg aaaaagcaaa taaggttggc aatatttcgg tcattaaaaa aatttatgat
    11401 gatgaagatt tgaaatatgt aaataagata gcaaataaaa ttgcagaata tgaaaatagt
    11461 attgcattat tagctgttaa atttaatgat aaaataaatt tagtttttgc atgttctaaa
    11521 aatttaaaag atattaatat gggagtttta ttaaaggatg caataagtct tatagatggc
    11581 aagggtggtg gaagtaaagt attagctcaa ggaggaggta agaataatgg aaatttagag
    11641 gctacacttg attatgcatt tatgaagata gaaaaaactc tataaatata ttatataaaa
    11701 aaccttagta gataaaatag atatggagga ataagtatgg agcaacaaaa ggactacata
    11761 gagttatatg aaaatgaaga actaatagag ataagagaag cacgtatgga tgatttggat
    11821 acaatagcaa aatttaatta taatttggct aaagaaactg aaggaaaaga acttgatatg
    11881 gatgttttaa ctaaaggtgt aaaagcattg ttattagatg agagaaaagg aaaatatcat
    11941 gtatatacag tatttgataa agttgttgca cagattatgt atacatatga gtggagtgat
    12001 tggagaaatg gaaatttcct ttggattcag agtgtatatg tagataagga atatagaaga
    12061 aaaggtatct ttaattattt atttaattat ataaaaaata tatgtgataa agatgaaaat
    12121 atagttggaa tgagattata tgtagaaaaa gaaaacataa atgcaaaagc aacatatgag
    12181 tctttaaata tgtatgagtg tgattataat atgtatgaat acgaagtaat acattcgtaa
    12241 tataaatttg taatactgtt atactgatgt tataaagtat tgattatatt gacgattata
    12301 tgaaataaac tggatattat aattagaatt tttagggggt ataaaaatgg gcgttttaaa
    12361 aggaatgttt tatgtatttt tatttttaga tttagtgtca atattctgtt tctttttcaa
    12421 taaaggaaaa atagcatcta ataaaattgt gtttaatgca ataggagtac ttacatttgt
    12481 actttgtttt atgcttttta gttattatcc taataataat cttattggaa aatttattgc
    12541 ttctctattt tttatatttg gagttgcagg agttatgttg aaagaaaaaa attttttata
    12601 tgctagactt cttttaactg tagttatagt tttttctaca ttaagattgt tcgttattca
    12661 ataaaatgta tttgtatgaa aatttattaa agtctacaca tttaagtgta ggcttttttg
    12721 attttttgtt tgaaaattaa ggaaatttta aggaaatttt aaaatcgtct taaatattta
    12781 ttgatatact taaaatatta atagcgaaag gagacttatc agtgatagaa agtaaaaagt
    12841 cagaaaaatt gttactgtca attatataca taatattttt ggtatatatt ttaatgatta
    12901 taagaataat tttatttaga gatgtaccaa tttacgctat tttcaaggga acgtttagga
    12961 atgtaaattt aattccattt tatacaatat atcaatttat agtagatagt aatcttgact
    13021 ttatgaaagc tactgtaaat attatagaaa atataggtat atttattcct atgggaattt
    13081 ttttacctat agtatgtaaa aatctaaata aaaaaactat aattatagta attatacttg
    13141 ttagcctagc atttgaatta acacaatata tttttgctct tggaagtagt gatatagatg
    13201 atgttatttt aaattcatta ggtggaataa ttggaattaa tatttacata aacatgaata
    13261 aattatttcc taatgatatt aaaagattta aggctataat agctactagt ttagttttag
    13321 gagttttagg attaggagtt atttctaaaa attatcataa cttactaact tttaaattta
    13381 gacctgatag aaaaatatca aaaatattaa ttgaagaaaa tagggaaata attaaagata
    13441 taaataaaga tacagtagat atagttggga catttgagag ctttaaaaaa ggaataataa
    13501 ctattaaagc aggttctaat aacaaagtaa agagaccaga gaatttaatt gattctgatg
    13561 gaaatataag aatttattta aatgaaaata caaaattagt atcttatata attaccaaag
    13621 aatcaaaaat ggatatagtt aaatatgagg aatttgatgt gaagaattta gatttattaa
    13681 ggagatatga tactattaat gtttggattg ataaagaaaa tcagccaaag gataaacatg
    13741 gtattatggc tagtaaatta ttaattggat tgtatgaata aaatattaaa caagaataaa
    13801 agaataacaa atataaaaaa cgaaaaaact atttctataa ttatttttac gttgtgcata
    13861 aaataagaac attagtgaag gagtagagat aaattatgga gataataaaa tatatcttgt
    13921 acttttttat atattcattt ttaggatgga cagtagaaag tataggatgt tctatagcaa
    13981 gcaaaagaat aatcaacaga ggttttttaa atggtccaat atgccctgtt tatggttttg
    14041 gagcagtaat tgtgatatca ctattaggaa gatttaataa tgtagtaata gtatttctat
    14101 taggaatgat tttaactacg atactagaat attttacagg atttatttta gaaaccttat
    14161 ttcatgctaa atggtgggat tattctgaca gaaaattcaa tataaagggt agagtatgtt
    14221 taaaaaatgc aatatacttt ggtgtaatgt ctgttttaat aataagattt atacatccat
    14281 ttataaaata ttttgtaagt ataattccat atagaatatt aatttcaatg gctataatta
    14341 caacactttg gactatactt gatttgattg ttactattat aacacttaaa aaattagata
    14401 taaagttaaa cttactagac gatataatta cagaccttaa tgatataaat gtgaaattgg
    14461 ataaatttga tagaggagaa atacaagctt tatttaaaac aataaataac gacggattag
    14521 aagttagaga aaaaataaac aaaataaata gtaagttgga ccgtatagag tctaatatta
    14581 ttttacaaaa aagagttata aaagcatttc cacatataaa acataaaaaa caacaagagc
    14641 aactagaaca ttttaaaaaa attattaatg agaaaaggta attatacaat ttaatgaaga
    14701 cgaatagaat aatggtattt ataagattat tttcagagga ctctataaaa atagaataga
    14761 gtcctttatt ttttgaaaaa taatgtttat ttgataaaaa taaaatatat tgtttctaaa
    14821 taacaaatat tttgaaaatt ataaaaacat atattgacaa tttgtgtcaa aaatactaac
    14881 attaagtata taaagaattt tatatgagaa tatctttata aatcgcaata attttagaac
    14941 ataaatttat ttaaagaggg gatgatttgc caatgagcga gataactagt caaatgataa
    15001 gtacagaaga aaaaaaatat gttgctaaaa ctcaaaagat accttattat ccagtagcat
    15061 ttaaatcagg tgatggtgca atgctatatg attatgaagg taatgaatat gtagattttt
    15121 tagcaagtgc aggctctgca aatgtaggtc atggtaataa ggaaatctca caagcagtta
    15181 aagaacaaat ggatgatata acacaatata cacttgcata tttccatagt gatcctcctg
    15241 taaaattagc tgagaagctt gtggaaatag cccctggaga taatgataaa aaagtactat
    15301 acagtgcaac tggttctgct tgtatagatg ctgctataaa attagcaaga gggtatactg
    15361 gaagaacaaa gataatatct atgtgtgaat cttatcatgg aagtacttat ggagctatat
    15421 ctatatctgc tttaagtaca aatatgagaa gaaaaatggg tccattgtta ccagaagttt
    15481 atcacttcca ttatcctgat aaaaatagaa cagctaaaga atgtttagat gagatagaat
    15541 atgcttttgc acactatcta ccagcagaag aggtagctgc aatatttata gagccaattg
    15601 caggagatgc aggaataata gtgcctccag tagagtgggt acaaggttta agtaaaatat
    15661 gtaaagaaaa tggaatatta cttgtaagtg atgaaataca acaaggtatg ggaagaacag
    15721 gtaagtggtt tggtatagaa aattttggtg ttgaagcaga ccttatagtt cttggaaaat
    15781 ctgttggagg tggattacct ttaggagcag ttgttggaag aacagaaata atgcaaagtc
    15841 ttgatgcacc agcacattta tttactttag ctggaaatac aacagtgtgt gtcgctgctt
    15901 taaaatctat agagataatt gaaaaagaaa acttacttca aaagagtata gaaatgggag
    15961 attatattaa agcaggattt gaaaaactaa aagaaaaata cgatataatt ggagaaataa
    16021 gaggaatagg gttatctata ggagttgata tagttaaagg aaaaggttct aatgaaaaac
    16081 atccagatgc tacagctaag atatgttata gatgtataca aactggatta ataatgatat
    16141 tcttaggaca atctacttta agagtacaac cacctctagt aataactaag gagcaagtag
    16201 ataaagcaat gagcatcata gattctgcaa tagatgatta tttaaatggt agaattggtg
    16261 atgaagtata tgaagtaact caaggttggt aaaataaata ttaaatgaaa aaaagagtta
    16321 tctcaatttt agaaagtaat ttctgaaatg agataactct tttaagttac ccacaaataa
    16381 gaaaattgtt tgattcatta aagactatat ttcggatgtc gtttttgtga gtatcagaga
    16441 cagtattacg agaataatta cctttattcg tactatctgt taatttagtt ataattaaga
    16501 tagctaaaag aataagaatt actgtgtata aagcaataac atatggtttt cttttatatt
    16561 tgtatttgtt attgaaaaaa gaatggccat aattattttt atttacttta gatgtatttg
    16621 tattcttttt cttgttacct ttctttttat catccttttt tctattttta gggtaaggaa
    16681 tgactttgtg tgtattatca agtttgatat ttttattttt ttgaatattg ttttccaggc
    16741 tagccttttt ttctccaaat tgaatagttt tattggtatt gttgttatct ttcaatgtta
    16801 atctccctcc cttaaacttt ccctttagat aatattacta ctaacctatg gaatatacaa
    16861 taaataatat ataagtaata aaattttata ggtataaatc tctttctcca cgctctaagg
    16921 cagtcatatt gttagatact atgtttctta tatcttctct ttctatctta cctatttctt
    16981 ctgcgataag ttttttaccc ataattttaa gttcttcatc tccataatcg attaaaaatt
    17041 catttaatgt agtaagagca tttggcgtac atacattatg gatttgtcga cttttagcta
    17101 agctcatgaa tctatcgcca gttctacctt tacgataaca agctgtacaa taactaggta
    17161 tatatccatc agtaatcagt tctttaagca cctcaacagg gcttctatgg tctccaactt
    17221 cgaattgttc tgtattgtca ccatcttcat atgctttata tcctccaaca cctgttagag
    17281 aacctgcact aatttgtgat acaccatatt ttagtaactc atttctcatt tcagcagttt
    17341 ctctagtgga cattatgatt ccagtaaaag gaacagccaa tctagttata gctactattt
    17401 ttttgaacat atcgtcagaa actaagtgag gaaaatcttc taagctcata ccttcagctt
    17461 ttttcaatct agggaaagat atagtatgga aacctacacc aaatttttcc tctaagtgtt
    17521 cgttgtgcat cataagtcct agaacttcaa atttagggtc agataaacca aataaaactc
    17581 ctgcaccaac atcatcaaca ccagcttcca tagctctatc aaaagctgtt aaatggtaat
    17641 aatagtcatt ttttatagat tgaccatgca tttttataaa tgttggtttg tgataagttt
    17701 cttggaataa tatataagtt cctattcctt tttcttttag taatttatac tcatctacac
    17761 ttgtagctgc tatgttaacg tttacccttc ttatatttcc gttttcatta tatgttgaat
    17821 aaattgcatc taagcaatct aagatgtaat ttatatcaca atttttaggg tcttcaccag
    17881 cttctagagc aagtctttta tgacccattt tttctaatat tttaacctct tcctttatct
    17941 cttccatggt aagttttttt cttttaaatt tgttgcattt actaaaaccg caataaacac
    18001 attcatttac acagtagttt gatacataaa gaggggcaaa taaaaccact ctattaccat
    18061 atatttcatt ttttatttgc ccagctatac taaataatct atctagttgt tttttatctt
    18121 ctacttcaag aagtgtagct atatctttat aactaagttt ttcccttcta tcagccttat
    18181 caagtacctt ttcaatatca tcagaagttg aattttttgc atcttctaat aaagaattaa
    18241 taagttcatg gtttatgaac atttacaaac gcctccattc aatattatca ccgcgagaat
    18301 agtcaacttc aagtcctaat gacttgatgt tttcttctaa actttggtga tattcacaag
    18361 cttcttgacc agtaaaggct ttgttatcgt aaagagaata ttgttttcta aattcttgag
    18421 gagaaaggtt tggcataatt acattgcaac cagctaaaag acctgcattt ctaccactag
    18481 ggtttataga tgccaaggct gttgtagcgg gtaataaaac ttttggaagt agaagtcttg
    18541 taattgaaag cataagtaat gtcttctcta aatctccatg tttataatcc ttaaaaattg
    18601 tatcatggtg tggaataaaa ggtcctattc caaccatatg aggatttaac tcttttaggt
    18661 ataataaatc acgaacaagg tctttgttgt tctgaaatgg tgattcaacc ataaatccag
    18721 cacctacctg ataaccaatt tctttaaggt tttttaggca ttcttttcta gttctaagct
    18781 caatgtttgg cggatgtaat tttttataat gactgtctgt agcagtttca tgtcttaaaa
    18841 gatatctatc tgcaccacat aaaaagtatt ttttatacga atcataactc ttttctccta
    18901 gtgacagagt tatggcacaa tcctcatatt tattttttat acttgaaaca atttcacata
    18961 ttttatcatc agtaaaatat gcatcttcac caccttgcaa aacgaatgtt ctgtatccta
    19021 ttttataacc tatatcacaa cattcaagta tttcatcagg tgaaagtctg tatcttatga
    19081 tatttttatt agaagattgt attccacaat agtagcaatt atttttacaa tagtttgtaa
    19141 gctctataag tcctctcaag tagactctat ttccataatg tttatttctc atgtcgctag
    19201 cttgtttata aagatagttt cttaaatttt gttcgaagtt atattcctca ttgtcgatat
    19261 tttctattaa atatagtagt tcatcaaaac ttaatgaatt aatgctgtaa agtttatcta
    19321 taaagtattt aatcttattt ttagtcatat tcaaatatgt aattattaag tatagttaaa
    19381 tttttaataa ttgaaaagtt caagtgatct ttccaaaata ccttttacat atgctattaa
    19441 gataccatag tttacaatag gtacattgaa agattttgcc ttttctattc tagataacat
    19501 tccagctcta ttcatcatac aagctccaca gtgaactaca agggcatatt tatttatatc
    19561 ttcagtgaag gatactccag aactaaattc aaaagtaata tttttgccag ttttctttct
    19621 aatcatatta ggaattttta ctgttccaat atcatcagtt tgtctatgat gggtacatcc
    19681 ttcagccatc aagattttat caccatcttt taaattttct aaagcataag ctccattaat
    19741 caattcttta aggtctccct tttgtctagc aaacaatata gaaaaagatg taagaggaat
    19801 atcttttggt gtatctttat ttacttgtgg aaatacttga gagtcagtaa taacaagttt
    19861 tggttttttg cctaaattag atagagtttc ttttaaacta tcttcttttg taactataga
    19921 aattgcacca ttatccaaaa tatctcttat aacttgctgt tgaggaagta taagtctacc
    19981 ttttggagct gctttatcta ttggaacaac taaaacaact aaatcatttg gttcaattaa
    20041 atctgatacc aatttgaact cagtgctatc cttaggcaaa acttttatta tttcattttt
    20101 taaattttca attccaattt tatctgttga tgagacagaa acaactggac atttaacttt
    20161 atttttagtt aagtttaaaa tttctgattg attcttaata gtatcaattt tgttgagaat
    20221 tagaatatga gggatattct tatcattaaa cttttcaatt agtgacaaat cttcttgtga
    20281 tataccaatt tggcaatcca ctactagtaa agctatatct gtcttttcta aaacatctag
    20341 tgatttacct attctaagtt caccaagttc accaacatca tcaagaccag cagtatctat
    20401 aagaacacaa ggtcctatag gaaggatttc cattggtcta aatactgggt ctgtagtagt
    20461 tccagcaata tctgagacta ttgcagcact ttgatttgtt atagcattta ttatgctaga
    20521 tttacctgca tttcttttac caaagagacc tatatgtact cttaccgatt gtggagtaga
    20581 atttagactc atttgtactc accaccttaa acttcttttt ttgatatact agtttttaca
    20641 gatacatgag gtatgttgcc caatttacca gtcaaactat taatagtatc catttcgcca
    20701 acaactgcta tacatacgat tgagacacct tcttcttcgc aaggaatgcc cattcttcct
    20761 ttaataatac ctttaaaacc tgatacaacc tcattgaatt taaattgaca ttcttttggc
    20821 tcttccaaaa tagcacttat aactgcaacc tttttcataa atatcctcct aactcagaaa
    20881 atatgtctta aacaaatcca aaatcaaaaa acttttcttt gtaaagaaaa gtttctacaa
    20941 aagaaaagtt attaattaca caagtataat gtgctatatt aaactatttc cttacagatt
    21001 agataactct tttctggtag ggttagcttg taaaaattta atttaacaac aagtaactga
    21061 agaaaatttt tatacaattt atataaatta taataaaaat taactaaaga aaattgaagt
    21121 tacttatata atagtagtaa attgaaatta agtctataga aaaaccaatt tttgtataaa
    21181 aataaaaatt aaagttaatt atttgttaag aattgacttt ggtaaaatat ataattaatt
    21241 attctttaag catataattt ataagtaatt ttagtgtatt ttcaatagct tctatatgtg
    21301 ttctttcata atggtgacta ttgtttgttc caggaccaac acatgctatt tgaacatctt
    21361 ctccccaacg aatagcttct gaagcatctg aaccatagaa agtaaataca tcgacagcgt
    21421 agtctaaatt attgtcttct gcaacatcga caagcttgtt tcttaaatta aaatcatata
    21481 atgataaatt atcttttgca actatagtta caccatgttc actagatgtt tgacttggtc
    21541 cagtaggtgc tatatctatt gctacaaatt cttttgtttt ttcaggtaaa catttagaaa
    21601 caccatgtcc aatttcttca taattactta tataaaagtt agtagtatgc ttaggcgtta
    21661 aattgttttc tttaaaatat ctacatattt caagaaccat tgctacagct gatttattat
    21721 ctatatatct agattttata aaaccagatt ctgtagcctc aaatctatgg tctaggcata
    21781 caaaatcccc aacacttatt cctaaatcta atacatcttg ctttgtaaat actttttcat
    21841 caagtctaat aatcattgtt tcttcatttc tttcagtggc agcttttgtt tgaccatata
    21901 tatgagtaga agcatactta aatacaacac ttcctctgat ttttttacct tttctagtta
    21961 taacagtgca gttttctccc tctatagctg cccaacatcc accaccaact ttatggtatt
    22021 taagagtacc atcagatgat atttctttta ctatagctcc aagtgtatcc atatgtgcag
    22081 atattgttat ttgatttgaa tcatcttcac caggaagagt agctataagt gcaccttttt
    22141 ttgtaagaat agtgtcaaga cctaaatttt caaaatcttt tttgatttct aaaactgcat
    22201 tttcagtata tccagctggg cttggaatgt ccaaatattt tttcattaaa tttatagtat
    22261 tagttaaatt taaattcata ttgtttcctc ctaaataaat atatttatac aaattataac
    22321 ataaatacaa atgttct
//
LOCUS       DAGQWR010000067        22288 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.067, whole genome shotgun sequence.
ACCESSION   DAGQWR010000067 DAGQWR010000000
VERSION     DAGQWR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.067"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..499)
                     /locus_tag="MIW95_002150"
     CDS             complement(<1..499)
                     /locus_tag="MIW95_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151475.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEV"
     gene            complement(601..1671)
                     /locus_tag="MIW95_002151"
     CDS             complement(601..1671)
                     /locus_tag="MIW95_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBY3151476.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDISVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSTSSYLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSNMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(1664..2722)
                     /locus_tag="MIW95_002152"
     CDS             complement(1664..2722)
                     /locus_tag="MIW95_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBY3151477.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNIKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSVTIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(2709..3041)
                     /locus_tag="MIW95_002153"
     CDS             complement(2709..3041)
                     /locus_tag="MIW95_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBY3151478.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            3240..4361
                     /locus_tag="MIW95_002154"
     CDS             3240..4361
                     /locus_tag="MIW95_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBY3151479.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(4489..6387)
                     /locus_tag="MIW95_002155"
     CDS             complement(4489..6387)
                     /locus_tag="MIW95_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBY3151480.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            6702..7478
                     /locus_tag="MIW95_002156"
     CDS             6702..7478
                     /locus_tag="MIW95_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBY3151481.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGVGDLKELYRNINYHKLPSSSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(7570..8322)
                     /gene="xth"
                     /locus_tag="MIW95_002157"
     CDS             complement(7570..8322)
                     /gene="xth"
                     /locus_tag="MIW95_002157"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBY3151482.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(8483..9433)
                     /gene="galT"
                     /locus_tag="MIW95_002158"
     CDS             complement(8483..9433)
                     /gene="galT"
                     /locus_tag="MIW95_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBY3151483.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            9818..11014
                     /locus_tag="MIW95_002159"
     CDS             9818..11014
                     /locus_tag="MIW95_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY3151484.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(11126..12472)
                     /locus_tag="MIW95_002160"
     CDS             complement(11126..12472)
                     /locus_tag="MIW95_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBY3151485.1"
                     /translation="MTMKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVV
                     EERQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKI
                     PLQFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALK
                     RVFPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDL
                     VPKIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKV
                     AKSNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAH
                     NKHQIAIVITENNGAINNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAY
                     EKLAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(12605..13357)
                     /locus_tag="MIW95_002161"
     CDS             complement(12605..13357)
                     /locus_tag="MIW95_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY3151486.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(13516..15093)
                     /locus_tag="MIW95_002162"
     CDS             complement(13516..15093)
                     /locus_tag="MIW95_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY3151487.1"
                     /translation="MKQKMKEFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     IPFLENNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGILAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYALVYYNVFKVLILRFNLQTPGREEITDINFDLEPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKENIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            complement(15506..15958)
                     /locus_tag="MIW95_002163"
     CDS             complement(15506..15958)
                     /locus_tag="MIW95_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY3151488.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(16269..17714)
                     /locus_tag="MIW95_002164"
     CDS             complement(16269..17714)
                     /locus_tag="MIW95_002164"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBY3151489.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINKRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(17858..18766)
                     /locus_tag="MIW95_002165"
     CDS             complement(17858..18766)
                     /locus_tag="MIW95_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBY3151490.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            19023..19847
                     /locus_tag="MIW95_002166"
     CDS             19023..19847
                     /locus_tag="MIW95_002166"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBY3151491.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILINEFIKLNF
                     SNTFINTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRVFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            19887..20417
                     /gene="lepB"
                     /locus_tag="MIW95_002167"
     CDS             19887..20417
                     /gene="lepB"
                     /locus_tag="MIW95_002167"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBY3151492.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSVVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEILG
                     KVMVRLLPLDNIGKVD"
     gene            complement(20499..22280)
                     /locus_tag="MIW95_002168"
     CDS             complement(20499..22280)
                     /locus_tag="MIW95_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBY3151493.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         7405 a         3536 c         2574 g         8773 t
ORIGIN      
        1 ctacttcttc aatccaaatg ctattatctt cttcttggat gacattttta ataatattat
       61 ttatagtata tattatattt tgttcattca aataatcatc tttccaagaa actctacttt
      121 ttttatctgg ttctatcaag tttattttaa caagattatc tttttcctta acttctactt
      181 ccatgtctaa accattaaaa tactcatttg ataatgtatc taagattaat cgcagtttat
      241 ccttaagtga actttcttca tcaatctgta agtactttac tattttctca tcctttgaaa
      301 ttatctctat aggtatttta gtagtttttt tagaattaat cattttacta gttgtttctt
      361 cttttgcatc ttcatccttc tttacaactg ctgattcttt agaatcaact agctcaactg
      421 cactgatgct ataatccata catgctgtac tcaataaagt agtagcaatt attaaaaaac
      481 ttactgctat tttattcata agtatccctc tcctaattct ctttcgatac ttattatacc
      541 atagtataga ttatattttt atacttttaa atcaatttat aaattattat agtaaattat
      601 ttacaattct aagctatttg aatttatttt tatagtatat tctttctcta aattcgacag
      661 atttttattc aaaagccctt ttatttccct cgtatttcta tacttgttgc tatgttcttc
      721 actatgttca taatcatatg aatcattgtc atattcttca tcattattaa attcttcaat
      781 tgcttctttt gactttatat cacaattaaa tttatactta tttactggta tatcaagtct
      841 taccttagaa tttggaactt ctatgtccag gaacttagca attggcatat tagagtacaa
      901 gtaaaattca ctttcataat ttgaatttct acctattttc tcttttatat ttacattttc
      961 aggtatatac tcttctatat tattgataaa tacatcttca ttctctgaag atatatccaa
     1021 tgactctgat tctatgttta catttttaac gcctaaaatt gattttgttg acaagtgaag
     1081 attgctatta gatattaagt ttaggttttc taatctattt actttgttct ctgttatagc
     1141 tctactaaaa cctccataag aagttttata tgttatagta tttgaagcta cattatcata
     1201 tacaaataag tatgaacttg tacttgatgc atttatgtct acattatttg gtacatatat
     1261 tataatttct ctataatcat gtgaaaacat actatttata gatgtattta ataattggtc
     1321 gaaattttta atctttttaa ttttattttc atattttctt tcacctttta caactaaagt
     1381 cttatctttt aaagttacat tgtatttgta aaactcatta tttcctcttg ttgttacacg
     1441 cacatcttca cttgaacttt tctttattgt aacgtttata ttgtcaacag atatatctaa
     1501 cttgtcaata tttacttttt cttgataaag tttattttct tttttaaatt cagcttcctt
     1561 ttctaaacca aagtttatta ggcttggcat aactaaaatc cctgaccatg ttgtccctat
     1621 tatacctatt accaataaaa ctgcagcaaa tatagcaagt tttttattca tgtttatctt
     1681 ctcctttctt attttctata tcttcaaggt ccttatatat cgcctcaaaa tttatactat
     1741 catcattata ttcatcagtt tcttttatgc tttcttcttc tctattgtag taatcattat
     1801 tatatgaatc ttcattaatg tctttattca aatcttcatc atttaaagta gctttttttc
     1861 ttttttccat cttttcattt gcctttatat aaatattttt tctctttatc caatgtatat
     1921 aattatttag taattgttta cctagtttta tagcaaataa taaaatttgc catgcaagta
     1981 tctcaagccc tacaaatgct atggttgcga aaatacaaaa catcataaca tctggaacca
     2041 ttgctataaa tttaatcaca aatggaatta atataacaaa agcaattaat gaacatacta
     2101 atgcaatagt aacagataca atagcacata caaacgcaaa tgctggaaca attagaaata
     2161 aagataacac tactaataat ccctttataa cttgtctaga taatccccct acaccttctt
     2221 ctatacttgg tgtaagttta tcatttatat tttcttttga tttattgtac ttttccttag
     2281 cttcatcttt caagatagag taatcctttt ttatatttga tttaaaccta ctaaatttac
     2341 catttaatat atctgtattc attatttttc catctatatt ttcttttgat ttactatact
     2401 tttcttttgc ttcatctttt aagatagagt aacctttttt catatctgat ttaaatttac
     2461 taaacttatc attcaaaaca tctgtattta ttattttttc ctcttcaata tttttttctt
     2521 tagtctgcaa gataagttct ttggctatag attttggtga accaagtgat gctattatct
     2581 ccatatcact ctttccctct ataagaccat ctacgaaata ctcttcataa tctctcaata
     2641 tatcattaac ttcgctctca gaaaaatctt tttttagata atcctttaaa atttcaagaa
     2701 actcagtttt attcaagttc attacctccc tcatcttcac ttattaaaat ctcaacagcc
     2761 tttacaaatt ctctccactc ttccatcaga ttggataaat tctcttttcc tagtgaagtt
     2821 attctatagt attttctagc aggaccttca ttagattcta gtatatatgt ttcgaaatac
     2881 aaatcttttg taagtcttct tagaatagga tatattgttc cttcatttac atctattact
     2941 ttagagatgt tatgtactat ctcatatcca tacatatctt tcttagatat gagagcaagt
     3001 acacaaattt ctaaaacacc ctttctaaat tgtgtgttca tatactccat cctttcttta
     3061 aatacacagt attgtgtttt acaaggtact atgttaaaac ttcttatatc ctcataataa
     3121 cacagtacta tgttctgcac aatagttatt tttaaatatt cttgttgatt aaatccttct
     3181 ataaatgtat aatactctaa tcagatatat tttatgaaag tggtgataaa ttggctaaaa
     3241 tgaaagatga taaaacaata ttaatcttaa ctgctcaatt tggtgctgga catataagtg
     3301 ctgcaaaagc agtcaaagaa tgtattattg aaaaatatag taactataat gttgttatac
     3361 aaaactttat aaatgcaagt attcccatga tgaataaacc tatggtaaaa ctttatgaaa
     3421 acaatacaaa atatactcct ggattatata attactatta ttactttaag aaatcgtttg
     3481 attcaagaca tgatttatct cataaattat atacacctaa actttctgag tatattgctg
     3541 atataaatcc agatttgatt atctctacat ttccactagc ggctgcttgt gtaaataact
     3601 ttaagataaa aaatcctgat ataaatatac ctacacttac agttataaca gatgttgtag
     3661 atagtatgga atgggttttt gaaaatacag atttgtattt tgttccttct cctgaaataa
     3721 aaaatagatt ttttcagaaa ggaataaatc cagactccat aaaggttact ggagttcctg
     3781 ttgataagag atttcaaatt gaaagtaaag aactatgttg tgataaatat agactattac
     3841 ttctaggtgg aggaagaggc ctatttgata tagatgagga ttttatgcat tggatagatg
     3901 aatttattga agaacatagt aattctatag aaattacaat agttactggt aaaaacaaaa
     3961 aattatatga caatctaact cataaaaaac ctctaaaaaa tattaaagta cttggatttg
     4021 taaacgacat gtataatcta ataagagaat gtgacctaat gctaactaag cctggaggag
     4081 caactatatt tgaagctatt caatcacaaa ctccagtact cgtgaaaatg ccaaaagttg
     4141 gacaagaaat tgaaaacgct aaatttataa tagataaggg tcttggcatg atttatagtg
     4201 atgacctaga ccttaaaaac atattttata aattagtatc aaatgaattt gattctataa
     4261 taaactttat gaaaaaaaat ttagaagaat ttaaaacagt tattcatcca gagaaaattg
     4321 ctgattatat atcagaatta atagataatc attacagtta aattgtacta ttaaaaaatt
     4381 tcaaacataa aattaaaggc aatcaagtat gcttttacac ttgagaattt actcatagtg
     4441 tttaataata cttgattgcc tttatcatat caaaatcaaa ttgttatatt agtatatatt
     4501 tttcttgtac tgcatataca gttgttgtat ttcttccata cttctcttta attctatttg
     4561 taaatctgaa gcagtctggt aatccttatt ttctatagct tctctcactc tagtgaatag
     4621 tgattctcta gttaaagtat ccttcataat attgaacttt aaatccgata ttgagttcca
     4681 aactacctca tcaagcgcat ccatattttc ttttgtgtgt atcttgacaa tacttcttat
     4741 tttttgaatc ccagcctcta ttattctagt ttttaatttt ttttgccact tatctatagc
     4801 tccaatctta aatgattcta ttatactatc agtaaataca tcaccttgtt ttaaagattt
     4861 taattttgaa gcatatattt ctaagttttt catattttca taaactgtag ccggtggtat
     4921 tccaaatctt gcatttcttt cttccaaact ataatgttca aatacatcat tttcatctct
     4981 atatgctcta tctttttcta aatagaaact ttcttcacct acatttttag ataactcttt
     5041 ttcaagttct tttgtactaa gaccactctt agctgctgct tttataccat ccaacattgt
     5101 ttgataacat cctgctatta ctaaatacgt gttagataga gggcttggtg accttaactc
     5161 aaatcttaca gtctttggat ttttcatatc tcttattaat ccaacaagca ctgacctatt
     5221 tcttgatgga atctcataac tatgccctaa agatgtaact atgcatacag gagcttcaaa
     5281 tccaggaact aatctattaa atccatcatt agaagctgtt acaattgggt ttaatacttc
     5341 ataattgtaa agaagtccca taagagcccc atatcctagt tcacttagat aatcttcttt
     5401 taaatctttt ggagcaaata gattttttat acttccatcc tttaacttcg cacttactcc
     5461 tacatgtgta tgtccaccac ttcctgctac tccatgaatt ggctttgctt taaatgtaac
     5521 ttctagtcca tgtgatgtaa atacatcctc tataacatct cttactaata attcattgtc
     5581 tgctgcttgt aatggtgtag aaaacttcca agaaacctct aattgctcca tagcatggtt
     5641 tgttcttcca tctatactaa tagaactttg tattcctccg acctctttgt gagccatttc
     5701 agggtttaca cctagctttt gaagtataat taaacttttc tctaaacaag ttcttataat
     5761 accatgagtt cttttccagt attgctcttt taggctttgt gatacatata atttctctaa
     5821 gtcagcttta tcctcaggag tattaaccca gaactcaagt tctgtagctg ctgttaacat
     5881 tatttcctct atatcatcta ctgaatctat tcctatgtta tttattacat gtggatattc
     5941 tctaaatatt tcatacattg actttttaaa gtacttatct gctttttgaa gtactcctct
     6001 agaacatact tttttattat catgtattaa aaatgcaggt atctttaatg ttccaacagg
     6061 tagacctact tcttcgtcta tatattccat attgtaatct acataccaat gacaactttt
     6121 atctggcatt aaatctactt tcgcattatt taatgtagct atattataaa gctcaacact
     6181 tgaaccatca gtctgtatag ctgattccaa aaatttatct atatcctcca aaaataattc
     6241 tacaggaatc ttttcatctg ttgcatttcc tcctaaatca acccccatta aggaaacaaa
     6301 tcttatattt ttattgcttt ttaggatttc tcttaattct tcattagtgt gtttatcttt
     6361 ctctattacg tataatagac tatccatttt tccactctcc cataattttt atgtatattt
     6421 tattttgtta aatacaagat aaatttcatt ttccttacat tcatgaattt ttcttgcaaa
     6481 ttattcgtgt tttattcgtg tttttattta tattattata tatcaagtat aaaaagttcg
     6541 caatattttt tattggaaaa aatattatac atacaatagt ttaacatttt tacaaatatg
     6601 gggtatacta aaataaaagt atttttattt gaaaatttaa ttttatttat aatataatct
     6661 atagtaaaag aaataataaa aaaatttagg agataaactt tatggaatac ataattttcg
     6721 atttggaatt taaccaagga tttgataaaa aactcaataa aactgtatcc aatgaaaagt
     6781 gtccttttga aattatacaa ataggagcaa ttaaattgga ttctaaattc aatataatag
     6841 atacgtttaa tagctatgta aaacctacaa tttataaaga catacatccc tttattagtc
     6901 gtatgactaa tattaaaaat tctgatttta atgattcgcc tactttccct gaggtctaca
     6961 acaattttat aaaatttata tcatcacaag accctatatt atgtgtttgg ggagttggag
     7021 atttaaagga attatacagg aatatcaact atcacaagct accatcaagt tccttaccta
     7081 aatcttatat taatatacaa caacatgcat ctaaatattt caataatcca gcaggaaaaa
     7141 gcattggcct acaaaatgct atttctatat tggaacttga tgaaaaaatg tcttatcaca
     7201 atgctttaaa tgatgcatat tatactgcta aagtttttat taagatatac aatccaagta
     7261 tagtcccaga tatatatctt tatacgagta taaaacctaa aactataaga tattctaata
     7321 aaaaaagagt tgattatgat aagttatttg atgaatttag gaaaatttta aatcgtgaat
     7381 taactaaaga cgagaaaaaa atcattaact tagcttataa tatggggaaa acaaatcaat
     7441 ttacattaga aaatgttaag caaagaaaaa acaaatagag tttatttaga taaaaaatag
     7501 gcatgtagat taaaaaacta gttaacttta aatttatatc tacagcccaa tttttatcgt
     7561 aattaatttt tattttatct ctaattcaac tggacaatgg tctgaaccta atatttcagt
     7621 gtgtatatcc gcacttacta atctatcttc taattttttg gatgcacaaa aataatctat
     7681 tctccaccct gcattatttt ttcttgcatt aaatctatat gaccaccaac tataaacgcc
     7741 ttctttatca ggattaaaat atctatatgt gtctataaac ccactgtcta ataatttaga
     7801 aaacttctct ctttcttctt tagtaaaacc tgcatttttc atattatttt taggattttt
     7861 taaatctatt tctttatgtg caacgttcat atctccacac acaattacag gcttatgatt
     7921 atctagtttg gttaaataat ctataaagtc gtcttcccat ctagttctat attctagtct
     7981 ctttaattca gattgagaat ttggcgtata tactgtaaca aagtaaaaat cttcaaactc
     8041 tagagttatc actctaccct ctttgtcatg ttcttctata tttattccat acattacttt
     8101 taaaggctct ttttttgtaa atatagcagt acctgaataa ccctttctct cagcataatt
     8161 ccaatattga aaatatcctg gtaaatctaa ctctatttgc ccttcttgca atttagtttc
     8221 ttgtaaacaa aatatatctg catctacttc tttgaaaaag tctaaaaatc ctttcccaac
     8281 acaagctctt attccattaa cattccatga tataaatttc atacttatac tcctcatcta
     8341 aacattgaca cactccccat agctaaagat gggggctttt caacaatttt attgtaaatt
     8401 cttaatttac ttttatttta acaatactat actataattt catctataat tactatttac
     8461 ttaataccta aaaatatatg ttttaatcaa tttttaactt ttttgttaaa tcttcagggc
     8521 tcattgaaga aacatacata tttgttccaa gttcaaatcc tccaaaatta tattttcttg
     8581 ggattatgtg catatgaaca ttaaaatacg attttgtttc aatctttgtt ggatgagtgt
     8641 gaatacataa gttaaatggc atatatccat tgatattgta taacttctta aacaactttt
     8701 caaatattgt agaaagctcc tgtatattat tatcatttaa ctcaccaaac tctattttat
     8761 ctttaaatat tattcttact tctccactat acttggttgc atatggtata agtaccaaaa
     8821 aagcttctcc attataaaca actcttctat ctaaacttat ttcattttct attaaatcat
     8881 catacaaaga gcttttattg ctgtcataat actcctttga aactaacagt tcattcgtaa
     8941 tttcaggtgg aataaaagac atagacaata tttgagcatg tggatgcatc aaagatgctc
     9001 ctgcctttct caagaagttt ttgaagatac taacatactc tactttatca tctttcgaca
     9061 aatctctaaa tctatttcta tacataaaaa aaacatcttc aaattctttt tgactcatgt
     9121 tataaaaact acgattatgt ttatcgctct ctatcattac ttcatgaaca ccatatacat
     9181 ctcttgccat atcatcaatt ataggaaatt tattgtagac agattttaca agccatcctt
     9241 cttcactttc tatttcaaat aatgcatcag ttgcatgagc ttcatttcct ttgcaaaatg
     9301 ggcattcttc atcatattca ttagtttctt catcttcatt tgggatatct gctgtatcta
     9361 aaggtctttt taacctatct gtagcaaata taacaacatc attagtaatt ggatctattc
     9421 ttaattcttt cataattaac acctcttagt taatagcctt taatataatt atatgatatt
     9481 tatcgtatct gttaaataaa tttataatat tttttataat tttgcatatt tattattata
     9541 tattttgtct atagctgaaa gattataact aaaaaatcac tatcagtgta tcagaatatt
     9601 atcttactat aaattaattt atgaatacat aatttagtgg cataaacatg ggtattttac
     9661 tataatttgt taaaagttta ttttaaagct atattaattt tttttgatga tttttaccca
     9721 taagtatgtt attattatct tgtaaagatg tcttgtattc aaacatacaa tttacataat
     9781 acaatatacg tatttaaatt aaataggggg tattaaaatg aattattcaa acagagttag
     9841 tgcaatgcaa gcctctccaa tcagaaaact tgttccattt gcacaagcag caaaagataa
     9901 aggaatcaaa gtatatcact taaacatagg acaaccagac ataaaaactc ctaaaggatt
     9961 ttttgatgct gttaaaaact ttgatagcga agtcttagaa tatgctacat cagaaggtat
    10021 tccagaatta ttagaagcac ttcaaaatta ctataaaact tacgatatga attttgaaaa
    10081 agatgaatta ttagtaacta atggtggtag tgaagcttta cttttcacta tgatggcagt
    10141 ttgtgaccca ggagataatc tattagttcc agaaccattc tatacaaact acaatggatt
    10201 tggtcaatct gtaaatgtag aagttaatgc tgttacaaca aaggcagaaa acggtttcca
    10261 ccttccatct aaagaagaaa tattgtctaa agtagatgat aaaactaagg ctataattct
    10321 ttctaaccca ggaaatccaa ctggtgcaat ttatacaaaa gaagaactta atatcctagc
    10381 tgaaatagct aaagaaaaag atttatggat aatagcagac gaagtttata gagaatttgt
    10441 ttatgatggg cttgagtata caagctgtgg taacttagag ggtgtacagg atagagttat
    10501 aataatagac agtgtttcta agagatacag tgcttgtggt gctagaattg gttctatcgc
    10561 ttgcaaaaat aaaggtctta tcgctcaaat acttaaatta tgtcaaggaa gactttgtgt
    10621 tcctacatta gagcaaatag gagctgttga gttatacaaa actcctgttt cttacttcaa
    10681 agaagttaat gaagagtaca aaaaaagaag agatgtatta tacaatgaat taatgaaggt
    10741 tgaaggtgtt atatgtaaaa aaccaactgg tgctttctat atagttgcta aattaccagt
    10801 tgaaaatgca gaagatttca caatttggat gttaaaagaa tttaacaaag ataatgaaac
    10861 agttatggtt tgccctgctg aaggattcta tgctactcca ggtttaggac gtgatgaaat
    10921 aagattagct tacatcttaa atgaaaaaga ccttcataga gctgctacat tattaaaaga
    10981 aggtttagag caatatgtag ctttacaaaa ataatttttt aaacttaatt tatataaatt
    11041 taatgatata aaatgtcccc ttttcagata ttatttgaaa aggggacatt tcttaattaa
    11101 tatattaatt aagacttaga cctgactatt tttcattttc tttatatagt gttggccagt
    11161 aatctttatt cactttaatt aaagcatcta aaacttgtct tgccttagtt ggagagatta
    11221 tagttctatt taatgttaaa gataaaatag ctttttcata agattgttca aagtatgcat
    11281 caactgctaa cttttcataa gctagttgac cttctaacag tcccttataa aatgttggta
    11341 tattacctac atgtaaaggt ctcggtccat tctttgtaag aatcgcagct acttctacca
    11401 ttgcatcatc aggtaagttg ttaatagccc cattattttc tgttataact atggctattt
    11461 ggtgtttgtt atgagctatt gcttctgcaa cttcaactat aaattcccca tgagcatcat
    11521 tatgcactaa attagaacct atagatgttc ctttttctat aacagattta cattcttcaa
    11581 atacacgctt ttctcttcca ttcataactt ctcttgctct agtacaattt atatttgatt
    11641 tagctacttt tttatcagga tatagataat actgtacata tgtattaggt aagtaatctg
    11701 gtacatcttc caacatatct ttaaccattc cataagtgtc taaccaagat ttatctcttt
    11761 gttctgcatc aacaggttgg aatccagagt ttgctactaa atcttttatc ttaggtacca
    11821 aatcttcacc atttttatca tacaaatgtg taaaccatcc aaagtgattt aatccaaagt
    11881 atacaggctc aaatgttctt gaatccatgt ctaacaatct tccataagac cttaacaagt
    11941 taactggttg gtcacacata ttaagtaatc ttttatcatc tggaaatact ctttttaatg
    12001 cttctgcaac aatagcagca ggatttgtat aatttaatat ccatgcttta ggagagtatt
    12061 ttcttacagt cttaaccatt tctatcatat cacctataga ccttaaccca taagcaaatc
    12121 caccagctcc acaagtttct tgaccaacca gtccaaattg tagaggaatc ttttcatcta
    12181 attctcgcat cttaagacca ccagttctca tttgaacaaa tacaaaatct acatctataa
    12241 atgcttcatc tgggtttgta gtatattcaa gtgttgcttt aggatattct tctttaaata
    12301 atatttttcc aaattcccct attaatcttt gacgttcttc tactacatct aacatagtta
    12361 ctttatttag aggaaatcta tcactcattt tacataaaga ctttaaaagt cctggagtcc
    12421 aagtactgcc tcctccaact atagctatat tgtatctttt tttcattgtc atattaatca
    12481 cccctgtttt ttattaacta acttgtaaga tgattataat atataaagtt ttctacatga
    12541 atacttttag gtaatcatta aaaatagttc aattttagta tttttttcaa aattgtaatt
    12601 tttttcatct attgtatttt tcaatgtatt tatgaaacat atattccata agagaaagtg
    12661 ctggcactct agacgtatag ttaacactat taaaattata ttgtggagaa gatacaaata
    12721 attttatatc agctatattt gataatgaat tgacacttat atttgtcaga gaaattactt
    12781 taacttgatt tgtttttgct atattacatg ccttaataac ttgagcagtc tctccagaca
    12841 tagatacagc aattacaagg tcatctttag taatatttga tgcagccaaa tacatgagag
    12901 ttgaatcatc atagtatttt acatttacac ctatagctga aaaacgttga gacatttcag
    12961 tacatactaa tctagaagac ccaacaccaa aaaaatatat atgcttagaa ttatgtattt
    13021 catttaaaca catatctata ttctctacat ttattaaatt ttgtgtatcc ataacatcag
    13081 taaaaatatc actttgactt ttagtaaact ccttatttct ttcatttcta tttgaatatg
    13141 aaaaaactaa aaagttttta aattcactat atccagaaaa gccgagcttc tgacaaaacc
    13201 ttactatgta tgcagtcgaa ttaaaagttt catttgctat atctttaatc tttttttgat
    13261 ttattttttc agaatttttt agaaaatagt ttaaaagaga tatttcattt gtagataatt
    13321 ttttttgatt tatatctaat ttgtctatta ggttcattta tacctccata ataatgattt
    13381 gtagttaaac aactttctca tatttattat ataaatatac actatacaac atttatctta
    13441 tgtttattat actacaaaaa agacaataca aattataaat tagtattgcc ttttaatatt
    13501 tacattagat agacattaaa tagacattgc aaaagaaact tcgtctctta cttgagatac
    13561 agatggccca taaattattt ggatatcttt atcagatact tttataacgc ccattgcacc
    13621 tgtatatttt ttaaaagcat tttcatctac tttactcata tctttaacta ctactcttaa
    13681 gcgagttata cagtttgtta tatcttctat attctcctta ccacctaaat atttaattat
    13741 ggataatgct ctcttagtag tttctccact ttgagctatt tctactttat ttgataaagg
    13801 ttctaaatca aaatttatat ctgttatttc ttctcgtcct ggagtttgta agttaaatct
    13861 aagtatcaaa accttaaata cattataata aaccagtgca tacactaaac caagtattat
    13921 agaataaatc cactttgtat ttggttgcat aactccaaat actactaaat ctattatgcc
    13981 accttgagta tttcctattg ctactccaaa tatatgcatc atcataaatg atatacctga
    14041 taaaaatgca tgtataacaa ataatattgg tgatacaaat aagaatgaaa attccaatgg
    14101 ttccgtaatt cctgttacaa atgttgctaa agcaccagct agtaatatac ctttaacctt
    14161 cgacctattt tcaggctttg cagttttata catggcatat gctgcagcag gaagaccaaa
    14221 taccattata ggtattttcc cttgagctaa aaatctagtt gcttctgttg aaaaactgat
    14281 tgtttcaggg tcacttagtt gagctaaaaa tatgttcaat gctccaaata cttcttgtcc
    14341 tcctacaact aaagaacctc ctacttcagt aaatctaaac atagcattta atatatgatg
    14401 taatccaaat ggtattaaaa gcctttcaag aaatccaaat acaaaggttc ctaaatatcc
    14461 taaactagct ataccaatac ctacactctg aataattgta tttacatatg gccatatata
    14521 tggtattatt atccccaaaa tagagaatac tactgttgtt attattggaa caaacctagt
    14581 tccaccaaag aaccctaaat aatctggcag ttttatatta tagtatttat tatgtaatga
    14641 tgctgttatt ataccaacta caattccacc aaatactcct aactctaatg tttgtatgcc
    14701 taaagttaca gcttgaccca tttctctcat catgtcaggt tcagcaagaa tacctctaca
    14761 ccctaacata taatttacac tagtgtgtaa tactaaataa ccaacaaaac cagaaaatgc
    14821 agaaactgct ttttcttctt tagcaagacc aacagatatt gatatagcaa agaatattgc
    14881 taagtttcca aaaagtactc ctgttatagc ttttgtaaaa cccaatattc cttgaataaa
    14941 attattttct aaaaaaggta tatcactaac tatattagga tttgaaaatg ctgaacttat
    15001 tcctaaaaat atacctgcag cagatattaa tgctattggc aataaaaatg attttcctaa
    15061 ctgtaaaaaa aactctttca ttttttgttt catttctatc cctccatttt taaattttta
    15121 aaacgttttc tatgtcataa ttatatattt ttattttgtt tttgaaactt tataatttgt
    15181 tttaaaatat ttttacataa atagtaattt tttcaaaact gtaaattttt tcaaacaaag
    15241 acatagtaac attgtgatat atagaaattt aaaaagagat tagaaaataa aattattgaa
    15301 tataaaatgg aaaaatataa attacacaaa gaggtatgag taggtactaa caaataagaa
    15361 aatgtataag aaattataaa aaagtacatc aagaaaattt aagatgtact ttttagattg
    15421 tatttagact tataattata tagttaaatg aataacaaag aattaatctt agtactatta
    15481 aatataaaaa ttttttatat tttttctatc taaattcttc tttgtttaat atatattgaa
    15541 gagtatttat tgcttctaaa tatttttttc tgtcttcttc agttaattta gaaagttttt
    15601 ctcctaaacc atcacttaat ttatccataa aatcagttgc tatcttatct ccctcttcag
    15661 taatgtcaat tagatatttt cttctatcaa ctaaatctct tttccttgtt acatacttct
    15721 tatcagttaa atcatcaacc atactagtta aactgccttt ttctatattt aacttagagc
    15781 ataaatcagt catacttatt actccataat tttttatgaa aactaatgct cttaattgtg
    15841 ttttatttac attataatct ggtgcaaatt tttttaggtc ttctaagtac agactagaat
    15901 aaatcttagg aaatgttttt actaaaaaac ttatggtatc ttttactata tagttcatat
    15961 aatcacctcg gttgttttaa aatttattaa attatctaaa taactttatt tcagtttaca
    16021 ttaaaaatat tgcaagtaca atagtttttg ctgaatgttt aaatttaatt agtaatctta
    16081 tcattttttt atttttattt atattatttc tattaaatat tatttatata gctattttat
    16141 taaattcatg gtatagtaag taaagaagaa ctcttcacag cgctgaagag aagacgctag
    16201 cccattaaaa tggctatctt atttataaat aagatagccg tttttaatta tattttatct
    16261 gtacaattct atactcttag gtccacatct acttttccta agtcttcctt ataatctttt
    16321 atagcaggtt ctacaacttc atttacaaat tctacaactt gttctggagc tctacctata
    16381 aagttttttg ggtccattat agatagtatt tcttccttgc tcattttaaa tgaatcatca
    16441 ttcattataa gttcaattaa gttattatcc ttaccttcgt gctttactct ataggcagct
    16501 ttcatagaat attctcttat tatttcatgt aattcctgtc tatctccacc tctttttaca
    16561 gcttccataa gtattgtttc tgtagccata aaaggaagtt cttcatttac acgtttattt
    16621 atcatatttt catatactac taaaccatca gttacattta tccctatttc aagtatagca
    16681 tctgctgcca taaatgcttg aggtattgcc aatctcttat tggctgagtc atctaatgtt
    16741 ctctctaacc attgagtggc ttgtaccatt gcagggctta tagattcaga gattatatac
    16801 ttagataaag atgctattct ctcacttctc attgggtttc tcttataagc cattgctgat
    16861 gaacctattt gatttttttc aaatggctct tctaattctt ttaaactttg taaaagtctt
    16921 atatcgttag tcatcttatg catactttga gctatccctg atagtatgct tattacttga
    16981 taatctaact ttctagtata tgtctgtcca cttactgcat aggaagaatg gtatcccatc
    17041 tttttacaaa ttttcttatc taactctttt actttttcgt ggtctccttc aaataagctt
    17101 aaaaaacttg cttgagtacc agtagtacct ttaactcctc ttaacttcat gttatctatt
    17161 ctatagttta aatcttccaa atcaagaatt aaatcttgag cccataaaca agctcttttt
    17221 cctactgttg taagttgagc agcttggaag tgtgtaaatc caagagctgg catatctttg
    17281 tacttaagtg caaattcttt taaattgttt attaaattta caagctttac tcttagaagt
    17341 tttaaagctt cattcatttg tataacatct gtattatctc caacatatgc agatgttgct
    17401 cctaaatgta ttattccttt ggctttttca cattgtaaac cataagcttt tacgtgagac
    17461 attacatcat gtcttacttc tttttctatt tttctagctt catcaaaatt tatattatct
    17521 atattagctc ttagctcatc taattgttct tcagttatat tgattcctaa ctcatgttca
    17581 ccttctgcaa gtgcaaccca taaccttctc catgtagaga atttaaattg tggagaaaat
    17641 atataactca tgtccttgct acaatatctg tctgtaagtg ggtttgagta agtagtatat
    17701 ttattatcta tcattatatt agcaccttcc ttaaatcttt tgtcgtgtga ttactgtgta
    17761 cacattagta cataaataat tatatataat tttacgaact ataacaagac ttttttattt
    17821 tttgttcaca tctatatata gtatttttta tttattacta ttttttattt atatccaaat
    17881 tatctttttc aattaagtaa tccactattt cactatcaac aaaatttaat acatacttat
    17941 atctaattgc atagtattta tcactccatc caagctgatt ttttacatca ctttcatctg
    18001 ccccaagcct taaacttatt gctgcaaatg aatgtctaaa atctcttgct gaatattgag
    18061 aaagtccagc taaatctaat gcttttttga ctataagtct gacatttcta tctgttatag
    18121 aattactctt ttgagttgta aaaataaaat catcactagg tattattacc tctggaagcc
    18181 cagaataatt cctgtaatct tctaaaagtt taaaaaagta tggatgtaat ttaactactc
    18241 tttcttttct tcccttacct aaccttacat atgagttatc gttttcatct accataaggt
    18301 ctttccactt taagctaact aattcattta ataaacaccc agtagtaact agacagacta
    18361 ttattactct atctcttata tttaattttg gtaggatacc tataagttta tttatttctt
    18421 gtatacttag aacatcatct tttgttttta ttcttgttac tgtgggtttt tctacatatc
    18481 taaatggatt tatatctatg tatccttctt tttttaagaa gttataaaaa ctatgtaaat
    18541 aactatatat cttttcacaa gttgatttcg catatttagt ctgtatatag tctacaaaat
    18601 ttttacaatc ttcttttgta gcataaatca attctttccc ttttaaaaat tctttaaaca
    18661 gtataacctt tgataaataa tcgtgtttcg tgtttggatt taattttgta gaaaaaacat
    18721 taaaagtttc atactcataa tcatctagta tttttgtact atacataata actcctttcc
    18781 ttttcataaa ataaaatagc tatactttta ctataccata tatttccccc tcaaatatac
    18841 cccttattca atagataact tttaagttta ttttgttata ttatgaaggt tttatgaaaa
    18901 tatcaatctt ccattgtgca ctacacagta ctatggtatt ataagttagt aaaaggtggc
    18961 atttttttat taatctatga ataaaatatg ctactataaa tttgtattgg aggtattttg
    19021 atttggaaac atttagtttg cttgaaattt ttaaggcagt cattttgggc atagttcagg
    19081 gaataacaga atggctacca gtaagtagta cagggcatat gatattaatt aatgagttta
    19141 taaaattgaa tttttcaaat acatttataa atacattttt agttgtaatt caatttggtt
    19201 caatactagc tgtacttgtt atatttttta gaaagttaaa tccttttgat agtgcaaaga
    19261 atattaaaca gaaaaaagaa acagttcgtt tatggcttaa ggttataatt gcagttatac
    19321 catctggagt tattggaata ctgtttgaag atgacataga cagagtattc tttaattcaa
    19381 cagtagttgc tattgctctt attgtctatg gtataataat gatagggctt gaaaagagaa
    19441 ataaaagacc taagtataaa gatttttctc aagttacata taaattagct ctttgtatag
    19501 gattattcca gtgtctagct ctcataccag gtacatctag gtctggttct actataatag
    19561 gtgcagtatt acttggtact tctagatatg tagcagcaga gttttcattc ttcttagcca
    19621 ttcctacaat gcttggagct agtgcattaa aacttctaaa agctggattt ggatttactg
    19681 gatttgaatg gcttatttta ggtgttggtt ctgtagtagc atttgttgtt tcaattgtag
    19741 ttattaaatt ttttatggac tatatcaaaa aacatgactt caaagtattt ggttattata
    19801 gaatagtctt gggtatagtg gttcttgctt atttctttct attataatgt attctaattt
    19861 aataacaaat gataaggaga aaagttatga gtgttaaaaa agaaatattt gattggatta
    19921 agtcaatagc tatggctatt gtacttgcat ttgtaattct acaatttata agaccttctg
    19981 ttgtaagtgg agaatcaatg tatcctactt tagatgataa agattatctg attttaaata
    20041 ggatatcata caaggttggt aaacctgaaa aaggcgatat tgtagttttt aaaaccaatt
    20101 tagttgatgg agaaacagga aagaaaaaag acttaataaa aagagttata gctactgaag
    20161 gtgacagaat aaaaatatca aattctaaag tgtatgtaaa tggaaaatta ttaaatgaac
    20221 catatataca caataactat acttctggag atatagatac tgttgttcca aaaggtaaac
    20281 tatttgcaat gggagataat agagaaaata gtaatgatag tagattccct gatgtaggta
    20341 tggttgatga agatgaaatt cttggtaagg ttatggtgag actattacct cttgataata
    20401 ttgggaaagt ggactaaaaa ataagagact gaattggtaa agtttatttt acaaatctca
    20461 gtctcttttt attttcttaa attacaaata tttttttact aaatatcttt tgattctgta
    20521 ataagtctta ttatatcact tgcttcttca ttaaaagttg ttactatact taggtagtct
    20581 gttgctctac tcatagctaa ataaacctta tttaaatctc ctacaaagtc atttacttga
    20641 taatcttgtt caatatcatt tatagtttga ttgtataaca tctctaattc acatactatt
    20701 accgctttat actctaaatt ctttattgca tatatatttg ctattgtagt tcctaccttc
    20761 ttacttatat ttgtcaaatt atcttctgca catatataag gaatttttgc ctcttccaat
    20821 gcttttttta acatatattg aaagtaaatg gtttttccac tttttaattt cttcttatta
    20881 taaggataaa cgatagcaac ttcagaataa tcaagacctt ttttacttat taaatactca
    20941 atttcccaga ttacagaact tatttggtca tcaaggtcac ttacttttat tatatcaaca
    21001 gacttatttc cacctctaag tgctttagta taaaagaaag tactaaattc tgtatttggt
    21061 cttaattcgt tgatatatga gttagaatta tttgaaaatt tatttgtaaa atctacaatc
    21121 tctttagctt gtctataatt tttacttaag ataatcttat catcaaattc tataccatta
    21181 tataattttt taaatatatt caatctatta gagatattca aggagttaca agaacacaca
    21241 ttaaatatga attttgtttt atataaaaac tcccttataa aatctatttc atactctaaa
    21301 aagctttctg cttcatcaat aaaaatagcc ttgaacatat ttttattttt tataatgttc
    21361 tgtgcttgtt ttacaagatt attaaaggtc ttttcgtaat cattttttaa catgttataa
    21421 tctacaatta aattgaattt tttagctagt ttaaatataa aagaactaaa tgtgtgaact
    21481 tctaaattgt tattatcttt gtataataac tctatcctct ctcgaagctc attgcacaat
    21541 tgttttgtat gtgtaaatat gactaattta tgatgtggat atacttttgc taattttata
    21601 gccctagaaa gcataattgc tgttttgcct ataccagaac caccttcaat aagtgtattc
    21661 ccatatttaa tagaaacaac atctttaatt tgaacgtctt ccatcatagt tgctgtatac
    21721 ttatagtcat cattgtagaa tgaaattttt ttaaattttt catttagatg aagtttatca
    21781 tttattatat agtattctga acatatgtct agtaaaaata aatttaagtt tatttcatca
    21841 ttctcatcat taagatactc ctccagcgaa cccttattca ttatatcttg aagcttattt
    21901 ttatctatta tattattatt aacaaattct tcaaattcat atgtttcctc aacttcaacg
    21961 tatggcataa tgtaaacgta gttataactt atatttctat tcttttgtcc catttttagt
    22021 ttcaaaagtt catattcctc attcataact tcaagtaact cttcttctaa aataaaaaat
    22081 atatcttctg ttgtatccat aaattttata aataaaattc ttccatcttt aacatacaac
    22141 aaatcggtat ttattccttt aaaaggagta acttttggca ctactttaac gtcctcattg
    22201 atattttcgt aaaatatttt ctcgaactcc ttcaatttta gggcgtctaa ctgagaattt
    22261 atatttatgt attggctcac ttacatta
//
LOCUS       DAGQWR010000068        22143 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.068, whole genome shotgun sequence.
ACCESSION   DAGQWR010000068 DAGQWR010000000
VERSION     DAGQWR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22143
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.068"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            444..1514
                     /locus_tag="MIW95_002169"
     CDS             444..1514
                     /locus_tag="MIW95_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151494.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGKLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1498..2367
                     /locus_tag="MIW95_002170"
     CDS             1498..2367
                     /locus_tag="MIW95_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBY3151495.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2411..3271
                     /locus_tag="MIW95_002171"
     CDS             2411..3271
                     /locus_tag="MIW95_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBY3151496.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSMEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLILQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYIPLIIPFMTS
                     SFGIFLLRQNFMQISDETIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3302..4606
                     /locus_tag="MIW95_002172"
     CDS             3302..4606
                     /locus_tag="MIW95_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY3151497.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INRDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4669..5931
                     /locus_tag="MIW95_002173"
     CDS             4669..5931
                     /locus_tag="MIW95_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBY3151498.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKVKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFDLKLDGVEKEAFDFIM"
     gene            6118..8088
                     /gene="uvrB"
                     /locus_tag="MIW95_002174"
     CDS             6118..8088
                     /gene="uvrB"
                     /locus_tag="MIW95_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBY3151499.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8107..10932
                     /gene="uvrA"
                     /locus_tag="MIW95_002175"
     CDS             8107..10932
                     /gene="uvrA"
                     /locus_tag="MIW95_002175"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBY3151500.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11191..13008
                     /gene="uvrC"
                     /locus_tag="MIW95_002176"
     CDS             11191..13008
                     /gene="uvrC"
                     /locus_tag="MIW95_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBY3151501.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVEAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13008..13937
                     /locus_tag="MIW95_002177"
     CDS             13008..13937
                     /locus_tag="MIW95_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBY3151502.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14151..15947
                     /locus_tag="MIW95_002178"
     CDS             14151..15947
                     /locus_tag="MIW95_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBY3151503.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQILSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLSPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16087..17868)
                     /locus_tag="MIW95_002179"
     CDS             complement(16087..17868)
                     /locus_tag="MIW95_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBY3151504.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRICMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17891..19777)
                     /locus_tag="MIW95_002180"
     CDS             complement(17891..19777)
                     /locus_tag="MIW95_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBY3151505.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19794..20291)
                     /locus_tag="MIW95_002181"
     CDS             complement(19794..20291)
                     /locus_tag="MIW95_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBY3151506.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLTTKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20609..22090)
                     /gene="cls"
                     /locus_tag="MIW95_002182"
     CDS             complement(20609..22090)
                     /gene="cls"
                     /locus_tag="MIW95_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBY3151507.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8525 a         2965 c         3697 g         6956 t
ORIGIN      
        1 ggtaaattta taaaaattaa aaaagtttta ttttttatgt ttaaaccttg taaatttggg
       61 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      121 aagacctcta tcccccatag aggtcttttt tattttcaaa ccaaattata taaaaaataa
      181 atagttttat atgactcttc ataaattaaa atcacattgt gatagcaaac catgaatatg
      241 aaaaatatag aaaattatag ctaacataag ctgtatatca tcttaacttc agtatattta
      301 acaaaaaatg tttataattt aacctttatt ttaataagtt tactatatat tcactttgtc
      361 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      421 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      481 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      541 cagtattggt aggtccaagt ggatgtggta aatctactac attaagaatg attgcaggtc
      601 ttgaagatat aacttcaggc aagttaaaaa ttgaagaaaa tgacgttaca aaaacggatg
      661 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      721 ttagagaaaa catagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      781 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      841 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      901 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      961 tgagaacaga attaatacaa ttacatcaaa aactaaaatc tacatttgtt tatgtaactc
     1021 atgaccaggt tgaggcaatg tcaatggcag ataagatagt tataatgaac aaaggaaaga
     1081 taatgcaaat aggttctcca aagaagatat atcaaaatcc taagaattta tttgtagctc
     1141 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
     1201 tcagaccaga aaaagcaaca ctagagagta taaacataga tggaaaaaat gatgatgtat
     1261 ttagcctatt tggaaaagtt gtgactaaag aagtgttggg aaatgaaact atatattgtt
     1321 tatctgttct taatagagaa ataagagtca aaacggaaaa tgatatattt gatattggaa
     1381 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1441 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aattaaagga gatatttatg
     1501 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1561 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1621 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1681 tttatacaaa ctataacgaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1741 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1801 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1861 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1921 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1981 tggaagactc ttggatacaa tatcttaata gtattatcag gtcttaaatc cataccaaac
     2041 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     2101 acattgccgc tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2161 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     2221 atgttatctt attatatata tgagtctgga tttatatact atgacatagg gcaagcgagt
     2281 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttagactc
     2341 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataagat ttatccaaaa
     2401 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2461 agcatcaaaa tcaatggaaa tattaattct attactttta tctgcaatat ttatattccc
     2521 ttttatatgg atgctatcga catcctttaa aaatccacaa gaaatgatgc aattaccacc
     2581 tactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2641 attttttaga tatttactaa acagtgtatt tgtaacagga agtgttttaa ttttacaatt
     2701 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2761 gttatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2821 ctatatgatg atgagtaagt ttggatttat aaatacttat attccactta taattccatt
     2881 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2941 aactattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3001 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     3061 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3121 aggagttgcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3181 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3241 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3301 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat taggttcttt
     3361 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3421 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3481 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3541 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3601 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3661 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3721 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3781 cataaataga gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3841 agagcttaag gattacgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3901 aacaccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3961 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta aattctggca
     4021 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     4081 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4141 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcctacc
     4201 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     4261 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4321 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4381 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4441 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4501 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4561 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4621 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4681 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4741 aactttaaat ctatactaga aaattgctcc atagtaaaaa atataaattg tgtttatccc
     4801 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4861 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtattca
     4921 aaggacataa aagtaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4981 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     5041 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagctc cagtaaggtt
     5101 ataatggacg gtatttacaa tagacattgt cattatattg attggaaatt agaacccaac
     5161 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     5221 atgctaatac atttagctac attggaccat acacgacata attatggctt atttaatgac
     5281 gaagtagata aagcattaaa aatgaatgat aaatggctag gagatattat tcaggctaca
     5341 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5401 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5461 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5521 ataatagtaa ataacttaga taggttaaca gaattaagag aactgcttga agaatttaaa
     5581 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5641 ggtgattttg aaatggtgat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5701 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5761 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaggt
     5821 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5881 gatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5941 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6001 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6061 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     6121 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     6181 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     6241 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6301 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6361 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6421 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6481 cattctgcta cagcatctat tttagagaga agagatacta taataatatc atctgtatct
     6541 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacca
     6601 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atacgaaaga
     6661 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6721 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6781 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6841 ttcccagcat ctcactatgt aacaacgcca gaaagaattg aaaaggcaat tgtagaaata
     6901 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6961 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     7021 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     7081 actttaatgg atttcttccc agatgattat ttgataattg tagatgaggc acatgtcact
     7141 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     7201 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     7261 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag gaccttatga aattcaacac
     7321 tcagagacaa tagcagaaca gataataaga ccaactggcc ttttagaccc aattgtagaa
     7381 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7441 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7501 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7561 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7621 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7681 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctaga
     7741 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7801 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatata
     7861 attcctaaaa ctatacagaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7921 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7981 gataaattaa aatcagagat gatggaagct gctcaaaact tacaatttga aagagcagca
     8041 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     8101 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     8161 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     8221 tcttctttgg catttgatac aatatatgca gaagggcaga gaagatatgt agaaagtcta
     8281 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8341 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctacg
     8401 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8461 gtgtattgcc ctacatgtgg tgaaaaaata agtcagatga ctattcaaga aatagttgat
     8521 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8581 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aagaaggatt tgtaagagtt
     8641 agagttaatg gtgagaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8701 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8761 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8821 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8881 gaagaattat ctccaaggat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8941 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     9001 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     9061 tacagtaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     9121 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     9181 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     9241 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9301 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9361 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9421 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9481 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9541 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9601 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9661 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9721 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9781 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9841 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9901 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9961 gcatcagaaa ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgc
    10021 ataacaggtg tttcaggttc tggaaaaagt acattaataa acgatatact ttacaaaggt
    10081 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
    10141 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
    10201 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
    10261 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gttttaatgt aaaaggtggt
    10321 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10381 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10441 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10501 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10561 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc gcaacgtata
    10621 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10681 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10741 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10801 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10861 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10921 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10981 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    11041 ttactcttca tggttaaaat tgttttcaaa tcaattatag aagggaatat ggactttaac
    11101 ttgtctaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    11161 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    11221 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    11281 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11341 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11401 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11461 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac gaatgaagat
    11521 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttggaccc
    11581 tataccaata taactgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11641 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11701 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11761 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11821 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11881 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11941 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    12001 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggtgta
    12061 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    12121 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    12181 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    12241 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12301 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaaacaaata
    12361 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12421 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12481 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12541 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12601 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12661 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12721 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12781 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12841 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12901 gtagaggcta taaaaaaagc tactatggaa gaacttttag aagtagatgg aatgaataaa
    12961 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    13021 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    13081 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    13141 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    13201 tttttggatt aatggatagt catattcgag aagaagtact agatagattt ttctcatatg
    13261 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13321 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13381 gactgtcaaa ctatttggat aataagttag caccacacac aacaattcac ggggtgctag
    13441 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13501 cagcattaga actgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13561 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13621 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13681 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13741 atagactagg actggataaa gaatacgaag aaatacttgg tgttaatgta gagaaattag
    13801 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13861 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13921 taatcgatac taagtagtac tgaataatag tgaactacac atcatttaat taaatattga
    13981 ttaggattat gtcctacacc ttataaatta aaatcaagtt gtatatatat aatgtttatt
    14041 aataagatat attatataag gataatttaa agtacagtgt tagattataa taatactaga
    14101 ttaattttat atttgagttt cattatataa caagaaagtt ggaatttttt atgggaatag
    14161 aatattttag taaactttta gaaagtagta ctgaaaaaga attgctctta agtaaaaagt
    14221 caaataacat aagtacagca agattaatat cttttttgat agtgatagca gggtttgcta
    14281 ttggatttta caataaaaat atagtaggta tttttgttgg agttttatca ataataatat
    14341 ttatagcact cttagttatt catagtaaag taaaagaaga agaaacatat tttaaaagta
    14401 agagcgaagt attaaataag tatattaaga gatttggaga tggttggaag gaatttaaaa
    14461 tagatggtaa agaatactta aaagatgaaa attcgcaagc aaaagattta gacttatttg
    14521 gaagagcatc attgtatcaa tatatctgtg tagctaatac atcttatggg aaaaattttt
    14581 tagccaaata tttgtggaat gaaaatccag atgagaatgt aatcttagaa agacagaggg
    14641 ctataaaaga acttttatca aaagatgatt tttctattca tattcaaata ctcagtaata
    14701 taattggtaa agagcaaaaa aataattcag atagtagtat agaatcgttt atagagtatg
    14761 gagaaaataa gaatgtgtat ataccaaaat ggatgcatat ttttacttgg ggtctaccaa
    14821 ctgcaacaat cttatctttt atattttgta tgttaggatt tttacctgtt ttacctgttt
    14881 ttttactttt tataatccag ttagggttta gtggttttgg atatccaaga cttatgcaaa
    14941 ctctttctcc attattttca tttagtagaa gtatacaagt atatgaaaaa atgtttgagg
    15001 ttttagaaaa agagacgttt gagagtgcat atttgaaaga attacaagat aaactttcta
    15061 agggaagtgg agtttcaaga ggtgttaaac aattaaattc aataggaaat gctgtaaacc
    15121 taaggtataa tcagatacta tatataattg cctgtggtgt tttgatgtgg aactatcatt
    15181 gtgcagaagc tttggagcga tggaaaggta tatatggaaa tcaaattaga gattggtttg
    15241 aatctattgg agaatttgaa gctctaataa gtttaacagt tatttctcat gtaaaggaaa
    15301 atacatgttt tccagtaata aaatatgaag atactccaag attgaaagtt gaagaggtat
    15361 accacccatt aatagcagaa aaaagtgtca tagcaaattc gatacaatta aattctcaga
    15421 cttgtattat cacaggttca aatatgtctg gaaagacaac atttttaaga agtatcggtg
    15481 taaacttagt gctggcatat gcaggtgctc cagtatgcgc taagagtttt gatgcgacct
    15541 gtatggctat atttacttct atgagaatac aagatgatgt aagtcaagga atatcaactt
    15601 tctatgctga gattcttaga ataaaatcaa tgattcaata tagtttaaaa gaactgccaa
    15661 tgttggttct agtagatgaa atttttaagg gaactaactc tgctgataga attattggag
    15721 caagtgaggc agtaaagaaa ttatcaaaac cctgggttat aagtatggtg acaacacatg
    15781 actttgagct atgtgacctt tcaggtagtg gagatgttga agttgtaaac tatcatttct
    15841 cagagtacta tgtagacgat aaaattcatt ttgactataa gataaaagat ggaagatgta
    15901 aaactacaaa tgcaaaacaa cttatgagaa tggcaggtat tctttaatta atagataggt
    15961 taattaaaga ataagtctat ttagaaatat aatttaattt gtgaaattaa taaggattgt
    16021 cttaaattac gtaaaagtat tttaagacaa tcctttttta agaataaatt ttatactttg
    16081 ttatgactac ttagcttctt cacaagcaca ttcttgtgga cactctccat agacacaact
    16141 cttatcaaag taatgagtat gaaggtattt atgggctaca tcactatttg gttctccaaa
    16201 gaagtcttta taaatagctt gtaaatcagg attctcatga gatttacgaa tagccttatt
    16261 tctatctatt tcatataacg catttgcacg tctatcaact atttcagtat taccatggtg
    16321 ataaggttga ccagcacctc caacacatcc accaggacat gccataactt ctatgatatg
    16381 gtatttacat tctccacttc tgaccttatc cataatcttt ctagcatttt taagtgtatt
    16441 tacaatacac acattaactg tagttccatc aacatctata cttgcttccc taattccttt
    16501 aagacctcta acattagtga agtttacatt atctaaagtc ttattagtta tcttctcata
    16561 tgaagtacgt agggcagctt ctaaaactcc accacttgct ccaaatatag atgcagcacc
    16621 tgtagatttt cctagaggat tatcaaaatc ttggtcttga agattaggta aatcgatagc
    16681 agcttctttt atcattttag ctaactctct agtagttata gaaaggtcaa catctagtat
    16741 tccagattca cttaattctt ctcttgaagc ttcatatttt ttagccacac aaggcattac
    16801 agatacaaca taaagttctt caggattgat tcctaatatt ttaggtgcat agtagttttt
    16861 agctatagaa ccaaacatac cttgaggaga cttacagcta gatgctaagt ttaacttgtc
    16921 tggataattg tgttctaaga agtttaccca tgcaggacaa caacttgtta atataggaag
    16981 attttcacct ttttggattc tttctataaa ttcagtagct tcttccatta tagtaaaatc
    17041 tgcaccaaag tttgtatcaa atacatgttc aaatccaagt gcttttaaag cagctaccat
    17101 ttttcctgta gagattgacc ctggttctaa tccaaactct tcaccaatag caactcttac
    17161 agcaggagca acttgcacta ctatagtctt ttctttttta tttaaaacat cccaaagttt
    17221 aggtacatta tctacttctg ttaatgctcc agttggacag acagatatac attgaccaca
    17281 gaatgtacac tctgtttcaa ccatatcaga gttaaagaat gtactaacta aagtattaaa
    17341 tccacgatta actccagata atgctcctac tgtttgtatg tcattacata cagtctcaca
    17401 tcttctacat aatatacact tagaatggtc tttaactaaa gatttagtag aagtatcttt
    17461 acctgcaaaa gatttggaac cttgatatct tatttttctt actcctaaat cagcagctat
    17521 agtctgtaat tcacaatctc catttttttc acagacaaaa caatcttgtg gatggtctga
    17581 taacaataat tcaacaatag ttctacgagc atttaatgct ttagcactat tagtctgaac
    17641 tttcatacct tcctttataa ctgttccaca agctggaact aagcctctct ctgtctcaac
    17701 catacaaata cgacaagaag cacaagtgtc aagcttatct atttcgttca tatgtaaatg
    17761 acaaaggttg tgtattttta tatttattaa tttcgcagca tctaagatag aagttccact
    17821 aggtgctgaa acgtgtttgc cattaatagt taaattaact aaactcattg ttattcctcc
    17881 tccaaataag ttatttcttg attatagcgt taaatgtaca attatctata caagcaccac
    17941 attttagaca cttagaagta tctattgtat gttgttcctt aacagaacct gttatacaac
    18001 cagcaggaca cactctagaa cattttgtac aacccttaca gtcatctgtt ataaagtaag
    18061 aaagtaagtt ctgacattta ccagcaggac atttcttatc tacaacatga gctatatact
    18121 catctttgaa atattgaaga gtacttaaaa cagggtttgg agcagcttta ccaagaccac
    18181 ataatgaagc agtttgtata gtttcagcaa ggttttctag gtcatctaaa tcttgtagag
    18241 taccattacc ttctgtaatt ttagttaata tttctaataa tcttgtagta cctatacgac
    18301 aaggtgtaca tttaccacaa gattcttcaa cagtaaactc aaggaagaac ttggctatat
    18361 ctaccataca gtcagattca tcaagaacaa gcataccacc agagcccatc attgaaccga
    18421 tagaacttaa tgaaccaaag tctataggag tgtctaaatg ttcagttggt atacatccac
    18481 cagatggtcc acctgtttga acagccttaa atttcttacc ttcagggatt cccccaccaa
    18541 tttcaaatac aatatctctt aaagtagtac ccattggaac ttcaacaagt cctacatttt
    18601 ctacttttcc accaagagca aagactttag taccactaga atcttcagta cctaagttgg
    18661 caaaccaatc tccaccctta agtataattg caggtatgtt agcaaaagtc tcaacgttat
    18721 ttaagcaagt aggagatttc catagaccac tcttagaaga actataagtt ttcattctag
    18781 gttcaccacg tctaccttca attgaatgca ttagcgctgt accttcacca caaacgaaag
    18841 cacctgcacc atattttaat tctaatttaa agttaaatcc agtgcctaag atattttcgc
    18901 caagtaaacc gtatttttca gcttgagcta tggctacttt taatctttct atagattttg
    18961 gatattctgc tcttatgtat atgtatcctg tatcagaacc tatagcgtat ccacatattg
    19021 ccatagcttc tagtacacta tgagggtcac cttcaagtac agatcgatcc ataaatgcac
    19081 ctgggtcacc ttcatcagca ttacatacaa caaacttttt atctgttgga ccagctggat
    19141 tttttgaagc agcttcccat ttagagccag tagggaaacc tgcaccacct ctaccacgaa
    19201 ggccagaagt ttttacctct gcaataactt cttgaggagt aagagaagtt aaacatttgc
    19261 ctagagctaa ataaccatca ttggcaatat attcttctat actatctggg tctataagac
    19321 cacaattttt taaagctata cgtaactgtt ttttgtagaa agacatttct tcttgttctt
    19381 caactttttt acttaaagat tcttcctcat ataataattc ctcgacaact gtatttctaa
    19441 ttaagtggct ttgaactatc ttttcagcat cagatggctc aacttttacg tagaaaacgt
    19501 tatcaggata aacttttaca ataggacctt gagcacagaa accaaaacaa cctgtcaaac
    19561 gaacactaac tttatcttgg attcctgatt tctttatttc attttctaat tcactaacta
    19621 tttctagact gtttgaagag gtacatccag tatcacaaca aacaagtaat tctcttctta
    19681 atacattttc cttagaataa ttttttctta agctaagatt aggtttcagc tcatcagcta
    19741 aaacttttaa ttcatcaaat gaatttactt tacgcattat ttattcaccc ctcttaacaa
    19801 ttcaattcta attctctata agtatctaat atttcgctta cttggtcagg tttcacttta
    19861 ccataaactt taccatttac agtaacaact ggagctaaac cacatgcccc caaacatctt
    19921 aaaccctcta aagaaaattt aaaatcacta gtagtttctc caacttttat gccaagctgt
    19981 ttttcaaaag cacttaagat tttgtcagca ccttttacaa aacatacagt tcctagacag
    20041 acactgattt tatacttacc tacaggttct gtagtaaaat aagaatagaa tgtaacaacc
    20101 ccatatacct ttgcaggagc tactccaagt tttctagcaa catgaagttg aacttctttt
    20161 ggaagatatc caaatatacc ttgggcttca tgtagaactt gtattaacgc accttccttt
    20221 gttgtaagag agtcaataaa tatatctagt tcgtcaaata attgcttgtt gtgtgaaata
    20281 aaatcgcaca taacaaatcc cccttttctt tttaaagtta ttatttttca taaaataatt
    20341 aaccatgagt tgaccccaac attatgtggg tgaacaaaac cagaacaata aagaaagaat
    20401 aatatacaac aatttcttta tttataaaaa caatgcaaaa aagttaccta ttaaaaataa
    20461 aaactatact agtctattat aatataaaat aattcagaat agttaaaaaa aataaaaaaa
    20521 atgagctaaa ttcaataaaa aaaaaataat acatataaaa aactttaaaa aataaaccaa
    20581 aatgtaaaat ttttataaaa taaaagattt ataaaactgc tgataatagt cttgaaattg
    20641 attccttgat ttttatataa gtagacctag aattatatat atctaatgtt atttccttag
    20701 aatttaaaat atcattttca aatataattc tttgttccaa tgcttttttt gaagagtaca
    20761 taaatgcatt aacttcgaaa ttaagctcaa aacttctgat atccatatta gctgttccta
    20821 tagagcatat agagtcatct ataactattg tcttagcatg taaaaatgca ttttgaccat
    20881 aagtgtagat tttagctcca aactttaaaa gttctccagc atatgaatat gatgcccagt
    20941 atacaaaggg atggtcaggt ttagaaggaa tcataactct cacatcaaca ccagataaac
    21001 aagctatttt taaagtatct ataaaagttg agtcaagtat taaataagga ctctgtatgt
    21061 atatatactt tctagctttc tgaatcattt taaggtaacc atatttaatc tcatcaagtt
    21121 ctgatatatc tggaccactt gatactatct gaatcccaat attttcagaa ggatgattag
    21181 aatttgtatc ttcttctata aagtactttt ctaaatctaa atcttcttta gttgtatatc
    21241 tccaatcaag gatgaatctc atatttaggt caattacgca atcaccagta agcttagtat
    21301 gtgtatctct ccatagacca aattttggat ttctacctaa atattcatca ccaacattat
    21361 atccacctac aaatcctacc ttaccatcaa tgacaacaat ttttcggtga tttctatagt
    21421 ttaagttaaa gtttacaatt ttcaagaatg atgggaagaa gcttccaact ttaacaccac
    21481 tttctttaag tctggaaagt gtactatttt ttagtgttct accaccaact gcatcaaata
    21541 aaagtcttac ttcaacacct tctttagctt tgtctataag aatgtctatt atttcagttc
    21601 cgattttgtc atctttgaaa atgtaaaatt gtatgtttat atatttttta gcttttttaa
    21661 gttcttcaag aagactgtta aaaaattgac tagattcagc ataaatccac acatcgttat
    21721 tattggtata atgagcatta tttgaattag ccaatgcata aatcatatcc ttatgttgat
    21781 gtatatctga atcaatttca gatgtagatt ggagtttaac ctgagtatct agtatattac
    21841 ttttgattat cttatcatcc ttttctttca atctaaacat attgtttttt gaaatatttc
    21901 tgccaaaagc tatataaagt atgaatccaa gagcaggaat caatactaat ataagaagcc
    21961 atgttaaagt tgtatttatg tttctttttt ctctaaagat aaggtttagt ataaccatga
    22021 aattaattat atatattgta gtaactgcta tttcatatat tgacatttcg aaaaatgata
    22081 tatcaaacat aaaattccct ccttcaaata tttattaaaa taagtaatta tatataataa
    22141 cac
//
LOCUS       DAGQWR010000069        21854 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.069, whole genome shotgun sequence.
ACCESSION   DAGQWR010000069 DAGQWR010000000
VERSION     DAGQWR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.069"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            293..1744
                     /locus_tag="MIW95_002183"
     CDS             293..1744
                     /locus_tag="MIW95_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBY3151508.1"
                     /translation="MNFLNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1964..2425)
                     /locus_tag="MIW95_002184"
     CDS             complement(1964..2425)
                     /locus_tag="MIW95_002184"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBY3151509.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGRYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(2531..3724)
                     /locus_tag="MIW95_002185"
     CDS             complement(2531..3724)
                     /locus_tag="MIW95_002185"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBY3151510.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(3747..4412)
                     /locus_tag="MIW95_002186"
     CDS             complement(3747..4412)
                     /locus_tag="MIW95_002186"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBY3151511.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(4446..5549)
                     /gene="ribD"
                     /locus_tag="MIW95_002187"
     CDS             complement(4446..5549)
                     /gene="ribD"
                     /locus_tag="MIW95_002187"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBY3151512.1"
                     /translation="MNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNIIGK
                     GYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKRVVI
                     STLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKSAVS
                     LDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQEKVS
                     HPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSKNNL
                     VDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPTFVS
                     GEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(5681..5802)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5991..8474
                     /gene="recQ"
                     /locus_tag="MIW95_002188"
     CDS             5991..8474
                     /gene="recQ"
                     /locus_tag="MIW95_002188"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBY3151513.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDLKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GANILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVMLDSYLENLKNNIIAE"
     regulatory      8562..8673
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8815..10116
                     /gene="thiC"
                     /locus_tag="MIW95_002189"
     CDS             8815..10116
                     /gene="thiC"
                     /locus_tag="MIW95_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBY3151514.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            10170..10364
                     /gene="thiS"
                     /locus_tag="MIW95_002190"
     CDS             10170..10364
                     /gene="thiS"
                     /locus_tag="MIW95_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBY3151515.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            10357..11160
                     /gene="thiF"
                     /locus_tag="MIW95_002191"
     CDS             10357..11160
                     /gene="thiF"
                     /locus_tag="MIW95_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBY3151516.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            11223..11993
                     /locus_tag="MIW95_002192"
     CDS             11223..11993
                     /locus_tag="MIW95_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBY3151517.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            12010..13113
                     /gene="thiH"
                     /locus_tag="MIW95_002193"
     CDS             12010..13113
                     /gene="thiH"
                     /locus_tag="MIW95_002193"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBY3151518.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYEFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            13114..13803
                     /locus_tag="MIW95_002194"
     CDS             13114..13803
                     /locus_tag="MIW95_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBY3151519.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            13991..15385
                     /gene="yfcC"
                     /locus_tag="MIW95_002195"
     CDS             13991..15385
                     /gene="yfcC"
                     /locus_tag="MIW95_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBY3151520.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            15902..17269
                     /locus_tag="MIW95_002196"
     CDS             15902..17269
                     /locus_tag="MIW95_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBY3151521.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            17294..17902
                     /locus_tag="MIW95_002197"
     CDS             17294..17902
                     /locus_tag="MIW95_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBY3151522.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            17950..18321
                     /locus_tag="MIW95_002198"
     CDS             17950..18321
                     /locus_tag="MIW95_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBY3151523.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            18500..18838
                     /locus_tag="MIW95_002199"
     CDS             18500..18838
                     /locus_tag="MIW95_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBY3151524.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            19305..19808
                     /locus_tag="MIW95_002200"
     CDS             19305..19808
                     /locus_tag="MIW95_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBY3151525.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            19855..20814
                     /gene="moaA"
                     /locus_tag="MIW95_002201"
     CDS             19855..20814
                     /gene="moaA"
                     /locus_tag="MIW95_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBY3151526.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            20818..21288
                     /gene="moaC"
                     /locus_tag="MIW95_002202"
     CDS             20818..21288
                     /gene="moaC"
                     /locus_tag="MIW95_002202"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBY3151527.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDYMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            21324..21755
                     /locus_tag="MIW95_002203"
     CDS             21324..21755
                     /locus_tag="MIW95_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBY3151528.1"
                     /translation="MAKVVSINISEKKGVIKVPVPCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         8441 a         2353 c         3761 g         7299 t
ORIGIN      
        1 ttgataataa tattaaacga caaacgaaaa aatataccct ttaaactaga atttattgta
       61 tccaataaca attataaaat attaaatata tctatattat aaaaataata aaaaaaggtt
      121 tgatttgaat ataaaactgc aatatattaa aaaaattatt cattttaaac cttgtaattt
      181 gattttataa atattataat taaattaatg aatttttaac aaggaattat tcattttttt
      241 gattttaaga tttattgaga aaacaaaact aaataggggg gtaattaata taatgaattt
      301 tttaaatggt attttaacga gtattaacaa tgcagtagta tttgtaaaca attacttatg
      361 gtcgtatatc ttaattataa tgttagttgt aattggtatt tactttacta ttaaaacaaa
      421 ttttgtacaa ttcagatatt ttatagaaat gtttagactt ctgggtgatg gtacagcgaa
      481 taaaaatgca aaaaaggaag gaaaaatctc ttcatttcaa gcattttgta taagtacatc
      541 ttcaagagtt ggaactggaa atatagctgg aatagcaatt gcagtggtag cagggggacc
      601 tggtgctgta ttttggatgt ggcttatagc attaattggt tctgcatcaa gctttgttga
      661 gagtacatta gctcagattt acaaagtgaa agatggtcaa gcttttagag gtggaccagc
      721 gtattatatg gaacaaggtc taaataaaaa atggatgggt attttattct caatacttat
      781 aactatatgt tatggttttg tatttaatgc agttcaagca aatactgttt cacttgcatt
      841 taataatgca tttggaataa gtaaaatggc tatgggaata ggtcttggaa ttttaactgc
      901 aatagttatt tttggtggag tgcatagagt tgcaaaagtt tctgaaataa tagttcctgt
      961 atttgcagga ttatatatat tagttgcttt ggctatagtt gtaatgaata taacagagat
     1021 accaagtgtt atagcattga tagttgaaag tgcatttgat tttaaaggaa tggcgattgg
     1081 tacatttatg ggagttgtaa tgactggagt gaagagaggt ctattttcta atgaagcagg
     1141 tatgggtagt gctcctaatg ctgctgcaac agcacatgta actcatcctg taaaacaagg
     1201 tttaattcaa tctttaggtg tttttacaga tactataata atatgtagtt gtacagcatt
     1261 catggtatta ttatattcag gttatagtag tagtggagca actggaattg aattagctca
     1321 agaagcttta actcatcata taggaccagt tggaaatata tttatagcag tttgtatatt
     1381 cttgtttgca tttagttcta tagttggtaa ttactattat ggtgaatcaa atatggagtt
     1441 tatgagtgga agtaagacta aacttaatgt atttagagtt tttgttgtag gcatggtttt
     1501 atttggttca cttactcaag tagatgtagt ttggaatctt gcagacttat ttatggcatt
     1561 gatggcgata ataaacttaa tagcaatagc acttttagga aaatatgcat ttattgcttt
     1621 aaaagattat acaagtcaaa aaaaatcagg aataaaagac cctgtgttta ttgcagatga
     1681 aatagaaggc ctagaaaagg tatcagagtg gcatagggaa ttatctgagg aacatttagg
     1741 ataaaaaata gtataaaatt ataaaaccat tttgaaagac tagagtatgt atatgcttta
     1801 tatgaatata atattgaatt aatttatatt actggaattt ttaattccag taatattttt
     1861 ttattatata atttagcttt attaaaagtt tttattatta aaaatagctt atttatcgta
     1921 aagtgaatga tattggtttt ttaattatta tgaatgttgg tatttaattt aaagacttaa
     1981 ataaatttgc catttctagc gcattcatag cacaatcata acctttattt ccagcttttg
     2041 tacctgctct ttcaatagct tgttcaatat tttctgtagt tacgattcca aatataacag
     2101 gtaattcact gtctaaagat acttttgcta tccctttaga gacttcacta catacataat
     2161 cataatgaga agtagcccct cttataacac agcctaaaca gattatagca tcatacctac
     2221 cacttttagc cattttttga gcaacaagag gaatctcaaa agctccaggt acccaaacta
     2281 tttctatatt ttctggagaa acgtcatgtc ttaaaagaca gtcttcagct cctgataaaa
     2341 gttttgatgt tataaattca ttaaaacgag aatttattat acctattttt aaatcttttc
     2401 caataagttt tccttcatat atcattttta tttcctccaa ttttatttat gttatagatt
     2461 tatatttata aatatcttta attttaatta taaaaaacta cttagtagaa aaatatcatc
     2521 atataatcat ttatataata tctaaaagat gtcccatttt atctttttta gtttttaaat
     2581 atagcttatt aaagtcatta atttcttcat taatagggat tctattttcg atttttattc
     2641 catatttctc taattgattt attttatccg ggttattact taaaagattt atacttttta
     2701 tgtttaaatt ttttaatatt tgagcagcca tatagaaatc tctcatatct tcttcaaatc
     2761 caagcattaa atttgcttca acagtatcgt aaccttcttc ttgaagttta taagccttaa
     2821 ttttatttaa aaggccaata cctctacctt cttgcttcat ataaattaaa actccacttc
     2881 cattttccac aattttcttc atagaagatt ctagctgtaa tccacaatca cattttagag
     2941 agtggaacac atctcctgtt aaacattcag aatgaagtct aactagagta ttttctatat
     3001 ttatatttcc ataagtaagg gcaatgtgtt cttcatttga ataagtatct ctatatccta
     3061 tgatttcaaa atttccatat tttgttggaa ggaaagcact agatactttt tctatagtag
     3121 tttcatttat tttgcggtat tctataaggt cttttatagt gatgattttt aaattatgct
     3181 tttctttgaa aatcattaaa tcatcaactc ttgccattgt accatcatct ttcattattt
     3241 cacagataag accaatctct ttaaaacctg caagtctagc aaggtctact gaggcttctg
     3301 tatgtccatc cctgacaaga actccaccag tttttgctat taaaggaaat atgtgaccag
     3361 gcgttttgaa atctttactt gtagaatttt catcacagaa caattttaca gtttcagctc
     3421 tttcatatgc agatatacca gttagagtat ctgctccatc tatagtaaca gtaaaggctg
     3481 tttgaaatgt atctgtattt tctctaacca taagaggtat attaagactt ttaaattttt
     3541 tttcagtggc aggtagacaa attagacctc taccatatgt agccataaaa tttattgatt
     3601 caggagtagc catttcagct gccatcaata aatctccttc attctctcta ctttcatcat
     3661 caactacgat taccattttc ccatttttta tatcttcaat agcttcttca atagtattaa
     3721 acatgtaatt cactccttta ttatttttaa aaaccatttt taaataaaaa atctttatct
     3781 atatttgatt tatggttact actattagaa tttaattcct tgtagttatt agccataaaa
     3841 ttattaacat actttccaat tacatcgctt tctaaattaa caaaatctcc aacattcttt
     3901 gtcaagagaa tagtgttaat tttagtatga ggtataatag aaaccttaaa tatttcatca
     3961 tctaaatatg atatagttaa acttacacca tcaatagcaa cagaaccttt gagtatcatt
     4021 gaagataaaa gttttttatc aggtcgtata cttactaaaa ctgcattttc atctttttca
     4081 aatttgcaga tagttccttt accatcaaca tgacctgtta ctatatgacc accaaatctt
     4141 gaagaaagac tcatagctct ttcaagattt accttatcat ttgcatttaa taaacctaaa
     4201 tttgtggaac gaattgtctc catcataaca tcggctgtaa attcattttt tccgaggttt
     4261 gaaacagtta gacaaactcc atttacggct atactatctc caagatttgt gccatctaat
     4321 attttatttg atagtatggt tacttttcca ctcttaccat tagcagtaat ttttctaagt
     4381 attccaactt cttctactat gccagtaaac atcctaacct cctatatgaa tgtattaaca
     4441 aaaatttaat ttaaaacatc tgattctata agaatatcgc catctatcaa agttacatta
     4501 ttgattgtta actgtgttga atctgacaaa tgatttattc cttctccaga aacgaatgta
     4561 ggtgcatctt taccacctat aatcttgggt gatagaaata atttgacttt atcaactaac
     4621 ttatttctaa agagactgtc attgagagta gcaccacctt caactagaac agaatcaatg
     4681 tttaactctc ctaatttata cattacaaat tccaaatcta ctaagttgtt ttttgaagga
     4741 gaaattattg tttcaacatt attttctttt aacatagata attttatact ttctttgcaa
     4801 catgtgaaaa ctatagtttt actggtttta tctttaacca aattactatt aagtggcaac
     4861 tttaggtgtg tatctattac tattcttgta ggatgagaca ctttttcttg attgagcctg
     4921 caagttagta aaggattatc gttcaaaact gtatttatgc caaccattat actttggtat
     4981 ttatttctgt atttatgagt tagatacctt gatgattcat tagatatcca ttttgattct
     5041 agtgattttg tagcaatttt accatctaga cttactgctg attttacaat acaaagaggt
     5101 cttttgtttt ttatatgata aaaaaatgcc tcatttaaat ctcttgcttc atcttctaat
     5161 attcccacag taacatcaat attgttatct tttaattttt ttactccatt accacaaaca
     5221 agtggatttg ggtctaatgt agatattaca actctcttaa tcttattttg tataatttta
     5281 tctacacaag gaggcgtttt cccataatga gaacatggtt ctaagtttac ataaagagta
     5341 gagtctttta gactttgttt agcactgttt atagcattta cttcagcatg attagagcca
     5401 aatttctcat gatatccttt gcctattata ttactatctt taacaattac acacccaact
     5461 aatggatttg gatttacaaa gccttctcca tttttagcca attcaatagc ttttttcata
     5521 taataaatat ctttttcttt ttgattcaca atatgtacac ctcctgttta attggataat
     5581 ttttaaacaa aaaaccctga gaattaactc agggtttaga ataatctaca ataaatgttt
     5641 aataaaagta gaagaaaatt gctctacaaa aacactcatt aaatatcttc ttccatccag
     5701 actttactgt cggctttgga atttaaccaa atcaaccaag ttttaccttg gctcgcgggc
     5761 tataccgccg attgggaatt tcaccctacc ccgaagatta agtattaaat ttatatttag
     5821 aatgtatcat aaattatttt actttgcaaa ctattttttt aaaaaatata tgttaatata
     5881 taatatagaa tgtctataaa ctttgaaata ccacgattgt gaatataaaa ataaaaattt
     5941 gaacataatc aggaaagaag tctaattaat ttaaattagg agagtaatat atgaataaaa
     6001 aacttttaga aatactatca aaatactatg gatatacaag ttttagaaaa ggtcaggaat
     6061 ctataataaa ttctatctta agcgaaaaag atgtgttagc tattatgcca acaggagggg
     6121 ggaaatcaat atgttatcaa attccagctt tgattttaga tggaatgaca atagtaattt
     6181 ctccacttat atcacttatg aaagaccaag tagatgcctt aaagactatg ggaattagtg
     6241 ctgcttttat aaatagttca ttaagcagca aagaatttaa tgaaatatta aataatatta
     6301 gaaaaaataa ttataaaata ctctatattg ctccagagag attagatgca caagagttta
     6361 ttgagctaat aaataataac aatgtaagtc aagttgcgat agatgaagca cattgtgtat
     6421 ctcaatgggg gcatgatttt aggttgagtt atagaaggat ttctgatttt ataaaaaatt
     6481 tgcctaaaag acctatagtt acagcattta ctgcaacagc ttcagaagag gtaagaactg
     6541 atataataaa tttactgtgt ttagaaaatc cagattatta tataactgga tttgatagag
     6601 agaatttatc aataaatata gtaaaatcat ctagtaaaaa taagtatatt ttagattata
     6661 ttcaaaatca taaaaatgag tctggaatta tatatgttgc aactagaaag gaagttgaaa
     6721 atatatataa tggtttatct aagagaaata tatctgtatc aaaatatcat gcaggtttat
     6781 ctaaaaatga aagaagtaaa aatcaagaag attttataaa tgataatgtg gatataatgg
     6841 tagcaacaaa tgcatttgga atgggtattg ataaacctaa tataagatgg gttatacatt
     6901 acaatatgcc tcaaagtata gaaaactact atcaagaaat aggtagagct ggtagggatg
     6961 gagaaaaaag tgagtgtgta cttttatttt ctccaggaga tgtacatata caaaaatatt
     7021 taattgatat tggagttgaa aatcctgaaa gaaaattatt tcagcataag aagcttcaat
     7081 acatgattga cttagtttac agtaattcat gctatagaaa gacaatattg agttattttg
     7141 gagaaaatta tatagacaat tgcaataatt gtagcaattg tttagtagag ggtgaaatag
     7201 tagataaaac tatagatgca caaaaagtca tttcatgtgt agctagaatg aaaagaagtt
     7261 atggtgttac tactattgta gatgtactta gaggttctaa gaataaaaag attttacagc
     7321 ttggattaaa tactttgtct acatataaca ttatgagaga ttattctagt gaagacttaa
     7381 agaattttgt aaatacattg gtttctcatg gttttttaga tttagttgag actctaggca
     7441 atggaaatag aggtagtttt cctactataa gattaaatga gatgtctatg aaagttttaa
     7501 aaggtgaagt gaaagtagag tttaaagaaa ttgttatgac aaaatcattg gaagtagagg
     7561 atgagttata taatgcttta agagaactta gacattcaat tgcaagtgaa gaaaggatag
     7621 ctccatatat ggtatttgga gatggtacac ttagagccat gtctagtagt tatccaatta
     7681 ataaagaaga aatgctagat atctctggag taggagaaat taaatatcaa aaatatggta
     7741 gagattttga agcagtaatt aaagaatatg tcgaaaaaaa taatataaat aaaaaaatag
     7801 atgaaaaaaa tgtcgattta aagaacgtta atagtgattt cttagaggtt aatacagata
     7861 aagttttata tgaaaaactg ttagtcatta gagaagaata tgcaacaaag gaaaaattac
     7921 ctgcatatat gattttagcc aataaggctt tgaaagaaat aagtggaaga tatccacttg
     7981 atgaagaaca attaaaagat atttctggca taggtggagt aaaatttgaa aaatatggag
     8041 ctaatatttt ggcagaggtt aataaatatg taaaagaaaa taatattaat gttatttggg
     8101 agaaaaaggg caaaaggaag ttaatattag atggagaaag tagaaaaaat aatgaaatag
     8161 cattagattt gctaaatcaa ggaaaagata tacaaagtat cagtcaagaa atagaagttt
     8221 cattgtcaac cattattgga tatgtgcatg actatgtaaa ggatggacat agtatatcat
     8281 ttgaattagg actagaaaat ttttataatg aagataaaaa gaagatgatt ttagaagttt
     8341 gcgaaaaagt tggctatgat aatttaaata acataaaaag aaaacttcca gattcaataa
     8401 agtatgaaaa tataagagct gtgatgcttg actcttatct tgaaaactta aaaaataata
     8461 ttatagcaga gtaattttta acttaataaa aataaaattt gtattattgt gttgaactaa
     8521 taattttgct gtgttatact gaatttgtaa aaataatgaa aaattagagc taggggcgct
     8581 agaattggct agctgagaga taaagccgta acttacaaac cctaataacc tgatcaagat
     8641 aatgcttgcg aagggaagct ttttaatata attttattat ataaataggc aaattattca
     8701 atgttaggag aaatttgtct aagtgtatat acgattataa gagtgctttc caaacgagga
     8761 aaagcacttt ttttattttc aaaaactata actttaatct tggaggattg taatatgaac
     8821 tatacaacac aaatggatgc tgctagaaaa ggcataataa ctaaagaaat ggaaatcgtt
     8881 tctcaaaaag aacaggtaga tgtaaatgaa ttaagagaat taatagcaaa tggacaagtt
     8941 gttataccag ctaataaaaa tcataaatct ttaagtgcag aaggtgtagg taaaaattta
     9001 agaacaaaaa taaatgtaaa tctaggaatt tcaagagatt gtaaggatat agaaaaagaa
     9061 cttgaaaaag ttagagtagc aatagacatg aaagctgaag ctataatgga tttaagtaat
     9121 tatggcaaaa ctagagaatt tagagaaaag gttgttgaaa tgtcacctgc aatgataggt
     9181 tcagtaccta tgtatgatgc agtaggatat ttggaaaaag aattaaaaga tataacagaa
     9241 gaagagtttt taaatgtaat aagacaacat gctattgatg gagtagactt tattactata
     9301 catgcaggtc ttacaagaag tgtttgccaa aaaataaaaa atcatgagag attgactcat
     9361 atagtatctc gtggaggttc tttattgttt gcatggatgg aattaaataa taaggaaaat
     9421 cctatttata ctaattttga taaaatattg gatatatgtg aagaatatga tgttacattg
     9481 agtttaggag atgcttgtag accaggttgt ataaaagatt ctactgatgg tgttcaaatt
     9541 caagaacttg tagtattagg tgaacttact aaaagagcat gggaaagaaa tgtacaagta
     9601 atgatagaag gtcctggaca catggctatt gatgaaatag aagcaaatgt agtattagaa
     9661 aagagattat gccatggagc acctttttat gttcttggac cattggttac agatatagca
     9721 ccaggttatg accatataac aagtgcaata ggaggagctt tagcatgtgc taaaggagta
     9781 gattttttat gttatgtaac tccagcagaa catttaagat tgcctaattt agatgatatg
     9841 aaagagggta taatagctgc aaagatagct gctcatgcag gagatatagc taaaaatgtt
     9901 aaaggtgctc gtgaatggga taataagatg agtaaagcta gagctgattt agactggtgt
     9961 gagatgttta gacttgcaat agaccctgaa aaagctaaga gatacagaga tgaatcaact
    10021 cctactcatg aggatagttg tactatgtgt ggaaaaatgt gctcaatgag aacagttaag
    10081 aaaatattaa acaatgagga acttaatcta atataatttt tggctagtat gactggaata
    10141 aaaagaagct gaaaagagga ggtgatttag tgaaggttaa tgggaaggaa attgaatttg
    10201 aaaaagattt gactgttata gatttattaa ataaatataa tttaaaaagt gatagagttg
    10261 tagttgaagt gaatttagag ataattgaag aatctaacta caatacatat gtattaaaag
    10321 acgaggacat tgtagagctt attagcttta ttggaggtgg ttaaaataaa aatatttgta
    10381 aatgaattat taacagatgt tgaggaaaat acaactgctt ataaagttag agatagctat
    10441 aacaataaat gtgatgtatt ggtgttaaat ggatatccaa ttaaaacaga tatgcccctt
    10501 tgtgaaaatg ataaattaac acttatacaa aaaggagtta agccatcact tgatgaattg
    10561 gaaagcttgt tgattgctag acatactcca aatatacata ataaattaaa aaaagggaaa
    10621 gttgctatat tgggattggg gggacttggt tccaatatag ctatctcttt agctagaata
    10681 ggagtgggag aacttttact tatagattat gatgttgtag aaccatctaa tttaaataga
    10741 caacagtatt ttatagatga tataggaaaa ctaaaaactg atgccatgat agaaaatata
    10801 aaaaaaataa atccatttat aaaattaaat aaacgagata tttttttaaa caagaacaat
    10861 atggatacta ttaaggattc agatttaata attgaggcct ttgatgatgc ttcatgtaaa
    10921 gcacaggtat gtaattatgt attaataaat ttaaaagata aatatttaat agcatcatct
    10981 ggtatggcag gttactatga ttcaaatata ataactacta agaagataaa agacaggttt
    11041 tatatatgtg gggattttgt gaatgaagct aaatttggtg aagggcttat ggctccaagg
    11101 gttgccatct gcgcaaatca tatggcaaat ttggcaacgc aaatacttat agatatgtaa
    11161 atattaaaat tagatataaa aaagattgaa tattaaaaaa atttaacgtg gagggaaata
    11221 atatggataa attggtactt ggtggacatg aatttaatag cagattattg gttggaacag
    11281 gtaaatatgg ttcaaataat atattgcctg aggtaataaa agaaagtgga agtgaaataa
    11341 taacaatggc acttagaaga gtggatttag acaataaaca agaaaacata ttaacgtata
    11401 ttccaaaaga gatgacaata cttccaaata catcaggagc tactaatgca gaagaagctg
    11461 taagaatagc tagaatatct agaaaaatgg gatgtggaga ttttataaaa atagaagtaa
    11521 tatcagatac tagatattta cttcctgata atgaagaaac tataaaagct acaaagatac
    11581 ttgctgatga ggggtttata gttcttccat atatgacacc agacctttat gctggaagaa
    11641 gacttataga agctaatgca gccgcagtta tgccacttgg agctccaatt ggttcaaata
    11701 gaggtcttca aatgaaagaa atgataagaa taatgataga tgagttggat ataccaataa
    11761 tagtagatgc aggtattgga aaaccatctc aagctatgga ggctatggaa atgggagctg
    11821 atgcagtact tgtcaacact gctatagctt cagcaggaga ccctgtacaa atggctagag
    11881 catttaaatt agctgtagaa ggtggaagag aagcatatat tgcaaaaaca ggaaatgtgt
    11941 cagaatttgc aaatgcatct tctcctttaa caggtttttt aggcaattta tagaaagtgg
    12001 tgattggtga tgagttttta tgatgtaata gaaaaatata gggattttga ttttgatgga
    12061 tatttaaata atgttactga taatgatgtt ttaagaagtt tatcaaaaga caagcttgaa
    12121 gattttgata tattaaattt gctttctaaa actgcagtaa aacatttaga agatatggca
    12181 caaaaagcac ataaattaag tgttcaatac tttggtaaaa cagtatgttt atatacacca
    12241 atgtatatag caaattattg tgttaatcaa tgtgtttatt gtagctataa tataaagagt
    12301 ggaataaaaa gaaaaaaatt aactatggat gaaattagag aagagggaga agcaatttca
    12361 aaagaaggtt tcaaacatct acttgttttg actggagaaa gtagttttca ctcaagtgta
    12421 gaatatatag gtgaggctat agaaatatta agagagaaat ttccctctat aggtatagaa
    12481 gtttatccaa tggaagtaga agaatataag tacattgtag ataaaggtgt tgaaggatta
    12541 acagtatatc aagaaactta tgacgaggaa atatataaga gagtacatat aaaaggccca
    12601 aaatcaaatt atgaatttag actagatgct cctgaaagag gggcaaaagc aggtatgagg
    12661 actttatcaa taggtgcact tttaggtcta aatgatttta gaaaagaaac tttttttaca
    12721 atattgcatg ggaaatactt aaaaacgaaa tatcctcata ttgagctttc atattcaaca
    12781 ccaagaatga gaccttttaa agggtgtttt gaggaattgg tagatataag tgatactgat
    12841 ttagttcaag ctatggtatg tatgagatta tttgaccctc atgcagctat aaatatatcc
    12901 actagagaaa atttagaaat gagaagtcat ataatacctt taggagtaac taaattgagc
    12961 gcaggtgtat ctactgatgt aggaggtcat tctcaagatg aacatgatac tgctcaattt
    13021 aagataaatg atgaaagtac tgttaaagat gtggagaaaa tgctaaactc aattggttat
    13081 cagcatgtgt ttaaagattg ggaaagattt taaatgtatc tgataacaaa cagaaaatta
    13141 tgtagtgagg aacgatattt agaagttatt aaagagtcta tattgagtgg tgtagaaaat
    13201 ataataataa gagaaaagga tttggaatat caagagctaa gaaaattata tatgaaaata
    13261 aaaactaaaa tcaattgtat agattttcaa gagcaaatat cagatgaaag tttgaaaact
    13321 aatataaatc aaaaagagtg tagaaataaa ttcaaggtta attttataat aaatagtaac
    13381 attgaatttt ttgaaaaagt ggattgtcaa ggtatccatc ttccttttaa attattcttg
    13441 aatttaattg aaaataagta taattttaat gaaaataaaa tactaggatt atctttacat
    13501 aaagtagagg aagtagatta tttagaaaaa ttaataagaa atcaaaatat aaaaatagat
    13561 tacataacat tatcacatat atatgaaact aaatgtaaag aaggattaaa tccaaaagga
    13621 attgagcttt taaaagaagc taaaaagatt actgatatta aaataattgc acttggggga
    13681 attttaccat ctaatgtcaa agaaacatta aaatactgcg atgattttgc tataatgtca
    13741 actataatga ggtctaaaga tattaaaaaa accatatcaa attataatga aaagttgaat
    13801 tagtaaatat agtatattga atttttaact taaataaaaa taaaaaagtt atcttttaaa
    13861 ttttaaaagg tagctttttt attttatatc tttgctaata aaaaattgta aaaagggaaa
    13921 actttagtca gagagattaa acaatatcct agaaagattt aaaatttagt aaagtatgga
    13981 ggacattata atgactgaca gtaaaaagaa acgatttatt ataccacaca cttttacaat
    14041 aatattttta ctaatagtct tgatggcaat attgacttgg atagtaccaa gtggtgaatt
    14101 tagtagagaa gatgtagaag gtagaatggt agttgtacca gggacttatc aaagtgtaga
    14161 aagtaatcct caaggatttg cagatgtttt taaagcacca atagaaggct ttatagattc
    14221 agctgaagtt gtaggttttg ttttaatagt tggaggtgct tttggtattg taaataggac
    14281 tggagctata gaagcaggaa tagcttcggt tatctctaag tttaagggaa aagaacttct
    14341 tataattcca atatcaatga ttttatttgg acttggaggt acaacatttg ggatggcaga
    14401 agagacatta ccattttata tgatatttgt tccactcatg acatccttgg gatatgattc
    14461 tctaacagca atagcaattg tatttatagg agcagcagca ggagccgccg cttcaacaat
    14521 aaatccattt tcagttggta ttgcacaggc actagctcaa cttgctcctg gttcaggagt
    14581 aacttacaga gtagttattt ttatagcata tatactaata agtatagcat ttgttatgta
    14641 ctatgctaag aaggtaaagg aaaatcctga aagttcaata gtatatgaaa tagataaaaa
    14701 taataaatca atacttaatc aaacttcaac agaaataaaa gaatttactt ttaaagaagt
    14761 atctgttctt gctatatttg gaatagggat ggcaataatg atttggggtg ttttaagtca
    14821 aggatggtac attccagaaa tatcaatgtg ttttgctgca ataggtgtgc tttctggatt
    14881 ggttggtaga ttatctcaaa ttgaaatctc agaaagtttt atagatggag caaaagattt
    14941 agcatctgca gctcttgcta tagctttggc tcgtggaatc gtaataattg ctcaaaatgg
    15001 atttataata gatactatat taaattccgc agcagccttt ctaggtgggc ttccaaaggt
    15061 tatatttgta tatttatcat ttttcttgga agctgcattg gcatttttaa ttccatcatc
    15121 ttcaggtctt gcttcactta cagtacctgt acttgctcct cttggtgatt tggttaatat
    15181 atcggctcaa gtgatagtaa ccgcttatca atttggtgtg ggtcttacaa actttataac
    15241 tccaacttca ggagtactga tgggagcact tgcagtagcc catattccat tttcaaaatg
    15301 ggtaagattt ataatgccta tcttgttgat tttgacagct gtatctctag tctttttaac
    15361 aataggtatt tatatagggt tttaattaag tgtagtttta tttaagatat ttagtaatgg
    15421 tataaaatga taatatgttt tcaataaatt gccactaatt taattagtgg taatttattt
    15481 tttgtattta aaaataaatg tatttacttg tattttgaat taatatctac ttagacattg
    15541 ttttaattgt tatattttca tttaattaag cattgattta tcgtttttta tatttcatat
    15601 aacaatgcaa aaaataattt ttatatagta aatgacaaaa gtttacaata aatgtatatt
    15661 ttcttactca ttccttaaaa ttactcattt agtttaataa ctctcatttc atatgtaaag
    15721 ttggtaaaaa aattaaaatt aaaagtaaaa aactaaataa attaatgtta atttgaaata
    15781 tatatgaagg tttttggaga atacttactt atgataggaa aaaatgttaa aataatagtg
    15841 taatcaaata taaaggactg aaataattaa cagttattaa atattaggag tgtaaaaatt
    15901 tatgggatta aaagaatttg attttattga agagtctatt gatatgttga ggacaatgtc
    15961 accaacatta gaaactatat cagatgaaat agaagagtat tttgaaaata tcttggatga
    16021 aaagaatcaa gaatatataa atgtaacctc tagaataaaa tcagaatcaa gtttaagaga
    16081 aaagataatt agaaatagat atttaaaaaa atatggagaa gcaagtaatc ttattcataa
    16141 tgtttctgac ttaataggcc taagaattga gtgtagattt attgaggatg aaaacaaaat
    16201 atatagactt ttgagaagat attttaataa aacagatgac aaaataaact attataataa
    16261 agagaataag aatattaaat taaaactatc tgagaggcaa cccaataagc aaaaaaatgg
    16321 atttgaaata tataaaatag atggagtttt tgcgtattta gatagagaag taaaatttga
    16381 acttcaaatt aaatctcttg taaatgtgtt ttggagtgaa atagaacata aaataattta
    16441 taaaaataat acatatttac ttgctgataa atttataaag gatatgatgg actctataaa
    16501 gaataatctt actatgatag ataatcagct tttaagtatt tataaaaact ttcattcagg
    16561 gaagtctttt aatatgaaag taagtaaaaa agaaattgaa aaattatttg cgaaactagt
    16621 ctatgacgct ttttctgaaa agatgaataa aagtataggt tttgttgtag attttaaaaa
    16681 accatgtgaa actatattga actattcttt taataaacaa gatataagtg atgaagtttt
    16741 aggtaatttt atgcttgatg agtttgcaag attaaatgaa attgtaaaca aggatataga
    16801 ttttaatgag caaatagaat ttgaaagaga accaacattt gatgataagt tttgtaaaga
    16861 cctaggaaat cattttagaa gtagattaaa cacagaattt ccatggaacc tattttttag
    16921 aatattattt gaaatagagc catataacaa taccaaagac tttgaaaact ttgtagagtt
    16981 tataaaagaa aatgtgctac tagaagaaaa tagggagcaa ttattatgcc aatttgaaga
    17041 agatagcaag cttataattg aagatatata tgattgtata tttaatagta taagtgagat
    17101 tgatagtgtt gaaattttat ataactataa tcttgaaaaa ataaacttta catcaagtga
    17161 aatcataaat tgtatatgta gagaatatga gtgttatggt gaatacttag aagaaaaaga
    17221 aaagcttatg aatacattta aagaaaagat tattcaaatc tttgagtaat aggtcaatgc
    17281 tgaagtggcg ctaatgcgcc atttttcaaa tatatatgaa ttaagtaaaa ttattttgga
    17341 ggaggcgatt tgtatagaga aaacaggtgt aattttggca gggggaaaaa attctagaat
    17401 gggtagagat aaagcctttt tagagttaca tgataagctt tttattgaaa ttgcaataga
    17461 agcatttaaa aactttgatg agttgataat aatatctaat aatgaagaat tatattctaa
    17521 atacgatatt aaagtttata atgatatagt aaaagatgtt ggtccaattg gaggaatata
    17581 tactgctctt gaatatgcaa agtatgatat agttacaatt gcatgtgata tgccatatct
    17641 aaataatcaa atagtcgaaa gaatagctaa taaaatgaat gataaaagtg tgatttcagt
    17701 gacaaatgga aagttacaac cattgtgttc tggatataaa aagagtataa taaataaagt
    17761 atctttatgt ataagggaaa atgatttaaa gctcagaagc tttatagata aaatagataa
    17821 aagttatata tactttgatg aagaagattt atttttaaat gtaaacactg ttgatgagta
    17881 tgaaaaatta actaaagttt aattagctat ggttgtaaaa tagagtagga tttatatgga
    17941 ggagagttta tgaatttact taaatgcgat tcttgggcag taatattaaa taatagcgat
    18001 aaagaaagta aagcatataa aatattagat gaacttaaaa ggaatatgta caaagttgtt
    18061 gctatagatg aagagaaaaa acctattgaa ggaatagatg tgtatgaatg tttaaaagat
    18121 gttccacaca atattgatgt tgtagctatt attgataaac aatctaagat ggatgtgatt
    18181 ttagaagaag tagagctttt ggatatacaa aatatgtggt ttgaaaaagg aagctttagt
    18241 gaaatgatga ttaaaaagac taaagattta aaattaaata tagaatataa tttaagttta
    18301 tatgatgagt tgactagata aaattatatt tcttaataat aaaaatataa ttagttaata
    18361 aaacatatta ttttatagta tagattggtt aataaaagta cagattggtt aatgaaacat
    18421 taaattacaa tatttgttaa aagaattgtg agttacatga tataatgtat taaataggat
    18481 actaaggagt tgatatatta tgttaatagt aactacagaa aaagtagaag gtaaaaagat
    18541 atcaaaggtt ttaggattag tgagaggaag tacaataaga gcaaaacatg ttggaaaaga
    18601 tataggagca agttttaaga atcttgttgg aggagaactt actggatata atgaaatgct
    18661 cactgaagca agacaaattg ccataggtag aatggttgaa gatgcagaag ctaaaggtgc
    18721 aaacgcagta atagcattta gactgtcttc agcttcagtt atgcaagggg cagcagaaat
    18781 gcttgcttat ggaacagcag ttgttttaga agatgataat agtattcttg aaaaataaaa
    18841 tataaatagt taaaaagagg catcttttaa aaattttatg gatgcctttt tttatttgaa
    18901 aaaactaatt tgagaagaaa ttttaactat tatgacaata aaattagggt gatattatat
    18961 aaataagtaa aattatatta tataatgtat ggtatagact ttaaaaattt tatttgtaga
    19021 gaaaaatatg gacaatgtta taataattat agaaattaaa agtacttaca ttcattatgt
    19081 atgtgacatg tataattagt tttatattta atacagtttt gaagtaatat cttcattttg
    19141 agaacgttgg aataataaaa aatgtatgta ttataggtga tataaataaa aaataaatat
    19201 aatatgttat attgctgata gaaaagagaa taaaagaaaa taggttttat taaaagagat
    19261 ttttatttat aggacttaat caatgtatag aggggtgaga acatatgttt aatgttgcaa
    19321 ttataacact tagtgataaa ggatatgaag gaaaaagaga agatataaca ggaaaaaagt
    19381 taactgaatt tgttgaaaat acaggagctt ataaagttac tgagtatgta cttataaaag
    19441 atgataagga aatgctaaag gaaaatatta taagactttg taatagtaat aaaatagatt
    19501 taattcttac aaatggaggt actggttttt caaaaagaga tataacccca gaagcaacta
    19561 aggaagtgtt ggaaaaagaa ataccaggtc tgagcgaata tatgagaatg aaatccacag
    19621 aaattactaa aaaggccata ctaagtaggg gtgtgagtgg aataagaaat aattctataa
    19681 taataaattt acctggaagt cctaaaggtg cagttgaaaa tctaagtttt ataattgatg
    19741 ttttagacca tggcatagaa gttttaagag gagaagctac agaatgtgcc actaaaaaaa
    19801 gtgaatagta tataatctag atagataaaa aatagacaaa gaggtagata aagtatgatt
    19861 gataagtatg gaagaaaaat agactacctt cgtatttcac ttacagataa gtgtaattta
    19921 aggtgtgtct attgtatgcc accagatgta aaatttgata aaaattatat aaatgaaaat
    19981 ctaagctttg aagactacaa gtttattata aaggctatgg ctgaacaagg tataacaaaa
    20041 attagattta ctggaggaga acctctttta tatcctaaat taaatgagct tataaaattt
    20101 acaaaagaag aatgtggaat aaataacata ggaatgacta ctaatgctat tgggctttct
    20161 gatagaattt ttgagcttga aaaatgtggc ttaaataaag ttaatataag tttagattct
    20221 ttgaaggaat ataagtataa atccgtaaca agaggaggaa acttaaaaga tgtcttaaaa
    20281 tcgattgaat cttgtttaaa cttaggtata aaagtaaagt taaattgcgt tgctataagt
    20341 gggtttaatg atgatgagct taaagatttt atgtttttaa cagttaaatc accaattgat
    20401 gtaagattta tagagcttat gccattaggt gaggctaata gagtttataa gaagggttat
    20461 tttaatgtaa aagaaatgat tgaaaaaata tatggtttat ataaagtcaa aaatgatgaa
    20521 aaaagtacag cagaatatta tatgtttaaa ggagctaaag gaagaatagg aattataact
    20581 ccattaagct gttcattttg taacacttgt aatcgtatta gacttacttc tagtggtgca
    20641 ataaaattat gtctacattc aaaagaagaa atagatataa aaccattttt acataaacca
    20701 ttgttattta gagaatctat gaaagatata ataaaacaaa aaccagaaaa acatcattta
    20761 atagaaaata aatgtagtga tacaaatagg cgtatgtatg aaattggagg ataagtaatg
    20821 ttaacacata taaatgaaaa agggtatgcc aagatggtgg acataagtga aaaagatgat
    20881 actaaaagaa cggccgtagc tacagctaca attactctta gtaaggaagc attagaaaaa
    20941 gtaaaaaatg gtcagataaa aaaaggagac gtattgtctg tagctcaagt tgcaggtata
    21001 atgggagcta aaaatacatc ttcaaacata ccaatgtgcc atcagattaa attagttgga
    21061 tgtgatttag aatttgatgt agatgattat atgtctgcta ttaagatata cgcaacctgt
    21121 aaatctcttg ggaaaactgg agtagaaatg gaagctttaa atgcatgttc tttagcagca
    21181 cttacaattt atgatatgtg taaagcagtt gataaaaaaa tggttataag taatatccat
    21241 cttgtggaga agactggtgg aaaatcagga gattttaagt tcgagtaaat taataatata
    21301 ttgggttaaa atttggagga aatatggcga aggtagtgtc aataaatata agtgagaaaa
    21361 agggtgtaat taaagttcct gtaccatgtg cagagctaaa aataaatcat gggatagttg
    21421 gagatgcaca tgcaggaaac tggcatcgtc agataagctt acttggaaaa gaaagcatag
    21481 ataaaatgaa aatgaaaggc tttgaccaat taaaatatgg agattttgct gaaaatgtaa
    21541 ctacagaagg gatagaagtt ttttccctgc ctgttggtac aaagttaaga atagggcaat
    21601 gtgaagttga agtaactcaa atagggaaaa aatgtcacaa tggatgtgaa attaagaaat
    21661 tgacaggtga ctgtgtgatg ccacgagaag gaatttttgt aaaggttcta aaagaaggaa
    21721 ttataaatgt gaatgattca atagatgtaa tctaatgaag tcactattaa aatataatta
    21781 taagtaaata ctattaagat ttaaatatat attttaagag agaattaatt taaataaaat
    21841 attttagtta taaa
//
LOCUS       DAGQWR010000070        21551 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.070, whole genome shotgun sequence.
ACCESSION   DAGQWR010000070 DAGQWR010000000
VERSION     DAGQWR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.070"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            211..1011
                     /locus_tag="MIW95_002204"
     CDS             211..1011
                     /locus_tag="MIW95_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBY3151529.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            1538..3778
                     /locus_tag="MIW95_002205"
     CDS             1538..3778
                     /locus_tag="MIW95_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBY3151530.1"
                     /translation="MSNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNDIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSRDGEISEATKKKLSKLLIEDRCPICDGRRLNEGVYRSLI
                     NGYNIADLTSMQIDELAEVIKSINEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHVVDVGPKAGKYGGKVVYEGSYKNLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEIKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQRKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            4192..5727
                     /locus_tag="MIW95_002206"
     CDS             4192..5727
                     /locus_tag="MIW95_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBY3151531.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTAMTISGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGIGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNISDLKNVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVIVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSEVFKMVGDILTDKEMAKMNYDVENNNLEPREVACEFLNKK
                     GLL"
     gene            5743..6504
                     /locus_tag="MIW95_002207"
     CDS             5743..6504
                     /locus_tag="MIW95_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151532.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            6842..7710
                     /locus_tag="MIW95_002208"
                     /pseudo
     CDS             6842..7710
                     /locus_tag="MIW95_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            7904..8155
                     /locus_tag="MIW95_002209"
     CDS             7904..8155
                     /locus_tag="MIW95_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBY3151533.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            8267..9046
                     /locus_tag="MIW95_002210"
     CDS             8267..9046
                     /locus_tag="MIW95_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY3151534.1"
                     /translation="MNNTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(9181..9831)
                     /locus_tag="MIW95_002211"
     CDS             complement(9181..9831)
                     /locus_tag="MIW95_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151535.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIDHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(9828..10331)
                     /locus_tag="MIW95_002212"
     CDS             complement(9828..10331)
                     /locus_tag="MIW95_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY3151536.1"
                     /translation="MKLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGRFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(10380..10895)
                     /locus_tag="MIW95_002213"
     CDS             complement(10380..10895)
                     /locus_tag="MIW95_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBY3151537.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKIDVEENHCIDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            11211..11702
                     /locus_tag="MIW95_002214"
     CDS             11211..11702
                     /locus_tag="MIW95_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY3151538.1"
                     /translation="MRTIDIKGENMRELLKWVSIINRYNTNYIDRKLADIGINNSQYF
                     YVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVKDKRTWHLYPTE
                     KSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMNEEAKLEAKENG
                     RKI"
     gene            11686..13089
                     /locus_tag="MIW95_002215"
     CDS             11686..13089
                     /locus_tag="MIW95_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY3151539.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(14031..14624)
                     /locus_tag="MIW95_002216"
     CDS             complement(14031..14624)
                     /locus_tag="MIW95_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY3151540.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
     gene            15185..15415
                     /locus_tag="MIW95_002217"
     CDS             15185..15415
                     /locus_tag="MIW95_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151541.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            15472..16122
                     /locus_tag="MIW95_002218"
     CDS             15472..16122
                     /locus_tag="MIW95_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBY3151542.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRMYFNRKNRIVTK"
     gene            16336..17007
                     /locus_tag="MIW95_002219"
     CDS             16336..17007
                     /locus_tag="MIW95_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3151543.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCITANDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            17004..18134
                     /locus_tag="MIW95_002220"
     CDS             17004..18134
                     /locus_tag="MIW95_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3151544.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDAISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLIINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RKGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            18539..19384
                     /locus_tag="MIW95_002221"
     CDS             18539..19384
                     /locus_tag="MIW95_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151545.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            19384..20412
                     /locus_tag="MIW95_002222"
     CDS             19384..20412
                     /locus_tag="MIW95_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151546.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGVKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            20415..21428
                     /locus_tag="MIW95_002223"
     CDS             20415..21428
                     /locus_tag="MIW95_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151547.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTML
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGAPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILVVHCVVWFMIYIILDKKRKLS
                     FSR"
BASE COUNT         8096 a         2289 c         3680 g         7486 t
ORIGIN      
        1 caatgttgca gttagaaggc ttttttattt tattatagtt cgtgtcaaag attgtatgaa
       61 acaataaaat attgtaaaat aatttgtata tttagacctg attgacacaa aggtttataa
      121 gatatttgag ttgattatac gaaattctat gaagtagtaa ccctgtgact ttaatcatgg
      181 gaggttcaga agaaggagaa aattttaaat atggaaagta aaacgaagta cataggaata
      241 agtatattaa tattattgat gatattttta attgcaaaga attttaacgg aaaacttgaa
      301 atacctattc ctgagagtaa tagtagaaaa tttaaatcag cagcagtgtt ttatcctcaa
      361 catcaagatg atgaggtttt atggggagga agtgccattg tagatgctat aaaacagtgt
      421 ggtgttgata atgtatatgt ggtattagta tcagatggtt ctggagttaa cgttttcaaa
      481 gctaacacaa aatttagaaa cttaactagg aagcaaaagg aagagcttag aaataatgag
      541 tttaaatcag ctttgagaga gcttggagtg aaacctcaaa atgtgattat acttgcagat
      601 atagataaaa aggaaggcac acattatgaa ttaatggaaa agactatatt ggattttgaa
      661 cataagttta aaggcaatgt gacccatata gcccatcact ataagtatga tgaccatata
      721 atgcatagaa agaatgggga agttttaaag aaacttaaac gtgaaggtaa gataaaagat
      781 gacttgtatt ttgttaagcc aaaatatgta aagtatatac caagaaatga aagagctatc
      841 tataaagcaa tggattttga tgactacaat aaggttaaga gtgcttgtta tgaatataag
      901 gttgtaaatt caaagcgtaa aatgtatggt ataggatata tttcggcaca tagttatttt
      961 gaacatcttc taaatgaccc tgaacttacg tctgtattaa gtgaagatta atttcttata
     1021 agtagtaagt tatgtttgat aagttgattg gaattttcta tagttttgaa ggtattggaa
     1081 atttagtttt atttaaagat ttaaagatag aggtttttaa tgtttaaaat tttaaagtta
     1141 agttgaataa ttgtatagaa ttatttgtga aagaagaaac agtgtaaaac atagtatctt
     1201 atttatggtt ttaaaatcat aggcaagata ctttttttat gtaactaata ccattttgag
     1261 gttattaact taagaatttt atataattta acactcatat ataatgatga tatttaggta
     1321 gaatagatgc aattacgctt aaataaaata actgtatttt ttataaaaat caaaccctac
     1381 actatactac cagataaaat ctacatgcta taatttgacc tgtgtttata aaaaaaggag
     1441 taatctaata aaatagtatt tcacttatgt ataactaaca actattatta cgattttctc
     1501 taaataattt aaagaaaaat aatgcaggag gtaaaatatg agcaatgaat ttataatttt
     1561 aaaaggttgc aaagagaata atttgaacaa tatttcctta aaaataccaa aaagaaaaat
     1621 aaccatattc acaggggtgt cgggttctgg aaagtcatct attgtatttg agacaattgc
     1681 aaaagaatca caacgccaac taaatgaaag gtttagtaca tttgtaagaa gttttttacc
     1741 taagtatgga gaggttaaag ctgattgtat agaaaatctg tcaacaccaa ttatcataga
     1801 ccaaagtaga cttggtggaa attcaaggtc aaccctaggt actattacag atgtaaattc
     1861 ctttttaaga gctttatatt caaggtttgg tagtgagtat ataggtaatg ctaatatgtt
     1921 ttcttttaat gatattgatg gtatgtgccc acactgtcat gggcttggaa aaaagctagt
     1981 tcctaatatg aaccaaattc ttgatatgaa taaatcatta aatgaaggtg ctatattgct
     2041 ttcagggttt ggagtgggtt cttggcattg gaatatattc gctgaatcag gattttttga
     2101 tattaataag aggatatgtg attattcaga agtagaattg gaaaagtttt tgcatggtaa
     2161 agcagaaaag attaagatag aaaatgctgg tcaaactaac atgacatatg agggattaat
     2221 ggttaaattt aatcgtctat acttaagtag agatggagaa atatctgagg ctactaagaa
     2281 aaaattgagt aaacttctta tagaagatag atgtcctata tgtgatggaa gaagacttaa
     2341 tgaaggagta tataggtctt taattaatgg ttataatata gcagatttaa ctagtatgca
     2401 aattgatgaa ttggctgaag ttattaaaag tataaatgag ccagaagcac aacctttaat
     2461 taaaggaata atagaaaagt taaacagtat catagatata ggtcttggat atttaacact
     2521 ggatagagag acatcttctc tatcaggagg ggaatctcag cgtataaaga tggttaagta
     2581 tttaaatagt aatcttgttg atttaatgta tatttttgat gaaccaagtg taggacttca
     2641 tccaaaagat gtgtataaat taaataatct tttaaaaaaa ttgcgtgata aagggaatac
     2701 aattatagtt gttgaacatg acccagatgt tattaagatt gcagaccatg ttgtagatgt
     2761 aggaccaaag gcaggtaagt atggtggaaa agttgtgtat gaaggcagtt ataaaaatct
     2821 acttacaagt ggtactttga cagggaatgc tttaagtaaa tttcttccta ttaaagaaag
     2881 tgtaagagaa catagtggat atttagaaat taaaaattgt aataaaaaca atttaaaaaa
     2941 tgtatctatt aagataccta aaggtgtatt gactctagtt actggagtag caggagcagg
     3001 taaaagtacg ttgattaagg atgaattttt aaagcaaaat cctagtgctg tacttataga
     3061 ccaaagccct gtttcagcaa attcaaggtc gagtttggca acatatagtg gaattatgaa
     3121 caacataaga agcatatttt caaaaacaaa tggcgtaaat gcttctttat tcagttcaaa
     3181 ttcagaagga gcttgtgata actgtaaggg ttctggaata gtagaaatga atcttgcttt
     3241 tatggagagt ataaagagta cctgcaatgt ttgtgaaggt aaaaaattta aaaaagaagt
     3301 tttagaatat aggtttcaaa ggaagaatat aattgaagtt cttgaaatgt ctgttttaga
     3361 cgcaattgag ttttttaatt taaaacagat taaaacgaaa ttacaatgta ttgaaaaaat
     3421 gggtattgga taccttactt taggacaaac tcttgatact ttgtctggag gagagtgtca
     3481 aagattaaag cttgctagtg aattaaataa ggaaagttca gtgtatatat tagatgaacc
     3541 aacaacaggt cttcatatgg ctgatattga gaattttata agtataattg aagagattgt
     3601 agataatgga aatacggtca taataattga acacaatata gatgtaatta aaagagctga
     3661 ttggattatt gagctgggac ctgaaggagg gacaaaaggc gggagagtaa tatttgaagg
     3721 tatcccaaaa caattatgta acaataaaat atcattaaca gcaaaatata tagtataata
     3781 aataaaatat aataaaatta taatgatatt ttaataaata taatgggtat ataaaatgat
     3841 aattaaactt aacagtaata aataatgtga gaaatattca aatttaatta aataaagtgt
     3901 cagaaatttg tttaaatttt agtattttaa cgattctgac cttttttctt atttttatgg
     3961 agagtatcga agatggagtc tctaatagtt acttttttgt gtctacttgc aagtttgatt
     4021 aaaaaatggc tatacacact tgtgacttca gtcatgggag gttcagatta taatcttaat
     4081 atataatttg tgaaataatt tgatgacgat ttgctgatgt ataaacaata aagattaact
     4141 acattaaaat ttaaatcttg ttaagtaaaa cttataaaag gagagagatg gatgataaat
     4201 gatattctaa ccttattatc tgaaagaagt gattttttta taaagctatc catagaacat
     4261 ttgatgattt ctattatttc tataataatt gcgattgttt tgggtggtat aattggtatt
     4321 attgtaagtg aatttcataa ttcttctaaa cctatattgg gaactatcaa ttttctatac
     4381 accattccat ctatttctat gctaggattt ctgataccat tttccggaat tggaaatact
     4441 actgctgtta ttgcacttac catttatgca ttattgccaa tggttagaaa tacatatact
     4501 ggaattacta atgtggataa aaatattctt gaagcagcta ggggaatggg cagtacagat
     4561 tttcagatat tgtataaaat aaaacttcca ttagccctac ctgttattat gtctggtatt
     4621 agaaatatgg tgattatgac aattgcatta gctggtatcg cttcatttat aggtgctgga
     4681 ggcctaggtg tagccatata tagaggaatc acgacaaata acacagcaat gacaattagt
     4741 ggaagtatac ttattgctct attagcttta atatttgatt ttattttagg gctggtagaa
     4801 aaattttttc aacgaaaaag aaaaaagaaa aataggaaaa aagtttttgc cattctagga
     4861 agccttattc ttatagcaac tatctttacg tcttatttat ttaatccttc aaagactcaa
     4921 actattcata ttgctactaa acctatgact gaacaatata ttcttggtga gatgttggat
     4981 attttaattg aaaaaaacac aggtctaaac gtggaaatta cacaaggcat aggaggggga
     5041 acatctaata tccagccagc tatggagaat ggagaatttg acatttatcc agagtataca
     5101 ggtacaggct ggaatatggt acttaagaaa aatgaattat atactgaaag tatgtttagt
     5161 aaactacagt cggaatacaa aactaaactt aaaatgcaat ggactggtat gtatggtttt
     5221 tctaatagct atgcattagc agttagaaaa gaagttgcag ataaatataa tttaaaaaat
     5281 atatcagact taaaaaatgt atcttctaaa ttgacatttg gagctgaata tgacttttat
     5341 gagagagagg atggatatac tccattgtgt aatacatatg gactgaactt taaagataca
     5401 gttgatttag atattggatt aaaataccaa gctatcaatg aaggtaagat tgacgtaatg
     5461 cctatattta caactgatgg tcagtatagt gtttcagatg ttatagttct aaaagatgat
     5521 aaaaatttct acccatctta tcaatgtgga aatgttgttc gtttggaagt attagaggaa
     5581 aatccagaac tatcagaggt atttaagatg gttggagata ttttgactga taaagaaatg
     5641 gctaaaatga attatgacgt agaaaacaac aatttagaac ctagagaagt tgcttgtgag
     5701 tttctgaata agaaaggtct tttataaatt ggaggaaata taatgacacc tattatacaa
     5761 tttaaaaata ttaaaaaaca atataatgat aaaactataa tagataatct taatttagat
     5821 atagaaaaag gagagttttt gactgtaatt ggttcgtctg gttctggtaa gacaacactc
     5881 ttaaaaatga ttaatggtct gattttgcca gatggaggaa atattttgat aaaccaaaca
     5941 gatatcaaaa acgaggactt aattaaatta agaagaagaa taggatattg tgtacaagga
     6001 agtgttcttt ttcctcatat gactgttgaa gaaaatattt catatgtacc aaatctccta
     6061 agtaaaaaga ataaattaga agtaaaaagt gctgtaaaca agtggatgga aatagtggga
     6121 ttgccaaatg atatgaaagt gcgttaccca tcagaattgt caggtggtca gcaacaacgt
     6181 gtagggattg caagagcttt agcttcttct ccagaaattt tattaatgga tgaacctttt
     6241 ggtgcagttg atgagattac acgaaagcaa ttacaaaaag agataaaaga aattcataaa
     6301 aaaacaggag ttacgattat ctttattacg catgatattt atgaagcttt gattttggga
     6361 acaaagactt tggtattaaa tcatggagtt gttcaacagt atgatactcc tgagaatatt
     6421 ttaaatactc ctgctaacca atttgtagac cagttattga atataagaaa atctataata
     6481 gatgatgata atttaaaaga ataaatttaa atttaaaatc aaattgttgt gataaaaact
     6541 attgatttag agctaacttc aagttgtata cttgaagtgg aaaagttaga ttaaggtata
     6601 gaagagaatt tagcagttgg ctgtagacga ggtaactgct gaaggtgctt tcaataaatg
     6661 gctagaacct gtatctattg aagattataa taaattgaaa taagaagctc aatatacaga
     6721 gtagataggc acttcaaata gaagtgtctt ttgtgtgcca taaaataaat caaaatatta
     6781 gcaaagaatg tgaaaaacaa aataaaataa tatgctatct ttaaaatagg aggggtgcta
     6841 tatggataac aatttgatta ccattgaaaa aactttagat tatatagaaa aacatctttt
     6901 agatgagatg ttagatttaa atgtaatttc taaagagata ggctattcaa agtaccattt
     6961 acatagaatg tttacatcag ttgtcggatt tagtatacat aattatatac aaagacgtag
     7021 actaactgaa gcagcaagaa tgctcatttt tacagaaaaa ccaattattg aaattgcttt
     7081 aatctcgggg tatgacacac aacgttcttt ttcaacaggg tttaaaaaaa tgtttaagca
     7141 tagccctaag ttttttagaa aacatcaaga ttttttacca ctacaattaa aatttgatat
     7201 taataataga aaaaggttac atggaggtat ggttttagat gttaaaaaag ttaattgtac
     7261 agatataaat attgttggct attctggtaa agtaaaaaaa ggatttcatg ttattggaaa
     7321 atgttggagg gatttgcata aaaataaaga gttgatagaa aacaaggtag acttggattt
     7381 tttaattgga gttaatgatt atagtaattt taaagaaatt gaaaataatc ctacatttac
     7441 atatattgca ggtgttcaag tagctacttt tggtaaattc ctaatggaat ggaaaaattc
     7501 gtattaccga aaagtaatta tattgttttt acttttattg ggaaaaatga ggatagtatg
     7561 caaaatgttg tagaatatat ctatcaagaa tggttcctta attcaagttg ccagtttaat
     7621 gaaaataatt tatatgattt tgtaaaatac ggagaaaaaa ctgataagaa tggtttgagt
     7681 gaaattcagt tttgggttcc aatcatttaa aagaagggat tatgtataaa gtaggtggat
     7741 ttatataggc aaagttttat aaaagtatgt atttataaat aatttatagt ctgtaggaaa
     7801 gaaatagttt tactataaga ttatgataaa atacacaata ctatgataaa atagtactaa
     7861 ataatagatt taattttgca aaggataaag tgttttaata aaaatgaatg tagaaaacga
     7921 attacttaaa aacctagata gattgcatac aacagagtta ggcgttgtaa gaattaagaa
     7981 aaatctttct ttggagacaa atgatgtagt gaactggtgt aaaactaaaa tagaatctcc
     8041 aaatgccatt attaatagaa agggaaaaaa ctggtatata agtgtatatg attgtattat
     8101 aacagtaaac gctcacagtt atacaattat tacagctcat aaagaaaaga aatgaattga
     8161 aaaatacaaa ggtcaaaagc attttccact gtttgtagta cctatgaaga agtatataaa
     8221 ataatattac ttttgaataa taatttaaat tttggaggtt gaaaaaatga ataatactgt
     8281 aaaagaaaaa ttggcagagt cactaacttc tgatactact gatattttac cttatttgcc
     8341 ttatttatta caagattttt gggaattagg aagttcacca gaacaaatgt tacaattatt
     8401 tagaaagcat atacctttta atgaggatac aaaagttttg gatttagctt gtggaaaagg
     8461 tgctgtatct gtgaagcttg caaaagagtt gaatatacat atcaaaggta ttgatttgat
     8521 acctgaattt attgattatg cgagaagaaa ggctattgaa tataaggttg atgctttgtg
     8581 tgattttgaa atgaatgatg ctaatagttc tgtaaaaatt gagcacgatt atgattgtgt
     8641 gatttttgga gctgtagggg atattttagg taattataaa gaaactataa gtaagctatt
     8701 acctacaata aaaagtaagg ggttcatttt aattgatgat gcatatgtag tagataaatc
     8761 tacaaatgaa cagcttaatt ttgagagggt gtatcccact tacaatgagt ggtgtatatt
     8821 gttcaaagaa ttaggtctga aactaattga atgtatacca tgtttagaac ttgaaaatga
     8881 tgttgaagag gaaatgaact ggattataac tcgtgcaaat gagctaatta aaaaatatcc
     8941 ttctcaaaaa gatatgtttg aacaatatgt tattagccaa aaaaatgaat atactgattt
     9001 acagcaggac cttgttggag caacttggat tttacaaaaa gaataagata aatattagac
     9061 tagataatca atgttgtagt gaattatccc atgtctagtg aatataaata acataaaaaa
     9121 ccgtcaagat gaatagttta ttcgtgcttg acggttttta gtatttgttg aagtaaaatt
     9181 ttatttcttt agtatatcac ttttagtcaa tccatttgat ttagcttgag tattccattg
     9241 atgattcccc ataagtttat ataggaatct agggaagtta ggtgaaataa aaatagtact
     9301 tttgttggaa tcatctatat gtatattatc actaagtata ttttttgaaa gttcttttaa
     9361 tgcaattcct aaatcttttt taggtccttt acccaaaggt acggattcta gtgaaccaaa
     9421 catctcccca gctccaattc caataccttg aacccatgta aagttgattt ttttgcacca
     9481 atgatctatc atttgaattg ccaaatgatt atgttcacct tcattgaatc cacagttaac
     9541 tattgcatac actggaatag gttttttatt ttgtgttttt aaaaattttt cacatttctc
     9601 taaaaaatga agtaaatgag atggaatacc atctacataa agaggaaatg ctattattaa
     9661 tctatcacaa ttaagtatgg cattaagagc gtcaggatac ttagatgtgc tatctacttt
     9721 aaatgtaaaa actttattat cattttgaat aagttgtttt aattcagata aaaaatactg
     9781 tgaattattt ttatttactt ttggactacc atttatcatt actatattca tatggattca
     9841 cctcttatat cctgtatatt ttcatagaaa caaacagagt gttcttttat attgaaattt
     9901 atagcatttc catttactaa tttcgtagca gtttcttttt cagcttctgt aatacaatct
     9961 ccatagaata tagcttttaa atttatctgt ttatcatatc tgcttttatg acgcatcata
    10021 ttatctttta ttacaaagta tggaagaata taattaatgc ttttatcaag tacgtttttg
    10081 acaaaagggc taaaccctcc gtaacagcat ctgcttatta taataattcc atcggattca
    10141 tttataagtt tacccatgtt gctgtaataa tcttttacaa cacattcaga aggagtttta
    10201 atccagcaac caaaacgacc aatacatttg tggatttctt ttgtgtttgg aagtattttt
    10261 atattgtcat caagcttggg taataacttt tctaattctt tgtcagttaa atcatgtatt
    10321 aaaagcttca taaagtccat ctccttttat tatgtatgtt tatttctaaa aattatatct
    10381 tatagatttt tcagtaaatc tttacaccaa ttaataacaa aattataata gtcaactcca
    10441 taaatcattg tggcatacat atatgtatat tggtcaatac aatgattttc ttctacatca
    10501 attttttgtt tacttaattt atttagaagt aattctactt cagttattaa catttttaag
    10561 tttgtttcaa ttatttcttt gggtagatac ttgtaaaaga atatattaac aaggtggtct
    10621 tgctttgttt tagaaaaatt tataggtttc tttaaccatt cgagaaatac ttctttacca
    10681 gtattagtta tggaatatag tttttttaat ttgctaccat ctataacttc gtgatagtgt
    10741 atatatcctt ttgtttctaa tcttttaagt gcaggataga tacttccaaa gctggcgtca
    10801 aaaaagtacg aggtactttc tgacattatt tgttttaagt cgtatccact taattctttt
    10861 tccattaaaa atcctaaaat aatgtattct aacatattac ctcctggtat caatcagata
    10921 tatatcgatt cgatatattt attattacag ataataaata ttacgtcaag atgcttttta
    10981 aaataagcta aaaagaatta ggaattattg ataaatatgc aaaaaaatta atataaaagg
    11041 taattaaata tcaatgaaga tttaaaaata gttaaattaa agtaagagtc taatatataa
    11101 aatttatagt aactttatgg tttaaatata aacttgtgtg ctattgacaa agtataaaat
    11161 tagcagtaaa ataattgcat gagcaaatag attattgcga acgcaaaaaa atgaggacaa
    11221 tagatattaa aggagagaat atgagagagc tattaaaatg ggtttccatt ataaatagat
    11281 acaatacaaa ttatattgac aggaaattgg cggatattgg aatcaataat agccaatatt
    11341 tttatgtatt acacatatgt aataatccag gtattacaca ggacactata tttgagaaaa
    11401 ttcttgtgaa tcctagtaat ataacgagag ctttggcaaa tttagaaaaa gaaggcttta
    11461 taacaagaga accaggtgta aaggataaaa gaacatggca tttatatcct acagaaaaat
    11521 ctttagaatg ttatgatgaa atactaaaaa taacaaatgg gtatgttgag gagttgttga
    11581 gttcatttga gaaagaagag caagagttgt ttatgtcaat gttaaagaaa gttgcatttt
    11641 tagctattga tatgaatgaa gaagcaaaat tggaggcgaa ggaaaatgga agaaaaatat
    11701 aaggagccat tggaatatga aaaggtttca aagttattaa ctaagtacgc aattcctagt
    11761 attatagcaa tgttagtaag tgctttatat aacattgttg accaaatatt tattgggcaa
    11821 ggtgttggtg tattgggtaa tgctgctaca aatgtcactt ttccactaac aacaatatgt
    11881 actgcaattg cattattatt aggaattgga ggagcgtcta agtgttcatt ggaattaggt
    11941 gctaaaaatt cagaaaaagc tacaaaagca gcaggaaatt ctatatgctt gatggctgtt
    12001 tttggaataa gtttatttat agtagtatca atatttttaa caccactatt aaaattcttt
    12061 ggctctacat cagaaatact accttatgca cacacatata caaaaattac atcaattgga
    12121 ttaccatttc ttataatgtc cactggaatg agtaaattga tacttgcaga tgggagacca
    12181 aaagcttcta tgttttgtat gttaatagga gcagttatta atacaatttt gaatccatta
    12241 ttcatttttg tttttaatat gggaattgct gggtctgctt tagcaactgt tattgggcaa
    12301 cttatatctt ttggaataag tgttcattat ataaagaatt ttcaacatat taaattacat
    12361 aagaaaagtt ttaaattaga tagtataatt tataaacata tatttgcgct aggttcaagc
    12421 tcatgtttta atcaattagc aatgactgta acacagatag ttatgaataa tacattggca
    12481 tattatgggg ctaggtcagt ttatggaagt gagattccac ttgcttgtgt cggtatcatt
    12541 attaaagtaa atatgatttt tatatctata attattggta tatcacaagg tatgcaacca
    12601 attatagggt ataattatgg ggctgaaaag tataatcgtg taaaagaagc atataagttt
    12661 gcagtgggaa gtgcaactat tatatctgtt attgcattct tatgtttcca attcttccca
    12721 agacaaatta cgtctatatt tggtaacgga aatgagactt attttatatt tgcagaacat
    12781 tattttagaa ctttcttgtt cttaacattt ataaatggtg ttcaaatagt atcatcaact
    12841 ttctttacat caattgggaa atcagtgttg ggacttttta cttctttgac tcgtcaaatt
    12901 ttatttttaa ttccattgat agtaatttta ccaatgttta tgggaattga tggagtaatg
    12961 tttgcaggac caatagcaga tgcagcagcg gcaatggtat gtatatactt tgcagttcat
    13021 gaaatgaaat ccttgactat aaagcaaaat aatttgatga gtaaccataa taagattgca
    13081 gaggtataag acaatattta agaagtatag aaaattaatt taagaaatat aataaactaa
    13141 tttaggaagt gtagaaaatt aatttaaaga agtataataa actaatttag gaagtgtaga
    13201 aaattaattt aaagaagtat aataaactaa tttaggaagt atagaaaatt aatctaaaga
    13261 agtgtaataa actaatttag gaagtataga aaattaattt aaagaagtat aatgaactaa
    13321 tttaggaagt atagaaaatt aatttaaaga agtatagaaa attaatctaa agaagtgtaa
    13381 taaactaatt taggaagtat agaaagctaa tctaaagaag tataataaac taatttagga
    13441 agtatagaaa attaatctaa agaagtataa taaactaatt taagaaatat agaaaattaa
    13501 tttaaagaaa tacaatacat taatttttaa gtaacattaa attaatgtaa gtaaaataat
    13561 gttcatatga agatttaaag gtgcattttc tgaagctaag tatatgttat gtctctatat
    13621 ccggtgaagt ttaatagctc aagaaaatgc actatttatg atgattattt tgttttattt
    13681 ccaattttgg aaataactat tctaattaaa tctaaaatta taccataaat aagaaaagaa
    13741 agtatagatg ctatgtacca ataaatcgaa attgaccctg taattgttct acatgggatt
    13801 tgcatgcctg aatccataaa aacaaaaaaa ctatcaatgg tagaaataat agacaaaata
    13861 ggatttaatc ctattagaat aatacttttt gagtcttgac caatttggtg gatataaatc
    13921 tcaaataatg caattaaaaa aaacaaaatg taaaagattt aataattttt ttcatcaaaa
    13981 tacctcacct aatataaatt ctgtttgcac tatttgctta ctataactat ttatctccat
    14041 tcagtcacat cacttaattg taatctaggt cttcttgatg gttcttcgtc aggataacct
    14101 aaagatattg ctgccactaa ttgactatct gtatttaacc attcacataa ttcacgatat
    14161 gcaaagtata catcacaaat ccaaagacta cctagaccta attccacagc tgtaagcgac
    14221 atattctgaa ttgcagctcc aattgattgc atgtttgcca tttcatagaa tcgttcttca
    14281 ggggatagct tttctaaagg tgatttacct aatatattta aaataaaaat tgttactgga
    14341 gcttgtttca tgatttcaac tgtgtaattt gcaccagcga tatgttgacg acttcctgga
    14401 agaagcccat tatcattaat ttcattttga atacctttgc tcatggcttt aagcatagat
    14461 tctttttcct tttcagtgat gacaacaaaa cgccatggtt gcctgttttt agatgaaggt
    14521 gcatttattc cagcttcaat tattttctct atagtttcat gtgaaatgct ttgattttta
    14581 tattttctga tacttcgtct ttttgatata gaatcactta tcattttaag taacctccgt
    14641 ttattttttg gaaaaatcta tttattttct atttttaatt attttagcat taatcagaaa
    14701 aaaagtcatt gattttagta tacaattatt aacgattata ttaaggattg tatcaaaaag
    14761 tatgtgatta tactaaggga ttatattaga cattatattt aaaattgtat gaaaggttgt
    14821 atttaatatt atattaagag agatatatat taggatttat atatattaag atttattata
    14881 ttgagccctt atattaaatt aaggattata atttaaaaat aagctataaa aacccaatat
    14941 tgttttgttc agagataaaa aagaatactt taggtgattt ttattaaaaa ttaagagtga
    15001 taagattata tactttataa atgtaaatat gacgaataaa aatgataaat ataataatct
    15061 atatatttac attaggagtt ttctatacta taattattta tatttaaaac taaatataaa
    15121 tgttggaatt atttaggatt tgaaattgtc taaagataaa tataagtgat gaagaggtga
    15181 tgtgatgaga ttagatatgg atagtatgtt atatgtacta atttatatga cattattggt
    15241 atctatcatt ggaagtatat tttatttttg taaagacctc ttaaatcata aaattaagta
    15301 tgttttaata agagacatat ttgtaaatag aattacatat tctgaagaac ggttgcctaa
    15361 acagtatata gtttttcaga aatatgatat ttggaggtat tgtagtttgt tttaatttga
    15421 aatgaatcta ttataaattc taaagtttaa aaataaaaag gagagaataa aatggatttc
    15481 atttcaagta tattgcaagg attattaagt atattattta attttacagg tgattttgga
    15541 atttctattg tacttataac tttaatagtt aaattagtac ttatgccact atcattaaaa
    15601 caaagatttt ctgtaaaaaa acagcaagaa ttagctgaaa aaatggaaca tataaaagaa
    15661 aagtataaga atgatactaa agaattggaa aagcaaatac aagtaagttc tgtagaaagt
    15721 atgaagagta tgctgggatg tcttatagtt ttggttcaaa tgccagtagt atatgcttta
    15781 tatcatgtat gtttagatat gacaaaggaa tttacaacaa ttataattcc atggattgct
    15841 aatttaggta catttgataa tttgtttata ttaccttgta tatatacatt aattatgtta
    15901 gcttcaaatt tagtgaatta tataccatat ttaaaaataa attctcaagt aaagtttaac
    15961 aaacaaatgg ctgtggtgac taccataaca agtcttatac taactgttag aactccagta
    16021 gccataggtc tgtactttat aacaagtgca atttattcat taattgaaga tatttgtttt
    16081 agaatgtatt ttaatcgaaa aaatagaata gtgacaaaat aatataatta ttattagaaa
    16141 tattttagcc tgctaacata gaaaaagtta gtaggctatt tttatctatg atatcaattc
    16201 cattaagaat agtctaaaaa atactatcaa aatgctataa aaaatactat aaaaatacta
    16261 taaaaaaata gttgaaaaat aaataaatta atataaacta aaataaagcc tacaaaaaag
    16321 aggtgataaa tagaaatggc taaaatacta gttgtagaag atgaaaaaag gatgcaaagt
    16381 attattgtag aatatatgca aaagggagga tatacttgta taactgctaa tgatggagtc
    16441 gaggcactta caatattaaa gagcaacaat atagatttaa tgatattgga tattatgatg
    16501 ccgtatttag atggattttc agtgtgtaga gtttcacgtg aaatgactaa tatacctatt
    16561 atcatattga cagctaaagg tgaagaggag gacaaattaa agggttatga atatggcgca
    16621 gatgattata taacaaaacc atttagccca aaagtacttt tagcaaaagt taatgccttg
    16681 ttacgtagat acactactga tattcccaaa aattcattga gtttaggaaa gatttttatt
    16741 atggtagcat caaggcaagt gtatgtagaa gataaattga ttgatttaac atataaagaa
    16801 tttgaattat tgcgtttatt tatggaaaac cctaatcaag ttttttccag agagaagctt
    16861 ttaaattgta tatggggata tgattttgaa ggaaacacac gaactgtaga tacacatata
    16921 aaaaccttac gcaaaaagct tggtagtgaa ggacaccata tagttacatt aattcgttca
    16981 ggatataagt ttgaggttaa ggaatgaata gtttaagaaa gattatagaa aaactgaaaa
    17041 atgtagatat ttcaaattgg attaaacaaa attttggact atattcagtt agaaaaaaag
    17101 tgtttttact ttctaaatta tcaggagcgt taattatatt attttatctg gttgttgaag
    17161 aattttcgat aaactctaaa tttgaatttt ggatttggtt tataatactg gttatattta
    17221 ttatgagtat tgatttttta ttgggaaaat ttatttcaga accaatcaca tctataaata
    17281 aatctgcaaa aagtatgtcc caacttgatt tttctaatcc atgtactgta aatactaatg
    17341 atgaatttgg agaactttct aggagcctga atacaatgtc tacaaatcta caacaggcat
    17401 tatcagacct agaaagtgca aatattcaac ttgaaaagga tgtaaataag gaaagaatgc
    17461 tattggaaca aagaaaggag ttggtagatg caatttcaca tgaaatgaag actccacttg
    17521 gcattatacg tgcgtataca gaggggctta tggatgaagt tgatgaagaa aaaaagaaaa
    17581 attatatgaa tgttataata gaagaaactg atagaatgaa caatatgatt gtatcattat
    17641 tagacttatc tgcattggaa gcaggagttt ctaaacttaa cccagagcgt tttgatttta
    17701 tagagtttgt tgaaactgtg gcaggtcgtc ttttaattga tataccagat gccaattttt
    17761 attttacata tgatttgcca gagtatgaag tttttgtagt ggcagataaa atgagaatgg
    17821 aacaagtagt agagaatcta attattaatg caaagaagca tgttgtatat aatggtaatt
    17881 tagatttatc gattacatgt gaaaatggct tgcttttatt tgaaatttat aatgatggta
    17941 ggattataga gcaagatgag atttcaaaga tttggtctaa gttttatcgt agtgttcaat
    18001 ctcagcgtaa aggaggttca ggtctagggc ttgcaattgt ttcacaaata cttacaatgc
    18061 aagggttaga atatggagtg aataatcgtg atagaggagt agagttttat tttatgattc
    18121 caattgatga ataatttagt attaatttat aaattataag tatctgatgt aaaatatttt
    18181 tctttgtata cgtaagtatt taaagagaat aaaataaaaa acttaatgaa aaagtctaat
    18241 aaaaagttta atgaaaagtc ttgttttcct gatgaaaaca agatttttta ttaagctttt
    18301 tattatttca cttcgatttc acaagagaga cactctgatt tcaccaaata gaaataaaat
    18361 aggttaaata aattaaaaaa tagaagcgta attaaataag gttacaaggg ggagtactta
    18421 tgaaatggta tacatggttg actattatat tgataatatt gctttctatt ccgcttaaaa
    18481 ttaaatttat aaaatggtgg gatagacgac aaaaaaatga taaaggaggt tatgatagat
    18541 gataaaagta ggagagttaa aattttctta tggaaaagat aaacagatat tgcatggact
    18601 taattttgaa gtaaaagaag gagaaatctt tggattcctt ggacctaatg gttcaggaaa
    18661 atccacaaca cagaaaatct taaatggcgt tttaaaaggt tacaatgggc acgtatccct
    18721 ttttgggaag gaagttaaag catatacaga gtctctatat caaaagattg gtgttttatt
    18781 tgaatttcct tatttatata ccaatttaag tgcaattgat aatttagaat atttttcgtc
    18841 tttttatcct aaaaatcaaa ggagagacat atatgaactc ttagatttgt tagagtttaa
    18901 gaaagagttt ataaataagc ctgtatcatc ctactcaaaa ggtatgaagc aacgtattag
    18961 catggcaaga gcattggtaa gtaatccaaa gttgttattt ttagatgagc ctactagtgg
    19021 acttgaccca tctggagctg tactatttcg taagattatt gaagaagaac gaaaaaaagg
    19081 aacaactatc tttttgacta ctcataatat gctagatgca gatttaatgt gtaacaatgt
    19141 ggcttttatt gcagatggca agattatggt aatagataaa cccaaaaatt taaaaatgaa
    19201 aaatagcaat aataaagttg aagtggaatt tgtatataat ggaaatcgag atattgaaat
    19261 tgtagatata caagagttag aatctggaat aacttttaaa tatgatgaaa tcttaagtgt
    19321 acattcaaaa gaacctacac tagaagaagt ttttattaaa tacacaggaa ggatgttagt
    19381 gtaatgtatt ctaaattttt taccttagta aaaaaagatt tttggatgat gatttatggt
    19441 aaattttttg tgctgacact tggctctctc atactttatt cgtgttatat taattttggt
    19501 tatgtaaagt ttatggatga agatagttat cacaataatg tatatctata taatccagag
    19561 aatataagca tgagtgcatc tccattaatt aatcaagttt cttctatagg tgaattaaaa
    19621 gcaaaacttg atgaagatac aagtgcagtt tgtattgata tgagttccag taagccgaaa
    19681 gtaattttac atgaaagtat taaaggagtt gacaattata gagctgacta tgcattgtca
    19741 ttattatcat cagaaaaaga ccataatagc aaaatatcag acaacaatgc aataattcaa
    19801 attaatgata aggatacatc tgagataaaa caaagaaaag acatgacttg tgagatttta
    19861 ttctttgaaa ttgtagctat tggattttta gggattgcct ctttattatt taaagaaaag
    19921 cagatgggag taatgcgtat tactggtatt ttacctgtaa aaagaagtct ttttatatta
    19981 tcaaagatat ttgctttttt gatatgtgac ctatgtttta cagttctttt agtagtattt
    20041 aatattggat tttttgaagg agtaaaaata cttccacaag tattagttca agctagtatt
    20101 ttatcaatta ttatgtcttt gattggaata ggatgctcaa tgttattgag agattttaag
    20161 caattttctc ttatatatct tgtggttgct gtattcataa ctacacctat atttctatct
    20221 gcaaatacat cttttgaaat agattggatt aaatatcatc ccttctatca tgtttatagt
    20281 cagcttaaaa atgcgttttt catgaatttt tcaaaagaat tagtttattt tgtagtgtgt
    20341 tctttgacaa taatagttct atttttactt gtaaaatata tttttaataa agagttggtt
    20401 agggagggat aatcttgata ggattacgtt accagttaaa gagtataaaa aaagataaaa
    20461 tgtgtataat ttccttcttt ttaccagttg ttatggcgat tttaataaat tttatgggaa
    20521 caattgatat atcttcatta ggagagatac gttttggtat agtgaaggat acacttcctg
    20581 tacaaacaga gcagtggttg aagaagtatg gtatggtaag tgtatatgac acaaaaatgc
    20641 atttaatacg tgaaattaag gatattaaaa cggacacaat tggagttgtt agagatggta
    20701 aaggaataaa aactatgctt tatggaaatg agattaatgc tataaaaaat gttggaaaga
    20761 cattgccaga gatgtttaga ctaaagggtg atttaaaaga tgttgaggtt acaatattgc
    20821 ctaaagatag tattttaaaa gagtttcaga atgtttttat tgctatgatt ttaattactg
    20881 ctatgtttat gggatgtacc tttaatgcaa tgaatattat atctgaaaaa gaagatggaa
    20941 tatcatttat aaatgaagtt ttgccacaga gtagaagtca gtatattata caaaaagtat
    21001 ttttgggtct tttatgtggg tgtttatctt caatgataac tgtatttata tgtttggata
    21061 tttcaatatt agatatgata atgatgttgt tactcgttgt attttcagca tttatttcat
    21121 cacttatagg tttatttatt ggaaagttgt cagaaggtct tatggtagga attgtttata
    21181 ttaaaatagt aatgatttta tttataggtg ctcctttgtt aatatatctt ctaggggtta
    21241 ataccagcgg tatagggaat ttatgctata taatgccatc agttgctaca tttaatggta
    21301 ttatggagat tattaatggt aatgttatta ttataaaaga tattgtaatt ttggttgtac
    21361 attgtgtagt atggtttatg atatatatta ttttagataa aaaaagaaag ttatctttta
    21421 gtagataact cagtataatt ataattggga caatttaata ggtgatttaa tatactttgt
    21481 atagtgtaat ttatgtagtg caatttgtat actttaattt atgtagtgta atagttagtt
    21541 ttattttata a
//
LOCUS       DAGQWR010000071        21421 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.071, whole genome shotgun sequence.
ACCESSION   DAGQWR010000071 DAGQWR010000000
VERSION     DAGQWR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.071"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            62..658
                     /locus_tag="MIW95_002224"
     CDS             62..658
                     /locus_tag="MIW95_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBY3151548.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVNKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(756..1715)
                     /locus_tag="MIW95_002225"
     CDS             complement(756..1715)
                     /locus_tag="MIW95_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY3151549.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(1749..2480)
                     /locus_tag="MIW95_002226"
     CDS             complement(1749..2480)
                     /locus_tag="MIW95_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151550.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(2473..3243)
                     /locus_tag="MIW95_002227"
     CDS             complement(2473..3243)
                     /locus_tag="MIW95_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151551.1"
                     /translation="MFEKIHKRFKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(3328..4038)
                     /locus_tag="MIW95_002228"
     CDS             complement(3328..4038)
                     /locus_tag="MIW95_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBY3151552.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(4218..4370)
                     /locus_tag="MIW95_002229"
     CDS             complement(4218..4370)
                     /locus_tag="MIW95_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBY3151553.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(4396..6552)
                     /gene="feoB"
                     /locus_tag="MIW95_002230"
     CDS             complement(4396..6552)
                     /gene="feoB"
                     /locus_tag="MIW95_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBY3151554.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTIIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHVPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(6652..6873)
                     /locus_tag="MIW95_002231"
     CDS             complement(6652..6873)
                     /locus_tag="MIW95_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBY3151555.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(6886..7098)
                     /locus_tag="MIW95_002232"
     CDS             complement(6886..7098)
                     /locus_tag="MIW95_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBY3151556.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(7622..8230)
                     /locus_tag="MIW95_002233"
     CDS             complement(7622..8230)
                     /locus_tag="MIW95_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBY3151557.1"
                     /translation="MVDNLIKYIHKSNIAIVVLHEIYGVNNFIKDVCQKYYKGGYDVF
                     CPELLGEKRVFSYLKAQEAYDFFINNVKFDIYKKVEKLINGLKSEYKHVFVVGYSIGA
                     TIAWRCSENSLCDGIICCYGSRIRDYMDVIPTCPILLVFAKYDSFDVDYVSSQLSRRQ
                     NIQIEILDANHGFIDTYSKNYSNAQTEVFNIKEKNFLSKCLG"
     gene            complement(8350..8778)
                     /locus_tag="MIW95_002234"
     CDS             complement(8350..8778)
                     /locus_tag="MIW95_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY3151558.1"
                     /translation="MYGVIGATVLVIFVILTAGIMQGKKVDFQLPTVFMIVTFIVSAI
                     SEEIVFRGYIQTRLTGLIKNSVLSSCINAFLFLSTHYPVKWISSGDFSFAILSGFYVI
                     CLILLNFSCDLVYRKTNCLWGAWLLHILYNIGTGVLIFTT"
     gene            complement(8797..9057)
                     /locus_tag="MIW95_002235"
     CDS             complement(8797..9057)
                     /locus_tag="MIW95_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151559.1"
                     /translation="MFKTVFEDKEYVLLCKNYSKSDGVKAIFIYLISMLLLFFQGYLY
                     TTNLSSTILTNSQMILSILILLIGLGFVLLSKEKLNTIGITM"
     gene            complement(9331..9876)
                     /locus_tag="MIW95_002236"
     CDS             complement(9331..9876)
                     /locus_tag="MIW95_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBY3151560.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(9994..11817)
                     /locus_tag="MIW95_002237"
     CDS             complement(9994..11817)
                     /locus_tag="MIW95_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151561.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(11830..13605)
                     /locus_tag="MIW95_002238"
     CDS             complement(11830..13605)
                     /locus_tag="MIW95_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151562.1"
                     /translation="MKYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLREKIGVVFQNSSVFGKSILENIVMGNEGISREDVIKSC
                     EIANANQFITKLENSYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
     gene            13898..16126
                     /locus_tag="MIW95_002239"
     CDS             13898..16126
                     /locus_tag="MIW95_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBY3151563.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(16364..16663)
                     /locus_tag="MIW95_002240"
     CDS             complement(16364..16663)
                     /locus_tag="MIW95_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBY3151564.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QDLINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            16870..19911
                     /locus_tag="MIW95_002241"
     CDS             16870..19911
                     /locus_tag="MIW95_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBY3151565.1"
                     /translation="MFKQRISKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGNTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            complement(20072..21346)
                     /locus_tag="MIW95_002242"
     CDS             complement(20072..21346)
                     /locus_tag="MIW95_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY3151566.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
BASE COUNT         7460 a         3538 c         2465 g         7958 t
ORIGIN      
        1 atcattcata gtataatcta attacttgat attaattttc atttgaagga ggttatcttt
       61 tatgtcttgt tgtaattcac aattttgttg taaaaataaa attgaatcat catatataaa
      121 atggctttta gaggttttag gtccagtctt tttaggctct aaacctgctg aaataataaa
      181 tatttcgttt actgatgtaa atagagagga aaaaattaat gatatttata aatatctata
      241 taaatgtaag aaaatagatt ttatagttat tgataaagaa aaaaagggat taaaaatctt
      301 atttgtaaat aaaaaagcat tatcagaaaa attaaaatgt aaaaaaacag taaacttttt
      361 aaagttttta ggatacaaac aaaatacgaa tgttaatgct tatttagagc atttagtaaa
      421 taaacttaag agtgatgttt ttcctgatga aataggtatt tttttaggat atcctcttaa
      481 agatgtgatt ggatttatgg gatatagtaa ttatgaagtt tctatgatta aatattggaa
      541 agtttatggt gacacaaaac aatccgagga cacatattca aagtttttac ttcaccgtaa
      601 gaaaatgaga aaattactag actacataag tgtagataaa atagtaagtt gtttttaatt
      661 gtatttttgt ataataataa aaatacaccc tcttttacca tacagtttcc ataataaata
      721 agggtgtatt ttccacttta atattttaaa gtattctaat tttgattgta caacatatct
      781 ttaatattta ttttcttttg ttgcattcca ttagacatca aaaattcctg tgtatcttct
      841 aattctttta tatccttgtc cgttatttta gaactaaagt cataccaaga atacatttct
      901 tttgtttcat ctaaagataa accaacttct ttagatacaa catccatcac ttcatccttg
      961 ttttcattca tatattttaa agtttcttca tgaaccttca taaatctttt tactaattca
     1021 ggatattttt cagcaaagtc atcacttaca gcagtaacta ttattccact tactaatcct
     1081 tctccattta caatcatttt ggcacctgaa tttatcgttt tcaatgccac tggacctgca
     1141 agcattgcta catcagcact tttactttct aatgcagaag cagcactagg aatatccata
     1201 tttacatatt caacatcatc tatagtatag ccttctttag caagtgcagc aatcaacagt
     1261 tgatgaagta tagtcccttt tggacctaca atttttttac cttttaagtc ttttatagat
     1321 ttaatattat cattattagt tataagcata tatccttttg gtgaacggct ataaatattc
     1381 gtaattttaa gaccaacacc atttgatgca gctattattg cagacgttcc tcctaatgca
     1441 tgtaaaaagt ctagctcacc tgctgctaat gcttgagttt gctctggtcc tgttgtcaat
     1501 tcatggaagt tgacttttat attgtccttt ttaaattcct ttccaaataa atcatccttt
     1561 ttttctaata tactaggaac attaagtggt gattttacat aagttaaatt tatttcctta
     1621 ggtaaactag cctctttttt tgcaggttca tctttcttat catttgttgt tgaacatcct
     1681 acagtcccaa ccaacaccat tattaaagat gtcattactg ctaaaatctt tttgtttctt
     1741 ttaatcaatt aaaactcccc caattccctt aatatttctt ttttcaagtt tatataatag
     1801 tttttagtta aatctctatt atattcatct tcgacactaa attgtttaat ccttttattt
     1861 ttactaaaaa caattatttt ctttgctatt tccatagctt cttcaatatt gtgagttaca
     1921 aatactacac cttttttagt atttttatgg atatttacaa cttctttttg catttttctt
     1981 cttgtgaagt aatctagcgc tgcaaatggt tcgtccatca gcaaaatatc gggattgaag
     2041 gaaagtgctc ttgctatact tactctgtga gccattcctc ctgataattc atttggatat
     2101 gagtttttaa acttttcaag cttcatcatt tttaaatact tgtctaaatc aactttattt
     2161 ctattatctt tttcagtatg taaaagtata ttttcactta cattcagcca tggcataagt
     2221 ctactttctt gaaataccat tcctactttt ggagtacact ttttatcatt attaaaaaaa
     2281 tatatgtttc cactagtagc attttcaagc cctgctaaaa ttctaagtag cgttgttttc
     2341 ccacatccac tttcacctaa tataacagtt atctcttcac tagatatatt taaagatata
     2401 ttatcaagaa ctaaatgctc tttattatca actaaatatt tcttatatat attctctagc
     2461 aaaaatccac ttctaaccat ttaaatcaac ctgcttccct ttgctaaatt ttgttattat
     2521 ctttgaaaat agataatctg aaagtatacc taaaaatcca ataacaaaaa ttcctaccaa
     2581 tacaacatca gttctagaca tatcttttcc atcagaaatc aaatatccta gtcctgaaga
     2641 agctgcaata agctctgctc caattatagc cctaaaacta tacccaagcc ctaaactaag
     2701 tccaacaaca atatccagca tagaattagg gattattatt ttataaaaaa ttctcttttt
     2761 agataaatta aaagatttac ctacttctat taatttattg tcacaatttt ttataccttt
     2821 caatgtattt aaaaatattg gaaaaaagga tgccaaaata ataattataa ttttagattc
     2881 ttcacctata ccaaaccata aaataagcat tggcaccaaa gctagtggag gtgtacttcg
     2941 cataaattca aatagtggtt tgaaatattc atacattttt ttatttactc caaatacaac
     3001 tccaagtggt atagccatca atgaacttat taaaaaacct attaatatcc ttcttacact
     3061 ggcataaaca tttaagaaaa tagaaccgtc atatatcatt tttataaaag tatctaagac
     3121 tatttcagga ggaggcagaa tatagctact ccatataccc aaaacacata ctatcttcca
     3181 taaaattaaa attattaaaa aaatataata tcctttaaac cttttatgaa ttttctcaaa
     3241 catataaaac tccttttaac atatctatcc ctgctaccct ttttattttt taatatctta
     3301 aataataatt aagctatatc ataagattta atctatgcca agtcttaatc cacctgaact
     3361 cattctatca gaaggtgtag aatctgtttt taagccagac aatctttcag atggttgtat
     3421 aatgactatt tgtccaggtt caacaaattc catatgataa gattctcctg aagtctgacc
     3481 aataaaagtc ttccatgata gttttgatac tttaggatat ggttctcttc cagtccaagc
     3541 tactaatgca tctgggtcaa cacaacaagg tccttcaaga attagaggat taccatctgt
     3601 tattatagca acatcttgat tttgacctgt tttatttact aaatgtgtag taaaaagacc
     3661 tttttgagat attacccctg aaccaatcaa ttttacactg taatttaatt gttcagaaaa
     3721 tgccaataag ttttcactct caacataaat ggaatcacct gggtcaattg atattacatc
     3781 tacatggtaa gcattttgag ctaggtaaat ttcaccttca ccttcaacca ccattattgg
     3841 catttgttct cctgtcaatg acctctgaac atgacctaat aatgctcttc ccaatccacc
     3901 gccattactt ggacctaaca gcaatttttc aaaattaaaa tttcctttaa aggctaccat
     3961 agcacctttt tttgcaaaaa atttaccact tccccttgct atacacataa gctcgtttgt
     4021 aacatcaaat ttaaacataa tattcactta tattgcttaa tttagcaata atccctccta
     4081 atcatatatt tatatataat tatacagtaa aattacagca tagtctaacc tacttcaaat
     4141 atgaaatttg ttatttatat aaaaaataag aacctgataa taaagcaatt taacttttta
     4201 tcaggctctt atcctattta tctcttagcg ctatgacaac tcttactact tgagcatcca
     4261 gaacaaccac atccacaact gctaccattc ttttttgaat ttttaaccat atacattaca
     4321 gctagtgcca ttaatactac tacaatagct gctataataa atgtagccat attcatcact
     4381 ccttatttat atgaattaat ttttcatata acttaattta cccatttttt cttttttcat
     4441 atttttattt gaataagtta aaactacaaa gactactgct actgctataa atatagatat
     4501 tattgcccca gcaatacttc ctccagtacc taaaactaaa cttccaactt gatttataag
     4561 aagagcaatt atatacgctg ttaaagtttg gaatcctaat gtaatccatg tccatttcca
     4621 tgaacccatt tctcttttta tggctccaat agctgcaaaa caaggtgcac aaagcatatt
     4681 aaatgccata aatgcaaacg cactagctac tgtaaacata cttgcaactg aagcaagaag
     4741 tgttgggtct tgctctgttg catctgatat accaaataaa actccaaatg ttccaactac
     4801 attttcctta gctactagac ctgtaactgt tgctactgat gattgccaat ttccaaatcc
     4861 aagaggagca aatattggtg caactatatt tcctaagcta gctaatatac tatctccagc
     4921 atctaccatt tgtaatgacc aattaaatga ttgtaagaac cagattactc cacaagcaac
     4981 aaatataatt gttcctgctt tgattataaa tgcttttcct ctatcccaca tatgtattaa
     5041 aacacctttt gcacttggaa catgatattg aggtaattcc ataacaaatg gagatggttc
     5101 tcctgcaaat aaactagttt tctttaatat aattccacaa atgattatca ttgcaatacc
     5161 taagaaatac attgatggag ctacccatga agcacctcca aataatgccc ctgcaaataa
     5221 tgctattatt ggaatcttag ctccacatgg aatgaatgta gttaacataa tagtcatttt
     5281 tctatctcta tcattctcta tagtacgagt tgacataact cctggaactc cacaacctga
     5341 acttataagc attggtataa atgattttcc tgaaagacca aacttacgga aaattctatc
     5401 cattataaaa gctacacgtg acatataccc acaatcttct aatattgaca ataataagaa
     5461 taaaagcatg atttgtggta caaatcctag tactgctcca actccaccaa ttagaccatc
     5521 aactacaagt ccttgtaacc actctgctac atttaaagaa gctagaaaat tacttacatt
     5581 gccttgaata atttctccaa acagtacatc atttgtccaa tcagttgcta tagtacccaa
     5641 tgaacttact gctatatagt aaactcccca cattataagt gcaaaaatag gtaatgctag
     5701 tatacgattt gttacaatct tatctatctt atctgataca gtttccttac cttttcgatt
     5761 cttctttatt atacttgaaa ttattgaact aataaattca taacgattgg ctgttattat
     5821 actttcacta tcatcatcca attcatcttc acaatttcta gttatttctt caatactatt
     5881 caatattgtt ttagaaatat caagtttttg tataacattt tcatctctct caaatagctt
     5941 tatagctaac cagcgtgttt ctacatctat atatggagtc ttttcttcta ttattttttc
     6001 aattttttct attgcgtcct tactttcatc aacaaaaggt aatttgaaat ttaatttatt
     6061 gttactactt gcaagttcaa ctgccttttc agcagcctcc ataattcctt gacctttaac
     6121 tgctgttact tctaccactg ggcaacctat aacttcagat agttctttaa tatttatctt
     6181 atcaccattc ttatttacaa tatccatcat attaagagct ataatggtcg gtatacctag
     6241 ctctaaaact tgagttgtaa gatataaatt tctctctata tttgatgcgt ctacaatatt
     6301 tattactgca tccggcttat catctagcat aaaattacgt gtaactactt cttctaatgt
     6361 atatggagat aaagaataaa tacctggtaa atctactatt tccacatctt tatttccttt
     6421 taattttcca cctttttttt caactgttac tcctggccag ttaccaacat attgcgacga
     6481 gcctgtaagc ccattgaaca ttgttgtctt accacagtta ggatttccta tgagacctat
     6541 ttttattgac atttccattc cctccattat tatttaaata catcactgtt tgtgatacag
     6601 aaaatcattc tcattatata attacattaa ccctactcta attaatcttt attattccac
     6661 aataattttt tctgcatctg acttacgaat tgatagttca taatctctga ctgttacctc
     6721 aattggatca ccaagaggag ctacttttct tataaatata ttaacacctc ttgtaatccc
     6781 catatccatt atacgtcttc gtgtagctcc ttctccttct aactttttga cttttacagt
     6841 ctctccacac ttaatatcct tcagtctgtt catgtcatta cctccctata ttattattct
     6901 acttgccatt tttttatcta atgcaactct agtatctttt acgtctatta ttaagttacc
     6961 atctatctta gaaactattt taacatttgt acctactaca aaacctatac tatttaaaaa
     7021 agtcctcgtc ttatcacttc ccgaaatttt ctttataact acttcttcac ctgtatttga
     7081 taatatcaat ggcatcatat aatattcctc ctcttcaacc aatttaaatt tacaaacaac
     7141 ctctatctta tatataaatc acaatttctt aaattttgac tcgttaaaat aattacttat
     7201 ctttttcctt attcttacaa ttcctaattt tgatttaagt ctatcacaat agcatatttt
     7261 ttgcaagtga ttattgtatt cttttgattt tttttctcat ttgatttatt gtattaataa
     7321 aatctataaa aattcatact gctatcctat atattttgag atttctctta gatactcata
     7381 accatattct gcataagaat gttcttttga taaagatatt attctatagt aatgattttt
     7441 ttcttttcaa tgtaatttaa atattaatct aatacaatca taaaaacata attaattcta
     7501 tgattctact gactatctct atctcaaaaa atataatgac actactaata ttttttaaca
     7561 ttagtagtgt cattatgttt ttcaaattga ttaaattttc tatattttat aataaaacat
     7621 attagcctaa acatttagat agaaagtttt tttctttaat attaaaaact tctgtttgag
     7681 cattagaata gttttttgag tatgtatcaa taaaaccatg atttgcgtct aaaatttcaa
     7741 tttgaatatt ttgccttcta gaaagttgag aagaaacata atcaacatcg aatgaatcgt
     7801 attttgcaaa cactagcaaa ataggacatg ttggaattac atccatataa tctctaattc
     7861 gggagccata acaacaaatt ataccatcac acaaagagtt ttcactacat ctccatgcaa
     7921 tagttgcacc tatactatac ccaactacaa atacatgttt atactccgat tttaaaccat
     7981 ttataagttt ttctactttt ttataaatat caaacttaac attatttata aaaaaatcat
     8041 atgcttcttg agcttttaag tatgaaaaaa ctcttttttc tcctaagagt tctggacaaa
     8101 acacatcata cccaccttta taatactttt gacaaacatc ttttataaaa ttattaactc
     8161 cataaatttc atgcagtaca acaatagcta tattactttt atgtatatac ttaatcagat
     8221 tgtccaccac ctgtcgatat tttttacatc tagtttaaat tattttaagt atgtcaattt
     8281 actagctcta aaatttgact tataaaataa tccgtttctt acgataaact ttctgcaaag
     8341 taaaattgac taagtagtaa aaatcagcac tcctgtgcca atgttataca agatatgcag
     8401 tagccatgcc ccccacagac agttagtttt tcgatatact aaatcacatg aaaagttaag
     8461 taatataagg catatcacat aaaatccaga tagaattgca aatgaaaaat ctccacttga
     8521 aatccatttt acagggtaat gtgtagatag aaaaagaaaa gcattaatac aagatgaaag
     8581 tacactattt tttattaatc cagttaacct tgtttgaata taccctctaa aaacaatttc
     8641 ttctgaaata gcagaaacaa taaatgtaac aatcataaaa acagtaggta attgaaaatc
     8701 aacttttttt ccttgcataa ttccagctgt taaaataacg aaaattacga gtacagtagc
     8761 accaataact ccatacaaaa gacttttatt taaatttcac attgtaatcc caatcgtatt
     8821 tagtttttcc ttcgaaagta gtacaaatcc cagccctata agtaaaatca agattgacaa
     8881 tatcatttgt gagttagtta aaattgttga acttaaattt gttgtataca aataaccttg
     8941 gaaaaacaat agtaacatag atatcaaata aataaatatt gcttttactc catcactttt
     9001 agaataattc ttacataata aaacatattc tttatcttca aatacagttt taaacactat
     9061 aaatactcct ttctaattaa attcttattt atttctcata ttttatcata aatatatgtt
     9121 taaatcaaat taattctatt tattacataa atcagagata ataaaactaa aaaattaatt
     9181 tcattaaaac tatatttata atgatttaaa tatttacaag attatagcta taaaaaatat
     9241 aaaatacaac tattaaaaag gagcatatca ttataatttt aaaaatcata aatgatatac
     9301 tcctaaaaat ctacttaact tatattaaat ttattttcca aaatatctat ttaataatcc
     9361 taagaatgct ttaccatgtc tagcttcatc tttacacatt tcatgtactg tatcgtgtat
     9421 agcatctagt cctaattctt tagctttttt agctaatttt aattttccat cagttgcacc
     9481 atactcagca tcaactctaa ctcttaagtt ttcttttgta tctgcaacta caacttctcc
     9541 aagaagttct gcaaatttag cagcatgttc agcttcttca agagctattc tcttataagc
     9601 ttctcctact tctggataac cttctctatc agcttgtcta ctcattgcta aatacattcc
     9661 tacttctgta cactcaccaa caaagttagc tcttaatcct tcgattattt cttcatctac
     9721 accttgagct actcctattc tgtgctcatc agcccaattt ttttcgcctt tcatttcttc
     9781 aaatttatca gctccaactt tacatactgg acattgtgct ggtgcagcat ctccttcatg
     9841 tatatatcca catactgtac aaacaaattt cttcataatt actttcctcc taaattttat
     9901 atatcaaatt aaatttttta tttattaagt tagtctatat atacccgcac catatacctt
     9961 taaacaattt ttttaaaagt tttttctaaa tacttatttt ccctgtgtca ataatatgtt
    10021 tctataataa tgtcccttta attttatcaa ttctttatga tttcctgatt ccacaattct
    10081 accattttct aaaactatta tattatcaca atttgatatg gtacttaatc tatgagctat
    10141 aattatactt gtctttccat aagttaaatt atttattgct tcttgaattt tagcttctgt
    10201 tgctatatct atgttacttg tagcctcatc caaaataagt attggagcat tttttaatac
    10261 tgctctagca atacttatta attgtttttg accttcactt aaatcttcac ccctatccga
    10321 taatacagta ctatatcctt cacgcatttt aactataaat ttatgtgcat ttgctatttt
    10381 actagcatat ataacttctt catcagtagc ctccagattt ccatacctta tattttccat
    10441 aactgtatca ttgaatatat aaccttcttg tggaactact ccaaaacaag ttctcaaata
    10501 gtttctgtta atagagtata tatccttacc atctaaaaat atacttcctt catctatatg
    10561 ataaaaattt gtaattagat ttataatcgt tgtttttcca cttcctgtct ctcctatgat
    10621 ggcatttgat gtgccagctt tgatttttag gtttatatta tctaaaattt tcttttttga
    10681 atcataagaa aaacttacat ctttaagctc aatttctccc tttacaaaat gagagtttaa
    10741 attaccatct ttattatcgt taatctcact tttaaacatg ttatgcactt cttgctcttc
    10801 ttgttcatct ataatttcaa aaactctttc acatccagct atggctgaaa gtaatgtatt
    10861 aaatagattt gctaattcat ttaaaggtct tgtgaattgt cttacataac ttaaaaaact
    10921 agctataacc cctatggtta taatatcttt agatgcaaga acacctccta taatacatat
    10981 agttgcaaat cctatattac taatcacatt cataataggc attaaaagag aagtgtaaat
    11041 ttgcgattta agggaaatat ttagaagtct atcattgact tctttaaaat catttacaga
    11101 ttttctttca taattaaaag attttactac atttatgttt gatatcattt cttcaatatg
    11161 tgcatttagt tttcccaact caacttgttg ttgcttaaaa aaaactctag ttctctttgc
    11221 aataaaatta ctcacaaaca ttactatcgg tattaatatt acactgcaaa atgttaaaat
    11281 tgggcttaat acaaacatca taataaaagt catcaatatg tttaagattc ctgtaattaa
    11341 ctgtactaat gaatttgata ttcctgtact gatattatca acatcatttg tcactctact
    11401 cataatatct ccagtagaac ttttatcaaa aaaggttatt ggtagatttt taaattttaa
    11461 aaataaatta cttctcattt ctttaactat actttgagat aatctagctg tacatatccc
    11521 cttagacagt ttaactgaaa actcaattat ataaaatgaa agtaaaataa ttattccttt
    11581 taagaaaatg gattttgaat aagtatcgat atctattatg tcaactacct ttccaatagt
    11641 atatggtatt acagttgata taattgaatc aattattact agcaaaaaaa taaatctagt
    11701 aatcttctta tcttttttaa agtacataaa aattctttta aacgtttttt taaaatcttt
    11761 aggcttttct tttttcttta atctactgac cttttgattt ccagtactta agttcaatat
    11821 acttcctcct taatttacat cttcacttac taaattctgc aacgcaaaaa tttttttata
    11881 aattggagaa ctaaccagta aatcttcatg tgtagcaaat ccaaccacct caccctcatc
    11941 caagactaaa atcttatcac agtcaattat agaactaatc ttttgtgaaa tagttattac
    12001 agtacatttc ttactttcct tttctcttaa attacttata tactctttta tattctttct
    12061 tataattgtc tctgtattaa tatccattgc actaaaacaa tcatcaagaa ttaatatttg
    12121 tggttttttg gctatagctc tagctatagt aattctttgt ctctgtcctc ctgataaatt
    12181 tttacccctt tgaaataaaa cctcatcata agaattttcg agtttagtta taaattgatt
    12241 tgcatttgct atctcacatg attttataac atcttctcta cttataccct catttcccat
    12301 aactatattt tctagtatac tttttccaaa cacactgcta ttttgaaata ctacacctat
    12361 tttttccctt aaactttctt cagaaatact gtatatacta cgtcctccaa tcttaatatc
    12421 acctttatca attatatata atttttgaat taaatttact aaagaagtct ttcctgaacc
    12481 agtttgacca atcacaccaa tattttgacc ttcttctatt ttaaaggata cgttttttaa
    12541 tattttttca tctgtatttt tgttatacga aaaatctaca ttttcaaaaa ttatatcaca
    12601 atttacaaac tttaaatctt tttctccaac atctaaactt tcttctctct caattacttc
    12661 ttcattctca tcaaccaaaa cctcacttat tctctcataa gatgcttttg ccctaacaaa
    12721 aacattaaaa acatgtgaaa tcattataag agatgttaat attctagtca tatagtttac
    12781 ataagcaact ataatcccaa ttttaatttc tcctgaataa attaaatgcc ttccaatcag
    12841 taatattatt attatggaaa cattaatgat tattgtaatt gtcggtctaa aaaatgacat
    12901 catagttgta gcttttctac tatttttaaa aagttcatca tttaactctt caaagctttg
    12961 gttctcataa tcgtatctac aaaatagttt aactactttt attccactta aaaactgtct
    13021 taattttgaa tttactttat ctgtactttc ttgtacattt ttaaataatc tataccctat
    13081 cttcatatta agcaatatta taataaatat aattaaaact gcaattagaa taattattga
    13141 catctttaaa tttaatttta tagctataat taaacttcct atacacatta ttggtgactt
    13201 tataaaaact ctcatcattc cattaacgaa ttgttgcatt tgattgatat cattagtaat
    13261 tcttgttatt aacgaagctc taccaaattt atctatttgt tttaatgagt atgtatttat
    13321 cttttcaaat acatcctgtc ttaaatcata agaaaaatgc tgagaaactc ttgccgagat
    13381 tacacttctt attattgcaa atattgcacc taaaaaagtt atacctatca ttatagctcc
    13441 tgttgatagt atgtagtcta ggttcttatt tgaaacccct atatctatta tttttgccat
    13501 tatagttggt tgagctaaat cacaaaaagc ttccaatgtt aaaaaaaata ctgctgttag
    13561 aaataatact atatattttt ttatgtattt tttaaaatac ttcattcaat catcccttca
    13621 tagaattttc tcattaccaa aagtatatca taatggctaa tttaattgat tatatcaatt
    13681 ataaatattt gatttattat taatttttat accatatatt ttataaatta tttgttttta
    13741 tttcctatat ttatatatat ttatagcata ctattatata taatatatat gtattaaaat
    13801 taagacaaaa ttttaacttt actatttgtg taaaaagtaa tataatttaa aattgattat
    13861 aaatctaact tttttcttta tcaaaatatt ttaggaggtg agacatttga aaaaaactaa
    13921 tagtttaaaa gaaattttaa actggaataa tatcaagaat ttaagacctt caaagggttt
    13981 aaaaaatata ttagaaattt ttaaaagtga cctaaaagat atacgtggca attatgccgt
    14041 acttgtaata gttttaggtc ttgctttttt accatctctg tatgcttggt tcaatataaa
    14101 ggcatcttgg gacccttatg gaagcactgc taatatgctt gtagcagtaa caaatgaaga
    14161 taaaggaaat gaaatagcag gacaaaagat taatgtagga gaccaattaa ttgaacaact
    14221 caaagataac aagagtttag gttggcagtt tgtagatgaa aaaactgctc ttgaaggtgt
    14281 taaaaaaggt acttattatg catcacttaa gattccaaaa aattttacaa aagatttgac
    14341 atctttaatt acagatgatg ttaaaaaagg cgaaattatc tatactgtta atgaaaaaat
    14401 aaatgcagtt gcaccaaaga ttacagataa aggtgcttct acaatacaac tacaggttaa
    14461 tcagactata gtaaaaacag ttagtgaaat cgttcttggt atatcaaacg gagttggtgc
    14521 aggaattgaa cacagtcttc caaaattgac aaaagttgaa aattccctta ttgaccttca
    14581 aaagagattt gaccatataa ataagactgt ggatttagct tctgatgcta cttcaaaatt
    14641 atctgataca gttaaagata ttaaatctga tttacctaca attaaaaaga ctctgaatga
    14701 tacaaaacta ttatcaagtg acttaaaaaa attcttagag gatacaaatg ataatttaga
    14761 cgaactttct cctcttatta agtctgactt aaatttaatg gttgaccttt catcttctgc
    14821 ttcttctctt acattaaatt taattgatgc agtaaatagt ggcagtgaag atgtacctaa
    14881 gttgatagat aatttatcag aaaaactttc taatttgcaa tctttaaacg atactttggt
    14941 ggacttttta actaaattaa accaacttac atcaaataat agattggatg atgtaataga
    15001 taatcttgaa gactctagca ataaaataga ttcatctata tcaacattaa atgatattaa
    15061 aaacaaagtt atatctggac aacaaccatc tataagtgct ttgaataatg ttttaagtct
    15121 atcaaatggt attggaagaa ttaatttaaa tattttaaat aattttgact caaaaatatc
    15181 aaaacctata aatagtatat ttgcaaacag tataaaagta gcaaatgata taattactgt
    15241 tttagataag gcagaagcaa aacttccaaa ggttgaagaa atacttacta cttctctaaa
    15301 actttcagga aatgcacagg aaagtatatc attgataaga gaaagacttc ctttagcaaa
    15361 aggaatgttg gatgatttga ttgatacttt aagcaaaatt agtaatggtg aagatatgaa
    15421 aaaattagtc agcttgcttg aaagtgatat attgacaaag tctaatttct taaaagaacc
    15481 tgttgaggtg aaaacagaga aattatatcc aattgaaaat tatggttctg caatgacacc
    15541 attttatagt gttctttcta catgggttgg tatattactt atttcagcac tattgtctac
    15601 atctgtacat ggaaactata aaccttatga aatttacttt ggaagaggtc ttactttttt
    15661 aagtatagct cttgtccaag gttttataat aggaactgga gatatattat tgttaggagt
    15721 aacagcaaaa catccaattt tattgatagt tctgcttatg tttactagta tggtatttaa
    15781 ctttatagtg tattctttag tttctgtatt tggaaatgta ggaaaagcaa ttgcaatagt
    15841 actattagtc gttcagattg gttcttctgg tggtacattc cctatacaag ttacacctac
    15901 attcttccaa agaataaacc ctataatgcc atttacctat gcaataggtg ctacaaggga
    15961 agcagttggt ggtatatatg ctccaaatct aactaaagat atactagttc tattactatt
    16021 tatgattctg tctatttgtt taaacgtact tctaaaagga cctataaata gagttataaa
    16081 accatttaat gataaatttg gcgatagtaa tttaactggt cattaaaaaa ttaattctgt
    16141 ctttaaatat atttttttaa taatattcta agttcatatt aaaaggtagt agctaaaaag
    16201 ctactacctt tttccaactt aaaaactaat atttactaca aaaactgtca tagaaaaaag
    16261 cgaaaaaatt cgttctttcc taaatttatc ttataaatat ttagatatat tcctacttaa
    16321 taaacagagc tatacatagt atttaatgta taacttacct tatttaactc atataatcaa
    16381 ttattcctga atttaaatta tataaatgct caaaaccttc ttcagctaac aaattacatg
    16441 cagtaacact tctatgacca cttctgcaat aaactactac tttcttatct ttataatcgt
    16501 aaatttcatc tatattattg attaaatctt gtaaagatat atttatagca ttttctattt
    16561 tatattcctt aaattcatct ttagttctta catctaataa taaaacatcc ttttcatttt
    16621 tcatcatttc tttaagttgc ttcttattta tattcttata catatgtcta aaattcctcc
    16681 attttattta tatattacct atacccttta ttctttatac aacaacaaat attattttta
    16741 tatttttctc tgttatataa aaagctatac ttataaaagc tttttttatt gataataatc
    16801 tgttgattaa aataattaag aatgttatgc taattataaa ttattaaaaa gggaaagagg
    16861 taatgtaata tgtttaagca aaggatctct aaattattat ctagtacatt agtattgagt
    16921 atgttattta ctgctgctcc aaatattact tttgctgata atacaaaaga taattccgaa
    16981 aaatatcaaa gttctgatat agaattacat gattatagta aaaacgcgga atcttacaca
    17041 aaaactaaag ctcttgctaa agagaaaatc caaactttgc tatcaaagta tggtgctgta
    17101 agtgctcaat atgctttgat tgataatggc aaaattgaga tttctggtaa tggtggagtt
    17161 tacagcaaac aggataacaa aaacttaaat aaggataata tgtatagtat cgcatctatc
    17221 agtaaaatgt ttactacaac agctgttatg aagttggttg atgatggaaa attaaattta
    17281 gatactcctg ttgtaaagta tatacctgaa tttaaaatgg ctgatgatag atacaaggaa
    17341 atcactccaa gaatgttatt aaatcattca tcagggttga tgggaagtag ttttaaaaac
    17401 actattttat tagcagacaa tgattcttat ggtcatgata atttcttaaa agaattacaa
    17461 aaacaaagat taaaagctaa accaggagct ttttccgttt attgtaatga tggatttact
    17521 ttagctgaaa ttttagtaga aagagtatct ggtatgtcat ttactaattt tcttgataaa
    17581 tacataaata atccacttaa cttacaaaat acaaaaacta cagaaaatag ttttgatagt
    17641 tcaaaactag caaaagcata tgtaccttat tgggaagatg cagttccaca agataacctt
    17701 aatgctatag gtgcaggtgg tttatattca agtgcagaaa atctatgtac atttgcacaa
    17761 acatttatga aaaattcaaa tggtattctt tctcctgctt cagtaaaagc tatggaaaac
    17821 aaagaatatt taaatggtct atggcctgaa ggagaagatt ctatacttgg atatggttta
    17881 gggtgggatt gtgtcaacac ttatccattt aatcagtata atttaaaagc tcttacaaaa
    17941 ggtggagact ctttattatt ccacagtaat cttatagttc ttccagatga aaatatggca
    18001 gttgcagttc tttcttctgg tggaagtagt caacttaacg aaataattgg acaagaaatc
    18061 ttactatctg cattaaaaga aaaaggtaaa attaaagaaa taaaacctga taaaacattt
    18121 agtaagccac aacaagttaa aatgcctagc tctctaaaag aaaatagtgg tctttatgct
    18181 tcttcaaata tgataaaagt agatgttaat gataatggta ctttaacagt atcttctcct
    18241 tatatagaaa atggacctga agataaatac gtatacatag gtcaagatag atttgtatct
    18301 gaaaagggta acagttgttt aaaatttgta aaagaaaaaa ataatatcac atatttaaat
    18361 atgagctctt atgatgatgt ccctggatta ggtcagacag caagtttata ctatgtagct
    18421 caaaaagtag atgacaacaa tatatcaaat agtgtcaaag aagtgtggaa aaaaagaagt
    18481 ggcaaaggtt actatctagt ggatgaaaaa tatacttctc aaagctatat gtttggctct
    18541 gtaaaagcaa gttttagtct ttctgatgaa actccaggtt atatagttaa cactaaaata
    18601 atggatgaga ataactcaaa tgcttttata gaaatacctg gtgtaatagg tcgtgattta
    18661 tccgacatta aacttcataa agaaaatggt acagagtatt tatcttttgg aacactaacg
    18721 tatgttagtg aagattctat aactaatttg ccagcagaaa aatcttttac ttgtgaacta
    18781 gaatccaatg gttatgcaaa atggtataag attggtgacg atatcgcaaa taaaaaaata
    18841 gaagttaatc ttcctcaaaa ttcagctttt gctgtttatg atgataaagg tgttcctgta
    18901 aactattctt tagttacaaa gaacaataga gttagattgc caaaaggagg cgtaattgtt
    18961 ttcttaggaa gtccaaatgc tagatttgag gttacatatc aagatgaagt aaatgcaagt
    19021 gcacttacag gaactgatag atatgaaact tctattaaga taagtcaagc aggttgggaa
    19081 aatgctgaaa atgcagtttt aataaacgat tcagcaattg cagatgcttt ggccgcaaca
    19141 ccatttgcat acaagaaaaa cgctcctata ttattgactg gaagctctca aataaatgaa
    19201 aaaactttgg ctgaattaaa gagattaaaa gttaaaaatg tatatgtagt tggtggagag
    19261 gcttctataa atgaaaaatc tttagatact ataaaatcaa acaatatctc tgtatctaga
    19321 atttcaggaa gtgatagata tcaaacttct atgaacattg ctaaagaatt gaacaatatt
    19381 agcaatatat caaagatttc agtagttaat ggcgaaaaag gattagccga tgcagtaagc
    19441 attggagctg tatcagctca aaatgatatg cctataatac ttactaatga aaatagtaat
    19501 ataactgaaa taaataactt atttaaaaac aaaaagattg ataaatctta tgtaatcggt
    19561 ggagaatata cagtttctaa aaatatagaa agtaaacttc aaaatccaca gagaatatca
    19621 ggtaacacta gaaatgaaac taatgcaaaa gtaataaaag aattttacaa ggattctaag
    19681 atagacaatt tatatgttgc taaaaatgga atgaataaac aagatgattt aatagacgga
    19741 ttatctgttg gagttttggc tggaaaaact aaatctcctg taatgctagt aggaaactca
    19801 ctagattata atcaaaaaga actatttaaa actatgagat ttaaatctgt aacacaaatt
    19861 ggtggaaatg gaaatgaaaa ctcatttaag caaataaaag aaatagctta acataactaa
    19921 aaaaggctgt attcaagtaa tattaattga atatagcctt tttttattta agttataagt
    19981 tttatatgtg taaaaattaa atttaaatat taaatgataa gtttattaag atatagataa
    20041 gtctaaaact ttgccttttt taagttagtt tctatttatc attcatactt aacaatttat
    20101 ctaaatatac tgactttaat tgtttactgg ctaaaatctt tttagctggt tcaagcttta
    20161 atatagctga cagtatagct cccatagctc tatgactagc cagagcatta ttatcaaaaa
    20221 ttaaattttg taactgccct ttttcaattg ccatgagaac tgctctatgt actgaatttg
    20281 ctggagttat tattttttca tctctcttta ataacataat gctattttta tggcaatttc
    20341 taacacaaac tccacaacct aaacatctat cctcatcaat ttttatatat tctttaccat
    20401 cttccttaac cttgcttatc gcatctattg gacatgcagt tatacattta ccacacttta
    20461 cacaattttc atggtttata tttggtataa aacttgttgt ctgtactgga tgaagatttc
    20521 caaatctttt agctgctacc attgcttcac agcaacatcc acagcaatta catataaacg
    20581 taaccccttt cctaacattt tctccacatt gaactaagtt atgttcatat gcttgatgaa
    20641 gtagttcttt acattctatt ttatccacac gtcttgcaaa tttattattt attaaagaat
    20701 ttgctacatt gtcaaacgtc atacatatat ccatgggagc atcacaagct tttccaacat
    20761 gttgcattct atgtctacag taacacatac ttatacctat atgagttgat tcatcaatta
    20821 tatgagtagc cctttcataa tctaaaatac taacctcatt atcattagtt aaaacttcct
    20881 cttgaacgta tactcttcca agcttcgttt ctgttgaata aaataaatct ttgataaaat
    20941 cttcttctac attcatatat tgatagtaaa gttctgccaa aagtttttgg tctatatcgt
    21001 gtctcgttct catcatggca aactcaaaaa aaccagccat tggaggaggc attatatatt
    21061 tcttcccttt attatcttct atatctagta tcagagcctt actagcaagt ttatccaata
    21121 ctctatatgc ttcactttca cttacactcc aaattttagc tgctgttttt actctgaaag
    21181 gctttattgg aagctgagca actaacttcg cctcttgttc tgtatatagt acatttagta
    21241 ttttgtataa tgtatcagat ggtggagcac cttgtggaaa cttgtttatt ctttcctcta
    21301 aacttttata catattcttc gcagttatat gagaaatagt aatcaccttc tttttctttt
    21361 gttttcaaat aaaatcaaat attctttctt attatatatt ctcttacttt atataatacc
    21421 a
//
LOCUS       DAGQWR010000072        21397 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.072, whole genome shotgun sequence.
ACCESSION   DAGQWR010000072 DAGQWR010000000
VERSION     DAGQWR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21397
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.072"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            163..1485
                     /locus_tag="MIW95_002243"
     CDS             163..1485
                     /locus_tag="MIW95_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBY3151567.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            1783..3144
                     /locus_tag="MIW95_002244"
     CDS             1783..3144
                     /locus_tag="MIW95_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBY3151568.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            3217..4611
                     /locus_tag="MIW95_002245"
     CDS             3217..4611
                     /locus_tag="MIW95_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY3151569.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQLAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            4673..5143
                     /locus_tag="MIW95_002246"
     CDS             4673..5143
                     /locus_tag="MIW95_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBY3151570.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIKEAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            5146..7362
                     /locus_tag="MIW95_002247"
     CDS             5146..7362
                     /locus_tag="MIW95_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY3151571.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFRGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLIVEEAEKLLETSKEDLYLDNGYVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGLTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVNSYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            7445..8116
                     /locus_tag="MIW95_002248"
     CDS             7445..8116
                     /locus_tag="MIW95_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY3151572.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            8479..9693
                     /gene="dpaL"
                     /locus_tag="MIW95_002249"
     CDS             8479..9693
                     /gene="dpaL"
                     /locus_tag="MIW95_002249"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBY3151573.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            9820..11007
                     /locus_tag="MIW95_002250"
     CDS             9820..11007
                     /locus_tag="MIW95_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBY3151574.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            11140..12510
                     /gene="hydA"
                     /locus_tag="MIW95_002251"
     CDS             11140..12510
                     /gene="hydA"
                     /locus_tag="MIW95_002251"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBY3151575.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKN
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            12528..13934
                     /locus_tag="MIW95_002252"
     CDS             12528..13934
                     /locus_tag="MIW95_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY3151576.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            14006..14452
                     /locus_tag="MIW95_002253"
     CDS             14006..14452
                     /locus_tag="MIW95_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBY3151577.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            14491..15285
                     /locus_tag="MIW95_002254"
     CDS             14491..15285
                     /locus_tag="MIW95_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBY3151578.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            15275..17566
                     /locus_tag="MIW95_002255"
     CDS             15275..17566
                     /locus_tag="MIW95_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY3151579.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDTKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            17567..18880
                     /locus_tag="MIW95_002256"
     CDS             17567..18880
                     /locus_tag="MIW95_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBY3151580.1"
                     /translation="MIAIKSKYLISSADEIYTNSAAVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            18916..20001
                     /locus_tag="MIW95_002257"
     CDS             18916..20001
                     /locus_tag="MIW95_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBY3151581.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            20231..21322
                     /locus_tag="MIW95_002258"
     CDS             20231..21322
                     /locus_tag="MIW95_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151582.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEMTVMKEDISEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEINVLKEDMSEVKQ
                     EMIVMKEDMSEVKQEMTVMKEDMSEVKQEMTVMKEDMSEVRQEINIMKEDISEVRQEM
                     TVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINV
                     MKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIE
                     IKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKED
                     IAEIRRDLNLVELATSKNWSDIVKLKSVK"
BASE COUNT         8295 a         2377 c         4385 g         6340 t
ORIGIN      
        1 atatgtaaaa caattatgtt aaaaaattgg cattataatt gcttcatatt aaattgagat
       61 gacaaaataa aaaaagaaag gatgttgtgt gagataataa taactgtcat taaaaaatag
      121 ttgggagtga taaccttgag taaatatcaa aagaatgttt ctatgtttga tattgatgga
      181 aggccaccaa ttttaaagtg tttgccacta tcaatgcaac atatactagc aatgatagtt
      241 ggaactgtta cagtacctat agtcgtagga ggagctgtgg gtgttagccc tacacaaata
      301 acacttttag tgcaatatgc attaataata gctggtatat caacacttat acaactatat
      361 ccaataggaa atattggttc aagattgcct ataatttttg gagttggttt tacttatgta
      421 ccaacacttg tagcagtcgg aggaaattat ggattagcaa gcattttggg tgcacaatta
      481 atagggggag tggctacagt attaataggg atattcataa aaaagataag aaaatttttt
      541 ccgaatatag ttgcaggaac agtggttttt acaattggat tatctttgta tcctatagca
      601 ataaattata tggcaggtgg tgtaaatagt cctacttatg cctctatgca aaactgggca
      661 gtagcggcta taacattagt ggtagttata ggtcttagtc aatttgcaaa aggatatgca
      721 aaattggcag caatattttt gggaattata gttggatatt gtgtatcttt agttctgggt
      781 ataataaatt ttgaccctat tagagaggca acttggtttg cgatacctaa accgcttgaa
      841 tttgggatgg aatttgatat gcctgtaata atctcagtag taattgtaag tataatcaat
      901 gctgttcaag caattggaga tttatcagct actacaatgg gaggcatgga taggaatatc
      961 acagataagg aattatcaag tggagtaata ggaagtggaa tttgtactat gataggagct
     1021 ttgtttggag gtttaccacc atcatcatat agccaaaacg tggggatggt agctatgaac
     1081 aaagtaataa gtagatttgt gcttggaata gctggtatat ttatgttatt gtcggggttt
     1141 gtgcctaaat ttggtgcatt aatgactact ataccatatt ctgttttagg aggagctaca
     1201 atatctatat ttagtatgat aacaatgaca ggtataaagt taattattac agaagagttg
     1261 tcatcaagaa atattaccat tgttggtttg gcaattgcct taggcatggg aataacatca
     1321 gtaccagcag cccaagaggc atttccttca tttgtaaagt tagtatttgg agaatctcca
     1381 atagtaattg caacattggt tgcattcgtt ttaaatctaa taataccaaa taaaagtcta
     1441 gaggatgagg ctaaagaaag agctgttatg gaagagacta attaagatat aataatttaa
     1501 aagaattgtt taaattattt agtatgagat tgttagatta tttttgatag ctattcattt
     1561 taataaaaaa tttaacaaat aaatatatac caggcaatta atgtttcatt taagttgaaa
     1621 atcggggagg gaatatttag aaatttgtta attttttata aaaatagctc agtttagctg
     1681 tgtttatcgg ttgagcaata aattcatggt cataaaaata tttatagaaa ataattataa
     1741 aaatatttag tcaagatatt tatgtatggg aggatttatt ttatgaaaac acgtaatact
     1801 tcaaaatatg atgtcgatgg gataccacca ctaaaggaat caataccttt ggccttacaa
     1861 catctgttag caatgatagt aggaaatatg gtacctgcga tattaatagc aaacgtggta
     1921 ggacttaacc aggggcaagc aacaatgttg attcagggtt ctatgttagc agcaggttta
     1981 gcaacgtttt tacagttata tccaatacct ttatttaaag gatttaagtt aggttcaaga
     2041 ttacctgtaa tgatgggaat gagttatgta tttttaggag cttgtctttc agtagcagct
     2101 aaagatggtt tagcagctct atttggagca cagattgctg ctggggtaat cgtattcttt
     2161 gtaggatttg gagttaagaa aattagacat atattcactc caatagtttc tggaactata
     2221 atagcatgta tgggacttgg actattcgca acagctataa agaatttagc tggaggagaa
     2281 ggcacacaaa catttggaag cccaataaac ttcacagtag gagttatagt tgcttttgta
     2341 ataataatga taaataaata cggtaaaggt ttagtaaaaa attcttcaat attaatagga
     2401 atattagtag gttatgctat atctttagtt ttaggattgg ttgatttctc agccgttcaa
     2461 ggagcagcta tagtatcttt accaacacca gcagcttttg gattagaatt tagaccagag
     2521 ttaatagtaa tgtttactat tatttacata ataggtatag ctgatatgat gggtgcctgt
     2581 acaatagcta cagtaggagc aatggataga gaggtaactg acgaagaatt agcatcagtt
     2641 gttttaggaa attctataac ttctataatt tcatcactat ttgcagctct tccaactgga
     2701 gtatttagcc aaaatacagt aattgtatct atgaacaaag ttacaagtag atttgtaata
     2761 gcactagggg cacttgtatt attactagca gggatatctc cagcattggg tgcaataatg
     2821 acaactatac catcttgtgt tgttggtgga gcaactttag tagttttctc atcaatagca
     2881 atgtctggtt tctctattat gagtatggat ggattcactg aagaaaacaa tcttatagca
     2941 ggtgtatcta tagcaacaag tatgggtctt acaactgctc cacaagtttt agaccaattc
     3001 ccagaaacaa ttggaactgt ccttggaggg tcaagtatag tttctggagc aataatagct
     3061 atattacttc aaacattatt taaattaaag tcacgtaaaa gtgctgaaaa cgttacttca
     3121 aatcttgagg aaaatattgg ataaatgtat tagatataat atgagaaaac catatggtaa
     3181 aatgttgtaa tgaaagtatt caaaggggga tttaagatga ttgatatact aataaaaaat
     3241 gcaataattg ttacagttaa taaagacaga gatgttatat ttgatggagc aatagctatt
     3301 caaggtgaca gaatcttgga tataggaaat acagttgata ttgagcctaa gtatcttgat
     3361 gctaaaaaag ttatagatgc taatggaaaa gtaatattcc ctggctttat aaatactcat
     3421 aatcacctgt tccaagtgtt attaaaaggt ttaggtgatg atatggcact tcatgagtgg
     3481 ttaaatacaa tgatgtttcc atcagcaaaa ttcttaacag aacaagatac ttatgatgct
     3541 gcaatgcttg gatgtatgga aggtttaaaa agtggtataa ctacaatggt tgattatatg
     3601 catactcaca atagaccagg acttacagat ggaatagtaa aggcatataa agatttaggt
     3661 ataagagggg ttgtaggtag aggttgtata gatttaggta ttcacaaaga gcttatagaa
     3721 gatgtggaaa cggttgaaaa agatttgaga agggtattcg aaaaatatca taattcagaa
     3781 aatggaagaa taaaaatctg tgtagctcct tcttcaatgt gggctatatc tgaagaaatg
     3841 ggtaagatgc tttggaatat agttaaagaa tatgattcat attttacagt tcatatatct
     3901 gaaacagaat ttgcaagaac agcaacaaag gatatacatg gagaaataga tataaaatta
     3961 cttgaaaaat ggggtatagt aggtccagaa gttgtagcag tacattgtgt atgtataact
     4021 gatgaagaca tggaaatgct taaaaagtat gacataaaag tttctcataa tgtagcaagt
     4081 aatatgtatc ttgcttctgg tgttgcacct gtgccagaaa tgttaaaaaa aggtataaat
     4141 gtgagtttag gtcttgacgg tgcagcaagt aataacgcac aagatatggt agagcttatg
     4201 aaattaacag ctttacaaca caaagtaaat aattgtgacc ctctggcaat ttctgcagag
     4261 aaagttcttg aaatggctac aattgaggga gcaagaactc ttagaatgga agatgaaata
     4321 ggttctttgg agattgggaa aaaagctgac ctagtaatat ttaactcaat gctttctcca
     4381 aaatcaatac ctcttcacaa tcctgtatca actttggtat actcagctag tatgcacaat
     4441 gtagagagtg ttttagttga tggaaatgta atacttgaaa atggaaaagt aactacaatt
     4501 gaaagtgaag aacaagtgtt gcttaatgca caacttgcag cagaagaatt atgtaagaga
     4561 gctaatgtaa ctaatagaat ggaaggacat aagtggaata gcttatacta atattaaaat
     4621 aagattgtaa taatattcta ttacaacatt agtttatctg taggaggaga aaatggaaaa
     4681 aataaaatta aatattaatg attacatgta tgaaatagaa gttgaaaaga atgaaactct
     4741 tttgcatgta cttcgtgaaa aattaaatct aactggagcg aaacaaggtt gtaatagtgg
     4801 cagttgtgga gcatgtaaag ttttgataga tggagttgac atgaaatctt gtaaacttct
     4861 tgcaaaaaac tgcgaaggta aagttatata cactatagaa agttttgcta atggagaaaa
     4921 tcttcatcca atacaagaag cttttataga agttggagct gtacaatgtg gatattgtac
     4981 tcctggtatg attataacaa cacaagctct tttaaataga aatgcatctc ctaatgaaga
     5041 tgaaattaaa gaagctttta aagaaaatct ctgtagatgc acaggatatg taaagattat
     5101 agaagctgta aaattatctg cagctagatt agggggtaga taattatgga aaaggtaata
     5161 ggaaaatcat accctctaaa agatgcagct ttaaaagtta ctggtcaaat gaaatacgtg
     5221 gctgatatga aaccacaaaa tgtactttac gctaagatgc ttttaagtcc tgtagcacat
     5281 gcaaaaataa aaagtataga tacaagtgaa gctgagaaag tagtaggtgt tagagctata
     5341 gctacttatc tcaatacatc acaaagaaaa tacaatggtt cattaagatt ttatgagcac
     5401 aatatacctg aaaatgaagt tatattttca gatacagtta gatttgttgg tgaccgtgtt
     5461 gcagcagtag ctgcagagac tccagaaata gcagagaaag ctgtaaaact tataaaagtt
     5521 gaatatgaag aattgccagc tgtatttact atacaagatg caataaaaga agatgctatc
     5581 tgcatacatg gagattcaaa tatagtttct caaaacaaca taaatggggg agatgtagaa
     5641 aaaggcttca aggaatgtga ctatatattt gaagatgaat actctactcc agcaatacat
     5701 catagtgcaa tagagactca tagtgtaatt gcacactatg attataatga taaattaact
     5761 gtacatacac cttgccaaaa tacatttgga tttagaataa tactatctca aatttttggg
     5821 atgtcattaa ataaagtaag agttataaga cctgctattg gaggctcatt tggtggaaaa
     5881 ttagaactta caattgaacc agtagcagcc gcattatcaa aaatgacaag aagaccagtt
     5941 aaaatggttc ttacaagaaa ggaatctatg atatcaacaa gaactcgtca tgggtctttt
     6001 gtaaaattaa aaactggtgt aaataaagat ggtgaagtaa tagcacaaga tataaaaata
     6061 tatacaaata caggagcata tgcatcaagt gctttaaatg ttataggagc acttagtcat
     6121 aaagtattta aagtttataa aataccaaat ataaagttta ctgggatgcc tgtatataca
     6181 aatacaccaa tagcaggagc tatgagaggc tatgggtcac ctcaaatatt tatggctcaa
     6241 caggctcaat ttgcgaaaat agccaaggaa ataggtatgg atttagtcga ttttcaaaat
     6301 aaaaatgcag tagagccaga tgatgtcgat ataatcttcc gcggttctct tggaaatcct
     6361 agagtattgg attgtataga gcaaggtaag aagatgttta agtgggatga aaagaaaaaa
     6421 cttccaaaag aagaaggaaa atatttaaga ggtataggta tggcaatagg tgcacatggt
     6481 aatggagttt ttggtgcaca tagagatgtt gttacattga ctcttaaatt aaatgaagat
     6541 ggtacattga cactactaac aggagcacat gatatgggta atggtgtcgt aactatgcaa
     6601 acaatgatgg tagctgaagt tttaggaata acacctgata aagttgatac atttgaaact
     6661 gatacggatg catgtacatt caaccttggt gactatgcaa gtagaggggt atttgtagaa
     6721 ggtggaggag ctaaaaagac agcagagaaa ttgaaaaacc ttatagtaga agaagcagaa
     6781 aaattacttg aaacatcaaa agaagattta tatctagaca atggatatgt aattagtaaa
     6841 cttgatgaaa atgtaaaagc atcactttct gatgtcgcag tatatagtca aagtaaatca
     6901 ttaagagagt taactgttgt tgaagattat tcttcaccag ctggacttac ttcttatggt
     6961 gtacattttg ctgaagtttt agtagataaa gaaactaagg atataaaagt agttgatttt
     7021 gtagctgtac atgatgttgg tagagttata aatcctttaa atttagaagg tcaattagaa
     7081 ggtgggattc atatgggtct aggatacgca ttgagtgaag aattggcatt tgatgaacaa
     7141 ggtagaccta aagctactga ttttaaaaca tataaattat ttagaactgt agatatgcca
     7201 aaatgtcaag ttgcatttgt aaatagttat gaaaaagctg gaccttttgg tggtaagagt
     7261 attggagaat gtgctgttgt accagttgcg ccagctgttg caaatgcagt atataatgca
     7321 actgatgtag aaatacattc attgccaata aaattaaaat agtagaaatt aaagcacaaa
     7381 caacaattta ttgtatttgt aaatagataa taaaaataga tttatttggt gaggagatat
     7441 tattatggca aagatattag gtatttgtgg aagtccacgt aaaggagcaa cagaatatgc
     7501 agtgttagaa gctttgaaag aagcagaaac tatacctggt atagaaactg agtattggtc
     7561 agttagaggg aaaaaaataa gtccttgtgt acattgtgat gcatgtataa gaaaagaaac
     7621 tatgtgtatt ataaaagatg atatccaaga attagagcaa aaaatattgg aggcagatgg
     7681 aattatagta ggttctccag tttatgatat gaatataact gctcaactta cagcagtatt
     7741 taatagactt cgtccaatgt atttagttca tccaggaaaa cttcaaaata aggttggtgc
     7801 agctataact actggtggaa ctagatatgg tggccaagaa ttgacaaaac ttcctataat
     7861 aaatttcttc ctaatgcatg aaatgttagt atctggaggt ttaggtggat gctatatagg
     7921 tggtacaata tggagtaaag atggaaaagc aaaaggtgcc caagaggatg agacaggtat
     7981 ggacacagta aaaagacttg gaaaaggtgc tgcagaggca gttatggttt ctaagtttgg
     8041 tcttgaaaaa tggaatgtcg taaaagaaga attagatgtt aaaaaagatg aaaaatcacc
     8101 tcttagagac cactaaagat ttattatatt attaaatata tacaaatagt gatgtaatag
     8161 attattgcat cactatttta tgttttaatt tagattttat aaattttata ttaaaaaata
     8221 tagaaacaaa gtgttttgag atttattttt aaattttaaa aatgagaatt aaaaagtgag
     8281 aagatagaaa aggtttttta gttattaaat tctacataca ttgtatgaac taaagataga
     8341 aaagaaagaa taataaaagc attgcaattt ctatactaaa gagtcataga aaaatatcta
     8401 gtatatattt agtgttggca tgttaattgc ttatatatta aagtataaaa atttaacaat
     8461 aatttggagg tattttagat gttaaaagaa attaaatgga aagtaaataa tttgccaaag
     8521 ggtgataagg aaaattgtat caaattttta aatgaagaag aaataactaa ggttagaaat
     8581 tttcacaaaa gctttcctca atataaagaa actccattag caaacctaga aggtttagca
     8641 aaaaaattag gtgttgcagg agtatatgta aaagatgaat cttatagatt tggattaaat
     8701 gcatttaaag tattaggtgg ttcttattct atgggtagat acttagctca aagattagat
     8761 acagatatat ctgagttagg gtatgacaaa ttaacttcga aagaaataaa agaaaaatta
     8821 ggagaaataa ctttctttac agctacagat ggaaatcacg gtagaggagt tgcttggaca
     8881 gctaataaat taggtcaaaa atctgttgta ttaatgccaa aaggttcttc tgagtttaga
     8941 ttaaataaaa ttaaaggaga aggagcagat gctagtataa cagacttaaa ctatgatgat
     9001 gcagtaagat tagctaatga ttatgcagaa gctgatgatc atggagtaat ggttcaagat
     9061 acagcatggg atggatatga agaaattcca gcatggataa tgcaaggata tggaactatg
     9121 gctcaagaag cgatagagca attaaaagaa tatggagtag atagaccaac acatgtattt
     9181 gtgcaagcag gggttggttc acttgctgga gcagttcaag gatatgttgc ttcaatatac
     9241 gatgaatgcc caataacagt agtagtagaa gctgatgaag ctgattgcta ctataaatca
     9301 gcagaagcag gagatggaaa accaagattt gttggaggag atatgccaac tataatggct
     9361 ggtcttgctt gtggagaacc aaatactata ggatttgaag tattaaaaaa tcatgctgca
     9421 gcatttgttt cagcaccaga ctgggtttct gctaaaggta tgagaacatt aggtaatcct
     9481 ttaaatggtg atgaaaaggt aatttctgga gaatctggag cagttacaac tggtctttta
     9541 gtagcagcta tggaaagaga agatttagca gatttaagaa aagatttaaa attagatgaa
     9601 aattcaagaa tacttttaat aagcacagaa ggagatactg accctgacaa atatagaagt
     9661 atagtttggg atggagaata tccaagtata taaggaatta ctaaaatgca attattagat
     9721 attggtttac atatttagaa gatatataag ttctaaaatt tagaaaatat ataagtatat
     9781 aaaatacata cattataaaa aatctagggg gataatgaaa tgttaaacga acaaagaaaa
     9841 caagaagtaa ctgaaatatg ccaaaaatta attcaaaatc ctagttcttc tggaaatgaa
     9901 gaaggtgttg taaaagcaat agaagagtct tttgaaaaat taggttttga tagttggtca
     9961 agagatagat atggaaatat agtaggatgt ataaaaggta acaagccagg taagaaaata
    10021 ttatttgatg gacatataga tacagttcca gttccagatg caagtaaatg gagtgttcct
    10081 ccttttgaag gaaaaatagt tgatggaaag atatatggta gaggaacttc tgacatgaaa
    10141 ggtcaatata ctgcaatgat gtctgctgta gcgtattttg ctaaagacac taatcgagat
    10201 tttgctggag aactatatgt agcaggtgtt gttcatgaag aaatatttga aggtgtttcg
    10261 gctagagaga taagtaaggc tgttcaacct gactatgttg taataggtga gtcttcagag
    10321 ttaaacttaa agattggtca gagaggtaga ggagaaatag tagtagaaac ttttggtaaa
    10381 ccagctcact ctgctaatcc agaaaaaggt gtaaatgcag tttataaaat ggctaatgta
    10441 atccaaaaga tacaacaatt agtacctcca actcatccag ttttaggtga tggaatatta
    10501 gttttaacag atataaaatc ttctccatat ccaggggcat cagtagtgcc agattattgc
    10561 aaagctactt ttgatagaag attattagtt ggagaaacaa gagaaggcgt attagctcca
    10621 atacaagctt tattagatga aatgatgaaa gaagacccag aattaaatgc gaaagtttct
    10681 tatgcggttg aaaaagctga ttgctataca ggaaatacta tagaaagtga aagattcttc
    10741 ccaggttggt tatatgacga agaagatgaa tttgttcaag cagcatataa gggattaaaa
    10801 gaagctggaa tagattcaga aataactcaa tactcattct gtacgaatgg aagtcactat
    10861 gcaggtgaag ctggaataaa aactatagga tttggtcctt ctaaggaaaa tcttgctcac
    10921 acaatagatg agtatataga gcaagaacag ttatttatag gaacagaagg ttactatgga
    10981 atattaaaat ctgtatacgg aaaataatat ttagttcaca ataaattatt taaatatagt
    11041 ctagaggtgc tgaatatagc acctttagaa aatacaattt taaataaagt tttacgatac
    11101 ttagaaaata ctttaaagct taaaagttag gaggtatata tgggaacaat actaagaggt
    11161 ggaactataa ttacctctga taagacttat ataagtgatt taaggataga agatgaaaag
    11221 atagttgaaa taggaaataa cttagaaata aatggtgata aggttattga tgctacaggt
    11281 aaaattgtaa ttcctggtgg aatagacaca catactcatt ttgatatgaa tgcaggttct
    11341 attacaactg cagataattt tgaaacagga acaagagcag ctatagctgg aggaactact
    11401 acaatactag attttgcaga agccaatgag ggggaaaacc tactgcaagg tgtagaagca
    11461 tatcataaaa aagcaagtgg aaattgttat tgtgattatg gttttcatat gacaattaca
    11521 tgtttaaata aagatacatt taatcatatg gaaaaactta tttcggatgg gatagtatct
    11581 tttaagatgt atatggcata tgatggcatg aaagtagatg atggaacaat atacagggtt
    11641 ttaaagaaag caagggattt aggttgtata gttggatttc attgtgaaaa tggggattta
    11701 ctagatgtat tgatagaaga aaatatacaa aatggtaatt tagagccaaa atatcatcca
    11761 cttacaagac ctaacatagt tgagaaagag tctgtttcaa gacttgctga tataactaaa
    11821 ttatctaatt caaaatccta tgtagtccac ttaagttgta gggagtcact tgaaactgta
    11881 aaaatggcta gagaaaaaaa tatagatatg atagtagaaa catgcccaca gtatctatta
    11941 ttagaagaca atttatataa taagaataaa tttgagggtg ctaagtatgt aatgtctcct
    12001 cctttaagaa aaaaagaaga cataaattac ctttgggaag gattgtctag tggagatatt
    12061 caaacagttg gcacagacca ctgttcattt aattttaagg ggcaaaaaga cttgggaatt
    12121 gatgatttta gtaagatacc aaatggagca cctggtgttg aacataggtt agcactttta
    12181 tatacatatg gggtcttaga aaatagaata agtgtaaata agtttgtgga agttacttct
    12241 acgaatgctg caaaaatatt tggaatgtat cctaaaaaag gtgagatagc tgtaggaagt
    12301 gatgcagata tagtcatttt aaatatcaat aaagaagaaa caattagtta taaaactcaa
    12361 aaacaaaatg tagattatac tccatatgaa ggatttaagg ttaaatgtaa agttgaagat
    12421 gtatttttga gaggaaatca tgtagtacaa agttgtaatg ttaaagaaca tccaacaggt
    12481 caatatatta aaagaaaaat tactaaataa tgcgggggga aataagaatg atggatatac
    12541 ttattaagaa tgccataata gtaactgtga ataaagagag agaagttatt tttgatgggg
    12601 cattagtagt aaaagataac aagattgctg atattggaaa ttctaaagag atagaaagta
    12661 aatatactga tgttaaaaaa attatagatg ctaaaggtaa agttttattc ccaggtttta
    12721 taaatacaca taatcattta ttccaaactt tattaaaagg attgggagat gatatggtac
    12781 ttaaagactg gttagaaact atgacatttc cagcagctaa ctatttagag ccaaaagata
    12841 cttatgatgc tgcaatgctt ggatgtattg aaggacttag aagtggtata acgaccatgg
    12901 ttgactacat gtatcctcat agtaagccag gtctttgtga tggaataata gatgcttata
    12961 aagaattagg aataagagga atacttggaa gaggatgtat gaatacagga gctcaatttg
    13021 gagttcatcc tggaataatg caagatgtag aaacggttga aaaagatgta agaagattgt
    13081 ttgaaaaaca ccataataca gaaaatggaa gaatcaaaat aggagtagct cctgcagcaa
    13141 tatggtcaaa ttctcaagaa atgcttgaaa tgctatggag agtggtaaaa gaatatgatg
    13201 atgctttatt tacagtacat atatcagaga ctccttttga tagagaagca gcaaaagaat
    13261 tgcatgggca atatgacata gatgttttag aaaaattagg aatacttgga ccaaatgtgc
    13321 ttatggtaca ttgtgtgtat ctaactgaaa aagatatgga acttactaaa aaatacgata
    13381 tgaaagtgtc tcataataca gcaagtaata tgtacttatc ttcaggtgtt gcaccagtgc
    13441 cagaaatgct taaaaaagga ataacagtta gtttaggagt agatggagcc gctagtaata
    13501 actctcaaga tatgcttgaa cttatgaaat taacagcact tcaacataaa gttaataagt
    13561 gtgacccatt agcaatgtcg gcagaaaaag ttcttgagtt ggcaactata gatggagcaa
    13621 gagctattgg aatggaagat gagataggtt cattggagat aggtaaaaaa gcagacttac
    13681 taatatttaa tccaatgctt tcaccaaaag cgatacctat gcataatccg gtatctacat
    13741 tagtatattc ttctagtatg aaaaatatag agagtgttat agtagatgga aatataataa
    13801 tggaagattc aaaaatattg acagctaatg aagagaaagc attaaaagat gctcaagata
    13861 cagctgagag actttgtgta agaggaacta taaaaaatag aatggaaggt cataaatgga
    13921 atagccttta ttaataaatg taatttattc atagaaaagt atagtaaaac tttagcttaa
    13981 aaatatgagt tttgaggtga tttatttgat tatcaaatgt accataaata ataaagaaaa
    14041 agaattaagt ataagaccag atgatttttt agcagataca ttaagagaaa atggatatgt
    14101 aagtgttaaa aaagggtgtg atacaggctc atgtgggtta tgtacagtga tggtaaatga
    14161 caaggccgtg ctttcatgta attatcttac tgctagagta gatggtcagc atattacaac
    14221 acttgagggg gtcttggatg aagcaaagaa gtttggagat tttcttgcag acgaaggggc
    14281 tgaccagtgt ggattttgtg ctcctggatt tataatgatg gttttggcaa tgaaaagaga
    14341 gcttcaaaat ccaacagaag aagaagttat agattatgta aatggtaacc tttgtagatg
    14401 tacaggatat aaaggtcaat taagagcaat acataaatat ttaaacactt agacttttaa
    14461 gtgaaaatta agttgatatg gggagagagt atgataacta taaaggaata tgtagttcca
    14521 aaaagtttag atgaagctta tgaactgcta atatcaagaa aaaacaacat aatacttgga
    14581 ggatgtggat ttttaaaact aggtagtaaa aatattggct cagcaataga tttaaaagac
    14641 ttagctttag attatataaa tgaaactgat gatagcatat taattggagc agacacaagt
    14701 ttaagaactt tagaattaaa taaggttatt aaaaattact gtaatggggt aatttcaaac
    14761 gctgtttcta atatagtagg agttcaattt agaagtggag ccagagtagg agctagcgta
    14821 tttgctaagt atggattttc agatttaata ccttcattac tagtggtaga tgcaaaggtt
    14881 aagttatata aaaaaggggt tatgaactta gctgattttt tagaaagtga gcttgaaaaa
    14941 gatatattaa tagaagttat attacctaaa aaagatgcta taggtgtatt tgattctata
    15001 agaaaatgta caggtgattt tgcagtatta aatggagcaa tgttaaaaga aggaagtatt
    15061 tataaaattg cgataggcgc tagaccaaga agagcaaaaa ttgcctataa agcaagtgaa
    15121 atattaagtt tagaaaatga tatagaaaaa gctggtgaag tatcaagtga agaactttca
    15181 tttggaagta atataagagg aagtaaagaa tacagaaaag atatggcaaa agccttagta
    15241 gtgagaatgt ataatagtat tggaggtgag tgtgatggta aataaagagg taagaaaaat
    15301 agatagtgaa gccattgtta ctggaaaacc aatatataca gaagatttaa tttttcataa
    15361 agatgtttta acaattaagc ttcttagaag tccgcatgct tttgcgaaga taaaaaacat
    15421 agatacaaaa aatgcattaa aggtaaaagg tgtagtaaac atattcactt acaaagatgt
    15481 tccaaattat agatattcaa tggttggaga gtcatatcca gaagcatcac ctcatgacca
    15541 attaatactt gaagacgtag ttagatatgt aggagatgaa gttgctataa tagccgctga
    15601 agatgaaagg gctgctatta aagctatgaa acttataaaa gttgaatatg aagtaatgac
    15661 tcctatattg gatgcaagag aatcggaagg aaataaaatt ttaatccatg aagaagatga
    15721 tttgttttgt ccatttgatt ttggattgga tgctacaaaa aatattgtat cgagagaaaa
    15781 aatgggtaaa ggggatgtag aaaaagagtt taaagaaagt gatgtaatag tagaacattc
    15841 ttatatgact caatcacaag cccattgtat gatggaaact catagagctt atacgtatat
    15901 agatacgaat ggcagattag tagtaacttc tgcaaaccaa tcagcttatc atatgagaag
    15961 acaggtttct aaagcactgg gaatacctct ttcaaaagtt agagtaataa aaccaagaat
    16021 aggtggaggt tttggtggaa aaaacatagc tgtaacagag atatatgcat catttgtaac
    16081 ttggatgaca aaaagaccag caaaactaat ttatactaga gaagaaactt ttgcaatgac
    16141 taatactaga catcagatgt tttttgatat aaaaattggg gcagataaag aaggaaacat
    16201 aaaagctata gatatgaaag cattgaataa tacaggcgct tatggtgata atggaccttc
    16261 agtttgctct gaatcaggtc ataatgtact tccaacatat aataatgtac ctgcaataaa
    16321 atttgatggg agaactgtat atactaattt agttccagga ggagcattaa gaggatatgg
    16381 agcaacacaa ggtacatttg cacttgattg tgcaattgat gaattagcgc ataaacttaa
    16441 tatggaccca acagaaataa agttaaagaa tattataaaa aaacaaactg aaggcggaat
    16501 aatgaatttt ccaataagaa gttgtgcact tgatgaatgt ataattagag gtaaaaaact
    16561 tattggatgg gatgaaaagt accctgttag ggatattgga aacaataagg tgagggctgt
    16621 tggaatgtct gtgggaactc atggttctgg tatagccaac atagacatgg caatagtaaa
    16681 tatgaggatg gatgaagatg gaacttataa gttgttttct gggtcttcag acttaggaac
    16741 tggttctgac acaatacttg cacaaatagc tgcaaaagca ttgaatacat ctattgatag
    16801 aatttccgtt tatacaggag atacagatat gtgtccatat gatactggtg catacgcttc
    16861 ttgtactact tatgtaactg gaaatgctac aataaaatct gctgaaagtt taaaacaaaa
    16921 gatattaaaa gtagcacaga agaaactcga tttaccaata gatgatttaa tactatatga
    16981 agacagagtt tgttatagtg aagatgtaga gaagtttgtt ttgttaagtg aattaggaaa
    17041 agaatctgtt ggtggtggaa atcaagaagt tttatattca tctgaatctt atggtataca
    17101 agagagtgct aaaccattct tagcaggatt tgctgaaata gagttagata aaacaacagg
    17161 ggagtttaaa gtaataaatt atgcttgttc tacagattgt ggtacagtta tgaatcctgc
    17221 acttgctaga atacaagttg aaggtggtgt aactcaaggt atagggcttg caatgtatga
    17281 agaccctaga catggttctg atggaaagct tcaaacttct aatctattac aatacaaagt
    17341 tccaacaaaa aaagatatag gaaacataat tgttgatttt gtagataatt atgagccaac
    17401 aggtccatat ggagctaaat ctttaggaga aatagttatt catacacctg caccagcaat
    17461 agcaaatgca atatacaatg ccacaggagt tcgtataaga gaacttccta taacttctga
    17521 gaaagtttat atggccatga aaaaattaaa tcagggaggt aaataaatga ttgctattaa
    17581 aagtaaatac ttaatatctt ctgctgatga aatatataca aattctgctg ctgtagtaga
    17641 aaatgacatt ataaaagata tattgccaaa tgaagaagtt gaaataaaat ataaaaatat
    17701 tgaagaaata atagataaat cagatgctat aataatgcca ggattcgtca atggtcatat
    17761 gcatcaatat ggagttcttt ctaggggaat accggcaaat gtacatttta ctgactttga
    17821 aggtttttta aatgactatt ggtggccatt tatagaaaat agaattggat tgaaagaagt
    17881 gaaggcaaca actaaagcat cagcaataga acttatagaa tctggtgtgg tagctttttg
    17941 tgatacccta gaagctccaa atacagaaga aggaacttta attgagcaag gaaaaatact
    18001 agaagaaata ggcatgaaag cgattttatc cttagaaagt tgtgaaagaa taagctttga
    18061 aaatggactt agatgtttag atgaaaattc taatttaata aagtggagta gagaaaatag
    18121 taaattaata aatggaataa tgtgtacaca tacaagcttc acatgttctg atagatttat
    18181 taagaaagct aaagaagatg caaaaaaatt aaatgcacca atgcaatttc atttatgtga
    18241 gagtatatat gagccaaact atgctgaaaa acattttggc aaaaaagcag ttgactacta
    18301 taatgattta gatatattgg atgaaactgt attagcatct cagtgtgtaa aagtaaatga
    18361 tgaagaaata gatatattaa aagaaaaagg tgtaaaagtg gttcatatgc ctcttagcaa
    18421 ctgtgaagta ggtggaggtt tttctccagt accaaaaatg attaaaaaag gcataaaagt
    18481 ggcactgggt actgatggat atataaatga tttttttgaa gttatgagag gtgcattttt
    18541 aatgcataaa tcagttgaag aagatgcatc agtaatgcca gcaaatttag tatttaggat
    18601 ggcaacagag catggtgctt atgtattagg acttcaaaat tctggtaaaa tagcagttgg
    18661 taataaggct gatataatag ttatggaaga tgaatttaaa actcctgtga ctcttgataa
    18721 tatatttgac cagatagtag ttcagggtaa aaaagaattt atatcaaatg tatacataga
    18781 tggtagacat attttaaaag aaaaacagtt agttgattta gataaaaaag ctattgttaa
    18841 agaaatgaaa gaagttgctt gtgaattttg gaaattttaa cgaatgtaaa tatatatgaa
    18901 aaggggaagc agataatgta taatttttta ggtaaagaat taaaatcacc acttataata
    18961 ggttcaggac cattaacata tagtgcagct ggatgcaaga tattaagtga tgcaggagca
    19021 ggggcagttg tgactaagac tataagaaaa gaaagagcta taaatccagc accacatatg
    19081 gttagaaata cagctaatgc tcttttaaat aatgaaaaat ggacagattt tgagccagag
    19141 caatggatag attttgaaat accacaaatg aaaagagatg ggactgtctg tatagcttca
    19201 gtaggtcata caattgaaga aagtagcgaa ttagtagaga aagttgcaaa tgcaggagct
    19261 gactttattg aattagtatc ttatgactat agagatttga taccaatgct taaagatgca
    19321 aaggaaagag taaatatacc tgtaatagtt aaacttcctc ctatgataga tgagataggc
    19381 gattttgcta aaaaattaga agaagcagga gcagatgcaa taactgcttg tgactctgta
    19441 ggaccagcat ttagaataga catagaaaca gggcaacctt tattaggtgg aaatggaata
    19501 ggatatttaa gtggagaaac tattaagcca ataactttac agagaatata tgaaataaaa
    19561 aaacaagtta atataccaat aattggctta ggtggatgtg taagtggaga tgatgcctta
    19621 gaaatgatta tggcaggtgc tgactttgtt ggtatatgtt cagtagtaat tttaaaaggc
    19681 gcacaagtaa tatctaaaat acatgatgat ttaaaatcta atcttaatag attaggatat
    19741 aatactatag aaaatgcttg tggtatagta cataatcata tgggagatgt taatgaaaga
    19801 cttagctttg agtttactta tgatgaagaa aaatgtacta aatgtaagat gtgcgagaga
    19861 gtttgtgcat atcaagcgag aaaattgaat gataagatgg aattgaatga agaagagtgt
    19921 agatattgtg gactttgtat atcagtttgt aaaccaaaag cacttggaag aaaaatttca
    19981 tgttcagagc tgaatgtata gattatttta taaaaaacaa agttttaaat aaaaataaga
    20041 aaggctatct caaagagata agcctttttt gttgttgacc ttatttttgt ttatttgtag
    20101 ttattttaga tattaattag ttttgaagta ataaagtgaa tcaattttgt ttaattaaaa
    20161 ttttaataat tctgatatat atgttaacat tgtatagcat ttgtgtaaaa tgttataata
    20221 attaataggg atggtgatgc aagtgcaaga gaagatttta gagttattat atactataaa
    20281 tgaagatgta gttagcataa aaaatgatat gtcggaagta aaacaagaaa tgactgtaat
    20341 gaaagaagat atatcagaag taaaacaaga aatgactgta atgaaagaag atatgtcgga
    20401 agtaaaacag gagataaatg tattaaaagg agatatgtcg gaagtaaaac aagaaatgac
    20461 tgtaatgaaa gaagatatgt cggaagtaaa acaggaaata aatgtattaa aagaagatat
    20521 gtcggaagta aaacaggaaa tgattgtaat gaaagaagat atgtcggaag taaaacagga
    20581 aatgactgta atgaaagaag atatgtcgga agtaaaacag gaaatgactg taatgaaaga
    20641 agatatgtca gaggtaagac aagagataaa tataatgaaa gaggatatat ccgaagtaag
    20701 acaagaaatg actgtaatga aagaagatat gtcggaagta aaacaggaga taaatgtatt
    20761 aaaagaagat atgtccgaag taagacaaga gataaatata atgaaagaag atatgtctga
    20821 agtaagacaa gaaatgactg taatgaaaga agatacatca gaggtaaaac aagagataaa
    20881 tgtaatgaaa aaagacatgt cagaagtaag acaagaaata actgtaatga aagaagatgt
    20941 ttccgaagtt aaatcagaag taaactttat gcaaaataaa ataaataaca ttaatgaaga
    21001 tatgaatgga attaaggacg aggtatccat agcaaatgaa aaattatatg gaattgaaat
    21061 taagattgat agtctagaat gtgaagataa atcaatgaaa gagatacaga tagaacagaa
    21121 taatatttta ggaagtttat tacataattc tgaaataaat aaagctactc atgataatat
    21181 agaacataac atagcatata taaaaggtga cactaattct ataaaagagg atatagctga
    21241 aataagaaga gacttaaatt tagtagagtt agccactagt aaaaattgga gtgatattgt
    21301 caaactaaaa tcagtaaagt agtattttaa ttcactaatt tatcatccac aaactaagaa
    21361 ttttatataa aagaatttat agtgttctta ctattta
//
LOCUS       DAGQWR010000073        21140 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.073, whole genome shotgun sequence.
ACCESSION   DAGQWR010000073 DAGQWR010000000
VERSION     DAGQWR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.073"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(23..685)
                     /locus_tag="MIW95_002259"
                     /pseudo
     CDS             complement(23..685)
                     /locus_tag="MIW95_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(872..1381)
                     /locus_tag="MIW95_002260"
     CDS             complement(872..1381)
                     /locus_tag="MIW95_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBY3151583.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLE"
     gene            complement(1456..1869)
                     /locus_tag="MIW95_002261"
     CDS             complement(1456..1869)
                     /locus_tag="MIW95_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBY3151584.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNSDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2306..2965
                     /locus_tag="MIW95_002262"
     CDS             2306..2965
                     /locus_tag="MIW95_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBY3151585.1"
                     /translation="MDEKILELLQEIQGNISNMQGSISGIEGTVNGMQGNINYMQGNI
                     NYMQGTISDMQGTISDMQGSINGIEGTISDMQGSINGIEGTISDMQSNINDIEGTISD
                     MQGSISSMDGTINSMQDSITDMQGEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQ
                     SDVTEIKSDVKINRNTLDTVIKHTSSLVEDTSDIKNDLNNIEIVTSKNWNDIAKLKSV
                     K"
     gene            complement(3572..3751)
                     /locus_tag="MIW95_002263"
     CDS             complement(3572..3751)
                     /locus_tag="MIW95_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY3151586.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(3976..4509)
                     /locus_tag="MIW95_002264"
     CDS             complement(3976..4509)
                     /locus_tag="MIW95_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBY3151587.1"
                     /translation="MQYISYYHSPIGNILLASDNVGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGEEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     HKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(5327..5899)
                     /locus_tag="MIW95_002265"
     CDS             complement(5327..5899)
                     /locus_tag="MIW95_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBY3151588.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGSCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(5961..6224)
                     /locus_tag="MIW95_002266"
     CDS             complement(5961..6224)
                     /locus_tag="MIW95_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBY3151589.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDLYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(6224..6445)
                     /locus_tag="MIW95_002267"
     CDS             complement(6224..6445)
                     /locus_tag="MIW95_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBY3151590.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYSNPKNRVRAKKGGNMY"
     gene            complement(6964..7500)
                     /locus_tag="MIW95_002268"
     CDS             complement(6964..7500)
                     /locus_tag="MIW95_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBY3151591.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENKDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(7518..8831)
                     /locus_tag="MIW95_002269"
     CDS             complement(7518..8831)
                     /locus_tag="MIW95_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBY3151592.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEENIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            9167..9607
                     /locus_tag="MIW95_002270"
     CDS             9167..9607
                     /locus_tag="MIW95_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBY3151593.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLETQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVSLTPKA
                     KELWVTRYTCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(9792..9980)
                     /locus_tag="MIW95_002271"
     CDS             complement(9792..9980)
                     /locus_tag="MIW95_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151594.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            11041..11436
                     /locus_tag="MIW95_002272"
     CDS             11041..11436
                     /locus_tag="MIW95_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151595.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNNIKCEEIKDNVIEEKNNTCTTE"
     gene            11479..13578
                     /locus_tag="MIW95_002273"
     CDS             11479..13578
                     /locus_tag="MIW95_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBY3151596.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEISVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLKKDATEILVPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTGIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNHSIAVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKKDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLREYGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVSGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYY"
     gene            complement(13803..19013)
                     /locus_tag="MIW95_002274"
     CDS             complement(13803..19013)
                     /locus_tag="MIW95_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBY3151597.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRNFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDRQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKTQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLCIPEGIYSINVSGK
                     DAEKLVGEGSIYDGMNGRNGNNAGTSGDILLSFKEIEKRGELKLYVDGGRGGNGQDGK
                     RGDTGITGEAGENAKIGSKKESKEFAKVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKASLLKGAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSAPIDDSDRNYYYFDRYVFNHASNENQVADDGEKLNALYEKHKDWTISKREDSGLV
                     GAEGKDGRSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFIGEKKNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKDELENK
                     MLSVNAKMNLLIQEIIKMKEEEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYAEKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSGSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYIDI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSESIDILKSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIC"
     gene            complement(19028..>21140)
                     /locus_tag="MIW95_002275"
     CDS             complement(19028..>21140)
                     /locus_tag="MIW95_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY3151598.1"
                     /translation="VKQEPCDFSHGRFRPEADILDSRLTKVLDLFQQLNMCLHKDNFR
                     GSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFEELLKDNGKNIHL
                     CKPLESDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIKEIQNNIVSSLED
                     YVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTTLTIERFINVLAKQSGLF
                     LQDSCMELQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEKASKKIMEQNFII
                     IRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSILTNVTVTQNEEL
                     EDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIRALIPNLVISSVL
                     EYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNLDVGSNVKINVSG
                     KSGELVDSSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNGSKGIDGKNGETG
                     DSGSNGADAGYTSSFHGQMFKGYFAKGTSGKKGDKGGAGGNAGRGGNAGNKGNIYFIS
                     DSEEQKNRIINHCDITVKDGEAGPNGRPGSGGYGGSGGYHGYNTLSFSPSGIDKTVYY
                     TGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDDGGFNAPNTKLST
                     AVSKVEENLIKEKYQGYCSEI"
BASE COUNT         7128 a         3754 c         2423 g         7835 t
ORIGIN      
        1 aagtgtgcgt agctattttt taatcaaact ggaatacaga ctccattcct aggaaattgg
       61 ctgaaatata gtctgctgtc aaagatgctg tcatcacaga aattaaaaca gatactgaaa
      121 agtttttatg gacttcttca agtgaaaaca taacacctgc cagtggtgca tgaaaggctg
      181 ctgataatcc agcacttgcc ccacaggtta acaggaattt ttcctctgtc ttccctcggt
      241 caagagctct agaaatacct ttacctgcca tagcaccaag ctgaatggaa ggaccttccc
      301 ttcccaaagc aagaccaccc ataagtgata agaatcctcc aataaatttt gtgggcaaca
      361 ctcgccacca tacttgtttt attttccctg tcatttcccc ctcaagctga gggataccac
      421 ttccagaaat cattggctcc cagtccacca actttgatac aatccacgca agtactgcaa
      481 gtactgcaaa ccaagctatt atttttattg tagaaccttc tacaaatttt aaaatttggc
      541 taagccaagt accagcctgc cctaaaagta tcctgtataa aagcacaatc aaaccagcca
      601 cagccccaac cataagtcct tcaccaatca aaattacctg aaagtgctct gcccgtttga
      661 taatatgaga tgtatccttt ttcatactgt tcctccttca tccaataatt ctacacaatc
      721 tgaacctcca atgactaaag tcatgagtgt gtacagatat tttttaatca acaaaaatag
      781 atttttattt actaaatata agaaaataat tgttcaattt ctcaataaat gtaaatttat
      841 tcttttaggt atttattctc cacagtcgtg cttattccag caaaatattt attagttctt
      901 gctttctctg tgtatagatt ttacgattgt cctcaaactg taatgctaat ttttgtttat
      961 gactatcata gagttttgct ttgtctgtaa aagtatttag ataatctcga aaattgatat
     1021 aattattcca tgcgacacta ttccattcaa caacatgaat atggtgagta cgagtatttt
     1081 tctcaaaatc tcccatagca aaaagtaatt gttttggtac atcctgacct cgaaaaacga
     1141 ttccacattg ctctaacctt tcgaggtata atttagcttc ctctaaagaa gcaacaccta
     1201 caacaatatc aataataggc tttgcgctaa tactgggaat agaagtgctt ccaatgtgtt
     1261 gaatatcaat ggcagtctta tcccatatac ttttcaataa tataatgaca tcttttgcac
     1321 ttttatccca tatcatctga tgttccaata atttaaccat accacatttt aaacctatca
     1381 ctatatacca cctcaatata atccaccttt atatatatat tacaattttt tctatcttga
     1441 taaacagtga tctagctatt tcactttttc ttgacactcg ctacattccc tatcatgaat
     1501 agatataatt cctccacatt tagaacaagt atatctctcc ttctgctttt gcataaactt
     1561 ctctaaaccg tactgctgaa caaatttact attttccata aggcttgctt gatatctctt
     1621 gttataactt ttttcaagat tttttatgag tttacaagga aaattgaaac attcaaaaca
     1681 ataaaacaac cctttttgac agacacaatc ctttatttta cacttacggc agtgttctgg
     1741 ctttcctgta tcgctgttca aacaaccagc acatggcttt ttgtgataac aatgcttata
     1801 gcaaaccata caattcattc cacaaggagc aaacataatc gtatcaatac tctcttttgg
     1861 cattttcata acattttact cctttcaaaa atccaatttg tcgaattgtt atatattcat
     1921 attgtaacat aaaaaatagc taaaaaattc atgcactttc attgccaata aattttcaag
     1981 gaaatatgaa agtttaaaac taagaataaa aagaaataaa aaggaagcta ctaatcaaaa
     2041 tattcaaaca caagtaatca attatgatat taccagcagt tttaaataca aatataacca
     2101 catatgcctc aaaaacactt attatagtat cttagacact tagacaatac acaagattca
     2161 ttaataaaaa agttctcaat atagatatat ttaaccatgt tttgaatagc agataattat
     2221 atgattaaaa attaaatatg acatagcgtt tgtggaatat aacacctaaa ttatgttata
     2281 ataataaaac aggagtgtga tagtgatgga tgaaaaaata ttagaattat tacaggaaat
     2341 acaaggtaat attagtaata tgcaaggtag tatcagtggt atcgaaggta ctgttaatgg
     2401 catgcagggt aatattaact atatgcaggg taatattaac tatatgcaag gtactattag
     2461 cgatatgcaa ggtactatta gcgatatgca aggcagtatc aatggtatcg aaggcactat
     2521 tagcgatatg caaggcagta tcaatggtat cgaaggcact attagcgata tgcaaagcaa
     2581 tatcaatgat atcgaaggta ctattagcga tatgcaaggc agtatcagta gtatggatgg
     2641 cactattaat agtatgcaag acagtattac tgatatgcaa ggtgaaatta aagaagtcaa
     2701 tagtagatta gacaatatgg aacctgatat taaagactta aaaactggac aagttgaaac
     2761 tagtagtaga cttgatacaa ttcagagtga tgttacagaa attaagtcag atgtaaaaat
     2821 taacagaaat acattagata cagtgattaa acatacctct tcattagtag aagatacatc
     2881 agatattaaa aatgatttaa acaatataga gatagtcaca tctaagaatt ggaatgatat
     2941 tgctaaatta aaatcagtta aataatatac catagtaaaa aatacctgaa aatttcacat
     3001 acttctttaa agttatacca aattttatac agaaatactg attatccgac aacacacttt
     3061 acttataaat aatttattaa aaaatactca ttttatccta aaattaacac gactaaatga
     3121 gtcttgatat aataataaaa aataagggag attgaacact aaaaagttgt gtatctccct
     3181 tatattctat aatatagtta tataattttt cagcctaaaa taaaagtcca tctttgatta
     3241 aaacatatcc ccttatcact tataaaagga taaaatcttg ttttcatttt tccatttata
     3301 tcttcttgct taagagtaat aattttttct tttgatactt taagtgtttg gttaaattta
     3361 accaatcgac taagttttgt tcaacatcta tttttcatat tttccaatac ttaatctatt
     3421 ttttataaat aattctttta tttctaactc acttacattt tttacataaa acgaagcata
     3481 taaatttcaa ccttatcttg tatggttctt aatacttaat gagtagctta agtatcaatc
     3541 attataaatt ttccccttat atttaacact cttaccattt ccaaaatata caaaatatat
     3601 tttgaacatt tgtacaatga tgaaatacaa tacatgaaaa tacaatatca taactgttat
     3661 ctaaaaatag aagttcttca acataacctt tcacaaagaa catattatct aagtattttt
     3721 tgtctgctcc ttgttttcct gtttgtagca tataaattac tgcatctaaa catataacta
     3781 ctcacacgaa aaaagaaaat aaacttccaa aatacaagct ctaaaactac ctctaaagtt
     3841 atataatgtt tactaggaat aaggttgtat aacatatagt ttaaagaata gttttttaaa
     3901 attgcaagtt attgctttca aaattagatg tttatatatc aaataaatga ttaagaattt
     3961 ctaaatttaa tggctttata atgcactccc tttctttggg ataaaataat gttgcacaga
     4021 tactttttct aaagataaca attttatttt tctatcaatt ccaccagcat accctgtcaa
     4081 actcccattt gttcctacaa cacgatgaca tggaacaata atagagattc cattatgccc
     4141 tactgcacca ccgattgctt gtgctgacat tttagatatt cctttttgtt gagcaacttt
     4201 atgtgcaatt tctccataag tgattgtttt accatatggt atttgttgta aaattttcca
     4261 cactgtagtc tggaaaggag tacctaccat atggagtgat ggtgtaaaat tgggctcttc
     4321 tcccgaaaaa taaatatcta gccatttctt tgtctgctcg aaaattggca attctttttc
     4381 ttgatgttct tggtcaagat tataagcata atacttttga ttctcaaacc aaagaccagt
     4441 taacccaaca ttatctgatg ctaaaagaat atttccaatt ggactatggt aatagctaat
     4501 atattgcata caattcctcc ttatattctg aaaacttcac tcaaatcatt agacacttct
     4561 tttctatctg ccattatgta aaaacatact aatataattt ccttctgact acactttcgt
     4621 tataatatct atcctcatat catattagtt ttatatttac ctgaatctgt tcatcctttt
     4681 tttcctttcc ctttgatgga tataaaattc atacatttaa gcttcctact gtattaactt
     4741 ttatagaagt gtatattata attttatcac taaatagaca aaaaaatatc gatataacat
     4801 caagaaatat tttactgagt ttcgcttatt aaaagcaact gaatttctaa agggtacaga
     4861 tgagctaata agtgatattg atataaaagt tgtttttcat taagtaatct attttagtaa
     4921 atgttttaaa gaaaaaacgg gatattctcc aaaagtgtac agaaatataa aaaaagagat
     4981 atgatttttt atttcttttg attatccaaa gagtaaataa aaaatattgt aaaaaactta
     5041 ctaacaacgg caatgctttg aactatggta atactttgaa cttttgattg gacttttttt
     5101 gctaaaataa tatatttaaa tagcaaagta atctgtttaa tcaataaaaa ataagagata
     5161 aaagaaatca actaaaacct ttacctctta ttttgatagt ttatcagagc taatatttac
     5221 actcttttta tttttgatac ttcattaaaa atgatgctta ttctcttttc atttttaata
     5281 cttcattaaa attgatgctt actctctatt cacttttaat acttaattag aactgatgct
     5341 tacactctgt aaactcataa acatccatta atgcttcacc aaatctttga tagtgaacta
     5401 cttccctctc acccaaaaat ctcaatatat ctttcaaatc agggtcatca gttaaattta
     5461 ttaattgata gtacgtagcc aatgcttttt gttcagcagc catatcctca tgtaaatcag
     5521 ttaccgaatc gccaaagaca ttaatataac ttgtagtaaa gtttactcca ttagaatcta
     5581 ctggttgaag accatgtcca aaattagcat aattactacc aagtccagca gccttaagct
     5641 cttccggagt acaactgcct attaattgat ataacactga agaaattatc tcaacatggg
     5701 ccatttcctc tgtacctata tcagttaaaa gtgcgcgaga ttttccagtt ggcatagtat
     5761 atctttgttg aagatatcta gttgcagctg ccaactcacc attaggtcct cccaactgag
     5821 tcatgatata ttttgccatt ctagggtctg cttgtcttat gttaactgga tgttccagtg
     5881 ttttttgata aatccacata ttatcttact cctccttaat atatatttaa taataattct
     5941 aattttaaaa cttaataatt ttatttataa aatgatttgt cccatggcca aggttcagat
     6001 atccatgacc aaggatattc acttgattca tatccaaaat tagttaatgc tccatattta
     6061 gattcataat cacacacagc ttgtgtaaat ttatcgcagt atctattgta taaatcaaga
     6121 gctttcttat cccttggatt gttatctaaa tataaattta attctataca ggcaaattga
     6181 tattctgaaa ttctctctaa aagttctctt ctatttaatg acattagtac atgttacccc
     6241 cttttttagc tctaactcta tttttaggat ttgaatatag ccaatcatta tagttatttg
     6301 actctattaa atctaattca ggaaataaag tacctctttt taatgctgtt tttggattgt
     6361 atgtgtcatc ataaatttga cgattgatgt atggtctagc aaattcacaa caatagtctt
     6421 gtttatcctc ttttaaatat cccattaatt atcctcctta atttttagaa ttttaaaata
     6481 gcattatttt taaaaattgt atacttgttc tatattatag tcagtagttt taataggtct
     6541 ttaataagat taaataatta aataagtatc tttttttgtt cttattttac ctataaaaat
     6601 tttcctaatt aatctttcat aaatatctat tttaaataaa taatatagag tttatacaat
     6661 tttttataac ttaattacta tttactatat attattcatt tttaaataaa atgtgattat
     6721 tttttattaa aaacgatata aatatacaat ttaatactat ttttaacaaa taaatttata
     6781 ttaaaataca ttaatttata tagttttatt tattctaaaa gtataattat aaaaataaaa
     6841 aaagaatagg atatttttcc tactcttttt tctgtgctta tatataatat taaagctctt
     6901 tattttcaat atatatttta aattttctct ttttaatata tctttttctt ttaataaatc
     6961 tctttaattt tcttcttcta tatcagagcc aaagggaatc catgcattac caaactcttt
     7021 atctttaaat aaataagcta atccagttat ttgcttcaat ctaagaattt catcaacttc
     7081 catacccagg tgctttgata tccattcatc agattttcca atttcacaaa gttcagttat
     7141 aattttactc attaaatcca cattatgaga acctcttgca cgattgtgtc gaatagtact
     7201 tgccattctt tcatcaagag atttatcaat tactgaaaca ggaagaagtc cgttttctct
     7261 atcatatata tccttatttt caagcattac tctatatcta tgaaaaccat ctacaataat
     7321 atacttatcc ttttcctcat tataataaca gacaattggc attgtatacc catcttcttt
     7381 tatactttta tacaacaact gcatttctgg tggggctact gcatttggat tatagtcgtt
     7441 agattctatc ttttcgattg gcactgcgat gattccatat acaggacttt tatattccat
     7501 agcatcttaa ctcctatcta atgaattttt tcatatttct tcttaatata ttccgttcta
     7561 attctctgtt cttttgtagg accaaatccc ataaatctac acatatggtc attttttaaa
     7621 atacaataac acattctttt ccaacttggt atatctttgg taaatttaat atcatccgta
     7681 tcatcaggta tatcatccaa aaatatgact ctactctttt tatcttttgt atgatttgaa
     7741 actccattta attctatttt ataacctttt ctcaacaatt cacgtataac tatttctgac
     7801 aaaccaccac ctgtattctg ccaaaactca atagatgttg aaaatttttt aatatagcta
     7861 ttccttatat tttcaggtaa ggtatcaagc aaaaaatatg tgaaactttt ccatgtgtgt
     7921 ccatatggca atttaacact acgatatccc atagcttttg tttttccata aatagaagta
     7981 aaattaactc cttgtactct agcaaccaat ttcacccata catctgggtc caatattctg
     8041 tataaattta aactctcttt tgctgaatca ttaaaaggtg atgccactct catttttctt
     8101 ggagctactc cagctttata gaataagtca taaattttat tatagtcata atcaaattta
     8161 tagtgggcta accatatatc ttctatttcc caatcatata taggagatgc cgtccagaca
     8221 tttttaaatt ccttagttat ccagcacttt cctttatatc catgctgttt attcacaata
     8281 gaacaataac gttgtagaga ttctgctgct cgtgttccta ataagcaaac aacttttgca
     8341 ccttcatgta tatctctaat ccatctacca aattgctttg ccaaactttc ttgtctcatt
     8401 ttatacttat aataagtaaa aggattattt ttttgattta ttatgtattc tttgactggc
     8461 atttctctta cccataattc ttctttctca tcatcccatg gataccaata catttcataa
     8521 tttcctacag cagttcttgt agccattggt aaacacaccc aatatggttc aatatcttca
     8581 atattctctt caaatacttt ttcaacatat tcagtagtca aactatattg agcctcaaaa
     8641 tcttgatgaa atactcctat tttggaactt atattattct ttctcttata atccaaaact
     8701 aaatttagca gtaagccact atcttttcct ccactaaaag atacatagat ataatcaaat
     8761 tcattaaaaa gataagaaag tctttcttga aaagcttcaa atacatcgga ttctaaatat
     8821 ctcttatcca tttgaattat cccctttcaa ttagaagttt ataaaacctt acaatacaat
     8881 attattatgc ttttttagca gtaatcagca taaattatac attatttaaa tacacaaatc
     8941 caactaaaca catataatta taatgtaatt atacttttgt attataaact atattttatc
     9001 attattttac atttttttcc aatatatata aatatcatat ttttctatta gctaaaaata
     9061 attactttat atttttacat gttagtataa taagacataa aaacttgaca tactgttgtc
     9121 ataattactt tgctataatt aaaagtaaaa atggaggtaa attataatgt atgaacgtat
     9181 gcttgataaa agcaataaac cctgtttgtc tcaaataata gaatatatag gtaaagatgg
     9241 atatgcacga ttgtgtaagt tggaaactta cttggaaact caatataatt tatcaagaga
     9301 acttcgattt ccttttggaa acaattatgg ttggggttac aaatatagcc ataaatcttt
     9361 tcatttattt tatgcttttt ttgaaaaagg tgcatttact gttctgattc agataggtga
     9421 caagcaagtt ccagcagtag aaaaaacgtt ggtatctttg acaccaaaag ctaaagaact
     9481 ctgggtcact cgttatactt gtggcaataa tggaggttgg attaagtatc gtattttaga
     9541 agatgcagac ttacttgata ttattcaata tataagagca aaaaaatctc cacctaaaca
     9601 aaattaactt ttattttttg tgatttaaat atagtgtata taaattccat tctttccata
     9661 ttaaaaaaat aatataaaaa atatctatta gtatatctta tttagtatgt cttattctac
     9721 tttttacaga taattaaaaa gttttatcat aaataaatca taactaaaca tcctagtaaa
     9781 tcaaaattca tttattcttt tttattagaa tttttgataa cagtccctat acatatgcca
     9841 attatcattc caaaaattag accataacac attgtgatac ccacatttcc tttagaaata
     9901 cctatagcac aacctatagc cattccaagt atcatgccta taaacataca tataataact
     9961 aaattgttgt ttttattcat agtttttctc cattcaatat attttaaatt gcctagctcg
    10021 ccttcttagc ttaaaacaaa gaaatataaa ttaactttct ttcattctaa tatctgcttg
    10081 tataaatgtc aaacaacaca tttcttgtca taacctcctc tttttgaagt aagtacaatc
    10141 tcccagacac aataatatca atttcctaaa accatttaag taacttttat atttacataa
    10201 tatttacaat cataaactat attgttattc atttatacat cataaacact ccctcctaat
    10261 cattattata cttaaaataa tgttcattta taataatata tctaacgcac tttaattgaa
    10321 aaaactaaaa ctttgccttt cattcccata tataaataag tatacttttt gatgctctat
    10381 tacaaatcaa ctaaattaat tgatataaat tatcattttc tgttgaaaat accatttaca
    10441 catgctatac ttaattgcat aaatggtaat aaatgtcata tgattttaca aatctattaa
    10501 taacaattat tacttttact gtattttaaa gtttttggac taaatatcaa gaactttcta
    10561 aaatacaaag tacaaaaaat ttatataagt tataaattta tatgaactag ataagctcaa
    10621 acaatatgtt atgtataaac tgtgtagtat tcggttataa atttatataa attatataag
    10681 ctatataaag tattacctca actatataag ctagatatat aaattacata cataagctag
    10741 atatataaat tgcatatata agctatataa gaattatttc aactatataa actacatata
    10801 taaattacat acataagcta gatatataaa ctagatatat aaactgcata tataagctat
    10861 ataaaaatta tttcaactat ataaactaca catgtaaaat tcatgtaaat ttactaatat
    10921 aaatcaaata aaattactaa actctctaaa ctaattttgt aaaacatact ctatttttaa
    10981 catttgttac cttatacata agtataaaca aaatctaact aaatgaaagg ataatgtaaa
    11041 atgaatattt taaaaatgaa aaaaacagga atatttttag gaggagtact ttttggtact
    11101 gtaggtgtta agatactttc aagtgactgt gtaaaaaaaa cttgcactcg tgcaacagca
    11161 ggagcattga aagttaaaga ttctgttctt gaaacaacta cgaaaattca agaaaatgta
    11221 gaagatattt tagctgaagc aaaagaaatc aacgagcaag aagacttatc tacatctaag
    11281 gtaaacgttg tacaagatac tcttaaatgc gaacaagaag ttcttaaaac tgaaactata
    11341 aaaactgtag aagatactaa ttctaataac attaaatgtg aagaaatcaa agataacgta
    11401 attgaagaaa aaaataatac ttgtacaaca gaataaattt aatgattatt atgattaata
    11461 atatcaggag gaagtaaatt gaatttttat ataaaacacg aaataagagg tcgtatacgc
    11521 tttgacttac aacttggcaa attaaacaac aagcagtccg atactctctt atattattta
    11581 acttctataa atggtattac caaagcaaaa gtttacgata gaactggtga tgcagtcatt
    11641 tattacactg gtgaaagaga atacataata agagaaatca ctaaattcag ttttaacaat
    11701 agcaaaatag aaaacctagt tcctgaacat accagcaggg aattaaacaa ttattataga
    11761 gacaaattag caaataaact gctagtcaaa agtatctcca aaatattttt tccaaagtcc
    11821 ttaagatttt ttttaattgg acttcattct ataaaatatt ttaaaaaagg tctctcttcc
    11881 ctactaaata aaaaaataga aatttctgtt ttagatgcca ctgctatagg tatttctatt
    11941 tttagaaaag atatcaatac ggctggttca gttatgtttc ttcttggtat cggtgagtta
    12001 ttagaagaat ggactagaaa aaaatctata gacgacttgg ctcaaagtat gtcactaaac
    12061 attgaaaaag tatggttaaa aaaagatgcc actgaaatac tagttccaat ttcagagata
    12121 aaggaaggag atttagtcag tgtaactatg ggaaatatga ttccattgga tggagttata
    12181 gtatcaggag aagcaatggt caatcaggca tcacttacag gtgaatccct tgcagttaac
    12241 aaaaaagaag gctcttacat atatgctgga actggaatag agcaaggcaa tattgttatg
    12301 tgcgtcaagg aaaaagctgg tactactaga ttccagaaaa tagtaactat gattgaagaa
    12361 tctgaaaaac taaaatcttc tgtagagagt aaatttgaac atctagcaga tacattagtt
    12421 ccatacagct ttttaggaag tatattaacc tatgcaatta ctagaaaccc tataaaatca
    12481 ctctctatat tgatggtaga tttctcttgt gcattaaaac tctctatccc tatttcagta
    12541 ctatcagcca tgagagaatg taacaatcac agcattgctg taaaaggtgg aaaattcctt
    12601 gaaggtgttg catgtgcaga tactattgta tttgataaga caggcacatt aacaaaagct
    12661 caaccaactg tttcagatat aattacattc caaaattata ataagaagga tatgttaaga
    12721 cttgcagctt gcttagaaga acacttccca cactccatag caaatgcagt agtttatgaa
    12781 gctgaaaaac aagggcttag tcacaaagaa atgcatacag aggtagaata tattattgct
    12841 catggcattt caacaaaggt cgatgataaa aaggttgtaa taggtagcaa acattttgta
    12901 tttgaagatg agaagtgtac tatcccagac ggtgaggagg ataagtacaa caatctatca
    12961 gatgagtatt ctcacctatt tatggcaata tcgggtaaat tatcagcagt tatttgcata
    13021 aatgaccctt tacgaaaaga agcaaaatat gtaatttcaa atttaagaga atatggaata
    13081 aaaaagattg tgatgatgac tggagatagc gaaaaaacag ctaaatctat agcaagtaaa
    13141 gttggtgttg atgaatatta ttcagaagtc ttaccagaag acaaagccaa ttttgttaaa
    13201 agtgaaaaac taaaaggtcg caaagtaatt atgattggtg atggaatcaa tgattctcct
    13261 gctatttcag aatcagatgt cggaattgca atgagtgaag gtgctgaaat agcaagagag
    13321 atttcagata ttaccatatc agcagataat ttgaataatt taatcgtttt aaagcagatt
    13381 agtaataagc ttatgaaaag agttgattta agttctaagt ttattatcgg ctttaattta
    13441 ggtctgattt tacttggcgt aagtggattt gttagaccaa gtacatctgc ctttttacac
    13501 aacgcatcta ctgttggaat aagtttaaac agtatgacaa atttattgga aaacaccaat
    13561 acttataact actattaatc cccattatat aaaacatatg tatataaaaa atttataatt
    13621 catcttttaa aaagaaactg tcttaaaagg agcttttttc taatcaaaat tatataaaac
    13681 atattttaca aattttaaac tcaacttatt tctatttttt acaaaaagga gatgattcaa
    13741 attttcattt tgaaacatcc ccttttgtaa atttctttaa aacttatata ttaattatat
    13801 tttcaacaaa taattgtatt ttcaaccaat ctttcttcat agcattttaa atcattttgc
    13861 tttagataat taaattcaga ctctacataa ataccacagc ctaccaattc aattgtcaaa
    13921 ttaggtatat tttcctttag tatactagtc tgctcataaa taattctttc caataaatca
    13981 acttgtaata tagaaaaatg cttcataatc tccccatcac tatctatatc tttcttgatt
    14041 tcataccaac tctttgacaa aattcccttc aaatcaatat accctagact cttcaatata
    14101 tctatactct cgctccatgc atacttacta ggtttatcaa agtatgattc tagtttcttc
    14161 ttctgctcat ttgtaagtga atcataagaa agcttcttat taaaatgtgc cttcaaagaa
    14221 atcttagcca tagaatatgg acttctacca gagtttaatc ctgcattttt gaacttttca
    14281 attcctttat cttctactga agaccatatt gagtttattg aatctgaatc attgtattta
    14341 acatcaagac catgtgtaat atctatatag ttttgtggaa atacatattg ataatagaca
    14401 tcttttttat catcatatag ggagaagtat ccactatcat ataaatgaat atgcaccttt
    14461 acttttaaac tatcctctaa aatatttcgt ctaaggttcg ggatatttgc atacatggtt
    14521 acatatttta tggcatcaaa gtttttattt aatggaactt caaaactaat ttcatctcct
    14581 gtcaaaagct ttttcacttc ttcttcatta caatcatata gagaatatgt attttcaaat
    14641 cccacacgtg atttcccatc taaactgcct gttaatgcta ccttcaaaat atggttatcc
    14701 gttttttgcc attggtaagc atcttggtca agaaactctc taatctgtct attttgctct
    14761 ttcgcctgat ttataacctc gttttcatct tctgtaatac ctacatggca tctatttaag
    14821 atatttacta tattgcttcc aaatggaaac gtccatagtt gaaaagctgc tgcctcaacc
    14881 tgacacaaat atctaacctg atttaattca atttgatgga taagtttttt ctgatattca
    14941 ttcatctgat taccactacc tgacatttga taaatcaatt ctcccattgc aagattatct
    15001 tttttcttat caatcttatc agtaatgata atttgagttt ccatcatatc ctgtactttt
    15061 ttaaatatta tagaaatctc ctctgaattt tccattttta agcttccaaa ctttgtgctt
    15121 aattcagtta aaacttcctt catctttaat ttacttatct cattttcaaa aatatcttga
    15181 tttttaactg tactatcgaa tttttctaca aatggtataa cagattcttt caaagtttta
    15241 tctttaaatt ccaccatttg agtattcaat tcatctaatt tcttaatatt attttcttta
    15301 tctttaattg cctctttaac tacctctatt ttactctcaa aactagattt tacattaaga
    15361 caattattaa caccactaat cgcagtattt ataagagaac aagcagaact tccaatactc
    15421 aatgcatttt taattttaga tgtcttttgc tgtctttcaa tagcttgttt acttttctct
    15481 agtgcagcta acttatcttt ttgctgttta ttattgtaat ttgcctccaa catacctatt
    15541 tcttttttct ggatttttgt gtaaatttcc ttatactcgc tttcacttaa cccctctttt
    15601 gctctttgaa tatcgtcagc tttttctgca tagttatcat catctataga cttaactata
    15661 atctccttaa tttggtcaaa tttagttctt ctttcttcat caacacctgc caaattataa
    15721 gtcattttat ctaaagcatt ttttctatct tcacttagct gttctccaag tacaaatctc
    15781 ataccatcag aatccaattt aaaatctgaa aaatcaacca tcctgttagt ccattggtat
    15841 tcctcttctt tagctgaaag attttccttc aaagattttg cactgtcaaa tgcccctaga
    15901 caagcttgac caacaccact aaatgcagtc agtaaactca atgcgctttg tattccagaa
    15961 agaattaact gtgttttcat ttgttcctca agttttgcct gtttcttttt tagttcctct
    16021 atgtcttctt gctcctcttc tttcatcttt ataatttctt gaatcaacag gttcatcttt
    16081 gcattaacag aaagcatttt attctcaagt tcatctttta aaacttctat ttgttgattt
    16141 ccagattcaa tttgagttag gatactttcc ttataagtat tgaaatatcc ttcttttaac
    16201 tcatctttct ttccttgttc caattctctt aaggtcttgt ccagataatc tgtcaaagaa
    16261 cgaatatctg taacacgatt tcctccaatt tcactttctt caatcatctg cacatagaat
    16321 gctgtttttt gttcaagtgc aaataaaatc atactccatt cgctaaaatt cttcttttct
    16381 cctataaaag attctattct atcagactgc atcaatttat ttataaggta ttcatagatt
    16441 tcctttgcct ccttataaaa ttctttcttt atattcatat catccagaga aagcactgct
    16501 ttatttttct tctcaaaact atagtacttc tgaagtattt ctctatttaa ttgcatctct
    16561 attttctcaa ctgcatgaaa aatttcataa atatgtgaaa gttctaaaat atcagcttct
    16621 aaaattccca ctgcacacaa cagtctaaga tttctataac ctttaattcc agaaaaacca
    16681 aatctctcga aaaattcata gaagaaggta tctattgaca aagaatactc ctgctttaac
    16741 aattcctttt cttttaatgc atctccagag cgaccgtctt ttccctctgc tccgactaaa
    16801 cctgaatctt cacgtttgct aattgtccag tccttatgtt tttcataaag agcatttaat
    16861 ttctctccat catctgctac ttggttctca tttgaagcat gattaaacac atatctatca
    16921 aaataatagt aatttctatc actgtcatct attggagcag aggtacctat atatcttatt
    16981 gtttcttcat atatacctct aattccacca agacctcctg caccaccagc tcctccagag
    17041 cctccttttc ctgctagacc atgatttcca tcttttttac ttatagcacc ttttaataaa
    17101 gaagcttttc caaggagata aacctgtcca gacttgctcc caagacctgc atttccacca
    17161 ttacctcctg tgcctccagt tccaccttgt tttccttttt ctgcttttac cacatagtat
    17221 tttgtacctt taaccttagc aaattctttt gactcttttt tagaacctat tttagcattt
    17281 tccccagctt ctccagttat ccctgtatct cctctttttc catcttgtcc atttccacca
    17341 cgtccaccat ctacgtaaag tttaagttct cctctttttt ctatttcttt aaaagataat
    17401 aaaatatcac ctgaagtacc tgcattattg ccatttcttc cattcattcc gtcgtaaata
    17461 gaaccttctc caaccaattt ttctgcatcc ttaccagata cgttgattga ataaatccct
    17521 tctggtatac ataatttttc tgtaagtagt acaatattct tacctgagaa gacttctttc
    17581 tccatattac agtcaatttt caactcacaa tctccataca tatatataga tgacaaaata
    17641 ttaagttgat aaccagatat tattttagtc aaatccgata aattcacttt aggtatagga
    17701 atatatatag aaagtacatt ttctttctca tatgatttta tatatgtaca actttgaatc
    17761 ttctctatcc tatactctgc atcttcaagc tttttggctt cttcttccct atattcttct
    17821 tctgctatcc aatatttgat atcttttaat gtatcttcta aagtattttt tagcaatgat
    17881 tttaacaact cacttagacc aacaatatga gtctcactac agtagctatt taactctaaa
    17941 agaagacgtg tttcttctaa tatattcact ttctgtacgc catcaccata tgaaagttct
    18001 tgtacaaact ccataagctc tttcatatta ttagtcctac tacataaatc tgtatacatc
    18061 ttatctaatt gagtcttttt atcctttaga ctatttatag aagatagaat ggaaatagaa
    18121 tcttccatac cttttttgac actttctata taaggaacta aaagtttttc taggtcttta
    18181 taggttacat caacggcttt ttgtatttct attttacaag aagcagtaag catcaatccc
    18241 aaatctgttt tttcaaatgt ttcaggtaga tttttcattt catccaacaa cttttctggc
    18301 tgtctatcta gtggattaca taatttaata gatacaccat ctgataatgc tacatttaat
    18361 aagtacacta attccttaca accgtcgtaa tctttctctt gactatctag gtcttctttt
    18421 gctttttcca atatattcct tattttttct tctgttacat taccgattag tgttttaggt
    18481 attttattga taagaaagaa gaaattttct ttatactttg cataatcacc aagcatatct
    18541 ttcaaatttt tgcctgtttc tatcaataat gtatgtctag agtccagtaa ggattgatag
    18601 tctaaaataa ctaccatccc tttcaccttt gaagatgtag aaatagtttc ccttacaagc
    18661 atagcaccag caatcatata ctctgctcca cggttatcaa aaaaacctgg gcaatcacat
    18721 aatgaaaaat ttctatttgc aatatctaca acttctggac ataaagtctt agaatataca
    18781 ccatttccaa tttcgacagc tgaatctttt gcagttatat atatttgtcc agtctcttca
    18841 tccgtttttt cttccatcac gcaacctttt aaataattta tacaagtact ctttccaacg
    18901 cctgaagcac ctaagaataa caaaattgac ttttctttta agtcatattt tctaagctcg
    18961 tctcttcctt cttccaaaag gtttacgatt tcactataaa gtttatttgc catacatcca
    19021 tccctttcta tatttcggaa caatagcctt gatatttttc tttaataaga ttctcttcta
    19081 cctttgaaac agctgttgat aacttagtat ttggagcatt aaatcctccg tcgtctcctc
    19141 tatctccata ttcagcgtat tctcttgctg gtcctgtttt gccttcattc caacttccgc
    19201 cactaaattc tatatgatat ttaattatac tatctaactt ataatcccaa cttgtaaaat
    19261 atcctgtata gtaaacagtc ttatctatac cacttggaga aaatgaaagt gtattatatc
    19321 cgtgatagcc accacttcct ccatatccac ctgaacctgg tctaccattt ggacctgctt
    19381 ctccatcttt cactgttata tcacaatgat taattattct atttttttgt tcctctgagt
    19441 ctgaaatgaa gtaaatatta cctttattac cagcatttcc acctcttccg gcatttccac
    19501 cagctccacc tttgtcacct tttttaccac ttgtaccttt tgcaaaatac cctttaaaca
    19561 tctgtccatg gaaactactt gtatatcctg catcagctcc attacttcca gaatctcctg
    19621 tttcaccatt tttaccatct attcctttag aaccatttcc tccatttgca atcagtgtta
    19681 aacgataatt tctaatttct tctttaatat ataagtaaat attacctgca tctctaccat
    19741 cttcaccatt tttcccatac atagaactat ctacaagttc tccgctcttt ccacttacat
    19801 taatttttac attactacct acatcaagat tgttacaagc aagaataata tttttctctg
    19861 atgcaaagtc cattccaagg ttatcatcca tataaatagt atctttggca aagaacataa
    19921 atgtagaaac attgtcatct acttttctat ttttgaaata ttccaataca ctactaatta
    19981 ctaagtttgg aattaatgct cttatttcaa tacctttctt atcattaata ttaattgttt
    20041 ttactataaa tggatttaaa atgtatttac caataaattc tctaattatc tcattacctt
    20101 cttctccata tgcacttaat cttgttatac tgtcttctaa ctcttcattt tgtgtaactg
    20161 taacatttgt caaaattgaa ttaaaatcac ttacactata tacataatcc tttttatttg
    20221 caacttccat taatttttct ctatttggta atgtaaatac ctgtttttgt acttcataag
    20281 aaactaaaac tttttctaaa ttctttctta ttataatgaa gttttgttcc attatcttct
    20341 tacttgcctt ttctataata aattccatat gtacagaagg taataaaatt tccccagttc
    20401 catgttctgt atatttactt aaagcatcaa ttatatctaa gtttcctttt atcttatttt
    20461 gtaattccat gcaactatct tgtaagaata agccactttg tttggctaat acgttgataa
    20521 atctctctat agtaagagtt gttgtacttt ccaaccagtt tttaaatact tttacaacct
    20581 catcatattt tgctatttct tgaatcattg ttatacttcc aataatactg ataaaatctt
    20641 ctgtatacgc cttaacataa tcttctaggc tagatacgat attgttttga atttctttta
    20701 tcaacgtatc taaaactaat cttgcatctg cactaattgg atattggaat gctgatgtct
    20761 tatctttaac ttcatctaat tctagtactt gctttaataa gccttccctc tcgtctgatt
    20821 caagtggctt acaaagatga atatttttac cattatcttt tagcaattct tcaaataacc
    20881 aagctttttc ctctatatta ctatctaaat tttcacataa ttgttcatat tttcttttta
    20941 taacattata tatttgtttt tctgttcttc ctaagaaact ttttgaaatg aaaaaagtta
    21001 tactgcccct aaaattgtct ttatgaagac acatatttaa ctgttgaaac aggtctagta
    21061 cttttgtaag tctagaatct aatatatctg cttctggtct gaacctccca tgactaaagt
    21121 cacagggttc ctgcttcaca
//
LOCUS       DAGQWR010000074        20726 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.074, whole genome shotgun sequence.
ACCESSION   DAGQWR010000074 DAGQWR010000000
VERSION     DAGQWR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.074"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            187..1110
                     /locus_tag="MIW95_002276"
     CDS             187..1110
                     /locus_tag="MIW95_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBY3151599.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESINLTKTKIY
                     SNLRDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDGLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLNKKDEDIYKILSLNLLKIIALGNDNSLKGTKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1085..1912
                     /gene="panB"
                     /locus_tag="MIW95_002277"
     CDS             1085..1912
                     /gene="panB"
                     /locus_tag="MIW95_002277"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBY3151600.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGAKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIADMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGVFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1931..2779
                     /locus_tag="MIW95_002278"
     CDS             1931..2779
                     /locus_tag="MIW95_002278"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBY3151601.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNINTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PKNIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            3190..3372
                     /locus_tag="MIW95_002279"
     CDS             3190..3372
                     /locus_tag="MIW95_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151602.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            complement(3660..3862)
                     /locus_tag="MIW95_002280"
                     /pseudo
     CDS             complement(3660..3862)
                     /locus_tag="MIW95_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003381593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
     gene            4204..5118
                     /locus_tag="MIW95_002281"
     CDS             4204..5118
                     /locus_tag="MIW95_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBY3151603.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKDITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            5508..6155
                     /locus_tag="MIW95_002282"
     CDS             5508..6155
                     /locus_tag="MIW95_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBY3151604.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTAFKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(6322..6891)
                     /locus_tag="MIW95_002283"
     CDS             complement(6322..6891)
                     /locus_tag="MIW95_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151605.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(6896..7795)
                     /locus_tag="MIW95_002284"
     CDS             complement(6896..7795)
                     /locus_tag="MIW95_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY3151606.1"
                     /translation="MKLLSKVNNNIRLIIQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(7806..7955)
                     /locus_tag="MIW95_002285"
     CDS             complement(7806..7955)
                     /locus_tag="MIW95_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151607.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(7948..8532)
                     /locus_tag="MIW95_002286"
     CDS             complement(7948..8532)
                     /locus_tag="MIW95_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBY3151608.1"
                     /translation="MKKFFMVVLGCIFVLGVISGCSKKESKPVEKNKSNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKNIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEEWKKIIDERLKMVKANE"
     gene            8954..10279
                     /gene="cdeA"
                     /locus_tag="MIW95_002287"
     CDS             8954..10279
                     /gene="cdeA"
                     /locus_tag="MIW95_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBY3151609.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GISFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKGRYKY"
     gene            complement(10374..11168)
                     /locus_tag="MIW95_002288"
     CDS             complement(10374..11168)
                     /locus_tag="MIW95_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151610.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(11169..12044)
                     /locus_tag="MIW95_002289"
     CDS             complement(11169..12044)
                     /locus_tag="MIW95_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151611.1"
                     /translation="MRSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(12048..12416)
                     /locus_tag="MIW95_002290"
     CDS             complement(12048..12416)
                     /locus_tag="MIW95_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY3151612.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(12599..13258)
                     /gene="deoC"
                     /locus_tag="MIW95_002291"
     CDS             complement(12599..13258)
                     /gene="deoC"
                     /locus_tag="MIW95_002291"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBY3151613.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(13294..14838)
                     /gene="murJ"
                     /locus_tag="MIW95_002292"
     CDS             complement(13294..14838)
                     /gene="murJ"
                     /locus_tag="MIW95_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBY3151614.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILITGIIFIGITSVMSAFLQIKENFIVVGF
                     GSIPYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKD
                     DSLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYSDRLNGFVTGTFTA
                     SIVSVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFER
                     GAFDARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVL
                     DLVLIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIM
                     GVLSYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKR
                     KLHLKK"
     gene            complement(15312..16196)
                     /locus_tag="MIW95_002293"
     CDS             complement(15312..16196)
                     /locus_tag="MIW95_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY3151615.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(16487..16963)
                     /locus_tag="MIW95_002294"
     CDS             complement(16487..16963)
                     /locus_tag="MIW95_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBY3151616.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGVLDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDKNCGCGCNHHHEN"
     gene            17230..18405
                     /locus_tag="MIW95_002295"
     CDS             17230..18405
                     /locus_tag="MIW95_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBY3151617.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(18486..19289)
                     /gene="proC"
                     /locus_tag="MIW95_002296"
     CDS             complement(18486..19289)
                     /gene="proC"
                     /locus_tag="MIW95_002296"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBY3151618.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINNEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(19329..19913)
                     /locus_tag="MIW95_002297"
     CDS             complement(19329..19913)
                     /locus_tag="MIW95_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3151619.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         7343 a         3139 c         2438 g         7806 t
ORIGIN      
        1 tttatagaat taattgtcaa ttttttatga aaatggtaag atatttatgg atggtatctt
       61 gttataagaa ctatacttta aatgttttta attattttgt cttatgtaac tcatatcctc
      121 aggaatgaga ttagaaatta agatgttgta tcacaaagtg gaggtgatac tattaaaata
      181 ggctttattg gttctggaaa agttggaaca tctttggcaa attatttttt atcaaaaggt
      241 tattgtgttt caggctttta tggaaggaat caattatctt tattagaatc aataaattta
      301 acaaaaacaa agatttacag caatttgaga gatataattt atgaaaacga tatattattt
      361 attacaacat cagatgattc tattgaaatt atagacaaaa aactttccaa atttaattta
      421 aaacataaat acatttgcca cacaagtggc tctttaaaat ctagtatatt gttctgttca
      481 aaaaaagcag gtgcattaat ttattctatc catcctatat ttgcattttc caataaaaat
      541 accaacataa aaaaaatgaa agacatttgt ttttccatag aaggtgataa tgaacaagat
      601 gatttagtta ttcaaaattt catagatggt ttgggaaacc aattttttat aagagataag
      661 gatacttcat ccacttatca tctagctaat gttttagttt caaatttagt attatcttta
      721 ctagatatag gagtaagtta ctttattaaa ttaggtttat ctgaagagga atctttaaaa
      781 gctatcagtc ctttagtaaa aaaaaatata gaaaatatac ttgataatgg ctttactaaa
      841 gctcttactg gtcctgtttc tcgtggagat ataacaccaa taaggaaaca tctatctgta
      901 ttgaataaaa aagatgagga tatctacaaa atactatcct taaacctact aaaaataatt
      961 gccttaggaa atgacaattc attaaaaggt acaaaaaata ttactataga aaatgcaatc
     1021 gaaaatttaa taagtaaatc tgaaaaatac aaagaaattt acaaactttt aggaggaaaa
     1081 aacgatgaaa aataccatac agacttttaa gaacgctaaa tatgaaggaa aaaaactatc
     1141 tatgcttact gcttatgact actcaattgc aaaaataatg gatgaatgtg atataaatgg
     1201 tattttaata ggcgattcat taggaatggt cataaaaggt gaagaaaaca ctctatctgt
     1261 tactatagac gaaattatat atcatacaaa agctgtaaaa aatggtgcaa aaaatgcctt
     1321 aatcgtaagt gatatgcctt ttctatctta ccatgtttct atagaagatg cagttaaaaa
     1381 cgctggtaga cttataaaag aaggaggagc tcatgctgta aagttagaag gtggctcaaa
     1441 cgttataaag caaatagaaa gcattgtaaa tgctcagata cctgtaatgg ggcatttagg
     1501 attaactcct caatcagtaa attcatttgg tgggtttaag gttcaaggaa ataccagtga
     1561 aacagcaaga caacttattg aagatgctaa attaatagaa aaagcaggtg cattctctat
     1621 tgtccttgaa ggagttccta cgaaaatagc tgatatggta actaattcta tttcaattcc
     1681 tacaataggt ataggggcag gaattaactg cgatggacaa attttagttt atcaagatat
     1741 gttgggagtg tttggagatt ttgttcctaa gtttgtaaag cagtatgcaa atataggaga
     1801 tataatgaaa gattcaatta aaaattacat cctagaagtt aatacaggtg catttccaca
     1861 agaaaaacac agcttttcaa taaatgaatc tgagttagaa aaattatact aatattaagg
     1921 atggtacaaa atgcttgtaa aagaaattaa attactaaga aatataatca aagattggag
     1981 aaaacatggc tattctatag ggcttgttac aacaatggga tttttacatg aaggacacca
     2041 aagtcttata aaaaaagctg taaaagaaaa tgataaagta gtagtgagtg tatttgtaaa
     2101 tccaactcaa tttggaccaa atgaagattt taatagctat ccaagagata tagataaaga
     2161 ttttaaatat tgtatggata gtggagcaac agtagtcttt aacccatcac cagaagaaat
     2221 gtatttaaaa ggtaattgta ctaccattaa tgtttctgga cttacagatt ttctttgtgg
     2281 agctaaaaga cctgttcatt ttggaggtgt ctgcctagtt gtttcaaagt ttctaaatat
     2341 agtaacacct gataaagctt attttggaga gaaggatgcc caacaattgg ctgtaattaa
     2401 aagaatggtc aaggatttaa acataaatac agaaataata ggctgtccta taattcgtga
     2461 aaatgatgga ttagcaaaaa gttcgagaaa tacatatctc tctgaggaag aacgaaaatc
     2521 tgctctaata ttaaataaga gcttaagttt agcaaaagaa gaattagtaa aaggcaatct
     2581 aaatccaaag aatataaaag aattaataac agctaaaata aattctgaac acttagcaaa
     2641 aatagactat gttgaaattg tagatagtga gacattacaa cctgttaagc aaatagaaca
     2701 ttctatcctt gtggcaattg ctgtgtttat aggaaaaacc agactaattg ataattttac
     2761 atttgagtta aatatatagt atataatttt atataatata tctaaaaaat aatagaatac
     2821 ctaaaaagta ataaattacc taaaaaataa taaattgctt agaaaataat agattacaat
     2881 aactaaaatt ttaaaattaa attccttaaa agtatgatta attataatta tataagtata
     2941 ttcttattac acttatatta atttatatta aatatctact aactcaaata ttcataatta
     3001 aactttaaac tggataaatt ttcttttaag aatgaaagtt tatccagttt tgcgattata
     3061 ataaccagtt ttacatatcc acttgtaaaa tatatctaag caatatatca tactatatag
     3121 atttatgagt gaaataatat ctgctattta ctgaatattt ttatttttcg ttttttaggg
     3181 ggattagttg tgtttaaaga taagattgat gaatgtgttc atataatgac tgcatatatt
     3241 gctagtttaa aagaatacta ttcattcata gaaactcaaa ttggtgattt tattaaaaaa
     3301 tatggagaag atgttgtaga gttatgtctc catagagtga tgattctact atgcgaatgt
     3361 ggtttggcat aaaattaaat ttattaaaat gggtaagaag aatcctatat aatattcttc
     3421 ttacccattt taatatatca atttcatagc ttatcgttta aaatatttta cctccattac
     3481 tagacaactt tacattctct ttttaattct agtatattta aatcatttta gttgctcctt
     3541 atttattctt attacttgtt ttattttata aataaaataa ttctttttaa taatgttttt
     3601 tgttgatatt cctatcacac atccaattat ttatcattgt cttgaaattg tttgcatact
     3661 taatttaatc caattgaaac accccagcat agaatcccct gtaaaataga agttggatta
     3721 aatcctaaca tccatacaga aaatactact ccaagcacaa gcaatataat ggaatatatt
     3781 tatcacttaa ttttttatac tttttaaatc ccatacctaa aacattaagt gcagctatta
     3841 gaattaataa ttgttgtggt ataaaattat gattgcttca tatttattca ttttttgcta
     3901 taaaagttct tgtttcacaa aaaatatttg ttttgtatca aaataatact tataaattac
     3961 ataattctca taaaaatttc tagtattact tgtgctctta ataaataata gccaacaagt
     4021 accttaaatc attatatcta tatctaataa gaaacacagt aagataattt tttcgcccat
     4081 aacttaataa aaactgttga tattagtagc aattgaattt agttaattta tttataatat
     4141 acataaattt aaatattacg aataaagtat tataaatagg atactttcta aagggaggaa
     4201 attatgatag ataatcaaaa atatgttatt ttatcactag aattacattt atttttttca
     4261 agaattatga aagagcatgc tcttttttta gaagcaggat tcacaaataa aaattataat
     4321 cttgctatgg aagctgacca ctataaaaag caatttgaag atttattatc atacactgtt
     4381 agtgctagta atggtataat tagacctgat atattatatt cagaagagct tgtaactact
     4441 ctcacatcag ttgcagaaca aaaaacagaa gagtttacag ggatagaaat aaacaaagac
     4501 atcactacaa gagaattaaa tctacaaagt ggtgtaaacc cacaagttgg tcaagactta
     4561 gtgaactatg tagctcaact taactctgat gcaataagat tacttgatgg gcttattaat
     4621 tttaaagaaa gagtcttaga tggtgtacta tcatgtacta tatttacatc aaactaccct
     4681 ctacttcttg aacatataat acatgaagca aatttatatc gttcttatgt agttgacctt
     4741 gaaaataaaa tagatattga gtcaaaaaac gctaaagaaa tagaattatt ctgggaccat
     4801 attatgatgg aacatgctct gtttatgaga ggattactag acccctcaga aggtgaacta
     4861 ataaatactt caaatgattt tgctataaaa tttaatgaat taattgaaaa aacaaacgaa
     4921 atgactgatt ctaatatcaa gaacattaca gaagaaactc taaatgaaac tgttgagttt
     4981 aaagatttta aagaagcagg agcatcagga atagaacagt gtaagataaa atctataata
     5041 ttaccacttt tagcagacca tgttttaaga gaggcaaatc attatattag aatattggag
     5101 agttataaaa acatgtaatt aaaaattact tttaaaataa tctttgcata tttagataaa
     5161 ataaaggtac agttaactgt acctttattt tttataaact tataaaattt aaacaagtta
     5221 ttcataaaat aaaataatca tcatctataa cccacaactt tattttatat taaataaaaa
     5281 gtattagtga ttatgtttta taagtatttc tttattcagt tatttgttaa aaactaatag
     5341 taaatcttat actaatcgca tatttatgtt ataactatat tgtaactatt ttcgtaatct
     5401 taggaaaaga ttacttataa aattaacctt atacaataat atatcgtata atcttaatta
     5461 gcataatagt tacaatatta aaatttaaat atgggagtga ttttagtatg ataagacaaa
     5521 caccattaga gaaacgatat gacaagctag gtcctcatat tgtaacagca ttcaaaaaac
     5581 gttattttga tgcttattac tgtaaaacta gaaatgaagc tttgcaacaa attttagatt
     5641 taattcctca aaatcatgta gtatcatggg gaggatctga aacacttaaa gaaatgggcg
     5701 tacaaactgc tgttagagag aaaggattta aagttattga tagagattta gcaaaaactc
     5761 cagaagaaag aactgaaatt atgcgtcaag cacttctatg cgatactttt ttgatgagta
     5821 gcaatgcaat tagtgaagat ggacaactct ttaatattga tggaaatggc aatcgtgtag
     5881 ctgcaatgat atttggtcca aaaaatgtta tagttgtagc tggtatgaat aaaattgtaa
     5941 aaaacattga tgatgcaata caaagaacta gaacaattgc atctccagca gttgttcagc
     6001 gttttcaaga tgtcaataca ccatgctata ttaatggaag ttgtatagat tgcacaagtc
     6061 ctgaaagcat ttgtgcatac atggttacta caagaatcag tcgtccagca aaaaaaataa
     6121 aggtaatatt ggtaggagaa aatcttggtc tataaaatat acatacatgt aaatagctct
     6181 ataaataata aaatcatatg aaataactta tttctaaaag tggtagaata aaagcttcta
     6241 caagactttc attctaccac ttttatattc ttttaatttg tgtttctata ttattaattg
     6301 ctccaatttt catatatatt actaactatt tttattttca taattttttt gttcttcatc
     6361 aagctgttta taataatctt tattatagtc agctagataa tctataccac ctcctgtaat
     6421 cacatttaat aatttcattg cagattcttg attaacttta caaacataat tatcaccaga
     6481 tttaacaagc tctaatgtaa tattaaattc ttttttcttt gcagaatcaa tatctttcat
     6541 agttggattt ggcatcattt ctcctagttt aggaggattc ttttttaaaa tagaaaccat
     6601 atcagtattg ttaattttta aatctacagt cactttatcc ttattattct ttatatctga
     6661 aatttcccaa gtcatatttt tagaatacat tttaaatcct tctttatctt cgtatataag
     6721 attacctaac cctgattttg ggaaatactt atcaacagta tctgcatctt gtgtttttat
     6781 tgaattaaac atactatcca cttcattttt tatcttatta tcattagtac aaccagctaa
     6841 caccagtact gatattgcaa acattataat acatagaata aatttcttca ttaaattata
     6901 tccccctata cttttttgca caaatattat tctctttttt atcttttgaa aaagttgtag
     6961 taattgcatt ttgtggacaa acttttatac attcaccaca cctaatacag tctggactat
     7021 ttacatcttt atatattggt atatcaagct tacattttgt ttgacatgct gtacaatttg
     7081 tacatttatc tttatcaatt ttaaatctat atattgaaat aggattaaaa aaaccataga
     7141 ttgctcctaa agggcatata tacttgcaaa atggcctata agtaatgatt gacatcaata
     7201 atatcaatag caaaatactt aacttccagc taaataaaaa tcctattgat tctcttaaat
     7261 tctcatttcc taaaataagt ggtattcctc ccaaaagtgt tcctgaaggg caaatccatt
     7321 tacaaaacca aggatttcca ccccctgcta catttacaat agcaactggc aaaataatta
     7381 caaacattat taagactata tactttagat attttagata tttatcacca taaacttttt
     7441 ttatctttac tggaaagggt actttatgta gtaaatcttg tacaagtcca aagggacaaa
     7501 gccaaccaca aacaaatctg ccaaaaaaag caccaaatgc cattaaaaaa ccaacaacat
     7561 aaaatgagaa tttatactct ctgcttgata aaactgcttg taatgaacct attggacatg
     7621 ctccaagtgc acctggacat gaataacaat tcaatccagg tacacataat tttttacttt
     7681 ctcctccata tatagttcct tctaaaaatc catttacata tccatttgtt aatgctgtaa
     7741 atgcaacttg aataataagt cttatattat tatttacctt agataataac ttcattcctc
     7801 ttctcctaac caataccaat acactcaaga caaatcataa ttgctttagt taatactgta
     7861 tttgtttctc cacgatatgt acctaatgca ataaatataa tactaattgc aataatagaa
     7921 atgccttttt tattatcttt aataaagtta ctcattggct tttaccattt ttagtctttc
     7981 atctataatt tttttccact cttccttact atttgcacct acaaccttct cacccacaaa
     8041 gtttcctttc ttgtctacaa aataagttgt tggaaactcc ataactcttt taaaatcagt
     8101 ttttttaagt tcttcagata cagtcaattg ttggtaagtt gcatctgact ttttcataat
     8161 gtttttaact aaatttttct cttcatttga aagaccagtt ctcatatcag ttttatcaac
     8221 ttctacaact aatcccttta tagctacatt ggaattatta ctctcatatt cttttgataa
     8281 tgcttcaagc tctggcattt cacccacaca tggtccacac caagtagtcc agacattaat
     8341 aagagtaagt tctttatttg aaaatatctc ttttgttacc ttatttccat caacatcctc
     8401 cgtatctaaa ttactaaacc attcactatt actcttgttt ttttctactg gcttagattc
     8461 ttttttgcta catccagaaa tcacacctaa aacaaaaata catcctaaaa ctaccataaa
     8521 aaactttttc atatctcaca ctcctagaat ttatatagta tataaaatgg ctgcttgaaa
     8581 taaaaataac ttctcttcta tattaaaaca ttatatttaa taaataagaa ctaatttaaa
     8641 ataagttgta taaaagatat atagttaaaa tctatttctt ttcagacaaa atcttatata
     8701 tatataataa cttataaagc ttaagattat atacatatat ttctaaaaat tttcttaaga
     8761 aacagttatt ccttactaat gcaatatacc tatttttact aatataagtt tattaattag
     8821 cattatacta atttatctta atatgatttt ttgttgattt taagctgttt atcgtataaa
     8881 taaaattttt cttgactata tagttactat atagcttatt atagatagag agtaataaaa
     8941 ggaggtaaaa tttatggaaa atttatttac aagaaaattc actacttttg aatttctaaa
     9001 atttgtttct cctgcaatta tatccatgat atttatatct ttgtacacaa taatagatgg
     9061 catctttgta tcaacattag ttggttccga tgctcttgct agtataaata ttgtactacc
     9121 tataattaac cttgtttgtg gatttggaat aatgatggca acgggtggag gtgctatcgt
     9181 ttctatacgt atgggtgaaa atagacagga tgaagccaac tctacatttt cttttatagt
     9241 tttgttttca ttgattgttg ggatttcatt cacagtaatc tcatatttct ttatcaaaga
     9301 aatatctatt ttgcttggtg caacagataa gttattacca tattgtataa cttatggtaa
     9361 agttatgatt ttatgtacac cattttatat tttaaaattt atatttgagt actttgcaag
     9421 aactgatgga aattctaaat ttagtttatt tctatcagtc attggtggtg taacaaatat
     9481 aatattggat tatgtattta ttaaatattt tggaatgggt cttttaggag ctgcagttgc
     9541 aactgctata ggtattattt taacttgtgt tttaggtatt atttacttct tatctaataa
     9601 atctacactc aaattaagaa aaccaaaaac cgattttaga cttataagag atactatgat
     9661 aaacggttct tctgaaatgg ttacagaatt atctacagga attacaacat tcttatttaa
     9721 tgtagtagct ttaaaattag caggagaaaa tggacttgct gctcttacta tagtattgta
     9781 tgctcatttt ttaatgacat cagtctatct aggattcgct gctggagtgt ctccattaat
     9841 aagctataat tttggtgctg aaaacagtga taaattaaaa gaaacattta aacattctct
     9901 aaaatttata tttgtttctt ctcttttagt gtttattatt gctttagtat ttgcaccatt
     9961 tattgttagg gtttttgtaa gtccagataa cacagtattt aaactagcct tacaaggatt
    10021 aaaaatattt gcatttgctt ttttgtttgt tggtataaat atatttgcat caggattttt
    10081 tacagcattt cacaatggaa aaatttcagc tattatatct tttagtcgtg cctttgtttt
    10141 tataatcata ggaatcataa ttcttcctcc tatgttaaac atgactggat tatggcttac
    10201 agttccattt gctgaggtta taaccatatt tatatctatt ctatttataa aaaaatataa
    10261 aggtagatat aagtattaaa aattaatgct taaaatcaac atttattaaa actaataaac
    10321 ctgctttaaa atacaaattt acaaatttga tttttaagca ggttttattt tagttataat
    10381 aagtcatttg catactcttt aattggtgct tttctaagta ataaaattat aaatattatg
    10441 caaataacta taattcctac ctttgttcca aataaaataa aaggattctg aaatgaataa
    10501 ataatttttt ctaaatacaa tagcatttta tgcatatttt tagttgcaaa attaactata
    10561 taaattacca ttaatatcgg taatcctatc aatatagtaa tactagtaac ttttctaact
    10621 ctatatgtaa aagctccaag taatactcct ataaaaccta tacatatttc atttatacat
    10681 atttgtacaa aataatttaa ataagttagt ggcatacttg atgttgaagg atctatcagt
    10741 gctgatatag atgtccaaat catatctctc tttagtaaaa ctgatagttc tatactgtaa
    10801 cctgttataa tttctataaa taacttcgat aaaaaaatca aaactatacc taaaatagaa
    10861 atcacaaagc ttaaaataat taaatctatg acaatagctt ttatacaact ttttcgatct
    10921 gctcttatac ttaaagctcc tgaaaagtct ttatttgcta tatttactcc atacacaaat
    10981 agcagtatag ttatatatgt taaaatagga ttcacaatcg tggataaatt agttttagat
    11041 gcttctccaa aaatcttgag gataaacact ggcaaaatat tcataacaat tcccacaatc
    11101 ataaatataa ttatatacaa tttagtattt ttattaaaaa attttatata tggctttaat
    11161 tcattcaatt acaattcctc cttcttagtc atgttaacaa acatatcttg tagccctatg
    11221 tatcctacat caatatttga atttttaagt attttttcat cattttcact aaaatcacca
    11281 tagtaaaaat aagcaactgc atttccaaat gcctttttag gttttatatt ttttatacat
    11341 tctaattttt ctaactcttc tttatttcca gtaatataat gagctttttg ttttatgaca
    11401 tcaatttctt catctataat aatttttccc tcatttaata ttattacatg ctccagtaaa
    11461 tcatctattt catcaatcaa atgagttgat attattattg tccttggatt ttttatataa
    11521 ctatctaaaa ttatgttata aaactcttgt ctatttactg catccaaacc aattgttggc
    11581 tcatcaaata tggtgatagc tgaattagaa caaataccta ttatatttga aagtatagtt
    11641 ttcatacctc ttgacagttg tttatacttt tttttagtat ttaaatcaaa atatttgcat
    11701 aattcttctt ctaagccttt atcataactt ttataaaaac tagaagaaaa ttcaaatatc
    11761 tgacctactt taaaatctgg actataaaat tctttttccc tgacgataca aacttcttcc
    11821 aaaactttta tatcttcttt tgggttcttt cctaatattt ctatctcacc ttcattttga
    11881 attaactgat ttaccattat atttaataat gttgtttttc cagctccatt cttacctaat
    11941 aaaccatata tcttattttt ttcaaagttt agagacatat tattaaaaat attttgcttc
    12001 ttaaaactgt gagataaatt ttctatctta atagaactcc tcatatatta atcctccttg
    12061 aaattaatta taatatcaac caattcttct ctatttattt ctaatttgtt agcttcttga
    12121 agtaggtttt ttacaaaatt atgtttaaaa ttttcttttc tagcttcttt tataatagtt
    12181 tttgcaccat ctgatacaaa catccctatc cccctcttct tatataaaat atttttatca
    12241 accaatatat ttataccttt taatactgta gctggattaa ttttataaag ctttgaaagc
    12301 tctgttgttg atggaacctg ttcttcttct tttattccat ttgatagtat ctcatcttca
    12361 attgctcgtg ctatctgaat aaatataggt tcttcattat ttaatactaa tttcataggt
    12421 tcctcctttc tccattagtt agttactcaa ctaattaact atataactta taatatcttg
    12481 aaaaatattt tctgtcaata ctaaataaaa aacagaggga aaattccctc tgttttttgt
    12541 tctttatatt tataattaaa ttttgatttt tgtaatccta tcccataagt taaaaacttt
    12601 aataatctgt tgtatcttca ttttttaata ttttacatat tgagctagtt cctaaccttt
    12661 ctgctcctgc ctctataaat ttttctgcat cagaaattga tttaactccc cctgcagctt
    12721 ttatttttac attttttcca acatgctctt tcataagctt tatgtcttct aaagtagctc
    12781 cacctgttcc cattccagta gatgttttta taaaatctga acctgacatt gttactattt
    12841 cacacatttt tattttttca tcttcatcta aataacatgt ctctataatg acttttaata
    12901 ctttatctcc tatagctttt tttatttctt ttatttcttt tcctatatta tcataatctt
    12961 tattttttat atctgaaatg tttacaacca tatcaacttc atctgctcca ttttcaatag
    13021 catcttttgc ttcaaatact tttgtagcag tagtttgata tcccaaaggg aaccctatca
    13081 ctgtacatat ctttatttta ccttttagat attctgatgc tcttttcaca tatgatggtg
    13141 gtatgcaaac tgatgctaca ctcatatcta cagcttcatc acataaaatt ttaatatctt
    13201 cccatgtagt tgtagctttt aaaatagtat ggtctactgt ttttaatata tgtttcataa
    13261 aatcctccta aatttataat taatataatt agattacttt tttaaatgca attttctctt
    13321 tgcaatattc agtatcatat caacctcttc aactttaaaa atagtcatta gtagtgtata
    13381 aattccacct ccaactataa ctgaaatagc tagtgataca aactcattaa atgttcctac
    13441 tcccaaaatt ccaaatataa atttatacgt aaagtatgat aaaacaccca taattaaaga
    13501 tgcaactacc gatttcaaag ttgcttttat tattttatct tgtccaaaat atccaacttt
    13561 tctctttagg ttcaagaaga gtaacaatat acatgcaatt gatgatgaac tagttgcaag
    13621 agcaagacca ccatgagcca ttggttttat taaaactaag tccaatacta tatttacccc
    13681 tacagatata atcccattta ccataggtgt ttttgtgtct tgtaatgaat aaaaaacctt
    13741 tcccaaaata tctcttaatc caaatgcagt cataccaact gcataaaaaa ttaaggcagt
    13801 tgctgtcatt tgggttgccc tcgcatcaaa agcacctctt tcaaaaatga ttctaacaac
    13861 aggagttgca aaaaaaatag aagcaactga aataggaatc atagaaatta ttatcatatt
    13921 tatactagat ttaactgaac tagtaaactt cttttgatta ttttctgcac ttaacttaga
    13981 taacatagga tacataacag atactattga agctgtaaat gtccctgtaa caaaaccatt
    14041 taatctatct gaataagtaa gtgctggaat gcttccttta actaatgttg aggctaaagt
    14101 tgtatctacc aatgagttca cttgattaac agcaacacct ataaatactg gtgaaagtaa
    14161 tgctagtaat ttaataaggc tatcatcttt aaagtttaag tagtataagt atttgtagtt
    14221 cgttttttta acaaaaaaca tgtaaaagag cagttgaact accatagcaa ccacagcacc
    14281 aattggaagt atgtatggtc caaatacggt acttaacatt attgaaataa ttattactat
    14341 attatagggt atactcccaa atccaactac aataaagttc tctttaattt gcagaaatgc
    14401 tgacataaca ctcgtaatac ctatgaatat tattcctgtt atcaatattt tagtaaattt
    14461 tacggtaagt aaaaacctct ctccttcaaa tcctatagca aatatactta caagttgctt
    14521 tgagaaaata actcctaata tagctacaac tatgcatata ccaactatta tatttaaaac
    14581 attgtttaaa aactttagtg cttgcttttc accttgttta ctacttatat cttgatacat
    14641 tggaataaaa gtagtaacta tagcagtacc tattgctgca aatataatat ttggtatatt
    14701 catagctgtt aaataactct ctgtatataa gcctgtacca taaattgatg ataatactaa
    14761 ttctctaccc attccaagaa tcttagagat tatagtaact atcatcaaat aaaaagttgc
    14821 tttagctacc ttgctcattt tatcactcca cttctaacat acctcttttt aatcaagtaa
    14881 aatttggctc attagatata attctatcaa ctaatttgcc ttgcttagcc ctatcaaaat
    14941 ataattaaca cctatttctt aatatatcac aaactacatt attcttcata tttttttatt
    15001 tctccaataa gttttacatc tttcctatat aaattgtata gcttcaatta tattcaaaga
    15061 aatggagttg ctacccgata actttaaaag taaaaaacag attttattat ctttttaaac
    15121 tatattctaa catattttgt ccatattgag aaattttact aaactattat gtactttatt
    15181 aaataaataa aataacagca cacctataaa atacaagtgt gctgttcata aacagatttt
    15241 ataaaaaaat aattcctaag tagagattaa catgctttct catactctca tatataatgt
    15301 gtctaaaaat attaataaaa atgctttaat tgttttaact tacttggatg tcttaatttt
    15361 cttattgctt tagcttctat ctgtcttatt cgttctctgg taactccaaa tacctttcca
    15421 atctgttcta gtgttttagg gtcatcatca tcaattccaa atctcattct taaaacttgc
    15481 tgttcttgct caccaagacc tgtaagtagt tcttctatct tttcttttaa attatgctgt
    15541 tcaacttcgt gtattacaac atctttaaaa tcagcatctg atggtataaa ttcaactaaa
    15601 ctactatcct catcttcttt taatggagta tccaaagata caacattttt atctcttctt
    15661 aataattcca acactttgtc aacttctaag ccacatgcat ctgcaatctc atctatagtt
    15721 ggctctctta ataatacatt taaaagttct tgtttctttt tctttacaaa atttattctc
    15781 tgatgaagat gtataggtat cctaatagta ttttcgcaat cgtcgatata tcttgttata
    15841 ctttgtttta tccaccaagt tgcataagtg ctaaatttgt atcctttttt ataatcgtac
    15901 ttttctacag cttttataag tcctatattt cctgcttgaa ttaaatccaa catatcaata
    15961 ctatctcttt tatatctctt tgctatgctc actaccaacc tataatttga atttataaat
    16021 ttttcttttg ctactgaaga tgagttgatt atttccttag ccagctctac ctcttctcct
    16081 gctgacaaca ttttgtattc ctctatttcc tttaaataca ttttcatcgc atttgataca
    16141 tttgatgaac ccacacaaat ataattttca taattatttt cttttttagt atccataatc
    16201 aaaccccttc tattcataga atttgctaaa acaaaaataa ataatttaaa cctacataat
    16261 ctacaattac ataatatccc attctttaaa tgaattgttt tgtatattat gtatacaaat
    16321 ttataaaaac tatacctata atattattct acttctaacc gcaatatcct ccttatatat
    16381 ataatttatt ttattatatg ttgttatatt cttagttttt tgaatgatat atgtctaaaa
    16441 aaagactgtt tactaaacag tcttttttta tatatatgct caatatttaa ttttcatgat
    16501 gatgattaca tccacatcca caatttttat catggtgatg atgttcttct aaatcttttg
    16561 cctgttgttc catttcatga aactctttta ccattatatc ataagttgga agagctgcct
    16621 cagtatcgta ttcttcccct ctccaatcag cgtacatcat agcatcccca ttagttaaaa
    16681 ccaatctacc tgacatagtt atattatcca cttcttctaa aaattctatt tgtttttgta
    16741 aaacttctcc ttctaactct acatcttcat ctaaacattg cactatcatt acaggtgcat
    16801 aataatcttc ttcatcttta acatttacaa caatatctaa aactccttct gcaccttctt
    16861 caaattctgc caactcaaca tttgtatcaa tcatatcttg agggactaat aaatcttcta
    16921 ttaaatattt tattactcta tcttttacta attcttttga catttatttt tctccttccg
    16981 attctataac attatcataa tcatttctat attatagtat actacattaa ttgttttttc
    17041 aatctcttac aaaaattatt tttttattat cttccttata ttcccttttt tattacaaag
    17101 ttgtaatcgc attgatttaa aaaatagttt atatttagta taatagaaat gtagcacata
    17161 tttttattca atatattaat aaaaaaatag attgaaaatg taatgtatgt taaatctagg
    17221 aggaatatta tgaagtttaa aaaacaaatt agttctattg ttgtgtctac tatgcttgta
    17281 cttgggagta tgaacctttc ttatgcagat ggtacaaatg tagttacact tggagctaac
    17341 ttatcagagt ctcaaaagca gcaaatgctt aattactttg gagtaaaaaa agaccaagtc
    17401 ttagttttag atgtaacaaa tgcagaagaa agacagtatt tacaaggtgt tgcaacagag
    17461 ggtcaacttg gaaaagttac tatctcttgc tcatacgttg aacctactaa aaaaggaaat
    17521 ggaattaatg ttaaaacagc aaacttaact tgggtaacta gttcaatgat tgcttcaacc
    17581 cttacaacag ctggtcttga agatgcaaat gttattgctg ctgctccata tcctgtaagt
    17641 ggtactggag cattgacagg aatcatgaaa gcatttgagg atgcttcagg aaagaaactt
    17701 gatgaaacta agaaagaaat tgcttctgaa gaattggtag ttacaggtga cttaggagat
    17761 gatatagggc aagaaaaagc aacaggtgta atgaatgata taaaaacaga aattgtaaag
    17821 aatggtacaa aagataccaa tcaaatagca aatacaataa ataatgttgt aaataattac
    17881 aatattacta ttacaccaga acaaaaagaa caaattaaag gtgtaatgaa aaaaatagct
    17941 gaccaaaatt atgattacaa tagcatgaaa aatacactag ataatgtatc agataatgta
    18001 aatgaacaat taaagaaatt aggtgaaagt gttaagggtt ctggtatact agatactatt
    18061 ggtggatggt ttagtggaat aggagactgg ttctctggtt tattctcagg cggagataaa
    18121 aaagatttgg gaatcttaaa taacactaat gatgagtctc ttggctctga tgctgtaata
    18181 aattctacag aaggtgttca aataaatcca caaaataata ctaattctaa caatgatagt
    18241 gatacaaata gctcaaaaaa tgatacttca agtaataatg aaaataacga tagttctcaa
    18301 acaaatgaaa acaacacaac aaataattca gaaaatcaat caaataataa tgactcagtt
    18361 ggtttctttg aaaaaataaa aaattggctt tcaggcttat tttagaaatt aaacaaataa
    18421 aaagaattgc ctaaaatgga aattttctat aaaaggcaat tctctttatt tacaataaat
    18481 ttttattact cactcatttt tttagatttt tctatacatg cacatattgc ttttatcaca
    18541 gaagctctaa aaccttcttc ttcaagtact ttaactgctt ctatagtagt tccaccaggt
    18601 gaacatacca tatcttttaa ttcaccagga tgttttcctg tctcaagtac catttttgca
    18661 gaacccataa cagcctgtga tgcaaactta tatgcttgtg gtcttggcat tcctgcaatt
    18721 actgctgcat ctgctatagc ttctataaat aaaaatacat atgctggtgc agaaccactt
    18781 gcaccaatta cagcttctat aagatactct ggtactacct cagtatttcc aaaagaatta
    18841 aatacttctg taactgcttc taagtcttca ttgtttatat ttttatttat agaaagagaa
    18901 gacatggctt catttacaag agctggagta tttggcatag ttctaactat ttttttatct
    18961 tctcctataa ttgactctat atcctttata gattttcctg ctgctattgt aactataatt
    19021 ttattgtcat ctataaagtc ttttatctct tccaaaatat catcatatat atctggttta
    19081 acagctacaa ttactatatc agaggttcta gttacttcct tcgagtcttt tgtagtattt
    19141 attccaaaat ttgcatctat tctatcgaga gttccttgac tatatgctga tgctataacc
    19201 atattaggtt caactaattt tgaatttact attcccccta tcatagcgct tgccatattt
    19261 cctgcaccta tgaatccaat ttttttcact ttagtacctc cttattgact aatagtaatc
    19321 gttaaaattt aagcttcttt aactgcatca gacttttcaa atggatttgg tatacttact
    19381 accaaaattt tcactacctt attagtatag ttgacccaat tatgattgtt cttttcacta
    19441 ttatagtgta ttgtgtctcc tggatacatt tctatttgtt catctcccaa aaacactgta
    19501 agagtaccct ctaatacata cacaaattcc tcaccttcat gtacgtaaca acaaatttcc
    19561 tcactactct tacttggaag aatttcaatt aatcttggta gcattgtttt ttcctttaaa
    19621 ctagatgata aatgataatg tataaatgtt gagttttcta catcaaatac ttctttttca
    19681 taacttctta atacagctct tttatgctct tttggcttca taaaaaagta agttaattct
    19741 acatctagta cacttgctat ttttcctaag gaatctgttg ccacacttgt taatcctctt
    19801 tcaagttgtg ataaaaaacc aattgacaag tttgtttttt cactcatatc cttcagcgtc
    19861 atttgctttt gagttctgag ttgctttatt tttgctccta tgtccttatt caaaaccatc
    19921 acccctaaat aattttaatt ttttcataat ataaatacaa aaatataaat ataggtactg
    19981 aacctatctt tatttcattt aaaaaaaatt tttataagta ttactataat ttatcaatct
    20041 ttttttgtct acatttttac gattatttac gatttatttt tcacatatgt atatttaatc
    20101 ttgccaacta aagatacatt ttttataagc atttactctt tatataataa aacttaagtt
    20161 gttcatgtag tcttcttctg gtttaattat taaaatttca aattatatta ttatttctac
    20221 acattattac atcctttagt acaattataa cataatttta caatttaatg atatctattc
    20281 aatgtctttt tttactctac aacatatttt attttagagt ttaatactat atatattttt
    20341 tatatacagc cttgtcattt tttagattgt caagagttat tgaagtattt tatgtagatt
    20401 tatattttat tttctaattt ctcatttaac aagaattgct tatgataaaa aataatatca
    20461 tctcaataag attcttgtaa atatatctat attctaaata taatttcttt ttttgtcaaa
    20521 atttcttcat tctttaataa ttaatttatt acttataaaa taaactaatt atctatacaa
    20581 attttataat ttttaataaa atacacaaaa aaattaattt ttatagctca tttcgccaaa
    20641 atatatcttt ctttatatca ttaaaataat tataaattta ttaaatatgt ttgtaattct
    20701 ataaagttat ttttatcaaa ctaaaa
//
LOCUS       DAGQWR010000075        20659 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.075, whole genome shotgun sequence.
ACCESSION   DAGQWR010000075 DAGQWR010000000
VERSION     DAGQWR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20659
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.075"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            358..669
                     /gene="rpsJ"
                     /locus_tag="MIW95_002298"
     CDS             358..669
                     /gene="rpsJ"
                     /locus_tag="MIW95_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBY3151620.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            761..1390
                     /gene="rplC"
                     /locus_tag="MIW95_002299"
     CDS             761..1390
                     /gene="rplC"
                     /locus_tag="MIW95_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBY3151621.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1420..2043
                     /gene="rplD"
                     /locus_tag="MIW95_002300"
     CDS             1420..2043
                     /gene="rplD"
                     /locus_tag="MIW95_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBY3151622.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2043..2333
                     /gene="rplW"
                     /locus_tag="MIW95_002301"
     CDS             2043..2333
                     /gene="rplW"
                     /locus_tag="MIW95_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBY3151623.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTASFKKAVVKLTADSKEIEFFQGM"
     gene            2363..3193
                     /gene="rplB"
                     /locus_tag="MIW95_002302"
     CDS             2363..3193
                     /gene="rplB"
                     /locus_tag="MIW95_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBY3151624.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3228..3509
                     /gene="rpsS"
                     /locus_tag="MIW95_002303"
     CDS             3228..3509
                     /gene="rpsS"
                     /locus_tag="MIW95_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBY3151625.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3541..3876
                     /gene="rplV"
                     /locus_tag="MIW95_002304"
     CDS             3541..3876
                     /gene="rplV"
                     /locus_tag="MIW95_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBY3151626.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3899..4714
                     /gene="rpsC"
                     /locus_tag="MIW95_002305"
     CDS             3899..4714
                     /gene="rpsC"
                     /locus_tag="MIW95_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBY3151627.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4750..5181
                     /gene="rplP"
                     /locus_tag="MIW95_002306"
     CDS             4750..5181
                     /gene="rplP"
                     /locus_tag="MIW95_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBY3151628.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5183..5386
                     /gene="rpmC"
                     /locus_tag="MIW95_002307"
     CDS             5183..5386
                     /gene="rpmC"
                     /locus_tag="MIW95_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBY3151629.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5410..5664
                     /gene="rpsQ"
                     /locus_tag="MIW95_002308"
     CDS             5410..5664
                     /gene="rpsQ"
                     /locus_tag="MIW95_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBY3151630.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5690..6058
                     /gene="rplN"
                     /locus_tag="MIW95_002309"
     CDS             5690..6058
                     /gene="rplN"
                     /locus_tag="MIW95_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBY3151631.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6082..6387
                     /gene="rplX"
                     /locus_tag="MIW95_002310"
     CDS             6082..6387
                     /gene="rplX"
                     /locus_tag="MIW95_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBY3151632.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6417..6959
                     /gene="rplE"
                     /locus_tag="MIW95_002311"
     CDS             6417..6959
                     /gene="rplE"
                     /locus_tag="MIW95_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBY3151633.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6977..7162
                     /locus_tag="MIW95_002312"
     CDS             6977..7162
                     /locus_tag="MIW95_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBY3151634.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7195..7593
                     /gene="rpsH"
                     /locus_tag="MIW95_002313"
     CDS             7195..7593
                     /gene="rpsH"
                     /locus_tag="MIW95_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBY3151635.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7624..8166
                     /gene="rplF"
                     /locus_tag="MIW95_002314"
     CDS             7624..8166
                     /gene="rplF"
                     /locus_tag="MIW95_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBY3151636.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8202..8570
                     /gene="rplR"
                     /locus_tag="MIW95_002315"
     CDS             8202..8570
                     /gene="rplR"
                     /locus_tag="MIW95_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBY3151637.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8591..9100
                     /gene="rpsE"
                     /locus_tag="MIW95_002316"
     CDS             8591..9100
                     /gene="rpsE"
                     /locus_tag="MIW95_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBY3151638.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9115..9300
                     /gene="rpmD"
                     /locus_tag="MIW95_002317"
     CDS             9115..9300
                     /gene="rpmD"
                     /locus_tag="MIW95_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBY3151639.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9333..9776
                     /gene="rplO"
                     /locus_tag="MIW95_002318"
     CDS             9333..9776
                     /gene="rplO"
                     /locus_tag="MIW95_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBY3151640.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9820..11088
                     /gene="secY"
                     /locus_tag="MIW95_002319"
     CDS             9820..11088
                     /gene="secY"
                     /locus_tag="MIW95_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBY3151641.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11105..11755
                     /locus_tag="MIW95_002320"
     CDS             11105..11755
                     /locus_tag="MIW95_002320"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBY3151642.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11756..12502
                     /gene="map"
                     /locus_tag="MIW95_002321"
     CDS             11756..12502
                     /gene="map"
                     /locus_tag="MIW95_002321"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBY3151643.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12524..12793
                     /locus_tag="MIW95_002322"
     CDS             12524..12793
                     /locus_tag="MIW95_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBY3151644.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12831..13049
                     /gene="infA"
                     /locus_tag="MIW95_002323"
     CDS             12831..13049
                     /gene="infA"
                     /locus_tag="MIW95_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBY3151645.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13085..13198
                     /gene="rpmJ"
                     /locus_tag="MIW95_002324"
     CDS             13085..13198
                     /gene="rpmJ"
                     /locus_tag="MIW95_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBY3151646.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13351..13722
                     /gene="rpsM"
                     /locus_tag="MIW95_002325"
     CDS             13351..13722
                     /gene="rpsM"
                     /locus_tag="MIW95_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBY3151647.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13758..14156
                     /gene="rpsK"
                     /locus_tag="MIW95_002326"
     CDS             13758..14156
                     /gene="rpsK"
                     /locus_tag="MIW95_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBY3151648.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14188..14811
                     /gene="rpsD"
                     /locus_tag="MIW95_002327"
     CDS             14188..14811
                     /gene="rpsD"
                     /locus_tag="MIW95_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBY3151649.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14889..15836
                     /locus_tag="MIW95_002328"
     CDS             14889..15836
                     /locus_tag="MIW95_002328"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBY3151650.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15857..16198
                     /gene="rplQ"
                     /locus_tag="MIW95_002329"
     CDS             15857..16198
                     /gene="rplQ"
                     /locus_tag="MIW95_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBY3151651.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16318..17151
                     /locus_tag="MIW95_002330"
     CDS             16318..17151
                     /locus_tag="MIW95_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBY3151652.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17139..18005
                     /locus_tag="MIW95_002331"
     CDS             17139..18005
                     /locus_tag="MIW95_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBY3151653.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDISFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            17999..18802
                     /locus_tag="MIW95_002332"
     CDS             17999..18802
                     /locus_tag="MIW95_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBY3151654.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18834..19565
                     /gene="truA"
                     /locus_tag="MIW95_002333"
     CDS             18834..19565
                     /gene="truA"
                     /locus_tag="MIW95_002333"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBY3151655.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19685..20116
                     /gene="rplM"
                     /locus_tag="MIW95_002334"
     CDS             19685..20116
                     /gene="rplM"
                     /locus_tag="MIW95_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBY3151656.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20145..20537
                     /gene="rpsI"
                     /locus_tag="MIW95_002335"
     CDS             20145..20537
                     /gene="rpsI"
                     /locus_tag="MIW95_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBY3151657.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8252 a         2437 c         4318 g         5652 t
ORIGIN      
        1 atggatttaa tataaaaaaa agaggggggt aactcctctc ttttttgttt tttgttgaca
       61 agtagcgcta aagcatatat actaaactag tgccttgaga cagattaaca atcaagttta
      121 aggttatgat gttgtaatag aaaataaaat tgaaaaaaac agaaaaaaaa cttgaaaaaa
      181 tgtaacagat gatataaaat ataatagtgt gcttgagaga ttaagaaaat aataattaaa
      241 tcctagtgaa atacgaactg atatcgattt aaaagttaca acacacccgg agcagtgtat
      301 cggtattggt tgtacgcaac ttgaaaatta aacacgtgat aaaggaggga aaacaaaatg
      361 gctaagaatg aaaaaataag aataagatta aaatcatatg atcacaaatt attagatttt
      421 tcagcaggta aaatagttga aactgctaaa aaggctggat cacaagtttc aggacctgtg
      481 cctctaccaa cagaaaaaca agttgtaact atattaagag ctgtacataa gtacaaagac
      541 tctagagaac aatttgaaat aagaactcat aaaagactaa tcgacatagc taatccaaca
      601 cctaagacag ttgactcatt aatgagatta gacttaccag caggtgtaga tatagaaata
      661 aaattataag aacccctaag atattatcct atagatgatt atcttagaat gattactcta
      721 aaagatgtaa tccgctgtaa gaattatagg aggtgctaat atgaaaggaa tattaggcaa
      781 aaaagttggt atgactcaaa tattcactga taaaggtgta gttatacctg taacagctgt
      841 tgaagcaggg cctatggtcg ttactcaaat aaaaacagtt gataaagatg gatacaatgc
      901 tatacaaata ggttttgaag atgctaaaga aaaagcttta aacaaaccta aaaaaggaca
      961 tttagctgct gctaatgttt taaagaaaca tttaaaagaa tttagagtag attctgtaga
     1021 aggatacaca gtaggacaag aaataaaagc tgatgtattt gaagcaggtg caaaaataga
     1081 tgttactgga ataagtaaag gtaagggatt ccaaggtcca ataaaaagac atggacaaag
     1141 tagaggtcct gaaactcatg gttctagata ccacagaaga ccaggttcaa tgggagcttg
     1201 ttcttaccca ggtagagtat ttaaaaacaa aaaattagct ggacatatgg gaagcgtaaa
     1261 ggtaacagtt caaaacttag aggttgttaa agtagatgct gacaagaacc ttatattagt
     1321 taaaggagct ataccaggag ctaaaggttc agtagtaact ataaaagaag ctataaaggt
     1381 ttctaaataa tgactaacct agagaaagga ggaataacaa tgccaaaatt aaatgtacta
     1441 aatgttagtg gacaaaacgt tggagaaata gaattatcag attctatatt tggcgtagaa
     1501 gttaatggac atgttttata cgaagttgta aaaaatcaat tagcaaataa gagacaagga
     1561 actcaatctg ctaaaactag agcagaagtt agaggtggcg gaagaaaacc ttggaaacaa
     1621 aaaggaacag gtagagcaag acaaggttct acaagatctg tacaatgggt aggtggagga
     1681 gttgcttttg cacctaaacc aagaagctac aaatatacat tacctaagaa ggtaagaaga
     1741 ttagctatga agagtgcttt atcttcaaaa gttcaaaata gtgaagttat agtattagat
     1801 gcattaaaca tggatgctcc taagactaaa gaatttgctc aaatattaaa caatataaat
     1861 gctgctaaaa aagctttagt agttatagct gacaaaaacg ataacgtaat aaaatcagct
     1921 agaaatatag aaggtgttca aactgcatta gttaatacta tgaatgtata tgatatatta
     1981 aaatacgatt catttataat aactacagat gctgttaaaa aagtggagga ggtgtacgca
     2041 taatgactaa tccacatgat gtaataataa gaccagttgt aactgagcac agtatggctg
     2101 aaatgggtga aaaaaaatac acttttgttg tggctaaaga tgcgaacaaa actgaaataa
     2161 aaaaagcagt agaaaaagta ttcggagtta gtgttgataa agtaaatact ttaaactacg
     2221 atggaaaagt taaaagaatg ggtagaactc aaggaagaac agcaagcttt aagaaagcag
     2281 tagttaaatt aactgctgat agtaaagaaa tagaattctt ccaaggaatg tagtattata
     2341 acaaacgaag gagggaaaac agatggctat aaaaaagttt agaccaactt ctcctgcctt
     2401 aagacaaatg acagttttag tttctgatga aataacatgt aatcaaccag aaaaatctct
     2461 tttagttaac ttaaagaaaa atgctggtag aaacgtacat ggtagaataa ctgttcgtca
     2521 tagaggtgga ggacaaaaaa gaaaatatag aataatagat tttaaaagag ataaagatgg
     2581 tatacctgct aaggttgcta ctatagaata tgatccaaac agaactgcaa atatcgcatt
     2641 attaaactat gctgatggag aaaaaagata tatattagct ccagttggaa ttaacgttgg
     2701 agatactata ttatcaggat taggtgctga tataaaacca ggaaactgtt tagcattaaa
     2761 agatatgcca gttggtacta tcattcataa tatagaatta aagccaggaa aaggagctca
     2821 attagttaga tctgctggag tttctgcaca attaatggct aaagaaggaa aaaatgcttt
     2881 attaagatta ccatcaggtg agatgagatt agtaagcata aactgtaaag ctactatagg
     2941 tcaagttgga aatatagagc atggtaacgt agttatcggt aaagcaggta gaaaaagaca
     3001 tatgggaata agacctactg taagaggttc tgtaatgaac cctaatgacc atccacacgg
     3061 tggaggggaa ggtagatctc caataggtag accatctcct gttactccat ggggtaaacc
     3121 agctcttgga tacaaaacta gaaagaaaaa taaagcttca aataaactaa tagtatcaag
     3181 aagaactaag tagtaaattt atttttgctc ggaaaggagg aatataaatg tcaagatcaa
     3241 ctaaaaaagg gccttttgtc catgcaagac ttttaaagaa gatagaagct atgaatgcta
     3301 gtggaaataa agaagttata aaaacttggt caagatcttc aactgtattt ccacaaatgg
     3361 tagaaaacac tatagctgtt catgatggaa gaaaacatgt tccagtatat ataacagaag
     3421 atatggttgg acataaatta ggtgagttcg ttcctactag aactttcaaa ggacataagg
     3481 atgacgaaaa atctaataag agaaaataat taaattatga ctaagaaagg aggaataaca
     3541 atggaagcaa aagctactgc taaatacgta cgtgtatcac caagaaaagc aggccaaata
     3601 tgtgaccttg ttagaggaaa gaatgttgat gaagcattag caatattaaa gtttactcca
     3661 agaggagcag cttcaataat agctaaggtt gtaaaatcag ctaaagctaa cgcagaaaac
     3721 aatcacgaaa tggatactga aaaattatat atagcatcaa tagttgctaa tcaaggacca
     3781 acaatgaaga gattcatgcc tagagctatg ggtcgtgcaa ctacaataag aaaaagaact
     3841 tctcatatag aggttgttgt taaagaaaaa aaataattaa tagataggag ggaaagtaat
     3901 gggtcaaaag gttaatccac acggcttaag ggtcggtgtt ataaaagatt gggactcaag
     3961 atggttcgct actgataaaa aagagttcgg aaacttatta ttagaagacc ataatatacg
     4021 taaattctta aagaaaagat tatactcagc tggagttgct aagatagaaa tagaaagatc
     4081 agctaacaaa ataaaaatgg acttacacgt tgctaagcct ggtgtagtta taggaagagc
     4141 tggtgcagga atcgaagcat taaaagctga attagaaaaa atgacaaaga aaactataat
     4201 agttaatata gtagaagtaa gaagtacaga taaaaatgct caattagtag ctgaaaatat
     4261 agctttagct atagagagaa gggttgcttt cagaagagca atgaaacaag ctatacaaag
     4321 agcaatgaaa tctggtgcta aagggataaa agtttctgct tcaggaagat taggtggagc
     4381 tgaaatggct agaactgaag gttatagtga aggaaatgtg ccactacaaa ctttaagagc
     4441 agatatagat tatggtttcg ctgaagctga tacaacttat ggaaaaatcg gtataaaagt
     4501 ttggatatgt aatggtgaag ttttaccaac tagagacggt gtaaatccaa gagaagaaag
     4561 cagaaagagc gatagaagag acaacaagag agataataga agaaacgata gaagaggaaa
     4621 tgacagaaga ggaaatgata acagaggaaa ctacagagga caaagacctc aaggtggatc
     4681 aagacctcaa agaactgaaa acaaaggaaa ttaataatag ttaggttcaa ggaaggagga
     4741 aaaatactca tgttaatgcc aaaaagagta aagcgtcgta gagttcatag aggaagcatg
     4801 gctgggcaag ctcataaggg taataaagtt acttatggtg agtttggatt agtagcatta
     4861 gaagcttctt ggataacttc taatcaaata gaagctgcca gaatcgcgat gactagatat
     4921 ataaaaagag ggggaaaggt ttggataaaa atattccctc ataaaccagt aacaagaaaa
     4981 ccagcagaga ctcgtatggg tgcagggaaa ggttctccag aatattgggt agctgtagtt
     5041 aagccaggaa gagttatgtt tgaattagca ggtgtttctg aagataaagc aagagaagct
     5101 atgagacttg ctgcacataa attaccaatc aaatgtaaat ttgttaaaaa agaagattta
     5161 gaagtaaagg gtggtgaata ggatgaaagc taaagaatta agagatttaa caagcgaaga
     5221 gctaatgaac aaattaaacg acttcaaaag tgaattattt agcttaagat tccaattagc
     5281 tactggtcaa ttagaaaata cagctagaat aaagtttgtt aagaaggata tagcaaaagt
     5341 taaaactgtt cttgctgaaa gaaagttata cgaaactaga gcttaatttg gaaaggaggc
     5401 tttttaaaca tggaaagagg aagaagaaaa gttagaatag gccgtgttgt tagtgataag
     5461 atggataaaa caatagtagt agctgttgaa gaattcgtac gtcatccact atataataaa
     5521 cgtgttaaga gaactaagaa attcaaggct catgatgaaa agaatatatg taacatcgga
     5581 gatagagtta aaataatgga aactagacca ttatctaaag ataagagatt cagactagtt
     5641 gaagttgttg agaaagttaa gtagtttttg aagaaggagg gattacgata tgatacaaca
     5701 agaatcacgt ctaagagttg ctgataactc aggagctaaa gaacttttat gtatacgtgt
     5761 tctaggcgga agtaaaagaa gatatggtaa cataggcgac gttatagttg ctactgttaa
     5821 aagtgcaaca cctggtggag ttgtaaaaaa aggtaaggtt gttaaagctg ttatagtaag
     5881 aagcaaacaa ggcgtaagac gtaatgacgg aagctatata tcttttgatg aaaatgctgc
     5941 tgttataata aaggacgata aaactccagt aggaactcgt atattcgggc ctgttgctag
     6001 agagttaaga gacaatgaat tcatgaaaat agtttctctt gctccagaag tactataatt
     6061 aaggaggtgc aataggacat gatgcgtgtt aaaaaaggtg acactgttgt agttatagca
     6121 ggtaaagata aaggtaaaaa aggttcagtt ttaaaggtat atcctaaaac tagcaaagtg
     6181 ttagttgaag gtgtaaatgt aataactaaa catcaaaaac caagtgctat gaaccaacaa
     6241 ggtggaataa taaataaaga agccccaata cacatatcta atgtaatgcc atttgatcct
     6301 gaaacaggaa aaggcgttag agtaagatat gaagtaaaag atgggaacaa agtaagagta
     6361 tcagcaaaga gcggaaaaga attataataa atattgcttg aaaggaggga ccttaaatgg
     6421 cttctagatt acaagaaaaa tacatgaaag aagttgctcc tgctttaatg gagaaatttg
     6481 gatacaaaaa cgtaatggag atacctaagt taaataaaat agttattaac atgggtatag
     6541 gtgacgctag agaaaatcca aaaggattag aaaaaggcgt tgaagaatta gaaatgatat
     6601 caggacaaaa gcctgttata actaaagcta gaaaatctgt tgctaacttc aaattaagag
     6661 aaggaatgcc aataggaaca aaagttacat taagagctga caaaatgttc tactttatgg
     6721 acaaattagt ttcagtttct ttaccaagag ttagagattt tagaggagtt aaccctaatg
     6781 cttttgatgg tagaggaaat tatgctttag gagttaaaga acaattaata ttccctgaaa
     6841 tagaatatga taaaatagat aaagtaagag gaatggatat aatatttgtt actacagcaa
     6901 aaactgacga agaagctcgt gaattattaa aattattagg aatgccattt tctaagtaag
     6961 caaaggaggg attcccgtgg ctagaaaagc gatggttgtt aagcaacaaa gaaagcaaaa
     7021 gtacgctact agagaataca ctagatgtac tatatgcgga agaccgcatt cagttttgaa
     7081 aaaatttggt atatgccgta tatgctttag agaattagct tataaaggtc aaatacctgg
     7141 tgttagaaaa gcaagctggt agaagcttga ttagaatgga aggagggcaa caaaatgaca
     7201 atgacagatc caattgcaga tatgcttact cgtataagaa atgctaatgt tgttaagcat
     7261 gaaactgtag atgttccagc ttctaatatg aagaaagaat tagctagaat cttattagaa
     7321 gaaggtttca taagaggtta tgatgttata gaagatggaa aacaaggtat cataagaata
     7381 caattaaagt acggacaaga aggcgaaaga gttataacag gtcttaagaa aatatctaag
     7441 cctggaatga gagtttacgc tgctaaccat gagataccaa aagtattaaa cggattagga
     7501 atatcagtta tatctacttc aaaaggaata ttaactgaca agcaagctag aaaagaaaat
     7561 gttggtggag aagtaatctg ctacgtttgg taattaatat gaacaatgta aggaggtgcg
     7621 accatgtcaa gaataggtgt taagccaata ataataccag caggtgttga agttactata
     7681 gctgaaggaa atttagttac agtaaaaggt ccaaaaggaa ctttaactaa acaattaagt
     7741 gctgagttaa acataaaaaa agaagaaaat acaataatgg ttgaaagacc aactgataat
     7801 aaaaaacata gatctttaca cggactaact agaactttat tagataatat ggttgtaggt
     7861 gtaaatactg gtttcgaaaa gaaattagaa ttaaaaggtg ttggatatag agctcaaaaa
     7921 caaggaaaga aattagttat gaacttaggt ttctctcatc cagttgagat ggaagatcca
     7981 gaaggaataa ctgttgaagc tccaaaccaa acagagttaa tagtaaaagg tatagacaag
     8041 caattagtag ggaattatgc tgctaagata agagcttgga gaaagccaga gccatacaaa
     8101 ggtaaaggta taaaatacgt tgatgaagta atcagacgta aagaaggaaa aactggtaaa
     8161 aaataatacc aaaacttaaa cgctagaaag gagtgagcta ggtgttaaaa aaggctgata
     8221 aaaatgctaa tagacttcaa agacataaga gagttcgtag aaaaataagc ggtacttctc
     8281 aaagaccaag attatgtgta tttagaagtg ctaacaatat atatgctcaa ataatagatg
     8341 acacaaaaag agtaactcta gttgctgcat cttctttaga agcagaagtt aagagtgctg
     8401 tcaatcatac aggaaacaag gaagcagcta agaaagttgg agaattagtt gctaaaaaag
     8461 ctgttgaaaa aggaatcact gaagttgtat ttgacagagg tggatactta tatcatggaa
     8521 gaattcaaga attagctgaa ggtgctagag aagctggtct taagttctaa tacgaaggag
     8581 ggaaaaatac atgctacgtc gtaagccgat agatgcagga caacttgact tacaagaaaa
     8641 agttgttgaa gtaagacgtg ttactaaggt tgtaaaaggt ggtagaaatt tcagatttgc
     8701 agcattagta gttgttggag atgaaaacgg acacgttggt ataggtgctg ggaaagcaat
     8761 ggaagtacca gacgctataa aaaaagcagt tgaagatgct aagaaaaatc taatagtagt
     8821 gccaatagtt gggactacaa ttcctcacga agtacgcgga cactttggtg ctggaaatat
     8881 attaataatg cctgctgtag aaggtacagg agtaatagct gggggacctg ctagagccgt
     8941 acttgagcta gctggactaa aagacgttag agctaaatct ttagggtcta acaatccaag
     9001 aaatatggta aatgctacaa tagaaggact taattctcta aaaacagttg aagatatagc
     9061 taaacttaga ggtaaaaaag ttgaagaact tctagggtaa ggaggtagtg taaaatggct
     9121 aaattacaaa taaagttagt tagaagtgtt ataggaacta ctcctaacca aaaaaagaat
     9181 gttgaagcgt taggattaag aaaaagagaa caagttgttg taaaagaaga caatgctcaa
     9241 acaagaggta tgataaataa agtaagtcac ttattagaag taactgaaat agctgaataa
     9301 tttttataaa gtagtgcaag gaggtgcaat ccatgaagtt acatgagtta aaacctgctg
     9361 aaggtgcagt aagagctaag agaagattag gtagaggtac tgcaactgga caaggtaaaa
     9421 ctgcaggacg tggacaaaaa ggtcaatggt ctcgttctgg tggtggagta agagtaggat
     9481 ttgaaggtgg acaaatgcca ctagctagaa gacttcctaa gagaggtttc aataacatat
     9541 ttaagaaagt ttacacagaa gttaatgttg aagttttaaa cagatttgaa aatggaacag
     9601 aaataactgc agaattatta aaatctacta aaactataag caaaataggt aaagacggaa
     9661 ttaaaatctt aggtgaaggt aatttagaga aagctttaac tgttaaagct gctaaattta
     9721 cagcttcagc tcaagaaaaa atagaaaaag ctggtggaaa agcagagtta gtataataac
     9781 ctgctaatct cctagccagt acaggcgggg tgaattagcg tgctgtcaaa attaaaacaa
     9841 gcttggaaga ttaaagcagt aagaaaaaaa gtaatgtata cacttatgat gatcgtgata
     9901 ttcagaatag gtactactat accagttcca ggcatagaca ctagtattat acaaaagatg
     9961 gtaggcggca atagtctact ttctctgtat aatatgttta ctggtggagc atttagtaac
    10021 ttttcactat ttgctttagg tataagtcca tatatcactg catcaatcat aatacaactt
    10081 ctaacagtag gttttgaaag tttagcagaa cttcaaaaat ctggtgaaga aggaaagaaa
    10141 aaaatcaata agtacactaa atatacagca ctagctctag cagttgtaca agctttggga
    10201 attacattag gaattgtaag aagtgcttta atatcaaata gtgtgttttt tatcacaact
    10261 gttgtaataa cattagtttc agcaagtatg ttagtaatgt ggattgggga caaaattact
    10321 gaaaaaggaa tcggtaatgg tagttcagta ataatatttg caggtataat atcaagaata
    10381 cctacagatg ttattaaaat atcgcaacaa gttaaatctg gcgaagttgc accttgggtt
    10441 atagtgattc tagctgtagt tatattactt acagtaacag gagttacatt tatacaagaa
    10501 gcaactagaa agatacctgt acaatatgct aaaagagttg ttggaagaaa aatgtatgga
    10561 ggacaaagtt ctcatatacc aatgaaagtt aaccagtcag gagttatgcc tataattttt
    10621 gctagttcac ttttagcttt ccctcaaaca atagctatgt ttatgggtcc aaatgctcag
    10681 gcttttgttc aaaaatactt aagtatggca acagaacaag gattttggac atatagatca
    10741 attgagattc tacttataat tttcttctca tatttttata caactgtatc atttaataca
    10801 gaagacatat ctaaaaatat gaaaaataat ggtggattta taccaggtat aagaccaggt
    10861 gagcctacta tggattattt aaatagaatt ttaactagat taactttagc tggagctact
    10921 ttcttagcaa taattgctat ggtgccagct cttacaacac attatatgaa agtaaatatg
    10981 agtctagctg ggacttcatt gctaatcgtt gttggagttg ctttggagct taagagacag
    11041 ttagagtcaa acttagttat gagaagctat caaggtttct taaaataaaa tggagatgga
    11101 taatatgaga ataatattac ttggacctcc aggtgctggt aaaggtactc aggcggcagg
    11161 aatagtagaa aaatacaata tacctcatat atcaacagga gatatattca gaaagaatat
    11221 aaaagaggga acagaacttg gaaaaaaagc taaagaatac atggaccaag gtttattagt
    11281 accagatgag ttaactgtag gtttagttac tgatagaata tctcaagaag attgtaaaaa
    11341 tggatttatg ttagatggat ttccaagaaa tgtagcacaa ggagaacatt tagatatctt
    11401 cttaaaaaat gctggtatat cactagataa agttgtcaat attgaagttg ataagagtat
    11461 attagtgtct agagcagttg gtagaagaat atgtaagtct tgtggagcta cttaccatgt
    11521 tgagtttaat cctcctaaag tagaaggtgt atgtgatgta tgccaaggag aattatatca
    11581 aagagctgat gataatgaag aaactgtatc taagagaata caagtttatc tagatgaaac
    11641 taagccttta gtagattatt atagcaaaca aggtataata gcagatataa aaggtgatca
    11701 agcaatagat aaagtatttg aagatattgt cgcagcctta ggaagtggaa aataaatgat
    11761 tatcttgaaa tcaaaaaaag aaatagaact tttaagagaa gcaggtaaaa tagttgctga
    11821 cactcatgaa gttttaagaa aagctatttc tccgggaata tctactttag aattagataa
    11881 aatagctgaa gagaatataa gaaaatacaa tgcagagcct tcttttaaag gatatggagg
    11941 ttttccagga agtatatgtg cttctatcaa tagagaggta gtacatggta ttccaggaga
    12001 aactatatta caagaaggtg acattgtaag tttggatata ggagcctatt ataaaggata
    12061 tcatggtgat tctgcgaaaa ctcacggagt aggcatgata tctgaagaag ataggaaatt
    12121 gatagaagta actagagaaa gcttctatga aggaataaaa tttgctaaat taggatatag
    12181 actttcagat atctcacacg cagtacaaac acacgttgag aaacatggtt tctcggtagt
    12241 tagagattta gtgggtcatg gagttggagc aaatcttcat gaagacccac aagtacctaa
    12301 ttatggactc ccaggaaaag gaccaagact tagagaagga atggttattg ctatagagcc
    12361 aatggttaat gcaggtcgtt atcatgtaaa gactctatca gatgggtgga cgactgttac
    12421 aatagatgga aaaaaatcag ctcattatga gcatacgata gcaattactg aacatgaacc
    12481 tttgatattg acaaagttgt aatttaaaaa gtaggtgaaa aaagtgctat cagataattt
    12541 aagtataggt caagttgtta aagtttcatt aggacgagat aaaggaaatt tattctttgt
    12601 agtcaaaata attaataatg aatatgtatt aatagctgat ggaaaaaaga gaaaacttga
    12661 caaacctaag ttaaagaaag tgaagcactt aaaaaaatat aattttatta atgatgaagt
    12721 tagaaaaaga gtagtatctg gacaagagat tacagattct tttttaagag ctgaacttac
    12781 taagttaaat tagataattt aacttattga aatggagggc tggttagtta atggccaaaa
    12841 aagatgttat agaattagaa ggtacagttt cagaagcttt acctaatgct atgttcaaag
    12901 ttaaactaga aaatggacat gagatattat gccacatttc tggaaagcta agaatgaact
    12961 tcataagaat tcttgaaggt gacaaggtga atgttgaact ttctccatat gaccttacaa
    13021 gaggaagaat tacttggcgt aagaagtaga ctatattaat atttagtcta aggagggatt
    13081 aataatgaag gttagaccat cagtaaaacc aatatgtgaa aaatgtaaag taataaaaag
    13141 aaaaggaaaa gtaatggtta tctgtgaaaa tccaaagcat aagcaaaagc aaggataaga
    13201 aattattact agcattttgt taaattttat agtataatac tatattgtga tgttaacaaa
    13261 agaacgttaa aatcgttgaa caatctggcg taggattggc atatgatgtg aagttttaaa
    13321 cactaaaaac tatgtgtagg aggtgcgatc atggcaagaa tagctggggt agatttacct
    13381 agagaaaaaa gagcggagat aggcttaact tatatatatg gtataggtaa agcaactgct
    13441 aacgaaatat tagctaaggc tgagataaat ccagatacta gaataaaaga tttatcagaa
    13501 gatcaagtta atgagttaag aaaagttata gatgacgatt tcttagttga aggtgactta
    13561 agaagagaaa ttgctttaaa cataaagaga ttaagagata taaaatgtta tagaggaata
    13621 agacacgcta aaggtcttcc attaagagga caaagaacta agactaatgc tagaactaga
    13681 aaaggtccta gaaaaacagt atctcgtaaa aagaaaaaat aaataaataa taactagatg
    13741 aaggagggaa aaggaaaatg gctaaaccaa aaaagaaagt tacacgtatt agaagaagag
    13801 aacgtaaaaa tatagaacgt ggtcatgctc atatacaatc aactttcaat aatacaataa
    13861 taactttaac tgacgttcac ggaaatgcta tatcttgggc aagttctgga caattaggat
    13921 tcaaaggatc aagaaaatca actccatttg catctcaaat ggctgctgaa acagctgcaa
    13981 aagctgcgat ggaacacgga ctaaaaagtg ttgaggtatt cgtaaagggg ccaggttcag
    14041 gaagagaagc tgcaataaga gctttacaag caactggact agaagtaact atgataaaag
    14101 acgttactcc aatcccacat aacggatgca gaccaccaaa gagaagaaga gtgtaattaa
    14161 taagagtaca ataggaggtg tagataaatg gcaagatata caggtgcatc atgtagacaa
    14221 tgccgtagag agggaatgaa attattcctt aaaggtgata gatgttatac agacaaatgt
    14281 gctatagtaa aaagaaacta tgctccaggt caacatggcc aaggaagaaa gaaagtttct
    14341 aactatggat tacaattaag agaaaaacaa aaagttaaaa gaatatatgg agttttagag
    14401 actcaattta gaaatttata tgaacgtgct gaaaatatgc ctggtaaagc aggtgagaac
    14461 ttattaagtt tattagagag aagattagat aatgtagttt acagaatggg actagcatct
    14521 tctagaaaag aagctagaca attagtgact catggtcatt tcactttaaa tggaaataaa
    14581 gtagacatac cttcattaat agttaaggtt ggagatgtta tagaagttaa agagaaatca
    14641 agatcttctg caaaattcaa aaatttagta gaagttaact caagaatagc tcctaagtgg
    14701 ttagaagcta atgtagaagg aatgacagca aaagtagttg gtgttccaac aagagaagat
    14761 atagatcttg agatagcaga gcacttaatc atagaacttt actctaaata ataagcacac
    14821 ttttaaaaat tattttaaaa ttttgtttac cctcagtgga ttactgaatt ttaaggaggg
    14881 ttttgtccat gatagaaata gaaaagccaa aagtagatat agttgagctt agcgaagact
    14941 atagatatgg taagtttgtt atagagcctc tagaaagagg atatggaata actataggta
    15001 acgcattaag aagaatatta ttatcatcat taccaggtgt agcagtgaat gctataaaaa
    15061 tagatggagt tcttcatgaa ttttcaacaa tacctggtgt taaagaagat gttactgaga
    15121 taatattaac tttaaaagag ctttcagcaa ctatagatgg tgaaggaagt agaacactta
    15181 aaatagaagc tcaaggacca tgctctatca caggtgcaga tataatctgt cctccagatg
    15241 ttgaaatatt aagtaaagat ttagcaatag ctacattaga tgataatgct aaacttaata
    15301 tggagatatt tgtagataaa ggtagaggtt atgtttctgc tgaagaaaat aaaacagaga
    15361 atgttccaat aggtgtttta cctgtggatt caatatatac tcctgttgag aaggttagtt
    15421 atcatgtgga aaacacaaga gttggtcaaa aaacagatta tgataaatta gtactagaag
    15481 tttggactaa tggtagtata aatcctcaag agggaatatc attagctgca aaggtattag
    15541 ttgagcattt aaacttattt atagacttaa ctgaacatgt aagtagtgtt gaaatcatgg
    15601 tagaaaaaga agaagatcaa aaagaaaaag ttcttgaaat gactatagaa gaattagatt
    15661 tatcagttag atcatataac tgtttaaaga gagcgggaat taatacagtt gaagaattag
    15721 ctaataaatc tgaggatgat atgatgaagg ttagaaacct aggtaagaag tcattagaag
    15781 aggtaataca aaaactagaa gaacttggat tgggattaaa accaagtgaa gaatagcgag
    15841 caaaggaggg ataagaatgg ctaagtaccg taaattagga cgtgaaacag ctcacagaaa
    15901 ccttatgtta agaaacttag taacttgctt actaagaagt ggaagaatag aaactacagt
    15961 gactagagcg aaagaaactc gtagaatggc tgaaaagatg ataactcttg ctaagagagg
    16021 agatcttcat gctagaagac aagttttagc ttatgttatg gatgaaacag tagttaataa
    16081 cttattcaca gatttagctc caaaatatgc tgagagaaat ggtggataca ctagaataat
    16141 aaaaataggg ccaagaaaag gcgatgctgc tgaaatggct tttatagaat tagtatagta
    16201 aggaaaggat taggcttgat ggcttaatct tttttttatt tttttgacta aggtatgata
    16261 taataatgtg taaaatgatt tttataagtt attgcttact aaaaggagta tgagttaatg
    16321 gataatatag taaaggtaaa taatatttcg tttgagtata ttacagatga agcaaaactt
    16381 aaagcaatag ataatttaag cctagatgtt aaaaaaggag aatttgttgc gataatagga
    16441 cataatggtt ctggtaagtc tactttatct aaaaacttaa atgctattct tatgcctact
    16501 gaaggaaata ttcttattga tgatatggat actaaagaag aagaaagatt atgggatata
    16561 agacaaactg caggcatggt atttcaaaat ccagataatc aaatagttgc tactatagta
    16621 gaagaagacg ttgcatttgg acctgagaac ttaggtatag aaccaaaaga aataagacga
    16681 attgtagagg agtctttaaa aagtgtaggt atgtatgatt taagagatag acagcctcat
    16741 ttgttatcag gaggtcaaaa gcaaagagtt gcaatagcag gaattattgc tatgaggcct
    16801 aaatgtataa tatttgatga agcaacagca atgttagatc catctggaag aaaagaagtt
    16861 atgaaaacaa taaaaagact taataaggaa gaaaatataa ctgttataca tataacgcac
    16921 tttatggaag aagcagtaga agctgataga gttgtagtta tggaaaaagg aaagaaaatc
    16981 ttagagggaa ctcctagaga agtattcagt aaaataaaga tgttaaagga aatcggttta
    17041 gatgtacctt gtatgacaga gttatctagt ttattaatag aagaaggtat aaatattagt
    17101 agtgatatat taactgtgga tgagatggtg atggaattat gtcaattata gtaaagaatt
    17161 taacacatat atataatgaa gggatgcctt ttgcaagtaa ggcacttgat gatatttctt
    17221 ttgagattaa agatagagat tttgttggtc ttataggtca tacaggctca ggaaaatcta
    17281 cattaataca acatttaaat ggattactaa agccttcctc aggagaaata tttataaatg
    17341 attttaatat aacagataaa aacttgaatt taactgagat tagaaaaaga gtaggggttg
    17401 tatttcaata tccagaatac caattgtttg aagaaacgat tgataaagat atagcttttg
    17461 gaccctctaa tttaggctta gaggagtctg agatacataa cagggtaaaa gcatcaatgg
    17521 aagctgtagg acttgattat gagggattta aagacaaatc tccttttgaa ttatctgggg
    17581 gacaaaagcg tagagtagca attgctggag ttatagccat gaacccagaa gttctcatat
    17641 tggatgaacc aactgctggt cttgaccctg gtggaagaga tgaaattttt aacttaataa
    17701 aagacttaca tgaaaaaaag aatatgacta taatactatc atcacatagt atggatgata
    17761 tggcaaagtt agcaaaaact ttaattgtta tgaatcatgg tagcgtagaa tttatgggaa
    17821 ctcctagaga agtgtttaaa tcaaatgcta gtaaattaaa agatatagga ttagatattc
    17881 cacaagtttt agagcttgca ttgaaattga gagaaaaagg ctttgacatc agtgaggata
    17941 tattgacttt agaagaagca aaacaggaaa tattaaaagt tgtgagagga cgaggattat
    18001 gttaaaagat ataactatag ggcaatacta cccaacaagt tctgctattc ataaattaga
    18061 tccaaggata aagcttgtag cgacaattgt atttatggta tctatatttg tagtaaataa
    18121 attttggcct tatatagtag ttttattatg tttattagct atgataaaat tggctaatat
    18181 accagtcaaa tatattgtta agggtgttaa acctcttaaa tggattattt tgtttacatt
    18241 tcttataaat atttttttct taccaggtga tgaaatatgg tcatttggat ttttagctat
    18301 tacaaaacag ggtctaagac aagcaatttt tatggcaata agattaatat ttttagtagt
    18361 tggtacatca ttgcttactt taacaacttc accaatagaa ttaactgatg gaatagagag
    18421 attgttgaat ccatttaaga aaattggttt gccagttcat gaacttgcaa tgatgatgac
    18481 aattgcttta cgttttattc ctacattgtt agatgagaca gacaaaataa tgaaggctca
    18541 gatgtctaga ggtgcagatt ttgaaagtaa gaatcttata aatagagcaa agaatttagt
    18601 accacttttg gtacctttat ttgtaagtgc atttagaaga gctgatgaat tggctatggc
    18661 catggaggcc agatgttaca gaggtggaca taataggact aagatgagag agtctgttat
    18721 atcaagaagg gattatatgg catgtgtatt tcaggttgtt tatctgggag caattatagc
    18781 tactagattt atagcaattt aattaataag ttatagatat aatgaggtaa agtatgagaa
    18841 atataaaaat taaaatccaa tacaatggta agaattattg tggatggcaa aagcaacctg
    18901 attctttagg gatacaaggt acaattgaaa gggctatata tgatataaca aaagaagaaa
    18961 ccagtcttat agggtctggt agaactgatt caggagttca tgctattggt cagattgcaa
    19021 attttaaaat taattcaggt ataagtatag aaagtatacc aatggcacta aatgccaagt
    19081 tgccaaaaga catatcagtt atagaagcat gtgaagtaaa tgatgatttt cactccagat
    19141 acagtgcaaa aggtaaaaca tataaatatt tagtatataa tagcaagttt agaaatccaa
    19201 tacttagtga gatatcatat caagtaaaat atgaacttga ttttgataaa atgtgttcag
    19261 aagcaaaaag tttattagga acacatgatt ttaaaggttt tatgagttct ggttcatctg
    19321 taaaagatac agttagaacg atatatgata ttgatataag taaaaaagat gacttgatta
    19381 cttttgagat tagtggtaat ggttttttat ataacatggt gaggattatt gttggaacac
    19441 ttgttgatat gggtcgtggc agaattaacg aaccattttt agatataata caatcaaaaa
    19501 ctaggtcaag atgtggacat acagctccag cacaaggatt atttctcaaa aaagttcatt
    19561 attaacttga cacaccaggc tacatgtatt aaaatatatg agaatgtgtt aataagtcca
    19621 cttgccccgg acttgacata aacggtaaaa aaattttatg aaaagtagtt aaggagggac
    19681 agttatgaaa agttatatag ctaagccagc tgatgtacaa agaaaatggt acttagttga
    19741 tgctgaagga aaaacattag gtcgtttagc aacagaaatt gcgacagtat tgagaggtaa
    19801 acataaacca acatttactc ctcacgttga tggtggagat tttgttgttg tagtaaatgc
    19861 agaaaaaata gttttaagtg gtaagaaatt agatcaaaaa tattacagat accatactgg
    19921 ttatgtaggt ggattaaaag aaatatctta cagagatatg atggataaga aaccagaaga
    19981 agttatatca catgctgtaa gcggtatgtt acctaaaaat aaattaagaa gcagaatgat
    20041 gactagatta agagtatttg caggtgctga gcacactcat gcagctcaaa atccagaagt
    20101 tttaaacttt aaataagatt aataggtgaa aggaggagtt tataatggct aacgttcaat
    20161 attatggaac tggaagaaga aaaagttctg ttgctagagt aagactagtt gcaggtgaag
    20221 gaaatatatt agtaaatgga agagcattag aaaattattt taattatgag acattaataa
    20281 gagatgttaa acaaccatta gttttaactg gtaatgaaaa taaatatgat gttatagtaa
    20341 aagttgaagg tggaggattc actggacaag ctggagctat aagacatgga atatctagag
    20401 ctttattaaa agctgattta gatttaagac ctgctttaaa gaaagaaggt ttcttaacta
    20461 gagatgcaag aatgaaggaa agaaagaaat acggattaaa agcagcaaga agagctccac
    20521 aattctcaaa aagataatta cttatttttt tggaaaagca aaatccttta tttactgtta
    20581 ttcagaaagt attggtattt taaaaatgtt gggttaagtt aattattgat atataataaa
    20641 ataataagat atcttatta
//
LOCUS       DAGQWR010000076        20615 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.076, whole genome shotgun sequence.
ACCESSION   DAGQWR010000076 DAGQWR010000000
VERSION     DAGQWR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.076"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            57..1871
                     /locus_tag="MIW95_002336"
     CDS             57..1871
                     /locus_tag="MIW95_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY3151658.1"
                     /translation="MTLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPEYLNVWELQ
                     GITINSKNNPKTLNGQDLEKMGLNLKDYNVTQECIIEDITSRKDVNKYLRKTSAPITE
                     LTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPILLVEKNNV
                     PNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRYETSLLIAK
                     EIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTYKWLKSEDL
                     QNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFYTEDEIEAV
                     LVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVYKIGGGLTS
                     KVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKDYNLNTSLA
                     TTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNKQGHGVEVF
                     YKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVLRSNDMPAV
                     LVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            2099..4012
                     /locus_tag="MIW95_002337"
     CDS             2099..4012
                     /locus_tag="MIW95_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151659.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTDNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEINTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEENVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLRMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            4005..6224
                     /locus_tag="MIW95_002338"
     CDS             4005..6224
                     /locus_tag="MIW95_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBY3151660.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEIALEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLISENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            6333..7163
                     /locus_tag="MIW95_002339"
     CDS             6333..7163
                     /locus_tag="MIW95_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBY3151661.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            7153..10620
                     /gene="addB"
                     /locus_tag="MIW95_002340"
     CDS             7153..10620
                     /gene="addB"
                     /locus_tag="MIW95_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBY3151662.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIEN
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDDYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSTICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            10620..14447
                     /gene="addA"
                     /locus_tag="MIW95_002341"
     CDS             10620..14447
                     /gene="addA"
                     /locus_tag="MIW95_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBY3151663.1"
                     /translation="MSSPKWTKEQLEVIGSRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIANISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDIDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNETESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDSPDSDY
                     YSEIEKKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            14493..15704
                     /locus_tag="MIW95_002342"
     CDS             14493..15704
                     /locus_tag="MIW95_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBY3151664.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVIATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            15691..19218
                     /locus_tag="MIW95_002343"
     CDS             15691..19218
                     /locus_tag="MIW95_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY3151665.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNIQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFVVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKDLKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRS
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVINITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            19349..20602
                     /locus_tag="MIW95_002344"
     CDS             19349..20602
                     /locus_tag="MIW95_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBY3151666.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
BASE COUNT         8957 a         1943 c         3660 g         6055 t
ORIGIN      
        1 taggtagcaa atcaaaagtt agtgtcaaat ttaaagaaaa accagatgca gatagcatta
       61 cattaaagta taaatgctat gacatgccat tggatacaac tctaaattac aatcaatcaa
      121 ctggggcata tgaaggaact atcaattata accaagaccc agaatatcta aatgtttggg
      181 aactacaagg gataacaata aacagcaaaa ataatcctaa aactttaaac ggacaagacc
      241 tagaaaagat gggattaaat ttaaaagact ataatgtaac acaggaatgt ataattgaag
      301 acataacttc tagaaaagat gtaaataaat atttgagaaa aacttctgca cctattacag
      361 aacttacagg aagtgataga tatgaaacag cagttaaaat aagtaaagag ggctggaaaa
      421 atggttcaga taaagtagtt ataataaatg gagatgtaag tatagatggc attatatcaa
      481 ctccgctggc aaccacatat aatgctccaa tacttttggt tgaaaaaaac aatgtgccta
      541 atagtgtaaa atcagaatta aagcgcctaa accctaaaga tataattata attggagatg
      601 agaatgctat ttctaaaact actgctaatc aaattaaatc gactgtaaat gctagtcaaa
      661 cacgtttaaa tggttctaat agatatgaga catctttatt gatagcaaag gaaatagata
      721 aaaatcatga tgtggaaaaa gtatacataa caaatgctaa tggcggagaa gtggatgcac
      781 ttactatagc agcaaaagca ggtcaagaca agcaaccaat tatattaact gataaagata
      841 gtattacaga caatacatat aaatggttaa agagtgagga tttacaaaat gcttatttta
      901 taggtggtcc tcaaatgata tcaacaaatg ttataaataa ggtaaatgga ataactaagg
      961 atagtgttac taataataga gtatacggag cagatagaca cgaaacaaat gcaaatgtaa
     1021 taaaaaaatt ctatacagag gatgagatag aggctgtttt agtagctaaa tcagatgtac
     1081 ttgttgatgc tttagcagca ggtccattgg ctgcgaactt aaaatctcca atacttataa
     1141 caccaaagac gtatgtatct gcataccata aagataattt agaagctaaa tcagctaata
     1201 aagtatacaa aataggtgga ggattgactt ctaaggtaat gagctctata gcatcatcat
     1261 tatctaaaca caatacgact ccaacagaac caggaaatag tgggggcaag acagttatga
     1321 ttgacccagg gcatggtggt tcagcacctg gaaattcatc tggaggaatg attgaaaaag
     1381 attacaattt aaatacttca cttgcaacaa ctgaatattt acgttcaaag ggattcaatg
     1441 taataatgac aagagacaca gataagactt tatctcttgg aaatagaact gctctatcta
     1501 attcattgaa accagattta tttacaagta tacattataa tggctcaact aataaacaag
     1561 gtcatggtgt agaagtattt tataagctta aagataaaaa tggggggact actaaaactg
     1621 tagctaccaa tatattaaat agaattttag agaaatttaa acttacaaat agaggtataa
     1681 aaacaagagt acttcctagt gattctacaa aagattattt atacgtttta agaagtaatg
     1741 atatgccagc tgtacttgta gaatgtgcat ttttggataa tgaaaatgat atgagtttaa
     1801 taaactcatc tgcaaaagta aaagaaatgg gtacacaaat aggtaaagga atagaagatt
     1861 cattaaaata aatggaaata aaaatattta aaaacttaat gtaaatttat actaaagtca
     1921 aaaaatgaaa tataagtgta tcaatagtaa gactgtatgt acatttgcac atgtgaatgc
     1981 aagtatatat acagtctttt tttgtagtta aatgcaataa tttgattgtg ttttgtaaaa
     2041 tacacaatat gatataatta gataatatac acttaattta attgaggaag gtgattatat
     2101 ggaatattta tttgaaaaaa gtagtttaaa agaatatgga aatgaagctt tagataaata
     2161 tagagaatta ttagataaaa ctgataataa ttcaaggtgt attcttttat tagtaccaaa
     2221 caataacaca aagatacgat atgaaagggc tctaagatta aattatagtg aagaactgaa
     2281 aataacaaca tatataagtt ttgtgaagaa agaattagtt aaatactggc cactaataat
     2341 agaaaaatgt gatgaaataa atacaaaggt tgtatctcca acatttatat caaatagttt
     2401 aagtgaatat ttaatagtgc aaaaagttaa ggaaaaaagg atacaagaag gatattttga
     2461 agatattaca ggaaccaata aatccatagc aaatagtata atgactaaca taaataaatc
     2521 tgcatttaat ttaattgatt ttgattttat tggagaaaaa atatattcat caaagaaaaa
     2581 taaagatagt atatatagat tttcatacac tcagatggat gaaattatat catattatat
     2641 aaattcgtta ctatcaaatt caattttaga taatgcatta agcatatatt tatacaatca
     2701 atacctactt aatgatgatt tttatttaaa aaacttattt acagaagtga gatatataat
     2761 tgtggatagt cttgaaagct caagtaatgc agaagttgat tttataacag aagcattgga
     2821 taatacatta caaagttata tatattttga ttatagtagg gattattctg tatttaataa
     2881 tgtggatata gactatataa atgagaaaat aatatcaaaa attaagccta aagaagaaaa
     2941 tattaaaaag aaggagatta gaatagaaga tttatactta ttacctgcaa atatagaatt
     3001 aaatcaatca agtcaattat acaatgaaat gttagattta ataagtgaaa aagttatatc
     3061 tcttatagaa gaaaatgtat ctccaagaga tatagcaatt atatctccta taaacaatag
     3121 tatattggag catcaaatta gagattcttt gatagaaaaa aatatagatg tttttagtac
     3181 taagaaggat aaaaaagctg tagattatcc atatgggaat gcattggtag tggctacatg
     3241 tatattttat ggatatttag attttataaa agatgatgaa tacttaagct ttttagaaac
     3301 tcttttagaa ataaatagga taaaagcatt taagattttt aatgaaacaa gacatttaag
     3361 tattgataat atagatgaac atagtcagta tcgtgatatt ttacagtata ttgaagaaaa
     3421 aaagaagagt gatataaaaa tacatgaatt tttaactcaa ttttacatag ataaaatgtt
     3481 aaatttaaaa gaaggtaaaa agaatgttgg gttatgtaaa aagataatag ctgaaagtga
     3541 gtcttttagt gaaagtttaa gtctacttag gatgaaaaaa gaaaagatat ttatagaagc
     3601 tttaaaaagt acaataaacg attattactc tgttgtcgat atagaagagt tgaaatccaa
     3661 agacaaaata gtaataacta ctccttattc atatatatca tcaaatatag atagaagtat
     3721 acaaatatgg gtagatatag gaagtaatgc ttggaatatg aaaatagaaa aagatataag
     3781 taatttaatt gtacttagaa aatcttttga agaaaaaaag atttatacaa atgaaatgga
     3841 agaatattat aaaaaatatt atctatacaa tacagtatat aatttgttat taaatgcaaa
     3901 gaaaatctat gcatttaaaa gtgaatatgc gattaatggt tatatccaag agagtatatt
     3961 atatagtata cttttaaaaa tctcacataa aggagataaa aattatgatt aatataaatt
     4021 atagagaaga ccagatacca ataataaatt acgataatgg tactatggca gtaccagcag
     4081 taccaggtgc tggaaaaaca tttataataa caaatttagt agctaaacta ttacttgaac
     4141 agaaacataa gggtggtaaa atattaattc ttacatatat gaatagtgct gtaaataatt
     4201 ttaaaggtag aataagaaag attcttgaag agaataaaat agatgatact aattcgtatg
     4261 aagtaatgac tatacacagt ttggctgtaa aaataataaa ggaaaagcca gaagttgtaa
     4321 tgttaagtga ggattttaac atagctgacg atttgcaaaa aagtattata ttaaatgaat
     4381 gtataagtag atttagattt gaaggtggag aaagagcttt tagatggttt ttaaaggagc
     4441 aaaaagatga acgctggaaa gaaatagctc ttgaagcctg ggaaagaggt ttttttgaat
     4501 ttgcaggaaa tgcaataagt gaacttaagt ataagggaat ttctccagat aaacttgaag
     4561 aaatattgtc aagaggacac aaaggaatgc ttaaaattat tttaccaata tataaattgt
     4621 atgatagaaa attaaagcaa aatggattgc tagattatga tgatatatta attttagcag
     4681 aaaaaacttt aagcttagac gaagggctta gaaagaagtt tcaaactaga tacaaatata
     4741 tatttgaaga tgagtgtcag gactcaaatg aaattcaagg taatataata aaaattatat
     4801 ctagtgaaaa taaaaaccta gttagagttg gagatataaa tcagagtata acaggaacat
     4861 tttcatcatc agaccctaaa ttttttaaag aatttataaa tagtgcagat ttctgttata
     4921 ggatggatat gtcaaatcga agttcaaaag atatacttga tttagcaaac actcttgtca
     4981 agtatgttac aaaggaattt agacaagaag aatgtagaca tgctcttgaa gatatgcaaa
     5041 taaaaactgt gccaaatgga atgggatata aagaaaatcc taatccagaa cattataata
     5101 taaatgtaaa gtggtatgag agttggaaaa ttgaaataga gcaaactgct agatacgtta
     5161 agggtataaa aaataaatat ccagataaaa gtataggtat attggttcca tttaatgaac
     5221 aagtgacaca agttgctaaa gaacttagag agaataatct aacctttgaa gaattaggac
     5281 caaattcttt gaataaaagg aaggttataa acaacatagc atatattatt gattttatgt
     5341 tacattgtga tgatgtagaa aagttaatag ttgtacttaa taaagttttt atagatactg
     5401 ataatgaaga agaaataaag tattttttag aagaattaaa aaactattct accgaagaaa
     5461 ttatttataa ttttgaattt agtaagttag ataatgattt aatagaattt aaagaagaaa
     5521 gtgacatata tttaagtttt aaaagaggaa tagatattat aaaacagata ttagaatatc
     5581 caataataag gttagacctc ttagttttgt ttattgggaa aaaacttaat ttagaaaaag
     5641 aagataaagc tgtactggat tatatatctt tttatataaa gtatctaata tcagaaaata
     5701 taaatatgga tttagaagat gtatataatt tattgtttga tgtaaagaat agggtgttta
     5761 atcatataat agatgtggta tatgagataa atggatatga accagagccc ggtagtataa
     5821 cactatgtaa ctatcataag tcaaagggga tggaatggga ttgtgtgttt ttactaggtc
     5881 ttgttgaata taatttccct gacaatatta atcaaaagtt tcagagtgat aaatggtatc
     5941 taaaagaaaa atataaaaac cctatggcaa ttattaaatc agaagttgaa gctatattaa
     6001 aaggaagtat ttcaacagat tatgctcata aaacgaagat tgattctata aatgaaaaga
     6061 ttagactttt atatgtaggg attactagag caaaggaaat gttagtactt tcaggaagtg
     6121 cttatagaga tgaaagtgat atagggaata agagaaaaga gcaaaaacca tgtatatatt
     6181 taagcagact taatcaacat attattgaaa aaaggtcaaa ttaatatata ataagttatt
     6241 aaaagattgc ttgaaatata tctaaaaggg gataaaacaa tatgtacagt aagtaactac
     6301 taaaaaataa agttaagaga gaagtgatat gagtgaacga taaattaaaa tatttttctt
     6361 atagtcaaaa ttcaatcaac acatataaat catgtccatt gaaatttaaa tataaatata
     6421 ttgatagaat aaattggaaa aatgatgatg ttggtagtag agagtattat gaaactttaa
     6481 aaacaggaag agattttcat ttgatttgtg aaagatattt tagcaatata cctcttggaa
     6541 tctattttaa tgaggatgat aaaaatagta aaaagttttt aaagtggatg gaaaatataa
     6601 aaaaagttgt tcctattgaa aaaggaaaaa cgtatttgcc agaatatgaa gtaaggatga
     6661 ccttaaatgg ggatataata caggcaaagt atgatttagt tgtaattgga aataacaata
     6721 tagaaatatg ggattggaaa accgagtcta aaaagattga ctataaacac gtagaaaata
     6781 gaatacaaac tattgtttat atgtttttag caaaagaagt aatacctaaa gttttaaaaa
     6841 tggacataaa tgttaaagat ataaatatga gatattatca acctgaattt gatgacttac
     6901 caattaccat tttatatgat gaagaaaaac atgaagcaaa tagaaataaa attcaaaatt
     6961 atataagtat gataaaaaac actaattatg aagaacatac atacgaagac aatctataca
     7021 atgatataga aaaggtatat aataaagaag aacgtatgtg ctatagaaat aaaaagcatt
     7081 gcaagtattg tgaatttaat aagttatgta atggcaaaga aatagattat agtattttgg
     7141 aggcggaaat ttatgggact taggtttgta ttaggtagaa gtggaagtgg aaaatccacc
     7201 tatatactag atgagattaa aaaagaagct caaaaaaatg aaacgacatc aattatactt
     7261 ttagttccag aacagtacac atttgaggct gaaaatagag taagtaagct attcttagga
     7321 aaagaaaaag ataagtatct aagagttagg gtacttagct ttaagacact aagtaatata
     7381 gttttttctc aagtaggagg attgacagat gtaaatataa attctagtgg aaaagcaatg
     7441 atggtatata gagctattga agatgtaagt gaagaattaa atgtgttttc aaaatcaaaa
     7501 tcgcagtctg gttttgtaag ctcaattaca gatatgatat cagaaatgaa acaatacaat
     7561 ataagccctg aaatgttaga aaatatatct ggagaattgg ataatgaaac tttaagttta
     7621 aaattaaaag atataagtaa aatatataat tcttttgaag ggaaattgca tgaaaattat
     7681 gtagatgctc aagatatgtt aacttcattg gcaagtaaaa ttgaattaag cagttattta
     7741 gatggagctt gtgtttatat agatgaattt acaggtttta caccaaatca gtacaatgtc
     7801 ataaagagta tattaaataa gtctaaaagt gttaatatct ctttgactgt agatgatatc
     7861 aactatattg ggtacagtaa atctgatatg ttttctagaa cgaaatttac ttattcaaaa
     7921 ttgactcaat tgtgcaacga agaaggcata aaaatattgc cacaagtaaa tttaaacaca
     7981 ggtgttataa aaagatttga aaaagttaag gaattacagc acctagaaag attttataat
     8041 gcttatccat ataaaatata ttcaaatcca actgaaaata taaagataaa agagtttaac
     8101 aatttatatt cagaggtgga agagatagcc agagaaattg tacatttagt tagagataag
     8161 aatgtaagat atagagatat aaccattgct acaagagatt taaatagata tgatttttta
     8221 gtacactcta tatttaatga atataatata ccaaacttta tagataagaa gagagaggca
     8281 aaaagtaatc caatagttat tttaattatt tcagctttag aaatgaagaa tagacgatat
     8341 gggtatgaaa ctatgtttag gtatttaaaa agtggactta taggaattga taatgatgat
     8401 ataaatttac tagaaaatta tgtgttggct aatgggatta aaggtaaaaa atggtttgat
     8461 gaaaaatggg attatagaat aacacaaagt ctttcaggtc aagaaagtga atttgaactg
     8521 gaattaaaag aaaaaataaa tgaaataaaa aatagagtat tagagcctat agtaatttta
     8581 caagaaaaat taagaggaaa aaatagagta aaagaaatct gtagatatat atatgaattt
     8641 ttattagata taaatatgcc agaaactata gaaaatttaa tagtaaattt taaggataaa
     8701 ggggaacttg atgttgcaaa tcaatactca caagtttggg acatagtagt ggacatatta
     8761 gaccaaatgg tggaacttat gggtgatgaa ataatttctt tagaaaaatt tataaaatta
     8821 ataaccttag gctttgatga atatgaatta ggactggttc ctcctagtat agaccaggta
     8881 cttgtaagta gtgtggatag aatgaaaaat ccagacacga aatatttata cctagttgga
     8941 actactgatg gtgtgtttcc actgattaca aaggatagtg gaatattgag tgataatgat
     9001 agggaaagtc ttggaaacaa aggaatcgaa gttgatattg atagtaagac taggagtttt
     9061 gaagaacagt ttcttgtata taaagctctt acttcgacat ctaagaattt aaccataaca
     9121 tatccaatat cagaccatga aggtaaaacg cttagaccat caataattat atcaagactt
     9181 aagaagatat ttccaaatat agaaaataaa agttatttag tggaagaaaa taaaagcact
     9241 gataaagata tacttaaaaa aataacggtt aaatccccta catttaatga acttataaac
     9301 gtaataaaaa attatgacag tgatgattac aatacagaag agattaatag tatttggtta
     9361 gatatatata ggtactattt aaaagatgaa atctatagtt ctataacaaa aaaagttata
     9421 aaaggtctta gttatactaa tcaagttcat aagattgaag agaaaaaaat aaggtcattg
     9481 tataaaagta attctcttag catatctagg cttgaaaaat atgctgaatg tccatttgct
     9541 tattttatac aatatggtct aaaagcaaaa aagcgtaaag agtattcatt tacaccacca
     9601 gatttaggaa cttttattca taatatttta gataggtttt caaaagaact tttacaagat
     9661 aatttaactt ggagagatat tgatgaaaaa tatatagaat taaagatagg tataatagtt
     9721 gatgaaataa ttttaaagat accaggatat atattaaata gttctgagag gtataaatac
     9781 cttgcatata gattaaagaa tatgttgaca actgctataa ctataattag tcagcaaata
     9841 aaacaaggtt catttgaacc aatagattat gaagttaaat ttggcgacaa tggaaaatat
     9901 ccacctataa aaatggtgtt agaaaatgga caagaagtaa gtttaattgg acaaatagat
     9961 agagttgatg aatttgaaga aggagaaaat aaatatataa gaataataga ttataagtca
    10021 ggcaataagt ccataagtct aacagaaata tattatggtc tacaattaca attacttgtg
    10081 tatttagatg caatccttga aagtgcaaag gatgaagata taaatataaa tccagctgca
    10141 atactatatt gtagaataaa taatccaata gcaaaattta atgaagataa agatgatgaa
    10201 gaaatacaag aagcaatatt aaaagaactt agaatgaagg ggcttgtagt aaaagactct
    10261 cacatagtta aagagatgga taaatcatta atagatggag aaaggaaaaa ctctttagta
    10321 ataccagtag gtcttactaa ggatggaaat gtaggtaaaa gtacttcagc aataagctat
    10381 gaggatttca aactgcttag aaaatatgta aggcatgcta taaaggattt atgtgaagaa
    10441 atgttaagtg gagaaatacg tatagctcca tataaacata aagatggaac atcatgtgac
    10501 ttttgtgatt actctactat atgtcagttt gattcaacta taaaagataa caagtataaa
    10561 aatttaaata ataagagtaa tgaagagata ataaaaatga tgaaaggaga tgttaattag
    10621 tgagttctcc taaatggaca aaagagcaat tagaagttat aggaagtaga gaatgcaatt
    10681 tattggttgc tgctgctgct ggttcaggaa aaacagccgt tctagttgag cgtataattc
    10741 aaatgataac aagtagagaa aatccaattg atatagataa gttattagtt gttacattta
    10801 ctaatgctgc tgcgtcagaa atgagagaga ggatagggga tgctatagga aaagccttag
    10861 atgaaaaccc agaaaataaa catttacaaa accaacttgt tttattaaat aaatctagta
    10921 ttacaacaat acactcattc tgtttggatg ttataaaatc aaactttcat agaataaact
    10981 tagaccctaa tttcagaata ggagaccaaa ctgaatgtgc tattttgaaa caagaagcaa
    11041 tagaggaagt atttgaagat ttatatgaag aaagagatga ggggttttta aatttagttg
    11101 aaagttatgc agaaagagga ggagataaag aagtacaaga tattatcttg ggaatatatt
    11161 catttgcaat ggcttctcct gaacctaaaa aatggttgat tgactcagca gaacgattta
    11221 atattgatga aaattttgat ttttcacagt ctatttgggc tagagctata ttagacactg
    11281 taaaaataga aattaatggt ctttgtctaa atatggaaag ggcattaaaa gaagtggaat
    11341 caatagaaga attagaaact tttgcagaaa agttatccgt tgaatataaa aaaattgcta
    11401 acatttcaca agcatgtaat aaatcttggg atgaggcata taaaaagatg gcaagtatgt
    11461 cctttgaaaa ttatgtaaaa ggagtaaaaa gaatttcaaa agatgctcct tcgtatataa
    11521 aagaatcaaa agaaaaggca aaaactataa gagataagac taagaagtct ttagaaagta
    11581 tagtaagtgc tacattcaat aaagataatg attctattag agaagaaata aaatatcttt
    11641 ataacatagt taaaccaata tctagtgttg tactgagatt tgaagaggaa tatagcaata
    11701 agaaaagaga aaaaggaata atagatttta acgatataga gcattttgca ttaaatatac
    11761 ttacagatat agatgaaaaa ggtaatattg taccttctga tatagccgtt ggatatagaa
    11821 acaagtttta tgaaatattt atagatgaat atcaggatag taatctagtg caggaagtac
    11881 tgcttaaagc tgttgcaaat acagaaactc caaatagatt tatggttggt gatgtaaaac
    11941 agagtatata tagatttaga caagcaaagc cagaattatt tttgcaaaaa tataataact
    12001 ataatgataa aaagggaagt tcccatagaa aaataatgct ttacaaaaat tttagaagta
    12061 gagaagaagt tgtagatgct gtaaattata tatttgaaaa tataatgaat gagaatatag
    12121 gagaaattga atatacagaa aaagaaagac taaatttagg tgcgaatttt aatgtggata
    12181 cagacgagaa atctattatt ggtggagcaa cggagataca cctaatacaa aaagataata
    12241 aacttgatga tgatattata aatgataaag acgatagaat taataacaaa gaaaatgaaa
    12301 tagaggaaga agaaaaatta gataatatac aacttgaggc tagaatggtt ggaaacatta
    12361 tcaaagattt aatgaaagtc aatgaagatg gaaaaattca aaaggtctat gataagggaa
    12421 tagatggata tagacctgtg gaatttagag atatagttat tcttttaaga gctacatctg
    12481 catgggctcc agtgtttgca gatgaattga tgaatatgga tataccaaca tatgctgatg
    12541 ttggagtggg gtattttgat acgattgaaa taaaaacaat attgtcatta cttcaaataa
    12601 tagataatcc aatgcaagat atacctctta tatctgtatt aaaatcacct atatttggat
    12661 ttacaccaga ggatttgatt gatattagag ttcagagtaa agataaaatt ttttatgagg
    12721 ttttaaaaag tacagcagag tatgatggat ttacagattc tcaaaatgaa actgagtctg
    12781 agtttatacc aagtgaagag tgcataaaca aaagtaaaga ttttttaatt aagctaaagg
    12841 aatttaaaga aaagtcaatg tacatgagta cagatgaatt tatatggtac ttgtacacaa
    12901 ggactggata ctatgcttat gttggagcat tgccaggtgg aagtcaaaga caagctaatt
    12961 taaaagtatt gtttgagaga gcaaaacaat ttgaagaaac cagtcttaaa ggaatattca
    13021 actttgtaaa ttttatagag aaattaaaaa aatctagttc tgatatggga agtgcaaaaa
    13081 cacttggtga gaatgcaaat gttgttagaa taatgagtat tcataaaagt aaaggtctag
    13141 aatttccagt agtcatatgc tctgcaatgg gtaaaaattt caatactcaa gattttaaaa
    13201 agagtatttt atatcatcat aatttagggt atggacctca atttgtagac tatgagagaa
    13261 ggatttcttt tccaagtata gccaaagaag cattaaaaag caaaataaat atagaaaatt
    13321 tatcagaaga gatgagagtt ttatatgttg catttacaag agctaaagaa aaattgatta
    13381 taactggttc tactagaaat atacaagata gtataaagag atggtcaaat ggaattgaaa
    13441 gtttagatac aatatcacaa tacgagatat taaaaggtaa aaatttttta gattggataa
    13501 tgccttgtgt attaagacat agggatttat ctaatctact agaagaagta ggattagacg
    13561 cagtttttaa tgttgaacat aattcaaaat ggtatggaaa gttgtggaat aagaatgata
    13621 ttttagtgga aaagaagagt gatgaagaaa aagaaagtat tgaagaaatt cttgaaaaaa
    13681 tagatgtaga tagtccggac agtgattatt acagtgaaat agaaaaaaaa ttaaactata
    13741 tatatccata tgaatttagt acaagaaaac ctgctactat atctgttaca gaaataaaaa
    13801 agatacaaaa taattatgaa gaagagttaa taaatactat atttgaacaa aaagtaatat
    13861 taaaaaagcc tctatttatt caaaatgaag aagaaagaga aaaaataagt ggaacagaaa
    13921 gaggtacaat agtccatttg gttatggagg ttttagactt aaaaaatgta agtagtgtaa
    13981 atgatataaa atcacaaata agaggttttg tttcaaaagg tataataaca gaaaaacaag
    14041 ctagtatagt aaatccatat aaaatatata aattttttgc ctctaacata ggaaaaagaa
    14101 tgttgaatgc tgaaattata aatagagaaa aatcaattta tgcacaagta aatatgaaag
    14161 acatatatat ttatgaaaag cttataaaca atgatgataa aaaactttat gataatgaaa
    14221 gtgtcatgtt aagaggtata gttgatgctt attttgaaga agataatcaa atagttttgg
    14281 tggattataa aacagatttt gtaaatgagg aaaatataaa tcaaataata gagaaatata
    14341 aaaaacagtt ggatttatat gcagatatta ttgaaacttt aactggtaag tcagtaaaag
    14401 agaaatgtat atatttattt ggagttgatg aagctgtttg ttattagttt ttatatataa
    14461 attaaaccta aaagatagag gaaggggaaa aaatgagatt tattcataca tcagattggc
    14521 atctagggaa gtccctagaa ggccattcta gaattgagga acaagcaaag ttttgtgagg
    14581 aatttataaa aatagtagaa tcaaatgaaa tagatatggt tataattgct ggagatgtat
    14641 atgatacttc taatccccca gcacaagcag aaaaactatt ttatcagact gtatcaagac
    14701 ttgccaataa tggtcagagg tgtgtattaa taatttcagg aaatcatgat aatccagaga
    14761 gactttctgc aataacacct ctagcacatg aacaggggat acttatatat ggttatccat
    14821 taagtgctac gatagaagct aagtataaag ggtttgaaat aacatatgca actcagggat
    14881 gtacaaaact gaatataaat ggagaaaata ttgtgattgc tacattacct tatccaagtg
    14941 aaaaaagatt aaatgaagtt ttttcaagtg aagatgagtt tgagaaacaa aaaaattatt
    15001 ctgaaaaggt aggagatata tttagaagtt tagaagaaaa ttttagaagt gatactataa
    15061 atatagccgt atcacatata tttgtaatag gtggagagag tactgaatca gaaagaccta
    15121 tacaattagg tggaagtttt ttagttgaaa gaaaagattt gccagaaaag gctcaatata
    15181 ctgcactagg tcatttacat aaacaacaaa aagcttccga acgtttaaat gcatactatt
    15241 ctggttctcc acttcaatat agcaaggatg aaagagcgta taccaaaggg gcatacatag
    15301 tggatataaa agctggagaa aaaccaataa ttgaagatgt atattttaat aattacaaac
    15361 ctatagaagt atttaaatgt aatggaatag aagaagcact agatatatgt gagcaaaatc
    15421 aagatagaga gatttggtca tattttgaaa taaatactga tgagataata tcacagaatg
    15481 aaattaaaaa aatgaaggaa ctattaaaag acataataga aataaaacca ataataacat
    15541 cttgttatga acaagaaagt gtagatataa aagaaaagtc tatggcagag ttgtttagag
    15601 agttctattc ttttagtaaa ggtgtagagc caaaaggaga acttatggat ttatttctag
    15661 atataataag tgaagaaggt gaatcagcag atgagaccaa ttagattaga acttacagga
    15721 cttaatagct atattgataa acaagttata gactttgaaa aattaattga aagaggtttg
    15781 tttgggatat ttggaactac tggaagtgga aaatctacaa tcttagatgc aataactatt
    15841 gctatgtatg gaaatatatc cagaaataca aaagaatata taaacagtgt gtgcgataag
    15901 gcaattatat catacgagtt tgagatagga agcaaaaaca ctagaagaag atacattgta
    15961 gatagaacca ttgctagaag caaaacagga acaaagacct ctaatgctag attaatagaa
    16021 gtattgaatg acaatatcca aaatgtttta gctgataaag ttgtggaagt aaatgaaaaa
    16081 gtagcacagg ttgtaggatt aacggcaaat gattttacta ggtcagtagt tttaccacaa
    16141 gacaaattta atgagtttct aagattatca cgtgcagata gaagagacat gttagagaga
    16201 atttttaatc ttgagaaata tggaagaagt cttggagaaa aagtgaaaaa gagaaaaaat
    16261 atacagttac aaaatttaaa agatttgaaa tcaaaattaa gtcaatatga tggaattaca
    16321 gaggaagtat acaacaatat aaaccaagaa ttaatagagt taaaaaattt ggaaaaagat
    16381 aagaataatg ctttagacct agctcaaaaa tcttatgaag aaagtaaaac agtatatgaa
    16441 gaacaattaa agttagaaaa aaatgaactt agaaaaaatg agcttgattt aaaaaatagt
    16501 gagataaaag aaaagattgc tcttgtagaa aatgatagta acgctaaaaa ggtagaccca
    16561 tacataagtt cagttcaaaa tctagaaaaa aagatagatg aagatagctt tgtagtaagt
    16621 aatttagaaa agaaattagc aatattaaat caggagcttg aagttacaaa aaatagatat
    16681 gaaaaaataa acaaaattaa gaatgaagaa gtccctaaat tgagtgaaga aaaaataaga
    16741 ttgcaacaag caataaaatt agaagaagag ttagttttat tagatagaga gttaaaagat
    16801 ctaaaagaaa gtggaattaa tttaaataag actaaagttg aattagaaaa agtaaagcag
    16861 gtgtcagagt ccagaaaaga tgcagttact aaatctataa aagaagttga aggaaagatt
    16921 gataaagtaa atataagtgc agaattaaaa caaaaaatat ttctagcata tgaatacgag
    16981 aaagattaca acaacgtatt ggaagaaaaa aatcaaaagc taaataaact agaagaaata
    17041 ctcaaagaga cagaaaatat aaatttaaaa gttagatata ttgataaaga taagaatgat
    17101 gtaaatcgta atttagaaaa tttatcactt cacttggatg ttcttcttaa aaaatgtcct
    17161 ggtaagagtg ctgatttact tttaaaatct gaatatgtta ctgaattaag aagtaaagca
    17221 aataatacta aagaaaatga aattaagaaa agcagtatac aagatgaatt aaagataata
    17281 cttgaaagta agtttaatac tgaaagggaa gtaaatttac taaatgaaaa acttgaaaat
    17341 aatagaaaga atagagatga cctagaaaaa gaattagaag aactaaagta tttaaattta
    17401 gcctctgaac ttagacgtga attaaaagaa aatatgcctt gtccagtttg tgggtcaaaa
    17461 catcatgaaa atcataatat aactaagtat gatgaaaata tatcttttgt caaagaaaaa
    17521 ctggagaaat tagaaaaaga aaaaataagt ataagaaaca atatagagga attaaatgct
    17581 aaagtaagtg ggtatttatc tatagaaaaa atgaagacta aagaacttga agatgtaaaa
    17641 ggaaaactag gggggattcc atctagtcaa ttattaaaaa aacttgatga agaacaaaga
    17701 aaacttgcat tgttaaaatc taatattcag gagtgggaga aagaaaaaga gagtactgaa
    17761 aataaagtaa cattagctaa agaagaaaaa aataaaattg aaaaagaaga attaaaaata
    17821 agagagagtc tgaataatta taagaaatta acaagagatt taaacataga aatagaaggt
    17881 ttagaaaata aatgtaaaaa acttaaacag gaatacctag gattaaagac gattactaaa
    17941 gtctctaatc tattgtcaaa agtaaaagaa ataagggaga atgaaaaaga attagaatta
    18001 ctaagtactg ggcattcaaa tttattaaaa aatagagact cactagatat taaaataaga
    18061 gaaaatgaga gtaaactaca tgaaatagag atagaattaa taaaggctag agaattatat
    18121 gtagaaaaga aattgagtag agataataag tataaagaag ttattaacat aacgaaaggt
    18181 gatttagcta aaaatctttt acataatgtt gaagaaaata tatgtaagat attagaacaa
    18241 gaagaatcaa gtaagaaaaa attggaagag caaagattag aatatgaaaa aaacattgct
    18301 gaaaagcaca atatagatgg tagacttaaa actgctaagg aacaatataa agaccaaaaa
    18361 tcaacactaa ataaactatt agcagaaaat aaatttgaaa gtatatatgc agtaaaaaga
    18421 gctttattag atactgatgc agtaaaaaaa ttgattgaag aaatactaga gcatgaagaa
    18481 gaacaaaagt tattatcatt taagataaaa tcatcaaaag aaaaattaaa tggaagaagc
    18541 ataaagaagg agtactttga acagttaaaa gatgaaattt ataatttgaa agtagaaata
    18601 ggaaatatat ccaaagatat tggagctaat caaaatcagt taataacact aaaagactct
    18661 ttagataaga taaatgattt taataaacaa cttaaggtag ttgaacacaa tgtggactta
    18721 ttggaagaat tagataaatg tgtgcaagga aataaatttg tagaatatgt ttctacaaac
    18781 caattaaaat atatagcatt ggaagcatca aaacgattgg atggaataac aaaaggaaga
    18841 tatgcacttg aaattgactc aacactaaac tttgtaatga gagataactt taatggagga
    18901 gaaagaagaa gcattgatac tctatctggt ggagagacat tcttgacatc tctatcatta
    18961 gcacttgctc tttcatctca aatacaatta aagggaagtg caccattaga atttttcttt
    19021 ttagatgaag gttttggttc attagatagt gaacttttag aaattgttat agaatccttg
    19081 gaaagattgc atagtaataa tctaagtgtg ggtataataa gtcatgtcga ggaacttaaa
    19141 aatagagttc ctgttaagct attagtttca tctagtgagg ctggaatagg ttcaaaagta
    19201 aaaattgaat atagttagaa aatatatggt aattgaatat attatctaat atttccaaat
    19261 aatatgtaat tgtgatataa taaaaactaa tattaaagtt atttataatt aatattaata
    19321 taaaaacatg taagtaggag tgtggtaagt gttggaatcg cccaataatt tgattcagat
    19381 tattttttta atagtattac ttatagggtc tgcatttttc tcagcatctg aaacggcctt
    19441 aatgtcgttg agtaaaatca gaatcagata tatgcaagat gaaggagtta aaggagctaa
    19501 gttagtaagt tcattaatag aaaatccaaa taagctttta agttctatat tggttggaaa
    19561 taacgtcgta aatatagcag caacatccat atcaacatct ttgtttatag gattaatggg
    19621 agaaaaagga gtggcacttg ctacagctgt tatgacagta ttagtattaa tatttggtga
    19681 gattactcca aaaacaatag cagcaaataa ttcagaaaag gtttctctat tagtatctaa
    19741 acctataaaa gcaattatat ttatattaag accaatagta tggatattta atataattac
    19801 aaatattata tttaaattat ttggtataac aaataaagga gctaaatcat tcataactga
    19861 agaagagcta aaaactatgg taaatgtaag tcatgaagaa ggcgtccttg aaatggaaga
    19921 gagagaaatc ataaataatg tttttgaatt tggagatatg caagctaaaa atgcaatggt
    19981 acaaagaata gatatggtag ctatagatat ggaagatagt tatgatgaga taatacaggt
    20041 atttaaaact gaaaaattaa gtagaatgcc tgtgtatgaa gaaactatag atgatattgt
    20101 aggaatactt aatataaaag atattatatt tttatctgat gaagaaatag agtcatttga
    20161 tattaaaaat tatatgagag aacctttttt cacgtatgag ttcaaaaaga taacacaact
    20221 tcttgaagag atgaaacttg aaaaaagtca gatggctata gttgtagatg agtatggtgg
    20281 aacatctgga cttttaacta tagaagattt agtagaagtc attgttggag atatagagga
    20341 tgaatatgat gaagaagagg atgaaataca agttataaaa gaagatgagt atattgtaga
    20401 tggaagtact aaaattggtg atgttaatga acttattggt gtaaacttgg aatctgagga
    20461 gtttgattcc ataggaggtt ttataattgg acacctaagc agattgccag aagaaaatga
    20521 agtaattgaa gttgataata taagattttg tatagaaagt atagaaaaaa atagaattaa
    20581 aaaaataaga atatatacat aatgtaataa tgttc
//
LOCUS       DAGQWR010000077        20559 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.077, whole genome shotgun sequence.
ACCESSION   DAGQWR010000077 DAGQWR010000000
VERSION     DAGQWR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.077"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            375..2468
                     /locus_tag="MIW95_002345"
     CDS             375..2468
                     /locus_tag="MIW95_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY3151667.1"
                     /translation="MNKRNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEQNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTILNKPIKINEFNEISNTKEGKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTQSHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
     repeat_region   3129..3422
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3129..3157
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaat"
     gene            complement(3590..3874)
                     /locus_tag="MIW95_002346"
     CDS             complement(3590..3874)
                     /locus_tag="MIW95_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151668.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     gene            4081..4899
                     /locus_tag="MIW95_002347"
     CDS             4081..4899
                     /locus_tag="MIW95_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY3151669.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            complement(5032..7152)
                     /gene="feoB"
                     /locus_tag="MIW95_002348"
     CDS             complement(5032..7152)
                     /gene="feoB"
                     /locus_tag="MIW95_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBY3151670.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(7178..7405)
                     /locus_tag="MIW95_002349"
     CDS             complement(7178..7405)
                     /locus_tag="MIW95_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBY3151671.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(7732..8172)
                     /locus_tag="MIW95_002350"
     CDS             complement(7732..8172)
                     /locus_tag="MIW95_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3151672.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     INLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            8324..9313
                     /locus_tag="MIW95_002351"
     CDS             8324..9313
                     /locus_tag="MIW95_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBY3151673.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     misc_binding    9465..9717
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9789..10997
                     /locus_tag="MIW95_002352"
     CDS             9789..10997
                     /locus_tag="MIW95_002352"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBY3151674.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     gene            complement(11058..11939)
                     /locus_tag="MIW95_002353"
     CDS             complement(11058..11939)
                     /locus_tag="MIW95_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBY3151675.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPGRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            12325..13269
                     /locus_tag="MIW95_002354"
     CDS             12325..13269
                     /locus_tag="MIW95_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBY3151676.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLI
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            13492..14037
                     /locus_tag="MIW95_002355"
     CDS             13492..14037
                     /locus_tag="MIW95_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBY3151677.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            14128..15276
                     /locus_tag="MIW95_002356"
     CDS             14128..15276
                     /locus_tag="MIW95_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBY3151678.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQNSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSKKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            15329..16594
                     /locus_tag="MIW95_002357"
     CDS             15329..16594
                     /locus_tag="MIW95_002357"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBY3151679.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(16880..18112)
                     /locus_tag="MIW95_002358"
     CDS             complement(16880..18112)
                     /locus_tag="MIW95_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151680.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(18115..18789)
                     /locus_tag="MIW95_002359"
     CDS             complement(18115..18789)
                     /locus_tag="MIW95_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151681.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLVN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            complement(18797..20026)
                     /locus_tag="MIW95_002360"
     CDS             complement(18797..20026)
                     /locus_tag="MIW95_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBY3151682.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            complement(20138..>20559)
                     /locus_tag="MIW95_002361"
     CDS             complement(20138..>20559)
                     /locus_tag="MIW95_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY3151683.1"
                     /translation="NIIKKYSKYFISKKIILDLNNLNFKVLSDEKWLYFIFEQLLSNS
                     LKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDLNRIFNKGFTGNNGRNNSKSTG
                     LGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL"
BASE COUNT         7624 a         2557 c         3147 g         7231 t
ORIGIN      
        1 ttagaaaaat tatgatgttt ttagaaatta atgcttttta acttcaatag tatcaataat
       61 actgattttt tataaaaatt taaaacaata ataaaatata taatttattt ttgtggacaa
      121 tttgtcaaga ttagtaccaa gttaaaattt aatataagag agagatgtat tatgtaatat
      181 taattttttt taaattggta atactatatt gctgtatttt tttttatcaa ttataattta
      241 aaatatgaaa taataaactt tctaattaaa ttaaggtatt tagtgtgtat gtataagtta
      301 taagtaaatc ttctaaaaag aataatttta acagtgttgt tataaagagc agtaactatg
      361 gaggagataa gtatatgaac aaacgtaatt ttgaagttat attaaatcaa ctacagatta
      421 atatatatgt tacaaatatt catactaatg aaattatttt tatgaacaag aaaatgaaag
      481 aagaatataa tattttagat ccagaaggaa aagtttgctg gcaagtatta tatcctgagc
      541 aaaattctac ttgtagtttt tgtaaagttt tagaactatt aaagaatgat aaaaaaggag
      601 tattaataaa gtggtatgaa aaatgtaata agttaaatcg tgtttttgaa aattatgata
      661 gtttaattac atggcaagat ggcacagttg ttcatatgca tcaatcaata gatattgcaa
      721 actcaacaat cttaaataaa ccaattaaga ttaatgaatt taatgaaata tcaaatacta
      781 aagaaggaaa aggagttttt aatttttcaa gagacaattt tgattataat tctacattgc
      841 tttatgatgc tttgattaga ggtacagatg aatatatata catttgtaat atgaaaacag
      901 gcgtatttcg atattcacca tcacaagttg aattatttga cttaccagga gaaattgttg
      961 aaaatccgct tatttactgg aaaaaaattg tacatccaga agactggaat cgtttttata
     1021 agtcaaacac tgaaataggt aaaaaccaga tggattatca tacagtggaa tttagggcta
     1081 aaaatcgtag tggagaatat atatggttaa gatgtagagg acaactaatg cgtgatgaat
     1141 ttggagaacc aagtattttt gcaggaataa tgacgcaatt aggtaaacaa aataagattg
     1201 attcattgac acagttactg aattatcatg aatttatgag tgtatttgaa gataaaattt
     1261 caaatcctat gattgaaaaa ttatgcatag tattattaaa tattgacgat tttaaaaatg
     1321 ttaatgagat gtatgatagg gattttggag ataatataat aaaaacacta gcacagtcta
     1381 tacaatctat tttaccagat aatgctgaac tttataaact tgatggggac gaaatgggta
     1441 ttcttgttga taatgttgaa gaaaatgaaa ttctaactct atataatcaa atacagaata
     1501 tgattataca ccttcaacta tggagaaaat atggattaaa tataacaatt tctgctggtt
     1561 gtgtaatata tccaaaacat ggagatacag taaaagaact ttataaatgc gctagttact
     1621 ctttacaata tgcgaaagag catggtaaaa acaggcttgt ttttttctcc caagaaatat
     1681 taaaaaataa aatgtattca ttagaaatga tgcgggattt aaaggcatca attaatgatg
     1741 attttagggg atttagtctt cgattccagc cacaagtaga tacacaaagt cataaaataa
     1801 taggtgtaga agttctttta aggtggacta atgacaaatg caaagcaatt tctcctttgg
     1861 aatttattcc aatattagaa gaaaatgata tgataaatat tgtaggggct tgggtgcttc
     1921 gaatggcatt acgtactttt aggaaatgga ttgattacta tccttttttt aaagtgagtg
     1981 taaacgtttc agctgttcaa atattagaag acacatttat tgaagatata gtaaaaatta
     2041 tagatgatga aaattttcct tatcaaaatc ttgttttaga attgacagaa agtcatacag
     2101 tacaaaatat gagtatttta cagtttaaat ttaaggcttt acaagattta ggaatttata
     2161 ttgctatgga tgattttggt actggatact cgtctctaga agttttaaaa ttttctccaa
     2221 tagatatagt gaaaattgat agagtatttg ttaaagatat attaaagagt aagtttgatg
     2281 ctacatttat acattttatt gttgcaattt gtcatgatgt aggaataaaa gtatgtttag
     2341 aaggtgtaga aacacaagaa gagtatgatt tagttaaaca aataaagcca gattacatac
     2401 aaggatattt atttggaaaa ccgcagacag caacagaaat ctttgacctt ttaaagctag
     2461 ataattaatt ctaatttatc aataatatca ctgaaaagct ttaaaatcaa aaatacatta
     2521 aattattata ttgaagatat atcaattata aattgcaata taagaaaata gcatttcaag
     2581 ggatgttaaa ttaggagtag tatatgatta aagtatttta aaaagggata actataataa
     2641 aaatagatat ctatttttag attaaaaata atatatcata aataaaataa taagaggtag
     2701 atacagtttt aagggaatac aaaagttttt aattaaacta tgcttgttca gatagatatt
     2761 tatttaagaa aaaagactat taaaagcaat atacaagaat gatatattag attgattaaa
     2821 caagcataaa tattatgtaa aaaactttaa gttatagaat ttaaatccaa tgtagataga
     2881 ttacgttttt tcttgctttt attatggtat aaattggtat caatattcaa aagtaatata
     2941 tttatgatat aataaaatta tagaaatttt gcagtgagct atatttgtga taaaatttgg
     3001 cgtaacagtt gaaatataag gtgttgagag tgcatgataa gcgttatcaa ttgcactatt
     3061 gctcgttcac tgcaaattta ggagagttgt atacgtgtaa gtgttgaaaa tactaagttt
     3121 attttggggt tttatattaa ctatatggaa tgtaaatctc ggagaatttc ctaatgtgga
     3181 tttaatagga agtgttttat attaactata tggaatgtaa attttagcaa atttttcgta
     3241 aaagtcacta ttttcaatgg ttttatatta actatatgga atgtaaatac atataaaact
     3301 ttcatcttct gttaattcat taaaacgttt tatattaact atatggaatg taaatccatc
     3361 ttttgttgtt ttacataaat ttatattact tacgttttat actaactata cggaatataa
     3421 atttttataa ttttctttta atgtgttatc ttatgattat ctagttatat tagcatacaa
     3481 aaatataata aaattacact atattataaa aaccaaaagg tagtatagaa atcctattac
     3541 ctttttatta ttaattttat cagtgtttta ttataaatag cttacataat tacacatttt
     3601 ttctgattaa ataatatgat gctattgtta taatagatac taatgctaat gatgttattg
     3661 cagtatctaa tcttcttatt aataaactca tttctatata ttcaagttct attaaatatt
     3721 gataaaatat ttctagttta acagcttttt ctctatctat atttccatac tttaactcta
     3781 atttatctaa agatttttta ttacaataat taactttatg tttaattaaa tatcttattg
     3841 tagcacctat tacaattttc actctaacaa gcataaatat aatattccat ccaaaagtta
     3901 agaggggata tcttttttat gagaggaatg gataaaatat ttataactga catctaactg
     3961 atatttaagc tttaaaatca aaaccatatt gattttctaa aataataact acttgattta
     4021 gtaaataata aagaatatag taactatata gacaacaaaa agaaaaggag tgtataaccc
     4081 ttggaaaaac agtattttac aacaggtgaa tttgctaaat tgtgtggtat atctaagcag
     4141 acattaattt tctatgacaa aataggtatt ttttctcctg aatataaaga taaaaataat
     4201 tatagatatt actcagttta tcaatatgat acacttgata tattacaatc attaagagaa
     4261 ataggaatga gtttagagga aataaaagag tatatacaaa atagaactcc ccagctttgt
     4321 gtgaaaatgc ttaaagaaga agaaaaaaag ataagagaaa aaataaaaaa actaagaaag
     4381 ataagctcta agatgcaaaa tagaataaat ataacagttg aaggaatttc taaaatgaat
     4441 agtgaagaaa tttatatatc caaaaagttt gaagaatatt tagttctatc aagcgatttg
     4501 agtaatatga gtaatagtga ttttatgatg gaattaatag atttcaccaa ttattgtaaa
     4561 tcgaaaaatt tgtatcaagg atatgagttg ggagtaatag taagtaatga aaatataaaa
     4621 aatggggact atttaagtat atcaagtttt tatttgaaga tagataaaaa aataaaggat
     4681 aaaaagctat atgtaaagcc agaaggaatg tatgcttgca taaaacatat tggaaaatat
     4741 gaggattcat ataaatctta cgaaaaattg aaaaaatata tatatgaaaa tgaatataaa
     4801 attattggaa attcgtatga ggagagtata cttgactttt tttgtgaaag taatgaagat
     4861 aattatatga ctgaaatttc aattcaagta tccagataag aattaaaata gagatatatt
     4921 caaatgttaa gtaattttac ttatcattga atatatctct atgctttata ataaagtata
     4981 taaattttta aatagtttct ctaatatatg tctaatgtca gttaatacta attatataaa
     5041 taaattgtaa actgtagttg ttaaaaaaca aacaacaaaa gcaagtatag taggcattaa
     5101 gaatcctaag gcagtccatt ttaaactacc agtttctttt tttattgtta aaagtgttgt
     5161 tgcacaaggc caatgcagta aactgaacag cataacattt aaagctgtca aataagtcca
     5221 tccatgttct ctaagaactt gacctaaagc actaaagcta tctaattcta tcatagaacc
     5281 agttgctagg taagccatta aaagtatagg tacaactatc tcatttgctg ggaatccaag
     5341 aataaaagca agtaatataa aaccatcaag accaattaat ttagctaaag ggtctaaaaa
     5401 gtttgcaaca tgtgataata tgcttaaatc tcctatatat atatttgcaa atatccaagt
     5461 tatgacacca gcaggtatgg ctaccattac agcccttcct aaaacaaata ttgttctatc
     5521 tattatagag gtatataaag ttcttcctat ttgaggaact ctataaggag gaagttctaa
     5581 tgtaaatgta gacggaactc cttttaatag cgtcttagaa agagtataag aaaccaacaa
     5641 tgttattata acacctagaa ttatcagtaa agttatgcaa agtgcagttg ctacgcttga
     5701 tacgaaagag ttggttataa ctgagctaaa aaatattgta gatatagcta tcaaagtagg
     5761 aaaacgacca ttacaaggaa caaagttgtt tgtaagtata gctataagtc tttcccttgg
     5821 agagtcaatt atcctacaac caataactcc agctgcatta catccaaatc ccatacacat
     5881 agtcaaacac tgttttccat gtgcacaagc ttttttaaat aggtggtcta agttaaatgc
     5941 aactcttggc aaatatccaa aatcctcaag tagggtaaat aatggaaaaa atattgccat
     6001 aggaggtagc attacagaga ttacccatgc taatgttctg tataatccta aaaccaacat
     6061 atcatttaac catgatggac aattaatact atttaatata cccgaaatac ttggctcaaa
     6121 tccgagtaat aaattagata ataaagtgga tggatagtta gccccttcta tagtaatcca
     6181 caatatagta cctaataata agagcattat tggaagccca aatattttag atgttaatat
     6241 tttatctact ttttcttctc tatcagttga ttttttagca acattagaac aacactcatc
     6301 actcagtttt ttagcataat catagttgat tttagtaaac tcatctctta ttttctgttt
     6361 atttatattg tctggaatct ttttctttac ttcattaata gcatctatag aatctttgtc
     6421 tatataatta ctcatagatt caaatatact ttcatcgcca tcaataagtc taagtcccaa
     6481 ccatctagaa tttatacctg gtataatatt atccaattct ggttgaattg attttaccac
     6541 attctctata ttttcattgt atctaacagg tttattattt aatttgtatt tgtcaaaaga
     6601 tacatcattt aaagtatcca atagttcatc cataccagag ccatttcttg cagcagttaa
     6661 aataactgga ataccaagag aatcctctaa aagtttttta tcaatagtaa tgcctttttt
     6721 tctagcttca tctattaaat taatacacaa aataacttta tctgtaagtt ccatcacttg
     6781 aaatactaaa tttaaatttc tttcaagaca tgtagcatca catactacta ttactgcatc
     6841 tggattacca aaacatataa aatcacgagc tacaatttct tcttgtgata aggcaaataa
     6901 agaatatgtt cctggtaaat ctattaatgc atattcggta tttttatatt taaaattacc
     6961 acgagctgta gctacagttt ttcctggcca gtttccagta tgctgtctaa gccctgtaag
     7021 atgattaaat acagtacttt tacctgtatt tggattgcca gccaatgcaa ttacaaactg
     7081 gtcttcctta ttgtctatat caaacatatc ttttaaagaa ctcattttag ttgagttatg
     7141 tgtcaacccc atattaatat cctcctgcta gaaattttta aatgtcagaa actaatatta
     7201 agctactttc ttcttgtcgt agagctatct tactacctct gattttatag acagttaaat
     7261 tattttttgg accttttcta actacatcta taacagctcc tctagtaaga ccaagcgcta
     7321 acattctttc tcttaaatta ccactagata atatatcttc aactttgaca gtttttttca
     7381 cttgaatatc gttaaggttt ttcatagtag cacctccatg cttgaaattt agcctgggct
     7441 aatcttctta ttctaaacta tgcattatat tattaccttg ttacttttac aaaaaaaaga
     7501 taggcaagat gcctatcata caaagttggg tataattctt acttttgggg aaagtagaat
     7561 atagaataaa aattaacact ttgaatccat ataattattg aattttgata ataaaaaagt
     7621 cagaaagcaa aagcctcctg actctatgta aggtattttc aaatacctaa atatcaattc
     7681 aatatttaat ttatacttaa attatatatt aacaatatta gcttgtcaac actataatct
     7741 cattttaata tgttcaatac ctgcttcgtc gtatacttct gagatttctt taaaaccaca
     7801 agataagtaa aggttcttta tataagtttg tgctgataag gttatattat tttcgtgtaa
     7861 gttaattttt ataaaatcta tagcacaatc catcatttgt tttgctaatc cttttcttct
     7921 atgactagaa agaactaaaa ctctaccaat ggaaacatca ttataagagc tgtattcttt
     7981 aggaattatt ctagcataag cacaaatcaa tccattttct tttaaaaata tgtgataaga
     8041 gcttttatct atatcatcat aatcgtttaa tgaaaatatc ttctgttcac aagcaaatac
     8101 ctcatatctt gattttgcta tttcataaaa ttcatctaaa ctcaaatcat taaagtgttt
     8161 aattttaaac atagtaatta cctcctagca tcttaaaaaa ctgaatatta agatacatta
     8221 taccattttt aacataaata cctttattaa aatttatatt tattaaaaat atttttaaaa
     8281 agtgatatac tatattaaaa acaaaacttt agttagaaaa tttatgattt atggaggcag
     8341 tgttttgaat actactaaaa aattaactga agcagcttta ttgtcatctt tattcatagt
     8401 agtaacaatt atagctgtga gtactggatt tggatatgct atttacttag attttatagt
     8461 gccagtattt ttttgtataa tctgcttgaa atgtgattta aagtatacca ttttatctgg
     8521 aattacttca cttttaataa tttctctggt acttggaaat ctaggaactg ctatttgggc
     8581 aagtcagagt gtattacttg gtataatgtg tggctatcta ataaataagc ctacaatggt
     8641 tatggatgat ttagtatatg gttcagtatt tggggttatt gtaatggttt tcattgatat
     8701 atatgcttca acattaatag gttatagttt tatgaaagaa tttcaaggat atagcaaatg
     8761 gatttatttt aatgggtata caaattttat ttattattta atgatagcat tgtttccttt
     8821 tggtatggtg ttttgcatat atttattaag tttaatttta gggaataaat tgcatatttt
     8881 agattctaat tctttaaaaa aatttttaat atttaagaat tttagaagtt taaaccaatt
     8941 tttatgttgc tcaaaaaaag ctttttacat atgtgtttct tatctagcca tttttgaagt
     9001 tctaaaactg ttgaatgtta aagttgattc tgtttattta aaaacaatct ttatatcaat
     9061 aacctatata agtgtctatt ttatcattag agactcatgt atgtcactac aaaactttat
     9121 aatagctaag tttagaaaat tattatatgc tagaatttta tttttgataa taattttatt
     9181 gctgtttttt atatttgatg taacagttat tagtttaata attataaacg catttttaaa
     9241 taagaaaata aatataagat tgagtcagag taatatagtc aataaacaaa taaatctttt
     9301 gattgaaaaa taaactttgg gtattggtaa aaaatataaa aaagtagtat aatgtaaaaa
     9361 aacatgtata taataataaa aaaggttgac aaaatataaa aataataata tattatgagt
     9421 aagttaagaa aaatttcttg taattgatga aataatctga atatataaat gctatgaaga
     9481 gaagaagtag ggctagattt tatttaacag agagttccag tttggtgaaa tggaataaat
     9541 tgatttagtt tgaatacatc tcagagcact atctctgaaa gataacaacc attaagagat
     9601 atcggattag tacacgttat agtacacgag tatatgtaat tgtactctaa gagattaata
     9661 tcgtgagata ttgatgaact aaggtggtaa cacgtaagca atgctttcgt ccttttaaga
     9721 ggatgaaagc tttttttatt ataaaaaaaa aattatagat tatataaata ttttaggagg
     9781 aatgaaaaat gaaatcgata gacgagcaaa tgcgaataat tatgaaaggt gtagatgatt
     9841 taatagatga gaaagagtta agggaaaagc ttattaaatc agaaaaagaa ggaaagccta
     9901 tgatagtaaa attgggtcta gacccaagtg ctccagatat acatttaggg catacagtag
     9961 ttcttagaaa gatgaaacaa ttacaagatt taggtcatca aatagtaata ataattggag
    10021 attttacagg aaaaattgga gacccaactg gaaaatctaa agctagaaaa gctttaacaa
    10081 cagagcaagt tcttgcaaat gcaaaaactt atgaagaaca aattttcaaa gttttagata
    10141 aagaaaaaac tattgtaaga tttaatagcg agtggttagc caagttaaat tttgaagatg
    10201 taataaaatt agcagcaact attactgttg cgagaatgtt agaaagagaa gattttaaaa
    10261 agagatatga aggacaaatg ccaatatcag tacatgagtt cttctaccca ttaatgcaag
    10321 catatgactc tatagcatta gaagctgata tagaacttgg tggaactgac caaagattca
    10381 atttactaat gggaagatca ttacaaagag aatttggtat ggagtctcaa atagtaataa
    10441 tgatgccttt aatagaagga ttagatggta aagaaaaaat gagtaagagt ttaggtaact
    10501 atattggaat agatgaagaa gcgggaataa tgtaccaaaa atctatggaa attcctgatg
    10561 aacttataat taaatactac aatttagtga cagatgttca tcctgatgaa gttaataaaa
    10621 tagaatctca gttgaaagag ggttcagtaa atccaagaga tataaagatg aacttagcaa
    10681 gagaaatagt aacattatac catggagaag aatctgctaa agaagctgaa gaaagattta
    10741 aatctgtatt ccaaaaaggt caaatacctg aagatataca aacaattcaa gttaaggaag
    10801 atggatttga cttaatcgaa gtactagttt ctaatgagat tgttaagagt aagagtgaag
    10861 ttagaagatt agcttctcaa ggtggagtaa aggtaaatgg tgaaaaagta gaggatttaa
    10921 gcactattgt aaaagagagt gaattagttg ttcaaatagg aaagaaaaaa tttgtaaaaa
    10981 tcgagctagt aaaatagagt aaagtaaaag caagtctatt gagtataaaa tgtacattca
    11041 ataaacttgc ttttttatta tattaaaatt ttaaattgat aaccattgtc tttaaaatat
    11101 gttataattt ggtccaatgc atttactgtt tcttgttttc cataagtatc atgcattaaa
    11161 agaacaacca tttctttgcc ttcagaagat ttctttgcat gttcaaataa ttcttgtgca
    11221 tttttctttt taccttctgc atcagcattt aaagcgttcc aatcaattga tgccatgttt
    11281 ttctctttta agtagtttcc tagaggttcc atatttttcc atgacatata cccgcctggg
    11341 catctaacta catttgagga aaaattcttt cctaagactt ttttcatagc ttcatcagtt
    11401 ttgttaagtt catttacgaa tgtatcgaga tttaggcttc tgccaggata tagtttttta
    11461 tagtcatggg tataagagtg atgggctatt gcatttcctt cgtcaaatgt tctttttaaa
    11521 atttcattag ctccttttct ttcaagagaa tcacccttaa taaagaaagt tcctctaaca
    11581 ccatgtcttt tcaagatatc tagaacttgt ggagtattag tagtagatgg tccatcatca
    11641 aatgtaagaa agactatctt ttctccattg tttgaatagt cgtatttgtt aagtttgtcc
    11701 caaactattt ttgcgtcata tccatattct tttacagctt ctttttttgc accaataata
    11761 ggttcatttt tttctttttc aattctttct ttttctaatc tttcagcttt aagtctttct
    11821 tgagtttctt tcatttcttt ttttctagac atgtccgccc atgccataga aaaaatacca
    11881 ataactatta caacaagtat agtaagtgta acagttttct tttttctttt ttttaccata
    11941 attacagcat tccccctttt gtcgaattgt atcaaaaata tattataatt atatcgaaga
    12001 aagtagaaaa agtaaaccga caaagttgta actaagataa ctaaagtgta acttatatga
    12061 cattatttgc ataatattaa agtatcatat atattaaaaa tatctataaa aaaatttagt
    12121 ataggtaaaa aaatataaaa aatataaaaa tttattaaaa aggtgtttac aaaaaatgat
    12181 taatatatta ctgttaaata gtggatataa atttgagctc aaaatgtatg taaatttaga
    12241 tttaaaatac tgaaaaaatt tactatatta atttatagta atgcttatag aaaaactctc
    12301 ttgctgttta gggggtttat aattttgcaa aaaaataaaa aggagggggc tttattgaaa
    12361 aaatactttg gagaaatagg actaatattt atagcaatta tatggggaag tggctttgta
    12421 gcaacccaat ttgcattaga cggtgggctt acacctctac aaatcataac tttaagattt
    12481 ttcttggcag ctataattat gaatttactg ttttttaaac agattcgtgc caatatgggt
    12541 aaaaaattat taaaagcagg agggatttta ggaatatttt tatttctagc attcacagta
    12601 caaactattg gacttatgta cactacacca tctaaaaatg catttataac agcagctaat
    12661 gttgtaatag ttccgttcat aggatttata ctttatagaa gaaaacttga taaaatagga
    12721 ataataagta gtttggtagc tcttatagga attgggatac tttctttaga agctgatttc
    12781 tctattaatt ttggagattt tctaacactt atatgttcat tcggatttgc atttcatata
    12841 ttctttacaa gtgagtttgc aaaagataac aacccaatgg cattgacagc tattcaattt
    12901 acagtagcgt ttttaatgtc tgttgtagtt caaacatttg caggtcaatt aaagatggaa
    12961 gctgaattat ctggttatat gggaactatg tatctagctg tatttagtac tacaattgga
    13021 tttttatttc agactatatg tcaaaaaaga gttgatggaa ctagaacagc tataatatta
    13081 tccacagagg ctgtatttgg tacaatattt tctataataa ttctaaagga acttattaca
    13141 gcaaaattga ttattggtag tatcttgata tttgttgcaa tcataactgc agaaactaaa
    13201 ttatcttttt taaagagcaa aaaagtgaaa cttaaagatt cagaggagtc ttcattagaa
    13261 agtatttaat atgacaacta tttcctaatt aatcaaataa gttatgtatt atgtagaaat
    13321 ttgacatcaa aacttggttg atactggtgt ttcaaagtga taaaattaat taggaaataa
    13381 attttatttt tagtgtttaa gctataaagt ctggggtata tatattcttg tagcaagtta
    13441 ttaaacttaa aaataaaaat ttaattatat aaaattggga ggaaattaat tatgaaaaaa
    13501 tttgtttgta cagtatgtgg atatatacat gaaggagatg ctgcaccagc acaatgtcca
    13561 gtatgtaaag ttggagctga taaatttgaa gaaatgaaag gcgaaatggt ttgggctgat
    13621 gaacatagaa taggagtagc tcaaggtgta gatgcagaaa taatcgaagg attaagagct
    13681 aactttactg gtgagtgtac agaagtagga atgtatttag caatgagtag acaagctgat
    13741 agagaaggtt atccagaagt agctgaagca tataagagaa tagcttttga agaggctgaa
    13801 catgctgcta aatttgcaga acttcttgga gaagttgtag ttgcagatac aaaagaaaac
    13861 ttaagagtta gagttgatgc tgagtatggt gcaactgatg gaaaattaaa attagctaag
    13921 agagctaaag aattaggatt agatgctata catgatacag tacatgaaat gtgtaaagac
    13981 gaagctagac atggtaaagc attcttagga ttattaaaca gacattttgg aaaataataa
    14041 ttaaaacata gattaaaagg agaaactttg gtttctcctt ttttaaatta tacaaataaa
    14101 ctgttgtgat agaaggatgg agataatatg aagtcggtat ctgttaggag ttgtaaaact
    14161 tacaattatg aagaagtaaa gttatctatt gaaaaaaatt tagaggatat tggtggtata
    14221 gataagtttg ttaaaaaagg aagcaaagta cttataaaac caaatttatt aatgaaaaaa
    14281 aagcctgaag aagctacaac tacacatcct atggttgtaa aagttgtatg tgaaaaactt
    14341 ttagagttaa attgtgatat tacaatagca gatagccctg gtggtccata taatcaaaat
    14401 tctcttaaga gcatttataa ggcatctgga attgaagaag tagcaaatga actgggcatt
    14461 aagttaaatt atgatgtttc agatgttaag atacatagta aaaaagcaca tgttctaaaa
    14521 tatatggata ttattaaacc aatagtagat tcagattata taataaatct ttgtaaatta
    14581 aagactcata taatggcaac ctttactggt ggtacaaaaa acttatatgg atgcatagca
    14641 ggattgaaaa aagcagagat acattataga tttcctacag aagagttgtt ttgcgaaaat
    14701 gtacttttag atatttgtga ttatataaaa ccaacattga caattatgga tggtatagta
    14761 ggtatggaag gtgatggacc ttcagctggt actactcgag aaataggagt tttattagca
    14821 tcacaaaatc catatgctat agatgttgtt gcatgtaaga ttataagttt acttccaaat
    14881 agagttgcta caattagagg tgctattaag agaggctata tacaagaaga tttttcagat
    14941 attgaaattt taggtgaaga tataaaagat ttaattataa aaaattacaa gattccaaat
    15001 gtaagtaagg atttaagatt gttttccgta aagctaccaa aatttttaaa tgaacctatt
    15061 tcaaagctta taacaccaaa gcctgtggta agatttcaag attgcataaa atgtggaaaa
    15121 tgcaaggaag cttgtcctgc atccactata gagataggga ctaaaggtgc tattatagac
    15181 ttaagtaaat gtataagatg ttactgttgc catgaacttt gtcctaaaaa ggctattgat
    15241 gtcaaacgta attttgtatt taagtatgta aaataatgta taatattata tgagtctata
    15301 taaattaaaa atataaagga gaaataatat gattttaatt aaaaatggta gagtaataga
    15361 ccctaaaagt caaagagatg agaatattga cttaattata aaagaaaata aaatttataa
    15421 aataggcaag tttgatgaaa gtgatgaata tgaaaaaata atagatgcta gtggtaatgt
    15481 agttgctcca ggtttagtgg atgtacatgt acactttaga gaccctgggt ttacatataa
    15541 agaagatata gaaagtggag ctaaatctgc tgctagaggt ggatttacaa ctgttatatg
    15601 tatggcaaat actaatccta ttgtagataa tgaagatact tttaattatg ttaaagaaaa
    15661 gtcaaaaaat gcatgtataa atgttttaca agcagcagct ataactaaag gttttgaagg
    15721 taaagagtta gttgatatgg aagctcttaa aaaagctggt gtaccagggt ttactgatga
    15781 tggattacct ttaatggata gcaatctaat tatggaggca atgattaagg caaaagagtt
    15841 ggatgtacca cttagttttc atgaagaaga cccttcttta gttggaaatc caggtgtaaa
    15901 tgcaggaaag gtagcttcag aactaggatt aaaaggtgca tcaagtgtag cagaagatgt
    15961 tatggtagcc agagattgta tgttggcact aaagactggt gctaaagttg atatacaaca
    16021 tataagttca ggtgtatctg ttgatatggt taggtttgca aaatacttag gtgcaaatgt
    16081 agttgcagaa gcttcaccac atcactttac attgactgag gaagatgtat tggagtttgg
    16141 aacaaatgca aaaatgaatc cacctctaag aagtaaatgg gacagagata agattataga
    16201 gggattaaat gatggtacta ttgaaataat agcaacggac catgcacctc atagtaaaga
    16261 agaaaaagat agagagttta taaaagctcc aagtggaatt attgggcttg aaacttcttt
    16321 agctcttgga ataacaaatt tggtgcataa aaatcatctt agtatgatgc agttaataga
    16381 aaaaatgtct ataaatcctg caaagttgta caatttaaat attggattta tagaagaagg
    16441 ggctgtagca gacattgtta tattcaatcc agaagaagaa tggacagttg aaagctttgt
    16501 ttctaaagct gataattcac cgtttaaagg taaatcttta tatggaaagg ttaattatac
    16561 aatatgtaat ggggagattg tatataacgc ctaatttaag attatttttt taaggcggag
    16621 tgtacaatga ttgaaagtaa aagattgtat atcagcagaa tgagattgat taatcaaaat
    16681 tggaagtatt agtttgaaca attagaacat aacatcttgg taatttagat atagtaagga
    16741 agagaatcct ttttatgaaa gaacgcacta ttatatttgg aaagattaag ttcctaatct
    16801 atcatatata atagtgcgtt atatatttaa gtatgtttta tataaactaa tctaaaaatc
    16861 gaaaatcttt atacaatttc tacttataaa ttgcctttat agggtctagt ttagaagctt
    16921 taaaggctgg aatcagacca aatacaactc cagtcaatat agtagctaca attgcataaa
    16981 ataagctatt taagctaggt atagcttcaa aacctatata tttagaaaca tagttagtag
    17041 cagcaaatcc aactatagtt cctaaaattc caccacatac tgttataaaa acagcttcaa
    17101 ctaaaaattg aaataatata gacctaggtt ttgctccaat agcacgtctt ataccaattt
    17161 ctctttgtct ttccataaca gatacataca ttatattcat tacaccaata ccaccaacaa
    17221 acattgcaac tacagttatt atagatacat atttgtttac attggaatta atgctttcaa
    17281 gataagctgt ctgttcagtt gggtctggtg tagtataaga accattgata ccagggtgca
    17341 tctcatataa tttagctata acattgtttg caacttcatt tacattatag cctttagaag
    17401 aaactaagtc taactggtaa atttcatttg aataagaatt ttgactcatt aaagtgtcaa
    17461 aagccttttt aggtactagg gaagtagtaa attgcatatc ttggtaacct ccaaagaatc
    17521 ctccagcttg gttttgctga gaatcatcta atacccctat tatttcaaat atagttccat
    17581 ttatgttgat accatgacct aaggcatctt ctggattttc aaaaatttca cttgtacttt
    17641 gtaatgtaag taaaattact tttctttttt catcatctaa agaaaaatct ctaccacaaa
    17701 ttaaatttat tttagaatct ttttttactg gtccaacatc aacatacgta gtttttttat
    17761 caaaagatgc ttgtgaagag taaactgaat caaggttaaa cccatctctt gaaggagcta
    17821 ttctttctac tccttcaaca aatgataatt cttctaaatc ttttgcatta aagggtttta
    17881 aaaaaatact catatcatca gtaagacctg tgttatcggc tgattcaaac gaaatagtag
    17941 ttttattagg gtttacattg tttacagatt tcttaatttc tttttgaaat ccattaccta
    18001 tagaactaac aagtattact gaagttattc ctattataat ccagagtaaa gctacaaata
    18061 cacgaagctt gtgacctttt aaattggcca aagaattttt taataaactc atgattacac
    18121 ctccgaagta aacacaccat ctttaagtcg tataacctta gttgcatact tagttaaatc
    18181 ttgatcatgt gtaaccatta ttatagtctt accttgtttg ttgagccttg ttaaaagctc
    18241 cataatttct ctgctagttt cactatctaa tgccccagtc ggttcatcag caaagattat
    18301 ctgagggtca tttactaggc aacgtgcaat ggctacacgc tgttgttgac caccagataa
    18361 ttgaagtggt ttctgcttaa gcttgtccaa aagcccaaca gaacttaatt tatctttaac
    18421 aatttcttct ctattagatt tttttagtgc tttattgtaa attagaggca actctacatt
    18481 ttgatataca tttagagttt ctattaaatt aaattgttgg aaaacaaatc caatattaat
    18541 atttctaaaa acagaacgtt cattttcact caaattagtt acatctgttc catcaaatat
    18601 atatcttcct tcatcaaata catctagaaa acctaagata ttaagtaaag tagttttacc
    18661 actaccagac ttacccatta tcatgacaaa ttctccagat tcaatttcta aatttaagga
    18721 ttttaaaaca tgtaattcat ctttccctac tttgtaatat ttttgaatat tttctaattt
    18781 aattaacata tgtaaattaa ttcacatcca tagtaacttc ttctactttt ttgtttggag
    18841 tagtatctcc attattagaa ccagcagtta cgccatcgcc ttgaactggg tctccttctt
    18901 tcatttcttt agtagggtgt tttattatta tatcgccttg ttcaagaccg cctctaacta
    18961 cagcaaaatc atcattttga cttgctacat ctacaatttg ctttttcaat ataccatcta
    19021 aatccttaaa tacatatgag tgtttgttgt ctttaagtac acaagatgct ggtattttaa
    19081 agcttgaatt agctacttca agtgatgctt gaagatggaa tccatttact aatccttctt
    19141 gattatcaaa tgctatgttt atatcataat aagatagtgt attctgctga gctcccattt
    19201 cagtatttgg agtagatggt ttatcagata taaaacttat tctaccagta agcttttggt
    19261 cagttgaaaa aactagtata ttgaccattt ggtcaatctt cattttaggt aaatcttgtt
    19321 cacttgcttg tcctttcata taaaactcta gaccttgaat agtcataaat gaaactggtt
    19381 ggtctggatt tccagtttgg tcattcaagt atactgtgcc atcaaaagga gcagtaactc
    19441 tgtcataagc ttttttatcc agagaagcta tctgagagtt taaagcagtt atctgtgcat
    19501 ttatagaagc aatagcatct ttattttcag caatatcaga aagtgatatt ttttgttttt
    19561 tgtattgact aagttgtgat tttaaactat ctatttctgc tattatagtt ggatttttag
    19621 cagtaaaaag aaggtctcct tgatttactt tttgaccatt tgtaacatta acatcactta
    19681 actcataatc tccagatata ttgaagtcct tagtttgctt aggaacaacc atcccattta
    19741 taaatatttt ttcattttca ggaatagtat atgtttcgat aaaattttca ttagcttgta
    19801 attcagtgtt tttcgaatca acataagcaa gaactcctag aattaaaagt atgattactg
    19861 ctattcctgc atatattttg aatctcttat ttttaaaggg attgttttta aaacctttat
    19921 ttttagactt gaagccacta cttccaaaac tgctatttct aaagttgtta cttttaaaac
    19981 tgttatttct aaatctttta tttttaaatt tttttagaaa agacatgtct tcctcctttt
    20041 tataaaaact acttaattat atactgggat gtacatatag aattactata agaagttgaa
    20101 tataagttaa tgaaaataaa ctatcaataa taagatacta taaatcacat ttgaatgtaa
    20161 tagtaaattt tgtatattgg ttgtactctg aatctacatc aattttatga ccaagcttat
    20221 cacaaagttc ttttactaaa taaagcccaa gacctgtaga tttagaattg tttcttccat
    20281 tattacctgt aaatccctta ttgaatattc gatttaaatc ttcctttttt ataccacaac
    20341 cagtatcttc cactcttaat tgtaaataat ttttggttct tttgcaatag atagatatat
    20401 tatctcctaa tgaggagtat tttagggaat tactcaataa ttgctcgaat atgaagtata
    20461 gccatttttc atcagataaa accttaaaat tcaaattatt taagtctaaa ataatttttt
    20521 ttgaaataaa atattttgag tattttttta taatatttc
//
LOCUS       DAGQWR010000078        20122 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.078, whole genome shotgun sequence.
ACCESSION   DAGQWR010000078 DAGQWR010000000
VERSION     DAGQWR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.078"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..466)
                     /locus_tag="MIW95_002362"
     CDS             complement(<1..466)
                     /locus_tag="MIW95_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBY3151684.1"
                     /translation="MPNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTISGILMFILGVSYFIIIKSLIEVLSSSEHSLFVKDNVKRFRIIGYLLLLNSIMEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDN"
     gene            complement(761..1465)
                     /locus_tag="MIW95_002363"
     CDS             complement(761..1465)
                     /locus_tag="MIW95_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBY3151685.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVIICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            complement(1819..2835)
                     /locus_tag="MIW95_002364"
     CDS             complement(1819..2835)
                     /locus_tag="MIW95_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBY3151686.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            complement(2963..3469)
                     /locus_tag="MIW95_002365"
     CDS             complement(2963..3469)
                     /locus_tag="MIW95_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBY3151687.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            complement(3628..4296)
                     /locus_tag="MIW95_002366"
     CDS             complement(3628..4296)
                     /locus_tag="MIW95_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151688.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWENDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            complement(4709..5215)
                     /locus_tag="MIW95_002367"
     CDS             complement(4709..5215)
                     /locus_tag="MIW95_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBY3151689.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            complement(5586..6614)
                     /locus_tag="MIW95_002368"
     CDS             complement(5586..6614)
                     /locus_tag="MIW95_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBY3151690.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDINKKMDTIDKLKDI
                     EEGYFYLLEDVYLDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            complement(6662..7522)
                     /locus_tag="MIW95_002369"
     CDS             complement(6662..7522)
                     /locus_tag="MIW95_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBY3151691.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKSI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            7801..8010
                     /locus_tag="MIW95_002370"
     CDS             7801..8010
                     /locus_tag="MIW95_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBY3151692.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            8010..8276
                     /locus_tag="MIW95_002371"
     CDS             8010..8276
                     /locus_tag="MIW95_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBY3151693.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            8305..8877
                     /locus_tag="MIW95_002372"
     CDS             8305..8877
                     /locus_tag="MIW95_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBY3151694.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(9111..10301)
                     /locus_tag="MIW95_002373"
     CDS             complement(9111..10301)
                     /locus_tag="MIW95_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBY3151695.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENVENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(10561..10992)
                     /gene="dut"
                     /locus_tag="MIW95_002374"
     CDS             complement(10561..10992)
                     /gene="dut"
                     /locus_tag="MIW95_002374"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBY3151696.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            11105..13333
                     /locus_tag="MIW95_002375"
     CDS             11105..13333
                     /locus_tag="MIW95_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBY3151697.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEYEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(13444..13929)
                     /locus_tag="MIW95_002376"
     CDS             complement(13444..13929)
                     /locus_tag="MIW95_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY3151698.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            14181..15404
                     /locus_tag="MIW95_002377"
     CDS             14181..15404
                     /locus_tag="MIW95_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBY3151699.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTKESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNANNKFKAGMSAQVNLSIEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            15408..18542
                     /locus_tag="MIW95_002378"
     CDS             15408..18542
                     /locus_tag="MIW95_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBY3151700.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            18576..19874
                     /locus_tag="MIW95_002379"
     CDS             18576..19874
                     /locus_tag="MIW95_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBY3151701.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINNVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNQDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
BASE COUNT         7229 a         3039 c         2610 g         7244 t
ORIGIN      
        1 cattatcctc ttttatcttt attgccttta caaatccatc tgcaattaca aaactcataa
       61 gagaagttat aaaatataca aatactggaa cagtaaaata aaatccaaat cctaaatcta
      121 ggaatctcat tccttttcca gtagtaccaa aagttgaaat aaactccatt atagagttta
      181 aaagcaaaag atatccaata atcctaaatc tttttacatt atcttttaca aataagctat
      241 gttcagaact acttaatact tctatcaatg attttataat gataaaatat gatacaccta
      301 aaataaacat taatattcca gaaatagtgc cttgtagagt tgcttctcca ctaaaacaga
      361 ggtacataaa acttttaaat cctcccacaa caattactaa ccctaaaaga aaaaataaaa
      421 tttgtaatat accacttagt aaatttttta taagtttatt aggcatcata ccaactcctt
      481 taaaatattt tatatatagt ataatacatt atattatatc gtttttcaat aaaaatatta
      541 taattaacaa tatattttta ttgttaatta tgcaaataaa aaagagtata ataaaagcct
      601 ttaaaataca tttttttctt attttaaagg ctttttatat tctacatttt tattttctat
      661 attatatcct ttgataatcg ttacatctaa aatagaactt attaccctaa atataagtat
      721 ctttataact agaatagtcc attacaacag catctccaga ttaatccact gatacctctt
      781 tattatcaat atctaaaatt gaaattttac aattcccacc caatttttct tgaactattt
      841 tagctaattc atttaaagca ttttcaaatt ctttaaatct actttcatct actaattcaa
      901 tgcataaaaa ggcattgtta gtatcttcag taagcattgt tctcactgct tcacgccaat
      961 tccaacatct acaaattgct ccaccatcat ctttataaat tatttctcct tcatatggaa
     1021 atgaattttc atctgtccct agtgggataa actcctcatt accaattgct ctagttagcc
     1081 taatatctcc tataaattta tctatatcct cccctccaca aggtaatgca tattttaatg
     1141 agataaaatt gtagatatcc actaaagggt taatagttcc tatattattt ccattatgaa
     1201 ctctttttaa taatgcttca atagaagacc ttgctccttt tttcgtttta aattttttaa
     1261 atgcctctct ccatacttta atcacttggt tgttgctaaa ttctgagtcc tccaagtatt
     1321 ttaatgcttc ttcttctccc ttcaaaatta ttttcttata gtcttcatct tttatagagt
     1381 tatctatatc attacaaata ataactccaa tttttgcact aggaaataga ttccaaaatg
     1441 catcctcaat aacaaatttt ttcattagaa aacctcctca aatttaaaat atgtaaaaat
     1501 aaaaaagcct atagaaatat tattaaatat ctcccaggct tttatccttc cgtgtacaca
     1561 acaattgcgc gttttctctt ggacctgacc attataaaat gcggaaccct agaaaactaa
     1621 attataagtt ttttaattta caaaatacaa ttaaatttta gcataatata gcatttaaaa
     1681 caatattcta ttatctagtt tgtacattaa aacaatttgc cattagatta taattatggt
     1741 ggcaaatggg tggcaaatca ccttttttat tacatttttg tttgtttaaa ctattgaaaa
     1801 tactatattc ttacaatttt aatcaatttc tgatttaaat ggagtgtcat cagcttttgc
     1861 tattctatac tccttatcag ttatataacc aagctgatgc atcttataca gtacaatcac
     1921 ttgtctcttc ttagcctctc catggtcaat ataccttgca ggattatttg tcataccagc
     1981 taacatagca cactcagcta aatttaattc actaactttt ttaccaaagt agacttcagc
     2041 tccctttgca gctccatatg catttttacc tagatacata ttatttaaat atgcctcaag
     2101 aatttcatcc ttagtcataa tcttattcat ctgcttagca ttatacatat cccttatttt
     2161 ccgcttcata gttttatcat tactagtaag caggtttttt gaaacttgca tatctatagt
     2221 acttgcacct tgtgttgtat ctgtaaacac attgtttaat actgacctaa ctagagaaat
     2281 aacatctact cctttatgct tataaaatct ctcatcttct atggaaacta ttgcatgttt
     2341 taaatcattt ggcatttgtt ttatacttac aacctcacta cttatatatt tagacctaat
     2401 taacttttta gtaacttctg gtgacccttt tatagctgaa actgtaaata ttgcacctgc
     2461 cactgaaaat ataattagtg caactaacat aatagataat aatgccctaa ctattctaat
     2521 ccttttttgt ttttttcttt ttttactttt taattttttc tctctttgtt tatactcgac
     2581 tcttcttctt tctctttgat tgtaatctgt atttaaaggt cttacattat ctcttgtaaa
     2641 actatctcta tctcttttta taagattctg tgaatctact ttttcactca ttcttttact
     2701 ggaacttgta ttaggacttt tatttttttt atttgccgaa gtataagaat gtttagattt
     2761 tttattacta tctttgttgt tactaatttt tttacgtctt attttattat tatcattatc
     2821 attgtaataa cccattttag tctcccttct tcatataagg tacattatac attgttctac
     2881 aaaataaatc aaagaacaaa attgtaagct taagggtcaa ttttataaag gatattttca
     2941 ctaattttct ctatatttat aattaatatt catcttttat tttcaaaaat agttttgcag
     3001 ttgctaatgt atcactgtca gctctatgag catttatatt ctctatgttg taataagcac
     3061 aagctgtttc aagtttcttg ttttttcctt tataactttt acattttttt aataattcta
     3121 ctgtacatat ataattgttt attggttcta aatctaacat cctaagatag taatttaaaa
     3181 attttaaatc aaatttcgca ttgtgtgcaa ttatagttct atcacctatg tattttctaa
     3241 atctaggtaa aactaattct agtggttctt cattttctac catttcatta cttatatgtg
     3301 tcaattctgt tataaatgaa gaaattatcc ccttaggttt tatcaacctt gaatagtttt
     3361 ttacaatttg ttcatttttt atttctgtta ttcctatttc tattatttca caacctttat
     3421 atggatctaa tcctgtagtt tctaaatcca caactacata ttctttcata tctcttctcc
     3481 ttattattaa atggatatat ttatattcta tatttatgta atagactaat caatacttaa
     3541 ttttatatga tataggaaga ttttacaaat tacaacatag atatatccta tattccatca
     3601 taaataaaga tttttttatt agaataatta aacttttaat ttattgttat ttaatctatt
     3661 ttgataaaca ttatttattt tattattatt taaatcttta aaatctaaac ttttacattg
     3721 ttcatcattt tcccatattc ctgcttgttt atttccatca tttttatata atacaccctt
     3781 accatttttc ttattatcta aaaaatatcc cttataaatg tcattattgg gatatgttaa
     3841 ttttcccaaa ccatttttta atccgtattt aaattcacct gtatatattg aaccatctct
     3901 ataagtatat tttcctattc catgcatctt gtcatctttc cataatcctt catacttttc
     3961 cttacttgaa aaaatatata ttccaaatcc atttttcttt ccatcaacaa actcaccttt
     4021 atataaatca ccattatcgt atttaaatac tttttcttcc tttaatttct tattgttact
     4081 cttattttta ttattattgt tatcctttat atgtatttct tctcttctgg cttcaataaa
     4141 atctctatca tacctaaatc cgtttcttga tttaattttt tgattaatct tcttcttatt
     4201 aataaatact ctagctagtt gagtaaatat aaatacagat gataagcata taaatacaca
     4261 tagtattgtt gtaaaataac tagttatatt ttccataaaa aatcaccctt taaaaaattt
     4321 atttaaaata cctaatataa tatatatttt tattaaatgt tatttatact tttatagtac
     4381 tatattgaat aaaattaact ttgaaattat ttacattaaa ataaatcaaa tacaaatgcc
     4441 ataaattaag tgtttctagt tagcataaaa atacttataa agtatgctca aatatttcaa
     4501 ataaatactt ttattcaaat aaatctctac ttatataagt gcttatataa ataaaaagat
     4561 gaactatagt aaatatagtt catctttttt ataatagtat ttatttaaaa ttaatattta
     4621 tttagttagt agcatctact tagatatcag ataagaaagc acagtcattt acttataata
     4681 agttcttttc atatattttt tattataatt attcttttac aagaactttt ataatttctg
     4741 aaatgtacat agtatgatag tctttctctg gataccattt tttatcattt tctttagcat
     4801 caaagttttc tggtggcata ttaccatgat atagcttttt acatactata atcatattag
     4861 cttcttcaaa agctaccgta tcatccacaa aatatggtgt aagtcctgct ttttctattt
     4921 tatttatatc tctaccagaa atagtaccac aaatatttaa agcctctcta tacgtttcat
     4981 caaagaaggc aactgtaaat gtatcattac tatctacaaa ttcttttgta tatctctgtg
     5041 gtcttatgta agttgtaaca acatttttac cccaatagac tccaacacca ccccagctag
     5101 cagtcattgt gttaaacttt tctgaatcac cagctgttat aagtaaccat tctttgccaa
     5161 tcttagtaaa tggattaaac tgtagttcct caatttttac ttctctaaaa cccatgactt
     5221 catcctaccc ttcatttttt aattatataa attaattgtt aaaaatttga atatatgtac
     5281 tcttataata ctgacttttg attgtcctac ataattcatg taaattataa tacaacaaat
     5341 caattacttt cgcaataagt agttttcata ttttataagt gtaaaatata gataaaatat
     5401 attgattata aagtatataa tatattttta aacctaaagc aatatacaat aaaaaaatgt
     5461 agaaagtaag tatctctcta cattttttat tatatattat ttttatatta atttaatttt
     5521 atcttgattc agttttataa aaaattgtta taattgtcaa gttctactct attatttgta
     5581 atttcttata tcttgttaac aacatttaag ttattacatt tatttatata tattttttca
     5641 ttatctaagt cttcatcaaa catttttatt atatttttta tctcatactt gtactcatct
     5701 tcattcattc tataaagcat taaatcttct aatacatctt tcatgtcgag gtacacatct
     5761 tctaataaat aaaaatagcc ttcctctata tctttcaatt tatctatagt atccattttt
     5821 ttatttatat ctttcataat ttctaaattt tgtatattta catctatgat tgttaataag
     5881 tacattctca cagtttttat tatttcaaca gaaaaatcta caatattata atttccttct
     5941 tttttatata atgactcctt aaatttgtta aaacaattat ctaaattaga atttatatcg
     6001 ttaaatggtg gtcttattat gtcacgtatt attaaaagat tttgtatatc tacattattt
     6061 gtgattgcct catccaaaat aaataaatta tatgcatcat ttaatgggtc atgggcatct
     6121 ccactaaact ctaagttaac aaattcaagt aaatttttca atgatatata gttcatacat
     6181 ctcataccac aatttatata tttttctttt atatctacaa ataaatctct aatattattt
     6241 acaaaccttg gatggttatt ttttttagca cttctcatat ctgtgttatt gaaacatgtc
     6301 aaatccaaat taccaaaagt atatattcct ttaatatcag aaaatatgcc aagccatttt
     6361 ttaaattttc tcatgacctc ttcataactt ggtgcactct tcaattcttc agaagatata
     6421 tgtgttaatt cttgtatatg aggatataac tcctcattac ttactggtct tattaaagat
     6481 ttaaatttat caatattttt tgttttttca tcatacataa ctgctccgat tgcaattata
     6541 tcagaattaa atactgctct ttttgattta gaatttggca tattcatttc aaaatctata
     6601 tatactcttt tcaatataaa caccctttcc aattgtttat agtgattctt tttaactatt
     6661 tttattcaaa attttctaag aaagaatgtt tctccccatc tttttgccaa ccaagtactg
     6721 aatcaagatt tacattttct gctgccttaa atatagattt ttctacattt ttaaaatttg
     6781 attctaagct ctttattaag tcttttatct catatctttt attacctgtt tttttagcag
     6841 caaccataca tgcacaattt aatggccata taccactatt ttgaataaat cttataatat
     6901 gttcttctct tatataataa agtggtctta taatttttat accttcaaaa ttagtagaat
     6961 taagtttagg aagcattgtt ttaaaatttc cagcacacaa taagttaagc attgttgtct
     7021 ctattacatc atcataatga tgacctaatg caagtttgtt acaccctaat tcttctgcct
     7081 ttgaatataa tgctcctctc ctcattcttg cacacatata gcatggataa tcttttgcta
     7141 tttcatctgc tatctcaaat attcttgaat caaataaatg tattggtata tttagatact
     7201 cacaattatc tataagtaat tgtcttatat ttgcatggta tccagggtcc atagcaataa
     7261 attcaacatc aaaattaact tgtccatgtt tttttaactc ttgaaacatt ttagccataa
     7321 gaatactgtc ttttccacca gaaatcgcta cagctatctt atccccttct tcaaccaatt
     7381 tataatctct tattgacttc ataaacttag accataaatt ttttctatat ttctttatta
     7441 tacttttttc tatatctctt actggaagtc tttcctcaaa aggaactata atttcacagc
     7501 cttttcctga tagttcactc atctaatcac ctcgtatatt tatgtatatt tatgttaata
     7561 atattttaca aaaaagtcta ccttcatcca caaaatacta taatttttac tttaatttta
     7621 ctaaataatt tatagtttac aataacacat aaacttataa taacataaat aacctctttc
     7681 ttttattatc ttttttattt ttctcattta atattttatt attagtaacg atttttaaat
     7741 gattgaatat tatacactag atagagttac ctatctattt acaacaagga ggttttcatc
     7801 ttggaaaata agactagaga tatcaaaaaa aattgtggtc catctttggc tagaccatac
     7861 attaataatc aaattttcac agaaatgtac tgcctttctg atgcattaaa atttggaact
     7921 atatttccag aactaaactt actagagtct gaaatgtaca acaaagaatt atatgctaag
     7981 ccaaaaaaat atagaggagg tagaagataa tgaaagactc atctagagct gaacttatga
     8041 gaaaagttca ggaaacatct tttgcatgtg ttgatatgaa cttatattta gacaatcacc
     8101 ctgatgataa aaatgctatc aacacttata attcattatg taatcaattt gcgcaagcta
     8161 gatatgctta cgaaaataaa tacggtcctt taactaactt tgggtatgct ccaagtagat
     8221 gcccttggca atgggctgac caaccttggc cttgggacag agaatttaat tattagacat
     8281 caagtattgg aggattgaaa attcatgtgg atatatcaga aaactataca acacccagtt
     8341 aatataaaaa cttgtgaccc tagaatggct aaatttctta taactcaatt tggtgggcca
     8401 aatggagaac ttgctgcatc tttaagatat ttaagccaaa gatatacaat gcctactggt
     8461 aatatgcgtg cacttttaac agatattggt acagaagagc tagctcacgt tgagcttata
     8521 tgtactatgg tttatcaatt aacttctgat gcaagcccag aagagttaaa agctgcaggt
     8581 cttggttcaa actatgctca aaatggatat ggaatttatc caacagattc aaatggtgtt
     8641 ccatttgatg taagacctat agcagttatg tcaaatcccg taaccgactt acatgaggat
     8701 atggcagctg aacaaaaagc acttgcaact tattatcaac ttataaacct aacagatgac
     8761 gttgatgtta tagatgtatt aaaattctta ggtcaaagag aaataattca ctatcaaaga
     8821 tttggtgaag ctttaatgga tgcttacgag ttagaagaat ctcaaaaaat gttctaaaat
     8881 ttttatttgt ataactatta ttatttgata atttataatt tactgttgtg ttaaaattta
     8941 taaaaaatat aaatataatt tattttctat aaacagagaa aataaataaa aagcaggtat
     9001 caatcaaata gtaacatgta ttatactata cattactatc aatattgtac ctgctttcat
     9061 attataagca ataaagaaaa atctatttat taggttttat aataaaacaa ttataaaact
     9121 cttcttgcat ttacatatct acttgaatag tagctactat ttatattaga tattgttact
     9181 ttttttgatg aacctgaaga tgcatgtatc atatctccat ttcctatgta tattccaaca
     9241 tgacttacag aaccattatt tgaaccttgt gtatcaaaga atactaaatc tccacttcta
     9301 aggtctcctc tatttacata tgttccatac ttactttggt ctctagatac tcttggtata
     9361 ctaactcctg cagatttttt cataacgtac tgtgtgaatc cagaacagtc aaaactattt
     9421 ggaccttctg ctccccatac atatggtttt ccaagtagtg tctttgcaaa acttattaca
     9481 ctatcagctc cttgacttgg agaattagtc gaataagatg gaattgaagt atcaacagaa
     9541 cctttttcag caaggtattg acttgacaca tatccagttt gattgccata acttactttt
     9601 gtccatcctc cactttcaga aatacttcca acctcagtac cataacttaa tgtagctatt
     9661 ttagaatgac ttgtacctgg tcctgttcta acgttcaatg actttgctgt tacaaccttt
     9721 gtacccttta caaggtcatt tgaactgttt tcaacatttt catttgtaga gtcatttaca
     9781 ctagttccta aatatttact tgaaacataa cctactctac cattagaact tatttttgac
     9841 catccatttg actcagatat gtatccgact tcagtcccat atcctagtgt tgatatttta
     9901 gaactacttg tacttggtcc tgttctaaaa tttaatcctt ttgctgttac aacttttgtc
     9961 gacttaactg aagtatcact actttcattg ctacttccaa ggctatttat tgctacataa
    10021 tttccatgca cataacctgt gtttccatta tatttaactt ttacccaaga accttctttt
    10081 cctacgtact caactttatc acctttgtaa agtttcccca ttgattcatg gtttgtacct
    10141 ggtccttttc taaaatttac cgcattccct gttattgttc tgctttctat actcacactc
    10201 tctacttcag actcattagc atatgctaaa ttccccatag taggcattat tatcgcagtt
    10261 gccattatac tggctgctat tctcttttga ttaaaattca taactttcca ctcatctcct
    10321 cttttctact atctttattt cacttcaaaa tataaaagaa ataattgaca ctattaaaat
    10381 atcaattatt tcttttatat taatattatt acaaatttgt aactatttgt ctatagtaat
    10441 atttgcttac cataaattac catgtataaa ttttaatctt caatagcctg taatttagta
    10501 atatagccat cttttacctt ttttaaaaat ggctatattt tttaactttt ttgtaacttt
    10561 ttaaaatcca gttgagccaa acccaccttt tcctctttcg gagtcactta attcttttac
    10621 ttcttctatg ttaatatcat agataggttt tactatcatt tgagcaattt tcatatgctt
    10681 ttcaacctta aaatcatttt tcccatgatt tattaatata attttaagct ctcctctgta
    10741 tccttcgtca atagttcctg gtgtatttaa tacagtaaca gaatgcttta aggcaagccc
    10801 acttctaggt cttacctgcg cttctgtcat agtaggaagc tctatacata tcccagtttt
    10861 gataagttta gtctctccag gaggtattac aacctcttct atagaataca aatccatacc
    10921 agcatcacct ttgtgagcaa aattaggtat tatagcatca tcatttaatt tttttacttt
    10981 taaattgtac atcttatatc tcctttcaac tttctataaa tattataact taaatttcga
    11041 cctcttgaag ttttttattt ttttgtggta atataattat tagtgtttta aaggaggatt
    11101 ttttatgaaa cttgtacttg ctgagaaacc atctgtggca aaaactatag catcattttt
    11161 aggtgcaaag accagacaag atggttactt tgaaggaaat gattatataa ttacttatgc
    11221 agttggacac ttagtatcat tatatgatat gaaggattat gataaagata aatactctgg
    11281 ctcttggaaa atgaacaatt tcccatttgt tcctgaggat aagtttaaat ttaagattga
    11341 tagttctaaa actaaacaat tcaacactgt aaaaaagctt ttgaatagaa aagatgtaga
    11401 atatgtaata aatgccactg acaatgaccg agaaggagaa cttatagcat ttttaatatt
    11461 tttactggca aaaaataaaa aaccagtaaa aagaatcttg gtaaatgaat ggacaccaga
    11521 agatattaca agaggtataa aaaacctaaa agatgaatat gaaatgagaa atcttcaagc
    11581 tgctggatat actaggctta ttactgactg gttgattgga ataaatttca cttctgttgc
    11641 tacattgaaa tatggaaatg ggaaactatt aaacataggt agggtaattc ttccaacagt
    11701 taaattagtc tatgatagag atatggaaat actaaacttt gttcctaaaa cttattatga
    11761 aatagaagga cattttaaag ctgaagctgg tgaatataaa ggtaaatatg ttaagggtaa
    11821 agaaagtaaa tttgatacac ttgaagatgc aaataaaatt atagcttcaa ttacatctga
    11881 aactggtaaa atactagata aaaaagttac tatgtcaaag gaatatgcac caaaactttt
    11941 tagtttgaca tcacttcaag gatatataac ttctaagtat tccaatttta catctgataa
    12001 ggttttaaat gtctgtcaat ctctctatga gggtaatggt aagggtggat acataactta
    12061 ccctagaact gattccattt tcttagaaga aagtttggct tcaaaagctt ctcaaacttt
    12121 agataaatta aaggttggtc ttgaatatga aaataaaatt aaattttcta aaactaaaag
    12181 agtttttgat tcttctaaag tagactccca tagtgctata attccaactt atataattcc
    12241 aaaaagttta tctaaggatg aacaattagt ttatgatgcc ataaaagata gatttgtagc
    12301 aaactttatg cctcctgctg aatatgagaa tacagaaatt aagactgaag tagataattg
    12361 tactttctta acaaaaggta aagtgcttaa atcaaaagga taccttgagg tatataataa
    12421 ggaagaaaaa aacgatttac ttccacttgt aaataaagat gatgttgtag atgtgttaga
    12481 aataaaacca ttaactaagc aaactactcc acctaaacca tatactgagg acaccctttt
    12541 aaaggctatg aaaaattgtg gtaaaaatgt gcctgaagaa gatacaacag tgctatctgg
    12601 atattcgatt ggaacatcag ccacacgtgc tgatgtgtta aagaagataa gtcaagtggg
    12661 ctatgtaaag aaaaaaggga aatcttattc tataacagac cttgggaaaa atttagttga
    12721 aatttttcct gttaaagact tatttgatgt agattatact ggaaaacttg aaaaaagttt
    12781 aagcgatatt caaaaaggaa agtatacaag aaaagaatat cttactaata tcattagttt
    12841 tatatatcaa aatgtcaata ttataaaaca agatgcttca aaaaatataa acacagaagc
    12901 atatactttc gattcaaaaa ctaaaaaatt tactaaaaca aaagaaaaaa aacagaaaaa
    12961 agatgtaact aatactgaaa aagaagttgc tatatcaaaa gattctaata acaaaaaaga
    13021 agttaaaaat ggtgatgaaa atcaatctct tggcaaatgc cccatttgtc aaggtgatgt
    13081 actagaattt gacaaaggct ttgcttgtaa aaatcataaa gaatgtaaat ttgttatttg
    13141 gaaaaatgat aagtatttaa gtctttataa aaagaaagtt aacaaaacaa tggtcaaaaa
    13201 tattttaaaa aaaggtgaaa ctaaagtaaa atctttaact gctaaaaacg gtaataaatt
    13261 tgatgctaca ttaaaatatg ataaaaatcc agataccggc tatttcaatt ggaaaataga
    13321 gtttgacaac taaatattca taagaaaaga aatagcagta gtataataat ctatattata
    13381 ttactgctat ttcttttctt atgaagtttt aactttttta atagtgactt ttacaatcac
    13441 actctaacct aatttcttta ctatttctga taactttaat gtatgttcat ctaataaagt
    13501 tagttcctct tcagaaagat tagataattt ttttactaga gattctttga gaatagcttt
    13561 tactgcatca aataatttaa atgctttatc tgttaattca accctaagaa tccttctatc
    13621 ttttgggtca ggaaatttat tgactaaacc atgttccact aatctgtcaa taatcggagt
    13681 catatttgat tttaaaattc ctaaattaga agctatttga gaaattgatg atgaatttga
    13741 tgtcataaga taaagaatta ccttagcatg tgatggagcc attggacatt cttcaaaata
    13801 agctttcatt atatcagagt tattttgaaa cttttttaac aaatcgcttt ctttaaatat
    13861 attattttca agcacaaata aaaaatctaa aaaagagtcc gctattttaa gtatattgtt
    13921 gttatccata tttgtaacat cccttcaata gataatataa taaaacattg ttgtttaata
    13981 tagtataaat tataaactat taataaattt aaaacaagaa aaagttattg actcataata
    14041 attataaatt tataatcaaa ttagagaatt ataaattgcc aataaaggga agttttttaa
    14101 ttagtctaaa actaaactta aattataaga cactttaaat tctacaacaa gctagtgtgc
    14161 aatatataat aggaggagat atgaaaagat atttaatagt tttcttaatg tgtagttcac
    14221 tgtttttagt tggatgtgga aaaacaaaag aatctgaacc tgagaaacca atagcagttt
    14281 ctgttcaaaa agcagtaggt ggagaaattg aaaacacaaa ttctttctca ggaactacaa
    14341 aagttaaaga tgaaacagct gttactgcac agactgttgg cacagttcaa gaagtgtatg
    14401 taaaacttgg gcaaaatgta agaaaaggag atgaattatt gagtataagc agtccagagc
    14461 tagaaaatag tgtaaagcaa agtaaagcct cactagattt agcaaaagca agttattcaa
    14521 gtgccactgg tggttcatta gaggcacaag taaatcaggc aaaaacagct cttgacaatg
    14581 caaagattca atatgaggag tctcaaagaa attatgacaa taataagatt ctttatgaac
    14641 aggaagtcat tagtttagac caatttaaaa aaatagagtt ttctttagag caaacaaaac
    14701 aacagttaga ttctgctcaa agagcatacg atacagcaac ttcaaaaagt atacctcaag
    14761 ctaaagcact tgctaaaaaa cagcttgacc aagctcaagt atcatataat ttagcaatga
    14821 gtaatttaga caaattaaca ttaacatctc cagtagatgg tacaataaca gctaaaaact
    14881 ttgattcaaa ggaaatgata acacaatcac aaccagcttt tattatttct aatcctaata
    14941 tactagaagt tgatttaaat gtagcagaat cagatatagg taaatttaag aaagatggaa
    15001 atgtagatgt tataattgaa gaccaaagga ttttaggaaa aatagattat gtaccttcag
    15061 tagttgaccc tcagacatct ttatatcctg ttaaagtact tgtaaataat gcaaacaaca
    15121 aatttaaggc aggtatgtct gcacaagtaa acttaagtat tgaaaaagaa aatggtgctg
    15181 ttacagtgcc taaaaaagca atatttgaag aaaatggtaa aaaatatgta tacatagcaa
    15241 caaaagacaa tatagcaaag aaacacttgg ttcaaactgg aattgtaaca gaggataaaa
    15301 tagaaattaa aagtggtgta agtgataaag atacagtgat tattggagga ataagtctta
    15361 tttctgacgg aactaagata tttcctgtag aaaaggagaa gtagactatg aatttaacac
    15421 agacttccgt taaacgtcct ctcacaatca taatggtctt tttagtatta atagtttttg
    15481 gtggtattgg atataaaaaa atgtcaataa atctaatgcc tgatatagaa ataccagttg
    15541 taatggtcat gactacttgg acaggtgctg gtcctcaaga cgttgatgaa caagttagtc
    15601 aaaaagttga tgaaagttta tctgccgttt caaatgtaaa atctactata tcaagttcac
    15661 aagaaagtgt ttctatggta gttgctcagt ttgaatttgg aactaatttg gatgaaataa
    15721 tgaatgatgt taggtctaaa gttgatgcat tacagaccag cctcccagat gatgcagcaa
    15781 aaccaacagt tttaaagctg gatatgaatg cacaggctat aggtcaactt gtcatatctg
    15841 gaggaaatga aaatagttca caagccttaa gaaaatacgc tgaagatgta attcagccta
    15901 aaatagaatc catagatggt gttacttcag cagaccttaa aggtggagaa aaagcacagg
    15961 ttaatgtaat agctgaccca gctgttcttt caaattatgg agttagttta tccactataa
    16021 aaggtgtctt atcctctagt aataaaacat tcccttatgg ttcaattact caaggtgagg
    16081 ataaaatagt tttaagagca attgataaat tagaatccct agatgatata aaacagattc
    16141 aaattcctgt aaaaggagga aatacagtta gattagataa tctttgtagt gtagattatg
    16201 gatatgcaga caaagatagt atttatagat acaatggaaa agacagtttg gtaatagata
    16261 tttctaaaca acaagatgcc aacactgtaa aagttatgca gagtgcatac aaatacgtag
    16321 atgaattgaa taaggaaaat cctcaattta aaattaaaat tgctaatgat acaagttcat
    16381 acataagtga atcaataaac agtgtaatga gcaacttgct tataagtgct gttatcgcat
    16441 ttattgtaat atttgctttc ctaaaaagtg taagagcttc actagttgtt gcagttgcaa
    16501 taccaatttc tataataggt gctatagcaa ttctctactt tactggtgaa agtttaaatc
    16561 ttattactgc aagttcattg gtaatttcag ttggtatggt tgttgataat gctacagttg
    16621 taattgaaaa tatatttaaa tataggaaaa gtggcagatt gagtcttgat gattgtgcta
    16681 ttgagggaac aagaactgta acaaatgcaa tacttgcatc aacattgacc acgatagcaa
    16741 tattcttacc tatcatgttt acagaaggta tagctaaaat aacttttggg gcattaggta
    16801 aaacactaat agcttcacta gcattctcct ttataggtgc aattacactc gttcctagtg
    16861 tatttgcaaa attaagcaga ggaaaaaata gtcaaaagat gcaagaaaaa gatagtccta
    16921 tatttgataa ggtaagtgaa gcatataaaa aattattaag tgtatgttta agacatcaac
    16981 gcttagttgt aatatcaagt ttacttattt ttgtagcttc cttatttgga atgggattta
    17041 taggtatgga ctttatgcct gcagcagaca agggtgaatt atctatttct atagaacttc
    17101 caaaaggtct tagcctagaa tccaatgatt actatgtatc tatgactgag aaaaaagtct
    17161 cagatattcc agaaattaaa actttaataa caactttgac atcagattca aataatagta
    17221 aagcaagtat agcaattgaa ttagaatctc aaaaaaatag aaaaagaact actgatgaaa
    17281 tagaaaaaga gataattgat agagtggcta ctgtaccaga ttgtaaaata aatgtaagtc
    17341 aaaacagtag tgttatgggt ggagctggag gagcagactt cacattagaa atgaaaggtc
    17401 ctgatttaga tgtattaaaa atactgggaa aagaagctga aaaagaattt agctctataa
    17461 aaggatttag aaatgttgaa acttctctta cagatacaac acaagaagct caatttacta
    17521 tagataaaca taaggctcaa gaatatggtg taaatactgc tgaaattgca ggcatgttac
    17581 gtacagcaat aaatggtgac tccgtaacca ctgctactat tgatgattac aaagtggatg
    17641 taaacctaaa atttaaagca gatactattg atagcttaga agatataaag caactaaaag
    17701 taaactctgc aactggaaaa gaagttcctc ttggtgcttt ttcagatata aaaatggcag
    17761 atggtctaca atctatatct aggactgatg gagatttttc tgtaaatata actgctacac
    17821 ttgacaattt agatgctggt actgctacaa aactagcact agaaaaaacg aataaaatgg
    17881 taattcctaa agattactct ataggtgttg gtggtgaaac agaaatgatg aatgaatcta
    17941 tggcaagtct tggattttca atggtcatag ctatagcatt agtttatatg gtaatggtag
    18001 ctcagtttga atcatttagt aaaccattta taatcatgtt ctgtgttcca tttgcattcg
    18061 ttggagttgt gctttcgctt ctagttagtg gtcaaagttt aagtactgtc ggtatgctag
    18121 gtataatctt acttatcggt attgttgtta ataatggtat agttcttatt gactacatag
    18181 agcaacttag aaaaacaaat cgtaacaatg atttaattgg tttggtttca attggttgtg
    18241 cagcaagatt aagaccagtc ttaatgacta cagcaacaac aattttaggt atgttgccaa
    18301 cagcactatc tcttggtgaa ggtggagaaa ctatgcaacc actagccata gttataatgg
    18361 gtggtctaac tgtatctaca cttataactt tggtgcttat acctactata tacttaatat
    18421 ttgataaatg ggaaaataag ttttcagaca aaatgcctaa aatatttggt aagataacag
    18481 ataaaatagg aagtataaaa cgtaaaataa taaagaaacc aaaattgaaa gctaaagttt
    18541 aaatagaatg gagggtgatt gtattgaata aaaaaatgag caaaatagtt gcaattggct
    18601 gtggtgttgg tataataact gccaatttaa tcccagttta cgcagataat aatacttctt
    18661 ctataaacaa cgttgataat tctattttaa gtgactcaaa taaaagaaca tctgaaaagg
    18721 aaattttgac tgctagagaa gctgttagtg ctgcacttaa aaatagtgaa aaattaaaaa
    18781 tgaaatcaga agaaataaaa atgttaaaag aaaagttaga agtacaagat gagtttgata
    18841 gttttactgg aagtgacaac tcgtttccat acgaccaaat tggacttctt aaaaatcaaa
    18901 gtgaacaggc taaaggcttt atggaggacc aaatagcaaa tgatattact aataaattta
    18961 acgatttagt ttcaagagaa aatgagctag ataagataaa aaacaatctt gaaataaaaa
    19021 ctaaagaaat taaagatatg aaattaaaaa aagatttagg gttagtaaca tctttagaaa
    19081 ctgaatcagc tgaacttgaa cttcaaacac tacaaaatac tcaaaaagct aaacttcaag
    19141 aactaaaaaa caatcaagac tattttaagt tattgactaa tatagattta aataattatc
    19201 aattggataa agaatataga tttgaatctt ttagagtagg tggctcagtt gattcatata
    19261 tggaaggtaa ggttaatgaa tatttaaaat acgaccaatt gattttagaa cgtacagaag
    19321 aaagctttaa tgataaagat gagaacaaag cagatttacc agatagacct aatcttacaa
    19381 gaccagttgc tcctacaaaa ccagagcaag gtaacctaac tgatgatgaa tacaaggttc
    19441 ttatggataa gtatgaaaaa gactacaaga gatatgaaaa agaagttgaa gcatacaatc
    19501 ttgaaagaca gacttatgct gctggcctta ctacttatgc aaattatctt caacaaaaat
    19561 ttaataccga aaatggtcta gtaacattag aagacagtaa aaaagctctt aaaaaagggt
    19621 taattgatag ctatgctcaa ttacttgctt tagaagatac aattcagatt actaaaaaac
    19681 aacttgattt atcagaaaaa caattaaaaa atactaaatt aagatatgat ttaggattaa
    19741 ttactctaac tgattataaa aaacaagtgg taagtaatca agatgctaaa aatagctatg
    19801 acactcttat tgtgaattat aactcactaa aaaatggtat agaaaaacct tggatactta
    19861 atactggaaa ataattctgg taaatagaaa gattacttta acatacgttt gattaaaaaa
    19921 tagttatgca cactcgtggc tttagtcatg gaagattcag aaggcaaata atagaaatag
    19981 attagtaaaa atatatttac ttataaaact gtatactttt atagaggcta tctcaaaatt
    20041 ggatatgtcc ctgtcaagta gacagactta aaaaaggcta tctttatgcg gaatgagttc
    20101 gatattcaat cgaactcatt cc
//
LOCUS       DAGQWR010000079        19882 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.079, whole genome shotgun sequence.
ACCESSION   DAGQWR010000079 DAGQWR010000000
VERSION     DAGQWR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.079"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            294..1004
                     /gene="rgaR"
                     /locus_tag="MIW95_002380"
     CDS             294..1004
                     /gene="rgaR"
                     /locus_tag="MIW95_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBY3151702.1"
                     /translation="MFRVVICDDEKIQRSILKEFTKKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
     gene            2050..3081
                     /locus_tag="MIW95_002381"
     CDS             2050..3081
                     /locus_tag="MIW95_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBY3151703.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            3214..4512
                     /locus_tag="MIW95_002382"
     CDS             3214..4512
                     /locus_tag="MIW95_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBY3151704.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(4626..4871)
                     /locus_tag="MIW95_002383"
     CDS             complement(4626..4871)
                     /locus_tag="MIW95_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBY3151705.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            5019..5885
                     /locus_tag="MIW95_002384"
     CDS             5019..5885
                     /locus_tag="MIW95_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBY3151706.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(5889..6425)
                     /locus_tag="MIW95_002385"
     CDS             complement(5889..6425)
                     /locus_tag="MIW95_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBY3151707.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(6615..6818)
                     /locus_tag="MIW95_002386"
     CDS             complement(6615..6818)
                     /locus_tag="MIW95_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBY3151708.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            7109..7873
                     /locus_tag="MIW95_002387"
     CDS             7109..7873
                     /locus_tag="MIW95_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY3151709.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKYDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            8262..9575
                     /gene="prdC"
                     /locus_tag="MIW95_002388"
     CDS             8262..9575
                     /gene="prdC"
                     /locus_tag="MIW95_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBY3151710.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            9744..9906
                     /locus_tag="MIW95_002389"
                     /pseudo
     CDS             9744..9906
                     /locus_tag="MIW95_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            10376..12136
                     /gene="prdR"
                     /locus_tag="MIW95_002390"
     CDS             10376..12136
                     /gene="prdR"
                     /locus_tag="MIW95_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBY3151711.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            12565..14445
                     /gene="prdA"
                     /locus_tag="MIW95_002391"
     CDS             12565..14445
                     /gene="prdA"
                     /locus_tag="MIW95_002391"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBY3151712.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            14526..14810
                     /locus_tag="MIW95_002392"
     CDS             14526..14810
                     /locus_tag="MIW95_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151713.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            14848..15573
                     /gene="prdB"
                     /locus_tag="MIW95_002393"
     CDS             14848..15573
                     /gene="prdB"
                     /locus_tag="MIW95_002393"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:15298..15300,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBY3151714.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            15653..16411
                     /gene="prdD"
                     /locus_tag="MIW95_002394"
     CDS             15653..16411
                     /gene="prdD"
                     /locus_tag="MIW95_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBY3151715.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            16426..16893
                     /locus_tag="MIW95_002395"
     CDS             16426..16893
                     /locus_tag="MIW95_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBY3151716.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            16911..17393
                     /locus_tag="MIW95_002396"
     CDS             16911..17393
                     /locus_tag="MIW95_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBY3151717.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPAVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            17413..18420
                     /locus_tag="MIW95_002397"
     CDS             17413..18420
                     /locus_tag="MIW95_002397"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBY3151718.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            18540..19421
                     /locus_tag="MIW95_002398"
     CDS             18540..19421
                     /locus_tag="MIW95_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBY3151719.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         7906 a         2170 c         3568 g         6238 t
ORIGIN      
        1 tatgaatttt atgtcttaaa atttataagc aataaaaatt tgatatatta tgatgtttaa
       61 tatagggtaa taaatacata ttatatttat tttactatat ttttttacat aatatatcta
      121 gaaaagtata ggaatttatc gtaatttaag tttaaatatt aaagagaatt tctataagta
      181 gtttaatcga caacttaaat ttataaagaa atttaaattt gaaagctagt gagtacagtt
      241 tggaaattga aataaagtaa actaaatgat attttagatt tggaggtgat attatgttca
      301 gggtagttat atgtgatgac gaaaaaatac aaagaagcat actaaaagag tttacaaaaa
      361 aacttttgtc tgaaagatgt ctaaattatc aaatactgga attttcctgt ggagaagatt
      421 taatatccaa atacccagaa aaaattgata ttatattttt agatatccaa atgaaagata
      481 tcaatggaat tgaggtggct aaaaaaataa gaaagtttga tgaaaaagta gaaattatat
      541 ttacaacagc tttttcagag tatgcccccc aaggttatga ggttcgtgct tatagatatt
      601 tagtaaagcc tatagagtat agtaactttg caatgggtgt aaatctttgt attgataacc
      661 ttcaaagaaa gaaggagcgt tatatagttt taaatagtaa aaaaggattt aatagaatac
      721 tgatagattc tattttatat gttgaaactg taaagagaga tttagttgta catacaatag
      781 gtagagatta taaagctaat atgagcatga aacaagcaga aaaattactg tgtgaaaatg
      841 gcttttttag atgtcataca agttttattg ttaatttaag aagaatagaa gatcttaggg
      901 ataatatgat tactattaat gaacaattta tacctataag taagtataga cttaaagatt
      961 taaaagtagc attaacaagc ttgttatgtg atataatgta ttaattttaa aaaggtaatt
     1021 gaaaatatcg taaaaaagat ttaatgtcat cacagggcta ttgattaaaa ctgtcttaat
     1081 atataaaatg ttatttatat taagacagtt ttattatgca aaatatcagt ttatagactt
     1141 ttaatattgt ttaatatgta tatgtagagt ttccattaca gtagcatgat tattaattag
     1201 tagcctgatt attaatatac tattattgat ttttatatgc attatatgta tttttagaat
     1261 atgattacct ttttaaaata atgactattt ataaaactct atagatattt gtttcattta
     1321 gctatgcata ctttaattaa ttatattaat atttaatgga gtataatata aatattaaag
     1381 cgacatctta tagtttattg aacctatagt aatgaattaa taaatggttt aattctatga
     1441 aaagatttcc ttttatgcaa tttatttatt gaaatatgat ataatatata aaggtgtaag
     1501 tattagttgt taaaataata tttatattag tttttatgca tacttggtat aagtttttca
     1561 tatattggtt aaaattgatg ttgatttatt atttttagta ttttttcacc ttttgattca
     1621 tactttgtag aatggtgtat tttataaaac tgattataag acaagttcat tcaaattagc
     1681 atttttatat catataaata attgttcccc ctaaataaga tttagatgta tagtattaaa
     1741 atcaaaaagt ataaaataaa attgatgctt aatcatagat aaatattaat aaaatataca
     1801 aggggattgt ctaataaaaa gataatctcc ttttttacaa ataatcctaa attgtgatat
     1861 aaatctaaaa attttatctt aacatttaac catttaatga taataattga tataataaat
     1921 ataaattaaa tatatggtgt acttatttta tgtttttagc aagaaatata gcatttaaaa
     1981 gataaattgt gcaacttaaa tcaaagttta agatgaacta ttaacataga tatattaaaa
     2041 acttttgtaa tggagaatat atgtgatatg aataataaag ttaaggttgc agtattagat
     2101 acaggaatag acaaagaaca tgattactta aaagataatt tagttggagg aattgctttt
     2161 gaatgtatgc atgattatat ctttatatca gataaatttg atgatgaaga tggtcatgga
     2221 actgcttgtg catcaataat aaagaaagag tatgaagatg tagagttatt tgtaataaaa
     2281 attttgagaa aagatggtat tacaaatata aaagttcttg aagaagcatt aagatacctt
     2341 ttagacacta acataagatt aattaattta agtttatcag tcataggagt agagagtgtt
     2401 aagggcttat ttgaagtttg ttacgaattg tttagaaaag gaaaaataat agtatgttca
     2461 ctcgctaatg attttgattt aagttatcca gctatgttta acaatgttat aggtgtaaga
     2521 ggatttactt tagatggaga tgattcattt tggtacaaca aaaaatatga tgttcaatgt
     2581 gttatggata gcaattcata tataagttgt gatataaata attcctatag attaccacca
     2641 aaatgtaata gttatttatc agcaaagctt actggtaaaa tagctaagat attatcagaa
     2701 aaaccaggca taacaatatc tgatttaaat gataagcttg aatcattagc aactagaaat
     2761 cattgggata gttgtgattt tgatgagtgt agtagaattg taaactttaa attggatttg
     2821 tatgacaaag aaaatgctct tcttatgaaa gttgctgacg ttgttaggga ttgtttagat
     2881 attgaagata ataatgaaaa actctttaag ggtagccttt ttaataagga gataggtttg
     2941 gtttatgata attgttttaa tctgttgaaa aagctagagc atagatttga cataaagttt
     3001 aattatatgg acatctcaaa atatgactta gtttcaatat atacactgac tgaattagtg
     3061 gaaagatata aaaacttata aataaagatt tgtagctaaa tttaatgtag aaggtattaa
     3121 attatatgta ttctttgtcg gtagattaaa agtaaaaaat atactataat tagatagcat
     3181 aagagaaaat gccaatacag ggggagttag ataatgaaat atgaagaagc attagagtac
     3241 atatcacaaa ctaataaatt cggtataaga ttaggattag aaaatatcgg aaagttatta
     3301 gaattattag gaaatccaca agaaactttg aatataatac atgtagctgg aactaatgga
     3361 aaaggttctg tgtgttcttt tgtttctaat atactaagag agtgtggata taaagttggt
     3421 ttatatacat ctccatattt agaaactttt actgagcgta taagagtaaa tggacaaaat
     3481 ataccacagg atgatgttgc tagaataatt gagttaataa aagaaaaaat agagattatg
     3541 gtaaaagaag gctatgcata tccaacagaa tttgaagtgg ttactgcaat ggcattttat
     3601 tactattcag aacaaaaggt agactttgtg gcattagaag ttggtttagg tggcagatat
     3661 gatgctacaa atataataac aaaatcactt gtaagtgtga tagtatctat aagtttggac
     3721 catacaggta tacttggaga tacaatagaa aaaatagcgt acgaaaaggc tggaataata
     3781 aaggaaaatg gagtagtact agtatatgac cagactgatg aagcgaagga tgttataaag
     3841 tcagtttgta aagagaaaaa agctaaatat atagaagttg attttgatga tataaacata
     3901 aaaaaatcag atataaattc tcaaatatac gattgtactg taatgaaaga aacttataga
     3961 gaccttgaaa taaaattgat aggtgaacat caaataaaca attctatctt ggcaattagt
     4021 gtaataaaat atctaaaaga cataaataaa ttagcaaata taaatgaaga atctataaga
     4081 aaaggactta taaatactaa atggccagga agaatagaaa agataaaaga aaatccaatt
     4141 tttataatag atggtgcaca taatgaagat ggagcaaaat ctttggcaaa agcacttgat
     4201 aagaatttta aaggtagaaa attaacctta ttaataggta tgttagaaga taaagatatt
     4261 gatagtgttt tagagatatt gttgccacat tttaataagg taataacaac aacaccaaat
     4321 aacccaaggg ccataaattc agatatatta agagaaaaag tattaaaata tgtagatgat
     4381 gtaacatcaa agcatgagat agaggatgca gtaaattata cattagaaac atcaagtgag
     4441 gatgatatta taataagtgc aggttcatta tatatgattg gtacagtgag aactttagta
     4501 aagaaattat aattttataa aataaatata caaataaaga tataaaaaag agttgtctaa
     4561 aaaatggaca actcttttta tataaagtaa gtttttaata attttactga tgtttatata
     4621 agtaattata tagattgacg tatagcctta gataattggt ctgggcaaga agtacctttt
     4681 ccaccacagt caataccgtt taatttatca gcaacttcaa gagcattttg gccgattatt
     4741 aagcttctaa gtcctgataa attaccatta catcctccta taaactcagc atcaacaatc
     4801 acattatttt catcaacttc aaaaatcatt tctctacaac atacaccact tggattaaat
     4861 gttactttca ttaaaaagac ctccattaaa tattatttct ttttttaaca ataataataa
     4921 taccaaatat atgcgtaata atgcaactat aaagcataat acaagctaat gaaataatat
     4981 attattgtag tatatgtatt gtagagagga ggggttatat gaaaatctat atttctaact
     5041 acttatcaaa gagtattagc atagttgatt attctacact tgaactagaa aaagaaatag
     5101 tattagaaga taatatatat ccacaccatt tttgcataga aaaagaaaaa aatttaatat
     5161 acatacctag ttcaagtaat gggatacttt atgtcttaga tttaagtaat gataaaataa
     5221 ttgatactgt ctctatagga ggaagtctta gtcaagttgt acttagtgat aatgaattgt
     5281 ttgtggcaaa tgaagattct aatagtatat atgtactaga taaaaacaca ttaaatccaa
     5341 taggaattat tggagttgat aatatgccac atggatttga ctttgataga gaaagtaaaa
     5401 aattatatgt accgtgtata aactcgatag tatgtataga tacaattaat aaaaatatac
     5461 aaaagaaaat aaatatggac tttagagctt ggcatataga gcttgataaa caaaagaaag
     5521 aaatgtatat atcaacactt gatggaaagc ttataataat agatgaagta agcatggata
     5581 taaaaaaggt attatatgaa tttttattac cagtagaaat aagatttaat tatagtggca
     5641 aaaaagttta tgttgctgat ttgggttata ataatgtaag aattttagat tatacaacga
     5701 gtagatacat tggaaatatt gaaataaatg ggattcctca aggcttagaa atatctaaag
     5761 atgaaaagct actatttgta tcagatacac aagaaaattc agtaaaagtt tatgaaactg
     5821 caaacaataa attaataaaa gaaatcaagg taggaaaaga gcctacaact ataatttgtt
     5881 tgtaggcttt aatgtatgtc tcttataagt aaatcgataa cttcggcata catataagat
     5941 gcattatttt tccagttatt gcaaataagt tttgcttgtt tctctgatga aacatttaga
     6001 tttaaatcta ttaagttaga ctgattttct atgactctaa ggttgacaat aaattcatta
     6061 tcactttgtt ttacaaaact agactttact tgagtatctg ctagtagatt ttctttgtta
     6121 gaatctatat acttgtctat tttttcagta acactagtag gtattcgatt aaagaagtat
     6181 tctagacttt ctacgcctct ttgagtaaga gcataatatt ctctgtcaga gtctttatat
     6241 gtagtcaaaa acttagattc cataagctga gataaaagct gttgtaatga aaaataattc
     6301 atcatctcag tctctaatac tacttgagtt atttgagaat tagttaaatc cattttaatt
     6361 ttttctaata tatataaaat taaaagtttg tgataggcta attcttcaga cgagttttca
     6421 aacataaata tcatcctccg tcaaactatt tctatatata atatattaaa ctaatttgta
     6481 caaaattaaa agaacaattt gtattgtatt attaatcttt aataattcat ataaaacaat
     6541 tttataaata aatatagaga aacagggctg ctaattagca gccctgttga tacccaggtg
     6601 tcaaaattta ctatttattt tccagccatt tgtctttcag ccatttcaac taatttctta
     6661 gtcatatatc caccaacata tccattttct ctagctgtta agttaccttt atctatgttt
     6721 tcatagttag ataaacctat ttcgttagct atttctaatt tcatttggtt taaagcagct
     6781 tttgcttctg gaactactgt tctgtttgaa tttgacatag taaatttcct cctcaaataa
     6841 ttagtttatg atgtctaaca acacttttat ttatgtgatg ttaatactaa tttctccaaa
     6901 ataaaaataa tatatacaga aaaataaagg taaaaatgta aaaaatataa aaaaattaaa
     6961 taaatgagta ttttagagag tttaattagg tcaatatagg ttttattttt ttagttattg
     7021 tcttataaat taaatattac agtacttttt tgagctgaaa aaaaataaat tttaaatata
     7081 gaagaaatta tttcaaaggg ggaatagtat gaaaagagaa gttaaaagga ctaaagcttt
     7141 gatatatagt gttttatttt tttcattatt attagtgtta atatttgttt ttgtttatgg
     7201 aataaaatac atatgttcta aatacgacaa ttcaaaaatg cctatatata aagttgatac
     7261 aaatgagaaa aaaatagcct tgagttttga tgtagcatgg ggaactgata atatggatga
     7321 tattttaaaa attttagata aacataatgt aaaagctacc ttctttttag ttggtagttg
     7381 ggtggatgat aatgaagaaa ttgtaaaagc tatagatgaa aaaggacatg aaattggaaa
     7441 tcattcaaac actcatgcca atttgaagga aatatcaaaa gaagacataa caaaagagat
     7501 agaaactaca tcagaaaaaa tatataatat aacaggaaaa aagactaatt tatttagacc
     7561 tccttttggg gacgtaaaca ataaagctat ggacatttgt agtgatttag gttataaagt
     7621 aattaagtgg gatgtagatt ctatagattg gaaggagctt ggaccaaatc atgtaattga
     7681 gaaagtaata aaagaatctc aacctggttc aatagtttta ttccatgcta atataaatga
     7741 tgtagataac tatttggata ctataataag tcgactaaaa aaagatggat acagtctagt
     7801 caaaatatct gatttgttgt ataaagataa ttatgtagtt gattccaatg gagtgcaaaa
     7861 aactaagaat taataagtaa taaatgtata ttttaagtaa agttgtgata aattatatag
     7921 tatatgttag aaaagtgtcg ataaaaagac ggtgtctatt ttttgacact ttttatacat
     7981 gtatgcaata tcaataatta tagattaaat tgattaaaaa agtgtctaat aattgacttt
     8041 tttttgatgg atttaaaaat ttagaagaat taatgaatgc tgtaagacag tatttccaaa
     8101 gggtataagt ttgtttaaaa gttggcatag aaattgcttt gttaataagc gatatgttct
     8161 atagaaataa acaatatgtt aagaccttat aatagaaaaa tagcattaac attatttgaa
     8221 cagcttataa aaaaattaat atacttaagg gggcgtacta gttgaacaaa ctatattcta
     8281 ttttattaaa acaacacgtt ggtggtccag ataagccagt agtaagtgtt ggagatgtag
     8341 taaaaaaagg aactctaatc gcagaaccaa ctggattagg ggcaaatatt tatgccagtg
     8401 ttagtggaaa aataagtgaa ataaatgacc aagcgatagt aattgaagct gatgaagtac
     8461 aagaagatac ttttgaacca ttaaaaggag aaggtatact tgacttaatt aaagaagctg
     8521 gagtagtagg aatgggtgga gcaggattcc ctactcatgt aaaattaaac atagatttaa
     8581 atggtggaac aatattagca aatgcagctg aatgtgaacc tttattagct cataatataa
     8641 aagaaataga agaaagacca gaaatagttt atcaaggtat aaaatatgct atggaagtta
     8701 ctaatgctgg aaaaggtatg ctagcaataa aaagcaagca tccaaaagca atagaagctt
     8761 tcaaaaaagt aataaagcca ggagacaaca tagaagttgc agaactagtt gatatgtacc
     8821 caatgggaga agagagagca atagtaagag acgttctagg aaaattactt gagccaactc
     8881 aattaccttc agaggctaat gcagtagtaa taaacgtaga aacactaact agaatagtag
     8941 aggctgttga gcaaaaaaga ccagttatat ctaaaaatat aactgtagtt ggtcaattaa
     9001 acagtggaaa agagtctata gtatttgaag atgtgccaat tggaacaact gtcggagaat
     9061 taattgaaag agctggtgga ataaaaggtg aatatggtga aataatccta ggaggacctt
     9121 tcactggtaa agctactact ttagatgctc caataactaa aactagtggt ggtataatag
     9181 ttactatgcc atttgttaat gaaaagagaa aaatgggtct tttagtctgt gcatgtggac
     9241 ctaatgaaga gagaatgaga gatatagcaa ctaaaatggg agttactgat atagtatcag
     9301 ttcaaaaatg taaacaagct caagaaatta aaggtgcatt aaaatgtgaa aatccaggac
     9361 actgtccagg acaagctcaa aaatgtattg aatttaagaa agctggagca gaagttatat
     9421 taataggaaa ctgtactgac tgtagtaaca cagtaatggg ttctgcacca aaattaaagt
     9481 taggtgtata tcatattact gaccatgtaa tgagaacagt taatcaccca ttaataagaa
     9541 gaataaaagg tgacgtaaga ttaaaaataa aataatagat aaatagaagt tagacatctt
     9601 tagaagtaaa acttaaaaat tacttctaag gatgtttttt aatgtatttt gagggcaata
     9661 tatagcttta aaagaaatca aaaaagtgcc aaatgtaaaa ctaattaaac catatatatt
     9721 taagaaggga ttgaattgga gttgtgaatt atgttagtag aaaccattct aagtgtttgt
     9781 tattggtaca tttgatattt gtatgtaaat acagaaaaaa ctttttgctc agatggatac
     9841 tttgctttca gtgtaggaaa tgtttctaaa gaaatgattg aaaaatacat acaaagtaag
     9901 ggataggttt acatccacca acctaaaggt atagtggttt tacgccttaa tttataaaaa
     9961 gaaattaatt tatattatta ttaagtttaa aataaaaagt ctcaaattga tttataagtc
    10021 attttgggac ttttttatgt aaatataata tgcaaatata gtttgtttaa aaatatctat
    10081 tttaattata ttcttaaatt tatgattgga tacaggcatt cattaattgt ttgatgaata
    10141 gaaaaatgtt ttgaaggtga aaatagtatt gaaaaaataa tatactaagg gtataattca
    10201 tttgatttca tttgtataaa tttaattata aattttaaag tgattatata aatttaaatg
    10261 ctaattactt aataaatttt aataataatt atttaataaa taattattat aatatgctag
    10321 aataaatata tataaataag ttttgatgag gtactactat aaaggggggg actttatgtt
    10381 ttcgatacca gaagtaaaaa aagtagaaga ggtaatggat acaaaattta caactataga
    10441 cgaagacaca agaattgaag atgcaataaa agaaatgata aaaagcaaca caaaaactct
    10501 aatggtaata gattcatctg accaattaaa aggcattata tccatgacgg atatacataa
    10561 tctttatgag atgcataaaa agtatgaagg tcaaccagta aaacttataa tgaagaagga
    10621 tgtaatatat gtcaacgaag gtttaacctt agatgaatgt agagatataa tgatattaaa
    10681 aaatataggg attcttcctg ttcttagaga taataaaatt attggagttc taaaacaaga
    10741 gcacattagg gactatctat atatgcattt agaagattat ggattgactt tgaagtatat
    10801 tataggtcaa attaaagaag gaatttgtgc tataaataac gaaggtgttg ttatcttgtg
    10861 gaataagttt atggaggaaa ggtatgatat aaagtctgaa gatatagttg gtcgaccgat
    10921 gaatgaattt ttggaaaata ctatttcaga aaaagtacta aatagtaaag ttggaatgag
    10981 tgacttgtat tttacagata aaaaagaaaa tatgtatgca ttagttcatg caaatcctat
    11041 attttataaa gaagaattta tcggtgttgt atgtacagaa gttgatgtta cagaagctaa
    11101 gatattagct cttgaacttg aaaaagtaaa tgatacatta aaatatctaa agaacgaagt
    11161 caaaaattta tctaaaggta gttttgataa gattttaggt aaaagttata aattggaaaa
    11221 atcgaaagcg attgcaaaac aagtggctag aacaaatagt agcatattta tttggggtga
    11281 aagtggtacg ggtaaagaag tatttgcaag agctattcat gattatagtg agagaaaagg
    11341 tcaatttata cctgttaact gtagtgccat accaaatgag ttatttgaaa gtgagttttt
    11401 tgggtatgaa tcaggtgcat ttacaggagc aagtaaaaag ggtcgaatag gtatatttga
    11461 acttgcaaaa gatggaactg tatttttaga tgaaattgcc gatttacctc ttagtatgca
    11521 agcaaagttg cttagagtat tacaggaaaa agaaataaga agagttggtg gagataccac
    11581 aataaaaata aatcctagaa taatatcagc taccaataaa gatttgagta aaatggtaaa
    11641 ggcagaaaaa tttagagaag atttatacta tagattaaat gttgttgaaa taaagattcc
    11701 tccacttaga gagagaaaag aagacatagg attattggtt catagctttt tagaggagat
    11761 atgtaaacaa aataataagc ctgttcttac aattagcaaa gatgtaatag atatatttca
    11821 gaattataga tggaaaggta atattagaga attaaaaaat acgattgaaa atattgttgt
    11881 attgtcacaa aattctaaaa tagatgtaga tgatgtacca agttatatga tggattctac
    11941 aaataatagt actgaagaag aagagtatcc cttagattta acgaaggcaa ctcaaaaaat
    12001 tgaaataaag aatataacca aggcacttaa aatgtctaat ggaaataaag ctaaagcagc
    12061 taagatatta aatataccca gaacaacttt gtattataag atagaccaat acaaaataga
    12121 tgtgtctaaa atatgacata tgactaaatt ttgacaaaat gataaacttt aatttaaaac
    12181 ctagtaaagc caacacttgt ataagtgttg tgtatataga tgtgttaaaa aaaatgaaaa
    12241 agtgttgatt aattgacact ttttttgttt gagaaaatgt ttacaaatac aaaaaataac
    12301 atattttaaa taaatattta tataatattt ggctaaaaat agcgatagat gaagaatcta
    12361 ttatagaaaa aatctttttg ttaataagtt ggcataggaa ttgcttataa tataaagtga
    12421 ctttgagtga gaaaaaaata atattaatca taacaaatta tgtaggtttt aatttactta
    12481 atttctcagt taaggggtta cctgttgagg aattgagatt taaaataata aataagaaaa
    12541 taatgtaagg aggtatttta tactatgtca ataactttag aaacagctca agcccatgca
    12601 aatgacccag cagtttgttg ttgtagattt gaagcgggaa caattatagc gccagaaaac
    12661 ttagaagatc cagcaatatt tgcagactta gaggattctg gattattaac aataccagaa
    12721 aatggattaa ctataggtca agtactagga gctaagttaa aagaaacttt agatgcactt
    12781 tctccaatga ctacagataa cgtagaagga tacaaagcag gagaggctaa agaagaagta
    12841 gtagaagaaa cagtagaaga agcagctcca gtatcagaag cagcagtagt tccagtaagc
    12901 acaggagttg caggtgaaac agttaaaata cacataggtg aaggtaagaa cataagctta
    12961 gagatacctt tatcagtagc tggtcaagca ggagttgctg ctccagtagc aaacgttgct
    13021 gctccagtgg caagtgcagc agcagaagta gctccaaaag ttgaagaaaa gaaactttta
    13081 agaagcttaa ctaaaaaaca ctttaaaata gataaagttg aatttgctga tgaaactaaa
    13141 atagaaggaa ctactttata catcagaaac gcagaagaaa tatgtaaaga agctaatgaa
    13201 actcaagagt tagttgtaga tatgaagtta gaaataataa ctcctgataa atatgaaact
    13261 tacagtgaag ctgtattaga tatacaacca atcgctacta aagaagaagg cgaattaggt
    13321 tcaggtataa ctagagttat agatggagct gtaatggtat taactggtac agatgaagat
    13381 ggagttcaaa taggtgaatt tggttcttca gaaggtgagt taaatactac tataatgtgg
    13441 ggtagaccag gtgctgctga caaaggtgaa atattcatca aaggtcaagt aacaataaaa
    13501 gcaggaacta acatggaaag accaggacct ttagctgctc accgtgcatt tgactatgta
    13561 actcaagaaa taagagaagc attaaagaaa gttgacaact ctttagtagt tgatgaagaa
    13621 gtaatagagc aatacagaag agaaggtaaa aagaaagttg ttgttataaa agaaataatg
    13681 ggacaaggtg caatgcatga taacctaata ttaccagttg agccagttgg tacattagga
    13741 gctcaaccaa acgttgactt aggaaacatg ccagttgtat tatctccact tgaagtatta
    13801 gatggtggta tccatgcatt aacttgtata ggacctgcat caaaagaaat gtcaagacat
    13861 tactggagag agccattagt aataagagct atggaagacg aagaaataga tttagtaggt
    13921 gttgtatttg ttggttctcc acaagtaaat gctgagaaat tctatgtatc taagagatta
    13981 ggtatgttag ttgaagctat ggaagttgat ggagctgtag taactactga aggtttcgga
    14041 aacaaccata tagatttcgc atctcacata gagcaaatag gtatgagagg tataccagta
    14101 gttggtgtaa gtttctcagc tgttcaaggt gctctagttg ttggtaataa atacatgact
    14161 cacatggtag acaacaataa gtctaagcaa ggtatagaga atgaaatatt atctaacaac
    14221 actttagctc cagaagatgc tgttagaata atggctatgc ttaaaaatgc tatagaaggt
    14281 gtagaagtta aagctcctga aagaaaatgg aatccaaatg ttaaattaaa taacatagaa
    14341 gctatagaaa aagttacagg agaaaaaata gtattagaag agaatgagca atctctacca
    14401 atgagtaaga agagaagaga aatatacgaa aaagacgaaa actaaaattt agatactaac
    14461 taagatttag acaataacat aaatttagat ccaagtttaa aacagtatag agaggtagaa
    14521 ttataatgga taggttaaaa tatatctcat cagagacttt ttatgaaggt attatagttg
    14581 acatcaaagg tggtggagtg actatagacc taaaaggtag acttggacaa tttaaaatac
    14641 ctaatagaat gcttataact gactacgaac ttaagatagg tcaagaagtt ggattcatgc
    14701 taagctatcc tgaggtatta agtccagagc ctaatcaaga gtatgtagaa aatattagga
    14761 gagaaaaaga aaaacaagca gaaatgcaat caaaacaaga agaaaaataa taaataatcg
    14821 tttgaaaata gaagggagag gaaatatatg agccttacaa cagtacaagg acttcaatct
    14881 gaaatattcg ttccaataac acctcctcca gtttggactc ctgtaactaa agaattaaaa
    14941 gatatgacta tagctttagc tacagcagct ggtgttcatt taaaatctga caagagattt
    15001 aatctagcag gagatactac atttagagct atacctaata cagctacagt tgatgaaatg
    15061 atggtatcac atggtggata tgacaatgga gatgtaaaca aagatataaa ctgtatgttc
    15121 cctatagata gattacatga attagctgca gaaggattta tcaaaggagt agctcctatg
    15181 cactatgcat tcatgggtgg tggaggaaac caacatgtct tcactgaaga aactggtcct
    15241 gctatcgctg ctaaacttaa ggaagaggga gtagacggtg tagttatgac agctggctga
    15301 ggtacttgcc atagaactgc cgtgatcgtg cagagagcaa tagaggaagc tggaatacct
    15361 acaataataa tagcagctct tcctccagta gttagacaaa acggaactcc tagagcagtt
    15421 gctccattag ttccaatggg tgctaatgct ggtgaaccac ataatataga aatgcaaaca
    15481 catatattaa gagatacatt agagcaatta gttgcaatac catctgctgg taagatagtt
    15541 ccattaccat acgaatataa agctcacgtt taaaatgtaa tttatatttg aaaatataga
    15601 acccttctcc tacacggtta ggagaggggt tttaagaaca aaggtggata ttatggaaga
    15661 aaaaataatg agaagacttg ttataaaacc gtttcatatg aatgaagtta atttcggttc
    15721 aaaaacttct ataaagaaag atgtacttac aatagattta tcaagtatag atgaaataaa
    15781 agaaagagaa gagttaataa cagacataaa agtagacatt ataaaacctg gagattatga
    15841 tagagaaatt aatactatta tggacattat accaatatca acaaaagttt taggcagact
    15901 gggagagggt atcacacata ctttaacggg tgtatatgta atgttaactg gtgcagatga
    15961 agatggaaat caaatgcatg aatttggttc ttcagaagga attttgaaag accaaatggt
    16021 atttgggaga tatggaactc catcagttga agattatata atccatgtag atgtaacttt
    16081 aaagggaggt ttaccttttg aaagaacact tccacttgca gcattcagag catgtgatga
    16141 ttttatacaa gaaataagag cgtctttgaa aatggaagac ggaagaaatg ctactcaagt
    16201 tcgtgaatat tttgacaaaa ttcgaccaaa tgcaaaaaag gttgtcatag taaaacaaat
    16261 agcaggtcaa ggtgctatgt atgacaatca gttattctca aaggaaccta gtggatttga
    16321 aggaggaaca tcaattatag atatgggaaa tgtacctatg attatatctc ctaatgaata
    16381 cagagatggt gcccttagag ctatgactta agagaggtgg atattatggg aataggacct
    16441 tcaacaaaag aaacatctct tcatcacttt agagacccac ttttagacat agttgaaagt
    16501 gataaggatg tagatttact tggagtaata gtagtaggaa ctccagatgg aaatgaaaat
    16561 aaaacctttg tgggacaaag aacggccgct tggctggaag ccatgagagt agatggagct
    16621 atagtttcat cagatggttg gggaaactca catgttgact acgctaacac atttgaggaa
    16681 ataggaaaaa gagatatacc tgtagttgga gttacattta atgggactca agctaagttt
    16741 gttgtaagca atcaatatat ggataccata gttgatatga acaaatcaaa ggaaggtatt
    16801 gagacagaag tagttggaga aaacaacaca aatgaaatag atgctaaaaa agctttagca
    16861 tttttaaaac ttaaaatgag aaaacatgga taaaaaattt aagtaaggga atgagttttg
    16921 tgggaatttc tacatcaatg aaaaacacat cacttcatat atttagagac ccgttactag
    16981 atttgataga taatgattct gacatagact tgttaggagt ggttgtagtt gggacatcag
    17041 agcacaatga atggaaaaca tttttagcaa ctagacttgg aagatggatt gaatcattgc
    17101 gacctgatgg agtgattata actttagata tggcaggaaa ccagcacatt gattttacaa
    17161 atgctatagc agagtttgta aaatcagata tacctgcagt aggtcttact attatgggag
    17221 cagatggatt agtaattacc aatccatatt tggataaagc taccataatt gattataaaa
    17281 agacaacagg ctacatagaa acagaagtag ttggagacaa tcacatggat gaagttgatg
    17341 ttaaaaaagc attggctttc ctaaaattaa aaatgcgtaa agacgcaaaa taaacctaag
    17401 ggggaatttg atatgaaatt tagcagaagt atacaagcta tagactctca tacagcaggt
    17461 gaagcaacta gaatagttgt aggtggaata cctaacataa aaggaaattc tatgcctgag
    17521 aaaaaagaat atttagagga aaatctagat tatttaagaa ctgctataat gttagagcca
    17581 agaggacata atgatatgtt tggttctgta atgactcaac cttgttgccc agatgctgac
    17641 tttggaataa tcttcatgga tggtggtgga taccttaaca tgtgtggcca tggtacaata
    17701 ggagctatga cagcagctat agaaacaggt gtagttccag cagtagagcc tgtaactcat
    17761 gtagttatgg aagctccagc aggaataata agaggagacg ttacagttgt agatggaaaa
    17821 gctaaagaag tatcattctt aaacgtacca gctttcttat ataaagaagg tgttgaagtt
    17881 gacttaccag gtgttgggac tgttaaattt gacatatcat ttggtggaag cttctttgct
    17941 ataatacatg caagtcaatt aggtttaaaa atcgaacctc aaaatgctgg taaattaact
    18001 gaactagcta tgaaacttag agatataata aatgagaaaa tagaaataca acatccaact
    18061 ttagctcata taaaaactgt agacttagtt gaaatatatg atgagccaac tcatccagaa
    18121 gctacttaca aaaatgtagt tatatttggt caaggtcaag ttgacagatc tccatgtgga
    18181 actggaacaa gtgctaaatt agctacttta catgctaaag gtgaattaaa agtaggagaa
    18241 aaattcgtat atgaaagtat attaggaact ttattcaaag gtgaaatagt tgaagaaact
    18301 aaagttgcag atttcaatgc tgtagtacct aaaataactg gttctgctta tataactgga
    18361 tttaatcatt ttgtaataga tgaagaagac ccacttaaac atggatttat tcttaaataa
    18421 gtaataaata aattaggaaa ttaaaataga atagtagcca cttgtcggtt gctattctat
    18481 agcttttagg ctataagatt cagaatatat gaaaaaataa aaaaatagga ggatggatta
    18541 tggtagcaat cttaaaggca ttatatgtag tattcgcagc aatctttgca gttgtgtttg
    18601 gtaaagacgt agctaaagca aacaaagagg ggaaactaga agaacaaagt actccaaaag
    18661 ttgctgtaac aggatttata acagacttct ttgatacatt aggaataggg tcatttgctc
    18721 caacaatagc aatgtcaaag gctttaaagt taaatatacc agataaaaaa atgcctggta
    18781 cattaaacgt agcacatact attccagttg ttacagaagc attcatattt acatcaatca
    18841 taccagttga tggtgttact ttagtatcta tggttgtggc agcagcagtt ggttcatata
    18901 taggtgcagg aataatagct aagatggacg agagaaaaat acaacttgtt atgggtgtaa
    18961 ctttagcatt aacagcaata ttaatgttat taggtcatcc ttggattaac gtattaccag
    19021 gtggaggaaa tgaattaggt cttactggtg ttaaattggt aataggtgta ataggtaact
    19081 ttatactagg agctttaatg acagctggta taggtctata tgctccaggt atggcaatgg
    19141 tttatttctt aggaatgtct gcaaaagttg cattcccaat agttatgggt tcttgtgcat
    19201 tattaatgcc agttgcaagt atgaaattta taaaagaaga tgcatatact aaaaaagcat
    19261 cagttataat agctatatgt ggtttaatag gagttttcgt agctgcatat atagtaaaaa
    19321 ctctaccaat ggatatatta aaagcattag taatagtagt tatagcttat acttctgcta
    19381 caatgataat ggctgcaaat aaaaacaaaa agactgctta gttaaagact acttagtgtt
    19441 tattaaatat tacagctaat aataaagatt ataaactgct ttaaaatatt gaattaatat
    19501 tttagggcag ttttttattt gctaaattaa tataaagcaa ttaacatgcc aattatgtaa
    19561 tatatatagt gatttagtag atttaatgta aaaaacgttg tattttcaaa gaataaagca
    19621 taatttaaag ctaaatattt aaattttata atcaataagt tcaataaatg ttcatatatt
    19681 aatacagatt aataaaaata taagctgtaa taagtctaat ataacttata attaagaaaa
    19741 ataagtttat aatgactcaa tattcatctg aattatttga ataaatttaa ttcgttgaag
    19801 ttgcttatat agagtgaata aatttaagtt atatatgact tattaattat ataataggat
    19861 ttttataatt aatacaaaac tg
//
LOCUS       DAGQWR010000080        19840 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.080, whole genome shotgun sequence.
ACCESSION   DAGQWR010000080 DAGQWR010000000
VERSION     DAGQWR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.080"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            300..821
                     /locus_tag="MIW95_002399"
     CDS             300..821
                     /locus_tag="MIW95_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3151720.1"
                     /translation="MDIAKLILNPARLRILQYIRLHGSARTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVVDNDKTK"
     gene            855..1631
                     /locus_tag="MIW95_002400"
     CDS             855..1631
                     /locus_tag="MIW95_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY3151721.1"
                     /translation="MKFHEFGDKNKPHIMLIHGGGNAWWNYLRQAEVLSNNYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKGYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1974..2927
                     /locus_tag="MIW95_002401"
     CDS             1974..2927
                     /locus_tag="MIW95_002401"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBY3151722.1"
                     /translation="MINVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2945..3964
                     /locus_tag="MIW95_002402"
     CDS             2945..3964
                     /locus_tag="MIW95_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBY3151723.1"
                     /translation="MFKKCIKVVTLTFILACILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3995..4246
                     /locus_tag="MIW95_002403"
     CDS             3995..4246
                     /locus_tag="MIW95_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151724.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4528..6333
                     /gene="lepA"
                     /locus_tag="MIW95_002404"
     CDS             4528..6333
                     /gene="lepA"
                     /locus_tag="MIW95_002404"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBY3151725.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6484..7821
                     /locus_tag="MIW95_002405"
     CDS             6484..7821
                     /locus_tag="MIW95_002405"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBY3151726.1"
                     /translation="MINIEIPKKVDYIIKELETNGYEAYVVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLISTEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILRRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            8054..9367
                     /locus_tag="MIW95_002406"
     CDS             8054..9367
                     /locus_tag="MIW95_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY3151727.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTIISLGGIFITLLGLPVYYYMKKR"
     gene            9539..10714
                     /locus_tag="MIW95_002407"
     CDS             9539..10714
                     /locus_tag="MIW95_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBY3151728.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMKLY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10817..11836
                     /gene="hrcA"
                     /locus_tag="MIW95_002408"
     CDS             10817..11836
                     /gene="hrcA"
                     /locus_tag="MIW95_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBY3151729.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11866..12486
                     /gene="grpE"
                     /locus_tag="MIW95_002409"
     CDS             11866..12486
                     /gene="grpE"
                     /locus_tag="MIW95_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBY3151730.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12578..14425
                     /gene="dnaK"
                     /locus_tag="MIW95_002410"
     CDS             12578..14425
                     /gene="dnaK"
                     /locus_tag="MIW95_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBY3151731.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLADEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14636..15790
                     /gene="dnaJ"
                     /locus_tag="MIW95_002411"
     CDS             14636..15790
                     /gene="dnaJ"
                     /locus_tag="MIW95_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBY3151732.1"
                     /translation="MSTKRDYYEVLGISKDAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16756..17691
                     /locus_tag="MIW95_002412"
     CDS             16756..17691
                     /locus_tag="MIW95_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBY3151733.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     AEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKDYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17727..19100
                     /locus_tag="MIW95_002413"
     CDS             17727..19100
                     /locus_tag="MIW95_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3151734.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEGNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19288..>19840)
                     /locus_tag="MIW95_002414"
     CDS             complement(19288..>19840)
                     /locus_tag="MIW95_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3151735.1"
                     /translation="IFYNLFEDINIIQHNGFFIVNSKYNLDINVTTQNIIETETYQST
                     YIFYLGELDSKADFYFRLQLCSDLLPHIIKDNAENKFLNLFDLIRYKTLDLINEDNIL
                     NKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTLVYRLEKINEILGFD
                     LKNFENAMIFYLSVKSYFLYKKI"
BASE COUNT         7870 a         2186 c         3565 g         6219 t
ORIGIN      
        1 atacaagaaa ttactcattt ttgtaaattt aatgaaaaat tataaaaata taaatttata
       61 tgcttaatta agataatttt tccaataatc aagataattt ttttcattag tatgtatgat
      121 agatgcatca aacaatataa ctaatatgtt agaaggttat atgtaattaa tctcagtgtt
      181 tatatagaat agcatttgta gcatttatta tagttgtaat tactacttat atcatagact
      241 tgaatagtct aaaaaaatag actataatat ttttatagac taaagaaagg gctgataaga
      301 tggacatagc aaaactaatt ttaaatcctg caaggcttcg tatcttacaa tatattcgac
      361 ttcatggcag tgctcgcaca tctgatattg taaagtattt aaatgatatt cctcgtgcaa
      421 cagtttatca ccatgtaaaa attttagaag agaacaatat gattgaagta attaaagaaa
      481 atcgtgtgcg tggtactatt gaaaaggttt atgcactaaa ggaatataca acatctatgg
      541 aaggagagac cttcgttgca ctttctacag cattttatgt aggtctgatg caggaaatga
      601 atgaatactt tagcagaaaa gagcaagacc ataaaaagga taatgtattt ttctcttcag
      661 cattactata tatgagtgat aatgaatatg aaaatcttct taaaagtata gctgatttat
      721 tgaaaccata tatagaacaa aagcctaaat caaatctaaa acttagaaaa ctttcaatta
      781 tatcttctcc acctgtggtt gataatgata aaacaaaata acatgaatct tatttttata
      841 aaaggaggtt gtatatgaaa tttcatgaat ttggagataa aaacaaacca catattatgt
      901 tgattcatgg gggagggaat gcgtggtgga actatttgcg acaagcagaa gttttgtcta
      961 acaactatca tgtgatactt cctacattgg atggtcatgg agaagaatat gaaacaacat
     1021 acatttctac agaggataca gcagataagt tgatatcata tatagatgaa aattgcagtg
     1081 gacatctttt cgctttgtgt ggggtatcac ttggtggtca aattgtcatg gaacttttat
     1141 ctcgaaaacc taatattact aaaaaagcaa ttattgatgg tagtatttgc tacccaagac
     1201 ctcttatggc aaggttttgt attgctggta ttcgattttt tggaggactt ttgtttagtg
     1261 agaaggcttg tcgctttcaa attgctatga tgccaaaatt gttacctgaa aatatgcaat
     1321 atccagatga aataaaagcg tattatatga cagatatgcc acatgtaaga aaagaaacat
     1381 tctataatat gtatcgtact tatatgatga actatacact taaagagaat gttaaaaaaa
     1441 ccacagcaca agtaatgtac tggtatggag aaaaagaaat gaagtgcgtt aaaaaatctg
     1501 caaaaatgtt tcagtcttat gtaccctctt gtgagattta tgaagctaag ggctataatc
     1561 atgggtattt atcgctttat cttccaaatg aatggttgga aattgcagaa ccatttttcc
     1621 aaaaggaata aaggtgttag gtgattattg tataattatt gctgtttttt tatatagtaa
     1681 ataaattagg tataaatata aaaaagttat tttagttaat atattagtga aagcttaata
     1741 ggaaaattat aaatagatta aaggtagcgc ctatataaat agttgctacc tttttttgct
     1801 tgtaaataaa tgatagataa gaaagtttac tgacaaatca ctgactaaac ccaattttct
     1861 tacattgaaa gccttaattt tacttgtttt aaataaattt acaatttctt gttataaaat
     1921 actaaatttt gttaacaatt ataattagca tttaatttat actggaggtt tatatgatta
     1981 atgtaagaac agatttagct ttagaagcta gtgaaatgtg tgaaaagtct caagaaggaa
     2041 gtagtattcc tggtgtaaaa atagaaacta aagaattaga aaattgcatt gtaactaaag
     2101 ttgaagtaat agacaggcaa ggcgcagaga taatgaataa agatatagga aagtatataa
     2161 ctctggaaag taatcttatg aagtttgatg atgatgagtc gagagaagaa atgataagtt
     2221 atttaaaaga ggagttagta gatatatttg gccaagacaa aaataaaaaa accctggtaa
     2281 ttggtcttgg aaatagaaat attacttctg atgcgttagg accaaagagt gtatcaaaaa
     2341 cacttgtaac tagacatctt tttaaaaatt ataataaaga ctatgatgat gatttcactg
     2401 aggtatcagc tttaagccca ggagttatgg gtgttactgg aatagaaact agtgaaatag
     2461 taaagtcatt ggttgaaaaa gtaaaaccag atagagtagt agcaatagac gcattggctt
     2521 caagaaaaat ggaaagagta aactctacta tacagatatc aacagcagga atatcacctg
     2581 gtggaggtgt tggcaatact agaaaatctc taactaaaga aactttaggc gtggatgtta
     2641 ttgcaatagg cgttccaacg gtggttgatg cagcaactct tacaatagat gtcttagata
     2701 tggcaataga caatttaata gctcaatcag aagaaacaga aagtttttat gaaatgttga
     2761 aaaaacttaa agaagaagaa aaatatcatc taataaaaga ttcgttagac ccatatgaca
     2821 agaacttaat agtaacacca aaggatatag atgatacaat cgaaaatcta tctataataa
     2881 taagtgaagg cttaaatagg tctttacatc caggtagatt ggtataaaat aaggaggagc
     2941 aattatgttt aaaaaatgca ttaaagtagt aaccttgact tttatattag catgcatttt
     3001 accaggaaaa tcattagctt tgaatcagga tgatttttta aagtttttag taaattcatc
     3061 ttatccagaa gctaaagtcg aaggaaatga tacagaaaat aaaaagaata ataaaaataa
     3121 agaaacaagc aaagaaaata aggaagaaag taaagaagaa aacactaaat ctaaagatgc
     3181 aagtaaagtt gataataaaa aagagagtga aaaagaatat ataaaacttt atgttggtaa
     3241 agaaaatgtt ccagatattg agtcaaaaaa ttcagacaca actgaaacta acacaacatc
     3301 tagttcagat tataaggatg atttaagagt aaccaaagaa aatccaagaa ttttgatata
     3361 tcatactcat ggatgtgaga cttattcaaa ttcacctgat ggaaattatc attcaagaga
     3421 taagaaaaat tcagtaatgg aagtaggaag tgcattaact agtgctttag atagcaaagg
     3481 atggggtgta gtccatacta ctaaatacca tgactatcca tcttataata actcttatgc
     3541 aagtagctta aaaacaatac aaagtatatt acctaaatat aactcagtgg atatagcgat
     3601 agacttacat agagatgcta gggatttaac taatccagcc actaaggaaa aagaccattt
     3661 aaaatataca actatgataa atggagagag agtatcaaag ttcttctttg ttgtaggagg
     3721 taaaaataca aatagaaaac aacttagagc tttagcagaa gatataactg cttttgctga
     3781 gaaaaagtat cctggtctag tatctccaat agtagaaaaa gactatgcta gattcaatca
     3841 gtttgcagtt aagaatcata tgttagtaga aatagggaat aacgcaacaa gtgtagaaga
     3901 atctaaggct actacaaagt atttagccga gattttagat gaatatttta aacaaaaaaa
     3961 ttagagcttt aagttataat taggagcatg ctaaatgagc cgattagaaa aaaatataga
     4021 aagtaaaagt aaactaagaa aatacaagta tattatgaga atcttatttt tgattactat
     4081 gattataggt acatcagttt gtgtatttat agtagacagt aatgcaaaaa gtatgctagg
     4141 aatgaaacaa tatgattttc caagtattaa atttgatata acaaaatttg attttgacat
     4201 accaaatttt gatatacatg aaatcataaa aaaatttaat aagtagtaat aagtgaataa
     4261 ggtttattga ttaaaataag ccttatttta gttatatatt tttaatgtat aaaataaaaa
     4321 ttcattaact gtattttata ggatttactt taagtttacg atattatata atatttagtg
     4381 ttagacttga taatccacat ataatgtgga taattttgtt tagtgccaat tagtttatat
     4441 tcaaaaattt taaatttggt atataaagag tttttagata atgtaaaata ataactataa
     4501 gaataatcaa gaggagggaa ttttaaggtg gacaataaac aaagtagaac tagaaatttt
     4561 agtataattg cacatataga ccatggaaag tctaccttag ctgatagatt aatacaacaa
     4621 actgggcttg ttagtgaaag agacatgaaa agtcaattac ttgacaatat ggaccttgaa
     4681 agagaaagag gaatcactat aaagcttcaa aacataagat taatgtataa agcgaaggat
     4741 ggaaatgaat attatttaaa tcttatagat acacctggac atgtagactt taactatgag
     4801 gtatcaagaa gtttagctgc atgtgaagga gcactattag tagttgatgc agctcaaggt
     4861 gttgaggcac aaactttagc caatgtttat cttgctatag accaagattt agaaatatta
     4921 ccaataataa ataaaataga tttaccaagt gcaagaccag aggaagtaaa aaatgaaata
     4981 gaagatttaa tagggcttga ttcaagtgaa gctcctctta tttcagctaa aactggttta
     5041 aacatagaag atgttttaga agatattgta aaaaatgttc ctccacctaa gggagataat
     5101 gaagctccat taaaggcatt aatattcgat tcttactatg atgcttacaa aggtgttgta
     5161 gcttatgtta gagtatttga aggaactgtt aaaaagggta tgacaataaa aatgatgaat
     5221 accaataaaa agtttgaggt aactgaagtt ggtgttatgg ctccaggtca aactgaactt
     5281 agtgaattat cagcaggtga tgttggttat attgctgcta gtataaaaga tataagaagt
     5341 tgtcgtgttg gtgatacaat aacagattca aataatccta cagaagaacc tttaccagga
     5401 tacaaaaaag ctactccaat ggtatattgt ggtatatatc caggagaagg agaaaagtat
     5461 gagaatgtta gagatgctct tgaaaagctt caagtaaatg atgctgcact tgaatatgaa
     5521 gctgagactt cagcagcact aggatttggt tttagatgtg gtttcttagg tcttttacat
     5581 atggagataa tgcaagaaag acttgagaga gaatttaacc ttgatataat tactacagct
     5641 ccatctgtta tatatagagt tacaaaaatg gatggagagg ttgtaatgat acaaaatcct
     5701 gcaaacttac cagaaccatc tgagataaag atgatagaag agcctatagt aaaaggagat
     5761 ataatagttc ctaaagacta tgtgggtgtt gttatggagc tttgccagga aagacgtgga
     5821 aatatgttaa atatggaata tatagatgag agaagagtaa tgcttcacta tgaccttcct
     5881 ctcaatgaag ttgtttacga tttctttgat gctttaaaat cgagaactag aggttatggt
     5941 tctcttgatt atgaggttaa aggttatgta gcatctacac ttgtaaaact tgatatatta
     6001 atcaataaag aacaagtgga tgcacttagt tttattgtcc atgagacacg tgctttccct
     6061 agaggaaagg caatgtgtga gaaattaaaa ggagaaattc caagacatca gtttgctata
     6121 cctatacaag ctgctgttgg taataaggtt attgcaagag aaacaataag cgctctaaga
     6181 aaagatgttc ttgctaaatg ttatggtggg gatatatctc gtaagaagaa acttcttgaa
     6241 aagcaaaaag aaggtaagaa gcgtatgaga caaattggtt ctgttgaagt tcctcaaaaa
     6301 gcatttatgt ctgtacttaa actagatgaa taaattctta taaatttaga taaatatatt
     6361 taaaagctag agtgatttta gaattatttc taaattaatc tagctttttt gtggtatatt
     6421 ttaagtaaac tcatagagaa tataagtaag aatatattaa agaagaataa aaaggtgata
     6481 tttatgatta atatagaaat tcctaaaaaa gtagactata taattaaaga attagaaacg
     6541 aatggttatg aagcttatgt agttggagga tgcgttagag attgtttatt agaaagagtt
     6601 ccaaacgatt gggatataac aactagtgct agaccagaag tggtagtaga attatttgaa
     6661 aaaacaattc caactggtat acaacatggg actgtaacag ttatgattga acatgaacca
     6721 tttgaagtga caacttatag aattgatgga aattatagtg atggtagaca tccagattca
     6781 atagaattta caaataacat agttaaggac ttgtcaagaa gagattttac tatcaattca
     6841 attgcataca attcaaagac aggattagtc gacccattta atggctatga agatatacaa
     6901 aataagtata tacgttgtgt tggaaatcca gtagatagat ttgaagaaga tgcacttaga
     6961 atgcttaggg cagttagatt ttcagcacaa cttaatttta aaatagctga aggtactaag
     7021 caaagtattc ataaaaaagc agatttgata aaaaatgttt ctatcgaaag aatccagaca
     7081 gagtttaata aaatattagt atctgattca tctaaattaa acttattaaa atcaacaggt
     7141 ttgttgaaat ttataattcc tgaaatatgt gaattagaag atgttactca acacaatcca
     7201 tatcacatct atgatgtaca aaaacatact ttaatatcta cagaagtaat tgaagatgaa
     7261 ctgtatttaa aacttacaat gttgtttcat gatttaggta aaaaagtaac taaaacaact
     7321 gataaaaatg gtgttgacca tttttatact catagtagag aatctgtaaa gattgcaaag
     7381 aagattttga gaagacttaa gtatgataat tatactatta ataaagtttt aatattaata
     7441 caatatcatg attatagaat agaacctaaa aggaagataa ttaaaaagtt attaaataaa
     7501 cttgaagatg ttgaattatt tgaagattta ataaaagtta attgggctga tacacttgcc
     7561 aaaaacccaa agtatgcaaa acaaaagata ttaaatctta ttgagtgtga aaaagaattt
     7621 aaacatataa taaatcaaaa ggagtgcttt aatcttaaag atttagctat taatggaaaa
     7681 gatttgatat ctataggtat aaaaccaggt aaagatatag gacatatttt aaataagatg
     7741 cttgaaatag tcataaataa tccagaatta aatgaaaaag aaatactaaa agaaaaagca
     7801 ttaaatatat atacatttta gattcaaata taaaaaattt aattacttta aaaaattata
     7861 gaagaaaata cttatttaga taaaataaat aatttttaga gaatatttta gaagttacga
     7921 ttgtatgatt tttaaacgca tagcatttac tctaggacta tagactaata taaaaaaaaa
     7981 ctttgggaat actaaaacca taaatttata aaattaatat tttatatact gtaaaacagt
     8041 gaggagggac agtatgcata ctcaactaca aaagactatt ggtctttcag cggctctttc
     8101 tactgttgta ggtatggtta taggttcggg agtttttttt aagccacaag caatatacac
     8161 tacaactaat ggagctccag gtctaggtat tatagcgtgg cttttaggtg gttttataac
     8221 aataactgct gggcttacag ctacagaaat ttcagctgct ataccaaaaa ctggtggtat
     8281 gatgatttat atcgaagaaa tatatggaga aaagttaggc tttttaactg gatggatgca
     8341 aactgtatta tttttcccag gtacatcagc agcattagga gtaatatttg ctcaacaagc
     8401 atctgaactt ttaggaatga gcccaaataa tatggcaaat gtacttccta tagcaattgg
     8461 agttatacta tttttagcgt tattaaatat aataggttca tccttaggag gaaaagttca
     8521 gacagtagcc actattggaa agatgatacc tttaatttta ataatagtct ttggatttat
     8581 taaaggtcaa agtagtgaag tattaaatcc atttgtagga gatggtgtaa atgcatcaaa
     8641 tgcacttgga caagtattaa tagctacttt gtttgcatat gatggttgga taaatgttgg
     8701 agctatatca ggagaaatga agtctccaga gaaagattta ccaagagcaa tagttggtgg
     8761 tttatcatta gttatggcag tttatattat aataaatata gcttatcttt gggtagtacc
     8821 agcaagtgaa ttagcaacag ttacttcccc agctacatta gtagcaacaa gattgtttgg
     8881 aaatataggt ggaaaagtaa taactgtggg tatattaata tcagtatttg ggacattgaa
     8941 tggatattta ttaacaggtt caaggatacc ttatacatta gctgaaatgg gaactttacc
     9001 agcatctaaa actcttttaa aagtaaattc tggaggttct cctgtaaatt caatactact
     9061 tataactgtg ttagcctgtg tatatgcatt atctggtcaa tttaatcttt tgacagactt
     9121 aactatattt tcaatatggg tattttatgt attgactttt ataggtgtta tgaggcttag
     9181 aagacaaaag ccagatttac acagaccata taaagtccct ttatatccaa taataccaat
     9241 tatagctata ctaggtggac tgtttgtaat aataaatcaa atattgactt caacaattat
     9301 atctttaggt ggaatattta taacactatt aggtttacca gtttattatt atatgaaaaa
     9361 aagataacta cttaatttaa gttagataaa tttgggtaaa ggtgaaaatt aacttttatc
     9421 caaatttttt atactgttat acaatataag cattatatat tacttgatgt gttattttgt
     9481 ttgatttata atttagtaat tgatgcttta agataattta agaaatgagg aataaaaaat
     9541 gctaggactt tatgtacata ttccattttg tgtgaaaaaa tgtaagtatt gtgattttaa
     9601 ttcttataaa atggacatag attcaaaaaa aagatatata gaagacttaa aaatagaaat
     9661 gaaattatat agtaataaat tatataaaga taataaatat aaaaataaag aatgctgtag
     9721 tctaaataaa aatgacaaaa taacgagtat atttgtaggg ggaggtacac ctagtatact
     9781 aacatctgat gaaataagag aagtatttat aagtataaaa gaaatgtttg atatagatga
     9841 gaatgcagaa ataactatag agtgtaatcc tgggacatta acattagaaa aattaaaaac
     9901 tatgaaagag attggcataa atagacttag tatagggcta caggccattc aagaaaagca
     9961 tttgaatttt ataggaagaa tacatactta tgaagaattt gagaaaaact ataaagatgc
    10021 attaagtgta ggttttaaaa atattaatgt agatttaatg tacagtcttc caaatcaaac
    10081 tctatgtgac tggaaggaaa ctcttgaaaa agttgtacac ttaaatccaa cacatatatc
    10141 agcgtattcc cttatactag aagaaggaac tgaactatat aatatgtatg aaagtaataa
    10201 atttgagctt atagatgaaa atgtagatat agaaatgtat gagtatacaa taaactactt
    10261 aaagtcaaaa ggatataatc aatatgaaat atcaaattac tcaaaagaag gctataattg
    10321 tgagcataat attttatatt gggaatgtga acattatata gggattggag caggagcttc
    10381 tggctatatt aatgagaaca gatacaataa tgtagaatct ttagaagatt atcatttaag
    10441 tttggtaaaa agagaaaaac caatacaaga aaatgaaatt ctttctgaga aagatatgat
    10501 agaagaaaag atatttatgg ggcttagaat gaataaaggc ataaaatttg aagattttaa
    10561 aaaaaaattt ggaatagatt ttagagagaa gtataataag caaatagaaa tgctattagc
    10621 tagaaagctt ataaatcaga gctttgaagg tatacaatta actcaaaaag gaagagaaat
    10681 atctaatagt gtatttatag aatttatgga ataattttta aggtgttgac aaaataaatt
    10741 tataatggta tataatatat ataatctttt tagcactcga acttagtgag tgctaacagc
    10801 taggaggtat aataatatgg aattaaatga aagaaaatta aatatcctca aagctatagt
    10861 taaagattat atagaaacag ctgaagctat aggttctaga acaatatcta aaagacatga
    10921 tttaggtgtt agtgctgcta ccataagaaa tgaaatggct gaccttgaag aattagggta
    10981 tttaattcag cctcatactt ctgcgggtag agttccatct gaaaaaggct ataagttata
    11041 tgtaaactct cttatgagta aaagcgaatt ggatgataat gataagattc ttattgaaca
    11101 atgtatgaat cataatataa atcatataaa agaattgatt catgaaacct ctaagctatt
    11161 atcgcaacta acaaactata caactgtagc agttactaag agtttaataa atcaaagtgt
    11221 tataaagcac atacagttgg ttgctatgaa tgacaataat attttactta tagttgtaac
    11281 agataaaggg gacttaaaaa aagctaacct tacaactaat gtatatttag atcaatctaa
    11341 gttaaatttg atttctgata atcttacaag gaagctttta ggtaaaagta taacagattt
    11401 ggatgataat ctaattgctt ttattaaata cgaaataagt gagtattctg gacttataga
    11461 tgagctttta aatgctttga actcaaatat gaaagaagaa gatttttctc tatcattaaa
    11521 tggagctact aacatattta gttatccaga atttaatgat gtattaaaag caaagtcatt
    11581 tttaaatatg ttggaaaaaa aggaaacaat agctgacata attaaatcaa agggaataca
    11641 aaaagacaat ctaaatataa taattggtag tgataatgat tgtgaactgg cacaggattg
    11701 tagtatagtt acagcaactt ataatgtaga tagagacttg gttgggcgaa taagctttat
    11761 aggccctacc agaatggatt atgctagaat ttactctata ataaattata tgagtttatt
    11821 aattaataga aagtaaagaa attttaaatt gcgaggtgta aagtcttgga taaaaaaaat
    11881 gagcaacagg aagtaagaga agaaaatgat acttctatta accaagaatc ggaaactcaa
    11941 gtagagttag aagaggaagt tgttaatgaa gaatgtgaaa catcaagtga aaaaactgat
    12001 gaaaaagaag ttgatgatga aaatgtaaca gatataaatt ctaagttagc agaaaaaaaa
    12061 ttgcaggatg agttggatga attaaatgat aaatatcaaa gacttcaagc cgaatatgca
    12121 aactacagaa gaagaactca acaagagaaa gagacaatag gcgtatttgc aaatgaaaaa
    12181 ataataactg agttgatacc agttatagat agtatggaaa gagccttaga tgcatgtgaa
    12241 gataaggaag atactatgta taagggtata agccttgtac ataaacaatt aatagacact
    12301 ttggtaaaat ttggagtaga agaaatagaa gcagaatcta aggaatttga ccctaatcta
    12361 catctagcag ttatgcaaga aagtgtagat ggagttgaag caaatcaaat agtaatggta
    12421 cttcaaaaag gatataagct aggaactaaa gttgtaagac cttcaatggt taaagtttct
    12481 tgctaagtat aaattaaagg ttaaattatt taacctaata taaaatatta aaataagtaa
    12541 aacttaatta tataattact cgaggaggat tttagtcatg ggaaaaataa taggaataga
    12601 tttaggaaca acaaattcat gtgttgcagt gttagaaggt ggagaagcac aaataatagc
    12661 aaatagtgaa ggtatgagaa ctactccatc agtagtagca tttacaaaag atggtgagag
    12721 aatagttgga gaacctgcaa aaagacaagc agttacaaat gcagataaaa caataacttc
    12781 tataaaaact catatgggaa ctgattataa agtaaatata gatggaaaat catatactcc
    12841 acaagaaata tcagcaataa tattacaaaa attaaaatca gatgcagaaa gttacttagg
    12901 acaaacagta acagaagcag ttataacagt accagcttac tttacagatg ctcaaagaca
    12961 agcgactaaa gatgctggta gaatagcagg gcttgatgtt aagagaataa taaatgagcc
    13021 aacagcagca gctcttgctt atggtatgga taaattagac caagaaaaga aaatattagt
    13081 atttgactta ggtggaggaa cttttgacgt atctatactt gaaataggag atggaacttt
    13141 tgaagtttta gctacagctg gtaacaatag attaggtgga gatgactttg accaaatagt
    13201 aatagattat ttagcagatg aatttaaaaa agctgaaggt gtagatttaa gaaatgataa
    13261 aatggctctt caaagattaa aagaagcagc agaaaaggct aagaaagaat tatcatcaac
    13321 aatgagttca aacataaact tacctttcat aactgctaca gcagaaggac caaaacactt
    13381 aaatatagat ttatcaagag ctaaatttga agaattaact agaggtttag ttgaaaaaac
    13441 tatggaacca actaagacag ctttacaaga tgcaggactt tctacaggtg atatagatga
    13501 tgtattatta gtaggtggtt ctacaagaat accagcagta caagaagctg ttaagaagtt
    13561 tataggaaaa gaacctcata aaggtataaa cccagatgag tgtgttgcag caggtgcttc
    13621 tatacaagct ggagttttag caggggatgt taaagattta ttattactag atgttactcc
    13681 attatctctt ggtatagaaa ctatgggtaa tgtaatgact aagataatag aaagaaatac
    13741 tacaatacca actaagaagt ctcaaatatt ctcaactgct gctgataacc aaacagctgt
    13801 tgatatacat gttcttcaag gtgaaagaag tatggcttat gacaatacaa ctttaggaag
    13861 attccaatta acagatatac caccagcaca aagaggaata cctcaaatag aagttacttt
    13921 tgatatagat gcaaatggta tagttaatgt ttcagctaaa gatttaggta caggaaaaga
    13981 gcaaaaaata actataacat caaacactaa tttatcagaa gcagaaatag agcaaaagat
    14041 aaaagaagca gaaatgaatg cagaagctga caaacaaaag aaagaaaaaa tagaagcatt
    14101 caatcaagct gaatctacta tatatcaaac tgaaaaaact ttaaatgaac ttggagataa
    14161 aataagttca ggtgaaaaag aggatataga aaaagctata gcagacttaa aagctgtaaa
    14221 agataatcaa gatgctacag cagaagaact taagaaagct acagatgaag taatgactaa
    14281 gttccaaaaa gtatctcaag aaatgtacca aaaagcagct caagaacaac aagcggcaca
    14341 aggtgcagaa caagcacaag acaatggacc taaagatgac aatgtagtag atgctgactt
    14401 taaagaagta gatgaagata aataagctat ataatatgta acactaaatg ataattggaa
    14461 catatatgat ttgtgaaaat catatagatg ttaaagatat atggacaata cacatcataa
    14521 agaatataat atatcataga ttatataact taaagtagaa tataagaatt aatgatagct
    14581 tgtagtttat tattctacaa gctatcgttt tgtaaaatga aggtggtgtc aatttttgag
    14641 tacaaaaagg gattattatg aagtcttagg tatcagtaaa gatgcagaag ctcaagagat
    14701 aaaaaaagct tatagaaaat tagcaatgaa gtaccatcca gatagaaatc ctggagataa
    14761 agaggcggaa gaaaaattta aagagataaa tgaagcttat gaagttctat ctgatgatac
    14821 aaagagaaaa acttatgacc agtttggaca tgatggatta aatggtcaag gtggttttgg
    14881 tggtcaagga ggatttggcg gtcaaggctt tggtggtttt gaagatatgt tcggagatat
    14941 atttggtgac atgtttggag gaagcttcgg aggtggaaga gcaagaagaa gagggcctca
    15001 acgtggtgca gatataagac agagtgttac tatatctttt gaagaagctg catttggtaa
    15061 aaagatgtct ataaaagtaa atagaagtga agagtgtgaa gaatgtaatg gaactggagc
    15121 aaaaccagga acatcaaaga aaacttgttc aacttgtaat ggaactggcc aagttagaac
    15181 tgttcaaaga actccatttg gtaatatagc aagttcaaga ccatgtagtg cctgtaatgg
    15241 aactggagaa gtgattgaat ctccatgtag taaatgtcat ggaactggaa atacaagaaa
    15301 agttaagact atagaagtag atatacctgc tggtatagat gatggtcaaa tgataaaact
    15361 ttctggtcaa ggtgaagtag gagaaaaagg tgctccaaga ggagacttat atattgtagt
    15421 taatgttaag tcacatccac tatttactag agatggaaat gatatttatt ttgaaatgcc
    15481 aattacattt gttcaagcaa ctttaggtga tgagatagaa gtacctactt tggatggaaa
    15541 agttaaatat agtgtgccag aggggacaca aactggaact gtatttagac ttaaagagaa
    15601 aggaatccct agaattagag gtaattcaag aggagaccaa tatgttaagg ttgtagttga
    15661 aattcctaaa aaattaaacg ataagcaaaa agaactattg agagaatttg cgaaggagtg
    15721 tgggtcgaac gtccacgaaa agaagaaaac cttcggacaa aagatagaag atatgtttaa
    15781 gaaaaagtag ttaaagtagc caatatataa tcatattttt gaaatcttag ttatttataa
    15841 gattgtcaaa ggagatatta tgatttttat attggctatt tttgtataaa aaataaatgt
    15901 gcataaaata aaactgaata taaataattt tctgaaaaat acttgtccta aaatatataa
    15961 tgtgctataa tataatcaaa taaaagaaag gggtgattcc agtgggaacg gatgtggcac
    16021 tgccaaatga tgaagaaaac tttattgtaa tatacaaaat aaaattagat acttttagtg
    16081 ctagattaag tactggaaaa actcatgagc tgggataatt attgtaatta cctagactca
    16141 aacttttagt agtaaaatag taatattata tagacaaggt atctaatttt taaatctttt
    16201 gtatgagtta aaatttttat aaagggtata tctgattaga atgtaattag ggaaacctag
    16261 ttgtgaatta cttttagtat atacatgtgt aaagaaatat gtatatggta ttggttattg
    16321 tgctagatgt gtatgtaaag gaatatgtgt ctagttgggt atatgtaaag aaatatactt
    16381 ataatctatg aaaaattttt agtataaatt gctaaaacat tgagattgtt ttttatagaa
    16441 agttagttta taccaactaa aaatgtgctt aagatttgtt tgatgtttaa ttataaataa
    16501 ggttgtagat aaaaaaataa gtaaagacaa aggtttaaaa tacttgcttt aaatatagtt
    16561 tgttttgaac acttgccaaa ttaaaataat taataaaata gacatcttct catagtaaaa
    16621 tatactgtga gaggatgttt ttgctttgtt cataaacttt tatgaaaaat ttgcatagtg
    16681 tcacatttta atatactata tatataataa aatatagata aaattaaatg attcaaaaga
    16741 gaaaggtgaa aagagatgta ttatataggt gtagacatag gaggaacagg tatacaagca
    16801 ggagtagttg ataactatgg gaagataatt tttaggtcag agtgtaaaac agtaattgaa
    16861 aaaggctttg aaggaatact taatgacata aaaataatga tttataagtt gttggaagat
    16921 aataaactta ctatgagtga cataaaatct attggatttg gtgttccagg ttttataaat
    16981 aaagaaggat tagtaacttg tgtaaattta aaatggaata aaaaagcttt taataaagag
    17041 ttaaaaagaa gatttccaga tgctgaaatt catggagaaa atgatgcaac agtagcagca
    17101 cttggagaag caaagtttgg aagtatgaaa ggtgctaatg ttggagtatt atatacttta
    17161 ggaactggtg taggtggagg aatagtaatt aatcaaaaag tcttttctgg agcacatgga
    17221 ttgggttcag aaataggtca tcaaattata ggagaaaact attttaattg taattgtgga
    17281 aataatggtt gtgtagaaac tttttgttca gcaactgcaa ttataaagta ttctcaaaaa
    17341 ctgatagaag aaggagaaaa gagtaggatt ttagatttgg cagagggaaa tttagaaaat
    17401 gtaaatgcaa agatggtgtt tgatgcatat agagaaaatg atttagtggc aattaaagta
    17461 ataaatagat ttaaagatta tttagcaaaa acttttgcaa acacaataaa ttctttagac
    17521 ccagaaatta tatctatagg aggaggcatt tctaaatcta gcgatataat acttgatgga
    17581 atagaagatt tggtaagaaa atttgtatta tacaaaacag aagatatagc tacaattaca
    17641 tgtgcaactt taggttcaga cgcaggaatt ataggagcag cttttttata atgtaaatga
    17701 actgcttatt ggaggcgtga tgatatatgg gtaaaacaaa tgctagaaga acgctttttt
    17761 tgatagcaat aggttcagga actatgctga atcctcttaa ttcttctatg atttcacttg
    17821 ctttacatag tatacaaaat gattttcata ttagttttac aactgtatct tggattgttt
    17881 cagcatttta cctagctagt gctattgcac aaccagtttc aggtaagata ggagatttaa
    17941 taggtagaaa ggtcttattt ttgagtggtt tgatgcttgt aattttatca gcatccatag
    18001 taccacttgt tcaatcattc tttatattga tttttataag agtaattcaa gcaattggaa
    18061 caagtaccct atatccatct ggagttgctc ttatacaaaa taatataaaa gaaagacaat
    18121 cttctgcatt agcagttcta accatttttg cttcaactac agcaggatta ggtccgacac
    18181 ttggaggatt attgttggac ttaggaggat ggcatgctat atttcttgta aatatacctg
    18241 ttgttttggt ttctatatgc ttagggtatt ttttattccc aaaagaagta aaagaaaaga
    18301 aatctataaa agaaacttta aaaaatgttt tgtcaagatt agatattatt ggcatacttc
    18361 tattttcaat tgcaatgatt tttatattac tatttttgct atctatgaag gaatctttta
    18421 acttagaaca acttgtattt ggtataatac tgatgtgtct ttttatttgg catgaattga
    18481 gaacaaaatt gccatttata gatattaagc tttttgtaag taatcctaaa ttgtcaaaag
    18541 tttacttgca atttattata ttaaatttct ttaattatat tttgtttttt ggtcttccta
    18601 gttattttca agatgcctta cattatagtg caaagtcgac tggattattt atgctattta
    18661 tgtctggtat aggtatattt atatctccat taactggaaa atggatagat aaatctggaa
    18721 ctagatttcc tcttgttaca tcatcaatct ttatgtttat tagtgcagta cttctttcag
    18781 tagtttttat ccatatacct gtaataggaa agggtattat attatcatta gcaggtatta
    18841 gttatggagt tggtaatgta gccctgcaat cctctatgtt tgaagaaagt cctcgtgaca
    18901 gtatagggac agcttctggt ctgtttcaaa ctagtcgtta tttaggttct attttagcaa
    18961 ctgttttact tggaatggta tttgaaggaa atataacatc agaacaattt cagatattag
    19021 gttatgttat ggtggtctta ggtacaataa gttttttatt aaattttata ttaaagaaag
    19081 aattaagaaa agtagaatag tggatttatg gatttaataa gtctaataac tgataagttt
    19141 aaaaatattg tttgtaaaac ataactggtt tgatatttat taattctaag ttttgaagca
    19201 tagagtactc gataagtata caatattttg aattaaaatt aagcagaaat ttgaactagt
    19261 ctttcaaatc tctgcttaat ttagtagtta aattttttta tagagaaaat aagatttgac
    19321 acttagataa aaaatcattg cattttcaaa attctttaaa tcaaacccaa gtatctcatt
    19381 aatcttttcc aatctatata ctagcgtgtt tctatgtaaa aacatactag tagatgtctt
    19441 agaaatattt aaatcattat ttataaattt tattcctgta tatagaagtt cttcgtcaat
    19501 agatttaatc ttattaaaat ctattaattt attgagtata ttatcctcat taattaaatc
    19561 taaagtttta tatctaataa ggtcaaacaa atttaaaaac ttattttcag cattgtcttt
    19621 tataatatga ggtagtaaat cagaacataa ctgtaatcta aaataaaaat cagctttact
    19681 atctaattca ccaagataaa aaatataagt actttgataa gtttcagttt caattatatt
    19741 ttgtgtagtt acattaatat ctaaattata tttagaattt actataaaaa aaccattgtg
    19801 ttgtattata tttatatctt caaataaatt ataaaaaata
//
LOCUS       DAGQWR010000081        19192 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.081, whole genome shotgun sequence.
ACCESSION   DAGQWR010000081 DAGQWR010000000
VERSION     DAGQWR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.081"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     repeat_region   30..324
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=30..58
                     /rpt_unit_seq="ctttacattccatatagttaatataaaac"
     gene            complement(1155..2027)
                     /locus_tag="MIW95_002415"
     CDS             complement(1155..2027)
                     /locus_tag="MIW95_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY3151736.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(2421..2999)
                     /locus_tag="MIW95_002416"
     CDS             complement(2421..2999)
                     /locus_tag="MIW95_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBY3151737.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(3057..3773)
                     /locus_tag="MIW95_002417"
     CDS             complement(3057..3773)
                     /locus_tag="MIW95_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBY3151738.1"
                     /translation="MRINKYIASCGIASRRKSEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            3964..5049
                     /locus_tag="MIW95_002418"
     CDS             3964..5049
                     /locus_tag="MIW95_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBY3151739.1"
                     /translation="MIIILKENIKYYLIVVFIGILFFKFINTPSNFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(5204..5863)
                     /locus_tag="MIW95_002419"
     CDS             complement(5204..5863)
                     /locus_tag="MIW95_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151740.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KYSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6080..7372)
                     /gene="lysA"
                     /locus_tag="MIW95_002420"
     CDS             complement(6080..7372)
                     /gene="lysA"
                     /locus_tag="MIW95_002420"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBY3151741.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(7390..8583)
                     /locus_tag="MIW95_002421"
     CDS             complement(7390..8583)
                     /locus_tag="MIW95_002421"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBY3151742.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(8944..9561)
                     /locus_tag="MIW95_002422"
     CDS             complement(8944..9561)
                     /locus_tag="MIW95_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151743.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(9731..11431)
                     /locus_tag="MIW95_002423"
     CDS             complement(9731..11431)
                     /locus_tag="MIW95_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151744.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(11828..12391)
                     /locus_tag="MIW95_002424"
     CDS             complement(11828..12391)
                     /locus_tag="MIW95_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151745.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(12550..13263)
                     /locus_tag="MIW95_002425"
     CDS             complement(12550..13263)
                     /locus_tag="MIW95_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBY3151746.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            13555..15219
                     /locus_tag="MIW95_002426"
     CDS             13555..15219
                     /locus_tag="MIW95_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBY3151747.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDSKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(15326..16225)
                     /locus_tag="MIW95_002427"
     CDS             complement(15326..16225)
                     /locus_tag="MIW95_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBY3151748.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(16252..16890)
                     /locus_tag="MIW95_002428"
     CDS             complement(16252..16890)
                     /locus_tag="MIW95_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBY3151749.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KVGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(17227..18225)
                     /locus_tag="MIW95_002429"
     CDS             complement(17227..18225)
                     /locus_tag="MIW95_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBY3151750.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(18323..18898)
                     /locus_tag="MIW95_002430"
     CDS             complement(18323..18898)
                     /locus_tag="MIW95_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151751.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         6239 a         3211 c         1996 g         7746 t
ORIGIN      
        1 tttatactaa tgactgatgc tagatttaac tttacattcc atatagttaa tataaaacta
       61 ttataaggag cattatacga ccaaaagcca aagcagcttt acattccata tagttaatat
      121 aaaacaagca acagcaagag tcgcgacaac attaaaaact tcctttacat tccatatagt
      181 taatataaaa cctttatttt tttctctata tcgagttcgc caaattcact ctttacattc
      241 catatagtta atataaaaca gtccagcgtt gtgtggtcgt aacacaccaa tcctacttta
      301 cattccatat agttaatata aaaccccaaa ataaattaag tatttccaat acttatacat
      361 atacaattct cctaaacttg cagtgagcga gcaatagtgc aattgataac agttatcata
      421 cactctcaac gctttatatt tcaactatta aaccctactt tatcacaaaa atcgctcact
      481 gcaaaacttt tatattttta ttatatcata aaactcatac tataaaataa aaatgggata
      541 attttacaaa ctaaaagcat ctacattctt ataagtattt attatattct ctattccaat
      601 gattcctttt tctttttatt gcatccttcc tatgtaatcg cactttagct caacaagtct
      661 tttattttca ttaacaattt tttaaactat tcaaaatcat caaatttttc atcttataca
      721 gttaaatcaa aatacgtgtt atatctcttt atctatatac ttttcttcct tccatttctt
      781 tttctctttc tacattttca ctttgttact ttacaatctc aaggttatta tctgctatta
      841 aagttttatc tttccaggtt tcacaaaaat aaattgaata agtgcaaaaa taacagatac
      901 aaaaccataa ctattttctt ttctataaaa aaatatatca tcaacaagaa acataataaa
      961 tgaaaataac tatgaataaa tactctcatt tagaagaaat agacaaagat attccttaaa
     1021 atttaagcaa aatattgttt aaataaaaaa actgactgtc agttaaatgc cagtcagcta
     1081 ttttatatat tttatccaaa catcactata tttgaacatt ttaataatta ataatactta
     1141 ttaatataca aactttactc atacacagaa tatcttttcc aaatttgctc taaatcatca
     1201 aaatgctgtt taatatcttc tttttccttc attccaacag atatagcaag ggcatttgaa
     1261 atactaagag ctggaactag agaatctaca aatgaagcca tattactctt aactagaaga
     1321 gtgttatctg caagtgatgc tactggcgca aataagctat ctgttaatga tattacatgg
     1381 gctccttttt ctttagcata attgacaatt tgataagatt tcttagaata acgagggaaa
     1441 ctaatagcaa ctattacatc ttcttcattt attcttacaa tctgctcaaa agcatctccc
     1501 atatccattc taattatatg aacattgtca agtattatat ctaagtaaaa acctaaatat
     1561 tgagctacta caaaagaact cctcattcca agtatatata tttttctcgc tcttaaaagt
     1621 ttgttagaag cttctttaaa cgctctctcg tcaatttctt ctagtgtaga ccttatatta
     1681 tctatatcac tttttaaaac tttgtttagt attgcaaaat catcggaata atcatgtgcc
     1741 atttcaactc tttgtactgt agttagttta ttttttatga gttcttgaag tgcagcttgt
     1801 aatttaggat atccactata acctaatgca tttgcaaatc ttacaactgt agactcactt
     1861 actcctacgg tttcacccaa tttacatgca gtcataaatg caactttatc ataattattt
     1921 aaaatgtatt gtgctataag tttttgaccc ttactcaatc tcgtatattg agattgtata
     1981 tttgatatca aatgtttact gtccttcatt tcatttgttt cttccatttt tttctccttt
     2041 tagttatata aactattttg ttttattaac actgtctttt tctctaaaaa ttttattttt
     2101 tatctcaatt tcatcttagt ttttatcata gcttcttaaa ctcataaaat tctaactttt
     2161 ctgtacctta tacttcacat agtattttcg attaaattta ttataccatc tttccttgca
     2221 ttttttaata tttatccaat atttctttta aatttttcaa tttttactca tttaaaagtt
     2281 atctatctta ttttgcatat ttagatgtat attttgtttt gagaataagc tgttgtatac
     2341 ttattattta aagtattttt aataggtcac actatatata ttaactctta atagaatctt
     2401 gtcaagtttt ttagtattta ttatgctttt ttcttttcaa ttaaaatcaa ttgtggtggg
     2461 ttattgactt gatttaaaaa cttagattct aaaacattaa attcatcttg atttaaatta
     2521 ttgacaaggt tatatatgca atctttttct tcttgacctc catcatgccc tgtatatatg
     2581 gcaatgctta ttactccatt ttcttttaac atttctaaac ttaactttat agcctttaaa
     2641 gttgtatctg gtttggttat aattgaatgt ttcgctcttg gaagataacc caaattaaat
     2701 aaaacacatg aaacttcttc ttttatatat tgattcattt tttcatgccc atcaaatatc
     2761 aactttactc tatctgtata tccttctttc tctagtttct ttcttgtaga ctttattgct
     2821 tcttcttgta tatcaaaagc atatacaaag cccttttctc caaccttctc agctagatac
     2881 cttgtatcat acccatttcc catagtagca tcaattacta tgtccccttc atttataata
     2941 ttttctaaat aaaatttatt tatatctgta attttagtta aatactttgc atatgccata
     3001 atactatttc tccctactat taactttatt tcattttttc tcaatttatt tttatcttat
     3061 tttattgatt ttaaatattt tatttcatct tctgttaggt atctatattc tccaactttg
     3121 gtgtctttta atactatttt ccccacagaa atccttctta aatttaaaac tggatgattt
     3181 atagctttgc acatttttct tacttgtcta tttctaccct catgtatagt tatttcacac
     3241 actgaataat ttttttcttt attttcttta gttaccctta ttttggcagg tgccgttgtg
     3301 taatcttcta tttttagtcc tgtttcgaat tgtcttatct catcatcact tataattccc
     3361 ttcacacttg ccacatacgt tttatcaatt tcatgctttg gatgggtaag cttatatgtt
     3421 aaatcgccat cattagtaag tattaataaa ccactagttt cataatctaa tctccctatt
     3481 ggatatactc tttcccttac atctttaagt atgtctatta cactgggtct attgaattgg
     3541 tcttttactg tagttatata tccctctggt ttatttaata ctatatatat ataattttca
     3601 cttggtttta cttttttatt atcaaattct actatgtctt tttcttcatc aatattaaaa
     3661 gataactctt ttactacact tccatttacc tttattcttc ctccaagaat tatttcttct
     3721 gattttcttc ttgatgctat tccacaagaa gctatatact tattaattct catacttaat
     3781 ccttccttat cttaaattat gtgtgtttaa tatttttatc aatgttaatt ttgctacatt
     3841 attatactat actagacctt aattaaaaac aagcaaaaaa attatttaat tttcctaaat
     3901 aattgtaatt tcttttcaca tactattaat atgtaatagc tgactaaaca ctattttaag
     3961 gaagtgatta ttattttaaa ggaaaatata aaatattatt taatagtcgt gtttattgga
     4021 attttatttt ttaaatttat taatactcca tcaaacttta tatctagtat tgaaggattc
     4081 ttaaagtttt ttagcccatt tttattagct attttacttg ctttactttt aaatccactt
     4141 gtgatgcttt ttgaggtaaa attcaaagca catagacttt tagccatttt tttatcttat
     4201 atatttatag gttttatttt agcctttgcc attaggctat taattccatc aatagctaat
     4261 acattaaata gattgataaa tgaaatgcct atgtatactg attatataga tagttttata
     4321 gaaaaaaata tgtcaaacat agattttcta aaaactttaa ttcctcatat acaacatagt
     4381 ttagataatt tattaaaaga agcaagtaat tttgttggta aaattcctaa aaacttctta
     4441 atctatacac ttagtatatc ttctatgcta tttaatatga caatgggatt tatactatcc
     4501 atatacatta tatatgacaa agaaaaaatt gcattgggat ttaagagatt tctatattct
     4561 tctactgcta gaaacaaggc agataacatt atagaatttt ttaggactac tcatgatatt
     4621 ttctatgatt atttacttgg tagaatatta gactctctta ttattggtat aattgcattt
     4681 ttaggatttc aatttgttat cagaatagaa aatgctttat ttttagcttc aataatattc
     4741 ttaggtaata taattcccta ttttggtcct tttattggtg caatccctcc aatagccatg
     4801 actatattat atagtccaca aaaaactata tgggtcattg catttctatt tatattgcaa
     4861 caattagatg gaaattttat agaaccaaaa gttatgggta atcaagttgg tataggagct
     4921 atatgggtaa tatctgctat tttggtggga caatctctat ttggtttcat aggagttttt
     4981 ctttcagtac caatagctgc tgttgtaaaa acttatgttg ataaatatat agataatcgt
     5041 ttacaatagc ttatctctac tgtgacttta atttttagtt taaaatttaa ctgttattta
     5101 ttaaattatt tctcaaaaaa tatctcttag taagaaaaaa ttgctaagag atatttttta
     5161 taatatataa tttgttcatt aaatttatgc atatatttta tacttattga ttatttgagt
     5221 tttgagaatt tccaccacta tctgaaccac cagtagtatc ttgtggagtt ggtgctggtg
     5281 ttggattact attatttggc ttttcttgat tttgagaaga actgttatta ttgtcagact
     5341 tattactatc tgatttttta tcattatttt tagaactata tttattattt ttactacttc
     5401 cactataaga agatttcctt gcatctgatt caactctata cctatcttct ccagccgtat
     5461 cttttgcctt tttctttctt tgttcctctg cttcgttttg ttctttttct aatttagtta
     5521 actcttcttt ctttttagtt gcctcttctt taataacaga ggaaccattt ttgattttct
     5581 ctataagttt aagttccttg attgcaccct cataattttt taattcttca aactccgtca
     5641 tatttgacat tctcatagct tgcatataca tagctcttgc attctcatta gtactatctt
     5701 cttccagtgc agaggataat gattccatag cttttttata gttatgtttt tctaaatatg
     5761 tttttccttg ctctactaaa tttatttcat cttttttatt aaatgaacat cctacaagaa
     5821 atatagataa tatcgatatt aataaaatta ctcttcctct cattaaatcc ctccttgtca
     5881 tatactttaa attttatcat aattataaag tttttaaaat attttatatt tattacaaga
     5941 aatttgttag taaaacaaaa aataagcaga tatgctacga acgatacaat aaaccaaaaa
     6001 ttaatttaga gatacatatc ctagcaaatc tgctcataat aaactttaat atttaatttt
     6061 tattatatac ttttccattt tatatctcta aagctaataa gtcctcatag ctttgtctct
     6121 tacaaattat cttatcattt ccatcaaata ctgatactac tgctgctctt ggtattttat
     6181 tataatttga agacattgaa tatccatatg caccagttga tgttgttata agtatatctc
     6241 cacttttaat atccattatg tttatatctc caattaatat atctccactc tcacaacatt
     6301 ttccagctat tgttacatga ttcatagcct catgattaat tttattcaca atactacact
     6361 cataactagc ttggtacaac gaaggtctta cattatctgt catacctcca tctacactta
     6421 cataaaccct aacatctttt atatctttta tagaacctac tgtatacaat gtacttcctg
     6481 catttgcaac tattgatctt cctggttcta ttaccaatgt aggtacttct attccaagct
     6541 ctttacatga agtttctgct ttagtgatta tggtttcaca aaactctttt atagttttag
     6601 gtttatctcc ttcgctataa taaacgccaa ctcctccacc taagtcaatt tctgttagtt
     6661 gtatgttgaa tttttctttt atttctttta ccaagttcat cattatatcc actgtatcta
     6721 tgtatggttc tacatcaaat atttgtgaac ctatatgagc gtgaagacct acaagtttaa
     6781 tatttttata ttcacttaat ttttctattg ctctaaataa atctccattt actaaagcaa
     6841 atccaaattt agaatctatc tgtccagttt ttatataatc atgtgtatga gcttctatac
     6901 caggagttat tctaaagtat atctcttgag ttttaccttt atcttcacat aatgcttcaa
     6961 gcaaatctaa ttcataaaaa ttatccacta caaatttacc tacacctaaa tctaatccca
     7021 tgtttatttc ttctttagtc ttattgtttc catgaaagaa aactttttcc ataggaaaat
     7081 ttgatttata agctgtatat aactcgcctc ctgaaactac atctaaacac atttgttctt
     7141 catttataag attacacatg taaagaggta aaaaagcttt ccctgcataa gcaaccttat
     7201 tttttccctc tctagcctta aaatattcta tatattctct acaattttgt ctaactaaag
     7261 cttcatcaaa tacataaagt ggagtttgat attttttact aagttctaaa caactcactc
     7321 ctccaatata taattcatca ttatttacag tcatagttcc atgaagtttc ataatagcct
     7381 ccccaaattt tatatattaa atacttctgc tattttttct atagctactt gcttatcatc
     7441 agaatttata gcacaagtta tacgaatctc tgatgtagtt ataagtttta tcattatacc
     7501 attttcattg aatatcttaa atactttagc agcaactcca gaagtatttt tcataccaag
     7561 acctactaaa gaaaatttag tgatattatt atcaatcact acatgttctt catctgtata
     7621 tttagatact atctttttac attctcccaa ctcttctttt ggaactgtaa atgatacact
     7681 tatcttatct aaaataggtg cagtttggct tatcatatct acacttatac ctatagttgc
     7741 tatatcttca aataagctag atatattttc agctttaaaa tcttttattg ttatagaact
     7801 gtcatcatca ctagtagcta atcctgttat tactttatcc tctaatttca tgtcttttcc
     7861 ccctcttata tatgttcctt tttctgtacc acatgtacgt ccgacataga tttcaacacc
     7921 atatttttgt gcaagctcta tacttcttga atgcattact tgtgctccta aactcgctaa
     7981 ctcaagcatt tcttcatatt caatctcatc aagtttacta gcctttgaat attttcttgg
     8041 gtctgtaaaa taaattccat caacatctgt atatatctca cattttccat ttaatttaac
     8101 cgcaagagct acagcagacg tatctgaacc acctcttcca agagtggtta catcaccatc
     8161 ttcgttgatt ccctgaaatc cagttactat cacgacattt ccttcatcta aactattttt
     8221 aattctgttt acgtttatat catcaatttg agactttcca tgaagaccac tcgtttttat
     8281 atttaactga taagcattat aacttatagc tttacatcca agtgcattta atgctattga
     8341 taataaagat gctgaaatca tttcaccagt agacattaaa gcatctaatt ccctcttact
     8401 aggttcatta ctcaattcct ttgctaaagt tatatattca tcagtggatt ttcccatggc
     8461 agacacaact attaccattt gtggattttc ttttcttcgt tctattatat tttctgctat
     8521 tgattttatt ttttcagtat ctgctactga acttccacca tatttttgaa caattatact
     8581 catgctttct ccccctcaag ctaaattttg ataaataaaa acgcccaaag gtatactttg
     8641 gacgtgtaaa tttcatagac caagtaacct tgtagctcac cactttaaaa gtgacagcct
     8701 tatgcatatt ctgcataagt ccagaaaatt tgtccgtcaa caaaatttct tcggctatat
     8761 accctttcat caattatcaa tgcttaccag ctcctattga atactattga atacagctac
     8821 ctctacccta ggctcattat ttttaattaa tttattaata tagttttatc atattctata
     8881 taatattgca acaattattt tacaaaatgt ggcaaatcga tagttttctt ttctaaagtt
     8941 agcttatata caattaaata gtttatttaa tcctaaatct ataaaattta tgctatactc
     9001 attatcatat ctctctaaat catttaattc ggatagatta tctggtgtaa ctaccaaaac
     9061 gtttggtact ttaaaaaatg gaattgtaga tttatctatt tttttagaaa gattttcgta
     9121 tttagaaatg tctatttcat tatttaagtc aacatcaact aaaacttgct ttatattacc
     9181 aaattggtct gtatattcaa taaataaatc gtatttcaca tcatctacac taacatttcg
     9241 gtaaaatctt tttacatcac aacctgcagt attgattcta acaactaatt ctgatcctac
     9301 tagagccttt attaaatcat ctcccttaaa aagtttacta ccgtttaaat aatatacata
     9361 agagaggtct ttataattat aataatccac atcacaagga tattttttta gtgtatattc
     9421 actacacatt tttctcaaag tttttttaaa atttttttta ttgtatcttt tttggtctcc
     9481 ataaattttt ctaaattgag actctgtaac acatctaacc ctgctaatta aaagtaatat
     9541 ctctttttcc atattcgtca tagtacattc tccttgaagt attatttgca aagttgtact
     9601 aatattatat taattttatg acattaaatt tacaaagaat taaaataaaa tagctaattt
     9661 ttatagaatt aaattctata aaaattagct atcactatgt taaatactat tatcaatctt
     9721 tttatttaaa ttatacatta ccttgatttt cagaatcttt ggaatctgta ttttcatcag
     9781 ttcctgtttt ttcttttgct tttttatctt caaagtttgc aatatttagg ttatcaaatt
     9841 ctgacatttc tattacacca gattcttctt ttgaatacga tattttgact ttatcaccaa
     9901 cctgagtcaa tggcaattcg ctagatactt ttgaagtagc cctaaatatt atatctttat
     9961 ttgaatctaa agttacataa taattagtgt taccacctcg cacatctggg cttattcttg
    10021 tcactgtgcc ttctaatacc tcatctttta aatcattatc aagttttaaa tcattaccct
    10081 tagattctaa tgcatcctta tagtttctta atgcttcatt cttatcttca cctattccaa
    10141 gtacattata atcttcaaca gatacaaatg caactctctt aacaagacca gccttatctt
    10201 ttaatgatga tacatatgtt ggctgaccca atatattata cattacaggg aacgtcgctt
    10261 cataattttt ttcttgaaca ttaccttcag ctgatttcat tgcagccgtt tcagtagctc
    10321 ctggttgttt atataatcta gcttcttttg ttctagaatc aactaacata aatccaattg
    10381 tagattcatc tccacctgaa gatgttatac ctgtatacca ataagacctg ttatcattgc
    10441 cataaactaa tgatgtacct tctgttggta ctaaaactcc cttttctgat ataacagagt
    10501 ttaaaaatcc attaacataa acaccccagt ctttaatctg gtctgttaca aagctttgtg
    10561 gttgtattct atccacccat ttaggtgtgt ttttaacatc ataaacattt atttttccag
    10621 tttctgcatc aacagtggct attcctgttg catttgctcc tccatagcca attttatgtt
    10681 catataaact tacaacccaa taaggtcttc cttcatcatt tatttcaaga gtgaaatctg
    10741 tcataccaac atttacaata ccatgtatgt ataaatgtct ttgtaaatcc tgatgtaagt
    10801 aggcttctgg ttggtaaact atctttattg gctttccatc tatttcttga actaattgga
    10861 cgtcttgagg attactagct gatactttta cataaccact tgtaccactt agagatgtta
    10921 tccatttaat tatgtctctg tgaacaagag gcgctaccca gtacaactct ccatctacat
    10981 tttgtatatg aaattttcct aatttggata cactccctat tgcaggaact tctccaagct
    11041 ttttgtcacc taatttcatt gccatatctt catctacaag acgaatgtca tttacactga
    11101 cagggctaat gtcatcagta aatttacttt cagttacttt tcccaatagt tctctatatg
    11161 attttgcatg aagtacaggt gtagaagtta taaagggaac tactacaata tacacaagca
    11221 aagctattgc tattgaaaaa ttatatttag ccattttaga tgctctttca tttctatcta
    11281 acatcatatc tagtattcct gctacaacaa agaaaattat tcctactgaa aataaagatg
    11341 taaaatctaa tcttagtaca ggtaatttaa taaatgcata aattattaca attattaatg
    11401 caactccata tgttttaaga tatcgtttca tttaatactc ctctctaaaa aatgttatta
    11461 ttattattat atccatatat ttacaaaaaa taaatattta tcatatttat tatatattaa
    11521 gatatcttta tatatttata gttctgctta ttcaactata tttaacatta tacgtgtatg
    11581 tataaatact ttttatacac tgtttgtgct atccatcccg cacctaaagg agtgggattt
    11641 ttgcactatt ctgtattata tatatccaaa aatattataa aaatacaaat attagcagaa
    11701 atttaaaaac tgaagaaagt attctaacta agatttacaa attcttaatc ctatcgaagt
    11761 taaactacta tcttcagcct aattatctag caatattttt ttatatgaaa aactctaaat
    11821 caagttttta aatgtttgcg atttttgata cataagaatg ttgtctagaa ttatattctg
    11881 ataataaacc ttcatactct aaggttaagc cagataaatt ataaaaatca tttggcgtta
    11941 cttcaaatgc ttgtttattt aaaatgtcta tctcactttt ttctaacatg tcagcaacaa
    12001 agtttgaaca tacatattta tattcagatt gtctctgaat attcattagt aaatatatta
    12061 atgccattgt atcgtaatag taatcttctc tataatcact aattaatttt atatttttat
    12121 atagattttc atattgtaaa ttatctactt ctaacgaata taccctacac acagtgtttt
    12181 ttcttatagc atacaatcct tgatttatat tttcttcaac aaatcctcca ataaatggat
    12241 tccttggatt tagccttcca aaagaataca ttggttttag attttcattt aatgatattg
    12301 acacatgtgt atacagcttt ttacttatat tccttattaa gtaagataat atagttcctg
    12361 tatatgttgt tacaatatag actttattca tcatttcccc ccaatctagt aactaaatga
    12421 tattatacac ttacaattta atatcataag atattctaac ataataaatg tggttttaca
    12481 aattttgatt ttaaggtact ttaatattat ataatataat aaaaatataa atataagttt
    12541 tctatatttt taaccaacag atgttttctt caaattaatc agatgattta gtatatcttg
    12601 aacctttcca gagtccttta aatatcctaa tgccccattt atttgtgatt ttaaatctat
    12661 ttctacttcc atatccaaat caacatttcc tttttttata gatatatttt taagtgttat
    12721 tgggtaaaga taactttttt ccaaagtaat cttatttcca ttaacctcaa atggaacttt
    12781 tttattgtag ttacttaata ttttttctag cattttatca cttagtttaa attttccaat
    12841 tttcacattc tcaagactta caactaagtt tccatccttt aaagtaggtc tcaattcaag
    12901 ttcatattga ctatttacca aaccaaatac cttatatggt cctgctacct ttattttacc
    12961 atttttcatt tcaacaaaat tattttcaag ttcattaatt ccatgcttct gcatcaatgt
    13021 atatattaaa tttttaaact cttcctctga aacagatatt tttccaacca ttctaagtgg
    13081 attttttact acctccatag aatttatata tgagcctttt gataaatatt cttcatctat
    13141 gattttatta ttttgtgaac taacatcata tctttctttt ggtgtcaaga tatatacaac
    13201 aattcctgca agtactaaaa caataacaat taaacttaac aaaatctttg caacctttcc
    13261 cataatcgtc tcctttacaa atctgtcatc agatttaaaa taaatttaga taactaactt
    13321 cacataatat tcttaacaat atacatacta taatacagtc ttttaaaaat taattcaacc
    13381 aatctaatgt tttgtaaata aactgtaaaa acatgttttt agtataaaag ttaatatttt
    13441 gcataatact cttaattaca cctttatttt tttctaatct tggattaaaa ttaatattat
    13501 gcaatacagt gtagtgttct gtattataat caaattaaat aggaggtata gaatatgtca
    13561 aatgaaacga actcatctaa ctttataaag aatattatta taaacgactt agaaacagga
    13621 aaacatgaca gtatcataac tcgttttcca cctgagccaa acggatattt acatattggt
    13681 catgcaaaaa gtatatgtct taattttgga ttagctaagg agtttaatgg aaaagctaat
    13741 ttaagatttg atgatacaaa cccattaaaa gaggatgttg aatatgtaga gtccataaaa
    13801 gaagatgtaa agtggttagg atttgattgg aacgagttaa attttgcatc taactacttt
    13861 gatgaaatgt ataagagagc tttaatacta ataaaaaagg gtaaagctta tgtatgtgat
    13921 ttgacacaag aggaaatgag agaatatcgt ggtactctaa ctgaaccagg aaaagaaagt
    13981 cctcatagaa acagaactat tgaagaaaac cttgatttat ttgaaagaat gaaaaatggt
    14041 gaatttaaag atggtgaaaa aacattaaga gccaaaatag atatgtcttc tccaaatata
    14101 aaccttagag acccaattat atatagaata tctcattcaa cacaccataa tacaggagat
    14161 aaatggtgca tatatcctat gtatgctttt gctcatccaa ttgaagatgc aattgaagga
    14221 ataactcact ctatatgtac tttagagttt gaagaccaaa gacctcttta tgattggttt
    14281 gttaaagaat gtgagatgga aaatatacct agacaaatag aatttgctag acttaatata
    14341 aataatactg tcatgagtaa aaggaaatta aaacaacttg tagatgaggg tattgttgat
    14401 ggttgggatg accctcgtgt tcctacaata tccggaatca gacgtaaggg atatactgca
    14461 gaagcattgc gcaatttctg tagtgaaata ggggtatcaa aagttaactc tacggtagat
    14521 agtcaaatgc ttgattattt cctaagagaa aacttacaac caaaggctcc tcttactatg
    14581 ggagttttaa gacctttaaa acttattata actaattacc cagaagataa gattgagatg
    14641 cttgaaatag agaataatgc taaagatgaa tctcaaggaa agagattagt tcctttctct
    14701 agagaattgt atatagaaca agatgatttt atggaagaac ctgttaagaa atattttaga
    14761 tttttcccag gaaatgaagt ccgtttaaaa ggtgcttatt ttgtaaaatg tactgatgta
    14821 attaaggatg aaaatggaaa tgtagttgaa atacatggta cttatgaccc tgaaactaag
    14881 agtggctctg gctttacagg acgtaaggta aaatctacaa tacattgggt tgatgctaaa
    14941 tcagctatac catgtgaatt tagattattt gaaccattaa ttcttgatga tatacctgaa
    15001 aatgaaggta aacatttctt agaacaaata aatcctaatt cattggaaat attacaaggt
    15061 tttgttgaac ctacacagat aaaagattct aaaccttttg ataaattcca atttgttaga
    15121 aatggattct ttagtataga taataaatat acaactgacg aaaaatttgt atttaataga
    15181 atagttcctc taaaaagttc ttttaaacca ggtaaataat cttacaatca aaaaatgaag
    15241 acttaaaata taaattactt ctactataaa taaacttagt tagaagtaat tttttatttg
    15301 agtcttcatc atttacttta aatgattaac ttttttcact acagattccc ataacttcta
    15361 ttccatcttt tatgcattct atgttaagag gaaaatctgg tcctttttct ccgtcaatat
    15421 ctgttataag tgaatcttca cattctatat gcaccttttt agttttaaag tatagtattt
    15481 catttccatt atattgatct aaataatcac ctctaagcac acttatcaaa actggtattg
    15541 attttggtat tggcattcct ttaaaaataa ttacatccaa atatccatca tctatttcag
    15601 ccttatatgc tagatttata tttcctgctg ttttgccatt aaaaatcaac ataagataca
    15661 tatctccatc ataatacatt tcctcagaag atacttttat cttaaatttc ctcatataca
    15721 atgcttcttc tattcccttt atataataag aaattcttcc aaatgaattt tttaagtctg
    15781 tgtttatcct ttgtgataca tctgtaaaca taccagcact tgcaacattt ataaagtact
    15841 tattattgac tttccctatg tctattttct ttggttttga atttatgatt tcatttatag
    15901 cttcttttat atcaaatgaa atttgaagag catttgaaaa atcatttgca gttcctgtag
    15961 gcaaaattcc aattggaatt tttatgtcta attctttcat tgcatttact actaaatcta
    16021 cagtaccatc tccaccagct atcaatatgt gttcataaga actatcaact tccttaaatg
    16081 cattctttac aggttcttta atagttaatc tatagggtat caataaataa tttttttctt
    16141 gatataattc tatgatagtg tctaaatttg ataatatctt tttttcacct gaattaggat
    16201 tgtatataag ttttactttt ttcattattt ctccttactc attttattct tttagtatcc
    16261 aaaatcttca tttaaaaaaa tcacatgtat tctttctggt gttaattgtc tttttataag
    16321 tgtataacta cagcatattc ttcctggact tgaacagtct atacatatac cagtagtctt
    16381 acatccagta tttgtactta atcttttaca attggctgga gcagctattg tttttgttct
    16441 ttttatagct tcatctacat ctttaactat tttatttact cctgctataa ctattacttt
    16501 atcaggtcca tatatcatag cagcaactct attcccattc ccatctaaat tgtaaagttc
    16561 tccatcctct gttatcgcat tagtacttgt aaaataagca tctgcagaaa atgactttct
    16621 atatatttct ttcatttctt ctggtttgat atcttttgca tatctatcaa gaaaattata
    16681 tttttctctt ataaaatcaa tcactcctgt ttcaaataag gtcatagaac ctccacatcc
    16741 tactagagaa cctactttaa ctatttcttc aattttttta agaagctctt ctttgttact
    16801 tactaaatac cctttcatat tattgttttc cagagatttt attgtttttt ctattctttt
    16861 ttcattaagc caatttaaat tttgatccat tttaacttcc cccttatttt taatatgtac
    16921 ttaattattt ataaacttta ttgtctttat aagtttattt tagtacatct aaaaaaaaat
    16981 acaatataaa acttaaatag ttttatataa ttttttccaa aataacttac tcaatcaaaa
    17041 aaggaaagta gatgtactat tctactttcc ctatattgta tgtagtattt attgcctaaa
    17101 atctatatat taattatatg tttcatatta tataatatca cagttaatta aaatgtaata
    17161 ctatcttctt tttaaacata taaattgtat tattaaattt aatatatcca acttcaataa
    17221 atcttattaa tttgatgaag aagatgctat agctgcactt gttgctgcca ttacacatgc
    17281 aatttgaata gcaacaacaa tatttaaaaa tatattatta gttatagagc ctatattatt
    17341 ctcatttcgt atcaaatctg cataaattga agctacaata gcagaactaa tcattactct
    17401 attacttttt cctaaagacc aatttccaaa gcctttttct tctttaagtt tatttgaaac
    17461 attttttatc tcttctatta tttcatcttc tctataatct aataatgaaa ttgctcctat
    17521 tattggatat ccataataat tcattttaca atcattttct ttaaatgctt ttttaagttc
    17581 aatagtcttt ttacatttaa gcatagttct atcatcacta aataaaagaa tttgggataa
    17641 tgcttgtaaa tcatttcctt tataaaaacc attttcattt agaaattcat aacatttttc
    17701 tatgtattct aaatcttcat ctaaattttt tgaatgaatt gcaataagtg ctatattaca
    17761 atagtcatca ctagaagtta gaaatggatg ttttttcctc atgtaatcat atattatttt
    17821 catttttgat atacatgtaa gataatccat tttctctcta ttttcataaa ctattgatgc
    17881 tgtaagtggt aaatatgtat cgccccaaaa cttttcactt ttaagttctt tgtatatatc
    17941 taaaatcatc ttaaaactac tttctggatt tgattcaaac gaaagtagtg ttgccatata
    18001 aaacatatta tatcctctaa aattagagaa taatccagta ttttttttaa taagctctat
    18061 tcgttcttcc actaatctag gacttattct cttgttctta agagtaaatg caagtgcaca
    18121 actatgttgt atcaatccca tttgccaaga acctttaact tcatttaatg ctttgtaatt
    18181 ttctattaat aagtttactt ttccatttat aaacgattgt tccatattgc cctcctagta
    18241 atatatctca ttgtactaat agttttatat ttattatata tatattaaat actacaatca
    18301 atacatatta cttcaaagaa catcattctt caacaataat gtagcctttt ttgtaatgtc
    18361 ttcccatatt attgactcta tcatagtact ctgattcact ttcattactc catttgggca
    18421 caaatacatc tattgcatta gaattactaa aatacttact agacccccta tccataacag
    18481 tttttaattt tccatctaca ataatcttag ttccaaaact caagtgattg tttgctacaa
    18541 aaccatcttc taaatcatct cctgtagcag ttatcgctcc atatccattt tcttgattaa
    18601 gacttgtgta ataactaact tcaaaagtta taggtactaa cttctttatt ttaataccta
    18661 actgattttg tagtttctcc ttttgtactt tcaacttata ctcatcacta tttttataat
    18721 cttccatacc ttgattatac ttggaattaa agcttttacc tgactttact atagtatcta
    18781 tatttactat agattcttta caatgatctg catctatttt tgtttctcca tttaccaata
    18841 atggagttaa acatacacta catacaacac atgtaatcaa tgcttcttta agcttcataa
    18901 attcccccct tattattttg atatattagc atatttaata ttatatgtct atatttagat
    18961 ttaaatacca cacccatttg ctttaagaag ttgtcttata tttaattcac cttaaacact
    19021 tataaatcct ctatcttaga atcatataga ataaagcttt tatcgttaaa tattttttat
    19081 taatctttta aaatctaaag ctactttatt taattttctc ttttttagaa tttaagaatt
    19141 aaaattctat aattttaaaa catattgaat ttattttttt gagatgatat aa
//
LOCUS       DAGQWR010000082        18211 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.082, whole genome shotgun sequence.
ACCESSION   DAGQWR010000082 DAGQWR010000000
VERSION     DAGQWR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18211
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.082"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(236..1333)
                     /locus_tag="MIW95_002431"
     CDS             complement(236..1333)
                     /locus_tag="MIW95_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBY3151752.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILFTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            1626..3152
                     /gene="glpK"
                     /locus_tag="MIW95_002432"
     CDS             1626..3152
                     /gene="glpK"
                     /locus_tag="MIW95_002432"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBY3151753.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFESGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKEIKMLRVDGGITKNNFTMQFQSDILDIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTKQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            3193..4308
                     /locus_tag="MIW95_002433"
     CDS             3193..4308
                     /locus_tag="MIW95_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBY3151754.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(4589..5353)
                     /locus_tag="MIW95_002434"
     CDS             complement(4589..5353)
                     /locus_tag="MIW95_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBY3151755.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            5612..5702
                     /locus_tag="MIW95_002435"
     tRNA            5612..5702
                     /locus_tag="MIW95_002435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5646..5648,aa:Ser,seq:gct)
     gene            5790..6578
                     /locus_tag="MIW95_002436"
     CDS             5790..6578
                     /locus_tag="MIW95_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBY3151756.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHIGVLFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSVDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            6599..7339
                     /locus_tag="MIW95_002437"
     CDS             6599..7339
                     /locus_tag="MIW95_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBY3151757.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDSLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAVVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            7517..7891
                     /locus_tag="MIW95_002438"
     CDS             7517..7891
                     /locus_tag="MIW95_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151758.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKDNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            7983..9443
                     /locus_tag="MIW95_002439"
     CDS             7983..9443
                     /locus_tag="MIW95_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBY3151759.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTIGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIEEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLNVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFETWFESFKAELENAIKK"
     gene            9496..12372
                     /locus_tag="MIW95_002440"
     CDS             9496..12372
                     /locus_tag="MIW95_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY3151760.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKILNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILNDKNINDKPIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRIEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKDKKLKWIRMNIFEDELKVLG
                     VVVDVTKEFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLD
                     NLKYINDTYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRD
                     IINSVKKQMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTI
                     KGEVSEFDINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPK
                     IKTFSSPIDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLS
                     KVDFEEIEDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNG
                     DAVLLYIMPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVISEGVETKGEME
                     TLINLGVDYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            12449..14515
                     /locus_tag="MIW95_002441"
     CDS             12449..14515
                     /locus_tag="MIW95_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBY3151761.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVYTANRLIDKNKNRYEKVIKCSQGDGGSVTYISPHDSEEEAL
                     YIAKEIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    15107..15317
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15543..16358
                     /locus_tag="MIW95_002442"
     CDS             15543..16358
                     /locus_tag="MIW95_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBY3151762.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            16386..17063
                     /locus_tag="MIW95_002443"
     CDS             16386..17063
                     /locus_tag="MIW95_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBY3151763.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            17122..17844
                     /locus_tag="MIW95_002444"
     CDS             17122..17844
                     /locus_tag="MIW95_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBY3151764.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         7163 a         1929 c         3240 g         5879 t
ORIGIN      
        1 cgattgcgtc aaaaataaaa aacaattaaa tgtaagaata attatgtcaa aaatataaaa
       61 taaattaaaa gaaaaagatt atactgaagg aaaaagattt gaaatagggg tgaactttac
      121 atattatagt tcacccttat acaaaaatat aaaatataat ttattatagg aaaatttaaa
      181 aaaatcagtc tatttaattt atactgatgt attgtaggat tttacaaaca aactgttata
      241 taactttttt tatctccaat tttgatttta agatatctaa gaatttataa caatcttctg
      301 gatatatttc tcccatattt gcaacttgga atatgttagc atctaggaat ttgccttttc
      361 ctggatagac aacatagcca ctttcccttt ctaggcctaa tataaactca tttacattga
      421 tttctttagg tagaaataca gatgttactg tattagacat atacttatct ggaagtaaaa
      481 atgttagccc caattcttcc atgccattcc ttaaaatagc agaacattct ttgtatcttt
      541 ctattttact gtcatcttcc aaaaactcag ttaaagcttc attaagggca aatattaaag
      601 taacattagg tgtatttggt gtttgagaag atgatttagc aactttatag tgtttataaa
      661 gattaagata tacattctta cattgatttt ctttaatatt ttgtaaaata ctctctttag
      721 cacaaaggta tgcagaacct ggaaatgctc caagagcttt accaccaaca cctgttatta
      781 tatctatatt aaactcttct acatcaatat attcacctgc tgaggcactt attgcatcta
      841 caaaaaatat tttgttatac ttcttagcaa gttttcctag ttcagggact ggatttatca
      901 ttcctgaact agtttcatgg aacaccatag caattacttt tatatcagaa ttttcaatta
      961 tatatttttc taaaatgtcc aggtctgggt attctgccca ttcaaaatta ggcttatatg
     1021 tttttacatt ataagtaaat aatatttctt ctagcctttc tccaaaaaca ccattactaa
     1081 ctagcagaac tgcatcttct ttgtcaaaga tagaagatag tacacattca tttacagatg
     1141 ttccagagcc agatacaatt aatgagtagt aattagaagt tgcattatat agtttattga
     1201 ttttttgttg tgtatctttg aataaatcca taaactcgtt tcctctatgg catatatcat
     1261 agtgaagaag tgaatttctg actttatctg taaccattac tggtccagga ctgaatagat
     1321 atgttttttt cattgtaaag cctccttaaa aagttaaatt taaattttcg aatgactgac
     1381 tatgtgtaaa tattaaaata ttgtgtgtta ttttaactag attatagaat aaaataatga
     1441 atattgcaat aatatttcaa taaaaatact tttttaatca ccataattaa aaataacaaa
     1501 aaatattgta atgaatattg cataaatcca catgatgata taaaatataa ataacttgaa
     1561 tagattatat attcaaaaaa taaaaaagaa tttattaatt ctgaaaataa agggggacat
     1621 tttaagtgaa atatgtatta gcaattgacc aaggaacaac aggaacaaga gcagtattga
     1681 taaacgaaaa atcagagttt gtttttagcg attatatgga acatactcaa atatatccac
     1741 aatctggata tgttgaacat gaccctatag aaatttggaa taatacaaaa actgtgagta
     1801 ataatgtatt acaacaggct tttgaaagtg gaatcaatga aagtgatata aaaggaattg
     1861 gtatagataa tcaaggtgaa acagtaatgt tatgggataa aaatacaggt aaaccacttt
     1921 acaatgctat cgtatggcag tgtaggagaa cttatgatta tgtggaaaat ttaaagaata
     1981 taccaggttt agaaaataag ataatagata agactgggtt aattatagac ccatatttct
     2041 caggtacaaa aattaaatgg gtaatagata atgtaaaagg tgtaaaagaa aaaattaaaa
     2101 atggagaagt acttgcagga acattggata gttggttaat atggaaaatg acaggaggaa
     2161 aatctttttt aactgatgta tctacagcag caagaacaat gctttttaat ataagagata
     2221 tggattggga tagagagttg cttgacatta taggagtgcc tagaagtatt ttagcagata
     2281 taatgcctac tagtggtgat tttggaacta cagataagga ggcattttgt ggggtttcta
     2341 taaaaattac tgcttcaatg gtagaccaac cagcagcttt atttggcaat ggatgttttg
     2401 aaccaggaat ggttaaaaat acttatggta caggatgttt cttatatatg aatataggtg
     2461 aggagttaaa tatagcaggt aatggtattt tgacaacagt tgcttggaag ataggagata
     2521 aagttacata tgcttatgat ggtggagtgt atatagcagg tgctgcaata caatggctta
     2581 gagatggaat tggagttata aaaaattatt cagaaactga tgacatggca aattccatat
     2641 caagtactgg aggggtatat tttgtaccag catttgcagg tatagcagca ccttattggg
     2701 accaatatgc tagaggcaca atggttggga taacaggaag tactaaaaaa gaacatatag
     2761 taagggctac tcttgagtct gttgcacttc aagtcaagga tgttgttgat tccatgaatt
     2821 tggcttcagg gaaagaaata aaaatgttga gagtagatgg tggaattact aaaaataact
     2881 ttacaatgca atttcaatct gacatattag atataccaat tgaaattgca gaaaatcctg
     2941 aaacgacagt acttggtgta gggtatatgg cagggctttc ctgtggtgtt tggaacagta
     3001 ttgatgaaat tagaagtgaa tttacaataa gcaaaaaata tattccaaat atgactaagc
     3061 aaaaaagaga agaaatatta cttggatgga atcaagcagt aaaaagagct atgaattggg
     3121 aaaacgatac caaaaaggtg tgtgctaatt aagttacaat agagtatttt acatgtatag
     3181 gaggaattaa aaatgccctt tgataaaatt atattataca taatggctgt gggagtgctt
     3241 ttaggagcgt tagatagaat aattggaaat aaatttggac ttggagagca gtttgaagaa
     3301 gggtttaatt caatgggtcc attggcttta ggaatggttg gtatagtttg tttagctcca
     3361 gtaatttcag atgtactagg tcctataata ataccaatat tcaatctctg tggagctgac
     3421 ccatctatgt ttgcaactat tttagcgaat gatatgggag ggtatccatt ggctatggaa
     3481 cttgcacaca ataaagaggc aggaatgctt gcaggattag tcgtgtcatc catgcttgga
     3541 tgtacattag tattttcaat tccagtagga ctaggtctta tagaatatga agacagacca
     3601 ttctttgcaa aagggttact gataggcttg ataacaatac cttttggagg tattatagga
     3661 ggattaatag caggatttaa cttatcaatg gtaattataa atatgatacc agtgataata
     3721 ttggcagtaa tattggcagt aggtttaaaa tttaattcta cacttatgat aaatgggtca
     3781 ttagtttttg gaaaatttat atctatattg ataacgatag gtttgggagc agcagcattt
     3841 caagaaataa caggtatagt acttataaaa ggcatgacac ctataagaga aggtattcaa
     3901 gtaattggaa gtattgcaat agtgttatta ggtacttttc caatattaca tttattagtt
     3961 aaagttctta ataaaccact tacaatagtg ggtggaaaat taggtatgga tgccactagt
     4021 gcagcaggat tagtttttac tttagccaat agtattccag tatataaaat gatgaaagat
     4081 atgagtccaa aggggaaagt agtaaatact gcttggctgg tttgtgctac agcagctttg
     4141 ggggaccatt taggatttac agcaggagta gttccagaga tgattacacc tgttgtaatt
     4201 ggaaaattgg ttggaggagt attggcaatt gcacttgcta tacttatgac aaaagattta
     4261 actttggaag aagaacaaag tattaaaatg aagaaagatg cagtgtagaa taaacaatac
     4321 ttaagtcaaa tttaagttat tttaaagtag gagtttaaat aactaaaaat aaattaaaaa
     4381 gacagacaat taaaaaataa tcgaataacc aaaactgata aattaaataa aatggcaatc
     4441 aaataactaa aaatgataag ctaactgaaa gtagttacat aattaaaata taaagtatag
     4501 tttagacata agtggagcta aataaataat tatttggctc cttaatttta atttattaca
     4561 tcaaggaact aaaaatcaat catacaagct aattatcagc tatgactaaa ggtattcctt
     4621 tttgagaaaa ctctattatt atattatggt ctatactagc agaggtaata atagtatcta
     4681 tgtgttctat aggagctact gatgcaaatg aatttatacc aaacttagga ggttcgacaa
     4741 ctactataga ttttctagac ctttcaatca ttttttttct aaccagtgct tcttctaagt
     4801 gatagtcaga gattccattt tcaagagtga tacctccagc acctaaaata gctttatcaa
     4861 cattaaattg ttcaatagct gagagtgtaa gtggaccaga aaaagaacct tcagtttctc
     4921 taagatagcc accaagcaca tatactttta tattagtatt cataagttca tttgcaacta
     4981 aaattgagtt ggttattaca gttagattct ttttatcttt caaatttttt gaaacttcaa
     5041 gtacagtagt tccagtatcc ataaaaatag tatcatttgt atttataaac tcactacatt
     5101 taatagcaat ctgtttttta tcgtttgagt ttattttttt tcttttaaca tagttaagtt
     5161 ctacgctact tgctataact ggtatagcac caccataaac tctttttaaa agaccgatac
     5221 tttccatatg ggctaaatct cttcgtgcag tttctattga tatatcaaac atttgaacta
     5281 tgtcatttat tttaattata ttatctgatt tgagaatact taaaatcttc tcgtatcttt
     5341 cctgtggtaa cataaaatat cctccttgga ctagtattat tttagttttg gcataactta
     5401 caattaagtc agagtttagg tatatttgtt aatgtatgta tatttatcaa ataatattat
     5461 aagtgttatg caagataatt ttaccatatt taaaacttac atttaagcaa aaaagtaaaa
     5521 atagatgttt aataattcaa aataactcaa attttataaa acaattgact aaaatatgat
     5581 tttattatat aatttactag tatagagata tggagaagta ctcaagtggc tcaagaggat
     5641 cccctgctaa gggattaggc tgggtaaccg gtgcgagggt tcgaatccct ccttctccgc
     5701 caattgttaa aggctatttt tattaaagat agtcttttta taatgtcata aatgattaga
     5761 tatgtataat taaagaaagg agggaataga tgacaacttt aatttttttc atagtgggga
     5821 tagcatcttt ggtagttgct tttgttattg atgggggaca tattggagta ttatttgtat
     5881 tttcatcagc acttgttgtg tttggtggaa ctataggcgc tgttggagct tcgactccat
     5941 tccctgtgtt taaaaagtct ataaagataa tgttattagc ttttaaaaat aaaaagccta
     6001 ataacataga aaatataatc tatttcaaag aaattgcaac aaaggctagg aaagaggggc
     6061 ttcttagtgt tgacaaagaa ataagtgaga atgatgaatt agaccctttt attagaaaag
     6121 ggcttgaact tatggtagat ggggtggatg cagttactat aagaaatata ttggaatcac
     6181 agatacagat aacatcaaaa cgacataaag aaggaatagc aatttttgat tcagcaggtg
     6241 gatatgcacc tacactgggg ataataggta cagttatggg attagtacat attttaggta
     6301 atctttcaag taatccagat gcattaggac ctcaaatttc tgttgcattt ttggctactt
     6361 tgtatggtgt tggttctgcc aatttaatat ggttaccaat agcatcaaaa cttaaggcta
     6421 taaatcaaca ggaaataagt gaaagacttt tggtgataga agcaatatta tccatacttg
     6481 agggtgataa ttctaattta atgacagaaa aattaaaaat atttttaaat tcaaaagaac
     6541 taattgaact tgaagaaatt ggtggtagag ctgaataata taaaaggatg gaatgtttat
     6601 gttgtatgat gaagatgaaa aagaagaaaa caatgagcga tggcttttaa cttattcaga
     6661 cctcataaca ttacttatga ttttctttgt aataatgtat tctatgagta atgttgatgc
     6721 ggagaaatat aaacaattat ctcaatctct aaattctgct tttggtggtt cttctggagt
     6781 tattgaaggt ggtaatagca aaatagaacc agtagttgaa ccaggaagta atgatttaga
     6841 tagtttacaa aatgctaagt ttaaaaaagt tggtgaagaa atccagaagt atttaaatga
     6901 aaatggtatg gctaattcag tatcattaag agttcaagat agaggattag taataagcct
     6961 taaagataca atactatttg atacaggaaa agcagttgta aaggataatt ctagagataa
     7021 gattattcaa ataggtaaga tgttaaatga aatgaatagt tacatgagag tagaaggtca
     7081 tacagacaat atgtctataa aaaattcaga gtttaagtca aattgggatt tatcagttat
     7141 gagagcaacc aatgttgttc aacttcttat tgacaatgca ggtatagcac cagataaatt
     7201 atctgctgta ggatatggtg agtttagacc aatagcagaa aattcatctg aagaaggtag
     7261 gtcaaaaaat agaagagttg atatagtttt agttgatagt aaatatgata atgtagaaaa
     7321 tgtttcaaag gataaatagt taaattagct gtctcaatac agaattaatt ctaaaggaga
     7381 tggctttttt atgcgaaaat taaaaataga tacatagtaa gttacctttt tgtaggaata
     7441 tatggtatct taaatatata atatttaaat aatatataag ctcaattaaa aaaattttat
     7501 agaggatggg aaataaatgg ggaataaaaa taaaaatacg aaatataaag ataaaacaag
     7561 agaaagatat aataaaaaag ttgagaattt aaataaagaa aaattaaata aagataataa
     7621 acagtctaag aaggtagata agattgatga tattattaat aaatctagta ttgtattatg
     7681 tgtacttcta gtggtgtttt cattacttta cttatttaaa ttggttcaac ttaatataat
     7741 aatgatactt ttagtaatta cagtagctat tcaacaagga ttggtgtcat ttggtgcatt
     7801 tagaaataaa aattatgtaa aagctttatt ttctatattt gtaatagtat gtttcttaat
     7861 tgcttttaaa tatgttgtaa atcctgttta aatatatcaa taaacgttct ataattaata
     7921 taaatagtga agttacagtt ttaatgctat tataaatttt ataatttgaa ggagtagatt
     7981 ttatgaatag gtgtaagttt gcaaaattaa ttatcacatc aatgattgtt ttattactta
     8041 caggatgtag tttaaaaaag gaagataatt ataagttaga cccagaaaat ccgacatcaa
     8101 tacaaatttg gcattattat aatggacagc aaaaaatggc ttttgataag cttgttaagg
     8161 aatttaatga cactataggt gctgaaaaag gaattattgt tgaagcgttt agtcaaggta
     8221 atgtagttga tttatcagag aaagttttag attctgcaaa taaaaaagtt ggctctgaag
     8281 atgtgccaaa tatatttgca gcatactcag atactgcata tgaaatggat aaattagggt
     8341 tactagtaga tatggataag tacataagta aagaagaatt aaatgaatac atagacgaat
     8401 atgtagaaga aggcagaata aattctaagg atgagtttaa acttttccca attgcaaaat
     8461 caactgaaat tttgatgtta aataaaactg attgggataa atttgcaaaa gctacaaatt
     8521 caaatataaa tgatataaaa acaattgaag gattagtaga gacatcaaaa aaatattatg
     8581 aatggacaga tagtttaacg aaagagccta atgatggtaa agcatttttt gggagagatg
     8641 ctttagcaaa ttatattatt ataggaagta aacaactagg agtagaaatt ttttctgtta
     8701 aagatggtaa agttacacta aatattgaag aaaaagttat gaaaaagcta tgggataatt
     8761 tctatgtacc atatataaat ggatattttg cttcttatgg taagtttcgt tctgatgatg
     8821 caaaaacagg tgatatatta gctcttgtag gctctagttc aggagcagca tactttccag
     8881 atagagtaat gctaaatgat aatgagagtt ataatattga aacatctgtt tttcaagcac
     8941 cacgttttaa aggagaagga aaatatgctg tacaacaagg tgcaggaatg gctgtaacaa
     9001 aatctactaa agaagaagaa tatgcatcag tagaattttt aaaatggttt acagataaag
     9061 agcctaatat aaaattctct attgcttctg gatatttacc agtaaagaaa aaaacgaatg
     9121 atttgaatgt tataaaagaa gttatgaaga aagataattt gagtttatct gataaagttg
     9181 aaggttcaat agcctcatct attgaacaaa ttaacagttg taatttatac actaataaag
     9241 catttgatgg tggaactaaa gctagacaaa tacttgaata ttcaatgctt gataaagcta
     9301 aagaagattc aaataaaata aatgaattgg taaaaagtgg aacttctaag aaggatgcgg
     9361 tagcacaatt taataatgat aagaattttg aaacttggtt tgaaagtttt aaggctgagc
     9421 ttgaaaatgc tataaaaaaa tagatgaatt ggcagttagt gtgtagacaa gcacaaaggt
     9481 ataggtggaa taagtatgga aaacaatcta agaaaaaaca attcaataat gaaaaagatg
     9541 atatttcccc taattattgt tatgctaatt caaacaagtt tattttgtgc cacaatttta
     9601 tggggaggaa ctattaagaa attaaatgat aattcgtttg acatcttaaa tgaaagagta
     9661 attaatcgta aaaattatat acaaaatgaa atgttacaaa gatggtcaaa tacaagtgaa
     9721 acggaagcta caattaattc ctctgtaaaa aaaatattaa atgaaaaaaa tgccttaatt
     9781 gaggatgtag gcaacaatga agaaattaac aaagatatta taaaaagcat atcaaatgat
     9841 ttaatatatc ttttgagaaa aaactctgtt acaggagctt ttataattct aaatgacaaa
     9901 aatattaacg ataagccaat agatagtaaa ggtgaaataa gtaggactgg attgtatata
     9961 agggatttag accctaagtt taatccaaat gataattctg atttgttaat tgagagaggt
    10021 tcatcagata tagcaagaag ttttggaata tctatggaca gttattggga acctaaattt
    10081 tcatttaaaa gtgaaaatga gcaagaggga gatgattttt tttataaacc atttagagct
    10141 gcaattgata atccaaacgt atcaagttct aatttgggat actggggaag taagttttta
    10201 ttgacaaatg caggtgatag gcttatgaca tatacagttc cccttattta tgaagatgga
    10261 actgtatatg gagtacttgg aatagattta agtgtaaatt atttaagaga attacttcca
    10321 tactcagaga taaatggaaa taaaaaaggt aattatattt taggtgtaga tagaaataat
    10381 gatatggcct ttgaaaatgt aatttcaaat ggatactcat taaaagaaaa attttgggat
    10441 ggtttacatt ctaatttaga gcagaaggaa tcgtataaaa atatatataa gattaaaaat
    10501 aaagataatg aatgtggtaa tggcaacatt tatgctagtg tacaatactt agatttatat
    10561 aactccaata ctccatttga aaatgaacgt tgggctcttt tgggtattat tgaagaaaaa
    10621 agtcttttgt cattttcaaa aaggatagag aaattagtta taatatctat agcaatttct
    10681 ttattaatag gtctaatagg tatttacatt gcatcaagat tatttacaaa accaattgtt
    10741 cttttagcca acaaagttag aaacagtaat ccatcaaaac cagtaaatct tgataagata
    10801 aatatatctg aaatagatga attatcttca gctatagagt tactcagtgc aaatgttgca
    10861 gattcttcat caaaattatc acagattatt gaaatggtag atatgccaat cggggctttt
    10921 gagttataca aaaacgcaga gaatgttttt tgtacaaaag ataaaaaact taagtggata
    10981 agaatgaata tctttgaaga tgagctaaag gttcttggtg ttgttgttga tgtcacaaaa
    11041 gaatttattg agaaaaggaa gatagaatat gaaagagatt ttgatatttt aacgaatctt
    11101 ttaaatagga gagcttttca ttcacagata caagaaaaat ttaagaataa agatgaactt
    11161 aagatagcag catttattat gtgggattta gataatctaa aatatattaa tgatacatat
    11221 ggacatgatg ctggtgatga atatattagg tgtgctgcag acatacttaa gaagtttata
    11281 tcatggaatg gtatagtatc tagactttca ggtgatgagt tttatgtatt tatatatgga
    11341 tatgaaagta aagatagtat aagagatatt attaattctg ttaagaaaca gatgaataat
    11401 accttattaa aacttcatga tggaacagaa tttaaaataa gggcatcagc aggaatttcc
    11461 tggtaccctg atgattcaac caactatgat gaattaatga gatacgcaga ttttgctatg
    11521 tatcaaatta aaaatacaat caagggggaa gttagtgaat ttgatattaa tatatataat
    11581 aaggattcat tcttattgag taatagagaa gaattaaaca agttaattga tggtcaatta
    11641 gttgagtatg catttcagcc tattgtagat tgtaaaggta aagtatttgc atatgaggca
    11701 cttatgagac ctaaaataaa aacttttagt tcaccaatag atgtaattag attagctcgt
    11761 tcacagtcta aattatatca aattgagcgt attacatggt ttaaagcact taaatcattt
    11821 aaaaaataca aagaaaaatt tggcgattgc aaactgttct taaattctat tcctaataat
    11881 aatttatcaa aagtagattt tgaggaaatt gaagatttat atggtgaata tttaaataga
    11941 gtagtattag aaataacaga gaatgataag attaatgaag attttattga gaaaaagaaa
    12001 gcatatatag accattggaa tatagaaatt gctttagatg actttgggac aggttacaac
    12061 ggagatgctg tactattata tataatgcca gactatgtaa agattgatat gtctattgta
    12121 agaggtattg ataaagatga aaacagacag aaaatattaa aaaatcttat ctcatattct
    12181 aacgaaagaa atattaaagt gatatcagaa ggtgtagaaa caaaaggtga aatggaaaca
    12241 ctgattaatc ttggagtaga ttatatgcag ggttactata ttagtaaacc atcatttata
    12301 cctgaagata taagtgacaa aattaaaaaa gagctcaaga tatgtcatga aaataggaat
    12361 gatgtggttt aatagaggaa aaattaaatt tatttttatg aacttttagt ttataattaa
    12421 aaagaaaata atataattat aaattaaggt ggtggagcta ataatcacaa caaacataga
    12481 tataagaaag ttaaatgaaa atcaattaga agcagttgaa catatagatg ggccttgcat
    12541 gatacttgca gggcctggtt caggtaagac cagagtaatt acatatagaa tagccaatat
    12601 ggttgtaaac aaaaatatag caccaacaag aatcttggca ataagtttta ccaaggcatc
    12661 ttcaatagaa atgaaaaata gagctttgaa tttaagtgat gatataagac ttaataaggt
    12721 aacctatggt acttttcact ctgtattttt taaaattcta agatactttg aaagatacaa
    12781 tttagacagt atatttgatg aaaagtcaaa gcgaatgaca atcaaggcaa tcctaaaaag
    12841 tttaaatata gagaatgctg atgatgatga gaatatagga caagttataa atgaaatctc
    12901 ctatgtaaaa aatgaactta tggataaaaa tgaatttaat tcagaagtat taacaaaaga
    12961 tgagttttta aaagtataca acttatacga agaacaaaaa tctaaagtta acaaaataga
    13021 ttttgatgat atgcttataa gaacttatta tttacttttg aacaataagt ctgcactaga
    13081 aatggttaga aatgtgtata aatatatatt gatagatgaa tttcaagata taaataaagt
    13141 acagtttgaa gttttaaagt taatttgtag cccattaaac aatatatttg cagttggaga
    13201 tgaagaccaa agtatatatg gatttagagg tgctagacca gattttttgc ttgaatttga
    13261 acaatacttt aataatacta aaaaaataat attagatata aattataggt ctaaaagtga
    13321 gatagtgtat actgcaaata gacttatcga caaaaataaa aatagatatg aaaaagtcat
    13381 taagtgtagt cagggggatg gaggaagtgt tacctacatt tctccacatg attcagagga
    13441 agaagcctta tatattgcca aggaaataat tgacgaaata caaaaagatt atgtagagta
    13501 ttcagatttt gcagtaattt atagaacaaa tatacagtca agagcattag tagatgtatt
    13561 tatggatatg aggattccat ttgtagtaaa agactcagta attacaatat atgaccattg
    13621 ggcaagtcaa gatatactag cttatttaag aataggtatt aatccaaaat caaataaaga
    13681 ttggttgaga attataaaca agccttttag atatatatcc aaagacagta taaatatggt
    13741 taaggatgaa aaagacttta taacagcatt aataaataag tgtaatcttc atcctaaaca
    13801 agttaaaaca ataaatgatt tggaaataga tttaagttat ttaaatactt taaatcctaa
    13861 aaatgcaata tcttatatta gaactagtct tgattatgat agatacgttt tagattattg
    13921 tagcaacaga aagataaaaa ctaatggact tgtagagata ttaaatgaac ttgagagttc
    13981 agcaactaat tttaatactg ttacagagtt tttagagcat attgatagag taaagactga
    14041 gttaacggaa aataacaaga ataagcagac tgagggtgtc atatttacaa ctatgcatag
    14101 tgcaaaagga ttagaattta gaaatgttta tattataggt gtaaatgaag gaacaatacc
    14161 tcatgaaaaa tcatatgata tttgtaagga agagaaaaaa gaagaacaat tagaagaaga
    14221 aagaagactt atgtatgttg ctattacaag agctgaagaa aagttatgta taagctctac
    14281 tttaaataag tatggtaaga aagttgataa atcattattt attaatgata taaaatctcc
    14341 aacgaaaaaa gaaattgaca gtattggtgt aggggataag gtataccata aaaaatttca
    14401 tgaaggagaa ataattaaaa aagatggaat tatgtttaca ataaagttta aagatagaga
    14461 gcgaatcttg gatttgaaaa cttgtttatt gaagaatata atttatacta tttaatttag
    14521 aatctaaatt tagatagaaa tataatgtat tatttattat gtgcaagaca aatagatatg
    14581 tgtaaaatgt attatttaac ttattagaaa aagaaataag gatttaatat attatttata
    14641 gttgtttaga aaaagcaaca atgtggaaaa tattatttag tattattaaa gaaaagcaac
    14701 aatgtgaaag atattattta gtgtaattta agaaaaagca acaatgtgaa agatattatt
    14761 tagtattatt aaagaaagag caacagtgtg aaagatacta tttagtatta ttaaagaaaa
    14821 agcaacagtg tgaaagatat tatttagtgt aatttaagaa aagcaacaat gtgaaagata
    14881 ttatttagta ttgtttaaga aaaagcaaca atgtgaaaga tattatttag tattatttaa
    14941 gaaatagcta aaaatatgaa atatactatt tataattaca aaaataatat caataaatat
    15001 aaacagtagt atttagataa tttacaagta aaaattatta aaaaaataaa aaacttattg
    15061 acaaaaatat tataaggaag tacaattgta ttaacaattt aaggaaacaa atccaatgat
    15121 agagaaagta gcatatatgg atttacagcg agttaggggt tggtgtaagc ctagcaatga
    15181 agtctatgtg aagagagctc tggaggagat tatcagaaac tttagtatga taattggttt
    15241 acccgcctta agggattgag aggataagta cttacttatc aaaaagagtg gtaacgcgga
    15301 tataattcgt ctcttagctg taaagctaag ggactttttt gatttaaaaa taaaaatatc
    15361 catacagctt aaatgctaac agtcacaaat tttgaaagta atataagtat ttatcaataa
    15421 gagtggtaac gcggtaaagt cgtctcttag tcattaaaga ctgagagact ttttttgtaa
    15481 ctcgaaacat ttaagtatta aaatctgagg gaaattataa ttaaaagaga ggggtagtta
    15541 aaatgaaagt atttaaaaaa ttattaagtt taggattagt attaggattg acattatcgc
    15601 tagtaggatg tagtggtgga ggagaaaaaa caaagttaga acaaataaaa gacaatggaa
    15661 aattagttgt tggtactagt gcagaatttc ctccatttga atttcataag gtagtagatg
    15721 gaaaagattc aataaaagga ttcgatataa tgttggctga agaatttgca aaagagcttg
    15781 gtgtaaaggt tgaaattaag gatatgtcat ttgatggatt aataggtgct ttaaatgcag
    15841 accaagttga tattgttctt gcaggtatgt ctccaacacc agagagagaa aagagtgttg
    15901 atttttcaga attatattat ttaagtagaa atgcagttat agttaaagat gcagatatag
    15961 acaaagtaaa aactgaagat gatttaaaga aacttagagt tggagttcaa gcaggaagta
    16021 tccaagaaga atatgtagtt aacactttaa aaatgacaac tacaaaatct ttaaaagcaa
    16081 tacctgattt aattacagaa ttaaaaaatg gaaacataga tgcagttgtt actaatgaag
    16141 cagtttcttt aataaatgtt aaaaaatatg atggaataaa aatggcaaat actgaagttg
    16201 gtaaagatgt aacagaaggt atggcagcag caatcaaaaa atctgacaat aacaaagatt
    16261 ttatagaatt attaaacaaa aagataaaag aattacaaga tggtaagaag atagaagaat
    16321 ttttaaatga agcatctact gaagctgctt caaattagta agaaagttat gtgggagatg
    16381 aaattatgag tttagatttt agttttttaa gtagatttgg gacttctttt ttggaaggaa
    16441 caggtgtgac agtatcaatt tctcttgtgg cattatgctt tggatttata ataggtataa
    16501 ttatctgtat ggcaaaaata tcaaagagca aagtattaag agcaatatca tcaatctata
    16561 ttgaggtttt aagagggaca ccattattag tacagatata tatagtatgg tttggtttac
    16621 ctcaattagg aataagattc cctatgttgt ttggtatacc aagtgaattt atagctagtg
    16681 catttgcatt atcagtaaac tctggtgcat acgtggcaga gatacttaga tcaggtatac
    16741 aatcagttga taatggacaa atggaagcaa gtagatcatt aggattaaat tattggtcaa
    16801 ctatgagata tataataata cctcaagcta taaaaaatat attaccttca ttagcaaatg
    16861 agtttattac acttgtaaaa gaatcttcaa taatatctgt aataggagtt gttgagataa
    16921 tgcgtactgc tgatatagta aaaaatgcag catttagagc attagagcca ttaatagtag
    16981 cagcagcaat ttactttgtt ataacattta ctctatcaag attagttgga ttattagaaa
    17041 agaaactatc tgtgtctaac tagatgagag catgtttagc atttagggaa aacatagaat
    17101 agtaaaaagg aaagtgataa tatgattaca ataaaaaatt taagtaaatc ttttggagat
    17161 ttaaatgtat taaaaaatat agacttagaa atcgctaaag gcgaaataat ggttatagta
    17221 ggtccaagtg gttctggtaa aagtacactt cttagatgta tgaatttact agaaatacct
    17281 acaggtggag aaataatatt tgaaggaaaa aatttggtcg ataaaaaaac taatattgat
    17341 gaggttagac aaaatatagg tatggtgttt caaaacttta atttattccc acataagact
    17401 attttagata atataacatt agcaccaatt aaattaaaaa agatgactaa ggaagaagca
    17461 gaaaaaaaag cagaaatttt gttgagtaga gttggtcttt tagataaaaa agattcatat
    17521 ccatcacaat tatctggagg acaaaagcaa agaatagcaa tagctagagc acttgctatg
    17581 gaaccagata tgatgttgtt tgatgagcca acatctgctc ttgacccaga aatggtaaac
    17641 gaagttttgg atgttataaa agaacttgct aaagaaggta tgacaatggc aattgtaact
    17701 catgagatgg gatttgctaa ggaagttgca gatagagtta tttttataga tggaggaagt
    17761 atacttgaag ataatactcc agaagaagtt ttcggaaatc caaaacatga aagaacaaaa
    17821 gcatttttag caaaagtgct ataatatata aaaaggatgt tattgatatt agaaattcaa
    17881 tacatccttt tttagtgatg gttaatatta attttaaaat taaagttata ttaatattaa
    17941 ctttattttt acgatttttt gcagaaaaaa aaagagctcg gggtaagctc tttttttcca
    18001 ccgaattaat ttccgttact aatccgattt ttaataaata taaaatatat ccttatgaaa
    18061 gggtattggg aatagatata ttaactaact agggttacca gggggataac cttaatttat
    18121 tataacaaaa tgcgacacaa aaatcaatat tttttttgaa ataaattata aaagttattt
    18181 ttataacaag taagcttata aatttaactt t
//
LOCUS       DAGQWR010000083        18145 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.083, whole genome shotgun sequence.
ACCESSION   DAGQWR010000083 DAGQWR010000000
VERSION     DAGQWR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18145
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.083"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            18..242
                     /locus_tag="MIW95_002445"
     CDS             18..242
                     /locus_tag="MIW95_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151765.1"
                     /translation="MLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQ
                     ILDYYQCYEINIEIKNEFKKLRFNSYVTRK"
     gene            226..942
                     /locus_tag="MIW95_002446"
     CDS             226..942
                     /locus_tag="MIW95_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151766.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVVEFTEEDEEYEEELKQNIIE
                     NIKKNFDEKKLVQVYKYRGV"
     gene            962..1381
                     /locus_tag="MIW95_002447"
     CDS             962..1381
                     /locus_tag="MIW95_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151767.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            1371..1895
                     /locus_tag="MIW95_002448"
     CDS             1371..1895
                     /locus_tag="MIW95_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151768.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYVDENNNEESISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVLITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            2207..2764
                     /gene="efp"
                     /locus_tag="MIW95_002449"
     CDS             2207..2764
                     /gene="efp"
                     /locus_tag="MIW95_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBY3151769.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            2856..3311
                     /locus_tag="MIW95_002450"
     CDS             2856..3311
                     /locus_tag="MIW95_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY3151770.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCPSCDDRE"
     gene            3474..4184
                     /locus_tag="MIW95_002451"
     CDS             3474..4184
                     /locus_tag="MIW95_002451"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBY3151771.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            4238..5308
                     /locus_tag="MIW95_002452"
     CDS             4238..5308
                     /locus_tag="MIW95_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY3151772.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            5336..8890
                     /gene="smc"
                     /locus_tag="MIW95_002453"
     CDS             5336..8890
                     /gene="smc"
                     /locus_tag="MIW95_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBY3151773.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKILEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKVSLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEAYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSIATEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIGSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            8917..10197
                     /gene="ftsY"
                     /locus_tag="MIW95_002454"
     CDS             8917..10197
                     /gene="ftsY"
                     /locus_tag="MIW95_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBY3151774.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDSLENLEETIFSG
                     LEEEVIDKVEDIEEKTEESVNNEGTEEIENFEKIEVEMDSNAKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            10300..10668
                     /locus_tag="MIW95_002455"
     CDS             10300..10668
                     /locus_tag="MIW95_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBY3151775.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            10665..12017
                     /gene="ffh"
                     /locus_tag="MIW95_002456"
     CDS             10665..12017
                     /gene="ffh"
                     /locus_tag="MIW95_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBY3151776.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMAELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            12055..12327
                     /gene="rpsP"
                     /locus_tag="MIW95_002457"
     CDS             12055..12327
                     /gene="rpsP"
                     /locus_tag="MIW95_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBY3151777.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            12392..12619
                     /locus_tag="MIW95_002458"
     CDS             12392..12619
                     /locus_tag="MIW95_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBY3151778.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            12727..13242
                     /gene="rimM"
                     /locus_tag="MIW95_002459"
     CDS             12727..13242
                     /gene="rimM"
                     /locus_tag="MIW95_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBY3151779.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            13251..13946
                     /gene="trmD"
                     /locus_tag="MIW95_002460"
     CDS             13251..13946
                     /gene="trmD"
                     /locus_tag="MIW95_002460"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBY3151780.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVYIYNIRDFSNN
                     KHKKVDDYPFGGGVGMVMTPQPIYDTYKHIITTHNISNPSVIYLTPKGKVYNQSIAKQ
                     MSLKEDIILLCGHYEGVDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            14131..14481
                     /gene="rplS"
                     /locus_tag="MIW95_002461"
     CDS             14131..14481
                     /gene="rplS"
                     /locus_tag="MIW95_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBY3151781.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            14802..15698
                     /gene="ylqF"
                     /locus_tag="MIW95_002462"
     CDS             14802..15698
                     /gene="ylqF"
                     /locus_tag="MIW95_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBY3151782.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINNIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIAATV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            16087..17379
                     /gene="brnQ"
                     /locus_tag="MIW95_002463"
     CDS             16087..17379
                     /gene="brnQ"
                     /locus_tag="MIW95_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBY3151783.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVDVPVV
                     KSLPLHSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         7788 a         1665 c         3353 g         5339 t
ORIGIN      
        1 atatatctga cagagaaatg ttaagtacag caaaaaaata tatagaagat gagaagtata
       61 gaatacaaaa tagtaagtat gagttaattg aagataagat agaaaaaaat tacataaatg
      121 gatatgaaat taacagtaga atagagcaaa ttttagatta ttatcaatgc tatgaaataa
      181 atatagagat aaaaaatgaa tttaaaaaac tgaggtttaa tagctatgtt actagaaaat
      241 aaaggctcta tattaataat aacattgata atattttcta ttataagtac tgtgtgcatt
      301 atgtgtatag gtttaattta tacacataat aatatgtttg agttaggata caaggacatt
      361 caactaaaag aaatttcttt aagtggaata gaaatatcag taagtaatgt actttcttgt
      421 gttgaggaag ctattgatgt atctaataat gaagacgaat ttagagaata tttcttagga
      481 aataatatga ttgaatgtat aaaaaaaata aaagatacat cttatagtag tttagaagaa
      541 ctttctttaa aagtagaaaa taatactatt tatgacagag gagatttttt tgaatttgat
      601 atagaaagta atgttagaga aaaagaattt ttaaaagcaa taaaagtaag agtgaaaatc
      661 aaaaatccat ggataggaat tgacattgaa gattatatgg ataaatctaa taacaataaa
      721 attgatgaca aaacattcaa taattataaa gcatctgaaa ataatgaaat tctaaatcaa
      781 tttaaaaatg aaagtgaaaa tataaggaat caagaggttg ttgaatttac agaagaagat
      841 gaagaatatg aagaagaact taaacaaaac ataattgaaa atataaagaa gaattttgat
      901 gaaaaaaaac tagtacaggt atataagtat aggggtgtat aactttgaaa aaagaaggaa
      961 tgtgactgta atagaattaa tagtatctat cagtataata ctcttgatag ttacattatc
     1021 ttttccaaaa gataatcttg aaaatcataa aataaattta tttgcacgtc aactttgttc
     1081 agatataagg tatgttaggc gaattaatat gtttagcaac tacaatacat atatatatta
     1141 tataaatcaa ggtaaacacg aaggttatgt attgagaaaa gatggtaaaa acattaaatc
     1201 aataatgcta cctaaaaatg caaaaatagc tagaagtatt gaaataataa agtttaaatc
     1261 tgatgggtcg cctcaaaagg cgtctacaat agagatatat aataatagat taagaaaaac
     1321 tataacaata acacctgtaa gtggaagagt acttctgaaa gagggaaaat atgaaacata
     1381 aatatggtta cttgcttttg gaaagtgtag tatcattgtc ttcaatggtg ataattatac
     1441 tagttttata ttctatattt ttgtctacaa taaacttaaa gttaaaagta gaagataaga
     1501 tagaactaca acaacaaagt cttgaaatca taaaatctat ggaaggaatt attagcaatt
     1561 ctatgggaat aatgaatgta tctaattatg aagaaacatt taaaaagaca acgtctataa
     1621 aatgtagata tgtagatgaa aataataatg aagaaagcat aagtaataaa gaaattatat
     1681 tgaatgaaag acgaaataag ttatttgtaa attctttaaa tggcgaaagt agtcaagcag
     1741 gtggttatga aattggagat tatgtagatg agatgtatgt attaattact aataatggtc
     1801 aatatgttaa tattaaattg aaattatcaa aacgtagtca aaaatatgag actgatttca
     1861 aaataaaagt atggaatttt agtgaaagta tataaaaata cataaattta agataaagaa
     1921 atgacatcaa taagtcatat aaaatataat tattgagaac tatataacta aataatatca
     1981 atatgtaaag tgtatagatt aagaatatag aggataataa taaaaatata gaataagtaa
     2041 tttgccaaat acttgtttat attgtataac ttatgatatt atgatattag taaaaatttg
     2101 taatcctgac ttgacagaat tcaagtaaga gatataattt taaaggcata gtaatgtaaa
     2161 atttactaaa aaataagaaa tttattttaa tatggaggaa caaaaaatgg tatcagcagg
     2221 tgattttaga aaaggtgtta catttgaaaa agatggacaa ccatgtttag tagttgattt
     2281 tcaacacgtt aagccaggta aaggagctgc tttcgttaga actaaataca aaaacttaaa
     2341 aacaggagct ataagagaag aaagtttcaa tccaagtgaa aaattcccta aggctgttat
     2401 agatacaaga caaatgcaat atctatacaa tgatggtgag ttatattatt ttatggatca
     2461 agaaaatttt gaacaaatac cattaaacta tgagcaagtt gaagatgcaa ttaagttctt
     2521 aaaagaaaat gaagttgcta caataagatt ttaccaagga caaccattcc aagtggaagc
     2581 gccaaacttt gcagagctag aagttacaga tacagagcca ggtataaaag gtgatacagc
     2641 aagtaatgta actaaagcag ctacagttga aactggagca gttgttcaag ttccattatt
     2701 tataaatact ggagataaag taaagataga tactagaaca ggtgaatatt tatcaagagt
     2761 ataaaaattt atgtatataa aagcttatat gtcttaataa tataagatat atgagctttt
     2821 agtttatata taaaaaagaa agcgaggggt gttacatgaa acatttatat gaagaagttg
     2881 catacttaaa aggcctagca gaaggactag aaataagtgc tgaaagtaaa gaagggaaaa
     2941 tgatacataa aatagttgat gcattagaag tatttgcaga tgctattgta acattggatg
     3001 aagaacaaga agaacttcaa gattttgttg aatctataga tgaagattta gcagatttag
     3061 aagaagatat tgctgacatg gaagaagatc tttatgaaga agatgacgat gaggatgatg
     3121 aggacttcag ctatatagaa atggaatgcc caaattgtgg agagttagta gaaatagacg
     3181 aagatttgtt atatgatgat gaagtagatg ttgtttgtcc tgattgtaag gctgtgatat
     3241 tatcatcaga agatgattgt gatgatgatt gtacatgtgg tggttgtcca agttgtgatg
     3301 atagagagta aaaattcata agaattaaaa ctgcctatga tgcctatgag atattttgct
     3361 tcataggtag ttttattttt atgaggaagg gtattatgta ttttggtttt gcaatacttg
     3421 gacaaaaata gtacactaga atttatagaa taggattttg gagggatgta aatatgaaaa
     3481 taagtaaaaa attactagat aatatacaaa gatttgaaaa tgttataaat tataaattta
     3541 aaaataaaga atatatacta gaagctctta ctcatagttc atattctaat gaaaataaaa
     3601 aatataattt taacgaaagg ttggaatttt taggggattc ggttttaggc atagtgataa
     3661 gtgattattt atttaatgaa gaagctaatc tgcccgaagg ggaactaact aaacttagag
     3721 caaatatagt ttgtgaagat tcgttaagtg aagtagctaa tgatataaat ttaggtattc
     3781 atatgttatt aggtagagga gaagaagcaa caggaggaag acataggact tcaatattag
     3841 cagacgcatt tgaagctgta atagctgcta tatatcttga tggaggtttt gaaagtgcaa
     3901 gacagtttat acttcatcat atggaaaata taatttatga ttcaagaaaa ggaaatatat
     3961 ttagagacta caaaacgcat cttcaagaag tgttacaagg gaatggagaa aataatatct
     4021 ggtacaggct tatagaagaa aaaggaccag accataataa aagatttgta atggaagttg
     4081 gaattaatga cgatgtattg ggtataggag aaggaaagag taaaaaagaa gctgaacagt
     4141 tagctgctaa aatagcttta aagaaaaaat tatgggaaaa ataaatataa tatgtaaaaa
     4201 tataaaaaga aatatttata ttagaaggat gttgtagatg aaaaagagaa ttatacctat
     4261 atttgttcct cacaaaggat gtcctcatga ttgcatattt tgtaatcaaa aaaaaataac
     4321 aggtgtgagt actgatgtta ctagtgaaga tgcaagaaat ataattgagg aatgtttaga
     4381 gactatagat aaagatgcag atgtagaaat agcatttttt ggtggtagct ttactgctat
     4441 agatattgat atacaaaaaa gtttattatc agttgctaaa gaatatgtag aaaaaggtct
     4501 tgtaaaagat attagaatgt ctacaagacc agattgtatt gatgaagata tattaagtat
     4561 gctaaaagaa tataagacaa gtataattga actaggagta caatccttag atgaaaaagt
     4621 attattagat agtataagag gtcatcaatc tgaaattgta tacaagagtt caaagatgat
     4681 aaaaaatagt ggaatcaaac ttggattaca gatgatgatt ggtcttccag ctgacacaga
     4741 agaaaagtgt atatttacag caaaaaaatt tgtagagcta aaaccagatt gtgttagagt
     4801 ctatccaact ttggtagtaa aagatacagg tcttgaaaag ttaatggaac aaaataaata
     4861 taatccattt actcttgaag agagtgtaaa tatagtaaaa aaagttcttg tactgtttta
     4921 tgttaataat atcaatgtaa taagagttgg gcttcaagct actgatgata tacaaattgg
     4981 aaaagctgtt ttagcaggac catatcatcc tgcatttaga gagttggtag aagcagatat
     5041 gataaaagat tatttagaat ttgttatttt gcaaaataaa aatataaagc aaatgttggt
     5101 caaatctaat aaaaagaata tttctaaaat cataggaaat aaaaaaacaa atgttaaata
     5161 tatggaagaa aaatttggtg tgcttttaaa aacacaagaa tccgatttag atataaatca
     5221 attagaaatt gtgttagatg ggaaaagttt aattagtgct aatatgagag acattcatag
     5281 aaaattgtat gacatttatg acctttagtt ttaataatga agggagagaa aagatttgta
     5341 tttaaaaaga ctagagttaa agggatttaa atcctttcca gtaaaaacag atattatttt
     5401 taaagaagga ataacagcca tagtaggacc aaatgggagt ggaaaaagta atatatctga
     5461 tgctgtaaga tgggtattag gagaacagag tattaagagt cttagaggtg ataaactcga
     5521 agatgtaata tttgcaggta cagatacaaa aaaacctatg aattattgtg aagtggctct
     5581 tactattgat aattctgaaa atcaattgga attagatttt acagaagtaa ctataagaag
     5641 aagggcttat agaaatggtg aaagtgaatt ttttctaaat aataaaagtt gtagattaaa
     5701 agatataaaa gaagtctttt tagatactgg tattggaaaa gatggttatt caatcataga
     5761 acaaggaaaa gtagatgaaa tattaagtaa taatccttta agcagaagaa aagtttttga
     5821 tgaagcttgt ggcatatcaa aatatagata taaaaaacag gaagcagaaa ggaatttaag
     5881 taatacaaag gaaaatttag aaagaataga tgatgtatat atagaaattg aaaatcaatt
     5941 aaagcctctt tttaatcaac agacaaaggc aaaaaagtac ttggaaataa gtgaaaaatt
     6001 aaagatactt gaagtaaata gctttataag agaaattgaa ggaatagaaa aagaattaag
     6061 tgaggttaat gaacatcgta aggttattga aaaggagctc aatgaaaaag aagaacaaaa
     6121 aaatgttgta gagaaaaagc aagaagatat taataaagag gtagaggtat tacaagatgt
     6181 aattgaaaaa tctgtagatt atataaattc aattaaagga gttatatcaa aaaaagagtc
     6241 ccaaataaat ttaattaagg aaagaataag aaattttacg aatgaaatta gtagaaaaaa
     6301 tttagaaata aatgatataa aagagaagct aaatgaaaat aagcaataca taaaagaatt
     6361 agaaagtaat aaattatcag gtagtgaaga actatctacc ttgcaggaaa atataaaagt
     6421 attggaggca agtaaagata aacaaaaaat taagttagaa tccctaaata atgagataga
     6481 attattgaaa gaaagtataa ttgatatatt aaataagaaa caagagttta gtaataagct
     6541 gtccacttta aacgcaaata aagaaaatat gaatatcaga gatgaaaata taaattcaga
     6601 gataacagag ttaaacaaaa atattgaaat aaaatcatct gaattagata ctataaataa
     6661 agaatttaat atgcaaaatg aaaatttaaa gaatgtaaat aatagacata aagaattatc
     6721 aattaatttg caggattcaa ttagtgaaca caataaacta gaagatgaaa tacagaaaag
     6781 taaatataat ttaaatggat ataattcaaa gttaaatgtc tatatagata tggaaaatca
     6841 ttatgaagga tttaatagag gagtaaaaga agtactaaaa aataaaaatt taaaaggagt
     6901 tcatggagca ttaggacaaa ttataaatgt acctgaaaaa tatgagaaat ctatagaagc
     6961 agctcttggt gcttatatgc aaaatataat aactgataat gagtttagtg ctaagtctgc
     7021 aataaactat ctaaaacaga ataatttagg tagagtaaca tttcttccat taaatataat
     7081 taaatcaaat aaagtaagtc taggaaattt aaaagcaaat actaagttca taggaatagc
     7141 aagtgattta attacatttg atgaaaagta tagaaatata attgaaaata tacttggaag
     7201 aacaatactt ataaataata tagatgaggg cataaaattt gcaaaagaaa ctggtcatag
     7261 gtttaaaata gtaactttag atggtgaaat cttaaatcca ggaggttctt tgactggggg
     7321 aagtctaaaa actaatggga atatactatc gagaaaaaga tacattaatg aatatacaga
     7381 aaagataagt aatatcaaga atgaaatttc acatctagaa ttaaaaagag aatctttaga
     7441 caaagatgtg aaaaatatca aaaatgaaat tgattcacat gaaagtaaaa taaaagattt
     7501 agaaaaaagt ataataataa aaagtacaag tataaagaat gttgaatctg aaattgaatc
     7561 gttaaaaggt agtattacta aattagaaaa tgagaagaat gatttaaatt ctaatctaaa
     7621 ttatacactt gaaaaaagcg atgatgttag aaaagatatg gaagagttag atgatttata
     7681 caataaaaat aaagaaaaaa ttgatgcatt gaatgaggaa ataaaaagat acaatgattt
     7741 atatgataaa gaaaaatctg aatttgatga attgaactta agtttagtga agaaaacaga
     7801 agcttataat agtatagtta gagatataaa aagaatatct ggtgaaaatt gtgagctaga
     7861 agaaaagaat aaacaattag aagagtcatt aaattatgaa gaacatgaaa taattaaatt
     7921 acaagattct atagccactg aagaaaaaga aaaagaaaat ttaacaaaac aactagggga
     7981 tagtaataga aatcttgaaa ctagaaaaat agcaaaagat gatttaaaaa atagttttga
     8041 tgaaattaat aaagaattaa aaactataga tagacagcat atagagctta aagaaagttt
     8101 atttaaagtt ggtggaagat tagaaagatt aaaaactagt caagacacat atataaataa
     8161 gttgtttgag caatatgata tgactttagt acaagcattg gaaattaaag atgaagattt
     8221 ggatatagat agaaaattct tagaaagtct aaagagagaa ataagaagtc ttggaaatat
     8281 aaatataggc tctataaagg aatatgagga aataaaagaa agatacgatt tttatagcga
     8341 acaaaagcaa gatttagaag agtctatgga agagatagaa aaacttatac acactttaga
     8401 agaaaatatg aaatctgaat ttgaaattaa gtttgaagaa ataagtaaaa attttaaata
     8461 tgtatataag agattatttg gtggaggttg tggagaatta actattttag ataaagaaaa
     8521 tttattagaa agtgatatac taataacagc tcaacctcca gggaaaaaaa tgaaaaattt
     8581 aaatctatta tctggtggag agaaagcttt aacagcaata agtatattat ttgctatatt
     8641 gattacaaag ccaacaccat tttgtatatt agatgagatt gaagcgccac ttgatgatgc
     8701 aaatatattt agatttggag aatttttgaa ggatttatct aaagagactc aatttatatc
     8761 agtaactcat aggagaggaa ctatggaagc tgctgattat atctatggag ttacaatgca
     8821 agaaaaagcc atttcaaaag ttattagctt aaaattaaaa gaagctcagg aaataacaga
     8881 tattatataa cttagattga cataggagga ataacagtgt taaaaaaatt atttggtttt
     8941 ggaaaagaca aggaaaaaga aatagaaaaa aaagatgctg aagaagaaat agaagttgaa
     9001 gacagtgttg atagtttaga aaacttggaa gaaaccattt tttcaggact tgaagaagaa
     9061 gtgatagata aagtggaaga tattgaagaa aaaactgagg aaagtgtcaa taatgaaggc
     9121 actgaagaaa tcgaaaattt tgaaaaaata gaagttgaga tggatagtaa tgcaaaagaa
     9181 gtagaaaata tttcaaataa tgattctcaa gaagagataa tcgaagaatt agaaaattat
     9241 gatgaaattg agtctaaaga gatagaagat aaagaagata agaaagttaa cttgtttgaa
     9301 agactaaaac aaggattgac aaaagcaaag caaggaataa ctgatagaat agatgaagtt
     9361 ttaaaatcat atacaaaaat agatgaagaa ttacttgaag atttagagga aattttaata
     9421 acagcagatg ttggtgtaaa tacaactatg gatataattg aaagactaag agataaaata
     9481 aagcaaaaag gtattactga accaataaag gtaagagaag aacttaaatc tatagttgaa
     9541 gatattctta caaatgaaaa ctctacacta gatatagaac cagcaccatg tataatatta
     9601 atggtgggtg taaatggggt cggtaagact actacaatag gtaaactagc aaatagatac
     9661 aaaaaagatg gtaaaaaagt tttattagca gcagcagata catttagagc agcagcaaca
     9721 gagcaattag aaatttgggc aaatagaacc aatgtagaca taataaaaca tcaagaggga
     9781 gcagaccctg gagctgtagt atttgatgct ataaaagctg caaaagctag aaaaacagat
     9841 gtattaatat gtgatacagc aggaagatta cacaataagg ctaatcttat gaatgaactt
     9901 ggaaaagtat ttaaaatagt agatagagaa tttccagaag caaaacgtga agtacttctt
     9961 gtggtggatg ctacaacagg tcaaaatgca gttgttcaag caaagacatt taaagaagtt
    10021 gcagatataa caggtatagt acttacaaaa cttgatggaa ctgcaaaagg tggagtagtc
    10081 cttgcagtaa aatcagaagt agatgtacct gtaaaactta ttggagttgg agaaagtgta
    10141 gaagacctac aagatttcaa tgcaaaatca ttttcagatg cattatttgg taactaatgt
    10201 tcttttaaaa ataatggttg acaagagtgt tgttatgata taaaatacaa tagtgtaaag
    10261 taaataacct ttacagtata ctgctaggaa gtgtaagata tgaatataga aaaaatggtc
    10321 gaaattggat tgttatttga acagtataaa gaattattaa ctgacaagca gaaagaaata
    10381 gttgctctat actatgaaga agattattct ttaggtgaaa ttagtgaaaa tctgaatgta
    10441 tcaagacaag gtgtttatga taccttaaag cgttcagaaa aaatactaag agattatgaa
    10501 gaaaagttac acttagtatc aaagattcaa gagcaggaaa aaaatattaa gattataaaa
    10561 gataaaatta ttgacattaa agaagattta ttgcacaata gagattgtgc taatttaatc
    10621 cctaagctag aaaatataga agatgtatgt agggagatga taaaatgata tttgaaggat
    10681 tgtctgataa actacaaggg gcatttagta agttaaaatc taaaggaaaa cttacagaag
    10741 tagatgtaaa aaatgctatg agagaagtta aacttgctct tcttgaggca gatgttaact
    10801 ttaaagtagt taaagatttc ataaaaaaag ttcaagagag atgtgttgga caagaagtta
    10861 tgcaaagctt aactccagct cagcatgtaa tcaaaattgt aaatgaagag cttacaagtt
    10921 taatgggaga tgtacaaagt aaagtaatga tttcctcaaa gcctcctaca attttaatga
    10981 tggttggatt gcaaggtgca ggtaagacta ctacagcagg aaaacttgga ggatatttta
    11041 aaaagcaagg taaaaaacct ctattagtag cttgtgatat ttatagaccg gcagctataa
    11101 agcaacttca agttgttgga gaaaaattgg atatacctgt attcaatatg ggagataaag
    11161 aaaatccagt aaatattgcg aaagcaggtt taagccatgc gattaaaaat gctaatgatt
    11221 tagttattat agatacagct ggtagacttc atatagatga agtacttatg gctgagttaa
    11281 agtctattaa atcagaagtt aagccacatg agatactttt ggttgtagat tctatgacag
    11341 gtcaagatgc tgtaaatgtt gcagaaagct tcaatgaggc acttggtgtt gatggagtag
    11401 tattaactaa attggatgga gatactagag gtggtgctgc actttcaata agagcagtta
    11461 cacaaaagcc tataaaattt atggggatgg gagaaaagct agatgatata gaacctttcc
    11521 acccagatag aatggcatca agaatattag gtatgggaga tgtcttaagt ttaatagaaa
    11581 aagcacaaga aaatatagac ttagagaaag ctaaagaatt agagcaaaaa attaaaaaac
    11641 aagagcttga ttttgaagat ttcttacaac aaatggaaca aatacaaaat atgggacctc
    11701 ttgacaagat acttggtatg atacctggta tgggaaatgt aaaagaccaa cttggagatg
    11761 tagatttgaa tggcaaagaa atgaagagaa caaaagccat aattcagtca atgactacat
    11821 atgagagaag aaacccagga gtattaaatg cttcaagaaa gaaaagaata gctaagggca
    11881 gtggaactag tgtacaagat gtaaatagac ttataaaaca acttaatgaa atgaaaaaaa
    11941 tgatgaagat gtttgctggt tcacaaaaga gtatgaagaa aaggggcggc ttaggcggtt
    12001 tacctttttt taaataaaat ataaaaaagt aaaaattatt cggaggtgac tgtaatggca
    12061 gttaaaataa gattaaaaag aatgggagct aacaaaaaac ctttctacag aatagtagta
    12121 gctgattcaa gagctccaag agatggaaaa tttatagaag aaataggata ctataatcca
    12181 atttctgaac ctaaacaagt aagaataaat gatgaaaaag ctataaaatg gttagctact
    12241 ggtgctcagc caacagaagt agttaaaaaa ttacttgtaa aaaacggagt aatagaaaaa
    12301 ttcgaagctt ctaaacaagc aaaataattt tttcttggca attagtttaa gtaagggctc
    12361 attgctaagg ttactttagg agatgaaata tatgaaagag ctagttgtag atatagctaa
    12421 ggctctagtt gataatcctg actcagttgt tgttgaggaa tttgaagata atgatggtat
    12481 cgtcttaaag cttacagttg ctcaagagga tatgggtaag gttattggga agcagggaag
    12541 aatagctaaa gctataagaa ctgtcgtaag atctgttgca aatagagaaa atataaaagt
    12601 ttctcttgag atagtataaa ataggaggat taggtataat gcctagtcct ttttatataa
    12661 atgcgtagtt taagatttta tgatgatatg accacataag attaaatttt ttaagaaggt
    12721 gataacatga aagaaaaatt aactcatttt aaggtaggtc aaatagtaaa tacacaaggt
    12781 ttaaaaggtg aagtaagagt ttatccactt actgatgata tagagaggtt tgatgaatta
    12841 aaagatttct atctgggaaa agatttaaat aataaattgg ctgttgaaaa agtaagatac
    12901 aaaggttcta tggttataat gaagataaaa ggcatagaca gcatagagaa agcagaaaaa
    12961 ctaaaaaata aatttatgta tgtatctaga gaagatagca gagagcttga agaaggcgaa
    13021 ttttttatag cagatatgat tggaatggaa gttttaactg tagatggaaa atatgtagga
    13081 atactagaag atgttcttca atactcagca aatgatgttt atgtagtaaa aggtgaagaa
    13141 gataaagaat ttatgatacc agctataaaa aaatttgtac ctacaataga tattgatgaa
    13201 agaaaaatga ttatagaccc tattaaggga atgatagatt aggtggtaat atgagatttc
    13261 atataatgac actttttcca gaaatattta attcgtacat ggatgaaagt ataatgaaga
    13321 gagcagttga aaaaggtata attgaggttt atatatataa tataagggat ttttctaata
    13381 ataaacataa aaaagtagat gattatccat ttggaggagg agtggggatg gtaatgactc
    13441 ctcaacctat atatgacaca tataagcata taataacaac tcacaatata agtaatccaa
    13501 gtgtaattta tcttactcca aaaggtaagg tatacaatca atctatagca aagcagatgt
    13561 ctttaaaaga agatataata ctattatgtg gccattatga aggagtagat gagagaatca
    13621 ttgatttaat agttactgat gaaatttcaa ttggagatta tgtattaact gggggagagc
    13681 tcccagcact tataatgata gattctatat caagattaat accaggtgta ttaaaccaag
    13741 aagaatcatt tgaagaagaa tcatttaaag ataacttatt ggaatatcct cattacacta
    13801 gacctagaga ttttgaagga ttaaaagtac cagaagtgct tttatcaggg aatcataaaa
    13861 agatagatga gtggagaaga gaagaatcta taaggataac caaagaaaga cgatttgatt
    13921 tatataaaaa gtctaatgaa aaataaaaat ataatgaaaa attaatatat atgtatacaa
    13981 taaagtacct ataaatggaa ttaaaaacat aaaagtgctt gtaataacta ttgaagtata
    14041 gtataatata aaatgttgaa aatacgggcg ttcctctgtt tacactggtc gggtagttaa
    14101 gaacgtctat gatatctagg aggaagaaag atgaatgaaa tgctaagagc aattgagcaa
    14161 gaacaattaa aaaatgaagt tcctaatttt ggacctgggg acacagttaa agtacacgtt
    14221 agaattatag aaggaaaaag agaaagaata caagtattcg aaggtgtagt attaaagaga
    14281 caaggtggag gagcaagaga aactttcaca gttagaaaaa tgtctttcaa tgttggtgta
    14341 gaaagaactt tcccagttca ctctccaaaa atagaaaaaa tagaagttac tagaaaaggt
    14401 aaagtaagaa gagctaaact aaactactta agaggtagag taggtaaagc tgctaagata
    14461 aaagaagcta gaaataaata attaaaaact ttacttataa atggggggct gatttatcag
    14521 tcctctttct attttaatga aaattacagc aaataattat actagaaata aatagaaata
    14581 tttgttgaaa atatacatta tttatctatg aatttatagc ttttaaaatg aaaaataaaa
    14641 ttggtaaata ttggattata ttttgtaacg atattaaaaa taagagaaca gtattatgta
    14701 aagtatgacg ttaaaaagat gacaatatta tagtttgtga agatagattt tttaagtgga
    14761 attagaatat aataatataa tattaataag gagtgataca tgtgtcaaat gaatacgaag
    14821 agtatcttat ggatgacaat ctacatataa actggtatcc aggtcatatg aaaaaaacta
    14881 aggagttagt aaaaaataac ttaaaattga ttgatgtagt aattgaactt ttggatgcta
    14941 gaatcccgtt tagtagtaaa aatccagaca tagacaggct tgtaggagat aagccaagag
    15001 ttgtcgtact taataagagt gatatggcag atagggataa attgaatcaa tggattgaat
    15061 actataagaa aattaatatc aaagctatac cagtagatac aataaaaggt gttggtataa
    15121 ataatataat cgaagaatgt aaaaatgtaa ctagagaaaa aatgagttct ttaaaagaca
    15181 aagggagaaa agaaagagct atacgaataa tgatagttgg agttccaaac gttggtaaat
    15241 cgtctcttat aaacaaatta acaggtagaa aaagcactca aactggagat aagccaggag
    15301 taacgaaagg gaaacaatgg gttagattaa agggaaatct tgaactttta gatacacctg
    15361 gaatactttg gcctaagttt gaagaccaag aagttgcatt aaatttagca tttagtagag
    15421 ctataaagga tgagatttta gatactgaga ctttggcttt aagacttata gaaaaactta
    15481 tgaaaataga acctgagaag ttgaaagcca gatataagtt agattgtcta ggagaaactc
    15541 caattgaaac tatggatatg attggccata aaagagggtt tataacaggt aaaaaagaac
    15601 tagattatac acgcatagcc gcaactgtat taaatgaatt tagagatgga aaaataggaa
    15661 atataacatt ggaagttcca gaaaacgtta aaagatagat tttaaaacaa aatcaaagat
    15721 ttaatgttaa aaaaataaaa aagcctttta gcaaaggctt ttttattttt ttgaaatata
    15781 gacaaatatc tccatataaa agtataaatt aggttagaaa acctttaaaa atattaattt
    15841 aagacaaata cacctaatat tttgaaaatt aaaatgagtg tgattatttt aaattttagc
    15901 atattgactt gcttgtttcg tataatatat aataagcaca aataaataca atatattaaa
    15961 aatttaattt atttaatttt attaatttaa taataagttc ttattgttta atttaattta
    16021 tttatgcaag cgtaaacaat cgagacattt tattaattta ataaggttaa aaagggggat
    16081 tttataatgg gtaagaccaa ggatgttata gtatttggat ttgcgttatt tgcaatgttc
    16141 tttggagcag gtaacttaat atttccacct tacttgggaa taattacagg tccagaatgg
    16201 ttaattgcct tcttaggatt tacgtttgct gatgctggat tggcacttct agcagtaatg
    16261 gctacagcta aattcgatgg aaacgtagtt gaaatgttta aaagatgtgg tatgaagtta
    16321 gggattctta taggttgtgc agacatttta tgtataggtc cattcttagc tataccaaga
    16381 acaggtgcaa caacttatga aatgggtata atgcctttat ttggaacttc aattccagtg
    16441 ttattattct gtatactttt ttttgcgctt tcatatgtac taacaataag accttctaag
    16501 gtcgtagata tagttggaca gtttttaact ccagctcttt taatagctct agcatttata
    16561 attataaaag gtattatatc acctcttgga gatatagtag ataaacctat gatacctaat
    16621 gtatttgcag aaggtatagg tcaaggatat cagacaatgg atgcttttgc agcaatagca
    16681 cttgcatcag tgttaatagt atctttaaat gataaaggat attctactat tagtgacaag
    16741 ttaaaaatga ttggtaaggc gggggttctt gcttgtggag gattagctct agtttatgga
    16801 ggattatgtt tccttggagc aactgtatca acaatgtatg gaacagatgc agtacaatca
    16861 caagtaatag taaatataac tgaaggatta cttggaaatg ttgggaaagc gattttagca
    16921 gtagtagtat cattagcatg tttaactaca tctataggtc ttacatctgc cacaggtcaa
    16981 tatttctcta gacttacaaa aggtaaattg agttatgaaa aaatagtatt agctgtatct
    17041 gtattcagtg cagtagtagc atcttttggt gttggaacta taataaagat agcatctcct
    17101 atactaagta tagtttatcc accatcaatt gttttaataa ttttagcttt cttcaatgaa
    17161 aaaattaaga atgataatgt gtacaaagga gcagtatata tgtcattgct tgtaagtata
    17221 ctaacagtta tatctagtta tggagtagat gttccagtag taaaatcttt acctcttcat
    17281 tcattaggat ttaactgggt ggtaccagtt ataatagctg gaataattgg taattttata
    17341 ccatcaaaga gtcaatctaa tacacttgga acaaactaag ttttgtttaa ttaaaatata
    17401 atcaattgta aataaatgag ttaactaaaa gtaaaaggag ttatttgtac aaataactcc
    17461 ttttattttg ggattctata attaattatt tgtatttaat atggaaaaag tataagaaaa
    17521 tatcaaaaaa atatatagtt tattttttaa caataataga gattaaaaaa gaattttaga
    17581 gtaattttat aaatttaagt atacatatta aatgattttt acaaaaatat aatataaata
    17641 aaattgtgac aataataaaa taataaaaca aaatttaaga catatgatta tataaatgta
    17701 ttaaataaat gagtaaattg ttttcattga gagatatatt aagaattttt agttaattat
    17761 gacatataag tatgtaaacg tgaatactag caactgcaaa gtttaagtaa gttggaagtt
    17821 agaaagtaat tgctatgaag aaaaataagt tgataaatca taataaggaa ttttcagaaa
    17881 aaatggtttt aagactaaaa gacaagaggt aaatctacgt gagtagaata aaaattgaat
    17941 aaagttaact gtcaatttag caaaataaat acaaaatact acaagaatta acgttaatag
    18001 gtgttatgat aaaaggaaaa cgatttttta agaatcataa ttttgaaaat taaaaaaatt
    18061 aaaaatgcta ttgactgctt ttttttcaga ttatataata aacacaaaat atttaaaatt
    18121 taaattgaat taaaaagatt atatg
//
LOCUS       DAGQWR010000084        17894 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.084, whole genome shotgun sequence.
ACCESSION   DAGQWR010000084 DAGQWR010000000
VERSION     DAGQWR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.084"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            628..1137
                     /locus_tag="MIW95_002464"
     CDS             628..1137
                     /locus_tag="MIW95_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBY3151784.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            1165..1359
                     /gene="rpmI"
                     /locus_tag="MIW95_002465"
     CDS             1165..1359
                     /gene="rpmI"
                     /locus_tag="MIW95_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBY3151785.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1415..1771
                     /gene="rplT"
                     /locus_tag="MIW95_002466"
     CDS             1415..1771
                     /gene="rplT"
                     /locus_tag="MIW95_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBY3151786.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            2081..2626
                     /locus_tag="MIW95_002467"
     CDS             2081..2626
                     /locus_tag="MIW95_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151787.1"
                     /translation="MNEKLMDCFVNPIKCKLLLEVFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKEGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            2613..2786
                     /locus_tag="MIW95_002468"
     CDS             2613..2786
                     /locus_tag="MIW95_002468"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151788.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLHGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            3062..4987
                     /locus_tag="MIW95_002469"
     CDS             3062..4987
                     /locus_tag="MIW95_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY3151789.1"
                     /translation="MKIKFVIMPLVIFLIVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFISNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            5388..7292
                     /locus_tag="MIW95_002470"
     CDS             5388..7292
                     /locus_tag="MIW95_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY3151790.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFKQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEGKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
     gene            7680..8459
                     /locus_tag="MIW95_002471"
     CDS             7680..8459
                     /locus_tag="MIW95_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBY3151791.1"
                     /translation="MNYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKVFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVVLGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(8769..9590)
                     /locus_tag="MIW95_002472"
     CDS             complement(8769..9590)
                     /locus_tag="MIW95_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBY3151792.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDITVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLEDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(10315..11133)
                     /locus_tag="MIW95_002473"
     CDS             complement(10315..11133)
                     /locus_tag="MIW95_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY3151793.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNNTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDSVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            11301..11882
                     /locus_tag="MIW95_002474"
     CDS             11301..11882
                     /locus_tag="MIW95_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY3151794.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            11879..12073
                     /locus_tag="MIW95_002475"
     CDS             11879..12073
                     /locus_tag="MIW95_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151795.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(12237..13979)
                     /locus_tag="MIW95_002476"
     CDS             complement(12237..13979)
                     /locus_tag="MIW95_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBY3151796.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEDLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            14408..15784
                     /locus_tag="MIW95_002477"
     CDS             14408..15784
                     /locus_tag="MIW95_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBY3151797.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            16095..16766
                     /locus_tag="MIW95_002478"
     CDS             16095..16766
                     /locus_tag="MIW95_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBY3151798.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            16957..17754
                     /locus_tag="MIW95_002479"
     CDS             16957..17754
                     /locus_tag="MIW95_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBY3151799.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         7016 a         1997 c         2961 g         5920 t
ORIGIN      
        1 tatttattaa gtattgaata aaatattaca aatttagttg aaatgaaaat gataaaaaat
       61 gttgtgctat attttacaaa tcatataaat agagaaattg ttgtagcaca aagtaatagg
      121 tagtatatta gaatataata taggatttgt tgtgtagtat agtagcatat tagaatataa
      181 tataggattt gttatgtagt atagtagcat attagaatat aatataggat ttgttatgta
      241 tatagtagca tattagaata taatgtagga tttgttatgt agaatatata gagtcatgta
      301 gaatataaat atataaaagg attttgggta taaatttaaa aattaatagc aaaatgagta
      361 gtatagacat ataattagta gtaggaagta attaaatttt tcctaaacaa aaaaaatagt
      421 tgcaaataat ttgattaagt gatatagtaa ataaggtaag aataaaacaa agaagaagtc
      481 cgcttctcac cttacgccgg aggttagtaa ggtaaatata agttaaagct taattttaat
      541 ttaagtttgt ttaaatttgg taacttatat tgcggatgat tacgtcatcc gctttttatt
      601 ttataaaaat tttggaggtg tcctacaatt agcaaagaat tagcaatcaa tgaacaaata
      661 aaagataagg aaataagagt cctttcacca acaggtgaac aacttggagt aatgcctact
      721 aaagaagctc aagcaatggc taatagcaaa aacttagatt tggttcaaat ctcgccgaat
      781 gcgaatccac cagtatgtaa gataatggat tatggaaagt ttagatatga gcaagcgaga
      841 aaagagaaag aagctaagaa aaaacaaaaa actatagttg taaaagaagt aagacttaga
      901 ccaggtattg aacaaaatga tttaaatacg aaagctaata atgctataaa gttccttaaa
      961 aaaggggata aagtcaaagt tgaacttaga tttagaggaa gagaattagg acataaagac
     1021 ataggtaaag aagttatgct taagttttta gatataataa aagagtttgg agaaccaaca
     1081 aaagcccctg catttgaggg taataatatg gttgtaatta tagatccaaa aaaatagatg
     1141 attaactgag aggaggaatt tgtcatgcct aaaatgaaaa ctcatagagg tgcagcaaag
     1201 agattaaaga aaacaggaac tggaaagtta aagagagcta aagcttttaa aaaacatata
     1261 ttaactaaaa aatctgcaaa aactaaaatg aacttaagaa aatcaacttt agtaagtgat
     1321 ggagatgcca agagaatagc acaattatta ccatactaag atacagattg taaataataa
     1381 ctatagaaga gggatataaa aggaggtcat aacgatggca agagttaaaa aagctatgaa
     1441 cgctcgtaaa aagcataaga aaatattaaa acttgcaaaa ggattcagag gatctagaag
     1501 caaattatat agaccagcaa atacattcgt aatgaaagca ttaaaaaatg cttatatagg
     1561 aagaaagtta aagaagagag acttcagaaa actttggata caaagaataa atgcagctgc
     1621 tagaatgaat ggaatatctt attcaagatt aatgaatgga ttaaaattat ctggtattga
     1681 agttaacaga aaaatgttat ctgaaatggc tatacaagac ccagaaggat ttgcaaaatt
     1741 agcagaagtt gctaaagcaa aattagctta gtattgaatc atgagatgta aagtctttga
     1801 gttatataaa taattcaagg acttttttat tttttgaaaa atacttatta gtgtcatgta
     1861 caaccaattt tataatgata aataaaataa gcaatacaat agaattttat gagatttatt
     1921 ataagaattg ttacaattat ttatcatagc tataaaaagt catagtaaaa atgatattga
     1981 tgtatgttta tatatgtata aagctttcaa tcaatcttga attaatatta tcaacatgat
     2041 aatattatct tattaataat ataaataagg gggagataat atgaatgaga aactaatgga
     2101 ttgttttgta aacccaatta aatgcaagtt attgcttgaa gttttttcaa ataatcgaac
     2161 tacagctaaa caattaggag aaaaatttca agatatatca aaggcaacac tttatcgtta
     2221 ccttaataaa atgttgaaag atgatattat aaaagtggtt gaagaaaata aaataagggg
     2281 aactgttgaa aaagtatatg ctatgaagtt agatatagct gatgatgtga acaaaattat
     2341 agatgagaac tctggagaag aatatgctca actgtttact caatatatga tgggattttt
     2401 aagagaattt tatgaatacg ctgaaagaaa ggaaaaggat atcctaaaag agggttcagg
     2461 attttctgtc tgtccagtat atgctagtat tgatgaaata gttgaagtga gtaaaaaaat
     2521 tgctagtata ctagaacctc taagaaataa ctcgcacgaa gaaggcagaa gaatgcattc
     2581 tataggagtg ataatcactc cacctaaaga ggatgaaggg aaataaatct gatttacatg
     2641 aggtagagcc aatttacacg gaaaatcgtt ttatgattta catatgtatc cagagcaaag
     2701 tatctctgca tggattgtac ttcattgtac catttcatcc aatgacttgg agtggaacaa
     2761 tgaagttttt taatttttca tattaatgcc taaagaaatg aactttagac cacatatgca
     2821 attaggctac attatacaat tagaccagat atacagaata aaaattagta tttttatagc
     2881 agataaattg actgttattc ttcttaaaaa tcatctatta gttattgtta gcatattgaa
     2941 agaaattata gtaatccatt gaataaatga atattgccca taatcatata aactgctata
     3001 gtttaaaata aagcaggacg ttaaatttta tagtaatctt aaaatttaag agggggattt
     3061 tatgaaaata aaatttgtta ttatgccatt ggtgatattt ttaatagtat tatttgtggg
     3121 gtgtcaaagt acaaaagata cagaagttaa agcaaaagat acagaaatta aaatattagg
     3181 tacaggtgat ttgcattcaa ttttgacaga ctctatggtt tcatatgtaa atgaggaaag
     3241 ggaaaagaat aaaaatctgc ttatggtaga tgcgggagat ttctatggta cacaaagtag
     3301 agaaatgtgg gaatggtctt ctggaaaaaa gttaattaat attaaaagaa atggtagagc
     3361 agagtatgat gacataatta aaagtagtaa agatgaagta cctatagtta aagatatggc
     3421 aaagttgaaa tatgatgctg tagttttggg agataatgaa tttatttcaa atgataagca
     3481 aagtcttgat aaactagttt ctgattttaa aaataataat atgccattgg tatcagcaaa
     3541 tatatatgaa caatctggag aaaattatgt acaaccatat gtaatgaaaa atattaagac
     3601 agatgagggc aatgtaaaag ttggaatact aggtcttact ataaaagaag taggggaaac
     3661 ccttggtctt gaggattttg aaaaggataa aaaagctaga gagcttggag aacaagctga
     3721 ttacaaagga aaattatatg caaatgattt agtagaagat gcaaagaaat ggataaaagt
     3781 tatggaaaaa gaaagtccag acataatagt tgcagttgta cactcagggg aagaacctaa
     3841 aacagataga aatccaggaa atagaataaa agagttagca actacagtgg atggaataga
     3901 tgcaattgta gctggtcata ctcatgaaaa aatagatgaa cacaaatata aaaataaatc
     3961 tggagatgaa gtaattgtta cacaaccagg ttggcatgga gatagagtat cagagattaa
     4021 tttcaaatta aataaagaga atggaaagtg ggacataaag gaaaagtcta gtaaattaaa
     4081 aataattgat aaagaagtaa gtatagttgc tactgcagat ttacattcac atttctctga
     4141 taaattaaca gttgaccttg ttaatgaacg tagtaaccca attgaacctg ttgtagtaga
     4201 tgcaggcgac ttcttagacc ctcaaacaga tgaaatgact caatggtata aagagtggaa
     4261 aagtataaga gataataata gtgataaaac gattagtaga tgcccaatgg tcatggatat
     4321 gaatcaggga atgtatgatg cagtagtact tggaaatcat gaatttgtat ctgaaaaaga
     4381 tgaattttgg tcagatgaac gccttttaga tgctgttata gaggattttg aacaggtggc
     4441 aatttctgtg ttatcagcaa atatatatag gcaatctggt aaaaattatg ttaaaccata
     4501 tattataaaa gatgtggaga cgagtgaagg taatgtaaaa gtaggaatat tgggacttac
     4561 tataaaagaa gtaggtaaag gtcttaaaaa tgtaaactta caagagcaat ttcaatataa
     4621 ggataaattg tatgcaaatg atttagtgga agatgcaaag aaatgggtaa aagttatgaa
     4681 aaagaaaaaa ccagatataa tagttgcagt tgtacattca ggagaagaac caaaaaatcc
     4741 aaaacaccca ggaaatagaa taaaagagct agcaactaca gtagatggta ttgatgcaat
     4801 tgttgctagt catactcatg aaaaaataga tgagcatgaa tataaaaata agtctggaga
     4861 aaaagttata gtgacacaac caggtgagca tggtaaatat tattctaaaa ttacctttaa
     4921 agtaaataaa gaaaagggtt cgtggattat taatgataaa ttcagtaaaa caattgaggt
     4981 tgaataactt tataaaattt atagataaag taaattatct gatattgaca tgatatataa
     5041 gataaagttt ctaatattag tataatgcat aatgtgaaat attttaataa taagagaata
     5101 aacaagacaa ataaaaatat tttaacatgt aagatatagt atgaactatt atactaatgc
     5161 ttactttata atttgaacat gttagaataa gtcttatttg aaaattttag tgtattagag
     5221 taagtattat tttaccagta aaaaatattg agaactatat gtagatttgc ttaaaaagga
     5281 atttacatat agttcttttc gtataataaa tagtacaatc ttatatggta aagataaagt
     5341 gtgatattaa attttattat catccaaaat ctaaggagga gtctcttatg aaaataaaat
     5401 ttattatagt agcatcaata atatttttaa tagtattact tgtggggtgt caaagtacta
     5461 aagacacaga aatcaatgta ctcgccacaa gtgatttaca ttcaattatt ccagataatt
     5521 taatttccta tgtagaagag gagagaaaat atgatgagaa tctacttatg gtagatgcag
     5581 gagatttttt tgacatgcaa agtggtgaga tgaataagtg gtttactggt caaaaattag
     5641 taagatttaa agatgggata ccagagtaca aaaaaatagc tgaaccacgt gaaggagaag
     5701 ttccattggc taaaaaaatg gcaaagctga agtatgatgc agttacattt gggaatcatg
     5761 aatttgtatc taatgacaag cagagccttg ataaattggt ttctgatttt aaaaataaca
     5821 atataccatt agtatcagca aacgtatatg aacaatctgg agaaaactac gtaaagccat
     5881 atgtaatgaa aaatataaat actgacaagg gaactgtaaa ggttggtata cttggtctga
     5941 ctataaaaga agtaggggaa cgttcaaggt ttaaaagttt taaacaagat aaaaaggcta
     6001 gagaacttgg ggaacaacct caatacaaag ggaaattata tgcaaatgat ttagtagaag
     6061 atgcaaataa gtgggtaaag gttatggaaa aaaaagaaaa gccagatata atagttgcca
     6121 ttgtacattc aggtgaagag ccaaaaaatc ctaaaaatcc aggaaataga ataaaagaac
     6181 tagcaacaac tgtagaggga atagatgcta ttgtagcagg tcatacacat gaaaaaatag
     6241 aacagcatac atataaaaat aattctggtg aagaagtaat agtaacacag cctggagctc
     6301 atggtaatag tatatcaaag attaatttta agttggataa gaagaatgat aagtggttta
     6361 taaaagacaa atatagtgaa ttaaaggttt ttggaaagga agtaaatata attacaactt
     6421 ctggtctgaa tccaaaattt tcagatgaaa tggcagttaa tttgtttaat gaaagagata
     6481 gagatgttca acctatcttt atagatactg gagatttttt aaatactaat acaaaagaaa
     6541 tgactaagtg ggctaaagag tggcaataca atgaaaataa aggcatctat aaaaaaataa
     6601 atgaatggcc aattgtatat cttggttatg atgctgtagt acttggtagc catgaattta
     6661 cagaggataa agagactttt gagggaaaaa aatctacatt ggattatatt atagaaaact
     6721 ttgaagaaat gaagatacca gtattatcag ctaatatata tgatgaatct ggagaaaatt
     6781 atgtaaaacc ttatataatt cataatgtag aaactaggga aggaaatata aaggttggag
     6841 tattaggtct tactactact gataaggatg aagaagactt aagtagctca agcttaagca
     6901 aagactcaaa gacttcgaaa attcctgaag aaacaggaaa gttatatact aataatctag
     6961 taaaagatgc aagcgaatgg gtaaaagtta tgcaaaagga aagtcctgat gtgattgttg
     7021 cagttgtaca ttcagataat aatagtactc taaaaaatcc aagtagtgaa gtcaaaaaac
     7081 ttgcaatgtc agtagatgga atagatgcta ttgttgtagg tcatacgaaa aataaggtag
     7141 aagaacatat ttacaaaaat aagtctggag atgaggttat agtaacacaa tctggagaag
     7201 attgttattc aaaaataagt cttgaactaa gaaaagaaaa tggcaagtgg aatatcataa
     7261 acaaatatag taaagcaata aaaatgaagt gaatttattg atatataaac cattattaac
     7321 actgattttt ataatataat aaatactgat tctacaatat aattaacact acttttttaa
     7381 atataatcaa tactgattct acaatattat taatactact ttttataata taatcaatac
     7441 tactttttac aatataatca atactgattc tataatataa ttaacactac tttttaaata
     7501 taatcaatac tgattctata atattaacac tattttttat gctatataac aattattgaa
     7561 aatgatataa atataatatc tcaaatgata tgaatataac atctgaatag agggtgtttt
     7621 atttgtaaaa aataagttta cagtttgagc gtataaactt atggtaaagg agaataatta
     7681 tgaattataa gaagattgca attataacgg gtgctaatag tggttttggt aaagagtttc
     7741 taaagttatt aataaatgaa gaagaaataa cagaaatatg ggcagttgca agaaacaagg
     7801 cgagattaaa tcaattagta gatgaatttg gaagtaaggt aaaagttttt tcaaaagatt
     7861 tatcaaaaat agaagaagta aaagaaattg gtacattttt aagtaaagaa aatgtatgta
     7921 taaaatacct tataaacaat gctggttttg caaaattctg ttcatacaat gatttaagta
     7981 tagatgaatc tattaatatg attgatttaa atattagtgc tgttgtagta ttgggactta
     8041 tatgtattcc atatatgcaa aagggaagcc atattatgaa tatttcttca caagcatcat
     8101 tccagccatt gccatatcaa aatatttata gttcaactaa agcgtttata agaaattata
     8161 caagagcatt aaatgttgaa cttaagagta aagggataaa tgcaattgct gtatgtccag
     8221 gttggatgag tacaaatcta tttaagagag gtatagttgt agctgaaaaa gggacaaaaa
     8281 atttcccagg catagtgtct ccagacgttg ttgccaaaaa agcattgaag gatgcaaaga
     8341 aaaataagga tatttctgtg tatggtataa atacaaaatc cagtcattta ttagcaaaac
     8401 tattgccaca aaaaatgatg atgaaggttt ggttaatgca acaacatatt aaggaataag
     8461 agtttcggta attaagtatc agttggagat tcaatctatt atatattata agaagttttg
     8521 ttgagcaaat aataattgtg acaaaacggc ttatctagtg ccgataagcc gtctataatg
     8581 tataaaaaat atatttttga ttttacttta tttagatatt gaagtattaa aatatgtagg
     8641 acttgaaatt ttataaagat aaatactatg atattttgta aaaactgaga tatgttgata
     8701 ttacatagtt taactatact gttaaattga agttttctta ttttgcatta tgcttattac
     8761 ttaatgattt agactataat ttccatacca tcatatgcaa aataaatgtt atcaagtttc
     8821 ttttctaatt cactgtaata accatatgat tttccccaca tttcgtcaat atgtgtaatg
     8881 ataatctttt taattctata atagttttta atttcaacaa tctcatctaa agtaaatatt
     8941 tcatctttaa atggtgtgtc ttcaagtttt gtaccatcat ttaatatacc atcatcagaa
     9001 actagactta ttattaaaac atcagcatca aagtacatat cactatgagt aaatggcttg
     9061 ggattacagc aggcatatat gacttttttt tcattctctt tgataacata gaaggttata
     9121 ttcctgtctg ggttattgtt aatcaaatca atatcaaaat tatttatctt tacatgcgaa
     9181 gtttcattaa tagaaatgca atttataaca tcaccataat atttaagaca atcgagattc
     9241 gttcggttta tatcttcaat tacttcagga agagcataaa agccaattgg attattttct
     9301 cctgtgataa agtcacatcc tattttttcg acaattctaa tacccttaac atggtctggg
     9361 tcatggtgag aaattgaaat atgttttact tccgatattt tttgtctgtt acaagctact
     9421 gtaatatctt caggtgtgtc tatcagcatt tttatatcat caatatataa acttggtcct
     9481 aatcgttcat atcttccgcc tttttgcctg gcttcttcac atattttgca tttacaaaat
     9541 gaacttggaa taattgacat accgccacta ccaacaattt ttaatttcaa agttattaca
     9601 ccaccttcta aaaatattag attatataaa taatctacaa tcacaactga ttaacaaaat
     9661 tatgagttaa taaattatat cacatgtaaa tatgaaagaa tagtatgaaa tttttataag
     9721 attatattaa atatttctca atgaaattga ctttgtatat gagccgaatg actttattca
     9781 atatagataa gacatttttc ttgtaataag atatgattat tctataaggt tcatgtaatt
     9841 ttgatataaa atattgatta ataacatata tgttattggt gaacatattt ctatagaatg
     9901 gaatatataa ttttttatat ttatcattgt attattatac aaagaaagtt aaaaagagta
     9961 gagtacaaag gaattatttg tactttactc tttgttttgt taaaaactaa tttatattag
    10021 tgattcaaat atttaactag agtataaggg ttgtatataa tcttataagt aatttatctg
    10081 acaaattaag ataatatcct aatcaaattc tagtcattcg aacataacat cacaagtgat
    10141 ttatctgaca aattaagata atatcctaat taaattgtag atatttatac ataacactaa
    10201 aagtaatttg tttaacaaat taagataata tcctaattaa attgtaggta tttatacata
    10261 atactaaaag taatttgttt aacaaattaa aatattatcc aattaatata aaaattactt
    10321 ctttttaact gggaagcata cctctgttac ccaattatca ggattactat cgttgccagg
    10381 acttacatga taaatattaa acataggacc atccacatca tagttattat ctgatatcca
    10441 attaccaata gcctcacaag cgtctgcaac ttgattgaaa tttccattaa caattgcagt
    10501 tgcagcagtc acacttggaa cagtcttaaa tttaacatgt tcagtatctt tatactttcc
    10561 agatacagca acttgtactt caacatctac agaatcttct ttatatccag tatcataaaa
    10621 gactgcttta gaatactttg ggggttcaat ttgaacattc tggtcttttg tctctaaaaa
    10681 tgcttgatgc cataacattc cttcgtcttg ataagttggt ataacccttc taagtgtcat
    10741 catatatttt ggtgcaaaat tctttatagt tacatcataa tttttcataa tatcatttcc
    10801 tccaattctt ttaatagtgg tttctatttt taatagtttt ttctgtgtat cttctaattg
    10861 ttcttttact tgagaatggt ggatatttag atattttata agactttctt tatcgttata
    10921 ttctgtcaaa atttctttta tagaatacaa gccaaagccc atatctttta gtgcatggat
    10981 tctatttgta gtagaaagtt ggtctgcact atagtatcta tatccattag tgttattcgt
    11041 atgagaagga gttaaaagtc ctatttcatc ataatgacgt aacatacgaa tactaatttt
    11101 acttagcttt gaaaaatcgc ctatcttaaa catgtttttg tctcctttat aaaggtactt
    11161 ttagcttaat attaatataa accatatcat aatgttagag tcaactaaaa ttataattaa
    11221 attatattga catatgatga aaaaaatatt atactgaaca tgttcagaac ctaaaatgtt
    11281 aattattttg gaggtctatt atgaaaagta atgataaaga tgctagagaa cgtataattg
    11341 aagttacatt gaatctttta aatgaagtag atgatattga ggagattaca gttagaaaaa
    11401 ttgctgagcg tgcaaatgtt ggagttggtt taataaatta tcattttaaa acaaaggata
    11461 atttgttgag tactgccata ggagatgtga tgtcaaatat aatagcagag ctatatgacg
    11521 atagtgtata tactctaaga cctattgaag atttgaaaaa tttattaaaa aaattgtgtg
    11581 atactggatt acattatgaa aaagtgcttc cttttgtgtt gaatcaatgt ataaccaatg
    11641 gagatatgca agcagaactt gatattgtcc ctatgcttcg taaaattttt ggaaataaaa
    11701 aagatgaaat gagtctaaga atcattgccc tgcaaattat tttaccaata cagatttctg
    11761 ctttatctac agaatcattt cagttataca gtggtataaa tataaagaat aaatatgagc
    11821 gagataaatt cattgatatt ttaatagaaa atattattgg agaggatgtt gatgtaagat
    11881 gaaaattata actattggtt caagtcttat cactgtttta ctttttcttt caactatggt
    11941 ttgtgggttc tggattaaga ataataaggt aacagatgca agttctatta agttccatat
    12001 gaatagtgct atttttacag ggatattttt attgatttcc acaatccttt taattatata
    12061 tataaaaaaa taataatatt attaataaat aaagtatcag aaattataaa gacaagagtc
    12121 ttaaaaattt ctgatacttt accacataat tttattaaac attaattgtg tgtgaaaaaa
    12181 tatcacttat ttcaatctaa tactaaatct taagagtgag tataaagtat tagaaattaa
    12241 gaagcatttt ttagttcaag tggattatct ataccaaaat aatcatataa agtagagctt
    12301 atttcctcta aatttccatc ttggaaaggt aattgtaaat ttaccttttc taccacatct
    12361 ttaaatccag catcagtatt ttgatttaaa aaatccatat atacatcaac agcttttctg
    12421 taatcttctt gtgataattg ccatatctga agtgatacat cagatgcaag accataactc
    12481 aagtaataaa aaggaacttg gaagttatga gatacaagta tccaaaaagg agcatttttt
    12541 tctctaagta tattattgga aactccatat tcttcctcta aatcaaaaaa caatttattg
    12601 atatcattta gactcatata tggattcttg tatataattt cttgaaattc atcataaaga
    12661 caagcatcaa taatagtatt taaaacaatc gataagtgtt ctttttttat agcttcagat
    12721 tgtttaccaa aaaaatcatc aaaatattta taaaatagaa tttctaaaga tgttgaatga
    12781 acttcacata catctatact tggttgcatc ttattattta agttaatact gttgtagtaa
    12841 ttataaaaat gaccaaactc atgagcaaaa ctagtaacac ctaaaaaaga attgtcccat
    12901 gtattaaaca agaaaggtgc tttatattta tttatataag tagtatatcc tccaggcgat
    12961 ttgttttttc cagaagaata atcgtacaag tcatattttt tcatatagtc gaagctctct
    13021 tttagttttg gagatatatc ttcaagtact ttatcaaact tcctaaatga gcgattttta
    13081 tatactttaa tataaataga agaatcagat ggtttagatg aaatctctct atataatgga
    13141 actatgtaat cttttacatc ttgtctaaat tttttagctt cttcttgaga atagtctttg
    13201 tttaggttca tataggcata atctatataa ttcttaaatc cttgcttact tgctatttca
    13261 gttcttgtct gtataagttc taagaatatg ttaccaaata taggattgta tttttttaag
    13321 taatctgaat aaattttcac atattcttca gggctaagat ttggtcttga caaggcttct
    13381 tcaaagtcta cctctatacc atctattgat actgtagatt tagctaataa gtcttcatat
    13441 tttacaacta ggtcttgctc ttttttaagt aaatcttcaa cttctttaga attaagtttt
    13501 ccaatgttct ttagataatt aaatgtatcc tttccaagtt ctttttctaa ttctttagag
    13561 aatttagaag ttacaaatct atcttgaaag tctttgtatg acatgtcaac atctactgat
    13621 ttactcataa gatacttata ttccttagag taaaattcgt ctgatgaatc tatgttattt
    13681 ctaatttttg ctatagtaag tgtactgtaa aaatcctgat aaattttatc aacttcatta
    13741 aataaattta attgttcctt agctgtttta gctgttaaaa gtttgtcgtt acacttgtta
    13801 atgttttcgc aaattgactg tatattagga cgcttatatt ccattttttc aaaaggaata
    13861 gttcctctaa cagaatcttt ttttgtatca ttagtataat ttcttatgta tgatgcaaat
    13921 ataataaaaa ctgcaattaa aattagaaaa acaaatattt tcttcttgga tttaaccata
    13981 aaaatcctcc aatcatggat atagttatta attattttac tatattaaat ataaaaaatg
    14041 catgttaaga aaatttaaca taaagatata gataagatta taaataatat aaaaaattta
    14101 acttcaaaaa agtatatgaa atttatactg gtaaaatgat aaataaattt taagtacaaa
    14161 atagtaaaag ctatgatagt tttagaccca ttatatgtaa aaaatataga tgtttttggt
    14221 gcaatttaaa ttaaaagata tatataaaaa tttaaaatat tccaaacaga aattataaaa
    14281 attactatca ttgtctttat gcaatattta tactataata gaataataat ttagaggaaa
    14341 gctctaaaaa taaacaaatt aagggtctta acagtagtga ttttatgtta aggaggtata
    14401 aagaaagttg aaaacaatag ttagaagact tattctctac gcagatggaa tgcgtcctac
    14461 tcaaataatg gtatctgggt ttgctgcgat tattgtaatc ggagcattgt tgttaacact
    14521 cccgatagcg tcgcagagtg gagagagtat aggcttatta aatgctctat ttacggctac
    14581 ttcagcggtt tgtgttactg gtctagttat ggtagataca gcaacttatt ggagtttatt
    14641 tggacagata gttataataa cattgataca aattggtggt cttggattta tgaccgtagc
    14701 cacaatgttt tcacttatgg cacgaaaaaa aatacaacta agagaaagat tattaataca
    14761 agagtcatta aatcaagctg atttatcggg acttgtaaga cttacaagat ttgtattaat
    14821 aataaccata acaatagagg gaataggtgc actggtttta tcaactgtct ttattccaca
    14881 atttggtttg tctaaaggga tatggtatag tgtatttcac gcaatatcag cgttttgtaa
    14941 tgccggattt gacttgatgg gcagtgtaag tggcccattt acatcgttaa attcttatgt
    15001 aaacaacttt acagtatcaa tgactgtttg cgcacttata gtgcttggag ggctaggatt
    15061 ccctgttgta cttgatattg taagaaaaag aagattttca aaattaaatg tgcattctaa
    15121 ggttgtattg ttttcaactg ctacacttat ttttgtgggt gcattattta tttttcttat
    15181 agaatttaat caaaaagcta ctatggcgga cttgccttta aagggaaaag tactatcagc
    15241 aatatttcaa tcagtaacag caagaactgc tggttttaat acacttgatt tagctacttt
    15301 acgagaaagt agtgtatttg taatgataat tttgatgttt attggagcat cacctgcatc
    15361 aactggtggg ggtataaaaa caacaacact agctgtttta atcattacag ttagaagttt
    15421 tctatcagga aaatcagata tagaagcttt tgaaagaaga ttagctccat caactataaa
    15481 aaaatctctt ggtatatttg taatcagtat atcagcagtt atttttggaa cattgattat
    15541 ttcaataact cagcctaatt ttacattagt tcaatcagct tttgaggtta catcagcact
    15601 tgctacagtt ggttcaagtt tagctggaac tccaaattta aatgctttag gtaagattat
    15661 aataataatc tttatgttta tgggtagagt tggttctctt actttattta tggctatact
    15721 ttctggtggt agaagaaaga gtcaaccaat cagatatgca gaaggtaaaa ttatggttgg
    15781 ttaagttacc aaggagtaat ataaatttat aatggattta gagtagcact gtaaatctgt
    15841 tgataaaata aatgagccta gatgtaatta tatgaagaaa taaaatcaac taataaacaa
    15901 aattaattaa tgaaataaaa aaacagggaa ataaaataca aataaaaagg taaatcaaaa
    15961 ttaaattaat ctgtgtatat aaaattatat attgtgtata agttttgtat gtgtaaagta
    16021 tataagtttt gtatgtgtaa acaattaatt taagagatat tatataaatt gtgtattagg
    16081 gaggaagttt cgagatgaag caatatatag tcattgggtg tgggagattt ggaagttcag
    16141 ttgcgtctac tatgcatctt ttaggacatc aagtaatggc aatagacaaa aatgaagatt
    16201 cagttcaaag tatatctgac aaggtaaccc attcacttat agtggatgtt actgatgagc
    16261 aagcgttaag gtcattaggt ttaggtaact ttgatgtagc agtagttgca ataggttctg
    16321 atataagggc atctataatg gcgactctta tagccaaaga aatgggtgta gagttgataa
    16381 tatgtaaggc aaaggatgaa ttacaagcta aagtgcttta taaaattggc gcagatagag
    16441 ttgtatttcc agaaagagat atgggagtaa gagttgcaca caatttagtt tcggataata
    16501 tattagacca tattgaactt gacccagagt attcaattgt tgaaatcgta actccaaata
    16561 gttgggttgg caagacactt atagagcttg aattaagagc tagatatgag ataactgtac
    16621 ttgctataaa aacaggtaaa aatataaatg ttacaccttc tccagatgag gaacttacag
    16681 ccggaagtat cctagttata atcggtcaaa atactagtat aacagcgata acatctggaa
    16741 ataaggggat aattagaaga agataattta ctatttaata tatatttaat tgcaatgaaa
    16801 gtaaagagta tcatataatt atgaatagtt atatgatact atttttattt aatcgaaggt
    16861 agtatttatt tgtaagatta gataaagaga agttaaatta aaagtaagga ggctgtgcta
    16921 atataaaaat ttataagtta ttagcattta gataaaatga ttacaaatat aaacagtaag
    16981 gataatgaaa agttaaagta tacaagagca ctattaaaat caaagaatag gaataaagag
    17041 tcaaagttca taatagaagg atacagaata gtaatgcttg cacttgaatg tatggcaaac
    17101 cttgattatg tatttatcaa tgaagaattt gaaaataaga aagaacatgt aaaactatta
    17161 gaagatttgg ataaaaaaaa cacaaagata tacaagacta ctaataaaaa ctttaaagaa
    17221 ttagtggata cagaaaatac tcaaggaata ataggtgtag tttcatttaa gaaaaaaaaa
    17281 ttaagtgaaa gtataaataa aaaagataaa tttgtattga ttttagatag aatacaagac
    17341 ccaggaaata tggggactat aataaggact gctgattctg ctggagtaga tgccataata
    17401 gcactaaagg gatgtgtcga tatatacaac ccaaaagtaa ttaggtctac tatgggttct
    17461 atttttgata tgaatataat tgatgcttca caagatgaaa ctgtggacat gcttaaatca
    17521 ttggatttta atatagtttc aagttactta aatacagaaa atttttatga caaaatagat
    17581 tatggttcaa aagtagcatt ggtgatagga aacgaagcaa atggaataaa tgaagaactt
    17641 gtatcaaagt ctgatatttt ggttaagata cctatatatg gtaaagccga gtcgttaaat
    17701 gctgcgataa gctctgctat actgatgtat gaaataaaaa aatacttaat ttaatgtatt
    17761 gtaataaata taatgtcatg ttataatctt gaattaaata gtagagtatc aaaaatagtc
    17821 aatatatatt aaaaaaataa ttaaattaat tatatattag atgtatatca tataaaaaat
    17881 gattgagttg atta
//
LOCUS       DAGQWR010000085        17730 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.085, whole genome shotgun sequence.
ACCESSION   DAGQWR010000085 DAGQWR010000000
VERSION     DAGQWR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.085"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            398..1732
                     /locus_tag="MIW95_002480"
     CDS             398..1732
                     /locus_tag="MIW95_002480"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBY3151800.1"
                     /translation="MRVVIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFDDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(1861..2778)
                     /locus_tag="MIW95_002481"
     CDS             complement(1861..2778)
                     /locus_tag="MIW95_002481"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBY3151801.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLNSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            2986..3870
                     /locus_tag="MIW95_002482"
     CDS             2986..3870
                     /locus_tag="MIW95_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBY3151802.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRIKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGKKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            4083..5924
                     /locus_tag="MIW95_002483"
     CDS             4083..5924
                     /locus_tag="MIW95_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBY3151803.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKNKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDYVVSKKVDEIKEQQEKILDKVIGATTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGERITSIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPILKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNQGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            6228..6935
                     /locus_tag="MIW95_002484"
     CDS             6228..6935
                     /locus_tag="MIW95_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3151804.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            6929..8239
                     /locus_tag="MIW95_002485"
     CDS             6929..8239
                     /locus_tag="MIW95_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3151805.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMAQE
                     NYLKDTTESEETGYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEKLEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            8356..9039
                     /locus_tag="MIW95_002486"
     CDS             8356..9039
                     /locus_tag="MIW95_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151806.1"
                     /translation="MKILYTENLSKHYGKGENLVRALDNVNLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            9032..11419
                     /locus_tag="MIW95_002487"
     CDS             9032..11419
                     /locus_tag="MIW95_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY3151807.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFELIKGKLPTKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKNDK
                     KIEKEFVISGILKTSEISEAGKYYYAIISELYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGGFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNLIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKAVISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            11698..12840
                     /locus_tag="MIW95_002488"
     CDS             11698..12840
                     /locus_tag="MIW95_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBY3151808.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIVGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLDRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALKLTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNENLLNLFNKYYF"
     gene            12859..13383
                     /locus_tag="MIW95_002489"
     CDS             12859..13383
                     /locus_tag="MIW95_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBY3151809.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYITEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            13483..14301
                     /locus_tag="MIW95_002490"
     CDS             13483..14301
                     /locus_tag="MIW95_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY3151810.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSELFAKKLEESLNPTQSVEKIVLYLEEMLLSILEEDLISNKSV
                     KLLNSFIYDNRYSNIKAFCDNTNINIKTLERICLKYTGYTPKILKRIYRFKMASSQLI
                     YNYKDNELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYNYL"
     gene            14379..14690
                     /locus_tag="MIW95_002491"
     CDS             14379..14690
                     /locus_tag="MIW95_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBY3151811.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(14993..16996)
                     /locus_tag="MIW95_002492"
     CDS             complement(14993..16996)
                     /locus_tag="MIW95_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBY3151812.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(17149..>17730)
                     /gene="recX"
                     /locus_tag="MIW95_002493"
     CDS             complement(17149..>17730)
                     /gene="recX"
                     /locus_tag="MIW95_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBY3151813.1"
                     /translation="YVDDKFFIAIFKELVYTFNLKKGNEINEEELKPILDDEMYIKAK
                     NKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKSYNLVNDSVLAQKIVNTNVN
                     LNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENAMYLAKKRYERVKKEDKKKI
                     YQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT         6973 a         1893 c         2933 g         5931 t
ORIGIN      
        1 aaaatcgttt taaaatctgt aatcataaga aattgtgata aaaatgaact ttccaatatt
       61 ttttctcctt ttatttaaaa ataaattttt ctaagtttaa atgataccaa tatattaaaa
      121 aaatgtcaat attatatcac aatttactta aatagagtaa aataaattga ttttgaaaaa
      181 tgtgattaag tatagaaaaa tagggtataa taaatgttta aagaatttga attgtggtaa
      241 gaaaataaaa aatagctttt ataatatttg atttcaaatt aaaatatgtt aaataaataa
      301 ctaaaaattg ttatgaaaaa tgtttacata gaatatatat caaatgaaaa gcttaaaata
      361 agtgaagaaa ttaactagac atcaaaggag agataaaatg agagtagtaa taattggtgg
      421 tgttgcagct ggtatgagtg ctgcagcaaa attaaaaaga ataaaacctg agtatgaagt
      481 tgttgtttat gaaaaaactg aaatagtatc ttttggtgct tgtggattac catactttgt
      541 tggtggtttt tttgatgatg ctgatgaatt attagcaaga acaccagaga aacttagaga
      601 agctggaatt gatttaaata tatttagaga agtcgttgag gttgatagtg aatctaagaa
      661 aataaaagta aaaaatataa aaacagatga aatatatgaa gattactatg ataaattgat
      721 gatagcaact ggagcaagaa gtattatgcc tcctataaaa aacataaaat tgaagaatgt
      781 ttctacatta aaaagtttat atgatggaga atatctaaaa aaacttttaa gtaatgaaga
      841 taataaaaga gtgacaataa taggagctgg ttttataggg ttagaagcag ttgaggcatg
      901 taagaaatta ggtaaagatg tagatgtaat tcagttagaa gatagaatat tgccacaagt
      961 atttgataag gagatgacgg atgtattaga agaagaaata aatagacata atgttaattt
     1021 gcacttggat gaaatggttg tagaactttg tggagaagat aaagtagaaa aagttataac
     1081 aaacaaaggg gaaatagata cagatgtagt aataattgct actggtgtga gacctaatac
     1141 cgagtttttg tcaaatacta atataaaaat gctaaaaaat ggagctatag tagttgatga
     1201 atatggaaga acttctgtag aagatatata ctctgctgga gattgtgcaa ctataaagaa
     1261 tatagtaagt aatgaaaatg tatatgttcc tttagctact ggagctaata aattaggtag
     1321 gatagtagga gaaaacctag caggaagaga agtatcttat caaggttcac tatcttcaag
     1381 ttgtataaaa attatggata tggaggcagc atcaactgga attacagaaa agcaagctaa
     1441 agatttaggc ataaatgtca aatctaaatt tatttctgat tataatcaaa ctcattatta
     1501 tccaggaaga aataaaatat atgtaaaact tatttatgat gcagatacaa aagttatttt
     1561 aggcggacaa gtggctgggt ttaaggatgc tgtccaaaga gcaaatgtat tggcagctgc
     1621 tatctttgga aaaatgacca caagtcaact tggaatgtta gatttatgtt atgccccacc
     1681 atttgcaaga acatgggatg ttttaaatgt agctggaaat gtgtctaaat aatatttaga
     1741 ttatagctaa tatagttgac aagcaattaa gggaatgaat ttacctaaac tatttttatc
     1801 atggtaaaat tcatccctaa atttttattc caaattttta gaaataagga ttccattaaa
     1861 ttattcatca aaatttaaac gcaagcttaa tctatcttct ccaagaatag tattttcaaa
     1921 aacattagta gcattttcta aatacatact aggtatgtct aaagaaggac taaaatgggt
     1981 cagtaaaagt tgtttgacat ttccaagttt tgcaagatta gcagcttctc taaaagtcat
     2041 atgtttgttt ctaacagctt ttgatatatc taaatcatca ccatacatgg cttcacatat
     2101 aaataaatca ctatctttta tgaaatctgg tattgattct ataggtcttg tatcggttgt
     2161 aagacttatc ttaactccct ttctcaattc tcctaagacc atatctggag tgtattgttt
     2221 tgaatttaaa actatatttt gaccttcttg gagtttgttc cataatattt taggaacttc
     2281 gtttttagtt gccttatcta tatcaaattt agggtttctt ctaaaattaa aactgtatcc
     2341 taaacactct atagagtgtt ctaaactgat tgttgaaatt tctaaatttc ttaaaacttt
     2401 attatctaaa gaaaaattgc cttgagggtt ttctataatt tttaaagtgt atggtacata
     2461 ttctactaaa tttctcatag aacgtataca atcgactatt ccaactggac caactatagt
     2521 taaatcactt gtacgaccac tattcccaat tgttgataaa agtcctaaaa gtccaaatat
     2581 atggtcacca tgtaaatggg ttatacaaat caagtctata tctttaaagc cacatttttt
     2641 tagcttcata gatacctgag taccttcacc acagtcaatt agtatttttc tacctttata
     2701 gtttataaat acagaggata gaaatctgtt tggcataggt acatttccac cacatccaag
     2761 taaagttaaa tctatcattt tttccccttt ctaaaattaa cacaaatctg ttgtttgttt
     2821 ttattatgat atcctattat aattatttta acattaaata taacaataaa tgtgtatttg
     2881 agtcaagaaa tattatcaaa taaagttttt atatagcaat gtataaaaat ttggagaacg
     2941 aaaagtgaaa attatattta ggagtattta aaggaggaaa atttaatgaa agtattaaag
     3001 aaaattacta ttttgtcatt gattacatta accttagttg gttgtagtaa cggagaagat
     3061 aaaaaagtgg ttgaaggttc taagaaaatt caaaattcta aaaaagatga acagcctata
     3121 atagtacata aagatgcaaa tatattgaaa gataggtatg agccaccaaa aggatttaaa
     3181 agagttgatg tagaaaaagg tagttttgaa tcatttctta ggaatactaa gctaaaaaat
     3241 tatggagaga aagtaaagta ttatgatgga agaattaagg aatcaaaagg tatttatgat
     3301 agtgtatttg atgttgatat tggcaataga gatttgcatc aatgtgcaga tgcgattatg
     3361 cttatgagag cagaatactt gtatcagaat aaactatatg atgaaataag ttttgatttt
     3421 gttgatggtt ttaaggcgaa gtattcaaaa tgggcacaag gatatagaat ttcagtaaaa
     3481 gatagtggtt caagctggta taaagcaact gaatattcaa catcttatga gagtttcaga
     3541 aagtttatgg atatagtgtt tgcatattca ggaacgttat ctcttgaaaa agaattggag
     3601 cctgtaaaag ttgaggatat gaaaatagga gatgtgttta tagttggtgg aagtccagga
     3661 catgcaagta taattatgga tatggcagaa aatgaaaaga ctggtaaaaa ggtatttatg
     3721 atagcccaaa gctatatgcc tgcacaacaa acgcaacttc ttattaatcg taaaaatcca
     3781 aatttgagtc cttggtatag tttagatttt gaaggtgatt tgacaatacc tcaatggact
     3841 tttaaaagag accagttgaa aagattttaa aataataatt ttatataata aaagaaaaat
     3901 atagatgtga aaaaatgtaa ttagattaaa actaattgga tatggacaaa attaaattga
     3961 tagataaata ttaaaaatta cactaatcaa tcttactata gtttaatatt tatctaaaaa
     4021 gttttatgta aaatataaat tttatattaa agctaaaact aatttgagga ggctaattta
     4081 tattgaagat tagaaggtat ttaattttgg taattataat tctaattaca acaagtcaga
     4141 gttttatttc ttatgggcta gagcgatttg aagaaataac catttttcac actaatgata
     4201 tacatggaag atatgctgaa ggtgatgacc atatacaaat aggaaatcta gccacattga
     4261 aaaaagaaac tccaaactca attttagtag atgcaggaga ttgtttgcat ggacttccaa
     4321 tagtaaatat ggatagaggg aaaagtgcaa ttgaattaat taaatgtgct ggatatgact
     4381 atattactcc tggaaatcat gactttaatt atggaaaaga tagactgttt gagttatcta
     4441 aaagtgctaa tttaggagag aatactttaa aatttttatc atcaaatatt tttgaaaatg
     4501 gaaaaaaagt ttttgatagt aatgatataa aagaaataga tggaataaaa ataggatttt
     4561 ttggactctc aacccaggaa acaaagaaca aaactggatt taaaaatgtg gaaggattag
     4621 agtttaaaaa tcctatagaa agtgcaacag aacaagtaaa tgaattaaaa ggaaaaggtg
     4681 ccaatgtaat agttgcaata tgtcatattg gtacaaatag tttaagtaag ccaaatagta
     4741 tagatatagc aaatgaggtt caaggaattg atttgattat tgatggacac agccacacta
     4801 agtttgaaaa tggtaaacaa gtgggtgata cattgattgt aagtgcagga caatatttag
     4861 aaaatattgg cgagataaaa ctaactttgg atactaatga tgtagaaaat ataaaaatag
     4921 aaaataaatc agttagatta attggaaaag aagaagcatt aaagtataag cttgactacg
     4981 ttgtaagtaa aaaggttgat gaaataaaag aacaacaaga aaaaatatta gataaagtta
     5041 taggtgcaac tacaaatact ctagatggtt catatgaaaa tgtaaggaca aaagaaacta
     5101 atttgggtaa tttaataagt gacattttac tagataaaac taaggctgat atttctttat
     5161 ttaatggagg gaatattcga gatacgatag aaaaaggcaa tataacaaga agagatattg
     5221 tagatgtatt tccttttagc aatactatag ttaccaaaga acttacagga gcacagatta
     5281 aagatgtatt ggagcatgga gtaaaacttt atccagaaaa aagttcagcc tttttgcaag
     5341 tgggaggaat ttcatactac tttaatccta aacaaaaaga aggcgaaaga ataacaagta
     5401 ttcaaaaaga aggaaagcca ttagatttaa ataaaaagta tgtagttgca acgaatgatt
     5461 atatagcctc aggtggagat gaatttcctt gtttttcaaa agagccaata ctaaaggaat
     5521 ttggaaattt agagagtgca gtcattgaat atatagagta caaaaaagaa atatcaaaaa
     5581 atgtagatgg aagaattggt attaaatcta aagatgagtt aataagcaag aatcatgaga
     5641 gtgatttgat attaagtgaa aatgaaaaaa atatcaatga taatgaagag aatctatcta
     5701 ataatataaa aagctctgat agtgacaaaa ataaaagctt agataaaaca agtgctagag
     5761 aaagttctaa tcaaggaata tctaaaagag aagctaaaga aaaatctcct aaaacaggag
     5821 atttaggttt ttcaaatagt ataattatat ttatagtttc aagtacattg atatgtttat
     5881 tgaattttaa tcaaaaagag ttaaaggata aaaagtcaaa ataaaaatta atgagcaaaa
     5941 ttagtagata gtaatttaat tttagataat agtaaacagc aaaggaaatt agttatataa
     6001 atactaattt cctttgtttt atgctttaac atgattattt ttaaataaaa ttgtatttta
     6061 tgaaaaataa ttacactaaa tggcatagta ttatggggat ttttagtttt tattcaagta
     6121 aatgaaatca tattaacaac ttctgatata tttacaaatt gataaaaaaa tgattttatt
     6181 agtatgatat aatttagcta gtatatatga tgatggagga tatagggatg aataatatat
     6241 tacttttgga agatgacaaa tctttaaata gaggaattag ttttaaatta aaaaaagaag
     6301 gatacaatgt ttttagtgca tttggtatag aagaagcaaa aaatattttc gcaaaagaag
     6361 agatatgctt aattatttcc gatataggtc tacctgatgg tagtggtttt gacttttgtg
     6421 aggagattag aaagaaaagt gatgtttaca taataatgtt gacagctctt gatgaagagg
     6481 tggatattgt cacaggatat gatttaggtg cagatgatta tatcacaaag ccttttagct
     6541 tgatggtatt aatatcaaaa gtgaatgcgc ttatgaaaag agtaaataca gtaaaaaatt
     6601 atactctatt agtttgtgat gatttattct tttattatat agaaaataaa ttaattataa
     6661 gaacagatga taaggaagaa gagataattt taagtaagac agagacaaaa ctattaaaat
     6721 atttgatgga aaattctatg cagacattga ctaaagaaca actgttagaa tctttatggg
     6781 atagtagtgg taattttgta gatgataata cgattgcagt aaatataaga agacttagac
     6841 aaaaggttga aaaaaatcca tctgaaccta aatatataaa aacagtaaga ggggttggat
     6901 atatgtgggg agaaaggagt ataaaaaaat gctaaaaaat atgaacctca aagatggttt
     6961 aagtgtgaaa gatgattata taatgagaag agttattgta tattctgttt tatctgtact
     7021 aatagtaacc cttgtattta tactttttga atatagcaga ttacataaat tgtatgaaca
     7081 tcaaataaaa aatactcaaa atattgctgg aatattgatt gaaaaatatc ctaatgatga
     7141 aatagatata gtaaattcta tttataattc cgattatagt aaggtgtcta ctggagaaaa
     7201 ggcatttaag aaatttggct atggattgga aaataaaatg tcaaatgata aaaattttag
     7261 tttatactta aaatcttttt ttaaggaatc attttttatt tttttaatga tgtttatagt
     7321 agttataggt ataatatatt attttataag atacataaat aaaagattga gtaaaattta
     7381 ttttatagta gataagatgg cacaagaaaa ctacttgaag gacactactg agtcagaaga
     7441 aactggttat ctagtagaat ataatgagta ttttaaagaa ggtacattta gtaagataaa
     7501 taactgtcta tatgagctta atagaagttt aaagataaag tttattaaat tagaaaaaga
     7561 aaaagaaagt gtaaaatcat tagtaacaga tatttctcat caattaaaaa ctcctttagc
     7621 atccttaaaa ttatacaata cattacttat agaagaaaaa ttagaagaag atgaagccaa
     7681 cgagttctta ttaaccaata aggcttctat aaataaatta gaaaatttaa taaattcttt
     7741 agtgaacata tcaaggttag aaattagcat gataaacata aaaaaagaag ataatgatat
     7801 aaaaaatact attctaaatg cagtaaaaag tgttacccct aaggctaaga gcaaaagtat
     7861 aacattaaat ttaaatgaat ttaacagtag aattatacca catgataaaa agtggactga
     7921 ggagagtatt tttaatgtat tagataatgc tgtaaagtac acacaggtaa atggagaaat
     7981 aaatatatct gtagaagaaa ctgctaattt ctttaaaatt ataatagagg ataatggcat
     8041 agggattgaa aaaaatgagt ttaataatat atttaaacgt ttttatagag gttctgctga
     8101 tgaaattgaa tctgtagaag gtagtggggt aggattgtat ttaagtagaa agattttaga
     8161 agaacaaggt ggaaatatta tagtttcttc aaaagtaggg atagggagta agttttcttt
     8221 gtttcttaca actatgtaat attaaatgaa aggaaaatgt aagcctctac atttattata
     8281 taagtataaa cagctccaga aataaaaaat taataaaata tggggtaata taacttagaa
     8341 tgaggggata attttatgaa aatactatat actgaaaatt tatcaaagca ttatggaaaa
     8401 ggagaaaact tagtaagagc tttggacaat gttaacttag aaataaatga aggtgaattt
     8461 gttgcaataa taggaaaatc aggttctgga aaaagtacac ttttacatat gattggaggt
     8521 cttgatatac ctacatctgg gaaggtgtat atagataata aaaatatatt tacattaaaa
     8581 gaagaagaac tagctgtgtt tagaagaaga aaaatagggt ttatatttca aagctataat
     8641 ttaattccat ctcttaatgt atgggaaaat gtagtgttgc caatcggtct agatggtaga
     8701 gaagtagatg aatcttttat aaaagaactt ttgaaatcat taggacttga aaataaacat
     8761 gacgttttac caaatacatt atcaggtggt caacaacaaa gggtggcaat agcaagagca
     8821 ttagctacaa gaccagctat aatacttgct gatgaaccaa ctggaaacct agattctaaa
     8881 acatctgatg aggttatgag tatattaaaa tctatgtcta agaagtacag tcaaactcta
     8941 gttatgataa ctcatgatga cagtatagcc caaatggctg atagagttat attcatagaa
     9001 gatggcaggg tgtcaaaggt tggtgataaa aatgattaat actactaaaa tagcaaaaag
     9061 taatttaaaa caaaataaat caaaaagtat tctaataata atcactataa tactttctac
     9121 tttaatgctt tcttctattg gaatttatat tgttgatgca ggagcatatc aaaaagaaaa
     9181 tactataaag tattcaggta attatcaagg tatattagca aatgtagatg aaaaacaggc
     9241 tgatatattg agtaatcatg cagatgttga attaactggt gaaatgaatg gagtaggtgt
     9301 tgaaaaactg gaagatgatt caaatatttc tctagcatac atgaatgaag atgctttgaa
     9361 attaaatagt tttgaactta tcaaaggaaa attgccaaca aaagagaatg aaattgtgtt
     9421 agatagtgga gctttaaagg cacttggtta taaaaataaa gacttaggtg aaaagataaa
     9481 aatatcttat aatgactata aaaatgataa aaaaattgaa aaagagttcg ttataagtgg
     9541 aatcttaaaa actagcgaaa tatctgaagc aggtaaatat tattatgcta ttatatcaga
     9601 attatatatg agaaatacta gagatatgtc acaagaagat tttaatattt atgtaaaatt
     9661 taatgataaa agtaatttat caatagagca agcaaaagaa aaattagata aaattgctaa
     9721 tgatatagga cttgatacta ttaatactgc tgtaaatgaa aattatataa atgctttaaa
     9781 accagatatg gaaacgatta tgggtggagg ttttgtagga ttagtaattg tattatctag
     9841 tattttagta atatacaata tattttatat ttcaatagtt acaaaagttc aagaatttgg
     9901 aaaacttaga gcaattggtg caactaagaa acaaataaaa aatatagtgt ttaaggaagg
     9961 gtttatatta gccggaatag caataccaat aggaataatt ttaggctatg tacttgcaaa
    10021 tattataata aaatcattta tggatataga tgtaaaatca tcacgattac cagttatatt
    10081 atcggttgtg gtaataagct ttatatctgt tgttttatca cttttaaaac caatgaaagt
    10141 agcatcaaaa gtatccattg ttgacgcagt aagatataca ggaaataaaa taagtaataa
    10201 gagtaagaga aaaggatata aaaatataaa tctaaagaga ttatcacatg ccaatttaga
    10261 aagaaataaa aaacgtactt acatgacatt ggcatcactt atacttagtg gaactatatt
    10321 tataacagta tcaactgctt tagaaagttt tgatgctgag aaaatggcaa gagaacactt
    10381 tccttatgat atagaagtta gactatctgg ttatgaaatg aacagtgata aaaatcctaa
    10441 aaataatttg aatattttac aaatggataa tcctctaagt aaagattttt ttaatcaaat
    10501 aaagaacatg gaaggaatta aaagaataga atctgcaaga agtgtaaaaa ttggtatgga
    10561 ggactatgat gttgaattta agtatgacct tttacaaagt ataaatgaaa atgatgtaaa
    10621 atcattaaac aagaatctaa tagatggaaa aataaatctt gaaagactgc aaactggaga
    10681 tgaaatagta attactcatg ttgatacagc aaaagaaatg ggagtaaaag caggagataa
    10741 aataagatta actttatatg atggagataa aaagataaaa aaagaattta aagttcaagc
    10801 aatagctatg ggggttccaa gttttggtat agggaaggat tttattgata gaactttaaa
    10861 atacgattca actagtagct tgggaatata tacagaagaa ggaaagtatc aagaaatcaa
    10921 agatagtatc aagaagattg ctaaaaataa tggattttta gagactgatt ttatagatag
    10981 tagaattgag tcaaataagg ctgttatttc atttataaag ataatgggat acacgctaac
    11041 aggcataata ggtgtgattg gatttatgaa tcttgtcaac acaatgataa ctagcatagt
    11101 tactagaaaa aaagaacttg gtatgcttca agctattggt cttactaata aacaattagt
    11161 gaaaatgctt aattcagagg caatgtatta cacttctgga atgatgatag gaagtatttt
    11221 atttggaggt atattagggt atattgctgt tatatttcta aaaaagactg gattatctta
    11281 tgcaacatat tctttgccaa tagtaccaat cttgttgatg attgtgtgta ttcttatagc
    11341 tcaatttata acaacttatt tgataggacg aagttttaat aaagaatctc ttattgatag
    11401 agtaagatac agtgaataaa atgcaaatat ttgtggttag tttaactagc aaaaagcata
    11461 taaaaaatat agttaaagag attcataatt aaataggtta aagcatagta cagagcaaac
    11521 aatttaaacg gatgtttgct ctgtatttta ttatgtaatc aataatatat ctaatatact
    11581 gatattattc atgttagaaa atgtatcggt acaattctaa aaaagtataa aattgtacca
    11641 tataaaatga aaattttgta taataatcgt atagaaaata aataggagct gatatatatg
    11701 aataagaaga ttaattatgc accaggacca actgaaacta gggaaaatgt aagattagca
    11761 agagcagaaa aaacaacaaa tccagatata gatattgatt ttgttgaatt ttataaaaag
    11821 acatgcgaga agtttggcaa tatagttgga acaaaaaacg atgtttacat attgagtggt
    11881 gaaggaatat tagggcttga agcagcatgt gcatcactta ccgaaaaagg agatagagta
    11941 cttgtaattg acaatggtat atatggagaa ggttttaaag attttgtaac aatgtatggt
    12001 ggggagtatg tgcttttttc aagtgaatat actaaaagta tagatattga tgaattgaaa
    12061 gcatttttag atgaggatag taattttaaa tatgcaacag tagtgcattg tgatactcca
    12121 actggcgtat taaatgatgt aagcaaaata tgtccattac ttaaagaata tggaatatta
    12181 actgtagttg attctgtagc aggtatggta ggagaaagat taagtgtgga tgaatctaaa
    12241 atagatataa tattaggtgg ttcacaaaaa gctatttctg caccagcagg acttactata
    12301 gttggaataa gtcaagatgc aaaaaattgt ataaaaaata gaaaaactga tgtaataggt
    12361 ttttattgta atttaaatat atgggaaggc tattatgaaa aaaaatactt cccttataca
    12421 atgccaataa gtgacataat ggggcttgat aaagcacttg ataatatatt agaagaagga
    12481 atagaaaatg tattagatag acatgaaaag atagcttata gtgttagaaa atctattgag
    12541 gaatatggtc tagaactatt tttagaagaa ggatattcta atacagtcac agctataaaa
    12601 attcctgaaa gtataggagc tttaaaactt actgattata tgcttaaaaa ttataataca
    12661 cttgttgcaa catcactaaa tcaatatatg gatactattt taagaatagg tcatatggga
    12721 gagaatgcaa atctaaataa aatagagcat gtactaaatg tcttggataa aagtttaagt
    12781 gcattaggat tcaaaggaaa tgaaaatttg ttgaatttat ttaataagta ttatttttaa
    12841 tagatagggg gatatagtat ggaagttaca gctaagcaaa aatttacaac gaaagacatg
    12901 gtggaaacct cactattaat agcattagta tttattgcaa ctaaatttat aaatataaga
    12961 ttaccaatat ctataaatgg tgggcttgtg catttaggaa cagcaatgtt gttcatatca
    13021 gcaatagtat ttggaagtaa aaaaggtgct ttatcaggag ctattggtat gtcacttttt
    13081 gatttaatat ctgggtggac tttatgggca ccgtttacct ttatagttag aggagttatg
    13141 ggttatttac ttggaaagat tgcatgggca aatggtaata atgggaataa ttttttaatc
    13201 aatgtaatag gaatatgtgt ctcatcaatt tggatgttat ttggatatta tataacagaa
    13261 gttatacttt atggtaattt tatagtacct cttacttcaa taccaggtaa cttaatgcaa
    13321 gtattgatag gtcttataat agcgcttcct atttctaagg tattgaaaaa atgtataaaa
    13381 taaatataaa gtgaactaaa atttatgata gcataaatta tactgtataa tattaagatg
    13441 gagtacataa ttaaaaaatt ttagtataga ggttagttca caatgttatc agttacaaga
    13501 tattcaatca aaaatgttcg tttaaagccg tgggtaaagt ttatttggta ttttgaaaca
    13561 aaatcaaata tactgttaaa taataagtta cttcctacag acagtataga tattattctt
    13621 aatctttctg atgttatgga atataaaatt gaaaatcaag actatactgc tagtaatatg
    13681 cattttaatg gaataaggga taaacatgga tttattattc aacatggaaa tatacgtgtt
    13741 atagggattt ctttttatcc atttggtctc tatccatttt taaagatacc aatttcagag
    13801 tttaacagac aaattgttga cttagaagca gtatcagaac tatttgccaa aaaattggaa
    13861 gagtcattaa atccaacaca atcagtagaa aaaattgttt tatatttaga agaaatgtta
    13921 ttatccatat tagaagaaga tttaatttca aataaatctg taaagcttct aaattcattt
    13981 atatatgata atagatatag caatataaaa gcattttgtg ataatacaaa tataaatatt
    14041 aaaacattgg aaagaatctg tttaaaatat acagggtata cacctaagat tttaaagaga
    14101 atttacagat ttaaaatggc aagtagtcaa cttatttata attataaaga taatgaactt
    14161 tttgatttta tttatgaaaa tgagtactat gaccaagcac actttataaa agaatttaaa
    14221 aaattttcag ggacgtcacc aattaaattt attggtgaga ataagacgat taaagaaaat
    14281 ataaagtaca actatttata attatactgt cgatttttta caatacctcc aatttatttt
    14341 ttgatatgat ataggaaaat aaattggagg ttttacatat gaaaattatt acagaaatag
    14401 ttgagtttaa tgtggaagaa aatttatcaa aagaagagtt tatagaaatt gtaaatgact
    14461 tagaattaaa atttcattct attcaatctg gttttattga tactgagctt ttatatgatg
    14521 aaaagtctca aaagtggatt atgatacaac attgggaatc tatagaagac ctcaagagtg
    14581 cttctagtaa aatgtttaaa gattcttcaa ctgagaaatt tagaaaagct ttaaagaatc
    14641 aaactgttaa gatgaatata ataccacaaa ttaaaagttg gaaattatag aactgaatgt
    14701 aaaagatagt taaatagtat caaactaaat tagccaaata acattttgta tttaaattgt
    14761 tatttggctt ttagtgttta ctttaatatg agtgttatat actagaaaaa ccaacaaact
    14821 gttagagtgt ggattataat aatgtcatat ttgggagcta aaaggcttaa tactgatttg
    14881 tcaatatgtc tttttagcat gttgaacagg tatattgcca tgctttcatt attttatttt
    14941 tttgccttat actggactac atttgcagtt tcctaatata tttggctcat atttaaatgc
    15001 cattatctaa aagtactatt gctgtacgag aggcaatagg cattttagaa gaaggaggag
    15061 ttgagctaaa gtatacatca taatctctta agttttcttt ttttgtgttt cctccaaact
    15121 ctaccatatc agtatttaat atcgttttgt agtttgtatt ttgaaacttt gaaatatcaa
    15181 ctgattgtat tagattaggg tggaaattaa atataaataa atatttgccc ttactgtaca
    15241 ccaaaatctt tttatcatta tccatatgta taagttcatg actattttgg ataaaaatat
    15301 cactgtattt tgtaagttcg agcatagatt tatcaaaatt taataattgt ttatacttta
    15361 agttgttatc ttctgaaagg ctccattgtc ttctggcata cttatagctc caattgttgc
    15421 cttctcgtgg aaaatcaatc cactcaggat gtccaaattc atttcccata aaatttagat
    15481 atccttcacc tgctagagaa aaagttatta atttaattaa tttaataaga gatattgctc
    15541 tattgataac atggttattt gagtttattt ccatattcca gtacatttcc ttgtcagcta
    15601 gccaaaaaat aattgtttta tcgccaacta atgcttggtc atgggattca caatatccta
    15661 tatttttttc tccaggtcgt ctagtagtaa gttcatacca catttttccc aaatcccagt
    15721 cttcatcaga taagttagag atagttttta tccaaaaatc aggtactccc attgcgagtc
    15781 tatagtcaaa tccaattccg ccgtctttta tagggataca cataccaggc attccactca
    15841 tatcttctgc aatacttatc gaattaggtt taatttcttt tattaattca tttgcaaatt
    15901 ggagatatgt tatagcttca atatctgtat tcatactaaa atatttttca taactatcaa
    15961 atgaaactcc aagaccatgg ttatgatata acattgaagt cacaccatca aacctaaagc
    16021 catcaaaatg gtactcattt aaccaaaatt ttatatttga aagcaaaaaa tgtattactt
    16081 caggtttacc atagttaaaa agttttgtac cccaagcagg atgatttccc tttgaacctg
    16141 agtggaaaaa ttgatgttca cttccatcaa actcatttat tccttctaaa gtattcttta
    16201 cagcatgaga atgaactaaa tccaataaaa cagcaatccc cattgaatga gcagtgttta
    16261 tgaggttttt taagtcttca ggagtaccaa acctagatga gattgcataa aagtttgaaa
    16321 cttggtatcc aaaagatgca taatatggat gttccataat agccataagc tgaatggtat
    16381 tgtatccagc tttttttatc ttaggaagta ttttttcagt aaattcatta taagttccaa
    16441 tagattcgct ttcagtagac atacctatat gacattcata gattagtgga gaagttatgt
    16501 tttttaagtc aaaattattg tcagtccaga taaatggagt ttttggttgc catatttgtc
    16561 cattaaatcc accaaaatct ttttgaatta ctctttttat atataatggt atacggtcaa
    16621 atgtttttcc atttgcagta acttgtacct ttacctctga tttgtgaggt aagctatttt
    16681 gaccaggaat aaatatttcc caattaccag acccaatttt tcttagagga tgagattttc
    16741 tattccaatt attaaagtca ccaattagtg acaaactatc tgcatttgga gcccattccc
    16801 tatagaagaa tccatcattt gtctgatgaa atccatagta aagatgagca tttgcaaaag
    16861 aactaaaatc tttatagttt ttcaatgttt ttgatttcat atcctcataa tgtttcattc
    16921 gcttttttat atcagattca aatggttgta ggtatacatc aatatctaaa attttaaatc
    16981 ttttttgact tttcattgta tttctcctcc ataataaata ttacatttaa aaaataatat
    17041 ttatctcata ctatcttctc tgtataattg atatatatta tataatccta ataaaaagct
    17101 taagccacca atgattaatt tcataattta cctccctagt atttatactt atatatcgta
    17161 ttcatcaaaa tttaaaagtt tgtttaaaac tcttttaata atatcataat caaagccttt
    17221 ataggaaaga tgttgagaaa ttttttgata tattttcttt ttatcttctt tctttactct
    17281 ttcatacctt ttttttgcta gatacatagc attttcaaat tcagtgtcct tgtctaattc
    17341 agatactgct tcattgattg tacttctatt gataccttta ttatataaat tctgcttaat
    17401 tctgtttttg ccacatttat ttaggttaac atttgtgtta acaatttttt gagctaatac
    17461 actgtcattt acgagattat aacttttaag aaaatctatg accctctcaa ttatattttc
    17521 atcaaattct gatgatagtt tttcttttaa ttttttttca gattggtcag catgagataa
    17581 aatatttaat gctttatttt tagcttttat atacatttca tcatctaaaa taggttttaa
    17641 ttcttcttca tttatttcat tccctttttt taaattaaat gtataaacaa gttctttaaa
    17701 tatagcaata aaaaatttat catctacata
//
LOCUS       DAGQWR010000086        17186 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.086, whole genome shotgun sequence.
ACCESSION   DAGQWR010000086 DAGQWR010000000
VERSION     DAGQWR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17186
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.086"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            269..406
                     /locus_tag="MIW95_002494"
     CDS             269..406
                     /locus_tag="MIW95_002494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151814.1"
                     /translation="MGDSDCSKMLAFYEIMKMLETIDETKFYNCKEEIKSEFKHIKKE
                     D"
     gene            complement(433..825)
                     /locus_tag="MIW95_002495"
     CDS             complement(433..825)
                     /locus_tag="MIW95_002495"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3151815.1"
                     /translation="MYKDESNIFSKRLREEREELGLMQKEMANKLSLPANTYNGYETG
                     KRSPALDVVRHIADTLDISTDYLLGRTNIKINISNITEKELIKKLNPSDDMKEILDIF
                     SELDDDSKNKALKIAKLFLEEQNTKNKE"
     gene            1014..1208
                     /locus_tag="MIW95_002496"
     CDS             1014..1208
                     /locus_tag="MIW95_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008422227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3151816.1"
                     /translation="MKNYILKSLRVRQGLRQRDLAKILGMNPSTYSSKENGDRRFTVS
                     EAIKISDFFNTDLRDIFLNK"
     gene            1244..2035
                     /locus_tag="MIW95_002497"
     CDS             1244..2035
                     /locus_tag="MIW95_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBY3151817.1"
                     /translation="MNNLQVIEKNNERVLTTQQLADVYETDARNISNNFNNNKDRFIE
                     GKHYFLLQGDDLKHFKGIHTEYENLKFTSKMYLWTERGANRHCKILDTDKAWEQFDNL
                     EETYFKVKQHKPSCIEDVLIESLKEMKDLRLQVNRANNIALEAKAEVETIKDVVSLDS
                     NSWRTDTHQLIARIAKKQGGFEHINTLRTESYELLNKRFGVDLHRRLINKRRKMAEEG
                     VSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            2127..2319
                     /locus_tag="MIW95_002498"
                     /pseudo
     CDS             2127..2319
                     /locus_tag="MIW95_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056356.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2323..2520
                     /locus_tag="MIW95_002499"
     CDS             2323..2520
                     /locus_tag="MIW95_002499"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151818.1"
                     /translation="MKISFESVVNNVNNETELILSKEELQIAKRILNILNENEQSILS
                     SKDILDFCKEALKYNLVPTFV"
     gene            complement(2507..2689)
                     /locus_tag="MIW95_002500"
     CDS             complement(2507..2689)
                     /locus_tag="MIW95_002500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151819.1"
                     /translation="MQFSKDILHTLTLEILKKKYDFKNSSEEELLKHYHEIFLKLSEV
                     NNSFSKGDGLSVFKQT"
     gene            2760..2957
                     /locus_tag="MIW95_002501"
     CDS             2760..2957
                     /locus_tag="MIW95_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3151820.1"
                     /translation="MAINDNINKILRDRNIKAWKLAKEIGVDSGNLYSILRGKNKNPT
                     IDTLIKLADYLDVTLDELVGR"
     gene            2996..3205
                     /locus_tag="MIW95_002502"
     CDS             2996..3205
                     /locus_tag="MIW95_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151821.1"
                     /translation="MTKVVAKEQLFYRAKDVAKFLDICEATAYKIIAELNEELEKEGF
                     KTFSGRVSIAYFKERYCYKPRKGVI"
     gene            3307..3495
                     /locus_tag="MIW95_002503"
     CDS             3307..3495
                     /locus_tag="MIW95_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151822.1"
                     /translation="MRIIYKNKIYKVEQDKVLFRITYYDEQKNNRKFNNNKKVKRSVL
                     TRDIEVVNLYLPTHLKIK"
     gene            complement(3630..3809)
                     /locus_tag="MIW95_002504"
     CDS             complement(3630..3809)
                     /locus_tag="MIW95_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151823.1"
                     /translation="MINMSNKDIYTREEDKRFTLRINKLLFEKIEQLAQKDKRSVGRE
                     IEFILEKYFEDNPLE"
     gene            3940..4092
                     /locus_tag="MIW95_002505"
     CDS             3940..4092
                     /locus_tag="MIW95_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBY3151824.1"
                     /translation="MYDKDERVRFTFRLPATLLEKMKDIASKEGSSMNSLILHILWDY
                     IKEIEK"
     gene            4103..4318
                     /locus_tag="MIW95_002506"
     CDS             4103..4318
                     /locus_tag="MIW95_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151825.1"
                     /translation="MNKFDLKKVEVGNAVKVNCKRFGFEIDCIVVVATENELNLAYYD
                     KERGCMEYQALIVEDVRYGDYEILFLN"
     gene            4331..4501
                     /locus_tag="MIW95_002507"
     CDS             4331..4501
                     /locus_tag="MIW95_002507"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151826.1"
                     /translation="MELAISVATSQIKQAKKNIKELKAKEDYDKDTLSWWEGVKQASE
                     NILEFLEIENKA"
     gene            4585..6537
                     /locus_tag="MIW95_002508"
     CDS             4585..6537
                     /locus_tag="MIW95_002508"
                     /inference="COORDINATES: protein motif:HMM:NF024867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY3151827.1"
                     /translation="MKAILLKSLNIENFKGVKELHIDFNNVTNVFGENATGKTSIFDA
                     FTWVMFDKDSKNRSVFEIKPLDKQNKVIRGLVTTVTAVLEVNNKEIKLTKKYEEKWTR
                     KRGESEATFTKNETTYMINDTPIKKSEYVKDIAEIADEDQFKLLTNPYYFSNELNWKK
                     AREVILEICGDITTEQIIEAKQELTPLITEFEKENNIDKIIKNRKATKNNLSKEKEEI
                     PVRINECDKSMYSIDFEEIETQLNAKKVSLEVIEDELLSGTKANEQILKDKEKVFELK
                     QEIQSIKQSASERGNKKRNELINEKEGLKYNVKTLRNNLVYLEESRKLKENSRNKLVE
                     ETNNLRNEWSKKSQEVLDLNTIQTECPTCKRPLETSDIEVKKQEILDNFNLNKANVLK
                     EIGVLGKSKGEEVKKLNTEIEQLQLDEKEALKEIQEKAVLIDKIKKELENTKSTEVYS
                     LDEENRLVEIDAEIKELEEKINNKDDNKNIDELKENKKKLAVEIELLNKELAKRDINK
                     ELLDRKEQLLEKEKALGIELAHQEKILNLCELFIKTKVSLLESNISSKFKNVTFKLFK
                     EQINGGIEETCEALINGVPFSNVNTAGQINGGLDIINTLSNHFGVKMPIFIDNRESVN
                     TLIDIDSQVINLVVSNDNPLKIEGVN"
     gene            6540..7409
                     /locus_tag="MIW95_002509"
     CDS             6540..7409
                     /locus_tag="MIW95_002509"
                     /inference="COORDINATES: protein motif:HMM:TIGR00616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBY3151828.1"
                     /translation="MANEIQRQVSVKNLLSTEAYKKRFKEVLKDKANTFMASVVNVSN
                     LPSLKDAEPNSILKSAMVAATLDLPIDPNLGFSYLVPFTNKGVKEAQFQIGYKGFIQL
                     AMRTGQYKTINAIEIYEGEIKSVNRLTGEIEFNKNEDEIDKEIVVGYIAYFKLLNGFE
                     KTLYMSKEDMEKYAKRYSQTYKSNKDYVVKSSLWTTDFDAMAVKTVLKRLLSKYGILS
                     IEMQKALETDQAVIKDDNSVEYVDRQVEEEIEENANKKTIDMTTEEKEKAIEIKESKP
                     EEVKQQEFEAPPF"
     gene            7409..8122
                     /locus_tag="MIW95_002510"
     CDS             7409..8122
                     /locus_tag="MIW95_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY3151829.1"
                     /translation="MKIKVLGSSSRGNCYLLQLKKETLILECGINYKDILKGLDFNLE
                     SVIGCLVTHEHKDHSKAITELTNNGIDVYSSKGTLEVLGIENHRTKVIESEKLFKIGN
                     FNIMPFSTKHDAVEPLGFLINHEEIGNLLFITDSYYCEYNFKNLNHIMIECNYSKDLL
                     DNNKNKIYLRNRIVKSHFELSNVINFLKANDLSNIKTITLLHLSEDNSDKDLFIKEIE
                     KNVGIPVIVAEKGLEIYLD"
     gene            8808..8939
                     /locus_tag="MIW95_002511"
                     /pseudo
     CDS             8808..8939
                     /locus_tag="MIW95_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            8986..9384
                     /locus_tag="MIW95_002512"
     CDS             8986..9384
                     /locus_tag="MIW95_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151830.1"
                     /translation="MELKFREWNKNGKEMYSYDEMVCYSKNLLREWVYSGVYLPTGNE
                     NSEVMIYTGLKDCIKKEIYEGDIVSYILSFEEFIGEVKFEEGFFVIDNEVLGECVGLF
                     HEIAVVKVIGNIYENPEMLEKIRKPKVLEG"
     gene            9387..9638
                     /locus_tag="MIW95_002513"
     CDS             9387..9638
                     /locus_tag="MIW95_002513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151831.1"
                     /translation="MRFEIGKTYKFDKEKFIEINGVEHYKKYKELWIDNIEGVEFTVK
                     KIFENGYVHYPNEFYFAFGIVPEWCVEVKEVRGIRDDNN"
     gene            9625..9876
                     /locus_tag="MIW95_002514"
     CDS             9625..9876
                     /locus_tag="MIW95_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151832.1"
                     /translation="MIIIRSQDRLDLTRVNRVEISNNRVYAMFEDGIRKIGEYESNER
                     AIQVLNEIQKFIESGVKMDYIDSCRVRHNQEKVFEMPVK"
     gene            9893..10243
                     /locus_tag="MIW95_002515"
     CDS             9893..10243
                     /locus_tag="MIW95_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151833.1"
                     /translation="MAKVWVDAGTFLERTKDIGDIECNLREMINKNKQKNLIGRINSK
                     GQGRKSKKVECTNIITGESKIFVSAVEASKYLYFTELHITRLARTGKTTKNGWKVRYI
                     QEVTDGISKCGASN"
     gene            10212..10349
                     /locus_tag="MIW95_002516"
     CDS             10212..10349
                     /locus_tag="MIW95_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151834.1"
                     /translation="MVLANVEQVIKLAEKIINKKKCSVNKAIDIAIKILSRYEYEGMI
                     K"
     gene            10346..10804
                     /locus_tag="MIW95_002517"
     CDS             10346..10804
                     /locus_tag="MIW95_002517"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151835.1"
                     /translation="MSLIEYRGYDFENKKWIYSKTIMWSNAVDCLLMLKEDFKWRKVC
                     NVGICSEEWARNNQEIYEGDILKEYDNFNDTSQYGVVKRDVGSIKIYLEWHYLKKFEG
                     EWIEIVNKTEIYHSRDYKVVGNEYENLEEVRKELLERKEKLENENTNAVN"
     gene            10779..10937
                     /locus_tag="MIW95_002518"
     CDS             10779..10937
                     /locus_tag="MIW95_002518"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151836.1"
                     /translation="MKIQMQLTKEKEFFKVYVNSEEEELEKLFYEFVSQMLACKRKNK
                     KVQGDIEK"
     gene            10934..11107
                     /locus_tag="MIW95_002519"
     CDS             10934..11107
                     /locus_tag="MIW95_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HBY3151837.1"
                     /translation="MKLKDIIKLGEKYCYCPSCGNDKIGNNEGKLIVEEHTYYRECKC
                     GFNILIDDRKDEI"
     gene            11226..11384
                     /locus_tag="MIW95_002520"
     CDS             11226..11384
                     /locus_tag="MIW95_002520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151838.1"
                     /translation="MDDRLEICKKMFPNVTKNTRLLCHYCDGRNICSYDEEKARKLLR
                     EKEEVKND"
     gene            11377..11559
                     /locus_tag="MIW95_002521"
     CDS             11377..11559
                     /locus_tag="MIW95_002521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151839.1"
                     /translation="MTNFEMIKNMSKKELAEFINEIGDNCTCIYCSCQYRYEICDVDE
                     KCVDGCKEWLDMEVEL"
     gene            11725..11946
                     /locus_tag="MIW95_002522"
     CDS             11725..11946
                     /locus_tag="MIW95_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151840.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSKEMLKQKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMAEYFEDEGVEL"
     gene            11943..12941
                     /locus_tag="MIW95_002523"
     CDS             11943..12941
                     /locus_tag="MIW95_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBY3151841.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDMDIFFQKKINKVLR
                     DNDIRKAVFNDYSSNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYTEKDEKKVAILKLDYNKSYTHSISFEDDKFNIQMSKNEINIQETKAIKIGAIIG
                     LSGMNDEYHLKVLDKDAEKEEANSKFVTGFLNATKIKDDKFKTKMFKGLVDAFIANLY
                     SDMKQGEDVRNILLYMLREKQKLDINEFTDKAIKGDLKDSFKDHVEEKGLDGSFNIDK
                     KWVEKNLKNRHIKTDTGIEIKGKIDDFEDFMKYGIRHNGNGTVDIIIKNVSFYNEK"
     gene            12952..13242
                     /locus_tag="MIW95_002524"
     CDS             12952..13242
                     /locus_tag="MIW95_002524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151842.1"
                     /translation="MKEQGRLVLNKLGTVDEVMKKVETDEKEINVYLKELKECEKAKV
                     LIIGKDGTEIPIVFKDETMNEWLRKEIEISFDEFKDEIVQVKQVLLKITGDN"
     gene            13262..13429
                     /locus_tag="MIW95_002525"
     CDS             13262..13429
                     /locus_tag="MIW95_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151843.1"
                     /translation="MTNKEMCKSKNITEKESYKRFSKEICKSCKNNKRDCESKDCDIT
                     YKNWLEDIYYV"
     gene            13422..13793
                     /locus_tag="MIW95_002526"
     CDS             13422..13793
                     /locus_tag="MIW95_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151844.1"
                     /translation="MYSTYKVKLKTKRTLEQVRNQSVDFEYSEEGLKNTLKYYNLIDS
                     LEVIVVKFGDEYCLVDCDEEDEKTIMEAYYLLEQDEYTGCYINEYELFKKDWENGSYY
                     GEVCMVFSDDEIEIIEKLREG"
     gene            13953..14294
                     /locus_tag="MIW95_002527"
     CDS             13953..14294
                     /locus_tag="MIW95_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HBY3151845.1"
                     /translation="MANIYCENYNCENYFEDMCMLERIEINNLKVCESYIEGKNELYK
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEALEEELIK
                     INSHLELEDLF"
     gene            14419..14826
                     /locus_tag="MIW95_002528"
     CDS             14419..14826
                     /locus_tag="MIW95_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBY3151846.1"
                     /translation="MKVNFTIDGEPIGKERPRFNLSTKRTYTPNKTRDYEDLIKWLYQ
                     SKVKHYFEGYIKMTLRCYYSIAKSNSKKVKEQKKNNVLRPSKKPDIDNVVKIVADALN
                     EIAYKDDTQIVEVVASKYYSDRPRIEVILEDVI"
     gene            14928..15782
                     /locus_tag="MIW95_002529"
     CDS             14928..15782
                     /locus_tag="MIW95_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HBY3151847.1"
                     /translation="MNENINKEITVLGTLEVEGMKFHDIEGGFGKGKKAMLVKDIAEI
                     HNRELKHINELINNNIKRFKTNIDIIDLKVSRSERLGDLNANPFEGLGYEAVGYSKQS
                     FNQSRNVYLLSERGYSKLLKILEDDVAWEQYEKIVDGYFSMRKELNNPLLSASKELQA
                     IFMLDKKQEVLETKIENVNEKLENFIDDAPLFNIECECIVKEVKRVATKSLGGHGSKA
                     YKNKSLRGKVYSDIYHQIKREFGVDSYKAIKRCQLNKVLEIVNSYKLPIVFEEEIRLL
                     NSQLSIVS"
     gene            15886..16392
                     /locus_tag="MIW95_002530"
     CDS             15886..16392
                     /locus_tag="MIW95_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151848.1"
                     /translation="MSKTKKEFFNATKKQLSNYKQLNANIIKLKNEIQMLKDNSVGDL
                     TKAISYDSVKTGKTNKTSNMIEDAIVNVSDLITEKEIELYEAEIIKSTIDLAIRNLKP
                     IHRQIIELKYIDGLMWQEMVDIIHLEERQLSVRASQAISSISIALFGKKALIEQEPLF
                     ELLDYKLN"
BASE COUNT         7377 a         1581 c         3175 g         5053 t
ORIGIN      
        1 aaaatctcta tattagtcaa gttgcaagat aatataaatt tatatataat atatttacaa
       61 agaaaatctt agaaatttta aatattatat ttgttgcaat aaatatatca agtctataag
      121 ttaaggaatt tttataccat tttttacgaa cataagttct gaaaagggat ataaaatata
      181 atattataaa aagaaggtga ttggctaaat tttttacata tttaaggggc aaggaataaa
      241 tattataaat tataagaggg tgacgcaaat gggagattca gattgttcta aaatgttggc
      301 attttatgaa attatgaaaa tgttagagac tattgatgaa actaaatttt ataattgtaa
      361 agaagaaata aagtcagagt ttaagcatat aaaaaaagag gattgaatcc tcttttttat
      421 atatttttat gcttattctt tatttttagt attttgctcc tccaaaaaaa gttttgctat
      481 ctttaaagct ttatttttag aatcatcatc aagttcacta aaaatatcaa gtatttcttt
      541 catgtcatca gatggattta gctttttaat aagttctttt tctgtgatat tagaaatatt
      601 aattttgata ttagttctac ctaataaata gtcagttgat atatctaaag tatcagctat
      661 atgtctcaca acatcaagcg ctgggcttct tttaccagtt tcatatccgt tataagtgtt
      721 tgctggtaaa cttaatttat tagccatctc tttttgcatt aatccgagtt cttctctctc
      781 ttctcttaat cttttagaaa atatattgct ttcatcttta tacattgtaa ttgtccccct
      841 cttaaaaaac tataacgaat gtaattactc ttattctact acaaaatacg cattttgtga
      901 ttttaattcg caaaaaggaa gaaaaaaaca aaaaacgtat tgacaattca caaaacggag
      961 tatattatat aaatatagaa acttcaaatt gagaataaaa ggagtgatat atattgaaaa
     1021 attatatatt gaaatctctt agagtaagac aaggattgag acaaagagat ttagcaaaaa
     1081 tattaggaat gaatcccagc acttactcaa gtaaagaaaa tggcgatagg cgattcacag
     1141 taagtgaagc aataaaaata tcagattttt ttaatacaga tttgagagat atttttttaa
     1201 ataaataatt cgcaaaatgt gaataaaaag gaggtagata aacatgaata acttacaagt
     1261 aatagaaaaa aataacgaaa gagttttaac tacacagcaa ctagcagatg tatatgaaac
     1321 agatgcaaga aatataagca acaattttaa caataacaaa gatagattta ttgaaggtaa
     1381 acattatttt ttattgcaag gtgatgattt aaagcatttt aaaggtattc atacagaata
     1441 tgaaaaccta aaatttactt caaaaatgta tctttggact gaaagaggag caaacagaca
     1501 ctgtaaaata ttagatactg ataaagcttg ggaacaattc gataatttag aagaaacata
     1561 tttcaaagtt aagcaacata aaccatcttg catagaagat gttttgatag aaagtttaaa
     1621 agaaatgaaa gatttaagac tacaagttaa ccgagctaat aacattgcat tagaagcgaa
     1681 ggcagaagtt gaaacaataa aagatgtagt ttcattagac tcaaatagtt ggagaacaga
     1741 tacacatcaa ttaattgcaa ggatagcaaa aaaacaaggt ggttttgaac atataaacac
     1801 gcttagaaca gaaagctacg aattactaaa taagcgattt ggagttgacc tacatagaag
     1861 gttaatcaat aaaagaagaa agatggcaga agaaggtgta tctgaatcca aaagagagaa
     1921 agttaacaat ttagatgtaa tacaagatga taagaaactc atagaggggt atgttgctat
     1981 tgtaaaagat atggctttaa agtacggaat atcaagtgat ttaagtaaga attaggttaa
     2041 ataactgtag cactttagaa attaaataca gaatattttg aaatatattg ttttaattaa
     2101 ttattaacta ggaggttgtt aaatacatga aaaataatac aagtgattca agagtaagat
     2161 atttttgcaa gtgtccatac tgtgggtttg ataatgaggt agaagttaaa aaaaggattg
     2221 aagcctaaga tatgttgcat gtgtacaaaa gaagttgagt atgaaaaact ggagcaacaa
     2281 agtgattcag aaaatataga aattaaagga gtgtgtaatt aaatgaaaat ttcatttgaa
     2341 agtgtagtga acaatgtaaa taatgaaaca gaattaatat tatcaaaaga agagttacaa
     2401 atagctaaaa ggatacttaa tatattgaat gaaaatgaac aaagtatcct ttcctcaaaa
     2461 gatattctag atttttgcaa agaagctctt aaatataatt tagtgcctac gtttgtttaa
     2521 agacactgag accatcacct ttagaaaaac tattattgac ttctgaaagt ttcaaaaaga
     2581 tttcatggta atgttttaaa agttcttctt cagaagaatt tttaaaatca tattttttct
     2641 taagaatttc taaagtaaga gtatgtaata tatctttact gaactgcata atatcaccaa
     2701 ctttcataaa aagatatagg atatatccta caaatatatt atatcaaagg aggaaattaa
     2761 tggcaattaa tgacaacata aataaaattt taagagatag aaacataaaa gcatggaaat
     2821 tagcaaaaga aataggcgta gattcaggaa atttatattc aattttaaga gggaaaaata
     2881 aaaatccaac gatagataca ttgataaaac tagctgatta tttagatgtt acattagacg
     2941 aattagttgg aagataaaaa ttaaatacag aatattttga aagtaggtgt tttagatgac
     3001 aaaagtagta gctaaagaac aattatttta tagagcaaaa gatgtagcta aattcttaga
     3061 tatatgcgaa gcaactgcat acaaaataat tgcggaatta aatgaagaat tagaaaaaga
     3121 aggttttaag actttttctg gcagggtatc aattgcttat ttcaaagaga gatattgcta
     3181 taagccaaga aagggggtga tttaattgaa tgtaaaggta ctaatagctt atatacagtt
     3241 ttgtaagcaa tataataaga aagcaagttt tgaaggtctt aaaaaataca acaaaggggt
     3301 aattgcatga gaataattta taaaaacaaa atctacaaag tagaacaaga caaagtgtta
     3361 tttagaatta catactatga tgagcagaaa aataacagga agtttaataa taataagaaa
     3421 gtaaaaagaa gcgttttaac aagagatata gaggtagtta acttgtattt accaacacat
     3481 ttaaaaataa agtaagctat aaataattaa agaaaaaggt gattagatgg aaatagaaca
     3541 aacaacaata cgcctgtcta gagaacttaa agacaagctt ctaaaacagg caaaagttaa
     3601 aggttatacg ctaaaagata tggtagtttt tattctaaag gattatcttc aaaatatttc
     3661 tcaagaataa attcaatttc tctacccaca gaacgcttat ctttttgagc aagttgttct
     3721 attttttcaa aaagaagttt attaattctt agtgtaaatc tcttatcttc ttcacgagta
     3781 taaatatctt tattagacat atttatcatt ccttaataca aaatttgacg tctttatgac
     3841 actttaataa taaaataatt aatgaagaaa gtcaataaaa atgcttgacg tcaaatgtat
     3901 gacatggtat tattaaaaca agaaaggggt tgacgtcaaa tgtatgacaa agacgagaga
     3961 gtaaggttta cttttagatt accagcaaca ttacttgaaa aaatgaaaga tatagcatca
     4021 aaagaaggaa gttctatgaa ctcgctaata ttgcacatac tttgggatta cataaaagaa
     4081 atagaaaaat aaggaggtaa ttatgaataa attcgattta aagaaagttg aagtaggaaa
     4141 tgcagtaaaa gtaaattgca aaagatttgg ttttgaaatt gattgcattg tagtagtagc
     4201 aacagaaaat gagctaaatt tagcttacta cgacaaagaa agaggatgta tggagtatca
     4261 agcattaata gtagaagatg ttagatatgg tgattatgag attttatttt taaattaggg
     4321 ggtgtatttg atggaattag ctatatcagt tgcaacaagt caaattaaac aagcaaagaa
     4381 aaacataaag gaattaaaag caaaagagga ttatgacaaa gacactctaa gctggtggga
     4441 aggtgttaaa caagctagcg agaatatact agagttttta gaaatagaaa ataaagcata
     4501 agaaaagagc ctagggtgag gctctaatca aaaaaacaaa aaataaattt aataatatgt
     4561 attataacat aagggggaac taaaatgaaa gcaattttat taaaaagttt aaatatagaa
     4621 aatttcaaag gagtaaaaga gcttcacata gattttaaca atgtaactaa tgtatttgga
     4681 gaaaatgcga ctggtaaaac aagtatattt gatgccttta cgtgggtaat gtttgataaa
     4741 gatagtaaga atagaagtgt atttgagata aagcctttag ataaacaaaa caaagtcatt
     4801 agaggacttg tgacgactgt tacagcagta ttagaagtta ataataaaga gataaagtta
     4861 actaagaaat acgaagaaaa atggactaga aaaagaggag aatcagaagc aacttttact
     4921 aaaaatgaaa caacttacat gataaatgat actccaataa aaaagagtga atatgtaaaa
     4981 gatatagcag aaatagcaga cgaggaccag tttaaattgc ttaccaatcc ttattatttt
     5041 tcaaatgaac ttaactggaa aaaagctagg gaagtgattt tagagatatg tggagatata
     5101 acaacagaac aaattataga agctaaacag gagctaactc cacttattac agaatttgaa
     5161 aaggaaaata acatagataa gattattaag aatagaaaag ctactaagaa taatctatct
     5221 aaagaaaaag aagaaatacc agttcgtata aatgagtgtg ataaaagcat gtacagtata
     5281 gattttgagg aaatagaaac tcaacttaat gctaaaaaag ttagcttaga agttatcgag
     5341 gatgagttac tgagtggtac aaaggcaaat gagcagattt taaaagataa agaaaaagta
     5401 tttgaactga aacaagagat acaaagtatt aaacaatcag cgagtgaaag aggaaataag
     5461 aagagaaatg agttaataaa tgaaaaagaa ggtttaaaat ataatgtcaa aacattaaga
     5521 aataaccttg tatatttaga agaaagcaga aaactaaaag aaaattcgag aaataaacta
     5581 gttgaagaaa caaataatct gagaaatgaa tggtctaaaa agagccaaga ggtattagat
     5641 ttaaatacca tacaaacaga gtgtccaact tgtaaaagac ctctagaaac tagcgatata
     5701 gaagttaaaa aacaagaaat actagataat tttaatctaa ataaagcaaa tgtattaaaa
     5761 gaaataggag tattaggaaa atctaaaggt gaagaagtaa aaaaacttaa tacagaaatt
     5821 gaacagctac aactagatga aaaagaagcc ttaaaagaga tacaagaaaa agcagtacta
     5881 atagataaga taaaaaaaga gttagaaaac acaaaatcta cagaggtata ctctttagat
     5941 gaagaaaata gactggttga aattgatgca gaaataaaag aattagaaga aaaaataaac
     6001 aataaagatg ataataaaaa cattgatgaa ctaaaagaaa acaagaaaaa actagctgtt
     6061 gaaattgagt tacttaataa agaactagct aaaagagata ttaacaaaga actattagat
     6121 agaaaagagc agttgttaga aaaagaaaaa gcgttaggta tagaattagc tcatcaagaa
     6181 aaaatactca acttatgtga gttatttata aaaactaaag ttagtttatt agaaagtaat
     6241 ataagtagta aattcaaaaa tgtaacattt aagttattca aagaacaaat aaatggaggc
     6301 attgaagaaa cttgtgaagc tttaataaat ggtgtgcctt tcagcaatgt caatacggca
     6361 ggacagataa atggagggct agatataata aacactctat ctaatcattt tggagttaaa
     6421 atgccaatat tcattgataa cagggaaagc gtgaatactt tgattgatat tgatagccaa
     6481 gtgataaatt tagttgtaag taatgataat ccattaaaaa tagaaggagt gaactaaaca
     6541 tggcaaatga aatacaaaga caagtaagtg ttaagaattt actttctaca gaagcttata
     6601 agaaaaggtt taaagaggtt ttaaaagaca aggctaatac atttatggca tcagttgtta
     6661 atgtatctaa cttaccaagt ttaaaagatg cagaacctaa ttcaatttta aaatccgcta
     6721 tggttgcagc aacattagac ttaccaatag acccgaattt aggcttttct tacttagtac
     6781 cctttacaaa taaaggagtt aaagaagcac aatttcaaat tggttataaa gggtttatac
     6841 aactagctat gaggactgga cagtataaaa caattaatgc aatagaaatc tatgagggtg
     6901 agattaaaag cgttaatagg ctgactggag agatagagtt taataaaaat gaagatgaaa
     6961 tagacaaaga gattgttgtt ggatatatag catattttaa attgttaaat ggatttgaaa
     7021 aaactctata catgagtaaa gaagatatgg agaaatacgc taaaagatat agccagacat
     7081 acaaaagtaa taaggattat gttgtaaaat caagtctttg gacaactgac tttgatgcta
     7141 tggcagttaa aacagtactt aaaagattgc tttctaaata cggaatatta agtatagaaa
     7201 tgcagaaagc tttagaaact gaccaagcag ttataaagga tgataattca gttgaatatg
     7261 tagacagaca agtagaagaa gaaatagaag aaaatgctaa taaaaagact atagatatga
     7321 caacagaaga aaaggaaaaa gctattgaaa ttaaagaatc taagcctgaa gaagtaaagc
     7381 aacaggaatt tgaagcacca ccgttttaat gaaaattaaa gtgttaggga gcagtagcag
     7441 aggtaattgt tacttgctcc aactaaaaaa agaaacatta atacttgagt gtggaattaa
     7501 ctataaagat atattaaaag gcttagattt caatttagaa agtgttattg ggtgtttggt
     7561 cacacatgaa cataaagacc attctaaggc tatcacagag cttacaaata atggaataga
     7621 tgtatattct agtaaaggta ctttagaagt tttaggaata gaaaatcata gaactaaagt
     7681 tatagaaagt gagaaattat ttaagatagg taattttaat ataatgcctt tctcaactaa
     7741 acatgatgca gtagagccac ttggattttt aataaatcat gaagaaattg gtaatctact
     7801 tttcatcaca gatagttact attgtgaata taactttaag aatttaaatc acatcatgat
     7861 tgaatgtaac tatagtaaag atttattaga caacaataaa aataagattt atttgagaaa
     7921 tagaatagtt aaaagtcact ttgagttaag taatgtaatt aattttttaa aagctaatga
     7981 tttaagtaat ataaaaacaa ttactttact acatctaagt gaagataaca gtgataagga
     8041 tttatttata aaagagatag aaaaaaatgt aggcatacca gtaatagtag ctgagaaagg
     8101 gctagaaatt tatttagact agaggtgata agatggcaaa atatagaatt ttacaagcta
     8161 acttttggga tgatgctttt gtgctagatt taactcctga agaaaaatat ttttacaatt
     8221 atttacttac aaatggtaga gcaagtcaat gtgggtgtta tgaactgcca tataaaatta
     8281 tggaaatgca aacaggatac aacagagaaa ctgtagagaa gttaataaaa agatttatag
     8341 agtatggaaa aataaaatat gattctgaga ctaaagagat attaataatt aattggagta
     8401 aacataattt ttctaaaagt ccaaaagtac gtacttgtat cttaaaagag gttgaaaata
     8461 ttaaaaataa agaatttaga gagtatatgt atagagtatg tatagactat ggatacccta
     8521 tcgatacagt atctatagac tatagggaaa aagaaaaaca acaacaaaaa gaaaaagaaa
     8581 aagaaaaaca acaacaaaaa gaaaaagaag aaaaatcttt ggtggttgtg gataacataa
     8641 aaagatattt tgatttagaa tctaaagaca ttgaaaagat tgttgatgta tttatacata
     8701 caggaagagg aattgactat ttagaggaaa aactaatgtt agtcaaaaat acggagaatg
     8761 taaaaagcgt tacaggatat ctcataaaag cattaaaaga agattataag cttataccaa
     8821 gcaagaacaa taaaaataaa tttagaaact ttgatgaaac atttactaag tacacatctg
     8881 atgaactaga cgacattatt aagaaaagtc aaaaagagaa gtttggagta gggagttaat
     8941 ataactatta acttcctaga agttaaaata ttggagggat gaaagatgga gttaaaattt
     9001 agagaatgga ataaaaatgg taaagaaatg tatagttatg atgaaatggt gtgctattct
     9061 aaaaatttgc ttagagagtg ggtttatagt ggagtttatt taccaacagg caatgaaaat
     9121 tctgaggtta tgatatatac aggtttgaaa gattgtatta aaaaagaaat ctatgagggt
     9181 gatattgttt catacatctt atcatttgaa gaatttatag gagaggtaaa atttgaagaa
     9241 ggtttctttg taatagataa tgaagtgcta ggagaatgcg ttgggttatt tcatgaaatt
     9301 gcagtggtta aagttattgg caatatatat gaaaatcctg agatgttgga aaagataagg
     9361 aaacctaaag tattggaggg atagaaatga gatttgagat aggtaagact tataagtttg
     9421 ataaagagaa atttatagaa attaatggtg tagaacacta taaaaaatat aaagaacttt
     9481 ggattgataa cattgaaggt gttgaattta ctgttaagaa aatatttgaa aatggctatg
     9541 ttcattatcc aaatgaattc tattttgctt tcggtatagt tccagaatgg tgtgttgaag
     9601 ttaaggaagt aaggggtatt agggatgata ataattagaa gtcaagatag attagattta
     9661 acgagagtta atagagttga aataagcaat aatcgagtat atgcaatgtt tgaagatggt
     9721 attagaaaaa taggtgaata tgaaagtaat gaaagagcta tacaagtgtt aaatgagata
     9781 caaaagttta ttgaaagtgg agtgaaaatg gattatatag attcttgcag agttagacat
     9841 aatcaagaga aagtatttga aatgccagtt aaataaaaag ggggaactaa atatggctaa
     9901 ggtttgggtg gatgcaggaa catttttaga aagaactaaa gatataggag atattgaatg
     9961 taatctaaga gaaatgataa ataaaaataa acaaaaaaat ttaattggta gaataaattc
    10021 caaagggcaa ggaagaaaaa gtaagaaagt agagtgtact aacatcatta ctggagagag
    10081 taaaatattt gtcagtgctg tcgaagcaag taaatattta tattttacag aacttcatat
    10141 tactcgtcta gcaagaacag gaaagactac taaaaatggt tggaaagtta gatatattca
    10201 agaggtgaca gatggtatta gcaaatgtgg agcaagtaat taaattagct gaaaagataa
    10261 taaataagaa aaagtgttct gttaataaag ctattgatat agctataaaa atattaagta
    10321 gatatgaata tgaggggatg attaaatgag tttgattgaa tacagaggct atgattttga
    10381 aaataaaaag tggatttatt ctaaaacgat aatgtggagt aatgctgtag actgtttatt
    10441 aatgctgaaa gaagatttta agtggcgaaa agtttgtaat gttggaatat gttctgaaga
    10501 atgggctaga aataatcaag aaatctatga aggggatata ttgaaagaat atgataattt
    10561 caatgacaca agccaatatg gagttgtaaa aagggatgtt ggtagtatta aaatatattt
    10621 ggaatggcat tatctaaaga agtttgaagg agaatggata gagattgtaa ataaaacaga
    10681 aatatatcat agcagagatt acaaagtggt aggaaatgaa tacgagaatt tagaggaagt
    10741 tagaaaagag ctcttagaac gtaaggagaa acttgagaat gaaaatacaa atgcagttaa
    10801 ctaaagaaaa ggagtttttc aaagtttatg taaattcaga agaagaagag ttggagaaat
    10861 tgttttatga atttgtatca caaatgttag cttgtaaaag aaaaaataag aaagttcaag
    10921 gagatattga gaaatgaaat taaaagatat tataaaactt ggagaaaagt attgctattg
    10981 tcctagttgt ggtaatgaca agataggaaa caatgaaggt aaattaatag ttgaagaaca
    11041 cacatattat agagaatgta aatgtgggtt taatatatta attgatgata ggaaggatga
    11101 aatctaatga atatcttaac tagtttactt ttgataggga ttagttttgt agcaggtagg
    11161 gtttatgagt atagattgaa tctgaaagag tgtgaaaatt gcgacaataa aagaggtgta
    11221 taagaatgga tgatagatta gagatttgca aaaaaatgtt tcctaacgtt actaaaaata
    11281 ctagattact ttgtcattat tgcgatggta gaaatatttg cagttatgat gaagaaaaag
    11341 ctcgtaaatt attgagagaa aaagaggagg ttaagaatga ctaattttga aatgataaaa
    11401 aatatgagta aaaaagagtt ggctgaattt ataaacgaaa ttggtgataa ttgtacttgt
    11461 atttactgca gttgtcaata tcgatatgaa atttgtgatg tagatgaaaa atgtgttgat
    11521 ggatgcaaag agtggcttga catggaggta gagctataaa aatttggaat gatgaaataa
    11581 gcaaagaaat agaaagatta aaaggatttt tatataggga aaatatctaa ttaaaacagt
    11641 ttagagagtt gcaaaatgta ttttaatata aattgttgtt gaagtgtttt gtaactctca
    11701 aaaatgaaaa taaggggtga acaaatgtca aagtatgtac ttagatggca aaaagggtta
    11761 ttgttggatg aacgtaagat aaattattca tgtggaagta aagaaatgtt aaaacaaaaa
    11821 gcagaattat tagctaaaga tgacaagata ttacttgtaa caatagataa agttgaagaa
    11881 gttataaaag atactagaag tcagaaaatg gctgaatatt ttgaggatga aggggtggaa
    11941 ttatgataat acacaaattt ataatacatg ttttggataa gaatagcgat acaccaatac
    12001 taaatgattt tgaaggtaga gttagtcaag atatggacat attctttcaa aagaaaatca
    12061 acaaagtatt aagagataat gacatcagaa aagcagtatt taatgactat agtagcaatc
    12121 tgattaagaa atgttgtgaa caaattattt atgatgaaag ttcattttta aataactcta
    12181 aagagattgc agcttattta tttgatgtta tgaaattgaa tgctacatta gaatcttgcg
    12241 acttagcaat atgtttatat actgaaaaag atgaaaagaa agttgctata ttaaaacttg
    12301 attacaacaa gtcgtatact cattctatta gctttgaaga tgataaattt aatatacaaa
    12361 tgtctaagaa tgaaattaat atacaagaga ctaaagcaat taaaataggg gcaattattg
    12421 gtttgagtgg aatgaatgac gaatatcatc taaaagtatt ggataaggat gcagagaagg
    12481 aagaagctaa ttctaagttt gttacagggt ttctaaatgc tactaagata aaagatgata
    12541 aatttaagac taagatgttt aaaggcttgg tagatgcttt tatagcgaat ttatatagtg
    12601 acatgaagca gggcgaagat gtaagaaata tactgcttta catgctaaga gaaaagcaaa
    12661 aacttgatat aaatgagttt actgacaagg caataaaggg agatttaaaa gatagtttta
    12721 aggatcatgt agaagaaaaa ggtcttgatg gaagttttaa tattgataaa aagtgggttg
    12781 agaaaaatct aaaaaataga catataaaaa cagatacagg gattgagata aaaggcaaaa
    12841 tagatgattt tgaggatttt atgaaatatg gcattagaca taatgggaat ggaactgtag
    12901 atataattat taaaaatgta agtttttata atgaaaaata aggaggttac aatgaaagaa
    12961 caaggtcgtt tggtactaaa taaacttgga acagtagatg aagtaatgaa aaaagttgaa
    13021 acggatgaaa aagaaataaa tgtttattta aaagaactaa aagaatgtga aaaagcaaaa
    13081 gttttgatta ttggtaaaga tggaactgaa attccaatag ttttcaaaga tgaaactatg
    13141 aacgaatggt taagaaaaga aattgagata agttttgatg aatttaaaga tgaaattgtg
    13201 caggtcaaac aagtattact taaaattaca ggagataatt agaaagctgg aaggtgctca
    13261 tatgactaac aaagaaatgt gcaagtcaaa gaatatcaca gagaaagaaa gttataagcg
    13321 atttagtaaa gaaatttgta aaagctgtaa aaataataag agagattgtg aaagtaaaga
    13381 ttgtgatata acatacaaaa attggttgga ggatatttac tatgtatagt acttataaag
    13441 taaaacttaa aactaaaaga acattggagc aagtaagaaa tcaaagtgta gattttgagt
    13501 attcagaaga aggattaaaa aatacgctaa aatactataa cttgattgat agtctagaag
    13561 taattgtagt taaatttgga gatgaatatt gtctagttga ttgcgatgaa gaagatgaaa
    13621 aaacaataat ggaagcatat tatcttttag aacaagacga atatactgga tgttatataa
    13681 atgaatatga gttatttaaa aaagattggg aaaatggtag ctattacggg gaagtctgta
    13741 tggtgttttc ggatgatgaa attgagataa ttgaaaagtt aagggagggt taaatatgaa
    13801 taaaagaatc aaattaaaaa aaagaatact tcataaaata tgtcaagaaa actataagga
    13861 attaagaaaa taaaataaat agtcaaggta agtttgtgaa tgaaactaga atgttataga
    13921 cttactttga cttataaaag tgagtgtgtt aaatggctaa tatatattgt gaaaattata
    13981 attgcgaaaa ttactttgaa gatatgtgta tgcttgaaag aattgaaatt aataacctga
    14041 aagtgtgtga aagttacatt gaaggtaaaa atgagctata taaattagaa aacggatata
    14101 ctatacatcc taaagatttg aaaatggtga aaagtaaaga ttattctgtt gaagttactc
    14161 atattccaac tggtattaca gtaaaatgtc gttctacaaa tagtatttta aaaaataaaa
    14221 acaagtgttt ggaagctcta gaagaagaac taataaaaat aaactcacac ttagagctag
    14281 aagatttatt ctaaatagga agtgagctta tgaaacgaag aatatgcagt tggtgtggaa
    14341 aattatttta tcttaaggaa aaatctaagg atatttattg ttgtaaggac tgtagaaaga
    14401 aagctaagaa ggtgaaaaat gaaagttaat tttacaatag atggagaacc aatcggaaaa
    14461 gaaagaccta gatttaattt gtctactaaa agaacctata cccctaataa gactagagat
    14521 tatgaggacc taataaaatg gctatatcaa tctaaagtta aacattactt tgaaggttat
    14581 ataaaaatga ctttaagatg ttactattct atagctaaaa gtaacagtaa aaaggttaaa
    14641 gagcagaaaa aaaataatgt gttaagaccc agtaagaaac ctgatattga caatgttgtt
    14701 aagattgttg ctgatgcact caatgagata gcttataagg atgatacaca gatagttgag
    14761 gttgtagcta gtaagtatta tagtgataga ccaaggattg aagttatatt ggaagatgtt
    14821 atttaaccaa cggaaaaatt cgttcgttag attaggtgct cagtttttca taaaaatgtg
    14881 aaaaagctaa ataaagaata tatcaaatga taaaggagag atagactatg aatgaaaaca
    14941 taaataaaga aataacagta cttggaactt tagaagttga gggaatgaaa tttcatgata
    15001 ttgagggtgg gtttggaaaa ggcaagaaag caatgctagt aaaggatata gccgagatac
    15061 ataatagaga acttaaacat ataaatgagc taattaataa taatataaag agatttaaaa
    15121 caaatataga tattatagat ttaaaagtga gtcgttctga acgactaggt gatttaaatg
    15181 ctaatccttt tgaaggatta ggttacgagg ctgttgggta tagtaaacaa tcttttaatc
    15241 agtcaagaaa tgtttacttg ttatctgaaa gaggttattc aaaactactt aaaatattgg
    15301 aggatgatgt agcttgggaa caatatgaaa aaatagttga tggatatttc tctatgagaa
    15361 aagaattaaa taatcctctt ttaagtgcat caaaagagtt acaggctata tttatgctag
    15421 ataagaaaca agaagtctta gagactaaaa tagagaatgt taatgagaaa ttagagaatt
    15481 ttatagatga tgctccatta tttaatatag agtgtgagtg tattgttaaa gaagttaaga
    15541 gagtagcaac aaaatcactt ggtggacatg gaagtaaggc ttataaaaat aaatctttaa
    15601 gaggtaaagt atacagtgat atatatcatc agattaaacg agaatttgga gtagatagtt
    15661 ataaggctat aaagcgttgt caattaaata aagtattaga gattgtaaac agttataagt
    15721 tacctatagt gtttgaggaa gaaataagac ttttgaatag tcaattatca atagtaagtt
    15781 aagaaaaaag aagaaggagt gctcccacac tccacttgtc aaaaatataa aacttttata
    15841 tacaaatatt attataacat aaataattga taggagtgtg tgagtatgtc taaaactaaa
    15901 aaagagtttt ttaatgcaac taagaaacaa ctttctaatt acaaacaatt aaatgcaaat
    15961 ataataaaat taaaaaatga aatacaaatg ttgaaagata attcagttgg agatttaaca
    16021 aaagctataa gttatgatag tgtcaaaaca ggaaaaacaa acaaaactag caatatgatt
    16081 gaggatgcta ttgttaatgt atcagactta ataacagaaa aagaaataga gttatatgaa
    16141 gcagaaataa ttaaatctac aatagattta gctataagga atttaaaacc tatacacaga
    16201 caaattattg aacttaaata tatagatggt ctaatgtggc aagaaatggt tgatataata
    16261 catttagaag aaagacaatt aagtgtaaga gctagtcaag ctattagctc aatatcaata
    16321 gcactgtttg ggaagaaagc attaatagag caagaaccac tttttgaatt gttagattac
    16381 aaactaaatt aaaaagtaag agtaattttg agtgctgaaa atgtgcaggt ttttttgttt
    16441 tagacatgag ataatagtat tgtggaaata aagatttccc tctcaaaact taataattgg
    16501 ctagggtata agggattgcc ctagccacta ttcaataata gagaaagtaa taatatattt
    16561 gcgaatatgt tatagataag aaaggatgcc aaaattgcac gctctattat tataaagggc
    16621 ttagatgtca gcgacagaaa gtcttttatg tgaacagact aggcagagcg tgaggacgct
    16681 gttagttcaa ttctaactat gttcaaatat taatctaacg gatacactaa aaagtagaaa
    16741 aattgagggc aaaattttat attttgtatc ttaattcaga agtctaaaaa atcgggtggg
    16801 gcttggtaac ctcactcacc atgcaagtac tggtttagtc taggttcgat tcctagaact
    16861 tgcgacataa tatgtatctc cctattaaaa agactaagtt tgggtaagct tagtctttta
    16921 atttttaaaa agaaaaaaga aatttttatt gtcataatac tatttgttta ggtatattat
    16981 aatgtgcata cttaaaatta aatacttagc aagtatttga attaatatac ataaaatata
    17041 tgacataatt tttttatagt gtaagttatt taaattaata caaataacag taattttatt
    17101 atataaaatg tacatattgt gaataataat aataaaatca tgtacaaaat gccaactgat
    17161 aattcctcga aatgtattgc acattt
//
LOCUS       DAGQWR010000087        17075 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.087, whole genome shotgun sequence.
ACCESSION   DAGQWR010000087 DAGQWR010000000
VERSION     DAGQWR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17075
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.087"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            69..938
                     /locus_tag="MIW95_002531"
                     /pseudo
     CDS             69..938
                     /locus_tag="MIW95_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
     gene            1844..2551
                     /locus_tag="MIW95_002532"
     CDS             1844..2551
                     /locus_tag="MIW95_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBY3151849.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            3163..3663
                     /locus_tag="MIW95_002533"
     CDS             3163..3663
                     /locus_tag="MIW95_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3151850.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(3913..4692)
                     /locus_tag="MIW95_002534"
     CDS             complement(3913..4692)
                     /locus_tag="MIW95_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151851.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKALDKENNVLGKSKALSFEYKNGNDEISLDTANNVLEKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(4689..5168)
                     /locus_tag="MIW95_002535"
     CDS             complement(4689..5168)
                     /locus_tag="MIW95_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY3151852.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRTLLKTKLENTEVII"
     gene            5974..6675
                     /locus_tag="MIW95_002536"
     CDS             5974..6675
                     /locus_tag="MIW95_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBY3151853.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKSALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLLVVFILGHRMRGIVEKMNVKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAVISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIVLS"
     gene            6701..7357
                     /locus_tag="MIW95_002537"
     CDS             6701..7357
                     /locus_tag="MIW95_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151854.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGLRFGQMPMLSVIFQI
                     WGLVATTYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFITDSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDSNKTKE
                     "
     gene            7564..9204
                     /locus_tag="MIW95_002538"
     CDS             7564..9204
                     /locus_tag="MIW95_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBY3151855.1"
                     /translation="MFDVKIANGIIIDGTGNSRYKGDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPIKLNLHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDKVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWDGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            9563..10885
                     /locus_tag="MIW95_002539"
     CDS             9563..10885
                     /locus_tag="MIW95_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151856.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMLKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDICCC
                     ITGLEEAYHAMKKENIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPAVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAI
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            11082..12089
                     /locus_tag="MIW95_002540"
     CDS             11082..12089
                     /locus_tag="MIW95_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBY3151857.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGTMGCPPEILKKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            12127..13524
                     /locus_tag="MIW95_002541"
     CDS             12127..13524
                     /locus_tag="MIW95_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBY3151858.1"
                     /translation="MASTNKNNVTSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKINTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIFKGEDN
                     MSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKITHNIWE
                     EKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIWSNFGA
                     DGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDNLWIVT
                     QLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEMMLEMT
                     SRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYEVERVT
                     CNQREKNEGYDVSIRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKEEWITF
                     DGMDVLKQMYMGVEE"
     gene            13669..14934
                     /locus_tag="MIW95_002542"
     CDS             13669..14934
                     /locus_tag="MIW95_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3151859.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTDIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            14976..16015
                     /locus_tag="MIW95_002543"
                     /pseudo
     CDS             14976..16015
                     /locus_tag="MIW95_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
     gene            16050..17072
                     /locus_tag="MIW95_002544"
     CDS             16050..17072
                     /locus_tag="MIW95_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBY3151860.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMVAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
BASE COUNT         6272 a         1875 c         3121 g         5807 t
ORIGIN      
        1 aaacgtataa agtttattat cggaaatttc tatattaata tgtagtaatt caatattatt
       61 ataaaggagt ggttattatt aaggcaattg aactttataa aaaattagat ttagaattta
      121 atattaaaaa tattaatgat gattggagtt ttatgaattt tgaaaatgat tttatcactc
      181 cagaatttag aaaaaagtac atatgattag tattagataa tgctcaaaat attaataaag
      241 tatacacaac tacttttcct gataaagaaa taattaagca agttatagat aaagatgaaa
      301 aagatatttt aattttttca catcacgcaa tgggatacat agcatcagat gaaggttttc
      361 catttcacga cataccacta tcttatatgg aagagatgaa aaatcgtaga atttcttttt
      421 atgtattaca ttctccacta gacaactata gtgactattc aacttctgtt agttttgcaa
      481 aagctatggg tttagaaata gtcaaaccat tttgtaaata tgacgataaa attgaggtag
      541 gagttatatg taaaacatca ctaaaatcaa tagaagagat taaggaatta attaaaaaat
      601 ctgttggtca tgatgtgaaa ttgtatgatt atggagattc tgtattaaaa gatggtttag
      661 ttgctattgc agcaggggga ggaagttatc catttgtagc aagagaagtt gctgaacttg
      721 gtataaatct atatattact gggtttacta agcctttaaa acattttgaa ccagtattag
      781 agttccatca aatagcaaaa gataatttaa ttaatgttat aggtgctact cattattcta
      841 cagaaaaatt tgcgtgtatg ggcatggttg attattttaa aaatctaggt ttagaatcag
      901 agtttttgca aggaaaatat tacttagagg atttatagga catattaaac aataaaaata
      961 aacagttagt aaaaatttat tttactaact gtttattttt ttacctattt atccagtgtt
     1021 ttgaaaaaat ctatctttat tttttaaact aagaaaatag acttttaatt gttgattaga
     1081 agatttagtt ttgatgtctt ttaaattatt tttcgatatt ttttagttta ttttttgatg
     1141 tattttagat tattatttag gattgattag aatttataaa tatttatttt agattattgt
     1201 aaatatttat ttaaaaaaaa tagaattatt atatttatga taataaatgg tataataaaa
     1261 ggtaaagtgt caaatatgca ctaaattaaa taagttaatt agtatatagg ttaaagtgga
     1321 agtaattaaa acttattaag tattagaaaa tgacttctta ttagagtaag tatctaaaat
     1381 gatataaaaa ttatgattac tcattaaagt gactattata gagtagataa aattgcccat
     1441 aaaaatagct ataatagagt ggataagatt actcattaaa gcaattacta tagagtagat
     1501 aaaattactc actaaaataa ctataataga gtggataaga ttattcatta aagcgattac
     1561 tgtggagtag ataaaattac tcactaaaat aactataata gagtagataa gatactcatt
     1621 aaggtgatta ttgtagagta gataaaatta cccatcaaag ttaattatca catattagat
     1681 aagattattc attaaagtta ctatcacaga gtggataaaa ttacacatta aaattgtctg
     1741 ttaaagaatg aataaaaaag agattgctca atataagaag catataaaaa atcaaacata
     1801 taaaatttaa taaataataa ctgataggga aaggaaagat agaatgatat ttacaaaaag
     1861 caataaatac gataaggact ttctcatgaa aaatatgatg ggacctaatt gtataaaaat
     1921 attggaagaa ttaacaagta agataaaact agaaaaggga atgcgtattt tagacttagg
     1981 ttgtggtaag ggtatatctt cgatattttt agcaaaggaa tttgatgcta ctgtatttgc
     2041 aacagattta tggatagaac caactgaaaa ctataaaaga tttaaagagt ttaaattgga
     2101 tgataaaata tttccaatac aggcagaggc acatgaacta ccttatgcag aaggtttctt
     2161 tgatgcggtg ataagtatcg attcttatca ctattttgga aacaaagaag gatttttaga
     2221 caatcatata tcacctcttg taaaagaggg gggaatactt gctatggcaa tgcctggatt
     2281 aaaagaagat tttgtggatt gtataccaga tgaactcatt ccattttggc aagataatat
     2341 gaacttccat tcaattacat ggtggaataa actttggtct gaatcggagt ctgtgattgt
     2401 agagaaatgt gaagctttaa attgtcatga tgaagcttgg aaggattgga taaattgtga
     2461 taattcatat gctataaatg acaaaaaaat gatggaagta gagaatggaa agtattttaa
     2521 tacaatttct ttgatagcga gaactaaata attgtgatga atttataaaa gtataagata
     2581 agtagaaata taagttgaat gagagtataa gaagcataga aatataagtt gaattaaagt
     2641 agaaggtaaa taaaaatgta agttaaataa agagtttata caaaaatgat tttttagatt
     2701 atttataccc aatcgagttg cttaggtcag ttgggtattt ttattttttt aaagttaaat
     2761 tgcaaatact attgtgtgat atatatttta atactaaatg tttgttttat aagaaataaa
     2821 ctaatagaaa aaatagtttt aacaatgaaa gcaaaaaagg tattgatgaa gattaaaaat
     2881 tttatagtac cattttatat atttttttat atattttatt atactttaaa tcaaatttca
     2941 aaatgattaa gtatatatac ccaaaagatt aaagaactat atttattata ctattaaaaa
     3001 ttattttaat agagatacaa gagatagata tattggtttt aattttatag aaatatacaa
     3061 agatatttta agctaatata tagtaattta tatagcatta taaactagaa tcatataaaa
     3121 taagttaact acaatcataa attaaaaagg agaaatgtta agttgataga aggaataaaa
     3181 tttataaagg cagaagaaaa gtatatttat tcgtattggc aaacttttga taaaatagca
     3241 aaagagagga aatatctagc tatggatgaa gcctttcctt ttgaagaaac agttgaattt
     3301 ataaaaaata ctataaataa aaacttacca caactgttta taattgattt agaatcagat
     3361 aactgtattg gttggtgtga cgttttacct aaaactgaaa aagttggtta tttaggcatg
     3421 ggtattctta aagagtatag ggaaaaaggt atcggaagta gtcttttaaa acaaattatt
     3481 gatttatcaa aagaatatgg gtatgaaaaa attgagcttg atgtatttaa aagtaatagt
     3541 agagctattc atgtttataa atctttaggg tttgtagagg taaatacaat atctagtggt
     3601 tttacatgga atgatagacc tgtaaaagaa gaagtaattc aaatggagct tacacttata
     3661 taaaataaag ttaaagtctt taaaattcca tatacacttg tataaaataa agttaaagcc
     3721 cttaaaattc catataaaag aaatattttt aagggctttt taataatggt tcttttgaag
     3781 gtagctaaat atctaaaatt ttatatatta aatttaaaga aaatataaag atggaagata
     3841 caaccatgtt tactaataaa aaaattaaag tcataaataa taaactcata aacaacttat
     3901 tattacttat gtttattttg catctattac ttcatgagcc attttaacta agtcattcat
     3961 agataaattt gagtcttgaa ctgtaaaaga atagtaaact ccatcttgtt tccatgtcaa
     4021 aaacttaaat tcattaactt caactttgtc tgattcatta tttggcccta cactaaatgt
     4081 gtatttgcct gatttttcat caattttatc ctgttctgtc attttataac cttctggtac
     4141 aaacttttgt gcgaatgctt cataagaaat attaatatct ttgtaagtat ctacaacttt
     4201 tagattgtca gaactcttct caagtacatt atttgctgtg tcaagagata tttcatcatt
     4261 gccattttta tattcaaaac taagtgcttt tgattttcct aaaacattat tttctttgtc
     4321 aagtgctttt ttatttacag tatatgctcc tttaaattta tatccattac taaattcaga
     4381 aactatatta ggggtaaagt taaagtcttt ttttacttgt tctgtagttg ggatagatgt
     4441 ataggtagcc ttagtacttg attttccaac taaactatat atatgtccta ctgcaagggc
     4501 tgttgtgctc aatatcaaag taatggctaa agttgcaact aagccttttc taaatggtaa
     4561 tttgtgtgtt ttattattaa tcattttata attttcctct ctttctggta aactatttag
     4621 tgttttactt aaactattat gaaaactttc tggaacttct ggaaacgaat tatttaatat
     4681 atttttattc atatgattac ctccgtgttt tctaatttag tttttaataa tgttcttcct
     4741 cttgataatc tactttttac agttccttga ggtatatcta atatttcctt aacctcatct
     4801 actgaataac cttcaatata gtaaaggact attggaattt taattttttt aggtagactt
     4861 tgaatagcag tatacaaatc gctatagtca tctttttcat caattttttt gtttttgaag
     4921 acatcttcaa atgatagaga ttttaatcgt ttgcgtctta atgaattaca ttcatttatg
     4981 actattctaa tcagccatgt tttaaagtat tgttcttcct taagagaatc caacttttca
     5041 tatgctttta aaatagcatt gttgacggca tcttcacagt cttggtcgtt tcctagaata
     5101 gacttagata ctcgatacaa agtctgttct gattcaagta tattgtttgt aaatgtagtt
     5161 ttatccaact gagttccttc ccttctatgc aaatcgtagc gctacttttt tgcctttcac
     5221 ttattagata aaattttact atagatggtt cacttttttt atgattattt gataaattta
     5281 agtttgaaag ttttgcctat tctatacaaa tattctataa tatatttttt tctagtattt
     5341 tcccctcata tataaagcca tgtagatata ttaaacttta gaagccattt atatatttag
     5401 ttttatagta agtgtttata ttaagtaatt ataaaatagt aagtatttga tgttgagtag
     5461 ttagaaaata atgcttttgg attatttact tcatgtatag atttaaaata agttttttaa
     5521 ttaatgacta aagataacgg tattttgact aaaagtaggc ttttaagaaa aaacaatgaa
     5581 acttaaatga gaatcaagag ttagaaatgt cattatttta gatagatatg aacataaata
     5641 aacttaaaaa atataaatta taaaaataat aattttttta aaataacaaa gttttttgat
     5701 taaaagtttt atattgaaat gtcaaagtaa taaaatacct tataagttaa ttacaggtta
     5761 aaaaaataat atgtgaattt tctgatattt actaattatc aataaagtat ttaagtatta
     5821 atgtttttag tgagacttta agacagataa tctcctatta aagatactat atcttagata
     5881 aaataaatta aaacaatata aaaacataac aagattacag aaagaaagct cataataaaa
     5941 cactaaaaaa tttattttaa ggaggaataa agtatgaagt ttcaagaaat gattaatagc
     6001 ccaggtcttt ggattgtttc tagtttttta gtaattattt cagttgttca agcaatcgta
     6061 tttatgaaat cagcacttaa agaggcttca aatcttggta ttgaaagaaa aggaattaaa
     6121 gcagcaattc gctcagctag tgttacagca attggaccct ctctatctcc tgttattaca
     6181 ttattatcac tggtagctgt gattggtgct cctacgactt ggatgaggct ttgtgatgta
     6241 ggagctgcca gaacagaact tggagtaata tctcttacat caaatttatc tggagtagaa
     6301 gtaggtagtg ctgcatttgg agcagaagct ttttcgtatg cactatgggg aatggcttta
     6361 aataacttag gttggttgct tgttgtattt attttgggtc atagaatgcg tggaattgtt
     6421 gaaaaaatga atgtgaaata taatcctact tgggttaaaa aactacttgc aggggctaca
     6481 gttggattat ttgcttacct tctttcaaat caaataaaaa cattcgaaat accaaaactt
     6541 acaccagcag ttatttcagc tatagttatg ctagtattga cgactttatt taaaaagaat
     6601 caacgattac aagaattatc cttaggtatt gccatgttga ttggtatgtt tggcacacag
     6661 attgtactat catgattaaa acagataaga ggtgataatt atgtatgata aaaagatgat
     6721 aaaaataggg agaatttcta taggtcttgc aattattgca aattttattc ctgcaatcta
     6781 tgtaggtctt cgctttggtc agatgccaat gctttctgtt atattccaaa tttggggatt
     6841 agttgctaca acttatggag tgagttggat tgttcaacct atagcttatt atccaacact
     6901 aggagctcca ggttcttata taggttggct tgcaggttct gttggagata tacgtatgcc
     6961 tgcagcatca atggcacaga aaattgcagg tgtagaagca ggaactcatg agggtgaagt
     7021 tgtaggaaca attggtacat gttgttcaat ctttgtatca gcttctatga ttactatttt
     7081 taccattgta ggctataaag taattccttt gttacctgaa tttattactg attcatttaa
     7141 ttatatactt ccagcattgt ttggggcaat ttacgttgat attgcgcgta aagatattcg
     7201 agctggtgga tgtacgattg ctgtagcatt agctattatg tactttggtg aaaaaattgg
     7261 tataccaggt ggattgttga cattgtttat tgtgttaggt gggatactta ttacaagggt
     7321 tttctttgta attgatagca ataaaacgaa agaataggat tgtttactag gaagaaagac
     7381 tagaattatt tgctgagaaa aagaatagga ttgtttacta ggaggaaaga ctagaattat
     7441 ttgctgagaa aaagaatagg attgtttact aggaggaaag actagaatta tttgttgaga
     7501 aaaaagaata ggattgttta ttaagaaata tgaatataaa taagtcttga aaggagaaga
     7561 agaatgtttg atgttaaaat tgccaatgga ataatcattg atggtacagg aaattcaaga
     7621 tataaaggtg atgtagggat tgtaggagac aaaatcatgg ctattggaga tctaagtcaa
     7681 aaagaggcta aagagactat agacgcaact ggaaaatttg tatcacctgg ttttatagat
     7741 ttccacactc attctgattt atctcttgtg tatgacaaat acacaagaag ccgtatccat
     7801 acaggtgtaa caacagacgt tattgcaaac tgtggtatag gagttgctcc gataagagaa
     7861 gagaagaagc aggagttaat tgactatctt ggaacgagga taattggtac aattccaata
     7921 aaattgaacc ttcactggaa tacaatgcag gaatactttg attacttaac tgaaaattct
     7981 ccagcagtaa atgttgtagc ttatgtagca caaggacctg tacgtatcaa tgaaatggga
     8041 ttttcaaagg agccagctac tttagagcaa cttaaaaata tgaagttgga agttagaaag
     8101 gctatggaag ctggatgtgt agctctttcg tcaggattag tttatttacc aggagcatat
     8161 acaaagaaag aagaaatggc tgaattatgt aaggaattga taccatacaa tggatactat
     8221 atatctcata ttagagatga gggtgatgaa gaaatggaag ctcttgatga agcaatttat
     8281 atagctaaga cggcaggagt tcctctacat atttctcatt taaaagtcat gggtcataag
     8341 aattttggaa ctattgataa ggtatttaaa aagctagatg aagctgaggc agatggcttg
     8401 gaagttactt ttgattgtta cccatataca gctggaatga catctttggg tgcactttta
     8461 cctccttggg cttttgaagg tggtgtagaa aacatggtaa aacgattaga agtgcaagaa
     8521 aatagggaca gaattataaa agagctagag gaagggattc ctggatggca atgtttctac
     8581 cagttagcag gtggttggga cggtgttgta ctagcctctg taatgactga agcaaataaa
     8641 tatgtagagg gtaaaacttt aatggaggta gctaaaatca atggtgaaaa tccatttgat
     8701 acgttcttta ggcttttgat tgaagaaaaa agtaaaattc aagtagttgt tcatacacaa
     8761 ggtcaagagg atacagataa ggtcgtttgc catccaaagt catgtatagg ttctgatagt
     8821 atggaccttt ctacagaagg cttactttct cttggaaagc ctcatccccg tgcatttggt
     8881 acttttggac gaattttctc ttactatgtt agagaaaaag gtatgttgac ttttgaggaa
     8941 gcagttaaaa agataactta tctttcagca aaacgcttag gaatttataa agaaaggggt
     9001 ctacttaagg aaaattattt tgctgacatt gtggtatttg acccagatac aatagaagat
     9061 aaagcgactt atagtaatcc taaacagtat acagtgggag tggaatatgt attggtaaat
     9121 ggtaaaattg cattggcagg aggaaagcaa actgatgtat gtgctggacg tgtaattaaa
     9181 aatcctctta gcatagcaaa atagaataca ttaagaactt ttataaaaat agtctttctt
     9241 tgaatattaa ggaaggctat ttttgttaaa ttaataaaga ttactctact tgtgttttct
     9301 agagaattta actttaagta gaaaaaacaa tatattaatt gtaaaatgta attaatatta
     9361 atggataaga tttaaataca aaaacttaaa tacaaaaatg ttttaacaga tagtcataat
     9421 taaaaaactt aaagtaactt tttatataag atataattga ttatgcaaaa ttttatgaag
     9481 cagcaacctt gttactttaa gtcaagggag gttcagaaag tgtcttaaga gataatagta
     9541 tctttaggaa aggagaaacg aaatgaaaat actttatgta ggaccaggca tggtaatcga
     9601 caaaatacta gcagtaagta aaaagaattt tcccaatatt gaagcagatt ttcttagata
     9661 tgatagatat attgaagtac ctgatttatt ggaaaaatat agaggtagag tagatgcagt
     9721 tctttttaca ggtaaatcgc catttaaatt ttttgaaaaa agacaaaaag attttgtgtt
     9781 gtctgattat attcctagac atgaaacaac attatacaga gtattgcttg aaattacata
     9841 tttgcttaaa ttcgatatta gtagacttag tatagatact tatgataaag ctatgctgaa
     9901 gagattgtat aaagaaattg gagttgaatt taaggatgaa catatgtttt ttgcagagca
     9961 aaagtattta gaagaggact acataaagtt tgtgcttgaa tttcataaat ataattatca
    10021 aaataatgac atatgttgtt gtattacagg tcttgaagaa gcatatcatg caatgaaaaa
    10081 agagaatatt ccagtagttt tagccttgcc ttctgaggat gtaatggtac aaagtataaa
    10141 aaacattcag atgcgttata ttgcaaagaa aaacaatgag aatcagattg ttgttcttgc
    10201 tatcaaactt aatatgcctt cagaatattc actgatgaag gaagatgagt acgcttatct
    10261 ttcacaaagg attaagattc ttgataaact ataccatttc aatagccgta tagatggtgt
    10321 tttggtagag cagagtagaa gcgaatttat gatttttaca actaaaaaaa tacttgagtt
    10381 agaaacaaag aagtacagag atatttattt gttggatatg attcgtgaag tttcagttat
    10441 aaacacttat ataggaattg gttatggaaa aacagcaaat gaagctaaat ttaacgctta
    10501 tgagagtatt aagatggctc aaagtcaaaa aaatgatgca gcatatgtag tttttgaaaa
    10561 tggagaaatt atgggaccac taaagccaaa tcctgctgtc aaagagaaaa atagctttga
    10621 tgaacgattt tatcaaattg ctacagagac agggctaagt gctaatactg tatataaagt
    10681 gttcaataaa ataataaata gtggaaaaac tgaatttaca tcaaaggaat tagctattat
    10741 atgtggtgta agtattagaa ctatggacag gattatactt agattgtgtg attctggtta
    10801 ttgtgaggta gtgagtgaac agcttatgag caagtatggt agacccagta gaatattacg
    10861 ttttaaacct tttctgttgt tttaaataga tataagttaa aaattaaatt tttatgataa
    10921 cattttcttt acaatttttt agatatatgt taatatttag aaaaatgcat atgtattcat
    10981 aaaataaact caaaatagct aaaaaatcat taaaaaggaa atcgtatgca taaaatgaga
    11041 aagttaattt ataacttaaa taaatctggg aggagataat tatgggaaaa tatcaggatt
    11101 caaaaaaagt tgtaagaaat tattttgaag cattagaaaa tgctactggg gatgaggttg
    11161 aaaatgtctt aaagcaacat atgaaatcag attataactg gaaaggtgtg tatccatttc
    11221 gtgaacaaga aggcactgaa aatgtagcag atgtattttg gaagccttta aaaaaatcat
    11281 tgagtaatat gcaaagacga caagatattt tcattgcagg aacaaatgag attgacaata
    11341 gtgaatgggt aatgagtatg gggcatttta tgggactttt tgataaggat tggcttggca
    11401 ttaaacatac tcgtaaaatg attagtttaa gatatgcaga atttaactgt gttcaagatg
    11461 gtaaaattgc taaaactgga ttatttgtag atataattgg gtttatgatt caagcaggag
    11521 ttaatccatt gccaccacaa acaggttcat attttgtata tccagggcca attgaccata
    11581 atggattact ttttgaagat gcagatgaag ttgaaggagt aaaaacttta gcattagtaa
    11641 ataaaatggt agatgattta tctgaattaa atgaaagtgg tactatggga tgcccaccag
    11701 aaatacttaa aaaatcttgg gctaaagata tgatttggta tggtccaggt ggaattggtg
    11761 cttcttatac tatacctaga tatcaagagc aacaccagct accatttaga aataatttaa
    11821 aaggaaagac tttcaatggg catgtatgta gatttgcaga aggtaatttt gcttgtttct
    11881 ttggatggcc taatctgact aatgtaccat gtggaggttt tttaggatta ccaggtggag
    11941 aggttaaagc agatatgcaa gttgttgatg tttattatcg taagggcgat aaacttcaag
    12001 aaaactgggt gttaattgat attccttatt ggttgaaaca acaagggctt gatattcttg
    12061 agagaactaa aagtattttt aatgcttagt tatgcatacg tattctaaaa caaaggggga
    12121 tataaagtgg catctaccaa caaaaataat gtaacaagta aagaggaaga aaaagaagtt
    12181 tctacaagtg ggcttgaatc atgtacggtt aaagaaaaca aagctaaaat gaatcaaact
    12241 aattttagta atataaatga aaatcaaaca aatgagaata aaacgaacac atataaagga
    12301 aatatagata aaataaacac aaataaatcc gacataaata aagttaacat agatacagtg
    12361 gatacagata aattaaatac aaatactctt actgaaaaaa ataaaaaagt tatatttaag
    12421 ggggaagata atatgtcatc tacaaaaatt tactatggag attcattaga agtaacgcct
    12481 gttggagtaa tggattacaa tgatttttca aaacagacaa aaagggaaca ggaaatacct
    12541 ggatttgatt ctaagtatag agattttgta gattatatta tgaagattac acataatatc
    12601 tgggaagaaa aaggtatagg tgttatttat gatacatatc acaataatgt tacaatgcat
    12661 tgtggctctt caaatttagt tggtattaaa gatgttattt ccaatactct tcaaacatta
    12721 catgcgtttc ctgatagaag attaattggt caaaatgtaa tatggtctaa ctttggtgct
    12781 gatggttttt tatcttctca ccgtgttttg tctacagcaa ctaacttagg tgatagtaat
    12841 tttggaccag caacaggtag aaaaataaac tttagaactg taattgattg tgccacaact
    12901 aacaatagaa tccacgaaga atggttggtt agagataatt tatggattgt tactcaatta
    12961 ggattaaacc ctcaggaagt agcaaagagt atggcaaagg caagtgaatc taaagtgctt
    13021 agcttacaat caacttatgg tatctgtgaa tctatggatg gtcaatttat gccaactaag
    13081 tatcaagcaa aagatgattc tgttggcgaa atgatgcttg aaatgacaag ccgtatttat
    13141 aattataaat atataaatga agttaaaaaa tactatcatg ataatgcagt agtacatttt
    13201 atatgtgata aagatttaaa tggttatgat gagattcaag gaatgattgt aagtttactt
    13261 gcatcaattc caaatggaag ttatgaagtg gaaagagtaa cttgtaatca aagagaaaaa
    13321 aatgaaggtt atgatgtatc tataagatgg agattacgtg gaataaatga aggtatagga
    13381 ttttttggac agccttctgg aaagcatata gaagtaatgg gaatcaatca ttatcatata
    13441 cttcaaggta aagtaaagga agagtggata acttttgatg gtatggatgt attaaaacaa
    13501 atgtatatgg gagtagaaga ataaagctgt ttagagtata aataattttg aaatgtatat
    13561 agggaagtat tgctttataa aatttaagtg gggaatgtat atgaaaaata gatgtttaaa
    13621 cttttagcaa aaattatgac taaatttaaa agggggaatt ataaaaatat gaaaacaacg
    13681 gttgaaatag aaaatgatat catattagga aaagactatt caaacctaaa aagaggaatt
    13741 gcattttttt caattgcttt aatatacttt ttttattgct acaattttat ggtgggtact
    13801 tttattaaac caacaatgat ttatgcttta gcagatgggg gatttggctt ttcattaaaa
    13861 caaacagaag aaatatttgc agttatgtct tttggtacaa ttccaggtac atttattttt
    13921 ggggtgattt ctacaaaaat tgggaagaag aggactttaa ttagcgttgc tttactgatt
    13981 ggattgacaa catttatacc aatgttatct cctacagata ttatgttatg gaaaatagca
    14041 cgtttgtgta caggagtagt gttgggaggc gtatttggaa cagctatgcc tttagttgca
    14101 gatatgtttc caagtaaata tcgtggtaag ttagctgcta tattaactgc tttattttca
    14161 ttagcaatga tttttggtgg gaaagtatat ggcgttttag gtgatgcaaa ttggcaaata
    14221 ttgatgtata ctgcgattat accacctata gttggagcta tcttagcaat cttctttgtg
    14281 ccagatgatt tggagtatac aaaggaatta gttaaaaagg gaaaagaaac tggtgaaaag
    14341 attagctata taagtatgta taagggaaaa tacttatgga ttggattggg aacgattctt
    14401 ttatcaggtg caaattttac agcatattca gcattttcaa ataatgcaac tacataccta
    14461 gtgacaggaa taggaatgtc agcagcagtg gctgggtcta tatatagttt acaaggaatt
    14521 ggtcaattag taggatattt attctggggt tcaattgcag ataaatttgg aagaaaaata
    14581 ccttttatag gtatggcatt agcagcagta tttgttttta tatatacaaa attaggtgga
    14641 gataatacaa caacattcta tatgatatca gtattactag gtgttgctgt tggatattct
    14701 ggagcatggg gagcatacta cacagagttg tttccaagta aatatcgttc gttgtcatct
    14761 ggtatgtcat ttaatggagg aaggattata tctacgtttg caatacctgc tatagctggt
    14821 acagcttctg gtactttagg tatgatgact atcttttaca tatcaatcgc agtctttgta
    14881 gcaggtgcta ttgtatggtt attcttacca gagacgttaa ataataaaaa ataaaatact
    14941 aggaatttat tttgttactt agaaaggggt ggcttatgat ttcatcaatt gatgtatgta
    15001 ttataatagg atatttgcta ttaatgttag ttattggtta ttattcagga aaagataata
    15061 aaaatcaaga agattatttc ttggcaggta ggtcaatgcc atggattcca attgctttat
    15121 cagttgcagc tactatgatt agtgcaaatg gctttatagg tggtcctgga tgggcatatg
    15181 tagatggaat gtatccagta atgtaaaatg tgcaaaaaat tcttttgtag ttagtgcatt
    15241 tgttatgaat ttagtttact actttatgct attggtagga gtaattttat ttgtttttta
    15301 tgggggaagg acatttgaaa cttcaaatga aataatgatt acttttattt taaatgaact
    15361 tccagtagga gccattggaa ttattattgc aggtgtgttt gcagcagcaa tgtctagtat
    15421 cgactctata ttaaacagta tgacaactgt ttttacgaag gatatttatg agttttattt
    15481 caaaaaagac aacaaagaat catcattaaa agttacaatg attataactg tagttatagg
    15541 agttgttatg acaggtttta ttattatggg atttggagga agcatcaaat caattcttga
    15601 ccttgttggt aactatatct catactttgc tggaccagct acaggtgcat ttgtactagc
    15661 aatgtttaca tataaagcta atgataaagg agtatctatt ggttttattg tagggttagt
    15721 tttaggattt tttatttcta ataaatataa tgtgagttgg ttgtggaatc cagcaatagg
    15781 tgctacaata acattgatat ttggatatat ttttagtgtc attttaaaga aaaatcataa
    15841 agtagatgat attaaaaaat acactgcatt tggtatgaga aatcacctta tttctcaagg
    15901 aatggataaa gtagatggag tatctatagt gccattttct ttaggtaaac aagaactaat
    15961 tgtattagct ttttttatta tccaatatat tatattattt ttgatacaat tttaagaaga
    16021 aatactagat atttaaggag aaatactaaa tgaatcaaaa gaaagttcat tataataaat
    16081 cggctacatc aaaagatgtc gcaagattgg caggagtatc tcaatcatct gtttcaagag
    16141 catttggtag tgcaaatgga aagggcgtaa aacctgaagt aagagaaaga atttttcgtg
    16201 tagcaaacga aatagggtat gttcctaatt tggtagcacg gggaatgatt agtggaaaaa
    16261 ctaatgttat aggtttgata gttggtgata gtctaggtcc attttacaat agaattatca
    16321 atttatttgt agaaaaaatt caagagatag gtaaacagtg tctcgttttt aaagttccta
    16381 gacaacaaag tatagatagt ataatttcaa aagtaattca gtttcaagta gaagctgtga
    16441 ttattacagc ttcggccatg aataaggtga tggctgagac ttgtgaagaa aacaatattc
    16501 ctgtgattct ttttaatcgt tttatacctg gtataaaaat aagtacagtc tatgtagacc
    16561 caattgaagg tgggggcatg gttgctgaat atctgcttaa gaaaggtcat aagaatatag
    16621 gatatattca atttacgaga gaaacaagtg aagaaatgga aaagaaaatt ggattttatt
    16681 ctaagttaag acaaagtggg attcataacc ttaaagaaga atctgcaaac tatgattatg
    16741 attctggata tgaagcaggt aagagaatgt tagcattatc aaatcctcca acagcaattt
    16801 tttgcacaag tgacttgata gctattggtg taatggatgc tgcaaggttt gagtacaagt
    16861 tgagggtgcc agaggattta tcagtgatag gatatgatga tattcaaatg gcttcatgga
    16921 agagttataa tttaacgacg attagacaac cactagattt gttgattgaa aaaacaatta
    16981 acatacttaa gtccttatta aatgaagaaa atccagagtc agtcgttgaa atgttaaaac
    17041 ctgaattgat tgaaagaggt tctacacttt aagtg
//
LOCUS       DAGQWR010000088        17017 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.088, whole genome shotgun sequence.
ACCESSION   DAGQWR010000088 DAGQWR010000000
VERSION     DAGQWR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.088"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(100..624)
                     /locus_tag="MIW95_002545"
     CDS             complement(100..624)
                     /locus_tag="MIW95_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBY3151861.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(689..1441)
                     /locus_tag="MIW95_002546"
     CDS             complement(689..1441)
                     /locus_tag="MIW95_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBY3151862.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDISKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1472..2770)
                     /locus_tag="MIW95_002547"
     CDS             complement(1472..2770)
                     /locus_tag="MIW95_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBY3151863.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQQGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREVNVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2787..5051)
                     /locus_tag="MIW95_002548"
     CDS             complement(2787..5051)
                     /locus_tag="MIW95_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBY3151864.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5306..6124)
                     /locus_tag="MIW95_002549"
     CDS             complement(5306..6124)
                     /locus_tag="MIW95_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBY3151865.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKIIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIIL
                     LDEPTAGLDPVSTRSIVDIIKGLNKDNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFVNENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6117..6815)
                     /gene="cbiQ"
                     /locus_tag="MIW95_002550"
     CDS             complement(6117..6815)
                     /gene="cbiQ"
                     /locus_tag="MIW95_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBY3151866.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIKNIFVLSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKMHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6818..7102)
                     /locus_tag="MIW95_002551"
     CDS             complement(6818..7102)
                     /locus_tag="MIW95_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY3151867.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPDYEPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGVQKGKRS"
     gene            complement(7092..7844)
                     /locus_tag="MIW95_002552"
     CDS             complement(7092..7844)
                     /locus_tag="MIW95_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBY3151868.1"
                     /translation="MKQNIKLGVIAALMLIVLTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIISFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTVTSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8033..8225)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8357..10129)
                     /locus_tag="MIW95_002553"
     CDS             complement(8357..10129)
                     /locus_tag="MIW95_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBY3151869.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYNMDKHPALDSMTRRIVNDIRNIDTN
                     QDMMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10143..11072)
                     /locus_tag="MIW95_002554"
     CDS             complement(10143..11072)
                     /locus_tag="MIW95_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY3151870.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11301..11489
                     /locus_tag="MIW95_002555"
     CDS             11301..11489
                     /locus_tag="MIW95_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3151871.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRAKRRKEVQYE"
     gene            11482..12183
                     /locus_tag="MIW95_002556"
     CDS             11482..12183
                     /locus_tag="MIW95_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3151872.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLLDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSINNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12185..13108
                     /locus_tag="MIW95_002557"
     CDS             12185..13108
                     /locus_tag="MIW95_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3151873.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13265..14188
                     /locus_tag="MIW95_002558"
     CDS             13265..14188
                     /locus_tag="MIW95_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151874.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGCEKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14181..14918
                     /locus_tag="MIW95_002559"
     CDS             14181..14918
                     /locus_tag="MIW95_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151875.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFASIALGRRK"
     gene            14929..15702
                     /locus_tag="MIW95_002560"
     CDS             14929..15702
                     /locus_tag="MIW95_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151876.1"
                     /translation="MWKLLWAECQKIRRSKIVWITFFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            15718..15870
                     /locus_tag="MIW95_002561"
     CDS             15718..15870
                     /locus_tag="MIW95_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151877.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(16243..16851)
                     /locus_tag="MIW95_002562"
     CDS             complement(16243..16851)
                     /locus_tag="MIW95_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBY3151878.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5459 a         2969 c         2145 g         6444 t
ORIGIN      
        1 tttgtctaaa aacataataa catttcactt tatcctcatt tttaagtaat atatattttt
       61 aatttttatt tataactcaa tctagttttt atataagttt tagttttttt ctacatctga
      121 gtaatctatt ccaaaagttt ctacagtaac agacttcata atttgtggag tttgtggctt
      181 atcagctgaa tctgttctaa ctgatgctat ttcatcaact gtatccatac cttctatcac
      241 tctaccaaaa cctgcatact gaccatctaa gtgtggtgag tttttatgca ttatgaaaaa
      301 ttgagaacca gctgaattag gtgccatagt tctagccatt gatatgactc ctctttcatg
      361 ttttaaattg ttagtaaatc cattaccact aaattcacct tttattgagt gacctgggcc
      421 acccattcct gtaccatttg ggcatcctcc ttgtatcata aatcctggta ttactctatg
      481 aaatattatt ccattgtaat atccttcatt tactaatgat acaaagtttt taactgtatt
      541 tggagctata tttggatata actcaatttt gatttctttt ccattttcca tttctatagt
      601 tactatagga tttttatttt ccataattta ttaccttcct tttcattagt taaattacat
      661 aattacattt attatattat actctaaatt aaagtctatc aagtatattt tttcctattt
      721 cgtatccttc ttgataaata gttttacaaa actcatcatc aaagttaagc gtatctttag
      781 gagcaaattg agtattaggt ctaactacta ttattttatc ttcatcatat acttttttaa
      841 tatcctctgg caactcataa ttatattttg tggatattaa aataatttta tccacattat
      901 ccccaattaa tggttcaatt aatgcactcc taattaaacc tccatctaat ttgaaaccat
      961 cataaattcc ttcttgcaca tctttcacaa attgttcttt aaatccaaga gttgcgtttg
     1021 gatttgatgg taacaaccca ctgtatgaaa tgctgataat ggcttcatta cgaggcattt
     1081 ttgatatgtc aacaatcttt tctgatacta ctttattatc aacttcaaca taattaacat
     1141 aaaagttcat ttcttctata ttactatcat ctagttccct taggttgtct atagcaggag
     1201 aattatccac agtattattt actattttta catttccatt ctggatatct tttatatttg
     1261 tccacatttt ctccaagtta tcaacattcc ctgtgtacaa ataatatcca tttattgctc
     1321 ctattgatgt ccctgaaatc gaataaaatt tatttattcc tccatcatac aaggctttaa
     1381 ctactcctgc ttgataagcc ccttttgctc ctccaccttc taaacataat cctaatttca
     1441 tagtctcatc tccaaattgt ttatcttata tttatatttc aaaaaactta ctaaatgttt
     1501 tatatatact atagtggtcc tcaacatttc ctaattctct tatagagtgc attgctaaaa
     1561 ttggacttcc cacatccaca gatggtatat ctatatgagt agaagatatt ggacctatag
     1621 tacttcctcc tctttcatct gacctattaa caaattcttg atactcaaca ttaacttctc
     1681 tacatatatt tttataaata gctatagaat atccatcact agtatatgcc tgactagcat
     1741 ttatctttat aactggacct tttcccatta ttggtttatt agttgggtca tgcttttctg
     1801 gaatgtttgg atgaatagca tgagctaaat ctgatgatat cataaaagat gaataaatag
     1861 actcaaagaa tccctctcta tccttaccca ttgatatgca aattctctcc aatatattta
     1921 acaacatgtt tgaatctgca ccttgtttag tagagctacc aacttcttca ttgtcaaata
     1981 cagccattac atttacacct tgttgacctt tagtattaac aagagcattc aaactagcat
     2041 gtaccattgc aagattatct tgtctcccaa tagatataaa ttcatcatta gcccctatta
     2101 aacttccttt ttcaaattca tataaaaata aatcaaaatc tataatatct tccatattta
     2161 catctaagtt agatgctatt tgctttataa gaaaattatc tttttctaat gtatcattta
     2221 acatcccaac taaaggtaac atatcttttt gcttattata tgaatgacca tcatttatac
     2281 ttctatttaa atgtattgct agatttggta ttatacaaat tggcttttta aagtctataa
     2341 gcctttcatt tggttttaat atactatcac cttttaaaac tactctacca gctatcccaa
     2401 gaggtctatc caaccatgta cttaatattg ctcctccata gcattctgta tttaacttca
     2461 aataagattt ttcagcttct atttctgcat ttggtttaat actaaatgta ggagaatctg
     2521 aatgagagcc tattattcta aatccatctc tttctatatc ttctgaattt actacaaaag
     2581 ctactatagc tgatagattc ttagtaacat aatactttcc accaactctt agattccatc
     2641 tttgattcaa aactaattct tcaaatccat ttttatctaa gatttcctta gtactctcga
     2701 ctgcatgaaa agacgacgga ctatcatata tgaagtctat taagttttta gcaaagttta
     2761 tactatccat tcctcattta cctccattat aaaatattta gtgttgtata ttctttattt
     2821 atttttttaa ttccctgttg gtcaaaagct atagtaacat ctgtaccaat tatagaaacc
     2881 actgtcccaa ccccaaattt aggatgatgt accttagaac caagttttat atcatctgga
     2941 ttagtttctt tattagaatc ttttattgta gcattaactt tatctgcaac tttagatttg
     3001 cttatagtac tcatatactt tttagtatat ttatctaaca tattataact tgctttagaa
     3061 tagcttagtt ctttttttac tttgtacaat ctttctatac attcttctgg cagttcttcc
     3121 ataaatcttg aagctattgc aacatttgtt ttaccatata atgttctctt ctctgtcaat
     3181 gacatatata gctcttcttt agctctagtt ataccaacat aacaaagtct tctctcttct
     3241 tctatttgtg aatcactcat agattttata gctcttgata taggaaataa cccttcttcc
     3301 atacctgtta aaaacacaac agggaactct aaacctttgg atgtatgaat tgtcataagc
     3361 gaaaccctat cattgtcttc ttcatcattt gattcagatg taagtgctat acttgttagg
     3421 aatgtctcta agtctttatt ttcataagta tcatcattac tactttcaaa ctccaaagct
     3481 atagaaataa attctctcaa attatcaatt ctgtcttgag cttcttcacc tttgcctgtt
     3541 aaatcttttt catttttatt tcttatctct acaagctcgt ccatatatcc tgtagtatcc
     3601 aagacttttt ctataagttt gctaacagga taaacttctt ttatagttct aagagtccct
     3661 ataatatcaa caaatccact tatacttgcc cttgccttag tagatatatc tgaatttgtt
     3721 tctatatcaa ttaatactga gtatatactt tcctgtttta agttagctct atcttcaatt
     3781 ttttctatag ttctaagccc tatacctctt ctaggaacat ttataatcct ttttatagat
     3841 atatcatctt gtggattttg aataactctt aaatacgcca acaaatcctt tatttctttt
     3901 ctctcgtaga attttgtacc accatatata ttgtatggaa tttgacttct atttaaagcg
     3961 tcctctacag gtcttgcctg tgcatttgct ctataaagta ctgcaaaatc cttataaggt
     4021 ctattttctt ttctagctat ttgtgcaatt gaatcagcaa tgaaatcagc ctcttctatt
     4081 tcgctaccag ttaattgtat ctttataagt tctccttctt ttttttcact ccaaagcttt
     4141 tttctttttc tttctatatt attagatata actttattag ctgcatctaa tatcacttgt
     4201 gtagacctat aattttgttc taactttaca acatgtacat tgtcataatc tttctcaaat
     4261 tcaagtatgt ttcttatatc tgcgcctctc catccataaa tactttggtc atcatctcca
     4321 acaacacaaa tattttgatg ttctcttgct aataatttta tcaattcata ttgagcttta
     4381 cttgtatctt ggtactcatc taccattatg tatctaaatc tacttctgta atatgctaat
     4441 acttcatcat tagccttgaa aagttctaca gttttaagta ttaaatcatc aaagtcaagt
     4501 gcactatttc tttttaaacg gtcttgatat aacgcataaa tatctgcaat cttaaccatt
     4561 ctattatctg ccatgttgat atctttaaac tgctttggag tataaagttt gtcttttgct
     4621 cctgaaattg cacttataac tgcttttggt tcaaacactt tatcacttaa atttaattct
     4681 tttagacagt ctttaaccaa tgtaacttgg tctgctgaat cataaattac aaaacttcta
     4741 ttgtaaccaa tcttatttat atcttttctt agtatacgaa cacagcaaga atggaatgta
     4801 cttatccaca tatccttagt ttcaggacct atattttgtt caactctctc tctcatttca
     4861 tttgcagctt tgtttgtaaa tgttatagct aatatatttg gtgcttgtac acctttatct
     4921 tctataagat atgctattct tgttgttaat accttagttt tacctgaacc agcaccagcc
     4981 agtataagta ctggaccttc agtcttttct actgcttctc tttgagcagg atttaatgta
     5041 tctaaattca tatttctcct cctgttaaca gccataaata tattttaatc taaaatatga
     5101 attacttcaa attataatta tgtattatat taatagatta tttccaatat attacaatta
     5161 aataacattt ttccatataa tttatttaat tgtttaataa aaaaaataac cttatgattt
     5221 ggggaatcat aaggcacctt taaggggtct aggttaaggg ttattatata taaatctatt
     5281 tttttaaaat aggtttaggg cttaattatt tatttgtatt aagttctctt tccttccaat
     5341 acttatacaa atcttcttct ttcttaaata agggtaaatt catatttcta tgtactttta
     5401 ctaaccatgg ggattctagg ccagcttgta taatgttatt ttcatttaca aatacatttt
     5461 ctgcctttcc ttcatcaatt aaaatcccct tatccaatac atatatataa tcgcatattt
     5521 catacattaa attcatatca tgactggata ttactatttt tatattatct ttattcaatc
     5581 cctttattat atctacaata gacctagtac ttactgggtc taaaccagct gtaggctcat
     5641 caagcaatat aatttcattt tccattgcta taactgatgc tatcgctacc ctttttttct
     5701 gaccataact taaaaaatgt actggtctat ctataaaatc tattccatta acagctacta
     5761 gagctttgtt tattctctct ttaattattt tttcatctat accaatattt ctcattgcaa
     5821 aggctatatc atcatatact tttgaataaa aaatttgttt ttctgggtct tggaacacaa
     5881 tacctacatt ttttctaagg ttgtataaac ttctcttatc atattttaac ttttcttctt
     5941 taaaaagaat ttctccagat gtaggcttta atatacccat taaattcata aataatgttg
     6001 attttcctga accatttgaa cctattatac ctataacatt tccctttgaa aaatccatat
     6061 taatatttaa tagtgcattg gtattctttt catattgata agttaaatta tttattttaa
     6121 acatcattat cccctactat gtgaaattta ccatcataca attttatgtc taatgatata
     6181 cacatatcat cataccttgt cataagtctt ttgtacagca tactgccaag aattcctaat
     6241 gatttatatg aagtttttaa atttatgtac ccaaatctta gttcttgtga cttatatatt
     6301 tctgaaaatt cttctaaaaa tatgaatata aacctatata ttaacattga tagctcaaca
     6361 aaagtatcag gcaaatgcat ttttttaaag aagaatatta attgattaaa aggtgttgta
     6421 agtatacaaa agtatacaca agtcaaacaa gacatagctc taaataacac atgaatagac
     6481 atgtcaatag atgcttttga tattcctaca ttgaaactaa aaatttcaaa actatataat
     6541 aagtctgcct tattaaaaga tatatttaca agagttattc ctatactaag aaataaaaag
     6601 tacattggta ttctgagtaa ttttaaataa ttctttaaat ctattccagc tacacaaaca
     6661 actagaatac tcattaaaat cataattcca cttaaaacaa atatattttt tattaccata
     6721 gaagcaatca gaaagattac tcctatggct acttttttat ttggatttac tttacttaat
     6781 ctatttgtat atgcatattt atcaattata agcataatta acttctcttt cctttttgaa
     6841 cacctaatat gtaccctatt acaccagcac ctaacgctgc ttgtgcagag aataataaac
     6901 tttctatttc tccacttggt ggctcatatg ggctactaaa ccatggttca taatctggat
     6961 ttatcttagt aatttcactc tcagcttgac catctgctcc tccatattca gctcccgaat
     7021 taacaagtaa tgggaatatg ataagagcta ctactaataa aagtagtaat atattagttt
     7081 tcgtctttgt tttagcactc attagcttta actcctttat cattattttc tagtatgctt
     7141 ttttctccat tttctgtaat taaattatat acaattacag taagaatacc ctctgctatt
     7201 gctattggta tttgagtcat aaagaaaatt cctaaaaact taattgctga agccatcact
     7261 ccacctgatg ggtctgggaa agccagtgct aactgtatcg aagttactgt atatgtagcc
     7321 aaatctccta ttgctgctgc aagaaatact ggcattgcat tatttccgtc ttttttcttt
     7381 aatgctttaa atattaagaa tgatataatt ggtcctataa tagccattga aaaagcatta
     7441 gctcctaaag tagttattcc tccatgtgcc aatagtaatg cttggaatat tagaactata
     7501 gttccaagaa caaacattac acttggtcca aacattattg ctccaagacc tactccagta
     7561 ggatgtgaac aacttcctgt tactgaaggt atctttagtg ctgacaatac aaatacaaat
     7621 gctccacaca gcgctaataa tactttcttt ttagggtctt gctttactat ttttcctatt
     7681 gattttagtc ctactaaaaa aaatggtata aatataactc cccatgctat agaccatttt
     7741 actggtaagt atccttccat tatgtgcatt gcattcgatg taactggagt taaaacgatt
     7801 aacataagtg cagctataac ccctaatttg atattttgtt tcattttact atcccctctc
     7861 tccctttcta ataattaaaa tataaataat atatgtctga tttattttaa aaataaaaat
     7921 aaaaaagcct aactcgatgg ctaggcttct aattttcgat aatccaaata caaacacata
     7981 aaatgaacat atctgtataa ttattataag aaactttctc catccccgaa gaaattactt
     8041 taatatttta ggcaggtctc ctgacttagc ttcttcctac tcctcttcct tcccatgaga
     8101 tataaaaaat atcatcatag tggctaacaa aagtttcgtc tgcatcacag tagcgggggc
     8161 tgtaaaggaa tttaaccttt ttccctatta atcttgctta aagaacctat aatattatat
     8221 atttaatttt taaattattc ttaaatcatt ttctatataa atcattgtat accttttctg
     8281 tgaaaatttc tacatttttt ataaatttta ttcattttta tatttttatg tatataaatt
     8341 cttttttgta agtttattaa tttctaatta tcttctttaa atacattgaa actacatcag
     8401 aaaatctagt tatatctctt gaatatataa aatcatttcc atatattaat ctttcagctt
     8461 caagttctga ttcttttcct gtaaatactc ctagtactaa tatattttgc tttctagctt
     8521 ttctcacttc attggctgta tctctcaaac caactatacc cctataagcc ttttctcctc
     8581 ttaatgttct ctcactactt tttcctatct ttacatcatt tggtcttcca tcgctgagaa
     8641 ctatcaggat tttattttct tcatctctat ttattagacc actacatata gattttattg
     8701 ccaaaccatc tctattatta ccagcacaaa aatattcaaa tatattttca ctatttttta
     8761 tactatcatc ataatcttta aatcttttta atattgtata gtctaaaaaa ctagaaaatc
     8821 ccatcactct atttggtatg ccaacttctg ttagagcatt tgcaattatg taagcttgaa
     8881 ttgccacatt tccctgattt ctactttgag agccacttgc atccaataat atatcaataa
     8941 cgtatttccc tttttcattt tctatatctt tataaaatat tttattatta ttacttctac
     9001 caactctcca agctctattt gctaatatag taccacaatc agaatatact ctacttttag
     9061 cattttcttc tattaaaatt cttgacagac tttcttttaa tctctttatt tgctgtctat
     9121 acattctagc atgttctcta aattttccta aattattttc tttttgtctt gttacatatt
     9181 ttagttgaaa tacattatta cactcacttc taagaactcc atctgtaaag tgtattctac
     9241 aaccttcatg aacatttcta caattttgtg tctcaatttt tttaatttca cctttactca
     9301 aataagtttt tccataataa tgttctattt ttgaatatat cttatttgca gtgtcttcat
     9361 caacatatat aactcggtct gctgaattgc tacatttaga atcatcaaaa tcccctacac
     9421 cttctacaag catggatgaa gatattgaat ctatatcact ttcaataata ttttcttctt
     9481 catcactata aagttcttca tacatgaaat cagcaaatgt atcaaaattt acttctacac
     9541 tttctacatt attttcatca ataagactat tttcttctcc atttaatatg tattcgaaat
     9601 aagttaaata tataacatct aatgctttta aaatctccat catatcttga ttggtatcta
     9661 tatttcttat atcattaact attcttctag tcatactatc taatgctggg tgcttgtcca
     9721 tattataaag tacaaatgtg tatttaattt tatccataat attatcattg tgtattttgg
     9781 taaaacgtga aacaatatct ttataagcat ttcttcttat gtcttctatc cctggcctat
     9841 ctttaatgac cttttcttca actattgtat ttaatataac ttgaattaga cccagtataa
     9901 tatctttgtt gtatcctttt cttattctat ttgtaagata ttttttaact atatcccaat
     9961 ctacatactt tcttctagca cctgcaatta ttgcaaaata tagacctatt tcttttgaag
    10021 agtaatcttt ttctgatata tctatacttt cattgtaatc accagctatt gtccatgata
    10081 agttattagc tctattttca aattcataat catcatatat ccatttcata atctcaccta
    10141 tattaaatat gacttattgg aaatacatct atactctccc atttattagg tattctagtt
    10201 ttaattacat ctccaaccat ttctttctca tatacatcaa aagtctttcc tgtaagtccc
    10261 atattaattg caagagttgg ttttaatcct cttcttatag tcttaagaga tgccataaga
    10321 ccacgtaaat caatagcttt actagatatc tctccatttt gagacttcaa ttgtaaatct
    10381 aaaaatattc ctgcaaagtg tattaatttt tcttcatcag catctgaaaa ttcatttttt
    10441 aaaatcatca ttaacttatc ttcttctata ggaggaatat ctattaccat aaatctagat
    10501 accagtgctt cattaagttc ttttgttcct gcatattcat aattcatagt cccaataaat
    10561 cttgttgctg gatgtagatt gactctctca tacccaggta catcaattat tcttctatag
    10621 tctaaagctg aatgtaccac aactatagca tcattttttg ccatattaat ttcatcaaat
    10681 actccaaaac ctccattaat agcacactca taaactgagc ctcttctaag tttaacttca
    10741 ttgtctataa atgtatctgt acctataagt gttgaactgt ctgtatttat atgaaatgat
    10801 gtattccact gaggtctatt aaatatttcc cctagattat cagcaagtaa attttttccc
    10861 gttgcctttg gtcccgataa taatatattt tcttcttcta aaatagctgc tatacacata
    10921 gacaagatat ctttaccata aaaataagtt ttagactttg ttactctgta ttctaaatct
    10981 ttttctaaac cataataatt tctaaaatac agtacatctt caataagttt ttcatctacc
    11041 ccttgcaccc ttagattatc tattatatcc atatttttac cccttttata taaatagtat
    11101 ttgtttcatt tcttatcacc attttagcat aaaaggatat aagctaaaat tgatttttta
    11161 atctccccca ttttaagtaa aaccatgcac cattttatca ctacaaaatt aagagtacta
    11221 cttactgata tttcttgatt ttgttcgtct atacgtatat aatatatgct gataaattat
    11281 gaggaaagga tgattgaaat atggaatata tgtcttgccc agaagtagca aaaaaatggg
    11341 gcatttctga aagaagagta caaaaactat gtgaagaaag tcgcatacca ggagtttcaa
    11401 aactcgggta tatgtggctg ataccaaaga acgcagaaaa gcctgttgat ggtagagcga
    11461 aaagaagaaa ggaagtacaa tatgaatagc gttttgatta tagatgatga caaagaactt
    11521 tgtgctctta tgaaaaaatg tgttgagcaa gaaaatttat ctgctattac tgcacattgt
    11581 ggtgcagaag gtctacattt gctcgataaa aatagacaca cactctctct tgtaatttta
    11641 gacataatga tgccagatat agacggtttt caagttctga aaaaaattag agaaacaagt
    11701 aatattccag taattatgct tactgctaaa aatgacgagg atgataaagt ttccggacta
    11761 cgtttaggtg ctgacgatta cctaacaaaa ccgttcagca taaacgaact tatggctcgt
    11821 gtcaattctc ttattcgtcg gtatactatg ttaaaccctg cttttataac agacacagat
    11881 tgcataataa taaatggcat gattattgac aagacaaacc gaattgtttc tatcaataat
    11941 attcctgttc aacttacaag caaagaattt gatttacttg cttttctggc ttctaataag
    12001 ggacgggtat ttaccaaaaa acagatttat acacaagtat gggaagaaga atatgctttt
    12061 gacgatagta acatcatgtc tttcatcagt aaattgagaa aaaagattga accagacccc
    12121 aaccatccat tttatatttt gacagttcgt ggtgtcggtt atcgttttaa taaggaggct
    12181 taacatgaat acaagtgtct atctactttt gatattggct cttagcctga ttattatagc
    12241 gtaccttgtt tctgtcctgc tacgtgtccg aatacaactt acacttataa aagatgcttt
    12301 agaggatata aaaaatggaa accttaatcg ccgtatttta acagatgaaa acgacattac
    12361 aaaacaaatt tgctatgata ttaacgaaat agcaatcaat agccaatttc aacttattca
    12421 gcaaaaacaa tcagaacaag cctacaaaca gctaatgaca agtctctccc atgatgtaaa
    12481 aactccactt gcttctttgg tcggttattt ggaagcagta gaaagtaaaa ttgttgtagg
    12541 agaggaaaaa gacgagtata tccatgttgc gtctaataag gctcattact taaagaattt
    12601 tgtagaaaat ctatttgaat gggtaaaatt ggattctaag gaacaagttt ttcattttga
    12661 tatttttgac ttgaatgaat tatcccgtaa tattatatct gattggatac ctgttttaga
    12721 aagcagtcat tttgaatatg aatttgatat accagaaata gagtacttct tacgtataga
    12781 cgcaaatgca tatacccgta tcattaataa cctattacaa aatattatta cccatagtag
    12841 tggcaacaaa atgacattga gaatttttga aaacaaagag caagctcaaa ttataataac
    12901 agataatggg aaaggtatat cttcagacaa tctcccacat atttttgaaa ggttgtacca
    12961 atgcgaccat tcacgagctt caaagggaaa tggtttaggc ttggcgattg caaaggaact
    13021 tattaacgct cataagggaa atattaccgc caacagcact cccggtatgg gaacagaatt
    13081 tacaatattg cttcctaaag ccctgtaaaa gtacgaggct ttcttactct aaaacaagat
    13141 aaaagcaaga ttatggcaag gttttggcaa ggttcatgtt ttataattct aattgtagtt
    13201 gattttgacg ttgtatcaac tatcttttgt agaaacaaat ttattaaaag tgaggaaatt
    13261 atatatgaat gattttgtga ttgaaacaaa gcaacttaca aaaatttatg ggaatcaaac
    13321 agtagttgac aaagtcaatc tccatgtaaa aaaaggccga atatatggtc tgttaggacg
    13381 caacggagct gggaaaacca caattatgaa aatgatttta ggattgactt caatcacgta
    13441 tggcgaggta gatgtatttg gagagaatat taagggatgt gaaaaacgag tatatccccg
    13501 tatcggtgca attatcgaaa cgccaggatt ttatcctaat ctgacaggaa cggagaactt
    13561 ggagattttt gcaaaacttc gtggtacagc cgcccctaat tctgtaaaaa gagcattgga
    13621 gattgtaggc ttaccttata aaaataaaaa gctgtttagt aaatattcac ttggcatgaa
    13681 acagcgactt ggtattgcca acgctatcct gcatgacccg gaactgttag ttttagatga
    13741 gccaaccaat ggacttgacc ctattggtat tgcggagatg agaaagttta ttagggactt
    13801 gagcgttgag catggaaaga caattcttat ctccagtcat attctttcag aaatctcatt
    13861 gttggctgat gatattggaa ttatcgacca cggtgttctt ttggaagaaa gtagtatgag
    13921 tgaactagaa aagaaaaaca gtaaatatat cctgctacaa gtttcagata ttccaaagac
    13981 atcacttatt ttagagcgtc aatttaacac gaaggattat tccgttcaag atgaccacac
    14041 tttacgcctt tataacacta ctctggatat gggtgaaatc aataaagcac ttgtaatgca
    14101 gaatgtaact gttattagtt ctcaactttg taatgatact ttagaggact actttaaaaa
    14161 aatcacagga ggagagggca ttgcttaaac ttattcaaac agaatttctt aaactgcgtc
    14221 gtaggaaact tatttggctg atgttgttgt ctgctctcat tatgccgttt ttcgcactac
    14281 tatacttcaa ttattttggt aaaacaggtg ttgagccaac aaagttttat aaatggtctg
    14341 catttggtta tacactttgg attattttgc ccgttgtttt gggtatattt tgcaccatgc
    14401 ttatacacga agaaaaccag tatgatatgc tcaaacagct ttggattgta cctattagta
    14461 aaatgggata cttttttagt aaatttttcg ttgttttgat ttactctatt tgttttatgc
    14521 tgattacagc cgttgcttct gtgttattta gtgtactttc tggatatgtc gtatttagct
    14581 gggcaagcat tttatatttg ttgaaaaaat gtttagaaat tggtgtaatc actgcttttg
    14641 taatgttgcc gattttggca atcgctacat ctcaaaaagg atacattctc ccaatttgta
    14701 tcacattggt atatgccttt tccggttttg ttataatgac aataaatatg tatttacacc
    14761 cattatccag tatgggagtt attatcatga gaaataaaga cattccaggt cttatatttt
    14821 cacaggaaat taatgtccca ttagcatttc tttgtatttg tatttggagt attgtatcag
    14881 tattgtttgc aagcattgca ctaggaagaa gaaagtgagg tgtagataat gtggaaatta
    14941 ctttgggctg aatgtcaaaa aattcgtcgt tcaaaaattg tttggattac attttttgca
    15001 tccattatga tagcagtcat agtttttgca ggagggcagg atgtatataa tgacccagat
    15061 ttacattatg gcttaaagag cgtttatgac ggttcacggt atatagacaa tgcaggttgg
    15121 tatatggacg cggtacaacc ttggtcaact ttttttgttc ttcctgctgt cattgcccta
    15181 ttaggaagct atattatttg tcgtgaagaa gaggaggaca ctattaaatc cctgcgtctt
    15241 attcccataa atgaaacaaa attaaccata gcaaaaatga ttattacttt tatattcagt
    15301 atacttcttt atttgttact ctttactata acatttttga ctgaatccgt tttacatttt
    15361 tccgatttat cgacaaaact ggttttaagt tgcatgaaag agtattttct tgatggcata
    15421 ggtgtattct ttgcaatttc tcccattatt gccttggtat cgcgcatgaa aaaaggatat
    15481 tggcttgcat tagtattcac agaagtttat tctattgctg gactatttgc gggtatgtca
    15541 aatgtcttac aaaccttttt tcctattgca gcaatattta atatttctgg atactatata
    15601 actactggag aaaaaattat ggggagtatc ataagtttat tactttgtgc ctgcctttct
    15661 gcatttatac tgaaagggct aaaacacaat gaaaaaaatt gaaataggta tatcaaaatg
    15721 aaaaagataa aaaaactgat tattctaatt gtattcttgt ttctttttgg catgttacac
    15781 tgttatactg aatggaaatt caacaatgac ccacctaaag gggtaataga aagaataaat
    15841 gagtggactg ataagattgg actggattag taacttatgt taattatagt gaatgtaata
    15901 ttagcaagtt attgtttcgt attttaatac tataacttta aatatttggt tttaatcttc
    15961 cgaacgaata taaaaaaact gtcacagagg caacatattt ttgaatattt ataaagtggt
    16021 agttttgatt ttcaaagctg ccgccttttc ttctatataa gcaaacttat cctcaacaaa
    16081 gtgtaaaaca tggtacattt taagaaaaaa gtaccacaga cacaagcaat aaaagaaacg
    16141 ccgagaacct acaaaaacgt agtgcttatg cagatttacg acgccttcta ttttctcaac
    16201 cgtcttaatg accttgtaca aaagaaattt ttctaatatt ttttataatc ttatcttgtg
    16261 atgtttttta tgtataaatg acttaataac aaatccaata aaaattatgg atattggtat
    16321 aattagaacg aggaatttat aatcattata atgtgacatt attcttaccc aactctcaga
    16381 aaaaatatat cctatactga ttaatgaagt attccatatg gttattccca cgatagatga
    16441 tattacaaaa gttataatat tttgacctgc tatacctgct ataaaagata tgtatgttct
    16501 acataaaggg attaatctac atacacaaac agaaatagaa gagtatttat taaaataatc
    16561 ttgagatgca aatattcctt tctttgcctt tggacatatc tctattattt tatttatgat
    16621 agatttacct ccccaatttc ccactgtata acaaaatatc gaaccaagta tacctgcaat
    16681 tatacttaaa attaaaattg tcagaaatcc aaaatgattt cttgcagcaa ttgctccaca
    16741 taaaggtaaa acaacttcgc taggtaatgg aaaacaagca tactcaatca taattaatac
    16801 aaatacagag attaatccat attgtaaaat aaaactattg attacagaca cttacttatc
    16861 tatctccttt cttatttaaa ggttttaagg gtacatattc taaggattct caccctatat
    16921 ttaaagaatc aatctcttta aactattata tgtatttaca caaagcattt ataacatact
    16981 ttaaaaatag taccttaaaa taagaaactt aataaat
//
LOCUS       DAGQWR010000089        16989 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.089, whole genome shotgun sequence.
ACCESSION   DAGQWR010000089 DAGQWR010000000
VERSION     DAGQWR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16989
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.089"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            40..282
                     /locus_tag="MIW95_002563"
     CDS             40..282
                     /locus_tag="MIW95_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBY3151879.1"
                     /translation="MQNSMKQEPCDFSHGRFRFKTSDNRVLIPVVIFLGSIVTALCDL
                     ISRVILSPVELPISTITSLLGAPIVVMLLVKRKDRA"
     gene            285..1481
                     /locus_tag="MIW95_002564"
     CDS             285..1481
                     /locus_tag="MIW95_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151880.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(1601..2326)
                     /locus_tag="MIW95_002565"
     CDS             complement(1601..2326)
                     /locus_tag="MIW95_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBY3151881.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGANFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTPFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            2804..4903
                     /gene="nrdE"
                     /locus_tag="MIW95_002566"
     CDS             2804..4903
                     /gene="nrdE"
                     /locus_tag="MIW95_002566"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBY3151882.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYLKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            4916..5887
                     /gene="nrdF"
                     /locus_tag="MIW95_002567"
     CDS             4916..5887
                     /gene="nrdF"
                     /locus_tag="MIW95_002567"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBY3151883.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKKMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            5963..6589
                     /locus_tag="MIW95_002568"
     CDS             5963..6589
                     /locus_tag="MIW95_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBY3151884.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIIEIPEQKFIMLKGSGDPNSKEFI
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            6984..7613
                     /locus_tag="MIW95_002569"
     CDS             6984..7613
                     /locus_tag="MIW95_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151885.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            7614..8396
                     /locus_tag="MIW95_002570"
     CDS             7614..8396
                     /locus_tag="MIW95_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151886.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAINTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            8389..9267
                     /locus_tag="MIW95_002571"
     CDS             8389..9267
                     /locus_tag="MIW95_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151887.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIIGKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIKIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSEYYI
                     KLKKDILMTMKSNKDI"
     gene            9300..10259
                     /locus_tag="MIW95_002572"
     CDS             9300..10259
                     /locus_tag="MIW95_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY3151888.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDVALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            10573..11265
                     /locus_tag="MIW95_002573"
     CDS             10573..11265
                     /locus_tag="MIW95_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3151889.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            11275..12279
                     /locus_tag="MIW95_002574"
     CDS             11275..12279
                     /locus_tag="MIW95_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3151890.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGIHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKM
                     "
     gene            12527..13378
                     /locus_tag="MIW95_002575"
     CDS             12527..13378
                     /locus_tag="MIW95_002575"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBY3151891.1"
                     /translation="MSLDVIFILKSVIIAIVEGFTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            13482..14228
                     /locus_tag="MIW95_002576"
     CDS             13482..14228
                     /locus_tag="MIW95_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151892.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            14228..16144
                     /locus_tag="MIW95_002577"
     CDS             14228..16144
                     /locus_tag="MIW95_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151893.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTYFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLIYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            16423..>16989
                     /locus_tag="MIW95_002578"
     CDS             16423..>16989
                     /locus_tag="MIW95_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBY3151894.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYKSEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKL"
BASE COUNT         6687 a         1678 c         2954 g         5670 t
ORIGIN      
        1 ggggatggcc tgtggagaat tagtaagaca tattttagta tgcaaaattc tatgaagcag
       61 gaaccctgtg actttagtca tgggaggttc agatttaaaa catcagataa tagagttttg
      121 ataccagtag taatattttt aggcagtata gttacagctc tttgtgattt aatatcaaga
      181 gtaattttat caccagtaga attaccaata agcacgataa catctttatt aggagctcca
      241 attgtagtaa tgttattggt gaaaaggaag gatagggctt agacatgttg aaaactaata
      301 atttaagtgt aggatacaat aacaaggtag ttataagtaa tataaatgta gaagtaaaaa
      361 atggagaaat actatgtctt ttgggaagta atggagctgg aaaaactacc ctattaaggt
      421 ccttatctaa acttatatca ccaataaaag gtgaaatata tttgaatggt gtaaacataa
      481 attgtatatc aagaaaagca ttatcaaaaa aaatggcttt agtacttaca aatagattat
      541 taggtgattt aatgactgta caagatatag tcaatatagg aaggtatcca tatacaggat
      601 tttttgggag tctatctaag aaagatttaa taatggtaga tgaagcattg gagtctgtag
      661 atgccttaca tttaaagaaa agatattttg atgaactaag tgatggagaa aaacaaaagg
      721 tgttggttgc aagagctctt gttcaagaac cagaaataat tatattagat gagcctacaa
      781 ctcacttgga tataaaacat agattggagc tcataaacat actgaaaaaa ttaagtaaag
      841 aaaagtcaat atcagtaata ctttcattgc atgaaataga catagcatta aaaagttgtg
      901 ataaagttgc tcttataaaa aataataaag tcatagctta tggtcagcct gaagatgtag
      961 tagatgagga tattataaat tcgctttatg aattggatga taaaaacttt aatagtcttt
     1021 taggttcagt agaaattagt aataaatcta aaaatgaagt ttttataatt ggtggtgggg
     1081 gtaaagcaac accaatttat agagctttta ctaaaaaagg tataggtctg tattctggaa
     1141 taatacatga aaatgatatt gattatgaaa ttggtagaac tatggggata aagatgttta
     1201 cagaaaatcc atttgagccg ataagtgatg agagtttcga tttggctatt agaaatttaa
     1261 atgattccaa gattataata gatacaggat ttagtgtagg agagacaaat aagaggaata
     1321 tagatattat aaaagaggct ttaaaactgg ataaaaaagt ttattctttt agaaatagag
     1381 atgagagtaa gaagtattat gacagtttgg atagtaagat agaacatata gataaagttt
     1441 ctcaaataat caatagtgca gatataaata atttattata atttatgaaa taaaatgtac
     1501 ttatgtgcta tagaaaaaag agtatagatt ataatttttt tgaaattaca atctatactc
     1561 ttttgttatt ttgtttatat atttataaaa ctttagcatt ttatagataa ccaatagctt
     1621 cctctctagt taaaaaaaag tgaatccctg tagtcgattc tttccatcta ttttcattaa
     1681 aatcttcagc tacaatcgtt tctcctactc tatatataaa attttcatca gcatatgcac
     1741 gagcactttt aaaagattct ttaccatcta tactagtaat tgacagtact tttgccttat
     1801 cacatctaca agcatttgat gtagcagaca ctctttttgc gtcgctgggt attaataatt
     1861 gtaccattct gaaattaaaa cattttttat agcctataaa tgctcctgtt tcaggacaat
     1921 acaatttaaa aaaaggggta ctttcatctg atattactcc atttaaagta gcatgttcta
     1981 aattggaatt ttctaaatta gctcctctaa tatctgagtt agataagtca caaaatctaa
     2041 aatttgcacc acttaagtct gcatttttta atatagaatt ttttaatgaa caatccgaaa
     2101 gataagaatt attaaaaata gagttttcta aatctgcttt ttctaaattt aaattgataa
     2161 ggtcagacca cgaaaagtca atattactta agcagtatcc tcttaagtcc atattttcta
     2221 atcttaattc ttttaaatct gccttttctc cagcattatt gtttttaagc cataaataat
     2281 gtttctcaag tgttttttct aattcttgtt tagaaatagt tttcattaaa atcaccttca
     2341 taaatttatt tttatatatg tagtagcaaa atagtcatat atttaattat ataacaataa
     2401 ataaaaaact tctaaataat atataaaata tcatcttgaa agtacttttt tcaatatatc
     2461 agaatatatt tttaagctat ttagagataa tttaaatttt taaacaggac ataataagct
     2521 tgtatttttt tatatttgag ttgataaaaa tttctttaaa taaaaataat gtcaattata
     2581 aagtaataaa agtttagata taaaaaaatt tgtgtatata ctatatgtag tatataatag
     2641 ataaagcact gactatatgt agtgcttttg tttttgaaaa atacttattg catttctata
     2701 attatagatt aattttggga tttcaactga taagtaccaa aagaaataat aagcaaatta
     2761 agtgaaatta tggcacaaga ttatgttaaa aggacggtaa ataatgaatt ggatagagct
     2821 taacaatcaa gtgataatta aaaatgaaca aggtaagtac caattggaaa aggataagga
     2881 agccttaagt agttacatag aagaatttat acaaccaaag ttgagaaaat ttaataacct
     2941 agaagaaaga ttgaagtatt taatagatga aggttattat ttaaaagaag taataaatca
     3001 gtatagcata gatgttatta aaaatatata tgatatgatt gataaacata agtttgaatt
     3061 tcaatcatat atgagtgcaa ataagtttta tcaaaattat gcattaaaga gcaatgatgg
     3121 aaaggaaata ttagaaacat ataatgataa agtgttaatt gtagctttga cactaggaaa
     3181 tggcgataaa gatttagctt taaacttggc agataaaata gtaaaacaag aatttcaacc
     3241 agctacacct acttttttaa atgctggaag aaagagggct ggagaaatgg tatcatgttt
     3301 tttactatct gtagaagata gtacagaagg tatatcatat gctatatctt catcaaatca
     3361 cttatcaaag attggtggag gagttgcact aaatttatct aaacttagag catctggcga
     3421 atctatcaaa gatatagaag gtgcagctgg aggtgttgtt ggagtagcta aaatgttaga
     3481 acaatcattt agttacttta atcaaatggg agctagacaa ggttctggtg cggtgtacct
     3541 tactgtattc catcctgatt ttgaattgtt gatggatact aaaaagataa atgcagatga
     3601 aaaaataaga cttgctacat tatcattagg agctataata ccagataaat ttatggagtt
     3661 ggcagaaaaa aatgaagtag catatgcatt ttatcctcat actgtgtata agaaatatgg
     3721 agtttctctt gatgagatag aaatggatga gtggtatgat aaattggtta atgatagtga
     3781 tataagaaaa aaagaaataa atcctaggca gatgttgaca aaaatagcac aaatgcaaca
     3841 agaaagtgga tatccatatg tagtgtacat agatacagct aatagagaac atacattaaa
     3901 agatgttgga attattaaaa tgtctaactt atgttgtgag atatttcaat atcaaacacc
     3961 ttcagaaata gaggggtatg gaggaaaaaa tgaatgggga caagatataa gttgtaacct
     4021 aggttcttta aacatagcca atgtaatgga taataaaact atagaaagta cagttgaaac
     4081 agcaataaga gcactgtcat ttgtagcaga tagcacagat ataaaaccag tgccaacggt
     4141 aagcaatagt aacagcaagt ctcattctat aggattagga gctatgaatt tacatggata
     4201 cttagtaaga gaaaatattt tatatacttc agatgatgct atagattttt ctaatgtatt
     4261 ttttgcaatg gttagatact attcaattaa agcttctatg aaaatagcca tagaaagaaa
     4321 tcaaactttt gaaggatttg ataaatcaga atatgttaag ggaagaaata gcaaggtttt
     4381 atcaaaatac tatgaacaat cttatctacc taagtcagaa aaggtaagag cattatttga
     4441 agggatttat ataccaacaa aagaagattg gacaaaatta ttggatgaag taaaagaaaa
     4501 agggatatat aatgcatatt taatggcagt agcaccaact caaagcatta gttatgttca
     4561 aaatgctaca tcaagtataa tgccaattac tgagccagta gaagtaagaa catatggaga
     4621 ctctactaca atatatccaa tgccattttt aacaaatgat aatatgttgt attatcaatc
     4681 agcatacagg atggatatga gaaaggtcat agatttagtg gcaacagttc aaaaccatgt
     4741 tgaccaaggt atatcaacaa cactgtttgt tacagatgaa aagacaacta gagatatagc
     4801 tagacattac atatatgcct ataaaaaagg tttgaaaagt ttatattaca caagaacaaa
     4861 gatgacaaga gatactcatg agtgtctagt gtgttcagta tagggagaga aatatatgac
     4921 atttaattta cataaaatac ataatgcagt taactggaat agagaagaag atggatttac
     4981 acaagctttt tgggaacaaa atgtaaaaca attttggctt cctgaagaaa tatctgtttc
     5041 taaagacata aaggtatgga atgaactaag taataaagaa aaggaattat ataaaaaagt
     5101 attgggtggt ttaacattac tggatacaaa acaggggaat aatggaatac catctatgat
     5161 gagtttaaca gaaaacctac aaagaaaagc tgtcttgtct tttatgggga ctatggaaga
     5221 aatacatgca aaaagttatt cttctatatt tatgactcta ctttctaatt tagaaataga
     5281 tgaattattt gaatggatag aaacagaacc aacattacaa agaaaggctg atttagtttt
     5341 agctcaatac gaaaatacta caaatcaaga gggattatac ctatcaatgg ttacaagtgt
     5401 atttttagag agtttcttgt tttattcagg attcttttat ccattgtatc tatctggtca
     5461 aggaaagatg gtagctagtg gagaaataat atcgcttatt cttagagatg agtctttaca
     5521 tggtaagtat ataggattgc tagcacaaga aatatacgat tcatttgata aaatggataa
     5581 gaagatgtta gaagagaaga tgtattctat actttatagc cttatggaga atgagataga
     5641 gtatacaaat gttatataca gagaaagtgg gcttgaaaaa gaggttgtga actttttaaa
     5701 atacaatgcg aatagagctt tagaaaatct agggtttgag aagctatata ctgtagatgc
     5761 tataaatcca attgtattaa atggtcttag cactgaaacc aaaacacatg atttcttttc
     5821 aactaagggt aatggatacc aaaaaggagt gtatgaggaa ttagaggatg aagattttat
     5881 aatataattt ataattatga cacactgttg tcacttttag cgtgataata tcaatgtata
     5941 aaatatatat ggaggtattt ttatgaaata tgaatggaga aaaagagata aagagttata
     6001 tttacctaaa aagaatcctg taataattga aataccagaa cagaaattta tcatgttaaa
     6061 agggagtgga gacccaaatt ctaaagaatt tattgaagca ataggagtac tttattcttt
     6121 ggcatatgca ataaaaacca tgcctaaaaa aggaattgta ccagaagggt attttgacta
     6181 tacggttttt cctttagaag gtatatggga taagggagaa aaaagtaaag gcttagatgt
     6241 acttattaaa gaagatttaa tttacactat aatgatacgt cagccagatt ttgttacaca
     6301 agacctgttg gataaagcta ctgatatagt aaagaagaag aaacctcata atcttcttga
     6361 aaaagttagg tttgggtcta ttaatgaagg gctatgtgtt caaatgcttc atataggttc
     6421 atatgataat gaaccagaaa gttttgacat tatgaacgaa ttttgcaaaa aaaataaact
     6481 tataagaaaa tcttacacac atcgtgaaat ttatataagt gatgctagaa aagtatcgcc
     6541 tgaatcattg aagacggttt tacgttttga agtagaaagg gaagtataaa tattttgaat
     6601 aatttataag agttataaaa agtgaagtct gttgagactt cattttttga tgcaatttta
     6661 aaattaaatt tgatgtttca taatatttta aaaactttct acttttatta gtgtgaataa
     6721 ctgttgctat taaataataa tactaattat ataagtatta ttatataata tttatagatt
     6781 attatataaa tagattatta gtaagatgta tgcattatta attaaaataa attacatata
     6841 ctatttaata tttcatttaa aatattaaaa aatgtagata aaaattgttt tattaaggaa
     6901 aaattaatat attgacaaaa tttaagtgct catttaaact attaattgat aataatattc
     6961 aatattaaat aataggaggc gttatgaata aatatcttct aataaatcca gtggcagaaa
     7021 gaatgtatgg agaaaagttt caccttatta aagcaaattt gataaaaaag gggtacataa
     7081 tagcagaatg tgaaccacag ttagaatatg taaaaaagca gtacaaagag tatacaaaaa
     7141 agactgaaaa tacaatgttg gattgtagat gtccagaaag tataagtctt ttaaaaagaa
     7201 ataatttgat aaataatttt gaagtaccta tgatagaacc aatattagta aggacttgta
     7261 gggttttata cgataaatat ataaaaagta aagatgatat gttggtagta acctgtccat
     7321 gtacacaatt aagagacttt gcaagtgaaa agtttaagga taagggtaat gctatgtttt
     7381 atacatggaa agagtttgct gaacaagaag gtgtaaaatc acttggaaaa atagatgaat
     7441 caccaattcc attaggatat ttcaaagata cttttgaaaa agttcttgaa gtatcaagtg
     7501 aagacgaaat attaagtgaa atacaaaaaa taagaaatgg ctttgaagat aagtatgaca
     7561 tagtagaaat gttttattgt aaagatggtt gtaataatgg agatggtttg taaatggtca
     7621 ataaatttaa taataagttt ttaaggctag taaagagtgt tctgatattt tttataattc
     7681 ttttattatg gaaaattaca aattatcttg gtatttggag tgattatata ttaccatcac
     7741 ccgaaaaggt atatagtaca tttttaaata tgataagtga tggttcaata tttattaatg
     7801 tatatgcaag tatgaaaaga gttttgatag gttttgcaat aaatactgct ataggtgtgc
     7861 ctttagggat attctttgga atttatagtg gagtatacga atactttaaa tctctaataa
     7921 attttttaag gaatacacct ccccttgctt taataccaat gcttatatta tggtttggta
     7981 taggagagga atcaaaaatt ataattatag ttttagcatc ctttttccca atctttacaa
     8041 gcacattgaa agggattaaa aattgtgact caaaactgat tgaagtgggt agagtatttg
     8101 agttttcaaa gcttcagata atatttaaaa taataatacc aaatgccatt cttgatattg
     8161 ctgttgggtt aaagttagct cttggatata gctttagagc aattattggg gcagaacttg
     8221 tagcagcttc atctggtctt ggatacttga tatctgatgg aaaagaaatg tctagaacag
     8281 atgttgttat tgtaggtata atcgtaattg gacttttagg aataattaca gattacatat
     8341 tttcgattat tgtgaagaag gtaagtaagg gaaagatggt ggaagcatat gaataattta
     8401 gtatgtgact ctggatatat aattagaaat ttatataaaa gttataaagt tgacaatgaa
     8461 gaacatgtag ttcttagtga tatctcttta aacatagata gaaaccatat aacagtaatt
     8521 ttaggtgaaa gtggttgtgg aaagactacc ttgcttagag caattgcatc acttgaaggt
     8581 ataaatagtg gtgagattag gtttattaat gataataaag agtatagacc taatgttggt
     8641 tttgtttttc aggagagcag attaatgcct tggttaaacg taagtgaaaa tatagctttt
     8701 cataatcaaa gaaaaaatac tattttaaac aaggtgctta gaaaatttaa acacaataaa
     8761 agaattattg gtaaaattat aaatgataat tttagaagct tagaaacaaa tgataatttg
     8821 aacaaatcaa ataaatataa taggaaaagt aatattgatg tagaaaaata tcttaagatg
     8881 atgaacttgc agaaatttaa aaactcttat ccaaatgaat tgtcaggagg tatggcacag
     8941 agagtaagca tagctagagc tctttctttc aatccagata tgttgcttat ggacgagcct
     9001 ttttcggcat tagattattt tacaaggatt gatatgcaaa atgaagttat aaaaatacat
     9061 aagtcaacta ataaaggcgt aatatttgta actcatgata ttgatgaagc gttaaagata
     9121 ggcaaaaaaa tagttgtttt cacagacgaa agaaaaataa aagaatttaa tatagaagat
     9181 ggttataata gagatttaac ttcagagtac tatattaaat taaaaaaaga tattttaatg
     9241 acaatgaaat caaataaaga catttaaaga aattaataat attttaagga gtagagtaaa
     9301 tgaaattaaa aaagctattg ataggtataa cgatattaac acttgcaaca gcatctttag
     9361 ctggatgtgc taaaaaaaat aatggagaaa aactttcaga aataaattta acttatgtaa
     9421 aatcaccatt aaatgttcct tctataattc agaaacagga tgatttattt ggaaaggaat
     9481 ttaaaaagga taatataaaa gttaactttc acgaaattac tacaggtcca gagcaaacac
     9541 aggctttagc tgctggagaa atagacttct tacatgcact aggtggtacg tcagctttaa
     9601 tagctgcatc taatggagta gagttaaaga tattaaacac ttatagcagg tcaccaaaag
     9661 gatttatgat tttgactaat aataattcaa taaaatcagc agcagattta gtaggtaaga
     9721 aggtggcagg tccaaaaggg actatattac atcaggtatt gatttcagca cttgataaag
     9781 aaggattaag tatggatgat gtggaatttg ttaatatggg tattccagaa gcatcagcag
     9841 ctctttctga tggtagcgta gatgttgcat tgattgctgg tccagcagct cttaaagcta
     9901 tgaagtcagg tagtaaactt gtagcaaatg gtgaaggttt agtagatggt ataatagtaa
     9961 ctgctgttag cacagacttt gcagagaaac acccagaaat agttgaaaga tttatgaagg
    10021 ttgaaaagga aactttagaa tatgtaaata ataattttga tgaagctatg gaaaaagtag
    10081 ctaaagaagt tgatttaagt cttgaagaaa ctaaagaatt atatgcttgg tatgatttta
    10141 gtttagatat aacagataaa gatatatctt cgcttgaaga tacacaagac ttcttaataa
    10201 aaaataagtt acaagagaaa aaagtgaata taaaagaatt aatctataat gctaagtaga
    10261 aactatgcta aaataatact aagtaagaaa tataataaaa gtttaagtta ccttattaag
    10321 gtaacttttt ctattttgaa ttagtaattt caatttattt tagaatgatt agttgataat
    10381 aatgattaat taatcataaa aagatacttt tttatagagt tttattaaga tgtctaataa
    10441 tatggttata cttatgaagt cagtaattgt gaagttaata atatgattaa atttatatat
    10501 tgtaaaaaga ttataatata ttaaaattgg actgttatat gatgagtaaa taggtatatt
    10561 aggaggaatt atatgagtta taaagtatat attgtggaag atgatatatc aataagtaca
    10621 ttacttaaag attatattga gaaatatgga tttgaagtga ttgttgaaga gaactttgat
    10681 gatataatga ttacatttga aagttttaag ccacacttag ttctattaga tgtaaattta
    10741 cctaaatttg atggtttcta ctggtgtaga tgtattagac aacagtcgaa tttacctata
    10801 atttttatat cagctagaga tggcaatgta gaccaaatta gagctttaga aagtggagca
    10861 gatgattata taacaaaacc tttttactat gatgtagtaa tggcaaaaat aaagagtcat
    10921 ataagaaggg tatatggtga atattcacct aaaatagacg aaagaatagt ggagctagat
    10981 ggattaaagt tttatccaga aaggttagaa ctagaattaa atggtgaaaa tctaattata
    11041 actaaaaaag aaggtatact gattgaatgt cttatcaaga gatacccaaa agtagtaagt
    11101 agagattatc ttttagaaaa gatatgggat gatattgagt ttgtagaaga aaatacttta
    11161 aatgtaaatg ttagcaggat aaggaaaaga tttgaggaat taggtataga taatgctgtt
    11221 cagactgtcc gagggttagg atataaatta aataagaatt ggtaaggaga aagtatgaag
    11281 ttatttttaa aagattataa aggttatatt tttatatatt atataggcat agttatgaca
    11341 attatttact gtaatcttat gaagtttatt agttttggag agagtttata tattctttta
    11401 tttagttctt ttatattgtt atgtttttta atatataagt attataaaaa tagagaagta
    11461 tataatctgt ttgaaaaagg aatacattct ttggaggagt catttttgta tttaggaagc
    11521 tcattcttgg ggagaaatat atcagttatt ttaaaacaac aacataaatt gtatcaatca
    11581 atgatacaag agcataaaaa gatacatagt gaacatttaa ctttcatcaa ccaatggatt
    11641 catcagatga aaacaccaat atcagttata agccttcagc ttcaagatta tgagggagaa
    11701 gaagtaactg aaaatataag aaaagaaata gatagattaa ataaagggtt agatatggct
    11761 atgcattttg caagggcaga taattttcag aaagacttta tagttgaaaa agccaaatta
    11821 aatgaaattg tatctagtat tgtaaatgaa gaaaaaaggt tatttataaa aagtggaatg
    11881 attcctaaga ttcaagtaga caaatttact tatgtttata cagatgtaaa atggatgaaa
    11941 tttataatag gtcaattaat tacaaatgga gtgaaatatt ctaaagatta tagtaactac
    12001 ctaactataa aatctgaaga aaataataaa tatgtaattt taagtataat agatgaagga
    12061 attggaattg tttctaaaga tttaaaaagg gtatttgacc cattttttac aggagaaaat
    12121 ggtcgtaagt ttggagaatc tacaggtatg ggtctttatc tagtaaaaaa ggtatgtgat
    12181 gatttaggtc ataaagtaag tataaaatca gaagtaggaa aaggtacaga agtttcaatc
    12241 tactttaata aagatgtaaa tagtttgata aagatgtaaa ttatctgtta caatagggtt
    12301 tttagttaat tgtacttttt tattatacaa tttattaaat gaaatattca tagttaaata
    12361 atcaaaattt aagagagttt atttgcaaac cttacataga tgtaaggttt gtgtaatgtt
    12421 tgcagatagt taaattttct aatctaaatt acaattaata taagaaataa catgagagaa
    12481 ataacataaa aaaagaaatt ttgtaaaaaa ggaaggatgt ttattgatga gtttagatgt
    12541 tatttttata ttgaaatcag tcataatagc tatagtagag gggtttacag aatttattcc
    12601 agtttcatct acaggtcata tgattttggt tggtaattta atagatttta aggggcaatt
    12661 tgctgaaatg tttgaggttg taatacaact tggggctata ttagcagtag tagtacttta
    12721 ttggaaaaag ataaaagata gcgttataga attttttaaa tttatattta ctggcggaaa
    12781 agaaggaaag attggtttta gatttggaat gaatgtaatt ataggttgta taccatttgc
    12841 gattatagga gttctatttt atgataatat aaaatcattg tttaatttac aatctgtaat
    12901 tataggattt atagtgggtg ggattttatt attagtagta gaaactttgt ttagaaaaaa
    12961 gaatcattca acagataata tagataaaat tactccaata caagctttaa aggttgggac
    13021 attacaagtt ttgtctgcat ggccaggtat gtcaaggagt gcatctacta ttatgggtgg
    13081 atggatagca ggacttaatt ctcctacagc agctgagttt tctttctttt tagcagtacc
    13141 agctatggtg gcttcttcag gaaaagattt atttgaattt gattattcaa taatgacacc
    13201 aacattgtgg attgctttgg tagttgggtt tatagtagct tttattgtat caataatagt
    13261 tatggaaaaa ttcgtgaatt ttttaaagaa aaaaccaatg agagtgtttg ctgtatacag
    13321 aataatcatg ggtgtagtac ttgcagtatt agcttttact aacataatta gtgtatagaa
    13381 aaaatagatt tattatctat aaaaattaaa ctaagataaa ttataattaa caattatata
    13441 tatgttattt caaaaattaa attaggaagg atagttaaat aatgaaaatt ttaactgtaa
    13501 ataatttaag taaagtatat ggtaaaaaaa taattttcaa cgcattaaat gacataaatt
    13561 ttagtataga agatggagaa tttgtaggaa ttatgggtcc atctggtagt ggaaaaacta
    13621 ctcttttaaa tatgatttct actattgaca agccaactac aggaactatg gaattaaagg
    13681 gaaagaatcc tttattgtta agaggtgagg aacttgcatt atttagaaga agagagttag
    13741 gttttgtatt tcaggatttt aatttattgg atacattaac tataggagaa aatatagtat
    13801 tgccattgac attagataag gtcagtgtaa aagagcaaga tgagagattg aatgaagtat
    13861 ctacaatatt aggtataaaa gatttattag gaaaaagaac ttttgaagta tcaggtggtc
    13921 aagctcagag gactgctatt gctagagcat tgattaacaa tccatcaata cttttagcag
    13981 atgaaccaac aggtaattta gattcaaagt catcaaaggt tgtaatggag ctatttcaaa
    14041 agataaataa agaaaataaa gttactacta tgatggtaac tcatgaccca ttagcagcga
    14101 gctactgtag tagaatctta ttcataaaag atgggtctat atataatgaa atatataagg
    14161 gtagtagtag agagcagttt tatcaagaga taatggatgt acttacatta ttgggaggcg
    14221 ataactaatg aatataaaac agtttgctat taacaatatt tcaagaaata ttaaagcgta
    14281 tttaggatac ttagcaagta ttgttatatc atcttcactg ctattttctt ttattatgtt
    14341 tacaagtcat ccagatttag atgtatctca atttcctgat tatttaaaag aaggtctcaa
    14401 gatgagtaag ataattgcat atctattctt attcttcttt gtgttttatt cagttagtgt
    14461 gtttcttaaa agtagatata aagaatttgg aatactttat attacaggta tatcaaaacg
    14521 ccaagtaatg aaattaatct ttatagaaaa tatgataata aatatagtat cttctgtaat
    14581 tggaataata ataggcttaa tctttgcaaa gatattttta gtggttatgt ctactttttt
    14641 agaattatcg ccattaaaat tctatatacc atttaatgcg atattgtcta ctttaatcta
    14701 ttttatgatt ttagctgtat taacatcaat atttacttat tttatagtta aagaaaatca
    14761 ggtgttgaag ttacttaaag gtacaaaaac tccaaaacca gagcctaaaa catcactaat
    14821 gcttgctata ttaagtattt gtttgtttat aatagcgtat tataatgcta ttacctcaac
    14881 cgaccaatat acaacatatc tgcgcttagt tccagttaca tcccttacta tagtagcaac
    14941 ttacttgttc ttttctcaat ttagtgtatt ttttatgaag aagctaaaac taaacaagag
    15001 attttataga aaaaatacaa atatgctttg gatttcgaac ttgatttata aggtaaagga
    15061 taatgctaga atatttttct tgataactat aacttctgca gtagcattta ctgctatagg
    15121 tacagtttat tccttttgga aagatgtaga gcgtcaaatt aatttaatat atcctaacac
    15181 aatatattat tcaacaatga cattgcataa tgaccctaaa aagcctgata gcaaagaaaa
    15241 agacaaggaa agaatgatgt ttatagaaaa taatctaaaa aaagagaatg tagactatac
    15301 aaggactaat ggtgaattta aaactgtatt tcccaagaaa agtgatttta atgtcagaat
    15361 tatgaaagaa tctaagtact tagaaattac agaaactata ggggttaagc ctataagttt
    15421 tgaagataat gaatgcatat cattattatc atcaagttta ccaggaaata ataacgtcaa
    15481 agaaaatatt attgtaggga atagaggttt aaaggtggca aaacaagtgg aagaatgtgt
    15541 tatgccagca tattacaaaa atatgtatgt ggtaaaggat aatttttatg atagtataga
    15601 atcaaaattt atagtggata gatttactgc atttgaagta gaggattcaa gtaaaactat
    15661 agagatatgc agagaatttg aaaataaatt taataatgag tcagggatgc aaccatattt
    15721 gtttttctct aaagaattaa tgataggaac tgggaaatta atatattcta catttatatt
    15781 tttagcaata tttataggtt taattttctt tgttactaca agtagttttt tatataataa
    15841 attttatatg gattgccaag ttgataagaa aaagtatgag caattaaata aattggggat
    15901 gacatataat gagattaaaa aagcttcaac aattgagatt ggaatagtat ttttgtttcc
    15961 ttatgtagtg gctgttatac attcaatatt tgcccttact gcactgaaaa attcttttga
    16021 tatagaagta gcatcatcag ctgttttggt aatgggaagc ttgttcttgg ttcaaatagt
    16081 ttatttttta attgtaagaa ataattattt aaaagagata aggttaaatt tagtcaattt
    16141 ttagttacta ttattaaatg ataataaaaa gggtttaaaa ttaattaata ttactggaat
    16201 tatagatgtt tattttttca gtaatatttt ttattgtatt aactattaaa attttgtaca
    16261 aatataaaat ataatatggt aaatgtcaaa attataaaaa aattctactt atataaaata
    16321 taaagaggtt atatgtacat atgtgtagaa ataaataata aaaatatcaa ttaatgttat
    16381 tagggggggt tactcattag taaattagct aatacactta gaatgatgat attaattcaa
    16441 agcagaggca aaatgaaatg caaagaatta tcagaagagt tggaagtaag tgaaaggcaa
    16501 ataaaaagtt acaagatgta tttagagcaa gctggtatat ttataaattc aacacctggg
    16561 atttatggtg gttatgaaat agataaatgt aatagcatat cattaattaa attattagat
    16621 agtgaagttt caattctaga tatgataaat tcacagttag agtataataa tgatatttac
    16681 aaaaatgaat ttaataatat tgtagaaaaa ataaaagcag tattaaatac aggagaaaaa
    16741 agcgatacat atatggatta ttttacagtt caagcacaac gtaattgtga ttataagtct
    16801 gaaaaaaata agtgtaatga aataattaga gcatatacca caaaacacaa attttggata
    16861 gaatattatt cattaaattc aggaaatagt gaaagaattg ttcatccata tggtttattt
    16921 aattataaat cagatactta tatggtagct ttttgtgaaa aaagatttaa gtttatagac
    16981 tttaaatta
//
LOCUS       DAGQWR010000090        16905 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.090, whole genome shotgun sequence.
ACCESSION   DAGQWR010000090 DAGQWR010000000
VERSION     DAGQWR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.090"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            323..781
                     /locus_tag="MIW95_002579"
     CDS             323..781
                     /locus_tag="MIW95_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY3151895.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFILGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLRTLKSL"
     gene            842..1117
                     /locus_tag="MIW95_002580"
     CDS             842..1117
                     /locus_tag="MIW95_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3151896.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1176..2450
                     /locus_tag="MIW95_002581"
     CDS             1176..2450
                     /locus_tag="MIW95_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBY3151897.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2580..3632
                     /locus_tag="MIW95_002582"
     CDS             2580..3632
                     /locus_tag="MIW95_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBY3151898.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     AVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDIDPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3632..4663
                     /locus_tag="MIW95_002583"
     CDS             3632..4663
                     /locus_tag="MIW95_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBY3151899.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKDIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4755..5567
                     /locus_tag="MIW95_002584"
     CDS             4755..5567
                     /locus_tag="MIW95_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBY3151900.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5570..6490
                     /locus_tag="MIW95_002585"
     CDS             5570..6490
                     /locus_tag="MIW95_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBY3151901.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6665..7111
                     /gene="rpiB"
                     /locus_tag="MIW95_002586"
     CDS             6665..7111
                     /gene="rpiB"
                     /locus_tag="MIW95_002586"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBY3151902.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7150..7815
                     /locus_tag="MIW95_002587"
     CDS             7150..7815
                     /locus_tag="MIW95_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBY3151903.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7925..9427
                     /locus_tag="MIW95_002588"
     CDS             7925..9427
                     /locus_tag="MIW95_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBY3151904.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTNIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNNSNLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9739..11331)
                     /locus_tag="MIW95_002589"
     CDS             complement(9739..11331)
                     /locus_tag="MIW95_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY3151905.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSYN
                     INCYYKEFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVK
                     FLENVISLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKYNNYNLKDMSITYVFFSNLLSMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFF
                     MYIYYSEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEV
                     IGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDM
                     LKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQ
                     SIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYID
                     MFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELEN
                     AHMEIIIDADLFKVMLTFRRVN"
     gene            complement(11300..12016)
                     /locus_tag="MIW95_002590"
     CDS             complement(11300..12016)
                     /locus_tag="MIW95_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3151906.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSREIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(12557..13357)
                     /locus_tag="MIW95_002591"
     CDS             complement(12557..13357)
                     /locus_tag="MIW95_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBY3151907.1"
                     /translation="MTFKNKKFTINKYEILSIILVLCLILTGFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNVGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(13672..14403)
                     /locus_tag="MIW95_002592"
     CDS             complement(13672..14403)
                     /locus_tag="MIW95_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151908.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(14390..15085)
                     /locus_tag="MIW95_002593"
     CDS             complement(14390..15085)
                     /locus_tag="MIW95_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY3151909.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(15087..15878)
                     /locus_tag="MIW95_002594"
     CDS             complement(15087..15878)
                     /locus_tag="MIW95_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151910.1"
                     /translation="MKNKFFTTLLTGFFAIVFFFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(15888..16700)
                     /gene="modA"
                     /locus_tag="MIW95_002595"
     CDS             complement(15888..16700)
                     /gene="modA"
                     /locus_tag="MIW95_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY3151911.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         6446 a         2076 c         2710 g         5673 t
ORIGIN      
        1 attcataata aataatttta taaaatagtt gttaaacata aaaaaccatt tgtaaaaaac
       61 aaaaaaatca tttctctaac aaaaaaaacc acaaaaaata taaaaaagta tggctagtta
      121 agtgattaac aagtaaaaat taaaataaaa atcaaaaaaa atttatcaat gaaagtcagc
      181 taaaatcctg gaagagagca ttattttcgg gaaaacattt tcttttgttt cacaaaaaaa
      241 gccacttttt ggcacgcaac atgctttata taaagtcaag aaaaactaag gtataagaca
      301 aaggcaatta ggaggtgtaa atatggagtt tataaatttt gtaagtgata aagttataga
      361 ccttaaaatg gattgtaaaa cgaaagaaga tttctttaaa gaaatacata ataaagtttt
      421 tgatttaggc tttgtaaaag aagaatttgg agaaaaaata ttagcaagag aaaatgtatt
      481 tccaacaggt ttaaatttag gtgattatgg agtagcaata ccacatacag atgcagaata
      541 tattaaagag caatttatat cagtctgtac ttttaatgaa ccagtagtat ttagttctat
      601 ggaagaccaa gatgaaaaag tacctgtaaa tttggctttc atattaggac ttaatcaacc
      661 acacagtcaa ttatctgtat taacagaatt aatgggaatt atgcaaaata aagagttagt
      721 tgataagttg atgagttcta ctgataaaga agaagtttta aggacactaa agtctttata
      781 aaaataaaaa agtactaaaa ataaaaaata taaaaataaa aattattagg aggaaataaa
      841 aatgaaaaaa atattagtag catgtggagc aggaatagca acatcaacag tagtttgtga
      901 caaggtagag agattagtta aggaaaataa tatacaagca gaagttatac aatgcaaaat
      961 agctgaatgt gcaacaaagc aagaaggagc agatttaata gtatcaacaa ctatactacc
     1021 aacaacttat aatataccag caataaaagc aacagcatat ataactggag taaatacagc
     1081 agcattagat aaaaaaatat tagatgcttt aaaataatcc tatcatagat acattgaaat
     1141 aatattttat aaaacagaaa aaggaggatt tataaatgga taaattatta gcagtagtac
     1201 aatacatttt aaatatggga ccgacagtaa tactaccaat aacaattgta gtactaggaa
     1261 tgatatttag aattaagttt acaaaagctt taaagtcagg tcttacaatt ggtataggat
     1321 ttgtgggtat aaacttagtt gtaaacctat taacatcaac actagggcca gcggctcaag
     1381 gaatggttga aagattcgga cttaatttaa cagtaataga tgttggttgg ccaacaacag
     1441 catcagcaac atgggcttca ccagttgcac caatattaat accagtttgt ttattagtaa
     1501 acttactatt attatacttc aataaaacaa agatattaaa cgttgatata tggaattatt
     1561 ggcactttat agtagcaggt gcagttggat atatagtaac aggaagttat gtttgggcta
     1621 tattctgtgc aatactaatg gagttattag tactgtttat tgcagaaaag acagcaaaaa
     1681 atgttcagga gttttatgat ttagaaggta tagcattacc aactggttca acagcttcat
     1741 ttgctccact tggatattta gtaggtaaat ttgttgaaaa aataccagga ataaataaaa
     1801 tacaagcaga cccagactct atacaaaaaa gatttggtat atttggagaa ccaatgatga
     1861 tggggattat actaggtata atacttggag cacttgcggg atatgatgta gcaggaatat
     1921 tcaatgtagg tatatcaatg ggtgcagtaa tgttacttat gcctagaatg gttagaatat
     1981 taatggaagg tttaattcca atttcagaat caataagaga tttcttacaa gcaaaatatg
     2041 gagacagaga actttatata ggtctggatg cagcagtagc aacgggacat ccagcagtta
     2101 tatcaacagc tcttatactt gttcctataa cgttgttctt agcagttata ctaccaggaa
     2161 acaaagtttt accatttgga gacttagcga caataccatt ttatatggca tttatagtgt
     2221 gctttagaaa aggtaatata gttcaatcag ttataacagg aacaatagta atagctatat
     2281 cactttatat ggctacaaac tttgcagatg ttcatacaca attattacaa caagcaaata
     2341 tgatgccaga aggaacaaca atggttacaa gtatagatac aggtggaaac ttgctaaaat
     2401 ggggaatatt gaaattatct caattagttt cttcatttgg aatattctaa tatgtagtta
     2461 atagagatta ctctttttta gtaaagtatg cgaaaattta acatttagca gctactaaaa
     2521 aagagtaata ttttttataa atttttaaaa atacaaaata caagaggagg agatttatcg
     2581 tgaaagcagc agtattacat ggcacaaatg atatgagatt tgaagatata gaaataaaac
     2641 catgtgaaag tgatgaagta aaaataaaag ttatggcagc aggaatatgt gggtcagatc
     2701 ctccaagagt attaaaacat tggaaatatc cagttccagc tataccagga catgagtttt
     2761 ctggagtaat tgctgaagtt ggtaaggatg taaaaaatgt aaaagttgga gatagagtag
     2821 ttgcaattcc ttttatacct tgtaatgaat gtgaatattg taaaagagga ttattttcat
     2881 tgtgtgatga ccatggaatg ttaggagcta aaagttttgg agcttttgca gagtatgtaa
     2941 atataaaagc tacaaatgta cttccaatag gagatatgga ctttgaagat gctgcaatga
     3001 tagagccact agcagttgct atgcatggag tacttaatat aggtgttcaa gttggagacg
     3061 ctgtagccgt aatgggtagt ggaacaatgg ggcagttggt aatacaagga cttaaaatag
     3121 caggagcagg aacaattata gcagttgata tatctgataa taaattaaga gaatcaaagg
     3181 aattaggggc tgatataata atcaatgcta aagatattga cccagtagaa aaaataaaag
     3241 aacttactgg tggaaaaggc gttgacatag ctcttgaatg tgcaggttca aaaataactc
     3301 aagaacaatg tttacttata actaaaaaga aaagtaagat aggattttta gggatagcat
     3361 attcagatat aactttatca gaagaagcat ttgaaaatat atttagaaaa gaactagaat
     3421 taaaaggttt ttggaactct tattctgcac catttccagg acaagaatgg actaagggaa
     3481 tcaatctagt taatgaagga aagataaaat taaaagaaat ggtatctcat agattctcac
     3541 ttgaagatac ttacaaagca tttgaaatga taagagatag aaaagaagaa tttaacaaaa
     3601 tattaatttt accacaaggg gttgaaaaat aatgaaagca gtagttaaaa ctaaaccagg
     3661 ctatgataat gtagaagttt tagaagtaga agaaccaaaa gctacaggag acaaggttaa
     3721 aataaaagta gaatatagtg gtatatgtgg ttcagatata cattcattta aaggtgaata
     3781 tgcaaatata aaagccccag taacattagg tcatgaattt tctggaattg ttgtagaagt
     3841 aggagaagat gttaaagata taaaagtagg agatagagtt acatcagaaa ctacttttgc
     3901 aacttgtgag aaatgtatat attgtgaaac taaagattat aacttatgcc caactagaaa
     3961 aggtattggt actcaagtaa atggaagttt tgctgagtat gtactatcaa gagaagaaag
     4021 catacatgta cttcctgaaa atgtatcttt attatcagca gcccttacag agccactagc
     4081 ttgttgtgtt catgcagctc ttgaaaagac tactatagaa agttctgata cagttttaat
     4141 aataggacca gggccaattg gtttattatt agctcaagtt gttaaatctc aaggtgctac
     4201 agttataatg tctggtgtta caaaagataa ggaaagatta gaaatagcta aaaatcttgg
     4261 agtagataga actgtaaatg taatggaaga aagtttggca gatgtagtga atgaaatgac
     4321 tgatggaaat ggagtaaaca aaggatttga ttgttctggg tttatgccag cagttaacga
     4381 agccttaaga ctgcttagaa aaaaaggtac tttcgtacaa gttggaatct ttgctaaaaa
     4441 attaaatgaa atggaccaag aagcaataat ccaaagagag ttacaatata taggaagtag
     4501 atctcaaaag ccaagttctt ggattacagc attagattta atggcaagtg ggaaagtaaa
     4561 tacagaagct ttagttacta gaactgttgg tttagacgaa tttaaagaag gtattacagc
     4621 tttaatgaac ggggaagaaa taaaagtagc agtaaaatct taaaaaaaca tttgtattat
     4681 tttaaaaaaa attgtaaaat ctactgtagg ataaaaaaat gtaaggtctt aaaaaataac
     4741 aggaggaaga aagtatgagc actcttagag atcattctaa taatattaga aaaaacataa
     4801 taaaaatggt tgcagaagct aaaagcggac atccaggtgg ttcattatca atagctgata
     4861 tgttgacagt attatatttt gataagatga acgtatgtgc agaaaatcca aaaatggctg
     4921 atagagatag atttgttcta tcaaaaggtc atgcagcacc agctttatat gctacattag
     4981 cagaaaaagg atttttccca gaagaagaat taataacact aagaaaattt ggttctaagt
     5041 tacaaggtca cccagacatg aaaaaagtag caggaatcga tatgtctact ggttctcttg
     5101 gtcaagggct atctgcagca aatggtatgg cacttgcagg aaaattagat aacaaagact
     5161 atacagtata tactatatta ggtgatggag aaatccaaga aggtcaaata tgggaagctg
     5221 caatgacagc atcacattac aaattagata atttaattgc atttgttgac ttaaatggat
     5281 tacaaataga tggttctaat gaagaagtaa tgaatgtaaa tccagtagat gacaaattca
     5341 aatcttttgg atggaatgta atagtaataa atggacattc ttttgaagaa ataggaaatg
     5401 cagttgatga agctaaaaaa gttacaggaa aaccaactgt aataatagct aaaacagtaa
     5461 aaggtaaagg tgtttcattt atggaaaata atgctgcatg gcatggaaca gctccaaatg
     5521 cagaacaagc agagcaagca ttgaaagatt tagaaggagg tctataggaa tgggaatagc
     5581 aactagagaa gcttacggac aagtgttaaa agaacttgca gaaaataaag atatagtagt
     5641 attagatgca gatttaggaa aagctacaaa aagtatatca tttaaagaag ttgccccaga
     5701 tagatttttt gacatgggga tagcagaagg tgacatgatt ggtacagcag caggtcttgc
     5761 tacttgtgga aaaatacctt ttgcgagtac ttttgcaata tttgcagcag gaagaggcta
     5821 tgagcaaata agaaactctg tagcatatcc aaatttaaat gtaaaaatag cagctactca
     5881 tgcaggtgta acagtaggtg aagatggtgg tagccatcaa gctatagagg atatatctct
     5941 aatgagaggt ataccaaata tggttgtatt aaatccagca gatgctttag aagcaagaca
     6001 agctattctt gcatctattg attataatgg acctgtttat ataagattag gtagagctgc
     6061 aactcctgat gtaaacagtg aaaattataa atttgaaata ggaaaaggaa ctgttttaag
     6121 agaaggttct gacataacag taatagctac aggaataatg gtagcaaaag ccttagaagc
     6181 agcagaagaa cttgctaaag aaggtataaa tgtagaagtt gttaatatat ctacaataaa
     6241 accattagat gaagcattaa taaaagaaac tgctaagaaa actggaaaag tagttacagc
     6301 agaggagcac agtataatag gtggtttagg ttcagctgta tgtgaagctt tagcagaaac
     6361 taaagatgta gtagttagaa gaattggtgt taaagatgta tttggtcaat caggtacacc
     6421 agctgattta ttaaaacact atggtttaac tacagaagat atagtaaaaa atataaaaga
     6481 gttgttatag atataaatat atctaaataa caaattgatt tgacataagt caattaaagc
     6541 tgtcttaaaa atagaaagaa atttctacaa tgggacagct ttatttttac aatactttat
     6601 ttttatgata attataaaat agtctgaaag taatcaaaaa aattataaaa agaggtgcta
     6661 tttaatgaaa atatctattg gatgtgacca tggtggttat gaattaaagg aatatataaa
     6721 ggaaaattta ataaataaag gtatagaagt tgaagatgtg ggtacaaatt caaaagaaag
     6781 tgtagatttt ccaatatatg caaaaaaggt aagtcaaaat gttcaaagta aaaaatctga
     6841 tttaggcata ctttgttgtg gaacaggaat aggcatgtct attgcagcaa ataagtttaa
     6901 aggaatcaga gcagctgttg tttctgactg tttttcagct aaggcaacta gagaacacaa
     6961 taatacaaat gtactttgtt taggagaaag agttattggg aaaggattag caatgagaat
     7021 tgttgaagaa tggttaaatt caagttataa tggagaaaga catgcaagaa gattacaaat
     7081 gattgatgat atagaaaaag aaaatttata aatatatata atatataatt tataacaagg
     7141 gggagcgtta tgtctattat ttgtgcttca ataatgtgtg cagaccaaat gaagttaaaa
     7201 gaggaattag aagctcttga agaagcaggt gtaaaattac ttcattgtga tgttatggat
     7261 ggaatatttg ttaaaaactt ggctatggga ccagagttat taaaatcaat aagtgaaaat
     7321 acaacaatac ctttagacat acacttagcc acagaaactc cagataaata tattgatatg
     7381 atgtcttata taaagccaaa atatatttct tttcatgtag aatcaagtac aaatgtaaaa
     7441 gctgatatac aaaagttaag aaattatggt atagggccag tgttagcaat aagccctcaa
     7501 acaagtgtgg ataaaataga agaatatata agtttagttg agggtatctt agtaatgaca
     7561 gtaaatccag ggtttgcagg tcaaaagttt aatctttctg tattggataa gttggataaa
     7621 cttacagaga tactaaaaga ttatgataat cctccattta tagaggttga tggaaatata
     7681 aataaggata ctattaaact tatgaatggt aaaaaagtag atatatatgt tgttggtact
     7741 tcagctttat ttaatgataa gcctccaatt tcatataaag ataaaataga agaactaaaa
     7801 gaaagtataa agtaaccatg taataaaatt ataatatgtt tctgtttaat agtaaaaatt
     7861 taggtggaaa caagaaattt tataatattt aataaaaatt ttggttgagt aaggagaatt
     7921 ttttatggga gacctatata aactacaaaa tggtactgat attagaggaa tagcgtatga
     7981 aaatgattct aaagaagtta acctaactgt agaagaagta aaaaaaatag ctaaagcgtt
     8041 tcatatttgg ctaaaagaaa agactaagaa agataaagta actgttgcca taggaactga
     8101 ttctaggata acaggaagcc aatttagaag tactgttatt gaaactttaa ctaatgatga
     8161 ttgtaatgta attgattgtg agattgctac aactccagct atgtttatga caactattat
     8221 ggatggatac aattctgatg gggcgattat gattacagca agtcacttac cttattacta
     8281 taatggttta aagtttttca cagaaagtgg aggtttagaa aaaactaata taaaagagat
     8341 gttggatata gctgttaaaa atgatagtgt agactatgag gaagtaaata aaaaaggtga
     8401 agttatagta aaaaatttaa tagaagatta ttctaattta ttgatagata aaattagaaa
     8461 aggtgtaaat tcaagtaaaa attatgaaaa gcctttttct gggcttaaga tattggttga
     8521 tgcaggaaat ggagctggtg gattctttgc tgaaaaagtt ttacacatac tgggagcaga
     8581 tacgacaggc agtcagtttt tgaacccaga tggtatgttc ccaaatcata tacctaatcc
     8641 cgaaaacaaa gaagcaatgg aaagcatatg taaagctgtg ttggataata atagcaacct
     8701 aggaataatc tttgatacag atgtagacag agctgctatt gtaggtaaaa atggcaaacc
     8761 aataaataaa aatgctctta tagctgttat atcatcaata gtattagaag aacatccaaa
     8821 aacagctata gtaacagatt cgattacttc tgaaggactt gctaaattta taaatgaatt
     8881 acaaggaaga catcatagat ttaaaagagg atataaaaat gtaataaatg aagctataag
     8941 attgaatagt gaaggtgaag agtgccattt agctatagaa acgtctggac atgctgcttt
     9001 aaaagaaaat tatttcttag atgatggggc atatttaata gctaaaattt taataaaagt
     9061 agcaaagcta tccttggaag gaaagactat agaagaattg atagaaaacc ttgaagaagc
     9121 tcaagaagag aaagaagtta gaataggcat taacaaagaa gattttagac catatgcaga
     9181 ggatatatta aaagatttag attctcatgt taagagtatt gatggatggt ctgtagcacc
     9241 aaataatttt gaaggaataa gagttaattg tgatgaatca aatggagatg gatggttttt
     9301 acttagaata tcactacatg aaccattact agctttaaat attgaatcaa ataaaattgg
     9361 tggagcagaa caaatttatt ctaagttaaa acttttctta gaaaaatatg atttaaaagg
     9421 tctttagggt aaatagaaag aatatattta tattaaagaa caaatgtaaa tttgtaccta
     9481 agattacaaa tattacaaaa ttgtaatctt aggtaacaga gatgtaactg attatggaaa
     9541 cactatgtga tattatagat acagagagat gaaatatgtg atgaattata gagaaatata
     9601 atttaaaatg tattaatatc acaccaaaaa ggtataatca aaaaatatgt aaatataaac
     9661 cccaacattt agaaaaagac ctctaaggag gtttttttct tgtttaaata acttaaaata
     9721 tatttttata ttaatatatc aattaactct tctaaaagta agcattacct taaataaatc
     9781 agcatctata attatttcca tgtgtgcatt ttctaattca attatatttt gagcaattga
     9841 taaaccaaga ccagagccat ctgtttttct agatatatca ccccttctaa atctttctat
     9901 taactcttct ggctttagat ttagtttatc atttgaaata tttttaaacg ttaatattat
     9961 attttcttcc tttataaaca tatctatata gaccctagtg ttaggcatag agtattttag
    10021 tatattgcaa attagattct caaaaactct ccaagttttt ttaccatcta tcatggtaaa
    10081 aacagcatat tcaggtgttt ctattataaa atctaaactg ctattttgaa ttttgtcttc
    10141 aaactctaca atactttgaa ttaaaagttc tcttatatcg gtttttgtta tattaaattc
    10201 tacatctcca ctacttagct tagatgcttc aaatatgtca tctattaata attttaatct
    10261 ttgtgatttt ttatctaaaa tatttatata gtcatttata gtctctctgt caaagtcatt
    10321 attttttaac atatcaacat agcttaatat agatgtaagt ggagttttta agtcatgaga
    10381 tacgtttgtg ataagctctg ccttcatcct ttcatttttt atattagcat ctacagcagt
    10441 ttcaagacca cctgttatat tatttatact tttggctaaa gtagatattt catcatctcc
    10501 tataacttct aatttatact ttaaattgcc attttctata ttttttatat tttttgatat
    10561 gttatttatg taattaaact taacagataa aaagttgtat atataaaaat gaaaaactgt
    10621 gtttacaagt gtaaataaaa ttactatagc tattgcctca gaataatata tatacataaa
    10681 gaatatcaaa acaagatatt ggataatagc ttgtacaact atagatatta aaaatactat
    10741 agctaatttt ttatttatag tttttttatt atacatacta agtaagtttg aaaagaatac
    10801 atatgttata gacatatctt ttagattata gttattatat ttaatatttt catatattat
    10861 atagactata gatataatta taaaattgta gattattaaa ttagtgttgt aaaacatttt
    10921 attacttgta aatgcatgaa accttaaggt taatagtatc aaagttaata taagtgtaag
    10981 ttctattttt accttagaaa aaaggcttat gacattctct aagaatttga ctttagtctg
    11041 tttatttttt ttatacaatg taaaacatag tatagtcaat aaaaaacttg ataatataat
    11101 acctatgcag acaaactcta attctacttt tgacaaatgt acaggagtac cgaaaaaata
    11161 aaaataaggg agagcaaact ctttataata acaattaata ttataagaaa agttattatt
    11221 caaatcataa actgcttcaa ataatataga aactaataga aacagtaata ataataaaat
    11281 aaattttgct ttagaatcat taataattaa gtttttcaat tttgtaacca atgccccaca
    11341 ccacctttaa atattttggt tcttttggat taatttcaat tttttctcta attcttctaa
    11401 tatgaacagc aatagttctc tcacaactat aagcatcttc attccagaca ttttcataaa
    11461 tttcacggga agagaaaatt ttaccttttc gtgacataag aagtgtaagt attttatatt
    11521 caagtggtgt cattttaatt tcctcaccat ctaaagaaac tatttttgta tctgtattta
    11581 gctctaggcc accacttttt agaatataat tagtatcatt tgagttattt gtattactaa
    11641 aatcacctaa atttatatac cttctaagtt gagattttac tcgagcagta agttctagtg
    11701 gattaaatgg tttagttata tagtcatcag cacccatatt aagacctata attttgtcca
    11761 tatcttctga ttttgcagat aagagaatta ttggtatatt ccttttttct cttattttaa
    11821 gtgtagtttt aagcccatct aaattgggca tcattacatc cattattatt aacttaatat
    11881 cattttcgat tagtatgtcc aatgcttcaa gtccatcata tgcctttaaa acattaaagt
    11941 tatcaggtct caaatagaac tctatagcat caactatctc tctatcatca tctacaacca
    12001 atatattttg attcaattta ttaaccccct tgaaatttta tttttaacca atttttattt
    12061 tataccaaaa agaggtatta gaaaacagta agcccgttca ttatgaaata aaaactagta
    12121 cttttaatat attgtcaatg catatagtaa atttaaaagt atagacgccc atattaatct
    12181 gaaggtaaaa gtttatgaaa atatctaggg taatctatca cactccttat gttattttgg
    12241 gtttattaat tttttaggtt taaacatata ttaacaaata taaataacaa atgagtttca
    12301 taattattaa gaatttataa caaatattta ttttaatgta gtttaatata aataggtagt
    12361 ttaatgtaga tatgtataaa gtatttaata aggcttatag gttttaatga tgatatcttt
    12421 atatatttta agagatgatg aaattagata tgggtaatag ttgaatgaga aaaagtagta
    12481 tagcacaaaa agtgctatat cacaaaatct caaataattt ccttcttata acttaaatta
    12541 aaagaataaa tatttcttat aaattaggtg tatgaagtat agcaggaaca tctttattat
    12601 ttaataactt atcaaatgat ttatggtcag acttatagcc aataccttct ctatattcta
    12661 attcatcaac aattttgtat tgttgacagg catttttaac cttttcatag tcttcagtag
    12721 aatgagcttt ataaactatc ttattattaa aagttatttt atcagcaaat ttaggtttta
    12781 caaaatattt agcatctttt attttaccaa cattatataa attttggata acagaaccat
    12841 tttttaaatg ttgtaaatgg tcatctaact tataagtatg tgctatatga gttacatttt
    12901 taaatttttc ttcaaatgta agagcaattt tttccatgta attgaagtca gtattaccat
    12961 ttgtatttgt ttctggtaat aatattatat tttcccttat tacaccaagg ctattaagag
    13021 aagctaaaaa ttctctattt ctatattctg atttttgtat atttgttagg ttttcatatt
    13081 ttttatattt atcaaagacg gatataccac atccagtaga tactaaaact acaaatacat
    13141 gattttttcc tctttctttt atagcattaa caatagcact cccagcccac aacacttcat
    13201 catcttgatg ctgaacatag aagacaacat aatctttaaa ttttttatga ctataattca
    13261 catagctact aaaaccggtt aaaatcaagc ataaaactag aataatagat agaatttcat
    13321 atttatttat agtaaatttt ttattcttaa atgtcattta atcatctcct gaaaagttgt
    13381 cttagttata ttatcaaaca aagtactaaa aattatttct taaatgctga aaaatataga
    13441 gtagattaat ggaataaaat tttttttatt agtttaaaat aaagaaaaca tttatgtata
    13501 agactaatca aaatattaat aattgatagg aattgagatt taaagggatt gtacatttat
    13561 tcgggattca ttttaataga attttacttt agtacttaga ataaaagtaa taacttttta
    13621 aatttaaaga gtacacatat atatttgtgt actccttgat gatatgattg cttatttact
    13681 acaaaaacag agtcttttta aacctaaaat atttgatgca ccagctatcg tagttccata
    13741 atagtaaact ggtttgttaa gtgatagaaa atttataatt gagttattac ataatgtact
    13801 tcctgtgcaa atcactaaat cagcccaaga tattacatct tcataatctc gtataccatc
    13861 ttcaatctta acattatatt gaatagtatc cacaaattca ggatttaaat ctaatactct
    13921 tgtttcgaaa aagtccttaa tattatcaat tatggctggt tgatatccta taattgctac
    13981 ttttggatta ccaaattcca tttttataaa ctcagggaat ttttttgcac atacctctgg
    14041 ctcgttattt ttacaatgaa tggttctatc cgtcttgcct aagtatttca taactgcatt
    14101 taatgatgct ataaaaagac ttctatcatg aggattgtca tgtaaatcta atgataatat
    14161 gtcttttaaa gaaccttcaa atgtagatgg agtagaagta aatgcttgtc ctttagctcc
    14221 tttaaagtca gcttcaagca ttatttcttt accaactata attgggaaat ctttacgctt
    14281 agtatttcca attgcttctt ttgaagatag agatttggac acaatactta tcatatccat
    14341 atttaaatta tatttatcga cttgtactaa tagataatct ctcaaaatat caaatatatt
    14401 catagaaaaa agattctcct ttatttttta tttcttcatt taaatcaaaa atatgattta
    14461 tttttccatt gttcattatt cctatcttat gagcaaaaaa cattgcttca gaaatatcat
    14521 gagtaacatg aatcactgtt ggtttgtatt ttatgtagac atccttcaaa attttgctta
    14581 tatgtttttt tgtaatataa tccaatgaac tcataggttc atctaaaagt aatatatcag
    14641 gttcagctat taaggcacgt gcaaaagcaa ctcgttgctt ttcgcctcca cttaaatttt
    14701 taggatacct attttttata tcttctatct gcattaattt agacaagtgg ttagccttaa
    14761 gaatctgttc atctataggt atttttctta atttaagtgg aaaccttata ttatcttcaa
    14821 cattcatatt tggaaataat tcaaaatttt gatatacaaa tcctatattt ctattttctg
    14881 gaggtaagtt tgatattaaa ttttctttgt atgatacatt tcctttagca gatggatatc
    14941 ttcctgctaa tgtttctagg aatagagttt tacctgaccc actagctcct aataaaacaa
    15001 agtattcatg attattaata tttaaactat tttcttgtaa tttaaaatta ggtaaatcaa
    15061 gagataaatt ttttattttt aacatattaa ttcttatcaa cctctctatt aaaaaatatc
    15121 tcaaaaagca tcaagcatgt aagtgagata aatatcaaaa ttacagctat tccgattgca
    15181 atgtctatat caccagtaga catatttaaa tatatagatg taggaataat ttcagttttc
    15241 atacgtgtag aacctgctat catagctact gcaccaaatt caccaagtgc tcttgaccaa
    15301 gtcattacag ttgcagatat gattccagtt tttagatttg gaagaattat aaatctaaat
    15361 gcttcccaac tattacatcc aagggtacgt gcaacaaata tcatcttttc attactttca
    15421 tttagtgaag tgtgaagtat tttaatagta agtggaagat ttacaaatac ttgtgctaaa
    15481 actatacctt gctttgtaaa tataaaatca agcttaaaaa tcttataaat ggaatctcca
    15541 atccccattc ttccaaacaa aagtaaaaga gcaattcctg atactaagtg tggaagagac
    15601 atagggagat ttattatctg tataatcagt tttttaaatg gtaatttagt aatatgtaaa
    15661 aaatatgcta ctggaatagc taataaaagg catactatag tagatattaa agatgtttta
    15721 atactaagtg ttattgcata ctgtacttcc acggatttta agcatatagg aacataactt
    15781 atccctttaa tgattaacat aagaagtggt gatacaataa aaaaaaagac tatagcaaaa
    15841 aatccagtaa gtagtgtagt aaaaaattta tttttcaagt ttaatcacta cttaacaggt
    15901 tcaaaaccat attttttaaa tatctgttta gcttcatctg actctatata atttataaat
    15961 ttattagcta aatctttttt atcactatag tttacgattc ctgctgatat gagttggtct
    16021 atatttttat cttgggcaat ttctataact tctacatctt cattgccaaa gaccgaatct
    16081 tttgtggcta ttgtagcatc agctttactt gatgttaact gtacaatcat ttcatttaca
    16141 gttgcagctg tagagacaac attatcattt atagatttta aattattttt ttcaagtatt
    16201 ttttgtgtag tcttacctat agcattagac tctttgtctc ccaaaataag tttaactcca
    16261 tctttattta agtcttccaa tttttgaata ttagctggat ttcctttagg tacaactatt
    16321 gcaggtgtat gatgtgcaac cgttttatgt tcacctacaa gttctttttc aaaggccttt
    16381 gaataaattg gttcagaacc aacaataaat acgtctcctt tgtgacttgt ctcaatttga
    16441 gctattagct gtgcagaacc tgcatagcta tattcaactt tgacattatt ttctttctca
    16501 aatttttttg ctatttcatc cattggtttc tttaaacctg cccctgaata gacaaaaaga
    16561 gtctcttttt cttttgaatc atcagctgtt gtcactttag tattagattc atcattagct
    16621 ttgttttctt tagaattaca tgcagtaata cttaatgaaa taatcatagc taaagctagt
    16681 actgaaagtt ttttaaacat aagatattcc cccttagtaa attagtgtaa attctttttt
    16741 agtatatcat actaagaaaa cgatgtctaa gtttttgtta tgttttataa tgttttgtta
    16801 tgttttatta tgtatagtta agaaaaaatc tatttataat gtcatttcca taggtagtta
    16861 ttttattttt aatttttatt aatactttaa agtattttaa attta
//
LOCUS       DAGQWR010000091        16712 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.091, whole genome shotgun sequence.
ACCESSION   DAGQWR010000091 DAGQWR010000000
VERSION     DAGQWR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.091"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(86..2224)
                     /gene="vanT"
                     /locus_tag="MIW95_002596"
     CDS             complement(86..2224)
                     /gene="vanT"
                     /locus_tag="MIW95_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBY3151912.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(2242..3048)
                     /locus_tag="MIW95_002597"
     CDS             complement(2242..3048)
                     /locus_tag="MIW95_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBY3151913.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(3045..4145)
                     /gene="vanG"
                     /locus_tag="MIW95_002598"
     CDS             complement(3045..4145)
                     /gene="vanG"
                     /locus_tag="MIW95_002598"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBY3151914.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(4406..5488)
                     /locus_tag="MIW95_002599"
     CDS             complement(4406..5488)
                     /locus_tag="MIW95_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY3151915.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(5538..6239)
                     /gene="vanR"
                     /locus_tag="MIW95_002600"
     CDS             complement(5538..6239)
                     /gene="vanR"
                     /locus_tag="MIW95_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBY3151916.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(6673..9204)
                     /locus_tag="MIW95_002601"
     CDS             complement(6673..9204)
                     /locus_tag="MIW95_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBY3151917.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVKEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(9312..9929)
                     /locus_tag="MIW95_002602"
     CDS             complement(9312..9929)
                     /locus_tag="MIW95_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBY3151918.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(10291..11175)
                     /locus_tag="MIW95_002603"
     CDS             complement(10291..11175)
                     /locus_tag="MIW95_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBY3151919.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAVKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(11366..12616)
                     /locus_tag="MIW95_002604"
     CDS             complement(11366..12616)
                     /locus_tag="MIW95_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3151920.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFIYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDCKAI"
     gene            complement(12838..13602)
                     /locus_tag="MIW95_002605"
     CDS             complement(12838..13602)
                     /locus_tag="MIW95_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3151921.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(13596..14321)
                     /locus_tag="MIW95_002606"
     CDS             complement(13596..14321)
                     /locus_tag="MIW95_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151922.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(14323..14691)
                     /locus_tag="MIW95_002607"
     CDS             complement(14323..14691)
                     /locus_tag="MIW95_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY3151923.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(14867..16411)
                     /locus_tag="MIW95_002608"
     CDS             complement(14867..16411)
                     /locus_tag="MIW95_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBY3151924.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQFISKYAIQIILIMAIICMMLIFYIIYDKLNFKIKLQKIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIDLNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         5737 a         2953 c         1664 g         6358 t
ORIGIN      
        1 tatatattag acataatatt actaaaaatg tctagtcaga gtaaaataaa ttacacagta
       61 caacaaatat tactatttaa ttgagttact tctttataac atttaacctt cttcctactc
      121 tgctcaataa ttcattcgat atactacatg agttatttgc aacttcagaa gcagataatt
      181 cagataaatt atctcttcct attattattg ccgtattacc aacttctaca ttaggtatat
      241 ctgtaatatc aacagcaagt tggtccatgc aaattcgacc cactattgct gctctacaac
      301 catttattat tacgtaactt tttccacagg ataaatttct aggtatacca tcggcatagc
      361 caatagataa tatagcaatt acgctatctc tgcttgcaat aaagactctt ccatatccaa
      421 aactatcatc tttttgtatt tttctaataa gaattacttg tgattttaag gataaaacag
      481 gtcgtaaatc aagttgcaat ttagtcgtat catttggaga acttaatatc ccgtataatg
      541 caattccaat tcttacataa ttacacttaa gattgggata attaaaaaaa ccataactac
      601 tttgaatatg tactttagga atttttatac ctttttttaa cagaaaatca attaacttat
      661 aaaaacattc aatttgttta tttgtaaagt ctacatcttc atctttcaga ctatctgaaa
      721 cacacaagtg tgtaaatatg ccacatatat ctaagtattt taatttaaat gcctttaaaa
      781 ttgacctcac atcattaaca tcaaaaccca gtctgtgcat acctgtatca attttaatgt
      841 gtgattttat gttgtatttc tgtttgttta aagagatagc atgattaagg tcaataacac
      901 tctgtgtaag attgtattta tgaagttcct ttgcccttat tgggttagta tatccaagaa
      961 tcaatatttc tcctagtatt ccatatcgac gtaaatcaat cccctcatct attgttgcta
     1021 ctgcaaatgc ttttacacct atttggtttg cacaagtcgc tacttcaaaa gcactatgac
     1081 cataggcatt agcttttaca actgccatca attcacaatc ttctggcata gctttttgta
     1141 tcatctttac attatgttcc aaattattta aatcaatttc aatccaagct ctattggtat
     1201 ttaccttatg ttctcctttc aagtcttttt ttagaggttt aagaatcaat ataataatta
     1261 tagaagctat aaaagatgct atacttacca ctatataatg aataaggcta ttgtcaacca
     1321 agacagtttg tattccgaat attttagaaa acatacgaat aacaacaatc atcataggat
     1381 gtataatata aataattaat gatgatgttc gcaataacct agctcgagaa ccttttaaaa
     1441 atgttaaggc tgtaaataag aaatacatac atggcaataa cataatatac atgctatcat
     1501 gacgctgtac ccctagttta tgtaaaaaca ttccctcaca taacataagt aagaatgata
     1561 taagaaatcc aatcaagcta tttttagttg acatataatt agatttatta gatattattc
     1621 caccaagtat gaaaaataca ggtgcaaaaa atataccatt tcgtgtataa tctgatactt
     1681 caaaaatata cccatataaa ttctttaata gtggtatttt ttctaaaaaa ccataataac
     1741 tatcaccaaa cataccaatt acataaataa caatagtgat tactaatgag tactttaaac
     1801 ctagtttttt tacaagaaag tatgcaatac ttgcaccaat aattgatgca ggtagatacc
     1861 ataaatgata taaagtccca tcaaatacaa tgtccttaat aatattgggc actaaattat
     1921 tcatggaaaa atatccagtg taaatattta agggaatata aattacaata gctatcccgt
     1981 aaataaataa tacatttttt agaaaagatt tcagtttatt acaattataa ttatatgttg
     2041 aaattagaaa aaagcctgat agcataaaaa agaatggcac agcaatacga gcaattatac
     2101 tagttaacac aaagtcagcc attttactat atgataataa tggagatgta tgaattgcaa
     2161 ttataagtaa tgcagctata atcctaaaat aatcaagccc tgcataagat ttattcctaa
     2221 tcattcttgt tcctccattt cttagtcctt tctatttctc caaaatgtca caataaaacc
     2281 atccttattg ttacccgata tttgatatac acattgttca ggtatagaga ttctcacttc
     2341 ttctttagta tgaataggca cataaaagat ttctataatt ttttctttat cttctatttt
     2401 taaacaattt gtatacgaaa atctcttaat atattctata tattcttcaa gggcgagatt
     2461 cattttattg ataatctcag aatgtggata tccgacataa cgaaaatgcc atggttcttg
     2521 agatattcct gttatttctt cttttttaca ctgataacgc tctataaaac cataatgtag
     2581 tgctgcctta cggaactcat tacaaatacc attataagga aattcagggc agataaaatc
     2641 tataacctct ttcttcaacc ctaaatcaat agcaagccct gtctgatgtt cactgtgatt
     2701 tggaagtgct acaaactttt tagtaaagtc ttttccattg tcacgcagag agcttgaata
     2761 tatatcttgt tgttcctctg cagtacgata tccacttaca ggtactattt catttattcc
     2821 acatatactt tttaaaatat tttgtaaaat atttgtagct tcacgtttca taagaatact
     2881 tggaaacctt gtatctacag gaattaaatc tatatcttca gatgttttaa ctggtaaaaa
     2941 agcatttact agaattagat tcccatcata aactttatct ttagtcaata agatagtatt
     3001 tgtcatttta tcttctttat ttttatctaa taaaatatta ttcatcattt tatgtacacc
     3061 tcaattatct tatccaatat ttcttcaaaa gacagtccaa tacctttcat catattcgga
     3121 taacgactat gagaagtaaa gccaggaatt gtattgactt cattaaaaat aatttcacca
     3181 gatggagtta aaaacatatc tactctagca aatccagaac atccaagagt tctataaata
     3241 atttttgctg tctcttgtat tcttttctca caatccaaat caattctagc tggcatatga
     3301 atttttgaac ttttcaatgt atatttttct gtataatcaa aaaatccttt tgacaattct
     3361 atttcatcta ctcttccaac aagtaagtca tcgtttccta aaactgcaca ccctacctca
     3421 aaaccatcaa tattttcctc aattattact tcattatcat gttcaaaagc caagtctact
     3481 gctacttgta actcttcttt tttataaatt ttactaatac caaatgatga acctgcacgc
     3541 acaggtttta taaataatgg ataagttaag tcttttgaca gttcagccac attaaattta
     3601 tctgagtatt ttagtaatat tgattttggt actttaatac cttctgaatg aacaagttta
     3661 tgtgatttgt ctttgtccat acataatgct gaagaaagag taccacatcc aataacctgt
     3721 atacctgcca attcaaataa cccctgcact gttccatctt ctccattttt tccatgcaat
     3781 acaggaaata caaaatcaag tttagtgtat ttaatatcat tttcacatga ttcaatagca
     3841 ctatttggaa tatgattctg agaaacagca tcaggaatta aaaattcaat aataccattt
     3901 ttgacacggt tctgcgaaac aataactgga attatatcag tatccctttc aaaccaagtg
     3961 ttattctgga tattattata atctccaata taatgatacc aattaccctc ccttgtaatt
     4021 ccaattggta taatatcata tttttccgta ttcaaattct caagtactgc aaaagctgat
     4081 tgtaatgata catcatattc tgttgagttt ccaccaaata taactgcaat ttttttatta
     4141 ttcatctttg attctccttt gttgatttat ttatttttat acaatctaca gaaacatgtt
     4201 aaaatacttt ttctatttca aatccaatca accttcactt acaattgcat ctgttccaca
     4261 taatatattt tcacattttt ctattgatat aattactgta tcaaaataaa agcactcttg
     4321 ttttgataca gtaattatat caataacaag agtgctcttt atttatttat cctaaagaat
     4381 ttcctatttt tttatactta aattcttaca aatttcctac aatcggaata ataaccttaa
     4441 aggtaattat attgttctcg ctatgagctg ttattgtccc gttatgaagc tccactattt
     4501 ctttagctat agcaagtccc aacccagctc taccactgtt tgaattacga gatgtatcca
     4561 acctgtaaaa ttgttcaaaa atacgctcaa gtttttcctc tggaatcgta ttgctatgat
     4621 ttataaattt aagctttata taatcatcct cctgtacagc tgttatttca atagtagtat
     4681 cttcaaaact ataataaaca gcatttcgta ataagttatc aaatacacgt tgcattttat
     4741 taacatcaca ccttatcatc atatttggtg taacattcag tgtatatttt aaattcttac
     4801 tcatcaaagt aggtttaaac tcatatgtta gttgttcaag catacgagtt aagcttactg
     4861 tactgtattc aagtgatata tttgataaat taaaccttgt aatttcaaag aattcattaa
     4921 ttaaatcttc aagacgctct gccttatcca acgaaataga taaatatttt tttcgcagtt
     4981 cttcagaaat ctgactttca tctcgaagta gtgtcagata ccctataact gatgtaagtg
     5041 gtgtttttaa atcatgtgca agataaacaa taagttcatt cttacgttgt tctgccattt
     5101 gtgtctcaaa tttacgttgt tcaagtatat gtttaattga gtttattttc ttttctattg
     5161 ccaataactc tggagataaa gccacttctc caacatcttc tttgataaga gagtcaatac
     5221 cttgatttat ttcactaaaa tattttgtaa accaatttaa ataaacacgg aatattataa
     5281 aaaacacaat caccatagct agtatcatga agagctccat gctattacgg aaaatctgct
     5341 gatataaatt caatgcagct ttatattcca tacgaaaagc attttcaaag aatgatacta
     5401 tccagataga aaacttacca tacaatataa aagaatataa aatccatatg aaaataatgg
     5461 ctataaatgc cattaaagct gttctcataa aaattttatt tctcacttga gtaaagtcag
     5521 gattgacttt aatattttta cttttcaatt gtataaccta ctccccatac agtttttata
     5581 aatttcgggt ttttagaagg ttcatgaatt ttttcacgta atcgccctat atgagccata
     5641 actgtgttat tattatcaag atatttttct ccccatacag cttcaaacaa ttcttcagaa
     5701 ggaaccactt ttccttgatg ttcacataaa taccaaagaa tagaaaattc aatcggtgtt
     5761 aatgctactt ctttcccaaa taaactacat ttatgtgttt ctttattgat aatcaatcct
     5821 ttaatatcat actcattaac aattatcgac tgttgttcat aagaattatt atatcgcata
     5881 tatcttctta attgtgtctt tactcttgca acaacttcca aagggttaaa tggctttgtt
     5941 atataatcat cagcacctat ggttaaaccc attatcttgt ctatatcttc aattttagct
     6001 gttaacataa tgataggata atagtatttt tctcgaattt tctggcaaat atgaaatcca
     6061 tcaatatcag gaagcataat atctaaaata gccatatcca aatctttaga ttcaatacat
     6121 tctagagctt ccttacctgt ataaaacttg tatactgtaa aaccttcatt ttttaaatat
     6181 acttcaatta aatctgctat ttctttttca tcatccacaa tcaaaatatt ttcgttcata
     6241 gattattttt cactctcctt attatcgagg tcatcattta taatattggt aaatttacat
     6301 atcaaataat tacaagcata tttgtaacta atttttataa gaaaagaact actacatcag
     6361 aatgtgttga tgctactatt ggaacacaaa aactgagatt aattgttatt ataaaaactc
     6421 ccctaacacg tccttattat cgcataacag gtattaactt tcaataaaaa tatctatcag
     6481 tcaaacacaa ttcaatttat tataccagag acaatccata taaaaaacaa ttaaacctat
     6541 gtatgtaacc acagacatta ctaaaatttc catgttattt tatataaaaa ttcaaaaaag
     6601 gagttgaatt taaaatttga ttagaaaaaa atcaattttg agacaactcc tttttatttt
     6661 aatgtattta aattatagta tatcagcctt cataatatcc tcaaaatcac ctttttcaga
     6721 tattaatctt atcaatttaa cagtacagct taaatcattt ataagtatag ctccaactaa
     6781 tgaaccatct ttcataaata attttttata tattctttga ccttcatctt ttgatgaaat
     6841 ttctttatca aaattttgta tattaccaat actaaatact tttacattca ttgcatctaa
     6901 tgaaactgga tatacttcac tttcaaatgt caaattatct ccacttgcat ttgctcctgc
     6961 tatttttccc atttctactg aagatggcca tattgctaaa ctaatatttc ccacttgtgc
     7021 aacatctcca caagcatata tatctttaac atttgtctgt aatgtcttat ctacaacaat
     7081 acctctatca atttttacag atgtatcctg aaccatttga gtgtttgctc ttacaccaat
     7141 tgaaaatact accatatcac agtcaacact atctccatct ttaaatacaa ctttttgagc
     7201 ttttccatca ccttcaatac catcaactgc tacacccaat cttacatcta ttcctgtatc
     7261 tcttacacat tgttctaata ttttactacc ctctttatca agttgttttg atagtatgct
     7321 atccatggct tcaactacta ctacttctaa tccttttagt ctaaattccc atgcagcttc
     7381 aagtcccaat aaaccgccac caatcactac aactttctta gatttttcta gtgcttcttt
     7441 tacactatat aaatctttta tatttctaag agtaaacaca ttttctaaat catggccctt
     7501 aatcggtggt ataaagtttc tacttcctgt agctattaca agcttatcaa atttaatagc
     7561 tccatcattc actataatag ttttattcac ttcatctaat ttatctacat tcgttcctaa
     7621 tattactact atattatttt tatcatacca atctttatct tccatgtaga aatctgaacc
     7681 aagttcttca tttataccat cagaaagtgc aggtctatag taagtcaact catcttcatt
     7741 agaaatcata gttatcttac aagttttatt tctttttctt atagcatctg ctgcataaaa
     7801 accagctgct ccatttccta ctattacgaa atattcatca gtatcttttc taaaagtaac
     7861 ttcatcttct tcaacttcta taaattggtc ttccttagct ccacatacag ggcatacact
     7921 aggagcacta tcaccttcaa aaacttcatt acaaattaca catttaaatt tcttagtttt
     7981 ctttttactt ccaaaagtag aatttaactt atctaggaat ttacctgtat aagaatgtaa
     8041 tttatcaatc tcttcatttg atggtttaaa gttaacagca aaaggtttta ctgctgtcat
     8101 tctaagctga cttattctct ccatagcatt ttctatacct tctccactcc atccatatga
     8161 accaaatact gaagcaactt ttccaccatg aagaactggg tttaacgata ttaatatatc
     8221 ccatataggc tttaacgcat ctcctaatat tgttggtgtt ccaaataata caccttgtga
     8281 tactgctatt tttgccaaaa tatcttcttg cttatgctct attacatcaa ataaatttac
     8341 tttatagttg ctattttttt caatatatgc ttttatagct tctgccataa tctttgtata
     8401 tccatgtgca gaaacataac aaattgtaac ttctttttct aactttattt gttcctttac
     8461 actccagtta taataaaggt ctataatttt tctaggattt tcagttaata ctggaccatg
     8521 acctggacat acagtatcta tatctaaatc ttttatcttt tctatagcag ttaccataga
     8581 tggtttaaat ggacccatta tacaatcata atagtatctt aaagcatcca agtaatcctt
     8641 ttcttcattt tcatcaagtg tatttactat cttatcatta ctatagtgac taccaaatga
     8701 atcacatgtt acaagagttt tatcctcttt tatatatgtg tatatagtat ctggccaatg
     8761 aagcataggt acagagaaaa attctaaagt tttgtcccca attgataatg tatctccatc
     8821 tgtaactgca acatattcaa agtctttgtt tacgatttcc ttaagatatt taattgctgt
     8881 ttctgaagct actacttttg catttttagc taaatccaaa agcttttcaa cgcttcctgc
     8941 atggtctggt tcagtatgac ttacaacaat atagtctatt ttttcaaaat ctatatttaa
     9001 atcatttaat ctttcaatat aattgtcaaa atgcttatct ttaactgtct caaacaaaac
     9061 tgttttttct gttcctttta atacatatga attataagta gttccataag gtgtatacat
     9121 aattatgtca aatactctta aatcagggtc aagtgaacct acccaatata aattttcttt
     9181 tatttttagt gatttcaatg acataccgta ctcctcctaa aatagttatt tagtctcttt
     9241 ataccctgct tgttatattt taaacaaaat ttattcaaaa aagaaattat tttaaagata
     9301 gtttaaatct tctaatcttc tttttctacc ttcaatacag ttcccttttt agaatctact
     9361 tttatgtcat attcattttt atctttatct attcttattt tataaaaagt aacatctaaa
     9421 tctttgtcta aactccattc tttaaccatt ccattttgtt cttttaatgc aatttccata
     9481 gcttgttgag gtgtaattat gtcatttaaa tctaattttt cttttctagc aactccattt
     9541 aaatcttctg aatctaactt ttctgtttta tcttctaata catcttttgt attagcatca
     9601 attttcatct tatattcatt attatcatct acaccttcca cagtataaac aaatttattt
     9661 aaatctctct caagacttaa atctactata tcagcatcct tgtatttatc tttaaatatg
     9721 tttattgcat ctgtgtatgg cactttaaaa tcttgttcca ttaaattttc attatttttt
     9781 tcatctttta aagcattatt tgaagcattt gcctcctttt ttgtattatc ttgtgcttgt
     9841 cctgaatcct tagttgggct cccactacat gcagttaata aacctgctat aagtatactt
     9901 accactaata aatacgtttt tttacacata aaaatcctcc ttaaatatat taatttattc
     9961 tatattatgc cctctaaacc ctttatttac ccttttatat taaaaattcc tcctatttta
    10021 tcaattttaa aactttatat ttactaaaat taaagttaca taaaaattat actgtctcaa
    10081 aatagaaaca agcatctttt tcattgttga aataataaaa atatatttta tttcgtacaa
    10141 aaagagtgcc tcatgaacta aaaagttcat tttgagacac tcccattctt ttatattttt
    10201 acttacaaaa taacagtact tattaatttt ttacaaacct tataagtata tctatattat
    10261 attcttaaat ttaaacacta tattaaaaaa ttaaattttg tttaattctt ttactgttaa
    10321 atccatttta ggatatggaa tcgtaattcc ttcttcgtca aacctaactt taacctcttc
    10381 tagtaaatca agataaattg tccaataatc ttctgtcttg caccaaactc tagttgcaaa
    10441 tttaacagca ttatctccat gttctactac tcctataaat ggttctggct catttattat
    10501 taatttatga ttattaacaa tatctttaag tactcttcta acatgtagta tatcatcttc
    10561 ataccctaca ctaaaagtca aatcaactcg tctagtattc tttgctgaat agtttattag
    10621 actatcattt gatacagaac cattaggtat aagtatctgt ttattatctg gagtaaccaa
    10681 ttgtgtataa aaaagtccta tttgttcaac cgttcctcca tggcctgctg cttctacgta
    10741 atctcctact ttaaaaggtc taatcaatag tataataaat cctccagcaa aatttgatag
    10801 actaccttgt aaagccaaac ctattgctac ccctgctgat gctatcacag cagcaaaact
    10861 ggacaaactc attccccaat attccagaac aatgattata acggcaactt taagtacact
    10921 caaaagaaga gacttcaaaa accttcttaa agttaaatca atatctcttt tactaagtac
    10981 taacataaca tggtttacaa atttttttat tattttaaat ccaatagaaa gtataagtat
    11041 acctattata agttttactc cgcttgtagt agcccattct agcagacttt ccattatacc
    11101 tcccatatcc atcttattaa tgtctaaatt atttaaatct aacttgctta tatcatctat
    11161 tttcgtcgta tccataaaaa actccttacc ttatatttta gaaccaatat tatagtttaa
    11221 agttcttatt ttctatttaa tataatacaa taccatttgt acatagtaaa caagatttat
    11281 attccaatta tacatcttag gaaaatgcaa actatttgtt atatatatgc aaaatataat
    11341 actaaattaa actattaata aaacactata tagccttaca atcatcattt tttaccccta
    11401 tatgagatga tactatagtt ataatactta gtactataaa tactacaaaa ccttttttat
    11461 atccatcaac tatattatat gaatttactt gtaaagagat agctataatc aaattaataa
    11521 ttccacttcc tataaattca aaagtattaa taacactagt tgccagtcca gaataaattt
    11581 ctttattttt atacttggca tcattaaaaa cctgtagatg tgacataact atacatccca
    11641 taataaagaa tgatattggt aaaactataa ttggtggttt tacctggtat acaactacaa
    11701 ttacaatcca tataaacaaa tctatcattg ctccaaattt tattatatta aatttgctag
    11761 accttatttt agcaaataca aaatccataa ttattgaacc aaatataaat ccatatgtaa
    11821 aaaatgatac tataaacgca gaaaaactct tactcacacc ataaacgtcc attatgtatc
    11881 ttactcccca caagcttata aatgcacttg taagaccaac aaaagagaac attatcattg
    11941 aattatacca tgttgatttg ttctttataa cagacttaat accatcaaca atattaacct
    12001 tctctgactt ttcatagggt tcagtatcta cattgaatcc atactctttt ggtgtatctc
    12061 taacaataat atatataaaa catccaatta caacaccaat tataccgatt aataaaaatg
    12121 aatttctcca accaacaagt tcagacaaaa acacaagtgg aaaagtagca agaactccac
    12181 ctaaatttcc tacaaatgaa aatttagctg ttgcagaagc aaattctgac ttattaaacc
    12241 atctcccttg aatttttaaa atacaaagta atattactga tgttcctgcc cctacaatta
    12301 cacgagaaat atatgctaat tctatacttt gaatcaaacc aaataaaatt gaccctaaac
    12361 ttgccattat tattccaaga ctgctaattt ttcttgctcc atatttatca atcaaaattc
    12421 ctgcaggtat ttgcataaat gcatatgcat ataggcaaaa ggatgctata tttgaaattt
    12481 gtatagaatt aaaacctaac tcatttgcta aattgtctga aacaacagca gttgacatcc
    12541 ttaaaaataa tactaaaaca aatgaaaatg taattatacc ccaaacactc cacttagcct
    12601 ttgtataact tttcatatct tctccctcaa atccatatat ttttaaattt tcttgatata
    12661 acttatataa agtgtatagt tattcttaca aatttttaca cgcaatatca ttatacaaca
    12721 tatgtatata tttaactaga aataaaatac tattcatatt atatacatga attaactaca
    12781 taaataataa gagccatctt ttaacattac tcaaaagatg gctccatata aaaacattta
    12841 ttctaaattt aattttttat ctaatataaa ttttgtagcg aaaaataacg ctattgcaac
    12901 tacaagacta ccaagtattg caagcataaa actaggactc tgataaagat gatataccat
    12961 atttgtatct tcatttggta agatgcttcc aactgcatct ccaacaattg atattactat
    13021 atttatagct ataaaagaag caaatgccac tatgtttcta tgtttattga attttggtaa
    13081 ttgtcccatt gagattgatg tatataataa caaaataaat gatgaatatg aaataaacat
    13141 tactactact aataataaaa tatctaaaaa gttttcagaa atccatttaa aactagtgtt
    13201 aaacaaatct aaaatttcag gtagtaaaat tccagaagta ccaaaaagca taagaactcc
    13261 aaaagtgaat actgcaacta taaaactaag tacagcgtat ataagtgctg ttatacattt
    13321 agataatata agtgaactag tgctaacagg aagagtaaac attaaataac cttcatcccc
    13381 caaaagattt tttctaaacc tttgaattgt aactacaata gttaaaactc ctaatgccat
    13441 aaaaagactt gttaatacta gtattcctat tccctgtacc tgaagtatat ttgtattcat
    13501 aaatattcca ttaaatacag ctactatcaa tatggctata tataacggta taaatattct
    13561 tccacttgct tttaactcat attttaataa tttacctaac atttaaattc ctccctaaat
    13621 aatgcatcta tagattttcc tttttcttct cttattgctt caacgtctcc ttgtaacact
    13681 atttctcctt ttgatatgaa tattatctca tcacatatgt tttctatttc acttattaag
    13741 tgggtagcaa tcagtaaagt actatcttca gaataatttt ttaatatagt cttaagtata
    13801 tatgaccttg cagctgggtc aacaccacct ataggttcat ccaatatata tatgctcgca
    13861 tttcttgaca ttactaaaat aagttgaact ttttctttag ttcctttaga cattgtcttt
    13921 agcttttcat ttacatcaat atctaagctt ttaatcatct catttgctct tctaacatca
    13981 aaatcactat aaaaatcata gaaaaactta agtaaatctg ataccttcat ccaatcattt
    14041 aaataagttc tttctggtaa atatgaaact atttttttag tatctatgga tggtttcatt
    14101 ccatttatca taatttcacc attatcaggc tgtaataatc cattcattag cttaatcatt
    14161 gtacttttac cacttccatt aggccctaac agcccgacta tttttccctt tggtatattt
    14221 aaatcaatat tttttaaaac attcttagtt ccataactct tgtttacacc tttaaattca
    14281 actattccat cttgagaaac attgcttata tttttatcca ttttataatt ctcctttcag
    14341 attttctgta ataagtttta gcatttcatc tctatcatat ccaagctttt ctaaggttac
    14401 ttttaatgta tttatctcca cattagctat ttcctctttc atagctttta ttttttcctt
    14461 atcatctgtt acaaatcttc ctttagttct ctgactataa actaaacctt gtctttcaag
    14521 ttctgttaga gccttttgca ttgtatttgg attaacttcc gcatcttctg ctagtgctct
    14581 cactgtttct aatttagatc ctattttaat ttcccctgaa attattttta actttaaatg
    14641 ttctactaat tgtatatata taggtttgtt attatcaagt tcccattcca tgttcatgcc
    14701 tccttattat attattgtat taatttatta atacaataat ataataatac aaagtatatg
    14761 tcaattactt tttagtaaaa gtataaaaaa tttcacaaaa aaaatgaaca ctacattaga
    14821 gtccattttt aacacaattt attaaattct ttatattaca gattaattac cctcttacca
    14881 agttggatac aattaaatgt tcaaattctt caattggcat tggctttcca aacaaatatc
    14941 cttgtgccat atcacacttt acttctttta aaagtttaaa ctgttcttct gtctcaactc
    15001 cttctgatat gactttcata cccaactctt ttgccatttc tattacttta gtaacaatta
    15061 ttttgcttct tttactatta taagtttcca taataaattt ttggtcaagt tttaagatat
    15121 ccataggaat ttcttttaac atatttaatg atgagtaccc tgaaccaaaa tcatccatag
    15181 atattaaaaa tcctattttt tttaaattat tcattatatc aattaaaata tcaaaattat
    15241 caaatatgat actctccgtt aattcaagtt ctatatactt tgcaggtata ttgtatttat
    15301 caattatatt tttgtatgtt tctataaaat tattgcaata taaatgtatt cttgaaacat
    15361 taactgaaac agtaactgga tttattcctg catcaatcca ttttctaagg tatatacata
    15421 cttcttcaag cacatataaa tctaaattta taacaaaacc gtttctttca aaaaatggga
    15481 taaattcatt tggaggtatt agccctttat caggacgttt ccacctaaca agtgcttctg
    15541 aaccttgtat ctctcctgta ttaagatcta tcttaggttg taagtatata ataaactctt
    15601 tattttctaa acttgaaaac attgtatttt ccatctcttt ttcttttatc tcttggtcat
    15661 gaatcttatt atcataaaat gcatatgaac ttttatgtcc tccctttgcc attttatgag
    15721 gtatattagc cctatctatg attgagttga tatctctatc ttctggaagt ataaaataga
    15781 ttccacaact taaaactaac ttatactttt cttcttgatt attattaaac aagtctaatt
    15841 cctcaaatat agtttttaac ctctttgtta tatcttcttc ttctatatat ttcataatta
    15901 taataaaatt atccgcagat attcttgcaa acatttcctc ttcaaatata ttactcttaa
    15961 taatttttgt taaattacgc aaaatttcat tcccaacttc atatccaaat aaatcattta
    16021 tatatttaaa tttatcaata tctatatata ataaagcata ctttacttga gtatttttac
    16081 atagtatttt gttagcatca attacaaatt tatctatagt atttgctcct gtaagattat
    16141 ctgtatatgc tatcttttgc aatttaattt taaaatttaa tttgtcatat attatataga
    16201 atataagcat catacatatt atagccataa taagtattat ttgtattgca tatttgctaa
    16261 taaactgatt attagacaca atagactcaa agttatttat ggagttgttt ttatagagtt
    16321 tgcataaaga atagactggt gttgtgtatg taatttgtaa aattatacaa ataaataaaa
    16381 aaaacatcaa ttttttttgt aaattcctca taaaaatttc ctcccattaa aacacctcta
    16441 ttttattagc aaataagtac atctcaaaag tttccatttc ttgtaaaatg gatagttttg
    16501 aaaagaaaat attaaaatta taatataaaa ataaaatttt tcagacaatt caaacaaaaa
    16561 taatcacctt ttaataaata attatatgcc aaaataatcc aatttatttt ctattatacc
    16621 tatatgaaat atataaatca atagttaaga caatgaatat gctacattct ataattatag
    16681 tagcagaaaa ttttacaaaa ttaaagtaga ta
//
LOCUS       DAGQWR010000092        16662 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.092, whole genome shotgun sequence.
ACCESSION   DAGQWR010000092 DAGQWR010000000
VERSION     DAGQWR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.092"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            108..1187
                     /locus_tag="MIW95_002609"
     CDS             108..1187
                     /locus_tag="MIW95_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY3151925.1"
                     /translation="MSRVKNVVGLLGTKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            1300..1899
                     /locus_tag="MIW95_002610"
     CDS             1300..1899
                     /locus_tag="MIW95_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151926.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLPPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIAALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(2086..2958)
                     /locus_tag="MIW95_002611"
     CDS             complement(2086..2958)
                     /locus_tag="MIW95_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY3151927.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            3375..4778
                     /locus_tag="MIW95_002612"
     CDS             3375..4778
                     /locus_tag="MIW95_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151928.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            4805..6349
                     /locus_tag="MIW95_002613"
     CDS             4805..6349
                     /locus_tag="MIW95_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBY3151929.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            6380..7771
                     /locus_tag="MIW95_002614"
     CDS             6380..7771
                     /locus_tag="MIW95_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBY3151930.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            7964..9460
                     /gene="abfD"
                     /locus_tag="MIW95_002615"
     CDS             7964..9460
                     /gene="abfD"
                     /locus_tag="MIW95_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBY3151931.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            9595..9870
                     /locus_tag="MIW95_002616"
     CDS             9595..9870
                     /locus_tag="MIW95_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3151932.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            9894..11201
                     /locus_tag="MIW95_002617"
     CDS             9894..11201
                     /locus_tag="MIW95_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBY3151933.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            11221..12339
                     /locus_tag="MIW95_002618"
     CDS             11221..12339
                     /locus_tag="MIW95_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBY3151934.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            12626..13546
                     /locus_tag="MIW95_002619"
     CDS             12626..13546
                     /locus_tag="MIW95_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBY3151935.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            13661..14818
                     /locus_tag="MIW95_002620"
     CDS             13661..14818
                     /locus_tag="MIW95_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBY3151936.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(14903..16402)
                     /locus_tag="MIW95_002621"
     CDS             complement(14903..16402)
                     /locus_tag="MIW95_002621"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBY3151937.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         6423 a         1950 c         3044 g         5245 t
ORIGIN      
        1 aataaaatat gaaaaaattt taggaataaa gtatctgtta gataaaaata agattaatta
       61 gtgatataat ataattaaga taaaatttat tatttggagg taaaatcatg agcagagtaa
      121 aaaatgtagt aggattgttg ggaactaaag gtgttgatgc actatatcta acaaagaaaa
      181 ctaatgtaaa ctatataagt ggttttcctg atgaagaagc atatgcagtg atttgcaaag
      241 atggaaactt tttagttact gatagtagat atatggagct tgcagaaaag gtttgcaaag
      301 attttgaaat tataaattgg cataattttg atagaagtgt ggcaaaggca gttaaaagtg
      361 tatgtgataa agtgggtata aagaaacttg ggtttgaaag aactaatata gtgtttgata
      421 aatatgaaga acttaaaaat ctgattgaga aggataatgg agaattaata cctacagaaa
      481 atattgtaga gactttgaga tatgtaaaag acaaagatga aatcaaaaat acaagaaaag
      541 cttgtgaaat agcggataag gcattagaag aattgattcc acatataaaa gctggagtta
      601 gcgaaattga actggctaca aagttagagt actttatgaa aatgaacgga gcacaaaata
      661 tagggtttga aactatactg atatcaggag caaagacttc attgcttcat ggtaagccta
      721 gtgataagat tatagaaaaa ggtgattttg tacttataga ctatggtgct atgtataatg
      781 gctatatatc tgatacaact agaactttta tagttggagg agcttctgaa aagcaattag
      841 aaatatataa tttagttaag gaagctcaaa atgtaggggt tgagaacatg aaagctggtg
      901 tacatgcgac aattccagat gctgagataa gaaaagttgt caaaaaatat gaagactatt
      961 actatcaagg tataggacat ggagtcggta gggatgttca tgaagaacct ttcattggta
     1021 attatggtga taaaatcata gaagagggat gtataataac aatggaacca ggtatttatt
     1081 tccctggatg gggtggagtt agaatagaag atactgtttt gattactaaa aatggtccag
     1141 aaagacttac taaattccct aaagatttga tgattttaga caaataattt aatttatata
     1201 tattttttta cataaaatat aacataatct cactattaag ctctatttac atattatagt
     1261 aaagagggct taattttatt gtaagaggtg atagtatata tgtataaaaa acataaattt
     1321 ataaaaaata taaaaaaagc ctggtataaa atcaaattaa ttgatagatg ccttataatt
     1381 gtaatggcaa tattaatgtt tcaatctata tataatttat ttgttaatga agtaaattca
     1441 caagatacta caactattga tgtagtagta agaaccactt cagcagctat atttgggtat
     1501 tttttaagtg ctaattttat aaagagacct ttaaggaagt atacaaatac tgatgtatct
     1561 tcaaatcttt ttatagataa taatttaccc ccaaaaggtc aagagtctag tcaaaataat
     1621 ataatgaatg tcaaaaatac cattggattt acttcagaag ctgataatta tgaaaagaaa
     1681 atacctatta ataataatga aggttttgaa gaaggagaaa ctagtgaatt gcaaattatt
     1741 atagctactg taatttgtat agcagcatta acagtacttt ttgttgtaag aaattttaca
     1801 actacaacat cagcttcttt aggtactatt tcacagatga gagattttgt ctcaggatgc
     1861 gtgggttttt tgctaggttg tccaagtaaa cctgattaaa aaagtattat caataaaagg
     1921 tattttaaaa agtatcagta taataatatt tagatttaaa tgtatacaat tataattcta
     1981 taacattcta tattgtattt ttatactgat actctaaact atactattta ttaaaatatt
     2041 ttaacattgt atttaaacaa aatattatat ctgtataata taaatttagt ttatatgaat
     2101 agatgccata tactcaataa aaagtttcat actatgagat atgtattgat ttcttctcca
     2161 aagaactcca atatttgcag taattgcacg ttctaatggt attgaacaga gattttctgt
     2221 attttgaaat atttctttat acaatattgt tgaagccaag tcattttcta gtaaataacg
     2281 tatggtagaa agttgatttg agtgtaaaat tatatttggc ttagcttttt caagtctttt
     2341 tgtaataatc ttatagtgat aagaaccttc agataagatt acaaaaggta cattttttaa
     2401 catataattt gatataatgg gatgttttgc caattcattt ttgggatttg taactaagta
     2461 caattctgtt ttatagattg tctttgaatc tatatcatca taaatatcac tgtcaattat
     2521 accaatacaa aaatcaagtg tagattcttc aatcatttta gctccaacaa atgttggaac
     2581 ttcaaaaatt ttaagctcta tatctggata aattacatta aatctaggaa gtatttttga
     2641 aagcaaaaac gtacctaaaa ctgtaggaat acctattttg agagaagctt ttctcttttt
     2701 tcctaaatca agtgcgtcac attgaaaatc ctcaaattgt tttataaaat gttttgactt
     2761 gtctaaaaaa atttttcctt caggcgttaa ttctatttta ttacctattc tgtaaaaaag
     2821 ttcaaagcca agttcttttt ctaattcttt aattgctaat gttatggatg gttgagaaat
     2881 atgaatactt tccgcagcct tagtcatgtt tttatattta cagacagttt gaaaatagta
     2941 taaatagttt atattcattt ggtattagtt cctttcattt acatattagt gacaagtata
     3001 taattaacaa tagcataagt tttgtatatt gttgtaaaaa aatagaatag ttttaaacta
     3061 tcatagcata agtttttgat attttcaatc tattaagaat agtgctatta ttaaattaac
     3121 aaaagagaat agctggtatg gtttagagta aatcaatcta tattacagtt atatcaacga
     3181 atatcaaaaa attatctatt agaaaataaa aattattttt acaattaaat tgttaattaa
     3241 atcataaaga aaaaagtgat attaacaaga taaaattttt ttaatcagaa atgttaattt
     3301 aataacaaaa tagagtattg gggagaaagg agaaatatat tttttaataa tatttgttaa
     3361 tttaataacg aaaaatgaaa gggagtgatt tagtgaagtt tgaattgatg gattttttaa
     3421 tgaatccttt tgtccttatg tttgctgcag taattacagg aattttattt ggaaaaatta
     3481 aatttgggaa gtttaatttt ggggtatcag gagcattatt tacaggatta tttattggat
     3541 ggcttgcata tagcttgggg aatctaataa tagcaaaagg agaaacagca gcaggatata
     3601 aggcagcaac tgtaatgatg gggaatggaa ttatttcttc tgacttcttt gatttctttt
     3661 taataatatt tgttgcagca gttggtctat tggcagcaaa agatatgaaa gctgtattaa
     3721 agaagtatgg agcaagattt gtaattttag gagttcttat aacttttata ggtggctcta
     3781 tgacttatgc aatgacttta ctatcaagtg ataaaggaag tagtgcttat gaagtttctg
     3841 gcgtttacac aggagcatta acaagttcgc ctggattagc tgctgcttta gaaacagctg
     3901 gtaaacatgc agaagatgta tcaaaagaat ttgaaaaagc ttcaataaaa gataaaaaag
     3961 aaattttaaa agtagtcgac ccagaaggca aattggatgt gaatacaact acatcactga
     4021 ctcaagagca aatagataag tatattgcat atgctgaagc tggtgttggt ataggtcatg
     4081 cagtcgcata tccttttgga gttttaatag taatcttagg tgtaaatttc cttccaaaat
     4141 tatttaggat ggacctaaaa gaagaaagaa gaaaatatga gaaggaaatg aaggaagcaa
     4201 gagatagtgt ttctggtaaa acgataccag aagttccatt taatataata acatttttct
     4261 tgacatgttt agctggatac ttagttggag gaatacatgt atttatggga ccactagggt
     4321 acttcacact aggcgcaaca ggaggttcac taattgtatc tctagttctt ggatatatag
     4381 gtaagatagg ggtagtaaat ttcagaatgg aagaaaaggt tttaaatatt ttaaaacaaa
     4441 tagggctagt atttttctta gcaatagttg gattaagata tggtggaaaa gtagttgatt
     4501 caattatgac atctggtatg cacctagcct tagtagcaat agcagtaggt gttacagcta
     4561 tgatgatagg attcttagtt ggcaaatacg tatttaaatt aaattggatt ttattatctg
     4621 gagctgtctg tggtggtatg acatcaacac caggtcttgg agctgctgtt gatgctcttg
     4681 atagtgatga cccagcagca ggttatggtg ctacgtatcc atttgcatta ttaactaaag
     4741 ttatattggt tatagtttta cacaaacttc caatgtaaat aagattttat atggggggat
     4801 atttatgaat aatgaaggag catttgaaga attaagtgga agattaagaa ataaagaatt
     4861 gtgtgattta ataatgaatg cagatgaagc tgctaaaatt atagaggatg gcatggttat
     4921 tggagttagt ggatttacac cttcaggcta tccaaaagca gtaccattag cagtttcaga
     4981 aagagctaaa agtggagaaa agataaaatt aaccgtatat tcaggagcat ctttaggccc
     5041 agaagttgat ggagcatggt cagaagcagg tataattgaa agaagacttc cttatcaaac
     5101 taattcaata cttagaaata atattaataa aggtgttgtt gattatatag atatgcacct
     5161 aagtcattct actcaatttt taaattatgg aactatacct aaagtagatg tagctatagt
     5221 agaagcactt gcaataactg aagaagggaa tattatacca actagtggga taggtaattc
     5281 tccatctttt ataaaaagtg cagataaagt aatagtagag ataaatcttg caaaaccaat
     5341 ggaaatggaa ggtatggcag atatatatat tacggaaaat ccaccaaata gaaaaccaat
     5401 agaaataaat catcctaagg atagaattgg aacaacatat ataccttgtg gtttggataa
     5461 aattgcagga gtagtaatta ccaatatgca agataagacc cgtccattgg gggttgtaga
     5521 tgaagcttcg aaaaaaatat caaataatat tattgctttt ttaagagaag aagtaaaggc
     5581 aggcagatta tcaaagaatt tacttcctct tcaatctggt gtaggaagtg tagcaaatgc
     5641 agtattatat ggattatgtg aatctgaatt tgaaaattta acttgttata cagaggttgt
     5701 acaggactct atgttggatt taataagaat gggtaaagtt acaatggcat ctactacatc
     5761 tgtaagtcca tctccagaag gattaataaa atttgaaaaa gatattgatt tttttaagga
     5821 taagattata ttaagacctc aagaaattag taacaatcca gaaatagcca gaagaattgg
     5881 tgtaattgca atgaacactg caattgaagt tgatatttat ggaaatgtaa attctactca
     5941 cataatgggg tcaaagatga tgaatggaat tggaggttct ggtgattttg ctagaaatgg
     6001 agcaataact atatttagta ctgagtcgat tgcaaaaaat ggtgatatat catcaatagt
     6061 acctatggta tctcacgtag accacactga acatgatgta atggtaattg taacagaaca
     6121 aggatatgct gatttaagag gtcttgcacc tagagaaaga gctataaaaa taatagaaaa
     6181 ttgtgcccat ccagattata aagaacaact tagggattat ttaaatagag cttgtaaaag
     6241 tggagcaaaa caaacacctc acatattgga cgaagcttta tcatggcata gtaaatttat
     6301 gagtacaggt acaatgaaaa aggctgaaac ttttaaaagt gcattataaa atatttaata
     6361 aattggaggt agattttaaa tggaaaaagc agtggaaaac tttgaagatt taagtaagga
     6421 atacattaat ggttatattg agagagcaag aaaagctcaa agagagtttg aatgttatac
     6481 tcaagaacaa gtagataaaa tagtaaaaat agttggaaaa gtagtgtatt ataatgctga
     6541 atatttagct aaacttgctg tagaagagac tggaatggga gtatatgaag ataaggtagc
     6601 taaaaataaa agcaaagcaa aagttatata taacaattta aaagataaaa aatcagttgg
     6661 tataattgat atagatagag aaactggaat aacaaaggta gctaaaccag ttggagtagt
     6721 agcggcaata actccttgta caaatccaat cgttacacct atgagcaatg caatgtttgc
     6781 acttaaggga agaaatgcaa ttattatcac acctcatcat aaagcaattg gatgtagtac
     6841 aaaaactgta gagatgatta atgaagaatt agaaaaaata ggagctccag aaaatttaat
     6901 acaaatactt gaccaacaat caagagaaaa tacaagaaat ttaatttcat cagcagatgt
     6961 agtaattgcc actggaggaa tgggaatggt aaaagctgcg tactcaagtg gaaaaccagc
     7021 acttggtgta ggggcaggaa atgttcaatg tatcatagat agagatgtag acattaaaga
     7081 agcagtacct aaaatcatag caggaagaat ttttgataat ggtatcattt gttcaggtga
     7141 acagtcagta atagtagcag aagaaatgtt tgataaaata atggatgaat ttaaaaataa
     7201 caaaggtttt atagttagag ataaagtaca aaaagaagct ttcagaaatg ctatgtttgt
     7261 aaataaatct atgaataaag atgcagtagg tcaatcagtt catacaattg caaaaattgc
     7321 aggtgttgag ataccagaag atacaaaaat aatagtaata gaagctgatg gaccaggaga
     7381 agaggatatt attgcaaaag aaaaaatgtg tccagttata tctgcataca agtataaaag
     7441 ttttgaagaa ggtgtagcta ttgcaaaagc aaatttaaat gtagaaggaa aaggacatag
     7501 tgtatctata cattcaaata cagttaaaaa tatagaatat gcaggagaaa atatagaagt
     7561 atcaagattt gttattaatc aatgttgtgc tactagtgct ggaggaagtt tctttaatgg
     7621 tcttgcacct actaatactc taggttgtgg ttcttgggga aataacagca tctctgaaaa
     7681 tcttgattat aagcatctaa ttaatatttc tagaattgct tattatatgc cagaaaatga
     7741 agtccctaca gatgaagaac tttggggata aaacttagac aaaaaatatt cattctaaat
     7801 taaagtagga atatatttta atttagaatg aatttaagac ataaattaaa tttaaattag
     7861 tttattggac taaatttaaa atattaagag acatatagaa gaaggaagta ttataattta
     7921 aaataaataa aaaaatctat aaaagagaag gagtggtttt attatggcat taatgacagg
     7981 agctcaatat attgaaagtt tgagaaaatt aaatactaag gtttatatgt ttggtgaaga
     8041 ggttaagaat tgggttgacc atcctatgat tagaccatct ataaattgtg tagcagcaac
     8101 ttatgactta gctcatgacc cagaatatgc agatttaatg acagttactt caaatatcac
     8161 aggtgaaaaa ataaatagat ttggtcatct tcatcaaagt gttgatgatt tgataaaaaa
     8221 agtaaaaatg caaagactat gtggtcaaaa aacagcttct tgtttccaaa gatgtgttgg
     8281 tatggatgcg tttaatgcag tgtattcaac aacatttgag tgtgataaag cccatggaac
     8341 taattatcat gataattttg taaaatactt aacttatata caagaaaacg atttagtagt
     8401 tgatggagct atgactgacc ctaagggaga tagaagtcta tcacctagtg ctcaacctga
     8461 cccagatatg ttccttcata tagtagaaag aagagaagat gggataattg taagaggagc
     8521 aaaggcacat caaactggtt caataaattc acatgagcac cttataatgc ctactataag
     8581 tatgactgaa gcagataaag actacgctgt atcatttgca gttccttcag atgctgaagg
     8641 tgtgtttatg atttatggaa gacaatcttg tgacaccaga aaattagaag aaggtgcaga
     8701 tgttgattta ggaaataagg agtttggagg tcaagaagct ttagtagtat ttgataatgt
     8761 atttgttcca aatgatagaa ttttcttatg tggagaatgg gatttctctg gtatgttagt
     8821 agagagattt gcaggatacc atagacaaag ttatggtgga tgtaaagtag gagtaggtga
     8881 tgtaataata ggagcagcag ctttagcagc tgattataat ggagcaaaca aagcttcaca
     8941 tataaaagat aaactaatag aaatgactca ccttaatgaa agcttatatt gctgtggtat
     9001 agcatgttca tcagaaggtc acaaaacaga agctggaaac tatcaaatag atttacttct
     9061 tgcaaatgtg tgtaaacaaa atgtaactag atttccttat gaaatagtaa gacttgcaga
     9121 agatatagct ggagggctta tggttactat gccttcagaa aaagacttta aatcagacct
     9181 taaagttgga acaagtggaa tgactattgg cgaagtatgt aacaagtatt ttaaagcttc
     9241 ttcagtggct tcaacagaag aaagaatgag aatattaaga ttcttagaaa atatatgtct
     9301 tggttcatct gctgttggtt atagaactga gtcaatgcat ggggcaggtt caccacaagc
     9361 tcaaagaata atgatttcaa gacaaggaaa cataaatcaa aagaaagaat tagctaagaa
     9421 aattgcggga ataaaaaaag aggaagcttt aaatttataa aaagttgata tataaaggga
     9481 aattatatat taatgtgtat taattaatga aaagatatgt agtataagag atagttataa
     9541 aattatggtg gtttttagct aagtaaagaa atttttaata aggagaattt acacatgacc
     9601 tgtggagtta gattttgtgg gggctgtaac cctagatacg aaagagggaa ggctcttaca
     9661 ataatcaaag agcacttcaa tggtagggta gattttagca tagcacaaga gaacgtcgag
     9721 tatgatttgc tacttataat tggaggatgc gcaactctat gcgcatccta tcaacaatat
     9781 aaagtgaaaa atgaatgtgt taagatgtgg gattttagcc atatagaaag agttataaat
     9841 gtgattgaat cagcaatttg tgaaacttaa aaaagctaaa tggggggatt tcattgagtt
     9901 ggcaagaatt gtatcaaagt aaattatgta gtgctacaga agctgtaaaa cagattaaaa
     9961 acggtgatac tgtagtattt gcacattgtg taggtgaacc tcctgcacta gtagaggcta
    10021 tgattgaaaa tgctgaacaa tataaagatg ttgagattaa acatatggta agcttgggta
    10081 gtggtggata tactgctaag ggaatggaag ctcattttag agtaaatcca atgtttgtaa
    10141 gtggaaatgt aagaaaggca attgaaaatg gagatggtga ttttacacct gcattcttcc
    10201 atgaagtacc aaagttgctt agagaaaaaa gattaaaatg tgatgttgtt ttagcacagg
    10261 taactccacc agatgaacat gggtattgta gcttaggaac atcagttgat tatacttatg
    10321 aagccattaa aactgctaga acagtaattg ttcaagtgaa tgaccagttt cctagaacct
    10381 atggtgaggt agtccatgta tcagagtttg actatatagt ggaaaaatca caacctttat
    10441 ttgaactaca acctgcaaag attggagaag tagaagaagc aattggtaaa aattgtgctt
    10501 cattaattga agatggttct actttacagc taggtattgg tgggatacca gatgcagtta
    10561 tgttattttt aactgataaa aaggatttag ggattcattc agaaatgatt tcagatggga
    10621 ctttggccct ttatgaaaaa ggtgttataa atggtaaata taaaaatttt gataaagaaa
    10681 aaatgacagt taccttctta atgggtacta aaaaattata tgactttgca aataataacc
    10741 ctgcagtaga ggtaaaacca gtagactatg tgaatcatcc agcaattata atgaaacaac
    10801 ataagatggt ttctataaat tctgctatac aagttgattt aatgggacag gtagttgcag
    10861 aggctatggg acttagacaa tttagtggtg ttggaggtca agttgacttt atacgtggag
    10921 tgtctatggg ggaagatgga aaggcaatta tagctatgcc ttcaataact acaaaaaaag
    10981 atggtacagt aatttcaaaa atcgtatcta ttgtggatga aggtgcacct ataacgacat
    11041 caagaaatga tgttgactat attgtaacag aatacggtat agcagaatta aaaggaaaat
    11101 ctcttagaga aagagcaaga aatttaataa atatagctca tccaagtgta agagaaagtc
    11161 tagcagtaga atttgaaaag agatttaagg agaaatatta ataatagagg ggtgttaata
    11221 atggaagcac ttagagtagt tccaaagata ttttattttg atacatttaa agaatttaat
    11281 gaagaattta aaataggaaa aaatgattta gtaattacta atgagtttat atatgagcca
    11341 tatatgaaac ctcttggaat agatacaaat ttaatttttc aagaaaagtt tggaacaggt
    11401 gaaccatctg atgaaatgat agactccatg acaaaggaaa tgaagaaata caattttgat
    11461 agaattattg cgtttggagg aggaacaata gttgatatat gtaagatatt agcacttgat
    11521 gttccagaaa agtctattga tttatttgaa ggagaagtat cacctaaaaa agtaaaagag
    11581 ttagtagtag ttcctacaac atgtggtaca ggtagtgaag taacaaatgt agctattgca
    11641 gagttaaaat ctaaacatac taaaaagggg cttgcagtag aagaaacata tgcagactat
    11701 gcagtgttaa ttccagagac agtaaaaggg ttaccttaca aattctttgt tacaagttcg
    11761 gtagatgctc ttatacatgc tattgagtct tatctttctc caaaagcaag tccatttaca
    11821 gagatgtatt ctctacaagc aataaaaatg attatggatg ggtataagaa gatagttgat
    11881 aaaggagaag aagagagatt taatcatctt agagactttg tacttgcatc aaattatgca
    11941 ggaattgctt ttggaaatgc tggttgtgca gcagttcatg cactatctta ttcaataggt
    12001 ggagctttcc atgttgcaca tggtgaagca aattatcagt tctttactga agtatttaag
    12061 atgtattcta gaaaaaaacc taatggaaaa attataaaat gtacaaaaat tttagcagat
    12121 gcccttgaat gtgaccctaa ctgtgatgtt tatggagaat tagaaaagtt tttaaataaa
    12181 ttaatagcta aaaaagcttt gagagaatat ggaatggtgg aaagtcagat agatgaattt
    12241 acagatagta caatagcaaa tcaacaacga ctacttgcta ataattatgt agagcttagt
    12301 agagaggaaa taagagagat atttgcaaat ctttattaat acaatttttt atttatatgt
    12361 atagtcaaca taaacaatgc taattatatt tatgtacaaa taaaaataag ggaacttaaa
    12421 ttttaattaa agttaagttc tcttattttt atatggagtt aaatataagt atatttttaa
    12481 tttaaaatat gcttatattt aagtctatta ctttatttga aacaatttgt agaatttcaa
    12541 attccttgac agtaataagt aagtaattgt ataatcaaac tgcaatatat taataatatt
    12601 agtgaaaatg aaaggattat aaaatatgta taaatcagaa aatttttcaa aaaaatctgt
    12661 attaataaat tcatttttaa gtaaattttt aaaaataatt ggatggggtt tggtaagctt
    12721 atttacatta tcagccttag tactttcaac tatttccatt ataattggat gggctgtaat
    12781 acttcctatt caagtatgtg cagtattttt acttatagga ggagtttgta tttttaaagg
    12841 taagagatta ggagaccaaa tttctcgtta taaaaaatat tgtacaatta taaacaatag
    12901 aaatattatt ccagtagaac ttattgcaga aaacacatct aaatcattaa attttataat
    12961 aaaagatgta caaaagatga tagacaaaaa ctactttata aatacttata ttgataaaag
    13021 aaataatcag attgtgttaa caaatgaaga ttttattcca ccaagatatg aagatgtagc
    13081 atatgaagtc aaagaagaag aaaagactga tgaatccaat gaagttgata caattataaa
    13141 taaaggaatg aattatctaa cacaaataaa agaagctaat aaaaatatta aatcagaatc
    13201 aatgggcgat aaaattgtac aagtagaaga tgttacaagt aaaatatttg atgttgtaaa
    13261 acatgaccca tcaaaactta ctcaaataca aaaatttatg gattattatc ttccaaccac
    13321 tttaaaacta ttaaattcat atcatacatt ggaagaacaa ggaattgata gagaaaatat
    13381 aacaacaact atggaaagta ttgaaaatac aatgaatacg attgtagtag cttttgaaaa
    13441 tcaattggac tatctgtttg aagatgaagc cattgatatt tctacagata ttactgtttt
    13501 agagaatatg ttagtccaag aaggattaac gagtggtaaa ttttaacttc aatagaaaaa
    13561 atattctgtt atttttaaac aattaattag tcataaagta taaaatgtgt attatataag
    13621 ttaatcagta ctttatgaag gaagaggaga gaaagataca atgaataatt tagatgatat
    13681 accagttatg ccaactttga cacttgaccc atttggagaa gaatcaaaca ctaatgatat
    13741 agataattct gacttattaa tgaaaaagga tgaaaaagac ccagaagaag aaaaactgtc
    13801 tgaatctgaa cgcaagatgg taaaggaatt tgcggaaaaa atagatatta caaatacaaa
    13861 tatgattttg caatatggag ctggtgctca aaaaaaggta gcaagttttt ctgaaacagc
    13921 tttaaagagt gtaaaaacaa gagatttagg agaagttgga gatatgctta caaatctagt
    13981 tactgattta aaagcatttt cagcagatga aaaagaacaa agtggttttt taggaatttt
    14041 taaaaaggca aataataaaa tttcaaattt aaaaactaag tatgatagtg cagaggtaaa
    14101 tgtggataaa gtaagtaaag agcttcaaaa gcatcaggtt aaaatattga aagatatagc
    14161 aatgcttgat aagatgtatg aattgaactt agcttactca aaagagctta ctatgtatat
    14221 tatagcagga aaacaaaaat taaaggatat gaaagaatat gaaatgccaa agttgagaga
    14281 aaaagctcgt ttatctggtt caacagaaga tgctcaatct gtaaatgata tggtttcact
    14341 ttgtgacaga tttgaaaaga aaattcatga cttagaatta actagaatgg tatcgcttca
    14401 aatggctcct cagataagat tggttcaaaa tagtaataat ctgatggcag aaaagattca
    14461 atcaactata gtaaatacca ttccattatg gaaaaatcaa atagtgcttg ctcttggaat
    14521 ttctcactta aatcaagcta tgaaagcaca gcgtgaagtt agtaatatga caaatgagct
    14581 tttattgaag aatgcagaaa ctttgaagat gggaacaata gaaacagcaa aagaatcaga
    14641 aagaggaatt gtagatatag acacaattaa gaaaactaat caatctctaa tatctactat
    14701 tgatgaggta gttaaaatac aacatgatgg tagaataaag agacaagaag cagaagttga
    14761 attatcaaaa atagaaaatg aacttaaaag taagcttctt gaatttacag ttaaataaaa
    14821 attatactaa tataaaaaga gactgtctca aaatagagat aagtctcttt ttcattgttt
    14881 aaatgattaa agtatattta tattataaat ttctattatc ttttaacata acatcatgag
    14941 ctccaccttc aactatacta gtagaagata caagtgtaag tttagcattt ttttgaagtt
    15001 caggaatgct aagtgctcca cagttacaca tagtagaacg aactttactt aaagaaagag
    15061 ttacattgtc tttcaaagaa ccagcataag gcacataaga gtcaacacct tcttcaaaag
    15121 aaagtttctt atctccacca aggtcatatc tttgccagtt tctagcacga gcagaacctt
    15181 ctccccaata ctctttcata taactaccat ttatattaac tttgttagtt ggactctcat
    15241 caaaacgaga gaagtatcta ccaagcatta taaaatctgc acccatagct aaagctaaag
    15301 ttatatgatg gtcataaacg ataccaccat cagagcaaat tggaatataa atacctgttt
    15361 tttcaaagta ttcgtctcta gcttgagcaa cttcaattat agaagtagct tgtccacgtc
    15421 ctataccttt ttgttcacga gttatacata tagaaccacc accaatacca actttaacga
    15481 agtcagcacc agcttcagct aggtaaagga atccctcgcg gtcaactaca tttccagctc
    15541 ctaccttaac tgtatcacca tatttttcac gaatgaaatc aagtgttatt ttttgccact
    15601 cagaaaaacc ttctgaagaa tctatacata aaacatcagc accagcttca actaaagcag
    15661 gaacacgttc agcaaagtct ctagtattaa taccagctcc aactacatat ctcttagaag
    15721 aatctaaaag ttcaagtgga ttttctttat gagaagagta atctttacgg aataccatat
    15781 acataagatt accattatca tcaagtacag gaagagaatt aagtttgtgg tcccatataa
    15841 tgttgtttgc ttcctttaaa gtaacatctt tgttagcata aactatagat gacataggag
    15901 tcataaattc actaacttta gtatctaaat ccatacgact tatacgataa tcacgacttg
    15961 caacaatacc caataattta ccagttgaag ttccatcttc agtaacagct actgtagaat
    16021 gtccagtttt ttcttttaag tcaagtatgt cttttaatgt gttatcaggt ttaatatttg
    16081 aatcacttac aacaaaacca gctttgtggg atttaacacg tgctaccata gcagcttcat
    16141 tttcaatagt ttgagaaccg tatataaatg aaataccacc ttctttagca agtgcaatag
    16201 ccattttgtc atcagaaaca gattgcataa tagcagaagt caaaggtata ttcatataaa
    16261 tatctgcttc ctcgcccttt ttaaatttag taacaggtgt ttttaaactt acattagcag
    16321 gtatacaatc agtagaagag aaacctggta caagaaggta ttcactgaaa gtatgtgatg
    16381 gtgtatcaaa ataaaacgcc attttttaat cactccttat atttttatac tttttataat
    16441 tgatagtatt aattataact tattctttaa ttttattaaa gtattttgtc aaatattgag
    16501 atttttgaaa aatttaaact attttaatta aatttgttta atatatattg ccttataggg
    16561 taaagttgta tattaaataa tgtttagtgt ttaatagtag ctaatttaaa attaatcttt
    16621 agtgattcat ataattacat ttgtatagtt tatattgata at
//
LOCUS       DAGQWR010000093        16617 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.093, whole genome shotgun sequence.
ACCESSION   DAGQWR010000093 DAGQWR010000000
VERSION     DAGQWR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16617
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.093"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            34..2721
                     /locus_tag="MIW95_002622"
     CDS             34..2721
                     /locus_tag="MIW95_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBY3151938.1"
                     /translation="MSLLFIIMGSVLLLFIIKDIEKYETLLHAIYTIFLVATGVIIFN
                     TTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIMEINITISAITDLFPILGV
                     YLSFKFVKDNKQIYSSIILFIATATLTITSLFIISGICDYLGNGKSQYALGVIISIFI
                     IIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVPNLLHIVIDNRNVERILS
                     QIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESLKEKNKKLIYETEKIEEL
                     KGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNTFGDYKEQDKLTDKIKNF
                     NDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQTEGVLCVLEDKTKKKEY
                     ERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIFKNNLESIDFSLVCKSEE
                     SIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRKISQIKLEESQSSYSRFI
                     DILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIKLLMNANTEEKMKFTDNM
                     AKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRYIMLIIKDLVHKKYSEQA
                     EKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDSFYRNEKIKDYNNNIKSH
                     VDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSVIEDLFMNIEEYALRKDI
                     KILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWIYVSIHYKIDKVIISVKD
                     TGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNLVSLHGGDIEVVSEVNKG
                     TEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(2840..3784)
                     /gene="manA"
                     /locus_tag="MIW95_002623"
     CDS             complement(2840..3784)
                     /gene="manA"
                     /locus_tag="MIW95_002623"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBY3151939.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            3957..5318
                     /locus_tag="MIW95_002624"
     CDS             3957..5318
                     /locus_tag="MIW95_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY3151940.1"
                     /translation="MELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSIVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            5406..7295
                     /locus_tag="MIW95_002625"
     CDS             5406..7295
                     /locus_tag="MIW95_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY3151941.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIYCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            7310..7765
                     /locus_tag="MIW95_002626"
     CDS             7310..7765
                     /locus_tag="MIW95_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY3151942.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            7824..8141
                     /locus_tag="MIW95_002627"
     CDS             7824..8141
                     /locus_tag="MIW95_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBY3151943.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            8166..9428
                     /locus_tag="MIW95_002628"
     CDS             8166..9428
                     /locus_tag="MIW95_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBY3151944.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFIDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            9533..10597
                     /locus_tag="MIW95_002629"
     CDS             9533..10597
                     /locus_tag="MIW95_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY3151945.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKIGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKIIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            10651..11829
                     /locus_tag="MIW95_002630"
     CDS             10651..11829
                     /locus_tag="MIW95_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBY3151946.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKNIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(12020..13078)
                     /locus_tag="MIW95_002631"
     CDS             complement(12020..13078)
                     /locus_tag="MIW95_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBY3151947.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTIFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINEIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            13145..13516
                     /locus_tag="MIW95_002632"
     CDS             13145..13516
                     /locus_tag="MIW95_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBY3151948.1"
                     /translation="MKKKDILLILGILVVIIACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            13553..14227
                     /locus_tag="MIW95_002633"
     CDS             13553..14227
                     /locus_tag="MIW95_002633"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBY3151949.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            14476..15315
                     /locus_tag="MIW95_002634"
     CDS             14476..15315
                     /locus_tag="MIW95_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBY3151950.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            15558..15654
                     /locus_tag="MIW95_002635"
     tRNA            15558..15654
                     /locus_tag="MIW95_002635"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15592..15594,aa:Sec,seq:tca)
     gene            15805..16512
                     /locus_tag="MIW95_002636"
     CDS             15805..16512
                     /locus_tag="MIW95_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBY3151951.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
BASE COUNT         6618 a         1734 c         2821 g         5444 t
ORIGIN      
        1 gatgtaaaac cagatataaa agaaagagaa gacatgagcc ttttatttat aataatgggc
       61 tcagtacttt tattatttat tataaaagat atagagaaat atgaaacttt attacatgcc
      121 atatatacga tatttttagt agctacagga gtaattatat ttaatactac aaaatttaaa
      181 gtagattcat tttctacatt tttaggatta atttttatag ctactggagt attggagtgt
      241 atatatttat ttaattcctt ggggattaaa actaagtcta taatggagat taacatcaca
      301 atatcagcaa taacagattt atttcctata ctgggtgtat acttaagttt taaatttgtt
      361 aaggataaca aacaaattta ttcaagtata attttattta tagcaactgc tacattaaca
      421 ataacatctt tattcataat atcaggaatt tgtgactatc tgggaaatgg taaatcacaa
      481 tatgctttag gagttataat atctatattc ataataatta tttctatcat tagtggaatt
      541 gaattaaatt caagttcaaa taaaggtaaa tgggaatatg atgaaaaaaa gatttttaac
      601 agaataatag taataataat tttatcaaga gttccaaatc tattgcatat agttattgat
      661 aatagaaatg tagagcggat attatcacag ataataataa atgtagcctt atattattta
      721 tataattaca tagtatctaa gaatattaag aaaacagcac tggaattgca tgatacgaat
      781 gaagaattaa caaaaaaaac ggaaagttta aaagaaaaga ataaaaaatt aatctatgag
      841 acagaaaaga tagaggaact taagggaata cttatgaaaa gagaatctag gctacaatct
      901 acattagatg tagcagtaaa tagcatagta gtgtttagca aagatggaga aattacatat
      961 gcaaataaga gctttagaaa tacttttgga gactataaag aacaagataa attgactgat
     1021 aaaataaaaa acttcaatga tttgatagaa agcattcatt atgtgtttgt tcatgagaag
     1081 aatgcagaga aacttattag tacaagcgat aataaagttt atcaagctat atttgcacct
     1141 ttaattattg cttcacaaac agaaggagta ttgtgtgtac tagaagataa gactaagaaa
     1201 aaggagtacg aacgcaaact tattgaagca aataagagaa gtaaagattt tttagaaagt
     1261 attggagatg gcatagtagt gcttgaaggt aataacaaaa tatatgttaa taatgcatgt
     1321 agggaaattt ttaaaaataa tttagagagt attgattttt cattagtatg taaaagtgaa
     1381 gaaagtatag aaaaaagata tgtaatagat ggtgaagtaa agtatgtaga gatgagtttt
     1441 tctcagtata ctaatgctgg tactaataaa actattattg taatacgtga tacaacctct
     1501 cgaaaaatat ctcaaataaa acttgaggaa agtcaatcat catattcaag atttatagat
     1561 atattacctg atggcatatg cttattaaaa gaagatttaa gtataaacta tgcaaataag
     1621 tcgtttttag atatgctagc ttttacaaac atagatgata taaaagattc taatattaaa
     1681 ctattaatga atgcgaatac tgaagaaaaa atgaaattta cggataatat ggctaaagtt
     1741 ttaaatgaaa atagaagtat acttttactg gaatatgaat tgataaatag ttatgatgat
     1801 atagtagaag tagaattaag tgcattacct tttgctatat acaatactag atacataatg
     1861 cttattataa aagatttagt acataagaaa tattccgagc aagcagaaaa agaactgctt
     1921 gaaagattta aaacagataa aatcaaaacg gagttttttg caaatatgtc tcatgaatta
     1981 aaaactccac taaatgttat atctagttca aatcaactgg tagattcttt ctatagaaat
     2041 gaaaagataa aagattacaa taacaatatt aaatctcatg tggatttagt tagacaaagt
     2101 tcttataggc ttcaaagatt aataaataac attatagatt taacaaagat ggagtctggg
     2161 ttctatacac taaaacttgc gaaatacaat attgtatctg taatagaaga cttatttatg
     2221 aatatagaag agtatgcact tagaaaagac ataaaaattc tttttgatac agaccttgaa
     2281 gaaattaatg tttatataga caaggttgaa attgagagaa ttatgttgaa tctattgtca
     2341 aactgtatta agtttactga taatggaggc tggatatatg taagtattca ttataaaata
     2401 gataaagtaa tcataagtgt aaaagacact ggagttggaa taccacaaga taaattagaa
     2461 ttgatttttg aagaattttc acaagttgat aagactttat ccagaaatac tgaaggaagt
     2521 ggaatagggc ttgctatagt taagaatcta gtaagtcttc atggtggaga tatagaagta
     2581 gtaagtgaag tgaataaagg cacagagttc ttaatttcac ttccaatgaa gggatttagt
     2641 agtgaacatt acacagaaga taagagaata tataatattc aagaaaaaat aaaaatagaa
     2701 ttttctgata tatactatta aaaatgatta tgagcaaaaa taaaataaaa atactatttg
     2761 tttaaatatt taagttgtaa gaaataggct atcataaggt aaattattat gatagcctat
     2821 ttttagctag gttttatatc tatatgtgag aacatataat ctctaaatta ccttcaaaac
     2881 taaaatctcc aaagtcgttt ggcagtataa aatgcatacc cttttttaaa ttatattcta
     2941 cagaattgtg tataagtttt ccatttccat ccaacacact gtataaagaa aaaggtgtat
     3001 tgtgtgtaaa attacatttt ccaaaaacat ctaatttata aacactaaaa aattcgtttg
     3061 acacaaatgt tgtgcattta aagtctgatg ttgatacgat tttatagtca gtatcgaaat
     3121 ttatatgagg aacattcgta acatctattg atttttgaac atgaagttca cgcttattac
     3181 ctgaattgtc agttctatca taatcataga ctctataagt agtatctgag ttttgttgag
     3241 tttctaaaat caatgttcct ttgcatatag catgtatagt accacttgga acatagaaaa
     3301 agtctccctt ttttatatta acctttctaa gtagtttgtc ccattcatta ttatttacca
     3361 tatttacaaa ttctttatga gattttgcat tatgacctat tataatttct gcatcatcag
     3421 aacaatctat tacataccaa cactctgtct tacctaactc tccattttca tttttcttag
     3481 catattcatc atttggatgg acttgaactg ataaattatc atttgcatcc aatatttttg
     3541 taagtaaagg aaacttatca ccaggtgtgt ttccaaataa ttctctattt ttattccaca
     3601 attcagatag ttttttacct ttatattttc cattttctat taagcaatct ccatttttat
     3661 gagagcttat agcccaacat tcaccagttg ttggcgaatc tatttcataa ttaaatttat
     3721 ctttaagtgc agtaccaccc catattctat ccataaaaat aggttttaaa aataatggtt
     3781 ccataatcct gttcctccta tgatattaaa tttatcctac aaataattat atataaatag
     3841 attataaaat caatagtaaa aaagttttgt cccttgaaat gacataaagt ataaggagtt
     3901 attaaataag ataaactatg atatataata taaagttcta aattggagtg ataattatgg
     3961 agttgtttaa ggcagaaagc aaaaaatatg aaaaagaatt tttaaaatta ttggagcaat
     4021 gggttagtat accttcattt tatgatagaa aaactgtatc taaagatatg ccttttggaa
     4081 aaggagtata tgatgcatta aactggtttg aaaatctagg tagagaaaac aatttcaaag
     4141 taaaaaatat agataatcat gctgttcaaa ttgaatatgg aaatggtaaa gaatatgtag
     4201 atatatttgg acattgtgat gttgtaaatc caggtgaagg atgggatagt gaaccattta
     4261 aactaaatat tataggtgat aaattagttg ctagaggtgt atctgataat aaaggaccta
     4321 tgatagtgaa ttttttagca ctaaaaatga taaaggattt agatattaat ttaaaaagaa
     4381 aggttagatt aatagctgga ggaaatgaag agagtggatt taaatgtata aagcattatt
     4441 atagcaaaga gccatatggt gtatgtggat ttactccaga tgctaaattt cctgtattaa
     4501 atggagaaaa aggtggagca ataataaagt taatttcaaa tatagacgat aaatctttat
     4561 atataagtgg tggaatagag tttaatacaa taccagataa agtttatata aagaatgtag
     4621 aaaaattggg taaagataat atatgttttg atattaacaa tatatcgata aattatgata
     4681 atggcaatta tattgtgtat ggaaaaggtg gtcattcatc taaacccgaa aaatcaataa
     4741 atcctatatt agctacaatt aaacttttgt cagaaaatat tgatgaaaaa tggacaaaag
     4801 atttatataa gttgataaat caagataata taaatggaaa cttatttggt ttaaatattg
     4861 aggggaaatg tgggatttta tcaatagtgc caacaataat aaatatagta gatggaaaat
     4921 tagaagtagt tttaagcgtt agatatccag aaatattaac cattgaagat ataataaaaa
     4981 aatttaattt gtatatggag caaaataata ttaataaatt tgagcttatt ggggaaaatt
     5041 taaagcaagc aaattatata gatagaaatt caaaacttgt aagaagtttg catgatatat
     5101 atataaagta ttctggagat ttgaaaaatg atgttagagt aactagtgca ggaagttatg
     5161 cttctgaaat gaataattca gtaatatttg gttgtgaatt tcctgatggt agctttggca
     5221 atgtccatag tgcgaatgaa tttgctagct tagatagatt tataactgca ataggtatat
     5281 atgcagaggc aataattaca ttatgtaata aaatataaat acaaaatgtc ccttatatag
     5341 acatattata tatacaatta aaattttact tgatatataa ttgaaatcaa agagaaggtg
     5401 atacaatgat aactaagaaa catgcaataa tagtaaagag tttaagcaat aaagatggat
     5461 atatgacatc aaacgaatta gctatcaaat tggatgtgtc tactaaaact attaaaagat
     5521 atatagcaga tttaaatagt gttttaagca aatatgattt ggtaatagct tcttctagag
     5581 gtataggata caagttaagc ggttctaaaa acaatatagc cagagctgtt aaagaagcaa
     5641 ataaatatat tgatggtttt ttagatgact ctgaagagtc aagaatgtca aatataatat
     5701 gtatgcttat taatagaaat tatatgagta tagaagcaat ggcagaagaa ttaaatctaa
     5761 gtatagccgc tataaataaa ttgtctagta agcttaaaag aaaacttgaa aagtatgact
     5821 tagttataaa atctaaacct tactatggaa gctatataca tggtgaagaa attaatataa
     5881 ggcaattaat tactgactat gcaataaaat tagatgaaaa aagtaaagtg aaggtgtttt
     5941 tagatgacat tagtgaaaac gatatatact gtatagaaag tattttagaa aagcacttaa
     6001 gagaaaaaga tacaataatt tcagataaag attttaatct tttgctttca aaaataattg
     6061 tttctgtatt taggagtaag agaggtcata gtaacaatat aaacttaatg gatacaagtt
     6121 atagatttca taactactac tttatagaaa atctaatgaa agaaatatct gataaaattg
     6181 gatttaaact tattgaagat gaagtaattt atatttcaaa ttattctggt gtaatagctt
     6241 ataaaggtac acaaggaaga aaaaacacaa gtgaaattga ggaacgaata agtagtgtta
     6301 taagtagtgc acttcaagat atattcctta tttcaggtag tgattataca aaagatgatg
     6361 agtttatggt tgcaatctct gaccatataa agagattttt aaatagagct agagctaatg
     6421 taaaatcaaa taatccttta cttcatcaaa taaaagagaa gtttcctata gcttttaatt
     6481 tagccgtgtt tttatcaaat aaactagaaa ctgagtttaa tttaaaactt gatgaagatg
     6541 agcttggata tatagctatc cactttgctg cttcaaatga aagaatgaaa aaaaatacta
     6601 gtaagaaaat ttgtatagtt tgtcattatg ggataggaac aggacaactt atttctgaaa
     6661 agctaaaaca gaatataagt gatttaagtg ttgtaggtgt ttatccagtt agatatcttg
     6721 atatggctat aagtcaagat gttgatttga tagtatctac cgttgaatta aaaggatatg
     6781 aaaaacctgt cttatatata gaaaatatat ttgatgatag cttaattgaa aacgtaaaca
     6841 aagcctttta tgaaaaggaa gaaagaagaa agataataag taacatgttt gatgaaaaag
     6901 cttttttcag tataaaggct agtactaaag aagaggtcat aatttcactt tcaaataaac
     6961 taaaagaaag agattttata gaggaaagtt ctataaaaag tataatagat agagaaaata
     7021 tttcatcaac tgaaatagga aatctagtag caatacctca cacaatagtg aagggagata
     7081 aaaaatctat aataggagtt ggaatacttg aaaatccaat aatttgggat aagcaagagg
     7141 ttcagctagt atttatggta ttttttaata ctaaagaaaa gcataatttc tcgatattta
     7201 aataccttta taattttata aaagatgaag gaggagtaag aggaattatt aaaatatgtg
     7261 actttaataa attaatggca ctgataggta attaaggagg aaattaataa tgggaacaaa
     7321 catatttaac aaagaatatg tatttttaaa tgttgatgct aagtcaaagg ttgaagtttt
     7381 aaaatttata tcaaaaaaag ctaaagactt aaatttggca gaagatgaga gcttagtata
     7441 tgaaggttta atggctagag aagaacaatt tactacaaat ttaggagaat ctatagcaat
     7501 acctcacact aaaaatgatg caattgaaaa tccagcagta gtagtattga agtttaatga
     7561 agatgttgtt tggaatgaag gtgaggataa ggttaaatta gcaataagct tacttatgcc
     7621 aggaaaaagt aaagagaata ttcatcttaa attattatca tctctttcaa gaaaattaat
     7681 aaataaagaa tttaaagatt ctcttttaaa aagtgataat gttgaagaaa tatctaactt
     7741 aataaatgaa gctttaggtt tataattaaa tactaaggtt ataaaataaa aaaataatta
     7801 actaaaaata ggaggaattt gaaatgaata aaaaattagt agcattatgt gcttgcccaa
     7861 tgggactagc tcacactttc atggcagcag aagcaataga gcaagcagca aaggcattag
     7921 gttatgaagc aaaagttgaa actcaaggtg ctgatggtgt acaaaatgag ttaacaagag
     7981 atgatatatt aggagcaact atgataatac atgcagtagc aataacacca gaaggtatgg
     8041 aaagatttga tggttgtgaa gtttatgaag ttgaattaca agaagctata aaaaatgcag
     8101 aaggtgttat aaaagaaata gaagaagatt taggaatata ataaatttat tgtggaggaa
     8161 atagaatggc aattaagaaa aaagttataa acagttcagg tgcttctaca acaggaggaa
     8221 ataatgtagg aaaatctact ccatctagtc caactcaatc aaagggaaat ggaaaatgga
     8281 aagaaataag taaacatata atgacaggta tatcatatat gataccagtt ttagtaatgg
     8341 gtggacttat aggggcctta tctcaattaa taccatatgc tatattagga cttgacccat
     8401 ctgtaggtat agttgatgct atgaactcag gagaatttac agggttcaag cttagtcttt
     8461 taaatatagc acaattaatg tcaaatttcg gatttacttt gtttggattt gcgataccac
     8521 tgtttgcagc attttgtgca aactctatag gtggaaaaac agctttaatt gcaggattta
     8581 taggtggata tatagctaat aaacctgtag gtgtaccaca attcattgat ggacaatgga
     8641 ctgaagttgt tccagttgca tcaggatttt taggtgcaat actaatagca ttcataatag
     8701 gatattttgt aaaatggtta aataaatcta taaaagtttc tcataactgg ttagctttta
     8761 aaacaacatt tttaatacca ttaatagcgt cattagcttg tatggtattg atgatattta
     8821 taataactcc atttggtgga ttaattaatg aaagtatgaa aaacttctta acagcagcag
     8881 gagcagctgg agaatatgta tatgcaacag ctttggcagc agctacagca ttcgacttag
     8941 gaggaccaat aaataaagca gcaggattcg ttgcacttgg tttaactaca gagaatgtac
     9001 ttccaataac agcaagaaca atagctatag ttgttcctcc aattggatta ggattaacaa
     9061 cattattaga taaaagatta gtaggaagaa gagtatatga tagacaattc tatcaagcag
     9121 gaaaaacttc tatattctta tcatttatgg gtatatctga aggagcaata ccatttgcac
     9181 ttgagagacc aggttttgtt atacctttaa acatagttgg aagtgtaata ggagctatta
     9241 caggtataat tctaggagca attcaatggt tcccagagtc agctatatgg gcatggcctc
     9301 tagtagataa cttatttgga tatataatag gtatagcagt tggtgctata ttcatagcag
     9361 taggaaatat attctataga aataaattaa taaaagatgg taaacttgtt gtagattata
     9421 tcgactagta tttaattttt actatctata aataactttg gctggtaata tggttttaat
     9481 caatattacc agccttacct atatgattaa ttaaaggcgg tggagataag atatggataa
     9541 gatagaaaaa gtaagagagt attttagaga atatgatata gatggatttc ttataaatag
     9601 tcctacaaac aagttttatg ttggaaattt atttagctct tcaggttatg tttttataac
     9661 gaaagaatca caatatataa tagtagattt tagatatttt gaagaaatta aaagaaaatc
     9721 tagtttgttt aatgtagtat taatggacaa aactagaact cattttgata taattaatga
     9781 tatttgtaga gagcagaata taaaggaaat tggatttgag ggaaatgaag tatcttttga
     9841 tttatataga agtatgagta ataagttaag tgcaacctta aaatcagttg atttatctac
     9901 attaagggaa acaaaaaatg aagacgaaat aaaatatata aaaaaagctt gtgaaatagt
     9961 tgatgctaca ttttatcata tagtagactt tataaagatt ggtatgactg aaaagcaagt
    10021 tgaaaatgaa attgttagaa ttataaaaga tttaggtggt caaaaagaat ctttcgatac
    10081 aatagttgca tctgggctta gaggagcatt acctcatgga aaagcaagtg aaaaaattat
    10141 agagtatgga gattttgtta cttttgactt tggtgctaag tataataatt attgttcaga
    10201 tattaccaga accatttgta tgggtactat aaataaagag ttagaagaaa tatataatat
    10261 agtaagaaaa gctaatgaag agtgtataag agttttaaga ccgggaatga ctactggaga
    10321 gatagataaa gttgcaagag acataattgg ttcatatgga tatgccaata attttggtca
    10381 taatctgggt catggtgtag gaattatggt tcatgaatat cctgccttag ctcctgaatc
    10441 aaatgaagta ttaaaagaag gaatgattgt aactatagag cctggtatat atgttccaag
    10501 tcttggaggc gtaagaatag aagatgatgt actcataaca caagatggat gtatgagatt
    10561 gactacatct actaaggatt taatagttgt aagctaaaaa taaattagat aaatatttta
    10621 taacaataag aaaagaagag gtaaatttaa atgtataatt ttgatgataa accagataga
    10681 gtaagtgaaa agtgtagaaa atgggattta aatattataa gagataaatt tggagatatt
    10741 agagaagatt ttgtaccaat gtggattgct gatatggatt ttaaaatacc aacagaaata
    10801 gaaaacaaat ttattgaagc tgtaagaaga ggtgtatttg gatatacata ctgttatgat
    10861 gaattttatg attcagttat tggttggcaa aaaaatatgc atgaggtaaa tgtagaaaaa
    10921 gaatggatta cattaactta tggaactgta tcaacacttc attatgtagt acaagctttt
    10981 tgtaagaaag gtgatagtat aatattaaac acaccagttt atgacccctt tgaatcatca
    11041 gcaaaaaaac aaggtgtaaa tgtaatttgt aatacattag atgttgtaaa tgatagatat
    11101 tatataaatt ttgataaatt agaagttcaa attaaagaaa ataagcctaa attaatgatg
    11161 ttttgtacac cacataatcc atctggaagg atatggacaa ttgaagaaat gacaagggta
    11221 gcaactatat gtaaagaaaa taatgttatt ttggttgcag atgaagtaca tgcagagcat
    11281 attcattatg gtaaatttaa ttcaatactt aagattggaa aagagctttt agaaaatgtt
    11341 attctactga catcaccaaa taagggcttt aatcttggtg gattaaagac ttcttattca
    11401 atagttataa ataagaatat aagggataaa tttagaaata aattaaagca aaattctata
    11461 acttctccaa atgtgtttgg aataatagga ttgataaccg cttataatga atgtcatgag
    11521 tggttaagtg gtgtaaatga atatataaaa tcaaattatg aacttttaga aacttgggta
    11581 aataaatata ataagataaa attgatgaaa atggaatcat cttacttagc ttggatggat
    11641 ataagtggat taggtataag tgcaagtgag tttacagata aattggctat agatacagga
    11701 gtattattag aagatgggag tcattttgta aaagatggtg agaagtatgt tagaataaat
    11761 ttaggaactc aaaaggaaaa tgtaattgaa gctttaaata gaatggattt atttttaaag
    11821 tctttataaa aatttggttg aattagtatt ttatataaat ttaagctatt aatatggtat
    11881 atacataagt agaatttgtt ttagattact taagtatata ccatatttta tataaaaatt
    11941 aaatttgcat tagtcatgtg ataactattt aaattcatat gtattttgta tgtataatag
    12001 atataattat tatttaaact tattgtttat ttaatttttc aatagaaaaa gatttatctg
    12061 taagtttaaa attatctttt atacccttag ttaaatatac tttcttatcc ttagttatga
    12121 atatagcatc tacattatca atttcattaa ttaaatccat acctttttca agaccaagtc
    12181 caaatgtaga agtggataag gcatcacaat taatagattt atcagaaatt attgtaacac
    12241 tacttagatt gttttcaaaa ggataccctg taaatggatt taacatatga tgatatattt
    12301 ttccgtcttt ttcgataaat ctttcataaa ttccagaagt aacaacagat ttatttgtgg
    12361 tttctatgtt tcctatggat tcaccatttt tactagtggg gtcttgaatg cctattttaa
    12421 aaggtttatc atttttacca tcttctaaag taaatatatt tcctcctaag tttactaaac
    12481 ctttttttac gtcctctgat tttaaatagg ctacaatttt atcagcagca tatcctttag
    12541 ctatggcacc taaatcaatt ttcatatttt ctttggttaa ttttatagat ttattttttt
    12601 catccaatat gatgttctta taatcaataa gaggcagaag tttttgtatc tcagaatcac
    12661 ttggaacttt agctttatct gttccaatag cccataaatt tgataatgga ccaattgtta
    12721 tgtcaaaata tccatctgat aatttggaat attgtatagc ttcttttacg acgaaaaatg
    12781 tatcatcaga aactctgaca aattttttac cagcattatc atttatttta gaaacatcac
    12841 ttcctggtat atgagtgctc attttgttgt caatatatct taaaattgaa tcacattcat
    12901 ttaagatttt atcacttttg gattctttta cattaaatat agtaacttcg tttactgtac
    12961 caaggtagta gctagtttta ctatatttag tattctggct tgaactccta aaaaatgaaa
    13021 ataaaattaa tgctaaaatc aatatcacaa aaataaatgt gatttttttc ttattcatgg
    13081 tatattcctc ctatgaaatt gtgttattat tttattataa cataatttca taggaggaca
    13141 aaaaatgaag aaaaaagaca ttcttcttat tttaggcatt ttggttgtta ttatagcatg
    13201 ttatgggata ataaatgtta taaattctaa aaatgctgga aatattgaga tttatgtaga
    13261 taataaattg tataaaactg tttcaataaa tgccaaagaa gaatttaaaa tagagaatag
    13321 aggggggtat aacatagtaa agattcatga taaaggggtt gagatagtag atgcttcatg
    13381 ccctgataag gtttgcgtac atactggatt tataaacaag ccaagtcaaa gcatagtttg
    13441 tatacctaat agagtgagca taaaaataaa tacaaatgaa aagaatgata atcaagaaga
    13501 tataatttct aaataaaata ataaaaataa ttgaaaggaa ttggtgataa ttatggttaa
    13561 cttaggaaac gattgggatg aattattgaa agaagaattt gaaaaagatt actacttaaa
    13621 cttaagaaaa tttcttatag aagaatataa aacacgccaa attttcccaa atatgcacaa
    13681 tatatatgaa gctttaaaac acacttcata caaagacaca aaagtcttaa tattaggaca
    13741 agacccttat catggagata atcaagctca tggtttagct ttttcagttc aaccacaagt
    13801 gaaaactcca ccatctctat taaatatgta taaagaatta aaagatgatt taggttgttt
    13861 tatacctaat aatggatatt taatgccttg ggcagaccaa ggagtgttac ttttaaatac
    13921 tgctcttact gttagagcgc atgaagctaa ttctcataag aacaaaggat gggaaatttt
    13981 cactgataga gtaatatcta ttttaagtga gcgtgaagac ccagttattt ttgtactatg
    14041 gggaagtaat gcacgtaaaa aagtagagtt gattgataca agtaaacatt atattttgga
    14101 agcacctcat ccaagcccat tgtcagcaag taaaggcttt tttggatgta aacacttctc
    14161 taagataaat gaaattttga aaaagctagg aaaagaacct attaattggc agatagaaaa
    14221 tatataaaag taaaaggtaa caaaatgata acaaaacaac aataaacaat gacattgttg
    14281 taactaatag aaatatgctt aaatagttga aaatactatg tatgcatatt tctatttttt
    14341 tgtcaagttg acaactcttg aaaaaatagt tacaatttag ttacacaaaa aacacaaata
    14401 aaaaaagaaa cttacaaagc agaattttat gaagcaaaat atatagtgta aaaaatttta
    14461 gggaggaaat tttatatgaa ttttaaaaaa ttgagtaatg ctaaagtctc ggtaatggca
    14521 gttgttttat cagttggaat attatcggga tactctgcgc ttaataaaca agtaacatta
    14581 gtagtaaggg gagaagaaag aactatatca acatttagtt ctaatgtagg agacctttta
    14641 gaatctcaaa acattagcta tgatgcaaat gatatagtaa ctttagatga aaatacaaag
    14701 ttatctaatg gagataaaat agaagtaatt gatgtaaagg aaaaaacagt tgtagaaact
    14761 aaagacattc catttgaagt tacagttgtt gaagatggct ctttattaaa gggtgattct
    14821 aaggttaaag aagaaggaaa gtctggtaaa agtgaattaa tatataaaga aacttatcac
    14881 aatggaaaga aagttgaaaa ggaatttgtg aaaaaggttg ttaaagctga gccagtaaat
    14941 aaggttgtaa gcaaagggac aaaggtagaa atgcaagtag cctcatcaag gggagaaagt
    15001 tcaagaagaa tggcttcatc atcatctagt agcagtagta gtaacaatgg taaaaatatg
    15061 agagtggtag caactgcata tactggtcat agtataactg caacaggaac aaaacctaag
    15121 tggggaacaa tagctgtaga ccctaaggtt ataccatatg gaactaaagt ttatatacct
    15181 caatttgaca tggtgtttac tgcagaagac tgtggtggag caataaaagg aaataaaatt
    15241 gatatctaca tgaatgattc aaaaacggtt tataactggg gaagaaaaac tatagatata
    15301 cgtatattaa aatagttaaa taatttatat ttttactgga aattatattt tgattatata
    15361 gagaagaaac tctttataga ttcttaaaat ataatttcca gtttttattt tggatatgta
    15421 ataaaacagg taaattttaa ccatgtatag ataaaagcta tttaaattat aatttactat
    15481 cagataaatc tataaaaatt gttgcaaaga gtcaaaatat atgatattat agatttcgtt
    15541 gagaaggatt tcattatggg agtggatggg tgccggtgtg ccctctagtc ttcaaaacta
    15601 gtatgagggg ttaagagcct cttaggtggg ttcgattccc acgcattccc gccaatatgc
    15661 aaaaggtatc ttttaattaa aagatacctt tttaaattta ttttaagata atttattttt
    15721 aataatatat aatgttttgt attatataat gtaaattttc taatactatt atatatttag
    15781 ataaaaatct aggagtgtgg aaagatggaa aataatttta caacgagaac tagttttcta
    15841 gttggagatg atgggataga aaaacttaat aattcaaata taatagtatt tggagttgga
    15901 ggagttggta gttttactgt tgaagcttta gcaagagctg gagtaggcaa tattactatt
    15961 gtagattttg atgatgtaga tataaccaat ataaatagac aaataccagc cctacattca
    16021 actgtaggaa ggtacaaagt tgatgtcatg gaagaaagaa tattagatat aaatcctaat
    16081 atcaatataa aaaaaataag aagtctttat aataaagata ctagtgacga aatactaact
    16141 gaaagatatg attatgttgt ggatgcgata gacatggtaa gttccaaaat acatctaatt
    16201 gaaacttgtg aaaaaaaagg gttgaaaata ataagttcta tgggtatggg aaataaatta
    16261 gacccaacta aaatagtcgt tacagatata cacaaaacaa gtacttgtcc tctagctaaa
    16321 gttatgagga aagaacttag ggatagaggt atcaaaaagt taaaagtagt atattctact
    16381 gaacaaccaa tagaacttaa gaagaaagta atgaatggta gaaaagtaac acctggtagt
    16441 gtatcatttg tcccatctgt aggaggttta ataatcgcat ctgtaatagt aaatgaacta
    16501 ttaggacaat aagttttaaa tattttaatt tataaatttt atatacaaat ttgaaggcta
    16561 gaagcatgaa tataaagcaa ttttatattc atacttttag cctaattatt ttatatt
//
LOCUS       DAGQWR010000094        16370 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.094, whole genome shotgun sequence.
ACCESSION   DAGQWR010000094 DAGQWR010000000
VERSION     DAGQWR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.094"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(110..2386)
                     /gene="slpA"
                     /locus_tag="MIW95_002637"
     CDS             complement(110..2386)
                     /gene="slpA"
                     /locus_tag="MIW95_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBY3151952.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFAAEDMSKVETGDQGYTVVQSK
                     YKKAVEQLQKGLLDGSITEIKIFFEGTLASTIKVGAELSAEDASKLLFTQVDNKLDNL
                     GDGDYVDFLISSPAEGDKVTTSKLVALKNLTGGTSAIKVATSSIIGEVENAGTPGAKN
                     TAPSSAAVMSMSDVFDTAFTDSTETAVKLTIKDAMKTKKFGLVDGTTYSTGLQFADGK
                     TEKIVKLGDSDTINLAKELIITPASANDQAATIEFAKPTTQSGSPVITKLRILNAKEE
                     TIDIDASSSKTAQDLAKKYVFNKTDLNTLYRVLNGDEADTNRLVEEVSGKYQVVLYPE
                     GKRVTTKSAAKASIADENSPVKLTLKSDKKKDLKDYVDDLRTYNNGYSNAIEVAGEDR
                     IETAIALSQKYYNSDDENAIFRDSVDNVVLVGGNAIVDGLVASPLASEKKAPLLLTSK
                     DKLDSSVKAEIKRVMNIKSTTGINTSKKVYLAGGVNSISKEVENELKDMGLKVTRLAG
                     DDRYETSLKIADEVGLDNDKAFVVGGTGLADAMSIAPVASQLRNANGKMDLADGDATP
                     IVVVDGKAKTINDDVKDFLDDSQVDIIGGENSVSKDVENAIDDATGKSPDRYSGDDRQ
                     ATNAKVIKESSYYQDNLNNDKKVVNFFVAKDGSTKEDQLVDALAAAPVAANFGVTLNS
                     DGKPVDKDGKVLTGSDNDKNKLVSPAPIVLATDSLSSDQSVSISKVLDKDNGENLVQV
                     GKGIATSVINKLKDLLSM"
     gene            complement(2612..4201)
                     /locus_tag="MIW95_002638"
     CDS             complement(2612..4201)
                     /locus_tag="MIW95_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HBY3151953.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGSPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNIDTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYSLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVINGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            complement(4420..6021)
                     /locus_tag="MIW95_002639"
     CDS             complement(4420..6021)
                     /locus_tag="MIW95_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBY3151954.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADRQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASKSNGQPPTITVGSTEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSYDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKNLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            complement(6388..8268)
                     /locus_tag="MIW95_002640"
     CDS             complement(6388..8268)
                     /locus_tag="MIW95_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBY3151955.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSVTCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVDYENINSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKITKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSNSTSNVTVKKLKGADRFETA
                     TKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAVQFIKSNKVSNIYFIGGI
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVVDKIKSLI
                     EK"
     gene            complement(8478..14441)
                     /locus_tag="MIW95_002641"
     CDS             complement(8478..14441)
                     /locus_tag="MIW95_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HBY3151956.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGN
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNIGTDNIEYTIK
                     KLSASRSEIKNKDENVSYYNIVFNNLPVGKYNIEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDEDGNVQQ
                     IPYENKEYMKNLKDKEQQEEVNNEQEEVNQEEVNNKDENKNQTDNNSNNKDENNDKQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEGDNISKDDNSTTSTYSRAALE
                     TYTDVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSLKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESIDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEIRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQ
                     EINKSGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLKTGKNVIQLSKLHSLDIECGGSLYIRYPEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNIGNSY
                     EYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKAKFTDSISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDVSTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPEAETRKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDDKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDPSTSEDETGKIPAGS
                     IIITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNAIDVSNKKVITVSNKVKGNIVRDK"
     regulatory      complement(14565..14650)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(14822..16225)
                     /locus_tag="MIW95_002642"
     CDS             complement(14822..16225)
                     /locus_tag="MIW95_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBY3151957.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILIDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALVASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     DNGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKISDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT         4596 a         3075 c         1823 g         6876 t
ORIGIN      
        1 tatttctcac ataaaaaaga gactaaattt agtctctttt tttgtattcg aaaacataat
       61 ataaaaagac ttctcatata agaagccttt ttatattatt atatttatat tacatactta
      121 ataaatcttt taatttattt ataactgaag tagctatacc tttaccaact tgaactaagt
      181 tttctccatt atctttatca agaactttac ttatagatac actttgatct gaagataaag
      241 aatcagtagc taatactata ggtgctggag atactaattt atttttatca ttatcagaac
      301 cagttaatac tttaccatct ttatctactg gcttaccatc agaattaaga gttacaccaa
      361 agtttgctgc aactggagct gctgctaaag catcaactaa ttgatcttct ttagtagaac
      421 catctttagc tacaaagaaa ttaactactt ttttatcatt atttaagtta tcttgataat
      481 aagaagattc ttttataact tttgcattag ttgcttgtct atcatctcca ctatatctat
      541 ctggagattt acctgtagca tcatctattg cattttcaac atctttagat acactgtttt
      601 ctccacctat tatatcaact tgtgaatcat ctaagaaatc ttttacatca tcatttatag
      661 ttttagcttt tccatctaca actactattg gtgtagcatc accatcagct aaatccattt
      721 taccattagc atttcttaat tgagatgcaa ctggagctat actcatggca tctgctaatc
      781 ctgttcctcc aactacaaat gctttatcat tatcaagacc tacttcatca gctattttta
      841 gagaagtttc atatctatca tctcctgcta atcttgtaac tttaagtccc atatctttta
      901 attcattttc tacttcttta gatatagaat taactccacc agctaaataa actttctttg
      961 aagtatttat acctgttgta ctctttatat tcataactct ctttatttca gcttttacgc
     1021 ttgaatctaa tttatctttt gaagttaata ataaaggagc tttcttttca gaagctaaag
     1081 gagaagctac aagtccatca actattgcat ttcctccaac caatactaca ttatcaactg
     1141 aatctctaaa tatagcattt tcatcatcag agttataata tttttgactt aatgctattg
     1201 cagtttctat tctatcttct cctgctactt ctatagcatt tgaatatcca ttattatatg
     1261 ttcttaaatc atccacataa tcttttaagt ctttcttctt atctgactta agagttaatt
     1321 taactggtga attttcatca gcaattgaag cctttgcagc actcttagtt gtaactcttt
     1381 ttccttctgg ataaagaacc acttgatatt ttccactaac ttcttctact aatctattag
     1441 tatctgcttc atccccattt aatactctgt aaagagtatt taaatctgtt ttattaaata
     1501 catatttttt agctaaatct tgtgctgttt tactagaact agcatcaata tctattgtct
     1561 cttcttttgc attcaatatt ctaagtttag ttattactgg gcttccagat tgtgttgttg
     1621 gtttagcaaa ctcaatagtc gcagcttgat catttgcact tgcaggtgtt attattaatt
     1681 ctttggctaa atttatagta tcactatctc ctaatttaac aattttttct gtttttccat
     1741 ctgcaaattg aagacctgtt gaataagtag ttccatcaac taaaccaaac tttttagttt
     1801 tcatagcatc ttttatagta agtttcacag cagtttcagt tgaatctgta aaagctgtat
     1861 caaatacatc tgacatagac ataactgcag cactacttgg agctgtattt tttgctcccg
     1921 gagtaccagc attttcgact tcaccaataa tactacttgt agctactttt attgcactag
     1981 ttccacctgt taaatttttt aatgcaacaa gtttacttgt agttacttta tctccctctg
     2041 ctggagagct tattaagaaa tctacataat ccccatcacc taaattgtct aatttattat
     2101 ctacttgtgt aaacaataat ttacttgcat cttctgcact aagctcagct cctactttta
     2161 tagtagatgc taaagttccc tcaaagaaaa ttttaatctc tgttatactt ccatctaata
     2221 accctttttg taattgttca actgctttct tatacttgct ctgtactact gtatatcctt
     2281 gatcaccagt ctcaactttc gacatatctt ctgctgcaaa cacaggtgct gcagaagcta
     2341 atactgttaa tcctgacata gctattgcta tattcttctt attcatttct taaattcctc
     2401 ccaacattat attaaaatta catctcttca ttatgtacat agaataatca tccatatatt
     2461 cctaatattt ctcacatctc tataataatc catttttatt ctttttgcaa gtcatttttt
     2521 tgaaattatt atttttccca agcatatgag tttttatacc cttaaactcc aatatttaat
     2581 aacttaaaaa atttgatttt acttatttaa attacttatt taaaaattct tgtgtagcat
     2641 acacttctcc attaggcaat atatataatc caaacccagt atatacaaaa tcactattta
     2701 acatattttc attatattta tctgactttt tccaaactgt aaatattaca tttgctactt
     2761 ctctagcatc ctttgttgta taagtacttt ttgtaacaag tggtacaaaa gctatatttt
     2821 cttcacttct caagccatat ccagtaaata cttcaacaga tttcttacca tttattacat
     2881 gtgaaaactg acctttatct gccatgtact tagaccagga attagaaagt ccttctaagt
     2941 ttttagaaac ttgtaatggc tctttacctt tttcttttct atatgaattt accaaactat
     3001 acatttcact agaaaccatt cttttaaact cactactatt catatcatta gtcttatatt
     3061 caagtacatt tacttctaca cttattgatg tagtctttcc accaatatca gtagctttta
     3121 tagtaacttt gtatttaccc accttatcag tatcaatatt tcctataagc tctggtgtca
     3181 aatcgttacc attgctatcc tttgctacga tattcagttt acttatatca aacttatcac
     3241 cttgataaat atttaaattt ccaacagtta ttgttggtgc acttgcatcc agtgatgatg
     3301 cacttaaaca ttgttctagt gtcttttcat ctattcctcc tagtgctgta atcttatcag
     3361 cacttctaag tacagactta tcactatttc cgtcaactaa tacaataggt gcattttttg
     3421 ctatagaaga accagcaact gcatctacta attgataacc tttagaaaga taaaattctt
     3481 ttgccgaact ataaaaatgt tttataactt ttttattagt ctcaaatcta tcttctccag
     3541 ctaatctaac tgcattagtt tttttaacta aatcattact tattatctcc tctgaaccaa
     3601 gagcataaca ttgcacacct tcttttttct caaatggaac atttttacca ttagttaaaa
     3661 ttataggtga tccatctctt gatgctactg atgacgcact catagcatct gcttcacctg
     3721 tataaccatt agttataaaa accttatcta taggttttat tgaagctatt tcttttgcta
     3781 taagataact tgtttcaatt ctatcattac cacctattct tttaacttct attccttttt
     3841 gctttaattc attttcaact gattttccta ttgaatcttc agttcctatt atataaacct
     3901 tctttacatc ttttaacatt ttttctgtgt ctgatggtat actgtttctt tgtgataata
     3961 atataggtgc tttaactgtt cctgctaatc cacttgcact taatccatca gctagtgtat
     4021 tatctgtatt tactaatact acattttcat atgtttgttt ctgtgcaatt ttggcagctg
     4081 tctcatatct atctttccct tgtatctttt ctatgctcga aagtgcattg atatttggtg
     4141 ctcctgccat tattaatgat attgtcaatc ctattgataa tactctctta tttaccttca
     4201 tcgaactcct cctactaatt tttttatata attcagctaa tcataaatta catcctcagt
     4261 agtgattccc tcaaaatcta taaactacta aaacaaaaaa cacctaaaat accatagcct
     4321 gtaaagcaat aacaaaacag attacaaaaa gtactttaga tgtgattttc attttataat
     4381 atcagtaaat taacaaaaaa acaagattta tggaattttt tacaaactac cttcattgcc
     4441 atttaaaaac tcctgagtag catgcacttg accatcagat aatatataaa gcccaaatcc
     4501 agtactatag aactcatcac taagcatgtt tgcattgtac tctggagact tcttccacac
     4561 ttcaaatata gcctttgcca agttttttgc atcttgtgtc gtttgaacgt tcttagaatc
     4621 tatataaata tatgctatat tttcctcact tctcattcca aactcagaaa atacttgtgg
     4681 agcatttttt ccatctatat aatgtgcaaa tactttttta tccatcatat acttagacca
     4741 tgcatttgcc ataccttcta gcctactaga tactaccaaa ggctctttgc ctttttcttt
     4801 tctataggaa ttgattaagt tatacatttc agtagatacc atttttttaa attctgggct
     4861 gttgtaatca taggatttat catccaacac ttttatttct actctttttc ctgtactttt
     4921 tccccattcg tctgtagctt ttaatgtcaa tatgtatgta cctactctat ttgtatctat
     4981 aaacccatca acttctattg gtaatacctt cccagtgtta tctttagcca ctatgtttaa
     5041 cttgccagtg tcaaactttt cacccttata tatctctgta cttccaactg taattgttgg
     5101 tggttgtcca tttgactttg aagcatttat acactgttgt attaccttct catttatttc
     5161 acctacagaa gttatattct tagctccttc taatacagtt ttatcgcttc catcatttac
     5221 tagtaccatt ggagagtttt tagtaagtgg tgcagctgct attgcatcta ctaattggta
     5281 tccatctgat acatagaatc catctgcact attaaaaaag tagtctatta catttttgtt
     5341 agtttcaaat cggtcagttc cttctattct tactgagttt gtatttttaa ctagagggtt
     5401 actcattatc tcctctgaac ctatacaata tgattgaata ttttttacat caaaaggcac
     5461 actctttcca tctgtaagta tgattggagc tccatctcta gtagctactg atgatacact
     5521 cattgcgtct gcttctccac tatatgcgtt agttaatagt actttatcca ctttttttat
     5581 agttgctatt tctttagcta ttaaatagct tgtttttagt ctatcttctc caccaattct
     5641 tttgacttct atatttttag aatctagttc tttttctact gatttactta tagtatcttc
     5701 tgtacctatt atatatgctt ttttgatatt ttttagacat ctatttgtgt ctgctggtat
     5761 cttattttgt tgtgtaaata gtattggagc ttttgtagct cccgataatc cacttgcact
     5821 taatccatct gcaagtgatt tttctgtatt tactaaaatt actgtttcat aagtctgtct
     5881 atcagctatc tttgctgctg tttcatatct atctttaccg tatatttttt ctactactga
     5941 tgatgcattt acacttttga agtttactaa gattaatgat actgctagac ctaatgataa
     6001 tatttttttg ttcattttca tatccccctg atgattttta tattttatat ttatattgtg
     6061 attattttct atttattttc tctttatttt ccatatatta tagagtatat atgatatgaa
     6121 tatatctcgt ctatatatta gtgttttatt ttattaaatt taaaaaatta tttttaactt
     6181 ctttttaaac atattcaaaa ttgaatttat atttatatat ataaaagata tctgctttat
     6241 ttaaaattaa acttaatata gctcaaatta ttatacattt taacctcaat attattaaat
     6301 tgttaagata atgaaaataa aataagaatg catctaaata tataacttta tatctaaata
     6361 cattcttatt ttcgatttac ttaatattta tttttcaatt aaactcttaa tcttatctac
     6421 tacagatgag gctattcctc cacctacttc aaacaacttt gatgaatttt taccttttaa
     6481 tgatgattct tgtgatttat ctaacgtatt tgtagcaagt ataactggtg aattgttttt
     6541 agctgctaat ggtccaactg ctaaagcatc tataagttta tcttgatttg caactacaac
     6601 tccatctagt ttagattgag agtagaattt atctattact ttggcattcg tctcttgtct
     6661 agtttgacct gccaccctat tgtttgatat atctttgttt gctatttttc caacttgttc
     6721 aatagccttg ttagatattt tttctattcc accaataaag tatatatttg acaccttatt
     6781 tgatttaata aattgtacag cttcattatc tacaccatct ttttgtgtaa gtactattgg
     6841 agttttttct ttaccggcca gtgatgctat tgataatgaa tctgcttctc catttcctgc
     6901 ccctatatac aatttgctta cattattcgt cttatctatt tcttttgcta tattaataga
     6961 agtttgatat cttgtatctc cacctattct ctgtactgat atttttgaat ttacagcttt
     7021 gatagcttca acaactgaat ttggcattga gttgtttcca cctatcacaa ttacttttga
     7081 tggatttagc cttttcaatt cttctacagt tttttgagga agcttttcat ttgatgataa
     7141 aagaatcggt gagtttttta cactagcaag tggtgtagct gtaagcccat ccaccatgct
     7201 tttatcttct ccatttacta ttacaactgt atctgatttt gtccaaccac tttgagatat
     7261 tttggtagct gtctcaaatc tatcagcacc ttttaatttt ttgactgtga cattagatgt
     7321 agaattactt gaacttcctc ctccagaact tccaccacta gacgagccgc ctgaattact
     7381 tccaccacca ttatttccac cattgccatt atctggtggt aattcatccg gttttgataa
     7441 ttcattttca actatgtatt ctaaactttt tggaagtgta acttcaacat atttatttat
     7501 tgtagctttt cttcccttag ttattttttg tgctaatcca agttttaata ttacatttga
     7561 accactttct tgtgtttctt tactaacaat agaatagttt atcctaatat tgtttaataa
     7621 atattcaagt gcttcacttt tctctacctt atacttctca gggtcatttt tactagaacc
     7681 agttccacca attggtttta cttcactctc attaataagg gtaaatatta atccttcatc
     7741 agagtttata ttttcataat ccactgtttt gttgttatat gttacaattt ttaaagctgg
     7801 tttatcgctt gttccttttc ctaaaacttt atagtcagca tttgagttac tgccagattt
     7861 ttttacagat attcttccta tattcaatct atttccgtct agtaaatctt catttgatgt
     7921 aacatatata tctaatgtag ttgtattact tgatttgttg tattctactc tacaagtagc
     7981 atttgcattt ccacttttta tctgagattt tatattatca gaccaagtaa tcttttcttt
     8041 ctgctcatta tctactactt caacctcacc ttgtactttt atactggctt gaaatgaata
     8101 tactatgttt tctactgagt ccacagatat atctatagta tttactttct catacgtttt
     8161 tgttccttgt aaaactggag ctgcatatgc acatgtaaca ctaccagtta cagctactgc
     8221 catagttgat actgctatag ttcttataaa agattttctt ttactcatta acacccgtcc
     8281 ttatctatca ttaactaagt ggataaattt acttatccac ctagctttta tatttaattt
     8341 ataaattacc ctaaacttat agttacattt atatactttg cttttaatat ctactatttt
     8401 aaaactattc tagtacctaa tcatattaga tatacaatgt tagtattagt atttttacgt
     8461 ttttagagtt ccacctacta tttatctcta acaatattac cctttacttt attgcttaca
     8521 gttataacct tcttattact gacatcaata gcatttcttg tagcttttct agctttacta
     8581 ttactatcag taagacttat tgtctttaac tcatcataat tcttagctcc tacaggtaca
     8641 tacattgtat cacttacaaa tctttgacct actggcgtat ctccttgaag gtcattgtat
     8701 ctatatagtt cagccttaac cttatcactt aaagaatcaa agttttcttt tggcatatag
     8761 taaacccatg tacctttact gatttcacct aattcacctt tttctggaac ttctgcaaat
     8821 aaaagtgctt ctccagatat agtcttattg tttttatcta taagtagtgg taaattaaat
     8881 gctggatttc cactgtattc acctgtaata attattgaac ctgctggaat cttacctgtt
     8941 tcatcttcac ttgtgctagg gtcatcactt tgatatacat aatcttctgc taactttcca
     9001 attccattag tacgagtttg gtctgcatta ttcattgtgc caatttctat attatcacct
     9061 ggaggtccta ttatatctaa ttctgcaact tctatattag acttattgtt tgagattagt
     9121 tttacatatc tagcttggta tgtccaaagt ttatcatctt tattaaatgt aactctagct
     9181 tcattatctt ggtcttttcc tccaccaact aaatcttgtc catattcaaa agttccttga
     9241 cttacatctt cccagttctt tttatcatta cttacttgta tcttgtaatt agttaaagga
     9301 ttgtatgttg tgctagtatt ctttctaaag ttaaataatc cttttaatga gaacttccta
     9361 gtttctgcct ctggttttgt atattttagt cctactatag gtaatgtttc tttcatgtct
     9421 actattatat atggattttc ttctacattt atatcagggt ctttttgagg gtctttgttc
     9481 cactctgctt tacttattat ctttccttta tatgtagtac ttacatcatt atcttttata
     9541 ttatcaattt caggattttg taatggtcca tgtgagtcat tttcagttct aacatcaccg
     9601 tcagaaacag tatttgttat gaatgaccat gctgatttat ttattttccc ttcatctgat
     9661 aattttacag tgccaacttc aaattcttct gttggattta ggtgataatc atatgcaact
     9721 accttatatg taaatgctct gttattaaga ggagatatac tatcagtaaa tttggcctta
     9781 ctatttcctt ttgcatccac aaatccaact gttctgtatt ttacctgtgt tccattttca
     9841 ccatctaaat aatttccatc acttcttatt atttcgtatc ctagtatctt atctttatcc
     9901 ttatctacat ctaggtctat agatatttta ttagatttaa ctagagaacc actttgtatt
     9961 ccatcagaaa aactagcact tactttagta tcttttgcca tactagctat attttcattt
    10021 aaaatctttc ttctagcttc gtcatttaca tattgtatct ttcttgattc tttttcatag
    10081 ttcttactat ccattatact gtttaattct ggtgtaattt ctactcccca tgccttaaag
    10141 taatctctta aatctttttc aacaacactt gatgccattg ctactagcat ttggtctttt
    10201 ggtaaactat taattgggtc actacttgaa agtgctctgt attttcttga aatctttgca
    10261 tagaatgtat cattacttaa atcagcatct gagttattct ttaacattga acccgtaaaa
    10321 tcatcatcat aagctagatg taattgccat aacattccaa gcctttcaaa tacattgctt
    10381 ggtataccta agctatttga agttacatgc tcatatatct tatccattgt tgaaccttca
    10441 agtcttgaat gtgtcttatc atcaaaggtc tgagcaagta aagcaagtac attgttacta
    10501 gtttcgctat atgtcatctt tccaatatct gtaacatgtc ctatttcatg acctattccc
    10561 catccaaata agtttcctcc tgtagctttt ccactatcat ctatttggaa aggtactcca
    10621 ttcataagcc ctggaactga accatattct actcctacat gtaatccaga agcatacata
    10681 aatgctccaa taaacattct ttggtatttt atgttcatac gagattttgg agccatatca
    10741 tttttagtta agttaaattc attatcatct acagttccat taccatctaa atcttgtgtt
    10801 ttaaatactc ctctttctga gtaaactaaa tccattattt gttcccatgc aagcatacta
    10861 ttatataatc tatctacttg tgtatttaaa tcagcatttc cttttgatat tccttgatat
    10921 acagctgttg caggaacatt taatgttact ttatcacttt caatatctgt agtgtttaac
    10981 acaccagttt tttctgaata ttcatatgaa ttaccaatat ttgttcctgg ataatatggt
    11041 tctcctttta cagcatcttt atatttagct aattcttcta tatatgcttt tatctttttc
    11101 ttagattcag aaactcccgc aggactctta tcatttattg ttccatttag attaagatgt
    11161 ggtatcttag tagctcctgc tacacgtact tttatatctc ctccacttgg agtatcttca
    11221 gggtatctta tgtataaact tcctccacat tctatatcta aactatgaag tttgcttaat
    11281 tgaattacat tttttccagt tttaagattt atttctccac tcttatatgc accagattct
    11341 ccatagtact gtgtatatat caacttagga gtttttccac ttttacttcc aacatatact
    11401 gttatttcat ctcctgctct agcagctact cctaatgcct gccagtcatt ttgcatgcct
    11461 aagttattgg aatttacagt atactgtccg tttttatctt tatattttcc tgatttattt
    11521 atttcttgat ttacagtagt tacatcagta agtgtggctc tatcattata taagtcttga
    11581 gctaatttta attctctatc aataactgtc ttatatggat gatattctcc actagcttta
    11641 tctggagtat ttaatctatc ttttaattca gttatttttt cttgagttac atcttctttt
    11701 aattcaactt gtaggtcatc cataaataga tttcttacat catcttctat actgtcatac
    11761 tcataaaact taagttctga tatattagac ttatatgaat aagatgaaca aacacttact
    11821 tgtatcttag tagcagttat tggttctttt aatctaacca ttacatactt attattattt
    11881 acagggtctg tctttgtttc aaaaggaaca tcccccagat tgtgtaattg accattttca
    11941 tcccaatatc gtatctcaaa gtcatttatt gtcttatcag aaacatcaaa acgaggtgta
    12001 gccataaaag tatccatagt ataagattta tcaaaagtta ttattggtcc tctattcgtt
    12061 ccctcagctg cattatatgt tgggaatgtc caatgtgttg tgaagtctcc atctactaca
    12121 ttgaatttat caaatggatt tttatcacta tattcacttt tgtcatatcc tgctggataa
    12181 acaacatcta caatgtgatt tgttggtatt ccaaatccac cattctcctt aggagtatta
    12241 attaatctat agttaggtgt tgttggtgca tctatgcttt cagttgtacc tatatatgtt
    12301 ttagacattc cactagttcc caagtggtta gtagctgtca ttctaacttc atatgttgtg
    12361 ttatccttaa gaccttctat ttttaaactt gtcccacgaa taagcttatc attgtatttt
    12421 atccaagacc cattagagcc tttttcttta tagtacaagt caaagtcttt agcttgttta
    12481 tgatttttcc agcttacatc aagagattta aatttacctt taacatttat accttctggt
    12541 ggttctggtt ttgtgtctgg aacaacttga aattctaata tgctatctgg gctaaattgt
    12601 ataggtttat agtttgagtc tacattttct ggtactccat ctaagttttc tggcgttgca
    12661 tctttataac cactagacca atctccattt aaagattgta tgctaaccct atatagtcca
    12721 tatgcttcta tatctctaac ttctattgta tttttactag tttgtaattt tttagtagat
    12781 aatacttttc ccttactatc aattttatct agtataagtt cgtatgctga tacatttact
    12841 tcattattcc aataaaaaga tactttttca tttccaacag cagtgctagt cgatatttta
    12901 tttattttag gtatgttcat ctctggcttt ggtatttctt tatatacatt gtttaaaaat
    12961 tcaacttttg atatttctgc aagatttcta tcgtttgctg ttgtagctgt tatttttata
    13021 gttatctttt ttactgctac ttgtttacca agatttatta ctatatcgct attttgaaca
    13081 tctgtatatg tttctaatgc tgccctacta tatgtagatg ttgtagagtt atcatcttta
    13141 ctgatattat ccccttcttt tggagtatct tcatctgaat taggtttatc cattgatgaa
    13201 ttttctttat cattattttt atcttgattg gtattagagt ttacttcttg tttatcgcta
    13261 ttttcctgtt gtttatcatt attttcatct ttattatttg aattattatc agtctgattt
    13321 ttattttcat ctttattatt tacttcttct tgatttactt cttcttgttc gttatttact
    13381 tcctcttgtt gctccttatc ttttaagttt ttcatatatt ctttattttc ataaggaatc
    13441 tgttgaacat taccatcttc gtcagtaacc tcaactattc cttttgctgg agcagtacct
    13501 tctggagctt ttatattgat gacttccata ttaagtgctt ctgataaagc tatatccatt
    13561 actgctggtt tttcctcaga tatttcatca ttttttgaag atattgtaac tccattattt
    13621 tctccactaa ataaattttc tacattacca ttaatatcta catcactatt tccttttaat
    13681 tcaaccttac ttgtatctac tataggattg gtagatacta tactttctag gctttttgca
    13741 tcttcacttt gtcctaaatt tttctgtaca taatctaagt caacaatatc tacctttcca
    13801 tctctattta aatcaaattc tcttttattt gaatctatat tctctaaaac cttattataa
    13861 tcactcttat ctactttacc gtctccattt acatcaccca ttaagaataa ttcataattt
    13921 aaaggtttat ttttattatc tacacttgct ctattgctta ataagactct ttgtgaataa
    13981 cttgatatat caatgtcttt aatttctttg ttcttaaatc cagctccaga gacttctata
    14041 ttgtattttc caacaggtaa attattaaat acaatattat aatatgatac attctcatct
    14101 ttatttttta tttcacttct acttgcagat aattttttta tagtatattc tatattatct
    14161 gtcccaatat ttactgttcc tgaatctttt tctccaagct ttattattcc actattgtta
    14221 ctatcatctt ttaatcgtaa ctgcatatta gtcatacttt ctgactcgct attttttatt
    14281 ggcagtgaaa aatttaaatc tacttcaaga ttccccctta acttttctgt aactgatttt
    14341 tcaactgccc ttagcttccc ctctaatata ggttctgcaa aaacatttag tggtgcacta
    14401 cacatagata ctgctagtac acaagatatt gcctttttca tgaaacaaca atcctccctt
    14461 aataaatctc taatttttca tgaatttaaa tactataagt ttcagacaat aaaaaaagac
    14521 actcaaagag aatcttaatt ataaaataaa aagactttac tctttttagc aactggctga
    14581 tatggcttta aaagctttaa tccctatagt tttgcgtcat tagatttccc taattttgcc
    14641 atttaatttt cactactttc ttacccattt tatactatat ttaaactttt taatacaatt
    14701 tttttacatt aaattataaa aaaataacat ttatattaat aaattaatat aaatgttatt
    14761 ttccattata atttaattat ttatatagct tccttacaag cataattttg tgtatatttt
    14821 cttattcttt atatcttcca taaaattcat gtgtagcata tagattctta tctcctgtta
    14881 tagctatacc aaatcccatt atttcatacc ctttatgcaa catattagtt ccttcttctg
    14941 gatttgtctt ccactcatta aaaataaagt ttcctatatc atctgcatct ctatttgtat
    15001 atacaggttt atctgaaatt ttatatttaa ttatattttc accttgaact gctatatatc
    15061 caggtttaat ctcactccaa tctaagtatt tcaaaaaagt agaatatgaa ttttttccat
    15121 ttatcgtatg agataaagtc ttcttatttg ccattaaatt agaccaatca tttgctagac
    15181 cctctaatct gcttgctaca gataacgctc ttactccatt atcttttcta tactgctttg
    15241 taagagcata tactttaccc tttattctct ctttaaatgc tctatcattt ggattgtaat
    15301 ataacccttg ttgatttggt gaagcttgat ttatacattg cttgataaca ttctgattta
    15361 tgcctcctag tacagtcaat ttgtctgcac cacttaaaat actcttgtta ctgccatttt
    15421 caactaaaac tacaggagca ttttttgcta ttgtagaggc gaccaaagcg tctgttaatt
    15481 gcaagccttt acttagataa aattctttac tatttttata aaactcttgt ataactatct
    15541 tattagtttc aaatctatct gtgccaccca atctaataga atttgtgctc ttaactaatt
    15601 cgtcgctgat tatttcacta gaaccaagcg cataactctt taagcctgtc gtgtcaaatc
    15661 ccacactatt tccatctata agtattatag gtactccatc tctagcagct actggtgatg
    15721 cacttatcgc atctgcttct cctgtaaatc cattggctat aattacttta tctattggct
    15781 taatttcttt gattttttca gctattttta tacttgtatc aaatctattt tcaccaaata
    15841 ttctttgtac aaccttcttt ttggataata actgattctc aacattttta cttattgttg
    15901 attctgagcc tattatatac acagtctttg ctttgtttaa tctttccata gttgtgtttg
    15961 gaatctgatt ttgctttgtc attaaaatag gcgcatttaa ggctccagct aaaccacttg
    16021 cacttagacc atctgctaag ctattatctg agttgattag tattgctgta ttgtagtttt
    16081 gcttatctgc tatttttgct gctgtctcat atctgtcatt cccttttatt tgctcaactg
    16141 atgtcaacgc atttaccata ggagtattta ccattactaa aaatagtgta agtcctatag
    16201 ctaatatctt tttactattc ttcatattat tttctacccc caatgtactt ttcatatttt
    16261 ttattagtac tcatacttat ttttaatcaa aagttacaat tttgttacca tattttatat
    16321 atgataccat agttatccat acaactcaaa caatatacct aaagttttta
//
LOCUS       DAGQWR010000095        16211 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.095, whole genome shotgun sequence.
ACCESSION   DAGQWR010000095 DAGQWR010000000
VERSION     DAGQWR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16211
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.095"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            80..658
                     /locus_tag="MIW95_002643"
     CDS             80..658
                     /locus_tag="MIW95_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBY3151958.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKFVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            688..834
                     /locus_tag="MIW95_002644"
     CDS             688..834
                     /locus_tag="MIW95_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBY3151959.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            1139..2518
                     /gene="scfB"
                     /locus_tag="MIW95_002645"
     CDS             1139..2518
                     /gene="scfB"
                     /locus_tag="MIW95_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBY3151960.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            2547..3044
                     /locus_tag="MIW95_002646"
     CDS             2547..3044
                     /locus_tag="MIW95_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBY3151961.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            3342..5006
                     /locus_tag="MIW95_002647"
     CDS             3342..5006
                     /locus_tag="MIW95_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBY3151962.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            5019..7469
                     /gene="recJ"
                     /locus_tag="MIW95_002648"
     CDS             5019..7469
                     /gene="recJ"
                     /locus_tag="MIW95_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBY3151963.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            7482..7994
                     /locus_tag="MIW95_002649"
     CDS             7482..7994
                     /locus_tag="MIW95_002649"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBY3151964.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            8068..10275
                     /locus_tag="MIW95_002650"
     CDS             8068..10275
                     /locus_tag="MIW95_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBY3151965.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            10300..10749
                     /locus_tag="MIW95_002651"
     CDS             10300..10749
                     /locus_tag="MIW95_002651"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBY3151966.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNASRPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            10764..11378
                     /locus_tag="MIW95_002652"
     CDS             10764..11378
                     /locus_tag="MIW95_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY3151967.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVSDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            11469..13025
                     /locus_tag="MIW95_002653"
     CDS             11469..13025
                     /locus_tag="MIW95_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBY3151968.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNRLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDKKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEELNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            13015..14277
                     /locus_tag="MIW95_002654"
     CDS             13015..14277
                     /locus_tag="MIW95_002654"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBY3151969.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            14351..16138
                     /gene="aspS"
                     /locus_tag="MIW95_002655"
     CDS             14351..16138
                     /gene="aspS"
                     /locus_tag="MIW95_002655"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBY3151970.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTKMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDIQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         6521 a         1636 c         3046 g         5008 t
ORIGIN      
        1 caactagttt tgtaaagtat attgaatttt ctcaaaaaag aatgtactct atatatgtaa
       61 aatttttttg gaggtaaaaa tgtttaaaag atatgctgaa aaaatgacat ctgttttaat
      121 ttgtaataat atgatagata ataatgaatc taaagtctat tcttatggat ttgaaatatt
      181 aatagctttc atagttaata taaccaccat gctgtttata ggttttttat ttggtaaatt
      241 tacatatgta ttattttttt taatgtgtta ttgtccaata agacaatttt caggagggta
      301 tcatgcagac aattatttta gatgcttatt aacctttatt tttataattt tatctacaat
      361 cttaattata gaaaatataa atatagactt atttaaaaat attattatga ttattgcaag
      421 tgttagttgg gttggaattt gtgttctgtg tccaatagaa cataggagta accctataag
      481 tgatagggaa aagtttgttt ataagaaaac tgctatattt atatctacag tagtgttatt
      541 gataacaatt ttaagtcttt ctttgagtat atttgtagat tatttcacat atagcgcatt
      601 tgccatgttt tggatttttg tgatgttagt attaggtaag ttaaaagcta aagtataagt
      661 aaaatatgta tgagggagag ttaagttatg aaaaagttta ttgtaagatt tatgaagttt
      721 gctagctcat tggcacttag cactgctatt ttgtcagcta atagtacctg tccttggatt
      781 attcatcagc ctaaagtacc caaagaaatc agcaatctaa agaagacaaa ttaaatacaa
      841 gcacttattt atacaaaatt atcaggaaaa aagaaattat tatattttaa taagattaaa
      901 acaatataaa atcagataca aaatttattt tatttatttt ataggagcct aatacctatt
      961 tggtaatata ttagtgcgcc aatttagggg gatatagtgt atttaatgaa atgacttacg
     1021 gagagtaagt catttttgta ttgtatgtat tttatgtaaa cttatgatat aatgaagggt
     1081 aaagtttaat taaaataata aaaaacttta gtatagatag taatttagga ggtagggtat
     1141 gagtatgata cataaatttt ctatgaatgg atataatatc gttttagatg tgaatggtgg
     1201 agctgttcat gtgttagatg atgttgcata cgatttgtta gatttttata aagaaaaaag
     1261 taaagaagaa atattagaga tattaaaatc aaaatatcaa gaagaaaaaa taaatgaagc
     1321 atatgaagaa atattaaatt tggaaaaaga aggtctttta tacacagaag atacatatca
     1381 atatcatcca agctttgtac atagagagcc tgtagtaaaa gctttatgtt taaatgtagc
     1441 tcatgattgt aacttaaaat gtaaatactg ttttgcagct caaggtgatt ttggaggaga
     1501 aaaagaactt atgagttttg aagttggtaa ggcagcaatt gactatttga ttgctaattc
     1561 aggaagtaga aagaacctag agatagattt ctttggtgga gagcctttga tgaactttga
     1621 agttgtaaaa caactggttg actatggaag aagtgtagaa aaagattata ataaaaatat
     1681 aagatttaca ataactacaa atggtgtact gttaaatgat gagattatag attatataaa
     1741 tgaaaatatg cataatgtag ttttaagttt agatggaaga aaagaagtta atgataatat
     1801 gagaccaact ttaaatgata aaggaagtta tgatattaca ttaccacgat ttaagaaact
     1861 tgtggaaaaa agagcaaaag ataagtatta ttatataaga ggtactttta caagagataa
     1921 tttagatttt tcaaaagatg ttatgcattt tgcagattta ggatttaaat taacctctgt
     1981 agagccagtc gttggagatg agagtaatcc atatgcactt agagaagaag atttacctaa
     2041 gatatttgaa gagtacgaaa aatttgctgt agagtatgca gatagacaat tacaaggaga
     2101 tggatttaaa tttttccatt ttatgataga cctaaatcaa ggtccatgtg taataaagag
     2161 aataactgga tgtggagctg gaaatgagta tctttctgta actccaaatg gagatatcta
     2221 tccatgtcat caatttgttg gaaatgaaga atttaaaatg gcaaatatat ttgatgaaga
     2281 aatagtactt ccagaaaatt taaaaaatat gtttagagaa gctcatgtat atactaaaga
     2341 agagtgtaaa caatgttgga ataagtttta ctgttcaggt ggatgtcatg caaatgcgat
     2401 aaactttaat aatgacatat caaaaccata tgaattagga tgtgaaatgc aaagaaaacg
     2461 tacagagtgc tctataatga tacaagctaa gttaatgtta gaaggagcaa ccaactaaat
     2521 ttatatttta aaggagttgt ttatatatga ttagagatta tttagaagat aaacctttaa
     2581 ttgacgaaag tgtatttgtg gcaaaatcag cagatgtaat tggaaatgtt aaaataggca
     2641 aagattcaag tatatggtac aatgcagtag ttaggggtga tgaaggacca ataactattg
     2701 gagaaaacac taatatacaa gattgttcta tagtgcatgg agatacagaa actataattg
     2761 gtaacaatgt aacagttggg cataggtcta tagttcatgg ttgtaaaatt agtgataatg
     2821 tgttaattgg tatgggttca ataatattgg ataatgctga aataggagaa tatactctga
     2881 taggagcagg tacactaatt acatctaata agaagtttcc accaggtgtt ttgataatgg
     2941 gttctcctgg taaggttgtt agagagctta cagaagaaga taaaaaatat attgatgaat
     3001 cttatgaatg gtatttagag gcagcacaaa atcaaaaata ttagtttaaa gagtggttgt
     3061 ttcaaaacat atttaaatct gttttgaggc aaccactaat ttattttatt agattttgtt
     3121 gatattttag ttttcaaaaa gtatttgatt aaaccagata taaaatgtca aaaataaaaa
     3181 ttaactgagt taaataattt taataaaatt ggtagtatat gtgataaaat taaatattaa
     3241 agaggatata ggatattaga atatcaattg gtgaaataat gtcatatacg ataaattaag
     3301 gtgtaagttt ttgggtatac ctatcttagg agtgaaaaga tttgagaata agttatagaa
     3361 atttaaagcg atatagagaa attggatatg ttttaataaa gtatggtttt agttttattg
     3421 tagaaagact aaatatagaa ggaattgcct ataaaatacc actatttgac ccaccagaag
     3481 aaataaaaaa tatgacaact ggtgagagaa tgaaaagagt tttagaagaa ctaggaccta
     3541 catatataaa aataggacag atattgagta caagaaaaga cttgttagac caggatataa
     3601 ttgatgaaat atcaaaactc agagatgatg tagagaaatt tgattcaaat atagctattg
     3661 atatatttaa agaagaagtg ggtttaagta tagaagaaat attcttagaa tttaaagaag
     3721 aaccaatagc agcagcttca attggtcaag tttatgaagg tgtacttaaa acgggagaag
     3781 aagtaatcgt aaaaattcaa agaccaaata tagagaaaat aataaaatct gatttagaga
     3841 ttcttagaac tattgcaaat actttaaaag atttaaaaaa ggattttaat ttagatttag
     3901 tacagatgat tgaagaattt caaactcaat taatgaggga acttgattat acatttgaag
     3961 ccataaatgc aactaaattc tctaggatat ttaaaaatag tgatgaagta tatataccta
     4021 aagtatactc agaatataat actaagaaaa ttttagtaat ggagaaagtt aatggtacaa
     4081 agttaagtga tgtagaaaaa ataaggagac taggttataa cacaaaaact attgttgaaa
     4141 taggagttag gtcatttttt acacaggtat tatctcatgg attttttcat gcagacccac
     4201 atccaggtaa tatatttgta gttgctaaaa ataagatagc atatatagat tttggaatga
     4261 ttggaattat tgataataaa actttaaacc aattaaatga aattgcatta gcaggtgtag
     4321 aaaagaatgt tgacaagata atatatctat tgatagaaat ggatgcatta aatggagagg
     4381 ctgatattaa gggacttaga caggatttac tttatctaat acattattac tatgatattt
     4441 ctatagaaaa aataaatgta acagatatat taaatgaact atttaggttt tttagacagt
     4501 ataaaatagt catgcctgca caatttgtaa ctcttgcaaa gacagttata acattagaag
     4561 gaacaagtag aactttaaac acagattttt cttttggttc tatgggaaaa gagtttatga
     4621 aacatcatta taagagtaaa tttaacccta aaaatgttgt tttaagttca agacaaaatg
     4681 tagaagaaat attactggac ataaaaacta ttccaaagca attaaaagca attttaaaaa
     4741 atatagaaag aaataatata aaaatgcaaa ttgaagatgt taaaatgacg aggttagaaa
     4801 attgtataat agagctaaca tctcaaatat ctttgagttt agtgttggca tctattatag
     4861 taggttcttc tcttataatt gcatctccaa atatagaaaa taatatatgg ataaaattta
     4921 ctgcaatagc gggtttcttt atatccttta taataggcct ttgcttagtt ataagaagta
     4981 ttaggtcaaa atacaaaaaa gattaggggg aattaaattt gaaaaaatgg atattgaagt
     5041 ataaaggtaa gatagaggaa agtgaattta gcaaaaaact taatatttcc ccggaaatct
     5101 gccaaatttt aaaaaataga gggatatgta ctgaaaaaga ttcagaaata tttatgaatc
     5161 catctttaga ttatttaaga gacccttttt taatgaaaga tatgcaaaaa gctgttgata
     5221 gaataaagtt agcagttgag aagaacgaaa aaatttggat ttacggagat tatgatgttg
     5281 atggtgtatc atctacatcc atcttgtgtt tatattttaa gagtataggt tacaatgttg
     5341 attactatat tcctaataga ttagaagaag ggtatggtat aaatgaggat gctattaagc
     5401 ttataagctc taggggatgt gacttgatta taagtgttga ctgtggaatt acatcagtgt
     5461 ctgaggtcaa tacagcgaat gatttgggca tagatgttat aattacagac catcatgaat
     5521 gtcaaagtga aattccatca gcctttgcag taataaatcc aaaacaagaa gattgtaatt
     5581 atccatttga ttcactatgt ggatgtggtg tagcatttaa aatgattcaa gccttgactc
     5641 caaaagaaga atttaaaact agtatgtata attatttgga aatagtaaca ctagcaacta
     5701 tttgtgatat tgttccactt atagatgaaa atagaataat tgtaaaaaat ggtttaaaat
     5761 caatgaaaga aggcaaaaat attggattaa gggagcttat aaaagtttgt ggtgtagaaa
     5821 gtgataagat aggttcttct catataggtt ttgcaatagg tcctaggata aatgcttcag
     5881 gtcgattggg atattcttac ttaggagtag agctatttac tactcagtca caagaagagg
     5941 cagtagaaat agctagtata ttggaagaaa aaaataatga aagacagatg atagaagcta
     6001 agatgtatca tgaagctgaa gaaatgctaa agtctaatag cagatacaat gatgacaaag
     6061 tgttggtttt agccaaagag ggttggcaac atggaataat aggaattgta gcatcaaagt
     6121 taactgaaaa atactacaaa ccaacaattc ttctaggtat agaaaatggg gaggctactg
     6181 gttcagcgag gtcaataaaa ggatttaata tatttgaagc tctcataaag tgcaaagact
     6241 tgatgactaa gtttggtggt catgaacaag cagcagggtt atccttggat tcagacaatg
     6301 tagaaatttt agcaaatgaa ataaataaat ttgcagatta taatttaact gaagatgata
     6361 tgatagagaa tgtaaatgtc gagtttgaat tacaagaaaa tgtaataaat ttaaatcttg
     6421 tagaagaatt acataagtta gaaccctttg gattaaataa tcctaatcct agatttatag
     6481 ttagaaatta tattttaaaa gatttgaaag ttataggtaa gaatcaacaa catctgaaat
     6541 taagtataga aaaagagaaa agctatgaat gtataggatt taatatgagt catttaaaat
     6601 caatgtataa agtaggagac aaagtagatg tactattcca attagatgaa aataactata
     6661 tgggaaatag gaaagtacaa tttttactta aagatatacg attggctcga cctaaaagtg
     6721 caagtaatga caaactttca ttgaaattaa tgtcaaaaat aataccaaaa gatactcaaa
     6781 gcctgtataa tatatctgtg tctgattttg aattatttga tggaaataca gatataaata
     6841 tatttgatta ttttgaaaaa gatacactaa taatatctaa ttctataaat ggattttata
     6901 gagcaatgtc agatatatca cttatagatt tggattttaa cataaattac aatattattg
     6961 aagatgttag caaaaatact gataaattag agcttatatt ttctcctaat attgataaaa
     7021 tagacttaaa aagatataat aatattattc tttatgatta tttatacaat aaaggcgaat
     7081 attcgtatat atatgagaat aaaagagaag aaagtgaaat tataaaatat tataataaaa
     7141 cagacctatt atatttaaag aatgtagtta gtaatatagt tccatcaaga gatgaattta
     7201 taacaattta taaacaagca cttataaaaa aagaaattga tttggatatg gtaaatataa
     7261 gggaaacttt taatgtaatt ccattaaagt tttttacgat attaaatgta tttagagaat
     7321 taaatttact tgactttaat ttaaattatg aaaaaaattc tgtactcata agaatattgc
     7381 caaaaccaca gaaaaaatta gacttaaatg agagtttaat actcaataat ctaaaaaatt
     7441 tagaaaaaca atacaatagc agttattagg aggcgtttcc catggattta aaaaacttta
     7501 taaggaatat tgatgatttc ccaaaaccag gcatagattt taaagatgtg acaactctat
     7561 ttaaagatgg agatgcattt aaatatgcag tggattctat agttgaagaa ttgaaagata
     7621 aagatgtaga tttagtaata gggccagaag caagaggatt tttgatgggt acacctgtag
     7681 catatgcttt aggagtagga tttgttccaa ttagaaaacc tggaaagtta ccaggtgaag
     7741 ttgagagtta tgagtatgga ttagagtatg gtacagatac tttagagata cataaggatg
     7801 caattaaaaa gggtcaaaaa gtagcaatag tagatgattt attggctact ggtgggacta
     7861 tggaagccgc tgccaagctt gtagaaaaat taggtggaga agtagtttct atgcaatttt
     7921 tgattgagct aaaattctta aatgggagag aaaagttgtc taactatgat gttaattctc
     7981 ttataaaata ctagtacttt tataatagtt ggtataacac caactatttt catatatatt
     8041 aaaattttat acaaaatagg tggttatatg catgataaag aattacaaga aataattgaa
     8101 aaaataaaag tttatgcacc taatgcaaat tttgaaatgg ttgaaaaagc ctataatctt
     8161 gcaaaaaaag cgcatgaagg acaatataga aaatctgggg aaccatactt tattcatcca
     8221 ttagcagttg caaatatttt aatagagatg gaattagata ttgaaactat aacagcaggt
     8281 ttacttcatg atgttgtaga agatacagaa tatacctatg aagacataaa aaaagaattt
     8341 ggcaaagaag tagcggattt ggttgatgga gtaactaaac ttggtcaaat aaaatatcgt
     8401 tcgaaagaag aaactcaatc tgaaaattta agaaaaatgt ttttggcaat ggcgaaggat
     8461 ataagggtta tacttataaa acttgctgat agattgcaca acatgagaac attaaaatac
     8521 atgcctccag aaaaagcaaa gtataaagca aaagagacac ttgaaattta tggtggtata
     8581 gctcatagat taggaatttc caagataaag tgggaactag aagacagagc attaagattt
     8641 atggacccag aagggtatta tgatttagtt agtagagtat caatgaagag aagtcaaaga
     8701 gaggattata ttcaaggaat tgttgaactt cttaaagata agtttaaaga tttaagtata
     8761 ccatgtgatg tatatggtag accaaagcat ttttacagta tatatagaaa gatgcaaaaa
     8821 aaacataaga cttttgaaga gatttttgat ttaacagcag taaggatatt ggttgataat
     8881 gtaaaggatt gctatgcagt acttggtata gttcatacat tatggagacc tattccagga
     8941 agatttaaag attatatagc aatgccaaaa cccaatatgt atcaatcatt acacaccact
     9001 gttgtaggtc ctgatggaga acctttagag atacaaataa gaactcatga gatgcataat
     9061 atagctgaaa atggtatagc agcccattgg aaatataaag aaggtatctc tagcaaagag
     9121 tcaaaggttg aagaaaaact tcaatggttg agacagatga tggagtggga aaaagattta
     9181 aaagacccac aagagtttat ggatgcactt aaagaagatg tatttaatag tcaagtttat
     9241 gtatttacgc caaaaggaga cgtaattgaa ttaccagcag aatcaacacc tatagatttt
     9301 gcctatagag ttcatacaaa tgtaggtaat aaatgtgtag gagctaaaat tgatggaaga
     9361 atagttccta ttgattataa gcttcaaaat ggtaatattg tcgaggtact tacatcatct
     9421 aattctaatg gaccaagtag ggattggctt aatatagtga agaccccaaa tgcaaaaagt
     9481 agaattagac aatttttcaa gaaggaaaga agagaagaaa atgttgaccg tggaaataca
     9541 atattagaaa gagaatttaa gaagcatgat ataccacaaa aagaccctct tgttgaaaaa
     9601 tatatggcat tagttgctaa gaaatttaat cagccaagtg ttgaggactt aattgccact
     9661 gtaggttatg gcggtataat gtcatctcaa gtggtatcta aggtaaaaga cctatattta
     9721 aaagaggtta aaaaagctaa aaaggaacaa cagatacaag aagaagattt aaataaacac
     9781 aatataagtg aaacagaata cagtaaaaag agaaagaaaa acgcatcaca aggaattata
     9841 gtaaaagggc ttgacaatat cttagtaaga tttgctaagt gttgtaatcc tcttccagga
     9901 gatgaaattg taggatatat tactaagggt aggggagtag ccatacacag agctgattgc
     9961 ccaaatgcaa acaataatgg tgatttcttc caaaatagat tggtggaggt ttcatggagt
    10021 tcagcaggaa aaggtaagtt tgaagcggaa attcaaataa aagctattga cagaagagga
    10081 attgttaatg aaataactca tattgttgca atggataaac ttagtttaaa cggaataaat
    10141 gcaagaaaag gtaaggatag tattgtaagt ataaacttac ttgtagaggt taatgacaca
    10201 gaggaattaa aaattctaat gaaaaaatta aaagcaattc ctggtgtaga aagtatttat
    10261 agaatgacaa attagagtta aaataatagg gaggaaacta tgagagcagt agtgcaaaga
    10321 gtatcttcat ctaaagttac agtagatgaa aacactatag gtcaaataaa taaggggtta
    10381 ctagtattgt taggagtgac tcatgatgat aaatcaagtg atgtagatta tatgattgat
    10441 aaaatattga atctgagaat ttttgaagat gaaaatgaca agatgaattt atctctaatg
    10501 gatataggtg gagagctttt agtagtatct caatttactt tatatggaga ttgcagaaaa
    10561 ggaagaagac ctggatttag taatgcatct agaccagaac ttgcaaataa cctttatgaa
    10621 gaatttgtaa aaaaagcaaa agataaaggt gtgacagttg gtacaggtca atttgcagct
    10681 catatgatgg tagagttaac taatgatggt ccagtaacaa tactattaga ctcaagtaaa
    10741 tctttttaag ggggaaaact ttaatgatta taaaaaaatt tgtagatagt tattttggag
    10801 taaatactta tgtattaggt gatgaaaaga ctaaaaaatg tgcagttata gacccaggtg
    10861 gaagtttagt agagacatta agttttgtaa aagaaaatga attaaatatt gaatatatta
    10921 tccttactca tgggcatgga gaccatatag gatatgttaa ggatattaaa gaaaaaactg
    10981 gtgctaaagt agttgcacat gttgatgaaa aagaattatt aaatgataaa aataaaaatc
    11041 taagttatac aatgagatgt ggagcgcaag agtttgatgc agatatctat gtgagtgata
    11101 aagacaagct tgaacttggt gaattaaaaa tgactttctt gcatactcca ggacatactc
    11161 aaggatgtat gtgtataaga gttgaagatg aaatgttcac aggagatact ttgtttgctg
    11221 gaagtattgg tagaactgat ttatatagtg gagactttaa ccaaatggaa aaatcactta
    11281 agaaattatg caaatatgaa gatgctgtta gagtatatcc tggacatgga ccaaccagta
    11341 ctctaggggt tgaaaagaag acaaatcctt acatgtaatt ttatattagt atttaaaaaa
    11401 gtataaaata tttaataact atattagtga gggggttctt ctactgttaa atagaaggag
    11461 ttaagttgat gttgtatgtt tttttaaaag gacatgatta taagtatgaa gtagcagaac
    11521 ttataaaatt gtttaccagt gaatttaagt ttaaagactc taatgctaac aataatatag
    11581 agtcaaagca tttaataaat agattgattt atgagaatgg tgtattattc tcaagtactg
    11641 aatactatga aaatggaaat ttaaagtacg agtcaataca gaatatagaa aatatgaatc
    11701 ttgaattttt agatggattt gatagtagca aaacgattga agaatttctt aatagactag
    11761 atagtgaaag taagaggaac tttaaaaagc tgtgtaaaga aaatatcaaa aaaagcatgt
    11821 ttgtagtgct taaacaagta tttaattctt atgtaccatg gggaattttg actggtatta
    11881 gaccagtcaa aattgtccac aatttaatgg ataaaaagct agatgataat agcataagaa
    11941 ccattctaaa agataattat tttataatag ataagaaaat agatttagct cttgaaatag
    12001 ctaaaagaga gcgattattt atgtatccta tagataaaaa taaaatttct ctttatgtaa
    12061 gtattccatt ttgtccaact agatgtgtat attgttcatt tccttcaaat tcattaaaac
    12121 aatttggaca tttaaaaaga gaatatgttt ataaattgat tgaagaaatt aaggggtttg
    12181 ccaaagttat taaggagact aagaaagaag ttgaaactct atatattggt ggtggaactc
    12241 caactacact tgatgaggaa gaattagatt tgcttataaa ttcattgttc aatgaattag
    12301 atttaagtaa aattaaagaa tttactgtag aagcaggtag acctgacact attacagaac
    12361 aaaaactaag agtgttaaaa aaacacaatg ttagtagaat tagtataaat cctcaaacta
    12421 tgaatgatga taccttggta aagattggaa gagagcatag agtatctgat ttagtagatt
    12481 gctttaatat ggctcgtaga atgggatttg ataatataaa tatggatata attttaggtc
    12541 ttatagatga agaattaaat atggtaaaga acacattgga ggagataaaa aaactttctc
    12601 ctgaaagcct tacagttcat acattagcca taaagagagc atccaactta aatataaata
    12661 tggataggta taaagaacat ctaacacaat atgaagaaat ggtaaaaatg atagatttat
    12721 caatgaaata tgcaaaagat atgggattaa atccatatta tatgtacaga caaaaacata
    12781 tgttgggaaa tttagaaaat ataggttatg ctaaagaagg atatgagtgc atttataata
    12841 tacagattat ggaagaaaaa caaagtaact atgcattagg tgcaggtgct atatcgaaat
    12901 ttgtttatgt agatgaagat agaattgaaa gagtagaaaa tgtaaaaaat gttgaacaat
    12961 acattgaaag agtagatgaa atgataaaaa ggaagaaaga agaggtatat aaaaatgttg
    13021 actaaagctc ctagaggaac taaagacata actccaaaag aggcttacaa atggcgttat
    13081 gtagaaaata aatttagaga aatatgtgct ttatatggat atgaagaaat ggtaacacct
    13141 atatttgaac atacagaact tttcaagaga agtgttggag atactacaga tatagttcaa
    13201 aaagaaatgt attcttttaa agataaaggt gatagagaaa ttaccttaaa gccagaaggt
    13261 acagcaggtg tagtaagagc atttatagaa aataaattat atgcagatac tcaaccaaca
    13321 aaattatttt atgtgacacc ttgttttaga tatgaaagac cacaagctgg tagacaaaga
    13381 caatttcacc aatttggaat agaagcactt ggaagtgata ctccttcaat ggatgctgaa
    13441 ataatcgcac tagctgttca attttttaat gaagttggat taaatgattt ggtagtgagt
    13501 ataaattcag ttggctgtcc agtttgtaga aaagagtaca atgctttatt aaaagagtat
    13561 cttgactcaa aagctgatat attgtgtgat acttgcaatg agagaagaga aaaaaatcca
    13621 atgagggtta tagactgtaa aaatcctaca tgtaaagaaa atatcaaaga tataccattt
    13681 attgcagacc atttgtgtga tgattgtaaa tctcactttg ataaacttca agaatactta
    13741 aaggaaatga atataaactt tgtaattgat aaaacaatag ttagaggctt agattactat
    13801 agaaaaactg cttttgaaat tatatctaat gatataggag ctcaaagtac tgtatgtggt
    13861 ggaggaagat atgatggtct tgttgaacaa ttaggaggac caaaaggtat aagtggaatt
    13921 gggtttggac taggaattga aagactttta cttacacttg aaggtaatgg aattgaaata
    13981 gaaaaccctc aatctacgga tatatttata gtaactatag gtgaagaagc aaatacaaga
    14041 agctttaaat tattaaaaga cttgagacaa aatcatataa gtgcagataa agaccatata
    14101 gagagaagtg ttaaagcaca atttaaatat tcagataaaa taaattcaaa atttacaata
    14161 gtgattggag atgatgagct taaaaatgac actgcgacat taaaaaatat gaaaacatca
    14221 gagcaaacta ctgtaaaatt aagtacatta gttgaagaat taaagcaaaa gctgtaaaat
    14281 aagaaagtat gatgttttgt gtattagata aatatatagc attaaaatta aataaaatgg
    14341 aggtagtatc ttggaaactt taaagggttt aaagagaact cactactgtg gagagttgag
    14401 agaaaaaaac ataaatgaag aagtcgtact tatgggatgg gtacagaaaa aaagaaattt
    14461 aggtggtctt gtatttgttg atttaagaga tacaagtgga ttatgtcaaa tagtttttga
    14521 tacagatgta gataaagaag cttttgaaaa agctgaaaaa ttaggggctg aatttgtaat
    14581 agctgtaaaa ggaaaagtat gcgaaagaca atctaaaaac cctaatatgc caactggtga
    14641 tatagaaata tttgcaactg aacttagact tttaaacaag tcagaaactc cgcctattta
    14701 tataaaagat gatgatgatg tttctgaagc actaagatta aaatatagat acttagattt
    14761 aagaaaacca tctatgcaaa gaaatttaaa attaagacat aaagtaatga atattacaag
    14821 aaattactta tcaaacaata gattttgcga aatagaaaca ccatttttaa tagctccaac
    14881 accagaagga gcaagagatt atctagtacc aagtagagtt aatccaggaa aattttacgc
    14941 attaccacaa tcaccacaat tatataaaca attattgatg gtaagtggta tggataggta
    15001 tttccaaata gttaaatgct ttagagatga agatttaaga gcagatagac aaccagagtt
    15061 tacacaaata gactgtgaaa tgtcttttgt cgagcaagaa gatgttatga gtatgattga
    15121 aggtttgctt gaagcgatat ttaaagaagt tttagatgta gaattggcac ttccacttcc
    15181 taagatgact tatgcagaag ctatgtctaa atatggttca gataaaccag atactagatt
    15241 tggatatgag ttaacagata tatctgatgt tgtttgtaac tgtggattta aagtgtttgc
    15301 tgatgctaca caaccaggaa aaagtgttag aggtataaac gtaaaaggca aagctgacga
    15361 ctttactaga aaacaaatat catcactaga agaacatgct aagacatata gagcaaaagg
    15421 tcttgcttgg atgaaagtag gacaagaagg agtaacttct ccaatagcaa aattctttaa
    15481 tgaagaagaa atgaacgcta tacttacaaa aatgaatgct gaagtaggag atttactttt
    15541 atttgtagct gataaaaact caatagtatt tgatgcttta ggacaagtta gacttgaagt
    15601 agcaaatagg cttaatctat tggataaaaa tgtatataac ttactatggg taactgagtt
    15661 cccagttttt gaagaagatg aagaaacagg cacattttca gcaatgcatc acccatttac
    15721 atcacctatg gatgaagatt tagacaagtt agaagaaggt gataagtcat ctttaagagc
    15781 gaaagcttat gatattgtat tgaatggtta tgaaataggt ggaggaagtg ttagaatatc
    15841 aaattctgat attcaatcaa gaatgtttaa agcacttggt ttcacagagg aaagagctaa
    15901 tgaaaagttt ggttatttat tagaagcatt taaatatggt actcctcctc atgctggtct
    15961 agcttttggt cttgatagac ttgttatgtt acttgcagga actgataata taagagaagt
    16021 tattgcattc cctaaaaatc aaaatgctgt ttgccctatg acaaatgctc ctacattggc
    16081 ggaagatgag caattagaag aattaagtat aaaagttgat ataaaagata atgaataata
    16141 tgtagttgca agaaactaaa atgtaaaaag tctggatata aagtatattt atattcagac
    16201 tttttttatt a
//
LOCUS       DAGQWR010000096        16040 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.096, whole genome shotgun sequence.
ACCESSION   DAGQWR010000096 DAGQWR010000000
VERSION     DAGQWR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.096"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            216..2075
                     /locus_tag="MIW95_002656"
     CDS             216..2075
                     /locus_tag="MIW95_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY3151971.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVC
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICINKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEEILDKEFVYNS"
     gene            complement(2367..2966)
                     /locus_tag="MIW95_002657"
     CDS             complement(2367..2966)
                     /locus_tag="MIW95_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY3151972.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3069..3866)
                     /locus_tag="MIW95_002658"
     CDS             complement(3069..3866)
                     /locus_tag="MIW95_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBY3151973.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3979..4084)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4176..5162)
                     /locus_tag="MIW95_002659"
     CDS             complement(4176..5162)
                     /locus_tag="MIW95_002659"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBY3151974.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLNEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5646..7376
                     /locus_tag="MIW95_002660"
     CDS             5646..7376
                     /locus_tag="MIW95_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBY3151975.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7486..7566
                     /locus_tag="MIW95_002661"
     tRNA            7486..7566
                     /locus_tag="MIW95_002661"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7520..7522,aa:Leu,seq:tag)
     gene            7798..7971
                     /locus_tag="MIW95_002662"
                     /pseudo
     CDS             7798..7971
                     /locus_tag="MIW95_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     regulatory      8463..8567
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            8777..11251
                     /gene="gcvPA"
                     /locus_tag="MIW95_002663"
     CDS             8777..11251
                     /gene="gcvPA"
                     /locus_tag="MIW95_002663"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBY3151976.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHHETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11251..12708
                     /gene="gcvPB"
                     /locus_tag="MIW95_002664"
     CDS             11251..12708
                     /gene="gcvPB"
                     /locus_tag="MIW95_002664"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBY3151977.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPLIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            13131..15488
                     /locus_tag="MIW95_002665"
     CDS             13131..15488
                     /locus_tag="MIW95_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBY3151978.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKDDFLYSGSFVVSGEAFVRVENIGKDNYANKIVNDAKISKKHNSQLRNSLN
                     SILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKIRIEVEKYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDNDIYEAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(15574..15891)
                     /locus_tag="MIW95_002666"
     CDS             complement(15574..15891)
                     /locus_tag="MIW95_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBY3151979.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         6053 a         1873 c         2623 g         5491 t
ORIGIN      
        1 ataaattata ttaatagtaa taatctataa gaacttatat attagaaaaa aatttttgta
       61 tatataaagg actctaaaga tgaactatat aaaacaaggt ttatatattc taatagtata
      121 gaaaatttta ttgtaaacgc atgtatatta ttagaaaaaa tagtttgaag gtattcaata
      181 aacataaaaa acaatatagg ggataaaggt gttttatgaa gagatttttg aggagaatta
      241 tcttagtttt atttattttg ttgattttta taagttttat ttctattaaa ttaattcata
      301 atgttggaga ttatggaaaa ttgattaatt atgttgggat tgtttgtgga gctagccaac
      361 gtctaactaa actagagatg aatcatcaac caaatgatga attaatagaa tatattgatg
      421 aaatcctaca ggagttgatt acagggcatg gagattatgg tttagtaatc actgattgta
      481 atgaatacaa tgaggactta cttttattag aaaaaaagtg ggaagattta aatattgaaa
      541 ttaaaaaagt aagaatgaaa gaacagaata accagttatt aagtataagt gaagagtttt
      601 tttctcttgc aaatgataca gtgtttaaaa tagaaaactt ttctaaagaa aaaagtaatt
      661 acttaatgac tttaataata attatatcta ttataggtat attagcatgt atcatattga
      721 ttttacaata tagtaaaaaa atggttagac tggaaaaatt aaatgtagat ttaaaaaata
      781 ttgcatataa agatgaacta acaggagtaa atactataga aaaatttaaa ttggatgcaa
      841 atcaaaatat ttgtataaat aaggataaga aatttgctgt attctatatt gattttgaaa
      901 actttaaata cattaatgat atatttggat atgattatgg tgacatgata ctgaaaagat
      961 atgctaatct tatgatgaat gacataggga aatatgaaat atttgcaaga gaaattgcag
     1021 atagatttgt tgcgttaaga tgctatgtag ataaggaaga tttagtagta agacaacgag
     1081 atgtagacaa tgaactaatt aatataacaa atgaaattaa aaataaacat agtattactg
     1141 ttgtcagtgg aatttgttgc attgaagatg taaatgaaaa attaagtata gatggtttaa
     1201 taaatagagc aaattttgca caaaaaactg ttaagaataa accaggtact aattatgctt
     1261 tttataatga cagtattcgt aaaaaaatga ttgaagaaaa tactatcaag agtagaatac
     1321 atgaagctat tgaaaaaaga gaatttatag tttatttaca gcctaaagtt aatttacata
     1381 gtcaaaaaat aaattgtgca gaagctttag ttcgttggct tactccggat aaaggtatta
     1441 tatctccagc aatatttatt ccagtacttg agaagaattt ttttattgcc ttagtagata
     1501 agtatgtttt tgaggaggtt tgtaaatgga ttagaaagag gttagatgaa aataaacctt
     1561 ttgttcagat atcagttaat gtttcaagaa tacaatttta taacactaaa tttgtagaaa
     1621 cttattccaa tatacaaaat aaatacagga tacctaaaaa tactatagaa attgaattta
     1681 cagagagtgt tgcttttgag aatcaaaaac atcttttgga aattatacat gatttgcatg
     1741 aaaatggatt tacttgttct ttagatgatt ttggaaaagg ttattcgtct ttaagtgttt
     1801 taaaagattt accatttgat gttttaaagc ttgactctat gtttttcaag gctagtttag
     1861 ataaagataa agagaaaata gtaataaaaa atattgtaca tatgttaaaa gagcttaata
     1921 tcacaactgt tgctgagggc attgaatgca aggaacaagt tgaatttctt agggatattg
     1981 ggtgtgattt agttcaggga tttgtatttt ataaaccaat gccaatatta gaatttgaag
     2041 aaatattgga taaagagttt gtttataata gctaaaatgt actttataaa atatgaacta
     2101 attaagtaaa attattttgt actgaataaa tttttacttt atttacagaa tagtgattat
     2161 ttacagaaaa gggcgaaaag tgtattcctt taagtatggg atggatagca caaatagggg
     2221 agaaaaaata ttggccatat aagaatgcat aataatataa atatttgaaa ggtcagcatt
     2281 atataaaaat taaaaagaaa ataaataatt tttctataaa aaagagacta tgagaaaagt
     2341 tactcatagt ctcctaaata atgtatttat tttcttcctg caacccattt taagccccaa
     2401 tgaaattcag tatccatttc tctatgacct ttgaagtatt taactacttt ttcaacactg
     2461 aatgtctcat catttacatt ttctatcaat gctattgaac acatattata gccattttta
     2521 tgttcatcaa gtttgacaac taagtctgaa tcttgttttt gtttgattgt tacaattcca
     2581 tcagcttcag accagttagc tacaccttcg tatataaaag cataaataag tattcttttt
     2641 atatctttta ttttattacc atttattctt aaattttctc cttgtgtatt acttcctgtt
     2701 ctgtcatctc catctaattg agcaaaaggt ggattgtcta aactaccaaa agcatttcct
     2761 aatgcttgaa cagcaccttt tacaccattt ttcatctcaa ataggcaacc caaatctaaa
     2821 tctatattac tgcttcttaa agagccaaaa aaaccctttt tttgtacttt ttgattccaa
     2881 tttaaattaa ctaagatttc acctaaatcg ctattttcct tttttgtaag gtttattttt
     2941 tgtccctttt ttaattctat agccatagta atcttcctcc acaaacatag atttatttaa
     3001 atggtagcac ttaataagtc aatattcaac aagattagaa taataatatt aaaactatac
     3061 taaatatact aaaaagtagg aatcacaata cctttgtatt tttcttcaat taagcttctt
     3121 gctttatctg atgtaagaac atcttgtaga actttaatct tttcactgtc tttatcttca
     3181 gttctaaatg ctaaaagatt tgcatactcg tcatttatag gagctgtata aattccgttt
     3241 ttcttggcat caagaccagc aggtacagca taagtagtat ctataaatgc agcagtaaca
     3301 tctggaagag aagatggaat ttgagcaata tctaactctt tgaattgtat tccctttgga
     3361 ttttctatta tgtccttagg actaactaat ccatcccctt taggtaacgt taaaagtcca
     3421 atactttcaa ggtatctaag gttcttagag caagaagatg ggttattgct tattgcaatt
     3481 gtatctcctt ttttaaattc atcaacagat tttatcttat aagagtaaag tattgctggg
     3541 catacatata gttttttacc agccattatt ttgtaacctt tggccttaac agcttctttt
     3601 agataaggtt catgttgaaa tagatttgca tcaatttcac cattatttaa agcttcgttt
     3661 ggaagtatgt aatcatcaaa gtttttaact tctaaagtat atcctttttc tttaataagt
     3721 ggttttaatt cttctaataa ctctcctcct ggaactaaag tagcaccaac aacaattttt
     3781 ttatcatctt tagctttaga acatccaacc acagctaaag ttagtactag acataataac
     3841 aaacataata attttttgaa tttcatataa attccctcct gaatataatt gattagttaa
     3901 aaataaaata ccattcttta tcacactgca aaataaaaat aagcctgagt ttaaactcag
     3961 gctttaaaag ctaatttaga ctcatctttc agtataatac tgcaggattt agcaccaatg
     4021 tatataaaaa tatactggtt gctgggtttc atagggccag tcccttcacc actctggata
     4081 agaagtatca atttttaatt atgttataaa tattatcatg attttttacg tctgtaaaca
     4141 taattttata aatattttaa aatctttaat gtattttatt taacacctaa aattccagaa
     4201 actagaatat cagtatcagc acctaagaaa tagtcattta tttcaataga atccagagta
     4261 tttctcaaag cattttcttc atgccttata ccaataagtt tattttctac aaatgcaaca
     4321 tcacattttc caaaaaaatc tccaaaaaat tttatactag ttattatgcc tttatcaaca
     4381 tttaaattaa actcaacatt tccaccagaa tattttaatt catttgaaag agtatatttt
     4441 ggtgaattac caaaattcca atcccaagtt tcgtattttg atttagtaag cttttctatt
     4501 tgttttatat catgctcatt caaacaataa ttagtattat cagtatttgt tttagaaaga
     4561 taatccatta tagcatcttt aaattctaat atgtccattt ttgaatgtaa atgttcactt
     4621 atattggtta cacgagattt tacagatttt attccttttc cttcaaattt tataggttta
     4681 acctttaatg cttttgagag gtcatttatt tcagatgaaa ataaaagagt accatgatgc
     4741 attactttgt ttttgtagtt gtattgagca tttccagaaa atttcttgcc atctataaga
     4801 aggtcatttc ttccagaaaa ttcggcattg acatctagtg ttttaagtaa atctattatg
     4861 ggctgagtga atcttctgaa atcactaaaa ttattattgt tacaagctat aaatgtaaaa
     4921 tttatatttc ccaaatcatg aaatacagct ccaccaccag actgtctcct aacgacaggt
     4981 atagaatatt cttttatata ttctaaattt atttcagaaa gtgtgttttg gttttttcca
     5041 acaattatag aaggttcatt cctccataaa ataaataagt cttcatcaaa attttttaga
     5101 agatattctt ccattgctaa attaaaataa gggtttgttt tttcattata aattaataac
     5161 attaaagtag ttcctccata gaattgataa aatttttatt atgaatacaa ttaattttat
     5221 aattcgacaa taatataaaa actcctttaa attagaaatt ttaaaaacta ccttgcaaag
     5281 tttaaataaa taatatatac tcaaaatatg tttaataaaa gtgaatagaa gggtagaggc
     5341 gcaataatct atcagtacta ataaggagtt aagcacagag agttattagg aaaggagata
     5401 ttgccgaagt tttataatcg atgctttaag attatattgc tgggggtaca tagaaaatat
     5461 gtacaactgt cacaggttta tttgtggtga gctatcattt aaggaattat attatattta
     5521 tttttactat tgttgatata ttgtaatgtt taagccttga gtgtttacca ctcaaggctt
     5581 ttttagtttt attgtaacat aataccctgt acagttcaca aaattaaaag gagagggaga
     5641 tttatatgag gaacaaaaaa attaatatta tttttctgac gacaatcatg ttcataatgt
     5701 caacagtaat ggtttttgca gaagaagata tagacactat tgcactagct aatgcagaaa
     5761 aatttgggat tttaacttta ataccaccat tagttgctat aatacttgca tttataacta
     5821 aaaacgtaat aatttcttta cttataggta ttctttctgg tagttttata attaaagcta
     5881 gtggtattaa tgtttttgcg acatttatac aggcattttt agatttagta gatagagctt
     5941 tagtatcttt agcagaccct tggaatgcag ggataatact acaggttttg gcaattggag
     6001 gagtaataaa tcttgttgct aaaatgggag gagctaaagc tatagctgaa gcacttgcta
     6061 aaagagcaaa gtcagcaaaa gggacacagc ttataacatg gtttttgggg cttttagtat
     6121 tctttgatga ttatgccaat tcactaatag taggtcctat gatgagacct gttgcagata
     6181 aaatgaaaat atcaagagaa aagctagcat ttataataga tgccacagca gcaccagttg
     6241 ctggtttagc tataatatct acatggatag gtcttgaggt tggtttaata catgatgctt
     6301 ttgaaagtat aagtatagat gtagatgcat ttggtatatt tttaaataca ataccattta
     6361 gattttacaa tatattaata ttagctttca ttgtaatatc agcattatta ttaaaagaat
     6421 ttggtccaat gagaaaagct gagataaaat ctagaagtag aaaaattagt atagaccttg
     6481 atgaaggcgt agaagagttg gatgatttag ctccaaaaaa tggagtaaaa ttgagtgtat
     6541 ggaatgctat aatcccaata ggaacattga taatagttgc attagcatca ttttattata
     6601 gtggatatac atctataatg ggaggagacg ataaggctct gatacaacta tttacaaatt
     6661 ctccatattc ttttgaagca ataaaagaag catttagtgc atctgatgca tctagagcat
     6721 tatttcagtc tgcacttgtt gcaagtttag ttgctattat aatggctgta gtaaaaaaga
     6781 tttttacaat atcagaagca attgatgttt ggatagatgg aatgaaaagt ttagtaatca
     6841 ctggtgtaat attaatactt gcatggtcat taagttcagt aataaaagag ttaggtacag
     6901 ctaagttttt aatacaccta ttatcaggtt cactaccacc attcttatta ccaagtttaa
     6961 tatttggttt aggagcaata atatcttttg ctactggaac agcttatgga acaatgggta
     7021 tacttatgcc acttgcaatt ccattagcat actcattaaa tcctgatatg tcttatgtaa
     7081 tagtaagtac tagtgcagtt ttaacaggtg caatatttgg agaccattgt tctccaattt
     7141 cagatacaac aatactttct tcaatgggag ctggctgtaa tcatatagac catgttaaca
     7201 ctcaaatgcc atatgctata tttacagcag taatcactat tgtatttggg tatataccag
     7261 ctggacttgg attgccaata tatatagtat taccagtggc aatagctgct atatttgtag
     7321 gtattcaaat aattggtaag aaagttgatg aagcggaaat agaacttgta gaataaagat
     7381 ataaataagt ttcacctttt attatcacta ttttgttagt ttttagataa ttttaaaaag
     7441 ttattgaaaa taatggcgaa atatagtaaa ataaaagagt gataagcgga tgtggcggaa
     7501 ttggcagacg cactagactt aggatctagc gccattggcg tgggggttcg actcccttca
     7561 tccgcacttt acagtactta attgtactga aaaaaactgg ggttgctggc tttcggagtg
     7621 ggctatcccg tttttatttt tttgtatata taatagattg gcactttagt ataaggtgct
     7681 ttttttatag gcaaaaacag tacatttatt tctgttttat tatcattcat ctattagaat
     7741 atactttatt taagaatgtg ttaataagca gtgttcacat tcttgataaa atgtggaata
     7801 atatgcctac caataagcat ttggtgtata agtaagagta tttatatagt tgattgtgaa
     7861 atgagtacaa caattctgta cagtataatt ggcattataa ttgtagcatt gaatttaaag
     7921 tttgcacagt cgctgattgg aaaatttaca aagtggatgt caaacatata aattttagtt
     7981 tattggacag ttcaaggaat cagaaattgg aattttatag tattaatatt ttatagtatt
     8041 aatataatgt ataaaatttt atagcaatgt atttcataag tataaagtga ctagaatttt
     8101 aagaaatata caaattctat aaaaactaaa taatttcaag tatattttaa aaatcttatt
     8161 tttcacaagt ttaaaattgt aaacgatttc atcaagtcta tgaatatact tttaacatgt
     8221 tatagtaaat ctcaatataa atttacattt ggttctttgt gtgttatttt aataataaat
     8281 taagtgaaaa tgttatttta cattaatata taatatcttt gcataagagt gaataatata
     8341 aaagagaaag aattgataat aaaaacttaa caaaaaaatt aatacataaa aaaaaataaa
     8401 tataatgtgt tgacatttaa ttgaaatatt catacaataa aattgaaaat aaatatttcc
     8461 aaatgagatt agcgagagag tcctttatac gaaaaggcgc cgaagaagaa atttaagaaa
     8521 tgccaatttt tagagaagct ttcaggcaaa aatatcgcta attgatggga ctctggagag
     8581 acttaagtta agatactata agtcaccgaa gaagcaaaat aatattaaat tattaaatct
     8641 ttcaggtata caaacaggga tatataggct aggattattt tccatgctga tatatctttt
     8701 ttttatgaaa aagtacagga aataatatca aagttactta aatacagtag gtctaatttt
     8761 ctagggaggg ttatatatga atgaattgaa aagagtatct ttgtacaata tacacaaaga
     8821 gcttggagca aaacttgttg aatttgctgg ctgggaaatg cctttagaat atgagggtat
     8881 aaataaagag catgagaagg ttagaaaaag tgcaggtata tttgatgtat ctcatatggg
     8941 agaggtacag ataaagggag cagagtcaga aaaatttata caaaacctag ttacaaatga
     9001 tataagcaca ttaaagataa atgacattat atatacacct atgtgttatg aaaatggtgg
     9061 agtagttgat gatttattaa tatataaatt tggtgaagaa gattatttat tagtaataaa
     9121 tgcaggtaat atagataaag atgtagcatg gataataaaa caaagtgaag gatacaatgt
     9181 agatataaaa aatatatcta gtgaagtgag tcaattagca attcaaggac ctaaagcaga
     9241 agaaattctt caaaaaatca cagatataga cttaaactca ataaaatttt ataagtcaat
     9301 tccatcaaca aaagtatgtg gttgtccttg tttagtttca agaacaggat atactgggga
     9361 agatgggttt gagatatact gtaaaaataa atatgtagaa ataatttgga atgaagttct
     9421 aaaggttgga ggagaagata tatgtccagc tggtctaggt tgtagggata ctttgagatt
     9481 tgaagcagct ctacctctat atggtcatga aataaatgaa cacatatctc caattgaagg
     9541 tggtttatct atatttgtga aaacaaataa agaatcattt attggtaaat caattttatc
     9601 aaaagaaaaa gaaagtggag ctaaaagaaa gttagtagga tttgaaatgc aaggtaaagg
     9661 aatgcctaga aatggatatg atataaggat tggtgataaa acagttggat ttgtaacaac
     9721 aggatgtgca tctccaacga caggaaaaat tttaggtatg ggaattatag attctgaata
     9781 tgcaaaagta ggaaatgaaa taggtatagc aatcagaaaa aaagttgttc ctgctgtgat
     9841 agttaagaaa cctttttata aaaaacaata taaaaaagat aatattatat taaacaaaga
     9901 aaataaattt tcatatatac ctgctacaag tgaagataaa tcaaaaatgt taaaagtagt
     9961 tggattaaat tcggttgatg aattattttc agatatacca gaagaagtaa aattaaaaag
    10021 agatttaaat ttggaaattg gaaaatctga attagaggta agcaaaatag taaaaagatt
    10081 gtcagaagaa aacttaagtt tagaagactt gacttgtttt cttggtgctg gtgcatatga
    10141 ccattatata ccatcaatta taaaacatat aacttcaaga tctgaatttt atacagcata
    10201 cactccatat caagctgaaa taagtcaggg aactttacaa gtagtttttg aatttcaatc
    10261 tatgattgca gaaattacag gtatggaaat tgcaaatgca tcaatgtatg atggagcaac
    10321 tgctgcaata gaagcatgta taatggcaat gaatcagact agaaaaagta aaatagtagt
    10381 gtcaaaaact attcatcatg aaactttgtc agtattaaga acttacttac aatataaaga
    10441 ttgtgagata gtagaaattg acttttgtaa tgaatatggg actacagata tagaaaaatt
    10501 aaaagcttct gtagacaagg atacagcatg tgttcttata caaactccta atttctttgg
    10561 aattattgaa gaaatggaag aaatagaaaa gataactcat gaaaataagg ctatgttaat
    10621 tatgagtgta gacccaatat cattaggagt tttaaaaaca ccaggtgaga taggtgcaga
    10681 tattgttgtt ggagaagctc aatcacttgg aaaccctctt aattttggag gtccttatgt
    10741 aggattttta gctagcaaat ctaaatatac tagaaaaatg ccaggaagaa tagttgggca
    10801 aagtttagat gtagaaggta aaattgcata tgtattaact ttacaaacta gagaacagca
    10861 tgtaagaaga gaaaaagcaa cttctaatat atgttcaaat caagctttaa atgctcttgt
    10921 agcttcaata tatatggcta ctatgggtaa agaaggattt aaagaggttg gtatgcagtc
    10981 tatgaaaaaa gcacattata cttacaataa gcttgtacaa actggaaaat ataagccaat
    11041 atttaaaggt aagtttttca aagaatttgc tgtacaagga aatcttaata tagaaactat
    11101 aaatgataag ctcttagaag aaaatatatt aggtggttat aatttagaat acaattatcc
    11161 agaattaaaa aattcaactt tgctttgtgt gactgagaaa agaagcaaag aggaaataga
    11221 taagttagtt ggaataatgg agggattata atgaaagaat acaatagttt gttgatagat
    11281 atatctaaaa aaggtaggaa agcatactca cttccaaagt tagatataga tgatataaaa
    11341 atagaagata tgattgacca agatatggca agacaaagtg agttgaattt gccagaagtt
    11401 ggtgaattag aattagttag acattataca ttattatcaa ataaaaactt tggagttgat
    11461 acaggttttt atcctttggg ttcttgtact atgaaatata atcctaaaat aaatgaagat
    11521 atggcagcac ttccaaactt tactggaatg catccatacc aatcttcgga tacagctcaa
    11581 ggttctcttt ctttgatgta tgatttatca agaagacttg cagaaattac aggtatggat
    11641 gaagttacac tacagccttc agcaggttct catggtgagt ttacagggct tatgattata
    11701 aaagcatatc atgaaaatag aggtgaccat aagagaacta aagtgataat tccagattct
    11761 gcacatggaa caaatccagc aagtgcagct atggcaaact ttgatgtgat tcaaatagca
    11821 tctgataaaa atggagctgt agacataaat gcattaaaag aggttttgaa tgatgaagta
    11881 gcagctctta tgcttactaa tccaagtact cttggtttat ttgaaaaaaa tataaaagaa
    11941 atagctactc ttgtacatga agcaggagga cttttgtact atgatggtgc aaacatgaat
    12001 gctattatgg ggataacaag acctggagat atggggtttg atgtggttca tcttaatctt
    12061 cataagactt tttcaactcc tcatggaggt ggaggtccag gagctggtcc tgttggagtt
    12121 aaaaaagaat tagtaccttt tttaccaata ccattaattg aaagaaaaga agataagtat
    12181 gtactaaatt atgatagaga aaaatctatt gggaaaatta aaaatttcta tggtaatttt
    12241 ggagttcttg taagagctta cacatatata ctaactatgg gaagggatgg acttaaagag
    12301 gctagtgaaa tggcagtttt aaatgctaat tatataaagg aaagtataaa agatgattat
    12361 attttgccaa tagatacctt gtgtaagcat gagtttgtat taggtggatt gccaagagga
    12421 gaggccaata taaaaactat cgatattgca aaaagactac tagattatgg ataccatcct
    12481 ccaacaatgt actttcctct tattataaat gaggcactta tgatagaacc tacagaaagt
    12541 gagagtatag agacattaga tagttttata gaagctatga aaagtgttgc taaagaggca
    12601 aaggaagaac cagaactatt gaaaactgct ccacataata ctttggtcaa aagagtagat
    12661 gatgcaaggg cagtaaagaa acctatacta acatggtcac aaagataaat aattaaaaag
    12721 tagagattta aagtataaag agaattaaat ttgtaagaaa aagctatcta acttaatcca
    12781 gtttagatag cttttttgta tattagtatg tatggtattt tctatataaa gttttaaatt
    12841 atattagtgc ttatatagaa tattgatatg atgattatgt atagatatta ataagaattg
    12901 ttgaatttat cttgccttaa ctttatttac tcttttatct taagaaaaac ttaactaaaa
    12961 tatagcgaaa ttcataaaaa tttaggaaat cttaagaatt ataatattta aatctttaga
    13021 tttatatttt attatataaa ctatagaata ataaaatata aaaattgaga attttataat
    13081 atatttgtag atttgagtat aaaaaagtgc taagttgaaa ggagtaaaat atggataagg
    13141 ttaatttaga aaggtatagt tgtgatataa atgttggttt aaataaaaat caacttcaaa
    13201 gtagaataga tgataattta attaatgcat ttgataatgg aaaaaccaaa acatataaac
    13261 aaatttttaa agacaatata tttacacttt ttaatctcat aaatattgtt ttagtctgtt
    13321 tattagtttc agttggttct tttaaaaata ccttatttat atttattgct gtaattaata
    13381 ctattatagg agtaatacaa gagataagag ctaaaagatt attggacaat ctatctgtta
    13441 tagtttcaaa taaggtatct gtcataagag aaaaagagaa aaaagagatt gatgtacaag
    13501 aattagtatt agatgatatt atgattttaa aaacaggtaa tcaaatttgt gcagattcaa
    13561 aagttttaaa tggaaaatta gaggtaaatg agtctttggt tacaggtgaa tctgatatta
    13621 ttataaaaaa taaagacgat tttttatatt ctggaagttt tgtagtttct ggagaagctt
    13681 ttgtaagagt tgaaaatata ggaaaagata actatgcaaa taagatagtt aatgatgcaa
    13741 agatttctaa aaaacataat tctcaactta gaaattcatt aaattcaata ttaaaaattg
    13801 taggtataat aataattcct ttaggaatta tattgtttgc aaaacagcat tttggaaatg
    13861 gagattctat agctacatct gtagtaggta cagtggcagc tgtaaatggt atgataccag
    13921 agggattaac attacttacg agcattgctc tagctgtagg aactataaaa cttgctagtc
    13981 ataaaacttt agtgcaagaa ctgtactgtg tagagactct tgctagagtt gatacactat
    14041 gtcttgataa gacaggtaca attacagaag gtaaaatgca agttgatgat atagtaatgt
    14101 tggaagaagt ggatattaat gaaataatgg ggaatatatg taattctctc aaagatgata
    14161 atgcaacact aaatgcaata agagataagt ataatgtttt agatacgtat aaagctaaga
    14221 atttaatacc attttcttca gaaagaaaat acagtggggt tacctttgaa aataaaggta
    14281 catatttatt aggtgcattt gagtttatat ttaaggaaaa aaataaaaaa attagaatag
    14341 aagttgaaaa atattctaaa aaaggaaaca gagtaatagt tttagctcat agtgattctt
    14401 atatggaaga taataatatt cctgaagata taaagccact agcttttata ttgatattag
    14461 ataaaattag agatgaagct aaggaaactt tagaattttt ttacaatgaa ggtgtagata
    14521 taaaaattat atctggtgac aatcctataa cagtaagtga ggtagctaga aaagcaggtc
    14581 ttaaaacggc tagtaatttt attgatgcaa caacacttaa aaatgataac gatatatacg
    14641 aagcagttga taaatacagt gtttttggaa gggtaagccc acaacagaaa aaacaaatga
    14701 ttttagcatt aaaaaaacaa aatcatacag tagctatgac tggagatggt gtaaatgatg
    14761 tgctagcgct taaagaagca gattgcagca ttgccatggc atctggaagt gatgttacta
    14821 agaatgtttc taatttagtc ttattagatt caaactttgc atcaatgcca aaggttgtta
    14881 tggaaggacg aaaggtaatt aataacattc aaagagcatc ttctttgttt ttagtaaaga
    14941 caattttttc attcttcttg tcactattaa cattattctt cttcagtaga cagtatccat
    15001 ttgttcctat acaattatca ctaattggag ctgtaacagt gggtatacca tcattcttct
    15061 tagctcttga agcaaacaaa agtagaattt caggaaactt tttactcaat atattaaaaa
    15121 atgctttacc aggagcacta tgtgtgacag taaatgtaat tattgtttac aatatagttg
    15181 aatatttagg ctatagttca agtgtatttt ctacaatgtg tgctatatta actggtgtat
    15241 gtggtctttt aatattaaga caacaatgtt tgccatttaa taaagaaaga ttatttttaa
    15301 ttgtaagtat gactattgca tttgttattg ggattctatt tttaggtgga ttattctcat
    15361 ttgtagcatt gagtagagaa ttaattctca taactatggc attagcaata ttaatgccaa
    15421 ttatgataaa agttatgtta tttgtcttgg atgagatatt agagacaatt gttccagatt
    15481 taaattaatt ttaaaatgat ataaataaga gcagttttag ttgaactgct ctttttagat
    15541 gtgtttaatt ttatataaga atatgtattt ttattaatca gctatatatg agcaacagta
    15601 gtcaacaact tcattaacct ttaaatcaaa acttgaatca ctaggaacat taaatttttg
    15661 cccttcttta taagttacaa actcattgct tccaggcagt tttacatcca tacttccagc
    15721 aagcatctcc attatttctt cttctctagt agaaaaagta tattctcctg gcaacattaa
    15781 tcctaaagtt tttctttctc cattaggcag tattattact ctgctactta ccttaccatc
    15841 aaaataaacg tttgccttct ttactgcagt aacattctta aattcactca ttagataaaa
    15901 cctccaaaat tatttgtaca tatatattat ataaaaataa tgtaaaaaat gctatatatt
    15961 tgcaaaaaaa atttagtatt tattaaaaaa attaataaga ataaaaaaga gatgaaattg
    16021 cttaaattga taaaaaatat
//
LOCUS       DAGQWR010000097        15975 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.097, whole genome shotgun sequence.
ACCESSION   DAGQWR010000097 DAGQWR010000000
VERSION     DAGQWR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15975
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.097"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            579..1229
                     /locus_tag="MIW95_002667"
     CDS             579..1229
                     /locus_tag="MIW95_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY3151980.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            1507..3573
                     /locus_tag="MIW95_002668"
     CDS             1507..3573
                     /locus_tag="MIW95_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBY3151981.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYVWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINKLKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNICILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSSMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            3590..5545
                     /locus_tag="MIW95_002669"
     CDS             3590..5545
                     /locus_tag="MIW95_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBY3151982.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKEAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDR
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLERSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            5561..6751
                     /locus_tag="MIW95_002670"
     CDS             5561..6751
                     /locus_tag="MIW95_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBY3151983.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(7599..7782)
                     /locus_tag="MIW95_002671"
                     /pseudo
     CDS             complement(7599..7782)
                     /locus_tag="MIW95_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7885..8259
                     /locus_tag="MIW95_002672"
     CDS             7885..8259
                     /locus_tag="MIW95_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY3151984.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFVSTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMGILYEED"
     gene            8261..9118
                     /locus_tag="MIW95_002673"
     CDS             8261..9118
                     /locus_tag="MIW95_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3151985.1"
                     /translation="MENILEVYNLNKTYTNFSLSNISFVLPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMSEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            9121..9759
                     /locus_tag="MIW95_002674"
     CDS             9121..9759
                     /locus_tag="MIW95_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBY3151986.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            10733..11467
                     /locus_tag="MIW95_002675"
     CDS             10733..11467
                     /locus_tag="MIW95_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY3151987.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMVLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELTPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRDIIGTLKNLIIE"
     gene            12609..13865
                     /locus_tag="MIW95_002676"
     CDS             12609..13865
                     /locus_tag="MIW95_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBY3151988.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFLLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGIAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKTLGLEKFS
                     FALSSKVVFDLPIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            13928..15121
                     /locus_tag="MIW95_002677"
     CDS             13928..15121
                     /locus_tag="MIW95_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBY3151989.1"
                     /translation="MLTSLALIFLLGMASGGIFEKIKLPSLLGMLLTGIILGSYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMNIGIFLQIKYRVVARRLSVKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGIASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     EGKSK"
     gene            15328..15708
                     /locus_tag="MIW95_002678"
     CDS             15328..15708
                     /locus_tag="MIW95_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY3151990.1"
                     /translation="MYLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKILSKLSKKRFLYVQETGKCMYYTVAIKEKTYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYI"
BASE COUNT         5916 a         1764 c         2794 g         5501 t
ORIGIN      
        1 gtatttcaaa ttgataatac aagaaaataa aaaataaaaa attttaaatt ttttatttat
       61 tgcgaaaaaa ataaaaaggt gctatacttt ttgtaacata ttattcaaat tattaagaga
      121 tagtaaatat ttatagttta atttgtagta cactagcatg atttgcagaa caatgaatta
      181 atctatagta taatagttta atttgaagta caacgattta atctatagta cactagcatg
      241 atttgaagta taacgattta atctatagta caataacttg atttgcaata caataattta
      301 atctatagta taatagcatg atttgaagta taacgattta atctatagta caataacttg
      361 atttgaagta caacgattta atctatagta caataacttg atttgcaata caatgattta
      421 atctatagta taatagcatg atttacagta caataattta aattgtagta caaaagtact
      481 atcaaataag aatttctact ttaaaaatat tactaaaagt aattaaaatc caataattca
      541 atataatatg gcaaaagaat agaaataagg tgagagtagt gaaagtaaag tcaaatttag
      601 atgaaatttt atatcataag attattgaaa gtcttattcg tggtgaatac tctgtaggtc
      661 aaaaaatatt actaaatgac ctttgtgaaa aatttgaagt aagcagaaca ccagttgtac
      721 aagcagtaaa aatgctaaat aaagatggag tgcttactat tatgacaaat ggtaaagtgt
      781 acgtaccaga gtatgaatat gatatggtaa aacaagtttg tgaaacgaga acactgattg
      841 aaacatatgc tttagaaaaa atgatgcaag aagaggaaga ggtttttcaa caaaagctag
      901 atgtaataaa aagatactct gataaatgcg aggagtacta tcgtcaagaa aaatctgtag
      961 aacttgcact agcagatttg gaattacata aagccattgt agaaggggca aataatgaga
     1021 ttttaaatga tgtatatgtt ggtatacaag gaagatttat agtagtgaac tatttaattc
     1081 gaccattaaa aaatcgtaat tatgaaggaa cagtacaaga ccattttgaa atattaaaat
     1141 tcattgaaga cagagataca aaaaaagcag tagataaact aagaaatcat attcaaggaa
     1201 ctattaaaag attctgtgaa gatgaataat aagttttgaa gttactatat acttatctat
     1261 taagttgcta aatgtagtct aagtataatc taattatagt ttaaacataa tctaaatata
     1321 atctaaatat agtgtaaata agtctaagta tgaattaaat atagattaag tatgaattaa
     1381 gcatggatta aatatacttt aaattacaaa tattacaagg aagccagaag gcttcctaaa
     1441 aaaataacct ttataaaatt ttttatttta aatttaaaat tttttatttt aaatttaaaa
     1501 taaaaaatga aagaggtggt agagagtttg aaaaaaatta ctaaagaaag gatagaaaat
     1561 actatgaaaa agctatttac gagcgaaaca tttttggaat atcaatttat aagtgattgc
     1621 caaatatcac cagatggagg atatacagca tttattgtaa aaaaagctga tatcaaagag
     1681 aatggttaca caagccaagt ttatatatta aacaataaaa ctggcgaatt aaaacaaata
     1741 acgtctataa actctgttgg agcatatgtc tgggaagatg aaaatactat cttgttccct
     1801 gctttgcgaa atgaaaaagt aaaagaagct gtcaaaaacg gtaagcaatg catgagttac
     1861 tatgctttat ctttaaatgg tggagaagca gaagaacttt ttagactccc tatcaaaggt
     1921 ggtaaattga atcctatagg aaaaggtttg tatgcagtta ttgattctta tgacaatgac
     1981 cgacctattg tagagggatt gccagaagaa gaacaacaga aaaaaataaa tgcttataac
     2041 aaaagacatt atgaacattt caaagaaatt ccttatgctg tcaatgggga gggatatatt
     2101 agcagaaaaa gaaaacgcct atatatatac gacagtaata taaataagtt gaaggcaatt
     2161 acagcaccta tgtttaatgt tgtaggtatg aaaataagtg atggaaaaat cttatatata
     2221 gggcaagagt ttaaagatgt aaaaggtttg aaaaatgggg tctatgtatt tgatacaaac
     2281 acaaacacaa atatctgtat attagaaaaa gataagtata ttataaaagg ttttgaatta
     2341 tatcaaaatc aagtgatact aaacctaact gatgctttat cttatggaaa tggagaaaat
     2401 ggagattttt atactattga tattgatacg aaagaaatga aacttctttc agggcatcaa
     2461 catcattgta ttggaaatac agtaacatct gatgttaaaa tgggtgctgg tcagacaaca
     2521 aaggtagatg gagaatatat ttattacact tctacagtag atatggactg cattattgaa
     2581 agaatacata ttccaacagg aaaacaggaa aaagtaacac agacaggttc agttgatttt
     2641 atagatgtaa aagatggcaa tattgtatgt gtgggttgtt taggaaatgg attaccagaa
     2701 gtttatacag tagagaatgg aacattgtgt aagaaaacac atttaaatga ccatatttta
     2761 gaggaatata aaatttctgt gccagaatac atagagtcaa aaggaagttc taaatgggaa
     2821 atacaaggat acgtatataa accagtagat tatgaggtgg ggaaaaaata tccagctgta
     2881 ttggcgattc atggaggacc aagattgaca tatggaccat attttatgca tgagatacag
     2941 gtatttacaa gtgcaggtta ctttgtattc ttctgtaacc caagggggtc tgaaggtcgt
     3001 ggcaatgctt ttgcagatat taggaaacag tttggagata ttgattatat agattttatg
     3061 gaatttacag atactgtact tgaaaaatat acagacattg ataaaacaaa attggctgtt
     3121 gaaggtggaa gttatggtgg atttatgacg aattggataa ttggtcatac aaaccgtttt
     3181 gctgttgctt gtgctcagcg ttctattgca aactggagta gcatggaggg tacaacagac
     3241 attggttatt atttctgcaa aggacaaaca ggtgcttctc atatggaaaa ccacgagctt
     3301 caatggaaac agtcacctct tgcttatgct gataaatgtg ttacaccgac tttattttta
     3361 catggtgaaa aagactatag atgctatatg caagaagcat tccagatgtt ttcagcattg
     3421 aaaattcatg ggtgtcctac aaaactttgt ctatttgaag gggaaaacca tgaactgtct
     3481 cgttctggta gaccaaaaca aaagctacaa agattagttg aaatgctaga ttggttttct
     3541 gtgtacataa aaaaagataa cataggacag tagaaaggag aatataaaaa tggaaaaaat
     3601 aaagattgaa agttcagata tttttgaata tatattccct catgatattt cttgttcacc
     3661 tgatggaaag catattgctt atattatatc aaatatcaac gaggaaaaag actgctatga
     3721 acatgactta tatgtgatgg acatcaaaac agaaaaacaa atacatatga ctcaaacaaa
     3781 agatgtaaca tcatttagtt ggatttctaa tacagaacta ctttttacat ctaaaagaaa
     3841 taagccaaaa gcaggtacaa cagattttta cactatttct atagaaggag gagaagcaaa
     3901 agaagcattt tctattccaa aagcttgtag cgtacctgta tcacttggaa ataaattgtg
     3961 gttattgaca acaaaaaatc caacggattc taaaaaatca gaaccagata gggcagtaga
     4021 gggtgtagat tattggacat ttacagataa accttttata agagatagag aaaatttttc
     4081 tcaaagaaga cgtgtaacat tagaattata tcaagaaggt gaaaatataa caaaagccat
     4141 tacaccaaaa ttttgtgaag tagcaggtat agacgtttct tctgataaaa acagaatttt
     4201 atatactggt cagatttatg aagattgtgc aactcctttt tctggacttt gggagtatca
     4261 tatagacagt ggtgaaacca aagaacttgt accacaagga aagtaccaaa taagtttggc
     4321 aaaatatatt ggcaaagata aagtaatgtt gcaagcttca acattagaac gttctatcac
     4381 acagaatcat gatattttta tacttgattt gagtactagt gaaataaata tgattgctag
     4441 tccagatggt atgtatgcta cattactgga tgtagatgca gtatatggtg gtggacgtag
     4501 taataaagtt ataggagata aatttattgg tgcaagaatt tgcagaacta tgacagaatt
     4561 taatgagttt gatacaaaga caggtaatat acgtatcatc acaaaagtag atgcttttac
     4621 atcttttgat atttacgaca acaccatgta cacagtaatg ttgaaagatt atgagttagc
     4681 agaaatatat agcattgata tgactacagg tacaatgaaa aaaatgactg ctttttctaa
     4741 accatattta gatactcata aggtttcatt acctgaaaaa ttgacgtttg tagcaaaaaa
     4801 taaagaagaa gttgatggtt ttgttatacc acctatagat gctaaagaag gcgaaaaata
     4861 tcctgctgta ttgttcattc atggtggacc taaatgggca tatggctata tgtttacaca
     4921 cttgaagcag tgtgtaacat caaagggaat gtatgtgatt tattgtaacc ctcatggtgg
     4981 tgacggttat ggtgaaaaat tcttagaaat ggtagaaaga tgggggtatg ttgactatga
     5041 gcatcttatg gaatttgtag atacttgtat tgaaaaatat cctgggattg atgcagaccg
     5101 cttaggggtt gcagggggaa gttacggtgg ttatatgaca aactggataa ttggccatac
     5161 agaccgtttc aaagcagcag tttctcaaag aggtattagt aacttgatta cagcttcttt
     5221 aattattgac tttggggata gaattatgaa gcagacttgt ggagataaga cgccttggaa
     5281 tcatgaggag gttctttgga atcattcacc tattaaatat gtgaaaaatg ttaagacacc
     5341 aacattgttc ttacatagtg atagggatta tagatgtttt atgggggata ctttccaaat
     5401 gtttacagca ttaaaacagc ttggtgtaga tacagaaatg tatttgttcc atggagatac
     5461 acatggattg tctagaaatg gaagaccatc taatagaatt gcacgagcaa atgctattgt
     5521 agattggttt gaaagatatt tataataagg gggttattct atgagtgata tgttattatg
     5581 gtttagtgtc gtattagttt tatttgctat tggggattta attgcaagca aaacaaaagc
     5641 aaaagtatca gctgtatttg ttacactact tttatttttg attctatttg tcacaaaagc
     5701 aataccagct gatattattg aaaaagcagg tatgaccgca gcagctagtt ggagtgttcc
     5761 aatgattatg tttagtatgg gaacaatgtt gaatgttaaa caatttatag acgaatggag
     5821 aacagtattg acagcatggc ttggaattgt agcagttata gtctgtgtct cattatgtat
     5881 tcctttgttt ggaaaatcaa cagtattgac aagcatacca gttataaatg gagctttacc
     5941 tgctactact attatgacac aagcagcact agaaaaagga ctgacacttg cagcagctac
     6001 agcaacagtt gtatttgcaa ttcagaagtt tattggtaca ccaatagctt caagagcagc
     6061 gttacaagag gcaaaccgtc ttttagtaga atatcatgaa gcaaaatcaa aaggcattga
     6121 tttagcaaat gttgatactg ataagaaaga agcagagaat acaggcacaa aagtaaaaca
     6181 agctttctgt gaaaaatacg ataaatatta ttctacaaat gtttgtatat tttttatagc
     6241 actattctct tatctagggt atgaactttc agagattatt catgtaaatt attctattgt
     6301 atgtcttgtt gttggtgtta ttgtaacacg tattggtatt gtaccaaaag acattttgga
     6361 aaaaggaaaa atcaagggat ttattaacat ggttgtgttt gcagctgtta ttccttcttt
     6421 agcaaaagtt tctttgacag atttaattag tctttttgta ccaattgtgg gtatgtttgc
     6481 agcttctatc attggtatat tccttatgat gaaggtgtta ccaggttgga aaattatcgg
     6541 ttctaaacct cttgcttttg gagtagggtt ttgtcagatg ttgggattcc caactacata
     6601 tttaatttca aatgaagtgt gtaatgctgt tggagaaacg gaagaagaaa gagcatattt
     6661 gatgtctaag attatgccta agctggtagt tggtggtatg gcttgtatga tttctattgt
     6721 agtagctggt cttatggtac caatgttgta gataggtttc atagttaaaa agtggtgtta
     6781 atttatagga gaagctaaaa agtagtatta atttataaaa taattttata ttagttaatt
     6841 tgttataaga atattttctt tttatgatta taattttgat tttataagag agcagatttt
     6901 ttatttgcta tgctaatagt tataatttgt ttttatttgc taaaatgcaa ctttaatttg
     6961 atttatgtat aatatagggt atttaataat atttttgaag aatttttata aaaaattaaa
     7021 aagaggagaa aaaagggagt gccacaaagt gaacttttta gttctaaggc actctttttt
     7081 gtacgaaatc gaatatattt tcaacgtttt aaaagtgaga aagagactta tctctagttt
     7141 gggatagcca aatttaatta ttaaaattat tttttgtttt atttatcaaa gagtattcgt
     7201 gttttagaag ttagtatttt ttattttata tatccaaggt tttccgtttc taatgttttc
     7261 ttttttaaaa taattagggt attcaattat agtaaaaggt ggatggtatt ttggaatgat
     7321 attatttaaa gcacagtatt gaatgttgaa atcctcacat tttagagtat tatcttttgt
     7381 aatcaaaaaa atcttctata cttatagcaa ggttacttct gcaaatattc catgcatcat
     7441 aatatccaaa tttaaatttt tttaatatag agtttacctc tagtctacac tcttgaataa
     7501 ctctttcctc tatccaaatt cttataaact catcccattt attactatct atttattact
     7561 atctaaattt tttagtatgt ttgtaattgg aaatggattt attcctttat gttttttata
     7621 aatagtttat aacttaattt attattaaac aaatcattca tacagaatta ctttttcttt
     7681 tagatattca attcttactt ttaataaata tttaatacat tctatcttat gtggttttaa
     7741 aaagtgttta tggctatcaa taatctctaa tatttcattc atatttattt tgtcatagta
     7801 atattttaca tcttttatat tgacaaaaaa gtgctctaag tgtatagtgt gtatataata
     7861 acatcacact ggaggtgata aaaaatgaaa gtgctcattt ctaacacatc agataagcca
     7921 ttataccaac aaattaaaga gcaaattaaa gaagcaatat ttactgaaga actgaaagaa
     7981 ggggatgcaa tgccctcaat aagaaatttt gctaatgatt taaaagttag tattttgact
     8041 attagacgag tgtatgaaga acttgaaaat gaaggttttc ttacacgcca agttggaata
     8101 ggaacatttg tttcaactgg aaatttagaa atactaagag actcaaagag gcgtatagtt
     8161 gaacaaaaaa tggcagatat gattaaggat gcaaaagcac taggaattac acagatagag
     8221 ttacaagaaa tgatgggtat tctatatgag gaggattaat atggaaaata ttttagaagt
     8281 ttataactta aataagactt ataccaattt ttcattatct aatataagct ttgtattacc
     8341 agaagattgt ataactggat ttataggtgt taatggagca ggaaaaacaa caacaattcg
     8401 ttcgatattg gatgtcgtaa agcgtgatag tggaaaaata aaaatctttg gtaaagattt
     8461 cgatggaaat gagaaggaat taaaaaatcg aattggagtt gtatttgatg attcatattt
     8521 ctatgaggag ttgtcaatgt cagaaatgaa aagtattatt gcaccttcat atgataattg
     8581 gtgtgaagaa gattatcaac aaaatatgaa gcgttttaat cttaatccaa aacaaaagat
     8641 tgatacactt tcaaaaggaa tgaaaatgaa atttgcttta gcattagcat tatcgcataa
     8701 agctgagttt ttaattatgg atgagcctac aagtggttta gacccactaa tccgtagtca
     8761 gatacttgac atactaacag aatatatgaa gaaatgtggt cgtggtgttt tcttttctac
     8821 tcatataaca tctgatttag acaaaatagc agatatgatt gttatgattg acaatggacg
     8881 tattatatta gagcgtgaaa aagatactct attggatgaa catagaaaag taaaagggga
     8941 tattcaattt ctaaatgatg aaatcaaaaa attattttta aatttagaaa ttacaggtta
     9001 tggttttata gggattacct caaaaattaa tgaagtgcaa aaaaatatgc cagatatttt
     9061 aatagagcgt cctacaattg aagatattat gcttgcatat attgaaagga gagagtaatt
     9121 atgttgattc atttagttaa aaaagatatt ctgattgtaa aaaaatatgt attgttgata
     9181 ttgtttattg caataggaat cccattattt atattatggc gtttacctcc atttgcacaa
     9241 atattgggat ttgtactatc aattatattt tcagaattta tgctgtgcca gtatctatta
     9301 ttaaaggaga gtcaatattc aaaagcatct gtattacttt gttcaactcc ttatcctaga
     9361 cgtggcttag ttcaatctaa gtatgtttta tttgcaatgg tatttatcta ttgtacatta
     9421 gtttactgga ttgaaaattt acttattcca caaataggaa cttttgactt aacatatatt
     9481 ttgattgttt tattaataag ttctattatt tatggagttt atatgcctat tcaatacaaa
     9541 ttaggatatg aaacaacaaa attctttttc gtgatgataa tcatggcttc atcatttgta
     9601 ttaccattaa ctatagctca cagcaatttt gaaattccat ttatatatca gtatccatcc
     9661 atgatagtga atattgtatt gttcctttta agtttaatta tactgttagc ttcaatgttt
     9721 atttctatta aaatttttag taataaggag cttgtataag gtaaggggga attttatatg
     9781 ccacatttgc cagattgggc tgttttactt tgtatcttac ttccaatagt atataaagct
     9841 tgtcagatat ttaaaagtaa aaaatagagt acatatttat attgccattt ttattgagtg
     9901 aagttagatg gtatgaattt aaaaggttac ttgaaaataa agttacttgg tgtgggagag
     9961 taacatcata atattataga ttatatgttt cttgtgaatc aatttgtatt ttttaataga
    10021 taatatccag taattttatt ttccattgat tattttcaat tatgagtatg attgtgtgta
    10081 ttgtatacca tcttttaagg tgtttttaaa aaattatatc gtcaaaatat aaatctaagg
    10141 aatcctataa ataaagcttt tcaattttag aaaactaata aaaagtagga agttaataaa
    10201 aagaagctgt ctcaaaatag agataagctt ctttttcatt gctgaaatga cgaaaatata
    10261 attgatttaa tataaaaaag agtgctttat gaactattta ttttgaacag tagatacact
    10321 tgcactcaat ttagttactc ctaagcttta tgaactaaaa gtttattttg aagcctattt
    10381 ttctaaataa tcatctatat acagtattgc gtttctttta tttattcata atttattttc
    10441 ttattaaagt ttttctatat tactttactt ttaattttat acgaattatc aaaaataaaa
    10501 tagttattag aaaaaaataa aagtaattgc aaagattgca attacttact tctatattaa
    10561 ttactatacc ataaaataac aaaaaaatca agtcgagtaa tccttcaact taagtgatat
    10621 actaggtatt aataattttt aattaataaa atataactag aggaaaatct acattttctc
    10681 aatttcttta attaaattga cctctaatta agtaattata aggagaaaaa ttatgaattt
    10741 agcaaaggga ttagatgttt taaaaatttc atcaaatgta ttcggtgaag ataaagttat
    10801 gtatattcct gtaatctaca cagaagatga tgcaacactt attgacacag gtcttccggg
    10861 tcaaggtgat ttaataattg atgctcttaa taaaagtaac acgagctttg ataggttaaa
    10921 aaatataata attactcatc atgatataga ccatatagga aacataaatt atctaagaga
    10981 aaaatctaag aataatatca aagtatatgc atacaaaagt gaagtcagtt atataacagg
    11041 agaagaaact ccttttaaac tttatatgtt ggaacaaatg gtagataaaa ttgatgataa
    11101 gatgcttagt atgctaaatg ttatggtact tggatttaaa tcatcatata ctaaagtaga
    11161 tgtgtcatta gataaccatg aaaaattgaa tctaggtgag gaaatagaag ttatacatac
    11221 aggaggtcac actagaggtc acatatgtct ttatttaaaa gagtctaaag tacttattgc
    11281 tggagatttg ttacaggtag aaaatggaga acttacgcct gttgatgtaa tgcacagcaa
    11341 caaacaagag ttaaaagatg ctattaaaaa tataagtaat tatgatatag aaacgatagt
    11401 ttttagtcat ggtggattat atcaaagaga cataattgga acattgaaaa acctaattat
    11461 tgagtagtac tatatgattt acaataattt cttaaattac ttaaaattat atctaaagat
    11521 gtagaatcaa aattaaatat aagtaataag aattttgctt taaaattata cttaagtgtt
    11581 aagtatgata cgtagtatta caagtatttt agaatataat tttttatagt aattttaagg
    11641 ttctacaata cttcagttga aaaatattta acagcatagt ataataggaa agaaaatatt
    11701 ttaaataaac tttagtatac aaagtgaaga aaaatattta aattatatat aatattgtag
    11761 agtgaggtaa aatatttaag ttatatatca taatacagag tgaagaaaaa tatttagata
    11821 atgtaccata atacaaggtg agtaaaaata tttaaagcat gtataataat gcaaagcgaa
    11881 gaaaaatatt taaataatgt accataatgc agagtgagta aaaatattta aataatgtgt
    11941 tataatacaa agcgaataag attatttaaa gcatgtatta taatataaag tgagtaaaaa
    12001 tatttaaatc atgtatcata atataagcag agtgaagaaa aatatttaaa taatatatca
    12061 taatgtagag tgaggtaaaa tatttaagtt atgtatcata atacaaagta aagaaaatca
    12121 tttaaattat atatcataaa aataatatga aatattgaac actcataata ttggaggctt
    12181 taatatttta attgataggt tacattattt ataacattaa aaatacaatt tacataaaat
    12241 agacatatga aaaataaaaa attatataat aattatggga atgaggttct cccttggatg
    12301 ataatatcct aaactgccaa tgagctgatg acttctatga tatcatagat gccaccagct
    12361 ttttttgttg tttaaatcga taaaatggag gtgaattact tactaaattt ttatttttta
    12421 aatttgaaga aatatttaat taatagaaaa aacagtagat tatatgttaa ttattttatt
    12481 aagaatgggt taagagtata cctatatcct atcaatttaa aaaacaatta tgatagtcat
    12541 tacattgact acagttgatg caataaaact ttcaaaaaat cttatgatgc aaatagaaag
    12601 gaattaaaat gatagaaaaa tttaaaactt taatatggat taaaatgata ataagaatta
    12661 aaagcgttta taaaacatat ggtggactat ttttgttagg actcataggt ataccagttg
    12721 gtgcaattat aggtcttata gacaccatat ttggaacggt tcttctaaaa gttacagata
    12781 ttcgagaaac atatccaatg tatctgatac catttttagc agtagtagga gttgttattg
    12841 cttattgcta ttttaaattt ggaggaaaaa gtagtaaagg aatgaatcta atatttgagg
    12901 tagggcatgg agaagaagaa attattcctt taagattggt tccatttata atatcaggta
    12961 catggttaac ccatttattt ggtggtagtg caggacgaga aggagttgct gttcagattg
    13021 gtgcaacttt ttcacattgg gtaggtaaaa ggttgcctat aaaaaatgct tctagtattt
    13081 ttctagtaac tggtatagca gctggttttg caggattatt tgagacacca atagcagcaa
    13141 tactatttgc tatggaggtt ttggtagctg gttcactgga gtatcaatcg ctttttcctg
    13201 cgtttactgc ttcttttaca gcaagtgcag tatcaaaaac tttagggtta gagaaatttt
    13261 cttttgcact ttcttctaaa gttgtatttg acttaccaat attttggaag cttattgttt
    13321 taggaattat ttttggaatg gttggtggag catttgcatg gtgcctgaaa ttgtcaaaaa
    13381 gaaaaatagg taatagatta aaaaatccta tgataaggat tgctattatt ggagtatgtc
    13441 ttagtgtatt attcctttta ttttataaag gaagatattc aggtttaggg acaaatttaa
    13501 ttcaaaatag tttttatggt ggagaaattt attcatttga ctggctgtta aagtttatac
    13561 ttacaatact gactttgtca gcaggttttc aaggaggaga ggttacacct ttgttttcca
    13621 ttggagctag tcttggtgta ttgttagcag gattttttaa tttgccaatt gaattagttg
    13681 cagcgcttgg ctatgctagc gtctttggca gtgctacaaa tacttttttt gctcctgtat
    13741 ttataggggc agaagttttt ggatacagct atttacctta tttctttgta gtatgcgcta
    13801 tttcatatat atttaatatg gataaatcta tttattcact acaaaaaatt tcaacgaaac
    13861 aataaataaa acaacataat gtatgattat aagagaaaga atattagaga aaggataggg
    13921 taggaatatg ttgacaagtt tagctttgat ttttttatta ggaatggcct cgggaggtat
    13981 ttttgagaag ataaaattac ctagcttatt gggaatgtta cttacaggaa tcatattggg
    14041 ttcatatgtt ttaaatttga ttgataattc aatactagat atttcttctg accttagaaa
    14101 gattgcattg attattatat taacaagggc tggattatct ttagatataa acgatttgaa
    14161 gaaagtggga agacctgctg tacttatgtg ctttattcca gctacttttg aaattatcgg
    14221 aatgatagtt ttagcaccaa aattactagg cgtttctata ttggaagcag cagtcatggg
    14281 tgcagtagta ggagcagttt ctcccgcaat tattgtacct aaaatgctta aattaatgga
    14341 agaaggttat ggtacagaaa aaagtattcc acagatgctt ttagcaggaa cttctataga
    14401 cgatattttc gtaattgtga tgtttacagt attcacagga ttggcacaag gaaatagtat
    14461 atcagctatt agttttttac aaattccagt atcaattata ttaggtgtaa tagttggagc
    14521 agtgattgga atctgtttag cagtattttt taagaaggtt catatgagag attctgctaa
    14581 aggggtgttg cttttaagta tctcattttt aatgataagt ttggagactg ctttagaggg
    14641 tattgtgcca ttttctggat tattagcagt tatgaatatt gggattttcc ttcagataaa
    14701 atatcgtgta gttgcaagaa gattgtctgt aaagtattca aaactatggg ttggtgctga
    14761 aattctcttg tttgttttgg taggggcaac agtagatata agttatgcat ttaaagcagg
    14821 aattggagct gtaattttaa tatttggagt tcttttattc cgtatggtag gagtattctt
    14881 ctgccttatt aagacaaatt tgacaataaa agaaagattg ttctgtatga ttggctatat
    14941 tccaaaggct acagtacagg cagcaatagg aggtgtacca cttgcaatgg ggatagcatc
    15001 tggacaactt attttgacat tagcggttct cgctatactt ataactgcac ctcttggagc
    15061 ttttggaatt gatgtaactt ataaaaaatt attaacttct gtagaaggta agagtaagta
    15121 agaataaaaa taaagattat tttaagtgat aaattatatt ttggaaaaaa ctatattatt
    15181 gagtacaatg ttagaaaatc atttgagtga ttacagaagg tagaagttta tatcggattt
    15241 taaggcatgt gatgattatt agaattgatt ttgtgaaatt gttattgtaa tatttaatta
    15301 tatagataaa gaagcggagt gataactatg tatttaaaaa aattatcaaa attagagtta
    15361 gttattatga aatttatttg gaatttagat ataaaaacca attcatatga gattattgat
    15421 tatatgaaag aagagcataa tttaccagaa aaagtggctt taaaaatttt atcaaaatta
    15481 tcaaagaaaa gattcttata tgttcaagaa acaggcaagt gtatgtatta tacagttgca
    15541 ataaaagaaa agacatattt agagtttata tcaagaaatg tgcaaaatct tttgaagaat
    15601 aattttataa ggaatttatt agtatcattt cacgaagagg agctaacaga ggaaaagata
    15661 aagtcgttag aaaactgggt agtaaattgg gaagaagctt atatataaat gttattattt
    15721 ttattacaat ttagataatt ttggttttgt aagctctgca actattttta gatggttgca
    15781 gagcttattt ttatttttta ttcccttaag caatacttaa aagtaaacgc atttatattt
    15841 actaacttca atcacttcta atacttagat tactttttaa aaatattttg atatgtcatc
    15901 tctatagtaa tctagaactt gtttctattc tttatataaa gtttttatga atgatatata
    15961 taatattcgt tcatt
//
LOCUS       DAGQWR010000098        15959 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.098, whole genome shotgun sequence.
ACCESSION   DAGQWR010000098 DAGQWR010000000
VERSION     DAGQWR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.098"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            235..384
                     /gene="rpmG"
                     /locus_tag="MIW95_002679"
     CDS             235..384
                     /gene="rpmG"
                     /locus_tag="MIW95_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBY3151991.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            410..631
                     /gene="secE"
                     /locus_tag="MIW95_002680"
     CDS             410..631
                     /gene="secE"
                     /locus_tag="MIW95_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBY3151992.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            651..1193
                     /gene="nusG"
                     /locus_tag="MIW95_002681"
     CDS             651..1193
                     /gene="nusG"
                     /locus_tag="MIW95_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBY3151993.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDVDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1229..1654
                     /gene="rplK"
                     /locus_tag="MIW95_002682"
     CDS             1229..1654
                     /gene="rplK"
                     /locus_tag="MIW95_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBY3151994.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1724..2422
                     /gene="rplA"
                     /locus_tag="MIW95_002683"
     CDS             1724..2422
                     /gene="rplA"
                     /locus_tag="MIW95_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBY3151995.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2474..2602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2645..3151
                     /locus_tag="MIW95_002684"
     CDS             2645..3151
                     /locus_tag="MIW95_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBY3151996.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3209..3574
                     /gene="rplL"
                     /locus_tag="MIW95_002685"
     CDS             3209..3574
                     /gene="rplL"
                     /locus_tag="MIW95_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBY3151997.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3966..4754
                     /locus_tag="MIW95_002686"
     CDS             3966..4754
                     /locus_tag="MIW95_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBY3151998.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5225..8941
                     /locus_tag="MIW95_002687"
     CDS             5225..8941
                     /locus_tag="MIW95_002687"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBY3151999.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGQSFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     VEATEDEDIENLEEFTEDDLFEEEIDFESDDFDM"
     gene            8983..12468
                     /gene="rpoC"
                     /locus_tag="MIW95_002688"
     CDS             8983..12468
                     /gene="rpoC"
                     /locus_tag="MIW95_002688"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBY3152000.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVLVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12757..13179
                     /gene="rpsL"
                     /locus_tag="MIW95_002689"
     CDS             12757..13179
                     /gene="rpsL"
                     /locus_tag="MIW95_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBY3152001.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13302..13772
                     /gene="rpsG"
                     /locus_tag="MIW95_002690"
     CDS             13302..13772
                     /gene="rpsG"
                     /locus_tag="MIW95_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBY3152002.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13821..15887
                     /gene="fusA"
                     /locus_tag="MIW95_002691"
     CDS             13821..15887
                     /gene="fusA"
                     /locus_tag="MIW95_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBY3152003.1"
                     /translation="MARKFPLEKTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFINKMDILGADFYNVISMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6246 a         1985 c         3249 g         4479 t
ORIGIN      
        1 atggattaaa aggaataaca aaaagagggg ttaggctcct ctttttttaa catagaaata
       61 caaaaacatt gacacagcat aggattttgt gataacttat acaaggtaat gattatagga
      121 agttaataat atcttgaata ctgtagattt ttaataaata catattttaa agatattata
      181 ttcacatata ttaacaatat atattattgt attacaaagt tggaggtgta tcagatgaga
      241 gttaaagtaa ctttagcatg tacagagtgt aagcaaagaa actacaacac tactaagaat
      301 aagaaaaata atccagacag aatagaatta caaaaatatt gtagattctg taaaaagcat
      361 acaactcata aagaaacaaa atagtttttt ataaggatgt gagtatggaa tggctgccca
      421 aaaaaatgac ggtgcaaaaa ctaaaaaaag gtttagttta tttggatatt taaaagaaac
      481 taagcaagaa ttaaaaagag ttacatggcc gacaaaaaag gaactgttta aaaacacaag
      541 tatagtttta actgtagtta tttcatgcac tatattagta tggggtatag acactatatt
      601 atctggtgca ctagcgttat tactaaaata gacgaaaaga ggtaagttaa atgtcagaat
      661 tacaagaagc aagttggtat gtagttcata cctattcggg acatgaaaat aaagttaaag
      721 ctacaattga gaaagcagtt aaaacaagag gtatggaaga ctgcataaga caagtagttg
      781 ttccaacaga agaggtagtg gaaactacta aaactggaaa agaaaaaact agacaacgta
      841 aagtttatcc aagttatgtc ttggttaaaa tgataataac tgatgaatct tggtatgtag
      901 taagaaatac taagggagtt acaggtttcg ttggacctgg ttcaaaacca gtaccattaa
      961 gtgaagatga agtcaaagct atggggatag atacaacaga ccctaaagta gtaaatagcg
     1021 atgttgattt tgaaatagga gacacagtaa aagtatctca aggaccattt agtggacaaa
     1081 taggtaacat agaagaaatt gacttagaaa atagagaagt caaagtatgc ataaatgcat
     1141 ttggtaaaag gactctattt gtaatagagc ttgaaggtat agaaaaaatc taatactata
     1201 tagaaaaatc tgaggaggtg cgctcaaaat ggctaaaaaa gttataggtc aaataaaatt
     1261 acaaatacct gcaggaaagg ctactccagc gccaccagtt ggaccagcat taggacaaca
     1321 tggtgttaat ataatgggat tcacaaaaga atttaatgct aaaactgcag atcaagctgg
     1381 aatgataata ccagttgtta taactgtata tcaagataga tcattcagtt ttataacaaa
     1441 aactccacca gctgcagttt taattaaaaa agcattaaac ttaaaatcag gttctggaga
     1501 accaaacaaa aagaaagttg ctaaaatgac ttcagctcaa gtaagagaaa tagctgagtt
     1561 aaaaatgcct gacttaaatg ctgcatcagt agaagctgct atgagtatga tagctggtac
     1621 tgctagaagc atgggtgttg taatcgaaga ctaattaaaa gatacacttt ttttataaag
     1681 tgggaggcga gaagccgaaa ttaaccacaa ggaggaaaat aaaatggcta aaaaaggtaa
     1741 aagatatgca ggtgcattac aaaaagtaga tagaactaaa ttttatgatg catcagaagc
     1801 attaacatta gtttcagata tagctggagc taaatttgat gagacagtag aagcacacat
     1861 taaattaggt gttgactcaa gacatgctga ccaacaagta agaggtgcag ttgtattacc
     1921 tcatggaact ggtaaaacaa aaagagtttt agtttttgct aaaggtgaaa aagctaaaga
     1981 agctgaacaa gctggtgctg actttgtagg agctgaagaa ttagttcaaa aaatacaagg
     2041 tgaaaactgg tttgactttg atatagtagt tgctactcca gatatgatgg gtgtagtagg
     2101 tagattaggt agagtattag gacctaaagg tttaatgcca aaccctaaat caggaacagt
     2161 tacatttgat gtagctaaag ctatagatga aataaaagct ggtaaagttg aatacagatt
     2221 agacaaaact aacataatac acgttccagt aggaaaagta tcatttggtg gagaaaaatt
     2281 aactgaaaac tttactgcat taatggatgc tataataaaa gctaaaccag ctgctgctaa
     2341 aggacaatat ttaagaagta taacagtagc ttcttcaatg ggacctggag ttaaaataaa
     2401 cccagcaaga acagctgaat aataaaactg ttgacacagg ttgacaaaaa tgttattata
     2461 tcacttgtca atgtaaaaaa gaatagattt gccgtagata gtgggtgcga aagcttaata
     2521 tcccgccgag gttttagata aattgagatt actaaagctt ttgatgtcta cttttggcaa
     2581 caaaagcttt ttataatgtt gcggtaaata ttaagcctat ttggtgaagg aggtgcacat
     2641 ctatatgaga aaagctatag aaattaaatc agaagttgtt tctgagatag ttgaaaagtt
     2701 acaaaaatct tctgctgctg ttgttgtaga ttacaaagga ttaacagttg aagaagtaac
     2761 tgagttaaga aaacaaatga gagaagctgg tgtagattac aaagtataca aaaatacttt
     2821 agttagaaga gcagctaaag aagttggtat agagcaattt aatgatgaat tattagttgg
     2881 aactaatgcc atagcatttg gatatgatga tccagttgct ccagctagaa tattaaaagg
     2941 attcatggat tctcatccaa aaatgaaatt aaagatgggt attgtggaag gtgcattcta
     3001 tgatgaatct aagattgttg aaatggctaa cataccatca agagaagttc ttatcgctaa
     3061 attacttggt agcttaaaag ctccagtatc aaacttcgca tacttaatag atgcaatagc
     3121 gaaaaaggca gaaggtcaag aagaagctta attgcttaac taaaaaatta taaatattaa
     3181 tttgaaataa aattcggagg tgctaaaaat gacaatagaa caaatattag aagctataga
     3241 aaatatgaaa gttttagaat taaatgaatt agttaaagct gctgaagaaa aattcggtgt
     3301 atcagcttca gctccagtaa tggtagctgg tgcagctgct ggtggaccag ctgctgaaga
     3361 aaaaactgag tttgatgtag tattaactga cgttggttct tcaaaagttg gagttataaa
     3421 agcagttaga gaaataacag gattaggatt aaaagaagct aaagaagtag ttgacaatgc
     3481 tcctaagaca gttaaagaag gagcttctaa agaagaagct gatcaaataa aagagaaatt
     3541 agaagctgct ggagctaaag tagaagttaa gtagtttctt taaaaagata cccattgggt
     3601 atcttttttt atttataaaa aactataaag aaagtatttg gaattataaa gaaagtgttt
     3661 ttataaaata aaaaaaattt atataatagt actaaattaa gggtatcata tatggtgtaa
     3721 tggtatttta atttaacatt attatcaaat taaaatataa acaattgttg taatgtctgt
     3781 atataaagta tataattaaa ctaaaatgta aaattttaca ttttagttta aaaaaataca
     3841 cgagtgtttt ataaataaaa tgtaataaat aattaattaa caaatataaa ttttaaaata
     3901 agttagatat ttagaaaaat cgaaatttgt aggatttaaa ggacatattc aaggaggagt
     3961 taataatgga aaaattacaa ggaaaaattg cagtagttac tgcagcaaca aaaggtattg
     4021 gattagcatc agcagagata ttagcaaaaa atggagcaac tgtgtactta gcagctcgtt
     4081 cagaagaatt agctaatgaa gttataaata aaataagtgc agaaggtggt tgtgctaagt
     4141 ttgtttactt taatgctcgt gaagaagaga cctttacctc aatgatagaa gaggtagtta
     4201 aaaaagaagg taagatagat atacttgtaa acaattttgg ttcaacaaac ccttctcttg
     4261 acaaagacct tgtaactggg gatacagata atttctttga cacagtaaat actaatttaa
     4321 aaagtgtgta tttaccatgt aaagcagcaa ttcctcatat gataaagaat ggaaaaggaa
     4381 gtatagtaaa tatatcaagt ataggttcag tattacctga tttatctaga atagcttact
     4441 gtgtatcaaa agcagcaatc aactcattaa ctcaaaacat agctacacaa tatgctaaag
     4501 acaatgttag atgtaatgct gtacttccag ggcttattgc aactaaagca gcattagaca
     4561 atatgtctcc agagttcata aaagaatttt taaagcatgt tcctttaaat cgtatagggg
     4621 aaccagatga tatagcaaaa gctgttctat tctatgctag tgatgactca tcatttataa
     4681 caggggattt acttgaagtt gcaggaggat ttggtttacc tactccacaa tttgcagata
     4741 atatattagg ataattaaat tactaatatg caatgaaagc ttatgcatta gttatagtag
     4801 aaatgatatt tttataaagg ccatagatat aatttatatt ggaaaggttt gaggttcaaa
     4861 attccaagtt aaatttattt atggtcttta ttttgtatat tttaataaat tgtatatatt
     4921 tgtataaatg taattattta caatcatttt aatatggaat tataattaaa taggggggat
     4981 ggtacttgta taaaattttt tatagaaaat tagacctata aatgaaaaat atggatttta
     5041 tattggaaaa ttgtacataa tacataatat atgcttaaaa taatttacaa aaaatcaaaa
     5101 ataatgcata gtttacatat aagataaaaa atatattgaa aaatacttga cattatgcta
     5161 aaattattag atgcgttaga atgaactatt gttttatttt taattattga gaggtgaaaa
     5221 gtgaatgcca catcctgtca cgataggtaa aagaactaga atgagctttt ctaaaattaa
     5281 ggaaatagct gatgtgccaa atcttataga aattcaagta gattcctatg agtggttttt
     5341 aaaagaaggt ttaaaagaag tgtttgatga tatttcacca atagaagatt atactggtaa
     5401 tcttatattg gaatttgtcg actattctct agatgataaa ccaaagtacg atatagaaga
     5461 gtgtaaagag agagatgcta catattgtgc acctctaaag gttaaggtaa gacttataaa
     5521 taaagaaaca ggtgagataa aagaacaaga agtgtttatg ggtgacttcc ctttaatgac
     5581 agaaagagga actttcgtta taaatggagc agaaagagtt atagtaagtc aattagttag
     5641 atctcctggt gtttactatg cggaagaaag agataaaaca ggtaagagat tgatttcatc
     5701 tactgtaatt cctaatagag gagcatggtt agaatatgaa acagattcta atgatgtaat
     5761 atctgtaaga gttgatagaa caagaaaaca accagttaca gttttactta gagctttagg
     5821 aataggaaca gatgcagaga taattgacct tttaggagaa gatgagagat tatctgctac
     5881 attagaaaaa gataatacta agacagtaga agaaggtctt gtagagatat acaaaaaatt
     5941 aagaccaggt gaacctccta ctgtagaaag tgcatcatct ttattaaatg ctttattctt
     6001 tgacccaaaa agatatgacc tagcaaaagt tggtagatat aaattcaata agaaacttgc
     6061 tttatgttat agaataatga ataaaatttc agcagaagac ataataaatc cagaaactgg
     6121 agaagtattt gtaaaagctg gagaaaaaat aagctatgat ttggctaaag ccatacaaaa
     6181 tgcaggtata aatgtagtaa atctactaat ggatgatgat aaaaaagtta gagttatagg
     6241 aaataatttt gtggatatta agtctcacat tgattttgac atagatgatt taaacataaa
     6301 agaaaaagta cattatccaa ctttaaaaga aattttagat ggatatagtg atgaagaaga
     6361 aataaaagag gctataaaat caagaattaa agaacttata ccaaaacata tattattaga
     6421 tgatataata gcttcaataa gttatgagtt caatatattc tataatatag gaaatattga
     6481 tgatatagat cacctaggaa acagaagaat aagatctgta ggtgaattgt tacaaaatca
     6541 agttagaatt ggtctttcaa gaatggaaag agttataaaa gaaagaatga cggttcaaga
     6601 tatggaagct ataacgcctc aagcgttagt taacataaga ccagtttcag ctgcaataaa
     6661 agaatttttt ggtagttctc agttatctca gttcatggat caaacaaatc ctctatctga
     6721 gttaacgcat aagagaagat tatcagccct tgggcctgga ggtctttcaa gagaaagagc
     6781 tggattcgaa gtgcgtgacg ttcaccattc acactatggt agaatgtgtc caatagagac
     6841 tccagaggga ccaaatatcg gtcttataaa ctctctagga acttatgcaa aaataaatga
     6901 atttggattt atagaatcac catatagaaa atttgataaa gaaacatcaa cagttactga
     6961 tgaaatacat tatttaactg ctgacgaaga agatttattc gtaagagctc aggcaaatga
     7021 gccattaaca gaagatggta aattcgtaaa ccatagagtt gtttgtagaa ctgtaaatgg
     7081 tgctgttgaa atggttcctg aaagtagagt agattacatg gatatatctc ctaagcaggt
     7141 tgtatctgtt gccactgcta tgataccttt cttagaaaat gatgatgcca accgtgccct
     7201 tatgggagcg aacatgcagc gtcaggcagt gcctctagtt agaagagaag caccaattat
     7261 aggaactggt atagaataca gagcagcaaa agactctgga gcagttgttg ttgctagaaa
     7321 ttctggtata gcagaaagag taacagcaga tgaaataatt ataaaaagag aagatggaaa
     7381 cagagataga tataatctac ttaagtttaa acgttcaaac tcaggtactt gtataaatca
     7441 aacacctata ataaataagg gtgaccagat aataaaaggt gatgttatag cagatggtcc
     7501 agcaactgat ttaggagaag ttgcacttgg aagaaactgt cttatagcat tcatgacttg
     7561 ggaaggttat aactacgagg atgctatatt aataaatgaa agattagtta aagaagatag
     7621 attatcaaca attcatatag aagaatatga atgtgaagct agagatacaa aactagggcc
     7681 agaggaaata actagagata tacctaatgt tggagatagt gcaatcaaaa acttagatga
     7741 tagaggtata ataagaatag gtgcagaagt agactcaggt gatatattag ttggtaaggt
     7801 aactccaaaa ggagaaactg aacttactgc tgaggaaaga ttacttcgtg cgatattcgg
     7861 agaaaaagcc agggaagtta gagatacttc acttaaagta cctcatggtg aatctggtat
     7921 aatagttgat gtaaaagtat ttactagaga aaatggagat gatttatctc caggagtaaa
     7981 tgaattagta agatgttata tagctaaaaa gagaaagata aaagttggag ataagatggc
     8041 tggtcgtcat ggtaataaag gggttatctc gagagtatta cctgaagaag atatgccatt
     8101 catggaaaat ggaacgccat tagatataat acttaaccca caaggtatac catcacgtat
     8161 gaacatcggt caggttctag aagttcattt aggacttgct gctaagacat taggatggta
     8221 tgtagcaaca tcagtattcg atggtgctaa tgagtatgac ataatggatg ctcttgaaga
     8281 agctggatat cctagagatg gtaaattaac tttatatgat ggtagaacag gtcaatcttt
     8341 tgacaataga ataactgttg gatatatgta ttacttgaaa ctacatcact tagtagatga
     8401 aaaactacat gcaagaagta ctggaccata ctcattagtt actcaacagc cactaggtgg
     8461 taaagcacaa tttggtggac aaagatttgg agagatggag gtttgggctc tagaagcata
     8521 tggagcggct cacatacttc aagagatact tactgttaag tctgatgatg ttgtaggtcg
     8581 tgttagaact tatgaagcta tagttaaagg tgaaaacata cctgagccag gaatcccaga
     8641 atcatttaag gttcttataa aggaacttca aagtttatgc ttagatgtaa aagtattgac
     8701 agatgaagac caagaaatag aagtaagaga atctgtagat gaagatgata cgataggtga
     8761 atttgagcta gacgttgtaa atcacatggg agaagttgaa gaaagtaata taatagaaga
     8821 aattgaagat gattttgtgg aagccacaga agatgaggat atagagaatt tagaagaatt
     8881 cactgaagat gatttatttg aagaagaaat agattttgaa agtgatgact ttgatatgta
     8941 aatttaatta atgtaaatct caaatagaag ggagagaact ccttgtttga attaaacaat
     9001 ttcgagtcga taaaaatagc attggcttct ccagaaaaaa taagacaatg gtctagagga
     9061 gaagttaaaa agccagaaac tataaattac cgtactttaa agccagaaaa agatggtctt
     9121 ttctgtgaaa ggatatttgg accacaaaaa gactgggagt gtcattgtgg taaatataga
     9181 agagttagat ataaaggtgt agtttgtgat agatgtggag tagaagtaac taaatcaaaa
     9241 gtaagaagag agagaatggg acatatagag ctagctgctc ctatgtctca catctggtac
     9301 ttcaaaggta taccaagtag aatgggactt ttacttgata tgtcaccaag atcgttagaa
     9361 aaaatactat atttcgcctc atatgtagta gttgatccag gtgaaactgg attgaatgaa
     9421 aaacagttac ttacagaaaa agaatacaga actgctcttg aaaagtatgg atatactttt
     9481 actgtgggta tgggtgctga agctgtaaaa acattactac aaaatataga tttagaacaa
     9541 caaagtaaag acttaagagc ggaattaaaa gatagtacag gacaaaagaa agtaagaaca
     9601 attagaagat tagaagttgt agaagcattt aaaaaatctg gaaataaacc agaatggatg
     9661 attttagatg caataccagt aataccgcca gatttaagac caatggtaca acttgatggt
     9721 ggaagatttg caacttcaga cctaaatgat ttatacagaa gagttataaa tagaaataat
     9781 agacttaaaa gactattaga gcttggagct ccagatataa ttgtaagaaa tgaaaaaaga
     9841 atgcttcaag aagctgttga tgcattgata gataatggta gaagaggtag acctgtaaca
     9901 ggacctggaa atagaccact taaatcttta tcagatatgt taaaaggtaa gcaaggtcgt
     9961 ttccgtcaaa acttacttgg taagcgtgtt gactactcag gacgttctgt tatagttgtt
    10021 ggaccagaac ttaaatttta tcaatgtgga cttccaaaga agatggcatt agaattattc
    10081 aagccatttg ttatggataa attagttaaa gaaggttatg cacataatat aaaaagtgcg
    10141 aaatctatag tagaaaaagt taagccagaa gtttgggatg ttttagaaga tgttataaaa
    10201 agtcatccag ttcttcttaa ccgtgcaccg actctacata gattaggtat acaagcattt
    10261 gaaccaatct tagttgaagg taaggctata aaactacatc ctcttgtatg tacagcttat
    10321 aatgcagact ttgatggtga ccaaatggca gtccatgtac ctttatcagt agaagcacaa
    10381 gcagaagcaa gattcttaat gctttctgta aataatatac ttgctcctaa agatggttca
    10441 cctataacta ctccatctca ggatatggtt ttaggttgct attatctaac aatagaagcc
    10501 caagatggag ctaaaggaac aggtatggta tttaaagact ttaatgaact attacttgct
    10561 tattacaata aatcagttca tctacatgca ttagtgaaac tgaaggtaac acttgaagat
    10621 ggaagaagtt cattggttga aagtactgtt ggtagattta tatttaatga aaatatacct
    10681 caagacttag gttttgtaga tagaaaagaa aatccatttg cacttgaagt tgatttctta
    10741 gcagataaaa aatctcttgg taaaataata gataaatgct ttagaaaaca cggaaataca
    10801 gaaacagctg aattactaga ttatataaaa gctctaggat ttaaatattc tacattaggt
    10861 ggtataacag ttgctgttga tgatatgagt gtcccagaag aaaagaaagt atttatagct
    10921 gaggcagaag ctaaagttga taagtatgaa aaagcataca gaagaggtct aatctctgat
    10981 gaagaaagat atgaaaaagt tatagagaca tggacagaaa caactgataa agttactgat
    11041 gctcttatgg gtggactaga tagattaaat aatatatata taatggcaca ttcaggagcc
    11101 agaggttcta aaaaccaaat tagacagctg gcaggtatgc gtggtcttat ggcaaatgca
    11161 tctggtaaaa cagtcgagat accagttaaa tctaatttcc gtgaaggttt atcagtacta
    11221 gaatacttta catcttcaca tggtgccaga aaaggtcttg ccgatacagc tatacgtaca
    11281 gctgaatctg gatatttaac aagaagactt gttgatgtaa gtcaagatgt tattgtaagg
    11341 gaaatagatt gtggaacaga agatactaca gagatttatg ctataaaaga aggaaatgaa
    11401 gttatagaag agatatatga tagaattgta ggaagatata ctatagaccc tatattaaat
    11461 cctgaaactg gagaagttct agttgaggct gattcaatga tacaagaaga tgaagcagaa
    11521 actatagtag ctttagggat tgaaaaaatt aggataagaa cagttcttaa ctgtaaaact
    11581 aatcatggag tttgttctaa gtgttatggt agaaacttgg caacaggaaa agaagttaat
    11641 ataggtgaag cagttggtat aatagcagct caatctatcg gtgagccggg tactcagctt
    11701 acaatgcgta cattccatac tggaggagtt gcaggagctg atataactca aggtctacca
    11761 agggttgaag aattatttga agctagaaaa ccaaaaggat tagctgtaat aactgaagta
    11821 tctggtagag ttgaaattga tgaaacagga aagagaaaag aagtaaatgt aataccagaa
    11881 gaaggcgaga ctcaaacata tgtaattcca tatggttcta gattaaaagt taagcaaggt
    11941 caaatgctag aggctggaga ccctctaaca caaggattta taaatcctca tgatatagta
    12001 agagtaaatg gtgttaaggg agttcaagaa tatatagtta aagaagttca aagagtgtat
    12061 agacttcaag gggttgatgt taatgataag catatagaag ttatagtaag acaaatgcta
    12121 tctaaagtaa aagttgaaga cccaggagat acagatttat tgccaggtgg atatgaagat
    12181 gtattaacat tcaatgaatg taataaagat gctatagata aaggtctaag accagcagtt
    12241 gctaaaagag ttttacttgg tataactaaa gcatctcttg caactgattc attcttatca
    12301 gcagcttctt tccaagaaac aacaagagta ttaacagagg cagcaataaa aggtaaagaa
    12361 gaccacttaa taggacttaa agaaaatgtt atattaggta agttaatacc agcaggaaca
    12421 ggaatgaaga aatatagaaa tatagctgtt gaaaaaattg aagattaata gcatatagag
    12481 taaagaaatc ttgacaacca ttatttgtgg tgttaaaata gtaaagtgtg ttgtttaaaa
    12541 gtacactaag ggcttgaaca agttcttagt gacttttaga tggcaataaa tgagaactaa
    12601 aaagcatcaa gctagaaaag tgctaatatc acatatttat gtgttataga cattaaattg
    12661 taagtaacga atgttacaat cagtcagtat gaatattcat attgatgtag ttggcggtca
    12721 ctgataagat cgaaaatttg gaaggaggtg cagatgatgc caacaattaa ccaattagtt
    12781 cgtaaaagta gaaaagcgct agagaagaaa tctactgctc cagcattaca aaaaggttac
    12841 aactctttaa ataagaaagt aactgacgct agtgccccac aaaaaagagg ggtttgtact
    12901 tcagtaaaaa cagttactcc taagaagcct aactcagctt taagaaaagt tgccagagtt
    12961 agattaacta atggtataga agtttctgct tatataccag gagaaggaca caacttacaa
    13021 gagcatagtg ttgttcttat aagaggagga agagtaaaag accttccagg ggttagatac
    13081 catatactaa gaggtactct agatacagca ggtgttgata agagaagaca atcaagatct
    13141 aaatacggtg cgaagagacc taaagaagct aaaaaataaa acaattaatg attgattata
    13201 gttcggtgct ttaaatatat atatatttac ggcatcacgg cattaaatga tttatgtcga
    13261 gtacctatga tttaagttaa ataattaagg agggaagcga aatgccaaga aaaggtaata
    13321 ttccaaaaag agaagtttta ccagatccta tgtatgggag taaggtagtt actaaattaa
    13381 taaataatct aatggtagac ggaaaaaaag gaaagtctca aagaatagtt tatgatgctt
    13441 tcgcaattgt tgctgaaaag acaggagaag aagctttaga agtatttaat aaagcaatgg
    13501 ataatataat gcctgtttta gaagtaaaag caagaagagt tggtggggca aattaccaag
    13561 tgccaatcga agttagacct gaaagaagac aaacattagg tttaagatgg ctagtaaaat
    13621 atactagagc tcgtggagaa aaaggaatgg ttgaaaagct agctaaagag ataatggacg
    13681 cagctaacaa cactggagct tcagttaaga aaaaagaaga tactcataaa atggcagaag
    13741 ctaataaagc atttgctcat tacagatggt aggatatata gatatcttat aaattcaagg
    13801 atatcataag gaggataacc atggctagaa agtttccttt ggaaaaaact aggaatatag
    13861 gaatcatggc tcatatagat gcaggaaaga ctactactac agaaagaatc ctgttttata
    13921 ctgggcaaac tcataaaata ggagaaactc atgaaggagc ttctcaaatg gactggatgg
    13981 agcaagaaaa ggaaagaggt ataacaataa cttctgccgc tactactgct tcatggaaag
    14041 accatagaat aaacataata gatactcctg gacacgtgga cttcacagtt gaggttgaaa
    14101 gatctctaag agttcttgat ggttcagttg ctgtattctg tgcaaaaggt ggggttgagc
    14161 ctcaatctga aaatgtatgg agacaagctg aaacttatgg agtacctaga atagctttta
    14221 taaacaaaat ggatatatta ggtgcagatt tctacaatgt tataagcatg atgaaatcta
    14281 gattaaattc taatgcagtt cctatgcaat taccaatagg taaagaagat tcatttatag
    14341 gtataataga cttattaaaa atggatgctg ttatatacaa agacgaccta ggggttgaaa
    14401 tggaagaaac agatatacca gaagatatga aagaattagc tgctgagtgg agagaaaaat
    14461 tagttgaatc agtagctgaa actgatgaag aattaatgat gaaatacctt gaaggtgaag
    14521 aacttactat agatgaatta aaagttgcta taagaaaagc tactatagct tgtgaaatga
    14581 acccagtttt ctgtggtaca gcttatagaa acaaaggtgt tcagttagta atagatgcag
    14641 ttttagatta cttaccagca ccaactgata taccagctat aaaaggtata ttagcagatg
    14701 gagaagaagc tgaaagacat tcttctgatg aagagccatt ctctgcatta gcattcaaaa
    14761 taatgactga cccattcgtt ggaaaactag cgttcttcag agtttattct ggaacattag
    14821 aatcaggttc ttatgtttta aatgcaacta agaataagag agaaagaatt ggtcgtatat
    14881 tacaaatgca tgctaataca agagaagaaa taactaaagt gtatgctgga gatatagcag
    14941 cagcagtagg attaaaagat actactactg gagacacatt atgtgaccct gctaatccaa
    15001 taatacttga atctatggaa ttccctgagc cagttatatc tgttgctata gaaccaagtt
    15061 ctaaagcagc tcaagaaaaa atgggtatag ctcttcaaaa attagctgaa gaagacccaa
    15121 ctttcacagt taagactgac caagaaacag gacaaactat aatatctggt atgggtgaat
    15181 tacacttaga gataatagta gatagattat taagagaatt taaagtagaa gctaaagttg
    15241 gtgcaccaca agttgcatat agagagacta taactcaacc agttgatgtt gaatacaagt
    15301 actctaagca atcaggtggt agaggacaat acggtcacgt taagattaga gttgctcctc
    15361 aagagccagg tgaaggatac aagttcacaa acaaaactgt tggtggatca gtaccaaaag
    15421 aatatgttgg tccagttgac atgggtatac aaggagctat gcaatctggt atagttgctg
    15481 gatatccagt tgtagacgtt gctgttgagt tatatgatgg ttcttaccat gaggttgact
    15541 cttctgagat ggcgtttaag atggctggtt caatggcatt caaagatgct atgaagaaag
    15601 gtaatgcagt tcttttagag ccttacttca aagtagaagt tgttactcca gaagattaca
    15661 tgggagatgt tatgggtgac ttaaactcaa gaagaggatt aatccaaggt atggaagcta
    15721 gaagtggtgc acaagttata aatgcattcg ttccactttc agaaatgttt ggatactcaa
    15781 cagacctaag atcttcaact caaggtcgtg caacttacac aatgatattc gatcactatg
    15841 agcaagttcc agcttcagtt gctaagaaaa tagctgaagg aaaataattt aaaaataatt
    15901 aataatataa atataaaaaa taatcagcta gatgagatat atctcatcta gcacaagat
//
LOCUS       DAGQWR010000099        15717 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.099, whole genome shotgun sequence.
ACCESSION   DAGQWR010000099 DAGQWR010000000
VERSION     DAGQWR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.099"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(22..882)
                     /locus_tag="MIW95_002692"
     CDS             complement(22..882)
                     /locus_tag="MIW95_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBY3152004.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1199..2527)
                     /locus_tag="MIW95_002693"
     CDS             complement(1199..2527)
                     /locus_tag="MIW95_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBY3152005.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2675..3004)
                     /locus_tag="MIW95_002694"
     CDS             complement(2675..3004)
                     /locus_tag="MIW95_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY3152006.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3028..4317)
                     /locus_tag="MIW95_002695"
     CDS             complement(3028..4317)
                     /locus_tag="MIW95_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY3152007.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4333..4659)
                     /locus_tag="MIW95_002696"
     CDS             complement(4333..4659)
                     /locus_tag="MIW95_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3152008.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4794..5423)
                     /locus_tag="MIW95_002697"
     CDS             complement(4794..5423)
                     /locus_tag="MIW95_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152009.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5638..6654)
                     /locus_tag="MIW95_002698"
     CDS             complement(5638..6654)
                     /locus_tag="MIW95_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY3152010.1"
                     /translation="MVKAVGDIIGKCSAISFDMKLDKSYDEVIVDFKNLINNIDNKDV
                     LLFTDMGSLNSFDEIIKKEKKCEVRVIPMVTTLTVLEAVQKANMGLPLNDVYNSITNT
                     RKYYFGTNEIQNKENLSKTIIIASHVSEGVDNKTRKILEEKMSRYLDGIDIISVPYKT
                     EKDLSLNITKLKESSNIVAVINEQRINIRGIDYISKKDIDKDENINKLKNIIKISIGY
                     DDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLVFNIEKKYDKVIGLMMHLAFMVDGLI
                     GNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDKKYNIEINEKECYQILLILEYAEIIE
                     KDYQ"
     gene            complement(6635..8293)
                     /locus_tag="MIW95_002699"
     CDS             complement(6635..8293)
                     /locus_tag="MIW95_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3152011.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERDSKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASNGKSSW"
     gene            complement(8652..9596)
                     /locus_tag="MIW95_002700"
     CDS             complement(8652..9596)
                     /locus_tag="MIW95_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152012.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9589..10614)
                     /locus_tag="MIW95_002701"
     CDS             complement(9589..10614)
                     /locus_tag="MIW95_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152013.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDEYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10689..12257)
                     /locus_tag="MIW95_002702"
     CDS             complement(10689..12257)
                     /locus_tag="MIW95_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY3152014.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHVLEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12370..13281)
                     /locus_tag="MIW95_002703"
     CDS             complement(12370..13281)
                     /locus_tag="MIW95_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152015.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13265..14251)
                     /locus_tag="MIW95_002704"
     CDS             complement(13265..14251)
                     /locus_tag="MIW95_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152016.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLNEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14610..15011
                     /locus_tag="MIW95_002705"
     CDS             14610..15011
                     /locus_tag="MIW95_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBY3152017.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLVAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15014..15412
                     /locus_tag="MIW95_002706"
     CDS             15014..15412
                     /locus_tag="MIW95_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBY3152018.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMVG"
BASE COUNT         4940 a         2736 c         1736 g         6305 t
ORIGIN      
        1 ttaggattct gttacataat attatttaaa tattcttcta tatttctctt cttcttcttc
       61 agtaaacaca ttaaatacaa cttttaaatt tttttcagga ttttctttta gccatagtct
      121 aactctactt acagcaagat tagctgcctc tttttttgga taaccaaata cacctgtaga
      181 aattccacaa aaaacaatat tttttatttc atcaatttct tttattgtat ttaaacatga
      241 tttataacaa tgaagcagtt gcttttcttg ctctttactt ggctgaccac cagatacaat
      301 aggtccaaca gtatgtacta caaattttgc aggtaaacaa tagcctctag ttatcttagc
      361 atctccagta tattctaaat gcccttgttt ttttattatt ttgtcacaat cttctctaag
      421 tctaggacct gcgcatgaat gtatctcatt atccacgcat agatgtaatg gttgtaaaca
      481 ccctaataac ttgttatttg cagcatttac tattgcatct gctcttaaat tcgttatatt
      541 acctctccat atggcaatcc cttcctctac ttcttctata tcatttacat ctactaactc
      601 tctttcttca cattcaatca acaataaatt atgtaacata ttaagtatct ctggagcaat
      661 ttcttttggt ggatatacat tcaaaagtcc tctaagtata tctctaggct taatttcatc
      721 atttgataaa ttgtatattt tagaaggaac ttcttttgaa aaatatgcaa ttaaagtgtt
      781 tatatttttt cttctttgtt cgtcagttag aggcttgata gttttatcaa aatcctcaaa
      841 taaatttaca tcatcagcat aatctctcca cttcatagcc atctctccat tcttgtattt
      901 tagacttaaa aataattatg tatttatgta catatttatt tatttctata aacacctaca
      961 taaatcctgt taaaagtgta aattttttat tatgcaactt ctctggttat ttctattgat
     1021 tgatagtaaa ttattgtacc taaatttaat ccaactaaca agtaactaaa cctactttcc
     1081 taactgcaaa atacactatg tttgccactt gtatctcatt tgtaaataaa taaagtccct
     1141 acaaatacat taactaggct atgtactaaa ctgtaggaac tttaaaaata tattattctt
     1201 aattaagttt tggccaataa tttttatttg cttctattaa atcatctagt aattcttttg
     1261 caactgttgc actaggaact gtcttagata gcataagtgc ttgccacaat ttattataag
     1321 acttctcaat ccaagcatca actactaatt tttcaactgc taattgttgt tctattaaac
     1381 ctttttggaa tgttggtatt ttccctattg ataatggttc atacccgtct tttccaacta
     1441 tacatggaat ttcaaccata gcatcatctt gtatattttc tatagctcca ttattttgaa
     1501 ctatcaataa catacgctcc tgtgtattaa aagctagagc tgtagctaaa tccactataa
     1561 actctgcatg tattcctatc ttaagcccac tgcccttaga atgtccttgc ttttctatca
     1621 atgcacatga accaaaaact tctttttctc ttccatcaat aacttcattg gcacgagtat
     1681 aatttacatc agaatgattt acaacataat ctggaaatag atagtacttt aagtaactac
     1741 ttggtattgt agttaaatct gtttttacta tttctttagt aaataaatta gtttcaaacc
     1801 aactatcttt cttgtcattt ttcattcctt cttcaccatt tataaaacca tattctttta
     1861 tatgttctac taattttggt aaaagttcat ttcctttttt atctttaatt gaagtatacc
     1921 aaccaaagtg atttaatcca aagtatctta tatccatatg tttacgagat ttgagtcctg
     1981 caatccttgc tatattatgt tccataccta ttggcatatc acaaatattt attaccctag
     2041 aattaggtct caaccttcta acagcttccg ctattattga tgctggatta gaataattta
     2101 acatccaaca atttggagaa tacttttcca tataatctat gttttcaaga acaccttcta
     2161 tagacctcat tccataagct attccacctg gtccacaagt ttcttgacca actactccat
     2221 gttttagagg tatcttttca tctagttctc tcatttcaag ttttccaact cttatatgag
     2281 ctaaacaaaa atctacatct gtataagcct ctttagggca tgttgttgct aaatactcta
     2341 tattagggtc cttttccttt attagtattt cacaagcttt agctacctta ttttgtcttt
     2401 cttcatcatt atcatataat ttgattgctc ttaatggtaa cctatctaag ttatctagca
     2461 acattaatat gatttctggg gtatatgtac ttcctcctcc agctattact actgaaaatt
     2521 tttccataat aatatctcct tttctattta aaaatttact gtatttataa ataaatttat
     2581 aaatactagt actttaaagt aaaagggagt tcatttatta aatctattga tttaataaac
     2641 tatagccaaa cttcaagcac tatttacaag aaatttaatt cttattaatt cttgggtcca
     2701 tcatctcaaa tatatctaca agttcagata ctaaagaatg agcaaataat gcatttgaat
     2761 aatggtcttc agagtgcaca actaaaaaat cagtcccact atcttcacta ttcatagcat
     2821 ttgatagtaa ttctaaatga tgctttgatg ctgtttgtat tttctctgag gctgaattta
     2881 aaaggtgttt tgctttattg tagtcacctt tttttatttc cttaagagac atcattacat
     2941 cacttttagc atctcctgct tctgatatta tactaaatac tttttcttcc atttcaggta
     3001 ccatatcctt ctccttctaa tgacttgtta aaaatcaaag ctatcatcat ctaatgttgt
     3061 agctaattct gcatttttat ttaattcttc cttctccatt atcttaaaga atggataata
     3121 tatcaaggtc ataagtatta tacttgcaaa tactaatacg attgccttcc aatctaatgt
     3181 catcaaaaat gctgcaaccc ctggaggtaa aaagcctggt ggttctgcaa taggtaatcc
     3241 tactaatcct acttttaatg ccataaaatt aagtgctcca accaatacag gtccaaatac
     3301 aaatggtaca aacatatatg ggttcataac tattggtgtt ccaaatatta ctggctcatt
     3361 tattccaaat agtgatggta aaatcacaac ttttcccata ctattaaaac tcttacactt
     3421 acttcttgac ataaggtaca ctaatcccaa agttatccct gaaccagtta cttgcaagaa
     3481 accatagtaa aatcctattg taaacacata ttttgggtct cctcctgctg ctatcagtgt
     3541 tgcattttct gccatatatg tagctgctat tggcatccat atacttgaag ttattgctgc
     3601 tccatgaagg ccaaagaacc ataataattg agttataaat agtattatag ttactgcaaa
     3661 aacattatct acactgctta tagctggtgc taagaaattc attataatag ctggtaatgg
     3721 ttgacctgag acatttttag ttattgctgc tattgctgta aataacatta tacatactat
     3781 cattggagtt aaatttctaa aacttttatt tactacacta ggtacttctt ttggaaatct
     3841 aattacgaga tttttatcat tcataaactt agtaatatca actactaaaa gtgctactaa
     3901 tatacttaca aataaacctt gtgcatctaa ataacttgta tctaatacac catctactac
     3961 ttgtccacat acaacaagtt gtgcaacaac tgctgtcata cctatttcta catgattaag
     4021 attatatttt ttagctagtg cctgagctat aaaaaatgct gaatatatag ataataaact
     4081 catagtaaat ttattaggga taagtaatgt atccatattt tcagataata atgtttttgc
     4141 acttccttca ggtaacatat tgtttaatgc cataaataac atacttaaag aaccaacaat
     4201 tattattggg gtaatgctta tcataccttc ttttatagct tgaagatgcc tttgtttttc
     4261 aatcttttgt gctattggta gaagaaatct tcttaaatta ttttgaagtg cttccatttt
     4321 tattcccccc aattatctat ttttatataa ttcgattgcc gacttcataa cagaaggagc
     4381 atccatacga ccataatgct tagtatctat taaaccaact gctattttgt aattcttaca
     4441 agcttcaact acatcatctt ccttgaacct aatttgtggt gctacaagta ctacatctgt
     4501 atcattagct tcaatgtatg attttaaatt ttcgaatgat gttgcctcta tatttatagt
     4561 tttagttttt aacttttcat gctcactaca aaaacttttc atcttttcca caagtacacc
     4621 tgttgaagct ccatttgcac atactaataa aatattcata attaccctcc tacaatatct
     4681 aacctctttt attttctaat tttttatcag ccaattctta ctctaattcc tatcagtttt
     4741 tactttaatt cttattattt tattttaatt cttattaata tttactctaa ttcttagtac
     4801 tgccaatgat taaacttgaa agccgtatct ataacttctt cttgcttata tttttctaat
     4861 atttttaagt atctttcttt ataatcatta tctacttctt tttctggtat tactatacta
     4921 gcttcattta aaaagtggaa tgaatccctt cccatttcta atcctctata tgtatgcttg
     4981 tcaagtgcca cgtccataac ttctggaact ttacccattg caaatccttt tataattata
     5041 ttttttaata aatcacttga tctatctttc tcacatgcac aaagatatct tattgcatgt
     5101 atgaaaaata aaggtctgtc tccatctgca tatgaatatt cctttcttac ttgatttaaa
     5161 ttatttatga ttattacagc tgatgggtcc cccattccta tatcttcaac tgatattgta
     5221 agtatccttc tccatagctt atcttctaat tctggtgatg atatatatag ttcataagca
     5281 aattcacaag cttcttccac catatttctt cttatagctt tttgaaatga cgatattatt
     5341 tcgtctgcac taaatccgtt tcttgtagtc attcttgccc atgggtcatt tgccttttta
     5401 tctcttaata ttttatcttg catattattt actccttttt atttaaattt taattattta
     5461 tattactcat ttaataatat aagcaagtat tatgccagtt tttttagaca ctttaaaaca
     5521 ctgttatttc aatcattttt atcatcaaaa cttagatact ttgtctaagt atatttatat
     5581 gtgtctaata ttaatattat tttagactaa aaaaaattac caaataagtt tttatactca
     5641 ttggtaatct ttttcgatta tttctgcata ttctaatatt aataatattt gatagcactc
     5701 tttttcatta atttctatat tatatttttt atctaattga gatactatat cttttatttt
     5761 gcttaggctc ttgtgataat caagtgtttt ttctttatca agcttttcaa tttctctagt
     5821 atttccaatc aacccatcta ccatgaaagc taaatgcatc ataagtccaa ttactttatc
     5881 gtatttcttt tctatattaa atactaaaaa caattcatca gacacatatt ttatatcttt
     5941 aaatatccta ttataattag agctttttaa ggatgtcttt aaaccttcaa ctacatcatc
     6001 ataacctata cttatcttta taatattttt taatttattg atattttcat ccttatctat
     6061 atcttttttg ctaatatagt caattcctct aatattaatt ctttgttcat tgattactgc
     6121 aacaatatta ctactttctt taagttttgt aatatttaaa cttaaatctt tttctgtttt
     6181 atatgggaca gatataatat ctattccatc taagtaccta ctcatctttt cttctaatat
     6241 ttttcttgtt ttattatcta caccttctga aacatgtgat gctataataa ttgttttaga
     6301 cagattttct ttattttgaa tttcattagt tccaaaataa tactttctcg tgttagtgat
     6361 agagttgtat acgtcattta aaggtaatcc catattggcc ttctgaactg cttctaacac
     6421 agttaatgtc gtcaccatag gaattactct tacttcacac tttttttctt tctttataat
     6481 ttcatcaaag gagtttaatg aacccatatc tgtaaatagt aatacatctt tattgtctat
     6541 attattaatt aaatttttaa aatctacaat aacttcatca tagcttttat ctaacttcat
     6601 atcgaaagaa atagcactgc actttccaat aatatcacca actgctttta ccattacttg
     6661 ctgtagtttc tccatgcata gcaactataa tattaatgtt attcactatt ttattttgtt
     6721 catcttgttt agcttgtact atgaataatg taatatatgc tacagtttct atagataaaa
     6781 ataaatcaaa ttcattttca attattccaa ctatttttag tgctgtttta aattcacttc
     6841 tgttatttct tctaatttga ttcaaatcta tgttgtttgt agagctactt ttatccaata
     6901 cgctttttat ataaattgcc attgacttaa atattgattc acttatatct atttcttcat
     6961 cttctttaat aatttgtctt attgattcac acatgctttt aaacttaaca ttattaaata
     7021 gagaagcatc tgctatattc atttctacag atttattaag attagtattt actgcattaa
     7081 taatatttat atcattgtag ttaataactt cttcttctcc taatgacgta aatacaaaat
     7141 attctattcc atgtgttata acttcatctg ctttaataga cttctctcta taatcatcac
     7201 tcacttcatt tatagattta tcaaaatatt ttttctctat ataaactggt tctttattat
     7261 ctcttttata tcttaggtat ccatttgcaa cagctaattt tatatcattt tgaagttgac
     7321 ctacattatt tttacaatca taaagtaata gtgatttaat tatttcttta tgtactttta
     7381 ttgaaataga aatacttttt gattcttcct tgaaaaaatt tttgactaat tccattcttt
     7441 caatcattgt cctatctctc agtgatggta tttctatttt cattggtatt cttctattga
     7501 atgtatctaa tagtgttgat gtcggatttt ctgtagtagc acatattatt aataccttag
     7561 aatctctttc tttcgttgca tctcctagtc ttttaaatgt tcccttatct ataaaagtaa
     7621 acaacatttc ttgaccttct gctggtagtc tatgaatttc atctaaaaat agtatccctc
     7681 catttgcaat ttccaaaaga ccttttctgg aagattctgc acctgtataa gctccttgtt
     7741 ctactccaaa aatatgtccc ataagtaatt gtgcattatt agcataatca gcacaattaa
     7801 atgttacaaa aggagcttta ttttttctaa ttccactatc tatagaataa tcgtacatat
     7861 atctagcaat catactcttt cctactcctg tttctccata gataatagta tgaagtccat
     7921 ttgtaggata gagcattgcg accttgcatt tttctattat aggtcttaaa ctatgttctt
     7981 tacctattag tgtttcaaaa ctatcttttt ttattttgtt ttctactttt gttccttttt
     8041 ttatgccaag caactcactt tgtgtatcaa gcaattcatt cttgttaagg ttaagatttg
     8101 cagggatata atacacaggt cttcctttta tcttcgtaac cataccctca ttgaataatt
     8161 ggtttaaata tgtacttata ttgtttcttg caattcctgt cttttctgat aattggttag
     8221 ctgtaatccc tttttcattt tccctatatg atgattctaa aactttcatt actttatctt
     8281 ttttatatat cattacaagc tcccctacat tataatattt tattaactaa aacccctata
     8341 cacactttat tttttaataa actcttattc tttaactaag gttataattt atcaaacttt
     8401 ccttacttta aattatagaa aattattgta gaaaaaataa gattattgct cattttttct
     8461 acattactat ttttaatttt atagcacttt cttaacaaca tactttactt gtttttaatt
     8521 ttattatatt tgaatttatt atatttgaaa agtagaaata aaaaacccta ttttggagta
     8581 gttttaaaat ttctccaaaa atagggtttt agtttgtatt tcaaataaat acctgttcaa
     8641 gtatttttat actaaacaac ttttaaattt tgttgatatt tatttatttc tgatttagaa
     8701 gaatatacaa aatgtccatc tgaaacctca atccaactta atgacttttc atttgaacaa
     8761 tcatatccat tagaattata ctctattcta tttcttgact tagcatatct agggtcagga
     8821 acaggaattg cagaaagtag tgattttgta tatggatgaa taggattatt atacaattca
     8881 tttgaatctg ccaattctac taattttcca ttatacataa ctcctatacg gtcactaata
     8941 tgtctcacca tagataaatc atgagctata aataaacaag ttaaattctt ttcttgttgc
     9001 aattttttaa ataaatttac tatttgtgct tgtatagaca catccaaaga agctataggc
     9061 tcatcagcta ttataaattc tgggtctact gaaagagctc gtgctatgcc aatacgctgt
     9121 ctttgtccac ctgaaaaatc agatggatac ttatttgcat aacttctatc aagtcctact
     9181 agctttagca tctcatatac tttattcatt ctatctgttt tatttttaca gataccttga
     9241 atttttagtg gctctgaaat tatatctgca attgtcatac gtggatttaa tgatgaagta
     9301 gaatcttgaa aaataatctg catacttttg tttacatctt tttttataaa tttgtacgtt
     9361 tttttatctg atatgcaatt tcctttgtaa attacttctc caccagtaga ttcatgtagt
     9421 cttattatag ttttaccagt tgtagatttt cctgaacccg actctcctac caatccaaat
     9481 atctcaccct tatatatatc aaaacttata tcatcaactg cttttacagt agtacttttg
     9541 tccaaatgaa aatattgctt taagtttttt atttccagta ttttttctct attcattaga
     9601 aatcaccctt ccacaattta ctctaactgg tacatctact tcaggtgcat ctgggtgcaa
     9661 taaccaagtt gctgcactat gtgtatcatt aattttaaac attggtggct ctttttcata
     9721 atctatctta agtgcatttt tatttcttat tgcaaaagca tctccttttg gtgggttcaa
     9781 taaatttgga ggcattccag gtatattata taaatattca tcctgtgaat ctaaagtagg
     9841 taatgaaccc aacaaccccc aagtatatgg atgtctaggg tcataaaata tatcctctac
     9901 agttcctatt tctacaatct ttcctgcata cataactgct attctatcag ctatagttgc
     9961 aactactcct aaatcatgag ttataaatat tattgacaaa cctattttat gttgtaaatc
    10021 ctttattaaa tcaattattt gagcttgtat agttacatct aaagcagttg taggttcatc
    10081 agcaatcaat acatctggat tacaggcaag tgctatagct attactatac gttgtctcat
    10141 tccacctgaa aaatgatgtg gaaattgttt aaatcttttc tcaggattat caattccaac
    10201 caattcaata agctctatgg ctctcttttt tgcttcactt ttacttatac cttgatgaat
    10261 gattacagct tctgcaattt gctttcctat tgatatagtt ggattgagag atgtcatagg
    10321 gtcttgaaat atcattgata tattctttcc tcttatatct tccatctctt tttcagactt
    10381 cttaaccaaa tcacttgatt taagtaaaac ctctccattt ttaatatatc cattgtatgg
    10441 gagtatcctc atcaaactct tacacagtac agatttccca caaccagatt ctccaactat
    10501 agccaatgtc tcaccttttt ttagattaaa agatacattt cttacagcct gaacttctcc
    10561 ttcttctact ttaaaactta cagatagatt attaacctcc aataaatgtt ccaaaaacca
    10621 tcaaccccta tgctattcaa accttaggga tatattttta tccctaaggt ttctaattaa
    10681 tttcagtatt attcaattgt ccagtcagct atgttccaga atatacctac accatgatga
    10741 cctaaaactg tatcaggtgt taatcctttt atgtttggtt ttccaacata tattgcatct
    10801 atataagcaa taaatgtata aggcatatcc ttagtcattt ctacttggaa ttgtttatat
    10861 aactttaatt tttcatcttt atcttctgtt tctcttgctt tttgaagtat tttatctata
    10921 gtaggattag agtaagcact gtaatttgca cctttatctg ttccaaatac tttatatgta
    10981 tggtcatctg ggtcaaatgg acttccccaa cctattaaat gagcatcttg attagcccaa
    11041 tctgtttcag ttacaacaac agctttagca tcaacaccaa tttcttttaa ctgttgtgca
    11101 catattttag ccatatcaac tctaacttgg tcactctcac ctgctgttat ctcgaaagct
    11161 aacttagtac cttctttttc atagattcca tcagaaccta atttccaacc tagcttttca
    11221 atttcttgtt tagctttttc tggattatat tcaaactttt ctatatcagg attattatat
    11281 ggtcccattt gtaaaggtga atatgctggt acaccatgtc ctaataatac actgtcaact
    11341 attgcttttc tatctattgc atagcttaaa gcatttggta atcctttagc ttttttatct
    11401 ttaaagaatt tagaattaaa attgtataat atacctctat agtcggctgt tttcattata
    11461 tttactttaa aattcttttc gtctttttca aagttagaca tatctttagg agttatttga
    11521 gctaagtcta attctcctga ttttaattgc attgccttag ccttatcatc tggaacaatc
    11581 ttaaagacta ctttatctaa tcctggttct tttacaaaat aatcactatt cttaacaagt
    11641 gttatacttt gacctttgtc ccatttttca agtttaaatg gtccagtacc tattggtttt
    11701 tgattaaatt catcagtagc tatatcctta ccttccaaaa catgttttgg taatactcct
    11761 actgttaaat aatcaagcat tgctgtattt ggagctttta aagtaatttt tatagtatta
    11821 tcattaacca catcaatttt tgttatatct tcataatttg atgctatctc agaagcatta
    11881 tctggattca ttatagtttc tattgtaaac ttaacatcat ttgccgtaaa tttctctcca
    11941 tcatgccact taacatcatc tcttaaatta aaagtatatg tatttgttgc ctcatcaaat
    12001 ttccagtcct ttgctagaca aggaaccacc ttattatttt catcatgagc tgttaatcca
    12061 ttaaatatta atgaatttat ttctccatgt tcatatagtg caggatttat acttgtatag
    12121 tcattacttc cataaacaag tacctttcca tctttaggcg tatctgcctt cttatctttg
    12181 tctcctccac tagaacaacc tgccattaga ctaagtatca ttactaaact taaaactttt
    12241 aacttcttta atttcatgat gctccccctt ttgcttttta taaaatattt ttcaaagctt
    12301 tgaccccatt tatttaaata gatatttcaa atacctcagt atttgcttat ttagtactca
    12361 aatgctgatt tataaattac tagatttttt attattattc tttcttatat aattacctat
    12421 atttgttata cacaccaatg tggtaaccaa aaatataccc ggaatcagta taatccacca
    12481 tctatttgaa agtaatgctt cctctgacaa agataacata cttccccaag aaacaatttc
    12541 tattggtaaa ccaattccta aaaagctaag tgtcgattca gtaccaatag ctgcgcctat
    12601 gtttgttaca accatgaaca ttattgatgc cacaaaattt ggaagtaaat gttgctttaa
    12661 tacatagaag aatcctcctc ccatgctctt agctgctagt atgtattcgc tatttcttat
    12721 ttgtctaact tctgttctca ctatcttaga tatattcatc caacttgtta tccctattac
    12781 tatggacata gatactggat ttgaatttcc aagtatagct tgtacaaata ttataattaa
    12841 tattgaaggt atactaagta tgatttctgt aaacctcatc attgcatcat ctacgagttc
    12901 agatgctgaa ccacttatac taccataaac aattcctata acagttgata ttacagtgga
    12961 aaatagtcct ataaataaag atatttttcc tccataccat atcatagaat atatatctct
    13021 acccattgag tctgttccaa ataaaaaagt tttatttgga gctaaattag aacttgctaa
    13081 atccatgtat gttggttcat gtgtcattat aaaagatgag aatacacttc caatgactat
    13141 gattgataaa ataataattg atatatatgg taattcttta atttttttag ataaggtttt
    13201 ctttgtttct atatgttcat cttctgctac taaaatataa ttttctccta caatttcaaa
    13261 atcactatat tgtggcttca ttagtatcac ctctatcata tttcattcta gggtctattt
    13321 tattgtttat aatttgagct aacatattcc caaatactac taaagcccct gttattaagc
    13381 ataaaactaa taacatatta taatcatgat actttgcact ttcaaaacaa agtgttccta
    13441 acccaggata agaaaaaact ttttcaacta cataagttcc tcctagtata tgaggtatcg
    13501 aaatggccat tatactaata tatgatggca ttatatttct caggcaatgc ttaaaaacaa
    13561 tagttctatt atttaaacct tttgctttac ataaaagcac ataatcttct cttatttcct
    13621 ctagtaattt atttcttatc atatatgtgt aataccatag atggcttaat ataagtactg
    13681 taagaggtaa gattagatgt gatatcctac ttaatatact ggactcttct cccattgaat
    13741 aagcaccact acttggaagt atacttaagt ttatactaaa tataagtatg agtactaagg
    13801 caacccaaaa tgatggaata cagttggtta ttgttcctaa tttgcatatg attctatcaa
    13861 tgagcttatc ttcatgtaat gtacaaaata ttcctaacaa taaggctagc acaaaggtaa
    13921 gtatataccc tgaaccacct aatattatag tgtttatata gacatcattt ataacagaag
    13981 taacattttg tttatattta aatgaaattc caaattcccc ttttgatgca tttgaaatcc
    14041 attttatata ttgactgtat attggttcat tcaaaccaag ttttttcata gcattttctt
    14101 tttcttgtgt actcattctt tctactccat ccccatagta agagattaat gggtctcctg
    14161 gagcaagtct tgccatgtaa aaaacaataa aagataataa aaacattact aaaataaact
    14221 gcaacaactt tttcagtata tcctttaaca aaccccaacc cccctttgtt gcaataaaaa
    14281 aaccacataa aagcaaacgt aaagtaacgt taaaaccttc gtggttccat tatcattaaa
    14341 caaaattaaa ttttctatat ttatctatga gttaatttta gcatcattta ttaactgtgt
    14401 aaacaaactt tttaaaaatt gttaaaattt catcaaatta ttttatttat acctaaaata
    14461 aaatttttat gttctattct tcattatttt ctataaaatt ataaaatttt aggttgacta
    14521 tataagaaaa gtatattata ttagtttgta tgcaaaccat ttaagaggtg attttattga
    14581 aaagtaatta tgactgtttt agaatagcca tgctcttaaa agaactttat tctaaaacta
    14641 tgtacacagt agaagaaaat tttaaagaaa atggattaac acaccaacag attatagtta
    14701 taaaactggt agctcataat caagaactta caatatcaca actatgtgat gaaatgtctt
    14761 tagctaaagg tacggtttca ggcataatca gcagattaga acagattgga tatatagaaa
    14821 aattcaagaa atctaatgat aaaagaaata catacgttaa gtttacaaca acaggatttg
    14881 aatttgccac aaattttaaa attaaaatgc aagaaagttt tgatgatatt tttaaaaatt
    14941 gtgatgaaaa cgaattgagt gacttagtaa aaaatcttag aaatatattg gcaaaagtaa
    15001 aggagagata attatgaatt ataaattaat acttgcaata gtatttataa catcagcttt
    15061 aattttctac actataggtg tatttggaga aagaaaggca aaaattttaa aaaagaaaca
    15121 tgtaattata ttttggcttg ggtttatatt tgatacttta ggaacattta caatgagtaa
    15181 catagccaac agccatactt ttgaagtaaa atctgcttta tcacaaaacc ttcatagtat
    15241 tacaggtctt cttgcaattg tgcttatgct ttttcatgca agttgggcta cttttgtcct
    15301 atacaaagat gatgaggaaa agaaaaaatt ctttcataag tttagtatag ttgtatggac
    15361 tatatggtta gttccatact ttataggtat gtttattggt atggttggat agtaaaataa
    15421 aaagtgtgta agaaatactg taggtaaatt tgtataagac aaatttatct acatccttct
    15481 ttcacacttt tacatattta tctataatac tccattattt ctttcatgtt tcttaatacc
    15541 tatatagaat atttaattgt ttctatagta ttttattatt ttctcacact ttttcatata
    15601 tatattgttt tactattttt tcatatgtat atatgaaata ttccattatt tctataaatg
    15661 ttttattatc tttatcatac tttttcatat atatattgct tcactatttt ttcttat
//
LOCUS       DAGQWR010000100        15663 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.100, whole genome shotgun sequence.
ACCESSION   DAGQWR010000100 DAGQWR010000000
VERSION     DAGQWR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15663
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.100"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            78..620
                     /locus_tag="MIW95_002707"
     CDS             78..620
                     /locus_tag="MIW95_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY3152019.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            945..2438
                     /locus_tag="MIW95_002708"
     CDS             945..2438
                     /locus_tag="MIW95_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY3152020.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDCNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINKGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKVNELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            2675..3439
                     /locus_tag="MIW95_002709"
     CDS             2675..3439
                     /locus_tag="MIW95_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY3152021.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(3724..6903)
                     /locus_tag="MIW95_002710"
     CDS             complement(3724..6903)
                     /locus_tag="MIW95_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY3152022.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKRDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKETTIENKLTSSQFTKENNHYVFKGTDYDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIYKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIIDLFN"
     gene            7184..9361
                     /locus_tag="MIW95_002711"
     CDS             7184..9361
                     /locus_tag="MIW95_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY3152023.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALHSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANVMNIDDDLLKAGYEASIRHMFKKTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDNEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYRESDINSIIDDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            9375..9797
                     /locus_tag="MIW95_002712"
     CDS             9375..9797
                     /locus_tag="MIW95_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY3152024.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGACPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            10204..11007
                     /locus_tag="MIW95_002713"
     CDS             10204..11007
                     /locus_tag="MIW95_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBY3152025.1"
                     /translation="MKKLKLIIMLALVLALLITGCSSGNNKNKEEQKTIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMVEPPIQFGAC
                     SFAIQKNEKGEKWTKEINEVIEEMHKDGTLTKISEAWLGKDITKEPINE"
     gene            11000..11668
                     /locus_tag="MIW95_002714"
     CDS             11000..11668
                     /locus_tag="MIW95_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBY3152026.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMTEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     GKAY"
     gene            11904..12566
                     /locus_tag="MIW95_002715"
     CDS             11904..12566
                     /locus_tag="MIW95_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBY3152027.1"
                     /translation="MIFNMKYFLELFPIIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMNDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDKGQIEAAYVMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            12626..13423
                     /locus_tag="MIW95_002716"
     CDS             12626..13423
                     /locus_tag="MIW95_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY3152028.1"
                     /translation="MVKRNIIKCLVAVLSLTFVVAGCSKKEEVKDEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVAASVNKD
                     NLFEEKVYGDGTNGLQDLDLGRVSAWIDAELTVVGDAKKANMKVRVLDEKLSVTNIAY
                     FFKKNDEKSKTIKEDVNKAIEEMLSDGTVKKISEKWFGMDVSADIQK"
     gene            13556..14731
                     /locus_tag="MIW95_002717"
     CDS             13556..14731
                     /locus_tag="MIW95_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY3152029.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEFKTSKLIKEE
                     LNKLKIEYIDVAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKILENIV
                     RNVCRAYGADCKFEKSTGGDAVINDKNMIELGRESACKILGVENVEIIDFPAMTGEDF
                     AIYMKEKPGLFMYIGVGNKEKNINYRLHSNKFNIDEKCLSIASSLFSQLAVDYLCQL"
     gene            14840..15589
                     /locus_tag="MIW95_002718"
     CDS             14840..15589
                     /locus_tag="MIW95_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBY3152030.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEINQSEVVAILGPSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDIANLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSNKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIVRKEEVFS"
BASE COUNT         6094 a         1656 c         2582 g         5331 t
ORIGIN      
        1 atattattat tagtaataac tttaaaatta tattactatt atataaaaat tggcttaaaa
       61 gaaggtgaaa aaagtaaatg aaaatatgca taacagtagg tcatagtata ctaaaaggtg
      121 gtaaaagtac aggtgtaaat ggtatagttg atgaatacaa ttataataaa aaattagctc
      181 ctatgttagc tgagatgctt ataagtcaag gtaatacagt agatgtaata atttgtccag
      241 aaaggcactt tatgtcagaa agagaagaat ttttctatag agttccaaaa gtaaacagtg
      301 gtaaatacga tttattagta gagctacatt taaataaagc tgatggaaca caatgtggta
      361 cagaagtgct ttactatggc actgaaggat tagaatatgc taaaagagta tctaatagac
      421 ttggagaatt atttgaaaat agaggagcta aaaaaagaga aaatttatat atattgagaa
      481 atacaaatcc agttgcaata cagattgaaa gttttttctg tgataatgtg aatgattgta
      541 acaaagcaaa tgaatcaggt tatgattata tagcacgatt gattacagaa ggtatattga
      601 ataaagatat aaatatgtga gattatggtg atttaggtat tgattaatac cttttaatat
      661 tagatataaa taaaaataca atgtattagt ataatttaaa ctttcaaaat atagattagt
      721 ttgattattg acaagagata cctcctaaat attgatttat cagtatattt aaggaggtat
      781 ttaaatataa aaatttaatt gatttgctac taaactatag gtttatagag tatatatttt
      841 tatcttcagt ctaataaata atataaaaat agtagaacct tgtattataa taatataatg
      901 tagaaaaata tatagataag ataaatataa taaggtggta atatatgaaa tatacatttg
      961 aaaaatttaa gttaaatgaa aagatactta agtcattgaa gagtttagga tataatatac
     1021 cttctagagt acaaagagaa gttattccaa aattattaaa aggtcaaaac ttagtagtta
     1081 ggtcaaaaac aggaagtgga aagacagcaa gttttgcaat acctttatgc gagaatataa
     1141 atgtagattg caataatatt caagctctta tagtggttcc aacaagagaa ttagccttgc
     1201 aagtaaaaga tgagatatca gatataggga gactaaagaa ggtgagatgt agtgctatat
     1261 ttggcaaaca atctataaaa gaccaaatag ctgaattgaa acaaagggtt catattgttg
     1321 tagctactcc aggcagaata cttgaccata taaataaagg ttctattaag ttggaaaatg
     1381 taaagtattt agtaatagat gaggcagata aaatgtttaa taaaggtttt gttgaacaaa
     1441 tggaaaaaat attattaaat ttacctaagg agaaaatagt aagtcttttt tcagctacaa
     1501 tagatgaaga gataaaatat atatgtgaaa aatatatgct agattacagt gttataaata
     1561 tagaagaaaa tgagagcgat actaatcaaa aaacaagaca aatagatgac aaaataatca
     1621 aggcaaatgg aagagagaag tacatccttt taaaagaatt aatatacagc gaaaatccaa
     1681 agagtgtgat aatattttgt aataccaaag agaaagtatc taaattatat aataagatga
     1741 gtaaagaggg ttttttaata agagagttac atgcagattt atcacaagaa agaagaattt
     1801 ttgtaattaa ggattttaaa aaccaaaaat ttaatatatt agttagtagt gatgtagcgt
     1861 ccagaggaat acatattgat gatatttctt tagtaataaa ttatgatgtg ccacaagata
     1921 aagaaaacta tatacataga ataggtagaa ctgggagaaa aggaaatagt ggaaaggcaa
     1981 ttactatagt aactgaaaag gatgaaaaat atatagagaa tatagaaacg tatataggat
     2041 ataaagtaaa tgaattaaaa gatatagaaa aaagtagaat tacacatggc aaaattttgt
     2101 ttgaagaata ttcaaaagga atcctaaaaa aagtatcaaa gagaaaaaaa gttgataaaa
     2161 attcttacaa aagtaaagat ataaaactaa atatagaaag tgaagttgtt aaattatatt
     2221 taaatgctgg taagaagaag aaaataagag ttttagatat agttggtgca tttagtaata
     2281 taaaaggtat aactaatgat gatataggtg taattgaagt tcaagattta tgttcatatg
     2341 tagatatatt aaattataag ggagatttga tattaaaaaa atataaggag atacctataa
     2401 agaaaaaaat ggttaaagtg aaaagagata tacgataatt tagttattac tgctctctct
     2461 gatgatgaaa tcatatattt aagtaagagt atcaatattt atgaattatt ctgttatcga
     2521 aatgaagttt caaaaaacaa tagaagttgt taaggaaagg aatgttgact ttatctacat
     2581 tctaagctat actattttta aatgtttatg acaatagtca ttttaaatat aagtaataaa
     2641 tatataccta tagggatatt aaaggaggta atatatggca tattttaact ataacgacaa
     2701 acagtgctac tataatgaaa ttggggatgg tacaccatta ctttttttac atggaaatac
     2761 agcctcttcc aaaatgttta atgatataat cgatttttat aaagatgaat ataaagtaat
     2821 attaattgat tttgtaggtc atggaaaatc tcaaatggtc gataaatttt ctgctgactt
     2881 atggtttgat gaggctatgc aagtgatatg ttttttagaa gcaatgaact ataaaaaagt
     2941 aaacataatt ggtagtagtg gaggagctct tgttgcatta aatgttgcac ttgagagacc
     3001 tgatttagtg aataaggtaa ttgcagatag ttttgaagga gaagttcctt tagagtcatt
     3061 tgtacaaaat gttaaaatag agcgtgaggc atcaaagcaa gatgatggtg caaaagcttt
     3121 ttatatttat aatcaaggag aaaactggga aaaagttgta gataatgata cagaagctat
     3181 ttttgaacat tataaaacta taggaaaatt ttttcataag cctctagaaa ctatgcaacc
     3241 agaggtgctt ttaacaggaa gtagagaaga tgagtttgta tctctaatat ctagtgattt
     3301 ttttgaaaat actttttcat tacttcttga aaagattaaa aatggtaaaa tgtatttgtt
     3361 tgataaaggt ggtcatcctg caattttatc gaatggatta gatttttcaa atgtagcaaa
     3421 gaaattttta gaggaataat ttaaccaaaa tttacaataa tgaaaactgt aaaatggatt
     3481 gcaaaactta caaaagtgag tgtacatata cttcattatt atgatgaaat aggattgtta
     3541 aaacaaaata ttctaatagt caagttatta catttgtata tgcaatatgt atagaaagag
     3601 atatcattag attgtaaagt tgggattaaa taacaatatg aaattttatt tattcagata
     3661 ttgtagctta actatataaa tgttagaata aatttatata gttaagctgt aggattaaat
     3721 aatttagtta aataagtcta ttatctcttc atcagataga ctttttaata caccttcatt
     3781 agaaagttct ccatttataa attgatttat aagctctttt ttactttctt gtaattttat
     3841 tattttttct tctatagtcc ctttagcaat caattttata acttgtactg aatttttttg
     3901 accaaatcta tgagctctat cactagcttg attttctact gatggattcc accatgggtc
     3961 aaagtgaatt accatgtcag cagatgtaag atttaaacca gttccgcctg cttttaaaga
     4021 tattaaaaaa acttttttat ccatactatt attgaactca tctacaagtt caagtctttc
     4081 tttggcatta gttttaccat ctatataatg gtatttaatt ttatacttat caagttcctt
     4141 acttatgttc ttaagcacag aagtaaattg tgaaaacaat aatattttgt ggttttcgtt
     4201 tatactatcc ctaagtattt ctaaacatgt ttcaattttt gaactttttt tattgtaatc
     4261 ttttactact atacttgggt ctaaacaaag ttgtcttagt ttagttaaat aagaaaatat
     4321 aactatttta tctttcttta gatttttatc tttcatttta tcttgtatat cttttgaata
     4381 tacactatat atctttttct gctctttatt caattcaaca aaaaagtttt tttcaatctt
     4441 atctggaagt tctttcataa cttgtttttt acttcttcta agaataaaag gttttattaa
     4501 ttttttcagg tttttaacat tatcctcttt atttataaac aattcctgaa actttgcttt
     4561 actatataga tagccaggca ttataaaatc aaatatagac cataattcta gtagattatt
     4621 ttccataggt gtaccagtga gtgcaaattt attttctgca tttatacttt ttacagactc
     4681 agttactaaa gctactggat tttttatatt ttgagcttca tctaatatac aataatcaaa
     4741 ctttatttca ctatattttt ctaaatcatt tctaagagta ccatatgttg taagtattac
     4801 atcaaaattt tctaattcca taaaacattt ttctctatct cttttattcc catgtatcaa
     4861 taaaactttt atatctggag caaacttttc aaattcattt ttccaattat aaatgagtga
     4921 tgtcggtgta acaataagac tttttttatt agataatgat aataaaaatg caatagtctg
     4981 tattgtttta ccaagaccca tttcatcagc taatatacca ccaaatttat aataatctaa
     5041 aactttaaac caattgaggc catttatttg ataatcacga aggtttgcat ttaaatttgc
     5101 gggaatttct aaatttatgc tatcaaaatt tctaaatttt tcacatatac tgtctagttc
     5161 ttttttacca tctataaact ttaaattttt actacgtatt acatcattta tatatagagc
     5221 tttattttta taaattttat tattttttga tttatccatt atatctagat tttcaactaa
     5281 ttcaaaaaaa tcttttgttt ctctttctga taaatctata aagtttccat ctttaagttt
     5341 ataaaaagta cgtttttctt gaaaagcttt caaaatatcc ttgtactctt ttggatttat
     5401 atcagatata ttaaaattaa attctaggta attctggtta gtctgattga ttgaagcttg
     5461 aattgatgaa gcgttatata tttttttcaa tttaaacttc tcagaataat atatatctcc
     5521 aaattccttt agagattcta attgtaaatc taaaaatgta aataggtcat agtcagttcc
     5581 tttaaacaca taatgattat tttcctttgt aaattgtgaa gaagtaagtt tattttcaat
     5641 agtagtttcc ttttgtatgt tggggagcac aaatttttta tcctcatcat catatatata
     5701 ctttacatca caacatatgt tgccatcaat catatcaaag tagaactcag atttaaaatc
     5761 ttttgttata ttatttttta tgttatcatc taaatgaact tcttgagata tagattttaa
     5821 tctaggaact agcaatccta aaacatcctt agaatcctct ttatcaaatt ttaactcttt
     5881 agtctttgat aatatttgat atatttttga ataatcatga gcttttttat gtgataataa
     5941 atagatattg ccatcatata aaagtacatc atttttttta gtaagtggaa caggtagtga
     6001 atctttagaa tataagacta ttttatcttc tatattttta aaatcaaacc ttacaggtag
     6061 agtatcattt aatatctttg gatgatattc ctctttagaa aaaataaggg taaaatcctt
     6121 gtacttaata gcatttaaaa attgttttaa agagcttgca tttatctcca taaatttttt
     6181 tgaatgtgat gaagaaaagt tgtatgaaag atttgtccca tattcttcta taatatctac
     6241 taacttctca tcttcatcag caaaataatg aatgttgggg ttgtaagtaa atttcttccc
     6301 aaaatctaat tcatatccat taagttttga atatgcaaat tcagtaatgt tttttaaaat
     6361 gtacatttta tcaattccta ttttaaattc agcaattgct ttatttgaat ccacatttat
     6421 caatacatct aaattaaccc tttctctttt agtattgtct aacaaagaaa gtaattgctt
     6481 gcttaaatct aagcttgttt tagaatttgt attagaaagc ttatccttat ctgcacctaa
     6541 tatatctatt agaataggag gaacatctct tttatttttt atttgtttta agctttttat
     6601 cacaatagca actatatgct tacatataaa aatacttttg ccctttgatt gacagtcagc
     6661 gcaactacaa tcattttcaa ttatcttaaa gtgctttata tctatagtta ttgaattaaa
     6721 ataaaagcta taattatctt cagaattgac tccaccacga aatgtcaaca ggttattttg
     6781 taggtcatca atatctatgt ctatataatc aactaattga ttagtataat attttacacc
     6841 tcttgaccat cttgatggtt ctgtatttct tcttattagt ttatatatca tatctatttt
     6901 catattattc accttacctc actgtatata ttataatcta aatgcattca atttacactc
     6961 taaatttaag actaaaatta aggcagatat gaatagcttt tatctattac aatatatgtg
     7021 taaataacaa tttactatta gatttttatt gaattatctt ataaactatg aatgaaaaat
     7081 aattatataa atagattatt aataaatata aaatcttatt attaaaaata ttcttagaaa
     7141 ttgcactata taaaatataa agtatgatat ttgaataaaa agaatgtata ataagtataa
     7201 taatttgtat ttattattgg gaaaggaaaa tgttaatatg gatagttata gacaacttaa
     7261 agctaaaata ccctgtaagg aaacaggtat agaaataaag aggactttat gtgatatttg
     7321 ttctccagga gtacattgtg gaattgatgc atatgttaaa gatggtaaag taataaaaat
     7381 agaaggtaca aaagaacatc caatgaataa agggttgtta tgtaccaaag gtctttgcaa
     7441 tagagaatat atatatagag aagatagaat aaaaacacct ctaaaaagaa ttggaaaaag
     7501 aggtgaagga aaatttaaac caatatcatg ggatgaagct tatagagaaa tagcagaaaa
     7561 attaaataga gtaaaatctg agtatggagc aaagcatgta gcttttttta cgggttatgg
     7621 aaagtggtat agaccttttt tgcaaaggtt tgcatcttca tttggttcac ctaattatgg
     7681 tacagaatct agttcatgtt caacatcagc acagatggct ggaaatgttg ctactggaag
     7741 aaactttaca atgcaagata tggagaatgc aaaaacttat ttagggtggg gatttaatcc
     7801 atactactct aaatttttaa atagtgtgaa tctaataaaa ttaaaagaaa aaggtctaaa
     7861 atttataatt gtagacccta gaataacacc gacaactcaa aaactagcag atttacattt
     7921 aagaattaaa cctggaactg atggagcttt agctcttgga atggcgaatc tatttataca
     7981 aaaggattta atagatagag aatacataga aaagcatgta catggattta atgaatataa
     8041 agaatatgta aaattatttg atttagatac tgtaagtaga attacaggag tttctaaaga
     8101 taatatagta aaagctgttg agatgttgtc acaaggtcca ttttgtataa atgaaagtgc
     8161 agctccaata gttcatcatc aaaatggatt tcaaaactat agagcaatga tgtctttatc
     8221 tgctataaca ggtaattatg atagaatagg tggaaacata ccaaaacaag attactttgc
     8281 tggtaagata ggtggattcc aaacactaga aaaagagttt gtaaaagaag ttctacctaa
     8341 aaatataaat gaaagaattg gtggagaaag atttcctcta tggaatgaaa tggtaactca
     8401 atttcaagct atggatttac ctaggcaaat agaagaaaag acaccatatg aaataagagc
     8461 tttgttttgt catgggataa atgctagaat gtttcctaat tcagggagac tttttaaagc
     8521 gttgcatagt gtagattttt ttgtgaatat agatttattt atgacagata catcaaaata
     8581 tgcagatata cttttacctg cgtgtacatc atttgagaga gaagaatgta agagttaccc
     8641 aggagggtat attacatata ccaaaaaagt tattgataaa ttatacgatt caaaatcaga
     8701 tgtagaaata ctttcagatt tagcaaatgt tatgaatata gatgatgatt tattaaaagc
     8761 aggttatgaa gcaagtataa gacatatgtt taaaaagacg tgtgtagatg tagataaact
     8821 taaagaaagt gatttaccat taaaatgtaa agattttaag ccatatatag taggctcata
     8881 tacaagagag gggtatgata ctagcacttc taggtttgaa ttaaaatcta caattattga
     8941 aaaatataaa gattttggac tggatgcact accaacatat agaggattta atgataatat
     9001 agataatgaa tatactcatt tattgacatc aggagcaaga atttcaaatg cactacattc
     9061 aagacttcat gacatcaaac tttcaagaat attaaaaaat gaagctagtt gtgatatttc
     9121 cagcttggat gcaaaagaat tgggtgttat cagtggagat tatataaaaa taactactaa
     9181 gataggaagt ttggatgtaa aagtcaatgt tactgataga gtcttatata agaatataca
     9241 tatgtatcat ggatatagag aatctgatat aaatagtatt attgatgata attttgaccc
     9301 ttatagtgga ttccctgcgt atagaagttg caagtgtaag attgaaaaaa tgaatagata
     9361 aatggagaga tataatgaaa agtttattta aatttaataa agaattatgt acagcatgtg
     9421 gagcatgttt gcttgcatgt atagaccaaa atgataataa tatagacaat ggtgaacttt
     9481 ttagacatgt atttacaata gaggaaggtg aaaatataga atatttttca ttagcatgta
     9541 tccattgtaa tgagccaaaa tgtttaggtg catgtcctaa aaattgtttt aaaaaagaag
     9601 aaggattcgt tgtattagac aatcaaaact gtatagggtg taaactttgt gaaaaagctt
     9661 gtgaatatgg agccttaatt tatggaaatg atggtaaggc aaataagtgt aatggttgta
     9721 ttgagagata taagtgtggt atggattcac cttgtgtaat ggtatgtcct acaggagcat
     9781 taaaatttaa taaataaatt aaatatagag agtgacttat acttggtata agatattttt
     9841 acaaaaagct atattgagtt tgttataata aaaactcaat atagcttttt tatggatata
     9901 gttggatact ataatgtgat ttttagtaat agttttttgc tattggacaa agactgtaaa
     9961 acatattaaa cttatgttgt tcaaagtcga agtataatat aataactaga aaattgattt
    10021 atagtgttat aaaagcaaat ttagtaaggt ataaaataag aggcaatctt ttactacagt
    10081 atcaatattt gggagttatg ttatggcaga gccattaaac aattataatg gtacaagtga
    10141 ttcaaccttt gaaaatgtat tatataaaaa attaaaataa gattaaaaag gagagaataa
    10201 attatgaaaa aacttaaatt aattattatg cttgccttgg tattagcact tttaattaca
    10261 ggatgttcat caggaaataa taaaaataaa gaagaacaaa aaactataag agttgcaaca
    10321 tcaggaacat attacccttt tgcttttaaa gataaagata aacttaaagg atttgaagta
    10381 gatttttggg atgaatttgc taaacgaact aattgtaaag ttgaatgggt attaactgat
    10441 tttagtggat tatttggatt attagaagct gataaagcag atgttgtatc tgctcaactt
    10501 actgcaacac ctgaaagaga gaaaaaatat gctttttcag atgcttataa ttatagtgga
    10561 acaaacatta ttgttagaga agatgatact agtataaaat caatagatga tttagttggc
    10621 aagaaagtag gtgtgggtac tggagcagta gcaaatgaaa tactaaaaga aaagtaccca
    10681 aatgatgaaa taaaaatagt aaattattca agtgcaactc ttaaagggaa ttatcaagat
    10741 ctagaaatgg gaagattaga tgcagtagtt gcgcaggatg ttgaggcttt aatagctata
    10801 aaagaacaaa aattaaatct taaaatggta gaacctccaa ttcaattcgg agcctgttct
    10861 tttgcgattc aaaaaaatga aaaaggtgaa aaatggacaa aagaaattaa cgaagtaatt
    10921 gaagagatgc ataaagatgg gactttgact aaaatatctg aagcctggct aggaaaagac
    10981 attactaagg agcctattaa tgagtaattt taattttgaa ttttgtctaa gtatatttcc
    11041 atttttgatt aaagcattgg gaataacaat actaatatct attgttgcat tttttttatc
    11101 aatattatta ggtgtaatac ttgccttttt cacacagaca aaatcaaaag ttttgcaata
    11161 cataacaaga gtgtatgttt cattctttag aggaacacca cttttaattc aaatattttt
    11221 cttttacttt ggatttccaa tgatattttc ttttatgaaa gattgttcac cttatttagc
    11281 aatagtaatt tgtttaggtg taaattcatc tgcatatatg actgaaatta tacgagcatc
    11341 tattgagagt gttgataaag gacaacagga agcatgttat gcttttgggt taagtaaata
    11401 tcagtcaatg gttaaaataa ttctacctca agcagctaca tctgcagtac ctcctattgt
    11461 aagctgtaca ctggacataa ttaaaggttc ttctttaggt ctaactatag gaatacaaga
    11521 tttaatggga gtagctcaag tagaggctgc ttcaagtttt aaatattttg agatatatat
    11581 gataacgata ttaatttact gggcagtatc tataatattg agtaatatac aaagcaaagt
    11641 tgaaaaaaga ataggaaagg catattagat ttcaatattt aaaaatggga tgatgtagat
    11701 atattattac ttgtaaataa atttttaatt ttagaaaaat aaacttagta atctaaatta
    11761 ttgagaaaat atagtatcaa aatggactgt ataatcaagt taaaaaatag tttttctatt
    11821 gaaaaaataa ataaaattct attgtaattt aatattcgac aaagttttat aaaaaacaac
    11881 attaagctag ggggacttat tttatgattt ttaatatgaa atatttttta gaattatttc
    11941 caataataat gaaatcgttc aatgtaactg taattttggc aataagttca ttagtatttt
    12001 cattaattat aggtacaata gtagcattat catcttatta caaagtgaaa gtattgaatc
    12061 aaatatgtaa agtatatgta tctatcttta gaggtacgcc tcttatgcca cagttattct
    12121 ttttatattt tggactagcg tatatgaacg attttgttaa aaatatggac ccagtatttg
    12181 caacatctat agtacttagt ttaaatatgg gagcttatat gtccgaaacg ataagggcat
    12241 ctatactttc aattgataaa ggtcagatag aagcagcata tgtaatggga ttaacaaatt
    12301 tacaaacaat gaaaaggata gtgataccac aggccgtaag agttgcactg ccatcattat
    12361 tcaataattt tatagattta attaaagggt cttctgtagc ttttgttgtt ggagtaaatg
    12421 atataatggg tgctgccaaa tcacaaggag cgttgagttt taagttcttt gaagtgtatg
    12481 gagcagttat ggtaatttac tggtgcataa taactccttt agggtggggg caaaaaaaat
    12541 tagaaaagaa aataagcaat tattaagtta ttaagaaaat acataaaagt atttttatat
    12601 aaaaaatggt gtgaggagaa gagatatggt aaaaagaaat attataaaat gtttagtggc
    12661 agttttatcg ttaacatttg tagttgctgg ttgttcaaaa aaagaggaag taaaggatga
    12721 aaaagttata actgtagctt catctaattc agctgaccca tattcttatg ttgataatgg
    12781 tgagcataag ggttttgaag ttgatatgtg gaaagagatt ggtaaaagaa gtggatataa
    12841 gataaaaatg gaaccaacag gttttagttc tatatttggg atgttagatt ctggtaaagt
    12901 tgatgtggca gccaatttct ttggaatgtc taaagaaaga ttagaaaaat acgatgcttc
    12961 tataccttat ggttctgatt ctgtatgtat agctgtaaaa gatggaaaca acgaaattaa
    13021 taaacttgaa gatttaaaag gaaaagttgt agcagtttca gaaggttctc aagggcaaca
    13081 ggttgcagct agtgttaata aagataattt gtttgaagaa aaggtttatg gtgatggaac
    13141 taatggtctt caagatttag atttaggaag agttagcgca tggattgatg cagaacttac
    13201 tgttgtagga gacgctaaaa aagctaatat gaaggtaaga gtattagatg aaaaattatc
    13261 agttactaat atagcatact tctttaagaa aaatgatgaa aaatctaaaa ctataaaaga
    13321 agatgtaaat aaagcaatag aagaaatgct ttcagatgga acagtgaaaa aaatatctga
    13381 aaaatggttt ggaatggatg taagtgctga tatacaaaaa taaatacttt aaaattaaaa
    13441 tataaaactg tgataactat agataagtat actaaattta tctatagtta tctgtgtatt
    13501 acaatgtatt tagattagtc tttataaatt aaaatgagtt atggaggtct tgcaaatgat
    13561 tgaaactaaa ataaaatcca ttgcactaaa aaatagagaa aaaatcataa aaataagacg
    13621 acagatacac agtaatccag agttagcatt taaagaattt aaaacatcca aacttataaa
    13681 agaagagtta aataaactta aaatagaata catagatgtt gctggtacag gagtattagc
    13741 tactataaaa ggaaaaaata atggtgggaa aactatattg cttagagcgg atatggatgc
    13801 ccttccaatc aaagaggaaa atgatttaga atttaaatca ataaatgata atatgcatgc
    13861 ttgtgggcat gatgcccatg tttcatggtt gttaggaaca gcaatgattt taaatgacat
    13921 aaaagaagaa ttgaatggaa atgtaaaatt actatttcaa cctggcgaag aaaaaggtgg
    13981 ttctgatatt gtaattaaag aaaatgtact tgaaggtgtg gatgcattag caactggtca
    14041 ttgttggcca actattgaat caggaaaaat tggaatcgct agaaattgtg ctatggcagc
    14101 gactaatact tttgaaataa caataattgg caagggcggg catggagcag agcctcataa
    14161 ttgtatagac ccaattgcag ttggaaatgc agtatattca tccattcaac agatagtttc
    14221 aagaaaaatt gacccagttg tacctgtagt agtgtctgtt tgttctttta attctggtgt
    14281 ttctaaaaat ataataccag atgtatgcac acttcaaggg actattagag cgataagcaa
    14341 ggaacaagta attgaagtaa gtaaaattct agaaaatata gtaagaaatg tatgtagagc
    14401 atacggagca gattgcaaat ttgaaaagag tacaggagga gatgctgtta taaatgacaa
    14461 aaacatgata gagctaggaa gagaaagtgc ttgcaaaata cttggagttg aaaatgttga
    14521 aataattgat tttcctgcaa tgacaggtga ggattttgct atttatatga aagaaaaacc
    14581 tggtttattt atgtatatag gtgtaggaaa taaagaaaaa aatataaact acagattgca
    14641 tagtaacaag tttaatatag atgaaaaatg tcttagtata gcatcttcat tattctcaca
    14701 acttgcagta gattatttat gtcaactata gagtacattc ataaaaatat atttagccta
    14761 tttttaaatt taatgctagt atattaattt tgatgtagaa catattattt attatattta
    14821 tttaaaaagg tggataacaa tgataaaagt agaaaattta cataaaagtt ttaaagggac
    14881 agaagttctt aaaggtataa atttagagat aaatcaaagt gaagtagtag ctatacttgg
    14941 accatctgga acaggtaaat ctacgttact tagatgttta aattatttag ttgagcctac
    15001 aaaaggaaag ataatgattg gtgatgtaaa agtagatgta gaaaaagcaa caaaaaaaga
    15061 tatagccaat ttaagaaaac atacatctat ggtatttcaa aattataact tatttaaaaa
    15121 taaaacagca ttagaaaatg taatggaggc attgatagta gtacataaaa agagtaataa
    15181 agaagctaga gaaatatctt taaaattaat agataaagta ggtatgttgg ataggaaaga
    15241 tttttatcca tcaaaattgt ctggggggca gcaacaaagg ataggtatag caagagcgat
    15301 ggcagtaaat ccaaatgtaa tattatttga tgagccaaca tctgcacttg acccagaact
    15361 tgttggagaa gttttaagcg ttataaaatc gcttgcgaaa gaaggaataa caatgcttat
    15421 tgtaactcac gaaataaatt ttgcaaaaga agttgctgat aaaattatat ttatggataa
    15481 tggtgtaata gctgaagaag gaactcctga tgaaatattt aataatccta aaaatgaaag
    15541 aactgcaaaa tttttaaata tcgttaggaa agaagaagta tttagttaaa agtgtataaa
    15601 ttaagtatta gaagatatgt aagatactcc cattaaattt ataagttaga taataaattt
    15661 aat
//
LOCUS       DAGQWR010000101        15410 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.101, whole genome shotgun sequence.
ACCESSION   DAGQWR010000101 DAGQWR010000000
VERSION     DAGQWR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.101"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            64..771
                     /locus_tag="MIW95_002719"
     CDS             64..771
                     /locus_tag="MIW95_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152031.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            818..2413
                     /locus_tag="MIW95_002720"
     CDS             818..2413
                     /locus_tag="MIW95_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152032.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADSREKQKIMGMILSVVIITG
                     LLIFYVTSISLSLVDILKQINQMEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFFNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFMFIGQISSRIKYKNLILIIGSIILMLLYTLLMTRLNSMSVEMLKN
                     SVSISETIRKIYPPIYYFVDALKTGSVVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKTYKFKSQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIIGTILILL
                     FGADKVGELLKLNIDFNFIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLGFEIQFIIVSICLIASFSMFVALMGLLS
                     NLLYPNLDWKNEMAVVKRSASMIITMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TFILDFMIWRIISTKGVRIFKNL"
     gene            2516..3598
                     /locus_tag="MIW95_002721"
     CDS             2516..3598
                     /locus_tag="MIW95_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBY3152033.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            3747..3908
                     /locus_tag="MIW95_002722"
     CDS             3747..3908
                     /locus_tag="MIW95_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBY3152034.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            4249..5517
                     /locus_tag="MIW95_002723"
     CDS             4249..5517
                     /locus_tag="MIW95_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBY3152035.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            5712..6500
                     /locus_tag="MIW95_002724"
     CDS             5712..6500
                     /locus_tag="MIW95_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBY3152036.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            6500..6949
                     /locus_tag="MIW95_002725"
     CDS             6500..6949
                     /locus_tag="MIW95_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBY3152037.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            6953..9217
                     /locus_tag="MIW95_002726"
     CDS             6953..9217
                     /locus_tag="MIW95_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY3152038.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKNALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            9357..9668
                     /locus_tag="MIW95_002727"
     CDS             9357..9668
                     /locus_tag="MIW95_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152039.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            9954..10454
                     /locus_tag="MIW95_002728"
     CDS             9954..10454
                     /locus_tag="MIW95_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBY3152040.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIISGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            10511..11497
                     /locus_tag="MIW95_002729"
     CDS             10511..11497
                     /locus_tag="MIW95_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBY3152041.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            11547..12269
                     /locus_tag="MIW95_002730"
     CDS             11547..12269
                     /locus_tag="MIW95_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBY3152042.1"
                     /translation="MENFNKNAKIIGIGVEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            12298..14232
                     /locus_tag="MIW95_002731"
     CDS             12298..14232
                     /locus_tag="MIW95_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY3152043.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFIEESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALDGGYFFPVRWIEYGKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            14225..>15410
                     /locus_tag="MIW95_002732"
     CDS             14225..>15410
                     /locus_tag="MIW95_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBY3152044.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKIRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDECSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLGYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKF"
BASE COUNT         6115 a         1427 c         2663 g         5205 t
ORIGIN      
        1 ttgtttgata tttgttatat attatatata acaaatatca aaactataat atggagggaa
       61 attatgttaa aagtaatcaa tgtatctaaa tcatataaaa ataaaaaagt agtaagtagt
      121 gtctcatttg atgtaaatga tggagagctt tttgggttta taggacataa tggagcaggt
      181 aaaactacta ctattaaagc aattgttgga atacacgatt ttgaagaggg agaagtacta
      241 ataaattcta aatctataaa aaaacagcct gttgaatgta aaaaggaaat ggcatatata
      301 cctgataatc cagatttata cgagtcatta acgggtatac aataccttaa ttttatagct
      361 gacatatttg aagttgccaa agacaaaaga gaagagttaa taaaatatta ttcaaataag
      421 tttgaactca ataaagcatt aggagattta atatcttcat attcgcatgg tatgaaacaa
      481 aaattagcta taatttcggc tttgatacac agcccaaaaa tcttaattct tgatgaacct
      541 tttgtaggtc ttgacccaaa agcttccttt atattgaagg aaattatgag ggagttttgt
      601 gacaaaggtg gttgtatatt tttctcaaca cacgttttag aagttgctga aaaaatttgt
      661 gataagattg ccataataaa agatggaaat ataattgctt atggaactac agaagaagtt
      721 aagggaagta attcacttga aagtatcttt atggaattaa tagagaaata aaatgttttt
      781 tatacaatat agtgtctaaa gtgaggaatg ataaaatatg agcaatctaa gtatattaat
      841 aaaaactaat ataataaatg aatttaaatt aaatgcgtta aaaaaagctg atagtagaga
      901 aaaacaaaaa ataatgggga tgatactttc agttgttatt ataacagggt tattgatttt
      961 ttatgtaact agtataagtc tttctttagt tgatatttta aagcaaataa atcagatgga
     1021 aatgctactt atatttggat ttggattatc aacattaaca actattgtta catcattata
     1081 taaaacgtca tcatatctgt ttcaagcaaa agatttagac cttttgacta gtttacctat
     1141 aaaagaatct actgtactag caagtaagat attaatgttg gttggagcaa actatttatt
     1201 ttcttcaata tgtatgatta taccagcagt ggtttatttt tttaattctg atgtaagtat
     1261 aacgtatttc ccttatttga tactgctatt tttagcttta ccattattac caatagttgc
     1321 ttcatcaata atttttatgt ttataggaca gatatcatca agaataaaat acaaaaattt
     1381 aatattaatt ataggtagta ttatattgat gttgctttat actctactta tgacaagact
     1441 taactctatg tcggttgaga tgttaaagaa tagtgtatct atttcagaga cgattagaaa
     1501 aatatatcca cctatatatt attttgttga cgctcttaaa acaggaagtg tagtttcctt
     1561 tttagcattt atagcaattt caataatacc attttttatg tttgtaacat tatttgcaaa
     1621 aaaatttaaa gatataaatg ctaagatgag tgaaggatat aagtcaaaaa cttataaatt
     1681 taagagccaa aaaacttcaa gaccttcaaa agctctatta aaaaaagaat ttaagagata
     1741 tacttcttca tttatatact ttttaaattc atctgcaggt ttgttattcc taataatagg
     1801 tacaatttta atattattat ttggagcaga taaagtgggt gagttactga aattgaatat
     1861 agattttaat tttataaaaa tacaattatt gggagtattc ttctttataa tagtaatgaa
     1921 ttgtacaaca tactgttcaa tttctcttga aggaaaaaat ttatggatac tgaaatcttc
     1981 tccaatagat gaaagagaaa tattctggtc aaaaatattg atgaattttt tattgaatgc
     2041 accattatct attttatgtt taatattagt atctataaga ttaggttttg aaattcagtt
     2101 tattattgta tcaatctgtt tgattgcaag tttttcaatg tttgtggcat tgatgggatt
     2161 attatcaaat ttattatatc caaaccttga ttggaaaaat gagatggctg tagtaaaaag
     2221 aagtgcaagt atgataatta caatgttagt atcattagct tacgtaatta tacttggagg
     2281 tatatatgta ttacttggaa tttcgttttt aaatatatat attattttgg caagtatact
     2341 tacttttata ttagatttta tgatatggag aattatttct actaaaggag taagaatatt
     2401 taaaaattta taggtgtatt tatgtcaaat aaagacttat cttttttagt cttgcaaata
     2461 ataggttaag tagtatacta tgtatagtga tatgaaaaaa tttaggagaa aataaatgag
     2521 aaaattatcc aaaagagaaa aagattttat tcaatatgga tttttattat ttttgatatg
     2581 tattactact tatttagtat taactacttt agatataaaa ctaataccta aaattataag
     2641 gctagtaaat aagacttata tatttcttgg catattgaca attatagtat atatgataat
     2701 tgaatctatt gtaactcatt tgataataaa tgcagttcaa aaaacaaaag taaaatttat
     2761 tggtattaaa atggctacaa tgggatttta ctataactta gttactccat ttgcatctgg
     2821 gagtcaacca atgcaaatat atgcacttac aaaatatgat gtaagcctta gtaaatcagt
     2881 ggctatagtt actaataaaa ctgtagtatt tcaaagtaca gttactgtat tttgtggaat
     2941 tttaattttg ctaaataagt cattactgat ggagcaagta cattctgtga aagtcttgat
     3001 aacttttgga atgattatga atgtaagtat gttgttaggt ggactattaa tagtatttag
     3061 tccaaaacaa gtaaaaatga tagcagaagt tttgattaat tttttatcta aatttaaaac
     3121 atttaaattt ttagattcaa aacgtgatag aataaatcat tatattgatg attataatta
     3181 ttctataaaa atatttatta aaaatgtaag aatcttagtt ttaagtatat tacttactgt
     3241 gttacaatta atcgcatatt tcagtatagt ttattgtgtg tataaagcct caaatttaag
     3301 aggagtttca tatgtacata taatgacatt acaggtcttt ttatatatgg ctatatcacc
     3361 aattccaaca ccaggaaatg ttggagctaa tgaggttaca ttttttacta tgttctccaa
     3421 tataattcca aaagaattat tggggtactc tgtactctta tatagtgggt ttgtatacta
     3481 ttttatatta atagtttcag gtttatttac atttggaacg cattatacat taaattcttg
     3541 gaaaaaggaa gataaagtaa attctaattt agataaggtt attgaaaaat taaattaata
     3601 aaataattag ggaaattcta tatataaata tggtgttgag taagctatat tatattgaga
     3661 tatttccctt ttttgattaa aattatagaa atttgaaata ataagtaata aacagaaaaa
     3721 gtgatattgt tttgaaagga gaaactatga aacaagataa acaaaatgaa aaacaaagtg
     3781 agatttctaa gataaaacaa agcaatgata ataaacttga aatagctcaa gaattacatg
     3841 taagtaaaaa tatagggaat atgcttggat atagaataga taagaaaaaa atgaatagac
     3901 caagttaatg aaaatgttga aaatggtaca ttttaaaaaa tatatataat gaaatgtttt
     3961 attgttaaat ttaaacttat tttaatggag gaaaaagtcg aaaaatatct aaaaagatat
     4021 ggatataaaa aatattaata tatatatact ataaacttga caaaatcaac cttttaataa
     4081 ctagcaaaaa atagcaaaat tatataagcg ttatttaatt atcaaaaatg aaagctaaat
     4141 tgcatgtcct aaaaaaaatg attgataaca tttatttgtg tgctataatt atatagtaaa
     4201 acaataagta tattgcataa tgtatgcaaa tcgggaggag agacggacat ggaagcatct
     4261 atacacacaa ttgccagcaa taatttattg ataatttttg ctattgtaag tataactgga
     4321 ataatttgta gtaagcttag tgaaataata aaggttccag atgtagtgtt atatttactt
     4381 gtaggtattt taataggacc atcattttta aaatttatag atattcgtgg atttcaaata
     4441 gaaaatcaac taatacttac atttggttct gcatttattt tatatttagg tggaaaagaa
     4501 ataagtttaa aagtattgag aaatgtgaag atatctgtat tcttattagc tactttagga
     4561 gttgtgatat ctgcttttat aatgcaacaa gtaataggat ttacttttgg gataagtgca
     4621 atgactgctt tactagcagg aactataata gcatcaacag acccagccac tcttgtacct
     4681 atatttaatc aggtaaagat aaaggatagg gtaaaacaaa ctgtaataag tgaatcagct
     4741 tttaatgatg caacaggagc tatactaaca tcagcagtgt tggctgtaat tttgtcaaat
     4801 aaattttcat taggtgaaaa tatttatgaa ctggggacaa tggttattgt cggagtttta
     4861 gttggtttaa ttacaggaat actgcttttg aaattagtta atgataaacc ttatggaata
     4921 tttaaagatt ttgcaccaat aatatctgtt atttcagttg tagttgcata tgaattatct
     4981 acaaaattag gtggaagtgg atatatgtct tgctttattg taggtataat aactggtaac
     5041 aagaaaaact ttaaaatttg gttaagtcaa aaatcttatg atgcagattt ttatgttgct
     5101 gaaactttag gtactatttg tcgtatggca atatttataa tattgggtag ccaagttaat
     5161 ttggctcagt taagtaaata ttttatgcca tctgtaatta cagtattagt attaatgttt
     5221 atagcaagac cactttgcgt tttagtttgt acacttgtag acagggaagc aaaatggagt
     5281 aaaagtgaaa tattatttat gatgtgggta agggaaacag gcgtaatacc agcagcactt
     5341 tgtggtatta tttcagcaat gaaagttcct ggatacgaag tgatttcatc tgtagtattt
     5401 atgactatat taattacatt agtaatacaa ggaagtacta caaaattagt agctaaaaaa
     5461 cttggattat tagaagaaga agtgataaat ataagtgaaa aagtatcaac tttttaaagt
     5521 tttgctttaa aatagtacct gttaatggct attttaatat tgtctaatga tgattattga
     5581 aatagtcatt tttttatgta taagtaattt acagaattta catattagac atatttatta
     5641 tcaaaatttg ttgaagctat gatataatta gttgtattta aaataagaat agcaaataag
     5701 ggaggaaatt tatgtttacg gttatggata ttgtacagcc agatacagta gaagaagcgt
     5761 actcaatttt aaataaaaga aaaactaacc aagtaattgg tggaagtgct tttttaagaa
     5821 tggggaaaaa aagaatagga acaggaatag aattatctaa attaaattta gattatataa
     5881 aagagtatga agattacgtt gagattggtt ctatgactac ttttagaaca ttagaaacaa
     5941 gttccataat aaaaaataat tttggaagaa taatagaaga ttcagttaaa aatataatag
     6001 gagttcagtt tagaaatgta gtaacagttg gtgcaacagt attttctaaa tatggttttt
     6061 ctgacttgat agtagcatta ctttcattag atacggaagt agaattatat aatattggga
     6121 gaattagtct tgaagagttt ctaaatagag attatgagaa agatttactt attaagatat
     6181 acataaagaa gacaaataag aatgcttctt ataaatcact tagaaatgca aagagtgatt
     6241 atccaattct aaatgtatct gtatcaaaac atatggaaca atttaagtta tgtgtaggag
     6301 caagaccgca gaaagctacg attgcaaaac aagctagtga atttctatct aacaatgaaa
     6361 tcaatgaaat taatatagat aaagcagtag aaatagcatc agaagaacta acttttggtt
     6421 ctaacatgag agcatctagg gagtatagaa aagctatgag taaagtactt ttaaaaagag
     6481 ctataatgga ggttatttaa tgttagtaaa gattaatata aatgggaaga aaaaaagtgt
     6541 tgatatagaa ccagaagaat atctggttga tactttaaga aaagttggta atttaagtgt
     6601 gaaaagaggt tgtgacacag gctgttgtgg tttatgtaca gttttaattg acaagaaacc
     6661 tacattatcc tgtgcaacac tgacattgag agctttagat aaagaaataa ctacaattga
     6721 aggtttagaa aatgaagtta aagaatttag tgaagttttg gtaaaagaag gagctgagca
     6781 atgtggtttc tgttctcctg gctttatact tactgtactt gcaatgaagg atgagcttga
     6841 aaatcctaca gatgatgaaa taaagcatta tcttacagga aatctatgta gatgtacagg
     6901 gtatatggga cagttaagag ctatcaaaac ttatctggag gtaaaataga acatgaatat
     6961 agttggaaaa ggaatcaaga aaatagatgg aatgagtatc acaacaggaa agcctgtata
     7021 tacagatgat ttatcagcaa aaaatgcttt ggtggtaaag atacttagaa gtcctcacgc
     7081 ttatgcaaaa ataaaagata tagatacatc aaaggcagaa atggttgatg gtgttgaatg
     7141 tgttttaaca tataaagatg taccaaaatc tcgatttaca cttgctggtc aaacatatcc
     7201 agagccatct ccatatgata gattaattct tgaagatgtt gtaagatatg taggtgacga
     7261 agtagctata gtggcagcta tagatgaaaa aactgcaatt aaagcaatga atcttatcaa
     7321 agtaaagtat gaagtactta cacctgtttt ggattttgaa gaggcaatag gaaataaaac
     7381 tatggtacat gatgaagata gacattgtaa ctttgatata ggtatgcaaa gagaaaaaaa
     7441 tatagtatcc catcatgatt atacaaaagg tgatgtagaa aaagtattaa gtaattgtga
     7501 tgtagtgatt gaagaaactt attatactca agctcaagca catgcaatga tggagacgta
     7561 tagggcatac aattatttag accatatggg aagattggtt gtagtaagtt caactcaaat
     7621 accatttcat gtaaaaagac atttatctcg tgcactagaa ataccagcta gcaaaattag
     7681 ggttataaaa cctagaattg gtggcggatt tggtggtaaa caaactgcgt gtgttgagat
     7741 tttttctgca attgttactt taaaaacagg gaaaccagct aaaataattt atgatagaaa
     7801 agaaactttt aattgttcaa caagtagaca tgcaatgaga ttaaaagtta gactaggtgc
     7861 tacaaatgat ggcataataa aggcaataga tataaaagca ctttctgata caggagcata
     7921 tggagagcat gcttctacaa ctattggttt ggttggagaa aaaactttac caatgtataa
     7981 taaagttgaa gcaacaagat ttattgcaga tgtggtatat actaataaga tgccagcagg
     8041 agcacttaga gggtatggag catcacaagg atgttttgct gttgaatcaa ctataaatat
     8101 gcttgctaat aaattaaaaa tggacccaac agatttaagg ttaaaaaatg tggtaacaga
     8161 aggtgaaact acatttgcat atggtaaaac tttaaatagt gtcgatttaa taaaatgtat
     8221 aaataaaggc aaagaactta ttggatggaa tgaaaaatat ccttgtaaac atcttgataa
     8281 tggaaaaatt agaagtgtgg gtatggcaat tactatgcaa ggttctggta tacaaggtct
     8341 tgataagtct tcagctgaaa taaaattgaa tgactgtgga gattatactc ttatgatagg
     8401 ttctactgat atgggtacag gaagtgatac gattttagct caaatggctg ctgaggttct
     8461 tgatacaagc attgaaaata taacagttat atcagctgat actgacttag ttccatatga
     8521 cccaggttca tatgcttcaa gtactactta tgtaacaggt atggcagtag ttaaggcagc
     8581 tcaagactta agagaaaaaa taatttctgc tggagcaact aaactaggtg ttgagaagga
     8641 tgaagtagaa tttgatggta taaaattaac tagtaaaaaa ggtagcactt tattattcga
     8701 tatagcagtt gattctacaa ttggtccaga tactaatcaa ttaataggtc ttgcttctca
     8761 tggtagccca gtatctcctc caccttttat agcaggtttt gcagaaactg aaattgacac
     8821 agaaactgga aaagttgatt tagtaaatta tgtagctgta gtagattgtg gaacggttat
     8881 aaataaaaat ctagctaaga tacaagttga aggtggaatt gttcaaggga ttggaatggc
     8941 attatttgaa gaagttagat acacagaaaa aggaagcatg gacacaaata catttatgca
     9001 atacaagatt ccagctagat gtgatattgg aaatgttaaa gtagattttg ttgaaactta
     9061 tgaaccaact ggaccatttg gtgctaaatc tgttggagaa gttgttataa atacaccttt
     9121 accagcaata caagaagctg tgtttaatgg aattggtgta agaataaata cactccctat
     9181 tacacctgaa aaagttttta tggaaatgaa taaataatta tttatgaaaa tttagactag
     9241 ttttacatgt taaacaacca gttatgtcat acctgttgaa agatatttaa taagtagtta
     9301 ttataatatt atgaatttta cttataaaat acaaaattaa ataaggggat gatgttttgg
     9361 ctggtaatct aaataatatg agagcagtaa ataattttag aggagataag aacattttag
     9421 aatgtttagt cagctttgag ggtcgttcaa taagtcagag aaaagtaagg gtatttttta
     9481 aagaaaaaca aaatcaaata gaaattgatt ttgcagaaga ggaaatttct aaattggttg
     9541 aaaatgttgt tttaaataca tcatatcaag aaatgttata tgatgaaata gagaaacaac
     9601 tggaaattga ttgtataggt acttggatga tattatctaa attaaaagat ggtagtagag
     9661 ttcactgatt ttaaaataaa ttattagagc ctaattttaa ggctcttttt tgctgttttt
     9721 aattaacaat agaaaattta tctttagttt gtatataatt ttaatatcat atattctata
     9781 tatataaaat tgaatttgta tgtattatat tgtataatat aaaatgaatt tggaaaatag
     9841 ttcgctaaaa taatttgttt ttataaaatt actgtttaag tttatagatt ttgtataaaa
     9901 taaagttatg ttattaattt ttttagaaat aaaactttta ctaggagaca gatatgagaa
     9961 aaaatagtat taatatagca ctaactgcag caattacagc aatctatgca gttttaacat
    10021 tatcattggg atttataagt tatggaccaa tacaatttag ggtagcagag attatgatgc
    10081 ttttggcatt tttagataaa ggatatatag taggtcttac attaggttgt tttttagcaa
    10141 atgtaatagg accatatggt gtacctgata taatttctgg aacattagcc acatttataa
    10201 gtgcaagtat gatttatatt actagaaagt taggtaaaca aaataaaagt actttagtta
    10261 tagcttcttt atggccaact gttataaatt cgattgtggt tggtctaatg ttaaatgtat
    10321 tctttgggct tccattaatt ctatctatga tacaggtagg ttttggagaa tttgtagtag
    10381 taacaattgt aggagtacca tttttctgct ttttaatgaa aaaatataaa aatgtcatta
    10441 atattaaatt ttaaatagtg tatagtaatt taattaattg agagtattat tagtgaaaag
    10501 aggtgtttat ttgtcgaagg taaaaagatt gactgatgtt tggatatata ttaatgataa
    10561 gcaaaagttt acaataaaag agttgtcaga ggaatttaat ctttcagcta aaacaataca
    10621 acgatattta aaagaattaa ataaaatggg acttccaatt caagcagaac aaggtagaaa
    10681 tggaggttat agggtgctaa ataatagcta tatacctcct gttatattta cagaaaaaga
    10741 agttatgatt atactatttg cacttaagtc acttcagatt tataattata agttaatgga
    10801 aatagaaatt aattctataa tgagaaaagt aagttatgaa cgtaaaagca gtataaaagt
    10861 aggtatagaa aatattaaaa gatacattgg ctttgtagct aatgatacta atgatggaga
    10921 ttttaagagt agtaatgtaa ctgagttttt tagagcttct tcagaatctc taattttaaa
    10981 tattaagtat aagtgtggta atcagatttt ggaaaaaaac gtatgcccta taggtatata
    11041 tttaaataag ggagcatggt tttcaccagc atatgattgt aaaagtgata aaataatatt
    11101 agttcatata gataatataa ttgatataaa aagaacagga gaaagtaaaa aaatacatat
    11161 aaacttggaa gaatggttac atgaagtata taacaattta tatggaaata gttcaactac
    11221 taaaaaattt ggtgaaatta tttatctaaa tgtattatta actaaagaag gtgtttctaa
    11281 aataaaggac agttcttgga atttagatat ggagctaaat gaagatggta gtggaataat
    11341 tagtacattc ataaaggttg aagatataga ttgttatgct tcgattttat tttttattgg
    11401 aaataatgct aaggttatag agccaaaatt tttgaataag ctactttatg ataaggccaa
    11461 tgagttaaga tatttttatg aagaaaatat ggtataatat ttagaaatgt tgaaattaaa
    11521 taattattaa ggagatatac gttataatgg aaaattttaa taagaatgct aaaataattg
    11581 gtattggagt tgaaggtatt aatataataa atgaagtaga agaaaaaata aaagcaaata
    11641 tggatataga aaaaataaac ataaatcaag agattgaaaa agaatatgtt agaagtcttt
    11701 tagatggtgt agatatatta tttttgatat attcatctga agataaacat attagagata
    11761 ttataaaagc tgtttcatat atgtcaaacg aaagaagagt tctatctata ggaatggatt
    11821 gttcagagaa agaaaataaa gaagatttag agcttgggag agagtttaaa atcaataatg
    11881 atagtatatt taaatttgtt gatttaatga atatcatggt agagtctatt tctgattctt
    11941 gcatgataaa tatagatatt actgatttaa aagaagctat tgtaggagat aaaggaatta
    12001 agtattcttt tgaagagttt gaagatacaa agtcatatag cgagatagct gacattttat
    12061 ttgacaggat ggaatatatt ggtgaggaat ttataagtaa gaaaggaatt gtatttgttg
    12121 aaggaagtcc agaattttct ataatggaat tgaatgattt aataagtaat attcagagca
    12181 aggtagaaga aagctatgaa gtaatatttt ctttatatat aaaggaaaat ttaaatggaa
    12241 atattagagt aggcttattg tataattagt atattttaga tattaaggag agtgtatatg
    12301 tctgaaaata taagaggtac agagtattgg ttaatggaag aattttttta tcaggaaaca
    12361 gacacaccac agatttttat agaagaaagc ataattgcac ttggatttga tataaaaata
    12421 aatttaaaaa aagtattcag tatggggaaa aaatcagaaa agtatattaa tgagaattgt
    12481 aatgataaag attttataaa aaagtttatg tcgattaaaa aaggagatta ttttttatat
    12541 aaagcatatc aaaatataga agaaaaagtt aaatgtaatg tgtctattgg catagttgaa
    12601 gaagattttg aaaatggata tgaattaagt catattttag ggcatacact accagtaaaa
    12661 tggatgcata attttgatac agtttcaaat tgtaaagtat atacaagtga attttataaa
    12721 ttaaataata atgatattag ttcatattta gaaattatta agtcacaaga taagaaatta
    12781 ttgagaaaaa gaatagaaga aagtgaaaat aataaatggt atttaaaaaa ctattattat
    12841 aaaaatgtag ataagtgtgt agatgatgaa gaattattta ttcagcccat taatctagaa
    12901 caattagaaa gcctaaaaaa tataaagaca gatgattata ttttattgta taatagcgag
    12961 aaagtaattt gtgaaaatga ccatacaatg aagagtctat ttggcgtatg tcaagttatg
    13021 gaagggttcg ataaagagaa agtaagtgct ttggatggtg gatatttttt tcctgttaga
    13081 tggatagagt atggaaagga gaatatagac ggaaatgcat gggttatatt tgaaaataat
    13141 agtatagttg aaaagtatgt aaaaagattt actaataaaa tgccattaaa tactatactt
    13201 tatggaccac caggaactgg taaaacatac aatacaaaac agatggttta caatctagta
    13261 agtgaaacca gtctaagaaa caaatactat acaggttact tagattataa aaacaatgta
    13321 gaattttgta catttcatca atcatatgga tatgaggaat ttatagaggg tcttaagtct
    13381 gatggtgaag gcaattttaa gccagaagat gggatattaa aggatatatc tatagaagca
    13441 tgttataatg cattgaaatt tgaaaaaaag atgagctttg agttggaaaa agatagcttg
    13501 actttagaaa ctataaaaga caggaaaaag aaattagtat tagaaaatat agaatatagt
    13561 gagagtttta attttgaaga atcagaaaac tatgttttaa taatagatga aattaataga
    13621 gggaatgtat ctaaaatatt tggtgagcta ataacattat tggaagatga taaaagactt
    13681 actaaatcaa atcaaactat tgtcaaacta ccatattcaa aggaaaaatt ttctctacct
    13741 ccaaatttgt atatagttgg aactatgaac acatcagata aatcaatagc acttttagat
    13801 atagctttaa gaagaaggtt taattttgaa gaaattatgc cagattatga tttattgtct
    13861 attgtagatg gtatagatat tagaaaaatg ctatatacaa tcaataaaag gatagagttt
    13921 ttatatgata gagaccatgt tattggacaa gcttatctac ttgctgtaga aaatattaat
    13981 gatatagtag tagtatttag aagtaaaata ataccacttc ttcaagagta tttttattat
    14041 gattcagaaa aagtaggttt gattttagga ggaattggaa gaaatgaaaa agataaatac
    14101 attgtttata aagaaaaact tatagcttct gagttattta aaaataataa tgcctcaata
    14161 atggagagta aagttaacta ttgtataaaa caaaacatat ctagtgagga gctaaaaaat
    14221 atatatgagt aaaaatatat atgttaagga aacctatgaa tggataaggg taggtaatgg
    14281 tgaaaatgag cttacagaaa ttgaatatga aaaattgctt aaatatctag agaacaataa
    14341 tgatgtatta aaaagtaata taatagatat taaatacaaa aaaattagat ttataaacta
    14401 tgtaggcatc atatgttttg aaaatgtaat acttgaaata cttcctaaat tgtcattaag
    14461 tgataatctt gtaaaagata gagagatact attacagatg ctttctatat gtaataaaat
    14521 tccaattaca atgaatgaaa aaataagatt gagtttgaaa aattataatc tcttaaattt
    14581 ttttgttatg tatttcattg agagtatgca aacacagatg aaaaaaggta tttattttga
    14641 atatataaat aaaatagaga acttaaatgt tatgaggggt aaaatattat taagtacata
    14701 tgcaaaagaa aaaggcattt ctccaatgaa aataagatgt gaatatgatg agtgtagtga
    14761 aaataatttc ttgaatcaag ttcttaaaaa agcatgtata tctattttat gtagaataaa
    14821 tgacaattca attcaaggca agattaaaaa aattttaagc tattttcaaa atgtagattt
    14881 aatttatata gatagaaaaa aactgttggg ttataaattt tataaaaaca atgatagatt
    14941 taaagattgt tatttactag ctagacttct acttttaaat ctatctatgg ataatagtca
    15001 aaataatcaa gaggcatttt ctatattatt tgaaataaat actttgtatg aagaatatat
    15061 aggaatttta atcaaaagta tatgggacaa ttcatttaga gaaacatata tacaagataa
    15121 gagtaaattt cttttgaaaa atgaacaaac aggtaagaaa aactttaatt taagaccgga
    15181 tatagtttta tatgacttaa aaaatgaata tgaaattata attgacacta aatggaaagc
    15241 tattgaagta gattcaaatg ttttttatag gtcaagtgat atataccaga tgtatgcata
    15301 tataacagca tatgaaaatg caaaaagatg tatattatta tatccttgta ttcaaaaaga
    15361 taagaattat agttcctgga aattatcaga atcatttaag ggtaagttta
//
LOCUS       DAGQWR010000102        15043 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.102, whole genome shotgun sequence.
ACCESSION   DAGQWR010000102 DAGQWR010000000
VERSION     DAGQWR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.102"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(546..1904)
                     /gene="rlmD"
                     /locus_tag="MIW95_002733"
     CDS             complement(546..1904)
                     /gene="rlmD"
                     /locus_tag="MIW95_002733"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBY3152045.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAEGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(2079..3839)
                     /gene="pyk"
                     /locus_tag="MIW95_002734"
     CDS             complement(2079..3839)
                     /gene="pyk"
                     /locus_tag="MIW95_002734"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBY3152046.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEILTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(3891..4850)
                     /gene="pfkA"
                     /locus_tag="MIW95_002735"
     CDS             complement(3891..4850)
                     /gene="pfkA"
                     /locus_tag="MIW95_002735"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBY3152047.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(5059..8628)
                     /locus_tag="MIW95_002736"
     CDS             complement(5059..8628)
                     /locus_tag="MIW95_002736"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBY3152048.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(8785..9732)
                     /gene="whiA"
                     /locus_tag="MIW95_002737"
     CDS             complement(8785..9732)
                     /gene="whiA"
                     /locus_tag="MIW95_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBY3152049.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(9749..10174)
                     /locus_tag="MIW95_002738"
     CDS             complement(9749..10174)
                     /locus_tag="MIW95_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBY3152050.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(10243..11349)
                     /locus_tag="MIW95_002739"
     CDS             complement(10243..11349)
                     /locus_tag="MIW95_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBY3152051.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(11361..12218)
                     /gene="rapZ"
                     /locus_tag="MIW95_002740"
     CDS             complement(11361..12218)
                     /gene="rapZ"
                     /locus_tag="MIW95_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBY3152052.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIDEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(12249..12962)
                     /locus_tag="MIW95_002741"
     CDS             complement(12249..12962)
                     /locus_tag="MIW95_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBY3152053.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYNDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFKLA
                     MKTISDSNLDISLVENIII"
     gene            complement(12966..13880)
                     /gene="murB"
                     /locus_tag="MIW95_002742"
     CDS             complement(12966..13880)
                     /gene="murB"
                     /locus_tag="MIW95_002742"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBY3152054.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(14153..14923)
                     /locus_tag="MIW95_002743"
     CDS             complement(14153..14923)
                     /locus_tag="MIW95_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBY3152055.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         4524 a         2945 c         1624 g         5950 t
ORIGIN      
        1 aaaactatgt agttttaatt ataaaaaaag atgaacaaaa attatacagt ttaatatgga
       61 atcagtaggt acatatgtat tattaagaat tataaataat aaaaataaat tttagtcaat
      121 ctacaaaaac cttttaaatt gtctggaaat cacctcctca tttgttataa aaacatacaa
      181 caaatgaaga ggtgatttta tggtttttaa aaaaataatt ataagtggat atgtgctttc
      241 acatgtttat caacatttgt aatgcctata tttgctaatg aattataata taaaaatatt
      301 aaataatatt caactataga ttatgtaaat tatcctatga aaaaaataag ttgtataaaa
      361 tctggcaact gtagattgac gatttctttt aaaattacaa ttataataag ttttatagaa
      421 ttaaaaataa aactttagag atttttgata acattgtaga ctaaatttgc caccgaaatt
      481 actttacagt ggcaaatcgg tggcaaatac aatatattat tagtatataa tctatataaa
      541 cattactatt gcttgattat cttagctaca gtttccacat gtgtagaatg agggaacata
      601 tctacaggtt gtgcctcaac acatttaaat ccgtgaacat ttagatagtc taaatccctt
      661 gctaaagtag aaggattaca agaaacatag acaattcttt ccggattcat tgaaacaata
      721 gtctctaata ccttttcgtc acatcccttt ctaggtgggt ctacaactac tacattggca
      781 gtcttaccct ctctatacat cttaggtaca atttcctctg cctttccaac ataaaattca
      841 gcattatgta tattgtttaa tttagcattt atcttagcat cttttatggc atcatctata
      901 atttctattc cataaacctt tttagccttt tgtgccaaaa ataaagatat tgtacctatc
      961 ccacagtaaa tatcaaaaac agtatcagta tcatttaaat ttgcatattc aagagcctta
     1021 tcatacaata ctttagtttg gacagggttt acttggaaaa atgataatgg tgaaatctca
     1081 aacactaagt cacctatata atcgtttatt tttccatctc catatataac tatattttct
     1141 cttcctagaa tagcattcgt atctttttga tttacattta caaccaaagt cttaaatcca
     1201 ggtatatttt ccttaagcac tgaagctaat tctttaagat atggaagttt gtttccattt
     1261 gctactaata caaccattac ttctttagtt gtaaaaccta tttttgttac taaatgccta
     1321 atcaaccccg tgtgagtctt ttcatcatat atacttacct tataggccct tatatatgtt
     1381 tttattattt taacaatttt atcatttatc tcatgttgta ttacgcattt ttctgtcgat
     1441 attacatcat gagtcttctt tttatagaag ccaataattg gaataccatt attcttttgt
     1501 attggaaatt gtgctttatt tctatatcta tatggattat ccattcctat agtgtcatga
     1561 acttctacat cctctaactt acctattctc gaaagtactt gtttaacttc atttgttttc
     1621 atttcaagtt gtttattata atctaaatct tgtatctgac accctccaca atccctaaga
     1681 ttgtcactac acactctttt caccctgaat ggagactttt ctattatctc aacaatatct
     1741 ccctcagcat aatttttctt tactttatta atcctaactt taactttatc atgtattaat
     1801 ccgccctcta caaaaactgt aataccattg tactttccta ctcctactcc gccttgacca
     1861 atgtctacta tatctacaat atacatctcg tttttcgaaa gcataaatca ccctccaatc
     1921 aatctattat atttaaatct ttacaaactt ttttctttaa atttatatta aaatattgct
     1981 attaattaaa ctatattttt tactaagcta caatatttta ttttaccaaa aaaaaaggag
     2041 cttataaagc cccttagtaa ataaatttat tatttttctt ataaaactct agttgaacct
     2101 ctatatacca cgcctcttga tgcatccaca gttacaactt ctccatcttt tactagattt
     2161 gttatatcag ttgcagacac aataactggt atatctaaat ttatacctac aatagctgta
     2221 tgtgatgtca ttccaccatt ctcagttact acagctcctg ctttttctat ataattgttc
     2281 atttcagcat cagtagcagt agaaacaagt atgtcgcctt cattaaagtc tacacaagca
     2341 tttccattct ttattatacg aactttacct tctaccgttt gagtacctac acctatacct
     2401 tgcacgattt cttcgcttat tacatgaacc tttattaaat tagtagttcc acttactcct
     2461 actggtactc cagcagttat aactactaat tccccattat ctatataatc agcttgtcta
     2521 gctgcctcaa ttgccttttc tataacttca tctgtatttc ctgcaacaga agacttaact
     2581 ggatatactc cccatgtaag agctaattta ttcatagtct tttcattatt tgtagtagct
     2641 attattggag attttggtct aaatttagat accattctag cagtatatcc tgaagaagta
     2701 gatgttatta tagctgatgc atttaaatct actgctgttg tacaagttgc atggcttatt
     2761 gcatctgtta cagtaacatt gttagtacca ttttccttta gtaatctatc ataatctaaa
     2821 gtctcttctg ttctttttgc tattgtagcc atcattttta ctgcctctac aggatatttt
     2881 ccagcagcag tttctccaga taacattatc gcatctgttc catcatatat agcattggca
     2941 acgtctgtaa cttctgctct tgttggtctt ggatttctta tcatagaatc aagcatttga
     3001 gtggcagtaa caactggctt tgctaattca ttacattttt ttatcatcat tttttgaact
     3061 attggcatct cttcagttgg tatctcaaca cctaagtcac ctctagccac cattatacca
     3121 tcagaaactt ttaatatttc atctaggttc tctacacctt cttggttttc tattttagat
     3181 attatttgta tatcagttgc attattgttc tctaatactt ctcttattgc tagtacatca
     3241 gatgcctttc ttacaaaaga agcagctata taatctattc cttgacttat tccaaactct
     3301 atatcactta tatctttagg agttattgct ggtaaattta tcttaacacc aggtacattt
     3361 actcctttat gatttttaac tattcctgaa ttttcaacaa cacaaactat atcttctcca
     3421 tttatttctt ttactcttaa gccaactaaa ccatcatcta ttagtataga atctcctgat
     3481 ttaacgtctt cagcaagacc tttatagctt actgtacaca tttctttagt ccccataata
     3541 tctttcatag ttatagtgaa cttttgccct tcttctaaaa atacctctgg gtcttcaaag
     3601 ttacctgttc taatttctgg acctttagta tctaataata tagcaactgg cttatttaat
     3661 ttttctctaa ctttctttgc catatcaatt ctttctttat gttcttcatg cgaaccgtga
     3721 gaaaagttaa atctacacac atttagacca ttttgcataa gttgagttaa aatttcctca
     3781 ctttgtgatg ctggacctag tgtacaaaca atttttgtct tctttacatt atttaacata
     3841 tttgctcctc ctgagatatt attccacaga tttattttta atactaaaac ttatatagat
     3901 aatatttttg ccatctcata agcttcttta tcaaatactt tcttttgagc taaagcttca
     3961 cttatttcta aatcaattat tttgttttct tttattccta caactctagc tgattttcca
     4021 tctaataata attctactgc tcttactcca agtctacttg ctagtattct atcagataca
     4081 gttgggcttc ctcctctttg gacatgcccc aatacagtga ctcttaaatc tgctcctgac
     4141 tcttttttaa gttccttttc taaatcacta gcacttccga ctccttctgc taaaacgatt
     4201 atactatgtc tcttacctct tttttgagta gtctttaatc ttagagcaac ttcatctact
     4261 gtaatttcta cttctggtac tattatagtt tccgctcctc ctgcaagacc tgcatataaa
     4321 gctaaatctc cacagtgtct tcccataact tctactatgt taactctttc atgagatgat
     4381 gatgtatctc ttatcttacc tatagcatct ataatagtat tcattgcagt atcaaatcct
     4441 atagtataat ctgtatatgc taaatcgtta tctatagttc ctggtatgcc tatagctggg
     4501 aatcctaagt cactaagttt ttgggctcct gcaaatgaac catctccacc aattacaact
     4561 aagcaatcaa ttttatactt tttaagaatt tttactgctt ttaatctacc ttcttctgtt
     4621 ttaaattctt cacatctgga agattttaat atagttcctc ccctgtgtat aatatctcca
     4681 acagaagata agttcatttc agttaaatct tcttctagta atcccttata tcctctattg
     4741 attccatata ctttgcatcc ataatatatt gctgatctta caaccgctct aatcgcagca
     4801 ttcattcctg gagcatctcc tccacttgtt agtaatccta tagttttcat tttttacctc
     4861 cttggatttt atgtcaattt tttattataa tcattgttat tgtattctaa tggacttttt
     4921 ttgtccctat atcacttttt aatcccatca agactaaaaa aattaaatgt ccttaaatgt
     4981 ccattttgtg atattatatc acaaaacgga caatattttt catattatta taatatattt
     5041 agatttttat ataaaaaatc aagcttttac ttttatgtta tctttaggaa gtaactcttc
     5101 taagttattt ataaattcat cacatataca agtcaatgcc ccggtcattt tatatgcttt
     5161 acgtttacca tttatatttt catccatagg gtacaagtat atactgtcat tacctggata
     5221 ttgtttggct atttctacaa tattattagt tgtatcagta tcagacatac tatctatttt
     5281 caaatacaat tttttaccac tttctggctt aatattttct acatttctag ttcttcctga
     5341 attgtagttt ctatctgcaa agtcagtatc atcatttata tcttttatct ctctagctat
     5401 aagttttgca ttttcgcctt cttttatact taatgttcct tttacatata ttatattatc
     5461 ttcattcaaa atgacattat acttttgtaa taattgagga aaaactatta tttctatagc
     5521 accatacaaa tcttctaatt caataaatgc cataatttca tttcgttttg ttgttttaat
     5581 agttttagtt acaatcattc cacccattat aacatctgtt tcattcattt gtaggtatga
     5641 ctcttcttcc tcttttaatg aatttaattt tccattatcc atagatgttt tttcttgtag
     5701 ttctttttca aattgtgata aaggatgtcc acttacatac attcccaaga cttctttttc
     5761 aaggttaagt ctctctcttt cttcatactc ttgcctttta ggtaaactgt atatttgttg
     5821 aaaatcacta tcttcctcac tatctccaaa agcatcaaat aaagaaattt gaccttgaac
     5881 atttttcttt ctatccatag atacactttc taataaactt tcataaccag ccatcagact
     5941 agctctattt tcacttatat tatcaaaagc tccacatttt attagacttt ctattactct
     6001 cttatttgta tctttttggt ctaatctttt gactaaatct actaaatcta caaataatcc
     6061 attagattct ctttcagcga ttatattgtt tattatattt acaccaacat tttttacagc
     6121 tgctagacca aatcttatat tgtcgccttc aactgaaaac tttgaaaaac ttttgtttat
     6181 atctggaggt aatacatcta tttctagtgc tttacattct cttatatact caactacctt
     6241 atcagtattt cccattacac tggttatcaa tgctgccata aattcaactg gataatatgt
     6301 ttttagataa gctgtctcat atgctaaaac cccatatgca gctgcatgtg atttattaaa
     6361 tgcatatcta gcgaagtcta tcatatcatc aaatatttta ttagctattt cctcttctac
     6421 accatttctg atacaccctg caatttctat attaccatct tcatcaaatt taccatgaat
     6481 aaaatattgt ctttcttctt ccatcacgtc catctttttc ttactcatgg ctcttctaac
     6541 taggtcactt cgtccgtaac tataaccacc taaatctcta acgacctgca taacctgctc
     6601 ttgatatacc aaacaaccat atgtaacttc cattataggc tttaattttt catgtagata
     6661 tgttacatct tttgggttgc ttttattttt tatatatgca ggtatagaat ccattggacc
     6721 tggtctaaat agggatattc ccgctacaat atcttcaaaa ttgtctggtt ttagctgttt
     6781 cataaaactt ctcatgccag cactctccaa ttgaaacacc cctagtgtat ttcctgatga
     6841 aagcatttca tatactttcg ggtcgtcata atccatcttt gaaaagtcta ttttttctgt
     6901 gtagtttttt ataactctat ttttttctat taaatcaaga gtatctctta taactgtaag
     6961 agttcttagc ccaagaaaat ccatctttaa aagcccaagt tcttcaagtg ttgtcatagt
     7021 aaactgtgtt gtgattgcat cttgatgttt gtacaaaggt acatattcat ctacgggatt
     7081 ttttgatata acaactcctg ctgcatgagt agaagcatgt cttagcattc cctctatctg
     7141 ttttgatata tttattattt cttttgtttc cgggtcttgg tcatacaatt ctttaagatt
     7201 aggatttgta tctagggcct tatctattgt cattcctaaa gcaaatggta tttccttggc
     7261 taccttgtca actttattgt agcctatatc caataccctt ccaacatctc ttattgcagc
     7321 ttttgcaccc attgttccaa aagttattat ctgggctaca tggtcatcac catattttct
     7381 ctttacataa tctataactt cttctcttct ctcataacaa aaatctatat ctatatctgg
     7441 catggatata cgttctggat ttaaaaaacg ctcaaataat agagagtact ttattgggtc
     7501 tatatcagtt atcttaagtg tatatgctac tatcgaacca gccgcactac ctctacctgg
     7561 tcctactata atattatttt ctttagaaaa atttataaaa tcccaaacaa ttaaaaaata
     7621 ctctacataa cccatttttt caattacatc taattcataa tttaatctct ctagtatctc
     7681 attgctagga ttatcatatc tctcttttaa tccattaaaa cacaactccc ttaaataagt
     7741 ttctggtgta tatccttctg gaacatcata cttaggaaga tgtatagtat taaaatcaaa
     7801 ctccacatta cacatatcag ctattttcac agtattgtca atagcctcta atgcgtatgg
     7861 aaataattct tccatttctt ctcttgattt taagtaaaac tcatcactac caaatctcat
     7921 tctatttggg tcatttaatg tttttcccat ttgaatacac ataagaacat catgtatttt
     7981 cgaatcttct ttatttacat agtgaacatc attagtagca actagtggta taccagtctc
     8041 ttttgataat tttactaaac cagagtttac ttctttttgc tcaggtaaat tgtgatcttg
     8101 aatctccaaa aagaaatttt cttctccaaa tatgtctctg tagtcaagag caaattgttt
     8161 tgctttttca taatttctat tcattaaagc acgtgctaca tctcctgcaa ggcatgcaga
     8221 aagtgctata atccctttgt tatgtttttt aagttcttcc atgtcaactc taggcttata
     8281 gtaaaaacct tctacgtatg aagttgaaac tatttttatt aaatttttat atccttccat
     8341 attcttagct aacagaacta aatgtccttg atatttgtca tagtttgggt ctttatctct
     8401 caatccacgt gcagctgtat agacttcgca ccctataatt ggctttattc cttcttttat
     8461 agctgttttg tagaaatcta taacaccaaa catacaacca tggtcagtta tagctattga
     8521 gctcatgttt agttccttag ccctttttat aagttttttc accctcgaaa acccatcaag
     8581 taaactatac tcagtatgta catgaagatg agcaaaatct ttgttcatta aatcacctcc
     8641 tgctttataa ttttaatttt accacaaata tatgtcctaa aacaaaaaca gagactttta
     8701 tctcaaaata ggaattaact tcaacatata ccattatatg ttataaatta atgcttattc
     8761 tgagattgtc ccctcaattt attgctatga tttttttttc aaatctgttg caatttcttc
     8821 tatttttcta agtctatgat tgactccaga ttttcctatt ggtggttcta acatctctcc
     8881 aagttctttg agagtcatat cctcatactc gattctaatc ttagctatct cttgtaaatt
     8941 ttctggcaaa ctgctaattc ctatagtctc ttgtatgagc ttaatatttt ctacttgtct
     9001 tactgctgca tttactgttt tagaaaggtt agctgtctca caatttacta tcctgtttac
     9061 attatttctc atttctttta ctattttagt attttgaaga cttaaaagag cgtggtgtcc
     9121 tcctataata ctcaacaaat ctgatatttg ttcactctct ttgatataaa caacgtgatt
     9181 attttttcta gccacaattt tactattaaa acctaaagag tttataagtt cttttagaga
     9241 atctgcaaac tcctcattat tagttacaaa ttccatatga tagttctttt ctgggtcgct
     9301 tatagaacct ccaccaagaa aagctcctct tatgaaagct ctcttacatc cttcatgctt
     9361 aattaagttc tctgaaattt tgtttatagt aaaaaagcct tctttatctt caagtatacc
     9421 taactttttt aataacattt ctgaacccat ttcgcttgtt accattaata cataactgtt
     9481 atttctcttt agcatttgat tcttatttac tgagattgta gtattgatat taaaattctt
     9541 ttttaataat ttaaatactt tacgggctat tgaatttaac tctgttgtta tctttagatt
     9601 tagcttctta tatccaacga tttgtatact tccccctgat ttaactagag ctgataattc
     9661 agctatatta caacattcat tcccagatat aactcttgct agttcatttt tggtttcagt
     9721 tgaaaaagac atatctatca cctattaact aatttttctt taaccttttt tttatttctt
     9781 gtaaggcaac ctttattatc tccttttcat catcatacct agctaggtct accactctat
     9841 ctagatggat aaatttagca tcaataaagc cttcctcttt ttgtggaata gtatccatat
     9901 tctttgcttg catcaaatac caaaaaactg ttttatgaac tgctctattt tcatcccagt
     9961 tttctttgaa tgtatagtgt atttcaccta aatatttcaa tatatctgct tttactccag
    10021 tttcttcact tacttcacgt aatgctgcct cttggttatt ttccccttct tctacttttc
    10081 ccttgggtaa cacccaatct ccattgaact tccttagtaa aagaatcgta ttgccaaaca
    10141 ggactacacc accagcacta acctcttctc tcatcgatgt tcactcctta aaactaacat
    10201 aatgataagg atttctacac aattttttaa aatccttttt tactaacttt tgttgtaatt
    10261 atgatttagg gctaactcaa taactatgtt agatatatac tttgcatcat gtcttatata
    10321 attatttttt atttcaatta aattcttctc tacggttttt atacccatct cttttaattt
    10381 atctttttgc tttttatcta acaagacttg ctctgctcca tcttgtttat ataagtcaag
    10441 catttcttct ggcaatatct cattatttgc gattacataa tctattaaat tctcatcagt
    10501 gtgctttact atagcattaa catgctctaa tacattgtac ccttctgtct cacctggttg
    10561 agtcattata tttgctatat atacttttgg agctacagac gatttaatag cgtctacaat
    10621 cccctctaca agtaaattgg gtattatact cgtatataag cttccagggc ccataattat
    10681 aatgtcagca tcgtaaatag atgcaataac atcctttaat ggtttgacat cttttggatt
    10741 taaaaaaatt ttatcaatag agcttttttg aatttttgac tcttcaggta tactagattc
    10801 tccatttatt atattgccat tttctaattt ggcaactaaa tttacatctt ccaaagttac
    10861 tggtaaaact cttcctgtta tagcaaaaat ttcacttaat ttatatacag ctttttcaaa
    10921 gtttccatac aacccattca tagcagccaa aaacaaattt ccaaaactct gaccttttaa
    10981 aagcccttct gtaaacctat attgcataac ttcattcatt gttggctcta tatttgcaag
    11041 tgctaataga cagtttctta tgtctcctgg tgggagcatt cctaagtctt ctctcaatac
    11101 acctgaacct ccaccatcat cagcaacagt tacaattgca gttatatttt gcgtagtgtg
    11161 tttaagacct cttaaaaata ctgattgacc agtacctcca cctatcacaa ccacatttgg
    11221 ttctctattt tcaacaatag acctttgaaa tagttttact ttttcatttt ttgctttttt
    11281 atatataaat aacgatacaa ttaatgcaat ccaacctaaa attcctacaa taataagtaa
    11341 tttagccatg tttgcctcca ttatcgcaac atagagtctc tatgtttctt tacaactcta
    11401 tatcccttat tagataaatc ctccgctata agatttgtta tagtaactga cctatgtctt
    11461 ccacctgtgc atccaacacc aattactaga tgttgttttc cttcatctat atattgaggt
    11521 actaaaaaat gaatcatatc taaaagtttc acataaaatt cttcactgat tttagaattc
    11581 ataacataat ctctaacttc tttatcatca ccagttttag ctcttaactc ttcaacataa
    11641 tagggatttg gaagaaatct tacatcaaat actaaatctg catcagcaag tataccatgt
    11701 ttaaatccaa aagatactac agaaatagtt aggtttggat tatcttcacc agaagaatat
    11761 attttcttta tttcttcctt aaggtcttta ggtttcatgt tagttgtatc aattatacat
    11821 gtagataact cctttaaagg ttccattatc tttctttcca tttttatacc ttctggaatc
    11881 tgcatatctt ttgccaatgg atgatttctt cttgtcattt tatatctctt taacaataca
    11941 tcatcattac agtccaaata taccatttca tattcataat tttctttttc taaataattt
    12001 aagctttcat gaagcgcttc aaaaaacttt ctccctctta tatctatccc tagagcaact
    12061 ttatcaatac ttgaatttgg ttgataacat aattctgcaa atttagttat caaagcagga
    12121 ggtagattat ctacacaata aaatcccata tcctctaaag ctctcattgt ctcacttttt
    12181 ccagatcctg aaagacctgt aactataaca aatttcattg ctaactcctc ccttttatct
    12241 tatgtttatt atattataat gttttctact aacgatatat ccagattaga gtcagatata
    12301 gtcttcatag ctagtttaaa atcaccaact ctttctggac aatgtgcatc tgagcctatt
    12361 ataaatttat tttctagccc ttttatttta tttaattgat ttacattcaa aaaagaatgg
    12421 ctactattta tttcaagtct agtatttgtc ttttttgctg tctttgctac ttcttctata
    12481 tacacatcgc ctttatctcc tggatgagta attataaaaa tatcattatt acttatcgca
    12541 tttattacag cttttgtatt gtattctttt gctttttcag ttttaaaaat atagttatta
    12601 caatgattta ataagcttct taatgatgtt ggagtagaac caaaatgata tccagccata
    12661 atataatcaa aatctctaat aaatttatca tccatatcta tattgccttt atcatctaat
    12721 atgttacact ccatacctaa aagtatattt atatcattgt acttatcatt taaataatct
    12781 acttcttctc tcattttgag aatatctttt ttctttacac catatgcaat atgtttatag
    12841 ctatggtcag atattcctat tgtttttatt cctttagaaa tggctacctt tacattatct
    12901 tcaatagtcc ctttgccatg actatataat gtatgtgtat gataatcagc cagtattttc
    12961 atttgttaat cctctccaag tattttgact tcttcttcca acataacccc aaacttggca
    13021 tatacagcac tttttataac atatattaaa tccaaaatat ctttagcact tgcttctcct
    13081 tgattgacta caaatccaca atgcttctca gatacttgtg ctcctcttaa agtaagacct
    13141 ctcaatccag tttcttctat taatttacct gcaaaatatc ctgttggtct cttaaatgta
    13201 cttcctgcac tcgcaaaatt taatggctgt ttagttattc ttcttgtcgc taaatcatcc
    13261 attaaacttt ttatttcatc ataatttcct tcttgtaatt ctagttctgc tgataaagct
    13321 atatatccgt ttttcgatat tatacttttt ctatacccaa attccatctg ttcatttgat
    13381 aattcaaata tattgccttc catatccatc agtcttactg acttcactat gtccttcatt
    13441 tcaccaccat atgctccagc attcatggct agtgctcctc ccaatgtacc aggtattcct
    13501 gatgcaaact caaacccttt taattcttgc ttttggagag ctttacctaa aactgataat
    13561 aaagctcctg actgagcagt catcctagta tcatttatct cataagaatt gaaattatca
    13621 gctaattcta ttaccactcc tcttattcca ccatctttaa ttaaaatgtt tgaaccattt
    13681 ccaattatta aatacggaat agattcttct tttaccaact taagtacatt ttttaattgt
    13741 tcttcagttc taggtctaac taatatatct gctggtcctc ctaccctaaa tgaaatatgt
    13801 tttttcatag gttcatcgac ttttatatcc tctttactta atatatttaa taaatttata
    13861 tatatatctt gattattcat tgtatttcct cccaaattat actgaacttt agctgtcatt
    13921 attatttaaa ttacattagt atatagtatt aactatattc aataatatat gattttgctt
    13981 atcatatgga ttttatattt taagcttctt aattacaaac tatattcaat aaatatataa
    14041 taaagtaaaa aaaaaaaaat aacaacatat aaaagaatat aaataattta aaaaaagcat
    14101 caatatcact gatatcaatg ctttaaaagg caatttaatt cgtattattt atctaactat
    14161 ttttgacata gcaaagataa taaatccctc caactactat ggaacctcca acgatatttc
    14221 caagcgttac aaataccaaa ttagttataa attgaaccat agttatattt gctcctaatg
    14281 ccattcccat tggtataaaa aacatgtttg caacactatg ttggaatccg cataatacaa
    14341 ataacattat tactgtccaa catgaagcca attttgacat tatatcttga gatgctgtag
    14401 ccatccatac agctaaaacg actaaaatat tacaaagtat tgcttttaaa aacattgatg
    14461 ctatagttga attaacctta gcttctgcca cagctatagc ttttgtagct gcatctcctg
    14521 tcaatgtaga agaataaaac acaagtgcaa ctagcagaat tgaacctact aaatttgcaa
    14581 aataaaccag tccccagttt cttaacattt ttcctaatgt tatttttttg tccataagtg
    14641 caatagccat taaggtgttt ccagtgaata attctgctcc tgccaccact acaagcataa
    14701 gacctactgg aaagaccata gcacttaaaa agctagctac ccctgggtct atcttgccaa
    14761 aagtttgact aactatcata ctccccaatg ccccaaaacc tataaatgct cctccaaata
    14821 aacctaatag aattgcattt tgtgtcgaaa tgtttgcttt tttaatgcct gcacttatag
    14881 ttgcttgtgc agtttcccca ggtgtcaaga atcctttact cataaatcct tcccccttaa
    14941 aaattatact ttcccttgtc tttttatggc aataattata taataacaat atgatatata
    15001 ataaatttta ttaacataaa aaatatacta tatacttata tat
//
LOCUS       DAGQWR010000103        14436 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.103, whole genome shotgun sequence.
ACCESSION   DAGQWR010000103 DAGQWR010000000
VERSION     DAGQWR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14436
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.103"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..322
                     /locus_tag="MIW95_002744"
     CDS             <1..322
                     /locus_tag="MIW95_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBY3152056.1"
                     /translation="EKYNVDKSFSWDEYSKNSIGIYKGEEINVVIKISHPFSIIIKEK
                     VWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWILSMGAYVEVVEPDRLRNDILSEIEKM
                     KKIY"
     gene            400..1137
                     /gene="cas6"
                     /locus_tag="MIW95_002745"
     CDS             400..1137
                     /gene="cas6"
                     /locus_tag="MIW95_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBY3152057.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KAYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKIVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            1143..3020
                     /gene="cas8a1"
                     /locus_tag="MIW95_002746"
     CDS             1143..3020
                     /gene="cas8a1"
                     /locus_tag="MIW95_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBY3152058.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQIMFNDYLRQIIYFGELADLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTSINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLTNNILRNKVKNDMESNNKSNISGIDCFRVVQLRALINSYKKG
                     VYKMDSKNLEKNDEKLRIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            3022..3936
                     /gene="cas7i"
                     /locus_tag="MIW95_002747"
     CDS             3022..3936
                     /gene="cas7i"
                     /locus_tag="MIW95_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBY3152059.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            3938..4639
                     /gene="cas5b"
                     /locus_tag="MIW95_002748"
     CDS             3938..4639
                     /gene="cas5b"
                     /locus_tag="MIW95_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBY3152060.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            4650..6971
                     /locus_tag="MIW95_002749"
     CDS             4650..6971
                     /locus_tag="MIW95_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBY3152061.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQREFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIIGVGEANDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIHENKHTKQIFGKGVLISNEIDQFI"
     gene            6986..7537
                     /gene="cas4"
                     /locus_tag="MIW95_002750"
     CDS             6986..7537
                     /gene="cas4"
                     /locus_tag="MIW95_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBY3152062.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEMLIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKEKIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            7540..8520
                     /gene="cas1b"
                     /locus_tag="MIW95_002751"
     CDS             7540..8520
                     /gene="cas1b"
                     /locus_tag="MIW95_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBY3152063.1"
                     /translation="MYKDYYIISNGNIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNTLMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            8523..8789
                     /gene="cas2"
                     /locus_tag="MIW95_002752"
     CDS             8523..8789
                     /gene="cas2"
                     /locus_tag="MIW95_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBY3152064.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITDGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   8973..10457
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8973..9001
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            10647..11075
                     /locus_tag="MIW95_002753"
     CDS             10647..11075
                     /locus_tag="MIW95_002753"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBY3152065.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIEPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(11531..12310)
                     /locus_tag="MIW95_002754"
     CDS             complement(11531..12310)
                     /locus_tag="MIW95_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152066.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMSSEVSSIRCCNCDELLEDITLNKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            12634..13179
                     /locus_tag="MIW95_002755"
     CDS             12634..13179
                     /locus_tag="MIW95_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBY3152067.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            13211..14347
                     /locus_tag="MIW95_002756"
     CDS             13211..14347
                     /locus_tag="MIW95_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBY3152068.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CNLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIERADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     PILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKTKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIERYNIFGGALAYGH
                     PYGASGAIITLHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
BASE COUNT         5945 a         1337 c         2375 g         4779 t
ORIGIN      
        1 agaaaaatat aatgtagata aaagttttag ttgggatgaa tatagtaaaa atagtatagg
       61 aatttataaa ggagaagaaa ttaatgttgt tataaaaatt agtcatccat tttcaataat
      121 tataaaggaa aaagtttggg ttaataatca acaaatcata gaatatgatg ataaatctat
      181 aatgtttaaa gctaaaatga gaggttatga ggagataaaa agttggattt taagtatggg
      241 agcttatgtt gaggttgtag aaccagatag acttagaaat gatatattgt cagagattga
      301 aaaaatgaaa aaaatttact gattttttca aataatttat acttggacca atatacttca
      361 cggtctagtg ttaatatatt aattgagaga ggtgatatta tgaaaatgaa aattgaattt
      421 agtacaggtt gtataccaat atcttataat tcgctcttta tgagtattat aaaagaagca
      481 ataagaaaat ctaatgaaga ttactataaa aacatgtact attataaaga aaagaataat
      541 aaaaaaacta aaaattttac attttcggtt tatataaaaa agtatagtat tgaaggtgac
      601 aattttatta tagaagataa agtaatatta aatataagta ctccagattt agaattgggt
      661 tttcatatat acaatgggtt aatgacttct aaaaaatgtt tatataaagc ctatgaactt
      721 acaagaatta gaatcgattt atcaagagaa aagaaggtaa cagaagaaag ggtactattt
      781 aatgcattgt ctccaatctg tgtaaagtct aaagaaggaa aattcttgga gataactgat
      841 gatagataca tagaagaact aaactatata actaatgaag tggttaaaaa ttatagggga
      901 aatggattaa aaagaaagtt agaatttgaa aatataggtc tcaaaaagat agtagttaaa
      961 gagagcttaa gagaatttaa aaaaataaca ggtaaagagt atcaatatat taatggttac
     1021 aaaggtaaat ttatattgaa aggtgatatt gatgatttaa atcttatcta caatctagga
     1081 attggtttta gacgtgctca gggatttgga gatgtagata ttttagaatg gaggtgagat
     1141 aaatgaagtt aagagtttat agaggagatt ggttttttaa tatgggtata gttggatttt
     1201 taaatataat aaagaaagca gataaacaag ctgaaatatt tattatggaa gattatatcg
     1261 aatttgatag cttatttctt gaaaactttc atgagtatta ttttaattac tttatggatg
     1321 aatatgatgt atcaaaaaga attaaaaaaa atattgatta cagtataaat tttattaaat
     1381 ctaagcctga tagaatcaaa gatggaatta agaaaattaa agatagtgtg aaacaacaaa
     1441 atgataagat taaaaaattt gatgaagaaa aatttaacct gataaaagag aagctagata
     1501 gcatgtctaa gataaaaagt tatgatgaat ttgacagttt agaagcttta gtagatgaga
     1561 ctatagatat atttaaaata aaatcaataa atgacaggct tacagcaaat ctttataaat
     1621 atgtagttca agataattat tttggacaag ttagtttttt taatgttgct aaggcgaaat
     1681 tagatttaga tggattaaaa cagataatgt ttaatgatta tttaagacaa attatatatt
     1741 ttggagagtt agcagattta ttagaagaga atgactatga taagttgaaa aactatttaa
     1801 atgatagatt aaatagtata gctaaggata taaatgaaaa aaaagttagt aagtctagta
     1861 taaatactat agaaaaaatt atgaaggaaa taaataaaaa ctttataaag aaaaataaaa
     1921 gcatagaaga tataaaagag tatatggata gtttagaagt atgtgaaatg tgtggtttat
     1981 ataagggaat attagatgaa tactcagaaa gtaattttgc tccacttgga gtaagcacca
     2041 ataatgctag gaatatgttt tggaatcagg cgtatgtacc atcaatatgt gatatttgta
     2101 agctaatatt gttctgtact ccagcagggg ctacttatac aagaaagaat tatttaatta
     2161 atgaagaaaa tgagttttat ttatttgtta acatggatac ttctataaat gagctttttg
     2221 aaagaaataa tagtttaaaa gctcaaaaaa gtgaatattc agattccaaa gatgaaaatc
     2281 cttttaatca gcttataaaa agtatagtag aagaaaatac attaaaaagt gaatggcaac
     2341 ttcgtaatat actttttgtg gaatttaaag caagtataga ttctaaaaaa tgtaaaataa
     2401 attattttaa tattccaact tatttagcaa aattttttac agacaagtat gcaaataaaa
     2461 aaatacaaag tatctatgat tacaagctta aatcaaatgt actagattta ctcttgaaaa
     2521 atagggactt gaagcatcta actaataaca ttttaagaaa taaagttaaa aatgatatgg
     2581 aaagcaacaa caaatcaaat atatcaggaa tagattgttt tagagtagtc caattaagag
     2641 cattaataaa tagctacaaa aagggggtat ataaaatgga tagtaaaaat ttggagaaga
     2701 atgatgaaaa gctgagaata atttattatt taggttgtga tatacatgac tattttgtta
     2761 ataaaaattc taaaaataaa atagatggag taagctataa acttttaaat tcagttaaag
     2821 tcggaaataa aagtgatttt atggatacaa ttataagagt ttttatgtca gctgaaaaac
     2881 agattccagc atttatttta gatatagaaa ttgaaaaaga tttagacttt gaatctatag
     2941 gtcatgcttt tatatctgga ttaatttctg gaaaatatga aggaaaagac aaactaccaa
     3001 acgaaaagga ggaaaaataa gatgaaaaga gggttaacat ttacagtaat agcacaggct
     3061 caaagtttaa actatgggga agggataggt aatatatcag aattaaaaaa attaagtaga
     3121 gatgatggaa atatatatac atttgcatct cgccagtgtt taagatatga catagtacga
     3181 ttagcagcag aattgtttaa ttggaattta caggttgtcg ataaagaaaa aggtactatt
     3241 caatttaaag acaatataag tataagagaa tcagaagaaa tggatttatt tggatatatg
     3301 aaaacaaata aaaaagatga gaaagataaa aaaggaggtt ctttgactag agaagctact
     3361 gtaagattaa gtaatgcaat ttcccttgag ccatatagaa gtgacatgga ctttttaaat
     3421 aataaaggat tagcagatag aataggagaa catcctaatc ttgctaacat agagcaacat
     3481 ttaagttatt atacatatac agtgactata gatttatcta aaataggtaa ggatggagat
     3541 atagaacttg ataataaaga gaaatgtaga agagtagtag aatttttaga aataataaaa
     3601 gtattgaata gaaatattag aggtagacaa gaaaatttat ctcctctatt tgtagtagga
     3661 ggagtatatg aaatagcaaa tccatttttc ttgggtagaa ttaaattaaa aggagataaa
     3721 aatggattta aaataaataa gcaagctata gaagaagtaa ttcaaggtac ttttttagga
     3781 aaagatttaa aagaatttac ctatgttgga atggtagatg gggtatttat aaataaagaa
     3841 gaatttaagg gattgtttga agataatttt ttaagtgtag ataaattttt tggacaacta
     3901 gttaaagaag taaaggaata ttatggggtg aattaatatg aaggttttga aattagattt
     3961 atttcaagaa actgcatgct ataaaaaacc atttgctatg aagattacag agacatatcc
     4021 actgcctcca tattctacaa taaatggatt aattcacaaa atattaaatg ctacagaata
     4081 tataccattt aatataagtg tacagggaac atatgaaagt atatttaata attaccagac
     4141 aacatatttt tataaaaaag atagtattac atctatgcca atgaatagtc atatgctatt
     4201 gaatgtaaat ttgataattc atataggtgg ggattttgat ttattggaag aaatatatga
     4261 tagtttatta aattatgacg agcacttatc attaggtaga aaagaagatt tagtaagaat
     4321 taatgatatt agatttgtag aagtcaataa atttgaagta gatgaggata tacaattaga
     4381 tgattatgaa aattataagt tatcagaatg taatataaga atgcctatat atataccaaa
     4441 aacaagttta caagatacag atatagatgg tataagttac agactcaata attactatga
     4501 taataatgct gtggaagata aaaaaagggt ttggaataag gtagatagtt attatgttga
     4561 ggatggaaat actatatcaa ttggagatat attattagat aatgaaggag atttattata
     4621 ttttaataat ataaattagg agggagagta tgttatatgc taaatctaat ccagtggaaa
     4681 cacttagaga acatactgat gaacttttaa aacaaatgaa tgtattaaga gaaagttatg
     4741 gaaaaaatat aaatagctta gattttctag aggaagagat attttggcaa ttacttgact
     4801 ttgttataga atttcatgat attggaaaag cattttcacc gtttcaagag ttaataaaaa
     4861 gcagaatgga tactaaaata aatgaaccta aaatagtaac tcatttagaa aataatgtag
     4921 gccataatta tctttctcct gcatttattg attatagtta tatagacagg aaaaaaaata
     4981 aagaacttag agcagtatta aatcaagtta ttgtatatca tcatgaaaga gatatcttta
     5041 tagataaaga ttttaaaaat ttaatacaaa aaatattaga tgaagattta ataaataaag
     5101 tttatgaact acagcgtgaa tttaaagtta gatatccaat aaaaacagaa aaattgagta
     5161 aagtatatct acaaagtgta gaaaaaagaa tagataaaaa tcacaagtac tataatttat
     5221 atattatgtt aaaaggaata ctacatagac tagaccatag tgcttctgct catgaagttg
     5281 tggaatgcaa taatgttata aatataggag aacagacaga aaattattta atgaaagagt
     5341 ttggtagttt gagagaggct caatcatttg ctaaatctaa tagaaataag aatataatac
     5401 tgattgcatc tacaggaatg ggaaaaacag aaactgcttt gatttggata gataaggata
     5461 aagcattttt tacactacca ttaagagtaa gtataaatgc attatttgat agggctaaaa
     5521 atataattgg agtaggagaa gcaaatgata cattcttagg tctgttacat tcaacagcaa
     5581 tagattattt agaagaaagt aatcaggaaa attcaagtga gattgtagat ttagcaaagt
     5641 tattatcttg taaactgaca ttttccacaa tagaccaaat atttaaattt ccttttttgt
     5701 atagaggcta tgaaaaagtg tactcaacat tagcatattc aaaagtagta atagatgaga
     5761 tacaagcata ttcaccagaa atagcagctg tattggtaaa aggtatagaa atgatacata
     5821 agataggtgg cagatttatg ataatgacag ctactatgcc aaccatatat atagatgaat
     5881 taaaaaaaag aggtgtaatg aatagtaact tagctgactt gacatgtaac acagaaaaaa
     5941 taagacattg tgtgtctatt gtagaaaatt caatagatga aaacttaggt aaaataatac
     6001 aaagtggaat gaataaaaaa gttcttgtaa tagtaaatac agttaaaagt gctgtagaaa
     6061 aatatgagtt aatagaagaa atagttaaac caaaaggaat agatgtcaac ttaaatcttt
     6121 tacattctat gtatatacaa gaagatagag caaaattaga aaaatatata aaagaatttg
     6181 cagatagtga tagtaatggt atttggatta ctacacaatt agtggaagca tcccttgata
     6241 ttgattttga tgagcttcat acagaaaatt caacacttga cagtctattt caacgatttg
     6301 gtagatgtta tagaagtcgt gagtatgaag aaaatagccc aaatatattt atatatacag
     6361 aaaaagctac aggaataggt agtatttatg ataaggatat tgtagaaaaa ggacttgagc
     6421 tactgaaaac ttttataaat ggaaaagaat gtgtgaagat gaaagaaaaa tataaggtag
     6481 aaatggttaa gatactatac tctaaagaaa gtctagaagg tacagctttt gaaaagagat
     6541 ttaccagtgc tttaaatatt ttagatacta taacgccata tagtttaggt tctaaagatg
     6601 cacaaaatat acttagaaat attgaaggat atacagtaat tccagaggat atatataata
     6661 aaattgaaga aactttaatc aaagattatg aagagttggg acaagagctt ataaatgcat
     6721 atagtaatga tgatagacaa ttgataaata aaataaagtc taagagaaaa aaagcaagaa
     6781 gagagataat taaaaaaact gtaaatttac caatatataa agctaaacaa aatgtaattg
     6841 acatacaggt gaaaggtctt gaagatttaa aaatactttt atataaatat gatatacacg
     6901 aaaataagca tacaaaacag atatttggta aaggtgtgct tataagtaat gaaatagacc
     6961 aatttatttg atttttatat taacaatgtg gctaaaattt actaaagaag gtgattttgt
     7021 gccaatagat ttagaagtat taaaagttca aggcgtaaaa ataaattatt attatgtatg
     7081 taaaagaaaa ctatggctat tttctaaagg aataactatg gaacaaaata gtagtagagt
     7141 attgtctgga aagatagtac atgaagattc atataaaaga atgaaaaata aagaaatgtt
     7201 gattgatgat atcttgaagc tagatataat aaaggacgag tatgtaaaag aagtaaaaat
     7261 aagttcaaag atgaaagaag cagatgaaat gcagttatac tattacttat actatttaaa
     7321 gaatagtatg ggtatagaga aaaaaggaac tataaattat gttaaggaaa agaaacaaga
     7381 agaattgata ttgacagaag aaaaagaaaa aattattgaa aacacactta taaatataaa
     7441 tgaaataaat atgcagttat ctcctcccaa aattgagaaa aaacattatt gtacaaaatg
     7501 tgcttattat gaattttgtt ttgtgaagga ggagtaacaa tgtataaaga ctattatatt
     7561 ataagtaatg gaaatataaa aaggaaagaa aatacgattt actattacaa tttagaaggt
     7621 gagaaaaaaa tattaccagt agaacaagtt gatgatattc atatatatgg agaagtagat
     7681 ttaaacacaa aacttataaa ttacataagt aaatatgggg tgatgcttca tttctacaat
     7741 tactatggat attattcagg tacatattat ccaaggaaga aaaatgtatc aggattcagt
     7801 ttagtatgtc aatcagcctg ctatttagat aaaaatgaaa gattatactt agcaaaatcc
     7861 tttgtggaaa gtgcatcata ccatatttta agaaatatga gatattacaa agttgatgaa
     7921 agtttaattg atactataaa ggatgaacta aatgatttga atgatgtcaa agaaatatca
     7981 gaacttatgg gtaaagaagg cagaataaga aaaacatact ataaatcatt taatcaaata
     8041 ttaaaagatg attttgaatt ttataaaaga gaaaaaagac ctcccaaaga cccagtaaat
     8101 actcttatgt cttttggaaa tagcataatg tatacgaatg ttttaagtga gatatataag
     8161 acacaacttg accctacaat aagcttttta catgaaccat caacaaagag attttcttta
     8221 agtttagata ttgcagaaat ttttaagcca attatagttg acacaataat ttttagcctg
     8281 attaataata aaagaattaa taagaatgat tttgaatatc aagacaaaat ttgttattta
     8341 agtgaaaaag gaaagaagaa atttttgcaa gaatttgaaa ataagatgca gacaactata
     8401 aaacatagaa atctaaatag aaaagtttca tatagaatgt ttataaggtt agagtgttat
     8461 aaattaatta aacattttat aaatgatgaa aggtacaaag ttttaaaggt gtggtggtaa
     8521 gtatgtttgt tattgttact tatgatattg ttgaagcaag gtcgttaaat agaattagaa
     8581 ggatacttag aaaatatttg acttggacgc aaaattctgt ttttgaaggc aatattactg
     8641 atggaaagtt acataaatgt atttctgaaa tagaaaatat tattgataat agcgaggatt
     8701 caatctatgt ttatgagata aaaaatccta attcaattaa aaagaaatgt tatgggattg
     8761 ataagtattc tgatgaaatg tttatatagg tttgcagtga gcgatattta tgctaaaata
     8821 ggtgttaaca gttggaatat aagggattga aggtgtatga taactgttat caattgcact
     8881 actgctcgct cactgcaaat tttgatgttt ttattgaatt ataattgctt gattgaagta
     8941 ttttcaatgt attcaattat acctattttg gggttttata ttaactaagt ggtatgtaaa
     9001 tttaaaggtg caagtcttaa tacttgttac atgagttgtt ttatattaac taagtggtat
     9061 gtaaataaca aagaaccatt tgaatcacga aaactaaaaa acagttttat attaactaag
     9121 tggtatgtaa atttattaac cttatcagaa acctgaccat cccaattata gttttatatt
     9181 aactaagtgg tatgtaaatt ccatttgtct aatgcttcta actctttttt tatacgtttt
     9241 atattaacta agtggtatgt aaattccaaa atgggcttgg agttaagagt tgtataagaa
     9301 acgttttata ttaactaagt ggtatgtaaa ttaattacac actccctttt aacgaacata
     9361 ttttagattg ttttatatta actaagtggt atgtaaatag actcgacata gaatcggtta
     9421 ctaaatccga gggtttcagg ttttatatta actaagtggt atgtaaatac taaacctttt
     9481 tgtttacttt tatttctcat tttacgtttt atattaacta agtggtatgt aaatccggat
     9541 ttttctgctt cgactgttgt tattgagact tgttttatat taactaagtg gtatgtaaat
     9601 agtaattcca tgcaatcttt tatattgctt tttaacctgt tttatattaa ctaagtggta
     9661 tgtaaataga tatacaaggt gaaagtgttg aaatttcaat cttgttttat attaactaag
     9721 tggtatgtaa atattcttag atgttataat ttgtgttgca tttaaatcgt tttatattaa
     9781 ctaagtggta tgtaaataca atattattgt tcatttcatt taataattct agtagtttta
     9841 tattaactaa gtggtatgta aatttgttta tacctttaat ctttaaaatt aaatataaaa
     9901 gttttatatt aactaagtgg tatgtaaatc attgtatact aagcttgata tagtgcaaaa
     9961 tgaaacgttt tatattaact aagtggtatg taaatactaa ttgggttaaa caaatggata
    10021 taactaaatt agttttatat taactaagtg gtatgtaaat ccaacccttc gcacgaatcc
    10081 gtgcgacgaa atataaagtt ttatattaac taagtggtat gtaaattgta aaaatattgc
    10141 ttagcatatt gatatcacta actgttttat attaactaag tggtatgtaa atacaagagg
    10201 tttttcagca atatatgtac ttctataatg ttttatatta actaagtggt atgtaaattc
    10261 atcaatatta attttgggaa tatcaacatt ttttgcgttt tatattaact aagtggtatg
    10321 taaatgcgat aagagattcg agtcttttta ctgggcttaa tcgttttata ttaactaagt
    10381 ggtatgtaaa taggataaag aaaagactca cacaagacac agtgtcaggt tttatatcaa
    10441 ctaagtggta gataagtaga atattagcaa tatcaacgag tatttagaaa cttattttat
    10501 attaataaag cagtatataa aattgtagaa taaacaatag catatactaa aacatccacc
    10561 ttatattaac taaatcatat ctaaataaaa tgcacttacc tataaacatt ataaaatcaa
    10621 tacaaaaatg aggtgaaaca aaatttatga taaagaaatt aaacaataaa gacataaata
    10681 aaatcatgga aatatgggaa aaaagtacaa tcaaagcaca tgactttata agtaaagaat
    10741 actggcaaaa taactacaat actgttaaaa acgaatatat acctatatca gatacatttg
    10801 tatatgatga tggagatgaa ataaaaggat ttataagcat aatagataaa agctttatag
    10861 gagctttatt tatagaaccc aaataccaaa atctaggtat cggaggtaaa cttttagatt
    10921 atgcaactaa aaaatataaa agtctaagtt tagcagtata taaagataat aaaaaagcag
    10981 ttgtgtttta taataaaaaa ggttttaata tagtaaaaga acaagtaaat gaagattcag
    11041 gatttaaaga gtacataatg gaatatagta aataatatga ttacattcta ttaaaagaat
    11101 aattaattta ttttctattt gatttttata agagtaattt ttaaaataga ctatatgaag
    11161 ataaagatat ttattataga tgtaagttaa tcagaattga tatagctaca aaagaggaaa
    11221 ataaaattgt atggtgtatt tcagatttaa caaatttact atttaaagat aataagtttg
    11281 atataatatt aaagatactt tcacctataa actatagggt atttgaaaga attttgaata
    11341 attatggaat gctaataaag gtttgttaaa atttaataat tattagatta tagaaaattg
    11401 ccatgaaatt agttcagcaa gaactagttt catagcaatt ttaattctta taagtgtatt
    11461 tatttttaca gatttaaaaa acttatacag tatctaataa tgtaatctta ttactttagc
    11521 ctataaatat ttaaatctta aaatgttctc tttgagataa gtttatttca gagtcacaat
    11581 ttccacaaca gtaagatgtt gagacatctt taaatttttc agtattctca tcaaagttca
    11641 tttttttctt tgcaataatt cctaccttag ttaaattaga accacaatta ttacagtttt
    11701 gaattttaca taggttatat agtttattaa gtgtaatatc ttctaataat tcatcacaat
    11761 tacaacatct gatagaagaa acttcactag acatttcaat aggttcagct tctttagaag
    11821 aaaatttata tgttatgtca tttgatactt tataacctgt atcaaatata ttagaattac
    11881 aacttggaca taagtacttt ggaagatata tatctttttt ttcaaaagta tcactactaa
    11941 tattacagtt taattcatct aaaattggtc tttcatctag gttattacct ttatttaagc
    12001 ttagaagata attataaatt tttttatctt cattctgttt tgatgtatct aacaaatgct
    12061 ccattatatt agtgtatttt ttgttttttg aaggcatgtt taaatccaga taattgtttt
    12121 caatagcaaa gcatactata ttgaaatagt tatatttaac tgcaaaaaat aataacattt
    12181 catcaaatac tttatctatt attttgtcag aaatacttat ccaaaaatct ggtgactcta
    12241 agtaaacttt cctactaagt tcatctaact cttcatctat attatttata ttattttcat
    12301 taactttcat atcatcactt ccttttaaat atcttgtgta taaactattt gttactattt
    12361 tttatataaa ataaaatctt atataaaaat atatcatatt ataatttaca agtatagtct
    12421 aaatagagct gtttttaagc aaagttttat aaatgactat taaaatataa agtaacagta
    12481 taaagtttac ttataaatta aacgtaaacc taaatacttg taatggttga caaaaaaacg
    12541 tctttatttt atactaaaac aaagctttaa tagaggcttt gtatatgtac atataaacta
    12601 aatgaaataa gacatatgaa gggaattata aaaatgaaaa atactaaagt aaatattcaa
    12661 gatttaacta aaatgtcaat atgtgtagca ttattgtgta tatcatctta catatatata
    12721 ccattgccat ttactccagc aggagtaaca gcacaaacaa taatgataaa tttgatagct
    12781 ttgattttaa caccaagaca agcatttagt acagttggtg tatatataat gataggttta
    12841 attgggattc cagtatttgg tggaggtaca tctggtattg gaaagttgtt aagcccaaca
    12901 ggtggttttt attttgggtt tttgtttgca gcactaatta ttagtttatt aaaaggacga
    12961 aataatgata ttaagagata tatactaata acaatttttg ttggaatgcc tattatatat
    13021 tttatgggaa cagtatttat gtgctatttt aatcaaatga cagtagaagc agcacttttt
    13081 gcagcagtag taccattttt aattggagat acaataaagt cggttattgc atctttttta
    13141 ggcactaaac tgaacaatgt actcagaaga aatctatgat aaatctataa tataaggaag
    13201 ggtgtaatgt atgaaggaag tttttatatt aggaggattg agaagtcata taggtctaaa
    13261 aaatggaatt tttcaatttg ttcaacctga attactagga gcaagtgttt taaaagattt
    13321 aattaaaaag tatgaaattg acaaaattga tgagattata tgtggaaatg cagttgggac
    13381 aggagggaat atagccagat taatgacttt aacagcagga gtgtcaaatg aagtacctgc
    13441 ttttactgtg gatatgcagt gtgcttctgc tatgatgagt atagacatag ctttttcaaa
    13501 ggttaaatct ggtcagtgta atttaattat tgcaggtggt tttgaaagta gttctctaca
    13561 accaatgcgc acatatcata agaatgataa gcgatataat acaaataatc caaattatac
    13621 agttgctcaa ttttctcctg atgataatag tcaaaatagt atgctcgaag gggcggaaag
    13681 agtagcagag ttatatcaaa ttgagagagc agacttggat ttttgggtaa aggaaagtca
    13741 taaaagagca aaagaagcaa gagaagaaaa aatacttgag gatattatat ctcctataaa
    13801 taatagtacc tatgatgaag gtatacgtga taagatgagt caaaggttat tagataggat
    13861 gccaccaatt ttaggaaaag aaactattac aaatgcagca aattcatgtc ttattaatga
    13921 tggagcttca tttattataa tatgttctaa gaagtattta gaacatgtaa aaaagaagcc
    13981 taaaacaaaa attattaata catgcacaat aggaacagat gcaaccttaa gtccaacatc
    14041 agcgatacaa gcaatggata aacttttaga gatagaaagc cttaattatt tagatgtatc
    14101 tgctgttgaa tttaatgaag catttgcagt tatagatgtg ttgtttcaaa gaaagtatcc
    14161 agagttaata gagagatata atatatttgg tggagcttta gcgtatggac atccatatgg
    14221 tgcttcaggt gcaattatta cattacatct tttaaaagca ttagaaaaaa ctaagggtag
    14281 atatggaatt tgctctatag ctgcagctgg tggtctaggt tcagctttac tgcttgaaag
    14341 ggtgtagatt atgaactatt atgaactttt aagaataaaa aaagagaggc ataaaaataa
    14401 aaatttttta attatagatg gagaaaaata tactta
//
LOCUS       DAGQWR010000104        14148 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.104, whole genome shotgun sequence.
ACCESSION   DAGQWR010000104 DAGQWR010000000
VERSION     DAGQWR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.104"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            190..2067
                     /locus_tag="MIW95_002757"
     CDS             190..2067
                     /locus_tag="MIW95_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY3152069.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            2379..3785
                     /locus_tag="MIW95_002758"
     CDS             2379..3785
                     /locus_tag="MIW95_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBY3152070.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            3813..4661
                     /locus_tag="MIW95_002759"
     CDS             3813..4661
                     /locus_tag="MIW95_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY3152071.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            5322..6014
                     /locus_tag="MIW95_002760"
     CDS             5322..6014
                     /locus_tag="MIW95_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3152072.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            6011..6946
                     /locus_tag="MIW95_002761"
     CDS             6011..6946
                     /locus_tag="MIW95_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3152073.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            7094..8017
                     /locus_tag="MIW95_002762"
     CDS             7094..8017
                     /locus_tag="MIW95_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152074.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAHAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            8001..8771
                     /locus_tag="MIW95_002763"
     CDS             8001..8771
                     /locus_tag="MIW95_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152075.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            8781..9563
                     /locus_tag="MIW95_002764"
     CDS             8781..9563
                     /locus_tag="MIW95_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152076.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            9940..10476
                     /locus_tag="MIW95_002765"
     CDS             9940..10476
                     /locus_tag="MIW95_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBY3152077.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            10508..10897
                     /locus_tag="MIW95_002766"
     CDS             10508..10897
                     /locus_tag="MIW95_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBY3152078.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            10934..11320
                     /locus_tag="MIW95_002767"
     CDS             10934..11320
                     /locus_tag="MIW95_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBY3152079.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            11447..12889
                     /locus_tag="MIW95_002768"
     CDS             11447..12889
                     /locus_tag="MIW95_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBY3152080.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVENESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLIDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            13186..14001
                     /locus_tag="MIW95_002769"
     CDS             13186..14001
                     /locus_tag="MIW95_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY3152081.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKRVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         5098 a         1735 c         2688 g         4627 t
ORIGIN      
        1 atgatactat aaagttaaca aatttgaata tataacagta gacgtgttac tggtaatgca
       61 ggcaagatcg aatcaatttt tatagattat ctatagagta ctatgggtat aatctattta
      121 tttggttcga tttttttgtt taattggcca taaaacaaaa agcctatagt tgaaaatgga
      181 ggatttttta tggattataa aaatatagct caagaaattc tactaaatgt aggaggaaaa
      241 gagaatgtaa atgaagtgac tcattgtatg acaagactta gatttaaagt caaaagcgct
      301 tctaaagtga ataaggataa attatctaaa actgaaggag taataactgt tgttgagagt
      361 atgggtcaaa tacaagttgt aattggaaat aaagtaaaaa cagtatatga tgaggttata
      421 aaaattgtac ctcaatcaaa tgacgtaggt aaaaagaatg aaagtcaaga aaagcaaagt
      481 ataattaatt ctatattatc agcagtagct ggtatattta cacctacaat acctgctata
      541 gcaggggttg gtatgattaa aggtatttta tcagttcttg ctatgtatta tatgaataaa
      601 aatggagtag atataaaaga aacgcaaagt tatattatct taaatgccat ggccgattct
      661 atattctatt ttatgcctat tatactagga tatactgcgg ctaaagtatt taatgcaaat
      721 aaaataatat ctatggtatt aggagctaca ctttgttatc caacttttac tgctttgatg
      781 gcaggtgaag aatctgttag atttttagga ttggcagtaa caaaagctac gtatacatca
      841 tctgttattc caataattat tgctatatgg gctctttcat acgttgaaaa agtattagaa
      901 aagtatatac ctgaggtaat taaaataata atggttccaa ctttatcatt ggttgtaatg
      961 ttacctgcaa cattattttt atttggacca atcggaattt atataggaaa tgtcataaac
     1021 ttttcttata agtatattta tgaattaagc ccagcgttgt gtggagcatt tgtaggtggg
     1081 ttatggtgtg tgttggttat atttggtgct catagagcat tattaccaat aggtataagt
     1141 gatgttgctc aaacaggaag acagaattta ttggcatttg caggagctgc aaacttttca
     1201 caagcagggg ctgctctagg agtattcttt aaaactaaaa atcaaggact aaaaacaatt
     1261 tctatgtctg caactataac agctttattt ggtataactg aacctgcaat atacggtgcc
     1321 aatcttagat taaaaaaacc tatggtatgt gccgtaatat gtggtgcatt tggaggtgcg
     1381 ataatgggaa tgggtggagc ttatggtaat gcatttgcaa accaaggtgt tttaactata
     1441 cctgtatatg ctgaagcagg tgcattaggt ttcttaagtt atctaggtgg ttgtgctata
     1501 gcattttttg gttcagctat ttcaacttat ctagttggat ttgaagattt ggaagagtct
     1561 tctaataagg aaaattcatt tataaaagta gaaacgaagg atggtgttat tgatataact
     1621 tcacctgtag agggcgaatg tatagaattg tcagaagtaa aggatgatgt atttgcttca
     1681 aaagctatgg gagaaggtat agctgtgtta cctacaaaag gagtaataac agcccctact
     1741 gattgtgaag tagcttcttt atttccaaca ttacatgcaa taggattaaa attagataat
     1801 ggagcagaaa tgttaataca tgttggaatc aatacagtag agttaaatgg taaatacttt
     1861 acaaaacatg ttaatcaagg agatttagtg aaaaaaggtg ataaacttat atcgtttgat
     1921 atagataaaa taaaaaaggc tggttatgat gtaactacgc cagttatagt aaacaataca
     1981 tttgattttg ggcaagtagt ttcttgtaaa tctagttatg tttcaaccaa tgataatata
     2041 atttcattag tattagctgg taattaagtt aatttgacta gagataagta aattgattta
     2101 aaaggaaata agtaaatcaa caattaaata catctaaaag taagtttatt gcaatgaatt
     2161 gttaaagaaa ataattaata tggagtaaag ctattgcaat gaagtaacaa agaaaataat
     2221 taatatagag taagtttatt gtaatgaatt gttaaagaaa ataattaata tagggtaaat
     2281 ctatctcaat gaaataacaa agaaaagagt taatatagag tgaacttatt gcaatgaatc
     2341 aacaaaaaaa ataatcaata taaaaaggag aaatatttat gaatacagga tttccaaaag
     2401 actttttatg gggagcatca tcttctgcat ttcaagtgga aggagcttgg gataaagaca
     2461 ataaaggaaa aaccgtagcc gactataatt cttttaaaaa gtctcattta caggcagata
     2521 ctaaagttgc atcagacttt taccataatt atgaagaaga tattgaatta atgaaagaat
     2581 tagggatgaa aacatatcgt ttttcaattt catgggcaag gataatacca gatggtgaag
     2641 gagagataaa ccagaaaggt ttagactttt ataataagat aatagacaaa ctaatagagt
     2701 gtgatattga gccattcgta actttatacc attttgacct tccatttaaa ttagtagaga
     2761 aatataatgg ttgggaaagt agagaaactg tctatgcttt tgaaagattt gctaaaatat
     2821 gctttaagca ttttggagat agggtaaagt attggcaacc tcataatgaa cagaatttaa
     2881 ttgttagagt ggaggagaga attaatatat atgatgaaac agattcatgg aagatagata
     2941 aaatgcgtgc tcagatggat tataatcttt gcttagcaca tgcattagca gtcaatgcat
     3001 gtcatgagat gataaaagaa agtaaaattg gagcagcagt atcatcatct gtaacatatc
     3061 ctcttacttc taagccagaa gatgtatatg cagctagaat gaatgataat tttaaagtgt
     3121 actatatgct tgatatgcat cattatggag aatatccagg gtattatatg aagtatcttg
     3181 aaaaaagaaa tacagttcct cacatggaag atggggataa agagatactt aaaaaagcta
     3241 aaatggattt tatagctgta aattattata ggacaaattg tgcagaagct ttacctgaag
     3301 attcacaaca tccatttgga ttaagagaag gaactgttga ttttagtatg tatggactat
     3361 ttaaaatgtc aatgaatcct aacttagagg cttctgagta tggagcagca atagacccat
     3421 caggtcttag agttgcctta aatgaatatt ggcaaagata tcacttacct gttataataa
     3481 cagaaaatgg tctaggagct aaagatatat tagaagatgg aaaaattcat gatgactatc
     3541 gtatagacta tttaagaagt catataaatg catgtaaact agctattgaa gatggtgtag
     3601 agatgattgg atattgtcca tggtcattta cagacctttt aagttcttct caaggtttta
     3661 ataagcgtta tggacttgtt tatataaata gaactgacca tgaagtttta gatttaaaaa
     3721 gaatcaagaa agatagtttt tactggtata aagaagttat agagaacaat ggaatagtaa
     3781 aataaacata aatatatatt gagggtttta gtatgtacat tacaaaggta ttaaataatt
     3841 cgttgctact tgcaaaggat gataatgatg aagaaataat attgatgggg aaaggcattg
     3901 gccataatta taaggccggg tctgagttaa ataaaaaaga tatagaaaag atttttgttc
     3961 ttcatgatga aaatataaaa aagagtatta tacaattggc aacagaaata gatgcagaat
     4021 attttggtat agctcaaatg ataattgcat atggaataga aaaatataac ttaaaactta
     4081 tgaaccatat atatttggcc ttaactgacc acattgcctt tgcagtgaga cgctttaaag
     4141 aaggtataat gattgaaaat cactatttgt tcgaaatcaa ggaatttaat ccaaaagagt
     4201 atgatatagg aaaatatgct attactgttt ttaaggaggt tcttggttta gacctaccag
     4261 aagaagagat tggtaatata gcaggccatt ttataaatgc acaacaagat aatccttata
     4321 gtgatagaaa taagagaagt gctaaaattg taaatgctat tttgcaaata gttcattatc
     4381 acttttctat tgtatataat aaagagtcat tttattatag gagatttgta atgcatctaa
     4441 aggccttttc acaaagattt ctggctaatg aaccatcaag agaaaaaata gattttatat
     4501 atgagcaagt tcaaaaaaat tgtaaggaag agtatgaatg tgtgaaaaag gtaggagctt
     4561 atatgtataa ggaatactcc agagaattac caagacaaga ggagttatat ttgatgattc
     4621 atattcataa gattttagga gaattatgcg aaaatagtta gaatatctta atatgctagt
     4681 aatattataa aaaattaaaa attaactaca ctttaaatat gaacaattgc attacttaag
     4741 ggggttgtct taaaattgat ttttttaatt aaatttgaag ctcaactcct tttttaaacc
     4801 tttatatcat tacaacttta atgtttatac gcctgtactt tagatgtata tctacttttt
     4861 acatttctca acatgcaaac ctaccatttt ttgcagtatg ttaattgaaa taaaccattg
     4921 agttaaccct aaatttattc tggacttata ggaatagttc caaagatact ttaaaatatt
     4981 ttctggaata attgcttatt tcacatatgt tttattgtga tgttttgatt taataaattc
     5041 ttcaactctg tatatagtaa atgtattatc atgtttgttt tattcaagca cacttataat
     5101 acgcttgatt ttttcacaat gatgaaatat actaatgggt gttataggta taagaatcga
     5161 attacacttg aagaagtaag tgataaatga gatataaatc ctcatatgat aaactactat
     5221 ttcattgagg aacatattct aagtgatgtc aagatactac aatatgacta aattcctagt
     5281 gataaaaaag tctacgaatg agtgtagaaa ggggaactaa gatgaataaa gttttaatta
     5341 tagatgatga taaagatctt tgtattttgt taaaaaagag tatttcaatt gaacagattg
     5401 aagcaaacta ttgccattct gggagagatg gactggttga actttcaaaa caagattatc
     5461 agttagtagt tctagacgtg atgatgccag gtatggatgg ttttgaaact atggaacaaa
     5521 tacgaaaata tagcagtatt cccatcttaa tgctgacatc aaaaagcgat aattacagca
     5581 aagtgcatgg attgcgagct ggtgcagatg actatctaac caaaccattt gaaattacag
     5641 aatttactgc tcgtgtactg tctttaatcc gacgttacac ccgttttaat ccaaaaaatg
     5701 aggaaccaca gcaactttct ttcaaaggtt tgttgattga ccttgaccag catacagtga
     5761 ctaccaatgg cgaacctatg ttgcttccac ccaaggaatt tggtgtgctt ctttactgtg
     5821 caaggcatca gggtaagatt ttgacaaaac agcagattta tgaagctgtg tggggtgagc
     5881 catatgtata tgatgacagc aatatcatgg ctattatcag tcgcctaaga aaaaaaattg
     5941 aactagaccc atcatctccc atctatttgc aaacaataaa aggaattggg tatcaattca
     6001 acacggaggt atgaaaatgg atgaacaaac gataattctt ttcttattct tgctgtcgat
     6061 tggtctactt tttctttcca tatttgcttt ttccaagctt gggcatattc acaaacgcct
     6121 aaaagatata gaggagatac tggctgatgt acaaattgga aacgaaaatc gcaaaatact
     6181 aattaagcca tgtgatgtta tggctccatt ggtttatcag cttaatgaga ttgtctacga
     6241 ttatgaaaat aagttacttt ctctgaaaaa gtcggataaa gctagcaaac aacttatgac
     6301 aagtctatct catgatgttc gtacaccatt aacaactctg attggttatc tggatgctgt
     6361 acacagtggt atagttatcg gacaagaacg ggaggaatac cttgaaatcg ctaggcgtag
     6421 agcctacgac ctcaaggatt acattgatgt tctttttgac tggtttcggt tgaactctga
     6481 tgaatttacg ctgtcaatag aatctgtgga aattgcagag ttgtcacgca atattttgaa
     6541 agactggatt cctatattcc atgaaaagaa actgaatttt gaaattgata ttccagaaaa
     6601 tcgactaatg gtaaatctcg acccagatgg atattcccgt gtggttaata atctggttca
     6661 aaatgttctt gctcacagta aggctcggca aatcaagatt accatgagtg aggattccag
     6721 aatggtatta ctccgtgttg atgatgatgg tgttgggatt gccagagaaa atttaccaca
     6781 tatttttgag cgactgtata agtgtgataa aggacgttca gaaaaaggaa gtggtcttgg
     6841 tctatctatt gtcagtcaaa tggtggagcg aatgggtggt cagatttccg tagaaagtga
     6901 gattggaaag tataccgtat ttacagtgag cttaccatta atataaaatt gtttttcccc
     6961 catgtgacat gtatacacag ggggattttt tgtaagttat aaagttgcaa ggttaataca
     7021 aggttgttgc aaggttaatg taagattgag gtggtatgat agctttaata aacaaaggag
     7081 gattttatta tttatgaata atttaattat tgaaacaaaa aatctcacta agcaatatgg
     7141 tgctcaaaag agcgtttcta atttgaatat tcacgttcag aaaggtcgca tttacggatt
     7201 gctaggaaga aacggtgcgg gtaagacgac aactatgaaa atgttattgg gtctaacaaa
     7261 accaactact ggagaaatta aaatatttgg tcaggacatt cacaataacg agaagaagct
     7321 tcttcctcga attggatgtt tgattgaatc accagggttt tatcctaatc tgacaggaac
     7381 agaaaatctc aaaatctttg caaggcttag aggaatacca aaaaaaaatg cagtagaatc
     7441 agcactggat gtagtcggtt tgccaaacaa tgacaaaaag ttattttctc aatactctct
     7501 tggtatgaaa cagaggttgg cgattgctct tgccattatg catgacccac aactgttgat
     7561 tttggacgag cctatcaatg gacttgatcc tattggtatt gcagaagtaa gaaagtttat
     7621 tcgtgatctc tgcacagaac gaggtaaaac tattttgatt tccagtcaca ttctttctga
     7681 aattgccttg ttggcagatg acattggtat tattgaccat ggtattttgt tggaggaaga
     7741 aagtcttgcc caattagaag aaaaaaacag taaatacata cattttgctg tatctgatgc
     7801 agcacatgca gcaaggcttc tggaaaccca acttaacctg aaaaatttca aagtagcaga
     7861 taattacaat attatgctat atgatacaga tgtttctgtt gcagaaataa atcgtgcttt
     7921 cattctaaac aacattgagg tatcagaatc tcatttatgc gaagatactc ttgaagatta
     7981 cttcaagaaa gtaacagggg gtgaaggaat tgcttgatat aatttcatgt gaattttcaa
     8041 aactcaagcg gttaaaattt atactgattt ctattttagg tgcttgctta tttccaattc
     8101 ccgcaacaat tttgattgca aaggacaact tgccctttga gcaattgttc aaattagttg
     8161 tcaattttgg atattttttg ctattaccaa ttgtattaag tattgttgct agccaactat
     8221 tttttataga aagagataac gatgtattaa aaaatcttgc aacagtgcca gtgccaaaag
     8281 gaaaactggc tctggcaaaa ttggctgtgt tgctgtttat atcattgttc tactccgttg
     8341 cagggttagg agctactatc attggaggtt tcattgttgg aatagtagag ggggttactg
     8401 taaaattagg aatgagcatt gcttttggaa ttatgttttt tgttactgta cttcctgttg
     8461 tggtactcat tgtatatttc aatcgcagct atattttttc tattatactt tcttttgtat
     8521 atgctatttt caattttagt atatccttaa atattatcaa ttttgaacca aataacccac
     8581 tattaagcgt attacctgct cctgttataa tgcgttggtg gatggcattt tggggagatc
     8641 ccactggcga atacacggca cttagacagc catatttgct ctcaactcct gcttgtgtag
     8701 gtattttatg tttgataacc attatagcag tcctgctgat ttgtatgata tacaaaaagc
     8761 aggagaatta ggaggtatct atgcttgata taattactac agaatttcaa aaaataaaaa
     8821 gatataatat tctatggatt ggtgttgttg ctgttctgtt ttcagcacta ttggcagttt
     8881 ttcaggttgt aagttcacac ggcagtgacc ctctaacgta tgagagcttt gcaaacggag
     8941 ttatctggaa taatttcagt cttagttttc catttgggat tacacttatt ggaggatatc
     9001 ttattaaccg tgaatataca gaccaaacct taaaaaacat cctaactgtt cctatttcat
     9061 tacgcaaatt gctagtggga aaattaattg cggttggagg aattgctgta ttgttaagtc
     9121 ttttcagctt cttatgcaca tttattttgg ggttgttatt ttgcaatatc aatatatctg
     9181 ttacactcat tgtaaaatcc ttagttcaga ttttgtctgt taatctgtat tgttatgttg
     9241 ctgttttgcc gattattgct tattttggtc gtaaacaaaa tgcatttctg acgggtgtag
     9301 ggattgcatt tgtatacggg ttttgtggtg tgtttgttgc tggaagaaat ctgacagact
     9361 tttatccaat cacagcaggt cttggtctag ttggatacaa caatggtgct ggtactgttt
     9421 atcagccact tatcggtgct atgactttga ttgtaattct aatcttgaca actattctac
     9481 ttgtatttac acctaattat gataaagtta tgaccatatc taagaaaaaa gggataaagc
     9541 ataaaaagta ttctcataaa taacataaat attgtttcta ttatttaagt tgctagacag
     9601 tgaaaagaaa aaatctgtcg tacagtggct atatgctcat gtattcaaga agctacagtt
     9661 ttgatttttc ataactgtag cttctttttg tatttattaa atcacggcta tcataatact
     9721 tgcaataggt atagcgacaa gagagagtac tgtcatgtgt gttttatata ttacattaag
     9781 tatattctaa aatatatgtt ttaaataata tatttgaaaa taggaatatt taaataaaaa
     9841 aaataatatt atatatagtt gacaaaaatt ggcaaatgat ataatatata aatatagaaa
     9901 tagtaatcat tactattatt aaaaagggag gaattaatta tgaacttaaa aggaactaaa
     9961 acagaaaaga atttaatggc aggatttgca ggagagtcag aagcaagaaa taaatataca
    10021 tactacgctt caaaagcaag aaaagaaggt tataatcaaa tagctgctat atttgaagaa
    10081 actgctaaca atgaaaagga acatgctaaa ttatggttta aacttttaca tgatggtatg
    10141 ccttcaacag aggaaaattt aaaggatgca gcagcaggtg aaaactatga atggactgat
    10201 atgtatgcaa aatttgctaa agaagctaga gaagaaggat ttgacaagat agcttattta
    10261 tttgaagctg ttggaaaaat agagaaagag catgaagaaa gatacttaaa gcttttagaa
    10321 aacttaaatg aaggaaaaat atttaaaaga gatgaagaag ttgtttggca atgtcaaaac
    10381 tgtggtcatg tgtatgtagg tactgaagca ccagaaaaat gcccagtatg tgaccatcca
    10441 aaagcgtatt tcaatataaa agctgaaaat tattaaaaat tatcattatt aaaaaggaat
    10501 gttaactatg aaattttcta aacaacgaga actgatttta aatgaaatat taaataatcc
    10561 ggttcatcct actgcggatt acctatatga aaacttaaaa aaagataatc caaatttaag
    10621 cttaggaact gtttatagaa atttagctca gctaacggaa catggcttta taagaaaggt
    10681 tagtattcca gggtatccag atagatttga tggtcgaata gataatcact atcatatcat
    10741 atgtgaagta tgtggagagg tatacgattt agaatccgaa gttcttaata atttacaaga
    10801 attaatatct gaggagacag acataaaaat aacatcttat aacataagtt ttaaagggat
    10861 ttgtaataat tgtaaaaggt gtagccaagt aggttagttt tcaaattcta aaaataatta
    10921 atgaggtgag tttatgtgta gtgaacaaaa attttttata tgtgagactt gtggaaacct
    10981 tgtaggaatg atacaaagtg gtggtgtacc tattttctgt tgtggaaaac ctatgaagga
    11041 acttgttcct aatacaacag atgcagcagt tgaaaaacat gtacctgtaa ttgaagtgga
    11101 tggaaataat gttactgtta aagtaagtag cacaactcat ccaatgacta aagaacatca
    11161 tatagcatgg gtgtatctta tgactgaaca aggtagtcaa cgcaaatgtc tagcagtaga
    11221 tggagaacct gtagttaaat ttgctttgaa tgatgatgat aaagtgattt ctgcctatgc
    11281 ttactgtaat ttacatggat tatggaaggc tgaactgtaa tatttaaata aattgagtca
    11341 aagaggaggc tataaatgta ttttcataca aatagcctcc tataaattta tataaattca
    11401 agttttaatt ttgtttaatg tcaatataat ataaggagaa aataatatgt caatttataa
    11461 atgtagtgtt tgtggatata tttatgatga atctaagaat gataaaacat gggatgaatt
    11521 aagtgaagat tgggaatgcc ctgtatgtac aaaaggtcgt agttattttg gaaagataag
    11581 cactgtttac tatgaagaag atgaaaagat agcagaagac atagttgaaa atgaatctaa
    11641 attaaatact gaaaaagagg gagatttaaa ttacttaagt acatatttaa gaagagatga
    11701 tgaagttgag aaacatatgg atataattca cgaaatggct gttacaggaa aatctataat
    11761 agaacctatg agaactaaac taccagtaat ttcttgggat gatattttaa ttatgggtgc
    11821 tcaattaaat ccattacctt taaatgagca tgatgaagta aatacaacaa caataatagg
    11881 aaaaaaagca aagaaaccta tgattataga aaatccagtt tatatttccc acatgtcatt
    11941 tggagctttg tctaaagaac taaaaatagc tttagcaaag ggtgcagccc aaaataagac
    12001 agctatgtgt agtggtgaag gaggtatatt accagaagag aaggaagctt catacaagta
    12061 tatttttgag tatgtaccaa ataaatatag tgtaactgaa gaaaatctta aaaattctga
    12121 tgctatagag ataaaaattg gtcaaggaac aaaacctggt atgggaggtc atcttcctgg
    12181 cgaaaaagta actgaagaaa tagcaaaagt aagaaataag cctgtaggtc aagatgtaat
    12241 aagtccatct tgttttgagg aaatacaatc caaagaggat ttaaagaaac ttatagatga
    12301 acttagagaa gtttcagaag ggcgtccaat aggtgttaaa atttcagcag gtcatattga
    12361 aaaagatatg gaatttatag cttatgctaa acctgatttt gtaactattg atggtcgtgg
    12421 tggagctact ggagcaagtc caaagttgct taaagatgca acttcaattc caacaatatt
    12481 tgctctttat cgtgctagaa aatacataga tacacatgga ttagatatag atttggttat
    12541 aacaggtggg cttcgtatat ctactgattt tgctaaggca attgctatgg gggctgatgc
    12601 agttgctatt gcaagttctg ctcttatggc agcggcgtgt caacaatatc gtatatgtgg
    12661 ttctggtaag tgtcctgtag gagtagctac tcaggatgag gaattgagaa aaagattaca
    12721 tatagaaaat agtgctaata gagtggcaaa ctttttaaat gtaagtttag aagaacttaa
    12781 gacttttgct agaatttctg gtcataaaga tatacatgat ttaagtgttg atgatttata
    12841 tactgttaac tctgaaatat caaattatac aaacatacag catgtataga ttgaagtttg
    12901 aaactattag attttagata gctataaaag taaatgataa atatatttta taaaagagag
    12961 tgtcttaaaa tgaacttttt agttcataag tcactctttt ttgtatgaag ccaaatatat
    13021 ttatcatttt agcaatgaaa aagaatctta tctctttatt ttgagataga ctccttctat
    13081 ttatgaaaat taaagatatt aaattgtaaa atattctgtg atgtcgtact attaaactaa
    13141 agattctgta ctttaaagta aataaaaatt taaggagtgc ttattatgga aagattgaag
    13201 atacaagttt tagttgacaa taatacttat attgatagat actttgttgg agaaccagca
    13261 gtaagctatt atattgagat tgatggaaat aggattttgt ttgattcggg atattcagat
    13321 gtattcatat caaacgctga aaaattaaat atagatttag gaaatttaac tcatgttgtt
    13381 ttttctcatg gacataatga ccatacaaga ggtatccagt ttttagaaaa taggtatgat
    13441 ttatctacag ttgaacttat ttcacatcct aactgtttta tacctagaaa acatggagag
    13501 aaaagcatag gagctccatt ttctgctgag gaaattaaaa atatttttat gtataatcct
    13561 aaggataagc catttaatct aagtaagaat tgtgtgtttt taggtgaaat accttctata
    13621 aataattttg aaaaaagagc taaaattggt aaatgtaaaa taggagattt gtgggaagat
    13681 gattatgttt tagatgattc tgctatagtt tgtaaaactg ataaaggtat ttttattgtt
    13741 acaggatgtt ctcatagtgg aatttgtaat atagtagagt atgctaaaag ggtatgtgga
    13801 gatgatagag ttattggtat actaggtggt tttcatcttt ttgaattgaa taataggtta
    13861 gatagtacaa tacaatattt ggataaagaa gaaataagaa tgttgtaccc ttgtcactgt
    13921 gtttctttta aggtaaaagc taagatgaat gaaatattga atataaatga agttggagta
    13981 gggctgacca taagtatata gtatttgaaa gtatataaga tttaagttga aggttaaaaa
    14041 gatattttct atataaagta tcttttttgt tacgtttatt ttaagagaaa tcaatatact
    14101 tttatatgaa atattgtaaa aaaatttatt tttaatgaga atatattt
//
LOCUS       DAGQWR010000105        14124 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.105, whole genome shotgun sequence.
ACCESSION   DAGQWR010000105 DAGQWR010000000
VERSION     DAGQWR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.105"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(49..462)
                     /locus_tag="MIW95_002770"
     CDS             complement(49..462)
                     /locus_tag="MIW95_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBY3152082.1"
                     /translation="MILVSACLIGINCKYSGDNNENEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKIKSEKDL"
     gene            complement(530..2908)
                     /locus_tag="MIW95_002771"
     CDS             complement(530..2908)
                     /locus_tag="MIW95_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBY3152083.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSEISAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRNLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            3225..4676
                     /locus_tag="MIW95_002772"
     CDS             3225..4676
                     /locus_tag="MIW95_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBY3152084.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(4880..7393)
                     /locus_tag="MIW95_002773"
     CDS             complement(4880..7393)
                     /locus_tag="MIW95_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBY3152085.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTSDEQLEVLKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTDLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNADKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLEDMAKKHEIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLNGK
                     RIALAKGIDYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYEAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKSDKESENGNIENERDKIRREYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRITSKIIYNDRKIPMYVIGKIANIDSEKEEKNRLLDKVQR
                     DSLTNIYNAAYIRKLTTQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLVGDKVLIDV
                     AKVLDESFAKDDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVITKRGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(7616..8437)
                     /locus_tag="MIW95_002774"
     CDS             complement(7616..8437)
                     /locus_tag="MIW95_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY3152086.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMIVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEMR"
     gene            complement(8956..9432)
                     /locus_tag="MIW95_002775"
     CDS             complement(8956..9432)
                     /locus_tag="MIW95_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBY3152087.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVGNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(9466..10044)
                     /locus_tag="MIW95_002776"
     CDS             complement(9466..10044)
                     /locus_tag="MIW95_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBY3152088.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(10148..12766)
                     /locus_tag="MIW95_002777"
     CDS             complement(10148..12766)
                     /locus_tag="MIW95_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBY3152089.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIETGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MNRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMASSISIDSSISIDSSISIDSSISMDLDTSSSLGIPKELMGSNTNNIDTLSNTSK
                     IDTLNKNHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFK
                     KKDLNIDCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGY
                     APMMVTEYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSK
                     VTCMLDNLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKK
                     LDEIGTVAGHYYKGVE"
     gene            complement(13258..13941)
                     /locus_tag="MIW95_002778"
     CDS             complement(13258..13941)
                     /locus_tag="MIW95_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBY3152090.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT         4451 a         2526 c         1525 g         5622 t
ORIGIN      
        1 tacatcaata ttagctttat atactttttt tactctttta tctattttct ataaatcttt
       61 ttcactctta atttttattc cttcttcttc taacagcctt gctgttatac ctttccctgg
      121 tatcttttta cctgaaaaac taccatcata tattagatta catccacaag aggggctacc
      181 ctcttttagt attgcttcgc tacatacata taaatttgct atttttagag tttcattagc
      241 ccctcttaca aagttttctg ttacatcctt attatcttta gtaattactt ttccatcaac
      301 tatctcagca ggtggtcttg gtgtagatag accacctaac tgctctgggc atacaagtgt
      361 aaattctttg ccttttaaat attccttgac tttttcattt tcattattat cgccactata
      421 tttacaattt attcctatta aacatgcaga taccaatatc ataaaaatgt gctcctatac
      481 aaattcttaa tatattctta tacaaattac taatgtattc ttatacaaat tattttaatt
      541 taacaattgt tactcctgct ccaccttctc catattgacc acctcgttgt gattttacat
      601 gtctatttct tcttaaaatg tcttgtagcc ctgcttttaa tactcctgtg cctattccat
      661 gtattactgt tacagactct aaacctgcca catatgcatc atcaagatac ttttctactt
      721 ccattatagc ttcttctagg tttaaccctc taaggtcaac ttcatttttt acactaccag
      781 attttgacct tataatgttt ctcgttgatt tagttacatt tgttgaaaca tctttgcgtg
      841 ttttttgtaa agatttaaaa ggtagagtca tcttcattat accaatttgc actactgctt
      901 cttttctctt tttatctact gaaactacac taccattttg atttaaagtt attaccttaa
      961 cttcttcacc tggctttaag tcttttatct ccttatttga tacctttgga actatcatac
     1021 tttttactgt tggctgaaga cttcccatag agctggtcaa ttcttttcta agttcttcta
     1081 tcttacgatt cttttctttt gaagctcttt cttgttcaag atttctaagt tcttttatta
     1141 taatatctac ttcttcttta gcttgtctta taattgaaaa tgcttctgat ttagcttttt
     1201 caatcatctt atctctttga ctgaccaatt tttcaagttt ttcgtcatac tctactttca
     1261 gtttttcaat ctcttctttt aatctttcag cttcttctct atcttctact gctttgattc
     1321 tattcttttc aacattctgt aacacatctt ctaaagctat gttttctgta tttatatatt
     1381 ctttagcacg tgaaatcaca taatcactta gcccaagttt tctagaaatc tcaaacgcat
     1441 ttgattttcc gggaacacct ataagtaact tatatgttgg gcttaaagtt tctatatcaa
     1501 attcaactgc tgcattttct acacctggtt tagtaagagc ataattttta agctcactat
     1561 agtgagtggt agctatacat ttagctccta cagaatttat atcttctaat actgctattg
     1621 caagggcagc tccttcaaca gggtctgttc ctgctcctaa ctcgtcaaat ataactaaag
     1681 agtctgctgt tacgttttgt aatatagata ctatattcgt catatgagat gaaaatgttg
     1741 atagactctg ttcaatactc tgttcatctc ctatatctgc aaatacatta tcatatacac
     1801 acatactact tccataatca gcaggtatat gaagtccact ttgtgtcata agtgcaaata
     1861 gacctacagt ttttattgta acagtcttac cacctgtgtt tggacctgtt atcaccaatg
     1921 tatggaagtc tcttcctaaa taaattgtat ttgctactac cttcttttta tccagcagtg
     1981 gatgtcttcc attttttatg tttaaatatt tatcctcatt taaagttggt tctattcctc
     2041 tcatctgtat agaaagtttt ccctttgaaa atgcaaagtc caatcttcca agtatctctt
     2101 gattagagat taaatcttcg ctcacttctc caaccatagc tgaaatctct gataaaattc
     2161 tttctatttc ctcttgctct cctagtttca actgcctcaa ttcattgttc atctctacta
     2221 tagtcattgg ctctataaaa agtgtcgcac cagaagatga ctggtcatgc actataccag
     2281 ctacttgcga cctatattca gattttacag gtactacaaa tctatcacct cttagagaaa
     2341 ttattgcatc ttgtaagtac ttttgataag tagttgaact tattattgaa tttaattttg
     2401 acctaatgga ttgatttttt tgggctattc ttcttctgat atctctaagt gtacttgaag
     2461 cattgtcaga tatttctact tcactaatta tagcattgta tatctggtct tctatgtcct
     2521 tgtacacata tagagaattt gatagagatt gtattattgg ataattaaaa tcttcttcat
     2581 cactactaga taagctattt cttaatcttc tagccactct taaggtatct gctatcataa
     2641 tcagacttcc tgggtcaaga gaagcaccta tataagctct ctttacctta tcttctatat
     2701 catgaatacc ttccaaccct actgaacctc tctttataat aattgattga gcttcactag
     2761 tttcttctag catagatttc acttctacaa aatcagtatt tggtactaaa ttttctatat
     2821 attttagacc taaagaagat gaggctttct tctttaacaa atctattatt ttattgtatt
     2881 ctagaactct taacgatttc tcattcattt tctatattca ccatcctcga tattttaact
     2941 tttttattta attttaaaaa tgtatgtttt caagcatttc caataaaaaa tgcaagtttt
     3001 atttcttttt catttctaaa attattatat tttttctctt ttagatatat tttacacata
     3061 ttttgtatta tactatgtat gtagctatct atatattata cataaacatg ctttatttgt
     3121 aatataatct gttaaaaaat cttctttaat atttttaaag gagtttattt tagcttataa
     3181 aaaaatagct acttatatag ataaattaag gaggaacatg agtcatggga aacgtattag
     3241 aaggattaaa accagaaagt gtttttaaaa actttgagaa aatatctcaa attcctagag
     3301 gttctggaaa tgaaaaaggc ataagtgatt ttttacttag ctttggtaaa aatttaggtc
     3361 ttgaaactat tcaggatgaa agcttaaaca taataataag aaagcctgct acaaaaggat
     3421 atgaaaattg tccaggtgta gttcttcaag gacatatgga tatggtttgc gaaaaagaaa
     3481 agaatgttga acatgatttt cttaaagacc ctataaaatt aagaattgat ggtgacatga
     3541 tatatgctac tggaactact ctaggagctg acaatggtat agctgttgct atgggtatgg
     3601 ctatattaga agataatact ttagagcatc ctgcactaga agttttagtt actgttaatg
     3661 aagaagatgg tatgaatggt gcagatgcac tagaccctag cttaattaaa ggtcaatata
     3721 tcctaaatat ggactccgag gaggaaggat acttattagt tagctgtgct ggtggtaaaa
     3781 cttgtgttgt tagtttacct gttgaataca aggaagttaa aggtgacaaa caaggattat
     3841 tagttgaggt tactggacta ttaggtggac actctggtat ggaaattgtt ttacaaagag
     3901 caaatgctaa caaagcaata gctagagtat taagtgtatt gaatgttgat tatgaattag
     3961 cttctgttga tggtggtact aagcacaatg ctatacctcg tgaagctaaa tgtgtaatag
     4021 ctgttaataa agctgatgtt gaatctgcta aaaaacaaat aaatgacatt ttaactgcat
     4081 ttaaacatga gtttactact tctgaccctg gcatgactta ctcagtagct gaaacttctg
     4141 tagacaaagt tcttactaaa gattgtaaag aaaaagttgt tcaaatgtct tgcttaactc
     4201 ctcatggagt tcaatcagta agtcttgata tagaaggatt agttgaaagt tctactaact
     4261 tcgctataat tgaaactaaa gaatctacta ttgaattttt aacttctgtt agaagttctg
     4321 ttatgtctat tagagatgaa atcgctgata gaataagact tttggctcaa gctttaggtg
     4381 ctaattacga ccttatagct caatacccag cttgggaatt taaaaaaggc tcaaaattag
     4441 aaaaaatatg cagtgaaact tatgagaaat taactggtaa agttcctact gttatggctc
     4501 tacatgctgg tcttgagtgt ggattattac ttgataaact tcctcatgct gaagctatct
     4561 ctataggacc agatatgttt gatgttcata ctccaaatga acatgttagt atcccttctg
     4621 tagctaatgt ttgggattat gttattgaaa tattaaaatc tatgaaccaa tattaaattt
     4681 aagttttgat tctaaataga ttttaatttt aagtaatcta tataaaatta actctgtaaa
     4741 ttaatactac tgttagtata atatttactt tcaatattaa cttatagaaa taaagcctaa
     4801 tgatagataa taacatgtat cattaggctt tattgtaatt taaatattat taaactttat
     4861 tctgttttaa aattaattct tatactattt taaatctatt tcttccttgt ctcttagaat
     4921 cataaagagc attatctgct atttgataca gttcatcata ctcttgacca cgctttgtta
     4981 taactacccc catactaata gataaacttt ctccatttgg caacttaatg agatgaatat
     5041 gattgtatac atcattacat ttattgataa gttcttcctt atttattata tcatttatat
     5101 aaacactaaa ttcatctcca cctaaacgac ctactatatc atcttttgca aaactttcat
     5161 caagtacctt tgcaacatca atcagtactt tatctcctac caggtgacca taagtatcat
     5221 taattgactt aaaataatca atatctataa tcaataaagc tccatttttt tcagttgata
     5281 aatttaataa attttgtgta gttaattttc taatatatgc tgcattataa atatttgtta
     5341 aactatccct ttgaacttta tccaacaatc tattcttttc ctctttttca gaatcaatat
     5401 tagctatttt accaattaca tacattggta tttttctatc attataaatt atttttgaag
     5461 taattctaat ccaatgaaaa gtatcatcaa gcatgataga atacatttca gtagtcccat
     5521 tttcttgtga ttttattaca tcaaaaaact ttttattata ttctcttctt attttatctc
     5581 tttcattttc tatattgcca ttttcacttt ccttatcact tttatccaaa ccataataag
     5641 tctcaatacc cttttctggc atagaaatta tcaatttatc ttgcttataa tcatactcaa
     5701 aaaagtattc acttgtcatt tcatacaact gagtatgttt tttgagttcc aaagatatct
     5761 tttcgttcat acttgtacga gtatgtaaac caaatgccat aaatccaata atacaaatta
     5821 ataaacttgc tacaataaaa atagtctcaa ttggattctg ttgcataata taaagcaacg
     5881 agaaatcttg tctatatgta gtattttggt agacaattga ttgcattttt tctgatgaaa
     5941 tacttataat agctttatta aatatattca ataattcttg tttatctttt ttaattatgc
     6001 caaaacaaat actctttgct tcataagttt gaggaactag gcgcacattt ttatacttag
     6061 gttgattcat atagtactgc atagaataac cttctccata agtgtaatct gcatagcctt
     6121 tattaacagc ttctatacac tcttctaaat tatcatacca aactatattt tcttcaaact
     6181 gaccataata atcaatacct ttggctagag ctatacgctt tccatttaga ctattctcat
     6241 ttaatttatt attaatcatc atagtatatt gagtagaaag aaaaggccga gacatagcta
     6301 tattatgttc acgagagtac tcataatcat aaatcattcc tgctacaata tcaatttcat
     6361 gttttttagc catatcttcc aaatcttctt gagattttac aggcacaagt tcaaattcca
     6421 aacctgtttt atcacatata tgatttaaca aatcaattga tatacctctt aattctcctg
     6481 tatctttatc tgcattttga aacggtggtt tgtttaaaag tactcctact tttaattttt
     6541 ctgtatcttt aatgtaatta gtttcttcat cagataaaaa catttgatta ttctctggcg
     6601 taaagtattt ctcatataaa gtagttgaaa aatatgggtc cttctgttca atattattaa
     6661 ttgctgaatt aagcttttga gttaagtctg tactgccttt tggagtaatg aaataaaatg
     6721 gctttgatga gaattttgct acagtacgca caccttctat tgggttcata ctaacattaa
     6781 gaagtacatc tgctttacca ctctttagta cttctaattg ctcgtcactt gttttacaat
     6841 ataccaattc aggtgacata agattcattt tacaatactc atctaattct ttaagcctag
     6901 tatcagaatt ttctattaca gcaatacgaa tttttttcac atcttgcgaa ctcattatgt
     6961 tatcaatatc tgtattttca tataatgctt gtagtactgt atttgctact ccataactgt
     7021 ttcccgcata attatatatt ttttgcattg gctcattgta taacatacct cccattatat
     7081 caatttcacc attttctaac atcttcatta attttgtaat agattcattt atctctccag
     7141 gaacttgaac aaattcataa ttccatccag tatactgtgc aatttctagc aaatattcat
     7201 atgtataccc aaaatagtta ccttgctcat ctatttcagt taaacctttt tgaataggat
     7261 aagcaacacg aatggtacga ttatcattac ctatagcatt gataccaaaa gaaaatatgc
     7321 aagaagtgaa tatagatgta attacaataa ttaataaaag taattttttt aaattcacta
     7381 cacaatcctc catatagtta agatttacag tataaattac tgcataaact tgtagacatg
     7441 aaaaaataga ttagtatata aaaggatata taaatctact tagaaattta aatatcctta
     7501 atatatacaa tatatttaaa ttataactca tttatatatc tattacaaca aatttaccct
     7561 gctaaatacc tactctatat cccataattt tacaagttgt aatgaagttt aagttctatc
     7621 tcatttcaaa tttagcagtg tttctattta cagttctttt atatttcaca tctggataac
     7681 ccaatataaa tgaagtgata actgcttcgt tctcgctcat tcctaacagt tcttttatct
     7741 ttggctctac atttgatgcc ataacaaaaa atccattata acaaattcct aatccttgag
     7801 aataggccat aagttccata ttagatgcag ccaatcctcc atctacataa gcacttccac
     7861 ctagagacat atcacctaca actatcataa gtagtggggc atcataaaat aaagaatcat
     7921 ttccgttctc tttatatctt ttgtacattt tcttaaatgt atttttatag atagaacgta
     7981 cagggtcatt gtcatcagta tctaatgcca aattataaag accttgtatt gccagttctt
     8041 tcacttcttt tagcttttct tttactaata tgtatcttat aggctgtcta ttaccacctg
     8101 ttggtgtgta tctaccagct tccactatgt tttttatttt ttcttcttct acctctatat
     8161 ctttatattg tcttatactt cttctaaatt taataaaatt caataacttt tcgggttcta
     8221 attcaaaact ttcacgattg tattctatta cttcattttt atcaaatcct tgcatttcta
     8281 ttgcatctgt tgggcaaatt gccatacagt gaccacagtt aatacagaga atatttttaa
     8341 cctttgcttt atcatcctta acttttattg catttacaag acagtctgat tcacacatcc
     8401 cacagcctac acatttttct aaatttatgt ctatcattcc aaattattcc tccttttaaa
     8461 aaatttactt tagtataaaa caaatagttg tctaagtcaa tttttatttt tttaacaata
     8521 tactagcctt aactacactt taatggagaa aaatatacat aaaattagaa cttaaattag
     8581 aatttattca aaataaaaga taaaagaagt aaatgtctaa aagtgaactt tttagttaat
     8641 gagacattct ttattgtatg aaatcaagtg gatatttatc atttcaacaa taaaaaaggc
     8701 ttacctctat tttggatatg tccttgttaa gtagacagac taaaaaaggc tatctttatg
     8761 cactgagata gccccttttc ccaactacta ttgtttattt taatattata tcaataaatt
     8821 taatttgcta taacctgtaa gttacatttt aatttgatgt aactttaaat tacattttta
     8881 agtaatatct ttaaattata ttcttaaaat atatcattaa actacatttt aattaatcta
     8941 aaaactatat cttaattaat ctaagaaact tacattataa tcttttagtt tcccattatc
     9001 atatacaatc tttgcactaa agatatttcc agtcttagcc caatttaaga acaacctgtc
     9061 accttcccac atatttaaat cgaatatttt atcattacca acccaaatta aatttccttc
     9121 tttacaatca tttaaatctt tttcagacaa ttcaccttca aactcatctg agaaatacaa
     9181 aaacatatac caatcttctt catctttaaa ttttggaaaa gttatcaacc ctttatatga
     9241 caatgattta agagtaagac cagtctcttc ctttacttcc ctaattacac attcttctgg
     9301 agtctctcct tgctcaaact ttcctccaac tcctacatac ttaccttcat gtatatcatc
     9361 tttcttttta tttctgtaca acatcaaagt cttgtcatct ttttctatat aacaaatagt
     9421 tgtaagtttc atgtatctcg ccttactttc tctttatatt ctcaattaat tattttcagc
     9481 actttcaagc acatcactta tattcttcat tgcattaaga gaaagttctg caaattcatc
     9541 aaatggaatc cctatatctt ctatacttct cattgcatct ctatttacat ttgaagcaaa
     9601 tctcgtttct ttcattcttt taataacaga cttagtcttt acactagtaa gttttttatc
     9661 tggataaact aaagctatag cagttataaa cccagttata gggtctgctg catatattgc
     9721 tttttccatt aagctagttc ttgtaactcc cattccatca tgatgaccaa gtattgctct
     9781 atacatttct tcgtcaccga agttttcttt tttaagcagt tctactgttg ttgtagcatg
     9841 tccttcacat atttctcctg gctttcttcc aactatatct gaatccaaat catgtaaaag
     9901 acctgccact gcccactctt cttctttttc tggctctagt cttttggcta attctctcat
     9961 aacagcttca acagcataac aatgttttct cattctatca gtttctaaat acttaaataa
    10021 aatttctaaa gcttcttctc tcattttcac caatccccct gttttttcac atatatgata
    10081 tttttatata ttaatacaca taaattcaag tctttatata tattcatgta tatttttcat
    10141 gttatatcta ttctacacct ttatagtagt gtcctgcaac tgttccaatt tcatctaatt
    10201 tcttatacaa atctgtagcc ttttttccaa gtttataatt attactcaat acctcaatgt
    10261 gagcttcaat aaccttttta accatctcac tatcttcaac tgtaaaatct atcctaaata
    10321 catctattcc aatttcctct aactcatata gattgtccaa catacaagta accttcgaat
    10381 tgtaaattgt acttctacag aatatatctt gtgttatcct ataacttgca tccgtaaagt
    10441 ctcttaaagc ataactactt tccttacatt tagcaactct cttgtccttt ttacaatctc
    10501 taacaactac tcccatagga cagtattctg taaccatcat aggagcatat ccatatatta
    10561 ctgcttcaat ttctacatct gtatatttta aagtttgttt aatttcattt aagtttagtt
    10621 cttgtgacaa acaaactgta cttgctccct cttctttgaa gtggtttata ctttcactat
    10681 tgaatgcatt taagtagcaa tctatattta agtctttttt cttaaagtat tccatagagc
    10741 cccagttacc aatttgaacc ttattaattc cttttatatt atccaatatc tcaaattgct
    10801 tgtactcttt atttctaaca atccttggtg ctgaatagat taattttttg tcagtgactg
    10861 ctgtattata gtggttctta ttcaaagtat ctattttact tgtatttgac aaagtatcta
    10921 tgttatttgt atttgaaccc attagttctt taggaatacc taaagacgaa gaagtatcta
    10981 aatccataga aatacttgaa tctatagaaa tacttgaatc tatagaaata cttgaatcta
    11041 tagaaatact tgaggccatt tgtaaagctt ttgataatgt atttatatct tcataataga
    11101 ttaaatctac attataattt aatactgctt ctaattgctc caagttctta actttaactc
    11161 taatcttagg aactgtatca agttttttct gtttatttac tctatttggt tggtatttta
    11221 atctattctt cttaaatttt ctatctttta tctttattct ttcttcatct aaaagctcaa
    11281 tacaatccct tctcatttga tttaacacac ttataggcat acttacacct tcatccaggt
    11341 caatttctat ctcttttaac ttatatggtg tatttccaag cttttgaatt tgtgtttcta
    11401 ttttttcttt acttaaagcc acctttatag ctttttcaac tattttgtct ccttctacca
    11461 ccgatttatt tccacaaaaa tcacttataa aaagctctgg ctttttacct agtttaatac
    11521 tcacttttgc acgtatatta gtttttatga actctttatc ttgctgaaat gtagcttgta
    11581 ctctattcat taattcgctg tcagaagtct tatatataag ctgatttctc tttgcttctc
    11641 caacaaaatc taattctatc aaatcccctt tatgtgctat tgtagttatc tctccatcct
    11701 ttattattct tccaatagta cctccaccta agttaattcc atcacctttc ttaagcgtat
    11761 tttctagcat tactctaagt cttttgttct ttctattata atctaaaact tttccaatat
    11821 atagcccttg attattaggt ctattagagt tcattatatc cttaccaact tcacctaata
    11881 tatatccttt tgtaaacttt ctattaaata tggtatacaa attatttatt atttcctcag
    11941 aaatacttat ttttttctta tctacaaatt cattaatcgc ttctctatat cctgaaacca
    12001 cagtagctac atactctggt tttttcattc tcccttcaat ttttaaagat aatacacctg
    12061 tttcaattat tttatccaaa tcttctatag tacttaaatc tctaggactc aacaaataat
    12121 ctccattact ttttactact tctcctgtat ttatatctat caactcatac ttttgtctac
    12181 atggctgtgc acacctacct ctatttccag acctattgcc tatcatacta ctcataaggc
    12241 attgacccga gtaacataca cataaagctc catgtacaaa aacttctata tctacagttg
    12301 tattttcaca aatccatttt atctcatcaa catttaattc tcttgctaaa acaactctat
    12361 caaaccctac actctgaagg tattttacat cttctaagga atgtgctacc atttgagtgc
    12421 ttgcatgtag ttcaaagtca ggtaatagtt tttttataag tcttgccatt cctatatcct
    12481 gtactattaa agcatcaata tctatatcgt ataaaaattt tacatactct acaaaatctt
    12541 ctatttcata ctgttttatc aatgtattta cagtaacaaa gacttgcact ccccttatat
    12601 gagagtattt aacagcctca atcagctcat ttctatcaaa gttatttgca gatgctctag
    12661 cactaaagtc tttaccacct aaatacacag catttgctcc gttttgaact gctgctttta
    12721 atgaatcaaa agagcctact ggtgcaagta attctatttt tttcatattt ttctcctcta
    12781 tttatacttg ttaggttaat ataattatat ctataactta aatatttgta ttaaatttat
    12841 atatttctta ctatatttta aattcttcaa ttaatttata tattagttat ttatatttgg
    12901 tccatttgag ttaacatata cattatttac tatatttagt atatttctat taacttatat
    12961 attgattact gtattttaaa ttcttcaatt aatttatata ctgcttacta aatttaaaac
    13021 attttaatta atttgtacac tgtttactat acttgaaata cttctattaa tctatatatt
    13081 gcctactata cttaagacat tttaattaat ttatacactg cttactatac ttaaaatact
    13141 tctattaact tatatattgc ttactatatt ttaaattctt taattaatct atacaatatt
    13201 tgctatatat aaagtatatc aaataacagc ttcatttttt tgtaaattta cagaaagtta
    13261 attttcatta aatgaatcat ctttattgat aaacataatt ccaaagacca ctgccaaaat
    13321 aaaaaaacat attagtattg atttttccac gaaaattttt gtaaaaaaag ttcctaaaat
    13381 agctccagat ataaagaata taataatagc ataatactgt aaactctttt ttatcataaa
    13441 aatattctta gtatgtatat aatgaaataa agtttctgta gcacttctta aatttccagt
    13501 acacattgtc gttgcaaaac tattcccatt tacttttcta aaactctcta cttgcattga
    13561 acaaataaaa gaaatagcaa catttacaaa catattaaat cttcctctag gaataaatga
    13621 aacaataagt attgtaatca tttcaattat aatgataacc tgacgccaat gaatctttgt
    13681 cttattattt gtaaaatact ttcttatctt ctcggctata aatacaccta acataaatgc
    13741 caaaatagga atcaaataat aaaatgattc ctttacatta ccttctgcta tattaagccc
    13801 aaataaaact atatttccag tttgtgcatt agcaaagact ttacctctta ccacataagt
    13861 ataaatatcc aaaaatcctc ctgttattgc caacaaagaa cataaaaaga atgattcaga
    13921 catctgactc ttgcttgtca tatataaacc tctcgctttc taatgataga tacaaaatca
    13981 aaatcatttt agattctatt tttcattgat ataaaaaaat tatttatatt taaatattat
    14041 ctatatttat acatcattta tatttataca ttatttgtat ttatatatca ttcatattta
    14101 tttatattta aacattatct atat
//
LOCUS       DAGQWR010000106        14078 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.106, whole genome shotgun sequence.
ACCESSION   DAGQWR010000106 DAGQWR010000000
VERSION     DAGQWR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14078
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.106"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            202..1008
                     /locus_tag="MIW95_002779"
     CDS             202..1008
                     /locus_tag="MIW95_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY3152091.1"
                     /translation="MGMILSTLGINLYLAPAKLLSGGVAGICVILYKLFGINQGISSF
                     LMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLTQDIAKYFPIDDTMLQCIYGGAL
                     TGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNTFLFINFFVVCAGAFLFGAKLVM
                     YTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEISKVIMNKMGRGVTFLEAEGAYT
                     QNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNNVEEVKGGGFKDKFL"
     gene            1135..1407
                     /locus_tag="MIW95_002780"
     CDS             1135..1407
                     /locus_tag="MIW95_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152092.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(1570..2142)
                     /locus_tag="MIW95_002781"
     CDS             complement(1570..2142)
                     /locus_tag="MIW95_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY3152093.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            2326..3168
                     /locus_tag="MIW95_002782"
     CDS             2326..3168
                     /locus_tag="MIW95_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBY3152094.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            complement(3271..4347)
                     /locus_tag="MIW95_002783"
     CDS             complement(3271..4347)
                     /locus_tag="MIW95_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY3152095.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            4844..6247
                     /locus_tag="MIW95_002784"
     CDS             4844..6247
                     /locus_tag="MIW95_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY3152096.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFMLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     TSLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    6338..6578
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6621..7319
                     /locus_tag="MIW95_002785"
     CDS             6621..7319
                     /locus_tag="MIW95_002785"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBY3152097.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILKGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            7342..8343
                     /gene="trpS"
                     /locus_tag="MIW95_002786"
     CDS             7342..8343
                     /gene="trpS"
                     /locus_tag="MIW95_002786"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBY3152098.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANAIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(8995..11625)
                     /gene="cdtB"
                     /locus_tag="MIW95_002787"
     CDS             complement(8995..11625)
                     /gene="cdtB"
                     /locus_tag="MIW95_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094607992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin binding subunit
                     CdtB"
                     /protein_id="HBY3152099.1"
                     /translation="MKIQMRNKKVLSFLTLTAIVSQALVYPVYAQTSTSNHSNKKKEI
                     VNEDILPNNGLMGYYFTDEHFKDLKLMAPIKDGNLKFEEKKVDKLLDKDKSDVKSIRW
                     TGRIIPSKDGEYTLSTDRDDVLMQVNTESTISNTLKVNMKKGKEYKVRIELQDKNLGS
                     IDNLSSPNLYWELDGMKKIIPEENLFLRDYSNIEKDDPFIPNNNFFDPKLMSDWEDED
                     LDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDPYTDYEK
                     ASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTES
                     NTAGVSVNVGYQNGFTANVTTNYSHTTDNSTAVQDSNGESWNTGLSINKGESAYINAN
                     VRYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQENQIGNNLSPGDTYPKKGLSPLALN
                     TMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYIS
                     QIDSISASIILDTENESYERRVTAKNLQDPEDKTPELTIGEAIEKAFGATKKDGLLYF
                     NDIPIDESCVELIFDDNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDD
                     YNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLTSN
                     SIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITE
                     LNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYFAPTQTNKEAL
                     DYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYK
                     KLRIYAITPDDRELLVLSVD"
     gene            complement(11678..13069)
                     /gene="cdtA"
                     /locus_tag="MIW95_002788"
     CDS             complement(11678..13069)
                     /gene="cdtA"
                     /locus_tag="MIW95_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin enzymatic subunit
                     CdtA"
                     /protein_id="HBY3152100.1"
                     /translation="MKKFRKHKRISNCISILLILYLTLGGLLPNNIYAQDLQSYSEKV
                     CNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKY
                     SQTRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTEN
                     QNEISLEKFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLP
                     YTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKA
                     NYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKR
                     EPIPTNLTVYRRSGPQEFGLTLTSPEYDFNKLENIDAFKSKWEGQALSYPNFISTSIG
                     SVNMSAFAKRKIVLRITIPKGSPGAYLSAIPGYAGEYEVLLNHGSKFKINKIDSYKDG
                     TITKLIVDATLIP"
     gene            complement(13459..>14078)
                     /gene="cdtR"
                     /locus_tag="MIW95_002789"
     CDS             complement(13459..>14078)
                     /gene="cdtR"
                     /locus_tag="MIW95_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBY3152101.1"
                     /translation="LKELTEKNKVKIYFIVVDAKYKISNELCDGLWIAQKIRESDYIS
                     PIIFLTNHIEMILGIFDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYVKEKNYRSN
                     FFTIYSDSSLWKISFDEVIYFETSAIPHKIKLVTTSRIFEFYKSLRSLSDLDACFIRV
                     HKSFVVNKYHIVSLDLKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         4926 a         1834 c         2096 g         5222 t
ORIGIN      
        1 ataaatatat taaaagaagg attaagacta ataacaattc aaaataaata acttatgtaa
       61 gaaccgtagg aactacgggg atagcttgtg gagaattagt aagacatatt ttagtatgca
      121 aaattctatg aagcaggaac cctgtgactt tagtcatggg aggttcagaa taacatattt
      181 ttacaatatg gtctgattat attgggtatg attctatcaa ccttaggaat aaatttatat
      241 ctagcaccag ccaagttatt aagtggagga gtagctggaa tttgtgttat attatacaaa
      301 ttatttggta taaatcaggg aatatcaagt ttcttgatga acataccaat atttattata
      361 gctagaaagt attttgataa taaatttttg ctaataagtt ttgtcaatat gttactgttt
      421 tctctagcac tggggctaac acaagatatt gctaaatatt tcccaataga tgatactatg
      481 ctacagtgta tttatggtgg tgcattaact ggtataggta tggggttaac ttttaaagca
      541 agagctactg ctggaggact tgatataata gcagccataa tgaaaagaaa gtatgatata
      601 cctatgaaaa atacattttt atttattaat ttttttgtag tatgtgcagg agctttctta
      661 tttggagcta aattggtgat gtacacttta attacaatgt atataatttc ttttactatg
      721 gatataggaa aagattgctt tgatagaaaa aaatctatac ttttaatatc aaataaatat
      781 gatgaaatat ccaaagtgat aatgaataaa atgggtagag gagtcacttt tttggaggca
      841 gaaggtgcat atacacaaaa taagaaaaag atgatttatt gcataatatc tgcaaatgaa
      901 ataggtaaat ttaaagattt aatttatgat atggatagtg aagcttttat ttctgtcaat
      961 aacgttgaag aagtaaaagg tggaggtttt aaggataaat ttctttaaga ttatatatgt
     1021 taattatata tgttaaaaaa tgccatatgt attgagatta tacctagtta gatgtatact
     1081 gtagttaatg gggtacatga agtactcaat aatatactag gaggtataag aattatgtta
     1141 agagagagat ttgtagtaag aaatcatcat aatccacata caagtgggtg ttgtgggact
     1201 ggaggagaag gatgctgtgg aggagataac catgaacatt cacatcaaca ttctcacagt
     1261 catgggcatg aacatgagca tgtaaatggt gtaggtcacg accataatca tgatgatggt
     1321 ttttcaggta ctcatgagca tagccatgag catacacata cacatggtca tgaacattca
     1381 gatgggcata cacatgaaca taaataaaaa cattttaaat ttataagaga tttttatttt
     1441 gtgattccat gaaggtttaa ataaaaattg aatgttttaa gtaaaaatta ttgagaataa
     1501 ataagaaagt gaactgttaa agtagataac ttaaatagtt cacttttatt aattatctaa
     1561 gtattaagtt taaatccaat gattaactaa aaaagaagct atattttcat aaggtatttt
     1621 ttctgttttt aataaacttt ggacaactaa accatcaact attgacatga gtaaaaaaga
     1681 aaatgattca gcatcctgtt cattttcgct actattatat ttttctagtg attcaacaat
     1741 ttcagattta agagtagttc tccattcaat atattttaat cttattcttt cctttaaatc
     1801 attatttgaa gtaattgcct cacaaataag atacatatgt accctaccac gactttctat
     1861 agtagatatt ttctctaaga ttaagttaac catttctatt tgagaatttt ttgatttgac
     1921 attttttaca cagtttagta tagcactagt tataacttct aaatgagtat ccgcaatatc
     1981 atatataata tcatctttag atgcgtagtg ataatacaaa gtacctttac ttatatttac
     2041 atcttttgca atatcagcaa ggctagtatt ttgaactcca tttttggcta ttaaattggt
     2101 tgcagatgat aaaatagcct gtttcatatt atttgttttc ataaaaccta cctttccatg
     2161 tatatttata acaaattaat aacattataa gcttatgaat atttattgtc aataaagcga
     2221 gaatctatat ctattgactt tttttcattc aatatataaa ataataatta aatcggtcgt
     2281 acgaccgact gaataaataa aataatatct tatagtgagg ttaatatgga taaaataaaa
     2341 ttgatagtgg acagtgcatg tgacttacct gatgatataa tagaaaaata caacatagaa
     2401 gttgttggtt taaatgtatc ttttggagaa gaaagttata tatctggaaa ggaaatagat
     2461 aatgaaactt tctatagaaa aatgagtgag agtaaagtac taccaaaaac atcatgtcct
     2521 tctcctgata aatttttaga agcataccat tgtcaggaag aaagtgtatt ggtgattaat
     2581 ataagttcag gattatctgg gacttataat agtgcacttt tagcaaaaga tatgtttgaa
     2641 aaggaaggaa ataacaaaaa agttgaggtt atagattcac ttagtgggtc aattggaata
     2701 ggacaattag tgttaaaagc agcaatatta ataaaagaag gaaaagaaat tgaagagatt
     2761 gtttcaataa taaataaata taaagataat cctccatttt ttggaacact tgaaacttta
     2821 gaaaatgcta ttaagggtgg tagaataaat ccaatagcag gaaaaattat aaataccttg
     2881 aattttaagg cgataataca agttgcagat ggcgttgtaa ctcctataga taaggcaaga
     2941 ggtgaaggaa acagtttgaa aaagctgatt actttagtag agtcaaaaat taaagataaa
     3001 gaagaaaagg ttttatttat aggacatgct aattgcccag aaaaggctca aaaggtaagg
     3061 gaagttatgg aaaaggatgt aaaatacaaa gatgttgtaa tctgtgaaat tggttcagtg
     3121 atggggacat atacttcaaa gggtgcaatc ttaataactg caatataaat aatggattga
     3181 aataaatcat agattgagaa attaaagcca tataaaaatg tttaaaacat ttttatatgg
     3241 ctttttaatt ttatcaaatt gattttagaa ttaaccaact acacatattt ctttgtcatg
     3301 tttgtttaat atctcacaac catcttcagt aacaagtact aaatcttcta ttctaactcc
     3361 gaactcacct ggtaaatata tacctggctc tatagagaat atcattcctg gttgtacagt
     3421 atctgtattt atagaagata catctccttt atcatgaact tcaagtccta tagagtgacc
     3481 agttctatga gtgaagtact gtccatatcc cttttcagtt atgtagtcac gagcagcagc
     3541 atctatatca cagaatctaa cacctggttt tacaatagct tcagctctct tattagcttc
     3601 aagaactatt tcaaatattt ctctaccttt ttcaggtatt tctttataga atacagttct
     3661 tgtcatatca gcacaatagt tatcttttat acaacctata tctaatatta ttgcatctcc
     3721 tggtttcaca agtgcatttc caggttcacc atgtggatta gctccattag gtccaaaacc
     3781 tataataggg tcaaatgaaa atccatcagt acctaggtct tcatatattt tagataattt
     3841 accaacaagt tgtttctctg taagttcacc agaaacagta gctttaagtt gttccatagc
     3901 cttgtcattt aattttgaag ctattctcat taactctttt tcttcttcat ctttacacat
     3961 tcttaaagta tctattatat aagatgaatt tacaaactta cttcctccac ctaattctat
     4021 taagtttaat aagaatctag ctggccagtt tttatcaaca cccatagtag catttttgtc
     4081 tatatgttca gttattattt ctactggatt ttgagtatca ttaaaccaaa ccatttcaac
     4141 acctaaatct tctgaaactg ggaataattc atttataaat aatttattat tgccatttaa
     4201 atttaagtat aatgctaata atctttcacc tggatggatt agtactcctg taagataaaa
     4261 tatagaagta gggtcactta ctaacatttg agaaatatca tcttttttca tttgttcaag
     4321 tacagcattt aatctttgag ctttcataat aatttcctcc taatcaaaat tcaatacaaa
     4381 tcaattttag cattattaaa caaaaaagag tatacttttc ttctagaaat ataaaaaaag
     4441 tttgttatat attttttata ttaagtttaa tctctacttt tgcattaaat acggaagtaa
     4501 aatgactttt tattgtgaaa attctataaa agtaataaat taagtctata aagttatgta
     4561 ttttatcttt ttatatacta atgaaaaata ataaataaaa ttttataata aaataatatt
     4621 gaaacaattt taaaaattta gtatcattag aattaaagtg gtaaaacttt taaatttatc
     4681 atttttaata agatttgatt tcaaagtatt taaggtttat tattaaaact gtttttgaat
     4741 atgcaaatat tttgaaaaat atcagtttaa taataatgaa tctattttat taattttgaa
     4801 attaaattta aaataattaa tagagagggg aaatgaaatt aggatggaaa agaaaaaaat
     4861 aggcctatgg agtttggtat ttatgaatgt atcagcttta tatggtataa gatggatagc
     4921 taagtctaca gcatcaagct ttggtttagg attaggtgca atacctatgt ggttattatt
     4981 ctcatttata tactttgtac caggagcttt aatttgcgct gaattagctt ctacatatca
     5041 aaagggtgat ggaggtcttt acgattgggt taaggaagcc tttggagaaa agtatggatt
     5101 tttagtttct tggttaaact ggacagctaa aatattttgg tattcttcat ttttaacttt
     5161 cttagcagta aatgtttctt atacaatagg aaatccagct cttgctgata ataagatgtt
     5221 tgtacttata ttatctttaa taatattctg ggcattatct cttatagcaa ctaaaggaat
     5281 aagttttaca aaaatattta ctaatacagg tgcattaggt tcaacaatac caagtgctat
     5341 acttataata ttttcatttg tagcagtatt tatgttgaaa aaacatgata tagcttctac
     5401 atatacaata gcttcaatga ctccaaaact taatatggat gcatttgttg ctatatcagc
     5461 tataatgttt ggtttagcag gagcagagac tgctgctaac ttcataacag aaatagataa
     5521 gcctgaaaag aacttcccta aggcaatatt aatatcagct ggaatagtag cttcattata
     5581 tgtattaggt tcaattgcaa taacaatgat aataccacca gaccaaatta ctgcttctaa
     5641 aggtgtatta gatgcacttt ctgctgtttg tacgtcttta ggaattggtt cttggttggt
     5701 tcaattaata gcttttggta tagctttctc agtattagga gcaatagttc tttatatagc
     5761 atcaccaata aaaatgttat ttggtagtgt taaaaaagga atatttccag atagtttggt
     5821 agaggtaaat gaacacaaga taccttctaa agcagttata cttcaagcaa taatagttac
     5881 tataatacta ttggtgacta cattaatgcc ttctgttgat gctatataca atgttcttgt
     5941 aactatgaca gcattaactg cattattccc atatgtatta ttatatgctt cttatattaa
     6001 actaagaaaa gagagaccag atgaaattag accttatact atggcaaaat caacaagtac
     6061 ttgcctaggt ttagcaaaaa tggttttagt tgtaactgta gttggtatat tattatctgc
     6121 agcaccagtt atgccgacat ttgctgaaaa tgttatatat gagatagaga tgattggtgg
     6181 aggtcttcta gttatattat ctggtctatg gttatggaat agatatgaaa agaaaacagc
     6241 taaataaaat gtattttaaa aatttctttt ggatattata atatatagtt gacaatttat
     6301 agtgtaagga atattataaa ataaagaaat taataaatta aatcctgaga taagaaatag
     6361 taaataaata tatttttgca cagagaatta aggatgctga gaacttaata aaaagtattt
     6421 attgaatggg tctttgagga gtaaaatgaa atgtaattta agattacaga gtatttgata
     6481 tcgtgtatgc acgttatagc atatattaag atatatatat tcagttttga gtgtatataa
     6541 atttaggtgg taccacggaa aatatatccg tcctattgat ttaggacgga ttttttaata
     6601 tagaaaattt aggaggaaaa atgaacgtaa taggaattat aggtgcaatg gatgaagaag
     6661 ttagtatttt agttgattta atggatatta gagagactat aaaaaaagct agcttagaat
     6721 tttataaggg aattttgaaa ggtaagaatg tagtgttagt aaagtgtggt ataggtaagg
     6781 ttaactctgc tctttgtgcc cagatactaa taagcgaatt taaggttgat gctattgtta
     6841 atacaggtgt tgcaggagct ttaaatgaaa aacttgatgt taatgatata gtaatatcaa
     6901 ctgatgctat acagtatgat gtagatacta cagcatttgg agaccctaag ggagttatac
     6961 caagaatgaa gacttctgta tttaaagcag atgaaagact tatagatgct gcttacaaat
     7021 cttctgtgga agaagtaaag actcataaag tattaaaagg aagagtagtt acaggagata
     7081 aatttataaa ttctaaagaa ttaaaagaag aattagtaaa tgattttggt ggatattgtg
     7141 gagaaatgga aggtggagct atagctcatg tatgctattt aaataatact ccattcgtaa
     7201 taataagagc aatgtctgat aaggcagatg gaagtgctga tgttacttat gatgtatttg
     7261 tacatgatgc tgccaataac tctaaagaca tagttttaaa tatgttaaaa tcaatataaa
     7321 tatataagga ggtacaaaaa aatgagtgaa aaaaaagtta tatttagtgg agcacagcca
     7381 tcaggcaaaa tgactttagg aaattattta ggagctataa aaaactggac tgaacttcaa
     7441 gataattatg attgctatta tagtatagtt gatttacatg caataacagt acctcaggac
     7501 cctaaagttt taagagcgaa tgctatagaa ttacttgctc aatatatagc ttgtggatta
     7561 gacccagaaa aaaatactat atttatacaa tcacatgtca aagagcatgt tgaacttatg
     7621 tgggtattga atactatgac ttatatgggt gaattaagta gaatgactca attcaaagat
     7681 aaatcacaaa agagtgaagc aaatctaaat gcaggtttat tcacatatcc agttcttatg
     7741 gccgctgaca tattgttgta tcaaactgac ctagttccag ttggagatga ccaaaaacaa
     7801 catttagaat tagctcgtga tttagcaaat aggtttaata atagattttc acctactttt
     7861 gtagttccag aaggatacta cccaaaaggt ggagcaagag tgatgagttt gcaagagcca
     7921 actaaaaaaa tgagtaaatc cgattcaaat gaaaatgcat ttatactatt agcagatgat
     7981 tcagattcta taaagagaaa gataaaaaga agtgttactg attctttagg tgttgtaaaa
     8041 tacaatgatg aacaaccagg tattaaaaat ctaattgata tatattctaa tttaagtaaa
     8101 aagagtgtag aagagattgt aaacatgtat gaaggaaagg gttatggcat atttaaagaa
     8161 gatgtggctg aagtagtatc agaagcttta agacctataa gagaaaaata tgttgacctt
     8221 cttaataata aagattattt agaaaaaata tatagtatgg gagcagaaaa agccgaaaaa
     8281 caagctagaa aaactcttag aaaagtttat aaaaaagttg gatttataga gagaagatac
     8341 taattattag ctatctttac ttgataaata aattttaaag ttattaaaaa ggtatatgat
     8401 atgaaagaga gagtctcaaa gtgaactttt gttcatgaga cactcttttt tgtatgaaat
     8461 aaaatatatt ttaatcactt caacaatgaa aaagaggatt atctctattt tgagacagca
     8521 tcttttgata ataaaaaaag atgagctttt gctcatctca aaggggttag attattctta
     8581 ctttggggaa agtagaataa aggtaaaagt taactgatgt caactttgtc ttttttgcat
     8641 atcactactg agcataaaaa aatcagaaaa ccatagcctt ctgaaacaac ttctaacata
     8701 cattgtattt tatacccaat atgtaattta tgagttaatt ttaactctta taatggaaat
     8761 tgtcaataat atatcaaaaa aatatttttt aaatttatat aattaaatga atttatgttt
     8821 atatataggt gatatgatac agataattat ctttataata caatatagtt ctaaaaaaga
     8881 atatgttaga agaagagaat atttaaataa aaaatacctt ataaagttaa ttttttagta
     8941 ctttataagg taaatatata ttgtattgtg gggacaaatt taaatcattt gatactaatc
     9001 aacactaaga actaataact ctctatcgtc tggagtaatt gcatatattc ttaatttctt
     9061 gtatgtcata atattttctc cacctgtaaa ataactccta agattaacat aattagtttt
     9121 aggctgattt ggatctccta aataattacg catttcttta tctttagttc caatatcttt
     9181 aaatccagaa tattgtaaag tagcttcaac tctatatttt tggatataat cgagagcttc
     9241 tttattagtt tgtgttggtg caaaatacat accatttata taagtatttc ctgtacttgg
     9301 attaaaattt aaatctgcaa aatatatttt atgtgcatcc attatttctt ggtcagttgg
     9361 aattttaact tctggttcat caagtatttc aggagtacta tttagctctg taatagataa
     9421 gttatctaag tatgttgtac cactaccaat taatgttatc tctatattag aagaatcttt
     9481 ttcagtagtt tcaaattcat aactaaattt tgtatatcct tgttctggta ccaaataatc
     9541 cgttttctct tcttttgctt ttatctttac aattattgaa ttagatgtta aaggatcctt
     9601 tgaatatcca ctaaaaacat aacgtttata aggttttagc tcagacatag ggattttaat
     9661 cttcgtctca ccatttaatt tatttgctga gccttgtaaa ccatcttgat tcgtagtatt
     9721 gacattactc catgtactag ggtaattatt ataatcatca aaattagtaa agtaagttgg
     9781 tgtttttata agtatattca ttcctctttc aagtttaaca ttatatatct ttttatcaga
     9841 caaagttttt aaactatctt taatcttatt ggctgtatta tcatcaaata tgagttcaac
     9901 acaactttca tctattggta tatcattaaa atataacaaa ccatcttttt tagtagcgcc
     9961 aaaagctttt tcaattgctt ctccaattgt aagttcaggt gttttatctt ctggatcctg
    10021 taaattttta gcagtaactc ttctttcgta agattcattc tctgtatcta atataataga
    10081 tgcagaaata ctgtcaattt gacttatata gtctgaccaa ctatttcctt ctgttactat
    10141 ttgtccagaa ctattttttg taccaaaatt tccacttact tgtgttgttt ctaatttaat
    10201 ttgctttcca gcatctaatt tttttaattg atcataattt ataggaatca gtctagagct
    10261 aaattgatcc attgtgttaa gagctagagg tgaaagccct tttttgggat aagtatctcc
    10321 aggagataga ttattgccaa tttgattttc ttgtgctttg atagttgata atgtatctcc
    10381 atctaacact aaatttgttg ttggtgtcac tttgtacata ggtgcagtac ctgtgttgta
    10441 atatctaaca tttgcattta tatatgcaga ttctcctttg tttatactta atccagtatt
    10501 ccatgattct ccattactat cttgaacagc agttgaatta tctgttgtat gggaataatt
    10561 tgtagttaca ttagctgtga atccattttg atatcctaca ttaacagaca caccagctgt
    10621 attagattca gttttactgt tagtagtagc tctggaaaca gttttacctt gatcagtaga
    10681 ggcatgttca tttgtagata taattaattt ttccatacct actccaacaa ttggatatgc
    10741 tgcaactaac ggatctcttg cttcagtctt tatagccttg tcaaaagaac ctgaagcttt
    10801 ttcataatct gtatatggat ctccagcagt atttgactct aaataatttg atacatattt
    10861 cttatagcct tgttctgcaa aactatcttc ccacttaact gcaattaagt ccttaatagt
    10921 atatccattt cgttcatatg aatctggtat attatcatta tctgtatcca aatcttcgtc
    10981 ttcccaatca gacatcaact ttgggtcaaa gaaattgtta tttgggataa atggatcatc
    11041 tttttctata ttagaataat ctcttaagaa taaattttct tctggtataa ttttcttcat
    11101 accatctaat tcccaataaa gattaggtga tgataaatta tctattgaac ctaaattttt
    11161 atcttgtagc tctattctaa ctttatattc tttacccttt ttcatattaa ctttaagtgt
    11221 atttgatata gtactctcag tatttacttg cattaagaca tcatctctat cagttgataa
    11281 tgtatattca ccatccttag aaggaattat tcttcctgtc catcgtatag attttacatc
    11341 tgatttgtct ttatccagaa gtttatctac tttcttttct tcaaatttta aattaccatc
    11401 ttttatgggt gccattaatt ttaaatcttt aaagtgctca tctgtgaaat aatatcccat
    11461 taatccattg tttgggagta tatcttcatt tacaatttct tttttcttat tagaatgatt
    11521 acttgtacta gtttgagcat atacaggata tactagtgct tgactaacta tagctgtaag
    11581 tgttaaaaaa cttaatacct ttttattcct catttgtatt ttcattgttt ctcctcctta
    11641 aatttgaaaa atattaaatt atttatgtga aaaaatatta aggtatcaat gttgcatcaa
    11701 caattaattt tgttatagta ccatctttgt aagaatcaat tttattgatt ttaaatttgc
    11761 ttccatgatt taaaagcact tcatattcac ctgcataacc tggaatagct gatagataag
    11821 ctccaggaga acctttaggt atagttatac gtagtactat ttttctttta gcaaatgcac
    11881 tcatattcac actaccaata ctagtactaa taaagtttgg ataagacagt gcttgtcctt
    11941 cccattttga tttaaaagca tctatatttt ctagtttgtt aaaatcatat tcaggggaag
    12001 taagagttaa accaaattct tgaggaccag atcttctata tacagttaaa ttagttggaa
    12061 taggttcacg ttttaatgca ttttcaatgt ttgtgatttt agaatctaat tctgggttag
    12121 gattatttac tggaccattt gatattaaat aattattaat tgcagtatat cctccacgca
    12181 tataatcatt tacatcagca agttcattag gtgttaattt attactccaa tcattataat
    12241 ttgctttacc ccaagaatcc cccttactta catcatcttt aaaatcaaga ctacttacaa
    12301 cagatgcttc tgctttaata tagtgcttcc catctataac tatacgaaca attttatcta
    12361 tttttatact atatccttgc tctattaatg tacttacatt gttagtattt gtatatggta
    12421 acataccagt atttctaggt aattttaagt gcataagtaa tggtgtaggt ttttcatcac
    12481 cttttccagg ttcatataaa gaaatatctt taaatccatc ttgcttaaat aatttgtttt
    12541 gtatagtttc tttaaactca ttaaattttt ctaatgaaat ttcgttttga ttttctgttc
    12601 ttattacttt attaaatgca aatttttctg gagattcaaa ataatagaca tacataggtt
    12661 tatctatttt attttttgat atagcatttc gaagttcttt atattctttt tctcgagaat
    12721 ttgcttctat ttgataatca taaaaataat ttcttgtctg agaatattta cttatttcta
    12781 cagaatcttt tttatatgat tctaatgctt ctttttcaga tctttcaagt ttttgctcta
    12841 ttctttctgc ttcttttctt tcccattctt tagccttttc tttatcttta agaaaatctt
    12901 ctggtctttc tataggagcc ttgtaagtag tattgcaaac tttttcacta tagctttgta
    12961 agtcttgtgc ataaatgtta ttaggtaaca aaccacctaa agttagatat aatatcaaca
    13021 atatagatat acaattacta atccttttat gtttcctaaa ttttttcatt tattctccct
    13081 cccaatatta gtttaattaa ctcttgaact ttaatttatt tagattatat aatctaaata
    13141 agaatttttt atataaattt cataaaatat gtaactatat atcataaaaa tatgtattta
    13201 tatgatatgt cagtacaaaa taacaactga atattttaaa gagactattt catactttta
    13261 tgatttaatg agctatcttg ttgaatcata atctaggaac aatggttgca atgtctaata
    13321 gaaattgtat atgatatagt cattatgtgt tttttcacaa aacgattatt atatagagga
    13381 ttcttaggaa ctactttttt tgtataaaaa aagagatggt tcaaattctt atcttgagac
    13441 atctcttttt tctatttatt atgttttaat aatgttcttt aaaatatttc tatatgtatt
    13501 agatatacga catatatggc cattactcat tttaatatta tttttcttta ggtcgagaga
    13561 aacaatatga tatttattaa ctacaaaaga tttatgtaca cgaataaagc atgcatctaa
    13621 atctgataaa gaccttaaac ttttatagaa ttcaaatatt cttgaagtag tcactaattt
    13681 tattttatgt ggaatagcgc ttgtttcaaa atatataact tcatcaaatg aaattttcca
    13741 tagtgaggaa tctgagtaga tagtaaaaaa attagagcga taattttttt ctttaacgta
    13801 tcttttatga gcaattttaa tacaagcttt tattttactt tcagcaattt ccatatcatg
    13861 ttttaatata aagtccataa cttctaacct ataatcgaag atccctaaaa tcatttctat
    13921 atgatttgta agaaatatga ttggactaat ataatctgat tctctaattt tttgtgctat
    13981 ccataatcca tcacataatt cattacttat cttgtatttt gcatctacaa ctataaaata
    14041 tatttttact ttattttttt cagtaagttc ttttaata
//
LOCUS       DAGQWR010000107        14076 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.107, whole genome shotgun sequence.
ACCESSION   DAGQWR010000107 DAGQWR010000000
VERSION     DAGQWR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.107"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            448..1341
                     /locus_tag="MIW95_002790"
     CDS             448..1341
                     /locus_tag="MIW95_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBY3152102.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     ILKLEEGDSHHHSNCGCGGNK"
     gene            1341..2735
                     /gene="gltA"
                     /locus_tag="MIW95_002791"
     CDS             1341..2735
                     /gene="gltA"
                     /locus_tag="MIW95_002791"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBY3152103.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLNINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKTKVNA"
     gene            3101..5320
                     /locus_tag="MIW95_002792"
     CDS             3101..5320
                     /locus_tag="MIW95_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY3152104.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5467..6213
                     /locus_tag="MIW95_002793"
     CDS             5467..6213
                     /locus_tag="MIW95_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152105.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PTILLADEPTGNLDSKSTDDVLKLFTRINKEQNVTTLMVTHEVYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6213..8093
                     /locus_tag="MIW95_002794"
     CDS             6213..8093
                     /locus_tag="MIW95_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152106.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELTILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEIYFPVKAILLTIVS
                     FMILFLITGPMSLKLLRKKSILELLVGTKKPKKEIKSSRLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYDEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFRLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            8212..9603
                     /locus_tag="MIW95_002795"
     CDS             8212..9603
                     /locus_tag="MIW95_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY3152107.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9710..10750
                     /locus_tag="MIW95_002796"
     CDS             9710..10750
                     /locus_tag="MIW95_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBY3152108.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11239..11892
                     /locus_tag="MIW95_002797"
     CDS             11239..11892
                     /locus_tag="MIW95_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBY3152109.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIHGIIDGMKF"
     gene            11968..12177
                     /locus_tag="MIW95_002798"
     CDS             11968..12177
                     /locus_tag="MIW95_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152110.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12317..12883)
                     /locus_tag="MIW95_002799"
     CDS             complement(12317..12883)
                     /locus_tag="MIW95_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBY3152111.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSP
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13044..13697
                     /locus_tag="MIW95_002800"
     CDS             13044..13697
                     /locus_tag="MIW95_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBY3152112.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13855..14031)
                     /locus_tag="MIW95_002801"
     CDS             complement(13855..14031)
                     /locus_tag="MIW95_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152113.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5520 a         1388 c         2370 g         4798 t
ORIGIN      
        1 aagagtatat tttatataat atgaaagctg gtattgcaat agattaaagc cttatacacg
       61 tttagttttt aaagaaatta gtgttattat gaaaacctaa gttaatttat taaataaata
      121 attgaataaa aaaattttat tatgtattta taaaattttt gttacaaatt tggcataata
      181 tagtatactt taagtgtgta ttaggttaaa tctataacaa attagacgta tgatatagac
      241 ttagtgttta ttggcattta caagaaattt gttaatatca actatttgta gtttgggcag
      301 ttataattac tatagggggg atgtccaata caaaacaatc aaccaagaac taatattata
      361 ttataaagag ttatagtttt gtgtgtttat gtttttaatt aaatttaaag tgggaaaaat
      421 atatttgtta gaatggagaa tagcgagatg agtaataaaa tagttagtaa gagacaatta
      481 acagatagta tttatttaat ggaaatagag gcacctagag ttgcaaaatc ttctcaacca
      541 ggtcaattta taataattaa aaatgatgaa aagggagaga ggattcctct tacaatagct
      601 gactatgata gagaaaaagg aacagtaact attgtattcc aaacagttgg tgcttcaact
      661 aaaaagttag caatgtttga agaaaacgat tttgtaatgg attttgtagg gccattagga
      721 caagcaagtg aatttataca tgaagatata gaagaattaa gaaataaaaa gatattattt
      781 gtagctggtg gagttggttc tgctccagtt tatccacaag ttaaatggtt taaagagcat
      841 ggattagatg tagatgtaat aattggtgca agaactaaag aattaattat attagaagat
      901 gatatgaaaa aagtagctaa aaatgtatat gtatcaacag atgatggtac atatggattt
      961 aatggaagag ttacagacct attaaaagat ttagttgata atcaaggtaa aaaatatgac
     1021 caagcaatag taattggacc aatgataatg atgaaattta tgtgccaatt aactaaagaa
     1081 ctaaatatac ctactatagt aagtttaaat acaataatga tagatggaac aggtatgtgt
     1141 ggtggttgta gagttagtgt tggaaatgaa actaagtttg catgtgtaga tggtcctgaa
     1201 tttgatggtc atttagttga ttttgatcaa gcaatgagaa gacagtctat gtacaagact
     1261 caagagggaa gagctattct aaaacttgaa gaaggcgata gtcatcatca tagtaattgt
     1321 ggatgtggag gtaacaaata atggatgcta aaaaagtaaa agtaccagtt agagaacaag
     1381 aacctgctgt gagagcaaca aattttgatg aagtatgctt agggtataat aaagaagaag
     1441 ctatggcaga agctaataga tgtttagctt gtaaaaaacc aaaatgtgtg ggtggatgtc
     1501 cagtaggaat cgacatacca gggtttataa caaaaattaa agaagatgat atagaaggtg
     1561 ctgctaaagt aatagctaag agcagttcat taccagctgt ttgtggtaga gtatgcccac
     1621 aagaaagtca atgtgaaggt gtatgtatac ttggaataaa atcagatgct gtatcaatag
     1681 gtaaattaga aagatttgta gcagattggt caaaagaaaa tgacataaat ttatctgata
     1741 cagagcctaa gaaaaatcaa aaagtagcag taataggaag tggtccagca ggtctagctt
     1801 gtgctggtga tttagctaaa aaaggctatg atgtaacaat ctttgaagca atgcatgagc
     1861 caggtggagt tttaacttac ggaatacctg agtttagact tccaaaacaa gctgttgttc
     1921 aacctgaaat agacaatata agaaaattag gtgtaaaaat agaaactaat gtaattgttg
     1981 gtaaaacaat tacagtagat gaacttatag aagatgaagg atttgaagct atattcatag
     2041 gttcaggagc tggtcttcca atgtttatga atataccagg ggaaaatgca aatggagtgt
     2101 tttctgcaaa tgagttttta actagagtaa acttaatgaa ggcttataga gatgactatg
     2161 atacacctat tagttctggt aaaaaagttg ctgtagtagg tggtggaaat gttgctatgg
     2221 acgctgctag aactgcatta agattaggtt cagaatcata tatagtttat agaagaagtg
     2281 aaaaagagct tccagcaaga gctgaagaag ttcatcatgc aaaagaagaa ggcattatat
     2341 ttaatacatt aacaaatcct aaggaaattt tagtagatga aaatggatat gttaaaggta
     2401 tggtttgtat aagaatggaa ttaggagagc cagatgattc tggaagaaga agacctatag
     2461 aaatagaagg ttctgaattt gtacttgatg tagacacagt tataatgtca cttggaacaa
     2521 gcccaaatcc actaatatct tctacaacaa agagtcttaa tataaataag aagagatgct
     2581 taataacaga tgaaaatggt cagacttcta aagagggtgt atttgcaggt ggagatgctg
     2641 ttactggagc agcaacagta atatctgcaa tgggtgctgg taaaacagca gcagcatcaa
     2701 tagatgaata tttaaaaaca aaagttaatg cttaatttgt aaattaaaga gagggtgtct
     2761 caaaatgaac tttttagttc atgaggcact ttttttgtac aaaatcaaat atgttttaat
     2821 catttcaaca atgaaaattt cattcctgta gaaagtctaa cttttaagtc ttagtacaat
     2881 ctctattttg atgtaggcac ttttttagat ggtttaataa tttatcagtt attattgtaa
     2941 tatttgatac taagtaacat aactttgtct aactatgata aaatagttat acaataaagg
     3001 tcttgtaagg agtgaataat gtgtggctaa taaaatcaga tttgtcaaat ctaacattaa
     3061 ataatagaaa aataaattgt gtaattaatg gtattttaat atgaaaaaaa gagaaaaagt
     3121 atttgatggg ctagttttta agcatatatt aatcttatta atactattag ttttgattga
     3181 tttaacaagt accatcatat atgcaagtaa ctctatggaa attacttcta gaaatatgat
     3241 tgaggcatca aaaagagaac ttgaaaacta tttagaagta aacatatctt tattaaaagc
     3301 tcttagtcaa gatgatagat tttctaatgg tgaaacatca ctaattgaaa agggaaaatt
     3361 attaaggcca tatcaaaagg aatataatct ttttatgatt ggtataacag atacaaaagg
     3421 aaatgcttca agtacatata gggaaaatgt taactcaata aaagataagc ctagttttga
     3481 gaaagcaatt aaaacaaagc aagtagtagt ttcagatatt gaagtcagta atgtaacagg
     3541 tgacaaagta tttatcatat atgtaccaat tgtaaaaaat aatgagatga ttgggaccat
     3601 ttttgcttct ttttattttc aagatgtaaa taatttaata agtagaagca attttgatga
     3661 ttctattaaa tttctaatga tagataaaga ttatactatt atttctcatc caaacaaaaa
     3721 atatgtaaat gataaaagta agatgcttga tttagaaggg aatattatag gaaccactaa
     3781 aagtgaaatc ttaaaaaata taaataaaaa atgtcaaggt gattttttat cttgggataa
     3841 ttggagatta tataatgtaa aatatacgaa cataaagtgg actaattgga ctttagtatc
     3901 aaaatgtaat atatttaaga actttaaaaa tctaatagtt aattttatga taaagttgta
     3961 tttttatata gtaatattta tgatattgtg gaaattaagt aatgctaaat taatagaaca
     4021 acttaaaaaa ttggcttatt atgattcttt aagtggaata aaaaataaag aaaagtttag
     4081 aaaagattct atgtatattt taaagaatta ttatcaagat aatttttact tagtacaatt
     4141 ggatgtcaat aaatttaaat atataaatga gatgtttgga tatgctgaag ggaataagat
     4201 attaattcat atatcacagg ttttaaataa taatacaaat aaatatgaaa tttgtgctag
     4261 gatggataat gaccatttta tattgttgat tgcttgtaat actgaagatg aacttttaaa
     4321 tagattatct aaaataaata aagaaatttg taacttaaat actactaact catctaagta
     4381 taaaattgtt atgtcaagcg gaatatataa gattactaaa aaggatgata ttaaaaagat
     4441 tgacctgtta attgatagag caaatattgc agcaaaaagt aaaaaggaaa aatatgaaca
     4501 ttcatattcg ttttttaatg aagatacaag aaatcgattg tacaaggaaa aacgattgga
     4561 agataatatg aataaagctt tagaaaaagg tgaatttata gtatattatc aacctaagta
     4621 tagtttagat gatgtaaatg aaattgaagg agcagaagcc ttgataagat ggaatagtcc
     4681 agaatttggg tttatttcac ctatagattt tgtaccttta tttgaaaaga atggttttat
     4741 tgtaaatatt gatatgttcg tgtttgaaga agtatgcaaa acattaaaca agtggataaa
     4801 taaagggtat actcctgtac caatctctgt aaatatgtct agagttcatt tatataggga
     4861 taattttata gaaaatataa ctgatttgat aagtaaatac aatatatctc ctgagtttat
     4921 tgaacttgaa ctaacagaaa gtgttgtgtt tgataattta aatatattga ttgatataat
     4981 gaaaaagatt aaagagatag gattccttat atcaatggat gattttggtt cgggatattc
     5041 atctttaaat ctgttaaaag acctttcttt tgatatttta aaattagata gaggattttt
     5101 aatcgaaaca acagatacaa aacgtggaaa aattattatt tctaagattg tggaaatggc
     5161 aaaagcgatt gatattaaag taatctgtga gggcgtagaa acttatgaac aagtagagtt
     5221 cttgaaagag ataggttgtg ataaggttca aggatactta tttgctaaac ctatggtatt
     5281 agatgaattt gaaaaacact taaactttaa atttgattaa tcattaataa ttattagact
     5341 ataatatacc ctaacaactt acttacaaat atgtaagtta attgttaggg tatttttatg
     5401 gttaaagtag tatattcacg atattatggt attgtagtaa aaataaaaaa ttatggagaa
     5461 tataaaatga acatattgag tattagaaat atatcaaaaa catattgcgg aaatattcca
     5521 tttaaagcat tagataaggt tagtctaaat atagaaaagg gagaatttgt gtctgttatg
     5581 ggaccatctg gaagtggtaa atctactctt ttaaatataa tatctacagt tgatagacag
     5641 agtgaaggtg aggttgtatt agatggatat gatgtaagta aattgaaagg agaaaaactt
     5701 gctgaattta gaaggaaaca acttggtttt gtatttcagg attttaattt gattgacact
     5761 ttaactgttg gcgaaaatat tatgttgcca cttacactag agggggcaag tatcaaagat
     5821 atgaatatac aaactaaaag tatatctaaa tttttaggaa tagataaaat tttagataga
     5881 aaaacctatg aaatttcagg aggtcaagct caaaggtgtg ctattgctag agctattata
     5941 aataaaccta ccatacttct tgcagatgag ccaactggaa atcttgattc aaaatctact
     6001 gatgatgttt tgaaattatt tactaggata aacaaagaac aaaatgtaac aacacttatg
     6061 gtaactcatg aagtatatag tgcaagttat tcagatagag taatttttat aaaagatggt
     6121 tgtatatata cagaaataaa aaaatctgaa agcagtaatt ctttctattc tgatatacta
     6181 gctgttttgt ctcaaatagg aggtgtaaga tagtggattt tagaaagttt gcttataata
     6241 atatagttag aaatttcagg gcatatttag catatttttt aagttctgtt ttttctataa
     6301 tgatattttt tgtttttgca gtgtctatgt tccatccgat tataacaaaa tctggaatgc
     6361 agagtggttc tactgcattt atggctctta tgacttcaga attaacaata ttaggtttta
     6421 gtctactttt tatattgtac tctttaggaa atttcttgaa atatagattg aaagattttg
     6481 gagtattgat gataattgga atgagcaaca gacaactgaa aaaattgata ttaattgaaa
     6541 atttgataat aggattttta gcagtgatgt taggaatatt acttggttta tcaatttcaa
     6601 aattgttttt attgtattta agcaaactgt tttatatgaa tttaactgaa atttattttc
     6661 cagtaaaagc aatactattg actattgtat catttatgat tttatttttg ataacaggac
     6721 caatgagctt aaaattattg agaaaaaaga gtattttaga gcttttagta ggtacaaaaa
     6781 agcctaaaaa agagataaaa tcttcaaggc tatttggaat aattggtgta atatgtctta
     6841 tattaggata tgctatgata ctatttggca ataagttaaa aataaatgga ggtatgtata
     6901 caacagcact attaattact ttggggatat ttttattttt ttctcaattt actattttga
     6961 tattaaagta tttaaaaaat aaagaaaaat tttataagaa caaagtaaat atgttactta
     7021 taagtaattt gatatataaa ttaaaggata atacacgatt gttattttta ataacaatac
     7081 ttttatcggc tactttagta gcatttacaa caacttcaac acttgtaagt tctcaaggag
     7141 aagcttctaa aaataatttc ccaatggttt actcatattt ttcagagcat aataatgaaa
     7201 aagaatatga agaactacaa caaattagag aagcaatcaa aggatatgat tataaggagt
     7261 taagttttcc tgtacttaaa tataataggg aagtcttact aagtgtaaag aattataaca
     7321 aattatcaaa ggaattggga ttagataaaa ttagttcaaa gttagaaaaa tatgaaggga
     7381 ttataattcc tagtagcaat tcacaagaac atataaattc tttaaaacaa ttaaaaaatt
     7441 atagtataaa aaatttaaat atagaagtaa aaaaatcaaa aaataaaata atttttccta
     7501 caggactgtt tagtaaagtt gtagttataa gtgatgactt atacgatgaa gtaaaaggtg
     7561 aattttcaaa aattaatttt catggatttg attataaaaa ctggcaaagc agtgcaaaga
     7621 taactgaaaa tttaaaagaa aaacatttta atttggatag agaatataac aggtcatatt
     7681 ttttaacatt accagatatg tatttagtag agttacaaca aagtaaaatt cttcaattta
     7741 tgggtgtttt tataggagtg atattattta tagcagtttt tagtttttta tacttcagat
     7801 tatatacaga cgagatagaa gataatgtaa agtacaaaaa actatcaaaa ataggtctat
     7861 cttttaaaaa tatgaataag attgttagta tagagatagg tacattgttt tttataccgt
     7921 attttatagc aattataaat agcataattt ctttactgtt tttaaatcaa gtattaaata
     7981 atagttttag gttagaaaat ttgggtatat tactactgat atcatctata tattcacttt
     8041 atttttactt attaaaaaaa atttatacta gaaaagtttg gttttttgat taaagtggta
     8101 tataatagat atataaatta atatttaaaa agtagttaat attaggggag ggcatttgca
     8161 cctcccttgt aatatgtgga aaattgaata ttagaagaag gaggagactt gatggaaaat
     8221 caacaattat taggaacaga acgaattagt aaattactac taaaatattc tataccagct
     8281 ataataggta tgttggtaaa tagtttatat aatgtagtag atagaatttt tattggaaat
     8341 ataccagggg ttggaccact tgcaataaca ggtcttggtg taacaatgcc aataatgaca
     8401 ataatacttg catttgggat gttgataggt ataggaacta ctactaccat atcaataaaa
     8461 cttgggcaag gtaaagtaga agaggcaaga aaacttatag gaaatgcaat gacgttatct
     8521 gtaataacag gaattatcat aatgatactt ggaatattat ttgcaaataa aatacttaca
     8581 ttgtttggag caagtgaaaa tactttaata tatgcaaaat catatataaa tattatatta
     8641 ttaggtacag tagtaaatct tctttccttt tctttaaacc actcaataag agctgatggt
     8701 agtcctaaaa tttcagcagg aataatgata gtaggatgtt taactaatat agttttagac
     8761 tggatattaa tatttggatt caatttggga atacaaggag ctgcaattgc tacagtaact
     8821 tctcaggctc ttacagctat tttaactata gggtattata taagtggaaa atcaaactta
     8881 cgattcagta aatctaatct aaaattagat aaaaagctaa taaaagcagt gtttgcaata
     8941 ggtatgtcgc catttgcgat gcaacttgca gcaagtttag ttcaagtaat atcaaatatt
     9001 gcacttaaaa ctcatggtgg agatttagct ataggggcta tggctactat ttcttccatt
     9061 gcaatggtat ttttgatgcc tatctttgga ataaatcaag gagcgcaacc tattataggt
     9121 ttcaattatg gagctgaaaa atatgatagg gttaaaaaag catatttagg ttcattagta
     9181 gtagctacta taattttatg tatgggtatg gtagtagtta tgcttttccc agaagctatt
     9241 ataggtatat ttaataaaga ccctgaactt atgaatatat ctgttaatgg attaagaata
     9301 tacttactta tgttgccaat tgtaggctta tcagttacag gaacaaactt tatacaatct
     9361 ataggaaaag caaaaatggc tatgttattg agccttttaa gacaagtaat tttattgata
     9421 ccagcagtat taattcttcc tacattctta ggtttacagg gtgtatggac agctcaacca
     9481 gtgtctgatt ttatagctac agtaattaca ggtatagttg tatttagaga attaaaaaga
     9541 tatactccta aaactgaaaa attaaatgaa aatgaaagac taaatgagat aactactgag
     9601 taaaaatata aattcagata attgagcaag aaattgcaaa aaatacgatt atatgataaa
     9661 ataaaaccgc tgtcaaggat aagagaaatt ctaattatgg agtgatttta tggaatttac
     9721 aaagaaaagc aaattaaaaa atatgtatgt aaacatgaat gtagctagaa tgttgtctaa
     9781 gataaatgag tataagggta ggcaactgct ttacaaaaaa caaccaaaag aaattttaga
     9841 aaacctagaa aaaaaatctt tagtagattg ttctgaatct acatacattg gaaatcaaag
     9901 agatagtagt aattttaatc tagaaaagtt gataagtaat gaagttacac ctagaagtag
     9961 agaggaattg agtattgttg aatacagaga tgtggtaaaa actattaaca gtgcttatga
    10021 gagtattccc atcagttcac aaacgattct tgagcttcat gggtacttat ataaattttc
    10081 atctactaga ggaggaagtt ataagtcaga taatgatttt attgaacaca atttaaaacc
    10141 tagcgaattt ataagtattg attcaagtgt caataaagtt gaaaaagctg tagaagaaat
    10201 atgtgaagct tataatacat tgatagagga agatgaaatt gatatattaa tattaatttc
    10261 tgcatttgtg ttagatttca tattgataca tccattcaaa gaaggtaata taaaaatggc
    10321 gagagtactt atcctattgt tactaaataa aaatgggtat gaagttggaa gatatataag
    10381 tttaggaaag atatttgatg atagttcata tgaatattat agtaatttaa attatttgaa
    10441 agcctcaata ggtagtgaaa aagcagatat gaatgcatgg attgagtatt ttttagagac
    10501 gatattaact gcatatgaga agctggatga tagtttaaat atatctgata agaaaagaca
    10561 aacaaaaact agtagaatag aaaaaataat aaattctacc cttggatatt ttacaaaaga
    10621 agatataaga gatttatgtc ctgacattcc agaacctact ataaatagag tttttaataa
    10681 tctaagaaaa caagataaga tagaagttgt tgcaagagga agaagtgcaa aatggaagaa
    10741 aaaatattaa aaaatgttta aataactaaa atggtatagt gttttacact ataccatttt
    10801 taatgtcatt agcatgagca ataatttaac aacttaagca atatcaattt tatttattga
    10861 ttatttttga actgaaaaat ggggttatat ggtatactac aataataatt aattttggat
    10921 gagaatatat aacgttacag aatatcttag gagtatatgt ataaaaaata taataaaagt
    10981 gtgaggttta gtaattaaca aattatgtgg cttcacactt ttttataaaa taaaaatact
    11041 tagcagaaga atattttagt tgatatgttt ataagatagt acttgtttgg gtaaaagtat
    11101 aaatagtaaa aaagtagaag taatgtgtat aaaaacataa gttacattct gtaataatag
    11161 ttattttata attcaacaaa agaatagaat gggtatattt gtgaaataat aatataaaat
    11221 aatcttagga gtgatcttat gaagaaaaaa ttattatata taaatgtaaa ttcaaaacct
    11281 gaagacttat cttcaagtaa aactgtagca agaaaattta taaataaatt tatggaaaaa
    11341 aacaaagatt ttgaggttga agagatagac ctttataaag aacatatacc gagacttgag
    11401 tatcagtact ttgaaaaaag aaatagcata gttagtgaag aaaatgctaa aaatttagac
    11461 gataaggata gaaaagagct tacaaagatt agaaatcttt gtgaccaatt tgtgagtgca
    11521 agtgtttatg taatagcagc acctatgtgg agtttatctt ttccagcacc acttaaagag
    11581 tatatagatt gtataattca agatggaaaa actatatctt ttgaaggcaa tgataaacca
    11641 caaggaattt taaatgatat tgatagaagt atggtatata tacaatcttc tggtggacac
    11701 ataccatggg tcttgaagcc agttatgaat aaagggttaa attatgtgga aagtattatg
    11761 aaatttatag gaattaaaaa atttgatgaa ttgttagtag atggaactgg aactagtgaa
    11821 gaagaaagac aagctgcaat agaaaaagca tctgaaaaaa tacatggtat aatagatggt
    11881 atgaaatttt aattataata ttttattaaa aatataaagg aaaaacctct tttaatgtag
    11941 aaacttatat tgaaggaggc gagaatcatg cgagaagaaa aaagtaatga aaagtatgat
    12001 tgttattggt gtaatcaaga aaataatttc tgtgtagaaa taaaagataa tatagtaatg
    12061 atagatgatg gcactggtac gttaaaacaa gccgttttca tagggtataa acaaatccaa
    12121 attaatttaa attgttcaca ttgtcaaaac ttaaatagaa taaaattaaa tttgtagaat
    12181 tttaaaatgg aaattattga aggaattaaa aatagaaagt gttaatattt agactgaaaa
    12241 ttatatttta tacaataaaa gtttatttaa ataacattgt attttaatat ttattttcag
    12301 tcttacttta ttaaaattat tttttggtta tgtctggaaa atatctgtct actaattttt
    12361 ctgaaatatt ttttaattgg tcagccattg aagtcagact taataaggct atcaataaat
    12421 catctttatt ttctggattt atactaatat tttcaaaatt agactctata aagtccggta
    12481 tattctttct ttgattttct tttattaaca tgaatttttc gtaacatgaa actaaatctt
    12541 tttcactgaa attatccttt tcatacacac catgtaagat tcttttaatt tcttgtattg
    12601 taagtgtatt tttcatagaa taaataatta tcatctgtaa aatttgttct ttagtatact
    12661 tttttccctt aactggtttg attaatccct ctttgctgta attatttatc atggttttag
    12721 taagaatttt atcactttca aatcttttat taggagaaag tttattgtca attagagaaa
    12781 taatttgatc catatataag tcaatagagg gaatatcgtt tgaagttata tctgcattat
    12841 ttagtgtttc tagtattata gtattaagtt cattattcat catatgttac ctccataata
    12901 tatataatac actaaattag aaagtaaaac aatattaaga cttgtaaaaa aaacataaag
    12961 tggtataatt aaatatataa tatagtaatg aaaactatat ttatgaatct gatttttaaa
    13021 atacataatt gtaggaggac gaaatgtatc aatttttttt aaagggtaga gacccaataa
    13081 gcagtttaac tcattttata ggtgcttgtt tatctctttt agcaactata attttagttt
    13141 ttcaatctgt tacattacag gaaacttctc tcttgatgat agtatctgta tcggtatttg
    13201 gactttcact aattgcttta tatagtgcaa gttcgtatta tcatttttta aaggggacac
    13261 cagaacaaga attgtttttt agaaaagtag accatgcaat gatttatgta ttgattgctg
    13321 gctcttatac accaatatgt ctgaatttta tggaaaaaaa agaaggcata atatttgtta
    13381 cagcaatatg gatagttgca tttattggca taataataaa aatattttgg atggatgcac
    13441 ctagatggtt atctaccagt atatatttac ttatggggtg ggcaattgtt tttgatataa
    13501 atgcattcaa ttcaattcct aaggattgcc ttagactatt aattatggaa ggtgtatctt
    13561 actctatagg tgcaattatt tatataatta aaaagcctaa tataagtcct gagtttggat
    13621 ttcatgaaat atttcatatt tttattatga taggttcttt atttcacttt ttagcagttt
    13681 tattgtatgt tttatagttt taaaagattt gattaattga tactaaaatt ctacaatgaa
    13741 taaaatggaa ctgccttata tagattatat cttcgacaat aagacagttc cttattataa
    13801 atattaaatt ctacttataa taaaattttg ataaaaatag tccattcaaa atgcctattc
    13861 cataattcct ttaatgtact tttcatttac gacatatagt ttgaatttat gtactataat
    13921 tatatcctct aaattctctg aaacactaac aattctgaca catttgtttc taatttctac
    13981 aggtaaatct aaatcatttg ttaaattcac atagtcatca tatttataca taaaatcatc
    14041 cctttacatt aagatatact tattatacta tatttt
//
LOCUS       DAGQWR010000108        13882 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.108, whole genome shotgun sequence.
ACCESSION   DAGQWR010000108 DAGQWR010000000
VERSION     DAGQWR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.108"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..847)
                     /locus_tag="MIW95_002802"
     CDS             complement(<1..847)
                     /locus_tag="MIW95_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBY3152114.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEF"
     gene            complement(887..1234)
                     /locus_tag="MIW95_002803"
     CDS             complement(887..1234)
                     /locus_tag="MIW95_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152115.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1452..2408)
                     /locus_tag="MIW95_002804"
     CDS             complement(1452..2408)
                     /locus_tag="MIW95_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBY3152116.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTIYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIQAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            2849..3217
                     /locus_tag="MIW95_002805"
     CDS             2849..3217
                     /locus_tag="MIW95_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBY3152117.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNKVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
     gene            3642..3887
                     /locus_tag="MIW95_002806"
     CDS             3642..3887
                     /locus_tag="MIW95_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152118.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
     gene            3986..4249
                     /locus_tag="MIW95_002807"
     CDS             3986..4249
                     /locus_tag="MIW95_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBY3152119.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(4534..5481)
                     /locus_tag="MIW95_002808"
     CDS             complement(4534..5481)
                     /locus_tag="MIW95_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBY3152120.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALATEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5484..7115)
                     /locus_tag="MIW95_002809"
     CDS             complement(5484..7115)
                     /locus_tag="MIW95_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBY3152121.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            7245..8162
                     /locus_tag="MIW95_002810"
     CDS             7245..8162
                     /locus_tag="MIW95_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY3152122.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            complement(8219..8800)
                     /locus_tag="MIW95_002811"
     CDS             complement(8219..8800)
                     /locus_tag="MIW95_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY3152123.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(8840..9406)
                     /locus_tag="MIW95_002812"
     CDS             complement(8840..9406)
                     /locus_tag="MIW95_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY3152124.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            complement(9861..10721)
                     /locus_tag="MIW95_002813"
     CDS             complement(9861..10721)
                     /locus_tag="MIW95_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY3152125.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            10870..11760
                     /locus_tag="MIW95_002814"
     CDS             10870..11760
                     /locus_tag="MIW95_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY3152126.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLSPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            11978..13732
                     /locus_tag="MIW95_002815"
     CDS             11978..13732
                     /locus_tag="MIW95_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBY3152127.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDVTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNVKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVSVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLVGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         5112 a         1874 c         1929 g         4967 t
ORIGIN      
        1 caaattcatt gagttttatt aatttgaaag aagaagatag atttttaaag ttaatgattt
       61 tagagttatt cactaataca cttctttttt taaaataaat ttctttttta cttatattta
      121 gtatagttaa tatttgttta agatatatta ttatatcttc tggagttcta tcaaatttga
      181 gttcaaaatt ttcatcttca aagtagggaa tataatataa aggtgttttg agagcaggta
      241 atttattata taattcttca agaatatcga tatttttagg ggagttaaag aaaaagcatt
      301 tacatacgtt cattaaatcc acagcatctg aaagtgagtt atgtttaata tctccttcta
      361 cagaaaaaat ttttttcata ttcagtaaag atatttgctt agataagatt ctattattgt
      421 atgtaatatg ttgagatata aaaggttgaa tgtcaatcat catattaaat aactctaagc
      481 atttacctgt atatttatat actctaaatg aagttagaag gccagttata tcaaaatcac
      541 cccaattata tattgtaaca tcatcaaatt tacaaaacca tttataaaaa tcatccataa
      601 catataaaag attaggagca ttattgatat ctagttggtt caatttggtt aaatttttac
      661 atttatttga aagtatttta ttgtattttg gcttaactaa agagtaataa ttatctattg
      721 tattaaattc gttatcaact aaaactccac caatagaaat taattccgca ccattatatt
      781 cttttggttt atttttatta tcatctagta tattaaattc aaagtctata aataacttgt
      841 atttcataaa tctaaagcac atcctcaggt aaattattta ttgagcctaa gcatcataag
      901 aatcaataga tataatgact ctaaacatca aaggttcatt acaactttta tttatttcat
      961 aatttaattc aaataaatct tttaagatat aatttataag tattctttcc caacccattt
     1021 ttaaattaac tttagagtct attaatatat tttctatctt tttaaatatt tctatatttg
     1081 ctggtattat actgagctca atattacttt taaacgggtt agactgtaaa tctgttataa
     1141 gtatattttt taattcctct gtaagttcag aaattaaact atcacctatg tattttttta
     1201 tgatttcctc tctaatttct tttaaattta gcaatattag tacctgctac ttacatagta
     1261 gcttttcctt tccttttaat actattattc atttttgaat cttatttaga tatattttaa
     1321 gattaaaagt ctttagctat ataatttttt acccattttt aacttatatt aacatattac
     1381 aggcttatat ctccaccctg cttacattga ggatggagta tattgcctat tttgataaaa
     1441 atgataggtt gttatttgcc tattcctttt tctgataatt tttctaacat atctttggtc
     1501 attttatcta aatcataact aaacttaaag ttccattctt ttactgcact actactgtct
     1561 agagagttag gccaagaatc agctatactt tgtcttacag ggtctacatc atattctatc
     1621 acaaaatcag gaatatattt ttttatagaa tcagctattt cttcaggaga aaagctcata
     1681 gcagtaatat taaaagcatt tctatggatg agtttatccg ctggagcttg gattaaatcc
     1741 actattgatt gaagtgcatc aggcatatac atcatatcca tttttgtacc ttctgctatg
     1801 taagatttat atctcttatt ttttaatgcc tcataatata tatcaactgc ataatctgta
     1861 gttcctccac caggaggcgt aacataagaa ataagacctg gaaatctaac acctctagtg
     1921 tctactccaa attttgagtg ataataatca catagtaatt ctccagaaac tttagtcaca
     1981 ccatatatag tattaggtct ttgtaatgta tcttgaggag tcatattttt tggagaattt
     2041 tcaccaaaag cagcaattga actaggtgta aaaaatttac aatccaattc tttggctatt
     2101 tcaaggccat ttattaatcc attcatattt aagttccaag cttctagggg tttactttca
     2161 gcaactgcag aaagtaaaga tgctagatga attatagtat ctacatcata tttttttgca
     2221 atttcaaaaa aacgattctt atctgtaaca tctaatatct caaatatacc agattcacat
     2281 actggattac cttcttttac tctccttgaa ctagcgatta cattttgttc tccatactca
     2341 tttctaagct ttattgttaa ttctgaacct atttgtccta atgcacctgt tataagtatt
     2401 tttttcaatt ttacaacctc ctaagggttt aatttatgta gtactactta tttttatttt
     2461 agcaaatctt ttgtttaaat acaaagaatt aaagataata atatacgact taattgaatg
     2521 aagtgcagta aattcctaaa aatttaaagt tgagatataa tggtctattg tataacaata
     2581 aaagactaga ttaaattatt atatagattg tgtgactaag tcacagaaag tatagttaat
     2641 ttaatatata ataaaagcat atcaagtaaa gctttaaatt aaagtaactt tatataaaaa
     2701 gtaatataaa ttgataaaat tataaattta tttatagtga atataggttg gattttatag
     2761 atacaaaaat agtatatatg atatgtagac cattagcatg tttaaagtag caaaacatat
     2821 aaaataaaat ttgaaaggaa gtataaaaat gacaagacca tcaatatatc atagccaagg
     2881 atacacttgt gcagaggcat taataaagtc ttataatgag gaacacaaca cagatatacc
     2941 aatctcaata ggaagtggta tgggggttgg aatgaatgtt ggaagtgttt gtggagctgt
     3001 aaatgctgct gttgttataa tagggtattt aaatgggcga aatagtaatt tagatgaaaa
     3061 tatagcaagg aagtattcaa gagagctatt gaataaagta agaaataaat acagctcaga
     3121 aatatgtgta tctcttaaaa aaaataaagt aacttgtgaa gaaataataa atttcacata
     3181 tgatgctcta aatgatatgc taaaaaatca aaaataattc taagaaacaa gtattttaca
     3241 ttcgatagaa tacttgtttt tttatgatat ttttaatagg tattagttta ttataatttt
     3301 taaatcaatt ttattatatt ttaattgaaa taatctactt tgaaaattac taactgacaa
     3361 aagaatatga ttatagtata gaaaaaatgt aaattattta atattaagtg agagtatatg
     3421 cttacctata taaaatcgta taaattaatg gctaaattta taaattataa agaaatatat
     3481 aagaaaacaa aagaattaat tagtataata taaagttaaa aagtgaaaat ttatataatt
     3541 tatttataaa gtaaagaaaa aatagaaatt tgtaatgaaa taaaaagttt atgacataat
     3601 atggtataat tagtaaaaat atagagattg gaggaattgc tttgtttata gatgaagaat
     3661 tagaagggta tatattaaca tgtaaaatat ctgaagactt taaaaatata cctgaatata
     3721 gtgatgaaga gttttatgtt acagtctata aagatgaaag ttctgactct gggtactatg
     3781 ctttattaga aaataaagaa gaaagagttg tatgggatgg agaagttgtt gccaataata
     3841 tttttaataa cctttggatt gtagtaaata aggttaaaac tggataatag tctaattata
     3901 tagtatttgt aatattttaa atgaattttt acatatttga gatgagtaaa taacaaagat
     3961 tattcgatac tggaggagta attcaatgga atatagttat agtaagatga acttaaaaaa
     4021 aggggatatt gtagaggtaa atttagagaa acaagccaat gtaatattgt tagaccatat
     4081 aaattatgtg aaatttaaaa atcaaagaaa ttatgattac tatggtggat ttgcaaagaa
     4141 aaatccatgt agaatgagag ttcctaatac tggaacatgg tatctagtag taaatcaaga
     4201 tggaaatagt ggaatagtaa acttttcaat caatacaatt caaaattaat tttagaatat
     4261 tggatattct tactttttgt actttttata tagttaaaat aaaattaata agcaattatt
     4321 ataaaataat gtgggtgcct caaaatgaat tttataattc acgagacact cttttttgta
     4381 taaactcaaa tatactttat catttcaaca atgaaaaaga ggcgtatctg tgttttgaga
     4441 tagcctcatt tatcatatat catatataga attttaatta ttcttaacta aaattttttc
     4501 cataggttat ctgtacaatt aaaatattta ttgttagcat acagctccac ctgtaaggtg
     4561 taaaagagcc tctagtatct cttcttttct ttccaaaact tcatctgata aaagcatttc
     4621 tataaattta tcagcaccaa ttctatcgat agttattcca aaacgttcac ctgtgttacc
     4681 ctgttctcta aataaaagaa ttgctttttc aattaaagtc ataagttcct cttttgaaaa
     4741 taatctatca atctgtgttc caggacgaac tgattttccc catttccctc ctatatagac
     4801 tttataacca gattcttttt cttcaatact atcaaaatta caactttcta tacacttacc
     4861 acagttatta catagattac tgtctatttc aagtttacct ttatctgtta atttagctgc
     4921 tttaacagga cagacctcta caacagcaca ttttttacaa ccaacacaaa gctctgaatc
     4981 atagtcaggt acacgttgac caacaattcc aagatcgttt aggtcaggtt taatacagtt
     5041 attagggcat ccaccaacac caattttgaa cttatgaggt agttttacat cataccaatt
     5101 tttgtaaaat tcttcgtgta tttcagtagc aagtgcctgt gtatcagcta gaccatgaac
     5161 acaaacagta cctttacaag ccacaacagg acgaaccctt gatccagttc cacctgttat
     5221 tagattttcg ctagcaataa attcacttag agcttcaata tcttcaaatt ttacaccagg
     5281 taattctact gttagtctag tagtaaatgc taactgaccg tttccgaatt tttcagcagc
     5341 ttctgcaatt ttttttgctt gtgatgcatt tattacacca tttacagtta ttatacgtgc
     5401 tgcaaaatgt tctccatccc tgcttggtag aaaacctcta ccttttactt cctttttttg
     5461 ttctgctgta acattactca tatttactcc tccattacct taagatttgg ataaaatagt
     5521 attccacctt caagaacttt cgtatttgta tagccaaagc gtcttaagcg attttgcgcc
     5581 atatagctat ttttaccttt agcacatata agaagaattt tttcatcctt agcaagacct
     5641 tcaatttctc cattaatttc accaacagga atataacgta aactaggaat agaaggagct
     5701 tttgaaacat caagtttagt ccaactgtca aaatcctcaa tttcgtcaag taaaacacta
     5761 tcaagttctc cgttaagttt attttgtaaa acatttactg ccactacaaa agggtgaata
     5821 gctgttgaaa aaggtggtgc atatgcaagg tccatacttt ctaatgaata aaggtcagcg
     5881 cctaaagtta ttgcagtagc agcgatatct actattttat caaccgctcc aggtcccata
     5941 acttgaacac ctaataattt tctagtattt ttttcagcaa ccattcttat aataaaatta
     6001 gatgcacctg gataataatg agctttatca tcggtagcta tggttacaga gcaaacatta
     6061 taaccttctt tttcagctat ttctttactt agacctgttt ttcctgcatt gagctcaggt
     6121 aattttacta ctgtcgttcc aagaacacca ttgtaagttt tagattttcc agatatattt
     6181 tgtgcgcaaa ttctaccctc atgatttgca gatgacccca taggtgacca tgtaggttta
     6241 tttgttaaag aattttttac aaaagcacag tctccaacta cgtatatatc tttatcattt
     6301 gtaagcatat attcatctgc aataattgta ttattagggg caaattctaa acctgtgtct
     6361 tttagaaaac ctgtgttagg acggattcca actgacataa ccactaaatc tgttttaata
     6421 gctctatttt tagtacgaag tttttcaact ttattttcac cttcaatacc tacaacctgg
     6481 tcacctgtaa aaatcattat gcctttatca gccatatgat tttcaacgta ttcagcaaaa
     6541 tcagtatcaa atccaggcat aacatgttca gccatatcaa caacacttac acgtataccc
     6601 attgcagaaa gattttcagc aatttcaaga ccaatgaaac ctccaccaat aacaacagct
     6661 ctcttaatac cgttttcaat agaatctctt agttttatag catcatttgg agtacgcata
     6721 aagaatactc caggtaaatc tattccaggt attggtggtt ttattgggtc tgcccctgta
     6781 gcaattacaa gtttgtcata gcttagattt tctttttctc ctgttgaaag cgttttcaaa
     6841 gttacttgtt ttgcatttct atcaatagac gtaacttcaa ctccacaacg aacttctgct
     6901 cctgttagtt tagaaaaact ttcaggtgta tttacgatta aagatgattt atcttcaatt
     6961 atcttaccaa cgtaatatgg aagaccacaa cctgcataag aaatatcatc acctttattt
     7021 agtattgtaa cagagcaatt atcacccatt tcacgtttta tttttgcagc agtttttgtt
     7081 ccagcagcaa ctccacctat aattaatact ttcacaatat atcacactcc aatctacttt
     7141 ataactctat aataaaatta tacttcttga caatatattt gtaaaatact aaaatattaa
     7201 taaaatcata agcatttact aatgtttgct aaggttgtga taatatgaca attcgtagtc
     7261 ttgaaatctt tgttaaggtt gctgaatgtg gtaaaatgag tgaagtagca agaaatatgt
     7321 atattaccca gtcatcagtt agccaagcta tttcagaaat agaaaaagag tatggtgtta
     7381 agttatttga tagaatttca aaaaaattat atttaactga ggcagggaaa aaacttcttg
     7441 gatatgggag gcatctttta gctgtaaatg aagaaatgaa tgattgtatg aagcattgtg
     7501 caaaaaatat ccgtataaga gttggggcaa cagtaactgt tggtacatgt gtaataagcc
     7561 cgataatgtt agaattatac aaggtaaatc ctcttataga acctgaagta tttgtagaag
     7621 atacaagact gattgcaaaa aaattactta atagtgaatt agatattgcg attgtagaag
     7681 gaaaaattaa gcacccagat attgttacaa aaagcattat taatgataat cttgtactta
     7741 tatgttcaca caagcatgag ttctacaaaa gagactctat aaaagtttca gaactttcaa
     7801 atcaaccatt aattatgcgt gaattaggaa gtggtacaag agcacaatta gaaaagcaat
     7861 taaaagaatt aaaaatccca atgaatatta gatggtcatg ttataactct gaagctatat
     7921 taagagcagt tgtagataat tttggcattg cagttatatc cgaattactt attgaggatt
     7981 atttaaaaaa gcatcttttg tgggcttgtg atattgaagg tattaactta catagaacat
     8041 tcgatattgc atatcataga agcaagtttt ttacagaaaa tatttcagca ttttttgata
     8101 tttcagtaga atatgggaaa aaacaagcga gaaagaagag tagaagtgta aacagtttat
     8161 aatatatcta aataaaaata agagtatatc aatatactct tatttttatt tagatatatt
     8221 agcctatatt aacgccaaaa tttttacaca atcctgcaag tccatcctca aatccagaac
     8281 ctaaagcatt aaatttccac tctccattat gtctatatag ttctgccacg actattgctg
     8341 tttctatgct aaaatcttca cccaattcat atttaataag ttcctcgtta gtttcttcat
     8401 tatatattct tatatatgaa ttttcaactt gtccgaaatt ttgtcttctt gtaattgctt
     8461 catttatagt aacactaaat gatattttag caatattttg aggtatttta gataagtcta
     8521 catttatttg ttcatcatca ccatcaccaa cacctgttct attatcaccc atatattcaa
     8581 cagctccaga agcgtgtttt aaattattat agaatataaa gtctccatca ttcgttactt
     8641 ttccatcagt tccagttaaa aatgcagagg catctaaatc gaaatcaaat ccaccatcat
     8701 atttattaat atcccatcct agtcctacta ttactttttt tagaccaggg ttactttttg
     8761 ttaaacttac tttttgtcct tttgctaaag ttatacccat ttaaaccaac tcctcattat
     8821 aaatttttta ttgtaattat tacttatatg tattaattaa ttcacgaagc tcttcatttt
     8881 gaccaattcc taaagcatta aatttccatt cttcatttct acgataaatt tctcctgcca
     8941 aaactgttac tttaccagaa aagtcttctt gaagattata tttaaataat tcttgcttgg
     9001 aatttgaatc aattaatctt ataaaagagt tgttcatttg accaaaatct tggtctcttt
     9061 ttttagcttt gtatatagaa actccaaaaa ctattttatg tacatattca ggtatgtctt
     9121 ctaaattaac aattatttgt tcatcatcac cttcaccttt tccagttaag ttatctccag
     9181 tgtgtataat agctttagaa gggtgtttaa gatttccaaa ataaaccaag tcacctctaa
     9241 cattcatttt atcttggtta gtcaatgcta ttgcaaatgc atctaggtca taattttttt
     9301 tacccaattt attcatatcc catcctaagc ctactactag attttttaca ggattattat
     9361 cttttactaa gctaactctt tgtccttttt gtaaactaac actcatttta agttctcctt
     9421 tttatatttt tagttttatt gatggtatca agtataagaa ccatattagc tatattatat
     9481 acgtatttag gctaagttgg ctatatttat aaattattta ttatataact acatgaatat
     9541 tttataattt aaacatattg acattatatg atttaaatta ttttgtagtt ttataatatt
     9601 taaatttgta tttgattgtt ataaagtaaa gtaagtaata aagttagact caataattct
     9661 aaaatttcta agcacactat ctaaatgaga acgtatttgg aaatacttgt ataaaacaat
     9721 aattgataca tttaattttt atcttaaaaa taacaattat aatatataaa tagaaagtat
     9781 aatatataaa tagcaagtat aatataacat ataaaaagtt gaatttgtat atgttaatta
     9841 tacttgctat ttattgttag ttattctatt tttttaaatc cattacctaa agcttcatgt
     9901 atatctgtaa ttataataaa agcattaggg tcgacatttt ttactaattt ttttaattcg
     9961 acaacttctt tcttttcaac tactactaaa agtacatttt tattttctcc agtatatgca
    10021 ccttctcctt ttagaatagt tgcacctcta tctaaatctt ccattatttt tttacttata
    10081 tcttttgaaa ggtcagatat aatagtgaat gatttggaat aattaaatcc ttcaataagc
    10141 aagtcactaa cttttactaa aatgtataaa gccacagttg aataaagtgc aatttcaacc
    10201 tgtctattta ctatacctga taaaacaact acaacgcagt ctacaatacc cattaaaaca
    10261 ggtatactaa atgtaggaaa atatttattt agtaggagtg ccattaaatc agttccacct
    10321 gtagagccat ttattctaaa aataagtcct tgaccaactc ccattaaaat agaaccagat
    10381 ataattgcaa gatacatatc atttgttata tctaatgtag ccatatttgc agtaagctta
    10441 agtgctatgg taagaaaaat tattcctaaa accgttttaa aacaatcttt ttttgaaagt
    10501 attttgtaag caaatatgaa taatggaaca tttaaaagta aattgacaat ccaaagttct
    10561 acaccgaata atgaatttaa aacaactcca agtcctgtta taccaccagc agcgattgca
    10621 tgtggattaa aaaataaatt aagagaaatg gccattaata tacagcctac aattaatatt
    10681 aaaatttctg aaaaagttat tctatctttt ttcaataaca tcactcctta tatatagatt
    10741 ataatatata ttatatagtt tttccaattt ttaataaaag aaataaaaat atattataaa
    10801 aacaatttga ttggaatttt cagaaaatta atgctataat taaaagtaaa tttaaaggga
    10861 ggttataaaa tggctaaatt aactctagaa catataaaag gaaatacata ttatattcca
    10921 ctccctacaa tagtaggtgt atacgtagat ggtaaagatg caattttaat tgacagtgga
    10981 aataataagg atacagctag acaagtttta agattattag aagaacataa tttaagtcct
    11041 aaattaataa taaacactca ttcaaatgca gaccatatag gaggaaatgc atatttgaag
    11101 aatcaaacaa aatgcaagat tgctacgact aaaattgaag gatattttac agaaaatcca
    11161 atattagaat cagcattttt atatggtggc tatccaagta aagcactgaa gaataaattt
    11221 ttattagcaa aggaaagtga agttgattat attatacctt caaatggcaa aattgtagat
    11281 acagaacttg aagcgatttc tcttccaggt cattattttg aaatgattgg tgttagaact
    11341 ccagataatg ttttgtttgt tgcagatagt cttacaccag agaatataat tactaaatat
    11401 cattttttct tcctactgga tatagattca cagtttaaga cactagaaaa attgagaatg
    11461 ttagaggctg atttctttgt tccaagtcac tctgtcaaaa ctacagatat aaaaagtttg
    11521 attaatatta ataagaagaa gatggaagaa ataatagata atattaaaaa ggtttgttat
    11581 gagccagtta tgattgatga agttattgaa aaaatgtgtg atttgtacaa tgtcaagctt
    11641 gatgctaatc aatatgtgtt ggttggaagt acaatacgtt cttatataac ttatttatat
    11701 gagaataata tggttgaata tatatttgat ggtggtaaga tgatgataaa agctgtgtaa
    11761 atagtaataa ttttcaaatt atatgatgaa attgttttag tatgactgtt atttgaaaag
    11821 taacataaaa aaatttatat aaataagcta agatgattga tataaagtta ctgataattt
    11881 taaggtaata tatacgttac tataatatta tgttattatt tgtaaataag gggataattt
    11941 ataagcaaat taaagacctt aatttacagg aggaatagtg tatattatga ataaaaaatt
    12001 caaaaaattc agtaaaaaac taagtatatt aattttagtt ttgactatgt ttctttttaa
    12061 taatttaact gcaaatgcac agattaaaga agattcaata ataggaattg atagatatga
    12121 aacagcagga cttatagcag atagacaaac ttatgatact gtaatattag ttaatggaga
    12181 taaaaattta tctgatgggc tttcatcaag tggattagct ggagctatta acgcaccaat
    12241 acttttgact aaaaaaagtg aaataccaaa agctacttta aatagactag ataataagag
    12301 tttaaatgtt gtaaaaaaag tatatataat tggtggttat aatactattg aaaattcagt
    12361 agaaaaagat ataaaaagaa aaggtattca agtggaaaga ataaatggta acaatagaat
    12421 tgaaactagt tataatgtag ctaaaaaaat aaatgaagta agtagtgtaa aagaggtaat
    12481 gcttacaaat ggatttgtag gagaggctga tgctatgagt atagcacctg tagttgcaaa
    12541 aaataaagga gctataatac ttacagatgg gaaatcagtt ccatttaaga ctgaaggttt
    12601 aaatgtttac gctataggtg gcaaatctgc gataaatgag gatttagtaa atgaaactaa
    12661 agcaactaga atcggaggaa atgatagatt tgagaccaat aaaaaggtaa tggaaaagtt
    12721 ttataatgga gcgacagatt tttatataac aaatggatat caattagtag atgcactaac
    12781 attatctcct ttagccaaag aaaaaccaat cgttttagtt gctgatggaa gtaataaagg
    12841 tattttaaaa ggagctaaaa gtataacaaa agttggaggt attgatgcca atatatataa
    12901 acagtgttta gatatgatta aatataataa tatggatgtg actccaaata ttttaaaaca
    12961 cttaacttca gttgaaggaa atgaagtttc tgaaatactt atagaatcag ataatttagg
    13021 tgtggttgtt gaaaagacga ataccaataa atttgagttt gattacgtaa gtgtaactaa
    13081 tgaaaaaaat tgtacttttt ctgtatataa agaaaattct tctaataatg taaaaaatgg
    13141 aaagctagtt gtaagtgcta agaaaaaaat agataagaaa gatagtgatt tgcaagatat
    13201 gaatggcgac aatatgataa atgcaaataa agataggatg gtaaatgtga taaaaattgg
    13261 agttcccgat aaaatgtatt ctaattttaa agtagatgtt gaaagtggtt cagtagaact
    13321 ttataatatt aaaggaggag ctactgttga tgtcaatgac ggaattgcta acattgttga
    13381 taacaatgtg acaaatcctt tcaatataaa tactaatagt gggatttcag gtgtaacagc
    13441 tgaaactata tcatctgaga taaaatttag agcaaacaat ggaattgtaa gtgtaacagc
    13501 tacaaatata aatggcaata taagtttatt tggaagcgat aataaaggta cctttgatgg
    13561 aatatttaaa ttaaatctga aaaaagaacc atcaaattta cacttgaagt tagttggaaa
    13621 tgggataaat aaattgccag aaggttggtc taaagattat attttaggaa atggacaacc
    13681 aattattgaa gttaaaaata atggcataaa taatatatca attggagaat aaaaacatat
    13741 gaaaataaag tttatatagt tttaaaaatt atagaatttt aaaatattta tatatattct
    13801 ttaaataaaa aattattttt taagtgataa ttaagtattt atcgataagg ctatggttgt
    13861 aaaaatcata gccttatttt ta
//
LOCUS       DAGQWR010000109        13847 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.109, whole genome shotgun sequence.
ACCESSION   DAGQWR010000109 DAGQWR010000000
VERSION     DAGQWR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.109"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            575..2005
                     /locus_tag="MIW95_002816"
     CDS             575..2005
                     /locus_tag="MIW95_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3152128.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            2108..3211
                     /locus_tag="MIW95_002817"
     CDS             2108..3211
                     /locus_tag="MIW95_002817"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBY3152129.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMTYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            3226..3462
                     /locus_tag="MIW95_002818"
     CDS             3226..3462
                     /locus_tag="MIW95_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY3152130.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEKACQEA"
     gene            3479..4528
                     /gene="vorB"
                     /locus_tag="MIW95_002819"
     CDS             3479..4528
                     /gene="vorB"
                     /locus_tag="MIW95_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBY3152131.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4530..5270
                     /locus_tag="MIW95_002820"
     CDS             4530..5270
                     /locus_tag="MIW95_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBY3152132.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            5273..5812
                     /locus_tag="MIW95_002821"
     CDS             5273..5812
                     /locus_tag="MIW95_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBY3152133.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLLEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5936..8035
                     /locus_tag="MIW95_002822"
     CDS             5936..8035
                     /locus_tag="MIW95_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBY3152134.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKMIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWALDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNTKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8302..8817)
                     /locus_tag="MIW95_002823"
     CDS             complement(8302..8817)
                     /locus_tag="MIW95_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBY3152135.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8909..10120)
                     /locus_tag="MIW95_002824"
     CDS             complement(8909..10120)
                     /locus_tag="MIW95_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBY3152136.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDFPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            10796..12238
                     /locus_tag="MIW95_002825"
     CDS             10796..12238
                     /locus_tag="MIW95_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBY3152137.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            12280..12633
                     /locus_tag="MIW95_002826"
     CDS             12280..12633
                     /locus_tag="MIW95_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152138.1"
                     /translation="MKKSNIFSIIFPTIIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            12824..13645
                     /locus_tag="MIW95_002827"
     CDS             12824..13645
                     /locus_tag="MIW95_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBY3152139.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIKKVKSNNIA"
BASE COUNT         5291 a         1599 c         2463 g         4494 t
ORIGIN      
        1 tgttttattg gttttagatt aatatagcta taataattta tgatattaga tggaaattac
       61 gtaaaaatag aaaattgaaa attctgaaat ttttgcatat tgatatttat ctcatagatt
      121 cttttttgta attgtaacac gcttttattt ttaaatcaat agtacatgta taaaaataaa
      181 aggaatctaa ataagaaaaa gatattttta atatctggat gtagtataga aatatcttga
      241 ataaattatt cttgaataaa attataaaaa tttaaaatca ctagaaatat gataaaataa
      301 tttaaaatac gcatttcaat gacatttcaa gacaagaaaa taataaaaaa caaatctaaa
      361 ttaagcctaa atagcttttt agttaatttt agtagtaaaa aataaaactt aaaaagtgtt
      421 tgacaattat gaattttaag aatataatta aattaaaatt atacacatat aaaaatatac
      481 tcctgaatca agaatgagat atttaagatt caaaatatat attattatac gtgaataatg
      541 aaaagtaaga aaattttata agggaggggt agatgtgagt agtcaagaaa gcaaaccaaa
      601 aggaaatctt atatcaaatt ttagagatga tatgagagac cctaagaaaa gagcaatata
      661 cacatattca agttctataa acttagcgta caattttata ggttactttg tatcaactgt
      721 tacaaatatt tttttaactg atgtggcaaa acttggtgta attatggcag gtggtatgca
      781 aattatacaa tctttcataa aaattgctgt agctcctata acaggtacag tatttgacaa
      841 acaaccattc aaaaaaggaa agtattatcc atggcttaga tatgcaccag cagcgttagc
      901 attaacatat ggaatatttt tcatagttcc attaatgaaa ataagtccag cattactttt
      961 acctttgatg acagctttat tattaatagc atcttgtgct cagcaagtag ttttcactat
     1021 aatttatgct atttaccctg tagttgctaa gacacctaga gaccgagtta taggctcaac
     1081 tactactaat atatttaaag aagttggtaa attcttagta ggtttttcat atccgttatt
     1141 attagtttta tttgcagatg tattaggtgg agaaggaatg ggttatttag gtacctactt
     1201 attctttgca gcaggatgcc ttttagtatt ttggttttct tcaacagaga taataaaatc
     1261 tggagcagaa gaagaagttc ttcaaaaaga agagaagcca aagattaaag caagtgaaat
     1321 ggcaaaagct ctattcacta atgtaccact tatgatggca ttttgtttag aatttcttat
     1381 atgtataaga tctataggat taggaccttt ggctccatat tattttaaat atgtaataga
     1441 agatgaacgt ggattagcag tatttttatc aatcatgcct ttggtaagtg tagcattcat
     1501 gtttttcgca ccagtgttta taaaaatctg tagagagcaa aaactagctt caataatttc
     1561 tttttctata tgtgcactat gccacttatt ggttgcatgg actccttggg ggaaaactac
     1621 tttaggagta actatgttgc tggcaattgg tggaggtttt tcaaatgtag tatcaataat
     1681 aaatcttaat ttctttgccg gttcttgtga ttatgggcat tggaaatcag gaaaagactt
     1741 acctggctta agtatgtcac tatatccagt tgcaatacaa gtaggagtat tacttgcaac
     1801 tactataaga acagttttaa tgaactcaat gggatatcaa gctgatatgg tagtcacaga
     1861 agcagttaaa tcaggtttta taaatatgat ttcttattct atggcaatac ctttaataat
     1921 agctgttgtt atagcaatac tatatccagt aagtgacaag aaattaaacc aaataagaga
     1981 agagttaaat caaagaaatg cttagttaaa agaaagttaa gtagatggtt aatttaagct
     2041 attaaagtaa gtagacgcaa tagcgtttat ataaatcaaa atacatatac agagggggct
     2101 tttaaaaatg atagaaaaat tattgagaaa taccgtaaaa gaattacatc aatatgttcc
     2161 aggtgaacca atagaaaaag ttaaggaaaa atatggtgta aaagaaataa taaaattagc
     2221 ttcaaatgaa aatcctctag gaccatcacc aaaagcaata gaagctatga ttgaaatgct
     2281 aaaacaaggt caactttatc cagagccaga agctaacgaa cttagaagaa aattagctga
     2341 aaagctagat ttaaaaccag aaaattttat tgttgctaat ggtgcggata acgttatcac
     2401 tctaataggt gaggctttta taaatagagg agatgaagtt atatattgta acccaacatt
     2461 tccatcatat agaactgcaa ctataaaaaa tgaagggata ccagtagaag tgcctttaac
     2521 agaagattat aagtatgatt tacaaggtat attagataag attacagata aaacaaaatt
     2581 aatatgtgta tgtaacccta ataatccaac aggaacaatt gtggatgata aagagttaga
     2641 agaattttta aagaaagtcc ctgaaaatat tataactata ttggatgaag catatataga
     2701 atttttaaca gttcctaatt atgtggatgg acttaagtat gtaagggaaa attataacgt
     2761 tatagtaaca agaactttct ctaaaatata tggtcttgca gggcttagag taggatatgg
     2821 tattgctaaa gatgagataa taagaacttt atttacagta aaagagcctt tctctgcaaa
     2881 tcgtgttgct ataagtggag caacagcagc acttgatgat gaagagttta taaaagaaac
     2941 atatgaatta aatagagttg gaatgactta ctttaaagaa gagtttacaa aaatggggtt
     3001 tgatgttgta gattcacaat caaatttcct ttatgtagat atgaaaacag atatacctaa
     3061 gttatttgaa gacttgaaaa aaagagggtt tgtaataaga ccaagtgcta cacatgcacg
     3121 tgtaagtatt ggaactatgg aagaaaataa gaaatttgta gcagttttaa aggaaatatt
     3181 aaatattaaa ggagagccaa gtattggata atagatagaa atattatgaa acggggtgta
     3241 gatatgaact taaaaataaa cgcagagaga tgtaaaagtt gtgaatactg cgttatatct
     3301 tgtaaaaaag gagcattaaa aatatcaagt aagataaata aagaaggtta tgctcatgta
     3361 gaagttgatg agagtaaatg catactttgt ggaatttgct atcaggtgtg tccagataat
     3421 gtttttgaaa taattaaaga aaaagcatgt caagaggctt agaaggagtt gggataatat
     3481 gaaaaagatg tggaagggga accacgccat tgcagaagca gccctaagag gaggttgtga
     3541 attttatgca ggatatccaa ttacaccaca aacagaggta atggaatttt tatcacatag
     3601 aatgtctgaa cttggaagaa cttttataca gtccgaaaat gagatggctg ctatatatat
     3661 ggtatatgga gcatatgcaa gtggaatgag gtctatgacg agttcttcag gaccaggaat
     3721 atctttaaaa caagaaggga tttcttactt atgtgcaaat cattaccctt gtgtaatcgt
     3781 aaatgtacag agatgggggc ctggtcttgg ctcattggac tcagcacaga cagactatct
     3841 aagagatact agagggggag gaaatggaga ttatcatttg atagtttatg caccaaacag
     3901 tattcaagag actgtagatt tgatgtataa ctcatttgat gtagcggaaa aatacagagt
     3961 gcctgtcgaa attttgacag aagcagcttt aggtcaaatg atggaaccag tagaatttcc
     4021 agaatttaag aagagagaag gagatttagg ttggacttat gatggaagta atagagacca
     4081 tgcaaaggtt gcagataacc aaaagccaac tttttgtatg gagaaacgta tgagaataac
     4141 tgaaaatgaa caacaatggg aagattatca agtagaagat gcagaatatg tgtttgtagc
     4201 gttcggtata ccatcaagaa caactatgaa tgctgttaga agacttagag aacaaggtga
     4261 aaaagttgga ataataagac caataacagt atggcctttc ccatataaag catttgaaaa
     4321 ggtaagtaaa gatgtaaaag gatttatctc agtagaatca acagacacag gtcaattagt
     4381 agaagatgtt gctttagcct caaagaaagt atgtaaagaa aatgtacctg tgtatggttt
     4441 atttagtgga aatcatatac caaaaacatt gcaagtaatg gacacttata gcaaaataaa
     4501 atctggcgaa atcaaggagg tgttctaata tggctacaga aaaattaaca cctagcgttg
     4561 tttcaatacc aaacaagttt tgcccaggtt gtggtcatgg aatagtaaat cgtataatag
     4621 cagaggtaat agaagaacat ggatatgaaa aaaatcatgt attaacatta ggagttggtt
     4681 gtgtctgcaa tatgaatttt agctggaatg gtgataagat gcaaactgca catggtagag
     4741 cttcatctac agctatagga gttaaagtag cacttccaga tacattagta atgacttacc
     4801 aaggtgatgg agatgcttat gttataggtc tttctgaaac tttaaataca gcatatagaa
     4861 atcataatgt gactgtattt gtaataaaca ataataattt tgcaatgact ggtggacaaa
     4921 tgtcatggac cactatgccg ggtcaagtca caacaacaag tgtaaatgga agagatataa
     4981 agactactgg tagtccactg aaagttcctg agatggtggc aaacttcttt gatgtagcat
     5041 atgtagcaag aggttcagtt catagcccaa aggaaataat taatctaaaa aaatatataa
     5101 aaaatgcaat agaagctcaa ttaaatggag agggatattc cttagttgaa atattagcac
     5161 cttgcccaac taactggggt gtatcactag agaaatctat caaatggatg gaagaagaaa
     5221 tagtgccata ttatgctctt ggcgaattta agcaaaggga tggtgaatag ttatggaaaa
     5281 agagctagta tttgcagggt ttggtggtca aggagtttta actcttggtc tgattgtagc
     5341 tgagtcagca cttgaattgg ggaaacaagt aacttggatg cctgcatatg gtccaactat
     5401 gagaggtgga aaagcctata gtgtagtaaa gttttctgat gagagtatag gtgggcctga
     5461 tatggaagaa atagatgttt tagtagcaat gaataaacca tctttagatt atataaattt
     5521 agttaaagaa ggtggaacag tagttattaa tacatctgca atagatgaga atgtaacatt
     5581 aagagaagat ataagtgtag ttaaaataaa ttgtcaggaa ttagctcaaa aagtaaacaa
     5641 tccaaaagca gcaaatatag tagtacttgg agcattaatt gctaaaactg ggttacttga
     5701 aaaagaatta gctttaaaaa ctatgtgtga tttctttgag gaaaaaggaa aaggaaagtt
     5761 taatgttcaa aatgaagcag cattcatgga aggctataat aacgccattt gataaattac
     5821 ccttatctta agtgatttat ctcaagattt aataatcttt agtgccaaga gaaacttcat
     5881 cttgagataa aattcactta aaatcacaac tataaacata taggagcgtg aatttatgag
     5941 tttggaaaaa cttattagac caaaaagtat tgctatagta ggagtgactg ataaacttgg
     6001 ttttggaaga agtgcagcac ttagtatagt aaaaagtaag gaaacagata gagtatatta
     6061 tgttaatccc aaaagagaag aattgtttgg gagaaaatgt tataagatga tacaagaagt
     6121 tccagaagta gtagactgtg tagtagtatg tacgcctaga aatgtagttc catctgtatt
     6181 aaaagattct ggagagttag gaacaaaggc agcagtggta tatgctagtg gatttgcaga
     6241 ggaaggtaca gaggaaggta cagatttaga aaatcaactt atagaaatat caaatacata
     6301 cgatatgaaa atattgggtc caaactgcat ggggttgcta aattgtatag ataaggttaa
     6361 tctatgggca ggaggttcta aatgggcttt agatactaaa aaacctggta taggaattgt
     6421 aggtcaaagt ggatttataa ctgctgaaat tgtttcaagt gattatttta atatatctta
     6481 tggtttttca acaggaaatg gaaatatagt tacactagaa gatgcagtag atttccttgt
     6541 agatgatgat tatgcatcag taatagcaat ttaccttgaa ggtttaaaaa atcctcaaaa
     6601 atttatagat gctttaaaaa gggcagcaca aaaaagaaag ccagtaataa tattgaaatc
     6661 aggaaggtca gaaaaaggtg ctatttctgc tgcctctcat acaggaaatt tagctggttc
     6721 gagtaaagct tttgaaagta tttttgaaaa atatggagta atttctgtgg aaaatttaga
     6781 acagtttatg tgtttagcac aagcttttag tgttttagat ggtaatttac ctgcaaattc
     6841 taattttgca gcaataaact tctcaggagg agaaaatact atttgtgcag atttagcaga
     6901 agaaaatggt gtagaacttg cagaaatatc ttctgaaact aaagaagaaa tgaaaaaata
     6961 tcttccagga tttgcaacac ctaaaaatcc actagatgct acaacagcat tattccatga
     7021 aaaggatatg atagtaggtt tattacatac ctttgaaaag gatgatagtg ttggatttac
     7081 tatggtaggt gcaaatataa gagatgaaga aaatgaaatg catgaaaccc tttgtaaagc
     7141 tgtaagtgaa gctagagaac aaggattgaa aaaacctgtg tttgcagttc ctacacttga
     7201 aggaaataga tatttagatt ataggactag attagaagat aatcaagtgc ctataatgtc
     7261 atcagttgga actacattta cttgttttaa taaaatggct aagtttatag attatgatta
     7321 ttcaaaaaga actttagaat ttaaagctgt taagaaaaga gagtcaaata atgtagtagc
     7381 attatcagaa ttagatagta agattgagat gaaaaaatat ggagtaccag ttccaggaca
     7441 aggtaataca aaaagtattg atgaattaga tgaaatctta aaatatatta agtatcctgt
     7501 tgttttaaaa ataaattctt ctgaaatatt gcacaaatct gatgttggag gagttaaaat
     7561 aggaattaag aatagagatg aggcagtaga tgcatataat gagattttaa ctaatgtaaa
     7621 aaaagcaaaa ccagatgcta atattgatgg tatattagtt caagaaatgg tagaaagtgg
     7681 tatagaaatt attattggta taactaatga tgaccaattt ggaccaatgt tattagtcgg
     7741 tttaggagga gtatttgtag aggtatttaa agatactaca ttatatccat tacctataaa
     7801 tcatgatgaa gctattatga tgcttaaaaa actaaaatca tttaagcttt taaatggcta
     7861 tagaggaagt gagccttgtg atattgatgc tcttgcagat atgatggtca aattaggaaa
     7921 atatgcttat gaaaataagg atgaagtcaa ggaaatagac ttaaatccag tgtttgtata
     7981 tcctaaagga aaaggcgtat gtgcagtaga tgccttaata gttaaatata aatagtttat
     8041 aagttttggt attcaaccat aattaaagca ccgactgaaa atcacatttt gtaagttaga
     8101 tagtgtgatt ttcagtcacc ccaaatttaa tttaaaaata ttttaaaatg aaagtatagt
     8161 tttgagttta attgtgaaat agcactgtat aaacaataaa atactacgta taaaacgaat
     8221 aataaaaggt gttttaatga taagaatcaa tcttgatttt cattaaaaca ccttctttat
     8281 tatattaaaa atttttaaac gttatattgt cacaggtggg tttgttggag ctgcatatgt
     8341 acgattagca aattcttggt caacaaagaa tagaccaccc tctccagcaa gtttaacacg
     8401 ttttatattg tcttcacaat ttgtttcttc ttctgcttgt tcagatataa accaaagtaa
     8461 gaattgagaa gttctaaagt ctgattcaga gtttgctaca gctgctaagt tgtttattaa
     8521 agctgtaacc tttttttcat gagctaacgt acgctcaaga atatccattg cagaagtaaa
     8581 gtctgaatct ggcattggga tagcatcaag ctgaaccttt cctcctacat tatgtaagta
     8641 tttgtaaaaa aacatagcgt ggtctgtctc ttctttatat tgaacataaa accaattaga
     8701 aaatccttta agtccttctg cttcaaggta agaagacata gaaagataaa gatacgcaga
     8761 atataattca tggttaatct gctcattaag taatttttcc attttagctg aaatcatttt
     8821 tatacctcca aattttaaat gctcttataa aatataataa tattaaatgg aaaaatattc
     8881 aataaaagtg tttaaatatt ttacatattt aataaaaatt tatactacca ttaaaaaaat
     8941 tataacttac aatggctata gagccaatta atggtgcatt tgctccaaaa tttgaatgcc
     9001 ttacacttat ttttttatat ttagaaaaac ttgctgaagc agtcaatttt tttaataatg
     9061 tattttctat aaatgtacca ggtatattaa agtcatagcc tattattata gaactacaat
     9121 caatcaatgt aagtgtatta actaatgcat atgatacgta tgtacaaaat tcatcaagca
     9181 aaaacatggc aagactatct ttattatttg cctcatctat taacttgact aaagaaaaat
     9241 ctttacagtt aattaatggg gagttaggat aaatatggct caatgattca attcttttaa
     9301 ttagattact gacacttgta taataatcta aacatccatt gttaccacaa ctgcacaatg
     9361 gtccactaaa attaattgat gtatgtccta tttctccact ttgtcctagg ttgccagtat
     9421 gaagtttatt ttctagaact agaccagctc caataccatt cattatgtgt aggtatatat
     9481 aatttgatat atttttacct aaaccataca ttttttctgc taatgcaccc gaattagcat
     9541 catttatcaa aaatattgga aaatcagaca tttcttttat aaaatctact atatttaggt
     9601 ttgatacatt tccaaagtct gggggattta gtatggtacc atttatatca tctactggac
     9661 ctattgaaga aataccaatt ccaattatat ctctcttgtt gattttcatt attttagtaa
     9721 acattctttt tattatatct tttaaaacat catttgatag acatttaggg taagtttcag
     9781 aaagttgttt tacaatatta ccttttaaat ctgaaataat taccttacat atatttcttc
     9841 ttattaggat tccacaaata caaggcgata tattagaaat atctaataaa ataggtttac
     9901 gacctgatga atgtgcattt aaagttgatg actctatttc cctaatcata ttttcattaa
     9961 taagttcagt aactagattt gataaagtca ttttagtaag tcctgtagac ttagctaaat
    10021 caactctgga cataggtgta tttgtagaaa gtaatttcaa tattattaac ttatttttaa
    10081 tttttacatc tttgatgtta gaaccttgtt tgttactcat cataaatctc cttaaataaa
    10141 gttaagtgtt ttatttattt tcaagtatac tatcacctta taatagagta acattttttt
    10201 acttgaaaat atcattaatt agaatatatc acattatata tcataaaaca tataaaaaaa
    10261 tgttagaatt aaattaaaag tgaaaagtag attgatatat aaatagtaaa tacttaatta
    10321 aaaaaagata atgatagcaa taattattgt taccaagaat ctactacttc aagtgttacc
    10381 ttaggtattt aagttgatag tggtttgcac gtaataaact tgatgtaata tatattctta
    10441 ataatatcga taaaagattt acttaaaaat aatatagatt atgtaaacat ataatctatt
    10501 tattgtatac aataaaattt cattttgtaa aacagtgaat taaatatgta atatagatat
    10561 cgatttttat tacaaattgt tactaaatat aggtacatta gaacaaaaat ctagtaaatt
    10621 gtaactaaaa tgtaaatttt tcctttaatc tatacaatat tatggaaact ttttgtaatt
    10681 tatgatataa taacaaatat ataattatta aattataata aatgactata gcgtagatgt
    10741 aatactatgt tatagtcatt tttgaattaa acttgatata tcaaggggga agaatatgag
    10801 aacaattaga agagttttag cattaggttt aacattagct atatttctaa taaatgtacc
    10861 taatgtagat gcattaacat cagatacaat taaaggtaac aatatatatg agacggctgg
    10921 attaatagca gataaaaaga gttatgatac agcaattatg gtaaatatgg ataattctat
    10981 agcagatggt ctttcagcaa gtggtcttgc aggtgctgtt gatgctccaa ttttgcttgc
    11041 acaaaaaaat aagataccaa atgaaacaaa acaaagatta aaaaatgtta aaaagatata
    11101 cataataggt aaagaattat caataagtaa gtcagtagaa actgaactaa aaaacacagg
    11161 ggcacaggta actagattgg gtggagatga cagaataaag actagttata gtgttgctaa
    11221 agaggtaaat ggtatcaaaa aagttgatga agtgatatta actaatgcat ataaaggaga
    11281 agcagataca ataagtgctg cacccgtatc agtaagagat atagctccta tagtacttac
    11341 agatggaaag agtgtgcctt tttcaacaag caatgtaaaa acatatgcag taggtgggag
    11401 tatatcaatg agcactagtt tagttaataa aactaatgct aaaagacttg gtggttcaga
    11461 tagatatgat acaaataaga aagttataaa agagttttat ccagatgcat cagaatttta
    11521 tttgagtgat ggatatgatt tagtaaatgc acttacaggt tctacaattg ctaaggaaaa
    11581 tccaattgta ttggtgtcag aaagtagtga taagtctata ttagcaggag cagataagat
    11641 tactagatta ggttcaataa gtgacagtgt gtataataag tgtgtttctg ctgcacaaaa
    11701 taatggcgat tcgtctacaa aaggtgagtc acctatgaaa aatgagacaa gcatattagg
    11761 tcagccaaca gctagtttag aagcgtgctt aaaatgggca aaatctaaaa aagctaatga
    11821 tttatttata gagttaatac caatattata tgatactgct gttcaggaag gtgttaatcc
    11881 tgttttggca gtagctcaat ctgcaaaaga aactggtttt tgtaattttg gtggagtatt
    11941 agatgcatca tttaagaatc cttgtggact taaaacttct gtaggtggtt ctgatactga
    12001 taagaatgcc cattcaagat ttgatacttg ggaagaagga atattagctc aaattcaaca
    12061 tttatgctta tatgcaggac aagatggtta cccactttca aatccagtag accctagaca
    12121 tgaaaaatct ttatttggta aggctaaaac agttgaaagt ttatctaata attgggctgg
    12181 aggtcaatat ggacaagatt tagtaagaat gatgggagaa attgaggcaa caaaataatt
    12241 gaagattatc aaatatccaa taaataagga ttgattatta tgaaaaagag taatattttt
    12301 agtatcatat ttcctacaat cataatggtg ctaatgtttt taacttttgc tacagatata
    12361 tttaatattc cagatattca atctaaaagt atttttgttg tgggaatggt tttgattttt
    12421 ccaatttctt ttttaataca agggataatt tgtgtattaa ataagactaa ttggatttta
    12481 tctcttattg tgtctttagt aacttatata gtattaatgt atatattttt aaatgattct
    12541 gcatatatat atgttttact ttatacagct ttttatatga ttggacatat aacagctaaa
    12601 ctctattaca aaattaagac ttttaaaaat taaattgaag tatataaaag taacattaag
    12661 agaagtatcc ttaatgattt tctaaaatca gaaataggtg cttctctttt ttgtaaaaat
    12721 aaaagtatgt aaataaaatt taaccattat aattttcttt taacaaatgt atacaataaa
    12781 cttgttgtat aattaagata gataaaataa agggagggat aatatgaatt taatagataa
    12841 tcatattcat actaattttt caagtgatgg aaaggattcc atggaagata caataaaaaa
    12901 agctataagt attggtgtta gatatttgac ttttacagac catttagaac atgatgaaga
    12961 tagaggattt tgcataaatt ataatgacta tgttccagta tttaataagt ttaaagaaaa
    13021 atataaaaag gatatagagt tattactagg tgttgaagta ggttacagaa aacatctaaa
    13081 aaatgaaata gaagaaataa taaattctaa tccttttgat tttgtattgt gctcaactca
    13141 tacaattgac aatgtacctg ttccatcaaa ggcgtatttt aaaggtctta gcaaagaaga
    13201 tgcgtattat aaatatttta atagcatact tgaaactaat cgtgaatttg gagattacaa
    13261 tatatatggt catttggatt atatatctag gtatggaata tattcagata atagagttat
    13321 atacaatgac tttaaagatg ttattgatga ggtcttaaag tcaataatca ataatgggag
    13381 tggtatagag ctaaatactt ctggttatag atatggatta aatgctatac atccaaatga
    13441 agatattcta aaaagatata aagaacttgg aggatttata gttactgtag gttctgattc
    13501 tcatagggta gaagatatat gtaaagattt tgatgtggcc tatgatatgc ttaagtatct
    13561 tgactttaaa tatgtatctt tatttaaaga aagagaaact tattttgtaa atataaaaaa
    13621 agttaaatct aacaatatag cttagagtat gttttgaagt aaattaatgg atagttaaaa
    13681 agttaatagt tataatattg tatcctctag aaaaatacta gaggttttta ttttgctaaa
    13741 ataactaaat agaattaaat ttaaatagta tagtttattt taaattgaat agtttattgt
    13801 tgatatattt tactattata tatttttatt gtgatataat agtaaaa
//
LOCUS       DAGQWR010000110        13843 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.110, whole genome shotgun sequence.
ACCESSION   DAGQWR010000110 DAGQWR010000000
VERSION     DAGQWR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13843
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.110"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            136..1641
                     /locus_tag="MIW95_002828"
     CDS             136..1641
                     /locus_tag="MIW95_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBY3152140.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            2031..3128
                     /gene="dinB"
                     /locus_tag="MIW95_002829"
     CDS             2031..3128
                     /gene="dinB"
                     /locus_tag="MIW95_002829"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBY3152141.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGKPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKSSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            3384..4520
                     /locus_tag="MIW95_002830"
     CDS             3384..4520
                     /locus_tag="MIW95_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152142.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            4513..6060
                     /locus_tag="MIW95_002831"
     CDS             4513..6060
                     /locus_tag="MIW95_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBY3152143.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            6394..7260
                     /locus_tag="MIW95_002832"
     CDS             6394..7260
                     /locus_tag="MIW95_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBY3152144.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            7401..8543
                     /locus_tag="MIW95_002833"
     CDS             7401..8543
                     /locus_tag="MIW95_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBY3152145.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            8927..10165
                     /locus_tag="MIW95_002834"
     CDS             8927..10165
                     /locus_tag="MIW95_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152146.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYNL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            10194..11081
                     /locus_tag="MIW95_002835"
     CDS             10194..11081
                     /locus_tag="MIW95_002835"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBY3152147.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYSLYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            11115..11744
                     /locus_tag="MIW95_002836"
     CDS             11115..11744
                     /locus_tag="MIW95_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBY3152148.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADKVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            11749..12213
                     /locus_tag="MIW95_002837"
     CDS             11749..12213
                     /locus_tag="MIW95_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBY3152149.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            12463..13830
                     /locus_tag="MIW95_002838"
     CDS             12463..13830
                     /locus_tag="MIW95_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY3152150.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIRYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
BASE COUNT         5237 a         1469 c         2381 g         4756 t
ORIGIN      
        1 aaaaaaatta atcttaattg tataatatta ctttgagtga aaaagtatta acattttttt
       61 ataagatgtt ttactaaaaa aatatatata aatatactgt tatcaacaag aaatttaatt
      121 tagtgaggtg ttaagatgaa agtaggattt gaccacaaaa aatatttaga agagcagtct
      181 aaatacatct tagaaagagt aaataatttt gacaaacttt atttagagtt tggaggaaaa
      241 ttgatgggag accttcatgc aaaaagagta cttccaggat ttgatgaaaa tgcaaagata
      301 aaagtattac aacatatgaa agaaaaagtt gaagttgtaa tttgtgtcta tgcaggagat
      361 atagaaagaa acaaaataag aggagacttt ggtatcactt atgatatgga agttttaaga
      421 ttaatagatg atttaagagg ttatgaacta gatgtaaata gtgtagtaat tactagattt
      481 gaaggacaac ctgctacaac tgtatttata aataaattag aacgtagagg aattaaagtt
      541 tataagcatt atccaacaaa gggatatccg tcagatgttg agactatagt tagtgatgag
      601 ggatatggag caaatgaata tatagaaact acaaagccaa tagtagtagt cacagcacca
      661 ggacctaata gtggaaaact tggtacttgt ctaagtcagt tgtatcatga aaataaaaga
      721 ggtaatgtag ttggatattc taagtttgag acattcccag tttggaatgt acctgtaaaa
      781 catcctttga atatagctta tgaagcagct acagttgatt taaaagatgt gaatatgata
      841 gattcattcc accttgaaac gtatggtgaa atgtcagtaa actacaatag agatttggaa
      901 ttatttccag tgcttaaaaa aataatagaa aagataactg gaaaagaatc aattttccaa
      961 tctccaactg atatgggtgt taatagagtt ggctttggaa taatagatga tgaagtaata
     1021 agaaaagctt ctatagaaga aataataaga agatacttta aaactgcctg tgagtataaa
     1081 aagggacaag ttgataaagg cgcttatgat agaatgaaaa tgattatgga agagcttaac
     1141 ttaaagccag aagatagaaa tgttgtatta cctgctagaa attacagtaa taaattaaaa
     1201 gaaagtgccg acaagaatga tacttgtcct gtagttgcat tagaattaga agatggtaca
     1261 atattaactg gtaagggttc tgatttaatg aatggaacag cagcagtagt attaaatgcc
     1321 attaaacatt tagccaatat atctgatgat atgcacttaa tatcacctgt aatattagaa
     1381 cctatcataa acttaaaaac aaagacttta gctagtagaa atgtttcact tagttgccaa
     1441 gaggtgttaa ctgcacttag tatttgtgct gtaactaatc caactgcaca gtttgctatg
     1501 gaaaagttag cattattaaa agggtgtcaa gctcattcta ctactatatt aaatagagat
     1561 gatgaacaaa catttagaaa gcttggtata gatgtaactt gtgacccaga atatccttca
     1621 caaaatcttt actatagtta aaattatata aaaaaatatg ggctgtttta aagtaatagt
     1681 ttgagcagtc cattacaatt ataataaaat cagtaggctg tcttgtataa gacagtcttt
     1741 tgtttttaaa acatcccata aattaaatat atatactgtt atccataagg aattttaaat
     1801 ttagttcctt ggaataccta ttgtaaagct tttatatagt ttttgcaagt gtttttaagg
     1861 attttttata aattatattg aagttaatgg attatagatg gttttattta tggaaaatat
     1921 gaatatataa agaaaataat gaaaaaatac caaaaagaaa cataagtttg gtataattaa
     1981 aaagtaatat aaataaaatt aatctattta tactttaaaa ggtgataata atgaaacatt
     2041 atagaaaaat aattcatata gatatggacg ctttttatgc atctatagaa caaagagata
     2101 ataaaaaatt aaaaggaaaa cctgttattg tcggtggaaa tccacaaagc cgaggtgtgg
     2161 ttgcaacgtg ttcttatgaa gctagaaaat ttggtatcca ttcagctatg ccatcagctg
     2221 tagcttacaa taggtgtcca tatgcagttt ttgtaagacc tagatttgat gtttataaga
     2281 gtgtgtcaga gcaaataaga gatatatttt atagatacac ggatttagtt gaaccattat
     2341 ctttagatga agcttactta gatgtaacaa aaaataaaaa aaatattgat tcatcaattg
     2401 agatagcaaa gcaaataaaa aaagatatat ttagagaagt tggtcttact tcttcagctg
     2461 gtgtgtcata caataagttt cttgctaaga ttgcatcaga cttaagaaag ccaaatgggt
     2521 taactgtaat aaccgaagaa aatgcccagg actttttaga taaattacct gtgaataaat
     2581 tttttggtgt tggaaaagtt acttcaaata ctcttaaaaa tttaggtata aaaactggat
     2641 atgatttaag gtgtttaaat ttatttgaac ttgaaaatat attcaaaaaa agaggttatg
     2701 aattatataa atttgctaga ggaatagacg atagaccagt ggaaccaaat agggttagaa
     2761 aatctgttgg ggctgagact acattaagtc ataatttgga tattgatgaa gaagaaacac
     2821 gcaacattct tgatgaactt tgtgaagaag tatgtcatag attaaaaagc tctgagaagt
     2881 ttggaaaaac attaacatta aaaattaaat atgaggactt tacaaagata actagaagcc
     2941 ttagtttaga acactatatt gacgaatata atgatataag aagtggtgta gataatttgt
     3001 tgagaaatgt tgaggttaat ggaaaacaaa ttaggctatt gggagttaca atatcaaact
     3061 taagtgataa aaaagaaact tataaagata ttacattatt tgaatacatg gatagtattc
     3121 aaatgtgagt tttgtttaaa tgaaataaca ctagctttaa tactatataa ttgaggctag
     3181 tgttatttta tgaatatttt tatacttttt gttttttgac attatatact atatataaat
     3241 actttatatt tgtagcaata ttcagacaaa tagtgaccta aaaatcgtac tttgtttaca
     3301 taaataaaat agtatgatat aattataaaa acaacttata atattggaga aggttgcttg
     3361 ttcgaaaaag gggatgataa attatgatag agataagaaa tgtaacaaaa aaaataggaa
     3421 ataatgttat attagatgat atttctctag ttgtagaaac aggtacattg gtagttttga
     3481 ttggttcaag tggttgtgga aagactacta ctttgaagct tataaacaaa ttaataaaac
     3541 ctacatctgg tgagatatat attaatggca aaccaatttc tcaggaaaat gaaatagagc
     3601 ttagaagaaa aataggatat gttatacaga acacaggcct attccctcat cttacaataa
     3661 aagaaaatat agagctaatt ccacgcctaa aaaaggaaaa atccgttgaa gaaattgaaa
     3721 aaagaacgtt gcaactactt gagatggtag gacttgatag tgatgaattt ttgaataagt
     3781 atccttcaga acttagtggt ggacagcaac aaagaattgg tgttgcaaga gccattgcta
     3841 ctgatgctga aataatattg atggatgagc cttttagtgc tttagaccca ataacgagaa
     3901 catctttaca agaacaatta ttttctcttc aagatgaatt gaaaaaaact ataatatttg
     3961 taacgcatga tatggatgag gctttaaaga tagctgataa aatttgtatt atgaaggatg
     4021 gaagaatagc acaatatgat actcccgaaa atatacttag aaagcctgca aatgactttg
     4081 taaaagattt tatcggagaa gatagagttt gggataatcc agaatatata aaagcaagag
     4141 atattatgat aaaaaatcct attgctgtta attccactag aactgtaact caaggaatag
     4201 aaattatgag aacgagtaag gttgatagct tactaattat agatagagca aaaacattaa
     4261 aaggtattgt tactgtaaaa gatatgaaag atattgatga taagtcaata ttacttgctg
     4321 atattatgtc gagtgagcct ttgcatgtaa atgaaggtga taatttagta gaaatactaa
     4381 atgtaatgaa tcgtaattcg gttggatata ttccggttat ttctgatgaa aataaactgg
     4441 tagggcttat tactagaagt agtttacttt ctgttttaag tgaacaattc ttagaaatgg
     4501 aggtgagtgt tcttggctaa tttttttaac tttatactat tacaaaaaga taagattata
     4561 gagcttttaa tacaacatat gagtctaact gttacatcta ttttaatagc tataatagtt
     4621 ggtgttcctc ttggaataat tatttcaaga atctcatctc ttagaaagtt tgtacttgga
     4681 tttgtaaact tagtccaagc agttccgtct atggcacttt tgggtcttct tgtacctata
     4741 cttggcatag gaagtaaacc agccatattt atggtagtgg tttattcatt actaccaatt
     4801 gtaaaaaata cgtatatagg tataacaagt attgacccag tagtattaga atcagctaaa
     4861 ggtattggtc ttacaagaaa tcagacatta tttaaaatac aattcccttt agcacttcct
     4921 ataataatgg gtggagttag aatatctgca gttacagctg ttggtttgat gactcttgca
     4981 gcatttatag gtgctggtgg tttaggatat ttagtatttt caggggttca aactgtaaat
     5041 aataatatga ttttagcagg ggcaatccca tcatgtataa ttgcactgtt agttgattat
     5101 ttatttagta aaattgaagt tgcagttact ccaaaaggat taaatccaaa agcacctaag
     5161 aaaaattata tagcacttaa agtaatatct gtagtggttg ttgtttcaat gttgttcgtt
     5221 gtttttagtt ctagtttttc aagtaaaaaa gatacaataa ctattggttc taaagattat
     5281 acagaacaac ttatacttgg taatgtttat gctgaattag tagaaaaaaa tacgaatctt
     5341 aaagttaaga agaatttaaa tctaggtggt tcatctgttg cgtttaatgc aattaaatct
     5401 ggtgagttag acatgtatgt tgactataca ggtacacttt tggtaaatgt aatgaaacat
     5461 gctccgatta aagatgctga tgaagcatat aatgttgtaa aagatactat ggaaaaagaa
     5521 aatcagttaa ctttacttga ccctcttgga tttaataata cttacacatt agctatgatg
     5581 cctgaaactg ctgaaaagta tggaataaat actatttctg atttgactaa atatggtaaa
     5641 gaatttacat tctcaccaac tttggagttt gaaaatagag aagatggtct tgtagggctt
     5701 tctagagatt atggtttaaa attcaaagat gtaaaagcaa tgaatggaag tttaagatat
     5761 acagctttag ataataatga atctcaggtt atagatgcat ttttaactga tggtcttatt
     5821 aaaaagttta atcttaagat attagaggac gataaaaatt tctttgtacc atattatgca
     5881 gcaccattag taagagaaga tactttgaag aaatatcctg agttagaaaa agttatgaat
     5941 atgctttcag gaaaaataaa tgaagaaact atgagagaac ttaactatca agtggatgaa
     6001 cttggtaaat caccagaaga agttgctcat tcattcttag ttaaagaagg cttagtataa
     6061 aaaattaata gtttatatca aaaatttaag ggagtgtctc aaaatgaact ttttagttca
     6121 tgaggcactc ttttttatat gaaatcaaat atattttatc atttcaacaa taaaaaagaa
     6181 gcttatcttt attttgagac agtctcttat ttttatgtat accaaaacca tacctacgat
     6241 ttttatttat aattaagttt caaatagaat gaacaagtac aaccaatttt gaatataaat
     6301 atagtaaatg tgcttatata cgattttatt atctgggtga ttgatattga tagatagctt
     6361 tctttattga atcgaaggga gggggaattt gttatggata actataagaa agtgaagcaa
     6421 gtattgtgga taattttatt tgctaatttt gcagtagcat tattaaaaat cattattggt
     6481 aatcaaataa aaagctatag tatgacagca gacggatttc attctttgtc agatggagca
     6541 tctaatatag tggggcttat aggtattttc tttgcatcta aaccaaaaga taaaaatcat
     6601 ccttatggac ataaaaagtt tgaaattata accagtttat ttataagtgg tatgttgttt
     6661 gttatagcaa ttaagataat tcttagtgca gtgctaagaa tagctaatcc agttgtacct
     6721 gctataacta tagaaagttt aattgcttta attattacac tttttataaa tatttttgta
     6781 tgcatgtatg aatatagaat tgggactaaa ctaaatagtt atgttttaat ttcggattca
     6841 ttgcatacaa gaagtgatat atttgtgtct ctaggtgtat tggttacttt agttggtgtt
     6901 aagttaggat ttccagtaat aatagaatct atagttccta ttattatttc tgcattcata
     6961 atttattcgg cttatggaat atttaggcca tctattggaa ttttggtcga tagagtggct
     7021 gtagatgagg attatataaa agaaattgtg tttgaattta atgaggtaag ggatgttcac
     7081 aatatcagaa gtagaggtag caaaagttct atttatattg acatgcatgt aatggtagac
     7141 ccatttataa gcgtagagca atctcatgat ttaacacata aaattgagaa acaaatacaa
     7201 gaagaaatta atgaaaatgc tcaagtaata gtccatattg aaccttttta tagtttttaa
     7261 cttagctata aaatacaagt tataaaaaaa tataatttat aaaaaaaata ttataccata
     7321 aataatttat aaaaattatt tatggtataa tattttctat aaagaaatta agtcttaata
     7381 tatctaagga ggaatttaca atgagatacg ttgaatatgg aaagacaggg aaaatggttt
     7441 cagtagttgg ctatggagga ttaaggtttg atttagaaaa aagtaatcaa gaaaatgctg
     7501 atttagtcaa atatgcgtat gaaaaaggta ttaattactt tgatacagcg ccagggtatt
     7561 gtgatgacag aagtgaagat atatttggac tagcctttag acaaatgata aaggaaggta
     7621 agaccgattt ttatgtatca actaaagaaa agccaaaagc atgtgataca tcagaaaaag
     7681 ctatagaggc tgtaaaaaaa tcaatagaac gattaggcgt atctaaaata aatttttatc
     7741 atgtttggaa tattagaaag atggaacatt acgaactagc aatgagacct ggaggtcaat
     7801 atgaaggatt attgaaatgt aaagaagaag gattgataga ccatatagta ttttcatctc
     7861 atcaaccagg agatgaggtt atagatattc taagtgaaaa taagtttgaa ggtgtaacta
     7921 tgggaataaa tatattaaac tttccatata ggctaaaagg tgccaaatat gctgttgata
     7981 atggatatgg tgttgtggca atgaatccat taagtggtgg aactattcca aagtataata
     8041 aagaactttc ttttctatca aaagatggcg aaagtactat agaatcagct ttaagattca
     8101 acataggaat ttcacaaata agtatatctt tgatagggtt taataaaaaa caagatatag
     8161 atgatgcatg taaaattgca gacgaaaata gaatttattc agatgaggat atacatgata
     8221 ttgagactag attaaataag aatatgaacg aaatatgtac aggttgtggt tattgtaaag
     8281 tatgtccaaa aggtattaat accccagcct atatgctttt ttataatgaa aagcagatgt
     8341 ttaaaaagag tgatgaagaa atgacaaagt tagtttatgg tttaggacat tggaattaca
     8401 caatgaatag taaggcaaaa gctaaagaat gtatatcttg tggaaaatgt gaagttgaat
     8461 gtactcagca tttacctata atagatagat taaaagaaat taaaaaatgg gaagaagatg
     8521 gagctaatag tgttaaagtc taattaattt tatactatat ttgaaatttg tatagtattt
     8581 agtaatgttt taaaatatag ttttaatatt taaggagaca gattataatt ttgtctcctt
     8641 aaattatgct ttaatttgtt atagtttaat tccataagca acattgtata tttttatata
     8701 aagtttttca aatattttac attaatattg cttacaaatt ctcattttat acaacactaa
     8761 tctatgttat ataaacaata cttgactgat tataacatca ataaagtaaa tattgtggtt
     8821 gtaaataaag agttattcaa ttacaataaa acaagtaaga acattatgaa gatggctatt
     8881 aaatataagg aataaattgt aggtgtcagc aaatcctata taatgaatgg atggtgatat
     8941 attgatgagt aaaatattaa aatataaaaa cgagatgttt ttgtttatat tagttgtaat
     9001 aacttactta ataataacta agatattttt ttctaagact actatatttt ataatttaaa
     9061 caatacttat gatgtattgt tagatacaga tacaggggtt ttattcaatt taaatgtatt
     9121 tgctataagt caagacaata gtaagcacat attgttttca gcaattatat caatttttgc
     9181 atatccaata tacctatttt gtacttcaat agcaaatcct gggacaacag attttaatag
     9241 tgcctatggg tttggactaa tttgtctaca aataataact agtgctatgt caataacttt
     9301 agtctttaat catattaaaa agataaaaat gcagagatta actttaattt tacttactat
     9361 gataatgata ttttcttttc cacagttatt tatgacttta aatgtagaga gatttatata
     9421 tagccaattt tcattaatat ttttcatagt tattgcaaat aagatgaaag gaaaaaattc
     9481 ttatttagta gaattagcag ctattcctct atttggaata actattagta atatatattt
     9541 gtattttttt aatatgatat ttgagtttaa attaaaaata ggaaaaatgt taaaacattt
     9601 aattacattt atcctaatgg catatatttg tgtagtttca acgaagtctt atgagagttt
     9661 tatgaacctt ggaaatgtaa tacagtatga tacgaaattt ataagtggag aaccaatttt
     9721 ggaaaagatt gcaatgatta tagagagatt attgtattcg gttttttatt ttcctggagc
     9781 aagtattaaa aaagggttat ttttgcaaaa tggagaagtt gctacaatac cagtaattct
     9841 tacattatta gctttatgtt tttgtgtttt atcagttatt gaaaattcag aaaaaagagt
     9901 tcctaagcta tgtatgggaa taattatatt caatctaact ttacatggta tagtaggata
     9961 taatctagtc aattcaagta ttatggctat aaattttagt tttgcagtta taattttatt
    10021 ggcttacttt acgaaagcgc ttagaaaaaa tgaaaagaat atgtataata tatttctatc
    10081 gctgttattg gttactatag taatcagtaa tgtcaatgga tttatagaaa ttttaaacat
    10141 agggataaaa tcatatcctg tttaaaaaat aatattgaaa gggtagtaag tttatgaaac
    10201 agaccaatac aagtataagg cttcttacta aaatttcaga ttatataaag ttaatgagaa
    10261 taaagcaata tataaagaat ttctttgtat tttcagctat aattttttca aacaacatac
    10321 taaatataaa tttattttta aatacgttta ttgcgtttgt gtgtttttgc ctaatgtcat
    10381 catcagtata tgcactgaat gatgctatag acatggataa agataaaaaa catcctaaaa
    10441 aatgtaatag accagtggca agtggaagaa taagcaaaaa aagtgctaat atactatttg
    10501 tagtattagc tttaatttca gtctgtttat caacagttgt tagttttaat ctttctataa
    10561 ttcttagtct ctatttgata aacaatatat tatattctct taaactaaag aatataatac
    10621 tattagatgt gttttcaata tctcttggtt ttatacttag ggtttatgca ggatgtgtag
    10681 ccataagtgt tagtttatca aattggataa ttttatgtac atttttctta tctctgtact
    10741 tagctcttgg aaaacgtaaa aaagaaatag aaactctaag agatgatgca gttgaacata
    10801 gaaaaatatt ggaagattat gacatagaaa atctaaatca aatgatgata gttatattaa
    10861 gttctacaat tgtatgttat tcattgtatt ctacaagtaa tccagaaaag ccacatatga
    10921 tatttacaac aatatttgta gtttatggtg tacttagata taattatata attaacacta
    10981 caaatgaaaa taatcctact gatattgtcc taaaagataa tgctttaaaa ataaacgtaa
    11041 tcttatggat aattacatgt ttaataatat tgatttttta gtgatatttt tagtgatagg
    11101 atggggaaat acttatgaaa aaaagtgtat tattacttga tgtagattat actgttataa
    11161 atactgatag catgatagat ttttttattt actctttaaa aaataaaacc tttaaaacta
    11221 ttataaagtt gccatacatt atttttattc tatttatgta tttaattaga atgataccct
    11281 tgaagaaggc aaaagaggca atcttttatc caatagtaga ttttagtgaa gaagatttaa
    11341 aaaagttttt tgatgattgc ataatgaaaa aaattaacga atctatgaaa aaggtcattt
    11401 acaaaaataa agaagaagat aatgttataa ttatgataac tgcatctcca tatgcatata
    11461 tgaaatattt taagtattat ggtcttgcag acaaggttat aggcacggag tttttttatg
    11521 aaaattcaag atataaaaat caattcattg gagaaaactg taagggaatt gaaaaagtaa
    11581 aaagaataaa ggctgtatta ggtaaacttg ggatagagat tgattatgaa aattcatatg
    11641 catattcaga ctctaagagt gatttaccaa tgctttcatt gactaaaaat gcatttttag
    11701 ttagtaaaaa ggatggtgag gttatagagc aaataaattc ataacttaat gtcacttaga
    11761 ataggaggga atagtttggg atatttacac atattaattt tatctatggt tcctgtaaca
    11821 gaattgaggg gagccattcc tataggtatt gcaatgggct taaatcctat atgggtttat
    11881 gtatttagtg taataggttc tacattggta tcgattccct tgatattgac attcagacat
    11941 gtattacaat ttttaagagg gaagaaacta tttaaaggga ttgctgatgt aattgataga
    12001 aaaataaact caagaatgaa gaaattaaaa agtgtttcga taattggtat aatactattt
    12061 gtaggtatac cactgccaac tacaggaaca tggactgcat ctgctatagc ttccatatta
    12121 aaaatgagaa taaaagatgc ttttatggga gtatttcttg gcaatttgtt atcaggggtt
    12181 atagtttcag ctctttcttt acatataata taaaactatc taagtaatat acttggatag
    12241 tttttttatg agtacatgtg aaaaattttt aataataaat aaaaaatgag ataaagtatt
    12301 taaattcagt tagttctaaa atgtttacta tggcaaaaga aatatagtat agtattactt
    12361 gtagggaaag tactctaaat ttgtctgctg ataaaatgaa atttttatat agaataaaat
    12421 tttagtgata cattttaatt attggattga ggaggtttta aaatggaaaa ttcaaacact
    12481 aacttaggca gtgaaagtgt gggtaaatta ttatttaaac ttgctacacc tgctattatt
    12541 gcacaaattg taaatgtttt atacaacata gtcgatagga tttttattgg tagaatggaa
    12601 aatggagaag ttgctatggc tggtgttgga gtggcctttc caataataat tataataact
    12661 gcatgtagtt atttaatcgg tatgggagga ggtccattag ctgctataaa aatgggtgag
    12721 caaaacaatg atgaggcaga gaaaataatg agtaacagtt tttctgtact ggtgatttta
    12781 gcaatacttt tgactatagg ttttaagatt ggaaaagagc ctttactttg gatgtttgga
    12841 gcaagtgaat ctacaattag atattcaatg gactatttaa atatttattt aataggtaca
    12901 gtgtttgttc agatttcaat gggtatgaat acttttataa atacccaagg gtttgctaca
    12961 actggaatga tgacagttgc tataggtgct ttaataaaca taatacttga cccgattttt
    13021 atatttggct ttaatatggg tgtaaaagga gctgcattag ctactataat tgcacaaggc
    13081 gtttctgcta tttgggtact tatgtttctc tttggaaaga aaagtatact taaaataaaa
    13141 aagaagtata tgataccaaa agcaagtatc atcttaccag tgcttggctt gggaatctct
    13201 ccatttataa tgcaatctac agaaagttta gtacttatag ccttaaatag taaattacaa
    13261 atgtatggag gagatttagc agtaggttca atggccataa tgagcagtat aatgcaaatt
    13321 cttatgcttc ctaatatggg ggttacacaa ggagcacaac caataataag ttataattat
    13381 ggttcaggtc aacttgatag agtaaaaaag acatttaagt tgtgtttatt gtcatgtttt
    13441 acatattcta ctattttatg gcttttatta atgatatttc ctgcattttt tgttagtata
    13501 tttaataaga atccgcagtt acttagtatg actagttgga gtataaaaat atattttgct
    13561 ggtgctttta tgtttgggat tcaaatagct tgtcaacaga catttttagc acttggaaaa
    13621 gcaacaatat ctttggtttt agctctactt agaaagattg tacttttaat acctttaata
    13681 tttatactcc caacattctt taatgaaaaa ctttttgcag taattttagc agagccagtt
    13741 gcagatataa cagctgctac tataacagca atttctttct ttatatttta taaatgtttt
    13801 ctatcaaaac ctaaagcaat aaaagaataa gatatacaat ata
//
LOCUS       DAGQWR010000111        13472 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.111, whole genome shotgun sequence.
ACCESSION   DAGQWR010000111 DAGQWR010000000
VERSION     DAGQWR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13472
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.111"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            229..642
                     /locus_tag="MIW95_002839"
     CDS             229..642
                     /locus_tag="MIW95_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3152151.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKTEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
     gene            833..2050
                     /locus_tag="MIW95_002840"
     CDS             833..2050
                     /locus_tag="MIW95_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBY3152152.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            2212..2910
                     /locus_tag="MIW95_002841"
     CDS             2212..2910
                     /locus_tag="MIW95_002841"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBY3152153.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(3130..3747)
                     /locus_tag="MIW95_002842"
     CDS             complement(3130..3747)
                     /locus_tag="MIW95_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBY3152154.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     TVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(3874..4896)
                     /gene="argF"
                     /locus_tag="MIW95_002843"
     CDS             complement(3874..4896)
                     /gene="argF"
                     /locus_tag="MIW95_002843"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBY3152155.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAYVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQKIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            complement(5005..6192)
                     /locus_tag="MIW95_002844"
     CDS             complement(5005..6192)
                     /locus_tag="MIW95_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBY3152156.1"
                     /translation="MNNNNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            complement(6222..7082)
                     /gene="argB"
                     /locus_tag="MIW95_002845"
     CDS             complement(6222..7082)
                     /gene="argB"
                     /locus_tag="MIW95_002845"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBY3152157.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGMDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            complement(7097..8314)
                     /gene="argJ"
                     /locus_tag="MIW95_002846"
     CDS             complement(7097..8314)
                     /gene="argJ"
                     /locus_tag="MIW95_002846"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBY3152158.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKENDVLVASTGVIGKPLNINLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            complement(8356..9390)
                     /locus_tag="MIW95_002847"
     CDS             complement(8356..9390)
                     /locus_tag="MIW95_002847"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBY3152159.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKNI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEVL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     misc_binding    complement(9644..9857)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10080..10844)
                     /locus_tag="MIW95_002848"
     CDS             complement(10080..10844)
                     /locus_tag="MIW95_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HBY3152160.1"
                     /translation="MQIIDININNIDSRHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     AFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKGFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKI"
     gene            11147..11620
                     /locus_tag="MIW95_002849"
     CDS             11147..11620
                     /locus_tag="MIW95_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3152161.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFEDIAVEQIVDGIRFTPMKLLI"
     gene            complement(11989..12546)
                     /locus_tag="MIW95_002850"
     CDS             complement(11989..12546)
                     /locus_tag="MIW95_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY3152162.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
     gene            12804..>13472
                     /locus_tag="MIW95_002851"
     CDS             12804..>13472
                     /locus_tag="MIW95_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY3152163.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHEYQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTILENGKHWFNTPQ
                     ELNSL"
BASE COUNT         4575 a         2146 c         1502 g         5249 t
ORIGIN      
        1 attcaacaat ttaggtttac taaatttatt gatgatttct ttttatataa cttaaactac
       61 atttttattt taaaaaattt aatatcaaca atataaaatt aagaataagt attattagtt
      121 tgacattagc ataaatgcct attagaataa tgttaaacta tattctttct actgatttta
      181 ttaaaaaata ccaatataca aaagattata caaggagaaa gttcaaatat ggaattagaa
      241 attacagaaa aaatagaaaa acaagatgaa gatactattt ttcaaggatt attaaaatat
      301 aatcttgcta gacttgaaga taaaaaccca gtggatttag gaatatatat tcgagataaa
      361 gcaggtaaaa ttgttgcagg ttttataggt gttactcatg gtaattggct ttctataaaa
      421 tacttatggg ttagtgagaa gcttagatat aaaggtactg gaagtcaact tttatataag
      481 actgaaaaaa ttgctaaaga acgaggttgt aaatatgttt ttttagatac attcagtttt
      541 caagcaccta aattttatga gaaatttgga taccataaag tattttcact tgaaaattat
      601 cctctaactg gaaagagata ttattataga aaaatactat aataataaaa atcatatttt
      661 atatttttaa cttaaaactt ctgctcttcg tctacagact ttatgttttt atatcattat
      721 taatactaaa atagtatttt actatacata caaaattatt tgaaagttca aaaattataa
      781 gtataaaagc taatcttagc ttaaataaaa attatatttt taggaggtaa atatgttagg
      841 aaaaaaatgt atggattatc tacaaacatt aggaaaaata agttcaacaa caaatggtct
      901 tacaagactt atcttgactc aagaacataa aaaatctatt gatttaatta gttcatggat
      961 ggaaggttta aatcttgata ttgaaattga tgacattgga aacgttatag gtacatataa
     1021 atcaagcttt ccaaatgctc ccacattagt agttgcatct catcaagata gtgtaaagtg
     1081 tggaggtata tttgatggta tgttaggtat aatagtgcct ctagttggtc ttgaagaagc
     1141 aaaacacaat aataggtctt atcctttcaa tattaagcta attgctttcg ctgaagagga
     1201 aggaactaga tttgaaactt ctttaatggg aagtaaagtt tttgctggta cttttaaaga
     1261 ggaacttctt aaatctgttg atgaaaatgg aattacactt gaagaagctg taacaaagtt
     1321 tggatttaat acaaaaaact taacaaattt gcatcctaga aaagatgtag atgcatattt
     1381 agaatttcat atcgagcagg gtcctgtttt agaaaatgaa tctcttcctg ctggaattgt
     1441 atcaagtatt acaggattta aaagttttaa gatatcagta aatggtaagt ctggacatgc
     1501 tggtacactt ccaatgaata tgagacttga tgctggatgt tgtgcctgtg aatgtgtatt
     1561 agctatcgaa aaagtagcta aaacgactgc tgatttggtt gctactgtag gaaagatgaa
     1621 tttctatcca tcttcatcaa atgtggtacc tgaaagagct gaatttacac tcgatgtacg
     1681 ctcttgttca caagaaattc ttgataattc tgtagaaaag atatttaatg aaatatcaca
     1741 tatatgtgaa aatagaaaac ttaattatac aagtgaactt gcttttgaaa atgttcctgt
     1801 accatgttct aataaaataa caaaaatcat agaaaaaagt tttatagatt taaatcttaa
     1861 tcctttttat atttatagtg gtgctggcca tgatgctcag gaaatggata atataactga
     1921 tattggtatg gtatttataa gatgtgctgg gggcgtaagt cataatccta atgaaagtgt
     1981 ttctgtagat gacttggata ctgctgtaaa aatattttta aaaatactag ataatcttga
     2041 tttaaaataa tttttatata agaaagtata ttaaaatata taatctaaaa aatcacttta
     2101 tatttatatt atacaagtgt acaatataat ctattataaa atataatttt atgcttttat
     2161 agatttttat tttatacata aatatattta tatataaagg agataatttt tatgaatgaa
     2221 aaattaattg gtattttggc tggaatgggt ccaaggtcaa cagccccttt tgttgattta
     2281 attattgatg agtgtcaatc tcaatatgga gcaaaatatg atgatgaatt tccaaagatg
     2341 atgatatatt cattacctac cccattttat attgaccgtc caataaatca tgaacttatg
     2401 aaagaaacta taattgatgg attacaaaag cttgaatcaa ctggtgtaaa ttttattgca
     2461 atgccatgca attcagcaca tatatacttt aaagaattaa aagagtctat taatatacca
     2521 atattaaata ttgtagaaga aacagttaaa aaattaccta ctatctcaca aaaagtaact
     2581 cttttttcga ctagttcaac ttttgagtct acaatttacc agaatggtat aatacataat
     2641 ggacatgaat ttatatttaa agatgaatgg caaataaaat taaacaatct tattcaaaat
     2701 ataaaaatgg ataaagaaaa tccacacaat attgatattt ggaataaact tatcgaagat
     2761 gttaaaaatg aatctataga aaatatagta attgcttgta ctgatttaaa tgtagtttta
     2821 gaaaaatcat atccttcaat aaatattgtt gattcatcaa aatgcttggc tgaatcagtt
     2881 gtcaataaat atctaaaatt agttaaataa atttttcatg cactatatta taaaaaatat
     2941 atgttataag tttattttaa atagtataga cttataacat atatcatagt ttcttgacaa
     3001 tatagaaatt aaccatgtat ttcattatat taataaaaag gataccaaaa atggtatcct
     3061 tcataaatat ttatatgttt atttataaaa ataatcatat atttattact ttactacacc
     3121 taaacaatat tattcaagtg tattcataga gttagaaaat tcttgtcctg ctgtaacaag
     3181 attagataac aatacatcag ctactgcatc atttactgaa cttattatcc atttattatc
     3241 ctttttagaa cattcaaact ctacagtttt tgtaacataa gtctctttaa cgcttttttg
     3301 tttttctttc ataattgata ctaacatttc agttgttttc tcatcagtta agtcttgtcc
     3361 tgaaaaactc ataccaatca tctttgtaaa tgcctcagct actatttctt gaagtaatgg
     3421 tgttgaatct ataaatttac attctacttt gattgtagcc ttatcacctt taacctcgct
     3481 gtcttttatt gtatacgtga ttttagatgc attctgtttt agataatcta aaacatattg
     3541 ggcatttggg tcttttgtat ctaattcctt taatatatcc ttatattttt cgtcattatt
     3601 ttccattacc ttattcatgc cttcaaagtc aaactttttc gcactgttaa aaaaactatc
     3661 aattgtatct tctggttttg cacttgaaca tgctgttaaa gatattgcta ataaaaaagt
     3721 tgttaaagct aatatgattt ttttcatatc tcaaatcctc cctcttaatt tgttgataca
     3781 taagttattt taacatataa tgagagtttt tgaataatat atttaaaata ttttataaat
     3841 tttcattaaa gtaaagtcta tattaaaaca tatctatatt aaattattgt tcatatttac
     3901 atcagtcata gtagaaacca taacagcttt tattgtatgc attctatttt cagcttcttc
     3961 aaataccttt gaatattttg aattaaatac ctcgtttgta acctctaatt ctgataatcc
     4021 gaacttttca taaatcttct gagcaacctt agtttctaaa tcatgaaatg ctggaagaca
     4081 gtgtaaaaat ataacatttt cattttcagt tttctttatc atatccatat ttacttgata
     4141 atctttaagt tgatttattc ttgcttcata ttgctcctct tctcccatag aaacccatac
     4201 atctgtatat ataacatctg cacctttaac atcatcaata ttttcagtca acattatatc
     4261 tcctttggat ttcttggcta tttccttcat ttccttaacc aattcttcat ttggccaaag
     4321 ttccttaggt gctagtgcta cgaaatgtat tcccatttta gcacatccta tcatcagtga
     4381 gtttcccata ttatttctag catcaccaac atatacaaat ttaactttat tcaaatcttt
     4441 atctacattt tcaattactg ttaaaaaatc tgccagtatt tgtgttggat gatattcatc
     4501 tgtaagtcca ttccaaacag gtacaccaga atattttgct agactttcaa ctgtttcttg
     4561 tttaaagcct ctaaattcta ttccatcata gaatcttcct agaacttttg ctgtatctga
     4621 tacagactct tttttaccca tatgacaatc atttggacct agataagtta catatgctcc
     4681 ttcatcatga gctgctactt caaatgaaca tctagttctt gttgatgttt tctcaaataa
     4741 taaagctata ttttttcctt gtagcaaatc gccttttatg ccaacttttt tcttagtttt
     4801 taaataactt gataaagtta ataaatatct aatctcttct tttgtaaaat cctttaatgt
     4861 caaaaaactc ttacctttta aacttatatt attcatatta attcgcccct tcttataaac
     4921 ttcttaaatt caattaaact tgttagcact ttactttttt atttacttct aatttttatc
     4981 tttaattaac tattttttga atatttagtt aatctctagt aatatatctt ttaggatttc
     5041 aagagcctca tctatttcct tataactgat tgttaatggt ggtaaaaatc tcaatacatt
     5101 ttttccagca gtcaaaataa gcaatccttt ttttctagct ttctcttcta taatactaga
     5161 ttcaatatta gtttcaattc ctatcattag accaatacct tttgtcttta atacaacttt
     5221 attattaaaa ctttcaatag attttcttat ataatttcct ttttctaata cttcattata
     5281 aaaaccatct ttagatatct catctaatac aactgaagct gccaccatag ctaataaatt
     5341 acctccaaaa gttgtacctt ggtctccttt tcctagtact tttgaaagtt ttttatttac
     5401 caatactcct cctactggta ttcctgctcc caaccctttt gctacggtaa ctatatctgg
     5461 ttttacatca aaatattcat agccaaaaag tttaccagtt cttcctatac cgcattggac
     5521 ttcatcaaaa atcactacaa tatccttttc ttgacatatt tttactattt ccaaaacata
     5581 atctttttca agaacattaa ctccaccttc gccttgaatc gcttcaagaa ttattgcaca
     5641 aactgtggaa tcaagattat tttttaaatc ttcaatatta tttctttcta tatatttaaa
     5701 accttcggga agaggataaa aatacttatg gtatttgtcc ataccagttg ccattaaaga
     5761 catcattgtt ctaccatgaa aagagtcttt taaacttatg atagttcctc taccattacc
     5821 atatttgtca ctactatact ttcttgctat tttgaatgct gtttcatttg cttctgttcc
     5881 agaattacaa aaatatactt tagccattcc acttgaatta attattttct ctcctgaata
     5941 caacactggc tcattaaaat ataaatttga agtatgtaat aatttatcta cttgctcttt
     6001 tagagcattc tttaaattac tattactata cccaagagaa gatactccat atccacttgt
     6061 aaaatctatg tatttattgc cattagtatc aaaaaaacaa cttccttcac cataatctat
     6121 gacaaaattg ggttggttat atgtatctat aaaatattca ttccattttg aaattgtatt
     6181 gttgttattc attcttattc tcccctctta aacttataat tttattcatt ttcttttctt
     6241 attaatgtac ctattccact atcagtaaat agctctgtta ttatagaatg tggaactctt
     6301 ccatccaaaa tatgtaccct atttactccg ttatttaagg catctacgca tccctctatt
     6361 ttaggtatca tacctccagt aattatacct tcttcaaata atttatcaac atcctctaat
     6421 ataacttctg ttattaatga tttttcttca tctggctctc ttaacaaacc agggacatct
     6481 gtaagaagta tcaatttatc tgcatttaac tctttagcta tagcagaagc tgctgtgtca
     6541 ccatttatat tataagtctc tccatcatct ccaacaccta tagttgcaat tactgaaata
     6601 taacctgact ttagacaaga ttctataagc tcaacattta cttttttgat ttctcctaca
     6661 aatccaagtt cataattttt atatgggtca cataaaatca tattattatc cattccacaa
     6721 agccctactg cttttccacc ttttgtatgt attttattta ctaggtcttt attaaccttt
     6781 ccagcaagta ccatttttac aatttccatg gtctcttcat ctgtatatct aagaccattt
     6841 acaaatttac tttctatatc cacttttgct agcatcttat tgatttctgc ccctccacca
     6901 tgtactaaaa ctatattgat tccaacataa ctcattaata caaggtcttc cataacagat
     6961 tcttttagac catctttttt cattgcactt ccaccatact taactacaat agtctttccc
     7021 tgatgttttt ctatatatgg taaagcttct ataagtgtat ttgctttttc tatattaatc
     7081 attaaagtat atcctcctaa tttctataac ttccatttat tcttacataa tcatagctta
     7141 aatcacatcc ccatgcattt cctctggcat tgcccatatt taaatctact aatatactta
     7201 tttcgtcatc ttcaaggatt ttttttgctt tttcctcatt aaaatctaat ccatttccat
     7261 ttttacatac ttcaatatat cctttcatac tctcaaaaat aatatcaact ttttccatat
     7321 ctatttcttc acctgcataa cctaaagcac acagtattct accccaattt gcgtcagctc
     7381 caaatacagc tgtctttact aatgaagaag atataactgt ttttgctaat tttactgcat
     7441 gttcttcact taatgctcca tttatataac attcaattag ttttgtacat ccttctccat
     7501 cttttgcaac aagtttagca agttctatac agacatatgt aagtgcactc aaaaatactt
     7561 gataatgttc atcttttttg tttattctat cattttttgc ttttccattt gctagaatca
     7621 tcaccatatc attagtactt gtatctccat ctacactaac tctattaaaa cttttatcaa
     7681 cagcaatttt caaagcttct tttagcaaaa ctccatcaat atttgcatct gtagttataa
     7741 atcctaacgt tgttgccata ttaggatgta tcatgccaga accttttgcc atacctccaa
     7801 tagttatttt tttatctcca tacattattg ctactgctat ttcttttttt ataatatcag
     7861 ttgtcataat agcttctctt gctccaatat gaccttgttt acttagatta ttcactaatt
     7921 catccatatt ttcttctatt aaatttatat ttaatggctt tcctatgaca cctgtagatg
     7981 caaccaaaac atcattttct ttaatattta atttatcact agtgaattta gccattttat
     8041 aagcatttat aagtccttct tttccattaa aggtattagc attagcacta tttactatga
     8101 tagcttgtgc ttttccattt ttaatgtgtt cctgtgtaac ataaacagga ttgcccttta
     8161 ctttattttg agttactact gctgctgcat cacataaaac atctgaataa accaaagcta
     8221 aatctctttt ttctttattt tttctcaagc cacagtgaat ccctgaagca aaaaatcctt
     8281 ctgaaactgt aactccaccc tttataatct ccatatttat cctcctaatt atatgagttt
     8341 agtatttttt cattactaaa atgatggtgc aatatttttt aaacctgtat tttcttcaat
     8401 accaaatatt atattcatgt tttgtatagc ttgacctgct gctcccttaa tcatattatc
     8461 tatagctgaa cagattatca atgtatctcc attttcatgt aatgatatga cacatttatt
     8521 tgagaaccta acattcttta aagaagctac cttatctaat gggagaactt ctataaattc
     8581 tttaaattca taataatcag ttaattttct atgtatatca tttatagaaa tattatcttc
     8641 ttttgtacaa tatatagttg ataagattcc cctatttact ggtattaagt ttggtgtaaa
     8701 aactacactt actttttctt tgcaggattc acttaaattt tgttctattt ctggagtatg
     8761 tctgtgtttc cctattttat atgcagtgat attttcatta acctccgtaa agtgactact
     8821 aatcgataat tctctaccag cacctgtaag acctgattta gaatcaatta ttatattatt
     8881 attttttatc aatttagaac ttagtaatgg catcaatggt aaagaaatac tagttggata
     8941 acatccagga tttgcaatta tatctgcatc ttttatatct tctttgtata tttcacttaa
     9001 tccatataca gcttttttat gtaagatttt atctataaaa gatactccat accatttact
     9061 atatacttct tcatctttta ttctaaagtc agcacctaaa tctataactt tctttttcga
     9121 tttaattgct tttattacaa acttttcact aactccatgt ggaagggctg taaacacaac
     9181 atcacacata tctataactt tttcattttc tatacatatc atgttatttt tatatcctat
     9241 acttggatat atatcaacta tattttttcc aacatacgaa tttgaaccta ttgctgtcac
     9301 ttcgacttta tcatgattca ttaataatct aatcaactct gccccgacat atccagttgc
     9361 gccaataata ccaactttta ttttattcat tttgttccct ccatttttat attcaaatag
     9421 tattaaaatt ctatctttcc actttactat atgataatca tttgataata tgtagattaa
     9481 aatattctaa ttaaaacaaa aaacccctta gcattataaa tactaagagg cgattttccg
     9541 cggtaccact ctcattggta aaattacaat aatcattaaa actcaaatta aaaagtatct
     9601 acaaaatgta catttaaaat taaaaaagcc tcttggtcaa agaccaagag acgaatatac
     9661 acccgcgtta ccactctaat tgataagata atcttaccca ctctaatctc ttaaggcgag
     9721 attcaccaat taccatacta ttacttctgg taatctcctc caaagctcta ttcacaataa
     9781 aattcgttac taggcttcca ccattcccag ctctctatca actagcttta tgctactctc
     9841 tttttcacag gatttcaatt tattttctta ttgttttata tgatagttta tttttttaac
     9901 tttgtcaata taatttttaa atttttggaa atattttcta tatggttaat ttccagcatt
     9961 ttgtatctaa attgagttat aatctttata ttatcattca tcaacttaag aaatttccaa
    10021 aatatattgg attataaaat aatgtaatct caaaatacaa tatttacatc tatttttagt
    10081 taaatttttt taatgttggt atctaaaaat ttctcaatct ttttctcaga aagtatctcg
    10141 ttggttacaa attcaccatt aaaaaaaaga ctgtatgtag taaatggtgc aggagcattt
    10201 tgtgcttctt ttgctgtttc gaattttata agatttacat caatacctct agaatttgca
    10261 atttttttca aaataggagc atatttttct gtatgtggac actgattact gtaatataaa
    10321 actataccgt tctcatttaa ttttcttttt tttacacaat tcttaaaaca tggaatagga
    10381 gcattatcag taaagggaaa atataatagc tcataatttg gttctgatgt atctgctaaa
    10441 caaaagccct tatattttag atatttagca tcagataaaa aaggcatttt tttactagac
    10501 gaaagcacaa ctaatccata tttacctttt gattttgcat catcaacaca ctcttgtagc
    10561 aatagattag aatatccttg acccttaaat tttccagata tccaaaaaca gttaataaat
    10621 atataattgt ccgcagtaat aggacaccat gcattttctg ctggaatata ttctataaaa
    10681 gctttacctc ttacatcaaa tcgtttaaag gctagacctt tatcaaaatt atttttcatc
    10741 caagcttttt tagaaaaaac acaattttct cctttttttt cagaaattga acagcatata
    10801 tgtctactgt caatattatt tatatttata tctataatct gcatacttat ctccttttct
    10861 aattacataa taatttttaa atatattcta tatattttta tcttaatatt ttgaatacaa
    10921 caacatcatg tcatatatat ttttatttta cataatatta tttttagatt atttgaatta
    10981 tcaaattgca tatataatta aatacattct aggtctaacc acactatata taaactatat
    11041 attatcataa tattctaaat ttataaaaca aattgtattg aattaaattt atttaagata
    11101 tataattata attataatta aaataataat aaaggtgttg tttataatgt tcaaagaata
    11161 cttaataaga gaattaaaag aagatgatat taaaaatgca ttagacttaa tctggaatgt
    11221 atttgaagaa tttgaatctc cagattattg tgatgaagga attacgacat ttcatcaatt
    11281 tatagaatat aattctatta ttgaaaaaat caaagaaaaa acactcttct tttggggttg
    11341 ttttgactct aaaaaactta ttggagtctt agctattaga aatttaaatc atatatgtat
    11401 gttatttgta gataaaaact atcataaaca tggtgttgca aaaaaattaa tgactacagg
    11461 catacaatac tgtatttgta ataaatttaa tttaactgaa ataactgtaa attcatcacc
    11521 ttatgctgtt gaagtttatc ataaattagg atttgaagat attgctgttg agcaaattgt
    11581 tgacggtata cgttttacac ctatgaaatt gttaatataa taaattaaat aaaaagcaaa
    11641 gtctcttaaa tattttatag ttactttgct tttaaaccaa gatttaaaat aatactatct
    11701 tttttgtttt ttaataatat actttaaaag ccttactaat atgaactata atttattttt
    11761 tgatatcctt tttgattaac atataaatta attcaattat taaacataaa ctatgttgtt
    11821 tctctttata tctcttcttt gtaaacaata ttgggcattt gaaaataaat attcttaaaa
    11881 tatactgttc tcctgttgat tttcctttgg tacaaatgat aaaattttaa tttacatgct
    11941 ccgtaatgaa gtgtttaata aataagttaa aataatttat ttacctcctt aatcatattt
    12001 tttatgaata cctaataaca cagtatcaat agaatcggct aaacctttta tagcttcttc
    12061 tttactcaaa agtgatttcc caagttgcaa agtcgaataa ccatgaagta cactcattat
    12121 taagtttaat acttcacctg tattctttgt atttagatta caagaaagaa taagtttttc
    12181 taataaggat ttatattcat caaagatttc taatgtttca ttatttccat gccaagtagc
    12241 ccactgaatt gtttcataaa caccaggatt agaaattata aaattaaagt atgctatact
    12301 tatacacttt atagctgtat ctccaaaatt tccaatagtc gcctttatca tttgactatt
    12361 catctctctc attcctttat gagctacttc aagtaataaa tcatctagac tttctatatg
    12421 attgtacaaa gatggtgtac gtattcctag cttttctgca ataactttta aagaaacttt
    12481 gttaagccca tctttattaa ctatatctga tgtagcttgt ataactattt ctctactaac
    12541 acgcatgatt ttccctcttt catttaaaat taattacttt attagtttag ctaatctaat
    12601 tagtttcgtc aattattgta atttataaaa ttctttatac tcttataaaa tcttgacagt
    12661 ttaattataa tttaatataa tactaattga attagtttta atactaatat aattagtatt
    12721 atattaaatt atatattaca acattatttt atagtttgat taattcaaca atcactaata
    12781 tatcaaactg aaaggagatt tttatggaaa aaaaatattt cgaaattcat ggagttcctt
    12841 caatcctttg gggaaatcct tcaagtaaat tatatattta catacatgga caatgcggca
    12901 ataaagagga ggctgaagct tttgctaata ttgcagttcc acatgagtat caagttttaa
    12961 gtgttgattt gcctgaacat ggagaacgta aaagagaaaa aaattcattt aacccatggc
    13021 atgttgttcc agaattagta tctattatgg aatatgcaaa gtgccactgg aatcaaattt
    13081 cgttatatgc aaatagcatt ggagcatggt ttagtatgtt aagctttgaa aaagagaact
    13141 taagagagtg tatttttgta tctccaattc tagatatgca acagctcatt tcaaatatga
    13201 tgctttgggc aaatgtatca gaagaacagc ttaaacatga actaattatc ccaacttcat
    13261 ttgggcatac tctttcatgg gaatatctac aatatgtaaa acaacatcct atcactcaat
    13321 ggaatgtacc aacaaaaata ttatatggta aacatgatga acttactgaa atcggtgttg
    13381 tagagaaatt tgtagaaaag tttaattgca accttactat tctggaaaat ggcaaacatt
    13441 ggtttaacac accacaagaa ttaaattcat ta
//
LOCUS       DAGQWR010000112        13242 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.112, whole genome shotgun sequence.
ACCESSION   DAGQWR010000112 DAGQWR010000000
VERSION     DAGQWR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.112"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            31..486
                     /locus_tag="MIW95_002852"
     CDS             31..486
                     /locus_tag="MIW95_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBY3152164.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            535..1119
                     /locus_tag="MIW95_002853"
     CDS             535..1119
                     /locus_tag="MIW95_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBY3152165.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSREQMDEVKLAMGFKL"
     gene            complement(1124..1447)
                     /locus_tag="MIW95_002854"
     CDS             complement(1124..1447)
                     /locus_tag="MIW95_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3152166.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLESL"
     gene            1599..2117
                     /locus_tag="MIW95_002855"
     CDS             1599..2117
                     /locus_tag="MIW95_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY3152167.1"
                     /translation="MNFVELTKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGRQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2293..2961)
                     /locus_tag="MIW95_002856"
     CDS             complement(2293..2961)
                     /locus_tag="MIW95_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152168.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3076..4491
                     /locus_tag="MIW95_002857"
     CDS             3076..4491
                     /locus_tag="MIW95_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY3152169.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNSKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQYVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4714..5172
                     /locus_tag="MIW95_002858"
     CDS             4714..5172
                     /locus_tag="MIW95_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3152170.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            5169..5753
                     /gene="clpP"
                     /locus_tag="MIW95_002859"
     CDS             5169..5753
                     /gene="clpP"
                     /locus_tag="MIW95_002859"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBY3152171.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6075..7151
                     /locus_tag="MIW95_002860"
     CDS             6075..7151
                     /locus_tag="MIW95_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY3152172.1"
                     /translation="MIAISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7218..9257
                     /gene="bclA3"
                     /locus_tag="MIW95_002861"
     CDS             7218..9257
                     /gene="bclA3"
                     /locus_tag="MIW95_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBY3152173.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGGTGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGGTGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGATGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGVTGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGPTGPTGA
                     SAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAF
                     SMPRDGTITSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPL
                     TGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            9426..10187
                     /locus_tag="MIW95_002862"
     CDS             9426..10187
                     /locus_tag="MIW95_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBY3152174.1"
                     /translation="MKNKIETSMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            10496..10774
                     /locus_tag="MIW95_002863"
     CDS             10496..10774
                     /locus_tag="MIW95_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152175.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGEYIYIPRKSNNKKEWGSSTS
                     TREDLSIRNMQIYEDYQVGYDLKYLSEKYYLSLKSIQRIVLQEKRKDS"
     gene            11067..11642
                     /locus_tag="MIW95_002864"
     CDS             11067..11642
                     /locus_tag="MIW95_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBY3152176.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPETLEQFHTNDYNLAKQYAMNTELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            12080..12790
                     /locus_tag="MIW95_002865"
     CDS             12080..12790
                     /locus_tag="MIW95_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3152177.1"
                     /translation="MISIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSSGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            12784..>13242
                     /locus_tag="MIW95_002866"
     CDS             12784..>13242
                     /locus_tag="MIW95_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY3152178.1"
                     /translation="MLTNLSIGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIFIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMI
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKL"
BASE COUNT         5110 a         1609 c         2402 g         4121 t
ORIGIN      
        1 tgtattaaat aattaaggag gtgagtagct ctggatgaaa tagatttaaa aataattaat
       61 tctctaaaaa agaatagtag ggcttcaacc tctgaaataa gcagacaaat taatttatca
      121 gtaccagcag tctcagagcg tataaggaaa attgagcagt caaatttaat tcaaaagtac
      181 actattcaaa ttaatcggga aaaaacaaaa tataagcttt tggcttttat atttatcaat
      241 actgatttat ctaaaagtat gaaagaattt aagaaaacta tattggaata tgatactgta
      301 ttagaatgtc atcatatttc aggagagtat gattacttaa taaaagtatt agcagaagat
      361 acaagtaatc ttgaagagtt tatatcagtg actttgaagg aaattggggg gattcaaaaa
      421 gctaatacaa ccattgtttt atctacaatt aaagaagaat ttaatacaat aaaagatatt
      481 ttttaaataa tttactagta aagtgttaat tatattttag gaggaaaata gaatatgaaa
      541 gttttagttt ttttagcaaa aggttttgaa actatggaat ttagtgtatt tgtagatgtt
      601 atgggatggg ctcgtaacga ttatgggcat gatattgatg tagtgacttg tggatttaaa
      661 aaacaagtta tgagtacatt taatatacaa gtattagttg ataagacgat agaagaagta
      721 tgtgttgatg attatgatgc cctagctatt cctggaggat ttgaagaatt tggattttat
      781 gatgaagcat atgattcatc ttttttaaat ctaataaggg agtttaattc aaaagaaaaa
      841 ataatcgctt ctatttgtgt ggcagcattg ccagttggaa aaagtggtgt tttaaaaaat
      901 cgtaaagcta ctacatatca tttgaaaaat ggtaaaagac aaagacagtt aagtgagttt
      961 gatgtaaatg tagttaatga accaattgtt gtagataaaa atattataac ctcatattgt
     1021 ccagaaacag ctccacatgt agcttttaag cttttagaaa tgttgacatc tagagaacaa
     1081 atggatgaag ttaaattagc aatgggattt aaactgtaat gctctataga ctttctaagt
     1141 aatcagaacc ccagtcacac atggaatcta aaattggaat aatactcttt ccaaaatcag
     1201 atagcgagta ttctactttt ggaggaacaa caggataaac cactcttata attaaaccat
     1261 ctgattctaa ttcacgtaat tgttgagtta acatcttagg cgttgcttta ggaattaatt
     1321 tttttaattc attaaacctc aatatagtgt ctttaagatg ccaaagtata agtgtcttat
     1381 atttaccacc aatcaatgca agtgttgctt ctattgggca actgtaatta tctaaacatt
     1441 ttttcatttt tgagctcctt tatttatttt aaagtatctt tttagatact atattacaaa
     1501 aaagtgcatt attgtaatat agtaccatag tgataaaatt atagcatagg ttaaataaat
     1561 catcaactta gcctaataaa acaatagggg gtaaataaat gaatttcgtt gaacttacaa
     1621 aaaaaagata ttcttgccgt aattatcaag atagaaaggt tgaaaaagaa aaacttgaga
     1681 aggttttaga tgttgcaaga atagctccaa ctggtggtaa tcgtcaacct caaagattga
     1741 ttgttattca ggaaaaagaa ggtataaata aactttctaa ggctgccaat atatatgatg
     1801 ctccattagc aattcttgta tgtggagata aagacaaagt ttggacaaga ccttttgatg
     1861 ggagacaact tacagatatt gacacaagta tagttacaga ccatatgatg ttacaggcaa
     1921 ctgaacttgg attggcaagt gtttgggtgt gctattttaa tccagatata attagagaag
     1981 agtttagttt gccagataat ctagaaccaa taaatatatt attgatggga tatgaatcta
     2041 agataccaga gagcccagag agacatgaaa aaactcgtgt tcctttaagt gaaattgttt
     2101 cttatgaaac tctttaatta taaaattaag ttattcatag gataaatgta ataaagtgtt
     2161 tattataaac aaaattcaaa ataaataata tctacatata attaatttta gtgaagtata
     2221 tctagtttaa ctagaatgta tacttcacta atttatttat tcaaaatatt tatgaggatt
     2281 ttaatatatt aactaaaata tatcaattga attaactttt aaatctggaa aagcacttga
     2341 aatatagtgt tttatgtcat ttaaatggtc ttgaatttgc ttttctgata ttttatcagg
     2401 tccatataca aaatacataa tatttttagt atcttttgtt atagctgttc gattatcagg
     2461 gccattaaga atactagata atataccaat attatctgat aaaaataaat catcttttgt
     2521 taaaatatgc tctttaccac ctataccata aaaatgttca cttccatttg ctaattctat
     2581 tttcaaaggt aattccattt tatcaaggtc atgacctgct gttagaagaa gatttttcaa
     2641 ttctgctaaa aacattgact caattgttac acctacatct gggatacctt ttgattttaa
     2701 tagaatagat tctaattgaa gcaatacagg gtaagttttc ttgaattttt tataatactt
     2761 tatataactt gaaacaggct ctgttttaat tttttcttta cgattgtatt ctgggtgttg
     2821 ttctataata tttttaattg tagagttttt aagaactaaa aaattttctt tattaggttt
     2881 tgaatgtgta atatttataa ccataactcc aaatttaata ttgggataaa tttctttaac
     2941 tttatgtgat atttctaaca taatatagac tcctttttat tttatatttt atgatataat
     3001 cttagcaagt taaaagtgga ttttgtagtt ccatttttat agtaatttta tagaaccact
     3061 tttggaggta ttagaatgtg gattacgatt aatagaaata gtaatatatc attatcaaga
     3121 caaatttatt gtgagataaa agatatgatt ctggatggaa gcttaaagtt tgagcaaaaa
     3181 ctgccatcat ctaggacttt atcaaaagaa ctatctgtat ctcgtaatac agttttagat
     3241 gcatataatc aattaattgc agaaggatat ttggaaacaa ggaatggata tggaacaatt
     3301 gtagctaaag gaatatcagg gcataaagtt gattttaagt taaatgatac ttttagagat
     3361 gaaaaagatt tacatactaa taaacaaaaa aattatatag attttcgttc aggagttcca
     3421 gaattaaaac tatttccacg caaagaactt tcaaagctat ataaacaaat actgtatgat
     3481 ttatcagatt cggatttcag atatggaagt acagctggtg tatgggtttt aagagtggaa
     3541 atatctaagt atttatttcg tactagagga attagatgta attccaaaaa cattatgatt
     3601 gtttctggtt caacacaagg tttatcttta atttcacagc ttttatacaa aagtaataaa
     3661 aaggttgctg tagaagaccc tattcataaa ggcttattaa aggttatttc atctattgga
     3721 tattcagttg ttggaattaa agtagatgat aagggaatga ataccaactt aataaaatca
     3781 ataaagggga tttcttttgt ttatactaca ccttctcatc aatatccatt aggagggatt
     3841 ttacctattc aaagaagact agagcttatt aaatgtgcaa cagagaatga ttactatata
     3901 attgaagatg attatgatag tgaatttaga tatgagggtc aaccaattag ttctctttat
     3961 gaacttagcc caaataaagt aatttacata ggttctttta gtaaaatatt atctccagct
     4021 attcgtgttg gatttatgtt attaccagac aatattttag aaagctataa aaccttaaaa
     4081 atgtactctg atgttcatac agaaacaata ttgcaatatg tattagctga atttattgga
     4141 aatggaggtt tagagaagta catatttaga atgaagaagt tgtacaatag aaaacgtaaa
     4201 tatctcataa aagttttaaa cacggaattt aaaaatgaat ttaaaataaa aggtgaagca
     4261 gcagggcttc atatagttgc tcatttctat aatgttaaat ttacggaaag tttaattcat
     4321 aaaatttttg aagcaggtat aaaagtttat cctatacaac aatatgcaat tgagaatata
     4381 ttagaatatg accatgaaat tatactaggt tatgctcatc ttagttttga tgaaattgaa
     4441 aaaggtgtaa aaatactcag caaaataata catatgaatc aaggtacata gataaaacat
     4501 gtagcaatct acatataata gatgtaatta agtttaaact ttaaaagaaa tatatattaa
     4561 tatttttgta taaaaatact tgatattgtt ttatcgatac tttattgcta tacactaaat
     4621 taagatagca atttatatta agaaaaaact ttttgcatag gttataatac ttaaaaggag
     4681 aggatagagt tgaaaacaaa tacagatgtt attatggaga taattgatta tatagaagaa
     4741 aatcttacag aaaatctaaa tttagataaa atagcttata ttgctaatta ttcaaaatac
     4801 catttacaca gaatgtttac aaatgttgta ggatgtacta tccatcaata catacagagg
     4861 aggagactta ctgaagcagc tagacaactt atatttacag acaaatatat tatagatata
     4921 gctattacag taggatattc tacacatcaa tcttttacac tagcatttag aaatttatat
     4981 aaagaatctc cacaagtata tagaaataaa gatgaatttt atcctattca acagagattt
     5041 gttttaaatc taaatcatca aattcaaagc gaaaaatgta tttcttatgc aaatatgaat
     5101 tataaacaaa gaagagatga aatcaattct tatcttaact ttaatattaa ttgtgaggtg
     5161 aaagtagcat gagtttagtg ccacatgtaa ttgaacaaac aggtcaaggg gaaaggtctt
     5221 atgatatata ttcaaaatta ttaaaagata gaattatatt tataggagaa gaaattaatg
     5281 atgctattgc aagtttagta gtagctcaat tactgttttt agaatcagaa gacccagatt
     5341 cagatataat tatatatata aatagccctg gtggctctgc tacatcagga tttgctatat
     5401 atgatactat gaattatgtc agatgtgatg tatcgactat ctgtattggt atggctgcaa
     5461 gtatgagtgc ttttttactt gcaggaggaa cacatggaaa aagatgtgct ttacccaatt
     5521 cagagattat gattcatcag ccaatgggag gagctaaagg tcaagcaaca gatgtaaaaa
     5581 tagctgtaga taatatattg aaaataaaag aacgattaga taaaattctt tctgaaaata
     5641 caggtaaatc aatagaagat attagaagag atacagatag agataatttt atgactgcac
     5701 tagaggctaa agagtacggg ctgattgatt atataatgaa taggaatgaa taggaatgaa
     5761 taaagtatca aagtatcata ttaaaatgtc tgttttacaa atatatatat aatattaaat
     5821 aagagtgtct caaaatgaac tttttagttt atgggacact cttttttgta tgaaattaaa
     5881 tatggtttta tcatttcaac aattattacc aaatttcaag tttaataaat tctactataa
     5941 aaaatacata aaagataagg ttctttagta actttctgtc gtcaaaagtt tttataaatt
     6001 taataacaaa acaagagaaa ataccatata ttacttatgt ctgcaatata tagaagacaa
     6061 aggagttgta cattatgatt gcaataagtt tgtgcatgat tgttaagaat gaggaagaag
     6121 ttataggcag atgcttagaa tgtgtaaagg atatagtaga cgaaattata attgtagata
     6181 ccggttctac agattccaca aaagaaatag tctcaaaata tacagacatg gtgtacgatt
     6241 tcgaatggat agatgatttc tctgctgcaa gaaatttttc attttctaaa gcttctaaag
     6301 actatactat gtggcttgat gcagatgaca taattttaga agctgataga gaaaaattat
     6361 taaagattaa agaaagcttg gatacttcca ttgatattgt tatggcaaaa tacaatgtat
     6421 cctttgatga aaatggtaac ccaactctct catattatag agaacgattg tttaaaagaa
     6481 gttctaatca taaatgggtt gaccctattc atgaagtagt acctcttttt ggagaggtat
     6541 tttattctga cattgcaata tctcataaaa agcttcatag acaggaccct ctaagaaatc
     6601 ttagaatttt tgaaaaaatg atttcagaag gaaaaacatt ggagccaaga caccaattct
     6661 attactctag agagttgtac tataatgcta ggtataaaga agcaatagaa ggttttacta
     6721 agtttttaga ctcatcaaga ggatggattg aggattgtat cagtgcctgc agagatttag
     6781 ccacatgtta ttacttaatt aatgatgaaa aaagtgcctt atattcattg tttagaagtt
     6841 tcgaatttga tgagccaaga gcagaaattt gttgtgacat aggaaagcat atgtttgata
     6901 gacaaaaata taaagaagcc attttttggt ataaggtagc attaacaaga gataagaatg
     6961 atactaatgg aggttttaag tcaaatgatt gttatggata tataccatct atacaattaa
     7021 gtgtctgtta tgacaggtta ggagaatcag ataaagctat ttattatcat gaaaaaacaa
     7081 aagaaatcaa acctaacgat agtgctgttt tgcacaatga aaattatttt agtaaattta
     7141 aaaatagttg ataggtcaca atcatactaa atttgacata ttatatatag agcttaaaag
     7201 ctcaaatata tcaggaggtg cttttaataa tgagtagaaa taaatatttt ggaccatttg
     7261 atgataatga ttacaacaat ggctatgata aatatgatga ttgtaataat ggtcgtgatg
     7321 attataatag ctgtgattgc catcattgct gtccaccatc atgtgtaggt ccaacaggcc
     7381 caatgggtcc aagaggtaga accggcccaa caggaccaac gggtccaaca ggtccaggag
     7441 tagggggaac aggcccaaca ggaccaaccg gtccgactgg cccaacagga aatacaggga
     7501 atacaggagc aacaggatta agaggtccaa caggagcaac agggggaaca ggcccaacag
     7561 gagcgacagg agctataggg tttggagtaa caggcccaac aggcccaaca ggagcgacag
     7621 gagcaacagg agcagatgga gtaacaggtc caacaggtcc aacgggagca acaggagcag
     7681 atggaataac aggtccaaca ggagcaacag gggcaacagg atttggagta acaggtccaa
     7741 caggcccaac aggagcaaca ggagtaggag taacaggagc aacaggatta ataggtccaa
     7801 caggagcgac aggaacacct ggagcaacag gtccaacagg ggcaatagga gcaacaggaa
     7861 taggaataac aggtccaaca ggagcaacag gagcaacagg ggcagatgga gcaacaggag
     7921 taacaggccc aacaggccca acaggggcaa caggagcaga tggagtaaca ggcccaacag
     7981 gagcaacagg agcaacagga ataggaataa caggcccaac aggggcaaca ggagcaacag
     8041 gaataggaat aacaggagca acagggttaa taggtccaac cggagcaacc ggagcaaccg
     8101 gagcaacagg cccaacagga gtaacagggg caacaggagc agcaggacta ataggaccaa
     8161 ccggggcaac aggagtaacc ggagcagatg gagcaacagg agcgacaggg gcaaccggag
     8221 caacaggtcc aacaggagca gatggattag taggtccaac aggagcaaca ggggcaacag
     8281 gagcagatgg attagtaggc ccaacaggtc caacaggggc aaccggagta ggaataactg
     8341 gagcaaccgg agcaacagga gcgacaggtc caacaggagc agatggatta gtaggtccaa
     8401 ccggagcgac gggagcaaca ggagcagatg gagtagcagg tccaaccgga gcaacagggg
     8461 caacaggaaa tacaggagca gatggagcaa caggtccaac aggggcaaca ggtccaacag
     8521 gagcagacgg attagtaggt ccaacaggag caaccggagc aacaggatta gcaggagcaa
     8581 ccggagcaac aggcccaata ggagcaacag gtccaacagg agcagatgga gcaacagggg
     8641 caaccggagc aacaggtcca acaggggcag atggattagt aggtccaacc ggagcaacgg
     8701 gagcaacagg ggcaacaggt ccaacaggcc caacaggtgc tagtgcaata ataccttttg
     8761 catcaggtat accactatca cttacaacta tagctggagg attagtaggt acaccaggtt
     8821 ttgttggctt tggtagttca gctccaggat taagtatagt tggtggagta atagacctta
     8881 caaacgcagc agggacattg actaactttg cattttcaat gccaagagat ggaacaataa
     8941 catctatttc agcatacttc agtacaacag cagcactttc acttgttggt tcaacaatta
     9001 caattacagc aacactttac caatctactg caccaaataa ctcatttaca gctgtaccag
     9061 gagcgacagt tacactagct ccaccactta caggtatatt atcagttggt tcaatttcta
     9121 gtggaattgt aacaggatta aatatagcag caacagcaga aactcgattc ttactagtat
     9181 ttactgcaac agcttcaggt ctttcattag ttaatactgt agcaggatat gcaagtgcag
     9241 gaattgcaat aaattagatt agataagaaa aggcttctaa tatcgtattg atattaggag
     9301 cttttttaag aatgattgat gaacatagtt ttaatattaa ttagtgcgca tattataaaa
     9361 tactattata taatataaaa taataacatt aaattggaaa tatagaatta tctaggggtg
     9421 atactatgaa aaataagatt gaaacatcaa tgaacaactt tgtaaaaaat tatcataaat
     9481 caaataatat aactacaaag tggcaagaac ttttgtttgc ttatgcatct gctgaagatg
     9541 atatgtttca gaagctaaaa agtgttgtag gaacatctca tgctatgcca aaagattttc
     9601 tggaaaatgg aaaaactata attacatatt ttataccttt tgatgaaagt gtaaataata
     9661 gtaatattgg agggtttgaa tgttctgaaa tatgggcaag agcgtacata gaaactaata
     9721 tgttgatttc aaatttaaat gatttcttat gtgaagaact agagaagatg ggggctaatt
     9781 catatgctat tcctgggtcg tataattttg acagtgaaaa attggtaagt gactggtcac
     9841 aaagacatgt agcattcata gcaggtcttg gaacatttgg tttaaataat atgcttatta
     9901 cagaaaaagg gtgttgtgga agaataggaa gcataatcac aagtttagaa ttagagccaa
     9961 ctaaaagacc agaaaatgaa tactgcttgt ataaacataa taatagttgc aaaaaatgtg
    10021 tcaacaaatg cgtgaaagaa gctttaaaag aagatggatt taataggaat aagtgttatg
    10081 aaatgtgtat gataaatgaa aaaaaataca cttatatagg tggttgtgat atttgtggaa
    10141 agtgtatagt tggggttcct tgttcggtaa taaatccctc aagataaagg acttatgttg
    10201 attaataact ttaaatatat ctaataagaa gaaaaaagtt agtctctaaa atagatgaat
    10261 tatgaaaata aataaagata atataaaggt tagatgaaga agttatgtta attgacaact
    10321 atttatactg ctgatagact tatatgtatc gttgcaatat ataaggaggg gtgagattta
    10381 tgtttgattt aatctttatt tttgcatagc aagggctatt gcaaatataa aatttaatat
    10441 ataaatagcc ctttgctatt cctaataaaa aattattatt taataggagt gcagaatgag
    10501 ctataaaaaa gcaatacata tattaccaga agaattactt gaattgatac aggaatatgt
    10561 agatggagaa tatatctata ttcctagaaa gtctaataat aaaaaagaat ggggaagtag
    10621 tacgtctact cgtgaagatt tatcaattag aaatatgcaa atttatgaag attatcaagt
    10681 tggatatgat ttgaaatatt tatcagaaaa atattacttg tctttgaaaa gtattcaaag
    10741 aattgtatta caagaaaaaa gaaaagatag ttaagaatga gccagtatag ttttataatt
    10801 atactggctc attttctttt gataaaagtg tttgaggtat agtttcttat aagtggatgc
    10861 tataatttaa ataatttcaa taacttgtac agcagataac gaatagagtt gtaaagtatc
    10921 taataagaag tagtagcttt ttatatgttt gaaattaaaa tttattaaaa ttaggaggtc
    10981 ttgttttgaa aaaaatactt gattatattc caccttttca taactttcta ttaatttagt
    11041 aatcttatag aaaggaagtt gcatttatga aaaaaattac ttgggaggtg gaatatatct
    11101 cccagttacc tgtaattagg tattgtaaga aatgtggaaa aaagactgaa tatatttgtt
    11161 caggtttatt tagggttaat gctcaacata aatatttgga tatatggtta atatacaagt
    11221 gttcaaattg tgattcaaca tggaactcaa ctatatattc tcgtgtcaat ccaaaaagct
    11281 taaatccaga aacactagaa caattccata caaacgatta taatcttgct aagcaatatg
    11341 ctatgaatac ggaactttta catagaaatg gagcagaagt tggtattcca gagtataaaa
    11401 tagtaggaca agaaattaat attaatgacc ttacacaagt tcatattatt agtaaatatc
    11461 catgtcagtt aaaggtatca acattattac gtgagaaact aggtttatct aggaaggtat
    11521 ttgacgaaat gttggattgt gggattatta gaagtaactc aggagttgat ttaaagaaag
    11581 gtaaattaaa ttatgaaatt atgttagata taggcaagga gaagcaaaag tcttcaaatt
    11641 gattagaata actaacttaa tatattttca ggaacatcat ttaaaaaaat agtggcttta
    11701 aaaaatttaa agccactatt ttttaatatg ttttatatga atatttaaaa tatagtgtgt
    11761 attcttcaaa attatagcga taattaaaat attactataa aaaggaaggt gcatactttt
    11821 tgtttaacaa aaactagata ctaaaaactt ctcgaagaat atgttattaa aatcgctaaa
    11881 tatattcaat atatattaaa aaattgtatt ataatgaaaa ttaaaataga ttgtaaatcc
    11941 aaaaagtaat acttaatgtc caaaaagcta aaattaaaat ctaaatagct atttgctaca
    12001 gaagtatgtt aaaatatgta aaatgaatag aaattttata tatttattaa aaatatataa
    12061 gaaaggatgt ttaagaattg tgattagtat aggaatatgt gatgatgaac tacattatcg
    12121 aataaaaata aaagatatat taagtgaaat attaagttca tatcctataa attataacat
    12181 atatgaattt tcatcaggag aagagttact aaataactat cctaaagatt tagacatatt
    12241 aattatggac atacaaatga aaacaataaa tggtatggat actgctagga agataagaga
    12301 gtttgatcat aagttagaaa taatatttgt tacttcattt gtagagttta tgcaagaagg
    12361 ttatgaggta aaagcataca gatatatact aaagccaata aataaagaaa aaatttctaa
    12421 aagtgtatta ccttgtataa atgagatgat gaaaaagaga aataattact taactataaa
    12481 tgtaaaaaat tatgtagacc gaattaaaat agattctatt acttatatag aaacagatag
    12541 accgaatata ttgatttata cacatgatga tatgtatatt actaaaatga gtatatctaa
    12601 aatagaaaag atattaaatg aatatggatt ttttagatgt cataatagct atatagttaa
    12661 tttaaaatta gtagaatcaa tgagtggaag tactgttata gtagatggca aaagtattcc
    12721 aataagtaaa tatagagtta aaggcttgaa acttgctata actaatatat taggggatat
    12781 agtatgctaa caaatctaag tataggattt tggaatatta ttaatttagt ttcaggtact
    12841 atagattgga ttatattttt tttagtaatc cacttagttg gaaagaggaa aatatctaat
    12901 ataagatata tatttacaac aataattata tttatattta tggcatacat aaatgtagtt
    12961 aaagtgtttc caaatattaa gattcttcta tgtatggtaa taggaatatc attttatata
    13021 acatcatatg aagataaaat atataaaagt ataatagtta gtttattgtt ttggctagga
    13081 ttaatgattg ctgaaggttc tgcagtaggt ctagtagtat ttataaataa attaaataat
    13141 attaacataa cacaacatga aaacttattt agaatacaag ctattattat gtcaaaggta
    13201 tttttattta taggttttat attgtttaag tattttaaat ta
//
LOCUS       DAGQWR010000113        13155 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.113, whole genome shotgun sequence.
ACCESSION   DAGQWR010000113 DAGQWR010000000
VERSION     DAGQWR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13155
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.113"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            76..777
                     /locus_tag="MIW95_002867"
     CDS             76..777
                     /locus_tag="MIW95_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBY3152179.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIINIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1048..1866
                     /gene="larE"
                     /locus_tag="MIW95_002868"
     CDS             1048..1866
                     /gene="larE"
                     /locus_tag="MIW95_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBY3152180.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNMDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKILGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLFNLGFSQFRVRM
                     HGEIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            1921..3069
                     /locus_tag="MIW95_002869"
     CDS             1921..3069
                     /locus_tag="MIW95_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBY3152181.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3205..4593
                     /locus_tag="MIW95_002870"
     CDS             3205..4593
                     /locus_tag="MIW95_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152182.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYTTLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4622..5320
                     /locus_tag="MIW95_002871"
     CDS             4622..5320
                     /locus_tag="MIW95_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3152183.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     ENDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            5323..6756
                     /locus_tag="MIW95_002872"
     CDS             5323..6756
                     /locus_tag="MIW95_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3152184.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6778..7515
                     /gene="truA"
                     /locus_tag="MIW95_002873"
     CDS             6778..7515
                     /gene="truA"
                     /locus_tag="MIW95_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBY3152185.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    7626..7833
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8086..9285
                     /locus_tag="MIW95_002874"
     CDS             8086..9285
                     /locus_tag="MIW95_002874"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBY3152186.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9532..10578
                     /locus_tag="MIW95_002875"
     CDS             9532..10578
                     /locus_tag="MIW95_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBY3152187.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DGGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDERIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPSKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10686..11849
                     /locus_tag="MIW95_002876"
     CDS             10686..11849
                     /locus_tag="MIW95_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY3152188.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKIIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SKNPSSTDLQEIAKEITDISKKDYEIFSMYTGDDNWYYMIQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(11938..12318)
                     /locus_tag="MIW95_002877"
     CDS             complement(11938..12318)
                     /locus_tag="MIW95_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBY3152189.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(12321..12980)
                     /locus_tag="MIW95_002878"
     CDS             complement(12321..12980)
                     /locus_tag="MIW95_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBY3152190.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         5291 a         1303 c         2272 g         4289 t
ORIGIN      
        1 aatagtggtt ttaaaaatct gacaacaaaa ataaaacttt caaaaaatta aatctaaaaa
       61 gaataggaga taaatatgag tattgaagat ttatatgatt ttgaatgtgt tcctttgaac
      121 aatgagattg atattgatgt tttatttgaa aagatgatag aagatggaaa agaaaaagaa
      181 tatacaccaa ttatagtcat tgatgaaaaa gtagggctat taagaaaaaa tatagatatg
      241 attactaaag agtatggttc tcttgaaaac tatagagagt attgtttaac aaaatataat
      301 gaaattgatg taaatcaatt ttttgatttt aggaagagtg agatttcaga attattaaag
      361 gagctaattg atttagaaga tgatttttat gattataaac attttagacc aattataaat
      421 atagatatat ttcatgaata taacaaagtt tacatagcta aaattccaac aattaaacca
      481 tatgaagtat ttgcatacat acctattgga ggatttaatg attgtcctag agatgaagaa
      541 catatagcta ttgcaaaata ttggtatgaa aagcatggaa tctttccaat cgcaattgga
      601 tgtgatacag ttcaatattc tgtaaaaaat ttaactaaag atggtaaaaa atttgatgac
      661 ttatgtattg aattagtatt ttattgtgag gatattatta tacaagggta tgaaacctta
      721 aaggctctta aggatgtatt aaaacgctct acaatagtgc ttttttggtg ggattgatat
      781 tctatcattt agatagtatt aaattgttag tcaccaagaa tccacttact ttagttatgg
      841 ggagtgtgtc aaactaggtt tttatacaca gtgtagcata gtaatatgtt taaattacaa
      901 aggttaaaaa aatactttaa tatttttaat attataatta atagatttaa aggtaattat
      961 aatttataaa ataaatactt tatatgttat aatcattaag aatgtaaaaa aataatatat
     1021 agttttatag ttcatatttt tccagatatg gaggtaaatt ttgtgaagga aaaagaaaaa
     1081 ttagataaat taaaaaaaat gttacttgaa ttaggcagtg ttgtagttgc ttattctggt
     1141 ggagtagata gtaatttttt gcttaaggtc gcaaaagata cattaggtga aaatgttata
     1201 gctgtgacca ttcatgcaat gatgcattca agtagggaaa ttgaagaggc taaacaatat
     1261 actcaaaatt ttggcgttaa acatatcata ttaaatatag aaaactttga tttaaaagaa
     1321 tttaaagaaa atggtgttga tagatgttat cattgtaaaa aatatatatt ctctaaaata
     1381 aaagaggtag caaaagaaca taatataaag tacatagtag atgggacaaa tatggatgat
     1441 ttaggagact atagacctgg gcttaaggca ttgagtgaat taggagtgat tagtccatta
     1501 aaagatagta gtttaaaaaa agaagaaatt aggtcgcttt caaaaatatt aggattaaat
     1561 acttttaata aaccatcatt tgcatgtctt gcaagtagga ttccttatgg agttgagata
     1621 acagatgaaa atttaagaat agtagaaaag agtgaagaat atctattcaa tttaggtttt
     1681 tctcaattta gagtgagaat gcatggagag atagctagaa tagaagttgg gcaggaagaa
     1741 ctaggtaaat tttttgaaaa taacaatttt aataaagttg atactaaatt aaaaatattt
     1801 ggctttaaat atgttacact agatatgtca ggatataaaa tgggaagtat gaatttaaat
     1861 gtgtagatta ttttaaaaat aaataagttt tatagttaat ataaggaaac aggaggtttt
     1921 atgaaaaatt atacattaat atcgccatgt ttttttggaa tggaaaaaat gctggctaga
     1981 gagattacta atttaggata tgaaataata aaaacagaag atggaagaat aacatataaa
     2041 actgatgagt ttggaatagc aaagtcaaac atgtggttga gatgtgctga aagagtacat
     2101 cttaaaattg ctgaatttga ggcaaaaagc tttgatgaat tatttgaaaa tacaaaaaga
     2161 ataaactggt ctagatatat accttatgga gctcaatttc ctatatcaaa agcttcctct
     2221 ataaaatcaa aattatacag tacaccagat gtacaagcca tagtaaaaaa ggctatagta
     2281 gaaagcttaa agaaaagtta tttagaggat ggtttgctta aagaagataa ggagaaatac
     2341 cctatttttg tatttataca caaagataaa gtaaccattt ctatagatac aacaggagat
     2401 gctttgcata agagaggata tagagaaaag gcaaataaag ctccaataag agagacttta
     2461 gctgctgggc ttatttattt aactccctgg aaagcaggta gagttttagt agaccctatg
     2521 tgtggttctg gtactatatt gattgaagct gctatgattg gtataaatat ggcacctggc
     2581 ttaaatagag agtttatatc agaaaagtgg agaactcttg ataagaaaat atggtgggat
     2641 gtaagaaaag atgcttttaa taagatagat aatgaatcta aatttaagat ttatggatat
     2701 gatatagatg aagaatctat agatatagct agagaaaatg ctgaaatagc aggtgtagat
     2761 gaatatatag agtttaatgt aggagatgca actcaattta aaagtgaaga tgagtttgga
     2821 tttataatca ctaatcctcc ttacggagaa agattagaag ataaagacag tgttaaacag
     2881 ttgtataagg agcttggtta tgcatttaga aaattaaaaa attggtcgta ttatttgata
     2941 acttcttatg aagactttga atatgagttt gggcaaaaag cagacaaaaa aagaaaattg
     3001 tataatggta tgttaaaaac taactttttc caatatccgg gtcctaagcc tcctagaaat
     3061 aataaataag ttttacatga tttatttgta aaatataaaa ttttattaaa ataagtaaaa
     3121 cattatgatg ccttgaaacg tctaattatt gtgaggagat ttttgtgaac aaatttaaag
     3181 atttatagga ttaggaggag ttaaatgaaa gtgttaaagc ttattactgt agtagccctg
     3241 atgatgttat ttgttactgg atgtaatata aaaactgaat ctccagaaca attagcgaag
     3301 atacctgact atgatgatac aaaaaaagta ttgtatgatg gtattgacca gttactaaaa
     3361 ccagattcat ctatgatatt gccttctaat acaaaagaag taggaaaaat aaataaggta
     3421 gatttaaaca gcgatggaat tgatgaatta gtagtatttg aacaaaaaga agatttaagt
     3481 aataatgtga gtcaggtagg gtttgtaacc ttgagttaca atggagaaaa atacgtactt
     3541 ggagaccatt ttttagaaaa tggcgaatca atagaatatg ctaattttta tgatttggac
     3601 tcagatggat ataaggaagt tatcttattg gttaaatcca aagacaaaac gaatatgcat
     3661 atatataaag tagtagataa tgaaataaca aaaatatatg acttggatgc atcatggctt
     3721 caaaataaag aagattttaa tgacatgaaa gtaaaaattg gatatataga tggagatgac
     3781 aagttagaca tattgatgct tcatttgaat aataagacta atgaaatgtt tgcaagtgtt
     3841 gcaaattttg atgggaaaat gaaaatcaaa gattctgtta agtttgaaaa tgtaaaaaac
     3901 ttaagtgaat tatatacaac attaggtaat gttgcttcat ctgttggaaa ttcttctgca
     3961 gctataaaag gtattgtact tgatattcct atattgaaag ataataacta tattacgcag
     4021 atactttata tgaaagatgg gaaaattaac aaagctttta gtgattatga taaaactatt
     4081 actaaatctt attatatacc tgtagatgat attgacaaag ataagattat agaaatacct
     4141 atagttgcag gcagtacagg taataataaa aatacttata gttctaaatc atctgcaact
     4201 ataagttggt atagatggaa tggaaaagaa ggtgctgact caagtttaat atttacaagt
     4261 cagatatatt ataattataa atataatttt aaattattta ttccaaataa tttatttgat
     4321 aaaatacttg ttgaacagga atttgttgga gaaaaagcag tatttaaatt ttactatttt
     4381 gactattcag atcgtaagaa tttatttaat atagtagtag agagtaaaaa caaacttgaa
     4441 gatagaaaaa atacaggtac tcaaaactct attgtacttc aagagagcga tgaatatact
     4501 ttcttactag ttgtaaacaa tgcaaatgaa atggataagc tagatatgac tatagacgca
     4561 ttaaaagaat atttctcatt aatatatggt taatattata ctcaattgga ggttttattt
     4621 tatgaaggaa aagatactta tactagaaga tgaaattggt attagaagtt ttgttagtat
     4681 aaacttaaaa agagaaggct atgaaatagt tgaggcaggt actggaagag aagctataga
     4741 aaaaatgaca acagaaaatg atattacaat agctctttta gatgtgatgc ttccagatat
     4801 aagtggtata gaagtatgta aatttataag ggagaatttt gaccaagtag gtataataat
     4861 gcttactgct aaagctcaag aggatgataa aatagaagga tttatttcag gagcagatga
     4921 ttacatcata aaaccattta gtataaaaga gcttttagtc agggtttctg ctcttcttag
     4981 aagagtagca aaggatgatt caagtgttaa gtcgagtgaa atagtttctc caccatttat
     5041 acttgatata gacaagagaa aattatttaa aaatggaaag gaaattgagc ttacaccaac
     5101 tgaattttca atagtgaaat atctaatatc aaatgctaag caatcgttaa gtagagacca
     5161 gatattagac gaagtatggg gaactaacta tttatatgat tttaaaatag tagatgtaaa
     5221 tataagaagg ataagaaata aaatagaaga tgacccatca aagccaaaat atatacaaac
     5281 tatttggggt tatggatact gttttagaaa ggaagaataa tagtgttaca ttttgaaggt
     5341 ctgagaaaaa aagtaataaa aaactatttt ataataatta ttataatggt tactctgttt
     5401 gaaggattgt tcatgtttta catacaaaac tactattatg attctgtcaa acaactactt
     5461 gaatctgaaa taaaatatgc tgatgaatat aatgctatta caatggaaac tacaagtttt
     5521 gagaaaaaag taaaaaatat atttgataaa caaccattaa ctaaaaattc agaatttgga
     5581 atttcaataa tagataaaga taaaaatatt atattagacc aatatggttt taaaagtaaa
     5641 gaaaaagcaa actatgaaga tgttaacaat gcattaaaag atataaaaac taaaaattta
     5701 acaccttata cttatagaat accagataca ggggagcatg ttatgagtat ctccttacca
     5761 cttaaggtaa ataatataat agaaggtgtt gttagatata cagtatcatt agatgctatt
     5821 gataatgcaa tattaaagca agctacatgg cttatattag caggaatatt tatacttatt
     5881 atagctatac taataagtct taaatttgct gagactctta taaaaccatt acgagagcta
     5941 aagaaatttg caaatgaatt ggctgtagga aattataata taaaattaga aaagatgaag
     6001 attgtagatg atgagattgg tgatttagca cagacatttg aacacatggc acatgagatt
     6061 gacaaaagtg aaaaattaaa agaggaattt atatcatctg tatctcatga actaaggact
     6121 cctcttacat ctataaaggg gtggagtgaa accttaggtt atgaaagtat aacaagagaa
     6181 gaactagact taggtttagg cattatacaa gatgaaacag aaagacttat aaaactagtt
     6241 gaagaattat tagacttttc aaggctttct tcagacagga taaaacttca tgttgatata
     6301 gttgatgttg aaggtttaat tgtaggtgtt gtaaatcaac ttaaagttaa agcagctgaa
     6361 aaagatatat cacttttatt tgaatttgaa aatgaattta tagagaacat tcaaggagat
     6421 aaaaatagat taagacaggt tctcataaat ttaatacaaa attcatttaa atttacgagt
     6481 caaggtggtt acataaaagt tgtagcttct caagatgaag agataacaac catatcagta
     6541 gaggataatg gttcaggtat agaaaagcaa aatttaaata aagtgttaga taaatttttc
     6601 caagaagatt ataataaggc tggtagtgga ttaggattag caattagtaa tgaaattgta
     6661 aaacttcatg gtggaagaat gaaaatagaa agtgaaaaga atgttggaac aaaaataact
     6721 tttaatataa aaaataaatt tgctaaacaa gcataaattc ttagaaagag gattaagatg
     6781 agaaacataa aaatgatagt agcatacgat ggttcaagat ataaaggata tcaaaaatta
     6841 ggtgacaata atatgactat tcaagaaaaa cttgaaaatg ttttaagtaa aatgactaat
     6901 gaaacagtgg agataatagg gtctggaaga actgatatgg gagctcatgc aagaggacaa
     6961 gtggtaaatt ttagaactaa ttgtatggat tctttggata aaattcaaaa atatttatat
     7021 gaatatttac cagaagatat agttgtaaaa actgttgaag aagttgatga aagatttcat
     7081 tcaagataca atgtaaaatc aaagacttat atgtataaaa ttgacaataa taagtatcac
     7141 aatcctttta taagaaaata tgctactcat gtaagtaaaa aattagattt agatagaatg
     7201 agaaaagcaa gtgagtattt agtaggagaa catgatttta ctagttttgc ttcttctaaa
     7261 tctaagaaaa aatcaaatgt aagagaaatt tattctataa atataaaaga agatgataat
     7321 gttatagaaa tatatgtaga aggaaatgga tttttgtaca acatggtaag gataatagta
     7381 ggtgctttga tagatgttgg tctaaaaaga agagctccac aagatattaa gtatatgctt
     7441 gaatcaaaag atagatgtca atcatcagat actgcaccag ctaaaggact atgtctttgg
     7501 aaagtaacat attagtcaat tatataaaac tctctttaaa ataaaaataa aataaattat
     7561 atagttgaca taaatggaaa aataaactat tatatatttt ataagagaga tataaaactt
     7621 tatatttaaa tcctgtgaga aagagagtaa cttctatgtg ttttatagag agctgaggat
     7681 ggtggaagct cagcaaataa gtattagtga aaagaacttt ggaggatact aattgaattt
     7741 agtatgttag taggtaatct cgccttaaga gaaagagtgg ataagtttat cttatcaatt
     7801 agagtggtaa cgcggatata tttcgtctct tggtttttcc aagaggcgtt ttttaatata
     7861 tttaattatt agtgtttatc tggtatgttg aacatttact aattgattag ctaagtttat
     7921 caataagagt ggtaccgcgg atatttcgcc tcttagtatt ttaaaatact aaggggtttt
     7981 tttagtatta aaatgatttt atgtgagtct aaattctcag agtcattcaa tattaagaat
     8041 ttctataaaa taaaaattat aatttaagat tgggaggaat taaaaatgaa ggaaaaagta
     8101 gtattagcat attcaggagg acttgacaca tcaatcataa taccttggtt aaaggaaaat
     8161 tatgaagata tagatgttat agctgtttgt ggaaatgtag gtcaggaaga taaaatggaa
     8221 gatgtatatg aaaaagcttt acaaagtgga gcatcaaagg catatgtgga tgatataagt
     8281 gaagaatttg taactgaaac tatatttaaa gccgtaaaag cagaggctaa atatgaagga
     8341 aaatacttat taggaacttc tttagcaaga cctataatag ctaaaaaatt agttgaagtt
     8401 gctcataaag aaggtgcaaa atatatatgc catggatgta caggaaaagg taatgaccaa
     8461 gttagatttg aagctacaat tgcagctctt gacccgacta taaaagttat tgcaccttgg
     8521 agaatttggg atataaaatc aagagaagat gctatagact atgctgaaaa acataatata
     8581 aaggtaacag caactaaagc taaaatatat tcagtagatg ctaacctttg gcatgtttca
     8641 acagaaggtg gagatataga gcacctagaa aatgaacata aaaaagatgt ttataaacaa
     8701 tgtgtagacc cagaagatgc atgtgatgta gctgaatatg tagaagttta ctttgaaaaa
     8761 ggtgtaccta aaaaagtaaa tggcgaagaa ttatctccag ttgcactgat tcataagtta
     8821 aatgagttgg gttgtaaaca tggaattggt gttatagata tagttgagaa tagacttgtt
     8881 ggtatgaaat caagaggtat atatgagact ccaggaggaa caatccttta tgaagcacat
     8941 aatattttag aaagtgctac tttagacaaa gatacattac atttcaaaca aatggtatct
     9001 tataaatacg gagaacttat ttacaatgga ctatggtatt gtaaattaag agaatctata
     9061 gatgcattta tggaacaaac tcaagataat gttacaggaa cagtaaaagt taaactttac
     9121 aaaggaaata taaaacctgc tggaatattt actgaaaatg ctctttatga tgaaggaata
     9181 tcttcttttg gaaatagtga actttatgac cacaaagatg cagaaggatt tattaattta
     9241 ttcactttac cattaaaaat cagagctatg aaagctggaa aataataaaa taaattattt
     9301 aagagtgtat aaaaactatt tttaggtttt tatatgctct ttttttattt gaatttatta
     9361 aattatacag taaatagtat aaaaaaatat attatagttc caaatgactt aataatgtga
     9421 aaatataaga cctaaaatat aaattttata aatacaatag ttattgactc tctcattgta
     9481 agagggttta tactgaatct aatgaaaatt tatagtgagg agatagatta tatgagaaaa
     9541 ataattgaaa ttaaacatca ttcacatgat tatgagtgta tgtggaatgg tatagaagat
     9601 ttatatataa ataaaaccgg tgaatgcctt ccaaacgatt ttttcttttt actgtcaggt
     9661 tttggttcat tttgttatat gaaaacaaat aaagctgatt tgaaaagaat ggtagctttg
     9721 ggagatggaa ggacaaaaca gatgtataaa tttttagcac caattgttgg atttgaatac
     9781 aaacatcatg agtttaaaaa gtatgagcaa gcattaaaga aagcaaaatc tgaaattgat
     9841 ggtggatatc cagttatttt aggagcttta gatatgtatt atctaccata ttataagaag
     9901 ctatatcata aagaacacat tccttttcat tatatattaa tggtaggata tgatgatgaa
     9961 agaatatatc tgtatgattg tggtagaaca gaattattac atctttctta tgataaatta
    10021 cgtgaaagca tgaattgcaa ttcccctgga ttaagcaaag aaaatactat ttgcacaata
    10081 agaatgaata cttcaaagaa taaatataac attgctagag aagctattgc tataaagcgt
    10141 gatatgtttt taaatccatc taaaggattt ttgggatata aaggttttga aaagtttatt
    10201 aaagagttgc cacagtggaa cactgaattg gataaagatg attatgataa gattttgacc
    10261 aatatggtta tgttttttgg aacagtacca acaattccaa atgcaatcaa aggaattaaa
    10321 gaaccagata ctgttcaatt taaaggtgga tttgataaaa tgagtagaat gctcaacagt
    10381 attggggagg aatacaaaaa cgactctttt atacaagtag ctgatatttt tgataaaact
    10441 gctattataa ttgagaaaat atccaatatt ataatagatt atttaactca aacatgtgat
    10501 gatacagaac aacttccgtt attgttctct gaggtcttag agcatatgaa aacaggatat
    10561 ttaatcttgg atgtataaaa aatatattgt tgaccctaac attacgtaat agtgtaatat
    10621 gaaaatatag cttgaatata ttagtaggta ttgatggctg taaaattaaa atttgaggtg
    10681 tggatatgag aacagtgaaa caagtttctg atttaactgg aataagtgtg agggcattac
    10741 attattatga tgagataggt cttttaaaac caaataaaat tacagatgca ggatatagac
    10801 tttatgatga tgaatctatt aaaactcttc aacaaatttt attttttaag gagattgata
    10861 ttcctttaag agaggttaag gaaataatgt caagccaata ttttgataag gttgaagctc
    10921 taaaaaatca aaaaaaatta cttattttaa aacgaaagag actggatgaa ttgatagagc
    10981 ttataaatca aactctaaga ggagaaggaa atattgattt taaagagttt gatatgagcg
    11041 agtattttaa tgttttagaa gaatttaaaa gagaacatag gaataagata attaaaattt
    11101 atggtagtgt agaaaaatac aatgaatata tagaaagagt taagtctaat gaagagaaaa
    11161 ttgctaaaat ggctataaaa caatatggaa ctattgagaa gtttgcccaa gctataaaaa
    11221 caaattttag cagtgatata ttgaatttag gagaaaaatt tgatagatat aaaaatgatt
    11281 gtttaaaaga aaaacatcca aaattaaaag aattatatag aaagcttgta gaagatttaa
    11341 gtaagaatcc ttcttcaaca gaccttcaag aaatagcaaa agagataaca gatatatcta
    11401 aaaaagatta tgaaattttc tctatgtata ctggtgatga caactggtat tatatgattc
    11461 aaaattatct agtaaatcct atgtggatag aagaagtaga taaaaagtat ggaagtggag
    11521 caggcaagtt tattggacaa gtactaaaga cttatctaag agatagaaaa ccaaagataa
    11581 atacattgta tgaaaaactt gtagaagatt tgagtagaga ttgttcttca agggaagttc
    11641 aaagcattgt tgaagaaata gacaatgaaa tgaaaagaag taatgaattt tataaaatag
    11701 acaatggtga aagatacttt gattatatgt cagagcttta tttacaagat tctaactata
    11761 taaaagtaac tgataagaat tatggtgatg gtgcatctaa gtttattgga gaagctttta
    11821 agatttattt tgataataat aattgttgaa aatttggaag taaaaaatat gtaagtttta
    11881 taaaagcaca aaaataagga tgtagattag ctccatctat atccttattt aaagttatta
    11941 aaatatatag tttacatcaa acttaacact ttcaataata tttcccaaat atgtttttat
    12001 actttcaact tcttctaaag attttaattt attttcagta taaattgctt tgtccagaat
    12061 attaaacctt aatcctattt catcaaagta ctcatgattt aaaactttgt accaatggtt
    12121 tttttcttta ttccaactcg tagatattga atcagcttct ttttgagctt tttcccaatc
    12181 ttcattttct atagattttt ctaaaacaga tatattgtct ttatatcctt ctgtaaaatc
    12241 atatagttta ttgttaacgt aaaatccaaa taatacaaat aagatagtcc atagtataac
    12301 aaatgttgta gatttcaata ttaatcaccc tttttatttt gaataaatat tttgtcattt
    12361 tcatctaaaa cacaaataag aacatcttta aaagtttcta tgtgattaga ttttaaaata
    12421 cccattaacc agttagtatc tttttgagct atgtctagat ttttatttat tattctacca
    12481 tccagtataa gtgggatagg taaatgatta aaagctcttg gaggagtact attgtagctt
    12541 gatgctggta caacacttag attaccatca gtctccaaaa tagcatattg tacatctttt
    12601 aggttgaagt atccttgaat cctaagttgt tctaataatt catctatact aattctttct
    12661 tttttaagct ctttatatac aatttttcct ttatctatta atacagaagg tttacctgat
    12721 aaaaatgaac ttaattttct gctttttaat gataagaatg aaataagagt ctgcatgatt
    12781 acaagaccag atattgctgc aacaccattt atgattggta tattattatt ttccattggg
    12841 acagatgcaa cttctgcaat taatagtgtt ataactaaat caaaacagtt catttcagct
    12901 atttcacctt ttcccatgac tctaagtgca attagcactg ttatgtataa tataatactt
    12961 cttattaata ctactgtcat atctatcaat tactccttta aaaaatattt acacttagtt
    13021 agtatatcct atataaaaaa tttaattcaa agtataaaaa accttaaata attggattca
    13081 actatttaag gtttttttga taattttaat attaaatttt ttatggattt tctgttactt
    13141 ttatttattt taata
//
LOCUS       DAGQWR010000114        13080 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.114, whole genome shotgun sequence.
ACCESSION   DAGQWR010000114 DAGQWR010000000
VERSION     DAGQWR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.114"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            63..1418
                     /locus_tag="MIW95_002879"
     CDS             63..1418
                     /locus_tag="MIW95_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY3152191.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATVLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTIMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(1536..2204)
                     /locus_tag="MIW95_002880"
     CDS             complement(1536..2204)
                     /locus_tag="MIW95_002880"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBY3152192.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(2271..2699)
                     /locus_tag="MIW95_002881"
     CDS             complement(2271..2699)
                     /locus_tag="MIW95_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBY3152193.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            2806..3399
                     /locus_tag="MIW95_002882"
     CDS             2806..3399
                     /locus_tag="MIW95_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBY3152194.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENATKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            3519..4454
                     /locus_tag="MIW95_002883"
     CDS             3519..4454
                     /locus_tag="MIW95_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBY3152195.1"
                     /translation="MDKFKYAFDDKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(4725..5669)
                     /locus_tag="MIW95_002884"
     CDS             complement(4725..5669)
                     /locus_tag="MIW95_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBY3152196.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            5985..7415
                     /locus_tag="MIW95_002885"
     CDS             5985..7415
                     /locus_tag="MIW95_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3152197.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNADLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            7615..8199
                     /locus_tag="MIW95_002886"
     CDS             7615..8199
                     /locus_tag="MIW95_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBY3152198.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(8303..9082)
                     /locus_tag="MIW95_002887"
     CDS             complement(8303..9082)
                     /locus_tag="MIW95_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBY3152199.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(9152..10408)
                     /locus_tag="MIW95_002888"
     CDS             complement(9152..10408)
                     /locus_tag="MIW95_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBY3152200.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            10666..11565
                     /locus_tag="MIW95_002889"
     CDS             10666..11565
                     /locus_tag="MIW95_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBY3152201.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            11589..13037
                     /gene="ddlR"
                     /locus_tag="MIW95_002890"
     CDS             11589..13037
                     /gene="ddlR"
                     /locus_tag="MIW95_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBY3152202.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
BASE COUNT         4758 a         1882 c         1695 g         4745 t
ORIGIN      
        1 aaataaaatt attaattaat agtttcgtaa ttttataata gatttacagg gagtgataat
       61 aaatggatac aaaaatatct ataaacgaaa acatgagtct aggtaaaaga ttttttaaat
      121 acctagcacc atctgtagtt gcaatgtggg ttttctcact atatacaatg gttgatggaa
      181 tatttgtaag taaaggagtt ggagaattgg ctttagctgc tgtcaatata tcaatgcctt
      241 ttattaattt tatatttgca gtttctctat tattttcaac tggtgcatct accataattg
      301 caatctatct ggggaaaaaa gatattaaat ctgctaacga agtcttttca tttaacttag
      361 tatcaataat tatattatca ataattatat tagctataac tttttttaat ttagatagat
      421 tagcactatt tttaggagct acagaatcta caattggtat ggttaaagat tatttaggaa
      481 taataatatt ttttaacgga ttctttatag tgtcttattc tttagaagtt ataataaaaa
      541 ctgatggctt tccaatatta gctactgttg gtgtaataat atctgctctt acaaacatca
      601 tacttgatta cttgtttgta attgaatttg gatggggtgt aaaaggtgct ggtattgcaa
      661 ctgttttgtc tcaagtcttt tctactattt tcttcttaat acatttttta agaaaaaact
      721 ctactttgaa tttttcaaaa tttagaattg attttaagac acttagaaaa attgtattta
      781 taggttttcc tgattcaact actgaattaa gttgtggtat agttgtttta ctttttaact
      841 taagtcttac taaatatatt ggagaaaatg ctcttattta ttatagtgtc ataaattata
      901 taaatacatt agtacttatg actataatgg gtataactca aggtatgcaa ccacttacta
      961 gcttttatta tggtgcagga aatatagata atgtaaaaaa attattaaaa atggggataa
     1021 aagccacaat aatagcatca gttgctgtat ttgcaatttg tatggctttt tcaggaccaa
     1081 tagtctcttt atttatacac ccagaagaaa caatgctatt caatgaagga gttcgtgtat
     1141 ttaaaatatt ctctatatct ttcttattgg taggaattaa tgtaataatt tctggattct
     1201 ttgtatcagt agaaaaacca tcaatttcta ctgttatatc tcttggtaga gggcttgtaa
     1261 tagttgtttt aagtctaatt tcaatgatac taatatttgg tggacaagga atatggatga
     1321 ctactattgt ttctgagttt atatgtctta ttttatcatt agtttttcta aaaaagaatt
     1381 tttctacatt agactctaat ctaaataagg ttgcttagat gataagagta gcaatatatg
     1441 aaaatatcta aataaaaaat acctattata caatcttgca aatattagac ctttgcaaaa
     1501 attaggatta tataataggt attttattta tgtaattatt ttatttctaa atatccatag
     1561 ccatctttta ttaaagttcc ttttacttca tattctacag gttttttaaa tattggacca
     1621 tctacgacaa atgtatgata ctctccatgt tctccacatg ggtctgcacc agcatttttt
     1681 atcttctcca caagttctct agttaatttt tttcctaaaa attcctctcc taagtgcttt
     1741 aagtttactt ttgttacaac actacaaaat ccactgtcta taaattcata tactaaatcc
     1801 tctctactct cttgccataa tggtaaatct actttcatac caactgcttc acatctatca
     1861 gtatcccatt ttctatgact ttctatatct atatctccat atgcacatat cgttgctccc
     1921 atgtttttag cttttattaa agccctctca aagctctctt catatccttc tacaccacac
     1981 tcagcaacta ataaaggtaa tcctatattg ctacttactt gcttaagcat attatattct
     2041 aaattatgtg tccatgactt tccatgttct tcacttatag ttgtcaagag tgccacaggc
     2101 ttatatcctt ttttcaacat tcgatttaat gctaatatac tatccttacc accactaaat
     2161 gacattacaa atttttcatc agccaatttt aacacctctt tcatctaact ttgttttatt
     2221 aacataatta taccaaattt ttgagttgca tagcaaatta tattaaattg ttaattcact
     2281 ataaagaatg tccctaatat taaaagtata acccctatgt atttgtataa atgcattgtt
     2341 tcttgtccca aaattacgct atcaataaaa aatccaacta aaacttggga tacaagtgtt
     2401 aatataagtg tatttgatac tcctaaattt ggaattgcct ttactgtaaa atatattatt
     2461 aaacctccaa atactcctcc taataacaac ttataattaa aaccatttat agatactaga
     2521 cactttaagt cacctgtaac tattatactt gccaaaaaaa atattgctcc tacagttaaa
     2581 cttataaagg tggccactaa tgctgttgta ctttttccta gttctccatt tataaaagct
     2641 tcaagagctg tagaaccacc tgctaataat gcaaatatta ttgcccataa ttttgtcatg
     2701 gacaaacctc cttctatata attctattaa tagaagtcta tgatacaacc tattaattaa
     2761 ttactaagta tagtttaatt tttatacact aggctttatt tggatatgaa tttctgttat
     2821 aattgttcta tcgattttgg aggtgatttt atgtacacaa attttaatat cgattttaat
     2881 aactttaata aaaaagaaaa tgctactaaa tttatgttaa tgggagtatt gctcattatc
     2941 ttaggattat tatgccttac atttaagaca ttaggtataa aattaatttc ttggactttt
     3001 ggtattgcac ttttattctt tgcatattta aatttaaaaa atattaatga actaaaaaga
     3061 tatgctacta aagaagaaat taaaccctct attaatatcc aatgggttct aataattgca
     3121 tgtatattac tttttgtatt tcctcaaaaa atacaaagca tattttcatt atttttaggg
     3181 ttttatttaa tctttaatca actagtagca cttgtaaata gtaagaataa tccttattct
     3241 aaatttacaa cttggaatat agttaagatt ctatttggta tatgccttat attatctcca
     3301 ctttttttat ctagatttat agtatcgata atgtcttttt tcataatatt atttggacta
     3361 gttctatttt tctctggaaa tacagcgaga aaatattaat tattttctcg ctgtattttt
     3421 atattactta aaatagaatt tgtttaagtt atctatttgt ggtaatatgt tttttgtaca
     3481 agctcagcgt acaattttat tagaaatagg tgattttttt ggataaattt aaatatgctt
     3541 ttgatgataa aagatatcat acttggaatt actatcttag aaatactttt ggagaaaaag
     3601 tttttaaggt atctataaat gcaggattta cctgcccaaa cattgatgga agtcttggtt
     3661 atggcggatg tacctactgt agcaaagaag gctcaggtga ttttgctgga aaccctaaag
     3721 ataatcttat cagtcaattt tataatataa aagaaatgat gttaaaaaaa tggcctcatg
     3781 caaagtatat tggctacttt caagcctata caaatacgta tgctcctctt gaagttttaa
     3841 aagaaaaata tgagactata ttagaacttg aagatgtcat aggtctttca atatccacaa
     3901 gacctgactg tttgccagat gatgttgtag aatacttatc tgaactaaat aaaagaacca
     3961 atttatgggt tgaacttgga cttcaaacaa ttcatgattc tacatctaaa attataaata
     4021 gaggtcatga ttataaaaca tttgttgaag gtgttgaaaa attaaagtct aaaaatataa
     4081 aggttgtcgt acacattata aatggtcttc ctggtgaaga ttataatatg atgatggaaa
     4141 cagcaaaggc agttggtaaa atgggtgtag acggtataaa aatccatcta ttgcatgtta
     4201 taaaagatac tcctatggaa aagatgttac aaaatggtat gttaactctt atggaacaag
     4261 atgaatatat caatcttgtc tgtgaccaat tagaaatact tcctgaaact atgataatcc
     4321 atagactcac tggtgatggt aagagggatg aactagtagg tcctatatgg agcctaaaaa
     4381 aatgggaaat tttaaatcaa attgatgata ctttaaaggc tagaaattct tatcagggct
     4441 gtaagtttgt ttaatacaaa ataataacta tagaaatggt tatataaata tagtcctttc
     4501 tatagttatt tagtatttac aaaacaaaat attaatttgt acatttatac aatatataat
     4561 aaaaaataga tagtctaaac tgtatctact tatattttat ttgtactgtc cactttattc
     4621 tataaaattt ataaatagtt attttttaat accaaatata atttggtata atttttgttg
     4681 gattctaaaa tgtataccaa taaatccttt ttaagtatat aattctaaat actttcagtt
     4741 ttttcaatct gctttaactt gttggattta taaaatattc ttatttcatc ataagaaaga
     4801 ttaagtgcta ctactgcaga tattaacatc aacatcactc caaataactt ccctaaggaa
     4861 aaactttctc ctaatatagt gcaaccaatt attactgaac caactaactc tactgatgcc
     4921 acaacacctg caactgacaa ctctacacct ttagatatac ctgctgcata aaaaatatat
     4981 gcaagtgcag ctggaacaat gcctaacatt agcatacaag ataaaacatc taaatcctta
     5041 gcataattta agatttccca aggtctcgaa gaaggaatca taaatatagc tccaaataag
     5101 aaactgtata ctaatattgt tgaacttgaa aattccttta aagcattttt actaatagtt
     5161 ggcataagtg catatgctat tgctgataat accccaagta aaagtccaaa agcgtttaaa
     5221 ccttgaaaat ccaaacgtcc accagtcact gccataattg caccaataaa acaaagaata
     5281 agtgaaaata atttgcttct agtaatattt tctttataac atacttttga aaatattgct
     5341 aaaaacagtg gtgatgtata tagtaaaact gctgcaattg atacacctgc aatgtcaatt
     5401 gctttaaaat aacataaatt aaacattgcc tgacatataa ttcctattat tactgaatat
     5461 ataattcctt ttttgcttat ctttaagatt tgtggtgtcc ttatagatga atatacgcct
     5521 aaaactataa atccaaaaaa caatcttgta aatgctactt gttctgatgt cagtccattt
     5581 cccattaaaa actttccaaa taatccaagt gtagcccata ataaccctgc tattgctata
     5641 aatacatacc ctttactttt ttctcccatt ttaaattccc ttctattttt atttagtcaa
     5701 tttacactct tctttttaag caaaaattat gccatcttaa accaatatat atcgactcta
     5761 aaaccctagt ctttccaata atactaataa gttttcaatt ttcttatatt tatttttcgc
     5821 ttactttaag ttgatttaga cttattttaa ttgtaaaatg agttatatat aacttattta
     5881 aagttaaaat agactgaaac ttatataaat atcaaatttt aagtatatta atcaattaaa
     5941 cctattttaa tatatcatta aaaatttagg aggaaaatat aaaaatgaat ttagatttgg
     6001 aattttatca aaaaatatta gaagcttccc atgatgaaat atgtgtttct gatgataaag
     6061 gaataataat atattgtaat aaagcatttg aagaaaatta tggtctaaaa aaagaagata
     6121 ttttaggaaa aaatgtttct tttttagaag actctggata ttcaacaaaa agccccatac
     6181 caattgtatt aaaaacaaag actaaatttt cactggaaca agatactcaa acagggaaaa
     6241 agcttatcat aactgctacc cctatatttg atgaaaatgg aaacttagaa tttactgttg
     6301 aaaattgtag agatataact gaacttaata atataaaaaa taaacttgaa gacacaaaaa
     6361 agcaagtaaa aaaatataaa tctgaggtag aaacactata cagaactgca cttagaattg
     6421 aagatactgt aattatggac ggaatagtga tgagaccaat cataaacaca gtaaatcatg
     6481 tttctaaaac ggatgtaagt gtacttttat tgggtgaatc tggaacagga aaaagctcac
     6541 ttgctagata tatacaccac aacagcaata gatctaatgg cccatttata acaataaatt
     6601 gtgcaactat atctccacaa cttcttgaat ctgaactttt tggatatact tcaggtgctt
     6661 ttacaggtgc aagtactaaa ggtaaagttg ggctcgtaga attagctaat ggaggaactc
     6721 tttttttaga tgaaatagga gatataccac aaaatctaca ggcaaaattt ttgcaattaa
     6781 tacaggatag aacttttact ccagttggga gcttaaaaaa taaaaaggtt gatataagaa
     6841 ttatatctgc tacaaatgct gatttagttt caaaagtaaa agaaaaaaag tttagagaag
     6901 atttatacta tagattaaat gttatagaaa taaaattgcc tcctcttaga gaaagaaggg
     6961 ataatctagt ggaaattata aaatattatt ttaataggta ctcaagtgac ttcaacctaa
     7021 ataaaactat ttctaaagaa gctatggatg ctattgcaaa ttatagattc ccaggcaata
     7081 taagagaatt acaaaatata attcaaaaaa tcttgcttac ttgtacagat aatcatataa
     7141 caatagatga cttaccaaac attcttacaa aaaatataca tattaccaat aatggaaata
     7201 aaacacatat atcccaaatt aataaggtta ttatatctga ctcaaaatct actaattata
     7261 aaaataaaaa ttttgacacc ttaattaaag agtatgaaaa aaatataatt ttggatgctt
     7321 atgaaaaatt tggtagttct tataaagtag ccaaacacct tgaaatctca cagtctaaag
     7381 ccaataggtt aattagaaag tatactaata cttaatttta aatttgataa ataaaaactt
     7441 aacactattt acatattgta aatttggcca tttactgtta ttatgaatat ggttgaaatt
     7501 atttaaataa atactgttga cttagtttca tatatttacc attaaacttt tatcataaat
     7561 atcatatcat aataatatat ttaataagtc taataaacta tacgaggtgt aaatatgtta
     7621 atgaataccc attttattat ggcaaaatca atacttgata atatagatga aaataaaaca
     7681 ttctttataa gcgaaaaaaa ttttatatat ggcaatataa agcctgatgc tttttctaaa
     7741 tataaattaa aaaaacatta tttagatgag tcatttaata tgattgtttc taaaataaac
     7801 tatctatgca gtcttaatat agatagtctt tctaaatact tttcagtaag tagacttagt
     7861 caggaactag gagtcatatg tcatttttta tgtgattttt tctgtgtagc tcacagtgaa
     7921 cgttgggaat ttaagcatag cttaaataaa catgttactt atgaaagaga acttggagca
     7981 tttgcaaaag atgttgattt atctaaaatt aaatgtggta ctataaatag tagttttgag
     8041 aagtttttta ctaaacttta tagtgattac aaaaagaaaa ctgattatat gaatgattta
     8101 aaattttcag cttatacttg taatagtgtc ataaattata tacttgactc tattttatct
     8161 aatacaataa aatcatatca tatagcaaat tgcggttaac ttaagaattt ttaaatttat
     8221 ataaacttat aattgaatat aattaaaatg actgctctat agaatataat tttatacttc
     8281 tctaggacag tcatttttta tactaactat ttatctccat tataacatct atggcacttt
     8341 ttacactatc tactacataa tttgcacttt gctttacatc ttcccttgcc ttagacattg
     8401 caaaactata tttaaatcct tctaacatgc aaatatcatt tggagaatcc ccaatagtag
     8461 caacttcatc tggacttaca tttaacgttt ttattacatg ctctatactt cctctttttg
     8521 acacactttt aggaactata tttaaagtat atgttccaga aaaatatatt tctaatactt
     8581 cactaaactc actttttaga taaagttcta ttctggataa ctcttccaaa ttttcagaac
     8641 atacgcctat atttcctata agattatcaa aactacttct tttttcaaaa tctgggtcaa
     8701 ctataaagtc actagaataa tgtctaaaag cagtggtatc actacccaca attacatcac
     8761 ccttatattt aaaataaagt tgtgcatcac ttgattcaaa tctatcaatg acttttttta
     8821 ctaaattatc atctatcgtt cttttaaaaa ttacattata atctttatca tatataaatg
     8881 aaccattttc acatacataa tatccattca tatctaaact atctttgata atattatgtg
     8941 cttgtttaaa aattctacct gtagcaacat taaactgtac tccctttttt tctatttctt
     9001 caattgctac tatatcttct tttgttatgg ttccattttc atatagagtt ccatctaaat
     9061 cacaaaatat atgttttatc ataaattaaa agcctccaaa aacaatttta tattatagta
     9121 acacttcaaa tatatccaat atcataacaa cttaaagctt ttctaattgt attctcaatt
     9181 cattaagtaa atctctagta gctcttaatc ttatctttct tctatcccca ttaaatatat
     9241 ttttctttac tattgttttt ccatttatat atattgcagt ataaactagt cccacaggct
     9301 tctctttggt tcctccacca ggtccagcta ctccagttgt ggatatacct atattagctt
     9361 taagtccttt agcaacgccc tcagccattt ctatagctgt ttgctcactt actgctccaa
     9421 actcttccaa agtttctctt tttactccta atcttttcat tttagaatca ttagaatatg
     9481 ttacacatcc ttcaagaaat acagaagata ttcctggata gtttataaga gatgaagaaa
     9541 ccaaacctcc agtacaagat tcagccactg ctattgttag atttttttct acaagtaact
     9601 tagaaacagc atcttctaaa gcagtctccc cttcagatat ggaaatatct ccttcagtat
     9661 atacatattt accaactcta ctttttattt tttcttctac tggttttatt aatttaaaag
     9721 cttctctttc attttcagcc tttgcagtta ttcttattgt aacttctaat tcttttgcat
     9781 ataaagcaac tgttggatta gtctgctcat taattatatc gagaatttct aattctagat
     9841 ttgactcacc tattccatat aatcttaaag tcttagatac tagcatttca tttgtgaatt
     9901 gttgtaagta aggtttgaca ctctcattaa acatagcttt catttctctt ggaggtccag
     9961 gcaatactat tataaattta ccatcttttt ttaaaattgc ccctggagct gtaccattat
    10021 tattttttag tactatagca tcttttggaa agtacacttg tttcatatta ttttcggcca
    10081 ttttttttcc tgttttaaca aagaaagatt ccaaaacctc tagtgaaggt ttatgtagtt
    10141 ctaatttctg accaaagtat tctgcagcag tttcttttgt catatcatca ccagttggcc
    10201 caagaccacc tgtagtaatt acaaaatcac ttctctttaa gctttcatca aaacattcta
    10261 aaagtctttg tttgttatct ccaacagtac tctgatggta tacttctatt cccaatgatg
    10321 caagttcttt agctaaaaat tgagaatttg tatttactat atctcccaat aatatttctg
    10381 ttcctacact tattatttca gctttcaaaa tttatcactc cttgatttat agccattaag
    10441 actagtctta agttcataac aataatttta gcaaaaataa taacataaat aaagttaaaa
    10501 aatcatttaa acatatcttt aaactagatt attattctaa aaccacacat aatataaact
    10561 tcgtatatag tggtactttc atcctcaact gttaattcat ataattaagt atgtaaataa
    10621 aaagtttact ttaaaataat aaaaattaaa ataggagtgt ataatatgaa aatagcagtt
    10681 ataatgggtg gaatatcatc agagagagaa gtttctttaa attcaggaaa agagatatat
    10741 aataaccttg ataaaaataa atatgaagtt gtaaaagtaa taatagatga taaaaaagat
    10801 atatttacaa agataccaga agatatagat tttgcaatac ttgctttaca tggaaaattt
    10861 ggtgaagatg gatgtattca atcaatactt gagactatgg atataccata ctctggatgt
    10921 ggacctcttt gcagtgggat gtgtatggat aaaaatataa ctaaaaaaat gcttagagat
    10981 tcaaacctac ctactgctcc atgggtttta gtaaaatcag ttgatgaaat cgactatgat
    11041 gaaatagata atataggtta tccagtattt ataaaaccaa actcaggagg ttcaagtgta
    11101 gccacattct ttattcattc taaggatgaa gttgaagaag cagttagaaa aggtctggaa
    11161 gttgacgaat ttgtaatgat tgaaaaatat ataccaggtg gagaatatac ttcattcata
    11221 ttaaatggag aagtgttccc tacaatatct ataaaatctg attcaggatt ctttgattat
    11281 gaagctaaat actcagttga aaaaggtgca aaggaagaag ttgtttactt agatgaagaa
    11341 cttcaaaaaa gagtaaacga aatttctgag acttgttgga aaatttttaa ttgcaaagca
    11401 tatgtaagag ttgatatgat tataagtgaa ggtattccat atgttcttga actaaatact
    11461 cttcctggaa tgactcaaac tagtttaatt cctagaagtg ctgctgctag aggtattaag
    11521 tacagtgaac tattagataa acttatagaa tactctttaa attagatgaa ataatttgag
    11581 gtggtaattt gatattttca aatctaaaac taaatgataa tgaaccaatt tatatacaac
    11641 ttaaaaatta tataagtgat atgatatcaa aaggactaat tccagacaat agtaagctcc
    11701 cttctacaag ggagcttagt cagcttttac aagttagccg aaattctgta gttttaactt
    11761 atgaagaatt aaaatcagaa ggattaatct actctatttc aggaaaaggt acatttgtta
    11821 aatcaaagaa taagtcttca aatactacat ggtcattaaa ctgggattgt ttagagaata
    11881 catactctaa aaaagccaat gaattggaca ttataaaaag tgaaattcct tggagctctg
    11941 acctgatttc ctttaaaagt atttctccag atggagattt attcgacatg gaagaattaa
    12001 aaaaatcctt tttaaataga atttcactag aaggacataa attactcaac tatggatatg
    12061 cacaaggtta taagcccctt attgactatt tactagaata tatgacaaac aagggtgctg
    12121 atataagtaa taaagatata ctaataacaa atggattcac agaaggcttt gatattatta
    12181 tgtcatcttt tacccaagaa aaagactaca taatatgtga aaatcctact cataatactg
    12241 ctataaaaat tatgaaatct cataacctca atatcttagg tgtagatata aatgaagatg
    12301 gtttggactt caatatgcta gaagacaatt tagttaagta taaaaataaa ataaagtttg
    12361 catacattac accctcttat cacaatccta caggaattgt aatgactcct gaaaataggt
    12421 acaaattcta taatcttatg aaaaaataca atatagcaat tatagaagat ggatttaatg
    12481 aagaattact ctacaatagt tctcatatat ttcctatctg ttccctagat aatatgaata
    12541 atggggtcgt atatattggt agtttttcaa aaattttatt tcctggaatg agaattggat
    12601 ggatatttgc agataaaaaa gtaatcaata aactagaaag tgtaaaaagg tgcagaaaca
    12661 tacatgtatc ctttttagac caaggaattt tatttgatta tttaagtaat ggaggttttg
    12721 aaaaatacat taaaaaaata cgtaaattct atggggacaa atttaacttt gcatataaat
    12781 gtataaaaaa atatataaaa acagaatata tcttgggtga tggaggtttg catatattta
    12841 taaaactaaa aaatatagat actagagttt tattagaaaa atgttatgaa aaaaatgtta
    12901 tatttatgcc tggagattta ttttttacag ataactctgg ttttgatact ttaagactcg
    12961 gattttctag gttgtctttt gaagacattg aaattggaat aaaaataatt ggagaaacta
    13021 taaatgaaat gttctaggtt aactaaatat tgttagccta gtttattttt atgaggtata
//
LOCUS       DAGQWR010000115        12948 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.115, whole genome shotgun sequence.
ACCESSION   DAGQWR010000115 DAGQWR010000000
VERSION     DAGQWR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.115"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            136..2448
                     /locus_tag="MIW95_002891"
     CDS             136..2448
                     /locus_tag="MIW95_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY3152203.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKENNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDTTSNKVNYISDELD
                     TTSNKVNYVSDELDTTSNKVNYVSDELGMALHKVNCVSDELDMTSNKVNYVLDELDIT
                     SNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIKFKVCF
                     DMFMTDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFME
                     LAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNLVAWEDKKFET
                     PSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKK
                     SIAQAYINQRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHG
                     NPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            2536..2871
                     /gene="spoIIAA"
                     /locus_tag="MIW95_002892"
     CDS             2536..2871
                     /gene="spoIIAA"
                     /locus_tag="MIW95_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBY3152204.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            2885..3313
                     /locus_tag="MIW95_002893"
     CDS             2885..3313
                     /locus_tag="MIW95_002893"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBY3152205.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3336..4100
                     /gene="sigF"
                     /locus_tag="MIW95_002894"
     CDS             3336..4100
                     /gene="sigF"
                     /locus_tag="MIW95_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBY3152206.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            4380..4817
                     /gene="spoVAC"
                     /locus_tag="MIW95_002895"
     CDS             4380..4817
                     /gene="spoVAC"
                     /locus_tag="MIW95_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBY3152207.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            4971..5984
                     /gene="spoVAD"
                     /locus_tag="MIW95_002896"
     CDS             4971..5984
                     /gene="spoVAD"
                     /locus_tag="MIW95_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBY3152208.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            5998..6360
                     /gene="spoVAE"
                     /locus_tag="MIW95_002897"
     CDS             5998..6360
                     /gene="spoVAE"
                     /locus_tag="MIW95_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBY3152209.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            6543..7559
                     /locus_tag="MIW95_002898"
     CDS             6543..7559
                     /locus_tag="MIW95_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBY3152210.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFVGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDRSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            7934..8776
                     /locus_tag="MIW95_002899"
     CDS             7934..8776
                     /locus_tag="MIW95_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBY3152211.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            8791..9255
                     /locus_tag="MIW95_002900"
     CDS             8791..9255
                     /locus_tag="MIW95_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152212.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIITCFGQMIALKTQAQDLLIGSISG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            9242..10414
                     /locus_tag="MIW95_002901"
     CDS             9242..10414
                     /locus_tag="MIW95_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY3152213.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            10458..11393
                     /locus_tag="MIW95_002902"
     CDS             10458..11393
                     /locus_tag="MIW95_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBY3152214.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(12192..12590)
                     /locus_tag="MIW95_002903"
     CDS             complement(12192..12590)
                     /locus_tag="MIW95_002903"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBY3152215.1"
                     /translation="MKWKELYNNARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
BASE COUNT         4937 a         1541 c         2408 g         4062 t
ORIGIN      
        1 ctgtgacttt agtcatggga ggttcagaaa atatagctat gttttatata ctgtagtttt
       61 gaacatagaa agtttaataa attatagtat atattaattt ttaataaggt aagaaagtag
      121 aaagaaggat attttatgga tataataaaa aaattaagtc atggatgtac attagattgt
      181 catgattgtt gtaaattcaa tgtttatact aaagaaaata atgtagtaaa aattgaagga
      241 gataaaaatc atccttatac aaaaggtttt atatgcaaaa aagggatggc tcatttagat
      301 agattaaatc ataaagatag aataaagact cctatgctta aagttgatgg agtatggaaa
      361 gagatttctt ttgataaggc tatagaaata atggcagaaa agcttacata ctataaagaa
      421 aagtatacat caaagtctgt catgcattat gaccaatatg gaagtgggtc tgtgctaaaa
      481 tacataggag atatattttt taatttttat ggaggtgtta gcagacataa gggtggtccc
      541 tgttggagtg cagggatgca tgctcaaaaa tatgattttg gagtagctaa gagtcatgct
      601 atagaagata tgttaaatag taaaagtata tttgtgtggg gaaaaaatcc agcatatact
      661 acaattcata ctatgcaaat aattaaaaaa gctaaagaaa aagggataaa gatagtagtg
      721 attgacccca tatatacaaa aacagctcaa atagcagata agtatgtaca ggtaaatcct
      781 ggaacagatg gagctttagc tatagctatg gcaaaaatta tagtagaaga taaattatat
      841 gatgaagaat atataaactc gtatgttatt ggttttgaag aatataaaaa atatttatct
      901 tcattggaat taagtttttt aattgatgaa tgtggtgtta aagagaatga tataagagag
      961 ttagtggatt tatatactaa caaatattca agtataaatg ttggatatgg gcttcaaaaa
     1021 tataaaaatg gtggaaacac aataagagcg atagatgctt taggtgctat aactggtcaa
     1081 ataggattta gtggtggagg ggtaaactat gctaataaag tttatccaag tgttataaat
     1141 tctgaccctt acaatagcca atcatatggt gaagataggg agttttatgt aagtaatatc
     1201 agtaaattta tagaagaatc tttaaaaaat acatcaaata aagtaaatta tgcatcagat
     1261 gaacttgata caacatcaaa taaagtaaat tacatatcag atgaacttga tacaacatca
     1321 aataaagtaa attatgtatc agatgaactt gatacaacat caaataaagt aaactatgta
     1381 tcagatgaac ttggtatggc attacataaa gtaaattgtg tatcagatga acttgatatg
     1441 acatcaaata aagtaaatta tgtattagat gaacttgata taacatcaaa taaaacagat
     1501 tatatatcaa atgaattgta taatttatct aataagtcaa tcaaagataa tattcctata
     1561 aagatggctg tgataactaa aagtaatatg ctgaatcaac tgccagattt agtagaattg
     1621 gagagggtgt tttcaaaaat taaatttaaa gtatgttttg atatgtttat gactgataca
     1681 gctactttat gtgacatgtt tataccgtgt acaaatacac tagagagtga agatataatt
     1741 tatagctcaa tgaccaatcc atatataacc tataatgaaa gagctgtaaa gccagcacat
     1801 aaattgatgg atgaatatta tttctttatg gagctagcta aaaaaatggg attaaatgat
     1861 tatccttttg tagaaaagag aacttattta gaaaaagtga ttgaaccact taagcgtttt
     1921 gacaaaaatc tggatataga aaaacttaaa aacaattact ttactataca taaccttgtt
     1981 gcttgggaag ataagaagtt tgagactcca tctggaaaat acgagcttta ttcagaaagt
     2041 ataaaaaact tgggaataag tccaacacct gtatatataa gcaataaata taaagaaata
     2101 gaagataaaa atatttcttt tagattatta actaatcatc atgcagatac tttatttagt
     2161 cagcatttta tggacaaaaa atctatagct caagcatata taaatcagag aatggctaaa
     2221 aaagtaggta ttgaggacaa agatattgtg atattgaggt caaaaaaggc aaaaataaat
     2281 gtacaaataa atatagatga tggagtaggt aattatatag taaaaatgta tgttggttgg
     2341 tggaagaaac atggaaatcc taatagttta acagacactg gtatatcaga ttttggaggt
     2401 caggtaacat ataatgaaag tatggtagaa ataatacgac aaaactagtt aaatttcatt
     2461 gaaggaataa aaatataatt atagaattga ttaaaagtta ctatttttac aaaactattg
     2521 aaggagggat ttttgatggt aaattattcc ttagaacata agaatctata catagaattt
     2581 atgtgctcag aactagacca ccatgtagcc aatgaaataa gggaagaaat agataactta
     2641 ttaagtgtaa atcaagtaaa aaatgttgta tttaattttg aaaatattaa ttttatggat
     2701 tcttcaggta taggcgtcat cataggtaga tataaaaaga tttctaatga agggggacga
     2761 gtttcagtaa ttaatataag ttcacgagtt aagaagattt ttgatttatc tgggttaaat
     2821 aaaattattg gcatttatga tacgtatgaa gaagctctaa gttctttgta gggaggagta
     2881 tgtgatgaat aatattatgg aagttaaatt ttcggcaata tctgaaaatg aaagtttagc
     2941 aagagttata gtagcatcct ttgcagctaa gttagaccca acagtagatg agttagtaga
     3001 tataaaaact gctgtctctg aggctgttac gaatgctata atacatggat atgaagaaga
     3061 cagttctaag tttgttttct taagatgtga gatagaagga aatactataa agattatagt
     3121 tgaagatgaa gggtatggaa tagaaaatgt ggagaaggca atggaaccac tttatacatc
     3181 aaaaccagaa cttgatagat caggaatggg attcacagta atgaaaagct ttatggacga
     3241 tgtagaggta agttctgtaa aagataatgg gactagaatt gaaatgacta aaaaaataaa
     3301 tgtgcctaaa taaagtccaa aagtgaggtt ttattatgga agtaactgtt gccagagaag
     3361 aaaaaaaacc tcttcttagt catgaagaga ctctagaact tattgaaaaa gttcaaaatg
     3421 gagatgaaga ggcaaaagaa atattaatat caagcaattt aggtctagtt aggagcgttg
     3481 tatctagatt tttaaatata ggatatgata gagaagattt atttcagtta ggttctatag
     3541 gcttaataaa atcaatatat aaatttgacc caaaatttaa tgtaaaattc tctacttatg
     3601 cagtaccaat gattttaggt gaaataaaga gatacttaag agatgatggt atgataaaag
     3661 tatcaaggtc gttaaagcaa atagctgtaa aggcaaagat ggaaagtgaa gctttaacta
     3721 aaaaattagg tagagagcca agtatagaag aattagctaa agcaatagat gtagaaaaag
     3781 aagatttagt aatggctatg gaagctaatt tcaatgttga gtatttgcaa ggagttattc
     3841 atgaagagga aggttctcca atatgcctta ttgataaaat aagcatgaaa ggagaaagtg
     3901 aagaagaaaa agtagtggat aacatattgt taaaagacat acttggaaga ctggataaga
     3961 gagagcgtca aataatagtt ctcagatact ttgaagacat gactcaaagt gaaataggag
     4021 agatgctcaa tatatcacaa gttcaagtat ctaggataga gaagaaagta ttgtctaagt
     4081 taaaagaata tatatcgtga taatacttat gatatgtaga ataacaaaat ataataaata
     4141 tattactaaa ataaaatgta ataacttttt atgttaagtt tttaaattat taaaagatac
     4201 taaaataata aaaagcatta aaaaagtaaa aatataaaaa caaggtgtct ttatggaaaa
     4261 atagattttt tctttattag gcatcttttt tattttttga ccaaacttta gatagtcaaa
     4321 ataaataata ggtttatttt tttaaaatta acaaataata ctttagagag gtgtttagta
     4381 tggataaaaa ttataaaaaa tatgtagacc aaataagccc aaaacctaca tatttaaaaa
     4441 actatacact tgcatttatt gtaggtggaa ttatttgtat gatagggcaa gcaataaatg
     4501 atttgtatat gaaagttgga ggtcttgata aactaggtgc tagttctgca acatcaataa
     4561 cattaatatt tataggggca tttttaactg gattaggagt ttacgaccta ataggaaaaa
     4621 gagctggagc tggttctata attccaataa caggttttgc taactcaata gtatctccag
     4681 caatggagta taaaagagaa ggctatgtat taggtgtagg tgcaaactta tttaaaattg
     4741 caggaccagt tttagtttat ggtataggtt cttctatttt atgtggaatt atttattata
     4801 tttttaagat gttttagatg atttttattt aatttaaatt ttattaatta tgaagaaggt
     4861 ataaaatctg aaaatagcta ctaaagtaac taattgaaaa ataattgtgc agatttaatt
     4921 aatattaaaa atttgaatag taaaatgcaa aattaaaaag gtgtgaaaat atgaaaaata
     4981 aaagaattgg aaaaagaaca gtcaagttag aaaataaacc aaccataata tcaactggaa
     5041 ctatagtggg tcctaaggaa ggtgaaggac cactaaagga ctattttgat atgattatga
     5101 ctgacgattt atatggtgaa aagacttggg aattagctga aagtaaaatg gtagagactg
     5161 catcccaaca agctatccaa aaggcaggta aaaaattgtc agatgtaaat tatatgctag
     5221 gtggcgattt aataaatcaa atagtgccag catcatttgc agcaagagaa ctggctattc
     5281 catttttagg tatatatgga gcttgttcta caatggcaga aggtctttgt ataggttcta
     5341 tgcttataga tggtggtttt gctgatttag tactgtcagg tacatcaagt cactattgta
     5401 cagcagaaag acaatttaga ttcccacttg aacttggaaa tcaaaaacca atgacagctc
     5461 agtggacagt tacaggagca ggaagtgtat tattagctcc taatggagac ggtccaaaag
     5521 taaagtatgt tactgttggt aaagtaatag atgagggtat tgatgatggt aataatatgg
     5581 gaccagctat ggctccagca gcaatagata ctatctactc ttactttgaa gatactaatg
     5641 atgacccaaa tagctttgat ataattgcta ctggagattt aggtactctt ggaaaacaga
     5701 tagcagaaga ctttctaaaa gaaaaaggtg tagatatatc aaaagtttat actgactgtg
     5761 gtatagagat gtttaacctt aaagaacaag atgtccactg tggtggaagt ggatgtgggt
     5821 gttcagctac agtgtttgca ggatatatat atgataagtt gagaaaaaaa gaatttaaca
     5881 aagtaatgct tgtatcaaca ggagcactac tttcaccgac aagtacacta caaaagcaga
     5941 caataccatg tgttgcacat gcagtagtaa tagtaaatga gtaggaagtg atataatatg
     6001 ttaatggact atgtaagagt atttattgta ggtggaatta tatgtttaat agcccaagta
     6061 atgatggatt atttcaaaat gcaaactcca tatgttatgg taacgtatgt aacaactggt
     6121 gtagttttaa cttttctagg actttatgag ccattagtta agtttgcagc agcaggtgca
     6181 acagttccta taataggatt tggatattca cttgctacag gagtaataaa atcaattcaa
     6241 agtgatggtt ttttaggtat ttttttagga ggtacaactg ctacagctgg tggaatagca
     6301 gcagctatat tctttggata tattatgtca gtagtattta ctccaaaagc aaaaccataa
     6361 atctttatta aaagaataat aagtttttga aataataact ttttataaag caggtggata
     6421 tcttttagat tccacctatt ttttatataa agaagcgttt acataattga agttgacaat
     6481 tatttacaac atagtgtagt attatattat caaaataaaa tgaagtgaag taggggataa
     6541 agatggtaac tattaaagat attgcaagga atctaggtat cagttattca actgtttcta
     6601 ggtgcttaaa caataatcct aatgtatctg aaaaaacaaa aaagaaagta gtagaagagg
     6661 caaatagact agggttccat tttaatgtaa atgcaagaaa tttagcaaaa aaagaaacta
     6721 acaggatagg ggtaatcttt tcaaataatt ttaatcatca agatactaga aagtttttta
     6781 gtgatataat ggatagttct ataaattcta ttgagactaa taaatacgat tttataatac
     6841 agcctaataa taacattagt ggtgatagta atgtatataa aatggtaaat ggacaaatgg
     6901 ttgatggatt agtaattgtg tctcaatcaa taaaaaaaga agagtatgat tttttaaaag
     6961 acaataattt tccacatgta tttatatatt ttaaaccatc ttttgtagga gaagtagaca
     7021 atttcttttg ggacgataac gtgtatggag gttatatagc taccaagcat ttaattgacc
     7081 atggtcataa agacataata acagtaactt cagatgatag gagttcaaaa atgcatgaag
     7141 atagaactaa gggatattta aatgctatgg aagaggcaaa tttaaagacc gaagtcatta
     7201 aatctaaaat ggattttgaa tctcaagttg aatttttaga aaaatatatt gataaaataa
     7261 aaaaagcttc agctatattt gtacaacaag atgtacctgc gatttctata atacaagagt
     7321 taaaaactac ttatggtata aatgtaccag aagatatttc cataataggg tataataata
     7381 ttgaattaat atcatatttt aggtcgcatt tatcaacaat agatgaccca agagagcaag
     7441 ttataaaaaa tggagtagat agtttagtaa atataataaa taaaaagagt acagaacaca
     7501 ttccaagaaa attatatcca aggttaataa tacgaaatag cgttaaaaga ataaagtagt
     7561 aagcttttaa gtatcaaaag tttattaaaa atattataga ttatgtgaag ttttttaaca
     7621 aacaatataa ataaaaatta aaaggagtag gtgtatataa gtacattgta ctcctttatt
     7681 tattgagata gtttttataa tatgaatgaa taattttatt caattatttg aaaatccgtt
     7741 attttaacaa tataaattaa taaattattt gtattttata tcatgaatct ataatatttt
     7801 tttgattcat aactaaaagt atataaaaat gatgaataaa tatatattat acattatcaa
     7861 tgataggtta tataataaaa tcactaacaa ttatattata agaaaattat aaaaattaca
     7921 aggaggtata gaaatgaaag aaaaagcagg gattagaaat gccataatag cagcctttat
     7981 aatcacaata ttggcagaat taattggacc tatttcattt aaagttatgg gaataaatgt
     8041 aactttactt agtatactat gggctatatt cataggaatg gctgtttctc ctcatttatt
     8101 agggagagtt ataccagcct taaaaaagtt tataggggat aatgaaataa atgtatctcc
     8161 atatttatta tctttgacac tatatccatt aggaataatg tttggaatta atgcagggcc
     8221 taaagttggt atattacttc aagcaggacc agcactttta ttacaagaat ttggtcatat
     8281 gggtacaatg ttgatagcat tacctgttgg agtagcatta ggtcttggaa gaagtgcatt
     8341 aggtggtact ttttcattat gtcgtgacac agcactaggc attattggag ataagtatgg
     8401 attaaactca ccagaaggta taggaacact aggaacttac ataagtggta gtatatttgg
     8461 aacattatta ttttcatttt tagcaccagt gggtgtactt ttaggatttc atccatatgc
     8521 tttagcaatg gcatcaggaa tgggtagtgg ttcaatgatg ggagctgcaa cagcatcatt
     8581 aatgaatacg gttccacaaa tgtcagagca gattttagca tattcagcta ctagtggatt
     8641 gattacagct gttactggaa tatatataga attattctta tctttaccag tagccaacta
     8701 ctattatagc aaagtagctc cagttattga aagatttaga ggaagaaaaa caagaagaac
     8761 aagtaataaa ttgtaaagga ggatatatat atggaaaaaa cttttaagac tattatggag
     8821 cagataaaaa tagtattgtt tatatatatt attacttgct ttggacaaat gatagcactt
     8881 aagactcagg ctcaagattt gttgattgga agtatatctg gatttttgtt ggtgataatt
     8941 gctattataa taaagaattt tgttaaaaaa cctaatttac caggttttgc atgggctaca
     9001 ttagttgcat tcgttttatc gcttccaata tctccagtgg gggatatcat agcaacaaat
     9061 gtagggaaaa tcaattttat gtctacagta acaccattat tagcttttgc aggaatctct
     9121 gtaggtaacc aattagatat attaaagact ttgagttgga agttagtagt tatatcattt
     9181 atagttatga catctactta ctttggttct gcttttatat ctcaaacagt tttaagatta
     9241 aatggaatgc tttaaggagg tagtaaatat gaatgaatta aaagcaaaaa ttaaagagga
     9301 aataaagtta ttatctgaag agaaaaagac atcatttgaa aaagtttctg actatttatt
     9361 ttcaaatcca gaattagcat ttgaggagta taagtcacaa aaagcattat gtgatttgtt
     9421 agaagaaaat gaatttaatg taactaaagg tgtaggagga ttagaaacat cttttgaagc
     9481 tgtgtattca aatggaacta atgggaaaac agttgctttt ctagctgaat atgatgcact
     9541 tccaggaatg ggacatgcat gtgggcacaa tatcataggg acaagttctg ttggtgcagg
     9601 tatcatatta aaagaaatca tgaaaaaaca taatatagaa gggactgtaa aggtttttgg
     9661 aacacctgca gaagaaagag taggtggaaa aataactatg attaaagaag gtgtatttaa
     9721 taatgtagat gctgcattaa tcttacatcc aagtgatgct tcaatgcctg atgatatatc
     9781 atttgctcaa gttaatttaa aatttgattt tactggtaag gcatcacatg ctgctgcttt
     9841 tccttgggaa gggaaaagtg ccttaagtgg agtaatagca ctatttaaca gtgtaaatag
     9901 catgagatta catcttaaag attatgcaag agtacatgga attataactg atggaggtag
     9961 catacataat ataattcctg aaaaatctac agctatattt aatgtaagag ccttaagcat
    10021 agaatattta aatgagattt gtgaaatgct taaaaattgt gctaaaggag ctgcaatttc
    10081 tactggaact aatgtagaaa tagttcaatt agatgaaata tacaaagaga ttaagaatga
    10141 ttctgaatta gtgaatatag ttagacaaaa ttttgaagtt ttgggtgaag actatgttga
    10201 aagagattta agtcaaggta taggttcaac tgatacagga aatttaactc atgagatacc
    10261 tgcaatacaa gcatatataa aattaaaaga aaatactgca actcatacag atgaatttgc
    10321 tgttgcagct ggtggtgaag aaggcaaagt agcacttata aaagcaatta aggtgctagc
    10381 aatgtgtgga gtagatattt tgtatagtaa ataatatata attttaaaat atattaagaa
    10441 aggaaatcgg attagacatg atattaaaac aagtaataga ggtttatgac attttagata
    10501 aagcaaatgc taatggggaa gaagtaaaaa cttacttaca aggttatggt gaagtagatg
    10561 taactgtaaa agaattgtct tcaagtaaag gttcaacaga tttagtaaag cttacaattc
    10621 ctgggaaaaa tggaaaatta aaaggtggag atgctcctac tcttggtatt ttaggtagat
    10681 taggagggat tggagcaaga ccagaagtta taggttttgt ctcagatggt gatggagcat
    10741 tggtagcaat tgcagtggct gctaaacttt tggatatgca acgaaaaggg gacatattag
    10801 atggtgatgt tgtaatttca actcatattt gcccagatgc tccaactaaa gaacactatc
    10861 ctactccttt tatggattct ccaattgata tgatgactat gaatgaaaat gaagttgata
    10921 gtagttgtga tgcaatactt tctatagata ctactaaagg aaatagagta atcaatacta
    10981 gaggatttgc aatatcgcca actgttaaag aaggctacat attaaaaact agtgataacc
    11041 ttttagatat tatgcagact gtaacaggga aatctccatt tgtattccct ttgtctattc
    11101 aagatataac accatatgga aacaatctgt atcacttaaa tagtatttta caaccagcag
    11161 tagctacaga tgcaccaata gtaggagtag caattacaac agagatgcct gttgctggat
    11221 gtgctacagg tgcaactaat tttagtgatt tagattcagc tgggagattt gcaattgaag
    11281 ttgccaagct atttggtaga aataagtgtg atttctatga taaagaagag tgggaaatgc
    11341 ttattaagag atatggcaaa ttaaatagat ttcaaacatt tggtatacag taattagaaa
    11401 aatattaagt ttatataatt tgtgatacaa ctatttaaga agatataaac aattaggaat
    11461 aaaagtaaga ataaagcact tagttaaaaa ctttaaaaaa aaatatttat ttgtttatat
    11521 aaaaaggagg attctggtta ttgggattct cttttttatg aatacaaata atatattgta
    11581 attataaaaa aaggagcgtt tcaaagtgat ttttttaatc accttgagac aactccttat
    11641 attttttatt taaattctat tttttattga ctgtatagct attttcacca tcataaggtg
    11701 gcatgacctt gaacagcgtg gttttgtaaa gttgggcacc acaaaacaaa aaatcaacaa
    11761 gcccttgtgt ggtctgctga cgtgtttact atactatact ttcccaaaaa ccgcaagcac
    11821 tatgggaggc tataaggcgg cgaccattca ggggcgatcc cctcgaaacc gggatagggt
    11881 tcccatgtgg gacataataa agagcgcccc ggaggtatca actaccatcc ggggcgctct
    11941 ttattatgtc ccgtgggaac catgcccacg aagcagggcg tacaacggac agataggtcc
    12001 taccctcctg acagactgga atctcaatcc catcgaaaac aatcataaca atcatggcaa
    12061 gattatatac actcctgtaa aatatttttg tgtgtaccat gaagtactgc ttgccactat
    12121 tgagaacgga taaaagggaa gtacgtatta taccaataag gtgaacgaaa caaaatactc
    12181 atttgataaa gttaaataaa cctgtcggaa ccccaccaat ccggcaagag ctcactcaat
    12241 ttaacagttt tttctgtttc gacattaact aaaatctcaa tacaagcact atccttatca
    12301 agttgcatta aatattcacg acaagcacca catggtgagc caacactccc attgctcatc
    12361 acacaaacta atttagtaat tttactttcg ccatttgtta tcatattggc gatagcattt
    12421 ctttctgcac acattcccaa tgtacaggct gtatcaatac acacgccagt ataaacattt
    12481 ccgctgtcgg taaggattgc tgcggcaacc ccaccagcat cgataaaagg tgaaattgtg
    12541 cgtggattta attttcccct agcattatta taaagttcct tccatttcat caggttgatc
    12601 ctcctgttgt atccatataa ataatctttt atacgccttt tccgcctaag gcacacaaca
    12661 cattactcgc ttttttcatt ctaatattcg tgcgtgaaaa tgtcaaacgg tttacttcgt
    12721 ttcattactt catactgctg ttcataaccc tgtttgttca cttgggcacc attcgagcac
    12781 caatttaccc gtttcagggt tgtagcgaac acagcagaaa tgctcatttc atgggctttt
    12841 ctggctagaa aaaaaatttt atcgctgcca gatatttact attttttatt aagttgctgg
    12901 agttccacca ccatcaggag gagtagtaga gccaccacca ttatttcc
//
LOCUS       DAGQWR010000116        12885 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.116, whole genome shotgun sequence.
ACCESSION   DAGQWR010000116 DAGQWR010000000
VERSION     DAGQWR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12885
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.116"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(177..932)
                     /locus_tag="MIW95_002904"
     CDS             complement(177..932)
                     /locus_tag="MIW95_002904"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBY3152216.1"
                     /translation="MLKVIINGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1373..2362
                     /locus_tag="MIW95_002905"
     CDS             1373..2362
                     /locus_tag="MIW95_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBY3152217.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMIVLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVINATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2633..3349)
                     /gene="dapD"
                     /locus_tag="MIW95_002906"
     CDS             complement(2633..3349)
                     /gene="dapD"
                     /locus_tag="MIW95_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBY3152218.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLNEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3573..4322)
                     /locus_tag="MIW95_002907"
     CDS             complement(3573..4322)
                     /locus_tag="MIW95_002907"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBY3152219.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDNEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4389..5276)
                     /locus_tag="MIW95_002908"
     CDS             complement(4389..5276)
                     /locus_tag="MIW95_002908"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBY3152220.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5393..6394)
                     /locus_tag="MIW95_002909"
     CDS             complement(5393..6394)
                     /locus_tag="MIW95_002909"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBY3152221.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(6819..7700)
                     /locus_tag="MIW95_002910"
     CDS             complement(6819..7700)
                     /locus_tag="MIW95_002910"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBY3152222.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            8023..9216
                     /locus_tag="MIW95_002911"
     CDS             8023..9216
                     /locus_tag="MIW95_002911"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBY3152223.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            9422..10540
                     /locus_tag="MIW95_002912"
     CDS             9422..10540
                     /locus_tag="MIW95_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBY3152224.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            10671..10916
                     /locus_tag="MIW95_002913"
     CDS             10671..10916
                     /locus_tag="MIW95_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBY3152225.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            10903..11649
                     /locus_tag="MIW95_002914"
     CDS             10903..11649
                     /locus_tag="MIW95_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBY3152226.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            11934..12809
                     /gene="hslO"
                     /locus_tag="MIW95_002915"
     CDS             11934..12809
                     /gene="hslO"
                     /locus_tag="MIW95_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBY3152227.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPDDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
BASE COUNT         4702 a         1774 c         2023 g         4386 t
ORIGIN      
        1 taataaacaa tataattcca tataccaata aataagataa tctttaagaa aaatgattat
       61 atttaaaagg taagcataaa atttataaac ttttagaata gaaatctcaa tcaattagat
      121 attcaaattg atgaatatga atacctaaat agattgagat ttattatgta caaaacttat
      181 cctagtatat ctgacatttt ataaagtcca gattgcttac caattaaaaa tttagctgca
      241 actatagaac cgtttgcaaa tacttcatca gattgagaat gatggcttaa tgtaagaact
      301 tcattatcac ctgcaaatat gacatcatgg tcgctgatta tattgccacc tcttatagag
      361 tgtattccaa tttcatttgg ttttctttta caatctctac catatctacc atgtgtattt
      421 tcactttcag gcaagacttc tctaattgca ttagctatca tagtagcagt tccactaggg
      481 gcgtcttctt ttctattgtg atgtttttct atgatttcaa tatcaaaacc ttctaacatc
      541 tttgaagcat ccttaactaa ttttattaat atattcactc caagagatgt atttgatgat
      601 aataatatag gaatgttttt tgatgcttct tctattttat ttaagtcatt ttcatcatat
      661 ccagtagtag ctaatactac aggtgtatta ttttttagag catatgacaa tattggctct
      721 aaagtctcat gatgtgaaaa gtctattatg acatcagttt tttcagttac atcagtcatt
      781 gttttgtaaa gctttgtatc actatgatga cctttttcat cagcagacct agctacacca
      841 caaaccaact ctaattcatt atcttttttt acacattctg ctaaaactct tcccattgtt
      901 ccattatagc cattaattat aactttcaat ataaattcct cctatgaaat taataaaaat
      961 ttgttatatt tctataagtt tattttatca ctaatattat ttatataaaa taatattagt
     1021 gataataagc ttacaattta tcttacatta aaaaaattta agtggatttt tcaaaatata
     1081 gataaacttc tttttcattg tataaatgat aaaaatatat ttgacttcat atcaaaaaga
     1141 gtgccccatg aactaaaaag ttaattttga gataccatat tcatatatca tatgatttat
     1201 ttggaaatca atattaatac attaaaataa ctaaatacat gtatgcataa agatgtataa
     1261 aaaatatata aacatatagt attaatttta aattcaacaa tttaattgtt aaaagtgtta
     1321 ttattaaatt agaaatatct taacttaata tactgagatg ggagtgatag atatgttaaa
     1381 agaagaaaaa acttatcaag agaaagacgc aaaagtattc aatggagtgg cagctttgat
     1441 aatgattgta ttatttttag tagtttcaat aggtatcatg gtttttggga tagtaaaact
     1501 tgatagtgga tttactggaa taggttcact tatgttaatt ttgggacttg tatttatagt
     1561 agtagatttt atcctatttt ttgggctaaa aatgattaat cctaaagaag ctatagttct
     1621 agttttgttt ggtaactatt atggaactat aaaaaaagaa gggtactatt gggtgaatcc
     1681 attttgttca gcaataaacc cagcagtatc aagaattaat gtatcgaaat cttcatctga
     1741 tgaaaaggta gatatatcaa ctaattcaag gggcaaaaaa gtatctttaa aaacaatgac
     1801 tcttaataat gaaaagcaga aagtcaatga tttattggga aatccaatta taataggagt
     1861 ggttgtaatt tggaaggtta taaatgcaac aaaggcagta tttaatgtgg ataattataa
     1921 tacattctta tctattcagt gtgattctac tattcgtaat gtttcaagac tttatccata
     1981 tgatgtatct gaagatggag atgaaaaatc tcttcgtggt agtagtcagg aaatagcaga
     2041 tagattaaaa gatgaattac aatcaagggt tgacattgca ggaattgaag tgtgtgaagt
     2101 gagaattact catctttctt atgcaccaga gattgcagca gcaatgttac aacgtcaaca
     2161 agcagaggct ataatagcag cacgtaaaaa gattgttgag ggggctgtaa gtatggtaga
     2221 gatggcattg actaatttaa gtgaaaataa tgttgtgact ttggacgaag aacgtaaagc
     2281 agctatggta agtaatctgt tagttgtgct ttgtggcaat aaagatgcac aaccagtagt
     2341 aaacagcagt tctatttatt aaattataaa aagtttaata gtttattaca ttgatatatt
     2401 gaaaaattga aggaaataaa aatacttgtc atcaagaaat aaaaatgata taagaatgaa
     2461 taagagaatt gtaaaaaaat aaaatgatta taaataaaag ataaaaatat tataaaaaag
     2521 agggtgcctc tgatgaatta aattcataca gagacacctt tttaattaat tttttaaatt
     2581 agtatattat tgtttaatta atctttctaa ttaataatat agtactattt aattagtcta
     2641 aatttctaag gtcttccata agcttagttt tgtcagccgt tttttcatct ttcattttta
     2701 taactttagc aggagaacca gcaacaacgg caccagcttc tacatcagta gttacaacag
     2761 caccagctgc aacaacagca cctttaccga ttctaacacc ttcaagaata actgcattag
     2821 ctcctattaa aacatcatct tcaactataa caggagtagc agaaggtggc tctaaaacac
     2881 cagcaacaac agcaccagca cctaagtgaa catttttacc aagagttcct ctagctccaa
     2941 taacagcatt catatctacc atagaacctt caccaataac agctcctata tttataacag
     3001 ctcccatcat tacaacagca tttttttcta tagtaaccat gtctcttatt attgcacctg
     3061 gctcaattct tgcatgttca tttaaagtat tttttagagg aatagcagaa tttcttctat
     3121 catattctac atgagtatct gttatatcat tcccatgttt atctaatact tccattatat
     3181 cctcatagtc acctacaaat atataagaac cattacttcc aaatacctta aatttatcag
     3241 ttgattggaa gtgtaaagag ttagtattta catatacctt aacaggtgtt ttctttttaa
     3301 cttcttttat ataccttgct atttcataag catcgtttaa atttaccatt tgttaattct
     3361 ccttgtattt cattatattt cttttattat atacttattt gtcaatttta gtatatactt
     3421 acttcttatt atttatattt tattttagtt agtttttatt gtatagacat agtattaaat
     3481 aaatttatta tatatgtttg atttgtattg cgtattatta tatctatatt gcatatgttt
     3541 aatttatatt gtgtattatt atatttatac tattatccca acatgctgtc catattataa
     3601 aatcctggtt tttgcttaac taagtatttg gcagcagcta tagaaccact tgcaaagata
     3661 ccacgagatt gagcttgatg gcttattgta agaacctcat tgtctccagc aaatagagca
     3721 tcatgttcgc caacaatatt tccacctctt atagcatgaa taccaacttc atttggttgt
     3781 ctttttgcac tacgaccata tctaccataa ttattttcaa cgtttggcaa aacctctttt
     3841 atagcgttag caatcataac agctgtgcca cttggggcat ctacttttct gttgtgatgt
     3901 ttttctatta tttctatatc aaatccttct agtaatttag ctgcatcttt aaccaatttt
     3961 actaatacat ttacaccaag agacatatta tatgattgga atacaggaat aactttagcc
     4021 gcttcacgta ttttgttcat ttcgttatca ttatatccag tagtagcaat tactaaaggt
     4081 gttttagtct ttaaaacgta agataagata ttatctaaat tagagtgatg agaaaaatct
     4141 ataactacat cagcttcctc aactatagta ctcatatctt cataaatttt aaggtcgcct
     4201 tcatagctat ccagacctct agctgcaaca caaaccaatt ctaattcatt atcttctctt
     4261 atagtatcgg ctaaaatctt acccattttt ccatcataac cagttgctat aacttttaac
     4321 ataatttcct cctatgatta agggctaact tttgaccaaa cttacttttt accatgcatt
     4381 aatctttact atgcattaac ttttagtcca aagttaacta attctttctt taacacttct
     4441 aaatttgctg ggtccatttc tgctagaggt aatcttaaat ctccaacatt gaatcccaat
     4501 aaattcattg cagtcttaac tggtatagga tttacttcta taaataaagc tgcgattaga
     4561 gcatccatac caagttgtaa ttttctagaa ccttcaatat cgccttcaaa gaatttagct
     4621 accaaatcgt gagtttcttg agggcaaaca ttagcaagta ctgatataac tccttgacct
     4681 cctaaagata ataaaggaag tatagtatca tcattaccag agtagatagc aaagtcatct
     4741 ggaactaaac tagctatttc tgcaacttgt cctaggtctc cactagcttc ttttacagca
     4801 actatgttat caattttagc aagttcaact ataaggctag gacttatatt tacacaagtt
     4861 cttccaggga cattgtaaag tataattgga agtttaacac tgttagctat tgtttcaaaa
     4921 tgacgcttaa gtccagcttt attagcttta ttgtaataag gtgttattat taatagagca
     4981 tctactccta atttttcagc ttcttgactt aagttaactg agtgcatagt atcattagaa
     5041 ccacttccag ctataacagg aactctttta ttaacttttt caacaacata cttaatagta
     5101 gcaagttgtt cttcatcagt catagtatta gcttcaccag tagtaccaca agatactata
     5161 gcatcagtac cattagctat ttggtattca actaattcac ctaatttgtc aaaatcaact
     5221 ttgttatcct ttgtaaatgg tgtaactaga gcaacagcag aacctttaaa tatcatataa
     5281 aatcaatccc ctctttttta tttttgtgtt ttatattaca gttacttcta atgcttgttt
     5341 ttgagttact attaatattt tacaaaatta tatatttata caagtttgta tattatacta
     5401 aatcgtatga taaagccatt tcagctattt gaattgcgtt agttgcagca ccttttctta
     5461 tattgtcagc aacaacccaa agatttacac cattatcaac actaaaatct cttcttattc
     5521 ttccaacaaa tacttcatct ctatcatcaa attctacagc agttggatat tcgtttttag
     5581 caggattatc aactactaca acacctggag cttcttcaag tgtttttact aaatcatcta
     5641 actcaaatgg attgttaaac tcaacattta tagattcact atgaccattt tttactggaa
     5701 ctcttacagt agttgcagtt atttttaaat catagttatc aagtattttc atagtttcat
     5761 ttaccatttt catttcttct ttagtgtatc cattttcagt gaatgaatct atatgaggaa
     5821 gacagttgta agagatttga tgagggtaga atccagtagg attaccgttc attcctttct
     5881 ctaaatcttc aacaccttta acaccagaac cagatacagc ttggtaagta gagtaaacaa
     5941 ctctcttaat tttgtattta tcatcaagag ctttaagggg aaccatagct tgtatagtag
     6001 agcagttagg attagctatt atacccttat gattttttat ggcttctgga ttaacctcag
     6061 gaactactaa aggcacatct ggattcattc tccattgact agagttatct actactaaga
     6121 ttccatttga agcagctatt ggagcatact tttcactaat acttccacca gcagagaata
     6181 atgctatttg tatgcctcta tcagtaaatg aagtttcctt taattcttct actatatatt
     6241 ctttaccaca aaacattact ttagaaccag ctgatttaga agaagagaat aaaaatagat
     6301 tttctattgg aaaatttctt tcttctaata cttttaaaaa tgttctacct accattccag
     6361 tagcaccaac tattgctaca tttacttttt tcatataaat tcaccccaat tttaaattat
     6421 taaaacatta ttttatttta gttatacaag taagatatgt taatattttt atatatccaa
     6481 aaactttaat attatattaa caactttaat tatatacaaa aaacgtacaa aggtatactt
     6541 tgtacgtcta atttatcact caaagtaaac cctgtagctc atcacctaca aaaataagtg
     6601 acagtattac acatgttatg tgcaatccca gaaaatatta ttgcaagaaa tattttcttc
     6661 ggctgtaaat cctttttcca atcattaaca cctgccagct actattggat actcttagtt
     6721 acagctacct ctaccctagg ttaataggat attaaaattt gattattcta ataataatac
     6781 atatttaaga aaaaatcaac ttatttatat aaatattgct aaaacttaaa tccaaaattt
     6841 gtcaattctt gttttaacac attaagatta gtttcttcca tttcagctag aggaagtctt
     6901 aaatcaccaa cattaaaacc taaaatgttc atggcagttt taacaggaac tggatttact
     6961 tctataaata aagcagcaat taaagcatcc atatcaagtt gtaatttttt tgagccttca
     7021 atatcacctt caaagaattt agttactaaa tcatgtgttt ctttaggaca tatgttagca
     7081 agtacagaga taacaccact tccacctaat gataatagtg gtagtatagt atcatcatta
     7141 ccggagtata tagcaaagtc tttaggtact aatttagcta tttctgcaac ttgagctaag
     7201 tctccacttg cttccttcac agcaactata ttatctattt tagcaagttc ggccacaaca
     7261 gatggcttta tattcacttt agttcttcct ggtacgttgt ataaaataat tggcaactta
     7321 acgctatttg ctattgtttc aaagtgaagt ttaagacctt tctcattagt cttattatag
     7381 taaggagtta ttattaaaag accatccacc cctaattttt cagcttcttg actcatatga
     7441 acagacaaca tagtattgtt tgaaccagta cctgctataa caggtattct cttattaact
     7501 ttttcaactg tatattttat tacagcaaaa atttcagatt cactcatagt agtcgcctca
     7561 cctgttgttc cacaaacaac tagtgcatct gtgccattct caatgtgata ttcgattaat
     7621 tcacctaatt tttcaaagtt gacattatta tcctctgtaa atggggtaac taaagcaaca
     7681 gcagaacctt taaataacat ataatcaaat ctcctttttt atctaaattt aattttttaa
     7741 gtacaataat aattatagta caaattaatt aattttaata ggtatagtta atatcttaac
     7801 tgtttaaata agatatttat attatattaa tattagaaaa attttaaata aatgttttat
     7861 tctcaaaata tgcatattaa tttaataaaa tataccaaaa tggaaataaa aaataaattt
     7921 tatgagaatt taataactaa taattaatat atttaggtta taataaatat gtgcgtaaaa
     7981 tctaacaata agcaataata ccgtaaattt aggaggaata atatggatac tttaaaggaa
     8041 ctttttaaaa taggaagtgg accttcaagt tctcatacta tgggaccaca aagagcagca
     8101 gagagattta agaatgaaaa cccagatgca gaaagtttta gagcaatatt atatggtagt
     8161 ttggcagcta cagggaaagg tcacttaact gactatataa ttgaaaaaac tatagcacct
     8221 aaaaaagtcg aaattgtttg ggaagaagat attataaaag attttcaccc aaatgggatg
     8281 aagtttgaag ctttagataa agataaaaat gtaacagctg aatggactgt atattctgta
     8341 ggtggaggga ctatagcaga agaaggtcaa agaaacagta aaagcaatag tatatatcac
     8401 ttagatacta tggatgagat agtaaaatgg tgcaaagaaa ataataagac tttggtagat
     8461 tttgtgttgg aatgtgaacc aaaagacata aaagattata taaaaactat aaaagatgct
     8521 atgagaaaat ctattgatga tggactaagt acagatgaaa taataccagg caagttatta
     8581 ttaaaaagaa gagcaagtac attttataat gcatataaaa aagataagag cttctcaaca
     8641 cttgtatatg catatgcact agcagcttca gagcaaaacg cttctggtaa tataatagtt
     8701 acagcaccaa cttgtggttc tgctggagta ataccaggaa tattctttgc aatgcaggat
     8761 ttctataatt atgatgacga aaaaataatt gaagcactac ttgtagctgg aataataggt
     8821 aatataataa aaactaatgc ttcaatatct ggagcagagg tagggtgtca aggtgaagtt
     8881 ggagcagctt gttctatggc agcagcagca gtagcatact taaaaggtgg aactatagac
     8941 catattgaat atgcggcaga aatagcactt gaacatcatt taggaatgac ttgtgaccct
     9001 gtatatggat atgttcaaat tccatgtata gaaagaaatg caatggcagc tcaaagagct
     9061 tatgatgcag ctaattatgc attattaact gatggctcac attctgtctc cttagaccaa
     9121 gttgttgaaa caatgaaaga aactggtata gatatgatgg ataagtacaa agaaacagct
     9181 aagggtggac ttgctaaaca tttcttctct tgctaaaaat aaaattttcc aataataatt
     9241 cctctgtttt gatttatgaa acaggggaat tattattaat ggtgaattat ttaataatgc
     9301 aaaatatttt tatattcaat ttagcttttt atttttaatt agtataacat gctatataat
     9361 attttaatta taaagaaata tagaaatatt ttttaataaa ttagaaattg gaggagatta
     9421 catgagagag gcatcatatt tacaagaaca actttataaa cataggaagt cattaaatcc
     9481 tattgcagag attgggagaa aagagtataa gacttcaaaa tatataagag attatcttga
     9541 taaattagga attgagtatg aagtttattt agaaacaggt atagtaggtg taatagaagg
     9601 taaaagacct aaaaaagata ttgcttttag agctgacata gatggtcttc atagtgaaga
     9661 aggggtaaaa catttatgtg gtcatgatgg acatgctact atattacttg gattaattga
     9721 gtatttaaat gataataaag aagacttaaa tgataatata gtatttatat tccaaccagc
     9781 agaagaaggt cctgggggag caaaagcttt aatagatgaa ggagtgctta gaaaatacaa
     9841 tattgatgaa atatatgggc ttcatatata tccagagtta ccagaaggtt atgtaggtac
     9901 aagagaaggc tattttatgg ctcaaatagg agatatcgac atagaaatag tgtctaaaag
     9961 tggacatggg gctatgccac aaaatggtat tgatggtata gtgatagctt caaactttgt
    10021 aagctcactt caaactatag tgtctagaaa tataagccca atagacaatg cagtgttgac
    10081 tattggtaga attgaagggg gagctagaag aaatataata gctgaaaata taaagatgga
    10141 aggtactatg agatgtttca gcccagaagt gtatgagaaa atgaagctta gagttagaga
    10201 acttgctaga gggtttgaat tggcttataa ctgtaaggtt aatataaata ttatagatga
    10261 ttatattgct gttaaaaatg acaaggattt gtatcaagag tttgtagagg ctattggaag
    10321 tgatactgtt gtagagttag agccacttat gatatcagaa gatttttctt attatcaaaa
    10381 agaagttcca ggattatttt ttatgttggg ttcaagaaac gaagaaaaag gatttgtaaa
    10441 tggtcttcat aatataaact ttaattttga tgaaaagata tgtgtaaatg cattgaatgt
    10501 ttattcaaaa ttattaaaat acaaagaagc aatagattag gaagataaat tataaagatt
    10561 aaaaagcata aatacctaaa tttaaaaaaa atgtattaat aaatacccct tatatatttg
    10621 tataaattta ttttaattga caaaaataca aataagaagg ggtgattttt ttgaaagaaa
    10681 aagtacaaag agaaaagaag ataactaatg gcaagaatac taaaatattg acaaatgatg
    10741 atataatgaa atatgagata gcctcagaac taggtcttat ggataaagtt ggtgaacgtg
    10801 gatgggcagg acttacagct aaggaagcag gaagaattgg aggtatgctt acatcacgca
    10861 aaaagaggct taaaaagatt gtggaggagc agaataaaaa ggatgagtca atatagtgat
    10921 tttgcgtttg tatatgatga acttatgaat gaagttgatt ataatggttg ggttaaatat
    10981 atagaagata taatagatag tgaaggtgta aaagtaaaaa atatattgga attggcatgt
    11041 ggcacgggta atcttacaat tccattgact aaaaaaaatt acgatatagc tggtatagat
    11101 atatctgatg agatgcttag tgtagcaaga gaaaaggcag aaaaagaagg agtagaatta
    11161 gttttacttc aacaagatat atcagagctt gattttgaaa tatctgattt ggactgtgtg
    11221 ctttgtgctt gtgatggatt taattatata acttatgatg atgatttgga aaatgtattt
    11281 tcaaagacat atgaattatt aaagaaagat ggaatattca tatttgatat aagttctttt
    11341 tataaattag ccaacatatt gggaaataac atgtatggag aaaatagaga agatatagcg
    11401 tatatgtggc aaaattactt tgatgaagaa gagaatctaa ttgaaatgga acttacattc
    11461 tttattaaag atgaatgtgg aaagtttgaa agatttgaag agatacacca acaaagagca
    11521 tatactgaag atgagatagt tgatatgctt gaaaaatctg gatttaagga tataaaatat
    11581 tatggggatt ttacatttga caaacccagt gaagaaagtc aaagaatctt ctttgtatgt
    11641 aaaaaataag atatttttga ctatataaga taattattaa aaataaccat gatataaaaa
    11701 tatagaaaat gcagttttat agattaaaag taaagtgatg atactttatg acttttattt
    11761 tctaataatt ataaagttct atagataaaa attaaaatat aataactatt gcttagtaaa
    11821 attaaaataa aatgggtttt gcccaaggtt aatatagata agtacttata gattaaaatt
    11881 aaggtagtaa tataataaaa ataagacaat taacgggaca tggaggaata ttaatgagag
    11941 attatgtact aagagcgaca tcaggaaatg gtcaagttag agcatttgtc gcaactacta
    12001 gaaatacagt agaagaagct agaagattac atgaaactac aaaagtagca acagcagcac
    12061 ttggaagaac attaacagcc acatctataa tgggattaat gatgaaaaat gatagtgata
    12121 aattgactgt aataataaaa ggtggaggtc caattggaac tattatcgcc acatcagatt
    12181 ctaagggaat ggtaaaagga tatgtaggaa atccacaagt agaggttgaa gactatccaa
    12241 atggaaagtt aaatgtagca gcagcagtag gtacagaagg tgttgtaaaa gttataaaag
    12301 acttaggtct tagagaacca tataatggga cttatccatt ggtaagtgga gaaattgcag
    12361 aagactttac ttattatttt gctgtatcag agcagactcc atcagtagta gctttaggtg
    12421 ttcttacaaa agaagatgaa gtagagtttg ctggaggatt tatagttcaa ttaatgccag
    12481 atgcagaaga agagactatt gctaagttag aggaaaatgt agctaaatta ccttctataa
    12541 ctaacatgtt aaaagaagga aaatcaccag atgatatatt aaatattgtt ttggatggtt
    12601 tggaacctaa aatacttgat acctgtgaag tagggtttat gtgtgaatgt tcaaaagaaa
    12661 gagtaaaaac tgctttggtt gcaattggta aaaaatcatt ggctcagata atagaagaag
    12721 ataaaaaagc agaagttggt tgccagttct gtaataagaa gtatatgtat agtgaagaag
    12781 agttgcttga aattttaaag gaaatgtagg ctataaaaat agttgtattg atataagtaa
    12841 tgttatagtg atatagtaaa gtcgtattga tatagtaatt aaagt
//
LOCUS       DAGQWR010000117        12811 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.117, whole genome shotgun sequence.
ACCESSION   DAGQWR010000117 DAGQWR010000000
VERSION     DAGQWR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.117"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(204..668)
                     /locus_tag="MIW95_002916"
     CDS             complement(204..668)
                     /locus_tag="MIW95_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HBY3152228.1"
                     /translation="MKKLVIILISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            complement(992..1600)
                     /locus_tag="MIW95_002917"
     CDS             complement(992..1600)
                     /locus_tag="MIW95_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBY3152229.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            complement(1682..2383)
                     /locus_tag="MIW95_002918"
     CDS             complement(1682..2383)
                     /locus_tag="MIW95_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152230.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            2610..2876
                     /locus_tag="MIW95_002919"
     CDS             2610..2876
                     /locus_tag="MIW95_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBY3152231.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            complement(2979..3398)
                     /locus_tag="MIW95_002920"
     CDS             complement(2979..3398)
                     /locus_tag="MIW95_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBY3152232.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERIFNLKQEKRKAKHKGH"
     gene            complement(3665..4447)
                     /locus_tag="MIW95_002921"
                     /pseudo
     CDS             complement(3665..4447)
                     /locus_tag="MIW95_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(4512..5273)
                     /locus_tag="MIW95_002922"
     CDS             complement(4512..5273)
                     /locus_tag="MIW95_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBY3152233.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNKKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            complement(6322..7134)
                     /locus_tag="MIW95_002923"
     CDS             complement(6322..7134)
                     /locus_tag="MIW95_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY3152234.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPNLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNDEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            complement(7504..8406)
                     /locus_tag="MIW95_002924"
     CDS             complement(7504..8406)
                     /locus_tag="MIW95_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY3152235.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDGQISFFD"
     gene            complement(8456..8917)
                     /locus_tag="MIW95_002925"
     CDS             complement(8456..8917)
                     /locus_tag="MIW95_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBY3152236.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(8969..9424)
                     /gene="def"
                     /locus_tag="MIW95_002926"
     CDS             complement(8969..9424)
                     /gene="def"
                     /locus_tag="MIW95_002926"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBY3152237.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(9396..9659)
                     /locus_tag="MIW95_002927"
     CDS             complement(9396..9659)
                     /locus_tag="MIW95_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3152238.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(9756..10472)
                     /locus_tag="MIW95_002928"
     CDS             complement(9756..10472)
                     /locus_tag="MIW95_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152239.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGTLAFIITMILLIFFSLTKISFLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(10469..11146)
                     /locus_tag="MIW95_002929"
     CDS             complement(10469..11146)
                     /locus_tag="MIW95_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152240.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILISIIIIIYFITYYFICSSWK
                     KVGGVKL"
     gene            complement(11143..11988)
                     /locus_tag="MIW95_002930"
     CDS             complement(11143..11988)
                     /locus_tag="MIW95_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152241.1"
                     /translation="MIKVDDLSFSYTDRNFLQNINFEVEKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIKKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(12102..12692)
                     /locus_tag="MIW95_002931"
     CDS             complement(12102..12692)
                     /locus_tag="MIW95_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY3152242.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRVVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
BASE COUNT         4361 a         2109 c         1186 g         5155 t
ORIGIN      
        1 ttaattgaaa acttaaattt atttccttgt gttatcaaga tttcatgctt accatttcca
       61 atatcatgct caccaacttt tgatgtgcat aaactatttc aagtaaaaat gcacaaccta
      121 tcctttttga taggaaaaag cagtcaatca taaatatcaa ctgcttttat gtactgaata
      181 tcaagttata gacaagctgg aatttatttt gagggttttt ctttctcctt aactaaattg
      241 ctttcaaaag taacctttac aactgcatga tttattttaa ttccggtaaa gcatatagca
      301 tactctttct ctttttgtat attagaaaga gaaattgcga atgtatcatt atcaacgctt
      361 ccatgccatg tattactcca taaagtttca tctgttttat tgtcctttat ttccacaata
      421 ccactatctc catccatttt aaaagaaatc tctgtgtcta aaacatctat atcattagac
      481 acacaaaaca aacgtgcatt aacaaatggg tctgaagtat cataattttt atctaagtcc
      541 atttcaatgg ttgtttgtcc tgattctttg ttgtttaaat tattatattt actttcattg
      601 ctttttttac aagctgtcaa gctaaccaca ctaatgatac taattaaaat aataactaat
      661 tttttcatag aatttatctc ctttacaatt tctaattttt aaccctacaa actataatat
      721 taaactaatc tactatttta catcaaattt ttcaatcatt cagcctttac cttttctcat
      781 tttaattgca aatatgctaa ataattttgg aattttaatt attgtagtat cttaaattgg
      841 tttataacat attatgaaaa tttaagagtc aagtaactta tttaaaaata cacaaaatta
      901 tactttattg tataaaaaaa gagattataa tttatacaag taaaataaac tttattattt
      961 tactcttcta tataacctct tttctatata tctattcaaa taatttagct cctaaactca
     1021 caaacatttg taaaatcaaa aatgcttttg tgtctatctt atgattactt tgtaaaatct
     1081 cttttgcatc ttcttgcgaa attaaaagcg cttcaatctc ttcatctggt tctaaaaatt
     1141 catctgttat atcaccatca catatacaat aaacaaaggc aacagattca tcactcattc
     1201 caggagataa ataaacttta tcaatacttt tatttttttt aatttctaaa agagtaagtc
     1261 ctgtttcttc tctaagttct ctttcaacag aaatatctat agactctcct tcatcaacaa
     1321 gacctgctgg taactcgtat atatatccat taataggtac tctaaattgt cttattaaaa
     1381 ttaatttttt actactttta tgaagcccaa ctattacaac tgcatctaca ctatcttctt
     1441 cattcttcaa ataaatattt tttaattgct cactactttt tcttgatgct accatccaaa
     1501 ccttatcttc atctaatttg tttttatact ctatttcaaa aagtcctata aacttatttt
     1561 caatcattgg atttacattc tttattatag tttttttcaa atttactacg acctttcttt
     1621 tttaatacct ttttaacatc attaagtaaa tataatatct taacaagtat tcaacaactt
     1681 attatatttc catttcagga ttatctttat ctacaaaacc tactttgatt tcacaattaa
     1741 atatttcctt taacaatttt gctaattctt ctcttgcact cattacctta tctaaattag
     1801 catcagtaaa accatctata ggaaaaaata tattttttga gctattagta atttttcctt
     1861 cctctccttg tctccatatc catcttctag tttttaccat gtctccaact gaataaatta
     1921 attctccttc ttccattaat tcaacatcag tttctccaaa tggtacaaat ttatcccctt
     1981 ttttagaaaa tctaacatat acatcatcat ttctaaaatc catatcatga gcacccattg
     2041 gaagtaaata ttttaaagaa atagagtttc ctaaatctac aataggattt atatgaggaa
     2101 gtttcttatt cttagcaacc cttgttgtca ttgcttctat cgaactcata aatttatttg
     2161 ggttcatgcc tagacttcta aatgcctctc tatatggtat aatttcttct gattccttta
     2221 cttttttatc tttgaagtaa tcttctactc tatctatact tatatctaaa aggttgctta
     2281 ttctttctat ttctttagta ttatctattc cttttgcaac tactacacca aagcatacat
     2341 tttctaattt atcaaaaact tctttttcta ctataaattt cattttgctt tatcctcctg
     2401 ttattttaca ttattttcta aatagatttt atcactttgt gtcttataaa tataccccct
     2461 atttgctaat tgtttaattt ttttaaattt tccaattaat attatattta ttcttatcag
     2521 atattttata tgtactaatt acaaatttaa ctttccaaaa caaatatctt gttttataat
     2581 gtataatata tttttatgga ggcaagactg tgaaaaattt tattctaata tattttatag
     2641 tgattaattc tattgctttc ttttcaatgt acatagataa gaaaagagca attagaaacg
     2701 aatggagaat aaaagaagct actcttatgt ctatagcagt aattggaggt agtataggtt
     2761 ctatgattgg aatgtattct tttagacata aaactaaaca cattaaattt acatttggta
     2821 taccatttat actattttta caatttttat tagtatattt ttatatttta aagtaattga
     2881 aatataaaac cttattcttt tcaataaatt aaggtgtata aaattaaaaa ctttatacac
     2941 ctttttctag caattattaa aaagatattt aattaaaatt aatgaccctt atgtttagct
     3001 tttctctttt cttgtttcaa attaaatatt ctttcttttt cttcttcttt tctattttta
     3061 gctaggtttt ttctatcctg ttttctttct tcatattgaa gcttaagagc attttgagcc
     3121 ttagtcccta tagtttcttt ttcttgttcc ttttttactt ttctctgcat tcttttgtat
     3181 ccaatgttgt ctttggtcac atttttttct aatttaatgc taccaaaatc taatgagaaa
     3241 aatctttcaa gtataaattc atacacttct acttctttag gttctgctcc aaaaactacc
     3301 ctacatgctt catatttttt accatcttgt ctttcaaata tacctaccca aaatggttct
     3361 tcaaataaga ctgttaattt tccactaatt ttgtccatcg ctagttcctc cttaaattac
     3421 tttctaaaga acggacaacc aaggaggcag gttactgaca gctattgctg cgtctggact
     3481 accaaccaga ctgtgttttt atctttatag attaaatatt atctttttta atcatgcttg
     3541 tcaatcttag tatcaacatc tatcatattg atttaagtga aatcaagctt tttaactcta
     3601 ctttattatt gttaacacta agaaaattaa ttatttttca taataaatta gttattatac
     3661 ttatttaaat atcaatcttt attgttccct gcataaacaa gactgcttca ccagaaattt
     3721 ttacagtatt atccttcaac acttcacact gaattatacc accacgatta gaaagttgag
     3781 cagctaccat tttgtgtttt cccaactttt cagaccagta tggaataaaa ttacaatgtg
     3841 atgaaccagt aacagggtct tctgaatcta attctggaca aaaatatctt gatacaaaat
     3901 ctgtattact tccttgtggt gtgattatta tacctaacca gtctgtcaat ttacgcaatt
     3961 gtgcgtaatt aggtttataa tttataactt cttgttcact gtttagcaat aagattaaat
     4021 ctcttgaaga atagacagca cttggtacac agcctatcaa attagcttgt tatggtgata
     4081 actctatttc aataggcatt atttctggaa atatcatttc atatagattt ccatttcgtg
     4141 taacttcaag ctttccactt tgagtaaaga aatctatcct ttttacatta acatcaataa
     4201 aatttgatat tacataagct gctgctaaag ttgcatgacc acataaatca atttctgctt
     4261 taggagtaaa ccatcgtaat tcataatttc ctttaccttt aactacaaat gctgtttctg
     4321 gtaaattgtt ttcttctgct attttctgca ttaactctaa aggtattatt ctgtcaagaa
     4381 cacaaactcc agcaggattt cctttaaata gtttagttgc aaatgaatca actatgtagt
     4441 attccattat ttgacctctc tttccaacat aaaatataca tcaatttaca ttcaataaaa
     4501 ctttaaattt cttacatata ttttttaata ttgctctgct caacaacttt tttacctaac
     4561 aatgtaattg ctttttttgc acaattattt acacaacgat aacacattgt acattgccca
     4621 tttgcaacaa tcatacaatt tttaattact agattcttca ttggacataa attaacacat
     4681 ttaccacata caatacattc ctcagtatta atttttagtt tatctgtata catatttgtt
     4741 ttattgataa aatacaatcg ttgtccaaat aaccctacaa tatgactcaa aaaacctaac
     4801 ccttcttttg gtggtgaccc atgtttcagt tttttagcag ctttagttac tttcttttct
     4861 gcttttatta caagcttttt attatactct aaagaatgtt ttaatacctt ttcatctcct
     4921 atactatctg gcatcttcac atgtaatcca ccaacagtta tagcaccgta tttttttaat
     4981 attctagcta acacaccaga gccatctcca ctaaatagag ccatagttgc aataataaaa
     5041 acacgtttac cattccacaa agttgaatga tttattataa aatctttcaa tatttttggc
     5101 acattactat attgcacagg ataactaaaa ataatattct catgattatt gatgtgctgt
     5161 aaaagttcat tatcttcaat agaataagaa tttgctgtta tatcatattc ttgtaaaaat
     5221 ttttctacac agtatcttga atttcctgtt ccactaaaat atattcctat catagtactt
     5281 ctcctaatag ctaagattat atagctctat tcttaaaatt ctgacttttt atattatcta
     5341 aattaagtag tttatttaaa taatataaac ttaattcaac aagttaccta ctgaacctcc
     5401 catgactaaa gtcatagggt tcctgcttga tagaatttta cataatcaat ttactaaata
     5461 cttgtctaat tcttttttta tcattttaaa tatacatttt aataatatta gtaagctata
     5521 cttccttaag catatatttt ccctaaagta aaactattga tgattattcc attatataac
     5581 ctaattataa atttagtaag ttcatcacat actataccct taattctcaa ttataaagtt
     5641 ccctaaaaaa tatttgaaaa tttaactatg aaaagatttc ctattgccta atatgtataa
     5701 gtaaggagta ctatatagta ctccttatat tgtactctta taacattgat accttaatat
     5761 tattttaatc ttaatcatta ttgatttgat ttaaaattat aaataggttc caatatttta
     5821 attatgtcaa ctatatctct tatagtttta ataatctcat caattggttt atgtgcttgt
     5881 tgcactttat caatagtatt aattaaatcc atttacacat ctagcacctg tatggcaatt
     5941 tagcattatc cttatcttat actttgttgt aaattcttga ttacataatt ctattatcta
     6001 acttatagct gtgcaatcta cattatctgt aaatatcttt gctttatata ttttccctga
     6061 acctctaaca ttaatataac agcctttttg ctatacactt ttaactatat acttaatttt
     6121 aataacatta cttgtttata aaaatatact atagataaat ttcacataaa aataataatt
     6181 caagtattga tttttatata agaaaatttg taatgtacta aaaataacat aaaagtttcc
     6241 atttttcaat atgtaagctt ttcttatgtt attttattgt taaaggtttc aagtttttat
     6301 ttattcattt taaaaaaatt attatttttg taaaactaat cccatagtca tactatcgtc
     6361 agaatatgct tcacccttaa cattactaaa aaactcaaca gacttaaatc caatattttc
     6421 aacttctaat aagaaacttt cttttgaaaa atagtgattc caaacataaa atacattttc
     6481 atctttttcg gttattacaa gagtttgttc taatgtatta taatcattgt atttacaatt
     6541 atcttgtaga cacatatatt tttcattact ccaaaatcct ccatcttcat ttatttccca
     6601 atatttaatt tcctcaaaat tattgtattt atttattgta aatacatcta atattaattt
     6661 tcctccaggt tttaaactat cataaatttt ttccattaat aaccttctat tttcagttga
     6721 caaagctcca taatcacaat aaattaaagt agccaaatca aattcatcat tatagttcat
     6781 atttaaataa ctttgaaaca agtaatttat gtttaaatta gtctccttat ttctattttg
     6841 tgcatagtta atagaacgtt ttgagaaatc aattccagtt accttataac ctttttgtgc
     6901 aaatttctct gcatataaac ctggaccaca acctaaatca agtaaattag gataattaac
     6961 tggatttgca actgtaacta tccaatttac agaatcctcg ataaagttaa aatttctact
     7021 tgctccttca aaattagtgt ctaaatgtgc ttttaataat tgttttgaaa tatactcatc
     7081 atcccaaaaa ttaatttcac tttgtttata aagctctggt ctttctaaat acatacataa
     7141 tttgtttaac aaaacatacc actccttaaa atttattttt aaaataaaaa aaccacagag
     7201 tttactctgt ggtttaactt caaaaagatg agtttaaata agagtaaaat agaaccaaat
     7261 atttttaagc acaacaataa aactatagct ttactttgtt tgcttattaa atttgatttt
     7321 attcactctt ttccagcaat acttatatca acgattttat gatatcaaat atcaccgaac
     7381 aacatcatcc tttcatattt ttattttatg aatcaattat atatttaaat tttatatttg
     7441 tcaataaatt ttaattttaa taaattttaa tcatcatagt aaaaaaaaat tttttatata
     7501 aaactaatca aaaaaagata tctgtccatc tcctgagttt tttaactttt ccttcattat
     7561 tttagagtaa ctgcttgata gagaatattt atatctaagt tcattaacca ttttatacaa
     7621 ttgattttta tactccctat taagtgaacc tgcactatat agtattgata atttatcaaa
     7681 taattcagga aactcttttt taacaaagtc aaaaaaaaca cctctagtta taccccttaa
     7741 gtataaagtc ccaggtaaaa cataatgtac attacattct tttgcatgtc taaatagcga
     7801 atttatatta tcaaaatcat ctgtaatata tggtataatt ggcataatat gtagaccaac
     7861 tgatgcgtta gtttttctaa attcttttaa cattttaaat cgtctcattg aatccacacc
     7921 atttggctca ataagccttt gtgtatcttc atttactgtg gtaattgtag atgctacatt
     7981 gatatatgta atcctagata gcttatcaat taaatcgtaa tctcttaaaa ttaaatctga
     8041 ctttgtagaa ataatagctg gtgtcttgta ttttatcagc aggtttaata tttctggcat
     8101 aattttatag tcagcttcta ttggttgata actatctgta actcctccta tattgattac
     8161 ttctttcttc catttactgc ttttaagttg tctctcaagt ttttctacta tatttgtttt
     8221 tacataaatc tcttcaaaat aattattgga gttaatgtac ttatgagagt aaatagcata
     8281 acaatattta catccatgct cacatccacg ataaatattt aaatcccatt tatatggcat
     8341 actccttttt aatttattac aagctatttc acaatttatt ggaatatatt ttattttatt
     8401 atacaaataa tcacctcttt atatacaata atactataat gttaatctat agtaattaaa
     8461 acatgatatt tccagaccac aattcataat ctttactata tttacaaagt aatatattcc
     8521 attcatttat tggtattcct ccacatgttg ctttttcttc taaattatat aagcattgct
     8581 ttaaatacat tttattatgc caatcatcat ataaattttc taaatttaaa ctatgttcat
     8641 tagatatatc atttgcaaaa tttctatttt tccattttaa tatacttgta tatttaagtt
     8701 cattaggttt ataatttctt tttaacattt catcatgtac aatttttgta taaaatttaa
     8761 attcatctat actataattc aatactttat tcactaatag atggtttgga gtaccttttt
     8821 tatctataga accttttata gcaagtagct ctctccattg acttactaat tgatttttag
     8881 gtaatacatc aattaaatcc ttatgccata atctcataat ttaactcctt actataaaat
     8941 attttataaa ttcaataacc aatctatctt acaattttac atgttcaata attttatctg
     9001 taaaaactat accatttaaa tggtctatct catgacaaag acattttgcc ataaaacctg
     9061 aacctttaat ttctattttc tcaccgtatt cgtttagagc ttgcactgta actttttttg
     9121 gtctttttaa tttaccccat acttctggaa aacttaaaca tccttcaaca acaatttgtt
     9181 ccccttcttg tttaataatt tcaggattaa caagcttaat aagtccttct cctaaatcta
     9241 taacaactaa tcgctttaat actcctactt ggcatgcagc taatcctcct ccatttggag
     9301 tattgtacat ggtctctgcc atgtcattta atatttctct tatcttatta tctacgtttt
     9361 ctacatactt actctttttt cttaaaattt catcatcaaa tgttcttatc tttcttatag
     9421 ccataatatt ctcccctact tatctgtgct cttgattttc tttcactttc ttcaaataaa
     9481 aatacacttt caattggttc attaaaaatt cttgaaatat catatgccaa ccatattgaa
     9541 ggttcatttt taccagtttc tatagcattt atagcctgtc tagatgttcc aactaatctt
     9601 cctagttgtt cttgtgtcaa tcctaatttt tctcttaata tctttatttt atttttcata
     9661 tttgctccat tcatgtaagg ttaactttac atcttaaatg taacattttt caacattact
     9721 gtcaagtcaa ccttacattt atatattaaa ttttcttatt taactatttt tttactaaac
     9781 tttttaaata ataataaaat ccatattaat gatataacaa ttgaaataaa catatagata
     9841 taattatctt ccttaaatgc cttcgatatc caaaaagttg gtaagaagaa cagaatatat
     9901 tgtgctttat ttaaaataaa aaatggtgct aaaataccca tcataaaaac acctgagaat
     9961 ttcgtaatta ccattccttc tagtttattt gatgataaag aaatcactag tattgatata
    10021 ataactcctt gtaataaaac taataaagaa attgataagt ttaacaagaa agaaatttta
    10081 gttagagaaa aaaagataag tagaatcata gtaataataa atgctaaggt acctggtatt
    10141 ccaaatcgtg aaataagata accagtttta ccaagcggag ttactattaa atatcttgaa
    10201 atgctgtcat caatttctcc caatatgacc attgtagaag caaaaaagaa cataaaaggt
    10261 gtaataaaag ctaaaagtaa atcaaacatc aagaaatacg gctctaaatt taattgataa
    10321 taaaacttgt taagtgatat attctgtatt aatggtattc caaattttat aaatactcca
    10381 cataatattg gagaaacaca tactataaaa agcatagcat ctctcttaat ttgttcaaac
    10441 tcttgtttaa acgcatttat aatctttatc atagttttac tccacctacc tttttccaac
    10501 tgctacaaat aaaataataa gttataaaat aaattattat tataattgat attaaaatat
    10561 taatcattat aaaattttta tctccactaa taagagaaat acatatatta aaagggtaca
    10621 aatttgcata tgattttgtg tcaagaagta cattgagtat tggtggaata aagcatataa
    10681 tctcaatagg aatacttaaa acaataaact gattcaaact agatgcttta cttgcaagta
    10741 tcaatccaag caaagaaaat ataatagaag aaaaaaaggt acctatagtt gatattataa
    10801 tattatctaa atttaatgta atagcaataa acatagctac aatactagat attactccta
    10861 atgaaattac ctttgataaa atatactcag atacttttac tggagataca gctaaggaat
    10921 ttaaaaccct ttgacttttc tcaagaagta caatagctcc cataaaaaac aatcccattg
    10981 ctgctgggtc agaataaatc ataattatag ctattttttc tctcataaaa gttggaaata
    11041 cattgataat acaaatatat ataatactta aaaaaagata taaaaaataa aaaccatact
    11101 taaattgaaa ctttatatct cctaaaataa gattttttag cctcattgta aattcctccc
    11161 tgttatctca ataaaaatat catttaatgt tggttcacta ctatgtattg ataaaagttt
    11221 atttttctta ataagcatat tcaaattttt atcattagca gtattgttca gaaaacattc
    11281 agaagttttt tctccattat caaagtaagt atatctaact ttaatagctc cttttgacat
    11341 aataagatta tgtggtgtat ctaaagcaga tattttacca tttacaatga acgctactct
    11401 gtcacaaagc tccgttgcat ctaacatgtt atgagttgtt aaaataatag ttttaccttt
    11461 agatttttct gataaaatta tatctttcat aaccttgcta tttgacgggt caagaccact
    11521 tgttggttca tctaaaaata aaatatctgg attatgtaat aaagctttta taaaatttaa
    11581 tcgagatttc ataccttttg aatactcaga taccctttta tttgcttcat tttctagtcc
    11641 tactgatttt aataactcat caatagatag tagtttttta gaatatagag acccaaaata
    11701 ttttaaattt tctattgcag tcaacttttc atataaacta ggaaattcaa aatcaactcc
    11761 tatattttca taaaatttat ttgagtgtct tttactctct actccattta caatgacact
    11821 tcctccataa ttagttatca ttccaattag aatcttttgt aatgtagatt ttcctgcacc
    11881 tgatggtcct aaaaatccta gtatttcccc tttttctact tcaaagttaa tattttgtaa
    11941 aaaatttcta tctgtataac taaatgataa atcatcaact ttaatcattc aatatcaccc
    12001 tttcaaattt ttgactattt tgactatatg gtcaataaaa tataattttt ttgtttgtta
    12061 ataattttta ataaaagtta ttagcaaaca aaatttaaat tctattccat aagttgtata
    12121 actaatccgt ttatcattaa tcttaaagca tcttcaaaac aattacttcc aacctcgcgc
    12181 ttatatagca tggtaagaaa aactactcta aatgctccac taaagctttc tatgtcttta
    12241 ttttttgcat ttggtatgca agatataatc tctccaattt caaagtcatc atgtctaaag
    12301 tgttcttcaa ttatagaatc tggtaacttt ctcaccaata tctcaaattc tccatttatc
    12361 atgagattta aaattgaagt actatcaacc atcctataga atttcataaa tatatctgtc
    12421 aattgttcac atgtaatact ttcatctact ctatttatct catataagat ttctttttgt
    12481 atagagtcat gtatatctct caatacctca aaaaagagca attcttttga ttgaaaaaat
    12541 agataaaaag ttccttttgg tatttttacc cgttttacaa gctcatcaac agtagttttt
    12601 ctaataccat attgctccat acacaatctt gcttcttctt taagtctttt tattatatac
    12661 tctctttctt tatcagaata cgatttaggc atctttttcc tccagtttga ctaaaataat
    12721 ttatatagtc aatataatac tttttctatc taatgtcaat agtattatta gtatatttaa
    12781 aaataagctt aaaaactttc gaaaattctg a
//
LOCUS       DAGQWR010000118        12741 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.118, whole genome shotgun sequence.
ACCESSION   DAGQWR010000118 DAGQWR010000000
VERSION     DAGQWR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.118"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(173..1423)
                     /locus_tag="MIW95_002932"
     CDS             complement(173..1423)
                     /locus_tag="MIW95_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBY3152243.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLYEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDSDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1455..2396)
                     /locus_tag="MIW95_002933"
     CDS             complement(1455..2396)
                     /locus_tag="MIW95_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBY3152244.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2636..3712)
                     /locus_tag="MIW95_002934"
     CDS             complement(2636..3712)
                     /locus_tag="MIW95_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBY3152245.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4085..4600)
                     /locus_tag="MIW95_002935"
     CDS             complement(4085..4600)
                     /locus_tag="MIW95_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBY3152246.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(4963..6033)
                     /gene="leuB"
                     /locus_tag="MIW95_002936"
     CDS             complement(4963..6033)
                     /gene="leuB"
                     /locus_tag="MIW95_002936"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBY3152247.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDEIGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(6040..6531)
                     /gene="leuD"
                     /locus_tag="MIW95_002937"
     CDS             complement(6040..6531)
                     /gene="leuD"
                     /locus_tag="MIW95_002937"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBY3152248.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKEKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6546..7823)
                     /gene="leuC"
                     /locus_tag="MIW95_002938"
     CDS             complement(6546..7823)
                     /gene="leuC"
                     /locus_tag="MIW95_002938"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBY3152249.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTITKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(7780..9522)
                     /gene="leuA"
                     /locus_tag="MIW95_002939"
     CDS             complement(7780..9522)
                     /gene="leuA"
                     /locus_tag="MIW95_002939"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBY3152250.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIKGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9646..9892)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10040..10486)
                     /locus_tag="MIW95_002940"
     CDS             complement(10040..10486)
                     /locus_tag="MIW95_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152251.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(10507..11226)
                     /locus_tag="MIW95_002941"
     CDS             complement(10507..11226)
                     /locus_tag="MIW95_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBY3152252.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYSGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGTRVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(11344..12618)
                     /locus_tag="MIW95_002942"
     CDS             complement(11344..12618)
                     /locus_tag="MIW95_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBY3152253.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
BASE COUNT         3848 a         2449 c         1313 g         5131 t
ORIGIN      
        1 tcctcaatta tagcatagaa tatgactatc tatatcctaa aattatccat ttctaaaaaa
       61 aggtaactat tattaagaat atcaacttat agaatttata ttccacatac gattttcaat
      121 caaaaaaaca agggctaaaa agtgaattgt acacttttta gccctttatt aattattcat
      181 ttatttcatg taagaatagc ccacatctta ctttaggttc aaaccatgtt gacttagctg
      241 gcattatctt atttgcatct gctactctta ttaaatcttc aatactagta ggatacattg
      301 caaatgctac tttcatatct tcattaactc ttttttctaa ttcttcaaga cctctaattc
      361 caccgataaa atcaattctt ttatctactc taggattgtc aattcctaaa atagcatcaa
      421 taacatagtc ttgtaatata gaaatgtcta atgagtcaac agggtcttct atttctaata
      481 aatttttatt tgctttcatt ttataccact tatcacctag gtacattccg aaagttcctt
      541 ttttgtcagg tttatatttt tcttttcctt ctatttcttc tagttcaaaa ttttctttta
      601 ttttatttat aaattcttcc tcgcttaaac catttaagtc tttgataacc ctattataat
      661 ccaaaaccat taaattctca tctggtgcaa tcattgctat gaagtaatta aattcttctt
      721 tgccagtata atttggattt ttagctctca tttctttggc tacattttct gctgaagctg
      781 tcctatgatg accatctgca atgtatagat aatctaattt ttcaaattta tctactaggt
      841 tatctattat atcatcacta tcaatcttcc atactgtatg ctctatcttg tcatctgtta
      901 tgaaatcata taaaggttca ttattatcca taaaatcatt gattatatca tctatcaccc
      961 tttgatgttt gtaagttaca agtatagttc ccgtattagc atcacagtat tttatgtgtt
     1021 tagttctatc aatttccttt tctggtcgag tgtactcatg tttttttatt gttccattca
     1081 agctttcatc aactgatata catgctacta taccaacttg agctctacca tccatgattt
     1141 gtttatatat atatatacac tctttgtcat cttttactag aatttcttgt tttcgaaact
     1201 catataaatt ttctcgcgct tttaaataaa ctttttcatc atataaatct atattttcat
     1261 ctaaatcaat ttctgattta tctatatgca aaaatgaata tggattccct tctgcaattc
     1321 ttctagcttc tttactattc ataacatcgt aaggaagtgc tgctacttta tctactatat
     1381 atttatttgg tctaatcgcc ttaaatggtt taatcgttgc cattactaat ttcctcctaa
     1441 ttatacaaaa tattttataa tgctacccct attagattgt tgaagtcaag aaagaaatct
     1501 tgaatcacat ttactatttc gtctcctatt ctttcttgag cttctactgt agatgcccca
     1561 atatgaggtg taggagaaac tcttggatga tttactaagt ctaaatttaa tgtaggttct
     1621 tgttcataaa catctatagc agctgctgat aatttacctt catctaatgc ttttagtaaa
     1681 tctttttcat caactaaacc gcctcttgca cagtttatta aataagcccc tttcttcatc
     1741 ttatttattt ctttttcagt tattactgcc cccttattct tatcaaatgg tatgtgtaaa
     1801 gataagaaat ctgcattttc taaaacttct tccatatcac agaatttata attgttaaaa
     1861 ccttgcgcct cacccattat gtcagtatat ataacattca tacctaataa ttctgctctc
     1921 tttgcaactt cttttgcaat tcttccaaaa cctataagtc caagtgtctt tccattaatt
     1981 tctgtacctt tatacttctt cttctcccat tttccatctc tcatagttac atttgcagta
     2041 tttatgaatc ttgcaagaac aaataattga cctattgtaa gctctgcaac agatatactg
     2101 cttgcatttg gagtattcat aacttttatg ccattagctt gagcatattt aacatctata
     2161 ttatccagac ctactccacc tcttactatc aacttcaact ttttaccctc taatgcttta
     2221 tctattacat ctttagtaac tttagtagct gaacgtacta caagtacatc aacatcttta
     2281 agtttatctc ctaaaacatc tttttcataa aactgctcta tcacattaaa atctaactct
     2341 ctcaattttc tcgctgcttc tttctctatt ccatcagtaa ctaatatatt atacataaca
     2401 aactcctcca atttttattc acttaatttt tccaattttt attttttaaa gtattttcat
     2461 caataaaaaa ctttacttaa tttaactttc tgtttatata attttcaaaa tattttttta
     2521 ttaaagtcat atcaaatttt cctattgttt gttaaaaatt taatatgtaa tttttataaa
     2581 atattattta ttatatattc taaatttatc ttaaaacata catattttta caatattatt
     2641 tattaaaacc taatatatca tcaatattat ctaataattc ttgtacatct tcgacagtat
     2701 agtcacccat atgtgaaatt ctaaaagttt tttcttttaa atctccatag ccatttgcta
     2761 tttggaatcc tcgttcttgt aattttgaat ttaaatttga tacactaata ccttgagtat
     2821 tttctataac agtaagtgta ttggataagt gattttcatt ggtaaaaatt tggaagtgtt
     2881 ttttagccca gtttctaact aagtttgcca tgtcttcatg tctgttgaat ctgttatcta
     2941 ggccttcatc taatatatta tctaattgga aatctaaagc gaacatgtga gaaagagatg
     3001 gtgttgaagg gtattggtag tttttcttga ttaagtattc atacattgct aacaaatcaa
     3061 gatatactcc tctaaaaggt acttgcttag ctctatcgat tgctttttga gaaaatgtgc
     3121 aaatagacat tcctggtggt aatcccagtg ctttttgtac tgatgttata caaatatcta
     3181 ttccaatctt atcaacctcg acttttattc caccggctga acttacagta tctacacagt
     3241 agattacatc ttcatacttt ttaacaactt ctccaatttc ttctattgga tttcttattc
     3301 cagttgaagt ttcattgtga gttacagtta ttaaatcgta ttctccggta gaaagcacct
     3361 tatcaaccat ttctggcgtt gttgcctctc ctaattcaac tttaaatata tccgctggga
     3421 cattattagc cactgccatt ttgtaccatc tatcaccaaa ggatccacag gaaaatactg
     3481 ctgccttttt ggatgtacag gaacgaatgg agccttccat taaaccagtt ccggatgacg
     3541 tggataaaag tatcgtgttg tttgtataga atagtttttg catcttttca cttatactct
     3601 tttggagcat agatgcatct tttcctctat gtcctatcat tggtgtagcc atcttttgca
     3661 agacttcttc tttaacatca ataggaccag gaataaataa tttcttactc attataattc
     3721 cccccttttt agtttgtaat atggatagcg tttttactgt ccttattttt aataactata
     3781 atatcaagct gaattaacta tgtcaacata aaatttattt tttatatata atttatttta
     3841 tatattttat gaaaacattc attttctgcc ttttcataag tctgaatttt ctgaattatt
     3901 ctctcaaaaa aaatatactt tttatctata taaattaatt tttttagtat gtaaatttga
     3961 cgctattacg ataaatcttt ttagtataac taatttattt ttatcttgta tttttattgt
     4021 atttttgaat tttatttttt ctaaatataa aatttatgtt ttaaaatata aaatttttat
     4081 ttaatcaaag ttcaatatat tccacttttt ctttcgattc ttcaaaaaac cattcttcat
     4141 tagaaaaatc accttctgca atttgtccaa taggcatttt catatacttt ccagttgctg
     4201 tcactgctat atctccattt ggtagaatta tttctcctgt accttcaaat aattttctgt
     4261 tttctttagt tattcttcca acaactttta tagtagcatc tgttggtact ggtttcttat
     4321 atttcaactc taaagatact gttactcccc atatttggtc atcatttata cttacagctc
     4381 ttccaatagt ctcatctaat attgcagcag acatacctcc atgaactctc ccaggataac
     4441 tttgatgcca atcctttgta ttacaaattg atacaagctc tccattttca agttcataaa
     4501 aatctgcttt aagtcctagc tcattttgtg ttccacaaac taaacaagat ttactactat
     4561 tttgcttctt caaaacttta tactttgcca aaaaatccat ctcctaaccg acattagtag
     4621 tcgcttaata ttaaataatt ctaaaatata ttatataata ttaataaaat ccctgcaaac
     4681 ttctaataat aaaaataggc tacttcaata tgataagttt aatttaaagt attattttcc
     4741 aaaaaataac tttatcaaaa tagaaactca aagtagatat aagcctcttt ttcgctatta
     4801 aaatagtaaa aatatatttg acttaatata aaaaagagtg tctcattaac taataaagtt
     4861 tattttgaga cacccctcat aaaacaaatt atactacaat agatttttac taattctaca
     4921 agttatatat ttaaattata ttattcaaat taaactataa ttctaaattt catttactat
     4981 taattcgccc attgctcttg ttcctacaac atttcccaca ccattataga tatcaatagt
     5041 tctataacct ttattgagta cactttgtac agcatcttca atacacttag cttcttcttc
     5101 taagttaaaa ctatgtctta acatcatgct tacagataga atagttgcaa ttggatttac
     5161 aatatctttt ccagctatat caggcgcact tccatgtatt ggttcataca taccaaaact
     5221 atcttctcta agtgatgctg atggtaacat acctatagaa cccgtaatca tagatgcttc
     5281 atcagatatt atatctccaa atatattatt agtcactatt acatcaaatt ggctagggtc
     5341 ttttacaact tgcatagcag cattatcaac atacatatgt gacagttcta catcctcaaa
     5401 ctctttagct aagtcttcca ttatacttct ccaaagtctt gaactctcaa gcacatttgc
     5461 cttatctaca cttataagtt ttttatttct atttctggct atttcaaaag ctctcttacc
     5521 aattcttctt atttcatttt cgttatatat ttctacatca taagcatttt cgataccatc
     5581 aatgattttt cttccacgtt ctccaaaata aatgccccct gttaattctc ttacaacaac
     5641 aaaatcaatg cctttctcaa ctatatccgt tctaagtggt gaagcttctt ttatactttc
     5701 atacattgta gctggtctta aatttgcaaa taaacccaat gactctctta attttaaaag
     5761 accagactct ggtcttttat ctcccttaca tttatcccac tttggtcctc caacagcacc
     5821 aagcaataca gcttcgtttt ttcgacaagt ttcaacagtt ttattaggta gaggttctcc
     5881 aatttcatca atcgcgcatc ctccagctaa tacatattca caatcgaatc tatgattaaa
     5941 tttacaacaa attttattta gaacctttat accttcatct ataatttccg gtccaacacc
     6001 atcacctttt atgactgcta tattacaatt catactccac taccccctat ttgcttttat
     6061 tgagttaatt aagccttcat ttaatataat attttgcata aactcaggaa atggttctgc
     6121 tttatattct ttttcttttg ttatattttt tatcacacct gtagaaaaat ctacttctat
     6181 attatcccct tcatctatgt tattggcagc ttcttcacat tcaagtatag gaagccctat
     6241 atttatagag tttctaaaaa atattctagc aaaagtagaa gcaatcacac aggatactcc
     6301 actttctttt attacaattg gtgcatgttc tcttgatgaa ccacaaccaa agtttttagt
     6361 tgcaactata atatctcctt tttttacctt agaaataaat ttatcatcaa tatcctccat
     6421 acaatgcgtt gctagttctt tataatcagc tatatttaaa tatctagctg gtattattac
     6481 atctgtatct atattgtcac cgaatttaaa aacacttcca ttggcaatca taaattttac
     6541 ctcccttaaa ttatttctga tggttttgat atttttccag ttatagctga tgctgctgct
     6601 acagctgggc ttgcaagata cacctctgaa tcaacatgcc ccattcttcc tacgaaattc
     6661 ctatttgttg ttgaaatgga tttttctcct gctgcaagta tacccatatg tccccctaga
     6721 catggaccac aagttggtgt agaaactgct gctcctgcct ctataaaagt ggtaatatac
     6781 cctttttcta tagcttcaag atatactttt tgagttccag gaaatatcat aactctaact
     6841 ccatctgcta cttttttgcc tttcattatc tcagctacta cttcaaggtc tgatattctt
     6901 ccatttgtac aagaacctat tacaacttgg tctatcttta tatccttatc tatctcatct
     6961 actgttctag tattttcagg aagatgagga aaggctactg tttcttttag ttcggctaaa
     7021 tctatttcgt atacttcatc atagactgca tcttcatctg cttcatatat atttacatct
     7081 tttgttatag ttgaacactt atgtgaattt atatattcca tagtcttatc gtctactgga
     7141 aagattccat tttttgctcc tgcttctatt gccatattac aaatagtaaa tctatcatcc
     7201 atagataagt attctactcc atctccacaa aattccatcg acttgtaaag tgctccatca
     7261 acacctatct cccctattat atgaagtatt atatctttac cacttatcca tttatttggt
     7321 ttattcttca aaacaaattt tattgctcca ggaaccttaa accaacattt tcctgtagcc
     7381 atacctgctc ccatatcagt tgaacctaca ccagtagaaa atgctcctaa tgctccatat
     7441 gtgcaagtat gagagtcagc acctataatt acatctccag cagttactat acctttttca
     7501 ggaagaagaa catgctctat tcccatatct cctacatcaa aaaaatttac aactcctttt
     7561 gattttgcga aacttcttgt aaatttacat tgttctgcac tctttatatc tttattaggt
     7621 gtaaagtggt ctaaaaccat cactacttta gtcttatcat aaacatcttc aattcctaat
     7681 ttttcaaatt ctctaatggc tactggtgat gttatatcat tacctaaaac tacatctaaa
     7741 tctgcttcga ttaattgccc tttcttaact tcatttaatt tagcatgttt ggctaatatt
     7801 ttctgcgtca tagtcatccc cattttttat ttcctcctca atataacttt tatttattgc
     7861 attcataagt gcattaatag ctgctgtatt tatgttttcc gatatactta caccaaaata
     7921 gacttcatta ttgtgctcta ttcctacata agctacccct cttgaatgtg acccttcctc
     7981 taatgcatgt tcccagtaat atctaaattt ataatctttc atgccacatt gctttagtgc
     8041 attattaaat gaatctacag gtccatttcc tataccaaca atcctttgtt catttcccat
     8101 ataacttatt cttgcagtca tctcaatcat gttagtatca ttagaatcat cattttcata
     8161 gtttaattca tccattgatt ttattttata cttcttaact ttataagggc tctcaacttc
     8221 aatatattct ttcttaaata aattaaatat tgcttcacta ctaagttctt ccccaattct
     8281 atcagattcc attttgacta catttccaaa atcagaatgc atattcttag gtatcataaa
     8341 tccataatct gtttccatta tataaacagc tcctcctttt ccagattggc tatttatcct
     8401 tataatttct ttgtattctc ttccaatatc atgagggtct attggtaaat atggaacttc
     8461 ccagtaatca ttatctctgt ttttcattgc tatcatgcct tttctaatag catcttgatg
     8521 tgaacctgaa aaagcacaat gtacaagatt acctgcatat ggatgtctat catgtaccat
     8581 aagttttgta cagtcttcat atataccaat tatcttatct atattgctaa aatctagctc
     8641 tgggtctatt ccttgtgtat aaagattaag tcccacattt actatatcca tatttccagt
     8701 tctttcacca tttccaaata gtgttccttc aagtctatcg gcaccagcca ataatccaag
     8761 ttcacttgca gccgtacaag ttcctctatc attgtgagta tgcaaactta gtataataga
     8821 atccctacaa cttatatttt tacaaaacca ttctatttgg tctgcatata cgtttggtgt
     8881 tgccatttcc actgttgatg gaagatttat tattactttt ttttgaggag ttggtttcca
     8941 aacatctatc actgcctcac atatttcaag tgcataatcc atctctgttc ctgtaaaact
     9001 ctctggtgaa tattcaaaag taaattctgt ttctggatat ttttttactt cttcattaaa
     9061 taattttgct ccttcaactg ctatatctat tatttcttgt ttactcatat taaaaaccac
     9121 atctctttga agaacagatg tagagttata taaatgtata attgcttttt tacatccctt
     9181 tattgattca aatgtttttt ctataagatg agccctagat tgtgtaagta cttgtatagt
     9241 tacatcatct ggaatcatat tctcatctat taattttctt aaaaatgtat actctgtatt
     9301 tgatgctgat ggaaatccta cctctatttc tttaaatcct gtatcaataa gcattttgaa
     9361 cattctgact ttttcattta cactcatagg cgttggcagt gactgatttc cgtctcttaa
     9421 atcgacacta caccaaattg gagccttaaa tatttcatta tctggccatt tacgatttac
     9481 tatttgcatt ttatcatatt ttttatactt tccacacttc atgttatttc cccctttttc
     9541 tgttttttgt attcagtata ccttatatcg acgcaatcct gcacacttca taaggataaa
     9601 tgtcaatttt atcattagaa taaaaaaaac ctcatctcta ataaaattag agacgaggtt
     9661 ttgctcgcgg taccactcta tttagctttc taaaaagctc ccttagtaat agccaataca
     9721 ataattataa tgactatatt tcctataacg tggaattact ccgattattc taagatttaa
     9781 atcttagaaa acagctccta gacaagttca gaactatcaa ctactaaatc acaccatcct
     9841 ttagctctct gaaagtttca ttgttcttac tacttctatt catcactttt ttaatcattt
     9901 aaattttcta aatattttga tttaatagtt attatataca cattaaaatg tattgtcaac
     9961 aaaaaaaata attatctcaa ttttttattt ttgataagca attctataaa acatgctaac
    10021 tattgaaaac agtatgatat tatgccttaa actttttcat cttttcatct atgatattca
    10081 ataatttttt ttcatcttcc aaactcaatg cccttaatct aattttataa ttagattcgt
    10141 cagtgtatat atttaatata ttttttacta cttctatttt ttctattatc ttataattaa
    10201 acttttgact ttgtttagat tttatactga atatacattg attatttatt aatattgaat
    10261 gattatcgag agtaatatat acttttccct catatttatt tgaaaaatca cgaccttgtt
    10321 taaatagaaa aactgctgtt attatacata aaatatataa tattgtcgtt gaatatataa
    10381 aaattgtagc taaaattata agtgctaaaa ccatatatat agtcattcct tttttaaaat
    10441 aaccattgtc ataataattc atatagataa acttattttt actcaaattt tttcactccc
    10501 aagattctaa aaatttatat caaaaaattc tataatttca tcttcatgat aatttatgac
    10561 taattcatct ggaaaatcta ctgcttctaa aactttttct gcattttcaa attctcctat
    10621 agaataacat atatgagagt cagtaccaag aattactctt gtaccatatg ttttacacaa
    10681 tgtcaacata tgacttacat tttcccaagt acccgttcta tgagagtttg aacttaatga
    10741 tgaattattt atttctaaaa ggatattttt atctttagct ttttttacta taggttcata
    10801 atctaaagga tacctactgt catctggatg tccaattatt tttaccttag gtttatccat
    10861 aacttttaaa atagcatttg tattttcttc ttttgtacca gaagctatac aaggtctatg
    10921 caaacttgcg attatataat ccaaatgttg caacatatct tcttgaacat ctaaattacc
    10981 actataatcc attatatttc cttctattcc cctaagaatc tttacacctt gcacttcacg
    11041 tggaagcaaa tgcaaattat aaaaatagaa tggatgtggt cctcctggca tattaggtcc
    11101 gtggtctgat attcctaagt attttatatt cttcttactc gcctcttcga cattttcctt
    11161 gaaagtacta tgtgcatgtc cacttattag agtgtgacaa tgtacatcca taatagactt
    11221 tttcattaaa tttcctccta atattttaca taaattcgtg aaaatagcta aagggctatt
    11281 tcaaaactaa agctgtcata atagacagtg taacgtttta aaacagcccc attttcaatt
    11341 attttacact tctaaatctt taaatttatc tttaaataat tctccaatac ttcctaaaga
    11401 ttcctctact tctaggtatt ctgttatatc ctcttctggc tctctagttg cttcttttat
    11461 tgaaaccaac attcttttag tctttggttt aaaatcaaga atcttaactt taacctcatc
    11521 tccaatatta acaacattag taactttagc cactctagta tcactcaatt cagatattgg
    11581 taaatacgca tctacacctt ctttaatttg aacaaaagcc cctttttcta ttattctaag
    11641 aactttagca ttgacaacat catcaatttg tacttctttt gctattaaat cccaagggtc
    11701 attatttatg tcttttaatg ccaaggcaat ttttttagtc tctttattta catccaacac
    11761 ataaacttct acttcttgtc cctctgaaac aacatcttca acactttcaa ctcttgtcca
    11821 agctaaatta tttatatgtg ctaatccttc aatcccacca atatccacaa atgcaccata
    11881 aggcattatc tttgtcacct taccatctct tttttgtcca acttcaagag aattaaataa
    11941 gtcttcttta gctgctttga tacgagcttt ttcttcttct ctttcttgct taattcttgc
    12001 tctttcagct tctttttctg ccttaatttg agctcttctt tcttctctct ctttatttat
    12061 acgctcttgt aatacatctc tatgagatgc tacaagtcta ttttttctaa catccaattc
    12121 ttttatataa acttctaagt taagaccaac aaatttagat gtatctgtaa taaattttgt
    12181 atcaagttga gaaattggta taaaaaatgt atatgtattg taattagcaa atacaccttt
    12241 atctatattc ttttcaacat ttacagttat tattctatgt tcatcataag ccttttgtaa
    12301 ctcggcaaaa tcatttcttt tgtcaagttg taatttagat aaagttattg taccatcttt
    12361 ttgagatact tttgttatta cagcagttat ttcatcacct atgtggtata tatcttctat
    12421 atattgattt ttttctatgt tcaattcact tataggtatt attccatcaa acccatagtc
    12481 taatcctaat tgtacttcat catttctcac tgcagttatt tttccagttg tcaattctcc
    12541 aactttcact ttgctgaata cttgttcttg ctggtcaagt aattcttgca ttgttaaatc
    12601 attttccatt ataaacatcc tttcctctta ttgattatca tatctgtaaa tattatacca
    12661 ttaatattgc aaatacttac atttttctta ctataatttt taaaataaat tcaacttcat
    12721 atatttatac attatagaaa a
//
LOCUS       DAGQWR010000119        12715 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.119, whole genome shotgun sequence.
ACCESSION   DAGQWR010000119 DAGQWR010000000
VERSION     DAGQWR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12715
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.119"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(181..870)
                     /locus_tag="MIW95_002943"
     CDS             complement(181..870)
                     /locus_tag="MIW95_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBY3152254.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            complement(863..1234)
                     /locus_tag="MIW95_002944"
     CDS             complement(863..1234)
                     /locus_tag="MIW95_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBY3152255.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            complement(1416..2762)
                     /locus_tag="MIW95_002945"
     CDS             complement(1416..2762)
                     /locus_tag="MIW95_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBY3152256.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIRKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            complement(2944..3537)
                     /locus_tag="MIW95_002946"
     CDS             complement(2944..3537)
                     /locus_tag="MIW95_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBY3152257.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(3602..5071)
                     /locus_tag="MIW95_002947"
     CDS             complement(3602..5071)
                     /locus_tag="MIW95_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBY3152258.1"
                     /translation="MSNVNKKLVIAIGAVALVAIIFIGIMCMQFKGEKIAKNTYVNGV
                     DIGKLTKSQAKQELAKKYKLENVEFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SSFFGNLSKTISTNFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLTNIDTNLGTY
                     STIFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(5186..6577)
                     /locus_tag="MIW95_002948"
     CDS             complement(5186..6577)
                     /locus_tag="MIW95_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBY3152259.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKEDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(6743..6991)
                     /locus_tag="MIW95_002949"
     CDS             complement(6743..6991)
                     /locus_tag="MIW95_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152260.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSTNKKQKSHSRDIKRKIS"
     gene            7257..9386
                     /locus_tag="MIW95_002950"
     CDS             7257..9386
                     /locus_tag="MIW95_002950"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBY3152261.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            complement(9643..10242)
                     /locus_tag="MIW95_002951"
     CDS             complement(9643..10242)
                     /locus_tag="MIW95_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBY3152262.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(10260..>12715)
                     /locus_tag="MIW95_002952"
     CDS             complement(10260..>12715)
                     /locus_tag="MIW95_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBY3152263.1"
                     /translation="FDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEY
                     RPGQYELTKTIRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLIST
                     DTKELQIQLINKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTK
                     GEILSLIFLKRLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDC
                     LYKNRIEELKEEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINS
                     KSLRYLLQEIYPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSIN
                     LIIEEIDSILKFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEK
                     IKLSCEKIIKNLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEY
                     DEKDDYARIVEIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSY
                     FKNTLGIDRVKNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKIT
                     EGHTLALFNSKDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCF
                     EGVDIPGDGLVCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRI
                     LRSKYDYGCFVIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLIL
                     KSVIFDTIKALNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKY
                     FDRNYLINLDIFMREEDKN"
BASE COUNT         4105 a         2090 c         1298 g         5222 t
ORIGIN      
        1 aattcaagtt tagtaattat ctctttataa ttaattccta aatgaaattt ttctttttat
       61 aatttcagta catgattttt ttatttcatt atttgcattt ttttcgttca gtttttattt
      121 taaatttttt ttattttatt ttgttttaag tattcaaggt actcgtctta attttatttt
      181 ttatataaaa ttcattataa ttggaattag tattactgta attgtaccag aaactccaat
      241 agaaacacca ctcatagctc cttctatttc tccaagttca aaagcttttg tagttcctaa
      301 agcatgtgca gatgttccaa gagcaattcc tttagcaact ggatgattta tattccccag
      361 tttgaatact gtagaagcaa ccataccacc aaaaatacct gtaaaaatta tggctacaac
      421 tgttattgat tctataccat ttaaagaatt tgctaaagat attcccattg gtgttgttat
      481 agattttgga attagtgaat ttattatact tacatctgaa ttagctattt tgccaattat
      541 caatacagat acaaaagaaa tcactatacc acaacctatt cctacaataa tctcaaacat
      601 atgcttttta aataattcaa attgtttata aagtggaact gcaagtacta ctgtgacagg
      661 acctaaaaag aaatttagac tatcagctcc tatcttgtaa ttctcataag ggattttaga
      721 tatgcttaaa aacaatatta ttcctacgat tgcaattaat aaagggttac atataggatt
      781 acttgttttt cgttgaatgt atcttcctaa attgaaaaaa actattgtta ctattatacc
      841 aaatactggt gtctgtaata cattatacat ttttttgtcc ttcttccttc ttttttatca
      901 ttttctccac aataattcca gtagtataca ttactattag tgtacttaaa catatgacta
      961 ataaaagcac taatacattt tcccttatta ggtctaatga ctttataagt gatacacctg
     1021 ctggaataaa aaatatagcc atattatcga ggaaaaatcc acttacattt tctatactac
     1081 tcaagtctaa aactttaaat tgtagtagta gaaataataa tagcatacca actatactcc
     1141 caggaataag tataattcct tgtataaagg aacttatata ctctcctatt acccagatag
     1201 ataaaattat tactaattga ttgaaaactt tcattttcca cctccaaaaa ttaaagagat
     1261 tgcttcaaat catatatttg tagttttatc aacaagcaaa tatctttctt taaaatagaa
     1321 agttataatt tgacacaatc tctttttaaa tttattttta ttttaattta ataaaataca
     1381 tacttggttg tttcaaaaaa cttaactata taaacctatt tttcacttaa gatttctttt
     1441 atttttgaat tgttatacat actgtctgga acttcacttc tcaaactata aaaagtctgt
     1501 tgtgcatttt ttaaatcgcc attatcataa taaagcatac ccaactcgta atatgaatca
     1561 tcataatact ggtcattttt agtataagta tttatatatt ttttgtaata ttttatagct
     1621 tcatccttat ttcctaataa tttatttgtt attgctaatt gatatattga ctcagaaata
     1681 tattttttag ttttaccaga agaaactaca cttttaaaat tatctatggc tttttcatat
     1741 tctttgtttt tctttaaatt tagagcttga taatataaat cttcctcatc agctacagtt
     1801 atctctcttt ctggttcttt atttttaacc tcatttaatt ccttattcgt tttttctaat
     1861 tttttactcg tttctcctaa ctttttatca gtttcactta acttttcctc tttactactt
     1921 atttggttat tcaagttttc tattctcttt ttgctttgaa tcaaatatat ccccatacct
     1981 attaaaacac agccaactat aatatatggt aaaatacttt tctttctttt tacttttctt
     2041 atttcttcct ttttacttgt atcttctttt tcttcttcat ttttttcaaa tacaggatta
     2101 tctttatcta gctctttaag tttgtctaaa tatccttctc tcttttttac attacctagt
     2161 ttatcatata acaaagataa gattacatat ggctctatta gctcaggctc ttcttcaatt
     2221 atgttctcta aaatatgtat tgactcataa tttaattctt catttataaa tcttatagaa
     2281 tgattgtatc ttcctaaaaa agttttaaaa tcttcagatg aaattagatt tacatatttt
     2341 ctagatatat gattattctc ataacataaa gcagtataaa aactctcaaa tgatttagca
     2401 aaatcacatt ttaataactt taaaagacct ctaagattta gtatatcaat atctttagga
     2461 tttatctcca gagctttatt aaccatatta atggcactag ttatataatt tttatatgct
     2521 aaatccaatg cattgttata tcttttatat gattctaatt ttaactcctt agacgttgtg
     2581 tctgactttg aatttaaagc tccttgcaag tttaatagag tttcttcttt ataatttgta
     2641 gtcacataac tctgttttaa tgcttttaat gtttcatcac cgtcttcttc ttctatatta
     2701 aaaaaagtca aatcactttt attatcttct atcaaagata tatctaaaat ttttctaggc
     2761 atgctttcta tgtcttgttt tatatcttta actaaaataa tactcacctc cactctaaaa
     2821 tatatattta agataacata tttaatatgt caagtcaaaa ctatacatat ttttccacca
     2881 ttctcataat aaaaaaggta atcctcttct tttcataaaa aggttacctc tttttcaata
     2941 ctattaactt ttaatgttga atttttttac ttttctatag agtgtagcta tacctatatt
     3001 taatgcctca gctacttttt tcataccttc tgtattactt ccatatttct ttatggcatt
     3061 ttctatagct tccctctcaa ggtcatctat agtcttaact tcttgtttat aaagttcatc
     3121 tagtttaaaa gatattaaat cagacatact ccttacaaaa gcagttaact ccttatcttt
     3181 tgaaagtatc ttttcttttt gctcttcatt aaacgcagct atacccaaga ttccaagtat
     3241 atgttctccc attttaacag gagtacatat atcagctaat tctttacaat tttctctatt
     3301 tgcacattta ttacagttct cttctatttc cttatttata acataggatt ttccatcttt
     3361 taaaacaaga gagtacactg acttctctga tattgtctct cctattttac ctctgaatat
     3421 tcctgtacct gctatcctag ttaaatcttt tgtaactaaa gtaacatcta catccaatac
     3481 attagaaata ttctcgcaaa tactttgaac gtaattcgtt atattattta aattcaaagt
     3541 aatccctcct atttagattt atatttttta ttactctaca taaatcctat tcttgagaac
     3601 tttaagcttg cggttgtggt tgtgtctcat taggcttatt atcagttggt ggtgtttctg
     3661 gtttttgttg attatttgga ttttgattgt ttgtattttg attattctta ttttcgtctg
     3721 tatttggttt ttcctctggt tttggttcca tagtcccaac taatataata ccttccttct
     3781 taggataata tgaattagct attttttcag ttttaatttt tttaccattt gcatcattaa
     3841 atgttctata tgttgataca gaatatgcat ttcttccact tttgtctact ttttctttac
     3901 ctttaggcat tgtagggtca ttaactcttt tcattgtagt ttgtgaaacg ccatctaatt
     3961 ttgttgatat cgttatattt tgtttatctt ctgcacttcc atatatattg caaacaattt
     4021 gatttcctga aacgtaattt tttatgtaaa ctggatgctt tagattattt ttaaataaga
     4081 aatctattcc actatctgca acagttgcat ctcttccttt tggaacataa cttgatggta
     4141 tagtatgatt ttttacatta acaatttcta aacctgcgta taacacagaa ttgtataacg
     4201 tagatgaaac ttgacaaact cctccaccat aatcttcttc catctcacct tccattatta
     4261 caggtgcact tttatatcca ttttccttac ttcttttccc tgtatgttta ttaaatgaga
     4321 aagtttctcc tggcataagt aatacattac ttatattatc tgttgctaat tttatgttta
     4381 tacttctatt tatttgagaa gttttaaata ttgttgaata cgtacctaaa tttgtatcta
     4441 tattagtaag ttgctctttt tttacttttg ctggagtagc ttttactact aactcatttt
     4501 tatagttacc ttttttagtt tgattatcaa aattctccat actcttagaa atatccattt
     4561 taagaccatc agaatctggt ataaccttga ctttttcacc acttatatca agtgtagcat
     4621 cttttacatc tacatctatt tctttagcta ttttttccat ttctgctttt aatttatttt
     4681 tatcataatt tatcacaact actaagttgc tttttttgcc aaaatttgtt gaaatagttt
     4741 ttgatagatt gccaaagaaa ctactctttc tatttaaatt atatgcgttt tctactgttt
     4801 tatccagatc agtagataaa tttaaatctt tgctctttac tttccaactt ttatcattgt
     4861 aattaaactc aacattctct aatttgtatt ttttagctaa ttcctgtttt gcttgcgatt
     4921 tagttaattt tccaatatct actccattaa catatgtgtt ttttgctatt ttctcccctt
     4981 taaattgcat gcacattatg cctataaaaa ttattgctac taatgctaca gctccaatag
     5041 ctataactag ctttttgttc acattactca tcccatactt ctccttacaa taaaattatt
     5101 tatatatccc tattattata ttactacata atgcgcctta aaaccccaaa aactttttaa
     5161 ttatatatta atttaatatt attaattaat attcatttag tccattttct gatatggcat
     5221 atattttttc attgtaaata ttaatagcat aaacttcttt gcctctaaat aagcctttat
     5281 ttactacagc ataataataa atatttttat attttccaaa caattcttct aaactctttt
     5341 tactttttac atatctatct gctttctcta tatctaatat ctcaatcttt ttatacttat
     5401 tttctttttc atcaagagta ttagttaaca caaacttagc tttatctttt gtaataccaa
     5461 attgataact tccatcttct aatattctaa agtatccatc accataatct actttgtaat
     5521 cttttttacc atctttatac gaatataaat ctttagtttt tgcacttacc aaaacttcat
     5581 ttattaattt atcatactca tatacttcag cataatagta tttattattt tttatcattt
     5641 gtggaagtga atttatagat agatttgcct tgtctttgcc acttttttta gctattacat
     5701 ttataatgtc atcagatgtt aaggtataca atgataaatt ctttttatat tttttttcta
     5761 aactctgtac cttttcatca tctgaataat cttcttttaa atactcaata tattgtaatg
     5821 ctctttcata atctccagaa gtatttgcct cttcagctct acttatatag taatcataca
     5881 tttcttcaat acattgttct ttcccatttt gagccatact gtaatacttt tcatcttctt
     5941 ttaatacttt attatacttt tcaatagctt cataatactt atatgctttt tggtctttta
     6001 ctgctgagtc ataaacatct ctagactctt tgattgtttc tatattctgt ttgtatacat
     6061 ctaggttact acccatacca tttaatattg aaacagtact tatgtagtta atggcaatat
     6121 cataagtagt attttcttct ttatacattt ctctaactct atcagcttga gctaataagt
     6181 catccacatc tatacttatt ttgtttaatt ctttcacagt atttattaat ccaataaaac
     6241 tttcttgact tatgtctcca tctaaatatt tatcaccact atttaatagc aatttattta
     6301 tttttttaga aacagactta ttaaaccttt ccatttttga atcagaaaac tttttttcta
     6361 tacttgtgta gtaatcaaca gctatagaat actgtctttt ctctataatc tttaacaact
     6421 cattagcttc atttcctaca ctaaactcgt tactacttaa gaaaaatact attatcaaag
     6481 caattacacc taatgttgat aatgaaatcg caactataga tttccttttt tcatcaataa
     6541 aatctacagc cttctcaaat aaatctctaa aattcattat agttccctct ccatttaata
     6601 atctttatat tttatggtaa actaaatttc ttattggtta ttatatcata attcttgaaa
     6661 aaaaataaaa acacaagtta aatcgtgttt ttattttacc tatcaatcat tattttatac
     6721 ataaatattt cctttacata aattatgata tctttctttt tatatcccta gaatgactct
     6781 tttgtttttt attagtactg acagttttcg ttcttgtctt gtgtatagct ttgctatcaa
     6841 atatttttct agcattgcat aaaacaattc caattgttaa acaaactgta agcacacttc
     6901 ccatcaaaaa gtaataatcc aatcctatat gtactcccat aactgaattt attaacacta
     6961 atgcaaaagt cattgcaaaa catatcatca tggagataac taaaattctt agaatttttt
     7021 tcaatccaat cacctcgtat aatcactaaa attgacacta ccccttatcc attactatac
     7081 caaattagta aaaaaatagc aattactatt gtcatttata ttaggaaaat attaaatctt
     7141 tttaataaat atcttttttt gacataagga ttacttaact tttaataaaa tagtaacact
     7201 aatatgtatt aattaataga ataaaataaa tgataattta ttgaggggtg atttacatgc
     7261 caaatttgcc aagtttaggg tcaaaggctc ctgattttaa agccaataca acaaatggtc
     7321 ctattagact ctctgactat aagggtaatt ggattgtttt attttcacat cctggtgatt
     7381 ttacaccagt ttgtactaca gaatttttat gttttgctaa atattatgac gaatttaaaa
     7441 aaagaaatac agaactaatt ggtctaagtg ttgatagtaa cagttcacat ttagcttgga
     7501 tgtataatat ttctttactt acaggtgtag aaattccatt tcctattata gaagatagag
     7561 atatgagaat tgccaagtta tacggcatga tatcaaaacc aatgagtgat acatcaactg
     7621 ttcgctctgt atttattata gataataatc aaattctaag aacgattctt tattatccac
     7681 taactacagg aagaaatatt ccagaaatac ttagaatagt agatgcactt cagactagtg
     7741 atagagataa tatagttact cctgcaaact ggtttcctgg aatgccagtg attttacctt
     7801 atcctaaaaa ctataaggaa ttaaaaaata gagttaacag ttgtaataag aaatattcat
     7861 gtatggactg gtacttatgt tttgtaccag ataattataa tgatgaagaa gtgagcaaga
     7921 aaattgataa tacttgtagc tggaaaaaag aacatactaa aaacattgaa aatgaatgta
     7981 actgtgaaca tgaacatcat gactacctga acaaagctct tgactgtaaa caagaacaca
     8041 agactgatat taaagatgat tgcaaccatg agaaaaaaca tactaaaaat actaacaaag
     8101 ttcacaactc caaacaagat aagtttaaag ataagtcttg tgatgaaatg aattttaact
     8161 atgataaaga tgaatcttgc gacaaaataa attctagcta taacaaagaa gatagtagtt
     8221 atgaagattt ctataaacat aattataaaa actacgatta tactagcgaa aaaaatacta
     8281 aaaaaatagc tatgaaaact ttaaaagatt caaaaaaatt agttagacca caaataacag
     8341 acccatacaa tccaatagtt gaaaatgcaa actgtccaga tataaatcca attgtagcag
     8401 aatatgttct tggaaatcca actaatgtag atgctcaact attagatgca gttatatttg
     8461 cttttgctga gatagaccag tctggaaatt tgtttattcc ttatcctaga tttttaaatc
     8521 aattacttgc tcttaaaggt gaaaaaccta gcttaaaagt aattgtagct attggaggtt
     8581 ggggagctga aggattctct gatgcagcat taacacctac atctagatat aattttgcaa
     8641 gacaggtcaa tcagatgata aatgaatatg ctttagatgg aatagatata gactgggaat
     8701 atcctggaag tagtgcatct ggaataacat caagacctca agatagagaa aactttacac
     8761 tcttactaac tgccataaga gatgttatag gagatgataa atggcttagt gtagctggaa
     8821 caggagatag aggatatata aattcaagtg ctgaaataga taaaatagct cctataatag
     8881 attattttaa tcttatgagt tatgatttta cagcaggtga aacaggccca aatggtagaa
     8941 aacatcaagc aaatcttttt gattcagact tatctttgcc aggatatagt gttgatgcaa
     9001 tggtgagaaa tcttgagaat gctggaatgc cttctgaaaa aatccttctc ggtataccat
     9061 tttatggaag attaggtgcc actataacaa gaacttatga tgagcttaga agggattata
     9121 taaataaaaa tggatatgaa tatagatttg ataatactgc tcaagttccg tatttagtta
     9181 aggatggaga ttttgcaatg tcatatgatg atgctttatc aatattctta aaaactcaat
     9241 atgttcttag aaattgtcta ggtggtgtat tctcatggac atcaacttat gaccaagcaa
     9301 atatactggc tagaaccatg tctattggta taaatgaccc tgaagtatta aaagaagaac
     9361 ttgaaggtat ttatgggcaa ttctaataga atgtaggaag attttatagt tatataaaaa
     9421 gcagatttta ttatattaaa taagttgtat ctaattaata ctattattaa ttgtttataa
     9481 attagatact tattttttca taatacaatc tgctttttta atcaaaattt atttaattat
     9541 aagttgttta attacaagtt gtttaattac aagttgttta attataaagt aggaatttag
     9601 atatactctt ataccatgta attatgtata aatatttttt tattatacac caatagcttt
     9661 tttagctaat ttatcagctt cctcattata cttatttcca gaatgagctt taacctttgt
     9721 aaattttaca ttaagcttat tcttaatact gtcatagaaa tttttatatg ctatagttcc
     9781 ttctttattt gttttccaaa ctccagtaca ccacttttca atcccttcat agtcaaagta
     9841 taaaatcaag ttttgaacat tattgtcaat acagtactgc attgcaattt tagaagcctc
     9901 tatctctcca gctacatttc tcatacttac taaagttttc tcttttcctt tttcactata
     9961 agtttttata acttcattat tttttaaaat cacaacaccc gaaccataca tttttactga
    10021 atgttcataa cttccatcta catatgcttc tataaactcc ttatcttctt gaaaactcaa
    10081 tttttctgac ccatcaatat attctttagc ttcttgaaaa gtagaaaaac tcttatattc
    10141 agctcctgag aatccattta cttgtttttt gcactcatcc catgtattat atacacctat
    10201 atttttacct tttttcacag cataaaactt ttgttttgcc aaatatctca ctcccactct
    10261 taatttttat cttcttctct cataaatata tctaaattta taagatagtt tctatcaaaa
    10321 tattttactt tcatatcttt ttttataata tcaatatggt acacttctcc ttttacagct
    10381 ctttgtctta tattttcatt tatatctttt tttataatat ctttatcaac atcattgtta
    10441 tccattttag catcaacatt caacgctttt atagtatcaa atataacaga ttttagtatt
    10501 aaacttctag atttatttaa atgtcgcctt atataagtat aaaactcatt gctattaacc
    10561 tttgaaattt tacaatcgtg taaatctttc tctaatctct ttaaaacact tatattagtt
    10621 cctacatcaa aaatcacaaa acatccatag tcatatttgc ttcttaaaat tctccccata
    10681 gcttgcttta cctttatggt catttgagga taatttatag tatagtagtc tataccatac
    10741 ttcttcatta tagtaaaata caaagggtct tttggattta gattcggaag cttgtctaaa
    10801 gttacacaaa ccaaaccatc ccctggaatg tctacgcctt caaaacatcc ttttgagcca
    10861 agtactacac attttctatt taaatcgtta aggtgtctaa tccccttttt atcagcataa
    10921 atttctaagt taaaactatg taagtatttt ttcaatattt cataagtttt ttcttgtcta
    10981 tctttactat taaaaagagc taatgtatga ccttctgtaa tctttgatat attagatata
    11041 attttactca tctcatttgg aaattcagaa tttctgtact cacaaatatc agaaaatcca
    11101 actacagaga ctctattttt ataatcaaaa attggttcta taatcttttc cacattctta
    11161 actctgtcta ttcctaatgt atttttaaaa taagacatac tttcagataa actcaaagtt
    11221 gctgataaaa atattccttt ttctaattga gataaaatat tttcttcaaa taaatctgct
    11281 atttttaatg gtactactcg aaattcaaag tcattataat tcttatcaat ttcaactatc
    11341 cttgcataat catccttttc atcatactct aaaaagattt caaatataat ctttatatcc
    11401 tctaaatctc ttgtctttgc ctttccaaat ttatatatat ctgcttcttt atctatacta
    11461 tcatcatcaa tattcctata aattattatt attattgaaa ctaaattttt tattattttc
    11521 tcacaactca actttatctt ttcactatag gctctataac ttatttcagt atctatacca
    11581 tcctttttta gttttcccac aatttcatct atttgcaaat tcagttccca tcttaaatta
    11641 taattactta cattattata ttcattacaa ttaccaaact ttaatattga atctatctct
    11701 tcaataatga gatttatact tctagcaatt ttttgtttat cttctcttcc aatttttaat
    11761 acattataaa atttatcaaa agcctttatt tttctcatat ttctactata ttttttatat
    11821 ataaagctac tattttgaat aaactcgtaa ggataaattt cttgtaataa ataccttaaa
    11881 gactttgaat ttataatgct tgaaaagaaa tcatatcctt tttcagtcaa attatgtgct
    11941 tcatctacta ttatattttc taaaggtttt tcatccttat acggccattt agctaatagt
    12001 gaatgattta ctactgttat atgttcttct tttaattctt ctattctgtt tttatataaa
    12061 caatcttttt tgcatttttt aggtctgcac atatttgggt cacaacttac atttcttaag
    12121 tgggtactta tttccttgaa gttgtcaaac actaaataat ttatttcttc tatatctcca
    12181 tacttgccac cttcaactaa tctcttcaaa aatattaaag ataaaatttc tcctttagta
    12241 gggttttgag actcataatc atctatatat gcttccagtc tatctataca aatatagtta
    12301 ttttttccct ttatatatcc gtaacttact tttccattta gacccaaaga atctaacaca
    12361 tttggaatat ctttatttat taattgtatc tgcaattcct ttgtgtcagt agaaattaat
    12421 aatctttttt tattaattct agcctctaat attgcaggca ataaatatcc tacactttta
    12481 cctataccag ttggtgcctc tatacatgca attttttctt catcttcgct atttctaaac
    12541 aactctctta ttgttttagt aagctcatat tgaccaggtc tatattcata tgtaaatcct
    12601 tctttattct cccaaatagt tttgtatttt aataattctt cataattttt ttcttcttca
    12661 tttaaaattt ttaaggtttc tctttccttc ttcttttctt cttttgagtc aaata
//
LOCUS       DAGQWR010000120        12544 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.120, whole genome shotgun sequence.
ACCESSION   DAGQWR010000120 DAGQWR010000000
VERSION     DAGQWR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.120"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            194..844
                     /locus_tag="MIW95_002953"
     CDS             194..844
                     /locus_tag="MIW95_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBY3152264.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(984..2360)
                     /locus_tag="MIW95_002954"
     CDS             complement(984..2360)
                     /locus_tag="MIW95_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY3152265.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2376..4289)
                     /locus_tag="MIW95_002955"
     CDS             complement(2376..4289)
                     /locus_tag="MIW95_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBY3152266.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4425..5057
                     /locus_tag="MIW95_002956"
     CDS             4425..5057
                     /locus_tag="MIW95_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBY3152267.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5196..5735
                     /locus_tag="MIW95_002957"
     CDS             5196..5735
                     /locus_tag="MIW95_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBY3152268.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAARKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(5911..6081)
                     /locus_tag="MIW95_002958"
     CDS             complement(5911..6081)
                     /locus_tag="MIW95_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY3152269.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            6366..7433
                     /locus_tag="MIW95_002959"
     CDS             6366..7433
                     /locus_tag="MIW95_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBY3152270.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSIEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSSSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            7603..9504
                     /gene="cooS"
                     /locus_tag="MIW95_002960"
     CDS             7603..9504
                     /gene="cooS"
                     /locus_tag="MIW95_002960"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBY3152271.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            9497..9970
                     /locus_tag="MIW95_002961"
     CDS             9497..9970
                     /locus_tag="MIW95_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY3152272.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            9945..11171
                     /locus_tag="MIW95_002962"
     CDS             9945..11171
                     /locus_tag="MIW95_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBY3152273.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSV"
     gene            complement(11310..12497)
                     /locus_tag="MIW95_002963"
     CDS             complement(11310..12497)
                     /locus_tag="MIW95_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY3152274.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKSFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
BASE COUNT         4730 a         1595 c         2009 g         4210 t
ORIGIN      
        1 taattttgta aatatagaat tatattaaaa taaattatat atgaaaatat atatgtataa
       61 gtttattctg cgtgagtaga atgaatttgt caagtttaat ttagttgtaa ttgtatcttt
      121 tattaaaaat aatataaatt aaaacatata aataaaatat tttatacaat atattgtaaa
      181 ggaggtaact ttgatgaaag cagaaaaaac aagtatatat ccaattatta aaaataattt
      241 aaacaaaata tcaattgttg aatataaaaa gggtcaaaga tttgtagcat cagataaaga
      301 cttacaagaa gtgttcttta taatagaggg agttgcatta gtggagtgta ctactagagg
      361 aggaaataaa tttcttgttg acattgtacc tgaaaatgaa tttataggca aaatcagtta
      421 catatatgaa cataatttga aatgtgatat atttgcaaag accaatataa aattatttcg
      481 atttgaaaaa aatgtatttg aagagtttta tctcaaacca gattttattg cactatttca
      541 cagaaaatgt acaagaagaa tatatgaatt atataaaacc agaatggtaa gagaactatt
      601 ttcttgcaca gaagttattg cctattgtat atcaaataaa caagaaaata atgtctgcaa
      661 tataaaagct attcgtaatt tcagtgaaat ctattcaatt agtagaaaaa gtagatacca
      721 ttctctgcta aagttaactg aaaaggagat agtaaaaaaa atgggtaata catatgaaat
      781 tttaaactat gatgaattac ataaattggc atttgaagta aaagagtttt tggaagatga
      841 gtaaaaattg ttacttaatc aaaataaagt ataataattt ctgtaaaaaa tagaaatagt
      901 ctcactgaaa atcaaaaata ctttagttat tttcaatgag actgtttaga aatattgtat
      961 acttataatg tatagtataa ttattaatca atttccttta ctatattaat agcccatttt
     1021 ttagataatg ctctaggaac accaaatata aacttaacca cttcttcact ataagtgagt
     1081 aaaacgagta caaatatagg tgcaccttta aatgcagcta taaaagttag aggaatagaa
     1141 gctaaccaca tacatcctaa ttctagaaat agagcatatt tagtatctcc tccacttctt
     1201 aaaataccaa ttataaccaa tgtattaaaa gatttaagag ccattagtat tcccatgata
     1261 aagaatatct tagttatatc agaagctaag ctatcagaaa cactaaacat ttttaataat
     1321 aatggtatat ttaaaattaa aataattcca agtattaaac cagctgaaaa tacaagtata
     1381 gaaaactttt tagcatattc aatggctctt ttaatatggt cagctccaag ttcatttcca
     1441 agcataatag atgcaccaga tgcaatacat acagctgtca ttatgaagaa attacctgta
     1501 ctggttgcta tctggctagc tgctatagca gaagtaccag ctcttgcata tgcaacagaa
     1561 tacaggacag ttccaattgc ccatacagag tcatttacaa atataggaaa gctttttgat
     1621 gaaaaagatt ttataaaaat tttatcaata gcttttagat tttttagacc aaactttaat
     1681 atatagtctt ttttataaag gtaaacataa gtaatcatta aaattaattc acatattcta
     1741 gcaataacag tagctagagc agcacctttt acacccaatg cgggaagacc gaagttacca
     1801 aatataaatc cataatttaa aataacattt gttacaagtg caaatgcact acaaatcata
     1861 cctaacttag gatttctaac acctcttgaa ccagtactaa atacagtact tacagcaatt
     1921 aatggataac taaatactgc aatactaaag taatctatac aaagttttac cacctcagaa
     1981 tcatacgaaa atatgtgtat aattggcttt ggtgttagta aagcaggaat aacaaataca
     2041 aaacttaaaa caattgccaa tacacaagta agacctgtga cttttcttat attactcaca
     2101 tccttctttc cataaaactg tgaaataaaa actccagcac ctcctgtgat accagataag
     2161 gacatattaa aaagaaagaa aaattggttt gcaactccaa ctgaagctac agatgtctcc
     2221 cccaaactac ttatcataat tgtatctatt acatttacag aagtagaaat taagttctgt
     2281 atgataatag gtatacataa agatagaagt attttataaa atcttttatt ttcgtctttt
     2341 aaagaaaata gttcttgcaa ataatcctct cctttttaca taaattcttc ccactcatca
     2401 tatagagagg caaggttatt ttctaaatta attttttctt ggctaacttc tttagcttta
     2461 tctggattag agtatacttc ttcttgacat aaaagtatat ctaatttttc aatttcaatc
     2521 tctaactttt caatttcttt ttctatattt tgtcttttta ttctattttt cttttctatt
     2581 tctctttgtt ctctctcttt tcttttttca tcttttattt gagttctagt tttttcttct
     2641 ttttcttcta taatactcat ctcttggatt tgcctttttt tatctatata ataatcataa
     2701 tttcccagat attcagtaat tccattttca tctaaaacta aaattttatc aacaacagta
     2761 tttagaaagt atctatcatg tgatattgta aatatagtcc catcataatt agttaatgcc
     2821 tcttcaagga cttccttaga atcaatatct aggtgattag taggctcatc taataacaat
     2881 aagtttgcat ttgatagtat aagtttaagt atggcaactc tagctctttc tccaccactc
     2941 aatgtagaaa tcttcttaaa aacttcttca tctacaaata aaaatgcccc taacatattt
     3001 ctcaaagtag tttgagtaag atgagtatta ttttcccaaa tctcatcaat tattgtgtta
     3061 tctaaattaa gagttttttg ttcctgatgg aagtaggaaa catgtacatt agttccaaat
     3121 ttaatattgc cactaagagg ttgtagttca ttagttatta ttttaaataa tgtagatttg
     3181 ccaacaccat ttgcacctat aagtgcaact ttttcccctc tatatatatc taaatccaag
     3241 tctttaaaaa gtattctttc tccataaccc atagatacat ctctaatttg aagaacatca
     3301 ttaccactct ggacagaagg cgtaaattgt atacgagctt ttttcctata agcttctggt
     3361 ttatctaaaa cgtctacctt gtctaatgct ttttctttac ttcttgcacg ctttaggtgt
     3421 ttttctctac cataagcttt tagtctttca attgattctt cttgcttttt tatttctttt
     3481 tgttggtctt caaatttctt aagctctagt tctttttcta ttttagataa ctcaacaaat
     3541 ttagagtagt taccattata tactttaagt tttttgttgt gaacttcaaa aattctattt
     3601 acggattggt ctaagaaata tctatcatga gatattaaca tgacagttcc tttatattgt
     3661 tttaaaaata cttctaacca ttctattgct tcagagtcta agtggttagt aggttcatca
     3721 agtaacaata gagttggttt cttaagaagt aattttccta aaagaactct agttttttca
     3781 ccaccagaaa gtatgcttat tggcttatcc atatcattgt cactaaatcc taatcctttt
     3841 agaactcctt tggcttcaga tttatatcca tatccattta aatctgaaaa taattctagt
     3901 ttatgtgaat attcatccat gattttttct ataattggag agtttgtttt tgtactttct
     3961 tcagatattt taacctccaa ttctcttaaa taggattcca tttctatcaa atttttaaaa
     4021 acttctagca cttcttccaa aatagtatta tccgaataaa aattggtatt ttgttctaaa
     4081 tatccgattt cacaatcttt agaagtataa atatctccac tatcatatcc ataaattcct
     4141 gaaattattt taaataaagt agtttttcca gtaccattaa caccgattat accaatttta
     4201 tcgccttcat ttactgtaaa actaatattt tctaatatag aatctatacc aaaactttta
     4261 tttaagttat tacatgacaa aacaatcata aatttctacc tccttgaact atctaaaatt
     4321 ataatgtttt gatataatat aatcaaggtt atatgtatta gttttgaaaa agaaatattt
     4381 tattgtgaat caaacaaaaa agaggacact tttaggaggt gaagatgttg ggaaataaaa
     4441 atatatcaat ggcagttata agaaggctcc caaaatatca tagatatctt ggagacttat
     4501 tagataggga tatacaaaga atatcttcta aagaattgag tgatataata gggtttaccg
     4561 cttctcaaat aagacaagat ttaaacaact ttggtggatt tggacaacaa ggatatggtt
     4621 ataatgtaga agctcttcat actgagatag gtaaaattct tgggttggat cgaccataca
     4681 acgcagttct tgtaggagca ggtaacttag gacaagctat agccaattat gcaggattta
     4741 gaaaagctgg attcgagata aaagctttat ttgatgcaaa tcctagaatg ataggtttaa
     4801 agataagaga gtttgaagta ttagattcag atactttaga agactttata aaaaacaata
     4861 atatagatat tgctgtatta tgtataccta aaaatggagc acaagaagtt attaatagag
     4921 ttgtaaaagc tggaatcaaa ggtgtatgga attttgcacc tttagattta gaagttccga
     4981 aaggtgttat agttgaaaat gtaaacttaa cagaaagttt atttacctta tcgtatttaa
     5041 tgaaagaagg aaagtagaca acttaaacat attaataatg agcagttgtg aagagtattg
     5101 agaaagtcag aagatttaat attaaatcct ctggcttttt gaaatattag gataaaaatt
     5161 taaataatgt atggaaataa acaatattaa ggagagtggt ttttgtggag gaattatgga
     5221 aaaaggcaat tgaatttcat ggtcatgaat gtccagggtt agctatagga tttaaagcag
     5281 cacttgcagc aagaaaatat ttaggatgta aacagtcagg agatgaggaa attgtatgta
     5341 tttctgaaaa tgatgcttgt ggaatagatg gtatacaagt cgtgctaagt tgcacaatgg
     5401 gtaaaggaaa tttactactt aggatgactg ggaaatcagt atatcatttc tttaataggg
     5461 aaaatggaaa atctataaga atatatgtaa agtcaaagcc atttcaaatg gatagagaag
     5521 gatacaaaaa gtacttatta gaaagtgatt ttgaagaaat atttgagatt acagatacta
     5581 agttaagact tcctgaaaaa gcaagaatat ttaaatctct taaatgtgaa gtgtgtggtg
     5641 aatttgcatc tgagcaatat ataaggattg aagaaggaaa gaaagtatgt cttgattgct
     5701 ttaataaata tgaaagattt tatgaggagt attaaaatta taaattagat taaagttaaa
     5761 agtattagat atgtactaac agtattttaa gatgaattag gtagcaacat aataaaatat
     5821 ataaataata ttacttgaac catacataat taaaaagagg ctgattagta tttagaaaat
     5881 aaaatcagtc tctttatgaa taacgaatta ttattctggt tgaggtgcat ctacaggaca
     5941 agctcctgca caagaaccac agtctataca agaaccagca tctataacat atgcgtcgtc
     6001 tccagctgat atacagctaa cagggcattc agcctcacaa gcaccacagc ttatacaagc
     6061 gtcagtaatt ttatatgcca ttttgaaatc ctccctttta tatgtattgt aattcaataa
     6121 cattataaca aatattattg aatataacaa ttaaattaat tctacaacaa aaactttatt
     6181 atgatattct tgttttaaag ttttacaata aagtttaatc tgcttatatt tttatttatt
     6241 ttaaagaagt ttattttaaa aataaaatta aatttaaaaa gttatgtatt gattatattt
     6301 aaaaatgtta tactctaaaa aagtatattt tattttaaaa tttattaata caaaggagat
     6361 ttactatgaa aaataaatta gtttacatat gtattatgag tctattgcta tttgtagtag
     6421 gatgttctaa taatccaaat aaggaaagta ccattaaaaa agactctaag aataaccaaa
     6481 gccaagttaa agaagaaaaa agagaacctg tgaaaaaaga tataactata agtttcgctg
     6541 gtgatgtaac tatggggaac tataaaggtt catcatatta tggcagtttt gaccatgaat
     6601 ttaaaagaca aggagaagat tacaattatt ttcttaaaaa tgtaaaacct gtatttgaaa
     6661 aagatgactt aacagtggtt aatttagaag gtccacttac tacagcgtca aatgcaaaag
     6721 aaaaaaaatt tgcatttaaa ggaaagcctg gatatgtaaa tattttaaaa tctggaagta
     6781 tagaggcagt aagtattgca aataatcatt cagaagatta ttttgaagaa ggaatgaaag
     6841 atacacagtt tatattggat gagaataaaa taaattattt tggattaggg aaagatgctg
     6901 ttgtagatgt caaaggaatt aaagtgggtc tattagggta taatggatta tcaactgaat
     6961 ataatgaaaa aaatttaaaa caaatggaag atgatattaa gtccttaaaa aagaaatcgg
     7021 atgttgttgt agtgtacttt cattggggga tagaattaga ttattatcca gataaaaaac
     7081 aaaaagattt tgctcattat gctatagaca agggagcaga tttagttatg gggtctcacc
     7141 cacatgttat tcaaggaata gaaaagtata aaaataagta tatagcttat agtctaggga
     7201 atttttgctt tggaggaaat aagaatcctt ctgatacaga tagttatata tatcaacaaa
     7261 cctttacttt ttcagataat aaattaactt caattaaaga gccaaatata ataccaacct
     7321 caataacatc tagtagctcc agaaataatt atcaaccaaa agtattggat ggaagtgaga
     7381 aggatagagt tttaaataaa cttgaaaaaa taagcaaaga tttaaataaa taaaatgtaa
     7441 aaaaaaataa atatatatat ttattttaat aatttatatt ttgtttaggg tatggtaaat
     7501 cgtggtatta aataagtaga ttttaatagt taaattatag tcaattataa taaagctatt
     7561 taatctactt ttttaatata aaaatataag ggggaatttg taatggcttg taacacttgt
     7621 aaaatgtgcg aaagtgcaga caagaaatta gaaagttttg tagcttcaaa ggatgttgaa
     7681 acagcatttc atagaacaga agatcagaaa gttaagtgtg gttttggact tcagggggtt
     7741 tgttgtagac tctgttcaaa tggaccatgt agagtaactc ctaaatcacc aagaggtata
     7801 tgtggagcag atgcagacac tatagtagca agaaactttt taagagcagt agcatcagga
     7861 gcagcttgtt atttacatgt ggtagaaaat acagctaaga atttaaaaaa tgttggaata
     7921 actaaaggtg ttgttaaagg agaaaaaaca ttaaatcaat tagcagagat gtttggaata
     7981 gaaagtgatg aaaagtatga taaatgtata aaggttgcag acaaagttat caacgatttg
     8041 tataaatcaa gagatgataa aatggaatta gtagaaaaaa tagcttatgc acctagagtg
     8101 aaaaagtgga aagagttagg tataatgcct ggtggtgcaa agtcagaagt ttttgatgca
     8161 atagtaaaat catcaacaaa tttaaatagt gatccagtag acatgctagt taattgtttg
     8221 aacttaggta tatcaacagg attatatggt cttacactaa ctaatctttt aaatgatgtt
     8281 atgttgggag aaccagttat aagaatggct cctgtaggat ttaatgtaat agacccagac
     8341 tacataaata taatgataac aggccatcaa cattcaacat ttgcaaattt ccaagataga
     8401 ttaaaagatg aagatgttat aaaattagct caatctgttg gtgcaaaagg ctttaagcta
     8461 gtaggatgta catgtgttgg tcaagattta caattaagag gagaacatta tcaagaagta
     8521 tttgctggac atgcaggtaa caattttaca agtgaggctg ttttatcaac tggagcaata
     8581 gatatagttc tttcagaatt taactgtact ataccagggc ttgaaccaat agcagataaa
     8641 tataaagtaa aaatggtttg tttagatgat gttgctaaaa aagcgaatgc agaatacata
     8701 ggtttagaca gaagtaaatt agatgaactt agtaacactt taatagaaaa agcattggag
     8761 tcttataagg agagaagagg aagtattgag atagatatac caaaagacca tggatttgaa
     8821 caatctttaa ctggtgtaag tgaaaagaac ctgaaagcgt ttttgggaga ttcatggaaa
     8881 cctcttatta atttaattgc agaaggaaaa attaaaggtg ttgcagctgt tgtaggatgt
     8941 tcaaatatga cagcaggagg acatgatgta aatactgttg aacttactaa agaattgatt
     9001 aaaaaagata taatagtact atctgcagga tgttctactg gaggattaga aaatgttggt
     9061 cttatgtcac ctggagcaga agaattagca ggagaaaact taaaagaagt atgtaagact
     9121 ttaggaatac caccagtatt aaactttgga ccatgtctag ctataggaag actagaaata
     9181 gtagcaacag aattagcagc tgaacttgga attgacctac cacaattacc attagtgctt
     9241 tcagctccac aatggttgga agaacaagca ttagctgatg gagcgtttgg tcttgccctt
     9301 ggcttacctc ttcatttagc tttacctcca tttataacag gaggaaaatt ggtgacagaa
     9361 gtattaacag aaaaattaaa agacttaaca ggaggacatg ttatagtgaa tccagaccct
     9421 aaatcttcag ctaatcaatt agaagaaata ataatagata gaagaaaaaa tttagggtta
     9481 aaggatgtgg aatgcaatgc atagaatttt tataaataaa gatttatgta caggatgtaa
     9541 gagctgtgta ctagcatgta tgctaaaaca caataaagat tatgacatgt acactcttga
     9601 tttagaaaat atagataatg atagtagagg gcatatagaa ttagatagta aacataataa
     9661 tccagttcca attctttgta gacactgtga tgaacctgaa tgtgtattag catgtatgag
     9721 tggtgcaatg cataaagata gtgaaagtgg aatcgtttca tatgatgaag agaaatgtgg
     9781 ttcatgttat atgtgtgtaa tgtcttgtcc atatggatta ttaaagccag atgatagaag
     9841 caagcaaaac atacttaaat gtgatttatg taaagatgag gagtatccta gatgtgtagc
     9901 taattgccca agtggagcaa ttgaattaca gaaggaggaa aatgatgaat tatgtagtgt
     9961 taggggctag tgcagctggt ataaatgcag taaaaacatt aagagaatta gataaagatt
    10021 caaatatagt agtaatttct aaagatgaaa acgtatattc aagatgtatg ttgcatcacg
    10081 ttatatctga acacagaact ttaaaacaaa taaattttgt tgatgaagat tttatggagc
    10141 aaaataatgt aaaatggatt gcaggtaaga ctgttaaagg tatagacata aataaaaagg
    10201 tagttcaaac agaagacatt actgtaaatt atgataaact tttaatagct acaggtgcat
    10261 cttctgctat tcctcctata aaaaatttaa gagatggaaa ttttgtatat tctgtgagaa
    10321 acatagatga tatttataaa ataaaagaaa aagcacaaaa ttctaaaaat gtagttataa
    10381 taggtgctgg tttagttggt atagatgcat tagtgggatt atttaaatat gagcagttaa
    10441 atatttctgt agcttttatg gaaaagtaca ttttagatag acaattggat gaatatacag
    10501 catcagtgta tgaaaataag ttcaaagaaa aaggtgtaaa attttaccca agtgcatcaa
    10561 tacaagaaat tgtattggat aacagtaaaa atgttacagg tgttgcattt tcaaatggtg
    10621 aagttttaga tgcagacatg gtaattgtag ctacaggggt taaaccaaat gctgactttt
    10681 tagatgggac aggaatagaa tatgatagag gtataataat agatgatatg tgccaaacaa
    10741 cacaaaaaga catatatgca gcaggagatg ttgtaggtaa aaatgctatt tggcctctgg
    10801 cagtaaagca aggaattgta gctgcctata atatggttgg aaaagataag aagatagaag
    10861 atgaatttgc ttttaaaaat agtatgaatt tcatggatat accaactatt tctataggaa
    10921 tgaatacacc tgtagatgat agctataaag tattaaccag acatggaatt aatgattata
    10981 agaagtttgt ttttaaagat aatgtaatct acggagcagt tatacaagga gatatttcat
    11041 atgtaggtgt attaacttat ttaataaaaa ataaagttga aatatatgat ttagaaaata
    11101 ggatatttga tgtatgttat gcagactttt ttaatataaa agaaaatgga gaattttgtt
    11161 atagtgtata gtatatacgt ggagtaaggt ttataaatac agtaaatcaa taaatagagt
    11221 tattctaaaa taagaaaaat gacaaatctt atttagaaat agctctattt tttgtaatta
    11281 aatttaaatt atattcaatt tgtaaaatat tattttgaac aattatacat atactctcta
    11341 gttagaggta aatcattttt tataccttta gtgaaaagta tttgatgtat atctataata
    11401 ccagtgttga aagcagcagc acaagaacaa aggtataaat cccacattct tataaactta
    11461 tcatcaaaca tttctttaat ctttggaaga ttctcagaga aacttttttg ccagtatagt
    11521 aaggttttta cataatgtaa tcttaaactt tctacgtcta tagtatgata tttgtaatca
    11581 ctacaaatat gtatcatttc tcttaggcta ggaataactc ctcctgggaa aatatatttt
    11641 tttatccaag ggtctccata agattcctgc aatccactta tatagtgaag taaaaacaat
    11701 ccaccatctt ttaaaacagc atttacgttt ttaatgaaaa gttcatagtt ttctctacca
    11761 acatgttcaa gcattccaac gcttacaact ctatcaaaca taaggtctga tttttttaat
    11821 tctctatagt ccataagtct tacctcaaga tagtcttgaa ggttctcttc tttaattctt
    11881 gcgttaaatt ccttaaattg ctcttcgcta agtgtaattc caacgccgtg aattttatat
    11941 ttttttgcag cttctattag taaaaatccc caaccacaac caatatcgag aaggctcata
    12001 ccttcctcta aatttagttt agttaatatg tggtgaactt tattgacttg agcatcataa
    12061 agagtatcat tatcatttct aaagtatgca caagaataac taagtgtctt atctaaccat
    12121 aagctataaa aatcgttgcc aatgtcatag tgggcatgta tttctttctt ctgatgtctc
    12181 attgaatttg atgagtgcaa tagatgtttt acagctgaat tatcaatatt gaatttgtct
    12241 atttgtttta aaagtagatt taaagttaag aataaatctc cctcaacttc aatgtctcta
    12301 tttatgtagg cttctccaag tgctaaagat gtacttttta atagttcaga cttgctaata
    12361 tcattattta ctataacttt gaactgaggg gagccctctc caattagaaa ttcttcctca
    12421 tctttaagcc taactagaaa tggtgtttca ccaaatttct ctaaatagtc ttttaaaaaa
    12481 ttttttgtaa tattcatatt accagctcct tctaatgtaa atccagtaag tttccctgct
    12541 taaa
//
LOCUS       DAGQWR010000121        12435 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.121, whole genome shotgun sequence.
ACCESSION   DAGQWR010000121 DAGQWR010000000
VERSION     DAGQWR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.121"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(78..635)
                     /locus_tag="MIW95_002964"
     CDS             complement(78..635)
                     /locus_tag="MIW95_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBY3152275.1"
                     /translation="MRTLKYAILGLLNRNPMTGYDIVKEFNFQLAEFWNAKHSQIYPE
                     LKKLVNEKLIVYDIKISGDVLEKKLYTITEKGKDEFLKWLEKDEPMERTPKDIFRLRM
                     YFSNNLDIDTRLILLEHQLLQHQERITFLYSQMERYSEVPPLDSNDFGDYIVLDGAII
                     REQGAIKWLTNCINHHKNLDNTTLK"
     gene            887..2185
                     /locus_tag="MIW95_002965"
     CDS             887..2185
                     /locus_tag="MIW95_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HBY3152276.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEHLNSLAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLIFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVANW
                     IINKYGVTSAFKSIGLVFLIIVCVSSFFIKKCPADFTPEGWVPPEKKGKVRVVINKNW
                     KEMLSTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDSAIVVSILALFNTL
                     GRIVAGYISDKIGRINTLSGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYIGPAVMKNVYSVDGNYQRAFIIAIII
                     ALIGFILTFLYKYINKIQNLKIENLIKGDIKNGKKISKSM"
     gene            2157..4106
                     /locus_tag="MIW95_002966"
     CDS             2157..4106
                     /locus_tag="MIW95_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBY3152277.1"
                     /translation="MERKYPNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMIHGGHGWLINQFLSPYFNKRTDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGTEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPVATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVVSVLKPKKVL
                     IAGGGPGGLQAAITAVKRGHKVILCEKTNELGGILKGEQALPFKYKMYELGNTFGKIA
                     KDLGVEVRLNTTVTKEYVENENVDALIIAVGSEPLVPPIEGLDGENVVIVNDYYVEKE
                     KVTDEVIVLGGGLAGCEAAIHLAQEEKTVHLVEMRAELAPDANIRHRPILLEEIERQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDTLLDSAPYVA
                     QIGDCVRASTITNAVYQGHHAALDI"
     gene            4543..4680
                     /locus_tag="MIW95_002967"
     CDS             4543..4680
                     /locus_tag="MIW95_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3152278.1"
                     /translation="MINENLKSLRKIHQYTQEELAEKLNVSRQSIAKWESGVSQTKGY
                     Y"
     gene            4908..5282
                     /locus_tag="MIW95_002968"
     CDS             4908..5282
                     /locus_tag="MIW95_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBY3152279.1"
                     /translation="MFNLIEMLFPIMFILIFIIIVFTFVKGIATWFKNNNSPRLTVSA
                     RIVAKRQNTTHNNQPNAGDISGAHGFHTISSTSYYVTFQVDSGDRIELSVSGSEYGVL
                     AEGDKGQLTFQGTRYLTFDREN"
     gene            5298..5945
                     /locus_tag="MIW95_002969"
     CDS             5298..5945
                     /locus_tag="MIW95_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152280.1"
                     /translation="MNKFLSQIRRIDNEISITRKIINTIFILCFGIVLGTFAKFLDTT
                     ASNTLPFIFEYLDISNFFGRFAIWLLIALYIAIYSHSSIRASLNVLVFFIGMVSSYYL
                     YSYFVAGFFPKNYAMIWLGFTVISPLLAFICWYAKGKSKISFILSVIIIAILFNFTFI
                     YGWIYFDVYSILEVIVFGCALIALKRNTFRETTYMILSAVVIAVILNMLVPFHFG"
     gene            6325..7404
                     /locus_tag="MIW95_002970"
     CDS             6325..7404
                     /locus_tag="MIW95_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBY3152281.1"
                     /translation="MNDMKFIETLKQKRNVCDYSQSRLAQELQINRQNLNEIENGKTK
                     ASKEMKHILLHYLDYCNCTQPFTLTIDYLRVRFPTTDALEIIKNVLAMKSEYFIHEDY
                     GMYAYEEQYIYGDISVNASKDSSMGVLLELRGMGCRDLEYVLQVRGIDWYCFLRCCID
                     YQGVFKRIDLAVNDMGGLLDIEILRERYYANKVWKRSRTHEAVDSGKLSGTHGDTAKT
                     FYIGSKNSSIYFCLYEKEKEQKSKGIKTDIKNRFEIRLKNGKAGQTVEQLVFSRNPEQ
                     TIASFILTQIDFPDYILWDIFLDNVTTSLPFIMTPVAVNMDKIKRWLERQVMPSLLMI
                     KEIEKKTGANYLEEIDRHTRLTEKQ"
     gene            8226..8439
                     /locus_tag="MIW95_002971"
                     /pseudo
     CDS             8226..8439
                     /locus_tag="MIW95_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
     gene            8551..10164
                     /locus_tag="MIW95_002972"
     CDS             8551..10164
                     /locus_tag="MIW95_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY3152282.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIVEKIFSLAYQGYGSAKITKVLREEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNTANKTPYSYYACSYYGQFGKGTCSMHYIRYDVLYQAVLKRLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASAFKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITNLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEAITNEDNERIQEIEIYYRFIGKVD"
     gene            10193..10516
                     /locus_tag="MIW95_002973"
                     /pseudo
     CDS             10193..10516
                     /locus_tag="MIW95_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            10548..11735
                     /locus_tag="MIW95_002974"
     CDS             10548..11735
                     /locus_tag="MIW95_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBY3152283.1"
                     /translation="MSKIVFFSILAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIK
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFITHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            11927..12331
                     /locus_tag="MIW95_002975"
     CDS             11927..12331
                     /locus_tag="MIW95_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBY3152284.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYHALYVNKVMLQI
                     YDVENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT         4532 a         1616 c         2393 g         3894 t
ORIGIN      
        1 caaagacaaa gttgtgtaca gtctttttta taaatttaat gtctataatt aaatacacta
       61 ttaaaatcaa taagttatta tttcaaagta gtattatcca aatttttatg atggttaata
      121 caatttgtta gccattttat agcaccttgt tccctgataa tagctccatc aagaactata
      181 taatcaccaa aatcattact atcaagagga ggaacttcac tataacgctc catttgactg
      241 tacaagaacg taattctttc ttggtgttga agcaattgat gctctagtaa aataagccta
      301 gtatctatat ctaaattatt agaaaaatac attctaagac gaaaaatatc cttaggggtt
      361 ctttccattg gctcatcctt ttcaagccat ttcaagaact catctttacc tttttctgtg
      421 attgtgtata actttttctc taatacatca ccactaattt taatatcata tacaataagc
      481 ttttcattta ccaatttttt tagttcagga tatatttgac tatgctttgc attccaaaac
      541 tcagctaatt gaaaattgaa ctcctttacg atatcataac cagtcatagg atttctattt
      601 aataatccta aaattgcata ctttaaagtt cgcataaata cacctctttc tttaattaca
      661 tacttattat aacacgatat atttagtttg aattaattaa tatagctatg ttttaataag
      721 aacttttaaa ttatgaggaa caaaaaataa ataaaactat atataaaaat gaaattatgt
      781 aaatataaac atgtaagtat ttacatgttt atatttacat atagaaaaaa ttgtgataat
      841 cttaactaag aaattctatt taataaattt atcgaggagg agagtaatga atttaacaca
      901 aaaaagatgg attatattaa ttgcaagttg tttcatcaat ttatgtattg gttcaatata
      961 tgcgtggagt gtatttgcat cacctatggc agaacattta aatagtcttg ctggaacaac
     1021 ttttacagct gctagtttag caattgtatt tacagttaca aactcagttg gacctattac
     1081 tatgattaca gggggaatga taaatgataa atttggtcct aaaaaagttg tatttgtagg
     1141 aggattaatt tttggtatag gtatgatatt ggcaggtttc tcaaaaggat tgcctatgtt
     1201 gattttatca tatgggataa taacaggtct tggggttggc atggtttatg gatgtacaat
     1261 aagtaattca attaaatttt ttccagataa aagtggatta gttggtggta taacaacagc
     1321 agcttatggg tttagttcag taataatgcc tccagttgct aattggataa ttaataaata
     1381 tggagtaact tctgcattta aatcaatagg tttagtattt cttataattg tatgtgtttc
     1441 ttctttcttt atcaaaaaat gtccagcaga tttcacacca gaaggatggg taccaccaga
     1501 gaaaaaagga aaagtaagag ttgttattaa taaaaattgg aaagaaatgc tttcaacacc
     1561 tatattttat ctaatgatta taatattaac ttgtggagct ttctatggat taatgtgtgt
     1621 gtcattagca tcttctctag caacaaatat gattgggatg acaagttcag attcagcaat
     1681 agtggtttca attttagcct tgtttaatac attaggccgt attgttgcag gatatatatc
     1741 agataaaatt ggtagaataa atactttatc aggtgcatta gttatttcaa tttttggttt
     1801 attatgtttg ttattctcaa gagaaggaga tgtagcaaca ttctatattg gaataataat
     1861 tataggtata tgttttggtt catttatggg aattttccca ggatttacag tagataaatt
     1921 tggacctaaa aataatagtg taaattatgg aatcatgttt attggttttg cttttgcagg
     1981 atatattgga cctgcagtga tgaaaaatgt atattctgta gatggcaatt atcaaagggc
     2041 tttcattata gctataatta tagctttaat aggttttata ttaacatttt tatataaata
     2101 tataaataaa atacaaaatt taaagataga aaatttaata aagggagata taaaaaatgg
     2161 aaagaaaata tccaaatcta tgtaagccaa ttaaaattgg aaatgtaaca ttcaaaaaca
     2221 gaatgttttc agcacctatg gggggaacag acataacagc agattgtaca attggctcaa
     2281 aatcaacagc cttttacgaa ttaagagcaa aaggtggagc aggtgcagtt acaattagtg
     2341 aatgtatggt tcatccagaa acagatggtt cacatgcata tcatctagac ttaaaaatag
     2401 ttgattcctt agcaagtttt acttatacag cagatgcaat tcgccgtcat ggagcaatac
     2461 caagtgtaga actatctcat tcaggacaat attcaggaac ttatttagtt gacaaagata
     2521 agaagcgagg attatcacaa tggggagtaa gtcctagtgt tcgtcctgat ggattagaga
     2581 taaaagaact tacagaagaa ataattgctg aaatagtaga gtcatatgga aatgttgcag
     2641 cattggctaa gagagcaggg tttgagatga ttatgattca tggtggacat ggctggctaa
     2701 tcaatcaatt cttatcacct tactttaata aaagaactga caaatatggt ggttctttag
     2761 aaaacagagt tcgttttgct aaagaagttt tagatagtgt tcgtaaagca gtaggtccag
     2821 gatttccaat agagtttagg atgagtggtt ctgaattatt tgagggaggg tatgatttag
     2881 attatggaac agaaattgct aaattattgg aatcaagagt agatttactt catgtatcag
     2941 caggtacata ccaaagaggg tttgcagtaa cacatccttc tatgttctta gaacatggat
     3001 gtaacgtata tcttgcagaa gaaattaaaa aacatgtaaa tattccagtt gctactatag
     3061 gagcattaaa tgacccagaa atgatggaag agataattgc tagtggaaaa gcagatgtta
     3121 tttacatggc tcgtgcatta ctagctgacc acgaattacc aagaaaagtt atgagtaatc
     3181 aagatgaaaa aatagtgaaa tgcctaagat gcttcacttg tatggcagaa cgtgctacca
     3241 cttctacaag acgctgcact gttaatccac ttattggacg tgaattagat ggaactgaag
     3301 ttgtttcagt attaaaacct aaaaaagtat taatagcagg tggtggacca ggaggattgc
     3361 aagcagccat tacagctgta aagcgtggac ataaagtaat actttgtgaa aaaactaatg
     3421 aattaggtgg aattttaaag ggagaacaag cacttccatt taagtataaa atgtatgaat
     3481 taggaaatac ttttggaaaa atagcaaaag atttaggtgt tgaagtacgt ttaaatacaa
     3541 cagttacaaa agaatatgtt gaaaatgaaa atgtcgatgc attaattata gcagtagggt
     3601 ctgaacctct tgttccacca atagagggtc ttgatggaga aaatgtagta atagttaatg
     3661 attattacgt agaaaaagaa aaagtcactg acgaagtaat agttttaggt ggaggattag
     3721 caggatgtga ggctgcaatt cacttagctc aagaagagaa gactgttcac ttagtagaga
     3781 tgagagcaga acttgcccca gatgcaaata taaggcatcg tccaatatta ttagaagaaa
     3841 tagaaagaca aggtatatat gtatatacag aacataaggg tttaagtgta acaacagaag
     3901 gtgtagtatg tatgaataaa tctgggaata aaataaatat tccaggaacc tcagtaatat
     3961 gtgcacttgg acaaaaacca agaagagaag tggtagacac tttacttgat tctgctccat
     4021 atgttgctca aattggagac tgtgttcgtg catctacaat aacaaatgca gtataccaag
     4081 ggcatcatgc tgcgctagat atatagttaa aaagacgtag atagtattgt tttattataa
     4141 aaaaggagtg tctcaaaatg aactttttag ttcatgagac acttttttac atgaaattaa
     4201 atatattttt atcatttcaa caatgaaaaa agattatctt tattttgaga cagcactttt
     4261 gttattatga ttttaattct tattgcaagt tttcatttga ttgatgagta aaacttttta
     4321 agatgtagaa gtatttaaca atgtaaatga ccctaactta aatactttag cgaaggttga
     4381 cagatttgtg tttgctttta tctattataa cttaaggaaa catgttacct gaacaataaa
     4441 tttatataat aaaagtatat aaatatatat atgaatgtta agattagttg atagaattta
     4501 aataataaat ccaatattat aaacatagga ggtatgaaaa atatgattaa tgagaattta
     4561 aaaagtctaa gaaaaatcca ccagtataca caagaagaat tagctgaaaa acttaatgtt
     4621 tctagacaat caatagcaaa atgggaaagt ggagtttccc aaacaaaagg atattactga
     4681 aataacctgt gtttacctgc ttgtagagaa aagaatattg ctattaagcc agagatacta
     4741 cataaatgtg ttggtatctc tttttcgtta atgcaacaca gtaaattatt tcattttcct
     4801 aattatttta gactgataag taacttttta aatgaagaaa agaaatgaca atacttaaag
     4861 gagcatggta tacttaaagg agaatttcat taaggaggaa aaagagattg tttaatctaa
     4921 tagaaatgtt atttcctata atgtttatac tgatttttat aataattgtt ttcacttttg
     4981 tgaaaggaat agctacttgg tttaaaaaca ataattctcc tcgcttaact gtgtcagcaa
     5041 gaatagtagc aaaacgtcag aataccactc ataacaatca accaaacgct ggtgatatat
     5101 caggagcaca tggatttcat acaatttcca gcacctcgta ttatgtgaca tttcaagtag
     5161 atagtggtga tagaatagag ctgtctgtat ctggctcaga gtatggagtg cttgcagagg
     5221 gagataaagg acagctgaca ttccaaggca caagatattt aacatttgat agggaaaatt
     5281 gaatttagag gtgcattatg aataagtttt tgagtcagat aaggcgtata gataatgaaa
     5341 tatctattac taggaaaatt ataaatacaa tatttatact gtgttttgga atagttttag
     5401 gaacttttgc aaaattttta gatactacag caagcaatac tttaccattt atatttgaat
     5461 atttagatat tagtaatttt tttggtcgtt ttgctatatg gttattgatt gctttgtaca
     5521 ttgcaattta tagtcattct tccataagag catcattaaa tgtgttagta tttttcattg
     5581 gaatggtttc aagttattac ctatattcgt attttgttgc tggatttttt ccaaaaaatt
     5641 atgcaatgat ttggcttggt tttactgtca tttctccatt gttagctttt atttgttggt
     5701 atgctaaggg gaaaagcaaa atatctttta tactttcagt aataataatt gcaattttat
     5761 ttaattttac atttatttat ggttggattt attttgatgt gtattcaatt ttagaagtga
     5821 tagtctttgg ttgtgcatta atagctttaa aacgtaacac ctttagggag acaacgtata
     5881 tgatattgag tgcagttgtt attgctgtta tacttaatat gttagttcca tttcattttg
     5941 gttaggcaga ttttaagatt attatagata gaatataaat ttcacgaacc gccgaagcga
     6001 tccgccagcg gtggcggtcg taagacatat cgccacgcca gcttgctggc gaggtggcgg
     6061 ttcactttgg cggtttggcg agcctgcgag ctttctaagc ccccgttatc taacaggggg
     6121 ggactattac aaaagacaag agcaaaaaca aatataaaac tgctgatatg caacgattta
     6181 taaggttgta agaacttatt tcactttaca ccctattctt tctacccgac aggtctaaaa
     6241 cagcatatca atagaaaata aatataagat acaaggtgta tgctcctatg agtatatgcc
     6301 tttttttgat tggaggaatg aagcatgaat gatatgaaat ttattgaaac attaaagcaa
     6361 aagagaaatg tctgtgatta ttcgcaatca cgactggcac aggaactaca aatcaacagg
     6421 cagaacttaa atgaaattga aaacggaaaa acaaaagcca gtaaagaaat gaaacatata
     6481 ctcttacatt atcttgatta ttgtaattgt acacagcctt tcactttaac gattgactat
     6541 ctgcgtgttc gttttcctac cacagacgca ttggaaatta taaaaaatgt gctggcaatg
     6601 aaaagcgaat attttataca tgaagattac ggaatgtatg cttatgaaga acagtatatc
     6661 tatggggata ttagtgtaaa tgcttctaaa gatagttcta tgggtgtttt attggaatta
     6721 agaggtatgg ggtgtcggga cttagaatat gttttacagg taagagggat agactggtat
     6781 tgctttttaa gatgttgtat agattatcaa ggtgttttta aacgcataga tttagcagtc
     6841 aatgatatgg gcggattgct ggatatagaa attctaaggg aacgttatta tgctaacaag
     6901 gtatggaaac gttcaagaac ccatgaagca gtagacagcg gaaaattatc gggtacacat
     6961 ggagatactg caaaaacttt ttatatcggt tcaaagaata gttctatcta cttttgcttg
     7021 tatgaaaaag aaaaggaaca aaaaagcaag ggtataaaaa cagatattaa gaaccgcttc
     7081 gaaattcgtt taaaaaatgg aaaagcagga caaacggtag agcaacttgt tttttcaaga
     7141 aatcccgaac agaccatagc aagctttatt ctcacacaga tagattttcc cgattatatt
     7201 ttatgggata tatttttaga taatgtaacg acctcacttc cgtttattat gacacctgtt
     7261 gctgttaata tggataaaat aaaacgctgg ctagaaagac aggtcatgcc ctctttattg
     7321 atgataaaag agattgaaaa gaaaacagga gcaaattatc tggaagaaat cgacaggcat
     7381 acaaggctaa cagaaaagca gtaattaaag attaaacaaa tgacaacaga catagcagat
     7441 atgattgaga aagataccgc tgttccacaa gggaatgacg gtatttttta atttcttcaa
     7501 aaaaactgca acaaaatgac tgcttgtgta cagatatggt gcgaggaaag gaaatctaac
     7561 tttttttgaa aacaggtcat taaatcggtg gctgctgtcc ctatatgatg tgaaagaaga
     7621 aacgattttt aatttcaact taacatttga cagttttcat tccctatatg gagcaaagaa
     7681 aatcttttat tccatagttg gcagttacgg tgttgcatgg gtagttaggg tgtgaaaaga
     7741 aaaacagatt tttttcatca ccaacttagc aaactgatac ttgctttccc tatatggtgc
     7801 aggaaaagag agtttaacta ttttcatttc aacttagcaa ctggacgttt gctgtacaga
     7861 tatgtagtga aaagagaaaa agttttgaat attggttacg cttcggctct tttccaacgc
     7921 ggtatatagg aaagacgatt ttcttctttc atgttctttg acaactgaat aaagcaattc
     7981 aatacgtttg acagacagcg atccattgaa gcagatacgc catgaccttt tccctgcaaa
     8041 agcgagcgaa ccctatttgt gttcctgtaa aagacttgtc ctcttttatc gccatgacct
     8101 gttctgtctg ataaatgata ctcccgtaca gccacagctt gaacgttcag agcgtcgcac
     8161 gcaatgggta cggctacata agaactatgc agaggtggaa ttcctgtggg ctatgacaaa
     8221 tgccgttgaa ttgcttagaa aaaagattat ggaaaggggg tatgtgttat tgctgatatt
     8281 gtaaaaacaa tgcaagaaaa gacaaataat aagcgtagca ttggcaacag aggaaaaaca
     8341 aagattgttg taaaagagca tttttccgaa aagggaaaga caatggagga acttctgact
     8401 gatgttatgc tggaaaaagc aaagcagaca atcgcataaa attggtgcag ttgtaagaca
     8461 tagattgaat ggcaaaaagt acccgtgtta tactttactt gcaagcaggt attgtaaaca
     8521 cgggttagtt ataaaggagg ataactgaca atgaaacaac agatttacaa tactgcactt
     8581 tatcttaggt taagccgaga tgatgaatta cagggcgaaa gttccagcat taccacacaa
     8641 agaagtatgt tgcgtctata tgcaaaagaa catcatttga atgtgattga tgaatatatt
     8701 gatgacggct ggtcgggaac aaattttgaa agaccgagtt ttcaaagaat gattgaggat
     8761 atagaggcag gaaaaatcaa ctgtgttgta acgaaagacc tttcccgact tggcaggaac
     8821 tatattatga caggacaata tacagaattg tattttccca gccataatgt ccgttacata
     8881 gcgattgacg acggtgtaga cagcgagaaa ggcgaaagtg agattgcacc atttaagaac
     8941 atcatcaatg aatgggtggc aagagataca agccgtaaag tgaaatcggc atttaagacg
     9001 aaatttgcag agggtgctca ttatggtgct tatgctccat taggatataa gaaacaccct
     9061 gacatcaaag gaaaactgct ggttgatgat gaaacaaaat ggattgttga aaaaatcttt
     9121 tccctagcct atcaaggtta tggtagtgcc aaaatcacaa aggtactgcg agaggaaaaa
     9181 gttccgacag cgtcttggct gaattttaca aggtacggta cttttgcaca tatctttgag
     9241 ggaaagcccg aaagcaaacg ttatgagtgg acgattgctc atgtcaaggc aatcttaaaa
     9301 agtgaggttt atataggcaa cagcgttcac aatatgcagt ctacggtttc attcaagagc
     9361 aagaagaaag tgcgtaagcc cgaaagtgaa tggtttcgag tggaaaatac ccatgaaccg
     9421 attattgaca aagaagtgtt ctatcgtgtg caggaacaga taaaatcaag acgtagacag
     9481 acaaaggaaa aagcaacacc gatatttgca gggcttgtca agtgtgcgga ttgtggctgg
     9541 tctatgcggt ttgcaacgaa tacggcaaat aaaacgcctt acagttatta tgcttgcagt
     9601 tactacgggc agtttggcaa aggtacttgt tctatgcact atatccgtta tgatgtgttg
     9661 tatcaagccg tattgaaacg attgcagtat tgggctaagg cagtacagca ggacgaagaa
     9721 aaggtgctga acaagataca gaaagcaggc aatgcagagc gaatacggga aaagaagaaa
     9781 aaggcaagtg cctttaagaa agccgagaac cgacaaaatg aaattgaccg tttgtttgcg
     9841 aaaatgtatg aagatagagc ctgtgagaag ataacagagc gaaattttgt gatgttgtct
     9901 agcaagtacc aaaaagaaca aatagaactg gaacagcaga taaccaacct aagagaagaa
     9961 ttaagtaaaa tggaacagga tatgataggt gctgaaaagt ggattgagtt aatcaaggag
    10021 tattccgtac caaaggaact gacagcaccg ttattaaatg caatgataga aaaaatcctt
    10081 attcatgaag caataacgaa tgaggataac gaaagaatac aggaaataga gatatattac
    10141 cgatttattg gaaaagtaga ctgataaaga gggttcatat ctttaactaa gggaaactgg
    10201 agaatctatt cctgatatag gtagttgcat taaacttgca aaattgtaca aggtcaaact
    10261 agatgactta gttaatcatt cggaagaaaa aacaggtatt atagtacctc ctaaaggaaa
    10321 gtttttcttt ggagcagtag tagttggtga acgtggtcag attgtcattc caaaagaagc
    10381 aagagaagta tttaatataa atgcaggaga taaattattg gtacttggag atgaggaaag
    10441 aggattagga attgttcatc aacgtgattt gataaatttt ataggtcata tgggagtagt
    10501 acgtaaagaa gaatgatttt aaaaaattat aaaaggaggt atgaaatgtg agtaaaatag
    10561 tattttttag cattcttgca tatggtcata ctaatcctac tattgaagta gttcgtgaat
    10621 tagtggatag aggaaatgag gtattgtatt attcttttaa tgaatttaaa gataaaattg
    10681 aaggtgctgg agcaaaattt atttgttgtg ataaatacct tccagaatta ctccctggag
    10741 atgagaaaaa aattggtaag gattttcctg gattaattga gatgatagta gatactacta
    10801 tttctttaga tgaaaaagtt tgtagggaac ttaaagattt taatccagat tgcattgtat
    10861 cagattcatt aagtttttgg ggaaagttat ttgcacaaaa acttaatatt ccatatgtat
    10921 gctctacaac aacttttgca tttaataaac atacagcaaa gttgatgaaa caaaacttta
    10981 tagaaattat acgtatgatt tttggtgtaa gacgcattaa taaaaaaata aagcttttac
    11041 aaagaaaagg gtatgaagta aaaaatttta tttctatagt ttctaatgat aatgatacgg
    11101 ataccattgt atatacatca aaagaatttc aaccaatggt agaaaccttc tctagtaagt
    11161 attcatttgt aggtccatca gtatcaaagt tgattattaa acctaaagag agaaaaagaa
    11221 agttaatata tatttcgctt ggtacaatta ataataaaaa tgttagcttt tataaaaact
    11281 gtataaatgc atttaaagat tctgatgttg atgtgattat gtctgttggt agaagtactg
    11341 atattaaaag tttaggaaat ataccaaata attttgaggt taaaaatagt gttgaacaga
    11401 ttgctatact tcaaaaaact gatgtgttta ttactcattc tgggatgaat agtgttaatg
    11461 aaagtttata ttatggagta cccatggtat tatttcctca acattcggag cagagaatgg
    11521 tagctaagag agttgttcat ttgggagcag gaattatgtt aaaagaggat aagtccgaaa
    11581 gtattaaaaa agcagtattt caagttataa atgacaatga atataaagaa aatgcaacta
    11641 aactatcaaa aagcttttat aatgcaggag gctcaaaaaa agcagcagat gtaatattac
    11701 agattatatg taatagtaga caagttgaca tataatagat ttgttaaaaa tatatataca
    11761 tacatgcaat ttaatcaaag tactgacagt ggggtctttt aacctaccag ttggggcata
    11821 tatattgatg ctaaatatga gggatgttat attttaataa aaaagataac atcctttttt
    11881 tagtgcgtta tatcaagttt taaataataa atgaaggagg attaatatga aaaaatataa
    11941 agttattttt atagtattaa ttataggtat tataagtgta acagggtttg ttgtatataa
    12001 aaactgcatt aaaccatgga aagatgctga aacatatatt gataagtata tggtaaaaca
    12061 agatatttca aaagataata taaaaagtat tacaaaagaa aaagccaaga aggcaagtta
    12121 cgaaggaata ctatacaaag tgtattacaa agatgaccct cgatatgagt atcaatattt
    12181 ttatagtgat aattatcatg ctctatatgt aaataaagta atgttacaaa tttatgatgt
    12241 ggaaaataat aataaacttt taagaagtaa tgaggaatta aaaagtgtaa agtatccacc
    12301 aatatatctt aaatataagc cagataaatg atttatgaca aaatagaatg atttttatta
    12361 aaatttattt aaaaatatca ctagagtgtt taaataaaac taaatttaag tatttatata
    12421 ttagtatatc ttact
//
LOCUS       DAGQWR010000122        12337 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.122, whole genome shotgun sequence.
ACCESSION   DAGQWR010000122 DAGQWR010000000
VERSION     DAGQWR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.122"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            64..267
                     /locus_tag="MIW95_002976"
     CDS             64..267
                     /locus_tag="MIW95_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3152285.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            431..1585
                     /locus_tag="MIW95_002977"
     CDS             431..1585
                     /locus_tag="MIW95_002977"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBY3152286.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELVNITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFRVEQENIDVVVHPQSIIHSMVQYADSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1598..2602
                     /gene="rseP"
                     /locus_tag="MIW95_002978"
     CDS             1598..2602
                     /gene="rseP"
                     /locus_tag="MIW95_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBY3152287.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSNKVVNVTPKNNNGKYEIGIQPQREKDFLASIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2785..3840
                     /gene="ispG"
                     /locus_tag="MIW95_002979"
                     /gene_synonym="gcpE"
     CDS             2785..3840
                     /gene="ispG"
                     /locus_tag="MIW95_002979"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBY3152288.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSRERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKHGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            4188..5012
                     /locus_tag="MIW95_002980"
     CDS             4188..5012
                     /locus_tag="MIW95_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152289.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLASTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSIWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(5485..6608)
                     /locus_tag="MIW95_002981"
                     /pseudo
     CDS             complement(5485..6608)
                     /locus_tag="MIW95_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
     gene            6805..7029
                     /locus_tag="MIW95_002982"
     CDS             6805..7029
                     /locus_tag="MIW95_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152290.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            7082..7606
                     /locus_tag="MIW95_002983"
     CDS             7082..7606
                     /locus_tag="MIW95_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3152291.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(7711..7857)
                     /locus_tag="MIW95_002984"
     CDS             complement(7711..7857)
                     /locus_tag="MIW95_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152292.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(7996..8922)
                     /locus_tag="MIW95_002985"
     CDS             complement(7996..8922)
                     /locus_tag="MIW95_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBY3152293.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9113..10984
                     /locus_tag="MIW95_002986"
     CDS             9113..10984
                     /locus_tag="MIW95_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBY3152294.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNASCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(11076..12011)
                     /locus_tag="MIW95_002987"
     CDS             complement(11076..12011)
                     /locus_tag="MIW95_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBY3152295.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
BASE COUNT         4864 a         1297 c         2086 g         4090 t
ORIGIN      
        1 aaatagatga aaattttcca aaatagataa aatgtgatag aatatatttt gcgaggtgat
       61 aacatgattt catacgctcc attatggaaa ttgttaattg acagaaaaat aaagaaaatg
      121 gaatttgtaa atatatcagg tataagtatt tctgttttag gcagactagg aaatgataaa
      181 tctgtttcta tggatacaat ggagaagata tgtttagcac tagactgtaa aatagaagat
      241 gttgtggaaa taaaaaaggg catttaataa tactagaata ttttataata ttccacaaga
      301 tacaaaaaat aatagattaa ttttaatctg tggaataata tatgaaattt ctatgaatag
      361 taaaaataat tgttttagta gtgttataat gttttgacag ggtatattta ttattagaaa
      421 ggattattat atgaaaaaga tatcaatttt aggttctaca ggctctatag gtaaacaaac
      481 tttggatgtg gttagagaac atagagataa gtttgagata gttgcaatat cagctaacag
      541 taatattgaa ttattgctag aacaaatagt agagtttaaa cctaaatatg taacagtttt
      601 tgaggaaaat aaggcattga aattaaaaga aatgttgcct aaaaatatag aaatagaagt
      661 tttagcagga atggaaggat taaaaataat atcatcactt gatgaagtgg atgtcctttt
      721 aactgctgta gtaggtatga taggattagt cccaactctt tgtgctataa agaagggaat
      781 agacatagca ttagcgaata aagaaacgtt ggtaactgct ggtgaacttg taatgaaaga
      841 agcagaaaaa tacaatgtaa atattcttcc tgttgatagt gaacatagtg ctatatttca
      901 atgtctaaat ggagaaaata aaaaaaatat agaaaagata atacttacag catctggtgg
      961 tccatttaga ggaaagaaaa agggagaact tgtaaatata actaaaaatg aagctttgaa
     1021 gcatccaaat tggagtatgg gaagaaaaat aagtattgat tcttcaacgc ttatgaataa
     1081 aggacttgaa gtgatagaag ctagatggtt gtttagggta gaacaagaaa atatagatgt
     1141 agtagttcac ccacaaagca taattcattc aatggtgcag tatgcagata gctcaataat
     1201 tgctcaatta gggtgtccag atatgagact accaatacag tatgctctta catatccaga
     1261 tagaatggaa agtagctttg aaagaatgaa tttttcaaaa tttagtactt taacttttga
     1321 agaaccagat ttagaaactt ttccatgttt aaaattggcc tatgagtgtt taaaaatggg
     1381 aggaacttat tcttctgttt taaattcagc aaatgaagtt ttagtaagtg aatttttaga
     1441 ggataaaatt ggtttttatg acataccata ttacatagag aagactttag aagttcatag
     1501 tagcataagc gaaccaacat tagaagaaat cttagaaaca gatagatgga gtagagctta
     1561 tgttgcaaat ctgataaaaa aataggaagg tgaatttatg actataatag ccgcattaat
     1621 actatttagt ataattgtat taatacatga gttaggacat tttatatttg caaaaagaag
     1681 tggtatcaaa gtaaatgagt tttctatagg tatggggcct aagatatata gtgtaaaaaa
     1741 agatacagag tattctataa gagcacttcc aattggtgga tatgtcagca tggaaggtga
     1801 agatgaagaa caaataagtc caaattcttt tggaaacaag tctatattac aaagatttag
     1861 tactattgta gcaggaccaa tattcaatat aatattagca gcaatacttt tagttccagt
     1921 atttttgtat ataggttctc caacgactaa attaggaaag ataatgccag atactcctgc
     1981 acaggctgta gggcttcaag taggagataa gataaataaa attaatggta attctgttaa
     2041 gacttgggat gaagttgcaa atataataaa cacttcttct ggtggagaat taaaacttag
     2101 tataactaga gatggaagta ataaagttgt aaatgtaaca cctaaaaata acaatggaaa
     2161 gtatgaaata gggattcaac cacaaagaga aaaagacttt ttggcgtcaa tagtaaatgc
     2221 atgtaaaact actgtagata tgacaaaaca gatgttaaca tttctaggtc aaatgattac
     2281 aggacgtgta ccaggaggga ttggcaatgc tgtagcagga ccagtaggtg ttattggtat
     2341 ggtatcagat gctgctcgta caggcatcat aaatgtagtg tatttagcag ctgtcataag
     2401 cttaaattta ggtatagtaa atctattacc aataccagca cttgatggat ggagaatact
     2461 tatgctatta ctagaagctg ttagaggtgg taaaaaactc gacccaaaca aagaaggcat
     2521 gataaatgtt gttggttttg gagctttaat gttatttatg ctttttatca catataaaga
     2581 tatattaaga ttatttcaat gatttagttt tttagagatt ttaagaaaat aggctatgta
     2641 gttttaaata agaataaatt aatttttatg tatgaaattt attcttattt aactagatag
     2701 ctttttaatt tataatatac atgtaggtta taatatttta ctatttaaag agattcaata
     2761 taaaataata tatagaggtg gaatatgagt tacgagagaa gaaagacgag agaagtaagt
     2821 gtaggaactg taaaaatagg aggagaaaat cctataagta tacaatctat gacaaatact
     2881 gatacaagag atgcagatgc tacaatagct caaataaaga gactagagga agctggttgt
     2941 gatatagtta gagttgctgt tcctgatatt aaagctgcta agaatatagc aaagataaag
     3001 tctagtgtaa atataccaat tattgcagat atacatttcg attataagct agcccttgaa
     3061 gctatagaac aaggtgtgga tggaataaga ataaatcctg gtaatattgg aagtagagag
     3121 agagttaaga tggtagttga gaaatgtaag gaaagaaatt taaaaattag aattggagtc
     3181 aatggaggtt ctttagagaa agaactttta aaaaagcatg gctctgcaac tgctgaagca
     3241 ttagttgaaa gtgcaatggg acatattaag attcttgaag acttagattt tcataatata
     3301 gttatatctc taaaatcatc cgatatttat aaaactgttg atgcatatga attaatatca
     3361 aaaaaagttg attatcctct tcatattgga attacagaat ctggaagtgt tcacaaagga
     3421 acaataaagt cttctatagg ggtaggagca cttcttctta aaggaattgg agatactgtt
     3481 agaatatctt taacaggcga ccctgttgaa gaggttattg ttggtaaaca gattttaaga
     3541 agtttgggac ttttaaatga taagattaaa gttatatcat gccctacatg tggtagatgt
     3601 aatatagact taattagtgt tgtaaatgag gttgaagaaa agattggtag tatggaaaaa
     3661 aatatcactg ttgcaattat gggctgtgct gtaaatggac ctggtgaagc cagagaagca
     3721 gatataggta ttgctggggg taaaggtgaa ggtcttttat ttaaaaaggg tgaaatagtt
     3781 agaatgattg atggtaacaa attagttgat gagttgctag aagagattga aaaattatag
     3841 tatacaattc atatatatat taattgacta gatttttaaa tacttttgta ataagttttt
     3901 atttacagta tttaattatc tagtcatttt ttattttata aacttcatta aatgtaagaa
     3961 acttatattc taatttgact atatatgtat gagttaaatc atattgtaaa tttataaaat
     4021 taaaaaaaga tttaatatag tgaaaatttc catcaatagt attgaaactt gatagtaact
     4081 atgataggat atagttacta gaacagttgg taaattatgt taacatagat tttaatgttt
     4141 tggtaaattg ttttattata agttaataga aaaagaggtg aagaactgtg agatataaaa
     4201 aaatatctag cttacctatt ttagttgttg ctttgctgat tactggaacg acaacaacat
     4261 tttctcttag tacgaccaat tcagaaaaaa caaatgtata cttagcaagt acggtaagta
     4321 ctgtcacaaa gaaagtatcc gatattcaaa gtagtaaagg tcttgtttat aatgagaata
     4381 ttccattatt tataaataat agcaaaatac agtatgatga tactccatat cattggccta
     4441 gcaatgttat aagcttaaca aatagcagtg aaaaggcaat tatggattat gagattacat
     4501 gcttagcata tgataaaaat ggaaaaccac ttgagctata ttgggatgct caaaatgtgg
     4561 cagcagatgg cgaagtagga agtgttggat ttagcccagc tggtgtagac tatggtattg
     4621 ttacaggcat atctcctgtg tctccaaaat catattcaca tacttataga aaaatgcaac
     4681 aatcaccacc acaggatata ataagtatgt ttgaaaaaca acaaggcaag gcatgggtag
     4741 aaaattggtt gaaagaatgg aaacaaatgg agaaagaata tgcaaagcaa aatgcaatag
     4801 caccaggtaa gaatcaaaat gatgcctttc tattgtttga taaatggaaa caatcaactg
     4861 gagagcatgg tgtaaagtac attatatctt gtgtaaaaca agtaacattt aatgatggta
     4921 gtatatggaa aaattcagca tatgagaatt ggttgaaaag ttttcaaggg aaggaagttt
     4981 caaatagtgt attagaaaat tactataagt aatgtttaat gatagcaaaa tgagtacgaa
     5041 tttaattaat gttaatttaa ttaaataagg actattttgt ttaaaaatat cagtgttaca
     5101 aggaaatttt tattgtgaca aaatggtata tattcaaaat aaaaaagcat gtgtcaagtt
     5161 ttgatacatg cttttttatt ttgaatatat gttttctata aatttatgat tttttagtta
     5221 tgtaatttta tatgtgaatt tttagatttt tatatagata gtgaaataat gtggaagttt
     5281 tatgttttaa aaattttagt taaaacattt attaaaaaag ataatagaaa tgttttaact
     5341 aaaatagtta gaattgaaat gttatacaga tagtataagt actatcaaat tatataagat
     5401 tgcttgaagt taaataattt atttaaatag aaaacagctg atttaatatc agctgtatat
     5461 gaataaaatt ttgaattgta gtgtttagtt atatggatat aattcataat aagttagcaa
     5521 taaatcaacc attttaccat aactctgagt accttcagta acgccattag cttttaagta
     5581 agaattgtta aactcattag aaatttcatt aattttacct tcatattttt gccaaaattc
     5641 actttcattt tttaaatctc tacggacaga atctgaaatg ccagcagaaa tatcaacata
     5701 tgcattataa tcaaccttac ttaaagcaga agcagtataa tttaaagcta aaatatatcc
     5761 agaatagtta aaatcaatat gggggtgttt tatagaagta agatacgcta taaaattagc
     5821 ctcatcttca ctagcaaatc ccctttgatg tgctatttca tgttcaactg tacaaggaat
     5881 atataagtca ggtattgcta tatttacatt tgcctcacct gtaaaaggaa agtatatacc
     5941 agttattcct gtatagcaca ttagatttga tgaaataata tacttaggct tagaataact
     6001 accactaaca ctaggtaata tatccaagat attatcgtat cctagttgag ctctatttat
     6061 gacaccatta taatctgtat tagattttac aataccattt ttatcttgta atgtgagttt
     6121 tctagtttca tttgctttaa ttacaaggaa tttatataat ttagtaagct ctttaacact
     6181 atattgtttt gattggatag aggtattgtt ttttaaatta tagttattta taagtgtggt
     6241 ttctagtgga atcctattat agtttattcc ccaaagcact acaaaaagaa agtagactat
     6301 tgatacaatg gataatatat ttaaaatgga attttttaaa aatatgttta attttttttc
     6361 ttattaaaaa ttgtagataa agtacaacat aaaaataaga agatagatat aactattatg
     6421 tacattgtaa tctcatagag agaaaatggg aaaattccag aaactttact caatatttgt
     6481 acaattattt tatctatgaa ttgggagtaa tatttttcaa caatatttgg aactttgctg
     6541 gctataaaat ttaaaagtaa agcaatagga aagagaatta aagaaaaata ttttgttttt
     6601 ttgctcatat ttcacctcac atacattagt ttatataaac atattatata tcaattgaag
     6661 aaagattgta aaataagtat aaatatatct atttataact ttatactaaa taaatgataa
     6721 gttataatat attaatatga ataagcagta tatatacaac taataataag aaaaatatca
     6781 attatataag gagtagataa aaatatgata ataaaactta ctaaagacaa ttttaatttt
     6841 agatttaaac aatatgaagg tatgtttttt catgctgaag tagttgaagg tatggaatcc
     6901 attagatata aaataactaa ggataaagaa ggtacagaat taaaagaatt tggaagtaca
     6961 catataaaag ataatatgat atatgtacct caggtagaag catacataga tttgaacagt
     7021 ataaaataaa gatgtaaaat tttaattaaa aataaagatt atgtttgagg gggaatccaa
     7081 tatgagtaag cctatagata taacaataag acctataaga agagaagatg cacaagcttt
     7141 taatgaaatt agaagaacat acagtgttat gagaaatact ttggcattaa tgagtgacag
     7201 agtcgataaa actgaagcta tgttagcttc tttgggagaa aatgattata tgtttgtagc
     7261 tgaaactaat aataatggag aaaaagatgt aataggtttt gctgggttac atgtaaattc
     7321 ttctccacga ttgagacata gtgcagaaat aggaataagt gttgcagaga attatcaggg
     7381 aagaggagta ggtaagaagc ttatggaaca acttttagat atagcagata attggattat
     7441 gttaactaga gtaggattag aagtaattgt agataatgaa aagggattaa atttatataa
     7501 aaaactaggt tttgaaatag agggaactaa aaaatatgct gtaatcagag atggaaaatt
     7561 tgaagatgtt tatattatgg gaagatataa taaaaattta atttaaagaa gctaatagag
     7621 aagtaaaata gagtgtcttt aataaaaaat ttacattaaa aataatctat tttacttctc
     7681 tattgtatta aaaaacttat ttttaaatgt ttacaaaaac attctatata caaataaaat
     7741 tattagcaat atcaacgcta ttttaaaagc taaactagca acagttttaa tgatttttac
     7801 agaaacacct atagcaacta tagcaaatac tattttaaag agtaaatcca tatccatatt
     7861 taagcctcct attctataat acatatagat attattgaca aatatctagg aaaaaattcc
     7921 atcaatatac tacaataaaa tgtttatatt tgtaatttat tgaatatgtt attatataat
     7981 acataaaaaa ataaatcaag ataagagtaa aatacttata aaaggtatag atataactga
     8041 aaatagtgtt gtaaaaaaca cacattgtga agctagtgtt atattggagt catattgatt
     8101 agccattata gcagtatttg cagcagcagg cattgaagaa attactactg gtatagctaa
     8161 aatcatctta tcatttatcc atccttttag aataaaatac acaatcacag gaagtaccaa
     8221 taatcttata aaagtgacta tatataattt tttgttgaca aaacaatcga gagctgaaga
     8281 attagcaagt aagcttccaa tgataatcat ggatattggt gtagttatac ttcccaacat
     8341 ctcaagtggc tcgtttataa attggggtaa cctaagtcct gttacaaata aaaaaagtcc
     8401 aataacaata gctattgtag cagggcttat aagtgatttt attgaaaaac ctctacttgt
     8461 actgccttta gataaaagat aaattcctaa agtgaaagaa aataagttaa aaggcaaatt
     8521 gaatattgca gtatagaata ttgcttcatg acctaaaact gcttcaatta caggataacc
     8581 cataaaagca acatttgaaa aaacaactat atactgatat attcccaaat cttttttatc
     8641 attacatttt aaaatgtatt tcaataaaaa tgcaattata attgaaacta tatacataat
     8701 caaagatgta aataataaca atagtatttt ttgtatcatt ttactatcaa agttaatttg
     8761 catggagcta attatcattg atggtaaaaa aactgtcatt gtcaaagttg aaagcttaga
     8821 tgtgcagtgg tcatctaata agttgaattt tctgacaaaa tatccaacta aaataattat
     8881 aaataaaact gcaacttgga taaaaatatt gctgaaattc ataaaatgtc cccccctata
     8941 tatacattat ataccaatta cataataaaa tctaatgatt ataaaattta aaatttaatg
     9001 ttacaatata gtgacaaaag gtaaaaattc aatattatag cattaattat agattataat
     9061 gtataataat gtttgagaga atatatttta taaaatgggt aggtagcgaa taatgaaaaa
     9121 tacaaaattt tctataaaaa aaataaaaaa tatatctaat gacccgaaag tcgtacatac
     9181 taagggtgcc ctccttgaaa agatgggtaa gatggaaagc gcaatagagt tgtataagag
     9241 tgctgcattg gataattacg taaaatctca atatacttta ggaaatatat atgaaagtaa
     9301 aaaacaattt aaagaagcag aaaagtggta ttcaatggca tataaaagtg gaagtgaaga
     9361 tgctgctttt gatttaggta atatgtacta taagttagat ggctatgaat atgctatata
     9421 ttggtatgaa aagatagcaa atttaggtta tttacaagca caaaataatt taggtgtatg
     9481 ccattttaaa atgaaagatt ttgtaaggtg tgaaaaatgg ttaaaaacag cagcagacaa
     9541 gaatttaggt aaagcttgtt ttaatttagg ggtattatat acttttttgg agaaagatga
     9601 tgaagcatat gaatactata aaaaaggttc tgtgctttta gatgatgatg caaaatataa
     9661 tttagcaata ttaaatggaa aaaagaaaga taataaatca acagtaagtt tatataaaca
     9721 gttgtacaag tcaggtcata tgaaaggttg ctttaacttg ggtatgatta tggaaataaa
     9781 tgataattta gaagatgcag agaagtatta taaaaagagt gctgatagag atgatgtgaa
     9841 gtctgagtat aggcttgcat atgtatatga tagaaaagaa gagctgggag atgcaattta
     9901 ttattatgag aaagcaatag aaaaaaatca tacactttct aagtatagac ttgctaattt
     9961 atataataga gaaaatgaga tagagaaggc aaaaacttat tataaaatgg ctgctgaaga
    10021 tgatataaca gaagcaaaaa ataatctggc aggtatatat tttgaagaaa aagattatga
    10081 aaatgctatt aaatattatg aggatgctat agctgttgga tgcaaaagtt cccttgaaaa
    10141 tcttggggat ttatattatc agaatcaaga tatagaaaaa gcgatttcgt attattcgag
    10201 gattccaaat aatgcatctt gtcaaatcaa attaggaaat atctatgaag atttaaataa
    10261 tattgaagaa gccataagtt ggtataagaa agcatctgaa aatggagata cgaggtctag
    10321 ttatagattg gggtgtatat atgagagctt aggaaataca aaaaatgcta gaaagtattt
    10381 tgaaatggca agtagcaaaa atcatatgaa tgctcgtata catctaggta gaatttattt
    10441 tagagaaggt aaattggaag agtctaaaat gatgtttgat acacctgcca atgaaaataa
    10501 tgtgtacgct caacatatgg taggattaat ttatgatatg ttctataaag attatgtaaa
    10561 ttctaaattt tggtatgaaa aggctagagc acaaggttgt gttgaatcaa tatataattt
    10621 aggccaaatt tatttaaaat taaatgatga cgcagaagct gagaaatatt ataaagaagg
    10681 tattaaatat ggaagtaaga agtgtgagta tatgcttgca ggtctttatt ataaaaagag
    10741 cctagatatg tacatttctt tagcaaatga agagtacgat aattgtgaag agatagtaga
    10801 aggctttccc aatttaaata tagactttga tgaagtatta attcctccat ttaagttgca
    10861 agaagaggta atagatgaag aagagtatgt accaatttat atactaaata taaaagaaag
    10921 tttagattca atgctagaag ggcttgaaac tgaaatgatt atagatgaag aacatgatag
    10981 ttaaaaagta ttataagatt atatagattt aaatatataa aagccattag gataaatgta
    11041 aatcaattaa ttctaatggc ttttttatta atattttata atagcttatt tcttgacatg
    11101 aaaaatgtaa tcactataca tattatagag gtaataagaa gtattatcca aaatccgttt
    11161 gggttattag aaaatggcat gttgatgaca ttcataccaa agaatcctga tattattgta
    11221 ggtattgaga aaattagtgt aactactgtt aaaatttgca taacattatt ctgattatta
    11281 cttataattg cactgaaagc atccataatt ctactcaata tatctccata tattttagcc
    11341 atatcaagag cctgtctatt ttcaactata acatcttcta ataggtcttc atcatctgga
    11401 tacttcttta taccagcagt tctaaccatt ttgtttaaaa caagttcatt agattttagt
    11461 gaggtagaga agtataccaa agatttttct aattccaata attgaaccaa ttctttgttt
    11521 ttcattgatt tgtaaatttc tcgctctatt atgattgtca ttttatttat ttttcttaag
    11581 taatgcaagt aataagctgc atttttgtga agaatttgaa gcaaaaagcg agtcttttta
    11641 aacgtaaaaa aatctttaat atgaccaact ataaagtcat ttaaaatctt agtctgtgca
    11701 tcacaaactg ttacaatagc ttcttcagtt agtataatac ctaagggtat tgtagtataa
    11761 tgagaagaat tagagtcact ttcgtctact ggtatatcta taagtataag agtatgattg
    11821 tcttcaacat ctattcttga gcgttcttct tcatctagtg cagattctat gctttctaca
    11881 tctatattta ataaattaga aatttcgttg atttcactat gattaggttc aacaagattt
    11941 atccaacagc catcttcaaa aaaactaagt ttctctaatt ttgaatctat agttttgtaa
    12001 tatcttatca tggcaacaca tcctttattc agttttatat aaatctcatc atttaatagt
    12061 atctattaat taactaattt atataactga atatatgtat tagttttgat tttagatatt
    12121 attaagtgat gaaaatgtaa aaaattctct ccgcaacgga ctagtatcca ttgcccatca
    12181 ctctccttat ttattatttt atatatagct atttatgaaa tgcgtatttt aagcactctt
    12241 taatataata ccttttccag gtatataaat caagttttga tttgattaaa taatattttt
    12301 accataattt acacataaag tatataagtt atattta
//
LOCUS       DAGQWR010000123        12327 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.123, whole genome shotgun sequence.
ACCESSION   DAGQWR010000123 DAGQWR010000000
VERSION     DAGQWR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.123"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            228..1157
                     /gene="glsA"
                     /locus_tag="MIW95_002988"
     CDS             228..1157
                     /gene="glsA"
                     /locus_tag="MIW95_002988"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBY3152296.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            1255..1818
                     /locus_tag="MIW95_002989"
     CDS             1255..1818
                     /locus_tag="MIW95_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3152297.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            2306..3142
                     /locus_tag="MIW95_002990"
     CDS             2306..3142
                     /locus_tag="MIW95_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBY3152298.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHIGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            3394..4389
                     /locus_tag="MIW95_002991"
     CDS             3394..4389
                     /locus_tag="MIW95_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBY3152299.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRNKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(4646..5050)
                     /locus_tag="MIW95_002992"
     CDS             complement(4646..5050)
                     /locus_tag="MIW95_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBY3152300.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     FLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            6050..6964
                     /gene="queG"
                     /locus_tag="MIW95_002993"
     CDS             6050..6964
                     /gene="queG"
                     /locus_tag="MIW95_002993"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBY3152301.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFVDNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEMPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            7070..9307
                     /locus_tag="MIW95_002994"
     CDS             7070..9307
                     /locus_tag="MIW95_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY3152302.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNRLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDKSDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNESSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            9504..10556
                     /gene="nth"
                     /locus_tag="MIW95_002995"
     CDS             9504..10556
                     /gene="nth"
                     /locus_tag="MIW95_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBY3152303.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKEIDKMISKKDEIIDEKDIENFRYIEEDKKSIKVSKKST
                     KKGETEKKTKKSSSNKATQKQSKEVKSKSKIKSESANKKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            10750..11220
                     /gene="trmL"
                     /locus_tag="MIW95_002996"
     CDS             10750..11220
                     /gene="trmL"
                     /locus_tag="MIW95_002996"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBY3152304.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            11256..12104
                     /locus_tag="MIW95_002997"
     CDS             11256..12104
                     /locus_tag="MIW95_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBY3152305.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVDEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
BASE COUNT         5058 a         1128 c         2293 g         3848 t
ORIGIN      
        1 gttaggttag aaatttttga cgttataaga atttaaatta ctggattatt agattactaa
       61 attactagat tattaaatta ttaagctatt taaactgttt aaattgtttg aaaaatgaat
      121 tgattcttgt agaaaatagt tttaaagtat aatttttttt aacactgttt tattatagta
      181 caaacttgtg ctaaaataga atatataaca taatataaga ggtgcatatg aatacgttag
      241 atgagacatt attaaaagaa attataagtt ctaataagaa gtatactaat tatgggcaag
      301 tcgctagtta cataccagaa ttaaaaaacg ctagaagaaa tgatttagga atttgtatca
      361 tagatagcga aaataatctt tatagtgctg gaaactgtag cacaaagttt actatacaaa
      421 gtatttcaaa accaattgtg cttgctatgg cacttatgga taatgattgg gaagacgttt
      481 tttcaaacgt tggaatggag ccaagtgggg acccatttaa ttcaataatg aaacttgaaa
      541 ttaatgacac aaaaaaaccc tgtaatccaa tgataaatgc aggggctata gtaactactt
      601 cccttataaa tgggagttgt ttagaagaaa aagaagaaag aatgcttagt ttctttagaa
      661 agttagcaaa aaatgataat ataggaataa actatgatgt ctataagtct gaaaaaatga
      721 ctggagatag aaacagagct atggcttact tattgaaaag tgatggattt atcagaggga
      781 atgtagaaga tgttctagac ctttatttta aacaatgctc aattgagata gattctgtag
      841 atttagcgag aattggaata aatcttgcaa attatggagt agatatagaa aatggtgagc
      901 atttaatgag tgaaatggtt tctaggattg tcaagacatt tatgatgact tgtggaatgt
      961 atgatgcttc tggggaattt gctattaaag ttggcattcc tgcaaaatct ggagttgggg
     1021 gtggtataat ggcttctgtg ccgggacgca tggggattgg tgtttatgga cctgcgcttg
     1081 acaaaaaagg taacagcgtc gcaggagtta aagttttaga ggagttgtca aataaactca
     1141 aattaaatat tttttaaaat attaagaaag gatttttttg acaacaacat ataatttaat
     1201 atataattaa attgtaaaga aatatgaatt aaataaattt tttaggaggg gtcaatgaaa
     1261 gaggtattgg tattacgaga tttggagtgc ataaaggcta ttgcgcatcc acgtagaata
     1321 gatattctaa aagcatttga caaatcgcca ttgtcagcaa aacaattatc tcaattattg
     1381 gaagaacctc atgcaaaaat aaactaccat ataaaaatgt tatacaaagt tggtatttta
     1441 gaattagttg aggaaaaaat aaagtcgggg attgtggaaa agtactatta tccaagtgca
     1501 aaaaatgtgg ttatagggaa taggatactt aatttttctt tagacaatgg ggaagaaaaa
     1561 gaagaattgt acatatcaaa atttgaaaat atgagtgaag ttttttataa ggcagcagag
     1621 gaagatgttt tagaaaatga aaatatagtt gattatcatg atatttctct tactcatgat
     1681 gaattagttg aacttagtga cactatgaag tcaaagatag atgaaatttt aaataaaaga
     1741 caacacaatg ttgaaggttc taagtatgat atagctatgg ttatagttcc tactcttgag
     1801 gaggaatgtc ctagttagag aattaattat ggattaatta aagatataat attagagatt
     1861 gaacatcaaa atttacttaa taatcaaaaa atagtttttg gtatgggtaa aacaatgatt
     1921 tttaatctct atttttttgt tttcttatac ctattgtatt tgtaaataat atgtatagtg
     1981 taagatgtat acactatctg agaagtgctt agataatata caaagtatgt aagattataa
     2041 tatttgaaat tgattaaaat gggtataatt aaagtgtaaa tattaaaatt aaaaattaat
     2101 ataaattaaa tcaatgctag aaatatgtta aaagaggaat gagattagtt gggatagaac
     2161 atcccttaga gcttatgaat ttttagtgaa aaagtactat caaataggaa tctccatctt
     2221 catatgttgg ctaaatatag ttaaggtgga gtagttcaaa attaaaattg agtacaagtc
     2281 gtagtacaaa taaaaggaga ttaatatgat taaattagga acacatcttt caatagcaaa
     2341 aggatttgct aatgcggctg agacagctgt atatattggt gcaaatacat ttcaattttt
     2401 tagtagaaat ccaagaggag gaaatgctaa agaatttgat gaaaaagata taattaaatt
     2461 tcaagaaatt agaaaagaaa ataattttgc accactgctt gcacatgcac catatactat
     2521 gaatctgggt ggtacaaagg atgatgtata cgaatttgca acaagggtga tagatgagga
     2581 tataagaagg atggactcat taggaattga atacatgtgt tttcatcctg gaagtcatat
     2641 aggtggtggt gtagattttg gtatagatag aatagtaaaa ggattgaaca atgccataaa
     2701 gggtgatgaa aatataacta tacttcttga aactatgtcg ggaaagggaa ctgaaatagg
     2761 ttttaaattt gagcaattaa aaagtataat agacaaaatt gagcataaag aaaaaatagg
     2821 tgtgtgtctt gatacatgtc atattttttc agccggttat gatatagtga atgatttaga
     2881 tggggttttg gaagaatttg ataaggttat aggcatagaa agattgaaaa ctgtccatct
     2941 aaatgatagt atgatgccat ttggagacaa aaaagacaga catgctccta taggggaagg
     3001 aaagatagga ttaaaagcat taattgattt tatggaacat cctagtctaa aacatttacc
     3061 atttttctta gaaactcctt ttgatgatga aggtcacaag agagagctta aaatgataaa
     3121 agaaatacta tctagtaaat aaacctttat taaaaatatg tgaaatactc cgccctcaac
     3181 ataattaagg tggagataca atcatgaaat gtgataattt aaattaatgg ataggaataa
     3241 caaaaattgt atacaaatta actgggtata aagtatgatt atattatgaa tagtatgtaa
     3301 atatattgca aagattctca taatttgttg tataattaaa ggtaacatat ttatattata
     3361 taaaaaatta ttaaaagtta ggtggggaaa gatatgagaa atttaggcaa tactaacatg
     3421 aaaataaaga gagttggctt tgggggcatt ccaatacaaa gaattactca agatgatact
     3481 aacttagtta taaatgagtt agaaaaacaa ggaataaact ttattgacag tgcaagaggg
     3541 tatacaataa gtgaggaagc aatcggaatt gccattgaag gaaaaagaaa taaatttttc
     3601 ttagctacta aaagtatgtc cagagattat gattcaatga agagagatgt tgaaataagt
     3661 ttaaataatt ttaaaactga ttttatagat ttatatcaat tccataatgt taaagaagag
     3721 gagtatgata atctttttaa agataaaatg gcttatagtg cccttttaga ggcaaaggag
     3781 caaggtaaga taaaacatat tggtataact agtcacaatt taaatactat agaaaaagct
     3841 atagaagatg gaaaatttga cactatacaa ttcccttaca atatagttga aggacaagca
     3901 gatgaagtgt ttaaaaaagc tcatgaaaaa ggaattggga taatagtaat gaaaccttta
     3961 gctggaggtg ctttagacaa tgcaacactg gctattaaat atatactatc taaagattac
     4021 atagatgtag ttataccagg tatggagagt gttgaacagg ttagacaaaa tgtcgctgta
     4081 cttgaaaatc ttgttctaga tgaaaaagat aataaggaaa ttgaagaaat aagaaattct
     4141 ttaggtaaaa aattctgtag aagatgtgaa tattgtatgc cttgtgctgt aggaataaat
     4201 ataccattga gttttttatg tgaaggttat tatactagat atggattaaa agagtgggct
     4261 aaagaaaaat atgaggttat ggatgttaaa cctactgagt gtattgactg tgggctttgc
     4321 gagagtagat gtccatatga acttcctata agagaaatgc ttaagactgt tgttgaaaag
     4381 ctaggataga ttttatttat tgaaatcggt gtattaaatt tactccgatt tctttttatt
     4441 agatgtggtc ttatatacaa atattaaatt tacatagtta tagctattat ataaatttaa
     4501 tatttgttta atacttatta aatatttgct taatgctttt taaaaacatt tgtttgagta
     4561 tagacataac acaattatta ttgttgtatt agtatagttt caaatactgg agtatcaata
     4621 taatgtcgaa taacacataa aatctttatt tttcaaaaat attataggat atatcgtatt
     4681 tagatactgg ataaagttca atttcaaccc tagggttatc tttatctata aaatcaaata
     4741 aaaatactgg cttaagttgt ttatcatttt ttattattcc acttttttca ataccatcac
     4801 aaatactttt tggataatta tgtaaatccc ccattctctt atttggaaag aataatttca
     4861 agactgttat tagattttct tcaacagttt ccccttgata ctgttggttt ataaaacctg
     4921 caatcatgtt ttcgtatgca agatacttag aatattttcc ctttgaaggc atccatgctt
     4981 gaccatttat gttatgaagc ttaaaattag atttactaat aggtctacct ggaattgtta
     5041 ttttaatcaa atttttcacc atccttatac agtatttatt ataatctgta tatgatttaa
     5101 aagcaaattg ttatttaaag tgtttttaca gggcttgttt gtaaaatttt ccactttaga
     5161 tgtagtaata taagataagt attttgctta caaattttaa aatttgatat atgatggaca
     5221 tattcatatt agactattat aagaccttca taatattaat aagtataggt ttagtaaaga
     5281 gagtaaaaag aaatccatat aagccaagta aagacttaat aaatggcaat gtaaatggga
     5341 tattaattat aagaaaaaat caatcttttt gcattaacta taggaagaat gtcaaaatat
     5401 aactaatgat atttgaagtt ttagagttta tattaaaagg taagaaggtt aagtgggtaa
     5461 aaaaggttga atagattaaa aaggctaagt atctaaaaga gttaagtagg taaaaaagtt
     5521 aaataagtaa atgaaaaaag ctaagtatct aaatgagtta gtagataaaa aaattaaata
     5581 gataaaaaga ttaagtatct aaaagcgtta gtagataaaa aaattaaata gataaaaaga
     5641 ttaagtatct aaaagcgtta gtagataaaa aaattaaata aataaaaaag actaagtatt
     5701 caaaagagtt agtaggtaaa ataattaaat agataaaaag actaagtatt taaaggagtt
     5761 aagcagataa aaaagttaaa taagtaaatg aaaaagacga agtatctaaa agagttagta
     5821 aataaaaaag ttaaataagt aaataaaaaa gatgaagtat ctaaaagagt tagtaggtaa
     5881 aaaactaaat aggtaaaaag acgaagtatc taaaagagtt agtaggtaaa aaagttaaat
     5941 aaataaaaaa gacgaagtat ttaaaagagt tagtagctag aaaaggttaa aaagactaaa
     6001 aaaattaaat aaatttaaaa agtcaattag attaaaaagg aataatttta tggataataa
     6061 ggaattaaaa gcattttgta atttaatagg attaaactgt gtaggagtag caggggtaga
     6121 taagtatgat aatttagaaa agatacttaa ggacagacaa caaaaaggtt atttaactgg
     6181 tatggaagaa ccaataattg agaacagaat taacccaaga catataatga aggatgctaa
     6241 ctctataata gtctgtgcat ttccatatca tataaataga caagaaaaga ataaaaattc
     6301 aaatttatca aaatattgtc atggaaagga ttatcacatt gtagtaaagg actttttaca
     6361 acaaatttgt gattatattt ctaaaacagt aacagatttt aaatacaagt tatttgttga
     6421 taatggacca ttagttgaca ggtatttagc ttatttatca ggaataggat attttggaat
     6481 aaataataat atcataactg atgaatatgg ttcttacgta tttatagggt atatagtaaa
     6541 taattataaa tttgaagaag ataaaccact agaaaaaagt tgttttaaat gtggtaagtg
     6601 tgtaaaatat tgtccgggaa atgctatatt gggaaattat gatatgaacc ccaaaaaatg
     6661 tttatcatat ataactcaaa aaaaaggaga tttgtcagag aaagaaaaag ttgttttaaa
     6721 gaatggcaaa aaggtatttg gatgcgatat ttgtcaagaa gtatgtccac ataatgttga
     6781 aatgccaaca actcatattt tggaatttaa agaaaatata atagattatt tggattatga
     6841 tgagatacaa aatatttcaa ataaagaatt taaaaggaga tatggagata gagcttttag
     6901 ctggagaggg agaaatataa taaagagaaa tatggaaata attttagata agtatgatga
     6961 ctaataataa gagtctgttt catatacttt aatgtgtatt tactttaaca cttgttgtgt
     7021 aaaagtcata ttatatcatt aaagttaaat atggggaggt gggggaatat tgaaatataa
     7081 taatacatct gaaataaatc cactagatta tatattgggt caagaaagag ctgtagaagc
     7141 tatggaatta gggttgaaaa taaataatcc agcgtacaac atatatatag ctggtgaacc
     7201 tggaactggt aaaagtactt atgccttaaa ggttttaaat gaatatgcat ccaaaaaaaa
     7261 tacacataaa gattggtgtt atatatataa ttttgaaaac cctagagagc ctattatagt
     7321 tgaattagaa aaggggtttg gaagagaact taagaaagat atggagaaat taattgaaag
     7381 tcttttagaa gattttaaaa atgcgtttga aagtgaaaat tttgaaatag aaaaaaatag
     7441 acttctagat gaatatgaaa tagaaaaaga tatgcttttg aagcaaatta agaaatatgg
     7501 ggaagaaaaa ggatttaaat taaaacagag taaaatggga atagttttta tccctttgaa
     7561 agaagaggaa gatgaaagtg ataagtctga tgaagagttt tacaaagcta aaagagaact
     7621 agaaaatatg gcgatacagg tcgtatataa aattagaaac ttagaagaaa tagctgaaaa
     7681 agcagtgtta gagcttgaag aagagatagc taaacttgta gtagaacctc atatcaagag
     7741 attgtgtgaa aaatatgaaa attatgataa gattcaaact tatctggaaa atataaagaa
     7801 agatataata gaatatatgt atttgttcta tttagatgaa gaggagttaa aagataagta
     7861 tgataaagag cattttatta aatacaaaat taacctattt gtagacaatg aaagctcaaa
     7921 gaataaagaa tcagcacctg ttgtagtaga gataaaccca agtccagcaa atctatttgg
     7981 aaaggcagag tatgattatg caaatggaaa tataaagact gatttcacaa aactattatc
     8041 aggagcattt catagggcta atggtggtta tttagtactt tatgcagacc aattactaaa
     8101 atatacaatg tcttgggaaa ttcttaaaaa gactttgcag actaaaaaag taatattgga
     8161 aacacaaaca gcaattaaac cagaaaatat gccattggat gttaaattag tactgattgg
     8221 aagccactat atatatgata ttttatatag atatgatgaa gattttgaaa aatattttaa
     8281 agtatttgta gactttgata gtgaaatgga taaaaatgaa gataatgaag aaggaatagc
     8341 aagatttata gcataccaat gtgataagaa taatttaagg cattttacta aatcagcagt
     8401 tgaagaggtt attaaattta gcactagatt atctggtgat ttagaaaaac tatcaactaa
     8461 gtttaataaa ataatggaag ttgtaataga aggaagtgct tatgcagagt ttagaaattc
     8521 ggagtataca gagcagtgtg atgttaaaaa agcaataagt gaaaagagaa aaagaatcaa
     8581 tagagtggaa acacatatgg atgaatcaat tgaaaatgga tttactctaa tagaaacaga
     8641 gggaagaaaa gtaggagtga taaatggtct gtcagtctta agtacaggag aatactcttt
     8701 tggtagagct tcaaggatta cagcgaccac tagcccagga agtaaaggca tagtaaatat
     8761 agaaagagaa gtaaatatga gtggctctat acataacaaa ggagtgctta tattaggtgg
     8821 ctatctttct gagaattttg cacaggattt actattgtca ctaaacgcat atgtttgttt
     8881 tgaacaaaat tatggtggaa tagatggaga tagtgcatct tctgcagaat tatatgtact
     8941 attatcatct cttagtggag tatcaattaa acaaaatatt gctgttacag gttctataaa
     9001 tcaaaagggt gatatacaag tagttggtgg tataagtgaa aagatagaag gattttattc
     9061 tatatgtaaa actaaagggt taaatggtga gcaaggcgtc ataattccta gaaaaaatca
     9121 aagaaatctc gttttatcag atgaagttaa tgatgcagtg cgagatggaa aatttaagat
     9181 atatactgtt gaaagagtag aagaggctat agagatattg acagatgtta aatttgaaga
     9241 aataaagaaa ttggtaaaag aaaaattaat atctttcagc aagatacaaa ctgtgtcaaa
     9301 agaataagtt tattatagaa tttaatgggt atttatgtat aataaaaagc aagaacttta
     9361 ataggttttt gctttttttg taaatatata taaattaaat tcttattttg tatgttttat
     9421 atattgatgt ttattttttg ttgatatgac taaactattt ataaatagtg ttttataaat
     9481 tggtatttta aagggggata attatgaaaa atgtaaatct aaaagagata aatgaaaaag
     9541 atgtaattgt ggaagaagat ataaatctaa aaggaatgga taagaaagat gtaattgaga
     9601 aggaagaagt aaataaaaaa agtatagata agaaagatat aattgagaaa gaagagatag
     9661 ataaaaaaaa tgtaaagaag gttgaggttc aaaaagaaat agataaaatg attagtaaaa
     9721 aagatgagat aattgatgag aaagatatag agaattttag gtatattgaa gaagataaaa
     9781 aaagtatcaa agtaagtaaa aaaagtacta aaaaaggtga aacggagaaa aaaacaaaga
     9841 aaagtagttc taataaggct acacaaaaac aaagcaaaga agttaaaagt aagtctaaaa
     9901 ttaaaagtga atcagccaat aaaaaagatg taaataagat tttagatgag ttggaaaaat
     9961 tgtacccaga tgctaaatgt gagttaaatt atgggacagc ctttgaactt ttaatagcaa
    10021 ctattttatc agcacaatgt actgatgtaa gagtgaataa agttacttca gagttgttta
    10081 aaaagtacaa cacagctaga gactttgcta atttgagcat tgaagaaatt agtaaagaga
    10141 taaagagttg tggtctttac aaaagcaagt ctcaaaaaat aaaggataca tctgaacagc
    10201 tatgtgaatt atatgatggt gaagtacctg atagcttaga aaagcttata aaattacctg
    10261 gtgttggaag aaaaacggca ggggttgtac ttagtaacgc ttttaatcat ccagcaatag
    10321 ctgtagatac tcatgtattt agagttagca atagaatagg aatagtggac gagccaaatc
    10381 ctcaaaaaac agaatttgca cttatggaag ctataccaaa agaaagatgg tcacactcac
    10441 atcatgtatt aatttttcat ggaagaagaa tgtgtaaagc tagaaatcca gaatgtgcaa
    10501 gttgcccaat aaaagaagat tgtaattatt ataaagaact taatgaaacg aaataacttt
    10561 tagaaagtaa gttgtacaag agggattttc tgatataata atacttgtga aaatgttaga
    10621 taaaaaacta ttatatacat gaaataaaaa ttattattaa cattatttta tataatagta
    10681 taatattatt gttattctgt ttataaaata aaatttgata aataaaagcg atacttaggg
    10741 aggattaaaa tgtcattaaa tatagtatta gtagaaccag aaatacctca aaatacagga
    10801 aatataataa gaacctgtgc agcaacaggt tcaactttac atttagtaaa accactgggt
    10861 ttttcattgg atgataaaca cctaaaaaga tgtgggcttg actattggga tatagctgat
    10921 atacaatatt acgatagctt tgaagaatta caagaaaagt atccaaatag taaatacttt
    10981 ttttctacaa caaaggctaa acagtcacat tcagatgtga aatatgaaga aaattgtttt
    11041 atagtatttg ggaaagaaac aaagggatta ccagaacctt tgttgaaagc aaatatggat
    11101 acttgtatta gagtgcctat gttggatgtt gaaaaggcta ggtcattaaa tttatcaaat
    11161 tcagttgcta ttgtagtgta cgaggcttta agacaaatag gatacccaaa tatgaggtag
    11221 tttcaacaag cctaaatgag aaaggagcat acttaatgaa aaatctaaag ataatctgtg
    11281 acagtttatc ggacgtgcca aaagatttgt tagataaata tgatatagat atagtttcat
    11341 taacagttat actggaggaa agagaataca aggatagaat tgatatatct ggagatgaat
    11401 tttataaaag gcttagagaa gaaaatgtat atccaaaaac ttctcaagct acttatgccc
    11461 aatttaagga aatttttgag aaatatacaa atgaggatag agagatactt tatatagcag
    11521 ggtctgccac agctacaggg acgtatcaaa gtgctgtaat ggcaaagaat gatgtggatg
    11581 gtaaaatata tacatatgac tctaacttgc tttgtatggg aactggttta cttgtaataa
    11641 aagcaggaga acttgcaagt gaaggaaagt ctgttgatga aatattacct gttttggatg
    11701 aattaaaaga gaaggctttt gttatatttt cagtagatac attggaatat ttacaaaagg
    11761 gaggtagaat atcatctact aaggcagcaa taggaagtat attaaatata aaaccgattc
    11821 ttgaaataag agatggacta gtttcgcaaa tatctcaagt aagaggaaag aagaatgtaa
    11881 tttctaagat gatggaattg ataaaatcaa attgtgggga tgatttatca gaccaaattg
    11941 tgtatatagg atatagtgat gattttaagg aaaaagaaca attatctaag gttatgcaag
    12001 atgaattaag acctaaagaa attagatttt tccaaattgg tgtgtgtata ggtgcgcatg
    12061 ctggtcctgg tgtcactggt atcatatgct taaaaaataa ataatatatt tttggatgac
    12121 attatttatt tttttattaa attaggatag aatattaatt attgagatgt taaattggat
    12181 ttattttgga tgaaattaag tgttgatata ttagatttag tttgtaggga agtgtgtaaa
    12241 tagataagaa tctattgcac acttttttat agtaagattt attaaaaaaa tttcaaaatg
    12301 tacttgtaaa ttgtatataa aaaaatt
//
LOCUS       DAGQWR010000124        12024 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.124, whole genome shotgun sequence.
ACCESSION   DAGQWR010000124 DAGQWR010000000
VERSION     DAGQWR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.124"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(175..339)
                     /locus_tag="MIW95_002998"
     CDS             complement(175..339)
                     /locus_tag="MIW95_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152306.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(881..1213)
                     /locus_tag="MIW95_002999"
     CDS             complement(881..1213)
                     /locus_tag="MIW95_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBY3152307.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(1457..2098)
                     /locus_tag="MIW95_003000"
     CDS             complement(1457..2098)
                     /locus_tag="MIW95_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBY3152308.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVILQI
                     LMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKQNYSVGIILPWTLDDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(2079..2372)
                     /locus_tag="MIW95_003001"
     CDS             complement(2079..2372)
                     /locus_tag="MIW95_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBY3152309.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            2766..2825
                     /locus_tag="MIW95_003002"
     CDS             2766..2825
                     /locus_tag="MIW95_003002"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152310.1"
                     /translation="MIEYIYYYNNKRIKKINLA"
     gene            complement(3341..4009)
                     /locus_tag="MIW95_003003"
     CDS             complement(3341..4009)
                     /locus_tag="MIW95_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBY3152311.1"
                     /translation="MPVLMAIGVPVHLAIGTNKFAASAGCISSAYRYAKSGKINNDLL
                     KKLVPFTIIGSVLGVRCVLSISEEILNVLVVVMILIVAIYTFISKNLGQEDNFEAVNK
                     KNLRLGMLMALIMGFYDGFFGPGTGTFLTFGFIKIYGYDFLHASANTKILNLTSNITS
                     LLLFMINGQVDYKIAIVFALVMIMGAYVGAKVAIKKGSKMIKPIFLVMALFMVVKLVY
                     QTLV"
     gene            complement(3954..4094)
                     /locus_tag="MIW95_003004"
     CDS             complement(3954..4094)
                     /locus_tag="MIW95_003004"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152312.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGTYKHACSYGNWSASTSCHW
                     YK"
     gene            complement(4153..5808)
                     /locus_tag="MIW95_003005"
     CDS             complement(4153..5808)
                     /locus_tag="MIW95_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3152313.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQMIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKGI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVDSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(6037..6405)
                     /locus_tag="MIW95_003006"
     CDS             complement(6037..6405)
                     /locus_tag="MIW95_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY3152314.1"
                     /translation="MKYSITDVSKITKISSSAIRFYEEKGLLLPINRKPSGVRYFSEE
                     DVERLIFIKELKNTGLSIKDIHECFHFCDMGDGTIDKRIQLFQQHKQNIKNKIHELES
                     CMDKIDEKIYYLKEKKMCKK"
     gene            6554..7348
                     /locus_tag="MIW95_003007"
     CDS             6554..7348
                     /locus_tag="MIW95_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HBY3152315.1"
                     /translation="MEKYATMIKEKLNEGKVLCGVAVSLSDSVVSELIGISGYDFIWI
                     EGEHSALDRKDIQQHMIAAHAGGAASLVRLVNSDPDLVKPIMDMGTDIICFPFINTVE
                     DAQKAVAACTYPPNGIRGYNPQRASYYGNMNYFDYVKNANNETLKMMLIEQKAGYDNL
                     EEIVKVEGIDIIALGPGDLSLDMGFSGYMNRIEIKDMISHAAEICKKYKMPFIIFPTI
                     EKKSIEEWVNIGANMLCFAQDTTFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(7501..7929)
                     /locus_tag="MIW95_003008"
     CDS             complement(7501..7929)
                     /locus_tag="MIW95_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBY3152316.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(8405..9343)
                     /locus_tag="MIW95_003009"
     CDS             complement(8405..9343)
                     /locus_tag="MIW95_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBY3152317.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
     gene            complement(9687..11342)
                     /gene="ilvD"
                     /locus_tag="MIW95_003010"
     CDS             complement(9687..11342)
                     /gene="ilvD"
                     /locus_tag="MIW95_003010"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBY3152318.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIEFNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            complement(11666..11965)
                     /locus_tag="MIW95_003011"
     CDS             complement(11666..11965)
                     /locus_tag="MIW95_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152319.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
BASE COUNT         4131 a         2007 c         1166 g         4720 t
ORIGIN      
        1 ctgaatcata atctctacta aactaattac aagtttaaat tataaagact tatttctata
       61 taaatattac tctataacaa taagtttctt atttaaattt taaaatatta tctaaaaatc
      121 ataaaaaaga aggtataaag agctaccctc aagctcaata taccttataa atatctaaac
      181 ttcatttttt actaattttt ctatatcttt gataatatgt tctttagatt tttttccagc
      241 tatggcatca aaaattgcaa cagccttttt ctctccaaac ttttctgcta catcattaaa
      301 gtctaaaaat ctaacacttt cttttggttt accaaacata taacacctct catacatttt
      361 tatatattta ttataacttc tatagactta tttttcacca ataaacgtta gacaaattag
      421 tccctttatt tttttaaact agctttacct tcaaaattct taacaaaaac agtttattct
      481 actctataaa taaaaataga caatttaatt tgtaggaaac tgataataga atacagtaaa
      541 taaaactttg tttaatctat ctgaaaaagt tatctattaa aactgctatg aatttataaa
      601 gaatattcta gttcataaaa atacaaatat gtaaatataa tcaccttaat attcaattta
      661 tttaaaataa aaaatgtata aaagatttaa taatgacaat atactattct caatacaatc
      721 tatgatgtaa ataatttaaa actactatat tatttaaaaa tttatattta tatatgcaat
      781 tctaaaaact taataagaag tgaacttaga aaataaagct taatttcaaa gacttaaata
      841 gaatattatt attagtctaa ataaactacg tgtatcaaaa ttatatatta aattcttcta
      901 aaatttttat tgcaaatctt gcgtttggat atgtaataga cccgccacca attacaccta
      961 tttttatagc ttctataatt tcatctttac ttgctccaga attaatacaa ccctcaatat
     1021 gataacaaat acactcatca catcggctta aaattgaaat agctagtccc attaattcct
     1081 tatttttttt acttatagcc ccttctgaat aagtcttatc atcaagactt ccaaatactc
     1141 taaaaaactc atctgtttca cttaattttt tattgtaaaa tcccctatat tctttcattt
     1201 ctctagtatt catcttctac ctcctatgtt tttcttataa gagtatcaca ctgtacttat
     1261 attttcaata ttaaatacaa attttcattt tatgttcatt agcctaattt atattatttt
     1321 ttgaaatctc aataagttat catataagta agattttttc agaactaaaa tagaaatctt
     1381 agtaatttct ttattatcta tctcagttct gaaatatatt attcttatcc aaacattatt
     1441 atagtgatta attatattag ttatttttac ctgaaatttt atacatcaaa taagaatata
     1501 taaatggtag tattacaatt acaaagatta caaagatatt ataaaaagga atattaataa
     1561 atgcattaat aattaataat attccaccaa taagccaaat ccatccagct aaatggtgtg
     1621 tcttattcca attatcttca tcatcaagag tccatggcaa aattattcct actgagtaat
     1681 tttgtttaca tttaggaaga tagtttccaa ataaaataac tattattcct aaaattaatc
     1741 ctgtataaaa attagactcc aaactaactc ctgttgaata taaaaccatc agtatttgta
     1801 gaattactga aagtatagca actccccatt ttgataaatt ttgtataaca tttgaaaaat
     1861 tagctttttt aggctcattc tcaagtctaa accatgtata caaatggata catagcatta
     1921 acaatggtac accaaaagct gctataacct ttggagcata attatctgga tttccataat
     1981 tatcaaaatg aattgcaact tcatttggta atctattata aaaatatatt gatacaataa
     2041 atggtataaa acatattaaa gtagataaaa taaattgttt atttatcgct tttttcatta
     2101 tctttttctc ccttctccat aaattgtgaa aaccaaagca taacctcttc gaaaactgaa
     2161 atatttattt cgtagaaaat aaagttctta tactttgttt caaatatgag tcctgccttt
     2221 tttagttgag atagatgata agaaattgta gcattcgtca tattaaattt ttcactaatt
     2281 tctcctgctg acattttatt ctccctcaac ataaccaata tttctcttct tatagggtct
     2341 gaaagagctt tgaatgtttc tggaaacccc aacaccatca cctcgctatt tagaatttca
     2401 tctaaataga atataacata acatccattt tatttcaata actatttaga aaaatttcta
     2461 aatagttatt tccaaatatt tattgaaaat tttcatatta agattctcaa tttacttaag
     2521 tcgcttttat ataatcttat tttatctaat ttttttaatt taacaaaagt gtattgcacc
     2581 acaattgtta aagcaaaaac ctaatgactg atatacttat atatattaca tatactttta
     2641 atttcaaatt aaaagtagct aaatccaatt taaaaagtag ttaagtccta tttaaataga
     2701 aaagaatgaa ataagaaata tactgtataa aaatgcataa aaacctctac taattagata
     2761 caactataat agaatatatt tattattata ataacaaaag aattaaaaaa attaatttag
     2821 cataaccttg ttaaatatag atttgaatat gaatttaaag ttatgtaaaa ttaaaaatac
     2881 aggaatgaaa attccattcc tgtataaaat ctaacttttg agtctcaata caatcttatt
     2941 ttgagacaat cttttttgtt ttatattttc ttatatttaa aatataacgc atatcttatg
     3001 caacatattc atatttttct ttatattctt tcatcgcatc attccaagcg tcttgtactg
     3061 ttacacctct tttattagca attttttgtg ccagttcaat tatcttcata gctcttcttg
     3121 atatgttcat aaacattcct cctacaaatt accatatatt tcatatatat aaatcaaggt
     3181 tatattttta tgatatataa tccttctatt gtaatattac aatttatatg tgtattatat
     3241 atgaactata ttagaagata attagaattt ttataaagct atatgattag attaaatata
     3301 aaagctttac aattacttat aaatttaaaa agtctataaa ctaaactaat gtttgatata
     3361 ccaattttac gaccataaat aatgccatta ctaaaaatat tggttttatc atctttgaac
     3421 ctttctttat agcaacttta gcacctacat aagctcccat tatcatcact aatgcaaaca
     3481 ctatagcaat tttataatct acttgaccat ttatcatgaa taataacaat gaagttatat
     3541 tacttgttaa gtttaatatt tttgtatttg cagaagcatg taaaaaatca tatccatata
     3601 tttttataaa tccaaaagtc aaaaaagttc ctgtacctgg accaaaaaaa ccatcataaa
     3661 aacccattat aagagccata agcatcccta gtcttaaatt tttcttatta acagcttcaa
     3721 aattatcttc ttgacctaaa tttttagaaa taaaggtgta aatagccact attaaaatca
     3781 taaccacaac caaaacattt aatatctctt cacttattga taatacacat ctcacaccta
     3841 gaacactacc aataatagta aatggtacta atttttttaa aagatcgtta ttaatttttc
     3901 cagactttgc atatctatag gcactactta tacatcctgc tgatgctgca aatttatttg
     3961 taccaatggc aagatgtact ggcactccaa ttgccataag aacaggcatg cttataagtc
     4021 cctcctcctc ctgctataga atctacaaaa gcagctaaaa acccccctat acataaaaaa
     4081 ataactgaaa acataaaatt cctccatcat tctctttcaa tttttcttac aatatatatt
     4141 atataatctt atttaatact tattatccag catattcttt ctatattcat ttggagttat
     4201 actattaaac ttcttaaata tttgtgaaaa ataactactg ttattaaaac ctactttctt
     4261 acaaatatca ctaatactca tgcttctatc taacagatat tttttagatt cttctatcct
     4321 aagaagattt atatactcag ttattgaaac tttatttctt tcattaaaaa tagaactcaa
     4381 ataatttgga cttataaaaa aaacatctga tatactattc aaatttatat tttctttata
     4441 atattttttt atatattcgt ttatattttc aattagattt cctttatctt ttctccaaaa
     4501 tgctctattc ttatcaatac actctttgga aaattcaaat aatacattcc ataaatcttc
     4561 atcactgtct acaagtgata tttttttatt atatttattt cttaaataag atataagttt
     4621 taaatcctct aaatctctaa gcactgttct agacaataca aatatcattt cctgaatagt
     4681 atctctaaca tcattatatc ttaatttgtt tgtatctatt agattataaa caccttctaa
     4741 attcatattt ttaatttctt tttcaatata aagttctaat tcaaagggat attcttttga
     4801 agaattattg aacatatcta tcggattaat aatatactct tcttctaata atctactatt
     4861 tgctttttta agtgcctctt caagtcttat attttctttt ctactcttta atatctcctc
     4921 atcttttata ctaagttgat tttcaaaaaa attcctctta gctatttcct gaataaattc
     4981 agacatcatt aataaatttt cttttaatac atataatcta cttttaggat ttaatggaaa
     5041 ttcattgtaa attttgttta tgtattttat atcaatacca gaactacttg ataattcaga
     5101 taaattttta tctatatcaa tagacttata tactataaaa ttaccacatt ctatatatcc
     5161 aactatattt tcttctaata ctataggtat ctctaaactg gaaacaccat actcacaatg
     5221 gtacactttt tcaaagttat cattatttat gcttttaaca agttttttag tacacggttt
     5281 tatattttta caaatatcgc aaaatttatt ttctttctta gtctcaaata ttcctttata
     5341 ctttaagtca tatacagata ttctaagctt caatatatta gatatttgat ttgcaagatt
     5401 ttcaattatg tttacattaa ctaactcttg tatattgttt tcagaaaata ctataggtat
     5461 ttgtctcaaa ctcgaaaatt tatgaggaac ataagtaatt aatattacta aatcaatttt
     5521 accagtgttt ataatttcat gttctgaata tggtgggcaa taaactatag atgtttcctt
     5581 tatattgacc tttttaccat caatcatttg tattccttct ccctgaatca catataaaag
     5641 ctgttcttca cttaaatgaa aatgcttttc ttggcagttt cccggaaaaa atcttacaat
     5701 tccagcacta agtctattga attgtgtttt tgatggttcg tgtatccaca taacctctcc
     5761 ccaatcaaat ttttgtactt cattatttga ctcaataaaa tccaccataa actcacctcg
     5821 tagtttttat ctaaatattt ttttattcct ttaaatatct ccttataata tacattatca
     5881 taattttctt ttttattgta tagataaata gttatataga acattgtcca attcttccta
     5941 aaaatatagt gtaaaaaatc tttcctcaaa tgtttccaat tgaatttact ctttatatta
     6001 gaaaaatcaa aaaattattt ctttattcta taaggattat tttttacaca tctttttttc
     6061 ttttaagtaa tatattttct catctatttt atccatacaa ctttctagtt catgtatttt
     6121 atttttaatg ttttgcttat gttgttgaaa taattggatt cttttatcta ttgtaccatc
     6181 tcccatatca caaaaatgaa agcattcatg gatatcttta atagataaac cagtattttt
     6241 taattctttg ataaatatta gacgctctac atcttcttca gagaaatagc gaacaccact
     6301 tggttttcta ttgataggaa gtaataaacc tttttcttca taaaatcgaa tagcagatga
     6361 agaaattttt gttatttttg atacatcagt tatagaatat ttcataatct ctacctccct
     6421 aaattttata tgaataagaa tattataaaa ataccttata cagtagtcaa caaaaaagta
     6481 ttgacttcaa gttaggttga agttgtactc tttgaagtgt aaaagaaaac ttacaaaaga
     6541 aaggatgata taaatggaaa aatatgcaac tatgattaaa gaaaagctaa atgaaggaaa
     6601 ggtcttatgt ggagtagctg tttcactgtc tgattcagtt gtaagtgagt taatcggtat
     6661 atctggatat gattttattt ggattgaagg agaacatagt gctttagacc gtaaagacat
     6721 tcagcaacat atgatagctg cccatgctgg tggtgctgct tctttggtac gattagtaaa
     6781 cagtgaccct gaccttgtta agcccataat ggatatggga acagatatta tttgtttccc
     6841 atttattaat acagtagaag atgcacaaaa agccgtagca gcttgtactt atcctccaaa
     6901 cggtataaga ggatataatc ctcaaagagc ctcttattat gggaatatga attattttga
     6961 ctatgtcaaa aacgcaaaca atgaaacatt aaaaatgatg ttaattgaac agaaagcagg
     7021 ttatgataat cttgaagaaa tagtaaaagt cgagggtatt gatattatag cattaggccc
     7081 tggtgactta tctttagaca tgggtttttc cggatatatg aatagaattg aaataaaaga
     7141 tatgatttct catgctgctg aaatatgcaa aaaatataag atgcccttta ttatttttcc
     7201 tacaatagaa aagaaaagta ttgaagaatg ggttaatatt ggagcaaata tgctttgttt
     7261 tgcacaagat actaccttta tcacttatgt tatcaatcaa ttattatctt tttataatga
     7321 gattgtacca attcaaaaac aacattaaat tataaatcta attttatatt aaaagagact
     7381 aatattttat ctatctctat taaggatatt ttcttatgta tcataatatt agtctctctt
     7441 attattatat actgctatca tcattagttg ctatttatac tatttttata atatacaaat
     7501 ttattcagct aacgttttgc ctaattctat acactcatct tttgcttctt catttgggtc
     7561 ttccattgct gtaattcctt ctccaccaat aattgtagca ccagcatttt gcatacgttc
     7621 ttcccagtct ctcatccatt cacaatttcc ccatccatat gaaccaaata atccaatctg
     7681 tttacctgat accatagatt ctaattctga aacaaatggc tccatatacc cctcttctag
     7741 ttgctctgct cccatagatg ggcaacctaa tgcaaattta tcttcttctt ttaaatcact
     7801 tggtttcagt aagctcacat ctaaaacttc tggtgtctta ccttttaact tcacaccttc
     7861 agccacaaaa tttgccattt tttcagtatt tcctgttcca ctccaataaa caatatatat
     7921 tttactcatt tttacctcct gtagtatttt ttaattaatc aatagttagt tatagctaac
     7981 taacatctaa aaaacaaaag atttctttat ctgaaatctc tttatttttc taaatataga
     8041 atatcacaat tgattttgaa attcaatatt tattgataat atttatcatt tatattttat
     8101 attcatttta taatatcaaa acactgtatc gtatttctaa tctttcatag aaaaatttct
     8161 aacttatagt acttttgcat ctcttataaa tcttttatat gtataagtac atagaaaaac
     8221 acaccttttc cagatgtgct atctataata tcgtatattt atttttcaat tctaactagc
     8281 taatatgttt ataaatttta tcacttaata cctttataca ttgatgtaaa atcttacacc
     8341 taagtaattt ttattatttg tctcgactct atataatata gctattttat catcaaatca
     8401 aaaattatct tttgctatta ttcttgtatt tataaagatt taaatataag tatccatttc
     8461 cttgttccgt tctaggtatt ccaagcgctc tcgtacattt tattaattgt gcatacagtt
     8521 cagcttcatc taaatctctt tcaaactcct ctcttgccca ttctttaatt agtgctaatg
     8581 agcctgatac gtatggtgca ctcatactag taccactcat aatagcaagt ttattatcca
     8641 tataagtcga tataatattt cttcctggag ccaccaaatc tattgtagta tttgaattac
     8701 taaacttttc aaccaaatag ttttcattta ttgcacctac ttctattacc tcagcataac
     8761 tggctggata actatactca cttgtactag aatcaccatc tccattatta cctgctgcac
     8821 acactacaga aatattattt tttactgctt gcatgacagc attttttaaa ttcttatcat
     8881 ctttgtttcc cccaagagac atagatataa tatcaacctt gttatttaca gcaaagttaa
     8941 tagcattaat tatactttga tatgttccgg taccatcttt atttaatgct tttgctatta
     9001 ataatttaca atctggagct actcccataa cttcattatt atagttagat gcagctataa
     9061 tacccgccac atgagttcca tggccattaa aatcctcata tatattttta tttccattac
     9121 tgtcatcact aaaatttgca ccaccaatta tttttccttt taaaagagga tgagatatat
     9181 cacaacctgt atctattata ccaactacaa tatttttacc agtataacct tcatcccaca
     9241 ttcccctagc attcatttgt tttattccat atgggaattt attttttgca cctcttagat
     9301 tttcatttat ttcatacgga attagtttca ttcttttatt catcatatat cctcctaaaa
     9361 attttattgc ttataaaaat catgaatttt attttataat aggtttataa atcaaattaa
     9421 aacctatatc tacttattta tatttatgat atatttttta tttttaggag tcatattttt
     9481 ttgtatataa aataatattt agataactat attaaaaaat acatgtttgc ttatatacaa
     9541 aacttactaa aatatcttta atatttttgt atacaaacag tatatttttc atacaaaata
     9601 aaaaggatac ctcattagtg ctataacacc taagatatcc aattaatatt ttcttaattt
     9661 tttctaattt tcgtaaatat agttcactat tttaaaactg ctcctgtatt tgctgatgtt
     9721 accaatttag catatctact taagtatcca tgctttattt tcggttctaa aggtttaaat
     9781 tttaattttc tattttctat ttcaacttca tctactttta actctaattt tttgtctggt
     9841 atgtttatag aaattatatc tccttcttca actagtccga ttaaaccacc ttccatagct
     9901 tctggagaaa tatggcctat agatgctcct ctagttgctc ctgaaaaacg accatcagta
     9961 agaagtgcta catgcttatc aagtcccatt cctgcaactg ctgatgtagg agatagcatt
    10021 tctttcattc ctggaccacc ctttggacct tcatatctta taactataac atcacctttg
    10081 tttatttttt taccaaaaat agcatttaca gcttcttctt ctgaattaaa aactcttgct
    10141 ggtccttcat gaactaacat ttcttctgca actgctgact tttttacaac agctccattt
    10201 agagcaagat ttcctttcaa tattgcaagc ccaccttggt tactatatgg attctttaat
    10261 gtatgtatta cttcttcatt ttctatttca caatttctta tattttcccc tatagtcttg
    10321 cctgttacgg ttttgcaatc taaatttatt ccatttatct ttgaaagttc gttcattata
    10381 gctggtattc ctcctgccat atgtaaatct tcaatatgat gttttccact tggacttaat
    10441 tttgttaaac aaggagtttt ttcacttatt tcatcaaaaa aatctaagtt aaactctatt
    10501 cctgattcat aagctattgc aggtaagtga agtactgtat ttgtagaacc agccattgcc
    10561 atatcaacag ctatagcatt tttaaaagca tctattgtta aaatatctct aggttttatg
    10621 tcgttcttaa gtaactccat aacatacata cctgcatatt ttgctattct ttttctttct
    10681 ccagaatgtg acgcagcagt accattatat ggtataccca tgcctaaagc ttctgttaaa
    10741 caattcatgc tatttgctgt gaaaagtcca gagcatgaac cacatccagg acaagagttc
    10801 atggctaact cttctaattc ttcttcactt actgttccat ctttacatgc tccaacaccc
    10861 tccattgcag agttaaaatc atatactttt ccattttttt tacctggaag cataggccct
    10921 ccactcacaa caatacttgg tatattaagt cttgcagcag ccattaacat acctggcact
    10981 atcttgtcac aattaggtat aagtaccaaa gcatcaaaac catgtgccat agccatagct
    11041 tctattgaat ctgctattaa ttctcttgaa gccagggaat aattcattcc gacatgcccc
    11101 atagctatac catcacatac ccctatagca ggaaattcta atggcaaacc accactcatt
    11161 cttactccat tttttgcagc ttctgctatt tcatcaagat gcagatgacc aggaatcacc
    11221 tcattttgtg catttactat acctattaat ggtctttcaa tttcttcttt agtaagaccc
    11281 attccataca ttaacgctct ctttggagct ccttctattc ctttttttat atcacttctc
    11341 ataatattac ctctttctta cttttttaaa ttttcataat attattataa tatatttttc
    11401 tattaattaa ttacgaaaat tgtaaaaaaa tattagcata tactaatgaa aatttattac
    11461 gattttttat caatatctta cacaatcgta ttatataata ttatcttttc aatatatttt
    11521 ataaactaat atattttaca aattgttgag taatatttta tgatataaat cagaagaagc
    11581 acttctataa tagaaagtgc tcaactatga ataagattaa taatatattt gccttagata
    11641 tatagacttt tataaatatt aaagattaat caaatctgat gtttgaaact ttatcccaat
    11701 taatcttcct tgaatagtct tctggatgtg ataatctaac tatatgagtt gaattatcat
    11761 agctgtaata aattgtagta gtaccatcaa ctggattctt tttgtatcct tgatatacag
    11821 cactatttgg tactttcttt tcaacaaagc tatatgcaga tgaagtatca atagtttcta
    11881 ttttattagg aaatataaac atataaaaag ctactactat aaaaatacat atgcatgtaa
    11941 tactatacca tttaatccat ttcataaaat tcctcctact ttaccaaaat atactattat
    12001 tatttccata tatttataat attt
//
LOCUS       DAGQWR010000125        11940 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.125, whole genome shotgun sequence.
ACCESSION   DAGQWR010000125 DAGQWR010000000
VERSION     DAGQWR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11940
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.125"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            121..1407
                     /locus_tag="MIW95_003012"
     CDS             121..1407
                     /locus_tag="MIW95_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBY3152320.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            1493..2800
                     /gene="grdB"
                     /locus_tag="MIW95_003013"
     CDS             1493..2800
                     /gene="grdB"
                     /locus_tag="MIW95_003013"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2540..2542,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBY3152321.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVVTGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            complement(3016..3240)
                     /locus_tag="MIW95_003014"
     CDS             complement(3016..3240)
                     /locus_tag="MIW95_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBY3152322.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(3337..4590)
                     /locus_tag="MIW95_003015"
     CDS             complement(3337..4590)
                     /locus_tag="MIW95_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBY3152323.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIGLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            4850..5839
                     /locus_tag="MIW95_003016"
     CDS             4850..5839
                     /locus_tag="MIW95_003016"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBY3152324.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEVHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            5911..6651
                     /locus_tag="MIW95_003017"
     CDS             5911..6651
                     /locus_tag="MIW95_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBY3152325.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            6851..8002
                     /locus_tag="MIW95_003018"
     CDS             6851..8002
                     /locus_tag="MIW95_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBY3152326.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLEPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            8027..8782
                     /locus_tag="MIW95_003019"
     CDS             8027..8782
                     /locus_tag="MIW95_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBY3152327.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKNTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            8974..11361
                     /locus_tag="MIW95_003020"
     CDS             8974..11361
                     /locus_tag="MIW95_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBY3152328.1"
                     /translation="MKKFTSKKVARFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SDTYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSVKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMDGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVSYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFNIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(11592..11825)
                     /locus_tag="MIW95_003021"
     CDS             complement(11592..11825)
                     /locus_tag="MIW95_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBY3152329.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT         4748 a         1313 c         2146 g         3733 t
ORIGIN      
        1 tctatgacat taatacatag aatatatatt ttaaataatt ggcatagaaa atgctttata
       61 tattaataaa cgtaataaat atttttaatt gcgattaaat atattaaggg ggattttcat
      121 atgaagctag agctagggaa tatatttata aaaaatgttg aatttggaga aaagacagaa
      181 gtaaaagatg gagtactata tattagtagc gaagaaatag aaaagatagt attagaagat
      241 gaaaggattg taagtgtaaa tgttgaactt gcaagacctg gcgaatcaat aagaatagct
      301 ccagttaaag atgtaataga gcctagagtt aaaattggag atgaaagcaa aatatttcct
      361 ggaattataa ataaagtgaa gactgtagga agtggaagaa cccatgtact tttaggagct
      421 tgtgttgtaa cttgtggaaa tatagttgga tttcaagaag gcgtaattga tatgagtggt
      481 cctactgcta aatatacacc tttttcaaaa acaaataata tatgtattgt tataaaagca
      541 aaggatggta tagatactca tgattatgaa gaagcagcta gaattgcagg attaaaaata
      601 ggtgcatatg taggtgaggc aggaagagaa gttgagccag atgaagtcat tgtatatgag
      661 acaaagccat tattaaaaca agtaaaagaa tatccagacc tgccaacagt agcatatgtt
      721 cacatgttac aatcacaagg acttttacat gacacttatt actatggggt ggatgctaaa
      781 cagatagtgc ctacatttat gtatccaact gaaataatgg atggagctat aatatctgga
      841 aactgtgtag caccatgtga taaggttaca acattccatc atttaaataa tccagttata
      901 catgacttat ataaaagaca tggaaaagat ttaaacttta taggagttat tttgactaat
      961 gagaacgtat tcttagtcga taaagagaga agctctgata tggtagctaa attaatagaa
     1021 tttttaggag ttgatggagt tctagtgaca gaagaaggtt atgggaatcc agatactgac
     1081 ctaatgatga attgtagaaa atgtagtgaa gttggagcaa atgtagtttt gataacggat
     1141 gaatttcctg gaaaagatgg caagagtcaa tctatagcag acgctactaa ggaagcagat
     1201 gcagttgttt cttgtggtca aggaaatcta gtggttcatt tcccagcaat ggaaaaaatt
     1261 ataggaactt tagattatgt tgaaatgatg ataggtgggt ataaaggatg cttaaatgaa
     1321 gacggaagca tggatgcaga gctacaaatt ataatagcat caactatagc aaatggctat
     1381 aatcatttga cagcaagata ttattaaata agtatttgaa tataaagttc tgttgatgta
     1441 atcgttggtc taattgatgt aataactata aagtgatttg gaggattaag agatgagtaa
     1501 aatcagagtg gttcattata taaacaattt ctttgctggt ataggtggag aggaaaaagc
     1561 agatattccg ccagaaaaaa gggctggtgt agtaggacct ggaatagcat ttcagagtca
     1621 atttaatgat gatgcagaga taatctcaac tgtaatttgt ggagatacgt attttggtga
     1681 aaatattgag agtgcaactc aagaactttt aaaaatgata aaagaagaaa gccccgattt
     1741 atttattgca ggcccagcat ttaatgcagg tagatatgga gtagcttgtg ggactatttg
     1801 taaggcagtt gaagaagagt taaatattcc tgtagttaca ggaatgtata aagaaaatcc
     1861 aggtgtagat atgtttaaat tagatttgca tatagtgtct acaggaaatt cagcagcaag
     1921 tcttagaaag gtagttccaa taatgacgaa actaggtctt aaattggtta gaggtgagga
     1981 aataggacct cctgaagagg aaggatatat aatgagagga ataagaaaaa atttcttcca
     2041 tgaacttaga gggtctgaaa gagcaattga tatgttggtt aaaaagatga aaggggaaag
     2101 ctttgaaact gagtatccaa tgcctgaatt tgatagagtt acacctatga aacctgttaa
     2161 agatttatct aagataaaac ttgccctagt aacttctgga gggatagttc caattgataa
     2221 tccagacaaa attgaatctt caagtgctac taaatatgga atttacgatt taacagatat
     2281 ggattctatg tcaaataaag aatttacaac tatacatggg gggtatgaca gagcatatgt
     2341 ccttgaaaat ccaaatttag ttgtaccatt agatgtagtt agagagcttg aaaaagaagg
     2401 tgtaattgga gaattagtag attattttat aactactaca ggaacaggaa cgagtgttgg
     2461 caattctaag agatttggag aagagttttc taagaaattg ttagaagatg atgtagatgc
     2521 tgtaatttta acttctacat gaggtacttg tactcgttgt ggagcaacga tggtaaaaga
     2581 gattgagaga gctggtatag cagttgttca tatatgtaca gtagtaccaa tatcacttac
     2641 aataggagct aatagaattg ttccagcagt agggatacca tatccacttg gaaatcctat
     2701 gttaggagaa gaggaaagta aaaaaattag aaagaatata gttttaaaag ctttaaatgc
     2761 actaacagag gatatagaag aacaaacagt atttgaataa taaaaatata atatagaaat
     2821 aaaaaaagat atcataaaat agtttttgaa aattatttta tgatatcttt ttgctatgga
     2881 tggaatgaag aaagcaatta agtaataaaa atatttatag taaaacagcc aattaaagta
     2941 taaagaatga aaaaaataaa tagaagatta tacataaaca aaaaaagaga ttttaaaatc
     3001 tctttttatt atatattaat tattttttaa atttatattt tttgcatcag attttctaat
     3061 agctaagtca aaccctctaa atctaatcac tatagggtca ttaagaggag ctatcttttt
     3121 cacttcaact tcagtgccat ctattagtcc taaggctaga agtctcttca taagtttttc
     3181 atttccagct atattgtcta taattccctt ttgtccaagt tttaagttgt aaacagtcat
     3241 tattaacacc tcctaatgaa aatcattatc aaactttata tttataatat ataacaaaaa
     3301 gagtaaattg tcaatatata acaaaaaata aacatactaa gttagtatgt ttataaaaaa
     3361 tagtatacat gggatactaa tagcatctag caaaaaacca cttactaatg gtacaaccaa
     3421 aaaggctttt ggagaatcac catatttttg tgtcaaagaa ctcatatttg ccaaagcgtt
     3481 cggagtagct cctattccat gtccaatcaa accacttatt atgatggcag aatcaaaatc
     3541 ttttccaagt actctaaagc aaataaatat tgaaaatagg attataaaga ttacttgaaa
     3601 agttactatt aagagaatag gaggtaataa tgtagataat tcaaataaat ctatattcat
     3661 aagtgataaa gttaaaaaaa ttcccaatga aagttctttt aaaaaatcta taagtttaaa
     3721 atttaattct aatatattta tcttatcatt taaatttcta aaaataacag ctgaaaacat
     3781 acaagttaca acagagggta atagtatatt aaacgttata tagaagcatc tacttataat
     3841 ttctcctgta tttatacaaa taaatatgag aagaacctgt tccaaaaata cagatggtgt
     3901 tattgtttcg gagaagttat tattttttat cctattctta tttctaaaaa atttacttcc
     3961 aaatctcttt agatttctat tatatctagg aatcgacaag cctatatttg atgatggttt
     4021 taatttatat ttatttatta gaaatttagc tacaggacat cccaatatac cacccataat
     4081 aagtcctatt gttgcagcag ataatccaac cccaactgct ccttctatac cgagattttc
     4141 tattgtaagt ccaaatgcag cagaataacc atgaccacct tccatagata catttccaca
     4201 cataagccct aaaagaggtt caagttttat aatctttgat attgtaatag ctaatatatt
     4261 ttgacaaaat gccaatactc cacacaaaat ccaatatcta aataatacac taccaccttt
     4321 ctttataaga tttatgctta tttcaagtcc tatagttgta aaaaagaagg ttataaaatt
     4381 tggcattaaa tttccactta tagatatatc tgctatatta aaatatttaa aaaataaatt
     4441 tagaaaacaa aataatagac ctcctattac tggtgcaggt atacagaaat tatttaaaaa
     4501 gttaacttta ttcttcaata aattaccaat taaaaagaag aatatagata gtgtcaaggt
     4561 ttgcactata tcaaaactta aggtaatcaa aataagtcct cctttgtaat tcaaagtttt
     4621 aattatataa cttaaattct tatgtattga aattttatta tcaaatgtat attaataaat
     4681 gtatattaaa aagaatcagt atatataatt agatagtcat aaattaaatt aaaatatttt
     4741 aatgagatgt gactttttta gaatgaaaaa ccgtagtata ttgatataat agaaataaga
     4801 cttaaaaatc tgttgatagt attatttata agtacagtag gaggataaaa tgtttgaaaa
     4861 tttacctaag atagatttac attgccactt agatggaagt gttagagtag aaaccatgct
     4921 tgatatagct attaaagaga aaatagattt accttcaaat aatatggatg aaataaaaaa
     4981 attagcaaag gtttctttta attgtacttc actagatgaa tatttagaaa aatttgattt
     5041 acccttaaaa gtaatgcaat ctaaggaaaa tttaaaaaga attacttttg aacttttgga
     5101 agatgcaagt agagaaaatg taaaatatat tgaaattaga tttgcaccat tacttcacac
     5161 ccaaaaaggt atgagtgtta aaaatataat tgagggtata attgaaggaa ttagagaagc
     5221 tgaaagtatt tatgatataa aaggcaattt aatattaggt tgtatgagaa caatgacctc
     5281 aaaagaggca ttattagtaa ttgaagaagg gaaatcattt gtaaataaag gtgttgtagc
     5341 agtagacttg tgtggtccag agaaagaggg attttgtaag gaatataaag atgtttttaa
     5401 attagcaaga gaatatggat ataaagttac aatacatgca ggagaagctg caagtggaga
     5461 aaatgtattg gatgctataa atattttaaa ggcggatagg attggtcatg gggtaaaaat
     5521 taaagaccat aaaaaggcgt ataatttagt taaagataaa aaaatattgc ttgaattatg
     5581 tccaactagt aatgtacaaa ctaagacagt agattcttat gaggtacatc cattttatac
     5641 tttttataaa gataatttac atgtaagtat aaatactgat aatagaactg tatcagatat
     5701 aaatttaagt tcagaactaa atgttatatt tgatactttt aaactaggat tagaagacta
     5761 taaaattatt tatagaaatg cagtagaagc tagttttgca gataaagaaa ctaaagagta
     5821 tttgaatagt ttaatttaat agataaaaat atttaaactt taaattaagc tattaaattg
     5881 acaaaaatac attgtattag gagaaaaaat gtgaaagatt atgaagatgt tgtttatatt
     5941 aaactagtca aaaaagaact tgaagaatgg aagaaaaata tattaaagaa accttctata
     6001 tttgacaaag catcaaaggg tatacagaat aaatttaatg gagtattgcc agataaatat
     6061 catgaaataa ttacttcttc tataaaagta atgacaaaaa ccgtactgtt tggatataaa
     6121 tatgctacaa agccaccact tagaaatatg tcattagaag aaagagaaaa attagtttta
     6181 gaaaaagcta aaacttacaa gaccacagca atgattgaag gggcaggaac aggggcagga
     6241 ggaatcctct taggtttatc tgattttcct ttgcttttag gtattaaagt gaaattttta
     6301 tatgatgtag cttcaatata tggatatgat gttaaagatt atagagaaag gatttatata
     6361 ttaaacatat tacagttggc tttttctagt caacagcata taaacaaaac ttttaaattg
     6421 atgaataact gggatgaata tgtaaatagt ctaccagaag acatcaatga ttttgattgg
     6481 agaacatttc aacaagagta tagagattat ttagatttag ctaagttttt acaactaatg
     6541 cctggaatag gagcatttgt aggattttat gtgaatggaa aattaattga taaattatgt
     6601 gaaactgctg tgtctgctta tagattgagg attcgagagc ttaagttata agagaaatta
     6661 taacataaaa aattagttat acaatatatc tatatgtatc taattttgca ttgataaaaa
     6721 caattatgga taaaaggtaa agcatgatag aatctaatgt agaggtttgt attttaatgg
     6781 aaaaatttag tttttattgc ttaagataca aattgaatta cgaagagaag taattgggag
     6841 gaatattttc atgacaaatt tgccaaaaca atttgatagc ttcaatgaag ctagacaaaa
     6901 gggatttata aatgcaaagg aacttaagga aagtgggaaa aagatggttg gagttttctg
     6961 tacatttact ccagttgaaa ttccaatggc agctggagca actgtagtag gtgtatgcgg
     7021 tgttagtgaa gagccaatac ctgatgcaga aagggtgctt ccaagaaatc tttgtccttt
     7081 aattaaatca agctatggac atgcaataac agacacctgt ccttatttct atttttctga
     7141 tttgttgata ggagaaacca cttgtgatgg taagaaaaaa atgtatgaag aacttgcaaa
     7201 agttaagcca acatatgtaa tgcatttgcc aaatacttca aaaggagaat ttgcctataa
     7261 actttggaaa gatgaaatga taagattaaa agaagaggtt gagaaatctt taggtgtaac
     7321 tataactgaa gaagatataa gaactgctat aaaagataaa aatgaagaaa gagaactttt
     7381 aaaagagttt tatgcacttg gtaaattaga accatctgca cttacaggcc ttgagttaca
     7441 taatgtttta tatcaagcag gttttaaatt tgaccgtgca gaacttaaac attctttaag
     7501 aaaagttata gatgatatga aagaaagata tgaaaaaggt gaatgtcctg ttcaaaaaga
     7561 taagccaaga atattgataa caggttctcc tattggtgga ataagcgaaa aaatagttaa
     7621 aactttagaa gatgcagggg catcagtcgt tgcatatgaa ctttgtggag ctattagatc
     7681 aaacgattta ttggttgatg aagaaataga agatgtatat gatgctttaa cacaaaaata
     7741 tataaatata ggttgttctt gtatgatgaa taatgacaat cgtattgaat tattagatag
     7801 aataatagat gaatataatg tagatgctgt aatagatgta gttttacaag catgtcatac
     7861 atttaatata gaaagttata gaataagaga atttgttaca aaagaaaaaa ataagccatt
     7921 tatgtctctt gaaactgatt attcaaaatc agatactgaa caattaagaa ctagatttga
     7981 agcgtttgta gagatgttgt aggaataaat aaaagtagga ggaactatgt ttagtatagg
     8041 agttgactca gggtcagtgg caacaaaggg tgtgctattt gatggtgaaa aaattattaa
     8101 gaaaataatt ataccaactg gatggagccc taaaaataca tctaaacagg tctatgagtt
     8161 gctaagtagt gaaattgaca aaaaagatat aaaaaaggtg gtaggtacag gttatggaag
     8221 gggagtaatg gattttgctg ataagaaagt aacagaaata acttgtcata ctagaggtat
     8281 ttacttttta aacaagaaca taaggactat tttagatgta ggtggtcaag atagtaaagt
     8341 tataaattta gatagagatg gaaatgtatt taactttata atgaatgata agtgtgctgc
     8401 aggaactggt cgatttttag agattacttc taacctttta ggaagcgata ttgaaagtat
     8461 agatacatta gctaaaggat atgaaccagt taatatatct agtatgtgta cagtttttgc
     8521 tgagtctgaa attgtaagtc ttctagctca aaacatttct actggagagg tagcagcagg
     8581 gatacttaaa tctattgcaa ataaatccac ctcaatgtta gcaagaggtg aggtaataga
     8641 tgaagttgca tttactggag ggcttgcaaa aagtaaagaa ctagtaaaaa tgatagaaga
     8701 aatactgggt aaaaaaattt ttattgcaga agatactcaa ataataggag ctttaggagc
     8761 tgctgtaata ggatttagat aaaaacatat aaaaagaaaa tgaaaagctg aatttatcat
     8821 aaataagttc agttttttat tttattttgt ttatttataa aggaaaactt tggtataatt
     8881 gatttaaatg attgtatatt tgtaaatact tgatatttag tcatatataa cattagaatt
     8941 ttaaaaatta gctcaaaata agggggagaa agcgtgaaaa aatttacttc aaaaaaagta
     9001 gcaaggtttg ttgcactgtt tttaatatct attttagtaa tgtcaacaat tcctgtgtca
     9061 gctgacaata gcaatacatt gaatcagata aaaaaagcag aatacagtga tacttataaa
     9121 caatatctaa atgatgctaa aaatggacgt acagaaaaat ataatggaat tataccaaat
     9181 ccatatcagt tagaaggtac tcaaattcaa agtaaaggaa gaacagctcc aacatcttat
     9241 gaccctagaa aattaggtct tatgacatca gtaaaaaatc aagaggattt aggaatatgc
     9301 tgggattttg ctgcaatggc aaccttggaa agtttcttga agttaaataa ttatggagac
     9361 tacgatttat cagaagaaca cttaagatgg tgggcttcag atggtgaata tggatggtct
     9421 gtaaatgata tggatggtgc tttaaattat gaagctatgg gatatttaac atcttggtca
     9481 ggtcctaaat tagaaaaaga tatcccatac aatggtagag tatctaaagc acaaggagca
     9541 aaaaaaccaa ctaatatgaa tacagcacct actgttttta atgtgacaga tgctgtgtgt
     9601 gtaagtaatg acataaattc tacaaaaaat gctatattac agtatggagc agtaacatct
     9661 ggttattatg aagatattaa atatcaaagt gatgaccaaa attcatatta ttgtccaaca
     9721 aaaagtttta atacaaatca tgctatttca attgtgggtt gggatgataa ctattctaaa
     9781 gataatttta atgcaaatat tagaccatca aaaaatggtg cttggttaat aaaaaatagc
     9841 tggggagatt ataattctga gggtggatat ttctggatat cttatgaaga caagacactt
     9901 atgtctgata tagataattt ttcaatcaaa ggtggtaaaa aaccaaatga tgatgagaag
     9961 atgtatcaac atgactatgc aagtattgta cctttaattt caaaaaagat aacagctgca
    10021 aatgtatttg atttcaatag aggcgatgaa actctaaaat ctgtaatgtt cttaactgaa
    10081 agtataggag ctaagtatga agtatcttat gctccagtag taaatggtat acctcaagaa
    10141 aacaatatga aaaaattaaa agaaggtact gcccaatatt caggatacat aacagttcca
    10201 attgattctt ttaatatacc agaaggaaag ggtgcaattg tagtcagtat agaggggaaa
    10261 aatggagagt caactatagg ttctgagtca aatgtaccag gatatgatac atttaaagca
    10321 aaagctaatt taggtgaaag ttatatcata gataatactg gaaaattctt tgatataaat
    10381 agagatagta acttctatcc gtgtaatttt actataaaag ctgttacaga aaagtcttct
    10441 ggtgagagta tcccgaatga gtctttaatt ggtagtgata gatatgaaac tgctatcaaa
    10501 gttagtcaaa atggtttcaa ttcttcagaa aatgtagttt tagtaaatgg cagttcaata
    10561 gtagatgcat tggcagcaac tccttttaca tctgctataa attcaccaat attattgaca
    10621 caaaaagaag ctttaaattc aaaaactaaa gctgagatac agagattagg tgctaagaaa
    10681 gtatacctaa taggcggaga aaattctata agtaaagaaa ttgaacaaca attaaaaagc
    10741 ttaaatatat cgatagagag aatatcagga agtgatagat acaagacaag tttattatta
    10801 gctcaaaagt taaatggtat aaaaaatgta tctcaaatag cagttgtaaa tggagtgaaa
    10861 ggattagctg atgcaattag tgtaggtgct gctgcagcag aaaataacat accaatcata
    10921 cttgcaaatg aaaagagcga attgcaagga gctgatgaat ttctaaattc attaaatata
    10981 gagaaatctt atattattgg aggaacagca tccctatcaa acaatctaga aagtaagtta
    11041 aaaaatccaa ctagattatc tggtagtagt agagacgaaa ctaattcaaa gataatagat
    11101 aatttttata aaaaagatac tttaaaaaat gcatttgtag ttaaaaatgg aatcaagaat
    11161 caaaatgatt taatagatgg attggcagtt ggtcctttag gagcaaaaac tgggtcacca
    11221 gtaattttag ttggtgataa attagctgat agccaaaaag aagttcttaa aaataagact
    11281 ttagaaaaag taactcaagt tggtggagga gcaaataaaa atgcatttaa agagttagta
    11341 aaattaaaaa gtgctaaata gagtacttaa gtattaacat atattagtgt gtattagtaa
    11401 cttatcttaa taaaagctga aaataattac tttaaattat tgataaataa gataatcaat
    11461 gattgtaaag ttttgttttc agctttaaat ttatgtagta taagtttaaa aacataaaaa
    11521 agggttacta tatatagaaa tatagaaaat cttatatttt atatataaat aacccatatt
    11581 taattaactt tttatagtgt tctaatagat attattgaac ttaatggtaa atataatgtg
    11641 taagtttcat ttaaagttgg attagattca tctatatatc ttgagcaaag tgtaattgat
    11701 aagctatctg cttctataat ttcaccatat agaggacaac tgctaaagta agaatgtact
    11761 tcaacatatt tatttaagtt ttcttgaaca taatcgtaaa aacggttttt tgtagtagta
    11821 gccataaaat caactccttt attttttatt aaagttgaaa cttttaaaaa gataaatatg
    11881 agtacttcta ttagaaacta agttaaaaaa taagcaaata gatatgaaaa ttagaataat
//
LOCUS       DAGQWR010000126        11921 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.126, whole genome shotgun sequence.
ACCESSION   DAGQWR010000126 DAGQWR010000000
VERSION     DAGQWR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11921
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.126"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(41..1327)
                     /locus_tag="MIW95_003022"
     CDS             complement(41..1327)
                     /locus_tag="MIW95_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBY3152330.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(1342..2607)
                     /locus_tag="MIW95_003023"
     CDS             complement(1342..2607)
                     /locus_tag="MIW95_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBY3152331.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            2923..4020
                     /gene="ychF"
                     /locus_tag="MIW95_003024"
     CDS             2923..4020
                     /gene="ychF"
                     /locus_tag="MIW95_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBY3152332.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            complement(4499..6778)
                     /locus_tag="MIW95_003025"
     CDS             complement(4499..6778)
                     /locus_tag="MIW95_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY3152333.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITEKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYIARSNGNIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFAQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(7049..8503)
                     /locus_tag="MIW95_003026"
     CDS             complement(7049..8503)
                     /locus_tag="MIW95_003026"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBY3152334.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYNSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            8667..9536
                     /locus_tag="MIW95_003027"
     CDS             8667..9536
                     /locus_tag="MIW95_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY3152335.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGYLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(9750..11822)
                     /locus_tag="MIW95_003028"
     CDS             complement(9750..11822)
                     /locus_tag="MIW95_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY3152336.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLSHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKLLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNCLSTISPVVFTNLEQNQKLSIKKGITTAKEENRVSIL
                     RYDIEKIG"
BASE COUNT         4077 a         1937 c         1287 g         4620 t
ORIGIN      
        1 aaataatact taattagtta ttcttattat gattaagttt ctatttaggt tctacaatag
       61 aaataacaca atactcttct ttaaaatgct cttttaatct ttcttcaact tcctcaaaag
      121 taatatcttt aataacatct aaataatcca atagatttat atccttaaaa acataagata
      181 taaaactatt acctataaag tttatagaat caaagcactt aataaatgac cctatttttt
      241 tctttttagt tctctcaaac tcttcttttg ataatccttc tttttttgat ttttctatat
      301 aatcaagaat tatttctttt acttttttag gctctttaga atctccagct attatgctaa
      361 aagcataatc cacttgagac gaaaatccag ctccaaaatt ttcatttatt aaaccttgca
      421 tatacaaatc ttcatagagc ttacttcctt ttttaaataa catccctaca agtatatcag
      481 ttacaatctc tttacgtaaa agttctttcc cttttaaacc aacattacta tctttaaaac
      541 ctatgttgaa cataggcata gatataggga atttttctat aacctctttt tcttttacac
      601 tttctggttc ttctggataa aatctttcta tactcttaga gagtttgtct actttgtaat
      661 tgtttgactt tttagttaca tctataactt tttctacatc taagtcacca acaacaaata
      721 aagccatatt tcctggatta tagaaagtat tataacattt atatagctct tccttagtta
      781 tcttatatat actatcaaca gtacctgcta tatcaattct agcaggataa tttacataca
      841 ttgccttcaa gcaattaaag tatacattcc aatcagggtc atcattgtac atttttatct
      901 cttgtgctat tatacctttt tccttttcaa cattttcatc tgtaaaataa ggtgtttgaa
      961 catagtcaat tagatgttct aaactctcat aaaaattttc agtagcagaa aataagtagg
     1021 ctgtcattgt aaaattagta aatgcatttg catttactcc taattttgaa aatttatcaa
     1081 atgcatctcc tccatcaggt tgctcaaaca tcttatgctc tagaaaatgt gcaatacctt
     1141 cattcactct aattttctta tcttctccaa ttggtacaaa ttctaagtca tttgaaccat
     1201 aattagtagc caaaatagca tactttttca taaaacccct tttaggcata aaatatacat
     1261 ctaatccatt ttgcaatttc tcataataaa cttcttcttt caatatatca ttaactattt
     1321 tttccataga catctcctcc attaatttct caagaagtat acagtatcaa gctctatatt
     1381 ttttatagct tcaactatat ctttttttgt aaccttttct atactagata ttatgtcttg
     1441 tacttcagaa tttgtctttg ccatagactg agagaaaaca aaatcagaca taccacctat
     1501 attatcagtt atagatttca tagaattaac taaagcaagt ttactgtttt ctaattcttc
     1561 atcagatata tcacctgctt ttacactttc tacttgtttt tttatcaatt ctacagcttt
     1621 atcataatct ttagtttcta taccagatga tataaacatg atacttttat acttctctat
     1681 tgaagagaat atatagtaac ataaactttc tttttctctt acattaacaa ataatttaga
     1741 gtgtggacca ccacctaaga cattgctccc aactactaaa gcataatatt tatctatgtc
     1801 tgcataatcc acatttgctc tataacccat aactaactta ccttgagtta tttccatttg
     1861 ttcatctata acttttacct catccacatt ttttataaaa tctgctcttg gtatatttat
     1921 aatttcttct ctttcaaatt ttagattttt acttattata tctacaactt tatcttcatc
     1981 aaaattacct tctacaacta tatctatagg tgatgtttta agtatgtttt tataatgttc
     2041 atataactct cttgaagtta tactatctat ttcatcttca taaccaaatt cacttacact
     2101 atatctctct cctttacaca tagcctcaat acatttatct tgcgcatatt cctttttatc
     2161 atttattatt gattgtatct tttctcttaa attctctttt tcaatattta aatattcttc
     2221 tttaaaccca ccatctacaa caagcgtatt atttattact tcattaaaaa attctactac
     2281 ttcttcaaat atactctcat ctaaatactt ttcacttatg ttaattactt taaaactcaa
     2341 tacttgacgt tctccacgtt ttacagcatc agctcccata gatgaaccat ataaatcatc
     2401 taatttattg gatattgctc tagcactagg atatttagcg cttccacttg ctattattga
     2461 tggtattaga gcattttttg tagtttcttt ttcatccaat agtctctgaa catataaact
     2521 tataagattc gatttaaact tttctgtttt tatcaaagta agattaatat tattacctaa
     2581 atttattatt tttttagtat taaccatatg tacctcctat ttttatttaa acatcaattt
     2641 taaaattgaa atttatttat tttttaatta tatatactca catataaaaa ccttctattt
     2701 aattatatcc tttttattat tttctaaaag caatattttt atttaaatta aatgcaaata
     2761 ttatttaaat caatatgctt taaagtctag tttacaagct tgatatatat gatgtataat
     2821 caatatgtat gtctataatt aaaacaattt tgcaatccta tagattgatt gtttaaagtt
     2881 atataaagaa tataaaaaag aataagagag aggacgatta atatgaaatt aggtatagtt
     2941 ggtttgccaa atgtaggtaa aagcacacta tttaacgcaa taactcaagc aggtgcagaa
     3001 tctgcaaatt accctttttg tacaatagac ccaaatgtgg gagttgtatc tgttccagat
     3061 gaaagattaa acaaactaca agaattatat aattctgaaa aaatagttcc tactgctata
     3121 gaattttgtg atatagcagg tttagttaga ggtgcttcta aaggagaagg tttaggaaat
     3181 aaattcttat cacatataag agaagttgat gcaatagttc atgttgttag atgttttgag
     3241 gatgaaaatg ttgtacatgt agatggctct gtagacccac ttcgcgatat agaaactata
     3301 aacttagaat taattttctc agatatagag attcttgaaa gaagaattaa taaaactcaa
     3361 aaagctgcta aggctgataa aactttaggt tctgaacttg atttattaaa atcaataatg
     3421 tctactttag aagaaagtaa gtgtgtaaga actatggaat ttacagaaga tgaacaaaca
     3481 ttcgtaaatt cattggattt acttacatct aagcctgtaa tatatgcttc aaatgtatca
     3541 gaagatgatt tagctgataa tggagaaaat aataaatatg ttcaacaagt taaagcattt
     3601 gcagaaacag aagatgccga agttgttgta gtatgtgcac aaatagaagc agaaatttct
     3661 gaacttgatt ctgctgaaga aaagaaagag tttttagaaa cattaggtct tgaacaatca
     3721 gggcttgata agcttataaa atcttcttat gctttacttg gtttaatttc attcttaact
     3781 gctggtccaa aagaagttag agcatggact ataaaagttg gaagtaaagc tcctcaggca
     3841 ggaggaaaaa tacattctga tatagaaaga ggatttatca gagcagaaac aatagccttt
     3901 aatgatttgg ttgagcatgg tactatggct gctgctaaag aaaaagggtt ggttaaatta
     3961 gaaggtaaag aatacatagt taaagatgga gatgtaatat tatttagatt taatgtttaa
     4021 aatgattaat catatacact ttgactatta tatataataa ctaaaaaact aagattataa
     4081 taatacaata ttatcttata ataattatat agaatttaaa aataaaagaa gagtatttca
     4141 aatgatttgt ataaattatt atgaaaaact ctttttttga tataataaaa ttacttacat
     4201 atataacatt taaatttata aattatcttt ttatttttta aaatatgtat aacacaccta
     4261 tagaaattac tattattact atctaaagta ttttgctata atcccatata ctcttacctc
     4321 aaaaacattc aaatggaaaa aatactacat attaataaac tttgcccaat aattcctaaa
     4381 ataatatcct aacaatgatt tatgttttta taatagtata tttttacata aaaaagtgaa
     4441 cattgattaa caaagttcac ttttttataa atttttatgt tataattcac aattttattt
     4501 aatttgtttc ttttctattt aaactatatc tatctaatat taatttttca tattcttcta
     4561 ttggcattgg tctagcaaat aaaaatcctt gggccatatc acaacctata aaccttaaaa
     4621 actctgcttg ctctacagtt tcaacacctt ctgaaacaac ttttatatca agttgctttg
     4681 ccatttgaac aatacttgaa actacaattt gactcttttt tgcatttccc actgttataa
     4741 agaattgcct atctaatttc aatacatcaa ttggcatatc tttaagcata tttaaagaag
     4801 aataaccaga accaaaatca tccatagaaa ttttaaatcc aacagatttc aaagtttgca
     4861 ttattttaaa taatattttt gtattgtcaa atattgcact ttctgtcaat tcaatctcaa
     4921 ttaaatttgg ttcaatttca tattcttttg ttattctttc aagctcagaa ataaaacctg
     4981 gattctcaag atgtactctt gataagttta ttgatatagg aaatgtgggg aaattttcat
     5041 tttcccaacg tttaaaattt ttacaaactt gggcaaatac aaacatatcc aattctgtta
     5101 taaaaccatt tttctcaaaa ataggaataa atacacctgg acttattaga ccttttttgg
     5161 gatgttgcca acgtacaagc gcttcagcac ctacaatttt agtagttgat aaatcaacct
     5221 taggctgtaa ataaacttta aattgttcat tttttaaagc atcatgcatt tcttgttcaa
     5281 tatcacgttc ttctatgaga gtttctttta aattttcagc ataaatctca taaactttta
     5341 aatattttct ttttactgta gtttttgcaa tcatagcttt gtcaagacag gtagaaatat
     5401 ttgtttctcc ctcttcaaca aaatatacac ctatggatgg aattaaattg agttctactc
     5461 ccaaacttgt ttttatcatt gacaattttt tacgtataag ttcacatatt tctataattg
     5521 actctctatt atttttcttt tcaaatatta tagcaaaatt gtcatttgaa attcttgaaa
     5581 atacagattg ctctttaaat atattcttaa ttatctctgc tatttccttt aatactctat
     5641 ctccttcttc atacccaaac ctatcattta ctagcttaaa cttatctata tcaaaataaa
     5701 ttaaaactag attctttttc tcatgagagg ataaaaattt tcctccttca agattaaatt
     5761 tatttgaatt gccaatacct gtaacgttat ctttaaaagc aaattcttcc aatttttcct
     5821 tactcttttt atgtataaat agtatataaa ttgttaatgc cgtaaagatt ataatcaaca
     5881 ctacacatgc tgataatgta agttttataa tagcgtatga tttctcagat atagcagttt
     5941 taggaacttc agaaaaaaga taccaatcat tgattcctat tggggcataa cttaaataat
     6001 atccttttcc aaacatacta taacttaatg aacctgattt aaagtttttt atatttgact
     6061 tcatattatt gatactagat tttgatattg aatcacttct gccaatgtaa gaaaataaat
     6121 tttctttagt agagttggcg tttttgctgc ttggatttaa aacaatatta ccattacttc
     6181 tagctatata agaatatcct tttccattaa atgtaggtac agaaagtatt ttttcataca
     6241 acttcgtact atgtgtggca aatataactc ctttaacctc attatcttta taaattggca
     6301 cagaatatac attaatatta ctattggtat ttttatcatc ttctattaca tcttttaatt
     6361 tttctgttat aaccacttct ccttgcattg attttaagaa ataatctctt ttagagaagt
     6421 cttctatata accatctgta gtgtatgctg taccatttgg taaaataact cccattcttt
     6481 taaatgaact attaatagct tgtcttttta gtatagaaag gatattatct acttcaaaag
     6541 actcttctcc ctcaataaat attgaaattg actctagtaa ctttatatct ccattaattt
     6601 gtttttttaa cacattgacg ctttgatttg ctatttctga aagatattga ttcgtttctt
     6661 cacctaatgt cttattcaac cttgaaccat aaatcaaaca ggaaattgta attactaaga
     6721 tacttatgac agcaattatg gacaaaccat acctattatt ttttgcatca cttaacatta
     6781 tgcttcctgc ctttctcgaa tctatgtatg tataatcatt atcttatgac aattattgta
     6841 aacattttat aataatactt atttttccat atttactgta attatacaaa attataaact
     6901 ttattacaac aataaattaa ttaatataga tattagggtt tatattattg cattatctat
     6961 tttacaatca tttacatata aaaaaatgta atatcaaagt gaaacttgat attacatttt
     7021 tgttcttgta ttttgtttaa tttttatgct acattttact taaacactca tttgcaactt
     7081 ctttatcatc aaaatgatgc ttaactttac ctataatttg ataagtttca tgtcccttac
     7141 ctgttataat tacaacatca tccttttttg ccatacttat agcttctgct atagcttgtt
     7201 ctctatcaac aacaacatgg taattttctt ttttcttgtc aattccttca agtatatctt
     7261 ttattattgc ctctggttct tcagttcttg gattatcaga agttataata cataaatcag
     7321 aatatttttg tccaattgct cccatcaaag gtctcttttc agtatctcta tctccaccac
     7381 atccaaacac agaaattatt tttccttctg caaattgttg agttgtcttt agtacatttt
     7441 caagtgcatc aggagtatga gcatagtcta caattacact tataccttta tcatttggaa
     7501 ctgtttcaaa ccttcctgat actccattag aaagtctaag accttcttta tatattggtt
     7561 taggtatgcc aagtacatag caagcagcta taactgctaa agtattatat actgtaaaca
     7621 ttcctggaac tggtataaat attacttctt catatgaagg tgttattaat ctataagata
     7681 ctccatccga atctgatttt atatctctag ccataaaatc agccttagta tcaacaccat
     7741 aagtgtaaca aggtacattt atacctttta tattttcata tatctttttt ccaccttcat
     7801 catctatgtt tattatattt gccatagtag tcttaaaaaa taatttttct tttgcttttc
     7861 tataatcctc taaatcttta tgaaaatcta aatggtctgg tgttaaattt gtaaatatgc
     7921 ctagtttaaa gtctgtttca tcaactctat ttaatgccaa tgaatgtgat gaaacttcca
     7981 tcgcacagta gtcacatcca ttatccaaca tattattaaa atgatattgt aaatctatac
     8041 tttctggagt tgttgaattg gaaacaatgt ctttttctcc gtcaaaaatt tttatagttc
     8101 ctataagtcc aactttattc ttatttaaag ttaatatctc atttagtata gtagttatac
     8161 tagtttttcc attagtacct gtaactccta taacgttaaa tttctgagat ggatgattat
     8221 aaaaattatc agctactttt gccatatctt ttcttgtatc ttttaccttt ataaatgtac
     8281 atccttttat ttctacatct tcttcaacaa gaaatgcact tgctcccttt tctatggcat
     8341 cttttatata cttatgacca tctgacacaa atccttttat acatataaat aatgtacctt
     8401 ttgtaacctt tcttgaatta tattgtacat tattaatatc tatatttaat tcacccttga
     8461 cattgattat atccaacccc tgtataatat catttaattt cataaatgct ttcctccctt
     8521 taaactcttt ctgctaaaat tacactacat ataatattat attaagaatt ttttactatg
     8581 tgaatggtta aaaatgcttg aaatattgca aaaaatgctc tataattttc tataatataa
     8641 ctgaggaaag gtcttctata aaaactatgg ataataaaat cgggtactta aacgataact
     8701 ttaaaatatt taatattaga gataaaaaag atatacaatt tgaataccat aatcatgatt
     8761 ttaataagat aattatattt ataaatggaa atgtaactta ttttgttgat ggtattccct
     8821 ataaactaaa gccttgggat atactcttta tcagcaataa tgaaatccat aaaccagtta
     8881 tagattcaaa tgtcttttat gagagaattg caatatggat tagcccagaa tttatgaaaa
     8941 aaaatagtga tgctactagt gacttagaaa aatgttttaa aatagctacg gagaataaat
     9001 gcaatagatt gcgtttaaat atacatgata ttgaatctat taagagtttt attgagcaaa
     9061 taaatatttc cgaatccaat actgaatttg gaaatgaaat attgagaaat gcattattta
     9121 tacaacttat gatattctta aacagaattt tcctaaaaaa tgaaaatatg gaatcaacag
     9181 aagacgtttt ttatgatgaa actattaaga aagtattaaa ctatataaat tcaaatatag
     9241 atgataagct ttcaatagat agcatatcgt ctgaattttt tataagtaag tattatctta
     9301 tgagaaaatt taaatctcat actggaacgt ctatacacaa ttatatagtt caaaaaagat
     9361 taattttagc aaagtcacta ataaccaatg gttaccttat gagtgaagtt tgtaataaat
     9421 gtggattcaa cgattattct agttttgtaa gggcatttaa aaaaaactac ggtgtatctc
     9481 ctaaaaatta tataaacaat aaaaatcctt tagatattac tcttttagat gaatagataa
     9541 tttatgtaaa ttttatttta ataaaaactt gaagtattag tgtatttatc ttactaatac
     9601 ctcaagtttt ttaatgtttt aaaatatatc aaaatataat gatatatatt tctattactt
     9661 aaaatatata ttaaataaat tgcatgttaa ctgaacctat ttatatattt attaaatctc
     9721 acataacttt tagttatact atcatatagc tatcctattt tttcaatatc atatcttaat
     9781 atacttactc tgttctcctc ttttgctgta gttattcctt ttttaatgct taatttctga
     9841 ttttgttcta aattcgtaaa tactacagga cttattgttg atagacaatt tttttcaata
     9901 tattctaagt ccattttaac taatttttct cctgctttta cttcatctcc tacattcaca
     9961 aatacttcaa atccatttcc ttctaaattt acagtttcta tccctaaatg tataagtatt
    10021 tctactccac tttttttgga ttttattatt attgcatgct tagttggaaa tataactcca
    10081 atagttccat caactggtga ataaatcaat ccatcactag atttaattgc aaatccatcc
    10141 cccatcagtt tacttgaaaa tacagaatct ggcacttctt ctatatctaa tatctctcca
    10201 tttgttaatg aatcaatctt ttctactaaa gcattttcca tattggcaac ttccatattt
    10261 tttttcactt tatttttatc aacattttta gtaacagtac tttcaacctt tgtttttccc
    10321 tctattatat cttttatatc agactttatt gaatccgatt ttggtccaaa tatagcttga
    10381 acattgttgc caataaccat aacacctgct gctccaagta atttcaattt ttctttatct
    10441 actttattta tatcatttac aaccaatcta agtcttgtta tacacgcatc taggctcttt
    10501 aagttgtctt tatttcctaa agcaattaaa atctctgatg ctaattcatt cttagacatt
    10561 tcaatgactt cagcgttagt ttctgactca tcttctcttc ctggtgtctt taaattaaat
    10621 tttcttatca taaatctaaa tacactatag tataatactg caaaaccaat acctaccaag
    10681 atagccaagt accatggagt tctattagga agaacaccaa ataataagaa atctattaat
    10741 cctcctgaaa atgtgaatcc aattttaact cctaataatt gcataattgc gtatgataaa
    10801 cctgaaaata aacaatgtat tgcaaataat actggtgcaa caaataaaaa cgcaaactct
    10861 attggctcgg ttattcctgt taaaaaagag gttaatgctg ctgatgccat tatacctgct
    10921 acaaattttt tcttttctgg ctttgcttca tgcaccatag caagagctgc tgctggaaga
    10981 ccaaacatca taaatggaaa tgcacctgtc ataaaagtac ctgctgtgaa atttactcca
    11041 tctttaagtt gagcaaagaa tatcttttgg tcacccatta caagctcacc agctttattt
    11101 acatattctc caaattgata ccaaaatgga ttatggaaaa catgatgcaa accaaatggt
    11161 attaatagtc tttccaaaga accttccata aatgctgcta gtgtttgatt tgcttctata
    11221 acattttttg agaaactaag taatccctct tgtattggag gccaaataaa agacaatata
    11281 attcctattg ctatagcaac taatgaagta acgattggta caaatctttt acctgcaaaa
    11341 aaacctagat actgaggtaa ttcaatttta taaaactttt tataaagata agcagccact
    11401 atccctataa taataccacc aaatacccct gtttgaattg ttggtattcc aacaatactt
    11461 gcatacatag aattgtctac catatcttct gtaacacccg ccataacacc catagatgta
    11521 ttgagaatta aaaaacctac tatggctgcc aatgctgcaa caccttctcc ttcagttaaa
    11581 ccaacagcta ccccaactgc aaatataagt ggtaagttat taaatataat ttgacctgaa
    11641 ctttccatta tacttattat aagttgaaat gtaggattcg ccaaagctgg aaaatgagac
    11701 aacaatgcgt ctcctgctaa agcattactc acgccaagta atatacctgc tgcaggaagt
    11761 aatgcaactg gtagcattag agattttcct actttttgta aaacaccaaa tacttttttc
    11821 atttactttt ctctccttat tattttatat ttttttaaat aaaaaagact taagtttata
    11881 aaaagttaca ttagtatact taattctaaa attaaactta t
//
LOCUS       DAGQWR010000127        11817 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.127, whole genome shotgun sequence.
ACCESSION   DAGQWR010000127 DAGQWR010000000
VERSION     DAGQWR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11817
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.127"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            82..1056
                     /locus_tag="MIW95_003029"
     CDS             82..1056
                     /locus_tag="MIW95_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152337.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYKLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNHPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(1172..2203)
                     /locus_tag="MIW95_003030"
     CDS             complement(1172..2203)
                     /locus_tag="MIW95_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBY3152338.1"
                     /translation="MKKIRKLATKLLITTIVLVSGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNNKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPTVGAVAQTQKPALPGKHTMLPHQDGAK
                     VVGKFGDIKDKHVMYEGVRCVVINSSVNTVTFLGSKSNLDPSNLTIAVIGFMNTPYGM
                     FALAPEGEPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIEITDEKANIGL
                     GQIMYIRYE"
     gene            complement(2815..3666)
                     /locus_tag="MIW95_003031"
     CDS             complement(2815..3666)
                     /locus_tag="MIW95_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY3152339.1"
                     /translation="MSCILKLKQIFEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(3683..4603)
                     /locus_tag="MIW95_003032"
     CDS             complement(3683..4603)
                     /locus_tag="MIW95_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBY3152340.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDGIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(4713..5627)
                     /gene="murQ"
                     /locus_tag="MIW95_003033"
     CDS             complement(4713..5627)
                     /gene="murQ"
                     /locus_tag="MIW95_003033"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBY3152341.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNRVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(5726..6823)
                     /locus_tag="MIW95_003034"
     CDS             complement(5726..6823)
                     /locus_tag="MIW95_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBY3152342.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEKALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFDFKPSTK"
     gene            complement(7056..8402)
                     /locus_tag="MIW95_003035"
     CDS             complement(7056..8402)
                     /locus_tag="MIW95_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY3152343.1"
                     /translation="MLDKFTEFLDSKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(8491..8799)
                     /locus_tag="MIW95_003036"
     CDS             complement(8491..8799)
                     /locus_tag="MIW95_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3152344.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEASVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(9060..9449)
                     /locus_tag="MIW95_003037"
     CDS             complement(9060..9449)
                     /locus_tag="MIW95_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152345.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSNKNTKKEMEKNQRTDYNIPTISFK
                     SGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRGFSL
                     GFFRLYILFVLSFAIIGYFWIYKGIHI"
     gene            complement(9451..10611)
                     /locus_tag="MIW95_003038"
     CDS             complement(9451..10611)
                     /locus_tag="MIW95_003038"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBY3152346.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAIIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDKNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGVHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(11041..>11817)
                     /locus_tag="MIW95_003039"
     CDS             complement(11041..>11817)
                     /locus_tag="MIW95_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY3152347.1"
                     /translation="YLIDGQSFEENLIKDIIENLKIKNNSDIETYKQSLKELDDYIAK
                     WVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKINFLVNSILYSSRSNTMFEDVFIN
                     KFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDVYTDSKCMSYVLDQIINNAIKYSK
                     EVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRVFDKGFTGKNGRNQLYKSTGMGMY
                     FVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDYLSLDS"
BASE COUNT         3768 a         2080 c         1313 g         4656 t
ORIGIN      
        1 attaagtata gttacttacc tttcaagcaa tattagtttt atgttagaat tttattatat
       61 tgatttacag gaggtttgca tttgaataat attaattcac ttaaagtaat aaaattatct
      121 cttggatgtt ctcttgctat attcattgct tggctattaa aattagaata ttcaatggtt
      181 gctggagtta tagtactact tacagtaaag gacactaaaa gagaaactct taaaggctcc
      241 attggaaaga tttatggttt tcttttatgt actatttttt catatttatg ttttaatatt
      301 ttaggatata atttgacttc attttcaata tttatattta taattatacc actttgtttt
      361 ttgttaaata ttcaagatgt tattgctatg tgtgtagtca tagcatccca ttactacctt
      421 caaggagaaa cttctatcaa atggatttta aatgaagctg gaatatttgc agtaggaact
      481 ggaataggtg tcttaataaa tatgtatatt ccaacaaata tacataaaat ccatgaagtt
      541 caaaaaaagt tacaggaaga agtgagtatt gtacttatag acattgcaga tattattgta
      601 aatcctaaaa aagaaaatgg atatagtaga gatttatata aattaaattc acttattgac
      661 agcagtattt ctgaaactta cgataatatt aataatactt tattaagtga tactagattc
      721 ttcttagaac atatggatat tataaaaagc caacgggata tcttagaaaa tttgtatagt
      781 tatgtatctc aactaaattc tacaccacca caagctcaca ttctatctgc tttcatacat
      841 aaaataggtt atactgaatt tgaagctgaa acaggcaatc ttcttcttga agaattgaag
      901 agattaatgc ttagtatgaa aaaccatcct ctacctgttg acagaacaga gtttgaaaac
      961 agagctattt tattcctatg cttgactgag ttaaaacaat ttttagttaa tcgtaaacat
     1021 gctcagatgc ttagagataa taatttttat aaataaaaag gtgagaaatc cagcagttga
     1081 cctgactaca aaatatcaga tataatatta aatgcgcccc tagtaagagg gcgcgatgtt
     1141 tataacttat tctactgtca tgattaaatc attattcata tcgaatatac attatttgac
     1201 caagtccaat attagctttt tcatcagtga tttctatttt aatagtcaca tcattttttt
     1261 tacagtactt tgctaattct gcaccagaac gatttttacc actaaaatct aaagaaaatg
     1321 gcttatcata tttaaaagga tattcaacag gttcaccttc tggtgctaat gcaaacatcc
     1381 catatggtgt gttcataaat cctattacag caattgtaag atttgatggg tctaaatttg
     1441 atttactacc tagaaatgta actgtattca ctgatgaatt aattacaaca catctaacac
     1501 cttcatacat tacatgttta tcctttatat ctccaaattt tccaacaacc tttgccccgt
     1561 cttgatgtgg taacatagtg tgcttcccag gtagtgctgg tttttgcgtt tgtgctactg
     1621 ctccaactgt tggatatgct gtcttatcaa atggtaatgg gtggctaaga ctcttgtata
     1681 tgatatcctt tcctgctgat ttattagcat caatatctac atgatacaaa cgcccatcac
     1741 caaaacgtat aagattccac tggtggtcat ttgatgtgct gtgtacataa taagattcaa
     1801 ttcccatagc gtcacacaaa gctttcatgc tcctagccat tcctgaacat gcaccttttt
     1861 tagtaaccaa tgtataggca gtattcgctc cttctactgc attccagtca acatatgttg
     1921 tgttattttt aatgtgatta taggcaacta aaatcttagc gtctgtagtc atgttctttg
     1981 taattttatt gttcataaaa tctgctgttg cttgtgctac tgagtttagt tgggcttgtg
     2041 acagctttgg accatatacg ctattggttc cagtgacata gctttttggt gtttttgctg
     2101 ttacctcttt ggctgtcata ccataaactg tcatactcat acctgagact aaaacaattg
     2161 ttgtaatcaa taacttggta gctaacttcc taattttctt catacttagt attctccttt
     2221 ataaaatatt gttacttcct attctctacc attaataaag ttcatttggg atttaaaaag
     2281 gtggattgtc aatatgtatg tttcttcatt tatacgttcg tgaaatattt aaatttcatg
     2341 gaaaatctaa tagatatttt ctagataatt tgtatacaat acaactcgca aatcagggtg
     2401 tttgctgtta gtttgagaaa atgatgatga ttaatatatt cggaatcttt gacctataat
     2461 aaataccatt gtcttcaatc attaataatg gtaagagtaa agagaattat caataataca
     2521 gttaaaatca actttgtata catttcatac attctctccc aacaacatta tacttcttat
     2581 aatttgtata ttcaatacgt tttctgggaa aatataggat atatctataa aaatataaaa
     2641 aaggagttga acttaaaatt tgattaaaaa aaatcaattt taagacaact catttgtatt
     2701 ataaaaatgc tatatattat tattaagtaa gtcaatattt tagatattta tctatttctt
     2761 attgacttac gcttatagtc taacttattt gtttagttga tatataacct agttttaagc
     2821 attattaata tttaaataat caagtatcct ttttgtttct tgtagtttct gttctgcctc
     2881 atttaagttt tcttgaatta tagaaatata tattatatct atcaacgtaa gcattgctat
     2941 tcttgaagaa atagctcctt ctctaagcct tttttctata tttggtacta ctagctttaa
     3001 atcactaatc ttactaagat gattgtcact atatttagtt atacaaatga ccttacatcc
     3061 ttttaatttt gcattttcta tagactttat tacttctttt gattctccag agtaagatat
     3121 tgctatagct atatcttctt tatctacatt tgaagatacc ataagttgtg tatgactatc
     3181 taaagaggta aatgcctgtt tatttatcct taaaagtttc atctgtaaat ctagtgcaac
     3241 taaagccgaa ccacctactc caaatatata tacattttta gcctttatta tctgttcaac
     3301 tgcttctttt attatatctt tttcattcaa caaccttgtt tggttgatag tctctatgtt
     3361 tttgtttgta attttgttta taacactatc tatattacaa tcatagtttt catctacata
     3421 tgtgtattca ttttctttgc ccatcaaatc tttagctatc tcaattttta aatccccaaa
     3481 acccgaatat cccatttttc tagaaaacct aactatactt gctgtacttg tttttgagtt
     3541 tgacgccaat tctgatacag aaagcttact tatagcctct gtattattaa gtatgtaatc
     3601 agctattttt ttatctactt ccgttaaatc ttcaaatatc tgttttaatt ttaaaatgca
     3661 actcatactc taccacctct acctatttac tataatattt cttcttagca agtaaacatg
     3721 ctcctaaatc tggagtcaac actggtctca ctataccaaa tctcgtttta ttcaaatatt
     3781 ttttaagtgg ctctaatatt aaatctcctg acttaaacac tccacctgaa taagatacct
     3841 ttatctttgt accttgttca aaatgatgct ctaatcctat tataagccta cttaattcat
     3901 aagctgcatc atcaaatatt ttactagcag tattatcacc ttcaataact gcttttaaac
     3961 atatcgtagc aaactttgct atttctattc tatctccttg tattccatca tttacatatt
     4021 ttattatgtc ataatcattc tctatattaa gatgtctttt tattaaatca tataattttg
     4081 tctttgaaca ccttccatca gactgcattg tgaacatttt taaggtagcc attcctatat
     4141 aatacgctga accttcatct cctaatacat agccccatcc tccacatctg tgaagattcc
     4201 catctttatc taaagcctgt gctattgaac cagtacctgc aattatattt ataccttttt
     4261 ccccatttaa agaacctgct aatgctattt ctacatcatt acctagagta taatttatat
     4321 gactatatgc attttttgtt gctacttcta atgcatacaa tacttctttt atttttccat
     4381 atccagcaag tccaacaaat gcatacgtta tttcttctac atttatctta gcatctttac
     4441 aaacttcttc caacccagtt attaataatt tcgttagatt atcaaatcct atttgattat
     4501 aatgacaagt tcctttggtt atggttccta ctatatttaa ttcttcatct actaatgtaa
     4561 atgttgtttt cgttcctcca ccatctacac ctaaaaaata catactatcc tcctacaata
     4621 tctttaataa ttctttcaat acttaatatc taaaaataac cttccaatgc tgttttttta
     4681 atattgaaag gttatcagta ctattattta ttttacaatt tttctattga ttttaaagct
     4741 tcacttatat aaccttcttg tctatctagg atactctttg cactatcctt atctaaacca
     4801 ctttttatca taaatatagc caatttaact ctattatctg cttcttctaa gaatttttct
     4861 gcaacgtcat attttacatt tgtagctatc attattattc tttttgctct ttcaaccaac
     4921 ttttcatttg atgtcttaac atctaccatc aagtttgagt atacttttcc catcttaacc
     4981 atagaagctg ttgttaacat attacatacc attttttgag ctgtaccact tttcattcta
     5041 gttgaaccca ttattacttc tgcccctact ataatgttta ttggtaaatc cgctttttta
     5101 ctcatttcac tattcggatt catagttatt gatagtactg cacaccccaa ttccttagca
     5161 tactccattg cacctataac ataaggagtc cttcctgatg cagctatacc acatataaca
     5221 tccttagatg taactcctct atctattata tcttgctttc caagttcttt atcatcttca
     5281 gcaccttcta ttgctttaaa tattgcttga tttccaccag ctattatagc ttgaacaagc
     5341 tcaaaatcca ctccgtaagt tggaggacat tctgtggcat ctagtattcc tatcctccca
     5401 ctggtacctg ctcctatata gaataatcta cctccctgct tcattctatc tactattata
     5461 ttaacagctt ttgctatact ctccttttgt tcttcaacag cataggctac agttttatct
     5521 tcgttattta ttaattctat tatgcctaat gtatcttctt tatctatttg caatgtattc
     5581 ttatttctac cttctgtaac taaattttct aaagttttta ttaacatatc atttcctcat
     5641 tttcttaata ttaaattttt aaacacattt taactccaac tttcataatt atatttttat
     5701 aataaattaa tattttcata ataaattatt ttgttgaggg tttaaaatca aacttttgcc
     5761 atggtttaat ataatctaat ataaataatt cttcttttct tatatatcct actacattcg
     5821 atttaccact gtttttcata tcagtcaagg ctatttgtaa ctcccctgca taatgaccat
     5881 attcactact ttcaattact atatctcctc tttttattat tttaggagta ttaaatagtt
     5941 caaatttgta tcctttatat ttaacacgag attgagtaga ccttatcaga ctgtcagata
     6001 tatcccctct attaaaatgt atttcttcaa agagtatctt cttctcaact tctcgtatat
     6061 tttcctctat tgctacatca aatgtaacca tatcttttct catagaacct aataccttta
     6121 gctcctcata tgttggataa caatttgata ttattatatc atttatatta ttcatagcta
     6181 ttatatgctt tacttgaata tctataggta gatttctgtg catctcaatt gttggaagcc
     6241 catcagttac aggccatggt ccaaatgtgg tttcattttg acttgttaca aaaactgctg
     6301 ttcttaatcc atacttatta aatctttctg tacattttat aaagtgttct aaatttaatc
     6361 cactatatct atgtggataa aagttatgac atcctgtcaa attttcttta tttggttggt
     6421 agtccattat tgtatctata taattagtat tattactcat attgatttct attttaagat
     6481 tttgaggatt aaaagtcatt aaggcctttt cagaacctgt aaatcccata tcaagcctta
     6541 ttccgtctgc cttcgtcttt ttaaaaaaag tcaaatcttt gtagcttatt tctaattcac
     6601 caaatactct gggatttaca tcaagtatta cctcaaaacc tttaaactta gcgtattcat
     6661 ttatactttt aaatttttta attatatttt ctttagtatc tttaactgat aataaacatg
     6721 aaaatatacg tgtaaatcca ttttcagcag ctttatctat ataatcatat atttccttag
     6781 atgttgtttt ttctggataa attgatattc ctaatcttcc catctgtgtc atctccttat
     6841 atcctatatg tgtttaaatt tatttcgaat tacacgaaac ttttttaaaa atttggtgaa
     6901 aaaaagtttc atatatattt ataaaaaaag aaaagtttac cttttctttt tttataaaca
     6961 atcaacaaaa tatcttcgtt acttatttca taactgttat actttgaaat ttatactaat
     7021 ctaatatagt ttttaatttc caatatagtt tctaattaat aagcttcttg ctcttcaact
     7081 ttttcttcat gaagcaatct cttatcgtac atcttgacaa atggatagta aattattatt
     7141 gatatagcta tacataataa gttaagaact gaagctctcc aatctccacc agtggctaaa
     7201 tatgccccta ctggtcctgg taatgtccaa ggagcagtaa ctactactct ggatactaaa
     7261 ttagcactca tagcaaacca tgaaactgtt gccattacta ttggtgccac aacaaatggt
     7321 attattaata ttgggtttaa tactattggt gctccaaata ttattggttc atttatattg
     7381 aatattgctg gtattacagt tgcttttcct aaagactttc cataagctga ttttgctcta
     7441 aatgcaagaa gtaaagctaa gcctattgta gctccagaac ctccaatcca tataaaccat
     7501 tgaaaaaagg gttcagcagc aatacctgga actgcttgac ccgcagcata tgaagctgta
     7561 ttagcatcta ataattgtat ccataaaggt cttgccaatg aacctattat tgatacacca
     7621 tgtattccaa atgaccaaaa aaacgttatt aaaaatatta gtataattac tgatggtaat
     7681 gtgtcagtgg ctgaaactaa tggagttact aatttagcta tgaagccatg ccaatcaaac
     7741 cctaggaaat aagtaacaat agccattaaa attattatta atgcggctgg tgtcagagct
     7801 tcaaaagacc ttgtgactga tgctggaact gcttctggca tggttatctt gaatttagat
     7861 ttgtcagtaa atctataaat ctcaacacct attatagatg ttacaatagc aacaaacatt
     7921 gaacctccac caagattagc cattggcatt tgccatcctt gaggaattga attataccaa
     7981 gctaaaagtt ctggactctt ttcaactatc tttaataaat caggggtaag accctctatt
     8041 gcagtaggaa ctattgtcaa aaggaatgta gctgtagcta ataaacctcc tgtcaatcca
     8101 tctaacttat atgactttgc caaactataa cctatgccaa atgtagaata tactgatata
     8161 ataaaaaatg tcattctata tggtagtaat attgtaactg cattttcctt tagatactga
     8221 gttacccccc atgaatctgg taatggagga aatgctaata tcaagaaaaa tgacccaact
     8281 attataagtg gtaatgaagc tacgattcca tctcttacag ctcttaagtg acgttgttct
     8341 gccaatctgg ccatagaagt tgataattta ctgtctaaaa attctgtaaa tttatctagc
     8401 atcttttctc tccttaatta atatttattt ttattatcat taaaattttt ataaaattaa
     8461 ctttcttcta acctaacaac actgcaacta ctatttactc aacaactctt gaacttgttt
     8521 taataacttt ggtcctccta atggactata agcttgtgct tgtattaatg cacatggtac
     8581 actagcctca tcagcaaaag cttttaaata gtcaaatcta tgtctgactt gtggagctac
     8641 catagcaata tcccatccat ttttaacttc attctcaaac tcttgagtac caacagccaa
     8701 aacatctata tctattccat tttttgctcc ttctttttgt aatgcattaa ctgctatagc
     8761 acttgacatc cctgatgaac aaacaaataa tactctcata ttgattcccc ctgttttttt
     8821 atttcttttc aatcattata gcatgtattt ttgattacag gaatacgttt gtatatattt
     8881 tttcatattt tacattttta attgtaattt tatttcatgc atttatatct tgctttaatt
     8941 gctttattta tctaatcatt cttttgtcta aaattacaca ctacttaaaa tactcatatc
     9001 ttttcttaaa aaagaaaaaa gactatcttt tattaacata aaatagtctc aatacttttt
     9061 taaatatgta ttcctttata tatccagaag taacctatta ttgcaaatga taaaacaaat
     9121 aaaatataca gtctaaaaaa tcccaaagaa aaacctctct tagtttcaac aaagtatctt
     9181 gtatatgcaa ctgcaaaaga tgtaattaat gaatttccta taacaactcc aaatctatta
     9241 tctattccaa atatagataa taacaaagga aaaactacag tacttactag agatgaaact
     9301 attagtatta atccactttt aaaacttata gttggaatat tataatctgt tctttgattt
     9361 ttctccattt ctttcttagt atttttatta ctttttgcat ttttataatt attatttttc
     9421 tttcttttct tttttttatt attagacatg tcatttacct ccaaaaggtt taggtgttat
     9481 attccctaaa ataactttat ttttagcccc tgttgcagat attacattag aaaaagaatt
     9541 atttaatgtt tcattgccca agattacaaa tgccaaagcc tcctttgcat cagaagaata
     9601 cccatattca tcttgagtta atacttcaac ctctaataat aactctttta tatatcctat
     9661 taaagttaga ttatgaactc ctccacctcc aattaataaa gtatctacat taacctttgg
     9721 aagtatgaag tttttataat tataatatat agcttttgcc gtaaacatag ttactgttgc
     9781 tatcaaatca tatttatcta catgtttata tttgttaagc atgttatcca aatagacttg
     9841 tccaaatacc tctcttccag tgcttttagg tggcgcttga ctaagatact tatgactcat
     9901 caaatctttt aacatttctt cattaatttt accttttgat gcaaagtatc catttttatc
     9961 atactttcta ttaaataatc tttgacatac tccatctatt atcatatttc ctggtcctgt
    10021 atcaaatgca aatacatcat ctatgttaca tgatttaggt attatagtaa tatttcctat
    10081 tccaccaata ttttgaagtg ctaaattttt atttttatca ctatataata aaaattcact
    10141 atatggtact aatggagcac cttctccacc tgctgctata tccattactc taaaattaga
    10201 tattaccttt gtatttgttt catatgctat aattgctggt tcacctattt gaagcgttga
    10261 agatacaaaa ttattataac ttcttggtat atgaaagatt gtttgaccat gagatgcgat
    10321 aaaatccaaa tttgctatat gaaagtttgc tttatgacac actgatttta cagctttact
    10381 aaataaatat ccaagcttaa aatttaagct acagattaaa tcaacactcg attcctcttc
    10441 tatacagcac tttcttattt cgtcttttat atctttagga atttctagag ttataaattc
    10501 tatttgctta actttagtat ttctaccatt cccactaatt tcagctagta ctgcatcaac
    10561 tccatctaag gaagttcctg acattaaacc tacactatac atagttatca tccttttaag
    10621 tttatagtta ttcctcccca aatcaaggta ttaagtttaa taattttaga attaatctta
    10681 tttcatcttt aattgcgata atttactctt acgaaattaa tcgagttaat cttatacacg
    10741 ttttttacct tagctaaatc ttacattaat ttattttcag tgtcaatgta atgcatgtaa
    10801 ttttttttca caaacaaaac atattctctc tccaaaaacc atattaaaat agttactaca
    10861 aataaaaaac ataccaggta cctatctgat atgtttttca tatataataa ttattatttt
    10921 ataatataga tagaacatca tttaaggcac taattaaatc ttcactattt tcaagaacta
    10981 aatttattct tattgtatat gtcgatttgt atatgatgat agattttata gtctaataac
    11041 ctaactatct aggcttaaat aatccgatat atcataaaag tagatattaa aaattgtata
    11101 tgaaccaatt ttcgaacata cctcaatctc atgccctaaa ctatctatca ttttctttac
    11161 aaaatacatc cccattccag tagacttata caattgattt cttccatttt tccccgtaaa
    11221 tcccttgtca aaaaccctag atatatcttc ctcatttata cctataccaa aatcttttat
    11281 agataatacc actccatttt ctaacttttt tgaattaaat tcaatcttac caacttcctt
    11341 tgaatacttt atagcattat taataatttg gtctaaaaca tatgacatgc atttagaatc
    11401 tgtgtaaaca tcattttcta attcattcaa actaacttct acattatttt ttataagcaa
    11461 aaatgaatta ttttttatag acatttttac caatttttct aaattaaatt tatttataaa
    11521 tacatcttca aacatagtgt tgcttctact actatacaaa atagagttta ctaaaaaatt
    11581 tatctttgca acctgattct taatatccaa actatcttct atgctactta acctatctgt
    11641 aattatactt aaagaagata ttggaatttt tatttcatga acccatttag ctatgtaatc
    11701 atctaattct tttaatgatt gtttataagt ctctatgtca ctattatttt ttatttttaa
    11761 gttttctata atatctttta ttagattttc ttcaaaagat tgtccatcaa ttaaata
//
LOCUS       DAGQWR010000128        11792 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.128, whole genome shotgun sequence.
ACCESSION   DAGQWR010000128 DAGQWR010000000
VERSION     DAGQWR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11792
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.128"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(200..853)
                     /locus_tag="MIW95_003040"
     CDS             complement(200..853)
                     /locus_tag="MIW95_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY3152348.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKFMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(1098..2963)
                     /locus_tag="MIW95_003041"
     CDS             complement(1098..2963)
                     /locus_tag="MIW95_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HBY3152349.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDIPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVDENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2968..5388)
                     /locus_tag="MIW95_003042"
     CDS             complement(2968..5388)
                     /locus_tag="MIW95_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBY3152350.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNIDNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYEQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5402..6844)
                     /gene="glgA"
                     /locus_tag="MIW95_003043"
     CDS             complement(5402..6844)
                     /gene="glgA"
                     /locus_tag="MIW95_003043"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBY3152351.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNRDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6873..8003)
                     /gene="glgD"
                     /locus_tag="MIW95_003044"
     CDS             complement(6873..8003)
                     /gene="glgD"
                     /locus_tag="MIW95_003044"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBY3152352.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGS
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(8005..9165)
                     /locus_tag="MIW95_003045"
     CDS             complement(8005..9165)
                     /locus_tag="MIW95_003045"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBY3152353.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKRSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            complement(9936..10979)
                     /locus_tag="MIW95_003046"
     CDS             complement(9936..10979)
                     /locus_tag="MIW95_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBY3152354.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            complement(11000..11446)
                     /locus_tag="MIW95_003047"
     CDS             complement(11000..11446)
                     /locus_tag="MIW95_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBY3152355.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
BASE COUNT         3718 a         2134 c         1152 g         4788 t
ORIGIN      
        1 ttttaattga taaaataaat tctatattta tgatattaaa attctataag ttcattgatt
       61 tatatatgtt aatttaaata tattattcta tcgtagtgcc ttagagtttt tatttgtatt
      121 taaagttaaa tttattcttg tttttccttt aatatttaat agtaaaataa caaaattata
      181 aaaaaaataa agagaaactt tataaaagct tctctttttt catctctgct ataggtatcg
      241 ttttccaatg ttctctcaaa actctttcaa cattttctaa atcattctcc ctcataaatt
      301 ttactacttc aatatgttga ttattgctgt atgtcaacat tttatataac tcatcttcat
      361 tatcactagt atatgacttt gacataaaat tatatcttaa agagttcaat agatttacta
      421 acacctgatt atctgattta gttatgtata tatcgtgaaa ttcattactt aattttaaat
      481 agctattgta atttttatac tttatagaga ccgccatcat ttctgttaat tcttccattt
      541 ttactaggtc tgattcagtt aaattttgta atgctgaaga accggctaac gcatccaata
      601 ctccaattac ggaataggtg tctaatttct ccttttcctg aaattctttt actacaaatc
      661 ccttattagg tattttttct agtaaatttt cacctgctaa cctagttaat gcttctctag
      721 ctggtgttct actaactccg atagatttac aaatctcaga ttcagtaatt ctctgattag
      781 cttcatactc tccattttgg attttctttg atatatagtc atatacttga gttgtaagtg
      841 gatctacctt cataagacgt tcctccatat taagattata aaagttaaat ctccctacaa
      901 ctttggtaga gagttcagtt ttatcttact aatttatata atatcacaat tatgatattt
      961 tttatacttc tttgtaatat tttgaaacac taaaattctt atgataattt atacaaaaaa
     1021 agtagaaaaa gttttgataa aacaaaactc tttctacatt caccttattt tctatttatt
     1081 tttctaaaaa aagatattta atttactcta aaaattctaa atgatttaga gtctaaatct
     1141 actttcaact cattattttc aatttgtata gtactatttg catttttaag actatacttt
     1201 ataggtattt cctcaataaa actactctct atacataatg atatactttc cacattttca
     1261 cttctattta ctaaaattaa cattctatca ttattttcat caactctaat aaaagaaaat
     1321 acagaatcat tattagtata tataaattct gtatttccag aacttaaaag tttattctta
     1381 tttctaattt ttataacatc tctataaaaa ttaagcatat cttcgtcttc atttttccat
     1441 ggataagttc ttctattatc tgggtcaaca cctccacaaa gacctgcttc atctccataa
     1501 tatatatatg gtactccttc aaatgtcatc tgtatagcta cagcaagctt cactaaatct
     1561 acatcatcat ttaattccgt tttagctctt gtaacatcat gtgttccaat taaatttaaa
     1621 ttagacctaa atgattcttt tggataattt tctttaatct gcatatatct attacaaagt
     1681 tcatatgaat taatttctcc ttttaagaaa gaaaacatgt tatttctaaa aggataaccc
     1741 ataacagaat ctaattcttc acctaataaa taacttcttc tctgaccata gctaatctta
     1801 tttgatgcat cttcccatac ttcacctata agaattgagt catcatctgc cttttttaat
     1861 tcctttttta actctcttat aaacttagtt ggtaattcgt ctgcaacatc tagtctccag
     1921 cccttaatac ccatatttac ccatttattt ataacgctat cattgtcata tattatataa
     1981 tccatatagc tatgttctaa ttcatttata tttggcaatg tcttaacatc ccaccaactt
     2041 ttatactttt gaggaaattc ttcaaacata taccatgaat aatatggaga ttctttagat
     2101 tgatatgccc ctaaactatt atagtttcca tacatattaa aatatttact atctgctcca
     2161 gtatgactaa aaactccatc taatactatt gatatacctt tttcttttgc tttatcaata
     2221 agttctttaa atgtatcttc atccccaaac attgggtcaa tttttttata gtcacctgtg
     2281 tcatatttat gattactaga agcttcaaat atagggctta gatatagtat acttacacct
     2341 aattttttta aatatccaag tttattgatt atccctctta aatttccacc atggaaatcc
     2401 catcttataa catccccttg actatccttt atgtacatag gtatatcttc ccaatttcca
     2461 tatatgaaac tatttttttt aggattatca accttaccac tacgatttcc attattgaat
     2521 ctatctacaa atatatgata aagtattcct tctttgtacc atgttggtac tttaaaatct
     2581 ttgtaaacag tgatttgata tttattaata tcactataat tatattcaca agaatatcca
     2641 ttatctctgt tttttccata aaataaagtc tttatctgac catccatttc aacatcaact
     2701 ttaaagtaat aaaaataaac acctgtacca tcaaactttt caatcttacc acaaaagcac
     2761 tttccaagtt tatcattatc taattcttca tttaaagtta ttgttctcat ttcttcttct
     2821 ctatttattt ctagggaaat agacttgata ttgtatccct catttacttt aacacaaatg
     2881 gtcaattcct catcaaattt taaagctcca aacggagcct taaaattttt atcccaagag
     2941 ttatattcta ttacactttg catttaatca caccctttta aaccaaatgt cttttgcata
     3001 ctcagaaatt gttctatcag aagaaaaaaa tccagagtta gctatattaa ctaaagacat
     3061 tttattccat ttttctttat ctctatataa cctgtttata tctgcttgag cttgaccata
     3121 attttcaaaa tctctcaata caaaatactc atcattgtaa gtagtgagtg aattataaat
     3181 acttcttcct tcttcgccaa gattaggaat aaatccattt attaaatcat ctaccaccct
     3241 ttttatatcc atattagaat gatataagtc aagtgaagaa tatccaccat atttattata
     3301 atttagtact tgttctgcac taagtccaaa taaaaacata ttttctttac caacctgttc
     3361 acatatctct acatttgcgc cgtcaagtgt agccaaagtt atagcaccat tcatcataaa
     3421 cttcatatta cttgttccag atgcttcctt agttgtagtt gaaatttgct cactaacatt
     3481 tgctgctggt attattattt ctgctaatga tactccataa ttctctaaaa atactacttt
     3541 taacttgtcc tttactctaa catcattatt tatagtactt gcaacagaat ttataaattt
     3601 aattatacat ttagccaaat aatatcctgg tgctgctttt gcaccaaata taaaagttct
     3661 tgggtcaaga ttcaaattag gattatcaag taattcatga tacatatgaa gtacattaaa
     3721 tatattcagt aactgtcttt tatacgcatg caatctctta acttgaacat caaatatcga
     3781 acttgggtct acatttaaat cgtatttatc ttttataaac tttgctagat ttgctttatt
     3841 attgtatttt atatcatcaa gtctttgtaa aacagaggaa tcatttttaa atttttcaat
     3901 attttttaat tgtaaggtat ccttcttcca tgaatcacca attaaatctg taattaaatt
     3961 gctaagttga ggatttgaac ttataagcca tcgcctatgt gcaattccat tagtcttatt
     4021 attaaattta tttggttcat cttgatagaa atcttttaat acctctgttt caagaatatg
     4081 agtatgaagc ttagcaactc catttacact atgacttgtg acaatacata agtttgccat
     4141 attgatattg tcacagtcta ttatactcat tctttttatt ctatcctgct catatccttt
     4201 attatttaat tcctctacat atcttctatt tatttcttct attatcatat aaattcttgg
     4261 taatagttct ttcatcatac ttacaggcca tttttccatt gcttctgaca ttatagtatg
     4321 attagtatag gacactgttt ttttagttat ttcccatgct tcatcccaag ataaattttc
     4381 ttcatcaagc aaaattctca taagttctgg tatgcataag gtaggatgtg tatcatttat
     4441 atgaattgct accttatcga ataaattatc aatatttaat ttgttctttt tatattttct
     4501 tattatatct tgtatccctg cacttacaaa aaagtattct tgtttcaacc tcaacaactt
     4561 acctgcatag tttgaatcat caggatataa tacctgtgat atttcctcag tataatactt
     4621 atgttttaaa gcctcttcat agctaccaga ataatttaga gcatttgagg taagttcacc
     4681 aaaatctctc tttggtattt cactcttaaa caatcttaaa gtatttatac attggttttg
     4741 gtatccgatt attggtatat cataaggcat tgccattaca ggaaggtagt ttttatgaat
     4801 tacttttaaa tgactacctt ccttgattaa ttctacctca ccaccaaact tcaccattgt
     4861 tgcttcattt ggtcttacag tttcccatgc atatcgtcct tctgtaagcc agttatcagg
     4921 cacttctacc tgataaccat ttacaaattt ttgttcaaac agaccatatt tataccttat
     4981 tccatatcct tgaccactta tgttcaaaga tgccatagaa tctaaaaagc aagctgctaa
     5041 tctaccaagt ccaccatttc ctaaggctgg gtctacttca gcttcaatta aatcatccaa
     5101 gttgattcct aattcagata aaccttctct aacttcttct tcaatattta aatttaataa
     5161 attagatttt aactgtctcc ctattaaaaa ttctaaacta aaataataaa cttctttttt
     5221 cttatctaac tttgtagcaa cccatttttt tgctatctca tctttcaata gactacataa
     5281 tacatttaac aattgttcat cagtagcttc cttgatagat tgtgcatata agctatacat
     5341 ttttacttca aatttttgct tgaatttctt tttgcttatt gttaccaaaa aaaccactcc
     5401 tttaatcaca tatgtctcta tatgtttcta tatactcctt agctgatttt ttccaactat
     5461 tatctgtttt cattgcattt tcaactaatt taatccattt ttctttgtcc ttaaatactt
     5521 tgattgcatt tttcaaacag aaaaacatct catgagcatt gtaattttta aaactaaatc
     5581 cattgccttc tccagtaaat ttattataag gtgttactgt atctctaagc cctccagtct
     5641 ctctaactat tggtaaagaa ccatatctca tagctaacat ttgacctata ccacaaggct
     5701 caaataatga tggcattaag aacatatcac ttgcagcata tatctgttga gccaatgatg
     5761 cattaaaagt aattctagct gatactttat ctgaaaaatt agaatcgtaa tagttgaaca
     5821 ttgattgata ttgatgttct ccagttccta aaacgacaat ctgtaaatct tctctcataa
     5881 gttctggcat catgtatgct attaaatcaa atcctttttg agaaactaat ctcgaaacta
     5941 tacctatcaa cggaatagat gaatcttttt taagtcccaa tatatcttgt aatctcaatt
     6001 tattttctac ttttttgtca attgagtcta catcataatg aacaaatata tctttatctt
     6061 ttgcagggtc gtttaaatca taatctatac catttaatat accttttaat tttcctgact
     6121 cttttctaag taatccatct aattgttcac cataaaaaga tgtttgtatt tcatttgcat
     6181 aagtaggact aacagttata attttatctg caaaatttat accagctttc ataaaattta
     6241 ttgagccata atactctata tctctattat caaagtattt ttgtggtaag tccaatatat
     6301 catataaaac atgcttatca aatacacctt gatattggag attatgtata gtatacatag
     6361 tttttatatt tttatacccc tcaagggttg catatctttc ttttagaatt aatggtaaca
     6421 tccctgtatg ccaatcattt atatgtatta catctggata aaaatcaatt ctacttatag
     6481 cttctaaaac tgcatctgta aagaaagtat atctttctgc atcatctcca tatccataga
     6541 tactctttct ttcatcttct cttctaaaat aaaattcatt atctataaag taaaatttta
     6601 caccatccaa ttccatttct aaaagaccac agtattgatt tctccatcca acacgtacac
     6661 taaacacagc tatttctttt agttgatctt ttaaataact tggtatagtt gaatatttgg
     6721 gcattattac ccttacatct acaccttctt tttttaattc tttaggcaat gcataagaaa
     6781 catctcctaa tcctccagtt tttgcaaagg gccaacactc tgctgttaca taaaaaacct
     6841 tcattagtca ttcccccttc taaaaaattt atttatatat ttgtattctt ttccactaca
     6901 agaggatatt tttcatctcc tcttaattct ttcccatttg atacactcgt atttttatct
     6961 aatattgcat tatatagttt tgcatttgac tttattacac aattttgcat tactattgag
     7021 ttctctataa ctgttccttt ttcaacattt acctttctaa atatgatact atcttttaca
     7081 gtaccttcta ttaaacaacc agatgctata aagctatttt ctactttagc actatctgta
     7141 taaattgtag gtgattcatt cttttctttt gtaaatatct ttctttcaga gtataataat
     7201 tcatctgcaa cttctaaatt taataaatct ttgctcattt caaaatatga ttcaacagaa
     7261 tttacacatt ttagatatcc tgtatattca taagctccta ttttcatatt ttctgcttct
     7321 tcaataataa attcttctaa ataacaataa ctgcctttat ttacaatgtt atatatacat
     7381 tcaacaaaat cttcacgctt cataatatat gtctccatac atatgtttgc tctagggaat
     7441 ttgcctaaat tatttcctac atttattacc ctattgttac tatctaaatt tagtgttaag
     7501 ttaccagcaa aatctttatt tgcattattt acatttttat atattatagt tatatcatta
     7561 tgagattttt tatggtattc caatgcttga gaataattta aattgcatag catatagctt
     7621 ggagctatta aaatatattc ttcttcactc ttttctatat aatctagatt agctaagata
     7681 gtatatatat caccttctct atgtgagtaa gtatttctat acttagtgtt ttctggacta
     7741 aaaattgata ggccaccttt ttttctactt aaatcccaat ctttaccact acctatatgg
     7801 tctgtcaatg atctgtattt ttcttttgcg aatattccaa ctttagtgat tcctgaattt
     7861 atcatattag ataaagcaaa gtctattatt ctatatcttc cagctattgg tgtagatgct
     7921 attggtcttc catagttaag tttgtttatt gctgggtctc cttttttgtt taaatttatt
     7981 atccctaaac attcatttct catcttaaag acctccttct attaatacgc atctaggttc
     8041 gatattttca tactcagaaa ctacatttat ttcattattt acattcttta tctgaacatt
     8101 atcatttata gttgcacact cacctatcat agctttttct atagttacat tttcacctat
     8161 tacaacattt ggcattatta ctgaatcaat tactttactt cctttaccaa caacaactcc
     8221 atgtgataat acagagttgc ctacttcacc taaaattcta caaccatcag ctatcattga
     8281 tctatgaaca tttgcatttt tacctatata ttgtggaggc attgccatag tatttgtata
     8341 tatcttccat ttagggtctg ctaaatcaag tgtttctgga gattttatta tatccatatt
     8401 agcatcccat agactttgta tagttcctac atctctccag tatcctttaa atggataagc
     8461 atacattcca acattatctt ctaacatttt aggtattaaa ttttttccaa aatcatcata
     8521 atttatttct ggattttttt ctgcttcctt aaaataattt ctcaacattt tccaatcaaa
     8581 tatgtatact cccatagatg ccaaattgct cttaggttca cttggttttt cttcaaattc
     8641 atagatactt gaatcttcat ttgtattcat tatcccaaat cttgaagctt catcccaagg
     8701 aacttcaatc actgctattg tagctttaga gcgtttttcc ttatgaaatt ttaacatttt
     8761 gctataatcc atcttgtata tatggtcacc agataatatc aatacatatt ctggattata
     8821 ggtatctaca aagtccatat tttgatatat cgcatgtgct gtaccattat accaatttcc
     8881 ttctttttca ttcataaatg gttgtaaaac atagacacca ccatttattc tgtccaaatc
     8941 ccaatgactt cccatgccta tatgtgaatt taatatcaac ggtctatatt gagttaaaac
     9001 acctacagta tcaataccag agtttgaaca attacttaac acaaaatcta taattctata
     9061 cttacctcca aatgatacag ctggtttggc aattcttttt gtaaaaacac ctaatcttga
     9121 accttgccct cctgccaaaa tcatagctaa catctctttt ttcatttaat gacactcccc
     9181 tcatctagta attaatattt ataattgcat ttttactttt ataaacttta tcagtcaata
     9241 aatgccgtgt ataaatgttg cttaaataat accttaataa aaataaaata atatcaacta
     9301 tacaattgat ataatgtata ccccataatg aacttaccga cgtatacatt attttccata
     9361 agcactatag tatttattaa tattcaatta tcggttaaaa aataatacaa attttatttt
     9421 ataaatataa aaacattttt tataaatccc ttgtgtcata taaacatgct tattttttaa
     9481 tttgttaaat tcgtaatagc aaaatataat ctaatatttg ttaaataatt atccaaagaa
     9541 aatcaagtaa ttattaatca actttatttt ttaattgata cttctttgtc tataatttaa
     9601 acaaaaggat ataaactttt aaattattta ttagttttct tagtattaat gtttatattt
     9661 ttataaatat atattaataa tataaataga ctaactaaaa ccatgcttat ttgctatatg
     9721 ttttatataa tattatataa cactttttac attagtaaag gataaattgt ataaagattc
     9781 tgttaattca tttttataat ttcatctttc taataaaaac aaagaagaag acttaaaaat
     9841 ttataagtcc tcttcttttc aacgtgcatt tttgtgctat atttaattaa ttcgaccttt
     9901 aatttccata tccttcctat gataacaaaa ctcttttatt tttttaatga ttcattatca
     9961 tttaaaactt cttttatatc aagtacttct ttgttatcat cactcataac ttcctttatt
    10021 cctttgaatg ttcctaggaa attaactgct tctgaaggta aaaccagttt tgttgactta
    10081 ccttcagcca ttttttcaag agcttccata gattttaatg ctagaatatt attgtctata
    10141 tcagcctctt tcattgctga aaaaactaat tttaaacctt ctgctgttgc catttgagtt
    10201 ctttttatca tttcagcctc accttgtgcc ttagcaatag cggtctgtct tatagcttca
    10261 gcttcacctt ctgccactaa tatagcagat tctttttcac cttctgctac acgtaccata
    10321 gcttcttttt tggcttctgc tgttaatatc gcagattgtt tttcaccttc tgcttgaagt
    10381 attgcagctg acttattacc ttctgcttga agtattgcct ctcttctttc tctttctgct
    10441 ctcatttgtt tttccatcgc aacttgaata tcctgaggag gcattatatt ttttaactct
    10501 actctattta cttttatacc ccatttatct gttgcttcat caaggatagt tctcattttt
    10561 acatttatta tatctcttga tgttaatgtt tcatctaaat caagttcacc aattatattt
    10621 cttagtgttg tagctgttaa attttcaata gcagctattg gattagctat ctcaaacaca
    10681 tatctaactg gatctgtaac tttataatat acaactgtat caatctgcat agttacatta
    10741 tctttagtta taactggttg aggtggaaaa tctattacaa tttctctaag gtcaattaca
    10801 tatgccattt tatctaaaaa tggtattaag aaatgtacac ctgtttcagc cactttttga
    10861 aacttaccaa gtctcattat tatacctact ttagattgtt ttataactct tatacacgtt
    10921 aaacttattg ctacgactac tactattaat acaatactta aaacaatttt agtacccata
    10981 ctataaatcc tcctttattc tattggtttg tcacattaac tgtggcgtct acttttttaa
    11041 caactaattt taccccttca atttttaata cttctactgt agaacctttt tctatctttt
    11101 caccatctat agaaatagcc gtccatgttt catgttcaac tacaactaga cctgtgttat
    11161 ttggagaaat ctcttctgta acaatacctt ttttagacat cattgcttgt aaattagtgt
    11221 tagatttata gcctttatct ttttttacta tttttttagt agctacgact aacattgcta
    11281 tagaaattac tgcaaatatt aaaatttgta agaaaatact ctctataaag ctacttaaaa
    11341 atataagtat tacagctcct acactaaacc atatcaatgt taagcttggt gttaacattt
    11401 ctgctattcc aaataagact gctactatca accatattaa cttcatacta tctaccctct
    11461 tttcatatga attataaaac cctatataaa ttatatgttt atacaatgtt tttgaatata
    11521 aatttaaact acaaatcaat agaataaact aatctaagat taatttaata gtattatatc
    11581 aacatacttt gttaaaattg ttacaaagta gtctgaacct cccatgacta aagtcacagg
    11641 gttcctgctt catagaattt tgcatactaa catatgtctt actaattctc cacaggctat
    11701 ccccgtagtt cctacggttc ttacataagt tatttatttt gaatcgttat tagtcttaat
    11761 ccttctttta atatatttat acttgcattt at
//
LOCUS       DAGQWR010000129        11671 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.129, whole genome shotgun sequence.
ACCESSION   DAGQWR010000129 DAGQWR010000000
VERSION     DAGQWR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.129"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(181..855)
                     /locus_tag="MIW95_003048"
     CDS             complement(181..855)
                     /locus_tag="MIW95_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3152356.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFMDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIDILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYII"
     gene            complement(1007..2914)
                     /locus_tag="MIW95_003049"
     CDS             complement(1007..2914)
                     /locus_tag="MIW95_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY3152357.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSLIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEVKTKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMNDLSGIYILSLVICLIIFVIYGAVS
                     VNKFKSVVYKN"
     gene            complement(2915..3667)
                     /locus_tag="MIW95_003050"
     CDS             complement(2915..3667)
                     /locus_tag="MIW95_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152358.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPAVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(3985..5139)
                     /locus_tag="MIW95_003051"
     CDS             complement(3985..5139)
                     /locus_tag="MIW95_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3152359.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMILSLFGCLAYGLIVLLNYLQE"
     gene            complement(5142..5609)
                     /locus_tag="MIW95_003052"
     CDS             complement(5142..5609)
                     /locus_tag="MIW95_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY3152360.1"
                     /translation="MFFKRKKEASTINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(5623..6111)
                     /locus_tag="MIW95_003053"
     CDS             complement(5623..6111)
                     /locus_tag="MIW95_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152361.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(6125..7456)
                     /locus_tag="MIW95_003054"
     CDS             complement(6125..7456)
                     /locus_tag="MIW95_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBY3152362.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARILGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMIEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(7470..9035)
                     /locus_tag="MIW95_003055"
     CDS             complement(7470..9035)
                     /locus_tag="MIW95_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY3152363.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFLFTFLIKKFDMKTPGREEDNEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKVVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(9204..9989)
                     /locus_tag="MIW95_003056"
     CDS             complement(9204..9989)
                     /locus_tag="MIW95_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY3152364.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(10156..11355)
                     /locus_tag="MIW95_003057"
     CDS             complement(10156..11355)
                     /locus_tag="MIW95_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBY3152365.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
BASE COUNT         4039 a         1966 c         1144 g         4522 t
ORIGIN      
        1 ttatataatc tataacaaaa aataccatta ttataaacag cactaaaaaa tctaagtata
       61 ttaattctga atatctattt tttaaaatat ctatagcaaa taaatagata tttattatac
      121 taactacaac taaaggtaga gcaatatact tagacctacc ttttaaatat tttactagca
      181 ttatattata tatcctatcc ccttcttagt ttttataaaa tcatcaaaac ctagttcttt
      241 aactttactt ctcaatctat tcatatttac agtaagtgca ttgtctgtca caaactcatc
      301 attatcccat agagtcatca taagactttc ccttgataca accctattct tattttttaa
      361 taataatgtt aaaattttta tttcgttttt agtcaactct atacttttat ccttatactt
      421 atgttttata atccctttcc ccacatcaaa catcaaatct tcacaataca aaatactatt
      481 agaatcacta taattataga ctcttcttat tataccattt attttggtaa ccaatatgtc
      541 tatagaaaat ggtttggtaa tataatcatc tgctccatta tttataccca ttattaaatc
      601 catatcagaa tctcttgatg ataaaaatat taatggtaca tttgaaatag ctcttatttt
      661 ttcgcaccag taaaatccat tatataatgg taaatttaca tctaaaagta ctacatctgg
      721 ttttttatcc ataaattcat ctaatactac ctcaaaatca tcaattaatt ctactttaaa
      781 tccccatttt tcaagtgcta acgcaatctc ttttgataaa tcaatatcgt cctctactaa
      841 taaaattcta tacatttgta tattctccat tatcctaata tttcactatg ccatgaatta
      901 ttaatatcat aacatatttt atataaattt attacccaaa ttatttagtt aatcaacaag
      961 gagactctta ttacaagaat ctcctttctc tttattaata tatcaactaa tttttataca
     1021 ctacactctt aaacttatta acagatactg ctccatatat tacgaatatt atcaaacata
     1081 tcactaaact tagtatatat ataccagata aatcgttcat caataaatct cctgctaatc
     1141 tcaatgcaaa ataactagat atagttgcac ttaacattgg caaagtataa aatatagcta
     1201 cttctttaag tatagctcta tttatatctt tttctgtagt acctattttt aataaaatct
     1261 tgtatttctc tttatcttca tatgcatcac tatatatttt catatatatt atacttccag
     1321 ctacaattgc aaagactaag aataaaaaca caagcactac atatgcaaac ttcatccatt
     1381 gactctcacc ttgagcttta tatccattct gaatatatcc attttttaaa cttaaattct
     1441 tttcaatatt gttgaacatt acttggctat tttcttcttg aaataccttt gcaccataaa
     1501 aatatacttg ttgaccctta tctcttagtt taaaatatgt atcatcattt accactataa
     1561 tactttgatt aagtcctgtc cctaatactg gaacttttac ttctcccctt gaaatattat
     1621 ataactgacc atcaagtttt gtcttgactt cttttgtagc tattgatgct atcgtttgag
     1681 gactttttag ctctattaca ttatttccac taactaatct ttcaccatac tcttttaatt
     1741 ctttatcatt gccattcact ttaagagtat tacaaaaatc actatatttc attatagtat
     1801 ctgttgtatc ataagcttct tttatatctg ttttctttaa gattgttagt tctggattta
     1861 cggagtactt tatcttatta gtatctttta ttatgttctc tatttgtttt ttattcactt
     1921 catctttatc cattgaaact atgctaatag tgtacacatt tctttgttct tgggcattct
     1981 tatgtatatg cttcatagca actgaagcac caagagttga tattgttgta gttataatta
     2041 tagctattat agcataagtt ttatagtttt tatttaatcg gtagtttata ttgtttatcg
     2101 caattatatt atttccatta aataatattt ttttattttt gattataata tcaaatataa
     2161 caggcataac tgatttgaat aagccataag tacctactat tattatacct ataacaggta
     2221 gtacactagt tatatctcca gttcttatat tcgtagaaag atagtatcca tatcctatta
     2281 ctataagtga tactatagct aatatatata tacctattcc tacctttggt attttttctt
     2341 gttttttaga tatattgatt agatttatta cactacttct acatattgtt ataaaacctt
     2401 ttacagccat tattataaat atagaaataa agattaaagt agcatatagt atagcttcta
     2461 tgctaagtcc attagatacc tttatattaa atccagataa ttttattatc actacttgaa
     2521 aaaatctaga tgttattatc cctataatta ttcctgttat acaagaactt attcctataa
     2581 gcatattttc tacaaaatat atttttccta tagtatataa atctaatccc ataaaggaca
     2641 atattccaat ttctttagac ctattcttaa aaaatatatt tgttgcatac catataaaga
     2701 aaaataaaaa tactgatagt atcattatta cagctactaa cactgatagt gtaaattttt
     2761 tattttcctc ttgtaacaca tttatagcat ctccatatct taataattca aaattagtca
     2821 gcacaactac tgaaaaaacc atagatataa aaaatgctag ataagttctt atgttactct
     2881 taaaattatt ataagcaatt ccaagtatac tcacttacat ctcacctccc atagaggcta
     2941 gcatatccat aatttttcca taaaattctt ttctactatt tcctttgttt aatttacact
     3001 ttatatttcc atcatttatc atatatactt catttgaata acttgccgtt agagggtcat
     3061 gtgtaatcat tataatagta gcgttgtttt ctctgtttat tttttctaat gattctaaaa
     3121 gttcatatgc tgatttagag tccaaagcac ctgttggttc atcagcaaat atgactgctg
     3181 gatttgtaat taatgaccta gctgctgcgc ctctttgttt ttgtcctcct gataactgat
     3241 atggatattt atctaaatga ttttctaatc caaatttttt agctatagaa aataccttag
     3301 cttctatctc cttgcttttc ttttttccta atgctagagg aagtgctata ttgtccatta
     3361 gtttcatatt gtctaataga ttatagtctt gaaatataaa ccctattttt tctcttctta
     3421 attttgatag ttcattattt tttacttttg atatattaat tccatctagt attatttctc
     3481 ctgaagtttg tttgtcaagt gtagacatta aatttagcaa tgttgtcttt cctgcaccag
     3541 atggacccat tattgctata aaatcacctt cattgactgt aagagatata ttttttaaca
     3601 catttgtttt aaatcctttt attccataat cttttgatat attttttatt tttaatattt
     3661 ctttcataat aatagcctcc agttgctaat tttttaatat atatttattt taaaggtttt
     3721 atataaataa atccttaagt aaatattaca ttttaactgt tagcattaca cttttgtaac
     3781 atatggaaga ctattataat tatagtggaa atatcttaaa ataaaagcat gattcacttt
     3841 tttgtataaa caaagaaaag aagcccctaa gaagcctcta atctatacaa taattcttct
     3901 ctataaaaaa ccaaaaatac aaccttgatt gtggcaaata caggtactat aaacaagtga
     3961 tattactact atatattaaa acaattattc ttgaagataa ttcaataaga ctatcaagcc
     4021 ataagctaag catccaaaaa gacttaatat cattaatgta taattatacc caatattaat
     4081 actcaaaaaa ccagcaacag ttggaataat cagagatgaa cctcccataa atatacttaa
     4141 agctattaat tggctactac ttctaagttt tttaggagaa acttcaaata ttaatctttt
     4201 tgaagtaatt aaaattaaag gagtagaaaa cacttgaaaa acacacagta taataatgat
     4261 atttgaatca tttgttaaag caaataatag aaactgaact gttatcataa tagcacttaa
     4321 ttttaataag tttaaactgc tatatttttt taataaataa gaaccagcca gatatgttgg
     4381 tatctctaat aatgactgta aagaccactt taaagatatc tgagaatttg tagcatttaa
     4441 agctatcatt ttatctatta cagtacagtt atttgctacc accattgagt ataataagaa
     4501 taaaatacat actagtaaga tatatttttt atcttttaat aattcaataa tatctttatt
     4561 agttacattt attttcttat taatcttttc aatatcaggt aataatagta tatttataat
     4621 aagtaatact acaattaaac caatcgctac agataatcca aaatatgaga atctaagaat
     4681 caagtaagat gctaccatac ttccaatagc ccatccaatc gacccaaaag tcttaataaa
     4741 tgacaaataa ttattaatat tatctccaca acctattatc catgtatcat ataaactaca
     4801 gcaagtattt aataatcctc ctgataaagc aacaagtaat aagtatatta taaaagttgc
     4861 agattcagta gaaaacataa taactgatgc aattccataa actgataaac taattaaaac
     4921 aatttttttc attgtttgat acttatcagc taatactcca aataataatt gaaataatat
     4981 attggttatt gcatagctcg ataatataat tcctctttca aacgctgagt aacctaattt
     5041 tgataagaaa ggtgtgaatt ttgttgaagc aaaggcaaaa ataatatatg agaaaaaata
     5101 gaatatgata aatttaaatt taatcgcttt attaatcata atcaatcaaa attgataact
     5161 ttaataccac tatttccttc ctgatttaag tttgaaagat gatattttac cgtattagga
     5221 ttctctacaa taactaaagg aatatataaa gtattttcaa gtaacttatt atcaatttct
     5281 actaactttt cccccatatt aacttcttga ccttgtttaa cttctaattt aaagttttct
     5341 ttttttgttg ttgcactatc caatccaata tgaataagaa tttgaacatc attttgcaat
     5401 ttaattccta tagcatggtt agtttgagca ataaaactta ttactccttt agtcggtgat
     5461 ttaagaatat tgtcctttgc tatcatagca aatccatctc ccattacctt agttgaaaat
     5521 actttatcag gaatttctcc aagattaatt atttttccat caatgtatga attaattgta
     5581 cttgcttctt tttttctttt aaagaacatt atagacactc ctctacataa ttaactttat
     5641 tctaattatc attacaaata acgatattaa gcatactgca attgaggaaa aaagagcctt
     5701 atagctcttt ccccattgta gttctttaat actgcttatg atttgtttaa ttccaacaaa
     5761 tttataaata taggtaaaca tgataacaat agttattgtg taaatttgga atatctgccc
     5821 taataagact aaatagaaag caatcatgtt ttcttttatc tctttattct tttttatatt
     5881 gtaaataaca tatgcactgt atactgcact aaagttagag attaaattta aacttgcatt
     5941 aacatcattg tttaaaagtt gtgaaaatga aatgtttgta aataatgatg tcaattctaa
     6001 atacactata tataaaacag gtaatattag aaaacaaacc ataaccactg aaagaaatat
     6061 attttcatct tttaatactg ctgttatatt ttttatttta tttgttatca tgtttttgtt
     6121 ctccttattt aaaagttggc cagaaatcct tattagccac aatcatttca tctaagattt
     6181 cttttgctac ttttgcactt ggaacagttt tactcatagt aaacgactgc cataattttt
     6241 gataactttt ttctatccat gcttctacag ctaatttttc agaagcaact tgttgttcca
     6301 ttaaaccttt ttggaaagtt ggaattttac ctatagacat aggttcaacc atatcttttc
     6361 caacaaggca aggaatttca atcattgcat catctgcaaa attttcaata gctccattat
     6421 taggaacaat cagtaaaaat ctttcctgag tattaaatgc taatgcacaa gctaattcaa
     6481 caataaattg agaatgttca cttgcatcaa accatgtatc ccttgctgtg ccatttttta
     6541 taattctttc acattcttcg aatgtatcct tttctcttct atccataatt tcatttgctc
     6601 ttgtataatt tggattagca tgttcaacag tttcatcagc cattaaataa tattttaaat
     6661 aagtattagg taatgtagtc ggttctattt ctaataagtc ctttgctgct ctcatagttt
     6721 ctaaccaact agattctttg tgttgacctt gaggtgtaaa ttcctcatat ccttttttag
     6781 cacaatattc ctgtaattta ggcattaaat catttccttc tttgtcctta atacttgtcc
     6841 accacccgaa atgattcaat ccataatatc taacatcaaa gtctttacga gaatttaaac
     6901 ctaaaattct agcaaatatt tcctctaaac atactggcat atcacaaata ttaataattc
     6961 ttgaatttgg tcttaataca cgacatgctt ctgcaactat agctgctgga tttgaataat
     7021 taagcatcca agcatttgga gaatattttt ccatataatc aatcatttcg attattggac
     7081 ctattgttct taatccatag gcaattccac ctgccccaca agtttcttga ccaacaaccc
     7141 catattttaa tggaattttt tcatcttgtt cacgtaaagc atataaacca acacgaattt
     7201 gtgccattac aaaatcaata tcagtaaatg cttcttctgg atttgttgta taactaaatt
     7261 taatttctgg atttttctct ctaacaatta ttgcacatgc ttcagcaact tttctttgtc
     7321 tctcatcatt attatcatat aatttaattg attcaatagg aaatttagca acactttcaa
     7381 ttaacattgt tacaattcca gcagtataag tactaccccc accagcaatt agtatcctct
     7441 gtctttttct ttccatcttt ccttctcctt tataataaat tttcaaattc ttctcttaca
     7501 cttggtactg aaagcccaat aataatctga atagcttttc cattatgcac taaaccatgt
     7561 gcacctacta ccttaaaatc ttcttcttta gaaataattg agtcatcctt caaggttaat
     7621 ctcaacctcg tagcacagtt tgtaacatct acaacatttt ctgctccacc aactaatttt
     7681 atgtattcag atgcttgttg agaggcttta ctcaatttat ttcctttttc atctttagtt
     7741 tctaagtatt catttttagt atatagtttt gtttcttcat tatcttcctc tctaccagga
     7801 gttttcatat caaacttctt aattaagaaa gtaaataaaa agaaccagat aaatgaaaat
     7861 ataatcccta caacaacttg aacaacatat accttccagt gatacattcc aagaggaagc
     7921 caatttaata caacccagtt aataattcct ccaccaaaat caccaacaac tcctaaggca
     7981 aacgaaatcg tttgcaagca tgcatctaaa aatgcatgta ctaggaataa aagtggtgct
     8041 acaaataaaa atgtaaattc taacggctca gtaattccag ttaatactgc agtaagagta
     8101 gcaggaatca ttaaacctaa tacttttttc tttttttctg gctttgcagt tacatagaaa
     8161 gcaccaaata cacctaatga accaaacacc ttcgataatc cagataatga aaatccgatt
     8221 ggatacattt cctttagagt cttagctgaa gtttgaaagt cctgaatatg agtagcccaa
     8281 tatactgatg taccacctgg aacaacaaca ctatcataac aaattggtgc atatacaaaa
     8341 tgatgtaatc cagttggtat taaaatcttt tgtaagaatg cataacacca aactcctata
     8401 tttccactat ttataaaaaa cgtttgcaaa tgtctaataa cattttgaat atgaggccaa
     8461 ataaaacaag ttaataatgc aactggtatc ataacaaaga aacaaattgc aactacaagt
     8521 gatgagcctc taaataatcc aataaaatct ggtagttctt tatcataaaa tctattatga
     8581 atataaatga caatacctga tattaacaaa gcaccaacca taccagtatc taatgtttta
     8641 ataccagcaa tgcttacaag tccacttcca gcacttattt cagctgcgaa atcaactcca
     8701 aagctttttc cccaaaaact taacatagaa cctacaaaat aattaaaagt caaatacgtt
     8761 gctaaagcct ccatacaagc tcttgcttgt tgcttttttg caagtgctat tggtaaagaa
     8821 atagcaaata ataatggtaa ttgattaaat accatccaag ctccactaga tattacaccc
     8881 caaaattggt accaaatatt ttctggtgta gcaagtgaac ccataattga ctgattttga
     8941 aatacaattg agaatccaac cattatcccg gcaaaagcaa ataatagtgt tggtgtaaac
     9001 attgcccctc caaaacgctg tattttcttc atcatataaa ttactcccct cctcatattt
     9061 ttagtttatc tttggcctta gatatctatt attttaacag gttaataaat atatacaatc
     9121 tttagggtac atttggagct tattcacagt caaataagtt tttgcacatt tttatgttca
     9181 aattctcata aaaaaaagaa tgactacaat ttgcaatcaa tcccttctgt aatcattcta
     9241 tgccttttat aaatactata tttaataaat aataactcaa tcaatataaa atataacatt
     9301 gtcaatttat aactagggtg catttgtaaa aatgagatat tagtcgctga aatataaatt
     9361 gattcatcac ttaaatcagt caatgtatta tttttaaact ctgtaattga aattatttta
     9421 gttcctatta attttaatct tctagcaata ttaagtgtat tctcattctc accacttagg
     9481 gatataataa tcataacatc atttttactg acaatattgt aaaaagaatc atcaaatgtt
     9541 cttccctcaa aatcatatat tatttcttga catgataaga acattctctt tatctgtttt
     9601 gcaaccacat tttgaatatc acctgaacca taagcgtata ttgttcttga ttcataaaga
     9661 agtttacaca atgcatcata atctttattt ttttgatctt caacatactt aattattgaa
     9721 ttacatgcaa tatcaaatga ctgattttct atgacatgaa ccttttctga ttcccagctt
     9781 aataatactt taaactcact gaaacctttt aaatctaatt ttttaacaaa tcttgtaata
     9841 gttgcacgag acacattaca tttcatagca agttcattta ttgacatctt actacaatgt
     9901 tttttatttg cagttatata tttccatatt aataaatctg tatcattcaa tttattataa
     9961 tttaaaaaaa tcatttcttc taatctcata aactattatc ccctttaatt aaaaatcgtc
    10021 ataaatatta taccaatatt caacattaaa ttacagcgta ttcttatatt tactatttag
    10081 cccataaaat tgactataaa aaatactgct ataattgatg tttaaaaaaa tgtagtttta
    10141 attttattct tttatctaat aattaatatt ttcaagttct agtgcttttc ttatgcaatt
    10201 atcatcttta aaatcagcag tccttttaca atcttttact ttaaattctg gaatagtttt
    10261 aaattcctca tcacatattc cattcttatt taaatacata caacttgcca ttctaactat
    10321 tagaccacta ttttttaaat taaacaatac agggtctata caccctccat ctccgccagt
    10381 agtttcacca actaaagtcg caaatttagt ttctttacaa aacattgcaa atgactctga
    10441 tgatgaatat atttttctat caacaagtaa gtatatatta cccttaaatt ttgaatcatc
    10501 tgtagatttt atagtatagg aagtatcttc aaaatcatta aaatctttta taatttcttt
    10561 aggtgcattt tcctttacac ttgctggtaa attttctaca ggatttaatt ttatatttct
    10621 tgcatttgta tattttttta ctatttcatt attatttcta taaattctat aaccatttat
    10681 tttgacatca ttttttatga gtttagatac gattccctgc caatacctat cacttccacc
    10741 taaattacct cttatatcaa taattaaagc tttatgtttt tctaaagtgt taatataatc
    10801 tccaattatt tttaaatcat tatttataga gccattcttt gatgacattg atggtaaata
    10861 catatatcca atctgaccct taataacatc ctttaaaatt aattcttttt ttacaaaaat
    10921 atcatctggc atttttattt taagattcat agagtcatat ctatccacaa cttttttatc
    10981 attaagaaaa tcataccaat cattattaga gtatgatttc ttaaaaagtt catatctttt
    11041 cttattatct attacttctg tgtgtttatt atttaaatca gataatatag atgacagttc
    11101 ttctataaat tcttcatcat tttcagtgtt ttttattcgt tttgtatatt catccttatt
    11161 tgctaaccaa tcaatattat taagtctctt atttacacct agatatggat atcctgtctt
    11221 tacaacatta tacacataat taaagtcatc caccttttcc tcttcagaca actgattctt
    11281 ttgagaataa gtagaattac ctaataagat tataaatgct aaaataatta ctaaaaataa
    11341 tactttcttt ttcatgtaat tctcatccac tttcagattt tatattttaa tacattgtat
    11401 cattatttta ttatcaccta aacagttaaa agaaattctt gaagatatat tccactatta
    11461 ttctatttaa aaatttttat ttgtattttc aaaattattt catgaaagtt ataaaataac
    11521 aattatattc aaatatctat tttactgcta tatcctgaaa gtaaattctt atcagtgata
    11581 tactaaaaaa gatatgctaa aaaatttatc cattataatg acttaaattt agatacttta
    11641 ctgattttta tttattagtt gagaaaggta t
//
LOCUS       DAGQWR010000130        11537 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.130, whole genome shotgun sequence.
ACCESSION   DAGQWR010000130 DAGQWR010000000
VERSION     DAGQWR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.130"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            531..1196
                     /locus_tag="MIW95_003058"
     CDS             531..1196
                     /locus_tag="MIW95_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBY3152366.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            1218..2150
                     /locus_tag="MIW95_003059"
     CDS             1218..2150
                     /locus_tag="MIW95_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBY3152367.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            2171..2812
                     /gene="pcp"
                     /locus_tag="MIW95_003060"
     CDS             2171..2812
                     /gene="pcp"
                     /locus_tag="MIW95_003060"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBY3152368.1"
                     /translation="MKILLTGFDPFGGELINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIK
                     GGFIHIPYTTSQVINKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(2931..3263)
                     /locus_tag="MIW95_003061"
     CDS             complement(2931..3263)
                     /locus_tag="MIW95_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3152369.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            3529..4149
                     /locus_tag="MIW95_003062"
     CDS             3529..4149
                     /locus_tag="MIW95_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY3152370.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKEAI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(4474..5298)
                     /locus_tag="MIW95_003063"
     CDS             complement(4474..5298)
                     /locus_tag="MIW95_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBY3152371.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     TLFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(5309..6712)
                     /locus_tag="MIW95_003064"
     CDS             complement(5309..6712)
                     /locus_tag="MIW95_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3152372.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(6716..7441)
                     /locus_tag="MIW95_003065"
     CDS             complement(6716..7441)
                     /locus_tag="MIW95_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3152373.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDVQNESKNLNKNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(7553..8326)
                     /locus_tag="MIW95_003066"
     CDS             complement(7553..8326)
                     /locus_tag="MIW95_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152374.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFLGFLLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVRYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(8332..9219)
                     /locus_tag="MIW95_003067"
     CDS             complement(8332..9219)
                     /locus_tag="MIW95_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152375.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDANYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVFDEP
                     TAGIDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSGDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(9233..10255)
                     /locus_tag="MIW95_003068"
     CDS             complement(9233..10255)
                     /locus_tag="MIW95_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152376.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETERYYID
                     KEKGCIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            10830..11006
                     /locus_tag="MIW95_003069"
     CDS             10830..11006
                     /locus_tag="MIW95_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152377.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         4162 a         1720 c         1289 g         4366 t
ORIGIN      
        1 tctatgataa attcttttat ttataagtaa tattgctcaa aatagtcttt aaactttatc
       61 aaaattttat ttttcttagt gaaatctaat gaaactagct cttaatatat gctttattaa
      121 tcataacaaa aagagagtgt ctaatgaact aaaaagttca ttagacactc tctttttgta
      181 tgaaatcaaa tatattttta tcatttcaaa agagaaaaat acacttgtct ctatgtttga
      241 cacattctct ttttataata taatatctaa tttaaaacct atttcaaata ttgacatata
      301 cttaaaccat agacttacaa aatcaattca tattagctta aaatataatc tttgttactc
      361 aaattcctat acacaaccat tatttacttt gatactttaa aacatcttcc ctatttttta
      421 ttaaatttta taataatagt cctattaacg caaatactat aaaaagagtt taactttatc
      481 cattaaataa atttaaatta aaacatatag aaatggaagg agaacaattt atgataaaac
      541 taatcggtat attgatagtt gtaattggat ttatacttaa aattgacaca ttatttactg
      601 tattattagc aggagtggca acaggaattg ttgcaggatt agattttaat caaattttta
      661 caattttagg agattctttt gtctcaaaca gaggtgtatc attattcata ttaacactgc
      721 cagtaatcgg tgtattagaa agatatggtt taaaacaaag agctgtttct ttaatagaaa
      781 aattaaaaag acttacaaca ggaaaagtac ttactatata catgatagct agacaaatag
      841 ctggtgctct atcaataaga atgagtggac atcctcaatt tgtaagacca cttgttaatc
      901 ctatggcaca agctgctggt ttaagtaata gtgatgaact aaaagaatcg gatgaagaag
      961 ccataaaggc attatctgct gcatcagaaa actatggaaa tttttatggt caaaacttat
     1021 ttgcaggtag tagtggagta ttacttatag catctactct aactcaattt ggatacaatg
     1081 taactggact taatattgta aaggctagta ctattatggc agtaatagca cttgtagtgg
     1141 ctacaatcca gtttacctta tatgataaaa aattaaacaa aagtcataaa aaatagtttt
     1201 atttaggagg aataaaaatg caaaatttta taaacatatc attggaaatc ttttacgcac
     1261 taatgggatt tttaatgatt gttatagcat ataagtcttt tactaccata aataacaata
     1321 agaaatatgg tacatcatta ttttggatac ttatatcact gccattcata tttggtagat
     1381 tgattccagc caatataata ggaataatat taatattatc tagtttatta acactttcta
     1441 aacaagttgt atttgcaaag tatgaagaac cagatgaaaa ttttggaaaa gaacaagcag
     1501 acaaacttaa aaataaaata tttataccat cattaatatt agcatttgct gcagtagtag
     1561 tagctatgag cctttctaat tttgataatt cttctcaatt tgcaataggt gtgggttcaa
     1621 taattgcatt aatatcagcc cttataataa ctaaagcaaa acctgctaca tcagttcaag
     1681 atggctcaag attacttcaa caaatgggac ctgctagtat gttaccacaa cttttagttg
     1741 cattaggagc tttatttact caggctggag tcggcgaagt tatttcaacc atgatatctg
     1801 gtgtagtccc tgctgatagc agattatttg gtgttatagc ttatgtatta ggtatggtta
     1861 tatttaccat gataatggga aatgcatttg ccgcattttc agtcataacg gcaggaattg
     1921 gtattccttt tgtattgtct caaggtggaa atcctgctat aattggagca ttagcattaa
     1981 cagcaggata ttgtggaact ctacttactc caatggctgc taattttaat atagttccag
     2041 cagccttgct agaatgtaaa aatgattata ttgttataaa atatcaagct ccagttgctt
     2101 tggtattaat tatagcccac atattagtaa tgtacttttt aggattttaa tataattagg
     2161 aggtaaaata atgaaaattt tgttaacagg atttgaccca tttgggggag aactaataaa
     2221 cccagctcaa gaagcagttg aaagggtcaa taataatata aacggtgcag aaatcataaa
     2281 gataactata ccaacagtta tgacaaaatc tgttgaagct atagataaag ctattcaaga
     2341 gcacaatcca gatatagtaa tatcagttgg acaagctgga ggtagatttg acattacacc
     2401 tgagagagtt gctataaata tagatgattt tagaataaaa gataatgaag gtaaccaggt
     2461 tatagatact attataaaag aagatggtga acctgcatat ttttcaaaat tacctgtaaa
     2521 agccatggta aaacatatga atgaaaataa aattccagct agtgtttcaa acactgcagg
     2581 aacattcgtt tgtaatcatg tgatgtatgg aatcctttat atgatagaca agaaatatcc
     2641 taatataaag ggtggattta tacacatccc atatacaact tcacaggtaa taaataaaaa
     2701 aaatacacca ttcatgtctt tagaagaaat agtaaaaggt ttagaattag ctatagaagc
     2761 ttgtattata tacaaagaag atgtaaaaga aattggtggt gaaatttcat aataaatttt
     2821 ttagtatttt ataaattaaa aatactaact attaatttaa atatagactg tgagtagatt
     2881 attcaaacaa ttttactcac agtcttttta tggcatctac agatacaaaa ttaaggtcta
     2941 ttcctctctc cccactcaca cattgcatct aaaattggaa tcagagattt tcccctttct
     3001 gataaactat attctacctt aggaggtatt tgaggatatt cagtacgaat aatcaagtta
     3061 tctgcttcta attcttttaa tgataaactc aaagttttat aggaaatagt atttatataa
     3121 cgttttaatg cattatatct gacaacttca aattctacaa gacagtaaag aattatcatt
     3181 ttatatttac cactaatcag tgaaagtgta taactaaatc ctgtttcact taaatctgta
     3241 tttgatatac agtctatact catatattac actctccttt tggttagtat tgaacaaaaa
     3301 tgtgcgtact tgtttttaga ataatttata ataaaattat agtgtaagaa taattgaaag
     3361 tcaattttat aataggaggt gtatttacta aaaaaagagt tattttaaat aggattctaa
     3421 gattagacat ttctatataa attgataata ttcatgaaaa tcctatttta taaaaaataa
     3481 tatctcttag aaaatctatt aaataaaatt cattggagga aatttataat gaaagtttta
     3541 ttgataaacg gtagcccaaa ccaatatgga tgtacttata cagcactaaa tgagattact
     3601 aaagtattga gtaaacacga tattaaaaca gaaatactgt atttaggtaa agaagcaatc
     3661 ccaggatgta ttagttgcgc atcctgtttt gaaactggaa aatgtattcg aaatgataaa
     3721 gttaatgaat tgattgaaga tctcgataat attgatggta ttattattgg ttctcctgtt
     3781 tacttttcta gtgcaactgg tcaattaact tcttttcttg accgtctatt tttcatagct
     3841 ggaagtcgca tggctacaaa attaggtgct tcagttgtgt catgtagacg tggtggtgca
     3901 agtgcaacat ttgaccaact aaataaatat ttttcaatca gcaatatgcc tattgtatca
     3961 tcacagtatt ggaatcaggt acatggcttt actccagagg atgttatgaa agatgaagaa
     4021 ggattacaga ctatgcgaac tctaggagaa aacatggcat ggcttcttaa gtgtatatca
     4081 gcaggtaaaa aagctggaat taaagaacca caatatgaag aaacaattat gactaatttt
     4141 ataaaataaa taaaaaatgc actaatatta attaaaacaa aacactaatt tataaataaa
     4201 ataagtataa atataggata aaaaacacta aaacagaggt attgaatttt atatcaaacc
     4261 tctattttat tattacatta ataaccaaac catatataac atcatatatt tctaaataaa
     4321 ttataatatt atattctaat gttattgaaa ttacaataac tgaatttctg ctcataaatt
     4381 taaatattct acaaatctca tctaaataac aattttaata gacctaaaac aatcctcata
     4441 ttgttcattt tgtattaaaa atatcactta caatcaaaaa ccatttatta agtatattcc
     4501 taagatatac aatatatata catggattgg atagtataaa taaaataaat acttcatact
     4561 cttccctttt tctccattgt aaagtaacat taaaggtaga gaagcaatca tcatccattg
     4621 gtaattttgt aaaatagaat tttttaaact ataatctcca tttgacattg ttgtaaatat
     4681 aaatatacac atcaaaacat aacaaatact caaactgaac tttttacctc tacacaaata
     4741 aaaaataatc ccaagtataa tccaaattgg tccaccttct actaaaaatg gtactggtac
     4801 caaaatcata aataaagtaa taactctgtt agtgacttta aaaagtaaag ctactgtaaa
     4861 tattcctaaa attataggaa tgattatcat caataatcct ataataaaat atttaatttc
     4921 tttctctttt ctaaatttct ttatgaactc tattgcttgt aaataaatcg ctattaaaaa
     4981 taatgttgaa aaaatgttat ttataattat agctccttct ggatgtgaaa atacattatt
     5041 aattatttga tttcctatag ccataataac agaccaaata tacaatctac ttatatattt
     5101 ttttctgtta ctagtgtgat aaaatccttc aacagccatg aatataaaca gtggtgctga
     5161 tattctgcct acccaatgaa accaaattgg tatttgcatt gtttgtggca taaatgctgc
     5221 tatatggtca aatgtcataa atattaatgc taaaatctta atagtaaacc cacttatacc
     5281 ttttttaaaa aaatccatat catatctcct aattttgttt tatctctata ataatttttg
     5341 ttcctattcc aatttcacta cttacataaa ttttaccttt atgaatctct attatttctt
     5401 tagaaatagc cataccaaga cctgaacctt tatgcatctc acctgtatta gtacctctat
     5461 aatatttttt aaaaatatgt ttcaaatcac ttttgcttat tccaattcca ttatctttta
     5521 ttataatttc tatattatct ttttttacaa tttctacact tatcaaagtt ccttcgctat
     5581 tatgaactat tgaattaaat ataagattgg ttatagccct tctgaataag attgaatcta
     5641 catatacttc aattaaatct tcattacttt caaattcaat attcctattt ttatatctat
     5701 tatcatttaa aatatcaatt attatatctc tgactaaact gactaaattt atttttttct
     5761 tgtccagaac tatagtatta ttttttaatc gagtagataa atttaaatca tcaactaaat
     5821 cttttatata cttagactta ttatatataa tttctgtata ctctcgtatt tcatcttctt
     5881 caaactcata atctttgtca ttaattatct ctgcataccc ttgtatagat gctagaggtg
     5941 tctttatatc atgtgttata tttgccagcc attcctctct caactcttca ttttcttttc
     6001 tctcaacttc atttactctt aaagtatcag ctaacatatt tatattttta aaaacttctt
     6061 catatatacc tctgtcctta agatataaac tataatttcc attagccata gtctcaatac
     6121 tccatagtac gcttgaaatt ggttttgtaa gttttctact aaataaatat cctacaccta
     6181 aagctaccac gctatcaatt actagtgtta taatgataac tttatttaaa gtttttttaa
     6241 tgttattttg agaataggtt aaaattattc ttgatatatc tctactaggt atacctacta
     6301 ttatgtttag atgttttttg tttaaatatt tttctagtac aaaattaaca tactttgttt
     6361 ctatatactt gtaattattc accatttgaa agggtgtata tttttttgga gtatcctttg
     6421 gcacatttac tccataaact tcctctccta aatcatttaa aacctgtatc caggaatttg
     6481 atttttctaa tttttcagct ccaatcttag atagtttaaa ttctccatct ttttcatata
     6541 aatctttttc aaaagaacgt gcaaattctt caggattatt tccagaatct acaactttaa
     6601 aaaagctatt agttgaaatc acatacaata tagatataat atttataatt actactgata
     6661 tagctacaaa cacaattgta aatataaaat ttctagtaat cttccacttc atattctaat
     6721 cttcctttac tgctaattta tatccaagac cttttattgt cagaatatat tcaggctttg
     6781 aaggattatc ctctaatttt tccctaattt ttcttatatg aaccattata gtattgtcaa
     6841 atccaacata atcttctccc cacactttat cccatagttt ttctttactt ataatttgat
     6901 tttgattatg tgcaaataaa gataggattt ttagttcctt tgcagacagt cctatagatt
     6961 ttccatcttt tataagttct gccctacttt catttaatat atatgggcca aatgatataa
     7021 ttttttcttc attcttattt agatttttac tttcgttttg aacatcacta aaataactta
     7081 atcttctcaa atgcgcttta actctaaaag ccacttcctt agggctaaaa ggttttgtaa
     7141 cataatcatc tcctccaatc gctagtccaa gtaacttatc taattcttcg gttttagctg
     7201 ataaaaatat tattggagaa ttggaggttt ttcttatttc tttacaaata tcataacctt
     7261 cgccatctgg taacataata tccaatacaa tcaaatcagg attaatactt ttaaataatt
     7321 ctaatccttc ttttttagtt tttgcagtat atatattatt aaggccctct ttttttagga
     7381 cagtttctaa aagcttcaat atttctactt catcatctat tattaaaaca cgattttcca
     7441 taaacaacct cctgtaataa tttacatata tattagcata tattttatct aaaattcatt
     7501 tatcttattt ttaattaaac taaaatataa ataaaattac tattattttt agttaaaacc
     7561 ttttgcttga tgtaaaattt tcatatgtta ttttagttaa cactatcatt aatataattg
     7621 agattactat agaaaaaact atcaaaatac ttggtacatt atacctaacc atttgttggc
     7681 tcatcataat ccaatttgga gtcactgaca taaattttat agctttatct ggtataaaac
     7741 ctacactaaa gtatggaatt gccattatca atccacctag tataaatgga attacagccg
     7801 atttaaatat agatgaaagt gttaagccta tttgtgcaaa tataaaagaa gcaaaaatag
     7861 tagtcaatat aacaattata tacatttgta ttactgtaag atttaaatta ctatttccaa
     7921 agtaccataa gttatgaatc ggttcattta aatttttaaa atcaaaaaaa ctaaaacctc
     7981 taattcctac taacaatata aaagaaatat taataataga taaaattaat ccattagcta
     8041 ctagttttga taatactact gatttttttc cctttttagt tgtatttatt aaacctgtca
     8101 tattagaatc atattcttta atgtaatttc cagaagttat tataaacatt atcaaaccta
     8161 ttagaggtat tgagccttct atcttgtaaa atatatctgc ataagtatct aaaacatcaa
     8221 actttctaat cagaagaaat cccaaaaata ttaaaaacag ccaaattatc caaacactct
     8281 ttgattttaa tatttttatc atttcagctt taactaaata catcatttct actacacctc
     8341 ccttgcatct tccaaatcaa aataaaacat gtatacatct tcgaattttg cttgaactgg
     8401 ttgtatatta tccccacttg gaatatcctc tgaaacaatt ctaagtacta ctgaatcaat
     8461 atcactttga atacttacaa ccttatattt agattgtata tcatcaactt cacattcatt
     8521 tttaacttga acattatata ctttattttc catgtgactt aatatgtctt tgtgtgttcc
     8581 tttcattaga agtttgccat ctttaatcat tattgtttcc tttgcaactg attctatatc
     8641 ggaaattata tgagtagaaa gtattattat cttatctcta gacatttttg ctattaaatt
     8701 cctaaatcta gctctttctt gtgggtcaat acctgctgtt ggttcatcaa agactattat
     8761 cttagggtcg tttagtaaaa cttgtgctat cccaactctc ctcttcatac ctcctgaaaa
     8821 ttttcctata gacttatttc tcacatcgta aagacctact aaatctaata attctcctat
     8881 cttactattt aaatattttc cccttaaacc ttttaaagct ccaatatact gtaaaaattc
     8941 ttttgctgta aaatttttat aaactccaaa ttcttgtggc aaatatccta aaatatttct
     9001 ataattagca tccattttaa atatatcctc accattataa agtatctgac cttcatcagg
     9061 ttttatcaac gttgttattt gtttcattaa tgtagatttt cctgctccat ttggtcctag
     9121 aagaccataa actccatttt ccagagttac tgtaatcttg tcgtttgcta cttttttacc
     9181 atatatcttt gaaagatttt ctatttctaa tttattcatc ttaactcccc ttttatctac
     9241 ttatataatc tgattttatt actaaaaaac ataatgacat agcacctatc aatattgcca
     9301 tcaaaagtcc tattgctata ttgacaatgt ctatattcac tttaaatatc ataatatcac
     9361 ctagataatt tcctattatt ttattaaaac caaatatcaa atcataataa tttacatttg
     9421 atacaactga aagtaatttt ttaggtaata tatggattac cgacattatc ttagttatag
     9481 atataataaa tataaaaaaa cttattgaag atattgaaga tttacttata aaagacccaa
     9541 atatggctat catggcccat ccttcaaaca tcaaaagctc tatgcctata tttaatatta
     9601 catactttgt tatacttaaa ttagttttca atgcaactac attgtctact attctaaaag
     9661 attctaagtc tccattaaca ggtactcctt gttgcataca agttataata aatattccca
     9721 ttaaataaac agaataaatt actaatggaa ctaataaagc aacaaatatt ttagacttta
     9781 atgctttttt cttctctttt gatgatagta tgatatcttt taccgaagta acgacttcat
     9841 ctacgtaaat attggatata attatagata taatgattaa cattcctaca tttataagtg
     9901 aatttgtaac tctatagtta aatactttcc aaaaactaat atccttatat tcattcacac
     9961 ttagattatc aagttttttc tttgacatta attcaatttt tttagaaaat tcacctgtat
    10021 ctgtttgatt tgaaaactgc tttaaaacat taatcttatt ttgaaatttc tcattaccta
    10081 tgtcgatacc acttctcgta tcttctatac atcctttttc tttatctata taataacgtt
    10141 ctgtttctaa aacaggcgta ataaagatac taatcattag tattaacact aatatcccta
    10201 gagatataaa catactctta gatttagcca atttttttat ttcccaattt accaatatat
    10261 ttctcctcct aaatcatttt aattacacat atatattaaa acttaaatct tatataaata
    10321 taatactaac cttaaataaa gcttaaatta ttttataatt tataggtgtg tttattattg
    10381 gagttacatt tattattttt ctgggaaatt atacattttc aattattttt attatttttg
    10441 atatttttat tgattggaaa ttttattgtt gttcaaattg tagttattct ttaaatttaa
    10501 agatggagtt ttttaagaat agaacctact gtccttatta tggaaagatt attaaatagt
    10561 tgcatataac tttatttatc ttgaatctta tttttaaaaa caattataat tttagaatat
    10621 tttctattct caaacaagca tgttgaataa tgaagcatct tctagtatta tatacttgta
    10681 gaataaaact aaattggtaa aactgggaat atttaacctt aaggagttaa gttatagaaa
    10741 ctagtactaa aaaattttat aaattatgtt aattgactaa tagaatgcga ctttttattt
    10801 ttactaaatt ttttaggggg atggataata tgtttattgg agaaatagac aaatgtacac
    10861 atatactaac tgcttacatc agtagttcat atgattattg taatttttta gatacacagt
    10921 tagatgattt tatatcagaa tatggagaaa cggtagttga aacctgctta catcaagtct
    10981 tattattagt aagtagatac aattaaaatt aaatttttct agaaaaacct ttttagattt
    11041 atctactcta ataaatatat aattttttgt actttataag taactataac ttctgctaaa
    11101 gtaaagatgt attgggagta catctttact ttcccattat agaagttaaa agcatacacc
    11161 ataaaatcaa tcatccaata caaattatat atttcctatg tctaacttaa tttacaagaa
    11221 tcattctata tgcatattct gcaatccact aaacatgctc taaaccttta ctattttata
    11281 cattctctat aaatcataca aatcatatat aaaatccccc taatatgttt aaaccaccta
    11341 taaaataaca ccaatattta gaatattcaa tatcaactgc ataactggtc aataaaaacg
    11401 tataactaga caaatttcaa tatttatata aataatacaa gaaaaacaag cattttattt
    11461 atataaattt tttatttttt gtttatatta aaaaactata ttgattaatt tattttattt
    11521 agtaattatg aaaattc
//
LOCUS       DAGQWR010000131        11510 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.131, whole genome shotgun sequence.
ACCESSION   DAGQWR010000131 DAGQWR010000000
VERSION     DAGQWR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11510
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.131"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(94..519)
                     /locus_tag="MIW95_003070"
                     /pseudo
     CDS             complement(94..519)
                     /locus_tag="MIW95_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
     gene            complement(992..1279)
                     /locus_tag="MIW95_003071"
     CDS             complement(992..1279)
                     /locus_tag="MIW95_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152378.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(1299..1574)
                     /locus_tag="MIW95_003072"
     CDS             complement(1299..1574)
                     /locus_tag="MIW95_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152379.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAIGMLKGVDYEPLIVATQVV
                     AGTNFKFICNATSVTNPPHDYLAEIIVFKPLPCDEHNATITSINIIK"
     gene            1762..2581
                     /locus_tag="MIW95_003073"
                     /pseudo
     CDS             1762..2581
                     /locus_tag="MIW95_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(2840..5407)
                     /locus_tag="MIW95_003074"
     CDS             complement(2840..5407)
                     /locus_tag="MIW95_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBY3152380.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTNQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMDGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIALQSEKE
                     LWGLSEWVKQNNSINLYLQNSPAIKIAEDFMSSVMPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(5410..6015)
                     /locus_tag="MIW95_003075"
     CDS             complement(5410..6015)
                     /locus_tag="MIW95_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY3152381.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6389..7849)
                     /locus_tag="MIW95_003076"
     CDS             complement(6389..7849)
                     /locus_tag="MIW95_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBY3152382.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDRAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8180..8758
                     /locus_tag="MIW95_003077"
     CDS             8180..8758
                     /locus_tag="MIW95_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY3152383.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIKRHNLKLKIEKEKAFGVCNS
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9050..10300
                     /locus_tag="MIW95_003078"
     CDS             9050..10300
                     /locus_tag="MIW95_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3152384.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            10461..11030
                     /locus_tag="MIW95_003079"
     CDS             10461..11030
                     /locus_tag="MIW95_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBY3152385.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVDFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAVAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3804 a         1999 c         1589 g         4118 t
ORIGIN      
        1 tctatcctat ctctacatat aggtttccct atatgctgac tattttagct actaactcat
       61 gactaaattc acgagtgtgc gtagctattt tttaatcaat acaattactg cacatataga
      121 tgcagaattt ttaaatactt cacctaatga aactgctgca ctaatatcat gggctactcc
      181 cctattgtcg ctaaaacctg cagtatcaca taattttgca tccattcctt ctattggttt
      241 tctcatattt acaatgtttg ggtataaagt ttgagaaaca gctcctgaac caatctctgc
      301 cacagggtct aagcaatcaa tatatgatat accagtatct tcatcttcta cttccatcat
      361 tacacatcct gcaagatagt taattgcagt acttttgcca catcctgtcc caccaagaat
      421 tagaattaaa ttttgatttt cagccttatt tattgctact tttccttcat ttaataagag
      481 tgttatttct tctaaaagtt gattgttatt ttctggcatt tattttcccc cttggttata
      541 tattttattt aaataaagtt taatccctta agtagattac aaagttttta gactatattt
      601 aaatttgttt tttattttta ataaatttgc attttcactc aataattatt cttctttgtt
      661 tattaaaata actgtgttgt ataatataat ttttaccaac acagttattt tatttaatgc
      721 gcttatttta ttgtatttta tgtaatacag ttgttttttt atattatatg tccactattt
      781 tcctatggtt attacatata gatttttaat tatatttgta ccattaattt aaacattcct
      841 atactactgc ttaagtttta cacctatacc agcttgaata ttttattttt atttgcttaa
      901 atagttcaac tatactggtt taataggttt cctttagttt aaatagttca cctataccta
      961 cttgaatagt tcacttttat ttgcttaaat tctataccaa tactggctta aacattatac
     1021 taccatgatt gtattgtcta tcttcaacta caataaaaac aggttctaaa tctttagtag
     1081 atgttatttt taattctaaa aaaacattat cagttggtaa tatttttaca tcataattat
     1141 ttaaattatt attcgtgata ctattaaata catcaccttg acgttcaaaa aaaacattat
     1201 caactattac aaaataaaca tcttcttttc tactattttc aacctctata gtattttgtt
     1261 tagttacatt tagtctcatc ttccacctcc tattaatatt acttaattat attaatactt
     1321 gtaattgttg cattatgttc atcacaagga agaggtttaa aaacaattat ctctgctaaa
     1381 taatcatgtg gaggatttgt aactgaagta gcattgcaaa taaatttaaa atttgttcct
     1441 gctacaactt gagtagcaac aataagtggt tcataatcaa ccccttttag cattcctata
     1501 gcttcattaa atacttttaa atcttcttct tcgacagccc taaattttga ccatccacca
     1561 acaaaaagac tcatataaaa acctccttta tatatatatt tatttatatg atagtaccaa
     1621 ttttcttaat attcaatata ttacgtctaa ctggtcaata aaaatgcata actggataaa
     1681 aagacttata aaatagctta actttacata taataagatt attctaaaat atgagacata
     1741 tcttgctttg aatatattct atacaacaat tttccaggcc tcattgccaa gtttaaggta
     1801 actgactttg gattccaact tcttgatgct aataaaaaat attttgatat gttcaaagat
     1861 aatgtaaatt ttctattaga tgatataaat ttagaaggtt atcttaacaa tgtgtataaa
     1921 tatcataatg attttaaatc tgggagatgc gtttgttttt ctatttctcc acaaactatt
     1981 aatggagata tttttcacat gcaagttgta gctgaatgta tagattggga agatgataag
     2041 cctatatatc ttttaatata ttcagatgta acgacattgg taaaacaata tgtctcatta
     2101 tcatttttca tccaagaatt ttatcaagaa atatgataaa atagctaaaa aatatgatat
     2161 tccttctcac cttatagaaa ttgagattac agaaacaatt gtatttgaag atgtcaaagt
     2221 atttagtgaa ataataaatg aaattcataa aaatgacttt aaatgctcta ttgatgattt
     2281 tggaagtggc tattcttcaa tcaatatgct taagggtatt tatgttgata cattaaaatt
     2341 agatagagca ttttttcaat cagaagattt agattctcaa cgtgaaagaa atataattgt
     2401 atctataatc gaactagcta aaaagttaaa aatgaccact gtagctgaga gaatagagac
     2461 cagttttcaa aaagatttct tgcaagaaat aaactgtgat atgttacaag gatacgtgtt
     2521 ctctagacct ttgcaaataa aagactttga aaaacttatg tttcaaaact cctccaatta
     2581 acaatataaa attgcttatt ataagtgtat tattgtatca acaaataaag gaattgtctc
     2641 aaaattgatt ttttctaatc aaatttttat tttgagacat tctcattttt ttgcataaga
     2701 aaatacccac caagctacgg tttttcttac cgctgttcgg tgggtattag tagtattatg
     2761 aaatctgaaa actttaagta ttactacttc cgctaacgca atcacaaagg tcgaaagctt
     2821 caaagcgaaa ataggctttt tactcatcaa acgtcaccgt acctgccaac ccgtccaccg
     2881 ttatcatttg tccgctctta atagttcgtg tggcaatgtg tgtagccata acggctggga
     2941 tgccatattc acgtgcgaca atgcttgaat ggctaagagg accaccaatg tccgttacaa
     3001 tcgcacttgc agatgaaaat aatggtgtcc atgctggagt tgtggtaact gcaaccaaaa
     3061 cacttccagg ttgaaagctt tcaaaatctg ctggactatt cagtacacaa gctggagccg
     3121 tcacaacacc agtactggta ccaatgcccg tcaacacaat tttcccatct ttctgcttct
     3181 gtggtgcttg tgatatagtt tttttgtttt tctctggcaa cttggacggt gacacataac
     3241 ccatgtattt tttaagttca gcttttcgaa caggtattat cccgttcata tcagatagag
     3301 acttgttttt atccaactgc tctataagta cctccaattc cgattttgtg agccaataaa
     3361 tatcatctaa atgtgaaata gctcctcctc gtatgaatcg ttctgagatt tcctgaaaca
     3421 tacaacgaat cagaggatgt cccattccca tcaaataaat agcattttca cgcataggag
     3481 aggtttcctg tgcccaatgg agcaacttaa aaaatagttt tttacgcaat ccacctatgt
     3541 gttgtaaaat ctcttcttct gcatgttttc tacgcttttc aaacgtggtc tggcgtaaaa
     3601 aaggactttc cccttttcct tccacaaatg tttttacgga ttcaaaagtt ggtgtaagtg
     3661 tttcctgcgg tgtggaatac gcaaaatcaa actcataagc ggtacgacca aactctttaa
     3721 agtacttatt gattcgattt ttccattcta tccatacttc ctgtgaaact ccaacaggca
     3781 taactgaaga catgaaatct tctgctatct ttattgcagg actgttttgc agataaaggt
     3841 tgatgctgtt attttgcttt acccattctg aaagacccca caactccttt tccgattgca
     3901 gagcaatcgt atcaaaccca agcaggaaaa cggaagtatc cgttatacca gcacgtcgaa
     3961 ctgctccttg aaataatttt gtaaataatg tttcactgat agaagcagca ggcaatgtaa
     4021 gctgaatcct tgtgaaataa atacatgcgg cataaaatat tgtctgaata ccatccataa
     4081 tttgatgggc gttcatcgta tgcaggggct tttcttccca ttgtttaata gcatcctcat
     4141 atccttttcg tgccgtttcc cagcgtgcaa cgctgttcat aagtgtccta cgtaaagagc
     4201 gtggggaaag ggatttgata gcaatcaaaa gtggctttga tttagcagaa agataaacat
     4261 atccattaat aaccgtatat gcgccgtttt ttggtaataa ttttttcgca cttttaccaa
     4321 acatatctat ccataaaagc tccgatgcac ggtttacgat ttcaagacca agcgtggcga
     4381 acaaaggtgt aacaggattt ggcaagtgtt ctgccaaact tccctttgtg tatacaacat
     4441 cctgttctgg caatacccat tccggtggca gaacagtaat tggacgagct tgaacaatgt
     4501 acaatttatc tttttccagt gcccactcta catccatagg catttgataa tacttctcaa
     4561 ttttctttcc aagctgtatc agttgcatta cctgattacg agtaagagca tatttttttc
     4621 tcaacctttc aggaaccatg gtttcttctg ttccttctga agtacgaacc gtcataatct
     4681 ctttatttgc cacttcatac gatataattc gttcggagtc cttatccact acgattgtat
     4741 cgggtgtaac taagctagaa accaccgctt caccaagccc ccatgcagcg ttaataatca
     4801 tttcgctacg tctaccattg attgggttta acgtaaacat aataccagat gaatcggaaa
     4861 atgcaagctt ttgtactaca acagcaagtg cgacaatctc gtgctttata ttattcttca
     4921 cacgataagc aatagcacga gctgtccata aggaagccca gcatctcttt acagcgtcaa
     4981 taactttatc ttcaccttgt atgttaagat aagtttcctg ctgacctgca aaagaagcgt
     5041 caggtaaatc ttcagcagtc gcagacgaac gaacagccac cgatatgttc cctaatccgg
     5101 cataagctgt tttaattgcg tccgacactt cctgtggcat ttctccatta tggaaaagct
     5161 cccctatttt cttagataca tcttcaagtt ggttggtatt attggaatca ataccatcta
     5221 gcagtttatt aattcgagac tgaatatggt tcgtctcaac aaaaatctgg tatgaagcag
     5281 tcgtaacatg aaaaccgtct ggcacaggga tgcctgctgt caatagtttt gataaagaca
     5341 tcccttttcc accaacaatt tcaatggttg cttgtttatg ttccagtggc aagacataat
     5401 aaatcataat cattcacctc catcattcat aattccgtgc agaaatatat tcattgtttc
     5461 atccaacatt ttttccgaac tatccgatgg tcgtgacgtg tagctgattg ataacatgga
     5521 gttaattaac aaacgtgtca caagagcggt atccgttttg cgaaagatgc ctgtggtcac
     5581 accattatca attaccgatt ttataatatc ttgatatgca taacgagcat ctttaagtct
     5641 ctttcgatac tcttcttcta aataatcaga ttctgtattt aaccacatca gtacagttct
     5701 atactgctct ggaaatctaa gatgattaag catgattttt ctaaggcact gttgtggtga
     5761 aggttcacca tcaataatcc tatgaacttg ttggatagtt ttttctattt ctttctcaac
     5821 tgcatataca acaatctcgt ctttcgtttt gaaaaaatcg tatagacttg actttcccat
     5881 gttggctaaa actgcaattt ctctcattga tgttttttgg aatccatttt cagccatgag
     5941 actcaaagca acagcaacta ttttttctcg gcgttctgct tgttgttcag gtgtaagcac
     6001 aattcctttt ggcatagggc gatacctcct tttagcgacc aatggtcgtt tcttttttat
     6061 ttatcataca gcgaccatcg gtcacttgtc aaggttaatt tttttcattg tatatggtac
     6121 tcgagataaa aatgttcatt ttagaaattg ctctatttaa gtagaaatag gcttcatcat
     6181 tagaaaacaa gactataaat ttgagatatt tatcatgttc aaatttcatc aatatcaaag
     6241 tttaagatat taataaaaaa agaattacta agaaataaca tataaagatt caaaaaagga
     6301 gttgaactta aaattcaatt acaaaaaatc aaatttaaga caactccttt attctttatc
     6361 tctactattc tttatctcta tttatttatt acaactccct aacatttacc actactgcct
     6421 taggtctagt catagcctcg attgaatact taacaccttg agttcccata ccagatgcct
     6481 ttacacctaa aaatgggaag tggtctggac ctctttcagt cttattattt atttgaacag
     6541 taccaacttc aagtttatca gctatataaa atgccctatc aatatcactt gtaaatactg
     6601 atgattgaag cccatactct gattgattag caatttcaat tgcttgattt atatctttaa
     6661 ctcttataat tggaagtact ggaccaaatg gttcttccca agcaattttc atatcaacat
     6721 ttacattatc taacaatgtt ggatagaata aattattttt tctaacatta cctgttatta
     6781 actttgctcc tttatgtaat gcatcatcaa ccaacccttg aacaaaatca gttgctttat
     6841 tatctataag aggagtgata actacttcat ccattggatt tccaattgtc aacttctcta
     6901 ccaatggctt tatttttccg actaatttat ccgccacact ctcttgtact aaaattctct
     6961 ttactgccgt acatctttgt cctgaatatg aatacgctcc agatactata ttcttagctg
     7021 caaaatctaa gtctgcatct tctaatacta tagccgcatc ttttccacca agctccaata
     7081 atagtggtac cattccagat attttagata tatgctgacc aacttcagta ctaccagtaa
     7141 agttaataaa attaattcct ttatgcgtta caacataatc ccctatctca ctacccctac
     7201 ctgtaatagt attaagtact ccatctggta tcccagcttt tctcatgatt tctaccacat
     7261 gaagtgcgct aatagcccct tgtgtagcag gttttaatac aactgcattt ccaccaataa
     7321 gcgctggagc aatctttgac attgataaat ttacaggata attaaaagga gatatagcta
     7381 aaacagtacc taatggaacc cttgaaacat aagacatctt atttcttgtt ccaccaggga
     7441 aattatctcc actaatggct tcaccctcca tattttttcc cacatcagct gtgtatctta
     7501 aaaaatctgc tgttctctca acttctgccc ttgcagattt tatgtctttg gctatttcaa
     7561 gtaccagtat attagctatc tcatctatat tttctaacaa taaatcagca gccttatgaa
     7621 atatattggc tctcttgtat acaggcattt cagcccatac ttttatactc tcttttgtgt
     7681 tttttataac ctcatctacc tcatgttttg tcatagattg tactttccca acaagagaat
     7741 tatctattgg tgagtaaatt tctattggct ttctcgaatt agattctacc cattttccat
     7801 ttattaaatt tttatataca ttttcttttg ttttaagttc attaaacata tttttattcc
     7861 tctctctgat ataactttgt gtatttaaat tatagccatt tattcgttat gtaaacattt
     7921 ctttttttca taaacgttta cctataaaaa aaattttaag ttaacgtttt tctagattct
     7981 tctattttaa taacatactc gctccaaatc tatataggtc acactttaat atcaatatac
     8041 caatatacca atataccaat ataaatatta taaacataaa tcacttgaca aatttataag
     8101 tagacaatat tatatatcta tgacttaaaa tatttttaaa gtcatagata gcatatgcag
     8161 atagaaagga ttgataaaaa tggctaaaag ttttacagaa atagaaaaag acaatataag
     8221 aaaaagacta atttcagaat gtgaaatcaa ctggtctaaa tatggataca aaaaaactaa
     8281 cattgatgaa ctttgttcaa aatcaggaat cgcaaagggt tctttctata cattttttga
     8341 ttcaaaagaa aagttatttt ttgaagtact aaatggaatc caaaacagac ttatatcatc
     8401 agtagatgaa attttaagta acatgcctcc aaaagaaggc tttgcaaaat ccttaaaaat
     8461 gttatatcga ttatatgata aaaatccatt tttatattca ccaaataatc ctgatttcat
     8521 cagcctaata aataaactac ctcaagatat gtttttacaa cttgagtcca ataacataca
     8581 aagtttttac gacttaatta agagacacaa tctcaagttg aagatagaaa aagaaaaagc
     8641 ctttggtgtt tgtaattctc tactatcaag tctattggta aaagaaaata ttgaatataa
     8701 ctatattgaa gtatttgatt ttatggtaga taatcttgta gaacacatac ttgaataaca
     8761 atttaaaata atttttctaa atatttcttt atactaaaga ggattaatat gattgaatta
     8821 atgaaaaaaa taaacaatat catttacaaa ataaatgata aactaaatat acacacaaaa
     8881 cttatagacc ccagagttaa ctcattccac tctactctaa gccatctata atagttcgta
     8941 tatttctaat aatattagat attaaagtaa gaaaaaaatt ccttataata attgctatta
     9001 tatcaattat tataaatatt ttaataatct tagataggta ggtatatata tggtaaaatc
     9061 ctcaaacact tttaataaaa acttcaactt gatgatatta ggtcaaataa tctcattgtt
     9121 tggtgcatca atacttaaat ttgctttatc tttatacata cttgatataa ctggtaaagc
     9181 agaaatattt gctactatac tagcagtatc ttcaatccca attatagtat tctcccctat
     9241 tggtggagct attgcggata tattcaacag aagaaactta atggttattt ttgatttttc
     9301 aagcagtctt acagtactaa tcttagcagt attcttattc aacaataatg gttcaatatt
     9361 acttgtagga attattatga ccatattatc agttataagc acaatgtatc aaccaactgt
     9421 tcagtcaagt acaccccttt tagttgataa tgagcattta atgaatgcaa atggtattgt
     9481 agctggagtt gcatctctta caaatattgc aggacctgta ttaggaggtg tcttgtatgg
     9541 tgtgattgga attaatgcaa ttatagttat aagttgtgta agtttctttc tatctgcaat
     9601 tatggagata tttatacaga ttccttttat caaacaagag caaaacagca acattataaa
     9661 aactatattt actgatataa aggatggtat tgttcatata tgtaaagaaa atagatttat
     9721 actaaaaata ctttttttag ctatagcaat aaacttattt ttaagctcta tgttaatagt
     9781 tggtatcccc tatatagtaa aaatcactat gggagctagt aacttcttat atggtctagc
     9841 agaaggagca atttctttta gttcactggt agctgctgtt agtattggta ttttctcaaa
     9901 acatctaaaa atcactaatt tgcacatttc ctttattata tgtgcagtta tgttcatacc
     9961 aatggcggtt tcagtatatc catttatatt aaacacagga tttccagttc catttttaat
    10021 ctttatagta tcatctatgc taatattttt tacaacgact cttatctcaa tatatgttat
    10081 aacagtcaca caattaaaaa ctccaaatga actcttagga aaagtaatgt caattctttt
    10141 tgcaggaaca gcaattgcaa tgcctcttgg tcaggttatg tatggtaaac tttttgattt
    10201 attatcagat agagtgtaca tcattatact aggagttgga tttattacac tacttatagc
    10261 atttgtatca aagataacat tgaggaacga aaatctttag atacaaagaa tttagatata
    10321 aagatttaat tttcactaat ttctgaattt aaatgcaaaa ttgcgtatca gtatgttgac
    10381 gttttagaaa ttgattgata taatcaatgg aaaataaact ttataaaaat tcaaaatatt
    10441 cagctagagg agtgataaaa atgagttaca tgccaacttt agaacaagca tgggaaatat
    10501 taagaaaata caataaggaa gaatttcata taagacatgc tcaaattgta tctggagtta
    10561 tgagatacta tgcaaaggag tacgaccctg agagagtaga cttttgggaa atagttggac
    10621 ttttacatga tttagatttt gaaatgtatc ctgatgaaca ttgtgttaaa caaagagaat
    10681 taatgacaga gcttgattta gataaatcaa taatcgattc tacaataagt catggatatg
    10741 gattaactgg ttcagatgtt aaaccagagc aatttatgga gaaagtatta tttgctgtgg
    10801 atgaacttac tggtttaatt ggtgctgttg caataatgcg tccttcaaag agcgtttctg
    10861 atttagaatt aaaatctgtt aagaagaaat ttaaagataa atcatttgct gctggctgtt
    10921 ctcgagacgt tataagaagt ggtgcagaaa tgcttggatg ggaacttgat aagcttatcc
    10981 aaacaactat agaagctatg agaagcttga ttccagagat ggcaatatag ctcctcatac
    11041 atataccata gaagttacat ctttaaagag catttctctt tttgatgcaa cttcttttta
    11101 tattttgtag aataaaaagt ttaatatttt aaactcacta tataatctat aataaatact
    11161 ataaataata taatgtataa tagtcataaa ctttctaacc ttaaaagttt ctaatactca
    11221 ataaacaatt ctaatataca agtggcatta tatgatttaa ttatatatct tcaatttata
    11281 tattaaaata aatatatata ttaaattctt atacaccaaa tttttacatt aaacttctat
    11341 gcaccacatt tatacatatt aaattcttat atattaaatt tctatgcatc acttttatac
    11401 atattaaatt cctatatatt aaacttttat gcgtcacttt tatacatatt aaattcctat
    11461 atattaaact tttatgcatc acttttatac atattaaatt cttatatatt
//
LOCUS       DAGQWR010000132        11444 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.132, whole genome shotgun sequence.
ACCESSION   DAGQWR010000132 DAGQWR010000000
VERSION     DAGQWR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.132"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            97..795
                     /locus_tag="MIW95_003080"
     CDS             97..795
                     /locus_tag="MIW95_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152386.1"
                     /translation="MKISSQYRSQYSFRHESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            897..1445
                     /locus_tag="MIW95_003081"
     CDS             897..1445
                     /locus_tag="MIW95_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152387.1"
                     /translation="MEGLGFYAAALSGSSYQRIGFGKLDPIEVIADGDWISYKQAQDT
                     LTVIRNFLNSFDWRNASEMERANRAAKLVTEAKYVDSKYCNIVYGNLVDKRGVCGSFA
                     SSFHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRSDGSEISAFGGALDFDYRKLK
                     DATREMTTYYDAKALSILGFNQ"
     gene            complement(1846..2802)
                     /locus_tag="MIW95_003082"
     CDS             complement(1846..2802)
                     /locus_tag="MIW95_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY3152388.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDNNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSYGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIQFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENKSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3505..4500
                     /locus_tag="MIW95_003083"
     CDS             3505..4500
                     /locus_tag="MIW95_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY3152389.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVLKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            4478..5341
                     /locus_tag="MIW95_003084"
     CDS             4478..5341
                     /locus_tag="MIW95_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBY3152390.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            5338..6132
                     /locus_tag="MIW95_003085"
     CDS             5338..6132
                     /locus_tag="MIW95_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBY3152391.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISKLNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            6417..7796
                     /locus_tag="MIW95_003086"
     CDS             6417..7796
                     /locus_tag="MIW95_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY3152392.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKELLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            8251..8964
                     /locus_tag="MIW95_003087"
     CDS             8251..8964
                     /locus_tag="MIW95_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3152393.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSAVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(9236..10774)
                     /locus_tag="MIW95_003088"
     CDS             complement(9236..10774)
                     /locus_tag="MIW95_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBY3152394.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVEEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         4156 a         1299 c         1724 g         4265 t
ORIGIN      
        1 taactaatat ttgtaaatgt atacttttga tgataaaata taattaagtt aagttggttt
       61 ttataaacat aaatttattt tagagaggtg aaataaatga aaatatctag tcaatataga
      121 agtcaatatt catttagaca tgaaagtaat ataaataata caagaataaa tgaaagtatg
      181 gttaagaaaa atgaaactgt aggaaaagac acttatttat ctaatattat gaaacaaaag
      241 caagaactta atgatagaat tagagattta aaatatagac aagaggttta tactaaaaaa
      301 ataaatgaag caattaagaa cttatataaa tcagaaataa gagaaacaac taataacttt
      361 tctaatatag aaataggtat taaaaatagc attatagaag ataaaaataa aagtacaatg
      421 ttagatgaaa attcaactta tctaaataca aatgatgaaa aagaatcttt aatcactaaa
      481 gagtctaatg aaaaaattga agaagaaata ttaaatgatg aaaaattaga agagttagaa
      541 caaaaaaagg attataaaga ggattctaat aaaaaagaga aagtatctga ggacttatct
      601 ttagtaggta aaactcgtga agagcttgaa aatatgctta aaaattttat aaatttaaca
      661 caagaagaaa taatgaaact tgagtcgaga atagaaaagt tagataaaga tgctgaagaa
      721 tacaaacaaa attcaaagac taatatattt gataaaacag atgaacaaaa aaaacatata
      781 aatgtactga tttaaacaat atgttttaaa aaaataagtt taatttactc tatggaggaa
      841 atccaaatgg agttgagctt cgagcagatg gtaaatatta ttatataaat aagggtatgg
      901 aagggctagg attttatgca gcagcattgt caggtagctc atatcagaga ataggctttg
      961 gaaaacttga ccctatagaa gtaatagccg atggagattg gatttcttac aaacaagctc
     1021 aagatacact tacagtaatc aggaattttt taaatagttt tgactggaga aatgcatctg
     1081 agatggaaag agctaataga gctgcaaaat tggtaaccga agcaaaatat gtagactcta
     1141 aatattgtaa catagtctat ggtaatctgg ttgataaacg tggtgtatgt ggaagttttg
     1201 caagtagctt tcatttatta acaaggttaa tgggaatgga ttcactatct atattaaatc
     1261 catcattaaa tcatgcttgg aactatatac aaattgatgg gaaatggtat aggtctgatg
     1321 ggtctgaaat atcagctttt ggtggagcat tagactttga ttaccgtaaa ttaaaggatg
     1381 caacaagaga gatgactact tactatgatg ctaaggcatt gtctatatta ggatttaatc
     1441 aataaaaata ggggaggctt tataaagagc ctttttttat tgatttgaaa tttaataagg
     1501 aattttatat gaaaatttaa tcaataaaag caaaaaaggc tctttataaa gcctttctaa
     1561 gtgttttttt aagtaaaatt ttttatatca acatcagttt taatcaaaaa atataacatt
     1621 ttggacttac ttttaaaaat aaaattatta aaatataata ttaatcatgt taataatgtt
     1681 tttagtttat tatattctat aatagtggtc atgttgacta ttgtagtttt attaagaata
     1741 gttggtttat ataaatatat gcaaagatag cttgttgttt actttactta ttagtatata
     1801 ggacaagata ctctaataaa tttgcctatt ttaatatctt ttgtactaaa accaactaaa
     1861 gtttccttta ttatttcgtt ttgaataata caaagcataa tccgattgtt tgtataaaga
     1921 ctggaaagta tcagatttat tttctaattt agatatacct atagaacaag aattactctc
     1981 tagacataat tctaaaactt tctttgcttt attttcaaca ttctcttttg attgattact
     2041 atattcaaaa acagcaaatt catctccacc aagtcttcca ataatagcat catttacgaa
     2101 tactttagtc aaaatatttg aaaactgtat tagtatttca tctcctcgaa catgaccaaa
     2161 gatatcattt acctgtttaa aattatctaa atcaaagatg taaaagtatc catatgaaac
     2221 agttttaagc atttctttta tgtgtacttc aaaaaaatgt ctattgtata gtccagtcat
     2281 aaggtctttt tctgatttta gtttaaattc atttgccatt gctagtgctt cttgtgcttt
     2341 ttcagttatt gttttagggt aatactcatc tttcatttgg tcaaagtttg gaatagaatg
     2401 gtgaacatgg ataagtttaa tcatatcatc tgtaactctg tataccatag taaatcgagt
     2461 atccatttct acatagatat ctttttctag gtaatctttt tctattaccc aaagagtacc
     2521 atatactata aaagcttcgt ctgtaacttt ggtacatgaa taccactcat ctataatctc
     2581 aaagtctatt tgctctgatt ctgtttggtc tttttcaaga cctataataa attcttctaa
     2641 tgtttcgtaa aattcatgtt ttcctgttcc aattaatgca aggttgttat ctaataagga
     2701 aataataaat ttaaggtttt cttcttctgg atttaaaata taaatatgcc aaattttttt
     2761 tgataaatct cttaattgtt tttgtattgt attattttcc atagattacc cccttaagat
     2821 ttacagttta tattttactg tattataagt tgaactctta ttacaatatt attataatat
     2881 agaactaaag aaattacatc ctgtataggt aaaagtatat cttttacaat aaaaatatta
     2941 cagtaaaata accagtttta atatacgtat tatattttta atttataaac gaatattata
     3001 aatgaaattt aaattcaacc ctgaagaaat aagctaaatg tattagtata taaatccata
     3061 cttgaacata aataaccttt atgatatgat atcaataata atattatgag aggagtgcct
     3121 tgatgtcgga gttatataat ggtaaccaac aattaaatag ttggagacac atatattctg
     3181 tataatcaga aattttaagt gtatttttaa tacatttaat ttttgtgtct tattctgcct
     3241 ttatagataa ctgacatatg acaacaaata gtatttaaaa tatatattac tttgttaaat
     3301 tatggtgtag taggctttaa tctgctacac ttttattaat tttgtatagt attattttga
     3361 taactatttt gaaatagaga gcattaattt ttctagtcca caggagtttt ctgtttttgt
     3421 gctgttaaat ataagcacag aaaaaggcag atatatacct gtggactttt tttatataaa
     3481 ttatgaaata tggaggtagt taaaatgagt ataattgaac ttaatgaact gactaaatct
     3541 tatagaagat ttgagaaaaa ggcaggattt atggggagtg taaagtcttt atttaatagg
     3601 aaatatacag aaaaaacagc tatatcgtca tttgatttaa agatagaaga aggagaattt
     3661 gttggattaa taggtcaaaa tggagctggc aaaactactc ttattaagat gttaactggt
     3721 attattgcac cgtcttctgg agaaatatct gttatgggtt attaccctaa caaattagaa
     3781 aataatttta aaaaacaata tgctgttgtt atgggccaaa aaagtcaact attctttgaa
     3841 ttgacaccag cagatacgtt tcttttattt aaagaattat atgatatatc taatgaagaa
     3901 tataaaaaaa acttggatta ctttatcgaa ttatttgagg tatctgacta tttaaatgtt
     3961 caagttagga cgctttcttt aggagaaaga atgaaaatgg aattaatagt agcccttcta
     4021 cataatccaa aaatattatt tttggatgaa ccaactatag gcttagatgc tatagcccaa
     4081 aaacaaatta ggtatttcct aaaagaagta aatgaaacaa aaggaactac tattatactt
     4141 acttctcatt acatggaaga tattaagagt ctctgtagta gaagtattgt tgtagaaaat
     4201 agccgtaaaa tatatgatgg ttctacagag aaactattta atcattttca aaaatataaa
     4261 aaaataacag ttagtttttc caatgaaact aacattgtat ttccaaaaga ttgtaaagtt
     4321 gttgaacaaa caaatcataa gacatcagta attgtgctaa aagaaagagg aaatgaaacg
     4381 attaaatgga tttttgataa atatgatgtt aaagatgtaa tgattgaaga agaagatata
     4441 ggtagtattg tagagagaat atataagaaa ggaagtgatg agtattatga gcaactataa
     4501 tgaagcaagt tcaaattatt caatgtttta taaatttaaa caaagtatta gaaggtatta
     4561 tgaagtagcc aaaattaact ttaaagtgca acttatctgg agatttgatg tggtggttaa
     4621 tatggtgttt acgattgtaa aaattttatt tgcttatatt ttatggaaag caatttttgg
     4681 tgaaaataaa atagtctctg gttttacatt taactctatg ttgtcttact atataataag
     4741 ctcattttta gcaggtattg atatgtcaaa gagtaccagt gaagagatat gtaatcgtgt
     4801 aaaattgggc acattttcaa agtacatgat tattccaatt aatgttcaaa attattttat
     4861 tgctaaaaat tttggaatga catgttttta ttttgtattt aatatgattt ctgcatttgt
     4921 atggattttt atatttaaaa tagattttgc ctttacatca aatccagttc aaatattaat
     4981 ttcaattttg atgatttttc tagggtttat ttttatgatg aaactccact tctttattgg
     5041 gattttgtca tttaaatttc ttgagattag tttttttaga atgataattg aaaatattgt
     5101 tgaatttatt acaggaacaa tgataccact tgtactctta ccagaaacta tagtaacagt
     5161 tatgaaggta tttccatttt attatacaac atatttgcca tctatgttac taataggtag
     5221 aaacagcaat gaggcaatac aaggattaat tattctagca ttatgggttt tatttttttc
     5281 tgtagtaaat aaagttatgt ataaaagaat gagaacatta tttgatgggg tgggtatatg
     5341 atgaaaaaaa atttgaaaat aatatttatg ttattaaaaa tgaaattatc aaagatgatg
     5401 gtatttagat ttgatttttt tggagcattt tttgtagata gttcattatt tatattacaa
     5461 cttctaatgt ttaatagtat atattctcat gtagactcaa tagggggatg gcaacaagga
     5521 gagatgttaa tatttatagg aactttctca ttaataaatg cgataaatat gacgattttc
     5581 ttttttggta tttatgatat tccacgaaaa atccaagaag gagaattaga ctactatatt
     5641 acaaaaccat tagacccact atttaggtta acatttgaaa atattaatat tggttcatca
     5701 ccattaattc tagcaagtat acttattgtt ttatatggaa tatctaagtt aaactcagag
     5761 ataactttag ttatgataat aatatatatt ttcatggtgt tattgatgac attacttttt
     5821 tatgatgtat gtatcatact tagaacagtt tcattttttg ttatatcatc ttctgcgata
     5881 atgagattag aagataattt attgccaatg aatatgaaaa ttcctggtgt aatatatgaa
     5941 ggtgttttta aagtattatt ctatttaatc ttaccatatg gaattatgtc gacaattccg
     6001 actcaaattt tggctgggac aataacaatt aatggattaa tatactcaat tttaattgta
     6061 tttttgttta cattgtttat gtggagattt tggaagttag gtatgagaca ttataaaagt
     6121 gcaagtagtt aataaattag taaaaataga caatgtataa ctatatataa atacgtttca
     6181 tatgacacta aactatgtga acaagtattt gcgctaaagg cactgaaagc tataaaatga
     6241 ttaaagtaag gatttaaaag tttctttagt agaaatataa ttgtgtaaaa aagcaagatt
     6301 aattaaaact atatccaaat aattagatat aattcttatg cagaaaggag gtctgtaaat
     6361 gtctaattgt ttggaatata ttttaaaaat aattaatgta cagaaaaata aacaaaatga
     6421 gtgatgtata ttattcagaa aatcatgaac aagataatct ttatgagcaa gataatttac
     6481 tggcacaaag atttaattta tttactctat tgaagtttgc atttcctact atttttatga
     6541 tgatttttat ggggttgtat acaatcgtag atactatatt tgtatcaaga tttattaata
     6601 caaatgctct atcagcaatt aatattgtat gtccaattat taatcttata gttggaattg
     6661 gaactatgct tgccactgga ggaagtgcta ttgtagcacg taagatggga aatggtgata
     6721 ttgagggagc aaataaagct ttttctttaa tcgtattatc aggagttgta attggggtaa
     6781 taattactgg tataggtatt gtatttatgg attatatcat tatatggtta ggagcaagtg
     6841 aagttttaat taaatattgt cgttcatatc tttttattat cttggttttt gcttctgcaa
     6901 atatactaca agtattattt caatgtttat ttgtaacagc tggaaagcct caatttggat
     6961 tatggctagt gttaggtgct gggtgtgcta atataattct tgactatgtg ttcattgttc
     7021 tgttacaaat gggtattagt ggagcagcac ttgcaactgg agtaggatac ctaattccta
     7081 ccgtttcagg tatggttttc ttttcaaaaa ataaagagtt acttaagttt tgtataccca
     7141 gactagataa aagaatatta tttgaagctt gttttaatgg ttcatctgaa atggtaggtc
     7201 aactatcaac agcagttact acttttttat ttaatgctat gatgatgaaa ttaattggtg
     7261 aggatggagt tgcagctatt acaataatta tttattctca atttctttta acttcgcttt
     7321 atataggttt ctcaatgggt gttgcaccaa taataagtta taactatgga agtgaaaata
     7381 atacacagtt aaatcatgtt tttaagattt gtatattatt tatttcagtg gcatctttat
     7441 tggtatttat cttttctata tcatgttcac caatcattgt agagattttt tctcaaagag
     7501 gaagccgagt ttatgaaatt gcaaggtctg gattttatat attttctttt agttttattt
     7561 tttgtgggta taatattttt acttcgtcta tgtttacagc attgtcaaat ggcaaggtct
     7621 ctgcaatatt atcttttcta cgtacatttg gatttataac tataggttta ttgatactgc
     7681 caaatatatt aaatataaat gggatatggt tagcagtacc aagtgctgag attttaactt
     7741 tatttttaac cattttttgt atatgtaagt ataagaatag atataaatat atgtagatat
     7801 taaataaata gtttaggtag atattttatg ttttaataag taattcaatt agtggtagat
     7861 atatactatt gaaaattaaa gttataattg atataaagaa ttaataaaaa taacatataa
     7921 aagtttaaaa gggagttgaa cttaaagttt gattagaaaa agtcaatttt aagacaactc
     7981 cttttatttt actattgtat taatctgtat ctgtatctgt aagtgtatta gtctaatatt
     8041 ataaactttg gatgaaatta attttctata ggttttaatg tatcatacac tttattttaa
     8101 agaaacttat cttaattttc atagtgattg aatttgtcta ttagagatgt tactattagt
     8161 aatgtaaaag caagagtagt gaatataaaa aaacaagata ttgatactgc aagattagaa
     8221 tataataaat ttttatggag agatttcaat atggatacat attttaataa aaaaatatta
     8281 cttgttgatg atgaaaaaga tatagtagat ttaattgaag aagtactcat aaatgatggt
     8341 tttaaaaaca ttattaaagc ttataatggt ttagatgcta tttcgctgtg taagatagca
     8401 tgtccagatg ttgtcattct tgatataatg ttaccagata tagatggtat agaagtgtgt
     8461 aaaaaaattc gagagttttc atactgttct attctttttt tatcatcaaa aaatgatgat
     8521 attgacaaaa tactaggtct tagtagtggg ggagatgact atataacaaa gccattcagt
     8581 ccaagagaaa ttgcttttcg agttaaagcg caattacgta gacaacagta tcaaagcatt
     8641 gtaccatcag atagtgcagt tattaaaata ggagatatta ctattgatat agaaggaaac
     8701 cgtgtatata aagatagaaa tgagattgag ttaactggaa gggagtatca tcttttaagc
     8761 tacatggctc aaaatgtaaa taaaattata ggaaaagaaa gactctatga acaagtatgg
     8821 ggagtatata gtagtatttg tgacaataca attatggtgc atatacgaca tataagagaa
     8881 aaaattgaag ataacccctc aaatcctaaa atacttataa cggttaaagg actaggatat
     8941 aaacttgtga atagaatcga ttagaataga ttttaatatt aatgaagatg tctcaagttg
     9001 aatttttagt taatgaggca ttcttttatg tgtgaagtca aatgtatttt cgttatctca
     9061 acaatgaaga agaggtctat ctctattttg agatagcctc attaagttta tatcctggta
     9121 atgtacttta tgttctaata aaattttttt agtttatttg cagtgaaaca ttaactagaa
     9181 cattaaaatt tgcattgaaa tatcaattaa ataatattgt atattgtgta taaacttata
     9241 ctaattgttt ttctatatta aacactttat caatccaacc tttataaaat ttttcttcat
     9301 gggaaactag gattatactt cctctaaact gtttcaaagc attttgtaaa gcatcctttg
     9361 tctctgcatc aaagtgattg gttggttcat ccagaataag aaaattacaa ggagataaca
     9421 tcatacaaca taatttaacc ttagattgtt caccaccact taaagtagat acactccttg
     9481 aaacatgctc ttccttcaca ccacatctgg ctagatgacg tctaatctct tttgtattta
     9541 attttggata tttatctgct actatttgaa gtggggtttt atcaggattt tcccatttta
     9601 aatcttgctc ataataacca attttaactt gttctgaaaa ctggaaatct ccagatatac
     9661 gaggaatatc tttgactaat gtctttaaca aggtagattt accgattcca ttaaaaccag
     9721 ttataactat tttttgacct ccatcaacag aaaaatttaa ttttggaagt aaagaatagt
     9781 agtatccaac ttcaagattt gttatagtaa gagcattttg agccgatatt tcaatttctg
     9841 aaaattgaat atttggtttt cctgtaaaac taggaggagc tattctttct attttatcca
     9901 attgtttttg tcttcctctt gcaatttttg aatttactcc agctttattt ttacgtataa
     9961 atgtctcttc tttttctatt ttcttttgtt gtgcttgata ccttctaata taatcttctc
    10021 ttaaatgtga tttttgttta aggaactctg aatattttcc atgatatttt ttaatcatac
    10081 caaattctat atcaagaata cctgtagaaa ttttttctaa aaaatcaaaa tcgtgggaaa
    10141 ctacaataaa tgccccatta aatgtattta aataatttga gagccattca acatgttctt
    10201 tatctaaaaa gtttgttggt tcatctaata atagaacatc atggttagaa agtagcaatt
    10261 ttgctaaaat tacctttgcc ctttgcccac cactaagctc ttcaacaaca cgattcattc
    10321 ctattgaatc taatccaaga ccatttgcta ttttatttat ttcattatct atagaataga
    10381 aattacttgc tattagttgt tcctgataat ctgatgcttt gattagatat tgctcatttt
    10441 cagatatagc acttttttga tagagtaaat tcatttcctt ttctatttta taaagttctt
    10501 caaatgcagt gtgtaaatag agtgatattg ttgtatctct attgatttct gcatattggt
    10561 ctaaatggcc tatattgata ttaggttgcc acttgatttc tcctgaatct ggaacaactt
    10621 ctcccaataa aattttaatt aatgtacttt tacctgtacc attttggcca actacaccta
    10681 tatgttcacc tttgaataaa tcaaagttag ctttctcata cagtgattta tccataaaac
    10741 attgtgataa attagttact tttaataaac tcataattca aactcctttt cttttattaa
    10801 aaaatttatg gaaataaaaa aagcagagga atggcattgt aaaatgccga cctctgcttt
    10861 tggaaaaaca aaaggtcata gacaagcatt gttgtctatg accagaaaat ctatactatc
    10921 tatcggcaaa taggtaattt taaataagca tagccaaaaa actatactaa acctatattt
    10981 gccgatgaag atagaaagct ttaagctttc agattaacct ctacacaacg cttcatcgta
    11041 tacatttgtg tagagttaag ttttctaaca agtttaacta attctatata aatcataatt
    11101 attcacatcc taattttagt attaattcta gtttatctat atttaaattg tacttttgta
    11161 ctttgtggat tatcttattt atttaaaata tgttttatta attatagctt aagttatgtt
    11221 atcataatac ttggttatag tcaataagaa agttatattt gctatatatt atattaattt
    11281 agtaaatcta tcattacaaa gttatttgaa gactcgctac caataaaatt ttatagtcta
    11341 tgggaaaatt aagattatga gttttatact tttgatatag ggatactatt ttcataaata
    11401 ttacaacatg tagaagtata tgtctagtat tgttgaattt tcaa
//
LOCUS       DAGQWR010000133        11373 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.133, whole genome shotgun sequence.
ACCESSION   DAGQWR010000133 DAGQWR010000000
VERSION     DAGQWR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.133"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            93..977
                     /locus_tag="MIW95_003089"
     CDS             93..977
                     /locus_tag="MIW95_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBY3152395.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            1076..2251
                     /locus_tag="MIW95_003090"
     CDS             1076..2251
                     /locus_tag="MIW95_003090"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBY3152396.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEELLKDIPTANKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            2379..3233
                     /locus_tag="MIW95_003091"
     CDS             2379..3233
                     /locus_tag="MIW95_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152397.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSNESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKIKDDSSC
                     LYLDIYND"
     gene            3277..4512
                     /locus_tag="MIW95_003092"
     CDS             3277..4512
                     /locus_tag="MIW95_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBY3152398.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREIVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKSISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNEKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            4613..5554
                     /locus_tag="MIW95_003093"
     CDS             4613..5554
                     /locus_tag="MIW95_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBY3152399.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(5783..6136)
                     /locus_tag="MIW95_003094"
     CDS             complement(5783..6136)
                     /locus_tag="MIW95_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBY3152400.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMIW
                     LEKLKEDNTYEIYSI"
     gene            6432..7343
                     /locus_tag="MIW95_003095"
     CDS             6432..7343
                     /locus_tag="MIW95_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBY3152401.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            7603..8037
                     /locus_tag="MIW95_003096"
     CDS             7603..8037
                     /locus_tag="MIW95_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152402.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            8060..9595
                     /locus_tag="MIW95_003097"
     CDS             8060..9595
                     /locus_tag="MIW95_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152403.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVIYK
                     IDCDVILVNLFELIINNCIFSTILGNEVINLEISKYEYEFLEKQFKNMYNNITEDKVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKTEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            9679..11046
                     /locus_tag="MIW95_003098"
     CDS             9679..11046
                     /locus_tag="MIW95_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY3152404.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKARDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMNTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            11081..11305
                     /locus_tag="MIW95_003099"
     CDS             11081..11305
                     /locus_tag="MIW95_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBY3152405.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
BASE COUNT         4523 a         1049 c         2101 g         3700 t
ORIGIN      
        1 atgtattgac atataaatat tatgaattac aatttatata aactaataaa aattaaataa
       61 tattaatcaa cttattgaat ggaggaataa aaatgaaatt tgtgactttt tgtagtagca
      121 atgaagaaaa aataggtgta tttaattcag aaacaaatag tatatatgaa attaatagtt
      181 taggattaag taaattatat actgatatga atgattttat tgaaaacgta tctacaggag
      241 atttagaaaa aataaaaaac aattcatttg aaaacgcaaa gtgttataag ttagaagaag
      301 ttaaattatg ttccccaatt gtaagaccta aaaaagatat tatatgttta ggtctaaact
      361 ataaagacca tgtcaatgaa atcccagatg gagttataaa aaacgtagtt atgccagatt
      421 atccaattta tttttctaag agagcagaca aaataattgg agtggatgat aagataagct
      481 tacatggaga tttggtagag aagttggact atgaatcaga acttgcagta atcataggta
      541 aagaaggtat aaatatatca aaagaagatg cttatgaata tatatttgga tatacaatag
      601 taaatgatat aagtgaaaga gcacttcaag ataaacatgt tcaatggttt agaggaaaaa
      661 gcttagacac acatacatct atgggacctt gtatagtaca taaagaagaa tttgaacatc
      721 ctttaaaatt agatataagt agtgtagtaa atggagaagt aagacaaaat tctaatacag
      781 agtactttat atttgatata cctactataa taaatgattt atcaagagga atgactctaa
      841 agccaggaga tataatttca actggaactc cagcaggggt tgctatgggg atgaatcctc
      901 aagtatattt aaagcatggc gatatagttg aatgtaaagt cgaaggaata ggagttttaa
      961 aaaatatagt tgattaatgg ataaatatgc catataattt tattagtaca aataatttaa
     1021 ttaatatagg attgatcaaa cttatgatta atttattata gagagggatg agtatatggc
     1081 aagagaacta gaattaaagt atggctgtaa tccaaatcaa aaaccatcaa agatttatat
     1141 gaagaatgga gaacttccta tagaagtatt aaatggaaaa cctggatata taaatttttt
     1201 agatgcattt aatagttggc aacttgtgaa ggaattaaaa gaagctacag gtcttccagc
     1261 agcaacttca tttaaacatg taagtccagc aggagcagca gtaggggtac ctttatcaga
     1321 tactttaaag caaatatatt ttgttgatga tttagagctt tctccattag cttgtgctta
     1381 tgcaatggca agaggagcag atagaatgtc ttcttatggt gattttatag cactttcaga
     1441 tgtatgtgat aaggagactg caactattat agcaagagag gtttctgatg gtataatagc
     1501 tccaggatat acagatgaag cccttgaaat attaaaagga aaacgtaaag gcaactataa
     1561 tattgtaaaa atagatgaaa attatactcc tgagcctatt gaaacaaagg atgtttatgg
     1621 aattactttt gaacaaggta gaaatgaaat attaataaat gaagaactat taaaagatat
     1681 tccaactgcg aataaaattt ttacagatag tgcaaaacgt gatttaatta tagctcttat
     1741 tacattaaaa tatacacaat caaattctgt ttgttatgct aaagatggac aagtaatagg
     1801 ggtaggtgca ggtcaacaat cacgtattca ttgtactaga cttgcaggta acaaagctga
     1861 tacttggtat cttcgtcaac atcctaaagt attaaactta aaatttaaaa aggatatagg
     1921 tcgtccagat agagataaca ctattgatgt atatctttct gatgattata tggatgtgct
     1981 tgctgatggc atatggcaaa acttttttga agaaaaacca gagcctctaa ctggagaagg
     2041 aaaaagagca tggcttaaaa ctttaacagg agtagcactt ggttcagatg catttttccc
     2101 atttggagat aatatagaac gtgcaaaaag aagtggggta agttttatag cacaaccagg
     2161 tggttctatt cgtgatgata atgttatatt aacttgtaat aagtataaca tagtcatggc
     2221 atttactaaa aatagattat tccatcatta aaatactgaa agtataaaga taatatagat
     2281 taagtaaaaa tctacaacaa gttttgttgt agattttttt atttgctgta taattttaaa
     2341 tagaacttta tttttagata ttcaatttgg aggttgatat ggctttaaat ataagaaact
     2401 atagcctatt atgtaatcaa ggaacagcta aatttaatga agatgtagtc ggtataaatc
     2461 catttggggc atgggtttta gatggtgcta caggacttaa tggaaaaaat ttagtgtcaa
     2521 atgaaagtga cgcaaggtgg tatgttcagt ggtggaatag gtatttatat aaaaatataa
     2581 ataaagaaga atcattaaag agtattatta atactggaat aatagaagta aaaaaagagt
     2641 atgaactaag attaaatggg tttaaggttg aaaaattaga tttaccatct agttctatag
     2701 ttgttgtaaa atttcatgaa aataaagtag agtatttttt attaggagat tgtgctttgt
     2761 ttattaaaaa tggagattcc aaagttataa aagataggag tatttgtaaa tttgataata
     2821 ctgtatttga tgaaatgagt aaactttcta acttggaaga gatggcattt gatgagatta
     2881 gaactagtgt tatggataca atagtaagca atagattgaa gaaaaatacg aaagagggct
     2941 attggatatt ggattttgat gaggaagctg tggaaaattc tatatatgga tacattgaaa
     3001 taaaaaaaga ttttcaattg atgctaacga gtgatggttt tacaagtgca tgtgatagat
     3061 ataatctaat aaaagaagaa gaattgatac gtatagcaga aaaatttggt ataggatata
     3121 tacataataa agtaagggat tttgaagata atgactataa agcggttaaa gtgccaagat
     3181 ttaaaataaa agatgattca tcttgtttat atttagatat atataatgat tagactgtat
     3241 aaaactattt aattttagaa aggaggcaag tgaaaagtga aaattcttca tataataaca
     3301 caaaaaccaa atagtacagg tagtggtata tatttgtctg gtatgataaa agggttcgaa
     3361 aaaatcggtc ataagcaagc tgtaattgca ggtatagatg taaatgatga tgtaaattgt
     3421 tttccaagtg aagtaagttt ttatccagta aagtataatt gtggggagtt aaattttcct
     3481 gttgtaggta tgagtgattc tatgccatat gaaagtacaa gatataaaga tttaaatata
     3541 gacatgataa atagattgaa atatcaattt aaagtaaata tagataaggc tatgaatgat
     3601 tttaaaccag aacttataat atgtcatcat ttgtacctct taactgcatt tgttagggaa
     3661 atagtaaaag atataaaggt tatgtctatt tgtcatggaa cttgtttaag acagttaaat
     3721 accattgatt tagagaaaga atacataata gcaaatataa ggaaattaga tttaatattt
     3781 gctcttcatg aaaatcagaa atatgatatt ataaagactt ttggagtaag tgaaagtaaa
     3841 gtagttgtaa ttggtagtgg ttataatgat gatatatttt ataataaaaa ttataaaata
     3901 aaggatgata aaataaaaat agtatttgca ggtaagatat gtaaatcaaa gggattgatt
     3961 ccatttataa aatctatatc taaattaaaa tatagtaaag atttaataga agtcaatttt
     4021 gcaggtactg gaagcgatat cgaatcatat aatgagatag ttaaactggc aagtaagtct
     4081 ccatttaaaa tgaatttcct aggaaagtta gaacagagag atttagctga gctattcaac
     4141 aggtcacaaa tatttgtgtt accatcattt tatgagggat taccagttgt agttttagaa
     4201 gctttatctt gtggcacaga tgtaatcact actgatattc ttggtgttaa agagtggatt
     4261 ggtagtgaga ttaataattc tggaaagata gaatatattt ctctaccttt tatggagaaa
     4321 gaaggtattc caaaagatga agagttatat gattttgaaa ataatttgta taatgcaata
     4381 gattctaaga tacaatcttt attgaataac tcaaatgaga aaacatctgt agatatgtca
     4441 aaaaaaacgt gggatggctt agcatataga ataaatgaag ttattttaat ggatgaattg
     4501 tgtttagtct aaaattattt aaattataaa aaagtgtaac tatttttaaa ctttttttaa
     4561 atgattttat attatactaa tactaaatat atttgtttta gggaggtaat ttatgaggat
     4621 tagtaaaaag gtattggcgt taggtatttc agcattactg ttaagtgtta gttttccaac
     4681 atcaataaac gcactagata agattgaaaa tattcaagga gtagataaat atgaaacagc
     4741 tgggcttatt tcagataagc aagattttac aacagcagtt cttatcaatg cagattctac
     4801 aatggtagat gggattgctg caagtggtct tgcaggagta aataatgcag ctattttatt
     4861 aactaataag gatgatatac cagaagcaac acttcaaaga ctgaatagag ttactaagat
     4921 atatgtaata ggtggagaaa attctataag taaagatgta gaaaaaatgt tacttatgag
     4981 aagaatgcaa gttataagaa ttgatggtgt agatagagta gatacaagtt ataaaattgc
     5041 aggtgaaata gaaaaaatta aaaacagtga taagatgttt ttagtaaatg gatttaagga
     5101 tgaagctgat gcagtaagtg ttgcatctgt tgcttataga gatggagctc ctataatatt
     5161 gacaaaagat ataccttcag cagaagaaga cacagactta gacccatggt ttggagtttc
     5221 tcctgttcca gtatatgcta ttggaggaga gtctacatta agtgattaca tagtgagcag
     5281 atatagagca actagaattg gcggtgttga tagatatcaa actaataaaa atgttattga
     5341 aaagttctat aatggagcaa aagagttcta tataacaagt ggagacgatt tagtttatgc
     5401 tttagttgca tcaccacttg caaaaaatgc tcctgttgtt ttagtttcaa ataaaagtga
     5461 taagagtata ttaagtggag ctagtaaagt aacagcaata ggtataagtg ataagagtat
     5521 tatagagcaa tgtttggatg ctgtaaagaa gtaatggagt gttattagta aaatgaatat
     5581 atacattgaa aattagtaga ttgttcattt tataatggta taaaatatat gggaagaggc
     5641 tatcaaatgg agataagcct cttttttatt gataaaatta tgaaaatata ctttatttta
     5701 tataaaaaaa gtatctcatg aactaaatgt ctattttgat atactatctc cttttcttat
     5761 attatatgta ttatatgatt ttttatatgg aataaatttc ataagtatta tcttccttta
     5821 atttttctaa ccaaatcatc tctaaaatat ctagttcttc taaatagtct ttctctgttt
     5881 tgttatcatc atgttttata agttctaaaa cttcaaatgt aaatgctttt tcaccatatt
     5941 ttttccattc attatttaat tcttttatca tacatgagcc agcaccaagc tgaaatttga
     6001 gagagttaaa tttgcttttt atgtcattac tttttccaag gtaaatcttt ttagtaatat
     6061 cattttttat tatgaataca cctgtgtcaa atttcatttc tttgtataat tgttttaatt
     6121 cacgttttct atccatgatt ttagatacct ctctttataa ttgatatgta atatgtaagt
     6181 ttaaaaatat attacatatc aattataaag agattaattt tatatgtaaa tagtttttat
     6241 aaatttaatt atgaattagt catcaataaa caagaaattt agctgtagaa atccattata
     6301 aaggctatta taaattttaa tatgctaaaa atataaaaag aatagttgac agtatagaaa
     6361 atatggaata aactaaaagt gtaatagtaa tacgaaatta tatattacat tgaaaggtgt
     6421 gaatgattat tatgaataaa aaaatagaat taatagggaa ttttgcaagt gattggatta
     6481 ggtatagtga ttatgaatat aaaattactg aagaagatga attatatata gttccaacag
     6541 aagatgctgt gtttaccatg tataacccat ttgatgtagc agatgatata atagtagata
     6601 taattcgcat aggacaagag gcattacaag atatatcaaa agagacaaaa gaaatgaaac
     6661 taaagaaaga agtacttgaa tttgtaaaaa agtatggatt attgggttta atatattcaa
     6721 gtgtatataa tagagatata gttggagaag aaagagtact gatgattgaa aagaattata
     6781 ttacaaaaga gaatatattg agtacagatg aatatgtaaa catgtttata ccatttgcaa
     6841 gtgatgaaga tgtgtatttt aaaaaatata gaaggggtat agatgtatca aagagagaag
     6901 aatctccaaa gttttttgga aagagaccat tgattttaga tttagtattt tcaaagttct
     6961 acactgaaca actagattgg attgtaagtt ttgctaaaga tatgtgtaag cattttaatc
     7021 aaatttgtgt atataaaaat acatctaatc tattaactga aagtgttact attatgtctg
     7081 gagggtttaa tcctcaaaag ataggatttt ctataagcca attagataaa ccaattattg
     7141 catgggagtt tgattctttg aaaacagcta ttgagactat ctatgccttt gcaattacaa
     7201 gtgaaccttc ttctttaaat aaatgcaagt actgtggtaa aatttatatt cctacaaatt
     7261 ctaaatctca atactgttca ccttcttgta gaaattgctc aaatgtaaag aaaagtagaa
     7321 gtagagccaa aaaagataaa taaaattaat aaatatatca ttgtttagat tttagtaaat
     7381 aactactgaa ttaaaagttg ttatagaaaa ttatatatct aaatgatggt atttttttgt
     7441 tgagaaaatt gaaaagttta attgagatag taattttgag ttgacaaaaa ccacttttaa
     7501 cactactatt ataagtggtt ggtaaaataa tatacactaa tttaaatata gcataaaaaa
     7561 caaaaaaagt caatgaaaaa aataaaaaag gagaatttaa aaatgatatc acctattatt
     7621 ttgagttcta taaatcagaa tttaaaggaa atagaaagaa atgaactgct tgaaactaat
     7681 atagaatctg gagattatgg tctagctctt tctgaatctg atgtaaaaga tataataaat
     7741 tcgagagata atactttaaa ggggtatgga agaatagaat tagatataaa agttacaaaa
     7801 cagttaatag aaaatatata tacatcacaa tatacaaatg tggacaatta tttagaggct
     7861 attaatgata tgcaagaaat tttttattat ttaaaaaatg aaactgatga caagatatgt
     7921 gatgatgaga ttatagaaat actgggtgaa ttttatgaaa aattttcagg caatatggac
     7981 aatgtaagag gagaagcaga cgaatttgca aagaaattta aatttggaga agtttaatat
     8041 atgataggag agattttgta tgttaaataa tttaagtaat attaatataa acgaaaataa
     8101 tttaataaag aaccaatatt ccatatctct acttaaggaa tgtctaaatt gtaaagtgat
     8161 agatgaaaga gaagtatata atatacaaca agaaatatct ctaatactta tggatttgat
     8221 taaaaaatat acaaatggtc aaagcacatc agtaaaaaca gaagttgccg aaaaactgtt
     8281 gatttcaata tggtatgcta ttgatgcata tataaataag cttggagata tagaaaaaag
     8341 agttgtggag ttgactgcta agaatgttaa aaaagtgtac cttggaggca ttgaaatatt
     8401 agaggaagag ttagttaggc tgaaagattt ttataaacta atgatgagta gaaaattgga
     8461 tactaattca atagcatata atgatacctt gatggagtta tcaagtttct ttaaaagtta
     8521 tattgtaaaa tttgaagctc aggacgttcc agtaagtata gattatccat tggcactaga
     8581 tgatatggat gttaagggta tttattatgt gaagaattat attgagagta tagatataga
     8641 aaatagattt tgtaacctat ttaagaaaaa agatagagaa aaattatttt atgactatgg
     8701 ggtgatttat aaaatagatt gtgatgttat acttgtaaat ttatttgaac ttattataaa
     8761 taattgtatt ttttctacta ttttaggaaa tgaagtgatt aatttagaaa tttcaaaata
     8821 tgagtatgaa tttttagaaa aacaatttaa aaatatgtac aataatatta ctgaagataa
     8881 agttataggc tctgaagaaa gtaatataat tgaattgatt gaagtagatg atagttttaa
     8941 cactgaagta gatagtaatt tggattataa tgattttgaa gaaattaaca gagaaattag
     9001 aataaataaa aaaataactt tactacttat ggaagcaatt gaagtattaa taaaaagatt
     9061 aagcatagaa gatgaagatg taataagata tattaaaaaa tatgaaaaga tatttattga
     9121 atctgtgcta agctcaataa aaagtaacac ttttaaaagt atggtaactg tgagtaaaaa
     9181 taaaaatgaa cacttagaag actatattgt tgatgatgaa agtaaattgg atgatgaatc
     9241 gtttagagag atattcaatg agatattaga tagtacatct ataattgaaa aaataagaat
     9301 aataaaggaa aatatcaatg ctaaaaaaga ctttatcgat attcttgaat ctgaatgttt
     9361 atttggagaa gagtatttaa tgttgtttgc atcattaagt gaattagaat tggcaatatt
     9421 gggtagtgta gtattttatg aagatattag aatgagagaa attaatatat tagaatttat
     9481 gttaaatgag aaaactgaaa ctactttatg gaagacagag tacattaaat ttatgaagag
     9541 gcaaagtgaa gataagatat actccataga agagcatatg aataaaatta attaacaaaa
     9601 aattgtgaat aaatagaatg ttagtatata atataatctg taaagcatat caaaaataca
     9661 tatatttgga ggaaaattat gaaagagtat atcataaata atgttgaaga aataagagaa
     9721 gaattaattg atttatctaa gaaaatatgg gaaaatccag aattagcctt tgaagaaaag
     9781 tatgcaagta gcattcaaaa agaataccta aagtcaaaag gatttaaaat tgaagaagta
     9841 gaaaatttac ctactggatt tatagcaagt tttggagagg gtaaacctgt gattggcata
     9901 cttggagaat atgatgcttt accagagcta tcacaatgtg tgagtgcaga aagaaagcca
     9961 ttggtagaag gaaaagctgg acatggatgt gggcataacc tgttgggtgt agcaggtgta
    10021 ggagcagttg tttctataaa aaaattaata gaagaaaata aatttaatgg aacaataaaa
    10081 tattttggtt gtcctgctga agaagaaggt ggaggaaaga cagttatgtg tataaatggc
    10141 tgttttgatg atgtagattg tgcatttaca tggcatcctt ttgatataaa tgcaccttgg
    10201 agaggtggaa gtctggcaaa cttatctgta aaatttaaat ttaaagggat aacagcccat
    10261 gcagctcaag ctcctcataa tggtagaagc gcacttgatg ctgttgaaat tatgaatgtt
    10321 ggagctaatt atttgagaga acatgttatt gactctataa gaatgcacta tgttataact
    10381 aatggtggtg gaagacctaa tgtagttcct ggatttgctg aaagttggta ctttattaga
    10441 ggtaaaaaag caagggatgc agaacatgtt cttgatagat taataaaagt agctcaagga
    10501 gcagcaatga tgacagaaac tgaaatggag tataaggtta cagatggaat atatgactat
    10561 ataccaaacc aatgtttgac tgatttagtt tatgacaata tggtttttgt tggttgccct
    10621 aaaaatactc ctgaagaaga agaatttgca aaaaaattgt gtgatactct tactagagaa
    10681 gaaagattag gtgtagcaac atcattacaa aatgataaat ctattgtaga aagttatata
    10741 cacaaggaaa tagttgacgg tgacaaagat aaaggactag ctggttctac tgatgttgga
    10801 gatgtaagtt atgtaatacc agtggctcaa tttgcaatgg cagcttggcc tgttggaatt
    10861 gcaagtcata catggcagtc atgttcatca gcaggttcaa atataggatt ttctgctatg
    10921 ataaattcag ctaaagtact ggcttgtagt gcttatgatg tatttatgaa tactaaaatt
    10981 attgatgaag caaaaattga atttgataaa tcattagatg gacaaaaatt taaaccattg
    11041 gtataatttt ggtaataaaa tatttctggg gggaatagct atgagagaaa aagttgaaaa
    11101 agtgcttgag gaaaagataa aaccagtatt acaaagagat ggtggagatg tagagcttat
    11161 agatgtaaat gaaaatggtg tggttttagt tagactacaa ggggcatgta gtgggtgtcc
    11221 aggagcgact atgacaatca aagctattat tgaaaatgtt ttggtgagtg aagttccagg
    11281 agtaactcaa gtattaggtg tttaatagtt agcgtttatt aaatgtatgt tatacataag
    11341 tatttaataa agaagtaaaa aaaatagtga agc
//
LOCUS       DAGQWR010000134        11314 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.135, whole genome shotgun sequence.
ACCESSION   DAGQWR010000134 DAGQWR010000000
VERSION     DAGQWR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.135"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(25..591)
                     /locus_tag="MIW95_003100"
     CDS             complement(25..591)
                     /locus_tag="MIW95_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBY3152406.1"
                     /translation="MDTKKVTFSSICIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            757..1278
                     /locus_tag="MIW95_003101"
     CDS             757..1278
                     /locus_tag="MIW95_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBY3152407.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFNEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            2069..4147
                     /locus_tag="MIW95_003102"
     CDS             2069..4147
                     /locus_tag="MIW95_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBY3152408.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            4147..4926
                     /locus_tag="MIW95_003103"
     CDS             4147..4926
                     /locus_tag="MIW95_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY3152409.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            4919..5728
                     /locus_tag="MIW95_003104"
     CDS             4919..5728
                     /locus_tag="MIW95_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBY3152410.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            5748..6230
                     /locus_tag="MIW95_003105"
     CDS             5748..6230
                     /locus_tag="MIW95_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3152411.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            6251..6655
                     /locus_tag="MIW95_003106"
     CDS             6251..6655
                     /locus_tag="MIW95_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY3152412.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANDDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(6811..7971)
                     /locus_tag="MIW95_003107"
     CDS             complement(6811..7971)
                     /locus_tag="MIW95_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBY3152413.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            8179..9729
                     /gene="xylB"
                     /locus_tag="MIW95_003108"
     CDS             8179..9729
                     /gene="xylB"
                     /locus_tag="MIW95_003108"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBY3152414.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNIIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYINTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYKIYHRLYKRIYKSLKDDFKLLDE
                     VVNTKINN"
     gene            9825..11162
                     /gene="xylA"
                     /locus_tag="MIW95_003109"
     CDS             9825..11162
                     /gene="xylA"
                     /locus_tag="MIW95_003109"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBY3152415.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNVTDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
BASE COUNT         4348 a         1308 c         2048 g         3610 t
ORIGIN      
        1 aaatcttaca taataaatga tttattaata tgaactagaa gtatttttac tatattgttt
       61 tataaaacct ttaggcattt tacttataaa atataccacc ataatacaag acaaaggttt
      121 gtctactata ttactaagaa tccttggaaa gaacgcagca gtaaatatct tctgaccaga
      181 ctgaactaaa aaaccaacca ataaatcagc accacttcca cttaaaccac caaacaataa
      241 aactgaaata ggagtaccaa ttaaaggaca aacaatagat aaaattactc ctgtaataat
      301 agcttcttta taaccaaaac cttttttctt agaaatatat ccaacaataa gaccaagaac
      361 aatatttaca agagcaaaag gtatatttgt aggacctgat ataacaccta aaactaagtt
      421 tgtacaccca ccaataagag caccccaaaa aggacctaat acgacagccc caagtacagt
      481 accaactgta tctaaaaata ataatggtat atttatcata gatataacca tacctaagac
      541 aatattaata actatgcata ttgaagaaaa tgtgactttt ttagtatcca attataatac
      601 ctcctaaaaa aatatataca tttatcatag tatagattat aaaagattac taattgtata
      661 tttctatata gaatattgga aatatagaac aaataaataa tataattatc aaaatatgta
      721 gtatcattaa aattaagaat aactaaggag attattatga agagaaatag agagattgta
      781 gtagtatgtc attgtatatt aaattgtaat tctaaagtag agggattgtc agaatttaag
      841 ggagcacaaa atatagcgat aaatttaata aataaagggt atggaataat tcaactacca
      901 tgcccagaaa tgattatgta tggaataaat aggtggggac atgtaaaaga acagtttgac
      961 aatttgtttt ttagagaaca atgtaggcaa atgttaatac ctcatgtaag gcagtttaat
     1021 gaatatttaa aaaatgatta tgatataaaa agtatcattg caatagatgg tagtccaagt
     1081 tgtggttata ataagacatg tagttctaaa cgttggtttg gtgagctaag tggttgcaat
     1141 gaattgatgg ataaaataaa tgatatacaa atggtggatg ggaaaggtat ttttatagaa
     1201 gagctagaaa agctacttaa agaatataat ttagagatac ctatagttgg atttgacgag
     1261 cttagagaag ataagtaaag atataaatat ttattgtctc attatgaaac accattatat
     1321 ttggcagaaa aatatgactg atggacagta cctgaatcat ttttaagtgg agtaattagt
     1381 acttataaag atgtatctac tagctgaaag aaaatgtaga gaggtaataa atgaattgat
     1441 aatatatata aaggagtgtc taaaagtgaa ctttttagtt tatgagacac tcttttttta
     1501 tattaaatca agtatattaa atcaaatata ttttaatcat tttagcaatg aaaaataaat
     1561 ttatctatat tttgagacaa catctttttt agttttattt atttgttgtc gtttttgact
     1621 tggtatacaa ttatatattt tcttaaataa tttcataaat tatttataat tataaaatcc
     1681 attgttttga acaatatcta atatattatt atctgtattt ataaggtcac tatacatatt
     1741 ttcaagtcca atattatcta cataataggc aaattttatt cctgtatgtg tttaaaaaaa
     1801 actagatgta tataggtatc atcacagata ttaaaagtat aaataaaaga tattaagaac
     1861 gactttcaag caacatatta gaaaatttta ctaaaatccg gaaagcgatt gacaaacaaa
     1921 gtcaaataaa gtatattatt ggtaagttag tttaatgtac aaatgaatta tatataaaac
     1981 ctaatttata aataacgata ctaaacgaca ttatttaact taagctacac aaagatagaa
     2041 tattatacca ttgatttagg aggtagccgt gataaaaata tctgaagact atattgaaaa
     2101 aagatttgac aatgaactat tgcgaataga agcatgggga aaaaactctt tacgtattag
     2161 gtcgtttgta gaccaaaatt ttgttgatga aaactatgct cttaacgaaa aacctaagtt
     2221 aaataaagat gatattacaa ttaataaaaa tgaagatgga agtgcaatca ttaaaaatgg
     2281 aaagataaaa gctgtcttag accatagaga tagaattact ttttataatg ataaaaatga
     2341 aatattattg aaagaatata ttcgattaag agctgtaaaa catgatgatg gtggagaaga
     2401 tgtagggaca attgagatta ccaaagactt taactctact ctaaagttaa aatcaagaga
     2461 atatcgacca agctatagtg gagaatttga agtaactact agatttgaat ctgagccttc
     2521 tgaaaagatt tttggaatgg gacaatatca acataaattt ttagatttaa agaatactgt
     2581 tttggagtta gcccaaagaa attctcaaat atcagttcct ttttatattt ctagtctagg
     2641 ttatggactt ttatggaata atccgggaat tggaaaagta tcttttgcta aaaatatgac
     2701 agaatggaag atgttttcta caaattttat agattattgg attacttgtg gagaaagccc
     2761 aaaagagtta aataaaaatt attctcaagt tactggaact gttcctatga tgcctgaaaa
     2821 ccttttagga ctatggcaaa gtaaactaag atatagaact tcagaagaag ttttagatgt
     2881 tgtgaaggaa tattctaaaa gaggaattaa gctttcatca attgcaattg actattttca
     2941 ttggcctaag caaggtgaat ataaatttga cttagattat tggaaaaatc ctaaagaatt
     3001 agttaaaaaa ttgaaagaag agtattcagt agaaccaata gtatctgtat ggcctacagt
     3061 tcaaagtgat gcagaaaact acaatgatta tttagaaaat ggctatttag tcaatgttaa
     3121 tcgtggtgtt agaatgacaa tgcaaattca aggaaataca gtctttgtag atatgacaaa
     3181 tgaaaatgca agagaatatg tttgggatag aatagataaa aattataaac aacttgggat
     3241 tgactattat tggttagatg tagctgaacc tggctattct gtgtatgatt ttgataacta
     3301 tagatataaa aaaggaaatg ttttaagttg tggtaatatt tatcctattg attatttaaa
     3361 aatgatttat gatggtttac ataatgacat agagtctgtt gtaactcttg taagaggagc
     3421 ttgggcagga gctcaaaagt atggagcttt agtatggtct ggtgacattg attctagttt
     3481 tgaggctttt aataatcaag taaatacagg gttaaatatg ggactggcag gtataccatg
     3541 gtggactact gatattggag gtttccatgg aggaaatcct aaagacccag aatttagaga
     3601 acttatggta agatggttcc aatatgctac tttttcacca attttgagaa tgcatggaga
     3661 tagattgcct catagtaaac cattatcaaa taaaggtgga ggctcaatgg taactggagc
     3721 tcctaatgaa atatggtctt atggtgaaga agttgaagta attcttacaa aattcataaa
     3781 aattagagag agcttaaaaa cttatttaaa aaaactgatg aaagaagcgc atgaagatgg
     3841 aacaccagtt atgagaactt tattttatga atttcctgaa gatgataaaa cttgggaggt
     3901 ggataataca tatatgcttg gagatgaaat tttggtagct ccaataatga attataaaga
     3961 tagaagtaga aaagtatatt taccaaaagg acatacatgg gaaaatatat ttagtggagt
     4021 tagttatgaa ggtgggaaaa cttatgaagt tgaatgcccg ctggaagaaa ttcctatttt
     4081 cctaaagcaa gattccagtt ataattttaa agaactaaaa aatattttgg gagaggtgaa
     4141 atcataatgg agccacaatt atggcaatta ttattaatcg tactatatgg attttttata
     4201 aactatgaaa aaaattcaac aatgtttgga acatatcaac cagtaacagc aggttttatt
     4261 actggtctta tcttaggaga tataaataca gggttatata ttggaggtac attacagcta
     4321 ttatcattag gtattagtaa ctttggtgga gcatcaatac ctgattatca aacagcaagt
     4381 atagtagcca catttataac tattacgact aagcaagaag catctgtagg aatctctata
     4441 ggtattccag ttgctttatt gatggtacaa ttagatgttc ttagaaatac tattggtata
     4501 tggctagttc ataaagcaga agatggagct aaaaaaggaa attataaaaa tataacctat
     4561 atgcaaatgt taggagtact attaacagca gctacaacag gtattccagt tgctctatct
     4621 gtaatatttg gaccttcttt aattaatacc attttgaaat atacacctga atggttaaca
     4681 ggtggtttaa cagtagctgg aggactactt ccagcagtag ggataggatt attacttaga
     4741 tatcttcctg ctaaggagta ttttagttac ttagtaattg gttttgtttt agcagtatat
     4801 atgaaagtac cattattagg tgtagcttta attggtggag caattgcact tataatttac
     4861 aaaaagaatt tggaaaatca agaacaacaa tatactgtag taggaggtat ggacgaagat
     4921 gagtgataag gtatcattaa ataaatcaaa tgaaaaggtg aaaaaaagtg tcttatggcg
     4981 ctggtttttt actagttctg tttcttcaaa ttatgagaag atgcaggcct tggcatactg
     5041 ttatgcagta ctaccttttt taaaagtagt atacaaagat aatccagaaa agttacaaag
     5101 tgctgtatta aatcatttac aattttttaa cactaatcct tgggttgccc cttatatttt
     5161 gggtattaat gtagcaatgg aggagttatc aagtgaagag actgaagagg ctgttgcttc
     5221 aattaaaaca ggcttgatgg gaccagtagc aggtttagga gatagtttat ttgtcgttat
     5281 tccgtggaca atttttggag ctatagcagc taatatggcg atagatggaa gtccatttgg
     5341 aatcttactg tggattgtag tgagtgtagc attgaaatta ttaagtatac cactatttaa
     5401 tgctggctat acatcaggta ctaagctaat atcttcaatt gaaaaaagtt taaagatttt
     5461 aactgagagt acatctattt taggtttaat ggttgtaggt gcattaatac cttcagttgt
     5521 aaaagctaat gttgctttga actttcaaca aggtgatttt acaatgaaag gtcaagaaat
     5581 attagaccaa attatgcctg ggttagttcc tgcaatttta gttgtgattg tttattgggc
     5641 tcttaaaaag aatataaaac ctatatactt aattttagga gtaatggttg tttcaataat
     5701 tttatatgca ttaggtattt taaaataagg aaaggatgat attagatatg gaaatgaaag
     5761 gtattaaaaa tattcgtata gatgaccgtt taatccatgg tcaagttgca actatgtgga
     5821 gtaataaatt gggtgttaca agattaatgg tagtaaatga tgcagtagct aacaattctg
     5881 ttcaaaaaca agttttaaga atggctacac ctgcaggtat agcgtcatct ataattacag
     5941 aagagactgc tataaaaaat attactgcag gaaagtatga aggacaaaat gtattattaa
     6001 ttgttaagtc tcctgtggac cttattccat ttgtagaggc aggatttaaa atacataata
     6061 taaatgtagg aaatatgtca agtagaaaag atacaacagt tctaagacct aatatcagtg
     6121 ttactgaaga agagaaagaa gcatttaaaa gacttttaga tgaaggaatt gaaattacaa
     6181 ctataatgac gcctgatgat aagaaaactt atcttgtaga catcttatag atttaaagag
     6241 gtgtattaaa gtgaatagta ttattttgat gagtcatggc aatatggctg aagaagtctt
     6301 aaattctgca aaaatgataa taggggattc tatagattat cctactgtaa atatgaaaca
     6361 agatgatgga atagaaggaa caatagaaaa attaaaaaaa gctttagaag tgtacaagga
     6421 aagttcagaa ataattataa tggcagattt actaggtgga actccatgca atgctgcttt
     6481 attagtagcc aatgatgaca gtcgaattaa aattatttca gggctaaatt taggtatggt
     6541 tattgaatca gcttttttag atacaaagga attggcagca aaattagaga aaataggtaa
     6601 agataatatt tctatggttg aacaaaatgc aagtttatta tctgatgatg agtaggaaga
     6661 ttatattaat atttaaaaca aaatacttgt gttaatactt aattgatata caatttttaa
     6721 aaaatatagc taatcttgca taaactatat aataaatcta attttagaat gtataattta
     6781 aagggttatg gctgtatata gtcataaccc ttatatttca ttaattaaag ttaaattttg
     6841 tatattcaaa aagctcttga tatttacggc agagccacca tatagagatg cctcagaccc
     6901 taatgatgaa atttctatat ttattccttt gttaaaagag ctaactaaca tatcttttgt
     6961 aatctgtaat atctcaggga tatcactaat tatttggcta tttaaatata ttatttcagg
     7021 agcataagtt gtaatagcat tatttatagc tattgttaag taactacaaa actcatgaat
     7081 aacttttttg gcattctggt tatcttcata atatagttgc tttactatat cagaatctat
     7141 tttaggaata ttttctaaag aagaaagctg ttcaaatacc tttttttctg aacaatactg
     7201 ttctaaacag ccacgatttc cacaaggaca tagtttacca ttaggcatga tgatagtgtg
     7261 accaatttct ccactcatac catttctgcc gctatataat ttattattta ttataatgcc
     7321 tgaaccaaat ccactgtgaa tactgagact aagtagtgag ttatgtacag ttgaaaaagt
     7381 attctcagct aaagctgtta agtttgcttc attttctata tgaataggaa agtcatactt
     7441 tttgctaagg atgctatata agtcaatctc attaagatta taatatggag taaacaatac
     7501 tttattttca caagtaatcc catgtatagc caaagtaaga ccaattacct tataaggagt
     7561 gtctattttg gatatgttat aactatttat aatttcatct atcaattgta taacattgtc
     7621 tttacttact tgtatatctg ttaatttctt agaatttata atagttccat ctaagtaaga
     7681 tagagaagaa aatatatagt cgtatccaat gtccatactt aaagaaatcc ctgcacattt
     7741 attaaatact aataatatgg gttttcttcc gccactatga gtactatttc caattcctat
     7801 ttcatgaact agagattcat caataagttt tttggtaata gcggatatag ttgctttatt
     7861 caatcctata gtagaagcta tacttgccct agaaatagga ccatttttta taatttgttc
     7921 aagtaccaat ctttcattca tttctcgaat agtgtattta tcagtaacca atttgatatt
     7981 cctccttaaa ataatattgt aatacttttt acacaaaaat aaaaggttat tttgcattga
     8041 caaagataat taaatatttt attattagtt cataagttag tttaatatac taacaaaaat
     8101 aaagcaagta aaatatacct aaaatataaa aaaattagga taggaaaacg atagttatga
     8161 agtggcattc aaggagggat gaagttggag tatgtattag gtgtggatat tggtacatca
     8221 ggtactaaaa cagttttatt tgataaacta ggaaatatta taaaaagttg tacatatgaa
     8281 tatcctctta tacaagagaa atcaggttgg gcagagcagg atgcaaatga ttggtggaaa
     8341 gctgtagtcg aatctataag agaagtagtt caaagttcaa atataagcag tgaatgtata
     8401 aaaggaattg gattatcagg gcagatgcat ggtctagtta tgctggacaa tgaaggtaaa
     8461 acattgagaa actctataat atggtgtgac caacgaacag taaaagagtg tgaggagata
     8521 actgatttag taggtgaaga aagactgatt gaaataactg ctaatccagc gcttacagga
     8581 tttacagcat caaagatatt atgggttaga aacaatgaac cagatatata tataaatact
     8641 aataaaatac ttcttccaaa agattatata agatacaaat tgacaggtga atatgctaca
     8701 gaggtatcag atgcaagtgg tatgcagttg ctcgatataa gaaaaagaga ttggagcgat
     8761 gaagtactag aaaagttaaa tatagataaa aatcttttag gtaaagttta tgaatcacaa
     8821 gagattacag gttatgtgac tagagatgta gcaagtttga cagggcttaa agaaggaaca
     8881 atagtggtag gaggagctgg agaccaagca gcaggagcta taggaaatgg aatagttaaa
     8941 gatggagtag tttcatctac tataggtaca tcaggtgtag tatttgctta tacgaaagag
     9001 ccaaaaattg ataaagaagg tagagttcat actttctgtc atgcaatacc aaatacatgg
     9061 catgttatgg gagttactca aggagctgga ttgtcattaa aatggtttaa agataatttc
     9121 tgtcaaagtg aagtggaagt ttcaaattct ttaggtgaag atgtatatga gattataaat
     9181 aatcaagtaa gtcaagtacc aacaggatgt aatggattgc tttacttacc atatatgatg
     9241 ggagaaagaa ctcctcatct tgacccttat gcaagaggtg tgttctttgg attatctcca
     9301 atccattcta aaaaagaaat cgcaaggtca ataatggaag gtgtttcata tagtcttaaa
     9361 gactgtatgg atataataga gaatcttaat atagacgtta atgaagtaag agcctcaggt
     9421 ggaggtggaa aaagtaaagt atggagacag atgcaagcag atatgtttaa tcaagatgta
     9481 tatactataa attcaagtga aggaccagca cttggagttg caattcttgc attagtagga
     9541 gctggaatat atgaaaatat acaaaaagct tgtgatgcta taataaaaac ttcaactaaa
     9601 ttagaaccaa taagtgaaaa tgtagataca tacaagatat atcatagatt gtataaaaga
     9661 atatacaaat ctttgaaaga tgactttaag ttattagatg aagtagttaa tacaaaaata
     9721 aataattaaa ttagcttaaa aagaaaaata aagatagaaa atagttttaa attaagaaaa
     9781 catacgacat atattatatg tgtaaaatat taggaggaca tgaaatgagt gaaatattta
     9841 aaggaatagg acagataaaa tttgaaggag taaaatctga caatgaatta gcatttagat
     9901 actacaatcc tgaacaagtg gtaggtaata agactatgaa agaacactta agatttgcaa
     9961 tgagctattg gcatacttta tgtggagagg gtaatgaccc atttggagta ggcacagtag
    10021 agagaccttg gaataatgta acagacccaa ttgagatagc aaagattaaa gttgatgcag
    10081 gatttgaatt tatgtctaag atgggaattg agtatttctg ttttcatgac agagatatag
    10141 ctccagaagg aagagattta gaagagacaa ataagatatt agatgaaata gttgagtata
    10201 taaaagttaa tatggaaaag acaggtataa agttattatg gggaactgcc aatatgtttg
    10261 gaaatccaag atttgttcat ggtgcatcta caacatgtaa tgcagatgta tatgcatatg
    10321 cagcagctca ggttaaaaaa gctatggaaa taactaagta cttaggagga gaaaattttg
    10381 tattctgggg tggaagagaa ggttatgaaa ctttacttaa cactaataca gaacttgaaa
    10441 tggataactt tgcaagattc ttacaaatgg ctgtagatta tgctaaggaa ataggattca
    10501 ctggccaatt cttaatagag cctaagccaa aagagcctac taaacatcaa tacgattttg
    10561 atactgctac tgttttagga tttttaagaa agtataatct ggataaatac ttcaaaatga
    10621 atatagaagc aaaccatgca acacttgcag gacatacttt ccaacatgaa ttaaatatag
    10681 caagaataaa caatgtttta ggtagtatag atgcaaatca aggagaccta ttattaggtt
    10741 gggatacaga ccaatttcca acaaatatat atgatgcaac acttgctatg tatgaagtgt
    10801 taaagcaagg tggaatagca ccaggaggat ttaactttga ctctaaagta agaagagcat
    10861 cttttgaggt agaagactta ttcctagctt atattgcagg aatggataca tttgcaaagg
    10921 gattattaat agctcataaa ttattagaag atgaggtatt tgaaaacttt actaaagaaa
    10981 gatatgcaag ttttagtgaa ggtataggta aggatatagt agaaggtaag gttggattta
    11041 aagagttaga atcttatgca ctgcaaatgc cagttataaa gaataagtct ggaagacaag
    11101 aaatgctaga agctatactt aacagatata tatatgaagt agatacaatt tctaataagt
    11161 aattgtgtag taattaaact tggatgtttg aataaaataa caggatatgt tcttgttttt
    11221 attctaaaac ggacaaagat aagtatttgt aaaaattctc ataatcatga taaattaagg
    11281 atatagactt aagtataatt agtttatatc ctta
//
LOCUS       DAGQWR010000135        11184 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.136, whole genome shotgun sequence.
ACCESSION   DAGQWR010000135 DAGQWR010000000
VERSION     DAGQWR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.136"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            92..637
                     /locus_tag="MIW95_003110"
     CDS             92..637
                     /locus_tag="MIW95_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBY3152416.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            650..1663
                     /locus_tag="MIW95_003111"
     CDS             650..1663
                     /locus_tag="MIW95_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBY3152417.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1885..3858
                     /locus_tag="MIW95_003112"
     CDS             1885..3858
                     /locus_tag="MIW95_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBY3152418.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKENNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            3927..4286
                     /locus_tag="MIW95_003113"
     CDS             3927..4286
                     /locus_tag="MIW95_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152419.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4321..4677
                     /locus_tag="MIW95_003114"
     CDS             4321..4677
                     /locus_tag="MIW95_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBY3152420.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4714..6006
                     /locus_tag="MIW95_003115"
     CDS             4714..6006
                     /locus_tag="MIW95_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBY3152421.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            6442..7080
                     /locus_tag="MIW95_003116"
     CDS             6442..7080
                     /locus_tag="MIW95_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3152422.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            7087..7929
                     /locus_tag="MIW95_003117"
     CDS             7087..7929
                     /locus_tag="MIW95_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBY3152423.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            7990..10617
                     /locus_tag="MIW95_003118"
     CDS             7990..10617
                     /locus_tag="MIW95_003118"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBY3152424.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            10831..11076
                     /locus_tag="MIW95_003119"
     CDS             10831..11076
                     /locus_tag="MIW95_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY3152425.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
BASE COUNT         4450 a         1142 c         2161 g         3431 t
ORIGIN      
        1 catatagtac agaaaaaaga tgtaatttgt aaaatttatg ttaagcagta aaattaaaaa
       61 tttaaagata ttatagggag gttataattt tatggaaaac gttattacag gtctaagtaa
      121 aggtgcagag tggtttatcg gtttgtttca gaaaggggga gaacaattta taggtcttgt
      181 ttcaggtact cttccaacgc taattgtatt aatggtagct attaactctt tgattaagat
      241 aattggtgag gaaagagtta ataattgggc ttctaagcta ggaaagaact cttttacaag
      301 atatattcta cttccattag tatcagtatt tttcttggga aatcctatgt gctatacatt
      361 tggtagattt ttaaaagaag agcacaaagc aggattttat gatgcagcag tttcatttgt
      421 acatccaatt acaggtcttt ttccacatgc aaatgcagga gaactttttg tatggttggg
      481 aatatcagca ggtttgacta ctttaggtaa agaaactact acactggcat tgtggtattt
      541 tatagttggt ctaattgtaa tttttataag aggtatagta acagaaaaaa tgtatgcttt
      601 tctaacaaga aaaaatagag caacaaataa ggcttaggag gtagataaaa tggaggagaa
      661 taaaatatta aaaatagaaa agggaacttc aggctgggga ggtcctttgt atataaaaaa
      721 agaagggaat agaaataaga tactatcaat gactgctggt ggtatccatg aagtaacatt
      781 aaaaataaaa gaactactgg gttgtgaaat tgtagatgga tttaaaactg gagtttctga
      841 tgaagaagta gcagtagtta ttattgattg tggtggaact gctagatgtg gagtttatcc
      901 aaagaaaaaa atacctacaa taaatgtaaa tccagttgga aaaacaggtc ctttagcaaa
      961 atttataaca gaagaatatt atgtatcaga tgttaatcct aactgtataa gtgttgtaga
     1021 tggggaagat atgccacaaa aatctcaaga aaataaaagc gaaaataaga gttctattag
     1081 aaagcctgat aattatgatg aggttaagtc aaaagcacaa ggtgaatatg ctaaaaagaa
     1141 cataatactt tctattggtc aaggggcagg gcaagtagta tcaaaatttt atgatgcagg
     1201 tcgagacact atccaaatgg taatgaataa cgtaatacca tttatggcat ttgtaagtat
     1261 gcttatggga attattttag catctggact gggagactgg attgcaagag ttatatctcc
     1321 acttgcaggt aatattggtg gattattgat tatatcagta atttgcacac ttccattctt
     1381 atcaccaata ctaggacctg gtgcagttat agctcaagta gtagggactc ttgtaggtac
     1441 acaaattggt cttggtgcaa taccagcata tttagcactt ccagctttat ttgcaataaa
     1501 tgggcaagct ggatgtgatt ttgtaccagt aggattaagt ttgggagaag cagaaccaga
     1561 aactgtagag tatggtgttc cagctttatt ttactctcgt ttaataactg gtccaatagc
     1621 tgttattatt gcatatggag ttgcagtatt tgcattgaga taatatttaa tgtcaatata
     1681 aggcttggaa ttaattaata aaattgtaaa tataaattta agctagtcaa gattttttgt
     1741 taatgaaaga ctagctttta tactattaat tagctgtaat tatcgagtta gattaatact
     1801 aaattttcct ttattaaagt gaaattttat gtattaattt ggtttcaaat ttgtactaga
     1861 atgaaatcaa ggaggagata caaaatgagt gtaactgaga aaattccttc tattgaccgc
     1921 aagatattta agatattgat tatgtgtagt aaaaaagagt ttgtgagtat taattcaata
     1981 gcaaatgaat tgaatgtcac aactagaagt gtaaggacgt atataaaaca attgaataaa
     2041 gatttaggta atgatatagc agtaatcaaa tatataaagg gtcaaggcta taaactagaa
     2101 ataaaagatg agcagatact caataagatt attgatatga atagaaaaaa tgtattttca
     2161 ttaaattcaa aagaagatag agtagagttt atactaaatt acttgataga actagatgga
     2221 tttattacac ttgatagttt agctgatgag atgtgtgtag gaagaactac tttagtaaat
     2281 gatttccagt atgtagaaaa agttttggct tcatataatt taaacttgat taaaaaacag
     2341 aatacaggta tgaaattaaa tggaaatgaa cttgatatta gactgtttat attaaatcag
     2401 ttgtataaaa actcaaggaa agatttcagt aattcaaagt attttaaagg aatcaaaaaa
     2461 gaagaaatca taaatttaga agaaaaacta ttgactttat ttaaagaaaa taatttttat
     2521 gttacagatg aaatgttaag agaagttatt aactacatag tagttttagt ctatagagta
     2581 aaagaagaaa agaaagtaaa agactatgat gtaaagtttg atttacttaa atcatatgat
     2641 gaatacttta ttgctaggga aataaaacat ataatatcag gaatgtttga atgtacttta
     2701 aatgatgaag aaattattta tttaactata ccattagtta gtggaaatgc accagcatca
     2761 gaatgtgctt taaatagtag tagaattagt aaaaacattg atgagttgat ggaaaatata
     2821 tttaatcaaa tatatgtcga tatgggtatt tttataaatg aggatgaatt aagagtaggt
     2881 ctaggatatc atcttagttt tactttaaac aggctattat ttaatataaa gttaaaaaat
     2941 gttttgttag aagagattaa gcaaaattat atacttccat ttaaattagc acaaatagca
     3001 ggaaaagtaa tagagaaaaa gtataactta gaagtttcag aagatgagat tggatatata
     3061 gcaatccatt tcagtggata tttagaaaga aatagcagta gattctattc aataaaaaaa
     3121 atagcaatta tatgtagtac aggtctagga acagcaaaac tattgaaaat aagagtagaa
     3181 aaactgatag gaaataatcc taaaattgat actctttcaa gttttaattt gagaaatatt
     3241 aacttagatg aatacgatat agtttttaca actattgatt tagacacatc gaatataaat
     3301 acaatagtat taaaaataaa tacaatattt gatgaaaaca aattaagaga acaattaaaa
     3361 acagtattgt gcctaagaga agggaatatt gacactacta attctacaaa tttactaata
     3421 aataatttgt tagatgaaga taaatttatg atattaaatg aaaagactat acttgcttca
     3481 ttagaaaaaa tgatggacca tcttatggat ttgggatgta ttgatgataa gtttagaaaa
     3541 aatatagtca atagagaaga aaaatctccc acagtatttg ataaaggttt attatttcct
     3601 cactctgtaa atgaaaaatc agataagttt ttaatggcag ttggaatttt agaagaacca
     3661 atagaatatg caaatagaag tataaaaata attcttatga caatgtttcc atgtgagaat
     3721 aagatagact cagacttatt agtaaaaatt tatgaagaag ttttaaagat aggccaagat
     3781 gtaaaattaa cgaataagat aagtaaatgt agaacttttt tagaatttaa aaaggtgtta
     3841 cttaaaaacc ttatataact attgtacagc atttatagtt gttatacaga tttaaaaata
     3901 atgtgatttt tggaaggtgt ataaatatga aattattagt aattttattt gttgcttata
     3961 ttttaaatac tatatttata atgaagcaga ataaattgta ttttaaaact ttaaatagag
     4021 ctaaaaaaaa gggtgatata gtgtctacag gcaaaaagaa aagctatttt tctaaaggaa
     4081 gtatagccat tatatcttca gattcagatg ggtttataaa gtttggagaa attttaaaag
     4141 ggcgtacagt aatggctaaa tttaaagaga ttgaagaaat agaaggatta gatatatata
     4201 gtgcagagca aaaatttaaa gatgaagaga gtattgtaca agctataaaa tttataaaag
     4261 aaaaaataaa tgtttctttt aattaactca atataaatat aaattatcag gaggaatgtc
     4321 atgaaatatg aagttaagat aagtgggata ggcagtttag ttaaagagtt aatggatgaa
     4381 agcaattgtc ttattatata tgatgaaacc attaatgatg acgacttaaa ggatatatct
     4441 gtaattcatt cgatagcaac attaaaaagt gatgttgaaa taggagatac acttactata
     4501 gggaatagag attattgtat agtttcagtt ggtgatattg ctcagaagac tcttagagaa
     4561 attgggcatt gtacaatcaa atttgatgga aaatgtgaag taaatttacc aggagaaata
     4621 catgtggaaa taggaaatcc agatataact attggagatt taataactat ttcttaattt
     4681 gaaaataaaa ttattataac aggaggaatt ggtatgttag atttaaacag aaaacttgca
     4741 aaattggaag aggaaggtaa aaaaataaaa gtagctctta taggagctgg gcatatggga
     4801 aatggaatgg taagccaaat ggcaaatatg aaaggtattg aagcttctat tgttgtagat
     4861 ataaatttgg agttggctca taaagctttt actgatgcag gaatagatga agaaattata
     4921 gataatgtaa caaatgaaaa ggatgcagaa ataaaattac aagaaggaaa agtattgaca
     4981 tgtaatgact ttcttgtggc ttgtaaaact aagtcaatag atgtagttat agatgcaact
     5041 gggggtattg caattggtgc agagatagca ttaaactcta ttttaaataa aaaacatata
     5101 gttatgttaa atgctgaaac tgattgtgtt gttggaccaa tacttaaaaa attagcagat
     5161 gatgctggag tgatatttac aggttctgca ggagatgagc caggtgcagt aatggaatta
     5221 tttgattttg cagatgcaat ggggtttgaa gttagagtga taggtaaagg taaaaataat
     5281 aaattagatt tagattgtaa tcctgataca gttagagaag aagcagaaag aaaaggagct
     5341 tcacctcata tgatagcttc ttttaaagaa ggaactaaaa caatggtaga aatggcttta
     5401 atgtgtaatg caactggatt tgtgcctgat gtaagaggtg gtcatggtat tgaagctaca
     5461 gttaatgaag tacctaagaa atatgcatta aaaagcgaag gtggagtatt agataattat
     5521 ggtgtagttg attttgtaaa tggtatagca cctggagttt tcgtagtagt agcccataaa
     5581 ttaaaagcag ttaatgatga actaaaatat ttaagtatgg gtgatggacc taattacata
     5641 ttatatagac catatcattt atgtagttta gagactcctt tatcagcagc tatggcagtc
     5701 ttagaccata aggctacaat agtacctaaa gctggactag tagcagaagt tatgactata
     5761 gctaagaaag atttgaaaaa aggtgagttt atggatggtt atggagcata tacatgctat
     5821 ggtactatag aaaaatacga tgtagcaaag gctatgaatg ctgtacctat tggtttgata
     5881 agtaaaaaga ctaaagttgt aaaagatatt aaaaaaggtg aagttataac ttacgatatg
     5941 gtagagatag aaaaagatac tactttatat catttaagac aattgcaaga aaaaatattt
     6001 gggtaagatt tgatggtaat ataaatagat attagtaaaa agggttgatg gttattttta
     6061 aatcaaccct tttaaatttt tatatattct tgttaggata ctttctttaa aagtatatta
     6121 taggttattt gtagtcgtaa aaattatttt gaattagttc tgcaatttta tattcacatt
     6181 gaaacaatat tattaaaaat tttaatataa aatgaattaa aatgaccaat tatgtattat
     6241 aatattaact gtaatactaa tatagttcta ttaattaaag aaatatcccc cattaatata
     6301 taataaatat ctttaagcga gagaacttag taaaaaatat atcgtattca ataaaaaatc
     6361 tgtttacaga atggtatata tagttatata atatactata taagatgcga aaagataaaa
     6421 agtatagcac aatgaaagaa ggtgattatt attcagctta atgaaagaca actaaaaatt
     6481 atagatatag ttaaggaaaa tgaaccaata acgagtgaaa gtattgcctc cagcttgaat
     6541 gtgactcgtg ctacacttag atctgattta gctatattaa caatgacagg aatattagac
     6601 gcaagaccaa aagttggata tttttattca ggagttagtg aaataaattt aattggtaaa
     6661 agtgtaaaag aaaaaacagt agaggatata atgagcatgc ctgtactagc taagaaggat
     6721 gaaagtatat atgatgtaat agtcactatg tttttatctg atgtagggag tatagttatt
     6781 atagatgaga atgaagagtt atgtggagtt gtttccagaa aagacttgct aaaagcaact
     6841 ataggaggtt ctgatataaa taaaatgcca ataggtatga taatgacaag aactccaaat
     6901 gtagtcactt taactaaagg agcaagtgtt ttattagctt caagaaaaat aatagaacat
     6961 gaagtagatt cgattccgat tgttgaatat aagggagaag acaaaaatca tatgagagtt
     7021 gttgggagaa tatcaaaaac caacataact aagctattct tagaaatagt tgacaattaa
     7081 ggaggtatgc tggtgaaaaa cctaattata tatgcagtat cagattcagt aggggagact
     7141 gcacaacaag ttgcaaaggc atgtatgtct cagttttatg tgaatgaaac ttatgaaata
     7201 aaaagatttc catacatgat taataaagga gtactacttg aaactttgga gaatgcaaaa
     7261 gctgaaaacg ctttaattgt atatacatta gttgatgaag aattatgcag tattgttgaa
     7321 agatattgcg aaagagaagg tcttagttgt atagatttaa tgacagatat actaagagaa
     7381 ataagcaaga gaacaggaag aaaacctaaa agagaagctg gtataattag aaaattagat
     7441 gaatcttact ttaagagagt tgaagctata gaatttgcag tgaaatatga tgatggaaaa
     7501 gaccctagag gtgttcttca agcagacata attttagtgg gaatatcaag aacatctaaa
     7561 actcctttga gtatgtattt agctaataaa aatataaaag tagctaatgt tccattagtt
     7621 ccagaaatac caattcctaa agaagtgttt gagatagata ctaaaaagat aataggtctt
     7681 actaattcac ctgaaaagct gaatgagata agaacccaaa gattaaaagc tttaggattg
     7741 tccagtaaag caaactacgc taatttagaa agaatacttc aggagttaga ttattcagaa
     7801 gagataatga aaagaattgg atgcccagta ataaacgttt caaataaggc aatagaagaa
     7861 acagcaggta taattttaga cataatgaaa gaaaatggtc taaagatata caaagaaata
     7921 gaaatttaat aatgtctatt aataaaaatg atataaatta aaataaaaag attaatgggg
     7981 gatttaacaa tggaaactaa gtatgtttat agttttggtg aaggaagcaa agacatgaaa
     8041 tctttactag gaggtaaagg tgccaattta gcagaaatga ctaagatagg cttacctgtc
     8101 cctcctggat tcactataac aacagaagca tgtaatgatt attatgttaa caatgaaagt
     8161 ataagagcag agataataaa agaaatagaa gagcatttag ctactttaga aaaagactta
     8221 aataaaacat taggatgcaa taagaatcca ttattagtat cagttcgttc tggtgcagta
     8281 ttctctatgc caggaatgat ggatacaatt cttaacctag gtcttaatga caatagtgtt
     8341 gttggcttag cagaggctac acaaaatgaa agatttgcat atgatagtta cagaagattc
     8401 atacaaatgt tttctgatgt tgctatggaa gttccaaaat ataaatttga aaatgtatta
     8461 gacagagtaa aagaagcaaa gggatacaca gttgatacag aacttacaac tgatgattta
     8521 aaagaaatag taaaagaatt taaagcaatt tataaaaaag aaataaaaaa tgatttccca
     8581 caagacccaa aagagcaatt aatgcttgca atagaggctg tatttagatc atggaataat
     8641 cctcgtgcaa tcgtataccg taaattgaat gatatagctc ataacttagg aacagcagta
     8701 aatattcaat caatggtatt tggtaatatg ggagaaacta gtggtacagg tgttgcattt
     8761 actagaaatc cagctacagg agaaaataaa ttatttggtg aatttttaat gaatgcacaa
     8821 ggtgaagacg ttgttgcagg tataagaact cctcaaaata tatcaactct tgcagatgta
     8881 atgccagctg tattcgatga atttgtaaaa attactcata tacttgaagg tcattataaa
     8941 gatatgcaag atatagagtt tactatagaa aatgaaagat tatatatact acaaactaga
     9001 aatggtaaga gaacagcagc agcagcaata aatgtagcgg ttgatttagt tgaagctgga
     9061 ataattgatg aaaaagaagc tatcatgaga attgagccta atcaattaga ccaattatta
     9121 catccaaaat ttgaagataa ggcattaaaa gaagccaagg ttatagcaaa aggtttacca
     9181 gcatcaccag gtgcagcaag tggtaaagtt tatttcaatg ctgatgatgt agtaaaagca
     9241 aatgaaaaag gagaaaaagt agttttagtt agattagaga catctccaga agatatagaa
     9301 ggaatggtta aagcagaagg aatccttaca gctagaggtg gaatgacatc acatgcagca
     9361 gttgttgcta gaggtatggg taaatgttgt gttgctggat gtggagaaat aaaagttgat
     9421 gagtttaata aagaaataag agcacttgat gatgtagtta taaaagaagg tgaatacatt
     9481 tctatagatg gttctacagg aaatgtgtac ctaggagatg ttaagaaaac agaagtatct
     9541 ttaactggaa actttgaaaa attaatgaac tgggtagaca aacacaaatg tatgatggtt
     9601 agaactaatg cagataatcc tagagatgct cgtgcagcta tagagtttgg tgcagaaggt
     9661 ataggtctat gtagaactga gcatatgttc tttgatgaag ctagattgcc agcagttaga
     9721 gaaatgatat tatctaatac agtagaacaa agagaaaaag ctcttgaaaa aatattacca
     9781 atgcaaagag aagattttgt agagttattt aaagttatgg atggaaaacc agttaatata
     9841 agattactag acccaccact acatgaattt ttgcctcatg atgatgagac tatagaagaa
     9901 ctttcaaaat ctatgggaat aaaagtaagt gatattaaga agagaatagt tgatttagat
     9961 gagtttaacc caatgcttgg acacagaggt tgtagacttg caataactta tcctgaaata
    10021 tgtgtaatgc aatctaaagc tataatacaa ggtgctatag aagcaatcaa agcaggtgtt
    10081 aaagtttctc cagagataat ggttccatta gttggagaag taaatgagtt aaaaataata
    10141 agaaaaatga tagtagaaac tgttgacgct ataataaaag aagaaggtat agaagttcct
    10201 tatactgtag gtactatgat agaaatacct agagcttgtt taactgctga tgaaatagca
    10261 caagaagcag acttcttcag ttttggtact aatgacttaa ctcaaatggc atttggatat
    10321 tcaagagatg atgctggtaa attcttaggt caatatgtag atgaagaaat attagaaaaa
    10381 gacccattcc aagtactaga ccaaaatggt gttggtaagt tagttaagat gggagctaaa
    10441 ttaggtagag aagttaaacc agaattaaaa ctaggaatat gtggagagca tggaggagaa
    10501 ccatcttcag tagagttctg ttatagtgta ggacttaact atgtatcttg ttcaccattt
    10561 agagtgccaa ttgctagact tgcagcagct caagcatcta taaaaaatcc tagataataa
    10621 tagactgcaa aatatgtaac taaataaaaa taaaatcccc tattgttcaa tgtatcaaat
    10681 taagtatttg taaattgaat ataggggatt tttattataa aaatttaact tatacagttg
    10741 taagtgaaac gtatttaatt atattgcata tattattgta gagagaaatt tcaactatca
    10801 attaaattga gtttactgga ggggataaag ttgacaaagt gtcctaaatg tggaaaggaa
    10861 gtatcatcac gtccaggtag tatatgccca cgatgtggat ttaaagtagc agatgagagt
    10921 ctaaaaataa gatgtccaga acctagttgt agggctttgg tatctaggaa actggattat
    10981 tgtcctaaat gtggatgcaa acttagagga tataatattt ctgaatttgt taacatggta
    11041 agatgtaaaa tagatgagac atttgataaa aaataattaa atttagagtt aagcttttga
    11101 gggtgtctca aaatgaactt tttagttcag aaggcactct ttttgtatgg aatcaaatat
    11161 atttttataa tttaaacaat aaaa
//
LOCUS       DAGQWR010000136        11104 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.137, whole genome shotgun sequence.
ACCESSION   DAGQWR010000136 DAGQWR010000000
VERSION     DAGQWR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11104
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.137"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            487..639
                     /locus_tag="MIW95_003120"
     CDS             487..639
                     /locus_tag="MIW95_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152426.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHYPAFAGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(931..1635)
                     /locus_tag="MIW95_003121"
     CDS             complement(931..1635)
                     /locus_tag="MIW95_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3152427.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1626..3233)
                     /locus_tag="MIW95_003122"
     CDS             complement(1626..3233)
                     /locus_tag="MIW95_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBY3152428.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3487..4665)
                     /locus_tag="MIW95_003123"
     CDS             complement(3487..4665)
                     /locus_tag="MIW95_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152429.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4891..5385)
                     /locus_tag="MIW95_003124"
     CDS             complement(4891..5385)
                     /locus_tag="MIW95_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBY3152430.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5386..6147)
                     /locus_tag="MIW95_003125"
     CDS             complement(5386..6147)
                     /locus_tag="MIW95_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBY3152431.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            6870..9704
                     /locus_tag="MIW95_003126"
     CDS             6870..9704
                     /locus_tag="MIW95_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3152432.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHVSIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNISKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNIRKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            9747..10991
                     /locus_tag="MIW95_003127"
     CDS             9747..10991
                     /locus_tag="MIW95_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBY3152433.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKV"
BASE COUNT         4012 a         1802 c         1376 g         3914 t
ORIGIN      
        1 ataaatgaca aatctccacc tcaattaagt attccaatca tatatgaaga taatacttca
       61 ttcatttaaa aatataaaac tattatatat taaaatttct tcaataataa attatctaga
      121 ttgttacttt gatatattat ataatatcac aaaggctgat ataaaaagac aaaaagggta
      181 atttttttat atcaaaaaaa atgttaaatt gatattatcc atatataaat tgatattatc
      241 catatagatg ctatatttaa caattctact tacttccctt acacttatta gcatatagtt
      301 attaagagtt tgttgcaaaa caacacttag cacactttaa aaagtgtggt ctaataagta
      361 atcaacgaag aagacataat aaagcataaa atatgatata cttttcctgt catcaaaact
      421 gatgatttta ctattaatat ctgctaaaat atgcttaata aattaaattt tagtgggaga
      481 atagttatga aaatatttag tttcttgttg tttgtagtgg gaatatgttt ttgtattgct
      541 ggatttatgg gacattaccc agcatttgct ggtgcaacat tgagtttttt tgcaactaca
      601 atgatagctg taagacaagt caaaactaag aaaaaatgaa ataggcaaat cctaacttta
      661 caagttaata ttacataagt aattagtcta aaattggata ctttttctat tacagaaata
      721 catcatccac tttgtattct cttaaaaaaa taaaatagct atgtaaattc aagattagat
      781 agattaactc tctatccatt tcattgtaaa atatacatag ctatttgtat tcgatttaaa
      841 atacttagat tttcttacat aacaaataat ttctatctga ttattactca tattcattta
      901 gtaatcactc agcaactaat catttagtat ctattcatcc gccatacggt aacctacccc
      961 aacctcagtc aaaatatatt ctggctgagc aggattcttt tcaattttcc ttcttatact
     1021 tgccataaaa actctaagcg actgattttc actttcatag taatttcccc aaactttatc
     1081 tattataaac ttatgagtta agactcttcc tgaatactta gacatcaact caataatctt
     1141 atattcgatt ggagttaaat gtatttcttc accattaatt gacacaatat gattttcata
     1201 atctatcttt aggtttttaa cttcaaaaga ctttggagca tcattattta tattatcctg
     1261 ttgagcttta tttctaagag ccacacgtac tctagccaaa agctctgtta cactaaaagg
     1321 tttagttaaa taatcgtctg ctcctccatc taaagcttcc accttatccc tctcatgctc
     1381 tctcgctgaa acaacaatta ttttattagt ctttgcacat gccctaactc gtcctattac
     1441 ctcaatccca tcaatatctg gtagtcctaa atccaaaatt atcaaatctg ggtcaagaga
     1501 atgtataagt gatatagctg ttcctccatt atctgtctct acacacctat aattttgagt
     1561 ttcaagagat acttttataa atttacaaat tggcttatca tcttctacaa ttagaactaa
     1621 tggctttatt tccacttgtc tttcccctcc tcatcataca taggtatgct aaatctaaat
     1681 gtagctcctc cagaaggatt attaaatgcg acaatttctc caccatgagc ttctactata
     1741 gatttacata tctccaatcc aagaccaata cctcttcttt caagatttct agacttattc
     1801 ttagtataaa acctattaaa aatattaccg atatcttcat ttggtattcc ttttccatta
     1861 tctgcaactt caaatagcac ttgatttgac tcttttttta cagttagttc tatcttagaa
     1921 tttttaggtg tatgccttat agcattatca attaaattta ctaacacttg ttctattaat
     1981 aatacatcca catgaacgat tattatctca tctggaatat tggtttttat gtctctacta
     2041 ttggcaaact ttttgactct taatatagat tcagctatta tttcatcaac aacttctggt
     2101 cgtttttcaa tatccaattt cccttcatct attctagtca tactaagtat attctcaaca
     2161 gaatgagtta gccaactcgt atcttcatat atatttttca ataattctac tctagtttct
     2221 ttatctatca catcatcatt ttcaagtatt gttgaagacg aacccaatat acttgtaagt
     2281 ggagttctta aatcatgaga aattgaccta agcaaatttc ctcttaatct ctctctctct
     2341 gcctcaagat ttgctttttt atttttctca aataaatttt ctctatcaat tgccaacgca
     2401 acctgtgttg aaactgcttc taatagtatc ttttcatttt ctgtaataat atctatctta
     2461 aaacacgcaa cacctataac accaagaaca tttgattctt gaccttttat agggaaataa
     2521 tatgcactgc tgtttttgca atattttgtc ccaattccaa ctgggttacc tgtcctaaaa
     2581 gcttctttta tagcttcttt ttctaatact gaatggaata tattctcact tccatcattg
     2641 ttacaaatat aaatacttgg cttacctaga ttattattct catctgcaat agtcactatt
     2701 acagttctat ttaatatatt tactaaatta gacccacaaa attcaataat ttgattttta
     2761 ttttttgctt taagcaacgt tttgttattc tcatagagca atcttatcat tttttctctc
     2821 ttatgagaaa gtctcacttc tctttttatc ctagaagtta aagtacttgt tataattgct
     2881 gccattagca taatacaaaa agtcatagga taatcttttc tataagctaa aaaagtatat
     2941 aatggatgcg taaagaaaaa attaaatgat agcactccta ttactgaaga aagtattcca
     3001 aaaaaatatc cataggttat acttgtagca aatagtacac ctaatatgta tactagtata
     3061 atattagatt cattaaaacc taccttttca aaaactactg atattccagt agcaattgtc
     3121 ataaccaata tagtttttat gaagtcctta ataattatct ttttactgag atagccatct
     3181 tcatcataat catactcttt taagtcttca tgttgtagtc gttccttttt catttactac
     3241 aattcctcct tattgttact gtttaaattg gctctatttt atataataca attttatcat
     3301 aataaattca atcaatacat ttaattaatg tacttaaatt actaaaaggt gctgtcttaa
     3361 aatgaaaata aatctctatt tcaaaacagc acctatatat ttaaattact aatttagttt
     3421 ttacatactt aaatcaatag ttttattcaa ttttataaat tataatactt caattatgga
     3481 acatttctat ccaacaagca taaatataaa gtgggcacat atacctgcac acaaaccacc
     3541 tatggtatgc gaaagtatcg ctttaccagc caatggtctt gcatctaaag catccatcat
     3601 acctatgtgt gtacttaaat atccactcca acacataccc attgctgtga atactgctat
     3661 atcattagga gttatagcgc ctgattttat aaactctggt actaaagata ttgctgctcc
     3721 aacagctcct aatgcagtta cagggaatgc tattgcctct ggtgatgtga atccaaataa
     3781 tggttctatt atgaagctaa tcttatcacc aatagcaggt aataatttta taccttcata
     3841 tgcagctcct gtgtaaacag cttgacctgt agcagggtct gtagatggtc caaaagttaa
     3901 taacataact aatgtacaaa ctactaaaac tcctggtatt atagccatac ccatttcaac
     3961 acctagctta ccaccttcaa gtatagcatc caatgttctt tggaatacat taccatctct
     4021 tatttctcta aactcttcaa gttttgcacc tttatcattt atttgctttt cagtatcatt
     4081 tttagggtca taattataat actttttagt aaaagtcaac ataattctaa cactaataat
     4141 actacctatt attgccccaa cattacctat tatagctggt agtacatatt ctttaccttg
     4201 agctatcata aaagttgtta caataagtcc cataccaaag gctgttccta aattacataa
     4261 agcaggtact tgatacttct taaaatattg tgtaaatgtt ttgtctttag caaatggtat
     4321 aatagctgga ttatcagata aatatgttgc aactactcct gctatactag caccaggtaa
     4381 gccccatata ggtctcataa gacccttaaa tatcttatta actaaagata ttactccaaa
     4441 ctctgaaagc aacgcactaa gtgcacctgc aagaactgcc atagccatta ttaaaaatac
     4501 tgtgtctaat aaaagcgcat gtgctgtact cattattact ttaaacatca ttcctgcacc
     4561 catgatgctt cctacatatc caaatcctac cgccaatagt acaataaata cgaaggtttc
     4621 catacttact gccttgactc ttctcttatc cgacagcttt tccatcttga aatcttcccc
     4681 cttttaattt taaattaagg ttcctaacat tggtacaaat tttttggtac aaactgagtt
     4741 ctaaaactta cactcgtaat gcaagttgtc agttttttga aataaaaaaa aattacgttt
     4801 atagaataag aatatcctct ttaatttgga tactcttatt ctattttatg ttattatatt
     4861 atcatccttt tgtttaaaag tcaatttttt ctatttttgt tgtttaattt tatttatact
     4921 ctttttatat ttttcatttt cttttatctt tgtaagcact ccatataagt tgacttttct
     4981 aaatacagta gttataaata ccaatcctaa tgcaattgaa cttgcacatg ataatgtact
     5041 caccccatat tctacagctt ttatataatt attggtcaaa acagaataca tagtattata
     5101 tattccataa ccaggtacta atattattaa acaacaaaca cttaatgtag ttgctggagt
     5161 catataaatt cttgcacata actcacaata tatcgcaaaa caaactgtag ctataaaaaa
     5221 acataagttt tctgttactc ccatatttaa aagaaattta tatgaaaacc aagctatact
     5281 tccacctatt cctgctagta ttaagttttt tcctttcata ttaaatagca ctccaaagct
     5341 taaggcagta aaaaaagctg ctattacttc tataaacaaa tgcacttata tcccccctaa
     5401 cttgaagtat aatccaagta ccaagaacat tcctactgct atagatacac ctacaaaaaa
     5461 tacttctgct aattttgcaa gaccagaaat caactgtcca tctaacaaat ctcttaatgc
     5521 attagtcaaa gcaagcccgg gaactaagag cattattgct cctgctatta aatggtctaa
     5581 cgtctttata aatccgagct ttaaaattaa aattgaacaa gaagctataa taactgctcc
     5641 aattgcattt acaaaaaagt cattaagtga agatttactt aaatacataa taacaaactt
     5701 agtcagaatc ccaacagtaa atgcagctat aaattcttcc acatttcctc caaataatat
     5761 cgaaaaagtt gctgctgcaa tacctgcaaa aaatattgtt attaaatccg agtaatagtt
     5821 atcttcactt atttcatcta aaattttctc acataattca atatctaaat tatttatact
     5881 tattgctctt gataatgagt taacttcatg aaccaagttt aggtctactc ttcttgagct
     5941 tattctttta attgctgaat gtaatttccc atctacaaat acagatgcca ttacagctgt
     6001 tggagttgca aatacatcta cgtggtctat tccaaaagat tgacatattc tagctatcgt
     6061 ttcttcaact ctataggttt ctcctccact ttgcaacata gctttacctg catttgagga
     6121 aaagtttaaa attctatcta tatccacaaa acatatctcc cattctttta tagtattatt
     6181 catattttaa tacaagatat tatttattta aactatttta aataattcat atccaatatt
     6241 tacattttat gaaaaaactt gttttttatg cttgtcaaaa tcaaatttta agcttgttta
     6301 caccataaaa tggctgaatt aatatttgac aaacactttc aaccatttat ataaccttct
     6361 aaattgtata actttttact acaaataaaa ttatatctct tttaattctt tctcttgagt
     6421 atttaagctt atagcaactt catatttata tatgtacaat ataaaacatt atttttatga
     6481 tacaaaatat aatttgtaaa cgtatatatg tattaactgt gtatatataa attattctta
     6541 accttatcag ttttttcata tatcttcatc tatatcgacc atatattaat tacattgacc
     6601 atatactaat aaatgatatt acatagactg aagtatatta aaaaacttaa atttagttat
     6661 ttaacaacat aatactctaa tttaatctat aaaaatccat acctaatata atgcaactct
     6721 tattttaaca ccatctatta cacaaaacaa catattaaaa agtaaaatac aaaatatcca
     6781 ctataaacct tattttaata ctgaatatat attataatat atttaatgta gcttaaatat
     6841 agtttaaaca taaagaaagg aaatggtata tgctaagaaa agataaagtt ctattatctc
     6901 tacataaatt atgtgaaaat atgactttaa aagaccttca atcagataaa acaggattca
     6961 ctacaataga aattgctaac cattcatctc ttgatagaag taataccagt aaagaattaa
     7021 ataacctata taatgaaaaa aaagttctga aaatatctgg taagccaatt ttatttctac
     7081 atgttagtat tatagaaaat ttaattggcc gtactttgaa cagtgatgaa ttttctataa
     7141 ataattgtaa tgagctactt aaatcaagta aaaatgactc agaagatatt ttttcaacat
     7201 tacttggaca tgattctagt cttaaactac ctatagaaaa agctaaatct gctatactct
     7261 atcctccaaa aggtcttaat acgttactca taggacctac tggtgttgga aaaagtacat
     7321 ttgccgaaac aatgtacaaa tatgctgtag aaagtaaaat cttttcttct gaaagcaaat
     7381 tcgtagtatt taactgtgct gaatatgcag aaaatgctaa cttattatta agtaatttat
     7441 ttggttatgt caaaggttct tttactggtg ctaataagga taaattcggc atagttgcac
     7501 aggctgatgg aggcatatta ttcttagatg aaatacacag attaccacca gaaggtcaag
     7561 agatgctttt tcttttgatg gacaaaggca tatacagacg attaggagaa tctgacattg
     7621 tgcacaaagc taatgtcttt attgtgtgtg ccacaacaga agatatacat tcttctctgc
     7681 ttgggacttt tttaagaaga atccctgtta caatcaacct acctgcttta caggataggt
     7741 ctttaaaaga aagattatct cttattaaat acttctttat tatagaatca aaatacacca
     7801 atgcaactat aaaagttcac aaagatgtaa ttaaatcatt actcctttat gattgttttg
     7861 gtaatatagg tcagcttaaa tctgacatac aacttatgag tgcaagagcc tttttaaatt
     7921 acaaaactgg acttaaggat aatatagaaa tagatttatc ccttgttcct gattacatct
     7981 atattggtct tttaaaattg aatgaaaatc gtagtaatct aaataaatta tcagaacttg
     8041 attatatgct tttttatgaa tttaatggta ataacttaga agttgaatat acaaaagaac
     8101 tatctaaata caatatagat acatcaaaca atggatttaa atactctaca gagattcctt
     8161 atctggaaaa tgatataagg agtatgttta taaattacat ggaaaagtac tctacaaaat
     8221 tactttcgat atcttcagta actcattctg attatacaag taatgaaatt tttaaagtaa
     8281 tcaatccaaa gatttactat gctgttgaac attctttaaa agaggccgaa aaaatatcga
     8341 gaaacaaata cacaaaacaa acttatattg ctctttcaat gcatataagt gcacttatag
     8401 aaaatataaa tcaagacacc tataatccta agtacaattt atataatgat aaaattttct
     8461 tagataaaga tttaaatata tctaaaatag ttttaagtat aattgaaaat gacttacaaa
     8521 catctttacc taaagatgaa gtaaaattta tatccatgtt tctaaatagc atgtatcttg
     8581 aaccaaacaa tcaatctgaa agtataggag tcataatact tgctcatgga aattcaacag
     8641 caagcagtat gtgtgatgta gcaaattcat tgcttggtac aaaccattgt aatgcaattg
     8701 acatgcctct tgatgtaaaa gtaagtacca tacttgataa aactatagag ttggtaaaag
     8761 aatgtgacga aggtaagggt gtacttcttt taacagatat gggctctctt atagcttttg
     8821 gagaacttat ttcagaaaaa acaaaaatca aaactcgctc aattgaaatg gtatctactc
     8881 caatagtttt agaagcagta agaaaaagta cattaccaga aatgactttg gatgaattag
     8941 ctaattcctt aagctcttta aatccatata ttggacgagc agcaatcgat tcaacagaaa
     9001 taaactatcc tgaaaagtca agagagtatg taataataac tacttgtata acaggatatg
     9061 gttcagctat gaaagtagca gactttataa attcttcact taaaaatatt cgtaaataca
     9121 atattaattt agttcctcat aatatagaaa gttttaaaat gtataagaat aaatttagta
     9181 atactaaaat tctggcagtt gttggaagtg tagattttaa tgtagccaac acccctttta
     9241 tacacataga ggaattacta aatggatatg gcctagatat gctaaacaaa ataatagaag
     9301 gtacattcaa tgccaatagt attacaaata taaaagaaac aaatctatca tacaacagtt
     9361 taataataaa ctttatgacc caaaacttag aacttttgga ctctgttaaa ttattcaact
     9421 atgtcaattc ttccttattt acaataatag aattaataga tgaaaactta aagagcaaat
     9481 ttaagctagg atatatacta cattgctctt ttatgataga aagatgtttt aaaaatacaa
     9541 cttttccata taataacatt actgaattaa ttagtaaaaa tcatgaacta tatagtttaa
     9601 tacgtgaagc ctttaagcca acagaagctt attttaatat tttgatacca gacgaagaaa
     9661 ttgcttatat aatagacatg attaatgagt atctaaataa ataaaattta tagcacttat
     9721 tttgtgcata ttaattaata tatgaaatgg aggacgagat tttgaaagga aaaataaaac
     9781 agttaacaat acttgcatta atttttatat ttataactcc tgtatttgca tttgcagata
     9841 ctccaccagt accaaattct tcaagagcag cactacttat agaccaggaa actaaaagaa
     9901 tattatttga aaaaaacatt gatgaaaaaa tgccattagc gagtttaagt aaaatgatga
     9961 cctttttatt ggcaatagaa gcagttgata agaatcaagt aaaagagact gatatggtga
    10021 aaatagacaa gtctacagct tcagtaggag gttctacttg taaacttaaa gatggtgatg
    10081 aaatatctct aggagagctt atgcagggtc taatgctagt atctggaaat gatgctgcta
    10141 tagccatagc taaacatatt ggtaagactg aaaagaattt tgtaaatatg atgaacaaga
    10201 aagcagaaga aataggaatg attgatacgt attattttaa tccaaatggg cttcctatat
    10261 atactgaccc agaacataaa gaacctccta ttgaaaatat gtcaactgct cacgatatag
    10321 ttacacttgg aaagtatatg tatgaccatt atgaaaatca agttacaaga ataaccacta
    10381 tgcaagtata taatgatact aaaaaggatt ttacacatta taacacaaat cccctacttg
    10441 tttcagtacc tggtgttgat ggaataaaga caggctatac agataatgct ggatattgcc
    10501 ttgcattttc tatgatggta cctaaagatg ctaaaaacga aagaaatcat agattgatag
    10561 gtgttgtact tggtgatggt aacaagaaaa atagaatttc ttcttccgct actttattga
    10621 aatatggtaa agataatttc cattctaaaa agatagcaca taagggtgat attatagaga
    10681 ctccttgtgt tgatggtatt gatgatttta agataacagt taaggttgat aaagatttgt
    10741 atggtgttgt ttcagataac gaaaatatca atcctaaggt agtgtttaaa aatatgaatt
    10801 atcctatcca taagggagat attgttggtg ttgcaaagta ttataatgac tctggtaaat
    10861 ttgttggaag tgttgatgta aaaagtgaaa gtaatatagg atgtattcct ttgaaggata
    10921 agattaagat taaggttgct aagataaata aaaaacttga gattaaaaat tctgtttgtt
    10981 ttaaagtata gtaaagcata ctattaaaag agtatattaa atttataaat actccacttt
    11041 gtggagtttt tttgtttttt acaaaaaatt aaaatagtgg caacaattga ggatgtgagg
    11101 gaga
//
LOCUS       DAGQWR010000137        11027 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.138, whole genome shotgun sequence.
ACCESSION   DAGQWR010000137 DAGQWR010000000
VERSION     DAGQWR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.138"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(13..540)
                     /locus_tag="MIW95_003128"
                     /pseudo
     CDS             complement(13..540)
                     /locus_tag="MIW95_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            824..1390
                     /locus_tag="MIW95_003129"
     CDS             824..1390
                     /locus_tag="MIW95_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY3152434.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKND"
     gene            complement(1581..3617)
                     /locus_tag="MIW95_003130"
     CDS             complement(1581..3617)
                     /locus_tag="MIW95_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBY3152435.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLKNVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(3791..4468)
                     /locus_tag="MIW95_003131"
     CDS             complement(3791..4468)
                     /locus_tag="MIW95_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBY3152436.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSRLYFDLSTPGPNHNTKIDINPPS
                     FEKPLKK"
     gene            5163..5348
                     /locus_tag="MIW95_003132"
     CDS             5163..5348
                     /locus_tag="MIW95_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBY3152437.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(5389..7788)
                     /locus_tag="MIW95_003133"
     CDS             complement(5389..7788)
                     /locus_tag="MIW95_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBY3152438.1"
                     /translation="MIFKKYIKSLLIVVSVTLIMNKSILFVNALDLESNNNIKSYKRK
                     NVKEIDISNKFVDESFRRDIVEKVLDNRDDPLTNEVASKDNYKIYESDLNKIESCLEL
                     DLHKNPDVSNPNKIDNLDGIENFRNLEKLDCSSNNLESVDLSKNIKLKYLDISGNKIL
                     NIDLKNNNLINELHCDANKLSNLDLKNNKNLVSLFFTSNELTEIDLSNNVNLENLQFL
                     NNNLKSIDLSHNKKLKSLNCTNNKLKSLDLSNNVFLKNLSCSGNNLSKLNIKGLTSLE
                     YLGCAKNKLQDLDVSTNIKLDSLLCGKNSLTKIDISKNKELNSLECSGQYIEKSIIKN
                     GENIVVDIGIENSKLKELYPQNYNISTGFLTWENLSDFPNSISYSLEYEYGPVNDTET
                     MDVNISIDKLVKCRIRYIDYDGSIINEKEISDNSSINEIQINPQRENYEFSGWRNNKD
                     EKIYTSEQVSKLVFDDSTDFIATYKRKSTGSSGSSGGTSSKEDFNIAPSKEKITGADR
                     NETSVKISQKGWNKADNIVLINDSSISDALSATPFAKSKDAPILLTKNNNLNKLTEKE
                     INRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRISGNDRYETSIKLAKELDKNSNL
                     SKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQNDDMKDIKDLIANKNISKSYVIGG
                     ESLFNNKEVNNTLPSVTKIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLV
                     DALSVGVLAGKTESPVVLVGNGLDDSQKELIKNKKFKNITQIGGNGNEKAFNEVENLV
                     K"
     gene            complement(7882..8694)
                     /locus_tag="MIW95_003134"
     CDS             complement(7882..8694)
                     /locus_tag="MIW95_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY3152439.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            complement(8773..9636)
                     /locus_tag="MIW95_003135"
     CDS             complement(8773..9636)
                     /locus_tag="MIW95_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HBY3152440.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            complement(9897..10154)
                     /locus_tag="MIW95_003136"
     CDS             complement(9897..10154)
                     /locus_tag="MIW95_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBY3152441.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            complement(10158..10448)
                     /locus_tag="MIW95_003137"
     CDS             complement(10158..10448)
                     /locus_tag="MIW95_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152442.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNNSNRKIQDETETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKIEASTKQAHLRIDEIVNRIGGK"
     gene            complement(10479..>11027)
                     /locus_tag="MIW95_003138"
     CDS             complement(10479..>11027)
                     /locus_tag="MIW95_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152443.1"
                     /translation="VNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDRADEAFQ
                     EADSGKTLLSTAIGSPTTSEQTFQDYANYITGFKSNISNLETQLKSKYSIRYGPIDGY
                     DGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITINSNKTGFS
                     VHSYDTSYESYPFTGYYIACFA"
BASE COUNT         3607 a         1802 c         1157 g         4461 t
ORIGIN      
        1 tagctatttt ttaatcaaat tatttatttt attactttta gaacgtcttc catacttttt
       61 aatctcacga ttgattatca gatgagcaag atttagaatg attccttgct ttccatttga
      121 accacatagt ataagaccag ataaattatt actgtaatcc attatatatc tttgacttgc
      181 aaatgaaccc atgctgtgtc caaatagata aattggcaaa tcttcatttt ctttctttat
      241 aatatttgtt aaagtataca tgtcctctac aagacatcta aatccttctt tttcagctaa
      301 atgtccaaca ttttctatta tctttgcagt ttttccatga cctctatggt cattgatata
      361 tactatataa ccatttttag taagtacctt ggcaaaagtc tcataccttt gtgcagtttc
      421 agccattcca tgtgcaattt ggataactgc ctttggtttt tttatatttt catcttccca
      481 tttatatgta tatatatcta aaccttcctc tcctttgaaa gtaaagtttg tacacttcat
      541 aattatccct ccagattgat tttattcagt atattaattt atatattata ttacaagtat
      601 tttcaagtca aaatctttct aaaaaattta agaattatta attcctcata gaaattatca
      661 ttggtacata gaagttttta catacaatta tacaataatt taattaatag ttacataaaa
      721 aagtaatact ttaatatatt ttgacaccat attgacatta gaattaattc atgatattct
      781 gttttcgaca aaacaacttc taaaaataaa ggagatatat ttcatggatt attcaaatga
      841 actaaaagag ctctttttaa tgaatcaaac atatgcaact ctttttaccc ttacaaacaa
      901 aatacaaata gaaggagata aatattttgg tatacttact tcaagacagt atatgactat
      961 tttatcaatt cttcatttac cagaggaaga aacaacatta aataatattg caagaaaaat
     1021 gggtacaagt aaacaaaata taaatagact agttgctaat cttgaaaaaa atggatatgt
     1081 tgatgtaatt ccaagtcctc atgataagag agcaattaat gttaaagtta cagacttagg
     1141 aaaaaaggtt atggtaacat gtagtaggac tggaataaac tttatggcag atgtatttca
     1201 cgaatttacc aaagatgaat tagagacctt atggagttta ctaaaaaaaa tgtatcgttt
     1261 taatggtgaa gaacaagatg gctttgaaga agatgctaat tttatggaat atgaagaaat
     1321 agataaaatt aaatctgaag cacttgaaga atttgcaaaa agaagaaata gggtaaataa
     1381 aaacgattaa tactatcgca tctataatac cactttttta tagtacaata ttttatataa
     1441 ctataatgtc atttttattt tttaacaaaa caatcattac atagattagg tgttcttagg
     1501 aactcttttt ctgttttttg tacaaaaaaa ggagatgatt agacttttat tttaaagcat
     1561 ctcctttttc gctattatga ttattctact cctaaaatca tatcttttaa tttttgtaaa
     1621 tcattaattg gttgactaca actattactt tgacacacgt aataactagt cttatcatcc
     1681 ttcaatctat agttatttaa aaaacctata atagtgtttt cttcattgta tttctttgct
     1741 aaaaaagttg catttggtat aaaattttca cttatcaatt ctttaaatgc aattaaatca
     1801 gaatcttcct taaaaataca tataatttcc ttagtagaat aaagttcaaa catcaatgaa
     1861 agcatataaa agctgtagcc agttggagaa ctctttacat tatccacata cagttttaat
     1921 tgtttatatg acatttcttc aagtctatta tctccagtaa ttttcgcaag tcttataaga
     1981 ttgtataatt gaacagaatt tcctgatgga atagctccat catacaaatc ttttggtctt
     2041 gctattaaat tttcactatc tttaccatat atataaaatc cacttttttc ataatcccaa
     2101 aaaaggttga tacagctttc attcaaattt aaagcctttt ctaaatattt catattaaat
     2161 gttgactcat aaagctctat atacgcccag attaaaaaag cataatcatc taaatatgct
     2221 aaatagtcgg aacttccatc tctatatcta gctaataatc tacctgactc atttacaaga
     2281 ttattattta taaagttaag acacttattt gaatactcta aatatatgtc attttttaaa
     2341 gtactatatg cctttgttaa agcaactatc atcaatgcat tccatgatgt aagtatcttg
     2401 tcatctttat gcaaagatgt tctttctttt ctatattcaa ataccttttt actaaggtca
     2461 gctatctttt cattatgtct ttcatattct ttatttttta taagattagg aatactttta
     2521 ccttcaaaat ttccactact agttatatca aaataattat taaagaaagt tccatcttct
     2581 tcacccaaaa cttcaattat ctcaagagga ttaaatgtat aaaatttacc ttcttcacct
     2641 tcactatcag catcctgtgc tgaataaaac ccaccttctt tatccttcat ctctctaact
     2701 acataatcta tagtttttat agcaatttct ttatataact ctttctttgt tattttatat
     2761 gcatctaaaa aagctatagt aagcatagca ttatcatata gcattttttc aaaatgagga
     2821 gccaaccact ttttatcagt agagtatctt gaaaacccaa atccaatatg atcaaacaag
     2881 cctcctctat acattccatc taaagttttt tccaccattt ttaacacatc tttatctttt
     2941 tctatgctat aatatttcat taaaaacata agattctgag gacttggaaa tttaggagca
     3001 ttaccaaaac caccatactt ttcatcataa atggccttaa atactctaac tgatgaactc
     3061 aacatctctt ttgataaatc accctcagta ttttttacac caaaatcatc cttaagagct
     3121 tctattattt catctccaga ttttattaat atatccctac ttgtattcca tttttcagat
     3181 acattcttta ataaatctat aactccaggt ctattatacc ttgaatattt tgggaaatat
     3241 gttcccgcaa aaaaaggttt tttatctggt gtcattatta tggtcattgg ccatccacca
     3301 ctacctgtca tagcttggca aactgtcata tacacgctat ctacatcagg tctttcctct
     3361 ttatctactt ttattgccac aaagtttcta ttcattattt cagcaacttc ttcatcttca
     3421 aaagattctt tttccataac atgacaccaa tgacatgttg aatatcctac acttaaaaat
     3481 attggtttat cctcttcttt tgctttttta aaagcctcat cattccaact ataccagttt
     3541 ataggattat aagcatgctg taaaagatat ggagattttt catttattag attattaggt
     3601 tttctattac tgtccaaaat accacctcat ttgttatttt attatatgtt ttgcaataaa
     3661 caaatagata tacacttata ataagtgtat acccattttt aactatacat atttaatttt
     3721 tcatataatt aattcttcta gtaaataatt tttataacta ctaaaacaaa acaacataaa
     3781 aaatatcaaa ttattttttt aaaggttttt caaaagaagg aggattaata tctatcttag
     3841 tattgtgatt tggccctgga gtagacaaat cgaaatataa tctagaatct tctgtaactc
     3901 caaaatctct atttgaacct gtatctttaa ctttatttaa agcatctcta aatagagcat
     3961 tatgtgcttc ttctctgtta agtaagaaat ctattgtttc ttttacatat ttatcatcta
     4021 tttgtctata caagtactca taaactacct tagctctttg ttcagaagct atatttgaaa
     4081 gtaagtcagc tactaaatcc cctgtaactg taacataatt agcagtccaa ggagctcctg
     4141 atgagtttat taagactggt gatagaccag ttaatacatg tgtttctatt tcgcctgtat
     4201 taactgcatt ataatcaaca tcatgtccat ttaataaatt tatagtttgt gctaccattt
     4261 ccatatggct aagctcttct gctgctatat caagaaataa atcttttatt tgagggtctt
     4321 ttattctaaa actttgggac aagtattgca tagctgcttt taactctcca tttgcaccac
     4381 ccaactgttc ttgcattaaa acagcatatt gaggatttgg tctttctact tttacttctt
     4441 ttaataatgc tttatcgtgt ttaaacatat gaatacctcc gtaaattatt attcaaaatt
     4501 attatttact taagttgtaa tattattcta ggttatttgt ttattgttgt tattttttac
     4561 aagtgttggt atttcaatgt tttattaagg tcagttttgt tgtttatgtt ttattatgtt
     4621 taatttaaat ttattctatt atttattttc ggtggcaaaa tggtggcaag tttggttttg
     4681 tcaccattta tttttatttg taaaaaatat agtttatcaa tataacttta ttgaaaaata
     4741 tgtaatattt attgactttc acgtcacgtg atgatataat ataaatatag aaaggaggta
     4801 agaaagtggg gagattagaa agaagaaaaa ataaaagaga aaacaaattt aataaaatta
     4861 aaaatgcttt ctctttcaca ttagttttaa ttaatctagt actcgcaata ctaagattga
     4921 ttaaagaatt ataagtttca atcctaggag gaaaatacct tccttctagg tagcttcttt
     4981 ctaatattat aacacatttt ctaatttaat atgaataata aattttatag aatttgtatg
     5041 attattcttg gtatcactat agttttaaat attattaata taataataag ttttactttt
     5101 aaagctctta ttgctttggt atttacttta ttattgctgg catttattaa tagtagaaag
     5161 gaatgatatt attggagcaa agaaaactta gaattggatt taataaaagt ggcaaaggct
     5221 cttttacacc cagattgata cttccaatgt catggattaa agagatgaat atctctcctg
     5281 atgaaaggga tgttcttgtt acttttgagg atggaaaaat aattattgaa aaaactgaaa
     5341 atgaataaaa aaggaggtag taactatttc cagttactac ctttcaatct atttaactaa
     5401 gttttctact tcattaaatg ctttttcatt tccatttcca ccaatttgag ttatattttt
     5461 aaacttttta ttcttgatta actctttttg gctatcatct aatccatttc caactaatac
     5521 tactggagat tcagttttac ctgcaagcac tcctactgat aaggcatcta ctaaatcgtc
     5581 ttgtttattc attccattct tagctacata taagtcatta agtgtatctt tgctatagaa
     5641 atgacttatt acttttgagt tagtttctgt tctatcagaa cctgctattt tagttacaga
     5701 tggtaatgta ttattcactt ctttattatt aaataaagat tctccaccta ttacatatga
     5761 ttttgatatg tttttattag ctattaagtc ttttatatct ttcatatcat cattttgatt
     5821 agtaagaaga attggcattt cctcttttgc tgatatagct cccatactta ctgcatcagc
     5881 tagtcccttt tctccattta ccactactac ttttgacagg ttagaatttt tgtctaattc
     5941 ttttgcaagt tttattgatg tttcatatct atcatttcca gatattctaa ttacatttaa
     6001 tccttttttc tttaaatcag acactacttt ttcatcaaca gactttaatc cacccactat
     6061 gtacacattt tttgcttcta atctatttat ttctttttct gttaatttat ttaaattatt
     6121 atttttagta agtaatattg gtgcatcttt agacttagca aatggtgttg ctgataaagc
     6181 atccgatatg ctagagtcat ttattaacac tatattatca gctttattcc atcctttttg
     6241 acttattttc actgatgttt catttctgtc tgcacctgtt attttttcct tagatggtgc
     6301 tatattaaaa tcttctttac ttgaagttcc accagaagaa ccacttgatc ctgttgattt
     6361 tcttttataa gttgctataa aatcagtact atcatcaaaa actaatttac ttacctgctc
     6421 tgatgtatat attttttcat ctttattgtt cctccaacca ctaaactcat agttttctct
     6481 ttgaggatta atttgaattt catttatact agaattatct gatatttctt tttcattaat
     6541 tatacttcca tcataatcta tatatctgat tctacatttc actaatttat ctattgatat
     6601 atttacatcc atagtctctg tatcatttac tggaccatat tcatattcca aactataact
     6661 tatgctattt ggaaagtcag aaagattttc ccatgttaga aaacctgttg aaatattata
     6721 attttgtgga tataattcct ttaatttcga attttcaatt cctatatcta caactatatt
     6781 ttcaccattt tttataatgc ttttttctat atattgacca ctacattcca gtgaatttaa
     6841 ttctttattt ttacttatat ctatttttgt taaactattt ttcccacaaa gtaaagaatc
     6901 taattttata tttgtagata catctaaatc ttgtaattta ttcttagcac atcccaaata
     6961 ctcaagactt gtaagtcctt ttatatttaa tttactcagg ttattacctg aacaacttaa
     7021 atttttcaaa aaaacattat tacttaaatc aagggattta agtttattgt tagtacaatt
     7081 tagagatttc aactttttat tatgacttaa atctatactt tttaaattat tattaagaaa
     7141 ttgtaaattt tctaagttca cattattact taaatctatc tctgttaatt cattactagt
     7201 aaaaaataaa cttaccaaat ttttattatt tttaaggtct aaatttgata atttatttgc
     7261 atcacaatgt aattcattaa taagattatt gttttttaaa tcaatgttta atattttatt
     7321 tccactaata tctagatatt ttaattttat atttttactt aaatctacac tttctagatt
     7381 atttgaacta caatcaagct tttctagatt tctaaaattt tctatcccat ctaaattatc
     7441 tattttgttt ggattagaaa catctggatt tttatgtaaa tctaactcta agcaactctc
     7501 aattttattt aaatcacttt catatatttt atagttatct tttgatgcaa cttcatttgt
     7561 taatggatca tccctattat ctaatacctt ttcaacaata tctcttctaa aggattcatc
     7621 aacaaattta ttgcttatat caatttcctt tacattcttt cttttataag attttatgtt
     7681 attgttactc tctaaatcta atgcatttac aaataatatt gatttgttca ttatcaatgt
     7741 aacactaaca actatcagta atgattttat atacttctta aatatcataa tctcccccca
     7801 tacctattaa taataaataa tatttatcta aatattacca tacttatgat tttcaagaaa
     7861 tactaaaata ttttatataa tctatctatt aataaaatct aatgccttat aaagtgtatc
     7921 aaatctatca ttacccttta tcatagtaaa tttttcttta gtcatagaac caatcttctc
     7981 acatgctgca ccaccaatga cataaagatt ttgcgtctga cctggcacgt aatcttttat
     8041 atcacatatc agtattttcc catcattata accccaacca actacagttg cagagatttt
     8101 gtcaacttct ccatcataaa caattgtatg tttgtacatc tgtttaactc cctcattatt
     8161 tatattttta tttaatacac cttctacaat taacttagca ataccttcat gacctagttt
     8221 cttagctttc tcataatctt ctttattatc acagaagaaa ctttcaatta atactgctgt
     8281 aggatttgaa ctatttaaga tatataatcc tttatctaat ttagctcttc tatttctaaa
     8341 tactgtacct agcttattac atattctagt tgcatactct aaacctttat tactataata
     8401 tagaacttct gaaccttttc cttgaccatc acttgcattt agatgtagtt ctataagtaa
     8461 atcatatcct ccactattaa ctctaggtat tttataagtc ttttcttctg ctttagtttt
     8521 aaactgcttt tcaggacata ttattacatc tgccttatga ccctcttttc taaatgtatc
     8581 tgctaatact ggtgcaagag atttattgta ttggtattcg ttaactactc catcagcaga
     8641 agtgcatgca ccacttttta aaatactgtg tcctactgta atacatattt tcattattta
     8701 ttttcctcct tcttttcaat aaaaaaacac ttacatatag taagtgttta taaacttctt
     8761 aaagtaacta ttttatttag ccaatatctc tatccaatta ttaggaaaac caataagctt
     8821 taagtctata gaattattat aatcattaat taacttttga agttttataa aaaaactatt
     8881 ccaaattaat ttatccatag tcaaatgctt catagcaagt atataagaaa atatcttttt
     8941 attatccaag ttatactttt tatattcgtt ttttattgtt ataatacgga aattattatt
     9001 atatattcta ccataatgag cgcattgatt tctgacctgt gttaatgatt gtaaccaaga
     9061 tttaactaat aaagtattca ctctacacaa attatttttt atgtatctag tatcttctgg
     9121 caacatgttt gaatataatt ttgacaacat accaaaagtc attatttccg ttgcaaccca
     9181 aatgggaagt tttccttcat atttttcttt atggtgtata ataaaaagct tatctgaatt
     9241 attacttttt tctctctcta atgctgtcaa aaaattaata tagaattttt catctttgaa
     9301 actctccctt tctagatatc cacaagcacc atgtttaatt gctaatgtat atgcaatgta
     9361 tgtcctaaat gctatttcta tgctccctaa caaatctgtt aataatatcc taaattcctt
     9421 attaaaccta tatatatcat aaacttcttc aaacgtagtt ccttctttat atgagccatc
     9481 atcattctta aaacttagaa gatatgctgt aaacctataa taattgacat tacttaatac
     9541 aaatttagca tcttctttat catttattat taatcctcta ctttttaaaa tatctatttg
     9601 ttcatcaaat gttttttctt cttttacttc aaccattgtt ttcacctttt catagatata
     9661 cttttatata ataaaaccca ccaattaata ttagtgggta aatttatctg tcccgcctat
     9721 ttgagctata aaagaagctt ggcgggttcc ttcaaaaatc aatgtcccgt atatttgagc
     9781 atataaatgc tttacgagtt ctgtactatt attatatact tattagctat aaaagtatac
     9841 tctatttaat gaaatttttt aaatttttgt gagtaataat atttttgtta atttcattac
     9901 ttttcaccat ccttcaactg tttgtaagtt tgatttatac ctattgcaac tccccaacaa
     9961 attacacctt gtaagactgc aacaggattt aatcctagca tccatattga gaaacctatt
    10021 ccaagtatca gtaacactac tggaatgtat ttattatcta attgtttata ttttttacaa
    10081 cctttaccta taatagagag agcagctact aaaattagca actgctctgg tataaaactt
    10141 attaaattat ccatctctta ttttcctcca attctattta caatttcatc aattctaaga
    10201 tgtgcttgtt ttgtacttgc ttctattttt gcaactcgtt cactaatatc taagaaacta
    10261 gtattaaact ttgctacatc atgctttata tctcttacat tctcacataa aaaagttatt
    10321 tgttgttcta attttgtagt tgtttctgtt tcatcttgta tctttctgtt tgagttattt
    10381 ctaaatgcta agtaggatat tacaactcct gctactgcac atagtagatt tatgctaatt
    10441 tcttccatac atcctcctag ttttaataat ataatttatt atgcgaaaca agcaatataa
    10501 taacctgtaa aaggatatga ctcataacta gtatcatatg aatgaactga aaaaccagtt
    10561 ttattgctgt ttatagtaat ccatgcacgt tcagaacctc ctgtattact tcctaaagaa
    10621 ctaccactgg gattataata atatacactt cttctaaagt aaacatagac aataaggtaa
    10681 cttgcactct tgccaaaatt agcagaaaaa ggattaccat catatccatc aataggacca
    10741 tatctaatag aatatttgct tttcaattga gtttctaaat tgcttatatt gctcttaaat
    10801 cctgtaatat agttcgcata atcttgaaat gtttgttctg atgtagtagg agagccgata
    10861 gcagttgata aaagtgtttt tccactatcg gcttcttgaa aagcctcatc tgctctatct
    10921 atacattctt tcaatgctcc ttctacattt tcacttgtaa atttactttc tgtatcttct
    10981 atagttacat tctttgcttc taatacaaga tttctaactt tattaac
//
LOCUS       DAGQWR010000138        11016 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.139, whole genome shotgun sequence.
ACCESSION   DAGQWR010000138 DAGQWR010000000
VERSION     DAGQWR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.139"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(32..883)
                     /gene="gatY"
                     /locus_tag="MIW95_003139"
     CDS             complement(32..883)
                     /gene="gatY"
                     /locus_tag="MIW95_003139"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBY3152444.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(932..2101)
                     /locus_tag="MIW95_003140"
     CDS             complement(932..2101)
                     /locus_tag="MIW95_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBY3152445.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     NRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPQGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2563..3480
                     /locus_tag="MIW95_003141"
     CDS             2563..3480
                     /locus_tag="MIW95_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBY3152446.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     ICSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVNQ
                     GEELIELSKKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKHFSVNTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(3609..4538)
                     /gene="pfkB"
                     /locus_tag="MIW95_003142"
     CDS             complement(3609..4538)
                     /gene="pfkB"
                     /locus_tag="MIW95_003142"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBY3152447.1"
                     /translation="MITVITFNPSIDRMYKVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEYNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            4807..5532
                     /locus_tag="MIW95_003143"
     CDS             4807..5532
                     /locus_tag="MIW95_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY3152448.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRIQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            5615..6769
                     /gene="nagA"
                     /locus_tag="MIW95_003144"
     CDS             5615..6769
                     /gene="nagA"
                     /locus_tag="MIW95_003144"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBY3152449.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQRKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFSVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYVPALSCNIDNVCGSIHQGMDADFIVTDKNFNIYETYISGICMYKVNK"
     gene            complement(6953..8110)
                     /locus_tag="MIW95_003145"
     CDS             complement(6953..8110)
                     /locus_tag="MIW95_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBY3152450.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            8264..8833
                     /locus_tag="MIW95_003146"
     CDS             8264..8833
                     /locus_tag="MIW95_003146"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBY3152451.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            complement(9015..9431)
                     /locus_tag="MIW95_003147"
     CDS             complement(9015..9431)
                     /locus_tag="MIW95_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152452.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(9657..10751)
                     /locus_tag="MIW95_003148"
     CDS             complement(9657..10751)
                     /locus_tag="MIW95_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152453.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
BASE COUNT         3820 a         1779 c         1345 g         4072 t
ORIGIN      
        1 ttatctaaaa taaatttagg atattttatt tttatactct atttacagac ccacacactt
       61 taatcttttc ttttacaaaa tccttcactg attgaagccc tgctaatcca tactttttag
      121 ggtctataac ctcagaatct tcatctaaaa cttttttaac ccctttacta tatgcaattc
      181 ttaattcagt tgcatagtta acttttgcaa tacctctttt tatagcttct tttataatat
      241 catcatgtat tcctgaacct ccatgcaata ctaaaggtat tgaaactaca tttttaattt
      301 ctgataacct atctaaatct attttaggtt cacccttata caatccatgt gcagtaccta
      361 tagcaatagc caatgaagat attcctgttc tatcgacaaa ctcttttgcc tcttttggat
      421 ttgtataatc attactattc ccgccatcta aatcatcttc ttttccacca actttcccaa
      481 gttctgcttc cactggtata agagaaggac tacatgcttt tactactgat ttagatatag
      541 atatattatt ttcaaagctt tcatgagaac catctatcat tattgatgta tatccacttc
      601 ttaaagcttt cattgcaagg tcaaaacttg aaccatggtc cagatgcatt gctacaggta
      661 tctttactct ttctgcagca acctttgcat ttgctagata ataatcaaga cctgcatatt
      721 ttacagtaga tggagtagtt tgtaatatta ctggagagtt taactcctca gctgcttcta
      781 taacagcaat taccatttcc atattttcta cattaaatgc tcctactgca tacccctcat
      841 cttgtgcctt tttaagcatt gatgttgttg ttactaatgc cacattgata ccctcccaaa
      901 ttatttagtt ataatatttt aatcttatct tttaatattc atatattgtt acacctttta
      961 ctactctatt tactgttcct gtagctgatg gcgtatctgg cttatttcca acctttaccg
     1021 atacaaacaa tgctatagtt tgtgcaaaca atatgtatgg cattgcaaga tacacatctg
     1081 gtaatttatt atacttttct ttaaactcaa actttgttcc tgagaagtta ttttcatttt
     1141 ctactgacac tgcacaagtg aatttagcaa ttttatccct ttttatctcc tctaaaacat
     1201 ctaaatcata ttttcttgta tataaacagt tagatacaaa ttcaaataca agtgtatttt
     1261 catcaataaa tgattttggt ccgtgtctaa accccatagg agaatcatac accgtagata
     1321 tttttcctgc tgtaagttct aaaagtttta gttgtgcttc ttgtgtcaaa ccacctaatc
     1381 ctcctgaacc taaatataca actctatcga aatctttatt tattaactca tgtatttcat
     1441 cttttctatc tataacattt cttcccattt caattattgc atttatatat gacttttcta
     1501 catcatcttc taaactatcg aatattaaca tagcagataa catcatacac gaaaaacttc
     1561 ctgtcatagc aaagccttca tcatttgatt ttgatggcat taataacaag taattatttt
     1621 cgtcgttttt agtcatttta gctagttctc cttgcggatt acaagttata gctaaatgat
     1681 aaaaattatc tacaatctta ttccctaaat ttaaagcagc caaactttca ggactatttc
     1741 cacttcttgc aaatgaaatt aataatgttg gtatatcttt ttttaagtaa tcatatgggt
     1801 ttgaaactat atccgtagta ggtattgctt caaatatgta ttttcttatg tcatttttat
     1861 tcttaaggta aggaagtatt gaatttccta tgtaagctga agttccagca cctgtaaata
     1921 taactctaaa ctgcccatga ttattactta ttgtatctat gaatctattt aatttttctt
     1981 tgttagattt atatatttca taagtctcta accataactc aggttgttgt tttatttcat
     2041 tagttgttat tattgcacct aaatctttta gtttttgttc ttctaattta aacattttca
     2101 ttctcctttc atacttgtaa cgtttacatt tggtaatgag gttataacca ctttaaacta
     2161 taaaaaaata tactctttta taattttttg cttagatttt ttagtactac ttaaactcac
     2221 tataaaatat acgctataga ttttaattaa ttttatagtt tatctttaaa aatttaataa
     2281 aatcatactt tagtattttt attttacgat aaaaacttgt tcagaccaaa gtggtaatta
     2341 ccactaatcc aaatataaat taatttgaat tttttgtcaa tacataaaat atttatatca
     2401 atcaatttta tagatatcct acaaaattac tttgtcaata tttcttattt cgttaattta
     2461 cttatataag ctttaaattt attttttgat acaaagttta caaatttatg tgtctgaatt
     2521 ataatcaatt ctgtaaatat aaaaacctta ggagaaagta ttatgaaaaa tataaaaatg
     2581 ggaatgatag gtcttggctc catagctcaa aaggcatatt tacctatatt aactaagtct
     2641 gagagatttg agtttgtagg agcttttact ccaaataaag ttaaaaggga aaaaatctgt
     2701 agtgattata gaattatgcc attcgattct attgaatccc tagctaaaaa atgtgattgt
     2761 atttttctac atagctctac agaaacacac tacgaaatta taaaaatatt attaaattta
     2821 ggtgttcatg tatatgtaga caaacctctt gcttcaacag taaatcaagg agaagaactt
     2881 atcgagttaa gtaagaaaaa aaacttaaat ttaatggttg gattcaaccg tagattctgt
     2941 cctatgtaca aagaaattaa aaataatgct acagaaatcg tatctatcaa tatatgtaaa
     3001 catggattaa attccttaag aaatgttaga ttcgacagta cccttataga tgactatata
     3061 catgttattg acactgcttt atggttagca aatgaagatg ttgaaattag cggagaagat
     3121 ttatttttaa cagacaataa gaatcttata tttgtaagtc ataaacttaa aggtaaacat
     3181 ttctctgtaa acacctctat gcatagagat tctggaacaa agttagaaca agttgaaatt
     3241 ctttcaaaag gtaagattca aagagttaag aatcttaatg tactggaaat agaagaaggt
     3301 ggaaacctaa ctttaaaaca aagtggtgca tgggtaaata tattaaaaca aaagggtttt
     3361 gaggatatat caaatcattt tattgattgt atagaaaata atataaaacc agctattaat
     3421 ggagaagagt gtatcaaggc tcaaagatta ctagagaaaa ttataaattc agtaaaatag
     3481 atatttatta taaaaatagg ttaaaaatga atttttctaa cctattttta tataagacta
     3541 tatcttttat attaaaatta aaatctatta caacaacatt aaatttatta tattcccatt
     3601 aaatttattt aacttaactt ttttacttca actaaatctt gatatttatc aatttctccc
     3661 aaatctacaa atccagtttc catatttaaa gcattactta taccgcaagc atttgcaaat
     3721 ttaagtaaat tttctatttt ataacccctt aaaataccta cactaaatcc agccactgtg
     3781 ctatctccac tacctacagt atttatacac tttacaactg gtacttttac atcatataca
     3841 ttatattcgt ttaaatacac cataccatct tttcctaaag atatacatac attctctgca
     3901 cccatttcaa taagtttttt tcctgacaag ataatttcct ctcttgattc tatatttatt
     3961 cctaataaat gtcttatttc atctatattt ggctttatta aatatggttt tgattttaaa
     4021 gctattttta atgcttctcc tgatgtatct aatataaatt ttatattatt tgctctacat
     4081 atatttccta tcttttcata ataatctatc ggcatattct cacaataact accagaagct
     4141 actagtattt tagtattttt tagtacttcc tctatatcat tttcaaatct ctctaaatcg
     4201 cttttttcta ctattggtcc tttttctaaa aattctgtac taactttatt gtcctcaatt
     4261 atgtttaagc aatttctagt ttcctgctct atttttgtaa atttattttg tatatctagt
     4321 tttctaagtt cattttttat aaattctcca ttaaatccac ctagaaatcc catagctaaa
     4381 ggattttctt ttaaaatttt acatacctta gctacgttta atcctttacc tccagcagtt
     4441 gcattcttac ttactaccct ttgcacctca cctatgttta tattattcac tttatacatt
     4501 ctatctatag atgggttgaa tgttattact gtaatcatac tagattctcc tcattattaa
     4561 tttttacttt tctaaacaat aaattaatct gtaaaataaa aaatacctca tctataatat
     4621 acccaaagtg gttattacca atttgaatat aaattatttt acttgttttg tcaatatacc
     4681 ataattttat aataaattaa tagccttact tattgaattt tgattaaact ttattcattg
     4741 acataaataa ttctatattc tataatcaaa ttgtggtaat aaccacttag aaaggactat
     4801 gtttttatga taaaaagaaa tgataaaaga ccaatatatg accagcttgt tgagatatta
     4861 agaactaaga ttgaaaatga gatggaacca aacgatagaa tgctttctga aagaaaaatt
     4921 tgtgatgaat atggagtaag tagaaccaca gttagactag ctatggctga gttggaacat
     4981 atgggatata tatacaaaag acatggaaaa ggaacttttg ttgctgcact aagtcaaaat
     5041 tctcaaaact taatggaaag ttatagcttt acagattata tgagaataca aggaaaaaag
     5101 ccaggtacaa aggtattatc atttgaggta gtagaaagta caaagtattt ctctgaaaaa
     5161 ttaggtataa ctcctggtga aaaaatgata aaaataatgc gtttaagact tgctgatgac
     5221 cttcctatga tgcttgaaag aacatatctt cctatgaaag aatttatagg attgacaaaa
     5281 gaaaaaatcg aacaaaaacc tttatatgaa atatttagag aagattatgg cgagattatt
     5341 aaagttgctg atgaagaatt ttcagctgga ataatctctg ataaggaagc taagttattg
     5401 gaagtaccag gagattcacc ttgtctgaaa ttacttcgta ccacatataa tgatgacaat
     5461 atagttattg aatttacttt gagtgttgct agaagcgata aatttgtata tagagtaaga
     5521 catgtaagat agtgaattaa atagctttca agaaaataga atgatattat aaacgtcaat
     5581 gccacaatga tagtataatt ataaggaggt tattttgaaa agttttattt atgcagataa
     5641 attttttatg aaacatggaa tcaaagaaaa aggttacctt agtataatta atggcaaatt
     5701 cggtgaattt caacaagaaa gacctaatat tactgataat gtcatagatt tttcaggtaa
     5761 atatattgct cctggcttag ttgatactca cattcacggt cttttaggtg cagatgtcat
     5821 ggacaataca tttgaagcaa taaacacaat ttcaaaaggc ttattaaaat atggagtaac
     5881 atctttccta cctacaactt taactgattc tgtagataca ctaagtgatt ctgtagaaaa
     5941 tataagcaat agcttcaatc gagttgaggg tgcaaaaata caagggattt atttagaagg
     6001 tccatttttt acagaaaaat ataagggcgc tcaaaatgaa aaatatttta aagaccctga
     6061 catcaatata ttaaaattat ggcaaagaaa atcgaaaaat ttaataagaa aaatagcaat
     6121 tgctccagaa cgtaaaggtt caatagaatt aacaagatat gcaactaaaa ataatatctc
     6181 tgtttctctt ggccatagtt cagctacatt tgaacaagtt aaacaagtcg ttaattcagg
     6241 tgctaaagtg tttgtacata catataatgg gatgagccct ttaaatcata gagaacctgg
     6301 aatggttggg gctgctatgg tactaaaaga tacttatgct gaattaattt gtgatggtca
     6361 tcatgtaagc ccaatagctg ttaaaataat gatggatgcg aagtctagta ataacatagc
     6421 tttaattaca gattgtatga gggctggtgc tatggaggat ggaaaatata ctcttggtaa
     6481 gtttagtgta aatgtaaaaa atagtgttgc aagattaaat agtgggagtt tagctggaag
     6541 tgtattaact atggacaagg ctgtgagaaa tatagttgat tggaacatag ccactttaga
     6601 agatgcaata aaaatggcta catatgttcc tgctttaagt tgtaatattg ataatgtctg
     6661 tggctccata catcaaggta tggatgcaga ctttatagta acagataaga actttaatat
     6721 atacgaaact tatataagtg gtatatgtat gtacaaagtt aataaataga ttcaatataa
     6781 agttatggag gcttcctaaa aatagagata agtctctttt tcattgttaa aatgatgaaa
     6841 atacatttga tttcatacaa aaagagtgcc tcataaacta aaaagttaat tttgagacac
     6901 tccttttatt aaggttcttt attttacaat ataagtttat aaaaattaaa ctttatttat
     6961 caatctgttc tctaagccat ttagtcgcta catctaactg tttattttca tctttttctt
     7021 caatagtagg ctttatgcct ttttcattga tataatctcc attaggtgta aaatattgag
     7081 cagtagttaa cttgtatcct gttccatcct tcaatcttac aactgattgt accaaccctt
     7141 taccaaaagt tgtagtccct acagaaatac cttctttatt atcaacgatt gcagctgtta
     7201 atatttcagc agcagaagca ctttctccat tagttaacac tactattgga agtcccaatt
     7261 tttctttatc agatttcaag tactcagtat ttcccttatt atcttttgta tatactatag
     7321 ttccttcacc aattaactca tcagcaactt ctttacatat atctagcatt ccaccaggat
     7381 tatttctaag gtctattact aatcccttga tattttttgc ttttaattct tttaaagctt
     7441 tatcaaagtc agtatatgta cttgatataa attgggtaat ttctatatat cctaaatcat
     7501 tatcaattac tttactttct atactcttct caattatctg gtctgttttt actttaaagt
     7561 tcaattgttt atcttctctt aatatagtca gttcaactgt tttccctttt ttacccttca
     7621 tcatacgaac agcttcatct gaatttttat atgagacaga ttttccattg acttttacta
     7681 atttatcccc tgattttaca cctgctttct ctgctggaga atctttcatg ggaactacta
     7741 ctaagccacc atcttttgat gcccctatat acatacctac accaacataa gaaccttcac
     7801 tatcattaat tagattctcc atttcatctt tagtataata accagaatac ttatcatttg
     7861 ttcctagaaa tagacctttt gctgctccat ttaccaaatc agtatctgat acttttttat
     7921 aaaaatcttg ttttataata gattctagtc ctaacaactt atcatatttt ttatagtctt
     7981 catagagttc tttcgatatt acaactttat tccccaatgt caactgaaat gctgatgtaa
     8041 ccatagctgt tataattact aaaatcaccc ctaaaaaaat agctttcttc tttgaaatca
     8101 caagatacat ctccttttac ataaactatt attattatat gactagctgt tatttttatt
     8161 acatttaccc aaatatattg ctttattaca ataaattata ctaaaattta tattaaatac
     8221 aattgctgta cacattataa catattaagg gaggaattat tttatgaaaa aagaatttag
     8281 aaaaaaagtt attgaatcaa gaaaaaataa gagtgcaaac tttgtactta gtaattcaaa
     8341 tttaataact gaaaaattac ttcaaatgga agatataaga aatgcaacta atataatgct
     8401 ttatctaaat tttaacaatg aagttcaaac agataattta atcaaaaaac ttttgtctct
     8461 aaagaaaata gtatcaagtc ccatcacaat aaaagaaaat catacattaa tccctactca
     8521 aataacagat ttaaaagagg gattgagaat tggagcatat ggaataagag aacctaacga
     8581 aaaatcacct acaattgata ttaaggcctt agatgttgta attgtaccag ctgtagcata
     8641 tgatatacat tgctatagat taggatatgg tggaggattt tatgatagat ttttagaaaa
     8701 cttaagaaaa gatgctataa ctattggcat agcatttgac ttccagatat tcgattctat
     8761 tccaaaagaa actcatgatg cacaattaga ctacatagtt actgaaacta gaattatcac
     8821 tccaaataat tagaacatta agtatttcca gaccccattt aatatagtaa taaatactac
     8881 tgttaatatc ttactttata agcattttaa gtcatggctt ttacaacaat aagaaccata
     8941 tacaatttac ttacattaat aaaaaccatg actttattgt acaatatgca gttatttaat
     9001 tataaaatac cttcttattt cgtcgcattt aattctattc tatctggaag ttcactcata
     9061 tttgtttcaa caactatgta tggaaaagaa tcttccttat ctttatactt tatcttgact
     9121 ctcaaaacat cctttccttc ctcaaccaca ctttctattt tgtccatttc tataccatat
     9181 ttattatttc ctctagtcac tataatgtat attttgttat cgattttagc cgctaatgct
     9241 ctttcttcat ttgtatactt atccatcaca tctaatatct tttctggtat tgaatttctt
     9301 tgtaatactg tataatctat gggtttagat ttctctacaa acatacttgg tatgaatata
     9361 aatcccccta taacgaatac aagagctata actccataaa ttataccttt aatattaaat
     9421 tttacattca atcttatcac tcctcccata tattctaaat atatgattta taagcttatc
     9481 ttagactcta aattattgat tagtatatat ttatccataa ttataaatat atgtaatgta
     9541 actaaaaaac atgtttttcc tatttcataa taaacgagct aaaaaataaa aagagctata
     9601 tgaaaacatt ttcattgttt tcatatagct cttgagttta caatattaaa aaatacttat
     9661 ttgtcactga ttccttcttg atatccagat gtttttgtaa tctgtctata aagaacacaa
     9721 accagtatta tgacgcccat atatccagta tatggatata aaataccaac cagcttatca
     9781 aatggtagga agccacctat aagacctaat atagctgcta ttaaagttat taatttaaac
     9841 ttaggatgtt tatcatctac aaatctatta gttactgacc ataataaagg tacagctgtt
     9901 gtatatatac ctaatataag aattattgag aataaaactc caattactgg tgaaatctta
     9961 tctgctaaga ataatgatgg tattgctttt gaatataagt ttcctatatc agaaagaata
    10021 gctaagtgca tcattatagc tgcagccata agtgctactc cacctaatat accaccacaa
    10081 atagcttctt ttttgttagc agcacttgct cctaatccag ttaagaatgc cataacaaca
    10141 attgtgttat aagtagtata tattacacca gatagatagg catttggaac tggtttagct
    10201 atctttacag tgctaagaac ttgtgcagct tctcctatat tacctatgtt tttagctaaa
    10261 cttataccac caactaaaag tgtaaagatt attattatag gccctattcc acctagtatc
    10321 tttgataatt tatcaagacc tagtgaaaca gttaataatg ctactactgc catacctact
    10381 ctacctacaa atggatttaa tccatagtat tcagttaaag ttgctcctgc tcctgaaatc
    10441 attactacga aaacaccaaa taagaataaa ggtccaaacc attcaaagaa tatccctaaa
    10501 tacttaccac aataaacacg ataaattttg ataggttctt taagttgaag ctctttacct
    10561 tttgacataa ctgaagcacc catccatgag aataatacca ttgaaattat cccaccaata
    10621 atacctgcaa aaccataaaa agagaagaat tgcattattt cctgtcctgt tgcaaatcca
    10681 gaaccgatta ctgttgctac atacgcacct gcgaaactga tgactgtttt aacatttact
    10741 ttttctgaca tatctcagtt acctccccat gtgaaatttt ttcttctgat taaataattc
    10801 gataaactac ctacaatttc ctttaacata aaatacatga taactgtttt atagcttttt
    10861 agtataataa cttaaaactt ataatctgat tttataccct actcctcctc tcataatttt
    10921 tttagattct tttatctaag tagtcattat atataaaatt agtttctcta tagattatat
    10981 attagtcata tttttctgaa tagtcaatat atattt
//
LOCUS       DAGQWR010000139        10889 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.140, whole genome shotgun sequence.
ACCESSION   DAGQWR010000139 DAGQWR010000000
VERSION     DAGQWR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.140"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            457..1317
                     /locus_tag="MIW95_003149"
     CDS             457..1317
                     /locus_tag="MIW95_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY3152454.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1307..3178
                     /locus_tag="MIW95_003150"
     CDS             1307..3178
                     /locus_tag="MIW95_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY3152455.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRLTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEASNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3198..4652
                     /locus_tag="MIW95_003151"
     CDS             3198..4652
                     /locus_tag="MIW95_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBY3152456.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLMDLYLKYSETVI
                     RRFDDKVKYWITFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            5676..6884
                     /locus_tag="MIW95_003152"
     CDS             5676..6884
                     /locus_tag="MIW95_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152457.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNVLPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFRLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            6915..8294
                     /locus_tag="MIW95_003153"
     CDS             6915..8294
                     /locus_tag="MIW95_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY3152458.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELSAY
                     GHGCGHNLLCTAHVGAVVGLKREMLENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            8400..9590
                     /locus_tag="MIW95_003154"
     CDS             8400..9590
                     /locus_tag="MIW95_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY3152459.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEESTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEIIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(9718..10782)
                     /locus_tag="MIW95_003155"
     CDS             complement(9718..10782)
                     /locus_tag="MIW95_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152460.1"
                     /translation="MFLKLIFKYNTLDFSRAYFDLTDWADNPKDISYYLSNGTFGDLM
                     DSIDENASKMSLSDIAKLEKKIAKKHINLWNEGKIDFSTTRFSSIVPMLQNAGVNFHF
                     IYPDIDLLKNTFNNLIKDIHLHRMKGNQPSVIYITVKDTNYDDSTLDQVYKILNSIKK
                     DKLTDFIIEKNSNSFKIFTKCETIKSLTKNFTACYIKESIENSTNIKVCVGYGIGNDI
                     KQAMSSAISANKESKINPLNYSFLVNENYETIGPLLCDKLLTVSNKVTPHINTIAKSS
                     KLSTLTIQKIISVTEILQRDEVTPKELSSCLNVTVRTANRFLSSLLKSGNATILYEKQ
                     NSTKGRPERVYKLFISENKY"
BASE COUNT         4058 a         1224 c         1968 g         3639 t
ORIGIN      
        1 ataaaactaa atttgaagct ttacctatta atatatctta atagtgatta aataaaacta
       61 aacttaagaa tttatttatt aacatacagt tcattatatt taaaatcatt actatgataa
      121 ttcataatag taaaatagat aatggttcag acaatattgt aaatattagt gacagtaaaa
      181 tagtatcaat ttagtatata aactttgcca gataatcgca taacagagct tgtagttata
      241 gaataaaaaa atatctagga aaccgttgac agatttatat ggatagtata acatatagaa
      301 tatgaaatta aacaaaaaag aattttaatt atggcgacgt agtcataatt ttgagtatgt
      361 tactgattag caggcagaac tcaagtatat atgtgaaaaa tagagggata ctctatacat
      421 atatgcttga gttcttttta ttgaaaggag gaatatatga aaataattca gattttaaat
      481 aataatgtag ccttagtaaa aagaggtaaa aatgaaatta ttgtatttgt taaaggaatt
      541 ggctttaaaa aaagagtggg acaagtaata aatgaaaatg aaatagagag aacatatata
      601 ctagattcat acgatatgct agaacacttt agttatctac tacaaaactc agactccaat
      661 aatatagttc ttattaatga aataattgaa tatggtgaga aagctttaaa tataaagtca
      721 agtgattatc taagcttaac cttactagac catttagagt ttttacttaa aagatgtgaa
      781 aagaatcaat ttataaaaag tcctttggta tggaatgtta aaagatttta tccaaagcat
      841 tttgaaattg gaatgcatgc actaaaatta attggtgata gtaagggaat taatcttcct
      901 gatgatgagg cagtatcaat agcacttcat tttgtaaata tggaggtaaa taaagagtct
      961 catgatagta caattgtaga gttgagaact ttagcagata tagtttctat aataaaatat
     1021 cattttaatg tagagctaga tgaaacttca acaaattata tgagatttac tactcatcta
     1081 cagtacttta tccaaagaat tatgtcagat gaagcttcaa tagatgaaga taattctata
     1141 gatttatata aacaagtaag tcaactttat ccaaagtcat ttggaagtgt tcaaaagata
     1201 agaaaatata taaaatcaca atttaatgtc gatatatcta taagtgaaga aacttattta
     1261 atgttgcata taaatagggt gactcaaaga ctggaggcaa aatcagatga aatataaaga
     1321 tttaaatgaa aatataataa aacttatagg gggaaaagaa aatataaagt cggtagcgca
     1381 ttgtgttact cgtttaagac ttactttaaa tgatagaagt atcgctcaaa cagaagaaat
     1441 taaaaaatta aaaggagtaa ttgatgtagt ttctaatgat gttgcttatc aaattataat
     1501 aggtactcat gttgtagatg tatataacga gtttatgtct atgataggtt taagttcaag
     1561 tgaatcaagt ataaaaagtg aaagaaaaaa gggaattaga ggtatcaaag gtatatttaa
     1621 ttccatcttt gtggtagtct cagagactat gacttcagta attgaagtac tacttgcagc
     1681 tggtatactc gcaagtatat tagcactttt aaatatgtct ggcatacttc agagtgatag
     1741 ccctacatat caaatattag atacattaag aagtgctaca tttcacttct taccagtatt
     1801 aattgcaata gcatcagcaa aaagacttaa tgtaaatccc tatatagcag tagtgttagc
     1861 agttacgtta ttatcgtcat caatagatgg tgtagaagga ttaagtttat ttggtataaa
     1921 tttacaggct acaacttatg caaacagttt tattccaata cttttaggag tatgggcaat
     1981 gggaattata attgaaggtt tgaaaaaaat attacctaaa gcactacact attttttagt
     2041 tccagtgtta tctttaataa taaccctatc agttacatta actgtatttg gacctattgg
     2101 tatgtggatt ggtgatggat taaactatgc atgtatgttt ttaattgata cattgggtaa
     2161 ttggagtgtt gttgcactgt atgcagcttt acaaccactc ttaatagttt ttggtgcagc
     2221 aaactttact atgcctatat ctttaaattc tgtgacaaca ttggggtatg acccaatttt
     2281 tatgggcgct gcaacaatat ctgatattgc agtttgtggt gcaatgtttg gatatttctt
     2341 aaaaactagg aataaagaac aaaaacaatt atttgggaca gtaagtttta gtgcacttat
     2401 gggagttaca gagccttcag tgtttggtgt gtttatgaaa tatagaagac catttgtagc
     2461 agtaatgata ggtggaggta taggtggttt aatagcaggt ttagctcaag ttaaaacata
     2521 tggaatggtt tggggattag ctgcattacc aacatatctt gttggaggag aggcatctaa
     2581 ctttacattt atgataataa gtgtagtagt atcatttgta gcagcaacag cagcttccta
     2641 tttactagga ataccttcag atgaaaaaga agaagttaag ggtgataatg aaaaagaatt
     2701 agaactaata atcaacgaga acaattcaag aactattagt attggaaatg ttgcaaaagg
     2761 tcaggtaatt gcacttgggg aagttaatga caaagcattt tcctctggag cattaggtaa
     2821 aggggttgga gttataccta gtgaatcaaa atcaactgtt atatctcctg tagatggaac
     2881 tataactata gtatttccaa ctaaacatgc atatggtata gtgactgatg aaggattaga
     2941 aatattaatt catattggta tagatactgt aaacttagat ggaaaattct ttgaaagtct
     3001 tgtaacacaa aatcaaaggg ttaaaaaagg agatgcttta gcagtatttg aatctgataa
     3061 aattatagag gaaggctttg attctactat aataactgta gttacaaata ctagcgatta
     3121 tttagatgta atctcatcaa atgatgaatc agaagaatta ttaactgtaa tagtataaat
     3181 tttaggaggg aaaattcatg aatattaaag atacattttt ctggggagga agtattgcag
     3241 cccatcaatg tgaaggttca tgggatagtg ataacaaagg acctgcaatt atggattttg
     3301 taactaaggg ctcttatgaa acgccaagag taattactga taaaatagaa gaaaaattgg
     3361 attatccatc tcataatgga atagattttt ataatagata taaagaagat atagctttat
     3421 ttaaagaaat gggatttagt gcattaagaa tatctataga ttggtcaaga atttttccaa
     3481 atggagatga tgaaaatcct aatgaattag ggattaaata ctatgaaggt ctgatagata
     3541 cattaataga aaataatata gaacctattg taacactata tcattttgaa ttacctatga
     3601 atttagtaca taaatatggt tcatggaata acagaaaact tatggatttg tatttaaagt
     3661 acagtgagac tgtaataaga aggtttgatg ataaagtaaa gtactggatt acatttaatg
     3721 agatgaacca tattgaccct caaacagagc actcggatat ttttacttat atgatagcag
     3781 gtattaagta tagtgaaata gaaaataagg ttcaaacatt ggcaaatgta gggtataata
     3841 tgactttggc gggtgtaaag gttgcaaagt tggctagaga aatcaatccc aacaataaag
     3901 ttggatgtgt atttggacta aatccaattt atccatacaa ttgtaatcct agtaatgtgt
     3961 taaatgcatt tttagataat gatcgtgatt attatcaaat tgatgcaatg tgcaatggga
     4021 agtttcccca atataaatta aaagaatatg aaaaaaataa tataactatt gaagtgactg
     4081 atgaagacaa aaaagcattt aaagaaggta agttagattt tataggtctt aattattatt
     4141 tttcaagtgt atctactcca aaagaaaatt tatcaggtga taaatcttta tttggtggga
     4201 ttcaaaatcc atatttggaa caaagtaagt ggggatgggc aatagaccct ataggtttaa
     4261 gatttacctt gaactatatt tacagaaagt atcaattacc tatcttaatt acagaaaatg
     4321 gtttaggggc atatgataaa atcgaagaag atggaactat aaatgatgat tatagaattg
     4381 aatatttaag taagcatcta tctcaaatga agtctgcaat tgaagaggat aatgttgaat
     4441 gttttggata tttgatgtgg ggaccaattg atttagtcag tgcaactaca ggtcagatga
     4501 gtaagagata tggatttata tatgttgact tagatgatga aggcaaagga agttttaaga
     4561 gatataagaa aaaatcattt gattggtaca aagaagtaat aaaatctaat ggagaagttt
     4621 tagatttttc tacggatata gtagtagggt agtaataaga taaatagaag ttaggaacta
     4681 atgttaatta gtgtctagta aagcatttaa aatagagtat agggtcttta aagtaaagtg
     4741 tgtattctta ttaaaagtaa tggcaactaa aattactata aaaaggagaa tacacatttt
     4801 ttatgtttat acttaagtaa atatttagac atattcatag tataatttac tcattatata
     4861 aaaatagtat gatttactca ctatataact ttataaattt tctagaaaaa tgtcagaata
     4921 tattgacaaa aaaaagattt gaaattataa tctatattga gaatagtttg acaatataaa
     4981 aacaagtatt tttacaaata gtgaaattat ttttattgtc aatttaagaa taatttagga
     5041 ctactataaa gatgaaggta tagatgtcat tatatgataa atcatacttg tatagaaaga
     5101 attttaaagt atgcttatca ttgatttaat ataataaata atttttgttt ctagtaatag
     5161 ttcataggaa taagatatct aaattcgctt agaaatagtc taatttacaa aggaggggat
     5221 gcactagtag ataaaatttt tatataaaga agacttaaag tggagtatat tctgataata
     5281 ttggtttaat ggttatatta ttatttcgtt ttagatattt taagaataat ttaagacgtt
     5341 tgtaaaaatg tattctatat ctaatattta aataaatata agtcttccaa catgtatact
     5401 aagatagaaa gtagttgaag tacaattcta acttgtaaaa ttatgtagaa agtaaaaatc
     5461 aaaaagaaat ctagtaagtt gataattata gttaatattt agagagttaa ctctagttat
     5521 atttatttag atattttagg aataatttaa gacgtatata atatcatcaa aaaatatcaa
     5581 gctataccat atgtagttaa gaataactgc ataatataca ttttataatt attgttttat
     5641 tgataaaaaa ttattatatt taggaggaaa caacaatgga aaatttttgg aatccactta
     5701 cttgctttac tgtatgtgta atgatttatg cttttagtga gtttttatct aagaaaacaa
     5761 aaggtgcaat ttcaacattg ttatttgtat gtattatatt tttactagga ttttggacaa
     5821 atgttttacc aaaagacatt acaggagcat cagggatatt agctgcaatg agcaactttg
     5881 gtatagcttt attaataact aacttaggaa cacttataaa tcttgaagac ttatgtcatg
     5941 aatggaaaac ggttgtaatt gcattagtag gtattgtagg aataggggtt ggctgtttaa
     6001 cagtgggtag tatgttattt ggtagagagt atgctttaat agcagcacct ccaatagcag
     6061 gttctactgt tgccggtatt atcgtaacaa ctgttgcaga agctgcaaat agacctgaac
     6121 ttgctgcatt tgtaattcta gtattatcat ttcaaaagtt ctttggcatt ccgataacta
     6181 catattgtat aaataaagaa ttaaaattta aaagagataa cggggatttt ttgaaagatg
     6241 agtcaaaaag taattttaga cttccaagta tgagaatttt taaagaagat ttaggtaaaa
     6301 aatcatctac tctagtgtat cttgcaaagc tttcatttgt tgctatgatt ggtaattttg
     6361 taggattggc tactttaata ccaggattta cccctgcaaa ctattattta aatcctaata
     6421 tagcatgttt gttagtaggg ttgattttta cacgaatagg ttttttagaa aaaggaattt
     6481 tagataaatc tcaatctaat ggaattataa tgtttggttg tatgttgatg ttacctggag
     6541 gacttgctaa ggtaactcca acagcattgt ttgggatgat tgttccaact ataggaatac
     6601 ttgttgtagg ttctgtattt ataatattat ttactgcaat tgtaggaaaa ctattgggat
     6661 attctccaag aatgtcagct gcaattggtg taacttgtat gctagcttat cctgcaacac
     6721 agataatttc caacgaaggt attgatgcta tggaaggaac tgaagaagaa aaacaaaggg
     6781 caacagatta tttattacca aagatgatta taggaggatt tgttactgta accattgcat
     6841 cagtggcatt tgctagtata ataggtccaa ttatatttgg ttgatagtaa atatacttat
     6901 aggaggaata agaaatgata ggaagtatat ctataaatga gatagataaa aaaagattca
     6961 aacttgatga tttaagtaag aaaatatggg aaaacccaga aaaagcattt aaagaattta
     7021 aagcatgtga aaatacagca aactttctaa gaagtgaagg atttgatgtg gaagtaggtg
     7081 taggaggttt agccacggct attagagcta gttttggttc aggtaaacca gttataggtt
     7141 ttatgggaga atttgatgca ttacctggtt taaatcaaaa agtttcaaca aagcaagaag
     7201 catttgaatt gagtgcatat ggtcatggat gtgggcataa tttattatgt acagcacatg
     7261 taggggcagt agtaggactt aaaagagaga tgctagaaaa taacttaagt gggactatag
     7321 tattttatgc atgtccagga gaagaacaat taacaggtaa aggatttatg gctagaggtg
     7381 gagcatttga agggttagac ttagctataa attttcatcc aaataaaatt agtgaagcta
     7441 caattggtac ttcaacagct gttaactctg taaaatttca ctttaaggga aagactgcac
     7501 atgcaggctc agaccctcaa aatggaagaa gtgcactaga tgctgtggaa cttactaatg
     7561 tgggagctaa ttatttgaga gaacatgtta cttctgatgt aaggattcat tatacaatta
     7621 cagatggagg agtagcaccc aacatagttc ctgataaagc atcagtatgg tattatacaa
     7681 gagctttaag tagagaagct gtagaaagta catatgaaag acttgtaaag gtagcaaaag
     7741 gagctgcaat gatgactgaa actgaagttg aagtagagtt tttaggaggt tgttataata
     7801 ctttaaataa tcatgtttta gcaaatcttg tatcagaatg tatgaatgag attaaacaag
     7861 agccttggac tcgagaggaa ttggattttg cagctgaatt agatagacaa agcccacaac
     7921 aatataaggc tatgataagt aaatataatt tgccagaagg aactcatcta tattatggtg
     7981 gaggaaatgt tacaaacttt aatagttatg gttctactga tattggagat gtaatgcata
     8041 tagttccgac agcatacttt tttacagggt gtacgaatct aggtgcacca ggtcatagct
     8101 ggcagtttac atcttgtgca ggaagttcta taggagaaaa aggtatgata tatgcatcaa
     8161 aaatcatggc tatgtttgga gccaaaatat taaataatcc agagatagct aaaaaggcaa
     8221 aagaagagtt tgataaatct atgaatggta aaacatataa atgtcctatt ccagatgata
     8281 ttccaactcc ttaaaaagac atgaagttta atatataaaa ttataatttt tatatcagca
     8341 tcatttcatt actaaaagaa agtgagattt tgctgactat ctgattagga ggaaggaata
     8401 tgaaaaattt ttttaaagaa gcgcagtcaa tagaagggga aattattaga tggagaagat
     8461 atttacatga aaatcccgaa attggttgca atctaccgaa aacagtaacc tttgttacca
     8521 gtactttaga aaaaatggga tattctccga aattgacgtc taataatgga atagtagctg
     8581 ttttagacag tgaaaatggt ggaaagacct tattattaag ggcagatatg gatgcacttc
     8641 ctattatgga ggagacggat ttacctttta aaagtaagaa taaagatgca tctcatagtt
     8701 gtggacacga taatcacaca gcaatgctct taggagcagc taaaatactt atggataata
     8761 agcacttact taaaggtaga gtaaagtttg tatttgaacc agatgaagag agtaccagag
     8821 gtgcaaaatc aatgattgat gatggtatat tagaaaatcc tcaggtcgat gcagctatgg
     8881 catttcacag tatggtgggt aaatttctta acacaggtca agtagcttat tcacgtggtc
     8941 cagctatggc atctgcggat atatttgaaa taacagtaga aggaaaagca actcatggag
     9001 caagccctga atttggagtg gacccaataa acataatgtc gcatatacaa atagcattaa
     9061 acactatact ttcaagagaa aaacctcaga aagagcctgt agttttaact attggtatgt
     9121 ttaatgcagg aaacgtacca aatgctatac caaataaagc atctatgtca ggaactttaa
     9181 gaacatttga ccaatttgtt agagaaaaat taaaaaatag aattattgaa atatctacag
     9241 gaatagctaa atcatttgga ggaaatgcta aagtggaatt taaaatgggg actcccgcag
     9301 ttataaatga tttggatgta ggagatgaga ttgtatctta tgttaaagaa attataggaa
     9361 atgagaatgt aatagacttt ccatctgcaa tgggttcaga agatttttca gagattttac
     9421 taagggttcc aggagtattt ttatggattg gaatgggaag tattagtcag ggatataatt
     9481 ttggtatgca taatcctaaa gcaattttta atgaagaggg attagtttat ggttcagctt
     9541 tatttgctca ttgtgcagta aaatggttag aaaataattt tagtagataa taacataaaa
     9601 tattattaaa tatcataaaa ggtatatctt aaaaagatgc aaagatatac cttttagaaa
     9661 gtcatatata attttactta atattagatg ataaaatatt tttatgcatt ttggatttta
     9721 atatttattt tctgatataa ataatttgta taccctttca ggtctacctt ttgttgagtt
     9781 ttgtttttca tataatatag tagcgtttcc agactttaag agactactta gaaatctatt
     9841 agcagttcta actgttacat ttaaacatga agataattcc ttaggagtaa cttcatctct
     9901 ttgtagaatt tcagtaacag atataatctt ttgaatagta agagtagaca atttagaact
     9961 tttggcaatt gtatttatat gtggagttac tttatttgaa acagtaagta acttatcaca
    10021 cagtaaaggt cctattgtct cataattttc gttgactaag aaactgtaat ttaaaggatt
    10081 tattttagat tccttattag cacttatggc actagacata gcttgcttta tatcattacc
    10141 aattccatat ccaacacata ctttaatatt tgtactattt tcaatagatt cttttatata
    10201 acatgcagta aaatttttag ttaatgattt aatcgtttca catttagtga aaattttaaa
    10261 agaattacta tttttttcaa ttataaaatc ggttaattta tcttttttaa tactatttaa
    10321 tattttataa acttggtcta gagtagagtc atcataattt gtatctttaa ctgttatata
    10381 tattacactt ggttgatttc ccttcattct atgtaaatga atatctttta ttaagttatt
    10441 aaaagtattt tttaataaat ctatatcagg gtatatgaaa tgaaagttta ctcctgcatt
    10501 ttgtaacata ggaacaatac tactaaaacg agtagtagaa aaatcgattt tcccttcatt
    10561 ccatagatta atatgttttt ttgcaatctt tttttcaagt tttgctatgt cagataaact
    10621 cattttagat gcattttcat caatactatc cattaagtct ccaaatgtgc catttgataa
    10681 ataatatgat atatcttttg gattatctgc ccagtctgtt aagtcaaagt atgctctaga
    10741 aaaatctaat gtattatatt taaagataag ctttagaaac atatcatagt aactttgtaa
    10801 gtcagtacca aactgaacct cccatgacta aagtcacagg gttcctgctt catagaattt
    10861 tgcatactaa aatatgtctt actaattct
//
LOCUS       DAGQWR010000140        10726 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.141, whole genome shotgun sequence.
ACCESSION   DAGQWR010000140 DAGQWR010000000
VERSION     DAGQWR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.141"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            252..1430
                     /locus_tag="MIW95_003156"
     CDS             252..1430
                     /locus_tag="MIW95_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBY3152461.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINSGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            1439..2095
                     /locus_tag="MIW95_003157"
     CDS             1439..2095
                     /locus_tag="MIW95_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBY3152462.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            2095..2760
                     /locus_tag="MIW95_003158"
     CDS             2095..2760
                     /locus_tag="MIW95_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBY3152463.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     ILGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            2865..3641
                     /locus_tag="MIW95_003159"
     CDS             2865..3641
                     /locus_tag="MIW95_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBY3152464.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            3703..5037
                     /locus_tag="MIW95_003160"
     CDS             3703..5037
                     /locus_tag="MIW95_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBY3152465.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVLLNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKVGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSKLEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            5534..6460
                     /locus_tag="MIW95_003161"
     CDS             5534..6460
                     /locus_tag="MIW95_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBY3152466.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPNNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSENFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(6852..10391)
                     /gene="nifJ"
                     /locus_tag="MIW95_003162"
     CDS             complement(6852..10391)
                     /gene="nifJ"
                     /locus_tag="MIW95_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBY3152467.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCIDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         3729 a         1730 c         1663 g         3604 t
ORIGIN      
        1 ttttctaaat ctactataaa tttttatcct ataaattttt cttatacact atataaaaaa
       61 ttagacttag acaaaatgct tttttcgccg taatctataa ttagaaacag gaaaacctaa
      121 agcattagga ataaaagtaa ttgaaatact taaaaacaag cttgtattaa aaaagtactt
      181 tattatagtg ttccctaatt tctttattca gaaaattttt aattatcatt caagaaaggt
      241 aactattaga tatgaaagat gtagtaatcg tatcagcagt cagaacacct ataggaagtt
      301 ttggaggtgt atttaaaaat acttcagctg ttcaacttgg aactatcgct gttaaagaag
      361 cgatatcacg tgtaggactt aatttatctg aaatagatga agtaataatt ggaaatgttc
      421 ttcaaacagg gcttggacag aatgttgcta ggcaaattgc tataaattca ggtataccaa
      481 attctgttcc aagctacaca gtaaataaat tatgtggttc agggcttaaa agtgttcagc
      541 tagctgccca atcaatcaca tctggtgaaa atgatgtggt aattgcaggt ggtactgaaa
      601 atatgagcca agctccttat atcgttccaa ctgctcgctt tggtagtaaa atgggtgaca
      661 taaccatggt agatagtatg ctaacagatg gccttattga tgctttcaat cagtatcata
      721 tgggaataac tgcagaaaat attgcaacta aatttgaatt tactagagaa atgcaagata
      781 aattggcatt agaaagtcaa aataaggctg agaatgctat aaaaaacaac cgctttaaag
      841 aagaaatcgt tccagtagat gttttaatac gtcgaggaaa aatagaaact attgataaag
      901 atgaatatcc taaattaggc atgacatttg aaggtttagc taaacttaaa cctgcattca
      961 aaaaagatgg tactgtaaca gctggaaatg cctcaggtat taatgatggt gcggctatgt
     1021 taatactaat gagccaacaa aaagcagacg aactaggtat tagaccactt gctaaaataa
     1081 aatcctatgc ttcagctggt gttgaacctg aagtcatggg tactggtcca atccctgcaa
     1141 ctagaaaagc actgaaaaaa gctggactta gcatcaatga tatagactta atagaagcaa
     1201 acgaagcgtt tgctgcccaa gcattagctg ttaaaaatga acttcaaata gattcatcaa
     1261 agttaaatgt taatggtggt gcaatagcat taggacatcc aataggagca agtggagcta
     1321 gaatacttgt taccttaatt tatgaaatgc aaaaacgtaa agttgaaact ggacttgcaa
     1381 cactttgtat tggtggtggt caaggaatat caatggtggt ttcacgatag gaggaaatat
     1441 gaataaaata gttagcattg atgaagctct atctcatgta aaagatggag atacaataat
     1501 ggttggtgga ttcatggcca atggttcacc agaaaattta attgattatt tatgtagtaa
     1561 aaatataaaa gatttgacac taatttgcaa tgatactggc tttattgaca aaggagttgg
     1621 caaaatggtt gtaaataagc aatttaaaaa gataattgct tctcatgtag gattaaataa
     1681 agaaactgga agacaaatga acaacaatga aacagatgtt gagttagttc ctcagggaac
     1741 tcttgctgaa caaatacgtg cagctggtta tggtctagga ggtatactca caccgactgg
     1801 tattggtact cttgttgagg agggaaagca aaaaattgtt gtcgatagca aagaatacct
     1861 tcttgaaaaa ccaatttatg cagatgttgc tcttctattt gcatcaaagg tcgataaagc
     1921 aggtaatcta gtttataaag gctctatgaa caattttaat aatttaatgg catctgctgc
     1981 caaaattaca atagttgaag cagatgaaat cgttgaaatt ggtgacattg acccaaatga
     2041 agttaataca ccaggtattt ttgtgaatta catcgttgaa ggaggtaatt tataatggat
     2101 aaattagaaa tgcaagaata tattgcaaat agagtatcta aagaattaaa agacggagcc
     2161 gttgtaaacc ttggaatagg tctaccaacc aaagtagcaa attatattcc ggataatgta
     2221 aacgttatct tacaatctga aaatggcttt ttaggtttag gttcagccga agatggaaat
     2281 tcaagtgatg aaactattgt aaatgcaggt ggtcaaccag ttactatact tcctggagga
     2341 tgtttctttg atagtgctac ttcttttgga ataatcagag gaggtcatgt tgatataact
     2401 attcttggcg cacttcaggt tgacaaatat ggcaatatag caaactatat gattccaggt
     2461 aagatggttc caggtatggg tggagctatg gacctagtaa ctggagctaa aaaagttatt
     2521 gtagcaatgg aacatacttc aaaaggttct gcaaaaatat taaataattg tactcttcca
     2581 cttactgcaa caaatgctgt agatttgata gttactgaaa tgggagttat ggaagtaaca
     2641 tcagatggaa ttttacttaa agaaataaat ccagctttca cattagatga tgttatttct
     2701 gctactgaag ctcctttaat attgtcagat tcattaaatg gaaaaatatc tgttgtttaa
     2761 aataattaaa actagaaaat aaaaatctct tagacgtatt acatataaat ttgtattgct
     2821 taaaatatta atataaaaat aaaataaaat tggaggaaat gaaaatggtt aaagataaag
     2881 ttgtattcgt aacaggagct gcaagtggaa taggtaaaca aatagctgaa tcgttcttaa
     2941 aaaatggtgc taaagtaatg ttctctgata taaatcaaga agctttaaat aataccataa
     3001 cagagttaca aaaaaaaggt tatgattgca tgagtgtaaa gtgtgatgta actaaggaag
     3061 aagaaattaa ccacgctata gataaaactg ttgaaaagta tggcagatta gatatattaa
     3121 taaacaatgc tggacttcaa catgtttcta tgatagaaga tttcccaact gataaatttg
     3181 aatttatgat aaaaataatg ctgacagcac cttttatagc tactaaaaaa gcatttccta
     3241 taatgaaaaa acaaggcttt ggtcgtataa taaatatggc ctcaatcaat ggtgtaatag
     3301 gatttgctgg aaaggctgct tataactcag caaaacatgg acttattgga ctaacaaaag
     3361 ttgctgctct tgaagctgcc gattctggta taacagttaa tgctttatgc cctggatatg
     3421 tagatactcc acttgtcaga ggtcagttta atgacctttc aaaaactcgt aatataccat
     3481 tagaaaatgt cttagaggaa gttttgtacc cacttgtacc acaaaaacgc ctattagatg
     3541 ttcaagaaat tgccgactat gctatgttcc tagcaagtga taatgctaag ggtattacag
     3601 gacaagcttg cttactagat ggaggatata ctgcacaata gttattatat ttagggcttg
     3661 aagaaagtat tcttcaggtc cttctactaa ggagggatat ttatgagtat tatcgcacca
     3721 attggtataa ttattggtat catagctatt atatattttt cagttaaaga aattcatata
     3781 actatagctg ctccaatagc aactttaata gttgttctct taaataaaat ggatgttgta
     3841 acatcaatgc taggtgctga caaaaacaat tatatgggtg cacttggaaa ctatataatg
     3901 agttattttg caatattctt attaggctca atacttgcta aatttatgga agaaagtgga
     3961 gccactgtat caatagctga ttttatttta aataaagttg gctctaatca tccttataga
     4021 gttttagttg ctatatttat agtaagtttt atattaacgt atggtggtat aagcttattt
     4081 gtagttatgt ttgcagtcat tccacttgca agaactctat ttaaaaaact tgatatttct
     4141 tggaacctta ttcagatacc actttggctt ggtattgcaa caattactat gactataatt
     4201 cctggaacac cagccattca aaatgttata cctattcaat atctaaatac gtctcttact
     4261 gcagctacta taccaagtat tgtaggtagt ataggttgtg ctacgtttgg acttgtctat
     4321 atgaaatatg ctctaaacaa aagtattaga aaaggtgaaa cttacgcaac ttacacttca
     4381 aaattagaag aagaatcttt agatagagaa ttaccaggat tttttgcaag tatttttcct
     4441 ctattagcac tcgtaattat agctataact ggaagtactt ttggttcaga atttatgaaa
     4501 aaaaatgtaa tttacatagc tttaatagtt gcaattcttt tatctgcttt tttattccat
     4561 aaatttctat catctaagat acagacattg agcataggtg ctagtggttc tataggtcct
     4621 atatttgcta catcttctgc agttgcattt ggagcagtaa taatggctgc acctggtttt
     4681 gatgtattta gcaaacttat aatgtcaata ccaggtagtc cacttataag tcttactgtt
     4741 ttgacttcaa caatgtcagc aataacaggg tcctcttctg gtgcattagg aatagtaatg
     4801 ccaaactttg cagattatta tttaagtact ggtctaaatc cagaattaat tcaccgtgtt
     4861 gctacaatag cctcaaatat actaactata gttccacaaa gtggtgtttt tcttacattc
     4921 ttaagcttat ctaagttaac acataaaact ggatttaaag aaactttcat tgtagttgca
     4981 gttggttcac taatagcaga aattattgta attacattag gattactaat gtattaatat
     5041 aaaacaaatt aaaattcata atatgaaagg tggtgtctca aagtgaactt tttacttaat
     5101 gagacactct ttttgtatga aatcaaatat attttaatca tttcaacaat aaaaaagaac
     5161 ctttctctat tttgagacaa acccttttta ttccttaaag atttaaacaa ttataatttg
     5221 ttactaatac gaaaacatct attgaataaa tttaaccaaa taaaaaaaaa tttatcataa
     5281 ctataaattt acgttatgga attattataa aaatattatt ttctctaagt aagtaattcc
     5341 atactataca ggcttttaat acaaattttc aaacacactg aaatatacct atttattcca
     5401 tatatatctt ttttacatat ctttacagta aaaaaagtct taatcttatg ttagtataga
     5461 ttcacgggta ccccctacac taaaggttac aaatcagtaa catttttaga atttttagat
     5521 taagaggtgt ttaatgatga gaagaaatat accaacaaaa ttaaaaacta tattagcttt
     5581 aggagtgtta actttagcaa cagcttcaag tgctgttact gcaagtgctt catcacttga
     5641 aaataatgta aatataagta acaaaagttt agtatatagt aatactaaat acaagttttc
     5701 tgcaaaacca aattgttctg taaaaccaga acttaaacct gataaagata actcttgtaa
     5761 ggataagaat cataatgaca agaattgcaa caacactaat aaacctgagg acaacaataa
     5821 ttcagactct actaataagc ctgataacaa taaaccagag tctaataaac ctgaggacaa
     5881 caataattca ggctctacta ataaacctga taacaataaa ccagagtcta ataaacctga
     5941 agacaacaat aactcaggct ctactgataa acctaataac aataaaccag agtctaataa
     6001 acctgaagac aacaataatt cagactctac taacaaacct gatgataata acaactcagg
     6061 ctctacaagt gaaaattttt cagcttacca aaaagaagtt gtcgacttag taaatataga
     6121 aagagctaaa gctggattaa atcctcttac tcttgattct tctatatcaa atgttgctac
     6181 aaaaaaatct caagatatga ttgataacaa ctatttttca cacaattctc caacttatgg
     6241 ttctccattt gacatgttga aaaaatttgg aataagctat aagacagcag gtgaaaatat
     6301 agctatgggt caaaaaactc ctaaagaagt tgtaaacgca tggatgaatt ctgaaggtca
     6361 cagaaaaaat ataatgaatc caaatttcag taaaataggt gttggagtag cacaaaaaag
     6421 tggtggttca atttattgga cacaaatctt tgtaggataa aatactcttt ttccatgcat
     6481 atcaaaagag tatctctaag tgataaaaaa cacaaatgag atactctttt gtatctattt
     6541 taaaattatc aaatccaaat atacatacta taaatattat ttgttctctt aattttagtg
     6601 tgtagaacaa tatataaaaa gtattggcaa gatgaaaatg gaaaacttaa aaagatattt
     6661 aataggtaaa aacaggatct taatatcttc ataaaaaaag gctgtctcaa aatagagata
     6721 aggccttttt cattgttgaa atgattaaaa tatatttgat ttcatacaaa aagagtgcct
     6781 catgaactaa aaagctcact ttgagacact ctcttttatc aattctatat aagcattata
     6841 atactttaat attattcagc taattttttg tatccttcat atctttcttt agcatcagct
     6901 tcagctttag caaataatcc atcagcttcc tctgggaatt gtttagctat agcagaatat
     6961 cttacttgtg caagtatgaa atctttgaag cttgcagttg gttcttttga atctaaagtg
     7021 aatgggtttt taccttcagc ttttacttct ggattgaatc tgtataaatg ccagtatccg
     7081 caagcaacag cttgtgcttc attagccaca gttcttccca taccttcttt taatccatgg
     7141 tttatacatg gagcataagc tattattaga gatggcccat catgtttttc agcttctaaa
     7201 acggctttga taaattgatt tttatcagcc cccatagcaa cttgagctac atatacgttt
     7261 ccatatgtca tagccatcat tcctaaatct ttctttctag attttttacc tgaagcagca
     7321 aacttagcca tagcagcagc tggagtagct ttagaagctt gacctcctgt atttgaatat
     7381 acttctgtat caaatacaag tacgtttaca tcttcaccag atgcaagaac atggtctaat
     7441 ccaccgtaac caatatcata tgcccaaccg tctccaccta gtatccattg agatctctta
     7501 actagataat cttttctatc ttttatagct tttactaatt ctttagcttt ttcatctata
     7561 caatcacaag ctggtttttc taataattca acaaccttag cacttgctac tttagaagct
     7621 tctccatcat ttctagaatc taaccactct gtaaatactg ctttagcatc ttcacagata
     7681 tccatagaaa ctaactctgt catagcatct actattttat ttcttatttg cttaacagct
     7741 gtgtacatac caaatccata ttcagcatta tcctcgaata aagaatttgc ccaagatgga
     7801 cctttacctt catggttaca agtataagga attgatggtg cagaagctcc ccatatagaa
     7861 gaacatccag tagcattagc tatcatcatt ctatctccat ataattgtgt aactacttta
     7921 atgtacgcag tttctccaca tccagcacat gctcctgaaa actccattaa tggttgtcta
     7981 aattgacttc ctttaacagt atttgcaggc ataacatcaa cttttggagc tacttcatca
     8041 ggatttactg caaatgccca attatctaac tctgtatcaa gttgagtatc tattggcttc
     8101 ataactaaag ctttttcttt agctggacat atgtcagcac agtttccaca tcctgtacag
     8161 tcaagtggac taacttgaat tctatatttt aatccttcta atcctttacc taatgctttt
     8221 ttagcttcaa atccttctgg agcattagct aattcttctt cagtaactaa tactggtctt
     8281 atacatgcat gaggacatat aaaagaacat tggttacatt gaatacagtt atctattatc
     8341 cattcaggaa catcaacagc tataccacgt ttttcataag ctgcagtacc acaagggaat
     8401 gttccatctt ctataccatt gaatgtactt actggtaagt tgtttccttc ttgtgcagtc
     8461 attggtctta gtatattttt tataaattct ggttctttaa cttctccttt atctgcatca
     8521 acagcatttt cccaagaagc tggaacttca actttaacta aagcatttat tcctgcatca
     8581 acagcagcat agttcatatt aactattttt tcaccttttt taccataagc tttaactata
     8641 gaatctttta agtatttaac agcatcttct tctggtatta tttcagctaa tttaaagaat
     8701 gcagattgca ttatcatgtt tattctgttt ccaagaccta tttcttgtcc tagtttaaca
     8761 gcattaactg tatagaattt tatatcattt ttagctatat attgtttcat atgagctggt
     8821 aaatgttctt caagtcctgc ttggtcccat atagtgttaa gtacaaatgt accacctttt
     8881 ttaagacctt ttaataaatc atattgataa acatatgatt gtttatgaca ggcaatataa
     8941 tcagcttcat ctattaaata agtagctctt attggagtat caccaaatct taagtgagac
     9001 atagttatac caccagattt tttagagtca taatcaaagt aagcttgagc atatttttca
     9061 gtatggtcac cgattatttt gatagcttgt ttattagctc caacagtacc gtcagaacct
     9121 aatccccaga acttacatct tatagttcct ttagaagcta tttttattgg ctcagatgga
     9181 gttaaagaag tattagttac atcatcaact atacctactg taaatccatt tttaggttgt
     9241 tctgaaacta agttatcaaa aacagttttt atatctgaag gagtagtatc ttttgaacct
     9301 aatccgtatc ttccacctat tatcatagga gcattttctt ttccatagaa tacatcacgt
     9361 acatctaagt ataatggttc accagttgaa cctggctctt tagttctatc aagtacacat
     9421 attctttcaa cagtagatgg catagcatct aagaaatgtt tcattgagaa tggtctatat
     9481 aaatgaactt taactaaacc atatttttct cccttagcat ttaagtaatc tatagtttct
     9541 tctattgttt ctgttactga acccatagct attaatacgt atttagcatc ttctgctcca
     9601 tagtaatcaa ataaactatg ttttctacct gttaaattac tcattttttc catgtatttt
     9661 tcaactatac ctactatatt ttgatagtat ttattagaag cttctctagt ttggaaatat
     9721 atatctgggt tttgagcagt accacgagca actggatgat ttggagataa agcattatct
     9781 ctaaatgctt gaactgcttc aaagtttaat aaacttgcat aatcttcatt ttctagtaat
     9841 tcaacttttt ggatttcatg tgaagttctg aatccatcaa agaaatgtat aaatggtaat
     9901 ctaccttcta ttgcagctaa atgtgcaaca ggtgctatat cagctacttc ttgaactgaa
     9961 ccagaagcta ataatacaca tccagtttgt ctagcagcca taacgtcttg gtgatcccca
    10021 aatattgata acgcttgaga tgccaaagca cgagcactta catggaatac tcctggtaat
    10081 aactctcctg caactttata catgttaggt atcattaata ataaaccttg agatgctgta
    10141 aaagtagaag ttaaagctcc tccttgtaaa gaaccgtgga atgttcctga tgcacctgct
    10201 tctgactgca tttcaacaac attaactgtt tgtccaaata tattttttct tccttgtgat
    10261 gcccactcat caactacttc agccatagtt gaagatggtg tgattggata tatagctgct
    10321 acatctgtaa acgcataggc aacatgagct gcagctgtat ttccatcaag tgttttcata
    10381 aacttagcca taatatgtat cctccttgat agctacaatt gtacttgttt ataacaagtc
    10441 tattttttta tatttacata taatttacac aacaaaaata taaacacaca tatagtcgcg
    10501 caaatactct atcttctaat ataatactac agattagata aaattccttt gaaaattgta
    10561 aattaattac atatttaagt agattttttt taaaattaaa tatgttaagt ttttttcata
    10621 attttgtata caatatataa taaattaagt tagacttttc aaactttttt atctatttgt
    10681 ttacattagt ataaaaagta aaataattat ctttattata tgtaaa
//
LOCUS       DAGQWR010000141        10637 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.142, whole genome shotgun sequence.
ACCESSION   DAGQWR010000141 DAGQWR010000000
VERSION     DAGQWR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.142"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            132..1316
                     /locus_tag="MIW95_003163"
     CDS             132..1316
                     /locus_tag="MIW95_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152468.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(1693..2802)
                     /locus_tag="MIW95_003164"
     CDS             complement(1693..2802)
                     /locus_tag="MIW95_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY3152469.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            3265..4458
                     /gene="nifV"
                     /locus_tag="MIW95_003165"
     CDS             3265..4458
                     /gene="nifV"
                     /locus_tag="MIW95_003165"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBY3152470.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVI
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            4483..6408
                     /locus_tag="MIW95_003166"
     CDS             4483..6408
                     /locus_tag="MIW95_003166"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBY3152471.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSNVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            6430..7425
                     /locus_tag="MIW95_003167"
     CDS             6430..7425
                     /locus_tag="MIW95_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBY3152472.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGAEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            7752..8225
                     /locus_tag="MIW95_003168"
     CDS             7752..8225
                     /locus_tag="MIW95_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY3152473.1"
                     /translation="MVGSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            8259..8753
                     /locus_tag="MIW95_003169"
     CDS             8259..8753
                     /locus_tag="MIW95_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3152474.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            8850..9371
                     /locus_tag="MIW95_003170"
     CDS             8850..9371
                     /locus_tag="MIW95_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY3152475.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMYDDNIEADKYIREELKVPSKYSVECIISIGYSDEEKKAYND
                     SDLDYKKVHFNNF"
     gene            9560..9871
                     /locus_tag="MIW95_003171"
     CDS             9560..9871
                     /locus_tag="MIW95_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBY3152476.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            9899..10585
                     /locus_tag="MIW95_003172"
     CDS             9899..10585
                     /locus_tag="MIW95_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY3152477.1"
                     /translation="MKYSYKKCIIDSILLMFIIQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         4049 a         1169 c         1938 g         3481 t
ORIGIN      
        1 aggtagaaat aagttgaaaa tgtaaagtca ttggaaaaat atagttgaat ttaatgaaca
       61 aaaatgttat tgttttatca atgaagtgtt aaaataaaaa ctttcaatat cattttaggg
      121 aggatgattt catgcaaaaa agtgtaaaga aagcaaaagt aacgggatct atgttagcca
      181 tttttggtgt tgcaagcgtt ctttttagtt cacatgcagg aggaggattt gctacaggga
      241 atcaagaaac tcagtactat gtgcagtatg gatggacagc accacttatg gcaattttag
      301 caatgattat acttactgct accatgagag aagttatcat aatgtataat aataacaatt
      361 gtaggaatta taaggattta ttttgtgagc tttggagacc atatccaaaa cttgaaataa
      421 tatgggagat atattactat ctaatggttt taatagctgt aagtgctgtt atagcaggtg
      481 cagcagcagt atttcaaagt ataggagtaa attattttgt agctgtattt attattggag
      541 ttgtacttct tgtctttact atatttggtg ctatgcttgt atctaaagca gctacagcaa
      601 tgacaatagc tattctagta tgtactctta ctatttttat tgttggtata aaagctaaag
      661 taccagaaat aactgaaata ttatcaaata gaacgagttt cacacctggc tatatcaagc
      721 caatacttaa tacttttatt tatgcaggtt ttcaatcggt tgttatacct acacttgcag
      781 gatgttcaag accacttctt aagaactcaa aagaagctac taaggcaatg ataatttcat
      841 ttgttatgaa tgcaattgca ttaggacttg ctgtaactat gctaatggga tggtatagag
      901 aaattattgc tgctgggcaa acaacactcc ctactcttta tgttgcaggt caatctggaa
      961 atcatactat atacataatt tataatgttg cattattcct ttgtttaatg tctacaggag
     1021 taactactat atttggtctt gtaaatagat ttgaagacca taaagcactt tctttccttt
     1081 cttcaagaat gaaaagaagg gtatttacag cttgtgcaat aatggtagtg tcaatgctta
     1141 tatcacttac aggattaagt aatattgtta aatatggata tggatattgt ggttatcttg
     1201 gtctattcac tatagtaatt ccattcctta cactaggaca ttataagaat aagaagtttg
     1261 caaaggaaaa tccagaagct aaatggccag cggaattaaa tgaaaatgta aattagatgt
     1321 agtagcaaaa aatattgcta aagagaatac tataaataag aatactatag ataatatatc
     1381 tgcatgttta ttaagattat ttgcaaaatc taatattgaa caactataaa caagcaatat
     1441 aaaactaaga ataaaagata aaaagagggt gtctcaaaat ggacttttag tttataaggc
     1501 actctttttt atatgaagtc aaatatattt tcattatttt gacaataaaa gagactaatc
     1561 ctactttgga tagcctcttt tttctttaat ataaaatagt ataacagcaa agtatagatt
     1621 gatacatgtt ttctgtagct ttctatatat tacagtgttt gtgtggcatt ggtattttaa
     1681 gcaatctata aattataatt ctataattct tctactaatt ttagattcta tagccgtctc
     1741 tatttcactt aacattcgtt catttttagg gtcaataaaa gcacatgtat agccttcttc
     1801 accagctcta ccagttctac caatacggtg gatgtagtct tctggtgttt ctggtaaatc
     1861 gtaattatat atatgactta ctccagatat atcaaggcca cgagaagcta catctgtagc
     1921 tattaagtat tgtatatcta aatttctaaa tgatttcatt attctttctc gttttgattg
     1981 agcaatatca ctatgaagtt tttgacaatt atatcctctt atagcaagag cttcttcaag
     2041 attgtcaacc cttctcttag tcctacaaaa tattattgcc ataaaaggat tatcttcatc
     2101 tagtacctta cataaggcat ctaatttttt tctatctgta gtttctacta cttcttgttt
     2161 tatattatta agagtgattt cttctttttg aatagaaact acaacaggat tttttgtgta
     2221 tctataagct aattttttta cttgagaatt tatagttgca gaaaagcata gtgtttgctt
     2281 tttgtttgaa gtctctttta aaatagcttc cacttcgttt ttaaatccca taagcaacat
     2341 ttggtctgct tcatctaaaa caaaagtctt taacttacta aggtctattg tttttctatt
     2401 tagatggtct aaaagcctac ctggtgtagc tataatcata tgaatatttc cttttagttt
     2461 ctttatttga gaaccaatat cttttcctcc atatgctgca agaatactta catctttaga
     2521 ttctttaagt ttcatagctt cttctgttat ttgaatagct agttctcttg taggagaaag
     2581 tataagtact tgtatatcat ttatatctaa tgatatattt tcaaatatcg gaagtaaaaa
     2641 agctagtgtt tttcctgttc cagtttgagc ttctgctatt acatcttttc cttcttttat
     2701 tagtttaata ctttcttttt gtataggtgt aggtgttgtt attcctgatt tttttagaat
     2761 atttattgta ttttcactaa tccctaagct tttgaaatcc attgttatct tacctttcat
     2821 gaaaaatttt cttttttatt aaccaattaa taatatcata tttgtaagta tttttagtaa
     2881 attttttaat tattatttat atagcttaaa aatatattaa gatactttaa aatggaatgt
     2941 aacttaactt aaattttagt aggaatttta taggtttatt tttaaaggtt ttttgaaaat
     3001 aaatctattt aaaaattaaa ttaaaatgca agaaaaacat aataactttc atttttaaga
     3061 agttttcaca aaaaagaaaa aatatagtgc aataaatgat acaaagtaat ataatagatt
     3121 acaagatatg aaaagctaaa aaaataaatt gcaaatgaaa ttaaaatgaa taaaaaataa
     3181 aaaaataatc ataagttaga ggtatcaatg aaaaataaga cttaatgttt ttaaaattaa
     3241 aagtatacta aggggggcat taatatgtgc gttatatcaa aagatagagc aaaggaaata
     3301 aaaatagtag atactacttt aagagatgga gagcaaacag ctggggttgt atttgcaaat
     3361 agagaaaaaa taatgattgc tgaaatgtta agtgacctag gtgttgacca aattgaagtt
     3421 ggtataccta ctatgggtgg agatgaaaaa aatgtaataa agcacatatg tagtagaaat
     3481 ttaaaatcag atataatggc ttggaataga gcagtaataa aagatgttga agaatctatt
     3541 tcttgtggtg ttgatgcagt agctatatca atatcagtat ctgacattca tatagaaaat
     3601 aaattgagaa cttctagagg ctgggtttta gaaaatatgg ctaaaacagt tgaatttgca
     3661 aagaaaaatg gattatatgt ttcagtaaat ggagaagatg cttcaagagc agatatagac
     3721 ttcttaactg aatttataaa tgttggaaaa caagcagggg cagatagatt tagatactgt
     3781 gatacagtag gagttatgaa tcctttttct ataaagaatg caatagaaac tctttatgaa
     3841 agaactaatt ttgatataga aatgcataca cataatgact ttgggatggc aactgcaaat
     3901 gcacttgcag gaatagcagc aggagcaaat tatgttggtg ttacagtaaa tggattaggt
     3961 gaaagagcag gaaatgcagc attagaagaa gttttaatgg cacttaagtg tgtttataaa
     4021 tgtgatttaa ataatataga tactagaaaa tttagaggaa tatgtgagta tgtagcacag
     4081 gcatcaggaa gaatacttcc tacatggaaa ccagtagttg gggataatat gtttatacat
     4141 gaatctggta tacatgcaga tggagcttta aaagacccgc ataactatga accatttgac
     4201 ccaagtgaag taaatcttga aagaaaaata gtaataggta aacattcagg aagagctgca
     4261 gttataaata agttgagtga atatgaaatg tatatttctc cagaaaacgc taccaagcta
     4321 ttaaatgcta taagagctac atctataaga ttaaaaagaa gtttaatgga taaagaaatt
     4381 cttcaattat attgtgatat actagcacat gagaaaggca caacagaaga agaagcagtt
     4441 agaggttcat atatttaaga tataattgtt taggaggaaa atatgggaga taatatagtt
     4501 tataaaataa ttaaaaaaca tatagtagat ggagaagctg ttgctggaag ttcaattggt
     4561 ataaaaatag accaaacttt aacacaagat tctactggaa caatgacata tcttcaacta
     4621 gaagctatgg gaatagataa agtaaagact aaaagaagtg tggcatttgt agaccataat
     4681 atgttacaac aaggatttga aaatgcagac gaccataagt atatacaaac agtggcagat
     4741 aaatatggag tttatttctc taagccagga aatggtattt gtcatcaagt atttttagaa
     4801 agattttcaa caccaggaga tacactttta ggttctgata gccacacacc tactgctgga
     4861 ggtgtgggca tgatggctat tggagctggt ggattagatg ttgctttagc catggcagga
     4921 ggagcatact atataaaagc accaaaagtc tgtaaggtga atctagttgg aaaattaaat
     4981 aatatggtat catcaaaaga tataatatta gaagtgttaa gaaaacaaac tgtaaaaggt
     5041 ggagtaggta aagtttatga atatggtgga gaaggtgtca aaagcctatc tgttccacaa
     5101 agagctacaa taacaaatat gggggctgaa cttggagcaa ctacttcaat cttcccaagt
     5161 gatgaaaaaa cactagaatt ttttaagagt caaggaagag aagacgcatg gatagagtta
     5221 aaaccagatg cagatgcagt ttatgatgaa gagattacta taaacttgga tgaattaaaa
     5281 ccattagctg caaaacctca tagcccagac aatgtagatg aagttgaaaa tataggcaag
     5341 attaaaatag accaagtggc aataggaagt tgtacaaatt cttcttatga agacttaatg
     5401 aaagtagcac aaatcctgaa aggaaataaa gtacataagg atgtgagttt ggtaatagca
     5461 ccaggttcaa gacaagttat ggagatgatt gctagaaatg gggctttggc agatataata
     5521 agtgctggag ctagaatact agaaaattct tgtggtccat gtattggtat gggtcaatct
     5581 ccaggtactg actctgtgtc tttaagaact ttcaatagaa atttctatgg tagaagtggg
     5641 actttgtcag cacaagtata tttagtaagt ccagaagtgg cagcagtaag tgcaatcaag
     5701 ggagtgctta cagacccaag agaatttgat attaaattta caaatttaga tgtaaatgaa
     5761 tttttgatag atgatagcat gataattaaa cctgctgatg taggaagtaa tgtagaagta
     5821 gttagaggac ctaatataaa acctttccct cttaatacag agctaagtca gtctataggt
     5881 ggaaaagtta tattaaagac agaagataat ataactactg accatataat gccatcaaat
     5941 gctaagttac ttccttttag aagtaatata ccgtatcttg caaactattg tttcaatact
     6001 gtagatacag aatttcctca gagagcaaaa gacaataatg gtggatttat agttggtgga
     6061 gataattatg gtcaaggttc aagtagagaa catgcagctt tagcaccatt atatctaggt
     6121 gttaaagggg taatagttaa gagctttgca agaatccaca aggctaactt gataaatagt
     6181 ggtataattc caatggagtt ttgtgatgag aaagattatg aaaatatatc tcttttagat
     6241 aatctggaga taccaaatat tttagacaat ctaggtagtg gaattttaga agttaagaat
     6301 acaactaaag gaacttcttt taaggtcaaa gtagaattgt cagcaaaaga agttgacgta
     6361 ttaaaagctg gtggaaaatt aaactacact aaaaatcaag ctaattaata tgaaaagagg
     6421 gaatattgaa tgtataaagt gacacttata ccaggagatg gaataggacc agaagttgcg
     6481 aaagctatga aaaaggtagt agaagctact ggagcagaaa tagaatggga agaagttaat
     6541 gcaggagagg cagttattga agagtatggt actccacttc ctgaatatat tatagatagt
     6601 ataaaaaaga acaaaatagc tataaaaggc ccaattacta ctcctgtagg taaaggattt
     6661 aggagtgtaa atgtaacttt aagacaggca ttagatttgt atgtcaattt aagacctatt
     6721 aaaagtttta aggggataaa atcaagatat gaagatgttg atttagtggt tgtaagagaa
     6781 aatacagaag atttatatgc tggaatagaa cataaaatag gtgattatgc agctgaaagt
     6841 ataaagataa taacaagaag tgcaagtgaa agaatcgttg attttgcatg taactatgtt
     6901 aaagataata agagaaaaaa agttacagct atacataagg caaatataat gaaaatgtct
     6961 gatggattgt ttttagatgt gtttagagaa gttgcatcaa aacatggtgt agaatatgat
     7021 gatttgattg tagatgcagc agctatgaac ttagttttaa atccagaaaa ttatgatgta
     7081 atggtaatgc caaatcttta tggagatata ctatcagatt taggtgctgg gcttgttgga
     7141 ggtttaggta taattccaag cgcaaatatt ggtaaagatt gtgcgatatt tgaagctgtt
     7201 catggaagtg ctccacaaat agcaggtcaa aataaggcta atccaacagc acttattcaa
     7261 tcatcagtaa tgatgcttag atacttaggt gaatatgaaa atgcacaaaa aatagaaaca
     7321 gctttagaaa aagtatttct tgaaggaagt aaattgactg tagatttagg tggctcagct
     7381 tctactacag agtttgcgga tgaggtgtgt aagtatattg tatagatttt atttagggaa
     7441 aaagtgaaat atagattgaa ggaaagaggc tttctcaaaa tagagataag ccttttttta
     7501 ttggaatgat aaaatatatt tgattttata caaaagagag ttcctcataa acgaaaaagt
     7561 taattttggg atatccattt taattttaaa attaattgat taatacgcaa agaaagatta
     7621 aacaaattta ttatttttaa ttgaactttc aaccaaaata tggtaaaatt taaaaaagtt
     7681 gaactttcaa ctaaaaataa atatattgat tatgtgaaat tctataaaac agaaactatt
     7741 tgactttagt catggtaggt tcagtggagg tgtgtttgat taagacttta gatagtaata
     7801 tattaagaga agtaggtact ttatctagag cagtaaattc aataaatgat attaagtaca
     7861 aggaattaaa gttacaaaaa ggacaattta catttttgac aagaatctgt gaaaatcctg
     7921 gtataaatct tgtggaatta tcaaatatgt taaaggtaga caaggctaca acaaccaagg
     7981 ctatacaaaa actaataaag gcaggatatg tagataaaaa gcaggatgag tttgataaaa
     8041 gaggatataa tttaactcca actgataaat ctttggaagt ttatgagttg ataattgaag
     8101 aagagaatag aagtatagag atatgttttg ataattttac agatgaagaa aaacaagtgg
     8161 taactaagtt attagaaaaa atgagcaaaa acgtagaaaa tgaatggttt aaagtaaaga
     8221 gatagtaaat aaatacatta acttaaggag atatttttat gattagaaaa gcgaatatga
     8281 atgatttaga aagcattatg aaaattataa aatctacagt ggaagaaatg aagacttata
     8341 ataatacaca atgggatgaa aactatccat tagaaaaaga ctttgtttca gatattaaga
     8401 aacaagattt atatatttat gaagtggatg gagaagtagc aggatttata tgtcttaatt
     8461 atgaagagcc agaagaatat ttaggcttaa attggtcatc taataaaaaa gcaatggtaa
     8521 tacatagaat ggctgtaaat ccaaacttta gaaagacagg aatagcatct aagttagttg
     8581 attttgctga aaaattagct gtagaaaaca atgtatcata cttaaagagc gatacatatt
     8641 caataaactc aaaaatgaat ttattactta caaagtgtgg ttttataaaa atcggagaaa
     8701 tgagtttttt aggaaaggag aaatctttct actgttatga taaaatatta taaagagcat
     8761 ttataataca aggggtaaat aatagatttt cagcattagt aagggcataa tataacccag
     8821 tatatgctat tatgaaaggg atgatttata tgattggatt tctaaagaaa agaagaagta
     8881 ttagaaaata caaggatgtt gaagtagaaa aagaaaaatt agataaaata ttaaaagctg
     8941 ctcttttagc tccatcatca aaggggctta gaacatggga atttatagtg gtagatgata
     9001 aagaaaagct aattaattta tctcaatgta gaactaaggg tggtggattt ttcttaaaaa
     9061 atgcaccttt agctattgta attattgcag ataaagaaaa aaatgatgtt tggatagagg
     9121 atgcaagtat agctgcttca tacatacaac ttcaagctca tgaactaggt ttaggttctt
     9181 gttggataca agtaagaaat agaatgtatg atgataatat agaagctgat aagtacatta
     9241 gagaagaatt aaaagtacct agcaagtaca gtgtagaatg tataatttct ataggatatt
     9301 ctgatgaaga aaagaaagca tacaatgact ctgatttaga ctataaaaag gttcatttta
     9361 acaactttta gtatataata tgttgtatta agacattata tttttttcta ttgttaatat
     9421 atacttaaaa ttgtaaaaat atatctgaat tttactaact tatctagggg ttatgtacaa
     9481 ggattttaaa gataaatata gaaattaatg tttataatcg atacataact tgagtaacaa
     9541 aatttaagga ggttaaagta tgggtatatt taaaacaaaa atggatgagg actggaaagt
     9601 aaactacatt aaagagttta atgaaatgag agattcttat gagagtaaac ttcaaaaaaa
     9661 acagtttgag gtagatagtt tgaaatcaga attggataga ctaagaagct ataaaaatag
     9721 ccttaagcca aaagaaaaac aaattactga tgatgatata aataatataa agagtttaag
     9781 aagagatggc ttaagctata aagaaatttc aaatcaaaca agttggagca aagcaactgt
     9841 aagtagggta ttaaatggac tttacgatta gcatatacat ggggtgattt aaattgggat
     9901 gaagtatagt tacaaaaaat gcataataga ttctatttta ttgatgttta taattcaaat
     9961 attaaggatg attttgaatt atgtacttct aagtcaattt gaatttactc ttgaaaattt
    10021 taatattata aacttaattt cttttacatt agttggttta tcgttaattt tatttttaaa
    10081 agataattct ttgtataaca aggttagaaa tagaaaaatc acagaagctt ttgaagaaaa
    10141 taaaaacaat atacttattg aaaagtgtaa gcttatactt tttgttgtgg ttttatcttt
    10201 agcaataatt gtaacttatt gtactaaggg atatgtactt tttaatgtaa ctatgatgac
    10261 tttgtctgta ttgatagttc caatatttga agagttattt tttagagaat atatatggaa
    10321 ctatctaagt aattttataa agtcaaagag taaaattatc tgtataacat ctatactatc
    10381 tggaatttat aatataggat acatagatgt tataagaaat tatgtaatat tgtataacaa
    10441 ttcaagctac acctttgaag ttattatttc aaaaattatg ataggtactg tttttggtat
    10501 tgtacttgga ttagttaagt ataggtttag agatgtaggg ttttgtatat tattgaggag
    10561 cttatttgct atatttatta ggtagatagt ataatcggtt tataaggttg ttttaaattt
    10621 aattttaaat caacctt
//
LOCUS       DAGQWR010000142        10567 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.143, whole genome shotgun sequence.
ACCESSION   DAGQWR010000142 DAGQWR010000000
VERSION     DAGQWR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10567
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.143"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            115..990
                     /locus_tag="MIW95_003173"
     CDS             115..990
                     /locus_tag="MIW95_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HBY3152478.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     RGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            1008..2498
                     /gene="cls"
                     /locus_tag="MIW95_003174"
     CDS             1008..2498
                     /gene="cls"
                     /locus_tag="MIW95_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBY3152479.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            2691..2975
                     /locus_tag="MIW95_003175"
     CDS             2691..2975
                     /locus_tag="MIW95_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBY3152480.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            3008..4636
                     /gene="groL"
                     /locus_tag="MIW95_003176"
     CDS             3008..4636
                     /gene="groL"
                     /locus_tag="MIW95_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBY3152481.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVIVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            4898..5638
                     /gene="larB"
                     /locus_tag="MIW95_003177"
     CDS             4898..5638
                     /gene="larB"
                     /locus_tag="MIW95_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBY3152482.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            5722..6456
                     /gene="larC"
                     /locus_tag="MIW95_003178"
     CDS             5722..6456
                     /gene="larC"
                     /locus_tag="MIW95_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBY3152483.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            6486..6905
                     /locus_tag="MIW95_003179"
     CDS             6486..6905
                     /locus_tag="MIW95_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBY3152484.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKVTP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      6965..7066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7287..8822
                     /gene="guaA"
                     /locus_tag="MIW95_003180"
     CDS             7287..8822
                     /gene="guaA"
                     /locus_tag="MIW95_003180"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBY3152485.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTSITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(8899..9945)
                     /locus_tag="MIW95_003181"
     CDS             complement(8899..9945)
                     /locus_tag="MIW95_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBY3152486.1"
                     /translation="MPAYKDERRKSWYASFYFTDFVGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRKWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGTIGKKNAD
                     EMNFWTLEEFKKFIEFEKKPEPKLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IKLNGEDIVSSPKTSKSKRVVPIPCFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LNRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(10021..10236)
                     /locus_tag="MIW95_003182"
     CDS             complement(10021..10236)
                     /locus_tag="MIW95_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3152487.1"
                     /translation="MLKKLRKKKRMTQLELAEKMGRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKFQEIKRRGK"
BASE COUNT         4152 a         1099 c         1944 g         3372 t
ORIGIN      
        1 ataaattttg aaattactta tctttgtgtt aaaataagta aaatacacat attaaagtta
       61 agtttttagg caaataaaca aaatgaaaat ataaatatta aggagatttg gtatatgaat
      121 gtttatgaaa aaggtaatgg agttatacta gaaggagtaa cagattttga cccagtacat
      181 atatttgagt gtggtcaatg ttttagatgg cacaaacaag aagatggctc atacactgga
      241 gttgcaaggg ggagaatatt aaatgtcaaa aaagagaatg ataaaatata tttaaataat
      301 acgaatttaa aggagtttaa cagcatttgg tataattact ttgatttagg aactgattat
      361 acagaaataa agaataagct aaaaaatatg gatgaatatt tgaataaggc tactgaattt
      421 ggatggggaa taagaatatt aagacaagat ggatgggaaa tgcttatatc atttataatt
      481 tcctctaata atagaattcc tatgatacaa agagcgatag aaaatttatc aagaaagttt
      541 ggtaagtata taggtgaata tgaaggtaat gaatactatg cattcccaac accagaagaa
      601 ctaaataagg cctcacaaga ggaaataaga gcatgtcaaa caggttttag agataagtac
      661 ataaaaagta ctacacaggc agttatagag aataatgatg aggtatctga atatacaaat
      721 ttaagtacag aggattgtag aaaagagctt ttaaaattta atggagtagg accaaaagta
      781 tgtgattgta ttgcattatt tggtatgcaa aaatatgatt cttttccagt tgatgtttgg
      841 gttaagagag ttatgcaaga attttatata gatgaagaca tgagtttacc taaaatgaga
      901 acttatggaa tcgataaatt taaagaaatg tctggatttg ctcaacagta cttattttac
      961 tatgcaagag aactcggtat aggaaaataa attaaggggg agaaattatg ggagtaatag
     1021 gaacaatatt tctgttctat ttaattatat catatctagc tggagccata atttctgtga
     1081 ttatattgct tgaaaataga gaccctgcaa aaacaatgtc ttggctattg atgtttatta
     1141 tatttccagg tgtaggttta atgatatatg ctatttcagg tagaaatatt agaaaaagaa
     1201 aattatttaa gactcaaaaa ctagctaata atataaaaga aaagaaacta tttgatacat
     1261 tagaaaagat aacagaaata gttgaattag aaaaagaatc tataaaacaa aataagcttt
     1321 taagagatga agaggatggt agttatagaa aaagggtaat aaatatgttg ttaaagactg
     1381 gtatgtttcc ctttactaaa aataataaag tggatgtatt tgtagatgga aatgaaaagt
     1441 ttaaaagact tatagaagat ataagagaag ctaaagacca tattcattta gagtacttta
     1501 tcatcaaaga tagtgaaata ggtagggtgt taaaagaaga acttataaaa aaggctaaag
     1561 aaggcattaa gataagaatt ttatatgatg atgtaggatg ctggagattt tggtttaata
     1621 gaaaattttt ccgagaaatg agggaagtag gtatagaaat tgcagcattt ttacctacaa
     1681 aatttcctat aataggaggg aaattaaact atagaaatca cagaaaaatt gttgttattg
     1741 atggaataat tggatataca ggtggaataa atataggaga tgaatattta ggcaaaaatg
     1801 ataagtttgg atattggaga gatactcata ttaggattaa gggtatttct gtatacatgc
     1861 ttcaaatgac atttctgatt gattggtatt atacgacaaa agaagtatta gtgactaaaa
     1921 attatttccc aagtgtaggg aatgttggtg aaagtatgat acaagttgtt gcgagtggtc
     1981 cagacagtga ctgggaagat attcattatg cttatttttc tgcaatatgt caggctagaa
     2041 aaaatgttta tatagaaact ccatatttta ttccagatga aagtctacta aaagctataa
     2101 agagtgctgc tcttagtgga gttgatgtga gaataatatt tcctaaaatt gcagaccaca
     2161 agatagtaaa tatagcatct tattcatatt ttgaagaaat attaagagca ggtggtaaag
     2221 tttatttata taataaagga tttatacatt ctaaagtcgt aataatagat gataaaattg
     2281 cttctgcagg tacagctaat atggatttaa gaagttttat gcttaacttt gaggtaaatg
     2341 catttatcta tgatgaagaa gttattaggg taatgacaga tgatttcttt gaggatttaa
     2401 gtcactgtga agaacttaat ttggaggtat ttaagaatag aaatatcata caaaaaatca
     2461 aagaatctgt agcaagatta ttttcaccaa tattataaaa aattattaaa aaaggtattg
     2521 aaaattatgt gtgattggta tattataata attgttagca ctctattaga tagagtgata
     2581 ataaataaaa agtatatata taaatctaca tatataagta gtattaattt aaaaataact
     2641 aaaaacatag gcgaactagt ctatacttaa tattttagga ggcgtgaata atgaaaataa
     2701 gaccattagc tgacagagta gtaattaaaa aagtagaagc agaagaaaaa actgcaagtg
     2761 gaatagtttt accaggagca gctaaagagc aacctcaaat agctgaagtt gtagaagttg
     2821 gaccaggtgg aatagttgaa gggaaagaaa taaaaatgga attgacagtg ggagataagg
     2881 ttatattcca aaaatattct ggaacagaag ttaagataga aggacaagaa tacacaatac
     2941 taagacagag tgatgtatta gctgttattg aataaatata gaataaattt attaggaggg
     3001 atttaaaatg gctaaagaaa ttaaattttc agaggaaaca agaagagctt tagaagctgg
     3061 tgtaaataaa ttagcagata cagtaaaagt aacattagga cctaaaggaa gaaatgttat
     3121 attagataaa aaatttggtt ctccacttat aactaatgat ggagtaacta tagcaaaaga
     3181 gatagaacta gaagatagat ttgaaaatat gggtgctcaa ttagttaaag aagtagcaac
     3241 taaaactaat gatgtagctg gagatggtac tacaactgct acagttttag cacaagctat
     3301 aataagagaa ggtttaaaga atgtaacagc aggggctaac ccaatacttt taagaaaagg
     3361 aatacaaaaa gcagtaatag tagcggtaga agaattaaaa aatcaatcaa gaatagtaga
     3421 aacacaagag gctatatctc aagttgcttc tatatctgct ggtgatgaag aagttggaaa
     3481 attaatagca gaagctatgg aaatagtagg taaagatgga gttataactg ttgaagagtc
     3541 tcaaactatg aatactgaat tagatgctgt tgaaggtatg cagtttgata gaggatttgt
     3601 ttctgcatat atggttacag atgtagataa aatggaagca gttttaaatg acccatatat
     3661 attaattact gataagaaaa tatctaacat acaagaatta ttaccagttc ttgaacaaat
     3721 agttcaacaa ggtaaaaagt tattgataat agctgaagat gtagaaggtg aagcattatc
     3781 tacattagta gtaaataaat taagaggaac atttgatgta gtagcagtta aagctccagg
     3841 ctttggagat agaagaaaag aaatgcttca agatatagca atacttacag gtgctcaagt
     3901 aatatcagaa gaattaggtt atgatttaaa agaagctgat ttatctatgc taggtagagc
     3961 ttcatctgtt aaggtaacta aagaaagtac tacaatagta gatggttctg gagataaaaa
     4021 agctatagaa gatagagtaa ctcaaattaa acatcaagta gagcaaacta cttcagattt
     4081 tgatagagag aaattaatgg aaagattagc taaacttgct ggaggagtag ctgttgtaaa
     4141 agttggagct gctacagaag tagagttaaa agaaagaaaa ttaagaatag aagatgctct
     4201 taatgcaact agagcagctg tagaagaagg aatagtagct ggtggaggaa ctgcttttgt
     4261 tagtgttata ccagcaatag gaacactaat tgagagttta gaaggagaag ttaaactagg
     4321 tgctcaaata gttaaaaaag cgttagaaga gccattaaga caaatagcta taaatgctgg
     4381 tcttgaaggt gctgtaattg tgcaaaatgt tgttaattct gaagcagaaa ctggatttga
     4441 cgcattgaat gaaaaatatg taaatatgat agaagctggt atagttgacc caactaaagt
     4501 tagtagaagt gctttacaaa atgctgcatc aatagcaagt actttcttaa ctactgaagc
     4561 tgctgttgct gaccttcctg aaaaagaaga tgcaggaatg ccaggaatgg gtggcggaat
     4621 gcctggtatg atgtaattta aaaaatttaa aatataaatt ttaatataaa gaactatttc
     4681 gacttagatt ttaaactcta agttgaaata gttcttttgt atatatctaa tttttgtata
     4741 tctataaatt caatacacat atttagataa tcagtatcta gtttgtaata atattttaga
     4801 gtagtattgt tattttttag gttatgattt tagatatata ttaattaagg tataattgtt
     4861 gatagagaat aaatcaaatt taaaaggatg cgataatatg gacttaagaa aattacttga
     4921 agaagttaaa aatgaaaata ttgatataga ttttgcgtta gaaaagttaa aagatttacc
     4981 ttatgaagac cttggatatg caaatataga ccatcataga gaattaagaa atgggtatcc
     5041 ggaagttatt tattgtgaag gtaagagtga tgagcatatt ttagggatta ttgataaaat
     5101 gaatcaaaaa agttctaaca tattggggac aaggtgcaga aaagaaactt ttctgaaaat
     5161 taaagaaatc tataatcatg ctgagtacga agaggcgtct aaaattctta agattcaaaa
     5221 tcatgatata gagaatatag gaaaaggtaa aattgtgatt gctacagggg gaacttctga
     5281 tataccagta gcagacgaag cttatcatac agctaagttt ttaggtaatg atgttgatag
     5341 aatatatgat gttggagtgg caggtataca taggctttta aataaaagac ataaaataga
     5401 tagtgctaga gtaatagtag ccgttgcagg tatggaaggt gctcttgcaa gtgtaattgg
     5461 tggactggtt gatgttccag taatagctgt tccaacttct gttggatatg gagcaaattt
     5521 tggaggatta gcagcattac tagctatgtt aaatagctgt gcatctggaa tatctgttgt
     5581 aaatattgat aatggatttg gagcaggata tttagcatca atgataaata aactgtaaat
     5641 acattgatat aaattaaaca aaactatata ataaaattta atttctatta tatttttact
     5701 attaaaattg aggagacata tatgagtgaa aaaatacttt attttgatat aataagcggt
     5761 atatcagggg atatgaccct tgcaagttta ttaaatttag gtgtgcctaa ggaaattttt
     5821 ttggaggaaa ttcataaact aagaatgagc gatgaattta atataaacat cagctcgaaa
     5881 actgaaaatg gaatagtagg tacaaaagta gaagtaatta caaaagaaaa acatacacat
     5941 agaaatttag tggatatatt tgaggttata gataaaagca ctctaaacga aaatgtaaaa
     6001 tctaaagcaa aaaaaatatt tatggtaatt ggagaagcag aagcaaaagt tcatggaact
     6061 acaatagaca agatacattt tcatgaggta ggggctatag attcaattgt agatatagta
     6121 ggagcttcta tattagttga cttactttgt gtagataaag tttgtgctag tacagtgtct
     6181 gttggttcag gatttgtaaa atgtgaacat ggaattatac ctatacctgc acctgcaact
     6241 atagaaattt taaaaggtgt gccaattaaa ttaaataatg taaatggaga atgtacaact
     6301 ccaacaggag ctgcaatcat aaaagtcttg tgtgatgagt ttgtagatga gcttgaattt
     6361 tctccaaagc aaattggata tggtataggt cataagaagt ttgaagtacc aaatatactc
     6421 agaactgtat taggtgaaaa aaaaaagaag aattaattta tgaaattagt gcaaacattg
     6481 atgatatgtc ttcagaaatc tattcttatt tgtatgaaaa aatattagat gagaaagcat
     6541 tggatattta tacagagagt atttatatga agaagaatag accagcaaca aaagtatcaa
     6601 ttttatgtaa tgaatcagat ttagaaaagt ttattaaaat attactttta gaaactagta
     6661 cttttggagt gagatataat gcatataata gagaaatttt agagagaaaa tttgtaaaat
     6721 tagatacaaa atatggagtg gttacagtaa aactagggta ttataaagga aaactagtca
     6781 aggttactcc agaatataat gaatgtaaaa ctatagcaaa aaatgaagat attcctataa
     6841 aaaaagtgtt tgctgatata aattttagaa taaaagaaga atttagtgaa aacttattga
     6901 cataagtaat aaattttgat aaattaatat tcagccttat taaataaaag taaacagaaa
     6961 aaaatatata aaatataaac tcactcgtat atgctcagaa atatggtctg agagtctcta
     7021 ccaagatacc gtaaatatct tgactatgag tgaaattatt ataccaaaag acctagttaa
     7081 gtaaatgtgt aggctgagtt atactcccta tgtgttggct ataagataaa ggtattttgg
     7141 aagcctgtat agtaatttca cttttgctag aaattatgga agttactata caggtttttt
     7201 gtgtaaaaag atttaatata atttagactg tattgtgtta aaataaatat ataaaaacta
     7261 tattatacaa ggagagatgt gaatttatga aacatgaatt agtacttgta atagattttg
     7321 gaggacaata taatcagttg atagctagaa gagttagaga aaacaacgta tattgtgaaa
     7381 tacttccatg tactgcaagc atagaaagaa taaaagaaaa aaatcctaaa gggataatat
     7441 ttacaggcgg tccaaatagt gcgtatttag aagattctcc aacaatatca aaagaaatat
     7501 ttgaactagg agtaccaata cttggaatat gctatggtat tcaaataatg tctcatgtat
     7561 tgggtggagt tgtaagaaaa ggtaataaac aagaaaagga atatggaaaa acatctataa
     7621 cttatggaaa atcatcatta tttgaaggaa taactacaaa tagtgtatgg atgagtcata
     7681 cagatttaat agaaaaagtt ccagaaggat ttactatagt tgcaaatact aatgattgtc
     7741 cagtagcagc tatggaaaat gtagaacgaa acctatatgg agttcaattc catccagagg
     7801 tagaacactg tttggaaggg gacaagatac ttacaaactt cttatataat atttgtaaag
     7861 taaaaggtga ctggactaca gattcattca tagaagataa aataaaagaa ttaaaagaaa
     7921 aaataggcga taaaaaagct ttatgtgctt taagtggagg agttgattct tctgttgctg
     7981 cagttcttat tcataaagca attggggaca acttaacttg tatatttgtt gaccatggtt
     8041 tacttagaaa aaatgaagga aatgatgtag aaagaatatt tagagagaaa tttgatataa
     8101 atttaataag agttaatgca gaagatagat tcttatctaa attaaaagga gtttccgaac
     8161 cagaagctaa gagaaaaata ataggtgaag aatttataag agtatttgaa gaagaatcaa
     8221 ataaattagg aaaaatggat ttcttagtac aaggaacaat ctatccagat gttatagaaa
     8281 gtggacatgg aaatgctgct actataaagt ctcatcataa tgttggtgga atcccagaag
     8341 atgtagactt ccaagaaatt gtagaaccat taagagagtt atttaaggat gaagttagaa
     8401 aaataggatt agagcttggt atagaagaag gattaatatt tagacatcct ttcccaggac
     8461 caggtctagg aataagagtt ataggtgatg taactaaaga aaaatgtgat atattaagag
     8521 aagctgatgc tgtttatatg gacgagctta gaaaagcagg tttatacaga gaaatatggc
     8581 aagcttttgc aacattacca gatgttaaga ctgttggagt catgggtgat gaaagaacat
     8641 atgcttattt agttggttta agagcagtta cttcttctga tggaatgact tctgactggt
     8701 ataagatgcc ttatgatgta ttagaaagaa tttctaatag aataataaat gaagttgatg
     8761 gagtaaatag agtagtttat gatataactt ctaaacctcc tggaactatt gaatgggaat
     8821 agtaaacaaa aaagtcgtaa gctttgtgat ttcaaggctt gcgacttttt taaaaatgct
     8881 tatgatacta aaatgatact atatatttaa attatctagt ttttgagcaa cttcaagttg
     8941 cttattcggg tataaatgcg aatatgtatt ccaagttgtt tctacttttt catgccccaa
     9001 tctttccgct atagttaata tatttacatc catattcact aataacgacg catgagaatg
     9061 tcttaaatca tgtactctaa ttctttttac attagataac ttacaacatc tatttagttc
     9121 tttagaaaga taacttttag caaatttaaa tattctttca ttatctttta agtcatacag
     9181 cttagataga taatctttta tattattata taaaaaacat gggataggga caacacgttt
     9241 acttttagaa gttttaggag aagaaacaat atcctcacca tttaatttta tgtaactttt
     9301 ttcaacactt attttatttt caaaaatatc ttttggagta agagctaata actcccctag
     9361 tctaagacct gtccaaaata aaatttcaaa agctaattta ggttctggtt tcttttcaaa
     9421 ttcaataaat tttttgaact cttctaatgt ccaaaaattc atttcatctg catttttttt
     9481 gcctattgta cctgctaaat gagcaggatt agaaggtaag ttataatatt taactgcata
     9541 atttaataca gcaacaagct ggttgtttat cgtttttata tatgtttgac tgtaatcact
     9601 ttttaataat tcattttgcc atttccgtat atgtgtggca gtaatttcat ttatttttaa
     9661 acttttaaaa aaaggtaaaa ttctaaagtt tattaaatac tttttagttt ctaatgtaga
     9721 taactttaat ctagaagaca tatcgtgcat gtattcttct atgagacttt caaaactcat
     9781 atcagtactc attttagatt tatttaaaaa ttctctttca aactctagag cttctttttt
     9841 agttttaaaa cctctcttaa ttttcttttt cctatcccca acgaagtctg taaaataaaa
     9901 gctagcatac caactttttc ttcgttcgtc tttgtaagca ggcattttag acacctccaa
     9961 ttatgtcatt ttatattaca ataattataa catgttagat atgctatcta aataattgtt
    10021 ttattttcct cttcttttta tttcttgaaa cttgtaatat ttacacagtt ctaagaagtc
    10081 ttcttctagt gctatagata agtctaatat cgtagatata cctatatctt tgtactcttg
    10141 attttccagc tttgatatat aacttctgtt acgtcccatt ttttctgcta attctagttg
    10201 tgtcattctt ttcttttttc ttaatttttt caacatttat taaaatccac cttttaatta
    10261 tttttatgac atttttataa ttaatagttt gtgtaatttc ttgtaaaaaa tgttcctcgt
    10321 tagaacattt tttggtggga aaatgtgtta aaatgtaagt aagaaaagca cattaaaagc
    10381 actataagaa tagtgctgag atgctagagt ttaaagaaag aataaatttt atttaaaagc
    10441 ttataaatat aaagtaaata atttgtaaat taaatctaag aataatgtaa aaaatgcaat
    10501 aaaaaaataa aaattaaaat tatgaaaaat aaagctagcc gactaactaa aaatagaaat
    10561 atacaaa
//
LOCUS       DAGQWR010000143        10416 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.144, whole genome shotgun sequence.
ACCESSION   DAGQWR010000143 DAGQWR010000000
VERSION     DAGQWR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.144"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            244..1668
                     /locus_tag="MIW95_003183"
     CDS             244..1668
                     /locus_tag="MIW95_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBY3152488.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDALFKRLESK"
     gene            1703..3832
                     /locus_tag="MIW95_003184"
     CDS             1703..3832
                     /locus_tag="MIW95_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBY3152489.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDK"
     gene            3862..4284
                     /locus_tag="MIW95_003185"
     CDS             3862..4284
                     /locus_tag="MIW95_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY3152490.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4303..5454
                     /locus_tag="MIW95_003186"
     CDS             4303..5454
                     /locus_tag="MIW95_003186"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBY3152491.1"
                     /translation="MKKAIQFGAGNIGRGFIGSLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEEILNNIEKSYIFMKNL"
     regulatory      5560..5661
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5844..6416
                     /locus_tag="MIW95_003187"
     CDS             5844..6416
                     /locus_tag="MIW95_003187"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBY3152492.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6480..7130
                     /gene="fsa"
                     /locus_tag="MIW95_003188"
     CDS             6480..7130
                     /gene="fsa"
                     /locus_tag="MIW95_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBY3152493.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGAFLKL"
     gene            7282..10206
                     /locus_tag="MIW95_003189"
     CDS             7282..10206
                     /locus_tag="MIW95_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3152494.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYGTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4272 a         1010 c         1950 g         3184 t
ORIGIN      
        1 attattattt tcatttctat taaatttaaa tattttataa aaatagtatt atttacaaaa
       61 tcatatactc caaaatacta cataaataaa attaaacaaa aactaaaatg acaaatgtca
      121 ttatctaaag tgacatagaa acggtttctt aaagttgtta ctataaaata taaactctat
      181 aataaaagta taacaaaagg gaatcatatc gatatagaat ataattcaag aggaggtaaa
      241 attatgagta gttctaatca atcgacaata aagacatcta tacagcgagt agggaaattt
      301 ttaagtggta tggtaatgcc aaatatagga gcttttatag catggggtct tataacagca
      361 ttatttatac caacaggatg gatgcctaat gaaaaattga gtactatagg cgacccaatg
      421 ataaaatacc ttttaccact attgatagct tatactggtg gtaaagcaat agcaggacaa
      481 agaggtggcg taataggtgc agcagcagct atgggagtaa ttgttggtgc agatatacca
      541 atgtttatcg gagcaatgat aatgggacca tttgcaggat gggtaattaa gaagtttgat
      601 aaatttgtag atggaaagat accaacagga tttgaaatgt tagtaaataa cttttcaata
      661 ggtataatag gtatgcttct tgcaatacta ggattttatg caattggacc agcaatagta
      721 gcagggacag ctcttataga atctggagtt caatttatag ttagcaaaag tttattgcct
      781 ctagtttcag tatttataga acctggaaaa gtattattct taaacaatgc aataaatcat
      841 ggtatattag ggcctatagg tatagctgag gcaaaagaag caggtaaatc tataatgttc
      901 ttactagaaa gtaaccctgg accaggttta ggagttctat tagcatattg gatgtttagt
      961 agaggaagtg taaaacaatc agcaccagga gctgtaataa tacatttctt tggtggtatc
     1021 catgaaattt acttcccata tattttaatg aatccagttt taatattggc tacaatggca
     1081 ggtggagcag caggaatatt gactttttca atattaggag ctgggcttgt agcagcacca
     1141 tcaccaggaa gtatatttgc acttatggca ctagctccaa agggaggatt acttccagta
     1201 ttagcaggag ttgcagtggc aacagttgta tcattcttag tggcagcacc atttgttaag
     1261 agagcttcag caaatcaaag tgaagaagac agtacttctt tagaagaagc taaagcaaaa
     1321 atgagtgata tgaaatctgc aagtaagaac tctgagaaaa atattgagga aaaacagctt
     1381 gaagtaaatg agattaagaa gattgtattt gcatgtgatg caggaatggg ttcgagtgca
     1441 atgggagctt ccagatttaa aaatagaata aaaaacttag acttaaatat agaaattaca
     1501 aattcatctg ttgataatct tccagatgat acacaaatag tagtaactca caatacatta
     1561 gttgaaagag ttgctaagaa taattcaagt gttgagatag tttcaataaa caatttttta
     1621 aatgacccaa atttagatgc attatttaaa agattagaat ctaaataaaa ataaaaagta
     1681 ccactaatta ggagtggata atttgaaaaa aaagaagatt acatctaggc aaaaaaagat
     1741 aatactaatg atagtagaaa attcaaagaa aaacattcca ataactattt ctgagattgc
     1801 agggacttta gagctaagtt caagaactgt acttagagat atgtctggaa ttgaaaaatg
     1861 gtttgatgaa aatgatttta actttgtaaa aaaaccagga gtgggattga ttttagagga
     1921 aaatatagaa aatcaaaact ttataattga gttgttagaa gaagaaaaaa tagaaaaaga
     1981 atacagcaag gaagaaagaa atttaattat attatcaaag ctactagttt ctaacgagcc
     2041 agtaaaatcc tattattttg caaaaatatt aaaggtatct gaaggggtac tgaataatga
     2101 ttttgcctta gcaagtaaat ggttggaaag atttgatatt gagcttgtta gaaaaccagg
     2161 gcttggtgtc tacttaaaag gtcaagaaaa aaactttaga gaggcatatg taaatcttat
     2221 atacgattca ttcaatgaaa aagaaatact agatatggtc agaaatataa gtgaaaatat
     2281 acaaacagat aaagcaatag aaattttaag tgaaaataga cttttaaatt taatggacag
     2341 gtgcataata agaaaagttg aagctacatt aacaaagaag ctttcagatt tagatgtaaa
     2401 tttagctgat agtgcatata tagggcttgt agttcatatt tcattagcct tacagaggat
     2461 aaaaaatggt gaaaatataa ctatggataa agagtttcta aaagaattat ctataacaga
     2521 agagtttaaa ttagctaaag aaatcgtaaa aggtatggaa acagatttta acatggatat
     2581 accagtagat gaggttggat atataactat gcatataaga ggtgcaaagc aaaggtcatc
     2641 ttcaaaccat aaggctttaa acttagatga tatagaaata atggaaatta caaataagat
     2701 gatagatttg gcggaagatg aatttaaaat aagtttaaaa aatgacgaga ggttatttaa
     2761 agacctagca aatcacttag ggccttctat aaatagattg aatatgggat tagaaataag
     2821 aaatcctctt ttagatgaaa taaaaagcaa gtattcatat gcctatgatg gagttgaaaa
     2881 gatttctaga attataaaag ataaactaaa tataaattca atacctgaat cagaaatagg
     2941 atatattgca atgcactttg catcagccat agaaaaaaat cttatgatga atactaatat
     3001 aaatattgtt gtggcatgtc ccactggaat aggtacttca agattcttat caactaagat
     3061 tgaaaataaa tttcctaatt taaatatact tgaaaccata tcagctataa acatagatga
     3121 agagtattta aaagaaaaag atgttgattt aatagtgtct acagttgagt taaatacaag
     3181 tttaaattat atatgtgtag gtccatttat gagtttagat gatgaacaga taatcaagga
     3241 aaaaataaaa tctatagctc aaaataaatt aatcaattta aatgcgaaaa atgatactaa
     3301 aagtaagaat aaagtatatg aacagataac agaaagtatg aatataggaa aagatattct
     3361 tcaattttta gaggaaatta gatttgaaaa atttgaaagt aaagatttaa gtgagcttat
     3421 tgaagactca tcaaaaatat ttgcaaaatc tagtgaagat ataatctcta taaaagaatc
     3481 gttaaaagaa agattaaaaa tctcaattcc atatatagaa gaatcaaaaa ttctattgtt
     3541 acattgtatg agtgaaagaa tagatattat gaagttatca ataataaaac tagagaatag
     3601 aatcgtgcta gattcaaatg aagaaatcga taatgtagtt tttatgttac ttcctaaaaa
     3661 ttcaccaagt tatcaaagac aaataatgag tgaaataagt ggttcattaa tagacaatat
     3721 aatttttact aataagataa ataaattctc tatagaagag atgacattag aggtaaaaga
     3781 tatagttttt aatttttata caaacagatt aaaagcattt atagataaat aaaattaata
     3841 ttaaaaagga aggttgatac tatgagtaaa gtattgaatg agaataatat atttttaggg
     3901 ttagatagtg tttcaaagga agaagctata actttagcag gaagaaaatt agttgaaaat
     3961 ggatatgtaa aagaagaata tataccagca atgttagaaa gagaaaaagt aatgactaca
     4021 tatatgggaa tgggagttgc tattccacat ggtgtaaatg aagcaaaaaa agaaatatta
     4081 tcttctggaa tagttatatt acaattccct aatggtatag attttgatgg agaaaaagca
     4141 tatttattaa taggaatagc tggtgttgga gatgaacatt tagaaatact atctaatata
     4201 gcagtagttt tagatgatga cttaacagaa agactaaaaa attcaaatga caaacaagct
     4261 tttatggaag cttttgccaa ctaatcttag gagggattta aaatgaaaaa ggcgattcag
     4321 tttggagcag gaaatattgg aagaggtttt attggaagct tgttagttaa gtcgggttat
     4381 catgtagtat ttgctgatgt aaatgaggag atattaaact caataaacaa agataaaaag
     4441 tatactatac acataagaga tgtagaatgt atagatgaag taattgataa tataagtgcg
     4501 gtttcatcta taaaagaaga aataatagat gagattgtac aggctgaaat aataactaca
     4561 gcagttggac ctttagtttt gactaaaata gcctctacaa ttgcaaaagg tataaaagct
     4621 agaaaagaaa agggattaac aagtaatcta aatataatag catgtgaaaa tgcaatatac
     4681 gcaagttctt ctttaaaaga agaggtactt aagtatttaa ataaggaaga agtagaatat
     4741 ttagagatgt atgttggttt tccaaactgt tcagttgata gaatagttcc tcctggtaaa
     4801 aacgaaaatc ctcttgatgt aacagttgaa aatttttatg agtggaatgt tgaaaaacaa
     4861 ggatttaaag gggaaatacc aactatagtt ggaatgaatt tagctgataa ccttatggct
     4921 tatatagaac gtaaattgtt tacactgaat acaggtcatg ctataacagc ttatatagga
     4981 tatttaaaag gatataagac tatagaagag agtataaaag ataaatttat ctgtgatata
     5041 gtcaaaagtg caatggtaga aagtggagaa ggattgatta aaaaatacaa ttttgactca
     5101 gaagtgcact ataaatatat tgataaaatt ttaaatagat ttaaaaatcc atatcttaat
     5161 gatgatgtat taagagtcgg aagggaacca ttaagaaaac taagtgataa agatagattg
     5221 attaaacctt taatgacagc aaaatcatat ggattatcag ttgacaattt gattcttgga
     5281 ataggtgcag cgcttcatta taataatagt gaagatactc aaagtgtaga attacaagag
     5341 ttgataaagt ctattggggt aaaaaaagcc gttgctaaaa tagctaatat aagcaatgac
     5401 gaagaaattt taaataatat cgaaaaaagt tatatattta tgaaaaattt ataaatttac
     5461 ttgaaaaaaa gtagttctga taaagtaact ataatatatt attctacaca gtttgaatta
     5521 tttgttgaat aatagagaat tataatatat aattaaaagg aataaattaa tatatttacc
     5581 tgtatatgtt tgataatatg gttcaaatgt ttctactaag ctaccattaa tagcttgact
     5641 acaggagtga agaagatata gtttatactt attttaaatt atatattaag ttaattactt
     5701 tgaattttaa gctcctgtct atgatataga caggagcttt tttagtattt taatataaaa
     5761 tacttacaag aatagatagc aaaattagaa caaatataat ttttttatta atataatttt
     5821 agaaagggct aggtggaagt tcaatgaagg ctttacaaga aaagatattg agagaaggca
     5881 gtgtttcagg aaatgatata ctaaaagttg atagtttttt aaaccatcaa attgatgtag
     5941 cttttttaaa tgaaatagga agagaattta aagagagatt taaaggagaa aaggtggata
     6001 agatatttac aatagaggct tcaggaatag ctatagcatc aattgtttca cagtattttg
     6061 ataatgcacc tgtagtattt gcaaaaaaat cagaatctaa aaatttggat aaagatgtat
     6121 atgaaactaa tgtatattca tttactaagg caagagagta ttcagttaaa gtttctaaaa
     6181 agtacataaa caaaggcgaa aatatattga tagtggatga ttttttggca aatggaagag
     6241 ctgctcttgg acttaaagat ttaattgagc aagcagaagc taacttagtt ggagtaggaa
     6301 ttgtcattga aaaaggattt caagctggag gagcactttt aaaggctaat gatgtaagat
     6361 tagagtcttt ggcagtagtt gaatctatag ataatggaac tgtaaaattt aggtaattta
     6421 tgatattata taattgtgtt tagcacatat tttaactatg taattatgga ggatttttta
     6481 tgaggttttt tatagacaca gccaatattg aagaaataaa agaagcaaat gatttaggtg
     6541 taatatgtgg tgtaactact aatccgtctc taatagctaa agaaggtaga gattttatag
     6601 aggtagttaa agaaataagt gaaatagtag atgggccaat aagcgcagaa gtaataagtt
     6661 tagaacataa aggtatgata gaggaagcag aaaaattatc taagatacat aaaaatatag
     6721 ttataaaaat acctatgaca gcagaaggtc taaaagcggt gaaagtacta tcaagtaaag
     6781 gtataaagac taatgtaacg cttatatttt ctgctggaca agctctttta gctgctagag
     6841 ctggagctac atatgtaagt ccttttgttg gtagattgga tgatatatca caaaatggtc
     6901 ttgatttaat tgaagaaatc gttgatatat ttagtgtaaa tagtattgag gctcaaataa
     6961 tagttgctag tgttagaaat ccaatacatg ttttacaagc tgctagaatg ggagcagata
     7021 tagcaacagt tcctcttaaa gttataaacc aaatgattaa gcatcctttg acagacaaag
     7081 gaatagatag ctttatgaag gattgggaag gtgctttttt aaaattataa ttataaacta
     7141 cgctttataa gtatattaaa gaggttatta tggaaattct aagtaataag gctaggctat
     7201 tttgcctagt cttattttgg tgtataatat attgaaacat attattgaaa tagattaatt
     7261 ttttaagtga ggaggcgaaa aatggataaa atattaagag agatacaaag agaagataaa
     7321 aaaaatccat atactgacca agaacttgct gaaatgttaa atgtagctag gtctgaagtg
     7381 atatcagtta ggaaaaaaaa taatatttta gattcgagag aaagaagaaa aaaaatatta
     7441 ataaaagata tatcaaaaat tgtaagtgaa aatcctcaaa tgtctgaaag aaaaataact
     7501 gaaatattaa tagaaaatgg atataatata tcgagaagtg cagtttcaaa attattaaaa
     7561 gaggaaaatt taaatcagtc aaagattggg attaaggata aagacccagc atcatcagga
     7621 aagtatgtga aggtgagtaa agttgtagaa gaagatggat ttgaagaatt gataggtata
     7681 aagggaagtt tgaaagaaaa ggtaaatcta gctaagtcag caataatgta tcctcctaat
     7741 ggccttcata ctataatata tggagaaaca ggagtaggta aaagtgaatt agctacatgt
     7801 atgtataaat atgctataaa aaacaacatt aaagaagaga acagcccatt tatagtgttt
     7861 aactgtgcag actatgcaga aaatcctaat ctattgatag ctcaactctt tggggtagta
     7921 aaaggtgcat atacaggagc agatgcaaat agagaaggtc ttgtagaaca agctaataat
     7981 ggaatattgt ttttagatga aattcataga ttgcctgctg ctgggcaaga aatcttattt
     8041 tcattaatag atagaggaga atttagaagg ttaggagaaa gtagttctaa tagaaaggca
     8101 aatgttctca taataagtgc tacaacagaa aatccagatt ctaatttact tcaaacattt
     8161 agaagacgta taccaatggt aataaattta cctaatatga gtgaaagacc aaaatctgaa
     8221 agatatgaaa taataataaa gttttttgaa agagaagcaa agcgagtaaa caagaatttt
     8281 ataataacta aagaagttat gtgtgcactt atgaattata agtgtactgg aaatataggt
     8341 cagttaaaga gtgatataca agtaacttgt gctagagctt ttagtaaatc gatattttct
     8401 agcgataagg taattataga tttagatagt ttaagggatt atataaaaag tggatattat
     8461 gattgtaatt gtaaaaatga aaagtattct gagttttcag ttgaggatat atatatagat
     8521 attagtgaat tggtgagtgg aaaaaataga attttatctg ataatgagat aagcgagata
     8581 tataattatg cagaaaaaga gcttaaggtt ttggaaagta agtgcttttc agaagaagag
     8641 ttaaaaaatg catttataga aaagttagat aagaagttta atgctattaa aaacaataaa
     8701 aatttatctg atagaagaag tcgaatagac tggggaggac aattaaaaga gtctacactg
     8761 gagataatgg ataaggtaat tgtatttatt aaggagcagt ttaagcatgt aaatcaaggt
     8821 ctgtacttgg ctctagcaat tcatattgag catgctatag gtaggataaa agatggaaaa
     8881 actataataa atccgagttt agataaaata aaagcatcaa tgccaatgga atatgagttg
     8941 tcaagatata taatgggtat agtagaagat ttgacacaag taacatttcc agaagatgag
     9001 cttggatatt tggcttatta tataaataaa ttttgttata atgaggaaag tataaaagat
     9061 aaagttaaag tagtaatagt tacgcatggt aaagttggaa ttgaaatgtc taaagttgta
     9121 aatcatattt taggtataga atgtacacta ggtatagaaa tagcacttac agattcacca
     9181 tcagagggga tagagcatgt attagaagag cttcaaaaga tagaagctag aaaaggtatt
     9241 ctagttctaa ttgatatggg ttctttggta attcttggtg atgaggttga aaaaaggctt
     9301 ggaattagat gtaaaactgt aaatagagta gatacattat tggcaatgga agcaggaaaa
     9361 cttgcaacca ttgaaggaaa aagtttagat ggtataatag ctgatttaaa gaaaaataag
     9421 aattatgcta tgataaatac aaataaattt agttacagaa aaaacgagta tggaaagaaa
     9481 aatgtaatca ttactttatg tttgagtggt gtaggtacag ctttaaattt aaaagagcat
     9541 atagaaaaac aaattaagga atatggcact ttaattgaag taaagccagt tgctttttta
     9601 aataataatt tagaagaaga gttaaatgat attgaagctg aatataatat tattgctatc
     9661 tgtggaacta ttgatataga ttataaaaac gtgcctttta tttcttataa tgaggtactt
     9721 ggtaaaaatg gtattaataa agtattagag catctaaata gtaaacaacc agctttaaat
     9781 attgaaaacc agttgaataa tttaatacat gaagatttga ttttatacaa ttttgaagga
     9841 atttcaaagg agtatataat tgatactctt gtttcaaaac ttgaagaagg tggatatgtt
     9901 gattcaaagt atatattaag tgtatataaa agagaagcaa tgggaagtgt tgtaatggcc
     9961 tctaaggtag ccgtgccaca tggacttcca gaaaacgtta taaagcctgc aatagcaatc
    10021 gcaaggttga ataaacctat agtatgggac aataaattta tggtggattt ggtggtacta
    10081 ctggcactta aagaaaataa taagaaagaa atacgaagct tattttcaaa gattaatgac
    10141 cagcatactt tagaagtttt attaaatact gaagataaaa atgaaataaa agaactttta
    10201 agctaattat tagtaccgag aagaaagaca aaaaaatttg ctttagtata aaatttaaaa
    10261 ttactcaatt gctaagtagt taattactct atataaggct agttctatga aatttattta
    10321 tatgatttta tagaattagc ttttttcttg gtaaataaaa tatatgaatt tatttatgta
    10381 aaaagtaaat tttattggac aattctatga aaattt
//
LOCUS       DAGQWR010000144        10402 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.145, whole genome shotgun sequence.
ACCESSION   DAGQWR010000144 DAGQWR010000000
VERSION     DAGQWR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.145"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            246..2435
                     /locus_tag="MIW95_003190"
     CDS             246..2435
                     /locus_tag="MIW95_003190"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBY3152495.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEEFIDIKTDLEKVTKANISIRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
     gene            2503..3270
                     /locus_tag="MIW95_003191"
     CDS             2503..3270
                     /locus_tag="MIW95_003191"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBY3152496.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            3328..4344
                     /locus_tag="MIW95_003192"
     CDS             3328..4344
                     /locus_tag="MIW95_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBY3152497.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVYAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            4410..4658
                     /locus_tag="MIW95_003193"
     CDS             4410..4658
                     /locus_tag="MIW95_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152498.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELGVLLEEKKELEEE"
     gene            complement(4835..5332)
                     /locus_tag="MIW95_003194"
     CDS             complement(4835..5332)
                     /locus_tag="MIW95_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBY3152499.1"
                     /translation="MYKIGESVMYPKEGACYISGLVTKDINHHIQKYYELTVIYNSNL
                     KISIPVLNADKIGVRPIMDENEVENFIQSLDKVDCLWVFDRKKRLKLYHDKFHSGDVF
                     EIVKLIKMLMIQDNSKQLCSTDKDFLNKAQRFALSELAAAQCKSYTIVLEEMKNHILN
                     SKNNN"
     gene            complement(5573..6637)
                     /locus_tag="MIW95_003195"
     CDS             complement(5573..6637)
                     /locus_tag="MIW95_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152500.1"
                     /translation="MIRLTIANIKRYIKNHALLVNMVLFPVILMFYLNFFTGNSENQS
                     MYFSPVAIVSNSSGKYEHKLIDSSKLKENSFGLNEQDKAMDLLKNNKVSAVFILDKNF
                     SSNIDNLKRPEVKCFKTENGGGSLWAESQIESFITNSLKLKIDRNIDDKLTKTNIIDN
                     SSGKNKNSFLIVFLICYFMYINAAHLASDLFSLKKSNTLKRLVSTKHNDIKIIFSIFL
                     GLFFTQAIVYTLTLVCFTSISNFKLTLNTLMIVLANSFVATGFVFWIARVFKNEGSIS
                     LMSTFYSLIGLAISISNMIPSLDKLSFMANLSKLTPFYWTIDAINNNGNILINIIVLI
                     LIGMVFVTAGSLNLRDFARN"
     gene            complement(6640..7737)
                     /locus_tag="MIW95_003196"
     CDS             complement(6640..7737)
                     /locus_tag="MIW95_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152501.1"
                     /translation="MKLYISVKTMLKVLKSSFMLNLIYFLALPLVLAGFLGMVTESIF
                     QNPIKTDLTSIVIYDKDNTKLSSNLTGYLKNDLSSILTVQKDDSKADLKLTIPKGYEN
                     NLLNEKSNNLSIEKLKSNDNIAILLQDILDTYHEKLYLNNSQKLSSEDFSKLFNKNSI
                     DTSIIKNNVKQNSYEYFALVSLGFFAIIFIMNSILSNYVSESKGLSKRLYSIPVTRVQ
                     FLIYDFIGFWIYSFIFLSLYVLFFRTLGVTFKGNFVILALLCALASYFMTSISTFITT
                     FFSKKYGTVIVYALLFLQTVFGGIFSIVNDVFTKFTSLSPTYLIGTLFSNYETFKTID
                     SIESLIFTCLIASTVLIALSIVKEKYKWREV"
     gene            complement(7751..8686)
                     /locus_tag="MIW95_003197"
     CDS             complement(7751..8686)
                     /locus_tag="MIW95_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152502.1"
                     /translation="MDIVRVNNITKRFNDKLALDNISFSVKEGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGDIQINGLDLSKDCIKAKSIIGLVPQELALIETLTPFDNLEYFGT
                     YYGLKGKLLQERITEALEVTGLTDVKKKKIKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKNISKKQKTTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVVSILENNSLLELGVDKKLQLTDV
                     LSVVDKNNSKITRISYEEPSLEDVFLNLTGKNLRD"
     gene            complement(8874..9518)
                     /locus_tag="MIW95_003198"
     CDS             complement(8874..9518)
                     /locus_tag="MIW95_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3152503.1"
                     /translation="MNKIKVLIVDDEKLIRKGLNIILSSYDDLEIVGDASNGYEALEF
                     CKTNDVDIVLMDIRMKVCDGVLGTRLIKEYDSSIALLILTTFNDDEYIEDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMYPQKTIEQKHISDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILGKLELRDRTQLVIFAFKNKIVI"
     gene            complement(9511..>10402)
                     /locus_tag="MIW95_003199"
     CDS             complement(9511..>10402)
                     /locus_tag="MIW95_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY3152504.1"
                     /translation="DASFLIKEKYKYILFSLVIIFSFIIGLDKNLYLALESSSTLLII
                     TLFSVYLENENLSKLYNQSLYDKLRVSKDELKKANADLEIYANSIEELAILKERNRIS
                     REIHDSVGHSLSTTIIQLNAIEKLLKDKPLISDLVHELREFVTESFQDVRRAISELKP
                     IEYENYQNLLKIRELVNSFIKLTNINVRLTISKNTWNLSTNQSIALYRLVQESLSNAS
                     RHGKATEIRIFITFNPSNLIITISDNGIGCNNIKRGNGLSSISERIYELNGKVEFDNS
                     NTGFTIRASFPKFSGGDFFE"
BASE COUNT         3958 a         1225 c         1642 g         3577 t
ORIGIN      
        1 aaaaaataaa gtatataaga gtataatttg acttttatat acttttttat attatttttg
       61 agttttttag gaagatattt tacaagttaa gaaaactata aggagtgtta tattgaatat
      121 aaaatagtat aatatatatt atgttagata attaatatga aaatatacaa atatataaaa
      181 aagtttgttt agagttatag ttaatgtgtg tattaattta atactaattt attggaggtt
      241 taaaaatgaa atacataata aaaagagatg gtacaaaaga actttttagt aaaagtaaga
      301 ttgaagatgc tgtctataaa gctagtataa atagtgaagg tggagtagat agagagttgg
      361 catatgatat atcaagaaaa atagctaatg actataattc tatggaaaaa tgcttatccg
      421 tagaagaaat acaagatata gttgaagtac tacttatgga atcaaataga aaagatatag
      481 caaaacacta catattatat agagaaagac gctctgaaat aaggactaaa gcttgggagt
      541 ttgatgaatt acaaaaatca atatggaaga acaagtatag acataatggt gagagttttg
      601 atgaatggat aaagagaatt tcaaataata gttcaagaat atcaaaacta ataagacaaa
      661 aaagatttct atttgctgga agaattttag caaatagagg attacagaaa gaaggaaaaa
      721 aagtaacata ttctaattgt tatgtaatag aaccaccaaa agatagcata gaagatatat
      781 ttgatactgc aaaaaaactt gctagaactt ttagttatgg tggaggagtt ggaattgata
      841 tttcaaagct aagaccaaaa ggagcgaaag ttaacaatgc tgcaaaacat acgactggag
      901 cagtctcttt tatggaactt tacagtatga caactggctt aattggtcaa agagggagaa
      961 gaggagctct tatgatttct atggatgtaa atcatccgga gatagaagag tttatagata
     1021 taaaaacaga tttggagaag gttactaaag caaatatttc cataagggta acagatgaat
     1081 ttatgcaagc tgtaagggaa aaaaagttat ataattgtac ttttgaagtt gaaactactg
     1141 gagaaaaaat agttaagata gtaaatgctt atgatttatt taataaattt gtgaaaaata
     1201 actgggatta tgcagaacca ggtatattgt tttgggataa aataaaaaca tataatttta
     1261 tgagtgaaga taagaacttt tgcttttcag gagtaaatcc ttgtgcagaa gaaccacttc
     1321 caagtggagg aagttgtcta cttggctcaa taaacttgtc agagtttata atacaaccat
     1381 ttggtgaaca tgctactttt gatattaata aattaaaatc ttgcgttaga gaagctgtag
     1441 tagctttaaa tgatgtctta gatgaggggt tgaacttgca tccattacaa gaacaaagag
     1501 atagtgtaca taagtataga cagataggtt taggtgtaat gggtatagct gatatgctta
     1561 taaaaatggg aataagatat ggttctgatg aatctttaga actatgtgat ttaattggta
     1621 agactatgtt aaatgaagct gtgaagcaat cagcattaat tgctaaagaa tatggaacat
     1681 ttagtgagta taaatatgaa aatatttcta aatctaagtt ttttatagaa aatctaaatg
     1741 aagacactaa agaaatggtt aaaaagtatg ggcttagaaa ttcacaactt ttaaccatac
     1801 ctcctacagg ttctatatct acaatgatag gtatcagtgg tggaatagaa cctatattta
     1861 atctttcata tataagaaaa actgaaagct tgcatgatga ggatgtgtat tataaagtat
     1921 atactccaat tgttaaagag tatatggaca agaaaggtat tacagatgaa gaatgtttac
     1981 cagatttttt tgttactgct atgaatatag gatatgaaga aagaataaac atgcaaaaag
     2041 tatggcaaaa gtatattgat gcttctatat catctacaat aaatatacct tttgaaacta
     2101 cagttgaaga tgttttcaat atatatatta aggcatggga ggctggtctt aaaggagtaa
     2161 caatatttag agatggatgt aaaaggagtg gaattttaat aaatgaaaaa aaggaagaaa
     2221 ataataaaga agataaaaaa gaaaatagta aaaaagaata ttataaaaat aataaaaaag
     2281 aaaaagataa taaagatgaa aaagccaaag aaataataaa taaaaatttt gaagaagatg
     2341 aaaaatttgt gtgccctgaa tgtggaagtg aaggaattgc acatacaggt ggatgtagta
     2401 tatgtttaaa atgtggctat agtggatgca attaaactct aattaaaaaa ttttaatata
     2461 tttaaaaatt aaatttttaa aaacgaaaga tgagggaatg gtatgcagga taaaagtgtg
     2521 agggagatta aagaaataat agagacttta gaagtagaaa aatatatgga atatatagaa
     2581 ctgttaagag ttgatgaaag aaaatctgta caaggtctag ctataaaact agcaaaaaag
     2641 ctggataata ttagaaaaga agaagaaaga ttggaaacta taaatatatt tgagaacgaa
     2701 ggctatgata aaggttattt gtatattgga ggaattgatg aggcgggaag agggccactt
     2761 gctggacctg ttgtagcttc tgttgtagta tttaaaaaag atacaaaaat agaaggtgta
     2821 aatgactcta agaaactgag tgaggcaaaa agagatgaac tttttgaagt aataaaagaa
     2881 gaagcactag attatggaat aggtatagta aataatgaag aaatagatga atttaatata
     2941 ttaaatgcta catatatggc aatgaaaaag gctataaatt gtttaaaaaa agcaccggat
     3001 tatttattag ttgatgcagc aacaatacca ggtattgaca tatctcaaaa tccaattgtg
     3061 aaaggagatt caaaatctat atcaatagca gcagcaagta tacttgctaa agttactagg
     3121 gatagtataa tgtaccaata tgatagagta tacccagaat atgggtttaa atctcacaaa
     3181 ggatatggaa ctaaagaaca ctatgaagct attgaaaaat atggcataac tcctatccac
     3241 agaaaaagtt ttttgaaaaa tatactgtaa atataatatg atataatgta tataatataa
     3301 attactatag ttaatggggg attaaagatg gaatataatg aattattaaa aagtgctaga
     3361 gaaaatttaa atggaagttg taaagtttgt aaggtttgta atggggttgc ttgtgcaggt
     3421 gaagtgcctg gcatgggagg taaaggaagt ggttcttctt ttatagaaaa tagcaagagt
     3481 ttagaaaaag taaaagtaaa tatgagagta atacatgatg tatcaaatcc tgatacatct
     3541 atagagatgt ttggaaagaa aatgagtgca cctatatttg cagctccagt aacaggaact
     3601 actttaaata tgggtggaaa aattaatgaa agagattaca tagaaccagt agttgcagga
     3661 tgtgctaata gtggtattta tgcaatggtt ggtgatacgg cagtagatgc ttttttaatg
     3721 gaaaatttag atgtagtcaa aaagtataat ggagctggaa ttgtatttat taaaccttgg
     3781 gataatgaaa atattataaa aaagataaga ttagctgaag aagctggtgc atttgcagtt
     3841 ggagttgata tagatgcctg tggtctagta actttatcat tacatggaaa accagtactt
     3901 cctaaaaatg ttaaacaaat aaaggagtta gtaaagtcta ctaaactacc atttatattg
     3961 aaaggtataa tgactgttga agatgcttta atggcagtag aagcaggtgt gtatgcaata
     4021 gttgtatcaa atcatggagg aagagtgctt gactgtacac caggagcatg tgaagtttta
     4081 ccaaaaattg ctgatgctgt taaaggtaaa gttacaatac ttgcagatgg tggagtgaga
     4141 actggtttag acgtactaaa aatgataggt ttaggtgcag atgcagtatt aataggaaga
     4201 ccttttgtga ctgcttcatt tggtggtgca actgatggtg tagaaactta tgtaaataaa
     4261 cttcaatcag agctaagtag ttctatgata ttaactggat gtcaaactat aaaagatatt
     4321 gatggaaaag ttatatacaa ataaaatttt atacaaaaaa gaggaaaaat cattttaatg
     4381 tagaataatt gtacaaaggg ggaatttata tgagtagaaa agcttatgaa gaagccttag
     4441 tagaattaga aaaatttatt gatgaaagaa aagaaataat taaaagtgcg gaggactgta
     4501 ttgataagta catagttgac agaacgctac cttttgatta caaagataaa tgtgtggaat
     4561 ggcaacaaga gttacttgat attgcagagg cacaagtttt agaagcaaac gaattaggtg
     4621 ttttactgga agagaaaaaa gaacttgagg aagagtaagt tttattacta aatctaggga
     4681 aaatcaagaa tatgatttac ccctagattt ttttatttaa ataaagatgt ctatataatt
     4741 aatctatctt tatcaatgtt agattaaaag gtttgaagaa aaatagagta attgtatgaa
     4801 tagttaaaca attaaactag tctataagac caatttaatt gttatttttt gaatttagta
     4861 tatggttttt catttcttct aatacaattg tgtaggattt gcattgtgct gcagccaatt
     4921 ctgacaaagc aaacctttga gccttattta gaaagtcctt atctgtgcta catagttgtt
     4981 ttgaattatc ttgtatcata agcatcttaa ttaattttac aatctcaaaa acatctcctg
     5041 aatgaaattt atcatggtat agtttaagtc gtttttttct atcaaaaacc cataaacaat
     5101 caaccttatc aagggattgg ataaagtttt caacttcatt ttcatccata attggacgta
     5161 caccaatctt atcagcattt aaaactggaa tcgaaatttt aagattacta ttgtaaataa
     5221 cagtcaattc atagtatttc tgtatatgat gatttatatc ctttgttaca agaccactta
     5281 tataacaggc tccttctttt ggatacatta cactttcacc gattttatac atatggttat
     5341 acctccaaac tgaattttag atattgcaat ttgggtacaa tgctttaatt atctaataat
     5401 taatattata tcacgaaatg cttgacaagt caaaaagatt aatatataat aaaaagttta
     5461 gaataataga tatatataat tggctataaa aatataagat aagaggattt aatatattaa
     5521 aatatctagt tagtattgta taaaataaca tactaactag atttaataat tattaatttc
     5581 tagcaaaatc tcttaagtta agacttccgg ctgttacaaa taccattcca ataagaatta
     5641 aaacaattat atttattaat atattaccat tattattaat agcatctata gtccaataaa
     5701 atggtgtaag ctttgaaaga ttagccataa aggataattt atcaagtgat ggtatcatat
     5761 tagatatgga tatagctaaa cctattaaag aataaaacgt agacataaga cttatagaac
     5821 cttcgttttt aaacacccta gctatccaaa atacaaatcc agtagcaaca aaactatttg
     5881 ctagtacaat catcaatgta tttagagtca atttaaaatt acttatagat gtgaaacata
     5941 ctaaagttaa ggtgtataca attgcttgag taaaaaataa ccctaaaaat atgctgaaaa
     6001 ttatttttat atcattatgc tttgtagaaa caagtctttt taaggtatta gatttcttta
     6061 atgaaaataa atcagaagct agatgtgctg cattaatgta catgaaataa catatcaaga
     6121 aaactattaa aaatgaattt ttattcttac ctgaactgtt atcaattata tttgtcttgg
     6181 tcaatttatc atctatattt ctatctattt ttaattttaa tgaattagta ataaaagatt
     6241 ctatttgtga ttctgcccaa agactacctc caccattttc agttttaaaa catttaacct
     6301 caggtcgttt taagttatct atattgcttg aaaagttttt atctaaaatg aagacagcag
     6361 aaactttatt attttttaat agatccatag ccttgtcttg ttcgtttaat ccaaatgagt
     6421 tttcttttaa tttagaacta tctattaact tatgttcgta ttttccagaa gaattggata
     6481 caatcgctac tggagaaaag tacattgatt gattttctga gttgccagta aaaaagttaa
     6541 gataaaacat taatataaca ggaaacaaaa ccatattaac taaaagagca tgatttttta
     6601 tatatctttt tatgtttgca atagtcaacc taatcattat taaacctccc tccatttata
     6661 tttttctttt acaatagaaa gtgcaatcaa tactgtagat gcaattaggc atgtaaaaat
     6721 taaactttct attgaatcta tagttttaaa cgtctcataa ttactgaaca atgttcctat
     6781 taaataagta ggagataaac ttgtaaactt agtaaacaca tcatttacta tgctgaaaat
     6841 acctccaaaa actgtttgca agaagagtaa tgcataaact ataactgtac catatttctt
     6901 gctaaaaaag gtagttataa aagtagatat tgaagtcata aaataagaag ctaaagcaca
     6961 aagtaaagct aatataacaa agttaccttt gaatgttaca ccgagtgttc taaaaaataa
     7021 aacgtataat gataaaaata taaatgagta aatccagaaa cctataaaat cataaattaa
     7081 aaattgtact ctagttacag gtatagagta gagtcttttt gaaagtcctt ttgattcact
     7141 tacataattt gataaaatgc tattcattat aaaaattatt gcgaagaatc caagggaaac
     7201 taaagcaaag tactcataag agttttgttt aacattattt tttattatag aggtatcaat
     7261 agaattctta ttaaataatt ttgagaaatc ttctgaactt aatttttgag aattattcag
     7321 atataatttt tcatgataag tatctaatat atcttgaagt aatattgcaa tattatcatt
     7381 agatttaagt ttttctatac ttagattatt tgatttttca tttaataaat tattctcata
     7441 tcctttaggt atagtaagtt ttaagtctgc cttagaatca tctttttgaa ctgttaatat
     7501 agaagataaa tcgtttttta gatatcctgt caaattactt gatagctttg tattatcttt
     7561 atcatatata acaattgatg tcaaatctgt ttttatagga ttttgaaata tactttctgt
     7621 caccatccca agaaatccag ctaaaactag aggtaaggct aagaaatata ttaaattaag
     7681 cataaaggag ctttttaaaa cttttagcat agtttttaca ctaatgtaca atttcatgaa
     7741 atcacctcca ttagtccctt aaatttttac ctgttagatt taaaaatacg tcttctaaac
     7801 taggttcttc ataagatatt cgtgttattt ttgaattatt tttgtcaaca acagataata
     7861 catctgtaag ttgtaatttt ttgtctacac ctaattctaa taatgaatta ttttctaaaa
     7921 tagatacaac acctttagta ttttttaaat caaatataag ttgagcattt atatttttta
     7981 tttccataat taattttgta tttgtagata ctaatgattt taaataatca ttatctccaa
     8041 aagcaatctc tttaccttca tccataataa aaatcttaga acaaagatgc tcaacttctt
     8101 ccatataatg agaagtatat attactgtgg ttttttgctt tttagatata ttttttataa
     8161 aattgaaaat atggtttctt gattgcgggt caacacctac agttggttca tccaatatta
     8221 atattttagg atgatttaaa agtgctattc caatgttcaa tctcctttgc ataccaccag
     8281 ataatttttt gatttttttc tttttaacat cagtgagtcc tgtgacttct aaggcttcag
     8341 ttattctctc ttgaagcaat tttcccttta gtccatagta agtgccaaag tactctaagt
     8401 tatcaaaagg agtaagagtc tctataagtg ctaattcttg tggaactaat cctattatag
     8461 actttgcttt tatacaatct tttgacaaat ctaaaccatt gatttggatg tcgccactat
     8521 ttggtaacat aagatttgta attatattta taagtgtaga tttaccagca ccattcggtc
     8581 caataagtcc aaatatctct ccttctttaa cagaaaagct gatattatca agtgctaatt
     8641 tatcattaaa tctttttgtt atattattta ctcttactat atccataata attcctccca
     8701 aaataatttt gtataagtta aaaatctata atactataat aaagaattat gaatcataat
     8761 tctattacta taagtcacta aaaaaagtga cttaagtcac tttatatttt catttattat
     8821 aaatgagttt gtgagtaaga aatttctagg gtatagattt cttacctata tatttatatt
     8881 acaattttat ttttgaaggc aaatataact agttgtgtcc tatcacgaag ctctagtttc
     8941 cctaagatat tggtgatatt attctttatt gtaccttcag ataagaataa ttcttggctg
     9001 atttctttgt tgttaagtcc atttgcgatt aatctaatta tggatatttc tttttctgtt
     9061 aaattataca tatcagaaat atgtttttgt tctatagttt tttgagggta cataattttt
     9121 tcagccacac ttttatcaag tacaatattt ccaaaaaacg aggaacgaat cccttcatgt
     9181 aatacttcat ccgaactatc ctttaaaaga tatcctgaag ccccaaattt catagcatct
     9241 tctatgtact catcatcatt aaaggttgtc aaaattaata atgctataga gctatcatat
     9301 tcttttataa gtcttgtgcc tagaactcca tcacaaactt tcatccgaat atccataaga
     9361 actatgtcta catcatttgt tttacaaaat tctaatgctt catatccatt ggaagcatct
     9421 cctacaattt ctaaatcgtc ataagatgat aaaataatgt taagaccttt tctaatcaat
     9481 ttttcatcat ctactataag aactttgatt ttattcaaaa aaatcacctc ctgaaaattt
     9541 aggaaaagac gctctaattg tgaatccagt attagaatta tcgaattcaa ctttaccatt
     9601 taattcatat attctttcgc taatactgct aagtccattt cctcttttta tattgttaca
     9661 ccctatacca ttatcgctga tagtaattat aagatttgaa ggattaaatg ttataaaaat
     9721 tctgatttca gttgcttttc catgtcttga tgcgttggaa agagattcct gaacaagtct
     9781 atataaagcg atagattgat ttgtagatag gttccaggta ttttttgata tagttaatct
     9841 aacgttgata tttgtaagtt taataaagct attaaccagt tctctgattt taagtaaatt
     9901 ttggtaattt tcatattcaa tgggttttag ttcagatata gctcttctca catcttgaaa
     9961 actttcagtt acaaactctc taagttcatg aactaaatca gaaatgagag gtttgtcttt
    10021 taagagtttt tctatagcat ttaattggat tatcgtcgta gaaagagaat gtcccacact
    10081 atcgtgtatt tccctcgata tacggtttct ttctttcaaa attgctaact cttctataga
    10141 atttgcataa atctctaaat cggcattagc tttttttaat tcatctttag aaaccctgag
    10201 tttgtcataa agactttgat tgtataattt agataaattc tcgttttcta gatacacaga
    10261 aaagagggtt ataattagta atgtagaact tgattctagt gctaaatata aatttttatc
    10321 taaaccaata ataaaagaaa atattataac taaagagaat aatatatatt tatatttttc
    10381 ctttattaaa aatgaggcat cc
//
LOCUS       DAGQWR010000145        10210 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.146, whole genome shotgun sequence.
ACCESSION   DAGQWR010000145 DAGQWR010000000
VERSION     DAGQWR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.146"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            14..1897
                     /locus_tag="MIW95_003200"
     CDS             14..1897
                     /locus_tag="MIW95_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBY3152505.1"
                     /translation="MFNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2121..2801
                     /gene="pgmB"
                     /locus_tag="MIW95_003201"
     CDS             2121..2801
                     /gene="pgmB"
                     /locus_tag="MIW95_003201"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBY3152506.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2798..5083
                     /locus_tag="MIW95_003202"
     CDS             2798..5083
                     /locus_tag="MIW95_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBY3152507.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNKVASIEKYVSITSTRDYKESELLR
                     KCEDILNIELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNCIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKYIKIDK"
     gene            5309..6202
                     /locus_tag="MIW95_003203"
     CDS             5309..6202
                     /locus_tag="MIW95_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBY3152508.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIAEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEV"
     gene            6250..8550
                     /locus_tag="MIW95_003204"
     CDS             6250..8550
                     /locus_tag="MIW95_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152509.1"
                     /translation="MKKIFSIFISCMVVVTTITGCSSGEVIKNTSPINSVKNLDFSFE
                     VKPEIFEVSVESGGSKEKISEPSKERKVSNFKKNKTEASWTYPDENIDINIKKEDSYL
                     DVNIKSNLEKETSFNWPKVEGESYILPLYEGKFIPSNDKYWKEYLNESTYNVIESFSM
                     QFFAVNKAKYSVIYIIENEFNNVIEFSSKDKINFTFKHEYPSINKNKEYGFKIYLTNK
                     NITDVAKTYKNYIVDKGEFKTLKEKSKENKNIEKLYGAPHIYFGDKSIITINDIEWNK
                     LKENLSDDLTNWMKELLSMKSGNSEFIKVINQIKTQDYIDKYQKNQIVQAFNEVMQMN
                     TFYNEKVFESKDREIKKVLDKGISKLSRVELIEFNKKLLKSKLEESVNDVATWAKYNT
                     TDVINDINKSGIKNAWIGFDDMEEGYISLEMVKRANELGYLVGPYDSYHSIHKPGEEK
                     WSTASFKDKSLYENATIENKKGEKETGFQGEGRKLNPTLSLPSVKERVQSILDEGYEF
                     NSWFIDCDATGEIYDDYSQNHITTQTQDLNARLERMSYIRDHANMVIGSEGGNDFSSS
                     TIAFAHGIETPAFAWVDKDMSKNKGSEYYVGKYYSPTGGVPEIFSKQIPVKERYKKIF
                     IDPIYSLPLFRLVYNDSVISSHQWLWGTLKIKDEVGSRMMKEVLYNTPPMYYIDRTEW
                     EKHKDEIISNNKVWSAFNKKAIKKEMTDFKFISEDRLVQMTEYGEDLKVLVNFSQKDI
                     KYNDEVIKANSLVIYDADSKVIYKPE"
     gene            8784..9275
                     /locus_tag="MIW95_003205"
     CDS             8784..9275
                     /locus_tag="MIW95_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBY3152510.1"
                     /translation="MYENNKVIYNFERCEKLENALYLSQFNTYKNFKLTSKKTVINSL
                     DTSTYNMNIHSISLVDTMECISSEDEQMTGKKLILVGDIDTDVTLDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            9303..>10210
                     /locus_tag="MIW95_003206"
     CDS             9303..>10210
                     /locus_tag="MIW95_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152511.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNSIVFGCIEVEIDYSCKNR
                     SNFKTVNKFNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNP"
BASE COUNT         4306 a          919 c         1792 g         3193 t
ORIGIN      
        1 gggaaatttt aaaatgttta ataaaaataa agaaaatata actttaagta atcaaatcaa
       61 aggaacaatt caaaatagtt taacaaaaga agctatatta catataccaa tgagcaatta
      121 tgcctatggt tatgatagag aaacactaca tataagactt agaactaaaa aaaatgaagc
      181 gaaaagagta attttacgaa taggagacca atatgtgtgg gataaaggag gtgcaggagg
      241 aggaaattta aatgcatctg gagttggttg gtctggtgga acaaatatag taatgagtaa
      301 agaagtagaa actgaattat ttgactattg gatagctaag tgtaagcctt taaataaacg
      361 ctcacgatat ggttttataa tagaaggtca agaagaaaaa cttttattta cagaaaaaag
      421 aataatagag ttgggaaatg aaaatgatga aaaggaatta tgtgaaatag gtaatttttt
      481 tggatatcca tatttaaatt atatagatat acctaaagtg ccaaattggg tgaaagatac
      541 tgtttggtat cagatatttc cagatagatt tgcaaatggt gacccgtcta taaacccaga
      601 aggagtagat aaatggggag ctattccaac cagagataat tttacaggtg gagatttaca
      661 aggtgtgata gaacacttag attatttgag tgatttggga ataaacggaa tttacttttg
      721 ccctattaca attggaaaaa ccaatcatag atatgatacg gtagattata tggaggtaga
      781 ccctacatta ggagataaag agactttaaa aaaactaata gaagaagcgc ataagagaaa
      841 tataaaaata atgttggatg ctgtttttaa tcatatagga tattattcta aacagtggca
      901 agatgtagtt caaaataagg aaaattctcg atataaaaac tggttttata taaaagatat
      961 gagtaaaatt gatactccaa tagaacagat agatgaaaaa aatatacctt atgaaacctt
     1021 tgggtgtgaa aaatatatgc ctaaacttaa tacagaaaat tcagaagtaa taaaatattt
     1081 attggatgta ggcaagtact ggatacagga atttgatata gatgcgtgga gattagatgt
     1141 ttcaaatgaa gtagaccatg ttttttggag aaagtttaga gaagaagtca aaaaagtaaa
     1201 accagacatt tatatattgg gagaaatttg gcatggtagt ttaccatggc taatgggtga
     1261 ccaatttgat tcagttatga attacttgat gtcagaagcc atgaaaaaat tcttttgtac
     1321 agatgagata aacgccgaag aatttaagta tatgataaat gatgtaatgg taagttatcc
     1381 aatacaagtt agtgaagtta tttttaattt acttggaagt cacgatacta caagaatatt
     1441 gacttatgct aatggaaata ttgatagatt taaacttgca tatatgttta tgtttgtaca
     1501 aacaggatgt ccttgtatat actatggtga tgaaatcgga atggaaggag aattaagttc
     1561 aatatctgaa ggtcaaagaa aatgtatgga atggaatgaa gaaaaatgga ataaggatat
     1621 atttgatttt atgaagaaga ttattagact tcgtaaagaa aataaagaac ttaggtctat
     1681 atcaaatgaa tgggtgttag cagataagga aaatggaacg attatattga aaaaagaatc
     1741 tatttctata ataataaata attcatctaa aaatataatt ttgacgttac cagattattt
     1801 agcaaataaa aaagtaaaag atttatatga agaagaaata gttgatttaa aagaagaact
     1861 aatgttagag aaatacaagt tcattatttt aaaataatac aaaaagtatt tattttagat
     1921 ttcatgttaa attattatac agtttaaaca agtctttata aaacataaaa aaatatggtt
     1981 tacaagtgag tgaattgaaa gtagatttta aattaaagac tgagttagag tagataattt
     2041 tataaataat atcttaaata caagatagaa aataaattga ttattagtct aaaaatttct
     2101 tgtagaaaga ggaaataaga atgattgaag catttatatt tgatttagat ggtgtaatta
     2161 cagatactgc atattatcac tatatggctt ggagaaaact agcccataaa gttgggatag
     2221 atatagacac aaaatttaat gaatctttaa aaggcataag tagaatggag tcattggaca
     2281 ggatattaga atttggaaat aagaaatatt cattttcaga agaagaaaaa gtgagaatgg
     2341 ctgaagaaaa aaataattac tatgtatcat tgatagatga gataacttca aatgatattt
     2401 tgccaggcat agaaagcttg ttaattgatg taaaatctaa taatataaag ataggtctat
     2461 cttctgcaag taagaatgca ataaatgtat taaatcattt gggtataagt gacaaatttg
     2521 attttatagc tgatgcagga aaatgtaaaa acaataaacc acatccagag atatttctta
     2581 tgtcagcgaa agggcttaat gtaaatccac aaaattgtat agggatagag gatgcaagtg
     2641 caggtataga cgctataaac tcagctaata tgttttctgt tggtgttgga aattatgaaa
     2701 acttaaaaaa agcaaattta gttgtagatt caacaaatca attaaagttt gaatatatac
     2761 aagaaaagta taatgagtat atagttagga gaattatatg aaatgtttat ttagtaatgt
     2821 agatgagtgg aagattatac aagataagat tgaaatcaaa gaaaatagat tagcagagtc
     2881 cataatgagt ataggcaatg gatatatggg tatgagagga aactatgagg aaaattatag
     2941 tggagataca cacaaggggt cttatatagc tggagtatgg tttccagata aaactcgtgt
     3001 tggttggtgg aaaaatgggt atccagaata ctttggtaaa attcctaatt cagtcaacta
     3061 tataggtgta agaattttca tagatgatgt agaattagat ttagcaaaat gtaaagtaga
     3121 agatttttat agagaattag atatgaaaaa tggtatttta aatcgtaggt ttattgtaaa
     3181 tataaatgat aaaaaatttg aagttagggt tactaggttt ttaagtatat ctgttaagga
     3241 acttgcagta attaaatata gtataaaatc attaaattct aatactaaaa taaaatttgt
     3301 accatattta gattcaaata taaaaaatga agattcaaac tatgaggaaa agttttggaa
     3361 tggaatttct tcaagttctt tagaatataa aggtagtatt ctttctcgaa caaaggagaa
     3421 tgcatttaat acaccagtat tcactgtggg ttcaatgatg tatattaaga caaatggtga
     3481 tgtagaagct gtatctcatg agagtgatta taattactgt gaaaattcga taacaatgga
     3541 tgttttaaaa aataaggttg caagtataga aaaatatgta tctataactt caactagaga
     3601 ttataaggaa agtgagctat taagaaaatg tgaagacatt ttaaatatag aactatctaa
     3661 aagttatgat gatatattaa atgaacatac aaagttatgg aataaaagat gggaaagtgc
     3721 agatgtaaaa ataaatggag ataaaagtag ccaacaaggt ataagatata atttattcca
     3781 actattttca acatattatg gagaagattc gcgattaaat ataggtccaa aaggatttac
     3841 tggtgaaaag tatggaggag ctacttattg ggatacagaa gcattttgtg ttccagtata
     3901 tctaggtgta gcagataagg aggtaactag aaatctttta gaatataggt acaatcagtt
     3961 agaacaggca aaggataatg caaaaaaact agggcttaaa ggtgctttat atccaatggt
     4021 aacatttgat ggagtagaat gtcataatga atgggagata acatttgagg aaatccaccg
     4081 aaatggaagt atagtatatg caatatataa ctatacaaat tatacaggtg actataaata
     4141 tataaaagaa aaaggaatag atgtaataat agaaatagct agattttggg caagtagagt
     4201 tcatttgtct acaaaaaaag atttgtatat gatacatgga gtaacaggtc caaacgagta
     4261 tgaaaacaat atcaataata attggtatac taattgtata gctaaatggt gtttagatta
     4321 tgctgtagaa aatatcttac gattagagaa agagtgtaat gaatgtattg acagaaataa
     4381 tgtgactagg gaagaagttg agttttggaa aataatatca aataaaatgt atctgccgta
     4441 tgatgaggaa ctacaaataa taattcaaaa tgataatttt ttagataaag aatttgtaga
     4501 agtaaaggat ttaccaaaag aaaattttcc tcttaatcaa aattggtctt gggataagat
     4561 tcttaggtcg tgttttataa aacaagcaga tgtattacag ggaatttact attttggaaa
     4621 tagatttact aaagaagaaa aaaagagaaa ttttgacttc tatgaaaaat atactgtcca
     4681 tgaatcttct ttatcttcta gtatttattc tattattgcc tcagaaatag ataacctaga
     4741 aaaggcttat gaattatact caagaacagc taggcttgat ttagataatt acaataatga
     4801 tacaaatgat ggactgcaca taacttcaat gtctggttca tggctttcca ttgtacatgg
     4861 atttgcaggc atgagaacat ggaatgaaac tctatctttt gaccctaagt tgccaaaaga
     4921 gtggaaaaat tattctttca atataaatta tagagataat attattaatg tagatgtatg
     4981 tagtgattta attaaaatag aaaatttaaa aggtaaacca ataaaaatta ttgtatatgg
     5041 gaaaaaatat atattagaaa aatatataaa aattgataag tagatttaat aatattttta
     5101 ttatataaat agttaaaata agtttattta attatttatc tattttaact attttgtgtg
     5161 atagaaaact atatattata acaatagtag tgcctttaat taaatatatt tcataaatct
     5221 tcataaaatc ttcaaaattt cgtaaagaat atgtcacaaa agtaaggtat attatattca
     5281 gcagatagta taagggagga caatatttat gaagaataaa aaattcattg ttataatact
     5341 gataatatca attttaatag gtgtattagt gaaggaatat tcatctagag agataaaaaa
     5401 ggatgatata aatgtcagta agtatataaa atatgctgat ttagcaagta aaaataatgc
     5461 acaagttaac tggaagtatg tagcttccat tgttgctgtg ttaaataaaa ataacttaaa
     5521 aaacgttaag gattcacaga tacaagaagt atcagattta tttgttaaga atttttctaa
     5581 aaataataaa ataaataagt taagtgatat tttggatgag ttagaattta gtaatagaca
     5641 aaaaagattg gttgataatt atatagacaa tcttaaggat tatggtataa aaccagaaag
     5701 attgaagtct gatacaaaat atatgaagtt tattgctgaa attaaaacag aagcaataca
     5761 aaattataag gattataaaa tacttccatc tataactatt gcacaagcta taatagaatc
     5821 tagctggggc aagtcaactt tggcaaaaca atataacaat ctatttggta taaaggctga
     5881 cgcttattgg aaaggtaagt cagtgacttt agaaacgaaa gaacatttag atacaattat
     5941 agatgataaa tttagaatat atgatgataa aaatgaatct ataaaagacc atgcaaaatt
     6001 tttagccaca aataaaaggt ataaaaacaa tggtgtattt gatgcaaaaa cgtatatata
     6061 tcaagctaaa gcattagaaa aggcaggtta tagtactgcg aaggatgaaa atggcaatag
     6121 tatatatgca gctcgtctaa tagaattaat tcaacagtat aatcttcaat taatagacag
     6181 cgaaattcaa agtgaagtgt aacttttatg taacatagga gtgatatatt tatcgaggag
     6241 gtggttttta tgaaaaaaat attctcgata tttataagtt gtatggttgt tgtaactact
     6301 attactggat gtagtagtgg tgaggtgata aaaaatacat cacctatcaa tagtgttaaa
     6361 aatttggatt ttagttttga ggtgaaacct gaaatatttg aggtatcagt tgaatctggt
     6421 ggttcaaaag aaaagatatc tgaaccctca aaagaaagga aagtatcaaa ttttaaaaag
     6481 aataagacag aggcatcgtg gacgtatcct gatgaaaata tagacataaa tataaaaaaa
     6541 gaggacagct atttagatgt aaatattaaa tctaatttag aaaaagaaac tagttttaat
     6601 tggcccaaag ttgaagggga aagttatata ctaccacttt atgagggtaa gtttatacca
     6661 agtaatgata agtattggaa ggagtatcta aatgagtcaa cttataatgt tatagaatct
     6721 ttttcaatgc aattctttgc agtaaacaag gcaaagtatt ctgtgattta tattatcgaa
     6781 aatgaattta acaatgtaat tgaatttagt tctaaagaca agattaattt tacatttaaa
     6841 catgaatatc caagtatcaa taaaaataag gagtatgggt ttaaaatata tttgactaat
     6901 aagaatatta cagatgtagc aaaaacttat aaaaattata tagttgataa aggagaattt
     6961 aaaacattaa aagaaaaatc taaggaaaat aaaaatattg aaaaacttta tggagcacca
     7021 catatttatt ttggagataa gtctatcatc acaataaatg atattgaatg gaataaatta
     7081 aaagaaaatt tatcagatga tttgactaat tggatgaaag agcttttaag tatgaagtct
     7141 ggcaatagtg aatttataaa agtaattaat caaataaaaa ctcaagatta tatagataaa
     7201 tatcaaaaaa atcaaatagt acaagcattt aatgaagtta tgcagatgaa tactttttat
     7261 aatgaaaaag tttttgaaag caaagataga gaaataaaaa aagtattaga taaaggtata
     7321 tctaaattaa gtagggtaga acttatagag tttaataaga aattattgaa atcaaaattg
     7381 gaagagagcg tgaatgatgt tgctacatgg gcaaaatata atacaactga tgtaattaat
     7441 gatattaata agtctggtat taaaaatgcg tggataggat ttgatgatat ggaggaaggt
     7501 tatatatcct tagagatggt taaaagagct aatgagttag gttatctcgt agggccatat
     7561 gattcatacc actctataca caaaccaggg gaagaaaagt ggagtacagc atcatttaaa
     7621 gataaatctc tatatgaaaa tgctaccata gaaaataaaa aaggtgaaaa agaaactgga
     7681 tttcaaggag aaggaagaaa actaaatcct acattgtctt taccaagtgt aaaagagaga
     7741 gtacaaagta tattggatga aggatatgaa tttaattctt ggtttataga ttgtgatgct
     7801 acaggagaaa tctatgatga ttattctcaa aatcatataa caacacaaac acaagattta
     7861 aatgctagac ttgaaagaat gtcatatata agagaccatg caaatatggt cataggttca
     7921 gaaggtggaa atgatttttc aagtagtact atagcatttg cacatggtat tgagacacca
     7981 gcatttgctt gggtagataa agatatgagt aagaataaag gcagtgaata ttatgttggt
     8041 aaatactatt ctccaacagg aggagtacca gaaatatttt caaagcagat acctgtaaaa
     8101 gaaaggtaca aaaaaatatt tatagaccca atttactctc tacctttatt tagattagtt
     8161 tataatgatt ctgtgatatc ttcccatcag tggttatggg gaactttgaa gattaaggat
     8221 gaagttggaa gtagaatgat gaaagaagta ttatataata caccacctat gtattatata
     8281 gatagaactg aatgggaaaa acataaagat gaaataatct caaacaataa agtatggtca
     8341 gcgtttaata agaaagctat taaaaaagaa atgacagact ttaaatttat ttctgaggat
     8401 agactagttc aaatgactga atatggagaa gatttaaagg tattagttaa tttttctcaa
     8461 aaagacatta agtacaatga tgaagtcata aaagcaaatt ctttggtaat atatgatgct
     8521 gatagtaaag tcatatataa accggaataa tagatttaat gaaaataaag attgtttaaa
     8581 tttcaagtaa aaaataatat aatattcatt tagtttgtat taaagtagtg actatattaa
     8641 tctggataat tagtatagtc actattattt atattctttc ctaaaagtaa gtagtaactg
     8701 aatcgtgaat aatcaaaaat ggtaatttga aatagttttt atgaatagta ttaatatata
     8761 gatataggga gattgatgtg attatgtatg aaaataataa ggtaatatat aattttgaaa
     8821 gatgtgaaaa gctagaaaat gctctttatt tatctcaatt taatacatat aaaaacttta
     8881 agttaacttc taaaaaaact gtaataaact cattagatac atctacttat aatatgaata
     8941 ttcatagcat aagtctcgtt gatactatgg aatgtatatc gtcagaagat gaacaaatga
     9001 caggaaagaa gttgatttta gttggcgata tagatactga tgtaacatta gattatgcag
     9061 gcaataaaag aaatagaaat tctagtaaaa aaaatacatt taaattaaaa atacccttta
     9121 gtacatttat acaaatgcct agaaagatag aaaataaaga taaaattaat ttaaagtatc
     9181 ttatacaaga tattacttca tctattttag atgataattt atttattagt gttactgcaa
     9241 ttataagtta tgaaaatagt tccaagatag agtaatcttt aaattataga aaggtggatg
     9301 caatgagaat aacgaaaact acttttaatg atgtattttc aaatgatgca aatggaagta
     9361 ttataaaagt agaaaaatat gttgataaaa aatgtgcatc tgtatttgag attttgacct
     9421 acactattat tgttacaaat acaagtaggt acaaaactgg aaatatattt ttcaaagatt
     9481 atatttctaa atatatagag tttataaata atactgtaaa agttaatggc attataaaac
     9541 gtggattaga cccacagcaa ggtttttaca tagggagaat agatgcaagt tgtaaaaaaa
     9601 taatatcttt taagagtgta gttttaccaa attctgcaca taggattata aagaatagtg
     9661 ctaatatata ctattattat aagtgtaatc tagataaatt cccaacaaga atatcaatag
     9721 aaagtaatag agtttatact aatgtaaata aaattgtatt caaacaattg aatataagta
     9781 atactttaaa agctcctaaa aatctaagag atatattaaa agtaaatgta aatagtaaaa
     9841 tatttgatgt taaacctatg aaaaatctta taaacaaagg tagtaatttg aaattatgta
     9901 actcgatagt ttttgggtgt atagaagttg aaatagacta tagttgtaaa aataggagta
     9961 attttaaaac agtaaataag tttaatgata ttaaaaaaga taagtattat aaaaatgata
    10021 tacaaaataa caatgaaaat ataaacgatt gtaaaaagca aataagtttt ggtgataata
    10081 aggtaaaaaa aataacaaaa acttttggat tttcttgttt catttgttct ccagttggaa
    10141 ttatttatga agatatgaaa aatataaata taaaaattga acatacatca ataaatgaat
    10201 taaatccaga
//
LOCUS       DAGQWR010000146        10151 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.147, whole genome shotgun sequence.
ACCESSION   DAGQWR010000146 DAGQWR010000000
VERSION     DAGQWR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.147"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..707
                     /locus_tag="MIW95_003207"
     CDS             <1..707
                     /locus_tag="MIW95_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBY3152512.1"
                     /translation="EDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKR
                     DILSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLN
                     MNPKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKE
                     GKSYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDAD
                     LEDNRINYINSIGMII"
     gene            708..1151
                     /locus_tag="MIW95_003208"
     CDS             708..1151
                     /locus_tag="MIW95_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152513.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYINQNKLVDKSKHTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            1166..1750
                     /locus_tag="MIW95_003209"
     CDS             1166..1750
                     /locus_tag="MIW95_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152514.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            2003..3040
                     /locus_tag="MIW95_003210"
     CDS             2003..3040
                     /locus_tag="MIW95_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBY3152515.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            3121..3486
                     /locus_tag="MIW95_003211"
     CDS             3121..3486
                     /locus_tag="MIW95_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152516.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINVAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            3491..3808
                     /locus_tag="MIW95_003212"
     CDS             3491..3808
                     /locus_tag="MIW95_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152517.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            3856..5460
                     /locus_tag="MIW95_003213"
     CDS             3856..5460
                     /locus_tag="MIW95_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152518.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVVLKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYINVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            5584..7212
                     /locus_tag="MIW95_003214"
     CDS             5584..7212
                     /locus_tag="MIW95_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBY3152519.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIAKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            7477..8826
                     /locus_tag="MIW95_003215"
     CDS             7477..8826
                     /locus_tag="MIW95_003215"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBY3152520.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            8982..10061
                     /locus_tag="MIW95_003216"
     CDS             8982..10061
                     /locus_tag="MIW95_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBY3152521.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
BASE COUNT         4231 a         1011 c         1804 g         3105 t
ORIGIN      
        1 tagaagatgt agttagattt gtaaaagagg agattggtaa atcaaaaata ttatataaaa
       61 atttatactt taacattcaa attcaagata tagtcattag aaatgttgaa gttctaaata
      121 ctaagaaaaa aagagacata ttaagcatga ttgaatttga aataacacaa tacattccaa
      181 taaacataaa taactattca ataaaatata aagttataaa ttctaaaaat gataaattga
      241 atatacaagt aattcttttc ccaaagagta ttattcaatt aattaagaac atttcagaaa
      301 atctaaatat gaatccaaag actataaata taaattttga tatattacaa aagcttataa
      361 acttaaattt aatagagaat ttttcagaag aaggggtatt tatagaatgt aaaaaaagtc
      421 agttaattat aaatataaca aaagataaaa aaataagaga aacatacttg ctatcaaaag
      481 agggaaaatc atatgagttc ataaaaagat tattaaggga ttttaagtat gtttattact
      541 atggtataga aaattgtttt attgaagaat actttaaata tacagataaa tctattgaag
      601 taaatccatt aagattaaaa gataaagtaa aagtagaaaa taattttgat gcagatttag
      661 aagacaatag gataaattat ataaatagta taggaatgat tatttaagtg aaagtggata
      721 aaacaataaa ttttcttaat ggatttaaaa aaactaaacc atgtaataaa atctataaaa
      781 taataatagt cttaatttta acgtgtattt tatttaaatc atttatgtat gtgaaatcta
      841 taaatgaact tgaagattat ataaatcaaa ataagctagt ggataaatca aagcacactt
      901 taccaacaac agataaaact agagagaaat tttctaaaat aaaacttaat gaaataaaaa
      961 aaatatatga aacaattgga ataaatagta ttactaaaat atcgatagat aaaaataagc
     1021 tagagatgga aggtgtgtgt agtgatttag atgttttaaa caacctaagg aaaataaagt
     1081 acataaaata ttattacata gacagtattg taagagatgg agaatattat agatttagaa
     1141 taaaaagtta gataggtgat tatttttgag attaaataat agagaaaaaa atctacttaa
     1201 aatattgtta atattagtta ttatattggc aagtgatata gcagtaagtt atttggaaga
     1261 aaacctaact agtttgaaga atgaaaagca aaatataata aataaaataa ataatcaaga
     1321 taattatcaa ataaaggata ataagcatat agatgataaa tatatagaaa gtgttgaatt
     1381 aaataaaaat gaagaattga aaaataattt taataactca tttttagata aaaaaacaaa
     1441 taaagatata gtgttattag tagaaaatga actgagagat attgtgaatg tagaatatat
     1501 taatcaagaa agtagtttag ataatcacaa taaaaaacaa gttaatttag aaataaaagt
     1561 taatggagaa cttgcaaatg tgttgaaact tgaaggtgta atgaagaagt taagactaaa
     1621 taataaactt gaaaagatag aaattaacaa attaaaagtc aataattttg atgttcaaaa
     1681 tattccaaat gaatataaac aagaaaaaga caatattgtt gaatgtaaaa tgacagttag
     1741 agtcatataa caaaaaagaa tagataaagt aaaaaactat tatcaattaa taaaatttta
     1801 tagaggaatt agttttacga taattcattt ctacaagagg gattgttgat gattttatcg
     1861 aaatcctaat taaagttaat atattgtacc aaaatataag tgaatttaat agaatattta
     1921 cttatactat agattgatta ataaaaaaat ggtataataa caaattgtac tttaatttaa
     1981 taataaggga gtggaagtca tattgttagg gagaaatgag ctatgccctt gtggaagtgg
     2041 caaaaaatac aagaaatgtt gtttaaataa agatattgtt tcagaaagag catgcagaaa
     2101 aactgaactt tcacaaaaac aatatacaga tttatattca aagttatatg attattcaag
     2161 acaggataag tttaaagaag aatgtgaaaa agctaaagaa atgttttata taatgcaaaa
     2221 tgatagtgta aatgaaaaat ttgaaagatt ttttaatact tatttaatac aagaccacat
     2281 aatggaaaat aaaaaggtta tgacagttgg ttttcttgaa gaaaatggtg caaacctaag
     2341 tcaatcagaa gtatcaatat taaaaagtat gtttgaatca tacgtaagta tttatgaggt
     2401 aaaagaagtt tctgctggaa aaatagtatt aaaggactgt ttaagtggaa atgaactttg
     2461 tacagaagat gtaaaacttc tgagaagttt taaaatcgga agttgtatga tagctagaat
     2521 agttgatatt gaaggaacta acatattgat agatataact ataagtatat caaatgaggt
     2581 taaagatata attttaaagg atattatgaa tctattcaat caatatcaag atgtatacaa
     2641 agatatgaag tcgttcttaa tttatcatac acatatacta tacaagtata tacaacagct
     2701 tttagaccca agtatagctg aatatttaaa gagagaaaga gataaaaacg atacaaaagt
     2761 taattcagaa aatgaattag ctgaagaaga atgtaaagtt ttagatactc ttaaagaaaa
     2821 tgtagaaaaa gaaaatatag atgcatgtat agagttttgg aatgaatata agaaaaatca
     2881 tgaaaatata aaaggagctg aaaatggttg ggcagctgct gttgaatact atgttaaaaa
     2941 agaagcagga caacctataa ctcaagttca aatatcaaag aaatacgata taagctctag
     3001 tacactagga aagagataca aagatttaaa aatatcttaa ttaattataa aaagctttaa
     3061 gtcatagata aagaatctat agtataatat atgtataatg tttagaaaga ggtattaata
     3121 atgagtctta gtttaggaaa tgcaattgct gtatcaacga actttataaa atctaaccca
     3181 gctgtacctg tgtacaacgg agaagaaata tatgcagtta tttatcatat agaaatagga
     3241 tataataatg gtgtatcacc tgataccaaa caatatataa taatagacta taaagagttt
     3301 gatttatcta tggataaaaa aaatgaacta ataaatgttg ctaaagagta ctgtaaatct
     3361 aaaggtattg tagaaaacga aaaccttgaa ataaaccttt tagacaatga agaagcagag
     3421 aacttcttag aaaaggtgtt taataacatg aaggttataa gaggtcttat aactaaaaaa
     3481 ggataaataa atggactata taggaataga aaatataaca ccttatgaaa atacatatga
     3541 atttagtgta tatgaatatg atgatgaaat caccttaggt agtgaaaagt tatatgtgtg
     3601 tgaattaagg gttgtattga ttaaagttaa ttctctgtat gttgaaagat tgcataaatc
     3661 agttgaagca atggtcttag taaaaaactt gaaaaaggat ttagataaaa cacttgttgt
     3721 aaacaaaata aagaattttg tgctagatga gatttgggta gaaaatctag taaaagagaa
     3781 tatagaagtt atatttgtag aaagctagga ataaccctag cttttttata ttacaatcta
     3841 agagaggaaa acaatatgtt aagagtatct aatgtaaaac ttggaattga taaagatata
     3901 tctttattaa aagatgttgt acttaagaag ctaaaaataa aagaaaaaaa cttagtcaaa
     3961 tatagtatat ataaagaatc tatagatgct agaaaaaaag gaaaaatgga atttgtttat
     4021 tctgtagatg tagaagtcaa agatgaaaat aaaatactta actcaaaaat aaaagatgta
     4081 acaaaaataa aggaaatcag atacataaat gtacctgtgg gtgataaaaa attaaagaat
     4141 aatcctttgg taattggtag tggaccagct ggtttatttg caggtctgct tttagctcaa
     4201 atgggttatg aaccaatcat acttgaaaga ggtcttgatg tagacagtag aaccaaagat
     4261 ataagtgatt tttggacaac tagaaaatta aaaaataatt caaacgttca atttggagaa
     4321 ggtggagcag gaactttttc ggatggtaaa cttacaacta gaataaaaga tatacgatgt
     4381 agaaaagtat tagaagagtt agttcagttt ggagcaccag atgaaatatt atactcacat
     4441 aagccacatg ttggaacaga tatattaaaa aatgtagtta aaaatataag aaatgaaata
     4501 ataagtttag gtggtcaagt tagatttaat tcaaaagtaa cagatatagt tgtatcaaat
     4561 gattctatac aatctgtaat aataaatgat actgaaaaaa tagatacaga tacaattata
     4621 ctagctgttg gacatagtgc aagagatact tatgaaatgt tgtattcaag aggtataaaa
     4681 atagttcaaa aaccttttgc aataggagct agagttgaac atccacaaga tttaataaat
     4741 aaagcacagt acaaggattt ttataatcat cctaggttag gagctgcaga ttatagactt
     4801 atagagcatg tttcaaatgg aagaacagca tatacttttt gtatgtgtcc aggaggtagt
     4861 gtcatagcat ctgcatcaaa tgattttgaa atagttacaa atggtatgag tgaacatgca
     4921 agagataaaa taaatgcaaa tagtgcattt ttagtaaatg taacgccaga agattttaag
     4981 agtgataatc cactagcagg tgttcatttc cagcagaagt atgaaaaatt agcttttgaa
     5041 ttaggtggaa aaaattacaa tgcaccagtt cagcttgttg gagattttat aaacaataag
     5101 acttctttga atttaggaag tgttgagcca tcatatagac caggatatac acttacaaat
     5161 ttaagtgaat gtttgcctag cttcgtaaca gagactatga gagaagcgtt tatatctctt
     5221 gataaaaaat tgaatgggtt tgcaatgtat gatgctgttt tgacgggagt agaaactagg
     5281 tcatcatcac caataagaat agtaagagat gaaaatactt tagaatcagt gagtattaag
     5341 aatctatatc catgtggtga aggcgctgga tatgcaggtg gtatagttac agcagcagtt
     5401 gatggtataa aatgtgcaga aaaaataata caaaaataca ccaataatac aaaaatttaa
     5461 cataaactta atgatttttt aatatttaac atgaatttaa cattatcact cctataattt
     5521 gataaaatac aataggttat aaaattttta gatacagtaa tctctttaca aggaggaaat
     5581 aatttgaaca ttataataag tctaatgggt ggattaggtc tgttcttata tggtatgaac
     5641 cttatgggag aaggtcttca aaaatctgct gggacaaagc taaaaaagat aataaaacta
     5701 cttactagta acctatttat gggagttttg gtaggtacag gagttacagc agtaatacaa
     5761 agttcaagtg ctactactgt aatggttgtt ggtttcgtaa atgcaggaat aatgacttta
     5821 aaacaggcaa taggagttat aatgggtgct aatataggga ctactgttac agcccaattg
     5881 gtatctttcg acttaactgg tatggcccca gtagcattag gtgtaggtat tatactttat
     5941 ttatttggaa ataagcctag aataaaaaat atagccgaaa tacttatagg ttttggtata
     6001 ttatttacag gtatggattt tatgaagatg gcagtggagc cactaagaga ctatcaagga
     6061 tttactgacc tacttgttac atttggtaga tatccactac ttggattatt attgggattt
     6121 gggattacag caataataca aagttcaagt gcatctatgg gtatgttggt tgcactggca
     6181 gcagaaggtc tagtgccatt atcagcagca ctgccaattc tttatggcca aaatattggt
     6241 acatgtgtaa cttcactgtt atcaagtatt ggtgctaaca aaaatgcacg aagagcagct
     6301 atgatgcatc taatcttcaa tgttttagga acggtgatat tcttaatatt cttgaataaa
     6361 ccagtagtaa gcatggttac aagttgggat ccaagcaatg ttgcaagaca aatagccaat
     6421 acacatacat tgtttaatat aatatctgta ttgatacttt taccatttac aaatcttatt
     6481 ataaaactag ctataaagct tgttcctgat agagcaggtg atatagatga agatgaaact
     6541 aaaactatta aatatataga tgatagaatg atagaaactc cttctatagc tcttgcaaat
     6601 acgataaaag aagcacttcg tatgggtgag aaggcgaagg aaagcctaaa tgcatctatg
     6661 gaagccttag tagaacattc aactgaaaaa atagctaaga catatagacg agagagatta
     6721 atcaatgatt tacagaaagc tattttaaat tacttactta aactttcaaa agcaccttta
     6781 gatgatgatt caagagaagt tgtggatact ttgtttaata cagtcaatga tatagaaaga
     6841 attggtgacc acgctaaaaa tatagctgaa ttatcgcaag ttgcaataga ttcaaatatt
     6901 tctttctcag aagaaggaca atctgaactt gatgttatgt ataatagagt tgtttcagca
     6961 tatacttatg cattagaatc aatgagaact gataatgttg atttagcttg taaggttata
     7021 aaaattgaag aacaagttga tataatggag aaatcttgta gagctaatca catgtataga
     7081 ttgaacaata atctatgtag tatagaaaat ggagttattt atttagacgt aataagtaat
     7141 ttagagagaa tatctgacca tgctgtcaat atagcacaac aagttatagc gaagagatta
     7201 ggaaatgatt aaaaataatt aatttaaaat aatataaaat ttaaaataga ggataaataa
     7261 aagagaaact ttaaatgata cgtttagagt ttcttttttt gtaaatttat ttgactaaaa
     7321 tttttaaatt tatacatagt tatagtattt agaagaatcg atttcctgat aataaatgat
     7381 gagatatgta tgattttgtg gcaaactttt aaaaaaaaca cctatatttt gtgttataat
     7441 ctctttaaaa cgaatatgct gaaaagaggg atttaaatgg gaaaaataaa ttttgattat
     7501 agtaaagcta cagagttttt ttgtcaaaac gaaatagatg tcatgcaacc atatgtagat
     7561 gttgcgcacg acatgttaca taataaaact ggtttaggaa atactttttt aggatggata
     7621 gaccttccta aaaattatga taaagaagag tttgacagaa taaaaaaatc tgctgagaag
     7681 ataaaatcag actcagatgt tcttctggta ataggtatag gaggctctta tttaggttcg
     7741 agagctgcta tagatatggt aagtcattct tttaggaatg gacttaaaaa agagcaaaga
     7801 aaggctccag aagtatattt tgtagggcat aatataagct caacatatat tatggattta
     7861 ttagatataa ttgaaggaaa ggatatttct gtaaatgtaa tatctaaatc aggaactact
     7921 acagaaccag ctctggcttt tagaatattt aaagattact tagagaaaaa gtatggtaag
     7981 gaagaagcaa gaaagagaat ttatgcaaca acagatgcaa gtaaaggtgc acttagacag
     8041 ttagctacag aagaaggata tgagactttt gtaataccag atgatgtagg tggtcgtttt
     8101 tctgtattga cagcagttgg gttacttcct attgctgctg ctggacttga tattgatgct
     8161 atgatgaaag gcgcaaatga tgctagagaa gcatttcaaa atcctgactt aaaaagtaat
     8221 gatagttata gatatgctgt tgctagaaca attttacata gaaaaggaaa agatgtagag
     8281 ttattagtga actatgaacc tcaactacat tatgtaagtg agtggtggaa acagctttat
     8341 ggagaaagtg aaggaaaaga aaataaaggt ttattccctg cttctgtaga tttctcaaca
     8401 gatttacatt ctatgggtca atacatacaa gatggaaaaa ggcttttatt tgagacagta
     8461 cttaatgttg aaaactgtaa gagaaatata actataagtt cagaggaagt agacttagat
     8521 ggtttaaatt atttagcagg taagactgta gattttgtaa atcataaagc ttttgaagga
     8581 actctattgg ctcatacaga tggaaaagta cctaatttag taataaatat acctcaacta
     8641 gatgagtata attttggata tttagtttat ttctttgaga aggcttgtgg aataagtgga
     8701 tatttattgg gagttaatcc atttgaccaa ccaggagtag aagcttacaa gaaaaatatg
     8761 tttgctttac taggcaaacc aggttatgaa aaagaaaagg aagagctaga aaaaagactt
     8821 aaataatttt aaatagttaa ggtttaatta agattaaaat aatataatga aatcatcgaa
     8881 aaggagttta ctcaaaatag atgatttcat tttttttatg aaaaattggg aaaaatatat
     8941 tttgataatt tatattatat taagataggg agtggtatga gatgtcaaga agaaagaaag
     9001 ggataagttt agtaatatta gttgctataa taagttcaat acttagtagt tttctaacaa
     9061 taattttagt taaggacaat ttagtaagca aatctacagg aagttctacg ccgatagttg
     9121 taaatgatga tggtaagagt caaaatattt atcaagcagt tgcagaaaaa gctacgccat
     9181 ctgttgtagg tattacaaca actagtgttg atactagtaa tatgtttgca ataccaactg
     9241 aaacacaagg tgtaggaact ggaatcatag ttgattcaaa tggatatata cttactaatt
     9301 ctcatgttat atcagatggt caagctacaa gtgtaaatgt attattcaat gatggttcaa
     9361 caacttcagg taaagtagta tggtttgacc aacaacttga tttagctata gttaaggttg
     9421 ataaaactgg actaacacca gcagaatttg ctgatagtga caaagtaaaa gttggagata
     9481 tatcaatagc aataggtaat cctcttggat tagattttca aaaaacagtt actcaaggga
     9541 taataagtgg attagataga actatacaaa cagaaaaaac taatatgact ggtttattac
     9601 aaactgatgc aagtataaat gcaggaaata gtggaggacc attgttaaat caaaaaggtc
     9661 aagtaattgg tataaacaca gcaaaagctt ctcaagcaga aggattagga tttgcaatac
     9721 caatcaatac agctaagagt atagttgaag aagtaatcaa aaatggaaaa tatgaaaaag
     9781 taactttagg cataaaaggt acagatgtat caaattatga agcagcaaca ggtacaaaat
     9841 taagtactga taaaggtgtt tatgttgcag aagtaatatc tggttcatca gcagaaaaag
     9901 ctggagtaaa ggtaggggat attataacta aagtaggaga tacagatata acaggtatga
     9961 atgatttaaa taaaaagttg tatactttct caaaaggtgc tagtacaaaa ataacagtaa
    10021 atagaggcgg aaaagcagta acaataaatg tgaatttcta aatatgacca gaaagaagga
    10081 gactacatgg taaagtgtag tctctatttt tatttaattt ttatagtata tacatctaaa
    10141 ttaattaaaa t
//
LOCUS       DAGQWR010000147        10102 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.148, whole genome shotgun sequence.
ACCESSION   DAGQWR010000147 DAGQWR010000000
VERSION     DAGQWR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10102
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.148"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            122..1036
                     /locus_tag="MIW95_003217"
     CDS             122..1036
                     /locus_tag="MIW95_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBY3152522.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            1078..2250
                     /locus_tag="MIW95_003218"
     CDS             1078..2250
                     /locus_tag="MIW95_003218"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBY3152523.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            2297..3109
                     /locus_tag="MIW95_003219"
     CDS             2297..3109
                     /locus_tag="MIW95_003219"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBY3152524.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            3313..4638
                     /locus_tag="MIW95_003220"
     CDS             3313..4638
                     /locus_tag="MIW95_003220"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBY3152525.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEVDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSIKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            4851..5930
                     /gene="mltG"
                     /locus_tag="MIW95_003221"
     CDS             4851..5930
                     /gene="mltG"
                     /locus_tag="MIW95_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBY3152526.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            6102..6776
                     /locus_tag="MIW95_003222"
     CDS             6102..6776
                     /locus_tag="MIW95_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBY3152527.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFASIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            6763..8010
                     /locus_tag="MIW95_003223"
     CDS             6763..8010
                     /locus_tag="MIW95_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBY3152528.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRAPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            8082..9746
                     /locus_tag="MIW95_003224"
     CDS             8082..9746
                     /locus_tag="MIW95_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBY3152529.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(9843..>10102)
                     /locus_tag="MIW95_003225"
     CDS             complement(9843..>10102)
                     /locus_tag="MIW95_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY3152530.1"
                     /translation="ILGTEANYVLDEVELKVQVGKLYEQLNKILTPREREIVQLRYGL
                     TPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
BASE COUNT         4125 a         1046 c         1853 g         3078 t
ORIGIN      
        1 taattaagag gtatatacta aatattataa ttataatagc actatcattg gggttgcaat
       61 taatattaaa tactgtaagt attggtatta ttaagttcct agctaaataa aggagtttat
      121 aatggatatt atagagggat atatagatta cataaaagat aaaaaaaaat tatcagagaa
      181 cacagtggca tcatacttta tggatataaa aaaatatatg gaatatataa atcaaaaaag
      241 cattaaacta gtagatatag tagaaaatga tattataagt tacttaataa agttggaaaa
      301 agataatgta tcaatagcta caatagcgag gatgatatcc tctataaaat cttttcatga
      361 ttatttattt ttaaatcata tatgtacaaa taatcctgca aaggatataa aaaaacctaa
      421 aataaaaaaa gaaaatataa atatacttac agaagaagaa attgaaagac tattaaattt
      481 tccaaagtta acaacaccta aattgattag ggacaaggct atttttgaag tattatatgg
      541 tactggtata aaagtatctg aattagtaga gatgaatata gaggatattg acctagatat
      601 tgattatata tattgtaatt catgttgtaa aaatcaaaga gtgataccac tttgtgatat
      661 tacaaaactt tacttagaaa agtatctaaa agaagcaaga cctaaaatgg ctgtagaagg
      721 tgaaaaatca ttattcgtaa gttcattagg tcaaaggttt acacgacaag gtctttggaa
      781 agtcataaaa aaatattcta atctagccaa tatagataaa aatataaatc ctacaatgct
      841 tagacattca tttgctattc atttgttaaa tgaaggtgct aacattgctg ttgtaagtaa
      901 aattttagga aatgttaatt tatctagttt gcaagtatat ttaaatcata tagataaaaa
      961 cgtgagaaga gaaataaagg aaaaacatcc tagaaatgat gttgatgtag aattacaaaa
     1021 agcagaagct aaatagacta tatagcaaat tttataattt aaaaggagta tatttatatg
     1081 agcagagtaa tttggattgt aattgatagt gttgggatag gagcattacc tgatgctgaa
     1141 aaatttggtg atagtaagga tgtaagtacc cttggaaata tatttaagga atatccagat
     1201 attcagattc ctaatatgag aaatttaggt ataggaaaca tagatggtat agattttttt
     1261 gaaagtataa aagaaccaat aggttgtttt ggaaagtgta aggaaatgtc tcagggaaaa
     1321 gatactacta caggtcattg ggaaatgact ggtataatag ttgacaaacc atttaagaca
     1381 tttgaacatg gattctctaa ggagataata gaagagtttg agaaaaaaac tggaagaaaa
     1441 gtagtaggaa ataaaccagc ttcaggaact gtaataatag atgagtatgg agagcatcaa
     1501 ataaaaactg gagatgtaat agtatataca tcagctgata gtgtatttca gattgctgct
     1561 aatgaagaag taattcccct agaagaactc tataatatgt gcaaaatcgc tagggaaata
     1621 atgatggggg ataatgcagt agctagagta attgctagac cattcatagg taagaaaaaa
     1681 ggtgagtttg tgaggacttc aaatagaaga gattattcac tagacccatt tgaaccaaca
     1741 gttcttgata atataaaaga atcaggactt gatgttttag cagttggaaa aatagaagat
     1801 atttttaatg gaaaaggaat tacagatgct atccacacta aaagcaatat ggatggagtg
     1861 gatgaaactt taaattatat gaaacaagat aataaaggtc taatatattc aaatctcgtt
     1921 gattttgatt ctaaatatgg tcatagaaga gacccagaag gatataagaa agctcttgaa
     1981 gagtttgata gtagacttcc tgaaataatg gctaatatga gagaagatga tattttaata
     2041 atcaatgcag accatggaaa tgacccaaca tataaaggta cagaccatac aagagaatat
     2101 atacctgtta tgatttatgg caataagatt aaaaaaggtt ttaatttagg agtaaaagat
     2161 acttttgctg atattggagc aactgttgca gatatactaa atgtaaagtt gcctaaacat
     2221 gggtcaagct ttaaagaaga tttattttaa actataaaat gataaaaact tagcttaata
     2281 ggatggagag attggaatgt ttgaaaagat agcacaaagt agtaaattta taaattctaa
     2341 aagtaatata aaacctaaga ttggtttgat attaggttct ggactaggtg atttagcaaa
     2401 tgatatagaa gaccctgtca caattaaata tagtgaaatt ccaaacttcc ctgtatcaac
     2461 agtagcagga catgctggac aacttgtaat tggaaaactt gaaggaaaag aagttattgc
     2521 tatgcaaggt agatttcatt actatgaagg atattctcag aaagaagcta cttttccagt
     2581 aagggttatg aaagaattag gagtcgagat tcttatagta actaacgctg ctggaggcgt
     2641 aaataaagaa tttaaagcag gagatttgat gcttataaga gaccatataa actttagtgg
     2701 aagtaatcca ttagtaggaa aaaatgatga cagatttggt gctagatttc cagatatgtc
     2761 agatgcatat tcttctaaat atgtagatgt agttaaaaac tgcgctaaag aatgtaatat
     2821 agatgtcaaa gaaggcgttt atatgttttt tagtggtcca aactatgaaa caccagcaga
     2881 agtgagaatg gcacaaattt tgggagctga tgctgtaggt atgtctactg ttccagaagt
     2941 catagtagct tctcactcca atataggagt tattggtata tcttgtataa caaatatggc
     3001 tgctggcatc ttaaatcaac cactaagtca tgaggaagta atagaaacta ctcaaaaagt
     3061 taaatcagaa tttatgaatt tagttaaaac aacggttaaa aatttataag ttaaagctta
     3121 ttatatattt tttgaggaag tttataataa aatagttaat gtttaatttg aaaagaatta
     3181 ataaaaagct gtcttgatat agaaagttat ttctaataaa ggacagtttt tttataatac
     3241 tacaacattt gaaagtgttt taaagtttaa tatttattta aattattata gattagaatg
     3301 gagatttcaa ttatgagaat gtatgatatt ataaaaaaaa agagagataa cttagagctt
     3361 agtaaaaaag agataaacta ctttatagaa aactattcaa agggagatat acctgactat
     3421 caagcttctg cactattaat gtcgatttat ttaaataaga tgaataaaca ggaaactgta
     3481 catcttacag aagcaatgat gaactctgga gacatgataa acctgtctga ggtagatgga
     3541 ataaaggtag ataagcatag tactggtggt gttggagata agactaccat agctcttata
     3601 cctttggttg cttcatgtgg agcaccagtt gcaaagatgt caggtagagg tcttggtcat
     3661 actggtggta ctattgataa attggagtct ataccaggtt tttcgacaga aatggagata
     3721 agtaaattta ttgattctgt aaataagtct aaaatagcag tttgtggtca aagtgcaaag
     3781 gtagcaattg cagataagaa aatatatgct cttagagatg ttacagcaac tgttgataat
     3841 atatcattga tatcatcaag tgtaatgtct aaaaagttag cttcaggagc tgatgcaatt
     3901 gtgttagatg taaaaactgg tgatggagca tttatgaaga cattagaaga gtcttttgag
     3961 ttagctaaat ctatggttga tataggtact ggtatgaata gagatacaat agggattgta
     4021 acagatatgg atgaaccttt aggttttgca gtgggaaact ctttagaagt tatagaagca
     4081 attgagacat taaaaggaaa tggccctaaa gaccttgttg agctatgtga agtattagga
     4141 gcatatatgt tggttttatc aaaggtagca catgattttg aagatggtgt tgaaaagata
     4201 agagaatcaa taaaatctgg tactgctata caaaaactca aagagtttat agagaatcaa
     4261 ggtggtaata aagatgtagt agatgattat tctttattca aaaaatcaaa gtataaatat
     4321 ccagttaaat caattaaatc aggttatata agtaaaatta aagctgaaga tgttggtgta
     4381 tcagctatga ttcttggagc aggtagagaa actaaagatg atattttaga tttatcagca
     4441 gggataattt tagaaaagaa agtaggagat tatgtaaatg aaggtgaagt tttagcttac
     4501 atgtattata atgatgaaca aaagctttca ttggcaaagg gaagatttat tgattcctac
     4561 tctattgtag attcaaaaat agaaaaaaat aaattaatat atggtattgt aactaaagaa
     4621 gagattaaaa agttttagaa aattttagaa aagtgattat aaaaggccta acatgtcaaa
     4681 aataatgtta ggttttttat tttttgataa actataaatt ataccaaaaa tatatatttt
     4741 aaaacatttt ttacaatata aataagttta ttctacaaga tgattgaaaa ttaaagtatc
     4801 tagttttata atagaaaatg gataaatata cataaggagt agattatatt atgaacttta
     4861 aagaaaatag attgaaaata gcagtattaa taattgttat tttgattatc ttagcaggta
     4921 tatttgtgtt tatacaaata ggtccttatg ataaaaataa caaaaaagat gtaattattg
     4981 acgttccaag tggagcatct gttggaaaaa tatctgacat attatatgaa aataaattaa
     5041 taaagaatga actattattt aaacttttgg taaaggttag caataaagct ccgtctataa
     5101 aatcaggaac atacttactt aatcaatcgt attcaaacaa tgatataata agtcttttag
     5161 tttcaggtaa aatatatcag gatggtataa aagtgactat tccagaggga gctacatcta
     5221 aagaaattat agcaatgtta gtaagtaaaa atttaggaga taaagctact tttgaaaatt
     5281 taataaaaaa acctcaagaa ttttatgata aatttccata tttgaaggaa gatggaataa
     5341 cctctttaga agggttcttg tatccagaaa cttattattt taattcaaag aaacaatcag
     5401 aagaagatat tttaagcgaa atgttaaaag tatttgacag taaatacacg gacaagttta
     5461 aaaagaaaca aaaagaatta aatatgactc ttcaagaagt aatggaaatg gcatcaataa
     5521 ttgagaaaga agctgttctt gacaaagaca gacctataat agcaagtgtg ttttataata
     5581 gacttaaggt aggtatgcct cttcaatcag atgctacaat acaatatata tttgaagaga
     5641 gaaagaaaat tgtaacatat gatgatttaa aaattgattc accatataat agttataaaa
     5701 ataagggatt acctcctaca ccaatatcaa atcctggtat aaaatctatt gaagcagcat
     5761 tatatccaga gaaaacagac tatttatatt ttgtagctaa gattgatggt ggaaataatt
     5821 atagtacaaa ctatcaagac catttaaaat acgttaaaga atataaagaa gcaagggata
     5881 aacaatcaaa agatacaaaa gcaacaaata aagaaaatac aaaaaaataa accatgatta
     5941 taaaaagtct aacagggtaa gaataatgtt gggctttttt attgaaaatt gttattctta
     6001 gatttataat aaagaaagtg taaatatata taacaatatg aatttaaaaa ataataaatt
     6061 aatataaaac ttgaatattt attattttag ggagtgtttt tatgagtaat atagttaatg
     6121 atttggttga agattatata agagtgacat taaaagaaaa agaaggattt ttaaaagatt
     6181 tagaaattta tgcagaggaa aatagtgttc ctataataca taaagaagtt tcagatttat
     6241 taaaagtctt gttaaaggtt caaaaaccaa agagaatttt agaagtagga tgtgcaattg
     6301 gttattcttc tatttttttt gcaagtatta taggaaaaga tgcagatata ataactgttg
     6361 agagaaatga aaaaatgata gaaaaagcaa aagaaaacat aaaattagct ggatttgaca
     6421 ataacataac tatactagaa ggagatgcag aagaaaaact tagtcaagtc caaggtgaat
     6481 ttgaccttat atttatagat gcagccaaag gtcaatataa gctatttttt gatttagtta
     6541 tagataaatt aaaagatggg ggacttttag tatctgataa tattttgtat aaaggtatgg
     6601 ttgctcatga tgattttgtt gtaagaagaa aaaagactat agttaaaaga atgagaaatt
     6661 atttagatta tatatgtaat tgtgactatt taagcacatc attggtacca ataggtgatg
     6721 gtgtagcact aagttataaa gaaagtaaaa gaggtgatgt agatggaatt aaataaagta
     6781 gaacttttag ctccagcagg tgatttggag agattgaaaa tagctataac atatggagca
     6841 gatgccgttt atataggtgg agaaattttt ggaatgaggt ctgcagcgaa gaattttagt
     6901 aaggaagata tggcagaggg agtagctttt gcacatgaaa gaggcaagaa agtattcgta
     6961 acagtaaata taatacctca taatgaagat tttctacaat tagaagacta tttgttggaa
     7021 ctagaagaga taggaataga tgcagttata atagctgacc caggagtgct tagtgtcatt
     7081 aaaaaagtaa ttccgaatat ggaaatacat ttaagtacac aagcaaatac aaccaattat
     7141 ctaagtgcaa acttctggta cgagcatgga ataaaaagag ttgtagtggc aagagaatta
     7201 tcttttgatg aaatatctga gataagagca aaaacacctt tagatatgga tatagaagcg
     7261 tttatgcatg gtgctatgtg tatttcttat tctggtagat gtttaataag caactatatg
     7321 actggaagag atgcaaacaa aggttcttgt gctcaatctt gtagatggca atatcatttg
     7381 gtggaagaaa aaagacctgg tgagtatttt ccaatatacg aagatgaaag gggtacattc
     7441 ttctttaact caaaagattt atgtatgata gagtatatac cagagttaat caaatctggt
     7501 ataacaagtt taaaaataga aggtagaatg aagactgcat actatgtagc tactgttgta
     7561 agagcataca gaatggcaat agatgaattt tacagagccc cagaaaactg gaagttcaat
     7621 ccaatgtgga tggaagaact aaaaaaaggt agtcatagac attttacatc aggtttttac
     7681 ttgaataaac caacaacaga agaccaaaat tatcaatcag catcatatgt tagaaactat
     7741 gattttatag gtatagttag agaaacagaa gatgaagatg gtcttattgt tgttgagcaa
     7801 agaaataaaa tgtgtgttgg tgatgaaata gaagttatgg gaccatataa agaaactatg
     7861 tttactaaaa ttgaagaaat gtacaatgaa gaaggagaag ctatagaatc agctcctcat
     7921 cctagacaaa tagtgaagtt aaagctatca gtaaaagttg gtaaagatta tatgcttaga
     7981 aaagttatag aagaaaaggt tgaagaataa taaaaaattg ataaaaatag tattagcccc
     8041 ttagaaattg tcaataatga aattatctaa ggggtgattt catgtcaaaa aagaagactc
     8101 catttttgaa aaaagtggga aagagaagct ggtgtatatt tactataatt ttaataattt
     8161 atagtgtttt aatttatagg ctagttgata tacaagtatt aaaaggtgat aaatacaaac
     8221 aaagtgttga atcacaaagc gttgagaaag tcgagttaaa tagtggaaga ggaataatct
     8281 atgatagaaa caacaagaag cttacagata caagtaaatc tcaggtatta attgttgaaa
     8341 aagaaaaatt aaataataac tataaaattt tggaacttat taagaaagct actaagatga
     8401 atgatttaga tatatataaa gctgtacaag agcaattgac acgccctata atacagattc
     8461 agactaaaaa tatcgataag agtatgaaaa aagaacttga gaaaaatgga ataatggtag
     8521 aagaaaaaac tatgaggtac gcaaaagatg gtttattatc acatacaata ggatatataa
     8581 aagaagatga taaatcaggt caatcaggaa ttgaaaagag tatggatagt gtacttagaa
     8641 attcaaatga aaagtatata agtgctttta aagctggaga tgcaggaaat gaaaaatctc
     8701 taaacatact aaagggaagt gtaaaaactg ttgataataa ggataaagat agacatctaa
     8761 aaactactat tgattataac attcaaaaaa aattagaaca aatactaaat aaagaagaaa
     8821 atcctacagc agcaataatc tcggaagcat ctacaggtga aattcttgca atgtgctcta
     8881 gacctaattt tgaccaaaat gacatatcaa aaagtttaaa aggaaaaaat ggggaatttg
     8941 aaaatagagt tataaaagcg acatatccac cagggtctgt atttaagatg gtagttttat
     9001 tttctgcact tgaaaatggt gttatagatg agaactatac ttacaactgt acaggtaaga
     9061 ctaaagttgg gaatactaat gaaatactgc gatgtaataa aagagatggt catggtttcc
     9121 aaaacttaag acaagctttt tctaattcat gcaatcctgc atttttagat atagctatga
     9181 aacttggaaa agaaaagata ctcaaatctg ctgaaaaatt acatttattt gaaaaggttg
     9241 atattggtct tgatgaagaa aaaattagag aagctccaaa aaacatatca ataagaaatt
     9301 tagctatagg tcaagaaaat atagaattta caccacttca aataaatcaa atgactcaaa
     9361 taatagctaa taatggaaca ttcaaaccac tatacttata taaaagtctt gtggacaata
     9421 atatgaatac aataaaaact tataagtctt caaaaaaaga agaacttatt tctccttatg
     9481 tgtgtacaca agttaaagaa tatatgaagt ctgtatctag gattggtaca gctaaagacc
     9541 taaaagatat tgaaggtggt tgtggagtta aaactggtac agctcaaagt agtttaaata
     9601 aaaaagctat agaccacgga tggataacag gattttatcc tgaagaaaga cctaagtatg
     9661 taataactgt gttagtagaa ggaactcaaa aaggaaataa atctgcaaca cctatattta
     9721 aggaaatatg tgaaagtata aaataaatta tgtactataa aatttaagga tatatcgtaa
     9781 ctttgatttt agatagaaaa ctttgtctaa tgatgtacga tatatccttt taaaatatag
     9841 tattaacttt cttgaacaag ctctttttct agttttttaa gagctttttt ttcgattcta
     9901 gatacatatg accttgatat atcgagtttt tgtgcaattt ctctttgtgt cttatagcca
     9961 taaggagtta acccatatct taactggaca atttctcttt ctctaggagt aagaattttg
    10021 tttagttgtt cgtaaagttt tccaacttga acttttagtt caacttcatc aagaacatag
    10081 ttagcctctg tgccaagtat at
//
LOCUS       DAGQWR010000148        10048 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.149, whole genome shotgun sequence.
ACCESSION   DAGQWR010000148 DAGQWR010000000
VERSION     DAGQWR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10048
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.149"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            160..1311
                     /locus_tag="MIW95_003226"
     CDS             160..1311
                     /locus_tag="MIW95_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3152531.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDDDLDKMLELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TESLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1429..2067
                     /locus_tag="MIW95_003227"
     CDS             1429..2067
                     /locus_tag="MIW95_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBY3152532.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            2068..3090
                     /locus_tag="MIW95_003228"
     CDS             2068..3090
                     /locus_tag="MIW95_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBY3152533.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEYIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRINYKELDR
                     IDIDEL"
     gene            3080..3700
                     /locus_tag="MIW95_003229"
     CDS             3080..3700
                     /locus_tag="MIW95_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152534.1"
                     /translation="MNYNSNKNKYLLKDINLEDIFIDKNIVLKFLGYGNRKAPDTISE
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGIALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3824..4510)
                     /locus_tag="MIW95_003230"
     CDS             complement(3824..4510)
                     /locus_tag="MIW95_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBY3152535.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGNSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4524..5069)
                     /locus_tag="MIW95_003231"
     CDS             complement(4524..5069)
                     /locus_tag="MIW95_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3152536.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCIDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5297..6544
                     /locus_tag="MIW95_003232"
     CDS             5297..6544
                     /locus_tag="MIW95_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBY3152537.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6631..8625
                     /locus_tag="MIW95_003233"
     CDS             6631..8625
                     /locus_tag="MIW95_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBY3152538.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIKNTFSENTN
                     KTSEIEIYNKILKINTFPVEYKKNNTEYIVVLIKDITNEKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            8625..9947
                     /locus_tag="MIW95_003234"
     CDS             8625..9947
                     /locus_tag="MIW95_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBY3152539.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
BASE COUNT         4082 a          986 c         1670 g         3310 t
ORIGIN      
        1 aagtttaact ataaattagg tttatatttt ataaaaattt gttaaaaaaa acaattaaaa
       61 attgtaaaga aaaaagatat aattagaact gtagtaataa atgttttatt atagataata
      121 gagttttaag tatcttaaat aatatttgga ggaatatgaa tggaattaag accaagaagt
      181 gcaaaattag aagaatttga taaagtaata gaattaataa actatgtatt tagaatatca
      241 agaaatcata agccaactat gatggaagaa tttcctcttt tactaagtaa aaataatata
      301 gaaaatatga ttataatatc agaagatgat aaagtggttt ctgatgtaaa ctatctaatt
      361 caagatgtat ctattcaagg aaatagattg aaggttgcag ctataggtgg agtatgtact
      421 catccagatt atgaaaagag aggctattca tctaagatat tggataaagt agaagaaaaa
      481 atgttttatg atggtgtaga tattgtaatt atatcaggaa caagaagtct atacagtaga
      541 agaaattgta gtttggtaaa gagtttttat aaatatacaa taaaacctga agatgtaaaa
      601 atagcatatg aaatagttga atttgatgaa actaattttg aaaaagatga tgatttagat
      661 aaaatgcttg aactatataa tcaaaatagt actagattta taagaacgag agatgagttt
      721 caaaaactat tgcatgcagc aactattgcc tggggaccaa ttggttacaa gaaggtattt
      781 ataaaggaaa ataataatat aataggatat ttgataataa gaacaataaa aaaagaagat
      841 tctactgtag gagaagttgc tgaaataggg ttaaactctg taaatgttga aaatatattg
      901 aaatatgttg caaataaatt tggattagaa tatttaaatt ataaagtaca tgtgaaaaat
      961 cttaaagacc agttgaaatg taatgggaca gaatctttgg actatcaaca aggaactatg
     1021 aaaataataa attttactaa gttgtgtgat agtttaagaa gttattttag tcaatatgta
     1081 gattttgaat tactaaaata catggaattt aaacaagttg aaaataaata tattattaaa
     1141 tacaaagaag aagaacttgt aatagaaaat ttggataagc ttaataaact gttttttgaa
     1201 aaaaatgagg aacagtataa tgaatttaaa catctaaaaa atatatatga atttgctaca
     1261 aaggcatttc ctgtagattt tccatggact gcaaatttaa attatcaata gttttagatg
     1321 atattaaata aaattgattg atacctagag ttagtccttg ttaaataaga gaaattattg
     1381 gatattgaaa aagcaatttt gtaagtaatt agaaaggatg agtattatat ggacatactt
     1441 gatgatatag cagaatgtat attaaatatg ggtgtagata atatagagaa tttagtaaaa
     1501 attgctatag ataaaaatat tgatgtggaa gatatttatg aatatggttt aaacaaaggt
     1561 atgataggag ctttagataa gtttgagaat aaggaatact atctttcaga agttatagta
     1621 tgcacagatg cattaaataa aggtattaat cttttgaagg gaactggaaa agtaaagaaa
     1681 aaatcaaagg gagtaattct gatgtctgta gtagaaggag atactcatga gataggaaaa
     1741 aatattgtaa aagtaatggt agaagccact ggatataagg ttattgactt aggtgtaaat
     1801 agaaaaagtg aagatataat agaagaagct ataaaaaaca atgtagatat aataggctta
     1861 tcttcaatga tgacaactac aatggaaaat atgaaatcgg ttatagacga gcttaatatg
     1921 attgagatag ataaaagacc aaaagtaata attggtggag gacctgtaag tatggagttt
     1981 gcagaagaaa taggagcaga tggatacagt tcaaatgctc caaaagcagt taaattaata
     2041 aacaaactta taggaggtga agtttaaatg ttttcagata tgactttact tcagtatata
     2101 aatagtgaag atagaggttt ttttctgcct gatatgggta caaatggatt gttcttaata
     2161 aataaaaagg catatgaagt atatgatagt gtagatttgc aattagaatt agcaaagact
     2221 atggacaagt attttgaatc agattttata tactcattct gtgatgggat tacattttgt
     2281 gaaactctgg gtttagagac attaagacca gattatgatt ttccaagtgt attgacacat
     2341 actataactg acagagataa attaagaaaa ttagatgttc cagaccctta tacgagtgga
     2401 cgtatgccac tcaatataga gagcttatca gttatatcaa aaagtattaa aaaaccacta
     2461 tatgaatcga tacaaggtcc atttacactt gcaggtcaat tagctggagc tactcaactt
     2521 ttaagatgta taataactga taaaaaattt gtagaagaat tgttggaatt tacaactgaa
     2581 ttggtaagaa gatatgctgt agctgcaaat aaagcaggag caaaatatat ttcaatagca
     2641 gagccaacgt cagtaaccct aagtaaagat agatttgatg aatatatagt aaaaaattta
     2701 aataaaatct acaatgaact tgattgttgg aaaggtatgc atatatgtgg agacaccaga
     2761 gaattattag ataatatgct aagttgtaat atagatgcag taagtttaga ccaaatttta
     2821 gactatgaag aaattgctcc aatgattcct agagatatag tgttgattgg taatttagac
     2881 ccaataaaac ttcttggacg ttctaagcca gataagatta gaagagagac actaaaattg
     2941 ttaaagaaga tgagaggata tgataacttt ttatgtgatt ttggctgtaa ctgcttaaat
     3001 accactccag tagaaaattt acaagcagct ataaaagcag gaagaattaa ttataaagaa
     3061 ttagatagga ttgatattga tgaattataa tagtaataaa aataaatatc ttttaaagga
     3121 tataaattta gaggatattt ttatagataa gaatattgtg ctaaagtttt tggggtatgg
     3181 aaatagaaaa gctccagata caattagtga aatcgttgaa agagaaataa gaaatataaa
     3241 tgatatttta gatatcaaga tatatataag tgaagttgat attaataaaa cacctaaaga
     3301 atgtaaaaaa gcatttgctg tactatatac aataggggat aaaattgata ttaagatgaa
     3361 tgactatatg gaaaattgca atatgatggc agggatagca ttggataaaa taggtatagt
     3421 atgtttagat tatattaatg agaaaataaa aatgtattta aaaggaaagt atgatagtct
     3481 taaaatatcg catgaaatat atccaggaga taaagatttt gaagtagaga gacaaaagga
     3541 tatttataac tatgtaaaaa ataaatataa tagtatagaa atagaaatca atgattatca
     3601 tcaactatcc cccataaaaa gtgttgcaat gcttatttta atgggagatg aagaaaattt
     3661 agaatcaaga tgtagcaaat gtcctagaaa atgcttttaa ctagttttca agactaagat
     3721 ttgttttgtg tatgaattgc aataattttt ttggtcattt tatgtataaa ctaaaaaaat
     3781 tattgcaaag taatattcta tttaatttga acagacttac aaactatagt agtttcttta
     3841 attcaataag tgtatttatt tcataagttg gtatatgatt agtataattt atactgttat
     3901 tggaatttaa ccaacaagta tctattcctg aatttattcc acctaagaca tcagaggtta
     3961 agctattccc aatcattaag gcagaagatt tatcatgaca attaaacttt ttgagagcgt
     4021 atttaaatat ttcaggattt ggttttgata ttttcaactc ttcagataca accattccat
     4081 caatatatcc ttttattttt gaattttcaa gtcttttctt ttgaactgca gaaattccat
     4141 ttgttattat aacaatagta tacttttttt taagatattg taatatatcg attgcacctg
     4201 gaattaaaaa tgtacattca cctaaaaaat ttaagtacat tttgctaaga gtttcagaat
     4261 caacatccaa agatatttta tttgcaaaaa gttcaaatct aagtaattta agttcattca
     4321 aagttataat atttttttca aggtcgagcc aaagcttatc acttatattt ctatatgact
     4381 ctatataatt ctcaaaatta aattctaaat caaactcaga tagaagtttt ttaaaagcat
     4441 gtgattcact tttcttaaaa tcaaataaag tatcatctgc atcaaaaaat ataaatttat
     4501 atgtcttcat ggtttttact cctttacaag atgactgcaa aaattacttt actttcacta
     4561 tttgagtcat tttgccattt atgctttgtt cctaaaggaa cttttacact atctcctttg
     4621 gacaaagtaa attcatcatc atccataaaa agtttaactt ctccctccaa aacatatgca
     4681 atttcatctc cattatgagt aatcctatca atacaagaag aagtatttgg agctaaatcc
     4741 ataattgcaa attctaaatt accactagag tctggaatta aaatctcata tataacttct
     4801 ttgcttccag gtaaaataac tttttttcta ttatcatgtc ttactatcaa gtttttttta
     4861 tctacttcag atacaaaaaa tgtaaataaa ggaacgttta gagaagaggc tatagatttt
     4921 aatgaattta cagatggatt agcaagacct cgttctattt ggctcaataa tgatggtgta
     4981 acatcagcta gttttgctaa atctcgaata cttaagtttt gtttttttcg aacttcagct
     5041 attttttccc ctaaatttaa atcattcata taaatatcct ctctttttaa tttaaaaaat
     5101 attaacttaa aataatcaag taaaatgatt atgtagtatg aaattattat aacatataga
     5161 attaggtttt gaaaaaacta tgttcaatat aattaaataa attaaaatat aattgacaaa
     5221 gtggatacag aatacttaaa atatatttaa ataaaattaa gcaaaagata gttaatttat
     5281 gataggaggg gacattatgc aaataacgac ttttggagct atatttggtt tattaatagc
     5341 aattatttta attataaaaa aatttcaagc tgtatatagt ttaatgttag gagcatttat
     5401 tggaggatta gttggaggag caaatataac acaaactgta gattttatgg caaatggagc
     5461 tatgaatata tcaccatcaa ttcttagagc tttggcatca ggtgtattag ctggaagttt
     5521 aataaaaaca ggagcagtag ataaaatatc agaacagatt gtaaaaatat ttggtgaaaa
     5581 gagggcttta ttttcgattg ccatttcaac tatggtactt gctggagtag gcgtaaattt
     5641 ggatgtatct ataataactg tagcacctat aggattatat ataggtagaa aattaaatta
     5701 ttctaaattg tcaatactat tagctatgtt gggagggggt aaagcaggta atataatatc
     5761 accaaatcca aatactatag cagtagcaga taatttttct gtcaatctct ctagtgttat
     5821 gatggcaaat ataattccag caattattgg agttgttatt acagttattt tagcaagtat
     5881 attaataaat aaaggaaata aagtacaatc atatgaaatt ttagagcaaa gagaagattt
     5941 accaagttta tttaaatcat tatgtggtcc aattatagct atatttctat tgtttttagg
     6001 aaatgtatct cctatagtta tagacccaat gatagccctt ccaataggag gaatcgtaac
     6061 tctaattgtc acaggaaact tgaacaattc aagagaatat ttagcttttg gattatctaa
     6121 gatgcaaggg gtatgcatat tattacttgg aacagggact atcgcaggaa ttatacagat
     6181 gtctgaatta caacagagta caataggtgc tttacaattt ttaaatatgc cacaattttt
     6241 attggcacca gtttcgggta tacttatgtc attagcaaca gcatcatcaa cagctggtgc
     6301 tactatagca tcatctactt ttcatgatgc tataataaat ggtgggctat caccgatttc
     6361 aggtgcatca atagtaaatg ctgggtctag tgtattcgag caattaccac atggctcact
     6421 atttcatact agttcaggta gtataaacat ggatataggt gaaagattta agttaattcc
     6481 atatgaagca ttgataggaa tagtaatgac tataatttca acatctattc aattagtttt
     6541 ataataaata taaatattta atgaaaaaag taggattttg ttattaaata ttgaatttat
     6601 tatagtaaaa aagaagattg gagttttaaa gtgaataaaa aaaagattgt tattataggg
     6661 attatttatt catttttagt agtattttca cttacaaata tgtatgtaaa tatggagtat
     6721 aatttaaatg tatttgaata tattaagaag tcacttccat ttacagaaga agaaaaaaag
     6781 tggttagaaa aacacaagaa tttgatttac agctctgacc aaagttcacc tcctttgaga
     6841 tataagggta aggaagatgg acagtataag ggaattattg ttgatttaat aaattctctg
     6901 tcaattcaaa ttggtagaga tttttatttt aaaccaaata attggtggaa agaatctttc
     6961 gtaaattcaa tagatgatag tataaagttt tttgacttga ttccatcaaa agaaagggca
     7021 aataaattta tatttactga cccaatatac actttgagtg caaatatatt gaaagataaa
     7081 aagtcccaag atataaatag ttacatggat ttaaagggga aaacagtagc aattccagaa
     7141 ggtgattatt ctataaattt tttaaagcaa aaaattcaag atataaacat tttattgact
     7201 ccagatataa agactggagt caaccattta atgtctggaa aagtagacgc agttgttggt
     7261 gatgaaccag tattgcgata ctatataaat aattatggac tttcaaataa atacagtgtt
     7321 ttaagtaatc ctatatatac taaaaaagca gttcttgctg tacctaaaca atatgaagag
     7381 ctggttagta ttttaaataa aggcattttt aaacttcaga aaaatggagt gtataaagat
     7441 ttaaagaaaa aatggtattc tacgtataat gaagttgatg atatattgta tgagcgagga
     7501 attgtgccta gtatttatct ttttataggt ataattttaa tatcaattta tgttttttat
     7561 agctatacat atttgttaaa gatagaaatc aaaaggaaaa cagaacaggt aatagaaaat
     7621 aaaaaaactt tagaggcaac ttttaatagt attacagata taataatgct tgtagatgaa
     7681 aataacaata tagtagaatc gaacaaagta ctatatgact ttatgggtga aatgagttat
     7741 aagatagcag atttgattag catgataaaa ggtgttatta aaaatacctt ctctgaaaat
     7801 actaataaaa ctagtgaaat agaaatatac aataaaattt taaagataaa cacttttcct
     7861 gtagagtata aaaaaaataa tactgagtat atagtagttc ttattaaaga tataactaat
     7921 gaaaaaatag tagaggcgaa attacttaga gaaaacaaaa tgatatctat aggacaatta
     7981 gcttctgggg tagcacatga gataagaaat cctttgggta taataagaaa taattgctat
     8041 ctattaaaag ataacgttac aatggaagaa gtgaatgatt gtgttaaatc aatagaaagt
     8101 aatgtagata gggcaagtaa tataataacc aacttgttaa atttcgcaag aatatcagat
     8161 gataatttag aacatataaa tataagaaat tttattgaaa atatagtgaa acttcaatat
     8221 aaaatgttac agttaaagaa tgttgaaata aaaattgact gtgaacataa tttagtatgc
     8281 tatataaatg gagaatcgtt aaaacatgta tttataaatt taatttctaa ttcaatagat
     8341 gcaatacatc aagatggaaa aattattata tactgttatg aaaaaaatca ttgtttattt
     8401 atagatttta aagataatgg tgaaggaata aaagaagatg ccctaaagga catatttaat
     8461 cccttctata caacaaaacc aattggtgag ggaactggtt taggtttata tattacttat
     8521 aatgagatta aaaagaataa tggagatata agtgttgaaa gtaaattggg ggttggaact
     8581 tgttttcata taaaaattcc attaaataaa gaggtgacta tataatgaaa atactattag
     8641 ttgatgatga gctagaatat ggagtggtaa tgaaaaaaat tctacaaaag aagggctatc
     8701 ttgtagatat agcactaagt ggtgaagaag ctataaatat aattaaaaaa gataaaaatt
     8761 atgatttggt tttatctgat gttatgatga aaaatatgga tggagtacag ctattagata
     8821 gaattaaatc tattaataga gatatagagg ttatattagt tacaggatat ggaagtattg
     8881 aaaatgcagt tgatgctatg aaaagaggag ctttatctta ttttataaaa agtaatccca
     8941 ttgaaaattt attagaagaa gttgaaaagg taaaggcaag taaaatatct gtttctgtac
     9001 aaaaaaataa ttttgaattt actttggaat ctaaaaatag agattttaat aatatcataa
     9061 aaatagcaaa aaaagctgct tgtaaagatg ttaatatatt aatcttgggg gaatctggtg
     9121 taggaaaaga tatattagct agatatatac atagtataag tccaagaaag aatgagatat
     9181 ttgtacctgt aaattgttgt tctttttcag aaaatctatt ggagtctgag ttgtttggtc
     9241 atgaaaaagg ttcttttaca ggagcagtag atagtagaaa aggtagattt gaactttcga
     9301 ataaaggaac tctatttttg gatgaaatag gagatatacc tctaaatgtt caggtaaaac
     9361 ttttgaggac acttgaggac aaatctatag agaggatagg aagtaataaa tctattaaag
     9421 ttgactttag gttaatttgt gctatgaata aagaaccaaa agtggaaata tcaaatggaa
     9481 atataagaga agattttttt tatagaataa gtacaataac aataactata ccaccactaa
     9541 gaaagagaag agaagactta actactctta tagaattttt cttaaataag taccaaattg
     9601 aacatgataa aaagatacac tcgattgata aggaagtaaa ggatttttta ttaaattaca
     9661 attatccagg aaatataaga gaattaaaaa atataataaa tagacttgta gttttaagtg
     9721 aagaaggcaa tttatcaaaa gacaatttaa atttaatatc aaataatgta tacatagatg
     9781 ataaaataag tattagacct ttaagagaaa taagaaaaga gtttgaatgt gagtatattg
     9841 aaaaagtttt atctttgtgt ggaaataata tttcaaatac agctaaaaaa ttggagatta
     9901 gtagaagaca gttaaccaat aaaatatcag agtataatat taagtgagaa aaaaacttct
     9961 cattaagtga gaaaaaaatt tctcaaactg tattaaaaga taaagagaaa aaatctgttt
    10021 atcttttttt attataattt taaaaatt
//
LOCUS       DAGQWR010000149         9520 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.150, whole genome shotgun sequence.
ACCESSION   DAGQWR010000149 DAGQWR010000000
VERSION     DAGQWR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9520
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.150"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..596)
                     /locus_tag="MIW95_003235"
     CDS             complement(<1..596)
                     /locus_tag="MIW95_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBY3152540.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWE"
     gene            817..1641
                     /gene="pdaA"
                     /locus_tag="MIW95_003236"
     CDS             817..1641
                     /gene="pdaA"
                     /locus_tag="MIW95_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBY3152541.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            1830..2594
                     /locus_tag="MIW95_003237"
     CDS             1830..2594
                     /locus_tag="MIW95_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3152542.1"
                     /translation="MTTICVMIDHIFLDTEYRQPTIHKHLAKHIIISQSGNLSVTFDK
                     GESLQCKGIIIDSNVSHTVSSKDNKMLVFLIDDTSILAQNISQHQLLNHSFFVIDNLI
                     VERIQNIYTACQVKGLSQSYIDFSKQAFRLLNLNYHISSITDSRIQWALNHILKSTHI
                     HNEIFFEICAEVKLSKSRFSHLFKEQMGVSLNSYLALSKLRKAYELLLKSGDITSAAM
                     DAGFSSPSHFSASSKKYLGITASELIGKCKIYFIRQ"
     gene            2697..3875
                     /locus_tag="MIW95_003238"
     CDS             2697..3875
                     /locus_tag="MIW95_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBY3152543.1"
                     /translation="MKTNKISFNKGLYYLNTNANFNFQLNRIIMWDGGRLEDIQKVSS
                     KIVDSESWKTELMYLAQEAEQDGRTENAIAYYRMSEFFMYDGDPDKISVYKKATTLFY
                     NYYSEYFSNGTVQIFHVPYENITLPVMYAKAQGEVKDVILLHGGNDSYFEEFFFPMLY
                     LASQGFDIYLFEGPGQGGVMRLQGKHFTYQWERPVKTILDYFHLENITIVGASLGGFL
                     APRAAAFEKRIERVVAWSVFPNFLDIIVGSQPIKMQNLLRLLLKLKTKWLVNFIIKQK
                     MKSGDSMVVWGLKHGMYAYEASTPYEYFQKIKYYQIDSIAKNINQDILILGATQDHFI
                     DYRTVGKEIDLLNNVRSLTFRLMTEAEQASNHCNCGNSKLVLDTISNWIMMMKIHDRV
                     "
     gene            complement(4246..5031)
                     /locus_tag="MIW95_003239"
     CDS             complement(4246..5031)
                     /locus_tag="MIW95_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3152544.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFDIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            5496..5861
                     /locus_tag="MIW95_003240"
     CDS             5496..5861
                     /locus_tag="MIW95_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3152545.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            5939..6493
                     /locus_tag="MIW95_003241"
     CDS             5939..6493
                     /locus_tag="MIW95_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBY3152546.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            6713..7387
                     /locus_tag="MIW95_003242"
     CDS             6713..7387
                     /locus_tag="MIW95_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBY3152547.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      7742..7838
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            7892..8068
                     /locus_tag="MIW95_003243"
     CDS             7892..8068
                     /locus_tag="MIW95_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152548.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(8256..8495)
                     /locus_tag="MIW95_003244"
     CDS             complement(8256..8495)
                     /locus_tag="MIW95_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152549.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(8640..9449)
                     /locus_tag="MIW95_003245"
     CDS             complement(8640..9449)
                     /locus_tag="MIW95_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY3152550.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT         3423 a         1412 c         1181 g         3504 t
ORIGIN      
        1 cattcccatg ttggaagatt aaatttttta agatatgaat ttattttaaa actcaatggc
       61 tccaaagtag ttttttcata gttattaaat tttactaaaa ggcaatttac aatatttatt
      121 atatcaatag catctgataa agctctattt tccactttag aattacaatt tgttaaagta
      181 ttttttaagt attctaagct ataatcttta tgatatggtt ctaaaataac tgctaattca
      241 attaaatcaa taaatttgtt ttttaacttt ggcatatgat actcaaaaaa cttttttttc
      301 agacttaaat catgacatat aatgaaacta tcttctacaa aatttacaag tctatcttcc
      361 accagttcta tttcattagc tatttctaaa tcatttttat ctaaattttt acatactgag
      421 aatacttcta taggtacctc aaatttgttt tttattaatg attcataggt atatattttc
      481 cagtctttaa ccttgacagc tccaacttct aaaatttctg aatttaaaca atctaatcca
      541 ctaacttcta tatctaaaaa aacaacatca ttcaatacac ttattatatt atccattttt
      601 atatcccttc aagttaaacc agcctaactt atttacaatc atttctcttt atattttatc
      661 atttatatac actttattta agataattaa tatcaacttt atatcttaca gtaaacttct
      721 ttcatttatt atttgtatat gtgaatattt ttaactataa atattcaaac tatattataa
      781 gaaatatact ttaaatttta aataagaggt gaaattgtga aaaaagatag cctaaaaaaa
      841 tacattatga taggagcatt tgctctaata ctttttggaa ttgctagtat aaattttaaa
      901 tcattagata aaacaaaaac tcaaatttct agtccaacac tcgacacaca tgaatatgat
      961 tggtatttta accctaggga ggatggtaaa cagccatcac ctataaaaga agctgatttt
     1021 tttaaaaaat acggagctta ttatgtagga aatcccaatg aaaaagttat atatctatca
     1081 tttgatgctg gatatgaaag tggaaacact ccaaaattat tagatacgct taaaaaacac
     1141 aatgctaaag ctcaattttt cgtagttgaa agttatataa aaagcaatcc agagttaata
     1201 aagcgtatgg aaaaagaagg acatttagtt tgtaatcatt caaaaagcca tccatcaatg
     1261 gcaggtatta cagattttga aaaattcaaa gaagaaatta caagtgtaga aaaagcatac
     1321 aaagatgtta ctggaaaaga aatgcctaag tattttagac ctccaatggg taaatttagc
     1381 gaacaatctt taaaatatac acaagattta ggatataaat caatattttg gagttttgct
     1441 tatgtagatt ggtatgaaaa gaaacaacct actcatgaat ttgccaaaaa caaaatttat
     1501 tcaagaactc atcctggtgc aatagtttta ctgcatccaa attcatcaac aaatactgag
     1561 atattagatg aggttcttac acattgggaa aaagaaggtt ataaacttaa aacacttgat
     1621 tatttaaata ataaaaaata gtatcataaa ttttaaaaaa ctctatcatg aatcatagta
     1681 aaaatatagt tatttataaa tactttaatt ctcgatagag ttttttattt ttcattgcct
     1741 aaaataggat ttttaaatac ttgtaatcac taagctttct tgttaaaaac tgtataatat
     1801 gctaagctat gaacaaggag ttgacagcta tgactaccat ttgtgtaatg attgaccata
     1861 tatttttaga tacagagtat agacaaccaa ctatccataa acatcttgca aaacatatta
     1921 ttatatcaca gtctggtaac ttatctgtga cgtttgataa gggcgaatca ttacaatgta
     1981 aaggaattat tattgattcc aatgtatcgc atacggtgtc aagtaaagat aataaaatgt
     2041 tggttttctt gatagatgat acaagcatac tcgcccaaaa tattagtcaa caccaattac
     2101 taaatcactc ttttttcgtt atagataatc ttatagttga acgaatacaa aatatttata
     2161 cagcttgtca ggtaaaagga ttgtcccaaa gctatattga tttttcaaaa caagctttcc
     2221 gtctattgaa tttaaattat catatttcgt ctattacaga cagtagaata caatgggcat
     2281 tgaatcatat tctaaagtcc acacacattc acaatgaaat cttttttgag atatgtgccg
     2341 aagtaaaatt gtccaaaagc cggttttctc atcttttcaa agaacaaatg ggagtatcac
     2401 taaacagcta tttggcttta tcaaaacttc gcaaggctta tgagttattg ttaaaaagtg
     2461 gagatattac ctcagctgct atggacgcag ggtttagttc accgtctcat ttctcagctt
     2521 ccagcaaaaa atatcttggc attactgcaa gtgagcttat aggaaaatgt aaaatatatt
     2581 ttatccgcca atagcgtcta tttgaaagtc agtacttcta atatttgtta caataaatgc
     2641 atagcattaa agattactct ttattgctat gcattatctt tttaggagtg atgacattga
     2701 aaacaaataa aatttctttt aataaaggtc tatattacct taatacaaat gccaatttta
     2761 attttcagtt aaacaggatt attatgtggg atggggggcg cttggaagat atacaaaaag
     2821 tttcctctaa aattgtcgat agcgaaagtt ggaaaacaga acttatgtat ctggcacaag
     2881 aagctgaaca agatggacgc acagaaaatg caattgctta ctatcgtatg agtgaatttt
     2941 ttatgtatga tggtgaccct gataaaatat cagtctacaa aaaagcaact actctattct
     3001 acaactacta ctctgaatat ttctcaaatg gaactgtaca aatttttcat gtaccatatg
     3061 aaaatatcac cctacctgta atgtatgcaa aagcgcaagg cgaggtaaaa gatgttatat
     3121 tgctccacgg tggaaatgat tcttactttg aagaattctt tttcccaatg ctttatcttg
     3181 cgtcacaggg atttgatatt tatctttttg agggacctgg tcaaggtgga gtgatgcgtt
     3241 tgcagggaaa acactttact tatcaatggg aacgtcccgt taagacaatt ttagattatt
     3301 ttcatttaga gaatataact attgtaggtg cttccttggg aggttttctt gctccacgag
     3361 cagcagcctt cgaaaaacga attgaacgcg tggtagcttg gtctgtattc cccaatttct
     3421 tagacataat agttggttca caaccaataa aaatgcaaaa tctacttaga ctactgctaa
     3481 aactcaaaac aaaatggcta gtcaatttta taataaagca aaaaatgaaa agtggcgata
     3541 gtatggtggt atggggttta aaacatggaa tgtacgcata tgaagcgtct acaccctatg
     3601 aatattttca gaaaatcaag tattatcaaa tagattctat tgcaaaaaat ataaaccaag
     3661 acattttgat tttaggtgca acacaagatc attttattga ttatagaact gttggtaaag
     3721 agattgattt actcaacaat gtccgatccc ttacatttag gcttatgaca gaagcagaac
     3781 aagctagtaa ccactgtaac tgtggaaata gcaaactggt tttagataca atttcaaatt
     3841 ggattatgat gatgaaaata catgatagag tctaaatatt gatttttaaa ttatacaaaa
     3901 tccatctgct agcatatcct tattgctttg ttcaaataat cacttgttaa aaaattggtt
     3961 cccacttacg aaatactacg tttatagaaa tgaactatat catgtaagca cacaatatat
     4021 atgataataa gacccaatta ctcaatgtaa ctgggtcttt cttatgtcat agattttata
     4081 ggactcatga aatgtaaaag agtatttcac cgatttccaa agggaacttt acttagataa
     4141 ggagaccata aatgctaaca aacccatcta ctagacacct ttcaagtatt tgagcgatta
     4201 tacaatacac gttaagaatt acacgaaaat ttattatttt tatagctatt taaaaagaat
     4261 tgtccattca tttatctctt tgtatcctat attatgatat attttacctg ctacatcatt
     4321 attataaaaa agacatggaa cttttccttc caacaataat tctttactca aattgtaaac
     4381 catataactt gcaagtcctt ttcccctata tcttttatct gtacttacag agaccaccat
     4441 tgccaaaaaa ctagtttcca taccagttga cacagtgcta atcatagtat catcatttct
     4501 aataaaatat gctcttacat ttccctcttt taaatgttct ttcctttctt ctatgtaatt
     4561 tttacttact ttatagtctg acctatttaa aagttttcct atttcatcaa tatctttttc
     4621 ctgagcattt tctattttat actccttcat gttggaaaaa tctatttctt ttatttcttt
     4681 taatacacaa aatttattat ctagtttttc ataaaaatct ttgtatttac ttattagtct
     4741 atctattaca catttttttc cacttatgct ttctacatct aaatctttta tatgattctt
     4801 catttctcct atatcaaaat tatttttaaa actataaatt ataagtgttg acttattttt
     4861 taatattata gaggttatat ttccatcttc tacctgagac catatatctt gatattgaga
     4921 gctaaatcca taattctcaa cgtatccaat tatgaataaa tttaaacttt cttcatgtga
     4981 tacatattgc caaaattctt tttctaaagt attatctatt tttacaatca agcagatatc
     5041 ctccttctat gttaataatg atatacctta taatttatag tatcatttaa aatcattata
     5101 ttctaccaaa tattctaaat ttttaaatat ctatgcaata tgttcgtatt tatttttata
     5161 ttctgttact gcttcactcc aagcttcttc tactgaaata cctctcttat ttgcaatttt
     5221 ttgtgctaat tctattattt tcatagcttt tcttgatata ttcatatata ttcctcctct
     5281 aaattatcat aagttatttt aaagttaaat ttctatagtc atgttatata tttatttact
     5341 tatatcttac taattattta tgtactaaat atgaactaca atagaagaaa tttagaactt
     5401 aataaaacat aaaatttata tacagttcat atatatttgt catatcacaa gacaagtgat
     5461 ataatttatt atcattttac tgggggtgat atgctatgaa aataagagct gaatatacat
     5521 gtcctcttga aattgtacat gacataatca aaggaaaatg gaaaactatc cttatatttg
     5581 aattaagaca atctaataaa acattttctg aattgcaaca taatatttat ggaataaatc
     5641 aaaaaatgtt gattcaacaa ttaaaagagt tacgtgattt tgggattatt gataaaataa
     5701 gttctgctgg atatccatta catgttgaat attttcttac taatcgtggt aaaaaaatgt
     5761 taaaagcagt ggaaataatg caagaaatag gaatcgaata tatgcttgaa catggtcaac
     5821 aagaaattct agataataaa ggaatttgct acaataagta atcctataca taccaaaaag
     5881 taagtaattt acatttttta caatagtatt ataataaaag taaatagaag gtataatcat
     5941 gtctatagct ttaatattga ttgatattca aaatgattat tttaaaaatg gaaaatgtga
     6001 attatttcaa tcagaagaaa ctgctgaaaa tgctaaaaaa atactactgt tttttagaaa
     6061 aaacaattta ccagttattc atatacagca tatatcaaca gataaaacag cttctttttt
     6121 tcttcctaat actcatggtt cagaaataaa taaaattgtt tttccactag ataatgaaga
     6181 aattataatt aaacacacac cagatagttt ctttgaaaca gatttgcaaa gtatccttga
     6241 gaaaaaaaat attactaatc tagtaatctg tggaatgatg agccatatgt gtattgatac
     6301 aagtgtaaga acagcaaaga aacttcgata cgacataact ctaataagcg atgcatgtac
     6361 aacgaagaat ttatcttgga ataatataga aattaatgca aatactgtac atcaagtatt
     6421 catggcatca ctacagaaca gttttgcaga tgtaaaaaac acagataaat ttttatctaa
     6481 ctatcacaaa taaaaacata cactatgtta aaagagcaag tttgatttat ttaagcttgc
     6541 tctttaatca tttaaaatta gatgctttct atcattttta tatacataat atacgtttat
     6601 acattttata ttcatatata actattatat aaattttaaa ctttactata ttcacataaa
     6661 aaatgtagta ttatagtata tataatatta tatagaaggg ggtgctagat aaatgaacca
     6721 tcatatattg attcaatttg aatatatagc aagaatcatt actgctggtt tctgtggggc
     6781 tttaattgga tatgagagaa agaatagact aaaagaagct ggaatacgca ctcattttat
     6841 agtggcatta gcttcagcac ttataatcat tgtttccaag tatggattta atgatatagt
     6901 gggtactcca ggtattggtc tagacccagc ccgcgttgca gcacaagttg tcagtggtat
     6961 aggtttttta ggtgctggat taatatttat tagaaatcaa tcagtaagtg gtcttactac
     7021 agctgctggt atatgggcaa cagccggtac aggtcttgcc attggctctg gattatactt
     7081 agttggtatt gtttcaagta tcattattgt tattgtacag ataacacttc atagagatag
     7141 gaagtggatg aaactcccta cagcagagca agtttctctt aaaatttcta acagcactga
     7201 ttccatagaa tatattgaag gtaaatttaa aaaatctaat attgaaatta taaacatgaa
     7261 tattaaacgc cttgatgggg attggttaga agtagttata tatactaagt tacctaaagg
     7321 atataaaaaa acaaatttat taaaattatt tagcgataat cctcttatag aaactttaga
     7381 aatataatat aaaaattgaa gcttaaaaat aataatatat ttaatcaagg tcaatatata
     7441 aataattacg ggatgagtct atataaaatt catcccttgt tttttgccat tcatatatat
     7501 tttttcaaga tatttttgct catctattat ctagccaagt aactcttgtg aactaaactt
     7561 tttaatacaa aaacttcaat acaaaactaa atataatttt tattaaatat tctacatgta
     7621 tagtttgttt cttgagtgaa aaatagattt catattgtta agatactgtt aaatatttca
     7681 attttctgac aagtatgttg aacaataact tatcctagtg tattatttta gttgtagaat
     7741 aaaactaaat cggcaaaact agagaaattt agtgacgcaa agctataggg actaacgctt
     7801 ataaaataaa ctatgagtta tgtcagccag ttgccaaaaa gatattgttc tttttgtttt
     7861 atgaaagttt tttaggggga tagtaatata tatgtttaga gatgaaatgg ataaatgtac
     7921 acacatgtta actgcttata ttagtagttt atatgattat tgtgatttta tagatacaca
     7981 gctagatgat tttatactag agtacggaga aaatgtagta gaatcttgtt tacatcaagt
     8041 gatggtattg gtaagtaagt ataattaaat agaaattgtt ttctagagaa ccctttttag
     8101 ttttatctac atttaaaaat gttattatat taaaaaacta atctccccta tatgcaaagt
     8161 tttcttccat ataacaattt cttcccattt atactttttc acttgtatct ttaataaaat
     8221 tcaacttcaa atattaatat acttcaaaaa ttattctatt tcttatcttt acaccaattc
     8281 ctatcagtat tatcctttat ataggttcta actatttcgc cacaatctaa acataaatct
     8341 gaatatatag tagattgtac tggaaaagtt ttatttataa ctttatctaa ttttccatct
     8401 cctctatatt ttaatccaat agtttcagca tatatgccac cagttgttaa aatacccttt
     8461 tcaatattta cacttccaca attagaacat ttcacattta taacccccta aacaattatt
     8521 ttttcatgct atcttataat ttaaaatata atttgctacc gattttataa cacagtagca
     8581 aatcggtggc aaatcaacat tttttcattc tatttaccat ctaatccatt gaaatatggc
     8641 tatttagtta taggaacttg aatttccgtt acaaactctt ctacaattcc agtctcatgt
     8701 atatctatct tatagatttc tatagggtca gcaattatct tataattatt ttcttctata
     8761 aatttaaaca ccttctccat atacatctta ttattagaat tactcccagt ataatttaaa
     8821 gttacataat atccttcatc aaaaactata ttatatactt tctcattact ttccagaaaa
     8881 caaaatacag atttaaactc attaaatata cctttattaa ttgcttcagt agaaaaggca
     8941 gaaccaatat tgttgtttcc aagtatatta aatctatctt catattcttt ctgaagtttc
     9001 tgaattaaaa aatcgaagtc ttcatccctc tttatatcag catttaattc taaagctttt
     9061 cttttttcta tatatacaac ctctatctca tctatttttg tattttgaat tacactttca
     9121 atcgaactca atctttttat aatattttcc ttatggctta taaattcagc tattttttta
     9181 tcaattaaat cgagttcttc atttaaaata tttttcgtag actctatact tctgtcatct
     9241 aggtattctt ttattttctt cattggaaag ttcaagcttc taaattcttt tattaaattt
     9301 aatttccata tatctgatat attatacatt ctatatccat ttatatctct aactggtctt
     9361 agaatgccta tttcttcata atacattaat gaatctctac ctattccata tattttagat
     9421 atctctccta ttttatagta atctttcatt tatataataa actcctccga aatcctattt
     9481 ttattttaaa tcaacattat tactctataa tactataata
//
LOCUS       DAGQWR010000150         9429 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.151, whole genome shotgun sequence.
ACCESSION   DAGQWR010000150 DAGQWR010000000
VERSION     DAGQWR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.151"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            234..1862
                     /gene="aspD"
                     /locus_tag="MIW95_003246"
     CDS             234..1862
                     /gene="aspD"
                     /locus_tag="MIW95_003246"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBY3152551.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDEI
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1983..2978
                     /locus_tag="MIW95_003247"
     CDS             1983..2978
                     /locus_tag="MIW95_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBY3152552.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGIGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3136..3804)
                     /locus_tag="MIW95_003248"
     CDS             complement(3136..3804)
                     /locus_tag="MIW95_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBY3152553.1"
                     /translation="METVSNNQSSVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(3903..4215)
                     /locus_tag="MIW95_003249"
                     /pseudo
     CDS             complement(3903..4215)
                     /locus_tag="MIW95_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431045.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4642..7062)
                     /locus_tag="MIW95_003250"
     CDS             complement(4642..7062)
                     /locus_tag="MIW95_003250"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBY3152554.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(7416..7766)
                     /gene="rsfS"
                     /locus_tag="MIW95_003251"
     CDS             complement(7416..7766)
                     /gene="rsfS"
                     /locus_tag="MIW95_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBY3152555.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(7883..8455)
                     /gene="yqeK"
                     /locus_tag="MIW95_003252"
     CDS             complement(7883..8455)
                     /gene="yqeK"
                     /locus_tag="MIW95_003252"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBY3152556.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSRNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(8456..9145)
                     /locus_tag="MIW95_003253"
     CDS             complement(8456..9145)
                     /locus_tag="MIW95_003253"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBY3152557.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         3122 a         1561 c         1238 g         3508 t
ORIGIN      
        1 ataaaaaaac taaggtgata attaaattga atatttattt tatataacaa tcatttttta
       61 ttaaaaggta ttttttattt taacatatat ttaataaggc taaatagagt tattatttta
      121 atttcatact ttactttttc tataaaccta ttataattaa agtataatca tttttgcaac
      181 ttgtttattt tattataaaa atgtgaaact tattacataa ggaggaattt aacatgaaaa
      241 atcatactta taaagaaatc aaaaacatat atggaaaaat aagccctttt gaatttaagg
      301 acaaattaat agatattgca aaatacactg caaaagaaaa taacagagaa ttacttgatg
      361 caggtcgtgg aaatccaaac tggacttgtt caactgctag agaagccttt tttacttttg
      421 gtcatttcgc tattactgaa actcgttcta attgggattt aggtcatttg gctggaatgc
      481 ctcaaaaaaa gggtattaaa gaaagatttt ttaaatttat aaatgaaaat atagatatgc
      541 ctggagcata tttagctaga gatattataa actttggtat aaatgaactt ggatttgatg
      601 gagatgaatt tgttcatgaa ctagctgatg gaattattgg tgataactat ccacttccag
      661 atagaatgtt acctcatatg gaaaaaatag tacacgatta cctagttcaa gaaatgaaat
      721 atgatatatc aggtaaatat ggtgatgtag aaatttttgc tgttgaaggt gctacagctg
      781 ctatgtgcta tatatttgat tcattaatgg caaatgaact tctaaaaaaa ggagatacaa
      841 tagccttaat gactccaata tttacacctt atcttgaaat acctaacctt cctcgttacg
      901 actttaaagt ggtaaatata aatgctaatg aagttgatga aaaaggtgct catacatggc
      961 aatatacaaa aaaagaatta gagaaacttc gtgacaaatc tattaaagct ttattcgttg
     1021 ttaatcctaa taatcctgct tctatagcta tggatgaaat atcttgtaat aatctaatag
     1081 atgtaattga aaattataat aaagatttaa tgataatatc agatgatgta tatggaactt
     1141 ttgtagaaga tttttcatcc ttaatgtcaa aactacctta caacactgtt ggagtatatt
     1201 cttattcaaa atattttgga gttacaggat ggagacttgg tacatttgcc cttcataaga
     1261 aaaatgtttt tgataagaaa ataaatgatt taactggaga attaaaaaaa tcagtagata
     1321 agcgttacag tgatatgagt cttaaccctt ctagtctttc atttatggaa agagttgttg
     1381 ctgatagcag acttgttgct ctcaaccata ctgctggtct ttctactcca cagcaagtac
     1441 aaatggcgtt tttctctgct tttgcattga ttgacaaagt agatgcctac aaaaaactca
     1501 atatgagtat ctgccataga cgtcaaaaac tattatttga agctttagag cttccaatta
     1561 atgaaaacaa aaacaatgct gcatattaca ctcaatttga tattgaagaa tgggctaaat
     1621 taaactacgg agaagactta tttaaattta taagtaaaac tgcatctcca gtagatgttc
     1681 tttataaatt agctaatgac tattcagtag ttttacttag tggaaatgga ttctatggcc
     1741 ctgaatggtc aattagaata tctctagcaa atctatatga tgaagcttat actaagatag
     1801 gaaaagccat aagagagata ttaaatggtt atatcgttga atggcaaaaa attaagaaat
     1861 aattatatta cactgaaata tctaaattat atgaataaaa gagtttatgt agaccttaac
     1921 tacttttttt gatataatcc tatcggaatt ataaatttta tttatgaaag gcagagaatc
     1981 gtatgagtat taagaaaaaa gttgccatag tttttactgg tggaactata tctatgacag
     2041 tagatgataa agtcggagca gctataccaa ctttatcagg tgaacaaata ctatcaatgg
     2101 ttaccaacat tgataaagtt gcagatgttg aagtttttaa ttttgatgaa attccaggac
     2161 ctcacataac accttataga atgatggaat tgaaaaatta tgtaaatgat ttattagcaa
     2221 gaaaagatat tacaggtgtt gtaataacac atggtacaga tagtttagag gaaacagctt
     2281 attttctaga ccttacaatt gacaacatca aacctgtaat agtcacaggt gctatgagaa
     2341 gtagttctga acttggatat gatggttcaa gtaatttatc tgcatcagtt tgtactgcag
     2401 tatccaaaga tgctatggga aaaggagtcc ttgtagtttt aaacaatgaa gtattattag
     2461 cttctgaagc tactaaaaca aatactcttt ctctaaatac atttaaatct ctaactagtg
     2521 gtccattagg aataattgac tgcaacgaac ttgtccttac aagagatatt gtaaatagaa
     2581 caattataga tactgataag gtagaatcta aagttgcctt atttaaagca tatgtaggag
     2641 aaaatgctga ctttataaga tttgctgttg atagtggtta taaaggtata gttattgagg
     2701 caatgggtat aggtaatatt cctcctcaaa tgctttctgg agttgaatat gctagagaaa
     2761 aaggacttcc tgtagttata gtttctagat gtcattcagg tagagtattc gatagttatg
     2821 gttactttgg ttctggtaga gatttaaaaa atcttggttg tatatttggt ggggacttgc
     2881 caggtcaaaa agctagaata aaacttatct tagcacttgg aaaaactgat aaccttgatg
     2941 aaatcaaaga tttctttgaa aaaggaatct attgctaatt ctaataactt ctatttaatt
     3001 atttagttca gtttacaaga aaatacacac aaaccataaa atgagatgtt gacaacataa
     3061 cagacagtat gctcctgtaa aaggtaacat actatctgtt atattgtctg gattcccaaa
     3121 ttagctattt ggaacttaac taaaaaatcc taatataagg gcaactatta taatggcaat
     3181 cggaatacat actgtgtaat cctgttccat agagtcatca tctgaatgta gttttacaat
     3241 tcgcaattta gcaatcagtt tgaagcagaa atagcctatt attgttccaa ttacattcgt
     3301 gagtaagtcg tctatatcgg ttgctctgtg taacgtaaaa agctgaatag tttcgataaa
     3361 gagagataat ccaaaaccaa tcaagaatgt atttctcaac cgttcaaagg ttttgctgat
     3421 aagcggacat aaaaaaccta atggaacaaa aagtaaaata ttcaaaataa aactcaaact
     3481 aaatccatca ctaaaaggaa taagatttat gtttggatta aagaacgttt cccctaactg
     3541 tgacagtctt atatattcac tgaatgtagg gataccaaca atatgcgata gtgttaaaca
     3601 taagtagtaa tacattgcca gtgataaaat agatgttttt acctttggaa tatcactatt
     3661 tttcttgaat attacaacta agaaaatacc aatcaagata attcccaaca agaaaatagg
     3721 attaattaac aataaatctt tgatagcttc aaacagactg agttgaattt ctacacttga
     3781 ttgattattt gatacagtct ccatttgtac acaccccctc ataacatttc aattatttct
     3841 aaaaaacaaa atagaagata aatcttaaaa tcatcctatc aaaattttta aatttgcctt
     3901 tatcagttta taccaccagt taattcctgt cttacttctg tatggtattc ttgggattct
     3961 ttcatcaaat tagacaaaaa aatggttacc atatcaataa actggttacg ctccatatct
     4021 cctgaagcaa acatcccctg agaataaatc cgtatcagga atctccacat ctactgcacc
     4081 tatcacaagt catttgataa catcctcaaa caatcccaac ttggatatat cagcaacacc
     4141 cattagaata ctactgctga ccatccttgt tttcctggtg ataattttaa tcaactggga
     4201 agttgaaacg cctatctttt ttgcaagttc ctttttagaa acatggtagt tattatatga
     4261 atctgaatct cgttgtctgg gtgtttaaga taaagcatag atacgcacct tttttgcctg
     4321 ttttcattat ataaaaaaaa ttttaaaaat acaataatta tatttttttc gtttaagctt
     4381 agagcagttc cttgatgaac aacactttag aaactgaata atttacatac cacttaatac
     4441 attaacacga tttaacatta attataataa tatttaaaac aacaattaga gtgtataaga
     4501 atgaactttt tagttcttta atcatttttt tgtataaaat caaatctatt tttgtaattt
     4561 caacaatgaa aaaaggctta tctctatttt gagacagcct catttatttt ataaatttat
     4621 atttttgttc atatttttga gttatttaac aactatattt actaattttt taggtacagc
     4681 tacaacttta actatagttt taccttcaac taatcctttt attttttcat cttctaaagc
     4741 taccttttcc atctcatctt tagatatatt tgagttaaca gttaattttc ctctaacttt
     4801 tccattaact tgtacaacaa cttctatttc atcttttact aaagcttttt cgtcatattt
     4861 aggccagtct atatcaaata cacttccttc tttacctatc atagtccata gttcttcacc
     4921 taaatgaggg gcaaatggtg caattatagt tactatagtt tgtacacctt cttttataac
     4981 tgcttcgttt atattatcta actctttata tttatacata tcatttatta attccataag
     5041 agctgatata gcagtattaa atccgaattt ttcacttaaa tcagcagtaa cttttttaag
     5101 tgtagagtgt atagtatatc tcatatcctt atcttgggaa tttaattctc caaactctac
     5161 atccttttta accacatctg ctaactcgtc aaccaatcta taaaccctat ttaagaatct
     5221 aaagcatcca tcaacacctt gctctgacca gtctaaatct ctttctggtg gtgctgcaaa
     5281 taatacgaaa agtcttgcag tatctgctcc atactcatct attatatcca taggagatac
     5341 tgtattccct ttagacttac tcattttaga accatccata agtaccattc cttgagtaag
     5401 taagttttta aatggctcat tgaagtctac cattcccata ctcttaaatg cctttacaaa
     5461 ccatcttgca taaagcaaat gcattatagc atgttctact ccacctatat attggtctac
     5521 tggatgccat ctattaacta attccttact aaatggttca ttttcattat tgctatccac
     5581 atatcttaag aaataccaag aagaatcaac gaatgtatcc atagtatcaa cttcccttct
     5641 agctggtttt ccacagtgag gacaagttgt tgacataaat tgctttgaag tcgtaagtgg
     5701 agattctccc tttccagtaa attcaacatc tgttggtaaa agaactggta aatcttcctt
     5761 tttaactgga actattccac attcatcaca ataaaccaca ggtataggac aaccccagta
     5821 tctttgtctt gaaactaacc agtctctaag tctataatta attgttctct ttcctctacc
     5881 ttctttttct aacttatcga ttattccttc aaatccttct gaagcatcta atccattaaa
     5941 ttcttctgaa tttatcattt tgttatcttc tgttattaca tcaattatgt ctaaattata
     6001 tttttcagca aaatctctat ctctttcatc atgtgctgga actgccatta tagcacccgt
     6061 tccatactca actaatacat agttagctat ccatagagga actttcttgc catttaaagg
     6121 atttataaca tatctaccta taaacattcc ttctttttct acatcagctg ctgttctttc
     6181 tatttctgtc atagtatgca ttttttgaac aaaagcttca acatctgctt catattcagt
     6241 ccctgcaacc aattctttaa ctaattcatg ctctggagca agtaccatat aagtaactcc
     6301 ataagttgtg tctggtctag ttgtaaatac tgtcatagat ttatcagttc catctatatc
     6361 aaaaactaaa tctgctcctg tacttttacc tatccaattt ttttgcattg ttttaacttt
     6421 ttctggccat ccatctaatg tatctaagtc attaagtaat tcttctgcaa attcagtagt
     6481 cttaaaatac cattgttcta aatctttctt taatactgtt gctttacatc tctcacatgc
     6541 tccttgaaca acttgctcat ttgcaagaac agtctcacaa gaaggacacc agttaacata
     6601 tgattttttc ttatatgcaa gcccatgctc taaaaacttc aaaaatattt cttgtgtaaa
     6661 tctatagtat tctggagttg aagttgcaac ttctttatcc caatcataac taatacctaa
     6721 taaatttaat tgctctttca tttcttctat attttccatt gtccatttat gcggatgtat
     6781 tccatgtttt atagctgcat tttcagctgg taatccaaaa gaatcccatc ccattggatg
     6841 aagtacatta tatccttcca tttttttgaa tcttgcgaca acatcaccta tagaataatt
     6901 tctaacatgt cccatgtgta tttttccaga tggataaggg aacatttcta atgtgtaata
     6961 attaggtttt tcagtttgtg cagtatccat tttatactga ttattatctt tccaaatttt
     7021 ttgccatttc gattctactt ctttaaaatt atatacgctc atattttgcc tcctattttt
     7081 aattgtgtat aactacctta tttagtatta tttacttata atttccaaat aattctttga
     7141 aaataaaaaa tcctcgtcac taaacttacg tttagggacg aggataaatc ttcgcggtac
     7201 caccctaatt agcctactac atatatagac tctcttagat agcttttaat ccagccatgg
     7261 agaatagatt ttaatctagt aagttcaaag ttatataata ttagttctca ccttccacta
     7321 actctctgaa aaaatataat cttttactgc tctagtctat tcaatttaat ataatttttg
     7381 aaattttcta attataataa catatattat catgtttatg ctaatgtgtc aatatctata
     7441 taaggtgcat ctttccataa tttttctaaa ttataaaacc ctctattttc ctcattaaat
     7501 acatgaacca ttatgtctcc ataatcaagg agtacccaag tttgagttcc ttgtccttct
     7561 ttacctcttg gctctaatcc tagttttgct aattcatctt ccacattgtc agcaatagct
     7621 tttacctgtc tctgagaaga tgctgtagta ataataaaat aatcacacaa actagaaact
     7681 tttcctatat ttattataac tgtatcttga cccagtttat cttctatggc atcatatgct
     7741 atttttgtca tttgttccac tgtcatataa aaattcctca ctttcctaat taaaatacac
     7801 ttagttattg taacatatta atacgatttt gtaacatagt caaatttaaa tttattaata
     7861 tgtttgcctt tatatagctt ttttataata acttttcttt aattagatag tttcttgcca
     7921 taactgttct tggatgtatc tcttcttttt tattgatgac aaacattaaa gtattattaa
     7981 aagaagttaa aagtgcttca tcaagcttcc ctccatatgc taattctctt aatgtatcta
     8041 caactggaaa ttttcttcct tcttcaatta aatcagctat gtaaattatt ttttctaaaa
     8101 gagacatgtc ctctctccct gttgtatgat atttgattgc atttataata tcttcatctt
     8161 ctacttcaaa ttcatactcg catatgtacg ccccaattac actatgagac aaggaacgat
     8221 ttccatcctc tattgggtct aaatatattt catacttatt tacataatcc tctatttcct
     8281 tgtcacttaa atactttgca caatcatgaa tcattcctgc taagtaagct ttttctttgt
     8341 cacatccata aatctcacat aatttttcag cacattttgc aacatttctt gaatggctaa
     8401 gcctccccac tggtagcatt tgatttagtc tttggttaat attttctaag ttcatttaat
     8461 catctccata ttgatatagt ttattttcat ataaataatt ttctacattt tcgggtacta
     8521 agtaacgtat tgacttgcct tcatttagct gttcccttat atatgtggac gatatatcca
     8581 gtgatggaac ataaactgtt ataatgtcag cattatactt cttagttagt ttttctatag
     8641 tctcttgtga ccttaacaaa ctaattcctg gtctagttgc ggctataaaa gtagctaact
     8701 caaagttttt ctttacatct ttccactctt caacactaca aattgcatct gcacctgtta
     8761 taaaataaat gtctgcattt ttgtattttt tctttaagta ttttaatgta tctattgtat
     8821 aagtcctttt ctttctttcg atttctactt ttgaaactaa aaaatcttcg ttgctttcag
     8881 ttgctagaag tgtcatatta tatctatcat acttatctgt agtgatacat aatttatgtg
     8941 gtggatttcc tgatggtata aagattattt tgtcaatgtc atacttgtct ctaataaatt
     9001 ctgctgttgc taaatgagca tagtgtatag ggtcaaaagt acctcccaat atacctattt
     9061 tgattttgcc tttatggcga ttaaatttct ctagtttttc agtatttttc aactctgcca
     9121 tcttaactag attttcactt ctcatatttt gtctcctttt aatgttataa tactattgta
     9181 ccattatttt ttatttctta aagcttataa tttgtttttt tgggtatata attatattta
     9241 tatgctaaaa aacactttaa aaaataatta tttaacatga ttctgcttta ctttttaata
     9301 aaaataactt tttactattt tgtcttaatt ttgataaaat ataacataca aatataagtt
     9361 ttttgttaaa ttttattagt tgcttttgtt aatataatta catataaaaa aggaggactt
     9421 atgggtata
//
LOCUS       DAGQWR010000151         9370 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.152, whole genome shotgun sequence.
ACCESSION   DAGQWR010000151 DAGQWR010000000
VERSION     DAGQWR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.152"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            447..1652
                     /locus_tag="MIW95_003254"
     CDS             447..1652
                     /locus_tag="MIW95_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBY3152558.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVGPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNVKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1723..1944)
                     /locus_tag="MIW95_003255"
     CDS             complement(1723..1944)
                     /locus_tag="MIW95_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152559.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2085..2684)
                     /gene="recR"
                     /locus_tag="MIW95_003256"
     CDS             complement(2085..2684)
                     /gene="recR"
                     /locus_tag="MIW95_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBY3152560.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2697..3047)
                     /locus_tag="MIW95_003257"
     CDS             complement(2697..3047)
                     /locus_tag="MIW95_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBY3152561.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3106..4743)
                     /gene="dnaX"
                     /locus_tag="MIW95_003258"
     CDS             complement(3106..4743)
                     /gene="dnaX"
                     /locus_tag="MIW95_003258"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBY3152562.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKYSIDENESK"
     gene            complement(4820..5084)
                     /gene="ffs"
                     /locus_tag="MIW95_003259"
     ncRNA           complement(4820..5084)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="MIW95_003259"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5122..5213)
                     /locus_tag="MIW95_003260"
     tRNA            complement(5122..5213)
                     /locus_tag="MIW95_003260"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5177..5179),aa:Ser,seq:gga)
     gene            complement(5214..5669)
                     /locus_tag="MIW95_003261"
     CDS             complement(5214..5669)
                     /locus_tag="MIW95_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBY3152563.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5989..7260)
                     /gene="serS"
                     /locus_tag="MIW95_003262"
     CDS             complement(5989..7260)
                     /gene="serS"
                     /locus_tag="MIW95_003262"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBY3152564.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKTRLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEYKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7385..7591)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7718..8599)
                     /locus_tag="MIW95_003263"
     CDS             complement(7718..8599)
                     /locus_tag="MIW95_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBY3152565.1"
                     /translation="MNFLSLAKAASTNSNITKRTLENMLNNFSDNIPNIIYAIIVFVI
                     GIYISKIVRKMVSRFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGVIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFINEKR
                     ILDDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENNIKVPFVP
                     SSLLFNNMSNSNTMDKR"
     gene            complement(8611..9120)
                     /locus_tag="MIW95_003264"
     CDS             complement(8611..9120)
                     /locus_tag="MIW95_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBY3152566.1"
                     /translation="MNSDERRSKLIDILKESKCPVKGGMLAEILNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHRNSKEIYDELKIIVDLGGIVKDVIVNH
                     PTYGQITAELNIHSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIETLKEETIEVITK
                     ELEDRGILS"
     gene            complement(9247..9363)
                     /gene="rrf"
                     /locus_tag="MIW95_003265"
     rRNA            complement(9247..9363)
                     /gene="rrf"
                     /locus_tag="MIW95_003265"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2984 a         1648 c         1009 g         3729 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttctttt tccttttttc
       61 aaggacaagt aatactatat catctttatc tattctagtc aatataattt tttacttttt
      121 ttaaattttt tatttatcat gtattcttta tatttaattt ttaaaactta atgctaagta
      181 aacctattta tttcataaaa ctaatatata ttctatgctc tattttcctt tttattctat
      241 atttaaattt tttatttaca agaatatttt taatataaca tatttgataa aattaattta
      301 catttctttc acttgtaatt tttgcaatct tgtattagat taatattaaa aataatttat
      361 caataacctt attcacaaag acagttttta tatatcaaaa tataatacaa tataaatact
      421 atgctacatt ttaggaggat tttattatga gcaaaaaagt attaataatg tctgcttcca
      481 ctggtggtgg gcataatagg gctgctctag ctattaaaga agaacttact tccaaaacac
      541 ttgatggaga gccaattgaa tgtgaaataa tagatagttt gaaattagtt aataatacaa
      601 tggataaaat aatttcaaga ggatacgaaa aatctgctat atatactccc aaagcctatg
      661 gaagtgtata tagattatct gaaacaaatc ttttgtcaaa aaatgaattt aaagataatc
      721 tactaataac attcatggct aaaaaattta aaaagcttat tagaagcgaa aaaccagact
      781 tgattatagg aacacatcct tttccaatga ttgctctaag cacattgaaa aaaaacttta
      841 atttacataa taatgaatca aatgcttaca cagagcattt ttataaacat tatacaaata
      901 ccattaatgt tcctccgctt atttctgtat taaccgatta tactactcat tctacatgga
      961 tacaaaatga aattgattac tatattgttg gtcatgaata tgtaaaagaa ttattggtgt
     1021 ttgatggagt tggaccgagt aaaattagaa catttggaat ccctgttgaa aaatcttttc
     1081 tctctcacag agataaagat attgttcttt ctgagttgaa tttatctcct gataaattaa
     1141 ctgttttact tatggggggt agctttgggg ctggaaatat aaaagagaca cttgatgaat
     1201 tactggatac tgatagagat tttcaaatac ttgtaattac tggtaagaat gaaagcctaa
     1261 aagaaaaaat cgaaaaaaaa cttatgtctc gttatcatga taaaaatgta tgtgttcttg
     1321 gatacactga taagatgaat gatattttag cttctataga tgtattagta agcaaacctg
     1381 gtggacttac tacaactgag gctcttttaa aagatgtacc tatgatagtt ccttattata
     1441 taccaggtca ggaagaagag aatcttgatt tcttatctaa ttgtggtgca gcacttagaa
     1501 ctacaaaaaa atataacttg tcaatattac taaaagttct aatagatgac cctagcagat
     1561 tagaaatgct aaaaagaaat gttaaatcaa taagaaagtc aaattcagcc caaaatatag
     1621 ctaatttaat tttaaatata atttctggtt gataaaataa ataaaatccc tatagtatat
     1681 atattatcta ttagggattt tttattttct aattatttaa tattaaattg gttcataaaa
     1741 gtcttcttca taatcatact gataattttt ataattattt ctaaattcag catttttatt
     1801 tcgtacattt tttttagttt ttttcttatt agaaaaactt atatcctcaa aatcatcata
     1861 tttaggttta tcttttgatt tcattttttt aactcttttt tctttcattg cattatgttc
     1921 ttctaagttc ataattggtt tcatgttgca cccctatcaa aatttttaaa tgatttatac
     1981 aacaaattat acaatacaga agaaatttat tcaataaatc taaatcaaaa ttaatttgaa
     2041 tttaactcaa aaaactacaa aaatcgaggt attaacctcg atttttatat ttctcttctg
     2101 ccttctagtg cttttgatat tgtgacctca tctgcatact ctaaatcgcc tccaacagga
     2161 agaccatgag ctatccgagt gactttgatt cccattggtt ttactaacct agcaatatac
     2221 atggctgtcg cttcaccttc aatagtagga tttgttgcca ttattatttc tttgacttcc
     2281 tgattgccaa gtctttggat aagttccttt atttttaaca tatctggtcc aattccatcc
     2341 attggagaaa ttactccatt taatacatga tactgtcctt taaactctct cgttctctcc
     2401 atagctgcta catctcttgg atcttccaca acacatataa cacttgaatc tctactttta
     2461 tttgaacaca tgctacatgg gtctgtatct gttatattac aacaaattga gcaatatctt
     2521 atttctcttt ttgcatctat aatcgcttta gataaagctt ccacatcatt agtattcata
     2581 ttaataatat gaaaagccaa tctttgagca gtctttcttc ctactccagg aagctttgaa
     2641 aattcctcta taagtctcgt tatgggacct gtataaactt gcataaatac cacctactag
     2701 aataacccag gtatattcat acctccagtt accttactca tttgacttgc ttgtatatca
     2761 tcaatatttc tcaatgcttg atttactgca cttaaaacta aatcttgtag catttctata
     2821 tcatctggat caactacttc tggcttgata gttatatcta taacttcttt tttaccatta
     2881 acttttacag taactgctcc tccaccaact gaagcttcta cttctttact ttctaactct
     2941 tgttgagctt tttgcatatt ttcttgcatt ttttgagcct gctttaacaa gttgttcata
     3001 tttccaccac caggcatcat tccgcctcca aaaccttttt tagccataaa cactattcct
     3061 cctaaactta ttttcatttt attattatat cataatagta ttttattatt tactctcatt
     3121 ttcatcaata ctatatttta tttctaatat ctcttcactc actatgttct ttaatatctc
     3181 ctcaccaatg tcctttttct ctttaacttc aagatttata tctttaactt ctgatgtcag
     3241 tactattttt acattaaaac ttctatttaa aacatctctt attgtttttt ctaaataatt
     3301 tattgtatct tgagagctca gtctagtttt tgcaaaatca aatttatcat caaatataac
     3361 atacaacata ttagaatata cattaaaact ttttacttca cttaagagtg cgtatattgg
     3421 cattttttta tcttttttaa tttgtttaag tatattcttc caagatgact ctataagtat
     3481 aacgtcctca ttttttacat cttcataaac tacattttct tgttttacat ctatttctct
     3541 atcgacctca tactcttttt ctttattatt cccaattttt tcagacttaa agttccccag
     3601 ttcaatcatt ttttctaagt tctcaactct ttttataaga gcttctttac tttcatcaaa
     3661 cattggttga gcaattttca ttatggttat ttccactaaa acccttggat ttgaagatga
     3721 ctttatatca tcctgagtta ttgataatat atttaaaatc cttattaaat catttatatc
     3781 tatattttta gactgaattt taagttgttc tatagtttct tctggtaatg aaattatttc
     3841 atccaattct gatgaaacct tacataccat taaatttcta aaatgatcaa ttaagtcatt
     3901 tataagattt cttatatctt tgccccataa gacaaattca tttaatatct caagagactt
     3961 tttagtgtcc tgctctacaa tacattgaga caattcaaat aattgttcta tatttacgct
     4021 tcccattaat tcaacaacat ctttataatc tatcttactt tctccaaaag acatacattg
     4081 gtctaatata ctaagtgcat ctctaagagc tccttgagaa tttctagcta ttaaattaag
     4141 agctttatca tctatatcaa ttccttcatc ttcacaaatc tttttcattc taagggtcat
     4201 atctttaact gtaactcttt taaaatcgta tctttggcat cttgataaaa tggttgcagg
     4261 tattttgtga ggctctgtag ttgctaatat aaaaattaca tatgatggag gttcctctaa
     4321 ggtctttaat aatgcattaa aagctccttg agacaacata tgaacttcat ctattatata
     4381 tactttatat ttagcattgg ctggagaata tttcacactt tctctaagct ctcttatatc
     4441 atctacacta ttatttgaag ctgcatctat ttctacaaca tccattgtat tatcttttaa
     4501 tacagattca catatctcac actcattaca cggttcttca tttattttat tcaaacagtt
     4561 tactgctctt gaaaaaatct ttgcagtaga tgttttacca gtacctcttg ttccacaaaa
     4621 caaataagca tgtcctatat tatcactata tatttgattt tttaaagtct taataatatg
     4681 atcttgacca ataacatctt caaatttttg tggtctatat gctctatata atgctttatg
     4741 catcttttta cctctcttta gcaaaattaa taaaaaaatc cctcattatc taagggatta
     4801 gtcttttata tattaaatgc tgtacaccat ctatcgactt aattccatag gcgttaccaa
     4861 aacagttagc tcaacttagg cacccccacg gcacacaaaa gtgatcactt accgctgctt
     4921 ccttccggat ctgacggggt tcatgagctt tcattgcgca ggacctaagc atcaacacca
     4981 cttatttagg gcggccctac agaaaataag cctttagatg gaattcaatc ctgctatagc
     5041 ggattgcagg ttacagggca ccgctacctc cccatctagt acagcaaaat tacaaccata
     5101 tttaattact ttcattgttt ctggcggaga gagtgggatt tgaacccacg atacagtttt
     5161 acccatatac tcgctttcca ggcgagcgcc ttcgaccact cgaccatctc tccttaatct
     5221 ctttttctca atgccttaaa aaattcttgc aatatgatcg agcattcttc ttgcaaaaca
     5281 tcaaaagcta cttttacatt attattatta aaataatcta atttaaactc atgctgtttt
     5341 tctatatatg aattttttac atgtcttgtc cctattataa gcttttttat tctagaatta
     5401 actattgcgc cactacacat tatgcaaggt tccattgtaa catataaatc acaatcagtc
     5461 aacctccaac cacctaattt ttctgatgct tgttttatcg ctaaaatttc tgcatgtgcc
     5521 gtagagtctt ttaacgtttc tgtaaggtta tgagctctag ctattatttg attatcttta
     5581 actataatag cacctattgg tgtttctttt ttattatatg ctttatatgc ttcttttaat
     5641 gcttctttca tataaaatga actttccata attcctccca aaacttataa caaacttatc
     5701 ttatcataat tcattttata attcaactta attttatttt gtttatttaa ttattactat
     5761 atatagcgat atttttttta attagttcta aaataatttt tagatattca tacaagagtg
     5821 ttttataaaa atatttagtg ttttatgatg aaaattctgc tgtgtaaatt aacataaaaa
     5881 aactatgaaa ttaatattta taataatttc atagtttttt tatttattgt ttactcataa
     5941 tatattctct tcatcaaagc atactctaca tatacaatta atataaattt attttataac
     6001 atcaacaccc atataaggtc ttaattcttc tggaactact acagaaccat ctgcctgctg
     6061 ataattttct aatatagcag ccaaacatct tcctatcgct aatccagaac catttaatgt
     6121 atgaacatat tctgcttttg attttttatc tcttttaaat cttattcctg ctcttcttgc
     6181 ttggaaatct tcaaaatttg aacaagaaga tatttctaca tatctattgt aacttggcat
     6241 ccaaacttct aaatcatatt taaatgcagc tgtaaatccc aaatctcctg tacatatttt
     6301 tacaactcta tatggaagac ctaacatttg taacatagtt tcagcatcat gagttaatga
     6361 ttctaattca ttataagaat cttctggttt aacaaattta actaattcaa ctttattaaa
     6421 ttgatgttgt ctaactaaac ctcttgtatc tcttcctgct gaacctgctt cagatctaaa
     6481 acaaggagta tatgcacagt atttaattgg aagctctgca acatctaata tttcattagc
     6541 atgtatatta gtaacaggaa cttcagctgt tggtattaag aaatactcta atccttctat
     6601 tttaaacata tcttcttcaa atttaggaag ttgaccagtc cctataaaac tatttctatt
     6661 ggccataaat ggaggtaaga cttctgtata gccatgctta gctgtatgag tatttaaaaa
     6721 gaaattcatt aaagatcttt ctaatctagc tcctaatccc ctatataaag taaaccttga
     6781 cccagttatt ttccctgctc tttcaaaatc taaaatacct agacctgtcc ctatatccca
     6841 atgcgcttta tactcaaaat caaatttagt aggttctccc caagttctta tttgaacatt
     6901 gtcttcatct gtttcacctt gtggtacctc tggatgagga acatttggta ttctcattaa
     6961 tgtatattcc atttttgctt ctacttcttt tactttctca tcaatacctt ttatcttatc
     7021 tgataactct ttcaatcttg tcttttcttc tgttacatct tttccttctt ttattaattg
     7081 aggtattttt ttagaattta catttaactc atttttcata acttcaactt cttgaagtat
     7141 ttctcttctt ttattatcta gttccactac agcatcaagg tcaaattctc tttcacctct
     7201 tctttccata gcttttttga tatcatctag attttctctt attcttttaa tatctaacat
     7261 attaattcct cctaataatt ttgataatat aaattataat tccaattaat ttacaagtat
     7321 tctagctgta aacttttaat ttaaacaaat ttgaaaaata aaaaaagacc tccgtttacc
     7381 ctataaaagg gacgaaaatc ttttatccgc gttgccaccc taattgatta tactaaaacg
     7441 tataatcctc tcagacctat aacgtaggta aaccgtctag ctttaactag aagctctaga
     7501 gtagattcaa aaaccattgt aatgagttct caccatccac tcattctctg aatacaatct
     7561 gttttttact attttccttc accgcttttt aactaattgt tttatttgat aatcattata
     7621 ttatatatat taacatcttt caatactatt attcaaattt ttttagaatt ttctttaatt
     7681 ttttatatta aattcacttt tttacttaat tgaaattcta tcttttatcc attgtatttg
     7741 aattgctcat attattaaat aataacgagg aaggaacaaa tggaactttt atattatttt
     7801 cgtcaaattt atttttgact ttttccatta catcaaagta aacatcccag taatcatcag
     7861 ttttaaccca tggtctagca actatttgca tagtgttgtt tcctatagag tttaaaccta
     7921 taattggttt tgggtcatcc aatatccttt tttcatttat aaaaatttca ttcaatatgg
     7981 acttaactac atcaatatca ctatcatatg aaatattata tgaaaaatct atccttcttt
     8041 tattttgatg tgtgtaatta attatattgt tacttgtcaa ttgaaagttt ggtataatta
     8101 tggttttatt atctggggtt ttaagaacag tactaaatat ttgtatatca gaaactgtgc
     8161 cttctacacc tgagccttga atataatcac ctatattaaa tggcttaaaa aataaaataa
     8221 tcatacctga acctaaattt gataatattt ctttaaatgc taaacctata ctaaatccag
     8281 cagctcctaa tacagctaca actgatgtag tttgtatccc tataacaccc aagcttatca
     8341 atgatataat agataacata gatatgtata ttccatatac aatgaaatta tttacacctt
     8401 tactcattcc atatttgatt aaaaaccttg aaaccatctt tcttactatc ttagatatat
     8461 agatacctat tacaaatacg attattgcat atataatatt tggtatatta tcactaaagt
     8521 tattaagcat attttctaaa gttcttttag ttatattact atttgttgaa gcagccttag
     8581 ctaatgataa aaaattcatt taactcctcc ttatgataat attcctctgt cctctaattc
     8641 ttttgttata acttctatag tctcttcttt taatgtctca attgtatgaa tatgactaca
     8701 ttcagttaga actgataact gtttaaattc atttgtctca accttcttca taaaattatc
     8761 tatatccatt ttagagtgaa tattaagctc tgcagttatc tgaccatatg ttggatgatt
     8821 tacgatgaca tccttaacaa ttccacctaa atctacaata atctttaatt catcataaat
     8881 ttctttagaa ttcctgtgat tcttacattt tatttgcttt gtaaaatatg gttgattata
     8941 tattatatat ccttgaggtg tagctattat ttcaaatcct ctagctctta taagggctat
     9001 atcttgtact attacttgcc tacttacatt taatatctca gctagcattc caccttttac
     9061 agggcactta gattctttta aaatatctat taatttgctt cttctttcat cagaattcat
     9121 attacacctt ctttctaaat caaaaacttt acctatacta tataattcta tattttatct
     9181 tattataaat caatattgat tttatattct atttaatatt aaactttatt gcaaataaaa
     9241 aagattacgt ggctacgtcc tactctccca ggcagtttcc caccaagtac catcagcgct
     9301 agagagctta acttctgtgt tcggaatggg aacaggtgta tcctctctgc tattgtaacc
     9361 acataatctt
//
LOCUS       DAGQWR010000152         9111 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.153, whole genome shotgun sequence.
ACCESSION   DAGQWR010000152 DAGQWR010000000
VERSION     DAGQWR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.153"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            117..278
                     /locus_tag="MIW95_003266"
     CDS             117..278
                     /locus_tag="MIW95_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152567.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            471..1100
                     /locus_tag="MIW95_003267"
     CDS             471..1100
                     /locus_tag="MIW95_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBY3152568.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            1339..2598
                     /locus_tag="MIW95_003268"
     CDS             1339..2598
                     /locus_tag="MIW95_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBY3152569.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLTYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            2857..3852
                     /locus_tag="MIW95_003269"
     CDS             2857..3852
                     /locus_tag="MIW95_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBY3152570.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRRNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            4098..5165
                     /locus_tag="MIW95_003270"
     CDS             4098..5165
                     /locus_tag="MIW95_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBY3152571.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHTGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSEDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            5702..7612
                     /locus_tag="MIW95_003271"
     CDS             5702..7612
                     /locus_tag="MIW95_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBY3152572.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILSYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(7675..8298)
                     /gene="ytaF"
                     /locus_tag="MIW95_003272"
     CDS             complement(7675..8298)
                     /gene="ytaF"
                     /locus_tag="MIW95_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBY3152573.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLELNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            8483..>9111
                     /locus_tag="MIW95_003273"
     CDS             8483..>9111
                     /locus_tag="MIW95_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3152574.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQRPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLL"
BASE COUNT         3850 a          884 c         1456 g         2921 t
ORIGIN      
        1 gtttgcagaa cgggtgtttt ttgaatttat tttaataatt tatgaatata tatcagaatg
       61 cataaatata tttaaatata aagattaaaa agaatttaaa ggagtgttga taacttatga
      121 taattccatc aagaagaata caagatataa cattaacaaa tttaaaaaat ggtgaagtaa
      181 ctttaattga attggatgaa atatacaaga agatgggatt tttatttgtt gttagtgaag
      241 gcaaattgaa aaagataaaa aaggagaatc atcattagaa atgaatgaaa taataaatat
      301 tataatagtt tataaaacct aacttatgta gaatataaat acttaagtta ggtttttttg
      361 tgataaagtt tataagtact caattttata agataaataa tggtcttaag tgaaaagtaa
      421 ataagtttaa attttagtaa gtattatatt gcataagagg tgatattatt atgaaaatag
      481 taacctacaa tattcataaa ggtatggatt caaataatag attaactcta actaaaatgg
      541 ggttatattt aaaacaattg gattgtgatg taatttgttt gcaagaagta ttatatcctc
      601 aatttttagc tttaaaagct gttttaaata tggatggtgt ctttgctaca aatgtaaaaa
      661 aagtaaatat gatatatggt atttgtacat ttacaacatc taaaatgtta aacagtaatc
      721 attttttctt aactagcaaa aaagaacaac ggggagctct ttgtataact atagatgcat
      781 atggaagaat tataaatgta ataaatactc acttgggact tgataggcag gagagagcaa
      841 aacaattaga cgaaataata gactatagga acagattagt ggggatagtt attttatgtg
      901 gtgattttaa tgagaaaaat gtctttttaa gtatgtttaa tgatatggct atttcactaa
      961 acaagtctta cttaccaaca tttgaaaaat caaactctag aatagattac atttttgtta
     1021 ataaaaatac tgaattaaaa ggatacacag tagaaaaaat ttacctgtca gaccattatc
     1081 ctgtaatagg ttacatttaa atttcaatat aaatgaaatt agttgttata aaataaacaa
     1141 ttttttgtta agaaatagta aaagaaatat aataaaaaat atcaaaaata aaactaatta
     1201 taagacataa atttacaata aagttacatt tgattaaatt ccttgtttaa caaaagctat
     1261 tcaaatataa tctaaggtag ttttgaaatg ggaactctat tatgcaaatt taagctagtg
     1321 tcaaaatgag gtgaagttat gggcagaaga acaacaagag tttcaagatt aggtaaagaa
     1381 aaaaaacgaa gtgttttaat tgtattggca gctttagttc taataatggg tggatttact
     1441 tattttttta acagtaaatt tctgtacaat gggaaaatcg ctaaaaatgt atatatagaa
     1501 ggagtaaatg tatctgatat gactaaagca gaggcattaa aagctataac agataagtat
     1561 actccagaag acttaaatct tacttatgat gggaaaaaat atacaatatc accaaaagat
     1621 attgatttaa aatatgatac agaagatgtt gtaaaagatg cttatgaaag tacaaaaaaa
     1681 gggtcctatt ttcaaaactt aaaaaaatat attgatataa gagtgaataa ggcaaatatg
     1741 aagataaaag ctgaatatga tgaggcaaaa cttagttcca aagtgtcttc aatagcagat
     1801 agtataaatg ttaagatgaa aaatgcttct attagtgttg gaagtggtgg actaagttat
     1861 acagactctg ttgtaggtag agaatttgat ttggcagcaa ataaagagtc tatatataat
     1921 atgataaaaa ataaagagca taaaacttta gaacttaaag taaatcttca aaaaccagat
     1981 ataacaactg aacaagtaaa aactgtaaat tctgttatag gtcaatattc aactacttat
     2041 tcacaagctg tagagggtag gtcatataat gtaggattat cagcaagaaa aactagtgat
     2101 gtattattaa tgccaggtga agaattttca tacaataaac ttacaggacc tagtaataaa
     2161 gcaaatggtt acaaagatgc acctgttata gtttatggta agctagaaca atcagcagga
     2221 ggaggtgttt gtcaaacatc ttcaacagtt tataatgcgg ctcttctttc gggtatggag
     2281 ataactcaag ttacaaatca ttcatctgca tctacttatg tgcctaaagg aagagatgct
     2341 actgtgagcg atggaggatt aaacttaaag tttaaaaatc catataagca tcctgtatat
     2401 attaaaaatt atgcaggtgg aggaagtgta tcatcagtaa tctatggtaa ttctggtgac
     2461 aaaccaaata tatctataga ggtaaaacaa actggtcaga ataaatattc tacatatcga
     2521 atatttaaag attcaagtgg aaaggtcata aaaaaagaac atatatcaaa tagttcttat
     2581 aaagaattaa aaaaatagat aattttttag agaaatattt gtaatataaa agggagtgtc
     2641 tcaaaatgaa ctttttagtt tatgagacac tctttttatg tgaaatcaaa tatattttca
     2701 tcatttcaac aatacaaaag aagcgtatct ctattttgag acagcctttt ttgtaaaaat
     2761 taacactatt agttcttgtt ttaatattac aatcattaat atatagaaaa tattcctata
     2821 tattggtata attgtaaata gatttagtta tataaaatgt atgaggaggt gacatggttg
     2881 aattttatag gcaatagata tgaggtagta aattctgatg acgttttaga aattaacaaa
     2941 atctataaag ctagagatgt tttttacagg agaaatgtgt taataaaagt tataaagcac
     3001 aataattata tttgtgaaga ttttgtttca aatttaattg atgaaagtac tgctttagat
     3061 gagataaact ctccttatat actaaaaata atagatgttg ggattcactg tacagaagaa
     3121 acaacattat attatgttgt aagtgaagac tttaatggta tcggactaga tgaattaatt
     3181 ctaggtaatt atcttcattt agaagcaatt gtaaacataa tgatacaagt tttaaaagct
     3241 ttagaaatga tacataggaa tcattcatat catggtagtt taaaatcgag tagtataata
     3301 gtagatacag aatacaatat aaaaatttgt gattttggaa ttacaaaagc aaataatggg
     3361 gttaatacaa gaagctatgg aaacaggaga tatttatgcc cacatcaact atgtataaat
     3421 tatactgata aagaaagtga tttttttgca acaggtctta ttctatttga atctatattt
     3481 aaaaaattgc cttttggaga atcaaattca gaagaaaaaa tgctacaatc tattgataag
     3541 ggattagatt ggaatagtat aaaagcaatt aatggaaata cagaacttat aaatgttata
     3601 aaaaggcttc ttggaagaaa taataagtat actagagcca atgatattat tattgattta
     3661 agtaaagtaa tgtatgaaaa agcatatata gaagttgaag atgagaaaga agaagaacag
     3721 aaacaagaca aagttataca agttaaaaaa gaatataaaa gtaaaatgag aaaaaaatta
     3781 cataaaaaac ttgttgttgc cggtctagct atagtcatga tttcaatgat tattattagt
     3841 tccataatat aaaaattgta tattaagtat tttaaaatca atgtctataa atgatataca
     3901 tagaaatttt agtgtagtct taaattatga aaggaagtgg ttattatgtt aacaaaaaat
     3961 aatgaggtat taaacgtcta ggagtatttt aacaaatagc cacctgcttt tacagtttag
     4021 aataaaaaag ctgtggctaa aggtcacagc tttttgctac tttttaaagg caggagaata
     4081 ttaaaaagga gcaaattatg agcaatataa aaataatagc aagtaataaa tgttggattg
     4141 aagatatagc aaggaaacaa ctagaagata tttcaaagtt aaatggaatt ttaagagtag
     4201 taggattacc tgacttacat acaggtaaaa tacctgtagg tttagcagtt gagactaaaa
     4261 atattattta tccacatata attggaaatg atataggatg cggaatgact ttatttaaaa
     4321 ctggggttct aaaaaagaaa tttaaaaaag acaaatggat taagtcactt agtaagatta
     4381 aagatttgtc cgatatagaa ataaaaaaca catataaaga agaatgtcca attttaaatt
     4441 taggaactat agggagtgga aatcattttg tagaaattca atgtataagt gaaatttata
     4501 ataaagaaca atttgaacaa ttaaaatttt catctgatga tattatgatg ttagttcact
     4561 gtgggtcaag aaattatgga gaagatattt taaaaaaatt ttatgataaa gatggacttg
     4621 aagttgaaag tgagaaagca atagattata ttaaaaaaca tgataatgct ttaatgtggg
     4681 cagagcgtaa tcgtgaagct atcagtaaaa aaattatgca gtcaataggt acatctgaag
     4741 atgttgaaac aatattctct ataaatcata actttataga gaaaagggaa gataaattta
     4801 tacatagaaa aggtgctgtt tcaagtgaaa gaggagctgt gattatacca ggttcaagag
     4861 gcagtctatc atatatagtt atgcctactg aaaatacaga gatatcttta tattctctat
     4921 cacatggagc aggtagaaaa tggtctcgtt cagtttgtaa atcgcgcctt aaaagtaagt
     4981 atagtaaaga tactataaaa caaactaaat ttaaaagtca gattatatgt aatgatgtaa
     5041 atttattatt tcaagaagct ccagaggcat ataagaatat agagcagatt attgaaagtt
     5101 tgatagaata tgaacttatt caagtagtgg caactatgaa gccattgatt acttataaag
     5161 gataattttt aaagttaaaa aagattaaaa ttatattata aatagctaat tttatggaac
     5221 atttaaaagt gttttattat tattagctat ttttttattt tatagagacg ataatttata
     5281 gagctaaatt ctttgatata taatgattac aaagttagat tgtattggaa actacacata
     5341 catgaattga tgacaatata agtaattcca aaatataaat gaaagtataa gcttattttc
     5401 actataaaaa ttttcactgt acaaaaacat taataacatt atttttattg acataaatat
     5461 ataatttatg gaataatatt agaagcactg gtataagtgt ttaaataaga atagaaaagg
     5521 agaagtgttt tatgaaaaaa aatactaaca ttaatgagtc cggagttaaa cttcttaata
     5581 aataaggagt ttgatatata ataactcctt atttattaag ttgttcgcaa aataactatt
     5641 atcaaagtaa atgatagggt gatttaaaag acgatgactt ataaataaag gagaaaaaat
     5701 aatgaatagt aatacatttg aaagattgca actaaatgaa gttaaagact taataaaaat
     5761 acattgtgta agttcgctag gaaaaaattt aatagataaa ttaactccta gtgggaatat
     5821 tggagtagta agaaggaaat tagcagaaaa taaagaagct agaaagataa ttgaaaatag
     5881 taatcacata ccacttgaag gattgtttaa tgcaggttct acaatagata aaattgaaaa
     5941 aggaatgata atagaacctg ttgaacttgt aaatattgaa gattttttaa gaggttgtag
     6001 gaagatgaaa gcttttatgt tagaaaaaga attttattca cctacattaa gttcttatgc
     6061 tttaaatata acagaatgta aaagtataga agatgagata aattactgta taaaatctaa
     6121 taaagttgat tctaatgcta gtaaagaatt aaaaaagata agacgaaata tagaaattac
     6181 agaaggaaag ataaaagata gattaaataa atttataaca tcaactgtaa ataaaaagta
     6241 tatacaagag tttataataa gtaaaagaaa tgatagatat gtaataccaa ttaaatcttc
     6301 ttataaaaat gaagttaatg gtactatatt agatacatca tcaaaaggaa atactgtatt
     6361 tattgaacct attagtgttt caaatttaag tacagagtta actatgctaa aagctgatga
     6421 aactattgaa gaatacaaaa tattatctta tttaacagaa cttatatttg agaagataag
     6481 tcagataaaa ttaaatatag agatactttc agaatacgat atggtatttg caaaagctaa
     6541 gtatagtcaa aaaataaaag gaattactcc aaaaataaat aataatggct atataaaaat
     6601 aattaaaggt aaacatccac ttctaacagg agatgcagta cctttagatt ttgaaatagg
     6661 caaaaattat agaagtctta taataacagg tcctaatgca ggaggaaaaa ctgtaaccct
     6721 taagactgtt ggtttactaa ctttaatggt tcaatgtgga tttgatattt ctgcaaaaga
     6781 aggtagtgaa tttagtgtat ttgaaaaagt ttttgtagat ataggggata atcaaagtat
     6841 tgaaaatgcc ttaagtactt tttcatcaca tataaaaaat attgcagaga ttatgagttt
     6901 atcaaataat tctactcttg ttctgtttga tgaaatagga agtggaactg aacctaatga
     6961 aggagctggt ttagcaatat cattattaga agagttttac aaaatgggat gtataactat
     7021 agcatcaact cattatggag agataaaaaa gtttgctaca ttacatccag aatttgaaaa
     7081 tgctggtatg atgtttgata aagaaacgtt agaaccattg tataagttaa ctattggaaa
     7141 atctgaagat agtaatgctc tttttatttc taaaaaaatg ggaataaaaa atagagtttt
     7201 agatagagct aaagaatatg taattgatag aaactacaat ttagatttag ttaaaagaag
     7261 taagctacaa tctaatattg aagataaaaa tagttctgaa gtcattttaa gctatacaga
     7321 ttataatgta ggggataagg taaaattact agatgaagat gattttggaa ttgtatataa
     7381 gcctatggac aagtttaata atgtagaagt attgtttaat gataatttta tttcagtaaa
     7441 tataaaaaga ttacaactta gcataaaagc atcagaattg tatccagaag ggtatgattt
     7501 agatgtatta tttactagtt ttaaagaaag aaaattagaa aaagatattc aaagaggttc
     7561 aaaaaaagct cttaaaaaga ttcagaaaga aattagagag aatagaaaat aaatataaaa
     7621 agcacaaaag aaagcaatct aaagagaaaa gtttatcttt agattgcttt cttattaaca
     7681 tatcttcata aatccaatga ctaacaagat taaaccactt aaccaagata agtttaaatt
     7741 tacgttttta acaaacatat ttcctagtaa acaaccaagt aatactgcaa ttatatttga
     7801 tattaaagaa aatagaataa cttgtatata attcacacct acaaggccac taccaaaacc
     7861 gatagctaat ccatctaaac ttaaagcaag tgcaagtgta aatgcttcag cagatttaag
     7921 cactttagat ttacttgtat ctaagacatc aggttcgata cttacattaa aatttaattc
     7981 aaggtctaac atattaaaat tataattctt tgagtgtata gattttttct taaaataaga
     8041 tttaaaaaaa ctttcaatta gtctagcact tcctaataaa aataaaataa agaaacatat
     8101 taacattgtt atattttgag gtaaaaaatc tctaactatt gtacctgcaa aaagcgatat
     8161 tgctaaaata gctgaacaaa ctacgctgat aattatattt gaatataagg gaactttaac
     8221 tttatttact ccatatccaa agcttgtcac aaaggcatct atacaaagag aagtaactaa
     8281 taaaattgat tcaatcacag tagattaaca tcctttctct taaattttgt acttatatta
     8341 gttatagtat tgtaaaaatg gataaattgt tacagataat ccaataaatt gttgataaaa
     8401 tttcaacatc atacaacacc taaataacaa aagaatcata tcatataata taaaaccaat
     8461 cataataaag caggtgaaaa taatggaaca acatagtaag gttgggaatt tcggtgattt
     8521 gttgaaagtt ttattagatg aaaaagaatt atcaatggga gaactaagta aaaaaagtgg
     8581 aattgataaa tctactatat ctcgtatagc taataataaa caaaggccaa atataaatca
     8641 tttagaaaag atggcaatac atttaaatat taacctagaa gaactgttga aagcttcagg
     8701 atatgaattt agaaacagca ataatcaaat atttaatgca gatagtgatt ttagtaactt
     8761 tgatgatatt cttggatttg caaatttaat caatgataag aactttaatg gaaatattga
     8821 aaaagagtta agtaagtgta agttatatgt ccaaacagat gaaggtaaaa aattactttt
     8881 tgataatttt aataaaaaaa tagattctat tgaaaaacaa ggtcaattta cagatagatt
     8941 aagacaaatg tatgctgatt tttgtacaga tggacttaca ataaagaaat atttattgat
     9001 tggaagtatg ctactttact ttgttatatc aacagatgtg attccagact tcgtgttccc
     9061 aataggattt atggatgatt tagtagcatt aaatatagtt acaaaattac t
//
LOCUS       DAGQWR010000153         9083 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.154, whole genome shotgun sequence.
ACCESSION   DAGQWR010000153 DAGQWR010000000
VERSION     DAGQWR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.154"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            268..924
                     /locus_tag="MIW95_003274"
     CDS             268..924
                     /locus_tag="MIW95_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152575.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1394..2665)
                     /gene="sleC"
                     /locus_tag="MIW95_003275"
     CDS             complement(1394..2665)
                     /gene="sleC"
                     /locus_tag="MIW95_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBY3152576.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIVAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGADTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            2910..3626
                     /locus_tag="MIW95_003276"
     CDS             2910..3626
                     /locus_tag="MIW95_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBY3152577.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            3705..5015
                     /locus_tag="MIW95_003277"
     CDS             3705..5015
                     /locus_tag="MIW95_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBY3152578.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            5115..5651
                     /gene="lepB"
                     /locus_tag="MIW95_003278"
     CDS             5115..5651
                     /gene="lepB"
                     /locus_tag="MIW95_003278"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBY3152579.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            5817..6353
                     /gene="lepB"
                     /locus_tag="MIW95_003279"
     CDS             5817..6353
                     /gene="lepB"
                     /locus_tag="MIW95_003279"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBY3152580.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6363..7118
                     /locus_tag="MIW95_003280"
     CDS             6363..7118
                     /locus_tag="MIW95_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBY3152581.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDIRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVREG"
     gene            7192..8859
                     /locus_tag="MIW95_003281"
     CDS             7192..8859
                     /locus_tag="MIW95_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBY3152582.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVILIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
BASE COUNT         3399 a          943 c         1695 g         3046 t
ORIGIN      
        1 taaatatatg gatgatggta gataagaata tcagaaaaaa atagataatt aaagggttag
       61 ggtgaaagag taatcgtaaa ataaatatat agataatggt ggatagagta gtaagaaaat
      121 aaatagataa agggatagaa taaatttaaa tatggaaaat gaatatctaa taataagtga
      181 ttaataaata attgtagata gattgtaaat taaataaatc tttaatagaa aggagaacta
      241 tttatatatt aaatagtatt tataaatatg aaaaaaggcg attttatatg gagtggagtt
      301 cttatttgta ttattgctgt cttagcaata ccaagtagta gagaagtatt tatggaagca
      361 acaggtgcac atccatatat tgggggtttt ttaaagtttg ctgtattagc tacaatggga
      421 gatttacttg gtgctagaat tttgtataag gattggaaaa taccagtagg agtattttat
      481 agagcaatag tttggggaat aataggtgta gttaccacat atggaatgtc aatctactca
      541 tcaggtgtag gtcaagcaca agtggatggt atgcttccat ttgagggaat agcttttgca
      601 aatgctctat ttaagagtgc aactatgaat atactaatgt caccaactgt atttttattc
      661 cataaggtta tggactgttt tatagataaa aaatatgagg ttggaaaggg gattaaggta
      721 aaacttaaag atgtggtaaa tattgtggac tggaatgctt accttggttt tagtgtttta
      781 aaaactatac catttttctg gataccatgt catacaattg tattcttatt ccctgctgag
      841 tatagagtaa ttgcttctgc atttgcatct atagctttag gtctaatatt ggctttagct
      901 aataagtcta aagttgcagt ttaggttcac tgatgaagat aatagtatta tttaaattat
      961 gaataacttt ttaaattaat attatttata tttataataa ataataattt agataagaag
     1021 gaatattcta agtgatagat agtgttttaa ataataagga gtaatattct aagtgagaag
     1081 tagcatttta aataataagt gagattttaa ataaaaagta agcttttcgt tagaatagca
     1141 ttttattaaa cttaaaaata aatataatat tttaaagaaa tctaagttgc taagcactgt
     1201 ttttaagaag cttaaaatcc cccatttaaa gttttaagat tctaaaaaaa caagttataa
     1261 ctaaaaaatg gctgttaaga ctacaatact tataagtaaa aatacttatg gtatatatta
     1321 gttcataaca gccattttta ttttaaatta ataattttat tcaataattt gagtttatgt
     1381 tctaaatcaa gctttaaatt aaaggattta aagaagctat tctagttgta gctacataaa
     1441 ctctggatat ttcataccat gttttaaaat ctactactcc gctttgaggt aaaccaaaga
     1501 ttccttggaa tatcttaaca gcgttttctg tatctgctcc atagatacca tcttctataa
     1561 cttttggtac agctggataa gaattagata tagcatttaa ctgattttgt atggttctaa
     1621 catactgccc agaagaacca acttgtagtg tagtaccagg gaatgacact ggtacaccac
     1681 ttactatagg tgctctttca aatactatct catctccata gaaatatctt aggatactct
     1741 catagtcata tccttggtca cctaagtctt tactgcccca ttgagtcatt tggtcaggac
     1801 attgagattt ttgtccatca caatattgtg ctaatagtgg ctgtcttgat gtaggaggtc
     1861 tttttataaa agtattaaaa atagcatcta caactacgtt aattggttca aataagtttc
     1921 tattatttat aaacttatgg tcgtatgctg ttgttgaagt tattgtaaag ttatatcctt
     1981 tatttctata ccattcagta aagactctat ttaaagtaaa tgatattata gctattacat
     2041 ttgcatagat tgtctgttca ggccaagttg aatatatttc agatgacgct atgtttttaa
     2101 tgtactcttt aaatggaatc caatagtttg gagctgatga atcttctggc aaaccatcat
     2161 gaactactat aaattcaggc actacagggt tatctaatac aacgaaacca gtaggaggtg
     2221 gtaatggttt taagttactt tctggaatct taggaggata agtaccatat agagtatgtt
     2281 ctcctatgtc aaaaatcaac tcactttgtc ttgagaaaga acgcttactt cttgttctag
     2341 gagacatggg aactccttgt cttgctacaa ttgtagctaa caactgcgtt ccttctatga
     2401 caactgtctc atatccatct gctatggctt ccactatata ctggctataa ggtctaacat
     2461 cagatggctg tagagagtaa tctacatctg gtgctggtaa tactaaacca gtcacttgac
     2521 cagattcatt acttcttagg ttttggtata aagttgatga gctttggcta ccattagaca
     2581 ttgaatatat gtttactact gcattttgaa taggtctatt gttagtagca tcaattatgc
     2641 ttactgttaa gaaaccatct tgcatcaaat caccctttct ttaaatgaat ttagttccct
     2701 tatatttcaa gatatgaata tagcttgaaa tttgttaaat aatatagata taaatttaaa
     2761 tttttttgag tatttgagta tatttgtatt taaataattt aaaataattt aatgtatatt
     2821 ttagtatgta tttaaaacac aaataagcta aaaataagta gatgatataa atcataaata
     2881 aatcaaaaat tttgaaagga aaataatcta tgaagctaga tttattttta ttagcaatag
     2941 ttcctatatt gatagggatg ttttggataa ggtctaaaga tagatattgt agagaacccc
     3001 taatccatct aattaaattt tttttaatag gtgcttttct aagtgttata attatacttt
     3061 tagaaaattt actgatgaag tttaatgtat ttgaagggta ttcagaacta atatatgtat
     3121 catttgtagt tgcaggactt gtggaagaag gtgtaaaagc tctgatactg attcctgccc
     3181 taattaaaga aaaacacttt actgaaaagt tagatggtat tatttactct gtatttttag
     3241 ctttaggatt tgcaacaatt gaaaatatgg tttatatttt ttctgaaagt agaaatctag
     3301 cactgcaagt tggtattaat agggcagtta tctctatacc agctcatgta atgtttgcaa
     3361 ttactatggg ctactatatt tctaagtata aatttgaggg aaataaaaat aaaagacgtg
     3421 agtatttgtt tatggcagtg ttaattccaa ttttattaca tggagttttt gatttcatat
     3481 tgatgataga atatagatgg gctattatat tacttatagt ttatgtaata attttgtgga
     3541 agataaatct tgataaactt gagaaatata tgaatcactc taaaaaagtg tttttcggta
     3601 atcttaggaa aaagaagaag aaataaaatt taaaggaaag acataaataa ttaaagtata
     3661 ataaatgaca cacaagtaaa gcagattgaa gggggattat agatttgaca gaattaccaa
     3721 aggaattttt agaagatatt gaagaaatac taaaagatga atatgatgat tttataaaaa
     3781 gttatgagga aagtaagact actgggctta gaataaatac tttaaaaatt gacaagaaaa
     3841 aattatttga tttggagttg tttgatttga atcaaatacc atgggcagaa gaaggttttt
     3901 attatgatga taaaattgac agacctggta gaaatccact gcatgaggct ggagcatact
     3961 atttgcaaga gccttctgca atgagtgtaa taccaaaggc agatattaaa gagggtgaaa
     4021 aagttctcga tatgtgtgct gctcctggtg gaaaatctac atatatacta tctaagctaa
     4081 acgatacagg tttattggtt tctaatgaaa ttaatgctag tagaattaag gctctagggg
     4141 aaaatttaga gcgatttgga gcgagaaatt gtattataac aaacaccgat tctaatagct
     4201 taagaaagac gtttaatggg tattttgata aggttattat agatgcacct tgttctgggc
     4261 aaggtatgtt taggaaggat gaggttgcca tatcagattg gagttatgca aaggtagtag
     4321 aatgtcaatc catccaaaaa gagattatca gagatggata taaaatgtta aagaaggatg
     4381 gcattttaat ttactcaact tgtacttttt caagggagga aaatgagcat gttataaatg
     4441 agtttatcag tgaatatcca catgcagaac ttattgaaat ggaaagatta tggcctcata
     4501 agataaaggg agaaggtcat tttgttgcca aaataaaaaa attagaagaa gaagaatgta
     4561 atgtcaaaga attaaaaata aaaaatttag ataaagaaat aaaggattat agggagtttg
     4621 aaaagaaatt tttaaaggtt gatttttata gtaataaaaa aaataaattt tatttaagag
     4681 gagaaaattt gtacctactt cctgaaatat atccagatac gaaaaaacta aaagtattga
     4741 gatacggttt gcatttaggg gttttaaaga agaatagatt tgagccttct catgcactat
     4801 ctcattactt aaaagttgaa gatgtaaaaa atgttgaaaa cttcagtgta cagagtgaat
     4861 caattcttaa atatttaaaa ggggatgttg tgaattctaa tgatagtaga ggatgggttt
     4921 tggtttcggt tgaaggaata cctttaggtt ggggaaaaga atcttctggt gtaattaaga
     4981 atcattatcc taaaggtctt agaaaagtat tttaaactat tagaaaaaag aagtataata
     5041 tttatattgt aggtttatgg tagattattt tgctgtagat tgacttaata tttaacacag
     5101 aggagacgat tttagtgggt gaagcagtta aaaaagaagt tgtagaatgg ataaaagtga
     5161 ttgtcatagc tcttgttttg gcatttgcaa taactcgttt tatagtgcca acaatagtca
     5221 aaggagaatc aatgtatcct acattagttg aacgtgatta tttgatagtt aacagaattg
     5281 cgtacaaggt aggagagcca aaatacaaag atataatagt atttaaaacc gacttaacag
     5341 aggaaaatgg aaagaaaaaa gatttagtaa aaagagttat cggggttcct ggtgaccatg
     5401 taaaaataca agactccaag gtatatgtaa atgataagtt gttagatgag acttcctata
     5461 tacataataa tcgtactgat ggagatattg atatcgtagt tccagaagga aaattatttg
     5521 caatgggaga taatagagaa aaaagtttag atagtagata cgatgaggtt ggattggtcg
     5581 acgagcatac cattttagga aaggttctag tcagattgta tccattttct aagataggaa
     5641 ctattgacta aacatataaa atttggagca atcgctgatt agctgaaatc accagaccat
     5701 acacaatagt tgtctatata aaaaagacaa caccttattt agtagactta tctaaaagat
     5761 agtgtttaag agacattatc aataaagata atataactat aaaggagaat ttgtgaatga
     5821 atgaaactat taaagaagag attgtagagt ggataaaaat aattattact gcactttttt
     5881 ttgcatttat tataactcgt tttataaaac caacactagt aaatggagaa tcaatgtacc
     5941 cgacacttaa atcacatgat tatttggtag caaataggat gacatataag ttatcagaac
     6001 caaaatgtgg agatataatg atatttaaga ctgatttatt acaagagaat ggaaggaaaa
     6061 aagagcttgt aaaaagggtt ataggtgttc ctggtgacca tctaaaaatt aaggattcta
     6121 aggtttatat aaatggtaag ttattaaatg aagtttcata tatacatgat aattatactg
     6181 aaggcgatat tgatatggtg attcctaagg gaaaagtatt tgcgatggga gacaatagag
     6241 aagttagttt agacagtaga tataaagaag tgggattggt agatgaagaa aatattaaag
     6301 gaaaagttat tttaagagta tttcctttta cagatatagg tatttttgag taggtgattt
     6361 gtatggcagt tggtatagcg tccttattgt ttaatattga ggaggcttta aacatttgtg
     6421 agagtataaa gcaaataact catttagaga ttggaattga caatatatct gaatgtagcg
     6481 agttatgtaa atataaagag agaatttcta agttaggatt atctataggg atacacttgc
     6541 ctatggaatt aaatacttgc gaaaacatag agtatatacg aaattcatgg ataaatttta
     6601 ttgagaagat agaatttgaa ctaaaaggat ttgatataag gtattttaac cttcatttgg
     6661 gatatgttat gacaaataga gttagaaaga atagagacaa atatttagga aatagtgtgg
     6721 attttttaga taaattaaat acaaattcat atgtatgtat agagaatact tattcaaaag
     6781 gtggggattt ttcaaatatt ggaaatatat cttatgattt tgaatatata tttaagagga
     6841 ttaaaaattc taagatttgt ttttgttacg atactggaca ctgtttaatt gatgaggatg
     6901 cttatgtaaa gaacttaaag gataaaataa gactgataca cttaagtgat aatgatggta
     6961 tcaatgatac acatgttggt ataggaagag gaattttatc tgaagagggg attaaagagg
     7021 tcttaacatt agatgcagaa tatttagtat tagaaataaa ttacgaagac attgaagata
     7081 ctataagtaa attgaataat attgtaagag agggttaaat acctctcttt atcttgttga
     7141 tatgcctcat taactaaaat ttatattcat caaaaggaag gagatgttga agtgaaaaaa
     7201 ataaacttaa aaatagaagg aaaagataag gaatattcat tagaagaaaa ttcccctgga
     7261 atcagattgg gagatatagc gaaagaattt tgtgatgagc ataagggtta tataacactt
     7321 gcagttgtag acaataaact aaaagagcta aattgtagag ttaagaagga ttgtgagata
     7381 aattttttag acactactaa tgaagatgga gagagagttt actttagagt catgtcattt
     7441 atatttgtaa tggcttgtag agaaatcttt tgggatagca gagttacaat agaacattca
     7501 ctatcagatg ggctatattg tgaagttcat atagatagaa agcttaaaga agcagatgtt
     7561 gaaaatataa aaaacaagat gaaagagata gtaaacaacg attatgttat agaaaaaatt
     7621 gaagttacaa gaaatgaagc tatttctata tttgaaaata atgaaatgta tgaaaaggca
     7681 gagcttttga aatacaaaga gtatgatagt gctaaagtat ataagtgtag aaactatata
     7741 gaccattttt atggttatat gttgccatca actggttata taaaatcatt tgatattaag
     7801 ctttataatg gaggcgttat aattttaggg cctagtgaag aagataagac tcttcctatg
     7861 aagtttgtac ctcaacctaa gctatcaagt gtatattatg aagcagaaga atggtcaaga
     7921 cttatgggaa ttgacaaggt agtatctctt aacaaaataa tagaaaacaa tgaatatgga
     7981 gatataataa ggacattcga agcattgcat gaaaagaagt tatcccaaat agcggatatg
     8041 ataaaggatg gcaataagag agtaatttta atagcagctc catcttcatc tggaaaaaca
     8101 agttttgctc acagattatc aatacatctt agagtaaata atttaaatcc tatttcaatt
     8161 tctttagatg attattttat agatagaaaa catactcctt tagatgaata tggaaattat
     8221 gattttgaat caatttatgc aatagattta gagaagttta acttagactt aaagaaactt
     8281 ttagctggtg aagaaattga tgatataaga ttcaatttta aagaaggtaa gagagaatac
     8341 actggtaaaa aaataaagat agattcaaat cagccaatta tattagaagg tatacatggg
     8401 ctaaatccta ttttaacatc atctatacca gatgaagata aatttaagat atacataagt
     8461 gcccttactc aaataaattt ggatgaccat aatcgaatcc ctactgctga tttgagaatg
     8521 ataagaagaa ttgttagaga ctataatttt agaggatata gtgctgataa tactatttta
     8581 caatgggcat cagttagaag aggagaaaag aagaatatat tcccgtacca agaggaggca
     8641 gatgctatat ttaactctgc ttgtgtatat gaattagcag ttttaaaacg atttgcgaaa
     8701 cctttgttgg aagagataaa ggaagataat cctgcacata ttgaagctac tagattacta
     8761 aagtttttgc agtattttgt tgaattggac gatacttctg acataccagg tatttcaatt
     8821 cttagggaat ttattggagg aagtaaaata gttgattaat atgtgtatta tagattgttt
     8881 tatgaagatt aaatggcata gcaagtagtg tgattggttt atgttaagta tattaagtgt
     8941 aaagtggaga attaaattga tacagatttt aaagtttgag ttgatatata aaaaggcttg
     9001 ttttatactt ttgtatgaag caagcttttt attttaaatt ttactttttt gtttaatatt
     9061 ttgttagttt atgtgataat ttt
//
LOCUS       DAGQWR010000154         9074 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.155, whole genome shotgun sequence.
ACCESSION   DAGQWR010000154 DAGQWR010000000
VERSION     DAGQWR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.155"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            590..1498
                     /gene="trxB"
                     /locus_tag="MIW95_003282"
     CDS             590..1498
                     /gene="trxB"
                     /locus_tag="MIW95_003282"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBY3152583.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1588..3411)
                     /gene="typA"
                     /locus_tag="MIW95_003283"
     CDS             complement(1588..3411)
                     /gene="typA"
                     /locus_tag="MIW95_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBY3152584.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3623..3865
                     /locus_tag="MIW95_003284"
     CDS             3623..3865
                     /locus_tag="MIW95_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152585.1"
                     /translation="MLEVTKIIIKYFASESDTFIKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            4013..4204
                     /locus_tag="MIW95_003285"
     CDS             4013..4204
                     /locus_tag="MIW95_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBY3152586.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4495..6996
                     /locus_tag="MIW95_003286"
     CDS             4495..6996
                     /locus_tag="MIW95_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBY3152587.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNVLAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            7156..7824
                     /locus_tag="MIW95_003287"
     CDS             7156..7824
                     /locus_tag="MIW95_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3152588.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7808..>9074
                     /locus_tag="MIW95_003288"
     CDS             7808..>9074
                     /locus_tag="MIW95_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBY3152589.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEKSELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVE"
BASE COUNT         3569 a         1051 c         1552 g         2902 t
ORIGIN      
        1 ttaaatatct gtaatattac aattttatac tacactatat tacaaaaaaa gtttacaaaa
       61 ctttaatttg aaaaatgtaa ttatatggta tatcatataa atataagtaa atagtgctaa
      121 tatttaaact attaatcata attggttcaa caaaagtata tagttaataa tgtttaattt
      181 gaagaaattg ttagtgtgaa attacatata tattcaaatt aaattcataa atatttcaca
      241 taataatagt atactgtaaa caaaaggagg tattaaatat gggaatagta cttataacag
      301 cagcattagt tttggttatg tatggacttt caattctatc atatatatca gaaagcaata
      361 tattagaaga cactgtaaat gataaccaat aaggttttga attatataaa taagtgagaa
      421 ctgtaaataa aagttaattt ttatttgcag tttttttata tatatgtaaa ataaagttaa
      481 ttaataaaaa ataatgtttt gtttgtagta tatatggata ataatagtaa agtataagaa
      541 tgatgagaat aatatataag aaataataat ataaagagag gtaaacatta tggtagatat
      601 cattgtaatt ggtgctggac cagcaggttt aacttcagca atttatgcta tgagagcagg
      661 gttaagcgtt acagtatttg aaaaaaatat ttatggaggt caagttgcaa gtacctctga
      721 ggtagaaaat tatccagcag ttcaaaagat ttctggggta gaattttcta ataatatata
      781 caatcaagca gttgctcaag gtgttgatat tcagtttgat gaggttgaag agataaattt
      841 agagggaaaa gtaaaggttg taaaaacttc ttcaggtgaa cataaagcaa aggctgttat
      901 tcttgcaaat ggagttgaaa gacgtaaatt aggttgtgca ggggaacaag aatttacagg
      961 tagaggagta tcttactgtg caacatgtga tggagccttt tttaaagata aagaagttgc
     1021 tatagtaggt ggaggaaata ctgcactaga agatgcttta tttttagcta ataattgtac
     1081 aaaagtgtat ttaattcaca gaagagatag ttttagaggt gaagaagtgt tggagaaatc
     1141 tgtgaaagca agagagaata ttgagatact ttacagtcat ggtgtagaaa aaatagaagg
     1201 agaaaagact gtatcgaaaa tagaagtaaa aaacttaaag actgaagaaa aaagaactat
     1261 agatgttagt ggtattttta ttgctatagg tcttaaacct aataataaaa tgtttgaaaa
     1321 tgttcttgat ttggatgaag gtggatatat aatttcagat gagtcttgta caacatcagt
     1381 agaaggtgta tatgtagcag gagatagtag aactaagttt ttacgtcaaa taataacagc
     1441 agcatcagat ggagctattg cagccgttca agcagctaat tatataaatg ttgaataata
     1501 taaatttcaa ataaaaatag ccatataaac tgaaataatt tttcagtact atatggctat
     1561 tttgttaatt agaggtaaat gtacgtttta tttaccttgt ctagatttgt tatatcttaa
     1621 tctatcatgt tcatttaaga atctttttct aagtcttata gaatcaggtg taatttcaac
     1681 taattcatca tcttctataa actctagtgc ttcttcaaga gtaaatattc taggaggaga
     1741 taattttata gcatcatctg aaccagaagc acgtacgtta ctcattttct tgtttttaca
     1801 agggttgact accatatcat cttttctgga attcatacca attatcatac cttcgtaaac
     1861 ttctacacca gggtctacaa acattacagc tctatcactt aaggcattaa gagaataccc
     1921 catagtaaca ccaggtcctt gtgctattaa aacaccattt cctcttgaag gtatttctcc
     1981 tttaaattct tcaaattttt caaatgaacg aactaaagta ccttctccac gagtagcgtt
     2041 tataaattca cttcgatatc ctaaaagacc acgagtaggt gctaagaact ctatttttac
     2101 atagtctcct tctattgaca tagactccat catacctttt ctcatgttta attcatttat
     2161 aatagtacca gagtaaactt caggacagtt tacaacaact ctttctattg gttccattag
     2221 ttttccatct tctttatgca ttaaaacttc aggtttagaa acaccaactt cataaccttc
     2281 acgtctcata ttttcaagta gtatagataa gtgaagctct ccacgtccag aaactttgta
     2341 tccatcagta gtatcaagag gttcaacttt aagaccaaca tttacttcaa gttccttttc
     2401 aagtctatct ttcaaatgtc tagttgtaac gaattttcca ctttttccaa cgaatggaga
     2461 gtcgtttact aagaagttca tagaaagagt aggctcttct atttttatca tttccatagg
     2521 tagtggacta tctaaatcgc atatagtttc acctatagat atatcaggaa taccagctat
     2581 aacaactatt tctccactag tagcttcatc aacttcaact tgttttaaac cttcatacac
     2641 agaaagtttt gatacttttc ctttagatac aacagaatct tctctacata tagcaacttg
     2701 tgtattgttt ttaagagtac ctttgtaaat tctacctata ccaagtctac ctacataatc
     2761 atcatatgca agagctgata tttggaattg tagaggttca ttatcgtaat taggataagc
     2821 ttcaacgtga tttacaatag ttttaaatag aggagaaagg tcttcactat catcatccat
     2881 ttcaagttta gcaatacctt gtttagcaat accatatatt ataggaaatt cgcattgctc
     2941 atctgttgca tttaagtcta caaataaatc gaaaacttca ttaactactt cttccgctct
     3001 ctggtctttt ttatcaatct tgtttataaa taatattggt tttaaaccga actctaaaga
     3061 tttttgtaaa acgaatctag tttgaggcat tgggccttca ctagcatcaa ctaatagaat
     3121 aactgtgtca acagttttca taacacgttc aacttcagaa gaaaagtcac tgtgtcctgg
     3181 agtatcaact atgtttattt tgtaatcttc atagtttatt gcacagtttt ttgaatatat
     3241 agttatgcct ctttcttttt caaggtcgtt actgtccata acgcagtctt taactacttc
     3301 gttctttcta aaaacaccgc tttgagaaag gaaagcatca accaatgttg attttccagc
     3361 gtcaacgtgg gctataactg cgatatttat aattttatgt ttttgtgaca tttttgttgc
     3421 tcccttcgat taaaataact tattaattat agcacaatat attattaggt acaaggagga
     3481 atatattaaa ttgtattaat atagtttaaa ttggataaaa ctgttagttt tgcatctgaa
     3541 aattattctg tttaaaaaaa ttttttagac aaataataat cttaatagat aaaaagatta
     3601 ataaaagagt aaaggatgat atatgttgga agtaaccaaa ataataatta aatattttgc
     3661 aagtgaaagt gatactttta taaaagaaga agtttgtaat tttgaagaac ttcttaagtg
     3721 gtctcataaa tttattgaag gtaaaaagat agaagatgtt ttgtatataa agtctaaaga
     3781 aaactataaa gtaaatgtga ttaagtcttt agaaattaag gaattggaaa tagtgagaaa
     3841 acaacagata ccaccgtaca tgtagtatta ataatatata aaattaatat gtaatactca
     3901 caataggata taaatggtat atatggtaat aatttatatt aaaaaaactt ttataaagtt
     3961 tatatttggg tataaaagta ttaattagac attaattagg aggaacttaa atatgataag
     4021 agtatgtcca aactgttcaa atgtggatgt aaataagcta aaaactttgg ttggagaaga
     4081 aaatgtaaag actggttgta taggtcagtg tagagcattt aaaaaagaag ctgttggaat
     4141 aatagacaat gagcttgaaa taaaggaaac agaagcagag ttttttgaag catgtaaaga
     4201 ataaatttta aataaaatat aaattataat aaatagagca tctaaattgt ataagcatta
     4261 aacaatttag atgctctatt tttatatata catttaatta tgagtatact tttctattaa
     4321 atacaaataa atataactaa tacatttatt tattaaaaaa atattgattt aatataccta
     4381 tagggggtat aataaaaata tagatttcta aataagtaat ttatcaatat tacatatcaa
     4441 aataaaacgt tacaaattag ctgaaaatat attgaaagaa aggaagattt tattatgagt
     4501 tcaaataaag tggtagaaag ttataaaatt acaggtatga catgtgcagc ttgtgcaaag
     4561 gcagtagaaa gagtaactaa gaagatggat ggagtatatg accaaagtgt aaatatagct
     4621 actgaaaaac ttaaaataga atatgataat tctaaggtta attttgatga tataaagcag
     4681 gttgtagaaa aagcaggata tggaattata aaggaagaaa gtaataaaaa aattgatatg
     4741 aaaattgatg gaatgacatg tgcagcttgt gcaaaagctg tagagagagt ggtaaaaaaa
     4801 ttggatggag tagaaagtat tagtgtgaat attgcaacag ataaagctaa tatagactat
     4861 gacccatcaa aagtaaaatt atctcaaata aaagcagcta ttgaaaaagc tggatataaa
     4921 ccaatagaag aggtaagaaa taaggttgat gtggatgaag ataaattgag aaaagaaaga
     4981 gaaatgaagt ctttatttgt taaatttata gttgcaatag tatttgcagt acctctgttt
     5041 tatatagcaa tgggacctat gataataaag cctatagggc catggccttt gccagaaatt
     5101 ataaatccta tgacaaatac ttttaattat gcattaattc agttaatttt agtaatacca
     5161 gtaatgatag ctggttataa attttatata aatgggttta aatcactttt ttctttaagc
     5221 cctaatatgg attctttagt tgcaatagga acattagccg catttttata tagtctatat
     5281 acgacactac aaatagccaa tggacaaatt caaggtatgc accatcatca gttgtattat
     5341 gaaagtgcag gaataataat agcacttatc ttacttggta aatatttaga atcaaaatca
     5401 aaaggaaaaa catcagaagc aattaaaaag cttatggggc tacaacctaa aactgcaata
     5461 gtgttagttg atggtaaaga agttgaaact cctatagaag aagtagagat aggagatata
     5521 ctattagtaa aaccaggaac taaaataccc gtggatggag ttgtaataga aggatataca
     5581 tcagttgatg aatcaatgct tacaggtgaa agtataccag tagaaaaaaa tgttggaagt
     5641 aaggtgacag gagctagtat taataaaaat ggtgttatta agtttaaggc tgaaaaaatt
     5701 ggtggagata ctgctcttgc ccaaataata aagttagttg aagatgcaca aggtacaaaa
     5761 gcacctattg ctaaacttgc cgatactgta tctggatatt ttgtaccaat agtaattgca
     5821 atagctgtag ttgcatcatt attgtggttt ttaataggag gaaaagatat agtatttgta
     5881 cttactatat ttatatcagt acttgtaatt gcttgtcctt gtgcattagg attagcaaca
     5941 ccaactgcta taatggttgg gactggaaag ggcgcagaaa atggaatctt gataaaaggt
     6001 ggagaggcac tagaatctgc acataaagta aatacagtga tatttgataa gacaggtact
     6061 ataacagaag gaaagccaaa agtaacagat atagtattaa ataataatgt aaaagaagaa
     6121 tatcttatca agattgcttc aagtgcagaa aaaggttcag agcatcctct tggagaagca
     6181 atagttaagt atggagaaga gaagaatata aaatttgaaa aagttgataa ttttaaagcc
     6241 ataccaggtg ctggtataca agttactatt aatgatgaaa gtatattact tggaaataga
     6301 aagcttatga atgacaataa tataaagctt ggggatttag aagaaaaatc aaatgtattg
     6361 gcaagccaag gtaaaacacc tatgtatata gctgtagatg gaaatttatc aggtataata
     6421 gcagttgcag atgtagtcaa agaaagtagt aaaaaagcaa tagaaatact tcatgatatg
     6481 ggaataaaag ttgcaatggt aactggagat aatgcgaaaa ctgcaaatgc tatagcaaat
     6541 caagtaggta tagatatggt tttggcagag gttttaccag aagataagtc aaaagaagtt
     6601 gaaaaactac aaaatcaagg aaagtttgtt gcaatggtag gggatggaat aaatgatgcc
     6661 ccagctcttg caaaagcaga tatagggata gcaataggaa gtggaacgga tgtagccatt
     6721 gaatctgcgg atatagtact tatgaagagt gatttaattg atgttccaac agcaattaaa
     6781 cttagtcatg aaactataaa aaatataaaa caaaacctat tttgggcatt tggatataat
     6841 acgattggta tacctgtagc agcaggaata ttatatgtat ttggaggacc tttattaaac
     6901 ccaatgattg cagcagcagc aatgagctta agttcagttt cagtcgtatc aaatgcactt
     6961 agacttaaaa acttcaaggc atataagaga gattagatta ataaattaaa taaaatgtat
     7021 ccaaaagagc tatctttaaa ttaaaaggat taatttacaa attaagatag ctcttttttc
     7081 atgtaaaatt cacaacaata agataaaata taagtaatat acgaaatttt aaataaaata
     7141 gaaaggattg atagaatgaa ttcgtcaata cttgtaattg aagatgattc aaatatacaa
     7201 gagttaatat cagaattttt aagtgcagaa ggttatcaag ttgatacagc aaatgatggg
     7261 ttggaaggta tacaaaagtt taagcaagga agttatgatt tagtaatatt agatataatg
     7321 atgccaaact tagatggtta tggagtttgt aaaatgataa gaaagtcatc aagcgttcca
     7381 attatatttt taactgcttt aaatgatgaa ggagaccaac ttaaagggtt tgacttagaa
     7441 tgtgatgatt atataacaaa accattttca tttaatcttc ttataaaaag agtagaagca
     7501 attttaaggc gaagtaacaa gactataaat gacaaattta tagtttttga aaaattaaaa
     7561 ttagatttaa acacatatat tgcagagata gatggagaac ctatagaact tacactaaaa
     7621 gagtttaata tattaaaagc tttgatagaa aagtatccac aagttataac tagggaaggt
     7681 cttttggata gtatatgggg ttatgactat tatggagata ctaggattgt tgatgcacat
     7741 ataaaaaata taagaaaaaa aatatcgtta ccatatatta aaactgtaaa aggaatagga
     7801 tatacacttg agaaggatat ttgataagtg ggaaaagtta agtataaagt ataagttatt
     7861 ttcaataacg acatcattat taatagcgtt agctttaata atatatctaa ttttatattt
     7921 tttattgcca tcatattatc atgagtataa aattgagtct cttcaagaaa gtttaaaatc
     7981 tttagtagat agttcaattc atttcgatac atatacttta gaagaaagac tttattatat
     8041 ggctaaagac caaaatttgg ctattttatt aaaagataac caagggaaaa ttgtatatgg
     8101 taagaatgaa gtagttattt taagatatag taagtacatg attaatagtc ttgaggatga
     8161 gtatagaact tctataccta tatatactaa agatgcaaaa gatggtccat atactttaga
     8221 actcgttatg ccattacaac caattgacga agcaaacgaa gttataagaa aactaatgcc
     8281 atacatcata tcaatagcaa tattgatagc gattattgga gcatatatat attcaattgt
     8341 aataacaaag cctcttataa atataataga gagtgagaga gaacaggaat atagaaggaa
     8401 ggattttgtt gctacaatat cacatgaact aaaaactcca ataaccataa taagtggtca
     8461 aattgaaggt atgatttata gtgtaggtaa gtataaggat agggatactt atctgaaaaa
     8521 atcatatgaa tgtacacaag aattaaaaga tttagtaaat gaaatgatag aggtttcaaa
     8581 atcagaaata cttgaaaaag atttgaaatt ggtatcaata aatataagtg aattattaaa
     8641 tagacttgta aagagacaag tatttttaat agaagaaaaa catatgaaaa ctattttaaa
     8701 aatagaagaa aatttggaaa ttaaagcaga ccaagagaga ataactaagg caatcaataa
     8761 tataataaat aatgccataa aatattctcc tgaaaaatca gagcttataa ttagactcta
     8821 tgataaaaat aaacgaataa gtaaaaagac ttctaatcaa agagtggttt tagaaattga
     8881 aaatactggt gtaactatcg agaaacgtta tttagaagag atatttaatc cattttatag
     8941 aatagaaaaa tcccgttcaa gaaaaactgg aggaagtgga cttggacttt atatagtcag
     9001 tcaaattttt aaaagtcatg ggtttgatta tagcataaaa aacaaagaaa actctgtagt
     9061 attcacagta gaat
//
LOCUS       DAGQWR010000155         9063 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.156, whole genome shotgun sequence.
ACCESSION   DAGQWR010000155 DAGQWR010000000
VERSION     DAGQWR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9063
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.156"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            30..1034
                     /locus_tag="MIW95_003289"
     CDS             30..1034
                     /locus_tag="MIW95_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY3152590.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNAALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGIKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            1159..2271
                     /locus_tag="MIW95_003290"
     CDS             1159..2271
                     /locus_tag="MIW95_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY3152591.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSVPTSAEKDKLNSKSKNEEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            2694..3455
                     /locus_tag="MIW95_003291"
     CDS             2694..3455
                     /locus_tag="MIW95_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152592.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            3458..4276
                     /locus_tag="MIW95_003292"
     CDS             3458..4276
                     /locus_tag="MIW95_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBY3152593.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            4456..5058
                     /locus_tag="MIW95_003293"
     CDS             4456..5058
                     /locus_tag="MIW95_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBY3152594.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    5568..5806
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5870..7789
                     /gene="thrS"
                     /locus_tag="MIW95_003294"
     CDS             5870..7789
                     /gene="thrS"
                     /locus_tag="MIW95_003294"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBY3152595.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            8557..8985
                     /locus_tag="MIW95_003295"
                     /pseudo
     CDS             8557..8985
                     /locus_tag="MIW95_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3667 a          890 c         1635 g         2871 t
ORIGIN      
        1 tatcgtaaat gtatgaggag tgaaatttaa tgcgtttcaa aagaatattg atattgatgt
       61 taactgtagc aatgatagct ggaatgttgg ttgggtgtgc taataaccca agcaataatc
      121 ctagctctag caaagataat aaaagtgata taagcgataa aagtaattca aaaaaatctg
      181 tatcaatgat actagatatt gaaggaacaa ataatgaagc tatgaataat gcagctttat
      241 tagcattaaa caatgctcaa aagaaactta atatagatac aaacaaagtg gaatcagatg
      301 attcctcaac tttttctaat agtatagata ttttatgtaa tgacaattac gatttaatta
      361 tagctgtagg agctagattt gcaaaaccat tggagatggt agccaagaaa tatccaaagc
      421 aacaatttgc aattatagac tatgagtatg acaagcaacc ttctaatata acttcaattt
      481 cttatgaaga taataaatct ggatacctag ctggattaat tgctggaaaa atgactgaaa
      541 gcgataaggt tggttttata ggaggaataa agagttctag tagggataaa tttgaatcag
      601 gttttagaga gggtgttaaa ttttctaata gtagtataaa ggatatttca gttgagtatg
      661 cagatgtttt taaagatagc aaatcagtag aatctatagc taaaaaaatg atggataatg
      721 gagtggatat tattttttct actactgaag atgattcaaa agctgtaata gatgcagtaa
      781 gggcaaaaaa taaaaaagtt atagcaacga ataaagacca acatgagtta gctccagaaa
      841 atgttataag ttctattgta aaagattttg agaatcctac ttataatctg atacaaagtt
      901 ttgtaaaagg aaattataaa ggcggaaaag ttatagaaaa cacagttaag tctggaagta
      961 ctgggcttgc ccaaaatagt tctcaaaata ttcctccaga tgtgttagaa tatgttaata
     1021 agaacaataa ataatctact ataatatcca agaatactta tgactttagt tatgaggtga
     1081 attggatgtt ataatagaca tatatagaaa gacgtatgca gaggtaggca gacctatcta
     1141 aatcagggag tgaattttgt gaaaataaaa aggatattga tggcttgctt tgtagtagtt
     1201 tttacaattt ttatattagc tatatgtaac tttgattttg gatttgataa agtaaataag
     1261 aataattcta atatagatag tgctaaatcg aaacagagtg agtctaaaag tgtaccaact
     1321 tcagcagaaa aggataaatt aaatagtaaa agtaagaatg aagaagatga cacagaagat
     1381 gattcatatg taagaaaaac tattggagta ttgttagata ccgaaaactt aaataacgaa
     1441 tcatataata ataatgtact atcagcctta aaaagggctg atgaagattt tgatgttagg
     1501 ataaaacttg aaaaaccaaa gacttctaaa agttttaaag agagtataga aaaactacat
     1561 aagtatgaac cagatttgat aatagcagtt ggagctagat ttgcagagcc aattaaagaa
     1621 gctgctacta aatatcccaa acagcaattt gcaatagttg actatactta taaagttcag
     1681 ccagctaatg taatttctgt atcatttcaa gataatgtta ctggatattt agcagggctt
     1741 attgcaggta aaatgactga tactgggaaa gtcggtttta taggcggagt tgagggttct
     1801 agtagagata agtatgaatc tggatttaga aaggggctta aaacttcaaa taaagatgct
     1861 gagttactag ttaagtattc aaatatattt gaaaaccctg aatcagctag aactattgca
     1921 aaggaaatga gagattctga tagagatgtt gtattttcag ctacagaaga tgatagtaaa
     1981 agagctattc aggtagcaag agaaaatggt ggtaaagtta tagctattaa taaagaccaa
     2041 tacgatatgg ctccagataa tgtaataagt tcaattataa aagatgtaga acaacctgtc
     2101 tatgaactga taaagaacgt tgcaaaagat ggttttaagg gaagtaaagt aatggaattt
     2161 aaaataaaag atggagaact tggcttgact tcaaagagca gtagaaatat accaccagat
     2221 gttttggaat acataaaaaa agagtataac aaagtgaaag atacatatta attattgatg
     2281 tagaagatta taaaaatata ctgtttaagt ttagattagc aataaaaaga tatatttatt
     2341 aaatttgtgc taactgtaca aaatatattg attaagttta gattggtgat aaaaagatat
     2401 atttattaaa tttagtctaa ctgtacaaaa tatattgatt gagtttagat tagcaataaa
     2461 aagatatatt tattaaattt gtgctaactg tacggaatat actgcttaag tttagattag
     2521 tgataaaaag atatatttat taaatttgaa ccaactgtac aaagctatac taattaagct
     2581 tagattaact atataaaatt ataacaataa gtgtgtgggc agaaaaattc tgctacacac
     2641 tttatttata ttcatataaa aaaattatca aaaaaaagaa aaaggagtaa aaaatggaat
     2701 ttacaagaga accatttata ctagatgacg atttgtgtct agctttggtg gacaaaagaa
     2761 ttagttatct aatggaaaat gaattaaaat taagaaatat agagataata aaaacaatag
     2821 aatgtaaaga attatatgaa agcataaaat atcacccgga tatatgtatt tgtaatttag
     2881 gaaaaggaga tataattgtt gcgccgaatg tatataatca atacaaagag ttattaagta
     2941 gatataaatt taatataatt aagggagaag ctaatttatt aaatagatac ccttatgata
     3001 taccttacaa tattgcgata gttggtgact atgctataca caattttaag tatactgaca
     3061 aagcaatatt agattatatt gaaaataata aacttaaaaa gataaatata gagcagggct
     3121 attctaaatg ctcaatctgt atagtagata gaacgtctat aataacatca gataaaggta
     3181 tttggaaaag tatgaagaat acagaaatag agtgtttatt gatagaaaaa ggtcacataa
     3241 atttatttga gatgaattat ggatttatag gtggatgtac agggttgatt tctaaggata
     3301 aactagcttt ttgtggagat gttaaaaaac atcctgacta tgaacgtata aaaagttttg
     3361 tagaaagtaa aaacaaggag atagttacac ttagctgtga aaatttacta gatataggtt
     3421 ctatagttcc tctcatgaca agaaaggagc gataactttg gttgagaagg ataatatttc
     3481 ttccatgaaa tttttgagga ataataatgt aaacgataga ttaatatata cgaatgacat
     3541 tgtagaagta gtgatagatt ctgcaaagca agaagtgtta aataaaaaaa tacttaatgg
     3601 tactaaagct gaacaaagag ctttaaatat tgatgagata tcaacggaaa taactagtat
     3661 gataataagt ataatgaaag agaaactttt gaaagaatac attctaaaat gctatgggcg
     3721 tacatatcct gaagataaaa ataatatata catatattca ctgaacatat ttgctaaaaa
     3781 agagattttt atgagagaaa cagtatttac tagggtgaga aattatatag aagaaaatga
     3841 ttttataaat gtagaaggtt ttattagatt tagaatgaga gaatttatga aatatatttc
     3901 tgctataggt gatattgcag ttgaggagta tttaataaag aaagaccaag atgaatttat
     3961 aagagtttta aaatacttta tagatactca agaagaaaag attgatttgc ttaaagttca
     4021 tataatggaa gataatacat ttgttttata cgataaaaat ggaaataaga tagacagtat
     4081 agatgatgaa gaaattataa atatggtaat aagagaaaat ttaaattatg aggacttttt
     4141 aataagtacc ttattaactc tatgtcccaa aaaaatagaa atattggatt tgttaaataa
     4201 taattgctca aaggaaatta tagatactgt agaagcaatt tttgaaaata aagttagtat
     4261 aattatggaa aactagaatc taagcatata aaagctagcc atataaaatt aaaaaaattc
     4321 tttttaaatc gacacaaaag acttataatt gtatataaag ctatagtata actagagtta
     4381 ttaagtataa agaatatcta cttgattaat gcaatatagc tggcaaatat acaattgata
     4441 aggagtaatt ttataatgga taatttaatt aggatactga tattagctgt tattggtgga
     4501 tttataggtt atgttacgaa tgtagttgct ataagactga tatttagacc aatagagcct
     4561 attaaaatac ctattttaaa tatagaaata gtaggtctta taccaaagag aagagcagaa
     4621 atagcagcaa atgtaggaga aataattcaa gaagaatttc tttctatgga cgaaatactt
     4681 gcaaatatca ttactgacga agataaggaa gaagttgtaa gatacataaa agcaagggta
     4741 aaaattataa tacatgagaa agtttcattt atcccgagtg gtataaaaaa catgatacaa
     4801 gattatctag gagagataat tgagtctgaa gttaagcaaa gtatagatga attgagtaaa
     4861 aatataataa ataaagcaaa tgaacgtata gatatacaaa aaatggtcga agataagatt
     4921 aatgaactag atttatatga attggaagag ataataataa gaatagctaa gaaagaatta
     4981 aaacatatag agtttttggg gcttgtctta gggtttttaa taggtatagc tcaagggtta
     5041 attactatgt ttatttaaat atatgagaag tagcatttat ttaagtacta attatattta
     5101 cctatttgag tgaattactt attaatagta tttagatatt aattgtattt acctatttga
     5161 gtgaattact tattaatagc atttagatat taattgtatt tatttatttg attgtgttac
     5221 ttattaatag tatttaagta tttactgtac tacttattaa ctatattaag tattgattgt
     5281 atttaagtat acaaaaccta aaaagagtac aaaaattaga agttagtttt aataaaaatt
     5341 agttttagta tttatctagt catatctatc atgcatgtgg atacgtttta aaaattttaa
     5401 atttatgaaa atattataat atcactataa atcaagagat taatttaata taaaaatata
     5461 agaatattgg tttacagctg attgagatga aaaataatgg gtaattaata tattacatta
     5521 atgttgattg atgtataact atctgttata atgaaaatat attaaaaatc atagtgttga
     5581 agaggaagag tagagtagaa accattctca gagaatttgg gtctatggct gagagcccat
     5641 gtaataggtc tatttgaaaa ctaccttgga gctgagctta ttagcagtat ttgtatttgt
     5701 actatacgtg aaagtgctcg ggtgacgacc ttatagtcat caagagggtt gtatatactt
     5761 gtatacgcca acttgggtgg aaccgcgaat taatatcgtc ccaaacatat ttggggcgat
     5821 tttttattac aaaaaatcta aaaattattt caataaggag gaaaaaacaa tgattaaagt
     5881 tgctttaaaa gatggctcaa taaaagaatt tgaaaatgct atttctgtta tggatgtagc
     5941 taaatccatt agtgaaggat tagctagaaa tgtggtagca gcgtctgtga acggagaggt
     6001 tgtaggtctg gaccacatta tagatactga ttgtgacttg aatttattta agtttgaaga
     6061 taaagaaggt aaggaagttt ttagacatac atcagcacat atattagctc aagctataaa
     6121 aagactttac ccagaagcaa agcttgctat aggaccaagt atagaaaatg gattctacta
     6181 tgatatagat ttagaccata gattagttcc agaggaccta gagaaaatag aagctgaaat
     6241 gaaaaaaata gctaaagaag atttaaaaat agaaagattt gaacttccta gaaatgaagc
     6301 tttagagctt atgaaagaac aaggagaaga ctataaggta gaacttatat ctgatttacc
     6361 tgaaagtgaa ataatatctt tctacaagca aggtgatttt actgatttat gtagaggacc
     6421 acatttacca tctactaaaa aggtaaaagc tgttaaatta caaagtgtag caggtgcata
     6481 ttggcgtggt gacgaaaata ataagatgct tcaaagaata tatggaactt cttttgagaa
     6541 aaacaaagac ttagaagagt acttacattt actagaagaa gctaaaaaga gagaccacag
     6601 aaaattaggt aaagaattag gattatttat gatacctgaa gaaggtccag gattccctat
     6661 gtttttacca aaaggtatgg aacttaaaaa tgaattatta aaattctgga gagaaataca
     6721 cagaaaagct ggatatattg agatagaatc tccaatcata ttaaatagaa aattatggga
     6781 gacttcagga cactggtatc attacaaaga aaatatgtat acagttaaaa tagatgatga
     6841 ggattatgct ataaaaccta tgaactgtcc tggtggatta atttactata actctcaact
     6901 acattcatat agagatttcc caatgagagt tgcagagctt ggtagagttc atagacatga
     6961 gttatctggt gcattacaag gattaatgag agttagagca tttactcaag acgattcaca
     7021 tatattcatg ttaccagagc aaattaagga cgaaataaaa ggagtagcta acttaataga
     7081 tggtatctac aaaacttttg gatttgaata taatttagaa ttatctacaa gaccagagaa
     7141 ctctatggga agcgacgaag agtgggaagc agcagaaaat ggattaagag aagctttaga
     7201 agagttaggt ctaccatata ctattaatga gggtgatgga gcattctatg gtcctaagat
     7261 agacttccat ctaaaagatt gtttaggaag aacttggcaa tgtggtacta tccaattaga
     7321 tatgcaactt ccaagacaat ttgataatac ttatattggt caagatggtg aaaagcatag
     7381 accagttatg attcataggg ttgcttttgg tagtatagaa agatttatag gtatattaat
     7441 agagcattat gctggtaaat tcccagtttg gttatctcca actcaagtta agatacttcc
     7501 tatatctgat aaatttatgg attatgcaaa tgaagttaag aaagagttat ttgataaggg
     7561 tataagagtt gaattagatg atagagctga aaaaattgga tttaagataa gagaagctca
     7621 gcttgaaaaa gtgccataca tgttaatagt aggtgaaaaa gaagtagctg ataataatgt
     7681 ttctgttaga tcaagagata aaggtgaaat aggtagtatt aaattagatg aatttatagc
     7741 tagtatttca aaagaaattg aatcaagaga aagtattatt caagactaat atataatata
     7801 ttagtagcta agatattgtt atataagata agtttaatca atatgttgat atgttgatac
     7861 atagtgatta aatttatgaa aagatgagta taattagagg tacagttaat catattttta
     7921 tgattaatgt acctctagtt ttttgtatgg tagatttatt aataaatata tcatagaagt
     7981 gttttgaaca tggaaatatt tatgtgcatt ctttaaataa aagtttattt tttgaatgat
     8041 aattaagtgc ttgttggtaa ggttatggct gtaaaaatca taactttatt tttatatttg
     8101 ttccattttc ataaaaatta ttatcccttt aagaaaattt cttaatcctc ttgaaatatt
     8161 tcctttttag tctaaagtta aatttctttc ttcaaaatga atatttaaat gaactaaaat
     8221 ttaagtacat tttctaatct tatgagtcta tttttcatag ataattgccc tataatagaa
     8281 gaatctatgt tggggctatg tggtgacaat aacagcattg tcagtgatta attaatgctt
     8341 tataaatgat taattagtgt tttatgttat ttttggactt accctctata catagattaa
     8401 tttatttaac tttagatata gagagaatgt caagtacata cacagacatt agagtttaag
     8461 aaagtgttta tggagcaaga acttttaaaa gaagtagtga cttagtttaa tttcaagaaa
     8521 tgataaatac aaaagaggaa aaagagaggt gttgaaatga tagaaaaagt agaagaaaaa
     8581 tctaagatta ttaaaacatc aaaagaatat aagcgaacta taattgaaga aaaagataaa
     8641 gaatggacca aaaaattaaa tgatatactt agtagaagta gtttttgtaa tgatgaatta
     8701 gataaaatca aggcgttaat tccaaaagag attttgacta atgaaaatgt tggagaagtt
     8761 gtgactgaag atggatatgc taagcccgag tatgatgagg tatttaaatc attgttcact
     8821 ttaaataatg aatatgattt acttgtaaat tttataaatg atattctaaa agatgctcca
     8881 tatgctaaca ggaatatcaa accgttcact caaataaaga gaataattag agttgaaact
     8941 gatccaacaa ttaattacat tggagagaaa agacctagac ttgattaaaa aatagctacg
     9001 cacacttgtg actttagtca tgagttagta gctaaaatag tcagcatata gggaaactta
     9061 tat
//
LOCUS       DAGQWR010000156         9047 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.157, whole genome shotgun sequence.
ACCESSION   DAGQWR010000156 DAGQWR010000000
VERSION     DAGQWR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.157"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(355..3462)
                     /locus_tag="MIW95_003296"
     CDS             complement(355..3462)
                     /locus_tag="MIW95_003296"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBY3152596.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(3555..3787)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3962..4489)
                     /locus_tag="MIW95_003297"
     CDS             complement(3962..4489)
                     /locus_tag="MIW95_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBY3152597.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(4524..5309)
                     /locus_tag="MIW95_003298"
     CDS             complement(4524..5309)
                     /locus_tag="MIW95_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBY3152598.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(5319..5576)
                     /locus_tag="MIW95_003299"
     CDS             complement(5319..5576)
                     /locus_tag="MIW95_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBY3152599.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFNILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(5595..6053)
                     /locus_tag="MIW95_003300"
     CDS             complement(5595..6053)
                     /locus_tag="MIW95_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBY3152600.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(6093..6797)
                     /locus_tag="MIW95_003301"
     CDS             complement(6093..6797)
                     /locus_tag="MIW95_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBY3152601.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(6807..7442)
                     /locus_tag="MIW95_003302"
     CDS             complement(6807..7442)
                     /locus_tag="MIW95_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152602.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IPVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            7823..8863
                     /locus_tag="MIW95_003303"
     CDS             7823..8863
                     /locus_tag="MIW95_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY3152603.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGIITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
BASE COUNT         2726 a         1597 c         1022 g         3702 t
ORIGIN      
        1 tcacgagtgt gcgtagctat tttttaatca atacttaact ttttgttttc accatacatc
       61 aaaacgcacc cctttgcata ataaataaac caccctgcag aacacacgat aaatttataa
      121 ataaatttat cagaagtagc ttactaaaaa tattattcat cctcacttaa acgtttattc
      181 attaatattc tatcacatga cataaataat ttatatgatt tatatagatt tatttattgt
      241 aaattttgga atattaaaac attttattga atagataatt atattacata aaaaatccct
      301 atagaaatct ataaactaat ttctataggg attttggtta actaactatt ctttttattt
      361 ttttataact tttatacctg tcatatggtc atttaagttt tgttcctcta aagatttatc
      421 atctactctg tttatttcta aagctaaagt ttcagacttg atatattcat taaaatggtc
      481 aacagcctta gcaatttcat catcaccaca gtattctatt actatattat ctaatacttc
      541 aaagttatta gattttctta attgctgtac tttagatata aattctctag cataaccttc
      601 attaatcaac tcttcagtta gcttagtatc tagtataaca aaaagattgt tttcaacaga
      661 tacattaaat ccttccttag cagttatacc tattaatact aaatccttat taaattcaaa
      721 agcttcgcca tctaaatcaa ctgttaaagt ctcacctttt tcaagtttat tagcagtttc
      781 atgagcattt aatttactta atgctccaac gaataaattc atcttagaac ctaataatgg
      841 ccctgcttcc ttaaagtttg gttttaaact aaagttcata tactcatcta aatccttagc
      901 aaatattacc tctttaacat taagctcttc ttttattaaa tcaactacat cacttattgt
      961 atcttcaaac ttaccatcta ctaacacttt ttgtaatggt tgacgaactt ttattctttc
     1021 tacttctctt gaagctctac ctaaagtaac taagttttta gctaaatcca ttttttcttc
     1081 taatttagta tctactaact cttcattaca tttaggatat tcagctaaat gaacagatac
     1141 ttctcctgtt aagtttctat acatttcttc tgacatatat ggtgcaaatg gagctatagc
     1201 tctacaaaga ccatgtaaca attcataagt tgtattgtac actgcttttt tatcttctgt
     1261 taattcagtt gcccagaatc ttcttcttga acgtcttata taccagttag ataaatcatc
     1321 atttataaaa tcttgtatca tccttaaagt tttatttaac tcaaatatag ctaaattttc
     1381 ttctacatct ttcattaaat tattgaactt agataatatc catctatcaa gttctggtct
     1441 atctttatat tctacaaaaa attcagttgg atttatatca tctgtgtttg catataaagt
     1501 aaagaagtta tatacattct tcatagtacc tataaattta ctttggattt cttttaatcc
     1561 atccatatca aatttagttg gagtccatgg tggagaaaca tatagtaaat accatcttaa
     1621 tgcatctgct ccatattggt caaacaattc cattggattt actgtatttc ctcttgattt
     1681 actcattttc ttaccatctt tatcaagtac aaggtctgga actaaaacac tcttatatgg
     1741 tgcttttcct aatacaaatg ttgatactgc aagtaatgag tagaaccaac ctctagtctg
     1801 gtctatacct tctgatatat agtcagctgg gaacaattgg tcaaaatttt ccttattttc
     1861 aaatggatag tggtgttgag caaatggcat agccccacta tcaaaccaac agtctataac
     1921 ctctgttact cttgtcattg gttttccaca tttttcacat tttaagtgta tatcatctac
     1981 atatggtcta tgaagttcaa ctgtttctgg atttacatct tctatagctt tttcagctaa
     2041 ttcagctctt gaaccaactg aatctgtatg accacattca catctccata taggaagtgg
     2101 agttccccaa tatctagttc ttgatattgc ccagtcattt agattttcta accagtttcc
     2161 aaatctcttc tctcctacaa aacttgggaa ccattttaca ccattattat tttctattaa
     2221 tttatcttta agtttagtca tctctatata ccagcttggt tttgcatagt atactaatgg
     2281 agtttgacat ctccagcagt gtgggtaatt atgaactact ttttctttgc tatataattt
     2341 attttcagaa gctagccatt ttattatttc aacatctagc ccatcttcca ttacaaatct
     2401 accttcccaa ggagtagatg taaatttacc agtttcatct actggttgaa taactggtaa
     2461 atcatatctt ttacctgtgt tgtagtcatc ttcaccaaat gctggtgctg tatgaactat
     2521 accagttcca tcttcagtag ttacataatc tcctactgta ataaaaaatg cttttttgtc
     2581 aacttctaca aaaggcatta attgttcata ttctaaacct tctaggtctt tacctttcat
     2641 ttcttctaaa acttcataat cttcacctag tactttatca gctaaaggtt tagatacata
     2701 atatacctca tcaccttttt taacttttaa ataatctaca tcagcattta ctgttaatga
     2761 aacatttgat ggtaaagtcc atggagttgt tgtccatgct aagaagtatt catcagcatc
     2821 ttttttctta aatttagcta taactgtatt agtttttact tctttatatc cttgtgcaac
     2881 ctcatgacta gcaagaccag ttccacatct tgggcaataa ggaagtattt tatgcccttc
     2941 atatatatat ccttctttgt tgaatttgtt tagtatccac catacagact ctatatagtt
     3001 attatctaaa gttatatatg gattatctaa atcaacttca taagccattc tctcagtcat
     3061 tattctccat tgtttttcaa atgagaaaac tgactctcta catttttggt taaattcagc
     3121 tattccatat tcttctattt gttgcttgct agtaagacct aactctttct ctacttgtat
     3181 ttcaactggt aatccatgtg tatcccaacc agcttttctc tttacttgat aaccagacat
     3241 tgttttatat ctacaaactg aatctttaag cgttctagat aaaacatggt gtactccagg
     3301 atttccatta gctgttggag gtccttcata gaatacgaaa cttgggtcat ctttcccttt
     3361 ttctaaagtt ttttcaagta tatttatgtc tttccaataa tcaaaaactt gtgcttctgc
     3421 ttgttttaca gaactgtcta ccaatggctt aaatttcgcc atgtaaatca ccctttccta
     3481 ctaaatatta taattccaaa attttttatt ctttcacata aatttaaata ctaaaaaact
     3541 cgcccccttt aaaccaaagg gacgagttat aaatcgcgtt accaccctaa ttctatatgt
     3601 aaactaaata ctagctacat ataacactct aatagtataa cggtactaac cggcacagct
     3661 tagtagaatt taatcgttca gcctgcagct caagagtgat ttttatcttt ataaattcat
     3721 tggttctcag catacccaat tctctgtaaa attatctaaa gataaatgtc tctatcaacg
     3781 cttttaaaaa tttatctgtg tttatatgaa attttataac aaataatttg aatagtcaat
     3841 atatttttga aaaatataat aatttctatt tttcttacat ttttatccta tttttaatct
     3901 ttgttataga tttctcaaac tcacacagtt attctacata aaaaataaac aaattacaag
     3961 cttattctaa agttgtagca gcttcatcat tactataatt atacactgtt gttccttcaa
     4021 atgcatttac acatttttca tctacatcat aaaaaatctc atcaacactt cttatttcat
     4081 cttctaacag tgatttaaat ttatttctaa atatcttaaa ttctttgact aaagcatcat
     4141 actcttttct tatctctatg acacggttat tagcttgttc tataatttgt ctagctttta
     4201 aatcagcttc ttcaacaatt atcttagcct ttttattagc cgcgctacaa gtgtcttctg
     4261 ctgcactttg agcagttatt aaagtagctt ttagtgtttc ttcaatattt tcatacttgt
     4321 ttacttggtc ttgatataac cctaattttt ctttaagagc tgtattttcc ctacatagag
     4381 attcataatc ttccttgatg atatctaaaa attcatcaac ctcttcatct ctataccctc
     4441 ttaaaccttt tttaaactct ttattttcta tctcaattgg agttagcatt acatttcctc
     4501 ccagctataa tttaaattag tccttataca attaatcttg cttgaacttt tatttttcct
     4561 tttttggtaa tatctcctat ttgtgttacc ttagctcttc ctttgcctct aatagaaatc
     4621 aaatcatcat tttttactat tttagaagtt gaagttatct tttcataatt gacatggact
     4681 ttttctccat ttatgtattt tgcactatct tgtctagata tattgtaaat accacttatc
     4741 acacaatcta acctatctga agatactgta aatgatactt ctttatattt tgaagaacta
     4801 ttaacaatat tttctctgga aatttccttt acaattacat tatttcttga aaccttttct
     4861 aagtttacaa ttataaaatc gcaaatatca ctacttacaa ctacttgaca aaagctatca
     4921 tgtataatta tatctccaat tttttctctt tttataccta gacccagtat tgaccctaag
     4981 taatctttat gagatatatt tttaaacttg aaattccctt ctatttgtaa aaatctaaga
     5041 gtatcttcta tatcttcata ttccatgtaa aatggataaa taaatacagt gcttctttca
     5101 gcttgttcat aacctccatc cacactgtat tttatttcat ttgtagaatt taatattgct
     5161 acagcatttt tcacttcata tggattaaga aactccgtag acttaacatc atagtttttt
     5221 atacaactat tagccttgtc tatcacttta aacattttat ttttcaagtc tatatccttt
     5281 atgtgatttg ttagttttaa tttatccatc ctatcacctt agaatataac caatactaat
     5341 tgtcttagga acatcaataa tactattgca attaaaggag aaaaatctat aggtccattt
     5401 gtatatttat acattataga ccttattggc ccttctattg gctctgtaag attttccaaa
     5461 atattaaaaa ttttactttg tcttccattt ggaaaccaag tcatcaagct ttttataact
     5521 atcatccatg ctattatatc taaaagataa taaagtgcaa ttcttattgt tcccatatat
     5581 taaccgcctt tttactattt attttgccaa gggaagaatg ctttactttc taattctttt
     5641 ttcatatttg catctatatc cacattatta ggagctagta taaatattga tttagaaacc
     5701 ttttgtatac ttccatctaa tacataaact gccccattca taaagttaaa tatagattta
     5761 cgaacttcag aattagtcaa tccttccaaa ttcactataa ctgcccttct ttgttttaga
     5821 tggtctgcta tagttgcagc ttcatcataa accttaggtt caactattac aactttcatt
     5881 tggctagttg tatgtaaatt tacaatttta tttgcttttg cagtactaaa gccactgttc
     5941 atttcttctt cttgaactat atcgtccatg ccactttcat attcatcttc aatataatct
     6001 tcttcttcat caactatcca attcttaaat ttagatatta ttccatttga cattattaac
     6061 ttcctcctta aaatttattc aaatttgagt aattattttt tattgtagtt tctttctcca
     6121 aagattgatg ttccaactct aacaatggta gccccttcct ctatagcaat ttcataatcg
     6181 tgactcattc ccattgacaa aacatccatc cctacattag ggatgtttag ttcttttatt
     6241 tgttttgata aatctttaag acctttaaat acacctctta tctcatcttc atcttctata
     6301 aaaggtgcca ttgtcattag accctttatt tttatatttt tatatttttc tgacacttct
     6361 ttgacaaaat ctacaacttc ttctctatca agtccatgct tagactcttc tttagatata
     6421 ttcacttgta ctaagcaatc tatttctctg tttatttgtt ctgcacgttt ttgaatttct
     6481 tcgcataatg caattctatc taaagagtgt atcatacaaa ctttatcgat tatgtactta
     6541 actttattgg tttgcaaagt tcctatcagg tgccagttta atttgtcacc tataatatca
     6601 tactttctag ctaattcttg gggtttattt tcaccaacag aagtgattcc ttccctcatt
     6661 gcctcattaa ctaagtctac atcaacagtc tttgtgactg ccaaaagggt aatctctttg
     6721 ctgtctctat ctactttttt tgcagcttta tcaattttgt ttcttatctc agacaaatta
     6781 tctttaatag aacccattat tatcacctac tttatcctta tatttgtgtt tatactgtta
     6841 ggtcttaaaa ttattttgtc atatagatta acacttgtaa ctccatttat gtcattttta
     6901 taataatcca caaatacaga agattcattc tcataagtta ttccttctaa tatgacaaat
     6961 tttacacttt gtgattgttc atcaattaca tatacaccac gtttattgtc ttttgttttt
     7021 atagaagttt taggtatttt taaacattcc atctgattat atattatatc aaattcttcg
     7081 acacttgtat cctgatttgc aacaatttgg ttacttattt ttaattttac cattatttta
     7141 ttattatttt catatatttt gtaaactttt gcatctaatt tttgctcatg atattcaatt
     7201 attacatttt gatttttttc aaagttatta gaatcttctt tactgatgta tgtagataga
     7261 taattacttt ggtcattggc atctttttct atagttcctt ttacaggaat ctttgcatca
     7321 actttttcat tttttaacac caacgtatct acatttttgc taattatcat tactaatata
     7381 ttaaatacta aatatataag aataccaagt atcactaatc tggtatattt tacctttttc
     7441 aaagactctc cccctataaa attactttca ttataagtac ttcttctaaa aaatgtttat
     7501 tcctttttta taagctaata ttttacgttc taataattat tttttccata ttaaatttat
     7561 tttttatcaa tcttcctata caagactgca ttatttatat ataattgtat tataaagtat
     7621 aagtttaatt tacattttat acattaagct tactttatat gtttttatag ttatttttat
     7681 ttttagatgc aatataaaac caattaagat ttaaaataac ttttgttatg ttgacattta
     7741 tttaggtaat catttacgaa tcgggataac gttatcataa caaaagttta tgtcctaaaa
     7801 aaaatacaag gaggattttc ttatgtcaga aattttatta ggaacaggtt tacttttatt
     7861 ggttctatgt tgtttcacaa tttttagtta caaggcccca tatggtatga aggctatggg
     7921 agctctagca aacgctgcat gtgctacttt cttagtagag gcattcaacc tttctttact
     7981 tggtgatgtt ttaggtatgg aattttttag aagtgttgga gctgctaatg gtagtcttgg
     8041 tggagttgct gctgctatac ttgtgccttt agccttaggt gtatctccag tttatgcagt
     8101 tttaatagga ttatcttgtt tagatttttc tatattatct ggattcgttg ctggatactt
     8161 tgtttctttc ttaattaagt ttatagagaa aaaagtgcca cctggagtgg atttagtggt
     8221 tgtaattctt ctatgtgcac cgctatctcg tcttatagca tcaaatgtag acccattagt
     8281 taatagtaca ctactacaaa ttggacaagt tttgatagaa gctaccaaca ttagccctat
     8341 aataatggga attatactag gaggcataat aacagtagtt ggaacgtctc cacttagttc
     8401 aatggctctt acatctataa ttggtcttac tggtcttcct atggcaatag gagcattagg
     8461 tgcttttggt tcagctttta tgaactcaac actatttgca aaattgaaat taggttctaa
     8521 aaaagatatt atatctgttg ctattgaacc attaacccaa tcagacataa tttcagctaa
     8581 tccaatacct atatattcta ctaatttctt tggaggagcc ttttctggta tcattgtagc
     8641 cttgatgggt cttgtaaaca tgacaccagg tacagctaca cctgtggctg gttttgctat
     8701 aatgtttgct tataatccag ttcaacaagt tcttataaca gctggtttat gtgctttatt
     8761 caatgtaatt gcaggatttt taggttcaaa aatattcagt acctttaaag ttcttactgc
     8821 tggtgaaata cgtggtactg atgtagaaga agaaattgct taaaaaacaa taagatttat
     8881 aaatttatat ctcaagtagt agagttttat attgtaggca attttattaa ttgcaaacgt
     8941 tttatttttt atttatgttt atgattatta tgtgtgttga atatttagat aaatttttat
     9001 ttattatttt tatttagatt tttgtttagt tttttatttg gttgata
//
LOCUS       DAGQWR010000157         9020 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.158, whole genome shotgun sequence.
ACCESSION   DAGQWR010000157 DAGQWR010000000
VERSION     DAGQWR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.158"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            151..492
                     /locus_tag="MIW95_003304"
     CDS             151..492
                     /locus_tag="MIW95_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBY3152604.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            643..1056
                     /locus_tag="MIW95_003305"
     CDS             643..1056
                     /locus_tag="MIW95_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBY3152605.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNLYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1258..1899
                     /locus_tag="MIW95_003306"
     CDS             1258..1899
                     /locus_tag="MIW95_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBY3152606.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1923..3176
                     /locus_tag="MIW95_003307"
     CDS             1923..3176
                     /locus_tag="MIW95_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBY3152607.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEKENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLREIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3222..3872
                     /locus_tag="MIW95_003308"
     CDS             3222..3872
                     /locus_tag="MIW95_003308"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBY3152608.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGVDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3923..4519
                     /locus_tag="MIW95_003309"
     CDS             3923..4519
                     /locus_tag="MIW95_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBY3152609.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4532..5371
                     /locus_tag="MIW95_003310"
     CDS             4532..5371
                     /locus_tag="MIW95_003310"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBY3152610.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFNNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5616..6770
                     /locus_tag="MIW95_003311"
     CDS             5616..6770
                     /locus_tag="MIW95_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBY3152611.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKFYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7076..8773
                     /gene="ade"
                     /locus_tag="MIW95_003312"
     CDS             7076..8773
                     /gene="ade"
                     /locus_tag="MIW95_003312"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBY3152612.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIASLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3698 a          866 c         1587 g         2869 t
ORIGIN      
        1 aaaattaatg ttaaaaaata aacttataca ttaatattaa taataattat ttaaatatct
       61 taagtaaatc agtcttacat atatttccga tagattatat tgataaaaaa cactaaaatt
      121 aaattaataa aagtacttgg agggatggaa atgaaagagg aaaattttat taatgttgaa
      181 tacgaggata aacgtttttc aattaatagt aaagaagttt tgtacataga aaagcaagag
      241 tatgaagtaa agatttatac tgaaagtggt aattatgaag ttggagaaaa tgctccttgt
      301 ataagaaaac tattgaaaga agaaaatttt ttcaaatgca gtaatagtta tattgtaaat
      361 ctagacaagt gtgaatttta tctcagcaat aattgtattt ttgtgaataa taattttata
      421 agaattttag gttcagaaag aaaaaagaaa ttaaaaaaga aattagaaaa attatatgat
      481 tatgaagatt aatattggga ggctgtattt tatacagcct ctttatttat tgattgtatt
      541 attaataaat tttaaattat gataaaatat aaaacaagta acttatttca gaataaaata
      601 ctttttaaaa gaaagggacg agatatggga gattataata aaatgattga attaaagaat
      661 aacttaggta tcagtgaaag aaagttaaaa tatccttgta tctataaaca ttttaaaggg
      721 aaatattatg ctacaatggg tcttagcaag gctattgatg atatagaaaa catatgtgaa
      781 atatatggta aagaaaattt aatccaaaac agaaataaat ataaattagt tattagacat
      841 actgaaagag aagaagatat ctatgtttat agggatttag atggaaacct ttatcataaa
      901 aaagaagaag atactaatga tttagtttta tacaagacct tatatgatga tacaggaata
      961 tttgcaagac ctttggatat gtttttagaa aaagtagata cagataagta tgtcaattca
     1021 attcaagaat acagatttga agaagtatat aaataatata agagaaatat gtttataaat
     1081 ttagaaaaat tctttaacag tacaataatt tgcagaaata caaatgagat ttattgtaaa
     1141 taatatgtta gattagatta taaaatagaa acaaagtaaa gtattatatg atataattaa
     1201 gttttgagaa gtgataattt atatttaata aaaaataggc atatattagg aggacatatg
     1261 ggaaatactt tttatatagt aagacatgga caaacggatt ggaatatatt gggaaaaaca
     1321 caaggtcatg gtaattctga ccttacgcct caaggtatag aacaggcaaa agaactttct
     1381 gaagatattg gcaagtattc aattgattat atttttagta gcgaccttgg gagagcaatg
     1441 caaacagctc aaattttagg agataagctc aatattgaag ttcaaaaaac tgaggcactt
     1501 agagaaatgg gattcggagt ttgggaaggc cttttaataa aagaaattca aaaagattat
     1561 tcagatatat atgcaacatg gagaaatgaa ccacacttag taaatatacc agaaggcgaa
     1621 actcttaaga ttataaagga aagagtagat gcttttataa aggaactaaa tgaaaaatat
     1681 gataataaaa atataattct tgttacacat tcaattacat tgagggttat gttgttgtca
     1741 tttttagaat caggtatgga aaatatatat agaattaagc aagataatac tgctttaaat
     1801 atagtagaat ttaaagatta tggacctgtt atagtaaaaa tgaatgatac aagtcatata
     1861 aaaaatcatg taaagataaa taattctgca ctggagtagg aatcaattat aaggagaaat
     1921 ttatgagtaa agttatagta gttggtggag gagcttctgg aatgatggca gctttgtcag
     1981 cttcaaaaaa caataatgaa gtaatattag ttgagagaaa tggagaatta ggaagaaaac
     2041 ttagggcaac tggtggcggt agatgcaatt ttaccaataa tagagagata gaagattttt
     2101 ttgataaagt tgtcagtaat aaaaaatttc tctatagtag tttctataca tttacaaata
     2161 aagaccttat ttcgtatttt gaaagtagaa atttggaata taagattgaa aaagaaaatg
     2221 accacaaagt atacactaaa aatgataaat ctatagaagt aatagaagta ttaaataaag
     2281 atttactcaa tcacaatgta aagataatgt ataataagaa agttatagat attataactg
     2341 aagaaattgc tctaaaagat gattctaata aggacaagtc aaagtatcta ataaaaggga
     2401 ttattttaga taatggagat aagatactag gagacaaggt tattatatca actggaggag
     2461 tgagttattc aaagacgggt tcagatggtt caatgtataa gattcttaaa aagcatggac
     2521 atacattaaa caaactatat cctgctttag ttccacttac aatagaggaa aaatggatta
     2581 aagatttaca aggaatatcc atgaagaatg tggaaatatc ttgtaagatt aagaaaagga
     2641 aaatatcaaa atctggagac atgctatttg cccattttgg gataacagga ccttgtgttc
     2701 ttataatgtc ttcttatatt aacaagatta ttgaaaagga aaaggtagaa ttaaatatag
     2761 atttcttacc aaatttaagt actgatgaaa tctctagtat aatcagagca tttccaaata
     2821 aaaatgttct aaataatctt aagcaaatac tacctcaaaa tttcttaaga gagatttttt
     2881 ctctattatc tttagttgat aaaaaggcaa gtgatttgtc taaggcagat gaaattagaa
     2941 ttattgagta tataaaaaat atgaagctaa cttgtaatgg gactacaggt ataaacactg
     3001 gtatggttac ttctggtggt atttcagtta aagaaattaa ttcttctact atggaatcta
     3061 aattagttaa gaacttattt tttacaggtg aagtaattga tatagatgca gaaactggtg
     3121 gatataattt acaaatagca ttttcaactg gatatcttgc gggtataagt gtataaatat
     3181 aattttttaa taattaaata tgaataaata gaggtgtata tatgggtaat ttagttatag
     3241 ctgtagatgg tccagcagga gctggaaaaa gtacaatagc caagatagta gcaaaaaagc
     3301 taaatataaa ttatatagat acaggtgcta tgtacagagc agttacttat aagtgtttaa
     3361 aaagtggtgt tgatgtcaat aatgaaaaag aagttattca aatagctgaa aattcggata
     3421 tagattttaa agataacaat atatatttgg acaaagaagt tataaatgaa gaaataagaa
     3481 caatagaagt aagcaataat gtttctaatg tagcaaaaat aaaagaagta agacagttga
     3541 tggttgaagt tcaacgaaaa ataggaatga aaagttcagt aatactagat ggtagagata
     3601 taggttcata tgttttccca gatgcagatt acaaattctt tttagtagct actccagaag
     3661 aaagaggaaa tagacgctat aaagaacttt gtaataaagg atataatact actttagaag
     3721 aagtaattga ggatataata agacgagatg aaattgattc aaatagagaa tttgcaccat
     3781 tggtaaaagc taatgatgca ctagaaatag atactacagg aaaaactata gaagaagttg
     3841 tagaagaagt tgtatctaaa ataaatcttt agttttttat gcatcataat gaaaataaat
     3901 gtagtgttga agggagataa acgtgaattt ttatagattt gtaataaata tttttaaggg
     3961 tttttctaaa atatttttta aatatgaagt tataggtgca gagaatattc cagatagagg
     4021 aaatatagta atagcatcaa atcataaatc gaatcttgac cctatattct tggctgctgc
     4081 catagaaaat agggaaatag ctgcaattgc aaagaaagaa ttgtttaaag taaagccatt
     4141 gggatttata ttaaaaaaat tgcatgttat gcctataaat agagaaaaac cagatgtatc
     4201 tacaataaaa actattttaa ggtcagttag agatggttat gtattaggaa tatttccaga
     4261 gggtactaga ataaaaggag attcttttgg taaggctaag gcaggtcttt ctgtatttac
     4321 aataaaaagt aaatctaaag ttgttccagt atcaataata tctaaatata aactttttag
     4381 taaagtaatt gtatatattg gagaaccaat atcatttgaa gaacatttta aggaaaaatt
     4441 gagtaatgat gaccatgaaa ggatatcaca agagatttta gaagtgataa aacaaaatta
     4501 ctttaaatat tcaaaataag ggggtaaagt tatgaacata aaaatagcaa aaaatgctgg
     4561 cttttgtttt ggagtaaaaa gagcaatgaa aatggcatgg gatgaagttg aaaaaaatga
     4621 ttctggcata tatgctttag gtcctcttat tcataacaag caagcagtag caaaatatga
     4681 agaaaaaggt cttaagactg tgaatgaaat tgatacaata ccaaatcatg aaaatatgat
     4741 aataaggtct catggagttc ctgaaaatat atataaggaa gccaaagata aaaaattaaa
     4801 aattgtggat actacatgtc catttgtaaa gaaaatacat acagtagtaa gtgaatatca
     4861 taataaaggc tatgaaataa tagttattgg agatatgaag catccagaag ttataggtat
     4921 aaatggatgg tgtgaaaatt cagctataat aataaagacc ttagaacaaa tggaaaatat
     4981 ggaatttaat aactccaaaa aatattgtct tgttgcacag actacaataa atccagagtt
     5041 gtatataagt atagttaata aactatcaga taaattagaa gaaatagtat ttaatgatac
     5101 aatttgttca gctacaaaaa caagacaaga atcagctaaa gagttagcta aagaagttga
     5161 ttgtatgata gttataggag gtaaacacag ttcaaatact caaaagttag taaaggtttg
     5221 tgaagactta gtaccaactt ttgcaataga gactaaagat gaattggatg taaatacgct
     5281 taaaaaatat aaaaatttgg gtattacagc aggggcatct acacctaatt ggataataga
     5341 agaagttgta acattccttg aaaatttata atttgatagg taataaaaat aggtggacat
     5401 tgttataatt tatcataata tccacctttg atttcatatt acatgtggta aatagataac
     5461 ctaatatcaa aaagtgtgta tcttaagatg aaaaactaag agtataagat gcgcactttt
     5521 taatattaaa aattcaaaag aaatattgat attgattatc aatagaaaat agaatataat
     5581 ataattgttg caaaattagt ctaggaggta aattaatgga aatgttgata tccgcttctg
     5641 aagaggcgtt tttacatgta ggttctatga ttggattttt tattttgtta tttggatata
     5701 ttaattataa aactagtgga aattttacta atatcatatc aaaaaacaga aaatttcaac
     5761 ccttaatagg tgctttaatt ggagctatac caggttgtgg cggaagtctg gcaataatgc
     5821 cattgtatat aaatggaaaa ttaagctttg gagcaataat tgcaagttta atagctagta
     5881 tgggagatgc agcatttgta cttatttcat caaatattaa aatgtatttt tttgtcacta
     5941 tagttagtac aattacagga ataataacgg gacaattagt ggattacttt aaactagaag
     6001 aaaaattagg gttaaaaaac cgtagaaaat cagataaata tgatataagt aaaaataacc
     6061 ctaaagaaaa taaagaacaa cataatcatg atgaaataat tttagacact ctagctaaat
     6121 ctcatggaaa tacaaataga cttgcattta taataacaca tggaaaggga tataaatttt
     6181 atataggaat tatagtaatc ggttttatat ttatgtcact tgctcactct ggacttaatc
     6241 ttcctataat tgagaagtta cattcattgg aagaagtaat agcagtgatt ggaatattat
     6301 tttcaataat ttatatgtgg tgttttaaaa aggtatttaa aaattctaat caaaaagagg
     6361 aagaaaataa aaagatttct ttgcgagaaa tgcttatcca ttctgttgga gaaatatcat
     6421 ttgtaattac atggattttt attgcatatc ttatttacga tttgataata ttaattttag
     6481 gtggagatga atacttagtt aaactagtat tatcaacagg tgtaataagt gtatttatag
     6541 gtgcaggtct tggacttatt ccaggttgtg ggatacaaat tgtgcttatg tcattttatt
     6601 tgaagggaac tataccactt ggtgcagtga tagccaactc tatttctcaa gatggagatg
     6661 cacttttccc acttttagca atggataaaa aatcttcttt atgggctatg attattacta
     6721 ctataccagc aatacttgta ggtatgatag tttatgcatt tttaggttaa atataattac
     6781 gaataaagaa gtattaaaaa aataataaag cacataaatt tatttataag aatagtgaag
     6841 attaaatata aaaataaaaa attcaatatg aattaaatta ttgcttaaat attaatgaga
     6901 acatgggaga taatattatt cctcatgttc tctctattta attaaaggaa ttatggaaca
     6961 aatttcaagt gatactttct atcaaaactc atatataata tatattaata aattaaacta
     7021 taatacgtaa tacagccagt atattgatat aaaaaactta ggaggagttg taaaaatggg
     7081 taatttaaaa agacagtgtg atgtttcaag tggaagagaa aaagctgaca ttgtactaaa
     7141 aaatgggaca atcataaatg tatttacaga agagttaata acaggagatg tagcaatagt
     7201 aggagacact atcgttggca ttggcgatta caagggcaat gtagaaattg attgtagcaa
     7261 taaatatata tcacctggct ttattgatgc acatatgcat attgaatcta caatggtaat
     7321 gccaattgaa ttatcgaaaa aattattaaa atcaggtacg acaactatca tagccgaccc
     7381 acatgagttg gtcaatgtaa aaggagctag tgcaatagat tttttattag aatcgacaaa
     7441 agatatacca cttaatgtat atattatggt gccttcatcc gtacctgcaa caagttttga
     7501 aactaatgga gttggtaaat ttagtgcaaa agacatggag agttatgtaa ataatcctag
     7561 aattttaggt ttaggagagg taatgtgttt taatgatgtt ataaattctg aaaatgaaat
     7621 cttggataaa ctagagcttt ttaaaaataa agttgtagat ggacatgcac ctaatataaa
     7681 tggtaaatca cttcagacat atgtatgtgc aggaatagaa aatgaccatg aatgtataac
     7741 ttttgatgaa gtatatgaaa aattgagagc aggattaaaa attttaataa gagaaggaag
     7801 cgcagcaaaa aatttaaagt ctatagtaag tggtatgcta aagcataact tgccaattga
     7861 agaatttatg ttttgcacag atgataagca cttagatgat atagaaaaac aaggtcacat
     7921 aagatggaat ataaaatgtg cgattgattt aggtatggaa cctgttaggg ctataaaagt
     7981 tgcaacttat aattctgcaa aagcatatgg gttaaggaaa attggagcaa taggtgcggg
     8041 ttataaggca gatattgttg tattgaatga tttagacaaa atggaagtgg atagtgtata
     8101 taaagatggt aatttggtaa acgaagagat gttttctaat tacaattatg agattaaaga
     8161 taaagaattg cttaatacag tcaagtttaa gtatattaat aaagaaaaaa tacagttaaa
     8221 agtaagtgaa aaaaattatg taatggagat tgtaccttat caaattttga caaataaggt
     8281 atatgaatct ttaccatgtg cagatggata ttttgttcca aataaagagt attcaaaact
     8341 ttgtgttgtt gagagacata gaatgactgg aaatgtagca attgcttcac taaaaggatt
     8401 tggcataaaa aatggagcaa ttgctactac tgtggcccat gattctcata atattatagt
     8461 tgcaggggat aatgatgatg atattctagt agctataaat tacttaaagg aaatacaagg
     8521 tggttatgta attgtatcaa atggaaaagt tcttgcacac ctaagtcttc aagtagctgg
     8581 attaattagt acttttacag ctgaagaagt acaagaaatt actgataata tgttggagat
     8641 agcaagaaaa atgggagttc cagagtatgt agaccctttt attacacttt cttttatggc
     8701 acttccagtt gttccacaaa taagacttac agatttaggt ctatttgatg ttgaggaatt
     8761 taaatttata tagataatat aaaagtaata ataaatgagg ctttctcaaa atagggataa
     8821 gtctcttttt attgttgaaa tgatgaaaat atatttgatt tcatacaaaa aagagcatct
     8881 aatgaactaa aaattttatt tttagatatc cttattttaa ttaatctatc atttaacctt
     8941 gttttaagca tagatatctt aataaaacta ttttcagtct attttagaat ataaatacat
     9001 gaaaattata tatttatgaa
//
LOCUS       DAGQWR010000158         8800 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.159, whole genome shotgun sequence.
ACCESSION   DAGQWR010000158 DAGQWR010000000
VERSION     DAGQWR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.159"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            332..1348
                     /gene="gap"
                     /locus_tag="MIW95_003313"
     CDS             332..1348
                     /gene="gap"
                     /locus_tag="MIW95_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBY3152613.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYSDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNQKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            1743..2912
                     /locus_tag="MIW95_003314"
     CDS             1743..2912
                     /locus_tag="MIW95_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152614.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            3293..7546
                     /locus_tag="MIW95_003315"
     CDS             3293..7546
                     /locus_tag="MIW95_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBY3152615.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKISYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            7695..8189
                     /locus_tag="MIW95_003316"
     CDS             7695..8189
                     /locus_tag="MIW95_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBY3152616.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSVYIGDEKFNNLKNECIKK
                     GIDIKISKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKNIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            8305..8682
                     /locus_tag="MIW95_003317"
     CDS             8305..8682
                     /locus_tag="MIW95_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBY3152617.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         3562 a          915 c         1604 g         2719 t
ORIGIN      
        1 ctaaataaaa atttgttttt attattttaa attagaggag attcttgaag atatattatt
       61 tttaaaaata agtatagttt aaaaattcaa aaaaaagatg gcaaaatatg tcccaaggga
      121 cattgagtaa aataaatgat ttcagcttat ttacactaaa aagtacatac taaatgaggg
      181 gtgaaataaa gaaaaatgat atactaattg tcttttgata ataaaaaaac atggaatttg
      241 attatttaat caagaaaaag tattgaacaa atgatttatt tagtgtacaa taatcatgta
      301 aaaacatagt aatggaggga gacatttcat aatgaaaatt aaggtaggaa tgaacggatt
      361 tggaagaata ggaagagctg tattaagaat agctcaagaa gaattaggag ataatataga
      421 aattgtagcc ataaatgcaa gagcaacaac agaatctttg gcacatttat ttacatatga
      481 ttcatgctat ggaactttta gaggtgaagt agaggcaaag gatgaagaca ctctagtagt
      541 aaactctaaa gagataaaaa tattaagata tagtgaccca gaagaattac catggaaaga
      601 actgggggtt gatatagtaa tagaatcaac aggattattt acacaaagag aaaaggcaga
      661 aaaacatatt aaagcaggag caaaaaaagt aattataaca gctccaggaa aaaatgaaga
      721 tataactata gttatcggag ttaatgagaa agaatatgat aatcaaaagc acaatataat
      781 ttcaaatgca tcttgtacta caaattgtct agctcctttt gctaaagtat tggacgaaaa
      841 atttggtata gtaaaagggt taatgactac agtacattca tatactaacg atcaaagaat
      901 attagataaa agccataaag atttgagaag agcaagagca gcggctgaat ctataatacc
      961 taccactaca ggagcagcaa aagctgtgtc aagagtacta ccacaattag aaggaaaatt
     1021 aaatggtttt tcacttagag taccaacacc aacagtttca cttgttgatt tagtgtgcga
     1081 acttaaagaa aacgttacat ctgagcaagt taactctgtg ttaaaagatg ctgctgaagg
     1141 agaattaaaa ggtgttttag gatattgtga caagccactt gtttctatag attataaagg
     1201 agattcaaga tcatctatta tagacgctct atccacaatg gttatagaag acaatatggt
     1261 taaggtagtt tcttggtatg ataatgaatg gggatattct tcaagaactg ttgacttagt
     1321 aaaatatgta gctgaaaaat taaaataata tatatttctt aaagcaaatt ttaaaaaaat
     1381 tttttgaaaa aattatataa aactaaaaaa aaagattatg aaaaatattg aaggaaaaat
     1441 tttttcataa tcttttttct ttcatgcaaa aagatacaag ttacaactta tatttgcata
     1501 ttaggtcgtt agagaagtat atatatattt agtaaggaat catacttttt ataaaatgaa
     1561 agataagatg tcgaaaactt gcataaatct gaaaaatttt tattattaaa atactgattt
     1621 tcattttaaa aatgataaaa aaatgacaaa taaagttgca aatagtaata cttataagta
     1681 taattaaaaa agagaaaagg ttttgttaaa ctttataaca aaaaaagaag ggatgattca
     1741 aaatggagaa gttaacagac aggagacagg ttaaggcagt aagcatggaa accttcattt
     1801 tcctaatatt actagtagtt ggttttgggt atgttggtag tattatggga gcaggaatga
     1861 tgttcaaagt aataatgagt acagcacatg ctttactatt ggagacagtt ttcttaatta
     1921 tggctatggc agttcttgca ggcgcattaa gcgcactact ttcagagttt ggagtaatag
     1981 cgttaattaa taaaattttc gctgtattta tgaagccttt atatggacta cctggtgcta
     2041 gtatagcagg agctataaca acttatttat cagataatcc agctattata ccttttgcta
     2101 aagataaaac attcacacag tattttaaac aataccaagt tccagcatta tgtaacttag
     2161 gaacagcctt tggtatggga cttatcttaa caacatttat gatttctcaa ggtacagaat
     2221 atgtacttcc agcattaatt ggtaatttag gtgcaattat aggtagcgtc ataagtgtta
     2281 gaataatgtt gacatttact aagaagtttt ataaatacaa tccggaagaa gataaagcaa
     2341 caggaacatt agagaaaaag gatgaattta gagaaatcag agaaggaaat gtatttcaaa
     2401 gagctttaga tgcaattctt gaaggtggta aaatgggggt agatatggga atggctataa
     2461 ttcctggagt cctagtagta tgtacattgg tgatgttatt aacttttgga ccatctacag
     2521 accctgttac aggtcaagaa gtttatacag gagctgcata tgaaggaata aaattacttc
     2581 cagttattgg agataaactt ggatttatat tagaaccatt atttggattt acatcaccag
     2641 aggcaatagc attccctata acttcattgg gagctgttgg tgcagcaatg tctctagttc
     2701 cagagtttat aaagtcagga gctataactc ctaatgatat agcagtattt acagctatgg
     2761 gtatgtgttg gagtggatat ttaagtacac atattggtat gatggatgca ttaaatgcta
     2821 gacagttggc aggtaaagct attctttctc atacaatagg tggtttatgt gcaggagcag
     2881 cagctcactt tatatttaca ctcgtaggat aaacgtaatc agctaagata aaaaataata
     2941 tacaaaaaaa tggttgttga tatgtaataa gaatttacat atcaacaacc atttttttct
     3001 gactaaagat aatttataat acataaacat taagtggata ttagacttaa gttatattta
     3061 tgtaaaaagt aacaaaaatc tgtagaaatt tatgacaaaa tttagtacac taactatatg
     3121 ttcttattta aaatttaagg ttagaattag gaattaccta gtagagtaag ctttgattaa
     3181 taaaagaatc ataaaataaa aatattagtc ataaagatta taaataactt acttaaaata
     3241 aagccataga acttacaaaa attaaaaaag catataaata tggaggggta caatggatga
     3301 attatttcat ataggaattg atgtaggttc tacaacggtg aaggttgtgg tacttaataa
     3361 tagtaataat atagttcata aagaatatag aagacattat tctgatgtaa aaaaatcagt
     3421 taaagaagta ctaaatggta tatatgaaaa attgggagat ataaatacta ccattataat
     3481 aactggttca ggtggaattg gaatatcaaa aaaattaggt gtaaaatttg ttcaggaagt
     3541 tatatctagt acaaaatcaa tagagtattt tcatcctgaa actgatgttg tcatagaact
     3601 tgggggtgaa gatgcaaaga taacatatct aagtggagga atagaccaaa gaatgaatgg
     3661 aatatgtgca ggtggcactg gagcattcat agaccaaatg gcatcacttt taaagacaga
     3721 tgccagtgga ttaaatgaac ttgctaaagg gtataatgtt atatatccta tagcttcaag
     3781 atgtggtgtt tttgctaaaa cagatattca acctcttata aatgatggag ccaatcagac
     3841 tgatatagca atgagtattt tcaatgcagt tgtcgtacaa actgtgagtg tcctttcttg
     3901 tggaagaaaa atagaaggaa atgttgcgtt tttgggtggt ccattgtact ttttatctga
     3961 acttagagaa gcatttaaaa gagtattgga tttaaaagat gaaaacataa tcttcccaga
     4021 aaatgcacaa ctttatattg ctattggagc aggattatta tcagtagatg aagataatat
     4081 acaactaaaa tctctaatag aaaaattgga tagtataaag aatattgaag atggagaagt
     4141 aaatttatta gaacctttat ttaaagataa aaatgagtat gaagaatttt caagaagaca
     4201 tgaaaaagaa aaaataagtt atgtggacat aaattcaata aaaagcaatt gttatttagg
     4261 aattgatgca ggctcaacta caactaaagc agcacttatt gatgaggatg gaagacttgt
     4321 atattcttat tataatagca atgaaggaaa tccattaaaa acaaccatta aagttataaa
     4381 tgaagtatat gatatattac caaaaaatat taaaatattg agttcaactg taactggata
     4441 tggagaaggt cttattaaaa aagctcttaa aatcgataac ggagagatag agactatagc
     4501 acattacaaa gctgctaaat ttttcaataa agatgtagat tttatacttg atattggtgg
     4561 tcaagatatg aagtgcctaa aaataaaaga tggagtaatt gataatataa tattaaatga
     4621 ggcttgttca tcaggttgtg gctcattttt agagacattt gcaagttctt tatctatgac
     4681 aatagaagaa tttgcacatg aaggtattta ttctcaaaat cctgtagatt taggttcaag
     4741 atgtactgta tttatgaatt ctagagtaaa acaagctcaa aaagaaggtg caagtgttgg
     4801 agatatatca gcagggctat cttattcagt aataaaaaat gctttattta aagtaataaa
     4861 gattagagat ttaaatgaga ttggtaataa tattgttgta caaggtggaa ccttttataa
     4921 tgatttggtg cttagaagct ttgaaaaact aattggaaaa aatgtaatta gacctaatat
     4981 ttcaggaatt atgggagctt ttggttcagc acttatagct aaagaaaaat atgaaagtgg
     5041 atacaaaaca tccttgttat ctaaggaaga gttgaataat atcaagctag aagcaagtgt
     5101 aactagatgt aaaggttgtt caaatcattg tcttcttaca ataaataaat tttctgataa
     5161 tgaaattttt atttcaggta atagatgtga gaagggtgaa gctatttatg gtgataaaaa
     5221 aataggatta gaaaagaaaa aaccaataaa tttatttaag tacaaataca atagaatttt
     5281 tagatacaaa ccattggaaa aagaagaagc aaaaaatggg gaaataggga taccaagagt
     5341 attaaatatg tatgaagatt atccattttg gtttactttt ttcaatgcta tgggctttag
     5401 agttgtactt tcagataggt catcaaaaca gttatatgaa actggtataa ctagtattgc
     5461 atctgaaaca gtttgttatc caggaaaatt agttcatgga catatagaaa atttaattgc
     5521 aaaaggtata aaaaatatat tttatccaag tgtaacaaat gaaaataaag aggattcaaa
     5581 tgcagataat tattataatt gtcctgttgt aatatcatat tctgaagtaa taaaaaacaa
     5641 tgtagagaat ataagaaata agaacataaa ttatataaat ccatttataa gtctaaatga
     5701 taaggaaaaa ttgaagaaaa gactatatga tgaattatca agacattttt ctgatataaa
     5761 aataactaaa gaagaagtta atcatgcagt tgataaagct actgatgaac agaatttatt
     5821 taaaatggag atacaagcag caggtgaaag agcacttaaa gaaataaaag aaagaaatat
     5881 gaaggggatt gtgttatgtg gaagaccata tcatatagac ccagaaataa atcatggaat
     5941 gccagagctt ataaattcat tagatatggc agtgcttact gaagactcta tatgtcattt
     6001 agccaatgta caaagaccac ttagagttgt agaccaatgg gtatatcatt caagattgta
     6061 taaagcagct agttttgtaa gagataaaaa ctatttggaa cttgttcaac ttaattcatt
     6121 tggctgtgga ttagatgcag taactacaga ccaggttcaa gaaatactta atgaaaaatc
     6181 gaagatatat actattataa agattgacga aggcaacaat ttaggagcag ctaagataag
     6241 gatgaggtcc ttaaaagcag ctatgtttga gagagaaagc aaaaatatag atgtaaaaaa
     6301 tataaaggta aacaggatac agtatgctaa aaataatcag ataacgaaca aacatactat
     6361 attagcacca caaatgtctc caatacattt ccaattcctt gaaaaagctg ttaacttaag
     6421 tggttataat atagaaattc taaaagatac tgatagtagt gtaattgaag aagggttaag
     6481 atatgtaaat aatgatgcat gttatccagc tataatagtt gtaggtcagc taataacagc
     6541 attaaaatct ggcaaatatg atttaaataa tacttctgtt actataactc agactggtgg
     6601 aggttgtaga gctacaaatt atattggatt cttaagaaag gctatatatg atgctggatt
     6661 taaagatgtt cctgtcattg cacttagtgt aaatggaata gaagatagtg gtataatgga
     6721 taatatatca ctaaaattaa tcaatagact ctttatgtct gttgtttatg gtgatttgct
     6781 gatgaaagta ctatatagag taagaccata tgaaaaagtt actggaagtg caaatgctct
     6841 atatgaaaaa tgggttgata tatgcaaatc ttctcttgtt aaagcaaaaa tttctgtatt
     6901 tactagaaat ataaaagata tagtaaaaga atttgataat ttagagatac tagatatcaa
     6961 aaaaccaaaa gtaggtttgg ttggagaaat attggtgaaa ttccatccaa ttgcaaataa
     7021 taatttagta gatatattag agcgagaagg tgctgaagca gtcgtgccag accttacaaa
     7081 tttcttctta agttgtgcct ttaatactat atacaaacac actcatttag aaggaagtag
     7141 aaagagtaga atgataggag aagcatttat atacataact ggaatatatc aaagagtata
     7201 taaaaaagca cttgataaga gtgagagatt ttatgctcca gccaatataa aaaatgttgc
     7261 taaaagtaca gaaccagtag tgtcattagg taatcaaact ggagaaggtt ggcttttaac
     7321 aggtgagatg gttgaattat taaatgaagg tgtagaaaac attatttgta tgcaaccttt
     7381 tggatgttta cctaatcaca taattggtaa gggttctata aaagaattaa agaggttgta
     7441 taaaaatgca aatataatac ctattgatta tgacccttct gcaagtgagg taaatcaact
     7501 taacaggata aaacttatgc tttctaaggc ttttaaaaat atataagatt tatattttga
     7561 tatgttaaaa gttaatgaat ttaaaaatat actctatatt aagtatctat aaaaaagagt
     7621 tatcctattt tttagggtaa ctctttatta gttaaaaaat tatatgtata ctgatattaa
     7681 aggggggaag taatatgagt ttagtatatg atgtttataa atcaaaatta ggaaatttgt
     7741 acatattaag tgaagatggt tttatagtta gtgtatacat aggagatgaa aaatttaata
     7801 atttaaaaaa tgaatgtatt aaaaaaggaa ttgatataaa gataagtaag gaagatttaa
     7861 aggaagcaat aagccagata gatgattact ttaatggaaa aagaaaagtc tttaatttaa
     7921 atattcgtat tgaagggact gaatttcaaa aatcagttta tagggaaatg ttaaaaatac
     7981 cttatggaga aacattgtgt tattcagata tagcaagaaa tataaacaat ccaaaatcag
     8041 taagagcaat aggacaagca agtaaaagta ataaaattcc aataataata ccttgtcata
     8101 gagtagttgg caaaaaaaat attggtggat atatggggaa tcattctgat ttgaaagaga
     8161 tactattaaa tttagaaaga cagagttaac ttagaaagaa atgtttttat tgttaatatt
     8221 tgatataata atagtaaaaa gaagtatttt ttacaaaaac aatgaagaat ttattttaat
     8281 ttacaaaaat gagggggaat tttaatgtgt gataaagtta tagcatcaaa atatcatgag
     8341 aagggattta attgtgcaga atcagttata aaagcatata atgaggaatt taatacagat
     8401 ataccagttt gtttaggaag tggactgggt tcagggtgtg gggttgctag tttatgtggt
     8461 gcagtaaatg catcaaatat aataattgga tacgtaaaag gcagaaacca cgaagatgaa
     8521 agtactaaag ctaaagtata tgctaaagat ttaactacaa cagtaagaaa agagtatggt
     8581 tcagagttat gtatagacct aaaaaaagat ttagttgctt gtagagaaat aatggatttc
     8641 gcatatgata gtctaaagga aactttaaaa aaagaattat aagaaaataa gctatcaagt
     8701 ataaattatt tgatagctta tttttatagt cgatactaaa taaatcaaat atattttaga
     8761 attattattt gtaaataaac agcaaataat tttaggattt
//
LOCUS       DAGQWR010000159         8784 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.160, whole genome shotgun sequence.
ACCESSION   DAGQWR010000159 DAGQWR010000000
VERSION     DAGQWR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.160"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            230..1309
                     /locus_tag="MIW95_003318"
     CDS             230..1309
                     /locus_tag="MIW95_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBY3152618.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDEINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYMKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRRH
                     YPGIHSITIIINGVEKGKLDFELGV"
     gene            1633..2001
                     /locus_tag="MIW95_003319"
     CDS             1633..2001
                     /locus_tag="MIW95_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBY3152619.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2118..2921
                     /locus_tag="MIW95_003320"
     CDS             2118..2921
                     /locus_tag="MIW95_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY3152620.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFMPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPSELNQIMDIFIKQLNI"
     gene            3306..4196
                     /locus_tag="MIW95_003321"
     CDS             3306..4196
                     /locus_tag="MIW95_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY3152621.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSARFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTIQNKDSCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
     gene            4659..5102
                     /locus_tag="MIW95_003322"
     CDS             4659..5102
                     /locus_tag="MIW95_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3152622.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            5274..5444
                     /locus_tag="MIW95_003323"
     CDS             5274..5444
                     /locus_tag="MIW95_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152623.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            5995..6903
                     /locus_tag="MIW95_003324"
     CDS             5995..6903
                     /locus_tag="MIW95_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBY3152624.1"
                     /translation="MTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFINDI
                     LKDAPYANRNIKPFTHIKRIIKAETDPTINYIGEKRPRLYILAEDEESNHINIEMQRA
                     LEDDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLTM
                     DGHPNIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIKR
                     ALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGVEKGKREEKFNIIIKLKNK
                     GYNLSEICDIIDDLNKSEVEKIYNQN"
     gene            7083..7571
                     /locus_tag="MIW95_003325"
     CDS             7083..7571
                     /locus_tag="MIW95_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBY3152625.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            7657..8064
                     /locus_tag="MIW95_003326"
     CDS             7657..8064
                     /locus_tag="MIW95_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152626.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            8065..8283
                     /locus_tag="MIW95_003327"
     CDS             8065..8283
                     /locus_tag="MIW95_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3152627.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
BASE COUNT         3472 a          869 c         1543 g         2900 t
ORIGIN      
        1 agtaaagtat aaaattctat tactataata aaagatagaa ctagaagtct ttatgaaagt
       61 gggaaaagta tttatataaa taaatttata tggtttatag tttaacagta ggaaatatcg
      121 tatgagtgag aatatcgtat tttacataaa gatttagcaa tatgacacac tgttgtcgtg
      181 ttctgtagga tatgattaat aaatcagata tgaaaggaga gtgagtaata tgccagaatt
      241 actaaaaaat atgtataatc gtgagtcact ttatgaagtt gcagtagcta tccaatcagt
      301 ttacaactca tttaaggtag acgagttcat aaaatctaca atggatgaga catggaataa
      361 cttagaatta aaagccagat gtcgaaagat aagtatgagt ttaggaatgt atttaccaga
      421 agactataag gaagctcttt ccattcttga aaaatcagtt acaggttttt attttgcttt
      481 tttctttcca gattttgttg aagtttatgg gcaggacgaa ataaattggg atttatctat
      541 aagtgcatta gaaagaaata ctgagtattg gtcttcagaa tttgctgtaa gagcatttat
      601 catcaaggat gaagagcgta tgatggcaca gatgcgtaaa tggtctaagc ataagagcga
      661 acatgtacga agacttgcaa gtgaaggttg tcgtccccag ttgccttggg gacaagcaat
      721 atccaaattt aaaaaagacc ctacacctgt actgcctata ttggagcaac ttaaaacaga
      781 tacatcaacg tatgtacaaa agagtgttgc taataatcta aatgatatct caaaaacaca
      841 tccagattta gtcataagca ttgcaaagga ttggtacggt aaaaataaat ctactaactg
      901 gattgtaaag catggttgta gaactctact aaaaaaaggc aatcgtgatg tattagccct
      961 ttttggatat gatgatacta cttctataaa tcttcaggac tttactttag aaacaacgtc
     1021 tatttctatt ggagaggatt taactttttc atttaatatt ttagcaaaaa aagcgacaaa
     1081 aacaaggctc gagtacggaa ttgattatat gaaatctaat ggaaaaagga ataggaagat
     1141 atttaaaata tcggaggttt cattaaaaga aaatgagaaa aagtcatata tgaaaaaaca
     1201 ttcatttgca gatgtaagtg taaggagaca ttatccaggt atccactcga ttactattat
     1261 tattaatggg gtagaaaaag gcaaacttga ttttgaatta ggtgtataat tttactaaca
     1321 gcataaccta tatcagatac tttgctaaac ctatatcgta tactctaata aatctatatt
     1381 aggtagttta ctacttgttt actgaattta aatacaaaga aatacaggtg ttaatccctg
     1441 cccattaaag gaatatgagc ataacacaaa agtagaactt atcaattctg ttacgggtat
     1501 agtagtaaat ccatagaatc acaattagaa gatgaagtgt aaaaaataag ttagtgttga
     1561 ttttagttgc tagtgatatt gaatagttat acaatagcca tataaattag aatttgtaga
     1621 ggagaaatgt aaatggatgt atatagtttt tggaatgaca ctttggcaca agatgctgtt
     1681 aagataagaa aatattttca tgaagatgct tatattaatt ggcatgatac aaatgagcat
     1741 tttactttag aagaatttat tattgcaaat tgtgagtatc ctgataaatg gaatggtaaa
     1801 gtagaaaggg tgctagaatt tggaaattta gtaattacag tgacacatgt ttatgcaata
     1861 aataaaccac tatcatttca tgtcgtttca tttatcagcc ttaaagatga taagattatt
     1921 tctattgatg aatattgggg agaggatgat gatgtgccac agtggagatt agataaacat
     1981 attggaaagc caataaaata gtgtttgttc tgttttataa ctgaaatttt gataacattt
     2041 gatattttga tttgtcattg gtcaacatag tcgttagtgt aataatttaa aaaagtagaa
     2101 tttggagagt gagcactatg gaagaaaaat acatcttatc tgaccgaggt aaagtatatt
     2161 attggataaa agaaagtaat agtgtgtttg caaagaactt aatttttttg ccaggactaa
     2221 cagcaaatca tcatcttttt gatttccaga tacagtactt ttcaaaaaat cataatgtat
     2281 tggtatggga cgctcctgct catggaaaat caagacctta cttagatttc tcatattcta
     2341 acttggtaga agaattgaaa gtaattttag ataaacaaaa tatgaaaaag gttgtactta
     2401 tagggcaatc ggcaggggga tttgtggcac agtcctttat tgtaaaatat ccaaatatgg
     2461 tagaaggatt aatgatgatt ggaacttgtc catatggaac tgactattat agtaagtcag
     2521 atattttctg gttaaaacag actgaatgga tgttaaagtt attcccagat aaattgttgc
     2581 gaaacataat atctaagatg tgtggagcta cgtctgaagg cagaaggagt atgatgaaaa
     2641 tgttagaagg ctacgaaaaa aaagaactgt gtcatctcat gtatctggga tttgctgggt
     2701 tcatgccaga aattcaagac ttaaatatac attgtccagt ttgtttggtg gttggagata
     2761 aagacaagac gggtaaagtg agaaaatata atgagcagtg gcacaataag actgggtatc
     2821 cattgcacat tataaaaaat gcttcacata atgtaaatgt cgataaacct agtgaactaa
     2881 atcagattat ggatatattt attaagcaat taaatatata aaaactacat actcattagg
     2941 taaattatgt agtatatgtg tttacatatg gttatagaaa taaatatgta ataaactact
     3001 taacacttaa aaagtggatt tgtccagagc ataatacaaa gcatgatata taaattttta
     3061 agcgaaatac atcaaaagaa gtattagaat tagtggagat aaaacagcta ttagagatta
     3121 taaataaata gttttaaagg tactacgaat atagaggaag gtaaatcata cgttattcaa
     3181 ggattataaa tgagtagttt aaaagtacta tcggataggt ctctgcatat ttaaacctta
     3241 gtaaaggtag ttaaagtagt agtttaaaaa ctaaaaaatt atataaagga attaggtata
     3301 agaaaatgat tgagagtata ccagcaaaac aaattttaca aaaagtgaaa tttgacaata
     3361 caagatggtt tggaattgac tataatatga atctctatcg tggttgttct catggatgta
     3421 tatattgtga tagccgtagc acaatataca acatagagaa ttttgacaaa gttagatata
     3481 aagaaaatgt gatagagatt ttaagtaaag aattgcgttc aaaaagaaaa aaaggggtag
     3541 taggcattgg agctatgtct gacacatata atccttttga aaagcaactt tgtattacaa
     3601 agcaagcact agatttgatt tcggaaaatc attttggagt gtctattgat actaaaagta
     3661 gtttggtggt tagagatata ccgattttgc agaaaattaa aaaaaataat tctgctattg
     3721 taaagttgac tataaccact gcaaatgatg aattatcaaa gaaaatagaa ccttatgtaa
     3781 atccaagttc tgcacgattt gacgcagtga aagaacttaa tgattctgga attttttgtg
     3841 gcatattatt gactcctatg cttccatttt tgactgataa taaagatgaa atacgagcaa
     3901 ttgtagaaaa agcacacaag gctaatgcaa agtttattta ttgcatgtat ggtgtaacaa
     3961 tgcgtagtgg tcagagagag tttttttatg agcatttaag agatatatca ccaaaattag
     4021 tacttaaata tcagaaaaca tatggtttaa actatgtatg tactatacaa aataaagatt
     4081 cttgtgagaa gttgttaaga gaagaatgtg cacgttatgg tattttgaca gatatgaaag
     4141 agattataaa agcgtacaag aggtcggagt catatattca aataaaattt ttttaagata
     4201 ggctttggat gagatataat agtggtgatt gatgtgttga tattatagtt gattgttaga
     4261 gaggatatta tagatgaata ttaatattga tattctaggt aattgctaga actaatatta
     4321 tagatgaata ttagtattga tattctaggt aattgctaga gttagtatta tagatgaata
     4381 ttagtattga tattctaggt aactgctaga gctaatatta tagatgaata ttagtattga
     4441 tattctaggt aattgctaga gctgatatta tagatgaata ttagtattga tattctaggt
     4501 aattgctaga gctgatatta taatttatta ttaatattaa tattctaggg aattatcact
     4561 atattttaaa aagtaaaaat atatatcaaa atgaactaaa taagtactgt aagaagtttg
     4621 ttaaattaag gtttgaataa agtgagggtg gagaacttat gatagaaata aaaactatag
     4681 aaataaataa aaaaagtcaa attgaccttt taatgttagc cgacccatgt gaagaaatga
     4741 ttgataaata tctcaataaa ggaactatgt acgcattgta tgataataaa gaacttactt
     4801 gtatagcagt tgtaaatgaa atttccaaag aaatatgtga attaaaaaac attgctacat
     4861 atgagcattt tcaaaatatg ggttatgcct caaagatgat ttatcatctt ttagatgttt
     4921 atagtaaaaa atattcatca atggttgttg gtacatctga aagcggagtt cctttttatg
     4981 agaagtttgg atttgtttat tcacataaaa ttaaaaactt ttttgtagat aattatccag
     5041 aaccaatctt tgaaggagaa ttacaatgtg ttgacatgtt gtatctatca tataactttt
     5101 agataggata atatttttag gtagatataa ttaattaatt catataatta tcggaattat
     5161 aatttataaa ttatgtataa taagagtata atttatttag aagctgttat attgtattat
     5221 attcaaataa gatatataat aatttgttta ttttgaattt tggaggatta attatggatt
     5281 caaataataa tactataaaa tcaactgtta aaaagggtat ttcttttggt tcttgtttag
     5341 caatgattat ttcttatact gcatggaaat ctattccatg ggctattttt catggcttaa
     5401 tgagttggat atatgtactt tattattggg ttaagtatgc atagattaaa ttaataaata
     5461 agtatgtaat attgtaaagt ttaagaaaca aaatgtaagg gagtgtctcg aagtgaactt
     5521 tttagtttat aaggcactct tttttgtatt aaataaaata tattttcatt acttaaacaa
     5581 tgaaaaagat gagtatctct attttaatac atacccttat attcattcat ttatcaacaa
     5641 tagttttaaa aagaaagcta tctaaaatct attgtaatct aagtgcttct ctttaaagtc
     5701 ttaactttag taatgatata taagtttata aggttcttat ttcaataatg caaattttac
     5761 ttagaagttc agactttggt atgaagaaat gggtaataat aaaaaagagg tgttgatatg
     5821 aaagaaaaat tagaagaaaa atctaagatt attaaaacat caaaagagta taaacgaact
     5881 ataattgaag aaaaggacag agaatgaacc aaaaaattaa atgacatact tagtagaggt
     5941 gatttttcta atgaaaaatt agataaaatc aaggatttaa ttccaaaaga aatattgact
     6001 agtgaaaatg ttggagaagt tgtaactgaa gatgggtatg ctaagcctga gtatgatgag
     6061 gtatttaaat cattgtttac tttaaataat gattacgatt tacttgcaaa ttttataaat
     6121 gatatcctca aagatgctcc atatgccaat aggaatatca aaccgttcac tcatataaaa
     6181 agaataatta aggctgaaac cgacccaaca attaattaca ttggtgagaa aagacctaga
     6241 ctttatattt tggctgaaga tgaagaaagt aatcatataa atattgagat gcaaagagct
     6301 cttgaagatg attatttgga aagagctgaa tattatctta gtcgtgtaca tggtagaaag
     6361 ttagaagaag gtaaagagta taaagaaatt ggaaaaactg ttggaataca tatactaaat
     6421 catgtaaaat ataatcacat agaagattat gtaaattgcc tgagattaac aatggacggg
     6481 catccaaata tttattctag taaaactgca ttatatttta tagaacttcc aaaaattcgt
     6541 aaatccagtt gtatagcaaa cagagttcta atatggggta aacttattga taatccttct
     6601 catattgata ttagaatact atctaaaaca gattatgtta ttaaaagagc acttgataga
     6661 ttaaaagaat taggcagtaa tgaggactat ttattaaatt taaagagagg tgcgtatatt
     6721 atgaataaaa gtagaaattt taaagaagaa atttttcaag aaggtgttga aaaagggaaa
     6781 agagaagaga aatttaatat catcataaaa ttaaaaaata aaggatataa tttgagtgaa
     6841 atatgtgata ttatagatga tttgaataaa tcagaggttg aaaaaatcta taatcaaaac
     6901 taatactata attgagatgg cactagataa atataaagct tcctctttta aatttaaatt
     6961 aatataaaac cttgttattt aactatagta gatacaaaga gatatataag ctaaggagat
     7021 gattcaaaat aaaagtttga actatctcct tcttatagaa aaacaaaggg gttgatttat
     7081 atatgaaatt ttatactata cagactttag aattttggaa caacaataaa aacaataaat
     7141 atctatcaaa tgattattct tttatattag aagactttga tattccatac aaatggatgt
     7201 atagtcaaat gacaaataga atatatgaat ttgatgatag tatgatatgg gtttggacga
     7261 agagacctga tttaagatgt agtggttatt tagaaaaagg aaaaaaaggt gttttacttg
     7321 aaatagaaat aagtgaagac aatgtgttat tatcagaatt tgatttatgg catttacctt
     7381 taatggatat tactgtagag ctttatgaag atgaacaaat tgaaaagaag gtaagttggg
     7441 aaagaatatt tgacttagaa ctgtgttcaa aattaaatga agttaatata gaagatttga
     7501 cattgcaagg agtgacatct aaagtgcctc tatctaatgt aaaattaata aaagaatttg
     7561 ttgcaaaata atatttttat tgaaatttat tcaaaaaagt tatatattta taacaagagg
     7621 tgttataaat acataacttt ttaaggggga ttttatatgt ctaaacaaca aactggtatt
     7681 aaatcaacac ttatttcaga aatattggga atttcttatt gtattggagc tacattagta
     7741 aataagtatt tttttaaagg aagtagtatt gaatatcttc ctataattat ttgggtatta
     7801 gctatgggag ctcagtgggt ctataataaa aagtatgatg atttaattga tgaggcatca
     7861 aaatgtatac tatcaagagt taatgatatt gcaataaaag tattgttttt ttctgtagct
     7921 atagtatcta tatttcttgt aactttggac ataaatgtgt caaatttaaa tataggaatg
     7981 tctttattag tcattttgtt tattcaatcc ttgttgaagt taattctatt tatatatttt
     8041 gacagaaagg ggatatacaa ttaggtgtca gtattactaa agacaaaaat taaatcctat
     8101 agagaaaaga atcttatgac acaaggtgaa cttgcaaagt cagtaggagt aagacgtgaa
     8161 actattgtac atttagaaaa tggtcgctac aatccttctt taaaactggc aatggatatt
     8221 gctaaggtat ttgatacaac tgtagaagag ttgtttgaat ttattgaaga aaataatatt
     8281 taaatttcat gtattactta tgcattagag aatgaattaa tagataaaaa attcaagttt
     8341 atgtattaga gaatgaatta attataggta aaaaaccaag cttatagatt agagaataaa
     8401 tgacttatag atgaaaaacc aagctcatag attaaagaat gaatgattta tagatgaaaa
     8461 agaaagctta tagattagag aatgaattaa ttataggtaa aaaacaagct aatagattaa
     8521 aaaatgaatg atttatagat taagaagtaa gcttatagat tagagaataa atgacttata
     8581 aataaaaaga aagcttatag attagagaat gaattaatta taggtaaaaa acaagcttat
     8641 agattagaga ataaatgact tataaataaa aaccaagctt atagattaga gaatgaatta
     8701 attataaata aaaaccaagc ttatagataa gaagtccatg cttataagcc agaaaatgaa
     8761 tactttaata gattaaaaaa taca
//
LOCUS       DAGQWR010000160         8772 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.161, whole genome shotgun sequence.
ACCESSION   DAGQWR010000160 DAGQWR010000000
VERSION     DAGQWR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.161"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     regulatory      237..347
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            451..822
                     /locus_tag="MIW95_003328"
     CDS             451..822
                     /locus_tag="MIW95_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBY3152628.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQDNKKTPKWTENVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            918..1865
                     /gene="trxB"
                     /locus_tag="MIW95_003329"
     CDS             918..1865
                     /gene="trxB"
                     /locus_tag="MIW95_003329"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBY3152629.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            1950..2267
                     /locus_tag="MIW95_003330"
     CDS             1950..2267
                     /locus_tag="MIW95_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBY3152630.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            2536..3822
                     /locus_tag="MIW95_003331"
     CDS             2536..3822
                     /locus_tag="MIW95_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBY3152631.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            3934..4407
                     /locus_tag="MIW95_003332"
     CDS             3934..4407
                     /locus_tag="MIW95_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4066..4068,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBY3152632.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            4439..5749
                     /gene="grdB"
                     /locus_tag="MIW95_003333"
     CDS             4439..5749
                     /gene="grdB"
                     /locus_tag="MIW95_003333"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5486..5488,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBY3152633.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            6006..7538
                     /locus_tag="MIW95_003334"
     CDS             6006..7538
                     /locus_tag="MIW95_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBY3152634.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNSDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            7549..8694
                     /locus_tag="MIW95_003335"
     CDS             7549..8694
                     /locus_tag="MIW95_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBY3152635.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
BASE COUNT         3453 a         1006 c         1791 g         2522 t
ORIGIN      
        1 tgataaaata aactaaaaaa gatttgtact taaaaaaaaa agaaaataca agaaatatgc
       61 aatatcaaca tttggaaaaa aaagtaaaaa tgtatacaaa atacatataa aaaaactatt
      121 gcaaatttta tagaatgttt atatacttaa gtcaacagag atagaaaaaa gctatttata
      181 gaaaacaaag gataaattgc ttaattttaa attttctgaa aaaaataata atccggatga
      241 agagtatggg agactgcttt ataaaaagcg accgaaggga caagtttaat cttacttacc
      301 caaaagatta gatgaaattc tcaggtaaag gtgactgtac ttggacgggg ctctgaaaat
      361 gattttaaca gggaagacaa attgggagat ttgtttttcc tgttttttat ttatcaagta
      421 ataaataaaa gtttgaaaga gagtaaaaaa atgataataa ttacgaataa tccaaaagta
      481 aaagaagaag ttcaaggcag agaagttttg tttaaagaca caacttatat tggaatctta
      541 gaagcaagta gagatttgat acatgaaggt tatgaacttt tatctcatcc actctatggg
      601 agtgtaaaac caaatgaaac accttacagg actgttatac taaagaaagg aaatcgtttg
      661 gatataaact ctttaacctt aattgaagaa gcaatcatca ctgcaagtaa atttcaggat
      721 aataaaaaga ctccaaaatg gacagaaaat gtccaagacg attttagagt aattgattat
      781 gatatatttt ataatacaat acaaagaatg cagtatgaat aaaaaaattt gttaaaaagt
      841 tattgaaaca gcctaaatat taaatctaat ttaatattaa ataaaatgag aaatttaatt
      901 ggggaggata agtcaaaatg gaaaatgtgt atgacttagt aataattggt tcaggaccag
      961 ctgggcttgc agcaggtctt tatggagcaa gagcaaagtt aaaaactcta atcctagaaa
     1021 aagacaagac tggtggacaa attgtaataa cacatgaaat tgcaaattat ccagggtcag
     1081 ttccaaatgc aacaggacca agtttaatag ctagaatggt tgaacagtgt aaagagtttg
     1141 gagcagaaat gctaagagat aacattgttg atactgagct agatggagat ataaaagttt
     1201 taaaagggga aaaggcagaa tatagagcaa aagcagtaat aataggaaca ggagcaacac
     1261 caagaaagat tggttgtcct ggagaaaaag aacttactgg taaaggggtt tcatattgtg
     1321 caacttgtga tgcagatttc tttgaagatt ttgaagtatt tgttgtaggt ggaggagaca
     1381 gtgcattaga agaagcaatg tatttaacta aatttgctag aaaagtaaca attgtacata
     1441 gaagacaagg atttagatgt gctaagagtg ttgaagaaaa agcaaaagct aatccaaaga
     1501 tagagttttt attagataca gtaattgaag aaatcaaagg tgatggaata ttagaatcag
     1561 ttgtatttaa aaataaagtt actggagaaa ctcatgaata ctttgcagat gaagaagatg
     1621 gaacaatggg agtatttgtg ttcgttggat tagatgctca aactgattta ttcaaaggta
     1681 aagttgatat ggatgaaaaa ggttacataa taactgatga agatatgaga actaacatac
     1741 caggcgtatt tgcagcagga gattgtagat ctaagactct aagacaggtt gtaactgcaa
     1801 ctaatgatgg cgcaattgca tctatagtag ctgagaaata cattgatgaa aaatttggga
     1861 actagaatct gtaaattaag aaatagtgtc tgtaaattta atgaataata tttaaaatta
     1921 aaataaaaat ttcaaggggg aataagaaaa tgttagattt agataaggct acatttgaag
     1981 aagaagtttt aaatgcagaa ggttttgtat ttgttgattt ttggagtgaa ggttgtgagc
     2041 cttgtaaagc tttaatgcca gatgttcata aattagctga gacttatggg gataaaataa
     2101 aattctgtaa gatggataca acaaaagcta gaagattagc tataaaacaa aaagtattag
     2161 gtcttccaac tatggctata tacaaagatg gagaaaaagt agacgaagtt actaaggatg
     2221 atgctacagt tcctaatata gaaaacatga ttaaaaaata tctttaatat gttgataaag
     2281 tattaacatt aaaagaaaaa atctattttt atctgaagat aagaagaaaa aggtttttat
     2341 gtccaacttt gttaaaaaaa taattttaaa taaaataatt tttttacaat aaatattttt
     2401 tagtaataaa ataaagattt aaggcataaa aacacaataa tatatcttta aaaaaataag
     2461 ataaattgta aaatgatata attttgagaa aaactcaaat gcatatacac aaattaaata
     2521 acaggaggtg caagtatgcg tcttgaatta gggaaaatct ttataaaaga tattcaattt
     2581 ggtgatgtaa cagaagtaaa agatggagta ttattcatca ataagcaaga gatgctacaa
     2641 gaaattggtg gagatgagca tattaaatct atagatatag aattagctcg tccaggtgaa
     2701 agcgtaagaa taacacctgt aaaagatgtt attgagccaa gagtaaaagt tgaaggtaat
     2761 gggggaatct tccctggtat catgtctaaa gttgatactg taggagaagg aaaaactcat
     2821 gctttaaaag gtgtagctgt tgtaactact ggaaaaatag taggtttcca agaaggtata
     2881 atagacatga ctggagaagg agctaagtat actccatttt ctaaattaaa taatgtagta
     2941 gtaatagctg aacctattga tggtttaaaa caatacgctc atgaaaaagc tgttagaatg
     3001 ataggattca aagcagcaat gtacttagga gaagtagcaa gaaatcttac tccagatgaa
     3061 gtttcagtat atgaaactaa acctcttctt gaatcaataa aagaatatcc agagcttcca
     3121 aaagtaggat atgtttacat gcttcagact caaggtttat tacatgatac ttatgtatat
     3181 ggagtagatg caaaacaaat agtaccaact ttattatatc caacagaatt aatggacgga
     3241 gcaattgtaa gtggtaactg tgtttctgca tgtgacaaaa acccaagtta tgttcatata
     3301 aacaatgggg ttgttgaaga cttatatgca cgacatggta aagatataaa ctttgtaggt
     3361 gtaattataa ctaatgaaaa tgtttaccta gcagataaag aaagatcttc aaactggact
     3421 tctaaattat gtaaatattt aggattagat gcagttatag tttcacaaga aggttttgga
     3481 aatcctgata ctgaccttat aatgaactgt aagaagatag aaatgcaagg tgttaaaaca
     3541 gttatagtta cagatgaata tgctggacgt gatggaggtt ctcaatcact tgcagatgca
     3601 gatgtaagag ctgatgctgt tgtaacaggt ggaaatgcca accaagttgt agttcttcct
     3661 aagttagata aagttattgg acatttagaa gtagtagatg taatagctgg tggttctgat
     3721 ggaagtttaa gagctgatgg aactattgaa gttgaaatcc aagctataac aggtgcaact
     3781 aatgaaacag gatttggtca tttaactgct aaaggatatt aagaagtatt ttattaaacg
     3841 attgagtaat gttaataaaa catattatag ataattataa taagtgttat agatatataa
     3901 aaatatatta gaaatataat aaggaggatt attatgagtt tacttagtaa taaaaaggtt
     3961 cttataatag gtgaccgtga tggtatacca ggacctgcga tagaagaatg tgtaaaaaca
     4021 gtagaaggag cagaggttgt tttctcatct acagaatgct ttgtctgaac agctgctggg
     4081 gctatggact tagaaaatca aaacagagtt aaagatgctg ctgataaatt cggagctgaa
     4141 aatgttgtga ttttactagg tgctgctgaa gccgaagctg caggtcttgc agccgaaaca
     4201 gtaactgctg gagatccaac tttcgctgga ccacttgctg gagttgcctt aggattaagt
     4261 gtttaccacg ttgttgagga accaataaaa tcattatttg atgaaagtgt atatgaagac
     4321 caaataagta tgatggaaat ggttttagaa gttgaagaaa tagaagaaga aatgtctggt
     4381 ataagagaag aattttgtaa attttaatgc ctaaaagcaa aaggggtgaa actaagagat
     4441 gggaaaacta aaagcagtac attatattaa tcagttcttt gctggtatag gtggagaaga
     4501 aaaagctgat actaagccac atgtggcaga aactttacct ccaataagtt tacaacttga
     4561 taaattatta ggtgaagata ttgaaatagt tggaacagta gtttgtggag atagctattt
     4621 caatgaaaat attgatagtg caagtgaaga agttttatca atggtaaaag gttttgaacc
     4681 acaacttttc atagctggtc ctgcatttaa tgctggtaga tatggggttg ctgctggaac
     4741 aataactaaa gttgtaaaag atgctttgaa tataccagct ttaacaggta tgtatgttga
     4801 aaatccagga gcagatatgt tcaaaaagga tgtttatgta gtagaaactt ctgattctgc
     4861 agcaggtatg agaaaggcac ttcctaagat agctaaactt gcagtaaaac ttgctaatgg
     4921 agaagaaatt ggaactccta aagatgaagg atacatagct agaggaataa gagttaacta
     4981 cttccatgaa gacagaggtt ctaagagagc tgttgacatg ttagttaaga aaataaaagg
     5041 tgaaccattt gaaactgaat acccaatgcc aaactttgat agagttgacc caagtaaagc
     5101 tgtaaaagat ttatcaaaat gtaaaatagc tttagttact tctggtggta tagttccaaa
     5161 aggaaatcca gatagaatag agtcttcttc agcttctaaa tatggaactt attctatagc
     5221 aggagttatg gatttaacag aagagactta tgaaactgca catggtggat atgacccagt
     5281 gtatgcaaat ttagatgcag atagagtatt acctgttgat gttcttagag atttagaaaa
     5341 agaaggtgta attggaaaat tacatgagac tttctataca actgtaggta atggtacatc
     5401 tgttgcaaac tctaaaaaat atgcttctga aattggagca gcattagtgg ctgatggtgt
     5461 agatgcagta atattaactt ctacatgagg tacttgtact cgttgcggtg caacgatggt
     5521 aaaagaaata gaaaaaactg gacttccagt agttcatatg tgtacagtag tacctatttc
     5581 attaacagta ggggctaata gaatagttcc aactatagca atacctcatc cacttggaaa
     5641 tccagcgctt gaccctacag aagaaaaagc cctaagaaga ggccttgtag aaaaagcatt
     5701 aaatgcttta actacagaag tagatggtca aacagtattt gaaaaataaa aataacacac
     5761 acatatatta tatagataaa aatataaaca cacactaaaa ataaaaaata actatacaca
     5821 aaaaaaataa tatagatttc atgaatggat aaattggagt tattcattca tgaaatctta
     5881 aaaaatgcca aaatacataa acaataatag aatcaaatat acaataaaat acaatgatta
     5941 gagcttttaa taagtttaat aagattaaaa aataagtgtt cataaaattt atggaggtgc
     6001 acaaaatgac ttatccagtt ttaaaagggg caggctatgt acttattcat actccagata
     6061 tgatagtgca aaatggaagt acttgtacag ttgaaagagc aacaaattca gattcagaat
     6121 ttttaaaaga agtaagtaat catataagaa gttatgaaga tgtagttaac tacatgccaa
     6181 atcaagttta tataggaaat agaagaccag aagaattaag agatttacca atgccatggt
     6241 gtgaacaaaa gattgaggga actagaaatg gtaagtttgg agaaataatg cctcaagatg
     6301 agttcatagc tttaatgcaa ataagtgatg catttgattt agtaaaatta tcacaagaat
     6361 ttattgatga agtaaagcca aaaatcgaaa ataactaccc tgaaattgca ccatttgtag
     6421 gtaaattaaa gggtgatgat atagaagaag gtaaagaatt agtagcaaca cacatagctg
     6481 aaggattata ccatgatgga aaatttgttg gttatgtaaa aagagcacat gatgtagatg
     6541 ttaacttaaa tgcccataca atgtttgaaa atctagtagt aaaagcatct ggagttttat
     6601 ctgctattca aatgctaaga catagcaaaa tagaccctgc tgaaatagat tatgttattg
     6661 agtgttcaga agaagcttgt ggagatataa accaaagagg tggaggaaac tttgctaaat
     6721 caatagctga gatagcagga cttcagaatg caactggttc tgacacaaga ggattttgtg
     6781 cagcaccaac tcatgcatta attcaagcag cagctttagt aaaagctggc atacataaaa
     6841 atgtaatggt agtagcaggt ggagcaagtg ctaaacttgg aatgaatgct aaagaccatg
     6901 ttaaaaaagg tcttcctgta cttgaagatg ttgttggagg atttgcagta cttgtatctg
     6961 aaaatgatgg tgtaaatcca gttataagaa ctgatttaac aggtaagcat actgttggaa
     7021 ctggttcttc accacaagca gttatgacag cacttataac ttcaggtctt gatagagcta
     7081 atttaaaaat aacagatgtt gatgtgtatt cagttgaaat gcaaaaccca gatataacta
     7141 agccagcagg agcaggagat gttccagaag caaactacaa aatgataggg gcacttgctg
     7201 ttaaacgtgg agacttagaa aagaaagaat tgaaagattt tgtttctaac aaaggattac
     7261 caggatgggc accaactcaa ggacatatac catcaggagc tccttatata ggatttttaa
     7321 tagatgattt aactacagga aatagaaata gagctatgat agttggaaaa ggtagtttat
     7381 tcttaggaag aatgactaac ttatttgatg gagtatcttt tatagctgaa agaaatacag
     7441 gtgttactga agagacttca ggaatatcta aggatgaaat taagaagata atagctgaat
     7501 ctatgaaaaa actagcatta gatatgttag aagaataggg gtgaggatat gtcaaaaaaa
     7561 gtaatcgcag atgtattctt agaagtagct aatgcaatag aaagcggcga atttggcaaa
     7621 aaagtaaaaa ttggtgttac tacacttgga agtgaacatg gtgtagaaaa catggttaat
     7681 ggtgcacaat tagcaaaatc taatttattt gatattgtgc ttattggacc aaaagtggaa
     7741 actgaccttg aagtagttga agttaatgat gaaaaagaaa tgcatgcaaa aatggaagaa
     7801 ttattagatt caggatatat tgatgcttgt gttactatgc attataattt tccaataggt
     7861 gtatctacag taggtagagt aataactcca gcaaaaggaa aagaaatgat acttgcaaca
     7921 acaacaggaa caagtgctac taatagaata gaggcaatgg taagaaatgc catttatggt
     7981 attgctacag ctaaatcaat gggtaacaaa tgcccaaaag ttggaatact taatgttgat
     8041 ggagcaagac aagttgagaa gtgcttaaaa gaattaaaag ataatggata tgacatggag
     8101 tttgcggatt caattagagc agatggtgga tgtgtaatga gaggtaatga tttacttgta
     8161 ggtgcacctg atgttatggt tactgataca ttatctggaa atatatttat gaaagtattt
     8221 tcttcatata ctacaggtgg agattatgaa gctcaaggct ttggatacgg acctggagtt
     8281 ggagaagact atgatagaaa ggtacttata gtatcaagag catcaggttc tccggtagta
     8341 gcaaatgctt taaaatacgc ttatgatgtt gtaaagggag atataagtaa tgttgcaaga
     8401 aatgaatttg caaaggttaa aaaagcaaaa tttgatgaca ttatatcctc tcttactaaa
     8461 aaagaagtta aagctgaaaa agtagaagtt aaaatgccag ataaagaaat tgttactcgt
     8521 caaatagctg gtgttgatat aatggattta gaagatgcag tatctgagtt atggaaaaac
     8581 ggaatctatg ctgaaagtgg tatgggttgt acaggtccaa tagttttagt aaatgaagct
     8641 aaaggagact tagcagttga aactttagtt aaagctggat atactgcaaa ataagtacat
     8701 tttttatatg taaataagaa catatagtta taattttatt gattataatt atatgttctt
     8761 tttattttat at
//
LOCUS       DAGQWR010000161         8641 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.162, whole genome shotgun sequence.
ACCESSION   DAGQWR010000161 DAGQWR010000000
VERSION     DAGQWR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.162"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            576..2810
                     /locus_tag="MIW95_003336"
     CDS             576..2810
                     /locus_tag="MIW95_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY3152636.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESSRELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLILFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2841..3686
                     /locus_tag="MIW95_003337"
     CDS             2841..3686
                     /locus_tag="MIW95_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY3152637.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3926..5659
                     /locus_tag="MIW95_003338"
     CDS             3926..5659
                     /locus_tag="MIW95_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY3152638.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDVFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   6051..6737
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6051..6079
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(7268..7660)
                     /locus_tag="MIW95_003339"
                     /pseudo
     CDS             complement(7268..7660)
                     /locus_tag="MIW95_003339"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            7720..8213
                     /locus_tag="MIW95_003340"
                     /pseudo
     CDS             7720..8213
                     /locus_tag="MIW95_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         3463 a         1083 c         1093 g         3002 t
ORIGIN      
        1 aatatatttt agttttatat agttttatgt ttacagctat tatatccatc tctatgtaat
       61 atttttcata ttaaaactga tttctataga ttaatagttt ataaatttag taaatgaata
      121 tttattatag tctttaacaa aaaatacaag aataaactta attttaataa ctaaaattaa
      181 ttatgtggta ttaaattatg tataaaaagt taattatgaa taaaataatt atgcctttga
      241 aaaatcatta aacttaaaat taattataaa aaaactctac cttactaact atacagctca
      301 caatctatct atttatgaaa aaatatgata taattgcatt caaaaaggaa atctttcaat
      361 atactataat ttaagcatta actaatttta tctaatatta ttactaaact aaaaaatatt
      421 tgtatttgta gcattggagc aaataactta atgtatattc attttaaata cttatttaaa
      481 atttagcatt taaattattt acaaattaac atttaaatac ttctagcata aatacagttg
      541 tcatcaaaat ttaaagtatc ttatggagga ataccatgca aaatcataat tatataaaaa
      601 tgttaaataa aaaggtagaa gtattatcta aggaattaga tagtagaaat tttaaattaa
      661 aaaccttaaa tgaaatagat tttttttcta aaataccaaa cagagaattt atgtaccaat
      721 atattgatga ctgtatttcg aaaaatatac atataggaat catgttatta aatatcgata
      781 actttaaatt atttaatgat atatatggat atctagtcgg agaaaattta ttaattactc
      841 taactcaaaa cctattgcac tttattccag aatcaaaggg aatagtcgct tatttaaaag
      901 gagaaggttt tttaattgta cttccaaatg tatcaaataa agagttaaat aatatgggtg
      961 caactattat agagagctcc cgagaattaa aaataagact tcctaatgaa aaagaaatat
     1021 actccaatgt tacatttagt atcggcagca ctatctggaa tggtgaacca aacatgacta
     1081 ctttgatact tttcaaccaa gtatatagtg ctttaaaaaa ggctaaaagt gaaggcaaaa
     1141 atagatacat tctatttgat tttaaagata atagttttta caataaatta gaaaatgata
     1201 aggagttctt atcagaaata tcacatgttc tacaaactaa acaaattgaa gtattttacc
     1261 aaccacttta taatataaaa aataatacta tagttggatg tgaggcactt gcaagatgga
     1321 gacaccctat taaaggactt cttactccag ataaatttat tcctttattt gaacagtttg
     1381 gtctgataac ggaattagat ttgcatttat ttgaagagaa ctgtaaaaat ataagaagat
     1441 ggattgataa caatgatgtt tttgttccaa tttgttgtaa cttttctcgt cttcattttt
     1501 ttaaccctaa ttttccaagt atgctaaaag gtattactga aaagtacagc gtctctacat
     1561 caaattttag cattgaaata acagaaaata tattaataga aaatacaaat acaattattc
     1621 aacaactagc tgaacttcgt tcttatggtt tttctgtatc aatggacgat tttggttctg
     1681 gatattcatc ttttggtatg ttgcaaaatc taccttttga tataatcaaa attgatagga
     1741 tatttttatc tcataattta aatgatttta aaaatacaac cattttgtac tctataataa
     1801 acattgcaag agctcttggg atgcttacag tatgtgaagg tgttgaaaca gaacaacatg
     1861 tagaatttat taaaagtata aattgtgata tcgcccaagg ttattattat gcaaaaccca
     1921 tggagtgcaa agaatttgaa aaattactat ctaaagagaa taattcagag aaaatgttta
     1981 taaaatattc tgatatagga aaatctttaa tagagaagat atttgatgga ttttttatta
     2041 tgcaagatat gaaaaaacta agagctaatg tcactgaaga cattcaatgg tttgattttt
     2101 acaacaaaac tgaaattaca tcttttccta aagtaataga acattttgaa aaacatatta
     2161 aaggtaaaaa attttctatt ctttacaaaa gtataacccc acataatact gaatcagatt
     2221 cattattaat tagtggagaa gccgttttat cagatgaagc ttctgaacat caaaaatata
     2281 gcaatttcta ctttagtgca aattgtataa tttcagaaaa taaattactc ttagcaaaat
     2341 tacatttgga tttaattaaa actgctggct atataaatga atttgaatca tattcaatac
     2401 ctaaaatgac aaatacacat cttgatgaaa gtaatatttt atcacaattt tattcaatgt
     2461 tgcctgtagg tataattcgc tacgatttat ttggtgatat gattattact tatatgaatg
     2521 aagaaatgtt tgacattata ggttatacaa aagaacagtt ttttggtgag atgggcggaa
     2581 atttaagagc aattgttcat ccagatgatt tagactttgt ttataaaaaa agtttggata
     2641 tgattgacac aaataaaatt gaaccatttt tatatagatt cataaaacga gatggtcaaa
     2701 ttgtcacagt tatgtataca caatgtaacc tttctgcaat tgatggaagg tcgattgccc
     2761 aaggtatgta tgtagatgta aataaaataa aaaatttaac aaaaatctag tacaaagtat
     2821 tttattttgg agaaattaat atgtttaaag aaattttttt aagaactttt ccaggatttc
     2881 taattataag agattctaac tacagaatta tatttataaa tgataattta aaaaatttaa
     2941 ttaaaagtta tatgcaagat aatccacttg gaatgactaa tatagaaata gctaagaaac
     3001 tccctgataa tattgctaaa ttttttacgg attctcacaa tattcaatta gattgggaaa
     3061 aaaattatcc ttatgataaa atttcaaatt ggattttaga gtttaaaaaa gatactacaa
     3121 gctattggaa tgttttagaa tacaaagtag atgttgatga aaaaacctac ataattacta
     3181 tggccaatga tataacaaaa ctctatgaag aaaataagag aaatcttcac tactcaatta
     3241 ctgacccatt aactggagca tataatagaa aatatttaaa tgatagattt gacatattta
     3301 taggagatta tattgtacta attgacttag ataatttcaa aatgataaat gactatgaag
     3361 gtcataatgt aggagacaag attttatgtg attttgtttc acttttaaat aaagaattaa
     3421 taaattctac ttctattata agattaggag gagatgaatt tattgttata ttctctagtg
     3481 atgttgacaa aaattatgtt tattcacaac tagaagcctt aagagaaaat tttttaaaag
     3541 tattctctaa atataagtat ctttcattta gttatggtgt tgatacagta aaaagaaatt
     3601 taaaattgac aatagttgaa ttggataaaa aaatgtataa aaacaaggag aaaaacaaga
     3661 aaaaatttga taaaaatgat tattaacatt gttattttaa aataaattaa aattatcttt
     3721 ctttttttaa acttgtacat tagtttataa atatgtatct atacaagata ttgacattaa
     3781 gtactgaagc atatattgtt tcagttcttt tttgttaaaa ttaacttatt tgacaaagat
     3841 atcatagttt aacaaagctt ttaatttaaa tatattgatg tataatataa ttaaaatata
     3901 atagcaaaaa gaaaggagga atatcatgag catactccta aaaaaagcac ccaaattagc
     3961 aaaacatatt ataacaagtt tttatataaa tcgagatatc aatgaagtat taaaatacct
     4021 ttgtgaaaat gttacatgga ttggaccagg agaacaggaa tttctcactt cttttaatga
     4081 aataaaaaat tatttttatg caggtcaaga tgaaattcct tcttgtgata taaataatga
     4141 tgtatttgaa actgtaagtg aagatgagaa tagatgtatg gtattaggaa gatatactgt
     4201 tcgaactaaa gaaaatgctc aaatgatttt agaggtaaat caacgttgta catttgagat
     4261 aattgaggat agaggaaaat tattagtaaa gcacatgcac atatcaaacc cttatggtga
     4321 aatgcaactt gatgaatact ttcccactaa aattggaact caaagttatg attatttgca
     4381 aagattacta aaggaaaaaa cagaagttat tgaaatgatt acaaataata tcaatggtgg
     4441 cttaaaaggt agcaatgatg atagtacata ctcatttttc tatgtcaatg agggattgcc
     4501 caaaatatta ggatacacat atgatgagtt catggaaatg agtggtggtt cagcagtggg
     4561 tgctgtatat ccaccagact tacctaaagc actagaagat tgtgaacgat gttttgcaaa
     4621 aggacctaca tattcatctg aatatagaat ccgaaaaaaa gatggaacat taatgtgggt
     4681 attagattct ggaatgaaat cgttaaatag tgatggcata gtaaaaatta atagtattat
     4741 tactgacata acccaactaa aaaacataga atctgaacta aaattagaaa gagagcgata
     4801 tcgtattgct ttgcaaaata ttacagatat catgtttgag tatgatattg aaaacgatac
     4861 ccttataaaa tatcaacgtg tagaaattga caaaaaaatt gaattagaaa attttgaaac
     4921 aaaaaattac tccaaactat tagaatctgg taaaattatt caccttgatg atattggaaa
     4981 actactagaa gtacttcatg gaaatcttcg tgaaacaatt gagataagac aagtaaattc
     5041 attaactaaa aatgaatgga gatggattcg tgttcaatgt tcagttatat atgatagtga
     5101 ccataaccct atcaaaacaa taggcgtttt aaaagacata actgaagata aatctaaatt
     5161 agaaagctta attaatcagg ctcaacgtga cccactaaca caattatata atcaaagagt
     5221 atcacaaaat cttattgaag agtatctatg tagttctgat tccaaaaata atgatgctct
     5281 attagttatt gatattgatg attttaaaac tgtaaatgat acttttgggc atttagaggg
     5341 aaatgaagtt ttagttgctg tatcaaaaat actattacat aacacacatg ataaagacat
     5401 agttgctcga attggtggag atgaatttac aatatttata aaatcattaa caaaggattt
     5461 aataataaaa attactaatg atattttaaa tgatgcaagt aaaataaaag taaaggacaa
     5521 tcataaaata acattaagta ttggtattgc cttcactgat gattcaacta aattatataa
     5581 agatttattt tctaaagctg ataaagctct ttatctatca aaagcagatg gcaaaaattg
     5641 ttatagtaca tacgaatgat aaagctattt atacgaccaa gaatgaacct ttagatttaa
     5701 tttcatagtc taacaatcaa agagttgaag ctaatcttat agctagtcaa ctagttccta
     5761 gaaaaaattt agacaatata tttattgata ctgctcattt ttctaaagat caaatttata
     5821 taaaaaatta aacttttatg tacatacaga tactaatgat aaacatttag cattagtatc
     5881 ttctatctta aaataaaatg ataatttatc tatataaaaa agtgctttgt tgacagagtt
     5941 cactttttta tataggtaca gaatttaagt ataaatacat gttttattct atacatttta
     6001 gttaatgtaa aatttcttga ttgtactctc taacttttca acattatact atttacatac
     6061 cacttagtta atataaaacc aaaagttgga gaaatagaag tgccattcac attcgaattt
     6121 acataccact tagttaatat aaaacacttt aataattcta cctttactag tgctaaagta
     6181 atttacatac cacttagtta atataaaacc aggaacatat ggcaaactat aaggaaaacc
     6241 tgcaaattta cataccactt agttaatata aaaccctctg tgactccata ttttttcata
     6301 atatataatt catttacata ccacttagtt aatataaaac atagaagttg ttgcatataa
     6361 ctacattgac aagttattat ttacatacca cttagttaat ataaaacttt tctacttttt
     6421 taatcattta atcacctgct taaaaattta cataccactt agttaatata aaacttttga
     6481 tattctaaag aagtgacaat gatgtgacac aaatttacat accacttagt taatataaaa
     6541 ctattatgta gtcatatatt tcaaactcta atttcgtatt tacataccac ttagttaata
     6601 taaaacccat ttgacccatg catattgact tccaccttcc ttatttacat accacttagt
     6661 taatataaaa ctttacaata tagacgtaca tatacttctg aaaaacctct ttccatacca
     6721 cttagttaat ataaaacttt accccaaaat aaactgggca ttttcaatac ttacacatgt
     6781 gcaactctct taaatttgca gtgaaccatg agtagtgcaa atgataaagt ttatcatata
     6841 ccctcaacac cttatatttc aagcattaag ccctactttg taacaaatat cgctcactgc
     6901 aaaactattt acatttttat tatatcataa atatattatt tttgaatatc tgtaccaatt
     6961 tgtggtataa taaaaacaag aagaactaca atctatttag acgtagagtg aagttcaaat
     7021 actaatgttt attctttttg aacttgattt ttagttcaaa actaaagtca ctcctgctga
     7081 actataccca ctgttttata cagttaaaac atgtaaaagt agttataaag tactagtgta
     7141 actcattagt acttttttta ttatattcat ataattaata tctatatttt aaagtttact
     7201 atctaaaaaa gagcacgtca aataaaaccc tagatattta aattttttta gagctactta
     7261 aaataaatta tgctatctct gtacttaaat atttcctaaa ttcaagtgga gacatagaat
     7321 ttaatctctt ttgatatcta aaattattat agtaatatat ataagtctct attattttaa
     7381 ctaattgttc atatgtatca aatttcctta aatagtacat ttccaccttt aaaattcccc
     7441 aaattccttc cataggagca ttgtcaatac aacgaccagc tcttgacata ctttgtatca
     7501 taccttgtgc taaaagtttc gattcaaaca ccttactagt atactgaaaa cctctatcac
     7561 tatgaaaaac aggttttgca tttggatgta gttttattac tttatcaaaa gtttcgaata
     7621 ccaagttatt attattcgat tttccaataa catatgaaat taatagatat aacgccttct
     7681 attttacagt cttatataaa taacatatac aatgagcaca tgttaaattc aataaaaaac
     7741 caagtcgatt ttataaaatc agtattgaaa gaatcttata gattaaaaga aatatcagaa
     7801 aatatttgtg attttgttac aaccccaatc aaaaaaaata gttcaacttc taagttttat
     7861 acaaagcaag aaactcaatt attattagaa aaatcaaaaa atattcctat aggtatacca
     7921 atttttttga tactaactct tggattacgt tttggtgaag ctgtatcact tatatggtca
     7981 gatgtagacc ttgataaaaa atctcacatg taaatcaaac attagtttat gtatacaata
     8041 aaataatctt caaagaccct aaaatcccta gatccaaaag aaaattattt gctcctgatg
     8101 aacttataag tttgcttaaa gggcaaaaat taaaacaaaa taagttaaaa ttacagggta
     8161 tattgaaaaa tgaatttgat ttgatatgct taaataaaag attcaacccg tgagttcaac
     8221 caacttttgt tgttcatttt aaaaaactat taagagataa taatcctaga gatataaaag
     8281 tacatgaatt aatacatgca aatgcgactt tgatattact ttcaggtact aacataaaag
     8341 ctatttataa aatattagta catgcagata taaaaatagc tatgaataaa tattcgatat
     8401 tctcacttat tagatgaaat ggataaagaa acatctgaaa atataagcaa aatactattc
     8461 aaataatgtt cagttttatg tcaattaaat gtcactcaaa gtgctttttt tccatttgtc
     8521 agttaaatgt cacttaaagt acctttttcc tatttgtcag ttaaatgtca gtaatatata
     8581 aataagatat atttataaga cccttaaaat cactattatt taataaaatt tataattata
     8641 t
//
LOCUS       DAGQWR010000162         8602 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.163, whole genome shotgun sequence.
ACCESSION   DAGQWR010000162 DAGQWR010000000
VERSION     DAGQWR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.163"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            139..369
                     /locus_tag="MIW95_003341"
     CDS             139..369
                     /locus_tag="MIW95_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBY3152639.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            382..531
                     /locus_tag="MIW95_003342"
     CDS             382..531
                     /locus_tag="MIW95_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBY3152640.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            788..1225
                     /locus_tag="MIW95_003343"
     CDS             788..1225
                     /locus_tag="MIW95_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBY3152641.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            1482..2375
                     /locus_tag="MIW95_003344"
     CDS             1482..2375
                     /locus_tag="MIW95_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY3152642.1"
                     /translation="MQLYNTPFNTNKHLIYFFTSTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            2553..3377
                     /locus_tag="MIW95_003345"
     CDS             2553..3377
                     /locus_tag="MIW95_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY3152643.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKHEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREVVPDYQSEGILWEKLSKFIKEEHIE
                     VSRQPNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            3476..4084
                     /locus_tag="MIW95_003346"
     CDS             3476..4084
                     /locus_tag="MIW95_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY3152644.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            4188..4364
                     /locus_tag="MIW95_003347"
     CDS             4188..4364
                     /locus_tag="MIW95_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152645.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            5660..6412
                     /locus_tag="MIW95_003348"
     CDS             5660..6412
                     /locus_tag="MIW95_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152646.1"
                     /translation="MLKVNQLCKSYKTGNKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            6402..8513
                     /locus_tag="MIW95_003349"
     CDS             6402..8513
                     /locus_tag="MIW95_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152647.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYI
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVAGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIERVYMKKSSYDMFFNSK
                     DFSKLIDKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         3261 a         1104 c         1129 g         3108 t
ORIGIN      
        1 taatactaac aagtaaaaca ttaatattga caattgaaat attaatacta acaagcaaaa
       61 cactaatatt gacaattaaa ataccaatat taacaagcaa acaattaaaa tataaattat
      121 tatatcggag gtaattatat ggaaattaag aaaaaattag tcatgagctt taaaaatgta
      181 aaggataaac aagtttcttt ttctatagaa aatccaaagg atgatttaac tgaagaaaat
      241 ataaaatctg tcatggattt gatagtatct aaaaatattt tttcggttgg aggatttgac
      301 ctagcttctg ttgttgaagc caaggttgtg gaaacaaata ccactcctta tgacttagtt
      361 atagaatagg gggctaattt tatggatgat agtttacaac tgttaataac caatgttggt
      421 tttcctatag ctctatccat gtacttgttg gttagaattg agggtaaatt aaatacttta
      481 tcaaacagta ttgatacatt atccaaaaat attttgatat ttaagaagta atttgacaat
      541 atatgtaagt cataaaatag gtctatttgt aaaataatta gacatattct atgctaaata
      601 ctaggatatt tggataattt atccaaatat ccatagtatt tttttgaatt ttaccacctc
      661 aaatttgaat ttcactattt tatttttgaa tttcactatg cttaatttga aatttagaac
      721 gcatctagtt ggactcaata agatataatg ctattctaat gacagtaata aaatgaggtg
      781 atgattaatg aaaatacatg tcgaacaagg tattaattgt gatgatattg aaataaccat
      841 aaaatgttct gctctagata ataacattga aaaaataatt tcattattga gtacaccatt
      901 acttgatata aaaggtaaaa aatcaggaga gatttataaa ctcaatgtct gtgatatttt
      961 atactttgaa acagttggaa ataaaacatt tgcttattat gaagatgatg tatttgaaat
     1021 tgatttaaaa atatataaat tatcaaaatt attagctaat acatccttct cacgaatatc
     1081 taaatccatg attttaaata tggactacat aaaaagtata aaagtaatat cacataatcg
     1141 catgcaagct actttaaaaa acaacgaaaa agttattata aatcgtcgtt atataaaaaa
     1201 tattaaagaa aagctaaaca tttaataaaa atctaaagta atttattaat atcactaatt
     1261 gcaaatttat tgacctattt aataattaat aataatataa tatgttcaat attaaatact
     1321 aattaattat atttagtgta aaaataatta atcaaaatat aaaaaatagg atattattat
     1381 aggcaataac taacatcaaa cgataactta atacatataa aaataaataa acaattaata
     1441 aaaattaaaa cttcattata aaacggaagg atagtgaaaa aatgcaacta tataacaccc
     1501 cctttaatac aaacaaacac ttaatctatt tctttacatc tacgctagta ttgaactgga
     1561 tattcagtct tacacctgcg ttgctcggaa ttacaaatac aatacttgga gaaatcattt
     1621 tttatcttgc atatacatca ccagctattg taggtctatt ttttatttct acaatctatc
     1681 cacaaagtgc aaaacaagat tttatttata gatacctaag ctttaaagaa attagaataa
     1741 aatggatact attaacaata ttattttttg ctttaacttt tattacttcg ttaataatcg
     1801 gaaattactt taatgtaaaa acttctgata tagattggat acacatagca gtgtttagac
     1861 cttctaaaat agcttatatg ctattagcaa gttttatatt gagtatactt agtcaagaag
     1921 ctggatggag aggatacgcc attgacaaat tattagttag atttggattt ataggctctt
     1981 caattatttt agggcttgtt tgtggaatct ggtatctagg gtcgtatttt acacctaatc
     2041 aaataccata taatttggca caatactccc tatttgatgc ttttttattt ataccaaaca
     2101 ttgtactttt aaactttata attaattttg tatatatcaa tacaagtaga agcattttat
     2161 cagctgggtt agttcatatg atgtactatt ttttcaacat tcaattactt ctacactacc
     2221 ctattaaatt atgtgtaata gctcaatatg tacaaataat atttggttta atatttcttg
     2281 tatatatgat ttcttctaat aaatttaaac agaaggtaaa ttctgaaatt gaacagataa
     2341 agtcggatga ttttgaattt gagttagact ggtaatttta atatgaatta taaattaaga
     2401 taatctatga tttgatacga ttactactta aatataattt actctctata atttattgca
     2461 acatctggaa aataattgta tttccacaat cgcttgaagt atataaaaac aaaatatata
     2521 ctaattataa gtgaaaagga ggtcatattt tcatgtttaa aataggagag gtttcaaaac
     2581 taacacaaat ttctattcgt atgttgcgtt attatgatga acttggtatt ttaaagcctg
     2641 cagaaataga caaatataca ggtcatagat tatactcagt tgaacagatt tcaatattac
     2701 aaagaattgt attattgaga gattcaaaat ttagcgttgc agaaatagca aatatcgtac
     2761 ataattggaa tgatgaattt gtaattaaag aattgaatag aaaaaagaat gaaatacaaa
     2821 aggaaataaa gcacgaacaa caaagaataa ataaaattga taaatttata gaagctataa
     2881 actgtgataa agatgaaatt cattacaatg ttgtctttaa aaagattcca agttataaaa
     2941 tcatatcact tagagaggta gtcccagact accaatctga aggtatactc tgggaaaagt
     3001 tatctaaatt tattaaagaa gaacatatag aagtatcacg tcaacccaac aataatatag
     3061 ctttttacca tgatgaagaa gtcaaagata atggtgtaga tattgaagtt ggaatggttg
     3121 taaagaagat tggaaagaac aaatctggat ttatatatcg tgagacagaa gaaattgata
     3181 tgatggcatg tactatggtt tatggtccat atgaaaatat agctggtgca tatgaatcgt
     3241 tctgttattg gttagataaa aatagtgatt atcaaatatc aggtataaat agacaaatcg
     3301 gtcataaggg tgagcataat gaaataaatc ctgaaaatta tcttacagaa attcaaatcc
     3361 cccttataaa agtctaatcg attagtactt aaatacttaa gtactaatca cccattgaca
     3421 tcccaaaatt ttcatgatag tatctaaacg tgtattaaac atgtttaatt tttttatgga
     3481 gaaactttta aggggaaaaa ttatgaaagc agattcaaca aaggaacaat taattaatgc
     3541 cacaatccag ttactatctt cacatagaga tgtttcaagc attacggcta gagaaattgt
     3601 agctaaagca aatgtcaatt tagctatgat taattattat tttaaatcta aagaggagtt
     3661 aattaatatt tctattaaaa aatttttaaa gcgtcatatg gatgaatata aggctacaca
     3721 tgatactaaa aatttagcac ctaaacagca attaagggaa atactaattg cattttttga
     3781 ttttataggt gaacatagcc aatttataaa gatttctgta ccctatatat tgatacaaga
     3841 agaaattgtt tatccatttg agatactacc tttaattaag gctcactatg aaaatcagaa
     3901 taacaaaaaa actgaagctg aatacagaac tattgcgtat caaataactt catttattca
     3961 gttgcttttt cttcgttcta atgctttttt caagtatagt ggtattgata ttcttaatgt
     4021 tgaacagaga aatggtttaa tagattttca attagattta ttattggtag atattacaaa
     4081 ataatactat attaattttt tatgtacatt tttttatttt atggtaacgt ttgaaaatat
     4141 tctcattgac taaaaatact tgcattaaat aagggggagg aattttaatg ttttttacat
     4201 ttataggagt aatatcagca attttattgt tgtgtacaat tatatgtggt ttatggataa
     4261 aacttaataa tcgtaataat gactctaatt ctcactttac tttttcaata ctttcaattg
     4321 ctttttcact gttaattgtg gtggcctttt tagtaagaaa ttaatgtctt tgttatgctg
     4381 gggtttatta ttatagattt ttattattgc atgattttta ctatattata gtatttttat
     4441 tatattacag ggtcttattt attatagtgt tttgttatta cagcatttta ttatattaca
     4501 gagtcctatt tattatagga tttttattat attgtaaaat cttatttatt ataggatttt
     4561 gttatattac agacttttat tatattacaa tctcttattt attgcagtgt tttgttatta
     4621 cagtatttct attatattgc aaactcttat ttattatggt gttttgttat tgcaacattt
     4681 ctattatatt ataaagtctt atttattaca gttctttgtt atactacaga cttttattat
     4741 tacagcattt ctattacaat ctcttattta ttacagttct ttgctatact acagaccttt
     4801 attattgcaa catttctatt acattgcaaa gtcttattta ttacagttct ttattatact
     4861 acagactttt attattacaa catttctatt atattacaat ctcttattta ttacagttct
     4921 ttattatact acagactttt attattacag catttctatt acattgcaaa gtcttattta
     4981 ttacagttct ttattatact acaaaccttt attattacaa cattttatta ttttaacgtc
     5041 tttactataa tatagtaata ttttaatatc tcaaaatcat atcaatcact tttcagcata
     5101 gtctaaaata cttccacttt ctaaaattat ctatcatacc tatactaata atacttttta
     5161 acctttttat tatacatttc aattgttttt aacgacctat ttatttccaa aactccaata
     5221 tattttctca attacattgt gttaataacc cctaaatcct ttcaaattat ttacaataca
     5281 ttccattaaa taatcttact gctctataat tctttttttc tttttaaccc tactatacat
     5341 tatttacatc atttttttca atacaacatt cgttattcta taaccttttt acttttacta
     5401 tatacctttt ttctaactac accttcaatg ttccacatca tattacttta gcacaacaat
     5461 taatacctaa aaataaccct ctaaatcctc caataatata atcaatttaa ctataataat
     5521 aacctctcac actatccaaa caatatattt tcaaacatta caaaaaaaca tccaactatt
     5581 acaatattgc tcatatccct cctccccttt caaatgctat cattgactta caagataaat
     5641 gaaagtgagg atgatatata tgcttaaagt aaaccaatta tgtaaatcat ataaaacagg
     5701 caataaaaca tatcctgtat taaaaaatat ttcttttgaa atcaacaaag gagagtttgt
     5761 cgcaatcatg ggttcatcag gaagtggaaa aactactctt ttaaattgta tttcatgttt
     5821 tatcccaatt gataatgggg aaataattct tggaaataat aatataagaa atttaaaaga
     5881 aaaggaactt tcagaagtta gaaaccaaca actaggattt gtattccaag atttcatgct
     5941 atttgatggt cttagtgtct ttgaaaatat atgtgttcca caaattatca aaggtagtca
     6001 agtcttacaa atggaaaacc gtgcaattaa tctatgcgag ctatttggta ttgaacatat
     6061 cagaaaaaaa taccctgccg aaatatctgg tggagaaaaa cagcgtacag cagtagcaag
     6121 agcactcatg aacaacccag atttaatatt agcagatgaa ccaactggta accttgatag
     6181 taagtcttgt cgtacagtca ttgaatcatt tttaaaagca aagcaagaat tagatgctac
     6241 tatactcatg gttacacacg atagctttgc agcaagcttc tgcgaccgag ttattgtgct
     6301 taaagatgga ataatttata aagaagttgt aaaatcaaat tcaagacata aattcctaga
     6361 tgaattacta gaagttttaa aagaaatggg aggcgctcaa gatgacaatt aataaattat
     6421 acagcaaact tcgagcctat aattggaaga actatacaac tttattcttt tgtataatac
     6481 tttctgtggt attagtaact tcatatgctc taatttattt tagcccaaca atacaaaaca
     6541 tacttccaac tggtggagat agcagaaaac aggctacaat gatattctca gttgctatat
     6601 taggatgcgc tgtctttact acatacgctt cttccctctt tttaaaattt aaaagcaagg
     6661 aaattggtat tttcatggct ctaggaacag ataagaagaa attaaaaaaa cttctatttt
     6721 cagagatatt gttaatttca atagtatgct gttttttagg tcttatatta agtattcctg
     6781 tatcttttgg tatatggaaa atatttcaat tagtgattat tgataccaga gaaatgacat
     6841 atcattttgg aactcaagga ttcatatatg gatttatatt tagtgtattt gtgacattat
     6901 gcatatttat actttctgta aaatttatta atcgtacaaa tataattgat atattaaatg
     6961 accagagaat atctgaacca atacaggatg taaaggactg gtatggtata ttaggtataa
     7021 tttttattat actaggtatt tttctaggat atatcgtacc ttcactaact ataaacaaat
     7081 taaattatag actaccatct gtatggagct ttacttatct actctctctt attggaatat
     7141 atatgattat gacatacata gttgtacatg ctaaaagagg aaaacatcca gaaaaatact
     7201 ataaaaacat catatcaact agcatgatga gatttatggg aagacaaaca gtaaaaaaca
     7261 tgtgtgttat ttcatttttg attgcaggag cattatttgc tgtattctat gttcctaata
     7321 tggttgcagg tatctatgat actcttaaaa ataaccctat agattatact tttacctaca
     7381 atcaaagaga taaacaggta tcaaaaaaag atattgaaca attagcagat aaacacaata
     7441 taaaaattca aaactatatg gaaattcctt ctatagaatt aattgttgat ggtgtagaat
     7501 caatatatca taaagatgga actatcgaaa gggtatatat gaaaaaaagc tcctatgata
     7561 tgttctttaa ttctaaagat ttttccaagc taattgacaa accaataact ataaaaaatg
     7621 gtgaatatct tgctgttgta aataaaggaa atgaaaatca agattataaa tataccaaat
     7681 ttatatcaag cccagtcaca gaaaaagaaa tcccctttag atatgctgga aaaataatat
     7741 ttaatggttc tttttttcaa aatagaagta tgaacgccta tgtccttaat gatgaggatt
     7801 ataaatcatt tactaaagat ttatccataa aaaattacaa taattctata ctatttaatg
     7861 tagataaaga tacctatgct tttgccaatg acttaaagaa agaaattata aaaagaacat
     7921 ctaaggaaat gggcatgttc agtggttatg atgaatacgt aaaaaaacgt tatgaatcaa
     7981 ctggaagaaa atattttatg gatgaagaat atccaagtgg tgaaggtcat ttaaaactag
     8041 acccaaacaa caatcagttg tatcttaatt ggaaatacta tcctaacttt aaaattttac
     8101 aaagccaaga tatgataaaa aacatggcag tcttcttaat gttattcata tatgtggcaa
     8161 tcatatgttt tacagcagta ggaatcattg catatacaag gggtgttact atagctataa
     8221 atcacaagca ggtctttatg gatttaaatc gattaggagc aaacaatgat tatataaagt
     8281 tctgtattag aaatcaatta aagaaagtat tttcataccc tcttattgca ggaagttttt
     8341 taatttatct gtttaatttt atgattatgt taaataatga tggtagaatc gtcaatagtg
     8401 aggtcaatgc tcttttgata aacttaggtt ttatagggat ttgtggagtt tatatgtacc
     8461 ttatatatct attaacttat aagaaattca aaaaaattat tggtatttta taataaggaa
     8521 ggttgttaga tttttaaagt aataaaatca ttacttttta taataagaaa agcattagca
     8581 ttttaaatta aaaaaggtta tt
//
LOCUS       DAGQWR010000163         8472 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.164, whole genome shotgun sequence.
ACCESSION   DAGQWR010000163 DAGQWR010000000
VERSION     DAGQWR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.164"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(137..1168)
                     /locus_tag="MIW95_003350"
     CDS             complement(137..1168)
                     /locus_tag="MIW95_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBY3152648.1"
                     /translation="MLKKKSSLTLKTIREILPGLFISVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGAIVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            1356..2246
                     /locus_tag="MIW95_003351"
     CDS             1356..2246
                     /locus_tag="MIW95_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY3152649.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSQKYPDIYVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYNSHLLKDFSFDKLQDQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            complement(2298..3224)
                     /locus_tag="MIW95_003352"
     CDS             complement(2298..3224)
                     /locus_tag="MIW95_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HBY3152650.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFNYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIGKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYILSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVENSLHRILEHSKEGRTSMLQDVEAHRPTEVDMF
                     SKNICKLGKKYSIPTPINQTLFYMIKVIESRF"
     gene            complement(3439..4023)
                     /locus_tag="MIW95_003353"
     CDS             complement(3439..4023)
                     /locus_tag="MIW95_003353"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBY3152651.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            complement(4108..5010)
                     /locus_tag="MIW95_003354"
     CDS             complement(4108..5010)
                     /locus_tag="MIW95_003354"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBY3152652.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            complement(5003..5695)
                     /locus_tag="MIW95_003355"
     CDS             complement(5003..5695)
                     /locus_tag="MIW95_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBY3152653.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            complement(5734..6654)
                     /gene="pyrB"
                     /locus_tag="MIW95_003356"
     CDS             complement(5734..6654)
                     /gene="pyrB"
                     /locus_tag="MIW95_003356"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBY3152654.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            complement(6950..7972)
                     /locus_tag="MIW95_003357"
     CDS             complement(6950..7972)
                     /locus_tag="MIW95_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBY3152655.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
BASE COUNT         2718 a         1527 c          904 g         3323 t
ORIGIN      
        1 ccttaaataa aaaatgaatt tctataagtt tatacccact taaacttatc gaaattcatt
       61 ttttatttat attataaatt attagtatca tattatttta attaatcaaa ttaatattac
      121 aaatactatt tttgtgctat ataaatattt ttattaaaac tacagctgat acaacttgta
      181 ctgtacctac aaataaacca tataaagaaa cttctttccc ttgtttcact aaatctttta
      241 cattaacttt taatccaatt gctgctaaag ctattatttc tagtttgtta cttatttctt
      301 tgcacaacac agatacttct tttggtataa tgttcattga aaaaagtgca caagttataa
      361 agaatccaat tacataccat ggtatagtta cttttttctt ttttatctca tccttttctt
      421 cttcaaggat ttccctatta gactgatttt tcatatgtcc aaatactaat acaacaacaa
      481 ctagaaatat tactcttact atcttaaaaa tagtagccaa atcttttaca ttttctccaa
      541 caattgctcc acttgctaca acctgaccaa ttgattgcaa agttccacct atcattgctg
      601 aagtttttac aagttcatgg ctatataaaa attgagatat tattggaagt aaaaacataa
      661 ggaatatacc agtaacattt acaatagtta ctgcaattcc cttttcttta tctgtcgcat
      721 taaccactgg aactattgct cctatggcag aagaaccaca tactgcattt ccacttgcca
      781 ttagaaatct aaagttttct tcaaatccta gttttttacc tatataaagt gcagctatta
      841 ttgttattgt catttgcaat attataaata aaattcctga aacttttaag tctattaagg
      901 tagatacact gagtgttgcc cctaatagta ctattgaata tgataacaaa tctgtctctg
      961 aaaatttata acctttttga aatacctttt gatttaaaaa taggtttcct acaaacatac
     1021 ctaaaaatat agaaattgat gctgccccta ccttaggaat taaaatagaa ataaatatac
     1081 taatataacc tactaacact gatataaaca gtcctggtaa tatttccctt atagttttta
     1141 atgtcaaact gctttttttc tttaacataa tatacctacc ttataattaa tatagttttc
     1201 ttcatagcaa tagtatataa ttgtaataac cataagtaaa ataaatatat aatatcgtaa
     1261 tgattaatgt ttttaatggt tattatatat agtataatta tctgtagaaa gtatataaaa
     1321 aatgtaaaat ttcattaggt gaaggagaca aaaaattgtt tgaagaatta aaaacatttg
     1381 tagctgttgt tgaatacaaa aactttacta aagctgggga atacttaaat ctatcacaac
     1441 caagtgtaag taaacacata aaaaatctag agaattattt taaagttgtg cttataaaca
     1501 ggtctattaa acaaaaaacc atttttataa cagaaagtgg acaaatattg tacaaaagag
     1561 ctaaagaaat attaaatctt ttaaatatta cttatcatga tgtaagccaa gtttcagatg
     1621 ctataactgg tcacttaaag attggtgcaa gtttaactat tggagaatat attctaccaa
     1681 attttttagc attattttct caaaagtatc ctgacatata tgtagaagtt tttataaaaa
     1741 atacatcaat cgtatcttct catgttaagg attatatttt agacattggt cttattgaag
     1801 gcacttgctc ttccccttca tttattcaag aatatttttt tgaagataag atggttttag
     1861 cacttccata caatagtcat ttattaaaag attttagttt tgataaactt caagaccaaa
     1921 aatggatagt cagggaggat ggttctggaa ccagagacta tttagatatg tttttaagtg
     1981 ttaaagaaat tattccaaaa agtatgatgg tttttggaag taattatgct gtcaaagaat
     2041 ctgttagaaa taatttagga attacaatag tttctaatct agtgacaagc ttacctgttt
     2101 taaacaatga gctgtctgtc atagagcttg gaagcagcta caacagacat ttctcatata
     2161 tatttccaaa agatataact ttgtcaaaag cagcaactat tttcattgaa gagttaaaaa
     2221 tatttagtaa cttaaatagt atataacatt aataaaattt aaaatcaata attatcaata
     2281 tttcttacaa atacccttta aaatctactt tcaattactt ttatcatata aaataaagtc
     2341 tgatttatag gagtaggtat gctatatttt tttccaagtt tgcagatatt cttagaaaac
     2401 atatctactt ctgtaggtct atgagcttcc acgtcttgaa gcattgatgt tcttccttct
     2461 tttgaatgtt ctagtattct atgtaatgaa ttttcaacat catcttctgt aagagatatg
     2521 tcttttgctt gtgctatagc aacaacttcc ctcattgcag attttgctaa ttctcttaaa
     2581 tgctcagaac tctgaaaaac tccataaggt gcaccaagta tagctgaagt ttgatttaca
     2641 ccaatattaa ccatgtactt ccaccacata tctcgttgaa tatcttttga taaaatatat
     2701 tctatattta catcatcaaa gacttcagtt attatattag tttttctatc ttcactatta
     2761 tctttgttac caaatacaat tatcccatta gtagtgtgta taatattatt attgattttc
     2821 tttgcatcta tattggtaac atatgaatat accatttttc caattccaaa tctttctcct
     2881 attatctctt cgctatcaac tccatttagc agagacatta taactgtatt ttttccaact
     2941 aatccatcta attcttttat attttctttt aaattattat attttagacc tataattata
     3001 aaatcagcct cttgtttata ctcatcttta gttacataat taaaatcata cctttttcca
     3061 tttattataa atccttcttc taaatatctt ttctttcttt cattatcaca aagaatttta
     3121 aaatcatatt tagagttaaa aaattgttct gcaaaagtag ctcctacagc acctactcca
     3181 aaaaatacaa cctttttatg attttcattt aattgatttt tcatacttac ccctccctaa
     3241 tatataatac ttttacctta tacttatatt gtactgctac tatctcttat tttgtaaata
     3301 tatttttaac aaaaaaaaca gctaaccttc atttataagg ctaactgctt tttattttta
     3361 agttatatgt tatattacaa atttttactc taaaaaattt acatttttta agtaacacta
     3421 taaataatta agtttttatt acatccctaa ttccttgaac tctctacttc caggcttaac
     3481 aactggtaat tttccttcct tacataaagg acactcatca gattcataaa cttgaatatc
     3541 aagttttata gcactatata taggcatacc tatatcatga ttagttctat ctgctataca
     3601 tgcaacacct acaacttctc cacctaaggc ttctaatact ctttttgtct ccatagttga
     3661 tttaccagta gttacaacgt cttcagcaat tattatcttt gctccttttt taacttcaaa
     3721 tcctcttctt aactccattg tattgtcttt tctctcagta aatacagctt cttttcctaa
     3781 ttgtcttcct aactcataag aaactattac tccacccata gctggtccta ctactaagtc
     3841 tatatctaag tcttttattt gttcaacaac tgtacttaat acctcagcag catattctgg
     3901 aaatcttaat acttttgcac attgtacgta tctattactg tgttttcctg aagataataa
     3961 aaaatgtccc tccaacaatg catcactttt ctttaatata tccactacat ctactttact
     4021 catattattt ttcctccatg ttaatgttat aaaattattg tttatttaat ctatattttt
     4081 tatctttaaa atactattta ttctacctta tataattcct cttatttcgt ctaaactttt
     4141 tattccttct ctatccataa attcattaat cccatctatt atttcaatac ctattctagg
     4201 attcataaaa tttgcagttc caacttggat acaagtagct cctgccatta taaactctat
     4261 agcatctgtg gctttagtta ttccgcccat tcccattaca ggtatattta catttttaca
     4321 cacttcatgt accattctca aagctatagg ttttatagct ggtccagata atccagcata
     4381 tacattttca aatactggtc ttctattttt tatatcaatt gccattgctt taaaagtatt
     4441 aactaatgaa actccatcag caccttcttc ttcacaaact tttgccattc ctacaatatc
     4501 ttctgcattg ggtgataatt ttattactaa aggcagtttc gttatgtttc ttacctcacg
     4561 aacaacttct cttgccactt cattttttat accaaaagcc attccaccag ctttgacatt
     4621 tgggcaagat atatttaatt caataatgtc tataggcttg tcatttaaca tttgaacccc
     4681 taataagtag tcttctaatg tcccacctcc aacattagct attctgacta agtctagttt
     4741 actaaaaaaa ggtaattcat agtctataaa tccttgaact ccagggtttt caagcccaac
     4801 actattcatc ataccggatg gtgtctcatg aactctcata ccattgttac caggtttttt
     4861 atttaaagta agacccttgc tacttatacc accaagtttt tgtatatcat aaatctcatt
     4921 atattctttt ccaaatccaa aagttccaga cgccattatt actggatttt taaattctac
     4981 acttccaaat ttcacactta aattacccat taaaaatcac gtcctctccc cagaacactg
     5041 gaccatcttt acaagtcttt ttgtttcctc cattagtttt acacgtacat actaaacagg
     5101 cacctactcc acaagccata tggttttcaa gagataccat aattctcgtt ttagtacctt
     5161 ctaccatctt aactaatttt tccatcattg gagttggacc acatgttaat atataatcat
     5221 atttctcaac atcaattata tctgttacaa aagtatttcc tactgttgta tagactttat
     5281 tacatacttg cttatattca tcttctaata taacatcttc tctaaaccca agatatgcat
     5341 cacaattttt tatattttta gcaaccaaat atagtggagc tacaccgatt cctcctccta
     5401 ctagagctac ctttccatca accttagcat atccattacc ataagggcct tctaatttta
     5461 tattatcatt tacctttagg ttactcaaaa tttgagttcc ttctcctact actttataaa
     5521 gaaaagttat cccttcttca tctatatcat gtatacttat aggtcttgaa agtactggat
     5581 aagtatccca tgctcttaac atatagaatt gtcccatttt accttcaaaa ttacctttta
     5641 ctttcattct atacatatct tcgcctatat atatattttc cagaatttta tacatcccaa
     5701 aatctccttt gttttactat tatatgataa ctattagtct tcatttatac ctaaaagttt
     5761 tattattaaa gccattctaa catacattcc atatttagct tgtttaaaat atacagctct
     5821 atcatcacta tctacatcta tgtctatttc atttactctt ggtaatggat gcattactaa
     5881 catatcttta gatgcctttt ctaatttagc tttatttaaa atataatagt ttttaagtct
     5941 ttcatactct gatttatctt caaacctttc ttgttgaact ctagtcatat ataaaacatc
     6001 caagcttcct ataacatcat ccaaattatt agtttcataa tatgcatgtc cctttatagc
     6061 ttcttttata taatctggca tttttaactc ttcaggcgct ataaatacaa actttgtatt
     6121 tttatatctt gccatagcct taaccaaaga atgcacagtt cttccatatt ttaaatcacc
     6181 acataatcct attgtatggc tttctaaagt tccttttaat gattttatag taagtaagtc
     6241 tgtaagtgtt tgagttggat gctggttttt gccgtctcct gcatttataa atggcacttc
     6301 cgtatatcta gctgcttctg aagctgcacc tgattgagga tgtctcatag ctattatatc
     6361 aacataacca gatactatcc tcattgtatc tcctagagtt tcaccttttg atgcagagga
     6421 tgaatttggc tctgaaaagc ctactacacg tcctcctaat ctgttcatag cagattcaaa
     6481 gcttaatctt gttcttgttg atggttcata aaataatgta gctaatagtt ttccttcaca
     6541 tatacgagaa tattttgacg gattgtcaat tattttttga gctaattcta atatttcatc
     6601 tatttcttct attgaaaagt cttctggttg gattaaattt cttgatttta acatctttcg
     6661 tcctcctttt ttagcctctc tgaactaaaa ttaaaagtat ttttgtactg ttagtagctt
     6721 accaccttta ccctatcctg tcaagggatt gcgcaaataa tcatcatatg aaaaggctcc
     6781 tatttaatag tcatattaac ttgaatcttg attagattta aagtgataca aaaacgctct
     6841 atagtacatt taaaatataa tcattttttg tttccatgat taaattaaat ataatataga
     6901 gcgtttatat atatttatgt atttatcaat ttgtcatatt acaaaccgat tataatattc
     6961 tttttgctgt aacaaatctt gctgtatagt atgatgaatt tatactagtt acactaactg
     7021 tttttcctgt tgatggtgaa tgtatcatat tacccccacc tacatatatt ccaacatgac
     7081 taacagaacc agaaccatta gttgtgaaga atactaagtc tcctggttgt aaatttgctt
     7141 tacttacagt ttgtccatat ttagattgtg ctttagatgt tcttggtata ttctttccta
     7201 ctgcatttct atacacatat tgtgtaaatc cagaacagtc aaaagtgttt ggtccttctg
     7261 ctccccaaac atatggacaa ccttgcttag aatatgccaa gttaagtaca gcttgaactg
     7321 cattagagtt agctgttggc acatttgaat tattattttg ttgattagta tttgcattgt
     7381 tttgttgatt attatttgta gaatctacat tttggtcaat acttgggttt acttgtggct
     7441 cttctattac aggagcttta tcagatatat aagatgattt tacatatcct tctgtttccc
     7501 catctttaac ttttaaccaa tctccattat cctccaaaac caataaagaa cttccttttt
     7561 ccagctcttc aattacttct gaactttcac tcttatcttt tcttaagttt actttatcag
     7621 cgttagtata tcctacacct tcagatatat ctacatatct agtagctaac caaccttcgc
     7681 catcttcaac tttaacctta acccattctc cttgagttcc taaaaccgta aattcatctc
     7741 cagaataagc tatgttttga acttgacctt cttctcttat ctttatagct actccatctt
     7801 ttatttttgc aactttatat tttacttctt tatattcacc taaatttaat tcagtatttg
     7861 ttttatcatc cttattttga gatgaatcat ttacttcatc tgcatttaca attgaactta
     7921 ctgataatgc cataactgat gcaaatactg gtattaatat ctttttcttc acgataaacc
     7981 tcctaaaatt ttcccataat tatcttatga ttctattttg taggtgaagt gtacctaagg
     8041 ataaaatact aatacacaat acattaataa aagctctatc tgtaactttt ttgtaaacca
     8101 ctttatataa cttttatagt atcattatat taataaaata aataaattag cttatccaat
     8161 atgtcaccta aatatacatt atttacttaa ttatataata tgggtctgta aaagtaaaga
     8221 attttgtacc attcgaggtt aaagtgcgca taaatattgt tttatgtgtt tttttatgcg
     8281 cttttatatg gcgtatattt tttgatggat ttagtataaa gttagatatt ctgtgaaatt
     8341 tttaaccctg cataaatcaa aacctatttc ctaatagtga ttctcaaatt ctaataattc
     8401 taagttaaaa agatgaaaat tttggagaat ataaatatta aaaaattaag tatgaaaaga
     8461 ttgtcctagc ag
//
LOCUS       DAGQWR010000164         8401 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.165, whole genome shotgun sequence.
ACCESSION   DAGQWR010000164 DAGQWR010000000
VERSION     DAGQWR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.165"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            180..1166
                     /locus_tag="MIW95_003358"
     CDS             180..1166
                     /locus_tag="MIW95_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBY3152656.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPMVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1389..1472
                     /locus_tag="MIW95_003359"
                     /pseudo
     CDS             1389..1472
                     /locus_tag="MIW95_003359"
                     /inference="COORDINATES: protein motif:HMM:NF012587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1503..2984
                     /locus_tag="MIW95_003360"
     CDS             1503..2984
                     /locus_tag="MIW95_003360"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBY3152657.1"
                     /translation="MYKEPKYRTILESIKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSVLEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3010..3981
                     /locus_tag="MIW95_003361"
     CDS             3010..3981
                     /locus_tag="MIW95_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBY3152658.1"
                     /translation="MKKVISIGEALIDFIPNQNGCKLKNVSEFRRVAGGAPANVSVVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFMTGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4237..4839)
                     /locus_tag="MIW95_003362"
     CDS             complement(4237..4839)
                     /locus_tag="MIW95_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY3152659.1"
                     /translation="MEICILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEKHSRQFAKQIYYILDNSIEVDNC"
     gene            5120..6331
                     /locus_tag="MIW95_003363"
     CDS             5120..6331
                     /locus_tag="MIW95_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBY3152660.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKYDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIVFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6399..6596
                     /locus_tag="MIW95_003364"
     CDS             6399..6596
                     /locus_tag="MIW95_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBY3152661.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6630..7601
                     /locus_tag="MIW95_003365"
     CDS             6630..7601
                     /locus_tag="MIW95_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3152662.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7788..8165
                     /locus_tag="MIW95_003366"
     CDS             7788..8165
                     /locus_tag="MIW95_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY3152663.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3410 a          872 c         1353 g         2766 t
ORIGIN      
        1 aatataacaa caaaaaatga aaattacaat taatttaatg ttaaagactc aattatttgg
       61 atgtggaatg gatagtacaa gtagtatata aaaagtattg actggacaaa taactatatt
      121 ttattttaaa tatataatat aatgtaataa aatatatcaa atgtagaagg tgataaccta
      181 tgaaaaaaat aacaataaat gatattgcta acttggcagg agtatctaaa agtactgtat
      241 caagatatct aaataataaa gatatcagtg attctactaa agagaaaata aagacaataa
      301 tagatgaata tggatatgag ccgaatgcat ttgctcaaag tttgagagct aaaaaaacat
      361 actttattgg tattataaca ccgtgtttag attcttttgt aaaatctaaa attatgatgg
      421 caatagatga agaactcaaa gaattaaaat atacatcact aattattaat actaatagaa
      481 aaataaggtc agaaatagat agtatatcaa agttagctag tctaaaagtt gatggaataa
      541 tactaattgg taccgaaata actaaagaac ataagaatga aattgaaaag ttagatatac
      601 caatggttgt agttggacaa aaagttgatg gtatcaatag catagtgaat gatgattatg
      661 gagcaggata taaaatgggt caatatattg ctaataaagg ttacaaaaac attgtatatt
      721 taggtgttga tgaaagtgat atatcagttg gtttaaatag aaaaaatgga gtacttaatg
      781 ggttaaaaga taaagggtat gatgcaaaag tattttatac tgactttgac caagaaacgt
      841 ctatacaaag aagtggagaa atgctagaaa gtgaaaatcc agatataata atatgtgcaa
      901 ctgataatat agcaatagca acaatgaaag aaatcaataa gagaggtaaa aatataccac
      961 aagatatatc tgtagctgga tttgggggat atgatgtatt gtctattata agccctaaac
     1021 ttacaacaat aaagtttgaa aataaaaatg caggtaaagt agcagcaaat acgatagtga
     1081 acttaataca agaaagaaaa gaaccactat taaaggagat taaatttgaa ttaatagaag
     1141 gtgaaagtac aataaataaa aattaagata cacattacat atattagtaa tgtgtatttt
     1201 tttactaaaa aaactataat tctaaaaatt aggaaaactt ttttgaaaga aatattgcat
     1261 ctgaaataaa tttatgctaa tctttaatta aaccggtttc gaaaccggtt taattaaacc
     1321 gaaagaatag cacaatgagt gcaaaaataa ctgattgtaa caaagtatca aagtaattat
     1381 tagagtacat aggaaataaa caaaatataa aaggtgtagc acattgtgta acaagattaa
     1441 gaattgtttt agatagtaat aataaagcaa atataaaatt acaaaagaaa aggaataaaa
     1501 atatgtacaa agagcctaaa tatagaacca tattagaatc gataaaagaa gaattaaata
     1561 aattaagaaa tatatcttta aatgataaat ataaaccact atttcatata catccacaac
     1621 atggactact taatgatcca aatggattag catattataa tggtaagtac catgtatttt
     1681 atcaatggta tccatatgat gcaactcatg gaatgaagca ttgggcttat gtaagttctg
     1741 atgattttgt aaactggaat agagaagatg tagcattaat accaatcgaa agttatgaat
     1801 ctcatggagc atattcaggt aatgcgatag aggtagatgg aaaattgcat atgtattata
     1861 cagggaatat aaaatatagt gcagaagata gatatgcata tcaaaactta gctataatga
     1921 acaaggatgg taaaataact aaatatgaaa ataatccaat agtaagtgaa ataccaaaag
     1981 gatatacagg acatgtaaga gacccaaaag tatttaaaag aaaggataaa tactttatgt
     2041 tacttggagc acaaacaagt gataaaaaag gagttataat agtatatgaa tctaaaaact
     2101 ctatagattg gaattttaaa ggagaattaa atgtaaaaaa tatagatgaa gattttggtt
     2161 acatgtggga atgtccagat tatattaata tagatgaaaa agatatatta atattttcgc
     2221 cacaaggagt agaacctaaa ggatttgatt atcaaaatat atataatgta gtatatgcta
     2281 ttggtaatat ggatttagat aatttaacat ttgaaataga tacaatgaaa gaactggaaa
     2341 aaggatttga tttctatgct ccacaaacat ttataaagga ctcacaaata atactatttg
     2401 catgggctgg aatgggagaa gttttatacc ctacagataa aaacaaatgg gcacattgtc
     2461 taactgttcc aagaaaatta aatataaaaa ataacaagct attgcaaatg cctgtagatg
     2521 aattaattaa attaagatat gatgaaacaa gtgggcaaaa tactataaag aataatatca
     2581 atattataga aaatgatgaa aatgtatatg agcttaatat taatataaaa aatattgaca
     2641 gcaataaatt tgggctggaa ttattttcat cacaagatga aggtgtaaaa ttagaattta
     2701 acaaattagg aaacattgta actttagaca gaagcaactt taaaaaagtt ttcggtgttg
     2761 aatatggaac aaatagaaaa gaatatataa atattgatga aaatactaat atcaaagtac
     2821 tagcggatag aagtgtttta gaaattttta taaatgatgg agaagtagta tttactagta
     2881 gaatatttgc taaagaaaat tctaatcaaa taagggttta tagtgataaa atagtagagt
     2941 atgagtatac taaatttaaa ttaaagcaag gtattgaatt ataaaaacac attataaggg
     3001 gtagaaaaga tgaaaaaagt aattagtata ggagaagctt taatagactt tatacctaat
     3061 caaaatggtt gtaaattaaa aaatgtatca gaatttagaa gagtagcagg aggagctcca
     3121 gcaaatgtta gtgttgttgt agcaaagctt ggaggaaaat caagttttat atctaagtta
     3181 ggaaaagatg catttggtga ttatataata gatgtattaa atgaagtaaa tgtaaatact
     3241 gattatgtat tgagaacaag taaagcaaat acaggacttg cttttgtatc attaaaagaa
     3301 gatggtaatc gtgatttctc attttataga aatccaagtg cagatatgct tttagaagct
     3361 gatgaggtta aaaaagagtg gtttaataat tgtcatatct tacatttttg ttcagtagat
     3421 ttaatagata gtccaatgaa attagcacat aaaaaagcaa tagaatacgc acttgaaagt
     3481 aatagtataa taagttttga ccctaatata agattacctt tatgggatag tgagcaatct
     3541 tgtaaaaagg caatatcaga atttttacct tttgctcata ttgttaagat atctgatgaa
     3601 gaattagaat ttatgacagg agaaaatgat atagaaaaat cattacataa actctttgtg
     3661 gggaatgttg aactagtatt atatacaaaa ggaaaagatg gagtagatgc atacactaaa
     3721 aaagtaaaag gtatgtgtaa aggggttaaa gtaaatgcaa tagatacaac cggagcagga
     3781 gattcttata taggttcatt tttatacacc ttattgtata aacaaataac attgaatgat
     3841 ataaaagaaa tgaaacaaga aacgttaaat gagtatttag aattttctaa ttattatgca
     3901 gctcgtagta caactattaa aggtgcgata agttcatatg caacaaagga agagataact
     3961 gaatttataa aaaatttata gtaggaatga gtaataggtt ttgaaggaag taacatgtaa
     4021 agagttttta aggatgttta ctatgaaaat aattaacata taaaaagcaa taaagaggct
     4081 gtctcaaaat agatataagc ctctttatca ttgttgaaat gataaaaaat attttaatta
     4141 ttgttacttt ttagaagaat atatatctta ttttaaataa gcaaattatg taagatttat
     4201 ttatattatc aatactttac ttggagtatt atgtctttag cagttatcta cttcaattga
     4261 attatctaaa atataataaa tttgttttgc aaattgacga gaatgttttt ctttttcttt
     4321 attcataaaa ttaggtttac ctaaatcacg ttcaacaagc attcctatat attttaactg
     4381 tgaatgctta cagtagcgct tcataccatt ttcccaaata tcagcaccct tttcaggtct
     4441 atatccacat gttgtaataa gtgctactgg ttttcctgcc catagagcgg ggcctttctc
     4501 attaccatag tacttattca ttccatacac caatctgtca agtagtgctt tcattggaga
     4561 agtgcagtac caagaataaa ttggagtagc aaaaacaatt aaatcacaca taagaatttt
     4621 cttgaaaatt ttctgaacat catctttaca ataacatcca aaatcaaata agttatcctg
     4681 acatttttta caagcaatac atggctctat tttacaatca tataaccata ttacttcaat
     4741 atttgaatta taattttcta attcctcaat aaaaggtttt aaaatagcag cagtattacc
     4801 ttttttctta ggacttccca taagaatgca tatttccata ttgatacctc cacagtattt
     4861 taaaatagtt tttagtgctt aatatagttt taggatacta tgagaatacg ttaacaacaa
     4921 tacactttgt atatttttaa taaaattgaa acaaattgtt ttatcaataa aagttggaat
     4981 gtatctataa ttaaaaatat tttaggtatg tttttacata tatgtgtgta gttaactatg
     5041 gtaatttatt gtaaaattat cttataggat tataattgct taatttatga ttatatttaa
     5101 aatcttggag gtaaaaaata tgtactcaaa tgaagactat aaatttatag aagaaaatgc
     5161 accatgttat atatatgatg agactaatat aattgaaaaa tgtaaggaat tgaaaaataa
     5221 tattttaggt tttgattttt tatattctat aaaaacaaat ccatataaca aaattgtaaa
     5281 aagcattgca gagcaaggtt ttggagcaga tgcttcttca actaatgaag ttatgatttc
     5341 atcagaatgt ggagtttcta aatatgatat ttattattca tcaccaggca aaacagagat
     5401 tgatatagaa actgttttag ataaatgcat aattattgca gatagtatta cagaaataga
     5461 acttataaat aagatatcta aaaagcataa tcagaaagtt aaaataggaa taagaataaa
     5521 tcctgatttt actatggatg atgatagtgc taaatcaagc aaatttggta tagatataga
     5581 agatatgagc agtgttttta agatgttaag tatgtgtgat tatgtatctg tagttgggat
     5641 tcatatacat attaaatctc aggtattgga ttttgaaaag ttaggacatt attatgaaaa
     5701 atgttttttt atagctttaa atttaaataa aagtaagtat attaatattg aatttataaa
     5761 ttttggtagt ggacttggag tagcttatga taatcttaga gaaaatccac ttaatcttaa
     5821 agagctttca aaattaactc aaaagattgt ttttactaat tcaaacacac tgaaatcaag
     5881 acttcttatt gaatcaggac gtttcatagt ttgtaaatca ggtaattatt atgttaaaat
     5941 tgtagatatt aaagtatcaa gagggaaaaa atatcttatt gtaaaaaatg gacttaatgg
     6001 atttatgaga cctattattt ctaatttaat tgaaaatatt acttgtggag tagaattaga
     6061 aggtcaagag ccattatata catcaaatca tgccttttct ctttcagtat taaataattt
     6121 aaaagaagaa gagtatgtaa atgttgttgg gaatctctgt acatcattag atgtaattgc
     6181 aaataatgtt aaattgaaaa aagcaaatat aggagatttg atttcaatta gtaatgctgg
     6241 aagttactcc tattctttat caccactatt attttcgagt tatgaatttc ctaaacaata
     6301 ttataaaaga aaagatggat atattgagta aacaatagga taaattaata aacttagtat
     6361 tattgaaaaa atttaaaatg cagggggtgt aattctatat ggaattgtgg aaaagaaatt
     6421 tatttgtatg ttggataggt atgtttttta gttctatagg gatgagccaa atagcaccta
     6481 tacttccatt atatattaaa cagttggggg taactgatgt tagtttaata caacagtatt
     6541 caggtataat ttttggatgt acctttgtag tagcagcatt cttttcgcca atttgaggga
     6601 aagctgctga taaatatgga agaaaatcaa tgcttttacg tgcaagtatt gggatgggaa
     6661 tagttatatt taccatggcg tttgttcaaa atgtttatca gttattagga ttaagaatac
     6721 ttcaaggagt ttttacgggt tatgctactg catgtactac tcttattgca acacagacag
     6781 ataaaaatca ttcaggatgg gctttgggta ctcttgcaac tgcatcaact actggctcat
     6841 taattggtcc aacagtagga ggatatatag aatcaatctt gggtcttaaa agtacattta
     6901 ttataacagg aggattgttg tttgtatcat ttatcatctc tattttattt gtagtagata
     6961 attttaaacc taaagaaata aaagaatcaa tttctatacc taaagaacaa ttaaatatat
     7021 tgccaaataa atttttaatt gcatctattt ttgtaaccac attcattact caactagctt
     7081 tatactctat agaaccaatt gtaaccatat atatatcaca acttacaaat tttgcatcaa
     7141 atgtagcttt aatagctgga ttgacatttt ctgcatcagg attagcaaat ttattagcag
     7201 ctcaaaaact tggaaaaatg tcagataaaa taggtcctca aaaagtattg ttaatttcac
     7261 ttttatgggc tgggattata tttataccac aagcttttgt aaaaactgca tggcaactga
     7321 tgttgttgag atttttatta gggttgtcag ttgcagggct taatccttct gttaacagct
     7381 tgcttaaaaa aattgcacca gaagaatatg tgggaaaaat atttggatat aatgcatctg
     7441 cccagtatat aggttgttca tcaggagcat ttttaggggg acaaatatca gcacatttag
     7501 gtattagaac agtatttttt tcaacaagtt tattgttgtt tatgaatgca ctttggatgt
     7561 ataaatttac tggattgttt attaaagata agactaaata atcatactat ttacatttct
     7621 aattttatga tgtaaaataa atatttttta tgtaatttat catagaatta tataaaaaat
     7681 aactctatgg taaaaattgg aaaatattat tatcgacaaa atctttgact taaaaactat
     7741 attgcgatag tattaattta ataaaagttt gagaggggcg taaggttatg acaatttcaa
     7801 aaataccaca agcagaatta aaagttatga agtttatttg ggaaaaaaat gatacagtaa
     7861 catcaaaaga aactattgaa gctatggaac gaaaatatgg ttggaaacaa acaactacat
     7921 tgacactttt atcaagactt gttaaaaaac attttttaag tgcagaaaaa atagatagat
     7981 acactcatta tacaatagtt gttggacata aggagtattt aagtgttgag acaaaagatt
     8041 ttttaagcaa tatacacgac aattctttac agagtttaat ttcagcacta catgatgatg
     8101 aagttgttga taaagatatg ttggatttat ttgaaagtca ttttaaaaat ttagaagaag
     8161 agtaattata tcaggataaa actctaagaa tttaattaat ctatggctga aaaccataaa
     8221 tggtattatt taatcagcta ctcactattc ataaagtaat attaaatagg ttctctaatt
     8281 agtaaaatac ctaaaataat tggaataggg agtatcaagt taaatgatac cacttattta
     8341 aataagaaat gatttttaaa atttaaaata attttataac ttgtcaatat tcattaatct
     8401 a
//
LOCUS       DAGQWR010000165         8212 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.167, whole genome shotgun sequence.
ACCESSION   DAGQWR010000165 DAGQWR010000000
VERSION     DAGQWR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.167"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            52..621
                     /locus_tag="MIW95_003367"
     CDS             52..621
                     /locus_tag="MIW95_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152664.1"
                     /translation="MKLRNPKIKKLLSVALLAALIGIPIGQTANAMEISNNVPSYIEN
                     NPTTIEDLEINDSQLPKPGTVTYIDINKLIKNLKENGIDTTLIENELYYRKAGTNKIV
                     WTKTGFDLYLSKGVALTLAGAGTGVAGLLAGAIPGIGQYLAAAIVGAITMHLGSNITS
                     GKIFKFKKMRYNKGGAYVTKYYLQSVRNQ"
     gene            913..1230
                     /locus_tag="MIW95_003368"
     CDS             913..1230
                     /locus_tag="MIW95_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152665.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYISIGLFTRRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
     gene            complement(2216..2431)
                     /locus_tag="MIW95_003369"
     CDS             complement(2216..2431)
                     /locus_tag="MIW95_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152666.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVREGSSWAFNFIVQP"
     gene            complement(2614..2958)
                     /locus_tag="MIW95_003370"
     CDS             complement(2614..2958)
                     /locus_tag="MIW95_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152667.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            3085..3468
                     /locus_tag="MIW95_003371"
     CDS             3085..3468
                     /locus_tag="MIW95_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152668.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
     gene            complement(4677..4928)
                     /locus_tag="MIW95_003372"
     CDS             complement(4677..4928)
                     /locus_tag="MIW95_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152669.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(5174..5329)
                     /locus_tag="MIW95_003373"
     CDS             complement(5174..5329)
                     /locus_tag="MIW95_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBY3152670.1"
                     /translation="MALDVKIEVKIIKDVVILNIIHWKKETSLLTVKIARGQGDLSYV
                     KYFTNES"
     repeat_region   6546..7499
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6546..6574
                     /rpt_unit_seq="ctttacataccacttagttaatataaaac"
BASE COUNT         3044 a         1099 c          896 g         3173 t
ORIGIN      
        1 aataataata tatatctttg atatataact aatttaaaat ggaggtattt tatgaaacta
       61 agaaatccaa agattaagaa attgttatca gtagcattat tagcagctct tataggtata
      121 cctattggac aaacagccaa tgcaatggaa atttcaaata acgtcccaag ttatatagaa
      181 aacaatccaa caacaataga agacttggaa atcaatgact ctcaattacc taaaccaggt
      241 acagttacat acatagatat taataagcta attaaaaacc ttaaggaaaa tggtatcgac
      301 actactctta ttgaaaatga attatattat agaaaagctg gaacaaataa aatagtttgg
      361 actaagacag gctttgattt atatctttct aaaggagttg cattaacttt agctggtgct
      421 ggtacagggg ttgcagggct tttggctggt gccattcctg gtataggaca gtatcttgca
      481 gctgctatag ttggagcaat tacaatgcat cttggttcaa atataacatc tggaaaaatc
      541 tttaaattta aaaaaatgcg ttataataaa ggtggggctt atgtaaccaa atattactta
      601 caatctgtaa gaaatcaata ggtgataaaa tgattataaa atatctactt tttgcaatta
      661 tctcagcact agttttattc ttttgtaata aagtatttca actaagtaaa ttacacaagt
      721 tcattatatt gtttgtttca ttttttatac taacttttgt agcatcattt attaatagat
      781 aaaaaataag atggtggggt taacctacca tctttttctc atttaatatt tgtaatattt
      841 tagatactct ttatacttgt gttactgata tactttgatt aagaaaagta aatttaacag
      901 gaggtatctg ctatggattt tggaaatatc aatcttatac ttattggaat aatagttata
      961 ataggaacta caataatata tttaattaaa cctaaaactg ctttttgtag taaaaaatat
     1021 tttaataaat tagaatcaat atatggaaat atagataaga aaaaaactgt taaactagaa
     1081 gtactttatc gctatgtaac aggtcttgag tatatatcaa taggcttatt tacaagaaga
     1141 ttagatatta caataatagc aataatatta gtagctacta taacagtaat attatattac
     1201 ttagttagaa agagatatat aactatataa aatctaatag aattaatatc aaaagaaagt
     1261 agcaactaat caccagtaat gtaaaataat ttgtttttca aagttaataa aagtcttctt
     1321 tatggcaatg atttaaatat aatcaaatat cgtaacggag atttttttat gaataattta
     1381 agaaaacaat taatcaaaga aattatataa gatggagaac ttataaaatc tacagatata
     1441 agtagtttta ggagtaaata cagatgactt atttcaaaga gacaatagct acaagttatc
     1501 ctcattctgt aatacaaaaa tgtttaatac attagataag aaatacttta aatatgtttc
     1561 atacaaggac tcaaaagaat taataagtga ttttaaatct gtatataggt cagttaatga
     1621 ataaaaagcc ttatcgactt tagataaaat agattaaaaa taatgtaaac agtatacttc
     1681 agaaattaaa acgtttggaa ataactggaa tgttgtagca tcagccttta gttttactaa
     1741 aataagtaat atctataata tatattacaa gtataatcga aggatttaat aaatatttca
     1801 aaaatgtctc aaaaacaaag tccagattta catcaaacgc ttcactctta aagatattat
     1861 tcttgacaag cgaaaatgtg atgaaaaaat ggactatgaa atataaaaat tgatataata
     1921 tatacaatca tttattcata tcttttgata gtagaatata attttaaaaa ttatacccta
     1981 aaaatttgct ataattgtta taatatatat aatttccaaa aatatttaaa aacatctcaa
     2041 tatacatttt ttataatttg ttaaaataaa attcatatat taagaaatat taatagtaaa
     2101 taagcaattg ttactataac gtaggaatta tctttaaaaa cacaaattaa tttacattct
     2161 ctaattttaa agtatatgta tatctttgat tttaataaaa ttcacttaaa atattttaag
     2221 gttgaacaat gaaattgaat gcccaagaac ttccttctct tactaatgtt gcaattaatt
     2281 caaacccaca attgtattga actgaatctc tacctactgg tttccaacta gaaggtgcat
     2341 tatatgagaa agaatttgat gttggctctt tcttttgttg ctgcttaatt cctagcccat
     2401 tagaaataaa tccagtttga tttatatgca cccttctatt tgttacacga atctggccgt
     2461 ctgcaataga ccagtttcca gaaactttac taacggaaat atattgaaat gctccatctc
     2521 cttttgatat tgtatatgta actgtaccag atgcttttac accaccagtt ttatcccatc
     2581 catcttctga tatacttgct tttctaagag gctttattgt attagtatca aaaaaactgt
     2641 cttagaatat atctttgatg ttaaattttt agattgttta acttctaata attgagttgt
     2701 tgatatagaa tcaacgtata tggactctcc tgttcttgca tttttgatta tacctttttc
     2761 attggaactt ttaacatcag taattccatt tttagccata tcgtataatt tgtccatgtc
     2821 tgttacttgt ttctctgaat acattacttt catagatgaa tcttctacct tactagcact
     2881 tgcatttgtt ggtaatagta ttgtcattat gagtaaagat aaaatagttg ttacagattt
     2941 ttttaaccta taattcatat taatccttac tttttatttg ttataacaga cattctattg
     3001 taatagaata taaatccaca atatttttaa aattagtatt gaataactat tctcatgtta
     3061 taattattca aaggagggat tgatatgagg aaaaaatttt ttataggtat tcttatagtt
     3121 ttaataatct ctgtgatagc tatacttaat ataaaaaatg ataaaaatgt ctttattgga
     3181 gaatcagaaa attggaaagc taaatttgta actactgaag gacactcaaa attagaaata
     3241 aaatataaaa atagtaaaga tgtagttgaa caaaaattta attattccta tcaatttata
     3301 gatggtgaag atgcagttgt agttgtaaaa aatgaaattt tagaagcagg tcaagaaaaa
     3361 gcaataaaaa tacaaagacc aggtctttat tcaaatagag aatctagtta caagctaatt
     3421 atatcttgga atggaaatga ggaaactata aacttgaaaa agaaataacc atattatcaa
     3481 tttttaaaaa attattattc aaattcttat aaaaagagtg tttagaaaaa tgaaacactc
     3541 ttttttgtat aagtttttga aaactataaa tgaaatatat caaaatcgtt cgaaaaatta
     3601 ctgaaataaa ctcttaaata tattataaat taaggtatat tatacatgaa tttattggga
     3661 gtttatgagt tttaaaattg gaaaccaaac aataagggct aaaaatactt taaggaaaat
     3721 atataaagtt ctataaatta tcaaaaatat ttcgcaaaaa ttgataatta ttacaaataa
     3781 attgataata ctaataatat tagaaatatc gaaattgaac aaactaggat taaagctcaa
     3841 ttagaaaaat ccactaatcc tgagaatgct ttgttattct catttattac tgttgttttt
     3901 tcattatttg ctaatttatt tttcagttca ttaaatgtta aatataactc tataaattta
     3961 attttacagt taatatgtat tatagttctt atatttatat atattaatac atttttatat
     4021 cgcctcttca atttgagccc catcattata taaaattaac ctacttgata tcttaattta
     4081 atatatgtac actattcata tcttaatagt caatattatt catttttaat agctcttttt
     4141 agatgtcaga attattatat tttgaaattt attaaatttt tgcattaaaa aaactagaaa
     4201 ttaatttaag tcttgaatta cttataactt tctacgtatc tacctttata ttcttactct
     4261 gattttatat ctaatattaa ctcttcatca aagctattaa atatacctct aattgagtca
     4321 attatttcac ttacttcacc tttaattttt atattaactt caaccatttc aaatctcatc
     4381 tttttatctt ataatttcta taagtttttt tatttattgt gttgcttact ttgaccagca
     4441 cttttttatt taatagtcta aatgatattt tttaccagtt ttaatatcct taaatactat
     4501 atttactaaa aacttataat ttcttttaat acattcttat tactaataac aatactcaac
     4561 aattttatcg ccttttttca aatatgattt aactttttta ttcctaatta tttaaaaata
     4621 gttgtccaat tttcttctat gagaaagtgc taagtattag caacttctct attctcttaa
     4681 ctttctactg tctcaacttt cttcatagct atattaatag ctacttcttt agctacttca
     4741 ttgaactctt cccatcttct cctattttct tcatcagata tacgtggata tactatataa
     4801 acttctgtgt tttgattagc tataattttt tcatcatatt ctatattttt ttctttatca
     4861 tatttgtatg gtttaaattg ttgtagtatt ttcataacat caccccttgt tataaaatat
     4921 gaactcataa acttgtctta tacttattta attgttacaa tttttactta aaatcacttt
     4981 atattctata ttttaaagct aaatccttta caaaagtaac atattcttct ataagtttct
     5041 tatcctctta tatagtatct agtttgttta acttatctct tttaaactta catacgcctt
     5101 cttctgccat tcgtctttta ttaattgtta acattgtttc aagagacaca ccaaaactct
     5161 tgttaagaag ctcttagctc tcattagtaa aatattttac ataacttaaa tcaccttgac
     5221 ctctagcaat tttaactgtt aatagacttg tttctttttt ccaatgtatt atatttaata
     5281 taacaacatc ttttattatt ttaacttcta tttttacatc taatgccata ttattgcttt
     5341 attaatttgt atccttaaat cttaaatttt ctattaatac atcttctata cagcttagtt
     5401 tctctcgctt aatcttgaaa tatattttct ctaaattgtc aaattgttcc caagttttat
     5461 caatatctaa tattttgcag tgtctatttg cacatctttc gatccaaagg tataattgac
     5521 tagaatgttt tgaaatcaac tgactattag taggttagtt cttaaattct ttcaaatact
     5581 cgaattgtaa taaaacataa tttacattct ctttatttct attgaagtta gtttatatat
     5641 tgtttacatc attttcatat atatctgcta gttgctatgc atttaaaatt ctcttattat
     5701 ttctttatat tacttgtaaa ttattcatcc tcatcatcct cttaattaaa tttctatctt
     5761 agaaatttaa tttgtaaaaa aattttctat atcataatca agtatcttag atagtttata
     5821 ggtaacatat caactctaat tgaatattac cccttcctgt acttaaaata agttgatgca
     5881 tttttaaaac ctaactcttt tgcaacactt tgtaagtata aattttatgt atgtcttctt
     5941 ttttataaaa tcatagtgta tctttttcat atattcacct taattgctat atttcaataa
     6001 caatatttcc aaaacagaaa tttttcgagg taattatgta tagatgtaaa tcaaagaatt
     6061 aaatatacta gggaaaatat gaatagttct caaaaagaat tgcttgaaaa aacaaatata
     6121 aatacaagtg ttatgaatag aatctggtga aagaccaaca agagacgatg ggttaataat
     6181 atttgctaaa atttttggtt tatatactga ttatgttctt ggtttatcag atacagaaaa
     6241 actaaatatt ggcaattaaa actgaattga ttgtattata tgtgtgatga atataaatat
     6301 agaatttttt gagaagcttg aaatagctta gaaaaaataa gagattcact caagaataag
     6361 ttactaaaga ttaaaaaata acaaaagtat ctactaaagg atatgaaaat ggtttaagag
     6421 agttaagtct ggaaatattt atgtgtattc tttaaataaa aatttatttt ttaaatgata
     6481 attaaatttt tactgatatg gctatgcttt taaaagtcat aaccttattt tgatattaga
     6541 tctgtcttta cataccactt agttaatata aaactaaata tagcttgtaa ctcctttgat
     6601 gcacttaaaa gctttacata ccacttagtt aatataaaac gaatttagct atagaagcat
     6661 caaattgtgg cacagcactt tacataccac ttagttaata taaaaccttt aaagacaaaa
     6721 cgtcgtataa ttaaatcata gcaactttac ataccactta gttaatataa aaccttataa
     6781 ttctttaagt actagactga gcaatttaat ctttacatac cacttagtta atataaaacc
     6841 atcccattca gcaacaacag tcgctaaatc ttctgccttt acataccact tagttaatat
     6901 aaaactttta gtatattttt taggtctgcc ctctttaaaa ctctttacat accacttagt
     6961 taatataaaa cacgaggtta tagttaattt tgtcgtggga gtttataact ttacatacca
     7021 cttagttaat ataaaacaat ttatatatat atatatatta aagtcagacc cctttacata
     7081 ccacttagtt aatataaaac ttgttgttca gcattagcaa tcatatcatc tattcctttc
     7141 tttacatacc acttagttaa tataaaacct gtaccatcag agacagtcat taaaagattt
     7201 ataaacttta cataccactt agttaatata aaacttataa attgtctctg tacccatttt
     7261 attcaatgtc gtactttaca taccacttag ttaatataaa accatgaaaa aatgggtaat
     7321 tcaaactctt ttattttctt tacataccac ttagttaata taaaacgttg gagaaaataa
     7381 tccttcttat gatttgccaa gcggctttac ataccactta gttaatataa aaccagcgga
     7441 tgaattattt atgttaattg gagatggaga ctttacatac cacttagtta atataaaacc
     7501 ccaaaataaa ttgagtattt ccaacactta catctataca attctctcaa atttgcagtg
     7561 aaccatgagt agtgcaaatg ataacattta tcacataccc tcaatgcctt atatttcaac
     7621 tgttaggccc tattttatca aaaatatcgc tcactgcaaa actatttata attttataat
     7681 attatgaata tattattttt aaatatctgt accaatttgt agtataataa aatcaagaaa
     7741 ataatttact cctataaaga gtgattattt ttaagtgaaa atattatttt tttaaatcat
     7801 ccttattggc gtttgggaga tttttatttt atcataaata taaactgata taacaccagc
     7861 tgacatgctt gctgctttcg attattattc ttgctacaat tattataaca tataaaactt
     7921 acttattttt accaaatatt ttatacaaac aataaatatt aacttatgaa atatctactc
     7981 atttacagat atttcataag ttaatagctc tataataaaa tttttatcat tgtttataaa
     8041 aaattttact ttgcattttt ttataaacaa cagttttatt tataatgttt tgtggtataa
     8101 taaaagcaag aagaactaca atctattttg cgttagagtg gagttcatca taaacagagt
     8161 ttattttttg gatttaaata taaatttaaa ttcaactcgt aagtcactct tt
//
LOCUS       DAGQWR010000166         8200 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.168, whole genome shotgun sequence.
ACCESSION   DAGQWR010000166 DAGQWR010000000
VERSION     DAGQWR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.168"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..552)
                     /locus_tag="MIW95_003374"
     CDS             complement(<1..552)
                     /locus_tag="MIW95_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY3152671.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEISTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDIL"
     gene            complement(799..1293)
                     /locus_tag="MIW95_003375"
     CDS             complement(799..1293)
                     /locus_tag="MIW95_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBY3152672.1"
                     /translation="MSTLVTLLGLLVCTKISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKLSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPRMPLSVSLALAAVISSTDLVAVKSITQGLNFPKNMMSILEGESLLNDDDRII
                     NIME"
     gene            complement(1445..2416)
                     /locus_tag="MIW95_003376"
     CDS             complement(1445..2416)
                     /locus_tag="MIW95_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBY3152673.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPDEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTRALMSAVPVQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(2417..3394)
                     /locus_tag="MIW95_003377"
     CDS             complement(2417..3394)
                     /locus_tag="MIW95_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152674.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMVIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPVLGKNKDKRLYSIPGQV
                     PNPIGIPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            3613..5172
                     /locus_tag="MIW95_003378"
     CDS             3613..5172
                     /locus_tag="MIW95_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY3152675.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKRKEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            5278..6237
                     /locus_tag="MIW95_003379"
     CDS             5278..6237
                     /locus_tag="MIW95_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152676.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            6240..7052
                     /locus_tag="MIW95_003380"
     CDS             6240..7052
                     /locus_tag="MIW95_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152677.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVISLTIISKKFSSNT"
     gene            complement(7267..8169)
                     /locus_tag="MIW95_003381"
     CDS             complement(7267..8169)
                     /locus_tag="MIW95_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY3152678.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
BASE COUNT         2856 a         1229 c         1050 g         3065 t
ORIGIN      
        1 tagtatgtct tttacaattt tagtaattaa cagtgattcc tttgaacttc ccttatagtt
       61 tgaatttact atatgcattg ctataaaacc agcttcatct actggaaatt tcacatcaaa
      121 ttctttattt atataatcta cagcccattt accaatctca tattcttttt tatgtattct
      181 ttttatctca tgtagtaaat cattctttat agttatattt tcatgatatc ttttaattgc
      241 aaatgcaata tggtcagtaa gtgctacata aatatattca taaagcttag tattcaaatt
      301 ctcctctgca taagcaatta tttcagtact aatctcaaaa aatttctcat caacctggct
      361 tgctagtttg ctaattttat ctaaaaagct ttcatcttgt attacaaaag ttctttcaat
      421 tttactttta tctatttctt gacctttctt tttttgaaac ccaatcccac atcctgttag
      481 tattaattct ttttttatat tttcatctaa aactaataca acattattat tataaatttg
      541 ttgaataatc ataatcaata tctctcccaa aatacaattt cccttattca taaaaaaata
      601 cctaaattaa gatacaattc acctgtatac cttaacctag gtattgcctg cttaccagta
      661 acaatccagc ttgttaattc ttatatattg tattcaattt atggttttat tctattataa
      721 ataaatatat atgtcaagta aattattgaa aactatttcc tattttttag tattattttt
      781 aacatcaaaa atcaaatttt attccattat atttatgatt ctatcatcat catttaataa
      841 agattcacct tcaagtatac tcatcatatt tttaggaaaa tttaatcctt gtgtgattga
      901 ttttactgct actaaatcag ttgaagatat tactgctgcc aaagcaagtg aaacagataa
      961 tggcatccta ggaataagaa aatgtataaa gattccacct gcaaacacgg ttatcaaaac
     1021 taaaccaaat gcaagaagca aaattggttt tctaagctct agtaactctt ttcttgatac
     1081 attttgtcct tcactgaata ataatggagc tatgatacat attacaaata tttctggatt
     1141 aaatgaaaat gataatttaa acggcaaaat agacaatata atccctagta caatttgata
     1201 tattgcaaga ggaatattac tccacttttt gctaaataca gttgaaatct ttgtacaaac
     1261 caataatccc aacaatgtta ctaaagttga cataactacc tctcttttca ataataatat
     1321 tataaattaa acttatatat caactaactt aatattaaca acatgaaatc ccatcttaat
     1381 caaaaaaatt ttcagtttat tctattattc ttattatata catcaaattt aatttttgaa
     1441 atatctatac tagatggcat gcaacaaaat gatttcttga tatttctttt aaaactggaa
     1501 tctctttttt gcatatttcc ttacaataag ggcatcttgt atgaaactta catccacttg
     1561 gtggatttgt tggagatggt atgtctcctt ttaatataat tctatttttc ttaattgtag
     1621 ggtcttgtac tggtactgcg ctcataagtg ctcttgtata tggatgttga gaatgtaagt
     1681 aaagttctcc tttagttgca atttctatta tagaacctag atacataaca caaactctat
     1741 gtgcaatatg atttactaca ctcaaatcat gagatataaa taaatatgag agtttatatt
     1801 tatcctgtaa atccataagt aaatttataa tttgagattg aatagaaaca tctagtgcag
     1861 aaacaggttc atcacaaact ataaattctg gatttaatgc taatgctctt gctataccta
     1921 tcctctgtct ttgacctcca gaaaactcat ctggttttct atttaaatgg tacttagaaa
     1981 gaccacaact ttctacaaca tttaatatat tatcttttat ttcattcttt ggcactaaat
     2041 tatgttctat aactgcctct gatattattt gttccacatt taatcttgga tttaatgaac
     2101 tatatgggtc ttgaaatatc atttgcattt tgggtcttaa ctttctaagt tcttccttct
     2161 ctaacttata tatatctaca ccattaaatt caactttacc ttcagttttt tcaattaatc
     2221 taaggatggt tcttcctgca gttgattttc cacatcccga ttctccaact atagccaaag
     2281 tttctccttt ttttaaatca aaagaaattc catctactgc ctttacacat ttaatttcct
     2341 tatcaaataa tccagattta acttcaaagt actttttaag accttctact tttaataaac
     2401 tctcttcttt tttcaattat atctcctcct ttttatataa ccaacatgca atactatgac
     2461 cactattcaa ttctattaaa ggaggtatct gagttctaca tttatcacat actttttcac
     2521 atctatcact aaaataacat gaatctggta ttccaattgg atttggtact tgtcctggaa
     2581 tcgaatacaa tcttttatct ttgttttttc caagaacagg ctttgatttt agaagtccaa
     2641 tagtataagg atgttttgga tttttaaata attcaacaac ctctgccact tctataattt
     2701 taccagcata catcactaat actttgtcac acatttctgc aactactccc aaatcatgtg
     2761 tgatcatcat tatagatgtg tttaattttt cttttatatt tttcattata tctaaaatct
     2821 gagcttgaat agtaacatct aaagctgttg ttggttcatc agctataagt aattttggat
     2881 tacatgataa agccatagct atcattattc gttgtctcat accacctgat aattcatgtg
     2941 gatatgaatt atatatttct tcagctcttg gtatttctac cagttttatc atatcgatta
     3001 cctttttctt agcttcttcc tttgataaat cttgatgtat cataatacct tcaatgattt
     3061 gacttccaac agtgaacgct gggtttaaag atgtcattgg ttcttgaaaa atcattgata
     3121 tctcattccc tcttattacc ataagttcat cttcattcat ctttaaaatg tctttgcctc
     3181 gaaagattat ttctccacct tctatttttg atgttggaag taacttcatc aaagacatgg
     3241 ctgttacaga cttaccacat cctgattctc caactattcc aatagtttca ccttcattta
     3301 tattaaaact tacttcattt acactcttta caatcccttt atctgtatga aaataaactt
     3361 ttaaatcttt aacctcaatt agtgctttac tcattatttt ctcctctcag caaaattact
     3421 taaaataaca tacacctttt gattgtattt tataaaaatt taaattacaa cattttctta
     3481 cattttatta tttttttcta ttaattataa taatataata gttaatttct atatatattg
     3541 atttaataat gattactatt atacaatgaa aaatatgtgt taaatattat tttatggagg
     3601 ttttataaga atatgaaatt taaaaaattg gcatcattaa tcttagtttc atcattaatg
     3661 cttacattta cagcctgtgc ttccaatgat aaagacaagt ctagtggaag caaatctggt
     3721 ggaactgttg taatacctat ggcaagtgac ccagatataa tcaatggagc ttttgcaaac
     3781 gttaaagaag actctcttgc atcaaatata gtttatgctc ctctttatac ttatgaaaaa
     3841 gggaatttag taaattattt agcagaaaaa gtagatttta aagatagtaa ggaattaact
     3901 ataaaattaa aatccaattt aaaatggcat gatggaaaac caataactgc tgaagatgtc
     3961 ttatttacat ttaatacagt tttagacgaa aaacaaaatt caccatcaag acaatactta
     4021 ttggtgggtg aaaaaccagt taaagtagaa aaaattgatg atttaacagt taagattact
     4081 ttaccaactg caagtgaatc tttcttatat ggtatatcta aaatttctcc aataccaaaa
     4141 catgtatttg aaggagaatc aaatatagct aagtcagaaa aaaataataa tccagttggt
     4201 tctggtgcat tcaagtttaa ggaatggaaa aaaggtgaaa gcatagtatt tgaaaagaat
     4261 gcagattatt ttggtggaga acctaaggca gattctatag cgctaaaaat aattcctaat
     4321 gaagcatctc aagaagcagc tttaaataat ggcgaaattt cacttatgaa gacatctgct
     4381 gaaggatatg aaaaggcaaa atcaaactca aacttacaaa cttatactta tagtgaagaa
     4441 agattaaact acattgtatt taaccaaaat atatcaaaca tggctaacaa agaagtaaga
     4501 caagccttat catatgcctt aaatagaaat gaaatgatag aatcagcata tggaaaagaa
     4561 ggttctgtac ctgctaaatc tatattagtt ccagaagcag acttctatac agaagaaggt
     4621 gttgaaggat atgaccaaga tacaaataag gctaaagact tattggataa aagtggtgtt
     4681 aaaattgata aattaaaaat aggttataac acaggtagat ttgggcataa aaactatgcc
     4741 ttagtagctc aacaagagtt gaaaaaaatt ggtatagaag ctgaaatagt tccttatgaa
     4801 agtaaggcat tctttaatat actatttagt aatagcactg aatgtgatat gtatgtaaat
     4861 ggttatgctt ggggattaga accaaaccca tatagaggta tgtttgaaac tggtcaatat
     4921 tgcaaccaaa ctaaatattc taatgctgaa atagatgctt tatgggaaaa aggatttact
     4981 gagttaaaca aggaaaaacg taaagaaata tacaaacaaa tacaacagga tatatctaag
     5041 gatgcaccta tttacactat agattatgaa caaaatttaa tggcagctca aaaaaatcta
     5101 aaaggaataa aagatgctaa accatctcct gcaatattat ttgaagattg gtctaaactg
     5161 tatgtagaat aattgatgta aaattttata ttattaatta gaaaaggaga tgtctaaaaa
     5221 taatattgtt ttattatttt aaaacgagac atctctttta attaggagaa tttacttatg
     5281 ttaaaattaa tattgaatag agtaaaagtt ttaataccta tgttaatctt aatatctata
     5341 ttatcatttg gacttttaga attagctcct ggtgacccag cagatgcata tataaatcct
     5401 ttaatgtctt cacaggatat tgaaaatata agggttaata tggggcttga taaaccagta
     5461 tatataagat atttaaactg gttgaaaaac actttaagtg gaaacttagg tatttcttat
     5521 ataaaccata tgcccgttac agaacaaatt atggaaaaaa tgggaaatac atttattctt
     5581 atgggtactt ctttgatatt ttcaatattg gtagctatac ctcttggaat atttttagct
     5641 gtaaataaaa attcaataac tagtaaagta tcatctgttt ttaattatat aggtgtttct
     5701 atcccttctt tttggatagg tatgatactc atttcaattt tttcagttaa acttaacatt
     5761 ttcccatcag gaggtatgca tactattgga aatgatagta ttggagattt agtaaaacat
     5821 ttagttttgc ctgtaataac tttaggtcta tataatactg ctatttttac aaattatgta
     5881 gaagctagtg taaatgaaca attaaaaaaa caatatgtta ttacagcaag agcaaaagga
     5941 ttatcagaaa aagtcatact atttaaacat gtcttgaaaa attcactgac atcattggtt
     6001 acaatcctag gcatgagtat acaaaaatta gtcactggtg catttgttac agaagtagtt
     6061 ttctcttggc caggtatggg aagattaatg attgattcaa ttttttcaag agattatact
     6121 gtaattatgg caataactat gttatcagca ctatttttaa ttttaggtaa tttagttgct
     6181 gatatactat acttattaat tgaccctaaa ataaaatcta gcaaaggagg attctagttg
     6241 tgtttacaag tatcttaaaa aaattaaaaa aagataaatc ctttgctttt gcatgtatta
     6301 ctcttagcat aataatcata ttaattatag cttcttttat attaaatata gactctaatg
     6361 caattgatac tacttctaaa cttcaagaac ctagcttaaa gcatatcttt ggtacagacg
     6421 aacttggtag agattacttc tctagagctc tttatggtgg aaggatttca cttagtgttg
     6481 gtattttatc aatgcttatc tctgtagtat ttggtactgc tgtaggtgtt attagtggat
     6541 atattggagg taagctagac caattcttga tgagattagt agatatgctt atgagtatac
     6601 caagtttctt agttttgatt gtattaaata cttatctaac acctaaagtt tccactatta
     6661 tactaattat tggattctta agttggatgg aagtttcaag aattgtacgt ggcgaaacat
     6721 taaaaataaa ggaaaatgaa tactgtttag ctgctaaagc tactggtatc aagactagaa
     6781 atatactatt aagacatata gttttaaact taaaagagac tattgtagtc gctggttcgt
     6841 taaatattgc taatgctatc ttaactgaat ccgctcttag ttttttaggt cttggaatcc
     6901 aattacctct tgcatcatgg ggtaatatgc ttcaagatgc tcaaagacat atttttgaca
     6961 agttatattt agctgtattt ccaggattac ttatattttt aacagtcata agtttgacta
     7021 taataagcaa aaaattttct agtaatactt aacaaatttt atatagaaaa taaaaacagt
     7081 gattatctaa agatatacac tgtttttatt tttcgtttaa aaaatacata ttttgtatat
     7141 tataacttta aatactactc taccatgtta tgtaaagcct attattgctc tcaaacatat
     7201 agatgaaaaa ctaatagttt attgtaataa aaattgattt atttatttgt tattttaata
     7261 acatttttaa gagttctttt tactaaagaa atctataata aatttaaata catgttggtc
     7321 tacatggcta taacgttctt ttttaggtct acatagtata actttccatg taataggatg
     7381 attaaaaggc ttttctataa ttttgctatt ttcaaaaaaa tcatgaactg gtgatggtaa
     7441 aattgttata aaatcagaat ttgtggttga tagcaacaaa aaatcccatg aaccagacat
     7501 tatatatctt tttaacttaa cattttcatc agtaaatttt tgcattagct tatgatgtat
     7561 cataaaagta ggattaaata ttgcaagtaa ttgctcattc aaatcgttcc aatctatctt
     7621 atccttctta gcaagtggat gatttatatt taaaaatgct gtaagttcat cttcttgaag
     7681 cacatgttca gttactacac ttttatcaat tttatgtgga tgtaacagta ctgcataatc
     7741 tagttcctgc aagataagca ttctatttaa gtcatatgca cccttctcta ctatatcaaa
     7801 ttctatatct ggattatttg caactaactg tgcaactaca tctgaaaatg ctattccaag
     7861 tataagtgga ggtattccta ttctaacttt tcctttaaat tgaactgaat cttctcttaa
     7921 atcttccatc atatttttgt attcatttat aatatgttct gcatttacat aaaatctttc
     7981 tcctgctggt gttaacccat ttaacctacc tttatacctt tcaaataaat atacatcttc
     8041 actttcttca aaatttttta taatttgact taatgctggt tgagatatgt gaagtgtctt
     8101 agatgctatt gataaattaa aaccagattt cactatttca acaaaatatt tcaactgttt
     8161 tatatccatt ttaaaccccc ttccccaaaa gtataattta
//
LOCUS       DAGQWR010000167         8192 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.169, whole genome shotgun sequence.
ACCESSION   DAGQWR010000167 DAGQWR010000000
VERSION     DAGQWR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.169"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..408
                     /locus_tag="MIW95_003382"
     CDS             <1..408
                     /locus_tag="MIW95_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY3152679.1"
                     /translation="FGGKIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFFYD
                     LVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVTCK
                     LYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            873..2087
                     /locus_tag="MIW95_003383"
     CDS             873..2087
                     /locus_tag="MIW95_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3152680.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEKVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNIFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLWYANLSLEIKKMLSEDKGAFFSMYVDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(2507..3028)
                     /locus_tag="MIW95_003384"
     CDS             complement(2507..3028)
                     /locus_tag="MIW95_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3152681.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSYLQTEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            3384..4181
                     /locus_tag="MIW95_003385"
     CDS             3384..4181
                     /locus_tag="MIW95_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBY3152682.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            4291..4881
                     /locus_tag="MIW95_003386"
     CDS             4291..4881
                     /locus_tag="MIW95_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBY3152683.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNEETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFADFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            4947..6644
                     /locus_tag="MIW95_003387"
     CDS             4947..6644
                     /locus_tag="MIW95_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBY3152684.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGISLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     NVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            6858..7697
                     /locus_tag="MIW95_003388"
     CDS             6858..7697
                     /locus_tag="MIW95_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBY3152685.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         3287 a          780 c         1345 g         2780 t
ORIGIN      
        1 tttggtggca aaataataag aagaaatgaa aagactaagt ttgactggtt aagtagagat
       61 aaagaacaag ttgaaaaata tataaatgac ccattttgtg gagtagtgtg tagttgtggc
      121 tttttttatg atttagttca agggttaaaa gaaattgagg ataaagaaaa cttaaaaaaa
      181 gtgccacttg atatacctat ttacataata tctggagata aagaccccat aggaaaaaat
      241 ggtaaaggag tcttaagact aagggataga tatataaaat taggagtaaa agatgtaact
      301 tgcaaacttt acaaagatgg aaggcatgaa ttgttgaatg aaataaatag agaagaagtt
      361 tttgaggata taatttgttg gttaaacaat aaaatagaaa tattgtaata aaaaaatata
      421 atgatatatt tgctttaaat ttatttatat ataatatttg aaaagtggat tatgtatatg
      481 caaagtagaa aagagagagc ctagaagtga ttttttgcta taatgccatt tattgaatag
      541 gtgggtgtta ttataaataa attattaaca ctcagtatta ttatcattgt atatggaatt
      601 tattttattt aaaattcctt gaaagtttaa atatagtaag ttgtagtaaa gagaaaatct
      661 aaatatttaa atgctattta atgtgaatct attaattagg ttatacttaa acttaattaa
      721 tagattttat atttcaagcc aatgagatag ggttttagat taaaatataa ccaatggtta
      781 aattacagac attttgttaa ctttccctcg atttactagt aaatattttt tttatatact
      841 taaattacaa cagatgtact agggaggtat atatgcaaat taaaataggt aaagtcatac
      901 agcgtcttag aaaagaacga aatttaactc aagaacaatt agcaaagttt ataggtgtgt
      961 caacaccagc agtatctaag tgggagagtg gaaattctta cccagatata gaattattgc
     1021 ctttattagc agactttttt aatgtgtcta ttgataagct tttaaattac aagattgatt
     1081 taagtgaaga aaaagtaatg aaaatttata aagaattaga atcaggcttt gctagaattg
     1141 aaattgattt atcaacagaa gaaccaaaag aagaatttag acaagactta gaatctgtaa
     1201 aaaagctttc taatatgtat atagagaagt atcctaaaag ctatttattg aagctaagga
     1261 tatgttcgct gtatcagatg tattcatata aatttggtaa gagtgaactt aatgacagag
     1321 taaaagaaac aacgaatatt cttgaagata tagtgagaaa tacagatgat atccaaataa
     1381 aagaaacagc tcttattata ttatcaaacg catattgtat gttggaagat tatgaaaagg
     1441 cagagttgta tttaaatatg atacataagt caatagggga tacaagtgtt aatttagcta
     1501 tgatatattt aaagcaaaat agacttgaag aagcagaaat attgcttcaa aataaattgt
     1561 ttagtaatat atttaatata agcatggatt gtaagggaat aattaatgta tacaaaaatc
     1621 aatacaagga attaaagaaa aaattggaga atagaaattt taataaaaat gcaattgaga
     1681 gagaaatgga gtatataaaa aataagttgc tgtggtatgc aaatctttct ttagaaataa
     1741 aaaaaatgct cagtgaagat aaaggtgcat tttttagtat gtatgtggat tatatggaat
     1801 tatctctaat ttttttattt tttaacatga aagaagaagc aaaaaaagcc ttatatagtc
     1861 taaaagaaat attagaaaaa tatcctatac atgaaagttt ggatgtaagt caaatgagat
     1921 tttttgataa ggtagagtct aaaaatttat atacttttaa tatatataca aacttgttaa
     1981 ttgtacttaa tgatgacagt tataatgagc ttagagaaga accaattttt aaggatgtaa
     2041 ttgaaaagat tttagatatg gaaaaaatgt tgaaaaataa agaataagta aaaatttaat
     2101 ataggactta aagattaatc aaattaaaag aaagtgtttc aaaataaaaa tttgaatcac
     2161 cttttttgtt ggtcttaaac cgttatttag aaaaatagag gtttcaaaat agaaatctga
     2221 attatctgta ttattacaca aaaatagaaa cctaaattta taaaaataga ataaagtttg
     2281 ttatataaaa tgtaattttg tctataaaat ctcacatgtt ttcttaactt taagcgtgaa
     2341 aaagaggatg tatcatgaat tttttagttc atgatgcatc cttatttgta tgaaatccaa
     2401 tatatcttta ttatttaaac aatggaaaag agtcttattt ctattttgag acatctcttt
     2461 tatttaatta tgaagtttta tacagttcaa taaataggaa ttatatttag aaactcatga
     2521 cattatttct tagatagtga ttaaatttat catattccat tttagataac ttttctgggt
     2581 aagacattac aatcaggtta tgcccattga atttttcatg gtatggtgga tgaatgtgtt
     2641 cataattatt tgtcttatat tttactcttt catagaacgc cttgcgataa attgaaatct
     2701 catcaattgg aaggtcaatt tctaaaataa tagtttttcc tttcttattt aaaagctcta
     2761 acgctctttg tccatatcta ttattgcgca tttctggaaa aatgcagaaa tgttctacat
     2821 agataaagtt atctgtttcc caacataaaa ttatgccgac aaactggttt ttatcataaa
     2881 ttaaattgaa ttgatattct ttatttttca aaatttctgt ttgcaaataa gattttcttt
     2941 gttcatgaaa tgggaaacta attttatata attccatagc cttgtaatat aatttatttt
     3001 cactggttct taaccgttca aatttcactt tactcctcct aatcacgaaa attccaattt
     3061 atttatgagt tcattttacc atagtcagta tttgcataca atgtgtttta agttgagtgt
     3121 catttctttc ttttatttat gcttatatct catagactaa gagataattg cttaagacat
     3181 aactaaaaac tattatatta agctttgata atctaaaaac agtatattta tggacttttc
     3241 taggtctaaa agtaatctta ataattttag aagttacaaa aaagttacat aaatttggaa
     3301 agctataata tacatatata atataaggtt aaaactgtaa tgaataatat taatttaaaa
     3361 tttgaacatg gaaggagatt attatggaga aaagagaaaa gaggataata atatcatctt
     3421 tattaagtgt atcaatttta atgggtttgg taagtatata ttctatatta aataaagaag
     3481 acataatttt aactgttaag ggtcaagagc aaaaagtatc ttcctttaag aaaacagttg
     3541 aggaactttt ggatgaacag ggtgtaaagt ataattctga agataagatt aatccaagtt
     3601 tagatacaga actaaaagat gatatgaaaa taaaagttgt taaggtaact aaaagtaaaa
     3661 aagaagagat tgaaaaaatt ccatttgata caaagcatgt aaatgatagt aatttgttaa
     3721 aaggaaaatc taaagtttat caagaaggtc aagaaggaga aaaaaaacta gtctataatt
     3781 taacttacca tgatggaaaa ttagtcaaaa aagtcttatc aaaagaagta atatctaagg
     3841 agccaactac aaaaattata aaatatggaa ctaaagaaaa agtactaata gcatcaagag
     3901 gagcaaatat aagaggaggc aaacatatga aagtggttgc cactgcatat gcaggagata
     3961 caataacatc tactggtaca actccaagat ggggtgttat tgcagttgac ccacgtgtaa
     4021 taccatatgg aacaaaagta tatataccta aactgggtat gacttttgta gcagaagatt
     4081 gtggaggtgc aattaaaggt aatagaatag acatttttat gaacagtgaa ggaaaagctt
     4141 caaattgggg aaggaaaagt atagatatat atctacatta aattagtgaa tagaataact
     4201 ttacttgaag gacaaaaaag tataaaatat gaattaacta aactgttaga ttattcttaa
     4261 aacataaaaa tatatctaag gggggataag atgaaaatag tctgcttagg agacagttta
     4321 acgtatggat ttggagtatc acgaagtaat tcatggacaa acattgtaaa tgaagagact
     4381 cgattagaaa tagtaaataa gggtataaat ggtgatacaa caagtggtat gttagctagg
     4441 tttaatgaag atgttgtaaa aaactctcct gatatagttt ttataatggg tggcactaat
     4501 gattttattg ctggtgcggg aaatgaaatt attaattcta atataatggc tatggtgcat
     4561 cagtcatatt caaaaaatat tatacctata ataggtatac cattaaaacc agatattcca
     4621 aatgttaggg aagattggag ttgttttact gattttaata tagtatctca aaacttagaa
     4681 tcatatagtt attggataaa aaaattttgt atgacattta atactaattt cgcagatttt
     4741 tattcagaat ataataaaaa tatggaacta gaaggatata aaaaattata ttttgatggg
     4801 ttacatccaa ccaaagaggg tcatagaata atggcagaca tttttattaa ttcaattaat
     4861 aagtatatag aagagttata gattacatta aaatgcttgt ttatgcgtca gttgcaataa
     4921 atacatacag tagagagggg tttattatgg attatactaa aacttatgaa gagtggataa
     4981 agggttcata ttttgatgaa gatacaaagc tagagttgga gaatataaaa aataatgaaa
     5041 aagaaataga ggatagattc tataaggatt tagaatttgg gactgcgggt cttagaggaa
     5101 taattgaagc aggaacgaat agaataaata agtacacagt tagaagagct acttttggat
     5161 tggcaaacta tatattagaa aacacaacaa aagaagaaac aagtagagga gtagtaatag
     5221 ctcatgataa tagacacaag tctagacaat tctgtataga atctgcaaat actttggcag
     5281 catgtggtat aaaagcatat atatttgata gtttgagaac tactccagaa ttatcttttg
     5341 ctgttagaag cttaaatgca atcgctggta tagtcataac agcaagccat aatcctccag
     5401 aatataatgg atataaggta tactgggaag atggagcaca agttatgcca gagattgcaa
     5461 atgcaataac agagaaagta aatagtatac atgattatag tacaatacca accttaacgg
     5521 aagctaataa aaatttggta gttttgttgg acgaatcaca agatactaaa tttatagaag
     5581 cagtaaaaag ccaaattata agaaaagatt tagttaaaaa tgtaggtaaa agctttaaga
     5641 tagtttatac acctctttgt ggaacaggga atgttccaat aagaagagca ttgaaagaag
     5701 ttggttttga aaatataatt gtagttccag aagaagaaaa tccagaccca aattttgcag
     5761 ggcttgatta tccaaatcca gaagagaaaa aagctttaaa tagaggtata ttactcgcaa
     5821 aagaaaaagg agcagacctt gtaatagcaa ctgacccaga ttgtgataga gtaggtgtag
     5881 cagtaaaaac tactacaggt gagtatgctt tacttacagg aaatcaaatt ggtggtatgc
     5941 tgacacatta tataatagaa ggattaaaag aaaataacaa attaaaagaa aatcctacaa
     6001 taataaaaac catagttaca tcagaatttg gagcagatat agctaaagca aataatgttg
     6061 atgtattaaa tgttcttaca ggttttaaat ttattggtga aaaaataaaa ttatttgaac
     6121 aaaataaaaa tagaagttat gtatttggat atgaagaaag ttatggttac ttagttggga
     6181 ctcatgctag agataaagat ggtgtagttt cttcattgtt aatatctgaa atggcagcat
     6241 tttactattc aaaaggtata agtttatatg atggtttaat agaactttat aaaaagtatg
     6301 gatttttcaa agaacagact atatctttga ctttaaaagg tatagaaggt gtagaaaaga
     6361 taaaagaaat aatatcttat tttagagaaa atcaaattga ttatataaat agtattaaag
     6421 tagttgataa aaaagattat aaaaatggta tagataatct tcctaaatct aatgttttaa
     6481 aatacttttt ggaagatgaa tcttgggtag ctataagacc atctggaaca gaaccaaaac
     6541 ttaaatttta catagctgta aaaggagcta gtgatattga agcagataaa aagattcaag
     6601 gtttgaaaaa gtatatagat gacatggtcg aaaaattaaa ataaaatatt attattttat
     6661 aagataattt tgtaataata tttatttagc tgaaaattat aatttacttt cattgagtag
     6721 aatatggaaa tgttagtaga attgttattt tcagctttta tttgtgttat ttttacttaa
     6781 ataattatat tatagaatga atctgagtat aattaatatt gtataatgaa attaatttga
     6841 tagaaaaggg gatatttatg tttggtatag ataagaaatt gattgaatat ataattgcaa
     6901 aatccaatta cagtgaatta agttcaaagt cagcagaagt atctttcttc ttaatgttgt
     6961 ccatatttcc ttttttaata tttacaataa gttctatagc ttatataccg attcttcatt
     7021 taaataagta tatagctttg tttagaaata tgatgccaga aggtgctttt gctgtgttat
     7081 cttcaatcat agtttctgca atagacaata gaaacctgaa atttttagca gtcagttttg
     7141 tacttactat gtggacattt tcaagagcag taaaagctct tattaaagga atgaatagag
     7201 cctataaagt taaagaaact aggtcttttt ttaaaatttt atctatatca tttttattta
     7261 caataatgct tttggtcctt atttttttat ctatgatatt tttagtttac ggggaaaaaa
     7321 taggttattt tatttttaat cttgtaggat tggatgagat atttataaaa atatgggata
     7381 ttctaagata cactgtaggt ataataacaa taatagtgat atttacactt ttatataaat
     7441 acactccaaa taaaaagtta actattaaag aatctgcgcc aggtgctatt tttgctactt
     7501 ttgcttggtt tttagtatca tttttatatt cttattatac aaattactat gctaactatg
     7561 aagtaatcta tggaagtata gctgaaataa ttgtacttat gacctggatg tattttagta
     7621 gttggtctat cgttattggc tatgaggtaa attcaagatt gtattttaga aagataagac
     7681 atgaaatgtt aaagtaaagt atttaaaata aaagagagta tttagtatta taaatgtaat
     7741 tgaattaata aagaaatatt gacatttaag ttaaaaagtc tagaattttt ctgggctttt
     7801 tttattttta gtataacttg acaaatttta tttaatggga tagttttaat ttataatcat
     7861 ctttgaaaag atgactattg ataagtaagg tataaaattg gtaagtatca aaaaatatac
     7921 taaataaaaa ttatagtcct aatgagttaa tttctgtatt ttcaaataat gcagtattta
     7981 gtcatgtgtt tttatatgcg cctgtatttc caattaccaa tatattagaa taagctactg
     8041 taattagatt tagaaaaaag cgacataaat aattataaac aaataataaa ttacataaaa
     8101 ttaaatttat tcacaagtat atcttataaa atatgttata attaatagaa aattgtcata
     8161 taattaatta agtagtatta ttataaataa ca
//
LOCUS       DAGQWR010000168         8174 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.170, whole genome shotgun sequence.
ACCESSION   DAGQWR010000168 DAGQWR010000000
VERSION     DAGQWR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.170"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(18..1658)
                     /gene="bclA2"
                     /locus_tag="MIW95_003389"
     CDS             complement(18..1658)
                     /gene="bclA2"
                     /locus_tag="MIW95_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBY3152686.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGATGATGA
                     NGITGPTGNKGATGANGITGPTGATGATGANGITGPTGNTGATGANGATGLTGATGAT
                     GANGITGPTGATGATGANGVTGATGPTGNTGATGPTGSIGATGANGVTGATGPIGATG
                     PTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGLVGPTGATGATGV
                     AGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGATGANGVAGPIGPT
                     GPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGPIGPTGPTGANGTTGATG
                     ATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLGNNTLVTFNSSFINGTNITFP
                     TSSTINLAVGGIYNVSFGIRAILSLAGFMSITTNFNGVAQNNFIAKAVNTLTSSDVSV
                     SLSFLVDARAAAVTLSFTFGSGTTGTSPAGYVSVYRIQ"
     gene            complement(2036..2575)
                     /gene="hpt"
                     /locus_tag="MIW95_003390"
     CDS             complement(2036..2575)
                     /gene="hpt"
                     /locus_tag="MIW95_003390"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBY3152687.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2903..4210)
                     /locus_tag="MIW95_003391"
     CDS             complement(2903..4210)
                     /locus_tag="MIW95_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBY3152688.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDEKLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4635..6410)
                     /locus_tag="MIW95_003392"
     CDS             complement(4635..6410)
                     /locus_tag="MIW95_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3152689.1"
                     /translation="MMVMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDVCRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6407..6544)
                     /locus_tag="MIW95_003393"
     CDS             complement(6407..6544)
                     /locus_tag="MIW95_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152690.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6622..7191)
                     /locus_tag="MIW95_003394"
     CDS             complement(6622..7191)
                     /locus_tag="MIW95_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBY3152691.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7375..8010)
                     /locus_tag="MIW95_003395"
     CDS             complement(7375..8010)
                     /locus_tag="MIW95_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBY3152692.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2327 a         1643 c          951 g         3253 t
ORIGIN      
        1 tcttttcatc atataaacta ttgtattcta taaactgata catacccagc tggagaagta
       61 cctgtcgtgc ctgaaccaaa tgtaaagctt aaagtaacag ctgctgctct agcatcaact
      121 aaaaagctta aacttacact tacatctgat gaagtaagcg tatttactgc ttttgcaata
      181 aagttatttt gggctactcc attaaagtta gtagtaattg acataaatcc tgcaagtgaa
      241 agtatggcac gtataccgaa agatacattg tatatccctc caactgcaag atttatagta
      301 ctacttgttg gaaaagttat attagttcca tttataaatg atgaattaaa tgtcactaat
      361 gtattattcc ctaaacttga agaagatact gtaaattgtg cattgtttgc tgctaacact
      421 cctgttgctc cggtcgctcc tgttggacct gttgctccat ttgctcccgt tgctccggtc
      481 gcccctgttg ctccggtcgt tccatttgct cctgttggtc ctgttggacc tattggacct
      541 gctactccat ttgctcccgt tgctcctact gctcctgttg ggcctgttgc tccatttgcc
      601 cctgttgctc cggtcgctcc tgttgctcct gctactccat ttgctccggt tggacctgtt
      661 ggacctattg gacctgctac tccattcgct ccagttgctc ctactgctcc cgttgggcct
      721 gttgctccat ttgcccctgt tgctccggtt gctccggttg gacctactgc tccatctgct
      781 cccgttgggc ctgtcgctcc tactgctccg gttggaccta ttgccccagc tactcctgtt
      841 gctccggtcg ctcctgttgg acctaccaaa ccatttgctc cggttgctcc ggttgggcct
      901 gttggaccta ccaaaccatt tgctccggtt gctccggttg ggcctgttgg gcctgttgga
      961 cctaccaaac catctggacc tgttgctcct actgctccgg ttggacctgt tgctcctatt
     1021 ggacctgtgg cacctgttac tccatttgct cccgttgctc ctatacttcc ggttggacct
     1081 gttgctcctg tatttcctgt tgggcctgta gcacctgtta ctccatttgc tcccgttgct
     1141 cctgttgctc ctgttggacc tgttatccca ttcgctcccg ttgccccagt tgctccggtt
     1201 agtccggttg caccatttgc tcccgttgct cctgtatttc ctgttggacc tgttattcca
     1261 tttgctcccg ttgctcctgt tgctcctgtt ggacctgtta tcccattcgc tcccgttgct
     1321 cccttgtttc cggttggacc tgttattcca tttgctcccg ttgctcctgt tgctcctgtt
     1381 ggacctgtta ttccatttgc tccagtcgct cctgtatttc ctgttggtcc tgtagaacct
     1441 gttgttccat ttgctccagt cgctcccata tttcctgttg gtcctgttat tccattcgcc
     1501 ccggttgctc ccgtatttcc tgttggtcct gttattccat ttgctccggt cgctccggtt
     1561 gcgcctctag gccccgtcgg tcctgttgga ccagtagcac ctgttggccc tgttggacct
     1621 acatcttgat ataaacttgg acctgaaata tcactcatat aattaatcct ccttttttaa
     1681 agttagagta ttactctata aatattgagt attactctat ataattatat gaatcgacaa
     1741 tttcttatgt gataatgtat ctatttgctt atatcaacat atatgctttg attcttaaat
     1801 ttcattactt ttttgtaaaa tcttttacaa ttattagtac tcttttacaa ttattaatac
     1861 aattaaaaaa ctcatttccc atcaattatt taaaattttt ttattatgtt ataaaaaata
     1921 gataattaaa ttgcataaaa ttaatttaat actactttaa ttacttttta aataaaaaaa
     1981 cctcaacacg attaataaaa atcatattaa ggcatccaca aaattatata attgcttatc
     2041 tatcttcatc agtaacattg aatatataag gaacatttct ataataatca ccaaaatcaa
     2101 acccatatcc aaccacaaac ttatcttcta tttcaaatcc acaataatct ggcactaaat
     2161 caacttttct tctgcttggt ttatccaaaa gaacacagca ttttaaacta gcaggatttt
     2221 tagcttttag atgttttaac acaaaatcca tagtaagtgc agaatcagta atatcgtcta
     2281 caaccaaaac atcatatcct gctatatcag tagtcacatc tgaaaccact ttaacctttc
     2341 cagtactttc ttcattgttt ccataactag atgtagtcat aaaattaact ctaagtggta
     2401 aatttatatt tctaactaag tctgcacaaa atataaagct accttttaat agagaaacta
     2461 ccataagatt tttatcttta taatcttttt ctataattgc tcctaattcc cttaatttat
     2521 ctttaatttt ttcttctgaa tacaatactt cccatttttt tgtttctata tccataaaaa
     2581 gacctccaat tgtaataatc cttcttaatt ttataagaga ttgctcactt tttcaatact
     2641 cttattcaat attagtcaat attattgagt ttttatgttc attcaacaat tattaacaat
     2701 gtatcttata ttttaagtag ttcatatatc cacactaaat tacatactat atattttaga
     2761 caaaaaaaga aaaaacttgc actaacaagt tttttctatt atacataaat ttatacataa
     2821 tcatatttta ttagagggta ctaattaaat ttatttatgc tatttttgtt atacctcata
     2881 atacaaaaca tattataaag tactagtcaa ctttattcat atcattcatt acatttaaga
     2941 ttatctcatc tgttatagac atacctttgt cacttactct acctaagttt tgtatacttt
     3001 gctcaacact tgaaccaact ataccattta gtggtggtac aaaacaatct tgcttagcta
     3061 ttatagcact ttgtatagct gcagatgctg cagaagataa ttttaatgca caaccagctt
     3121 tcgcaccgtc acatatcatt ccacttaagt cagctatcat attttctata gctccttcta
     3181 tttgcttttc agtaccattc ataagccaag agataccagc agttgctcca gtagaagcag
     3241 caacaccaca tccacacaca gcagataatc ttcctgtata gttcttaata taaccagtta
     3301 ctagatgaga tatagctaaa gcctttgcta gtttttcatc agattgtggg aattttttat
     3361 tataagcaac gattggaaga attgctgtta atccatgatt tccacttcca tttgaactca
     3421 taacaggcat ttttacgcct gccattcttg catctgaagc tccagcagtt aacatcattg
     3481 cataatttat aacatcatta cctaaaagac cttcttctat agacttttta attccatatc
     3541 cgacaccaat accagttttt tgttttaatc catattcagc catctcttca ttcattttta
     3601 ctccatctag taaaaattct atatctttaa aatcaatttc ttctacattt tttattaatt
     3661 cttgtatagt tactgtatcc attaaacttt cagctttttc tgccgcatca tttgaagctg
     3721 atacttttgg ttcattgtct aataatactt ctccatcttt ttctaagaac gtaaaattat
     3781 catgtttagt tcttattctc actttagcaa tatgattttt tccttttaac acaacttcta
     3841 taaatacttt ctctcttgta tcagcaggtg ttattgatag tggagtatta tccatataat
     3901 cctcagctat tttaacttct tcttttgtta gagtttctaa cacacttaat ccattttcag
     3961 aatggccacc tactatacct agagctgctg caatttttaa tcctaatctt tcagtacctg
     4021 gtatacctac gcacatgcca tttttgtaga tactaggact aactaacata cgattttcaa
     4081 ctatttcttc tcctaataat tcctttgcct ttgcacatgc tagagctaag gctactggtt
     4141 cagtacaccc tactgctggt ttaacttctg cctttaacat ttccactaat tcttttctta
     4201 tatctctcat tatatataaa cctcccaatt aagtttagat ttagtactca aataattaat
     4261 taaataattt cttatctata tttatagcaa aatacatgcc atatttttta tatcttttta
     4321 tatttttagt cttatattct ataaccattg gtatgactgc ttctatttat gtattttttt
     4381 tatttacata gagtatttat attctttaaa atggagaaac atatgacaat ttcgcaaaaa
     4441 gacaaaaaaa agtctcagaa tgagattcta tatcattttg agacggctca aatgtgttat
     4501 tttttaactt ataaaaacca ttttttaatt cataaaaaaa gattatgcaa aacacttata
     4561 gtataagttt aaagctttct ttaaagttat gaatttagaa aaatttaaat tttatatagc
     4621 tttttattat attactatga aattatattg tatttttcaa tttttctata taatgtagct
     4681 cttgatattc caagtgcttt agctgttaat tctttatctt ttttataatc tttgtactta
     4741 tttaaagctt ttattatctc aatcttctct agttcatcaa gtggaattat cctatcgctt
     4801 ttaacttcta actcttctac agaggtattc ttaatttttt gaggaattac attcattgtg
     4861 attattgaag aagaagacat attcacacta tattcaataa tattctgcaa ctctcttata
     4921 ttccctggcc acttatattc catcatagca tcaacaaccc tgctatctat gtcaataaca
     4981 tttttatcta atttttttga atactcttta accatatgct tgattataag tgggatatct
     5041 tcttttctct ctcttaaact tggtaatttt ataggtataa catttaatct ataatataaa
     5101 tcttctctaa aagtgccatt ttgaaccatt ttttctaaat ctttatttgt agcagcaata
     5161 attcttacat ctattgagac atttgattta cttccaattt tattaagttc cttttcttgt
     5221 aatactctca ataactttga ctgtaaatgc aaactcatat ctcctatttc atctaaaaat
     5281 atacttccct tatgagctac ttcaaacatt ccaagctttc cacctttttt agctccagta
     5341 aaagctccct cttcatatcc aaataattca ctctctaata aattgtctgg tatagcagca
     5401 cagtttacag ctataaaagg attatctact ctattgctat ggttgtgtat ggctcttgca
     5461 aatagctcct tacccgttcc actttcccca gtaataagta ctgttgatgt tgatttagaa
     5521 gctattaggg cttccttttt agaatttctt atagctacac tttctccaat tatgttttcc
     5581 aattttattc tataatcttt attcatctta ttgtagtttt ttatagcatc tcttttatct
     5641 ataaaatcaa taacatatcc ctttaactca ttttttaata ctattttatt gatgttgtat
     5701 attcccttcg aatcatatcc gtatctttta taaaaaaaac tacacgattt gtctttctta
     5761 aagttattta tagaaggttt ctttataaaa tctaattcga caaatatgtt tttgccagtt
     5821 atactatcct ttatattgaa aagctcttta aattttgaat tatatttatc aatttttcca
     5881 tccacatcaa tagacacaac agctttgtcc atattgttta atagtgtctc taattttttc
     5941 ttttctaatt caagctcata agttttaatt tgtgctttta atttattaga tattaagtct
     6001 gccattttgc ctaaaaagtt tataagacca cttttattat tttctataat ttgtgcttgt
     6061 tcttttgtaa aggctataag gcctataacg ccatgagaat cgccatcaca tataatagga
     6121 caacacacct ctgcaaactc tacacaagcc ccacttttat agcattccct acatacatca
     6181 ctactctttg ggtcatcaat tagtattgtg atctgttctg taagtgattt tttaaaagcc
     6241 gtatatttat ctactttctc acctattttg tctatatata ctcctgtacc tgcaatccta
     6301 aataactcct tatttactat agttacatct atattgagta ctgatactat agcttcagct
     6361 atattttgaa tatcagattt tatatattct aaacctatca taaccatcat tccttatttt
     6421 tctttaacat aagtatttgt cttatttctt taagaatcaa gtatatctta tttaaagtaa
     6481 attggattga catcaggatt acaaagagca taacaattat tattaactca taatttattt
     6541 ccatctaaac atctccttat aacaaaagac taggcttaac ctagtctttt atattatatc
     6601 catatttata ttttaactca tttatcttct ttttcccaca taaatatatt ttaatttatt
     6661 tgactcttta atcataccat caaagtttaa tcttgaaaat atattaaata cttcatgtgg
     6721 atagtgaatc tttgcaacag aaccacaaaa tttatgttta agataattta acttaacttt
     6781 taaattcttt aaatttaaat cctctaatat aacctctcca ttgcccttta acactctttg
     6841 tatttcagat gaagcttttt cacaatctgt aataacatta aattggtcaa ctattattat
     6901 cttgttgaac tgattatcat caaatggtag aaactctaac ttacctttaa ctacattgca
     6961 attttcatta tacactagag aataacagac atcttcataa ttactattct ccagtatagt
     7021 tacagaagtt ccaagacctc tcaatctctt gcctaggttt cctatatgac caacaagcaa
     7081 tattttatca cctacattat actcaattgt atccaaaaaa cattctaatt catcagatgt
     7141 atatttagct ttaactggct gtttatttat ctgacataat atgcttttca caatgcttca
     7201 cctcttcatt tcctcaatta taaattatgt acagattaca ttataataaa tggcgagcaa
     7261 ctattcaaga gatatttcat tagtattttt ttaaacaatc tttatacatt ttaatatgat
     7321 attataccaa atttattagg attttattca taaaaatcag tctatttttt atgtttattc
     7381 attgttattt tcatccaatt tttttatttt cgatatggat acttcatatg ccattttttt
     7441 gaccacatta ttttcatcta tttttttctc atattctcta ctttgaaccc taccccaaag
     7501 ctgtattcta tcaccaacct ttagattctt tgcaaattta gcatttctac cccaaactat
     7561 tgatggtata taatcagatt tattataagg tctgtttata gcaactaata aatcggttat
     7621 ttcccttcct aaaggtgtct tcctatacac aggttcctta catacatatc catctaaaaa
     7681 tatactatta gggtctttat tttcctcgtc tatgcttttt atttctctga caaacactgt
     7741 aagcactaat ctatttttat tatttaatgt tttgttatat gatcttaatt ggcctacgac
     7801 attgactaaa ttttgtttat caaggtcaat atcttggaga agtctttctg atacagtcat
     7861 tggaagtata tcaaaagaat cgcttagtct gtttattttt attttcatat tataaaattt
     7921 ttctccaaat atctcatggc taaattctag cttattgtct aactcccctc ttaagtttac
     7981 tacgttattc tcatctttaa cagctatcat attttatccc tccacaattg ttaattctta
     8041 tatttctggt atcagcttat ataataaata tatttataag ggatgtattt tattacaaat
     8101 ctttgattgc tgtttaaaag tttttattta gttataaatt tcatttaata tttcactttt
     8161 ataattataa aatt
//
LOCUS       DAGQWR010000169         8171 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.171, whole genome shotgun sequence.
ACCESSION   DAGQWR010000169 DAGQWR010000000
VERSION     DAGQWR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.171"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..597
                     /locus_tag="MIW95_003396"
     CDS             <1..597
                     /locus_tag="MIW95_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY3152693.1"
                     /translation="GLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQS
                     LSLIMSCLTSPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALH
                     YGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            728..1750
                     /locus_tag="MIW95_003397"
     CDS             728..1750
                     /locus_tag="MIW95_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY3152694.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRIGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(1914..2471)
                     /locus_tag="MIW95_003398"
     CDS             complement(1914..2471)
                     /locus_tag="MIW95_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBY3152695.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2477..3553)
                     /locus_tag="MIW95_003399"
     CDS             complement(2477..3553)
                     /locus_tag="MIW95_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBY3152696.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3554..4237)
                     /gene="modB"
                     /locus_tag="MIW95_003400"
     CDS             complement(3554..4237)
                     /gene="modB"
                     /locus_tag="MIW95_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBY3152697.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4386..5198)
                     /gene="modA"
                     /locus_tag="MIW95_003401"
     CDS             complement(4386..5198)
                     /gene="modA"
                     /locus_tag="MIW95_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY3152698.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKTDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEVESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5391..6320
                     /locus_tag="MIW95_003402"
     CDS             5391..6320
                     /locus_tag="MIW95_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3152699.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6481..6930)
                     /locus_tag="MIW95_003403"
     CDS             complement(6481..6930)
                     /locus_tag="MIW95_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152700.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARVLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7133..8113)
                     /locus_tag="MIW95_003404"
     CDS             complement(7133..8113)
                     /locus_tag="MIW95_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY3152701.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVICGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2829 a         1341 c          984 g         3017 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaagta ttattaaata aacataactt gtctaattta
       61 gctgaacaca gaagaactca aatagtaagt agagtttttc aagacccaag tcttggaact
      121 tgcccttcta tgactgttag agaaaacttg tctttagctc ttaacaaagg aaaactatta
      181 aatctaagaa aatgtcttcg ttacaaaaga gatttcttag aaaatttatt ggaaggtgta
      241 tctcttgatt taaaaaaata ccttgatgtt caagtaaaat ttttatcagg aggtcaaaga
      301 caatcattat cactaattat gtcgtgctta acaagtccat cagttttatt acttgatgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa ttgaattgac taataagatt
      421 gtaagggaaa aaaatataac tacacttatg gttactcaca acttaaaaca cgctcttcat
      481 tatggtgata gattgataat gcttcataag ggtgaagttg tattagatgt aaaaggtaaa
      541 gaaaaagaac aattaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtttaaaat
      601 aatatatcta ttgtattaaa ctcggcttta aaataaagtt aaaattacaa taattatggt
      661 tactttgtaa taattcatat gaatatatga acatatataa taataaattt ttgaggggga
      721 tataaaaatg attaataaaa aaagattggc aagtttgatt ttagctggtg ctcttagtat
      781 atctatgctt acagggtgtt cacaaggagg agactctggt aattcaaaac aagaaagtaa
      841 ttctaaaggt aaagaagtta aaaaaattgg tataactcaa ctggttgaac atccagcatt
      901 agatgccact aggataggtt ttgtaaaagc attagaaaag aacggattta aagatggaga
      961 aaatattgat atagacttcc aaaatgctca aaatgacatg cctactacac aaagtattgc
     1021 gagtaaattt gcatctgata agaaggactt aatatttgct atatctactc catcagctca
     1081 ggcagcattt aatgctacta aagatatacc tatacttatt actgctgtga gtgaccctgt
     1141 tgctgctgga ttagttaaaa ctcttgaaaa gccaggcaca aatgtatctg gaacatcaga
     1201 ttttgtttct gtagacaaag gtttagaact tcttaaaata tttgctccta aggcaaaaac
     1261 tataggagtt atgtacaata ctagtgaagt aaattccaaa gttcaagttg atgctttaaa
     1321 agaatatgca tctaaaaatg gatttaaagt tgttgaaaaa ggtataacta cttcaaatga
     1381 agttaaccaa ggtatttcta gtttagttgg caaaatagac gtattatatg ttcctacaga
     1441 taacttagtg gcttcttcta tgcctatagt ctctaaaata gctactgaga ataaaatacc
     1501 tgttatagca gctgaatctg ggccagttga aaaaggtgct cttgcttgtc aaggtatcaa
     1561 ctacgaaaaa ctaggttaca aaacaggtga aatggcagtt aaaattttaa atggcgaatc
     1621 agtatctgat atgcctgtgg ctacatcaga tgatacagat atcattgtta atgaagatat
     1681 attaaaagct ttaggtatgg aaaaaccttc taatgaaaat atttcttatg taaagactaa
     1741 acaagaatag aaagatattt tctatatacg attaatatgc ctgtttctat aaattaattt
     1801 atagaaacag gcatatattt ttattttaat gtattatcta tttaaagatt atatgtaaat
     1861 gctcttaata tgattgtata aattttactt gtataaactt tatacaatca tatttattct
     1921 tctattttct ttattactct acatggattt ccaacagcta cagtatttgg tggtatatct
     1981 tttgtgacta cacttcctgc acctattact acattatcac ctatagtaat tcctggtgtt
     2041 atgattactc ccccacctat ccatacatta tcacctattt ttacaggaga accatactct
     2101 atgcctgaat ttctaagttg tgcatcaatt ggatgatagg ctgtatagat ttgaacgttt
     2161 ggagctaaca ttacattgtc acctatttca attttacata catctaaaaa aatacaatca
     2221 taatttgcaa agaaattttc gcctacatgt atgttatatc cataatcaca cctaatgttt
     2281 tgttctacat ttatttgctt gccaactgaa ccaaatagtt gtcttaaaat atcttctctt
     2341 ttttcatatt catcctctaa agtattatta aacaatctag ttaatttttt acaatactct
     2401 ctttctttta ctaacaattc atcatttgca tagtatcctt ttccagacaa cattttttct
     2461 ttttcagtca tatagtttac tcctttttgt tataattgta tctaaagcaa aataaatatt
     2521 cctgtggaaa atctaaatat attctgtcac ctttcttaaa tctcatttta cttttttcca
     2581 attctatttg aataggtaca cattcgttag ataaatcagt ttttctaaca tatatagtat
     2641 atgtaaaagg attttctaca atgttaatca attcgaaaca taacttttca tttaaatctt
     2701 cattagaatc caagacctta atatgatgct ccctgattcc cacatattct atattatcac
     2761 ctatttctct atttaatgtc aactcgcatc cccagtctgt agcataaata gtatttttat
     2821 ccagtctatt taatttggat atatttttac accctgttat cttagcttct ataaggtact
     2881 ttggagattc aaagagctct tttttaggtc tctttggaag accttctcct ttgttgtaca
     2941 ctataatatc atcacaaact cggtaagctt cttcaatatc atgagtaaca aatagtatat
     3001 ctcccttgta atcttttaac atattcataa gttctttttc catattactt cttaaatgat
     3061 ggtccagtgc tgagaaaggt tcatctaaaa gtaatatatc tggagatgtt gccaatgctc
     3121 tagctagtgc taccctttgt tgttgaccac cagataattg ccaaggatat ctcccttcaa
     3181 acccatttaa tttaagtata tctaagtacc ttgctactat ttcctttttt tcactcttac
     3241 ttagttttaa aagcccaagt tctatatttt gacttacagt catatgtgga aacaaagcat
     3301 agttttgaaa taaaaatcct acctttctat cttgtgttga taaatttatc ttcttttcag
     3361 aatcaaatag aactctatca tttaatacaa ttttacctct tgttggtttc tcaagccctg
     3421 ctatacattt taatgtcata ctttttccag aaccagattc ccccaaaaac cccagagtac
     3481 ctttttcttg tttaatttct acctttagtt taaaagagga taaatctttt tcaatatcca
     3541 cgtataagct caactatcca cacctctttc ctataatttt ttgctgaaat tccgaccaat
     3601 aatttagtaa taaaatcatt gctatagata ttgctacaat aatcataacc cacatcatag
     3661 ctttattcat atctccgcct tcaacagcaa aaaatatagc tattggcata gtttgagttt
     3721 ttccaggtat atttccagct atcataagtg tagcaccaaa ttcgccaaga gctctggcaa
     3781 acgacagcac caatccacca attattccag gccatgctaa tggaactgca atcttaaaaa
     3841 atactttcca ttcattaaga cctaatgttc tagcagctga cagcatatta ttatcaatct
     3901 gttcaaaagc tgaccttgaa gttctataca tcataggaaa agacaccact actgctgaaa
     3961 ttacagttgc tgtccaacta aatattaaag tcctatcgaa atttaataaa aactttccta
     4021 taaatccatt cttcccacat agtaataata aaaagaaacc tacaactgtt ggtggcaaaa
     4081 ttagtggaag tgtaaatagt ccatctataa gacccttcca cttacctcta tagttagcca
     4141 taatatatga cacaaatatt cctattacaa aagttattat tgttgacaaa atagatgtct
     4201 taagtgatat ccataagggt gaccaatctg ttcccatctt tccctcctta attttaaatg
     4261 gaaatagtga aactaataac tctattaatt tcactatttc cattttatta taatacacaa
     4321 ttattttcaa ttttcattta tttatatata atttatactt aattacacat aatttattta
     4381 taagtttatt ctactttttt atatccaaat tcttcaaaaa tcttttgtcc tgactcactt
     4441 aataaaaatt cttcaaattt cttagcagca tctacgtttt tactagcttt aattactgaa
     4501 actggatatg taataggtga atgagttttt tcgtctgttt tttcaactac ttttatctta
     4561 tcattattga cagtatcact gaagtaaacg aatccaacat cagcatttcc tgattgcacc
     4621 catgctaaaa cttcttttac atcttttgca aatactagct tatcttttaa cttatctttt
     4681 aaatttaaat tagttaacac ctcatctgca tacttaccag ctggaacact ttcaacttca
     4741 ccaacagcaa ttttcttcac tttatctgta gtcaaatctt tcatacctga tacactactg
     4801 tcagcagaag aaataagaac taaatcattt tttactaaat ctttcatagt atcagaaact
     4861 aataacttct cttcatccaa agctttcatt tgtttttgac ctgctgaaat aaataaatca
     4921 caaggtgctc cttgttctat ttgttgttgt aatgagcctg atgctccata gttaactgta
     4981 agttttacat ttgaatcaac ttttttatat tcttcttcaa tctttgccat tgcttctttt
     5041 aaacttgcag cagccgatat atttaactct actgaatcac tacttttagt tgttttttct
     5101 tgttttccat catctgtttt gtcttcatta ctattgcacc caactgtccc caaagttaaa
     5161 catgcaacta atcctaatat tcccaatatt tttttcataa caatctccct ttttgtaaat
     5221 ttgatacaca ttttaatatg ttttattatg ttttatcatg ttttgttata tatagtttaa
     5281 ttatatatac atgataataa attttcaagt aatttattta cactactttt aaatattttg
     5341 ctacattttc ctttttttaa tgttaaaata accttatatg gaggtggatt atggactcat
     5401 tatcatcttt gacacctgtc gaggtagctg aattattaaa gataacaaaa aatactgtat
     5461 atgaattaat caaaagaggt gaacttcctt cttataaagt agggaaaaaa ataagagttg
     5521 atactaaaga tgttgaagaa tatatcaaca gccaaaaaac tggtaaagta aaggatacac
     5581 atttcaacaa taaaaatata gatataaata ataatttcaa aattattatt tctggtcaag
     5641 atgtaatatt agacatttta gctaggtctg ttgaaaaaaa attggatgga ataagtacat
     5701 ttcgctctta tataggtagt tataatgctt tgtatgagtt atataatggc agagtttcta
     5761 tagcatcatc ccatctatgg gatttcgaaa cggatgaata taatagcact tttgttaaaa
     5821 agctattgcc aggaatacca tgtgtactta taaatctagc ttatagaatg caaggtttct
     5881 atgttgctaa aggaaatcct aaagagataa caacctggga ggatttaaca aaaccaagta
     5941 taaccattgt aaatagggaa aaaggttctg gaactagggt tttactagat ggtaaactta
     6001 gacttctaaa ctttaatggt aaacatataa aaggttatga taatgaagaa ttatcacatc
     6061 ttggagttgc aagtactgtt tctcgtggaa tagctgatgt tggtttggga aatgaaaaag
     6121 ctgctttaca agtaaataat atcgacttta ttcctcttca caaagagcgc tatgatttag
     6181 ttattaaaaa agaagactta caaaatccag tttaccaaac tataattaac ataataaact
     6241 ctcctgagtt taaagctgaa cttcaaggtc ttggcggata tgatttaact gatactggaa
     6301 aaactatagc aaaagtttaa gattaaaatc gcaatctatt ttataagatt tataattttt
     6361 cacaaaattt ataacttaaa cacgaaataa attgcgattt tagtgtctca aaaaatttat
     6421 gtctttcatt ttatcatcca atacaaaata taatataatt aattgtgcaa tcatattata
     6481 ttaatatata tttacataat agtatttttc tttaatgtag attaacatat gatatgtttt
     6541 aaacatcaac acccttgctt tcttatatat atgtggcaac acatatgagt ttaataaatt
     6601 atatacaatg ttgctttctt cttgtatttt ttcaaccaac aagttattta taaaattacc
     6661 tttctcattt tcaataatct cactccaatt tatcttaact tttaatatat atcttcccac
     6721 taggtataca cactctacaa taaataataa aaatacaata cagtcagtac ctgttgtctt
     6781 atataaatta cccgatacta atggctttgc gaagaaatta taaaaattca aagtatcacc
     6841 cgtataatca aaaagtatta atagcactcc tagaactctc aatactttaa tattcaacat
     6901 gtttttatct ttattatttt tgtttttcat atatactacc tctcttttct ttagatttga
     6961 ttgtaatcta atactaactc ttttatttga ggtataaatg aatttaattg gtagttattt
     7021 tcatttttac tagaaattaa ttcacattac taaacaaaac ttatatttaa acataaaaat
     7081 agcctatcta ttttttaata aatacaaaaa taaataagct actaatattc aattaacttt
     7141 tatcacttaa aacattacta tctataaatt taaatatttt attgaagtat actgtactat
     7201 catgttcaaa tccatcaaca tgaccaatac cttctataag ccataggtct tttttatcat
     7261 gtttaactaa atcatacatt ttcttgctat tttgataatt acaaacagta tcttttgttc
     7321 catgtataaa cagtatgggt acattagatt tctcaactga ctcatatggt tttacatcac
     7381 tgtaccaaaa tccagattta aacttattat ataaatctcc acaaatcacc acataattga
     7441 ttaatatatc tggtattcgt ttttctagta ctcccattct aattgcatct ttcatttcag
     7501 aataaggaga atccagtata taaaaatcga ccttatcatc ttttgaattt atctctgaat
     7561 gcatagcagc tgtccctgct cccatggaaa atccatgcac tcctaatctt ccttcaggaa
     7621 atttattttt tacaaattta actagactat ctaaatcata cctttcataa agaccaaatg
     7681 tgtaattatc tccaccacta ttaccagtat ttctttgatt ataaactaat acattataac
     7741 ctttatctaa atatctatat gctaccttta acatttcata gtaactactt ccaattccat
     7801 gaacaagtat catagtatct cttgtcttta tatttgaagt tataaaaaga ctttcaagct
     7861 tgtaattatt agaactttgt atcattaatt cagatgtctt gtagtttttt aatttctcca
     7921 gtggtttatc ttttctctca gaaaatattt ttactatatc ttcgtttttt attaactgct
     7981 ctaaaccaac cgaactatta tacactaatt tgccaaatat ataagataca attaatagga
     8041 ttaatagaaa cacaattgat ataattataa atttatttct taaactaata caatttttaa
     8101 aactattttt catatttatt taccatacaa ttttcctata ttatattttc taagtaatag
     8161 cataattgat t
//
LOCUS       DAGQWR010000170         8113 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.172, whole genome shotgun sequence.
ACCESSION   DAGQWR010000170 DAGQWR010000000
VERSION     DAGQWR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.172"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            179..640
                     /locus_tag="MIW95_003405"
     CDS             179..640
                     /locus_tag="MIW95_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY3152702.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            708..2954
                     /locus_tag="MIW95_003406"
     CDS             708..2954
                     /locus_tag="MIW95_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152703.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            2947..4779
                     /locus_tag="MIW95_003407"
     CDS             2947..4779
                     /locus_tag="MIW95_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152704.1"
                     /translation="MSRMGRGPMGKSMEAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            5498..6775
                     /gene="hflX"
                     /locus_tag="MIW95_003408"
     CDS             5498..6775
                     /gene="hflX"
                     /locus_tag="MIW95_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBY3152705.1"
                     /translation="MDMTKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELYDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDCVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            7008..7505
                     /locus_tag="MIW95_003409"
     CDS             7008..7505
                     /locus_tag="MIW95_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY3152706.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            7555..8079
                     /locus_tag="MIW95_003410"
     CDS             7555..8079
                     /locus_tag="MIW95_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBY3152707.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKDLQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKVPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILNYIRKL"
BASE COUNT         3223 a          832 c         1468 g         2590 t
ORIGIN      
        1 aagtagctga ctaattaaaa atcttactat tatttattaa aattataact atatgatata
       61 aaaatattta ataaatgaaa tttatatgaa ttttttataa atatattgaa aaaatgcatg
      121 cagtatagta aaataatgct tagccgacta agtaatataa aaaataagga gggcaagtat
      181 gaatagcaat tctgaagaaa aattaaatga tgttttcttg caattaatgc gaatatattt
      241 tataagaact catttttatt ttgataaatt aggcttatat catggtcaac cccaaatact
      301 tttttcttta aagatgaaag atgggcaaag tcaaaaagaa ttagcagata aaaggaaagt
      361 aaaagcatct acaactacag ttatgataaa gaggatggaa aaagctggac ttgtggaaag
      421 aaaacaagat gaaaaggacc aaagagtatc taggattttt ttgacagaca aaggtttaga
      481 aatatgtaat aaagttgaag aatttaacaa ggaattggaa agagagtttt ttttaggatt
      541 tagtgaagaa gaaaaaatag tacttagaag acttctaatg caagtacgag ataatttaaa
      601 agcaggatgt cataagcatg aagatgaaaa atatttttaa agtttaaaat aatgtttttc
      661 aatttaatgt tattagacta attaatatta cagaagggaa gaaatgtatg tttaaattaa
      721 ttaaatattt aaagaaatcg gcattatcaa tagtaataat agtctgtcta ttggtaattc
      781 aagcggtatg tgacctttct ttaccagaat acacatccaa tatagtaaat gtaggtatac
      841 aacaaggtgg agtagaaaac tctgttccat ctgtgattag agagagtgaa ttaaataaaa
      901 ttactttatt tatggataaa agtagtaagg ataaagtact agataattat actttgctga
      961 ataaaaagga ttatgttaag tataaagata agtacccagg tctaaaagat gaatctcttt
     1021 atgagttaaa tactaaagac aaagacacta tagatgattt aaatgttatt tttggtaaag
     1081 ctattttaat agtatcaggg cttgagggcg atagtaaaga ggtaaaagct atgaaagctc
     1141 agttaatgag taaacttcca ccacaagcta ctcaaagtgg agatgttgat atatttaaat
     1201 tgttatcagc aatgccaaag gaacagttag atacattgac taaagagatg agcaaaggct
     1261 ttgaaagtat gccagagagt atgataacac aaagctctgt ttcatatgta aggtcagaat
     1321 atgagaaaat aggtattgat acagaaaaaa ctcaaaataa ttatatatta tttactggtg
     1381 ctaaaatgct tggtatagca atgattagta tggtagctac aataacagta ggatttttag
     1441 cagcaagagt agcagcaagt gtaggtagaa atttaagaag tggagtattt agaaaagtta
     1501 tgtcattttc aaatactgaa atgaatgaat tttctacagc ttcattaatt actcgtagta
     1561 caaatgatat tcaacagata caaatgttga tggttatgtt gcttagaata gtattctatg
     1621 cacctataat ggctataggt ggagtgataa aggttttaaa cacaaatact tcaatggcct
     1681 ggattatagc agtagcagta gttgctatct tatcattaat tgtagtgtta ttctcagtgg
     1741 ttatgcctaa gtttaaactg gttcaaaagc ttgttgataa gttaaactta gtaagccgtg
     1801 aaataattac tggtattcca gttatcagag cttttagtac tgaaaaacat gaagaagaaa
     1861 gatttgataa agcaaataaa aatttaacta aaacaaatct ttttgtaaat agagttatga
     1921 catgtatgat gcctgctatg atgcttataa tgaaccttat cacagttctt atagtttgga
     1981 gaggtgcata cagtgttgat tctggagcaa tgcaagttgg agatatgatg gcatttatac
     2041 aatatacaat gcaaataata atgtcattct taatgatatc aatggtatct attatgcttc
     2101 caagagcatc cgtttcagct acacgtatag atgaagtatt agtgtcagat ataagcataa
     2161 aagacccaga aaaatcagag gcacaagagt ttaaggaaga taaaaagggt cttgttgaat
     2221 ttaaaaatgt ttcgttcatg tatccagacg cagaagaacc tatattaaca aatatatctt
     2281 ttacagcaaa gccaggagaa acaactgcct ttataggaag tactggtagt ggtaagtcaa
     2341 ctcttataaa tttaattcct cgtttctttg atgttactga aggagaaata ttacttggtg
     2401 gagttaatat aaaaaatgtt tctcaacatg atttaaggga aaaaattgga tatgtacctc
     2461 aaaagggagt attgttttct ggtactatag aatccaactt aaaatatggt agaaaagacg
     2521 caacagatga agaaatgaga acagcagctg aaatagctca agctacagaa tttatagatt
     2581 ctaaaccaga agcttttaaa actgaaattt ctcaaggtgg aacaaatgta tctggaggtc
     2641 aaaaacaaag actttctatt gcaagagcaa tagcaaaaaa tccagaagtt tatatatttg
     2701 atgatagttt ttcagcactt gacttaaaaa ctgatgcagc tcttcgtaag gctttaaaat
     2761 caaaaactgc tgaaagtaca gttctaatag ttgcacaaag aattagtact atcttaaatg
     2821 cagaacagat aattgtttta aatgagggag aaatagttgg aataggaaca cataaagagc
     2881 ttctaaaaaa ttgcgaagtt tatgagcaaa tagctttatc acaactttca aaggaggagc
     2941 tagctaatga gtagaatggg aagaggtcct atgggaaaat ctatggaagc aggacaaaaa
     3001 gcaaatgact ttaaagggac tatgcgaaaa ctaattgcat atttatctaa atttaaaata
     3061 tcaataattt tagtaattgt atttgctatt ggtagtgcat cgttttctat agtaggtcca
     3121 aaaattttag gaaaggcaac tacaaagata tttgaagggt tagtgagtaa agtttctgga
     3181 ggaaacgtag gtatagattt taatgcaatt ggaaaaatct taaccttttt acttttcctt
     3241 tatttgataa gtgcactgtt ctcttttata caaggattta taatgagtgg aatttctcaa
     3301 aaagtatcct ataatttaag aaaagagatt tcagcaaaat tggatagatt accaatgaag
     3361 tattttgata ctaagacgca tggtgaaata ttatcgagaa ttacgaatga tatagatact
     3421 ttaaatcaaa gtttaaatca aagtatgaca cagcttataa catcagtaac aactatgata
     3481 ggtgttttga taatgatgtt atctataagt ggaattatga cattagtggc agttttaata
     3541 ttaccaatat caatgtttgt aatttcaaga ataataaaga aatcacaaaa atactttaga
     3601 tatcaacaag aatatttagg aaatgtaaat ggtcaagttg aggaaactta tagtggccaa
     3661 acaatagtga aagcatttaa tcgtgaagat gaagtaattg aagagtttga taaactaaat
     3721 gatagcctgt ataattcagc gtggaaatct caatttttat caggtataat gcaaccactt
     3781 atgatgttta ttggaaactt gggatatgta atggtatcaa tcttaggagg ttggcttgca
     3841 attaagaaaa caattgaggt tggtgacata cagtcattta tacaatatgt aagaaacttt
     3901 acacagccaa tgactcagat tgcacaggtt gccaatttat tgcaatcaac agcagcagct
     3961 tctgaaagag tatttgagtt tttagaagag gaagaagaag tccaaattgt tgaaaatgca
     4021 gtttcaatag atggtttaga aggtaaaatt gattttgaaa atgtcaactt tggatataac
     4081 ccaaacaaga ctataataaa tgatttctca gtaaatgtta aacctggtca aaaagttgct
     4141 attgttggac ctacaggagc aggtaaaact acaatagtta aactgttaat gagattttat
     4201 gatgttaata gcggttcaat actgattgat ggtcataata ttaaagattt taatcgtagt
     4261 gagcttagag aaatgtttgg tatggttctt caagatacat ggttatttag tggttcaatt
     4321 atggaaaata taagatatgg taagttgaat gctacagatg aagaagttat agaggctgct
     4381 aaatctgcac atgttcatag atttattaag acgttaccag atggatataa gatgaaatta
     4441 aatgaagaag caagtaatgt ttctcaaggt caaaaacaat tattgactat agctcgtgct
     4501 attttagctg atcctaaaat tttaatatta gatgaagcta caagttctgt cgatactaga
     4561 acagaagtat tgattcaaaa agctatggat aatcttatgg aaggtagaac aagttttgtt
     4621 atagcccaca gattatcaac tatacgtgat gctgatatga ttcttgtaat gaatgaagga
     4681 gatatagttg aacaaggaaa tcatgaggaa cttctaaaga aaggcggatt ctatgccaat
     4741 ctttacaatt ctcaatttga agaagatgaa gcaatgtaac aaaaaactat cttagcatag
     4801 acatgtaatt aataagataa aataaatgag ggtgccttaa aatgaacttt ttaattcata
     4861 aggcactctt ttgtgtatga aataaagtgt atttttatca ttttaacaat gaaaaagagg
     4921 attatctata ttttgagata gccacataat tttatgtctt tacaaagtgt ttattttatt
     4981 ataatatgta gtaaggaaaa tgttggaggt gattatcaaa atgtatattt aattttattc
     5041 tttaaatttt attataaata gaatttaatt ttaatagttg atgattatat tgaagtatat
     5101 gtttttatta cttttattaa tcatgcaaat aactacttaa atttaagaat agggttaaat
     5161 ataaaatgat aattacatat aagttaatta aactaatgta tttttaatat atatttagaa
     5221 ttttaatcct tcttatgata taagtagctt gttttgtatt acttagattt gtaaggagtt
     5281 tttttattga aaaaactgca taatatattt gggaggaggt gtaagaaagt gattttttat
     5341 ataagattga gaaaataatg cacgattatg gttattataa tagtaaataa gaagtgtcat
     5401 ttaaggataa gataaaaagc aaaattcttg atgaatttaa ttatagagcc ataatctcgt
     5461 gctatagatt aaataaaaaa tagcagataa ggagaatatg gatatgacga aaaaaggaat
     5521 aacagtagga ttaaacataa ataataaatc tgaggacttt aatgaactaa tgattgaact
     5581 tgaaaactta tgttcagcat gtgatattga tgttgtaggg agtataactc aaaatgcaaa
     5641 gcaggttaat agagcatttt acattgggac aggtaaagtt gaggaaatat taaacttaat
     5701 aaagaaagaa aacatagaga tagttatatt ttacaatgaa ctttctacat cacaactaaa
     5761 aaatcttgaa gaaaagctta attgtgaaat tatagataga acggcattga tacttgatat
     5821 atttgcacaa agggcaaaaa ctagagaagc taaacttcaa gtagaagtag caagtttaaa
     5881 atatatgtta ccaagactta taggttcaaa tgaaaattta ggacgtcaaa gtggtggagt
     5941 tggtactaaa aatagaggtt ctggtgagaa aaaattagaa cttgaccgta gaagaataga
     6001 agaaaagata acatctttaa atagggagtt gtatgattta aaattccaaa gagaaactca
     6061 aagaagtatg agaagaaaat caaatcttcc aaatgtagct ctagtcgggt atacaaatgc
     6121 tggtaaatct tcaataatga ataaactagt agatatattt aaaaactctg aagaaaagaa
     6181 agtgtttgag aaaaacatgt tatttgctac acttgaaact tctgtcagaa atatagttct
     6241 tgcaaataac aaagaatttt tattatctga tactgttggt tttgtaagta atctaccaca
     6301 tgacttagta aaagcattta ggtcaacatt agaagaagca tgtgaggcag acgtactgtt
     6361 acatgttatt gatatttcaa atccaagcta taaaagtcat attaaagtga cagaagatac
     6421 tttaaaacaa ataggtgcag atggtatacc tatgatacat gtgtataata aaattgattt
     6481 aatagatgta gaagtattgg atagaatttt agatagtatc gataaagaag gtatatttgt
     6541 ttcagttaaa aaagatataa atatagataa aatgataaaa tgtatttgtg atagcatatt
     6601 taaagattgt gtaagatgta agttcttaat tccatatgat aaaggtcatg tagtttctta
     6661 ttttaacgaa aatacgagca ttataaatac agaatacaga gaagatggag ctattttaga
     6721 tgtagaatgt agcaatatag aatataataa gtataaaaag tatgctttag agtaaatttt
     6781 gaaaaactta ttatagccta aaatatattt aatttataat ttattataaa ttgattgtaa
     6841 atatgatttt atgaaacggt atagccataa tgctatgccg ttttgccatt tataataaaa
     6901 ataaatttat tgacacaata gtaactaatt actataatat aaagtaacta gttactattt
     6961 aaaattatca aaaaattaaa atctaagata aagagaagtg tataataatg gatgcagaaa
     7021 gtttaatgaa aaaatatatg gtagatgatg atagtagaaa acaagctata tttgcaagta
     7081 tatttgtaat gcaaaataaa cttcaaacta catgtgataa attagaccca gaattaacaa
     7141 tgaaacaatg gttactttta gctattgcgg actctgtaga tgaaactctt acattaacca
     7201 aacttggaga attaatgggg tgctcaagac aaaatgtaaa aaaactagct attgctttag
     7261 aaaagaaagg ttttatacaa attgagcaaa atgaaaaaga ttcaagagct atctgtcttg
     7321 tgacaaaaga tagagtgcaa gagtattcta aaaaagtaag aggtatgcag gaaaaagtat
     7381 tacagctttt atttgaagat tttacagaga aagaagtgga acagttattt tttggaatta
     7441 tgaaattaag tataggaatt gaaaatgtga aaaattatgt agaaaataat gacgttggag
     7501 aataagcaag tatcaaatca actttgtatg acatcagaga ggggggtaat ttatatgaaa
     7561 agtgtagtag tgatttataa gagtaagtat ggttcaacaa aaaaatatgc taaatggctt
     7621 tcagaattat tgtcttgtga tatttttgaa actaagaata ttaatataga aaaggttatg
     7681 aagtatgata cgattatttt aggaggaggt ctttatgctt gtaatatagc gggaatttct
     7741 ttcttaaaaa aaaattataa agatttgcag aaaaaaaatt tagtagtatt tggcgtagga
     7801 gctgctccat atgatgatga agtaataaag aaattaagag aagtaaactt taaggatgaa
     7861 ttaaacaaag ttccttgctt ttattgtcgt ggggcatggg atgagacaaa aatgtctttt
     7921 atagattata ctctttgtag tttttttaaa aaatctatag ctaaaaaaga acgtcaaggt
     7981 cttaaaccag cagaagcacc tttattgtct ggagaagcct ttgattggtg taataaagaa
     8041 aatttaactc caatacttaa ttatataaga aaactttaag aggtatatat ttataattat
     8101 ttattatcta aag
//
LOCUS       DAGQWR010000171         7772 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.174, whole genome shotgun sequence.
ACCESSION   DAGQWR010000171 DAGQWR010000000
VERSION     DAGQWR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.174"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            113..1171
                     /locus_tag="MIW95_003411"
     CDS             113..1171
                     /locus_tag="MIW95_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBY3152708.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCIFLENCDFNRTIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1275..2459
                     /locus_tag="MIW95_003412"
     CDS             1275..2459
                     /locus_tag="MIW95_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBY3152709.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADGKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNEKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGVIKYYTSSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2666..3379
                     /gene="rpsB"
                     /locus_tag="MIW95_003413"
     CDS             2666..3379
                     /gene="rpsB"
                     /locus_tag="MIW95_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBY3152710.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3458..4369
                     /locus_tag="MIW95_003414"
     CDS             3458..4369
                     /locus_tag="MIW95_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBY3152711.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4454..5158
                     /locus_tag="MIW95_003415"
     CDS             4454..5158
                     /locus_tag="MIW95_003415"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBY3152712.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5184..5741
                     /gene="frr"
                     /locus_tag="MIW95_003416"
     CDS             5184..5741
                     /gene="frr"
                     /locus_tag="MIW95_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBY3152713.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5744..5947
                     /locus_tag="MIW95_003417"
     CDS             5744..5947
                     /locus_tag="MIW95_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152714.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5976..6710
                     /locus_tag="MIW95_003418"
     CDS             5976..6710
                     /locus_tag="MIW95_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBY3152715.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6723..7502
                     /locus_tag="MIW95_003419"
     CDS             6723..7502
                     /locus_tag="MIW95_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBY3152716.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3200 a          794 c         1466 g         2312 t
ORIGIN      
        1 taaaactaag aatctaatat atattttgta actatttaag tatataaaat aaaataatat
       61 ccaaactaat acaaaatagt aataattgtt ttgtactgga ggatatatat ttatgaattg
      121 taaatggata tctaagatag atgagagaaa aaaatgtcat agagaagcag attcttctgg
      181 atattgtatc tttcataaag aaaataagtc tgatgaagag atacagttga tgatggatac
      241 tattcataaa gaagagataa gtgaatttaa tggatttgtt tttgaaaatg aatttaatgc
      301 ggaagaaata ttaacttaca attataaaat actggatttt tctgaatcca tatttaaaca
      361 aaaagcaaat tttaaaaaat atatttttaa aaagaatatt atttttaact atacagaatt
      421 tagagataaa gttttattta atggttgtat attcttagaa aactgtgatt ttaatagaac
      481 catatttagt aaaaactata taaatgaccg tatatttgaa aaagtgaaat ttaaaggacc
      541 tgatttggta gttaataaag tagaaaattt tccacgaatg gatggaataa tttttagcat
      601 gtgtacaaaa tttgtgttaa agaatgttga atatggaaaa agtgagtatg agcatggcaa
      661 gataaattat agaatagcta gaaatcaagc aacaaaaatt ggagaatatg agatgatagg
      721 attttactat tataaggaaa gaatttatag tagtaaaata atgaaacgtt ctaattatcc
      781 tactttcagt gattatttag ttgaaaaatt ttttgaccaa atagcacgtt acacaacagg
      841 ttatggagaa aagccatgga atatattatt agtgattata gcaataataa gtgtttttgc
      901 tttattatac ttatttgtag gcatagaaag ttcaaatagc acacttgtag ctttagatat
      961 aaacaatata ggtgattact cactaagtga gattttcaaa atgtatatgg atttatggta
     1021 ttttagcatg gctacattta gtacagtagg atatggagat atggttgcta caagtttgat
     1081 tgggaaagca ttggcaggta tagaagtatt ttttggagtt acaataggag caatttgggc
     1141 atctgtgatt attaaaagaa tgataaggta atatttgtgt aaatatggat aggtattaat
     1201 tactagatat aaattggacc aaatagggaa aatttaaata taaatatact taataatcca
     1261 agaaggtgaa aaatttgaaa aatgcactaa aaaaaattat atcattagcc atgattctag
     1321 tttttataat accaaattat tcatatgcag atggtaaatc tgtagacaag tattcaaaaa
     1381 catctatact tatagaccaa gaaacaggta gagttttata tagtaaagaa cctgatatga
     1441 aggtaccatt agctagttta agtaagatga tgactttttt acttgcaata gaagctattg
     1501 aaaataaaga agtaaaagaa actgataagg taaaaataga caaatctatt gcatcagtaa
     1561 aagggtcaag ttataaactt aaagatggtg aagaagtgcc acttatagag cttatgagag
     1621 gtcttatgat tgtatcagga aatgatgcgg caattgctat agcaaaacat atttgtaaaa
     1681 cgaaagaagc atttgtagat agaatgaata aaaaagcaaa agaaatagga atgagtaata
     1741 cacattttct aaatccaaat ggtcttccaa tatacgattt aagcaacccc aaaaaaccag
     1801 caaaagaaaa tatctctact gcaaaagata tagctattct tggaaaatac atgtttgacc
     1861 attatgaaaa acaagtaaca gctataactg atatggagac atatacaaat tcagatagaa
     1921 gttttgaaaa aagcaataca aatgcattat taagaattat accagaagta gatggtataa
     1981 aaactggata tactggcaat gctggatatt gcttatcatt ttctatggtt gtaaataaaa
     2041 atgagaaaaa tgagaaaaaa cgtagagtaa taggggttgt attaggtact aaccataaaa
     2101 ataaaagaac ctctgcttca ttagctctac tgaaatatgg aaaagaaaat ttcaatatga
     2161 aaaaagtaat tgataaagat agctttattg gtaagaaata tataaaagga atgaaagagt
     2221 tagaggttac attaaaagca aaagatgaac tttatacaat attaaaagat gatgaaagta
     2281 taaaatcaga agttaagatt aaaaatatag agtaccctgt taaaaaaggt gatactatgg
     2341 gtgttataaa atattatact agttcaggtg acttacttgg aagtgtagat atagtaagta
     2401 ataatagtat agataacgta tcattaaaaa caaaattaaa aatgaaattt gcaaattaaa
     2461 ttcaaaaaat ggtataaaat acacaaaaaa atataaaaat agaaaatatg gaaaaaatat
     2521 tgttatttct tcgcctctgt gatatactac tagaggtgaa aaaataaaat aaatcacaca
     2581 cagcattggg atccttaaaa ggtgcctatt acatggtttt ttagggagaa gatcaagctg
     2641 gaggtataaa accaggaggt aagatatgtc agtaatatca atgaaacaat tattagaagc
     2701 aggtgttcac tttggtcacc aaacaagaag atggaaccct aaaatggcta agtatatatt
     2761 cacagagaga aatggaatat atataatcga cttacaaaaa acagttaaaa aagttgaaga
     2821 agcttacaag ttcactaaag aagtagctga aacaggaaag ccaatcttat tcgtaggaac
     2881 taaaaaacaa gctcaagatg cgataaaaga tgaagctgaa agatgtggaa tgtactttgt
     2941 taatgaaaga tggttaggtg gaatgttaac aaaccacaaa actataaaaa caagaataaa
     3001 taaattaaga gaattagaaa aaatggaaga agaaggcgta tttaatgttc ttcctaaaaa
     3061 agaagttata aaattaagag ctgaaaaaga aaaactagaa aaatacttag gtggaataaa
     3121 agacatgcct gaattaccag gtgcaatgtt cgtagttgac ccaagaaaag aaaacatagc
     3181 tattcaagaa gctcatagat taggtatacc agtagttggt atagttgata ctaactgtga
     3241 cccagaacaa ttagactttg ctataccagg aaatgatgac gctataagag ccgtaaaatt
     3301 aataactggt gctatggcaa cagctgtaat tgaaggtaga caaggtgctg aggaagaagt
     3361 agctgaagac caagaataat aatttgacaa ttaaatggta agggtcgccc ttaccattta
     3421 taatatggaa tagaataagt aggaggaaac ttaaaaaatg gctaatataa ctgctcaaat
     3481 ggtaaaagag ttaagagaaa gtacaggcgc aggaatgatg gactgcaaga aagctttaca
     3541 agaagctgaa ggaaacatgg aaaaagcagt agatttatta agagaaaaag gattatcaaa
     3601 agctgctaaa aaagctggta gagttgctgc tgaaggtcta gttgcaatag aaatgaatga
     3661 tgataataca gttgcttcta tggtagaagt taactcagag acagatttcg ttgctaaaaa
     3721 cgaagacttc aaagtgttcg ttaaagatgc tgcttgtatg gcattagcta ctgataaaga
     3781 agatatagct tctttattag gtgaaactca taaagaggga ataactttac aagaagtttt
     3841 aaataataga gttgcaaaaa taggtgagaa attagacttt agaagatttg ctaaagttgt
     3901 aactaatgga caagttgctg gatacattca cggtggtgga aaaataggtg ttcttgttga
     3961 aatggaaaca gaagctagag atgctaaagt tttagaatta ggtaaagatg tagctatgca
     4021 agttgctgct atgaatccta agtatgtttc aagagatgaa gtagatgctg aatacatagc
     4081 acatgaaaca gaagtattaa ctcaacaagc tttaaatgaa ggaaaaccag ctaatatagt
     4141 tgaaaaaatg gttaaaggaa gattagaaaa agaattaaaa gaggtttgtt tattagagca
     4201 aactttcgtt aaaaatccag atataactgt taaacaatta gttgctgatg ttgctaaggc
     4261 agtaggttct gatataaaag ttgttaaagt tgtgagattc gaagttggtg aaggtataca
     4321 aaagagagaa gaaaactttg cagaagaagt tgctaagcaa cttaagtaat aaccaaagag
     4381 agaacacgca ggtgttctct tttttaaaaa atagaaaaaa ttggatatga aggattgaaa
     4441 taggaggttt ctgatggata aaccaatgta caaaagggta ttgttaaaat taagtggaga
     4501 agcattagca ggagaaagag gttttggtat aaataatgat gtagttaatg atattgctat
     4561 tgctataaaa aaaatacaag aaataggtgt tgaagtagct gtagtagtag gtggaggaaa
     4621 cttctggaga ggcagaacca gtgaaggaat ggatagaact actgctgact atataggaat
     4681 gttagctaca gttatgaatg caatggcgct acaggatgca ctagaaaata tagatgttgc
     4741 tacaagagtt caaactgcta tagacatgag acaaattgca gaaccatata taagaagaag
     4801 agcagttaga cacttagaaa aggaaagagt tgttatattt ggtgcaggaa caggaaaccc
     4861 ttactttaca acagatacaa ctgctgcttt acgtgctgct gagatggaag cagaagttat
     4921 attgctagct aaaaatgttg atgcagtata tgacaaggat ccaaaagtac atgcagatgc
     4981 taagaaattt acagaattaa gttacatgga agttatacag aaagaattaa aagtaatgga
     5041 ttctacagct acatctttat gtatggataa taaaattcca ataaaggtat ttgagcttac
     5101 aacagagaat ataataagag cagtaaaagg cgaaaatata ggaactactg taaaataatg
     5161 aagaatgaag gaggagtaaa attatgaaac tggaaataca taagcaatta gaagagaaaa
     5221 tgaatgggac aattgatgct ttaaagtttg aatttggaac tataagagca ggaagagcta
     5281 atgcacaaat gctagacaaa ataagagttg attattatgg aactccaact ccaataaatc
     5341 aaataggtgc tatatctgta ccagaaccaa gaatattaat gatatctcct tgggataaat
     5401 ctgctatgca tgaaatagaa aaagcgatag ctaactcaga cttaggatta aatccatcaa
     5461 acgatggtga ggttataaga ctttctgtac ctgctttaac agaagaaaga agaaaagaat
     5521 tagctaaaaa agctagtaaa gctgcagaag aatttaaagt tagaataaga aatgaaagaa
     5581 gagacgccaa tgagaaaatt aaaaagatgg aaaaaggcgg agaattaact gaagatgaat
     5641 taaaaaaagc acaagatgaa gttcaaaaaa tgactgataa atttataaaa gaaatagata
     5701 cattattatc taaaaaagaa aaggacataa tggaggtata atattgaaaa aatattataa
     5761 ccttttaggt cttcatgtag atgaggtaaa acaatatttt gatgagcaaa atataaatta
     5821 taccgtaaaa tctattgaag gtaaaaagga taaagaaaaa cttgtagttc ctagagttat
     5881 aaaaatatct gagataggtg atagtgtaga actgataatc acatactttt ctgactcctt
     5941 agtttaagga gtcagaacaa tattggaggc aaaatatgaa taacaacatt atatatgata
     6001 tagacttaaa taacatacct actcatattg ctattataat ggatggaaat ggaagatggg
     6061 caaaagctag atttcttcca agaactgcag gacataaggc tggagtagaa actataagag
     6121 atatagtaaa agaatgctca aaattaggtg ttaagcactt aacattatat gcattttcaa
     6181 cagaaaactg gaaaagaccg aaacttgaag ttgacacact tatgaactta ttatctacat
     6241 acttaagaaa tgaaatagct gaacttcacc aaaacaatgt aaaagtaact gctataggag
     6301 atataagtgc tttaccaaaa acttgtataa gagaattaaa ctcagctaag gaactgacaa
     6361 aaaacaatac aggagttaac ttaaatttag cattaaacta cggaagtaga gcagatataa
     6421 aaaatgctct tatagatata gtaaaaaatt gtgaaagtgg taaaatagat ataaacaata
     6481 ttgatgaaga tattataaaa aactatttaa gcacaaagtc tataccagat ccagacttgg
     6541 taataagaac aagtggtgaa caaagattga gtaacttttt actttgggaa gttgcttatt
     6601 ctgagtttta ctttactgat atacattggc ctgattttaa aaaagaagaa ctacagaagg
     6661 ctatatacgt atatcaaaga agggatagaa gatttggagg acttaagtaa ggagattatt
     6721 ttatgcttac aagaattatt gcttcactag ctctggttcc attatttcta tttgttgtat
     6781 atggaggtat tcctctatat atagctgaga cagcaatagt ttatattgca ttgcatgagt
     6841 tttataaggc atttaaaata aaagatgtac atccaatatt cattatagga tatttatttt
     6901 ctatttattt agctgtaaaa aatattttta atttaccatt agaatatact tatgcagtaa
     6961 tttttatttt atttttagca agtataattt atatgttgat gggcaaaaac aatgttatag
     7021 atgtttcgat tacgttttta ggagtttttt atataggagt atttttagat tttataatta
     7081 taactataaa tggatttgaa aaaggtagta tatatgtatg gcttatattt gtgatatctt
     7141 ttatgacaga tatatttgca tattttagtg gttatttact tggaaaacat aagctaattc
     7201 ctaaagttag tcctaaaaag actatagaag gagctattgg aggaataata ggaagtacac
     7261 tttgttgtat tttatttgga tatttatttg gcattgattt gcttcaatta gcgataatag
     7321 gaagtatagg aagtgtaata gctcaattag gagatttatt tgcatcttct ataaagagat
     7381 atgtagggat aaaggattat ggaaagataa tacctggtca tgggggtata ttagatagat
     7441 ttgatagtgt tatattagtt gcaccttttg tatatagtgc gattaaattt tttattagat
     7501 aaagattggt tggatttaaa agagcaatct ataattgcta gtaatgaaaa tagctatgta
     7561 acttttaaat gcagagatat tctgcagtta ttagttacat ggctattttc attgttcaaa
     7621 aatttagaag accttgggga attttataag aataagtatt actttataat agtatactat
     7681 atttgtagtg agtatatttt ttatactata actatcatta aaaaatattt ttttgtttaa
     7741 taatttcgat atttttagct ttattatata tt
//
LOCUS       DAGQWR010000172         7493 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.175, whole genome shotgun sequence.
ACCESSION   DAGQWR010000172 DAGQWR010000000
VERSION     DAGQWR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.175"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..1838
                     /locus_tag="MIW95_003420"
     CDS             <1..1838
                     /locus_tag="MIW95_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBY3152717.1"
                     /translation="VNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKVLQLEEGI
                     CDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSYIILYFGK
                     IVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFGKNDIEVW
                     GENTGKLFRDDYRGGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTIGIIDNII
                     YSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKEELLGKNS
                     YKISEEEALINKIFESDKGVVENKKVVTFELNVSINDENKCIEITKRPFFDSYGSVIG
                     IIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKEADLIESN
                     DTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEICMSVADF
                     ASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFLYVKDNVF
                     NMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELHEGSISLK
                     SQLGIGSEFIVSLPVRSENNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    1921..2161
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2227..2462
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2547..3764
                     /locus_tag="MIW95_003421"
     CDS             2547..3764
                     /locus_tag="MIW95_003421"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBY3152718.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRSIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            3878..4372
                     /gene="cdeM"
                     /locus_tag="MIW95_003422"
     CDS             3878..4372
                     /gene="cdeM"
                     /locus_tag="MIW95_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBY3152719.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGGNRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            4637..5902
                     /gene="hisD"
                     /locus_tag="MIW95_003423"
     CDS             4637..5902
                     /gene="hisD"
                     /locus_tag="MIW95_003423"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBY3152720.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6105..6857)
                     /locus_tag="MIW95_003424"
     CDS             complement(6105..6857)
                     /locus_tag="MIW95_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY3152721.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANKKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(6975..7244)
                     /locus_tag="MIW95_003425"
     CDS             complement(6975..7244)
                     /locus_tag="MIW95_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152722.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
BASE COUNT         3003 a          782 c         1343 g         2365 t
ORIGIN      
        1 atgtaaattt tataaaaaat atacctgtac cttttttata ttgtagaatt gtaaagagac
       61 aagaagatat agaatatcga gtagaatata taagtaaggg tatgggcaaa gtattacaat
      121 tagaagaagg tatttgtgat aaaaatatac tagatgtatt gcctgtattt aagtctaaaa
      181 aatatttcaa agagttgttc tcaaatgaag tggattgtat aaaaaggtac attccgacac
      241 ttaaaaactg gataaatata aaaaaacaga taatagggga ttcatacata attttgtatt
      301 ttggtaaaat tgtatttgat tatagacaga taatcgattc ctttgataaa aaggaaaaag
      361 tagcttatat taaagacgaa gaaggcatat atatagattg cagtgaaaat ttaataccaa
      421 tattaaataa caacattaaa acaacgaaag atatctttgg taaaaatgat atagaagtat
      481 ggggagagaa tacaggaaaa ttatttagag atgattatag aggaggagta tctagtaaaa
      541 aaagattttt acaaaatctt tttgaatatg aagaaacatt ttttatggtt gaaaaatatt
      601 ttttgtatga tgaagacgaa cttttaggta ctatagggat aatagataat ataatatata
      661 gtggttacag taatagaaat tataactcaa aagatttaat gaagatgata gaacactcaa
      721 ttccagagaa tatgttttat aaagatgtgt atggaaatta tattggattt aattcaggtt
      781 ttttgaattt agcttgtatg aataaagaag aattattagg gaagaatagt tataaaatat
      841 cagaagaaga agctttaata aataagatat ttgaaagtga taagggtgta gttgaaaata
      901 aaaaagtagt tacatttgag ttaaatgtaa gtataaatga tgaaaataag tgtattgaga
      961 ttacaaaaag gccgtttttt gatagctatg gaagtgttat tggaataatt ggaacagcaa
     1021 gggatataag tagaagaaaa agattagaag aggaaatgga taaaactagg atggaatttt
     1081 ttgcaaattt atcccatgaa cttagaacgc ctataaattt aatatcatct tcattacaag
     1141 tgatagaaaa aaaagaagca gatttaatag aatcaaatga tactttaaaa agaaatttag
     1201 gtataataaa acaaaatgga aatagaatac tgaggttagt aaataatgtt atagacttta
     1261 caaaaatgca atcaggatat ttagatttta aaccagaaga atctgatata attgcattta
     1321 tagaggagat atgtatgtct gtagctgatt ttgcaagcca aaataatata caacttacat
     1381 ttgatacaga aatagaagaa ttttcaatgt tatttgattc agaaaaattg gaaagaataa
     1441 tattaaattt actttctaat ggaattaagt acaataagaa agatggaaaa ataaatatat
     1501 ttttatatgt gaaagataat gtatttaata tgaaaatatc tgatagtgga ataggtatac
     1561 caaaagaaaa aatagataaa atatttaata gatttgaaca gattgacaat gaactttcct
     1621 acagagttaa aggaagtggt attggacttt cattagtgaa atctttggtt gaattgcatg
     1681 agggaagtat atctttaaaa agtcaacttg gtattggaag tgaatttata gtttcattac
     1741 ctgttagaag tgaaaataat attgaaaaat ataatcataa aagagaaatc agcaatgaac
     1801 taagtaaaaa attagaaata gagttttcgg atttataagt ttaaatataa aaaataaaat
     1861 tttaaaaaag tattgacaat tgatttagaa gggtttaaaa taaattacaa cataataaat
     1921 aaaaaggcga tgaataggaa gagtaaatct aattcgatta ccagagagaa gagtccattg
     1981 gctggaagac tttttaaatt agagggattg aaaactacct aggagctact ttctgaagtc
     2041 atctgcatta gtagatttta gtaagaatag tcgttagctg cgttaaagtg taaagtgagt
     2101 tttcatgata tatgaaaact aaattaggtg gaaccgcggg aataatactc tcgtccttat
     2161 gttataagga tgagagtttt ttgttgttta aaaatataaa cagtaatcat aataattata
     2221 gaaaatataa aagcgatgaa taggaagagt aaatttaatt cgactatcag agaggaaagt
     2281 tcacaggctg aaagactttc taagtttgag aaattgaaaa ctacctagga gctgtggtct
     2341 gaagttactt taaaaagttt tagtaagact aaccgttaac tgcgttaaag tgtaaagtgg
     2401 attttatcaa taaatgataa aatcaaattg ggtggaaccg cgagtataga ctcgtccctt
     2461 tatataggga tgagtctatt tttattgcaa taaaagattg aaaataaaaa aaaatcggaa
     2521 aattataaat ttaggaggaa aataaaatgg aaaataaagt gaaaattgga gtactaggat
     2581 atggagttgt aggaagtgga cttatagata taatagataa taataaagaa aaaagaagta
     2641 tagaaatagt tggaatttta gttaataatt tggaaaaaca taaggataaa aaatattcta
     2701 atataataac taataatata gatgatatat ttaataaaga tatagatata cttgttgaag
     2761 tcatgggtgg tttagaacca tcattatctt atataaaaaa agcattaaac aataaaattc
     2821 atgttgttac agcaaataaa gatttgttag ctgaatgtgg agatgagctt gcaaaattag
     2881 caagtgaaaa taaagtaagt attaaatttg aagcttcagt cgcaggaggt attccagtat
     2941 taaaaccaat aatagaatca ctagaaggaa ataatataga tagtataaat gcaattttaa
     3001 atggaactac taattttata ttatctaaaa tgtatgatga gaatctatct tatgatatgg
     3061 cattaagaca agctcaagaa ttaggttttg ctgaagcaaa tccagagtca gatgtattag
     3121 ggtatgatgc tgctagaaaa ctatcaatat tatcaactct agcttatgac aatagagtat
     3181 attggaagga tgtgtattta gaaggaatta cagatataga tgaaaaagat attgaatatg
     3241 caaaaaaatt aaattgtaaa ataaaattga ttggtcaaag taagtatgaa aatgataaag
     3301 taagtgcttt tgttagacct gttttagtag aaaaagataa tatccttgct agaatagaca
     3361 atgagttcaa tgctgtcata gtgaatggag attcagtagg agaagtttct tttgtaggta
     3421 aaggagctgg aagtttagca acaggaagtg cggtttattc agatgttata gatataatag
     3481 ataatagagt ttcaagtata gattctttta ctaaagataa aatacaagtt aataaaatag
     3541 ttagagaaaa gtgtggagca cttcttagat ttaaaaaatg taataaagat gaaattctaa
     3601 atatagttga gaattgttta gtaaaattcg atattttaaa tgatgatgat gaacttgcta
     3661 ttatggttta tgctgattct gaatatgaaa taaacaacag tttatgtcta ataaaagata
     3721 aaggatattg cgaaaaaatg aataaaatgt tgaaaataag ttaaaaaaga aaaaataagt
     3781 ttatatgtaa catattttga cgattttaat actatatagt atgagatgaa acaaaacatc
     3841 tcataaaaaa ttaaaattca agggagagat atgttatatg gaaaataaaa aatatgcaaa
     3901 tggtggttat tcagaagact ggtatgaaag aggagaatct acagctaaat ggttccagaa
     3961 tgatagagaa gaatatgaaa gagcagcata cgatgaagat agagaaagaa gaggctcaaa
     4021 ctgtggatgt tcagattcag gaggaaatag acctagaaac tgtgaaagat ttagaagaga
     4081 agctgagata agagaaagag aagcaagaga agcattctgt gaatcttcag agagaaagaa
     4141 agaagcacta gcatatgaat gtgaagctag aaaattatgg gaagaagcag aaaaatactg
     4201 ggatgaatat tcaaaataca actataaagg aatcgaatat ttagcagaag ctgctagatt
     4261 atttgatgaa ggtatggaat gtgaagctag aagaaatgga aataatggag gaaacaataa
     4321 taattgttgc cataaatgcc ataaatgtaa ttgtaactgc tgtagaaaat aaaaaaattc
     4381 attgacaaat caatagttaa aatatatcat attatgtata ataaagagaa aattcagaaa
     4441 aaaataatga ttagaataaa tttaagtata tttattagac aaatagtgct cagttagtga
     4501 ggtgagtaca atatgattaa actcccatcc tctagctttt gatagagggt gggagttttt
     4561 atttattgat atatcttaaa tgaggataat attgtaaaat aaatgagtat gagattgtaa
     4621 aattaggagg taaaaaatga aaagtattaa aacatcaagt atgaaagaaa aggtagatga
     4681 aaagatatta agagagcaag taagttctat aatagaagat ataagaaata ataaggatgt
     4741 tgcacttaaa aaatataatg agaagtttga tagaaataca agagatgaat ttagaattac
     4801 taaggaagaa ataaaggaag cttataagca tgtagatgac gaatttataa ataacttaaa
     4861 gattgctgcc aaaaatataa gagaatttgc taaagctcaa aaactaagtt ttgaaaaccc
     4921 ttttgaaaaa gaaatttatc caggtgtaat cttaggtcaa actaatattc caatagagtc
     4981 atgtcttgca tatgttccag gaggtcagta tccattgttt tcaacagctc ttatgcttat
     5041 aatacctgca aaagtagcag gagtaaaaag aatagtggca tgttcaccaa ctatgaaaaa
     5101 tactgaaaag ataaatccta aaacattggt agctatggat atagcaggtg ctgatgaaat
     5161 atatgcaact ggaggagttc aagctatagc agcatttaca tatggaacag aaaagattaa
     5221 tccagttgac ataattgtag gaccaggaaa taagtttgta actgaagcta aaagacaatg
     5281 ttatggacaa gtaggaattg attttgtcgc tgggccaagt gaagttctta taatagcgga
     5341 tgaaacttca aatccagttt atattgcagc tgatttatta gcacaatgtg agcatgattt
     5401 aaatgcaaga ggaatattat tgacaaatag cttggaagta gcacaaaaag ttgaaaaaaa
     5461 tatagaaagt atgctaaaag atttgccaac aaaggacata gcatatagtt catgggaaaa
     5521 taatggagag ataatattgg ttgatgatat ggaagaagcc attaaaataa gtaattttta
     5581 tgcaccagaa catttagaag ttgcagtaaa tgagtataat gatatttgtg atagattaac
     5641 taactatggt tctttattta ttggaaatct ttctgctgag gtgtttggtg actatgtttc
     5701 aggtaccaat catacattac caacacttaa agcatcaaga tatactggtg gtgtttgggt
     5761 tggaactttt atcaaaactt gtacaaagca gatatttaat gaaaaagcta tacaatcatt
     5821 agcacctgta gctgaaaagc tggcaaaaga agagggatta tatgcacatg caaaggctgc
     5881 tgaggttagg tttaaaaagt agtaaaaaat tttattttgg cagtcgagat ttagtattag
     5941 actgagataa agaaaacgct gagaacctga ataaatacta tattttgtgg gcctcggcgt
     6001 ttcttttata tctgtatata ataggataat actacctgtg tgtttaaatg ctaacttaat
     6061 atttcttttc taaataatgt ttatgaaata gttaatattt ttagttaata cttgaaaaaa
     6121 acttttgcta actgtgcaaa ttcatctgca tttgtcaata tagcagggtg tcctccctga
     6181 ctaaaaatgt gaatacttgc ttttgaaata aaagttgcca tttgcttgta ctcttcttca
     6241 agattatgac gacacatttc gtcttcctta cttcctgtaa ataaaattgg catttctaat
     6301 ttacacaatt ctttatgaaa taaagggcgt ttcttgtttg cacattgcaa tagagctttt
     6361 gtatcaagat taacaacatt ctcccaatct tttccatgac accattcata gaattttcgt
     6421 gcttgaatat cttgctttga tttttccctt ccagtgatta gattatctat gaagttatca
     6481 ttaagagtac gtccatcaaa gctatctgct ataattgctt caactaaatc gggacgttct
     6541 aatccagtat taacagctgc ccatgctcct ccacttgttc caattaagcc aactttacca
     6601 caattcaaat gttcaatcag tgctattgtc tgtaaggctt catcatgcca tatgtcagga
     6661 gaaaattgag aaactctatc agactgtcca ttacctaaaa aatcaattaa gatacatcta
     6721 aaattttcta catataatgg cacaagtgct ttaaacatat tagaagaacc agtattacca
     6781 tgcaggaaaa ttatcggttt accttgtcca tattctttat aaaaacaaga attaccttga
     6841 tactgaaaat atgacatata ttttcctcct ttttatttgt taagttaatt ataacaatat
     6901 aggtatgaat gaaaaacctc atgttctttc ttagaataaa aatgtgccac gcataaaacg
     6961 tgacacattt ctaattataa attttgctgt ttaagtattc tttgtatgct tttctctgtc
     7021 aaaaagtatt tctctgataa ctgcttgata ctcattccat tttggaaatt tatacagatt
     7081 tgagaattac gagaagctaa gacttgtttg gtgtttgtgt tttcacccca atgctttttg
     7141 tgctcttgct ttttgggaat ataaatcact ttaccatcta tatattcttg cacttgctca
     7201 atcagttctt taggcagtaa gtcaatcgaa ttttgatagc tcattttgca cctcctaaat
     7261 aattactctt taggattagc aaaggctact gctactcaca attaaaaata aaatttttta
     7321 ttatgtacaa tagcctttgc tatgcacaag ttccaattcc cataaactaa atactccttt
     7381 ctggtttgaa aaattcatct gaacattgat ttcgatttta agatagagtt caatgacaaa
     7441 tttacatttt tattatatat attcagatag ataattgcaa ctgtctttgt atg
//
LOCUS       DAGQWR010000173         7410 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.176, whole genome shotgun sequence.
ACCESSION   DAGQWR010000173 DAGQWR010000000
VERSION     DAGQWR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.176"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            40..1017
                     /locus_tag="MIW95_003426"
     CDS             40..1017
                     /locus_tag="MIW95_003426"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBY3152723.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKTHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1010..1978
                     /locus_tag="MIW95_003427"
     CDS             1010..1978
                     /locus_tag="MIW95_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBY3152724.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2162..2752
                     /locus_tag="MIW95_003428"
     CDS             2162..2752
                     /locus_tag="MIW95_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBY3152725.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2771..3541
                     /locus_tag="MIW95_003429"
     CDS             2771..3541
                     /locus_tag="MIW95_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBY3152726.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3678..4646
                     /gene="dusB"
                     /locus_tag="MIW95_003430"
     CDS             3678..4646
                     /gene="dusB"
                     /locus_tag="MIW95_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBY3152727.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4755..5234
                     /gene="greA"
                     /locus_tag="MIW95_003431"
     CDS             4755..5234
                     /gene="greA"
                     /locus_tag="MIW95_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBY3152728.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5249..6778
                     /gene="lysS"
                     /locus_tag="MIW95_003432"
     CDS             5249..6778
                     /gene="lysS"
                     /locus_tag="MIW95_003432"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBY3152729.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7087..7281
                     /locus_tag="MIW95_003433"
     CDS             7087..7281
                     /locus_tag="MIW95_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152730.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NIWADRVRANSYSYENKIAK"
BASE COUNT         2953 a          753 c         1504 g         2200 t
ORIGIN      
        1 aaaataagtc atgaaattat gtcatattag aggtgaaata tgcgagataa aataatagag
       61 attatattaa ataataaatc tgaatttatt tcaggagaag aattatcaaa acaattaggg
      121 gtgtcacgtg cagctatatg gaaacatatg aaagccttaa aagaagaggg atataatata
      181 gaatctgtaa ataaaaaagg gtatcgatta gcggaaaatc caactgattt attaagccca
      241 cagaatattt actataaatt agatacagag tttattggaa aaaatattga acatcttgac
      301 actatagatt caacaaatga acatgctaaa aggatagcta taaatagtgt agatgggact
      361 gtgattttaa gtgaacatca agatatgggg aaaggacgat tgggaagaag atgggattca
      421 aaaactcatg aaggaatctg gatgagtatt atattaaaac cagatatgga accattcaaa
      481 gctccattta taactttaat agcaggagca agtgtaacga aagctttaaa caatctagga
      541 gcaaatacat taataaaatg gccaaatgat gttattttaa atggaaaaaa aatatgtggt
      601 atattaacag agttatctgc tgaaattgaa agaacaaatt atgttgttct tggaattgga
      661 ataaatgtta aaataatgga ttttgaacaa gaaatagctg acaaagccac atcattgtat
      721 aaagaagggt atatgttatc cagagtagat attgttagga atgtattgac tgaatttgaa
      781 aaattatatt tagattatac tgtaaataat tccaaagaaa aaaccttaaa aatatgtaga
      841 gattattctg caattataga taaaaatata tatgtattaa aaggagataa tagagaacta
      901 gtgaaatgtt tagacataaa tgaagaggga aatttattag ttcaaagaag tgatggaagt
      961 ataaaagaaa ttatttcagg tgaagtttct ataagaggag aaaaaggata tgtataataa
     1021 tattttggat atagaaggct taaaggttgg gcaggttgag gatagaaatg gtcttactgg
     1081 gtgcacagta gtaatatgtg aagaaggagc tacttgcggt gttgatgtaa ggggttctgc
     1141 tccagggact agagaaaccg atttattaga ccctgtaaat atggttcaaa aagttcatgc
     1201 agtagttttg gctggaggtt ctgcatatgg tcttgaatcc acttgtggtg taagtagata
     1261 cttagaagaa aaaggaattg gctttgatgt aggtgtaggt aaagtgccta tagttgtagg
     1321 agcagtttta tttgatttag gagtagggag ctttaaatgt agaccagatt taaagatggg
     1381 ttatagagct tgtgaagttg caaatactga tattttaaat caaggaaatt atggagcagg
     1441 ttgtggagcc actgttggaa aaataagagg tcaggaatat gctatgaaag gcggaatagg
     1501 tagctattct ataaaattag ataatggtct aattgtatct gcattaattg ctgtaaatgc
     1561 tcttggagat gtttatgaaa atggtaaggt tatagctggt gctttaaatg ataaaaagac
     1621 agaaacaatt gatacatata aattaatgaa acaaggtgaa agtaaaggtg gatttagtgt
     1681 agataatact acaattggga taattgcaac aaatgctaag ttaaccaaag ctggatgtaa
     1741 aaagatatct caaatgggac atgatggata tgctaagtct atttttccta tccatacacc
     1801 tcatgatgga gatacaatat tcacaatatc aacaggagaa atagaagcag atataacatt
     1861 agtaggttca ttggctgtag aggttgtgga aaaaagtata ataaatgcag taaaaaaagc
     1921 ggatagtgta gaaaatataa tggcatataa agatatagta aaaaaacgag aaatatgatg
     1981 aaattagcat atttttcttg tcaaattgtt gacaatggca actcatcttg ataaaattat
     2041 tcttgttaga gcgggcatcc tacaatggta gcttgactca tagttataag aaaaaaacga
     2101 aaaaaattaa aaaattaagt agtccggcat cttaatgagc tggaacggag gtgtattttt
     2161 tatgatgaac acaagtgcat cagtaagaaa agtaaatgta agaaagatga ctataatagg
     2221 agtactatca gctatttcta ttatgctatc tatgactcca ttgggattta ttccagttgg
     2281 acctacaaaa gcaactataa tgcacatacc agttattatt ggagctatta tggaaggacc
     2341 aatagttggg gcagcaatag gatttatatt tgggatatca agtctcttaa atgctataac
     2401 aaacccaaca gtaacatctt ttgtttttat aaatccatta gtttcaatat taccaagagt
     2461 tatgataggt gtattagctt attatgtata tcagttaata attaaggcta caaataaggt
     2521 atacatatca ggtttaatta caggggcaat aggttctctg ttaaatacag ctggagtttt
     2581 aggaatgata tatgtcttat atgctgacaa gtatcttcat gcaatgggta aaagtggttc
     2641 agcaggaaaa gtaataatgg ctcttgccgc agcaaatggg gtgccagaag caatagtagg
     2701 ggctttagta gtagctgctg ttgccactgt acttaagaaa agcaaaaaat agaaagaagg
     2761 ttaatgacat atgcttctag tatttgatgt tggaaatact aatatggttt taggtatata
     2821 taaaggtgac aaattagtta attactggag aattaaaaca gatagggaaa aaacgtctga
     2881 tgaatatgga atcctgataa gtaacctatt tgattatgat aatgtgaata taagtgatat
     2941 tgatgatgtt ataatatcat ctgtagttcc aaatgtcatg cattctcttg aaaacttttg
     3001 tataaagtac tgtaaaaaac agccattaat agtaggtcca ggcataaaaa caggtctaaa
     3061 tataaaatat gataatccaa aacaagttgg ggcagataga atagttaatg ctgtagcagg
     3121 gatagaaaag tatggagcac caagtatact tgttgatttt ggaacagcaa ctacattttg
     3181 tgctatctct gaaaaaggtg aatatttggg tggaacaata gcaccaggaa taaaaatatc
     3241 tagtgaggcg ttatttcaaa gtgcgtctaa attacctaga gtagaattag ctaagccagg
     3301 tatgactatt tgtaagagta ctgtatcagc catgcaatct ggaataattt atggatatgt
     3361 tggtttagtt gacaaaataa taagtatgat gaagaaagaa ttgaattgtg atgatgttaa
     3421 ggttatagct acaggtggat tagctaaact gattgcttca gagacgaaaa gtatagatta
     3481 tgtagatggt tttttaacac tagaaggatt gagaataata tatgaaaaaa accaagaata
     3541 attaatatac agggaattgg gttatatatc caattccctt tttatgatag atttctagct
     3601 atatgtggta tattataatg aggaaataag aatttaaagt taaatcacaa aataaaggca
     3661 aaaataggag aaaaacaatg aaaataggaa atttagaatt gaaaaataaa gtttttctat
     3721 cacctatggc aggtgtaaca gaccttccat ttagattaat ttgtaaagaa cagggatgtg
     3781 ggcttttata tacagaaatg ataaatggta aggcactttg ctatgatgat gaaaatacta
     3841 aaaaaatgct taagatagaa gaggaagaac atcctgtagc agttcagata ttcggttcag
     3901 aaccagagtt tatgggaaga gcagcagaga taatgaatga ttattcaaat gagattctcg
     3961 atataaatat ggggtgtcca gctccaaaag tagttaaaaa tggagatggt tcagcactta
     4021 tgaaaaatcc taagcttgca gaagaggtat tgagggcagt agtgaagaac tcaaaaaagc
     4081 cagtgacttt aaagattcga aaaggctggg atgataatag tgtaaatgct gttgaaatag
     4141 caaagatagc agaggattgt ggaataagcg ctttagcaat acatggaaga actagagaac
     4201 agttttatac aggcaaagca gactgggata taattgcaga gattaagaaa aatttgagta
     4261 tacctgttat aggaaatggt gatgtattta caatagaaga ttcaataaat atgttagaca
     4321 aaactggttg tgatgctata atgataggta ggggtgctca aggtaaccct tggatattta
     4381 aaagaataaa ccattatatg aatacaggcg agattttgcc agagccaact cttaacgaaa
     4441 aaataagtac tgccattaag catttaaaac ttgcagtaga agaacatgga gagtatgtag
     4501 ctgtaaggga aatgagaaaa cacatcgctt ggtatttaaa aggacttaga aactctgcaa
     4561 aattaaggga tgaaattaac aaaatagaag actatcaaga agttgtgtcg aaattggagt
     4621 actatatgga agactccttg acataaaaat aatagtgact tataatatag cgcataaatt
     4681 aaaaaaagat tataaaattt ttaaaatata attaggaaaa ttcctaagaa atttgtgaag
     4741 gagttgtatt tataatggaa gaaaataaag agtttttatt gacacaagag ggatatgata
     4801 aattagaaga agaattagaa aacttaaagg ttgtaaagag aaaagaagtt gcagagagaa
     4861 taaaagttgc tatatctttt ggtgatttat ctgaaaatgc tgagtatgat gaagctaaaa
     4921 aggaacaagc acaagttgaa gaaagaattt taaaattaga aaatatggtt agaaaagctg
     4981 ttatcataga tgaaagcaaa atagacctta atgttgttac aataggatca atagttaaag
     5041 ttaaggattt agaatttgat gaagaggttg aatatactat agttggttcg acagaagcag
     5101 acccatatga tggtaaaata tcaaacgaat ctcctgtagg gaaagcactt ttaggtagag
     5161 ctgcgaaaga agttgtggaa gtgcaagttc ctgatggagt tgctaagttt gaaatattag
     5221 aaataagaag ataatttgga ggaaaaaaat gaaaaataat caacaaagca atgaagaagc
     5281 gcaaattcaa gaagacctta gtgaagtact tcaagtaaga agagataaac taaaaaaatt
     5341 acaagagtca ggtagagatc cttttaaaga aagtagatat gatagaactc attattctat
     5401 ggatataaaa gataactttg attctcttga aggtaaaact acaaaaatag caggacgtat
     5461 aatgagcaag agaatacaag ggaaagctgg ctttatagat attcaagacc aagagggaag
     5521 aatccaatct tatgtaagac tagatgctat aggagaggaa gaatatagtg tattttcaac
     5581 atatgatatt ggagatatag ttggaattga aggagaaatc tttaaaacta aaaaaggtga
     5641 aatatctgta aaggctaaat ctgtagtatt actatgtaaa tctttacaag ttttaccaga
     5701 aaaatatcat ggattaaaag accaagaact aagatataga caaagatatg ttgacttaat
     5761 agtaaatcca gaagtaaaaa atgctttttt aattagaaca aaagcattaa aagcattaag
     5821 agcatactta gatgatagag gatttttaga agttgagact ccaatattaa atactatagc
     5881 aggtggggca aatgctagac catttataac taatcataat actttacata taccaatgta
     5941 tttaagaata gcaaatgaat tgtatttaaa aagacttatt gttggtggat ttgataaagt
     6001 ttatgaaatg ggaagaatgt ttagaaatga aggtatggat ttaaaacata atcctgaata
     6061 tactgcaata gaattatatc aagcatatgc agactatact gatatgatgg aaataacaga
     6121 aaatgttatt gcacatatgg ctgaagttgc tacaggaagt atgatagtta actatcaagg
     6181 aacagaaata aactttacac caccttggaa aagaatgagt atggaagatt gtgttaaaga
     6241 atattctggt gtagattttt ctactataaa tacagatgaa gaggcactag aagttgctag
     6301 agaaaaaggt atagagataa aacctggaat gagaagagga gaagtaataa atgctttctt
     6361 tgaagaattt ggagaagata aattaatcca accaacattt ataactcatc atccagttga
     6421 agtatctcca ttatctaaga gaaatgttga agacccaaga agaactgata gatttgaggc
     6481 atttgctaat aagtgggagt tagctaatgc attttctgaa cttaatgacc ctattgacca
     6541 aaaaggaaga tttatagacc aattgagaaa aagagaatta ggtgatgatg aagcttttga
     6601 aatggatgaa gatttcttaa aagcattaga ggtaggtcta cctcctacag gaggactagg
     6661 aataggtata gatagagtaa ttatgttatt aactaactct ccatctataa gagatgtatt
     6721 attattcccg actatgaaac ctattgataa caatccaaat aaagaagaag aaaactaaga
     6781 aatattagca ttctactaag ttttaaataa agtttagcta ttaaattagc aataaatagc
     6841 taaattttgt ttaaaatgtc agtgtagaat gctttttata tgcttcaaag attcttttag
     6901 atatttaatt tataaattac caacaaacgt tagaaattga tattagtttt tgttgattga
     6961 gactttatat ttagaaaata tgattgaaag taatttatta tgaaaatatt tagaataatt
     7021 tatgagaagg taaaaagaat aatttatgag aatactatat ataaaaaatt attgggggaa
     7081 tgtctgatgt ttcaagtaat taatttttta tgtttatttt gtgttggatt tttggttctt
     7141 gatgttatga tatatttttg taaggatgca atggatattt ttgagggtac aactaatgtt
     7201 aataaagtta agagtataaa tatttgggca gatagagtga gagctaattc atattcttat
     7261 gaaaataaaa ttgcaaaata ggtaaaatta tatatgcatg tattgtttgt aatatcaagg
     7321 gttatatcta ttagttttaa ttatatttat taaaaaaacc aaaagttata acctttttta
     7381 aaataaaaaa caaaaaaagt attgacgaaa
//
LOCUS       DAGQWR010000174         7299 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.177, whole genome shotgun sequence.
ACCESSION   DAGQWR010000174 DAGQWR010000000
VERSION     DAGQWR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.177"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(108..497)
                     /locus_tag="MIW95_003434"
     CDS             complement(108..497)
                     /locus_tag="MIW95_003434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBY3152731.1"
                     /translation="MRAVGLSSVCRKKKFSYVKCTPEVIAENVLSRKFSADKTSQKWL
                     TGVTEFKLTNGMKAYLSAILDLDDRSIVSYVIGKSNNNNLVLETFDKAIELYPNAKPI
                     FHNDKSFQYTSKVFKSKLLTQGMIQSM"
     gene            complement(509..969)
                     /locus_tag="MIW95_003435"
                     /pseudo
     CDS             complement(509..969)
                     /locus_tag="MIW95_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1398..2408
                     /locus_tag="MIW95_003436"
     CDS             1398..2408
                     /locus_tag="MIW95_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152732.1"
                     /translation="MITKIKKTYIYLKEIQKIYKINEHKELVHIIKKLINDNKIKPIK
                     TSDLTPQHEQLYTKYRIIKQETEEDKKILEEINYKICDKLSIDYYRKNLVHYKNNRVA
                     IMDLNSYLIKKSDNLSKKISINERSYEIFKDEKFISSKVGKEVLKNLKIDLIKDLNVY
                     KTPEPFIYLSINRISPQKILIIENKDTYITITKMLLEGKEILKNKIDTVIYGEGKKII
                     NSIKGLKEDITIEYLTNKENEFLYWGDIDKTGLSIYTSLKDTNEINNIRLFEVAYEHM
                     IDKTKENTLRNIPKNQKDNFECGLENINNLNLKESIKNILVQGKYIPQEALNRFDLEE
                     YN"
     gene            2408..4027
                     /locus_tag="MIW95_003437"
     CDS             2408..4027
                     /locus_tag="MIW95_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152733.1"
                     /translation="MEMINILEGYNKRVNCIGSYYLIATLANNRNKFKEFDNIQFYNL
                     LIQVLCYIFDRSLRRKNCLRDDIKDFIEEINRMDYKIMLSEDDLKDLANYIINGLTNS
                     GKVYLFTYYSLEQEKHIDESIKIIEDKNVKINNQERLSYSLTTEGYRLLLSTKEYDEL
                     FQIQISQMIAKLRIEKGDYQGAKEDIYDIINTLEIHQQKIDNYIKIIKVNILYIKEEK
                     YSTVIDTTVETLLEEMDKYEELKKEVKLLIKDKEDYLEKDEDIDKEKIKNIRTQLLQM
                     KELIDGIVLAKSTATNLLSRVQDFREEFKEILKKLLTTPVIKRFNFKEEILDRLEKDS
                     DFLLIIRELYISLFKVELPNRFNIGVAYQEQKLLNTREIENEIVNKDELEIIENKEKE
                     ERINYCENYYFDFFKYLIKYGLEKGQFTLEEFLKNYLKYDKVIYLEITKDSKLMRDII
                     IYFANQRESINIKKIIRMLENSAFSPNTDFQIERLIERLLVEDFELLNSVSHIKSNKI
                     FEDEMKINTEINLETMTAVKLEIANIKFEII"
     gene            4045..4773
                     /locus_tag="MIW95_003438"
     CDS             4045..4773
                     /locus_tag="MIW95_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152734.1"
                     /translation="MNISLNDYKIANEIFYRLLKKSELKEKEAKELFLAYINNSNVRY
                     ALEAICEVSKIKIILISDTLYLIPNNDNDIFGFDYRRESLLGKDLKDSYLGYLIMTII
                     FSEFTNGVAPASYIQVLNLLDLVQESLERSIARDNLEEIEKSCEFNITILKNYWESKR
                     KWDESSKKGIETTSADFQIGFIRKIINFLKNEGLIVHIEDEDKIKPTRRFLDLMNNYF
                     LDEERKVLIEKLVLRKGESINASN"
     gene            4760..>7299
                     /locus_tag="MIW95_003439"
     CDS             4760..>7299
                     /locus_tag="MIW95_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152735.1"
                     /translation="MPVINKYKIINFKFGMGQERILSNRTMELYGENLQVEAENTAGK
                     TMSIQTIIQSICPLSDVAKPFTAIYNKKEPLYSMTEWLLDDGKTLLLTGIGFEKKAGS
                     SKEEGKEKKDDLYKYFMFIIEESKEMEVDIDNIPLLKNNDKGIRIVQPLTASQDYIKM
                     LRDKYGKNKVHFFNNYLRKQHREKLEEYGINQSEWKEIIIKLNSNNKEGGLSDFVKEH
                     NTSEKLIRDKILPLIETSLTTEEFAPILQIKEQVRKCINNIIDMNEKLSDYKNYRDLN
                     IRVESLQNKINRILFIEEDKNNVLGELANLYVHIENKRNELNEEIKQYQKMKASKEEE
                     FKIVEYEEDSYEYFRKEKEIQDTQVEFDKAKNELNDKIEELELDEQYLNISKFKRKKK
                     GLEEGEKDKIEKESEKQRKEVNQEEITAIINRLGSSIHYCLKEESIINNEKLKENTNN
                     NIAIQEKKNQLNDDKSKLTKVLISKNSEIKYIEGKERDFNNQLHEFKLNNTDIKGFIS
                     NNFLGEVIDYTRYINYLNIKVKSIEENLESKVMQIEESNKLKDDINEAINNLKLDNHE
                     SYMKLQVLKTRKKDIDNFKEKYKIIKTRFEIDNELDNSTKLYKEVKQELNKMKLQVQD
                     LENNKKQLEKELEDLQNYKDIIFEEEILTDLERNGIKPIEGMKYLLTLDGDLEYKNNL
                     ISKNPLLPYSIIITNKEYQKLKQINLKNKTKISMPIFVRDNIEDTFITTSSNLVISNG
                     LNILSSFDNKALDPEKREKDMNLIKEQINSIRDKVEQYKAEIEILEEFKLLTNKIDFT
                     AEDLSIDVKIENYKKIIFEINNKIKSNENNEKNLSNKIKELYENKEKL"
BASE COUNT         3290 a          782 c         1019 g         2208 t
ORIGIN      
        1 tttgactaat tgttcgtatg tatcaagttt tcttaaatag tacatttcca cttttaaaat
       61 tctccaaaat ccttccatag gagcattatc aatacaacga gcaactctta catactttgt
      121 atcatgcctt gtgttaaaag ttttgattta aataccttac tagtatactg gaaactttta
      181 tcattatgaa aaataggttt tgcatttgga tatagctcta ttgctttatc aaaagtttct
      241 aacaccaagt tattgttatt tgactttcca ataacatatg aaactatact tctatcgtct
      301 aaatctagaa ttgcgcttaa ataggccttc atcccattgg ttaatttaaa ttcagttaca
      361 cctgttaacc atttttgaga ggttttatct gcactaaatt ttctacttaa tacattttcc
      421 gcgatcactt ctggcgtaca tttaacataa ctaaatttct tttttctgca aacagaactt
      481 aagcctacgg ctctcattag tctgtacact ctctacaatc tttaacaatt tcaactcttt
      541 catctaatgc tgtttttcta gtctccataa agatttctcc cttcgaggtt tcttttatct
      601 acttatgagg attatactct ttaccccaag cttttagtga ataaactgat ctcattttat
      661 atttttttga gattgtgtca taagatcctt caccatttag ataagatttt atagcattca
      721 atttaaattc ttcacaatag cataatttac aactttgttt tattaaacca tcatttccaa
      781 aagcttcata atttcgcatc cactctctga atgtacgttt atccaatatt taattcttcc
      841 attattttta ttactgtatt ctcacctttt aaatatgatt caattgcttc gatttttttg
      901 ctctgaagat atcttagttt tagatagtct tacatatttt aactgtctcc taagatgagt
      961 ataattcatt catcatcagt tctatagaat acacctagtt cctacttact gtaggaacta
     1021 ggactatatg aaacccattc atagattgta gtttcttctg ttgcattata gcgcagaata
     1081 gtaaattcat gtgaaatttt attttgaatt tactatttat atataaatga agagtggtct
     1141 tattttcaat taaaatttgg aacaattttg ggttgacatc tacaattaag gatttaagtt
     1201 tttgttatac taataaataa aaacagagat tcaaaatata taaataatac ttaaactagt
     1261 gctgatagat tttctttaag ttctagtttt tttattgtca ttatctaata tgtactattt
     1321 tatgtacagt caatataatc tgaaattgta aaatggtgta taatatagtt ataagtaaag
     1381 aagggattta aacatgtatg attacaaaaa taaaaaagac ttatatatac cttaaggaaa
     1441 tacaaaaaat atacaaaatc aatgagcata aagaactagt acacataata aaaaaactaa
     1501 taaatgataa taaaataaaa ccaataaaaa cctcagatct aacacctcag catgagcaat
     1561 tatatacaaa gtatagaata ataaaacaag aaacagaaga agataaaaaa atattagaag
     1621 aaataaatta caaaatatgt gataaattaa gtatagatta ttatagaaaa aacttagtac
     1681 actataaaaa taatagagtt gcaataatgg atttaaatag ttatcttatt aaaaaatctg
     1741 ataatctatc aaagaaaata tcgattaacg aaagatctta tgaaatattt aaggatgaga
     1801 aattcataag tagtaaagta ggaaaagaag tactaaaaaa cttaaaaata gatctaataa
     1861 aggatttaaa cgtatataag acaccagagc cattcatata tttatcaata aatcgtatat
     1921 ctccacaaaa aatattaatt atagaaaata aagacactta cataacaata acaaaaatgc
     1981 tattagaagg aaaagaaata cttaaaaata aaatagatac tgttatatac ggagaaggaa
     2041 aaaaaataat caacagtatt aaaggattaa aggaagatat aacaattgaa tatcttacaa
     2101 ataaagaaaa tgaattttta tattggggag atatagataa aactggatta tcaatataca
     2161 cttccctaaa agataccaat gaaattaata atataagatt atttgaagtt gcttatgagc
     2221 atatgataga taaaacaaag gaaaatacct tgaggaatat ccccaaaaat caaaaagata
     2281 attttgaatg tggattagaa aatattaata acctaaactt aaaagaaagt attaaaaata
     2341 ttttagtaca aggaaaatac atacctcaag aagctctaaa tagattcgac ttggaggaat
     2401 ataactaatg gaaatgatta atattttaga aggctacaat aagagagtaa actgtatagg
     2461 atcttattat cttattgcaa ctcttgctaa caatagaaat aaatttaaag aatttgacaa
     2521 cattcaattt tacaatttat taattcaagt tttgtgttat atatttgata gatctcttag
     2581 aagaaagaac tgtttaagag atgatattaa ggattttatt gaagaaataa atcgtatgga
     2641 ttataaaata atgctttcag aggatgactt aaaggattta gcaaactata taattaatgg
     2701 attaaccaat agtggtaaag tatatctatt tacctactat tctttagaac aagaaaaaca
     2761 tatagatgaa agtattaaaa ttatagaaga taaaaatgta aaaataaata atcaggaaag
     2821 actatcctac tccttaacaa cagagggtta tagattgttg ttatcaacaa aagaatatga
     2881 tgaactattc caaattcaaa tatcacaaat gattgcaaag ttaaggatag aaaagggtga
     2941 ttatcaaggc gcaaaggaag atatttacga tataataaat accttagaga ttcatcaaca
     3001 gaaaattgat aattatataa aaataataaa agtaaatata ctatatataa aagaagaaaa
     3061 atattcaaca gtaatagata caactgtaga aacactttta gaagaaatgg acaaatatga
     3121 agaattaaaa aaagaagtaa aactattaat caaagataaa gaggactact tggaaaagga
     3181 tgaggatata gacaaagaaa agataaaaaa tataagaact caactattac aaatgaaaga
     3241 attaatagat ggaatagttt tagcaaagtc tacagccaca aatttgttat ctagagttca
     3301 agattttaga gaagaattta aagaaatact taaaaaacta ttaacaactc cagtaattaa
     3361 aagattcaat ttcaaggaag aaatcttaga tagacttgaa aaagacagtg attttttatt
     3421 gataataaga gaattatata tatctttatt taaagtagag ttgccaaatc gatttaatat
     3481 tggagtggca tatcaagaac agaaactatt aaatactaga gaaattgaaa atgaaatagt
     3541 taataaagac gagttagaaa ttattgaaaa taaagagaag gaggaacgta taaattattg
     3601 tgaaaattac tattttgatt tttttaaata cttaattaaa tatggacttg aaaaaggaca
     3661 atttacatta gaagaatttc taaaaaatta tctgaaatat gacaaagtta tttacttaga
     3721 aataactaaa gactccaaat tgatgagaga tataataatt tacttcgcta atcaaaggga
     3781 aagtatcaat atcaaaaaga taatcagaat gttagaaaat agtgccttct caccaaatac
     3841 agattttcaa attgaaagat taatagaacg tttattagta gaagattttg aactattaaa
     3901 tagcgttagc catattaaaa gtaataaaat atttgaagat gaaatgaaaa taaatactga
     3961 aataaattta gaaactatga ctgcagttaa gctagaaata gcaaatataa aatttgaaat
     4021 aatctaaata taaggggaaa tacaatgaat atatctttaa atgactataa aatagccaat
     4081 gaaatatttt atagattact taaaaaaagt gagttaaaag aaaaagaagc taaagaatta
     4141 tttttagctt atataaataa cagtaatgta aggtatgctt tagaggcaat ttgtgaagta
     4201 tctaaaataa aaataatatt aatttcagat accttatact taataccaaa taatgacaat
     4261 gatatttttg ggtttgacta tagaagagaa tctttattag ggaaagattt aaaagatagt
     4321 tatttaggat atttgataat gacaataata ttttctgaat ttacaaatgg ggtagcacca
     4381 gcatcatata ttcaagtatt aaacttactg gatttagtac aagaatcctt agaacgttca
     4441 atagctcgtg ataatctaga agaaatagaa aaatcatgtg agtttaatat aactatctta
     4501 aagaactatt gggaaagtaa aagaaagtgg gacgaatcta gtaagaaagg aatagaaaca
     4561 acatcagcag atttccaaat agggtttatt agaaaaataa ttaatttctt aaaaaatgaa
     4621 ggcttaattg tacacattga ggatgaagat aagataaaac caactagaag atttcttgat
     4681 cttatgaata attatttttt agatgaagaa aggaaagtat taatagaaaa actagtttta
     4741 agaaaagggg aaagtattaa tgccagtaat taataaatac aaaattataa attttaaatt
     4801 tggtatggga caagaaagaa tacttagcaa cagaaccatg gaactgtatg gagaaaactt
     4861 acaagtagaa gcagaaaata cagcaggcaa aactatgagt attcaaacta tcatacaaag
     4921 tatttgtcca ctatcggatg tagccaaacc gtttactgca atatataata aaaaagaacc
     4981 attatactca atgactgaat ggttgctaga tgatggtaaa acgttattat taacgggaat
     5041 aggatttgag aaaaaggctg gatcatctaa agaagaaggg aaagaaaaaa aggacgattt
     5101 atacaaatac tttatgttta ttatagaaga atctaaagaa atggaggttg atatagataa
     5161 tataccttta ttaaaaaaca atgacaaagg tataaggata gtacaacctt taacggcctc
     5221 acaagactat ataaaaatgc taagagataa atatggaaaa aacaaagtac acttctttaa
     5281 taattactta agaaaacaac atagagaaaa attagaagaa tatggaatta accaaagtga
     5341 atggaaagaa attattataa aacttaatag taataacaaa gaaggtggac tatcagactt
     5401 tgttaaagaa cataatactt cagaaaaact tataagagat aaaatattac ctttaataga
     5461 gacaagctta acaacagaag aattcgctcc aatattgcaa attaaggaac aagtaagaaa
     5521 gtgtataaat aatattatag acatgaatga aaaattatca gattataaaa actatagaga
     5581 tttaaacatt agagttgaaa gtttacaaaa taaaataaat aggatcctat tcatagaaga
     5641 agataaaaat aatgtattag gagaattagc taatctatat gtacatatag aaaataaacg
     5701 caatgaatta aatgaagaaa taaaacagta tcaaaaaatg aaagcatcaa aagaagagga
     5761 atttaagata gtagaatatg aagaagatag ctatgagtat tttagaaaag aaaaagagat
     5821 acaagatact caggtagaat ttgataaagc caaaaatgaa ttaaatgata aaatagaaga
     5881 actagaatta gatgaacaat atttaaatat atcaaagttc aaaagaaaaa agaaggggct
     5941 agaagaaggg gaaaaagata agatagaaaa agaaagtgaa aaacaaagaa aagaggtaaa
     6001 tcaagaagaa ataacggcta ttataaatcg tctaggtagc agtattcatt attgtttaaa
     6061 ggaagaaagt attataaata atgaaaaact taaagaaaat acaaacaaca atatagctat
     6121 acaagaaaaa aagaatcaac ttaatgatga caaaagtaag ctcacaaaag tattgataag
     6181 taaaaattca gaaattaaat atatagaagg caaagaacgt gattttaata atcaactaca
     6241 tgaatttaaa cttaataaca ctgatattaa aggctttatt tctaataatt ttttaggcga
     6301 agtaatagat tatactaggt atataaatta tttaaatata aaagtaaaat caatagaaga
     6361 aaatcttgaa tcgaaagtca tgcaaataga agaatcgaat aaacttaagg atgatataaa
     6421 tgaagctata aataatctta aattagataa tcatgaatct tatatgaaac tgcaagtatt
     6481 aaaaactaga aagaaagata tagataattt taaagaaaaa tataaaatca ttaaaacaag
     6541 atttgaaata gacaatgaat tagataactc tactaaacta tataaagagg taaaacaaga
     6601 gcttaacaaa atgaaacttc aagtacagga cttagaaaat aataaaaagc aattagaaaa
     6661 agagctagaa gatttacaaa attataaaga tataattttc gaagaagaaa tattaacaga
     6721 tctagaaaga aatggaataa aacctattga aggcatgaaa tatctattaa ccttagatgg
     6781 agatttagaa tacaaaaata atctcatatc taaaaatcca ttgctaccat attctataat
     6841 aattacaaat aaagagtatc aaaaactgaa gcaaataaac ttaaaaaata aaactaaaat
     6901 ctcaatgcct atatttgtaa gagataacat agaagatact tttataacta catctagtaa
     6961 ccttgtaatt agtaatggat taaatatact atcttctttc gataacaaag ctttagatcc
     7021 tgaaaaaaga gaaaaggata tgaatctaat caaagaacaa ataaatagta ttcgagataa
     7081 agtggagcaa tataaagcag aaattgaaat attagaagaa tttaaacttc taactaataa
     7141 aattgatttt acagctgaag acttatccat agatgtgaaa attgaaaatt ataaaaaaat
     7201 tatatttgaa ataaacaata aaattaagag taatgaaaat aatgagaaga atttatctaa
     7261 taaaattaaa gaactttacg aaaataaaga aaagctatt
//
LOCUS       DAGQWR010000175         7153 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.178, whole genome shotgun sequence.
ACCESSION   DAGQWR010000175 DAGQWR010000000
VERSION     DAGQWR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.178"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(17..1945)
                     /locus_tag="MIW95_003440"
     CDS             complement(17..1945)
                     /locus_tag="MIW95_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY3152736.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITISRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQNNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            2179..2631
                     /locus_tag="MIW95_003441"
     CDS             2179..2631
                     /locus_tag="MIW95_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY3152737.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGIATNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            2634..2942
                     /locus_tag="MIW95_003442"
     CDS             2634..2942
                     /locus_tag="MIW95_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBY3152738.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            3056..4162
                     /locus_tag="MIW95_003443"
     CDS             3056..4162
                     /locus_tag="MIW95_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBY3152739.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            4237..6915
                     /gene="mngB"
                     /locus_tag="MIW95_003444"
     CDS             4237..6915
                     /gene="mngB"
                     /locus_tag="MIW95_003444"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBY3152740.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISNIENYIDSVKNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTANEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESSKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERSIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLLFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNNSKLKVNTKTVEVNSLSHCKVQTIVIK"
BASE COUNT         2843 a          897 c         1058 g         2355 t
ORIGIN      
        1 tatgtaatat aaaaatctat actatagact tgattatgga aagaaaatta tcaaaatcat
       61 tacaattaga tatactatct ctaatatcat tgtcatgaat tatctttaaa aggatatcat
      121 aaatggagtt tatttttata taatcatctt tatttatagc taacatcatg attaatttaa
      181 cagtagaatc ttcactccaa tatattggtt cattcaatat ggaaatacaa atcttagttc
      241 ttatagagct taattccata ggatgaggta tagctaaaaa atcatctata ttggtagaag
      301 ataaactttc tctgtaaaat acagattcag caaatgatgg aaatacaatt tcactttgag
      361 ttaatttgtt gcacatcaac tttataagct catctttatc tttgatttta ggattaacaa
      421 taaataaatt tttatcaaag aaattgtcca aaagattact ataaatatgg gaattattag
      481 tgattgactt agatatattc tctatatctt tttttaatag ttttaaatcc actaatacaa
      541 taggaatact atcatgattt aaaggaattg tcgatattat tatatctaca tctgacaatt
      601 cactagctaa aaattgatta aatgatagac attgaagtat atttatttta tcatgaaaaa
      661 ctttgtttaa ttgaacctct aataacctac ttgaaccata ccctgaacca catactaata
      721 ctacgttttt acacttaata ttattttgaa aaaatctctc tattccagca cctatatgca
      781 gggatacata tccaatttca tcttctgtta aagagtaaat actattttta aaaacttttt
      841 ctatagcatt taaagttatt tcaaatgcta aaggataatt actctttatt gtatttataa
      901 ggggattcac cttatttaaa ttataagatt tagaattgag aattgattta aagtgcagta
      961 ctaaatcatc aaataataca ctatcatttc taaggtcaaa agtatattga ttgttgatta
     1021 cttctaaaat ttcttcaaca tactctataa ttttagtatc caccctattg gaaacgttat
     1081 ccaaaagaga acttttagtt ataaaatgat tatatagata aactctgtct gcctttgaaa
     1141 taattatagt atatttactc tcaatataat taaatatatt ttcaacagtt gtattcgatt
     1201 gaaaatctgt taaaacaaca tctaaaacat tatctaagca gtaaccatca agtattctac
     1261 ttatagtaat agctaaatgg ataataaagt tttttagatt aaaatctaga aaatttatat
     1321 taaatttatt aatttcactt aacactaccc tttgaagctc tattaaatct acattattaa
     1381 ataattctat ttctctatct gtaattccaa ttatatagtt ttcaaaattc ctatttatca
     1441 tattattaga aatacatctt cgaatatcta tttccttccc ttcaacttta actccataat
     1501 atggcttaga aactaatatt aaattatact ttgacaaaat aggctttatt ctttttatgt
     1561 catttagtat tgtaaattta ctcacataca agctattagc taaatcttct agttttatat
     1621 atgtattgtt atagagaata tattttaaaa tatattgatt tctttctata ggagaatctg
     1681 gtatttcact tgaatccata atatcatcag atattaatgc tagatactgt tggtataatg
     1741 ctaaattaag aatatctata taatatcctt ctcctttttt tattttaata atagcaccat
     1801 ttttctctaa agttccattt atagaagtaa tatcatttct tatagttctc tcactcactt
     1861 ttaaatgatt acttaaagtt ttacaagtaa ttttatcatc actttcattt aataatttaa
     1921 acaacgtgag caatctcttg tgcataaata tcccccctta tagaatttct tctaaattat
     1981 actatttaca ctcatataat acaattactc taataaaatt gtattataat aatttctttc
     2041 cacttctata tggaaattag ttattatcat aatataaatt tccatgtcga agtggaaatt
     2101 agttatattt aatttaatta ggataatgat tacaatataa atataagtta agacattacg
     2161 aatggagtgg aaaaatatat ggtactacaa gaaaaatata tatttataga taaagactta
     2221 aaaagcaaag aagaagtatt aaattttatt gcagatagtg cttatatact aggtattgca
     2281 actaataaag aggctctttt aaaagacctt ctaaaaagag aaaatgaatt tccaacagga
     2341 ataaaagatg gatttgcgat acctcataca aaaagtgtaa atgtaaagga agcttccata
     2401 ctctatatta aaacaactaa cgagttagag tggaaaactt tagatgatag taaggttaga
     2461 tatgtattta gtttgttagc cccacataaa aatgaaggaa atatacattt aaaaactcta
     2521 tctaaattag ccacatgttt aatggaggat gattttacaa gtgaagttaa aaatgctagt
     2581 gatgcaagtc atttagtaaa atatataact aataaaatga aggaggaata attatgaaaa
     2641 tagtagcaat aacatcatgt atagcaggat tagcacacac acctatggca gcaaaagctt
     2701 tagaacaagc tgcatcaaaa atgggtcatg aaatcaaagt agaacaacaa ggtgctatgg
     2761 gacgagtaaa tactataaca aaagaagaag cacaagatgc ggactttata cttatagcat
     2821 ctgaccaaac tatatctgga atggaaagat ttgatggtaa aaaagtgata aaagtcaaaa
     2881 taggtatagc gttaaaaaaa ccagaagctg tcttaacaaa gtgtatagaa gctatttctt
     2941 aataaaaaaa tctcactcta atatagaatg agaaaaataa aaaattaaaa aattgtctaa
     3001 tgtaattata acatattatc aaaaaatata acaaacttat ataggaggat taaaaatgag
     3061 taaaaaaaat aaatctcttt taagtgaact taagggacac ttaatgacag gtatctcata
     3121 tatgttgcca cttgtaatag gctcatcttt agttgtagct atcccaaagc ttacagcttt
     3181 agcaatgggt ataacaagtt tagatgctta taaaaatgta tctggttttt atcatattct
     3241 ttacttaatg gaacaagttg gttggactgg cataggtcta ttaaatacgg tattagcagg
     3301 atttatagca ttttcaatag gtgagaaacc agctatgggt gcaggtttta ttggtgggct
     3361 catagcaagt aatactaatg caggattctt aggagcagta atagcaggtt tctttgctgg
     3421 gtatgtttgt aagtttttaa agaaaaaaat taaaatatct gggtcagcat caggaatgat
     3481 gccacttata ataatgccac ttataacagt tggattaact ggttttttaa tgagtgttgt
     3541 attagcagga cctttaggaa acataaatac ttcattaaat gcttgggtag ctgaaatgtg
     3601 tcaaaatgga acaaattcgg tggtattggc tttaatttta ggcgcaatga taggattcga
     3661 tttaggtgga ccagtaaata aagcagcttg gatggcagga aatgcattat tgttagaggg
     3721 aatctatcta ccagcgatat tggtaaactg cgcaatagtt atacctccac taggatatgg
     3781 tttagctaca ttcataagaa aatcaaaatt ttccaatgca ttagctgaaa caggaaaagg
     3841 caatatagtt atgggtataa ttggaataac agaaggtgca attcctttca ctttaacaaa
     3901 tccattaaaa ttagttcctg ttaatatgat aggatgcgct ttgggtgtag gattatcagc
     3961 tttgttaggt gtacatgcta taatgcctcc agttggtgga ttatatggat ttatatcaat
     4021 aggttctgga tgggcttact tagtaggtgc aatatttgga gctttagtaa tagcaatatt
     4081 atcaacaact ttagtagact tcaatgaaga tgaaactacg gaaaatacag aatttgatga
     4141 aatagagtta gatataatgt aaattaaaaa cttaatttta gataattgag aattgagtgt
     4201 actctcaatt ctcaattaaa ttttgggagg atttttatga aaaagacaaa agtacatgta
     4261 gttccacaca gtcattggga tagagaatgg tattttacaa catcaaggtc aaaaatatat
     4321 ttaatgaaag attttcaaga tatattagac atattggaag aaaaagagga ctttaaatac
     4381 tttactatgg atgcacaagc ttctctctta gatgattatt taaaatggag acctcaggat
     4441 gaatatagaa taagagattt agttcaaaga agaagattag ttattggacc ttggtatact
     4501 caaacagacc aattggttat atcaggagaa tctataatta gaaaccttta ctatggaata
     4561 gatagatgca atgaatttgg tgaacctatg agggttggat atgtaccaga ttcttttggg
     4621 caatcagctc aaatgccaca aatatacaga ggttttggaa tagatagttc attattttgg
     4681 cgtggagttt ctgatgatat ggtagataca acagagttta tatggaaagg ttctgatggt
     4741 tcaaaagtat tagcagttca aataccattt ggttattact atggaggtaa tataccagaa
     4801 gatgattcag atttaaaaga atatttaaaa gatataattg gaaaacttaa ggttaaagct
     4861 tctactaata acgtatattt tccaaatgga tttgaccaag caccaataag aaaaaacctt
     4921 cctgatatat taaaaaaagc taatgaaata gataatgaaa atgaatacga aataagtaat
     4981 atagaaaact atatagattc agtaaaaaat gaaagaaatg aatttacaga gcttaaaggt
     5041 gaatttttaa atgctaaaca tatgagaata cacaaatcca tattctcctc aaggtcagac
     5101 cttaaaataa tgaataacaa attagaaaat tatatcacaa atgtacttga accattatta
     5161 acattaagtt attcacttgg aaatgaatac cctcatctag ttataaaaga tatatggaaa
     5221 cttatgtttg aaaatgcagc tcatgacagc ataggttctt gtaatagtga cacagcaaat
     5281 gaggatgtat acacaagata caaacaagcc agagatttga gtataaattt attagatttg
     5341 catatgagac ttataagtac tcaaatcaaa aataataatg aagaaataac attaacagta
     5401 ttcaatggat tcccagaagt cagaaatgaa gttgtagaat ttgacactta tattccaggt
     5461 caagattttg tgataaagaa taaaaatgga gaaattctta attatataat aaaagaaaaa
     5521 gaagatatta caaattatat attaactcaa acaataagat taaacccaag taaaaaaata
     5581 tatataccca aaactgtata tagagcaaaa attgcacttt cagtaaaaga tataccacca
     5641 atgggatata ctcaactagt ttttgaattg gatactaaaa ataaatgtga aatagaaaca
     5701 agtaattgca aagaaataga aaacaaatat tacaaaataa cagccaataa aaatggttca
     5761 ttagaaatat tagataaaga atctagtaag acttataaaa accaagctgt aattgaagac
     5821 aatggagatg atggagattc atacaactat tctcctccaa gaaaagatat ttatgtttca
     5881 tctttagatt ctgtatcttc agtagaagtg ctaaaatcaa atattcaaga agaaatgata
     5941 cttaaatatt cattgaccat accagcttca ctggaagaaa ggtctatagg taaagtttct
     6001 gtgaaaatgc cagtaacaat gaaaattacc ttagaaccta aagaacaaat aattaagttt
     6061 aatgttaata taaaaaataa ccaagcacta tctcatagag taagagtttt atttaataca
     6121 gaaatagcat ctaagttttc tatagcagat caacaatttg gaataataca aagaccaaca
     6181 gtattagaaa aagaccttgc attatggaaa agagacaatt actcatggca agaaaaacct
     6241 ataactatcg aaccaatgca aagttttgtt actttagagg atggacaaag aggaatagca
     6301 ttaataactg gttgtgtaag agaatatcaa atagttggtg ataatttaga tacaatagca
     6361 ttaacactat tcaggtcatt tggatatatg ggaagagaaa atttacttta tagaccagga
     6421 agagcttctg gagaaaaaat aatagcaaca ccagatgcac aattacttaa agagttaaac
     6481 tttgactttg gtctttacat atataacaat aaatttgatg aagcaaatgt agctaataca
     6541 gctaagagat tcttaacacc tatacaaata tatgaatatg ctgatttttt aaatggtaga
     6601 cttctatttg cattcaatga cgaaaaacaa atatatgaaa atgaatttag tttgatgaat
     6661 gtaaataatt caaatttcac agtaagtgca ataaagaaag aagaaaaagg agatggaatt
     6721 gtagttagaa tcttcaatgg tatgaaaaat gaggatgcta agggaagtct taacatcaat
     6781 aaaaatgtca atgatgctta tagtgcaatg ctcgacgaaa tctatgataa taatagcaag
     6841 cttaaagtaa atactaaaac tgtagaggta aattctttat cacattgtaa agtacaaaca
     6901 atagtaatta aataatataa aatagcttat acaagatttc aaaataaatt gctagtacta
     6961 tgttaatagt aaacccagat attatgctat ttataagctc tgtaacacta aataacattt
     7021 tgtaaaataa aaaggtgaac cttgtatatt aggattcacc ttttttaaat aaatcaacat
     7081 tctgttttaa aataacattt ataagctgtt ttgaaataat acttacaaat tattttaaaa
     7141 caatacctat aaa
//
LOCUS       DAGQWR010000176         6995 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.179, whole genome shotgun sequence.
ACCESSION   DAGQWR010000176 DAGQWR010000000
VERSION     DAGQWR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.179"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(134..973)
                     /locus_tag="MIW95_003445"
     CDS             complement(134..973)
                     /locus_tag="MIW95_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBY3152741.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(997..1755)
                     /locus_tag="MIW95_003446"
     CDS             complement(997..1755)
                     /locus_tag="MIW95_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY3152742.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNENVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1802..2275)
                     /locus_tag="MIW95_003447"
     CDS             complement(1802..2275)
                     /locus_tag="MIW95_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY3152743.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDEHLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2304..2720)
                     /locus_tag="MIW95_003448"
     CDS             complement(2304..2720)
                     /locus_tag="MIW95_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY3152744.1"
                     /translation="MIHMTGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRRLEDRIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2767..3819)
                     /locus_tag="MIW95_003449"
     CDS             complement(2767..3819)
                     /locus_tag="MIW95_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBY3152745.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGTIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPMTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4215..4895
                     /locus_tag="MIW95_003450"
     CDS             4215..4895
                     /locus_tag="MIW95_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBY3152746.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5028..5348)
                     /gene="sugE"
                     /locus_tag="MIW95_003451"
     CDS             complement(5028..5348)
                     /gene="sugE"
                     /locus_tag="MIW95_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBY3152747.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5730..6557
                     /locus_tag="MIW95_003452"
     CDS             5730..6557
                     /locus_tag="MIW95_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBY3152748.1"
                     /translation="MNFYKIAVCQMMTTENKIENINHAVDMVTEAAINGAKIIVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEYGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGVEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2358 a         1280 c          829 g         2528 t
ORIGIN      
        1 tatataagtt taaaatttaa tctattataa aattacccta attgctaaac tttttaatat
       61 aaattttcca acatcttaca aatataatca atagatatag aatcctaatt tacacaccaa
      121 catatatatt tatttaaaat agtcccaaaa tacctgtaat agctcccact actatcagta
      181 taaacattat ttttattgga gaaaggcctt tttttaaagc aaagagtact aataaagtaa
      241 gtaccaaagg aattaatcct ggaaatatct tatcaagtaa atccgtttga agactaaatt
      301 taaaatcacc actagtataa cttattggtg tagataaatt aataaaccta cctacaagtc
      361 cccccatgac taaagtacct aatatagaag ccccttctat aaccttattt actattccac
      421 cttgaagtat actttcaaca gcttttttcc catacttata tccatttgtc cacattccaa
      481 aagaaattga agtcattatt atatattgag ctattacaaa taatatagga cctgcaattg
      541 atgctcctcc ctcagttaaa ccaatacaaa cagctaacac tataggagtt ataactcctt
      601 gagttaaagt atcaccaatt cctgctagtg gacccatcaa gcctgttttg atagcattca
      661 taccatctcc acttatttca tctgctccat tagctttttc ttcttccata gctattgtta
      721 ttccatgtat aacacatcca catataggtt ctgtgttaaa aaatgctaaa tgagtctcca
      781 cttcttgttt taactcttcc ttatctggat ataatttttt aagtactggc aacattgaat
      841 gtgagaaagc tgttgattgc aatctttcat aattgtaatt agattgcgca aagaaaaacc
      901 atcttaacca tgattttatt acatcttttt tgcttaacgt tttcttagat acttcatttt
      961 ttatatctga cataatattt tccccctcac atgtatttaa gatgtagcac tatcttttcc
     1021 gtacttaatt gaagtaattg taagagcaag tattactcca ataacagata ttactattat
     1081 atctacttta aaataaacag ccattagtat tcctaatatg aaaaagggca tagtaaactt
     1141 gtttccaatc aattttaaat tcatagcaat cccaagtgct ggcaacatag ccccaattac
     1201 attaaataca tgtaacacat tctcattcat tacttccaac ataccatcta tcgcatttgg
     1261 accaaaataa acaactaata atgctggtat aaaactcatt gcaaataaca gtatctgtgg
     1321 tggtactata tttataaatg ctactctatc aacattccct ttttgaacct ctctatcagc
     1381 ccaatgtgca aaaaaagaat ttactgtcat ttttccaatc caaagtacag ttgcaacagt
     1441 tcccaatggt acagctattg ccattgcagt acttacatct agcttagatg ctatagctaa
     1501 agcagtacct acatatcctg caaaagctgg gtcccccatc tgagctcccc ctgctgatat
     1561 aaaacctaga tatatcatat ttatacctgc tcctataatt gctccttgaa cagggtctcc
     1621 taatataatt cccgttacaa atccagccaa tagaggtcta ttccatgtaa aaaatccaac
     1681 ataggtcaac cacggactcc aagaaagata ataaaaaatt gctattaata ctgcttgaat
     1741 tagtgaaatg tgcatattct tcctccctat tttcttttaa agatttacaa gatagtcttt
     1801 tttataataa tgttcctaca tcaattttct ttgcatctgg tactatttgt ataaagcatt
     1861 taacatcttt tgatattagt tctctaaata tttctttttc ttcatctgac acagatatat
     1921 ttttataaaa actttttcta ccaagccctg ctcccatacc tccaacattc aattccttta
     1981 aatctacccc actatttact aaacctaaga cagttttagg gtatttgacc aatactatta
     2041 aatgttcatc tccattatgt gattttaaaa cttcagcacc tgattctaca tcatgtactt
     2101 caaccttaac agttgatgga gctgccattt gtaaaacttg aactataaat gggtccttta
     2161 ctactaaatc atccacgatt actatccttt ttgccgatgt aatcttacac caagcagtaa
     2221 taacttgccc atgtattaat ctatcatcaa ttcttgttaa tgctatatta ctcataatta
     2281 ttcctccaga tttttaattt attttacaac tttaaaacag ttttaacatc tttgattcct
     2341 gatatcccaa cttcctcaca taaattcttc aagtctttta acttcatagt atctctgttt
     2401 aaaaatagct ctaaaagcat aggtaaattt acacctgtca aacattctaa ctcacatacc
     2461 ttagcaaatt tatttattat aatagcagat gtattaaatg gagtcccgcc ataaaaatca
     2521 agcaatacta aaaccccttc atccttatat ttctcaattc tatcttctaa ccttctacta
     2581 aactcatcta tgctgtcacc atggtttaat cctaaagttt ctacattttc ctgtttgcct
     2641 actattaatt cagcactttc taacattgct ttagccatat ttccatgtgt ggctataatt
     2701 atccctgtca tatgaatcat acctttctta accttgttta tatggattat tatttagtac
     2761 tctaatctaa ttatctatat taatcattat cttaccaaag aaaatatccc ttttgtatac
     2821 tttttcaaaa taagctggta cttcttctaa atttattcta tgtgttacta atttttcaac
     2881 attcatatca cccttttgca tatgatacaa tccagcactc cattcttttc ctgggaatgg
     2941 aaaagagttt ccaaaccaag ttcccttgac agttaattca cttcttacta tcttttcaaa
     3001 ttgttctcta gtcaaagcta catccccata tggtacacct gcatacaata ctgtccctcc
     3061 ctttttagct aacaacaata cttgcccaca tgtcattgga gtcccagccg attcaataac
     3121 aatatctgca ccatctccat cagttaacct ttttatttct tctacaatat tcttttcttt
     3181 tgcatttata caaatatctg cccctaactc tttcgccaag tctaacttct catcaaatac
     3241 atctacagct attatttttg tagagccaaa tatctttgcc cattgtattg aaaataatcc
     3301 tattgtacct gtaccaagta ctacaacagt atctccaact ttagcttctg atctaaaaag
     3361 accatgccct gctatacata ctggttctag cgcagcggca gtctcataac ttatctcatc
     3421 tggtattttt attaaattcc tctctttcac ctttgtatac tctgcaaaac aacctccaag
     3481 ttctttattt cctattattg ctacattatt acatcttgaa tacaatcctt ttttacattc
     3541 atcacattca aaacatggca tagctggaca aacagcaact ctatctccta ttttaaaact
     3601 ccttacttct ttaccaacct gtgccacttc tccagaaaat tcatgcccta aaatttctcc
     3661 aaccatatgt ggacctgtct ttgaatattt tgatatatca gaaccacata ttccagctac
     3721 ttttacttta attattacat catctttttc taatattttt ggtacatcca catcctccac
     3781 tcttgtgtct cttataccat agaatctaac tgacttcact attaaccacc cctacttttt
     3841 atttgagaat atatccctat tgtttatatg ttaaatatct aaagcaactt tcatgccaat
     3901 atatgtaagc attgcattca tgcattttat aaaattatca ttaaatgtgt gtcaaagtgt
     3961 gtcaaaagtt tcttatatta aaacttatgt gtgtcaatat aaacgacact aacattttat
     4021 attagttctc ttattataag ttttataaaa tgtacttttt ttgttatttt tataaaattg
     4081 ttcggaaatt tcccttctat ttgtgatata atattaaata taaggtatca atataaattt
     4141 tcatgcgaat atcagaactt ttttgacact aatgccttat tcaatataat aatttaactt
     4201 tttggaggtt gtttatgaaa aaagtgtatc aaggaaagac taaagatgtt tttgaattag
     4261 ataatggaaa ttacttactt aaatttaaag acgatgtaac tggtaaagat ggagtatttg
     4321 acccaggcga aaatagtgta ggtctaagta tagatggaat tggtagagct aatttagaga
     4381 catctgttaa gttctttgaa attctaaaca atgctggaat taaaactcat tatgtaagtg
     4441 ctaatttaga agaagcaact atggaagttc taccagcaaa agtttttggt aatggattag
     4501 aagttatttg tcgttataga gctgttggta gtttcttacg tcgttatggt tcatgtgttg
     4561 aagaaggtgc taaactagat tgctatgtag aagcaacttt aaaagatgat gaccgttgtg
     4621 accctcttat aacatctgaa ggtttagaag ctttaaacat tatgactcaa gctcaatttg
     4681 attcaatgaa aagcatgact caaaaaattt ctaatatagt tcgtgatact atagctgaaa
     4741 aaggtcttga actttatgat atcaaatttg aatttggatt ctacaatgat gaagtaatct
     4801 taatagatga aatagcatct ggaaatatgc gtgtttataa agatggtgta atagttgacc
     4861 ctatggattt aactgctctt ttattagata aatagtatat acatataatt ttcaaatata
     4921 ataaacttat aaataagata agctatgaag taaagacttt ttaaatgttg attattttta
     4981 aacattttct cgcctttaat ttcatagctt atttaaatta atatactcta attagtagat
     5041 aatagcttta accctacaat tcccaccaca ataaagccta tacatgcaat tcttgatata
     5101 ctaataggct catttaagat agccatgcct acaatagctg taccgacggt accaattcca
     5161 gtccatattg catatgaagt tccaagtgga agatgtttta gtgctaatga caaaaaataa
     5221 aaacttacaa tcattccaac aacagtaagt atacttggag ttaatttaga aaatccatgt
     5281 gaatttttta gttgaaaagc ccaaaacact tcaaacaaac ctgcaacaaa caaaaataac
     5341 catttcataa actcatccct cttttcaaat gtatctctta tttttccaat aaaataacct
     5401 gagaaatatt ataaaatatc tcccaggttt ttgtccttcc gtgttcacaa caaaaactat
     5461 gttgtgggtt ttatctcgga ccaggccaat cattaataat tgcaaaaccc tataaaactc
     5521 tattaattat tctattttat ctataataat actatcaaca tatataaaaa aagtcaatat
     5581 atgggttcca tctataataa aaataacttt tatatacctt ttaatattct tattcacttc
     5641 tctccatacc catttaggtc aatctaagaa tatctaattt ttatttaata tgttataata
     5701 acataaaaat attgaaagga gtaatttcta tgaatttcta taaaattgca gtatgtcaga
     5761 tgatgaccac agaaaataaa attgaaaata tcaatcatgc tgtagatatg gtaacagaag
     5821 ctgccatcaa tggagctaaa attatagttt tgccagaaat gtttaactgc ccatatgaaa
     5881 ataaatattt tcctaaattt gctgaagaat atccaggtga aacaacaacg atattgagta
     5941 aattagctga gaaacatgga atttatctag tttctggtag tattcctgag ttagaatatg
     6001 gaaaaatcta taatacttgc tatgtatttg ataaaaatgg agctctaatt ggaaaacatc
     6061 gtaaaatgca cttatttgac attgaagtta caggaaaagt tagttttaaa gaatcagata
     6121 ctcttacagc tggaaatgat gtcactgtaa tagataccga atatggaaaa gttggtatag
     6181 ctatttgcta tgatattcgt ttccctgagt tatcacgttt aatggctctt aaaggtgttg
     6241 aaatcgttat cttacctgct gcatttaata tgactactgg tcccgcccat tgggagttat
     6301 ctataagaat gcgtgcttta gataatcaga tattctatgt tggtgctgca cctgctagaa
     6361 atatgaatgc ttcttatata gcttttggaa attcaagaat ttcagaccct tggggaagaa
     6421 taattgctca agctgatgaa aaagaatgta ttatttatgc tgacattgat agagatttaa
     6481 ttcctgacat aagacaacag ttaccacttc ttaagcacag aagaactgat ttatatgaac
     6541 ttaatacttt gaaataaaac agaacataac cccataaatc ataaattaag tgttaaaaaa
     6601 atttatgggg ttctcattaa tttgatttaa aattaattta ataaaaataa tgctaaaatt
     6661 aatttaatta aataatacta ataattacta atgattatgc taattgtcag catttactaa
     6721 tttaatcact attagttatt tcttttatta aatatcttta ctttttttac ttattatgtc
     6781 ttctagcttt acatgtggat tatggtattt ctttctattt ttagtcacat ctttctattg
     6841 aattgcttgc ttaaaacacc aatgcacaca tgccagataa tgttcacaat tatgtaaaaa
     6901 tattgctcta ccttcttcaa tgttgatatt atctctccta gttaatatta tctttcctaa
     6961 aatttctttt aattaaaaaa caacattagt tgata
//
LOCUS       DAGQWR010000177         6971 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.180, whole genome shotgun sequence.
ACCESSION   DAGQWR010000177 DAGQWR010000000
VERSION     DAGQWR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.180"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..198
                     /locus_tag="MIW95_003453"
     CDS             <1..198
                     /locus_tag="MIW95_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152749.1"
                     /translation="ITNDENNILTDLINESIVNYILENKKHNIENKDLEEVIKFYKKE
                     NTEKPKSAARAIAEAKTHTLY"
     gene            complement(627..1004)
                     /locus_tag="MIW95_003454"
     CDS             complement(627..1004)
                     /locus_tag="MIW95_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY3152750.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            1345..2457
                     /locus_tag="MIW95_003455"
     CDS             1345..2457
                     /locus_tag="MIW95_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBY3152751.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(2665..3141)
                     /locus_tag="MIW95_003456"
     CDS             complement(2665..3141)
                     /locus_tag="MIW95_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152752.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQPNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(3340..4965)
                     /locus_tag="MIW95_003457"
     CDS             complement(3340..4965)
                     /locus_tag="MIW95_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY3152753.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            complement(5303..6322)
                     /locus_tag="MIW95_003458"
     CDS             complement(5303..6322)
                     /locus_tag="MIW95_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBY3152754.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(6319..6888)
                     /gene="mocA"
                     /locus_tag="MIW95_003459"
     CDS             complement(6319..6888)
                     /gene="mocA"
                     /locus_tag="MIW95_003459"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBY3152755.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
BASE COUNT         2462 a         1138 c          688 g         2683 t
ORIGIN      
        1 ataactaatg acgaaaataa tattttgaca gatttgataa atgaatcaat tgtaaattat
       61 attcttgaaa acaaaaaaca taacattgaa aataaagatt tagaagaagt gattaaattt
      121 tacaaaaaag aaaatactga aaaacctaaa agtgcagcaa gagctatagc tgaagcaaaa
      181 actcataccc tgtattaatc aaataaaaaa gttcctaaaa actaggaact tttttatatt
      241 aataattatt aatatatctc aatatttatc tcaaatttat caatataagc atgtttaata
      301 caatctattg tatacttatc ttaccctaga aataatcaaa acaaaaatta tgccctttat
      361 atctaacaat taatttacat gattaatata caaaaattct gacttttaaa ttcgtataca
      421 atatcaaact aaaataatac taatgacaaa cccaatataa attaaattca aacaggtttg
      481 attttacatc aaacttattg tattaatatt aaataacaag acaaaatatt tataaaaatt
      541 acaaatttta cataaaactt tatattttat tcatttaact aattattgta aaaactattt
      601 attttatgat aattaactac accaaattat tcatcttttt taatatcttt aacccatttt
      661 tcaacagagt tcaattcttc ttcatttacc ccctttttga acaaggattt cataaaactc
      721 tcaatagaac ttccatgcat attatctaaa aaagtttttg tttcaaaatg taaatactct
      781 tcttctccaa taagaactgt ataatgtgta tgtctatcaa tctttcttgc acttaagaat
      841 tgcttattta ctaatcttga taaaagtgtt agtgttgtag tttgcttcca attatatagt
      901 ttttccatag cttcaatgac atcttttgat gctactgtaa aatctgaact ccaaataaat
      961 ttcatgactt ttaattctgc tggtggtatc ttagtaatag tcatatgatt ttacactcct
     1021 ctcaaacttc tacataatta aatactatat taatagactt ttcgagtcaa aggttttatt
     1081 taaaatatct catccaaatt tattaataca aaaaatttat atttttattc aaaattaaac
     1141 acacaatcta ttaatataaa gataaatatc tattctataa gtataacaga ttatcaaatt
     1201 aaaattctaa tatttgacat aatatataca ttttttaaat aaatatatac aatgtattaa
     1261 aatgtatgca actctatttc aaaattattt tgtgatatat atatgttata ataaatgtat
     1321 aaaattttga ttggtgggtt atatatgaat gcatttatta gaaaaagaaa taaaaactat
     1381 gttgtttacc tggaatttga agatgaaaag acaggtaaaa gaaaacaaaa aaatatgggt
     1441 acttttgata aaaaaaggga agcaagtaaa agactaaatg aggtaaaaga aagcttatat
     1501 aatgatggtt ttttagtacc aagcgaaatt acagtgtcag aatttctatt agattttcta
     1561 aataaatata gtgaaaatat atctttagct acatataata attatgttag tatttgtaaa
     1621 aattacatta atccatctat tggaaaatat aaaatacaag acttacatcc ggttcacata
     1681 caaaactaca tagataaact atcttataaa ctaaatcctc aatcaattaa gattcatata
     1741 aatatattga aacttgcaat aaaaagagct tatagactca aattaataaa agaaaatgtt
     1801 atggatagta tagaggctcc aagatataaa aaatttaaaa atgagattta tgatagagaa
     1861 caaatgataa aattattaga tttagcaaaa aatacagaaa tggaattacc tattaatttg
     1921 gctattggat taggtcttag aatttcagaa gttttaggtc taacttggga taatattgat
     1981 tttgaagaaa atacaataac agtaaataag ataacttccc gaattaatgg ttctgttatt
     2041 ctcaaagagc caaagacaga aagctctgtt agaaaaatat cttctcccaa agaattaatg
     2101 tctttattaa aagaatataa aataaaacag aataaaaatt tattaaaatc ttccattaga
     2161 aataataata atttgttgtt tttcaataag aaatgtgaac ctattgctga agatgttatg
     2221 agtaaaaaat ttaaaagatt tttagaaaag aatgaattac ctcatattag attccatgat
     2281 ttaagacact cacatgttac tttacttata aattctaaag tacctataaa ggttatatcc
     2341 gagagagtag gacactcaaa tataagtact actctaagcg tttattctca tgtacttaaa
     2401 gaaatggata aagaggcttc tgacaaaatt tctgaaagtt tatttaatgc taattaatat
     2461 taatttatct tagctttaaa ttagcttagc ttattgagat agattcatta atattaaatg
     2521 tatctatctc aaattaataa ctacttaaat aatctgtttt attttaattt atcgcctcag
     2581 ttattatact tttttatgaa aaattatact ttacaataaa aaagatattt ccttatagaa
     2641 agtatctttt taaatactat tttattatat acttgctaac attggaatat ctaacatctt
     2701 cttcattaca tcatctactt tttttgcttt atattcattg tatccaatta aatatccttt
     2761 ttcatatgaa ctttttaaaa tctctttttc atataaatta tcagatttgg cttctgtgac
     2821 acttaaatta taaagccctt tacacccatc ttcataacct ttattatacc ctttttctgc
     2881 aaaatagttt ttatgatttt tttcgttatg tactgaagat attgttactc cagaattttc
     2941 ttttgtacta gcataaggac aaaaaccttt atttggttga tgtgctgaat gtcctccaca
     3001 ataataacgt gttaactctg atttattttt attatgatga cctcctgatg aattatgccc
     3061 tcctgagtga gcatatgaaa aattccctac actaatcata gctaaactca atactacagc
     3121 tactaacctt ttcttaacca taatctattc cccccaaaat gttatatatt tttattatat
     3181 catctactat attatattta aattcttaat atacaaaata ttactcgatt ctgctaataa
     3241 acatatttaa acaacataaa aaacctccta atatttgatt aaaaatttac acactcatgc
     3301 atagcatgta tcaaatatta aaaggtttta tactaataat tatttatctt ttccatacat
     3361 tttagctaat tggtcttgta tctctttact ttctaaatac tcatcaaagt ccatttttct
     3421 atccattatt ccatttggag ttatctctat aattctattt gctatagtag atataaattc
     3481 atggtcatga gaagtaaata aaagcactcc tgggaacttt tcaagtcctt tatttacaga
     3541 agttatactt tctaggtcta agtggttagt agggtcatct aatacaagta catttgcatt
     3601 tgaaagcatt agcttactaa gcatacatct aaccttttct cctccagata taacttgagc
     3661 ttcttttaat gcttcttcac ctgagaataa cattctacct aaaaatcctc ttataaaact
     3721 ttcacttttt tcttcagaga actgtctaag ccagtcaacc aaagaatatt ctactccgtc
     3781 aaaaaactta ttatgatttt ttggaagata atcttgagaa gtagttactc cccatttata
     3841 ctctccactg tctggttcaa gttctcccat aactatgttg aaaagtgcag tagtagctat
     3901 ttcatctccc ataaatacta ctttatcatc tctatccaat ctaaatgaca cattatcaag
     3961 aaccttaacc ccatctatag ttttagttaa attgtgtact tctaaaactt cattcccaat
     4021 ttctcttgca ggtgtaaatc ctacatatgg atatcttctt cttgatggtt gtatatcttc
     4081 aacttctaat ttctctaact gtttctttct tgatgttgct tgtttagcct ttgaagcatt
     4141 agaactaaat ctagctataa attcctttaa ttgagctatc ttttcttcag tctttttatt
     4201 ttggtcctta gccagttgta aagccaattg acttgactca taccagaaat catagtttcc
     4261 aacgtacatt tgtattttac caaaatctac gtcaactata ttagtacata tttgatttag
     4321 gaaatgtcta tcatgtgata ctattataac tatagactct tctaaatcca ttataaaatt
     4381 atttaaccag tttattgatt taaaatctaa gtggtttgta ggttcgtcca ttaatagtat
     4441 ttcaggctta ccaaaaagtg attgtgctaa caatacctta accttttccc caccaactag
     4501 ttccttcatt tgtttgtaat gcatatcttt cgttatgcca agacccataa gtattttctc
     4561 agcattagtt tctgcatccc atccgtcaag ctctgcaaac tcaccttcaa gttcggcagc
     4621 ttttatccca tcttcttcat taaagtcttc cttcatatac aatgcatctt tttctttcat
     4681 tatgttccaa agtctctcat gacctcttat aacaacattt aaaacagttt cttcttcata
     4741 ttcaaagtgg tcctgtttta aaactgacat tctttcttta tcagttatag atatactacc
     4801 tgtattaggc tctatttctc ctgataatat ttttaaaaaa gtagattttc ccgcaccatt
     4861 agctcctatt attccatagc aattaccttt agtgaatttt aagttaacat ctttaaatag
     4921 ttccttatca ccaaatctaa gtccaacacc tgtaacttgt aacattaata tattttcctt
     4981 tctttttgct cataatttat tttatcataa aactttgata acatttttat tatatattat
     5041 cacgtaaata gtatattttt tatactaaat ttgtgaaata tttattaaaa taatactaaa
     5101 aattgtaaat ttataaaaat taacaatctc ttatattata cttataaaga aatcaaattg
     5161 caactttatt cattctttta cacattgaaa ataagttatt ataatttcta aataaatatg
     5221 cttttcttaa aacatcattc tagctgtttt gtttcatatt taaaaagagt acttaatttg
     5281 taaatactct tttttaataa ctttatattt tagcaaaatt acatgcagga taagtacata
     5341 catcacagtt catacaaagg ccaccatgac catatctagc tatatcatac ttatcaagct
     5401 tttcatcaat taaaacccta gataaaacta tatcaaatac tgtagtttta tgatacattg
     5461 cgcatcctgg tatacccata attggaactt ctccataata agcaagtaaa aacatagctc
     5521 ctggtaatat tggtgaacca tatgtcacta aatcagctcc agtttctttt atagctgttg
     5581 gcgtaacatc atctgggtct acactcatcc cgccagtaca acagataagt tctgctcctt
     5641 gacttataaa ttcttttatt gcactcttta taatttcttt atcatctggg catatagtct
     5701 gtcctataac ttcacaacca aagcctttta ctttttcttc tattactggt ccaaatttat
     5761 caacaattct gctataaaac acttcatttc ctgtagtaac aattcctact ttcttaggtt
     5821 taaaaggtac tacatttact atttttctat taccaactac tttttttgca tcttctaatt
     5881 ttttctcatc tataaccaaa ggtatcactc ttgttccagc tacttttaaa cctttattta
     5941 ctataaaatt attatgaaga gtagccatca ttatttctcc aacacaattc aaatcaaaaa
     6001 gtgcatcaac atctatcttt aatagaccat cacatgctgc tataaaatct atttttcctt
     6061 cttttatttc actaaaatct aaattttctc cagcagttag ttcttttaaa aacaatgcgc
     6121 cttcattttc atgaaccatt ccttctgact tttcccatac atacaaatta tctttaccaa
     6181 gagataataa cactggaata tcttcttctt ttactatgtg acccttttta aattttcttc
     6241 ctttaaattc tccaggtatt atttgagtta tgtcgtgaca taaaacttgt ccaacagcat
     6301 ctatagtttt tattagcttc ataatttacc cccgattctc tcatagtcca tttttgtatc
     6361 gatatcaaat aacatttctt taggaacttc tacatacttt atttttgatg aattttttaa
     6421 aatggtttta cctttttcat cttctttaag tgccaacaat tcttctttct tactatatgg
     6481 gaatatgact ggatttccac acatatcttt gtatcttgga attactataa aatctctatc
     6541 taatttaaaa gtatttataa ttttatctat atataagtta tcaataaatg gctggtctcc
     6601 tacaaagaac atataaccat cacattttga gctatttaaa actcctaatt ttatagattc
     6661 actttgacct atatttgcat ttttattatc tatatattca aaaccatatt tattacacaa
     6721 taacaaaacc tcttcatatt gacttactac tacaacttga tgaaaatcaa tcttgctaac
     6781 ttctctaaat acatgttcta ttatagaaca gcctttataa tttaataaaa gtttatttgc
     6841 tcccattctt ctggcaaagc ctgatgccat tatcactgca tttatcatat atatcatcct
     6901 tggtatttat aatatattga tagttctatg atttttataa tatctctatt gatattatct
     6961 atatatataa t
//
LOCUS       DAGQWR010000178         6894 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.181, whole genome shotgun sequence.
ACCESSION   DAGQWR010000178 DAGQWR010000000
VERSION     DAGQWR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.181"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(28..189)
                     /locus_tag="MIW95_003460"
     CDS             complement(28..189)
                     /locus_tag="MIW95_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152756.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(530..970)
                     /locus_tag="MIW95_003461"
     CDS             complement(530..970)
                     /locus_tag="MIW95_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBY3152757.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(1042..1869)
                     /locus_tag="MIW95_003462"
     CDS             complement(1042..1869)
                     /locus_tag="MIW95_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152758.1"
                     /translation="MEFESGIKLGKLTLIKYLGRNKHSKKVWLCQCECGNKVERVENN
                     LKQSIKNEKPPHCGCSPNWKGQNKRFKDITGKTFGKLTVLKMSGKDKYSHNLWLCQCE
                     CGNRTITSTSALEQGKAQSCGCIRKEILLERSTTHNKSNDSLYRVYHRMIKRCTNKSN
                     KDYNYYGGRGIEVCNEWLEDFINFYNWSIENGYRKGLTIDRINVNGNYEPSNCRWVTW
                     EVQRNNKRNSIRVNYKGEMITLKQCAENLNVKYLFLYNQLVTKKIPLEEVIKNIGME"
     gene            complement(1928..2398)
                     /locus_tag="MIW95_003463"
     CDS             complement(1928..2398)
                     /locus_tag="MIW95_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBY3152759.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVESFALLNGML"
     gene            complement(2415..3725)
                     /locus_tag="MIW95_003464"
     CDS             complement(2415..3725)
                     /locus_tag="MIW95_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBY3152760.1"
                     /translation="MALGGGTFVTQNKILPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEAFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVILLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSIENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAIKVINAMSKLYMTVSVN"
     gene            complement(3726..3902)
                     /locus_tag="MIW95_003465"
     CDS             complement(3726..3902)
                     /locus_tag="MIW95_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152761.1"
                     /translation="MSKALSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            complement(3895..4332)
                     /locus_tag="MIW95_003466"
     CDS             complement(3895..4332)
                     /locus_tag="MIW95_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152762.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            complement(4681..5487)
                     /locus_tag="MIW95_003467"
     CDS             complement(4681..5487)
                     /locus_tag="MIW95_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152763.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNKLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            complement(5597..5887)
                     /locus_tag="MIW95_003468"
     CDS             complement(5597..5887)
                     /locus_tag="MIW95_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBY3152764.1"
                     /translation="MARWGSVDFREFKRVCKKMEKFTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLREGWSGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            complement(5887..6234)
                     /locus_tag="MIW95_003469"
     CDS             complement(5887..6234)
                     /locus_tag="MIW95_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152765.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(6228..6629)
                     /locus_tag="MIW95_003470"
     CDS             complement(6228..6629)
                     /locus_tag="MIW95_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152766.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            complement(6639..6872)
                     /locus_tag="MIW95_003471"
     CDS             complement(6639..6872)
                     /locus_tag="MIW95_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152767.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
BASE COUNT         2033 a         1377 c          650 g         2834 t
ORIGIN      
        1 gaagttcata atttaaacaa ttagtaatta tttaaatttg tggaacttga ttttaaaatc
       61 aaattcccag ccacttttac tcttgccacg agttgagtgg ctttttactt ttagaaatac
      121 tttacaaatt aagcaaaata ttaaactagc aataacgcca gctattacat taagtaaaaa
      181 gttattcata cttcccacct cctttcatta ggaagtaggt tttatcccag tatgaactcc
      241 actctataaa ttgtagatta catcttcttg ctaaaaatat tataacatat aattattaca
      301 tattttacct attctatatt tattttttta ttttgctatc ttcttcgtcc cctctttctc
      361 tctctttcag cttctttcat tgcttcctct tcatcttcta tctttataag tattgaggcg
      421 gctgctaacg ctctctcatt aacttctaaa ttcatatatt cacttggctt ccactttaat
      481 ttttgaatac aataatgagt gatgctagca tcaaaatcgc cacctctgat tagttttttg
      541 cttcttctac tttatcctca aaagatgtat caaatccatt gacttcattc acttttactg
      601 tataattgac atactcacct gctgtaagca ttgtctttaa taactgagct tctcccatta
      661 ctccataact attttggagt tcggcatcct ttaaatctgg aaatactgta gatgctacac
      721 ataattcagc tacataacta ttgtagtcaa tttcacttgt atattgtcca gtatgcttac
      781 cattgttacc aatcactttt actcttttag tacactttct tcttagtgct tcgtcttctt
      841 cagatgataa aacttttaat tcccattcaa ctggtttccc ttctttatct aaaaatctgt
      901 tactcgctac atattttaca ttatcaacct ttattgcatt ttgacttaaa aaagcactta
      961 aattactcat attattctaa tctcctttta ttttaatttt tcatataaaa aatacacatt
     1021 tataacttat aaatgtgtat tttactccat acctatattt tttattactt cttccaatgg
     1081 aattttttta gtaactaatt gattataaag aaatagatac ttaacattca aattttctgc
     1141 acattgtttt aaagtaatca tttcaccttt ataattaact ctaatactat ttcttttatt
     1201 atttcgttga acttcccaag taacccatct acaatttgat ggttcgtaat ttccattcac
     1261 attaattcta tctattgtta aaccttttct atatccattt tctatagacc agttataaaa
     1321 atttataaaa tcctctagcc attcattgca tacttctatg cctctacctc cataataatt
     1381 ataatcttta ttactcttat ttgtgcacct tttaatcata cggtgataaa ctctatataa
     1441 gctatcatta gatttattat gcgttgtaga gcgctctagt aatatttcct ttcttataca
     1501 gccacaactt tgtgcttttc cttgttctaa tgcacttgta ctagttattg ttctatttcc
     1561 acattcacat tgacataacc ataaattgtg actatattta tctttcccag acattttcaa
     1621 tactgtaagc ttcccaaaag ttttcccagt tatatcttta aatctcttat tttgtccttt
     1681 ccagtttgga gaacaaccac aatgtggagg cttctcattt ttaattgatt gctttaaatt
     1741 attttctact ctctcaactt tattcccaca ctcacattga catagccaga cttttttact
     1801 atgtttattt ctccctaaat atttaattaa agtaagtttg cctaatttaa ttccactttc
     1861 aaattccatg aaatacctcc aaataattta tacttacatc ataatatttt tcaagtataa
     1921 ttcaatttta taacattcca ttaagtaatg caaaggattc aactaattcc caatcctcaa
     1981 aagtgaaatc catatcttca tctaaatact caccatcagc atcaaattta gtaattattc
     2041 cactgtccat attacaatct ttaagtacta ctgtctgtct tcctacagca gatgtagggt
     2101 cttcatttgt aacttgtatg tcaaaataaa tatcctcacc agtttcttta tatctgtaaa
     2161 gtaattctct aaaaatagaa gtattataat gaaatgttgc acttccagta tttgtactcc
     2221 cagttgtttt atttcccttt gttgttcttc ctagaattgg aacttcactt ttattttttt
     2281 ccattttagc ctctaaatct atagcttgca taaaattata tcttttacct tctatagtta
     2341 taaaacattc agctttcttt gcactaactg tatctttagc atttattgtt tgagccatat
     2401 tatcactctc ctctctaatt aactgaaaca gtcatataaa gcttactcat agcatttatt
     2461 accttaatag catcagatac tatgacagtt ttcttatcat ttccaagctc tacactaaca
     2521 tcatcagttt taaaatcttc tattgccctt atattctcta attctttatg gtgtttaaca
     2581 acatcattcc agaaacttat tcttcctgcc ttatcattcg gaactttacc taaatacttt
     2641 tcattaaata aagttgcaat atcattagca atttggtcaa gtactctaac actttggtta
     2701 cttgaaaaat cgtcattttt atcatctgta aatgatacaa aagtatttat atcctctaac
     2761 acatgaactt catctcccac cttgtggaat ataaatttac cagtttttaa agcttcttca
     2821 agttgtattt gtgtataatt aacatcaaca tcaaactcac catcatactt tttattagta
     2881 ttagatttat ttatatcgca tcctgctata gctccagtag cccaataaat caagctagat
     2941 tcaactaaat ctttatcttt aatcttattt tctatagaca ctacaccttc ataatctgca
     3001 tcacttttct tatatagtac tgtttgaaac ttagctccta ccttatctct cattctcttt
     3061 gtaaattcta caaataaact tttaatttct gttgttgtag ccaaacaccc tagtgcatta
     3121 aatgaataac tttctatttt atccaagaaa gcttggtact ctgctcctgt cacagcttcg
     3181 ccattggttc caccagtaaa tacaagtcct gcacttgctt ctagtgttgc atccttcttc
     3241 caagtgatat agtcattgtc ttgtaagtct gtaataacct ttgctatttg agtatctacc
     3301 ttcttattat ctaaaagtat tacaacatca aacttagcat tatcatctat atttgttgta
     3361 actgttactt ttaagtcatt tcctcttata ccactatatt ttgctgtggc tatagtacaa
     3421 ctggctttaa cgcctttatt taatttataa aaatatccca accttatatt tttgaataaa
     3481 tctctcaaac ctttcagctt ctcatgagta taatcatatc caaaatactt cactgaatac
     3541 ttctcaaaat catcactggt tacttggaat gcttcttcat ctatgcccca atctaactct
     3601 aaaggcattg caacaatacc tctatctgat aatgaactgg ttgccctctt agctgagata
     3661 aaatttatat atgcaccagg aagtatttta ttttgcgtta cgaatgttcc tccacctaaa
     3721 gccatctaac tcactccttt cataaattta tttattctat cctctacctc tgagaaggaa
     3781 tataactcat tttcttttaa aattgcattt aataaatctt ttctatttac atacttctta
     3841 gagttaacta tctgctcctt agtaaacttg tagttatctt ctttgcttaa tgctttactc
     3901 aaaattatca cctctcttca aaccaccgaa taactctact gtatccatct tattggtatc
     3961 attatttttt atagtaaaat agttataatc aacaaagaag tgaagaacat tgtctataat
     4021 ttcaaaattc atatttgtac ctcttactaa atctccatca atttctatat actctaattc
     4081 ctccagtagc atctcagcta tctcatttat ttcaaaattc ttatcttctg aacgagggaa
     4141 ataatgtaca tcaaaagaat ttttctttaa ttctctcccg cttggatatg gtgtcttgct
     4201 tggatttaaa ggaacaataa aaaaacaagg ttcattaata ccttgctcca catcttcgct
     4261 ataaattgta tatttttctc caaacgattt atctaacttt actgatattc catctataat
     4321 attattaagc atcaaacact cctttaagca atattaatag ttttttctct attatcttat
     4381 caacttgact ttgtagttcc atttctgaaa tagttaagaa atgttgtcct ttaacccaac
     4441 ccttgccatt tttagttcta tgaccaaatt caacatatgc attttgttat cctaaaggct
     4501 ttttatcctc tagctcttat agtttcctat aagttcggcg tacatcatca acaaaataaa
     4561 ctttatttag ttgcccagca ctcttggaga gattatattt attcactctc tacgctctac
     4621 ggaaacctat agcctatccg caatctatag gttttcctcg gtattggcat atataattaa
     4681 ttaaatattt ttccatgttc ttctatggac tatatttgat atagatgaat atgttacagg
     4741 atatatttca cttagttgtt ttattgtata tccatcagaa tacttttttc taatttcttt
     4801 tacatcctca atagataact tagctctgct ttcttcaata atcttaactt tttctataag
     4861 cttatttcct actaatccct tagaatatct acttcttaag caagagtatg aaatacctgt
     4921 tttttcagaa agctctatca atgtaatttc tttttcttca tgttttacta atatatttgt
     4981 tgacctattt ctacattgtg tctttatatc cacccatcta caattgcttg attcataatt
     5041 tccttcatta tttattctat caattgtaag tttctctgaa tatccattac tcaatgccca
     5101 atttgcaaaa ttatcatagc aaaaccattc atcacacaca attatattcc tttcaccata
     5161 tctattgtat cttttattat ttttatctgt acatctagat attataccct tccatatact
     5221 gtaaagtctt ttattttgaa ccttatattt ctttttaaat tggtatttgt ctgttaaatt
     5281 taatttatct tgttcttttt ttaaacatcc acaagattgc actaagccac tagttaaaga
     5341 gtcagttctt atttctttga aatttccaca atcacactta cataaccaat atttcctatt
     5401 tcttttaccg ctttttatct ctttagaaaa tttaatgact cttagccttc caaatttttt
     5461 tcctgtaata tctaaaaatt ttgccataaa atcacctctt ttatacatta taaatcaaga
     5521 gttattctat gtccatattc tacctacttt ataatgtaac actattacac ttagcgttta
     5581 ccgattttgc tgggttttat atgccccatt gtgttaaggc atattcagtt ggattaacaa
     5641 cttctataat ataattatta ccttgtttgt aaacaggaag cgacctagca taagctactc
     5701 cactccaacc ctctctcaaa aatcctgtat caactggtgt ccttctaatt acttttccta
     5761 agagtcttgc tgctaattct cttgctgcat ccttgcaaaa cttatctaaa tcaatctttg
     5821 taaatttctc catcttttta caaactcttt taaactctct aaaatcaaca ctgccccatc
     5881 tagccattat gctttatcct taaataactc aagtattatt tcttgatgat ttggatatat
     5941 agctgattct ccacttctta catactcttt atcatttata ataagttttg aacctgcttt
     6001 aatttctata tctggagata taaagagttt aatagtttgc tctagcttag ctaatttccc
     6061 ttctgtagca gaaactatat ttttatatga aagtttacat ggttgatttt ctaatacaat
     6121 cacttcttta ttgttagttc gttttgttac agggtcttta attggctgat actcaactat
     6181 agtacattta tctctatata acatttctat tgcttttcta gttttactta ccatcttaag
     6241 cacctaaagg ttaatatctt attcttacca taagtagtaa gataagctat taagctatca
     6301 aagcgttgtt ctggtgtttg agaaccactt cctatagcaa aatctacctt tgtatcacct
     6361 tctgatatag acttttctac agcttcaaag ttaatgcttt ctatatctaa ttgacccata
     6421 tttttcttgg taaataagaa ctctccaact atcatatcaa cttcaatttc tttcaattca
     6481 attggcatag tttttatatt acaatctagt ttaataatat tttctatttt ttctcttaca
     6541 aaacctatta accacttatc tccatctttt aatatatatc caaaactttc aagtcttttt
     6601 tctatttcat caattatatt attttccata attttcacct actttttagt aagtttattt
     6661 ttctctttaa gctgcttatt ttcttcttct aaagactcaa cttttgacct taaaatatta
     6721 ttttcagcta ttaaatcttt tacacttaat gacttgccat actttactgc cttaccagtt
     6781 tcatctatca aatcatatcc catctctaag aaatcatcta ttttacattc ttctatggtt
     6841 agtattctat ttagtttttt tacttgtggc actatatatc actccttttt ctat
//
LOCUS       DAGQWR010000179         6834 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.182, whole genome shotgun sequence.
ACCESSION   DAGQWR010000179 DAGQWR010000000
VERSION     DAGQWR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.182"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            109..258
                     /locus_tag="MIW95_003472"
     CDS             109..258
                     /locus_tag="MIW95_003472"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152768.1"
                     /translation="MEIVAVYYIAKFLLDLYKVKSHSKSEAKSGFIFNLTINKLIVKI
                     KYKKY"
     gene            complement(492..668)
                     /locus_tag="MIW95_003473"
     CDS             complement(492..668)
                     /locus_tag="MIW95_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152769.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(723..819)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1030..1419
                     /locus_tag="MIW95_003474"
     CDS             1030..1419
                     /locus_tag="MIW95_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY3152770.1"
                     /translation="MEQQEVVNKLRRGELMLMEYLWRKKSILSKKEIIEAMKDRYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFELNYEVLVTKKEYLNARKEDRQVTKYDSFFIRMF
                     TTMHKKEKMTEEQIKKYIENMEKIGKE"
     gene            1499..1882
                     /locus_tag="MIW95_003475"
     CDS             1499..1882
                     /locus_tag="MIW95_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY3152771.1"
                     /translation="MGFKKLPDSELKVMKFIWGLDKNKVASGEVVEAMKQIYDWNKNT
                     TLTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTAL
                     NDEEDLSDDKLDMLEQWVKNWEEDE"
     gene            2561..3991
                     /locus_tag="MIW95_003476"
     CDS             2561..3991
                     /locus_tag="MIW95_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY3152772.1"
                     /translation="MILNQIFIEDGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGEYISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKNNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(4139..5014)
                     /locus_tag="MIW95_003477"
     CDS             complement(4139..5014)
                     /locus_tag="MIW95_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY3152773.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETSLFDRLPRGVCLTRCGISLLDEARSILTACRNLDKKISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSEQTLSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFELIKAAEAGLGITILPENLLLDSLLHKKLRLIELDEIDMENKMLAILHKDKNI
                     TQPLEIILDNISNVL"
     gene            5134..5697
                     /locus_tag="MIW95_003478"
     CDS             5134..5697
                     /locus_tag="MIW95_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBY3152774.1"
                     /translation="MNQSRKIYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCTAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSTMIPIAFFANFVM
                     SINVIFILSVCCFVCVMQVFYKKRENK"
     gene            5694..6260
                     /locus_tag="MIW95_003479"
     CDS             5694..6260
                     /locus_tag="MIW95_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBY3152775.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     ILLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(6389..6625)
                     /locus_tag="MIW95_003480"
     CDS             complement(6389..6625)
                     /locus_tag="MIW95_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152776.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
BASE COUNT         2606 a          792 c         1087 g         2349 t
ORIGIN      
        1 ataattgtaa aattatgtta taatttatat gtaaggaact aaatatctaa gagtggcagt
       61 ttccacatta tgctctaacc tactttctaa tgaaaggagg tgggaagtgt ggaaatagta
      121 gcagtttatt acatagcaaa gtttttactt gatttataca aagtaaaaag ccactcaaag
      181 tcggaagcta agagtggctt tatatttaat ttaactatta ataaactaat agtaaaaatt
      241 aaatataaaa agtactaaat tttttcggaa actacactct cgacaataga ttatagttcc
      301 ttatttctaa agaggtagga gaattacagt tctcttatct ctttttgata cgaatttaca
      361 tctttattat atcgcatttt aacaaaaaat aaaactaaga atattataaa tctattttaa
      421 ttattttttt atcatatcat tatttaaaag tagataaaac taaaaagggt gttctagaaa
      481 ataatgtcta tctaattata cttacttacc aataccatta cttgatgtaa gcaagactct
      541 actacatttt ctccatactc tagtataaaa tcatctagtt gtgtatctat aaaatcacaa
      601 tagtcatatg aactaccaat ataagcagtc aacatgtgtg tgcatttatc cattttatct
      661 ctaaacatat atattattat ccccctaaaa aactttcata aaagcaaaaa gaacaatatc
      721 tttttggcaa ctggctgaca taactcataa gatgctatat aagtcttagt ccctatagct
      781 ttgcgtcact aaatttctct agttttgccg atttagtttt actctacaac tacaataata
      841 cattagaatg agtcattatg caacatgctt gtttgaaaat taaataaatt tatagatatg
      901 aataatattt tttgtatgta aaaataaact aaaaacgtag aatgcatggt aaaacttacc
      961 tgtagttttt gtcctaaaaa actcctatta ttaacttaaa ttaagtttaa tttagggaag
     1021 tgacataaaa tggaacaaca agaagtagtt aataagctgc gcaggggaga actaatgtta
     1081 atggaatact tgtggaggaa aaagtctatt ttatctaaaa aagaaataat agaagcaatg
     1141 aaagataggt ataagtggag gaaaagcact acagaaattc ttttaaaaag attagtaaag
     1201 atgaaaacat taaaaaagaa gaggataggt tttgaattaa attatgaagt gctagtaact
     1261 aaaaaagagt atttaaatgc tagaaaagaa gatagacagg taactaaata tgatagtttt
     1321 tttatacgaa tgtttactac tatgcataaa aaagaaaaga tgacagaaga acagataaaa
     1381 aaatatatag aaaatatgga aaaaattggt aaagaataat ttatatatat taattatttt
     1441 taaaataaat ttgttataat atttaaacat acataaattt acaaaggggg tgtggcttat
     1501 gggattcaag aaattgccag attctgagtt aaaagtaatg aaatttattt ggggattaga
     1561 taaaaataag gtagcatcag gagaagttgt ggaagcaatg aaacaaattt atgactggaa
     1621 taaaaataca acattaacaa tattgtctaa gcttgcaaag aaaaactatt tgtattctca
     1681 aaaaacaagc aagtgtacat attatactat cgctgtaagg gaaaaagatt atttaaaagt
     1741 tgaaacaaaa aaatttttta gtttttttca taataattct ttaaaaagtt ttttcactgc
     1801 tttaaatgac gaagaagact taagtgatga taaattagat atgttagagc aatgggtaaa
     1861 aaattgggaa gaggatgaat aaaatgaatg tgatactaaa atgatactaa tatgttttat
     1921 agcgtgttaa aaaatataga aataatagaa ataatagaag aaaattcatg taatagatga
     1981 ggttttatca gattaaaaag aaaaacaata gaatgggaat aaaatataga atgccagaaa
     2041 tccacatgaa tacaggattt gaggtctctc ttacttctat attatactgg tttgatacta
     2101 tcaatatttg ccagtatctt atggattaat aagcaaaaaa catcctgctg aatggtaaat
     2161 tcggcggggt gtttttattt tgttttgaaa ctcctttgct ctgaaactcg aagtatttaa
     2221 cactgatgat gttgttatct tttagttcct tttgtgtcat gctctgaaag acaaattgat
     2281 acaataaaaa agtaatttct taatcattga tggagatatg tatttatgtt actcttgatg
     2341 gtaatttgag taatattgtt catagctttt tatattcact taattagaaa accatttggt
     2401 ataatcataa ataaacttga tgatataata gaaaagattt ataattatta gaagtatata
     2461 aaagtatttt atgtctatga aatgtatgtc tataaaacat atagatgact tttgtatatt
     2521 aatgtatgaa tattgatatt atagaagaat tgagttgtca atgatactaa atcaaatatt
     2581 tatagaagat ggagggatta tgagaaataa aataattata ggaattactt tttcacttgt
     2641 attactaatg tctggatgtg cttcaaaaca aacagcagaa ataaacgtat tagcaactac
     2701 tgatttacat ggagttatac cttatgagtt aacttcttat gtaaaagaag aaagaaaaaa
     2761 agataaaaat ctaactttag tagatgcagg agattttttt gatagtggag aagtgtttgg
     2821 ttctagcatg gatagttatt ttagtgctag atataaagat ggtgataata acactgaaag
     2881 ttatattgag acgcctattt caaaagatat gaaagaggta ggttatgatg ttgcagttct
     2941 tgggaatcat gaatttatat ccaacaataa attttatcta gataatatga tatctgattt
     3001 tgagaaacaa aaaataaata ttttatcggc aaatacgtat aaaaaaaatg gagatagtta
     3061 tacaaaacca tacataataa aaaatattaa tacacctgaa ggaaacgtaa aactaggaat
     3121 tttagggctt actataaaag aagttggtga gaggaaacag tggaaagatg gaaaacttgt
     3181 tgactctaag tctttagaac taaaagacca agagggctac aatggtgaat tgtatatgaa
     3241 tgatttagta gaagatgcta aaaagtgggt tggtataatg gaaaaagaga agcctgacat
     3301 tatagttgct gtggcacata caggtgagaa acctaagaaa cctagaaacc caggaaatag
     3361 aatacaagac ttagctcaac aagttgatgg aattgatgct atagttgcag gtcataatca
     3421 tgttcaaatt gagcaacatg attataaaaa taaatcagga gaaaatgtta tagttactga
     3481 gccaggaaag catggggaat atatttctaa gattaatttt aaattagaaa aaaacaatga
     3541 taaatggaat atagttgaca aatctagtaa attaattaaa tttgaaaaaa atcaagaaag
     3601 tgataaattt ggagaattta tttcttcaat taataaagta agtaaagata agaaagaaat
     3661 aagattatca gaagtaaaga ctcctgaatg ggataaaata tatgttttta aatctggaac
     3721 atctgcagat gatatatata aaacaatagg atataaatgg agaaatatat catctccaaa
     3781 gggtgatgac atggtgcaga ttgttgtaat gaataataat aagcctgtta gctatatata
     3841 tggtgaagct gaagttatgc caattaatat aaaatgtgat gaatcagagt ataaaaataa
     3901 tgttgtaact atttatccta ataaaaatga taaatttaaa attgaaaaag gtaagcaaga
     3961 atatagtatt aatttaaaac atatacaata gaaactttta tcttatttaa gtttagattg
     4021 tacttaaaat aaagtgtgta ttattttaaa aagtgatgat aattaaaatc acaacattaa
     4081 ggaatagtac acacttttta tgctaaaaaa tagaatgtta aaaaattaaa cctttaaatt
     4141 aaagtacatt gcttatatta tccaatatta tttccaaagg ttgtgtaata tttttatcct
     4201 tatgtaatat tgctaacatt ttattttcca tatctatctc atctaattca atcaatcgca
     4261 attttttatg taagagtgaa tctaataata aattttctgg caaaatggtt atacctaacc
     4321 ctgcttctgc agctttaata agttcaaaag aattaacact ttcccaaaca ggataagctt
     4381 tttggtttga aagagataat acactgtcca aagtatcgcg aatagcactt ccttgttcac
     4441 gtaacaataa cggaaacgtg cataactgtc gtagagataa ggtttgttca gataaaggaa
     4501 aagttggagc acatgcaaca cataacttat aggaacctaa taaaattgta cggaatgccc
     4561 cctgtggttc tataccttcc caaaaacata tatcagcttc tccacttttt aaaatatcca
     4621 tagctgaatt agcactttcg acacgtacat tcactttaag agatggaaat gaatttttta
     4681 gtcgacttaa aattggtgga agtaaaaaag aggcaattgt aatgctagaa acaatgttta
     4741 taggtgtaca ttcttctaaa cgactgattt ttttgtctaa attgcgacaa gcagttaaga
     4801 tactacgtgc ttcatctaaa agagaaatgc cacagcgagt aagacatacc cctcgtggta
     4861 atctgtcaaa tagtgatgtt tcagcttgtt gttcaagttc agctatagca tgagatacag
     4921 cagattgtgt gatatgtaat ttttttgcag cacctgtaaa agtgcctgtt tgttcaattg
     4981 cttctaaaat ctctaaatat cgtatgttta tcataataaa tatcctcact atacatgaat
     5041 aaaattaatt aattccatta aattctatca ttttacagat tgcaaagcaa caggtatact
     5101 gaataatatg aagtgaaaag aaggaggaat attttgaatc aaagcagaaa aatatatttt
     5161 tggcttttaa ttattaactt gtttattagc acattcactt ttggcggagg gtatgtagtt
     5221 gtacctatga ttagacgtta ttttgtaaat caaaagcaat tctttacaga agatgatttg
     5281 atgagtatgg cagcagttgc acaatcaact cctggagcta ttgcaattaa tttatctgct
     5341 ttggcaggtt atcgagtagc tggtacttta ggggttgtca ttagttgtac agcagctata
     5401 atacctccac tcgttattct tggattagta tcagcatttt acaccacttt tatatctaat
     5461 gccattgtaa ctgcagtatt aaaaggaatg caagctggtg ttgctgcact aattgtggat
     5521 ttgattattg acatgtgctc tatgatatta aaacaacgtt ctttgttttt atcaactatg
     5581 atacctattg ctttctttgc aaactttgtg atgagtataa atgtaatttt tatattatct
     5641 gtttgctgtt ttgtatgtgt aatgcaagtt ttctataaga agagggaaaa taaatgaata
     5701 caatttggaa cttatttttt atatttttaa aaataggctt gtttagtatt ggtggtggtt
     5761 atgcaatcat acctttaatc caagagaaag tagtaaatca aactggatgg ataagtgaga
     5821 aaatgtttac agatattata actatttcac aaatgacacc agggccactg gctgttaata
     5881 cttctacttt tgttggactt caaattggag ggttttgggg agcagttgca gccactttag
     5941 gttgtgtatt atgtggtgta gtaatatctc tatttttata taattttttt caaaagcatc
     6001 agaactctac ttatatattt gaaatattaa atggtctaaa atcagcttca ttaggtttaa
     6061 ttatctcagc tgcggctaca attataatcc ttgcattatt tgggacgaat aaagtgtact
     6121 tcaattctat atttgagatg ctaaactgga ctgcactaat aatctttttt gttatactac
     6181 tgatagtacg taagtggaaa attaatccta ttgtgattat aataataagt ggtatactag
     6241 gaatattttt ttatttataa gtttttggta agtaaaggat atctattttt ttatatctta
     6301 aatccaaata gccaataaag taaggctatt tggattttta ataaattctg aaaagataat
     6361 cagaaaccca tataacattt aaattatatc actggttttc tacaaaccaa tcataagctt
     6421 cttggcaaaa tttctcacta tctatatagt aactattatg aacattatta atttcaacaa
     6481 gtttcttttt acattttagt gaattataat agctatcaaa atattgggtt ttaaaatatc
     6541 tatcatgaag tgttttaaag acaagagtag gtatgttgac tccatctttt atcttactat
     6601 cttctgcaat aaagcattct aacattgtat taattatctt tgtactattt ttccaaatat
     6661 gaggattttt cttttcaaat attttataaa attcattatc atagacttca ttataacctt
     6721 tatatttagg tggttttact gttatgtgaa aattaggaag aaacttaaag aaaaaatata
     6781 ataattttta tgttctgttt tatatattat tcatatttta tcacagataa tata
//
LOCUS       DAGQWR010000180         6782 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.183, whole genome shotgun sequence.
ACCESSION   DAGQWR010000180 DAGQWR010000000
VERSION     DAGQWR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.183"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(62..1747)
                     /locus_tag="MIW95_003481"
     CDS             complement(62..1747)
                     /locus_tag="MIW95_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152777.1"
                     /translation="MKYLIEIAKKERLLVFIYLALGIGIELLNSFSANYYQSLIDRFN
                     NSTINFHIIFIYGTVLVTLCLLRYIDEYPGRRLEHSFFLDLKLKALEKMSKIDYLEYQ
                     MLGTGKLIQKIENGANAGRNIFFNFLLAVVRKIIPSILFSMVFIYRIDKKVMFTILIG
                     YFIVFIITNLLIKALYQIKEHILINEEKMNHFLVRGFVEMVVFRVNKRFEYEICQSKF
                     AKKEIIDSRVKMTLIHEAFFTIFSLIVTFIKMLIIVYSWKTKTISIGSIIALITLLDN
                     AYTPIAIFNVLYIQFKLDKAAYKRYTDILELNNDGQLLAGKEVNLMYGNIEFNEISFL
                     YNNKREIFNKLSLSIKSGEKVAIVGESGSGKSTLIKLLIGLLKPSEGTIFIDDYNLLE
                     MNLNSYYNYVTYVSQDSPIFDGTLRENIVFGKTIKSNDIIEVLEKVGLKNLYLKLEAG
                     LDTKLGEKGTTLSGGEKQRLALARLWFSDAKIIILDEATSALDNITEEKVMLNVMEFL
                     KDKTTIMIAHRFNSIKNVENIIVLKEGSIIGSGSFKKLLDGNLYFRELYYTMS"
     gene            complement(2670..3251)
                     /locus_tag="MIW95_003482"
     CDS             complement(2670..3251)
                     /locus_tag="MIW95_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY3152778.1"
                     /translation="MNNKKHWYDYLWIFSSIYLVLGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(3244..3474)
                     /locus_tag="MIW95_003483"
     CDS             complement(3244..3474)
                     /locus_tag="MIW95_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY3152779.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            complement(4799..5074)
                     /locus_tag="MIW95_003484"
     CDS             complement(4799..5074)
                     /locus_tag="MIW95_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBY3152780.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            complement(5150..6451)
                     /locus_tag="MIW95_003485"
     CDS             complement(5150..6451)
                     /locus_tag="MIW95_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY3152781.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
BASE COUNT         2333 a         1237 c          551 g         2661 t
ORIGIN      
        1 ttccagtaat atttaaataa tattaaatta agaaaaatat tttcttatac ttacataata
       61 attatgacat tgtatagtat aattctctaa aatataaatt accatctaat agttttttaa
      121 agctaccact acctattata ctcccctctt taagaacaat tatattttca acattcttaa
      181 ttgagttaaa tctgtgagca atcataatag ttgttttatc tttcaaaaat tccataacat
      241 ttagcataac tttttcctct gtaatattgt ctaaagctga tgtagcttca tctaatataa
      301 ttatcttagc atctgaaaac caaagccttg ctaatgctag cctctgcttt tctccacctg
      361 ataatgtagt tcctttttca cctaatttag tatctaaacc agcttctaat tttaagtata
      421 aattttttaa acctactttt tctaagactt caataatatc attacttttt atagtttttc
      481 caaatacaat attctcacgt aaagttccat caaatatagg tgaatcctgt gacacatatg
      541 taacataatt atagtaacta tttagattca tctcaagtag attataatca tctataaata
      601 tagttccttc gctgggcttt aataatccaa ttaatagttt aattaaagtt gatttaccag
      661 aaccactctc tccaacaata gctacttttt ctccactttt aatacttaaa ctaagtttat
      721 taaatatctc tcgcttatta ttatacaaga agctaatctc attaaattca atatttccat
      781 acattaaatt aacttcttta cctgctaaca gttgaccatc attattaagt tccaaaatat
      841 ctgtatagcg tttatatgct gctttatcta gtttaaattg aatatacaat acattaaaaa
      901 tagctatagg agtatatgca ttatctagta aagttatcaa tgcaattata gaacctatac
      961 ttattgtttt agttttccaa ctataaacta ttattaacat ttttatgaac gtcactatta
     1021 atgaaaaaat cgtgaagaaa gcttcatgaa tcagtgtcat tttaacccta gaatcaataa
     1081 tttctttttt cgcaaatttt gactgacaaa tttcatactc aaatcttttg tttacccgaa
     1141 atacaaccat ttctacaaat ccacgaacta aaaaatgatt catcttctct tcattaatta
     1201 atatatgctc tttgatttga tatagagctt ttattagtaa attagtaata ataaaaacta
     1261 taaaatatcc aattaatatg gtaaacataa cttttttatc aattctataa ataaaaacca
     1321 tactaaataa aattgatgga attatttttc taacaacggc taataaaaaa ttaaaaaata
     1381 tatttctgcc agcattagct ccattttcaa ttttttgaat taattttcca gtgcctaaca
     1441 tctgatattc taagtaatct attttactca ttttttctaa agcctttaat ttcaaatcta
     1501 aaaaaaaact atgttctagt cttctacctg gatattcatc aatatatctt aaaagacata
     1561 aagtaactaa tacagttcca tagataaaaa ttatgtgaaa atttatagta ctgttattaa
     1621 atctatctat taggctctga taataattag cactaaaact atttaaaagt tctattccta
     1681 tacctagtgc taaatatata aaaactaata acctctcttt ttttgctatt tctattaaat
     1741 atttcataac tgtacaccta tccttattta ttttacttta ttaacttaaa ataattttat
     1801 agataaaatg tttctttatt tgtttaaatt tagtgtgatt attaatcatt tcaagttaac
     1861 aaaacctaaa gtttttagca gtacagttct atttgttagc aaattattat tttactcagt
     1921 acattatgct aacatataat ctaactgtac tgagtaaata tctcgtttca tcatataaaa
     1981 tctccttttt atagtttaat atatatagtt aaatttacta tataatctga atatttattt
     2041 ttctatcacc tttaccagaa ataaaatcaa ttatttattg tataagcaaa acttcataat
     2101 attttcaaca tataattatt atacagaact cctaagttaa tttaaagtta gaatttattt
     2161 ttataagaaa tgtcattgac ctacctttaa taaaacagcc accatttttt tatgtattaa
     2221 catataatat atagtaatga aaacatatta attaaaaaat attgtataaa ttagtttatc
     2281 tgtaacaaat ccagtttatg tttcaacaag cacgtaagac ttcttgaaat ttaatctgct
     2341 tcctttaaat atctattact tgttttaagc ataagtttac aatcttctat aacatttatc
     2401 atttcttcag atttctctga cgtacttaaa atacaagttg ttacattgtt tttctattta
     2461 attagtaatg tatcatgtaa aaaagaagca taaatgagtt tactttatac aaacttcatt
     2521 tatgctccac tcatatacct atcatagcta atagaaacta ttactcatct tgaatctatt
     2581 tcatatttag atatttatat ctttcttatt ttcttaaatc aactctagct ttaaacatta
     2641 aaccccttaa atcttcacaa aatctcttcc tatacagact tactctttgc cttacatatc
     2701 atctgagtca tagtacccat agggcaataa acacaccatg aacgaggttt aaacaatatc
     2761 atagtaatca aaccaagaac agttgatgta agcataacac tataaaaacc aaatgcaaac
     2821 tgtaccaccc aaggtgaaac taaagttcca tgatacgccc attgccaagg catttttata
     2881 gtccacaaca aagtcactac ttctttaaaa tttctacttc cttcaaacac taaataagtt
     2941 gaaaaaagca tattggcaaa cattaccata aaaaatgtca aaaacccata tctaaaccac
     3001 ttacttctaa taaatttagg aatatctcta tttcttgaca gcttcaattt atttccaagc
     3061 aaattaaaca gttgccctct tccacaatac ttattacagt aagctttatt tcccttaaca
     3121 atagaaatag ctagtggaac aaagaaacaa attagcccaa tccaagcgaa aataatattt
     3181 acaaatccca ataccaaata tattgaagaa aaaatccata aatagtcata ccaatgtttt
     3241 ttattattca tgacacttac cttcttcctc ttttaatatt gttataatct caattacaga
     3301 tgctggacat atcttttcac aaatcccaca tccaacacat aaatctctat taatttttgc
     3361 actaatgcca catggaactg agatagcact tcttggacat gcttttatac agctaccaca
     3421 agcaacgcac tcatttgcta aaatatttgc atacttcttt tttttattag acataataac
     3481 ctccctatat tattaatcta taagaattat atcaagatac ttacttttta tctgtgactt
     3541 aatcacataa aacctcaata ttaaaaatca cactctaaat acaaaggagt ttcaaataaa
     3601 aaacgatatg aagtcattaa gtttagcttc atatcgcctt ttcttttgtg tatctatata
     3661 aatatttatt atctaatatt taatatatct ataatatttt cttatacagc tctataattt
     3721 tacacctaat catattctat tagtaacagg ttaataccca tctaaaacta taactttatt
     3781 cacaatctta ttctattagc cacaagttaa actcatattg aagtttatct ttagttacac
     3841 tcttactcta ttaatcacaa gttaagccca tattgaagtt tatctctatt tacactctta
     3901 ctctattaat cacaagttaa gctcatattg aagtttatct ctatttacac tcttactcta
     3961 ttaaccacaa gttaaactca tattgaagtt tatctctatt tacactctta ctctattaat
     4021 cacaagttaa gctcatattg aagtttatct ctatttacat tcttactcta ttaaccacaa
     4081 gttaaaccca tattgaagtt tatctctatt tacactctta ctctattaac cacaagttaa
     4141 gcccatattg aagtttatct ctatttacat tcttactcta tcaatcatag gttaagctca
     4201 tattgaagtt tatctttagt tgcaccttac tctattaatc acaagttaaa cccatattga
     4261 agtttatctt tagttacacc ttactctatt aaccacaagt taaacccaca ttgaagttta
     4321 tctctattta cactcttact ctattaatca caggttaaac ctattataat cttaacttta
     4381 ttcacaattt tattctatta gccacaggtt aaatctatta taatcttaac tttattcaca
     4441 tattacatca ttaatcgcat atcaagattt tataataagc tttaactaaa cacacgattt
     4501 taccttattc gtatagttta atctattaaa ttattatata atcttaagca aacatacaac
     4561 tctacactat taattatagc ccctcctttt aaatactcat aatcttatac acaaccttac
     4621 cttattaatc acacatttaa ctcatatata tcatcaattt tattcacaaa atcatgctgt
     4681 taatcacact tatactcctc tataatctta aacttataca caaattagcc acaattttat
     4741 ttcaactata ataacttcta tttcagaagt atatcacact tgtattctcc aaaaatatct
     4801 atttttttct aacaatatgc ctgacattaa taacactcat ttcaaacatc ctcgctaatt
     4861 ccatataatt tttcccatta aacaatttaa tcataagctc atttctagcc tcttttaatg
     4921 ccccttccag tgttttaata taaaaatatg ttcccccact ttgttgacac aattcaaggt
     4981 attccaataa actatactta tgtaactcct ttggaatact tacttctatc ttgtgttctt
     5041 caagtaattc tataattttc gttaattcca acatattttc acctcatatt ttataatcta
     5101 tttgctattt cctgactttt aataagcttg ttattactac tttattgacc taataattta
     5161 gttcttgaaa atgtaactta tccccaaagt aacaaaaatg cttatgccta gtctgtccat
     5221 ctctacactt cgcaactatt aaatcaacca tcttaattcc cctgtattct gatgctctaa
     5281 ctgcctcctc actttctcca atatctctca ctgcttcttc ataatcactc ccctttaatt
     5341 gatgtatata aaccacattg ttgctatcgt ggtagatggc tttgctatcc ctcataggtc
     5401 tatctccata aggtcttgaa tctttcattt catcatttaa ctgacttaat tggattacag
     5461 gtatattaaa atctaaagtc atatttttaa gttctctcga aaaagtagct acctcccttt
     5521 ctctcttatc catgcttttc tgagatgtaa ggagttgcac ataatccact attaataaat
     5581 ctggctttac ctgccttatc ctctttctaa ttgcactaat tgtatctatc ttatcattaa
     5641 tataaattaa gttattttcg cttaaatcac ctatagcatt tacaataagt ttccaatcat
     5701 tttcatctat ttctttagat ttcatattcc ttgtacttac accagttact ctgcatatat
     5761 ttctcataaa tacttgctca ctggtcattt ctctacttat aaataaagtt tttttacctt
     5821 gcttaaatga atttaacatt atttgaagtg ccaaagcagt tttcccaacc cccgatttag
     5881 ctgctattgt ggttagctct cctttaaaaa gacctcctat agtagtatca agtaatttta
     5941 taccaaattt aaatccaata tcttttttgt tttctaaaaa atcaagcacc ttccctgcta
     6001 tactatttac atcatcattt tcataagtat ccttgtccaa aatctccttt atattacttt
     6061 caaatttgta aatacaagta tcgatatttt ctccaaggtt taggctgtgt aaaaggtttt
     6121 ggcagttttg atttatgctt cttctagcta aattttcttt taaaatttta atatgaccat
     6181 caagaccaaa attctgaccc caagtaatta gattagtcac atcactcgtt tttattgcta
     6241 gagattttct atctatcttt gatttcacag tggccacatc aatcactatg ttttcttttt
     6301 taagttcttt cataatttta aatatctcaa gacatatatc attcataaac atgttttcat
     6361 ttaattcatc tagcttatag cttaatttat tgtctaaaag aattgtgcct aaaatagact
     6421 tctctatttc aacatctttt gatttaccca tataaattaa tctctctttc cttataagtt
     6481 tttatatttt gatttttaaa tacattatca attataaata cattatttgt tatgactcac
     6541 tatttgttat gactatatta ttcgtcatga tatactacaa attattaata tactatttat
     6601 tctgatacat ataatttact acataatagc aatatcttat ttgatataac ttactaccaa
     6661 ttattaatac attgttcaat ataattcatt actcgatagt aatatcttat ttgatataac
     6721 ttactgccaa ttattgatat atcgttcaat ataattcact acctgatagt aatatcttac
     6781 tt
//
LOCUS       DAGQWR010000181         6611 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.184, whole genome shotgun sequence.
ACCESSION   DAGQWR010000181 DAGQWR010000000
VERSION     DAGQWR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.184"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(25..633)
                     /locus_tag="MIW95_003486"
     CDS             complement(25..633)
                     /locus_tag="MIW95_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBY3152782.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(742..3480)
                     /locus_tag="MIW95_003487"
     CDS             complement(742..3480)
                     /locus_tag="MIW95_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBY3152783.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNRKFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3628..4419)
                     /locus_tag="MIW95_003488"
     CDS             complement(3628..4419)
                     /locus_tag="MIW95_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY3152784.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4455..5111)
                     /locus_tag="MIW95_003489"
     CDS             complement(4455..5111)
                     /locus_tag="MIW95_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152785.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5104..6069)
                     /locus_tag="MIW95_003490"
     CDS             complement(5104..6069)
                     /locus_tag="MIW95_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBY3152786.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEHVKQYLTSKGIRWEEMINE"
     regulatory      complement(6175..6281)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         2102 a         1109 c          602 g         2798 t
ORIGIN      
        1 tacatatctt acctatataa aaacttattc tatataaaaa cgccataaaa aatcttttgc
       61 ttcttctgca taatctatat ttatacgttt actagttttt atatctaact catctttact
      121 aaatactttt tttcctttat catcataata aaaatcactt atatataatt cttcacccat
      181 tatactctta ctatttagac ttctatctat ttttaaagct ttgcaaagtt ttcctggtcc
      241 gttagtaata ttttttactt gatatttact caactcagtg taagctttgt tgtatctatt
      301 catagatatc tcatctaaag aagttatagg ctctacccct cttattaata cacactctgg
      361 cacattttct atatttgcag ataaatttaa gcaattatac ataccataaa ttaaataaac
      421 atatatatgt ccaccatcta aatataatgg cttagttcta tctgtctttt tattgccata
      481 tacatgtgct cctttatcat ttacacccat gtatgcttct gtctctatga ttttggtaac
      541 aattacttta ttttcatatt tccttattag atatttacct aatattgatt tggctaaatc
      601 aattccattt tttctaaaaa aatccttttc caaaatttca cctcataaaa tttatttaaa
      661 acgcatcaaa tacaactaaa gtaataaata aaattaactc ttattaatgt gatatatata
      721 aacaataaat ttagccattt attatttata tatatcaaaa aattctatct caaacttttc
      781 aatattatca ttgtaatttg aattatcata tacttcttca acaacctctt taattgggaa
      841 tataactttt acattagttc cttctcctac cttactatct atgtaaatct tcccattttg
      901 aatttcaatc aatgattttg taaggcataa acctatacca cttccaaatt catttgatat
      961 accactgttt tcaatttgtt taaatcgatt aaatatatca tttaattttt ctttgtctat
     1021 gcccactcca gtatctttta taattatctc tactgtttcg tcttttttat atatacctac
     1081 aaggatttct cctccttcta aagtaaattt tatagcattt gataatatat ttagtattat
     1141 tctttctata gattctgggt caagccctac cataacagtt tcttcatccg tatcaaaaat
     1201 taaatctatt cctttagact tagcataact actaatagat gtaactattc tttctgttat
     1261 gtcaataata tcaaaattaa ctgctttaat cttatatatg tctgacttaa cttgagaaat
     1321 atttattata ttatctatta atctcaataa tctaaacata ttttgtttag ttatttttat
     1381 atgttctttt agtatatcct gaaaatcaat tatttttcct tttttataaa ggttttcaat
     1441 aagttgaact gaactatata tattatttaa aggtgtctta agttcatgag atatattagc
     1501 ataaaactcc atcttcattt ggtcatactg tctttgtttt attaatgctt ttttaagtct
     1561 ttcagactct ctttgcacac ttaaatcttt aataattctc actctacttc ttttctgtcc
     1621 aaagtataat gttcttgata tcatttttac ttctttaatg tgattatttt catttactat
     1681 atggatactc ttctctcttt ctcctatatt taaattgttt atgtattttt ttactaatat
     1741 atttctatat ttttgtgttg taactcctaa tacatcacta gaaattccga acaactctct
     1801 aaaggctcta tttgtatata gtactcttga tgtacttaaa tcctcaagaa aaatagcctc
     1861 tggtataaca tcaagtaatt tttcatatat tattttttgt tcatttactg ttctaagaat
     1921 atttatctct ttagttatat ctctaaatgt aagcaattta aagagtttat tatctgacca
     1981 atagtctcca actctaactt cttcaacatg ttcttcacct tctgagtcag acatcacaaa
     2041 attaactata cagtttgctg tgtcattgta acttactatt tcagctttct tttcttgtgt
     2101 tagatttaat cttttaaatt tcctattgtc aaagaaatct atgttaaaag tttcaaaata
     2161 ttgttctaat gtcatatcga ctaaatcact tgaaacacat ttaaaactct tttcatattt
     2221 tttatttata cttattacct tattcttctt tatgccacct tcagcacttc ttacaagtat
     2281 aggagatgga atatttagta taatgttttc atagtcttta tttatatttc ttatttcttc
     2341 atctattttg atttcatttg taatatcatt taaacttatt aagtaattgc tttcatcttt
     2401 attattactt aaacttaaca aataaatttt atcatttaac tctaccagtt tatcaattat
     2461 acttatatcg ttatcaaatt tcttcaattt ttcagcaact ttttcatctt cctttataaa
     2521 agttaatatt ttataataat tattcatatc ctttgaaaat tcatctttaa atttattatt
     2581 tgtgtaactt atattaaaat catagtctgt tactatccat acatttggta ttgtatccag
     2641 agcttgtcta agtaattctt ttctctggtc tatgtagttt tttaatgttc tttgtataat
     2701 catatccttt tctattgcta aatttatctt tcctaattgc tctgactttt tacttaattt
     2761 tttattgatt gaatcaagtt cataatatgg atacactact ctattattaa gactgtatac
     2821 atatacataa taagtatggt tacataatag taaatatatt actcctatta atctatctga
     2881 atctactata ttaatcttag aaattgctac aaaacatata atagttgtaa aaaaaacaaa
     2941 atatgttaaa tcttttatta acttatagtg ctcaatgtta ctatcaagtt tattcaacaa
     3001 atatagtaaa ttaaacatag atatacaaat attaattaat ataataatca tattccagtt
     3061 aatatcacta aaaatatttc ttataaagta atctaatatc acaaaagcac tataaaaaat
     3121 tagtattggg cttattctta gtttttttac tttagaaatg gttaagtata taaaagtaga
     3181 catattaatt atgcttaagt taagcaatac aatttggaat ttttgaattt ttaaatttac
     3241 tactcctaaa atacttacaa aaactaatac aagacaccct actttcaata tatttataaa
     3301 ttcatctgtt ttaaagctac ttatataaac tatagaatat ccaaatatcc aaagtaaaat
     3361 tattctatac atatttatag aaactgtata caaatttata tttaaaagtg caataaccaa
     3421 aaataaccca ttagctagta tatagacctt attcttactt aaagttcctt tagattccat
     3481 attccccacc acttcttcat ttttatatag ttttacctat aaatatgtta ccacaattct
     3541 tccaaaaaat ttgtcacttt ctagttataa aaaaaaatga gtaaatattt ttaattttac
     3601 tcattttgta gattttgtat attcagttta aaaagctgga actatacttc ctttatattc
     3661 atcttgtata aattttttaa cttctttact attcatagcc tctgataaag ccttaatctt
     3721 atcactatct ttattatttt ctctacaagc tattatattt acatatggag aatcacttga
     3781 ttctattact attgcatcct tagtaggatt taagttagct gttaaagcat aattagaatt
     3841 tattactgct ccgtcaacat cttttaatac tgttggtaat tgctctgcat tcatttcttt
     3901 tattactata tttttaggat ttttagttat atctttttta gttataagct ctccatcttt
     3961 tacttctatc aatttatttt ttgctaataa tttaagtgct ctagctccat tagttgcatc
     4021 atttggcaca gctataacag ctccatcttt taattcatct aatgacttaa ctttttctga
     4081 atagaatccc attggctcta tatgaacttt agatgtataa gtaagtttat atcctttctc
     4141 cttcacagtt tcctctaaaa atggaatatg ttgaaagaag ttagcatcca aagacccttc
     4201 atctaaagct gtatttggaa gtacataatc atcaaatatt tttacttcta aatcatatcc
     4261 tttttctttt aacaaaggtt ttgcaacttc caatatcttt gcatgtggat ttgatgaagc
     4321 tcctactagt atttttttat cctctttgtt tgaacaacct actgctgata ttgctattgc
     4381 tgacaccaat gctactgata ataatttctt taatttcatt tttactcccc ctctttaaaa
     4441 tgtttataat taatttattt tagtttctta taagccagat ttcctactcc ctggattatc
     4501 tgaactaata ttataagtgc tattacagta tataccatta ctgctgtatc aaatctttga
     4561 tatccataaa taagtgctat attacctaat cctccagctc ctactgcacc tgccatagct
     4621 gtataaccaa gtatagatat tattgataat gtaattccag atactattga aggcatagct
     4681 tcctttatca taactttaaa tactatttgt ctttttgtag ctccaaaaga ttttgctgct
     4741 tctatcaatc ctttatcaac ttcatttaat gagctttcta taattctagc gataaacggt
     4801 gctgccccaa tagttattgg cactattgca gcagtttccc ctatacttgt tccaacgatt
     4861 aatctagtta ttggaatcat tgcaactata agtatcataa atgggaaact tctaaatata
     4921 tttactataa atcctaatgc actatttatt gtgctatttg gttttaatcc atctggtttt
     4981 gtaactacta atatgattgc taatataaat cctaatattg ttgccactat agttggtact
     5041 ataaccatat acaaagtttg taacaatgca tttggaaata gtgttgttaa aaagtcaatt
     5101 aaactattca tttatcattt cctcccatct tatgccctta ctagttagat attgttttac
     5161 gtgctctcca cttttatctg aaatgtttat aataagtgaa cctaaaatat catctttaaa
     5221 ttgttctaat ttcccaaata ttatagacac atctatattt agttctcttg ccatagtcgt
     5281 aataatagct tcattagaaa tatctttagg gaatagtatc tttatatttg ttccttttgg
     5341 caatattata ctttcttcac ctattaattt tctaagtcct ttagtatttc ttaagaatat
     5401 ttcttctgta tctcctacct ctaatacttc tccattttcc attattgcta ctcttccaca
     5461 aatctgcttt ataacttcca tttgatgagt tacaactatt attgttattc ccaatttttt
     5521 atttatatct tcaagcaatg aaagaattga ctttgtagta tttgggtcta atgctgaagt
     5581 tgcttcatca caaagaagta cttgtgggtt tagtgctaaa gctctagcaa ttgcaaccct
     5641 ttgtttttgt ccgccactta gatttctggg tttatcattt ttcttttcag atattccaac
     5701 tacttctaat aactctaaaa ctcttttttt aatttctgcc tttgagtatc caaaacattc
     5761 taatggcaat gctacattat caaatacagt ttttctttca agcaatgaaa agtgttgaaa
     5821 aatcattcct aactcttttc ttaaatctct catttgcttt tcattcaatg actttacttc
     5881 tttatcagaa acaagtacac tgccttcttg gtattcttct aatccgttta tacatctcag
     5941 tagtgttgat ttccctgcac cactatgacc tattattcca aaaatttctc cagattctat
     6001 ttcaatactt acatctttaa gcacttgaat ctttccataa tacttattaa cattttttat
     6061 gctaatcata aaatttcctc ccatatattc tgctcagttt ttatgtttat cctctcttat
     6121 gtattacaac aaaataaaag cccgagtata aactcaggct tagaaagcta tttatactca
     6181 tctatcaact atattagttg caggatttag caccaatgta tataaaatat actggttgcc
     6241 gggtttcaaa gggccagtcc ctccaccact cttgataaga cgttatttaa ttcaattcta
     6301 ttatcatttg ttgctatgtt atatatgata tattcattaa tagtatttgt caataaattt
     6361 atttaaattt tttatttttt attatcgtta attaactatt tttaattagt tttctagctc
     6421 ctaacacttt atattttaat gtctatataa ctatagaaca tcattataag aaataaatta
     6481 aataacatca atctatctaa atcatctgta aagtaaatta agaaaattaa cattttatcc
     6541 aaactctatc ctactattaa ataaaataat ttaaatacaa tgatgattat atagctctaa
     6601 tactttagac t
//
LOCUS       DAGQWR010000182         6578 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.185, whole genome shotgun sequence.
ACCESSION   DAGQWR010000182 DAGQWR010000000
VERSION     DAGQWR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.185"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            320..1201
                     /locus_tag="MIW95_003491"
     CDS             320..1201
                     /locus_tag="MIW95_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBY3152787.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSNKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1583..2386
                     /locus_tag="MIW95_003492"
     CDS             1583..2386
                     /locus_tag="MIW95_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152788.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            2398..3528
                     /locus_tag="MIW95_003493"
     CDS             2398..3528
                     /locus_tag="MIW95_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY3152789.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLRESIDEPIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEGFVKSYFESYSLIRG"
     gene            3532..4941
                     /locus_tag="MIW95_003494"
     CDS             3532..4941
                     /locus_tag="MIW95_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152790.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDMDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDVEIDYSP
                     ERTHDIWDESNEIDKNTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            5152..5661
                     /locus_tag="MIW95_003495"
     CDS             5152..5661
                     /locus_tag="MIW95_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBY3152791.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLSEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         2696 a          573 c         1133 g         2176 t
ORIGIN      
        1 ttttttgtaa aattttaaat ttagagacta acatgagacc acaaagagga agtttattat
       61 aagaagaaaa cttaaaattg attttaaata gcaaatgaaa ataatcaaat atgtatatac
      121 tattattaaa aaataaaatt atatagttaa tataatttta taattggttt agtatagaga
      181 tttttaactt ttatgttata ctatttataa aagtaggtat aaagattaaa agacataatt
      241 aaatacgtat gagaggtttt aaatagtatt gttaatgaac ttgattattg tttataatta
      301 caaattggag gagataaata tgaaaattac tgaaggatat atgcctttta aaggttttaa
      361 aacgtattac cgtatagtag gagaaaatac ggaagggaag aagccattag tattacttca
      421 tggaggtcca ggttctacac acaactattt tgaggtattg gataagatag ctgaaagtgg
      481 tagacaagta ataatgtatg accaaattgg ttgtggaaat tcctttgtag aagggcatcc
      541 tgagttattt aatgcagata cttggataga ggagcttatt gaactaagaa aacatctagg
      601 acttgatgaa attcatctgt taggtcaatc ttggggaggt atgcaggcta tttggtatgc
      661 tattgaatat aaaccaaaag gtattaagtc ttacatcctt tcatctactc tttcttctgc
      721 aaaactttgg gagaaagaac aaaaaagaag aatatcatat atgagtgagg ttgaccaaaa
      781 agcattgctt gatgcagtaa atactggaga ttactctaat aaagaatata atgatgcatt
      841 agaaagattt atggaaatgt attgtgcagg tgaagtgaca gaagattctc cagagtgcct
      901 gagaagacct aaaaaatcag gttctgaagc atatattgta ggatggggac aaaatgaatt
      961 ttctcctaca ggaactcttt ctggatatga atttacagat agattacatg aaataaaaga
     1021 gccttgtcta gtaactagtg gagcaataga tttatgttcg ccatatattg caaagactat
     1081 gtatgataga atacctaata gtaagtggga gttatttgag tattcaagac atatgccatt
     1141 cgttgaagaa aatgagaaat atataaaagt cttaactgag tggttgaatg cgaacgatta
     1201 aaaactatat aaatttatat aaagaaatga gtgtctcaaa atgaactttt tagttcatta
     1261 ggcactcttt ttgcatgaaa tcaaatatat tttcattatt tgaacgataa aatgaggctt
     1321 atatctattt tgggaaagta attttttatt actagcgtta gattctaatc taattgtata
     1381 cttattgaaa taatagaaat atattggtgt ttataactta attttattgt tttttagaaa
     1441 tttttttata tattggtata attattctgt ttaaatttga ttaaaaagta tattaattat
     1501 aataagaaac aattatataa ataggaatag attattataa ttaatataga tataaaaata
     1561 attatagaaa gaaaggcaca ttatggaaaa attgagagtt attgaaatga taaaaaataa
     1621 tcccaatatt atagctacaa tagataatcc aactgatgaa atgaaattgt tagctattaa
     1681 agaaaatggg cttgtactag aatatataaa taatccaacc agagagatgc aagaactagc
     1741 aatagataat aatattagag caattaaatt tatagataat ccaacagaag atatgatgat
     1801 aaaagcagta aatgagggat ggagtatact ggattatatc aaaaacccaa ctgataaagt
     1861 tattgaaatg gctataaatc aagcaggatg ggcaattaaa tatgctaaaa atccaagtga
     1921 agaattacaa ctgttagcag taagaaaaaa ttatgattcg ataaagttta taaaacaacc
     1981 ttatgaaagt gtacaagagg aggcagtaag aataagttat gatgccttga gatatataga
     2041 ttcacctagt tataatgcag aacttatagc tataaaaaat aatgaagcag ctattagctt
     2101 tataactgat ttagataaaa ataaaatgtt ggaattttta aaagtgaata ttttagtgat
     2161 taagtacatt ctgaaaaaag tatctaaaga tgaattagag agtgtattaa aagaagtatt
     2221 atccaaagaa gatgttgagg aaaagtatat tagagatttt ttgaattgta gtgttataga
     2281 tagaaatagc gaaagtatac caatagataa aattatgttt atttataaat atggaagtaa
     2341 gacagcaaaa aaaatagctg tagatgagaa attaaagatt atgtaggaga gagaaaaatg
     2401 aattttatag acacattaaa aagcgttgac ttaatattat ccactggaga agtaccttta
     2461 atagtaggtg aaagtggaat aggaaaaaca gctttagcaa ataagcttgc caaagaaaat
     2521 gattggagtt taattgttat tgatggaaac ttacttaaag agggtgaaat aggtggtctt
     2581 ccaactatag aatcttatgt aggagttaat tctaatggat ataagactga aaagaaaaca
     2641 acagtgtatg ctgttcataa caaattaaga gaaattgatg aagagatatc taaaagtaag
     2701 acagttcttt tatttataga tgagataaat cgttgtgagc atacagtaca gcaagaactt
     2761 atgaatttaa ttttaaatag agaaattaat ggatataaac tgcatgatga tgtaaagatt
     2821 ttagcagcaa tgaatccatc aagtaaatat ggttcagatt ttgattatca agtggtagat
     2881 atggatgcag cccaagaaaa taggtttgtt tggttaaata tggagagtga ccatacacaa
     2941 tggataaaat gggcaataga tgagggtatt gaaagaaaag ttatcgagtt tatttccact
     3001 tttccagaat atctgcataa aataaatgaa gatgatgtaa gagctactcc tagaagttat
     3061 gagagagtat ctaaaattta taaagtttat aaagaaaaaa ataattctat tcctagagca
     3121 gtatttttaa atgttgtaaa gggtaatgta ggtaaagtta ttgcagaaga atttataagt
     3181 tttattgaat caaactctga accattaata tcctatgaag atgttttttt aagagaatct
     3241 attgatgagc ccattgtaga aagagtaaaa aatgagagtc atacaagact ttatctatca
     3301 gctatgaaca ttctaaaaga cttagaatta aatattaaga atgataaata tgaatcaaac
     3361 cactatatta atagatttat tgaattttta aaaatgtatc ctgtagattt aatgataggt
     3421 attatgaagg atattagaaa cagttatatt gaagtatata aaaaagccat agaaaatgaa
     3481 ggttttgtaa aatcatattt tgaatcttat agcttaataa ggggataatg tatggaaaat
     3541 tattttgata aacaagcaaa agagctttat aataaagcaa gtgaaattat aaatactcat
     3601 tctatgctaa aggcaaatcg tgctaatgag aaatttgata tggatatacc acaagatttt
     3661 aagtatgagt tttttagtct tgtagataaa gtgaatttaa gtcttatgga agaagaagat
     3721 aatttttatg gatatttttt gtttcagatg tcaagagaaa taagatttga tataagtagt
     3781 cctacaggcg tgaattttaa gggagctaaa tatgttatat attttaatcc aataatcttt
     3841 ttaacgcttg acataaagca aatggagact acaattaaac atgaaattca tcatatatta
     3901 tctatgcatt tgatgagagc aaaggagtta aaaggaaagt atagtacatt ggcaattaat
     3961 atggcaatgg atatagtagt aaataaattt ctaaacaatt taccaccata tgcaactacc
     4021 ttagagtggg taaatttaaa atattcctta aaacttgaac catatgaacc ttttgaatat
     4081 tatgtagaaa agattcagac tgaattagat ttgatggaag tggatgaaga tggtgaggaa
     4141 gatgatagtg ataaatatgg agatgtagaa attgattata gccccgaaag aactcatgat
     4201 atatgggatg aatccaatga gatagataaa aataccctta gagaatttac tgaaaaattt
     4261 attaacagtt ctcaaaaagg taaagttcct gcttatttag aaagtatgat atcatcactt
     4321 aaaaatagta agggtgaatt gccatggaat ttatatctta atagaataat gggaacagtt
     4381 gaaaataata aaaagaagac tataacaaga agaaatagaa gacaaccaaa tcgtttagat
     4441 ttgaggggag aacttagaag tcataaggca gaaatagcag ttgcacttga tgtaagtgga
     4501 agtattagtg atgaagaatt taaacaagct attaaagaag ttcttagtat agtgaaaaac
     4561 tataatcatg aaattacaat tatagaatgt gataatgaaa ttagacgtgt atataaagtg
     4621 agaactgaaa aagatataaa agatagaatt aggataggtg gaggtactag attttctcca
     4681 gtctttgagt atgctaataa taaaaagatt aatttgttag tctactttac tgatggtaaa
     4741 ggtgaaaata aactacaagt aatacctaga gggtataaaa ttttatgggt tatttctgga
     4801 agaggagata aaatttcatt aaaagaacct tatggagcag ttaaaaaact tactaaggtt
     4861 aaaacaaatg atgaaatatt agaaataaat gatgtaagaa atgatgggta ctcaatgaat
     4921 agccaagcac caattttata ggtattcaca ggagataagc tgtattaatt tattttgata
     4981 tgcttgttat aaaaatctaa aaaatacatt aatcaatggt taatgtattt tttaatttta
     5041 ataatataag ttttacttac atttaagtct taaaatgtgt taaaatataa tatatagcag
     5101 atagttttgg caagatggat ttagttgtca aaactatttt ttttattaag gatggtgatg
     5161 tttttgttta agattgatga ttatataatg tatggtatga caggagtctg caaagtcatg
     5221 gatataacaa atgaacgatt tacaaatgga ataaaaaaag agtattatgt attgagtcct
     5281 atttattcta ataatacagt aataaaaatt cctgtagata atgaaaaagt accaatgaga
     5341 aagttacttt ctaaaattaa tgtgctttca ttaataaatg atattcctaa tatggataca
     5401 tcatggatag ataatgaaaa attaagaagt gagcaattta aaaaaatact cagaggtggt
     5461 aaatgtgaag agttaataaa attagtaaga agtatagata ataacagaga gtatgtaaaa
     5521 tctattggta aaaaaacaca tcaagcagat gataatataa tgaaagaagc agaaaggtta
     5581 ttaagtgaag aatttgcaac tattttagat atttctccta atgaagttag ttcatatatt
     5641 tcaagtcata tacctcaata aagtgtttta gtatccttta tttaatctat tttaagtatt
     5701 atttatgtat gctggttttg gtaaaataaa atatataatt aaattatata aaaaattaag
     5761 aatgattgta ttgaagaatt acagaatagt gcggagagcc cactccttta ggtgtgagat
     5821 ggatatcact taaagattta aattgttatt gtagatgttt ttaatataaa gcaagattaa
     5881 taagtgtatt gtaagcttta agtgaaattg atactataat gagttactta gattttaaaa
     5941 tatataatat tataaaaaat ccttttaggt tttgaatgaa taagatggat actatgttga
     6001 atagaatcca tcttatttaa gttttattta tgttgtttat ttgtaagcat tatttttgta
     6061 catgaaaaca cagtgtaaaa atatgcttag aattatgaag ttttattaaa aatatattcg
     6121 acaaaataag actaaaaata ttcttaaact tataactaag ataaatattg tatatttata
     6181 gtgtagcctg aaaaattgtg gatgtacgaa atttatttag tattttattg aaacaaagta
     6241 gaagaagtac tatagtttat ctttgaatag attgttattt tttagtgaaa aagttattag
     6301 gttaatacat aaatataata gcttataaat agtttaagca tgttaaaaaa gtagttatta
     6361 gagatatata gttacattgc ttataagata cttaaaatga tagcattcac ttatatttag
     6421 aaaattaaga taaaaaatca tttttatata gtaaagcata cctaaattta gaaaataatt
     6481 gaatttataa aattaattaa atacataaaa aagtattcaa ttaacttaat agatttttta
     6541 aaaattaatt taaatttttt cttctattaa ctattaat
//
LOCUS       DAGQWR010000183         6403 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.186, whole genome shotgun sequence.
ACCESSION   DAGQWR010000183 DAGQWR010000000
VERSION     DAGQWR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.186"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     misc_binding    90..301
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            464..1753
                     /locus_tag="MIW95_003496"
     CDS             464..1753
                     /locus_tag="MIW95_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBY3152792.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1807..2886
                     /locus_tag="MIW95_003497"
     CDS             1807..2886
                     /locus_tag="MIW95_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBY3152793.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2907..4451
                     /locus_tag="MIW95_003498"
     CDS             2907..4451
                     /locus_tag="MIW95_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBY3152794.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4614..5156)
                     /locus_tag="MIW95_003499"
     CDS             complement(4614..5156)
                     /locus_tag="MIW95_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBY3152795.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDNKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5304..5588)
                     /locus_tag="MIW95_003500"
     CDS             complement(5304..5588)
                     /locus_tag="MIW95_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBY3152796.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5651..6379)
                     /locus_tag="MIW95_003501"
     CDS             complement(5651..6379)
                     /locus_tag="MIW95_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBY3152797.1"
                     /translation="MGFTSSIPSLIKIKKNPDKFSLKEKFEFMQKQAKKSLDIVNIEL
                     NIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVWRNIPIFKEWAKL
                     LRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPNSLVSEFRSGALK
                     IAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDHIENPRLKSSVLG
                     ENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         2215 a          796 c         1126 g         2266 t
ORIGIN      
        1 taaaaaatgt aagtaattga ctttttaagt tgacaaaata ttgcatatat attatgatat
       61 aaatcataaa ttaactacaa atcaatatta tatattcgta gaagaagaaa gtagcataaa
      121 ggaattttac agagagttag atttggctga gaactaatat tggaatttat gtgaaaagga
      181 ctttggagaa tactattgta attatgttag aatagtggtt taattcgcct taagaaaatg
      241 agtgagtaaa attaattttt actaataaga gtggtaacac ggatttatat tcgtctcttg
      301 gatttttcca agaggcgttt tttattataa acttatttca gaaaatttaa aactatatat
      361 taaatgaaaa ttataaaaat atgatagtta ataaatttat aaattagaaa attgtaaaat
      421 atgctaaggt cagtagtttt acgatttaaa tggagggatg aaaatggaag aagaaaaaat
      481 taactatgct ctggaacaga ttccagagaa tcaaaaacgt ggttgggtag ctatgttttc
      541 agttttagta gcaataggtg tggatttatc atctgttatt ttaggcgctg agttggcaca
      601 gagtatgcca atgaaacaag caatattgtc agtaattgtt ggttcatttt tttctgcaat
      661 tttgtatact acatgctctt tggttggttc atctacaagt ttatctacat ctatgataac
      721 aaagtatgta tttggagaag ctggagcaaa aatattttct ctagttatag gggtttctct
      781 attaggttgg tttggagttc aagtaggttt ctttgcacaa aatgcacaga taataataaa
      841 agatatattt aatttagatg tcagtatgca gattttaagc ctaataggtg gtctcttgat
      901 gatgagtact gcaatttatg gatataaggc catggagaag ttgagtgtgt attcagtacc
      961 atttttatta gttcttatga tgctaactat attcttagct tttagagcaa atggaatttc
     1021 agttgatgat aatatgaaat ctactatgac atttgcaggt ggagtttctc tgtcaatgag
     1081 tataataata gttggtgcaa ttgtatctcc tgatataagt cgttgggcta aaagtagaag
     1141 agactgtgct ctttcaagct ttttgggaat acaatttgga aatgctttta tgataatagt
     1201 ttcgatagta ttagtaaaat gtatgggaac ttcagatatt atgagaattt ttattacatt
     1261 aggaattgct ataccaggta taatagtact tacattagca caatggacta caaatacaag
     1321 taatgtatat tcagcatcat taagtatagc tcttgtactt aagaaggctc ctgagaaagt
     1381 attaactata gtccttggta ttatagcaac attattggct gtatttggaa tatatgaagg
     1441 ttttatagga tttttaaatt tacttggaat tgtaattgca cctgttggtg gtgtatatac
     1501 agcagaatat tacatagtaa agcaagaact taaaggcttt gataaaggag tattatataa
     1561 accaatagtt aaacgttcaa tagtgtcttg gataattggt atattaataa cttacctttc
     1621 aacatatgga tttataactc ttactactat agcaccttta gatggtttta tagcagggtt
     1681 tgtagtacaa tctattattg gaaaagtgct ttgttcaaca aaaaataagc aagaaataga
     1741 caaagcagta taaagaaatt tgtttataaa gtataaaata aattagaaat atgaggagaa
     1801 agtaacatga gatttttaac agaagaaagt gtagataaaa tagcagttgg tgcagcagtg
     1861 cttggaactg gaggtggagg tgacccatat gtaggtaaat tggttgcaaa acaagccatt
     1921 aaaaagtatg gtcctgtaaa agtaataagt ttagatgaac ttgatgatga tgctttggta
     1981 gttccagtat ctggtatggg ttctcctgta attacaatag aaaaattact ttcagaagta
     2041 gagttaacta cccctttaga aataatggag aaattattaa atagaaaagt ggatgttata
     2101 attcctatag aaataggagg gataaactca cttatgccta tagcagttgc tgctaaaaaa
     2161 ggacttccta tcttagatgc agattctatg ggtagagctt tccctgaagc tcaaatggta
     2221 actttttatc ttgaaggata tgaggcatct cctgccgtaa tgtctgatga aaaaggtaat
     2281 tctgccatcc tttatcctgt ggatggaatt tggtcagaaa gattagctag aactttaact
     2341 gtagaaatgg gaggaagttc ttctattagt gactataatt taagtggagc tcaagttaaa
     2401 aaagctgcta taggtaatac tcttacaata gctgaaacta taggagggtt tttacttgaa
     2461 aataaatctg attcaaaagg tgctgttgag aatatactaa aagaattgtg tggatacaag
     2521 ttatttgaag gaaaagtaat tgatattaag agagagttga aaggtggatt tactagagga
     2581 catgcctttt ttgctgggat taatgagtat gatggagagt attctatatt attccaaaat
     2641 gagaatttaa ttgccaaaaa aggtgataca ccactttgta ttacaccaga tttaatagca
     2701 gtattagatt tagaaactgg atttccaata actacagaga gaataaaata tggctctcgt
     2761 gttatggtag tagcttttcc ttgtaatgaa aagtggagaa cagaaaaagg aattgagaca
     2821 gttggacctg gatactttgg atatgatgtg gaatataaaa cagtagaaga attacaagga
     2881 aaataaaagt ttaggaggaa attttgatgt atcgtgtagg aattgacgtt ggaggaacaa
     2941 atacagatgc ttgtatactg gatggggaat taaaagtaat tcattcagtt aaggtggcaa
     3001 ctacaaaaga tgttgaaact ggtgtttaca atgccttaaa aaaagttata gatgaaagta
     3061 agattgacca ttcattaata aaatatgcta tgcttggtac aactcattgt acaaatgcta
     3121 ttgtagaaag aaaaagactt aataatgtag ctatgataag aataggaaag ccggccacta
     3181 ctgccattct tccatttata gattggccag aagacttaag agaaaaaata gaattagatt
     3241 caatattggt atctggtgga tatgagtttg atggtagaaa gataaatgaa ctttgtaaag
     3301 atgaagttgt agagttttgc aatagaataa aaggcaaagt ggaaagtgtt gcaataagtg
     3361 gagtatttgc tccagtaaat aaacaacaag aagaagaagt tgcaaaatgg gttagagaaa
     3421 ttttagggga tattcctata tctttatcaa atgaaatagg taatataggt atattagaaa
     3481 gagaaaatgg agctatttta aattctgcat tgtctcaagt aggtaaagct gtatctttag
     3541 gatttgaaaa agcacttaaa gacttaggta tagatgctca aatatatttc agtcaaaatg
     3601 atggaacatt aatgaattta gagtatacaa tgaaataccc aatttttaca attggatgtg
     3661 gaataacaaa ttctataaga ggagcttcct tcttatcaaa agtaaaaaat gcaatagtat
     3721 tagatgttgg tggaactaca agcgacttgg gtgtactata taatggattc cctagagaat
     3781 catctattcc agttacaatt ggaggagtac atacaaactt tagaatgcca gatgttttaa
     3841 ctattggtct tgctggtggt acatgtgtaa aaggcgaaaa agataatata aagataggtc
     3901 ctgaaagtgt gggatataga ataactgaag atgcaataat atttggtgga gacactctaa
     3961 ctttatcaga tgttgttaca aaattagata tggcgtttga agatagaaaa agcaatgttg
     4021 aaaacttaga taaagagtat tgtaatgagg tatataagaa ggttatttta aaattagaag
     4081 atgctgtaga ccaaatgaag actagtcaag atgatgttga attggtttta actggtggag
     4141 gaagtataat tgttcctgaa aaactaaaag gcataagcaa tgttataaga cctcctcatt
     4201 atactgctgc aaatgctatt ggtgcagctt taggacaaat aagtggagat gtagaaaaag
     4261 tatacagttt agataaaatg tcgagagaag atgccataga agatgcaaaa aaagaggctg
     4321 taaataaagc tctaaaagct ggtgctaaag aagatactat agaagtatta aatatagaag
     4381 atgttccact tgcatacctt cctggtaatg cacttcttat aaaagtaaaa gttgttggtg
     4441 atttaattta aagaataaat ataagtattt ccagaattat ataaatattt ctggactcat
     4501 ttttaaactt attttatcaa taaaaacagc ataacttttg actatattct tataagtttc
     4561 cgaacagccg atatatacct attttttgag gtttatcggc tgtttgttat cttttatcgc
     4621 attccaacta tttttcttac cgtatttgct gtacgaattg taatcttatc acttacattt
     4681 gaactggcag tctttgacca ccaatttgtc tttttataat ctttacgact aaaagaccaa
     4741 aagatagcat ttttataatg atatactttt tcatactctt ctttaggatt tccaacctca
     4801 tcataaaact cgtcataaga taagtcagga aacataaaaa taagattatc atatatttct
     4861 ttgttgttat ctccccacca attaggagca ttatttagta tatcttttaa ctcctcttgt
     4921 gaaataataa aaactggtat ttccaattca aactgttttt ttatcattaa tcttattgta
     4981 tttgagataa ccttgttgtc atctacatca cttaaaaaga caacattacc actattaagg
     5041 tatgtagaaa cttctgaata accaagttct acaaagcttg cttttaattc agacataatt
     5101 atcttgttct taccacttat attgatacct cttaataatg cgatataact tttcatataa
     5161 taagttcacc tctaaacatt cacatttttc tatgtagtac aaactggaat ttgcatcttt
     5221 tataattctt tcaaaacttc ttcatatgta atttttttaa tataaaaaaa tcggtgctta
     5281 atttgtaccg atttttattt ttcttagtaa agaactcgtt tttttgcttc tgctatagct
     5341 ctagcagcac tttttgcttg cattcttttt tcttcttctt ttttaaattc atttattgtt
     5401 tcatttagat tcttatcatg ttcatttttt tctttatctt cgttttctct gtatactaat
     5461 atataatcta ttattgattc gtttattatc tgagatatac ttttattttc attaattcct
     5521 attctcttaa cttccttaag tagagtctca tcaatatgaa ccgttgtttg ctttgtattt
     5581 tttgccacct taatcacctc ttaaaaaaaa ttctaacatt aaaatttttt ttagtcaata
     5641 aattttaata ttaaaattct ttaaatgttt tatttgattt tctaaaattt tctattgtat
     5701 ttatcatctt attttttata ttttcaccta gtacagaaga ctttagtcta ggattttcta
     5761 tatggtcata aattggttct aaaaattcga cttcgacagg aacagagtta attttaccaa
     5821 taggttggta tccttcataa gtatcttttg aattttttat tacgagtgga acaatagggc
     5881 attttgcttt atatgctatc tttaaagctc cacttctaaa ctccgaaacc aatgaatttg
     5941 gttctttaat ccatgttaaa tctccttctg gaaacactgc catactttga cctgtaagta
     6001 tattttgaga ggcttgggct atactcttta taccctcacg attgtttttt ctgtttacat
     6061 atacacatct tagtaatttt gcccattctt taaatatagg tatatttctc caaactggtt
     6121 catctgcaat tacacatcca attggtcttt ctacacttgc tgttagtatg aagctgtcaa
     6181 gcatactaga atgatttaca acaaatagaa gtggttcttt tggcagtgtt tccttaccaa
     6241 ttatattaag ctctatgttc actatatcta aagatttttt agcttgtttt tgcataaatt
     6301 caaatttttc ctttaagcta aatttatcag gattcttttt aattttaata aggctaggta
     6361 tagaagatgt aaaacctata agttgatatg tagcaaattt taa
//
LOCUS       DAGQWR010000184         6384 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.187, whole genome shotgun sequence.
ACCESSION   DAGQWR010000184 DAGQWR010000000
VERSION     DAGQWR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.187"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            122..889
                     /locus_tag="MIW95_003502"
     CDS             122..889
                     /locus_tag="MIW95_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HBY3152798.1"
                     /translation="MGLFGKKKADICCICNTEIGVLNIEDGWICNSCFKEYCDALSMT
                     KAPKILRKLDIEKTISSTKKNNELQKIFNTTNNIENYIEFDEDNKKWLVSKKSINDKK
                     TPIIHSYEDIVEFELLENGETVTKGGIGRALAGGILFGEAGAIVGGITGKKTTRKVVD
                     TFKIKITINNIDNPIEYIELINKKTKTNSSAYEKAYKDAHKILSTLSAITQSIKETDN
                     INTKSVADEILKYKNLLDMEAITQDEFNTKKKELLNL"
     gene            955..3327
                     /locus_tag="MIW95_003503"
     CDS             955..3327
                     /locus_tag="MIW95_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBY3152799.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTTSINTTINSLERLQQRLHNPLN
                     TGGIQASQQSLNNIENILTRIEQKIGRNTNEQENFNNKIKQGSEAGSLLVSKLKSLAG
                     IYIGIRGIESITKAADTIASTKARLNLMNDGLQTTEQLNKMIYLSAQSARASYADTAA
                     QVAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPVTFSQALTMMKNDAYMIFGQTLGKISGALQSVRFSEIVVSIRNVMIAISSNIYDT
                     LNIIKNILNSEFFSNLVQGFTFCAVVITKSLGSIVNTALNIVNIFAQNWSIIKPIVFG
                     VASVFILFRGVLLATKIATIGNVIANVAHAASSSLSALMTNIQAAALMRSNGATLSAT
                     IATWGLNAALLACPITWVVLGILAFVVVVFVAVAAVNKFAGTSLTALGVIVGAVFAAV
                     AAIQNVMIWLLNGCIAVNEGITNGWNQCVYLMKQAIAKGVIFIIEKMASLNNSVNNAG
                     NALGKAFIDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYIKQQIDSL
                     NRWVGDAPEKVKLERMGYKDIGAAFQKGNALGTKWQNAITDKFKDTFDINKMLEDAKK
                     KLGLDDLWNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQ
                     ETINRFTGVNIKIDMNNTNNISKDTDVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            3344..4033
                     /locus_tag="MIW95_003504"
     CDS             3344..4033
                     /locus_tag="MIW95_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBY3152800.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKNLSKY
                     TIYKGQVLKLG"
     gene            4026..5918
                     /locus_tag="MIW95_003505"
     CDS             4026..5918
                     /locus_tag="MIW95_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBY3152801.1"
                     /translation="MVDELVLANDRDVRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKILAKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLRE
                     KKEMYTLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSAHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSIMKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLNKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSNTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            5932..6192
                     /locus_tag="MIW95_003506"
     CDS             5932..6192
                     /locus_tag="MIW95_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBY3152802.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKMGDKLVLIRMQGGQQYLILDRM"
     gene            6197..>6384
                     /locus_tag="MIW95_003507"
     CDS             6197..>6384
                     /locus_tag="MIW95_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBY3152803.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYE"
BASE COUNT         2575 a          752 c         1224 g         1833 t
ORIGIN      
        1 tatgaagcat ttactttaaa taagtaagtg cttttttaat taaaaggagg tgataataat
       61 gtaaaaattt tacttatata gtataattac attataaaat tattgtactg gggggaatct
      121 tatggggtta tttggaaaga aaaaagcaga tatatgttgt atttgtaata ctgagatagg
      181 agtactgaat attgaagatg gttggatatg caattcttgt tttaaagaat attgtgatgc
      241 actttctatg actaaagcac ctaaaatttt aagaaaatta gatattgaaa agactatatc
      301 atcaactaaa aaaaataatg aacttcaaaa aatatttaat acaactaata atatagaaaa
      361 ctatatagaa tttgatgaag ataataaaaa atggcttgtg tctaaaaaaa gtataaacga
      421 taaaaaaact cctatcattc attcttatga agatatcgtg gaatttgaac ttcttgaaaa
      481 tggtgaaact gtaactaaag gaggaatagg aagagcttta gcgggaggaa ttttatttgg
      541 agaagcagga gctatagttg gagggataac aggtaaaaaa acaacaagaa aagttgttga
      601 tacatttaaa attaaaataa caattaataa tattgataat cctattgagt atatcgaatt
      661 aattaataaa aaaacaaaaa ctaattctag tgcttatgag aaagcttaca aagatgccca
      721 taaaatttta tcaacattat ctgctattac acaaagcatt aaagagacag acaatataaa
      781 tactaaatct gtagcagatg agatattaaa atataagaat cttttggata tggaagctat
      841 tacacaagat gaatttaata ctaagaaaaa agaattattg aatttgtaat ataataaaca
      901 cttactaatg taggtgtttt tttatatgga aatttatgaa aggagagtga gaaagtggca
      961 acgatacaaa cttcaataaa gattttcgac ggaatgacac ctgcttttag gaatatgact
     1021 acatctatta atacgacaat taatagcctt gaaaggttac aacaaagatt gcataatccg
     1081 cttaacacag gtgggataca agcatctcaa caaagtttaa acaatattga aaacatatta
     1141 accaggatag aacagaaaat tggaagaaat acaaatgaac aagaaaactt taataataag
     1201 ataaagcaag gaagtgaagc aggttctcta ttagtatcta aactcaaaag tttggctggc
     1261 atatatattg gaataagagg tatagaaagt attacaaaag cagcagatac aattgcaagt
     1321 acaaaagcac gtttaaattt aatgaatgat ggattacaga caacagaaca acttaataag
     1381 atgatttatt tatctgctca aagtgcaaga gctagttatg cagatacggc agcacaagtc
     1441 gctaaacttg gaatattagc aggagatgct tttggaagtt cggcagaggt gataaagttt
     1501 acagaactta tgaataaagc ttttgtaatt ggaggaacat cagcaaatga agctagtgcg
     1561 gctatgtatc agcttactca agctatgggt gcagggaaac ttcaaggtga tgaatttcgc
     1621 tctataatgg aaaatgcacc tttattagcc actaaaatag ctgatgcaat gggaaaaact
     1681 aaagaccaat taaaggaatt gtcaagtagt ggagcaataa cagcagatgt tataagaaat
     1741 gcactattta aagcttctga tgagatagaa aagaaattcg caagtatgcc agtcactttt
     1801 agtcaagcac ttactatgat gaaaaatgac gcctacatga tttttgggca aaccctcgga
     1861 aagataagtg gagcattgca aagcgttaga tttagtgaaa ttgttgtatc tataagaaat
     1921 gttatgattg caatatcttc aaacatttac gatacattaa atattataaa aaatatttta
     1981 aatagtgaat tcttttcaaa cttagtgcaa ggatttacat tttgtgcggt agtaataact
     2041 aaaagcttag gttctattgt aaatactgca ttaaatatag ttaatatatt tgcacaaaat
     2101 tggagtataa taaaacctat tgtatttggt gtagcttctg tttttatatt gtttagaggg
     2161 gttttgctag cgactaaaat agctacaata ggtaacgtaa ttgcgaatgt tgctcacgct
     2221 gcttcctctt cactatcagc acttatgact aatatacaag cagcagcact gatgcgttcg
     2281 aatggagcta ctttatcagc aacaatagcc acttggggtt taaatgcagc tttattagct
     2341 tgtccaatca catgggtagt attaggtatt ctagcatttg tagtagtagt ttttgttgca
     2401 gtagcagcag taaacaaatt cgcaggtaca agtttaactg ccttaggagt aattgtgggt
     2461 gctgtatttg cagcagtggc agcaatacaa aatgttatga tttggttatt aaatggctgt
     2521 atagctgtaa atgaaggcat tacaaatggt tggaatcaat gtgtatatct aatgaaacaa
     2581 gctattgcaa aaggtgtaat ctttataatc gagaaaatgg catctctaaa taattctgta
     2641 aataatgctg gaaatgcact tgggaaagct ttcatagatg gggcaaatat cgcaatacga
     2701 ggtgtaaaca aattaattga cctaataaat aaaataccag ggataaatat tggtaaagta
     2761 ggagaggcaa cgtttacgcc agttaaggca gataatagtt acatcaaaca acagattgat
     2821 agcttaaaca gatgggtagg agacgcacca gagaaagtaa aattggagcg aatgggatac
     2881 aaagatattg gagcagcatt tcaaaaagga aatgcacttg gaactaaatg gcaaaatgct
     2941 ataactgata aatttaaaga tacttttgac attaataaga tgctagaaga tgcaaagaaa
     3001 aaattaggat tagacgattt gtggaataaa caaaatcctt taaacaacct tggtggattt
     3061 ggtggagatt taggaaaaaa tgtaaaggac acggcaggca atactgcaaa gatggcaaag
     3121 actatggata aaagtcaaga ggacttaaaa tacttaagag atatagcaga gcaagaaaca
     3181 atcaatcgat tcacaggagt aaacattaaa atagatatga acaatacaaa caacataagt
     3241 aaagatacag atgttgatgg aatagttaat gtcttaactg aaaaactgaa cgatgctatg
     3301 gttgtatcag cggaaggaat agtttaggaa ggagagtgag aaaatggctt atgattttta
     3361 cctagatgga gtacaactac caatcgcacc acccaagctt gaagtcaaag tgacaaacaa
     3421 gaacaagaca gttgatttga taaatactgg agaagtaaac atattaaaaa aagaaggatt
     3481 atctgaaata agttttgaag cagaatttac acataataaa ctaccttttt gtagaggtca
     3541 atttagagat gttcaattct ttttaagtaa gctagaatta ctaaaaactg attgtaagcc
     3601 atttcaattt attgtatctc gtgagttagg taacaaggtc ttatttaaca ctaatataaa
     3661 agtatctctt gaagagtatg ctatttcaga agatgcagat aatggctcag atacaaaagt
     3721 tgcaataaaa ttaaagcaat atagagatta ctcaactaaa aagttagttc ttgcccctcc
     3781 taaaaatgag actggtagac ctaatgtaaa gatagagcca aaacgagttg attcagtcaa
     3841 tgccacaaac actaaaacat atacagtaaa agcaggggat agcctttggt caatttgtca
     3901 gaaacaactt ggtaatggtt cattatataa gaaagtatac gaactaaata aatctatgat
     3961 ggataaggca aataagggca aaaacttaag taaatacact atttataaag ggcaggtgtt
     4021 aaaacttggt tgatgaatta gtgttagcaa atgatagaga tgtaagatta gtaatagctc
     4081 attgggaaga tttctacgaa cctgctgtca ttgatggtat cacatgggag atagaaagac
     4141 gaggtacacc ttctaagtta gagtttacaa tagtcatgga tgatatacta gagttttgcg
     4201 aaggaaattc tgtaaggctg tattataaag gaataggcat attttatgga tatatatttc
     4261 aaaagaaaag agataaagaa aatcacatta aaattgttgc ttacgaccag ttgagatatt
     4321 ttaagaataa agatacttat gtatatagta ataaaactgc aagtgaactt gtaaaaatct
     4381 tggctaaaga ttttaattta aaatacaatg tcatagaaga tactaagtat aaaatatcta
     4441 gagtcgaaga aaataaaaca ctctttgaca tgatactaac agcactagat gatactttaa
     4501 gagagaaaaa agaaatgtat accttgtatg atgattttgg aagaataaca ttaaagaatg
     4561 ttgcatcaat gaaactagat actgtcatga acaatgatgt aattgaggat tttgactata
     4621 attcatcaat agatagtgat acttacacaa agattaaact tgtaagagac aacgaggagt
     4681 caggaaaaag ggatgtatat attgctcaag actcagctca catgagaagt tggggaatac
     4741 ttcaaatgtt tgatacagta gacaaaaaca tgagtgaagc agagataaaa caaaagtgtg
     4801 atatacttct aaaactatat aataagaaaa ctaagtcatt aagtttaaaa aatgcacttg
     4861 gagatattag agtgagagca ggttgtttag tacctgtttt tttaaatcta ggagatattg
     4921 aattgcaaaa ttatatgtta gttgagaaag taaaacatac atttgaaaat aattcacatt
     4981 tcatggattt gaccttggtt gatggagacg aatttgcttc atattcttca agctcatata
     5041 gtagtggaaa tactaataat aaagatgaaa agaaaaatgg tcctgcacaa agtattatga
     5101 aaaaaaatac aggtaaaaaa gttcctgcta tatttactgc atattatcca ggaaacaatg
     5161 caatggaagg tggaaaaaca gattgcaatg gaaagccact tgatgtaaaa tcaagaactg
     5221 ttgctggtcc aatgaatcga gaaggagtta agaaaacttg gtatactgat gattttctaa
     5281 ataaacatcc agtttttgaa tatggagata aagtaaaaat tatacttcct ggtactgcct
     5341 atgacaacaa agtatataca gttaaagata atggaggaag aatatatgtt gaaacaaacg
     5401 gaacatatca tatagatata ctattagcta atgctagtga atgtaaaaaa tttggtagaa
     5461 agaatggcta tataattata ggtggagatg aagaacaaac atatcaagtt gaaggtaata
     5521 accaaagtaa tacaaataac aattctaaag aagataaatt aattagtata gcaaaaagta
     5581 aactgggttg taattatgtg tatggagcag aaggtcctaa taattttgat tgcagtgggt
     5641 ttactcaatg gtgttataaa caaataggta taaaaattcc tcgtactgct tctgcacaaa
     5701 gtaaagcagg aaaagcagta gatttaaaag atagaagcaa gtggaaagca ggagacttat
     5761 tatgtagaat tggtggagga agtagtaatc atgttgtaat gtacattgga aacaatcaaa
     5821 taattcattc accacaaaca ggagatgtgg taaaaataga gtctgttaat tcttatagaa
     5881 aaggaaaagc atacacacat gtgagaagat ttatataagt gaggtggcaa tatgagccaa
     5941 gatttattac agataataaa aaaagctgca atggatgcag tagaaacaag caacccaatg
     6001 agggttgtat ttggaacaat agaaagtatt agtcctctaa gagttaagat agaacaaaaa
     6061 ctatctattg gtgaaatttt tctaatacaa acagatacat ttaaaagata tacagataaa
     6121 aaaatgggag ataaattagt cttaattcgt atgcaaggag ggcaacaata cttgatttta
     6181 gataggatgt gataaagtgt taccaagtga taatttagat tatgatattg aagatgtatc
     6241 aataattaat tttgatgtaa gacaagagcc aagtaagaca tttaaacttc atatagaaaa
     6301 gtctaagatt gatggtattt gtgatgatgt agaagcatta aaacaaacca tttttttaat
     6361 tttaaatact gaaaggtatg agca
//
LOCUS       DAGQWR010000185         6304 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.188, whole genome shotgun sequence.
ACCESSION   DAGQWR010000185 DAGQWR010000000
VERSION     DAGQWR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.188"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(204..2798)
                     /gene="clpB"
                     /locus_tag="MIW95_003508"
     CDS             complement(204..2798)
                     /gene="clpB"
                     /locus_tag="MIW95_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBY3152804.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSDDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(2835..3431)
                     /locus_tag="MIW95_003509"
     CDS             complement(2835..3431)
                     /locus_tag="MIW95_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152805.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(3448..3852)
                     /locus_tag="MIW95_003510"
     CDS             complement(3448..3852)
                     /locus_tag="MIW95_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBY3152806.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNICFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            4219..4593
                     /locus_tag="MIW95_003511"
     CDS             4219..4593
                     /locus_tag="MIW95_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY3152807.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            4590..5453
                     /locus_tag="MIW95_003512"
     CDS             4590..5453
                     /locus_tag="MIW95_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152808.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRNPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            5453..6091
                     /locus_tag="MIW95_003513"
     CDS             5453..6091
                     /locus_tag="MIW95_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBY3152809.1"
                     /translation="MKGLILKDLLNLKGNVKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT         2090 a          942 c          719 g         2553 t
ORIGIN      
        1 acagtagttt tttataatta tattcaaatt atgattatat tataagaaac ttactaaaat
       61 acttcctttg agttaaattt aatttggaca tattgactta aaaatgtttt attaatttga
      121 taaaatattt cttttcctat agtttataaa taaaaatcag ctgagcaaaa tatttagtat
      181 attatgctca actgattctt ttattattta actaaaactt caatattttc atcttttcca
      241 tctataacta tagtacatcc attatacaca tctccagcaa taagtttctt tgcaattata
      301 gtttctaatg tatttcctat atatctctta agaggtcttg ctccatatac agggtcatat
      361 ccttctctca ccataacatc ttttgctgaa tttgtaactt caatagtaat atctttttct
      421 tttaatctat tttttaaatc tttcataaat atatctataa ttttcttgat attttcttgg
      481 tctaaaggct taaacattat aatatcatct actctgttta aaaactcagg tttaaatcta
      541 tgcttcattt cattcattac taaattgtta gtagtctctg ttatatttcc tcctgcttct
      601 aatagataat tacttcctat attagaagtc attattataa gagtattttt aaagtctact
      661 gtcttacctt tattatcagt aagtctacca tcatctaata tctgtaagaa catgttaaat
      721 acatcctcat gtgctttttc tatctcatca aatagtatta cagaataagg agcacgtcta
      781 actgcttctg taagttgacc accctcttca tatcccacgt accctggagg aggtccaact
      841 agtcttgata cagcatgttt ttccatgtat tcagacatat ctattcttat tatattatcg
      901 tcactatcga ataaatttct agcaagagtt tttgcaagtt cagttttacc aacacctgta
      961 gggcctaaga aaataaatga accaattggt tttctttcat cttttaatcc tgctcttgca
     1021 cgtattactg catttgaaac tgctgtaact gcttcatctt gtccaataac tcttttatga
     1081 agttcatctt ctagtttaag taatttttct ctttctcctt caactagttt agttactggt
     1141 atacctgtcc actttgatac aatgctagat atttcttctt cagtaacttc ttcttttaaa
     1201 agagaatttt cagaaccatc attcatattt tcttcgtact gctttatttg ttcttctaac
     1261 ttaggtattt caccatattt aacctctgct gctttgttga aatcatattc tctttcatac
     1321 ttttcagctt tacctttagc ttcgtctaat tgagccttta agtttttaat atctaatatt
     1381 tgactctttt ctttttcata ttttgctgtc atttcatcat ttttagattt taactctgct
     1441 atttcttttt gaatatcatc caatcttgat ttgcttttat catcattttc tttaagtaat
     1501 gcttctcttt cagtttctaa tgtaaataac tttcttctaa caacatctaa ttctgttggc
     1561 aatgaatcta tttcactacg aatcatagct ccagcttcat caattaaatc aattgcttta
     1621 tctggtaaaa atctatcttg tatatatcta tgagaaagtt ttgctgctgc tacaatagca
     1681 ttatcatgta ttcttactcc atgatgtatc tcaaatcttt cttttaaacc tctaagtata
     1741 gatattgtgt cttcaacagt aggttcttct gcaattacag gctgaaaacg tctttcaaga
     1801 gccttatctt tttctatata ctttctatat tcatcaaaag tagttgcacc aatacagttc
     1861 aattcacctc ttgcaagcat aggtttaata agattacctg catccattga accttctgtt
     1921 ttaccagctc caacgatagt atgaatctca tctataaaca gtataatctt tccatcagaa
     1981 ctttgaactt cttttaaaac tgcctttaat ctttcttcaa attcacctct atattttgca
     2041 ccagcaatta aagctcccat atcaagtgag aatacaatct tatcttttaa accttctggg
     2101 acatctcctc ttactattct ctctgctaac ccttctacaa ttgcagtttt acctacacct
     2161 ggctcaccaa tcaatacagg attatttttt gttcttcttg ataaaattct tataatcctt
     2221 cttatttctt catctctccc aattacaggg tctaatttat ttttcttggc taaatctact
     2281 aagtttgttc catatctagc aagtgcatca tatgtgcctt ctgggtcttg tgtttctact
     2341 ctttgactac ctctcacttg tgataaaata tttaaaaagt cattcttatt tatattatat
     2401 tgttttaata ttttaccaac agctgatttt gattctgttt ccatcattgc aagcataaca
     2461 tgctctacac ttatatatga atctttaaat tctttagaaa tactctctgc ctttataagc
     2521 acctcattta tttttctagt agctgatact ccttgtgagt cagcaccttc tccatatact
     2581 tttggaagct tatcaatttc aaagtttaca gtatttctca aagaatcaat tgatatattc
     2641 attttttcga ttatatttgg tatcaatcca tcctcttgat taataagtgc tgagaaaaga
     2701 tgaattacat ctacttgttg gttatgattt tttactgctt cgttataagc ctcatttaaa
     2761 cttttttgaa ctcttaaagt cattttttct acgtccatat aaatccctcc attttaatat
     2821 tatatccaaa taaattaaat tctctcattt ataattattg attttctaag ctctaaaaga
     2881 ctatcaagta aattctcgtt tttttgtata tcgaaactca atgttaaatt aaatgttcca
     2941 tctgaattaa aaaaatcgaa tacttctttt gattttgcta ttaaatacaa gtcttcatct
     3001 ataagtcttt tttcacctat taataaatac tgacatttat ctataaaact ctttttaaaa
     3061 tcaatagtgt atagattttc taaaatatta tattgataaa tagcaatatt attaaacact
     3121 tttaatatgt ttgaatttac aaaattgtaa atttgattat tgttatacag tgatatttgt
     3181 tcgtatatat attccctaaa taaaggatga atgttttgct taagttttaa agtaacttca
     3241 gctggtattt caaaataaag cttccagtac ttagagttat ttttttctaa aataaaagct
     3301 actttgttca aactggtaga actaatctcc tttagtgtac aattaaaatt atagtgtctc
     3361 aaactagttt tttcattctc aaatacatag tcaaaccact gtagaacatt tctatcaagt
     3421 tttaaactca tataaaaacc ttcttcctta tttactaacc ttattaggtc tttttatctg
     3481 attatattct gctaataaac tttctttatc agctctacta cctttatatg ctatatgtgg
     3541 atttatcata tactcaccat ttttcactct ttgtaaaaaa tccatttttt tcaactccgt
     3601 aaacaatgaa gataaagaag tttttggata ttcaaattca caagataatt catataatgt
     3661 ttttctaatc ttattatttt tatccataga gtttattaat tttaatggaa ttaaatccct
     3721 cttagtatac attccttcta ctactttaat ataattctta aaacaaatat tcatatactt
     3781 acttttattc ataactaaac ttttgctctc tgtatattcc tgatttaaat ccttttttaa
     3841 tgtcttatcc aaattaaata tctcttctca ctctccttat ctctaattga taatataaat
     3901 atattacaaa aaaatatgta tgtcaaaact gaaaatgcaa aaagtttacc ttataagtaa
     3961 acttttgttt agctataagc taaactttat tattttttta atttagtatt tccaatttat
     4021 tggcaggatt tttgaataat atttataaat tttttaaaaa aatttaaatc tatttaatat
     4081 ggcagccttt cataaaatac attacattta ttaaatgtat aatattggta attagttttt
     4141 tctaaaatca gttgacatat tatatatata tttatataat gtgtatatac tatatataca
     4201 atttaaggag gtgacttatt gaatatagtt attagtaatt cgagtgggaa gccaatatat
     4261 gaacagatta ctacacaaat taaaagtatg attataagcg gagagttacc agaaggctca
     4321 gctcttccaa gtatgagact attggcaaag gaacttagaa taagtgtaat tactacaaaa
     4381 cgagcatata gcgatttaga acgagacgga tttattgaga cagttactgg taaaggcagt
     4441 tttgtatcta gtaaaaacat ggattttgtc aaagaagaac aatttcgttt aattgaagaa
     4501 tacctacaaa aagcagttga tacagcaaaa agtagtgata ttacatatga cgaattaaaa
     4561 gatattttat ttttattata taaaggagaa tgataagtat ggatgcaata actataaaaa
     4621 atttaaataa aacttataaa gattttagcc tacaagatat atctttttca gtaccaaaag
     4681 ggagtgtaat gggatttgta ggtgaaaatg gtgcaggaaa aacaactaca ttaaaagcaa
     4741 ttttaaattt aatatcttat gatagtggaa atatagaaat atttggtctt gatagtaaaa
     4801 aaaatgaaaa agaaattaaa gagcaaattg gggttgtatt tgagggaagc aactttcatg
     4861 aaaatttaaa cactgaccat gtttcaaaaa tcatgagtaa aatctataaa aattggaatg
     4921 acacattatt taaagactac ttaaaaaaat tgagagtacc agacaataaa ttaattaagg
     4981 agttttcaaa ggggaataag atgaaactta gtattgctgt agccttatct cataaaccaa
     5041 aattattaat actagatgag gcaactagtt cacttgaccc tatcgttcgt gaagaaatac
     5101 tagatatatt tttagatttt atacaagatg aagaacactc tattatctta tcttcacata
     5161 ttacaagtga tttagataag atagcagact atattacatt tatacataaa ggaaaaattg
     5221 tatttagtga aaataaggat gaattaattg atactatggg agttttaaaa tgtaaaccta
     5281 gtgattttga taacctttca agagaggatt attcatatta tcgtaaaagc caatttggat
     5341 atgaagtact tcttaaagat aaacatagat ttatatcaag aaatccaaat tgtattgttg
     5401 ataatacgtc tatagaagaa attatgttat tttatgtgag aggtgataaa taatgaaagg
     5461 tttaatttta aaagatttac ttaatttgaa aggaaatgta aagtttatac ttttattcat
     5521 cattatgttt gggtttatgt cttctttagg agatggaaat gttaataact ttataggtgt
     5581 tattatagtt ttatgtacta caatgattgt ttcaacattt tcatatgatg atttaaataa
     5641 atgggatagt tatgtactta caatgcctat taatcgcaat gatattgtat taagtaaata
     5701 tttaactatg ttaatattta gctttatagg tgttcttgtt tcacttattg ttagtgtaac
     5761 tattggttat tttaaaaata cacttatatt aaatgaaaca ctacttatta atgcactaat
     5821 cttatcaatt tcagtatgtt ttggtagcct tattctacca cttatttata aatttggtac
     5881 agaaagagct cgtttattaa tgattttatg ctttttagtt ccaacactag ctttattagt
     5941 ttttaaaagt attttagaaa atattagttc acctatttct atagaaatta tacttaatac
     6001 actagtatat tctcttccat ttgttgctat tttactattt gtgatttcat attttatatc
     6061 atcaaaaatc tatagtaaaa aagaagtata aaatatacta tacatgtctc tgtatttaag
     6121 tattttaaat aaaatactat caagtttatt ttggtagtat tttatttttt tattattaat
     6181 atctactagg tatttattgt tttattactt atatttaata agactattta ctactttcta
     6241 atcttattta tataatttat tcattttata aattgaacta ctaatgatat tattgaacat
     6301 agca
//
LOCUS       DAGQWR010000186         6258 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.189, whole genome shotgun sequence.
ACCESSION   DAGQWR010000186 DAGQWR010000000
VERSION     DAGQWR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.189"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            170..313
                     /locus_tag="MIW95_003514"
     CDS             170..313
                     /locus_tag="MIW95_003514"
                     /inference="COORDINATES: protein motif:HMM:NF014609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBY3152810.1"
                     /translation="MNKKFRLYQVDSFTKERFTGNPAGVITNADGLSESQMQKITREL
                     NNS"
     gene            335..1090
                     /locus_tag="MIW95_003515"
     CDS             335..1090
                     /locus_tag="MIW95_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HBY3152811.1"
                     /translation="MAKNHQENYDVHIRFFTPTNEVPICGHATIAAHYVRAVENQFNN
                     KTVFQKTGAGILPVDIIKKDNDYEIIMTQGKIEVSPPLQQTYQKRLLNAFGILNEDIR
                     TDCPIAISSTGHSKVMIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHNKETL
                     QVHGRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPIPNTEFIFEAVQGEAIKRKGK
                     MLVKVIISDNKPVEVKIIGKAVIAFSTNIEIEL"
     gene            1315..1926
                     /locus_tag="MIW95_003516"
     CDS             1315..1926
                     /locus_tag="MIW95_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBY3152812.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIIEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            2224..2562
                     /locus_tag="MIW95_003517"
     CDS             2224..2562
                     /locus_tag="MIW95_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBY3152813.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(2969..4405)
                     /gene="miaB"
                     /locus_tag="MIW95_003518"
     CDS             complement(2969..4405)
                     /gene="miaB"
                     /locus_tag="MIW95_003518"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBY3152814.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(4653..6089)
                     /locus_tag="MIW95_003519"
     CDS             complement(4653..6089)
                     /locus_tag="MIW95_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBY3152815.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPIVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT         2215 a          912 c          694 g         2437 t
ORIGIN      
        1 tagcatagag ccttacataa tactactttt ttagatgtat atttttaaat tttaatatag
       61 acatatgtta ttttattaat tttatttaaa cagataataa taaataataa tttatctcaa
      121 gttttctatg tagataaact aatctaatgt ttagaaagga ttatataata tgaataaaaa
      181 atttcgtctt tatcaagttg actcatttac aaaagaaaga ttcacaggta atccagctgg
      241 tgtaattaca aatgctgatg gcttatctga atcacaaatg caaaaaataa caagagaatt
      301 aaataattca taaactgctt ttattttcaa taaattggct aaaaatcatc aagaaaatta
      361 tgatgtacat atcagatttt ttacaccaac taatgaagtt cctatatgtg gtcatgctac
      421 tattgctgcc cattatgtta gagcagtaga aaaccaattc aataacaaaa cagtatttca
      481 aaaaactggt gctggaatat taccagttga tattataaaa aaagataatg attatgaaat
      541 aattatgaca caaggaaaaa ttgaagtatc tccaccatta caacaaacct atcaaaaaag
      601 attacttaat gcatttggta ttttaaatga agatattcgt acagattgtc ctattgccat
      661 atcatctaca ggacattcaa aagttatgat tggaataaag gaattatcaa cattaaatag
      721 tttatctcca aatttatatg aattatcgaa aataagtcaa gaaataaaat gtaatggata
      781 ttatattttt acattacata ataaagaaac tctacaagtt catggaagaa tgtttgctcc
      841 tgcaattggt atttcagaag accctgttac aggaaatgct aatggacctt taggtgcgta
      901 ccttgtacat tataaagtaa tacctatacc caacacagag tttatatttg aagcagttca
      961 aggagaagca ataaaacgca aaggcaagat gttagtaaaa gtaattattt cagataataa
     1021 gccagttgaa gtaaaaataa ttggaaaagc tgtaattgca ttctcaacaa acatagaaat
     1081 cgaattatag attctttcta agtaaatttt tgaacttttt aatactaatt taataacaaa
     1141 ctaatttata tacaatcaaa ttaatttttt tattatttgt taatatatat ttatcattat
     1201 attatataat aatatagata gttactatat cttataatta gataaatcat aattatatat
     1261 tattgttaaa actcaattat ataattttta ataatattat tttaggaggt aaaaatgata
     1321 aacgatatta aaaataaatt caaaaattac aaaccatata taaatggata caagaatatg
     1381 aagagagcat ctgttttaat tccaataata gaaataaata acacacacta tatattattt
     1441 gaagttcgct caaaaaattt gaagcatcaa cctagcgaaa tttcttttcc aggtggtaaa
     1501 atagaatcag gtgagactcc atatgaagct gttattagag aaacttgtga ggaacttgga
     1561 acttttagtg acaatataga agtaatatca agtcttgacc tccttataac accagtaaag
     1621 tttatcatac atccatactt aggatatata aaaaatatta ataatttaaa tataaacaag
     1681 gatgaagttg accatacttt tttggttcct ataaaatatc ttttagagaa tcctccaaat
     1741 acttatagta atactgtaaa agtttttcca gatgaaaatc taccatatgg ttcaattcca
     1801 aaccaaaaag attataaatt tgaaagtgga agttatccta ttctgtttta taaatataaa
     1861 gattatgtaa tttggggaat gacagcaaaa atactagaaa actttttaaa ttttttatca
     1921 gaataacact cataataaat catcaaaact tttacataag aaatttataa aagtttctaa
     1981 atttaacaat tctaaaatat caataactat attttgtagc acttttttat tttattattg
     2041 tttgaatatt tgatggtttt caatgcaatt tcaacataaa tactatgtat tagtgtatac
     2101 tgttgatgaa ttaaagtatg ataatctatt taataaagta tgaagtaaat gattttaatg
     2161 tgtctttatg gtaaaattac taataagtaa taaaattaat taaaatatat ggaggtgctt
     2221 ataatgtttg ctgtaggatt tgttgttata attgcacttg cctttttcat tttaagtgta
     2281 ctattattta atggaaaagc cagtatgcta ctagcaggtt ataactctat gagtaagaaa
     2341 gaaaaagcac gatacgataa aaaaaaatta tgtagaagtt ctagcattgt tacttttatt
     2401 gtctctataa tgctatttat tatggcattt ttgggatata gaatagagag tggtcaaatg
     2461 gacgaaaaaa aaatggttcc cttttctata atatttataa tagtcatact ttccagtgta
     2521 ttttttaata tcctctactc taacaaaaaa tgtaaaaaat aaatagttgt aagttgagca
     2581 gttaattata aaagattgac tgctttttca tgtatattga ctactcttat taaatatttt
     2641 aacatttaaa aatctatgta aaaataatga atatagtaca aacttctaat aatgatttta
     2701 cctttgtggt tacagtccac tatatataat ttaatatgat aacatggaat tactaatgac
     2761 aactaagtaa gcttttataa aatataataa aacaaaaata gatgtacaat gaaattatat
     2821 cttatatcaa atttattttt attaatagca aaaacttaca ttagcataac atatgacaaa
     2881 ttcgcttaaa ataaaaattt ttaatataat ttgttctata gtacatctat ttcgattagt
     2941 ttacataatt ttattatttt gtacaatatt aattatttac taatatccca tttaatgaaa
     3001 aagttctagg ttcagtaatt ttgaccataa ctaatttacc aattaaatca tcatttccac
     3061 cttcgaaatt tacgagttta ttttgtctag ttctaccagc aaacttattt tcattatttt
     3121 tacttcttcc ttcaactaga acttcaacta ttctatcttt atatccatca tttatttctg
     3181 cagaaatcct atttacagct tctaacaccc tattaaatct atcatgcttt atatcttctg
     3241 gtatttgatt ttccatttta gcagcaggag ttccctgtct tttagaataa ataaatgtaa
     3301 aagctgaatc atatctaact ttttcaacca catctaaagt gtccaataaa tcttcttctg
     3361 tttctcctgg gaaaccaatc attaagtctg tagaaaaagc aatacctgga acttcctttt
     3421 tagctttttc tattatttct aaataatatt cctttgtata atgtctattc atttttttca
     3481 aaaggctact gcttccacat tgtattggta gatgtaaaaa ctcacacacc ttatcacaat
     3541 ctctaattgc gtatattact tcatcagata tatcttttgg atgagatgtc ataaatcgaa
     3601 ccctttcaat tccttctata tcattcactt ttcttaaaag ttctgagaat gaaactggat
     3661 tttcaagtgt cttaccataa gaatctacat tttgtccaag taaagtgatt tctttcgttc
     3721 catttgcaac aagctctttt atctcgttaa ttatatcttc aggagttcta cttctttctc
     3781 ttcctcttgt gtatgggact atacaatatg tgcagaaatt attgcatcca tacattatat
     3841 ttacaaatgc ttttaattca aattttctat tacttctaag accttctatt acttctccat
     3901 ccacatccca tacatccact aacattttgt cagaatttat tgattctgta agtaattgcg
     3961 gaaatttata tagattatgt gttccaaata taaggtctac atgtttatat ttttttctta
     4021 attcctctac aacatgtggt tgttgcatca tacatccaca tacagctatt ttcatatctg
     4081 gattttttcc ttttagatgt tttaattgtc ctaagttacc atacactttt agctctgcat
     4141 tttctctaac tgcacaggtg ttatatatta ttaaatcact ttcttcaacc atcattgaca
     4201 tttggtatcc catctcttct aacatcgaac ataatttttc tgaatcatgt tcattcattt
     4261 gacacccaaa ggtttgagta aacacgagtt ttctctttcc agttctgttg aagtattctt
     4321 cattttgtct gtgtatttgt tctatatatt tatcttgttc ttgtattttc tctataggta
     4381 ttgttacttg ttttcttttg ctcacgtcta ttcctcctag ttaatttctt atattacaca
     4441 taaccttaat tatatcaaat aagaaaaatc ttttaaagat attattattt ttctagttgt
     4501 gttttaatag taaatctaat gatttatata tactatattt ctaaaatata aaatcatata
     4561 attattataa taataaaaag atgttttaaa ttaaaactta aaacatcttt ccttaaataa
     4621 aatatatgat ttactattaa atacaattac atctattcat tttcaattaa ataacaatca
     4681 tttaatatta aatattgacc ttcctcaacc gttatatatc ttcttcctga aaaagtatca
     4741 ttagtaacta ttccctctgc attacctaag ctatcatttc taacttcata atatgcatca
     4801 gaagaatttg acattataat atattctcca gcaggaattt ctaatccaat tttatacata
     4861 ccatttccaa acttaccatt cttggccttc tgggctttag catactttgc taatgtcatt
     4921 gttgaatcct caattttaat atattgacca ttctttaagg tgatatatct agtcccacta
     4981 aaaatatcat ttgaaagtat actatcagca ttacctgtac tatcacttgt aacttcataa
     5041 taagaagcat aacttcctga gtttgaagta attaagtatt cacctgctga tatatctttt
     5101 ccaaccttat acattccatc ttttatagtc tttgtagtag gtttaacatt ttttacaacc
     5161 ccagtattaa tatctccaat tccatttgtt atatttaaac actgttgtac tatatttgaa
     5221 tctatatatc ctatagaagt tatcttctta gcatttttta aaatgctttt attgctacca
     5281 tcattaacta aaactattgg accatgtttt gataaagaag atcctacaag tgcatttgtt
     5341 aattcataag ctccagctat ataaaactct ctagcatcct tataaaactt atttattatt
     5401 gctttattag tttcaaaacg agtactccct cctagtctag tagatttagt attgtttact
     5461 aaagtggtac tcattgaagc tgttcctcct attacataag actttaaacc actagttgaa
     5521 aaaggtatac tttgtccatt agtaagaatt attggtgctt tatctcttgc tgctacagat
     5581 gcaatactta tagcatctgc ttctccttta tatgcattgg ttagcataac agtattgact
     5641 ttttttattg aatttatttc ttttgcaaca ttataacttg tttttattct gtcatttccc
     5701 ttaattctta caactttcat tttcttagat tttaatgaat tttcgacaga tgtactaata
     5761 gaatatgttc ctccaattat ataaactttt gatacatttt ttaatcttgc agatgtctca
     5821 gttggtatag tatttttttc agttaatagt ataggggcat ttaaagctcc tgctagtcca
     5881 cttgcactta atccatctgc aatagagtta ctagtattaa ttaatatagc agttttatat
     5941 gcatttttat ctgctatttt gcctgctatt tcatacttat tttttccttg tattttttct
     6001 acagatgata ttgcatctac taattgaata tttgcaaata caaaagaaat acttaaagtc
     6061 aatgttaata ttcttttata gaatttcata ataaaattgt ccccctcata ataaattttt
     6121 aatatataaa tacatattct taaattagtt atgtacttaa caaacttatg taaaattgca
     6181 aattatatta agcatattac aaataaatat taatacaatt attcatttag ctataattgt
     6241 atttacattt atattaaa
//
LOCUS       DAGQWR010000187         6254 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.190, whole genome shotgun sequence.
ACCESSION   DAGQWR010000187 DAGQWR010000000
VERSION     DAGQWR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.190"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            272..1216
                     /locus_tag="MIW95_003520"
     CDS             272..1216
                     /locus_tag="MIW95_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBY3152816.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1238..1864
                     /locus_tag="MIW95_003521"
     CDS             1238..1864
                     /locus_tag="MIW95_003521"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBY3152817.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1898..2947
                     /gene="hisC"
                     /locus_tag="MIW95_003522"
     CDS             1898..2947
                     /gene="hisC"
                     /locus_tag="MIW95_003522"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBY3152818.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2950..3528
                     /gene="hisB"
                     /locus_tag="MIW95_003523"
     CDS             2950..3528
                     /gene="hisB"
                     /locus_tag="MIW95_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBY3152819.1"
                     /translation="MRIWKVERNTFETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3530..4135
                     /gene="hisH"
                     /locus_tag="MIW95_003524"
     CDS             3530..4135
                     /gene="hisH"
                     /locus_tag="MIW95_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBY3152820.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDITLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELVAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4132..4854
                     /gene="hisA"
                     /locus_tag="MIW95_003525"
     CDS             4132..4854
                     /gene="hisA"
                     /locus_tag="MIW95_003525"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBY3152821.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGINTKIIEDIANNIDIPIQVGGGVRDKGKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSDVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4836..5594
                     /gene="hisF"
                     /locus_tag="MIW95_003526"
     CDS             4836..5594
                     /gene="hisF"
                     /locus_tag="MIW95_003526"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBY3152822.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKNMGVEVRL"
     gene            5622..>6254
                     /locus_tag="MIW95_003527"
     CDS             5622..>6254
                     /locus_tag="MIW95_003527"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBY3152823.1"
                     /translation="MDNKCNNVYSDEVEEFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQKLWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMEPEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEID"
BASE COUNT         2539 a          534 c         1246 g         1935 t
ORIGIN      
        1 aaattatttg ggaggaaatt atcatggtga gattaaatat agtaaaatta aatacaaaat
       61 taaatcatca ccatcatcat agattaaagg gattgtaata tgaaattaat tcatagacac
      121 aagccctgtt tttagtgtaa ttggtcttgt atctatgatg ggaagtaatt ttaggagaaa
      181 attatagcca tataggtata aagcctatat ggctttttta ttgttttaaa tttgcaaata
      241 taagagtaaa tttaatgaag agggtgagaa tgtgaaattt ttgaggattg aagatgaagt
      301 tatttattct aaaaaaagat ataaattaaa aagggaaata gataaacttt ttttggatga
      361 agaatatttt caaatagaac cacaattatt tgaagaatat gatgaattta caactataaa
      421 taataaaatt ccagaagaat ctatggtaaa ggttgtaaat ggaaaagtaa tggtacttag
      481 agctgatata actacaagta taataaaaag ccttgtacca agatgggaag atggtttaaa
      541 gcttaagctt ttttataatt catcaatata taaaaataaa aatacagttg gaataaaaga
      601 gataagacaa ataggttgcg agtatctagg agaagcctct gtagaagcag atagagaagt
      661 agtgaaatta gctcttaaga tacttgaaaa atacaataat aattttattt tagaggttgg
      721 gagtagcaag tatattcatg gtttgttaga agaattaaat ttaaataaga attgtgaaaa
      781 tcaaattaaa aatttactat atacgaagaa cactcatgaa ttaaaagtat atattgaaga
      841 actgaaaatt aagcgtgaag taaaagagct attatcaaat attttaagct tgcaagggaa
      901 tttatacaac gttattgaaa aatcaaacaa gttctattgt aataataaga tgaagcaagc
      961 tcttgaagaa ttaaagcaag taaataattt aattgaggaa tgtaattttt tagataaggc
     1021 tagatttgat ttatcaatga ttactatgct tgactattat gaaggaatta tgtttagagg
     1081 atactaccca aactcatata aagaaattct tagtggtgga agatatgatt ctttaactaa
     1141 agagtatgga aaagaaattc cagccattgg atttacacta agtgttgatg agttaatgaa
     1201 gtatgtatat aagtaaagtt tagaaggagg gattgctttg gattatttga ctatagcact
     1261 agcaaaagga cgaattgaag gggaatcatt taaaaaattt aaaaaaatgg gtctaggaga
     1321 tagtatagac acagatacga gaaaactaat ttttaaagat gaagaaaata aaattatata
     1381 tatacatgta aagccatcag atgtagttac atatgttgaa aaaggagtcg ctgaccttgg
     1441 tatagcaggt aaagatacta ttttagagaa tgagacagat gtatatgaaa tatatgattt
     1501 aggttttgga aaatgtaaat ttgctgtagc aggattaaag ggagatagca tatatagaga
     1561 agatgaatat ctaaaagtgg caacaaagta tcccaatata gcaaaaaagt attttaaaga
     1621 gaaaggtcaa aaaattgaaa taataaaatt aaatggttct gtagaactag ctccaatagt
     1681 agggttgtca gatgtaattg tagatatagt tgagactggc aatacactta aagcaaatgg
     1741 tcttgaaata ttagaagaca tatgtaatat aagtgctaga attatatcaa atagagctag
     1801 ctatagattt aaatatgaac aaattcagaa tataattcgt ttatttgaag aacttgataa
     1861 ttaaaatata attgaacttt ataaggaggg atttaagttg agagaaaaag aaagtataag
     1921 agagttaagg ggatatgaac caaatcacgt aaactgtaaa gtaaaacttg atgcaaatga
     1981 aggaagtaag aggttgttta aataccttat taaagaaatt tcagatagtg atatagattt
     2041 aaatttatat cctgaagatt cttatagtga tttgaaagaa tctataatag attacataaa
     2101 tatttctgga gtaaataaga aaaatcttct agtaggaaac ggttcaagtg aaataattga
     2161 tttaataata catacttttg tagataaaga tgaagtaata ttgagtttct ctccaagttt
     2221 tagtatgtat tctatatata gtcagataaa tgggtctaaa tttataggtg ttgaaagtga
     2281 tgaaaattta gttataaata tagataatgt gattgaaaaa gtaaaggaga ataatcctaa
     2341 aatagtaatt gtatgtaatc ctaataatcc aactggaaca atattaaaga gggaagagat
     2401 aataaagctt ttagattcaa ctaatagtct agtggtttta gatgaagcat atatggattt
     2461 tggagaagaa agcatgttaa gtgatgtttt taagtatgat aatctaatag ttcttagaac
     2521 tttatcaaag gcatttggat tggctggaat aagaacagga tatatgttat ctaatagtag
     2581 tttaataaat tctgttgaga aagtaagacc tccatataat ttaaattcat tatcagactt
     2641 tattgctaca agagcactta gaaataaaga tgtggtaaag gcttatataa aggaagtaaa
     2701 agaggaaaga gaagttttat atgaagaaat gattggtatg ggaattaaag catacaaatc
     2761 acaagcaaat tttatattat tttattctga aatcgaaaat ttatcacaaa aacttattga
     2821 tagaggagtt ttaataagaa aatttggtgg aaagttagaa aattattaca gagttacaat
     2881 tggtgataaa gaagaaaatt ctatgtttgt tggagctata agagatattt tgaaaaagga
     2941 gaaataggta tgagaatttg gaaggtagaa agaaatactt ttgaaacaca gattttagta
     3001 gaattaaata ttgatggttc tggtaaagct gaaatagata ctggaatagg atttttagac
     3061 catatgctta ccttaatgtc atttcatggc aagttcgatt taaaagtgat ttgtaagggt
     3121 gatacatatg tagatgacca ccatagtgta gaagatatag gtatagctat aggtgaagca
     3181 tttaaaaatg cactaggtga caaaaaagga atacgaagat attctaatat ctatatacct
     3241 atggatgaat ctctatcaat ggtagcaata gatataagta atagacctta tcttgtattt
     3301 aatgctaaat ttgataccca aatgattggt agtatgagta ctcaatgctt taaagaattt
     3361 tttagagctt ttgtaaatga atcgagagta acattacata tcaatctctt atatggggaa
     3421 aatgaccatc ataagataga aagtattttt aaagcatttg caagagcttt aaaagagggt
     3481 agtgaaatag tttctaatga gatagcatct tcaaaggggg ttttgtagaa tgaatataat
     3541 agtagactat ggtcttggaa atatagattc tgtgtctagg ggttttagaa aagctggaat
     3601 agaaactaaa atttctagtg atatagatga aataaaacag gcagattcac ttatacttcc
     3661 aggtgttgga gcttttagag attctataag tgccttagat aaactaggat tgataccaat
     3721 aataaaggag catgtttcta agggtaaatt tatgataggt atatgtctag gaatgcaatt
     3781 actttatgaa aaaagttatg agtatggaga atatgaagga ttaggactta taaaaggaag
     3841 tattgataaa ctggatataa ctttaaaagt gccacatatg ggatggaata atctaaaatt
     3901 taataaggca aatgatgata tacttaaata tataaacgaa gatgattatg tatacttcgt
     3961 tcactcatat tatgcaaatt catcaaatga agaactagtt gcattttcag aatatgaaaa
     4021 gaaaatccct gctattgtta gaaaaggcaa tgtatatggt atacaatttc atcctgaaaa
     4081 aagtggagaa gtagggttaa atatattaag agcatatggg gagatgataa aatgataata
     4141 tttccagcaa tagatataaa agataataag tgtgtaagac tcacacaagg agaatttgac
     4201 aaagtaaatg tatattatga taatccatta gaagtagcat ataaatggaa aaatgaagga
     4261 gcagaatata tacatatagt agatttaaat ggtgctagaa gtgaatttgg tataaataca
     4321 aaaataattg aggatatagc aaacaatata gatataccta ttcaagttgg tggtggagtt
     4381 agagataagg gaaaagtcaa aagtttaata aatgctggtg ttactagagt aatattaggt
     4441 agtatagcga ttgaaaattt gaatttggtt gaagaattgg tcaatgaata taaagagaaa
     4501 attgtagttt ctatagacgc taaagatgga aaagtagcag ttaggggatg ggaagttgta
     4561 agcgatgtag actcattaac attatgtaaa caacttgaaa agataggtgt acaaactatt
     4621 gtttatacag atatatcaaa agatgggatg ttgcaagggc caaattttga catatatgaa
     4681 agaatagcaa aggaaacgtc attaaatgtt attgcttctg gaggcgtaac ttctatagaa
     4741 gacgtaaaaa gactaaaagc tatgaactta tatggtgcaa taataggtaa agcgctttat
     4801 gataaaaaga tagactttaa ggaggcacaa caattatgct tactaggaga ataatacctt
     4861 gtctagatgt gagaaatgga agagttgtaa agggaaaaaa atttaaagat atagtagatg
     4921 ttgatagtcc agaagtactt ggaaaatttt atagtgattg tggtgcagat gaacttgtat
     4981 tctatgatat aactgcatca aatgaagaga gaaagacatc tttagaattt gtgacaaaag
     5041 ttgcagaaaa tataaatata ccattttgtg ttggtggagg agtaaataaa ttagaagatt
     5101 tcactgatat tttaagaaaa ggtgctgata aggtttctat aaattcatca gcagtcaaaa
     5161 atcctgaatt aataagggaa gcttcattaa aatttggagc acaatgtgtt gttttatcga
     5221 tagatgcaaa gaaaaatgaa gagggttcat ggagcgtata cgtaaaaggt ggaagagaaa
     5281 aaactaatct ggatgcaatt gaatgggctg ttaaaggtgt agaacttgga gcaggagaaa
     5341 ttgttgtaaa tagtatggac gaagatggaa tgaaaaatgg atatgatata gaattattat
     5401 caaagataac ttctttagta aatgtacctg tcatagcatc tggaggcgct ggaaaaaagg
     5461 aagattttta tgaggcagtt aataaatcta atgtagatgg aattttagca gcatctgttt
     5521 ttcattttgg cgagataaaa ataaacgatt taaagaaata tttaaaaaac atgggtgtag
     5581 aagtaagatt ataagttaaa tactaaaagg agattagaaa tatggataat aagtgtaaca
     5641 atgtatacag tgatgaagta gaggaattta taagaagtat aaagtttgat gataaagggt
     5701 tagtccctgt tgttgttcaa gaggtagtta gtaaagatgt actgatgtta gcatatatga
     5761 ataaagatgc tataaagaaa accttaaaag ataaagtagc ttgctatttt agtagaagta
     5821 gacaaaaact ttgggttaaa ggtgagacat ctggtaatac tcaaaaagta gttaagatgt
     5881 cttatgattg tgatgtagat actatactac tttttgttga acaaactggt gtagcttgtc
     5941 atacaggcaa ttattcttgt ttttacagag acttgtttga tgatagttca aaaatggagc
     6001 ctgaagtaca aacaaatata ttgaaagaat tatatgattt aatcaatgaa agaaaaaata
     6061 atccagttga aggttcttat actaattatc tttttgaaaa aggaatagat aagattctta
     6121 aaaaagtagg tgaagagagt agtgaggtaa ttatagcatc aaaaaatacc gataaaagtg
     6181 aacttattta cgaaattagc gatttagtgt atcatacatt ggttttaatg atagaaaaag
     6241 gtgttgaaat tgat
//
LOCUS       DAGQWR010000188         6199 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.191, whole genome shotgun sequence.
ACCESSION   DAGQWR010000188 DAGQWR010000000
VERSION     DAGQWR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.191"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            126..686
                     /locus_tag="MIW95_003528"
     CDS             126..686
                     /locus_tag="MIW95_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY3152824.1"
                     /translation="MFCSNCGAEITGIGKFCSSCGVAVETEIIEDNNIKSNDLIVDAN
                     GIEINMTEIYRKYRKEKVNAIKNVMEISGLNIKEAKKIVDFSFEELKINFIDDTMSNS
                     EKEKIIQNQNRKNNIEKAQQESVACCPKCGSTSLTAQKKGFGIGKAMVGASLTGGIGL
                     VAGNLGAKKVRVTCLNCGKQFWAGKK"
     gene            764..3052
                     /locus_tag="MIW95_003529"
     CDS             764..3052
                     /locus_tag="MIW95_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBY3152825.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMTTAMNIVLSSFEQLQRTSSNAVN
                     ANSIIRAREELARAEAGFDRLERQIRESDNQQRKLNEDINKGASSTDRLVGSAKKLAA
                     TYLGIRTLGGLGNLSDQMTSTNARLGMINDGQQSDAGLNKMIFQSAERSRASYLDTAQ
                     IVSRIGMNAGKAFSSTKEIVGFAEQLNKKFVIAGASTEEMNSALLQLTQGLSSGVLRG
                     EELNAVFESAPNIIQSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAAAEQTNAEFE
                     KMPYTFSQIWTSIKNNAIMIFGVIQKKIEQSMSSKGFRTFIDNFINSLYVLGNVAYNI
                     FNEIISILGSPFFQAFVNAIIVGVSLIVQALGWIITQALNIANVFAQNWSIIAPIVLG
                     VAAAMLVYNNALLLSIANKVKDIALSAKSLVMSFAHIVAESYRAAALVATTIAQDGLN
                     AAMAACPITWILYGIIAIVVAFFVAIAIFNHFAGTSVSAIGVVAGAISVAASFIGNLF
                     IATGNLIIDIVALIYNTLAGFAEFFANFLDDPIGSVIRAVSGMANAVLGIIRSIASAF
                     DTVFGSNLADAVSGWQDKLQGWTDKVAGEAKIKVERMDPNKLHFDRFNYGKAWDAGYK
                     WGDKLETNIKDKFDISKMAEDAKKKLGLDDLWDKKYGLGDGFGSAGLNSPLNDAAKGA
                     KDTAGNTAKMAKTMDKSQEDLKYLRDIAEQETINRFTGVNIKIDMNNTNNISKDNDLD
                     GIVNVLTEKLNDAMAVSAEGIV"
     gene            3069..3770
                     /locus_tag="MIW95_003530"
     CDS             3069..3770
                     /locus_tag="MIW95_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBY3152826.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWAICQKQLGNGSLYKKVYELNKTMMDKANKGKK
                     LSKYTIYKGQVLRLV"
     gene            3763..5733
                     /locus_tag="MIW95_003531"
     CDS             3763..5733
                     /locus_tag="MIW95_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBY3152827.1"
                     /translation="MSDDLVLANDRDIRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFKLKYNVIEDTKYKISRVEENKTLFDMVLTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQLFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLSLGDIDLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKNEKQNGPAQSTTSKEDTDMANKINKLLKGKLSNTGNIFVKYSNAYK
                     VNPALMAAISMHESARGTSNIANTKNNFFGMKKNGDYMSFSSVDEGIKRGISNLSRNY
                     IHIGRKTLESIRNKYSSSSDKEWVKCVGAFYKQITGSTYNSNSAGTGVGSNEEAEKNL
                     KDLTYQVQNNNSNTSTNNNNKVSKVIQEAKNQLGKPYKWGGNGPKSFDCSGLMVWAFK
                     RGAGINLKRVSADQSKDSRGKLLCNINDVKAGDLVFFAYNKGKGNVHHVGLYIGNDQY
                     IHAPQTGDVVKISSLSGRQKKKHDFARARRFF"
     gene            5747..6007
                     /locus_tag="MIW95_003532"
     CDS             5747..6007
                     /locus_tag="MIW95_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBY3152828.1"
                     /translation="MSQELLQIIKKAAMDAVETSNPMQIAFGTIESVNPLIVKIEQKA
                     SFEEFFLIQTETFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            6012..>6199
                     /locus_tag="MIW95_003533"
     CDS             6012..>6199
                     /locus_tag="MIW95_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBY3152829.1"
                     /translation="MLPTDNIDYDIEDVSIINFDVRQEPSKTFKLNIEKNRVDGICDD
                     VEALKQTIFLILNTERYE"
BASE COUNT         2452 a          728 c         1237 g         1782 t
ORIGIN      
        1 caaaaatact aaaaacactt actaatgtag gtgttttttt aatgaaagga tgtgatgata
       61 atgtaaaatt tttactaata tagtataata ttcttataaa attattatac tgggggggaa
      121 atgttatgtt ttgttcaaat tgtggtgcag aaatcacagg cataggcaag ttttgttcaa
      181 gttgtggggt tgctgtagaa actgaaatta ttgaagataa taatattaaa tcaaatgact
      241 taatcgttga tgctaatgga atagaaataa atatgactga aatttataga aaatatagaa
      301 aagaaaaagt aaacgcaata aaaaatgtaa tggaaataag tggtttgaat ataaaggaag
      361 caaaaaaaat agtggatttt tcttttgaag aattaaaaat taattttatt gatgatacta
      421 tgagtaattc agaaaaggaa aaaataatac aaaatcaaaa tagaaaaaat aatattgaaa
      481 aagctcaaca agaatcagtt gcttgttgcc ctaagtgtgg ttcaacatcc ttgacagctc
      541 aaaaaaaagg ttttggtata gggaaagcaa tggtaggagc tagtctaact ggtggtatag
      601 gtttagtagc tggaaattta ggagcaaaga aagttagagt tacatgcttg aattgtggga
      661 agcagttttg ggcaggcaaa aaatagatgt attaattata aaaacactta ctaatttagt
      721 aagtgttttt attatctaaa ttaacagaaa ggagagtgaa aaaatggcta caatacagac
      781 atcaattcgc atatttgatg gaatgacacc tgcgtttcgg catatgacaa ctgctatgaa
      841 catagtactg agttcatttg agcaattaca aagaacttct agtaatgcag taaatgctaa
      901 tagcataata agagctagag aagaactagc aagagcagaa gctggatttg atagattaga
      961 acgacaaata agagaatcag ataatcaaca gcgaaaactt aatgaggata taaataaggg
     1021 tgcaagttct acagatagat tagttggaag tgcaaagaag ctagcagcaa cttatttagg
     1081 tataagaaca ttaggaggtc taggaaattt aagcgaccag atgacaagta ctaatgcgag
     1141 acttggcatg ataaatgatg ggcaacaatc agatgctgga cttaataaaa tgatatttca
     1201 atcagctgaa aggtcaagag catcttactt agatactgca caaatagtga gtcgtatagg
     1261 catgaacgca ggaaaggcgt ttagcagtac aaaagaaatt gtaggttttg cagagcaatt
     1321 aaacaaaaaa tttgtaatag caggcgcaag tactgaggaa atgaactcgg cattgttaca
     1381 gctaacccaa gggttgagtt ctggcgtatt aagaggtgag gaactgaatg ctgtgtttga
     1441 gtcagcacct aacatcatcc aatcgattgc agattatttg gacgtggaca taggaaaaat
     1501 aagaggaatg gcatcagagg gaatgttaac agcagatatt gtaaaaaact cattacttgc
     1561 agcagcagag cagaccaatg cagagtttga aaaaatgcct tacacatttt ctcaaatttg
     1621 gacttcaatt aaaaataatg caatcatgat atttggtgtt atacagaaaa aaattgaaca
     1681 gtctatgtct agtaagggat ttcgaacctt tatagataat tttataaact ctttgtatgt
     1741 acttggaaat gttgcttata acatttttaa tgaaattata agtatattag ggagcccgtt
     1801 ttttcaagca tttgtaaatg cgattattgt aggtgttagt ttaatagtgc aggcactagg
     1861 ttggataata acacaggcat taaatattgc taatgtgttt gctcagaact ggagtattat
     1921 tgcaccaata gtacttggag ttgcggctgc tatgttagtg tataacaatg cgttattact
     1981 tagtattgcg aataaagtta aagatattgc actatctgcc aaatctttag tcatgagctt
     2041 tgcacatata gtagcagagt cttatagagc agcagcttta gtagcaacta caatagcaca
     2101 agacggatta aatgcagcaa tggcagcttg tcctattact tggattttat atggaattat
     2161 agctatagtt gttgcatttt ttgtagctat agcaatattt aatcacttcg caggcactag
     2221 tgtatctgct ataggtgtag ttgcaggtgc aatatcagtt gcagcctctt tcataggaaa
     2281 cttatttatt gcaacaggaa atttaattat agatattgta gctttaatat acaatacttt
     2341 agcaggcttt gcagagttct ttgctaattt tttagacgac ccaattggct ctgttataag
     2401 agcagtatcc ggaatggcta atgctgtatt aggcattata agaagtatcg caagtgcatt
     2461 tgataccgtg tttggttcaa acttggcaga tgcagtaagt ggatggcaag ataaattgca
     2521 aggttggact gataaagtag caggagaagc taaaataaaa gtcgaaagaa tggaccctaa
     2581 taaactgcat tttgacagat ttaattatgg aaaagcatgg gacgcaggat ataaatgggg
     2641 agataagtta gaaactaata taaaagataa atttgatatt agcaaaatgg cagaagatgc
     2701 aaagaaaaaa ttaggattgg atgatttatg ggataaaaaa tatggattag gagatggatt
     2761 tggttcagca ggattaaact ctcctctcaa tgatgcagca aaaggagcaa aagacactgc
     2821 gggaaataca gcaaagatgg ctaaaacaat ggataaaagc caagaagact taaaatactt
     2881 aagagacata gcagaacagg aaacaatcaa tagatttaca ggagtaaaca taaaaattga
     2941 tatgaacaac acaaacaaca taagtaaaga taatgactta gatggaatag ttaatgttct
     3001 aactgaaaag ttaaatgatg caatggctgt ttcagccgaa ggaatagttt aggaaggaga
     3061 gtgagaaaat ggcttatgat ttttacctag atggagtaca actaccaatc gcaccaccaa
     3121 agcttgaagt caaagtgaca aataaaaaca agacagttga tttaataaat gttggagaag
     3181 taaatatatt aaaaaaagaa ggattatctg aaataagttt tgaagcagaa tttacacata
     3241 ataaactacc attttatcgt ggaactttta gggatgttca attcttttta agtaaactgg
     3301 aactactaaa aactgattgt aagccatttc aatttattgt atcgagggaa ttaggtaata
     3361 aagtactatt taacactaat ataaaagtat ctcttgaaga gtatgctatt tcagaagatg
     3421 cagataatgg ctcagataca aaagttgcaa taaaattaaa acaatataga gattactcaa
     3481 ctaaaaagtt agttcctgca actcctgaaa agacaaacta tggtaggact ccccctccag
     3541 tcatgaaacc aaaagaattt agaccagatt catccaataa gccaaatggt aaaacatata
     3601 cagtaaaagc aggggattct ctttgggcaa tttgtcaaaa gcaattagga aatggttcgt
     3661 tatacaagaa agtatatgag ttaaataaaa caatgatgga taaagctaac aagggaaaaa
     3721 aattaagtaa atacaccatc tataaagggc aggtgttgag gcttgtctga tgatttagtt
     3781 ctggcgaatg atagagatat aaggctagtt attgcacatt gggaagattt ctacgaacct
     3841 gtagttttgg atggtatcac atgggaaata gagagacgag gaacaccatc taaacttgaa
     3901 tttacaatag tcatggatga tatattagag ttttgtgaag gtaactctgt aagactatat
     3961 tacaaaggtg taggtatatt ctatggatat atatttcaga aaaagagaga taaagaaaat
     4021 catatcaaga tagttgctta tgaccagcta agatatttta agaataaaga tacttatgta
     4081 tatagtaata aaacagcaag tgaacttgta aagatgttgg ctaaggattt taaattaaaa
     4141 tacaatgtca tagaagatac aaaatataaa atatcgagag ttgaagaaaa taaaacactc
     4201 tttgatatgg tcttaactgc actagatgat actctaagag agaaaaagga aatgtatgtt
     4261 ttatatgatg attttggaag aataacatta aagaatgttg cttctatgaa acttgatacg
     4321 gttatgaaca atgatgtaat tgaggacttt gactacaatt cttctattga tagtgatact
     4381 tacacaaaga ttaaacttgt gagagataat gaagaaacag gaaaaagaga tgtgtatatt
     4441 gctcaagact caacgcatat gaggagttgg ggaatactac aactatttga tacagttgat
     4501 aaaaatatga gtgaagcaga gataaaacaa aaatgtgata tactcctaaa actatataat
     4561 aagaaaacta agtcattaag tttaaaaaat gtgttaggag atattagagt aagagcaggt
     4621 tgtttagtac ctgttttttt gtcgttagga gatattgatt tacaaaatta tatgttagtt
     4681 gagaaagtaa aacatacatt tgaaaataac agtcatttta tggacctaac tttggttgat
     4741 ggagacgaat ttgcttctta ttcttcatca agttatagta gtggaaatac taataataaa
     4801 aatgagaaac aaaatggtcc tgcacaaagt actacaagta aagaagatac tgatatggct
     4861 aataagatta ataaactact taaaggtaaa ttatcaaata caggaaatat atttgttaaa
     4921 tattcaaatg cttataaagt taatccagca ctcatggctg ctatatctat gcacgaatca
     4981 gctagaggga cttcaaatat tgcaaatact aaaaataatt tctttggaat gaaaaaaaat
     5041 ggagattaca tgagtttttc tagtgtagac gaaggaataa aaagaggtat aagtaattta
     5101 tcaagaaact atatccatat aggacgaaaa actttagaaa gcatcagaaa taaatattct
     5161 tctagttcag acaaagaatg ggtaaaatgt gtaggtgcat tttataagca aataacagga
     5221 agtacttata attctaatag tgcaggcaca ggagttggaa gtaatgaaga agcagaaaag
     5281 aatttaaaag atttaactta tcaagttcaa aacaataatt ctaatacatc aacaaacaat
     5341 aataataaag taagtaaagt tattcaagaa gcaaaaaatc aacttggcaa gccttacaaa
     5401 tggggtggta atggtccaaa gagttttgac tgtagtggtc ttatggtgtg ggcatttaaa
     5461 agaggtgcag gaataaatct caaaagagtt tcagcagacc aatcaaaaga tagtagagga
     5521 aaactattat gtaacataaa tgatgtaaaa gctggtgatt tagtattctt tgcatacaac
     5581 aaaggaaaag gaaatgtaca tcatgttgga ctatatatag gaaatgacca atatattcat
     5641 gctccacaaa ctggtgacgt agtaaaaata agtagtttaa gtggtagaca aaagaaaaag
     5701 catgattttg caagagctag aagattcttt taagtgaggt gataaagtgt cacaagaatt
     5761 attgcaaata attaagaagg ctgcaatgga tgcagtagaa acaagcaatc caatgcaaat
     5821 tgcatttgga actatagaaa gtgttaatcc tttgatagtt aagatagaac aaaaagcatc
     5881 ttttgaagaa ttttttctaa tacaaacaga gacttttaaa agatatacag ataaaaaaat
     5941 aggggataaa ttagtcttaa ttaggatgca aggaggacag caatatttga ttttagatag
     6001 gatgtgatga aatgttacca acagataaca ttgactatga tatagaagat gtatcgataa
     6061 ttaattttga tgttagacaa gaaccaagta agacgtttaa attgaatata gagaaaaata
     6121 gagtagatgg tatttgtgat gatgtagaag cattaaaaca aaccattttt ttaattttaa
     6181 atactgaaag gtatgagca
//
LOCUS       DAGQWR010000189         6183 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.192, whole genome shotgun sequence.
ACCESSION   DAGQWR010000189 DAGQWR010000000
VERSION     DAGQWR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.192"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(232..1005)
                     /gene="srlD"
                     /locus_tag="MIW95_003534"
     CDS             complement(232..1005)
                     /gene="srlD"
                     /locus_tag="MIW95_003534"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBY3152830.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIETEFGCKSVGYGCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            complement(1106..1471)
                     /locus_tag="MIW95_003535"
     CDS             complement(1106..1471)
                     /locus_tag="MIW95_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBY3152831.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTNDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            complement(1512..2471)
                     /locus_tag="MIW95_003536"
                     /pseudo
     CDS             complement(1512..2471)
                     /locus_tag="MIW95_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
     gene            complement(2684..3874)
                     /locus_tag="MIW95_003537"
     CDS             complement(2684..3874)
                     /locus_tag="MIW95_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBY3152832.1"
                     /translation="MSNVKRKDGKNRNLRNGESQRKDGRYVYKYTDIYGKPQFVYSWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLNDGIDTVGGKMTVCQLYDKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSEKGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEQKVILTPEQEERLLAFMEKDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLKDSEIGYYIETPKTKNGKRELPLTE
                     RAYQAIQRILKSRGKAQPLIVGGYSNFLFLNREGLPKVAGNYEGMVRGLIKKYNKYHT
                     DKLPNITPHSFRHTYCTNMANRGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            complement(3952..4155)
                     /locus_tag="MIW95_003538"
     CDS             complement(3952..4155)
                     /locus_tag="MIW95_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBY3152833.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENIDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            complement(4530..4769)
                     /locus_tag="MIW95_003539"
     CDS             complement(4530..4769)
                     /locus_tag="MIW95_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3152834.1"
                     /translation="MNNTRPSFYLISSAVDGNVNAIEKILALYDPYISKCCLRPFYDK
                     YGNVCIVVDMELKGRIREALIKMILDFDIPLETEE"
     gene            complement(4762..5190)
                     /locus_tag="MIW95_003540"
     CDS             complement(4762..5190)
                     /locus_tag="MIW95_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY3152835.1"
                     /translation="MKPSDFQKTIQCQFDCKLKRVVKGIVRNYRKELKRRRNKEISYC
                     ELPEIVVEKLAVWDEYESDYTAFDVCGIEVRVLDDDLAEAIKYLSEKDREILLMYFFL
                     GMSDTEIGDRLKINRSTSFRSRKNSLEEIKKKLKENMNDE"
     gene            complement(5470..5694)
                     /locus_tag="MIW95_003541"
     CDS             complement(5470..5694)
                     /locus_tag="MIW95_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152836.1"
                     /translation="MIGLKKRDIKKALKAGAKAGGVSLADVRADIEATIDEAMNSTDP
                     EVQENFRKYFGNKRPTPEEYIYKITKKTKV"
     gene            complement(5726..6151)
                     /locus_tag="MIW95_003542"
     CDS             complement(5726..6151)
                     /locus_tag="MIW95_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152837.1"
                     /translation="MKKKLSFIIEIIIGIIFICFGYFVIDTDYYATLFYAMGFGLAFA
                     SGVQLLKICYYEMPKNKEKLQNINRENHINNVDERKIFLRMKAGSLVYQLMTFVYLFV
                     AFVLALLHIEAWIIGIIFGLFLLQTFLGIILYKHFEKHF"
BASE COUNT         1778 a         1279 c          898 g         2228 t
ORIGIN      
        1 aagtttaaaa atacttcata aatataatat tattaaaaaa ttctctttta aaaaactaac
       61 aatcttatat gtttacttaa acactatgtt tcttaattac aaaaatgagg ctatctcaaa
      121 ataaagataa acctcttttt cattgtttaa atgatgaaaa tatatttaat tttatacaaa
      181 aaaagtgcct catgaactaa agagttcatc ttgagacact ccataaaaac cttacataac
      241 ttgtccacca gtaatattta tagattgacc agtacagtat tcagcttcct ttgaagcata
      301 aaatgtaagc atatttgcaa catctataaa tctacaccct ctttttaaag gtactttatc
      361 tatataaact tgcttgactt ctgattcagg tattcccaat tttactgcat attgaggaat
      421 taaactttca aacatatcag aatcaagtaa attaccgagc atcagtgcat ttacagttat
      481 cttatgttct gccaaatcta gcgctaaact ttgagtaagt cctactccag caaatttacc
      541 tgctgaatat cctgaattat gcttgcttcc cactctacct gattttgaat ttatctgtat
      601 gattctaccc tcaatacctt ttgctatcat gactttagat acttctcttg caaatagaaa
      661 gtacccattt aaatttacgt ctacaataaa tttaaaatct ttgtatccaa aatcagttat
      721 ttttttactt acagccactc ctgcattgta cactaataaa tctattctat caaaatcatt
      781 ttcaatagct ttaacagtgc ttataacctg tctttcatct atggcattac aaccatatcc
      841 tacagattta caaccaaatt cagtttctat ttcttcagct actttttttg cgttgtcttc
      901 atttaaatct gcaatagcaa catcataacc tactttagct aaatgtttag ataaatattc
      961 acctaaactt cttcctcctc caacaactaa agccactttt ctcattttca tttccccttt
     1021 tttatcacat taaaagttaa cttgattagt taaattgact tttatctata atttacttat
     1081 aatatgatta gattcgtttt aaattttatt ttctaattat ttttagtata gtacctacgc
     1141 caataggtgc tatttctttt tcttcaagat ataaggtccc tccaattgct ccatcttttt
     1201 cacctgtaaa cttaaatgtt atatgaccta attctgaaag attttggttt actaactcac
     1261 caacagaagt aattttgtaa tcaacattat ttattgataa cacatcattc gtaagtattt
     1321 cttttactaa attattttca gaatgtatat aacagtaatt ttctaactct tgaggagcat
     1381 tatctttgaa agttacaaac atattatgtt gtaaaaattc atttgcatta tctcctattt
     1441 tatttatttg agtactgtat attaattcca tatcaactga acctctctct aattatttat
     1501 aaagtattct tctatttata taacccaaag cttgctaacc atgctaatag aacagttggt
     1561 gcacctgtta aaaatctaga atataaaact gacggaactc ctacttgaac tgtatcaggt
     1621 tcagcttctg caagaccaag acctactggt ataaagtcac aagctgcttg tgagtttatt
     1681 gcaaacaatg ctggtaatgc taaacttggt ggaatatttc cttttcctat ttctgtacca
     1741 accaataccc ccactatctg agctataaca gctcctggtc caagaaacgg agacaacaac
     1801 ggaaatgaac atattattga taagacaact aaacctccaa catttccgat taatggagta
     1861 agtaaattgg ctacccaatt tcctatacct gattgattta ttatacctat taggactgca
     1921 acgaaagcca taaaaggcag tatagtatgt attaatgtat ctatagtatc cctaccagct
     1981 tgaaaaagag tacttgtcac atttcctagt gctataccta tttttgcaac aattccttga
     2041 ctctgctcac ttatcttctt tttagtatca taagccatat ttcttccctc ctactacctc
     2101 aattataact gctctacatt tgttggtttt acccctgaaa catatatatc ttcttttata
     2161 aacattgcta atggtccact ttttcctgtt ttcattatat ttatagtcgg tattttcttt
     2221 tgaggatata taccacatct caatgttcca ccacaattta ttattacaca tgcgatttca
     2281 tcatctttaa caccctgtga aaatccatct attaactcac atcctgtaag ttcagctatt
     2341 tttactgcag tttctggctt tgctccccca gtcacatata ctaccttatt cttcttttct
     2401 gttggttcta ttatcaatgg tccaccccat cctccagaac cttttactat tttaactttg
     2461 ttaaatgcca tataatttat cctcctctat acactatctt tttatttgaa aaacttacta
     2521 aatttctgat tatcttttca taagttggta aatgccgata tatcccgtat tttagggctt
     2581 tatcggtatt ttctttttgg aagatacctt gcataagctg gcatttacca gcatgaaaat
     2641 gcaaccaaaa gtagtaaatg agtagtaaat ataagctccg atattaacaa gccagtcttt
     2701 ccagttccgc ttttgcagat tgaaatgtac cgtgtgcgta atagccaagt gtcatggtta
     2761 tgttagcgtg tcccatgaga tattgcaggg tgtttgggtt cattcctcta tttgccatat
     2821 tcgtacaata agtatgcctg aatgaatgtg gtgtgatgtt cggtaactta tccgtgtgat
     2881 acttgttata tttcttaatc aatcctcgca ccataccctc atagtttcct gcaactttag
     2941 gcaagccctc acggtttaag aataggaaat tgctgtaacc acctacaatc agcggttgtg
     3001 cttttcctct gctctttagt attctttgga ttgcttgata agcccgttct gtcaatggaa
     3061 gttcccgttt tccgtttttg gtctttggtg tttcaatata gtagcctatt tcgctatctt
     3121 tcaatagctg gtggtctata ttgagtattc tgttctgcat atcaatatgc gtcgtcagtc
     3181 cgcaaaattc agaaatacga agtcccgttt ccagcagaag cacaacctca tcataatact
     3241 tgctgtaaat cttgtccttt tccataaagg caagcaggcg ttcttcctgc tctggtgtaa
     3301 ggataacttt ctgctccgta tcatcttcca gaacatcact tagcttaaat tcaaacgggt
     3361 tctttctgat acagtcgtct tgtatcgcca tgtaaaatga cgctttcaag gaacgcttat
     3421 agttatcaat cgttttatag gcatatcctt tttcactcat tctgatagcc cattctttag
     3481 cgtccgattg cttcacagta tcaatcgccc tcatacccaa cgggtcgttt ttcagagcgt
     3541 tcataagata ctgtcgtcct ttttcagtag cccttttgat gttctttctt tggctgttct
     3601 tcttgtcgta gagctggcag actgtcattt taccgccgac tgtgtcgata ccgtcgttaa
     3661 ggtctttttt tatctgtgct tctttttccc tcaacgatat atcatcacgc tttccagcag
     3721 gtgtcttgtc tgtcggtaca agtttccaag aatagacaaa ttgtggcttt ccgtatatat
     3781 cggtatattt ataaacgtat cttccgtctt ttcgctggct ctctccatta cgcaaattgc
     3841 gatttttacc gtcttttcgt tttacattag acatagtgta aagctccttt ccgtcatgga
     3901 atgagccgtg atacgctata tttattatac catatctacg gctcaatgac attagatttc
     3961 gtccaatgta tctatgattt tttcaaattg ttttcgctta atctgaatac ggttaccgtt
     4021 cacgataacc cagccagcgt caatattttc ttcggctaat ttacgcaatt tcttttcgcc
     4081 aatgcggaag tatttggacg cttcttcaat cgtcagcgta tatttttccc agataggcac
     4141 atcagtattg ttcataatct atgcaccccc tttactgtgt gtcatttttt acaagcaggc
     4201 agacggcttt ggaaagctcc gttagtatct caactattcc cattcttgtg ggcagaaccg
     4261 caccatgcgg acgtatcatt attctgtgag gataggtcgt ggcaaaacga cttttccaac
     4321 agtcgctctc ggatcactgc gtgggtcgct cgctttcttt tgaaaaggtc gtggcgtaca
     4381 gtccccgtgg ctcgctatct cacaaacgta tctgtctgct ttattcagtt gtcaaagagc
     4441 tgtggcgaaa gcgtgttgct ttcatgtaaa agcaggggca gagcaggaaa gaggggattg
     4501 aacaaatcat gctctgcggt tactgcttgt tattcttcgg tttctaaagg aatatcaaag
     4561 tccaaaatca ttttgataag tgcttctctg atacgtcctt ttaattccat gtctacgact
     4621 atgcagacat taccatattt gtcgtagaac ggacgcaaac aacattttga tatgtacggg
     4681 tcatacaacg ccagtatctt ttcaatcgca tttacgttgc cgtccacagc cgacgaaata
     4741 aggtaaaatg acgggcgtgt gttattcatc attcatgttc tcctttaatt ttttcttgat
     4801 ttcttcaagt gagttcttcc tactgcggaa cgacgttgaa cggttaatct ttaatctgtc
     4861 gccgatttct gtatcgctca tgcctaagaa gaaatacatc aacaagattt ctctgtcttt
     4921 ttcagacaga tacttaatcg cttctgctaa atcatcatca aggacacgaa cctcaatacc
     4981 acatacatca aaggcggtat aatcgctttc gtattcgtcc cagactgcca gcttttctac
     5041 gacgatttct ggaagttcac aataggaaat ttccttattt cttcgtcgct ttaattcttt
     5101 gcgataatta cggacaatgc ctttcactac acgtttgagc ttgcagtcaa attgacattg
     5161 tattgtcttt tggaagtccg acggtttcat atcatcacac cccctttccg ttatgacgtg
     5221 aaaagtgttt atccctcttt ccgcaccata tctgtacagc aaggtgtgat ttgttgcatg
     5281 attttgaaaa aaaatgaaaa aattttttcg ggtacaaaaa aagcccgcac aaaacatata
     5341 gtctgtacga gctattaaag ctgtgagata ttcagttgtt aataaaatgc tgttaggtag
     5401 tgtatgaccg tataagatta tgcttaaaac tttccatgac acaagccccc ttacagcact
     5461 tgatttctat taaacttttg tcttttttgt aatcttgtaa atatattctt ctggagtagg
     5521 gcgtttattt ccaaaatact ttctgaaatt ctcctgcact tctgggtcgg tgctgttcat
     5581 agcttcatca atcgtcgctt caatatcagc tcgaacatca gctaatgaca caccgccagc
     5641 ttttgcacca gctttcaatg cttttttaat atctcgtttt ttaagtccta tcatagtatt
     5701 acctcgtcac aatttactga atgttttaga aatgtttttc aaaatgctta tatagaataa
     5761 tccctaaaaa tgtttgcagt aggaataaac caaatataat gcctataatc catgcttcaa
     5821 tatgaagtaa cgcaagtaca aaagcaacaa acaagtacac gaaagtcatt aactgatata
     5881 ctaaagaacc agctttcatt cttaaaaata tttttctttc atcaacatta ttgatgtgat
     5941 tttctctatt tatattttgt aatttttctt tattttttgg catttcataa taacaaattt
     6001 tcaataattg aacaccagaa gcaaaagcta aaccgaaccc cattgcgtaa aaaagtgtag
     6061 cgtaataatc agtatcaata acgaaatatc caaaacatat aaagattata cctataataa
     6121 tctcaatgat aaatgataat tttttcttca ttgttaattc cacctttcaa aagactacat
     6181 ttt
//
LOCUS       DAGQWR010000190         6153 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.193, whole genome shotgun sequence.
ACCESSION   DAGQWR010000190 DAGQWR010000000
VERSION     DAGQWR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.193"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            180..467
                     /locus_tag="MIW95_003543"
     CDS             180..467
                     /locus_tag="MIW95_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152838.1"
                     /translation="MKKILYALYSFIVIIANFRLKEKNYNFILLAIFFILLLIQNGVL
                     KTGYQNRATFYQDNYFNGEDKNDKIKFITIKIFIFLSLPLCFNILFNYLTS"
     gene            complement(1005..1556)
                     /locus_tag="MIW95_003544"
     CDS             complement(1005..1556)
                     /locus_tag="MIW95_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3152839.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1747..2709
                     /locus_tag="MIW95_003545"
     CDS             1747..2709
                     /locus_tag="MIW95_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBY3152840.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            3004..4848
                     /locus_tag="MIW95_003546"
     CDS             3004..4848
                     /locus_tag="MIW95_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBY3152841.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKRKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASNAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDIVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            5170..5754
                     /locus_tag="MIW95_003547"
     CDS             5170..5754
                     /locus_tag="MIW95_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBY3152842.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKDKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNVKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2413 a          600 c          932 g         2208 t
ORIGIN      
        1 atagattgaa atttatgaat attcatacta taatttaaat ataaagagat gtcctttaaa
       61 aataaaaatt aaaaaatatt taatgctact acaataggaa ctagaactgc acttaataaa
      121 tatacagaaa ttagatttgg ctcaatataa aatacaaata tagaatttag gtgtttttta
      181 tgaaaaaaat tttatatgct ttatatagtt tcattgttat tatagctaat tttagattaa
      241 aagaaaaaaa ttataacttt attttattag caatcttttt tatactgctg ttaattcaaa
      301 atggagtttt aaaaacaggt tatcaaaata gagctacatt ttatcaagat aattatttta
      361 atggagagga taaaaatgat aagataaaat ttataactat aaagattttt atatttttgt
      421 cattaccatt atgttttaat atactattca attatttaac ttcttaaatt atatttagat
      481 ataggagtgt tatgataaaa gtaagattat gtgttgttta tattaaatat aaagtgcata
      541 tagtgataaa acaaaagttt catataaatg aagaaaaaat ataaaataat ttatttaact
      601 acttaaaaat aaactaaaag ggataataaa atagctttta ggttatggta ataaaggtat
      661 ttaaaaagag tgcataagca ctccaattgt gagaaataca tacacaaatc attattacaa
      721 caatgaaaaa tgtacatata agagacgttt tgtatttgtt ttaagtgata atattttaat
      781 actaactaaa taattatcac aataataaaa aagaaaaagg agtattttgt actccatttg
      841 tcaaaaatat aaaactttta tataagctta ttataacata attgataaaa gtgtaaagct
      901 atataatgaa tttataaaga aaaataaaat aaaaaagata aatcctaatc aaaggattta
      961 tctttttgta tagaaaataa ttattctatt attaaatggc atggctaaac tggataataa
     1021 tttattatta ctgctttcaa tgttttttct ccttgattat ggtatgtatg aattgattca
     1081 gctgaaaaac aaatagagtc attttctcta agttgataaa tagagtcatt tacttctaat
     1141 tttaattgac cttctataat cataatatat tcctgtgatt tttcagaatg accaacagat
     1201 gtataactat gaccttcttc taattccatt tgaaataatt cgaagtttct agttggtgta
     1261 ttgggataat aacaataaag acgataatct ttattttctg aaacttgtac atctatatca
     1321 gtttttgata caataaaagt ttcattttga ggttgttcca gtattgcagt atatggaaca
     1381 tttagtccac ttgcaatttt ccaaattgtg tttactgttg ggtttgagtc tcccttttca
     1441 atttgcgaaa gcattacttt acttacgcca gataattcag caagttgacc tagacttaag
     1501 ttacgttcat tacgtaatct atttaaattt ttagcaataa cagtattaat actcatagta
     1561 actccttaaa atgtaaatta ttgacaaata taatttacga atgtataatt tatttaacat
     1621 tatttataat ttacaaatgt ataatataat gaacgttaaa tacactgtac atccgtaata
     1681 taaattatac tgcataaaga gttgtttttc aacggatgat agttgagaga ttaaggggga
     1741 attgtaatgc gtgtacttaa taatgttaca gaggctatag gcaatactca tatgttacat
     1801 ttatccaaaa tagcagatga atttggagtt caaggtaata tttatgcaaa gatggaatac
     1861 ttaaatccgg gatttagtaa aaaggaccgt gtagcattac aaatgattac cgaagcagaa
     1921 aatgcaggtt tattagttcc aggtcaagca gttgtggaat taacaagtgg taatactgga
     1981 acaggactag caatagcttg tgcatgtaaa ggatataaat ttatagcgtg tatgtcaaaa
     2041 ggaaattcta tggaaagagc aagaatgatg aaagctttag gtgctgaagt agttcttgta
     2101 gaccaaatgc ctaattcagt tcacggacag gtatctggtg aagatttggc acttgtagaa
     2161 attgaggctc aaaaaatagc aaaagaaaga aatgcattta gagcagacca atttaattta
     2221 gaagcttgta atcatgcaca tgaaaagtat acagcagaag aaatgtggga acaattggat
     2281 ggaaatattg atgtatttgt agattttctt ggaagtggta gtacttttgc aggatgttca
     2341 aaaacactta aaaaacataa ttcttctatc aaatgttatg ttgttgaacc tgaaacagca
     2401 gcaatttatt caggtaaaga aataactgac caagggcata aaatacaagg tggaggatat
     2461 tctatggatt tgccattagt agacaagaaa cttattgatg gttatattca agttacagac
     2521 gaggaggcaa ctgatgtagc aagaaaatta gctaaattag aaggtgtatt tgcaggattt
     2581 tcttcagggg caaatgtgtg tgcagcaatt aagttgttaa agagcagtga aaaaggaaaa
     2641 aatatagttt taactcttaa tgatagtggt ttaaaatatt taagtactga tttatatgaa
     2701 gaattgtaat aatagcacaa tagttatgca atgtttttta aattaagtat atagatttaa
     2761 attataaaaa gtatctcaat gtgatttttg aaagttataa aagagatact tttttatttt
     2821 gtaaatatgg agtatcctac ttataagtat taagctatat agatattgat gattagctat
     2881 gcaataaaaa agagtatgtg tttttgcatt tatacatcat aacttggaat aatacatatt
     2941 aatgttctaa atgtatttaa aacatatgat aaaattagaa caaacagata caaggagata
     3001 aacatgcaaa agagaatttt aagaaatttt atatttacgg gtaaaaatat tttaagtaaa
     3061 cttgttttta taataaaata taattttagt acattaattt tatttgaatt atttcataaa
     3121 ggattagcct tattttttat atggccatct attagatata tatttgatat tttaataaaa
     3181 agtttacata tagtctacct aaatatggat aattttataa aagttttgac caatcccatg
     3241 tcaatagttc tgataatact atcagttata atactagctt tttatgcatt ttttgaattt
     3301 acctctgtaa ttatttgttt taataaatcg ataaaatatg aaaaaatagg gttatttgaa
     3361 ctctttaaaa tatcatttaa gaactctata aaattgttat atcctaaaaa tatactatta
     3421 tttatatttg tgatattaat aattccatta gtaaatacag ttttaatttc agggtttata
     3481 ggaaaaatac aaataccaga gtacatatta gattatataa agtctgatat aattttaaat
     3541 ataatctata tgatttttct tttaataatc tatatttcgg ttattagatg gatttttagc
     3601 attcatgaga taactttaaa tacagaaaat tttaaaagag caagaaaaga aagtgttaat
     3661 cttataaaga gaaagattat aagaacaatt atttattcat taatattatc tttagtagta
     3721 gctattattg gatatgcgat ttatcatagt ggaattatat ttataggcat atggactaaa
     3781 tattgtactg atatggaatt tttgaagaat gtatttataa atagatgtat tgcatttgaa
     3841 gaatatttta gattgatttt atcaatattc atttttatat tggacattgg atttatctca
     3901 gcaacttatt atcaatataa aggagtaaat atttctaaaa ataatattaa aaaagagaaa
     3961 aaatctatat caaaaattgt ttttaattta gtattaatat tattgatttc ttatatagag
     4021 tcagttgcat tttcttcaca tataaatggc atgtttaata cgtcattttc attttataca
     4081 acagcaattg ctcatagagc aggagctgaa atggctccag agaatacttt ggcagcaata
     4141 gaagaagctt caaatgctaa agctgattat gcagaaatag atgttcaaga aactaaagat
     4201 ggagaactta ttgtacttca tgattcaaac tttaagagaa ctacaggagt agataaaaat
     4261 gtttgggatg taaattataa tgaagtaaag acatatgatg cgggaagctt ttattattat
     4321 ggaaaaggtt attatgatga aaagtttgtt ggggaaaaga taccaacact caaagaaatg
     4381 ataaaatatt ctaaaggtaa ggtgaaatta ttaatagaaa taaaacttaa tggacatgaa
     4441 aagggagaca tagtaaaaaa agttgttcag ttaattaaag aaaataattt tgaaaatcaa
     4501 tgtatagttg catctatgga taaaactata ttacaaaaag taaaaaaact ggatgagaat
     4561 ttactgacat gctattttat ggcaattgca tatggtgatt tttcaaagtt aaattatgtt
     4621 gatatatatg gaattgaatc aacttttgtt aataaaacag tagtagaaaa gattcataaa
     4681 atgaacaaaa aaatcttcgt ttggactgtt aatagtgaca gtttaattga gaaaatgctt
     4741 gacctaaatg tagatagtat tattacagat aatccatacc ttatagagag tgcaatttat
     4801 tggaagaaaa atggatttat tagtcaagtg tcagattatt tattttaaat attctataat
     4861 tgtattatcc agtaaaagag atattattaa aatcaataat atctcttttt aacataaata
     4921 aggttgttaa attaaggtta ttcttataaa aattgtgctt atcagctata aacaaggaat
     4981 aaaagtgaaa aaattgctct gagattatta cagttaaaag agttttaaga tgacagagag
     5041 atagattgtc aactgtatat ttaataaatc ttgttattta ttaaatttta tataagtaaa
     5101 atatataaat taataaatct tatgctagaa tatatatgaa aaacaatata aatttggagg
     5161 attaaaaaca tgacagagcg agaaaaaatg ttggcaggag aactttacga ttgtggtgat
     5221 attgagttgt taactcaatg gcataaagca aaagacctag taagagatta taaccaaaca
     5281 gattcctcgg atttggaaaa aaaagaaaaa atattaaatg aattacttgg tggtaaggat
     5341 aaaaacttat ggattactgc accgtattat gtagattatg gaaataatat ttattttgga
     5401 aataattgtg aagtcaatat gaattgtacg tttttagatg ataataaaat tatcattggg
     5461 gataatgcac taatcgcacc aaacgtacaa atatatactg catttcatcc aactaatgct
     5521 ggtgatagat ttggtaatgt caaagaagat ggttcatttg aattttgtaa aactcaaaca
     5581 gctccagtta ctattggaga taatgtttgg attggcgggg gtgctattat catgccaggt
     5641 gttactattg gagataatgt agttataggt gcaggaagta ttgttacaaa agatattcca
     5701 agtaatatga ttgcatacgg aaatccatgc cgtattattc gagaaaacaa ataaagttaa
     5761 attttcttgc aaaatctcaa aaaacacctt tcttacttta tacatagggt attttccaat
     5821 actttcacat ccatattcat ttgttaaatt tttgtggaat gtagttttta tcacttctat
     5881 cttacatttc caccattttt tataaaagtc attcctttat agatttattt ataatagtat
     5941 atattctatt tgatgtttga ataagcactt atttttaagt aaaatactaa cttttaggtt
     6001 agtattttgc tatagtatga tgattaagta agaataaaat actataatac ataaagctta
     6061 ataaatataa tttttatttt acttagatat tttcttttga ttagtaatca aaagtggtta
     6121 taattttttt aattaataag agtaatattt att
//
LOCUS       DAGQWR010000191         6027 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.194, whole genome shotgun sequence.
ACCESSION   DAGQWR010000191 DAGQWR010000000
VERSION     DAGQWR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.194"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(214..723)
                     /locus_tag="MIW95_003548"
     CDS             complement(214..723)
                     /locus_tag="MIW95_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBY3152843.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(725..1372)
                     /locus_tag="MIW95_003549"
     CDS             complement(725..1372)
                     /locus_tag="MIW95_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152844.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(1386..2558)
                     /locus_tag="MIW95_003550"
     CDS             complement(1386..2558)
                     /locus_tag="MIW95_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY3152845.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAE
                     RLKLEFEKEGLDCELVDVGENGNTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGALCAFNMETGLVDNSLCIGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSRENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            2826..4202
                     /locus_tag="MIW95_003551"
     CDS             2826..4202
                     /locus_tag="MIW95_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152846.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEILNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(4453..5286)
                     /locus_tag="MIW95_003552"
     CDS             complement(4453..5286)
                     /locus_tag="MIW95_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY3152847.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     EDIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILVFIVLLIIGVYKLFKKY
                     N"
     gene            complement(5615..>6027)
                     /locus_tag="MIW95_003553"
     CDS             complement(5615..>6027)
                     /locus_tag="MIW95_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBY3152848.1"
                     /translation="GIDYENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMG
                     ASKKGSYGASIIEKIESIIPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQ
                     QLNNHEESQSHPDEQEIYEASMFAKNMMIKASIV"
BASE COUNT         2102 a         1028 c          646 g         2251 t
ORIGIN      
        1 tttcattgtt gaaattatga aaatatactt gatttaatac caaaagagta tctcatgaac
       61 taaaaagttc attttgagat actctcttct tttttaatta atatacatat taattactat
      121 attagtttat tactatatat caattactat tcattaatta ctattcatca attcttatat
      181 atcatttata attaggttat aattttatgt tatttacatt aatcccataa cagtgagtaa
      241 aactcttact atcaacatcc ccactattgc atcaaatata ggaactgcca acagtaacat
      301 tctatactta gtactggcat ttgctacaag cactattctg gcataatgac caactagagt
      361 acccattaac atacatggca taattaatat ggttgcttgt ggtgcagtga ttagaccttg
      421 tgcaaataag tttgcagctg tagctgctcc tgctgcctta gcaaagaatg ctgctatcaa
      481 aactacaaca gattccccag gtagtccaaa aatacccatt actggaccaa aaatatttcc
      541 taaaatatca actaaaccag ttaattgtaa aaactgcact attacataac ccattatcat
      601 ggctggtaat atctgttcaa cacctatgta aaaacctttt tttgctcccc ccataaatat
      661 ctcaacagca gttttcttag gtttactatt tacaattgca tttccagtat tttgttgact
      721 catactacac agcctcctta gccttattct ttttatttct tacacctatg attaatctaa
      781 ctagattagc accaagtatt ttacagaata tctctataag gattattgga cctaatgcca
      841 ataatgctat tggtaaaagt ggagcttgag tatttattgt gttcagtata acagctgaac
      901 cagcatattg atatgctaca aatatagttc tttcatcatc tgttatttcc ccactttcaa
      961 ataattcttt cgtcattatt gaggctacat ctgaactagt aaatgaagat acaaatgcta
     1021 ttcctgctat tccaggtata ccaagcagag gtctaagtaa tggattaaat atctttgatg
     1081 ctgctttcat tcctcccata gattcaacta catctataag acctacagca aaacagacag
     1141 ttggaatcaa agtcagagca aataagaaac catcttttgc tcccgttcct cctttaccca
     1201 taaagtctac accatctgca atagtaccaa atgaacctgt cagattagca aaatctaatg
     1261 ctttaaggaa gccttgacta tctttaaata ctcctgaaaa taatactatc aatacaaata
     1321 aagataaata ccctttccat gttaccttat tatctccagc tgttacatcc atataaaatc
     1381 cccctttata cctcaaattt atctaagttt aaaataactg tagaaattaa tttcaccctc
     1441 tcaaacatag aatctactac tgcatattct cttgatgtgt ggttccattc tcctcttaca
     1501 ccaaatgaac agattgtagg aacgtttgca atagttaaat aacttgcatc tgaactacct
     1561 ccaagtctct tagcatttaa ttctccaaat ccattttctc ttgacacttc atttacaaat
     1621 gtatgaaatc tcataacatc ttctgtagtt tcaaatggca tcatttcact tataaaatta
     1681 acttctgtag aagttccttc tatgtaagtt tctttacata tctctctaac ttgttcttta
     1741 acattctcca tttcttctac tttttcaaat ctaaggtcta cttttatact acaatcttct
     1801 ggaatagaat tttccactct tccaccctta atcacactta cacttactgt agttccaaca
     1861 tctaaattag tcaatttttg tatgcgtagt atcttattag ccatttcttc tatagcattt
     1921 cttccacctt caaaatcatt acctgcatgt gtttctacac cttttacatg tatattacat
     1981 cctattcttc cttttctacc tatacataaa ctattatcta ctaatccagt ttccatatta
     2041 aaagcacaaa gagcaccttt tgcttccctc aatataacat ctgcaccagt tgaatcttta
     2101 tgacctatct cttcatctcc agaaaatact atttttattg gtctttcttt gtatcctatt
     2161 ttatttaaag ctttaataac gtataaagag attattatac cccccttcat atctaaaaca
     2221 cctgggccat atgcttttcc ttctattatt ttaaatggat tctctccaaa tgtacctgtt
     2281 tcaaagacag tatccatgtg tcctgaaaaa attatcggct ttttatctat attagaacca
     2341 agtgtaccta tcaatgtatt accattttcc ccaacatcta ctagttcaca atccaaacct
     2401 tctttttcaa attcaagctt taatctctca gctaacttgt ttacactttc tttacaatgt
     2461 gtataacttt ctatattaac aatctctttc cataatgaaa ctatttcttc tctattctca
     2521 tcaacaaact tagaaatttc atttaaatat gtattcataa tcttctcctt tattttcaaa
     2581 taaaatgtat ttctttccta aaatctaagt ttattttagg aaatcttaaa agactagtcc
     2641 ttaattacaa aatagcacaa ttattcttgc cagtcaacat atttttcagt cttttttata
     2701 ttttctgatt tttctttcaa ttatcctttt gtaaaataaa ttgacgcgtt ttatctacta
     2761 ttataaaatg aggttataaa ttgattgatt agttacaata ctctcgaaat tttaaaggag
     2821 gttttatgaa atataaaatt ggttttttga caacatctta ttttatgtca gataagttta
     2881 aacaagctct taaagaatta aatgatactt gtgaaattac atttattgag cttaaagggg
     2941 agaatccttt taataattta cctaatatat actatgagca agtagataat tttgatggat
     3001 ttgtatgcag tggtatagtt ccatatagtg aacttataat aaatataaaa gacataaaag
     3061 ttccattaaa ttttttaaag cttgatgaaa gagattttta taaatactta ttcaaattat
     3121 taaatagcca aaaagatatt gactttacaa aatcatttat ggatttttta agagaggaca
     3181 acaattatta tgatatatat tcattaatag actctaataa atgtccatat acaattaaag
     3241 attttaatat ccctcaacct gtatatgact ttaaaaaatt aaatgataaa ttattggaaa
     3301 tacatttaga cttattgcga gataataaga ttaacctttc ttttactaga aattacatca
     3361 ttagttgtga acttaataag cttggatatg attgtattta catggttcca tctacagaat
     3421 caatcctgaa tacatttaaa tctctggtga atgaaataac attacaaaac ttagacaaaa
     3481 ataaatctgc aacttgtata gttacagtaa atagttcaga gtacataaat gaagatttga
     3541 tatttaaaaa tgatgagata caaaatcaaa tatataacac tatattaaat tctctatcaa
     3601 gacatggatt ctatggagtt caggtcaaaa aaaatgatat gaaaatagag atacacacta
     3661 ctaaagaaat attaaacaat atgaccaata actatacaga tttttttatt tcagaagatt
     3721 taaaagaaat taaatatagc ttaaatatag gttggggcat tggaaataca aatattcatg
     3781 cagagcaaaa tgctcgtcag gctaatggta aatctgcttc tttgaatgga aactgtactt
     3841 ttatagtaaa tgatactaat gatacaatag gccctttata ttcaaataaa aattctaata
     3901 atattgaaga tagctcaata gccaataaag ttgcttcttt tatacctcta tctaatacaa
     3961 atgtatctaa aatcatgtgt atgattaatg atagaaattc aaataatgtg tcagcagaaa
     4021 tattagcaga ctatatgaac ataactctta gaagtgctaa tagaatattg agtatcttat
     4081 ataaagcagg aattgcaact attgtaaata caaaactcga taatcaaaga ggaaggccaa
     4141 aaaaaatata taaagttgat ttcttatctt ttctaaataa gatgcagaaa ttaaattctt
     4201 aatatttaat tttttaataa aagcaatgta ttttctctaa ttttaaagaa tcaaaatgtt
     4261 taaattttat tagaatatac aactttttac tttaaaatac ctaaaccttt tatcagtgaa
     4321 ataaaccata atatatcgta gcttattaga aaaacaaaat aatttcatat acaaataata
     4381 aaatagccac tacaatccat aatttgcagt ggctactttt tacactatca cttattactt
     4441 aatttttaaa aattaattgt attttttaaa taatttatac acacctatta tcagtaaaac
     4501 aataaataca agtatatcta taaaaataat tattgtaaca tgtctggcaa tattgtaaat
     4561 aaaaagtata gagaatattt ctatagacaa cgctacaaaa cacataaaat tcgttaaata
     4621 atctgcaata gtatcactat aaaaagcatg tatttgtaac catctccata taaatggacg
     4681 taaaaggatt ctgaatacaa cacaaatgac agggtaggca taaataaatt ttttatctac
     4741 ataattagct acaccattat tccattggat aggaatttga ggtggcaact ttggaaatac
     4801 tgttattgca atcaacaaac aagcaatagt aataattccc caggtaattt tccctcccca
     4861 tatatataag aaacttacag agtcaagttt aaaaacaggt ttgtttttat tccagtattc
     4921 tggcaactca gttatgtatt gctctacttt caaattcaaa taacttagct catcagattc
     4981 taacatttta ttacacatag tttttgcatt ttctaagtct gatattttag tttcgagaac
     5041 tttctcctgt ttctctacaa cttcaactaa agatactttc tgtaatataa tattacggat
     5101 atcatcaata gtaattccta gtgtacgtaa atatgcaatt tttttgatgt cctctacatt
     5161 ttcatctgaa tattctctat agccattacg ctcattacga aggggtttaa ttaaattctc
     5221 gttctcataa aaacgaatat tagagcgtgt caatcctgtt ttttcttcaa cttctttaat
     5281 tgtcatttta aacactacct ctctattcta ttaatttaca actataatat agtcttaaat
     5341 attttcactc tataaaattg tatatttagt atatcttaaa atcaagttaa ttatctttta
     5401 taaattttca tacaataata tctcctttca ataatatctc tttttctata tttaaaacta
     5461 tcttattttc tataaaataa tgtataaaca aggtttacag tcaagtattt ttattatttt
     5521 tttgatttca taaaaaaaag catagcaaat catttgctat acttttttac taaattattt
     5581 attctatatc ttatatgaac ttattgttta actactacac aatactagct ttaatcatca
     5641 tattttttgc aaacatactg gcttcatata tttcttgttc atctggatga ctttgagatt
     5701 cctcatggtt atttaattgt tggcgaatat gttcgttgtc aggagaaagt tttaacatct
     5761 ctttatattt tgctttcaat ccttctgcta tcttaccttg acatatgaat gaacctaata
     5821 tttcattatc ttttgggata attgattcta tcttttctat tatactagct ccataacttc
     5881 cttttttact tgctcccatt gttccaaata gtgctatctt tttattgtga atattttcaa
     5941 gcacttgttt tgaattttta tcacaaattc ccttgtccac ccaatagcct aagaatacta
     6001 tatcataatt ttcatagtca ataccat
//
LOCUS       DAGQWR010000192         5983 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.195, whole genome shotgun sequence.
ACCESSION   DAGQWR010000192 DAGQWR010000000
VERSION     DAGQWR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.195"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            214..1389
                     /locus_tag="MIW95_003554"
     CDS             214..1389
                     /locus_tag="MIW95_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY3152849.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQAYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            1424..2962
                     /locus_tag="MIW95_003555"
     CDS             1424..2962
                     /locus_tag="MIW95_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBY3152850.1"
                     /translation="MQGALKENKKKSGGGFLGWIERVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQVI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            2994..4196
                     /locus_tag="MIW95_003556"
     CDS             2994..4196
                     /locus_tag="MIW95_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY3152851.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFIQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     SEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     IWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(4375..4974)
                     /locus_tag="MIW95_003557"
     CDS             complement(4375..4974)
                     /locus_tag="MIW95_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY3152852.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            5143..>5983
                     /locus_tag="MIW95_003558"
     CDS             5143..>5983
                     /locus_tag="MIW95_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152853.1"
                     /translation="MSNIITNLIESVFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVK"
BASE COUNT         2240 a          724 c         1057 g         1962 t
ORIGIN      
        1 ataaaaaaac tcattatatt aagatgaaaa tagaaaaata actagaaaaa tactataata
       61 tatcagctta atacagaaaa aagttaaaaa tattgagaaa aataaaaatg aaaacgtttt
      121 atattgacat agagaaaaag tagtgatacc atttaaatat agtttagtag ctaaactata
      181 tttaaattat aataactatt ttagggggat gttatgaatt taaaacaatt aagtgaaaaa
      241 tacgaagaat atgtaattca aatgagaaga gaatttcata agtatccaga attatctttt
      301 aaagaaataa gaacaaccca aagaataagg gaagaattac aaaaaatagg ggtccctttt
      361 gtatctattg aaccaaatat cgtaatagct actatacaag gcaaaaatga aggtaagaca
      421 attgcaatta gaggagatat agatgcgctt ccaatgcaag aagattcaga tgttgaatac
      481 aaatcagaat atgatggaat aatgcattct tgtggacatg atgctcatgg agctatgtta
      541 ctaggacttg cacatatgct aaatgatata agaaaagatt taaaaggtag agtattatta
      601 gtttttcaag cagctgaaga agttggtggc ggacacaagg agataataca gtactttaag
      661 gaaaatggag gaccagatag attaatagct actcatgttt ggtccgatat tgaagctggt
      721 aaaatttctg ttgaaccagg acctcgtatg gctggtgggt ctggttggag aatagacata
      781 acaggtagag gaggtcatgg ttctagacca gatcaaagca tagatcctat aaagccagct
      841 tgtgatatag ttttaaaagt atcttctata ccagttaatc atataagtgt actagaacaa
      901 tgtgttgtac atgcatgtgc aatacatgga ggaactacaa taggaaatgt tattccaaac
      961 aatgcagagg taataggtgg atatagatat tttactgaag aatcaggcaa aaaagtgttt
     1021 gacattataa agcaaatgag taatggtgtt ggacaagctt atggagtgga tgtgaaggtt
     1081 actcatactg gaggcatagg acctgttatc aatgatgaag aagcagttaa gatggctaaa
     1141 gaaatagttt ctaatataga tggattggag ttagattctt atgagccaat atgtgcatca
     1201 gattgttatg gtgaaatatt aaaagagtat tctggttttt attgttatct aggggtaggt
     1261 aacaaagaaa aaggaataat atatgcacaa caccatccta agtacaatat agatgaagat
     1321 acattaaagt taggttgcga atttatggct aaatatgcag ttgaattttt aaataaaaat
     1381 aataactaaa ataaatcgaa ttttataaat gggggggatg aaaatgcaag gtgcattaaa
     1441 ggaaaataag aaaaaaagtg gtggaggatt tttaggatgg attgaaagag ttgggaataa
     1501 aatgccacat cctctagcat tatttacata tattacattg atagtagtag cattatctgc
     1561 aatagcaagc tttttaggtt tttcagcaac aagtcctgct acaggaaagt taataaaagt
     1621 tatgaactta ataagtgcaa aaggtttagt actatttatg caagagtttg ttaagaactt
     1681 ccaaaacttc cctgtgcttg gggtagtttt agttatggca gttgccacag gagtttgtga
     1741 aaagacagga tttttttcaa ctgccataaa aatgagttta tcaaaagtta aggggaatgc
     1801 ggttgtattt ataatagctt ttataggagt atttgctaat caagctggtg atgctgcttt
     1861 tgtattagta ccaactcttg caggagcaat attttatgga ttaaatagaa atccattagc
     1921 tggtatattc tgtggatttg catcagttgg tggtggattt gcaacagctg taattcctgg
     1981 gggatgggac gttacgctaa cgccaataac tgtatcagct gctcaaacta ttcaacctgc
     2041 atttgatatg acattattag ctagttatta tgcactattt gtatctgcgt ttatagtagc
     2101 tacagtatca acaatagtaa cggttaaatt tatagaacct aaattaggta agtatactgg
     2161 taaaccagaa ggtatagatg ataatcaagg ttttgaggtc acaaaagaac aagcaaaagc
     2221 agcaaagtta gcaggtttta cagttttagc ttatgtagcg ttattaattg ctttgtgtat
     2281 accagctaat agttttttaa gaagtccaga aggttcttta atatttggag caccacttat
     2341 gtcttgctta cagtttttta tagtaatact attcttcttg ccagggattg tgtatggaat
     2401 gagggttggt aaaatcaaga ctgtaaaaga cttaaatgat atattatgtc aatcaatggc
     2461 agcaatggca ccgtttatcg tacttgcaat agttataggt caattcttaa cgctattctc
     2521 agtgtctaat ttagcacagg tactagctat aaaaggtgga gcattactca actcattgcc
     2581 actaccacca caagttataa tactgctatt cttagtacta gtagcgttta taaatatatt
     2641 tgttataagc ggaagtacaa aatggatgat atttggacca gtatttgttc cgatgttaat
     2701 gcaattaaac atacatccag catttactca gtttgtatat agattaggag attctgtaac
     2761 taatcattta tcaccattaa atgcattctt tataatactt ttagcaacag tccaaaaata
     2821 cgataaaaat gcaggtatgg gaactatatt ctcagctatg ataccttaca ctataggtta
     2881 ctttgtagta cttgcagttt tagtagtagt ttggatgaca ataggtatac cagtaggaat
     2941 aggtggacaa gtttggttat aaaacatatt taataatttt gggaggaaag aaaatggaaa
     3001 caactgaaat aagatacaat tttgatgaag taatagatag aagtggaaca aactgtctta
     3061 aatatgatct taagagtcaa tttatgccag aatctccaga agatgcacta ccactatggg
     3121 tagcagacat ggattttgca tgtgctcctg agataattaa tgcaatgcat gaaagaatag
     3181 acagaaaaat atttggatat agctctcaac aaactgatga ttattatgaa gctgtatgtg
     3241 gatggtttaa gagaagattt gattggaaaa tagaaaaaga gaatatattc tttagcccag
     3301 gggttgtgcc tgcgttagga tttttcatac aaatattaac acaaaagggt gatggtatca
     3361 taatagaaaa gcctgtatac tatccttttg aagataaaat aactaataat gaaagaaaag
     3421 tagttaatag tcctttaaag tttgaagatg gtcattattc tatggattat gatggatttg
     3481 aagaattagc taaagatgaa aataataagg tgtttatatt atgtagtcct cataatccag
     3541 ttggtagagt atggaaaaaa gaagaactta gaaaaatagt agacatatgt aagaaatatg
     3601 atttgtggat aatatctgat gaaatacatt gtgatattat tcgtaaaagt gaaaagcata
     3661 taccattaga agtagcatgt cctgattata aagatagaat aataacttgt acagctccaa
     3721 gtaagagttt taacttagct ggtatgcaat tatcaaatat agtaatcaat aataaggaat
     3781 tacaagaaaa atggatgctt gaaacagatt ctaaattagc tatattccta cctaatccat
     3841 tcgcaatagt tgctactaaa gttgcttata atgattgtga agattggatg aatcaagtaa
     3901 atgagtatat agatggtaat attaaatatg tagaagattt tgttaatgag tatatgccta
     3961 aagtaaagat aataaaatct caaggaactt acttaatttg gttagacttt agagggtatg
     4021 gattagatgc aaaacagctt gaatatgtaa tgcttaacaa ggcgaaggta ttgctagatg
     4081 aaggatacat attcggagaa gaaggagcag gatttgaaag aataaatgtt gcttcgccta
     4141 gaacaattat agaagagtgt atgaatagaa taaaatgtgc atttgaagga ttataatagt
     4201 aatatctaaa taatatattt gtgtatttta aaaaaagact atgttaaaaa aatatttcaa
     4261 agcatagtct tttaaagttt tatgatgagt ttaaaaaatt tcaaaacgtt actattattg
     4321 ataatcttca cttatatgtt tatattagaa ataattccac aatatgctca ttttctaaag
     4381 ttgcactttt atacaccttg cttcaccaat atcatgtgta accaaaatag tagacttatt
     4441 ctcattctta ataatactat aaacatcatc acaaactaat aatctagtct gataatcaag
     4501 agctgaaaaa ggttcatcta aaagtagaat atctggattt acagaaagtg ttctaataag
     4561 cgcaactctt tgtctcatac ctccagataa ttcttttgga tacatacttc taaaatccca
     4621 taatccataa gtctttaaaa ggctttcaac tctatctaaa gattttttat cttttttgcc
     4681 ttgaatttcc aatccaatag ttatattatc cattatattt ctccattcaa gcaaattatc
     4741 tttttgaaac atatatccaa tatttccagt aatctttata tcaccactag tcggttttaa
     4801 aagattagta agaatattta atattgtaga tttaccactt ccagaaggac caagcaaagt
     4861 taatatttct ccttctttca agttaaaatt gacatccttt aatacttcaa gctcgccttc
     4921 cttagtgtag aagttcatat ttatattttt aacttctact atgttgtcat tcattatgcc
     4981 accccctata acttttagct cgtatatatt atgttattaa caatatgacc tatttgtacc
     5041 atttttcata aatataatat aaatattttg tttatttacg tattttctta gtaacttgta
     5101 cgtataaatt ataataaata aattataagg taggggataa atttgagtaa cataataacg
     5161 aatttaatag agtcagtatt tactaatcca ataactatat tgtttttact tgcaataact
     5221 gtgtattctg tttttaaaat aatagaaaat aacagagtat attcatttgt aagttcaaaa
     5281 ttagacgaaa tcaatagaga ttataagaca agtgagtttt ataaaaaggt aagggatgac
     5341 tactatacat atagtaaaga aaatccttat gcagacgtaa atataacttc atttatagag
     5401 gaagttgtat ctgatttaaa gcataataat ttgccattgt tagaaaaaat tagaagtata
     5461 aaaaattcat cttcaataag tatattactt ggggttttag gtacgtttgt tggtctttct
     5521 acaatgcttt tatgtgttga tactaaagac attataaatt cacttccttc aactattagt
     5581 agtatgcaaa ctgcatttac aactagtata tttggagttg tattttctct aattataggt
     5641 tactttacta agataaaaga ttgtgaacat gttttgatac aaataatgtt aaaatctgaa
     5701 aatttactaa catctgaaat aactcatttt aagtcagaaa gaatggattt aaaagtagaa
     5761 gaggtaaaaa atacaataaa acaaataagt aagtctatag aagctattga aaaatttgat
     5821 aaaatatcta aagatttaaa tgactttaat gatgaattta tcagtggtat agaagcttta
     5881 aaaagccttt tagaaggttc tcaaagttct attaagacat ttgaccagag cgtaagaaaa
     5941 ttagataaac agtttaatat tttaaatata aaatttgtaa agc
//
LOCUS       DAGQWR010000193         5980 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.196, whole genome shotgun sequence.
ACCESSION   DAGQWR010000193 DAGQWR010000000
VERSION     DAGQWR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.196"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..477
                     /locus_tag="MIW95_003559"
     CDS             <1..477
                     /locus_tag="MIW95_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY3152854.1"
                     /translation="GLIISDNQFDYKNFLNYILLPIISLVYIYPFLCEEYGWRSFLQS
                     ILFDRFGKKIGIVMVGTCWSLWHLPLQFTLYSPQTPIIGAIAHLIYGIGLSIFLGYVY
                     MKTKNIWFCSIMHVLINSLGLIFNDSEIIINYHSIIQRLIFILLFYTPFLFTKEYK"
     gene            857..2383
                     /locus_tag="MIW95_003560"
     CDS             857..2383
                     /locus_tag="MIW95_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBY3152855.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NNGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            2411..2968
                     /locus_tag="MIW95_003561"
     CDS             2411..2968
                     /locus_tag="MIW95_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152856.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            3490..4533
                     /locus_tag="MIW95_003562"
     CDS             3490..4533
                     /locus_tag="MIW95_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152857.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKG
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            4644..5786
                     /locus_tag="MIW95_003563"
     CDS             4644..5786
                     /locus_tag="MIW95_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBY3152858.1"
                     /translation="MSEEKNPKENTLDEPKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2494 a          497 c         1020 g         1969 t
ORIGIN      
        1 ggattaataa taagtgataa tcaatttgat tataagaatt tcttgaatta tattcttctt
       61 cctataatat cattagtata tatttatcct tttttatgtg aagagtatgg ttggagatct
      121 tttttacaaa gtattttatt tgatagattt ggcaaaaaaa taggtattgt aatggtaggc
      181 acttgttgga gtttatggca cttaccttta caatttacac tatatagtcc tcaaactcca
      241 ataattggag ctatagccca tttgatatat ggtattggac tttctatatt tttaggttat
      301 gtttatatga aaactaagaa catatggttt tgctctataa tgcatgttct aattaatagc
      361 ttaggattaa ttttcaatga ttctgaaata attattaatt accatagtat tatacaaaga
      421 ttgatattta tactattatt ttatactcct tttctattta caaaagaata taagtaagaa
      481 ttacaattaa tttatataaa ataaattttt aagtaaatat ttttatagag agattgttat
      541 attacatatc agaaaaatat taaacgaact aaatcaataa aaaagttggt ggctaacaat
      601 ttataattgt tagcacaata taatcttatg tattgaaatc tagaatatcc tcatttttac
      661 ttagcataaa tactattgat taagaaaata atatagaaat tataaatata tataagttta
      721 gtctggtgta taaaacaaga aaactaagtg atttttcatt tagcttttta ttaagcttat
      781 tttttatact atttataata aataaactcc atgatgggaa aactattact atttaatatg
      841 taaaggagta ttttaaatga atgaatcata ttggttttta aactcaagtc caaaagaata
      901 taacaaatta ggtgaaaata taaaaacaga ttgtcttata gtaggtggtg gaataactgg
      961 attgacaact gcatatttat tagcaaaaga aggtaaaaaa gtagtgcttg ttgaggctga
     1021 taaaatagga tatggaactt ctggaaggaa tactggtaag gtaacttgtc agcatgatat
     1081 attttactct aagatagaga aaaaatatgg attagataaa gcaaaatcat attataatgc
     1141 aaataatgaa gctttaaatt tagttgaaca aataatagaa gaaaacaata taaaatgtga
     1201 ttttaaaagg gaaacttctt ttgtatttac agaaaaagag gatactataa agaatattaa
     1261 agatgaatat cgtacatgta aaaaaatagg aataaattgt gagtatcatg aaactataga
     1321 aaatattcca ttagatataa agggggctat tagttttact aatcaaggtc aatttaaccc
     1381 aaagaagtat attgatggat tggcaaaagc agcagtaaat ttagggctaa aaatatatga
     1441 aaatacacca gtagtagatc ttgaaaaagg aaaaatatgt agggttaaaa caagagaaga
     1501 taatataata gaagctgaga atgttataat ttcatctcat agtccatggt atgatggatt
     1561 gaatttttat tttgcaaaag aatatgcaga gcgtgcttat ttaatggttg cagttttgga
     1621 aaataaatta gcagatggta tgtttataag tatagatgac ccatcaataa cttttagaca
     1681 atataataat ggaagtgaaa atttactgat ttttggagga ggagaccata aagttggcca
     1741 gggaggaact gagaaagaaa tatttgatga tttagagcat tatggtaaag aagtctttaa
     1801 ggtaaaagat tttaagggga aatggtctgc tcaagataat atgagttttg acaatgttcc
     1861 ttatattggt tatatcaaca aaagagaaga caatatttat gtagctacag gattctctaa
     1921 atggggaatt actaatggaa ctgctgctgg aataataata aaagatttga taataaataa
     1981 taattctgat tataaagata cttttaatcc atccagatta ggtagctact tttcaaaaga
     2041 ttttataaag gaaaatgcaa acgtagcaat aaattatgta agtggaaaat taaaaatagg
     2101 tagtggagat atgcctaaaa ataatggaga aggaaagatt gttaatattg atggaaaaag
     2161 atatggtgta tataaggatg ataatggaga tttttatatt gtagacacga catgtactca
     2221 tttaggatgt gaacttaatt ttaacagtga agaaaaaaca tgggactgtc catgtcatgg
     2281 ttctagattt gactataagg gaaatatact tgaaggacca gctcttaagc cattaaaact
     2341 ttatggacat ggtgataatg atgttaatcc taagttatta taaaattatt tttaggaagg
     2401 taaagtgtat atgaataaac ttttaaagcc aataactata ctattgtata tagtttatat
     2461 tttatctgcc gtattttttg tagcaaacaa agaatatgga agtctaggtc tagtagcaat
     2521 tagtttagtt ggtactttca ttttgagttt tataaataag caaactgaaa gattattgag
     2581 taaagaatta tatataatgc ttgctttatt tataatgttt tcatcattgt taggtacttg
     2641 ttttaatttt tatgatataa attattatga tgattttttg catttatggt caggtataat
     2701 atcttgtatt gtggcttttt ctataattaa atacttttat acacaaagag agataagtaa
     2761 aatgagtaaa gtgtttttag ttatattttt atttatgttt agtatgggag tagcgagttt
     2821 atgggaaata ggtgaatttt taatggatac ttttgttggg actactacac aggccggggg
     2881 gttagaagat actatgattg atatggtaga tgctttaata ggcacaatta taactattcc
     2941 ttttatagta aaaaaatcta atatatagtt ttaaattttt tatattaatt ataatggatt
     3001 taattaatca tgttgagttt caaggtctat atgattaatt aaatccattt ttgttattta
     3061 taattattat tgaatgtatt tttacatata agatataagg tgatagatag aaattgttgg
     3121 attatgtaaa taattaatat ataataccat tatgggcaat aaatacaaat caaatgataa
     3181 tatagtctat ttatgtaaat gttatattgt atcctaagta taatagtaag ttttttgata
     3241 gtggtgtaga aagtaaaaat ctactagcct aaattgatgt atggattagt ttaggaacag
     3301 taattgcata tcttaagttt gatgtcatgt tgggctacta gaaataggtc atctttttca
     3361 cttgaatatc ttttctttaa acatgaggag tatcatatca aaaacatatg aaaattaagt
     3421 gatttataag aacagtaata ttttatatga tagtatagaa tagtaataaa gtattaggag
     3481 gaacattgag tgttaaaaaa gtggtttggt attgtgaaaa aaagacaaaa aagtgagtca
     3541 gtaaaagaag aaggtgaagt aatattaaaa aacgaaaaaa tattatctga agaaaagttg
     3601 atagatgaag aaggagttgt agttaatatt gataatgaaa tattaactaa agtagaagta
     3661 gtaaatgatg ataatgaaat agaagaaaaa ataatagaag aagattggtt aattagtgaa
     3721 aataccataa aattagatga taaagaagca attattaatg ataaaaacat agaattatgt
     3781 aaaaaagaag ttcaagttga aagtgaaaaa ataggtttaa ataagtttga aggacttgac
     3841 caaaatgaaa attataatct agaaaaaaat gttattgaag aaaaaaaagt aagtgaatgt
     3901 ttgacagaag aagatttaga gtacataaaa gaaattaaaa taaaaagagg aaaaagtata
     3961 aaagctataa acttgtatac taaagaagag tgggtttttg acactcatat acagtgtagt
     4021 aaaaaactca aagttccatt aggatacata agagaaaatt taaaatatgg atatatggat
     4081 tactttggag atgcaataaa ttatttaagt gaagtattaa atatagatga atactgcaaa
     4141 ggtgaatgga gctatctcga taatagcaaa tctccatctg agatatttaa tattctaaac
     4201 aataaaatat ttagtataag gctttctaat gaaaaaagaa atgaaatttt gacaaatgat
     4261 aaaattgaag cattaaaaat gaattataga tttgaatgta ttgatgaaga atatgatgaa
     4321 tattttaaaa aatataagtc tataatcaaa aggggtggaa agaaaaaagt tgaattagta
     4381 aataaaaaag gtgacatttt agaaatattt aagtctttag aagaatgtgc catttatttg
     4441 caaaaggaaa agaatgaagt tatacaaatg ttaaaatatg gagatacaaa agtaggaaga
     4501 aactttataa ggtatagttt gagaagtatt taatttcatt ttaaattcat aagagtttca
     4561 tttctgattc acacatttaa tattatttta atgtaaaata ataaaggtga agtattgcac
     4621 aattaaaatt aaggagggta ttaatgtctg aagaaaaaaa tccaaaagaa aatacattag
     4681 atgaacctaa aaattcagat aatgtaaatt ctaaaaaaga tgatgtgtca gatgaaaaaa
     4741 atactgataa caaagttaaa aaaagggaaa taaagctaaa caacaaaaca cttaaagcta
     4801 ttgtagcaat tgttgcagta ggagctatat gtttaggtgg aggctatgtt tttggtaaag
     4861 aagctggaag aaaattgcct gctacttcaa aacactataa tagcagtgag gtagtagcta
     4921 ctattgggga taaaaaaata acaggtgaaa tgttaagaaa gagaatggag ccattatttt
     4981 atttaaatgc aaaaactact atgactgatg acgaaatacg ttcatatgaa tcaaatatga
     5041 ttgaaactat gattacatct gatatattat cctctgaagc agcaaaagag aaaataagta
     5101 taactaatga ccaactagat gctcagtata ataatttaat gagtagtata gaatcagcaa
     5161 tgggaatgac taaagaagag tttttaaaac agtttgattt gactgaaaaa tatataaaag
     5221 atgatatgaa aaaagagcta gtagcatcaa aatatttaaa tgaaaaatca aaagttactg
     5281 ataaagaagc agagaactat tacaataaaa acaagagcaa ttatttacaa gttagagctt
     5341 cacatatttt aataaaaact gttgatgata aaggtaagca agttagtagt agcaaaaaag
     5401 ctgaacttaa gaaagaagct gaagaaatac ttaaaaaagc ccaagctggg gaagattttg
     5461 ctactctagc taaaaaatat tctgaagata gtagtgcaga atcaggtgga gatttaggtt
     5521 tctttggtaa aggtcagatg gtggaatctt ttgaaaaagc agcctttgca ttgaagaagg
     5581 gagaggtatc taacaaactt gtagaaagtg attatggata tcatataatt aagaaaactg
     5641 atgaaaaata tcaaccattt gaagaggtaa aatcagattt agtaagcagt ttaacatcag
     5701 aaaaacaaaa cctacttata caaaatttaa aagaaaaata taatgttaaa attacagata
     5761 aatatcaaat gaaagctaaa gaatagataa agtcaattta ttaaaaaaaa gaaaaattaa
     5821 ctgtatggat aaaagctatt tttactctca atatagagag tctaaagtag cttttattat
     5881 tttgtaataa aatctattta tatatatgaa gtgataaata aataacatgc aaaaaaatat
     5941 taaaaagaaa tatttttaat ttgaattttt ttaatgaaaa
//
LOCUS       DAGQWR010000194         5969 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.197, whole genome shotgun sequence.
ACCESSION   DAGQWR010000194 DAGQWR010000000
VERSION     DAGQWR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.197"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..409
                     /locus_tag="MIW95_003564"
     CDS             <1..409
                     /locus_tag="MIW95_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBY3152859.1"
                     /translation="YSKNHNEAYKTFLFLENESLKSNITYCFFDSFLEMIDNENSYIR
                     TRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPNITKYKENLINR
                     IKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
     gene            1040..2347
                     /locus_tag="MIW95_003565"
     CDS             1040..2347
                     /locus_tag="MIW95_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152860.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLVLDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQSKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    2433..2695
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2821..3624
                     /locus_tag="MIW95_003566"
     CDS             2821..3624
                     /locus_tag="MIW95_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY3152861.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINKTLKEMKKDGTFDKIYDKWFK"
     gene            3662..4273
                     /locus_tag="MIW95_003567"
     CDS             3662..4273
                     /locus_tag="MIW95_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBY3152862.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            4305..4976
                     /locus_tag="MIW95_003568"
     CDS             4305..4976
                     /locus_tag="MIW95_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBY3152863.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            5275..5637
                     /locus_tag="MIW95_003569"
     CDS             5275..5637
                     /locus_tag="MIW95_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBY3152864.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         2436 a          526 c         1002 g         2005 t
ORIGIN      
        1 atattcaaaa aatcataatg aagcatataa aactttttta ttcttagaaa atgaaagttt
       61 aaaatcaaat attacatatt gtttttttga ctcattccta gaaatgattg ataacgaaaa
      121 ctcatatatt cgaacaaggg gattgttatt aatctctgca aatgctcagt gggatattga
      181 taataaaatt gaaataaata tagatagtat actaagtcat atcgtagata aaaaaccttc
      241 tgtatcaaga atgtttatta aatccatacc taatattaca aagtataaag aaaatcttat
      301 taatcgcata aaaatggaat taagtaatgc agatataagt atttataata ataatatgaa
      361 accattagta gaaaaggata taaacgatac attatctaat attagttaaa tattaatata
      421 gaaattgaat attatctagt tacaattatt tataactaag ataataaaac aacaatggtg
      481 ctaatttata tgtataaatt aattatgaaa aatttatata agtaaaatat attaacataa
      541 aggtaaaatt ttgtatacta aaagggaata tttacagttt aatggcagtt ttaaaataat
      601 aaattaaagc tgtcattttt atttaaaaat ttttagaaaa agtacaatag tgttaatgtg
      661 tgaattagat aaggtgtata taataattta gatgatatga tataaaatat gttgtaaatt
      721 aaatgttttc tttgacaata tatacacaag gatgataata tctatatgaa taaaagatag
      781 tgtgtaattt gataatatgt aattataaca ttatgatata attacaataa agtatgtaat
      841 aatctgtaga aatttcaagc tagttatcta agttttcgga taactaaatc taaacggaat
      901 acataatgta ttaaataaat gatgatacta aaatgatact atttttatag aaatttattg
      961 aggtatataa ttgatgaaaa taatatatta aattatataa tatattgagt tttacataaa
     1021 agcgaaggtg ggaataaaaa tgaaagaaat tgctgatgct ataacatcag agtatttaag
     1081 ctatggtatc aatgaagata ttagtatcta tgaaggtaga ttttgcatat atttagataa
     1141 aaaatataga tgtaatggga aaatttatta taaaatgaca cctccaatat ctataagttt
     1201 taaagcagac ataggttgtg ttgaagagat agacaatgaa gatgacaatt tagttttgga
     1261 ttatgataat gctattcttg aagttcatgg atacaaaatt atttctataa caataaatac
     1321 tttaagtgaa tttagtgtag aaggttatat caatgatgac tgcataaaat ctaagaactc
     1381 ctatgttgaa tatgtagatt ttaatataat aaacttagat aaaatacctg gaaaactaat
     1441 taaatacaat gataaggtat acgcaggtag aattgaattt gatataaatg attatgttgt
     1501 aactatagac aagcgatatg attatagaaa agaattaaaa tcagaattaa agtctaaatc
     1561 tggagcaata ataactcata taggtaggat tagaagaaaa gatggtagaa tttttagaac
     1621 aaataataca ataaatcttt tagatagaat atcaacagct ttgagtttta tgtgtggaag
     1681 atatgtaggg ttttgtttgg ccaaagggta tagaagtggt aatgaagttt atagaatatg
     1741 gaatgaaaat caaatatctc cttttagata tgtaccaaca tggtcagata cactttcaaa
     1801 ttatcataat atggaaaaat atataagcct tatgtgtaaa aagctagaag atttttatta
     1861 tggttctgct ataaagagtg ttgttgattg gtacatagaa tctcttggaa gtgctaccat
     1921 ggaaaacaat ataatatctg tacaaattgc acttgaaact ttgtcatatg taattctagt
     1981 ggaacaaagt aagatactta cagatgaagc atttgactgt aatttggcat ctaaaaatat
     2041 aagactctta cttgatacat gtaagatacc atatggtaaa catgaattaa atatatttga
     2101 taatataata aaaaataaat ttgatgatgg agtggattta gttatttatt taagaaatag
     2161 catagttcat ccaagtagaa agactcatag agctgtgtta gaggttgaag atatttggaa
     2221 tataatttca ataggaacga gatatattga acttgtttta ctctttattt taggttatag
     2281 aggagaatat tcaaacagat tagtagaaag gtgttatggt gaagtggaag ttgtaccttg
     2341 gaactagatt tttatattta ttttaaaata tatattgaca tatattgcct tatgctataa
     2401 aatcaatatc ataataataa ttcagaataa cattaaatgc taagaaaaga aatagtaaag
     2461 gtagttaatc ttaaacagag agtttccgat tggtgagagg agcaatagat tttactttga
     2521 acacatcttt gagcaggact ctgaaagagt agaaatactt tattaggaga tttccggaat
     2581 ccttgcaccc gttatagtgc tagagtataa taattttatt gtacttaaag aggttagtgt
     2641 cgtgaggcat taatgaacag aggtggaacc acgagagtta actcccgtcc tctagcagaa
     2701 gctagaggcg ggagtttttt aatataattt tttaagcgcg cagagaatta tattgcaata
     2761 gatacacttt ttaaaaaata atcaaaatat tttaaaaatt aaaaggcgag ggagaatatt
     2821 atgaaaaata tattaaaaaa agtaggaata tttacaatta tgttaggttt attaggtgga
     2881 gttgtaggtt gttcaaaacc agataatgaa aaagataagg atgcttcaaa ggaatctaaa
     2941 aaagaagtag ttgtagggtt tgataatacg tttgtaccaa tgggattttt agatgaaaag
     3001 ggagatacag taggatttga cgtagactta gctaaagaaa catttaaaag acttggaatg
     3061 gaagttaagt ttcagcctat agattggtca atgaaggaaa ctgaattaaa tgattcaaaa
     3121 actgtagatg tattatggaa tggatattct ataacagacg aaagaaagaa aatagtatca
     3181 tatacagaac catatttaca aaataaacag ataatagtta ctctaagtga ttcaaaaata
     3241 aattcaaaag ctgatttaaa agataaagaa gttggaacac aacaaggttc aacagcactt
     3301 gatgcagtgg aaaaggataa agattttatg aatagcttaa aaggtggagc accagttctt
     3361 tatgatactt atgataaagc acttagagat ttagagatag gtagaactag tgcagttgtt
     3421 ggtgatgaag ttttaattag atattatatg ggacaaaagg gtgaagacaa atacaaggta
     3481 ttaaaagacg attttggatt agaagattat gttgtagcaa cttctaaaga aaatccagaa
     3541 ctttgtgaga aaataaataa aacattaaaa gaaatgaaaa aagatggaac ttttgacaaa
     3601 atttatgata agtggtttaa atagaagata agttagctgt taagaagggg gcactaaagg
     3661 catgttacaa ggtttagaga tagttatagc aatgttttgt ataacattga tagtatcaat
     3721 tccattgggg attggagttg catttttaag attatcaaag aataaattag taagtggtat
     3781 tacacaatgt tatatattga taatgagagg tacaccttta cttttgcaaa tgatagtcat
     3841 attttacgga ttacctcttt taggaattgt atttgacaga tttacagctg gagtagtagc
     3901 tttcttctta aattatgcag catattttgc agaaatattt agaggtggaa tacagtcaat
     3961 cgatagagga caatatgaag cttcaaaagt actaggattt gataaattta ctatgtataa
     4021 gagagtaatc tttccacaag tttttaaaag aatactagca cctatttcaa atgaagtaat
     4081 cacattagta aaagatacat ctttagtata tatattagga ttaaatgata ttttaagaat
     4141 atctcaaata gcaatgaaca gggaagcaag tctgttacca ttatttgagg caggagcgat
     4201 atatcttata tttgtagcta tacttactaa gggatttgaa ttacttgaaa aaaaatattc
     4261 ttattataga taaagttaca atattatagg aggaggtttt ttatatgttg aaaatcaaaa
     4321 acttaaataa atcatttaaa aaaaatagag tacttaaaga tatttctttt gagttagaag
     4381 aaggtcagat tggagtttta cttggtaagt ctggtgcagg aaagaccaca atattaagat
     4441 gtataaatgg tcttgaagaa tttgatagtg gagagattat aatagataat gaggtaataa
     4501 aaaataagag agatatggca aaaatacgag gaaaaatagg tatggttttt caaaatttca
     4561 acttatttcc tcatatgaca gtccttgaaa atataattga atccccagtt aatgttttta
     4621 aagttcctag aaaagaggct gaagagaggg ctagagaact tttaagatta gttgatttag
     4681 aagataaatt aaattcttat ccatttgaat tgtcaggagg acagcaacag agagttgcta
     4741 tagctaggtc atgtgcattg atgccaaaag tcctttgttt tgatgaacct acttctgcac
     4801 ttgatattga tactattcaa agagtagtca atataatgaa tagacttaaa gacaaaggta
     4861 tgacaatatt aataattaca catgatgttg tcttctcaaa taatgttgca gataaaatta
     4921 taagtataaa agatggtata gtagaaaatg ttcaaataaa agaaaaaatt gtttagagtt
     4981 ttataaatat aaactagggt agaattttat aatataaata aagggcaagt aaaatcaaat
     5041 atactatgcc cttttgttat ctcaaaattt aatcttatat tttatgttta agttacatta
     5101 taatttataa taaaaataaa aggactattt aaatatataa tgtgaataaa tgtttaataa
     5161 tttgaaatat gataattttt tatgttgaaa gcttataatg attatgatat aaattggtta
     5221 tagtatacaa tttatgggta taacaataaa aaattagaaa ggaattttta taaaatgatt
     5281 aaattatatg gatatacaaa atgctctact gttaaaaaag ctaaaaattg gcttaaggaa
     5341 aataatttag aatttgaaga tatagatatg gttcaaaacc ctcctagtaa agaggagtta
     5401 aaatcaattt ataagactag tggatatgat ataaagaaat tttttaatac aagtggtatg
     5461 aaatatagag aattgggtct taaagatatt gtaaaaactg aatctgatga taaactttta
     5521 gaaatattgg taagtgatgg tatgcttatt aaaagaccat tacttttgga tggtaaaaat
     5581 gtattattgg gctttaaaga agatgtatgg aaaagtactt tgctaaaaga agattaggaa
     5641 gagataggtg tgttgattaa aattattaat taatacacct ataaatttat gtattaaatc
     5701 taatctttat aaatattaat tagcaaattc aaatcaaaaa aattcatatc aacaaatata
     5761 ttacttctta ttacaaatta tatcatatgt tttatattaa aatattataa tattacaacg
     5821 aattgttaca aaattactta ttattaacca tgaacatttg ttttgtgcta tcatatttat
     5881 ataagggaat tgtcataaaa aatatcttgt tcatacagtt ttatatagaa aaaagaaatg
     5941 aatcttttct ttatataagt atattttgt
//
LOCUS       DAGQWR010000195         5922 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.198, whole genome shotgun sequence.
ACCESSION   DAGQWR010000195 DAGQWR010000000
VERSION     DAGQWR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.198"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            144..1508
                     /locus_tag="MIW95_003570"
     CDS             144..1508
                     /locus_tag="MIW95_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBY3152865.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSNFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVENTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1647..2813
                     /locus_tag="MIW95_003571"
     CDS             1647..2813
                     /locus_tag="MIW95_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBY3152866.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2930..3712
                     /locus_tag="MIW95_003572"
     CDS             2930..3712
                     /locus_tag="MIW95_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBY3152867.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3831..4484)
                     /locus_tag="MIW95_003573"
     CDS             complement(3831..4484)
                     /locus_tag="MIW95_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY3152868.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4508..5872)
                     /locus_tag="MIW95_003574"
     CDS             complement(4508..5872)
                     /locus_tag="MIW95_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBY3152869.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2226 a          750 c          904 g         2042 t
ORIGIN      
        1 tacttaataa ggggcttttt ttattggaag atttatgtta tattgagatg aatttattat
       61 gatataaaaa ataatgttag gataaattta aatctttttc taaaaatgta ttaatataat
      121 tcttaaagaa aggattttta acaatggata taaaagagtt agttaaaatg caaagaaaat
      181 attataatac aggtaaaaca agagatatat cgtttcgtat agaacagttg aagaaactta
      241 agttagttgt atcacaaaat gaagaaaaaa tacttttagc actaaagaaa gatttaaata
      301 aatcgaattt tgaaggtttt atgacagagg taggcatgtt ttatagtgaa ttaaactttg
      361 ctataaagaa tataagaaaa tggagcaaaa taaaaagagt taaatcaagt atggttaatt
      421 ttccatctat atccaaagtt gttccacaac cttatggtgt tactctcata atgtcacctt
      481 ggaattatcc ctttcaatta gcacttatac cattagtttg gtcattagca gcagggaatt
      541 gtgttatatt aaaaccttct gaatattctg tgtcaacatc tagtgtagtt aaagacatag
      601 ttgaaaatac tttttcaaaa gaatatgttg cagttgttca aggaagtcaa gaagaaagtg
      661 aaaagttact attggaaaga tttgattata tattttttac aggaagtact aatgttggta
      721 aaataatcat gaaatcagca tcagagcatt taactcctat tactttagaa cttggaggaa
      781 aaagtccttg tatcatttta aaagatgcta atatagacct tactgccaaa agacttactt
      841 ggggaaaact aataaatgca ggacaaacct gcgtggctcc agattatgtt ttagtgcatg
      901 aagatagaaa aaatgagtta atagagaaaa taaaatatta tactaataag tattttggag
      961 ataatccatg taataatgag caatttccta aaatcataaa tcagagacat tttaatcgga
     1021 tattatctct tattgataaa gataagatag tctatggtgg aaattataat aaggagacat
     1081 taaagataga gccaactata gtagacaatg ttaattggga tgataatata atgaaggaag
     1141 aaatatttgg gcctatattt cccattttaa catataaaga tttagatgaa gtaatacaaa
     1201 aaattataca aatgccaaat ccattggcac tttatatttt tacaaaaaat aagtacctag
     1261 aaaataaact tttagaaatg attccagcag gcggatgttg tatcaatgat acagttactc
     1321 atatagctac gaattactta ccttttggtg gtattggtga aagtggtatg ggctcatatc
     1381 atggaaaagc tggattcgat acatttactc actataagag tgtgttaaaa aaattaaatt
     1441 tggatgtccc tattagatat gctccatacg ataataaatt aattaaggtt cttaaaaaga
     1501 taatgtaggt tatattgtag aataataaat ataaataaaa ttgagtattt gtattctgaa
     1561 tatattttat ttttaatttg gtatatttgt tataaaagtg gtgattttgt gataaaattt
     1621 aattatatag aaataaggag gaaaaaatga aaattaattg gaatacaaaa tacacaacaa
     1681 tagcaattta tacatttata atagctgcta gtagtattat attttatcta gtatcatcac
     1741 aaattgatgt attttctaat aatttagatg ctatatttac aacattacaa ccttttataa
     1801 taggatttgc tattgcatat cttcttaatt ttattttaaa gttttatgaa gacagaattt
     1861 ttataaaaag tgagaaatta aaaaaactaa aacaaagttc aaaaagaggc ttgggtcttt
     1921 tattaacata tgcaacagct acattgatac tttacttatt tatgcatttc gtattgccac
     1981 aagtaattga gagtattgtt ggactagcaa atgatatacc tatgtatgta aacaatgcaa
     2041 ctaagcttat tgataaatta atgactgatt taaatcttga tgagcaatac tttaatttgg
     2101 cagttgataa gtggaatgag tttgttacat atattataaa gtttgtaaca gatttaatcc
     2161 caatattagg aagtatgtta aagaatgtag cctctagtat atggaatatt gtactaggat
     2221 taattgtttc agtatactta ttaattgata aagaaaaatt ctatggatta agcaaaaaaa
     2281 taacttatgc agtatttaca gaaaaacaag ctgccagaat tttagaatta actcatagaa
     2341 gtaactatac atttggaaga tttttaggtg gcaaaatatt agattcattc attataggta
     2401 ttctaacatt tgtaatactt acattagtta aaatgccata tacattatta atatcagtta
     2461 taattggtat tacaaatatt atacctttct ttggaccttt atttggagct ataccatcta
     2521 ctctaatagt gatgtttgta tctcctataa aagctttctg gttattgttg attatattaa
     2581 taatacaaca gatagatgga aatattatag gacctaaaat acttggagat tcaattggta
     2641 tatctgcatt ttggattttg ttctccttat tggttgcagg aaaattatta ggttttattg
     2701 gaatggttat aggagttcca atgtttgcag taatttattc tataattaag gatattgttg
     2761 aaagtaaatt ggacaaaaag ggattaccga cagatacttc cgattacatg tagctaaaaa
     2821 atattatgta taattaaaaa tatataaatt aagcaatatt taaagcactg agaaagttaa
     2881 actcttggtg ctttttagat ataaaattat tatagaagag gtgttttttg tggcatttat
     2941 agaatgtaaa tttaaatcta aatatactgg aggagaaact gatataattg taattttgcc
     3001 tgtagcaaat ggaaaagaac tttttagtgg taaagagata tatgattttg aaggtaaatt
     3061 taaaacatta tacttatttc atggtttaga agatgaccaa agctcatgga ttagatatac
     3121 taatgtagag cattatgcta aaaataaaaa tatagcccta gtaataccaa gagtagaaac
     3181 tagtttttat acagatatgg cgtatggaca ccaatatttt acttttgtaa gcgaggaatt
     3241 acctaaattt gttagagcta tatttccttt atcagataaa agggaagata actttgtagc
     3301 tggtatgtct atggggggat atggagcttt aaaattagca ctttcaaaac caaaagagtt
     3361 ttcggcagtt gcaagttttt ctggttcgcc tgatatgatt catgagttag aaaatcaaca
     3421 aatagaaacc aatgcagaca ttttatttca cgattttggt agaccagaag acgctagaaa
     3481 tagtgagaat gatttacttt acatacttaa gaaccttaaa gaaagaaatg aagatattcc
     3541 taaaatttat caattttgtg gtgatagaga ttttttatat aaaaataatc agatgtttaa
     3601 aaaatatgca gagtctctgg gtgtagatgt tgaatatgaa gagtgtgtag gggggcatga
     3661 atggcgtctt tggaatttat ggataaagaa atttatagat ataattgggt agtacactta
     3721 tggtattaac aggtatacta taaaatatct ttaaatatga taaagactga aatcctaaaa
     3781 tcaaattatg attttaaaat ttcagtctat aagaatttaa taaaatttta ttaagttctt
     3841 acacgagtta ttattggagt aaaattgtca actatatcta tatagtattt actcaattcc
     3901 tctaaaatct cttcgcataa atccgtacta tgaccaagat taataagaaa ctgttttgta
     3961 aaatgcttta ttacagcttg agaagatata tcgtacttgt gtgaagtata aactctaatt
     4021 atttcaggtt tcatagcaag tataccaata caagaaagta ttaattcatc atgttctata
     4081 gattctttat cattaacata tcttttcatg accatttcgg ttacacttct ttgatagctt
     4141 aaaatagctt cttcataagc tatatccaaa taaaaatttc tatatgcttc gttttttaga
     4201 aggaaacaaa ggtcagcatt catagcaaga aagaacatca tcatagtacc ttcttcatgg
     4261 tagttataga ttaaagactg catttcattt cttatatctt catatattat agctcctatt
     4321 tcgcttttac ttgagaagta atagcttatt agacctaaat ttatattagc tctttctgca
     4381 atttgcctag ttgttgtctt atgataacct tgctcaaaaa ataattctct agcagcactt
     4441 ataatactag cttttgtttc tttgcccttt tctgattttg acatatttac tgtgtatacc
     4501 tctcttttca atcttttttt gaaaatttgc taactataaa tattgtaata gaaccaccta
     4561 ttataggaac caaaattctg tcaaatacag taagaatacc aatcatattt aataatgcca
     4621 atataacact tactataaca gcatagatag ctcctttttt tgtagcgcct ttccagcaaa
     4681 gaccaattac aaatggtgca aatattacac ctgtacataa tggagccggc caaaacaata
     4741 attctaataa actatcaaat ttaagtgcta tgattgcacc tattatagct gtcaaagctg
     4801 atgcaatttg agaatacttt agagtatttt ttaaatcatt aaattctata ttaggatgta
     4861 ataatttgtt ataaatatct cttgatataa tagcactcat tgaggtcatt aatgaatcgg
     4921 cagtagacat tatggcagcc aatatagcag ctaagaataa tacagatatt aaaggtggca
     4981 aagtagtcga tatcatttga ggtaaaacct gaacagatga tatgtttgga aataaactac
     5041 caccaatagc tccaattatt cctataggga aggcaagaaa taatagtata aatcctgcaa
     5101 ttacactact tttaactgca tttttagcat tatcagcaca ttgatatctt aaaaaacttt
     5161 cataagaaaa tattgagcct aaaactaatg gtaaaactgt atacataaaa tcactaatcg
     5221 atttatttcc tggaaagaat ggttctttta ccattgattg taacataagt aaagggttgt
     5281 cgccaactaa ttttaagctt atataagcta ctattggcaa aaacactata caaatagcaa
     5341 gttgaataaa tgctgatact gcaactcccc acattcctga tgtataagta tatataatta
     5401 taactataac tcctataatt acagctatat cataaggaat accaaatgca gtagatacag
     5461 aaccaaaaga aattaattga ctacccataa tagctagtat agatattggt gttacaagac
     5521 tttgataagc tcttaaaaat acactatgac catatctagt ttcaatataa tcagcaggtg
     5581 ttatatcaca agataagctt cttaattttc ctgcgaaaaa ggcatttata aagcaagcta
     5641 aagctgcacc tatagtagaa tacattccaa cccaaagtcc tctttcagct ccccattgaa
     5701 ctccaccaac taagcaccct cctccaaaag atgtagcagc aatggttgca gctagtatag
     5761 gcaaaggaag atttctacct gcgattagga aatcggatgc tgttttaact ttttttgaaa
     5821 aataaaaacc aatccatatg tatattgcac acgtaacaat taaaagtaac atagtctttt
     5881 ctcctttata aaatatttta ataaaatata taaaatatat tt
//
LOCUS       DAGQWR010000196         5845 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.199, whole genome shotgun sequence.
ACCESSION   DAGQWR010000196 DAGQWR010000000
VERSION     DAGQWR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.199"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..342)
                     /locus_tag="MIW95_003575"
     CDS             complement(<1..342)
                     /locus_tag="MIW95_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBY3152870.1"
                     /translation="MREGKRRRGCLFWFIIIIIFSGVVGAIAGNSTNNGSTEKQKEDL
                     TKYIGEEGNIGDLKLTVNSISKASEIPVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPD"
     gene            complement(402..635)
                     /locus_tag="MIW95_003576"
     CDS             complement(402..635)
                     /locus_tag="MIW95_003576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152871.1"
                     /translation="MNKDIELTEKDLYCIARHIQVYVFKKDDEVIKKEDNPCCRCKHK
                     FKYYENSVCIYHCSVFQKLSKITGLKMGFGVKL"
     gene            complement(784..945)
                     /locus_tag="MIW95_003577"
     CDS             complement(784..945)
                     /locus_tag="MIW95_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152872.1"
                     /translation="MDNFLLSILASLIASLIGYIVCRCIKNVKSHSTRGKSKSGWELG
                     FKIKFRKFK"
     gene            complement(1266..1706)
                     /locus_tag="MIW95_003578"
     CDS             complement(1266..1706)
                     /locus_tag="MIW95_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBY3152873.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKAIEWELKAIDSD
                     RDRQLRKDSTIRVPVLNKKGKATGQYTSETDFNTYTLKLCVETVVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            complement(1711..1968)
                     /locus_tag="MIW95_003579"
     CDS             complement(1711..1968)
                     /locus_tag="MIW95_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152874.1"
                     /translation="MIKIKDKYEIKDSISFDYSNKRPLEERVSEMYKKAGKYLIDISD
                     KLATDTIDGSSLKPIIIKFEINEAGVATIEKQTKYLVMEVE"
     gene            complement(2021..2491)
                     /locus_tag="MIW95_003580"
     CDS             complement(2021..2491)
                     /locus_tag="MIW95_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBY3152875.1"
                     /translation="MAQQIKARDTISASKAECFVTIKGKRYNFMQAINLEAKMEKNKS
                     EIPILGSTTKGNKSTGSKYSGNATFYYNTSIFRELLYEYKETGEDIYFDIQITNEDPT
                     SSVGRQTIILEDCNMDSGIIAKFDADGEYLDEDMDFTFENWKLVEKFNIANGME"
     gene            complement(2513..3823)
                     /locus_tag="MIW95_003581"
     CDS             complement(2513..3823)
                     /locus_tag="MIW95_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBY3152876.1"
                     /translation="MALGGGTFVTQNKVLPGSYINFVSATRATSSLSDRGIVAMPLEL
                     DWGIDEEVFQVTSDDFEKYSTKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CSIATARCSGIRGNDLKVIVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITELQDNDYV
                     TWKKEATLEATAGLTFTNGTNGEAVTGTEYQAFLDKIESYSFNALGCLATTAEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKNDADYEGVVSVENKIKDTGLLESSLIYWTTGAIAGC
                     DINKSNTNKRYDGEFDVDVNYTQIHLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     EKNDDFSSNQSVRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMSKLYMTVSVS"
     gene            complement(3824..4000)
                     /locus_tag="MIW95_003582"
     CDS             complement(3824..4000)
                     /locus_tag="MIW95_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152877.1"
                     /translation="MSKTLSKGTDYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            complement(3993..4430)
                     /locus_tag="MIW95_003583"
     CDS             complement(3993..4430)
                     /locus_tag="MIW95_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152878.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEAKNFEINEIAEMLLEELEYIEINGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNNADKMNTVELFGGLKRGDNFE"
     gene            complement(4423..4848)
                     /locus_tag="MIW95_003584"
     CDS             complement(4423..4848)
                     /locus_tag="MIW95_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBY3152879.1"
                     /translation="MTRWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            complement(4848..5195)
                     /locus_tag="MIW95_003585"
     CDS             complement(4848..5195)
                     /locus_tag="MIW95_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152880.1"
                     /translation="MVSKTRKAIEMLYRYKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(5189..5590)
                     /locus_tag="MIW95_003586"
     CDS             complement(5189..5590)
                     /locus_tag="MIW95_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152881.1"
                     /translation="MGNNIIDDIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEADMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTAYGKNKILTFRCLRW"
     gene            complement(5600..5833)
                     /locus_tag="MIW95_003587"
     CDS             complement(5600..5833)
                     /locus_tag="MIW95_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152882.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKVVRYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
BASE COUNT         1751 a         1174 c          582 g         2338 t
ORIGIN      
        1 atctggtcct attaatgtga atgaatttag cataaaagat tggctttcct tagtttgatt
       61 ttttattgtt aaatttataa taccatattt gccactatct ggagtgtacg ccaaataacc
      121 agaagctact ggtatttcac tagcttttga aatactatta acagttaatt ttaaatctcc
      181 tatattacct tcttcaccaa tatatttagt taaatcttct ttttgttttt cagtgcttcc
      241 attattagta ctgttccctg ctattgctcc aacaacccca gaaaatataa taataatgat
      301 aaaccaaaat agacatcctc ttcttctctt tccttctctc ataatatccc cctaaattat
      361 attttataag aatattatac tatatattca aaatttttac attatagttt gactccaaaa
      421 cccattttaa gacctgtaat tttagataac ttctgaaata ctgaacaatg atagatacat
      481 acactattct cataatactt aaatttatgc ttgcatctgc aacatggatt atcttccttt
      541 tttatgacct catcatcttt tttaaacaca tacacttgaa tatgtctagc aatacaatat
      601 aaatcttttt ctgtaagttc tatatcttta ttcattcaat caccttcttt ttcacataaa
      661 aaaagtactt actattaaaa caagtacttt gcatattttt tctaatttgt ggtataataa
      721 aggcaagaag aactacaatc tatttagcgg tagagtgaag ttcataattt taaaaaatat
      781 aaattattta aatttgcgga actttatttt aaaaccaagt tcccagccac ttttactctt
      841 gccacgagta gagtggcttt ttacgttttt gatacatcta caaacgatat atccaattaa
      901 actagctatc aagctagcta atatactaag taaaaaattg tccatacttc ccacctcctt
      961 tcattaggaa gtaggtttta tcccagtatg aactccactc tataaattgt agattacatc
     1021 ttcttgctac aattattata acatataatt cttacatatt ttacctatat tttatcttct
     1081 tctacttctt ctagcttctt tagcaatctt tttttcttct tttatttctt cttctacttt
     1141 aatatctata gaagcagcaa caaatgccct ctcgaagtct ggtaaatctg tatattcatg
     1201 tggtttccat ttgaatttat gaaggcaata atgagctaca ctagcatcat aatcgcctcc
     1261 ttcaattagt tttttgcttc ttctacttta tcttcaaaag tcctatcaaa accattcact
     1321 tctcctacct cacttgaaag gtctgtgtat tcaccaggag ttaacattgt tgttaatagt
     1381 tcctctgctc ccattacacc atagctattt tgaagttctg catcatgtaa atctggaaat
     1441 actacagttt ctacacacag tttcaaagta taagtattaa aatctgtttc actagtgtat
     1501 tgccctgttg ctttcccttt tttatttagt acaggtactc ttatagttga atcttttctt
     1561 aattgtctat ctctatctga atctattgct ttaagttccc attcgattgc ttttccatct
     1621 tctcctataa acctttcact tgccacatac tttctattct ctacttttat tgcattttga
     1681 cttaaaaaag cgtttaaatc tcccatattc ttattctacc tccatcacca aatattttgt
     1741 ttgtttttct attgttgcaa caccagcttc atttatttca aattttatga ttattggctt
     1801 taatgacgaa ccatcaattg tatctgttgc taacttatct gaaatatcta taagatattt
     1861 tcctgccttt ttatacatct cgctgacacg ttcttccaaa ggtcttttat tgctataatc
     1921 aaaagaaatt gaatctttta tctcatattt atccttaatt ttaatcatct cctattctta
     1981 taaataaaaa atgcacatac ataaatcata aatgtgtatt ttactccata ccatttgcta
     2041 tattaaattt ctcaactaat ttccaattct caaaagtaaa atccatatct tcatctaaat
     2101 actccccatc agcatcaaat ttagcaatta tgcctgagtc catattgcaa tcttccagta
     2161 ttatagtttg acgacccact gaacttgttg ggtcttcatt ggtaatttgt atatcgaagt
     2221 aaatatcctc accagtttct ttatactcat acaacaattc tctaaatata gaggtattat
     2281 aataaaatgt tgcatttcct gaatatttac ttcctgttga tttatttcct tttgtagtac
     2341 tacctaatat aggtatctca ctcttattct tttccatttt agcttctaag ttaatagctt
     2401 gcataaaatt atatctttta ccttttatag ttacaaaaca ctctgcctta gatgcactta
     2461 ttgtatctct tgcttttatt tgttgtgcca ttattattct cctttcttat tgttaactaa
     2521 ctgaaacagt catataaagc ttactcatag cactaataac ttttacagca tcacttacta
     2581 caacagtctt cttgtcgctt ccaggttcta cagaaacatc atcagctttg aaatcttcta
     2641 ttgctctcat attttgcagt tgttcatgat gcttaactac atcattccaa aacgagatac
     2701 gaccagattt atcatttggt acttcaccca agtactttgt attaaataaa gtcgctatat
     2761 cattagcaat ctggtcaagt actctaacac tttgattact tgaaaagtcg tcattctttt
     2821 catctgtaaa actaacaaaa gtatttatat cctctaatac atgaacttca tctccaacct
     2881 tatgaaatat aaacttacca gtttttaaag cttcttctaa atgtatttga gtgtaattaa
     2941 catctacatc aaattcacca tcatatcgct tgttagtatt agatttattt atatcgcatc
     3001 ctgctatagc tccagtagtc cagtaaatta agctagattc taataatcca gtatctttaa
     3061 tcttattttc aacagatact acaccttcat aatctgcatc attcttttta tataatacag
     3121 tttgaaactt agccccaact ttgtctctca ttctcttagt aaactctaca aacaaacttt
     3181 taatctctgc tgttgtagcc aaacatccta gtgcattaaa tgagtaactt tctatcttat
     3241 caagaaaagc ttgatactct gttcctgtca cagcttcacc attagttcca ttagtaaaag
     3301 ttaatcctgc tgtagcctct aatgttgctt ccttcttcca agtgacataa tcattatctt
     3361 gcagttctgt aataacttta gctatttgag tatcaacttt cttattatct aaaagtgtta
     3421 caacatcaaa tttagcatta tcatctatat ttgttgttac tataactttt aagtcattac
     3481 ctcttatacc actgcatcta gctgtagcaa tactacaact agctttaaca cctttattta
     3541 atttatagaa atatcctagc cttatatttt taaataaatc tctcaaacct ttcaacttct
     3601 catgtgtata atcatatcca aaatacttag ttgaatactt ctcaaaatca tcactagtca
     3661 cttgaaatac ttcttcatca attccccaat caagttctaa aggcattgca acaatacctc
     3721 tatcgctaag tgaactggtt gcccttgtgg cacttacaaa gtttatatag ctacctggta
     3781 atactttatt ctgtgttaca aatgttcctc cacctaaagc catctaactc actcctttca
     3841 tgaatttatt tattctatcc tctacttctg agaaggaata taactcattt tcttttaaaa
     3901 ttgcatttaa taagtctttt ctatttacat acttcttaga attaactatc tgctccttag
     3961 taaacttgta atcggttcct ttgcttaatg tcttactcaa aattatcacc tctcttcaaa
     4021 ccaccgaata actctactgt attcatttta tctgcattat tactctttat agtgaagtag
     4081 ttataatcaa caaagaagtg aagaacattg tctataattt caaaattcat atttgtgcct
     4141 ctaactaaat ctccattgat ttctatatac tctaattctt ccagtaacat ctcagctatc
     4201 tcatttattt caaaattctt agcttctgaa cgagggaaat aatgtacatc aaaagaattt
     4261 ttctttaatt ctctcccgct tggatatggt gtcttgcttg gatttaaagg aacaataaaa
     4321 aaacaaggtt cattaatacc ttgctctaca tcctcactat aaattgtata tttttctcca
     4381 aatgatttat ctaactttac tgatattcca tctataatat tattaagcat caaatactcc
     4441 tttaagtaat attaataact ttttctctat aatcttatca acttggcttt gtagttccat
     4501 ctctgaaatt gttaagaaat gttgtccttt aacccaacct tttccgtctt tagttctatg
     4561 cccgaaattt acataacttg catattcagt cggattaaca acctctataa tataattatt
     4621 tccttgttta tacacaggaa gcgacctagc ataagccact ccattccatc cttgtcttaa
     4681 gaatcctgta tcaactggtg tccttctaat tacttttcct aagagtcttg ctgctaattc
     4741 tcttgctgca tctttgcaaa acttatctaa atcaatcttt gtaagcttct ccatcttttt
     4801 acaaactctt ttaaactctc taaaatcaac actgccccat ctagtcatta tgctttatcc
     4861 ttaaataact caagtattat ttcttgatga tttggatata tagctgattc tccacttctt
     4921 acatactctt tatcatttat aataagtttt gaacctgctt taatttctat atctggagat
     4981 ataaagagtt taatagtttg ctctagctta gctaattttc cttctgtagc agaaactata
     5041 tttttatatg aaagcttgca tggttgattt tctaatacaa tcacttcttt attgttagtt
     5101 cgttttgtta cagggtcttt gattggctga tactcaacta tagtacattt atatctatat
     5161 aacatttcta ttgcttttct agttttactt accatcttaa gcacctaaag gttaatatct
     5221 tattcttacc ataagcagta agataagcta ttaagctatc aaagcgttgt tctggtgttt
     5281 gagagccact tcctatagcg aaatctacct ttgtatcacc ttctgatata gacttttcta
     5341 cagcttcaaa gttaatgctt tctatatcta attgccccat atttttcttg gtaaataaga
     5401 actctccaac tatcatatca gcttcaattt ctttcaattc aattggcata gtttttatat
     5461 tacaatctag tttaataata ttttctattt tttctcttac aaaacctatt aaccacttat
     5521 ccccatcttt taatatatat ccaaaacttt caagtctttt ttctatatca tcaattatat
     5581 tatttcccat aattttcacc tactttttag taagtttatt tttctcttta agctgcttat
     5641 tttcttcttc taaagactca acttttgacc ttaaaatatt attttcagct attaaatctt
     5701 ttacatttaa tgacttgcca taccttacta ccttaccagt ttcatctatc aaatcatatc
     5761 ccatctctaa gaaatcatct attttacact cttctatggt taatattcta tttaatttcc
     5821 ttacttgtgc cattatgctc cagct
//
LOCUS       DAGQWR010000197         5797 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.200, whole genome shotgun sequence.
ACCESSION   DAGQWR010000197 DAGQWR010000000
VERSION     DAGQWR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.200"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            218..2230
                     /locus_tag="MIW95_003588"
     CDS             218..2230
                     /locus_tag="MIW95_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3152883.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNALIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIDDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            2599..3819
                     /locus_tag="MIW95_003589"
     CDS             2599..3819
                     /locus_tag="MIW95_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY3152884.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            3856..5205
                     /locus_tag="MIW95_003590"
     CDS             3856..5205
                     /locus_tag="MIW95_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY3152885.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYSLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWFIRIISWSTL
                     AVGFATALGSFWPESATEYKGCIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFVIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     IALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(5337..5630)
                     /locus_tag="MIW95_003591"
     CDS             complement(5337..5630)
                     /locus_tag="MIW95_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152886.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
BASE COUNT         2368 a          676 c          836 g         1917 t
ORIGIN      
        1 tagaaatgaa aaatatacat acagtttaag ttttaagcaa caattagggg gatttggtga
       61 caagattttt aattataaga aagaaagtgt cttaatgcaa cttttataaa gtcattatag
      121 acactttctt ttttatttga taaatttaaa ttatcaaaat tacattgctt tatacaacta
      181 ttttgttgga aactatttat tatataagga gaaataaatg aaaaaatcag tagctctggt
      241 aaatgatagt agaaaagatt taatagactt tttagaaaat aatttgaaat tagtttttgg
      301 agattctata aatattaata gatacttcat aaatgaaata aatgataatg atattataaa
      361 tgatgatgtg atattagtaa tgtcagtaga aagattagac aaaattataa ataatatact
      421 agataagaaa aaagtgattg tggtaagaag aacttttagg gaagataaaa tttataatct
      481 actatcttta ccacagggta caaatgctct aatcgtaaat gattctgatg aaactactct
      541 agaaactata agtttatttt ataaaattgg agtgactaat attagaccaa ttccatatat
      601 gaatgataat aactataaaa atataaaaat agctataact ccaggagttc cagaaaaagt
      661 acctagcttt atctcagata tatttgattt aggtcacaga tatatagata tttctacatt
      721 tatagaaata ataaatttat tacaaattga ttcaaaggaa atacaatcta atctagttaa
      781 atactcagaa gaaataataa gtttagatac tggtataaaa gataaatata aggaattatt
      841 tttaaaaata gaagaattag atacaatatt gaatttatca aaagatggta tattatttac
      901 ttcaaaagat ggtgaaataa atacatataa tagtaaagta aaagatattt tagacataaa
      961 tgaggatata tatggaaaat acattgatga tatatttgta gatagtttaa aagttttatt
     1021 gagtgagaaa gagatactag ataaagttgt cgtattcaat aagaaatata taaatgtaaa
     1081 taaaaaaaac atatacaata gggatgaaaa aatgggcaca tattatagtt tacaagaaat
     1141 aacatacata aaaaaattag aacaaaatct cactaaaaaa ttaagagaaa aaggacaaat
     1201 agcaaaatat acatttaagg atataaaaac aaatagtcct aaaatgtttg aatgtataga
     1261 tttagctaag aaggtttcta aatctgactt aagcatactt atcagaggtg aaagtggaac
     1321 tggtaaagaa ctcatagctc agtctataca taacaactca aatagaaaaa atcaaccttt
     1381 tatagcagta aattgtgcag ccgttcctga aaatttatta gaaagccagc tattcggata
     1441 tgacaaggga acttttactg gaggtctaaa agatggaaaa caaggattat ttgaacttgc
     1501 taataatgga acaatattct tagatgaaat aggcgatatg cctttagaat tacaaacaaa
     1561 actacttaga gttcttcaag agaagcagat aatgcctgtt gggtctcaca atgtaattaa
     1621 tatagatgtt agaatcatat ctgctacaaa taagaattta gaacaaatga ttgataatag
     1681 ccaatttaga gaggatttat attatcgatt aaatacaatt ccaataaata tacctcctct
     1741 aagagaaaga aaagaagaca tattaattat tatggaagat ttaattaata agaaattagt
     1801 aatcacacca gaagcaaaaa aacttataca aaactatatg tggaaaggta atataagaga
     1861 actccaaaat gttacatcct atttaaacat tatgtgtgaa gatattgtat tagagaaaga
     1921 tttacctcca aacttaaggt cttcagataa caaaaataca tcattaaaac taaaatatag
     1981 taagaatgat atattgaata tattagagat actaatttta aataaggaat cagatgttgg
     2041 tattggtagg ggattaattt taaaagcact actcgataag aaccttcaaa ttacagaagg
     2101 aaaaattaaa aaaatatttg aatatctaaa aaaagaagaa cttataattt gtagttcagg
     2161 aagatatgga agtaaaataa ctcaaaaagg agaagatttt tacaataaat taaaatataa
     2221 aggtctataa aattataatt tatcaaacaa agaaatctat tcaggattaa agtactcctt
     2281 caataggttt ttttattttt caagtaccct aaaattataa tttattttgt acataaataa
     2341 taggaattaa taggaatagg ataatttttt tatttcctat tttttttata taaactagca
     2401 tataaaatat attttcttca aaataatatt ttttactgaa aattgaaact atgcaatcat
     2461 tttttatact atacatatag ataaatgatt atatttattc aatattctta taaaaaacaa
     2521 aattctccat cgaaaattat ggcataataa ttgcttatta tattaataaa acaataaaaa
     2581 gcaattggag gaataaaaat gcaacaggct ttgaaattaa aatatcaaac caaagaatta
     2641 ggaaaagatt ttgtaataga aagttgtaat tctataaaac catggcttat aaacataaga
     2701 agagaattac ataaaattcc agaattagct cttgaagaaa atcttactaa acagaaggta
     2761 atttcttatt taaaagaaat cggaattgat tatatggaat ttacaaaaca caatggaatt
     2821 atggcatata ttttaaaaga aagtgcagat aaaacgattt gtataagagc agatatggat
     2881 gctcttccta ttgaagaaga aaacaacata ccttataaat caatacattc tggaaaaatg
     2941 catgcttgtg gtcacgatgc acatacaacc atgctacttg gagcatgtaa agttttacat
     3001 tcaataaaag ataaacttaa tgtaaatgta aaattcttat ttcaaccagc agaagaaggt
     3061 tttggaggag caaaattttt agttgaggat gggtgtttag aaaaccccaa ggccgattat
     3121 atattcggac ttcatgttat gccacatata gaaacaggcc ttatcgaaac aaaatatgat
     3181 acattaaatg ctagtgtaga cactataaaa ataagtatca aaggtaaaag agctcatggt
     3241 gcatatcctg aaaatggtat tgatgcaata gttacagctt cacaaattgt tacatcatta
     3301 caaactataa taagtagaaa cttagaacca aataatgctg ccgttttgac tataggtaaa
     3361 atatatggtg gtgatgcaca caatgtaata tgtgaagatg taaaactaga aggcacttta
     3421 agaaccttaa atagcaaaac aagaaacttt atgattgata aaatagctaa aattgttgga
     3481 gacactgcat ctgcatttgg ttgtgttggg actttacatg taagtgatga aaactatcct
     3541 gctgttataa atgaaaaaga attagtagat acagttatat caagtactaa agaattactt
     3601 ggagaggaaa aatttatatt gagaccaaac ccgtctttag gtggtgaaga cttttctttt
     3661 tacacagaac attgtaaagg agcattcttc catttaggat gtaaaaatga ggaaaagggc
     3721 ttaatttcac ctcttcatac ctctagcttc aatatagatg aagattgttt accaataggt
     3781 gttatgatgc atgtaatgaa tactttgtat tttaattagt acagtaatta ataaaagaat
     3841 aggagataaa ttaatatgga aaaagctaag aaattaggtc tatttagtat gatattatta
     3901 gggattaata gcataatagg ttcaggtata ttcttactac caggtaaagt ttatagtcta
     3961 gcaggtcaaa acagtatgtt tatatacatt tttgctacac tcttagttct atcaatactt
     4021 ttatgttttg cagaagttgg aagcatgttt gataagaatg gaggagcata tctatactcc
     4081 aaaaaagctt ttggagattt tataggattt gaagttggta caatgtcttg gtttattaga
     4141 attatatcat ggtctactct agcagtagga tttgcaacag ctttaggttc attttggcca
     4201 gaatctgcca ctgaatacaa aggttgtata gctgcaatac tcgtaacact actttcaata
     4261 aatagcttat ttggaattaa aagcacaaag ataatgaata atgtaataac tatagctaaa
     4321 ttagtaccat taatagtttt tgtgatagtt ggaatattct ttataaaatt tgtaaatatt
     4381 gttccttcag gaaatgtagt taactctagt atgggtccag ccattatatt agtattttac
     4441 gcatttaccg gctttgaatc atttattgtg gcaagtggag aaatggaaaa tcctaaaaaa
     4501 aatcttccaa tcgcattaat aaccacaata tttatatgtg caataattta tatattaatt
     4561 caaattgtct gtatgggtat acttggagat agactatttg aaaacagtat tccaattgca
     4621 gatgcttcaa gtgtattttt aggcaattat ggtaaagttt tcatatctgt agcaacatta
     4681 atatctatat ttggtataaa tataggttca tcaatagtaa ctcctaaatg tggttcttct
     4741 ctagcagaag aaggttctct tcctgcattt ataggaaaaa caaataaata tggtgctcca
     4801 tatgtagcaa taataatatc tttaatttgt tgtataccac ttgtgctaac tggaagtttt
     4861 gaacaacttg cagtaatgag tgtaattgca agatttgctc agtacatacc aacttgttta
     4921 tcagtaatag ttttaagaaa aagaacagat gttaaggcct cttttaagat tccatttggt
     4981 cctgttatac caatggtagc tattttaggt agcttatggt tgttacaaca agcttgggca
     5041 gaagatattt caaaacctat aactcaaaat agagtcttaa taggattagg ggcaatgttg
     5101 ttaatagctc ctctatatat atttatgaaa aaaaataagg aaatcgagca taaaaataaa
     5161 tcagaagatt ctaaattata tatttcgaga gaaaaaattc aataatattt tatactatta
     5221 cttctcataa aatataataa atataaaaaa attgtagata aaactatttt ataaaattta
     5281 tctacaattt ttttatataa acatatatca aaatcaaaat attattaatt tatactttaa
     5341 agattcccat ctttatcata ttttattctt ataagaaaat ataatcctat tactactaac
     5401 tctatcacta aaaatactcc ataggtaaga attgaatcaa tactatctaa cccatttcta
     5461 aaaaaaatta atgctatacc tgctgctata atccctatag cattttttct tactgcattt
     5521 tgcccaactc cataaaaacc tggcattttt ccttttattt cgacagcagt taaaattatc
     5581 ccaaacacta taaataatac tccaataagc atcataatca tactaatcat ctaattacct
     5641 ccttcactta aggaaatttc aatttctaaa atctaaaata aataatattt attttttata
     5701 ataatattgt atatccaatt aactattttg gaaattagta ccaataaata atgtttaaat
     5761 attgaaataa tatattaatt ctaatcaata ttattaa
//
LOCUS       DAGQWR010000198         5795 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.201, whole genome shotgun sequence.
ACCESSION   DAGQWR010000198 DAGQWR010000000
VERSION     DAGQWR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.201"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            347..1621
                     /locus_tag="MIW95_003592"
     CDS             347..1621
                     /locus_tag="MIW95_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBY3152887.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSAIYVKNATEGINF
                     LAKFFKDENPDKFIITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1828..2145)
                     /gene="trxA"
                     /locus_tag="MIW95_003593"
     CDS             complement(1828..2145)
                     /gene="trxA"
                     /locus_tag="MIW95_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBY3152888.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2412..3164
                     /locus_tag="MIW95_003594"
     CDS             2412..3164
                     /locus_tag="MIW95_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBY3152889.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKN
                     KSVEYVLNIKGDKNILKKALEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHTHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3151..4032
                     /locus_tag="MIW95_003595"
     CDS             3151..4032
                     /locus_tag="MIW95_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBY3152890.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGRLNTNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNYRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4258..5613)
                     /locus_tag="MIW95_003596"
     CDS             complement(4258..5613)
                     /locus_tag="MIW95_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBY3152891.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2235 a          839 c          770 g         1951 t
ORIGIN      
        1 ttctttacta tttttacaac tttattagtc atattcataa ttatttctaa ttagtgttta
       61 acattttatg taaaatttat aaaaaaatgt atataaatta aaataagtga ctatactaaa
      121 aatatgatac ggataaacaa taagttcaac ctatctcccc caaaatcaag tgagctacag
      181 tttcccccaa ctgtagctca ttttcgtgta atttaataaa catctcaaca agaattaatt
      241 aacaaaaccc cttataaaaa cataacatat attaaacaag ctcttgatta aaacttgagc
      301 ttttttaata aaaaacagaa tagtttatta gataaggggt ttttttatgc aattcatgga
      361 tgtatctaaa tacattgaag gtgcaaacct taaagttaaa actaaaaatg gtgaaactgt
      421 taaaagaata tattttaaca actctgccac tccattagtt ttaaaaaacg tagtggataa
      481 cttaaactgt gagattcctt ggcttactta tattaatgct ccaggaataa tctcagaaaa
      541 aaatacttta aaatatgaaa atgtacgctc tactatcctc aaacttattg atggagacga
      601 agaaaaggat tctgctattt atgtcaaaaa tgctacagaa ggaataaact tcttagcaaa
      661 attttttaaa gatgaaaatc cagataaatt tattattacc acagcaatgg agcatatggc
      721 taattaccta ccttttaaag ttaattttcc tacaaaagtt gttggaatta cagaaaaagg
      781 agaacttgat ttatgtcaat tagagaatat acttaaaaat tatgctggta atgtatctct
      841 cgttacagtt acaggagcta gtaatgtcac aggtataacc actcctatat atgaaatagc
      901 aagaatagct cataagtatg gtgcaaagat attggtggat attgtgcaag taattcagca
      961 taaacctttt tcaatgatgc cacatgaatg tgatgagcat atagattttg ttgtttttag
     1021 tgctcataaa tgttattctc cactaaatgg aggagctctg gtaggaccta aaaaattctt
     1081 tgaaaaattt aatcctgtac tatatggctc aggttctacc aaatttgtaa atgataacaa
     1141 aatactatat gcaaacgtac cccaacgttt tgaagcagga tacccagagt attttggaac
     1201 tcttgcaatg ggaaaagcac tgaacacttt aaataaaatt ggtctaaaca gaatttcaaa
     1261 atacgaaaga gaattatttt tatatgctaa agaagaaatg agtaaaatac ctaatataat
     1321 aatatatgga gataaatcaa ataatgtaat tatcccaaat atatctttta atataagtaa
     1381 tatgtattac aaagatgtag caaaatattt agttgataac tttggaatag aaactggagc
     1441 tggagtagta ggtgcagata tttatgtaca aaaattatta ggtataagtc caaaagaagc
     1501 atacgtcaga tttatgaggg aaaatcctgt aggtcttgtt aggataagct taggaatgta
     1561 caatactttt gatgaggtgg atagctttat acatgctatt aaaactttgg cttttaaata
     1621 agctttttct attctttttt atcaataaat agagagtagt tttgttttca caaaaaaaca
     1681 aattatatca acagtcaatt tcattagcaa ttagacactc atttgtaact atacaaacta
     1741 ttttacacta tacaaataaa aaactcatta caatgagttt ttttgtttat atatgatatt
     1801 tattttaatt acttcaattt actttaatta catatatgat tcaattttgt tttttaaatt
     1861 ctccttaggc ataaaaccta ttaatctatc tacaggtttt ccatccttaa atattagcat
     1921 agttggtact gtagatactt caaatttctg agctaattct atactttggt ctatatccac
     1981 cttcaagaag tttgcttttt caaccatctc atttcctaaa gcttcatata ttggtgatag
     2041 catcttacaa ggtccacacc aagtcgcaaa gaaatctaca actactaaac catctttatt
     2101 ttctacttca tttataaatt cattattatt tattattttt accataatat acatctcctt
     2161 tattatcttc ttgatactaa tatactacgc cctatataac agttcaatta ggcacttttt
     2221 tgtaaccgcc tatttttaat agatttaata ttatgataaa tagatttata agattaaata
     2281 tttataagac taaatatttc tgatatattt tatttttttc ttaatatagt tgacattttc
     2341 tcccttaaat tataaaatca aattaataac tacataggta gttactaatc ttttatcaaa
     2401 ggagtttcta tatggataat ataattaatg aattacaaaa tataggattt actaaatacg
     2461 aatctcaaat atacatatca ttattaaagg aaagcccttt aacaggatat gaaataagta
     2521 aattatcagg tgtaccacaa tcaaaagtct atgaaaatat aacaaagcta ttaaacaata
     2581 atataattat caatgttgga actgacccta ttaagtacat acccataaac cctaatgaat
     2641 tattaaaaaa taagaaaaaa gaatttgata attctatgga taatctaaag aatagcttac
     2701 agatattaaa taaaaataaa agtgtagaat acgttttaaa cataaaagga gataaaaata
     2761 tattaaaaaa agcattagaa atgataaaaa atgcaaaaca agaaattata atttcttgcc
     2821 cttatgaaga aattcttgaa ttaaaaaaag aactcctaat tgcttacaaa aaaaatatta
     2881 atattgaaat actactttta gaaaacaaaa gtctgaaagg tcttgataat attcatactc
     2941 atggaggtaa taaaactaca cttgaaaata aaatgcttta caaaggaatt attttgattg
     3001 tagataatga tgaaatattg acaggaaact tatcagaagg cagtgaggcc atttcaattt
     3061 ggacaaaaaa ctctaacatt ttatatattg gagttcaata cataaaacat gaaatttata
     3121 tttcagaaca aataaaagga ggttataaaa atgaacaaaa ttaagattaa acaagttgat
     3181 gcttttacaa ctataccttt tggtggaaat cctgctggtg tcgttactga tgcctctaac
     3241 atttctgatg aaattaagca gaatgtggct aaagaaatga atttatcaga aactgcattt
     3301 gtttcagagt caaatgttgc agattttaag gtacaatttt tcacaccagg tttagaagtt
     3361 gatttatgtg gtcatgcaac aataggtact ttccatgcac ttttggaaga aggaaggctt
     3421 aatactaata agagtatatt ttatcaagaa acaaaagctg gtgtattatc tgtagagctt
     3481 aaggaaataa ataatgaaaa tatatttatg atggaacaga atacacctga atttgaaaat
     3541 ttagataatt acagaaaaga acttgcacaa atgataggtg tgcatgaaga tagacttctt
     3601 gaatatccta ttataaaggt aaatactgga ctttggtggc ttgtatttgg tctaaaatct
     3661 ttagatgatt taaaaaatat ctctccagat ttaaataaaa tcaaaaaatt tagtggtgat
     3721 aataatataa ttggtataac tccattttgt atagaaacta tagataaaaa gtgtgactac
     3781 catttaagat ctatagcacc atatgttgga gttacagaag accctgtatg tggaactggg
     3841 aacgcttgcg tatcatctta tatagtacat cacaatattt tagataaaac tagttttatt
     3901 ggtgagcaag gtaactttat aaacagacct ggaaaggtat atgtaaatat aactagagaa
     3961 aacaatgaca taacttctgt aaaaattgga ggaagtgctt atactgtttt aagtggtgaa
     4021 attatgtatt aatcccatac ctaataaaaa caaataaaaa tactagctaa ctaaagctag
     4081 tatttttatt tgttattttt atttaataat cacactctaa caaatattat aattatatat
     4141 atcataatat tgtatttaga agtaaaattt tatttttatt attaattagt tataaatcaa
     4201 tctaatacaa cataattaca gtagattaat ctataaaaca taataattac ttactgatta
     4261 tttatcatcc tcaacaagtc tacttaatgt cttatcagat aagaataata atatcgctgc
     4321 aacaaatagt attactggaa ttatagtata tactttaatc atatcaaatt taagagtaaa
     4381 tgcatatata tatccatatc ctttaccagc tataaaagta gcaaatgtcc atactcccat
     4441 aagaacacct agaagcttct taggtgcata cttacttaca aaagagttac caagtggtga
     4501 gaataacatt tcaccaagac ttaaaagaat accaaacatg attatccata gtatactagc
     4561 tttagcagtt tcaggagctc caactcctct tgaaaattct gcaccaacaa gcattaagaa
     4621 tgcaagacct aagaatatta atccaagacc tgtcttttta aataaactca tatctccttg
     4681 tggtctacta gcaagtttaa accataatgc tccaagaact ggtcctaata ctatacatac
     4741 aagtgaattt aatgagtcaa accaagcaag aggaacatca aatcctccaa cattcatgtt
     4801 tacaaatgct gcaccataat cataaactgc aagataagtt agataccaga atacccagaa
     4861 aattacagag aagaatgata taagtataat agcccaaatt ctcttcttat ccatgcttgt
     4921 aagaggagac ttatcttttt cttcaacttt ttcagcaact acaccttctt taaaaggtct
     4981 ctttcctgca tttccaagga atctccatcc atatatgaac catattgcac ctattacaca
     5041 aagtacagct gctataaaga aacattgaga aaatccaagt acaccatttt ttgcaaatgt
     5101 tttaaggtaa agaataccaa ctgctatagt accaataaaa gaacctatat taacaaaaga
     5161 atattgtact gaaaatgctg tatcaagttc ttcttgacta tcaaaaagct gtccatttac
     5221 cgcagatacg ttacctttaa agaatgcagt accaatcgaa actaagatta tcattgcatt
     5281 taccatagaa actgtagttg caatagaacc aagatagtaa cctaccccca tgataagcat
     5341 acctataggt atagtatatc tagctcctat ccatctatct gatatatatc ccccaattat
     5401 aggtgcaagg tatgtgaatg caacaaggtt ggcttgcatt atagcagctt gtcctttatc
     5461 tattccaaga ccaccatgaa ctatagctgt tgtaaggaat atatatatta agtactttga
     5521 agcataatat gctgctcttt caaatgagaa agttgttgag cagatataaa atcccatagg
     5581 aaatttaccc ttctttttta ctgttgagct cataatttta aatcctttct taattgtatt
     5641 tttatttttt acgttttgtc caaattcttt cttttatgaa agatataata ttattatttt
     5701 gtaaaacaat ttgtaattat tattaagata attgcgcatt tatttgtaga gtacatattc
     5761 tcatggaata aaattaatac acttcttaat cataa
//
LOCUS       DAGQWR010000199         5720 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.202, whole genome shotgun sequence.
ACCESSION   DAGQWR010000199 DAGQWR010000000
VERSION     DAGQWR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.202"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            127..756
                     /locus_tag="MIW95_003597"
     CDS             127..756
                     /locus_tag="MIW95_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY3152892.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(870..2384)
                     /gene="glpK"
                     /locus_tag="MIW95_003598"
     CDS             complement(870..2384)
                     /gene="glpK"
                     /locus_tag="MIW95_003598"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBY3152893.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2627..3169
                     /locus_tag="MIW95_003599"
     CDS             2627..3169
                     /locus_tag="MIW95_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY3152894.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3183..5675
                     /locus_tag="MIW95_003600"
     CDS             3183..5675
                     /locus_tag="MIW95_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3152895.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSINQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
BASE COUNT         2131 a          873 c          782 g         1934 t
ORIGIN      
        1 aaaatattat tttaaaacat gcacaatatt tgtacatatt gtagacatat aaacctataa
       61 attatacaat agtattgtta agattatata tttttttaac ataaattttg tattggggga
      121 aaaacaatgg ataaaaacta tacgatgcca atataccaaa aaatagcgtt ggatattgca
      181 aataaaatat acacgggtga aatacaagag gattcagtac tatttggacg ctctgttttg
      241 gctggtaaat acaatgtatc tccagaaacg ataagaagag ctgtcaaaat tttagaggac
      301 attggcgttg ttaaaagcat aaagggtaaa ggtgttattg tactatctcc agacaaagct
      361 tcttctttta taaaaaaata tagagatatt acaaatatat catcatataa atcaacactt
      421 tataatttaa tagatactaa atctaattta gaaaatgaaa tattagatac tataaataaa
      481 attttagact attccaacag acttgaaata atcaatcctt tagtgcccgt tcagttcacc
      541 ataaattcta attgcaaata tattggtcaa acagctgcac aaactaaatt ttggcaaaat
      601 acaggtgcta caatagttgc aattaaaaga ggtgaagaac ttataatatc tcctggacca
      661 tatattgaat ttttagaagg agacattctc ttagtagttg gtgaccaaca catttacaat
      721 tctataccaa tgtttttata tgaaaacgaa aaataaatta tataatttag tatgattata
      781 taaaaatgca aaattaacaa caaaaaaaga gcttcatatt ttacataaag ctctttttaa
      841 attcaaataa atttaacttt gattaatttt taaatatcca attcatcttc tttttcccag
      901 cttaaagctc tcgatacagc ttttttccat cctttatata atttacatct cttctcttca
      961 ctcatatttg gattaaactc tttttctact gaccactttg aagtaatttc atttcttcca
     1021 atatagaatc caacagacaa ccctgctaaa taagcagctc ctaaagctgt agtttctact
     1081 attttgggtc tatctatatc tacatttaat atatctgatt gaaattgcat taaaaaatta
     1141 ttattacttg ctccaccatc aacttttaat gattttagtt ttaattttga atcgttttgc
     1201 attgcttcta aaacatcctt tgtctgataa gcaattgatt ccaatgtggc tcttactata
     1261 tgttcttttt ttgctcctct tgtaagtcct aaaattgaac ccctagcata catatcccaa
     1321 tatggagctc caagacctgt aaatgctggc actacatata caccattagt atcctctacc
     1381 ctatttgcat acatttcact atcttttgca gattcaatta tctttaattc atctctaagc
     1441 cattgaacag atgctcctcc aataaatata cttccttcta atgcatatgt tacttttcca
     1501 tcaactcccc aagcaattgt tgtaagtaac ccatgcttag atttaactat gttactgcct
     1561 gtattcataa gtaagaaaca acccgttcca taagtatttt ttgcagtacc ttcttcaaca
     1621 caattttgac caaataatgc tgcttgttgg tctccagcac atcctgcaat aggtatttta
     1681 gctcctgata acatgcgctc atctgtatga ccataaacat agctagaagg ttttactaat
     1741 ggcaacatgc ttattggtat gtccaattct cgtaatatat cctcatccca ttctaaatca
     1801 tttatattgt acatcattgt tctagatgca tttgagtaat ctgttacatg tactttccca
     1861 cgagttaaat tccatattaa ccatgtatct atagttccaa ataataactc tccttgttct
     1921 gctttttccc tagctccttc tacattatct aatatccatt ttacttttgt tgctgaaaag
     1981 tatgcatcta ttaaaagacc tgttttttct tttattgatt cttccaatcc tttattttta
     2041 agttcatcac atatttctga tgttctccta cactgccata ctattgcgtt ataaataggt
     2101 ttaccagtat acctactcca aacaaccgta gtctctcttt gattagttat ccctattgaa
     2161 gcaatttctt ctggtcttat acctgctgtt tctataactt ctctcattac accactttgt
     2221 gacccccata tttccatagg gtcatgctct acccatccag cttttggata aatttgattg
     2281 aactcttttt gtgcaatttt cactatttcg ccttttttat taaatagtat agctcttgaa
     2341 ctggttgttc cttggtctaa tgccattaca tacttctttt ccatcaccat attctcctta
     2401 tcatataata tttaatttat taaaaagaaa aatttaatat cacatatttc aataaataat
     2461 catacttaag tacaaattta atataataga tatttataaa aagacactta ttttctatca
     2521 tatctatata ataatattat acattatagc ttacatattg attaataaat tttaaaggag
     2581 tataataaac agtgttcata ataattttaa taaaagaggt ggtaatatgc caaagatttt
     2641 agaaaatgta aaagaagata tattaaaagt atctcgggat atgattttag aagaagatta
     2701 ctcgaatatt agcataagga aaatagctca aaggtgtggt ataagtgcag gtacagtata
     2761 taattacttt aattcaaaac aagaaataat agaatatatc acaaaatctg aatgggattt
     2821 attgataagg cgaattgaat actccaataa aaacactgaa gattacataa aaaaactaaa
     2881 cattattttt actgaaataa gaaattttat aaacaaagtt cataatatcc aatacaatga
     2941 ctttttaaac acttttgaaa cagagagatt ttttgaaatg aaaaaacaca aagacgactt
     3001 tcatgaacag ctttcaaata aagtatatga agctttagaa aaagaaagtt tttttaataa
     3061 tgacaaatta gtatgcaata tcataataaa aatgtttttt tcatattcaa catatcttca
     3121 tatagaattt gaagatttaa aaccctatat tgaaaaatta atacattaag gtggtgttta
     3181 ccttgaatca taatactagt attaagaaca aggacagtaa tccaaaaata tttttagtac
     3241 ttacacttta tctattaggt atatttatgg gagctattga tactggaata gtatcaccag
     3301 caagaacgat tatacaaaat agccttggag taaatgaaaa aacgggaatt tggatgatta
     3361 ccatatacac attaacatac gcttctgtaa ttcctatctc tggaaaatta gcagataaaa
     3421 taggaagaaa atatgtgtat ctagttagta tatttctatt tggtagtggt tcattaatat
     3481 gtggattatc atcactattt tccaatttct atattttatt aattggtcgt gttattcaag
     3541 caattggtgg tggtggaatc atgccaattg ctacagcaga atttggaact accttcccag
     3601 aaaataaaag aggcatggct ttaggtttag ttggggcaac atatggtatt gccaatattt
     3661 taggttcaag cattggtagc acaattctaa gtatctttgg aactcaaaat tggaagtggc
     3721 tattttttgt aaaccttcca atttgtttaa tcatacttat tggtggagtt ttctgtatta
     3781 aaaacaataa gtctgaatct aaagaaaaaa tagataagct tggtacattg atgttgatat
     3841 gtataatcgt atcactatta tatggtctta tgaatataga cttttttaat tttaaaaatt
     3901 ctatacaaga tatatcagtt tatccatact tattgacatt tattgtactt atacctattt
     3961 tcatatttat tgaaaataga gctaaagacc ctattttaag ctttgaatat tttttaaatc
     4021 cgagaatatt gatagtactt attttatctt taattgttgg tattggaatg atgggtatgg
     4081 tattcgttcc acagtatgct gaaaatgcat taaagataaa tgctggttct ggtggatatt
     4141 ttgtagctat attgggatta tttgcaggta ttgctgctcc tttaagcgga agacttattg
     4201 acaagtttgg ggcaaagaaa atactattac taggattttc tgtatctatg ataggctctc
     4261 tttacctagt tttaattgct ctaaaaacca atactgtatt tagtgtatgt gtaagtttaa
     4321 tatttatggg attaggaatg ggctttacta tgggaactcc tctaaactat atgatgttat
     4381 caaatacaag attagaagaa tctaattcag cacttgcaac attatccttg atacgctcaa
     4441 ttggtacttc aatttctcca gctataatga ttggatttat agcacatgca ggattatctg
     4501 tgcaagataa tattatggac ttagttggaa agccctcaac tcctaaaata gttcaacttg
     4561 aagaacttga aaacatgata gatgacttaa aatcagaccc tgaaatggca aaaaatttaa
     4621 aaaatgtctc tatcccaaat atgaatgata cttcaaatat aaatatggac atgacaggtg
     4681 gcaaacttcc aaaatcctta cttaataaag ttcagtcagc agatacaacc aacattacca
     4741 atataacaaa agaaatatca acaacaatgt ttgatgaaaa agttccatct ataattacta
     4801 aaatacagaa aaatgtacaa aaaggaatag atggaacaca aagtggaata aacggtattg
     4861 aagatggtga aaataaacta aatagtggta ttaaaggagt tcaaactggt attgacaata
     4921 tggtaaaagc tagagctggt attaatcaag gaatagatgg tgtaaaaaaa ggtattgctg
     4981 gtgttgacaa aggtataaaa ggtatggaac aaggtcttga aaaacaagat gaggccatca
     5041 aggaacttac aactgcttat aataaaatac ctactacaga aaagactcct actgataacg
     5101 aaaataactc aaatgaacaa aaccagagta ataccagtaa ttctgaatca gacgacacta
     5161 agaatgattc tacagaaaat aagaataaca attccagcga gaataaacca aatgtaaatg
     5221 aacaaaaaga aagtttaaat gctcaaatac agcaactaaa aaagacaaga gatgacttaa
     5281 atacaaagtt acaaaaaaat aaaactcaaa agaacgaatt aagtaaaaaa ctaagatcaa
     5341 tggaatctca aaagaaagag ctacaaagca agttaaataa ttctataaac caaaagaaag
     5401 acatggaaaa aagccttgat acaatgcaac agcaaaaaaa atcactccaa gcagttttag
     5461 caaagacaca agaggtaaaa actgaaatac caaaggcttt tgataaatca aaattggatt
     5521 atataaattc catagaaaat aatagaacaa aaatagaaaa tacactacaa tctactctaa
     5581 actcaggatt caaacagatg tatataacag tattctgtgt aaatctacta gcatttataa
     5641 tattattgtt ctataaagaa aataaaacta gataaaagag tgttatagta ttatattgta
     5701 aatttatctt attcttaaat
//
LOCUS       DAGQWR010000200         5685 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.203, whole genome shotgun sequence.
ACCESSION   DAGQWR010000200 DAGQWR010000000
VERSION     DAGQWR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.203"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            573..1982
                     /locus_tag="MIW95_003601"
     CDS             573..1982
                     /locus_tag="MIW95_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBY3152896.1"
                     /translation="MIFSSLIFLFLFFPVTLICYYLANPKYKNVMLLLASIAFYAWGE
                     PKYIFLMILSIIVNYIFGIKVSGSKNKKMWVVLSVIYNISILAVFKYSNFFIDNINFL
                     FNTDITIPEVKLPLGISFFTFQIMSYVIDVYRNDAKVQRKLANLALYISLFPQLVAGP
                     IVRYQTVADSIEYREHNWDKFTQGVNRFVVGLGKKVILANQLGVIADGVFNKSAGDLS
                     RLEVWLGIVCYTLQIFYDFSGYSDMAIGLGKMFGFEFLENFNYPYISQTVSEFWRRWH
                     ISLGTWFKDYVYFPLGGSRVSKAKLYRNLFVVWFLTGMWHGASWTFIIWGLYYGILIS
                     VEKAFLEKFLYKMPRIVRHIYLLLIVMIGWVFFRVDDLPAAIKFIGIMFGAGNYPVIS
                     DAFNVFIIEYWYIIVGAILLSTPIVKVVKSLLIKKNKNTLNSTLYQVTNVVFIVSCMF
                     IVIIMLSSSTYNPFLYFRF"
     gene            1998..3200
                     /locus_tag="MIW95_003602"
     CDS             1998..3200
                     /locus_tag="MIW95_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152897.1"
                     /translation="MSKKKPRIVAISFLIIVFGFFILNFTLPKKKLSITENRNLAKFP
                     TVENLKKCDFIAKFEEYFNDHFAFRDEMISINTRTQANLNKTKVGNYYLGKDNWILGM
                     FPEILDQKQLSRYEKSINYLSNLSKDMGKNVYFTMMPHKTNMLKHLYPKYVDNMDNID
                     INLKNFKNRLNPDLIKYIDMDEYFLSNYSDKERENLYFKTDHHWTGYGAFEGFKMMAS
                     NMDLGLSKQDLDKHFSSYKKSIITDKKFIGSYNQNIDMLIKENEYVVYMTKDNQDYSF
                     DINGEEKEEKEEKEVYATERDNKEWDYGGAYIRGAKTNILTIKNPKALVDKKILIFRD
                     SYQAPTTLLFADMFKEVEIADPRNINSIYKTYKEIIEESNADIVMFMYNSSGFDSMID
                     SMIDKGIK"
     gene            3401..4384
                     /locus_tag="MIW95_003603"
     CDS             3401..4384
                     /locus_tag="MIW95_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152898.1"
                     /translation="MKEVFNVHIMQSQNKKIKQKYYLTKDGWLLGNFAKLLSDYHIKS
                     YSKAINNLSKLNRKKDFYFVSLPHKTNMLKHLYPSGIEGKNNIDINKENLEKQLTDIN
                     FLDIDDYFYRSFTEKEREKFFLKTDHHGNSLGAFEEYKFILDKLDLGLSKSEIDSHLK
                     RYSTRYVNDKVFIGSYNKKMGYPLKKKESISYVEISDANYKYFISNGTKNTEINENII
                     KAKLKDKREWDYGGAYMRGCDCHILKIRNSSSLTNKKALIFRDSYQAPMTWMLADTFK
                     ELEILDPRYIKNIDMTYDEIIKNSNANVVLFMYNSFGFAGMIQEMIDKGIA"
     gene            complement(4834..5277)
                     /locus_tag="MIW95_003604"
     CDS             complement(4834..5277)
                     /locus_tag="MIW95_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152899.1"
                     /translation="MKKNMLYLGIVYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GICMIFQYMYWSKPERAVDYEEKIKNQRIEMNDERRVMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(5436..>5685)
                     /locus_tag="MIW95_003605"
     CDS             complement(5436..>5685)
                     /locus_tag="MIW95_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY3152900.1"
                     /translation="WSEFIHQLKYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         2228 a          702 c          776 g         1979 t
ORIGIN      
        1 tcctaatata aaaaataaag aaatatttat aaatttaatt attagacata gataatatct
       61 aaaaaatata ccaatttcaa caaatagtaa cttttttaca acaaatggaa actatggaag
      121 caataattaa aataaaattt ataatcaatc ataattattt agtataatga ttcgatatta
      181 ttttaaagta tttgtcattt aaaacaaact tgcgataaaa gcatatctga aagtattctg
      241 attctaatac ttaataaaga gttcttttta cagttataga tataaacaac atctatctta
      301 atagactgta tagaggcaca atcaaaggta aacacatata gttaagtaat atgtccttga
      361 tttttcttta ttataatagc tttattaatt cattttaata ttacatcatt ttaaaaaaat
      421 tatatgaaaa gtcaaaattt acatatagat taattaaact tgtaaaaaca catataaatt
      481 catacaatta agtacttaaa acttgaaaat acttactttg ctatatattt tagataaaga
      541 tataattgat ttggttaaga aaggagtcta atatgatatt cagtagttta atatttttat
      601 ttttattctt tccagtcaca ctcatttgct attacttggc aaatcctaag tataaaaatg
      661 ttatgttact tctagcaagt attgcattct acgcatgggg agaaccaaaa tatatttttt
      721 tgatgatact atcaataata gtaaactata tatttggtat taaagtttct ggcagtaaaa
      781 ataaaaaaat gtgggttgtg ctttctgtta tttacaatat atctattctt gctgtattta
      841 agtatagcaa tttttttata gataatatta attttttatt caatacagat ataactatac
      901 ctgaggttaa attaccttta ggtatctcat ttttcacatt ccaaataatg agctatgtaa
      961 tagatgtata tagaaatgat gctaaagttc aaagaaaatt agctaattta gcattataca
     1021 taagtttatt ccctcagctt gtagcaggtc ctattgttag atatcagact gttgcagata
     1081 gcattgagta tagagaacac aattgggaca aatttactca aggtgtaaac agatttgttg
     1141 ttggtcttgg aaaaaaagta attctagcaa atcaattagg tgtaatcgca gatggtgtat
     1201 ttaataagtc agctggagat ttatcaagac ttgaggtatg gctaggtata gtttgctata
     1261 cacttcaaat attctatgac tttagtggct atagtgatat ggccattggt ttaggtaaga
     1321 tgtttggatt tgagttttta gaaaatttca actatccata tatctctcaa acagtatctg
     1381 agttttggag aagatggcat atatctttag gtacttggtt taaagactat gtgtacttcc
     1441 cattaggtgg aagtagagtc tctaaggcaa aactatatag aaatctattt gtagtatggt
     1501 ttttaaccgg gatgtggcat ggagctagtt ggacttttat tatatggggt ctatattatg
     1561 gaatattaat atcagtagaa aaagcattct tagaaaaatt tctatataag atgccaagaa
     1621 ttgtaagaca catatatcta cttcttatag tcatgattgg atgggtattt tttagagttg
     1681 atgatttacc agcagctatt aaatttatag gtattatgtt tggtgctgga aattatccgg
     1741 ttatcagtga tgcattcaat gtatttataa ttgaatattg gtatataatt gttggggcaa
     1801 tccttctatc aactcctatt gtaaaagttg taaaatcatt attaattaag aaaaataaaa
     1861 atacattaaa tagcacatta tatcaagtta ctaatgtggt tttcattgtt tcttgtatgt
     1921 ttattgtaat aataatgtta agtagctcaa cctataatcc attcttatat tttagattct
     1981 aattgggagg tataaacatg agtaaaaaaa aaccaaggat tgttgccatt tcatttttaa
     2041 ttatagtatt tggatttttc atactaaact tcacacttcc aaagaaaaaa ttaagtataa
     2101 ctgaaaatag aaatcttgca aaatttccga ctgtagaaaa tttaaaaaaa tgtgacttta
     2161 tagccaagtt tgaagaatat tttaatgacc attttgcatt tagagatgaa atgatatcaa
     2221 taaatacaag gactcaggct aatttaaata aaactaaggt aggtaactat tatctaggaa
     2281 aagataactg gatattaggt atgtttccag aaattttaga ccaaaaacaa ttaagtcgat
     2341 acgaaaaatc cattaattat ttatctaatc taagtaaaga tatgggaaaa aatgtgtatt
     2401 ttacaatgat gccacataaa actaatatgt taaaacattt gtatcctaaa tatgttgata
     2461 atatggataa tatagacata aatttaaaga attttaaaaa tagattaaat ccagatttaa
     2521 ttaaatatat agatatggat gagtattttt taagtaacta ttcagataaa gaaagagaaa
     2581 atttatattt taaaactgac catcattgga ctggatatgg agcatttgaa ggctttaaaa
     2641 tgatggcatc aaatatggac ttaggtctat ctaagcaaga tttagacaag cattttagta
     2701 gctataaaaa gtctataatt actgataaga aatttatagg aagttataat caaaatatag
     2761 atatgctaat taaagaaaat gaatatgtag tttatatgac aaaagataac caagattata
     2821 gctttgatat aaatggagaa gaaaaagaag aaaaagaaga aaaagaagtt tatgcaactg
     2881 aaagagataa taaagagtgg gattatggtg gtgcatacat tagaggagct aaaactaata
     2941 tattaactat aaaaaaccct aaagcattag tagataaaaa aatactaata tttagagatt
     3001 catatcaggc tcctacaaca cttttatttg ctgatatgtt taaagaagta gagatagcag
     3061 accctagaaa tataaactca atatataaaa catacaaaga gataatcgag gaaagtaatg
     3121 cagatattgt aatgtttatg tataatagtt ctgggtttga ttcaatgata gattcaatga
     3181 tagataaagg aataaagtag taaataaaaa gcctctatta aattcaaaat ttaatagagg
     3241 ctttttattg tttatttttt attgctttgg taaaaataaa ctaatcaaaa taaaagtaag
     3301 gagatgtagc atattgaaca aaagacatag tctacctata gtatcagtga tattattgat
     3361 ggtcttaatt atctttaata ataaccaaaa ttatattaca atgaaagaag tttttaatgt
     3421 ccatattatg cagagtcaaa ataaaaagat taaacaaaag tattatctaa ctaaagatgg
     3481 atggctatta ggcaactttg ctaaattatt atctgattat catataaaat cttactccaa
     3541 agccattaat aacttgagta agttaaatag aaaaaaagat ttttactttg tttcacttcc
     3601 tcataagaca aatatgctta agcacttgta tccaagtggt atagaaggta aaaataatat
     3661 agatattaac aaagaaaact tagaaaagca gttaacagac ataaatttct tagatataga
     3721 tgattacttt tatagaagtt ttaccgaaaa agagagagaa aaattctttt taaaaacaga
     3781 ccatcacgga aattcattag gagcatttga agaatataag tttattcttg ataaattaga
     3841 ccttggctta tctaaatcag aaatagatag tcatttaaaa agatacagta ctcgatatgt
     3901 aaatgacaag gtctttatag gtagttataa taaaaagatg ggatatccac ttaagaaaaa
     3961 agagtctata agctatgttg aaataagtga tgcaaattac aaatatttta ttagtaatgg
     4021 tactaaaaat actgagatta atgaaaatat cataaaagca aaattaaaag ataaaagaga
     4081 atgggactat ggtggagctt atatgagagg ttgtgattgt cacatactta agattagaaa
     4141 ttcaagctct ttaactaata aaaaggccct tatatttaga gattcgtacc aagcacctat
     4201 gacttggatg ttggctgata cttttaagga actcgaaata ctagatccta gatatataaa
     4261 aaatatagat atgacctatg atgagataat taaaaacagt aatgcgaatg tagttttatt
     4321 tatgtataat agctttggat ttgctggaat gatacaagaa atgattgaca agggaatagc
     4381 ataacaattt gaatcagctc ctttttatac aaaaaatagt tctcaataat tcctaatcta
     4441 tatgataatt tagtacatct ctttaacaat aaaaataaaa tcacaaaaat tcttatacca
     4501 tcacttatct ttatgataaa ttggttgaga aaaaatttta taatttatat gatatgcaat
     4561 cttaattaat tctttatcta ctgcaacctt taataaaaga aaccatccaa gactattaat
     4621 atgaatttat ataaaaatta ctttcaaata aagtactgca gtatttttat ttaaagtatc
     4681 tataatcttt aaccacaaaa agccacaaaa tacaatgtat acttatggct ttttataaat
     4741 ttatataatt gtatataaag atatattcta aaactgtctt aagaatctaa agtttaaaaa
     4801 tccaatattt ttattgtgaa tttaaacttt ttattacaac tttttatcta atttattgta
     4861 tacaacaaca ctacaaataa attgaaataa aataagcaat ccaagaacaa ctaaaaccca
     4921 cttgcttatt accattacag aaaatacact caatatagaa acaataaata taagaattac
     4981 aagaacatag gacatgattt gattagtgat acgtccagct ttatcacgta acataactct
     5041 tctttcatca ttcatctcta tcctttggtt ttttatcttt tcttcataat ctactgctct
     5101 ttcaggctta ctccaataca tatattgaaa aatcatacat atacctggac ctaaagcagc
     5161 tccagataat ccccaaataa aagcctcaaa tgaaaactca gtaaacgtag ccaataaaac
     5221 aagtaaaatt ccacaaataa aatatactat tccaagatat aacatattct ttttcatttt
     5281 tactcctcct cataaataaa tatttcttca atactcatct gaacctccca tgactaaagt
     5341 cacagggttc ctgcttcaca gaattttgca tactaaaata tgtcttacta attctccaca
     5401 ggccatcccc gtagttccta cggttcttac atacgttatt tattttgaat tgttattaat
     5461 cttaatcctt cttttaatat atttatactt gcatttatat ctctatcatg agtttcatta
     5521 caactaggac aaatccattc tcttatattt aagtccttaa cttcctcgtt tttataccca
     5581 catttattgc atatttgtga acttgcaaag aatttgccta cttttacaat ttgtctacca
     5641 taccaattag ccttgtattt taattgatga ataaattctg accat
//
LOCUS       DAGQWR010000201         5614 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.204, whole genome shotgun sequence.
ACCESSION   DAGQWR010000201 DAGQWR010000000
VERSION     DAGQWR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.204"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..519
                     /locus_tag="MIW95_003606"
     CDS             <1..519
                     /locus_tag="MIW95_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBY3152901.1"
                     /translation="MNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFPVGRLDKDTEG
                     LLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLDDGYKTMPSQL
                     NILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLDESLELGEYRE
                     LTDEEVKLIEER"
     gene            546..1907
                     /gene="rlmD"
                     /locus_tag="MIW95_003607"
     CDS             546..1907
                     /gene="rlmD"
                     /locus_tag="MIW95_003607"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBY3152902.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            2231..2386
                     /locus_tag="MIW95_003608"
     CDS             2231..2386
                     /locus_tag="MIW95_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152903.1"
                     /translation="MFSKNEILKKLIEEYDVKTTTDIQDMLKDLFTNTIHQIFPLSNF
                     NGLNIIY"
     gene            2430..3464
                     /locus_tag="MIW95_003609"
     CDS             2430..3464
                     /locus_tag="MIW95_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBY3152904.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPYEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKNKKRKKI"
     gene            3551..3754
                     /locus_tag="MIW95_003610"
     CDS             3551..3754
                     /locus_tag="MIW95_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBY3152905.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            3925..5085
                     /locus_tag="MIW95_003611"
     CDS             3925..5085
                     /locus_tag="MIW95_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBY3152906.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVIIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
BASE COUNT         2228 a          555 c         1076 g         1755 t
ORIGIN      
        1 atgaataaac ctgatggata tatatcagca actacagata aatatgaccc aacagtatta
       61 gatttaattg atttatcata cttagctttt gaaccatttc ctgttggaag gttagataaa
      121 gacacagagg gattattagt tttaactaat gatggaaagc tttctcatag agttttatct
      181 cctaagaagc atgtgcctaa gacttattat gcaaaaattg atggagtagt tactgaagaa
      241 gatgtagaag cttttttaga aggtgtagtg cttgatgacg gatataaaac tatgccatca
      301 cagttaaata tattaaaaag tgatgatgaa tcagaaatag agcttacaat acatgaaggt
      361 aagtttcacc aagtaaaaag aatgtttgaa agcgtaggca aaaaagtagt atacttaaaa
      421 agattgtcta tgggcaattt aaaattggat gaaagtttag agttaggtga gtatagagag
      481 ttaacagatg aagaagtaaa attgattgaa gaaagataat aaagatacta tagtaaattg
      541 gaggaatgtc tgtgaagaaa aaagatgtta tagaatttga agtagataaa atggaatttg
      601 gtggtacatc tttaagtcaa gttggagata gagttatcca tatgaagggt ggaataagtg
      661 gtcaaaaggt gagagctgga gttaaaaaag ttagaagtaa gaaagctgaa gttaagatga
      721 tagaattatt agagaactca ccacttgaaa ctgaagaaac ttgcaagcac tttagagagt
      781 gtggaggatg tacactattg tctgtgccat atgaaaaaca attagaaata aaagaaaaac
      841 aagttatgga tttattttta aagcaagatt tatttggatt tcagttttta gggatagaag
      901 gaagtccaga aaacaaatat tatagaaata agatggaata tacttttgga gatgaagtaa
      961 aaaatggacc tttgacatta ggtcttcata aaaaaggaaa gcatatagat atacaaactg
     1021 ttgaggagtg tatgttggtt gatgaagact tctcaaagat attagtttct agtgtagagt
     1081 ttttcaatga aaaaaagctt ccttattata gaacaatgaa tcacaaagga tatttgagac
     1141 atcttgtagt tagaaaaggg attcatacta atgagattat ggttaatata gtaacttctt
     1201 ctcaagaaga ttttgatatg aatgagttta aagatatgct tttagggatt gatttaaagg
     1261 ctgagttagt aggtgtactt catactatta atgatggttt agcagatgca gtccaatgtg
     1321 atgagttaag agtattgtat ggaagagatt atatacaaga agaaatttta ggtcttaagt
     1381 ttaagatttc accattttcg tttttccaaa ccaatactaa gggagctgag gttctttata
     1441 gtattgctag ggattttatt ggagattaca atgataaggt tgtatttgac ttgtatagtg
     1501 gtactgggtc tattggacag gttatggctg gtgctgctaa gaaagtgtat ggtattgaga
     1561 tagttgagga agctgttgtt gctgctaatg agaatgctaa gttgaatgga cttactaatt
     1621 gtgagtttat tgctggggat gtggctaagg ttgttaagga tttaaaggac aagcctgatt
     1681 tgattattgt tgaccctcct agaccaggca ttcataagga tgctattagg gatatttgtg
     1741 gatttggtgc taaggagatt gtttatattt cttgtaatcc taagtctttg gttgttgatt
     1801 tggttgattt taaggggtat ggttatgaga ttaagatggt taagtgtatg gatatgttcc
     1861 caaatacgcc acattgtgag actgtagtta aacttattag agaataacag ggtttcagac
     1921 ttttatattg tgtcttaaaa gtagatttgc cacctatttg ccaccataat tatttttggg
     1981 tggcaaattt attctacaat gatttcaaaa atatttacca tctggtttct catgttatta
     2041 gttacatgta aatatgtatc catagttgtt gaagttttag catgtccgag tatatgctgg
     2101 atatctttta tattagtacc attttctaat aacatagtat cagggttttt aaaataaagt
     2161 gtgtattctt ctagagtagt gataattaaa atatcaaata tcactataca aaggaaagtg
     2221 cacccttttt atgtttagca aaaacgaaat attaaaaaaa cttattgaag aatatgatgt
     2281 caaaacaact acagatattc aagatatgct aaaagattta tttacaaata caattcatca
     2341 aatatttcca ttgtcgaatt ttaacggttt aaacataata tattgactga tataataaaa
     2401 tcatagaata ttttaggggg aaatagatta tgtttaagaa aaagttaata gctgttgcaa
     2461 tgagtataac tatgattagt gtagggagct tttcgtatgc tcattcagga agaactgatt
     2521 catcaggagg tcatcgtgat aataaaaata aatctggatt aggaagttat cactatcatt
     2581 gtggtggtta tccagcccat ttacataata atggtggttg tccttatact ggaggaggaa
     2641 gcagttctgg aacaacaact tcggttaata atgaagaaaa gcaaaagagg tctgttggag
     2701 aaaaaggata taatcaaggg tatgaagatg ggtataaagg aaattatagt tcatcaaatt
     2761 atagtggaga ttatagtgat acatatgaat caaagtactc agaaggatat gaaaaaggta
     2821 aggctaaatt agaagaggaa gaaaaggtgg ctaaagagac tggatataat ttagggctta
     2881 caggtgctaa atctaataat acatatgaaa aagaagtttt aaaaaatgct tatgataccg
     2941 gatattcaac tggatacaat gaatataaga ctaaaaagat agaagaatat aaagctaaag
     3001 ggatagagga tagcaataaa gataaagaaa aaatgacatt tgaagaaaac atagattctg
     3061 aatttataga tgcttataat aatgcttatg acgaaataca agaacaatta aaaaatgatt
     3121 acactactca aggatttgag agtgctatca aaggagaaag atttgacaca tcaactatag
     3181 gtaatgttaa atatgctaat tggtttaaag aaggatatga taatggtaag gttaagttac
     3241 ctaaagcaaa agaaagtgta tataatcaag gttacaatga agaagatttt agtataccat
     3301 atgaaatgaa gtctattgaa actaagttaa aaggtgtata tgatgaaggt ttagaaaaaa
     3361 gagaagaaga gaaaagtaga aatgctactt atggggttgg agctggaaca gcagctgtag
     3421 ttgctggagt agtatataaa aataaaaaac gtaaaaagat atagatgcta gatataccaa
     3481 tgatgacaag tgtataacaa atataatttt aagtataaat atttaaaata tagaagggaa
     3541 ggttatatct atgggattta actttaggaa aagcataaat ctaggaggag gactcaaatt
     3601 aaatttaggt aagaagagtg taggtataag tgctggtgtt aaaggtgcta gagtgtcagt
     3661 taattcaaaa ggaagaaaat cagctacatt gagtatacct ggaactggaa tatcatatac
     3721 aaaaactagt acaaagaaac gtaaacctaa ataaatattt gatatactaa gaaatagact
     3781 gttacaaata gtctattttt tagtatatag atttaaaaag tgacaaaatt ttgaaatata
     3841 aactaatttc ataaaggaaa atattctttt atgtagaatt ataatattta agtaatatta
     3901 taaaatttat aggaggattt cattatgaaa ttaagcaaaa cactattggc aatagggatg
     3961 tctgtttcat tattgattgc tagttgtcca acatcaaacg ctcttagttc tattaattca
     4021 ataaaaggta caaataagta tgaaacagca ggtttaatag cagataaaca aaattatgat
     4081 attgcaatac ttattaattc tgaaaatgga ttatctgatg gattgtcagc aagtggattg
     4141 tcaggagcta aaaatgcccc aatattgtta actaagaaag atacaatccc ttcagaaaca
     4201 ttaaaaagat tagaaaaggt aaaagaagta tatctaattg gaggaaaaaa ttcgatagga
     4261 gcatcagttc agaaaatatt aagtgataaa aaaataagca taaatagaat aagtggaact
     4321 gatagaatag atacaagtca taaagttgca aaagaaatag gaagagtaag tacggttaaa
     4381 aaagtaatat taactaatgc ttttaaaggc gaaccagatg ctatgagtgt tgcttctata
     4441 gcagttagag ataaatctcc aatagtatta actgatggaa atactacaag ttttgctaca
     4501 gatggattag aaacttatgc aattggtgga aaagtcagta tgagtgataa gttaataaat
     4561 gaaactaaag caacaagaat aggtggagta gatagatacg atacaaataa acaaataata
     4621 aataaatttt ataatggctc aaaagaattt tacatagcaa gtgggactga tttagtttat
     4681 tctttagttg gttctacaat tgcacaaaaa acacctatag tgttagttgg aaatggaagt
     4741 aataagagta tattaaaaaa tgcaagtaaa ataactgcta ttggaaatat tgatgatgtt
     4801 attataaaac aatgtttaaa tataactaat aatgttgggg attcaaatac aggtattgta
     4861 aaagatacta attcaaataa tacaaacaaa ccaagtaatg agcctagctc taataatcct
     4921 ggattaactg ataaaagtac agtatatgta actcctaatg gaaaatcata tcatctaaca
     4981 aaaaattgtt ctacactaaa aagaagtaaa acaataaata aagtaacatt aaaacaagct
     5041 aagtcacaag gtaaaataga cccttgtaat gtttgtgtaa aataatgtat tagccaaata
     5101 tatattaaaa aagacaatta attaagattg tcttttttat tgaagaaata taaatttata
     5161 ttatgtatta ttaaaaagaa aattattaat tataaaaaat aataataaag acgataagta
     5221 ttaaaatttt atgtttccaa tatttatgat ataattaata tggcaaggaa taaatatttg
     5281 aaaaagctat gagtggtgtt tccatggaag tttcttcact ttcttatgag aggaggtgaa
     5341 aactatggaa aatctaatga tgagtattat aactggtgtt atagccagtt acatttacga
     5401 taaattaaag agccactctg accaatgaac tatacccact gttttataca gttaaaacat
     5461 gtaaaagtgg ttataaagta ctagtgtcaa tcattagtac tttttttatt atattcatat
     5521 aattaatagc tatattttaa agtttactat ctaaaaaata gcacgtcaaa taaaacccta
     5581 gatatttaaa tttttttaga gctacttaaa ataa
//
LOCUS       DAGQWR010000202         5609 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.205, whole genome shotgun sequence.
ACCESSION   DAGQWR010000202 DAGQWR010000000
VERSION     DAGQWR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.205"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..1215
                     /locus_tag="MIW95_003612"
     CDS             <1..1215
                     /locus_tag="MIW95_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152907.1"
                     /translation="VNKVRNLVLEAKNVTIEDTENNFTSDNVEGALKEVFQNGVNAKS
                     NVVTALNSKGADVTTSDTWEEIKNKIDIKEGRLDLRETTLSNNYSYLVTNGAIKYIER
                     CSGNFKTFEYEEPYFYVIKETHLIKINAIDETVVFDITLANANFSCICVTQEYLFISD
                     NTKLYKINKITGIEVQSIEGAYYKLCTYGEFIYGVYGDETSSTLHKIRISDMYIMLTK
                     NLVSNGIYNFKGGKFVCNKNAIYATTEHSNSSSITTCHLSKINFDFAIAKDFRIGGYL
                     HMKNIKFLNDFVIVSDAERGIEIDSNKKSALVKYDANLNLIAYSDDSKYDNFDIYNGY
                     IYAIYSPSSSPFVKISLNTLKHIDSYRKLTETYPNAGMFVINDIVFFISSGIFRNILS
                     KKVYSDEKGESL"
     gene            1212..1511
                     /locus_tag="MIW95_003613"
     CDS             1212..1511
                     /locus_tag="MIW95_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152908.1"
                     /translation="MIYLGNLMDTEEQNIKYVGMIHYEPNLLSEENLKQGILIESLPT
                     QKYAENKEAKLFINIDTKEVFYRYTDIKSNIEDKVNSTEQTIADLTFQLMSNGVI"
     gene            1513..1650
                     /locus_tag="MIW95_003614"
     CDS             1513..1650
                     /locus_tag="MIW95_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBY3152909.1"
                     /translation="MNWYKIIADFYNNGNWTKEQVKTAVEKNKITASEYKEITGEDYI
                     A"
     gene            1682..1912
                     /locus_tag="MIW95_003615"
     CDS             1682..1912
                     /locus_tag="MIW95_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBY3152910.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAVQRGIF"
     gene            1931..2188
                     /locus_tag="MIW95_003616"
     CDS             1931..2188
                     /locus_tag="MIW95_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBY3152911.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLSPVAVLQGVICWGISIGINQTYKQLKEENK"
     gene            2188..3000
                     /locus_tag="MIW95_003617"
     CDS             2188..3000
                     /locus_tag="MIW95_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY3152912.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKVDVIICPEKQFKTKSEEKSYKIPRVNAGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICKKLGTIFKNRGAKLDKGLYILNSSKPTAILIESFFCDNKDDYEKAKKF
                     GYEGMAKLIVEGVLNKNIGNDGVKLMYKHTIVYDGEVDKIPATVVGWGYNDGKILICD
                     IKDYIPGQTENLYIVGGGACNKIGSITKEKYTMIKGNDRFDTLYKALDFIDR"
     gene            3250..3741
                     /locus_tag="MIW95_003618"
     CDS             3250..3741
                     /locus_tag="MIW95_003618"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152913.1"
                     /translation="MAYENGTDFIDYFPDCIEKYKEDNKIFEKAILLLNLYMQKPTWP
                     HCVNEYKSQAEKILGEDPEFIGKYGIQLRKFYDEDENAKVSKEFKNDLYNFSKSTYDI
                     LDNVYFSKSHPFNLSSIYSTSRDDDFIIKIFRYDNNFLELEMSKREVESLINYLSDLL
                     KEE"
     gene            3743..4309
                     /locus_tag="MIW95_003619"
     CDS             3743..4309
                     /locus_tag="MIW95_003619"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152914.1"
                     /translation="MLGAKKEKEFNLLSFNEKKIEKHKCKNYHNDSSKDKYKYSQSEF
                     IAATLKEADDMNEEERRILEAEKRILETEKRVHQDNKEVKESIDKQFNDIKGIFKEYK
                     NDLDKDRIELKNDLARERAELKSDLKEAINEHKKVTEKDVSEIKNSMKGIEDRIDSTN
                     KWIIGLCITTIIGIATMAITIGISIWPK"
     gene            complement(4719..4907)
                     /locus_tag="MIW95_003620"
     CDS             complement(4719..4907)
                     /locus_tag="MIW95_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152915.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHDYSKK"
     gene            5165..5308
                     /locus_tag="MIW95_003621"
     CDS             5165..5308
                     /locus_tag="MIW95_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152916.1"
                     /translation="MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNK
                     KRH"
BASE COUNT         2321 a          563 c          912 g         1813 t
ORIGIN      
        1 gttaataaag ttagaaatct tgtattagaa gcaaagaatg taactataga agatacagaa
       61 aataacttta caagtgataa tgtagaagga gcattgaaag aggtttttca aaatggagtt
      121 aatgctaaaa gtaatgtagt aacagcatta aactccaaag gtgcagatgt tactacaagc
      181 gatacatggg aagaaataaa gaataaaatt gatataaaag aggggagatt agatttaaga
      241 gaaacaacac tttcaaataa ctattcgtat ttagttacaa atggagctat aaaatatatt
      301 gaaagatgta gtgggaactt caaaactttt gaatatgaag aaccatattt ttatgtaatt
      361 aaagaaactc atctaattaa aattaatgct attgatgaaa cagtagtttt tgacattact
      421 ttagctaatg ctaacttctc atgtatctgt gttactcaag agtatttatt tatatctgac
      481 aatactaaat tatataaaat aaacaagata acaggaattg aagtgcagtc aatagaaggt
      541 gcttattata agttatgtac ttatggggaa tttatttatg gagtatatgg agatgagact
      601 tcttctacac ttcataaaat tagaatatct gatatgtata taatgctaac taaaaattta
      661 gtttctaatg gtatttacaa ttttaaagga ggtaagtttg tttgcaataa gaatgctatt
      721 tatgctacaa cagaacactc aaattcaagt agtattacaa catgtcattt atctaaaata
      781 aattttgatt ttgccattgc taaagatttt agaattggag gatatttgca tatgaaaaac
      841 attaagtttt taaatgattt tgttattgta tctgatgcag aaagaggtat agaaattgat
      901 agcaacaaaa aaagtgcttt agtaaaatat gacgcaaatt taaatttaat tgcgtattca
      961 gacgatagca aatatgacaa ttttgatata tataatggat atatatatgc tatatattcg
     1021 ccttctagta gtccttttgt aaaaataagt ttaaatactc ttaaacatat cgacagctac
     1081 cgaaaactta ctgaaacata cccaaatgct ggtatgtttg taataaatga tatagttttc
     1141 tttattagta gtggaatttt tagaaatata ttgtcaaaaa aggtttattc ggatgagaaa
     1201 ggagaatcat tatgatttat ttaggaaatt taatggatac agaagaacaa aatataaaat
     1261 atgttggtat gatacactat gaaccaaatt tgttatcgga ggaaaactta aaacaaggta
     1321 ttttgataga gagtttacca actcaaaagt atgcagagaa taaagaagca aagctattta
     1381 taaatataga tactaaagag gttttctata gatatacaga tattaaaagt aacatagaag
     1441 ataaagtaaa ttctacagaa caaacaatag cagatttaac atttcaatta atgagtaatg
     1501 gggtgatata gtatgaattg gtataagata atagcagatt tctataataa tggtaattgg
     1561 actaaagagc aagttaaaac agcagtagaa aagaataaga taacagcaag tgaatataaa
     1621 gaaataacag gagaggacta tatagcatag tcttttttaa ttcaaaaatt aggaggtttt
     1681 catgaatgaa gaacttttcg aagcagattt aaaaagacat gaaacaagaa taaataaaca
     1741 tggagaagaa atagacgaat taaaaatagc aaatatagag tctaaagcag agttaaaagc
     1801 attgtgtgag aatttaaact cacttacaag tatgctcaaa tggctaattg gaacaatgat
     1861 tacaacacta gtagggttct ttatatttgc agttcaaaga ggaatatttt aattaggagg
     1921 aaaataaaag atggataatt taataagttt cataccagag cagttgctaa ttttagtagc
     1981 tgctctctct attataggta agggctgtaa gaagtataag caactagata acaaatatat
     2041 tccagtagtg ttattaatac ttggtatagg attttctatt tggatgttag gactaagtcc
     2101 tgttgcagtc ttacaaggcg tgatttgttg gggtatatca ataggtataa accaaactta
     2161 caaacagttg aaggaggaaa ataaataatg aaaatatgta taacagtagg acacagtatt
     2221 ttaaaaagtg gagcatgtac ttctgctgat ggagtagtta acgagtatca atacaacaaa
     2281 tctcttgcac cagtattagc agatacattt agaaaagaag ggcataaggt agatgtaata
     2341 atatgcccag aaaagcagtt taaaactaag agtgaggaaa agtcttataa aatacctaga
     2401 gttaatgctg gaggatatga cttactcata gaactacatt taaatgcaag tgatgggcaa
     2461 ggaaaaggct cagaggttct atattacagt aataaaggtc tagagtatgc aactagaata
     2521 tgtaagaaac taggtacaat atttaaaaac agaggtgcta aattagataa aggattatat
     2581 attttaaata gttcaaaacc tactgcaata ctaattgaaa gtttcttttg tgacaataaa
     2641 gatgattatg agaaggctaa gaaatttgga tatgaaggta tggctaagtt aattgttgaa
     2701 ggtgtattga ataagaatat aggaaatgat ggagttaaac tgatgtacaa acatacaatc
     2761 gtttatgatg gagaagttga caaaatccct gcaactgtag ttggctgggg ttataatgat
     2821 ggaaaaatac tgatatgtga tataaaagat tatataccag gtcagacaga aaatttatat
     2881 attgtgggtg gaggagcatg taataagata ggttctataa ccaaagaaaa atacacaatg
     2941 ataaagggta atgatagatt tgatacactt tacaaagcat tagattttat tgatagataa
     3001 aaggagtata gttaatgtat tttacatttt gcttatactt tatgccaata ttacgacaat
     3061 ttataaaata aaaaatgata tattaaagat aatcataagc cattttaaaa tccaaaaaac
     3121 taggtgtata aacagttaac aaatatatat attgataaaa taatggaggt gaaataaaaa
     3181 atagtagtgc aataacaata attctaatgt agaataatat taaataatat tattttaggg
     3241 ggatttaaaa tggcttatga aaatggaact gattttatag attattttcc tgattgtata
     3301 gaaaaatata aggaagataa taaaatcttt gagaaggcaa tattattact caatctgtat
     3361 atgcaaaaac caacatggcc acattgtgtt aatgagtaca agagccaggc ggaaaaaatt
     3421 ttaggtgaag acccagagtt tataggaaag tatggtatac aattaagaaa attttatgat
     3481 gaagatgaaa atgcaaaagt aagtaaggaa tttaaaaatg atttgtataa tttttctaag
     3541 agtacatatg atatactaga taatgtttat ttttcaaaaa gtcatccttt taatctttca
     3601 tctatttatt caacttctag agatgatgat tttataataa aaatatttag atatgataat
     3661 aattttcttg aattagagat gtcaaagaga gaagtagagt cattaataaa ctatttatct
     3721 gatttattaa aggaagaata gtatgttagg agcaaaaaaa gaaaaagagt ttaatttatt
     3781 gagttttaat gaaaaaaaga tagaaaaaca taaatgtaaa aattatcata atgattcttc
     3841 gaaagataaa tacaaatata gtcaaagtga atttatagct gctactttaa aggaggcaga
     3901 tgatatgaat gaagaagaaa gaagaatact agaagcagaa aaaagaatat tagagactga
     3961 gaaaagagtg catcaagata acaaggaagt aaaagaaagt atagataagc aatttaatga
     4021 cataaagggt atttttaaag aatacaagaa tgatttagac aaagatagaa tagaactaaa
     4081 aaatgattta gctagagaaa gagctgagtt aaaaagtgat ttaaaagaag ctataaacga
     4141 acataaaaag gtcacagaaa aggatgtaag tgaaataaag aatagtatga aaggtataga
     4201 agatagaatt gattcaacta ataaatggat tattggacta tgtataacta ctataatagg
     4261 aattgctaca atggctatta caataggtat atctatatgg ccaaagtaac acaaaaagag
     4321 gtaactagaa ttaaaatagt tatctctttt tatacttaaa caaaacattg aaaaaatagt
     4381 aaaaaatatt aacaggatac ataaaactat agtataacct gtaataataa aacgaacgaa
     4441 aattatcata ctaaaacata agacatgcta taattgtatt agataaatgc ttgaatatat
     4501 accaaaagta ctctttttat aagagtgctt atttttttga aattcatcaa catataaact
     4561 atcaagaaca ttactcaaca caccttaaaa ttgatttaaa ttctttttca tacacaaagt
     4621 tatatgatat aataaaaaag taaatatgta accccaacac atctttacta agtcaaacat
     4681 tattatatag agcattcttc attatggaga gtgctttttc atttctttga ataatcatgt
     4741 tgattattta taatattttc atttaattta tctttatcta tcaaatttaa tgcacaatta
     4801 atacaaatgt tagttttcat atgacttttc ttatgaaaag taatatactt attatcatct
     4861 ttatttattc ctttattaca gtaatcacat aatatagtct tagtcatatt tttattcctt
     4921 tcattttata ttttccataa ttaagtttaa catatttagt atatacctat gtatatatat
     4981 ttaaaacaat gttttttatt gcaatataga atatattgta taaataaaaa aaatggagaa
     5041 tagatatttg ataattgtaa aattatgtta taattaaaat gcaagaataa tcttgtggaa
     5101 ctacaatcta agagtggagc ttcattttct acattccatc ccttaaaagg aaggaggtgg
     5161 agaaatgagc gaatttttac taggagtgtt agctagttta acagctagct ttattacata
     5221 tattatttcc agaaaagtaa aaagccactc tggcaggagt gactttgagc ttgatgtaaa
     5281 aatcaagttt aataaaaaac gacattaata tttagttaat gaaacttcac tctaggctaa
     5341 tagattgtag tttctttttt tgtttttgat acgaatttac atctttatta tatcgcattt
     5401 tgagaaaaaa taaaactatg aatattagaa atatatttta ttttatctct aagtatatat
     5461 attattagcc acctaaaaaa ctttcacaaa aacaaaaaga gtatatgttt ttgataactg
     5521 gatgatataa cttataactc attttacaat tcttagttca tatatattta tgccactaaa
     5581 tttctctagt tttgccgatt tagttttat
//
LOCUS       DAGQWR010000203         5587 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.206, whole genome shotgun sequence.
ACCESSION   DAGQWR010000203 DAGQWR010000000
VERSION     DAGQWR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.206"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            301..522
                     /locus_tag="MIW95_003622"
     CDS             301..522
                     /locus_tag="MIW95_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152917.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(811..1575)
                     /locus_tag="MIW95_003623"
     CDS             complement(811..1575)
                     /locus_tag="MIW95_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBY3152918.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKAPTPDIVDILSEFNVNVL"
     gene            1849..2523
                     /gene="fsa"
                     /locus_tag="MIW95_003624"
     CDS             1849..2523
                     /gene="fsa"
                     /locus_tag="MIW95_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBY3152919.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILDEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(2645..4039)
                     /locus_tag="MIW95_003625"
     CDS             complement(2645..4039)
                     /locus_tag="MIW95_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBY3152920.1"
                     /translation="MGIILKPKQFYMTVIVTIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(4117..5463)
                     /locus_tag="MIW95_003626"
     CDS             complement(4117..5463)
                     /locus_tag="MIW95_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY3152921.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGIQQMLFSIGNMTLQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGYWKTKAIVNHRINE"
BASE COUNT         2226 a          734 c          735 g         1892 t
ORIGIN      
        1 aaccataaaa tacaccaact ataatgatga gatttcctta catcctccat ataagtcatc
       61 attatagtaa cccaacaatt aagttactat gaatttaaaa aataacaccg agcatataat
      121 ctatgcttag tgttattttt attttgaaat aaaaaatatt taatttattg aaatgtatag
      181 atttagacag tttaaactat atttaagact taaaaattat aattgatatt acaaataaat
      241 atatgtactt tgggaaaaat aaatatagga agtattatat ttacagagga ggtagttttg
      301 atggatagat taaaaagaga tatgaaaaat atgaaagaag atttaaaaga aggtgttaaa
      361 aagacagctt atgttatgaa aaatacagct gaagatttga aagatgatgc acaaggtgct
      421 atggtaaact tagaaatgaa aaaagatgag atgatggaaa gatatgaaca aaagaaattt
      481 tctaaagaat tagagaagca aatgaagaat gaaatgaaat aagcttttat ttatgaataa
      541 caaattattg tattcatata aaaagtagcc agtaatatta aatagtttat ttaaggaaat
      601 agactttcta aaaataactg tattagattt agaaagtcta tttcaaatta gttttataaa
      661 tagacataag gataaagtag atgttataga actagatttt aaaaaataga tactaaggat
      721 aaaataaata ttatagaaat atgtttttaa gaatagttat caaataaaaa tttatattta
      781 taagaaatcc tataaaatat ataattaaac ttataaaaca tttacattaa attctgataa
      841 aatatctact atatcaggag taggcgcttt ttctgtaatt atagaactaa tatcttctaa
      901 atgagcaaac ttgaaatttc catcaaattt aaacttttta tcttccataa ctataaatac
      961 ttttttactt gaatttatta tagccttctt cgtgagacca tcttcaattt caaaagttgt
     1021 aagtgaagag ttgaatacat ctactccaca acttcccata aaagtcttat caaacttata
     1081 tttcgatata aactctatag ttggagtacc gacaaaaccg tctaaactga gatttaataa
     1141 acctccagtg gaaattatat ttaaattatt tggttttgaa gtaactacat ttagtatatc
     1201 caacatattt gtaacaagtg ttactttttt attactttca gctaacaact ttgcaagcaa
     1261 gatgttaatt gtagagatat ctaaaaatat agtttctcta tcttcaatta aattaaatgc
     1321 tttttctgct attattttct ttgttggaat attaatttct tttctgtctt ttgtatcctg
     1381 attctctagt gtttctctag ctagtacagc tcccccataa attcttctta ttgaagaaat
     1441 cttttctata ttttttaaat cttttcttat acaatcttct gtaacattaa atttagaact
     1501 gagctctttg acagtaactc taccttcttt ttttaggata tttaatatag cttggattct
     1561 ttcttctgca aacattttct acctcttttc gcaatatagt attatagtaa atattataag
     1621 ataaaataca atatatcaac tattttaaca tgaaatacaa aattaattta atataaaaag
     1681 tatgtttttt ctaactatat atttagaatt tagtaaataa aaaaattata aaactaagaa
     1741 ggaaaagcaa aacaataata aaaaataaaa aacaataagc aaaatgttga aaataatacc
     1801 aaatcaatat ataataagaa taaagaaaga ttaaaggggg atattttaat gcaactaatc
     1861 ttagatacag gtaatgtgga ggagattaag gagttatgta cttgtttacc aatagatggt
     1921 gtaactacaa atccaagtat tgtttcaaaa gaaaaaaaga attttaagca acttataaat
     1981 gaaattggtg agattatagg tgaggatatg cctatacatg ctcaagtgtt aagtacaaag
     2041 tatgaagaaa tattagacga agcattatat ataagtagtt tgagaaaaaa tatatatgta
     2101 aaaatacctg taacacaaga tggtttaaga gctataaaag accttcacag aaaaggtgta
     2161 aaaataactg ctactgcaat attcactgct catcaaggtt ttttagctgc aaaggctgga
     2221 gcaaattata ttgcacctta tgtaaatagg cttgataata tatctggtga tggagtagct
     2281 atggtttcag aacttataaa aataatagat acttataaaa tggatacaaa agtacttgca
     2341 gccagtttta aaaatgcaca acaagttata gaattaatgc aacatggggt gcatagtgtt
     2401 acagtaccat atgatatttg taaatctatg atgaatcatc cattaactga ttggagtgta
     2461 gataagttta tagaggattg ggagaatact tttggaaaag gaagtaaaac aaataatatt
     2521 tagtattatg gtaaaagagt agttttgtcc aatttcacaa gaacttatat aaaaagatga
     2581 aaatatgaat tttcttttta ataatttcgt tattatctta agttaattca tattttcatc
     2641 ttttttagat ttctagtcct ataaatttcc accaagggaa tgttatacat attattataa
     2701 agtaggttaa aggaatcaaa gttaaaccgt atttgaaaat aaccttgcta ttatacagat
     2761 tttcaccatg accaaccatt attgtagcat gatgaaatgg taatatgtac tgtatgctta
     2821 ctataatgta gacaaataga cataaagtaa cactattttc ctttagtata tgtaaacttc
     2881 caagtaatgg tattactaca gatgatgtag tgacagcact tccaagacat atattcaaga
     2941 ttattgtgag agttatcaaa aacaatattg aaataatttc tgaagagttt ggtataaact
     3001 ttatcaaata atttcccatt atatctacaa ctccagagta ctttaacact cctccaattg
     3061 aaaatgctgc tataaaaaaa ataaggacct tccaatttat ttcttttaaa gtactaaatc
     3121 caactatttt tcttagtaat aaaagaagta cacaaattga cattataatt aatgtgttta
     3181 tgttatgtat gaactgagtt aaaaacataa aaaacattat tcctaggcat aaaaatacgt
     3241 gtttttgctc tttgtttagc aaattaaatt ctttttttaa attagaatat gaaaaatgaa
     3301 gtttattacc ctcaagattt tcattagaaa taaattttat tgaatgcaat tcttttttaa
     3361 agacaaagat aaataaaaaa taagttataa aacaagtaat tatagttgga acagacatat
     3421 aaaaagccca ttgaccccaa tttatattac aatttccaag ttctagtaca acataattta
     3481 gaagggtgtc tccatttaca aaaaacattg atgtaaaggt agatgctgta aatatactaa
     3541 ataaaagtat agattttgaa tcttcagtta tctgttgttc ttttaaaaac tccttgtaaa
     3601 aagcagatac aagaataacc cttggaaatg gttgtgggat aaaaaatatt aaaagaatac
     3661 ctaaaatata cgataaaaga atcatctttt taggggtatt tacagtattt aaaattaact
     3721 tctcagaaat aatttttgcg acacctgttt ttgtaactgc attagtaatt atgtatgcaa
     3781 gaattattat gtaaaaattt tctgtaaata aaaactgtaa aataaccttt attggtgcaa
     3841 tgttaaatat aaacgctaga attacaaaaa acaatgcaac aaaagttttt tcaactgagt
     3901 ctacagccca tatcaagata gttaaaaaag ataagcatat agaaaatttt atgttgaaac
     3961 tgtaattaag agagaagtag agaattatgg taactataac agtcatataa aattgttttg
     4021 gcttaagaat aatacccatt tttatattca cctccataat ttattatatc ataatggagg
     4081 tgaatacaat gaatgtgaat acattttata aaaagattat tcattgattc tatgatttac
     4141 aatagcttta gttttccaat accctttctt atagtagata actgtaacaa taagacctaa
     4201 agtccaacca aaaggaatac ccatccaaat tccgtctgtt ccaaaaaatt tagaaagcat
     4261 ataagaagct ggaagtcttc caagccataa agatactatt gagcttataa gtgcaaacat
     4321 ggaatcacca gcacctttca ttgcacttga aagtacaaaa gaagttccaa taaataaaaa
     4381 gaatggtcct attataaata agtaagaaga gccaatttta atgacatctt tatcagtatt
     4441 gaatagagca attagatttt ccctaaatag caaaattaaa gcaataactg taactgctaa
     4501 aactaaggtc atttttatag attctcgaat gcctttttta accctttcat tttctccagc
     4561 acctatgttt tgagcaacaa aagtagaaac agcagaacca agattcataa taggtaagct
     4621 tataaaattt tctatcctaa gaccagcacc aaaagctgcc atagcagatg taccataact
     4681 attaactaaa aattgaagag tcatatttcc aattgaaaac aacatttgtt gaattccact
     4741 tggaagtcct aatctaagtc catctttaaa tattttgtta tcgtatacaa ttcctttgat
     4801 tttaaattta agcacttcat gttttttatt taaataaatt atacttatta taaatgatac
     4861 tgcctgagcc attatagtgg caagtgcaac gccctcaaca cccattctta gcactacaat
     4921 aaaagttaag tctaaaacta tatttagaat tgtggctatt atcagaaaat aaagtggagt
     4981 tactgaatct ccaagtcctc taagtattgc acttactgaa ttatatccag cagaaaataa
     5041 tattccacta aatattatag taagatacaa ttttgattga gcaaaaacgg attctggtgt
     5101 attcataagc ttaagcattg ggccaccaaa tactattcca agaatactta ttaatataga
     5161 accaataaat aaaagtatat atgtagtatc aatagtagct tttacctttt ttaaatctcc
     5221 tgagccataa aattgtgaca ctaaaataga aaatccaagg ctaacaccca taagcgctgc
     5281 caccaataaa aacattatag ggttagctcc agagactgcc gccattgctt cttttccgat
     5341 aaatctacca actattatac tgtcagctgt attgtatagt tgttgaaata aactaccaat
     5401 aagcataggc atggcaaaaa agaatatgga ttttccctca tgacctgtag ttaaatcttt
     5461 caccttattc ctccaaataa aaaaattatt aatttattat agtataatct taacataaac
     5521 tttatatcca aactataaat aaattgtatc aaaatatcat atattttttt aaaatgtaaa
     5581 gaatata
//
LOCUS       DAGQWR010000204         5575 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.207, whole genome shotgun sequence.
ACCESSION   DAGQWR010000204 DAGQWR010000000
VERSION     DAGQWR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.207"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            58..1239
                     /locus_tag="MIW95_003627"
     CDS             58..1239
                     /locus_tag="MIW95_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBY3152922.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQVDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1381..2298)
                     /locus_tag="MIW95_003628"
     CDS             complement(1381..2298)
                     /locus_tag="MIW95_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152923.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2551..3345
                     /locus_tag="MIW95_003629"
     CDS             2551..3345
                     /locus_tag="MIW95_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152924.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3346..5406
                     /locus_tag="MIW95_003630"
     CDS             3346..5406
                     /locus_tag="MIW95_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY3152925.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFACYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEELFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2133 a          631 c          914 g         1897 t
ORIGIN      
        1 aatattagaa ataataaata aataatacat tgtacttaaa agggggagaa atttaagatg
       61 aacaaaaaat atattaaagc agggaattta attgatggaa caggaaatag tattatgcaa
      121 aataagggta taattattga tggagatact atagttgaaa ttgaagatat aagagatgat
      181 ttaaatggat atgatgtgta cgattactta gataaaactg ttatgccagg cattatgaat
      241 tgtcatgttc acttgacaat ggagccagtt ggaaatccaa agatatatta tgataatgta
      301 tcagatgtag agcttgtagt aaatacgata aaacaagttg atgcttattt ggattcagga
      361 gtaacatata ttagaagctt aggttgtccc aaatatgtgg atgtacaact taaaaatctt
      421 atagacaaag ggtctataga tggtcctggt atagttactt caggtccagt tatatgtatg
      481 acaggagggc atggatatta ttttggtatt gaaagtgatg gagtggatga atgtagaaaa
      541 tcagccagaa cagttttaaa aaatggagta gattgtgtaa agataatggc aactggaggt
      601 gtaacaactg atggagttga gccagggtca ccacaattaa ctttggagga aatgaaggct
      661 gctgtagatg aagctataaa agcaggtaaa ataactgcca cacatgcaca aggaagaact
      721 ggtataaaaa atgcagttct tgcaggaatt acttctatag aacatggtgt ttatttagat
      781 gatgaaataa ttgatttgat gcttgaaaga ggaacttact tagttccaac tgtggctgca
      841 ccatatttta ttctaaatgc aggaaaagaa agtggaataa ctgaagctac tcttagaaag
      901 tgtgagattg ttgccattcc acataaagaa tcattcctaa aagcatataa aaaaggaatt
      961 aaaattgcag ttggttctga tgcaggaact tcttataatg aacatggaaa aacatattat
     1021 gaaatgaagc taatgtcaga ttatggtatg gataatatgg atataatagt tgctgctaca
     1081 aaaacagcat ctgaactttt aagaattgat aaaaattatg gaactctaga aaaaggaaag
     1141 aaagcagatg ttatagttgt aaatggaaat ccagtagaga atatagacgt acttgcagat
     1201 gtgttagcag tatttaaact tggtaaaaaa gttagataaa tagtataaaa ataaaaaaga
     1261 gtgtctaaag aaatttttaa ataattatag acactctttt ttattagtaa taattaaatc
     1321 ttttatactt tagaaaatag attctatttt atatttagat ataactcttt aaaatcattc
     1381 ttatgcaata taatctatag aatctacaca tacaaagtat attcttgaat cattagaatt
     1441 tccaagtact ataacatcat ttttcttacc tagaacttca acaccagtta gaaccaaact
     1501 accagctact acagtaactt gattatttat atttgacata taaagattat ctaggatacc
     1561 tttattacaa cagcaatcat cacaatcgca gttacaatta cattctttgt aatgtttttt
     1621 atcaagatag taacctaaag atgctatgaa atcttcataa tctgcaggtg taatatcaaa
     1681 tgcaacagct ttaagattac acaatgatgc taaatctacg tttgctaatg gagtagatgc
     1741 agcaagtata aaatcaatta tagcagcaag tattgcatct aatatagccc caaggtctat
     1801 cgtaggagga ataactgcat caataagtgc aattaattca gctactcctg gtatagttcc
     1861 tatagtatta attaatccct caagagttaa agggactgga ataggataag atactctacc
     1921 agctatatct attaaatcac agttacaagc agaaaatctt tcaaaagtac catcaagtcc
     1981 agataaatta tcttttggag gagctgaaag atctatacct accaaattag cacctactat
     2041 aaagaaatct gagataaaag caaattgatt aaagtttaca aaaggtctta gagcatcata
     2101 tcttagaagt tctaaggcct ctctcatact ttttttacaa caacattgtt ttattttagg
     2161 ttctttatat ttatttggat aattatagta tctatcagct acttgataat tttcatagta
     2221 tctttcattt gtatttggat aatcttcctc atattcttgt gtaaatctaa agtcctctct
     2281 acatttatta ttttccacta aatacactcc ttttatattt taatttaaat gtatagataa
     2341 ttaagcttat ctatatagta atattatatt tataatgtaa aaaaatgtta caaaagatat
     2401 tataaacgaa taaaaaataa taatttgtca gataaacttg taaattttta tgaataatcc
     2461 attctaaaac aaaaaactgt gtataaaaaa ggtttatatg ttatatttat ataaatataa
     2521 catatgaacg agaaaggagt ggtttgataa atgattgagg tagataaatt gtgtaagtct
     2581 tttacaaggg ttgtaaaaga tgataagaat aaaagttcaa taagaaagct aaagaaagta
     2641 aaaactaaaa aagaagaatt ttttgctgta aataatgttt catttaaagt tatggaaggt
     2701 gaaattgttg gaatattagg tccaaatggt gcaggaaaga ctacattact tagaatgtta
     2761 ggtggcatat taacaccaac atcaggaagt ataaatattt ctggatatga ttattcaata
     2821 gatagaaatt cagcaaaaaa agaaataggt tatctctctg gaaatacaaa actatatgga
     2881 aggctttctc cgagagaact tctaacaact tttgcaagtc tatatgagat gtcaaaagaa
     2941 gatatagaag aatcaattga aaatattgta aaaataatgg atatgagtga atttatagat
     3001 aatagaattg aaaatctatc aactggtcag actcaaagaa catctatagc ccgttgttta
     3061 atacattctc caaaagtata tatttttgat gagccaacac taggtctgga tgttttaagt
     3121 agtgcatcta ttatagattt tatgaagaat gaaaaactaa gaggaaaaac tgttctttac
     3181 tcaactcatt atatggaaga agcagaaact ttatgtgaca agatttttat gattcacaat
     3241 gggaaaataa ttgcatcagg aacaccacag agtttaaaag aggaatctgg agtaaataat
     3301 ttgagagatg tattcataaa acttgccaga aaggaggaca tttaaatgag aagtaagatt
     3361 gtaaaataca tatttaaaaa agaaatgtta gacatattga gagataaaaa gactttattt
     3421 atgatgattg ttgtgccact tcttttatat cctttactta tgttattaat gattcaagta
     3481 atgaatatgt ctgcaagtag tatgaatagt aaggacatta atatagcttt taataataaa
     3541 cctaataagg tgttagtatc catgattgaa gatgataatt tagaaaaaga tactaaaaat
     3601 acttcaaatg ataaaggcaa aataagagtg ttagatgtag ataactataa aaagtcatta
     3661 gaagaaaata aaattgatgc ttatataaag atggaagaaa aaaaatcatc tataaattat
     3721 caaatatata taaattcttc tcaagaaaat tcttctaatg cactaaaaag aattgaaact
     3781 gttttggata aatataaaag atttaccata gaaaaaactt tatctgaaaa aggattagat
     3841 gtacaagcta ctttagaacc aataacatat agcactgtag atgttgcaaa gacggaagaa
     3901 gtggcaggat actttttagg tcagatactt ccatttatat taataatagg agttttacta
     3961 ggttcaatat atccagcaat agatgttatg gctggagaaa aggaaagagg tacattagaa
     4021 acattattta cactcccaat atcaaactta gaactggtta tgggaaaata tatggcagtt
     4081 tctcttagtg caatagtaac agcaatttta aacattttat ctatgggact gactatggca
     4141 tttattttag ttagtggagg tattagtagc caatttggct ttggaaactt taattatgga
     4201 gacttagtct taccaattat aactacttta atttgcatat gtcttttttc aatggtagtt
     4261 tcagcaataa gtatgtgtgt atgttcatta gcaaaaagtt ttaaagatgc tcaaaactac
     4321 ataacacctc ttatgttaat tattatgata ccatcatatg tttctatgat accaaatata
     4381 gtattggata gagtgacagc tgtaatacct gtagtaaata tatctttact tataaaaagt
     4441 gtattatctt ttaaaagcga tttaaattta atagccttag ttttagcatc aaatgtagcc
     4501 tttgttatat tggcaatcat actactgtct aagatgttca attctgagga aatacttttt
     4561 ggtaatagta agagtttttc tttcttagag aaaagaagta atataaaaaa agggtctata
     4621 ccaactgtaa gtgatggtgt tattttatat gcagtaggat tagttctatt gatttatgtt
     4681 ggttctttag ttcaaattaa atttggtatg gcaggtatta taatgacaca attcatgata
     4741 atttctttac cattattatt tgcatgctat attaaagctg attttaaaga ggtatttagc
     4801 ttgacagttc ctaaattaaa gcatgtgttt gggagcattg ttctttggat tggtggatac
     4861 atacttatta tgattataac acagctaatt ttattcttat ttcctcaaaa tatggaagtt
     4921 gctgaaagtt taaataaggc tctttttatc aaagatagtg ttttactgaa tttactagca
     4981 atagctttat taccagctat atgtgaagaa ctgtttttta ggggatttat attttcttca
     5041 tttagtaaat caaaagataa aaataagtct atcaaactcg ctataatatg tagtggggta
     5101 ctatttggta ttatgcatat ggattttatt agaataattc caacatcaat attaggtatt
     5161 atatttgcat acagtgtgta taaatctggt tcaatatttg tatcaatgtt attgcatttt
     5221 ttaaataata gtgtagcagt tgtgttaaat cattatacaa ctggaaatat aaccaactta
     5281 tataaatttg ttgaagttga tttttcgaac ttaaatgtac ttaaatttgc cttaattctt
     5341 ctaataagtc ttatattaat tatgttagga ttaagagttt tagatagaaa gtctttaaga
     5401 aattaagtat aaagttatta tatagattaa aatattaata agcactaaag tatatggata
     5461 tatcttgtat ataaaaatac tttagtgcta ttttatatat tttaattata tgaattattt
     5521 atcgtttcat ttatgtaatg aaaatgttat ataaatgaaa caataaataa ttttt
//
LOCUS       DAGQWR010000205         5558 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.208, whole genome shotgun sequence.
ACCESSION   DAGQWR010000205 DAGQWR010000000
VERSION     DAGQWR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.208"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            31..1170
                     /locus_tag="MIW95_003631"
     CDS             31..1170
                     /locus_tag="MIW95_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBY3152926.1"
                     /translation="MVIKLTIEEYKNKYSTGDKIILSLKSFDNKELYYHETDNILNSK
                     DEIDRLTSSKIFKIIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYNLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLDPLKIYNFMDELIIFMENSKVNDLVKSTIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLINKGYDTFKEFSISYIISKNRTKYYKAILDVENKGYNLTVFIEFMLKSIIQSINEM
                     RDMHDRKSLESILKEELFENDITLSATEEHILKYICSKDNYSMTLENYIKKNKGRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSREKDVYKVNEKYLNMLEIS"
     gene            1503..2078
                     /locus_tag="MIW95_003632"
     CDS             1503..2078
                     /locus_tag="MIW95_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152927.1"
                     /translation="MLKKKIISFLMAGIFVLSSGYNLVYASTFEENEYKQELVNENEF
                     TIINSNENEMIVDKKGNNTYVSTKVTPGSWKNVKYLGQKSRKLSSPYTRERIIQTIQW
                     GLGYLSGGSSFLLGLAESIYREKVYDITKYGGVYTKTYYRRQITDIRNSHANVPVPYR
                     YEYVTYFYSSSNFKKVVYTERVGDGIKLANN"
     gene            2089..2277
                     /locus_tag="MIW95_003633"
     CDS             2089..2277
                     /locus_tag="MIW95_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152928.1"
                     /translation="MNNKKKSFLISGTINVILLIIVFALFSYNIIENSTRNMIITVIL
                     IFIFEIIKIKILEKYYNL"
     gene            2472..3029
                     /locus_tag="MIW95_003634"
     CDS             2472..3029
                     /locus_tag="MIW95_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY3152929.1"
                     /translation="MVVGYMRVSTAEQNEDRQLVTMKKHNVEKMYQEKISAKDTKRSK
                     LNAMLDFVREGDTIIVHDFSRLARSTKDLLEIVELLEKKHVKLISDKENLDTSTPTGK
                     LMLTMLGAIYEFERTNMLERQREGISIAKEQGKYKGRKEVKIDNFGSYYDRYKTRELN
                     KTQLAKELGVSRPTLDRLIKSHESN"
     gene            3329..4072
                     /locus_tag="MIW95_003635"
     CDS             3329..4072
                     /locus_tag="MIW95_003635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152930.1"
                     /translation="MDNLERNIQLIFSYMAKTFIRNGATIREAEKEANEIIKTNSSNL
                     WGVNGLAYQLGKINLEFFCMYFIQDTYLPKEDNVAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGKKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKKFSDDVKYAA"
     gene            4268..4486
                     /locus_tag="MIW95_003636"
     CDS             4268..4486
                     /locus_tag="MIW95_003636"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152931.1"
                     /translation="MLFDFKNTNHLEDAKEFYWENEKEIKFPILWPSFWNCLDMALSY
                     YENPTSFKQEVQNDVNSIGEKWFKTIRT"
     gene            4526..5068
                     /locus_tag="MIW95_003637"
     CDS             4526..5068
                     /locus_tag="MIW95_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152932.1"
                     /translation="MLLCDPASAGGSKHDYSAFLVGSESENGLLCGRLAELAKINART
                     DFDKYILHMIYLLKVYPDITHVYIEKNTFNGTAANQLELKIKNDDVLYYRDIEIINEH
                     QKKNKDDKISTLIPVLNKGQMIFAEEDKAFIQQILNFTGQKYSLHDDAPDISAEFINR
                     IFNIKVNESITLLDRRNLGL"
     gene            5163..>5558
                     /locus_tag="MIW95_003638"
     CDS             5163..>5558
                     /locus_tag="MIW95_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBY3152933.1"
                     /translation="MYKYYKGETDAISNYKMVTKRSNNKINTNFLKKFINEEVAYSLA
                     NKITYTSRLGDEKIINDLEYYTCHWSKKHDSDLLRYTLLFGFCYELYYVKNNEMKVRI
                     IKPTDGCHYENEDGETIYFFREFKKDFKDD"
BASE COUNT         2349 a          549 c          892 g         1768 t
ORIGIN      
        1 gttaatatta atagataaag ataaactaag gtggtgatta aactgacaat agaagaatac
       61 aaaaataaat actctactgg agataaaatt atactttcct taaagagttt tgataataaa
      121 gaactatact atcatgaaac tgataacata ttaaattcaa aagatgaaat agatagatta
      181 acatcaagca agatatttaa aataatagaa gataaaaatg atttagcctt agagtcttat
      241 tattctagtg taatagaagg tgccttttca actagaaaga ttgcaaaatc tataattaga
      301 ggaaagatga aaccaagtaa taaaagtgag tatatgattt acaataatca tagagcctta
      361 gaatatggat tagataattt agataaatta tatagtcaca aatttatata taatttacat
      421 catatattgg gtgaaaactg tttagattca gaagaatatg aatatagaac tgaaaaagta
      481 tatgtatgtg attcaaaagg tgagattata catacaggac ttgatccttt gaaaatatat
      541 aattttatgg atgaattaat aatttttatg gaaaatagta aagtaaatga tttagtaaag
      601 agtacaataa ttcatttcta ctttgtatat gttcatcctt ttagtgatgg taatggaaga
      661 acatcaagag ccttatcata tctatattta attaataaag gatatgatac ttttaaagag
      721 ttttcaatat catatataat atctaaaaac agaactaagt attataaggc tatactagat
      781 gtagagaata aaggatataa tttaactgta tttatagagt tcatgctaaa gtctataata
      841 caatctatca atgaaatgcg tgatatgcat gataggaagt ctttagagag tatattaaaa
      901 gaggaattat ttgaaaatga tataactcta tcagcaacag aagaacatat attaaaatat
      961 atctgtagta aagataatta ttctatgaca ttagagaact atatcaaaaa gaataaaggt
     1021 agatacttaa aggctggaat aaaagaaatc gaattagttg accagttgat ggaagtgttt
     1081 aataatcttg aagaaataga gattttatct agagaaaagg atgtatataa agttaatgag
     1141 aagtacttga atatgcttga aataagttaa attgaagcat ataaaaataa aaagtatata
     1201 aagttatata atataataat aagccctcta tgaatcgctc ttaattgagt tttcttagag
     1261 ggttaaattt ttcattctta aaataaaaag atttttattt tgtccaaaat tacttttatt
     1321 attcgttata tatataaata atcttataag gaggtctaaa ttatatgata ttaaataaaa
     1381 ctattatgaa ataagacaag gtttgattgt gaagttatat aaaaagatta tttttaatat
     1441 aactaacagg ttatatgaca gtgtttatag aataaatatt taagtaaagg tagtaaaata
     1501 ttatgttaaa gaaaaaaata ataagttttt taatggcagg aatatttgtt ttatctagtg
     1561 gatataattt ggtttatgca tctacttttg aagaaaatga atataaacaa gaacttgtta
     1621 atgaaaacga atttactatt attaatagca atgaaaatga aatgattgtg gataaaaaag
     1681 gcaataacac ttatgtttca acaaaagtta cacctggtag ttggaaaaat gttaaatatc
     1741 taggtcaaaa atcacgcaaa ttaagttctc cttatacaag agaaagaatt atacaaacta
     1801 ttcaatgggg attaggttat ctttctggag ggtcatcttt tttattaggt ttagctgaga
     1861 gtatttatag agaaaaagta tatgacatta ctaaatatgg aggcgtatat acaaaaacat
     1921 actataggag acaaataact gatattagaa acagtcatgc taatgttcct gttccataca
     1981 gatatgaata tgttacatat ttttatagct catctaactt taaaaaagtt gtatatactg
     2041 aaagagtagg agatggtata aaactagcaa ataactagga ggaaatatat gaataataag
     2101 aaaaaatcat tcttgatttc tggcactata aacgtaattt tattaatcat tgtttttgca
     2161 ttgtttagtt ataatattat agaaaatagt acacgaaata tgataataac tgtaatttta
     2221 atttttattt ttgaaattat aaaaataaag atactagaga aatattataa tctataaaca
     2281 agaaatatct ttcctaagag catattgtaa agtatgctgg tacaaattta cattacaaac
     2341 tatctattct gatgtaaaaa taaaatcaaa actccaatta atttacaaaa agcatggcaa
     2401 atctttaact ctagttttac atttatgcta taatattatt tatagagtaa acataggtgg
     2461 aggtatcaaa gatggtagta ggttatatga gagtttcaac agcagaacaa aatgaagata
     2521 gacaattagt tacaatgaaa aagcataatg tagaaaaaat gtatcaggag aaaataagtg
     2581 ccaaagatac taaaagatca aaactaaacg caatgttaga ttttgtaaga gaaggagata
     2641 caataattgt acatgacttt agtagacttg ctagaagtac taaagactta ttagaaatag
     2701 ttgagctctt agaaaagaaa catgtaaagt taattagtga taaagaaaat ttagatacaa
     2761 gtacacctac aggaaaactt atgcttacaa tgcttggcgc aatatatgaa tttgagagaa
     2821 caaacatgtt agaaagacaa cgagaaggta tctctatagc taaagaacag ggtaaatata
     2881 aaggacgtaa agaagttaag atagacaact ttggtagtta ttatgataga tataaaacta
     2941 gagaacttaa taagactcaa ttggctaaag aattgggagt aagtagacca acattagata
     3001 gattaataaa gagccatgag agtaactaag aagagcaata tatatgctta agttaatttt
     3061 agtggggcaa attcatagaa ttatgccctt taattatgtt taaaataatt ataaataact
     3121 gtttttcagt atagggggta tccttctatt tcaaaaaagt attatttata gaaaatgagt
     3181 tctagaaatt ttctagcaaa aattaaaaac cagagacata tttcaaagag ggtgatttta
     3241 ttagaaaaaa atttgcattg tttattttat taattgtatt actcactgaa cctaaaaaaa
     3301 tagaagaatt aagaggaagt aaatacgagt ggataattta gaaagaaata tacaattaat
     3361 atttagttat atggcaaaga cctttataag aaatggagca actataagag aagctgaaaa
     3421 agaagcaaat gagattataa aaactaattc aagcaatctt tggggagtta atggcctagc
     3481 ttatcagctt ggaaaaataa atttagaatt tttctgtatg tattttatac aagacacata
     3541 tttaccaaaa gaagataatg ttgctgctcc tatagctaaa gttcatcatg agttatggga
     3601 agatatacaa gagtctataa ttggagatgg ttcacagcaa ttaggaagaa tttatccaag
     3661 aggaacaggt aaaagtgcat ttggagattt agcaacaact gtatggtcac attgttataa
     3721 acataagaca tatactttaa tttgctctga tataggctct acagcagaaa aattcgtaaa
     3781 agatataaaa aatgcattac ttgaaaatga gtatattaaa aaagcatttg gagttctctt
     3841 aaatgataat gatagaaaat atatttgcaa tagtactcaa ttagaattaa ctaataaaac
     3901 ctttattgaa gcaatatcat cttcatcacc aatgagagga aaaaaataca ataataatcg
     3961 tccagatctt atcatacttg atgattatca gtcagaagaa aatgttagaa cagaagatgc
     4021 tagagagaaa aaatttaaaa aattttctga tgatgtaaaa tatgctgctt aaaaaccagt
     4081 aataagaaat ggtaagatta taaaaagagg aacaactttt atagcattag gaaccttaca
     4141 acataaagaa tgtttctata gtaggctaaa gaatttacca acttggaaat ttaaatgtga
     4201 aaaaggagtt ttagttgata atgtagatga attatttaat tctggtttat ggcttaattt
     4261 caaagagttg ttatttgatt tcaaaaacac aaatcatctt gaagatgcta aagaatttta
     4321 ctgggaaaat gaaaaagaaa ttaaatttcc tatattatgg ccaagttttt ggaattgttt
     4381 agacatggct ttaagttatt acgaaaaccc aacatctttt aaacaagagg tacaaaatga
     4441 tgtaaattca ataggtgaga aatggtttaa aacaatcaga acttaatcta gagaatgtat
     4501 agaaactcat acattcaaaa agacaatgtt attatgtgac ccagcatctg caggtggttc
     4561 taaacatgac tatagtgctt ttcttgttgg aagtgaatca gaaaatggtt tactatgtgg
     4621 cagattggca gaactagcca aaataaatgc tagaactgat tttgataaat atatattgca
     4681 tatgatttat ttattaaaag tgtatccaga tataacacat gtttatatag aaaaaaatac
     4741 atttaatggt acagctgcta atcaattaga acttaaaata aaaaatgatg atgttcttta
     4801 ctacagagat atagaaatca ttaatgagca ccaaaagaaa aataaagatg ataaaatctc
     4861 tactttaata cctgttttaa ataaaggtca aatgattttt gcagaagagg ataaggcatt
     4921 tatacagcaa atccttaatt ttacaggaca aaagtactct ctacatgatg atgcaccaga
     4981 tatatcagca gaatttataa ataggatttt taatattaaa gtaaatgaaa gtattacact
     5041 attagataga agaaatttag gtctttgatt gtatggaggt gatgcattaa atggaaatag
     5101 atttaagttt attaagagaa atatatgaag actagagttt aaaaaagcaa gaatacaaca
     5161 ctatgtataa gtattataaa ggtgaaacag atgctattag taactataaa atggttacca
     5221 agaggtctaa taataagata aacactaatt tcttaaaaaa gtttattaat gaagaggtcg
     5281 cttattctct tgcaaataaa atcacctata caagtagatt aggtgatgag aagattataa
     5341 atgatttaga atactatact tgccattgga gtaaaaagca cgattcagat ttattgagat
     5401 atacactatt atttggattt tgctatgaac tttactatgt aaaaaacaat gaaatgaaag
     5461 taagaataat aaaaccaaca gatggctgcc attatgaaaa tgaagatgga gagaccatct
     5521 atttttttag agaatttaaa aaagatttta aagatgat
//
LOCUS       DAGQWR010000206         5548 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.209, whole genome shotgun sequence.
ACCESSION   DAGQWR010000206 DAGQWR010000000
VERSION     DAGQWR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.209"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            240..1067
                     /locus_tag="MIW95_003639"
     CDS             240..1067
                     /locus_tag="MIW95_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBY3152934.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2084..2548
                     /locus_tag="MIW95_003640"
     CDS             2084..2548
                     /locus_tag="MIW95_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3152935.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLS
                     KEDKRKNFISFINDNKELIGFINLLDEGSYVFFGIGIKPDYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYKSQGFKIVETQVQKTHLGDGEFFVMRYQ"
     gene            complement(2815..3459)
                     /locus_tag="MIW95_003641"
     CDS             complement(2815..3459)
                     /locus_tag="MIW95_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152936.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKSLSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPIKRKIR"
     gene            complement(3389..4678)
                     /locus_tag="MIW95_003642"
     CDS             complement(3389..4678)
                     /locus_tag="MIW95_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152937.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIDEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINLIWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFIMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4675..5547)
                     /locus_tag="MIW95_003643"
     CDS             complement(4675..5547)
                     /locus_tag="MIW95_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152938.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILDSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         2012 a          768 c          695 g         2073 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatat
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagttggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 aaatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggagaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggggttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 atggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     1141 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     1201 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca tttataaaca
     1261 tattaaggta atgatttctc ttggtaatta ttctcatatc ttattctttg attatattta
     1321 tcttattcct agcccttatg tcatattata tttcttatct tattatttat aattagcatt
     1381 aacttatcta aaatcttgta tttttttaat agtaaagttt acatctatat taaaaagttt
     1441 acatctatat taatttcata aaacaatcaa ggtagtcaag agattcaact ttatttgatt
     1501 ttaaaaattt aagtgaattt gttcttatac acatcttttt aaaattatat ctgtagaaaa
     1561 tatcatttag agctataatt aaattattaa attttagaaa atttattaaa aaatataagt
     1621 taaattaata tttttaaaga ttctttttta taactactag aatactaatt tagtagttat
     1681 tgtatctcta aagtttaagc atggatagat atacatttga taatttgcct ataatttacc
     1741 tactacttgg agtattggag caaatacaat atattaataa taagttccta aaaccaataa
     1801 attaactaat acacataaag atattgatgt tgattataca actataagaa acagatttaa
     1861 cagaatttaa tcaagctaag aaattacata tatcgtgatg tttttgaatt agaaggtaat
     1921 tcaaaattag tttatagtct tctgtgaatc caataaaaag tacagggtac aagaattgtt
     1981 agtaaaattt ttaaagattt tatggataga tataaatagt tttacttatt ttgtaaaatt
     2041 taaaatacca ttaattacct taaatatttt aataggagga catatgaatt taaataaagt
     2101 taaatttact gaagaacatg ctattgaagt tactaattgg aaatatgaag gtaaatattc
     2161 aatatacaac ctacctccat gggaggaaat taaaaagaag aatttttcac tatcaaaaga
     2221 agataaaaga aaaaatttta taagttttat aaatgacaat aaagaattga taggttttat
     2281 aaacttattg gatgaaggaa gttatgtatt ctttggtatt ggtataaaac cagattattg
     2341 tggaatggga attggcaaag aaataattag cctaggatta caagaatgta gaaataaata
     2401 tagtactaaa ccaattgtat taaatgtaag aacctggaac atgagagctg taaaatgtta
     2461 taagtctcaa ggatttaaaa tagttgagac tcaggtacag aaaacgcatt taggggatgg
     2521 agaatttttt gttatgaggt atcaatagtt tataacacta gtcatattgc taatatgaca
     2581 attaaaatga aaaaatacat gtgtgtatgt catagcaata agtgtctttg aaatataaat
     2641 ttgagagcct ttgataaaat tcggtataca tatatatttt atactacaga tatataaatc
     2701 tatctacata cataaagaga ttgtaagtag gtttgtgtat ataaaaataa atttatattt
     2761 cataaatgta ctttttattt gtattacctt tattttatct aagctctcat tttattacct
     2821 aatcttcctt tttattggtt ttattaagag agatgcaatt atcaacacaa cacttccaag
     2881 tatcagccaa tatttctcat caaaactacc ttgactcata gacattataa aaaattttcc
     2941 aacatacttg cctttagtca tcatatttaa aattaaatat cctatagata tcatgtaacc
     3001 tatgattgta ttattaaata aatttgagaa aaacattcct aaacttccta taaacatagc
     3061 tgatgcgaaa cttccaataa tatactcaac tactggaaaa ttacatccaa gatatattaa
     3121 aacatttaca aaacctatga ttaataccac tatacctata attgacatca gtatcctaat
     3181 taattgtaca ataacttgag ataatgattt tgaagatact atctcatcaa tttcttttga
     3241 ttgctctagc gaaaatacag gtaccaacat tactatccct attattgcaa cataattttc
     3301 taaacatata gctgttgaaa ttctgtctaa attagctact ccttgtatta gaggaatcat
     3361 acatagcaat ataattgaac atattaaatt agttaaagtg cttaatttga agttttcttt
     3421 tattagaact aaacagtttt gaaaactttg cccaaacatc aagattcccc cttctttttt
     3481 gctcatataa gaatatagct aacaaaacaa gcaccaatgc tcccaagctt atcacaattc
     3541 tattaatcat aatttgattg aagttttgta taataatatc cccatttaat acactattat
     3601 gtcttatcat tagccccatt gttgaataat cacctttgag tcctgtagaa tttatactgt
     3661 aaagccacca aattaattgc acaataattg ctactggtgt atctgttaat atagtaatac
     3721 tcattcctac agctgtaact atcataagag ttggtagtat ccaccataat gtatatttta
     3781 taaatgctaa gttatcaata gatatacctt tatctttagc aaagaaaatc aaaggaagta
     3841 aatctttcat acctaaaatt aatacaggca atatcattat aaagacaagt gcaaaatatc
     3901 ttgataaaac tattttgcat gaagatactt gttttgaata aataatctct ctcattcttg
     3961 aacgcttatc tttaagtgac ataaaaactg ccacaaatac tggaaatata cttaaaacta
     4021 tacctatata atcgcaaaaa attcttgcat acccattact aactttatct ttttgtattg
     4081 tatagttgta attcttcata gcttcatcat aggtaactgg aactactcca aatctttgta
     4141 acttgtcaaa agcatatgca gaccctcctc ctaaaatgtc gtctactctt tgcattattt
     4201 cttcaaatct ttcaagagat actttagaat aatctatttt tatactttca tcagttttcc
     4261 aaattaaatt tatttcgcta atacttttac cagttatttc ttctatatat ttttctatgg
     4321 tcttcgtttt attttggtct aattttatat acttaactac ccctgttgga tacgttgtat
     4381 atgtgttttt ttgatattct ctaattaggt ttatagttgc atttggaatg accttttctg
     4441 ttttcataga ttctttttga ccatatggat attgattgtg tacagagtaa tattcgtcaa
     4501 tattctctac tttttgtgga gcaactaatg gatttccatc attaggaata tttttactta
     4561 catcatttga aactgaactt ccaagttgag aaatatagaa aaaaactaaa actacaataa
     4621 aaatataata taccatactc tttaatatat ttttacattc ttttataaat agattcatat
     4681 attatctcct cccattataa gcatatagcc atcttcaata gttggattac aatttactgc
     4741 tgtctcaaaa ggtttatcat ctgatataat cctaagtata gcctttccac catgagtcaa
     4801 cattcctata actataaatc tactttttat attttcaatg tcttttttat ctacttcaat
     4861 actatatact ttcccttcag ctagttttgc taatgcttct gttgagccat tgtatatgac
     4921 ttttccacta tccaatatag ctacgttttc acatgtagct tctatatctc caacaatatg
     4981 tgtagataaa ataacgattc tatcatttgc aatttcgcat agtaaatttc taaatcttat
     5041 acgttcttct gggtcaagac ctgctgtcgg ctcatccact attaaaactc tgggattatg
     5101 aattaatgct tgtgctatcc caagccttct attcatacct ccagataaag attttacttt
     5161 cacattttga taattagtaa ggtttacttt gcttaataac tttgctataa tttttcttct
     5221 ttttcttagt ggtatatccg ataaaactgc taaataatcc attgctttat atactgtcat
     5281 gttaggatac attgaaaaat cttgtggcaa ataaccaacc atatttctaa tttccttagt
     5341 attctcaata ggaactccac acatatgtat tgaaccttct gtttttgata ataaagtggc
     5401 taaaattctc ataaatgtgg tttttcctgc tccatttctt cctaaaagac caaacatacc
     5461 actgtcgatt tcaagattta tattatcaag agcttgtttt ttcccaaatc ttttactaag
     5521 attggatact gctattttca tttccata
//
LOCUS       DAGQWR010000207         5493 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.210, whole genome shotgun sequence.
ACCESSION   DAGQWR010000207 DAGQWR010000000
VERSION     DAGQWR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.210"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            262..1098
                     /locus_tag="MIW95_003644"
     CDS             262..1098
                     /locus_tag="MIW95_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY3152939.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFKFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1451..3028
                     /locus_tag="MIW95_003645"
     CDS             1451..3028
                     /locus_tag="MIW95_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY3152940.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            3021..4205
                     /locus_tag="MIW95_003646"
     CDS             3021..4205
                     /locus_tag="MIW95_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY3152941.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     TL"
     gene            4223..4822
                     /locus_tag="MIW95_003647"
     CDS             4223..4822
                     /locus_tag="MIW95_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBY3152942.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4846..5316
                     /locus_tag="MIW95_003648"
     CDS             4846..5316
                     /locus_tag="MIW95_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY3152943.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIELKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         2074 a          570 c         1040 g         1809 t
ORIGIN      
        1 actgaaaata atttttgtaa tataaaatgt cgattgattt taaataatta aatatacata
       61 agaaataatt aaatatattt caatgaaaat atgataaaat atgacaaatt aattgatgac
      121 aataaaaaat attttgttat tgtagtatta tagggtaaat tgcaaaatat atacgaaagt
      181 aaaattttaa atattgacat taaattaaat atactatact atgtaagtgt gatgagagaa
      241 tttttttaga ggggatttat agtgaatata aaaaagatat ttaataacaa tgtagtagta
      301 agctccctag aagatggcac agaaataatt gtcactggtg ctggagtagg atttaagaaa
      361 aaagtaggcg atttgataga tgaaaatttg atttctaaga aatattttgt tcaagacgat
      421 caaagggaca aatacaatca aatacttaat aagacctcta ttgaatactt taaaatttca
      481 gaagaaatta tagaaaaggc taatgaagtt ttaaatactc aagtgaatga ttcaataata
      541 ttagccttaa ctagtcacat agaatttgct gtccaaagag aaaaacaagg tataaaatta
      601 ccaaacttaa tattgaatga gacaaaacaa ctttataggg aagaatttaa atttggatta
      661 tgggcaattg atgaaattga aaaaaggata ggaataaaat tgcctgaaga tgaagcagga
      721 tatattgcta ttcacataat caatggtttg gaaaattctc aaaaagatga aggtattaat
      781 atactagagt tttcaaaaca agttattcag ataattgaag aagtacatgg atttaaattg
      841 gatgtaaatt cattaaatta tactagactt ataactcact taaagttttt tgttcaaagg
      901 attctccgaa aagaaactta caatgatagt gatattgaag atatgtataa gttagtgaat
      961 aaaaattaca ataaacctaa agtgtgtact gaagaaattg cagcattggt attagacaga
     1021 tttgaatata agattagtaa agaagaagaa ttatatttaa tgattcacat aaataaaata
     1081 acaaacagtg gttgttagga ttgtaactga taaagcaggc aaaacctaag tcaatgagct
     1141 taactattag attaagtttt ttgatttagg tttttttatt agatacaatc aaaatacaga
     1201 cagtgacttt taggattgta actggttaag caggcaaaac ctaaatcatt gaatttaact
     1261 attaggttaa gttttttgat ttaggttttt ttattagata caatcaaata cagacagtga
     1321 cttttaggat tgtaactggt tgagcaggca aaacctaaat cattaaactt aattattaaa
     1381 ttaagttttt tgatttaggt ttttctataa atttgattat gtttttagat aaaaatagga
     1441 ggggaaaatt atgaaaaaaa ataagttagg tttatggaac ttcttccaaa tgttagggaa
     1501 aacgtttatg ttcccaatag cactacttag tgtttctggg atgatgctag gacttggggc
     1561 aggttttaca gaccctaaga tgattgaaat ggttccattt ctagggaatg aatatgttaa
     1621 cttagtttta aacttcatgt taacaatagg attatttgca tttaacaact tgggagcatt
     1681 atttgcaatg gcaattccac taggattatt aaaaaaagaa aaagagtttg gtgctttttc
     1741 aggattagta ggatttatag caatgcatat aggtacaaat ttttatttaa caagagctga
     1801 tttgttagta ccagcagaac agatgtcaac taatggtcaa gctatgatta tgggtataca
     1861 aacatataat acaagtgttt taggtggtat tgtagcagga ttaattgttt atgcaatata
     1921 tgataaagtt agtaatttaa agataccaga atcattagga ttctacagtg gaccaagatt
     1981 agttccaatt gttagtttaa tagttatgag tatagttgga ttattaatac ctattgtttg
     2041 gccaccattt ttcaatgcat tccaaagttt aggaagatgg atttcttcag caggaccatt
     2101 aggatatttt tcatatgcag tggcagaaag agttacaatt ccatttggac taaatcatct
     2161 tgtaacatct gtattccgtt ttacaccaat aggtggaact gctgttattg atggagaaac
     2221 gtactttggt acattgaaca tgtttatggc atatgtagaa aataatcaga ttatacctct
     2281 tgatttagct ggtaaaatgg aacaaggtaa attgatgatt caatatggat tagctggtgc
     2341 tgcattggct atctaccgta cagcaaaacc agaaaataga aaggcactta aaggactttt
     2401 aatatcagga gtgttgacag ttattatagg tggtataagt gaaccaatag agtttctatt
     2461 cttattcata tcaccagcat tatttgcttt ccatacattt atgaatggtc tagcaaacat
     2521 ggtacttcca tacttaggcg tgttgatggg attcacagga gatttaattg catttataag
     2581 ttttggtgtg ttaagaggaa cagctacagg atggccaata gcagtattag tagcagtttt
     2641 atattttgca atatactatt ttgtatttaa gtgggcaatt attaagttta atataaagac
     2701 cccaggacgt gaggataaag aaataaaagt atctgaagga aatggttcag atttcaaaaa
     2761 tttgtctaca tataaaggac aacaaatgat atctgcttta ggtggtcaat ctaatatatt
     2821 atcactagat aattgtgtaa ctagattgcg tttaaaacta caagatgtat cattaataaa
     2881 tgaggatgca attaaagagg ctggtggtat agcagttgtt aaattggatg agcatacaat
     2941 acaagtaatt ataggaactc aagtgtattc tttaagaaag cagatggata aagctatgga
     3001 aggtcatttg gaggcatgta atgattaatt ttgataaaaa agtagaccga ttgggtacat
     3061 attgtaccca gtgggactat gtagaagata gatttggtaa gaaggatttg cttcctttta
     3121 caatttcaga tatggattta gagtttccat ttgaaataac agaggtgttg caggaaaggt
     3181 taaatcatag agttttggga tacagtagat ggaaacatga tgattttaga aattcagtta
     3241 cgaattggta tattaaaaga tttaattgta gcgttgatag taactggata tattatagtc
     3301 caagtgtaat gtattcaatt agcaaactta ttgagatgtt aagtaatgaa ggagatggaa
     3361 tccttataaa tactcctgca tacaatgctt tttatgatgt gataacaaat aacaatcgta
     3421 atattataaa gtcacatctt gtaaatagga atggattcta ttcaattgat tttaatgatt
     3481 ttgagaaaaa gtgtaaaaaa tctaagatat ttattttatg taatcctcac aatccaactg
     3541 gtagagtatg gagtgagtta gaacttgtta agatgatagg aatttgtaag aataatgatg
     3601 tcaagataat atcagatgat atacacatgg atattgtata tgacaataga atgacaccta
     3661 tcttaaatgt tgcaaaggac tatttagatt ctgtattcat ttgtacatca gcgtctaaat
     3721 ctttcaatat accagcattg actgggtcat atgtgattat accaaatgaa ggtattagaa
     3781 ataaatttga aaatatcact agataccgtg attttgtaaa ttcgccagca attctagcaa
     3841 tattagctac aatggtctgt tatgatgact gcgaatattg gttagatgaa ctattaaaat
     3901 accttaaaga gaatcttaaa tatacgattg attatataaa agataattta gaaccattga
     3961 aattggtcat gccagaaggc tgttactttg cttggataga tttttccgaa cttaaaataa
     4021 gtggtgagaa attccaaaaa ttattgatgg atattggaga agttgctatt atgtcggggt
     4081 ctgtatatgg tgaagaaggt gagttttatt taaggttaaa tgtggcatgt tcaagagaaa
     4141 aacttgaaga tggtttaaat aggattaaaa aaactatgga ttatattgca aaaagaactt
     4201 tatagtagga agggagatag ttatgggata tttaaaaaca atggttgatg ttatgactac
     4261 tgaaatgaat gccataagat gtttaataga tgaagcagga atagaatatg aaagtatagt
     4321 tagtgaaatt gcaaattgtg aaggcaaagt aatttttatg ggtgtaggaa aatctgcaca
     4381 tataggtaag aaattggcag ctacatttgc tagtactggt acacctagct tttttgtaca
     4441 tgcaacagag gcagtgcatg gagatttagg aatgattgaa agtaaggata ttacaatttt
     4501 aatatcaaat agtggaaatt ctatggaagt ggtaaactgt ataaaatata ttaaagcaat
     4561 aggttctaag acaattgcat ttacttcaaa cagaaattca gtactggcaa aagagtgtga
     4621 ttatgcttta atatatcctg caaaggatga agcagaccat ttaaatctag caccaacaac
     4681 atcatctaca attactttgg ttttaggaga ttctattgca tgtgcattat ctaagagtag
     4741 taattttggt tcttcagatt tttataagta ccatccagga ggaagtttag gtgagaagtt
     4801 aaaaacagct aacaatggat aaaagcaaaa aggagagtag tttttatgtt caaaatgttt
     4861 aaaaaaaaat caataaaatc acccatatca ggaaaagtaa tagagttatc taaaatacaa
     4921 gatggtgtat tttctcaaaa aattatggga gagggtgtag ctatagattc tacaggagac
     4981 attgtatatg ctccaatcaa tggaaagata gtagtagtag cagagactaa acatgcattt
     5041 gtaatagaat tagaaaatgg gatggaactt cttattcatg ttggtttaga tactgtaaat
     5101 ttaaaaggcg aaggttttga ggcattggtg agtatgggag aacaagtgaa ggaaggaaca
     5161 ccaatgttga aaattaaccg tcctttgatt gaattaaagg gtatatcact tattactcca
     5221 gttacaataa ctaatcattc agagtacaat atgaatacat gtaatgtagg caatagcgtt
     5281 gaaggtggga aagatactgt aattgagttt aaataaagaa ttaaaataca aggttaatat
     5341 ttataaatac ctataaactt atgttttata agattgattg tatggattta ttgtataaaa
     5401 aagaactctt taattataaa ttaaggagtt tttttatttt gtaaatattg tttagattta
     5461 tatagaaaaa cacttagtaa taaatagaat aaa
//
LOCUS       DAGQWR010000208         5481 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.211, whole genome shotgun sequence.
ACCESSION   DAGQWR010000208 DAGQWR010000000
VERSION     DAGQWR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.211"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..750)
                     /locus_tag="MIW95_003649"
     CDS             complement(<1..750)
                     /locus_tag="MIW95_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBY3152944.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQIT"
     gene            complement(913..1647)
                     /locus_tag="MIW95_003650"
     CDS             complement(913..1647)
                     /locus_tag="MIW95_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152945.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(1616..2629)
                     /locus_tag="MIW95_003651"
     CDS             complement(1616..2629)
                     /locus_tag="MIW95_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY3152946.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(2651..3421)
                     /locus_tag="MIW95_003652"
     CDS             complement(2651..3421)
                     /locus_tag="MIW95_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152947.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(3396..3686)
                     /locus_tag="MIW95_003653"
     CDS             complement(3396..3686)
                     /locus_tag="MIW95_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBY3152948.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(3817..3916)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      4536..4625
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4678..4854
                     /locus_tag="MIW95_003654"
     CDS             4678..4854
                     /locus_tag="MIW95_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152949.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(4969..5325)
                     /locus_tag="MIW95_003655"
     CDS             complement(4969..5325)
                     /locus_tag="MIW95_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3152950.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKDVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
BASE COUNT         1837 a          901 c          611 g         2132 t
ORIGIN      
        1 tgttatttgt ttttgagtat tgtatatata attcagaaga attgataagc ttgactttat
       61 tagaccttta tcatcaaact ttagtttttg tagataaaca tctggaaaat attcttttaa
      121 tatatttata tccaaatagt tatcactaaa actttcattt atgtatatat tacttacagt
      181 agctatatct ctaagctttt ctataaagtc taaatcattt ataattattt cagttggatg
      241 gatttttgca atttcttcaa ttaccttatc ttcatttaaa catgttgcat tagtttctcc
      301 tgtacttata tcaacataag ttagacctat attagtacca tccttataca aagataataa
      361 ataattattc tttttatttt caagtaaatt tccatctaaa actgtaccag gagtgatgac
      421 tcttataacc tctcttctaa caattccttt tgctgtagat gggtcttcca tttgttcacc
      481 tattgctact ttatatccat tttcaactaa ttttgatata tatgaattgg ctgagtggaa
      541 aggaacacca cacataggag cacgttcttc taaaccacaa gcttttcctg tcaatgctat
      601 ttcaagagct tttgaagcta ctaatgcatc ttcaaaaaac atttcataaa aatctccaag
      661 tctaaaaaac agtatacagt ctttatatct attttttact tcaagatact gtttcatcat
      721 aggagttaat ttattcatat ctatttgcat tatcaaacct ccttcttttc taattaaata
      781 ttatatcata gtagatgatt attattaaca caatatataa aattttgaca tataactaaa
      841 aacaatccaa aatgtatact cacattttgg attgctaagt ttaatgctta ttaaataaaa
      901 atatatatat ttttacaata aagatattat ttctttttta agtttaatga tattttcaag
      961 gttatcttca tttaactttc tactatctat aacaaattct ttctttacta ttgatggttt
     1021 ttttgaaata acataaattc tgtttgatag cattatagct tcgtcaatat catgagttat
     1081 aagaagtatt gttgaattta cttttttctt aacatctaaa agccattttt gcatggatgt
     1141 tttagttaat gaatcaagtg ccccaaaagg ctcatctaaa agcattatgt cattagaatt
     1201 tataaaagtt ctcaagaagt tagctctctg tctcattcct ccagaaagtt ctgaaggata
     1261 tttatcttca tatccgctta atccaaacac ctcaaaataa ggttttacca ttgacctagc
     1321 tttactcttt ttttcttttt ttaaaattag tggtagtgac acattatcta ttatagtttt
     1381 atatggcaag agtaaatctt tttgatacat ataactatat ttaccattta tattcacttt
     1441 gccactatca taatctgtaa gtttagttat aatattaaaa atagtacttt ttccacatcc
     1501 acttggccct aatatagaaa caaactcacc ttcataaaca tcaatactta tatcttttaa
     1561 aacttcaata tctttataag tttttgatac attttctata gatactttta cttttttatt
     1621 gtggtaaaaa ttcatttgtg taagcatcac ttgccttcat atccttatct attaatttat
     1681 attcttttaa gaatgatgta tagttgttcc atacactatc tttcatttct ccccatcgtg
     1741 gtgcatcatc tttgtacttg cttgctaaat attcttgact ctttaaagct agtttttcat
     1801 ctacttcagg tgcatgtttt actaaaattt ttgctgattc ttctggattt tttattgcat
     1861 attcataacc ttttgttgta gcttttaaga attttttagc aagctctgga ttatctttaa
     1921 gcacagtttc attagaaatt ataagtggtg tataataatc caatctctca tctaaatctt
     1981 ttactggtat aaagtttaag tcatatccaa ttaaatcagc tttaactgca tcccaacctt
     2041 caaatatcca tgcaaaatct acagttttca tagctgcaaa gaaatcatct tgaccagtat
     2101 taactatctt taacttatta aaatcagctc catttttctt cataactgct ttaaaaacag
     2161 cttcttctga tggagagccc caaccaccat aagtttttcc ttcaaaatct ttagctgttg
     2221 ttatattctt ttcttttggt gatgcaaacc cagaagtatt atgttgtata acagttgcta
     2281 cagcttttat tggaagtgga tcttcactag tctttgcata agtaacttgt tcttgatagc
     2341 ttataccaaa gtcagctttt cctgttgcta ctaaagtttc tgctccactt tcaggtggtt
     2401 gtactatttc aacatccaaa ccttcttctt tatagtaacc cttatcaagt gctacaaata
     2461 atccagtatg atttgtattt ggtgtccaat caagcaccat agttactttt ttagaagaag
     2521 tatctttatc attgtttttg tctttattgt tatttgaaga acatccagtt attgttgcca
     2581 atgtaaaaac acttactaaa cctaatattc ctaacttttt aaaattcata attgcctccc
     2641 tcaaatacct ttaatcaatt aatctatgtc ttataacaac tttttttaca gcctgtacaa
     2701 ttcctacaaa cattaaacta aatataacta ctaatatagt agatgcaaat actttatcaa
     2761 gtgcatatgc actttttgac ctaagcatat aaacaccaag tcctttcgtt ccacctagcc
     2821 attctgcaac agttgctgcc attacagcat acgttgcaga tattttaagt ccagaaaaaa
     2881 atttatccat tgccatagga aatttaaggt gaatgaatgt attaacttta tttgagtcca
     2941 tagtcttaaa taaatttaaa taatctttat caatattctc aatgccatct acaaaactta
     3001 ttaatatagg aaaaaaacaa gtaagcatta ccatcaatac ttttggcatt gttccaaatc
     3061 caaaccatat cataaaaagt ggtgctataa ttattgtagg tatcatctgt gtaaccaaca
     3121 taataggata aagacatttt tttattattg atacaaaatc catcaatatt ccaattacca
     3181 atgatattat aattgcgact ataaaaccaa gtattgcttc tcctatagta accacagcat
     3241 gcatggataa attttgataa tctttaaaaa acacattaat tatatctact ggtgtgggta
     3301 gtatatactg aggaacttct aaaactccta ccatagattg ccaaagaata agtataaaca
     3361 aaaatgttgc tattggatat atactatctt tgactttatc tatgatttct aagtttttca
     3421 tctatagtca gacctccagc tttttcatct actctagcta ctgttatgac tctttcacat
     3481 ccaagattaa aagggatttt atgtaccttt tttaataatt caaaaacttc atcaaaatca
     3541 ccttcaacag tagtgctcat tgctcctatt tcacatttaa gaccactttt ttcaatttca
     3601 gcaatacaag catctactac tttatacatt tcttcttcac ctttgtaagg tcttagtggc
     3661 attactgcta tgtctgcgat taccatttaa atcactcctt taaaaataat taatacctac
     3721 taggtatatt ttgtttttta ttaaaaaatt ttattactaa aaagatatta tatttaaata
     3781 aaagaaaagt cttatccaaa ataggataag acttattttt atcttaactg gtctccctac
     3841 actggcatta tccaaacagg ttcaaagggt cgagataaaa tctcctctca gcacattagc
     3901 tccccgaaat atattatatc ttttctactt gattatacta cacttatcat attttaacaa
     3961 attttttatt tatattagat attttatccg ttcaaaaata tctaatatac attgtacatt
     4021 attacaccaa ataaattatc ttaaaatggt aatttatcaa catcttccaa aactagatta
     4081 actgaatttt ctagttttta ttttgctatt tcaagcatat ttggatgtat atcaattcca
     4141 tacaaatatt gttctaaatt tctgttttca ctaatctaat ttgcaatcgt tacagattac
     4201 agattagaaa tagcttttag actctactta tctttatcta caaaaaatcc ttcaataaat
     4261 tcagtaataa tattcagtaa tctaaaaata aacctctatc tggattttta agatacaagc
     4321 cttttaggaa gtatcttttg tgataatata aaaattactc gtcattatca tcaaaatcct
     4381 cagcaaccaa cattttagat aagaatcaaa ctctataata taattatttt atccaattta
     4441 tttttgagaa ataaaatatt tttttaattt ttaaattttt taatatgcac cttgaaaaaa
     4501 gaaacgtttt cctatattat ggtaatagta gaataaaact aaataggcaa aactagagaa
     4561 atttagtgac gcaaagctat agggactaag acttatgaaa tgagttatgt cagccagttg
     4621 ccaaagagat attttctttt tgtttttatg aatgtttttt agggggatat atgatatatg
     4681 tttatggata aaatggatag atgtacacat atactaactg catatatatg cagttcacgc
     4741 gattactgca attttataga tacacaatta aatgatttta tactagaata tggagagaat
     4801 gtggtagaat cttgtttgca tcaagtgatg gtattggtaa gcagatataa ttaaagaaat
     4861 tttattttct agaaaaccct ttttagtttt atctacgttt tataattcat taaagaacta
     4921 atcaaaataa gcttcattat atatttttca tgaagtaaac agttgagttt atctcatata
     4981 ctgtttaccc caattacata attctaataa tatagattcc aatgatttac cacgtttagt
     5041 agacaaatat tcaacttttg gaggaatagt cggataagaa attctcttaa taagtccatc
     5101 tctttcaaga tactttaatt cttgtgacag tatcttatga cttatatgaa caacatcttt
     5161 ttttaattga ctgtaacgta ttggctcttg ttttaaaata tgccataaaa taattgccct
     5221 ccatttactt cctatcacag tcatggtaaa accaattgaa caattaaaat ctttttgtct
     5281 ttcttcaata tttggaaatt cttcaaaatt attatctata tccatctcaa tcctctcttt
     5341 caaaatagta agtatctaac taaaaagtgc attcttgttt agcgtactaa ttcgtaatat
     5401 aatcttgatt aaaaaatagc tacgcacact cgtgacttta gtcatgagtt agtagctaaa
     5461 atagtcaaca tatagggaaa c
//
LOCUS       DAGQWR010000209         5455 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.212, whole genome shotgun sequence.
ACCESSION   DAGQWR010000209 DAGQWR010000000
VERSION     DAGQWR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.212"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..98
                     /gene="rrf"
                     /locus_tag="MIW95_003656"
     rRNA            <1..98
                     /gene="rrf"
                     /locus_tag="MIW95_003656"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(312..1496)
                     /locus_tag="MIW95_003657"
     CDS             complement(312..1496)
                     /locus_tag="MIW95_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY3152951.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1869..4220
                     /locus_tag="MIW95_003658"
     CDS             1869..4220
                     /locus_tag="MIW95_003658"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBY3152952.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERFEVQGNKKMKNFPFLMGQGVWKGSDDLVPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4242..4781
                     /gene="nrdG"
                     /locus_tag="MIW95_003659"
     CDS             4242..4781
                     /gene="nrdG"
                     /locus_tag="MIW95_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBY3152953.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2116 a          616 c          879 g         1844 t
ORIGIN      
        1 aggatacacc tgttcccatt ccgaacacag aagttaagct ctctagcgct gatggtactt
       61 ggtgggaaac tgcctgggag agtaggacgt agccacgtaa tttttttgtt ataataaatt
      121 tagaatttaa cttatatagt ttaatttgat aatttgatga aaaaaataaa agacttactg
      181 atttttaaat ctataggtct tttatttttt atctaacatt taaattaagt tgttgaatat
      241 atcttatatt acttacttta tatattaatt accattaata cattatatct aattactatt
      301 catctaaatt attatgccat tattttatat acaaattctt ttaatttgtc taatccagat
      361 aaaaaagtat tttcactgca agcataggat atacgaatat atttatccat accaaatgct
      421 atgccaggaa caactgcaac attatattct tctaaaaatt gattacaaaa ttctatagaa
      481 aaactatctt tatattcaaa tttctcagat actttagaaa gatctataaa tatatagaat
      541 gcgccatttg gattcacata tcctacatta tcaatagagt ctagttttga tttaattaaa
      601 tctcttcttg atttatatgt tttaaccata ttgtctatgt caattgagca atctttcaag
      661 gctccatagg ctatgtattg tgctgttaga cttgggtgag atataagatg tccttgtata
      721 gaactcattg ctttagcaat cgttttatta gatgcagtat atcctaatct taatccagtc
      781 attgcagctg acttagaaaa accatttata gttattgtta tatctttagc ttcttcgctt
      841 aatgatgcaa ttgatgtaaa ttcaccagtg tagcatattt tttcatatat ttcatctgct
      901 aaaatatata ttttattttg tatgcataca tctactattt ctattaattc atcttttgta
      961 taaacactac cagtaggatt tgaaggattg tttattacaa gaatttttgt tttatctgtt
     1021 atagattttt caagttcttc ttttgttaac ttaaatccat tttctttttt agtatcaata
     1081 aaaactggaa cagcattaac taacttaatc atttcaggat aactaaccca ataaggcttt
     1141 ggtaataaga cttcatctcc ttcgtctgtt aaagctagta aagtatttgt tattgagttt
     1201 tttgctccac ttgatacaac tatttcgtct attgaatagt tacagttatt ttcttcaata
     1261 agttttttac agatttcctc tctaagtatt tttaatcctg gaactaaatc atatttagtg
     1321 taatctttat ttaaagaatc aataccataa gattttgcgt tattaggtac attaaaatcg
     1381 ggttctccta tactaagatt tattacttta actccattac tttccatttc tttaacttta
     1441 gaactaatac ctattgtgta ggatggtgtt ataaagttta gtctttttga taacataaaa
     1501 tgcctccttt taaattatta tttatattat acgacaaatt taaagaattt acataaggaa
     1561 atttgtagat atatacatta atatatataa tagcctacac tttttgccta aaattagtgt
     1621 aaaaaaaaga gaaaaaaatt ttaactttca aagttgttag aaacgctact gttcaaaata
     1681 tccacatagt atttgcaatg atttgcgcaa gtttgactat tatgtacaaa tttggtaatc
     1741 ttaatatagc aattattaag ataatccctt taacttagtt gaaggggatt tttttagact
     1801 taacatacta catatagtgg tattgggatt aataaaatac aatatatagt aacaggaggt
     1861 ttttttaatt ggttgaattc gtaaaaaaaa gagatggaag agtaattcca tttaatgaag
     1921 atagaataac aagagctata tttttagcag caacgaatgt tgcagaaaga gaaggaatag
     1981 tacctgatta taaattatct gagcaattaa ctcaagaagt aataaagttt ttaaatcata
     2041 aatactcaga atcagttcca agtgttgaag atatacaaga ttctgtagtt aaggttctta
     2101 tagaaactgg ccatgcaaaa actagtgaag aatatataat atacagaact gaaagaagta
     2161 gaataagaaa ttcaaaaaca agattgatga aggcaataga ggaaattacc tttgaagatg
     2221 cagaagatgc tgatataaaa agagaaaatg caaatattaa tggtaataca gcaatgggaa
     2281 ctatgttaca atatggaagc actgtatcta aagaattttg taaaacacat atattaaaac
     2341 cagaacattc ttttgctcat gataatggag atatacacat acatgatatg gattttttaa
     2401 atatgggaac attaacttgt tgtcaaatag atgttaaaaa actattcaat ggtggatttt
     2461 caacaggaca tggcttttta agagagccac aagatataat aagttatggt gcattagctg
     2521 caattgctat acaaagcaat caaaacgatc aacatggagg tcaaagtata ccatttttcg
     2581 attatggatt ggctgaaggt gtatataaga catttaagaa attctacata ggaaatttag
     2641 ctaaagcatt gaagttattt aaaggaattg aaaataatga tgtgataaag aacatagtat
     2701 ataatacaga gaaagaaaca aatcaaaaag ttggtcttaa gagagatgaa ttgtatctaa
     2761 acttagaaaa agagaaatta atacagactt ttgatataga tgatgaactt gtaaataaga
     2821 tgcaaaactt tgcatttgaa gaatcgtata gagaaactga taaaaaaaca tatcaatcta
     2881 tggaagcatt tatacacaat ctaaatacaa tgcactctag agcaggtgca caggtaccat
     2941 tttcaagtgt aaacttcgga acagatactt ctgaagaagg aagaatggta actaagaatc
     3001 ttctattatc tcaagaaaga ggtttaggta atggagagac accaattttt ccaattttaa
     3061 tatttaaggt caaagaaggc ataaatttaa atcctgaaga ccctaattat gatttattta
     3121 aattatcatg cagagtatca gctaaaagac tatttcctaa ttttagtttt ttagatgcgc
     3181 cttttaatgc aaaatattat aaaaaaggtg aaccagatac agaggctaca tatatgggtt
     3241 gtagaacaag agtacttagt aatgtgtgtg gttcagaaac agttagtggt agaggaaaca
     3301 tatcttttac tacagtaaat ttaccaagat tgggtataaa acatggtatt ataaataatg
     3361 aaaaggctaa tttagatggt ttctttgaag aattagatga gaagataaac ttaattatag
     3421 aacaattatt ggagcgcttt gaggttcaag gaaataaaaa gatgaagaat tttccattct
     3481 taatggggca aggtgtatgg aaaggttctg atgatttagt accagaagat acactaaaag
     3541 aagtaataaa acaaggaaca ttgacaattg gatttatagg tttagcagag tgccttattg
     3601 ctcttatagg taaacatcat ggagaaagta aagaagctca agaattagga ttaaaaatag
     3661 tgtcacatat gagacataaa atggatgaag caacggacaa atataaatta aatttctcct
     3721 taatgggtac tccagcagaa ggtctttcag gtaggtttac taaaatagat aaaaaagttt
     3781 atggagaaat taaaggtatc acagataaag aatattatac taattcattc catgtaccag
     3841 tttattacaa tataagtgca tatgataaaa ttgagataga agctccatac catgaattga
     3901 ctaatgcagg tcatataaca tatgtagagt tagatggaga tccatctgat aatttagagg
     3961 cttttgaaac tgttataaaa gctatgaaag atttgggtat aggatatgga agtataaatc
     4021 atcctgtaga tagagaccca atatgcggtt tttcaggtgt aataacaagt aatatatgcc
     4081 ctgtatgtgg aagaaatgaa gatgaaagtg acatcaaatt cgaaagaata agaagaataa
     4141 caggatactt agttggtaca gttgatagat ttaataatgc taaaaaagca gaagtaagag
     4201 atagagtaaa acatagatag ttttatttgg gggaataagt tatgaaaata agaatgtcat
     4261 ctactataag ttatgacagc atagttgatg gaccaggatt aagaatggta atatggactc
     4321 aaggatgtat tcataattgt aaagagtgcc ataatcctca gacacatgat ttatgtggag
     4381 gattttatat ggatacagaa gaaattataa ataaagtcaa atcattgaaa ttacaaaaag
     4441 gcattacgct atcaggagga gaaccatttt tacaaccaga accattagaa gaaatagcaa
     4501 gagaagctaa aataaatggg ttagatgtat ggtcatatac tggatttacg tttgaacaac
     4561 tgttagataa aaaaaataga gcgtatttta aaaatttaaa tttactaaag caaattgata
     4621 ttttagtgga tggaaaattt atagctgaaa aaaaagatat aagcttaaag tttagagggt
     4681 catcaaatca aaggataatt gatgttcaaa aaagtttgaa atataaaaaa gtgtttttgg
     4741 ttgagcaata tatgaaggat gatttatcta tagcagaata aaatataaat aaacaggatt
     4801 aaatatatgc atatatttaa tcctgtttat tttcatataa tatttcaagt ttgtataatg
     4861 taacaattaa accaaaagac tcattgtata tttgaaatta agttttttat agtttatagg
     4921 tgttttatgt ggattacgaa tgatatagta tcgatacatt atttaatcga ttcttattcc
     4981 aagaaacaat taatttatag aaattcttaa ttgttacaat ataaatatct agataagtta
     5041 taagatactg ataaataatt tataaagttt attaatctaa tattgtgtgt gaatgttaag
     5101 tggtcaagta gtctaaaaaa tgaaaaagat ttaaaaaagt gttttaacta aacacataag
     5161 tgttaaattc atgtataagt ataggttaga tacataagaa ataattatgt aatatctatc
     5221 tttgtaaaaa aatattaaat tttgtaattt gtggataaat atgtttataa tgtggataat
     5281 atttaatgta ataagttaaa gcaatatgaa tcaactgttt ataatgtgga taatattata
     5341 aatgtatgaa taattgcagt atttaaaaac aaaaaaagtt aaaaaagttg ttgactctaa
     5401 aagaaaaagt tggtatagta atacttgtcc tcaagaaaag ggcaaacaaa taaag
//
LOCUS       DAGQWR010000210         5450 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.213, whole genome shotgun sequence.
ACCESSION   DAGQWR010000210 DAGQWR010000000
VERSION     DAGQWR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.213"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            53..847
                     /locus_tag="MIW95_003660"
     CDS             53..847
                     /locus_tag="MIW95_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBY3152954.1"
                     /translation="MVTIKEYSIPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            840..3146
                     /locus_tag="MIW95_003661"
     CDS             840..3146
                     /locus_tag="MIW95_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY3152955.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVAESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRIIRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            3285..4562
                     /locus_tag="MIW95_003662"
     CDS             3285..4562
                     /locus_tag="MIW95_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY3152956.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIESLKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            4745..>5450
                     /gene="yqeC"
                     /locus_tag="MIW95_003663"
     CDS             4745..>5450
                     /gene="yqeC"
                     /locus_tag="MIW95_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBY3152957.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQK"
BASE COUNT         2227 a          576 c         1031 g         1616 t
ORIGIN      
        1 aaagatttat taaaaacttt tgtgttttaa taagttaatg atgggggata agatggttac
       61 tataaaggaa tattcaattc cacagagttt agaagaagca tataaaattt taatttctaa
      121 aaaaaataat gttattttag gtggatgtgg atttataaaa ttaagtaata aaaatatagg
      181 aacagcgata gatttaaaag atataaatct aaattatata aaagaggatg aaaaaaatat
      241 tttgataggt gcagatactt cacttagaag tttagaaatt gataaaacta taaaaaatta
      301 ctgtagtgga atactttcaa gcgctgtttc taatatagtt ggtgttcaat ttagacaagg
      361 ggctagaatt ggtgcaagtg tattttcaaa gtatggcttt tctgacttaa taccagcgct
      421 tttggttgtt ggagcgaagg tgagattata caataaagga atactagagt taagtgagtt
      481 tttggatagt gaattaagta gggatatatt ggtcgaagta atactaccaa aaaaagatgc
      541 gatagctgtt tttgactcaa taagaaaatg cacaggagat ttttctgtat taaattcagc
      601 aatgctaaaa gaaaatgata cttacaaaat agcaattggg gcaagacctc aaaaagctag
      661 attagcactt gaagcgagta atatattaaa taaagaaaaa gatatagata aggcatctat
      721 tgttgctagt aaggaactta cttatggaag taatatgaga gctagtaaag agtacagaaa
      781 ggatatggca agtgcattag ttaaaaggat gtataatgct attgaggagg gaatgtataa
      841 tgattaataa ggggataaga aagatagata gtacagcaat tgtcacagga aaacctctat
      901 atactgaaga cctcattatg cataaagatg ttttgacatt aaaactactt agaagtccac
      961 atgctcatgc taagataaag aatatagata catcaaaagc cgaaaaaatt ccaggggttg
     1021 tgggtattta tacgtataaa gatgtaccac aggtcagata ttgtgtgcca ggtcaaacag
     1081 aaccagaagc atcaccttat gatagagttt tacttgaaga tacagttaga tttattggag
     1141 atgaagtagc tttagtggct gctgaagatg agaaaacagc acttaaagcc ttaaaaatga
     1201 taaaggtaga ttatgaggtt ttaaagcctg tgtttgatgc aagagaagca gaaggaaatg
     1261 aagttgttat acatccagaa aaagacatag tagagccata cccttttgga ttggatgcaa
     1321 aaaataatat agtatcaaaa gaattttttg aatttggaga tgttgaagct aagcttaaag
     1381 aaagtgattt tgtggctgaa tcttcataca aaacccaaag tcaatcgcat tgcatgatgg
     1441 aaactcatag gtcatattca tataaagata taaatgatag aattgttgta gtttcatcat
     1501 tacaatcacc acacaatgta aagagaataa ttagaaaagt attgggtata ccatcttcaa
     1561 aaataagagt aattaaacct agaataggtg gtggatttgg aggaaagaat atagcagcaa
     1621 cagatatatt tactgcattt gttactatga aaactggtag accagcaaaa tttgtattta
     1681 caagaagtga aacttttgta ggttctaata gtagacatga aatgtttttt gatgttaaag
     1741 ttggtgcaga taaagaagga aatttaaaag caatagagct aaagtcatta aacaatacag
     1801 gtgcttatgg tgggaatggt gtctctgttt cttccgaatc tggacacaat accttaccaa
     1861 tatataatga tattgatgct gttagattta atgctagaac agtatatact aataggttac
     1921 cagcaggagc acttagaggg tatggtgcta cacagggaat atttgcaata gatagtgcta
     1981 ttgatgaatt ggcacataag attggaatgg acccacttga atttagaatt aaaaatataa
     2041 tagataggta taccaatgga aaagcagctg aagacgaaat attaagttgt gagctgagac
     2101 aatgtataga aaagggcaag aaattaatca attgggatga taaatatcca tgtaaagata
     2161 taggaaacaa caaagtaaga gcagtaggta tggcagtagc aaatcatggt tctggtatac
     2221 cgcgtgtaga tatggcaaca gtcactataa gaatggatga agatggcaca tataaactat
     2281 tatctggtct tgctgactta ggtcaagggg ctgatacaat tcaagctcaa attgctgcgg
     2341 aagctttaaa tactactgta gatagaattt ctatttatac aggtgattca gatttatgtc
     2401 cctatgatac aggagcattt gcttcttgta caacaacggt tacaggaaat gcaacaataa
     2461 agacagcaaa taaattaaaa gatatattac tttcagttgc agagtcaaag ctaggattta
     2521 aaaaagaaga tttagagttg ggaaaagaca gggtttttgt taaaaacgat aatagcaagt
     2581 ttgttctatt agaggaattg ggtagagaat ctgttggagg aataggattt gctagtgatg
     2641 atgtaccagt agttttggaa gcaacagaat cttttggcat gttgaaaaaa ataaaaccat
     2701 ttatagcagg atttgcagag atagaattag ataaaaatac aggcgaatat aaggtcataa
     2761 attttgcatg tgtacctgat tgtggaacag ttataaatcc aactttagct agaatacaag
     2821 tagaaggtgg tgtgatgatg ggtatagggt ttgcaatgta tgaagaccct atatttgata
     2881 aagatgggag attattaaca gatagtttcc tacagtataa agttcctaca aaaaaagaag
     2941 ttggaaatat attggtagat tttgctgaga atttcgaccc aggaggtcct tatggtgcta
     3001 aatcattagg agaaatagtt atacatacac cagcgccagc tatagcaaat gcaatattta
     3061 atgctacagg agttcgttta agagagcttc ctatgacttt tgaaaaagtt tatatgggta
     3121 tgaaaaataa taataaacaa ttataataaa taataaggtg tataaaagca agtaaattta
     3181 aattattttg taaaataaag taattaaatg gacattatat atggtgtttt gtaaaaatac
     3241 cgtatataat gtctagtaga gtatattcta aaggaggtga gttcatgtca aacagaataa
     3301 aaaatataca ctatggaact cagagacaac aattaaaaaa cttaactaaa aaagaatatc
     3361 aagctctacg agagatgtgc ttcttagcta agaacttata taatgttggt acatataatg
     3421 taagacagca ttatttcaat caaggagaac atcttaacta tgaagctaat tatcatgtat
     3481 gcaaagaaaa tgagaactat aagcttctca atagtaatat agcgcaacaa atattaaaag
     3541 aagtagacag ttcgtttagg tcattttttg ggttaattaa attagccaaa gaagggaaat
     3601 atgactttag agggataaaa tcacctaaat atttagataa agaaggattt tttagcataa
     3661 taataggtca aataagaatc aaagaagatg ggattttaaa tgtacctatg tctccagcat
     3721 ttaaaaaact atatgggaaa gtaagtataa aagttccaca aaatatacta gagaaaaaaa
     3781 ttaaagaaat aagaataata cctaaacata aagctaggtt ctttgaaatt cagtattgtt
     3841 atgaaatacc gaaatcagag gaagtttcaa atcaaaaaaa tgcactagct atagacttgg
     3901 gtttagaaaa tttatgtaca tgtgttacca atttaggtga tagttttatc atagatggaa
     3961 aaaggctaaa atctgtgaat cagtgggcaa acaaacagaa tgataaaata gaatccttaa
     4021 aattaaaaaa gaatataact gcaataacaa gaaaacaatt taaagtttgg aataagagaa
     4081 ataatcaagt taaagactat gtaaataaaa cctgttttta tataattgat tattgtgtaa
     4141 aaaataatat tggaaattta attgtaggat ataatgctaa gttagaaaaa agtgataata
     4201 tggagagaaa aatatataga aattttataa gaatcccatt tggagagata aaaggaaaat
     4261 tagaatatct atgtaaaata aaaaatatca attttattag gcaagaagaa agctatacta
     4321 gtaagtctga tttttttgct gatgattttc tacctgatat caatgtggac aatgttaaaa
     4381 aatatgactt taaaggggaa agaataacac gtggacagta caaatccagt gttggaatca
     4441 ttataaatgc agatgtaaat gcgagtctta atatactaaa aaaatcacaa gtagttaatt
     4501 tatctaattt acagaataat cctcaaatat taaagcaacc acagagagta agaatatctt
     4561 aattatattt aaaagataaa ctgatagcgt aagcttacag cttagcggcg aattggatcc
     4621 gccttgttgt ttacaacaga atctatttac tttcgagttt atggaatttc aaaaatcact
     4681 tttactaaaa aggtctaact ataataatta agagattaga aaactggtta taagaggtga
     4741 aagtgtggaa ctatcaaata taatagaaaa gaatgaaata ataactgtag ttggagcagg
     4801 aggaaaaact tcgtttataa actattttgc aaatttttat agagataaat tgaaagttct
     4861 tcttacaaca acaactaaaa tatatgtacc aaacgattat gacaatataa ttattacaat
     4921 agatggaact gttattccat ctatttgtca tggaataaca gtctgtggca gttatataaa
     4981 taatgaaaat aaattagtaa gtattgattc ctctattttg gatgaaattg tagaccaatt
     5041 tgatttagtt ttaatagagg gagatggctc aaagagaaaa aaacttaaag gttggaatgc
     5101 taaagaacca gtagtatatc ataaaactac aaaaaccata gggatattag atataacatc
     5161 atttggaatg aatattaatg aagagaatat acatagagta gaaatattta aaaaaatagc
     5221 aaatttagat acaagtagta ttaatagtag ttctacagta agtattgaaa acttaaaaaa
     5281 tatagtatta aatccaaatg gattatttaa aaattattct ggaaaaagag tattatttat
     5341 taataaagtt gaaaatgaaa aatataaaaa tttagctata aaattaatag aaaatattaa
     5401 agaatatgaa agtgaaatag agatatttta tggaagcgta aaacaaaaat
//
LOCUS       DAGQWR010000211         5382 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.214, whole genome shotgun sequence.
ACCESSION   DAGQWR010000211 DAGQWR010000000
VERSION     DAGQWR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.214"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..777
                     /locus_tag="MIW95_003664"
     CDS             <1..777
                     /locus_tag="MIW95_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBY3152958.1"
                     /translation="KKSKRRKINKLKLTIVITVLFLALLSIYEILFSNPNKKGNIKSQ
                     ETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGKSMPTDEPISF
                     DSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEKIKLIDDYDAV
                     DEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDVTPKISTVYAD
                     RSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            803..2722
                     /locus_tag="MIW95_003665"
     CDS             803..2722
                     /locus_tag="MIW95_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBY3152959.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVKRGAQ
                     ARSTKQKARLQRFDELMNRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(2964..3311)
                     /locus_tag="MIW95_003666"
     CDS             complement(2964..3311)
                     /locus_tag="MIW95_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBY3152960.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(3298..5226)
                     /locus_tag="MIW95_003667"
     CDS             complement(3298..5226)
                     /locus_tag="MIW95_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBY3152961.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTTLPIVLSKVKNNEQE"
BASE COUNT         2064 a          824 c          767 g         1727 t
ORIGIN      
        1 aaaaagtcaa aacgaagaaa aattaataaa ttaaaattaa cgattgtaat aactgtatta
       61 tttcttgctt tattatctat atatgagata ctgttctcta atcccaacaa aaaaggtaat
      121 ataaagagtc aagaaactat gcagaataaa aataatacaa cagatgattc taaaaataat
      181 caaagctcta ccaaaaagca ggaagataat aatatcaagc ctactttttc atattcttca
      241 ataccagata atataaaaaa taaaatgatt ggtaaatcta tgcctactga tgaacctata
      301 agctttgata gtctatctta tttaaaactt acatattatg gatttgatga aaaaactcat
      361 caaggagaaa tgatagtaaa cagtgaacta gctccagaag tagtggatat ttttaaggaa
      421 ttatacgaaa aaaaatatcc aattgaaaaa attaaattaa tagatgacta tgatgctgta
      481 gatgaaaaat ctatgtctga taataatacc tcatcatttt gttatagaac tatagctggt
      541 acaaatgtag tttcaaatca tggtaaagga cgtgctatag atataaaccc attacagaat
      601 cctcaagttt ctggaaatga tgttacacct aaaataagta cagtttatgc tgataggtct
      661 agcacaaaat ttggaatgat aaaaaaaggt gatgattgtt ataatgcttt tgtaagcaga
      721 ggttggagtt ggggtggcta ttggaaaaac ccagattatc aacattttga aaaataatag
      781 attagaaata aaggtgaatt atatgaattt aatgacattg gaaaatataa gtaaaagcta
      841 ttctgaaaaa aaattactag aaaatatatc tcttggaata aatgaggggg aaaaaatagg
      901 acttattggt gtaaatggta ctggtaaatc taccttactc aaaattattg caggagttga
      961 agaaagtgag actggtaata taacaaaaac taatggtatt aggatagaat atctacctca
     1021 aaatccaact tacaatgaaa attcaactgt acttgaacaa gtatttaatg gaacttctga
     1081 agaattaaag ctacttggtg aatatcaaga aatcctagaa aaacttaatt caaactttac
     1141 agaggaatta aataaaaaat tattatcagt acacgaaaaa atagatgcct taaatctttg
     1201 ggatttagaa agtgaagcta agtcagtact tacaaagctt ggtataaatg actttaatca
     1261 aaaaatagca gaactttctg gtgggcaaag aaagagagtc tctctggcat cagcccttat
     1321 tacaccttgt gagtttttga tactagatga gcctacaaac catctagaca atgatactat
     1381 agattggctt gaggaatatc ttaattcaag aaaaggctca ttgcttatga taactcatga
     1441 tagatacttt ttagaccgtg ttacaaatag aattattgaa ctggacaagg gtagattatt
     1501 cagttatgat gggaattatt caatattttt ggaaaagaaa atggaaagac tttcgctaga
     1561 agcaagtatt gaagataaaa gacaaaattt aataagaaca gagcttgcgt gggtaaaacg
     1621 aggagctcag gctcgtagca ctaaacaaaa agctcgttta caaagatttg atgaacttat
     1681 gaatagagat tcttttaaac ctgatgaaaa tctcgatata tctgttgcat caagtagact
     1741 tggaaataaa ataatagaaa tacacaatat atctaaatca tttgaagaaa atactgttat
     1801 agataattta gaatacactt taactcgtac agatagaatt ggtataatag gcaaaaatgg
     1861 tatgggtaaa tctaccctta taaatatact taatggagaa atagagcctg atagtggata
     1921 tatttcaatt ggagaaacag ttaaaatagg ttgtttttct caagacgact ctcatatgaa
     1981 tattgatatg agggctatag actatgtaaa agaagctagt gactatatag aaacttcaga
     2041 tggtactagg attacagctt ctcaaatgtg tgaaagattc ctttttaatg gtactatgca
     2101 atatactttg ataggtaaat tatctggtgg tgaaagaaga agactacatc ttcttaaaat
     2161 ccttatgaca gctcctaatg tgcttctttt agatgaacct acaaatgatt tagatattga
     2221 aacgcttaaa atattagaag aatacctaga cgaatttaaa ggtgtagtag ttacagtatc
     2281 acatgataga tatttcttag atagaatttg taataaaata tttgcatatg aaggtaatgg
     2341 aaaaattcat atctatacag gtaattacag tgattatctt atttatagag agattcaagg
     2401 aattgaattt gaagaatcta aaagggaatc agttaaaaaa gatgactcta ctaccctaaa
     2461 aaaagaaaaa cctaaaaatg ataaaaagct taagttctca tataatgaac aaagggagtt
     2521 tgaaaatatt gactctgata tagaaaaact cgaatctaaa attgcaacac ttgatgaaag
     2581 tactgcaaaa ttttcaactg attttactaa acttcaagaa attttagatg agaaatctaa
     2641 actagaaaag gaacttgaat acaaatatga aagatgggaa tatttaaata atctagctga
     2701 tgaaatagct aataataagt gatattaaaa taatcttaag tttaataagt tttgctttaa
     2761 atctatactt tataaatatt ttaaaggtag tgtctcaaaa tgaacttttt agttttatga
     2821 gacactcttt tttatgagaa atcaaatatg ttttcatcat ttcaacaata caaaagaggc
     2881 ttatctctat tttgagacag cctctttcat aattaccttc taatttatat gctttaatat
     2941 atctataata attcttatta tatctatata tccagtcttt caatatagtg acaattaact
     3001 tcaatatgat ttaaaaatac taacaacctt tcataaagtg gatgagattc ttctccatat
     3061 ttagcttcta ggttcttgcc tatttctcta acagtccttt gaccatcaat ttgtaaaaaa
     3121 acaaaactgg aatattcatc tagttctatt tttttgtatt taggtattct agcttttaac
     3181 tttctaaaaa acttttgaat tttatggtcc tgtttttcaa gtatagtaac aacactattt
     3241 tcagctactt catattcaag tccatcagta attttaaata caatatctaa aacatcatta
     3301 ctcttgctca ttatttttta ctttagataa aacaataggt aatgttgtaa gcacaactaa
     3361 aactactaat aaagcaattg ccattgaatt tgttgcagca aatccacttg gtacctttgg
     3421 agttacaact ccagttactt gtaatattat acctattaaa ccaatgatag aaccaccagc
     3481 aacaagacca gaagataaac ttataccatt agaaacttta acttcttttt ctttttcagt
     3541 tttagatact aattcaacaa ataaacgaat taatgctcca actaatatta tagaagttgt
     3601 tgcaattggt aaatagaatc ctatagctat agtcattatt ggtaaattta atgcatatag
     3661 aactattgcc ataaatactc caacaataat cataacccaa ggtaaactac ctgacataat
     3721 accagaagtt aatgttgaca ttaagtttgc ttgtggtaat gcaaattgta cattatcacc
     3781 agttgctcta agttggtcag aaagtaataa aattactcct acaacaacta taacaccaac
     3841 tatacttgca attgtaaagt aacgttgcat ttcgtttttg ctaccaccaa taatataagt
     3901 tactttttga gattgagtat aacctccagc tatagcaata gcaacaacta taaatgaacc
     3961 aaataaaagt aatgatttat tagcttctaa atcagtccat cccattataa caaacactaa
     4021 tgttacaata actagagaag ctatagtcat accagaaact ggaagatttg atgttccaat
     4081 tgttcctgtt aaacgaccag ctactatgac aaataataaa gataataaaa gagatatgat
     4141 agctccaata attgccataa caatgttacc agaaattaag aatgctgcta aaaatccaat
     4201 aactactcca cctaataaaa gtatcatttg tatagatgaa ccttcttctc cttctccagc
     4261 acttgattta gctttaagtg tttcttttat agaagcgatt atagttggta taagttttat
     4321 tgcaccaata atacctccac aaagcatcat accagcacct atatatttta cataactact
     4381 agatattgct ccgacatcca tttggttaag aggcattgca ggattattcc ataccatagc
     4441 tccatcttta gccatatctg taaagtatcc tattaaaggt gctataccaa agtttgataa
     4501 aatacttcct gcaaacattg ttaaagaaac ttcaagccct actataaatc caattcctaa
     4561 aagtagagga tttacttcaa tatcaaattt ccatttgtag aatttgcttc ctacaagact
     4621 cattacattg tttgctacat ttaagaatga accagttaat acagtaataa atccacttat
     4681 tacaaatcca attcccatgt acttaattga atctccacca gcctctgaag caacaagtgt
     4741 ctcagatata gccattgatt ctggatacat aagtttacca tgttcttcaa ctattagata
     4801 gttgtgaact atagcagcaa ttcctatacc aaataaaact ccaccaactc caacagctag
     4861 gccttcaaag aatgtaattt ggcttccaat taatataact gctggtaata cgaaaataaa
     4921 tccacttgca acagattctc caccacttga catcccttgg attaagtttt taccaagaat
     4981 accctttgaa cgagcaaatg ctccaacaaa agcagaacct attatagctc ctggaatacc
     5041 tgcagcaact gtaagaccgg ctttcattcc agagtaagtt gtagatgcgg caaaaatggc
     5101 agctaaaata ataccgataa tcagtacagc tacatttcca ccggttctgg acttatctgt
     5161 gatatatgga acgtaatctt ttccactaac ctcaccataa gcacccttag gtaatttttt
     5221 gttcataaaa ttttcacccc ttttcaattt gtatatatta taacatatat acatataaaa
     5281 cttaactttt tattatttta caaatagcga gtcaaaacta ctaaaattta accctatatt
     5341 attaatttct acttttagat atttttatgc taattttctt tt
//
LOCUS       DAGQWR010000212         5127 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.215, whole genome shotgun sequence.
ACCESSION   DAGQWR010000212 DAGQWR010000000
VERSION     DAGQWR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.215"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            8..910
                     /locus_tag="MIW95_003668"
     CDS             8..910
                     /locus_tag="MIW95_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBY3152962.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIATF
                     GKLLIPFIMAGILGIVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            1519..2532
                     /locus_tag="MIW95_003669"
     CDS             1519..2532
                     /locus_tag="MIW95_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBY3152963.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKTFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFEIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            2888..3457
                     /locus_tag="MIW95_003670"
     CDS             2888..3457
                     /locus_tag="MIW95_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBY3152964.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKSIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(3794..4966)
                     /locus_tag="MIW95_003671"
     CDS             complement(3794..4966)
                     /locus_tag="MIW95_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBY3152965.1"
                     /translation="MIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPII
                     IGLNFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQY
                     LSNKIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAIL
                     ANIGVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIES
                     YLEIEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNID
                     IDNHLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKV
                     NPINRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
BASE COUNT         1878 a          511 c          847 g         1891 t
ORIGIN      
        1 tggaaatgtg ttgccacaag attattatgg attaatagtg attttaggtg ttattcttgg
       61 agttatgggg gcattttaca actggtttac tctatatgtg cagtctttat acaagaagat
      121 tcctaaaatt gctacctttg gaaaactgtt gattccattt ataatggctg gtattcttgg
      181 gattgttatg cctgatgttc tggggagtgg acacaattta gttgaagaac ttacaagtca
      241 tgaatatatt tttggaatgg ttgttttgat ttttgttata cgttttatat tttcggctgt
      301 tagttttggg tcaggtgcac cagggggaat attttttcca ttgcttgttt taggagcatt
      361 tataggtgga atatttggta tggtaggtgt taaagttttt ggaatgaatc ccgattatgt
      421 aaataacttt gtgcttttgg ctatggcagg atattttaca gctattgtgc gtgccccttt
      481 gactggtatc atattaattt ttgaaatgac aggttctgtt agtcaaatgc tttcactatc
      541 agtaatttca attgtggcat atattgtggc tacacttatg aagtctaaac ctatttatga
      601 aagtcttctt gagaggttac ttgaaaaaaa tggagaacct gtacataaag agaggggcga
      661 aaagatacta gaccagttct ctgttatgtt tggctctcct gttgcgaaca aggcaattca
      721 ggatgtacag tggcctgaaa attgtctttt ggtagcaatt caaagaggtg gagaagaaat
      781 tattcctaga ggtaagacaa ttcttagacc aagtgatgta attgtgacta tgacagatga
      841 gagagatagt ggtgctgtgt atgataagat ggaaaatttg tgtaaagaac atacgtcaca
      901 tattttttag ggataatgat gaatgtttaa taatgataga atatttaatg aagatttgat
      961 taaaaaattg gtaatttgaa agagagttaa ggaattaata gaaaatgaaa atgtgatatc
     1021 ttagatgtaa aagagtttga agaaaataat tgtatatatt tgttaatgag attatatgga
     1081 ctaatggtat atttatactg tgctaaaagt tgcactattg tatgaggaat gggaataaag
     1141 ggtttgaaaa aataggtaga agatttttca gatattctta ttgggatatt ttaattaata
     1201 tattttaatt gcgatatttt gggaagaata gaatttattt ataaatgaaa ataattaaaa
     1261 ggggttaagg ttgtattagc cccttttgct tatagagttt ggaaatggta tcttgatagc
     1321 agttgtttta tgcttattat attttattac atattttaaa attaacctta tcactcctaa
     1381 atcattattc tatcaacttt taaaacactt tcaagacaat cacctattga aaacaaagtt
     1441 aattaaaaac ctacatatca gtttagtatt cgtgtagatg attatataaa tataaaagta
     1501 taagaaaggt gagtatctgt ggttagttca tttttaaatg atatctttag aacaatttta
     1561 gtaagttgta tttttatagt tattttatta gtctttagaa taactttatt taagactttc
     1621 agtaaaaaat ttaattacta tatatggctt attgtgatta ttaagctatc attaccattt
     1681 atgaattata cttttatatt taatgagtca aaatataatg gtaatattaa taagataagt
     1741 ttaggcaatt ttaatagtat ttctattgta tatagtactg ttttagtctc tgtttggatt
     1801 gctattacga tattttattt agtttatact ttattgaaat atattaagct taaaaatatg
     1861 ataaatgatt tgtcttatga tgttgaggat gataagataa aaaatcttta tgaaaattta
     1921 ttaaaagagc ttaagataaa taaaaaaata cggcttaaat attcatatga agttgaaagt
     1981 ccagtatttt ttaattcgtg tgtcatttta ccaccatgtg attatacatt aaaagagtta
     2041 aactggatat ttaggcatga gttgatgcat tttaagagta aagaccttta cattaagtat
     2101 ttggtgatat ttttaaaatg tgtttattgg tttaatcctt ttgtgtatat aatggagaag
     2161 tttatagatt tggattgtga attttattgt gatgaaagag ttttgaagaa ttgtacgata
     2221 gaagagaagc aggattatgc attgacaata attagtgcaa tgagaaaagg ttcaaatagt
     2281 tcaaataaat ttgtggctgg attacataag gagtcagata ttaaaaaaag agtgtttagt
     2341 atgtttaatg aaaagtacag aaatggtatt ttgatagcag ttattttatg tttattatct
     2401 tctattacat attttgaaat tgatttaatt actactaaat caatatttta tccactttca
     2461 aaacaaacac ctattgaaaa tagaactaat tcaaaaccta catatcagat taatgtttat
     2521 atagataatt aaaaaataca aagtttaaca agagattaat ataaataatt ttaataagta
     2581 tctttcacat atgttaaatt tactttaatt atattaaagc caatgaatta ttctttagaa
     2641 gctccaatgc cagtaaatac aggtaaagaa aaatctttta ctacaattgt aaattaaaaa
     2701 atattctaat atttaaagaa atgcaaaagt gtaagaaggg taaaaatctg ttataataca
     2761 ttaaaaacta aatttagaaa aatttacata attagaaaaa tgatttgata aaaaataaag
     2821 ttaaatatta actaagtaaa tttgaaaaag aaaaaatata aagtacaaat ttaaaaggag
     2881 gtaactaatg aaaaagaagt tggaagatat gactttagag gagttatgga acttgtttcc
     2941 tatatttttg actactcaca aagaatgttg gaaaaattgg tttaaagaag aacaatatag
     3001 actaactgga atattgccaa tgcaaaaaat tgttagaata agtcatattg gaagtacttc
     3061 tgtgagtacg atttatgcca agtccattgt tgacatactg attgagataa ttcctgatta
     3121 taatatgaaa gatattaaag atttgattga gtcgaatgga tatatttgta tgtctgagag
     3181 cagtagtagg ctttcactta ataaagggta tacagtagat gggtttgaag aacgcgtttt
     3241 tcatttgcat ataaggtatg taggtgataa tgatgagttg ttttttagag attacttaat
     3301 tgaccaccca gatgttgcaa aggagtatga aaaacttaag ttggaccttt ggaagaagta
     3361 tgagcataat cgtgatgctt atacagatag taaactagat tttgttaggg agtttactga
     3421 aaaggcaaag gtgttgtatt ctaatagata taattagaaa taggtacaat tagatattta
     3481 tcttatatat cgtattatat tttttaagaa agtaattaat agaaaaaggg gttgtttcaa
     3541 aatggagaga taaatccctt tttcattgtt gaaatgataa aaatatattt gatttcatgt
     3601 aaaaaagagt gcctcatcaa ctaaaaagtt tattttgaga cacacacatt tttattaagg
     3661 taaataaaaa tatcacattt gattaaattt taagttaatg aatatatgtg taaatattta
     3721 attataagtt attataattt tacatagtat aaaacaatct aatttgtatt atataagata
     3781 atctaatttt agactaaaat aatctaatat aaagaaatag agaaaattca ttctcagtat
     3841 aagagatatc caattttcca ttgtacttat taacaatttc tctaatattc tcaataccat
     3901 atccgtgaga attactatct tttttatctg aaataaatag ttcttgtttt ctattgatag
     3961 gatttacctt gttgtttgtc aataaatagc taaaatactc cttctcacaa cgtgccttta
     4021 ataccacttt tctatttgac aaatcaggaa ttttcaaaga tgcttctata gcgttatcta
     4081 aagaatttga aaaaatagta cataaatcta taggttctat acccaagtga ttgtctatat
     4141 ctatattaaa gtgcacatca acaccatttt cttccaaact tgtatgacga agatttaaaa
     4201 ttgagttaag agcttggttt ttgcaaaatt gtttgccact aagagtacct atacctattt
     4261 ttaactgatt aaaatacttt ttagctccag caatatcttc aatttctaaa tacgattcaa
     4321 tcatttgaaa gtgattattc atatcatgtc gcattttgcg tatttgaatt tgttgatttt
     4381 ctagtaagtt atagtatttt tcttttaggt gatttgcttg tttttctatc ttcatcttta
     4441 cattttcaat caatggctgt aaaagaagta tcacaccgat atttgccaga attgctgaca
     4501 agatgattag ataagtgcca taaggtaagt tgcctaagat aatattctta ttactaccca
     4561 tagggattaa attagtaggt ggcagaataa tagcaattaa tattgataaa aatgaagata
     4621 tacataccat atcaataaaa atccatattt tatttgataa gtattgtttt atattgacca
     4681 atctattctt taggaaaata tgtattaaaa accaaaataa taatttaatt agagcagaaa
     4741 ttatctggac tattgaaagt attaaaatat attcttttga gtgtataagt tcctcatata
     4801 tagggttgtt tataaaaaga aaattaagcc caattataat aggataaagt atcatgacaa
     4861 ctgatattat aggaattaat ttgcttttgc tacatattat aagtagtata aaatatccga
     4921 atagtgtaaa caaaatattt acggggtcgg aaagccatat tatcatttga ctaataatag
     4981 agtagcccaa aatcaatatg attttgaaaa tccaatgatt cctaaagtct ataaaatagg
     5041 aagtcaactt ataaagaaaa aaggctatga aaatattgat taatattaga ataaaattta
     5101 tgcttgtaga gattaaagaa taagtgt
//
LOCUS       DAGQWR010000213         5094 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.216, whole genome shotgun sequence.
ACCESSION   DAGQWR010000213 DAGQWR010000000
VERSION     DAGQWR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.216"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..1767
                     /locus_tag="MIW95_003672"
     CDS             <1..1767
                     /locus_tag="MIW95_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152966.1"
                     /translation="NGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSISLL
                     VSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIETM
                     MLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVVLS
                     QFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYKEI
                     MVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQGI
                     LKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSIEH
                     IKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTLLK
                     ILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVYKS
                     NLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDADM
                     YIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKLLE
                     SGRHDELMDKKGVYFNLFCNCK"
     gene            1789..3210
                     /gene="ccpM"
                     /locus_tag="MIW95_003673"
     CDS             1789..3210
                     /gene="ccpM"
                     /locus_tag="MIW95_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HBY3152967.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVNKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            3239..4309
                     /locus_tag="MIW95_003674"
     CDS             3239..4309
                     /locus_tag="MIW95_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBY3152968.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            4325..4576
                     /locus_tag="MIW95_003675"
     CDS             4325..4576
                     /locus_tag="MIW95_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HBY3152969.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            4627..4830
                     /locus_tag="MIW95_003676"
     CDS             4627..4830
                     /locus_tag="MIW95_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152970.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
BASE COUNT         2074 a          450 c          817 g         1753 t
ORIGIN      
        1 aatggtaaat ttcaattttt taaaggtgta agaaattgtt ttaatatgat ttatctacat
       61 gataaaagat atttatttat ctctatgttt atggcaattt ttcaggggat tgttccatct
      121 atttctttgt tagtaagtca aaatatcatt aataatatgc aaacattttc gaaaagtgta
      181 aaagaaatat tagttttgtt aattatgtat ttaatcataa atataattcc ttcaattttt
      241 caaagttttt atggatatta tagaataaag tttaataaaa attttgaaaa acatattgaa
      301 acaatgatgt tagagaaatc tttaactttg agtttaaaag attttgagga tgataatact
      361 tatgacatga taaatagagc tcaatctcaa aaaggattta atattactat actatctgaa
      421 aaccttattt caataataag agaatcagta tctacacttt ctcttattgt tgtattatca
      481 caatttcaac tttggctaat aatagttgtt attgttattc ctgtattgaa atcattatat
      541 tccattaaat taggaaagga acaatatgat gttagtgtaa acagaacttg gaaagaaaga
      601 aaggcctggt acatagatta tttgttgtca aaaggttatg cttacaaaga gattatggta
      661 tttagaattg gttctaaact tattaatcat tataaaaaat taaaagagga tattatatct
      721 caagatatga atatagctaa aaagaatttt attgctacgc tattatgtac agttttagat
      781 tcaattgtat ctgcaggaat tataggattt ataataattc aaggtatttt aaagaagatt
      841 ttaataggag atgcgactac atatatacaa gcagttcaat ctataaagga taatgttgaa
      901 tcaatatttt cgttatttag ttcagtaata caagatcttt tttatacaga tttactgttt
      961 aaatttctag attataactg taaagaagac tcaattgaac atataaaaat atttgaaaaa
     1021 ataggggata taaataaaat agagttaata aatatttcat ataaatacaa tcattcatca
     1081 gagtattctt taaaaaatat taatttaact ttaaataaaa atgatcctat tgctatacta
     1141 ggaaaaaatg gttcaggtaa aacgacactt ttaaaaatat tattaggttt ttatgatgat
     1201 tatgaaggtg acatcttgat aaatggaaaa aatttaaagg atattgaaaa agatgaatac
     1261 attagaaaag taagttgtat gtttcaagat tatattaaat atgaggctac attaaaagaa
     1321 aatgtcgcca tgggagatgt atataagagt aacttggaag aaaaagttaa aagtaaactt
     1381 aaagaaacta aattgaaacc aagtatttat gaggaagatg gattagaaac tattttagga
     1441 acctggtttg gtaagaagca actttctatg ggtgaatggc aaaaagtagc aatatctaga
     1501 actcttatga aagatgctga tatgtacatt tttgatgaac cagattcatc tttagattct
     1561 gaagcgggaa atgaactcct ttctttatat aaaaaaacat ttgaaaataa gattggaata
     1621 tttataacgc accatatcca acatgcaaaa aagataacta acaatattgt agttcttgaa
     1681 aatggaaagt tgttagaaag tggaagacat gatgaactta tggacaaaaa gggtgtttat
     1741 tttaatttat tttgtaactg caaataaata ttaaagggag gttaattaat gaatcaaaaa
     1801 ccatttatac acacatttaa agctggaaaa tcatatttta tttatgatgt taatacggat
     1861 aagattttaa aggtaaatgc tgctgtttat aattatctaa ataaaattga gcataattta
     1921 gaaaaagaaa gtgactggaa catagaaata gaagacgaaa ttaatacctt gattaattat
     1981 ggatttctaa aaaataaaag ggtaaataaa acaatgcacc ctgaaactaa gtatataaaa
     2041 tatcatatag aaaataatct atcctctatt attttacaag taacccaaaa ttgtaattta
     2101 cgttgtgact attgcattta ttctggttca tacagcaaca gagttcatac aaataaaaga
     2161 atgaatatag atgtagcaaa aagaagtatt gactatttga ttaagaattc tagtcaatta
     2221 aatgaagtta gtgttggctt ttatggagga gaacccttac ttgagtttga tttgattaag
     2281 cagatagtag aatatgtaaa agtagttgga gaaggaaagg aaatatactt taatctaact
     2341 acaaatgcaa ctttatttaa tgaggaaata atagaattct ttgtaaaaaa taatataaca
     2401 actatgatta gtttagatgg aggaaaagaa attcatgata aaagtagaaa atttgcaaat
     2461 acaggagaag gatctcatga tgttgtatta aaaaatgtac gttacataaa aaataaatat
     2521 ccagaatatt ataaaaagaa tattacattt aatacagtga taaattcaga aaatgattac
     2581 atttctatag atgattttat atctaaaaat gagactttaa aagattcaaa ctttttagct
     2641 tccactctta ataataacta ttctgataaa gaaatagttt catcagatga atatatatta
     2701 ggtaagagtt atgatagatt cttgacttta ctcaacaaat taaaatttat aaaagatgag
     2761 agtatgtcac ctttatcaag aatagaaaga gccttattag atgattttag atatgagaaa
     2821 aatattgaag gtagattaga gttaccagat gtgtggcatc atggtggacc atgtatacca
     2881 ggggctttta gaatatttgt aactacagat ggagaatttt ttccatgtga gagagtaagt
     2941 gaaagttcta aaatgggtat gattggaaat ttagatagtg gagtagatat agaaaaggct
     3001 gaaaaacttt taaatatagg aaaattatat gaagagcaat gttctgattg ttgggcctat
     3061 agttattgtg atatatgtat agctaagatt gatgatacta aatgtaatga aaatgaaata
     3121 aaaccacatg attgtgacag aattagacat tctacagaaa ataatatgaa agattactgc
     3181 gttttgaaat ctctaggata tcaatattaa attttctaat gaaaggaaag atgggattat
     3241 ggaaaaagta cttatatatc catttaatta taattttttt actattttaa atcattgcaa
     3301 taaaatttca gatatagaaa ttaattcact ggttagtcct tctggctggg ggttatgcaa
     3361 taaggagtat aatgttaata atattattac taaagttaca aatgattttc aaagtgaact
     3421 taaaaagaat tctttggtat ggtttgttga ttcaaaagaa aatctagact tagaaaaatt
     3481 tataattcct aaattaaaag tagctctaaa agaaggtaaa aagatattat atacacgtga
     3541 tgatcttaat aaaatatcta ggtatatatc tcattatgaa ttcaaaaaga ttaatgaatg
     3601 gaatttagaa aaatatgaaa taagagatat agagggtata tcacagataa acacaccaat
     3661 aatatttatt tgtggtcttt atggagggtt agatactttt gatgtaaata taaggatagg
     3721 agaagaacta agaaaaaggg ggttttcaat attacaattt ggtcctaaaa aagagagcta
     3781 tttatttaaa atgattccta taccagagtt tatgtttgat gattcgataa gtagtaagac
     3841 aaagattatt aattttaacc attttataaa agataaagag gtagaggcaa agccagattt
     3901 aataattata gaagttccag gagaattatt ccctttctct agaaaaatgg ttggtaattt
     3961 tggaattaga gcttttgaaa tttctaatgc agtaataaat gattgtggta tattatgtat
     4021 tccatcaagt atatatgata gagaatattg tgaagaactt aaatatttta ttaaaggaag
     4081 acatggtata aatatagatt attttagtat agagaaaaaa gttatagata catatgagac
     4141 agaggtgaag caaaaatttt catatatgtc attagatgaa aacttttttg ttgaagatat
     4201 agaagattat gaaaaaacat tttttataca tgataataaa caattactag aattagtgga
     4261 caatatagtg aatcaattaa aagagtatgc taaagtaata tctatttaga attggaggtt
     4321 aattatgaat tacactgaaa aggaattact actagagaat atattttttg atgtgactgg
     4381 aattaatttt aataaaaata acaatttaaa aaaagaatta tttttttcac cgtctttaca
     4441 tatacagcca agagaattac ttttagtatt ttgcgaggta atagaaaact ttaatataaa
     4501 aattccagag gaaaaacttt tgaataggca atttacgagt tttgataatg ttctaaaaat
     4561 aatcaatatg gtataggtta ataaaggtat aaagtaagtg atcttataga aaaggggagg
     4621 atatgaatga gttttggaaa aagaatagaa atgaataata taggtgttga gacaagaggt
     4681 gtttgtacta ttatgtgtcc ttatacttgt aagatgatgg catcttcatc ttgtaactgc
     4741 aagaataatg attataatgc aagaggagga tcatataata catattataa taaattgata
     4801 catggtcaaa gaactcctga tggaatgtaa atatttcagt aataagtata ctattaaatt
     4861 gatttagcat atttgattaa atttatcttt cttactttat ttccttttat tataaaaata
     4921 tgcagttgat tataattaag agtgtgattt tataataact gcataaatag tatctgttat
     4981 ataaatcacc tcttcaatta ttatagtggt aaatatagtg attaggggtg atttttacaa
     5041 tttaaaagta taaattaatt aaaagttatt ataataaatt atacttattt aaat
//
LOCUS       DAGQWR010000214         5074 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.217, whole genome shotgun sequence.
ACCESSION   DAGQWR010000214 DAGQWR010000000
VERSION     DAGQWR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.217"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..202
                     /locus_tag="MIW95_003677"
     CDS             <1..202
                     /locus_tag="MIW95_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBY3152971.1"
                     /translation="YVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKIIE
                     SNRVDLEKKNIIRSMKRKTRRC"
     gene            216..890
                     /locus_tag="MIW95_003678"
     CDS             216..890
                     /locus_tag="MIW95_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBY3152972.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKRKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            916..1752
                     /locus_tag="MIW95_003679"
     CDS             916..1752
                     /locus_tag="MIW95_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3152973.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            1768..2439
                     /locus_tag="MIW95_003680"
     CDS             1768..2439
                     /locus_tag="MIW95_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3152974.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            2429..3661
                     /locus_tag="MIW95_003681"
     CDS             2429..3661
                     /locus_tag="MIW95_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3152975.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            3811..4128
                     /gene="trxA"
                     /locus_tag="MIW95_003682"
     CDS             3811..4128
                     /gene="trxA"
                     /locus_tag="MIW95_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBY3152976.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            4145..5023
                     /locus_tag="MIW95_003683"
     CDS             4145..5023
                     /locus_tag="MIW95_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBY3152977.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
BASE COUNT         2179 a          454 c          891 g         1550 t
ORIGIN      
        1 ttatgttaaa atgggtatgg catggttgct atcaatttgt tttattaaat gtgaagagga
       61 aacttttctc tatataaaaa ataataattt agatgatttc acttataata aaatgctaca
      121 aaaaataatt gaatcaaata gagtagattt agaaaagaag aatataataa gaagtatgaa
      181 aagaaagaca agaagatgtt gacggaggaa tattaatgaa taacaatgaa gacagaattg
      241 acaaattttt agaacttcaa agcaaaggat ttaaatttgc agagatagct caaaatctaa
      301 agattagtca aagtacttta agaacttttt taaataaaaa aggatataag tcagaaaaag
      361 gtaaatacat attaaaagac gaattaaatt ataataaaag tttggataat agaaacttgg
      421 aagaagagaa agctaaggag tatgaaaaaa gtaaaaagaa tactataagt aatagaaaaa
      481 aagtaagcac caaaaaaaag atttctaatg aaaaggcttt aagtaaaagt gttgaacaaa
      541 ttgagtttaa tgatacaggt ttaaaaaatg caaagcgaaa acaaaatgtt aagcctaaga
      601 aagatagaaa aataaatata actcaagaag atttagataa gctttgtgaa gtatatgatt
      661 ggtatttaga agtaaaagat tacaatataa agcctaaaaa agtatctaat aaaaaagatg
      721 tacatataga tgatgaacac atagatgaat taaaaacaac aagaatacgt atagataaag
      781 aaatatggaa tgattttgaa agattatgta gtaattcgaa ttttgaaaaa catcaaatac
      841 ttacacaggc attgaaagaa tttatgaaaa agtataaaaa tctactataa aatactacta
      901 tatgaggtgc ttaatatgaa taaaatagta aaaaaatcaa tttctctaat gattatatta
      961 acaatattta tttttatgtt gacagcatgt gaaaaagatg aacagcctga ttcagttgat
     1021 attcaaacag aagataaaaa tgagataaaa atagatgagg gaaatgcaaa agtattgaat
     1081 ataggaaaat cagaaattat aaatatagat gaaggtgata aagtagatac atcaacaaag
     1141 gtagaaaata attcaacatt taatatatcc aatgttgaat tgatatataa tgaatatgat
     1201 gagaataaaa aaattatatc ttcggatagt aaggctttgt tggatatgac tttaatgcct
     1261 gggaaagttg cttacataga atgtgggcat aaaacttttg taaagggtgt agaagtttat
     1321 gcatatgaat atgaggcaga gggtaagatt gtgtatgtta atttaaaaga aaatactatt
     1381 aatataagaa ataataatat taagctagaa aatagtagtc agtatgaggt gctatctaca
     1441 tcagaactta aaaaagtaaa tgaatcaaat gagggaatta cgtatcaagt aaaagtaaaa
     1501 aattcatcat caaaagattt aggaaatatt attcttaaga cagcagaagt taatgaaaat
     1561 ggagaatatt taacagtaaa tcgtgtacca tcatataaga ttttaaagcc ttcagaagag
     1621 acagatatag atatattgtg ttcaacaaag gcaaaaagtg ttgaaatagt gggatatacg
     1681 tatgatgata taaaagaaaa agccaatgtg gatattgact taaaatctca taaagttaag
     1741 atagacaaat aaaggtaggg gatacttatg aaggttttaa tagttgaaga taataagata
     1801 cttttagaaa gtgtagtaga agaacttagc aagcattttg aaactgaaaa gtgtgaagat
     1861 ggagaagaag ctttatactt agtaaatcaa aatatatatg atttagttat tttagactta
     1921 atgcttccta atataaatgg atttgatatc ctaaagaaaa tgagagttaa taatatagat
     1981 acacctgtac ttatattgac agcaaaagaa acattagatg acaaagtaga agcatttact
     2041 ataggagcta atgattattt gacaaaacct ttttatatgg aagagttagt agctagggta
     2101 tacgctatat taaggactaa tggaaagata aaagaaagaa acggattaga atttaaatct
     2161 ttatatttag atacactaga aaaaagagtg tatattgaaa aagaagaaat aaaacttcaa
     2221 aacaaacagt ttaacttgct tgagtatttt gtattaaata aaggttcaat tttattaaaa
     2281 gagcagattt atgatagaat atggggaatt gattcagatg caactattga aatagtggaa
     2341 gtatatgtaa gtaatctaag aaaaaagcta agtaaatatg gatatgataa atatattaaa
     2401 actaaacgta aggtaggata tatattcgat gataaataag aatgtattta ccagtactaa
     2461 aaatcattta ataaaaatgt atattattgt agtaggtagt tttttaataa tcttttctat
     2521 atttatatac tcatatttta ggggacttac atatagtgga attgatagtg agataaatga
     2581 tgaacttgaa tatatagtct ctcaatttaa aagaacttca tttttgaacc caataagact
     2641 gaaagatcct aaagatatgg tgtatgtata tgaagatgga aggataagtt actatactca
     2701 aaatgaatat tttgatgagt tacttccaga tagaagacta gataaaaaaa attcgttttt
     2761 taaatacaca gaaaatggat atacttttag agaattaaat gtagatgtag gtagatacca
     2821 aatacagata attagaaata tagattctga gatgaattct ttaagacagc ttacatctgt
     2881 tcttataatt ggaatattaa tttctgtaat aattacttac tttgtggctg tatatctcac
     2941 tagaaaagct ttaattccaa tagaaacagc atggaaaaat caagctaagt ttatacaaga
     3001 tgcttctcat gaattaagaa caccaataac aatagtttca tcaaaattag aaagtatgtt
     3061 aaaatcccca gaaagtacag taaatgatga agttgaaaca atagctactg caatgaagga
     3121 aactagaaga cttaaaaaaa tgataactga tttattgtct cttactaaag aggactctat
     3181 tgttaaagtt aatttagaag aaatagactt agaaaaatta ttggaagaaa tatcagaaga
     3241 ctatattgac attgctgagt ttcaggaaaa aagattcgtg tttaattcta aactgaagaa
     3301 taaagttata ataacagata aaaataaatt aagacagctt atattaattt tcattgataa
     3361 tgcctttaaa tatacaaaat tgggtgatga gatagcatta gaattaaaag aagatataga
     3421 ggatgaggta actttactta taagtgacac tggtataggc ataaaaaaag aagagatacc
     3481 tcttatattt gatagatttt ttagaagtga aaatgtaaga aataaagatt tagaaggttc
     3541 tggaatagga ctttcaatag ctcgcatgat tagtttaaac ttatctattg atataaatgt
     3601 aacttctgat gtagatattg gaactacatt tgaattgtca ataccaaaaa agttaaaata
     3661 ataaaaatat aaaaaattgt ttttagaaat gaagaatggg tatactaatt agtataaaat
     3721 ataaaaagac cttataaaag taaatttata tttataaatt tcatagtata attatatagg
     3781 gataataaag tttaatagga ggatttaaaa atggcaaaag ttataaatac atctgaattt
     3841 agaagtagtg ttgaaggtag taagggagta gttgtagttg atttctttgc aacttggtgt
     3901 ggaccttgta acatgttggg acctgtattt gcagaattag gtgaagagat gaaggataaa
     3961 gctagatttg taaaagttga tatagatgag agcttagaaa tagctcaaca attcaatgta
     4021 tctacagtac caactatgat tatatttaaa gatggaaaac cagtagaaac tttaattgga
     4081 ttcatgccaa agaacaaaat tgaaatgcaa gtaaaatcat atttataaaa aatgggtgag
     4141 tattatgaga tatgatatag ctataatcgg cagtggtcca gctggattgt ctgctgctat
     4201 aaatgcaaaa ataagaaata aaacaattat catgtttggt aatgataatt taagtaataa
     4261 gcttgttaaa gctccttcaa tagataatta tttaggtttt tatgatataa gtggtgatga
     4321 attaaaagac aagtttaaat cacatataga tagtatggat atttcaattg aaaataaaag
     4381 aataaataat atttatgcta tgggagaata ctttacaata atgtctggaa atgatatgta
     4441 tgaagctact actgtaatcc ttgcaactgg ggtagagtat actagaccaa tcaagggtga
     4501 agaagaattt ctaggaagag gtgtaggcta ctgtgctaca tgtgatgcac cactttatag
     4561 aaataaaaag gtagcagtta taggatataa tgaagaatct aaagaagaag caaatttttt
     4621 aagtgaactc acttcaaaga cttattttat acctatgtac aaaaaagata atcttatgag
     4681 aagttcagat aacctagatg attctattga agttatacat gacagaccag tacagataga
     4741 tggagataaa cttgtaaaca aagtatcttt taaagaaaac cacattgaag ttgatggagt
     4801 attcgttata aaagatagta cggctccaag tgcattggtt cctggaatag agattgatgg
     4861 cattcatatt aaagttgaca ataatatgaa gactagtata gatggatgct ttgctgctgg
     4921 agattgtgtt ggaaaaccct attcttatat aaaagcagct ggtcaagggc aaatagcagc
     4981 tttaaatgct gtttactatt tagataaatt aaagagggct taatatttat attgattaac
     5041 tttttaaaga tatttataaa aaactgtctt gaaa
//
LOCUS       DAGQWR010000215         5019 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.218, whole genome shotgun sequence.
ACCESSION   DAGQWR010000215 DAGQWR010000000
VERSION     DAGQWR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.218"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..300)
                     /locus_tag="MIW95_003684"
     CDS             complement(<1..300)
                     /locus_tag="MIW95_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBY3152978.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIRFVFKEFLSFVSFRLLSGVVEMLIMYVMIDL"
     gene            527..2938
                     /locus_tag="MIW95_003685"
     CDS             527..2938
                     /locus_tag="MIW95_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBY3152979.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISIGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            3235..4812
                     /locus_tag="MIW95_003686"
     CDS             3235..4812
                     /locus_tag="MIW95_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBY3152980.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVENLEAKVDSIEKAIDSISQKVN
                     KIQDILDKIGFLKKFLS"
BASE COUNT         2058 a          613 c          873 g         1475 t
ORIGIN      
        1 caaatcaatc attacataca taattaacat ttcaacgaca ccagaaagta gcctaaaact
       61 aacaaaagac aaaaattctt taaatacgaa ccttatttca attcttttac tttcaaatac
      121 aaaaaactta ttagtaacat aagcaaataa aacagcaagt atccatgcca aagcatttgc
      181 aaccataaaa ttaaataaaa taactctagt aaaaaataaa tacgaaacta tatttacaag
      241 agtagtaaat gctccaaaaa ataaatataa tattgtctct ctatgctttt ttaaaatcaa
      301 atataatcct cctaatctaa tttaatataa atatacttaa agatactaac aaacattagt
      361 atagcacatc tattaataaa tcaatatatc ttaaaatatt ataaaaataa aactataata
      421 ttctataatt aatatgtact cataatccat attttatcaa aataaaatat gaccttaatt
      481 tagaaatctg atataataaa aattaaatac atataagggg gtaaacatga gaaagtacaa
      541 atcaaaaaaa ttgtcaaagc tactggcatt gttgacagtt tgctttttaa tagtgtcaac
      601 aatacctgtc tcagcagaaa accataaaac tctagatgga gtagaaactg cagagtattc
      661 tgaaagttat cttcaatacc tagaagatgt caaaaatgga gacacagcaa aatataatgg
      721 agtaatacct tttccacatg aaatggaagg tacaacactt cgaaataagg gcagaagtag
      781 tcttccatca gcatataaat caagtgtagc atacaaccca atggatcttg gtcttactac
      841 accagcaaaa aatcaaggaa gtcttaatac atgctggtct ttttcaggta tgtcaacttt
      901 agaagcatat cttaaattaa agggatatgg aacgtatgac ttatcagaag aacatttaag
      961 atggtgggca actggtggaa aatatggatg gaacttagat gatatgtcag gtagttcaaa
     1021 tgtaacagcc ataggatatc tgacagcatg ggcaggtcct aagttagaaa aagatatacc
     1081 atataatctt aaatctgagg cacaaggtgc aactaaacct tcaaatatgg atactgcacc
     1141 tactcaattt aatgtaacag atgttgttcg tcttaataaa gataaggaaa ctgtaaaaaa
     1201 tgctataatg caatatggtt cagtgacatc aggttatgca cattattcga catattttaa
     1261 taaagatgaa acagcatata actgtacaaa taagagggct ccattaaatc acgctgtagc
     1321 gatagtagga tgggatgata attattcaaa agataacttt gcatctgatg taaaaccaga
     1381 atcaaatgga gcatggttag taaaaagcag ttggggagaa tttaattcta tgaaaggatt
     1441 cttctggatt tcttatgaag ataaaactct actaacagat acagataact atgcaatgaa
     1501 atcagtatcg aaaccagata gtgataaaaa aatgtaccaa cttgaatatg ctggtcttag
     1561 taagataatg tcaaataaag taacagcagc aaatgtattt gattttagca gagattctga
     1621 aaaacttgac tctgttatgt ttgaaacaga ctctgtagga gcaaaatatg aagtatatta
     1681 tgcaccagta gtaaatggag ttcctcaaaa caactcaatg acaaaacttg caagtggaac
     1741 agtatcatat tctggataca taaatgtacc tactaattct tacagcttac caaaaggtaa
     1801 aggagcaata gtagtagtta tagacaacac agcaaatcct aatagagaaa aatcaacttt
     1861 agcatatgaa actgatatag atggatacta tctatatgag gctaaagcaa acttaggtga
     1921 aagctacata cttcaaaaca ataagtttga agatataaat acatatagtg aattttctcc
     1981 ttgtaacttt gttataaaag ctataacaaa aacatcttct ggacaagcta cttcaggaga
     2041 atctttaact ggagcagata gatatgaaac ggcagttaaa gttagtcaaa aaggatggac
     2101 ttcttcacaa aatgcagtat tagtaaatgg agacgcaata gtggatgctt taacagctac
     2161 accatttaca gcagcaatcg actctccaat tcttttaaca ggaaaagata acttagattc
     2221 aaaaactaag gcagagttac aaagattagg aactaaaaaa gtttatctaa taggtggaga
     2281 aaattcttta agcaagaatg tacaaactca acttagtaat atgggtatat cagtagaaag
     2341 aatttcaggt agtgatagat acaagactag tatatctcta gctcaaaagt taaatagtat
     2401 aaaatctgtt tcacaagttg cagtggcaaa tggtgtaaat ggacttgcag atgcaataag
     2461 tattggtgca gcagctgctg ataacaatat gccaataata cttactaatg aaaagagtga
     2521 gttgcaaggt gctgatgagt ttttaaattc atcaaaaata actaagtctt atataattgg
     2581 tggtacagct actttatcat caaatttaga aagtaagctt tcaaatccaa caagacttgc
     2641 aggaagtaat agaaatgaaa ctaatgctaa gataatagat aaattctacc ctagctcaga
     2701 tttgaaatat gcttttgttg ttaaagatgg ttcaaagagt caaggagact tgatagatgg
     2761 actagcagta ggagcattag gtgctaaaac tgattcacca gtagttctag ttggaaataa
     2821 gttagatgaa agtcaaaaaa atgtacttaa gtctaagaaa atagaaactc ctattagagt
     2881 tggtggaaat ggaaatgaaa gtgcttttaa tgaactaaat actcttttag gaaaatagtg
     2941 aatggaaaga tagtaaggat atacatttta ttattaaaaa atattcaata gaagtttata
     3001 aatataaaat aacaaaaagg ggtgtctcaa aatgaacttt ttagttcatg aggcactctt
     3061 ttttgttgtt ttatattttt atcatttaaa taatgaaaaa taggcttgtc tatattttta
     3121 gatagttttc ttattattga tgaaaaatgt aataatttta aactgtaaaa tagtgtaaag
     3181 ttattttata atataaatat agtaagattt taaacaaaat aagggggaat aataatgaaa
     3241 gcaccaaaaa ctattttaac aatactgaca atagcgctta ctttaagtag catttctata
     3301 ataccatcat atgcacttac agaggaaaaa ttaataggta atggtagata tgaaactgct
     3361 gtaaagataa gtcaaaaggc atacagttct agttctaatg tagtgttagt taatgacaat
     3421 tcattagctg atgcactttc agctacacca tttgcaaagg ctaaaggagc accaatactt
     3481 ttgacagaaa gtgataagct agatgatagg actgaaaaag agataaaacg tctaggagct
     3541 aaagatattt atttgatagg aggtactgct gtacttaata aagatataga gaacaagtta
     3601 aaaggtaatg gacttaatgt agaaagaatt aatggtaagg atagatatga aacttcactg
     3661 attttggcta ataagcttaa ggatataaaa gatattaagg aagttgctgt agtaaatggt
     3721 gaaaaaggat taagtgatgc tgttagtgtt ggagcaccag cagctcaaaa taagatgcct
     3781 ataattttgt ctagtcctaa ggacggagta gaagcatttg ataaatttat aagagatgag
     3841 aaagttataa aagcatacgt aattggagga acaaattctg tttcaagagc tgtagaaaag
     3901 agccttccta atgcagaaag agtgagtgga aaagatagaa atgagactaa tgccaaagtt
     3961 atagaaaagt tttatactga tacaaattta agtaatttat atgtaactaa ggatggtagt
     4021 aaaaatgaga atcaactaat agactcgtta gctgttggag tattggctgc aaaaaatgag
     4081 tctcctgttg ttttggttgg taataaatta aacacgaaac aaagggatat acttagtact
     4141 aaaaaattaa atactataac acaggtaggt ggaaatggaa atgaagaagc ttttgatgaa
     4201 attaagagtt tacaagaaaa aactgtgttt gaagcaaaaa ctgttgagga attaactgat
     4261 atgataaata tagcaagtcc taatgatatt ataaacttta aaccaaaaga gaatactgta
     4321 aatgaagctt ttagaatggt aacaaataaa ccaataactg tgaatataaa gggtgattgt
     4381 tcaaagactc ttacagtaga tatgcctaat ggtgaagtga ataactatgc tactttggta
     4441 aatgttatag ttagaaatat tggagaaggt ggatttaata accatgatac aatcactata
     4501 ttgtcagttc gtgacaaaaa tggtagagtt atagaaaata ctagaaattc agatatagat
     4561 acgttgatga tattagcttc tgctaatgat acaaaattga ttaatgatgg atatatagga
     4621 aagcttatag ataattcatc taatagtgat ataactaata atggtactat agataaaaaa
     4681 gttaatcaag ttgagaattt agaggctaaa gtagattcaa ttgaaaaagc tatagattct
     4741 ataagtcaaa aagtcaataa aatacaagat atactagata agattggatt tctaaagaaa
     4801 tttcttagtt aagaatatgt atatttaagt gaagtgtgtg ttgtaattgt tctaaagtta
     4861 tatagttaaa caggtttata tacaaacaga cttatagata aatatgtagt ttagtatata
     4921 tttgaataag ctttggggag gtaacatggg gattggaatt gaatgtaaca aaaaaataaa
     4981 taaaagtatt gacaaaacta gaataaagaa aaataatct
//
LOCUS       DAGQWR010000216         4971 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.219, whole genome shotgun sequence.
ACCESSION   DAGQWR010000216 DAGQWR010000000
VERSION     DAGQWR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.219"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            288..977
                     /locus_tag="MIW95_003687"
     CDS             288..977
                     /locus_tag="MIW95_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3152981.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYFVKTAVDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            977..1894
                     /locus_tag="MIW95_003688"
     CDS             977..1894
                     /locus_tag="MIW95_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY3152982.1"
                     /translation="MELFLVVISIVLFAFYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATLEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            1967..2893
                     /locus_tag="MIW95_003689"
     CDS             1967..2893
                     /locus_tag="MIW95_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3152983.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNDVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     KNQIGINSIGRKQSSLEDYFFQTTGTGGIETL"
     gene            2890..3570
                     /locus_tag="MIW95_003690"
     CDS             2890..3570
                     /locus_tag="MIW95_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3152984.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSILGILLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLVFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            4015..4716
                     /locus_tag="MIW95_003691"
     CDS             4015..4716
                     /locus_tag="MIW95_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBY3152985.1"
                     /translation="MNNSFLDKLIETKELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGRHTLDDVQETVNSSYVFDTVIDSLKEKITHNFLRNLHSSLIFNTTLH
                     SRGMAGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEDLYRNSLNSCSLGNYVPLIEYL
                     SSLEDFREVYKMLWK"
BASE COUNT         1906 a          524 c          787 g         1754 t
ORIGIN      
        1 tttctttttt atgtataaaa tattttttag caaaagttaa aaggtgtttt ataatataaa
       61 atagttaatt gtagatgtta ataaaagaaa attatgtata tttcagataa agataagaga
      121 gtatactata ttttaattat tttttcaata aaacttattg aaaagaactt taacttagaa
      181 gagtatcacc aaattgcaga tacaatatca taagcagtta tttattgaca acccttttta
      241 ataaatatat catttgaagt atactgacga aaaggagttg aataataatg gacatcttat
      301 tggttgaaga tgataagaat attcgagaaa tggtatacga ttttctgatt agtgagaatt
      361 attttgttaa aactgccgtt gacggaaata cagctattga aatatttaaa aattattcat
      421 ttgatattat tttactggac ttgatgttac caaagtctag tggttttgaa gtattaaaat
      481 atatacgaag tgtgagtgtt gttcctgtta ttattactac tgcgaaagat agtgacacag
      541 ataaaatgct agggcttaat cttggtgcag atgattatat tacaaagcct ttttcaatcg
      601 tagaattgct tgcaagaatt aaagctaata ttagacgtgc aacggtgtat agcagttcaa
      661 gaaactctga agatatggtt attcagtata aagatttgat tataaacata tcaaaatatg
      721 tagtttcaaa gaatggaatt gacttaaact taacacatac agaatttgaa attgttaaat
      781 tactagcgac taatcaagga cgtgcttttt caaaggaaca attatacaac actatttgga
      841 aagaacctta ttatggaaat gaaaatgtgc taaatactca tattaacagg ttaagaaata
      901 aattgtctga tggtatagaa aaaaataatt atattaaaac tctttgggga attggctaca
      961 aaatggagga agaataatgg agttattttt agttgtaatt tcgattgtac tatttgcttt
     1021 ctacttaaaa gaacgaaata agtcaaagaa aatggaaagt gattataagt atatcaattc
     1081 aagacttcgt gatataacaa atgatgatga aattaattat attttagtac catctgatat
     1141 ttcgattgta aaagaaacag cagtagatat aaataaactt cttgaaaagt tttacagtag
     1201 gcaaattaat tataatagaa gtgaaaaagc gatattgcaa atatttacaa acatatcaca
     1261 cgatttacga actcccatca cagtattaaa gggttatatt gaaatgctat atcttcaaag
     1321 tcaaaaagaa gaattatctt cttcaatgag agcaactttg gaaaaaatgc aaacaaattc
     1381 aaatgagctt gttaattcaa taaataacct gtttaatatg gcaaaaatcc aatctggaga
     1441 tatgatatta aatcttcaaa aatccaatct aacacaatta tgtcatgaga ttattttaga
     1501 gttttatgat ttactagata aagaaaactt taatgttgaa gttaatattg aagatagacc
     1561 tatatatgca aatgttgaca cagaagcatt acaacgtata ctcaaaaatt taattgataa
     1621 tgctataaaa tatggttcag atggtagatt tttagggatt tcactatatg aaaaggaaaa
     1681 acatgttttt atagatattg aggataaagg ttcaggtatt tctgaaactg aaaaagagca
     1741 tattttcaca agagcttata ctgctgacag aaaaaaaggc aatggtttag gtttggctat
     1801 ttctcaaggg cttgcaaact ctatgggagg ttcaatttat gtaaatagta gacctaatgt
     1861 aaaaacagtt tttacggtaa aattgaaaag ttagaaaaaa gttagattta aaagagagaa
     1921 aagataattt tattttgtat actgttttta gaaaggaggg agtatgatga actatatcat
     1981 agagacccat caattaaaga aaatatataa agataaagct gttgttaatg atgttaatat
     2041 tcatgtgaaa aagggagaaa tttatggatt tgttggacca aatggagcag gtaaaagtac
     2101 agtaatgaaa atgttgctaa atctcgttaa gccaaattct ggagaaatag ttatgttcag
     2161 taagaaagtg gcagaaactg attttgaaat attaaaaaag attggtacta ttattgaaaa
     2221 tccttatttc tatgagaatt tgactgctaa acagaatctt gatttacact gtgaatatat
     2281 gggttattat aacaaagaac atatatcaga ggtattggaa tgtgtcggat tgtcaaagca
     2341 atcaaacaag aaagtttcaa aatattcttt agggatgaaa caacgtcttg caatagctag
     2401 agcaattctt actaaaccag aattattgat tcttgatgaa cctataaatg cactcgaccc
     2461 agaggggata agagaaatgc gagaattatt ccgtaagtta aatacagatt atggaataac
     2521 tatatttatt tctagtcata ttctttcaga ggtagaacaa attgcggaca ctattggaat
     2581 tattcaagat ggtaaactta ttaaagaaat ttctatgtca gatatacaca aatatcaaac
     2641 agattatgtt gaagtagatg tagataatgt ggaacttgct ggatacttgt tagaaaaaga
     2701 atttggaata aaagacttta aaattacttc ggaaagttgt attgaaatat atgatttacg
     2761 aaaagatgta aaagaaatat ccaaaatatt tatcaaaaat caaattggta taaatagcat
     2821 tggacgtaaa caaagttctt tagaagatta cttcttccaa actacaggta caggaggtat
     2881 agaaacacta tgatacattt aatgaaacta gaattaaaaa aagttgggct aaaaagatat
     2941 tttttatttt caatactggg gattttgtta tgtatgtttt ttgtctttac tggattgaat
     3001 gataaatcta cttcaatata tgattatgat gttacattta ggacaatcag tttagttttc
     3061 tgtttttact atattatatt atttgctgtg ttgaatgtgg catatataat aaatgaatat
     3121 acaaataaaa caatcttgat tttattctct tatccacttg accgtaaaaa acttatttta
     3181 tcaaagttac ttttgattac gactcttatg atatcatcaa tgatagttgg atatatctgt
     3241 tgttttacat tcgttgttgt gttggataag tattttaatt tagttattgg agattttcat
     3301 atttcaattt tatcttattg gataccagga gcaattaaaa gtattattgt attttgctca
     3361 ttaggtatat ggacattcgt tgttggaatg ataagaaaat caatacctgt tacccttgtc
     3421 agttcaatta tttttatcaa tttaagacaa tttattttag ctggtacaaa tacaactcaa
     3481 gacagtctga tttctgtttt tattatagtt ggaataactg tgattggtat atattatata
     3541 ctaaatcata aagttactga aattgactag atactgtgga taggaaggta gttattaatt
     3601 gtatcttctt gggataataa tgatttgcta ttatatcagc tattattaat attattagag
     3661 ttttaataat attaataata gctatatatt ttttcttctt atttataaat ttaatgattt
     3721 atagtcataa aatgaactct aagaagagat aaaaatgcgt tttatatgtt tttagattaa
     3781 tatgaaatat cacaatgaaa tgttgtacaa agtgatatat aataattata tgaactataa
     3841 aattgactaa acttattgac taataaatta gatgaaaagt tgagacttac tgaaaataaa
     3901 tacaatgaaa tatatactac aagtattaaa ttaaatctga actttacata ctattagaat
     3961 aagtattata agattataat gatttaaaaa tattaaaata attgaggtga taacatgaac
     4021 aattcctttt tagataaatt gattgagaca aaagaactta aaaattcact ttataatgtg
     4081 ttaaaacaca attttcttta tcattctaat aagatagagg gcagtacttt tactactgaa
     4141 gcattagcac ttttactcga taagaatgtt gttacaggaa gacatacttt agatgatgta
     4201 caagaaactg ttaattctag ttatgtattt gatacagtaa ttgactcact gaaagagaaa
     4261 ataacacata atttcttaag gaatctacat tcaagtttaa tcttcaatac aaccctacat
     4321 tccagaggga tggctggtat atataaaact atacccaata tgatactagg aactgatgta
     4381 agcatagcac aaccatttga agttgagcca aagcttgatg aattaattga atggtactat
     4441 agtcagagtg aagtgtctat taaagttata gctgaatttc attatagatt tgaattgata
     4501 catccatttc aagatggtaa tggtcgcata ggtagatttg ttatgctaaa acagatgtta
     4561 gaaaataatc ttcctataaa aattgtatct tgggatagtg aggacttgta tagaaactcc
     4621 ttaaattctt gtagtttagg taattatgtc cctttaattg aatatttatc aagtttagaa
     4681 gattttagag aagtatataa aatgttatgg aaataattgt ttgtagcaaa aaaagttttt
     4741 atataaatat atttttaact atgctattta acgttgattt cttagttatt cttagcttgg
     4801 actataccca ctattttaac tatataaaat agtggatata gtttaactat ctctttataa
     4861 ctatttttat gtatcttagt tgtttcttaa tattgggaca gttcataaaa ctaaaagtga
     4921 tttttaattt tatcatttat aatttaccat tacatcagaa aaatgcttat c
//
LOCUS       DAGQWR010000217         4937 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.220, whole genome shotgun sequence.
ACCESSION   DAGQWR010000217 DAGQWR010000000
VERSION     DAGQWR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.220"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            82..906
                     /locus_tag="MIW95_003692"
     CDS             82..906
                     /locus_tag="MIW95_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBY3152986.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            1035..2300
                     /gene="gluD"
                     /locus_tag="MIW95_003693"
     CDS             1035..2300
                     /gene="gluD"
                     /locus_tag="MIW95_003693"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBY3152987.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            2493..3149
                     /locus_tag="MIW95_003694"
     CDS             2493..3149
                     /locus_tag="MIW95_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBY3152988.1"
                     /translation="MYFLPPHDYKLEELDWIFGHELMYFKSKDLHLKYLVLFLKIVYW
                     FNPFIYIMEKYMDLDCELYCDERVLKNCTVQQKQEYALTIVNSMKRGINFSNKFVTGL
                     NKQSDIRKRVMNMFSEKHKNGILMALIISLFSSITFFKFDSTSLIEENDFLERSRYAF
                     EGRQEDMFVTIDSTYADAPIKLRKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK
                     "
     gene            3480..4613
                     /locus_tag="MIW95_003695"
     CDS             3480..4613
                     /locus_tag="MIW95_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBY3152989.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGVEYYDSLLND"
BASE COUNT         1995 a          460 c          837 g         1645 t
ORIGIN      
        1 taatgatata ataaatttaa tctataaagt aaataaaatt ttgtaataaa tagttaaagc
       61 tattttataa ggagatatat gatggaagta tatgctttgg ttgggtcaag tggcacaggg
      121 aagagcttta aggctttaga gttcgcatat gaaaatgata tagaatatat aatcgatgat
      181 gggattttaa tatacaaaaa caaggtttta gctgggatat cggcaaaaca agcaaatact
      241 actatagaag cagttaaaag ggctattttt tataatctgg agcatagaca agaagttaga
      301 gaagcaatta aaaaagaaaa tataagaaaa attcttatta ttgggacatc aaaaaaaatg
      361 gttaatcaaa ttgtagagag attgtcaatt ggaagggtct gtaagtttat aaatatacaa
      421 gatataagta ctaaatatga aatagaaata gcaaaacaag ctagaaaaga aggaaatcat
      481 gtaattcctg ttccagccgt tgagataaaa agtatggcaa gtggtctcag tataaattct
      541 ttgaagagac tttttagaaa aggaaataat aggaatatgg tagttttaga aaaaacaatt
      601 ataagaccaa cttttagtta tataggaaag ttttatatta gtgctgatgt aattaaacag
      661 attattgaat atgaagtata caactttgga agcattgata gaataaataa aatcaatata
      721 gagaattgca ataattttat gaatattttt gtaagtataa atatcaatga tttagaaact
      781 ataaaaaaaa ttgaagacat acaacagaca ataaaaaagt ctattgagaa aatgacttta
      841 gttaatgtac aaaaaataga tattaacatt cataaaatta aaaaaataaa cttcctccaa
      901 tgttaaaaaa taaactgaga aaatgatata ctaatttgca ataaagtgtg ctataattaa
      961 actgtaaatt taggaaatgg ttttttctaa atggagtata cataattata acgtatttat
     1021 aagggggctt tcaaatgtca ggaaaagatg taaatgtctt cgagatggcg caatctcaag
     1081 taaaaaatgc atgtgataaa ttaggtatgg aaccagcagt ttatgaatta ttaaaagaac
     1141 ctatgagagt tatagaggtt tcaattccag taaaaatgga tgatggttct ataaaaactt
     1201 ttaaaggatt tagatcacaa cataatgatg cagtagggcc aacaaaaggt ggaataagat
     1261 tccatcaaaa tgtttcaaga gacgaagtaa aagctttatc tatatggatg actttcaagt
     1321 gttctgtaac aggtatacca tatggtggag gtaaaggtgg aataatagta gatccatcaa
     1381 ctttatctca aggtgaatta gaaagattaa gtagaggata catagatgga atatataaat
     1441 taataggtga aaaagttgac gttcctgcac cagacgtaaa tactaatggt caaataatgt
     1501 cttggatggt tgatgagtac aataaattaa ctggacaaag ttctataggt gttataactg
     1561 gtaagccagt tgaatttggt ggttctttag gaagaacagc tgctactgga tttggtgttg
     1621 ctgttactgc aagagaagct gctgctaaat taggaataga catgaaaaaa gctaaaatag
     1681 ctgttcaagg tataggaaac gttggttctt atacagttct taactgtgaa aaacttggtg
     1741 gtactgttgt agctatggct gaatggtgta aatcagaagg ttcttatgct atatacaatg
     1801 aaaatggttt agatggtcaa gctatgttag attatatgaa agaacatggt aacttattaa
     1861 acttcccagg agctaagaga atatctttag aagagttctg ggcttcagat gttgatatag
     1921 taataccagc tgcattagaa aactctataa ctaaagaagt tgctgaatct ataaaagcta
     1981 aattagtttg tgaggctgct aatggaccaa ctactccaga ggctgacgaa gtatttgctg
     2041 aaagaggaat agttcttact ccagatatat taactaatgc tggtggagtt acagtttctt
     2101 acttcgagtg ggtacaaaac ttatatggat actactggtc agaagaagaa gtagagcaaa
     2161 aagaagaaat agctatggtt aaagcatttg aatctatttg gaaaatcaaa gaagagtaca
     2221 atgttacaat gagagaagct gcttacatgc attcaattaa gaaagttgct gaagctatga
     2281 aattaagagg atggtactaa gatttactct taataaataa taggatataa ttatttaaga
     2341 tataaagggg ctgtacatag tacagccctt aaattatgtg gcttacaatg aagtaagcta
     2401 tttttgtttt aacttataaa taacttgata aattatgtaa tttagaatgc ataaataaga
     2461 tgggtgaaaa ttaatggttg atttaatttt gaatgtattt tttaccacca catgattaca
     2521 aattagagga gttagattgg atttttggac atgaacttat gtattttaaa agcaaagacc
     2581 ttcatttaaa gtatctagta ttatttttaa aaatagtata ttggtttaat ccatttatat
     2641 atataatgga aaaatatatg gatttggatt gtgaacttta ttgtgatgag agagttttaa
     2701 aaaattgtac tgtacaacaa aagcaagaat atgctctaac tattgtaaat tcaatgaaaa
     2761 gaggtataaa tttttcaaat aaatttgtaa ctggattaaa taaacaatct gatattagaa
     2821 aaagagtaat gaacatgttc agtgaaaaac ataaaaatgg tatcttaatg gctttgatta
     2881 taagcttgtt ttcttctatt acatttttta aattcgattc aactagtctt attgaagaga
     2941 atgatttctt agagaggtca agatatgcgt ttgaaggaag gcaagaagac atgtttgtaa
     3001 caatagattc tacatatgca gatgcaccta taaaacttag aaaagagcat gaagaaaact
     3061 gtagagtcaa tggaatagta ccaaaagatt cagattcaat agaaattatt cctagatttt
     3121 ataaagattg tatggattta aaaaaataag ttagacttaa tattgatgtt gatattatga
     3181 attttttata tttaagcaat tgaaaatttt attatagatt caattttata tgtgtttaag
     3241 tatgaaattt atagatagtt gaaataatta aatatgattg atgcatagga atcattacta
     3301 agctataatt aattataggg gagtttaaat aaattatgag ttacacaatt ataaaatgat
     3361 aaaaaatagt tatctgttac aaaaaatgta tctttttaat taataaagat aatatgaaaa
     3421 cttaaaaggc aaataaactt aaacataaaa tactcttaaa taagatagga gaaaaatgta
     3481 tgtctaactt tattctaaat gggattttta gaacagttat agtaagtagt ctaagcattc
     3541 ttctgatatt gattttcaag aaaaatgtat ttaaaagatt tagcaaaaaa tttaattatt
     3601 atatatggat gatagttgtt attaaattat ttttaccatt tacatattat acttttacaa
     3661 taaatatctt gagaagtaaa aaacacatta atattaataa cataaattta gagggattta
     3721 ataatgtttc aactataagt aataatatta tattgtacac ttggattata actgtagtag
     3781 tgtatttaat ttacactata tttaaatata taaaacttaa aaatttaata aatgacttat
     3841 cttatgatgt tgatgataga gagattgtaa atctttataa aaatatatta gaagaattta
     3901 atataacaaa agatataaaa ctcaaatact cttatgaagt tgaaacacca gcatttttta
     3961 attcatgtgt acttttgcca ccacatgagt ataaattgaa agagttagac tggattttta
     4021 gacatgagct tatgcatttt aagagtaggg acctttattt aaaatatgta atcctgtttt
     4081 taaaaacagt atattggttt aatccattta tatatataat ggataaacat atagatttag
     4141 attgtgaact ttattgtgat gaaagagttt taaaaaatcg aaattcagag gaaaaaaagg
     4201 attatgcttt aacaattcta aatgcaatga gaaaaggttc aaatacttca aataaattta
     4261 tagctggact ccataagcaa tctgatatta aaaagagagt tatgaatatg tttaatgaaa
     4321 agtacaagaa tggtatatta atggcattaa ctttatgttt attatcttct attacttttt
     4381 tgaaggttaa ttcaacgagt attgttaatc ttaatgtatt acctaaagga tttgaagact
     4441 tacgaactgt tagcagtata cgtttttcag ttataggatt tacatatgca gatgctccta
     4501 aaaatatgag ggaagaatat aaaataaatt gtgaatcatt aggtttgata cctaaagact
     4561 ctgataaaat agaagttggt gtagaatatt atgacagtct attgaatgat taaataaata
     4621 ttattttaac agatattaat tataaagttt tgaatagtaa tttgttgaga aataaatata
     4681 tccttataaa aatagatact tatataccaa gaataaaaaa tagttaagat agtacacaag
     4741 gatatactta taaagaactt aaataagtga aataaattat taattataag gtttataatt
     4801 ttaaaaaatt agataaattc aggtgtaaaa tactataaaa cacaattcgg aaaagtgtat
     4861 gttgaacttc atttaaaata gaattttcaa agttgttata gtaagtagtt taagcattct
     4921 tcttatatta atctttg
//
LOCUS       DAGQWR010000218         4836 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.221, whole genome shotgun sequence.
ACCESSION   DAGQWR010000218 DAGQWR010000000
VERSION     DAGQWR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.221"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     misc_binding    149..380
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            488..1969
                     /locus_tag="MIW95_003696"
     CDS             488..1969
                     /locus_tag="MIW95_003696"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBY3152990.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKKFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            1979..2872
                     /locus_tag="MIW95_003697"
     CDS             1979..2872
                     /locus_tag="MIW95_003697"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBY3152991.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIHSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQNYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            2996..4057
                     /locus_tag="MIW95_003698"
     CDS             2996..4057
                     /locus_tag="MIW95_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBY3152992.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4212..4724)
                     /locus_tag="MIW95_003699"
     CDS             complement(4212..4724)
                     /locus_tag="MIW95_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBY3152993.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKIYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
BASE COUNT         1798 a          599 c          758 g         1681 t
ORIGIN      
        1 cttttaaaaa agttaaaaaa atatttttct aatactcaat ttgattacaa tttttttaca
       61 atttaaaaaa tattttttcc aaatttttat attaatactt gactttgtct gttttataaa
      121 ctagaataat taataatatt aagaatattg aaaggcattg attaggaaga gtaaatctga
      181 atcgattacc agagaaggaa actactagct gaaatgtttc cctaattaga aggattgaaa
      241 actacctagg agcttctatc cgaagttatc ttaatgattt tagtaggaat agacgttttt
      301 attacttgcg ttaaagtatc aagtggattt tttaaaatca atttgggtgg aaccgcggga
      361 aacagattct cgtccctttt ttagggatga gggtcttttt ttattaccta aattcggttt
      421 ttaaaatatt ctgtatctaa tttaattagc taattaaaaa tttaaaatat atggaggatt
      481 ataaaaaatg tattattcaa gtacgagagg caccgaagaa aaagtaactg catcacaagc
      541 aataataaaa ggaatatcaa gtgatggtgg tctttatgtt ccaagtgaat ttcccaatgt
      601 aaaaaatgaa cttataaatt tagtaaattt aacgtattct cagatagcat tttttgtact
      661 tagtaaattt ttatgtgatt ttactgaaga tgaaattaaa aattgtatag aaaatgcata
      721 tgatgaaaag tttgattgta gtagtatagc tccattaaat aaagtaaatg atacttattt
      781 cttagaacta tatcacggtc caactttagc atttaaagat atggcactta ctataatgcc
      841 tcatcttctt aaaacatcta taaagaaaga taatttagag aaagatgttg tgattcttac
      901 tgctacatct ggagatacag gcaaagctgc acttgaggga tttaaagata tagataaaat
      961 aaagataatt gtattcttcc cagaagatgg tgtaagccca gtacaaaaac ttcaaatgaa
     1021 aactcaaact ggaaaaaata catatgtagt tggtataaaa ggaaattttg atgatgcaca
     1081 atcaggtgta aagaagattt tttctgataa aaaatttaat caaaaactat tggataataa
     1141 ttatatatta tcttctgcaa attcaattaa tataggaaga cttcttccac aagttgcata
     1201 ctatttttat tcatatatga aattagttag caatagtgag ataaatttaa atgataaaat
     1261 aaattttgtt gtcccaactg gtaattttgg taatattcta gctggatatt atgccaaaca
     1321 aatgggtctt ccaataaaca aattgatatg tgcatcaaat gataataatg ttctttatga
     1381 tttctttaaa actggtgtat atgataaaaa tagaacattg aaattaacta cttctccttc
     1441 tatggatata ttaatttcaa gtaatttaga acgattactg tttgaaatat caaatagaaa
     1501 tactgatatt gtaaataagc tgttatcaga ccttagtgat aaaggagtct acaaaataag
     1561 tgatgatatg gaaaaaaatt tagcctcatt ctatggagaa tattcaaatg aagaagaagt
     1621 taaaagcacg ataaacaatg tgtttaaaaa ttataattat ttgatagata ctcatactgc
     1681 tgttgcatac aattgttatg aaaaatataa aaaagaaact tctgataaca caaaaactgt
     1741 tattgtaagt actgcaagtc cattcaagtt ttctgaagat gttctaaagt ctatagattg
     1801 tgattttaaa gaccttgatg actttgctat aattgataga ttatcaagta tttctaaaat
     1861 agatattcca aaacctatta aaaatcttaa aaatgcagaa atactacata aagatattta
     1921 tgaaaaagat gaattaaaac tagctataaa aaaattctta aaggtatagg tgattattat
     1981 gttagagatt atagttcctg caacaagtgc taatataggt ccaggatttg attgtcttgg
     2041 aattgcgtta aatatataca ataaatttta tgttgaagaa attgaaagtg gtcttgaaat
     2101 agaaggttgt gaagatgctt acaaaaacga aaataactta gtttatacat ctatgaagta
     2161 cttttttgat agagttaaac cagaaaaaat acctgctggt atcaaaataa aaatccacag
     2221 tgaagtacct atatgtagag gtcttggcag tagtgcctct tgtatagtag ctggagttat
     2281 agctgcaaat gctctatcag gtgctaattt agataaaaat caattattaa atatagcttc
     2341 agaaatagaa ggtcacccag ataatgttgc tcctgctatt ttaggaaata tgatcgtatc
     2401 tgttactgat aatgagaata tacattatga tattataaaa attcctgaag aacttaaatt
     2461 ttgtgcaatg atacctaatt ttaagttatc aacagaaaaa gctagaggtg ttttaccaaa
     2521 ggaaatacca tattctgatg gggtattcaa tgttagtaga gttgctttac taatatctgc
     2581 cttactaaac aaaaactttg acctacttaa agtagcttgt caagacaaat tgcatcaaaa
     2641 ttatagagga actctaattg agaactacaa tgatattgtt gaaaaatctg aacaattaaa
     2701 tagtattgga gtatttttaa gtggagctgg acctactata atgtctttaa taaaagaaaa
     2761 tgatgatagt tttgtggata atatgaaaaa ttacctacaa aaactaaaat ctgattggga
     2821 aataaaagag ttatgttgcg attctaatgg agctgtttta aatataatat aattatcgtc
     2881 ttctatacaa aatataacta ctcttttatt ataatttgta gtataataat taggactagg
     2941 taataatatt aagtattata atctagttct ttaaacatgt ttacattgag gagggattat
     3001 cataaaagag ttaataatta gagatttagt tgcaaaagtt cctataattc aaggtggaat
     3061 gggcgttggt atatctaggt catcactagc tggtgcagtt gctaagcttg gtggtgttgg
     3121 tgtaatttct ggagttcaaa ttggatatga tgaggaagat tttgaaacta atactataaa
     3181 tgcaaattta agagctataa aaaaacatat ttctaaggcc aaagaaatct caaatggtgg
     3241 tattattggt attaatttta tggtagctat gaaagaatat gaaacatacg taaaggaagc
     3301 tgttaaagct ggtgtagacc ttataatatc tggagctgga ttacctaata agctaccatc
     3361 attagtaaag ggaagtaatg taaaaatagc acccatagtt tcaactgcaa aagctgctaa
     3421 tgtaatttta aaaatgtggg atagaaaaga gaaaactact gctgacttaa tagttgttga
     3481 aggtccaaaa gctggtggtc accttggata ctctaacgag gaacttgata acatagattc
     3541 tatagactat gataaggaat ttgttgaaat tttaaaggtt gcaaatactt atggagaaaa
     3601 atttggtaga aacataccag tagttgctgc tggtggtata acttctagta gtgatgttaa
     3661 aaaatatata gatatgggtg caagtggtgt acaagttggt acaagatttg ttgctactta
     3721 cgaatgtgat gcccatgaga actttaaaat ggcatacata aatgctagtg aagaagatgt
     3781 tcatatagtt aaaagtcctg taggtcttcc tggacgcgcc ataagaaaca aatttataga
     3841 agaagttaaa ataaatcgtc cagaaataaa gaagtgctat aattgtttga ttccttgtaa
     3901 tccaaaagaa actccttact gtatttctca agctctaatc aatgcagtta aaggagatgt
     3961 tgaaaattca cttttatttt gtggaaatga tgcctataaa attgataaat taagtactgt
     4021 tgaagatgtt atcaatgaat tgatttctga attataattt aaagaaaact aaattaaaat
     4081 tttatcaagc taaggagttt taatacgtta tttattaatc tttataaaca tatttatgtc
     4141 cttagctttt ttgtgtcaat ttccttttat tgaaatttta atttaattag agatagtatt
     4201 aacccttata actaatcttc ttttacaagt acattggtta tttcagctat atataaagta
     4261 tgataatctt tttgtggata ccatcttcca tctatacctt cagcaataaa acattggggt
     4321 tcaagtttct gactataaat ttttttgcat ataatagcca ttttagcttc cttaaaataa
     4381 ggtgtatcat ttaagtgttc tatattaaat ccaatctgtg atatcttatc ctcttctcta
     4441 ccagatacct ttccacaata tgaaagctct ttacgaaaat cttcatcaaa aaatgtaaga
     4501 gaaaatttat ctgtattatc tataaattcc ttagtataac gctgtggtct tattactaca
     4561 aaagcaacat cttttcccca cataactcca agtcctcccc agctagcagt cattgtattc
     4621 actttacctt cattttctgc tgttataagt gtccaatctt tacctatcaa tccaaatgga
     4681 ttatttttaa tctcttctgc caaaatttct ttaaaatcac tcatatctct aataacctct
     4741 cattctctta tatttatatt ccttttattt tcgtgataaa ttataaatga agtctagttt
     4801 tgaaaataaa atactttttt aaatatatta ttgcaa
//
LOCUS       DAGQWR010000219         4801 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.222, whole genome shotgun sequence.
ACCESSION   DAGQWR010000219 DAGQWR010000000
VERSION     DAGQWR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.222"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            140..3238
                     /locus_tag="MIW95_003700"
     CDS             140..3238
                     /locus_tag="MIW95_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBY3152994.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGINDISHVK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKFKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFIVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMEVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFDDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDILTGLYN
                     YESFCEQTSLMLKRYPDKSYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDVHKLIYKIKNSLNRKKFEYMLVPCFGVYKILDNN
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLRHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            3361..4647
                     /locus_tag="MIW95_003701"
     CDS             3361..4647
                     /locus_tag="MIW95_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBY3152995.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAISENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
BASE COUNT         1939 a          442 c          862 g         1558 t
ORIGIN      
        1 ctatttacta aaaaatagaa ctttattaag gtactatttt aaaacataaa gaagtataat
       61 atcattgcca aatatatagc aaaattttaa aagctttaaa aattcaatct ggaaaatgaa
      121 taaaaggaga tatatataaa tggatgtaat aaatttaata gatgagttgg atgaaattgt
      181 atatgtttct tctttagata catatgaact tttatatatt aatgaaattg ggaaaaaggc
      241 aatgggaatt aatgatatat cccatgtaaa atgttataaa gcattacagg ggaaagattc
      301 tccctgcgaa ttttgtacaa actctatttt aaaagaagat gaatattaca tatgggaaaa
      361 tacgaatact cgaacaaata gacattttat attaaaggac aagttaatta aatgggaggg
      421 caaacatgca cgattagaaa tagctcttga tattacagaa aaggaagata taagtaagtc
      481 aatagcagaa aaattagaga taaaggagtt attagttgga tgtattaaac atcttataac
      541 agaatctaat ttttctgatg ctgtaaatct tgtattaacc aatattggta aatttcataa
      601 agctgataga gcatatgttt ttgaagtatc agaagataga aaaaaagtaa ctaatacata
      661 tgaatggtgc aatgaaggtg ttgatgaaca aaaacaggca ctaacaaatc ttgatattaa
      721 tgaattaaag aattggtttt tattattcga acaaaagggt tttgtaataa ttgatgatat
      781 agaaaatata aaagaagaac agccaataga atatgaaatt ttaaaacctc aaaaaattga
      841 aagtttaatg acatcttcac taaaacaaga tgataaagta gtagggttta ttggagttga
      901 taatccacat aaggctgttg gagatacctc tcttttgaca tccctatctt attttttatt
      961 aactgagatg aaaaaaagaa aagtagaaaa taaattaaat tatatgagct actatgactt
     1021 cttgactggt gcatataata gaaacaaata tattaagtat atagataatt ttcaaaaagg
     1081 aaaatttaaa tccataggag ttgtatttgt agatataaat gggttaaaag atattaatga
     1141 taactttgga catgctaatg gcgactctgc tattgtgtca gcatgtgtaa taataaaaaa
     1201 atactttcat tcttataatg tttaccgtat tggaggagat gagtttattg tattatgtga
     1261 agatatatca aaacaactat ttgaagaatt agtgagttgg ttaagtgaag aatttaagga
     1321 tgagatgata tatagtgtag cagtgggcag tatttggagt gacgataata ttgatataca
     1381 taagctaatt aaagttgctg atgagagaat gtaccaagat aagaagaggt attataataa
     1441 gaacaaactt agaaataatg aaatggaagt aattagtgac ttagctgatt cgacgaataa
     1501 tattttattt aaacataaag agttagttga taaggtttat tttgatgatt taatgataaa
     1561 agggtattct aaggcttatg aaaaattagc ttataataca tatgatataa ttttatctat
     1621 ttgcatcgat actaatttaa ttactccaat ttatcaatca gagagcaata tttttagttt
     1681 tcctataaag gaaaatcttg aaaaaacttt ttcatacata aaaaaacatt taattcatcc
     1741 tgatgatata atattatttg atgataacca ccttctaaaa tctcttgata gtcttaataa
     1801 cagcaagcaa gacgatttag aactaaattt tgagtataga cgtttaggaa tagataaaaa
     1861 gtactattgg gcattaattt atatgttaaa gattagtagt aaaaataagt taggtgagaa
     1921 acaaacacat attttagttg ctataaaaaa tatagattat cttatgcaac tacataaaaa
     1981 ccaaaaggat atattaacag gactatacaa ttatgaaagt ttttgtgaac aaacaagttt
     2041 aatgcttaaa agatatcctg acaagagcta tgcaattata atgatggata tagataagtt
     2101 caaggtaatt aatgacattt atggagttag aggaggagat aaggctttaa tatatgttgc
     2161 agaattgatt caaaaaaaca ttggttctaa tggtatatgt tgtcgtatgt atgcagatgt
     2221 attttgtatt ttttatgaga gttactctga tagagatgtt cataaattaa tttataagat
     2281 taaaaatagc ttaaatagaa aaaaatttga atatatgtta gtaccatgtt ttggggttta
     2341 taaaattttg gataataata ttccaattac aaatgcgtgt gaaatggcag gatatgctca
     2401 caaaaaagta aagaaacaaa gtatcaataa ctatatattt tatgatgata ctataagaaa
     2461 tgatgcactt gaagaaaaac agatggaaag ggaaatggaa tacgcacttg aaaatgggca
     2521 attccatgta tatctacaac ctaagtataa tcttaatgca aataaaattg ttggtgcgga
     2581 agcattggtt agatgggttc atccagaaaa aggtataata cctccaatta aatttatttc
     2641 actatttgag aagaatgggt ttattatcag attagacatg tatgtgtggg aacaagtatg
     2701 ttctttgatt agaaaatgga tagatttagg tgaagagcca gttcctatct ctgtaaatgt
     2761 atcaagatta catttgcata atccacattt taaagaaatt gttttaagtt taataaataa
     2821 atatagtata ccaaaatctt ttttagaatt ggagcttaca gaaagtttat ttattaaaaa
     2881 tataaaatta tttactaaga taatagccga ccttagaatg gatggtattg ttttaaatat
     2941 ggatgacttt ggttcagcat attcttcatt aaatatgtta aaaaatatta atattgatac
     3001 attgaagata gattgtggct tctttaatga ggaaggaatc acagacagag aaaaaatagt
     3061 tttaaggcac actatagcca tggcaaaaga tttagatatg gatgtagtgg cagaaggtgt
     3121 agagacaaaa gaacaagctg aatttttagt gagtttagat tgtgtggtaa ttcaaggtta
     3181 ttattattgt aagcctattc caagtgctga ttttgagaag ttactttatc aaaaataagc
     3241 taattatccc actctaaatg taggtaggat ggagtagttc aacataatta aatagaagta
     3301 gaaaatagtg aggatataat ttttaaaaag tataatattt atggattagg agcgaagttt
     3361 atgatagtgg gtattatagg tccttcagac tctgggttta agataaagga tgatttaaaa
     3421 cagatagaca gtgatttaaa gactaaaata tatgtaagag aaaaagtagt agatacaata
     3481 gaagttattt ctaaatgtga agatgaatgt gatgctatta tatttacagg atgtgcagtt
     3541 tatgaattta taaaatctaa gtatgagata agtaagccac attcatttgt accgagaagt
     3601 gggacgagta taatgaaggc attttgggca ataaaaagtg caaatataaa attagataaa
     3661 ttcagtatag atgtagttga tagatatgtt gtggaagatg cacttaaaga atttgatatt
     3721 aaagcaacga gtgtattttg taatccattt tctttggaag taggagagtc tgaacttgta
     3781 gaatggcata taaaattatt tgaagaaaat aagacggata taatgttaac tggttttggt
     3841 gcagtatata atgaattaaa agaaagaggc tatcctgtat ttcgtttaca agctactata
     3901 caattaatca aagaaagtta tgataaggta aagagtgaat atgctctaag caaggcaaga
     3961 ttttctcaaa ttgctgtcga aatattgagt ctaatagatt ataaagagaa gatagataac
     4021 tattattctg acatgataaa aaaatcagat atggataagc ttgtagtaaa ctatgtaaga
     4081 agcattcaag gttccttatt tttacttgga agaaatgatt atgttgtatt tgttcataaa
     4141 ggggtagctg ataatgagta taattatgat aagctattca atctaaagag agagataaaa
     4201 gacatgggct tttctcttag tataggaatt ggtactggag taactgcgta tcaggctgaa
     4261 aataatgcac ataaagcatt aagacactcc atagattcaa aagaaattgg tatatatttg
     4321 gtagatgaag atgaaaatat aagaggacct ttagcttccg aaaatgaatt aaattattct
     4381 ttgatgctat ctgatgaaaa ggtaatagaa atatctaaaa agactggaat gagttgtgag
     4441 tcagttgcaa aaattattgc tataagtgag aatagaaaaa gtaagattta tgattcaaaa
     4501 gagttagcag agtatctaaa tgtaagtgaa agaagtgcaa gacgtatatt aaataaaata
     4561 gtaaatgcag gtttaggtcg aatatgtgca aaagagacta gtattggtgg aggaagacca
     4621 aaaaatatta ctgaaatact gttttaagat attttataaa actgtataaa tcattatatt
     4681 ttataaaatt ctacaacaaa ttactatata aagtattaaa gcttatcatg aaaaaaatta
     4741 gaacaaatta ttatataaaa tactagagct tgttattaaa aatactagaa taaattatta
     4801 a
//
LOCUS       DAGQWR010000220         4794 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.223, whole genome shotgun sequence.
ACCESSION   DAGQWR010000220 DAGQWR010000000
VERSION     DAGQWR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.223"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            204..2075
                     /locus_tag="MIW95_003702"
     CDS             204..2075
                     /locus_tag="MIW95_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBY3152996.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTDTVTSKTIEIGIGDEKIDLTANPVDSTGTNLDPSTEGFRVNKIVK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISS
                     KDSETGKYKFTIKYTDASGKAIELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADVDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKADITDSQRNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            2182..2889
                     /locus_tag="MIW95_003703"
     CDS             2182..2889
                     /locus_tag="MIW95_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBY3152997.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELAINKIQYVHAP"
     gene            2922..4757
                     /locus_tag="MIW95_003704"
     CDS             2922..4757
                     /locus_tag="MIW95_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBY3152998.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWKKSENAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENISFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTRSKHDLIDSLAVGV
                     LASKNGSPILLAGNKLDSSQKDVLNTKIIDKVIQIGGLGNENVVEDVIDMQEETKYTV
                     ETIDELNVAIKKSDANDIIRFKPEKDKNITNSFKIETNKAITIEFDGIYKQSITIDMP
                     NGDIKNFGDIDNDLVINNIKNETLVNEGSIQGIDVYDENGCKIENEDSGEIWLITILK
                     NSKDVYILNRGEITKISNNSSSVTIKNSGNINTISGSEEPSISGKKPITNDTDKDEDK
                     KARGLYPKVESCIPALKDYVRITISTKPESSKYKIYYRVVDEKPSAKYVGEKISTKGW
                     SMVTISNQSFIERAKDGSYIEAVEIDTSNNEVSRWGKSSITDDGI"
BASE COUNT         2025 a          517 c          888 g         1364 t
ORIGIN      
        1 aaactggatt attaaaaaaa attacaattt tgttactttt ttgtatacat aaatattaaa
       61 aaaataaaat ataagaaaaa gtttatatct tttggttaat tattacaata agtctcattt
      121 attgaaataa tatcaaatat atattataat tggtaaataa ggaaaaaata ataaaaattt
      181 gaatttttta gggggaaaat accatgaata aaaaaaatct ttctgtaatt atggctgctg
      241 caatgataag tacatcagta gctccagttt ttgctgcaga aactacacag gtaaaaaaag
      301 aaacaataac taagaaagaa gctacagaac tagtttcgaa agttagagat ttaatgtctc
      361 aaaagtatac tggtggttct caagttggac aaccaatata tgaaataaaa gttggcgaga
      421 ctttatcaaa attaaaaata ataactaata tagatgaatt agagaaatta gtaaatgctt
      481 tgggagaaaa taaagaactt attgtaacta taacagataa agggcatata acaaatagtg
      541 caaatgaagt agttgcagaa gcaactgaaa aatatgaaaa ttcagcagac ctttccgctg
      601 aggctaattc tataacagaa aaagctaaaa ctgaaactaa tggaatttat aaagttgcag
      661 atgtaaaagc ttcatatgat agtgctaaag ataagttagt tataacttta agagataaaa
      721 cagacacagt aacttctaaa actatagaga taggtattgg tgatgaaaaa attgatttaa
      781 cagcaaatcc agttgattca acgggaacaa acttagaccc ttctacagaa ggatttagag
      841 taaataaaat cgttaaacta ggtgtagcag gagctaaaaa tattgatgat gtccaattag
      901 ctgaaataac tataaaaaat agtgacctaa atacagtttc accacaagat ttatatgatg
      961 gatatagatt aactgttaaa ggtaatatgg tagcaaatgg aacatcaaag tcaattagtg
     1021 atatttcatc aaaagattca gaaacaggaa agtataaatt tactattaag tatactgatg
     1081 catctggaaa agcaatagag cttactgtag aaagtactaa tgaaaaagat ttaaaagatg
     1141 ccaaagctgc attagaaggt aattcaaagg ttaaattgat agctggagat gatagatatg
     1201 caactgcagt ggctatagca aaacaaacaa aatatactga caatatagtt atagttaatt
     1261 caaataaact agttgatgga ttagcagcta caccacttgc tcaatctaaa aaagcaccta
     1321 tattattagc atccgataat gaaataccaa aagtaacttt agattatata aaagatataa
     1381 ttaagaaaag cccatcagct aaaatatata tagtaggtgg agaatcagca gtatcaaata
     1441 cagctaaaaa gcaattagaa tcagtaacta agaatgttga aagactagct ggagatgata
     1501 gacatatgac ttctgtagca gtagcaaaag ctatggggtc ttttaaagat gcatttgtag
     1561 taggtgcgaa aggggaggct gatgctatga gtatagctgc caaagctgct gaacttaagg
     1621 ctcctataat agtaaatggc tggaatgatc tttcagcaga cgctatcaaa ttgatggatg
     1681 gaaaagagat tggtatagtt ggtggttcta acaatgtatc tagtcaaatt gaaaatcaac
     1741 ttgctgatgt tgataaagat agaaaagttc aaagagttga aggagaaaca agacacgata
     1801 ctaatgctaa ggttatagaa acatattatg gaaaattaga taaactatat atagcaaaag
     1861 atggatatgg aaataatggt atgctagtag atgcattggc agcaggacct ctagcagcag
     1921 gtaaaggtcc aatacttcta gctaaagctg atataacaga ctcacaaagg aatgcactta
     1981 gtaaaaaatt aaatcttggt gcagaagtaa ctcaaatagg taatggagtt gaattgacag
     2041 taatacaaaa gatagctaaa atactaggtt ggtaataact tatactgtgg agaagattcc
     2101 ttaattaata caggaatctt tttctataat tatgctattt attgtaaatt ataacatttt
     2161 ataaagtagg aggatgtagt agtggtagat gcaactttat tttttacacc acatcaagat
     2221 gatgaaacct taagtatggg aagtgctata attgaacatg ttgaaaaaag tgatacacat
     2281 gtcatattat gcacagatgg tagtaaatct ataataagaa aagttttgga tgatggaggg
     2341 tcatgttctt atcatattaa agatgttcat aaatactctt tatcagaaag tgaattttca
     2401 aaagatagag atgaagaatt taaggatagc tgtgaagcaa tgggtgtaaa agaaagtaat
     2461 atacatatag aagataatag agcacatgat ggtgagttaa gtaaagaaaa ggcaagggaa
     2521 ataatactta agtacttaga agaatatcca gatgcaaaag taaaaactgt tactccgttt
     2581 aaagctagtg gaatacatga agaccatagg gcattaggag aagctgcact agagttatat
     2641 aaagaaggca aaataaaaga tttaagattt tatgtagaac catacgacta taaggatttt
     2701 aaaaaagtta atccaaatgt agaggtatgg aaggtattac ctagtcaaga ggagaagtta
     2761 ttaagtgcaa tgaatgcata taaaaaatgg aatcctgaaa gtggacatta tgctatagga
     2821 tatcattcag taaaatctca ttttgatgag ctagctataa ataaaataca gtatgtacat
     2881 gctccataaa atattatgaa agaattagga ggtaagaatt aatgaaaata tcaaaaaaga
     2941 tagtgtcttt gttaactatg acatttttaa ctgttacatt atatggaaat acatctaatg
     3001 catctacaaa agatacatta acaggttctg gaagatggga aacagcaata aaaataagtc
     3061 aagcaggatg gaaaaagtct gaaaacgctg tgctagtaaa tgataattca atagcagatg
     3121 ctttatcagc tactccattt gctaaagcaa aagatgcacc aatattatta actcaaagta
     3181 ataaattgga tagtagaaca aaagcagaat taaaaagact tggtgtgaaa aatgtatatt
     3241 taataggtgg ttcaattgca ttaagttcag agattgaaaa gcagttaaat gcagaaaata
     3301 taagttttga aagaatatct ggaaatagta gatatgatac ttctttaaaa ttagctgaaa
     3361 agctagatag ggaaaaatct atatctaaaa tagtagtagt aaatggagaa aagggtcttg
     3421 ctgatgcagt gagtgttgga gctatagctg ctcaagaaaa catgccaata atactttctg
     3481 attcagagaa tggaactgaa gtagctgata attttataga tagtaaagat atagaaaaat
     3541 cgtatgtaat aggtggtaca tattctattt ctaattctgt agaaagaagt ttaccaaatg
     3601 caacaagaat agcaggtagt agtagaagtg agacaaatgc aaaaattata gaagaatttt
     3661 acaaagatac tgatataaaa aatatttatg taacaaagga tggtacaagg agtaagcatg
     3721 atttaataga ttctttagct gtgggtgtat tagcttctaa aaatggttct ccaatacttt
     3781 tagcaggaaa taagctagat agttctcaaa aagatgtgtt aaacactaaa attatagata
     3841 aagttattca aattggcgga ttgggtaatg aaaatgttgt agaagatgta atagatatgc
     3901 aagaagaaac taagtatact gttgaaacta ttgatgaact taatgttgct ataaaaaaat
     3961 cagatgcaaa tgacatcata agatttaagc cagaaaaaga taaaaatata actaattctt
     4021 ttaaaataga aacaaataaa gctataacta ttgaatttga tggtatatat aaacaatcaa
     4081 taactataga tatgccaaat ggagatataa aaaactttgg ggacatagat aatgatctag
     4141 taataaataa tataaaaaat gagactttag ttaacgaagg aagtatacaa gggatagatg
     4201 tttatgatga gaatggatgt aaaatagaga atgaggattc tggagaaata tggcttataa
     4261 cgatacttaa aaattcaaaa gatgtatata ttttgaatcg tggagaaata actaaaatat
     4321 ctaataattc ttctagtgta acaataaaaa attcaggtaa tattaatact ataagtggaa
     4381 gtgaagaacc atcaataagt ggtaaaaaac ctataactaa tgatacagat aaagatgaag
     4441 ataaaaaggc acgtggttta tatcctaaag tagaatcttg tatacctgct ttaaaagatt
     4501 atgtaagaat aactatatca acaaaaccag agagttcaaa gtataaaatt tattatagag
     4561 tagttgatga aaaaccatct gcgaaatatg ttggagaaaa aattagtact aaaggttgga
     4621 gtatggttac aatatcaaat caatctttta ttgaaagagc caaagatgga tcatatatag
     4681 aagcagtaga aatagatacc tcaaataatg aagtttcacg atggggaaaa tctagcatta
     4741 cagatgatgg aatttagaca ttaaacttat tcattttgca gattactatt tagt
//
LOCUS       DAGQWR010000221         4762 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.224, whole genome shotgun sequence.
ACCESSION   DAGQWR010000221 DAGQWR010000000
VERSION     DAGQWR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.224"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(198..1001)
                     /gene="proC"
                     /locus_tag="MIW95_003705"
     CDS             complement(198..1001)
                     /gene="proC"
                     /locus_tag="MIW95_003705"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBY3152999.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGINTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            complement(1326..3695)
                     /locus_tag="MIW95_003706"
     CDS             complement(1326..3695)
                     /locus_tag="MIW95_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBY3153000.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRLTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPKDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            complement(3700..4608)
                     /locus_tag="MIW95_003707"
     CDS             complement(3700..4608)
                     /locus_tag="MIW95_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBY3153001.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGVNIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
BASE COUNT         1410 a          912 c          515 g         1925 t
ORIGIN      
        1 tgatatatct caaatatagt taaaaatata tctatttaat tattaatatt taagtaataa
       61 attctcactc aatcattaaa ttccttttta tagtaaatta ctcttatttt acttatacca
      121 aatacattta tgtatagata taaataagta ctaaaaaaac aattcaagaa accatatagt
      181 aaacttaatt acatctgtta tttgctcata tctttagact tatctataca agcaacaata
      241 gattttataa ctgctgctct aaagccttct tcttctaata ccttaacagc ttctatagta
      301 gttcctgctg gagaacaaac catatctttt aattcacctg gatgttttcc agtttcaaga
      361 accatcttag ctgaccccat tactgcttga gatgcaaatt tataagcttg ttgtcttggc
      421 attccagcta atactgctgc atctgccata gcttctataa acataaatac atatgcagga
      481 gaagaaccac taatacctgt aactgtatcc attaaatatt ctgatactat ttctgcctca
      541 ccaaaagatt caaacagagc cttaatcata tttaactctt catctgttac attactattt
      601 ttacaaagag ctgacattcc ctcgccaaca agggctggag tattaggcat tactcttact
      661 atttttttat ctgaacctat aaaactttcc agactttcta tagttttacc tgctgctata
      721 gatactatta ttttatttgc atctattaat tctttaagtg gagttaaagc cttttcgcat
      781 atattaggtt taagtgctac tattaccaca tcagaatttg taacagctac ctttgaatca
      841 gttgtagtat ttattccaaa ttcttctttt actttatcta aggcaacttg gcttaaatct
      901 gatacagtta tatcttctgc ctttactaat cctgactgta ctatgccacc tatcattgca
      961 cttcccatat tccctgaacc aataaatcct aaagttttca tatcaaatct ccttttttct
     1021 attagtttta ttttaaagta gctaaattga ctttatttta actgcttaaa attatcttat
     1081 ttttataaaa tataacctta ttataactat aataaattat tcttttgtat gtattactta
     1141 ctaatatgtg tatgatttac taaatataaa tttaacgatt ttttcattaa acttaacgag
     1201 ctaacttcct atttagaaag ttagctcata attaatctaa aattttaaaa atggaatcaa
     1261 accatttata ctttattata tcagttacga ttttatatag taagaagttt tatttacaag
     1321 caaacctaga atgtttgctc agttcttcct attatctcat cttgtagtgt tctacttaaa
     1381 ttattgaaat ggtcactata tcctgcaact ctaactatta agtctttata atccttagga
     1441 ttcttttgag cctctaacag tacattttta tcaaatacat taaattgtat atgatgacca
     1501 tccatattaa agtatgctct tactagatta gccatattat ctaacccttt ttctccttga
     1561 actactgatg gtgcaaatct ttgatttaat aatgttccac cagtttttat gtggtccatc
     1621 ttagcacaag attttataac tgctgtaggt ccattagtat ctccaccttt ttcaggtgaa
     1681 attccctctg aaactggttt tgcacaaagt ctaccatttg gacttgctcc cataatttct
     1741 ccaaagtata tatgacaagt tgttggtaac atatctactc tgtattctcc accacaaaca
     1801 gttagtctac cagttacctc attgtaatat gcattgaata tctcttgcat tatttcatca
     1861 gcataatcat catcatttcc atatttagga gtcttatttg aaactaaatt aagtatagct
     1921 tcatatcctt caaaattagc atcaagtgct ttaagcaatg tatccatatc gaattttttc
     1981 ttatcaaata cattatattt tattgctgtt aaactatctg ttatagttcc tataccaaca
     2041 ccttgtatat atctagtgtt gtatcttgct ccacctcttt ggtaatcctt gcctttttct
     2101 atacaatcat caactattgt agacataagt ggacaaggca tgtgttttgc acatatattt
     2161 tctatcacag catttccttc tatttttatg tcaatcatat aatgtaattg ctttttaaat
     2221 gcttcaaata gttcctcata agattggaaa tttctagggt ctccagtttc aatacctatt
     2281 tgttttttac taattgggtc atatccatta tttaatgtta attcaagtat ttttggaatg
     2341 ttcatatatc cagttagaac atatgcttct ttaccaaaac atccagtttc aacacaacca
     2401 ctggttccac caagtcttgc atcttctata gtttttccag cttccataag ctcttgaact
     2461 attgcttcag tattataaaa tgctggttga ccccatcctt ttcttgatat ttcacaagct
     2521 ctctttaaga atttttgagg agtcttttta cttatttgaa catttgaact aggttgtatc
     2581 aatttcattt catccataac atcaagtatt atataactaa cttcattaac accatcttga
     2641 ccatctggat ttattccacc agtgtttata tttgcaaaat ctgtatatgt gctactttct
     2701 tttaatgtta taccaacttt tggtggtgct ggctgattgt taaatttaac ccataaacat
     2761 tcaagtaatt cttgtgctct atctctatct agtattccat tttctacatc tctttcatag
     2821 aaaggattta gatgttggtc aagtcttcct ggagtaaaag catcccatat attaagttca
     2881 gtagtaaccc ctatatgaac aaaccaatac atttgtatag cttgatgata agtttctggt
     2941 ttgtgagctg gtacaacatc acaagtttca gcaattagta ataaatcttt tttcttagct
     3001 tcatctgttt cttcttctgc catttgtctt gctttctcag catatctttt acctaatata
     3061 accattgcat cacaagctat tgccatagct tctaaatctg cttttttatt atatgcatct
     3121 aaatcattaa gaaaatctag ctcttttaat cttgcttcta tgtctttctt taaatctaaa
     3181 aatccttttt tatatattgt atctccacaa actgtatgtc ctggggctct ttgttccata
     3241 aactctgtaa acatacctgc ttcatatgct gctaaccact caggtgtcat tgcatttatt
     3301 atcttatctc ttgtttgtct ttttttccaa aaaggaatta tttcttcttt atgtattttt
     3361 ctagcttctt ctgatacgct aaaatttatt atatctctat tgtgcattac ttctaaatct
     3421 tccattgtgt gacaacaaat ttctggatat gttggtgctc cattaggaga atcacctttt
     3481 tcacctacta ttaattctcc atcatttatg ctaagagttc tattctcaat ataatgctta
     3541 aatgataaag cacgaagtac aggaatctct acacttccct catatttttt ataagcttct
     3601 gtcataagta ctgctctttc aatagatatg tgtggttctg cattaatact ttcttctctt
     3661 aattttttag ttctctcaaa agttcctctt gccattttat taccctccaa tcttagtatt
     3721 gaaattactt gcttcaaaca gtctttttat ttcctctaat ttttcttctg atggtctttg
     3781 tagttcttct ccttcatact ttttataaat tttatcatac ttgtgtttac caatattatg
     3841 atatggaaga agacttactg cttgtatatt caaaggcttt aaaaactcta tcattttttt
     3901 aacctctaaa ttttcatcat ctacatttac tcctacaatt aatggtattc ttatatttat
     3961 gttaacccct aattcactta aaatcttaac atttttcagt attaagtcat ttgattttcc
     4021 tgtaaatttt atgtgcttat cctcatcaat aagcttaata tcatataaaa acaaatctgc
     4081 acatttagca actctttcat aattttctga ctttgcatag ccacatgtat caattgctac
     4141 atggattccc tttgatttgc acatgtttat tactccacaa atgaagtcca tatcttgagc
     4201 cattacttct ccacctgata gtgttactcc accacccgat tcttcataga aaattctatc
     4261 cttctctatc tctttaacca agtctcttac agaatactct tgaccaacaa tctctcttgc
     4321 attgtttaca caatagtcta agcaagtttc acaaaactca cacttatctt ggtctaagca
     4381 tatttttgtt tcacctttgt aaattgcctt atgagggcat atatttatac atgcttcaca
     4441 ttttgaacat ctttcttcat tatacaaaac ttgttttgta tatgtttgac tctctggatt
     4501 atgacaccaa acacattcaa gtggacaccc tttaaaaaat actgtacttc ttatccctgg
     4561 tccatcatgt atgctacatt tttgtaagtt tataactaat ggattcatga tttttcaccc
     4621 ctaaataata tccattccat tttttcattt ttatgcaatt catcttcata gaatatacct
     4681 atgaaaagca tttctacaat tacataatac tatatttgtt attattttgt ctatatattt
     4741 ttagtaatat taaaaaattt ta
//
LOCUS       DAGQWR010000222         4761 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.225, whole genome shotgun sequence.
ACCESSION   DAGQWR010000222 DAGQWR010000000
VERSION     DAGQWR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.225"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            80..741
                     /locus_tag="MIW95_003708"
                     /pseudo
     CDS             80..741
                     /locus_tag="MIW95_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(960..1997)
                     /locus_tag="MIW95_003709"
     CDS             complement(960..1997)
                     /locus_tag="MIW95_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBY3153002.1"
                     /translation="MNKKLIKAKIFIWSILAISLSIVFISLLIGTIISHSFNFPFNTL
                     NPKYNYDNYIVAKNESFSDKINDINVDWISGEVRIKRSDSNKIKLIQKIPSNFSKKKL
                     AKIMVDGNSLSIYDNSTKKFFIGINLPKPTILELYLPNKLYDKITLYTTSADIYSTYI
                     RSNTVEANTISGAIGLSGNINKINLDTTSGHIDITKLNSKNATLKSTSGEIQLSGKID
                     VLNLETTNGNINLKDIYNKNLTCSSVSGKMNLSGVFSDIKAESTSSNIKINSSKMLSS
                     LYCETTSANVDLSIPDNPGFTLNFDKVSGVLDSDFELNRHSDLYTYKNGITNLYLSTT
                     NGNLTIIKNNH"
     gene            complement(1990..2676)
                     /locus_tag="MIW95_003710"
     CDS             complement(1990..2676)
                     /locus_tag="MIW95_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153003.1"
                     /translation="MLDKIRTYIDSSFTGVNETKKVKELKDELFENLKEKYNDQIQDG
                     KTEQEAYNSVISGIGDLSELIESVKEPYSLPSELIEEKKKRALRASIAVALFIISPFL
                     FVVSVVSFGIEPTIAILLMLILIALGVGLLVYNQMTRTNYALYNDNLSNKFKEFEDFE
                     ENYNHINPVYKSFMNAYWIIVVSIYLITSILFNIWSFSWILFIIGAAGVNIVKGIIQL
                     KGYKEEDKYE"
     gene            complement(2688..3008)
                     /locus_tag="MIW95_003711"
     CDS             complement(2688..3008)
                     /locus_tag="MIW95_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3153004.1"
                     /translation="MIIASDMIRGHTETIILKHLLEKDSYGYEINKSILEKTKGLYEL
                     KEATLYTAFRRLEQSGAITSYWGDEVTGARRKYYSITNIGKEKYIKSREEWNNTKALI
                     DILI"
     gene            3369..4709
                     /locus_tag="MIW95_003712"
     CDS             3369..4709
                     /locus_tag="MIW95_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBY3153005.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLFLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         1754 a          523 c          706 g         1778 t
ORIGIN      
        1 ggagaattag taagacatat gttagtatgc aaaattctat gaagcaggaa ccctgtgact
       61 ttagtcatgg gaggttcaga ttttggctga agatgaagaa aataatcata taaatattga
      121 gatgcaaaga gcttttgaga acgattattt ggaaagagct gaatattatc ttagtcgtgt
      181 acatggtaga aagttagaag aaggtaaaga gtataaagaa attggaaaaa ctattggaat
      241 acatatacta aatcatgtaa aatataatca catagaagat tatgtaaatt gcctgagatt
      301 aacaatggac gagcatccag atatttattc tagtaaaact gcattatatt ttatagagct
      361 tccaaaaatt cataaatctg actatatagt aaatagaatt atgctatggg gaaaacttat
      421 tgataatcct tctagctcag atgtaagagt tatagctaag aatgattcta ttattaaaaa
      481 agcacttgat agattaaaag aattaggtag caatgaagaa tatttattaa atctgaaaag
      541 aggtgcttat atcatgaata aaagtagaaa ttttaaagaa gagattgaaa gagagacagc
      601 aattagagtt gcaaaagaaa aagattataa aataattata atgttgaaaa aaatggtttt
      661 aaattagatg acatattgaa taaatttaat gattttgact taagtgaaga tgagataaaa
      721 gaagtatatg aaaataattg atgataaata aaaagtgaac ttatataagt ttgctgaatc
      781 tgagcctgga aatatttatg tgtatttttt aatgaataaa aatttatttt aaaaacaata
      841 attagaggta cagttaatta tatttttatg atactgtact tctagttttt tctattgtag
      901 atttattatt tactaataaa tctacaatag aagtgctttt gttgtaagaa tcattttatc
      961 taatgattgt tttttataat agttaaattt ccatttgtag tgcttaaata taaatttgta
     1021 ataccatttt tataagtgta taaatcacta tgacgattca actcaaagtc actgtccaaa
     1081 acaccagaaa ccttatcaaa atttaaagtg aaacctggat tatcaggaat agacaaatcg
     1141 acattggcag aagtagtttc acagtacaga gaagacaaca ttttgctaga attgattttt
     1201 atattactgc tagttgactc agctttaatg tcagaaaaaa ctccagataa gttcattttt
     1261 ccagaaactg aactacaagt aagattttta ttgtatatat cttttaaatt tatattgcca
     1321 ttagttgttt ctaagtttaa aacatcaatt ttcccagaaa gctgtatttc accagatgtt
     1381 gatttgagtg tagcattttt actatttagt tttgttatat caatgtgacc actagttgtg
     1441 tctaaattta ttttattaat attaccagag agacctattg cacctgaaat tgtgttagct
     1501 tcaactgtat tacttcttat gtaagtgcta tatatatctg ctgaagtagt atataaggtt
     1561 attttatcat aaagcttatt gggcaaatat agttcaagta ttgttggttt tggaagatta
     1621 attccaatga agaatttttt agttgaatta tcatatatag aaagagaatt tccgtcaacc
     1681 attatttttg ctagtttttt ctttgagaaa ttactaggga ttttttgaat taatttgatt
     1741 ttattagaat cacttctctt aattctaact tcacctgaaa tccaatcaac attaatgtca
     1801 tttattttat ctgaaaaact ttcattttta gccactatat agttatcata attgtattta
     1861 ggattaagag tattaaaagg gaaattaaaa gaatgtgaaa ttatagttcc tataaggagt
     1921 gagataaata caatagaaag tgatattgcc aggatactcc agatgaaaat ctttgccttt
     1981 ataagctttt tattcatatt tatcctcctc cttgtagcct tttagttgta taattccttt
     2041 gacaatgttg acaccagcag cacctattat aaatagtatc cacgaaaagg accaaatatt
     2101 aaatagtatg cttgtgatta gatatataga aactactata atccaatatg cattcataaa
     2161 tgatttataa actggattta tatgattata attttcctca aaatcctcaa attctttgaa
     2221 tttatttgat aaattatcat tgtatagagc atagtttgtc cttgtcattt gattatagac
     2281 taataaacct actccaagtg ctattaatat gagcattaaa agaatagcta tagttggttc
     2341 tatgccaaaa cttacaactg agactacaaa taggaaaggg cttattataa aaagagcaac
     2401 tgctattgat gctcttaagg ctcttttctt tttttcttca attaattcag aaggtagaga
     2461 atatggttcc ttaacacttt caattaactc agataaatca ccaattcctg agattactga
     2521 gttatatgct tcttgttctg ttttaccatc ttgtatttgg tcgttatact tttcttttag
     2581 attttcaaat aattcatctt taagttcttt gacttttttt gtctcattaa ctcctgtaaa
     2641 gctactgtca atataagtac gtattttgtc taacatattt tcacctccta aattaatata
     2701 tctatcaatg cttttgtatt attccattct tcacggcttt ttatatattt ctctttacct
     2761 atgtttgtta ttgaataata ctttcttcta gctccagtaa cttcatcacc ccaataagag
     2821 gttattgcac cagattgttc aagtctacga aatgctgtat acaaagtagc ttctttcaac
     2881 tcatacaatc ctttggtttt ttcaagtata gatttattaa tctcataacc ataactgtct
     2941 ttttcaagta aatgttttaa gataattgtt tctgtatgac ctcttatcat gtcagatgcg
     3001 attatcatga aaatcacctc caattaaata tataatactt tgatatactt cgcctgtcaa
     3061 ggtatatcat gagaaaagac attttattgt aatatttata aggttttata tagaaataat
     3121 gtaaaaactt tataaataat atattttata cctagttata gataacttta tatagttatt
     3181 attaaaaaaa gcaataaaaa gtttaattca atttagcaaa tttagtgata taattaaaaa
     3241 agaaaagaaa tatggactta tttggaataa ttttgacaat ctatttacta cacgtgtaga
     3301 gtcgtatata attaaattat acataaaata taaaaaaata atttagtaat acgttaggag
     3361 aaagtattat gataaagtca agcttatttt ggaatatttt cattgcgata tcctttatga
     3421 tagaatggtt tctttgtaag aaaatgctag attatacaag tgagaaaagg atttctaaca
     3481 aaaaaataaa cttttgtatg gtaggtatta ttgtttttgc tgacataata tatttttttg
     3541 atgtacctcc aaatataaga gtaattatat ctatgataat tactattgca ttttataaaa
     3601 tctcatatga tgtgggattt ataaagtctg tattaatatc cttaatttat tggatgttac
     3661 taatcgccac agatgctttg agtatatctt taacagtttg gcttaattct ataaatgaca
     3721 tgagtagtat ggggacggga gatatataca gaattgagtc tataatattg ggaaagatac
     3781 ttttgattgt gtctttgttt ttatatagag ccttaaagat aaaacttaat atagaaataa
     3841 ataaaaggac attattttat atagttattc ctatagtcgc taatatagca tctttttttg
     3901 taatttttga atacatattt aatccagggt ctggtagctt aatacataat ttccaattcg
     3961 ttatgatttc tgttgtattg cttatatcaa gtatcacttt agttttaatt gctaaaaaat
     4021 tacagcaata taatacttta gttgtagaaa acaatatgat taaaaatact ttgaagtatg
     4081 aaaatgagta ttatgaagat atagaaaaga aatatttaaa aactagatta ctaagtcatg
     4141 atatgaaaaa tcacatactg tgtataaatg ctatgattaa aaaaggtatt gatgttactt
     4201 cttatttaaa taatttgcag gtagaaattc agagtaatga cttgttattt aatactggta
     4261 attttatatt agatgctata ttaagtgaaa agaaagaagt ttgtgataag aatgatatag
     4321 aactaaaaat aggaataaat tttagttgtt gtgaagctgt tgaattagta gatatatgtc
     4381 atatattttc aaatatttta gacaatgcaa ttgaggcatg taataaaatt gatagtggaa
     4441 atagagttat ttgtgttaaa ggtgatgtta tgcaaaattt ttatttaata agagttgaaa
     4501 atactaagat gaataagatt attactaaaa atggagatat tttgacagac aaaaaaggtt
     4561 ctcttcatgg acttggaata aaaagtgtaa aaaactctgt aaataagtat ggtggtaatg
     4621 ttgtaattga atatgatgat aaaaaatttg tagttaaaat tgctattcca cttagaaatg
     4681 tgaagaaagc agttaatcat gtgatttaaa tgtatataaa tttgatgtgt agaaatttaa
     4741 tatataagaa ttaatgtata a
//
LOCUS       DAGQWR010000223         4749 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.226, whole genome shotgun sequence.
ACCESSION   DAGQWR010000223 DAGQWR010000000
VERSION     DAGQWR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.226"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            328..939
                     /locus_tag="MIW95_003713"
     CDS             328..939
                     /locus_tag="MIW95_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153006.1"
                     /translation="MNIKRINLLLSTVMAAVIISLPLAVTANAMEKTESDNNVKIELS
                     DKEVEELHNYWPEDKIFSDEKIDEILIKTGQFTQKELDKINQEVDDSAKKVNQNSRVK
                     YINGYNKYRTFTKNGKNYLYIYVSGKTLKKIKAGTDLASTFGGFLPKIWVELDVVAIG
                     KIITINMDGINTNYGIVLSYIEDKWNFQGATYTYRYDHWFYQT"
     gene            967..1149
                     /locus_tag="MIW95_003714"
     CDS             967..1149
                     /locus_tag="MIW95_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153007.1"
                     /translation="MKKRLYKVFLKTIPNLIIVILSLALSFYTNTFDIDLKILLLIPL
                     AYVLLFIYYYIKTKKD"
     gene            1389..1931
                     /locus_tag="MIW95_003715"
     CDS             1389..1931
                     /locus_tag="MIW95_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY3153008.1"
                     /translation="MLIYLAILELEEDKIKFEKVYEKYRQIMFYVANKILKDDHLSED
                     AVHNAFLRIIKNIDKIDEVDSPRTKAFIVIIVERIAIDFYRKRKREKVSDIEEEYKNR
                     EINFSIEDKVCESNLAIALAKLNESYFQVLSLKFQYGFSNKEIANALNLGEENVYKRI
                     QRARKKLKEILEEMEVGNIE"
     gene            1963..2625
                     /locus_tag="MIW95_003716"
     CDS             1963..2625
                     /locus_tag="MIW95_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HBY3153009.1"
                     /translation="MLRESIPIATKIIIDDLLSDDELNHVFSKGFERKMKKLIKQQKR
                     SSLTNKIIFYSKQTAIVFLIVLASLLTITMSSEALRSRFFRLVIRVYEDLTSFVFSTN
                     GEDNLSLKNKEPEYIPKGFKEVDRVDDFVITYRNDENIEIQYVTDKISSNSIILDTEN
                     AKVKDIFVNGHKAKYIVKGTFLQLFWNDNNFIYLLNVDYQNSGESEFYEDILIKIAKS
                     IK"
     gene            2815..3132
                     /locus_tag="MIW95_003717"
     CDS             2815..3132
                     /locus_tag="MIW95_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153010.1"
                     /translation="MKSDLYLCKSNKLYRDNEVLEKFMEDLSFLIDDYKFSLETISIL
                     CKINIEKLTNFYAGKGYILYEEMSLIESVLAPFLSAIDIAKHNYDVVIKSRESDKPLI
                     FEN"
     gene            3154..3597
                     /locus_tag="MIW95_003718"
     CDS             3154..3597
                     /locus_tag="MIW95_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153011.1"
                     /translation="MKRLFALLLTLSLVILPSTLVFANEKAENDSIYAEAPLVFQSNE
                     KAYTHGSGGTAYIGYMSSKHLNWRITSSKGIVILFSGTVEIRNSKNMLVASYPISGGG
                     SSTISGQIDVKYLKKGDYKAILKGTSTTGGGVLVVKSGLVQMFTI"
     gene            3815..4258
                     /locus_tag="MIW95_003719"
     CDS             3815..4258
                     /locus_tag="MIW95_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153012.1"
                     /translation="MNLRKTVISGMCALVCSSAVAIPAFADEYQDKNIEQYSAVNYID
                     DTMVKMSYIKSGTCNLSISGSTAKVTCFVRGASNVTSTSIIARLQKSNDNGKTWSTIQ
                     TWNASGNISCSLSKSKTIGKGYYRVHSTIKANSESKNIISSTKKY"
     gene            complement(4389..4749)
                     /locus_tag="MIW95_003720"
                     /pseudo
     CDS             complement(4389..4749)
                     /locus_tag="MIW95_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT         1903 a          500 c          724 g         1622 t
ORIGIN      
        1 ttcaaagaat acacataaat atttccagac tcacttattg tataagttat attctatttt
       61 gtatgaagtc aattaaaaac tgaactatat taaaattaat tttataaata acaaatataa
      121 ataattacaa aaccttaact gcgttaagtt tgaaatataa taatattaaa aacacttctc
      181 tcaaatcaag tatcttttgt tatattttca taagttttaa aaatataaat tttttaaaac
      241 ttgtgtccaa aaaacaaaat taaatatgtt atatatgtaa agggttaatt ttaaaacctt
      301 aaatttatta tagttagggg gatttaaatg aatatcaaaa gaattaatct ccttttatca
      361 acagtgatgg cagctgtgat aatatcttta ccacttgctg ttacagcaaa tgcaatggaa
      421 aaaacagaat cagataataa tgtgaaaata gagttgtctg acaaagaagt agaggaatta
      481 cataattact ggcctgagga taaaattttt tcagatgaaa agattgatga gatcttaatt
      541 aaaacaggtc aatttactca aaaggaattg gataaaatca atcaagaagt ggacgattct
      601 gctaagaaag taaatcaaaa ttctcgtgtg aaatatataa atgggtataa caaatacaga
      661 acttttacta aaaatggtaa aaactattta tatatttatg taagtggaaa gacattaaag
      721 aaaataaaag ctggaacaga tcttgcatct acttttggag gttttttacc aaaaatttgg
      781 gtagagcttg atgtagtggc aattggaaag ataataacaa ttaacatgga tggtataaat
      841 actaattatg ggattgtttt atcatatata gaggataagt ggaattttca gggtgcaaca
      901 tatacttata gatatgatca ttggttctat caaacttaaa agacatgctt ttttaggagg
      961 aataatatga aaaaaaggct ttataaggtt tttttaaaaa caattccaaa cctgataatt
     1021 gtgatattat cacttgcatt atcattttat actaatacat ttgatattga tttaaaaata
     1081 ttgcttttaa tacctttagc atatgtactt ttgtttatat actattacat caaaacaaaa
     1141 aaagattaat ttttatggaa ttagatatat acgaaaataa ttaatttaaa aatttaactt
     1201 tgagaatcat ttgtagagtt aaggccctta taatcccgaa aatggatttg aagggccttt
     1261 ttactagata tctttaaaaa gcttgttaag catagttata ttctatttaa gtagagttaa
     1321 ttatatattt atgttataat tgaactttaa aatattaatt cagaattttt atattggagg
     1381 aattctaaat gttaatttat ctagctatac ttgaactcga agaagataaa ataaagtttg
     1441 aaaaggtata tgaaaagtat agacaaataa tgttttatgt agccaataaa atattaaagg
     1501 atgatcattt atcagaagat gcagtacata atgctttttt aagaataatt aaaaatatag
     1561 ataaaattga tgaggtagac agtccaagaa cgaaggcttt tatcgttata atagtagaaa
     1621 ggattgctat agacttttac agaaagagaa aaagagaaaa ggtatctgat attgaagaag
     1681 agtataaaaa cagggagata aatttttcta tagaagataa agtatgtgaa agtaatttag
     1741 ctatagcact tgcaaaactt aatgaaagtt actttcaagt gctttcctta aagttccagt
     1801 atggtttttc aaataaagaa atagcaaatg cattaaattt aggggaggaa aatgtatata
     1861 agagaattca gcgtgctaga aaaaaattaa aagaaatatt agaagaaatg gaggttggaa
     1921 atattgagta ataaaaaaga tgattttgat attacggatg atatgcttag agaaagtatc
     1981 ccaatagcga caaaaattat tattgatgac cttctttcag atgatgaatt aaatcatgtt
     2041 ttttcaaaag gttttgagcg aaaaatgaaa aaactcatta agcaacaaaa aagaagtagt
     2101 cttacaaata aaatcatttt ttattcaaaa caaacagcta ttgtattttt aatagtttta
     2161 gctagtttac ttactattac tatgagtagt gaggcattgc gttcacgttt ctttagatta
     2221 gtaataagag tatatgaaga cttaacttca tttgtttttt caactaatgg agaagataat
     2281 cttagtttaa aaaataaaga acctgagtat ataccaaaag gatttaaaga agttgataga
     2341 gttgatgatt ttgttattac atatagaaat gatgaaaata ttgagataca atatgtaaca
     2401 gataaaatta gttcaaattc tattatatta gatacagaaa atgcaaaagt taaagatata
     2461 tttgtaaatg ggcataaagc aaaatatata gtaaaaggaa ctttcttaca gttattttgg
     2521 aatgacaaca actttatata tttattaaat gtagattatc aaaatagtgg agaatctgaa
     2581 ttttatgaag atattttgat aaaaatagcg aaaagtataa aataattttt ttaagtgtcc
     2641 aagggtagcc tcttcattcg ttatacaagt atatatgaaa atgtgaggtg atttttatgg
     2701 ttttaaaatc tacatttacc tgagcgttaa aaatattgtt ataggaaaaa ttaatacatt
     2761 atagttataa catgcttgca ataggaataa aactttaaaa ttgaggtgta aaaaatgaaa
     2821 tcagatttat atttatgtaa aagtaataaa ttgtatagag acaacgaagt tctagaaaaa
     2881 tttatggaag atttaagttt tcttatagac gattataaat tctcgttaga aacaatatct
     2941 attctatgca aaataaatat tgagaaatta actaatttct atgcaggaaa aggatatata
     3001 ctgtatgaag aaatgtctct tattgaaagt gtacttgcac cttttttaag tgcaattgat
     3061 attgctaaac ataattatga tgttgtcatt aaatcgaggg aaagcgataa acctttgata
     3121 ttcgaaaatt aatttaaaac tgggggaata cacatgaaaa gattatttgc gttattactt
     3181 actttaagtt tagttatatt accatcaact ttagtatttg ctaatgaaaa agctgaaaat
     3241 gattcaattt atgctgaggc gccattagtg tttcaaagta atgaaaaagc atatacacat
     3301 ggtagtggag gaactgcata tataggttat atgtcaagta aacatcttaa ttggaggata
     3361 acatcttcaa aaggaatagt tatactattt tcaggaactg ttgagattag aaattcaaaa
     3421 aatatgcttg tagcatcata tccaataagt ggtggaggat cttcaactat atctggacaa
     3481 atagatgtta aatatcttaa aaaaggggat tataaagcta tattgaaagg tacaagtaca
     3541 acaggaggcg gagtcttagt agtaaaatct ggtctagttc agatgtttac aatttaatat
     3601 tataagtaaa ataaaaatca tgaatttatt gggggatagt cacaatacat attatttaca
     3661 gatattcata actattatag ttaaagacat tctattataa agaaaatgta taatttaaaa
     3721 ttataataaa aaaaattatc attagtgtcc aaaaatcact tcttcattcg ttatataagt
     3781 tatataaata gaaaacaaat gaagaggtga ttttatgaat cttagaaaaa cagttataag
     3841 tggtatgtgt gctttagtat gttcatcagc agttgcaata cctgcatttg ctgatgaata
     3901 tcaagataag aatattgaac aatattcagc agtaaactat atagatgata ctatggtgaa
     3961 aatgtcttat attaagtctg gtacttgcaa tttaagtatt tcaggaagta cagctaaagt
     4021 aacatgcttt gttagaggag catcaaatgt tacaagcaca tcaattattg ctagattaca
     4081 gaaaagcaat gataatggga aaacatggtc aacaatccaa acatggaatg cttcaggaaa
     4141 tatttcatgt tctctttcta agtcaaaaac tataggaaaa ggatattaca gagttcattc
     4201 cacaataaaa gctaactctg aaagtaaaaa tataataagt agtacaaaga aatattaaaa
     4261 atagatattg tatttatgag ttgtagtaaa caagaataat agttttttaa aagcaaatat
     4321 aattaatttt aaatagagtg ccaatataaa ataggtgtat aatatttata cttttttata
     4381 ttggcactct attttataaa ataagttatt atagaaaatg aaacataatt tatatataaa
     4441 ttttctgttt gacctggtat ataatctttt atatcacata tcagtatttt cccatcatta
     4501 taaccccaac caatcacagt tgcaggtatc atatcagctt ctccatcata aatgattgta
     4561 tgtttataca tgatttttcc ctcgctatta tctatagttt tattcaaaat accttctgct
     4621 attaatttag caactatgtc tttaatagtc tgtatctgct ttactgtcta caaaacatac
     4681 ttcaattaat atcgctggag cttttgtatg actaagccaa tacaattccc ttacgtccga
     4741 ttttgcacc
//
LOCUS       DAGQWR010000224         4722 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.227, whole genome shotgun sequence.
ACCESSION   DAGQWR010000224 DAGQWR010000000
VERSION     DAGQWR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.227"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     repeat_region   144..1359
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=343..371
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            2006..3277
                     /locus_tag="MIW95_003721"
     CDS             2006..3277
                     /locus_tag="MIW95_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBY3153013.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSIADTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPM
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     gene            complement(3916..4671)
                     /locus_tag="MIW95_003722"
     CDS             complement(3916..4671)
                     /locus_tag="MIW95_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HBY3153014.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT         1824 a          657 c          524 g         1717 t
ORIGIN      
        1 tattctactt ttatatttta atcattaaaa ctaaagtcaa tgttatctgg taattattta
       61 ctacaaatga taaaaaataa ttatatttta aagtagaaaa tgctgtataa attattgggg
      121 tattttacct atacttgttg caaatttaca taccacgtag ttaatataaa acaaatttca
      181 agcgattgga caacgaataa aaattgcaat ttatatatca tgtagttaat ataaagcata
      241 tttgtgtcga tatatagttc taactaagta taagatttac atacctcata gttaatataa
      301 aacaatgtgt ccgaattgga cacatagaaa ggaaaagtaa aaatttacat accacatagt
      361 taatataaaa cattatgata agttaaagca ttttacaaat actgatgaat ttacatacca
      421 catagttaat ataaaactat gtaagtcatt tagtgttgca gggtctggta agatttacat
      481 accacatagt taatataaaa ctctattaca acaatagtaa agtctataac aagattatat
      541 ttacatacca catagttaat ataaaacgaa aagaagacct agataaaaga atatgcgata
      601 tagatttaca taccacatag ttaatataaa actatgctta tatattatgt ttattctgtt
      661 gtttctaaaa tttacatacc acatagttaa tataaaacga ttttttcatg cggaggaaaa
      721 ttgatgtggg gacatattta cataccacat agttaatata aaacagataa tctaggcaca
      781 catacaatgc gcaaaacttt atttacatac cacatagtta atataaaaca ttcagacgat
      841 ataggcgcag gagctaagta ttctgaaatt tacataccac atagttaata taaaaccagc
      901 acgccaagta taagtaaaac ttccactatc accatttaca taccacatag ttaatataaa
      961 actaaatgtt gctccgtaac ctaaaaacat aatgctccaa tttacatacc acatagttaa
     1021 tataaaacgt gaaagaaaaa ttaatatgct atttacattt tacaaattta cataccacat
     1081 agttaatata aaaccattcc acaatttata caattttttc tgttctaaaa catttacata
     1141 ccacatagtt aatataaaac tcttcttatc ttccagttgc aaaatctatg cttgacctat
     1201 ttacatacca catagttaat ataaaactag tttgagtggt aaaagtacga agtacccctc
     1261 tttaatttac ataccacata gttaatataa aacatatgta attcaccgtt ttaaattata
     1321 gataaattaa atttacatac cacatagtta atataaaacc ccaaaataaa ctaagtattt
     1381 tcagtactta cacacataca actctctcaa atttgcagtg aaccatgagt agtgcaattg
     1441 ataacattta tcatacaccc tcaatccctt acattgcaac tgttaaaccc tactttatca
     1501 aaaatatcgc tcactgcaaa atttatatat ttttattata tcataaaatt caaactataa
     1561 aattaaaatg gaataattta tcaatttaag aacacctaca tatttgtaaa tacttactat
     1621 attatctatt ctaataattc ttcttctagc tggaactaat ataaaaatca tctctgaaag
     1681 attaggacat actaatataa aaataactat gaatagatat tttcttgttt taaacgatat
     1741 ggataaaaaa gcttttgaaa atataatcaa agttctcttt aaataacatt ccattttatg
     1801 taagtaagca ttatattttt cttaaatgtc acttaagtgt cagttatata taataatagt
     1861 aaacaactgt atttcactaa tatttgctat tatctaatag tatttatagt ttagatttga
     1921 tataatacat gtggtaaaat tttgatttgt aaaacaaaat tttacctaca tatattaaaa
     1981 caaatttaaa actaaggaga cctatatgtt aaaagtatta aaaggaaata taatttttac
     2041 aaaaacttca gaaactttca cagtatttga aaatagttat attatactgg aaaaaggtaa
     2101 agtaaaggga atatttaaag agatacctaa ggaatacaaa aacctaaaag tagtagatta
     2161 cactgataag cttataatac ctggtatgaa tgacctccat gcccatgcct ctcaatttaa
     2221 aaatcttgga atggctatgg ataaagagct tttgccatgg cttgaaactt atacatttcc
     2281 agaagagtca aattataaag atatagaata tgcaacaaaa atgtataaaa aatttgtaaa
     2341 agaactttgg aaatctggaa ctacaagaat agcagtgttt gcaacagtac ataaagaagc
     2401 ctctatgaaa cttatggata tattcaaaga agttggtctt ggagcatacg ttggtaaagt
     2461 aaatatgaat atgaattgcc ctgatacttt attagaaaac actcaggatt ctattgctga
     2521 tacagaagaa attataaata agtatagtga tacagattct attgtaaaac caataataac
     2581 acctagattt ataccaagct gtacaagtga acttttaaaa ggtttaggag atttatgttt
     2641 aaaatataat attcctatgc aatcacactt atctgaaaat tatgatgaga ttgaatgggt
     2701 tagaagtcta gaacctgaat ctaaattcta tggagatgca tataatagat ataatttatt
     2761 tggtcaaact cctactttaa tggctcattg tgttcattgt tctcaggatg aaatagattt
     2821 aatgagagaa aacaatgtta tggcagttca ctgtccagct tctaacttta atgttggtag
     2881 tggtgctatg cctatcagaa aattaatcga taataatatt agattagcac ttggatcaga
     2941 tataagcggt ggacatacat tgtcaatatt taaagcaatg gtatctgcaa tacaattatc
     3001 taaattatat tgggttaatt ctggtaagaa gtataatttc ttatctttat ctgaggcttt
     3061 ttatattgca accaaaagtg gtggaagttt ctttggaaaa gttggtagct ttgaagaagg
     3121 ttatgatttt gatgcattga ttattgatga ttcaaattta aatcatgaca attattctat
     3181 acttgaaaga cttgaaagat ttatctatgt tggtgatgat agaaatatca ttcatagata
     3241 tgtatgtgga aaactaattg aagaacctaa tatataatta taataaaaat tttaattata
     3301 tattatggtt aatttatgca actataatta ataaaagagt atattttaaa tctaaaagct
     3361 ataggttgaa acatactctt tttattttgt ttatttatag ctttagttat taatcatata
     3421 taaatctaca aaacgtattc ctagtaactt atattttttg atatctacaa tcataagact
     3481 atattattgg attcatacct caaaaatatt gccttctgaa cattattata ttcaaagtta
     3541 tgttaattta caatgatata tctaacatgt attgattaaa aaaaattaaa acttgctttt
     3601 catctcctat atctaaagaa gtggcattta gaataaacta ttgtaatatt aaagttgaaa
     3661 ttttctagtt tatttactct atagcaccat attttaatac tattaagtaa aaatgtagtt
     3721 aaaatatata ctaatatccc tgaaataagt atttcttttt tatttagcac acttattcgt
     3781 aaggtattag ttattatatt ttcaaattat agaataaata ttatacttat tgctaattat
     3841 aatattaata ctgttaagat atttttcttc atagttatac ccattattta atacaattta
     3901 ttataaattc aatttttaaa cttttcttat ctccttatca gaataaaaat cccctttact
     3961 tccttttatt tttactattt ttaaacttgc catcttctct aatctccaaa gtataaacca
     4021 atcttttaca cgcatttcat cttcaaacat acagtttgca accacttcaa ttaaaggttg
     4081 ccatttgtca ctgatatttc ttaatatata tttatcaaaa taagtttctt caacactttt
     4141 aatttcgtta cctactaatg ctctaaaaat agaattttct ttaactaaca catcccattt
     4201 atttttaaat tctagttttt cattaaagcc aatttctagt gtcttatcta aaaagaatgg
     4261 aaaatcttct ggagacattt cactggtaaa tgaatatcta attagagcat cttctgtctt
     4321 tttttcatct actaatgcaa tctttatatt tttatcttga aacttactca ctacataata
     4381 tactccacac aaatcttttg ggttatttgc acaccatatt attatatttc tacattttga
     4441 taatttccta tcttctaaca aatctggctt gaataatagt ttcggattat tttcaaaaac
     4501 atttctaatc ttaaaaatat tttctcttaa ttcgtagtta tttacatcaa aaatattacc
     4561 tattgataaa tcatcttcaa ataatacaat tttattatgt ggtattaact ttaacttcat
     4621 tgcttctttt aataacaaat aacaaatatc ctcaaagcat atatgtgcca taaaattcac
     4681 ctcttttaaa attaacaatt tattttatta taatatataa ac
//
LOCUS       DAGQWR010000225         4641 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.228, whole genome shotgun sequence.
ACCESSION   DAGQWR010000225 DAGQWR010000000
VERSION     DAGQWR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.228"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            277..1752
                     /locus_tag="MIW95_003723"
     CDS             277..1752
                     /locus_tag="MIW95_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBY3153015.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1839..2252
                     /gene="speD"
                     /locus_tag="MIW95_003724"
     CDS             1839..2252
                     /gene="speD"
                     /locus_tag="MIW95_003724"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBY3153016.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2282..3133
                     /gene="speE"
                     /locus_tag="MIW95_003725"
     CDS             2282..3133
                     /gene="speE"
                     /locus_tag="MIW95_003725"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBY3153017.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3123..4001
                     /gene="speB"
                     /locus_tag="MIW95_003726"
     CDS             3123..4001
                     /gene="speB"
                     /locus_tag="MIW95_003726"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBY3153018.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4181..4381)
                     /locus_tag="MIW95_003727"
     CDS             complement(4181..4381)
                     /locus_tag="MIW95_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBY3153019.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1874 a          507 c          780 g         1480 t
ORIGIN      
        1 aaaaagtaaa aaaagcaaaa taataaaaaa taagaaagtt tataaatact aagttttaaa
       61 aaaattaata taaacaacaa aaaaataaaa agtttataaa tattgatata gctaatgtat
      121 atgtttttga gtaacttcaa aaaaacactt gtaaaaatgc tggaatgtta atataataaa
      181 tatcatgagt ttaataaaag taaaaataaa aaatagaaat aataaataat aaatattaaa
      241 aatatttgaa tatagagagg agatgaattt gaaatcatgg atagcgagtt tgcaaatgca
      301 gttgaattaa gctatgagtg tgctccatta ttagagtcgt taaaggaata ctcagagaaa
      361 gatatagctt gttttgatgt tcctggacat gtaaaaaata gaggtgtagc tatattaaat
      421 aaatatctag gtgaaagtct catgaaaatg gatataaatt catcaccaac tatggataat
      481 gtatctgcgc caaatgggat tataaaaaat gcacaagatt tgctagcaca agcatatatg
      541 gctgatgagg cattctttat aacgaatggc acaactcaag caatacatgc aatgattttg
      601 agtgttataa aaccaggtga aaaagtatta cttccaagaa atatacataa atcagtaata
      661 aatgcgttaa tactttgtgg aggaatacca atatttatac aaccagaatt tgatgaaaaa
      721 ctaggtataa gtttaaatat tactcttgaa aaagttagaa cagagataga aaaagattgt
      781 aatataaaag cattattttt ccttaatcca acttactatg gagtttgtgc agaccttgag
      841 agtattatag aactatgcca taaaaataat gttttagtgt tagttgatga agctcatggt
      901 gcacattttc catttcattt agatttacct ccttctgcaa taagtttagg agctgacatg
      961 gtagctgtaa gtatacataa aactggagga gcattgacac aatcttcagc actcttattg
     1021 aatagagata atgtaagttt tgaaaaggtt cttcaatcaa tcaatatgct acaatcaaca
     1081 tcagcatctt atttgcttat ggcaagtata gatggtgcta gagttaatct agttgagaat
     1141 ggtgaaaagc aactgtcaaa agcattaaat ctatctaggt atgcaaaatc taaattgaac
     1201 aaaattgatg gaataaaagt tttatctaca gaaatattga aacaaaaagg tgttaaattt
     1261 atagatgaaa caaaattgtg tataaatgta aaagaattaa atttgactgg atttgaagta
     1321 tacgatttat tatataaaaa tttttcaatt caagtagaat tgggagattc atataatata
     1381 ctggcacttg tgtccattgg aacaaataaa tcagatatag atagattagt aaaagcactt
     1441 tctataattg caaagattta tagaaaagaa tcaacattaa atgaatttaa tatggttcaa
     1501 ataaatccaa ttattaaact taatccaaga gaagcttttt atgcaccaaa ggaaagtgta
     1561 gaaatcaatt catgtataga tagaatttgt ggagaatcaa taatggcata tccaccaggt
     1621 ataccgatta ttgctccagg agaattgata acagaagaaa ttatggagta tattattttc
     1681 ttaaaaaata gcaatgcgta tcttacagat gtacaagata aaaacttaga tagaatacta
     1741 gtaataaaat aaattacttt attttgcaag ggcaagtgtt tgttaagtag agtttatcaa
     1801 atagaactta ttaaaagtat aaaggagttg gggttgaaat gaaatttgaa acactaggaa
     1861 gacatatact agtagaatac tataattgtg atgaaaacgt acttaaagac ccacgtctta
     1921 tagaaaattt tatgaatgat tcagcattaa atgcaaaagc gacaattgtg gattctgtat
     1981 tccaccattt taatccttgg ggggtttcag gagctgttat aattgctgaa tctcatttga
     2041 caatacatac atggcctgaa tatggttatg cagcagcaga tttttttact tgtggtgata
     2101 tagacccatg gaaaagtttt gaattattag agcaactttt aaagtctgaa agaagcgagt
     2161 ctacagaaat tccaagaggc ttaactacaa aaattaaaaa atattcaaaa aaagatttag
     2221 gtaagattac tcataaacca gaagcagtat aaaacataca agagaaagtg aggattatta
     2281 tatggaactt tggtatacag aagaatggac agaaaatgta agattttcta taaaagtaaa
     2341 taaacatcta tttgaaggaa agtctcaatt tcaaaggata gatgtatttg atagtgatga
     2401 atttggaaaa tttctaacaa tagatggatt aatgatggta acatataaag atgaatttat
     2461 ataccatgag atgataactc atgttcctat ggctacaaat ttaaacataa agaaagtatt
     2521 agtcataggt ggaggtgatg gaggtacagt aagagaatta agccgttatc ctcaaataga
     2581 aaagattgac atggttgaaa tagataaaat ggttgtagat gtatctaagg agtatatgga
     2641 tatatgctca tgtaaattag atgataaaag agtgagttta tattttgaag atggtgttaa
     2701 ttttgttaag tgtgcacatg ataaatcata tgacctgata atagtagatt caactgaccc
     2761 aataggacca ggcgaagggc ttttctcaac tgatttttat aaggattgtt atagaatact
     2821 gactgatgat ggaattttag taaatcaaag tgaaagtccg tattttgact ttaatgctaa
     2881 agagatgaaa agagctaata aaaaattaaa acagatattt ccaatatcag aagtttatca
     2941 agcacatata ccaacatatc catctggtca ctggttgttt ggatttgctt caaaaaaact
     3001 taatccagta aaaaatcaag atagaaatgg atgggagaaa ttaagtttaa aaactaaata
     3061 ttataatagt gatatacatt taggttcgtt tatgttacct caatacgtga aggagatgct
     3121 agatgaagaa taatttctat catatgaaca cttttatgag tatggacaaa aattatgaag
     3181 aatctaatct tatagtattt ggtgttggat ttgatggaac tacttctaac agacctgggg
     3241 ctagatttgc aagtagctct atgagaaaag aattttacgg tcttgagaca tatagtcctt
     3301 ttttagattt ggacttagag gattataata tatgtgatta tggagattta gaaattagtg
     3361 ttggaagtac agaacaagtc ttaaaagaaa tctatcaaga gacatataag attgttagag
     3421 attcaaaggt accctttatg attggaggag agcatttagt tacattacca gcctttaaag
     3481 cagtacatga aaagtacaat gatatatatg taattcattt tgatgcccat actgatttga
     3541 gggaagaata taataatagt aaaaattctc atgcaacagt aattaaaaga atatgggata
     3601 ttgtaggtga taataaaata tttcaatttg gtataaggtc tgggacaaaa gaagaattta
     3661 aatttgctac agaagaaaaa cacacataca tggaaatagg aggaatagat acgtttgaaa
     3721 atatagttaa tatgctaaat ggaaagaata tttatctaac tatagattta gatgtattgg
     3781 atgcatctgt gtttccggga acaggtacac cagaacctgg tggtgtaaat tatagagagt
     3841 ttcaagagat ttttaagatt ataaaaaact ctaatataaa tatagttggt tgtgatattg
     3901 tagagttaag ccctgattac gatacaacag gtgtatcgac agttatagct tgtaaaatct
     3961 taagagagtt atgcttaata atatctgata aaattaaata gagtatttta tataaatagt
     4021 atatattata atataagaag ttattttaaa aaagttagtt taagtttgac gcttttctaa
     4081 agtaacttct tatttttgta attaaaaata aatataataa acaacaaaaa agagccctcg
     4141 atagctcctt ttctaaaata tatatattta aattaaataa ttatattcta tttacgtttt
     4201 cagcttgagg tcctctatta ccttgagtta tatcaaagct aaccttttcg ccttcttcta
     4261 aagttttgta tccatcattt tgtatagctg agaaatgtac gaaaacatca tcttctccct
     4321 ctacagatat gaacccaaat cctttttcat tgttaaacca ttttactatt ccgtttttca
     4381 ttataagaaa acctccgcat tattaaaaaa tacaaactaa aaaaataagt ctgttctatt
     4441 aatataacat aaattttaaa taaaagcaaa taaaattttt aaaaaaatta attactttta
     4501 gaatttaata aaaattgtta aattatacag tgagaagtat tgtaaaattt gacgaaaatg
     4561 taaatgtaga taataaattt aatgtcaaaa aatgcaacaa aaaggtatgt aagtaaaaag
     4621 taaaattaaa atatataata a
//
LOCUS       DAGQWR010000226         4621 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.229, whole genome shotgun sequence.
ACCESSION   DAGQWR010000226 DAGQWR010000000
VERSION     DAGQWR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.229"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     misc_binding    158..392
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            442..1461
                     /gene="pheS"
                     /locus_tag="MIW95_003728"
     CDS             442..1461
                     /gene="pheS"
                     /locus_tag="MIW95_003728"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBY3153020.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            1478..3871
                     /locus_tag="MIW95_003729"
     CDS             1478..3871
                     /locus_tag="MIW95_003729"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBY3153021.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            3896..4486
                     /gene="zapA"
                     /locus_tag="MIW95_003730"
     CDS             3896..4486
                     /gene="zapA"
                     /locus_tag="MIW95_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBY3153022.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDVYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         1832 a          525 c          933 g         1331 t
ORIGIN      
        1 ttagatgtat attatataaa aaataattga gttaattatg cattagatat atattatata
       61 aaaaataatt aaattaatta tgtattggat atatattaaa aatagtcaca taatttgaat
      121 ggaaatgata taatactaaa ataaacaata taatattgta aatgcaatga aagaggaaag
      181 tattttgatt aaacttagta aagagataaa cacctaggct gggagtgttt tctaagaggt
      241 catgagaagt tccctctgga gtaacagagc tgaaatttta cagtaggctt tgacgtcaaa
      301 aacgcgttaa gtttgttaga ggtggtttga tgatttttaa attgttaaac tactagggtg
      361 gtaccgcgaa actatagcct ttcgtcccta gacagggatt gaggggcttt ttttatacaa
      421 aaaaacgaaa gggtgatatg tgtgcaagaa aaattacttg ctttacgtga agcagctttg
      481 gctgaaataa aagaagcaca aagcatagaa agtgtagaaa gtttaagagt taagtactta
      541 ggaaaaaaag gtgagataac tgccatactt aaagaaatgg gtaaattatc tgctgaagaa
      601 agaccagtag ttggtaaggt tgccaatgag gtaagagaaa acattgaact tagcataaat
      661 tctaaaaaag aagaaataaa tgctattgaa aaagaaagaa aattaaaaga ggaagtgata
      721 gatgttactc aaccaggaaa agttttaaag gttgggaaga agcatccaat aactcaaatt
      781 atagatgaag taacagatat atttatcgga atgggattct ctatagcaga agggccagaa
      841 gttgagactg ttgaaaacaa ctttgacgca ttaaacgctc ctaaagacca tccatcaaga
      901 gatatgagcg atacattcta tatcaatgat ggggtattac ttagaactca aacatctcca
      961 gttcaagtaa gaactatgag aagtcaagag ttaccaataa aagtaattgc accaggtaga
     1021 tgttttaggt cagactcgcc agatgctaca cactcaccaa tgttccatca gatagaaggg
     1081 cttgttgttg gaaaagatgt tactatggca gaatttaaag gaactatgga tatcttcgtt
     1141 gaaaaattgt ttggttctga tatcaaaact aagtttagac ctcacaactt cccatttaca
     1201 gaaccaagtg cagaggttga tgttacttgt ttcaaatgtg gtggtaaagg ttgcccaatg
     1261 tgtaaatatg aaggttggat agaaatatta ggttcaggta tggttcatcc aaatgtgctt
     1321 agaaattgtg gaatagaccc agaagtttac agtggatttg catttggagt tggggttgaa
     1381 agacttgcaa tgcttaaata cgaaatagat gatattagat tattattcga aaatgatatg
     1441 agattcttaa atcaatttta attaggaggg tatgtagatg ttagtatctt taaaatggct
     1501 tagagactat gttgatatag acatggatgt aaaagagttc gctgataaaa tgacaatgac
     1561 aggaactaaa gttgaaacaa tagattatta tggtgaagaa atagaaaata tattggttgg
     1621 aaagatttta gaaataaaac aacatccaaa tgctgataag ttggttgtaa ctaaagtaga
     1681 tattggagat aaagttgttc aaatagttac aggagctaca aatatatcag aaggagatta
     1741 tattccagta gctgtaaatg gttctaagtt acctggagga gttgaaatca aacagactga
     1801 tttcagaggt gaattatcag atggtatgat gtgttcagca gctgaactag gtatagatga
     1861 acattacatt gaggagtata aaagaggtgg tatatatatt ttagaccacg aagattctta
     1921 tgaattagga aaagatataa aagatgtttt aggattaaaa gatgctttaa tagattttga
     1981 attaacttca aacagacctg attgtaaatg catgatgggt atagctagag aagcagctgc
     2041 aactatagga acaaaagtaa aatatcctga aatcgaagta aaagaaagtg acgaagagat
     2101 agatttcaaa gttgagatag ataatccaga tttatgtaga agatatgttg ctagaatggt
     2161 tacagatgta aaaatagaac cttctccata ttggatgcaa agaagactta cagaagcagg
     2221 agtaagacct ataagtaaca tagtcgatat aacaaacttc gtaatgttag agcttggtca
     2281 accacttcat gcttttgata taaatcaagt agagactgga agaatagtag taagaaatgc
     2341 taaagatgga gagaaacttg taacattaga tgatgttgag agaacattag ataaagatat
     2401 gctagttata acaaatggag aaaaatcact tggtttagct ggtgtaatgg gtggtgctaa
     2461 ctcagaaata acttctaata cgaagactgt actttttgaa agtgccaatt tcaaaccaga
     2521 aaacataaga atgacagcta aaaaagttgg tattaggtca gaagcatctt caagaaatga
     2581 aaaagactta gaccctaatc ttgcagagat agcagcaaat agagctgcac aacttgttga
     2641 aatgttagga gcaggaaaag ttttaaaagg tgttgtagat gtatatccaa ataaaccaga
     2701 acctaaaaaa ttggtagtaa atcctcaaag aattaaccac ctattaggtg tagatgtacc
     2761 aatggagcag tttgtaggaa ttttagaatc attagagttt aaatgtaatt tggtagctaa
     2821 tgataaatta gaaatagatg taccaagctt tagaacagat atggaacaag aagctgatgt
     2881 atgggaagaa atagctagaa tttatggatt tgagaatata ccttctgtac aattagaagg
     2941 aaatacgaca gcaggaatta aaacttcaaa acaaaaattc atggatgctt taaaagataa
     3001 ttcaactgct gtaggattaa atgaaatatt aacatattca tttgtaagcc ctaagggagt
     3061 cgataagata agagtaccag aaggtaatgc taagagaaac tttgttaaat tacttaaccc
     3121 attaggagaa gaaacttctg taatgagaac tactttaata ccaaatatgt tagatgtttt
     3181 atcaactaat gtatctcata aaatagaaga agtatctgca tttgagtgtg gacatatatt
     3241 catcccacaa gattcagaat tacctaaaga agaaaataga atgtgtgtag gtatgtatgg
     3301 taaagatgtt gacttctttg cactaaaagg aactatagaa actatacttg taaatgtcgg
     3361 atttaaaggt tatgaaattg aacctcaaga taataatact accttccacc ctggtagatg
     3421 tgctaaaata gtgtataata ataaatatgt aggtacatta ggtgaattgc atccagatgt
     3481 tatagaaaac tacaatctag gtcaaagagt ttatgtcgct gaaatagata ttgactttgt
     3541 atttgataat tctgatagaa caaagaatta tgtgccatta ccaaaatatc catctacatc
     3601 aagagatata gctcttattg ttaaagatga tgtatttgta aaacaaatag aagatataat
     3661 aaaggaaaat ggtcaaggat tagtagaaag ctacaagtta tttgatgttt acaaaggttc
     3721 tcaaatagaa gctggataca agtcaattgc gtattcaata acttatagaa gtaaagataa
     3781 aactctaaca gatgaagatg tagctaaggt gcatgataag atattaagtg aattaagcga
     3841 aaaattaaat gctaacttaa gatcaaatta acaactaatg tcgggggagg ttaggatgaa
     3901 caaagtaatg gttaaaatcc atggcgctga atatccaatg gttggagaca aatcagagaa
     3961 gtttatgatt agtattgctg attttgtaga caaagaaatg gataaaataa cacgtcaaaa
     4021 cccgaaattg agcttatcag ttgctgcgat actaactgca ttaaatatat cagacctttt
     4081 atttgaatgt tcagatgaga atgagaagtt gattaaagct aatgaagaat tgagtaagaa
     4141 ggttggagca tctaatgaag agcttcaatt agaaatcaag tctttaaagc ttacgatagc
     4201 agaaaaagaa gcagaaaatc gtgaagctga gactaagatg aaagagttaa ttgagattat
     4261 agaaaataaa aagcaagaaa tattcgaact tagtaatacg acagaaggtt ctagagcaga
     4321 gttagatgtt tataaaaaca agattgagga gttaagtact caacttgaag aagcaaatga
     4381 aagagctact atagctgaaa acttagcttc tgagtttcaa aacaaagctt atgatttaca
     4441 gttaaaatgt acagggttaa ataatgatgc aaaaaatgtg gaataaacta aaagatttgc
     4501 tgtctcttgt tttttgagac agcttctttt aatgtataaa tataaatgct atataaatat
     4561 agctaatgtt taaatgtaaa taaatataaa tgctatataa atatagataa tgtttaaatg
     4621 t
//
LOCUS       DAGQWR010000227         4577 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.230, whole genome shotgun sequence.
ACCESSION   DAGQWR010000227 DAGQWR010000000
VERSION     DAGQWR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.230"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            230..1381
                     /locus_tag="MIW95_003731"
     CDS             230..1381
                     /locus_tag="MIW95_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBY3153023.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1427..2602
                     /gene="thiI"
                     /locus_tag="MIW95_003732"
     CDS             1427..2602
                     /gene="thiI"
                     /locus_tag="MIW95_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBY3153024.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2778..3581
                     /locus_tag="MIW95_003733"
     CDS             2778..3581
                     /locus_tag="MIW95_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBY3153025.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGIEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3824..4465
                     /locus_tag="MIW95_003734"
     CDS             3824..4465
                     /locus_tag="MIW95_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBY3153026.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1822 a          441 c          893 g         1421 t
ORIGIN      
        1 tattatttta tcatatattg tattttataa tatatttttg taaggtatag tataagtata
       61 ttatatttta ctataaaaat ttgtaataaa gtgaaaaaac tagcttttca tgaaataata
      121 ttttataatt taagattgtc tttttaataa agattaacaa aaaaaatact ataattagtg
      181 gtataatagt tagagttgaa gattttaaat aaaggtaggt gtaattaagt tggaaatata
      241 cttagacaat agcgcaacta caaaacctta tcaagaagtt atagataaaa tggtttatgc
      301 acttaatacg gaatatggaa atccttcttc tgtccataga aaaggtgtag aagttgaaaa
      361 agctataaaa gaagtaagac aagatatagc taaatctcta ggagcgaaag aaaaggagat
      421 ttattttaca tcaggtggta cagagtgtaa taatactata ataagaggta ttactagttt
      481 aaataaaaaa agaaaaaatc acataatatc tactaacata gaacatcctt ctgttttaaa
      541 tacattaaaa gatttagaag aagatggttt tgaagtaact tatttagagg tcggaaaaga
      601 tgggaaaata aatatagagg atttaaaaaa tgcgataaag tcaactacat gcttagtaag
      661 catgatgcat gttaacaatg aaataggaac tatacagcca ataggtgaag ttggaaagta
      721 tttgaagggt ttaaaagaaa aaatttattt ccatgttgat gctattcagt catatggaaa
      781 aattaatttt agaccatcta aatacaatat agattttatg agtgtaagtg cacataaatt
      841 tcatggtcca aagggaattg gatttatgta tataaaagaa aataatcgcc ttaagcctat
      901 gttaacaggt ggtggtcagg aaattggaat taggtcggga acagaaaatg tacctggaat
      961 atatggtctt ggagaagctg ttagaatact aaacaaagat ttagatgttg ttatttcgaa
     1021 agtaaataac ttaaaaaata tattaaaaaa tgaaataatt gataatatag aagatataaa
     1081 aatcaattct ccagaagatg gagtatgtca tatattaaat gtatcattta gagggacaaa
     1141 aggagaagtt ttacttcatt acctagagca aaaaggtatt tatgtatcaa ctggctctgc
     1201 atgttcttct aaaaagaaag gtagttacgt attaaatgct ataggtctta caaatgaaga
     1261 aataaatgga acaataagat ttagtttatc tgatatgaat acagaagaag aaatgttaga
     1321 agctgtgaaa gttttgaaag aatcaatttg tgacttaagg tcaataatga aaagaaaata
     1381 aagaatactg tatagatagt gataataaat tagtttaaga ggtgaaatgg tgtataatat
     1441 attaatagta aagtatggag aaattggagt aaaaggaaag aatagatata tatttgagaa
     1501 taggcttata agaaatataa gaaatatgtt aaaaccaatt ggaagattta atgtatataa
     1561 agagtatgga agaatatatg ttgatttaga agactatgat tatgaagaag taattgaaga
     1621 agttagaaaa gtatttggga tagtgggtgt gtgccctgga gtaagagcta aaaaagatta
     1681 tgatacatta aaagaaatag ctttaaaaat gttagaagaa aaaatagagg ctggatataa
     1741 aacttttaag gtagagtcaa gaagaggaga taagtctttc gggttgactt ctcaagaaat
     1801 gagtatggat ataggtggat acttattatc aaaggtagga gataggatta atgttgatgt
     1861 aagaaatcca gaagttaaaa taaagtgcga atacagagaa tttcatacta tggtctatag
     1921 tgatactata cctggatatg gtggattgcc acttggaacg aatggtagag ctatgtctct
     1981 tttatcagga ggtatagata gtccagtagc tacatggatg gttgcaaaaa gaggtatgga
     2041 ggtagaggca gttcacttcc atagttatcc atttacaagt gaaaggtcac aggaaaaagt
     2101 taaggattta gctaaaatac tggcaaaata ctgtggtaga gtaagacttc ataaagttaa
     2161 catattagaa atacaaaaag caattggtga gaattgtaat gaagaagaag ctactattct
     2221 ttcaagaaga tttatgatga ggatagcaca gagactttct gaaaaaagac attgtgatgc
     2281 tttaattaca ggtgaaagta ttgggcaggt tgcatctcaa actatacaag ggcttacttg
     2341 tactaatgcc gttgtagact taccagtgtt tagaccactt atagcaatgg ataaatcaga
     2401 tatagttgat atagccaaaa aaatagggac ttttgaaaca tctatagtgc ctgaagaaga
     2461 ttgttgtagt gtattttctc ctagaaagcc tgtcactaaa cctagattag agaaaataga
     2521 aaaatctgaa acagcacttg atattgaaaa attagtacag gatgctattg acaaaataga
     2581 ggtagaggat attgagtttt agcatacaag ttatttaatt actaaattga atattgagta
     2641 tattagtagt tctgtaagta tattagtagt tctgtaagta tattagtagt tctgttgaaa
     2701 ggatttatgt agacaattta ttaatatata gtatttaaaa ataagtacta taattttatt
     2761 taatgggaat gattattatg aataaaaagg gttttacatt aattgaattg ttggtagtta
     2821 tatctataat aggaatttta gttatagtag ctgttccagc gttatttaga aatatagaaa
     2881 aaagtaaggc agttacatgt ctttctaata gagaaaatat aaagactcaa attgttattg
     2941 caatggctga ggaatcaagt aaagacaaga atgaagtcat aaaagaggta ttagaaaaca
     3001 aagatggtaa gtactttgaa acagaaccaa agtgtaagtc aggtggaata tattcagcaa
     3061 cgtttgatga tggttatgat ggaataactg gaatagaaag cattgcaaaa gtgtatgtta
     3121 cttgtacaaa acatccagat ggtattgaaa tggctaggga tatacatcaa agtatgaaag
     3181 atttgattgc atcatttgca caagaccctt ctataatacc aggagcttca aagggcaatg
     3241 atgattttag aaaatattta ttagacaata aatataaaaa tgggtggcct acaattccag
     3301 atgaatttaa ggcaaaatat ggattaagta aggatacact atatatacaa ccatatgcat
     3361 ataatcctac taaatctgat gctactgtag ttgtatttgc aaataataag actggaggta
     3421 attggtatac ttccctagtt tacgattatg atgaaggtag atggtataaa ggtaaaaatg
     3481 gtatttctgt tgcaggtagg tcatgggatg ttgacacaga tagtgttaag tctgtaaaaa
     3541 cagagattca ttctaaagag ggatggggtc ctttaaatta atatatcatg ttttattaat
     3601 ctggtattga ttaaactata gaaaagagca gtttctatag atgacgaata ccagattttc
     3661 atttatatgt ataattaaga tatattaaaa gtatttatat ttttaagtct tcacatacaa
     3721 gctaaagata attataataa tattgtaaag aaatttaatt ataagattaa ttttctgaag
     3781 atttagtatt tgtactaaga aatacataaa aaggggaata gatatgaata ataattttca
     3841 agacaaccaa acaataaatt taatacaaag tcgtagaagt atacgaaaat ttacaacaga
     3901 gcaaattagt gatgagcaag taaacacatt attacactgt gcatttgcag caccttcagg
     3961 atgtaataaa cagccttggc acataactgt tgtgcaagac cagaaacttt taaaagagat
     4021 tagtgatgat actttatcta ggattcatga agtgagtaat gttgagataa ataaaaactt
     4081 taagttattt tatggtgctc caaccgtttt atttatttct tatgatgaaa gtagttcatg
     4141 ggctccatat gatataggaa tacttacagg gaatataaca acagctgcac aagcactagg
     4201 gcttggaagt tgtataatag gaatggttag aggtttattt acacctgttg aacaagggga
     4261 tattgaaggt cttgtgagtg tattggataa agaagatgta aaggaatcag agtcaataaa
     4321 gatgaagttt gatacaaata aaaaatacag agaactactt gatataccag aagggtattc
     4381 tgtaccattt ggtattgctg taggtattcc agatggaaat ttaccaaatg ctagagaggt
     4441 agtttacaaa gtttccagag tttaggataa agtattgcaa gaaccaatca gttacttgaa
     4501 gaattaaacc agtaatatac aaataaattg atggaagagg aagctattta aaaagtagtt
     4561 tagatttttc attgtaa
//
LOCUS       DAGQWR010000228         4444 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.232, whole genome shotgun sequence.
ACCESSION   DAGQWR010000228 DAGQWR010000000
VERSION     DAGQWR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.232"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..749
                     /locus_tag="MIW95_003735"
     CDS             <1..749
                     /locus_tag="MIW95_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBY3153027.1"
                     /translation="EYNYVAIERISEKTYKMKCETTGPVFELGKLIPIEYIDGLETAE
                     LTEILINGEDEESEDSLRQRYYDSLNSQSFGGNIQNYRDEVNKIQDVGGVKVYPTWDG
                     GGTVKLVIINSNFKVPSEELVNLVQEEIDPIGHQGEGLGLAPIGHRVTVEGVTSTTIN
                     ISAEITYKNGYTWENIKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGV
                     LDIANTMINDVKSNLTIDSNSIVVRGEVVG"
     gene            742..1359
                     /locus_tag="MIW95_003736"
     CDS             742..1359
                     /locus_tag="MIW95_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBY3153028.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            1371..2396
                     /locus_tag="MIW95_003737"
     CDS             1371..2396
                     /locus_tag="MIW95_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBY3153029.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTELKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNINNIELKIDPTIIFATLKDIQDLDAK
                     ISNVNTKIDTTKTELTSNIETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWIDNQEGGFKATVTHGLLTQRITVSIIDATTKDNVVPDFTIIDDNSIEVRSGVKV
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIHEEIGKVAEQLTGINSNIISTV
                     NNDILPI"
     gene            2413..3939
                     /locus_tag="MIW95_003738"
     CDS             2413..3939
                     /locus_tag="MIW95_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153030.1"
                     /translation="MALSMSYFNLPDKRKYTKNLAFNPFAGGRQNFEWTGGDHGLNGE
                     FKETCLSCTYNGSTLNWGSGNVWVLGEYGQYTFTYNCESMHVDTQQKFPYTSNRIITI
                     KGRPVISGSDTSLGNKRKGFSVDFTVSDDTPNVNLIVRAYLDDKLIQNITPVVQNSTL
                     TATVTDSQLNSLSVDGNHKLKIQLNDGYDNFDRIFTFKKIEKGIDISTSLVTDSQAKF
                     TVTKIYSELTKIECYLDETLKETFTTDLYSEKTINYELIDNAIHTLKIVVTDAENVVE
                     EKVISISKNIMPLQPDATLQDISTKLTEIGQGVRNGKTSIINTLALKNIDASLNNTLV
                     ELSEKIKGGFDSGDASLQDLMNQLTQANNTISQLNTKYKVASGTVTSFADSTKIAYPY
                     LTDNVTKPGSWIKVSNLGFKPNIFFADFDYYDAEYKNNYKLFLFACNGVATQRGVDFS
                     SVTSFIRKSGDEYFHANGWLYSNSEGDVYFNNTGVQIPAYNFDSTQKHTYKWYAIKFI
                     "
     gene            3954..4247
                     /locus_tag="MIW95_003739"
     CDS             3954..4247
                     /locus_tag="MIW95_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153031.1"
                     /translation="MNVPNRIIYDQTGRTIFETGESCGDVLPHYTITELHYIDIEYGS
                     IDYTRNRVIGINIETKEPILEEIPVYITDEEKRIQELENQLLIAENEKVGGLL"
     gene            4247..4429
                     /locus_tag="MIW95_003740"
     CDS             4247..4429
                     /locus_tag="MIW95_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153032.1"
                     /translation="MNINNVVVRILAERILSKGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGAEPTI"
BASE COUNT         1806 a          517 c          829 g         1292 t
ORIGIN      
        1 gagaatataa ctatgtagca attgagagaa tatctgaaaa aacatataaa atgaaatgtg
       61 aaactactgg acctgtattt gagttaggaa aactaatacc tattgaatat atagatggtc
      121 ttgaaactgc tgaactaact gaaatcttga taaatggaga ggatgaagag tcagaagata
      181 gtttaagaca aagatattat gatagcctaa attcacagag ttttggcggg aatatacaaa
      241 actataggga tgaagttaac aaaatacaag atgttggagg agttaaagtt tatccaactt
      301 gggatggagg tgggactgtt aagttagtaa taattaactc taattttaaa gtgccaagtg
      361 aagaattagt aaacttagtt caagaagaaa ttgacccaat tggacaccaa ggagaaggct
      421 taggattagc accgattgga caccgagtca cagttgaagg agttacaagt acaactataa
      481 acatatcagc agaaataaca tacaaaaatg gctacacttg ggagaatata aaatcaattg
      541 cagaagaagc aatagacgac tatttaaatg aacttaacat gagttgggaa gatgaagaaa
      601 acttaatagt ccgtatatct caaattgaaa ctagattact tagtattgat ggagtattag
      661 acattgcaaa tacaatgata aatgatgtta aatctaatct aacaatagat agtaacagca
      721 tagtagtgag aggtgaggta gttggataaa gagattaatc taataaatta cttgccacaa
      781 attctacaag ataaagaaga atatataaaa gtatttaatg tagaaaataa agaaataaaa
      841 acactacatg ataaattaaa ggacctatca aatgaccagt ttttagagga cctaactata
      901 agtggtataa aaagatggga aaagataatg tctataactc ctaaaagtaa tgagagttta
      961 gaagatagaa ggtttaggat ttttagtaaa tatataagta aattacctta ctcagagaga
     1021 tttttaagga actggctaga taatgtagtt ggagaaggca attatgaatt aactattaat
     1081 aatgctactt ataacataca ccttgagagt gatgctagaa atcaagattg gtttgaggaa
     1141 gttcattctt ttgtaagtaa tattaagcca tgtaatatga ctttagatta cactagagtg
     1201 cttataagca aagacaatta tatgaatttt ggtataacaa ccctaatggg tcaagaaata
     1261 actatatacc cttggagtcc accagatata gaaacttatg gagaaattga tgtattaact
     1321 ggcaatggag ttggatacca agagataaca atattttagg aggtgatata ttggctatag
     1381 ataaaagtta ttacactata attacagatg taggaaaagc aaagatagca aatgcaagtg
     1441 tcacaggtaa taaagtaggg tttgtaaaaa ttcaacttgg tgatggtgga ggaagtgagt
     1501 atactcctac tgaaagtcag acagaactta aaaatgtggt atgggaaggc aacatcggaa
     1561 atacaaccac agatgaaact gcgccaaact gcataatatt agagagttta ataccatcaa
     1621 gtgtaggtgg atttatgata agagaaatag gatatttaga tgatgaaaat aatctaattg
     1681 caatctctaa atataaagaa tgttataaac cttctataga acaaggagca gttgttgata
     1741 tgaaggttaa aactgtgctt attgtatcta atataaataa tatagaactt aaaattgacc
     1801 ctacaataat ttttgctacg ctcaaagata tacaggattt agatgctaaa ataagcaatg
     1861 taaacactaa aattgataca actaaaacag agttaacaag caacatagaa actgctaaga
     1921 cagagttaaa cactagaatt gacacagaaa atgagaaaca aaatattaaa attgaccagc
     1981 taatagcagg tggttcaaat gttgcatcta ctcaaacaat aacaattgat gattggatag
     2041 ataatcaaga aggtggattc aaagcaactg taacacatgg tttattaaca cagagaataa
     2101 ctgtaagtat tatagatgct actacaaaag ataatgtagt acccgatttc acaattatag
     2161 acgataattc tattgaggtt agaagtggag taaaagttga gttaaatgtt tatgtgatta
     2221 atgggaatgc agagactcat tttattaatg cgactgtaga tgataataga gtgtctgaaa
     2281 tgactactta ttcatcaaaa aagatacatg aagaaattgg taaggtagca gagcaactga
     2341 caggaattaa tagtaatatt atatcgactg taaataacga catactacct atatagaaag
     2401 gagcgtgaaa aaatggcgtt atcaatgagt tattttaatt tacctgacaa aagaaaatat
     2461 actaaaaatc ttgcatttaa tccatttgca ggtggaaggc aaaattttga gtggactggt
     2521 ggtgaccatg gactaaatgg tgagtttaaa gaaacttgct taagttgcac ttacaatggt
     2581 tcaacattaa attggggttc aggaaatgtt tgggtcttgg gggaatatgg tcaatataca
     2641 tttacttata attgtgaatc aatgcatgtg gatacacaac aaaaatttcc gtatacttct
     2701 aatagaataa taactataaa aggtagacct gtaatatctg gttcagatac ttcacttggg
     2761 aataaaagaa aaggatttag tgtagatttt actgtaagtg atgatactcc aaatgttaat
     2821 ttaatagtac gtgcttatct tgatgataaa ttaatacaaa acattactcc tgttgtacaa
     2881 aattcaactt taacagcaac agtaacagat agtcaactaa actctttatc cgtagatgga
     2941 aatcacaaac taaaaataca attaaatgat ggatatgata attttgatag aatttttact
     3001 tttaaaaaaa tagaaaaggg aattgatata tcaacatcat tagtaactga tagccaagct
     3061 aagtttactg taacaaaaat atacagtgaa ttgacaaaaa tagagtgtta tttagatgaa
     3121 acattaaaag agacttttac tacagacttg tattcagaaa agactataaa ctatgaactt
     3181 atagacaatg caatacacac attaaaaata gttgtaactg atgcagaaaa tgttgtagaa
     3241 gaaaaagtta taagtataag taagaatata atgccattgc aacctgatgc aacattacaa
     3301 gatatatcaa ctaagttaac agagattggg caaggcgtta gaaatggtaa aacaagcatt
     3361 ataaatactt tagcattaaa gaatatagat gcaagtttaa ataatacact cgttgagtta
     3421 tcagagaaaa ttaagggagg ttttgatagt ggagacgcta gtttacaaga tttgatgaat
     3481 caattaacac aagctaataa tactatatcg cagttaaata ctaagtataa agttgctagt
     3541 ggtacagtta cttcttttgc ggatagtact aaaattgctt atccatatct aaccgacaat
     3601 gttactaaac ctggttcttg gattaaagtt agtaatttag gctttaaacc taatattttc
     3661 tttgctgatt ttgattacta tgatgctgaa tataaaaata attataaact tttccttttc
     3721 gcttgtaatg gtgttgccac acaaagaggt gttgattttt caagtgttac atcttttatc
     3781 agaaaaagtg gtgatgagta ttttcatgct aatggatggc tatatagtaa ctcagagggg
     3841 gatgtctatt ttaataatac tggcgttcag attccagctt ataactttga ttcaactcaa
     3901 aaacatactt ataaatggta tgctattaaa tttatttaaa aggaggaaaa aacatgaatg
     3961 ttccaaaccg aataatatat gaccaaacag gtagaacaat ctttgaaaca ggcgaatcct
     4021 gtggagatgt attgccacat tatacaataa cggaattgca ttatatagat attgaatatg
     4081 gaagtataga ttatacaagg aatagagtta taggtataaa tatagaaaca aaagaaccga
     4141 ttttagaaga aataccagta tatataacag atgaggaaaa gagaatacaa gagttagaaa
     4201 atcaattatt aattgcagaa aatgagaaag taggaggatt attataatga atataaataa
     4261 tgttgtagta agaatattag cagaaagaat attaagtaag ggattaaatc ctctaaaaaa
     4321 tagagaattt cagttagatg acgtgactaa cacagagtac agaaaagctg tagaggatta
     4381 tattataaaa aatagtggag tagtagaagg agcagaacca actatataga gggttctttt
     4441 ttta
//
LOCUS       DAGQWR010000229         4429 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.233, whole genome shotgun sequence.
ACCESSION   DAGQWR010000229 DAGQWR010000000
VERSION     DAGQWR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.233"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(323..1750)
                     /locus_tag="MIW95_003741"
     CDS             complement(323..1750)
                     /locus_tag="MIW95_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HBY3153033.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSINKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKLVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTIAKNNAIVLVGNDSNKSV
                     LKSTIKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKIYN"
     gene            1941..2762
                     /locus_tag="MIW95_003742"
     CDS             1941..2762
                     /locus_tag="MIW95_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBY3153034.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETK
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKSLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            2887..4404
                     /locus_tag="MIW95_003743"
     CDS             2887..4404
                     /locus_tag="MIW95_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY3153035.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKACDLDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAIYYKSKEQKLDSFELMVHLKLHEDQ"
BASE COUNT         1721 a          618 c          607 g         1483 t
ORIGIN      
        1 tagttttaga cataacaaaa ccctcctagt tttagacagt tttacatatt tcaactgtct
       61 cctaagaggg gtatagttca tgcgagagtg gctttttgct tttttgaata gtttactgat
      121 taagtaaact accacactag ctgttaaact agctaaaaca ttaaacaaaa agttatccat
      181 gaactcacct cccttcgaat cgttgggagg ataatctttt gtacatgaac tccactctat
      241 agattgtaag ttaaatcttc ttgctaaaaa tattataaca tattatggta aattatataa
      301 ttattgttta aaagttctaa ttttaattat aaattttccc agtttctata tctataaaaa
      361 accaaccaaa agtaccatta ctattttttg aattgatttt aacagcatag acattaccac
      421 tatatatatc atctctaaaa tctatcatat caactactaa gttattgata ctgactttat
      481 gtctcttagc acattctttt aatgctatct gttttgcttg ttcctctgtt atacttcctt
      541 tatttttata atcatctgga ttacatactt tcatattacc attaacatca tatgtataaa
      601 ctttgctcat atcattttta gctacacagt atctaacatc accctctgca ccctgtttaa
      661 tgtaagcaaa tacatagtac tcttttcctg aaaaatcaaa atttttatct agtagaaata
      721 ttaaatcttc atcttctgaa taattaggtt cgtaataaaa tttcttcaca cctaatagtt
      781 ttttcactgc attagcagca tcttctactg tatattctct tccagtatta gaatcgccta
      841 tattttttgt tacatttaaa cactgctgta atatactatc actcaaattt cctatagcag
      901 ttaatttaat tgtacttttc aatacacttt tattactatc attaccaact aacacaatag
      961 cattattttt agcaatggta gaaccaacaa gagcatacac taaatcagta ccacttgcta
     1021 tataaaattc ttttactcca ctataaaatt tatttattat ctttttgtta gtatcatatc
     1081 tatctgtccc acctaatcta gttgaattag tatccttaac tagtttgtca ttcattgatg
     1141 aagtaccacc tattacataa ctttctacac cagttgtatt aaatggaaca ctttttccat
     1201 cagttaatac tatagctgct ttatctctaa ctgctacagg tgcagcactc attgcatcag
     1261 gctctccttt aaaagcatta gtcaatatga ctttatttac tttgttaatt gaatttattt
     1321 cttttgcaac attatagctt gtcttaattc tatcactacc ttgaagtctt ttaatctcta
     1381 ttcctttgcc ttttagaaca gtttcagtat atttattaat agagttttct cctccaatta
     1441 tatatacttt ttttgccttt tctaatcttt taagtgtagc atttggtatg ttatttttct
     1501 tagtcaaaag aattggagcg tttatagctc ctgcaagtcc acttgcactt aatccatcag
     1561 ccattgtact gtctgcatta attaatatag ctgtagtata gttttgtttg tctgcaatta
     1621 taccagcagt ttcgtattta tctgcacctt taattgattc aattttatca agagcatttg
     1681 cagatatagg gcaactagca ataaatatag ataaagataa ccctattgtt aataattttt
     1741 taattttcat aatatctcct tctcttaaat attttcacct aaattatata atacttttca
     1801 ggaaaatgtt accataaatt ttaaaaatta ttattactta atataatatt aatgaaaaat
     1861 atcattctta aattaatgac tttggtagaa tatggtgata aaataaaatt agtgagagac
     1921 aatataaggg ggattatatt atgataatca gtaaaaaaat aacagcaata ttagtatcat
     1981 ttttaattat gatatcaatg gttggatgtg ctccaaaaga aagtgctaca gaagtagcta
     2041 aaaagtacct tgatagtata gaaatcaaag actacgaagt agcatataat ttattaagta
     2101 aagacagtaa ggaaagtgtc agtctagaaa aatttgaaga atatcaagat actttattta
     2161 aacttcaaac cctaaaagat tataaacttg gtgaagaaaa taaagtagag aaatacaaat
     2221 attctaataa agagtacaca aatcttgtta aaataaaaga aactgatact ataaagtata
     2281 tcactgaaaa taaagaagat actgcaaatt ttgatagata ttttataatc gaagataatg
     2341 aatataaatt attatggcat aaagacttta aaagagctct tagctctaat tatgtcaaaa
     2401 tcgctaggtc agaaacaaaa aaagatgaag ataaggatta taataaagct gctattgata
     2461 tcaaaaaagc tatagaaatt gatgataaaa atccacttgc atattataca caatcatata
     2521 tttatataga gttacaacga tatgatgagg ctttaaagtc aattgataaa tccttagaat
     2581 atctagataa aaataatgaa gcttataaaa tagacgctag tgatgcttac aatttaagag
     2641 gtgttatttt tatgagtaaa tcagaattta ataaagctag agaatcttac aataaggcgc
     2701 ttgaacttaa cccggataat gaatatgcaa aaactaatct aaatgatatt aaaggattat
     2761 aaggcattaa tattatagaa aataagtatg ttgaaagtta aattaacttt tgacatgctt
     2821 attttctata ttttaaactc tattgattta ctatataata gtgcataagg agtgtgatta
     2881 cttattatga gtgttgccat atacttaaga aaatcccgtg ctgatgaaga ggcagaaaaa
     2941 caaggcgaat ttgaaacttt aagtagacat aaatcaaccc tactaaaact agcaaaagaa
     3001 caaaacttag atgtaattga gataaaagaa gaattagttt ctggtgaaag tataatacat
     3061 agacccaaaa tgcttgaact tctaaaagaa gttgaagaaa ataaatatga tgcagtctta
     3121 gtaatggact tagacagact tggtcgtgga gacatgaaag accaaggtat catattagaa
     3181 acattcaaag aaagtaaaac caaaatcatc acacctagaa aaacatacga cctaacagat
     3241 gaatttgatg aggagtattc agaatttgaa gcatttatgg caagaaaaga attaaaactt
     3301 atatcaagaa gaatgcaaag aggtagaata aaatcagttg aagaagggaa tttcatagga
     3361 acttctgctc catttggata tgatgctgta acaacaggta gaaaagaaag aatacttgta
     3421 ccaaataagg atgctgatgt agttaggact atctttgact tatatattaa tgaagatatg
     3481 ggttgtagta aaatatcaaa atacctaaat aacttaggca ttaaaactgc tacaggtgct
     3541 aattggtaca attctgcaat tacaaatata ataaaaaata aggtatattg tggctacatt
     3601 cagtggcaaa aaaaggacta taaaaaatct aaaaatccaa ataaaataaa aactgttaag
     3661 ctaagaccta aagatgaatg gattgaagca aaaggaaagc atgaaccttt aataagtgaa
     3721 ataacatgga aaaaagctca aaatatactt aaaaaaaacg gtcatgtcag ctatggtaat
     3781 caaataaaaa atccacttgc aggaatagtg atttgcaaaa attgtggtag accattagta
     3841 tatagaccat atgcagacca cgactatata atttgttacc atcctggttg caataaaagt
     3901 tctcgatttg aatttattga agctgccatt ttaaaatctt tggaagacac ggtaaaaaaa
     3961 tatcaattaa aagcatgtga tttagactta gataaaaata ataaagatag taatatagaa
     4021 tttcaaaaac gagtcttaaa gggcttagaa actgaattaa aagaattagg taaacaaaaa
     4081 aataagttat atgatttatt agaacgtgga atatatgatg aagatacatt tattgaaagg
     4141 tccaacaata taagttcaag aactgaagaa attaaagatt caattaagac agtaaaaaat
     4201 agattgagta ctgttaaaaa agacaattct aaaataatag aagatattaa aactgtgtta
     4261 tcactttatc atgattcaga ttcccttgga aaaaataaac tcctaaaatc tgtaattgat
     4321 aaagcaattt attataagtc taaagaacaa aaactagatt cttttgaatt aatggttcat
     4381 ttgaaattac atgaagacca gtaataccaa taacatataa agagtagtg
//
LOCUS       DAGQWR010000230         4419 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.234, whole genome shotgun sequence.
ACCESSION   DAGQWR010000230 DAGQWR010000000
VERSION     DAGQWR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.234"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(54..1016)
                     /locus_tag="MIW95_003744"
     CDS             complement(54..1016)
                     /locus_tag="MIW95_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBY3153036.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHIFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVLSPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(1186..2376)
                     /locus_tag="MIW95_003745"
     CDS             complement(1186..2376)
                     /locus_tag="MIW95_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY3153037.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            2682..3128
                     /locus_tag="MIW95_003746"
     CDS             2682..3128
                     /locus_tag="MIW95_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBY3153038.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEG"
     gene            3130..4224
                     /locus_tag="MIW95_003747"
     CDS             3130..4224
                     /locus_tag="MIW95_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBY3153039.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            4312..>4419
                     /locus_tag="MIW95_003748"
     CDS             4312..>4419
                     /locus_tag="MIW95_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBY3153040.1"
                     /translation="MKSLVLYSSLTGNTKKIAYAIYDEIQEEKDIKDVNE"
BASE COUNT         1719 a          547 c          637 g         1516 t
ORIGIN      
        1 cgtttaaatc taataaattt tgtcttatgt ataacttgat agattatcaa ttactactcc
       61 ttatacaagg cttcaacgtg tttattatgc atttgagtaa ttatcgtagg tataatagaa
      121 attattgaac caattactat caataaaatt cctataacca tgtttatagg gataatttct
      181 ttaagtatta ataatgctag tattggagat agtactggtt taaagaagaa tactaaagat
      241 gtaagactag tagatgtagc ttccattgcc ataaagtaaa gtgcataacc aacacctgta
      301 acaaatacga atacataaat cataatgggc atattgtgta aggtatatcc actaaaaata
      361 gggatatttg caaatatatg aagtttattt ttatttaata tatctgcgat aaaattaatt
      421 ttagtgagta aaaccaatat caacatttcc aaactaccaa atataaagct aaagcaagtt
      481 acaactattc caccaaattt taaggttttt cttttaccaa atacagtata cattgaaaaa
      541 ataatagcag aagataatgt taagaatatt ccactaagag ttagttttgt atgcaatgga
      601 tttattataa ctataattcc aagaacttct aatataagtg aaattacatt gtgcctatat
      661 attttttcct tcaaaattaa gtaagctaaa agcattacaa agactggatt acaactgaac
      721 aaaacagcaa ctacagatgc ctttgtgttt agaacagcca gttgatacaa tgacatactt
      781 actactacac acataaaacc taaaaatgca aagtatttta agtctgattt tgtaatagaa
      841 agactaagag ctcttaatct ttttacagca aaaggtagta agactaaacc gccaacaaaa
      901 aatcttgata aagttatttg taagggatta aagtcattag tgacagtttt tagactaatt
      961 tccattgaac taaaaaataa ggtagtcagt attatataaa cataaccttt tttcaagtca
     1021 aacacctcct tgtaatttat attagtacta tagataagaa tattcaagca tattggaata
     1081 atatggtatg tataaaatat agtacataaa attataaact accttaatat agaatgtaag
     1141 tattctatac taaggtagtt aattatttga attactttga tttagttatt ttaaattata
     1201 taatttggct aatttttcaa atgctggtaa agaactttta actttatcat aagatacaca
     1261 atatgatact ctaaaatgac ctggacaacc aaaagaagaa cctggtacta ataataaatt
     1321 aaattgtttt gcatcattgc agaatttttt atcatcttca attggagctt ttgggaacaa
     1381 atagaatgta ccattaggtt taacacaaga atatcccata tcgattaaac tattatatag
     1441 aaggtcaaaa ttctttttat aaatatttac atcaacttct gcatctaaac ttcttgcaat
     1501 aaccctttga aaaagagaag gagcatttac aaatccaagt attctatttg caacatttaa
     1561 tgaagacata acatcatcaa aatcatccat ttgcccatta gcaacaatat atcctatacg
     1621 ttcaccaggt agagataatg atttactata agagtaccct ataaatgaat ttctatagta
     1681 ttttagtaca caaggaactt tggcaccatc ataaattatt tctctatatg gctcatcaga
     1741 aattagatat atgcttgtat taaattcttt ttgttttgaa tccaataatt ctccaagatt
     1801 ttttaataat tcttcagaat atatgacacc agtaggatta tttggagtgt ttattattaa
     1861 agctcttgtt tttggagtaa tagcattttt tagagcatct aaatctggtt caaaagtctc
     1921 tatatttgta ggaacttcta ttaattttcc atcatagttt tctgtataat ttttatactc
     1981 accaaaatac ggtgcaaaag caataacttc atcaccagga tttaaaagag tttttaaaat
     2041 aatatttaga ccaccagcag caccacaagt cataattaaa ttttctcttg taagatttaa
     2101 tccatctttt ttatttatat gttcagcaat agcatctctt acatcctcat atcctgagtt
     2161 gttcatatat ccatgaacta aatttggaga ttcttcattt aaaatatcat ttataactgc
     2221 tttaatagtc tctggaggtt caacacttgg atttcctata ctaaaatcaa aaacattttc
     2281 ctcaccataa atatcagaaa gtttctttcc ttcttcaaac atagctctta taacagaact
     2341 atttgcaact aaagtttgca ttttattaga tatcatatgt aacactcctt tttatagatt
     2401 tatattgctt atgtatatat taccatattt ttactgaatt tagacaattt tcgaaaaatt
     2461 acttgtttga aaatataaac ttttatataa aatatattta tgtacagtgc atattaaaaa
     2521 ttccaatttc taattttatc atgattattt tatataatag ttttttaaag aattaataaa
     2581 acatagattt agagaataaa atattcttga ctatattaat tgtttaatat aatatagttg
     2641 atatagtcaa acatattata attatagaag ggaaatttaa gatggatttt aaaaatttac
     2701 agtatgaaag tttgtcatat attatttgta acttgcaaaa aaactttaaa ctttattgtg
     2761 aaaaatgttt aaaaccatat aaacttacga atggtctata tttctattta atttatatta
     2821 ataaaaatag gaattgttct ttgaatgatg tttctacaga atttgaagtt gataaagctc
     2881 atacaacaag aactatatct aggttagaac aagatggata catagaaaaa atccaaaacc
     2941 ctaatgatag tagagctttt cagttgagag taacagataa gggtgaggaa gtattagggg
     3001 atataaaaaa catattttca aagtgggata accacataaa aaaagagttc tcagatacag
     3061 aatataaaga actggtaaag aatttacatg tggtaaagga tataaaaaca gctgtggagg
     3121 aaggatagga tgtattgtaa tttattaaaa ccaattaaaa ttggaaattt ggaattaaaa
     3181 aatagagtat cttttgcacc tacaagcatg ggtcttaaat tggaagaaaa aattaaaaag
     3241 ttttctgata ttgctaagtc aggagtagct ctgattactc taggagatgt atctattaga
     3301 cctagttttc ataaggtagc tataagccta tcggatgaag atggagtcat taaatacaag
     3361 aaaattgtag atgaaataca caattctggt gcaaaggtat cagcacaact attttgttca
     3421 gattatgatg ttaatttaat taaagatact atgaagatgg gaattacttc acatgatgag
     3481 ataaaaaaaa taatgaatga cggagtaaaa gattacataa caaatatgcc caaagaagaa
     3541 attaaaaata ttataaattt atttaaagta acagcattga atgcaaaaaa agcaggtttt
     3601 gacatgattc aaattcatgg tgataggctt gtaggaagtt tttcatcaag tatatttaat
     3661 aatagaaatg atgaatatgg tggaacgtgt gataatagaa gtagatttgc aagcgaaata
     3721 attagtagta tccgagatga agttaaggac attccaatag actataaatt tgcaataaga
     3781 caagaaaatc ctcattatgg aaatgcagga gttttacttt cggaagttga gtattttgtt
     3841 aaaaaatttg aatccctagg tgtaaacagt tttcatgtaa cacttgcaaa tcattcaaaa
     3901 ttagaagata ctataccaac caataatcat ccttacttta aagatgaagg ctgtttttta
     3961 tatttagctg atgaagtaaa gaaacacaca aatcttccag tatgtggggt tggaaaattg
     4021 tctagcccag attttataga aagtattatt tcaaataata gagtagatat ggtttctatg
     4081 tcaagacaat tactagcaga tagtaattgg ttacagaaag taaaagatgg aagagtagat
     4141 gagattaaaa agtgctgtta ttgtaataag aagtgtgcag atgctttaca aacacgctct
     4201 cagtttggat gtatactaga ttagaatatc tttataaaaa taaataattt gttaaggtta
     4261 atatattttt ggtgatttag ataagtattt agataaagag gaggagacat catgaaaagt
     4321 ttagttttat attcaagttt aactggaaat actaaaaaga ttgcttatgc tatatatgat
     4381 gaaattcaag aagaaaaaga cattaaagat gtaaatgaa
//
LOCUS       DAGQWR010000231         4118 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.235, whole genome shotgun sequence.
ACCESSION   DAGQWR010000231 DAGQWR010000000
VERSION     DAGQWR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.235"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1874)
                     /locus_tag="MIW95_003749"
     CDS             complement(<1..1874)
                     /locus_tag="MIW95_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3153041.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVKTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKMFFKDFEIEKINLYIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKFKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKIIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     "
     gene            complement(1861..2628)
                     /locus_tag="MIW95_003750"
     CDS             complement(1861..2628)
                     /locus_tag="MIW95_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3153042.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALKGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTMDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            2916..3590
                     /locus_tag="MIW95_003751"
     CDS             2916..3590
                     /locus_tag="MIW95_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3153043.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            3614..>4118
                     /locus_tag="MIW95_003752"
     CDS             3614..>4118
                     /locus_tag="MIW95_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY3153044.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLINEKISDDIDSEI
                     FRIENSVE"
BASE COUNT         1539 a          547 c          432 g         1600 t
ORIGIN      
        1 aaataaatga ctagagttat gataattggt acaagtaatg accttggtat aaacttttgt
       61 gctatagaca tagcaaataa attatgaact gtacctacaa ctaatggcat taaaaatata
      121 atttttaact gttttgatat tattttctca atatctttat tatttgctcc aagttttatt
      181 aacataatat atctttcctt atcatcatat atatttgata attgtttaaa taagattata
      241 ctacctgtac ataaaagaaa tactacagac aaaaatatgc ctataaacag cattagacca
      301 cttaatttta tcaagttatt ataactttca atacttgatg taaaattaaa aggataagta
      361 aatttcatat tttcacttac tatattttta aattttaaag tcaaatccag ttcttttttt
      421 tcatttttta tatcaattaa tcttattaat ctagaatttc catgtaattt taactgatta
      481 aatacgtcat cttttactac aattaaatca aacgtagctt gtgaatttat ttctggctcc
      541 aagatgcttt tttgtacctt aaacagattt atatcatact catttgtatc tttaatatac
      601 aagtttattt tttcaatctc aaagtcttta aaaaacattt ttttgtatga atcagaaaca
      661 tagtacacag attcctttga agttaattct tcataattgt tattctgata ttgtcttaat
      721 tttttaaaat cactttctct aataatatct acagaagttc tatcttctat ataactattt
      781 ccatttttat aatacatttc aatatttata tctttgtcta taagttctat tgttttatca
      841 aagattatct tattatcttt tttatattta tataataatt tgtcaatttc gttatttaca
      901 taagaatttc ctaaatttat ggtataactg tatttataat tttcacttat gttcctatct
      961 atatcgtaat aaagtgatat ggtaaaccct agcatagtta cactcgtagc tattaatatt
     1021 gctattacag ccaatacact actattgctc tttaatttaa atccaagttc agaaaatgca
     1081 attaaattct gtcccttata ataaaaactt tttctcttct taacagcatc aataatcata
     1141 tacatagttg atgaaaaaaa taaaaaagtt ccaggtatta ttgtaactaa tataaaaact
     1201 gaaagcataa tattttcagc aataaaataa ctaattgaca ttaaataaga aatagctatt
     1261 aataaaacac caagtacaca ttttaacttt ataaattttt taaacttata cttttctgga
     1321 tatttattaa ataactctat aattctctta tttctgataa ctatagtatt tcttattcca
     1381 ataactataa atattattaa aaataccgct agtgtcttaa ctaaggcctt tatacttaag
     1441 ttcatattta ctactatcat ttcattcatt aattttacaa gcatcatagt cataagcttt
     1501 gaaaataaca gtcctaaaaa tatacctatt agtattgcaa ctatacccat caaaaaagtt
     1561 tcatataaaa atatttttcc tatctgattt ttcctcattc caagtaaact ataagttcct
     1621 acttctcgcc tacgtctatt gaaaaaaaat gagtttgaat aatatataaa taaaaataca
     1681 aataccacta ttacaattga acttgtatta atactcatcc tagttttttc tccaagtgaa
     1741 ctcaacgctt cattatactc tatagattta aaactaaaaa atacaaatac actaaataca
     1801 attgaaataa agtacattaa ataattaaaa aaactattct ttacattttg cattgcaatt
     1861 ctaaataaag tcatttttat taactcctcc aattaaggaa actgtattta ctattttatt
     1921 atagaactct ctgttacttt ctccctttac aatttctgtg aatattattc catcctttat
     1981 aaagagtatt ctattgcaaa aactcgcact tatagcatca tgagttacca tcaatattgt
     2041 tttattgctg tctttgttta attttaatag acaattcaac aaatccctag cagatttaga
     2101 atctaatgct cctgtaggct catctgcaag tactaatgat ggttttgttg ctatagctct
     2161 acatacagct cctctttgct tttgtccacc agaaacttcg ccaggatatt tatctaatat
     2221 agattctata tttaattctt ttgaaatctc ttttattcta ttttctatta ctgatactct
     2281 ttcatttttt aaagaaagag gcaacattat attttctttt attgtaagtg tatccaaaag
     2341 attagaatct tgaaatacaa agcctagatt atcacgtcta aaatctgcca attcattttg
     2401 cttcatttta atagtattct ttccatttat ataaatatct cctgatgaag gtaaatccat
     2461 tgtagaaata atatttaaaa gtgttgattt accagcaccg gaagacccca taataccaac
     2521 aaattctcct ttataaacct tgaaagttat tccttttaga gcttcatgtt ttgaaccttt
     2581 tcttccatac tcctttttta tattttcaac agataatatt tcttccatag ttaataatca
     2641 cctttcatca caattagtta tttatcaatt ctcacaaata tattatatat attaaaatgg
     2701 cttttaaaca tttaattata ttaatttaag gttacatttt cgtaagtatt aattatattt
     2761 cttcaaattt attgcaatta tgtttaaaat ataatttatc aagtaaaata tagttatatt
     2821 aatataatta ttttaggtaa taaggtagaa atattttaat ttatcaagta gaatatagtt
     2881 atgttaatat aactattttg aattaggagg taagaatgtt taaaataatg gtagttgaag
     2941 atgacgtctc attgaaaaat atcatagcaa agtgtcttac taaatgggga catgatgttc
     3001 atcaaataga aaatcttgaa aatataatag aagagttcaa aaactataac ccagaattag
     3061 ttttattgga cataaacctt ccattttatg atggttttca ttggtgtaat gaaataagaa
     3121 aaatttcaaa agttcctata atttttattt cttcaagaaa ttctaatatg gatgtaataa
     3181 tgggtgtaaa tttaggtgca gatgattata tacaaaaacc tttttctgta gatgtcttag
     3241 tagctaaagt taatgccctt ttaagaagaa cttataattt tgttgataat aactcaaatc
     3301 agattattca caatggtgtt actttagatt tatcaactgc aacaataaac tatgaagata
     3361 atactattga gctaacaaaa aatgaaatca aaattcttca tgagcttatg aaatataagg
     3421 ggcagatagt aagtaggaat aaactcatga aaaaattatg ggataatgat tggtttgtag
     3481 atgataatac attaactgtc aatgtaaata gaatacgctc aaaattaaat gaaattggtt
     3541 tagaagattt tatagaaaca aaaagaggat tgggttatat aatcagttaa aacaattcta
     3601 agtaggtgaa tttatgagct ttaaagattt tctcaaggat aaactaggtt ttttagtata
     3661 caattttact ttcttaattt ttacagtatc agttattata ttttctcctg tagatgtttt
     3721 tctgactgat actattttat atatattagt tgtaaatgtt gtgtttttat ttttatatct
     3781 atttattagc tacatcaaga agaacaaatt tttagataag ataaaaaatg atacattcaa
     3841 aattaatatg aatgatatag aattagctag atgtaaaaat gaagaaaaaa tatacttgaa
     3901 cattattaaa aatcatcaat atcaatgtaa taacataata aataataata tcgagaaatt
     3961 tgaagaaaac aaagagattt taaatatgtg ggtacacgat gtaaagatac ctatagctat
     4021 aataaaactc attcttgaac aaaatgaaaa tttaattaat gaaaaaattt cagatgacat
     4081 agatagtgaa atatttagaa ttgaaaactc cgttgaaa
//
LOCUS       DAGQWR010000232         4006 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.236, whole genome shotgun sequence.
ACCESSION   DAGQWR010000232 DAGQWR010000000
VERSION     DAGQWR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.236"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..820)
                     /locus_tag="MIW95_003753"
     CDS             complement(<1..820)
                     /locus_tag="MIW95_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY3153045.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHIIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNVIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYN"
     gene            1008..1913
                     /locus_tag="MIW95_003754"
     CDS             1008..1913
                     /locus_tag="MIW95_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBY3153046.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            1922..3106
                     /locus_tag="MIW95_003755"
     CDS             1922..3106
                     /locus_tag="MIW95_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY3153047.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     RIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNTYINE
                     YI"
     gene            3221..>4006
                     /locus_tag="MIW95_003756"
     CDS             3221..>4006
                     /locus_tag="MIW95_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBY3153048.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIM"
BASE COUNT         1591 a          367 c          613 g         1435 t
ORIGIN      
        1 aattatattt tgtaaatata atttctccat cactataaaa aacaatatct cctattctat
       61 ctgttctata aattgataca tggttttgtt ttagattatt aataacctcc ctatgtggat
      121 gaccatattg atttttatat ccacatgata ttgcagctat atctggacta atttttttta
      181 taaattctaa agatgaagaa gaattacttc catgatgacc tatttttata aaattaatat
      241 tatttaattt aaaagaatgt aaaatttctt tttcattttc ttcttcagca tcacccataa
      301 ataaaaaaga attatcgtta aaagttaatt tgaaaactat agaatttaaa ttagattctt
      361 cttgtatata tgatggactt aatacatata tatttatatt gttatctatg tttaaaacat
      421 catttttata gagatgctga atagataagt ttttatccgt acaagaattt attagatttt
      481 gataagcctc actatcagtt gattgttcag gcatatatat ggagtttaca ttaaattttt
      541 ttataacatt atcaagactt cctatgtggt ctgaatctgg atgagttgct attataatat
      601 caatagtttt tattcttttt tgtctaagat aactaataat tatattttcg ctatcttcat
      661 cacctccatc aattaatata ttcttatttg tgggtgtttg aactaaaata ctatcacctt
      721 gtccaacatc aattatatgt atagatagta gagatttttt gtcacagccg attaaaagtg
      781 atattataat aagtattgat aaaaaaattt tatttctcaa taaaaaatca ctccattcat
      841 tacatgttta tataaattta tgatgggtaa tataagatat atatgactta ttatatttta
      901 tattttaaac aatataaatg aaattaatac cataataaat ttggagtttt ttatatatac
      961 aaaataaaat ttttaaaatt aagcaataaa aaaggtagga ggattttatg tttttcaaag
     1021 atattgaact aaattcaaaa aaagagttag acccatattt tgatttagta gattatgaag
     1081 cttgtgagta ctgttttagt actttatata tgtggcaaca tgtttataag acaggatatt
     1141 atataggtga agattttgcc gttttagttg gggaatacga aggagatagt ttctcaatat
     1201 taccacttgc taaaaaagat aaacttccag aagttgtaga ttttgtcctt gaatattttt
     1261 ctaaaaataa taaaaaaata tacttaagag gtattactac tgaagtagta gagtttttaa
     1321 aagaaaaata tccaggtaga tttgagtata tagaagaaag agatttattt gactatattt
     1381 atgatgcaga aagtttaaga actctagctg gtaaaaagaa tcaaaagaag agaaaccata
     1441 taaactattt cttaaaagag tatgcaggta gatatgaagc taaattatta gataaagaaa
     1501 actttgacga gtgtttagta ctaatgaagg aatgggaaag taataaggaa gaaaataatg
     1561 aatttgatga aagtatggat gacgaattaa taggtatcaa aaagatattt aaccattatg
     1621 acatattaaa agataaggta aaagtgtttg gtgtttatgt ggatggaaaa ttagaggctt
     1681 ttagtatagg agaactttta aatccaaata tggcattgat tcatatagaa aaagctaatc
     1741 ccgatataag aggtctgtat ccatttataa atcaacaatt tttggtaagt gaatttaagg
     1801 acgttgagtt tgtaaataga gaagaagatt taggtattga aggattaaga aaagctaaac
     1861 tttcttatca tccttgcaga tttgttgaaa agtacagtgt tagggaggct taatagacta
     1921 tatggagatt aggtatgcta aagaagaaga gatagaaagt ataaaagaga tttggagtta
     1981 ttgttttaac gatacggaga gttttatgaa gtactatttt aatgataaat ataaatctga
     2041 aaatactgta gtagctttag atgaaggaaa aattatttct tctcttcagc taaatcagta
     2101 taagttatta ctaaatagca aggtatataa tacatcatat gtagtaggag tttctacact
     2161 tccagaggga agaggagctg gatatatgaa caaagtaatg aaatttactt taaacgaatt
     2221 gtataaaaaa gggcaattag tttctatatt gatgcctatt gactatagac tttatagaag
     2281 atttggatat gagcattgtt acgaccagat tgaatatact attaacacag atgatttaaa
     2341 aaattttaag tcaagtggaa aaatgataaa gtctaattta tcacaaattg atgatttgat
     2401 acgaatagat agaacttttt taaatgaggt taatggtaat gtgttaaaag atgaacatta
     2461 ttatgaaaac ttatttaaag agattcaaag tgaagatgga tttttataca ttcatgaagg
     2521 aaatgaaaaa gatggatata tagtttattt tcttcaggaa gataaaatgt ttgtcagaga
     2581 gttattttat aaaaatatag atgcccttaa atcaatgctt aaatttatat ataatcacaa
     2641 tactcaatgt aagatagtta ctatatctac tcctacaata gataagataa gatttatatt
     2701 agacaaccct aaagatagtg atataaaaat aaaacctttt atgatgggaa gagttataaa
     2761 tgtaaagaag tttattgaag atatagatat tgagaaggat attaatagct catttaattt
     2821 attaatagaa gacaagttta tagatgaaaa taatggtcta tttaaaattt ctatacagaa
     2881 taagaaggta tctgtagagc agttagataa aaaaggtgca gaaaagcctc aagaagattt
     2941 tgatattaaa ttagatataa ataccttaac acagttatca ttttcttata tagatgtaaa
     3001 tgaagctatt ttcttaaatg atataaaaga tgtttcagaa gaaacactag aaacacttaa
     3061 ttgtattttt tctaaaaaaa atacctatat aaatgaatat atataggtag aatacgttaa
     3121 aagattttaa aaatcttttg acgtattttt ttgtttagat aacaatttat cttgtttaaa
     3181 aattttctat ttgaaaataa tctaaataat atacttttat atgggagtga ttataattca
     3241 aataagaaga ccattattaa ttatattaat gtttgtggta gctatatcat ttatcattac
     3301 aaataacaca aaaaacgacg aagaacttaa tgataagata ataactatac aaggggttgt
     3361 caaaggaaaa attgaaaaga aaagatacaa tcagtacaag gtaggggttt ttctaataaa
     3421 tgattataca aaaaataaaa atttgaagat tggtcagaaa gttaatatta caggtaaatt
     3481 caaaagttta gataaaatga agtatgatga ttttgattat ggaaggtata taaaaagtac
     3541 tggatataaa ggtatagtgt atataaataa ctataaaatt attgataaaa ataaacttta
     3601 ctctttaata ggggaaataa agttttatat tagtaaaact tataggtact tatataagaa
     3661 aaactcagat tttataaatt ctatactatt agcagaagtt gaaaatttga ctgaggaaca
     3721 aaaagaaatt tttactagaa ctggaactag tcatgtaata tctatatcag gtcttcatac
     3781 aggaatatta tgtgtaatta tatcctttct attaagaggt ataaataaat tgtataaatt
     3841 attaatactg tcgattttta taactttata ttgtataatg gtaggtgcgt ctccatccat
     3901 aattaggtct atagcatttg ttatgatttt ttatttatca atatttattg atagaaagaa
     3961 agatggaata tcagcattgt ctctaattgg gataatttta attatg
//
LOCUS       DAGQWR010000233         3939 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.237, whole genome shotgun sequence.
ACCESSION   DAGQWR010000233 DAGQWR010000000
VERSION     DAGQWR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.237"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..239
                     /locus_tag="MIW95_003757"
     CDS             <1..239
                     /locus_tag="MIW95_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005209661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153049.1"
                     /translation="VEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            497..1546
                     /gene="carA"
                     /locus_tag="MIW95_003758"
     CDS             497..1546
                     /gene="carA"
                     /locus_tag="MIW95_003758"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBY3153050.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            1739..>3939
                     /gene="carB"
                     /locus_tag="MIW95_003759"
     CDS             1739..>3939
                     /gene="carB"
                     /locus_tag="MIW95_003759"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBY3153051.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYL"
BASE COUNT         1630 a          400 c          777 g         1132 t
ORIGIN      
        1 gagtagaaga atctagacca aacatattag atgctataag aaataaacaa gttgatatag
       61 taataaatac accaactaaa ggaaatgact ctacaagaga tggatttaag ataagaagaa
      121 cagctattga attttctact gaaataatga catctttgga tacactaaaa gctttagtag
      181 aagttaagaa gaaacattta aataaagatg agttaaaagt ttacaatata gctgaataga
      241 aattaagttg attgtatagt ttaagaattt aattattaaa taaaaaaaaa gaggttattt
      301 tttctaggag tttttacttt tgagataact tctttttttt aagaaatttg tagtataata
      361 gaaaaagata aaaaataaat gtaaataagt ataaaactag aatgtaaaag tgaaaaatag
      421 atatagatat ttaaaagctt atatttagtt aatgttcttt agtttgtcta aagagaatta
      481 taggaggtac tgtagtatga aagcaaggtt aatattagaa gatggaacag tttttatagg
      541 aaaggcattt ggatatttag aagaaagtgt aggagaagta gtatttaata cttcgatgat
      601 aggatatggt gaagttttaa cagacccttc ttactatggt caaatagtta ctatgactta
      661 tcctcttgta ggaaattatg gtataaattt atcatcagca gagtctgaaa aagttcaagt
      721 aaagggattc atagtaaggg agaaaagtga ttctccaagt aattttagat gtgaaattga
      781 tatagaccaa tatttaaagc aaaataaagt tatcggactt gaaggaatag atacaagagc
      841 tttgactaag atacttagaa ataatggaac aatgaaaggt ataattacat tagaagacag
      901 taaattagaa gatgttaaac ataaattgga taagttttca aatacagaag cagttagaac
      961 tgttacaaga aaagaagtag agcatataaa aggaaatggt ccaaaagtgg ctgttatgga
     1021 ttttggagta aaaagaaata tattaagatc atttatagct cgtggatgtg atataacaat
     1081 atttccagct acaacatctc cagaagatgt attgagtata aaccctgact taatattctt
     1141 gtcaaatgga cctggagacc cagaagacct agaagatgtt atagaaaata taaaagctct
     1201 tattggtaaa aaacctatag ttggaatatg cttaggacat caacttttag ctttagctct
     1261 tggaggaaaa actgctaaac ttaaatttgg gcatagaggt ggaaatcatc cagttaaaga
     1321 tttagaagaa ggaaaagtat ttataacttc tcaaaatcat ggttattatg tttcagaagt
     1381 tcctgaacaa atgaaagtga cacatataaa tttaaatgat aatactgttg aaggaatgag
     1441 acatgagaag ctagatgtat acagtgttca ataccatcct gaagcttgcc caggaccaaa
     1501 agataatgac tatatttttg ataaattttt agaattagta aaataaataa cattagaata
     1561 gctaactaaa aaacaagaat aacttaaatc aaaaagtttg gtttaattaa catagatgta
     1621 ttaaatagat gtattgatga gtaaatttga acttaagata aaataaagta attaaagttg
     1681 attgtataga tgatgaagat aagaactgtg aaaattgttt agataaggag aaagagttat
     1741 gcctaaatta gatagtataa agaaaacttt agtattaggg tcagggccaa taataatagg
     1801 tcaagctgca gagtttgact attcaggaac acaggcctgc caagcattaa aagaagaggg
     1861 aattgaagtt gtattagtaa atagtaaccc agctactata atgactgata aagaaattgc
     1921 agataaaata tatatagaac cattaacaat agaatttata gaaaaaataa tagaaaaaga
     1981 aagaccagac agtttacttg caggtatggg tggtcagaca ggattaaact tagctgtaga
     2041 attacatgat gctggtatat tagataaata taatgtaaaa gttataggaa catctataga
     2101 atctataaaa aagggcgaag atagagattt atttagagaa gtaatgaaag aaataaatca
     2161 accagtaata gttagtgata tagttacaaa tttagaagca ggtcttgagt ttgccaataa
     2221 gataggatat ccagtagttg taagaccagc atatacgcta ggtggaactg gtggaggtat
     2281 agctgatact gaggaagaat taagagaaat actttcacat ggactacagc taagtcctgt
     2341 tggacaagtt cttcttgaaa agagtataaa aggttggaaa gaaatagaat atgaagtaat
     2401 gagagatgga aatggaaact gtataactgt atgtaatatg gaaaatgtag acccagttgg
     2461 agttcataca ggagacagta tagttgttgc tccaagtcaa actctaagtg atgaagagta
     2521 tcaactactt agaaaggcat caatagacat aataaatgca atagaggttc aaggtggatg
     2581 taacgttcaa atagctttaa acccacatag tttagaatat gcgataatag aaataaaccc
     2641 aagagttagt aggtcatcag ccctagcatc aaaagctaca ggatacccaa tagcaaaagt
     2701 agcagctaag atagctttag gttatacttt ggatgagata gaaaatgcag taactaaaaa
     2761 aacttatgct tgttttgagc caacattaga ttatgtggta gtaaaaatac caaagtggcc
     2821 atttgataaa tttagacatg caaatagaaa attaggaact aagatgatgg caactggtga
     2881 aatcatgagt ataggaagta actttgaggc tgctattctt aaagggataa gatcacttga
     2941 aacaggaaaa tattcattgg ttcatgctcc atcagaaggt agaacattag aagaattaaa
     3001 ggctagagta gtagtacctg atgatgagag actatttgac ttagctgaaa tgataagaag
     3061 aggctataaa gtagaaatga tatcagaaat aactggtgtt gataagtggt ttataaataa
     3121 atttaaatgg atagtagaac aagaagaaaa gttaaaagtt ctaaaaatag aagacttaga
     3181 taaagaatat ttacatgaat taaagaaaaa gggattttct gataaaggta tatctgactt
     3241 aatgaagata agcccagaaa aattatatga attgagaagt ttatataata tacaaccagt
     3301 ttataaaatg gttgatacat gtggaggaga atttgaagct ctatcacctt attactattc
     3361 aacttatgaa caatatgatg aggtagtagt tagtgataag agaaaagttg tagtattggg
     3421 ttctggtcca ataagaatag gtcaaggtat agaatttgac tattgttcag ttcactgtgt
     3481 aaaatcttta agaaaaatgg gaatagaaac tataattgta aacaataacc ctgaaactgt
     3541 aagtacggat tttgatacat ctgataagtt gtattttgaa ccattaacag aagaagaagt
     3601 tttaaatata atagaaaaag aaaaaccaga aggtgtaata ctacagtttg gtggacaaac
     3661 agctataaaa ttggctaagt ttttacatga aaaaaatata cctatactgg gaacagactt
     3721 tagagatata gatgcagcag aagatagaga aaaatttgat gatttactag agagactaga
     3781 tataaataga ccaaaaggaa aaggtgtatg gacaactaat gaaggtgttg aaattgctaa
     3841 ggagttaggt tatccagtat tagttagacc atcttatgta cttggtggtc aaggtatgga
     3901 aataacttat aacgaagaaa aactaattca gtatttgga
//
LOCUS       DAGQWR010000234         3862 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.238, whole genome shotgun sequence.
ACCESSION   DAGQWR010000234 DAGQWR010000000
VERSION     DAGQWR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.238"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            119..1609
                     /locus_tag="MIW95_003760"
     CDS             119..1609
                     /locus_tag="MIW95_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY3153052.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            1867..3363
                     /locus_tag="MIW95_003761"
     CDS             1867..3363
                     /locus_tag="MIW95_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY3153053.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACISACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFEFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3497..3847
                     /locus_tag="MIW95_003762"
     CDS             3497..3847
                     /locus_tag="MIW95_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY3153054.1"
                     /translation="MKKIVLKDILFLVTTLSIIVILLFVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKNTAFKTTILSTGKSDCILIEIGDKFIMIDTGEDKNGEQIVDKL
                     KEKEINALDYLIKK"
BASE COUNT         1520 a          395 c          763 g         1184 t
ORIGIN      
        1 actatatata ttcgataaaa taaaatttaa acatataata ttttatgtat atattaataa
       61 aaggacatat tgtgttataa tggtatcgta taaaatcatt tacgggggtg gtattaaaat
      121 gtttcaattt gaaacacaaa tacacaaatt aagacatgat gttttaactg caatagctag
      181 acttggaaaa gaagataacc ttaccagaga agcaatggat aatattcagt atgaaataat
      241 aaaaggtgag aaacctacat acagatgttg tgtttataaa gaaagagcta taatatctga
      301 gagagtaaat gtaagtatgg ggttacaacc aggtcaagat aagatagatt tagatacaat
      361 ggatatagaa gaagatgaac aaattatata tattttggaa gctgcatgtg acagttgtcc
      421 aataaatgaa tttacagtaa cagatatttg tagaggatgt ctagcacata gatgtaaaga
      481 agcttgtaaa tttggagcta taacacatgt cggaggaatg gcctatataa atcatgaatt
      541 atgtaaggct tgtggaatgt gtaaaaaagc ttgtcaatat gatgccatct cagaggttgt
      601 tagaccatgt aagagtgttt gtccaactaa tgccttagga tttgatagag aaaatatgaa
      661 agctatgata catgaggata aatgtttaaa ctgtggagca tgtatgtcag catgtccatt
      721 tggagcaata tctgataaga gtttgatagc tcctgtagct agaaagttgg ttcaaaaaga
      781 gaaaatgtat gcggtagtag ctccagctat aacaggtcaa gttgaagcaa atgttacata
      841 tggtcaaata aaaaatgcta taaaatcact aggatttgtt gatgtattag aagcagcttg
      901 tggagcagat gcagttacag ttcatgaaag cttagaattt gctgaaagaa tggaaaaggg
      961 agaggctttt atgacaaatt cttgttgtcc aggatttgtt ggttatatag aaaaaacata
     1021 cccaaatcat gtaggaaaaa tatcttctac agtgtctcct atgatagcta gtggtagatt
     1081 tataaaaagt catgaaccag atgctaaagt tgtattcata ggaccatgta cagccaaaaa
     1141 gagtgaggca gctaagaaag aattaaaagg agctgttgat tatgtactta cttttgaaga
     1201 aataatggca ttatttgagg catttgatat agacttggct aagtgttcaa atgaagatat
     1261 agatgatgca tcaatttttg gaagaggttt tggagctgct ggtggtctta caaaagctat
     1321 agaaaattat cttgctgaaa aaggtattgg tgttaatttt gaacctgtaa agatttctgg
     1381 ttcaagagaa atcaagaaaa ctatgactat ggctaattta ggaaagctag aaggtaattt
     1441 tatagaagga atgatgtgtg aaggtggttg tataaatggt tctggtaaat tcttatcagg
     1501 tgcaaaggtt aaagccactt ttgatagaaa aaattctcaa tcaactaaga aaagtgtctt
     1561 agcaaataca aagataaaag attttagtaa tataaattta gaaaaataaa gataatacaa
     1621 agcaacaaaa atacatattg ttaatagagt tgttctaagt tgaggtttaa cttttggaca
     1681 actctatttt tatttatata tttaattaat gaacaactaa aataaaaatt acatatataa
     1741 tattttgtat ataacttaac aaagtatatg cctatgttat aataatttat agaaataaat
     1801 aaaaaataca atatacataa tagtagaaat atagtaaaaa ggtgtgattt atagggagga
     1861 ttacttatgt ttgaatttga aacgcaacta aaaaggttga aacatgaagt tttaacagca
     1921 gtagcaagac tatctattga agaaaattta acaaaagaaa atatagaaaa aattccatat
     1981 gaaataatta aaggagaaaa ggcgagatat agatgttgtg tttataaaga gagagaagta
     2041 gttcttgaaa gggcgcaact tgcattaagt ttaactccaa atagtaaata tggagatata
     2101 aatccagatt cagttagttt ggacacagat gagcaaatta tatatatagt tgaagcagca
     2161 tgtgatagat gtccaataaa tgaatttaca gttacagagc tttgtagagg gtgtttggct
     2221 catagatgta aggaagcatg taaatttggg gcaatttctt atataaatgg gagagcatat
     2281 ataaatcatg atttatgtaa ggcttgtgga atgtgcaaaa gttcatgtca atatgatgct
     2341 atatcagaag ttgtaagacc ttgtaaaagc gtttgtccaa caggagcttt ggattttaat
     2401 agaaacacta tgaaagcaat gatacatgaa gataattgta taaattgtgg agcatgtata
     2461 tcagcatgtc cttttggagc aatatctgat aagagtttga tagcacctgt tgcaaaaaaa
     2521 cttgcaaaga aagaaaatat gtatgcaata gtagctccag ctataacagg tcaaatagat
     2581 agtaatatta catatggtca gttaaaaaat gctataaaat cacttggatt tgtagatatg
     2641 atagaagctt cttgtggagc agatgcagtt acagttcatg aaagcaatga gcttattgaa
     2701 agaataaaat caggagataa ttacatgaca aattcatgtt gtccgggatt ttttaagtat
     2761 atagagaaga tgttcccaga tgaaaaagat agaatatcat ctacagtgtc tcctatggta
     2821 gcaacaggaa gattagtaaa aaaaatagat tctgatgcta aagttgtatt tatagggcca
     2881 tgtacagcta aaaagagtga ggcaacagag aaaagtttaa aagattcagt agattatgta
     2941 cttacttttg aagaactaat ggcattattt gaagcattta atatagattt atcaaaatgt
     3001 tcaaatgaag atataaatga tggttcgatt tttggaaggg gatttggagc gtctggtgga
     3061 cttgccaaat cagtacagaa ctatattgct gaaaagggac ttagttttga gtttgaacct
     3121 ataaaagtat ctggtccaag acaaattaaa aagacaatga caatggctaa attgggaaga
     3181 ttagaaggta attttataga aggaatgatg tgtgaagaag gttgtattaa tggagcaggt
     3241 aagtttttat caggtgtaaa agctaaaaat acttttgata gaaaaaatgg acaatctact
     3301 aaaaagagtg ttttatctaa tgataagatt aaagaatttg aagatgtaaa tttagaaaga
     3361 taattgtttt ggtacagtca tttttttatc atgccacaaa tatgcctaaa attttccctt
     3421 tttatatgtg tattttacag agttaaggtt gctatactat aaatatgtaa taaataaaat
     3481 ttataggagc ttttggatga agaaaatagt attaaaagat atcctgtttt tagttactac
     3541 attatctata atagtaatat tattgtttgt aacaaatata actactaata gtaagataaa
     3601 atcaacaagt gaatcttatt taaaaagtga caaaaatagt gtgaggcaaa atggtatcca
     3661 gaggagcaca aaaaatactg catttaagac tactatatta agtactggaa agtctgactg
     3721 tatattaatc gagataggtg ataaattcat aatgattgat acaggagaag ataaaaatgg
     3781 agaacaaata gttgataaat taaaagaaaa agaaataaat gcacttgact atttgattaa
     3841 aaaatagcta cgcacactcg tg
//
LOCUS       DAGQWR010000235         3833 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.239, whole genome shotgun sequence.
ACCESSION   DAGQWR010000235 DAGQWR010000000
VERSION     DAGQWR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.239"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            208..1839
                     /locus_tag="MIW95_003763"
     CDS             208..1839
                     /locus_tag="MIW95_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3153055.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(1974..2900)
                     /gene="fba"
                     /locus_tag="MIW95_003764"
     CDS             complement(1974..2900)
                     /gene="fba"
                     /locus_tag="MIW95_003764"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBY3153056.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            3230..3559
                     /locus_tag="MIW95_003765"
     CDS             3230..3559
                     /locus_tag="MIW95_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBY3153057.1"
                     /translation="MPQIKIRGINENDICKISKKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            3559..3762
                     /locus_tag="MIW95_003766"
                     /pseudo
     CDS             3559..3762
                     /locus_tag="MIW95_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
BASE COUNT         1531 a          467 c          648 g         1187 t
ORIGIN      
        1 tgataaaata caattaaaag caaaatgaaa tttttaacaa actataatca ttaaaattat
       61 atcttagtta cgttttaatg aaataaacta cttgaaaaga cgaatattat aaggagttga
      121 ttatatgtgc tgatggtatt tattgtaagc aaacaataaa ttttgtatgt agttcaataa
      181 attacttaat gaaaggtgcg aatattaatg gataataatt taatagggct tatagaacat
      241 gtagaaaatc cagccatttt atgtaaagaa tcaggcgaaa taatatactg taatcatcta
      301 atagatagta tttttagttt tctagatata aaaaagccta gaaatataaa tgaattagat
      361 tcaaattttg acaaaacaga aatactgaca gatagtaaaa agaaaatagc tttcagagag
      421 ttaaggatga ctgcacatat atacaatatg aaagataata ataatgaaaa taatatagtt
      481 tatttatttg agaagtctct aatatctgat aaagtaattg aagatataat agagcatata
      541 gatgaagttg ttgttgtgtt taacaaagat ggggtaattg aaaagatgaa cactgtcagt
      601 gatgagatat taccttttaa aagaactgag gtacttggta gaaatataac agaccttgtg
      661 agacaaggat tagttgaaga accaataata ttaaacatgc ttaaagtgaa gaaaaagatt
      721 tatagaaata tcgtttaccc agatgggaag cttattgcat atacggcagt tccaagatgg
      781 gattctaagg gaaagcttac aggaggagta ctaacaggga gagatatttc aagggttatt
      841 aagcttgaat ctcagataaa atatagtgat atatcagaag acacagagta tataagtcaa
      901 agtaagatta tggacaatat aaaaaaagtt gttaagagag ctgcagcctc agattcttct
      961 atatttataa atggagaatc aggtgtagga aaagaaataa tagcaagaac aatatataag
     1021 tatagttcaa ggagagacaa accttttata gctataaact gtggagctat accaaatgaa
     1081 ttattagagt cagagttttt tggatatgaa gaaggttctt ttactggagc taagaaaaaa
     1141 ggtaaaaagg gactttttga agaggcgaat ggtggaacta ttttcttgga tgagataggt
     1201 gagttaccaa tgcagatgca aaaaaaatta cttagggtta tacaagaaaa tactattact
     1261 agaataggtg gtagtaagcc tataaaaata gatgttagat atataagtgc tactaatatt
     1321 tctcatgaag accttagaaa taaccttaaa tttagacaag atttgtacta tagattaagt
     1381 gttattccag taaaaatacc tcctcttaga gaaagaaaag aggatatagt accattagta
     1441 aattatttct taaaacttta caatgaaaag tacaatagag aagtagaagt ttcacctaaa
     1501 gttattgagt tattagaaga atattcttgg ccaggaaaca taagagaatt aaaaaacatc
     1561 attgaaagat ttgtagtatt atcagctaaa aatgtaattg gagaagatga atttaacatg
     1621 ctgataaatt tagatatgat tgataatgaa acagatgatt tatcaccaat agtggtaaat
     1681 ggaattatga atcttaatga tgcatataaa atagtggacc aaataatgat aagtaaggct
     1741 ataaataagt atggttcaat aacaaaggct gctgaagtta taggtatata tccttctacc
     1801 atccatagaa agataaagag tggacatatt cacgtttagc tgtcagttta aaaagacaat
     1861 ataattaaaa tattataaga tacataattg atacaactta aaagagatgg cttaaaaata
     1921 aatttaatta ctttaagtca tctcttttct tttataaata tttaaaagtt atattagttt
     1981 atagagttag cagaacctaa aacatcatct atcttgcttt caactacagc ttgtatagca
     2041 tctctaccag caccaatgta ttttcttggg tcaaattctt ttggattttc agctaagaat
     2101 tttctgatag cagcagtcat agctagtctt aagtcagtat ccatgtttat tttacaaaca
     2161 gccatagaag atgcttttct taacatatct acaggtacac ctttagcacc agctatattt
     2221 ccaccaaatt cattacatgt agcaacagcg ttttggtcaa cagcagatgc accatgaaga
     2281 actattggga aacctggtaa tttgctttgg atttcttcta gtatatcaaa tcttaattta
     2341 gcttctcctt tgaatttgaa agcaccatga gaagttccaa tagctattgc taatgaatca
     2401 acacctgttc tttcaacgaa ctcaactgcc tcagctggtt gagtatactt gtgaacatca
     2461 gaagtaacat catcttctgt tccagctaga acaccaagtt ctgcttcaac aacaacacct
     2521 ttagagtgtg catattcaac agcttcttta gttattctaa cattttcttc aaagtcaaaa
     2581 tgagaaccat ctatcataac tgaagagaaa ccagcatcaa tacaagtttt aatagcatcc
     2641 atatttggac catggtctaa gtgaagagct acatctacac ctatttcatc agaagcagcc
     2701 ttaaccattt caactaatgt atgaggtcca gcatatttaa cagctgacat agaagcttgt
     2761 atcataacat aagaattttt agctttagca gcttttaaaa caccttgtaa ttgttctaag
     2821 tcacttatgt taaatgcacc tatagcaaat ccaccttcgt aagctttttt aaacatttct
     2881 tttgtagtaa ttaatgccat tttaaactcc tcctattaac ttttaaaatt tacccatcac
     2941 ttatattata acgttaaatc aaaaaaagta tatagtaaat tgtaataact ataaattaca
     3001 agtataatgt taaatagaag acaataggat taaataaaat aataaagaat ataaataatt
     3061 taggaaaatt agctatataa tgctaactat aagggtagga actaccttat tagtttggag
     3121 atactgataa caatagttgt cttgaccaag aagttaccac ttcaaacgtt atcgaaggga
     3181 attaagtgga tgataattca ctaaaaattt aaaataaagg agataaacta tgccacagat
     3241 aaaaataaga ggaattaatg aaaatgatat atgtaaaata agtaaaaaaa tgataaatga
     3301 tttagttgaa gctgtaaaat gtccaagaga ctactttgaa atagaatgta taaagtcagt
     3361 tgcaataaga gatggtaaaa tagcagatgt atatcctttt gttgaagtag cttggtttga
     3421 tagaggacaa gaagtacaag atatagtagc aagaataata actgatagta taagaaataa
     3481 tttagatgta gaaagtatgg atttagcatt tacagtattt gaaaaagaaa aatattatga
     3541 gaatggagag catttttaat ggctaaaaag caacgtatag ataaaattct ttcaaattta
     3601 ggttatggaa gtagaagtga aataaagaag tattgtaaac aagggtctgt agtagtaaat
     3661 ggaagtgaag tttcaaatcc aggaactcaa gtagacactg aaaatgatga gatattattt
     3721 aatggagaag aagttatata tagagaatat atatatttga ttaaaaaata gctacgtaca
     3781 ctcgtgactt tagtcatgag ttagtagcta aaatagtcag catataggga aac
//
LOCUS       DAGQWR010000236         3800 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.240, whole genome shotgun sequence.
ACCESSION   DAGQWR010000236 DAGQWR010000000
VERSION     DAGQWR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.240"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            78..1130
                     /gene="ytvI"
                     /locus_tag="MIW95_003767"
     CDS             78..1130
                     /gene="ytvI"
                     /locus_tag="MIW95_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBY3153058.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            1221..2447
                     /gene="pepT"
                     /locus_tag="MIW95_003768"
     CDS             1221..2447
                     /gene="pepT"
                     /locus_tag="MIW95_003768"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBY3153059.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            2654..3706
                     /locus_tag="MIW95_003769"
     CDS             2654..3706
                     /locus_tag="MIW95_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBY3153060.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT         1472 a          395 c          701 g         1232 t
ORIGIN      
        1 tttattttgt attcaaataa aactagtatg aaatggacaa aataaaaata taaattagaa
       61 gaaaggagat ttttaagatg agcatactag aaaataattt tttatacaaa ttaaaaaaca
      121 acactatctt tttttgtata tatacattgt tatttatttt tatttacaaa gcgtttccat
      181 acatagctcc attttttcta ggtgctataa tagcacttat gattaatcct atatcacaaa
      241 agttagaaaa taagtttcgt ataaataaag gtatctctac tcttgttcta agttttcttg
      301 cagtggctat agtcagtaca gttactacaa taatagtaat aaattcaatg aaagaattaa
      361 tgggattttt aaataatata tctgctaatc ctgaggatat aagtaatact attatgtatt
      421 ttttaaataa aataaatgac tttatgaaat catttcaaga aatagccaat tttgatgtag
      481 aacaattagt aaacaaattc agtggtgaag taatgcaaat tacaaaaaat cttcttacta
      541 gtatacttgg attggctact tctattccat atattataat atttataatt accttgttta
      601 ttgctaccta ctttatagca aaagaccttg ataagattga aaatagtttt tataatatgt
      661 ttactgttga tgttagaaag aaggtaaaaa acgtaaagaa agaggcaggg ctttcacttg
      721 ttggatatat aagggcctat acaatactta tggctataac attttttgca atctggggaa
      781 gttttgcttt atttggattg aaatacggtc taatagtagg gtttgtagga gctttaatgg
      841 atttaattcc attcctaggt ataataacaa tatatcttcc agtaattgtt tattattttc
      901 ttataaaaaa ttattttata gctataagta tgactgtgat attttttgtt ttgtctttga
      961 ttagagaaat acttgaacct aaactagtat cagtaaatgt tggactaaat ccattagcta
     1021 ccctagcagc gatttttatt ggaatccaag taaaaggaat aattggagta attttttgtt
     1081 tgggtttggt atgtatgcat gatatactta agaaggtgga tattttttaa ttataataat
     1141 tgatagattt ttttaaaaat aaaattttat tcacaggtaa ataaaaaaat attaatctta
     1201 ttaagaaaga gggtatatac gtgaaacaaa aagtagtgga aagattttta aaatatgtat
     1261 catttgatac aacatctaat tctcagtgtg agaactgtcc atcttcagaa ggtcaaagag
     1321 ttttagctaa gtatatagtt gaagagctaa aaacaatggg tgttgatgat gtgagtttag
     1381 atgaaaatag ttatataatg gctactttaa aaggaaatac tgatggtgtg gatactatag
     1441 gattcatatc acatttagat acaattgaag atgttagtgg aaaagatatt aaaccaagaa
     1501 taatagaaaa ttatgacgga aaagacatag ttttaaatga agcgttaaat gttattacgt
     1561 atgtaaagga ttctccagag ttggaagaat ttaaaggtga cgatttaata gtcactgatg
     1621 gaacaactct acttggctct gatgataaag ctggtatagc tgagatagtg actgcaatag
     1681 agtatctaat aaatcatcca gaaattaagc atggagatat aaaaatagga tttacaccag
     1741 atgaagaaat tggtagaggt gctgatttat ttgatgttga aaaatttgga gcaaaatatg
     1801 catatacttt agatggagga atagttggag aacttgaatg tgaaaatttt aatgctgcaa
     1861 atgcaactat tactatacat ggtagaaatg ttcatccagg ttcagctaaa aataaaatgg
     1921 taaatgctat acatatagct gctgaaatat cagaaatgtt cccagcagat gaaagaccag
     1981 aaacaacaga aggatatgaa ggattttggc atttaaattc tataggtgga aatgtcgaga
     2041 atgtaagtat ggcttatatt attagagacc attgtagaga aaaatttgaa aatagaaaat
     2101 ctattatgat agagaatata gaaaaaataa ataagaaata cgataataga gtggaattgg
     2161 atttaaaaga ttcttattac aatatgaaag aaaagattga gccagttatg tttatagttg
     2221 atatagctaa agaagctatg gaggaattag gtataaaacc acgtttagtt cctgtaagag
     2281 gtggtactga tggagcaaga ttatctttta atggattacc ttgtccaaat atatttactg
     2341 gtggattaaa cttccatggg aaaaatgagt gtataccagt tagttctatg gagaaagcta
     2401 ctaaactaat tgtaagaata gctgaaaaat atgctgaaag agtttagtta aaaagtattt
     2461 aaaaattaag catttaaagt tataatagct atatcattct ttataagtca agctgattat
     2521 aagcttgtgt tgtatttaaa atggtatagc tatttttgta attattttta taaaagataa
     2581 ttatttttag tttagggtat caataaaaag tatggatatc aataggtatt aacatttaaa
     2641 agggggaatt gtattgaaaa ttaaatctaa aaaaataata gcatttgcac tttcacttgg
     2701 gattatgttg ccaagttgtg tatatgcaga cactattagt aaaaatcaaa atgcacaggt
     2761 agatatgaac aattatcttc aaggtagaaa atctccaaag gaggaaaaac ttattggtga
     2821 agatagattt gatacagcga taaaaatatc tcaaagtggt tggaataatg gttcagaaag
     2881 ggtgtttcta gttaactcaa attcattgcc agatgcattg gcttcaactc ctttggcaag
     2941 taaattggat gcaccaatac tgcttacaaa taaaaatagt ataccatata atgtaacaga
     3001 ggaaataaaa aggcttaatc cagcagaagt tattttaatt ggaagtgaag gagctatttc
     3061 atcaagtgta aaagaatctc ttgaagatat ggaagtatta gtcagtagaa tcggtggagc
     3121 agatagagaa gaaacatctt tacttcttac tagacagcta gatgatactg gtgatttagg
     3181 tgtctcaaaa gtggcagtgg ttaatgggta taatggactg gcagatgcaa cttcaatatc
     3241 aagcccagct gcaagtgata atacagctat aatatataca ggtaaggata gcataagaag
     3301 tgaggcaaag aattttatta cacaaaactc aactaagaca tacatagtag gtggggagta
     3361 cagtataagt aaaaaacttg aaggacagtt agtaaactca gaaagactag caggaacaga
     3421 tagaaaagat actaatgcga aagttttaga aaagttttat ggaaaatcaa gtaaggttaa
     3481 taatatgtat tttgcaaaag atggaagtgg tagagaagct gatttagttg atgggcttgc
     3541 tgctggagta ttagcatcaa aaacaaagtc acctgtagta cttgcaagtg gaagtttaag
     3601 tagtgcacag aaaagtttta taaaaaaagt aaaggctgat aaatttgtac aagttggtgg
     3661 aggtaaaaat agtaaaccat atgcagaagc attagcctta cagtaatatt gtgtaaatca
     3721 aaattaatat ataataatag caagtaaacc aagttgaact tcaatatata taataaaata
     3781 agtaaactaa gttatatttc
//
LOCUS       DAGQWR010000237         3644 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.241, whole genome shotgun sequence.
ACCESSION   DAGQWR010000237 DAGQWR010000000
VERSION     DAGQWR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.241"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..1376
                     /locus_tag="MIW95_003770"
     CDS             <1..1376
                     /locus_tag="MIW95_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY3153061.1"
                     /translation="KLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIMILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIINLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            1379..1777
                     /locus_tag="MIW95_003771"
     CDS             1379..1777
                     /locus_tag="MIW95_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBY3153062.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            1817..3550
                     /locus_tag="MIW95_003772"
     CDS             1817..3550
                     /locus_tag="MIW95_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBY3153063.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGVGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKKILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
BASE COUNT         1548 a          300 c          606 g         1190 t
ORIGIN      
        1 ttaagttgta tgaggataaa attaatttag gattgataaa taattcagat aagtttataa
       61 atattactaa agatgagaac ttcctaaaac atgttcaaaa ttatattatc agtaatgaaa
      121 aagaaaactg tgtaatggct tgttttgatt tatgtagact caaacttata aatgatatat
      181 atggttatga gctaggggat gaagtgttaa ataatatttt aagtaactta aaaaattatt
      241 ttggaaaaga agctatttat ggaaagttaa aaagtgatgt ttttgtatta ataataaagc
      301 ttgaaaatag ggaacagaag attccttatt tagtaaatct tatcaagaat aaaattatga
      361 tattaagtca gattgacagt tttaaaatgg atataaatat tgaggcttct attggaattt
      421 ataaatttaa caataatgaa attgatataa aaaaagctat agataatgct gatatggcaa
      481 ggcttaaatc aaaaggacta aagcacatag agtatgtaga atttgataat gctatggaag
      541 aagaaataca atcaattatg aaaatagaga gagatttatt cttggctatt aaaaataaag
      601 agtttgtaat tcactatcaa cctaaagttg attcaagtac aggtaacatt ataggttcag
      661 aggcattgat aagatggttg cacccatcat taggcatggt tggaccaaat aagtttatac
      721 ctatagctga aaaaaatggt ctgataaaca atattggtag atggctaatt caagaagttt
      781 ttattaccat taacaagtgg atttcagaag gtataaatgt cttaccagtc tcaataaatc
      841 tttctagagt agaactatat aaaaatgatt tagttgattt ttttaaactg atgtttaata
      901 catataatat tccaaaagaa cttatagaat tagaaattac agaaacgaca gcgctaagag
      961 atgtaaaatt tataagtgaa aggctttatg agattaaatc tttaggtatg aatatatcat
     1021 tagatgattt tggagtaggt aattcaaatt ttatcaatct taaggggata ccactagata
     1081 ttataaaaat tgataggtca cttatattgg atatagttac aaatacaaaa acaaagttta
     1141 tggtgaaagc aattattaac ctttcacatg atttaaacgt aactgttata tgtgaagggg
     1201 tagaggatat gcatcaggtc aaagtcttaa gtgaactggg atgtaatttc atacaaggtt
     1261 atgtattttt taaacctttg gatgagatga attataaaaa acttcttact gacggaagtt
     1321 atataaatct gaaagatact cttttagata aatgcatgac agttgaggag gagtaaggat
     1381 gatttcagaa gatgtagatt attcaataag aatcataaga gctatattta ataatggaga
     1441 agataaacca atagatgcta aaatgatatt aaaaagtgaa gatataccaa tactaacact
     1501 atacagactg cttagaaagc tttgtgcaaa caatattctt acttttaaca aagaagatgg
     1561 caagacatac atgttaaatg tgtcattgga tacaataagt ctaagaacta ttgtagaagt
     1621 aattgatgga aaaatatata taaataaatg tacgagatat gtatctaatt gcagtaattg
     1681 cttaaaaggt tgtaaagtaa atcgtgaatt gctagaaata gaaaaagaaa tttataaact
     1741 tttatcgaga aaatctataa gacaaatact agaataaaaa attttcagct atgataataa
     1801 ttggaggttt tcggttatgg atgatatatc tcaagataat tttctactgt ctaaagagta
     1861 tgagaatagc ttagatgtag atactaaaaa agcaagtgga atatattata ctcctaaaat
     1921 aattgtagat tatattgtaa aaaaaacttt aaaaaatcac gatataatta aaaatccata
     1981 tccacgaata cttgatattt cctgtgggtg tggaaatttt cttttagaag tatatgatat
     2041 tttatatgac ttatttgagg aaaatatata tgaattgaaa aagaagtatg atgaaaatta
     2101 ttggactgtt gataacatac accgtcatat attaaactat tgtatatatg gtgctgatat
     2161 tgatgaaaaa gctataagta tattaaaaga tagcttaaca aataaaaagg tagtaaatga
     2221 tttagatgaa tctgatataa agattaatct attttgttgt gatagtctta aaaagaaatg
     2281 gcgatataag tttgattaca tagtgggtaa tccaccctat attggtcata aaaaacttga
     2341 aaagaaatat aaaaaatttc tcctagaaaa atatagtgaa gtttataagg ataaagctga
     2401 cttatatttt tgtttttata aaaaaattat agatatttta aagcaaggag gggttggcag
     2461 tgttataact ccaagatatt tcttagaaag tctatcaggt aaagatttaa gagaatatat
     2521 taaatcaaat gtcaatgttc aagaaatagt ggatttttta ggtgcaaata tatttaaaaa
     2581 cataggggta tctagttgta ttctaacttt tgataagaaa aaaacaaaag agacatatat
     2641 agatgtattt aaaatcaaaa atgaggatat atgtataaat aaatttgaaa cattggaaga
     2701 acttctaaag agtagcaaat ttgaacattt taatataaat cagagattgc tatctgacga
     2761 atggatactt gtaaataaag atgatgaaac tttttataat aagatacaag aaaaatgtaa
     2821 atatagcttg gaagatatag caataagttt tcaaggaata attacagggt gtgataaagc
     2881 gtttatttta agtaaagatg atgtaaagtt gaaccttgta gatgataaat ttttaaaatg
     2941 ttggataaaa agtaagaata taaataaata tatagtagat aaaagtgaat ataggctaat
     3001 atactcaaat gatatagata atgagaatac aaataaaaag atactagatg aaattatagg
     3061 gctatataaa acaaaattag aaaacagaag agaatgtaaa agtggaataa ggaaatggta
     3121 tgaacttcaa tggggtagag aaaagctttt ctttgaaaga aaaaagataa tgtatcctta
     3181 taaatcaaat gaaaatagat ttgctataga ttatgataac aatttttcaa gtgcagatgt
     3241 atattctttt tttataaaag aagagtattt agataaattt tcatatgaat atctagttgg
     3301 aattttaaat tccagtgttt atgataaata ctttaaaata actgctaaaa aaatgagtaa
     3361 aaatatatat gattactatc ctaataaggt tatgaagata aggattttta gggataataa
     3421 ttatgaagag atagagaatt tatcaaagca aataatatca attttattaa ataagtctat
     3481 agataaagga aaagtagaaa aattgcagat aaaaatggat aacttgataa tggattcttt
     3541 aggtatataa gcagttgtaa gtattaaaaa attataatcc tttaattgtt tatttaaatt
     3601 tagaaataaa aagttagcca tataaaaaat attaaaaata tata
//
LOCUS       DAGQWR010000238         3632 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.242, whole genome shotgun sequence.
ACCESSION   DAGQWR010000238 DAGQWR010000000
VERSION     DAGQWR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.242"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     repeat_region   658..1014
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=658..686
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            1253..1441
                     /locus_tag="MIW95_003773"
     CDS             1253..1441
                     /locus_tag="MIW95_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153064.1"
                     /translation="MKIITDSSRADYFKQRRQNKKTFSVLLDREKVEKIEEHLKKQNK
                     TKTIWLEEKINEELEKEE"
     gene            1560..2426
                     /locus_tag="MIW95_003774"
     CDS             1560..2426
                     /locus_tag="MIW95_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HBY3153065.1"
                     /translation="MKNLIVKEFNGSQIYTFMWKEKSCWIANQIVGLFDYADVSKTIQ
                     DCIKAEDFEIEQEYDVLKGNEFNDFVTTLNVVANNIISNKARSITIFYEDGLYGFLQY
                     TDKPIGVQFRKWLRREVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLK
                     ELLDGAGFDNKSKLLTAKTLYKKAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPA
                     QLAVCEIIKKIDLEENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPTKIAG
                     EKKNYHVVYKIE"
     gene            3291..>3632
                     /locus_tag="MIW95_003775"
     CDS             3291..>3632
                     /locus_tag="MIW95_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBY3153066.1"
                     /translation="MREGKRRRGCLFWFILIILFSGVVGAIAGNSTNNESTEKQKEDL
                     TKYIGEEGSIGDLKLTVNSISKASEISVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPD"
BASE COUNT         1466 a          350 c          618 g         1198 t
ORIGIN      
        1 aaaatattgt ttatataaaa taaaaaataa aaatttttat taaaaaaatg aaaacttgat
       61 tataaagcaa ttaatttata aaatatatat aaataagtag gtatttattt acttgaattt
      121 cattgtttat ataaaaaaaa tggaaaaata tgtaataatt ataggttata ataattgtag
      181 caaggataat aatcgaaagt gcgaagggtg attattttca tattaaacgc caaattccaa
      241 ataaggaagg aggtgaaatt atatgatagg ttttttatta agcatactag ctggtgttat
      301 atcagcttat atttatgaca aaataaaaaa tcacccagac gccaataagg gtgatttaaa
      361 aaaataatat tttcacttaa caactgaaaa taatcactct ttgtaggaat aaattatttc
      421 cttgctttta ttataccaca aattggtaca gatattcaaa aataatattt ttatgatata
      481 ataaaaatgt agagattttg cagtgagcga tatttgtgat aaattgaagt ttaacagttg
      541 caatataagg cgttgagggt gtgtgataaa tgttatcaat tgcactactc atggttcact
      601 gcaaatttaa gagagttgta tatgtgtagg tattgaaaat actcaactta ttttggggtt
      661 ttagattaac tatatggaat gtaaatgttg taagaagtat cattctattt tttaatcttt
      721 ctgttttaga ttaactatat ggaatgtaaa tttcagtgag aataagcttt attgtcgatg
      781 taacactcgt tttagattaa ctatatggaa tgtaaatagt acatataatg agtctttaac
      841 atcagttatg aaaggtttta gattaactat atggaatgta aatgattgta ctttagcgtc
      901 tgcactagct ttgtctatcg ttttagatta actatatgga atgtaaatta ttttacagat
      961 gaacaattac agttacttct tgaatgtttt atattaacta tgtggattca aaattaaata
     1021 gaaaaaagaa agcacttact taaatagtag gtgcttttgt tttgctcaaa ttacttaatt
     1081 ataaagttgc taagtacaac tttttagaat tatacaatat aaaaatgcac taaagtgatt
     1141 gattttgctc aagttggtcg gttgagtaaa ataattagaa aaaaatgtta aaaaactctt
     1201 gaaaagtgtc gcgatacaat gtataattat attatcgcga tacagaaaag aggtgaaaat
     1261 tattactgat agcagtagag cagattactt caagcagaga cgacagaata aaaaaacttt
     1321 tagtgttcta ctagatagag agaaagtaga gaaaattgaa gaacatttaa aaaagcagaa
     1381 caagactaaa actatttggc ttgaagaaaa gattaatgaa gagctagaaa aagaggaata
     1441 aaaaataaga gacgttctcc ccgaccaaag attgaacatc ccttattgac gtatattata
     1501 tacactaact atagtatacg tcattccttg aaaaaattca attaaggagt gtaatagtta
     1561 tgaaaaattt aatagtaaaa gagttcaatg gaagtcaaat ttatactttt atgtggaaag
     1621 aaaaatcttg ttggatagct aatcaaatag ttggattatt cgattatgct gatgtatcta
     1681 aaacaataca ggattgcata aaagcagaag actttgagat tgaacaagag tatgatgtat
     1741 tgaaaggaaa tgaatttaat gattttgtaa ctactttaaa tgtagtcgca aataatataa
     1801 ttagtaataa agctagaagt ataactattt tttatgaaga tggtttatat ggatttttac
     1861 aatacacaga caaacctatt ggtgtacagt ttaggaaatg gcttagacga gaagttttac
     1921 caagcataag acaaactggt gcatacataa ctaacaatgc taatcccgaa aaactaagag
     1981 aaaaagcaag tgagattgag aaattacagt tagcttacaa tagtacatct atgttaaaag
     2041 aactattaga tggtgcaggc tttgacaata aatccaaact attaacagct aaaacattat
     2101 ataagaaagc aggaattgat ttaccaatag agataaacga agaagaacat tattttgata
     2161 caaagcaaat agcatctaaa ctgaaaatat attctaagag taataaacca gcacagttgg
     2221 ctgtttgtga aattattaaa aagattgatt tagaagaaaa cgaagtcaaa ggcgtttggg
     2281 aaactaatgg ttcttggact ggtactgtaa ataaatatac aaagagtgta atagataagg
     2341 ttagaaattg gatagaggaa aataatagac ctactaagat tgcaggtgaa aagaagaatt
     2401 atcatgtggt ttataagatt gagtaaattt ataagattgt attaaataat ttagtttagt
     2461 tttgggggga ttaatacaat gcatgagaat ttacttgata gtatagatat tgaaaagaga
     2521 aaagaagaat ttagaattaa acttttgaaa ataagagaaa cagatataga tatatataat
     2581 aagatagaaa gtatagtata taaactttct tagaaaaaat tagagaaaaa taattaaata
     2641 aatagataaa gcacttggat atttcactgt ttcaagtgct ttatatggta aaaaatggta
     2701 taataaaata taggaatttt gcagtgttcg atttttctac tcaagtatag tttaacaatt
     2761 ggaatacaag gcattgagaa ggtttgataa gtgttatcaa ttgcactatt acccgctcac
     2821 tgcaaatttg agaaagttat atgtgtgtaa gtcttggaaa tacacaattt attttggggt
     2881 tttatattaa ctatatggaa tgtaaatctt tgtaatggta gtgtatttaa gattgaaaca
     2941 tcaagtttta tattaactat atggaatgta aatcaacatt agtagttgtc tttatacaca
     3001 tagcatcacg ttttatatta actatatgga atgtaaatac agctcccaag acatacaacg
     3061 aatctgtaac atcagtgttt tatattaact atgtggtatg taaaagactt atttacagct
     3121 ttatttgcta aatcagaagg ttttatatta actatgtgga cttaaaatta aaaataatta
     3181 aaaaacactt actaatgtag gtgttttttt attgaaagga agtgattata atgtaaaaat
     3241 tttgaagata tagtataata ttcttataaa atataattta gggggatatt atgagagaag
     3301 gaaagagaag aagaggatgt ctattttggt ttattcttat tattttattt tctggagttg
     3361 ttggagcaat agcagggaac agtactaata atgaaagcac tgaaaaacag aaagaggatt
     3421 taactaaata tattggtgaa gaaggtagta taggagattt aaaattaact gttaatagca
     3481 tttcaaaagc tagtgaaata tcagtagcat ctggttattt agcatacact ccagatagtg
     3541 gcaaatatgg tattataaat ttaacaataa aaaatcaaac taaggaaagc caatctttta
     3601 tgctaaattc attcacatta ataggaccag at
//
LOCUS       DAGQWR010000239         3389 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.243, whole genome shotgun sequence.
ACCESSION   DAGQWR010000239 DAGQWR010000000
VERSION     DAGQWR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.243"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(145..666)
                     /locus_tag="MIW95_003776"
     CDS             complement(145..666)
                     /locus_tag="MIW95_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBY3153067.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENIIIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
     gene            794..2158
                     /locus_tag="MIW95_003777"
     CDS             794..2158
                     /locus_tag="MIW95_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY3153068.1"
                     /translation="MKESNKKIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDKLHIAIKTSILWTTIFCVVLGL
                     ILVLFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            2347..3024
                     /locus_tag="MIW95_003778"
     CDS             2347..3024
                     /locus_tag="MIW95_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBY3153069.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSDYKKGNPVH
                     CGDSDCIGILIIKSGTIRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQINSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
BASE COUNT         1082 a          463 c          516 g         1328 t
ORIGIN      
        1 aaaagtttaa cattctccct caattaagtg agctttattt taaatatact tttcagttta
       61 tatcatttgt ttcatttttt ctaatatctc attattaaat atgctggttt ttgtttaatg
      121 attattaagt tagcttctct aattctaatt ccccatatta tcttttaaag aatttatcca
      181 ggtttccaat gcttgtgcta gcaccaactg ttgctccaac actgcttttg ctgtctctgg
      241 tacttctgtc tttataatta tattttcctc aatagttttc tttaactctt gaacattctc
      301 ttcaatatta ttgagacata atttctgttt ttctggtgta aggctattta aatttactat
      361 aacagcatta aaatctaaaa gaatatttat tggtttgcta gatgtatcaa acattaaatc
      421 taaaaaatgt gtctcaccta attcagttaa tgaataaact gctttttctg gcatttttcc
      481 attcttcaca gttttacttt caatatatcc cttttgttct aattgaatta cttttttata
      541 aattgatggt gtacttattt taacccaacg tgatatattc ctgtattcca ctgccttttg
      601 aatatcataa gcactcttgg gttgttgctt tactattccc aatacaatta aatctattgt
      661 agccataaaa atcatctcct ttagttttct tactattgac aaatactata aattatagta
      721 ctattttcca tacttactat aatttatagt attataattt agacttaatg tcaatagaag
      781 aggagtgtta agtatgaaag aaagtaacaa aaaaattgaa ttattagggt ctgccccaat
      841 ctcaaaagca ttgttatcta tgggattacc taccatgata ggtatgatga taactgcatt
      901 atacaatttg gttgatgcat actttgtggg tggacttgga acaagtcaaa tgggagctat
      961 atccgttgca tttccattag gtcagcttgt tgtgggatta ggattattgt ttggaaatgg
     1021 agctgcttca tatattgcca gacttttagg ccatggtgat aatgaaaaag caggaaaagt
     1081 ggtaagtaca tctatttaca gtagtattat agtgggtaca ttagttatta ttgtgattat
     1141 gatttttctc aatccaattt taaaattgtt aggagctaca aaaagtatta tgccttatgc
     1201 aatatcttat gctagcattt atgtcgtgtc atctattttt aatatattca atgtcactat
     1261 gactaatatt gtcacaagtg aaggagctgc taaaacaact atgtgtgcta tgttattagg
     1321 tgctttgctt aatatgattt tagacccgat attcatttat acttttggtc tgggggtaag
     1381 gggtgcttct attgcaacag ctatctcaca gattgcttca acacttgttt atgtaggata
     1441 tattttaagc aagaaaagta tatttagttt tagaattaac gaatgctgtt tttcaaaaga
     1501 aattttttca gaaatcctaa aaattggcat acctacatta atatttcaat tattgactag
     1561 tcttgccatt acattaacta atatggaggc tacaaagtat ggtgattctg tgatagctgg
     1621 aatgggtgct gtaacacgaa taacatcttt aggtagtctt gttgtatttg gttttataaa
     1681 aggtttccaa ccaattgctg gttataattt tggttcgaag aactacgata aattacatat
     1741 agcaataaaa acttccatat tatggactac aatattttgt gtagtgcttg gtcttatatt
     1801 ggtgcttttc ccaaaatcta taatttcaca atttacaagt ggtgacattc tattaataga
     1861 aaatggtagt aaagctctta gggcaaatgg gttttcattt atgctatttg gattctatac
     1921 agtgtattct acattattcc tagcactagg aaaagcaaag gaaggtggat ttttaggtat
     1981 gtgtagacaa ggcgtctgtt ttatccctat aattttgatt attcctgtat tctatgggtt
     2041 aaatggtatc ttatatgcac aaccaatagc tgatttgatg gctgctataa ttgctggttt
     2101 tatgtctata aggttacata aggaacttaa tcttttaaaa ataaacggta tgcattagtt
     2161 atacaaacct actatcatca aaacatatta ctttctcatt aatagcaaga atatatatta
     2221 tcatattttt tctacataaa tcatactatt actatctgac taaataatcc tcttaacaaa
     2281 ctacttgtca atttatctat tcacttgtat catgggatta taacaattta agaaaggaga
     2341 tattgtatga tttcacaaaa tgaaattgat atacttacac atagcttgcc tttttgggat
     2401 aaacttactg acttacaaaa agaattatta atttcttcag caaatacctc ggattacaaa
     2461 aaagggaatc cagtacattg tggtgatagt gactgtattg gtattttgat tataaagtct
     2521 ggaacaatac gtacatacat actttcagat gaaggtcgag aggttacact gttccgactt
     2581 gatgatggag atgtatgtat tttatcagct tcctgtattc taaaaacaat aacttttgat
     2641 gtctatgtag atgctgaaac ggactgcgat attatacaga ttaattcctc tgtttttgca
     2701 aaattatcta cagaaaatat tcatgcagag ttattttcat ataaattagc taccgaacgt
     2761 ttttctgatg ttatgtgggc tatgcaacaa attcttttta tgagttttga taagcgtttg
     2821 gcatcttttt taattgatga gatagctaaa aatggcagta gcactataaa tatgactcat
     2881 gagcaaattg ctaagtatat gggtagtgcc agagaagttg tatcgagaat gttgaaatat
     2941 tttgctagag aaggtattgt gtctttatct cgtggtggta taaaagtatt ggataaggat
     3001 aaacttcgtt ctttgacact gtaaaagtgt gtctcaaaat gaacttttta gttcatgagg
     3061 cactcttttt tgtgtgaaat caaatatatt ttcataattt caataataaa aaagatactt
     3121 atatctattt ttatatagta tcttatttga tatttacatt tattttttat gtatattttt
     3181 atattttaat aatttagttt aactattttt ctatgctatt tatatagaat atttctaata
     3241 actcataaat tctaatcttt taatatctat ccctcaatta atggatatga atttatttaa
     3301 tttaaatact tgatattttt catatttata taaaaatatt acattttaaa tacaagttaa
     3361 taaaaatgga agaatatatg ttaatgtaa
//
LOCUS       DAGQWR010000240         3353 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.244, whole genome shotgun sequence.
ACCESSION   DAGQWR010000240 DAGQWR010000000
VERSION     DAGQWR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.244"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(458..679)
                     /locus_tag="MIW95_003779"
     CDS             complement(458..679)
                     /locus_tag="MIW95_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBY3153070.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            1259..1516
                     /locus_tag="MIW95_003780"
     CDS             1259..1516
                     /locus_tag="MIW95_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBY3153071.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            1965..3203
                     /locus_tag="MIW95_003781"
     CDS             1965..3203
                     /locus_tag="MIW95_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBY3153072.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKRNKSATKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1354 a          301 c          509 g         1189 t
ORIGIN      
        1 tcaatattag tgttttgctt gttagtatta gtatttcaat tgtcaatatt agtgttttgc
       61 ttgttagtat tagtatttca attgtcaata ttagtgtttt gtttgttagt atcagtgttt
      121 caattgtcaa tattagtgtt ttgtttgtta gtatcagtgt ttcaattgtc aatattagta
      181 ttttaattgt tgactctagt tattgctgat atagaaaata tttaatttat ttcaattaat
      241 tagattaaag tagggtagca tttaaaatag ctaacatgtt taagttgaaa tacgtttaat
      301 atattaattt ttcaaagcat taatatatta aagttataac caaaattgaa gttataatct
      361 gccaaatcta tagcgtatta agtgcgttat ataacttaat cttttatata aagagttaat
      421 ttttttaatt tgtaaagcag agtacgttta aaagatacta tttagataat gatgtactat
      481 ctaattttaa aatatctaca atagggttct cttgaagact tgctattgct tctgcaacag
      541 cataaacatc ttcatttaat gcctctgctt ttacatttgc aaatgattta cttttaacaa
      601 ctgccttacc cttatcatca aaaccagcat ccaattttag ttttatacta gataaatttt
      661 ttgttgaaac aacttccata aaattacctc ctaaaaattt aatttcaaag atttgtcgac
      721 tttctttgtt agctataata ctatcatcta agataatatc ggagttaaac agtaatttct
      781 aaagattttt tgtgtaaatt tttataggaa aaattttact tttctgatga tgaaaaagtt
      841 tacaagttat ttttgaagat atgttcttag tgttttgaaa tagtatttgt atatattcat
      901 gcttagttga gatagttttg tatggatttt catatgtgta gagttaaatg ttaggtacca
      961 aagtttatgt taaattaaat gttaaagctt atgttaaaaa ttttattaaa tttaatctgt
     1021 aaataaaaaa gttaaaagtc caatattaac attttacttt taaaccatta aaatctttat
     1081 taaagctaac aaagttataa gttatgagaa attttattaa agttaataca aaaattaatt
     1141 ttaaagctaa gaaatttttt attagattca ataaaactaa taaatatagt ttagtcaata
     1201 atgtattaaa taaaaacttt gttaagtcta taaaaatatc ttaaaggagg tgtaacttat
     1261 gaagataaaa gtggaggata taccagttca atttcatgca atggtagaaa taatcggaat
     1321 tgataagttc atagaggttg ccaagttgta tggaggaaca aatacttata tacctacgta
     1381 taaggggctt tttagatatg ctagaaatag ggatatagta aaacaattta atggagtcaa
     1441 ccataatgaa ttggctatta gatataatat gtgtgtaagc aatattaagc gaatactcaa
     1501 tgaaaataat gtttaaacat aatgaaaata gccttgttaa atgtaagagt aaagacaaag
     1561 aagatgtttt ataaagtttt tttagacaaa ttaactaaat ttactactta ttaatttaaa
     1621 tacattgtat attctataaa aaaatttgtt aaatactgga gatatattga gaatacaaag
     1681 aaaatggtcg aaaatatttg ttggctaaaa aatagataaa atgatacaat tgcttgtata
     1741 aaggggaaaa ataaataaat aaactatata aataaagaaa tagcaattaa tatgacataa
     1801 tatatttata aaattaagaa tataaataga tattttaagg gattagttgt taaattagga
     1861 aatattaaaa aaatatatat atttatattt aaactatttt gttatttata taaaaatact
     1921 tatctagtta ctagcaatta ttagtagaaa tgggggaaat agagatgaat aagggatttt
     1981 acaaattaaa ttcagtggta ttgaactttg atattaagaa tgatgttaac aagataagat
     2041 ttaatcatta tataatgaga aaagaaaaca tacaaagact tcaaggaact ctgccaaacg
     2101 gacaatttta tatgacagtg agaagagctg ctacagattt acaactttct atttctacta
     2161 taagtaggct tgtaaaagag tttttggatt tagggataat aagattagtg tctaagggtg
     2221 taaaaggtaa tcgttctgta tatagttacg taagtataga gtttaatgga agtttatttg
     2281 atgatagcaa atatatggaa cttgactgtg tatgtaatga tgaatttatt gataatagtg
     2341 gcatagagtt tgattgtgat agcaaagata attcttttaa tggagatata aatgtagaaa
     2401 ctgtgcattg cattaatgag tatttctctg atgatgatgt agagcataat tataacaatt
     2461 tccataataa agatactata aaggataaag ccatttctaa tattagcact atcaaaaaat
     2521 atattgatga atcttttgat acaaataaat cagaaaattc taacttacaa caggagtcaa
     2581 taaatttcaa ctttaatgat acatgcaatg aaatagataa aagaacgaat aatagtatag
     2641 aaaataatca aaataaaagg cctatgagat tgagtgacat cagtgtttta agtgataaag
     2701 gtatgacaaa taaaaagaat tactttaatc ataatcatgg tatacaaaaa aggaataaat
     2761 ctgcaacaaa gaaaaaagaa tccttaaata aatttaataa aaaaaatata cagcaaagca
     2821 ttataaatat gctaaataaa aaatcaggta agaattttaa aatcgattct ccaataagca
     2881 taaaacttat caatgaaagg ctaaaggagg gctatgtctt agaagatttc tataaagtta
     2941 ttgaggtaaa agtggctaag tggaaaaata ccataatgga aatgtatata agaccagaaa
     3001 ccttgtttag ctataagttt gaatcctatg taaatgaaaa catatctatg aaaagtcaaa
     3061 gtaatgttca tccatgtaaa gaaggcaata gtaatgatag cagtaattat atacattctg
     3121 attatacaac atatgatggc agtgtgcgtg agagaaaaaa atatgagaat gtaagatact
     3181 ggaatcctat atgtgaaatt taattagttt aagaatgtat ttatgataaa taagatatta
     3241 atagagaata gtgagctata gcaaatgata tatcaatagt tggtagtaag ttatattaag
     3301 caagatatta ctatcaggta gtgaattata gtgaacgata tatcaataat tgg
//
LOCUS       DAGQWR010000241         3336 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.245, whole genome shotgun sequence.
ACCESSION   DAGQWR010000241 DAGQWR010000000
VERSION     DAGQWR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.245"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..1076
                     /locus_tag="MIW95_003782"
     CDS             <1..1076
                     /locus_tag="MIW95_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBY3153073.1"
                     /translation="VIDLYDDNNNEVAKKVNFSIFNPKFNLYMYQYKNLINYLDKSIC
                     MGALSSGSTSVPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMS
                     VLYEGGSIVISSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTG
                     SQLLFEYTARNLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIY
                     IRGGKIFVNTEYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIVNIGGFKVSSAKIE
                     NEVKKIPQIEDAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKI
                     IFLNSFPYTSSEKIDRLALLKML"
     gene            1152..2027
                     /gene="dpsA"
                     /locus_tag="MIW95_003783"
     CDS             1152..2027
                     /gene="dpsA"
                     /locus_tag="MIW95_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBY3153074.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEVGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            2030..2620
                     /locus_tag="MIW95_003784"
     CDS             2030..2620
                     /locus_tag="MIW95_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBY3153075.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(2828..3028)
                     /locus_tag="MIW95_003785"
     CDS             complement(2828..3028)
                     /locus_tag="MIW95_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153076.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
BASE COUNT         1277 a          403 c          496 g         1160 t
ORIGIN      
        1 acgttattga tttatatgat gataacaata atgaagtagc aaaaaaagtt aatttttcta
       61 tttttaatcc taaatttaat ctatatatgt atcaatataa gaaccttata aattatttag
      121 ataaaagtat ttgtatggga gctttatctt caggttctac aagtgttcca aaggtattat
      181 atagaacata tgagagttgg gctggatttt ttcctataca aaatggtatt tttaatatct
      241 ctggggaatc aatcttgttt gttaatggaa cacttagttt tacagggaat ctaaattcta
      301 taatgtcagt attatatgaa ggaggtagta ttgtaataag ttcaggtctt aattgtaaat
      361 catggataag gacaataaaa caatataatg taactaacat ataccttata cctacaaaac
      421 ttcaactttt ggttaaacac ttaaaagaac cagtactcaa agtagtaagt atttttacag
      481 ggtctcaatt attatttgaa tatactgcaa gaaatctaaa aaaatatatg ccaaagtctg
      541 aaattatttt atactatggg gcaagtgaat taaattatat tacatattta tgctatgatg
      601 aactaattga aaaacctcta agtgtaggca gaccatttcc aggaatagat atatatatta
      661 gaggaggaaa aatttttgtg aatacagaat atgcagtata taatgcaacc aaaccttatt
      721 ctgtcaatga tattggatat tatgataatg atggatactt aatatttgaa ggaagaagtg
      781 atgacattgt aaatattgga ggatttaaag ttagttctgc taagattgaa aatgaagtta
      841 aaaaaattcc tcaaattgaa gatgcaattg tattgccgta ctcagacact ataagaggaa
      901 atcaaattgt attatttgta ataactatag acaaaattac gaagaaagat ttgttgatta
      961 aaatgaaaga taatctaatg aaaaatgaaa tcccaaagaa aattatcttt ttaaattcat
     1021 ttccatatac ctcttctgaa aagatagata gattagcatt attaaaaatg ttataagtat
     1081 aatatttagt aattttagtg aaccaatgta ttgataaaaa catattgtat atagtaaagg
     1141 aggcttacaa tatgtcaaat caatacgata taactgttat tgggggagac ctgcgacaag
     1201 tgtacatggt aaaaaaacta attaataaag ggtattctgt agtcacttat gggatatcaa
     1261 atgaaattat aaatggaatc gttggaaaat caacttcact agctgaagca ctatccaaaa
     1321 gttccattat tttatgtcca attccattta caaagaatca agttaatatt gaaaatacgg
     1381 atgattgtct tgatgctact attgataact tgctaattaa tttatctcct aatcaaatat
     1441 tatttggagg taacatacca actaaagttt gtaaattttg cgagcaacac tctattcaga
     1501 tttatgattt tatgaaaatg aatgatgttg ctattttaaa tgcagttgct acagctgaag
     1561 gggctatagc agaagctatt aaaagaagta taatcaatat acatcaaagt aactgtttag
     1621 tattaggtta tggaagatgt gcacaaatac ttgcaaaaaa attatacgct ttggatgcaa
     1681 atgtatgcgt tggtgcaaga aataatgatg caagaagtac ggctaatgca tttggatatt
     1741 ccagtatttc tttaagtcaa cttgatgaaa aacttttaca atttgattat atttttaaca
     1801 ccataccaga gtgtatttta acgaaagaaa gattagaaaa agtatcacaa gaagtaacta
     1861 ttatagatat agcttcttct cctggaggag tagattattc tgttgttaaa gaagtaggga
     1921 ttaatgcgac tctttgcctt gggcttcctg gtaaatattc accaaagaca tctggtgaga
     1981 ttcttgttaa cgctatagaa aatattataa atgaaagaag ggattagaaa tgagacttga
     2041 aggacttaca attggagtag gatttactgg ttcgttttgt acttatgata aaatatttat
     2101 agagttagag aatttggtta aagaaggagc taatgtacac actatatttt cagatgtatc
     2161 acaaaatatc gattgtagat ttggaaattc tgaagagttt atgaaaaaag catatgaact
     2221 tacaggaaat aaacctatag ttacgattga agaggcagaa ccatttggac ctaaagggat
     2281 tgctgatatt ataataatag ctccttgtac tggaaatact gctgcaaaat tagcaaatgg
     2341 tattacagat tcaccagtat taatggcagc aaaaggtcat cttcgtaatg ataaaccact
     2401 agtaatatca atatcaacga atgatgctct aagctttaat ttcaaaaata ttggtattct
     2461 ccttaattct aaaaatatat attttgtacc ttttggtcaa gataattgca aagcaaagcc
     2521 taattctatg attgcacata ctgagctaat aattccaact atagaacttg ctctagagaa
     2581 caaacagcta cagcctgtta taaaaagtcc acatcaatag aattggttct tataatatta
     2641 tgtattagat acaatactag caaagaggct gtctcaaaat agagagaagc ctctttttca
     2701 ttgttgaaat gatgaaaata tatttaattt catacaaaaa aatgcatcat gaactaaaaa
     2761 gtctattttg agctaatctc cgtaattttt ggtatattaa aattatttac ttaatccaat
     2821 ttttaaatta gaatttatat cgttttgact cttcaaatat caatctaact gttgttgata
     2881 caaaagaaga ggacataatt aaaacaaata ctgatagtcc tctaataaac aaattatcta
     2941 cataataagt tactattcca atacctccaa tcgttaaaat tacacttata atcaaagaaa
     3001 aaatgctaat tgtttttaat tttttcatgc ttatcctcct tcttataaac ttcaaattta
     3061 tctatataat attaaatata ttttatttca tagtatataa aaatcatgaa tatatatttt
     3121 tttaattata ctatatgttg taaatgtttg tacatttaac tatatttact ttaacttgtt
     3181 aatcaagtta ttattttcat tttcaagtat ttttattttt aaacttgata gaatatcata
     3241 ttaaaaattc tttttataaa gttttatttg caaaaaatac aaaaatataa acgttaaaaa
     3301 aataacgtta tccttaagtt tttagatgat tttatt
//
LOCUS       DAGQWR010000242         3331 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.246, whole genome shotgun sequence.
ACCESSION   DAGQWR010000242 DAGQWR010000000
VERSION     DAGQWR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.246"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            94..1692
                     /locus_tag="MIW95_003786"
     CDS             94..1692
                     /locus_tag="MIW95_003786"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBY3153077.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFDVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1927..2118)
                     /locus_tag="MIW95_003787"
     CDS             complement(1927..2118)
                     /locus_tag="MIW95_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HBY3153078.1"
                     /translation="MAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQ
                     SAGTAATLTIFRIADTVMT"
     gene            complement(2287..2880)
                     /locus_tag="MIW95_003788"
     CDS             complement(2287..2880)
                     /locus_tag="MIW95_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HBY3153079.1"
                     /translation="MRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYY
                     NLRTITWNDRVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNY
                     PYQIFEFTSSRDCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSIGPMG
                     ATGPTGATGNSSQPIANFLVNAPSPQTLNNGNAITGW"
     gene            complement(2881..3027)
                     /locus_tag="MIW95_003789"
     CDS             complement(2881..3027)
                     /locus_tag="MIW95_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HBY3153080.1"
                     /translation="MRKIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFF"
BASE COUNT         1197 a          469 c          456 g         1209 t
ORIGIN      
        1 aatttttttc tttttacaac aaatactcac taccttttga tataatattt ttatcaatta
       61 ttattatatt caagttttga aaggagtatt aaaatgagtt tattagtttt cggacataaa
      121 aatccagata cagattcaat atgttctgca atttcattag cacatttaaa gaacaaacta
      181 ggaataaaag caacagctta tgctttagga gatatcagaa aagaagctaa atatgccttg
      241 gattacttta aggtagatgc acctgaaatt ttagataatg ttagaataca agttagagat
      301 ttaaattatg ataaagtagt acctttaaca ccaacttcat ctatattaga ggcttataac
      361 cttatggatg aaaaaaatgt taaaacacta cctgtaactt atgatgatgg aacttttgct
      421 ggtatcatta ctatgaaaga aatagctaaa aatctacttc atcaacactt tacaactata
      481 cacacatcac tatcaaacat ttgtaaaaac cttaatggta ctattcttgt ggactgtggt
      541 gaagatataa aaggtagagt agtcacttta tcatttggaa tggagactct aatagagact
      601 ttaaaagaag gagacatagc tattgttgga gatagatatg atagtattga atatgctatt
      661 gatacaaaag ttaaactttt aatacttact aatcatacag agatttcaaa agatttgcta
      721 gctcttgcta agataaatgg agtgtctgtt gtttctgttg aaagtgacaa ttataaaact
      781 tctaatgtaa taaatcaatg tagtttctta gactctatag aagctacaga aaatttaatt
      841 acattcaaag aagatgatta tatggatgaa ataaaagaga ttatgctaga aacaaacttc
      901 agatcttatc caatattaga tgatgataat aagtttttag gtttagtttc aaaaggtcat
      961 ctattaaacc catctaaaaa gaatgttgtt ttagttgacc ataacgaata tgctcaaagt
     1021 gctgatggaa ttgaacaagc aaacatagtt gaaataattg accatcataa acttggtggt
     1081 atttctactg atgttcctat gtcatttaga gttatgcctg ttggatgcaa tagtactata
     1141 atttatcaaa tgtacaaaga aaacaatgtt gagatacctt atgaaatagc tgggcttctt
     1201 ttatctgcta tattatcaga tacattatta tttaagtctc caacaacaac tgatatggat
     1261 aagaaagctt gtgaagaatt aagtaaaata gcaaaagtag acatggaaaa atacgctatg
     1321 gatatgttta aatgcggtac ttctttagat gaatatacta tagaagaaat agtaaatatg
     1381 gattttaaag aatttaatat gagtggacat agagttggta ttggacaagt atttacctta
     1441 gacatagact ctatatttgc taaaaaagat gagtttttat cttatataaa ttcgactgat
     1501 tatgacaaac taattcttgc ggttacagat ataatcaaag aaggttctta tttaatctat
     1561 aaagcagaag atagcttaat tgctaacgca tttgatgttg aaggtgttca aggtacattt
     1621 gcaccaggtg ttgtatcaag aaaaaaacaa ttagtcccta atttaactac tgcaatcaaa
     1681 aattttaaat aatataaaat ttttaaataa cgaaaactgt ctcaaatcta tatttgagac
     1741 agttttttat tatacatata ttgataataa agttaaataa aaatccataa atttaaaatt
     1801 tttatgaata tattttatta ttattgttat tttattcatt ctaaaatagg taaaactcaa
     1861 tatataaaaa tacttttgtc atttaaataa aacctaaata gtttttaaga ttattttaga
     1921 cacgttttaa gtcataacag tatcagctat tctaaatatc gtcaaagtag ccgcagttcc
     1981 tgctgattgc ccatttcgta tccctactgt tgtagatgaa aaattattta ttgtaaaagt
     2041 agtcccccca aataaaaaac cagcaaaata tccagaaagt actcctccgc ctcctggctc
     2101 agtagtaagc cctgccaaat cttttcctcc taaaattggg aataggatag caaaagaata
     2161 ttgatttata cttgatgaac ctggttgtaa tgctactgaa actgatatat aatacacacc
     2221 attttcttgt actgtaaacg taccatttgc atctactgtt atacttgaac tatttcctat
     2281 tattgtttac caacctgtta tagcatttcc attatttagt gtttgtggag atggtgcatt
     2341 tacgaggaag ttagcaattg gctgagagga attgcctgtt gctcctgttg gacctgttgc
     2401 tcccattggt cctatacttc ctgtcgctcc tcttggtccc attggacctg taattctaat
     2461 tggaagacac tcacatttag gataacaatt atctataatt ctatcttcaa atgttacaag
     2521 tatatacggt ttgttacaat ccctactaga tgtaaattca aaaatctgat aagggtaatt
     2581 taaagataaa gctaacccat aattaggata tttattcatt gcccatgctt ttatagttcc
     2641 cgtaatattt aaacaaatat agttggatgt atcacttatc ccaattggta aatatcccct
     2701 gatattttcc atagacactc tatcattcca tgttatagtt cgtaaattat aatattcact
     2761 tattctttta atttcaatat ctactttttc tttaaaaata tgattcttat ttgaatcaat
     2821 atgaataaat aactctgctt ttaatatact tttgtctttt aatatatctg gtattctcac
     2881 ttaaaaaaaa gtaatcaact tttcttttac aaaactattt gaacatttac ttcctattaa
     2941 gcaataatct aaattactaa agtttttatc tggatatttt ttagatataa aagtatcatc
     3001 atttaaataa agtataattt ttctcattta taatccctcc attttgggtc tatatcatac
     3061 tatgtttaaa atacatgctt agttacaata caattaaaat ttatagtatt aaatttttat
     3121 ataataatgc tgatttctac aaaaaaagaa catgtcatta gaaacttcta atagcatgtt
     3181 ctttttcatt attatatatt acactttaaa ttattccttg actggcttag ctggtgtaaa
     3241 ctaaaccagt ctttctaaaa tattaagcac ttttttctta ttatgttttt taggatttat
     3301 tgtctctatt attgtaaacc aaagttataa a
//
LOCUS       DAGQWR010000243         3319 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.247, whole genome shotgun sequence.
ACCESSION   DAGQWR010000243 DAGQWR010000000
VERSION     DAGQWR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.247"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            524..3166
                     /gene="adhE"
                     /locus_tag="MIW95_003790"
                     /gene_synonym="adhC"
     CDS             524..3166
                     /gene="adhE"
                     /locus_tag="MIW95_003790"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBY3153081.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPDRVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGNTDQEKVDNLIKAIDELKARVGLPKTIKEAGVEESKFLETLDVMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         1284 a          422 c          632 g          981 t
ORIGIN      
        1 ctatatgaaa agcataatta ttacatattg acttaaaaat aacttataga gattagaata
       61 attttagttt gtatattttt taaaaccata cagttgttat aaaaagaaaa attgcacaca
      121 agtcaaaaat aagtgttatt ttataaaaat acgacaaaaa ttttatttac tacttataca
      181 aataacatgc aaataacata taaaatttaa taagtaaaaa aaatgaaaga tataaattat
      241 cattttagta ttttatttag ttgaagtaaa ttattcaaat gaaatatatc tcatttagat
      301 aaaaaatttt agtaaaagta aaaaaaataa tatatttgtt aaaaaataaa ttgacaaaat
      361 attcagaaaa aaacttcttg acctaagaga gtaaaataaa taaaatctaa ggtatagaga
      421 tgaaatctat aaaattattt taagtataag ttaaaaatat agatggtgca tttaagattt
      481 ataaaaatta gagatgcata attattttag ggaggttttt agtatggaga aaaaagaaaa
      541 ggtagtagaa aaatcaaatg ttgaagtatg tagtcctgaa attgtcaaca gtgttgaaac
      601 attaagaatg agattagaag aaattagatt ggcacaaaaa gaatttgcaa catttacgca
      661 agaacaggta gataagattt tcttagctgc ttcaactgca gctaatcaac aaagaatacc
      721 tctagcaaaa atggcagtgg aagagactgg tatgggtatt gttgaagata aggttataaa
      781 aaaccatttt gcctcagaat atatctataa tgcttataaa gatactaaaa cttgtggtgt
      841 catagaaaaa gatgaagcat ttggttttac aagaattgct gagccagtag gtgttttatc
      901 agcagttata ccaacaacta atcctacatc aactgcaata ttcaaatccc taatagcact
      961 aaaaactaga aatggtataa ttttctctcc tcatccaaga gctaaaaact gtactataga
     1021 agcagctaga gttgtacatg atgcggcagt taaggcagga gcaccaaaag gacttatagg
     1081 ttgggtagat gttccttcga ttgaattaac taatgttgtc atggcagagg cagaccttat
     1141 attagctact ggtggacctg gaatggttaa atctgcatac tcttcaggta aaccagctgt
     1201 tggagttggg cctggaaatg tacctgcaat aatagatgag agtgcagata taaaaatggc
     1261 tgtaagttca atcttagtat caaaatcatt cgataatggt atgatatgtg cttccgagca
     1321 agctgtaata gtaccagaaa aaatatatga agaagttaaa aaagaattta aataccgtgg
     1381 agcacatttc ctaaataaag aagaaactga aaaagtagga aaagtagtaa taattgatgg
     1441 ttcattaaat gccagaatag ttggacaacc agcacatgta atagccaaaa tggcggatgt
     1501 agaagttcca aaaacagcta gaataataat tggtgaagta gaatctgttg aattaaatga
     1561 accttttgca catgaaaaac tttctcctgt acttgcaatg tacaaatcta aaagttttga
     1621 agatgcagtt gctaaagctg aaaaattagt tgcagatggt ggatatggcc atacatcttc
     1681 gttatatgca gactctataa atcatccaga tagagtagag aagtttgtaa atgctatgaa
     1741 aacttgtaga gtattagtta atactccatc gtcacaaggt ggtataggtg acttatataa
     1801 ctttaaatta gcaccatctc ttactcttgg ttgtggttct tggggtggaa actcagtatc
     1861 tgaaaacgtg ggagtgaaac atttacttaa tatcaagaca gtagctgaaa ggagagagaa
     1921 tatgctttgg tttagagcac cagaaaaggt ttatttcaaa aaaggttgtt taggtgtagc
     1981 agcaagagaa tttaaagatg ttatggataa aaagaaagcc tttatagtaa cagattcttt
     2041 cctttacaat aatggataca ctaagaagct aactgatttg ttggatgaga tgggaattaa
     2101 gcatactaca ttctttgatg ttgctccaga cccaacatta gcttgtgcaa gagaaggagc
     2161 taaggctatg gctgatttcc aaccagattt aataatagct gtaggtggag gtagcgcaat
     2221 ggatgctggt aagataatgt gggtaatgta tgagcatcca gaggtagact tccaagattt
     2281 ggctatgaga tttatggata taagaaaaag agtatatgta tttcctaaga tgggtgaaaa
     2341 agcgtatttt gctgctatcc caacttcagc aggaactggt tcagaagtaa ctccatttgc
     2401 agttataact gaccaagatt caggtgtaaa gtacccatta gcagattatg aattaatgcc
     2461 aaacatggct ataattgatg ctgatatgat gatggagatg ccaccaagat taacagcagc
     2521 atcaggtgtt gatgctttaa cacatgcatt agaagcatat gtatctatgt taagaactga
     2581 accagctgac ggtctagcac tacaagcagg taaaataata tttgaatatc tacctcgtgc
     2641 atacaaaaat ggaaagaatg ataaagaagc aagagaaaaa atggcaatgg catctactat
     2701 ggcaggaatg tcttttgcaa atgccttctt aggaatatgt cactctttag cacataaatt
     2761 aggtgcattc caccatgttc aacacggtgt tgcaaatgca ttattaataa atgaagtaat
     2821 taagttcaat tgtgcagaag ctccaaataa gatgggtgca ttctcacaat acagatatcc
     2881 tgattgtata caaagatatg ctgaatttgc ttcttttgca ggaatcaagg gaaatacaga
     2941 ccaagaaaaa gtagataacc ttataaaagc aatagatgaa ttaaaggcaa gagtaggact
     3001 tcctaaaact ataaaagagg ctggagtaga agaatctaaa ttcttagaaa cattggatgt
     3061 tatggttgaa caagcttttg atgaccaatg tacaggtgct aatcctagat acccattgat
     3121 gagtgaatta aaagagatat atctaaaagt ttattatggt aaataatatt cattgtacaa
     3181 cataggttct attgggtttg tatataaatt attagatttg aatataaata gagttttatt
     3241 agagaaacgt agaagtaact ataaataata gattataact taaattctga atggaaatga
     3301 taaaaagtca gtaatttaa
//
LOCUS       DAGQWR010000244         3313 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.248, whole genome shotgun sequence.
ACCESSION   DAGQWR010000244 DAGQWR010000000
VERSION     DAGQWR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.248"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(180..563)
                     /locus_tag="MIW95_003791"
                     /pseudo
     CDS             complement(180..563)
                     /locus_tag="MIW95_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      790..887
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            942..1118
                     /locus_tag="MIW95_003792"
     CDS             942..1118
                     /locus_tag="MIW95_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153082.1"
                     /translation="MFGNKMDKCTHVLTAYISGSYDYCNFLDTQLDDFILEYGESVVE
                     SCLHQVMVLVSKYN"
     gene            1344..1544
                     /locus_tag="MIW95_003793"
     CDS             1344..1544
                     /locus_tag="MIW95_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBY3153083.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(1708..2007)
                     /locus_tag="MIW95_003794"
     CDS             complement(1708..2007)
                     /locus_tag="MIW95_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153084.1"
                     /translation="MGFYINYVVCKVKILFSSFSKIILFYINYVVCKLFITLKAILLK
                     TQIIVTTHSPHILQIDSKEEMIVLDMAESGNVYKKELELGEYGVLGWTNEGLYLH"
     gene            2229..2411
                     /locus_tag="MIW95_003795"
     CDS             2229..2411
                     /locus_tag="MIW95_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBY3153085.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            2482..>3313
                     /locus_tag="MIW95_003796"
     CDS             2482..>3313
                     /locus_tag="MIW95_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153086.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINNSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     K"
BASE COUNT         1244 a          398 c          422 g         1249 t
ORIGIN      
        1 tatatatggc tatttttcaa cattttacaa tatatataac atttattttt ttaacttttg
       61 tcagtaaaat gtcagcaaga cactttatcc acaattttta tataagtttt ccacaactta
      121 tccccagtat tatccacata aaaaaggtag ctcttatatt attagctacc ttttatttat
      181 taatcttcat atttccagct ttttatccaa cttcctattt ctattatttt ttcttcagtt
      241 atttcttctc catcatgtaa ttttgaaatt aattctgata aaggattgtt atgtaaacct
      301 ctaaaaactc tttttccctc tattttgaga tattctctct cttttattaa tactgtatag
      361 tgtacatgcc tcccaattct ctatgatatt aaaaaacctt ttttggttaa tctagctaaa
      421 agtataattg tagttgtatg cttccaacca tatgtttttt ctatttcatc tgcaacatct
      481 tttgatatta cagttttata accagtattc catatgcatt tcataatttt taattctgta
      541 tctggtaatt ttttatttag cataatatat tccttctttc aatttatgtc acttcttcaa
      601 atttatctag cttaaattat aataggagtt ttttagaaca aaaactatag ttaattttta
      661 ccaggtactc tacgtgttta gtctgttttt taagtgtaaa ataatgttca tatctataaa
      721 tttatttaac tttcaaacaa tcctgttgaa taataactaa ttctattgta ttattttagt
      781 tgtagaataa aactaaatcg gcaaaactag agaaatttag tgacgcaaag ctatagggac
      841 taagacttat ataaatatct tatgagttat gtcagccagt tgccaaagag atagtgttct
      901 ttttgttttt atgaaagttt tttaggggga taataattta tatgtttgga aataaaatgg
      961 ataaatgtac acatgtattg actgcttata ttagcggttc gtatgattat tgtaattttt
     1021 tagatacaca attagatgat tttatattag agtacggaga aagtgtagta gagtcttgtt
     1081 tgcaccaagt gatggtattg gtaagtaagt ataattagat agacattgat ttctagagaa
     1141 ccctttttag ttttatctac atttatatta atatatagca atgtaaaacg cttaaatata
     1201 tttttaatat taagaataac taaaatgttg actaaaaaat ataattaagt agacatttct
     1261 attaatctat gatacaatag tatttgtggt aatacacaat taaatcaagt atatcttgat
     1321 ataggttaat aggagtattt ttaatgaata atggaatagt gaaatggttt aataatgaaa
     1381 aaggattcgg ttttatatca atggaaggta gagatgatgt gtttgctcat ttctcagcta
     1441 tacaaacttc aggatttaaa tcattagaag aaggtcaaca agtaagcttt gatatagtta
     1501 aaggtgctag aggtcctcaa gcagaaaata taactatatt ataatatata gatttttcgg
     1561 gaaagactct ttttagagtc tttttttatt gttagtatat gtaagataat cattggtgaa
     1621 gtaaataagg tatattgagt aatgttcttg atagtttata tgctaatgaa tttcaaaaaa
     1681 ataagcactc ctataaaaag agtgctctta atgtaaatat aagccttcat tggtccagcc
     1741 taataccccg tattctccaa gttctaactc ttttttatat acattaccac tttcagccat
     1801 atctaacaca atcatttctt ctttagaatc aatttgtaaa atatgaggac tgtgtgttgt
     1861 gacaattatt tgtgttttta ggagaattgc ttttaatgta ataaaaagtt tacatacaac
     1921 atagttaata taaaacaaaa ttatttttga gaatgagcta aataaaatct taactttaca
     1981 taccacatag ttgatataaa accccaaaat aaacttagca tctccaatgt ttacacatat
     2041 acaatgctct taaaattgca gtgaacatgc aatagtgcaa ttgataatac ttatcatata
     2101 ttctcaatac cttatatttc aactgctaaa cctcaatttg tcacaaatat cgctcactgc
     2161 aaaatttcta tatttttatt atatcatagc atataattaa tattttcaat taatttttac
     2221 atttaattat gtttaaattc tataataatg caactttaat gtttctttct ggaactaata
     2281 taaaaactat ttcagaaaga ttaggtcatg cagatataaa aataactatg aatagctact
     2341 ctcagttttt agaagagatg gacagagaag catttgaaaa tttgagcaaa atactattta
     2401 ttgtatttta aagaagatga cttattcaat atttgatgct agaatataat taataaaaat
     2461 ttaattatag gtgattgtat tatgaacatt attaaaagca taactttact cttaacatta
     2521 ttaattgtga tattaatgac aggatgttcc ccaagagaaa gtgctagtga agttgcaaaa
     2581 aaatatattg ataagatgaa ctctggtgac tatgaaacag catatagttt attaagtaaa
     2641 gatagtaaaa aaaaaattag tatagaaaat cttgaagaat ttcaaaatat tttagttagt
     2701 acaaaaagaa taattagtta taaaataggt aaagaaaaaa tcataaaaaa atataaacat
     2761 gatggaaaag aatataaaaa tgttgttaaa ttagaagaaa cttttaatat aaaaaacctt
     2821 ctttataaaa aagatgattc tctaaaagta aatagatatt ttgtagttga aaataacaaa
     2881 tacaagctat tattgtatga aagtttcaaa aaagaatttg gtattaatag tatggacctt
     2941 gctcaactta agcttaatga ttttggacta aaatacttta aagaagttga ccttatacta
     3001 aaaaaagcta ttgctattaa tcctactgat tcaaagcttt attatgccca agcatgtaat
     3061 tatatgaatt ttgatggatt tcctggttat tcagatacaa catataatgc tttagattca
     3121 attaataatt ctttaaagta tttagataaa aatgattctg actacaaaaa aaatgcaagt
     3181 aatatatata acttaaaagg agttattctt atgtttcaca aaagatttga tgaagcacaa
     3241 caatatttaa ataaagcctt atactttaac aaagataatg aagacccaaa atataatcta
     3301 aatacaataa aaa
//
LOCUS       DAGQWR010000245         3239 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.249, whole genome shotgun sequence.
ACCESSION   DAGQWR010000245 DAGQWR010000000
VERSION     DAGQWR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.249"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(328..504)
                     /locus_tag="MIW95_003797"
     CDS             complement(328..504)
                     /locus_tag="MIW95_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153087.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(558..654)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1450..1851
                     /locus_tag="MIW95_003798"
     CDS             1450..1851
                     /locus_tag="MIW95_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBY3153088.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            1848..2915
                     /gene="rsgA"
                     /locus_tag="MIW95_003799"
     CDS             1848..2915
                     /gene="rsgA"
                     /locus_tag="MIW95_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBY3153089.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKSGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
BASE COUNT         1158 a          400 c          536 g         1145 t
ORIGIN      
        1 agttataaaa gaataagtta tataaaaaat tattatattt tttaatatag cttatttgca
       61 aatttataat tttgttaatc taatccttca aactaaaaaa tcatttatgt ttacagaatt
      121 tcattttaag agatgtttat tttcacatag ataagagaat ttaagttctc atatttatta
      181 tttgatacaa atttacatct ttattatatg tattttaaat agaaaataaa attatgaata
      241 ctagaaatct atcttaatta gctttctaac ataaggctat ttaaagtaga taaaactaaa
      301 aagggttctc tagaaaacca aatctattta attatactta cttactaata ccatcacttg
      361 atgcaaacaa gattctacca cattttctcc gtactctagt ataaaatcat ctagttgtgt
      421 atctataaaa tcacaataat catatgaact accaatataa gcagtcaaca tatgtgtaca
      481 tctatccatt ttatctctaa acatatatat tattatcccc ctaaaaaact ttcataaaaa
      541 caaaaagaat atctcttttt ggcaactggc tgacataact catagtttat tttataagcg
      601 ttagtcccta tagctttgcg tcactaaatt tctctagttt tgccgattta gttttattct
      661 acaattacaa taatacaata gaattgctta ttattcaata tacttatttg aaaattaaat
      721 aaatttacaa atatgaacgt tattttatat aaaaaaaata gactattcat gtagaatata
      781 tggaaaaact tgattgtagg ttttgtatga aaagacagtt gttattaatt taagtgagat
      841 aaattgagca ataggcataa aatgagaaaa caaataatag caattaattt ttattattaa
      901 tactaggtga acattaaatt ttaaaatacc actagatttt atagaataag aaaatgagac
      961 aaacataaat atatgaatat attaaattat acttgaacat acttatattt tatgataaaa
     1021 ttttaataat aattattagt gaaggagtgt cttgcctgat gtcagagtta tatataatgg
     1081 taaccaacaa ataaatagtt ggagacgcat atgggttctg tttaatcaga atttttaagt
     1141 gtatttttga tacatttgat tgttgtgtct tactctgcct ttatagataa ctaacattag
     1201 acaacaaata ataatattac atattacttt gttaaattgt ggtgtagtgg gctttaatct
     1261 gctacacttt tattaatttt acacagtatt attttgaata taattatgtt gaaatagaag
     1321 atactaaatt ttctagtcca caggggtttt ctgtttttgt gtaggtaaat atgtatacca
     1381 cataaaaagg cagatataaa tacctgtgga ctttttttgt agaaaataca agatacggag
     1441 gaaataaaaa tgaattataa aaatagaaaa cgagcttatt atcaggaaaa ttcctgtatg
     1501 gataacttta tttgcaaaaa ttgtggttgc cttgtagttc ctgaaggagc tggtacacaa
     1561 catagaaatc attgtccaca ttgtttacat agtcttcatg tagatatcat tcctggtgac
     1621 cgtgctgcag attgtgatgc taatatggaa cctattggag tgtgggtgag aaaaaatggt
     1681 gagtgggcaa ttattcatcg ttgtacacgc tgtggacatc ttagttctaa tcgtattgca
     1741 gctgatgata attcagtgaa attaatgtca attgcattaa aaccactggc acagccccct
     1801 ttcccactag aaagaatcat tgaaaagttg gaaaaggagg aaacagaatg aatggtatga
     1861 aacaaatcaa tttacaagac tatggtctaa ataggaattt tattactcaa agtatggaat
     1921 atcacgattt ataccttgct cgaattatct cgcaggaaaa gaacttttat caagctgtat
     1981 gtgtaaatgg tgtactgttt gcagaggttt caggaaaact gcgttttgag gctactatac
     2041 aatctgattt tcccacagtt ggtgatttcg ttatgttgga tcgttgcaat aataaaaatg
     2101 gtaatgctat tattcatcat gtgcttcata gaaaaagctt gtttgtgcgt aaggctgctg
     2161 gaaaggtaaa tgaggaacag gtggttgcag ctaatattga cattgttttt ttgtgcatgt
     2221 cactgaataa ggattttaac attcatagat tggagaggta tctgtcttta acatgggaaa
     2281 gtggagctat cccagtagta gtacttacta aatcagactt atgtgacaat gtgcatcgta
     2341 aattacaagt agttaattct atagccttgg gtgtagatgt agtagttacc tcatctatag
     2401 agccagatgg ttataaacag atttctaagt atatcaaatc aggctgtact gttgcattac
     2461 ttggctcttc aggagttgga aaatcttcat taattaattg tctgctagga ggagaatttt
     2521 taaatacaaa ggaaattcgc atagatgatg ataaaggaag acatgccacg actagacgag
     2581 agctattttt aatttcaggt ggtggtattg ttattgacac accaggtatg agagagttgg
     2641 gaatgtggga tgtttcagaa ggtttagata aaagttttgc agatatagaa gcttatttcg
     2701 gaaaatgtca ttttcgtaat tgtactcaca cctcagaacc aggctgtgct atctgggaag
     2761 ctattgctca tcaagaactt tcgcctaaaa gatgggaatc ctatcgtagg cttaaaactg
     2821 aagcagtatt tgcagacaat aaaagcgaat acttagcaaa aaaagaaaag aaatttaaaa
     2881 atatctcaaa aataaataag acgattagaa gataagtgta gtttttttgt tcattctatg
     2941 agtctatata ctattaatgt attaaatttg ccattaattt gttactttaa tgtgattttg
     3001 atggcaaatt tggtttgtgg tattatttaa agcaatttat taattttttt aataaagttt
     3061 gattttttat atgatgatat atatatgtga taatgtatac atatttgtta cgatagtctc
     3121 ctttataaag aggtcttatg taagataaga aaaggcatat ttatactaat acataataga
     3181 gtatatgctt atgtatgata gagatgtttc ttaagagcaa aactgcaata ttgctaatt
//
LOCUS       DAGQWR010000246         3182 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.251, whole genome shotgun sequence.
ACCESSION   DAGQWR010000246 DAGQWR010000000
VERSION     DAGQWR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.251"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     regulatory      189..232
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            333..1301
                     /locus_tag="MIW95_003800"
     CDS             333..1301
                     /locus_tag="MIW95_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBY3153090.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            1303..1866
                     /locus_tag="MIW95_003801"
     CDS             1303..1866
                     /locus_tag="MIW95_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBY3153091.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            1874..2563
                     /locus_tag="MIW95_003802"
     CDS             1874..2563
                     /locus_tag="MIW95_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY3153092.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLRVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMIGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            2690..>3182
                     /locus_tag="MIW95_003803"
     CDS             2690..>3182
                     /locus_tag="MIW95_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBY3153093.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIKDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSGLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEI"
BASE COUNT         1232 a          317 c          578 g         1055 t
ORIGIN      
        1 atgcgccaca tcttattaaa gttggttcat aattctaaat aaactttaat aagatacatt
       61 aaaatataat aaattctttt atgctataaa atttatttta tagctattaa atcagatgtt
      121 taaaaatttt ttaacagtct atagttttta agagtctata gtaagtagcc taaaaatatg
      181 attttacatc tgtacttgtt cgagaactcc aagtataaaa aactaaggaa ttatatagtg
      241 gcagtatata attaataccc ttagacacac ctttttgaaa gtggaaaaaa gatgtgtagt
      301 tctcctcgac cacaaatcaa ggaggataaa aaatggaaaa gagaaaagta ataattgatt
      361 gtgacccagg aattgatgat tctttggcaa ttcttctggc tttaaactca ccagagctag
      421 aagtaattgg aattaccaca tgttgtggaa atgttccagc aaatataggt gcagaaaatg
      481 cactaaaaac acttcaaatg tgttcttcac taaatattcc agtatatata ggagaagaag
      541 caccactaaa aagaaaactt gtaacagctc aagatacaca tggagaagat ggtattggag
      601 aaaactttta tcaaaaggtt gtaggagcta aagcaaaaaa tggagcagtg gattttataa
      661 taaatacttt acataatcat gaaaaagtat caataatagc acttgcacca cttacaaata
      721 tagctaaagc acttattaaa gataagaaag catttgaaaa tctcgatgag tttgtatcta
      781 tgggaggagc atttaggatt catggaaatt gctctccagt agcagagttt aattattggg
      841 tagacccaca tggagcagat tatgtttaca agaatttatc taaaaaaatc cacatggtag
      901 gtttagatgt aactagaaaa attgtactta ctcctaatat tattgagttt ataaatagac
      961 ttgataagaa gatggcaaag tatataactg aaataactag attttatatt gatttccatt
     1021 gggaacagga aggaataatt ggctgtgtga taaatgaccc tctagcagta gcgtacttta
     1081 tagacagaag tatatgtaaa ggatttgaat catatgtaga agttgtagaa gatggaatag
     1141 ctatgggtca gtctatagtg gattctttca atttctataa aaaaaatcct aatgcaattg
     1201 ttctaaatga agttgatgag aagaaattta tgtacatgtt tttaaagagg ctttttaaag
     1261 gttatgaaga cattatagac tctgtggaag gagtgatata gtgtgaaaag aaaaatatgt
     1321 gtaaaaacta ttacacttat agcttttggt atttctataa atataatagg cgcttttata
     1381 gctatgggat taagattacc tgtatactta gattcaatag gaactataat gatagcttca
     1441 cttttaggac ctaaatacgc tgttgttaca ggtgtgtttg gaagtttaat aagtggaatt
     1501 acgtttgatg tgtattctct ctattttgca ccagttcaaa tatcaaccgg attacttgct
     1561 ggaatagcat ttaaaaagaa ttttttaagg ggtttaaaaa ctccactagg agtactttta
     1621 tttgctattc caacatctat aataagttca ataatttcag catttttatt tggaggtatg
     1681 acctcttcag gttcatccta tatagtgcaa atcttgaaag tgttaggact gggagatgtt
     1741 tttagtgtgt ttgtaaccca agtatttact gattatggag ataagctgtt agcagttgtt
     1801 ttagtgaatt taggattaaa tgcagttcca aaaactttaa aggtatcatt gactggagga
     1861 aaatagatag aaaatggata gatatagtga gataacaaat aaaaatcaaa gagaaattgt
     1921 tctgcttaaa ggatttcctt gtatatgggg aaagtgttct ttttgtgatt atatagatga
     1981 taattctaat ttagaagaag agatgaataa attaaatcta agggttttaa aaaatgtaac
     2041 tgggaaatat ggagttcttg aagtcataaa ttctggaagc tgttttgaat tacctaagga
     2101 tactttagaa aaaataaagt gtattataaa agagaagaat ataaaaaaat tatttttaga
     2161 aagtcattgg tcttacaaaa ataggcttaa ggaaatgaga gagtattttg aaattcctgt
     2221 ggtatttaaa attggagttg aaacctttga caatgatttt agaaataata ttttaaataa
     2281 aaatgcaaat tttaagactc cacaagatgt aaaagaatat tttgattcac cttgtattat
     2341 gattggaata aaaggacaaa caaaagagat gatagataaa gatatagaga ttatcctaaa
     2401 tacttttgaa aaagcgacag tgaatgtttt tataaataat tcttctagta taaaaagaga
     2461 tgaagagtta gtgaaatggt ttgttagtaa gtataaattc ttggatgaaa atccaaatat
     2521 agaagtttta tacaataata cagactttgg tgtaggagat tagcattaat ttagtttaaa
     2581 tttaaaattg aaaagtttct tatgtttttg aaaaagcttc taatagttag aagctttttt
     2641 tgtagagttt tatgttacaa taagaaaaaa ggaattaagg agcaattaaa tgatgtttga
     2701 taggggaaaa tggaataaat tggattataa tgaactttta gaatatatga tgtctataaa
     2761 agatataaaa tatagagatt ttaataaaaa gcttatacct ggaacagaaa atataatagg
     2821 tattagagtt cctaatttaa gaaagttatc aaaagaaata agccaaggga attggaagga
     2881 atttttagaa gtagcagagg atacatacta tgaagaagtt aggctacaag gtatggtaat
     2941 tggcaatata aatagtaatt ttgaagaaac tttatactat gtaaaaagat ttatacctaa
     3001 aatcgataat tggtcagttt gcgatgggtt ttgttctggt ttaaagtcta tgaaaaaata
     3061 taaagaaaat atgtatgata tacttaaaaa atatgtttat tcaaagaatc catgggaaat
     3121 tagatttgca ttagttatgt ttttaatata ttatgttgat gataaacata ttaaagaaat
     3181 at
//
LOCUS       DAGQWR010000247         3015 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.252, whole genome shotgun sequence.
ACCESSION   DAGQWR010000247 DAGQWR010000000
VERSION     DAGQWR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.252"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..1512
                     /locus_tag="MIW95_003804"
     CDS             <1..1512
                     /locus_tag="MIW95_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBY3153094.1"
                     /translation="KRFEQLEEVQNNKSVKYYLELEKKYKLAMNSIEKDILSWYNRFA
                     ENEGISLLEAKKLLNTRELEEFKWNVEEYIKYGKENAINQKWMKELENASARLHITRL
                     EALKLQIQQHVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWSLMSLDTNR
                     INKIISKPWATDGLNFSERIWGKHRPTLINELHTKLTQSIIRGENPKNLVNDFAKRFN
                     VSKSQAKNLIMTESAFFASASRKDCFSDLDVEKYEIIATLDLRTSNICRELDGKIFDM
                     KDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYNEWYEKHIE
                     GRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYNDTIRYEEAK
                     KLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGNTGKPRLDL
                     DLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDILNGE"
     gene            1514..1762
                     /locus_tag="MIW95_003805"
     CDS             1514..1762
                     /locus_tag="MIW95_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153095.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            1830..2393
                     /locus_tag="MIW95_003806"
     CDS             1830..2393
                     /locus_tag="MIW95_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBY3153096.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTVTQEDYVNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDMEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            2405..>3015
                     /locus_tag="MIW95_003807"
     CDS             2405..>3015
                     /locus_tag="MIW95_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBY3153097.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATENNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDGGTSLNRQTT"
BASE COUNT         1306 a          328 c          554 g          827 t
ORIGIN      
        1 aaaagatttg aacaattaga agaagttcaa aacaataaaa gtgtaaaata ttatcttgaa
       61 ttggaaaaga aatacaaatt agctatgaat agtatagaaa aagatatatt atcatggtat
      121 aacagatttg ctgaaaatga aggaatatct ttattagaag ctaagaaact gttaaataca
      181 agagaattag aagaatttaa gtggaatgta gaagagtata ttaaatatgg caaagaaaat
      241 gctataaatc aaaagtggat gaaggagtta gaaaatgcta gtgcaagact tcatataaca
      301 aggcttgaag ctttaaagtt acaaatacag caacacgtag aagttttata tggaaatgaa
      361 cttgatggta ttgataaact aatgagagat atttatacaa gtggatacta tcatacagct
      421 tttaatgttc aacaaggagt aaacgttggt tggagtttaa tgagtcttga tactaataga
      481 ataaataaaa ttatctctaa accatgggca acagatggat taaactttag tgaaagaatt
      541 tggggtaagc atagacctac tttaataaat gaactacata ctaagctaac tcaatcaatt
      601 attagaggtg aaaatccaaa aaatctagta aatgactttg ctaagagatt taatgtatct
      661 aaatcacaag ctaagaattt gataatgact gaatcagctt tctttgcatc agcaagtaga
      721 aaagattgtt ttagtgattt agatgtagag aaatatgaga ttattgctac attagattta
      781 agaacttcaa atatatgcag agagttagat ggaaaaatat ttgatatgaa agattatcaa
      841 gttggaataa cagctccacc atttcattgt cgttgtagga caacaacagc tccttggttc
      901 gaggatgaag aaggctatag agcagcaaga ggagaagatg gaaaaacata ttatgtacca
      961 tctagtatga agtataatga gtggtatgag aaacatatag agggtagact tggaaaagaa
     1021 aaagctgaca cccttagaaa aatgcaacta aatgagagta aagatagaaa acaatttgaa
     1081 gaatataaaa aggtattagg aaatgaaata ccttctaaat ttgatgaata tcaacatatg
     1141 aagtataatg atactataag gtatgaggaa gctaaaaagc tttataagga tgtaaattgg
     1201 caagttaaga atcagagaaa tttaactagt ggaagtgttc attcagtgcc atttgaatca
     1261 aaaccaaata gtgtatttga taattacaaa gatggtaaat taattcaaag aagatactat
     1321 ggtaatacag gaaaacctag attagattta gacttaactg accatagaaa cccaaagcaa
     1381 cataaaattg tacctcataa acatgattgg ttagctgatg aaaataacca tgataaggta
     1441 aaaagagaga aggatatgga gcttacaaaa gctcataaaa tagctaacaa agatattttg
     1501 aatggagaat aaaatggata ataaatttaa gaatttacaa catttaattg attctattga
     1561 tttaggatta gatatagaat ttgacttata taataaacca tataatatat ctattggaga
     1621 tgatgataca aggtttatta ctctttgccc taatggagat acaaagtatt ataaaaatgg
     1681 taaggatatg gttgataatt acaacattga tggtaaatta ctaaaggatt tatggaaaga
     1741 tatacaaata gtaaatatgt aaaagcactt gctaaataat aaattagtag gtgcttttat
     1801 tttgtaaaaa atgaaaggag aaatttaaaa tggattggtt aaaagaattg ctagaaggaa
     1861 taaaagtaga ggataacaaa attgatgtag cttctcttca aaaatctata gaaaagaaaa
     1921 taaaggagac tacagttact caagaagatt atgtaaatct tgaaacacaa cttaatacag
     1981 ctaatgaaac tattaaaaag tttgaaggag gtatgacaaa agaagatgta gagaatctaa
     2041 aaacaactta tgaaactgat aagaaaactt tggaagaaac ctacaaaaaa gaaattgaag
     2101 aaaaagattt taattactgg ttaggtgatg cttttaaatc tgttaaatgt agagatgaaa
     2161 tagcgttaaa agctcattta gacatggaag ccctaagaaa tagtaaagat agacaaaaag
     2221 cttttgaaga gcaaataaac cctttgaaac aggataaaga ttatttgttt aatgcaacac
     2281 tagaaggtga agaacctaaa atagatacta taacaccagg gcaagagcct aagataaatg
     2341 attttggttt taattttact ggggtaagac ctcatgaaaa taataataaa taggaggaaa
     2401 taaaatggca gcactaaatt atgcaaaaga atattcaaat gttttagcac aagcatatcc
     2461 ttatacttta aacttcgggg atttgtatgc aacagaaaat aatggaagat atagatggac
     2521 gggttctaaa acaatagaaa taccaactat atctacaact ggaagggtag attcaaacag
     2581 agatacaata gcagtagctc aaagaaacta tgataatgct tgggaaccta aggtattaac
     2641 taatcaaagg aaatggtcaa cattggttca tccagcagat ataaaccaaa ctaattatgt
     2701 ggcttcaata ggtaatataa caaaagtata taatgaggaa caaaagtttc cagagatgga
     2761 tgcttactgt atatctaaaa tatatgctga ttggaccgca ttaggtaaca cagcagatac
     2821 aactgttctt acaacagcaa acgtattaga agtatttgat aagttaatgg aaaaaatgac
     2881 agaagctaga gtacctgaaa atggaagaat attgtatgtt actccagtag taaatacact
     2941 tatcaaaaat gcaaaagaga tacaaagaac agtaaacata aaagatggtg gaacttcttt
     3001 aaatagacaa accac
//
LOCUS       DAGQWR010000248         2953 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.253, whole genome shotgun sequence.
ACCESSION   DAGQWR010000248 DAGQWR010000000
VERSION     DAGQWR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.253"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            97..1242
                     /locus_tag="MIW95_003808"
     CDS             97..1242
                     /locus_tag="MIW95_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY3153098.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     SRGNRSGNTISIITKKELDIIKDLQKEFSIVITPKTLQNGELIDIIK"
     gene            complement(1356..2732)
                     /locus_tag="MIW95_003809"
     CDS             complement(1356..2732)
                     /locus_tag="MIW95_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBY3153099.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCLGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFM
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     regulatory      complement(2826..2928)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         1194 a          477 c          382 g          900 t
ORIGIN      
        1 ttacacataa ttataaaaga atatatagtt cgatttgaat aaacaatgta taataaaaaa
       61 tagttgacta taattattaa atataaggag caaaatatga atacatttga acaattaaaa
      121 ataagttcca ctctaataga tggacttaaa aaacaagaca taacttctcc aacagaagtt
      181 cagtcacttg ttatagggaa tataattcaa aataaggatt tattgattaa ttcacagact
      241 ggaacaggaa aaacactagc gtacctttta cctatatttg aaaaaattga tacatcaaaa
      301 agagaaacac aagctcttat acttgcacct acgcatgaat tagtaatgca gattacaaac
      361 caggttgaat tattggctaa aaatgcagaa ctctccgtta cttctttagc tttaattggg
      421 gaggtcaata tacaaaaaca aataaaaaat ataaaagcag taaaaccaca catagtaatt
      481 ggctcttgtg gaagagtttt agacttgata aaacaaaaga agctaaaatc acataatata
      541 aaaactatag tcttagatga agtggacaat ctattgaatg gaaaaaatat aacttgtata
      601 gaagacataa taagaacaac gttaagagat agacaaatta taggatgctc tgcaagtctt
      661 actgatagca ctataaaaat ttgtgataaa cttatgaagg agtttgaaat aataaaaaca
      721 aaggaaaaat cacaaataaa tcctaacatc aatcactcat atttattagg tgagataagg
      781 gataaattta cttttcttag aaaggcatta gcagctacaa atccaaaaaa agctattgta
      841 tttgtaaata atgaaaaaaa tatagaagtc ctagtatcaa aactaaatta tcataattac
      901 aaagcaatag gaatttttgg aaatatggaa aaagaagata gaaagaatgc aataaataaa
      961 tttaaacttg gtaaagcaaa gattttaatt acaactgatt tatctgctcg tggactagat
     1021 attgtggatg taagtcatgt ttttaacttg gattttccaa aaagtaaaaa tgaatacctg
     1081 catagatgtg gtcgaacttc tagaggaaat cgaagtggta acacaatctc aataataaca
     1141 aaaaaagaat tagatattat aaaagatttg caaaaagagt tcagtatagt aattacacct
     1201 aaaactttac aaaatggaga actaatagac attattaaat aatacaataa atacgaaaat
     1261 acaacaaata caaaagagct gtagtttatc atcacaattg ataataaatt atagcccttt
     1321 attttatatt aaattaaaat tttaaataat acctattata atagccaaag ttttattata
     1381 aataatattg ccagaacaat cattactaca gaaacatgtt ttttgtcttc tgccttttta
     1441 gctccaaata tattataaaa tatatttacg aaaacataag ttaatatacc taaagttaaa
     1501 ccatcaccta tactatatgt taatgccata caagcaatag taacaaaagc tggaacacct
     1561 tcagttatat tatcaaactc tatatcctta actgtaccaa gcattaaata tccaacatat
     1621 attaaagctg gtgctgtagc acatgatgga attgctataa atacaggaga gaaaaacatt
     1681 gcagctaaga ataaaattcc aactgttatt gctgtccatc cagttcttcc acctgcgata
     1741 actcctgttg aactctcaac ataagttgta actgtagata ctccaagtcc tgcaccaact
     1801 gtagttgcaa ccgcatctgt aagtaaggct ctacctacat taggaacatt ccctttttca
     1861 tctaacatgt ttgctttaga acaaacacca actaatgtac ctactgtatc aaaaaaatca
     1921 acaaataaaa atgtacaaac tactactaaa aaaccaccta tatttgttgg atgtagtaca
     1981 tatcctaaat ctattttacc tgctattggt gctaaacttt cgaatttaaa taatccacca
     2041 ggtaaatata tacctaattt ttgtgcatac tctggattca taaaagcaaa tgcccaagca
     2101 agtaatgaac tcactactat tccaaaaagt atagaacctt taactctttt tttatctaat
     2161 acagcaataa ttattaatcc taaacaagtt attattactg ctggtgtaaa tctacccatt
     2221 ttgataagag tggcttggtc tccaataaca attccactgt taactagacc tataagtgca
     2281 ataaatattc ctataccacc tgtaacagct aatttcatat tttttggtat cgcatttaca
     2341 acagcctcac gaactccact tactgataat actataaaaa taataccttc tacaaatact
     2401 gctgtaagag ctacttccca aggaacaccc atttgtaaaa ctactgtata tgcaaaaaat
     2461 gcattaagac ccatacctga tgctaaagca aaaggaaggt tagcaactac acccattaaa
     2521 atacatccaa atgctgctgc taaacaagta cctgtaacaa gtgcacctgc aggcatgcct
     2581 gtctcagata aaatatttgg atttacagct attatataag ccatagttaa aaaagtagtt
     2641 actcctgcaa ttatttcctt cttcatgtcg acatttttat tagtcaaaat tggaaaaagt
     2701 ttctgcatag tactactgtt tgttgtttgc ataacaacac ctcctattaa attttacaaa
     2761 aaaacgacac agtagttgat aagctgagcc gtatccttca taattcagtt aattaaatat
     2821 ttaattaact aaataatagt atacatagtc agaatattta cggtattccg gtagaaactt
     2881 atgacccata ttgtcaaaat tatatgttat acagtacaaa acatgaattt ttctttacga
     2941 atattatttt aat
//
LOCUS       DAGQWR010000249         2926 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.254, whole genome shotgun sequence.
ACCESSION   DAGQWR010000249 DAGQWR010000000
VERSION     DAGQWR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.254"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..387)
                     /locus_tag="MIW95_003810"
     CDS             complement(<1..387)
                     /locus_tag="MIW95_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3153100.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLAL"
     gene            complement(1196..2233)
                     /locus_tag="MIW95_003811"
     CDS             complement(1196..2233)
                     /locus_tag="MIW95_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY3153101.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKKTDSKKQ
                     KNIGISQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISTSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(2330..>2926)
                     /locus_tag="MIW95_003812"
     CDS             complement(2330..>2926)
                     /locus_tag="MIW95_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY3153102.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNNLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKENLTVEEILEKFEYAV"
BASE COUNT          992 a          445 c          381 g         1108 t
ORIGIN      
        1 taaggctaaa actataggag atatttcccc attgaataag tgtttaaagc taaatagagg
       61 aatatttgat tttcccatta ttcttaaatt gatagagtat agcattccca taactaaaat
      121 accagaaagt aagttagata tcttaaactt aatgtgaaga atccctgtaa caagaccagc
      181 agtacaacca cataagatag ccattaaagt tgctactaca ggagaaatac cattagtaag
      241 agaaaatgct acaatagaag cacccattgt atagctacca tcagcagaca tgtctggaaa
      301 atctaggatt ttgtaagtta tataaacccc cagtgccata atcgataaaa ttaaactttg
      361 cgtcattaca gatataatac ctgacataaa taaaacactc ctttgaaaat atttttataa
      421 ttttaattat ttgctaattt tgtgtataaa aaaaccacat agtgtacaaa actatgtggt
      481 aaaaacacga ataagccaca tagtttaatc tatgtggctc ttatatagtt aaatgaaaac
      541 tttataaaca atatagccaa catagataca atcccaaaat tttaatagtc ttaaggggtc
      601 tgtatctatg ttaaaacgta ggtacaaacc ctaaaaatag tggctataat aataataata
      661 ttttctattg tttaataaag tattcataat tatctctccc ttttaatatt ttaattagtc
      721 agaaaattat atgtattctc gacttgttat aaattataaa ttatacaata ataaaagtca
      781 actgctaaat ttaattaaaa tactaaataa tgtatactca gaatactttt aaattaatgt
      841 attgtttgca aaaaacgtta tcctaagtta taagtgaatg tggaaatttt atcaaaatat
      901 ttactaaata tggaataact agacatgtat atcaatcaaa tatcataaaa aaaagctgtc
      961 tggagaatat taaaattctt ttggacagct caagtagtga ttagaaagtt atataacttt
     1021 cataatcagt cgtcaatata gacgactttt atggaaaatg aataacttta tatgcaatat
     1081 tcaaattgct acaataagca caattttcaa agggttaggg tggataagag tacttattat
     1141 ataaagtttt catattacct acttcatatt acctattttt tgaaattaat aagttttatt
     1201 ttgcagattt tgcattttca tctgacttta tatactttat attatctgct gataactttt
     1261 gcatgtcaag tgcttttagt gtatcttcgt taattattat ttcagtttca tctaatgtag
     1321 taactggtat atcgccaaca gattttcctt ctaatacttc aatagcaagt tctccagcct
     1381 tataacctag tttttcataa tctattccac aacaagctaa agcaccagaa gatacagaac
     1441 cttcttcaga ggctattata ggaatcttgt tttcattagc aactttagaa actattggca
     1501 tagaagaaac tattaaattg tcagtaggga catataaaac atctattttg ccaactaaac
     1561 tagaaatagc ttggttaact tcacttgaag tgctgattcc tttttcaact acttcataat
     1621 tatttttctt agcataatca tgtagagaat caacctggat ttttgaatta acttcactag
     1681 tattgtatat aacccctatt ttctttgctt ttggagtcaa tgttttaacc aattctaatg
     1741 ttttatcaat tgaaagataa tcagatgtac cagaaacatt tccacctggt ttttcaagag
     1801 atttaacaag tccagcttct acagggtctg taacagcagt cattattatt ggaatatctt
     1861 tagtagcatt ataagctgct tgtgcagatg gagtagatat agcatatatc aaatcttttt
     1921 tgtcggatac aaacttacta gcaatacttt gtgtagtagg catatcattt tgtgcatttt
     1981 ggaaatctat ttttatatta tctccatctt tatagccttt atcttcgagt gctttgatga
     2041 atcctttctt tgctttatcc agagacggat gttccactaa ttgactaata cctatatttt
     2101 tttgtttttt gctatctgtt tttttatttt catttgaagc attggagcca tcattttgag
     2161 aacatccagt aagcattgat acaccaagta ctcctgctat aattaaactt aatatttttt
     2221 tactttttat cattttattt tcctccctta aaatttaata attatatttg ttcatatatt
     2281 catatgatag attataaata tttctgatta aaactaagat tttaatttct tatactgcat
     2341 attcaaattt ttctaagatt tcttcaactg ttaaattttc tttttcttta tcttttacat
     2401 ctaatacaac ttcaccctta tgaagcatta tcaacctatc tccatattga agagcgtgtt
     2461 tcaagttgtg agtaaccata agtgtagtta tatttttttc tcttacgatt ttatctgtta
     2521 actctattac ttcatttgat gtttttgggt caagtgctgc tgtatgttcg tctagtaata
     2581 agaccttagg acttgctaag ctagacataa ttagtgatag tgattgtctt tgacctcctg
     2641 aaagatattg tactttaata tctaaatatt tttttaagtc caaagaaatt ccttcaagta
     2701 agtgttccaa attattcgtt ttatgacgaa gacatttttt taagttcaat aatttccctt
     2761 tattaagtgc tagagataaa ttttctctaa ccgtcataga aggacaagtt ccaaggcttg
     2821 ggtcttgaaa aactctactt actatttgag ttcttttata ctcagctaaa tttgttatag
     2881 tagtactatc taaagtaatt tgaccagaag attcttttat aagccc
//
LOCUS       DAGQWR010000250         2858 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.255, whole genome shotgun sequence.
ACCESSION   DAGQWR010000250 DAGQWR010000000
VERSION     DAGQWR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.255"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            18..869
                     /locus_tag="MIW95_003813"
     CDS             18..869
                     /locus_tag="MIW95_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBY3153103.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1002..2615)
                     /gene="ggt"
                     /locus_tag="MIW95_003814"
     CDS             complement(1002..2615)
                     /gene="ggt"
                     /locus_tag="MIW95_003814"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBY3153104.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
BASE COUNT          906 a          481 c          395 g         1076 t
ORIGIN      
        1 cataggaggt acattctttg aaaaacatta tttcagcaaa agaacttata tcaaaactag
       61 aaaaaaatga taatctagtt gtaatagatt gcagatttga tcttatcaat agaacttatg
      121 gtattgattc ttataaaaaa ggacatatca aaggcagttt tatcttagat atagacaagg
      181 atttatcatc tcctacaaaa gaacatggtg gtaaaaatcc tttacaagac cctttaatac
      241 taaaagaaaa attagaaaaa atgggtgttg ataatgacac tacgattgta acttatgatg
      301 atggagattt aaatggagct tgcagattat ttttccaatt aaaacatttg gggcttaaaa
      361 atgtctatgt actagatggg ggaataacat ctttcgtaaa ggaaggtgga gaattggaag
      421 agaaagtgaa tataccaagt tgtacaggta aggaaattag accaaatata aacaatgatt
      481 tagttgttcc aatggaatat gttaagtcta aattatacaa aaaagatact gtaataatag
      541 attgccgtgc taatgagaga taccagggtt tagttgaacc tgcttactca aaagctggtc
      601 atataccaag tgcaaaaaat tatttttgta aggatttaat aaaaagtgac tttgaaaatg
      661 gctcacttaa agatatagaa tttttaaaaa aattctttaa agatttaaat aattatgatg
      721 aagtaatttt atcttgtggt tcagggatat ctgcttgtgt taacagtctt gctcttagag
      781 aactggatat acctcacaag atttatattg gtagctttag tgactggatt agttatgatg
      841 acaatgaaat caaaactggt caagaataag gctcataatc tatataaaat cagcatatga
      901 tagcttaaat tttgtaaagg cgtcagtcat tgtagacttt cgcctttact aattatttca
      961 tatactcttt aaatttatta tatatatttt agttgataat attaataaac tgcaatatga
     1021 ccatcacatc ttgattccgt cccacaaata taactttcct gttctgtttt aaatattatt
     1081 tgacctcgtc ccatgtcgac agtatcatga attactttta tttcatgacc cttttcaatt
     1141 aatgaatcta ttatatgctt tggcatctct ggttcaactt ctatttcctt tccttttatc
     1201 cactgccatc ttggagcatc tagcgcctct tgtggattta atccaaaatc aatcatattt
     1261 gttaacactt gaatatgtcc ttgtggttgc ataaaagctc ccattacacc aaatggacct
     1321 attgctttat cttctttacc taaaaatcca ggtattatag tatgataagg ttttttaaat
     1381 ggttttacta cattgtggtg tttagggtct aaattaaaat tatttcctct actatgtaaa
     1441 gcaatcccag ttcttggtat cacaattcct gaaccaaaat ttatgtaatt gctctgtata
     1501 tatgaaacca tatttccatc cttatctgca gtacaaaggt atactgtacc accacaatat
     1561 ggctctcctg cagttggata caaagcctct ttattgtcaa ttaataaact tctttttttg
     1621 gcatactctt ggcttagtaa ttcctgaatc tttaccttca tatgctctat atcagttaca
     1681 tatgttttag aatctgcaaa agctaatttt aaactctcta taattttatg tatgtcttct
     1741 acattttcta tatttccttc taattccaat tcttttaata tgttaagtgc cattaaaact
     1801 gttattccat gaccatttgg tgggatttca aatattttat atcccttata ttctgtactt
     1861 ataggttcta cccatgttgg ataaaaacac tctaagtcac tctttcttat agccccatta
     1921 aattttttag aatattcatc tattttatca gcaagtcttc ctctataaaa gctttcagcc
     1981 tgagtatttg ctatctcttc taatgtactt gcctgttctt cacatataaa tatatctcca
     2041 gcctctggag ctttaccatc tttagaaaat gtatcaaacc atggtttgaa ctcttctcca
     2101 ataagttctt tttcatagag ttcatatgat tttttccata ccttagctac attaggagat
     2161 actacgtatc cctctcttgc atagtttatc gctggagata agcattccat taagctaagt
     2221 ttaccatatt ttttatttaa ttctgaccaa gctgcgggta ttccaggtac agttactgct
     2281 ccaaacccat attttggcat ttctttcata ttattataat tttttaattc catattttct
     2341 ggagcaaagc cactagaatt taaaccatac aatcgttttt cttcctctat ccaaacaagg
     2401 gcatatgcat ctcctccaat cccattgctt gtaggttcta ctactgctaa tgtggcggct
     2461 gttgcaactg ctgcatcaac agcatttcca ccctttttta gtatttcaag tccagcttga
     2521 gatgctaatg gtgttgatgt tgcaactgct ccatttttag catacacgac atttcttatt
     2581 gatgagtatt tatgttggta ggcattaaac ttcataaatt tcagctcctc gtcatttttt
     2641 atttgatatt ctgcttagat ttattatata acatcaagta atgtaactga tacaaaatga
     2701 tataaaatat agactttttc ctaactttaa ttttatcttt aaataaatat cttaaactca
     2761 attctagtat tttctatctg cacttaaata agttatttta ttcaaaacat atatctaatt
     2821 cttttgactt aaaaataatt tattctttta tcatgttt
//
LOCUS       DAGQWR010000251         2856 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.256, whole genome shotgun sequence.
ACCESSION   DAGQWR010000251 DAGQWR010000000
VERSION     DAGQWR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.256"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            138..506
                     /locus_tag="MIW95_003815"
     CDS             138..506
                     /locus_tag="MIW95_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBY3153105.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            509..880
                     /locus_tag="MIW95_003816"
     CDS             509..880
                     /locus_tag="MIW95_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBY3153106.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1024..1479)
                     /locus_tag="MIW95_003817"
     CDS             complement(1024..1479)
                     /locus_tag="MIW95_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153107.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1518..2783)
                     /locus_tag="MIW95_003818"
     CDS             complement(1518..2783)
                     /locus_tag="MIW95_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBY3153108.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1052 a          402 c          398 g         1004 t
ORIGIN      
        1 taataaaatt caaagttata ttagtagaaa ttggaattat ttataatata ctccaaaatt
       61 taaaaataat ggttgaaaaa tctaattaag agtactataa tttaattaag taattactta
      121 atcaaaacga ggtacttatg gaaaacttgg ttaaaatatt taaagcattg agtgatgaaa
      181 ctagacttaa catacttata ttagtatcca agagaaatat atgtcaaaag gggatttcta
      241 aatatctagg gatttcagat tctgctgttt cacaacatat aaaaatatta aaagatgttg
      301 ggataataac aggatataaa gaaggatact atgttttata tcatattaat aaagaatcat
      361 ttaatatatg tgtagatttt ataaattcta tgttaagtaa ttctagtgaa agctttatag
      421 atgtatttga tgtaaataca atacatctag gatgcagtaa agaatgtaaa tctataaaaa
      481 aatgttgtaa gagaagggag aaataataat gaaagtatgt ataccagtag aggagaataa
      541 aggattggat agtaaaccat atggacattt tggttctgct ccaatttttg ttgtgtgtga
      601 tttagaaagt ggagaagtta aatctttaga taatggagat ttagatcatg aacatggaaa
      661 atgtcaacca ttaaaagcat tgtcagggac agctgtagat gcagtagttg ttggaggaat
      721 aggacaagga gctataatta aattaaatgg aatgggaata aaagtttaca gagcagaagg
      781 tgatactata agtgctaatt tagacttact taaaaatgga aaattagtag agtttccatc
      841 agaccatact tgctcacatg acggatgtgg acatcattaa aaaaaacagc tgatgaaata
      901 tatacttcat cagctatttt ttcttttaat aaatacgatt ttattctttt aaaaccaaaa
      961 tttagatttt tatgtttttt gtagtatcct taataatatt tcaagtttac aagtttacta
     1021 cttttagatt ttttttgtat gtttttctat atagtcaata tcatttaata tgtcattgag
     1081 ttctttatta agtgttttta aatcatcacc atctacattg tagtttaaaa atagttcctc
     1141 tacattatca atgatagaat tgacttttct agtaacttca acaaatgtct taaagttact
     1201 actattttta gcatttagaa atttttttaa atcctttgat ataaatgata ctatttcata
     1261 ttcatcatta aatagacctc taccaatagg atatggttca attctctgta aaaaatattg
     1321 gtctttagca ttaatattat aagtataaga tatttcccaa ccgtctccta aatcaaactt
     1381 gataaaatat aatgtactac ttacttttga tattcttgtg gcaccaagct gtcttaaaag
     1441 aacttcaatc gaatcacctt gaacagtatt aatattcata tatacacatc ccctttagta
     1501 ttattatact atatttttta aaattttata attaaataaa cagtagatat aataatagat
     1561 ataatcataa gtggaaaacc tattttgaaa tactccttaa aagagatagg atgaccgtgt
     1621 ttattaccta tacctgctag tacaacatta gcagatgctc ctattagtgt tccatttcct
     1681 ccaagacaag ctccaagaga tgttgcccac catagtggca ttacatcaat accttcagct
     1741 tgcatagtca aaataagagg aattagtgtt gcaacaaatg gaatattatc taagaaagaa
     1801 gagactatag ctgataacca taatatcaac aacattgtaa atacaagatg tccttctgta
     1861 agtccaatca atgcttgagc caatttgttt attatcccaa cttctactag tccaccaact
     1921 actacaaata atcccataaa gaataaaatt gtggaccatt caatacctgc cataatttca
     1981 tctacatctt gttttcctat taaaagcatt atagatgctc ctgtcagtgc tacaacagaa
     2041 gaatctatgt ggattgtact gtgaaacata aaaccaaaaa gtatgaatgc tattaatata
     2101 aggcttttta taagtagagg tttatcttta acagattttt tttcatctag ttttaatatt
     2161 gcatttttag ctctttcatt cacaacaagt tcttttccat aaataaatct aaagcatatt
     2221 atagttatag caagtattac tataacagca ggacctaaat ttattacaaa atccataaaa
     2281 ctaagattgg cagcactacc aatcataata tttggagggt ctcctataag agtagctgtt
     2341 cctcctatat ttgaagctaa aatctgtgtt attaaaaatg gaactggatt aagtcctaat
     2401 atttgtgtaa taactatagt cattggtcca ataagtaaga cggttgtaac attatctaat
     2461 acagctgata aaatagctgt tataatagca aaacaaatca ttatcttcca tgggtcacct
     2521 ttagctttct ttgcagtcca gatagctata tattcaaata accctgagtt tttgacaaca
     2581 gctacaaata gcatcatacc aactaataca ccaatagtat tgaaatctat atggcttaat
     2641 ccttcatcaa gtgtaagtat attgaatatt atcattaata gtgcaccacc aacagctgct
     2701 acggatctgt ttaccttttc tgtaataata gctgccataa ctattaaaaa tatacatata
     2761 gctattattt gttgaaattc cataatataa gtacctccat ttgtattatt ttgtgcctaa
     2821 tgctagtact atatatagta ttactttttt attgtt
//
LOCUS       DAGQWR010000252         2840 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.257, whole genome shotgun sequence.
ACCESSION   DAGQWR010000252 DAGQWR010000000
VERSION     DAGQWR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.257"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..1374
                     /locus_tag="MIW95_003819"
     CDS             <1..1374
                     /locus_tag="MIW95_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBY3153109.1"
                     /translation="KRFEQLEEAQNNKSVKYYLELEKQYKLAINSIEKDILAWYNRFA
                     KNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENASARVHITRL
                     EALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWSLMSLDTNR
                     INKIISKPWAIDGLNFSERIWGKYRPTLVNELHTKLTQSIIRGENPKNLVNDFAKRFN
                     VSKSQAKNLIMTESAFFASASRKDCFSDLEVEKYEIIATLDLRTSNICRELDGKVFDM
                     KDYQVGVTAPPFHCRCRTTTAPWFEDEEGYRAAKGEDGKTYYVPSSMKYNEWYEKYVK
                     HNSILEIKNSAIIDSIKEDIKNGKYNLNIHDGKQGKHLKEHNNYIEGRSYLTITKEEA
                     QELVNKHAGNGIIKFNRSGEWDKKELIEVDKNIGVNVNNITGEKTLTNKFKIHYSKTG
                     THIVPAL"
     gene            1389..1589
                     /locus_tag="MIW95_003820"
     CDS             1389..1589
                     /locus_tag="MIW95_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153110.1"
                     /translation="MKLWEYVGKNVQITCVDKQIIRGKCDGYTQALDNEPEIASISIA
                     RDGYGIEVYENEIESIENINKE"
     gene            1655..2218
                     /locus_tag="MIW95_003821"
     CDS             1655..2218
                     /locus_tag="MIW95_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBY3153111.1"
                     /translation="MDWLKELLEGIKIEDNKIDVASLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            2230..>2840
                     /locus_tag="MIW95_003822"
     CDS             2230..>2840
                     /locus_tag="MIW95_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBY3153112.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTT"
BASE COUNT         1247 a          302 c          527 g          764 t
ORIGIN      
        1 aagagatttg aacaattaga agaagctcaa aataacaaaa gtgtaaaata ttatcttgaa
       61 ttagaaaagc aatataaact agctataaat agtatagaaa aagatatatt agcatggtac
      121 aacagatttg ccaaaaatga aggaatatct ttattagaag ctaagaaact actaaataca
      181 agagaactag aagagtttaa atggagtgtt gaagaatata ttaaatatgg taaagaaaat
      241 gctataaatc aaaagtggat gaaagagtta gaaaatgcta gtgcaagagt tcatataaca
      301 aggcttgaag ctttaaagtt acaaatacag caacaagtag aagttttata tggaaatgaa
      361 cttgatggta ttgataaact aatgagagat atttatacaa gtggatacta tcatacagct
      421 tttaatgttc aacaaggagt aaacgttggt tggagtttaa tgagtcttga tactaataga
      481 ataaataaaa ttatctctaa accatgggca atagatggat taaactttag tgaaagaatt
      541 tggggtaagt atagacctac tttagtaaat gaactacata ctaagctaac tcaatcaatt
      601 attagaggtg aaaatccaaa aaatttagta aatgactttg ctaagagatt taatgtatct
      661 aaatcacaag ctaagaattt gataatgact gaatcagctt tctttgcatc agcaagtaga
      721 aaagattgtt ttagtgattt agaagtagag aaatatgaga ttattgctac attagattta
      781 agaacttcaa atatatgcag agagttagat ggaaaagtat ttgatatgaa ggattatcaa
      841 gttggagtta cagctccacc atttcattgt cgttgtagga caacaacagc tccttggttt
      901 gaggatgaag aaggttacag agcagcaaaa ggagaagatg gaaaaacata ttatgtacca
      961 tctagtatga agtataatga gtggtatgag aagtatgtta aacataatag tatcttagaa
     1021 ataaaaaata gtgctataat agatagcata aaagaagata ttaaaaatgg taaatataat
     1081 ttaaatattc atgatgggaa acaaggaaaa catttaaaag agcataataa ttatatagaa
     1141 ggaagaagct atttaactat aacaaaagaa gaagctcaag aacttgtgaa taagcatgct
     1201 ggcaatggaa tcataaaatt taatcgaagt ggagaatggg ataaaaaaga acttatagaa
     1261 gttgataaga atataggggt caatgttaat aatattacgg gtgaaaaaac tcttacaaat
     1321 aagtttaaga tacattattc caaaactgga acacatatag taccagcttt ataaggagga
     1381 aaaataaaat gaaattatgg gaatatgtag ggaaaaatgt tcaaataact tgtgtagata
     1441 agcaaataat aagaggaaag tgtgatggat atacacaagc tttagataat gaaccagaaa
     1501 tagcaagtat atcaatagct agggatggtt atggaattga agtttatgaa aatgaaatag
     1561 aatctatcga aaatataaat aaggaatgac taagcatgta cttaaaaata agtagatgct
     1621 tttattttgt aaaaaatgaa aggagaaatt taaaatggat tggttaaaag aattgctaga
     1681 aggaataaaa atagaggata acaagattga tgtagcttct ttacaaaagt ctatagaaaa
     1741 gaaaataaaa gagactacaa ttactcaaga agattataca aatcttgaaa cacagcttaa
     1801 tacagctaat gaaactatta aaaagtttga aggaggtatg acaaaagaag atgtagagaa
     1861 tctaaaaaca acttatgaaa ctgataagaa aactttggaa gaaacctaca aaaaagaaat
     1921 tgaagaaaag gactttaatt actggttaaa tgatgctttt aagtctatta aatgtaggga
     1981 tgaaatagcg ttaaaagctc atttagatat agaagcacta agaaatagta aagatagaca
     2041 aaaagctttt gaagagcaaa taaacccttt gaaacaggat aaagattatt tgtttaatgc
     2101 aacactagaa ggtgaagagc ctaaaataga tactataaca ccagggcaag agcctaagat
     2161 aaatgatttt ggttttaatt ttactggggt aagacctcat gaaaataata ataaatagga
     2221 ggaaataaaa tggcagcact aaattatgca aaagaatatt caaatgtttt agcacaagca
     2281 tatccttata ctttaaactt cggggatttg tatgcaacac caaataatgg aagatataga
     2341 tggactggtt ctaaaacaat agaaatacca actatatcta caactggaag agtagattca
     2401 aacagagata caatagcagt agctcaaaga aactatgata atgcttggga acctaaggta
     2461 ttaactaatc aaaggaaatg gtcaacattg gttcatccag cagatataaa ccaaactaat
     2521 tatgtggctt caataggcaa tataacaaaa gtatataatg aggaacaaaa gtttccagag
     2581 atggatgctt actgtatatc taaaatatat gctgattgga ccgcattagg taatacagca
     2641 gatacaactg ttcttacaac aacaaacgta ttagaagtat ttgataagtt aatggaaaaa
     2701 atgacagaag ctagagtacc tgaaaatgga agaatattgt atgttactcc agtagtaaat
     2761 acacttatca aaaatgcaaa agagatacaa agaacagtaa atataaagga tgcaggaact
     2821 tctcttaatc gtcaaacaac
//
LOCUS       DAGQWR010000253         2785 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.258, whole genome shotgun sequence.
ACCESSION   DAGQWR010000253 DAGQWR010000000
VERSION     DAGQWR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.258"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(13..552)
                     /locus_tag="MIW95_003823"
     CDS             complement(13..552)
                     /locus_tag="MIW95_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBY3153113.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPILVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(653..1333)
                     /locus_tag="MIW95_003824"
     CDS             complement(653..1333)
                     /locus_tag="MIW95_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBY3153114.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(1362..2651)
                     /locus_tag="MIW95_003825"
     CDS             complement(1362..2651)
                     /locus_tag="MIW95_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBY3153115.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT          942 a          500 c          259 g         1084 t
ORIGIN      
        1 atatttttaa agttatcttt ctactatatc tttatttccc ctcaaaaatt ttatagtaag
       61 aatttcatcc acaaacaatg ttactaaaag tatagttaca agaattggcc aataaggtat
      121 tataagacta tataaactat caactatata cacttgaaaa tatctaactc caataaatgt
      181 caatatagtc caatatacag aaaactttag acatactatt cctctactat tgtacttata
      241 atctgaataa tcccaataga ccttattaaa atatactttc ataagtattc ctgtaatata
      301 ttctactact gtcggcacta ctagacataa taaaagcaat gcaaatatat tattggggtc
      361 aataaatctt gagacctcta ttataatcga cattgcaatt gcatacattg gtttaaatgg
      421 accatttaaa aatccatctt cttgaaaatg cccttgttta taataactat ataagttttc
      481 aattatccaa cctataaaac catacaagaa aaaattaaat aaaaaatata atagagaacc
      541 tacattatcc atgaatattc ctcctagctg tttatactta ttttttctca tattcatgga
      601 tttattctaa atcaaagaaa ttatatttaa tttatttaat taatacttta tattatagat
      661 tgtgacattc tttagagttc aaacttttta ggtattctct aacttccagt aaattttcaa
      721 gtatcttatg tgattgtgat ttaatttgct catctggctc ttttaaatct ggtacattta
      781 tacatcttat cccaccattg tatgcagctt caactcccat aggcgaatcc tctatgacaa
      841 tacaattttg gggattaaca tttatctttt tagctgcttt taagaatatc tcagggtttg
      901 gtttagagtt tacaacatca tctccacaaa ctatagcatc aaagtaatct tttaaatttg
      961 ccttagctaa tctctttact gctctttctc tctttgttga tgttgcaaca gccattttgt
     1021 atccattttc ctttaaaaaa gagattagtt cattgactcc taatttgata ggagctccct
     1081 tcctctccat aaattcaatc atatttttag ttttttcgtc atataaatct attattggta
     1141 cagaactatc gtatatatct gtaagacctt ctattattcc tttacgattt cttcccataa
     1201 ctgatgtata aatttcctta gtcatagtat atccatactt ttcaaacgtc tccatccaaa
     1261 attcaagaga aattctctct gagtcaaaca aaactccatc catgtcaaaa ataattcctt
     1321 ctactttttg catacaattc ctccaatatt ttttgatata attatattac actttttatg
     1381 attaatttta caaccctcaa tttatccaat tctaatattg taaaattgta attttcaaaa
     1441 gttacgcttt ggtttacttt aatatttgaa ccaagcatat aggatatcca cccacctata
     1501 ctgtcaaatc catcatgctc tatatcaagt ccaaaatact tattaatata gttgatagat
     1561 tcagttccat caactaagta agaacctgct cctattttct taatatgagt ctcttctttg
     1621 tcaaattcat cttgaatttc tccaactatc tcttctagta tatcttctat tgttaccaca
     1681 ccagatgtac caccatattc atccacaact atagcaagtt gaagtttttc ctttcttatt
     1741 ctctcaagag ctttatctat agttaaattt tcagatatat atataatatc tctcaatatt
     1801 tcttctagtt caattttatt ttcatttatc ttttgattgt ataaatctct tatatgtaca
     1861 aaacctaata tatcatcttt attttttctg catactggat atcttgtaac accttcctct
     1921 tttaaaatag ctaatatctt ttcttcagaa tcactttcat atatacatac catatcagtt
     1981 ctaggaacca ttatctctct aattttcttt tcttcaaatt caaaaatatt atcaactaat
     2041 ctttgctctg attcatctat aagtccactt ttataacttt cttctactaa tagtcttatt
     2101 tcgtcacctg tatggggttc atcaacctca tcagcttgtg aatatccaaa tggtttaaga
     2161 aataaatcag tacttaagtt gaaaaaatat attattggat atgttaactt ataaaatcct
     2221 actaataaaa aagatgtact caacattatt ttttctgtat tataaagtgc taaagcttta
     2281 ggaaccaact ctcctattac aacctccatc attgttataa ttaaaaaaga aatggaaatt
     2341 gatatagtat atataatact ttcactaaga ttaaatacac ttactattgg taaaattaac
     2401 tctttaactg tagattctcc catccaacct aatgcaagtg aacaaagtgt tataccaagt
     2461 tgacatgcag aaagatagga atttaagttg tcttttatta ttttacatct ttttgcatta
     2521 ttattacctt cagttatcat ggtatcaatc ctagaactac gaagttttac catagcaaat
     2581 tcacaagcta caaaaaaagc atttatcaaa acaagcatta ctataagtgt tacttttaat
     2641 aactcccgca taaagtattg gttcttataa tgtaagaaat ctataagaac tcaaagttcc
     2701 ccccttatat ttataaattt aatttataat atatattcta tcatatttat taatatatcc
     2761 tactttatta caaataattt ttata
//
LOCUS       DAGQWR010000254         2778 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.259, whole genome shotgun sequence.
ACCESSION   DAGQWR010000254 DAGQWR010000000
VERSION     DAGQWR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.259"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            295..789
                     /locus_tag="MIW95_003826"
     CDS             295..789
                     /locus_tag="MIW95_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBY3153116.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIIALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKNDYGNLIDITNDQNKINIVIKLLNN
                     KSNT"
     gene            1186..1336
                     /locus_tag="MIW95_003827"
                     /pseudo
     CDS             1186..1336
                     /locus_tag="MIW95_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1812..2000)
                     /locus_tag="MIW95_003828"
     CDS             complement(1812..2000)
                     /locus_tag="MIW95_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY3153117.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGEGALKIKKFSILYGSS"
     gene            2162..2512
                     /locus_tag="MIW95_003829"
     CDS             2162..2512
                     /locus_tag="MIW95_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBY3153118.1"
                     /translation="MIQKKIFMGFCGFLGFLSLRYFSSGNVTDLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNVELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            2514..2714
                     /locus_tag="MIW95_003830"
     CDS             2514..2714
                     /locus_tag="MIW95_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3153119.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
BASE COUNT         1023 a          327 c          350 g         1078 t
ORIGIN      
        1 attggtaagt aagtataatt aaatagacat tattttctgg gtcctctttt tattttcatc
       61 tgcttttaaa taatattatt gacattgata tgactgtagt tttctttatt ttaatccatt
      121 gcttatatct tttctaacaa gaaatgtcat tttaaatcat ttctataaat tgtaaaataa
      181 tagtatattt gtcatagaaa gtgatttttt tgtctatata tggtaaaata tagaaagtag
      241 aaatcaacaa aataaaaatt tatatattat taattttata tggaggggaa ataaatgaaa
      301 aataaaaaag gatttactct agtggaatta ttagtagtaa ttgctataat aggaatattg
      361 gcaataatag cacttccagc attatttaaa aatatagaaa aagcaaagat agctaaactt
      421 gaagctgata taagtgcaat aaaaagtgca tctcttagtt actatgctga tgaatccaag
      481 tatactgatg gaggaatgat atcatgggta aagaaagatg gaaaaataat aataaatggg
      541 ggttttaaag atgacccatt agcagataaa atagaaaatt tagggatgcc ttataatggt
      601 tcatatctgt taatgtcatc tcctggtcat gaaaaatatc tagaattaag catacttcca
      661 gaaggagaaa taagcaaaag tggtctagat aaattaaaaa atgattatgg aaatttaata
      721 gacataacga acgatcaaaa taaaataaat attgtaataa aacttttaaa taataaatcg
      781 aatacttaaa tacaataaat ttgatttaat aattactgat gaaataaaca atcttgtttg
      841 gagaaatgaa aaaagcactc cccaagaata gaatgcttag ttataactac agaagtgtat
      901 tggcatacac ttctattttt tattatatca cataatttat gttgctaaaa atacctattt
      961 aagttgtttt taaattgtgt tgagtaatat tcttgatagt ttatacatta atgaatttca
     1021 aaaaaataag cactctttat aataaaaagt actttagtgt atgttaatgt cctatctaat
     1081 aaaattgtaa catgtcttat gctttagtat gataattttc attctcttta ttgttgcaac
     1141 ttatactata gttttgctaa tttatctgca aacacaactt ctttattgca tgtgtatcaa
     1201 ccaaaaacat cagaatactt acttaataca tctgcaaaat cgtatctttc atctatagta
     1261 ttcttatttg acaatataac aaaaaatgaa tcgagatatg ccttttttat aattagatat
     1321 attttataaa ttctagcata tcatttgtta ttttctgata tgctattgat gttttaaatc
     1381 ttctatttat tttattattt tttacttttc caattgtacc tttataggag ttatatttat
     1441 gtgatttatg aaaaaatgct atgcatttaa gttctaattt tttgataata catctaactt
     1501 tcttttgatt aataatgata ccactatttt taatgtggct tccctgaggc acacatcttt
     1561 tttaaaaact caagctcatc ttttaaaaat ttattttcat attctaattc ttgttctctt
     1621 gttaatttat tttttcttta gtgtttttgt ttttattcat tatggtcttc ctttcatttt
     1681 gaaaaaggca tcaacaccac tatgtaaaaa tttttgtttt catgttgcta taactgtgct
     1741 attgtttatt ccaaaatact tagcgattta ttgtacacta tacttagtta tcatcatata
     1801 gtttataaca tctaacttga accatatagt atagaaaact tttttatttt tagtgcaccc
     1861 tctcctaata tattaaccca tctttcaact tgagaatggt cataaactcc atattgtttt
     1921 actattaaaa tatctcttcc ttctctattc aaataaaatt tagctacttt taatttgaaa
     1981 ttaaaatcat atttagtcat acataaacat ctcaagcgtt agatttttac tctaacaatt
     2041 atggtgcagt acaaatattg tataatactg attttctatt gacaaaagaa tataaaagag
     2101 atatatttgt attataaaga tacaaatata tctcttttaa aattactaag gaggataaaa
     2161 aatgattcaa aaaaaaattt ttatgggttt ttgtggtttc cttggatttt taagtcttag
     2221 atatttttca tctggcaatg ttactgatct tacatatatt ggattttttg cttttttctc
     2281 aaactttatt atagcaaaaa taaatggtga taaagcagat gaaaggtatg tacaagatga
     2341 aaaagcagct atggcattta caggacagct tgctattata gaattattca ttttatggtg
     2401 tattactata gtgtctagga atgtagaatt aatgtgtgtt ttactatcaa ttacatatgc
     2461 aatcacttta aatgtttatg ctataaaact ttatatttta gaggaaaaat aatatgccca
     2521 aattcatttg taacctaaaa aaatatagac aattaaaaga attaactcaa gaacaattag
     2581 cagaaaaagt aggagttaga agagaaacca ttatgcgttt agaagcagga aagtataatc
     2641 cttctttgaa attagcaata gatatatcaa aaatagtaga tacacctatt gatgaattgt
     2701 ttatttttga ttagtttttg cattattaaa aatgtgtttt aaatacaaac ttaaattcaa
     2761 tctttaagtc actcctga
//
LOCUS       DAGQWR010000255         2777 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.260, whole genome shotgun sequence.
ACCESSION   DAGQWR010000255 DAGQWR010000000
VERSION     DAGQWR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.260"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            458..2269
                     /locus_tag="MIW95_003831"
     CDS             458..2269
                     /locus_tag="MIW95_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBY3153120.1"
                     /translation="MKKKYDFLIYSAIWLVIIIFFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILYNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2357..>2777)
                     /locus_tag="MIW95_003832"
     CDS             complement(2357..>2777)
                     /locus_tag="MIW95_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153121.1"
                     /translation="PEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHYWDEKVATE
                     KTFINGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYV
                     ELTNDSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1062 a          281 c          408 g         1026 t
ORIGIN      
        1 ttgcacaaaa atagatataa tttaagtaat ataccaaaaa aaaggaatat atttacataa
       61 tatgataaaa taaattcaaa gagttaaatt gtaggtaaat agtattttaa ttatttatta
      121 aaatatagat tatgtatatg ccaaatttaa aaagagacat aaagacatta tacaaatata
      181 ttattggggt gatattttat aagaaaagaa gtgatgcagt aaattaattg agcaatttaa
      241 aaatgattta atgatagtgt tgatgaatgt ttaaaaagaa attttagaaa aacgtatatt
      301 gctgtttgtg ttggaggtga tgaatttgta taatttatag ggtataaatg attaaactac
      361 ttaagataaa tacatatcac tgtaaaatat tgataatcat tttttataag agattgatta
      421 tttgcaaatt atatatatta caaatgaggt aacaataatg aagaaaaaat atgatttttt
      481 aatttattct gctatttggc tagttattat tatttttttt atattttctc tatatcatag
      541 tatagagcat attaaaatta taagtcacac tggtactatt cgcagtgaaa ctcaaaaggt
      601 tgttaagcaa gaattgaata atgaaagaaa tgatgacttg ataaaacgac tggataatat
      661 tctgataaag ctaagaacag gtaatggaga aaatggattt caaagatgtg ataataaaga
      721 atttcaacaa aaactaaatc aaatggattc tatgtgggaa tctatgaaga aagaaattat
      781 aaaagttaga aatggtgctt caggtgataa attatataaa ttgagtgaag agtattctgt
      841 gttgtctaat caaattgttt ttatatctga aaaacactct aatgcaaaat tgtattcttt
      901 tgctactgca ctatttattt atcttatatt ctcaacaata agtcttttaa tttgggaata
      961 ttataataaa aaaaggttta aaagaatatt ttatactgat aatttaacaa aaataaaaaa
     1021 tcaggttgca tttgaaaaca gagcgattga aattttgtac aatgcatcta acaaagagta
     1081 tgtactgcta aatattgata ttgacaattt taaatatatt aatgatacac atggatatga
     1141 atatggagat aaagttctaa taatagttgc agctgcactt tctaaaactt ttaatatcaa
     1201 agaaacttgt gcaagaattg gttcagataa ttttgtaata cttgcaaaat atagagattc
     1261 tcttttagag gatattcggg aaatgctaac taatgctata atctctgaat tagatatgaa
     1321 tgtaactcaa actatatctt attgtatagg agcgtactta gttgaaattg ataatttagg
     1381 ttataaatcc atcaattcta taatggataa ggcaaatatt gcacacaagg taagtaaaac
     1441 aagaggaatt tcctccacag tatggtataa tgaaaatctg ttaaaacagt tacaaatgga
     1501 aaatagtatt tataattata tgtataaagc gatagaaaat gaagaatttc atatgtattt
     1561 gcaacctaaa tttcaaatat cttctttaaa tgttgtaagc gcagaagctc ttgtaagatg
     1621 gttttctcca gaacttggtt ttttatcacc agatgaattt attccattgt ttgaaaaaag
     1681 tggatttatt attgaattgg acttttatat gttaaaaaaa gcgtgttcat ttgtaaaaaa
     1741 aacgtttatg aaaaaaaatc aatatacata tccgattgca gttaattttt caagggttac
     1801 tatttaccaa aatagctttt atcaaaggtt tttagatact gttaaagaat atgaaattcc
     1861 ttttaagtat atagagatag aggtaactga aagtgctttt aatgaaatat ctcaaccagt
     1921 tatttcgatt ttagaggagc ttaaaaagtt aggattttta atttcgatgg atgattttgg
     1981 atcaggatac tcttcattaa gtcttttatg ttctctatct attaatggat tgaagttaga
     2041 taaaagctta cttaaagaaa catttaaccg ggagaaagta tatagtatta ttcagtgtat
     2101 tattgaaatg tcacatcgta taggcatgtc tgttgtatgt gaaggtattg aaactaaaaa
     2161 agacttggaa tttttaaata cagttaaatg tgatgtagga caagggtttt atttctctaa
     2221 accgatagaa gaaaaagaat tttttaacaa atatgtaact aaaaaataat ataattattt
     2281 ttatcagtct atacattgag tcattttata tttcgttaaa tgactcaatg tattgttatg
     2341 tattttaaaa tataaattat ttaaaacgtt ttttgtactt atatagaaat tcttctatat
     2401 tttttgattg ttctcttatt tcaactgctt ctatatctag taaataaagt atagaatcat
     2461 ttgtaagttc aacatattca ccatcttctg aaccgagtgc atgtctctca atttttaatc
     2521 ctttttcttc ataatatttt tttgtttcta tatataatga ttctcgcttt tgtatgtagt
     2581 ctttcacttc attatttgga aagttatatg aatccactaa tgtttcgcca tttataaaag
     2641 ttttctcggt agcaactttt tcatcccaat aatggatttc ttcccaagta aaaaatatct
     2701 cagttccatc tggaatgaat gaaaacctgg taataatata taaataatta ttgaaattta
     2761 cttcataata ttcagga
//
LOCUS       DAGQWR010000256         2727 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.261, whole genome shotgun sequence.
ACCESSION   DAGQWR010000256 DAGQWR010000000
VERSION     DAGQWR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.261"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            297..2432
                     /gene="rnr"
                     /locus_tag="MIW95_003833"
     CDS             297..2432
                     /gene="rnr"
                     /locus_tag="MIW95_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBY3153122.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILAKILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1192 a          262 c          494 g          779 t
ORIGIN      
        1 ccaatcctat ttgatggtgt ataattatga gataaagagt aatttaagca attatgataa
       61 agaataactt aagtaattat aatgatattt acaaataatt tataaaacat cataatttgt
      121 ttgaagttag tatgacaaag cttacattaa atcaaaaata taaataagta attttaactt
      181 gtaataataa taagaaaata tagaaaatta tgtgataagt aaaaaatgtt ttatctgaag
      241 aaatataaaa gacaatataa ataataatat aatgaaaagt aaaacaggag ggaaaaatga
      301 taccaggact taaagaaagt ttgctaggat taataaatga aagtgcatat aatcctctaa
      361 aaaaagaaga attagcagaa atatttgata tacattcatc agaaatgcca atgttttata
      421 attttttaaa tgaattagaa gaagatggtt atatctgctt tactaaaaaa ggaaaaatag
      481 tatctcccaa tcaaatggga tactttgttg gaaaatttgt atcacacaaa aagggttttg
      541 gatttgtaga atctgatgtt gaatatacac aggatttatt tatctcaagt gacaatataa
      601 atggtgctat gcataacgat agagttatgg cagaaatagt agtacctgca actgaagaaa
      661 agagagcaga aggaagaata gtaaaagtaa taaagagaga aataactaga atagtaggta
      721 ctttccaatc aagtaaaact tttggatttg taacaccaga caacaagaaa tttactaaag
      781 atatatatat acctaaaaaa cattttagtg gagcagtaga taatgataag gtagtatgtg
      841 aaattacagt atggccacaa gaggatagaa aaccagaagg caaaattata gagatactag
      901 gtcaaaaagg tgaaagaggc gtagaaatag attctataat aagagaacat ggtcttccag
      961 aagaatttcc aaagaaagta ttgcaagaag cagaagctgt agctgttgag attcctcaag
     1021 aagagataaa aagaagaaga gatttaagag atttaaacat atttactata gatggagatg
     1081 acgcaaaaga cttagatgat gccatatcaa tagaagtatt acctaatgga aacttcaaac
     1141 taggagttca catagctgat gttacacatt atgtaagaga gaaaaataaa cttgataagg
     1201 aagccttaaa aagagctaca tcagtttatt tagtagataa agtaattcca atgttgccaa
     1261 aaactctttc aaatggagta tgtagcttaa atccatttga agataagtta actctttcta
     1321 tatttatgga aatagaccat aaaggaaatg tggttaaaca tgaaatatgt gaatctgtaa
     1381 taaattcaaa agctagaatg acttatacag aagtatctga tatattagaa aaggatgatg
     1441 aaaaacttaa aaagactttt gaacatgttg ttgaagattt taaaaatgct gaaattttag
     1501 ctaagattct tatgtcaaga agagaaaaaa gaggagctat agacttcaat ttcccagagg
     1561 caaagataat tctaaatgga aaaggcgaag ttgtagatat aaagccatat gaaagaagaa
     1621 tatctaataa gataatagaa gaatttatgc ttataagtaa tgagacaatt gccgaacatt
     1681 tttactggat gggaataccg tttgtgtata gaatacatga gacaccatct ttggaaaaaa
     1741 tggaagaatt gagtaagttt atatctacat ttggatatat aataaaaggt gataaagagg
     1801 aagtacatcc aaaagcatta caaggtataa tagagaaaat aaagggcaaa aaagaagaag
     1861 gagctatcag tactataatg cttcgttctt taaaacaagc taagtactca ccagaatgta
     1921 gtggacattt tggattagca gctaaattct actgtcactt tacttcacca ataagaagat
     1981 atcctgacct tcaaattcat agaataataa aagaatcttt aaataataaa ataagtggaa
     2041 aaagacaaga acagcttact actatagtgg attatgcatc tgttcaatca tccgagaaag
     2101 aaagaaaggc agagttagct gaaagagatg tacatgattt ctataaggct ctttatatga
     2161 aggataaagt tggtcaagaa tttgagggtg ttgtatctag cgtaacttca tttggaatat
     2221 ttgttgagtt agataatact atagaaggat tgataagact tgcaaatatg aatgatgatt
     2281 actatattta tgatgagagt tcttatagtg ttttaggaga aagaactaag aagtcatata
     2341 agataggaga ccttgttaag ataaaggttg aaagtgttaa tgtagatttt aaagaaattg
     2401 actttgagat agttgaaaaa atagaggagt agataaaaca gctaaaataa gagataacaa
     2461 gttgtagtat ttatgatatg tataaatata tagtgcacaa tactctataa aataaatgat
     2521 aaaaataggt aattaagaaa tatctaatgg aaatagttaa cttaaagata aataattaag
     2581 aaatatttaa tgaaagtagc taattgaaaa ataagtaatt gagaaatatt taatagaaag
     2641 agctaactga aagataagta attaagaaac atttaataga aaaagccaac tgaaaaataa
     2701 ataattaaga aatgtttaat ggaagtg
//
LOCUS       DAGQWR010000257         2719 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.262, whole genome shotgun sequence.
ACCESSION   DAGQWR010000257 DAGQWR010000000
VERSION     DAGQWR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.262"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(314..1210)
                     /locus_tag="MIW95_003834"
     CDS             complement(314..1210)
                     /locus_tag="MIW95_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY3153123.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDFLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1352..>2719
                     /locus_tag="MIW95_003835"
     CDS             1352..>2719
                     /locus_tag="MIW95_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY3153124.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVC"
BASE COUNT          929 a          386 c          317 g         1087 t
ORIGIN      
        1 aatcatacta ttttagctgt ttacttgctt atttgatagt tttatttctt tgattaaata
       61 tctttcagaa tctaagccag atgtctcaaa aaaactgatt tataatataa tcattttttc
      121 tgctattctt atttttccaa ttttagtttc atattaaaat atacttttca tagacactac
      181 attctttcat actcattttc acatttaact atacaagtag tcttactcat ttatgtacta
      241 tactttatta atatttatta agaaaaactc aaagatttaa aaatatatat tttctataaa
      301 gtatatttat gaattatttc atatcttctt ttaaaataac attactaact tctctatgtt
      361 catttctata cttagttgga gatatattat aattcctttt aaataagcga ctaaaataat
      421 ttatgctatc aaagccacat ctcatagcaa tttctgtcat atttaaatct gtttccttta
      481 aataatatat agctttatca agcctgattc catttataaa atcgattgta gtcttacctg
      541 ttatttgctt aaatgttcta caaaagtgat gtgcactcaa gttcacacga ctagcaagtt
      601 cttttaatgt aattttatcc atatattttt cttctatcaa gtcaaagata cattcaaatt
      661 tttctaaaga atactttctt tttataaatt catcttcaga atatatttta tcaatatgac
      721 ttcttaataa gagtactatt aattgataga tattagcttt tatagataac tcatatccaa
      781 cttctttatt gtgatattca tcaagtatag ttgtaataca ttttaaaatc agaaaatcat
      841 tttgaattaa attattaaat acaatatgat ttaaagccaa tggtgctaag tacttagttt
      901 gtaacaaatc aacttgatta ctaaaaagga atgtgggctc aatacgaata ctatagaatt
      961 ttaaattatc tgataagctt tctaaataat gtaattcatt actgtttgct attacaatat
     1021 ttccaggcac taccttaaaa ttatccaatc cacattcaag atatgctata ccatcaataa
     1081 aataatatat ttgtagatgc tcatgccaat gtcttttaaa gtttgctcct tttttaatgt
     1141 catcctttaa aaaaatatct ataaagtttt cttgaccttt cctattaaaa tatgtatttt
     1201 tatccttcat tattcccccc tcatcaaaat agtgctaatt ataagcaata tagttattgt
     1261 attcattatt tcatatcgct attatataaa atgtaactat cattgtaaac attaacataa
     1321 tgccagtcat tataataagg aggtaataaa aatgacaaac tcttttaaaa aagatgataa
     1381 tagaaaaaaa atcacagttt tatttgtagt cattgccatg acttttatgg caacattaga
     1441 ttcgagtatt ataaatgttg ctctcccagt tcttgcaagc aaactaaatg tatctctagc
     1501 atctattgaa tgggtaatag caagttactc aattattatt tgttcaacac tattattttt
     1561 tggaagactt ggagatatta ttggtaaatc aagagtattc caagtaggta ctattctctt
     1621 tacatcagct tcattactat gtggattaag taattcttta actttactta tagtttgccg
     1681 ctttattcaa ggaataggcg cttctgccta catggcaaac aatcatggaa ttatcacaga
     1741 actatttcca aaagaaagca gaggaaaagc cctagggatt ctagttactg cagttgctat
     1801 tggaaatatg gttggaccat ctgtgggagg atttattttg tcaatatttg attggaatgt
     1861 tatatttttt ataaatattc caatcggtct tattgtaatc tttttaaata caaaatttct
     1921 tcctaatagt aaaaaatctt cagaaaatat ggataaaact ggtgctatac ttcaattttt
     1981 aggaacaaca ttatttttta gtgctctaat tagtgctcaa caaacaggat tactaaatcc
     2041 atatatatta attgcactat tattatcaat tatttttatt atattatttt tgatattaga
     2101 aaaaaaacat cctcaaccgc ttcttgatct tgaaattttt agaaatttta aattttcatt
     2161 gaatttaatt tgtgctctta catcattcat ttgtattgca tcatcaagta ttttaattcc
     2221 attttactta caaagtacta tgaaacttcc ccctattcaa gcaggtttat ttatgatact
     2281 atcacctttg attttagcca ttttttcacc tatatttggg aatatatcag ataaaatcaa
     2341 gtcagaaaaa attattttaa ttggtcttct cgttatgagt tttggattct ttctaatgtc
     2401 aagattgaaa gaaagttctg cccttatttt atttgtaatt tatatatcga taatatcaat
     2461 aggacaagct atatttcaac ctgctaacaa tgctttgata atgtcaagct gttctagaag
     2521 taagctagga gttgtaggaa gcataaattc ccttgtaaga aatttaggac aagttatagg
     2581 tattactatt tctacaacac ttttatataa ttttatgagt attaaagctg gatatagggt
     2641 caatgattat gtgattaata atgataaaat atttgtattt ggaatgagaa atgtttatat
     2701 tattgttaca cttgtttgc
//
LOCUS       DAGQWR010000258         2647 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.263, whole genome shotgun sequence.
ACCESSION   DAGQWR010000258 DAGQWR010000000
VERSION     DAGQWR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.263"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..1208
                     /locus_tag="MIW95_003836"
     CDS             <1..1208
                     /locus_tag="MIW95_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBY3153125.1"
                     /translation="EANFTKKRYRAMKGSAGSGKSVNVAQDYILKLGDKKYQGANLLV
                     VRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIKSKVTGNSIIFRGVNDA
                     KQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGILTNPNLYYQMTFTFNP
                     VSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQMRKEQDPEGYKVYGLGE
                     WGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANAILRIGFKDGELYICNEIY
                     VHEMDTSEIIKIANSRGLEKTLFMYCDSAEPDRIKMWKSAGYKAKGVKKGPGSVKAQI
                     DYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEFMDDAMAALRYSIDNKL
                     KNNGISFLK"
     gene            1214..>2647
                     /locus_tag="MIW95_003837"
     CDS             1214..>2647
                     /locus_tag="MIW95_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBY3153126.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEMQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKSVQITFNHSMIINESEKIDMASKSIGIISDETIVSNHPWVEDVNDE
                     LERLKKQEETQKEYDDLIPTNNQEGVID"
BASE COUNT         1083 a          294 c          494 g          776 t
ORIGIN      
        1 aggaagctaa ttttactaaa aaaagataca gagcaatgaa aggttcagca gggagtggaa
       61 aatctgttaa tgtagcacaa gactatatac taaagttagg agataagaag tatcaaggag
      121 ctaatctatt agtagttaga aagtcagaag ctacacataa gtattcaacg tatgcagagc
      181 ttacaggagc tataaatcgt atttatggta aacaagctga taagtattgg aaaactactt
      241 taaatccttt agaaattaag agtaaagtta ctggtaactc tataattttc agaggagtta
      301 atgatgcaaa acaaagagaa aaattaaaat caattaactt ctcgaaagga aaattaacat
      361 gggtttggtg tgaagaagct acagaactta tggaaagtga catagatata ctagatgacc
      421 gtttaagagg tattttaact aatcctaact tgtattatca aatgacattt acatttaatc
      481 cagtctcagc tactcattgg ataaaaagaa agtattttga ctataaaaat gatgatatat
      541 ttactcatca tagtacttat ctacaaaata gattcataga tgaagcttac tacagaagaa
      601 tgcaaatgag aaaagagcaa gacccagaag ggtacaaagt atatggtctt ggagaatggg
      661 gagaaactgg tggagcaata cttaaaaatt atgttataca tgaatttccc acagaatttg
      721 aatactttga caatatgaga ctgtcacaag actttggctt taatcatgca aatgcaatac
      781 ttagaattgg ctttaaggat ggagagttat atatatgtaa tgaaatatat gtacatgaaa
      841 tggatacctc agaaatcata aaaattgcaa atagtagagg tttagaaaag actctattta
      901 tgtactgtga tagtgctgaa ccagatagaa ttaagatgtg gaagagtgca ggatataaag
      961 ctaaaggagt taaaaaagga ccaggaagtg ttaaagctca aatagattat ttgaaacaat
     1021 taagaataca tgtacatcct agttgcacta ataccataaa agaaatacaa caatggaaat
     1081 ggaaacaaga cgaaagaact ggattatatc ttgatgaacc agttgagttt atggatgatg
     1141 caatggctgc tcttagatat tctatagata ataagcttaa aaataatgga ataagcttct
     1201 taaagtaaag gaggtgttaa atatttatat aagtgaaaca gatttaataa aagttcagtt
     1261 aaaaaaagag agcaccttta acctagtaaa agtcatagaa cactacatct taaagcatag
     1321 gccagaaaaa tataaacaag gagaagaata ctattatggt aatactgatg taaacaataa
     1381 gagaagatat tatctcttag atggagctaa ggttgatgat tttactaaag ttaataataa
     1441 agcaattaac aactaccata agcttttagt tgaccaaaag gtaggctata gtgtcggaaa
     1501 tcccatagta tttaatgcag atgatgataa tctcactaag cttttaaatg acttactagg
     1561 agaagagttt gacgatacaa taacagaact atatctcaat gctagtaata aaggggttga
     1621 atggttacat ccatatatta atagaaaagg tgagtttaaa tatgtaataa ttccagctga
     1681 agaagcaatt cctatttggg atagtaaaag acagagggaa ttagttgcat ttattaggtt
     1741 ttattatatt gaagatatag atggaaataa aataaaaaga gttgagtact acacagaaaa
     1801 tgacgtaact tactttattg aaagaggtaa tagttttatt caagaatttt tatatgatga
     1861 atatggaaaa atgactgata tacaagaagg tcattttaga ataaataaca aagaacaggg
     1921 atggggtaaa gttccattta taccttttaa aaataatgaa aagtgtgtct cagatttaac
     1981 tttctataag tctttaattg atatatatga taataacatt tctacacttg cagacaattt
     2041 agatgaaatg caagaggtca tttatgtatt aaaagagtat ccaggaacaa gtctacaaga
     2101 gtttatagat aatataagat actataaatc aattaaagta gatggtggag gtggagttga
     2161 taaactagag ataaatatac cagttgaagc caaaaaggag cttcttgata gattagaaaa
     2221 gaatataatt atttttggtc aaggagttaa tcccgaatct caaaacacag gtgacaaatc
     2281 gggtgtagct cttaaatttt tatattcact acttgattta aaatgttcca agactgaaaa
     2341 gaagtttaaa aaagcaatta gagagctttt atggtttgtg tgtgagtatt taaagataag
     2401 tggtagtaag agctatgatt ataagtcagt tcaaataaca tttaatcatt ctatgataat
     2461 aaatgagtct gaaaaaatag atatggcttc taaatcaatt ggaattatat cagatgaaac
     2521 tattgttagt aatcatcctt gggttgaaga tgttaatgac gaacttgaaa gacttaaaaa
     2581 acaggaagaa acccaaaaag aatatgatga tttaattcct actaataatc aagagggtgt
     2641 tatagat
//
LOCUS       DAGQWR010000259         2578 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.264, whole genome shotgun sequence.
ACCESSION   DAGQWR010000259 DAGQWR010000000
VERSION     DAGQWR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.264"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            65..2410
                     /gene="secA"
                     /locus_tag="MIW95_003838"
     CDS             65..2410
                     /gene="secA"
                     /locus_tag="MIW95_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBY3153127.1"
                     /translation="MSVLDAILDKADEQEIKKLNLIVDKIESLEKDMEIKENEQLKEM
                     TNTFRLRLDKGESLDDILPEAFAVVREVSKRVLGMRQYKVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYEFLGLTVGVILSNQD
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEIDSILIDEAR
                     TPLIIAGDGDEDLKLYELANNFVKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNRPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEVISKAGKLDAITIATNMAGRGTDISLGAGDKEEEEKVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIKEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
BASE COUNT         1070 a          260 c          511 g          737 t
ORIGIN      
        1 gtctactatt ctataatttt aaggtataat aataattgta tgataaaaga ggaggtcaaa
       61 aaatatgtca gttttagatg caatcttaga taaagcggat gaacaagaaa ttaaaaaatt
      121 aaatttaata gtagataaaa tagaatcttt agaaaaagat atggaaatca aagagaatga
      181 acaattaaaa gaaatgacaa atacttttag acttagatta gacaaaggtg aaagtttgga
      241 tgatatactt ccagaagcat ttgctgttgt tagagaggtc tcaaaaagag ttttaggaat
      301 gcgtcaatat aaagttcagt taattggagg tatagtaatt catcaaggaa agatagctga
      361 gatgaagact ggtgagggga aaactttagt tgaggtagct ccagtatatc taaatgcttt
      421 aacaggaaaa ggtgtacatg taattacagt aaatgattat ttggcagaac gtgataaaga
      481 acttatgcgt ccagtatatg aatttttagg attgactgta ggtgtaattt tatctaacca
      541 agacccaagt ataagaaaag aacaatataa atgtgatata acttatggaa caaatagtga
      601 atttggattt gactatttaa gagataatat ggtgccagat ttgtctcata aagtacaaag
      661 ggaactaaac tttgccatag tagatgagat agactcaata ttaatagatg aagctagaac
      721 tccacttatt attgcaggag atggtgatga agatttaaaa ctttatgagt tagcaaataa
      781 ctttgtaaaa actgttaaag aagaagactt tgagatggat agaaaagata aaactatagc
      841 attaacagca agtggtataa gcaaagctga gtcatttttt ggtataacaa accttactga
      901 tataaagaat atagaattat atcatcatat aaatcaagct ttaagaggtc ataagcttat
      961 ggaaaaagat gttgactatg ttatctcaaa tggagaagta atgatagttg acgaatttac
     1021 aggaagagta atggatggta gaagatatac agatggactt caccaagcta tagaagccaa
     1081 agaaggtgtt gagataaaga atgaatctaa aactatggct actgtgactt atcaaaattt
     1141 cttcagacta tatgaaaaac tttctggtat gactggtact gcaaagacag aagaagggga
     1201 atttgagtca atctataaat taaatgttgt ccaaatacca actaatagac cagttattag
     1261 agctgatttg catgataagg tatttaaaac agaagaagaa aagtatagtg ctgttgtaga
     1321 agaaataata aggatacata agactagaca gccaatactc gtgggaacag tttctgttga
     1381 aaaatctgag aagttatcta aaatgcttaa gaagcaaggt attaagcatc aagtcttaaa
     1441 tgctaaacaa catgataaag aggcagaggt aatttctaaa gctggtaaat tagatgctat
     1501 aacgattgct acaaatatgg caggtagagg tacggatatt tctctaggtg caggagataa
     1561 agaagaagaa gaaaaagtta aaaatttagg tggtctttat gttataggaa cagaaagaca
     1621 cgaatcaaga agaattgaca atcagcttag agggcgttct ggtcgtcaag gagacccagg
     1681 tacatcaaga ttttttgtaa gtcttgaaga tgatgtaata aagctttatg gtggaaaaac
     1741 tatagagaaa cttatgaaga gaacaagttc aaaggaaaat acggctattg aaagtaaagc
     1801 acttacaagg gctatagaaa gagcacaaaa aggtgtagaa ggtaagaatt ttgaaataag
     1861 aaaaaatgtt cttaaatatg atgatactat taatgagcaa agaaaggtta tatataatga
     1921 aagaaataaa gtgttaaatg atgaagatat aaaagaagat attcaaaaaa tggttaaaga
     1981 catcatacaa gaagcaggag aaacttattt aattggaaga aaaagagatt attatggata
     2041 ttttaaacat ttatatagta catttatgcc tgcagataca ttactaatac ccggtgtaga
     2101 taaaaaaagt gtccaagaga taattgatag cacatatgaa atttcaaaaa gggtttatga
     2161 cttgaaaaag atgatgattg gtattgataa agttgcggag ttagaaaaaa cagtactttt
     2221 aaaagtggtt gatcaatact ggatagatca tatagatgct atggaacagt taagacagta
     2281 tataggtctt aaatcttatg ctcaaaaaga tccgtttaaa gaatatgctt tagaaggata
     2341 tgatatgttt gaagctttaa ataaaaatat aagggaagca acagtacaat acttatataa
     2401 atttaactaa aaaagttcat gtaattttta ttaaatgaaa aataaaatat tttgattctt
     2461 tatattgtaa ttacaattaa ataattaagg aatacaaatg aaaaagaagc tgaaaatcag
     2521 cttctttttc tgtgtgagaa atacggaaaa aagagatgct tgaaatttct ttcttcca
//
LOCUS       DAGQWR010000260         2573 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.265, whole genome shotgun sequence.
ACCESSION   DAGQWR010000260 DAGQWR010000000
VERSION     DAGQWR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.265"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            152..1864
                     /gene="ptsP"
                     /locus_tag="MIW95_003839"
     CDS             152..1864
                     /gene="ptsP"
                     /locus_tag="MIW95_003839"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBY3153128.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASIHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT         1030 a          247 c          464 g          832 t
ORIGIN      
        1 tttaatcata aattcagagt aaaaatacag taacaaacag tttataaaat ttaataaaaa
       61 attataagta aataaaacta taaaaatgta aattatttat ataaatttat agagtttatg
      121 tgctagatga tattttttag gaggtagggt tatggcttac aaaggaatag gtgcttctcc
      181 tggtgttgct ttagggaaag cgttagtagt tgaacatagt gaattagtaa ttgaaaaaaa
      241 atctatagat aatgttgaag ctgaaattgc taaattagaa gatgcagttg cagtttctaa
      301 agaagaatta gtaaaagtta aggaaaaggc ttctgaagaa ttaggagcag aggaagcaga
      361 aatatttgaa gcacatttat tagttttaga agacccagag ttaattggtt ctgcaataga
      421 taaaattaaa actgaaagtg taaatgcaga gtatgcttta aatgaaataa aagagatgtt
      481 tgttagcatg tttgagtcta tggacaatga gtacatgaaa gaaagagcgg cagatataaa
      541 agatgttaca aatagaatac ttagacatat attaggaatt aaagttgttg atttatctgc
      601 tttaagcgaa gaagttgtat taatagcaca tgatttaact ccatcagaca cagcaactat
      661 gaataaaaaa atggttttag gatttttaac tgatataggt ggaagaactt ctcatactgc
      721 tatcatgtct agaactttag aaatagctgc aatcgttgga ttaaatgata taacttcaaa
      781 agttaaggat ggagattttg tagttttcaa tggagacaca ggtgaagtaa ttgtgaatcc
      841 tgatgaagag actataaata aatatactga attaaaagct aagtatgaag atgagagaaa
      901 agcattacag ctattaaaag gaaaaccatc tgtaacttta gatggaaaac atgtggaatt
      961 agctggtaac ataggaacac ctaacgatat agaaggtcta ataaagaatg atgctgaagg
     1021 tgttggtctt tatagaacag aatttttata catggataga gattctttcc caacagaaga
     1081 aatacaatat gaagcataca aagctgtatt agaaggaatg gatggaaagc ctatagttat
     1141 aagaacattg gatataggtg gagataaaga attaagttat ttaagtatgg aacctgaaat
     1201 gaatccattc ttaggttaca gagcaataag actttgccta gatagaaaag atatatttaa
     1261 gactcaattg agagctttat atagagctag tattcatgga agattaagaa tcatgttccc
     1321 aatgatttca tctttagaag aattgttaca agctaaagaa gttgtaaaag aagttttagc
     1381 agaacttgat tctgaaggtg tggcttatgc taaagatgta gaaattggta tgatgataga
     1441 agttccatct gctgctgtaa tatctgatgt attggctaaa cacgtagatt tcttctctat
     1501 aggaactaat gatttaattc aatacacttg tgcagttgat agaatgaatc aaaagataag
     1561 ttatctttac aatcaattta atccagctgt tttaagactt ataaagactg ttatagacaa
     1621 tgcacataaa gaaggaaaat gggcaggtat gtgtggagaa tctgccggag accaaaaaat
     1681 gataccaata cttttaggta tgggattaga cgagttctct atgtctccaa tatcaatact
     1741 tcctgctaga aagttaataa cttcagttaa agagtcagat atgaaaaaat tagctgatga
     1801 tgtacttaat atgggtactg ctgaagaaat aaaaagctat atagaaaaga cttttaatat
     1861 ataattaatg ttatatagtt actaaaattc atttttctaa ataacaaaat tatattttac
     1921 gaattaaaaa ggagatgttt caaaataaaa atttgaagca tctctttttt ataaaagatt
     1981 actaagaatt acttaatctt tgtaatagtc attttgtgaa aaagcaaaac tgcattgtga
     2041 ctatagtata tatggatgtt attatcaata aaagtactat taaaatgaag attcattctc
     2101 aaatgatatc aaaagtagtt gtgatggagt aagataatat tttgtgattt aatagtaaaa
     2161 attaaaaaaa tatggtattg aaaaataatt aaaagtattt aataatagaa ttagggaaga
     2221 ataagtttat aagtaaaaat aattgattac tagttaataa aataaatgtg atatccaaat
     2281 aaatcctaaa tctaatttga gttttaatat agatagtcta aagtttgatt attaaagatt
     2341 taattgtaag ttgttacagt tgatgatatt gattagagat taaagtttag agtagaaagt
     2401 taccttaaat ttaaaaatgc aagataaccc aatctaaaaa ttataatcac tcgataggtt
     2461 taaggattta tttgtttatt ataaatagga gggtgtctca aaatgaactt tttagtttat
     2521 gaggcactct cttttatata gaattaaata tattttatta tttcaacaat gaa
//
LOCUS       DAGQWR010000261         2545 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.266, whole genome shotgun sequence.
ACCESSION   DAGQWR010000261 DAGQWR010000000
VERSION     DAGQWR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.266"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(218..1129)
                     /locus_tag="MIW95_003840"
     CDS             complement(218..1129)
                     /locus_tag="MIW95_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBY3153129.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SVGFPLYSKVGAAGGYHVLKERILPPITFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENMRRKLVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSIRTIVPIGLYAMNGLWYCPAYCIKSESVKEFRADRIVKILSIENLSNKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKK"
     gene            1240..1707
                     /locus_tag="MIW95_003841"
     CDS             1240..1707
                     /locus_tag="MIW95_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153130.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIEESYNFAKQKFKKRKNK"
     gene            complement(1875..2474)
                     /locus_tag="MIW95_003842"
     CDS             complement(1875..2474)
                     /locus_tag="MIW95_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153131.1"
                     /translation="MKLKRTLFVGLALVLAIGITACARDNEKPNENAGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKRMNDDLIAEGERLVKDIDERIEKLDKVSDEDMKKGQN
                     DFMKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT          841 a          421 c          304 g          979 t
ORIGIN      
        1 taggatttaa ccttatacca tctaaccaat tttttctact ttcgtaactt tcacacttag
       61 gtttatttca tccttatgtt ttagtttggt tagctttagg ggtttttagc aattcaagta
      121 gtattggata ggtgttatcc tatctctaca tataagtttc cctatatgct gactaattta
      181 gctactaact catgactaaa gtcacaagtg tgcgtagcta ttttttaatc aaatgtttaa
      241 gttctatagg ttctattatt gtagcatttt ttccaaaagt taaaaaatat tcagccaccc
      301 aattaagtgt agattttggt atatacatat ctatgtatcc acccttagac aaaattttca
      361 atcctcttgc caacaaaaat tctgtttcac aacgttttac tccctcatct gtcagattaa
      421 ttttaatatg ataatctgta cctacttcca tattcttaag ataatcatgt atagaagata
      481 aaaccttata tttcttattt gataagtttt ctatactcaa aatcttcaca attctgtctg
      541 cacgaaattc cttaacagat tctgatttta tacagtaagc tggacaatac caaagtccat
      601 tcatagcata caaaccaata ggaacaatgg tacgtatact atttttttgc tttgatgaat
      661 attcaatttt aattgtaaat tcattcatta ctatattaaa caattccttc aataatggtg
      721 ttgagcaatg cctatctggt gtccaaaaaa caagtttacg tctcatattc tcaatattat
      781 attgaatatc atttggtata caatttaaaa atttctttaa aacagaaata gtttcttgct
      841 caaatggtaa atcattataa tactctagtg actgatatgc aaagaatata gattttacct
      901 cactctctga aaatgtaatt ggtggaagta tacgttcttt aagtacatga tatcctccag
      961 ccgctccaac ttttgaatat agtggaaatc caacactttc caatacctgt aaatctctta
     1021 gtatagtacg ttttgataca gaaaattctt ctgctagctc tcccgctgta aaacttcttt
     1081 ttgcatttat tgttatcatc agttcaatta aacgctcagc tcttgacata ttcatactcc
     1141 taatttaaat atagtgacaa taattgtcac cattatatga taagatattc ttgcatctaa
     1201 agcaataata atattaaaga aagaagggat tgatatttta tggaaaatga tactattaag
     1261 gctgatgata ttctcaatta ttgtctatca aacttagatg atgttgtact aatggatagt
     1321 tggggggaac gagcaattta ctacaatcct aatggtgttt taaagcgagg ggtatatgtt
     1381 cttaccatta aagaaaaaga cagtaatcat gataaaggtt cgttggttag tcgtccaaat
     1441 gtataccgtg tgaatatagg attaaaaaaa gaaactttta ttgaaatgtt tggatatatt
     1501 ccaaagcgtc caggtgtagg gcaaatagtt gatatggatt ttgattttac aaaattggac
     1561 acaatcatgc cacatcctat ctactcatgg atgggatgga tatgcgtcct aagccctact
     1621 gaaaagacat ttgagaactt taaaacttta atagaagaat cttacaattt tgcaaaacaa
     1681 aaatttaaaa agagaaaaaa caagtaaaaa cataaaaaaa taacctctaa ataaactata
     1741 atgaaaatat gaggttacaa gataaaaatt agtatcttga taacatacaa aaaagcgaag
     1801 ccaaaaaata tattaattac aaggcttcgc tatatttcaa atatttattt ctaactttaa
     1861 aactatttac tctattattt tttattattt cctgttattc ccaatctatc attcatatct
     1921 tttatcattt ttctaaaacc actaccaata tcgtttccaa catcttcagt tccatgaact
     1981 agtttcataa aatcattttg accttttttc atatcttcat cagatacctt atctaatttt
     2041 tctattctct catctatatc ttttactaat ctttcccctt cagctattag gtcatcattc
     2101 attctcttta actctgtatc ttctgtttta ccgtcttttt taagagatgt tacaaatgat
     2161 tgtattttat ctctactatc tttatatgca gcctttacat cacttagata tttttcattt
     2221 cctggatatt ctttatcttt aaactgattg tttatacttt ctggagtatc ataaatagaa
     2281 tatctattta gacctgctat ataatcatta taacttgttg aataataatt catatatcct
     2341 tcattattat ttacagattc attaggattt tttcctgcat tttcatttgg tttttcatta
     2401 tccctagcac atgcagttat tcctatagct aatactaatg ctaatcctac aaataaagtt
     2461 ctttttaatt tcatttttat taccctccta gatgttttgt actacaaaaa attttaataa
     2521 ttttctttct taatttagta tttct
//
LOCUS       DAGQWR010000262         2412 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.267, whole genome shotgun sequence.
ACCESSION   DAGQWR010000262 DAGQWR010000000
VERSION     DAGQWR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.267"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            69..881
                     /locus_tag="MIW95_003843"
     CDS             69..881
                     /locus_tag="MIW95_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153132.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFIFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVLEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            892..2160
                     /locus_tag="MIW95_003844"
     CDS             892..2160
                     /locus_tag="MIW95_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153133.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNKNPKSRIRNDYLNIDKKYSDFVNDKG
                     KELIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     YQQNINEIKFSIGSKLYLEIVNNKSKNGDIEGHDSYSNLIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMIKNNKLNLIVTDMSNSIINLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGMKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT          990 a          178 c          359 g          885 t
ORIGIN      
        1 tatgaaggag aattacaatt taacagttca aatagtatgt tagaatttga aataaagtag
       61 gtgagttgat gaaagattta ataagattat tcgaatttga attaaaaaga aatataaaga
      121 attatatatt tataataatt tgttgttgtt cttttgtaat tttaaacata gtaaaaaatt
      181 taaatgacta taattacata atagaaaatg cagttaaaag tgaacagtta acaaaaatcg
      241 gaaatacgat agaaacggta aatgttacag gtttctcaag ctttagaaat ataatgggtt
      301 ctacagaatc gtggtttata tttggtataa ttgcatgtat ttgctatgct ttttttattt
      361 ggtatagaga ctttaatggt aggagtaaat ctatatatac acttataatg ctacctaaaa
      421 atagaatcaa tatttatata tctaaattat taaatatatt atttctagtg tatagctata
      481 cagtagtact tacaatatct ttgtttattg caagtaagtt gttgcctaga catatgcttg
      541 gtaatgtcac aaactatggt tttgtacaag aaacaatata tgagttaaaa atgttacttc
      601 catatagctt tgaaatacta tttgtagagt atatattttt attgatagga tttgtatctg
      661 tagtatttac atcaattctt ataaataaat ctatttataa agtaagcact ttaataagtt
      721 ttctattttt agttttggaa attttagtgt ttataatttc tatagaattt attatacctt
      781 atgaatacag tgatgtattt actgttctat attcaagtat aaacatattt gtatgtagtc
      841 ttatatctaa taaacttttg aagcaaaaga ttgactttta ggagggatat tatgaaagca
      901 aaaaatatag gaaaatatac aatatgtttt ataatagtat gttttttaat gattactatg
      961 accttaatat tgagttattt taataaggat aaactatctg taaaaacttt aaaaggcaat
     1021 gtaaaagata tagggagagt aagtgtagta tatacaccta atataagcaa attttcttat
     1081 gaggaggtaa ttataagtaa aaatggagta agaaataata aaaaccccaa atcaagaatt
     1141 agaaatgatt atttaaatat tgacaaaaaa tacagtgatt ttgttaatga taaaggaaaa
     1201 gaacttatat ataaagatga tttgaatata ggagaagcgt atacctctgt taagtctgat
     1261 ttagtacaag gaatgaatgc tttaaatgat tgtataaagg tttcaaataa aaatttaaaa
     1321 agtggggtag aagaaaattt tgatattaat ttaaataagt ctgattttaa agaaaacagt
     1381 tggaaaacct tgtttaataa aatatataaa ggcaatttat atatggtaat tagtgaaact
     1441 aatatttcag gttctaaggt caataatatt tgtatattaa agatagatat aaaaaataag
     1501 tcttataaat tattaaatag atttaattta ggattacaga atggatatca acaaaatatc
     1561 aatgaaatta aattttctat tggaagtaaa ttatacttag aaatagtaaa taataaaagt
     1621 aaaaatggag atattgaagg acatgatagt tactctaacc ttattatata tgatatagaa
     1681 gataacactt ttaatataag taataaattt atggataatc taaacaaaga aaagaatgca
     1741 aatccaaagg agattggctt attaagtaaa actaagctag attatatgat aaaaaataat
     1801 aaattgaatc ttatagttac ggatatgagt aattccataa tcaatcttgt ttatgatatt
     1861 aattctaatg agattaaatt agaagattat gagatacttg atttaaaagt agattataat
     1921 gaagcatcat ctggagttag gaaagttaag ttactggatg ataagattta ttctatttca
     1981 ggtatgaaga acagtatatc aggagaatta aaaggattta aagggattgt tgatgtagtc
     2041 ggaactaagc cagtaaaatt tcaagtattt gatatgaaac ttaaaaagaa tatttatgaa
     2101 gctgagctaa taaatggaga tataagttta caagaaaagt tattttttgt tgcaaattaa
     2161 gtattagaat taattttaga actttaaact tttatatgta tcacagataa gtataagtaa
     2221 gggagtgtct caaaataaac tttttagttc atgagacact ttttttatat gaaatcaaat
     2281 atattttcat tgtttagatt tattttgata atgaaaaaaa gattatcttt attttaagaa
     2341 agacatttat ttataattat atacaaaaat attttttaat gtttttgtgt ataattataa
     2401 caatttaatt ta
//
LOCUS       DAGQWR010000263         2402 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.268, whole genome shotgun sequence.
ACCESSION   DAGQWR010000263 DAGQWR010000000
VERSION     DAGQWR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.268"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            236..883
                     /locus_tag="MIW95_003845"
     CDS             236..883
                     /locus_tag="MIW95_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3153134.1"
                     /translation="MNILIADDEISMLKILCAYFKKENFNVFTAKNGEEALDIFYENK
                     IDLAILDWMMPIIDGIEVCKEIKENSNTRVLMLTAKSQSEDEIEALNIGADEYIKKPF
                     DPRVLIIRAKKLINYKNTINIKDLKIDFGSSKVFKGDKELLLTKKELELIRCLINNKG
                     MVLSREKLLDLVWGLDYEGDFRTVDTHIRRLRVKIGEDIIKTYRGLGYSLEDFND"
     gene            876..2270
                     /locus_tag="MIW95_003846"
     CDS             876..2270
                     /locus_tag="MIW95_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3153135.1"
                     /translation="MTKLNKKLSISISIVVVVVYIISIIINSMFIHRYYLHEKRNILD
                     NVENEIKDKDIDKLKSDITLIEEKNNIDIVYIELDKNHKDKGNIDKINQDLLNAFWKK
                     GLSLNKFWIEENSLNNIDKKSINKIYNQGKTKYSLLVKFIKKDNYLFAISIPIEHSEE
                     TIGIVNRFNIIMGIFSVVIITILTFILSNRVIKPMERLKFLSKDISELNFRTEDIKTN
                     DEIEELAYSINIMSEKLEKAHNELNKRNENLKTFISDASHEMKTPIALIKAYAIGMKD
                     GLDDGTYTDTIIEQAENMTNTINSLLYWAKYEKKERSLCLVDLKERLYKSLKNYELLI
                     ERGSISVKCSVDDKELIISSDEDSIDMVFNNLISNAIKYTNNNEIEINLFEENERIIL
                     SIKNGIDYNMGDDIENMWKPFYVLEKSRSKELSGTGLGLTIVRTILEENNFRYRVEIC
                     DGIIEFYIIFIKVI"
BASE COUNT         1053 a          164 c          395 g          790 t
ORIGIN      
        1 aaatattaag taatttcaaa aaaatataaa tttatttaat ttaattaaaa tattataata
       61 tattagttac atattaaaat atataatcga gatattgtta agacattgtc gagatattat
      121 aatatataac taatgtatta taaatagttg taaacttcct gtaacatata ttttatataa
      181 ttaaagtaat ggatataata tgaaatataa taatgaattt tagggatgtg aataaatgaa
      241 catattaatt gctgatgatg aaattagtat gcttaagata ctttgtgcat attttaaaaa
      301 agaaaacttc aatgtgttta ctgcaaaaaa tggggaagag gctttagata ttttttatga
      361 aaataagata gacttagcta tattagactg gatgatgcca ataattgatg gaatagaggt
      421 ttgtaaagag ataaaggaaa atagtaatac aagggtactt atgttgactg ccaaaagtca
      481 aagtgaagat gaaatagaag cactcaacat tggagcagat gaatatataa aaaaaccatt
      541 tgatcctaga gttttaataa taagagctaa gaaactaata aattataaaa atacaattaa
      601 tattaaagat ttaaaaatag attttgggtc aagtaaagtt tttaaaggtg ataaggaatt
      661 attattgact aagaaagaat tagaattaat tcgttgttta atcaataata aaggaatggt
      721 tttatctagg gaaaaacttt tagatttagt atggggattg gattatgagg gagattttag
      781 aacagtagat actcatataa gaagattaag ggtaaaaata ggagaagata taataaaaac
      841 ttatagagga ttagggtata gtctggagga ttttaatgac taaactaaac aaaaaattgt
      901 ccataagtat atctatagta gttgtagttg tttatataat atctataatt ataaatagta
      961 tgtttattca tagatattat ttacatgaaa aaagaaatat attggataat gttgaaaatg
     1021 aaattaaaga taaagacata gataaattaa aatcagacat tactttaatt gaagaaaaaa
     1081 ataatataga tatcgtatat atagaattag ataaaaatca taaagataaa ggtaatatag
     1141 ataaaataaa tcaagattta ttaaatgcat tttggaaaaa aggtttaagt ttaaataagt
     1201 tttggattga agaaaatagt ttaaataata ttgataagaa aagtataaat aagatataca
     1261 atcaaggtaa aacaaaatat agtttgcttg taaaatttat caagaaggat aattatttgt
     1321 ttgctatatc tatacctata gaacattctg aagaaactat tggcattgta aatagattta
     1381 atataataat gggaattttt tcagtagtta taataactat acttacattt atattatcaa
     1441 acagagttat aaaacctatg gaaaggttaa agtttttatc taaagatata tctgaattaa
     1501 attttaggac tgaagatata aaaacgaacg atgaaataga agagctagct tacagcataa
     1561 acataatgag cgaaaaactt gaaaaagcac ataatgaatt gaataaaaga aatgagaatc
     1621 taaaaacttt tatatcagat gcatctcatg aaatgaaaac tccaatagca cttataaaag
     1681 catatgcaat aggtatgaaa gatggtttgg atgatgggac ttatacagat acaataattg
     1741 aacaagcaga aaatatgacc aatacaataa attctctttt atattgggca aaatatgaga
     1801 agaaggagag aagtctgtgt ctggtagatt taaaggaaag gttatataaa agtttaaaaa
     1861 actatgagct gttgatagaa agaggtagta taagtgttaa atgcagtgta gatgataaag
     1921 aacttataat aagttcggat gaagatagta tagatatggt atttaataat ttgattagta
     1981 atgctataaa gtatactaat aataatgaaa tagagattaa tctttttgaa gagaatgaga
     2041 gaattatatt atccataaaa aatggtatag attacaatat gggagatgat atagaaaata
     2101 tgtggaagcc tttttatgtt ttagagaagt cgagaagtaa ggagctttct ggtactggtc
     2161 ttggtcttac catagttaga actatacttg aagaaaataa ttttagatac agggttgaaa
     2221 tttgcgatgg aattatagaa ttttatatta tctttataaa agtaatttga attaaaatgt
     2281 aaatataaag aataattaaa aaaatatcgc aattatttgt ttttcatatg gataaaattg
     2341 gttatttaat atataattac aagtataaat aataaaattt atttgtaaaa agagtagcac
     2401 tt
//
LOCUS       DAGQWR010000264         2350 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.269, whole genome shotgun sequence.
ACCESSION   DAGQWR010000264 DAGQWR010000000
VERSION     DAGQWR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.269"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            357..1976
                     /locus_tag="MIW95_003847"
     CDS             357..1976
                     /locus_tag="MIW95_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBY3153136.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGNCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
BASE COUNT          873 a          287 c          334 g          856 t
ORIGIN      
        1 aagtttaata accttacacc aattgaatat aaaaatattt ataactaaaa aatatcattc
       61 taaaaggatg ctattttttt acatattttt tacaattata gttaattctt tgttaaattg
      121 attgtacata aaatactaaa aatctaagtt caagtattta aattgctata tatcataaat
      181 tttctaaaat taacaacaac ttaaaaattt aacattctct taacgtatac ttaacaaaac
      241 attaatattt ctattgacaa aatttgttat tctatattat ggaaacaata aattttagtt
      301 aataaagttt ataattcaga taaaaaaact attaatttct taagggagga aatatcgtga
      361 atatagtaat tagccttatc ggtggacttg gactttttct ttacggaatg agtttaatgg
      421 gagaaggttt acaaaaatca gcaggagaca aattaaaaaa aattattgaa ctcttaacaa
      481 gtaatgttgt tatgggtgta ctggtaggta cagtcgttac aggtattata caaagttcaa
      541 gtgctactac tgttatggtt gtaggatttg taaatgcagg tataatgaat ctaagtcaag
      601 ctattggtgt aataatgggg gcaaatatag gtactacagt tactgctcaa ttagtttcat
      661 ttaatcttga aggaattgca ccaattgctt taggtatagg tattttattc tatttattta
      721 catcaaatca aaaaacaaag catttatctg agatacttat aggttttggt atattattta
      781 caggaatgga gtttatgaaa gatgcagtag ctccacttgc tgaatataaa gcattcactg
      841 atgcactttt atattttagt aaaaatcctg tattaggtat attggcagga tttgccataa
      901 ctggtattgt tcaaagttca agtgcatcta tgggtatgtt aatagcacta gcttctcaag
      961 gaatacttcc tctatcatct gctttaccta ttctttatgg agataatata ggtacttgtg
     1021 ttacttcact tttatcaagt gttggtgcaa gtagaaatgc tcgacgtgca gctgtaatgc
     1081 atttaagttt taatgtcatt ggtacaataa tatttatgct tgttttaaat aaacctatat
     1141 cagccatagt tactcatttt gacccaactg atacagctag acaaatagca aatgcacata
     1201 ctttatttaa tcttacaaat gttataatat tattaccatt ttctaaatac atagttaaat
     1261 tagcaaatag acttatacct attaaagaaa ctgaatcaga aatagtaaat aatactaagt
     1321 acttagatga aaggatgttt agcactccat ctatagcact tggtaatact gtacaagaag
     1381 ttgtaagaat gggtcataaa gctacaaact ctttggaaca ttcaattgca ggatttttaa
     1441 ataagagcaa tgaagatatt aataaagctt ttgagtcaga aaaagttgtc aataagttac
     1501 agaaagatat attgaactat ttgttaaaac tttctaaaga acctcttaga gacgatgaac
     1561 gctttagaac agatttgctt tttaatactg taaatgacat tgaaagagtc tctgaccatg
     1621 cagaaaatat agctgaattg gccatgagtg ttaaggaaat gaacattagt ttttctgata
     1681 gtgcgataag agaaatatac gaaatataca ataagacaat tactaatttt aaggatgcct
     1741 taatagtttt agatgttaaa gattttgagt tagcaaataa agttttagaa gttgaaaatg
     1801 aagtaaatta tttagaaaaa acttttagaa attcacatat gattagacta aataatggaa
     1861 attgtacaat tgatgctggt gtactttatc ttgacttact tactaatcta gaaagaattt
     1921 ctgaccactc aacaaatata gtaaaacaag tattaaaact taaacaaaaa atatagtgat
     1981 tatttttata tataatctta atcaatgaga agtaagaagc tatagataat gcttatatca
     2041 tttatggctt cttatttttt atgtaatact caaattttta tagtattctc ttattattaa
     2101 acagcttctg cctagaatat tccatatata tatgacttct ttcataaaac ttgaactgcc
     2161 cataactaaa gtcacaagta tacataacta ttttttaatc aattataacc aatatttatt
     2221 ttatttatct tcattttaat tcttcattca ataatgtaat aagattattt tttatttgga
     2281 tactattata tcatctaaat atgttattat atatttggat attttcatat taataggagg
     2341 gattctacaa
//
LOCUS       DAGQWR010000265         2233 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.270, whole genome shotgun sequence.
ACCESSION   DAGQWR010000265 DAGQWR010000000
VERSION     DAGQWR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.270"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            37..1857
                     /locus_tag="MIW95_003848"
     CDS             37..1857
                     /locus_tag="MIW95_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY3153137.1"
                     /translation="MTLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPEYLNVWELQ
                     GITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLRKTSAPITE
                     LTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPILLVEKNNV
                     PNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRYETSLLIAK
                     EIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTYKWLKSEDL
                     QNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFYTDDELEAV
                     LVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVYKIGGGLTS
                     KVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIKEKDYTLNT
                     SLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNASDTTGNGVE
                     VFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLYVLRNNDMP
                     AVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT          933 a          266 c          380 g          654 t
ORIGIN      
        1 agtgtcaagt ttaaagaaaa accagatgca gatagcatta cattaaagta taaatgctat
       61 gatatgccat tggatacaac tctaaattat aatcaatcaa ctgagtcata tgaaggaact
      121 atcaattata ataaagaccc agaatattta aatgtttggg aactacaagg gataacaata
      181 aacagcaaaa ataatcctaa aactttaaac aaacaagaac tagaaaagat gggattaaat
      241 ttaaaagact ataatgtaac gcaggaatgt ataattgaag atataacttc tagaaaagat
      301 gtaaataaat atttaagaaa aacttctgca cctattacag aacttacagg aagtgataga
      361 tacgaaacag cagttaaaat aagtaaagaa ggctggaaaa atggttcaga taaggtagtt
      421 ataataaatg gggatgtaag tatagatggc attatatcaa ctccactagc aactacatat
      481 aatgctccaa tacttttggt tgaaaaaaat aatgtaccta atagtgtaaa atcagaatta
      541 aagcgtttaa atcctagaga tgtaattata attggagatg agaatgctat ttctaaaact
      601 actgctaatc aaattaaatc gactgtaaat gctagtcaaa cacgtctaaa gggctctaat
      661 agatatgaga catctttatt gatagcaaag gaaatagata aaaatcatga tgtggaaaaa
      721 gtatacataa caaatgctaa tggcggagaa gtggatgcac ttactatagc agcaaaagca
      781 ggtcaagaca agcaaccaat tatattaact gataaaaata gtattacaga caacacatat
      841 aaatggttaa agagtgagga tttacaaaat gcttatttta taggtggacc tcaaatgata
      901 tcaacaaatg ttataaataa ggtaaatgat ataactaaag ataatgttac taataataga
      961 gtatatggag cagatagaca cgaaacaaat gcaaacgtaa taaaaaaatt ctatacagat
     1021 gatgagttag aggctgtttt agtagctaaa tcagatgtac ttgttgatgc tttagcagca
     1081 ggtccattgg ctgcgaactt aaaatctcca atacttataa caccaaagac gtatgtatct
     1141 gcataccata aagataattt agaagctaaa tcagctaata aagtatataa aataggagga
     1201 ggattgactt ctaaggtaat gaactctata gcatcatcat tatctaaaca caatacaact
     1261 ccaacagaac caggaaatag tgggggtaag acagttatga ttgacccagg acatggtggt
     1321 tcggacactg gaacaacagg taaaccatta gggggtatta aagaaaaaga ctatacatta
     1381 aatacttcac ttgcgacaac tgaatattta cgttcaaagg gattcaatgt aataatgaca
     1441 agagacacag ataagacttt atctcttgga aatagaactg ctttatctaa ctctttgaga
     1501 ccagatttat ttacgagtat acactataat gcatctgata caactgggaa tggtgtagaa
     1561 gtattttata agcttaaaga taaagatgga gggactacta aaactgtagc tacaaatata
     1621 ttaaatagaa tcttagagaa atttaatcta aaaaatagag gtgcaaaaac aagaacacta
     1681 tcaactgacc ctacaaaaga ttatttatat gttttaagaa ataatgatat gccagctgta
     1741 cttgtagaat gtgcattttt ggataatgag aaagatatga gtttacttaa tacatcaaat
     1801 aaagtaaaag aaatgggtac acaaataggt aaaggcatag aagattcatt aaaatagtct
     1861 acaatgagag catagaaaaa tttaaaataa attaatctgc aattttaaat ttaaactaaa
     1921 gctataagat aagaatgtaa ttataatagt aataaagact gtatatacat tgtgcatata
     1981 agtgcgaaat gaggtatata cagtcttttt atgattaatt gtaaaaggat ttatattata
     2041 tgaagaattt acatatctta aggctacagt taattgtcaa gagttggagt attcatttgt
     2101 aatacacaat aaaagcttaa ttactatatt ttattgtgtg tttgtgtgtt tgtgtgtttt
     2161 ttgtggattt ttatgtcaat aaatgatata gtatagataa ttaaatgtat taaatatatt
     2221 taattttttt att
//
LOCUS       DAGQWR010000266         2200 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.271, whole genome shotgun sequence.
ACCESSION   DAGQWR010000266 DAGQWR010000000
VERSION     DAGQWR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.271"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            266..>2200
                     /locus_tag="MIW95_003849"
     CDS             266..>2200
                     /locus_tag="MIW95_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBY3153138.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAITVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSSKVDKVVLKNTITAANKLYNEAIEGTNVGEYKVGSKAIYKTAIDKAQAI
                     LDKSGVTQKEVNDAVTALNTATDTFKAGKVVAVDKTALQDAVTAATALHAKATEGTAE
                     GNYAVGSKATYKTAIDEAQAI"
BASE COUNT          965 a          280 c          404 g          551 t
ORIGIN      
        1 caaaaccatg ttttttataa caattcatta acataaaaat caaacttacc ttaaaatata
       61 aatttacaaa tttttcaatc aataagaaaa tttctctatt ccttacataa tgactgtaat
      121 gtaacaaaat aattacaaat taattatgaa atcattatag ttagaaacct ctttaaaatt
      181 agatattaag tatcctttag aattagaacg ggaacaaagt taaaaaatta agtaatatca
      241 aatggacaag ggggaagaaa ataaaatgaa aataccaaag aagattgctg caatgcttac
      301 agttacaatg atagctggaa gttcaacagc aggcatagct agtgcacaaa ctgtggcaac
      361 aaatttaaca gggcaagaaa gatatgaaac tgctgttaaa ataagtcaag atggatggaa
      421 aaatgctgat gaggtagtaa tcgtaaacga ctcatctata gcagatgctc tatctgcaac
      481 accatttgca aaagcaaaaa atgctccaat acttttaaca agtaaagata aattaaatga
      541 taagacgaaa gcagaaatac aaagattaaa agctaaaaaa gtatacttaa taggtggaac
      601 ttctgtatta agtacaaata tagaaaaaga aataaaagat ttaaaaatat cttttgagag
      661 aatctctgga gctgagagat atcaaacatc cttagaatta gcaaagaaat tagatgctat
      721 aagtgatgtt aagaaaatag ctgtagttaa tggtgaaaaa ggacttgcag atgctgtaag
      781 tgtaggagca ccagctgcac aaaacaatat gccagtaata cttgctgatt ctaaaaatgg
      841 aacagcagtt gcagacaaat ttataaaaga tgctggaata actcaatctt atgtagtagg
      901 tggagagagt tcaatttctg aagctgttaa aaataaacta ccaaatagta caagactagg
      961 tggaacagat agaaatgata caaatgcaaa agtaataaaa gaattttata aaaagacaga
     1021 tttaaaaaat gcttatgtaa ctaaagatgg tatgaataaa caagaccaac ttatagatgc
     1081 actagcagta ggtgtattag gtgctaaaaa tcaatcacca gtagttttag ttggaaagaa
     1141 cttatcagct tcacaaaaat ctctagttaa ctctaagagt tttgataaga taactaaagt
     1201 tggtgggaat ggtaatgaga cagcatttaa tgaaatgaag tcattacaag aagtaaaaac
     1261 agttgaagct aaaacaattt ctgaattaaa atcagctata gataaagcta ctgctaatga
     1321 tgttataaac tttaagccaa ctagtgaggt taaagaagca tttacaatac aaactgataa
     1381 ggcgataaca gtaaacttaa atggtacata tacaaagact gtgactatca acatgcctaa
     1441 tggtgatgta aataactatg caaaagtgga tgatgtagtt atagatgatg taaaagatgg
     1501 aacttttgtt aactacggaa aaataactaa cttaaaggtt aatgataaaa atggagccaa
     1561 gatagaaaat aattctaaag gtgagattgg tagtttaaca gtagcatctg gagcaagtca
     1621 agtaaaagtg actaatggtg gtaagataac tacagttaca aataattcta aaggaactac
     1681 tatagataat aaaggtacaa taagtagtgt taagggagat aattctccaa caataagtgg
     1741 aaatagtcca agttctaact cttcaggagg aagttcatca agtggaggtt cttcacatgg
     1801 aggaggaagt tcttcatcaa aagtagataa agtagttttg aaaaatacaa taacagcagc
     1861 aaataaattg tataatgaag caatagaggg aacaaatgta ggtgaatata aagtagggtc
     1921 aaaagcaata tataaaacag caatagacaa agcacaggca atattagaca aatctggtgt
     1981 aacacaaaaa gaagtaaatg atgcagtaac agcattaaac acagcaacag acacatttaa
     2041 agcaggaaaa gtagtagcag tagataaaac agcattacaa gatgcagtaa cagcagcaac
     2101 agcattacat gctaaagcaa cagaaggaac agcagaagga aactatgctg taggatcaaa
     2161 agcaacgtat aaaacagcaa tagatgaagc acaggcaata
//
LOCUS       DAGQWR010000267         2167 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.272, whole genome shotgun sequence.
ACCESSION   DAGQWR010000267 DAGQWR010000000
VERSION     DAGQWR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.272"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            38..436
                     /locus_tag="MIW95_003850"
     CDS             38..436
                     /locus_tag="MIW95_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153139.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRRNARRHNIL
                     TKLVLSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    872..1135
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1286..1837
                     /locus_tag="MIW95_003851"
     CDS             1286..1837
                     /locus_tag="MIW95_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBY3153140.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          901 a          209 c          307 g          750 t
ORIGIN      
        1 taatgaacta gttaaaaatt tttatgaggg ggaaaatatg aataagaaga aaaaattgat
       61 tgggatagct atatcttttc tacttataat cagtattata aatccattac aagtagaagc
      121 agaagctatt attgagtcta gtgtatacat ttcttcaata aaagagaatt atacatatga
      181 taatactaaa tttaaaacta ttaaatctaa aagtagacat aaaagtagga aatataataa
      241 aagtaaagta aagttgaaaa aacgaagtat aagaactaaa aagtatggat atcaaactat
      301 aaataaatat aaaagacgaa atgcaagaag acataatatc ctaacaaaat tagtattgtc
      361 cataatagtc atatttttgg ttatagtttt agtagtgcta atatttatta aaagaaagaa
      421 gaaaaaattt tactaaaaat aattaaaaga atatcatttt tctaatcatt ggtatctatt
      481 taataatatg atgtaattaa aatggagtaa tattctgttt caaaggaggc atataaatac
      541 aggaatgagt attaaatgat ggttaattaa gtaaatttat tgataaatgt acttaattaa
      601 ctttgaagaa tttaattcct catgaatcaa aatataaaat tacatcaaca gttattcaca
      661 aaattaatat aagaagaatt agagccaaag agcagactat tagaccatta aagtaattat
      721 tcatagaatt ttgtatatga aaagaaatgt atagtataat ttactttttt tataaaaaat
      781 agattacaaa atatattttt tataaaaagg tattgacatt ttttagaaaa aagaatatca
      841 ttattttaac aacaaaattc aatacaataa attaaatcct atgacaagga agagtagtta
      901 aatttaggtt cagtatagag agctgaggat ggtggaatct tagcaagaaa aaatttgatg
      961 aatggccctt agaggaataa attgaaatca tagttttgag agtagacttt atcgtcagtc
     1021 gcacgttata gcgaataagg tatgtaagta cctgataatg agatgtatga aaattgaatt
     1081 ttatattaat ttttatataa tttaggtggc aacgcggata atctccgtcc tattagttta
     1141 ggacggagtt ttttatttta ctgaaaattt aaaagataga aggtggtgag aaagatgtta
     1201 tgaatttcaa aagctaaaga tgatgatata gtaggttggc aaaaaaataa attgaaaatt
     1261 aaaaaaatag gaggaagtac aaaaaatgaa aacaaataca aaaaaattaa ctttaaatgc
     1321 aatactttta gcgatgggat tattaataca tcaaatcaca ccagctatag gattaccaat
     1381 gaagccagat gtaccactag caatgttatt tgtaatattg gttttaaata gagatgatta
     1441 taaaacttgt ttgatagcgg gtatagtaac aggaatattt acagcactta catcaagttt
     1501 tccgggaggt caaattccta atgtaataga taaaactctt acagcaaata tagtattttt
     1561 attaatgaat attagttata atatgccttt tataaaaaaa ttagctaaga aaacacaaga
     1621 tactgtagta gttgcaataa taatgccaat aggaactctt gtaagtggaa ctatcttttt
     1681 attagcagct caagctatag ttggtcttcc aggagcttca tttacagctt tattcttagc
     1741 agtagtatta ccagctgttt taataaatct agttgcagga atatttttat tcaagattgt
     1801 tagtctatca ataagaagag ttagttatca agcttaacta cgaaataata ttttacttta
     1861 taaagcatta taataactaa ataacataag aataaagtag attataacta ttttattctt
     1921 atgttgttta aagatttggt attatatatt tatttttaat aaaatattta tatagtacca
     1981 attacactta atttaacaga taaattatca tacctatata atattcttat atgtatctca
     2041 tttgtagtta ttaaatataa gatatattta attgactttt caagataaat ttttatatta
     2101 atctcctctg atttctcatt ttttgaatac actaaaattt ccaattaaat caattatata
     2161 tttatag
//
LOCUS       DAGQWR010000268         2167 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.273, whole genome shotgun sequence.
ACCESSION   DAGQWR010000268 DAGQWR010000000
VERSION     DAGQWR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.273"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            245..511
                     /gene="rpsT"
                     /locus_tag="MIW95_003852"
     CDS             245..511
                     /gene="rpsT"
                     /locus_tag="MIW95_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBY3153141.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            complement(698..1741)
                     /gene="holA"
                     /locus_tag="MIW95_003853"
     CDS             complement(698..1741)
                     /gene="holA"
                     /locus_tag="MIW95_003853"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBY3153142.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(1695..>2167)
                     /locus_tag="MIW95_003854"
     CDS             complement(1695..>2167)
                     /locus_tag="MIW95_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBY3153143.1"
                     /translation="LATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIP
                     LLSIIGNLIIVPFVGVIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISN
                     LSFAYINIESPKFYIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
BASE COUNT          805 a          309 c          206 g          847 t
ORIGIN      
        1 tcaaaatatt tgttacaaaa ataaaatatt aatttatata tgtcaaaata tttaaaacac
       61 aaaatatacc aatttaaaaa caagatattg caaattattt atacacaatt tacagtctaa
      121 aaaatcaaaa taaaaaacat tattaaaata tataaataat aatccttgat ttttagattt
      181 atttctgtta aaatctaata tgttgacgat gaatatgata aaaaaataca gggaggtgaa
      241 cacggtggca aatataaaat cagctaagaa aagaataagt gttatcgaaa agaaaactgc
      301 tttaaataga gttagaaaat ctcaaataaa aactgcaata agaagattcg aagatgctgt
      361 tgcagctgga aacagagagg atgctgttgc aaagttccaa tacgctcaaa aaagaatata
      421 ccaagtagct tctaaaggaa ctatacataa gaatgctgca gctagaaaag tagctaaatt
      481 agctcaaaaa ttaaacggta tgaacgctta attaccagtt tatatttctt atatataata
      541 acccctatgt aggggttttt tattttgtaa atttatacaa taaaaaataa atagttagac
      601 atattatagt tgattgtata tcaaacttaa tttagcttac atctactctg aactataaca
      661 tatcttgcta tttatttttt attaatatat agatttttta aattgcttct cttttacagt
      721 atctactaac caacatttca actgctaaag tatctcttat aagacctgtt tttattttat
      781 aatcactttc taatatatag tttaatattt ccacaattat atcatcagaa aaatttttag
      841 tttgctttaa tgcttttcct acaacaaatt gatgcatttt taatttatca gctataagtt
      901 ttgttgaata tccatcaagt tgtaactgtc ttacttgcat aattatttta aattgtcttg
      961 ctatcataga aaatatacct aatacagatt cgccttcttg tatcatatca tttaatattt
     1021 tcattgcatt agaagcattt tgttctccaa tataatcgat taatttaaat atatcattct
     1081 caactttttt ttgagataat ttatcaatta cgtcattggt tacattattc cctttaccaa
     1141 caaaggaact tattttattt atttcatttt ctaaatcaga taatgtcttt tcactgctct
     1201 tatctctgta tccttcttgt tcaataaaat acatgatttg agaattatct attataacat
     1261 catttaaagc aaaagatttt tttatccatt taaataaatc catatcagaa agcttatcac
     1321 aatcaaaaac aattccattg tttcctattt tcttaactaa tgattttctt ttgtctacat
     1381 ctccatatac aacaaaaact atagttgtag tgtctggaat attatctaaa tgctctatta
     1441 agtatttctc atcactatct gtaaaattct ttttctttcc ttttaatagt tcgaagtctt
     1501 ttattattac tatctttcta tcatccataa atggaagtgt ttctatagag cttataagct
     1561 gatttaagac tatttccttc ccatctatta tatctaaatt aaaatcaagc attccttcgt
     1621 ttagactatc tttaaatgct tttattgcat tctcaattaa ataatactct cttccataaa
     1681 aaagatacat tttttcaaag tttttctctt ttacattcct tataatatcc ttgtaattca
     1741 ttctcttgct cctttatctc ttttccttct atgtaaaata tgtaaaaaaa cactcctatg
     1801 taataaataa caacaatata aaatttagga ctttctatat taatgtatgc aaaacttaaa
     1861 ttgctgattt tttctagtaa aaaaaatatg ctttctatca atgtactatt aaaaaaagat
     1921 ataattttag ctattactac acttacttta aatacgatta aacttgcaat acttaaatac
     1981 attattactc ccacaaatgg tactataatt aaatttccta taattgaaag caatggtata
     2041 cctttaaatg tataataaat tataggtaaa gttaatatat ttgatgatat tgtcaatgat
     2101 accatactta attttataat actatttatt ttattataga atattaatat agaaagtgta
     2161 gctaaaa
//
LOCUS       DAGQWR010000269         2076 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.274, whole genome shotgun sequence.
ACCESSION   DAGQWR010000269 DAGQWR010000000
VERSION     DAGQWR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.274"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            325..609
                     /locus_tag="MIW95_003855"
     CDS             325..609
                     /locus_tag="MIW95_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153144.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGRICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(766..939)
                     /locus_tag="MIW95_003856"
     CDS             complement(766..939)
                     /locus_tag="MIW95_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HBY3153145.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
     gene            complement(1379..1594)
                     /locus_tag="MIW95_003857"
     CDS             complement(1379..1594)
                     /locus_tag="MIW95_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3153146.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1605..2072)
                     /locus_tag="MIW95_003858"
     CDS             complement(1605..2072)
                     /locus_tag="MIW95_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBY3153147.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFILENVKRFKVMGCCLLINTIIECVIG
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          842 a          218 c          252 g          764 t
ORIGIN      
        1 atattaacta tatggaatgt aaagtctagt tttttctctt tagaaaaaat aaaaacaact
       61 gttttatatt aactatatgg aatgtaaatt aaaattgtgt tttctctttc atacacccac
      121 ttcattagtt ttatattaat tatatgtaat ataaatgttt tttcttatta aataaagatt
      181 gagtcacttt atatttaata atacatattc taaattgatt ttgtttttat ataagagaat
      241 ttgaataata tggaaaaaga tgattaataa attattaatt agggtataaa atgatataaa
      301 tagaataaat aaggggtgga tgaaatgctt gtatataata aaagttttta tcctaatgac
      361 atatttccaa gattagattt ttcaaaaata aaaaaacagt taaaattgat agataatgac
      421 ctgtcagatt ttggaagaat atgtataata gaaaaagaac attatacgat aagtgtaaac
      481 agtataggtg aaataaatgt gtattatgat ttagagtacg aaaataaggt gtatagaata
      541 gtttatgaga ttgaaaagtt atttaaatct caagttggaa ggtttagtat atctacatac
      601 agaaattgat aattaaaaaa gtagaaatta aaaaacttaa tactaaagat atagataaat
      661 atataaggta acagctagag agagttgtta ccctttttaa atactaagta tatattattc
      721 ttattaattt tttgtaaatt cactaaaaga cctttttata gaatattatt ttcttttttt
      781 attaaatagg taaatcagta aaaatgcaat tccagttcca attattattc caatgatatt
      841 ttctataatt aattttttta atataaaagt aaataataaa aatgctatac ataaacctaa
      901 aatatatcct ttaaaatagg ttgaaagttt tcttttcata taatcaccaa cttgtatcag
      961 atttagttta cttaattaga gtataacagt gttgtaactt caaagagtat ctaaaagatt
     1021 acaaatgtta gataaatcat caataatgta atttataaca cgtagctata aactatgcaa
     1081 taatataaat ggaggtataa gtaaatgaaa aagttccatg ttttgctaac agtttaaata
     1141 aatattaggt agaaaagaat gtatatagga atctgtggca aaattatcaa tttaaaagta
     1201 tgatttagtg tgatacagtt cttataggta atggataaca aatagaaaaa ggaacagttt
     1261 ctttcttaga acgtattttg ttatgacttt aaacaaagtt aatattaatg tcaataatat
     1321 agtaaaagaa taaaaatcat atagtataat aaaaaagagt gtttctaaac actctttttt
     1381 aatcattttc atcttctaca tattcaagta tatcacctgg ctgacagtct aaagctttgc
     1441 aaatttcgtt tagagtggta aatctaatag cttttgcttt tccagttttt aaaattgata
     1501 aatttgcatt tgtaatatct attttttctg ccaaatcttt aagtgatatt ttcctttttg
     1561 ccatcattac atctaggtta actactattg ccataacatc acctctatat cgttaaatca
     1621 ttatcttctt ttatttttat agctttatca aatgtttctg caattacaaa gcacattagt
     1681 gcaaatataa aagaaataac cataagtggt gagataccgc cactattatc tccaaatatc
     1741 attataagac tagtatttga gttataacca attacacatt caattatagt gtttatcaat
     1801 aaacaacatc ccattacttt aaaccttttt acattttcaa gtatgaatgg ggaatactta
     1861 gtactattaa ttataaatat caagaataag atgactataa agtagtcaga aaaatatagt
     1921 attcttgcaa ttaaagcgat tactttgtca gaactaaaat catataaaaa cataggtatc
     1981 atacataaga atattatacc agcaactcct aaaagtgata atagaaaata tagaaatact
     2041 gataatatat tactaaaaaa ttttgtaatc ataaaa
//
LOCUS       DAGQWR010000270         2022 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.275, whole genome shotgun sequence.
ACCESSION   DAGQWR010000270 DAGQWR010000000
VERSION     DAGQWR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.275"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            188..382
                     /locus_tag="MIW95_003859"
     CDS             188..382
                     /locus_tag="MIW95_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBY3153148.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIREFQDTIIL"
     gene            complement(484..993)
                     /gene="vanZ1"
                     /locus_tag="MIW95_003860"
     CDS             complement(484..993)
                     /gene="vanZ1"
                     /locus_tag="MIW95_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBY3153149.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1313..1703)
                     /locus_tag="MIW95_003861"
                     /pseudo
     CDS             complement(1313..1703)
                     /locus_tag="MIW95_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          744 a          269 c          198 g          811 t
ORIGIN      
        1 aattttataa tctattcaat aatatacttt tttctataaa tattttaccc ataaccttta
       61 tttttttatt aaaatgcatt ataataaaaa gtaaatctaa atttgataca gttttaacaa
      121 tcattatttt cagaaaagtg atactattga atatattaag aataattaga gatggaggaa
      181 ctaagatatg agcgtaattg taatgtgtag tcgttgtgga aaagaaatag ataaatatag
      241 aaataaatat ataacttatt atgagaaaaa taccaatgca gaaattaaag tttgtataaa
      301 ttgtgcatta gatttattag aaaaaacaag agaaaatcaa gaattgaaca tacgagagtt
      361 tcaagataca ataattcttt aataatttag gaaagcacct atataggtgc tttttatgtg
      421 taaataattt tatatgtaac tgtatcttac gttctatttt aagctaaacc tatatactta
      481 tttctataag tttgcaaaaa taactaatcc taaaaataat acaacaataa ttgtcacaat
      541 caatgccaat acattgataa tttttatggt attattctta aatattttcg ataatagcat
      601 aaaaacagct attcctatga ttcctcctag tgtgtttcct atcaaatcgg ttatatcact
      661 tgcacctagt gcaaatatat actgaagtgt ttcaaatgct aaactagtta taaaaatagg
      721 tataactttt tttataaagc tccattcttc ttttagcata cacacatata tcccaaatgg
      781 tacaaatata gccacattta atataatctc tgatatatct actctattat taattataag
      841 tgacccagca aaaggtacta agtttatgct tctaagattc atggtttcaa gagatgagat
      901 atcaaattgc aacttaaata gtattatcca tgttaaaata attatgtaaa ctataaataa
      961 tccttttgta atattatgtt ttctagattt catttattac ctccttacta tattattgta
     1021 ttattttatt aatacaataa tatagtaagg taatttatct gtcaactatt tcttctaaaa
     1081 ttatattcct tttataattt caatatcttt atcatattta catgatttaa gctattgtag
     1141 tatttcatat atctttaaat tttaattggt attattctct atatttttta ctatcaaaca
     1201 ctaaggaaat aatcactttt ctttatataa tgttttacca tcacataaaa ctaacattaa
     1261 atccaaacgc tactctcaat tgtctcaaac taaaaagata ctgatagctt tattagtcat
     1321 caatatcttc ttctttcaaa tttttaaagt attcttctaa tttgtccaag ctcttactat
     1381 ctaaaacttc atcatcatgt agtgctgaaa taaaactctt aaatgaattg ttatgcataa
     1441 aactaaaaaa actctttgtc tcaactttta agtaatcttt ctcttttata gctatagtat
     1501 aatgcgtaca tctatctacc ttaataagta gttaaaaatc ccttatctac taaagttttt
     1561 aaaaatgtca atgttgtgct ctttttccaa tcgtatttca acttcattgc ttctatgata
     1621 tctcttgagg ctaatacatc atctgctccc caaatatact tcattagttt tagttctgca
     1681 tgtggtaatt tattaatctt cattagttta cacctcttac caatttatgt ataattaaat
     1741 tttatactaa taatagtatg taatcaatca gattggaaca aatataatat tccaatttat
     1801 tccatataaa aataattaat aaaaaagaag tactctactt tagagagtac ttttggatta
     1861 tatattcaaa aatttatcta acatgattag agtatgtttt atgctttagt gtgataattt
     1921 tagttcgttt tattattata atatgtatta tatttcatta taatctataa gcttaaatac
     1981 aggatattta tttcatatac ttaatttata ttaagtttta tt
//
LOCUS       DAGQWR010000271         2000 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.276, whole genome shotgun sequence.
ACCESSION   DAGQWR010000271 DAGQWR010000000
VERSION     DAGQWR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.276"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            225..839
                     /locus_tag="MIW95_003862"
     CDS             225..839
                     /locus_tag="MIW95_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBY3153150.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            857..1219
                     /locus_tag="MIW95_003863"
     CDS             857..1219
                     /locus_tag="MIW95_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3153151.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYKGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1325..1822
                     /locus_tag="MIW95_003864"
     CDS             1325..1822
                     /locus_tag="MIW95_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBY3153152.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          762 a          222 c          330 g          686 t
ORIGIN      
        1 tataactcaa ttaatttttt aaatacaact ttataatata aaacttataa taaaagtaaa
       61 tttatatgtt aaatgaggtt gattttttat tattttttat ttatgctagt actaaagact
      121 tgcaaacgtt ttttgacaag tctttatttt gtctaaatta aacaaacttt tttacaaata
      181 aatacataaa atttaaacta atctatttat tggaggagat aaaattggaa gaagtaattg
      241 caaaaattat tactatagaa aatggattca aagaaattga aaacattgct aaaaaaattg
      301 taaaaaataa tgactcaaaa aagtgttatt atctatcaat ctacttatat aggtcagaat
      361 attatcaagt aagagaattg gctgtgttta tacttggttt tatatcagta actatttctg
      421 aagctttgtt atttttaaaa gacaatgtga gtaaagatga aaactggaga gtacaagaaa
      481 tactagctaa atcatttgat tattattgca gtgaagtagg atatgaaaag gcacttcctg
      541 ttataaaaga atggcttaat tctgatagtc caaacataaa aagggctgtg acagaaggtc
      601 ttagaatttg gactagtaga gactatttta aatctaatcc tgacgttgct atatcactac
      661 tttctagttt aaaagaagat gatagcgaat acttgagaaa gtctgtaggt aatgctctta
      721 gagatataag taaaaaatat ccagatttag taaaaaaaga attagattca tgggatatat
      781 caaataaaaa agttgagttt gtatataagc ttgcttcaaa atgtatcttg gcaaagtaaa
      841 atggaggtga acttatttga aacttcgtga tgagtatact tgcccattag aactaacaca
      901 tgatatgata aaaggcaaat ggaaatctat tatattatgg agattacgca taggaccaac
      961 ctccttatca aaacttgaaa aggaaatcaa tggtattaat caaaagatgt tacttgaaca
     1021 acttaaagaa ttaattgaat ttggtcttgt agataaaaag acatataaag gttacccact
     1081 taaagttgaa tactcactta cacctattcg agggaaacaa atattggatg ctttggctat
     1141 aatgcaagat ataggaatta gctttatgtt ggataatggt atggaagata ttcttaagga
     1201 aaaaggtgtt ttaaaataaa tagataccca caaaaaagta agtaatttac cattattttc
     1261 aactggtata taattaaatt atagatatat tctattaatt tattataata ggaggtaaat
     1321 tattatgaaa gtaactagta caggtatagt aaatggtgtt atagaagata aatacggtaa
     1381 aagaggaact cagttcaatg aaggtggtat gcctacatat tcactacctc taaagtttga
     1441 agatgctcca aagaatacag tatcctttgc tgtattttta gaagataaag actctgttcc
     1501 tgtatgtggg tttgcatgga tacattggtt agctgtaaat ataacaaaag atgagttaaa
     1561 tgaaaatgaa agtgtaacag ctactgattt tgtacaaggg actactagtt ggcatggcaa
     1621 actaggaggt cttgacagat tagaggccag tgtatatggt ggaatggctc ctccagacag
     1681 acctcatgtc tatgaaatac atgtatttgc acttgatact atccttgacc ttgaaaaagg
     1741 attctatatg aatgaatttt tcaaagctat tgaagggcat gttttagata catttacact
     1801 aaaaggaagc tattctaatt aattaggttt actatagcta atcattaatt aaatgctaca
     1861 tttaattgat gattagctta attgttatta gaatattttc tgcactttat cttaatatat
     1921 attttagttg actggtattt taatttctat tagattatta tcaatttata taaattgatt
     1981 ttagcttaaa acttacttat
//
LOCUS       DAGQWR010000272         1999 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.277, whole genome shotgun sequence.
ACCESSION   DAGQWR010000272 DAGQWR010000000
VERSION     DAGQWR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.277"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            439..1767
                     /locus_tag="MIW95_003865"
     CDS             439..1767
                     /locus_tag="MIW95_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY3153153.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGDKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGIKR"
BASE COUNT          714 a          219 c          346 g          720 t
ORIGIN      
        1 ttataataaa ttgtcaaatt atttattaat ataaaataga ctataaaaca agcaaaaatc
       61 atactattat ataaaagacc ttaaagatag aaaattacag aaagaagcca aaatgttttt
      121 gaaaaaaatg aaattttatt gcaaaaaaat caaaatcaat ttaacttaat agttataatc
      181 tatttttagt gttacaaata tacaatgtat attatacaat ataaaaaata cgtttattta
      241 taaatattat tatatttgga tttttggttt ttataatttt tttatattta aacataacta
      301 aaagtaaaag attacgaaag ctaagtagag tttattttat tttaataaat aaaaaagcta
      361 tttttctata tattggcata atatatgctt atttaactat gaaattaaaa ttaaatagga
      421 ttaatgagga ggaaaagaat gggaagtaat gaactgaaaa agactcttgg cgtatcagca
      481 gcattatcaa ctgttgttgg ttctgttata ggagcgggcg tattttttaa accacaagca
      541 gtctatacat taactggagg agctccaggt cttggtatat tggcatggtt aatagctgga
      601 atcataacta taacagctgg tcttacagct gcagaagtat cagttgcgat accaaaaact
      661 ggtggaatga tggtatatat aaaagaaatt tatggagaca aattaggttt tttaactgga
      721 tggatgcaaa ttgtattatt ttatccaggg atgatggcag cattaggtgt tatatttgga
      781 gaacaagcat cagcactaat tggtagccca tcacttttac ttccaatagc aattggaatt
      841 atagtaatag ttgcagggct taatatgtta ggttcaaaaa ctggaggagt catacaaact
      901 gtctctacaa tttgtaagtt aataccactt attttaataa tggttgttgg atttattaaa
      961 ggtggtggaa ataatccaat acttacacct atggttggtg aaggattaag cctgggtagt
     1021 gtactaggtc aagttttaat tgctatatta tttgcttttg atggatggat gaatgttggt
     1081 actttagctg gagaaatgaa gaatcctgga aaagaccttc caaaagctat aataggtgga
     1141 ttgtcagttg ttatggctgt ttattttata ataaatttag cgtatctttg ggtattacca
     1201 gctaatgaat tagcaaatta tgcttctcct gcatcagcag ttgctgaagt tatatttggt
     1261 tcaatgggtg gaaaaataat atctgtaggt atattaattt cagtgtttgg agcattaaat
     1321 ggatttttac taactggttc aagggtggca tatactttag ctacagataa gactttacca
     1381 aagtatggta tattttctaa attaaattct gctcaagttc cagcaaatgc aatagcatta
     1441 gtatctgtta ttgcatctat atatgctttg tcaggtcaat ttaatttatt aactgactta
     1501 gctgtatttg ctacttggat attctatgta ttaactttta taggggttat gaaacttaga
     1561 aagactcatc caaatatacc aagagagtat aaagttccat tatatcctat tgttcctata
     1621 atagctatag caagtggtat atttgttgta gttaatcaat tgtgttttgc agggatgaaa
     1681 actactatga tatctatagg aggtcttgta ataacagcta taggcttacc agtgtatgct
     1741 tatatgacta gaggaataaa aagataaacc aagtagttta caaatgcttt ataattaaaa
     1801 taaaagagac tgtctcaaaa tagagataag tctctttttt attgttaaaa gggtaaagag
     1861 atgttttatt ttataaaaaa agagtgttct atgaactaaa aagtttattt taaggtattc
     1921 actttatttt tagtttttga attataaaat taaacatcta aaaaattatt tgctttaaga
     1981 tactttattt taaagtgtt
//
LOCUS       DAGQWR010000273         1914 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.278, whole genome shotgun sequence.
ACCESSION   DAGQWR010000273 DAGQWR010000000
VERSION     DAGQWR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.278"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            138..1541
                     /locus_tag="MIW95_003866"
     CDS             138..1541
                     /locus_tag="MIW95_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY3153154.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
BASE COUNT          760 a          243 c          309 g          602 t
ORIGIN      
        1 attttttcat acatcttaca aatttatttt tatttagata acatgtatat tttttccatg
       61 aaaaataaat aataaaaaat agtaaatatg gaaaatataa aattgattaa caataattaa
      121 ttacctgagg tgtgaatatg aaaatgctaa acaacttttt taagaaaatc aacaatccag
      181 ctcctgtctt tgcacagaca aaaatggaaa gagataatca aacagatgat tcactttcta
      241 caaaaccaaa gactacattt agagacgttg ctggattaga cgaagtaaaa gaagaactat
      301 ttgaaatagt cgattttatg aagtcaccac aaaaatatca aaaaatgggt gcaaaaatac
      361 ctaaaggagt attattttat ggtcctccag gaacaggtaa aaccctactt gcatctgctg
      421 tagctggtga aacaaactca tcatttttca atgttactgg ttcagagttc gttgaaaaat
      481 atgtaggtgt gggagcaaaa agagttagaa ctctatttga aaaagctaga aaagaagctc
      541 caagtataat atttatagac gaaatagatg ctgtaggagc aaagagacat cttgaaagta
      601 acaacgaaaa agaccaaaca ctaaaccaac ttttagttga aatggatgga tttaataaag
      661 attccaatgt attaataata ggtgctacca atagacttga cttattagat gaagctttac
      721 ttagaccagg aagatttgac agacacattc atataggtgc accaaactat cacactagat
      781 ttgaaattct aaaggtacat acagatgaca aacctataga caaatctgta aatctagaac
      841 ttttagctaa aaaaactcat ggtttcaatg gggctcatct ttcaaatata gcaaatgaag
      901 cagctatatt tgcagttaga gatgacagtg agtgtattac atcagaacat tttgacaagg
      961 cacttgaaag agttattgca gggctagaat ctaaaaactc tgcattggtt gaaaaagaga
     1021 aaaaaatagt agcatatcat gaagctggac atgctcttgt aagtgatata gtaggaattt
     1081 gtcctataca aaaaatatct atagttccta gaggacaagc attaggttat gttcttcaat
     1141 tacctgatga agatagatat atttatacta aggatgagtt gattggaaaa ataaaaatat
     1201 tacttgctgg aaaggcttca gaagaattaa tatttaatca taaatctact ggtgcaaagg
     1261 acgacttaaa gaaagtcact gaaatagcta accaaatggt ttgtgaatat ggtatgagta
     1321 acttaggatt tatgactatt gatggaaatg ataagacttt cttatgtgat aaggttcaaa
     1381 aggaagcaaa tcgtatagtt gaaatttgtt ataaagagac tttagagatg ttaaaggata
     1441 atcttgaaga tttacattct gtttctaaat tcttatttga aaaagaaact atgactcatg
     1501 aagagttaaa agatttgata ggaaaagaag ctgtaaacta atatagtttt aaaaatttga
     1561 ggctgtctca aaatagagat aagcctcttt ttattgttaa agttataaaa atatatttga
     1621 ttttatacaa aaaagtgcct cataaattaa aaatttcatt ttgagacact tacatatttt
     1681 aattacatat tttaattttg tctatttaca gaatattata tctacataaa actaaactgc
     1741 tgtaaactta actacttcca gtaatgtttg aagtggtata aaagctagta gcctagtttc
     1801 tacacttata atttatattt tacataacta aatttagtag atttttatac tcacaattta
     1861 gaacgattga aaacttaaaa atttcttatc agatatctac aacttaatta ggta
//
LOCUS       DAGQWR010000274         1886 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.279, whole genome shotgun sequence.
ACCESSION   DAGQWR010000274 DAGQWR010000000
VERSION     DAGQWR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.279"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            76..192
                     /gene="rrf"
                     /locus_tag="MIW95_003867"
     rRNA            76..192
                     /gene="rrf"
                     /locus_tag="MIW95_003867"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            199..273
                     /locus_tag="MIW95_003868"
     tRNA            199..273
                     /locus_tag="MIW95_003868"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:231..233,aa:Asn,seq:gtt)
     gene            278..352
                     /locus_tag="MIW95_003869"
     tRNA            278..352
                     /locus_tag="MIW95_003869"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:311..313,aa:Glu,seq:ttc)
     gene            358..433
                     /locus_tag="MIW95_003870"
     tRNA            358..433
                     /locus_tag="MIW95_003870"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:391..393,aa:Val,seq:tac)
     gene            439..515
                     /locus_tag="MIW95_003871"
     tRNA            439..515
                     /locus_tag="MIW95_003871"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Asp,seq:gtc)
     gene            526..600
                     /locus_tag="MIW95_003872"
     tRNA            526..600
                     /locus_tag="MIW95_003872"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:558..560,aa:Thr,seq:tgt)
     gene            615..699
                     /locus_tag="MIW95_003873"
     tRNA            615..699
                     /locus_tag="MIW95_003873"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:649..651,aa:Tyr,seq:gta)
     gene            709..782
                     /locus_tag="MIW95_003874"
     tRNA            709..782
                     /locus_tag="MIW95_003874"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:741..743,aa:Gly,seq:tcc)
     gene            793..869
                     /locus_tag="MIW95_003875"
     tRNA            793..869
                     /locus_tag="MIW95_003875"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:827..829,aa:Arg,seq:tct)
     gene            879..954
                     /locus_tag="MIW95_003876"
     tRNA            879..954
                     /locus_tag="MIW95_003876"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:912..914,aa:Gln,seq:ttg)
     gene            966..1041
                     /locus_tag="MIW95_003877"
     tRNA            966..1041
                     /locus_tag="MIW95_003877"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:999..1001,aa:Lys,seq:ttt)
     gene            1044..1132
                     /locus_tag="MIW95_003878"
     tRNA            1044..1132
                     /locus_tag="MIW95_003878"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1078..1080,aa:Ser,seq:tga)
     gene            1150..1240
                     /locus_tag="MIW95_003879"
     tRNA            1150..1240
                     /locus_tag="MIW95_003879"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1184..1186,aa:Ser,seq:gct)
     gene            1249..1325
                     /locus_tag="MIW95_003880"
     tRNA            1249..1325
                     /locus_tag="MIW95_003880"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1283..1285,aa:Pro,seq:tgg)
     gene            1411..1486
                     /locus_tag="MIW95_003881"
     tRNA            1411..1486
                     /locus_tag="MIW95_003881"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1444..1446,aa:Trp,seq:cca)
     gene            1547..1623
                     /locus_tag="MIW95_003882"
     tRNA            1547..1623
                     /locus_tag="MIW95_003882"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1581..1583,aa:Pro,seq:tgg)
     gene            1630..1706
                     /locus_tag="MIW95_003883"
     tRNA            1630..1706
                     /locus_tag="MIW95_003883"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1664..1666,aa:Ile,seq:gat)
     gene            1710..1785
                     /locus_tag="MIW95_003884"
     tRNA            1710..1785
                     /locus_tag="MIW95_003884"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1743..1745,aa:Phe,seq:gaa)
     gene            1793..1869
                     /locus_tag="MIW95_003885"
     tRNA            1793..1869
                     /locus_tag="MIW95_003885"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1827..1829,aa:Met,seq:cat)
BASE COUNT          473 a          438 c          517 g          458 t
ORIGIN      
        1 aaaaaaataa aaaaagtatt gacaaaaata gtaaaaaatg ttaatattat acttgtccta
       61 gcaacaagaa aaaaatgtgg ttataatagc agagaagata cacctgtttc cattccgaac
      121 acagaagtta agttctctag cgctgatggt acttggtggg aaactgcctg ggagagtagg
      181 gcatagccac gtgaatgttc caaggtagct caacggtgga gcaaccggct gttaaccggt
      241 aggctgtggg ttcgagcccc acccttggag ccattatggc tcgttggtca agaggttaag
      301 acaccgccct ttcacggcgg taacaggggt tcaattcccc tacgagtcac catttgtggg
      361 atcatagctc agctgggaga gcacctgcct tacaagcagg gggtcacagg ttcgagccct
      421 gttggtccca ccatttgcgg cctggtagtt cagttggtta gaatgccagc ctgtcacgct
      481 ggaggtcgag ggttcgagcc ccttccaggt cgccaaaata aaaatgctgg tgtggctcaa
      541 cggtagagca gctgacttgt aatcagcagg ttgtaggttc gattcctatc accagctcca
      601 tgtaaaacta atacggagga tttcccgagc ggccaaaggg ggcagactgt aaatctgttg
      661 tcatcgactt cggtggttcg aatccaccat cctccaccaa ttaaatatgc gggtgtagct
      721 caatggtaga gttctggcct tccaagccag ctgtgagggt tcgatcccct tcacccgctc
      781 caaagaaagc gtgggtgcat agctcagctg gatagagcaa cgcccttcta aggcgtgtgt
      841 ccggggttcg aatccctgtg cgctcaccac atatatattg gggattagcc aagtcggtaa
      901 ggcacatgac tttgactcat gtatgcgtag gttcgagtcc tgcatcccca gccaaataaa
      961 aatatggtct attagctcag tcggtagagc acctgacttt taatcagggt gtcccgcgtt
     1021 cgagtcgcgg atagatcacc aatggagagg tgtccgagtg gtttaaggag ctggtcttga
     1081 aaaccagtga cttcgaaagg ggccgtgggt tcgaatccca ccctctccgc caaaaaaatg
     1141 actgaatttg gagaagtact caagtggctc aagaggatcc cctgctaagg gattaggctg
     1201 ggtaaccggt gcgagggttc gaatccctcc ttctccgcca ttcaaaaacg aggtgtagcg
     1261 cagtttggta gcgcacatgg tttgggacca tggggccggg ggttcgagtc ccttcacctc
     1321 gaccaactta agcttttagt tcagttggtt agagtgccca actcatggct agtaattcta
     1381 gggtttgagt ccatgaaggt tattcttcat aggggtgtag ctcagttggt agagcgtcgg
     1441 tctccaaaac cgtgcgccgg gggttcgagt ccctccaccc ctgccaaata aaaaggactt
     1501 gaaaaaagtt ggattttaga gtagtataag atagtaaata agatttcgag gtgtagcgca
     1561 gtttggtagc gcacatggtt tgggaccatg gggccggggg ttcgagtccc ttcacctcga
     1621 ccaagaaaag ggcctatagc tcaggtggtt agagcgcacg cctgataagc gtgaggtcgc
     1681 tggttcgagt ccagctaggc ccaccatttg cccagatagc tcagtcggta gagcagggga
     1741 ctgaaaatcc ccgtgtcggt ggttcgattc cgcctctggg caccaggaat acggcggtat
     1801 agcttagttg gctagagcgt tcggttcata cccgaaaggt cataggttcg actcctatta
     1861 ccgctaccat ctaactatat caataa
//
LOCUS       DAGQWR010000275         1852 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.280, whole genome shotgun sequence.
ACCESSION   DAGQWR010000275 DAGQWR010000000
VERSION     DAGQWR010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.280"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            53..1264
                     /locus_tag="MIW95_003886"
     CDS             53..1264
                     /locus_tag="MIW95_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBY3153155.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            1456..1716
                     /locus_tag="MIW95_003887"
     CDS             1456..1716
                     /locus_tag="MIW95_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBY3153156.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
BASE COUNT          779 a          168 c          334 g          571 t
ORIGIN      
        1 tatttgctta agaataaaaa tcagaaacgt ttttgatata gtaggtgata atatgaaagg
       61 attagtttta gaaggtggag gaacaaaagg agcctaccaa ataggtgcat ataaagcttt
      121 aagagagctt ggtatagagt ttcaaggagt aactggcaca tcaattggtg ctttaaatgg
      181 agcatatata attcaaaatg atattgaagt aatggaagat atttggttaa attatgacta
      241 tactcatttt atgaatattg atagagaaac ctatgagagg tttaaaaatg tagatttcac
      301 tactaaaaat ctcaatgcag tgtttgagct tataaataaa gctagaaaaa atgaaggtat
      361 agatataagc ccattaagac aactaatgga gacaacatta aatgaagaca taataaggaa
      421 atcaaaaaaa gactttggac ttgttacggt ctggtgggat aaaaaaatta atccatatcc
      481 attgtatatc gaagatatac ctaaaggaag attgattgat tatttaattg ctagttccag
      541 tttgcctatt tttcaaacga acatgatgga tgataagttg tatcttgatg gaatgttttg
      601 tgataacatg ccaataggac tcttggaaga caaaggttat gaagatgttg ttgtaattag
      661 gttgattgat gattttattg ggaaaatgaa tctaaatcga catcaaaata taaatataaa
      721 aactataata ccatcagaat atctaggagg ttctcttaat aaagatagag ataatgtaga
      781 aaaaaacatc cgactaggtt acttagatac tatgaaagca tatgaaagat gcactggaat
      841 taagtattat tttgatgtag agtataaata taatgaagaa tattgttttg ataaatttag
      901 aagtatgagc aatgaaacaa tacaatatat ctgtaattta ttgaatataa aaagagatgc
      961 ttcacttaga actgttatgg aaagtgtaat accaaagctt ggtgaggtgc ttggattgcc
     1021 aaaggacttt tcatataaag atttgtttta ctgtatatat gaaaagaaac ttgaagaaaa
     1081 taatataaac aggataaaat tatatgattt taacaaggtt atagatgttg taaatacaaa
     1141 tattgattct aatagaaatt tagcttctaa ttttgaagtt agacctaaaa ttagtattaa
     1201 aaagaacaaa aacacaaaat tattgacaaa ttgtattata aaagacataa aaaatcaaag
     1261 ttaaataaaa aacaaaaaaa aatttgtaat tatactcgaa aaaagtgtat attagtagta
     1321 taatattttt gtagcactag tgggaacaaa aaatgaccat aggacaaaaa agtaccactt
     1381 agtaccatta aagtaaaaaa caggaaaaca attttaaaaa atataaaatg taaagtttat
     1441 aggaggattt aaaaaatgga aaaagtagtt agtatcaaaa atgcaagtgg attacatgct
     1501 agaccagcag gaatgtttgt taagaaggca gctgaattta aatcaacagt agaggttgta
     1561 gcaaaagaca aaactgtaaa tgcaaaatca ataatgggaa taatgagttt aggtttagct
     1621 caaggagaag aattaaagtt agttgtaaat ggagaagatg aagaagtagc tttagctgca
     1681 atggttgaat tagttgaaag tggatttgga gaatagatat aataattttt taaaatttga
     1741 tatttagaat ctaatattac ttaaagtaaa gtaacagaga agtattaaac atatacagac
     1801 caacatttgt agatatttaa tttctttgtt actttcatgt ttatttaatc ag
//
LOCUS       DAGQWR010000276         1831 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.281, whole genome shotgun sequence.
ACCESSION   DAGQWR010000276 DAGQWR010000000
VERSION     DAGQWR010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.281"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            172..1602
                     /locus_tag="MIW95_003888"
     CDS             172..1602
                     /locus_tag="MIW95_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBY3153157.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKALKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEDQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEEICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
BASE COUNT          829 a          163 c          320 g          519 t
ORIGIN      
        1 tgtccactat taactttatt accccacata tataagataa aattattaat ataaaatata
       61 atctatcgac aatgaataga cttttaaata aacttaatat aaaattaatt aaataagtgc
      121 ttaaaaacga tttactttaa tgatagaatc atattaaagg aggataattt tatggatata
      181 aagaataaaa ttaaatctat atttttacca gaaaaaaatg actatttaga tgatgaagaa
      241 tttgagtatg atatagaaaa taatgaagaa gatttaaagg aagataacaa agtacattta
      301 aatgatttaa gcagagtaaa atatgattac agagagttaa aagacataga aaaccaaaca
      361 gaagagatgt gtctgctagc agtaaagcag gatggtacta taattgagtt tgtaaaggac
      421 aagacttata aagtatgtat ggaagcagta aaacagacat ataaagctct taagtatata
      481 acaaatcaaa atgaggatat ttgtatagaa gcagtaaaac agaactacag agctttgtac
      541 tacataaata ataagacaga aaatgtacta attgaggcta taaaaaatgc atcaacttat
      601 gatgtcatgg aagtatttaa atttgtggaa gatcaaacag aagatgtatg tctagcattt
      661 atagaaaggg catctaaaaa tgatgttgca gagatattaa aaggaataaa agagcaaact
      721 ccagccatat gtttagaagc tgtaaaaaaa gatggaaaat ctttagctta tgtaaaggaa
      781 caaagtaata gtatatgttt agaagctgta aaagaaaact acagtgcact atcttgtgta
      841 aaagagcaaa cagaagaaat atgtatagaa gcagtaaaac agaatgactt tgcattgtat
      901 tatgtaaatg aacagacaga aaaaatatgt atggaagcag taaaaagaag ttatatggct
      961 ctacagtatg taaataagca aacagaagag atttgtttgg aagcagtaag aattgatgga
     1021 agagcattac agtacgtaaa agagcaaaca gaagaaattt gcttggagtc agttagacaa
     1081 aatggaaaag tattgcaata tgtaaaaaaa cagactgaaa atatatgtat agaagcagta
     1141 agaggttcat ttgaagaact tgaaataaaa gaaatattaa gttatgtaaa gataccaaca
     1201 gaaagaatat ttgtagaagc agtaaagcaa aatggaaaaa ttcttaaata tgtagaaaat
     1261 cagacagaat taatatgttt agaggcagta agagaaaact ataatgcttt agcttatgta
     1321 aaggaacaaa cagaagagat ttgtttagaa gcagttaatc aaagttatga agcgcttaaa
     1381 tatgtgaaag aacaaacaga agaagtttgt ctaaaagcag taaaacaaga ttatagaatg
     1441 ttgaaatatg taaataatca aacagaaaaa atatgtttag aagcagtaaa gcaaaattat
     1501 agagctttag aatttgttga taatcaaaca gaaaaagttt gtcttgaagc agtaaaacag
     1561 aatagaaaag cactacagta tgtaaaacaa aaacaaagtt aaaaggctaa aatatataga
     1621 aaatatatgc aaataaataa aatttgatat tttgtaataa ataaaatggg tatctcaaaa
     1681 tgaacttttt agtttgtgag gtactctttt tatatgaaat aaaatatatt tttagccttt
     1741 taacaatggg gaaaaggttt atttctattt tgacataggc tttttatgca tataaagctt
     1801 gataaaattg ttttattata attgttttgt t
//
LOCUS       DAGQWR010000277         1810 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.282, whole genome shotgun sequence.
ACCESSION   DAGQWR010000277 DAGQWR010000000
VERSION     DAGQWR010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.282"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            191..1240
                     /locus_tag="MIW95_003889"
     CDS             191..1240
                     /locus_tag="MIW95_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY3153158.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWVQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            complement(1390..1737)
                     /locus_tag="MIW95_003890"
     CDS             complement(1390..1737)
                     /locus_tag="MIW95_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBY3153159.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
BASE COUNT          670 a          246 c          281 g          613 t
ORIGIN      
        1 tattacttag tttataaata atcaatataa tttatccaat attacatatt actaaaaaaa
       61 ctaaacataa aactgaaaat tattattaag tttttgtaga aatattataa gcatagatac
      121 atttttgtaa caactacaat aaatttttaa tactatatac tattcgtatg tcttatggga
      181 ggtatttatt atgtttaaaa aaatagtcgc aatagcaagc tctatcattg taagtacaat
      241 atgtctaact ggatgtgatt ttaactctaa tgacaaaaaa gatgaatctt cttcgtctaa
      301 aaatctggat aaagtaacta tagcagaagt tactcactct gttttttatg ctccacagta
      361 tgctgctatt acgaaaggat tctttgaaga tgaaggtata aagattgacc ttataaatac
      421 ccaaggtgca gataaaacta tggcagcttt gatttcaggt gaagcaaacg taggtttaat
      481 gggtcctgaa gcatctatat atgtatataa tcaaaacaaa aacgattatg cagtgaactt
      541 tgctcaattg acaaaaagag atggtagttt tctagttgct aggaaaaaaa tgcctaattt
      601 ctcttacaat gatttaaaag gaacagaaat attaggtggt agaaaaggtg gagttccact
      661 aatgactttt gaatatgttc taaaagaaaa aggtcttact attggagaaa atctaaaagc
      721 aggcaatgtt aatgtaagaa ctgatgtaca atttggtgta atggctggag cttttgcagg
      781 tggagagggt gactttacaa cagcatttga acctacagca acagcaatgg aaaaagaagg
      841 tagtggatat atagttacat ctataggaaa agattctgga gaaatacctt ttacagcata
      901 ctctactacg agatcataca tgaaaaacaa taaagattta attcaaagat ttacaaatgc
      961 tgtttataaa ggtcaattat gggttcaaca agcatctagc aaagaagtag ctaaggctat
     1021 gcaacctttc tttgaagata tatctttaga agatttaaca acagttgtag atagatataa
     1081 atcaatagat gcatggtctc aaaatcctgt attaaaggag gaaggtttga agaaattgat
     1141 gactgttatg aaagaagcta aagaactaga taaggaagca ccttataaag atatcgtaaa
     1201 tactgagttt gcaaacaatg ctataaaaaa tattaaataa aatatattag ttttagctag
     1261 atattttctt gtttttataa tgaaaattaa caaaaatata aaaagagcct tttaaaagac
     1321 tctttttata tttgagatta aagtttatat aataaaatag atattaaagt actaactgta
     1381 cttatatatc tatcctctgt attctgctcc agcattaatt tttgtaattt gaacggctac
     1441 catttcttca ttgcttccat tagtaatttc atgccactct cctgttggta caaatacagt
     1501 atcgccgttt tttacatcac attcatgctc accaagtttc attttacctt ctccttttag
     1561 gaacacaaat atttgttcac cattagggtg tctatgagca ttaagaactt gattaggttt
     1621 aaaataataa acttctgaag ttatatcgtt accagcaaat actggtattt ttggaactgc
     1681 tgtatcatta tattgagcta agcttccgtc taatagattc tttttatttt catgcatact
     1741 agaaatacct cttttctata ttatagattt actattatta taccaatata agaaaatagt
     1801 attctcactt
//
LOCUS       DAGQWR010000278         1805 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.283, whole genome shotgun sequence.
ACCESSION   DAGQWR010000278 DAGQWR010000000
VERSION     DAGQWR010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.283"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            32..763
                     /locus_tag="MIW95_003891"
     CDS             32..763
                     /locus_tag="MIW95_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153160.1"
                     /translation="MLNKKNVSLIVLVIVLSLFTAIFVFNNRNTNDKSKEIAKNNEEI
                     VPKNLEALSIEQLTKIRDKAIDKNLEGLREFGEDSVKQRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQSYEINEFMLKNKKNYKEWSY
                     EYIEFLIDHLDELCKFVDFDIKDVIDIIDPTIKTDLSEEQKKSLNDKLKKVSSSDTLS
                     EDIKKEIKNWENNLNSLNMNKNNSN"
     gene            1005..>1805
                     /locus_tag="MIW95_003892"
     CDS             1005..>1805
                     /locus_tag="MIW95_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBY3153161.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELD
                     DHLGYDRYGNKNKNTKNSRNGYRNKNVKSDFGEITLNIPRDRNGDFEPKVISNYKNDI
                     SGIENQVIGMYSKGMSTRDISSHLKSIYGIDISHSLVAKITDRVLPLAHEWQNRPLDM
                     VYPIVFMDAIHYKVRSEGRIINKAAYIAIGVNLEGIKEVLGIWIGENESSKYWLKVLT
                     DVKNRGVKDILITSVDGLVGFSDAIKAVFPDTEIQRCVVHQIRNTLNYV"
BASE COUNT          771 a          169 c          287 g          578 t
ORIGIN      
        1 aataaaatac ttattaaaaa ggagatttga tatgttaaac aaaaaaaatg taagtttaat
       61 tgttttagta atagttttaa gtttattcac tgctatattt gttttcaata atagaaatac
      121 aaatgataaa tcaaaagaaa ttgctaaaaa taatgaagaa atagtgccta aaaacttaga
      181 agctttaagt atagaacaat taacaaaaat tagagataag gctattgata aaaatttaga
      241 aggtcttaga gaatttggag aagattcagt aaaacagcgt aaagattcaa ttttaaattt
      301 tgaatttgga ttagttgctt ttgttaacaa aaattatagt aacttaacag aaaaagagaa
      361 attaataaga caaattaata ttgttaacta tgaaaactct ataaatgaca taatggatcc
      421 ttcaggttca ttctttgaat tagcaaatca aagttatgaa attaatgagt tcatgttaaa
      481 aaacaaaaaa aattataaag aatggtcata tgaatatata gaattcttaa ttgatcattt
      541 agatgaatta tgcaaatttg ttgattttga tataaaagat gtaatagata ttattgatcc
      601 tacaataaaa acagatcttt cagaagaaca aaagaaatct ttgaatgata aattaaagaa
      661 agtgtcttca agtgatactt taagtgaaga cattaagaag gagataaaaa attgggaaaa
      721 caatttgaat agtttaaata tgaataaaaa taattctaat taactgatag tgtaaaatgt
      781 acataattat aaataaaata gggatgtctt aaaattgatt attttctaat ctaaatattt
      841 taggcctatc taaaaacata agattatggt ctaaaatttg aaattttaga ccatgcttta
      901 tctttacaat aaatgggtct ttaaaataaa gtgtgtattc ttctaaaaag tggtgataat
      961 taaaatatta aatatcacta tataaaggaa ggtgcacact ttttatgttt aacaaaaacg
     1021 aaatattaaa aaaacttatt gaagaatatg atgttaaaac aactacagat attcaagata
     1081 tgctaaaaga tttatttgcg agtacaattc atcaaatgtt agaagcggag cttgacgacc
     1141 atttaggtta tgatagatat ggcaataaga ataaaaatac taaaaactct agaaatggat
     1201 atagaaataa aaatgttaaa tcagattttg gagaaataac tttaaatatt ccaagagacc
     1261 gaaatggaga ttttgaacca aaggtaatta gtaattataa aaatgatata tcaggaatag
     1321 agaaccaagt gattggaatg tactcaaaag gcatgtctac tcgagatata tcaagccacc
     1381 ttaagagtat ttacggaatt gatatatctc attctctagt agctaaaatt actgatagag
     1441 ttctaccttt agctcatgaa tggcaaaaca gacctcttga tatggtttat ccaattgtat
     1501 ttatggatgc tatacattat aaagttagaa gtgaaggtag aataataaac aaagctgcat
     1561 atatagccat aggtgttaac ttagaaggta taaaggaggt cttaggaata tggattggag
     1621 aaaatgaaag ttcaaagtat tggctaaagg tgcttacaga tgtcaaaaac agaggagtta
     1681 aggatatatt aataacttct gtagatggat tagttggatt ttctgatgca attaaagcag
     1741 tatttcctga tactgaaata caaagatgtg ttgtacatca aattagaaat actttaaatt
     1801 atgta
//
LOCUS       DAGQWR010000279         1797 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.284, whole genome shotgun sequence.
ACCESSION   DAGQWR010000279 DAGQWR010000000
VERSION     DAGQWR010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.284"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            220..>1797
                     /locus_tag="MIW95_003893"
     CDS             220..>1797
                     /locus_tag="MIW95_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBY3153162.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
BASE COUNT          705 a          146 c          352 g          594 t
ORIGIN      
        1 taattagtgt aaataggttt taaatcaaca taataactta aattatatgg tataataaat
       61 taaataattg ttttaattca aaattttttg ttattttaaa ttatttcaaa actcttagtt
      121 ttagtgattt tatttatgat atttatggga ttaaaagatt tcaatagata tttttggtaa
      181 gtatttaaga agtctttaac tttataggag tgataattta tgctaatagg tacatctaaa
      241 ttaagcagaa atgttgatac tgcatgtgta gaagaagtag ggattccaat gattgtattg
      301 atggaaaatg cggttatatc tgcaatgaaa aatatggata tagacatgta taatagttat
      361 acagttgtat gtggtgttgg aaataatgga ggagatggtc ttggaatagc aagacatcta
      421 aatacatatg gaaaagaagt aaacgtattt ttagtaggta atcttgaaaa actaagtgaa
      481 tgctctaaaa taaattacaa tgtactttta aatatgggaa ttaatattat aaatattaat
      541 aaaaatgatt taagtgaaaa aaattatgga gatgaaaatg aagtagataa aattattaaa
      601 gttgagaaac aaaatattat aaaaaataaa aatttaacaa tttttaaaga tagagtatct
      661 aaaagtgatg ttgtagtaga tgctatattt gggactggat taaaacgtga tataactggt
      721 atttttaaag aagttattga catagtaaac gataatagca aaaatacata ttctatagat
      781 attccttcag gtatagatag cgataatgga aatgtattag gaacttgtat tagagcaaac
      841 aaaacgatta gctttgagtt ttataaaaga ggttttctaa actatgacac tagcacactt
      901 ataggtgatg tagttgtaga aaagataggt gttccagatt ttattacaag aaaataccat
      961 gatgaagagt tcataactga taagactttt gtaaaaaata atattagaag aagagataag
     1021 catggtttta aaagtgattt tggaaaagta tctatagttg caggttcaaa aggattttat
     1081 ggtgcaagtt tcatagcaac tgaatctgct gttaagagtg ggagtggact tgtaacatta
     1141 attagtagtg aagatgtgca agaaaaggta tctattagac ttacagaagc aatgacttta
     1201 aattttgaag aagaaagatt aaataaatta cttaattcta gtgatgcaat tggttttggt
     1261 ccaggtatgg gagataatag tcaaacattt gataaattac ttaagatagt agaaaattct
     1321 aattgcccaa tagtattaga tgcagatgga ttgaacgtta tgaaggacag atgttataag
     1381 tttttagaat ggaaaaatag atttgtaatt acacctcatt tgggagaaat ggccaggttg
     1441 acaggagatt caattgggta tataagagaa catagagtag atgttgcaaa agaatttgct
     1501 caaaaatata atctggtggt gcttttaaaa ggttatcaaa ctgtcataac agatggtaaa
     1561 aaaacatata taaatcctac aggaaatagc tgtatggcaa ctggtggaat gggtgattgt
     1621 ttgcttggaa tgattacttc atttataggt caaggtatgg atatattgga agctacagtt
     1681 agtggagcat atatacatgg ctatatagga gataaattga gcaaagcaat gtatacggta
     1741 aatgctacgg atttaataag taacatttca ttaacaatga aagagttgtt ggatata
//
LOCUS       DAGQWR010000280         1768 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.285, whole genome shotgun sequence.
ACCESSION   DAGQWR010000280 DAGQWR010000000
VERSION     DAGQWR010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.285"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            42..1736
                     /locus_tag="MIW95_003894"
     CDS             42..1736
                     /locus_tag="MIW95_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBY3153163.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSHFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGNIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKISKKLGFEDFVEL
                     GYVRMMRSDYREDMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
BASE COUNT          714 a          154 c          317 g          583 t
ORIGIN      
        1 ttataaatac aataaagtaa agttgtaaag gagtgcttta aatgaagttt tcagagttta
       61 agtatgaaag acccaattat aatagtatga aaaaagagtt tttgagttgt gtagaagata
      121 taaataattc tcgtaattat aaagagcaac aaaaaaatat acataagatt aatttattaa
      181 gaaataaaat agaaacgttg tcaaatatag cttcgataag atatagtaca gacacattta
      241 ataaattcta caaagaagaa aaaaattatt gggatgaata tatgcctttg tatgaagaat
      301 taaattcaca tttttataat gctattgtaa attcaaaatt taaatatgat ttgataaaag
      361 agtttggaga acaatttttc acaatagtag agtattcttt aaaaagtttt tctaaagaaa
      421 taataagtga attgcaggaa gaaaataaat tgtgttcaga gtatactaga cttttagctt
      481 cagctgagat aatgtttgat gggaatataa gaaatttatc tggtatgggg aaattcatgt
      541 attctaagaa tagaaaaaca agagaattag caaacaaagc atattataat ttctttgaag
      601 aaaatgaaac taaatttgat gacatatttg ataaattggt taaacttaga gataagatat
      661 ctaaaaaact aggttttgaa gattttgtag agcttgggta cgttagaatg atgagaagtg
      721 actacagaga agatatgata aaaaatgtta gaaaacaagt attaaaatat gttgttccaa
      781 tggccaatga gctttatgaa aaacaagcta aaagaattgg tctagaatat ttaagttaca
      841 tagatgaagg cgtggaattt ttaacaggaa atgcaagttt aaagggagat tcaagataca
      901 taattcaaaa tggtaaaagg atgtattctg aactgtcaaa agaaacaaat gaattttttg
      961 attttatgtt agaaaatgaa cttatggatt tagaaacgaa aaaaggaaaa ggagctggag
     1021 ggtattgtac ttatatacca gattataagt caccattcat tttttctaat tttaatcaaa
     1081 ctgctgatga tatagatgtg ttaacacatg aggcaggaca tgcatttcag ttgtatatgt
     1141 ctagatggat tgatatgcca gaaattaatt ttccaacgct tgatagttgt gaaatccatt
     1201 caatgagtat ggaatttata acatggccgt ggatggattt attttttaaa gaagatacag
     1261 ataagtacaa attcactcac ttatcatctt caattaaatt tataccttat ggagtaattg
     1321 ttgatgaatt tcaacactat atttacaaaa atcctaatgt agataagtca aaacgaaaag
     1381 aaatctggag atttttagag aaaaaatact tacctcatag aaaatatggt gataattctt
     1441 ttttagagag aggttgttgg tggtttaaac aaggacatat atttaaaaat ccgttttatt
     1501 atatagatta tgtattagct caaatttgtg cgttacaatt ttggaaaaaa atgattcaag
     1561 atagagatgc aggatggaaa gattatataa atatctgtaa agttggagga acaaaatcat
     1621 ttttagatat tgtgagtatg ggaaatttgt attctccatt tgatgatggc tgtattgagt
     1681 ctattatagg tgatgttaaa tcttggtttg atgaaataaa tgacagcaaa ttataaaaaa
     1741 ttatgagaat tttattgtat aaaagtga
//
LOCUS       DAGQWR010000281         1763 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.286, whole genome shotgun sequence.
ACCESSION   DAGQWR010000281 DAGQWR010000000
VERSION     DAGQWR010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.286"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            164..352
                     /locus_tag="MIW95_003895"
     CDS             164..352
                     /locus_tag="MIW95_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153164.1"
                     /translation="MEIKKNTQDVVSERKNSLDSEFKIPASGVCYMAEFIKESREIIK
                     ELDKHFESCLDVLSKARL"
     gene            complement(332..649)
                     /locus_tag="MIW95_003896"
     CDS             complement(332..649)
                     /locus_tag="MIW95_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153165.1"
                     /translation="MSKNIDIRNLKQFQKKIERMEQNLKPDFDKIRTIILSKKFMNQH
                     TNFDSFDELLAFGNYIVNSEEDFLAIPDNEFDLFIVKNTDFPDWQTMLDSAYSKYLES
                     CLR"
     gene            773..1000
                     /locus_tag="MIW95_003897"
     CDS             773..1000
                     /locus_tag="MIW95_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153166.1"
                     /translation="MGFEISGNLNFDSVIDDLKKEVENNPTIFTSQNVGNKFKEKCKI
                     CEKISEFEILEDGKVKCLECGTEFELNLKVE"
     gene            1070..1762
                     /locus_tag="MIW95_003898"
     CDS             1070..1762
                     /locus_tag="MIW95_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3153167.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
BASE COUNT          722 a          183 c          330 g          528 t
ORIGIN      
        1 tttggaaaaa gaagccttga taaagacgaa acagattcgt tagatgggga ctatttagtt
       61 caaatgttga aatatctaaa aaaataaaag tacataaaat ctaaaatgac tatcttgata
      121 gatggtcttt ttttatacaa taaattaaaa ggagaatgaa attatggaga ttaagaaaaa
      181 cacacaagat gtagtatcag aaagaaaaaa ttccctagat tctgaattca agatacctgc
      241 aagtggtgtg tgttatatgg ctgagtttat aaaggaatct agggaaatca taaaagaatt
      301 agataaacat tttgaaagtt gtctagatgt tttatctaag gcaagactct aaatattttg
      361 aatatgctga atcaagcatg gtttgccaat ctgggaaatc agtattttta actataaata
      421 aatcaaattc attatcagga atagctaaaa agtcttcttc tgaattgact atgtaattac
      481 caaatgcaag tagttcatca aaagaatcaa aattagtgtg ttggttcata aatttcttgc
      541 taagaattat tgtgcgaatc ttatcaaagt caggttttaa attttgttcc attctttcaa
      601 ttttcttttg aaattgcttt aaatttctaa tgtcaatatt tttactcata agaacacctc
      661 ctttcaatag aatattagca taaaattgtg gtgaattctg ctattgtcga acgattgttg
      721 aaggatattg tataataaca tagaatttac tatactataa ggaggtgatt atgtgggatt
      781 tgagataagt ggtaatttga attttgacag tgttattgat gatttaaaaa aagaagttga
      841 aaataaccct actatattta catcacaaaa tgttggaaat aagttcaaag aaaaatgtaa
      901 aatatgcgaa aaaatatctg aatttgaaat actagaagat ggtaaagtta aatgtttaga
      961 atgtgggact gaatttgaat tgaatcttaa agtagagtaa acaaaaaaag aatctcaatt
     1021 atgaggttct ttttttattc ccaaaacaaa caaataaaga ggtggtgatg tgcaagatgt
     1081 caaagaaaag gtaaaacaag attacttaaa aggaatgaaa caaaaggaaa tatcatcaaa
     1141 gtatgacatt agcttaaaca ctttaaagtc atggataaaa agatacaact gggctagtga
     1201 aaaaaagaag ggtgcaccta taaataaaag aggtgcaccc ttttctaata aaaattcagt
     1261 tggtcatggt gctccaaaag agaataagaa cgctgaaaag tttggtttct tctcaaaata
     1321 tctacccgaa gaaactaggg aattgataca agaaatatcc ataaaagata aatttgatat
     1381 tctttgggag cagataacaa tccaatacgc agcaataata agagcacaaa agataatgta
     1441 tgttaaagac aaggaagaaa tgattaaaga attaaagaaa catgaaagta cagaaaatgg
     1501 agagaagata gagtatgaat ttcaatttgc atgggatagg caagcatctt ttcttaatgc
     1561 acagagtaga gctatgagtg aacttagaag tttaattaaa cagtatgatg aaatgattca
     1621 taaggattgg aatttggcta cagaggagca gaaaacaaga gttgagaagt tgaaatgtga
     1681 agttgataac ctaagtaaag atgatattgg agatgatgag ttgaaaataa gtgtagatta
     1741 tggtgataga aatgatagtt aga
//
LOCUS       DAGQWR010000282         1758 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.287, whole genome shotgun sequence.
ACCESSION   DAGQWR010000282 DAGQWR010000000
VERSION     DAGQWR010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.287"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            283..738
                     /gene="smpB"
                     /locus_tag="MIW95_003899"
     CDS             283..738
                     /gene="smpB"
                     /locus_tag="MIW95_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBY3153168.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            896..1245
                     /gene="ssrA"
                     /locus_tag="MIW95_003900"
     tmRNA           896..1245
                     /gene="ssrA"
                     /locus_tag="MIW95_003900"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          667 a          202 c          311 g          578 t
ORIGIN      
        1 atttataata tgtaatattt ttgttttgaa atttagaaat cagtgttact ggttaaagtt
       61 agtttagtat taagtatatt cgaagtagtt ttttagctat tttcatatct ttatattact
      121 taattatata tagcctaact aacttaatat atcagtgaaa tattgactaa aatgcctaaa
      181 tatgttatca tacaagttag tcaaataatt tggcaacatt aaatatttag taaatttcta
      241 acatttatgg aataaatttc taacaaagaa ggtgagattt atatggctgg taaaaaaata
      301 ttagcaacaa acaaaagagc aagacatgaa tattttatag aagaaactta tgaatgtgga
      361 attgaattaa aaggaacaga agtaaaatca ataagacaag gcaaagttaa cctaagtgat
      421 ggatttgcat ctgtagataa tagtgaagta tttttaaaac aagttcatat aagtccatat
      481 gagcaaggaa atatattcaa tgttgattct ttaagagtta gaaaactatt acttcataag
      541 catgaaataa gaaaacttat tggagcaact actataaagg ggtattcact tataccactt
      601 agtttgtatc taaaaaacgg taaagttaaa gtagagattg ccttagcaaa aggtaaaaaa
      661 ctatatgata aacgtcaaga tatggctaag aaagatgcac aaagacgtat agaaagagaa
      721 atgacaggta agtattaaac tagataaaac tgtatttttt agaataaaat tctttattat
      781 ctagaacata acattgattt ttattcttaa aagtattata ttatatatac attaaaaatt
      841 taataaaaat ggtgataaag tttgcgaaaa gtctactgga ctataaacta ctcaaggggg
      901 cgtaaaggtt tcgacgtggg tttggaactt gaggctgcat gtcgtgttac tctgggtcac
      961 gtaaaaactg gggaacttta aaataaacgc agacgataat ttcgcgatag cagcctagtt
     1021 gctgctcgtc cctcttagtc ctcctgccga ctaagactgg acgtcattat gcagggaact
     1081 actttagggg tgtctcgacc tgaagtagat taattgggac tggctaagca tagagcctgc
     1141 cacttggcgc tatgtgaggt gagatattag tgaagttggc taagcatgta gatgcagcaa
     1201 ggaaaagatt tgcggacagg ggttcgactc ccctcgtctc caccaatttt taaaaaagaa
     1261 tgatgaaata cttggaaata ctagtcttta ttgactagtg ttttttgttt tggagatgaa
     1321 atccaccaac cgtgccccaa ccacagaaaa agagattttt ttggggtata ttggggtgca
     1381 tttgattaat ccatggaaca tataaagaat acttttagag atatagtgaa tttgatgata
     1441 atacaataaa tattttatac tgatgaattg tttttatatt atgcttttta tttcaatcta
     1501 ggaaatatat aacttgttta aacttatgat taaaaggata aagaagttta aagcatataa
     1561 gtgctactta atctattaga gtaattacta gttataatga tatacagaat cctataagat
     1621 tatgtataag tatagaacaa atttattatc tatgtgggat gctaatttaa attttttaat
     1681 tgtatttaaa aaaataagat gtaaaaaagg aattgaaaaa tgttaagata ataaacacat
     1741 agaattatta agtttaat
//
LOCUS       DAGQWR010000283         1748 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.288, whole genome shotgun sequence.
ACCESSION   DAGQWR010000283 DAGQWR010000000
VERSION     DAGQWR010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.288"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            301..1593
                     /gene="brnQ"
                     /locus_tag="MIW95_003901"
     CDS             301..1593
                     /gene="brnQ"
                     /locus_tag="MIW95_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBY3153169.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIKFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          630 a          212 c          310 g          596 t
ORIGIN      
        1 aataaaacga ataatctaaa aagtcttaat attttaattg ttctgaatag ttaattttat
       61 caaggaaaat tagagaaaaa acaaaaaacc aactatgttt aaagtataca ggttgatttt
      121 gtttaattaa gaaagttgta ttaaatattg tgaagatttt ataaattatg aaacataatt
      181 gacaaattta agtcaaaaat gtataattaa ataaaatctc gtcaaaaggt tttcatttaa
      241 tgaagagatt ggacttattt tttagtaaaa ggtagtaaaa ttcaaatggg ggtgctttaa
      301 atgggtgaga aacaaatcaa ttttaaagat gtaattgtaa ttggatttgc actatttgca
      361 atgttttttg gtgcagggaa cttaatcttc cctccatatc taggagtttt atctggaagt
      421 agttggctaa tagctttcat aggattcctt tttgcagatg gtggtttggc attattagca
      481 gttatagcag caactaagtt caatggagac acatcgaaga tgttctcaag agcaggcaaa
      541 ggattaagta ttgcattagg atgtgcaatg gtaatctgta tagggccact tttagcaata
      601 cctagaacag cagcaacaac ttatgaaatg ggtatattgc ctacaatagg ttctggaata
      661 agtccagtaa tattctctat agtattcttt gctctagtat tggttttaac aataagacca
      721 tcaaaagttg tagatatagt tgggtcaata ttaactccag ctttacttat agctttagca
      781 gtattaatag ttaaaggtat cgtatctcca ttaggagaaa taagagacag ttctttaatt
      841 caaaatgtat ttgcagaagg tataactcaa ggttatcaaa caatggatgc attggcagct
      901 agtgtatttg catcaattat aatcatgtca gtaatagcta aaggttatac aggtgagaaa
      961 gaaaagatga aagccacaat aagtgcaggt gtaattgcag taataggaat ggcattagtt
     1021 tatggtggat tatgttatct tggagcaact gtgtctcaac aatatggaca agatgttcaa
     1081 caaacagctt taatagttgc aataacagca gctttacttg gaactacagg taaaatttta
     1141 ttggctatta tagttgcatt agcatgttta acaacagcta taggtctttc ttcagcagct
     1201 ggtcaatatt tctcaacact tacagatgga aaattaaagt atgagcatat agttatagta
     1261 gtttgtgtat ttagtgcaat catatcaaac tttggagtaa gtacaataat aaaattctct
     1321 tctcctatac taagtatggt atatccagca actataactc tagttatact agcattattt
     1381 agtaataaaa ttaaaaatga caacgtgttc aaatgtgctg catacatggc attattagta
     1441 agtgtattaa cagttgcaac aagttttggt gtaaaaattc ctctagttaa tagcttacca
     1501 tttgcttcat taggatttaa ctggatagtt ccagttgtaa tagcaggaat aataggaaac
     1561 tttattccat ctaaaagcag taaaactgca taataataaa actaattgat aattgtggaa
     1621 ttgcctttaa attatatggc aattcctttt tatatttaaa agaattatta aattattgtg
     1681 gagaaaagaa cattattaat ctcatttgat aaatttattt ttggaggagt atgatttata
     1741 aagacaat
//
LOCUS       DAGQWR010000284         1731 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.289, whole genome shotgun sequence.
ACCESSION   DAGQWR010000284 DAGQWR010000000
VERSION     DAGQWR010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.289"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            205..1458
                     /locus_tag="MIW95_003902"
     CDS             205..1458
                     /locus_tag="MIW95_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBY3153170.1"
                     /translation="MLNKKNISSIIMTMILSLSTTVFVFADENTNVKGQDVTKYNEEM
                     VSKNLEDLSIEQLIDIRDKAIDKNLEGLRKFGEDSVKERKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVEYENSINDIMDPSGDFFKVTNQSYQINEFVLKNGKNYKEWSY
                     EYVEFLIEHLEDLCNFVDFDAKYVIEMVDPSIKPDLSDNQKKSLNNELIKMYSSNTLS
                     TNIKDEIENWENNLNINEKNSNEPLPRVSPSSYRQQALAYAKKHGYTNGYYGSTNRKN
                     NAPSPYYNFQKDGYGDCANFVSQCLHEAGVTFWTNNNPWYYYSTSNRSPSWAGAREFK
                     IHWMNRIAYQTLTVSNSLSFLRPATPVSILNSSGVATHTLISTDKHSNGYNFSYAAHS
                     DEGARTNLLKKLEGKKISYYKVYWE"
BASE COUNT          727 a          169 c          254 g          581 t
ORIGIN      
        1 ataaaaaaat ttatatataa gtgtctaaaa aacgattctt tattcgttat atatataaat
       61 aatactagaa agggaggatt atattatgat atttagtaaa cgctctttta gaaaaaaacg
      121 gtgataaact tgttttaaat tttagtagaa tatgattttt gttttggcaa gtattaatac
      181 atatattaaa gagggaggtt taatatgtta aataaaaaaa atataagttc aattattatg
      241 acaatgattt taagcttatc aactactgta tttgttttcg ctgatgaaaa tactaatgtg
      301 aaaggacaag atgtaaccaa atataatgaa gaaatggtct ctaaaaactt agaagattta
      361 agtatagaac aattaataga tattagagat aaagctattg ataaaaattt agaaggtctt
      421 agaaaatttg gagaagattc agtgaaagaa cgtaaagatt caattttaaa ctttgaattt
      481 ggattagtag cttttgttaa caaaaattat agtaacttaa cagaaaaaga gaaattaata
      541 agacaaatca atattgttga gtatgaaaat tctataaatg atataatgga tccttcaggc
      601 gattttttca aagtaacaaa ccaaagttat cagattaatg aatttgtatt aaaaaatgga
      661 aaaaactata aagagtggtc gtatgaatat gtagagtttt tgattgagca tttagaggat
      721 ttatgtaatt ttgtagattt tgatgcaaaa tatgtaatag aaatggttga tccttcaata
      781 aaaccagatc tttcagataa tcaaaagaaa tctctaaata atgaattaat aaaaatgtat
      841 tcaagtaata ctctaagtac aaatataaaa gatgaaatag aaaactggga aaataattta
      901 aatattaatg agaaaaattc aaatgaacct ttaccaagag taagcccttc tagttataga
      961 caacaagcat tagcttatgc taaaaaacat ggctatacta atggttatta tggtagtaca
     1021 aatagaaaaa ataatgcacc ttctccatat tataattttc aaaaagatgg atatggagat
     1081 tgtgctaact ttgtttcaca atgcttacat gaagctggag taacattctg gacgaataat
     1141 aatccatggt attattattc tactagtaat agatcacctt cttgggctgg agcaagagaa
     1201 tttaaaatac attggatgaa tcgaattgca tatcaaacac ttactgtatc aaactcttta
     1261 agttttttaa gaccagcaac acctgtaagt atattaaata gtagtggagt agcaactcat
     1321 acactaatat ctacagataa gcattcaaat ggatataatt tttcatatgc tgcacattca
     1381 gatgaaggtg caagaacaaa tttgttaaaa aaacttgaag gaaagaaaat ttcttattac
     1441 aaagtttatt gggaataaat aaaaatttat acttttaatt aaatgaaaat tgaaaagaat
     1501 aaatatctat ttcaagatcc aataagtatt tagttgaagc agctattaaa ttttattaca
     1561 taatcataaa aaagagacta gtggaattta gtctcttttt tatgatttaa aaatttaata
     1621 aaagaattat ataaatttgt ctaaaaatta gtttatcatt cgttataatt atataaacta
     1681 tatcaaaaaa gtgttctatt gtattcttta gaataatcag taataaaggt t
//
LOCUS       DAGQWR010000285         1705 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.290, whole genome shotgun sequence.
ACCESSION   DAGQWR010000285 DAGQWR010000000
VERSION     DAGQWR010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.290"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            188..1000
                     /locus_tag="MIW95_003903"
     CDS             188..1000
                     /locus_tag="MIW95_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBY3153171.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1089..1280)
                     /locus_tag="MIW95_003904"
     CDS             complement(1089..1280)
                     /locus_tag="MIW95_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153172.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
BASE COUNT          634 a          196 c          238 g          637 t
ORIGIN      
        1 gaaataatca ctttttcaag aatgttataa tttttttatt gtattctttt tgctatagtc
       61 tatgaataaa tcaatagaaa atcttctaag ttgcttttaa ataatgactt tatggttcaa
      121 ttgtactaaa atagtatatg taggttacaa ataatctaat aaatattaaa gtttggaggt
      181 aatagatatg gcagattgta attcatgtcc atcaaaggga aattgtaata gtcaatcaaa
      241 ttgttctatt gaaaataacc ctaacaataa atttggaaaa ataattggtg ttatgagtgg
      301 gaagggtgga gttggaaaat caactgtaac tgctttactt gctaataagc taaacaaaat
      361 gggatataag gttggtatat tagattcaga tataactgga ccaagtattc ctagacttat
      421 gggtgtaaaa aatgtaaaag cttattctga tggctcttat atttaccctg tagaaaactc
      481 aaataacata aaagtaatgt ctataaacct tatgatagat gatgagaatg aacctgttgt
      541 ttggagagga cctttacttg gaggagttgt aaagcaattt tatactgatg tactttggga
      601 agaattagac tatttattaa tagatatgcc tcctggtact ggagatgttg ccttaacggt
      661 tatgcaatct attcctataa gtggaatagt tatggtttca gtacctcaag atttggtatc
      721 tatgattgta tcaaaagctg ttaatatggc taaaaaaatg aacataaatg tccttggagt
      781 tattgaaaat atgagttata tccaatgtcc tgattgttct aaaaaaatca agttatttga
      841 aggagaatct actgaaaagt ttttagatga tttagactta gagcttttag gagaacttcc
      901 tatgactaaa gaaattatag atattactca taatggagtt acagaaatta gtgatgattt
      961 agattcaata ttgactaatg ttgttgaaaa aattaaataa ataaaaattt tataatttta
     1021 tatttatcaa aaaaagaggc ttgtatttat tttataatca tacaaataaa tataaacctc
     1081 ttttatgttt atctatgttt taaatacaca aaatattctt ctacttcatc tccatttttt
     1141 acttttacta agtattcatt ataatgagca ttatatggct gtgtcatttt gcattctatt
     1201 acattttcta tgtcttctaa ttctaaaagt tctacatcct ttaaaaattt tctttctatg
     1261 agttccatta atctttccat aatatatcct cacttcccct ataattaaga taaactatat
     1321 atcctcttaa tacagagttt atctgtttat tatattatat ccaatatatt acagtttcta
     1381 acatcattca taaattattt taacaaatag agattgattt tttaataaat atgccttgat
     1441 aaaatgaaac atagaccaac tcttagtttt atgtaaagaa aacttaaaga aaactataga
     1501 ttatattcac aaaaactact cagaagatat aacagtaagt aatatatgta atgaattaca
     1561 acttaataaa agttattttt ttctttgttt aagaaaaaaa gtggatatac ttttactaat
     1621 tttttaaata aagttagagt tgaaaaaaac aaaaagtatc tattaaaaaa agacttgtct
     1681 attttagacg ttactgtact aattg
//
LOCUS       DAGQWR010000286         1662 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.291, whole genome shotgun sequence.
ACCESSION   DAGQWR010000286 DAGQWR010000000
VERSION     DAGQWR010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.291"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..>1662
                     /locus_tag="MIW95_003905"
     rRNA            <1..>1662
                     /locus_tag="MIW95_003905"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          477 a          347 c          492 g          346 t
ORIGIN      
        1 gggacacgtg aaaccctgtg ggaagcagga gggaccaccc tccaaggcta aatactacct
       61 agtgaccgat agcgtatagt accgtgaggg aaaggtgaaa agaaccccgg gaggggagtg
      121 aaatagaacc tgaaaccctg cacttacaag ctgtggaagc acatttcttg tgtgaccgcg
      181 tactttttgt agaacgggcc aacgagttac gttaagtagc aaggttaagc acttaaggtg
      241 cggagccgta gcgaaagcga gttttaactg agcgttcagt tacttgacgt agacccgaaa
      301 ccgggcgacc tacccatgag caggatgaag cgaaagtaaa atttcgtgga ggtccgaacc
      361 cacgagcgtt gaaaagctcg gggatgactt gtgggtagcg gtgaaattcc aatcgagccc
      421 ggagatagct ggttctcccc gaaatagctt tagggctagc ctcaaggtga gagatacgga
      481 ggtagagcac tgaatgtcct agggggtatt gcacctaccg aagactatca aactccgaat
      541 gccgtcatct tatacttggg agtcagactg tgggtgataa gattcatagt cgaaagggca
      601 acagcccaga tcgtcagcta aggtccctaa atgtaagtta agtggtaaag gatgtgggat
      661 tgcacagaca accaggatgt tggcttagaa gcagccactc attcaaagag tgcgtaatag
      721 ctcactggtc gagtgatcct gcgccgaaga tttccggggc taaaacttac taccgaagct
      781 acggcatcag taatgatggg taggggagct tcccatacgg gttgaagcat gaccgtaagg
      841 acatgtggac agtatgggag tgagaatgtt ggcatgagta gcgagatgtg ggtgagaatc
      901 ccacaggccg taaacccaag gtttccaggg gaaggttcgt ccgccctggg ttagtcggga
      961 cctaagctga ggccgaaagg cgtaggtgat ggacaacagg ttgatattcc tgtactaccg
     1021 ataaccgttt gagagaaggg atgacacagt aggataagct aagcacactg ttggttatgt
     1081 gtgcccaagc attgaggcag tcaaagtagg caaatccgct ttgataatgc tgggatgtga
     1141 tggggagcga aatttagtag cgaagtagct gatttcacac tgtcaagaaa agtctctatc
     1201 gaggttaaag gtacccgtac cgcaaaccga cacaggtggg tgaggagagt atcctaaggc
     1261 cagcgagaga actgttgtta aggaactcgg caaaatgacc ccgtaactta gggataaggg
     1321 gtgccaccat caggtggccg cagagaatag gcccaagcga ctgtttacca aaaacatagg
     1381 tttctgctaa gtcgcaagac gatgtatagg agctgacgcc tgcccggtgc tggaaggtta
     1441 aggggatctg ttagagcaat cgaagcagtg aacttaagcc ccagtaaacg gcggccgtaa
     1501 ctataacggt cctaaggtag cgaaattcct tgtcgggtaa gttccgaccc gcacgaaagg
     1561 cgtaacgatt tgggcactgt ctcaacaaca gactcggtga aattgtaatt ccggtgaaga
     1621 tgccggatac ctgcgacagg acggaaagac cccatggagc tt
//
LOCUS       DAGQWR010000287         1657 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.292, whole genome shotgun sequence.
ACCESSION   DAGQWR010000287 DAGQWR010000000
VERSION     DAGQWR010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.292"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(181..1341)
                     /locus_tag="MIW95_003906"
     CDS             complement(181..1341)
                     /locus_tag="MIW95_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBY3153173.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALVVTQILHMLA"
BASE COUNT          619 a          292 c          173 g          573 t
ORIGIN      
        1 tataaaaatt aaatatatct cgtttttagt aaattttaca atattataca ctaaatatgt
       61 caaaacatct tcttgaaata gattatcttg tcaaacaaaa aaaaccattt cagtaaaagc
      121 ttctgcttaa attgaaatga cttttcttat ttaaattatt aattttatac catattttta
      181 ttaagctaac atatgtaata tttgagttac cacaagagca caataagtcc ctataatatt
      241 tcctagaagc cccataagta cacctactgg tattaataca ggattgtgaa ccccagcaat
      301 cattggagct gtagttactc ctcctatatt agctatactt cctacttcac aagtaaataa
      361 atcaagttta aatagtttag caagccctat aaatgaaata gcatgtacaa ggactactac
      421 caatccaaac attatgtaaa caggtgcttc tgacaatcca aagaaatttg ctttactacc
      481 tgtagctgca aggagtaaat acataaacac atttcctacc agtgtactcc ctttaagctt
      541 tgatattttt gtcataccca ataatattga tactgtagat gcaacaagta ctacccacat
      601 agtttcatta agtatacctg aagttggaag tatcttggat acaatttgtg caaaagacgc
      661 aactccaaac ccaacagaaa gtaaaatcat aagttctaca aacgtaggtg ccgtattatc
      721 tttattttct ttttcaagtt ttgatgtaac ctcatctata aaacttgtat cgcactttgt
      781 ccatttgtta aatttaggtc caaatttcac agacattaga caaacagaca tccatactgt
      841 atatcctata gaacccataa gtattgcata actaagtcct tcttctgcaa ctcctagagc
      901 attagctgct gcaatcatat ttgatgagcc acctatccat gaaccagatg ctgccccaag
      961 aattttccat gaatctgcac ccattgaatt tttaaaaatt atgaatgcta taacaaatcc
     1021 aagcataaat gtaaatgtag cacagaaaaa tgataataac attttaggcc ccatctttaa
     1081 tatttttctt aaatcacatt gaattaacaa gaacactaac attaatggaa ggaagttcac
     1141 tgttattatg ttttgatacg ttccaacatc ttcattctgt gcaaataaac caaatgatcc
     1201 aagtatagct gaacctaagt atattaaaag cattggatta ataacatcaa ataccttcca
     1261 tttcaattta tcaaccataa aaaaaattac acctacgaat acaaccaaga aactacaata
     1321 tacaaatcca gtctcaatca cattatacac accctttctt ttcctaatta atttgatttt
     1381 tatgatttct aagtaaagac tttttcagat aatagacaat taaaataaat acttttatct
     1441 atatcatgct aattaaatac atatatacct aactattgac atatattaat aaagatttgc
     1501 acattaatta ctttagaaat gaataatctg aatttaaaat ctttttcaac aattatttta
     1561 ttactaaaat ttttatttgt aaagataatt aaactaaata ttttattatt tatttatttt
     1621 gttttgtatt tatttttatt aattattaaa taaaact
//
LOCUS       DAGQWR010000288         1651 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.293, whole genome shotgun sequence.
ACCESSION   DAGQWR010000288 DAGQWR010000000
VERSION     DAGQWR010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.293"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            289..1647
                     /locus_tag="MIW95_003907"
     CDS             289..1647
                     /locus_tag="MIW95_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBY3153174.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     ASGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
BASE COUNT          558 a          192 c          302 g          599 t
ORIGIN      
        1 tataatgatt aagattgaaa gatttaaatt gaattaaatt tactataaaa aattaaaaat
       61 tcatagctat aatactctta tatttgtaaa gcatggtata taatgtatac aaaattaaag
      121 aatacgtgta tttgttaata attaaattta ttgcatctgt tttttaagac taaaaactta
      181 aatatatgtt ggagtatagt tgatatttat gaacatcatt aatttaatat attagatttt
      241 taagtattta tataattcaa taagttttat gtaacgggag tttttattat gaaagcaaaa
      301 agtacatcaa aatttgaatt agacggaata ccaccactaa aggaagcaat acctttaggg
      361 ttacaacact taatggcaat ggtaataggt agttgtttac cagcgatatt gattgcaaat
      421 gcatctggac ttaatcataa gatgtcaaca ttattgattc aagccgcatt agtttttgca
      481 ggattatcaa ctttattaca attatatcca atacctttgt ttaaaggttt taaattagga
      541 ggaggaatac cagttataat gggagctaca gctatgttta taggagctgg ggtatctgtt
      601 gcaagtaagt atggactacc agtattattt ggctctcaaa taatagctgg tatagtaata
      661 attataattg gttttggtct taaaaaaata cgatttatat ttactccagc tgtatcagga
      721 acaatagttg catgtatggg tcttggactt tttccaatag ctataaaaaa cttagctggg
      781 ggtatgggca atgagacttt tgggaatcca attaatttta tagttggaat atctgtatct
      841 cttatgatat taggattaaa taagtatgga aaaggattat ttaaagatgc atcaatatta
      901 gttagcataa tttttggata cattttatca ttaattttgg gtatagtaaa tttttcttca
      961 attcaggagt ttactttggt tgcgcttcca aaaccacttg cttttgggct agatataaga
     1021 cttgaagttg ttgtaatgtt ttcaataata tatttagttg agatagctga tataatgggt
     1081 gcttgtacac tttctgcggt aggagggctt aatagacaag taacagatga agaactttct
     1141 tctgctgttt taggaaatgg agtactctct acaataggtg cattattttc tgcaactcca
     1201 atggggatgt ttggacaaaa ttctgcaatc gtttccaata caaaggttgt aagtaaattt
     1261 gtactagcta ttggaggaat tggattattt ttggcaggaa tatcgccttt attagctaat
     1321 ttaataagaa caattcctcc ctgtgtggtt ggaggagcaa cactcgttat attttcaact
     1381 cttacaacat ctggtttaag acttgttagt atggatggat ttaatcaaga aaatagcatg
     1441 atattaggtc tttctatggc ttctggtatt ggattcatgg tagctcctca agttcttgaa
     1501 aaatttccaa aatttataga gacattactt gcagattcaa gtgtagtttc aggagcaatg
     1561 gtagccataa taattcaagc tttatatatg gtaaaatttc ctggaaataa aagtgataag
     1621 gttgaggata aaaataaaag tttataatgt a
//
LOCUS       DAGQWR010000289         1648 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.294, whole genome shotgun sequence.
ACCESSION   DAGQWR010000289 DAGQWR010000000
VERSION     DAGQWR010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.294"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            227..1600
                     /locus_tag="MIW95_003908"
     CDS             227..1600
                     /locus_tag="MIW95_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3153175.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILANSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIEKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDILLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEGKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
BASE COUNT          732 a          161 c          281 g          474 t
ORIGIN      
        1 ctattggcat atttaatgac aattcaaata ttgtatataa ccataatgaa agatatataa
       61 ataaaatttt aaatatactt aaaaatatat taatataatg tacataaaaa atatcataaa
      121 tatatgataa gataaataca atagcaatat tttattaatt ttttgggttt ttatatattt
      181 aggtactggg gtgatatagt tgaaccaaga gatatttttt aaaataatga atgaaatatt
      241 ggaaaatatt gatgaagggg tacattttgt taataaagac gggaaaacta taatctacaa
      301 taattctgta caaaaaatgg aaaaaatgaa cagacacgat attttagcca attcattttt
      361 tgatatagtc aacaaaatga aaataaacag gagtacacta ttagaagttt tggaaaaaag
      421 ggaagttatc aaagacaata ttcaaaaata tctggacaaa aatggaaaag agataacagc
      481 aattaatact acaataccaa ttgatattaa aggcgagttt gtaggggcac ttgaaatatc
      541 aaagaatatg acaacgatag aaaatttact aaacagaaat tataaaaaaa atttagtcaa
      601 taaagttagt agtagacaaa aaaagggtta tgaatttgat gatattttag gggaaagtaa
      661 tttgattaaa gaagcaatag aaaaatctaa gaaagcttgc aagtctgatg cttcagtatt
      721 tatatacggt gaaactggaa cgggtaaaga gttatttagc cagtccattc attatggaag
      781 taataggaga aactatccat ttatagcaca aaattgtgcg gctttgccgg aatcactatt
      841 cgaaggtatc ctttttggaa ctagcaaggg aggatttaca ggagcaatag ataggccagg
      901 cttatttgaa caagctaaca aagggacatt gttattggat gagataaatt ccatgcctat
      961 tatgttacag gctaaactat taagggtttt acaagaaggt tatgtaagaa gggttggagg
     1021 aactaaggat atacctatag atgtaagaat tattgcaacg acaaatgaaa gtccaaaggt
     1081 aattttaaac gagcataaaa tgcgaaaaga tttatattat aggcttaaca taattcatat
     1141 aaaaattcct cctcttagag atagaaaaga ggacatactt ctattgtgca aaaaatttat
     1201 ttcaaagtat aataaaaagt taaataaaaa agtggtagga atttcagatg aagctattaa
     1261 aattttaaaa aattacgatt ggcaaggtaa cataagagag ctagaaaaca taatttattg
     1321 tactatgagt atgatggaag atgaggtaca aatagaaaca agtatgatta atctaaacga
     1381 ctattacaat cttaaagaga ataaagagag taacaattat tctttaaacg atttggaggg
     1441 caaaacactt agtaagatta tcagtgagat agaagaaaag tatatatatg aatcattaga
     1501 aaaggcatcg tacaatataa ctaaagctgc atcaatttta ggtatgaata ggcaaaattt
     1561 acagtataaa ataaaaaaat ataatatcaa aatattataa ataagcaaga taaaaaaaca
     1621 aaatagaagt aaaataaaaa agcaaaat
//
LOCUS       DAGQWR010000290         1539 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.296, whole genome shotgun sequence.
ACCESSION   DAGQWR010000290 DAGQWR010000000
VERSION     DAGQWR010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.296"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(73..423)
                     /locus_tag="MIW95_003909"
     CDS             complement(73..423)
                     /locus_tag="MIW95_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153176.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKTVTSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(646..837)
                     /locus_tag="MIW95_003910"
     CDS             complement(646..837)
                     /locus_tag="MIW95_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153177.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(956..1504)
                     /locus_tag="MIW95_003911"
     CDS             complement(956..1504)
                     /locus_tag="MIW95_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY3153178.1"
                     /translation="MGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKADIEPVL
                     LKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEERLSEFI
                     NGNTLKYDFVKMPHHGRYDKLTETFLESTSPQYAVITCSEKKEPEEGVLKILERLNIK
                     TFLTSSGEVVINSDGKTLSVNQ"
BASE COUNT          505 a          273 c          145 g          616 t
ORIGIN      
        1 tattatattt ttatatataa gtataatatt ctcaccttaa cattaattta tttcaatatt
       61 ttcatatatc ttttattcta aattcaatac ttttctaata tcttctatac cttgataaac
      121 ttcttgttta gaaaaattca tagcttctaa ttcttcatca gtcttttcca taagcatagt
      181 ataaaattct attgctatat actgcatatt ttcactagtt acagtcttag ctttatcaaa
      241 taaaatttct tctgcttcgt ttacatttcc ctgaaaaact aatcttttta aagttataag
      301 tataacttct tcttctgtat tatacactaa atctttttct tcatattcac tgtatttttt
      361 tcccaataaa agtcttgcaa ccataagttt tatctcattg aaaatcttat ccactatagc
      421 catttttaca tctcctataa ataaatttta ttttcttttt aattatatca gactttattg
      481 taaaaattat tatatgtaaa tgaaataatt caaaaataaa ttctaacaat aaaaaaaaga
      541 aggtagcggg ggatttccga ctaccttcaa acactagatt gtgattggtt tggtatttta
      601 tttataccca ccataagctt tattaaatca tctaaattga tttttttagc atccacatcc
      661 tccatcaaaa acgtcaccac attcacaaag tattaataat aatattaaga aaggtatcca
      721 tgcacataag tccatttcag cccaacaatc ttggaatgct aagaataaga agaataatat
      781 tattatccac caagcacatc caccaaatcc gccttcacca aaaaatctat ccaacataat
      841 atgttacctc ctagtcattt tgtatgtgga gtttcagctc ctaaaatata ctatgtttaa
      901 ggggtaatat ttgctaatga tttttttaaa ttttttttag atattttaaa aaaaattatt
      961 gattcacact caatgtctta ccatcactat tgataactac ctctccacta gaagtcaaaa
     1021 atgtttttat attcaatctt tccaatattt ttaaaacacc ttcctctggt tcttttttct
     1081 ctgaacatgt aataacagca tattggggtg aagtagactc taaaaatgtt tcagttaatt
     1141 tgtcatatct tccatgatga ggcattttaa caaaatcata ttttaaagta tttccattta
     1201 taaactctga caatctttct tcctctgcat ctcctgcaaa taaaaactta tgtgctccat
     1261 agcttatatt agtgattatt gaataatcat ttgaactctc gtacttgctt tttgaagccg
     1321 gatgtatgtt tatttctgca ctattgatta caatattcat attatctttt agtagcactg
     1381 gttctatatc agctttttta agtgagtcaa tatattcatc atattgctta ctatctttct
     1441 tataatttgc ttgtataata ttctttactt taacactaga taacacacta tctactccac
     1501 ctatatggtc cttatctaaa tgggttaaac tgaacctcc
//
LOCUS       DAGQWR010000291         1519 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.297, whole genome shotgun sequence.
ACCESSION   DAGQWR010000291 DAGQWR010000000
VERSION     DAGQWR010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.297"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            9..125
                     /gene="rrf"
                     /locus_tag="MIW95_003912"
     rRNA            9..125
                     /gene="rrf"
                     /locus_tag="MIW95_003912"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            133..207
                     /locus_tag="MIW95_003913"
     tRNA            133..207
                     /locus_tag="MIW95_003913"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:165..167,aa:Asn,seq:gtt)
     gene            213..298
                     /locus_tag="MIW95_003914"
     tRNA            213..298
                     /locus_tag="MIW95_003914"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:247..249,aa:Leu,seq:taa)
     gene            314..389
                     /locus_tag="MIW95_003915"
     tRNA            314..389
                     /locus_tag="MIW95_003915"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:347..349,aa:Met,seq:cat)
     gene            397..471
                     /locus_tag="MIW95_003916"
     tRNA            397..471
                     /locus_tag="MIW95_003916"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:430..432,aa:Glu,seq:ttc)
     gene            486..559
                     /locus_tag="MIW95_003917"
     tRNA            486..559
                     /locus_tag="MIW95_003917"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:518..520,aa:Gly,seq:tcc)
     gene            565..640
                     /locus_tag="MIW95_003918"
     tRNA            565..640
                     /locus_tag="MIW95_003918"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:598..600,aa:Val,seq:tac)
     gene            646..722
                     /locus_tag="MIW95_003919"
     tRNA            646..722
                     /locus_tag="MIW95_003919"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Asp,seq:gtc)
     gene            733..807
                     /locus_tag="MIW95_003920"
     tRNA            733..807
                     /locus_tag="MIW95_003920"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:765..767,aa:Gly,seq:gcc)
     gene            817..890
                     /locus_tag="MIW95_003921"
     tRNA            817..890
                     /locus_tag="MIW95_003921"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:851..853,aa:Arg,seq:tct)
BASE COUNT          471 a          270 c          328 g          450 t
ORIGIN      
        1 aaagattatg tggttacaat agcagagagg atacacctgt tcccattccg aacacagaag
       61 ttaagctctc tagcgctgat ggtacttggt gggaaactgc ctgggagagt aggacgtagc
      121 cacgttaaat gttccaaggt agctcaacgg tggagcaacc ggctgttaac cggtaggctg
      181 tgggttcgag ccccaccctt ggagccaaaa aagccgaagt ggcggaactg gcagacgcac
      241 aggacttaaa atcctgcggg acttacctct cgtaccggtt cgattccggt cttcggcact
      301 tataaaacaa tatcgcgggg tggagcagtt ggcagctcgt cgggctcata acccgaaggt
      361 cgtaggttca agtcctgcct ccgcaaccat taaaacggct cgttggtcaa gaggttaaga
      421 caccgccctt tcacggcggt aacaggggtt caattcccct acgagtcacc aattcaatta
      481 aatatgcggg tgtagctcaa tggtagagtt ctggccttcc aagccagctg tgagggttcg
      541 atccccttca cccgctccaa aaatgggacc atagctcagc tgggagagca cctgccttac
      601 aagcaggggg tcacaggttc gagccctgtt ggtcccacca ttcgcggcct ggtagttcag
      661 ttggttagaa tgccagcctg tcacgctgga ggtcgagggt tcgagcccct tccaggtcgc
      721 caaattaaat aagcgggaat agttcagtgg tagagcgcaa ccttgccaag gttgaagtcg
      781 cgagttcgaa tctcgtttcc cgctccataa taacttgggt gcatagctca gctggataga
      841 gcaacgccct tctaaggcgt gtgtccgggg ttcgaatccc tgtgcgctca ttaagaaaag
      901 tttactataa attatagtaa acttttatat tgtttaaaaa gacacaggaa acatgtatat
      961 tacctgtgtc tttttattta taaactttaa actatgtaaa aattacagta tattctaata
     1021 gagttacagt atattcatta atattgtaaa catacggagg gtaatgtata atagtctaca
     1081 agatgtttat gaaggcatca gttaaatata actaattaga taagagttat atacattata
     1141 ttgtatactt tatggtattt atatattgaa ataacttatg aaattattta taaagagatt
     1201 tatgagcata tttttgtcta aacaataagt ataagaaatt ataaaaaatt agagactata
     1261 gtgaattgac aatttaatgt aatatttata agttatacta agtaaattat tatactgtga
     1321 aatggatata taactttagg tattgtgtat tattttcact gtaggagatt atatctagtt
     1381 atacataata agttttaatg atagtttata gtaagatatt aaaataaatt tatctaatct
     1441 agatatgaaa taagttatta ctttaatata aaaatgaatc taatggaata atattgatga
     1501 taagtaatat ttagatttt
//
LOCUS       DAGQWR010000292         1513 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.298, whole genome shotgun sequence.
ACCESSION   DAGQWR010000292 DAGQWR010000000
VERSION     DAGQWR010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.298"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            64..693
                     /locus_tag="MIW95_003922"
     CDS             64..693
                     /locus_tag="MIW95_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY3153179.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQSY"
     gene            668..1072
                     /locus_tag="MIW95_003923"
     CDS             668..1072
                     /locus_tag="MIW95_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBY3153180.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            1075..>1513
                     /locus_tag="MIW95_003924"
     CDS             1075..>1513
                     /locus_tag="MIW95_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBY3153181.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQD"
BASE COUNT          635 a          175 c          221 g          482 t
ORIGIN      
        1 aaaattaaat tatacaataa cagtgtaaat taacttaaca ccgttaagca aagaggtgat
       61 tatatgagta tcaaagaaag acgtaaaaat gaaaaagaag aaatgaagaa aaagattatg
      121 gatgctagca tcgaaattat aaaccaacat ggatatgaaa atttatcaat aagaaaaatt
      181 gctacaaaaa tagaatattc tccaactaca atttatcttt actacaagga caaagcagag
      241 attatcagcg atatgaccaa taaactttat aacacagtag aaagtaatgc tattgacatt
      301 atgaataatt gctcatcact accaatagat aaacaaatca tagaaattat gactagcttt
      361 ataaaaactc tttctagtga accagagatg gcaaaagcta tcatgcatag tagaatgaat
      421 attatatttg catctgaaaa tacaaacaat actcctagca ataatggtat aaaaatgctt
      481 gataaattcc tttccattgg aattgaacaa ggaatattca aaaaaaatat agataaaagc
      541 tcatggatgc ttataagtgc attgttagga ttcgttttat gtgttgttga aaataaacta
      601 tattctttac ataatttttc tcaacttata gaaaatttct tagatatttt agtgggagga
      661 ctttacaatg aaaattcgca acaaagctac taataaaaag aaaaaatatg taaaaatatc
      721 tattgctcta ttctttgtat ttatatgcat tattattgta attgtaagca acttaaagcc
      781 agaaaacttg gtagtaaaag atatagatat taataatgtt aaaaatggca tttatactgg
      841 aagtgctgat aataacttgg taaaagctac tgtgtctgtt gaagttaata atggtaaaat
      901 ccaaaatatt aatatcttaa aacacgacca tcttttaggt aaacctgctg aaaaaattac
      961 aacaagtatt attaaacaac agtctttaga tgtggatgct ataacttctg caacttatag
     1021 cagtaatact atacgaaaag ctgtagaaaa tgctttaaga aagggagaat aattatgaat
     1081 actattatta tatattcaag taaatatggt tgtacaaaag actgtgcaaa tatactaaaa
     1141 aataaactat cagataatgt cactattgtt gatattaata ataataataa aattgagtta
     1201 tcaaaatttg ataaaattat tattggaagt tctatatatg ttggttcagt ttcaaaaaaa
     1261 atacaagtgc tatgtaatga taatgtagag ttattaaaca aaaaacaagt tggcattttc
     1321 ctttgctgtg gcttttctga acaagcagat aaatatttaa aatcaaattt tccatcatcc
     1381 ttattagaaa gtgcaaatgc tattggaatt tttggtagtg aagctcgatt agaaaaaatg
     1441 aaatttttag ataaacttat tatgaaggct gtttcaaaag gtaattatga cagttttaga
     1501 atatcacagg ata
//
LOCUS       DAGQWR010000293         1483 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.299, whole genome shotgun sequence.
ACCESSION   DAGQWR010000293 DAGQWR010000000
VERSION     DAGQWR010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.299"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..151
                     /locus_tag="MIW95_003925"
     CDS             <1..151
                     /locus_tag="MIW95_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY3153182.1"
                     /translation="EVENMLKIENIQYEFTSRDGFIFFGFYEMTDAYRFETLCSDEKI
                     RFKRI"
     gene            complement(711..1034)
                     /locus_tag="MIW95_003926"
     CDS             complement(711..1034)
                     /locus_tag="MIW95_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153183.1"
                     /translation="MATEIFTVKITPLEAQKMIKENIDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTEKTVIKATSTGSAIGVLKIDWGAGGNLLKRVKNIL
                     AEYII"
     gene            complement(1079..1333)
                     /locus_tag="MIW95_003927"
     CDS             complement(1079..1333)
                     /locus_tag="MIW95_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153184.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
BASE COUNT          536 a          205 c          127 g          615 t
ORIGIN      
        1 cgaagtagaa aacatgttga aaattgaaaa tatacaatat gaatttactt ctcgtgatgg
       61 ctttatattt tttggttttt atgagatgac agatgcatat agatttgaga ctttatgttc
      121 agatgaaaaa attcgattta aaagaatata ataaataaaa aaagtacata tttctaaact
      181 atttgttttt caaatttagg ttgtgtttta atgtagtgtt gatatcataa acaattttaa
      241 taataaaaaa gtaggaatat tatattaata tgctcctact ttttactcat atttattaat
      301 attatttatc aaaactaccc tacaaactgg aatttatcat tctttcataa ttttaaacta
      361 tcatatttaa aatatataaa attctacttg atttttgtat aaacgtaata tcaatcatta
      421 tccaatttaa aagtattttt tagtaatttc catttcattt tcttttaaca tattaattaa
      481 acttttatat gtaaatggtg tatttacata ttatttaata tccaacaatt atataaagaa
      541 atgtactctt tgccttataa agaattataa agccaattcg ttttattata ttatttttct
      601 ttatacatta aaaactttac cttcaaatcc ttatttatct atatattata aaaatattct
      661 taacctcttt tacgagattt tcttgacata ttatcagtta gataaaataa ttaaataata
      721 tattcagcaa gtatgttttt tactctttta agtaaatttc cacctgcacc ccaatcaatt
      781 tttaaaactc caattgcact gcctgttgaa gtagctttaa taactgtttt ttctgtggta
      841 ttttcataaa ctacgattaa tcctgcatca ctattatttc ttgcgtaata cttctcaaaa
      901 gttgtaatta gtataaattt atcatctcct aaattataag aatcactaaa aactaaatct
      961 gcatcaatat tttcttttat cattttttgt gcttctaagg gtgtaatttt tacagtaaat
     1021 atttctgttg ccatttttta tctcctcata agaatttatt ttagacaaaa cataatctct
     1081 agtattttct gatatattta tcaaacaata tccctattcc tcctaacaga gctaatatag
     1141 aaccaaaatt tctatattga gaaatagatt ggtctagata aatattatat tggtcagtat
     1201 ccatgattcc accattttgt tgaataatat gctctgcact aaattcaccc aaacctatac
     1261 agcagaataa tactactact ccaattacaa aagcaaatat acatattgtt aatttaaaat
     1321 ccttattttc catagttatc cctctcataa gttaaattta tttgattatt tttactagat
     1381 tttatattac tcttattata caatagaaca tatatttaca tctatttcca aaattgtaac
     1441 ctttatagtt ttatatattt aaatttttta aacaaaaaat aca
//
LOCUS       DAGQWR010000294         1479 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.300, whole genome shotgun sequence.
ACCESSION   DAGQWR010000294 DAGQWR010000000
VERSION     DAGQWR010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.300"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            159..968
                     /locus_tag="MIW95_003928"
     CDS             159..968
                     /locus_tag="MIW95_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3153185.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKEKL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEV
                     QNNKRPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            1011..1277
                     /locus_tag="MIW95_003929"
     CDS             1011..1277
                     /locus_tag="MIW95_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBY3153186.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTDIEKPFWGEQCPVKSCCED
                     KNLIHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
BASE COUNT          585 a          165 c          202 g          527 t
ORIGIN      
        1 atttataata attgtatatt ttgagatttt actttacaat tattgtatct aaatttaaca
       61 aaatatctta ttagaatatt tcaattaaaa caaaactggt ttattatata aaaaataaat
      121 tagtatactt attttttata tatcaggagg atatacttat gaataaaata atcaataaca
      181 ttttaaatac ctgcaaacag tatacatata ttttgccaca taaattgtta agacagtata
      241 tagcacacta tacaatatct attccagata caagtattaa agaaaagctt acattaatac
      301 ctgatgctag tggatgtatg atatttaaat ttgacaaaaa aggtatagaa agcgcattct
      361 ggggagccac aacaaaaact actattgtaa aaaatgatat agaaaatgta ctttttagag
      421 tattcgttga gtttcgccca ggtggagtat attatttgac aggtctttct caaagagaat
      481 ctacagattt aaaaatttca cttgaagatt ttaacacatt atttagctta gaggttaatt
      541 ccatttttga aagaacaagt actataaagg aattagtaga acaattagat atgttattct
      601 tatcatactt attgaaatca aatatagtag atatgactat accaattctt gaaaatgcta
      661 gaaaacagaa ctctattatg tctgttaaaa acatttcaca aattagttgt tatagtgaac
      721 gacatttaaa tagaattttt aataattctc ttgggatgag tgttaaatca taccttagat
      781 tacttcgtat aaaccttgta ttacaagaag tacaaaacaa taaaagacca tttgcaactt
      841 tagcacaaga tattggttat tacgaccaat ctcattttat aaatgatttt aaatctattt
      901 gtggtgttaa tccaacaacc tatataaaaa atttgtccga tttttacaat gaaaagtata
      961 aattttgata tacaatcata ataaaattta tatttaagga gtgacttaaa atgatagaat
     1021 caagatgtgg aattttatgt tatgaatgtg gatacaagga aaaagtcaat tgtaaaggtt
     1081 gtacagatat tgagaaacca ttttggggtg aacaatgtcc tgtaaaatct tgttgtgaag
     1141 ataaaaacct tatacattgt ggtctatgtg atacatttcc atgtgaaatg cttaatcaat
     1201 ttgcatatga caaagaacaa ggtgatgatg gcaaaagaat ttctcagtgc aaaaaatggg
     1261 ctgtactagc cttataatat actctcttgt tataaaataa gtttttgtaa ggtaaatttt
     1321 ttaaactgtt gtagaaattt ctgcaacagt ttttataatt aaatttaaag gttatttaat
     1381 taaattaata gaatagtaat ttattgataa aatctgatta ctaaaattag aaatataaaa
     1441 tctcaatctc tgtctattta aaatatatgt tacaaaatt
//
LOCUS       DAGQWR010000295         1405 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.301, whole genome shotgun sequence.
ACCESSION   DAGQWR010000295 DAGQWR010000000
VERSION     DAGQWR010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.301"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(419..910)
                     /locus_tag="MIW95_003930"
     CDS             complement(419..910)
                     /locus_tag="MIW95_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBY3153187.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDENIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          544 a          179 c          125 g          557 t
ORIGIN      
        1 ttttctaaag attttaattt ttctgttttt aattaaatta aagtatcttt gtttctgaaa
       61 ttttttaagt ttatgaagct ataatttaat atatatttat tttatttgtg agtctatttt
      121 tatatataat gaaaaatgtg ataagtatcc aaaagaatta ctggatataa taagataggg
      181 tatttagcct tttaagataa ataaaaatat ctgcaactat atattgcaaa aatttaaaac
      241 taatttatat atgctaatat aaatatttat aaagacataa gacaaattta cctacagatt
      301 taaatttaac ttatatattt taaaaggcta atataatatg tattttcttc tatacaatca
      361 aaaggcttca tatctaagat atgttatgac actaatattc acaatttttt agtaattatc
      421 acattataaa aaatattaca gctagtccat catcagatgc ttcttgtata aactttttaa
      481 gctcaattag atttgcccat acatattcaa aaaaatcatc ttcactctca tcattcataa
      541 caggataaat attattttct aacatctcat taatattaaa tctttttcta aatgcttgtt
      601 cttccaaact gttcattgct tttgatagtt tttttacatc atttgctgta atcaacatga
      661 caggaaattc tccatcaatt tccataagta tgtttccact tagtactaat ttgctaaata
      721 tattctcatc atctccacca aaaggacaac ctgtcaaagt aaagtgaatg gcatgccatg
      781 ctttatcaat attgatagta ctattttcgt tggcatcttc aaaaacaaaa tcttgtaaat
      841 tttcttctgc ttgctgtaat ttaaaaacat tctcttcact tattttagta taacatccta
      901 tcatccccat aattacattc ctcctaagta gcgttttttt aattgtaact tataattaaa
      961 cttagttaca ataaaatata ttgagaaaag tattatttca tcttaaattt aaatacctat
     1021 attatcaact aaaattctgt gtttatagcg taagtattat tattttttcc taaaaattaa
     1081 atgaatcaga agttctatag gcaatattga ctataaataa gaactattac ttttaaaaaa
     1141 aatttaagaa attatactgt agaatcatgc tctacttatt agcttcaaat aaaaaataaa
     1201 tcatgttctc aaattaaata ataaataaca ataaatttaa attttaaact ataagagttt
     1261 ctgaagtgtt aataagcata ccaataatta tatgtgtaag aatttgattt aatagatatg
     1321 aataattttt acattaatta taaatatatt aacctattat tataaattct ataaaaagaa
     1381 aagttatctt aaataaaaaa taagt
//
LOCUS       DAGQWR010000296         1381 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.302, whole genome shotgun sequence.
ACCESSION   DAGQWR010000296 DAGQWR010000000
VERSION     DAGQWR010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.302"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            256..1296
                     /locus_tag="MIW95_003931"
     CDS             256..1296
                     /locus_tag="MIW95_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY3153188.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT          562 a          134 c          251 g          434 t
ORIGIN      
        1 aataagaact taaattaaaa attttaaaaa ttattttcat ataaaatatg gcattgtaat
       61 tgcttattta atagaggatt tttgtaaaat gaagaaagta tatctatcaa attaaaacta
      121 agtagatata ctttttatta agttttgttt taaaataata ttattaaatt ataactaagc
      181 ttttctgtta tgatataatt aaatagataa tgtgaatgat tattatatag aatacttatt
      241 tggagttgat attttatgga caaacacagt gattcagtaa tgtatggtgg aatggaagta
      301 tataatattt ggggaacaac aacagtagac tccataattg gagagtctga tgtaatgcgt
      361 gctcttaaga atagaatttg caaaattgga aatagtgatt ctactgtagc tataactgga
      421 gaaagtggca ctggaaaaga attaatagca agagccattc atctcgcagg aaacagaggg
      481 aataaaacat tcgttgctat aaactgtgct gctataccag aacctttact agaaagtgaa
      541 ctttttggat atacaaaagg agcttttagt ggagcagatt caaaaggtaa aattggtaag
      601 tttgaattgg ccaatggagg ggttgtattt ttagatgaga taggagatat gccaattcaa
      661 ctacaatcaa agttattgag ggtattacag gaaaagaagt ttacgagaat aggttcaaat
      721 gaactaattg atatagatgt tagaatcata tcagctacta atagagattt atttgagttg
      781 gttaaggaaa ataaatttag agaagactta tactatagat tgaatgtaat acctttagaa
      841 gtgcctccac ttagagaaag aggagacgat atacaaatat taataaataa ttttatagac
      901 aaatattcga gaaaaatgaa gaaaaacgtg taccatatag aaccagatgt ggaacaaatg
      961 ttgcttaagt atccttggcc aggaaatatt agagagttgg aaaatactat agaactggca
     1021 ttaaaccttc ttgaggaaga tggaataata cataaaggaa taataaatag aaagatactt
     1081 gaatatttta agttaaataa tataaatata aagttacttg aggaaagtga ctatgagata
     1141 agtatggaac aagacatact tacacttgaa gagtttgaaa gagcctatat aaagaaagtg
     1201 ttaaagtttt acggaaatga tactaagtct aaaaaactgg ctgccaaaaa acttggaata
     1261 tcactagcta cattgtacag aaaaatagat gtataataat taggtttttt attctcaatt
     1321 ctaaaatttg aaattataaa attgataaaa aacacttgga aaactatttt ctaagtgttt
     1381 t
//
LOCUS       DAGQWR010000297         1357 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.303, whole genome shotgun sequence.
ACCESSION   DAGQWR010000297 DAGQWR010000000
VERSION     DAGQWR010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.303"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            71..1180
                     /gene="ugpC"
                     /locus_tag="MIW95_003932"
     CDS             71..1180
                     /gene="ugpC"
                     /locus_tag="MIW95_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBY3153189.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDKIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            1210..>1357
                     /locus_tag="MIW95_003933"
     CDS             1210..>1357
                     /locus_tag="MIW95_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HBY3153190.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNN"
BASE COUNT          560 a          174 c          209 g          414 t
ORIGIN      
        1 ttttacacat ttaatataat aaaaaaattt gatactatat cattaaaatt taaatatcaa
       61 ggagaatatt atgtcagaag taattttaaa aaatatatca aaactttact caaatggatt
      121 taacgcagta aaaaatataa atatagacat taaagataaa gaatttatcg ttttagtagg
      181 accatctggt tgtggtaaat cgactacact tagaatgatt gctggtcttg aagaaatctc
      241 tgaaggagaa ctatacattg gagataaact tgtcaatgat atagaaccaa aagatagaga
      301 tatagctatg gtttttcaaa actatgcttt atatccccac ttatctgttt acgaaaacat
      361 ggcatttgca cttaaactta gaaaattgcc taaagatgaa atagacaaaa aagttaaaga
      421 agctgctaaa atattagact tactacctct tttaaataaa aaacctaaaa cattatctgg
      481 aggacaaaga caaagggttg ccttaggacg tgcaatagtt agaaatccta aggtattctt
      541 aatggatgaa cctctatcca acttagatgc aaaacttaga actgctatga gaacagagat
      601 aactaagcta caccaacagc ttggaactac atttatctat gtaacacacg accaagttga
      661 agctatgact atggctgata gaatcgtagt aatgaaggat ggagtagttc agcagattgc
      721 aactccacaa gatgtatacg attatccagc taatatattt gttgcaggat ttattggagc
      781 accacagatg aattttattg atgtaatatt aatagaagaa aatgataaaa tttatgcaca
      841 aaatgaacat attaagctta aattgaataa agaaaaacac tatgatttaa taaaagacaa
      901 ttatattaat aatgaagttg ttataggtat cagaccagaa gatattcatg tagaagatac
      961 ttttattaaa tctaatccag atacttgttt taaatcaaga attgaaataa aagaacttat
     1021 gggagcagaa acttatgctc acttaaaact aggcgaaaat acgataacaa ttagatttga
     1081 cagtaaaaac agaattaatg ttggtgatga cttaacactt tcagttgaca atagtagagt
     1141 acacatattt gacaaagaaa ctactttagc tataagataa gaatataatc ttatttttaa
     1201 ggagacttta tggaagagtt aaaagatttt ttagaaagac agcttaataa aaaaataaat
     1261 atctatccct atgaaaacca aaagttagat gctagtagcc atttagttac tttcaataat
     1321 gctatttatg ttattgattt tctagataaa aataatc
//
LOCUS       DAGQWR010000298         1255 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.305, whole genome shotgun sequence.
ACCESSION   DAGQWR010000298 DAGQWR010000000
VERSION     DAGQWR010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.305"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            18..>1255
                     /locus_tag="MIW95_003934"
     CDS             18..>1255
                     /locus_tag="MIW95_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBY3153191.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKVDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLV"
BASE COUNT          537 a          163 c          154 g          401 t
ORIGIN      
        1 gataaaagga gtattttatg aacatacttg gattaatagt tgaatataac ccatttcata
       61 atggacacat atatcattta tctaaatcta tagaaaaaac aaatgcaaca catactgtag
      121 caataatgag tggaaatttt ctacaaagag gcgaaccagc tttatttgat aaatatacta
      181 gagcaaaaat tgctgtcgaa aatggtgtag acttagttat agaattacca actttatttg
      241 cttgtcaaag tgctgaaatt ttttctcatg gtgctataac aattttaaat tcactaaact
      301 gcataaattc tatatgtttt ggtagtgaag aaggaaatac agaaattctt tataatatag
      361 ctgaaatttt agcaaatgaa ccaaatgaat ttaagttact tttaaaaaaa tatttagatg
      421 atggcctgtt atttccaaca gctagagcta atgcactttt tgattatata catcaagaca
      481 aaaaaaataa ttttatacaa aattattcaa aggaacaatt atcaaaaaca ttaaattcat
      541 caaacaacat actgggtata gaatacataa aaaacctcat ccatttaaac agtagtataa
      601 aaccatttac tatctccagg gtccaatcag aatataattc cgaagaaatt gagagtgata
      661 tttgttctgc tactgctata aggacttcac ttaaagaaaa tacagacctt tttcatttaa
      721 aaaatgttat tccagaaaaa acttataaca tcctaaatga aaaagtagat aatggttttt
      781 tacctatgtt tgatgacttc ttctttgata ttttaaaaac aattatactt agagatatgg
      841 acactttaaa agagtatttt gaagtaaatg aaggcataga aaacagaatc tataaagata
      901 tctttacatc tgaatttcta catgagctac aattagcaat taaatccaag cgatacactt
      961 taaccaaaat caaacgaact ctaaacaata tattgttagg tattaaaaaa agcgacatgc
     1021 aacttataaa aaacatagat actatacctt atataagaat acttgctttt aatgataaag
     1081 gtagagaaat tctaaaagag ataaaaaata aatctgaaat aaaaataata aacaagtttt
     1141 caaatatatc attttcatta gatgatacga tttttaaaac actcataagt tatgatataa
     1201 aagctacaaa tatatataat tcaatatatt ataaaaacaa taaagattta gtaaa
//
LOCUS       DAGQWR010000299         1253 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.306, whole genome shotgun sequence.
ACCESSION   DAGQWR010000299 DAGQWR010000000
VERSION     DAGQWR010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.306"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            288..587
                     /locus_tag="MIW95_003935"
     CDS             288..587
                     /locus_tag="MIW95_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153192.1"
                     /translation="MDVEQSKNVYLRIGKELSEKMTKSELTKFSQEVANYLQYAGLDK
                     NMKIKLFQTIIEAVARKKVSLPAGSEFTQGIMLSNELEMTTNVGFIVEGLSYHNN"
     gene            complement(758..1234)
                     /locus_tag="MIW95_003936"
     CDS             complement(758..1234)
                     /locus_tag="MIW95_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153193.1"
                     /translation="MDKKEAKKFLKVVDMNIDKIKEEATKIYKECYLIETTDTIRISI
                     NLKGVVKSTISSWQGYSDDIFNMREIIVYEFSQGEVQIEDLLGELCFLNDYEEFATWC
                     DTESETLNWNSYEKFNKENFDELVERNIEDSLPDFLEELSESIENCKQELKSMIEI"
BASE COUNT          456 a          177 c          146 g          474 t
ORIGIN      
        1 tattataaat aatcaacata gttattcaaa gaaatgaaaa agcactctcc acaacgaaga
       61 atgctctata taataatgtt tgactcagta aatatgcgtt ggggttgcat atctactttt
      121 ttattatatc attaattttg tgcatcaaaa agagctattt aaatcaattt taaggtgtgt
      181 tgagtgatgt tattgatgtt tacatactta aacaaaaata tttatatttg aagtattagc
      241 atttttaaaa tataatttaa ttataatcta aaaaggggtt gaaaaaaatg gatgttgaac
      301 aatctaaaaa tgtttatttg cgaattggaa aagagttatc tgaaaaaatg actaaatcag
      361 aattaactaa attttcacaa gaagtagcaa actatttgca atacgctgga cttgataaga
      421 atatgaaaat taaactattt caaacaatca tagaagcggt tgcaagaaaa aaagtatcat
      481 tgccagctgg tagtgaattt acgcaaggaa taatgttaag taacgaattg gaaatgacaa
      541 cgaatgtagg atttatagtc gaaggattaa gttatcataa taactaaaac tttagcatta
      601 aaaaagcagt gtatttattg aatacactgc ttttatttcc gtatagttaa tctaaaattg
      661 gatattatat catttcgata taaattaaat tgacatttaa atccctcgta gttaatcaaa
      721 agcgatacta tatacattaa taataatact ctttcattta aatttcaatc attgatttta
      781 attcttgttt acaattttct atactttcgc tcaattcttc caaaaaatct ggtaaactat
      841 cttctatatt tctctcgact aattcgtcaa aattttcttt gttgaatttt tcataactat
      901 tccaattcaa agtttcgctt tcagtgtcac accaagtagc aaattcttcg taatcattta
      961 aaaaacaaag ttctcctaaa agatcttcaa tttgaacttc tccttgacta aattcataga
     1021 caataatttc tctcatatta aaaatatcat ctgaatagcc ttgccaactt gaaattgtgc
     1081 tttttacaac tcctttcaaa tttatagaaa ttcttatagt atcagttgtt tctattaagt
     1141 agcattcttt ataaattttc gtagcttctt cctttatttt atctatattc atatctacaa
     1201 cctttaaaaa ttttttagct tcttttttat ccattttacc cattttaatt tcc
//
LOCUS       DAGQWR010000300         1223 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.307, whole genome shotgun sequence.
ACCESSION   DAGQWR010000300 DAGQWR010000000
VERSION     DAGQWR010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.307"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(174..884)
                     /locus_tag="MIW95_003937"
     CDS             complement(174..884)
                     /locus_tag="MIW95_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3153194.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELEKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVHIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
BASE COUNT          446 a          161 c          128 g          488 t
ORIGIN      
        1 aattaagttt caaaaaaact ctaaaaacta agcttcaaaa aattaaattt tgaaagattg
       61 aaattctaga aattaagctt caaaaaatta aatttgaaaa aacaaaactc taaaaactga
      121 gctttaaaaa attaagtttc aaaaataaaa ttctagagat tgaacttcaa aaattaaggt
      181 tctaacatat accttgtaaa ttttcctaca acctcttgat attttttacg gcttatagga
      241 attttttcat tctgaacttt ggcataagtt tgagttatac tatctacatg gtttagattg
      301 acaatatacc tttgatgcgt acgtataaaa ttagagggaa gaacagtttc catatcattt
      361 aattttccgt agtattctaa tgttttatca aaggtaacta cagttaattt tcttagctcg
      421 cttttaaagt agaggatatc ttttgtatta attttacaaa gattagttcc aatatgaact
      481 gtaataaagt tttctttcca ttgttctaat ttttttagtg cttcttttaa tactttacat
      541 attttttcag ttttataagg ttttaagatg tagttcaaag ctcctacatc atacccatga
      601 aatacatagt cagaaaaacc tgtgacaaag atgattatag tatcttttga atgttttcgt
      661 atagtttttg ctatgtcaac gccattactt ttttcaagtt caatatctaa aaaaataata
      721 tctatgtaat aattttcgtg ttctagattg tatactagac tttctccatt tgaatattct
      781 tttatttggt aggggtagcc ccacaaatcc atttctcttg taatgatttt attcagcatg
      841 tttctttgtt gtttttcatc atcacaaata caaatcgaag tcaatttttt atctcctttc
      901 aagttttata tttatatagt taagtatata atatattttg ctacctagat atataaaatg
      961 aaataacaat tatatccaga tagcaaaata aaatttttta tattagtata cttacactta
     1021 aaactattct attaaactta ttctgaagtg ctaatgattt tcttattaaa aaatctaata
     1081 acctttctta ttataaaata ccaatgcttt tattattata aaaatgtaat gacttttcat
     1141 attataaaaa gcaaatgatt atcttactat aaaaatacaa tgacttttaa tactataaaa
     1201 agcaaataat tatcttacta taa
//
LOCUS       DAGQWR010000301         1221 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.308, whole genome shotgun sequence.
ACCESSION   DAGQWR010000301 DAGQWR010000000
VERSION     DAGQWR010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.308"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            287..1039
                     /gene="cwlD"
                     /locus_tag="MIW95_003938"
     CDS             287..1039
                     /gene="cwlD"
                     /locus_tag="MIW95_003938"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBY3153195.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          540 a          116 c          176 g          389 t
ORIGIN      
        1 aaaacatatg ctaataacat ttaaatttta tttttataat attgtaattt aaaagtttat
       61 ataaggttat atattttaag atattattta acaatataaa ttctgaatat acgttatcaa
      121 agtttataat ataaactaag tataatttat tgtatagtat tgaatgttag ataaacatag
      181 tgaattaaat aggggcagaa atataatatc attacataat aatatattaa taccaataaa
      241 taaagatata tcaatattaa aaaaatttaa atgtttgtac taatatatgg aatattatag
      301 tgtttttata agtatacata tacagggtga tgttgtgaga aagtacataa aacatataat
      361 ttttagtttt gcaatgatat gtctagtagt agtatccata tttgaaatta aaaatatttc
      421 tgaagatgtt atcaagtata tgccagtaac aaacaaaact ataattttag atgctggtca
      481 tggaggcatt gatccaggtg cattaaataa ggataagagt acatctgaaa aggatattaa
      541 cctagcaata acacttaagc ttagagagct tatagaatca agtggtggcc ttgtaatatt
      601 aactcgagaa gatgacagta gcctttataa agaagaaaat aataaaacta caagacaaaa
      661 atataatgag aatttgaaaa atagaaaaga aataatatct aattctaatg ccaatatgtt
      721 tgtctctata catcttaatg catttgaaca atcaaaatac tatggagctc aaacttttta
      781 tcctaaagac aaacaagaca gtaaagaatt atcgaagtgt attcaagaag aacttaaaag
      841 agtagtagat aagacaaaca acagagaagt caaaccaagg gatgatatat atcttttgaa
      901 agaaaataat attccatcag tactgataga atgtggtttt ttatcaaatg aaaaagagtg
      961 taaactctta actgatgaaa catatcaaga aaaaatagca tgggcaatct acataggaat
     1021 acaaaaatat ttaagttgaa ataagtataa aaataggtct aaatatgtaa atagcttgta
     1081 ttaaagcagt taaaatgcat taatataggc tatttttatt ttgacaaaaa aatatttaaa
     1141 ataaaagtta aaaagttgtt gacttagaat aatatagatg atattatata tgagtgccca
     1201 aaaggagcac caaaataaga c
//
LOCUS       DAGQWR010000302         1181 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.309, whole genome shotgun sequence.
ACCESSION   DAGQWR010000302 DAGQWR010000000
VERSION     DAGQWR010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.309"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            101..358
                     /locus_tag="MIW95_003939"
     CDS             101..358
                     /locus_tag="MIW95_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153196.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(468..1148)
                     /locus_tag="MIW95_003940"
     CDS             complement(468..1148)
                     /locus_tag="MIW95_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBY3153197.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          491 a          145 c          132 g          413 t
ORIGIN      
        1 aaataaaata ctaagtttaa aaatttaaat aatctataaa tcaattataa aatattattt
       61 cttagaaatt aaaatgaatt aagtaaatgg aggaattgat atgataataa ttagaagtca
      121 agataaaact aatttaatgc agataaacca aattaaaata gatggtagtc aagtttatgc
      181 agtatttgaa agtaaaattg atactgtaaa aataggagat tatgaaaata atacaagagc
      241 tatacaagtg ttggacaata tacaaaactt cattgaaaat ggaacaaaat atgattatat
      301 aacatcaaat aaagtcagat acaatgttaa taaaatattt cagatgccag ctaaatagtg
      361 agtgtgcatt ttagtttaat aactaaagcc cactttcttt tgtagtatga aagagggctt
      421 tacatatttg taaatatcga agtacacttg ttttatcaat aaattattta ataaggtata
      481 atggaccaat taaacaataa aaattctcct aaaacagcaa gtattaaata tataatagct
      541 tcatttttgt aaagagaggt aatttttttg cttatgtcat catcaattgt aatagcaatt
      601 tcatcttgaa taatattttt atatatagat gggaaaatat cttttttctt tataagatta
      661 aataaattat ttacacctac ccatataaaa tatcctaaaa cagcacctaa tgtgttcatc
      721 attaaatcat caatatctgt agctcttaag ttaaacaatt gacaaagttc aattgctaat
      781 gaaaaaccta atccagttaa tgaaacattg ataatatttc taaaattttt ccaaattaaa
      841 ggtagtaaaa atcctaaagg cataaacata attatattta aaatatatgt taagatgcca
      901 actgagtcaa aaggtattag atttatttca tcaattctaa aaagcccact ataccttcct
      961 atatcccata tgctaccaat tccagcttta ctaagtgcta gatagatata taataaaaaa
     1021 atatataccc atactaaatg tatcattaca ttcttttcaa ctttcaaatt ttttcgtttt
     1081 ataacgacta tttggtatat taagcatggt aatataacgc aaataaatga ataaaatgtc
     1141 catatcattt tattttgtcc cctttcaaat ttatgttttt a
//
LOCUS       DAGQWR010000303         1170 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.310, whole genome shotgun sequence.
ACCESSION   DAGQWR010000303 DAGQWR010000000
VERSION     DAGQWR010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.310"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(310..510)
                     /locus_tag="MIW95_003941"
     CDS             complement(310..510)
                     /locus_tag="MIW95_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBY3153198.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(735..911)
                     /locus_tag="MIW95_003942"
     CDS             complement(735..911)
                     /locus_tag="MIW95_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153199.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(965..1054)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          439 a          166 c          114 g          451 t
ORIGIN      
        1 ttaacaaatt actaaaagtg ttatatcaga attgaataca aaagaaagag tatgtattta
       61 tgagatagtt gaaaattttg atgtgccaca actaaaatta ttatattact atatattcta
      121 attaaaagag tacttgtagg tttaagtaca tactttataa atatattttt gtcagattta
      181 ttttataaaa agtatttaga ctaagtatta tttttaagta ttttcttgtt gaagttattt
      241 tttccttttt tacaaaatgc gtaaataaaa aaaagactct taaaagagtc tttccgaaaa
      301 atctatatat tataatatag ttatattttc tgcttgagga cctctagcgc ctttaactat
      361 atcaaagctt actttttgac cttcttctaa tgatttaaat cctgaagttt gtatagctga
      421 gaaatgagca aatacgtcat ctccactttc cattgatata aatccaaatc ctttttcatt
      481 attaaaccat tttactattc cattattcat taaaaatact cctattaacc tatatcaaga
      541 tatacttgat ttacttgtgt attaccacaa atactattat atcatagatt aacataaatg
      601 tctatttaat tataattttt agttaacatt tttatttatt cttaatatga aaataaattt
      661 aaacatttta tattaccaat atattaatgt aaatgtagat aaaactaaaa agggttttct
      721 agaagaaaat ctgtctaatt atacttactt accaatacca tcacttgatg caagcaagat
      781 tccacaactt tttctccgta ctctaatata aaatcatcta actgtgtatc tataaaatca
      841 caataatcat atgaactacc aatataagca gttagcatat gtgtacattt atccatttta
      901 tctctaaaca taatgtattt atccccctaa aaaactttca tgaaaaacaa aaagaaaata
      961 tctttttggc aactggctga cataacttat tttataagcg ttagtcccta tagctttgcg
     1021 tcactaaatt tctctagttt tgccgattta gttttattct acagattaat aatacaatag
     1081 aattagctat tattcaacat atctagctga aaaattaata aatttatata tgaacgttaa
     1141 attatttgaa aaaataaaaa gcccctctgt
//
LOCUS       DAGQWR010000304         1153 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.311, whole genome shotgun sequence.
ACCESSION   DAGQWR010000304 DAGQWR010000000
VERSION     DAGQWR010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.311"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            42..419
                     /locus_tag="MIW95_003943"
     CDS             42..419
                     /locus_tag="MIW95_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY3153200.1"
                     /translation="MKEEILIEKLLRAELIVMEYLCKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINFQYHYKIIIDNKEYYTFKKKVLKSSKSIKIMRSLT
                     TTHKNVSKEKLDLLDEYYRNLKE"
     gene            524..899
                     /locus_tag="MIW95_003944"
                     /pseudo
     CDS             524..899
                     /locus_tag="MIW95_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          537 a           79 c          174 g          363 t
ORIGIN      
        1 attattaatt taagttagaa gaattaagga agtgacataa attgaaagaa gaaatattaa
       61 tagaaaaatt attaagagca gaattaatag taatggagta tttatgtaaa aaagacactc
      121 taatgcctaa aaaagaaatt gtaaaagaaa taaaacagat ttatggatgg cataaaagta
      181 caataaaaat actactaaaa aggctagttg ctaaaaggta tttggctaga tatattataa
      241 attttcaata tcattataaa ataataatag ataataaaga atattatact tttaagaaaa
      301 aggtactaaa atctagtaaa agcataaaaa taatgcgttc acttaccact acacataaaa
      361 atgtaagtaa agagaagtta gacctattag atgagtatta tagaaattta aaagaatgat
      421 atagtaaaaa tcgaatgtaa ttatttacct taattctttt gtttgtaaaa ctttattgat
      481 atagaattta acatatacat aaattggtaa agggatgtat actatgaaaa ttagtaaatt
      541 accagaagcg gaattaaaag ttatgagata tatttgggaa tcagaaaagg tattaatatc
      601 aagagaaatc accaagtcta tggaacaaaa atatgaatgg aaagatacaa ctatatttac
      661 agtattaaaa agattactaa agaagagagt ttttagaaac ggagaaaata gataagcgta
      721 cacactataa tgtattagta aaagaaaaat aatatcagag attttaaaca aaagaatttt
      781 taaagaatac acataaaaat tcaatattaa gtttaatgtc agcactatgt agtaaggatg
      841 atgaagtaga tggtgatatt ttaaatttat tagaagaata ttttaagaat ttaaaataag
      901 aaaatgacag ctagatacat aataaataaa atgaaggtat gaaattataa ctttttctac
      961 attcattttg tttattattc tgaacatgtt aaaaaaatga aataagtttg tattttattt
     1021 atataaattt taatagaata tgtacagtat atagtagata gaatgtataa aaatagaatt
     1081 taaataagta gctattattg cagtgaaata aatgtttcta agtatatttg ggtggaagaa
     1141 tggtggaaga aaa
//
LOCUS       DAGQWR010000305         1146 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.312, whole genome shotgun sequence.
ACCESSION   DAGQWR010000305 DAGQWR010000000
VERSION     DAGQWR010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.312"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..>1146
                     /locus_tag="MIW95_003945"
     rRNA            <1..>1146
                     /locus_tag="MIW95_003945"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          293 a          253 c          352 g          248 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtactag gtgtcggggg ttaccccctt
      601 cggtgccgca gctaacgcat taagtactcc gcctgggaag tacgctcgca agagtgaaac
      661 tcaaaggaat tgacggggac ccgcacaagt agcggagcat gtggtttaat tcgaagcaac
      721 gcgaagaacc ttacctaagc ttgacatccc aatgacatct ccttaatcgg agagttccct
      781 tcggggacat tggtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg
      841 gttaagtccc gcaacgagcg caacccttgt ctttagttgc catcattaag ttgggcactc
      901 tagagagact gccagggata acctggagga aggtggggat gacgtcaaat catcatgccc
      961 cttatgctta gggctacaca cgtgctacaa tgggtagtac agagggttgc caagccgtaa
     1021 ggtggagcta atcccttaaa gctactctca gttcggattg taggctgaaa ctcgcctaca
     1081 tgaagctgga gttactagta atcgcagatc agaatgctgc ggtgaatgcg ttcccgggtc
     1141 ttgtac
//
LOCUS       DAGQWR010000306         1120 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.313, whole genome shotgun sequence.
ACCESSION   DAGQWR010000306 DAGQWR010000000
VERSION     DAGQWR010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.313"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(2..73)
                     /locus_tag="MIW95_003946"
     tRNA            complement(2..73)
                     /locus_tag="MIW95_003946"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Asn,seq:gtt)
     gene            complement(80..153)
                     /locus_tag="MIW95_003947"
     tRNA            complement(80..153)
                     /locus_tag="MIW95_003947"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Cys,seq:gca)
     gene            complement(161..236)
                     /locus_tag="MIW95_003948"
     tRNA            complement(161..236)
                     /locus_tag="MIW95_003948"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(201..203),aa:Lys,seq:ttt)
     gene            complement(245..321)
                     /locus_tag="MIW95_003949"
     tRNA            complement(245..321)
                     /locus_tag="MIW95_003949"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(285..287),aa:His,seq:gtg)
     gene            complement(329..405)
                     /locus_tag="MIW95_003950"
     tRNA            complement(329..405)
                     /locus_tag="MIW95_003950"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(369..371),aa:Pro,seq:tgg)
     gene            complement(429..505)
                     /locus_tag="MIW95_003951"
     tRNA            complement(429..505)
                     /locus_tag="MIW95_003951"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(469..471),aa:Ile,seq:cat)
     gene            complement(520..593)
                     /locus_tag="MIW95_003952"
     tRNA            complement(520..593)
                     /locus_tag="MIW95_003952"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(557..559),aa:Met,seq:cat)
     gene            complement(600..675)
                     /locus_tag="MIW95_003953"
     tRNA            complement(600..675)
                     /locus_tag="MIW95_003953"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(640..642),aa:Phe,seq:gaa)
     gene            complement(679..767)
                     /locus_tag="MIW95_003954"
     tRNA            complement(679..767)
                     /locus_tag="MIW95_003954"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(731..733),aa:Ser,seq:tga)
     gene            complement(856..931)
                     /locus_tag="MIW95_003955"
     tRNA            complement(856..931)
                     /locus_tag="MIW95_003955"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(896..898),aa:Gln,seq:ttg)
     gene            complement(939..1015)
                     /locus_tag="MIW95_003956"
     tRNA            complement(939..1015)
                     /locus_tag="MIW95_003956"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(979..981),aa:Arg,seq:tct)
BASE COUNT          299 a          302 c          256 g          263 t
ORIGIN      
        1 gctccaaggg tggggctcga acccacagcc taccggttaa cagccggttg ctccaccgtt
       61 gagctacctt ggaatatttt ggaggcgaca cccagattcg aactggggat caaggagttg
      121 cagtccactg ccttaccact tggctatgtc gccacctttt tggtgatcta tccgcgactc
      181 gaacgcggga caccctgatt aaaagtcagg tgctctaccg actgagctaa tagaccatat
      241 gaattggggt ggataatgag agtcgaactc acgacctcca gagccacaat ctggcgctct
      301 aaccaactga gctataccca ccttatattg gtcgaggtga agggactcga acccccggcc
      361 ccatggtccc aaaccatgtg cgctaccaaa ctgcgctaca cctcgaattt atattaatta
      421 atttaatgtg gtgggccatc agggactcga accccagacc taccggttat gagccgggcg
      481 ctctaaccaa ctgagctaat ggcccacatt aaattttagt agcggtaata ggagtcgaac
      541 ctatgacctt tcgggtatga accgaacgct ctagccaact aagctatacc gccatatttt
      601 ggtgcccaga ggcggaatcg aaccaccgac acggggattt tcagtcccct gctctaccga
      661 ctgagctatc tgggcatttg gcggagaggg tgggattcga acccacggcc cctttcgaag
      721 tcactggttt tcaagaccag ctccttaaac cactcggaca cctctccaca ttcggatact
      781 taaacttatt ctaaaattga acctaactat atttaaaata aaaatagttc aaatatagct
      841 tagtaataca tcatttggct ggggatgcag gactcgaacc tacgcataca tgagtcaaag
      901 tcatgtgcct taccgacttg gctaatcccc aatatatgtg gtgagcgcac agggattcga
      961 accccggaca cacgccttag aagggcgttg ctctatccag ctgagctatg cacccacaat
     1021 ttatatatgt atatcaaaca aaaatggtgc ggatgaaggg agtcgaaccc ccacgccaat
     1081 ggcgctagat cctaagtcta gtgcgtctgc caattccgcc
//
LOCUS       DAGQWR010000307         1069 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.314, whole genome shotgun sequence.
ACCESSION   DAGQWR010000307 DAGQWR010000000
VERSION     DAGQWR010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.314"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            53..958
                     /locus_tag="MIW95_003957"
     CDS             53..958
                     /locus_tag="MIW95_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HBY3153201.1"
                     /translation="MESKEKSILRATHFGIIKIGEKDLNCAVLENGSRIINKSTVFKA
                     FGRTKRGRKKDEIRVPSKPNLPSFIDAKNLSDYISDELEDMLINTIKYKTKNGRIVEG
                     YPAEIIPLICDVYLQARLDNKLAVQQEGLAIASEILVRSLSKIGIVALVDEATGYQYD
                     RDRDELQKILSMYISKEFLPWTKRFPDEFYRQMFRLKNWTYPRPSSKRPGIVGTYTNK
                     YVYDLLPPGVKEELQRVNPTVKPGQRKHKHHQFLTEDIGNDHLKNHLLKVITLMQASK
                     DWKDFNVLFNRAFNLPVQLEIEYDE"
BASE COUNT          440 a          129 c          172 g          328 t
ORIGIN      
        1 agatactaag taatatctta cttattaaaa taaaataaag gatggtgtta aattggaatc
       61 aaaagaaaaa agtatattga gagcaacaca ttttggtatt ataaaaatag gtgaaaaaga
      121 tttaaattgt gctgtactag aaaatggttc aagaatcata aataaaagta cagtattcaa
      181 agcttttgga agaacaaagc gtggtagaaa aaaagatgaa attcgtgtac cttctaagcc
      241 taatctacca agttttattg atgcaaagaa tctatctgat tatatttcag atgaattaga
      301 agatatgctt ataaatacaa taaaatataa gactaaaaac ggaagaattg tggaaggtta
      361 tccagctgaa ataatacctc ttatatgtga tgtatattta caagcaaggc ttgataataa
      421 attagctgtt cagcaagaag gactagctat agcaagcgaa attttagtaa gaagtttatc
      481 caaaataggg attgtagcat tagttgatga agctacagga tatcaatatg atagagatcg
      541 tgatgagctg caaaaaatat tgtctatgta tatttctaaa gagtttttac cttggacaaa
      601 aagatttcct gatgaatttt atagacaaat gttcagatta aaaaactgga cataccctag
      661 accaagttct aagagacctg gaatagttgg aacatacaca aataaatatg tttatgattt
      721 attacctcct ggagttaaag aagaacttca aagagttaac cccacagtaa aacctgggca
      781 aagaaaacat aaacatcatc aatttttaac agaagatata ggtaatgatc atttaaaaaa
      841 tcatttatta aaggttataa ccttaatgca agcatcaaaa gactggaaag atttcaatgt
      901 gctttttaat agagctttca atctacctgt acagctcgaa atcgaatatg atgaataata
      961 gactatagcc tttaacaacg ctttaaatac ttttaattta tattataaaa atagaattaa
     1021 cttatatcat aaaagtactt attagtatag gtacttttat gatatatta
//
LOCUS       DAGQWR010000308          997 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.315, whole genome shotgun sequence.
ACCESSION   DAGQWR010000308 DAGQWR010000000
VERSION     DAGQWR010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.315"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            638..>997
                     /locus_tag="MIW95_003958"
     CDS             638..>997
                     /locus_tag="MIW95_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY3153202.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKE"
BASE COUNT          396 a          156 c           96 g          349 t
ORIGIN      
        1 cagccacata taatatagca atacttatta tacattcgat tgatataaaa ccactctttc
       61 ttattttcaa taataaaaac aacccctaaa taatatttta tatataaaca tttaactata
      121 agttttttca taaatatctt ttgtaagttt tttcattgta atcaaaattt cttcccatac
      181 atatataaat taacacacca ccttcatcct taacagaatt aactatgact gatacttttt
      241 gcttgtaatc aactttatac aaacaattaa ttacatttac ttttttaagt attgtaaaat
      301 tacacctcct atattcatat ttttatagac tctaatgctt tgcattaatc ttttacctca
      361 acaccaaaac atatataaat actatttcct tttatattta ttgacacctc aattccaatt
      421 tatactttat aaatcaataa tttcttcgcc ttgtttcata tatatacaac aaaaacttaa
      481 atttaaattc ccactcttta tgtcatattc cattcaaaag tttgcaaata tatgttttaa
      541 attctaaaaa tatgcatatt attttattag ataacagtca acttggataa gtctccttca
      601 tataatttaa agtgaccgtt gaaaaggggg tgacctattg gcagctctta aatcttttga
      661 aaaaccctta acccctgaag aagagattga gtatttaact aaatttaaaa tagaaaacga
      721 taaaagtgca aaagatacat taattgaaag aaacatgaga ttagtagctt atatagcaaa
      781 gaaatataat aattcaacgg aagatcagga tgatttaata tccataggta caataggatt
      841 aattaaagct atagaaactt ataacataga taagggaact cgattagcaa catatgcttc
      901 tagatgtatt gaaaatgaga ttttaatgaa tattagatct aacaaaaaaa ataaaactca
      961 agtatctcta caagacccca tcggaacaga taaagaa
//
LOCUS       DAGQWR010000309          988 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.316, whole genome shotgun sequence.
ACCESSION   DAGQWR010000309 DAGQWR010000000
VERSION     DAGQWR010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.316"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(258..725)
                     /locus_tag="MIW95_003959"
     CDS             complement(258..725)
                     /locus_tag="MIW95_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY3153203.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDILGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKGIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEIGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          378 a          165 c           67 g          378 t
ORIGIN      
        1 agcttgatag taagtataat tatcttttat aagctaatac ttatattttt ctaaatttaa
       61 atcatccaat aaatattaaa ttctattata atatgtaagg aaaatcatgc actttttaaa
      121 aatccaaata aaattataac aaatattata ttctgtatct tacaaaaaca aaaactgtaa
      181 gtaaatttgt ttccatacaa gtttacttac agcccttact tctatacaaa atatagtttt
      241 actttaatat aatccatcta ttctaaaact gattcaatat attcgacagt tacatccatt
      301 cttttagcta cctcatctat tgaaagacca ctatctaaaa aacgtctaca cacagatgac
      361 atagtttctc caatctcaat aatatgccca tctaaatcat aaaatctaat tgctctttga
      421 ccccacctat gctctatagc tagatgaaca taatctatac ctttcattgt actcaatctt
      481 tcaacaaaag tatcaaaatt atcttcctca aaatataact cagcatcatt tccattgaac
      541 ttaacttcac tgtcactctt attaataaat tctaaccaaa gctttttttc ttgtaatgat
      601 atattacctt ctaatacaac attttctcca aaatcacaga ttacctttaa ccccaaaata
      661 tcataataaa acttttttga tttctcaata tcttcaacta ctattaaaac atttaaaaac
      721 ttcatattac ctccttaata tacacatact ttatttataa tatataatta gaattaaact
      781 ataaaggttt caataaggtc tagtataaag aaaaaaatta taagtcttat tgaaatagtt
      841 aagttaattt tatcatataa tataaatttt aatcttgtaa aaaacagaca tgttcatact
      901 tttaggttta aatctatatg tattcttatt aattttgcta aaactgttat ttttgtagac
      961 tattttagat tactgaaata tctatgtt
//
LOCUS       DAGQWR010000310          975 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.317, whole genome shotgun sequence.
ACCESSION   DAGQWR010000310 DAGQWR010000000
VERSION     DAGQWR010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.317"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     regulatory      64..157
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            240..725
                     /locus_tag="MIW95_003960"
     CDS             240..725
                     /locus_tag="MIW95_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBY3153204.1"
                     /translation="MISELMLKNFGKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          436 a           76 c          140 g          323 t
ORIGIN      
        1 tatatttaaa taatataaaa tttgaggtaa tattattttt taattgttca aattatgtta
       61 ttattcagat ataggcaata ttagataaat ctaatgacgc aaaactatag ggactaaaat
      121 ctttatgtaa taaaggtcat gtcagccagt tgccaaaaag ataataattc tttttgttaa
      181 aagtattttt agagttatat attttaataa cttaatattt ggcaagagga gggcttaaga
      241 tgatatctga attaatgtta aaaaattttg gcaaatctag catcaatttt aaaactaaaa
      301 ttaaattgaa tttaagagaa aaaccagaca taaatagttt gaaattaaaa agtataccag
      361 aaggtaaaat tgttaagtta aaatgtgtag atggaatatg ggcagaagtt gagagtaatt
      421 atgacaaagg ctggttactt tataaatatt tagaaagatt aagtaatgct aaaaacaata
      481 gtaatgatac aataaaaaat agaaaaaaga attacatagg aaatataaga acaaatggat
      541 taagtttaga acttagaaat gatagaacat tagaatctaa agttataact acaatccctg
      601 atggttttaa agttgaagta tgttatagcg ttggaaaatg ggcaagagtt aatatcaata
      661 aaaatgaaaa acgatattct ggatatgtat ataatcaata catagaagag catttaagtt
      721 tataatacga tactagtgag ctttaaatat gttaataaat cacaaaaaag aatcccatta
      781 ataggattct tttttgtaga aacattataa cagaactaaa gagaataaat taactaattt
      841 atttttatta taaagtaatt ataaatggta aatttaattg aaagtagatt taacttatac
      901 tttatacacc aagtatcata aattaatact actttgaaaa attttaaaaa aagtaaaagt
      961 tatgataata aaaat
//
LOCUS       DAGQWR010000311          965 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.318, whole genome shotgun sequence.
ACCESSION   DAGQWR010000311 DAGQWR010000000
VERSION     DAGQWR010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.318"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     regulatory      98..183
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            283..945
                     /gene="zmp1"
                     /locus_tag="MIW95_003961"
     CDS             283..945
                     /gene="zmp1"
                     /locus_tag="MIW95_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBY3153205.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHAYSTTIQQNKDTLSQIVVF
                     PTGNYNKNEANAMVNRLANIDGKYLNVLKQDNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAEAFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
BASE COUNT          401 a          117 c          160 g          287 t
ORIGIN      
        1 tttttgaaga tttatgtaaa aaaataataa ctttttagaa aaaagttcca aagggatata
       61 gacaattaaa aagatataag ctataatata gatataaaat taaatgaggc aaaactaggg
      121 aaacctagtc acgcaaagct ataggggcta aagtttagta aaccatgcca gccagttgcc
      181 gaaaagagta aaaatttctt tttgtatata caaagatact cttgggtatc ttttttattt
      241 tgaaatttat tactcccggc ctaactcaaa ggagggaata atatgagacc aagtaaaaaa
      301 ttattaatag ctataatatc aatattctta ataagttcag ttccagtaag tgcacatgca
      361 tatagtacta ctatacaaca aaataaagac acacttagtc aaatagtagt ttttccaact
      421 ggaaattaca ataaaaacga agctaatgct atggtaaata gattagctaa tatagatgga
      481 aagtatttaa atgtattaaa gcaagataac ttaaaaataa aattattaag tggaaagtta
      541 acagatgaaa aagaatatgc ttatttaaaa ggtgtagttc ctaagggatg ggaaggtact
      601 ggaaaaactt gggatgatgt tccaggctta ggtggaagta cagtagctct tagaatagga
      661 tttagtaaca aaggtaaagg gcatgatgca ataaatttag aattacatga aacagcacat
      721 gcaatagacc atatagtatt aaatgatata tctaaatcag cacaattcaa acaaatattt
      781 gctaaagaag gtcgttcttt aggtaatgta aactacttag gtgtatatcc agaagaattt
      841 tttgcagaag catttgcata ttactattta aatcaagata caaatagtaa gttaaaatca
      901 gcttgtccac aaacttatag ctttttgcaa aacctagcta aatagagctt taataaattt
      961 aaaat
//
LOCUS       DAGQWR010000312          954 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.319, whole genome shotgun sequence.
ACCESSION   DAGQWR010000312 DAGQWR010000000
VERSION     DAGQWR010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.319"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..767)
                     /locus_tag="MIW95_003962"
     CDS             complement(<1..767)
                     /locus_tag="MIW95_003962"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBY3153206.1"
                     /translation="MSRKYSISSMCKLIGVSRSGYYKWLSYSKKSSDRGIKDRIIKDY
                     IIEIHKKYRGTYGRKRICTYLNKILDSPINHKKVYRLMKELGIKSIIRKKVYRRKFKS
                     YEVYDNILNREFRANQPLEKICMDITYIPIGKKFLYMNVAKDLFNGEIVAYEISTKMD
                     TKLVNKTVNQLINMNLAKDCILHTDQGSQYTSRSYSKRLKDNGIIQSMSRRGNCWDNA
                     PIESFFSHFKSELIYLIDTTDPKEMISLINDYIYFYN"
     gene            complement(794..>954)
                     /locus_tag="MIW95_003963"
     CDS             complement(794..>954)
                     /locus_tag="MIW95_003963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153207.1"
                     /translation="KTAFNEETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKL
                     STLLDKSK"
BASE COUNT          304 a          155 c           91 g          404 t
ORIGIN      
        1 ttattataaa aataaatata atcatttatt agactaatca tttcctttgg atctgtagta
       61 tcaattaaat atattaattc agatttaaaa tgactgaaaa aactttctat aggagcatta
      121 tcccagcagt tgccccttcg ggacatagat tgtataatac cattatcttt aagcctttta
      181 gagtaagatc tacttgtata ttgtgaacct tgatctgtat gtaaaataca atctttagct
      241 aaattcatat ttattagttg atttactgtt ttattaacta atttagtatc catttttgta
      301 cttatctcat atgccactat ttcaccatta aataaatctt tggctacatt catgtataag
      361 aattttttac cgataggtat gtatgttata tccatacaaa ttttttctaa tggttgatta
      421 gctctaaatt cacgatttaa tatattatca tatacctcat atgatttaaa cttacgtcta
      481 tatactttct ttctaataat agatttaatt cctagttctt tcatcaacct atatactttc
      541 ttatgattaa tagggctatc aagtatttta tttaaataag tacaaattct ttttctaccg
      601 taagttcctc tatatttttt atgtatttca attatataat ccttaataat tctatctttg
      661 atacccctat cggaagattt tttagaataa cttagccatt tatagtaacc gcttcttgac
      721 acacctatca acttacacat actagaaata ctatacttcc tagacataag tttaattgtt
      781 ttaaatttat cgcttacttt gacttgtcca atagggtact taactttttt aaaaattcat
      841 tctccatgcg taagtacttc aattcttctt caacagattt aaattttcta ttatcatttt
      901 ctagactaat atttttaaat ctgcctctag tttcctcatt aaatgcagtt ttac
//
LOCUS       DAGQWR010000313          908 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.320, whole genome shotgun sequence.
ACCESSION   DAGQWR010000313 DAGQWR010000000
VERSION     DAGQWR010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.320"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..423
                     /locus_tag="MIW95_003964"
     CDS             <1..423
                     /locus_tag="MIW95_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY3153208.1"
                     /translation="TISKKYKMRSVNPLKAWVKEYNLHKEIKETPKGGLFMETRKTTL
                     DERVEIVKDCIEHDFNYNLIAKKYFVSYSQVRRWTLKYKENGIVGLEDRRGKAKKEDE
                     LTQLDEIKLELKIVKAEKHKLQLENAFLKKLKNIERGW"
     gene            417..>908
                     /locus_tag="MIW95_003965"
     CDS             417..>908
                     /locus_tag="MIW95_003965"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY3153209.1"
                     /translation="MVITLSKNENKYKAIQSLFNEKKASLMQLCEIAKVNRSGYYKWL
                     NRDKSNLELENIKLATLITNIYEEKKGVFCSLRMTLQLNREYKLNVNHKRVYRLMRAV
                     GLRSICRKKKFSYVKCTPEVIAENVLNRKFSADKTSQKWLTDVTEFKLTNGTKAYLSA
                     ILDL"
BASE COUNT          391 a          101 c          164 g          252 t
ORIGIN      
        1 actatctcaa aaaaatataa aatgaggtct gttaatccac taaaagcttg ggttaaggag
       61 tataatcttc ataaggagat aaaagaaacc ccgaagggag gactctttat ggagactaga
      121 aaaacaacat tagatgaaag ggttgaaatt gttaaagatt gtattgagca tgattttaat
      181 tacaatttaa tagcaaaaaa atactttgtt tcatacagcc aagttagaag atggactctt
      241 aaatataaag aaaatggtat agttggttta gaagaccgaa gaggtaaagc aaaaaaagaa
      301 gatgagttaa ctcaattaga tgaaataaag ttggaattga aaattgtaaa agcagaaaaa
      361 cacaaattac aactggagaa tgcattctta aaaaagttaa aaaacataga aagagggtgg
      421 taattacact atccaagaat gaaaataaat ataaagctat tcaatcctta ttcaatgaaa
      481 aaaaagcttc gcttatgcaa ttatgtgaaa tcgctaaagt aaatcgttct ggatactata
      541 aatggctcaa tcgagataaa agtaatttag aacttgaaaa cattaaatta gcaactttga
      601 taactaatat atatgaagaa aagaagggag ttttttgctc tttaaggatg accttgcagt
      661 taaacagaga gtataaactt aatgtcaatc acaagagagt ttacagacta atgagagccg
      721 taggcttaag gtctatttgt agaaaaaaga aatttagtta tgttaaatgt acaccagaag
      781 taattgctga aaatgtatta aatagaaaat ttagtgcaga taaaacttct caaaaatggt
      841 taacagatgt aactgaattt aaattaacca acgggacaaa ggcttattta agtgcaattc
      901 tggattta
//
LOCUS       DAGQWR010000314          894 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.321, whole genome shotgun sequence.
ACCESSION   DAGQWR010000314 DAGQWR010000000
VERSION     DAGQWR010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.321"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            252..818
                     /locus_tag="MIW95_003966"
     CDS             252..818
                     /locus_tag="MIW95_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBY3153210.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT          366 a           68 c          153 g          307 t
ORIGIN      
        1 ataagtaagg catttagaat aaaattttaa aaaaattata attaaaaaaa taatgaaaat
       61 atataatatt agtttagatt ataaaaaata aatatacaaa tacttataaa tacaagttat
      121 atacaagatt tatagtagag taataatctt ataaaataat ttaataaata taaattaaat
      181 tatatgtgta tatattgtac tttaatagtg atataataat aaaaaaattt ttaaagaggg
      241 tgatattaca tatgagaaaa cttgaagaga tattagaata taataaatca tttgtagaga
      301 acaaagaata tgaacaatat gtgacatcaa aacatccaaa taaaaaaata gtagtcttat
      361 cttgtatgga tacaagatta actgagcttt tgcctaaggc tatgaatttg aaaaatggag
      421 atgtgaaact aataaaaaat gcaggagcta ctataatgca tccttttgga agtattatga
      481 gaagtgtttt agttgcaatt tacgaatttg atgtagatga ggttatggtt gtagggcatc
      541 atggatgtgg tatgtgtaat gttgatacag ataagttact tggtaaaatt ttagatagag
      601 gaatatctaa tgatgtaata cttacacttc gtagtgctgg aattgataca aaacaatggt
      661 tacatggatt cgattctgca gaagaatctg taaaagatag tattgattta ataaaagggc
      721 atcctttaat tccagatggt attattgtac atggtctaat tatatcacct gaaactggga
      781 agttagacgt tgttgtaaat ggatatgaag ataagtagag tattgtaatg ttatataatc
      841 aagatactgt tgaatatgat gttgtttcaa aatttatttt gggacaactt tttt
//
LOCUS       DAGQWR010000315          891 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.322, whole genome shotgun sequence.
ACCESSION   DAGQWR010000315 DAGQWR010000000
VERSION     DAGQWR010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.322"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(14..727)
                     /locus_tag="MIW95_003967"
     CDS             complement(14..727)
                     /locus_tag="MIW95_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3153211.1"
                     /translation="MINIIICEGKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLL
                     KKYPNNVDIIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRY
                     LEKPLKYSLFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHT
                     EKGIYTFKNSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSR
                     YKIKLLREKLNSCLVDVII"
BASE COUNT          319 a          119 c           53 g          400 t
ORIGIN      
        1 gcaaaaattt acctcaaatt attacatcta ccaaacaact atttaacttt tctctcaata
       61 attttatctt ataacgactt acaattattg aattttgatt tataaataca atattatttt
      121 ctatatctag catatttacc ttcattaaat ttataaggta gcttttatga catctaaaaa
      181 aattatgttc ttctagattt gattctattt ctttcatact attcttaaaa gtatatattc
      241 ctttttcagt atgtatagtt aaatcatttt ttaaaacttc taaatataaa atatcatcta
      301 cctttatgaa atatatcttg catttacatt ttatagataa aatagcttca tttttgtagc
      361 attcttttaa atatgatgat attgcttctt taaatagaga gtattttaag ggcttctcta
      421 aatatctata aactcctatc tcataacctt tctttatgta tttatttgat gaggatacaa
      481 atataattct tacactttca tcaaattttt tgatttcttt tgaaacatta aatccatttt
      541 catcatttaa gtctatatcc ataaatatga tatcaacatt attaggatac ttttttaata
      601 aatcgcctcc attttcaaac tctttgatat tgtattcaaa attgtaatta taaaaaaagg
      661 tatttatata atcatttaga attaccctct ctgatttttt accttcgcaa ataataatat
      721 taatcatatt acacccctta tataattttt tattttaaat tttataaaat tctatctaac
      781 atttattata tttcaaattt aaagatattt tttaatacaa aatttttgta taataataat
      841 tattatttaa tctaaatata tctatatttt atacatttat acataaatta c
//
LOCUS       DAGQWR010000316          877 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.323, whole genome shotgun sequence.
ACCESSION   DAGQWR010000316 DAGQWR010000000
VERSION     DAGQWR010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.323"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..625
                     /locus_tag="MIW95_003968"
     CDS             <1..625
                     /locus_tag="MIW95_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBY3153212.1"
                     /translation="AGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPNLNNNTSTSE
                     IDISSSADISVIKLANKTEACVEEQIDFVIVVSNAGPENAQNVTLIDNVSDKLKKAVF
                     SLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTCLDRLTNIAEVTSTTPDSNLN
                     NNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDFWC"
BASE COUNT          354 a           99 c          127 g          297 t
ORIGIN      
        1 tgctggagag ataagaaata tcataataag gggaatagta agtcaaacag caataggtac
       61 aattataaat acagcaacag taagttcaac tacacctgac ccaaatctaa ataacaatac
      121 ttcaacatca gaaatagata taagttcatc agcagatatt tctgtaataa aattagcaaa
      181 taaaacagaa gcatgtgtag aagagcaaat agattttgta atagtagtat caaatgcagg
      241 accagaaaat gctcaaaatg ttactttaat agacaatgtt tcagataagc ttaagaaagc
      301 agtattttca cttgatagag gtgtaagttt ccaaccatgg actggaagtc ttaatatagg
      361 tacactacca gctggaactt taagagttat tctattaaga ggaattataa aatcaacatg
      421 tcttgataga ttaacaaata ttgcagaagt tacttcaaca acaccagatt caaaccttaa
      481 caataatata tcaagagtgc aagtagaaat aaaacaatgt tgttgtaatg attgctgttg
      541 taacaactgt agtaattgtt gttgcaatga ttgtggttgt aaagattgtt gctgtaattg
      601 ttgtaaaaat gacttttggt gttgatttta taaaataatt ttgtatacct taaattaaac
      661 tgaaataaaa aataaattca catatgagta aaatatttta taaatattaa taaattttct
      721 atctttgata tggaccacat tcgtaatact agttattaaa tgaagtggtc ctttttaatt
      781 cattaaaatt atttattcta attaaatttt aaatgtatta aataatttta attttttata
      841 aaaaatattc atatattctt tatattaaag tttagat
//
LOCUS       DAGQWR010000317          849 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.324, whole genome shotgun sequence.
ACCESSION   DAGQWR010000317 DAGQWR010000000
VERSION     DAGQWR010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.324"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            239..379
                     /locus_tag="MIW95_003969"
     CDS             239..379
                     /locus_tag="MIW95_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153213.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(376..747)
                     /locus_tag="MIW95_003970"
     CDS             complement(376..747)
                     /locus_tag="MIW95_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY3153214.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
BASE COUNT          310 a           95 c          102 g          342 t
ORIGIN      
        1 aaatagaaaa aacatacaag attctacaat aatagatttt tttgttatta ttacgagttt
       61 tttattgcaa ctgtagaata aaagtattat tatataatta agataattat ctagggaaat
      121 tttggataat caaaatatat tttataaaat caaaatacaa ttacataaac tttattaaaa
      181 atttgttggg aatttctgac tagcattaag aacgtaagtt tgttggggga tggtatatgt
      241 ggaaaagtca aacactactg ttacaaaaaa agaaaaaatt gaagatatag gaatgaaatt
      301 agaacattta ttgagtgaag atattacttt ttatgaatta ttacttgaga ttttgtataa
      361 agaaaaagaa gagaattaat tctcttcttt aaggaatctt tttaaatagg cattatcaat
      421 cattttttct atagcttctc tatcttcttt ttcaagagaa tatatttttt ctaagagctt
      481 ttttgttttt tcacttactg ataagttctc tatgattaat ttatcttctt tgcttatttt
      541 tgtattatca tttacgccta ataaataatc tgttgatgtt tctaatgctt ttgctaatct
      601 aactattatt tcacttttag gttctcttaa attattttca taacgtgaca agctagcctc
      661 agttataccg actttagtag caagttcttt ttgatttata tctaattctt ttcgtagatt
      721 tgctattcta tctcctaaac tttccatatg tacatcactc cttaccgaaa ttataagtta
      781 ttttgataaa aagtaaaata aaattaccaa aaatataaaa aaaaagggtt aaaataataa
      841 aatatattg
//
LOCUS       DAGQWR010000318          815 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.325, whole genome shotgun sequence.
ACCESSION   DAGQWR010000318 DAGQWR010000000
VERSION     DAGQWR010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.325"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            93..713
                     /locus_tag="MIW95_003971"
     CDS             93..713
                     /locus_tag="MIW95_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBY3153215.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
BASE COUNT          295 a           81 c          140 g          299 t
ORIGIN      
        1 tttttaatat tagattcatt ttttgagatt ggtatataat aaacatatag agtatataat
       61 aatagttaaa gttaaaaaag gggaaaagca atatgcagta tatacaagaa ttacttgatt
      121 tgactcaaaa ttattgggtg ttagccacaa taatcggctt attaagtgca tttatagaaa
      181 gctttatacc agccttacca cttgttgcta tagttacagc aaatgctgca atacaaggat
      241 tatttattgg atgtttgtta tcatggattg gctcaggact tgggacaact tcattattct
      301 tattaataag cagatttaat gatagtaagc tttttaataa gttgagaaat agtaaaactg
      361 aaaaagcaat aagttggatg gataaacagg gatttaagtt attatttata gcgtatgcct
      421 gtccatttat gcctggttgt ttagttacta tcgcatcagc ttttggtaaa aaagatttaa
      481 aagattttgt gccagcaatg ttggcaggaa aatttgtaat gtttatagtt ataagttacg
      541 tggcaagtga tatagaaggt tttataacaa gtcctttaaa gatagcatca tttatattat
      601 tggtgttttt gtcatggaag attggaaata gagtaaataa aaatcttgaa aatcataatt
      661 atgatttcca tcataaaaaa catcacaatg ataaagatga taaaatgatt tagcttgtac
      721 gaaataaaac gtatcttaaa atgatttagt actaaattat tttaagatac gttttatttt
      781 ttgtattaag gtacaaaatt tttagttctt ttgta
//
LOCUS       DAGQWR010000319          813 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.326, whole genome shotgun sequence.
ACCESSION   DAGQWR010000319 DAGQWR010000000
VERSION     DAGQWR010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.326"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
BASE COUNT          335 a           60 c           95 g          323 t
ORIGIN      
        1 atttcaataa taaattgttg atataatata taaaaccatt ataatataca ctaaaaataa
       61 gaatgtataa ggtggtaaat ttatataaaa aagaatatga gttgtaaaat attaaataat
      121 attgtactaa ttggaataat tttaacttta ttatagatta ttttaatgcc attagtactg
      181 atggtgtatt ttaaaattgg tataagcatt acaaatacga atatgccaac tgtaatatgt
      241 aatataagta gctatataca tatgtgctgt cccatatatt gtatcattgt ttatattaaa
      301 aagattatgt aaattggtat caatcaataa atcattttca gaagaaatac ctaaaaactt
      361 aaaaaatacc tatatgtgta tttagttaga ttggagattt ttacatttaa ttttatagtg
      421 acaatattat attattgtta tgatgtctat ttatatgcaa ttactataat atcagaactt
      481 atagtatcat ttgtttcatt atcaataagg ttttttactt tgttttgtca attttattaa
      541 aaaaagttat tgatataaaa gataagaagg ataaaactat atgacagatt tcaaacataa
      601 gagtggatat ttatataata gatattttat agttaatttt attagatttt atttaatact
      661 gtaatattaa tatcacaata tattaataat gtattaaaga ttgaattttg tagataacta
      721 taatttattg agagtgataa ataaaaatta tataatatta cagaagaaag agagggagac
      781 aatattatat ccctcttttt tctataataa cta
//
LOCUS       DAGQWR010000320          800 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.327, whole genome shotgun sequence.
ACCESSION   DAGQWR010000320 DAGQWR010000000
VERSION     DAGQWR010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.327"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
BASE COUNT          312 a           82 c           76 g          330 t
ORIGIN      
        1 aatctctcaa atataaaaaa acaataaatt aactcattgg attttttagt ccataatata
       61 attatagatt attcaacaaa caatctatag aaattgtggt attaatgttt aaataaccaa
      121 ctttttctaa aataaccttt ataggaatat atgagttcat aagtaaagat agcgaaataa
      181 gaaaccagtt tggtatgtaa aatctagatt tttaattaaa ttatcaagat tatatattat
      241 agtatcaaaa atagaaaatt aaaattaaat tttttataat aaattgtata cattataaat
      301 tatattaaat ttttaattta agtaatagta tatgtaatct ttagtcatct tcttaccagt
      361 tttttatcca cttttctaat gaagttattt tttcatatgt taattcctct tgaaataatg
      421 atactaaaag actttttata aaattataac ttaaaagttt atgtattttt ttgatatgaa
      481 tttataatac ttatcttctt taattgagat tgtgtatatt taactaaata ttacaacaat
      541 aattctatga aatcaaatca attatttaca tattttttta gaacaataat aagtatatat
      601 tcacaaaatt ttgtgttaat atttttaatt tgtgatataa taaaagcaag gaaacatatt
      661 ttacttaact ttgagtgatg tttccttcaa taaattattt tgaagattta cttttctgga
      721 actttatatt aagttcccag ccactcttag ttgtgaacta tacccctctt aggagacagt
      781 tgaaatatgt aaaactgtct
//
LOCUS       DAGQWR010000321          800 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.328, whole genome shotgun sequence.
ACCESSION   DAGQWR010000321 DAGQWR010000000
VERSION     DAGQWR010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.328"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            125..790
                     /locus_tag="MIW95_003972"
     CDS             125..790
                     /locus_tag="MIW95_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153216.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
BASE COUNT          328 a           79 c          111 g          282 t
ORIGIN      
        1 gtgatttttt tcaaattttt tcttatttac tatatcatat ttatccaatt tatgatatta
       61 tttaaatata atataagttt tataaagtat attaaatata ttataagatg gaggtcgagt
      121 aattttgaaa ggaaacatag tacaatataa ttttgcagat atagaggaag aagtatattc
      181 gttggattat gctatagcat ggaatacaga tgaggaaaac gtaaacataa taccatttac
      241 aaataaattc tgtaaagaat ctatagaatc tttttgccta ggtaaaatta ataactttgt
      301 tgaaatatta aatgaaggtt ttgttgaaaa tcatcattat gtacatttag ataaaatgat
      361 tagtgttcct aagaaaaagg ttaatttagt atatcaacaa gacacacatg gttatcttct
      421 aagagatgat aatgacaacc ttattcctgc aaaaataact tcagagcaat ctaaatctat
      481 atcaagtaag atggagttat tctgtgctgg tgaagaaaaa tgtttaatta atatattact
      541 aaaggctgac ccatcatata ttttagatgt agatagtata aaagacaaaa acattctaaa
      601 tctaggttat gagtctattg acagatataa agaatacaat tttgatgatg ataagatttt
      661 aatatttttt ataaataaaa aacgttattc agtgattatg aaaaaaacaa acaactctga
      721 taacgattta gtttccagaa acaatgctat taaagaatta tttactaata aagcaggaaa
      781 tcttaactaa tattataatt
//
LOCUS       DAGQWR010000322          783 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.329, whole genome shotgun sequence.
ACCESSION   DAGQWR010000322 DAGQWR010000000
VERSION     DAGQWR010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.329"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            352..>783
                     /locus_tag="MIW95_003973"
     CDS             352..>783
                     /locus_tag="MIW95_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY3153217.1"
                     /translation="MNLQSKWVNFLKPTYVSDEIKRKKLQVIIFFSITYGLSYSLGLL
                     AICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFYSSVILFFIIYFFIFI
                     IESLKLISINQFQLMYFMLILISSLCVIIYSYKITSLYIFKN"
BASE COUNT          318 a           54 c           84 g          327 t
ORIGIN      
        1 gttagaatat aaataaaatt atatattatt ttaatattca ctcgaactag aaaataatat
       61 ttgttgtatt tttttgtata taaaaatata ttgtaatatt aaaaaaataa tagtaaactg
      121 attgcataaa aatgattttt atataaattt taaacagaag aataaatttc taacgaaatt
      181 ggatatttct tctgtaaata catattcaaa aaaagaatta taagagttta gtcagcagtc
      241 gtgtaagtaa taaggacttt ataaatatat agaaatataa tgatatgagt ataagtagaa
      301 aaattttatg atttttacaa ttatttttta taaaaaggga ggttatgttt gttgaattta
      361 caatcaaaat gggtaaactt tttaaagcca acatatgtca gtgatgaaat aaaaagaaaa
      421 aaactacaag ttataatatt tttttctata acttatggtt tatcttattc actaggttta
      481 ttagcaatat gttttaatca tttaatagat tcagaaattt tagctcaatt tatgatgata
      541 ttgccattat cttcagtttc tattgcaaaa ttttacacag aaggaagaaa taatgataaa
      601 tataatttct attcatcagt aatattattt tttattattt acttttttat atttattatt
      661 gaatcattaa agttaataag tattaatcaa tttcaattaa tgtattttat gttaatttta
      721 ataagtagtt tatgtgttat tatttattct tataaaataa caagtttata tatttttaaa
      781 aat
//
LOCUS       DAGQWR010000323          752 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.330, whole genome shotgun sequence.
ACCESSION   DAGQWR010000323 DAGQWR010000000
VERSION     DAGQWR010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.330"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..>752
                     /gene="tuf"
                     /locus_tag="MIW95_003974"
     CDS             <1..>752
                     /gene="tuf"
                     /locus_tag="MIW95_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBY3153218.1"
                     /translation="ELLELVEMEVRDLLTEYDFPGDDTPIVRGSALMALEDPKSEWGD
                     KIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEVEL
                     VGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAH
                     TKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTME
                     VDLINSIVVEEGLRFSIREGGRTVASGVVATI"
BASE COUNT          310 a           94 c          187 g          161 t
ORIGIN      
        1 gagttattag agttagtaga gatggaagta agagatttat taacagaata tgatttccca
       61 ggagatgaca ctccaatagt aagaggttca gcattaatgg cattagaaga tccaaagagc
      121 gagtggggag ataagatagt agaattattc gagcaaatag atgagtatat accagctcca
      181 gagagagata cagataagcc attcttaatg ccagtagagg atgtattctc aatcacagga
      241 agaggaacag ttgcaacagg aagagtggaa agaggagtac taaaagtaca agacgaagta
      301 gaattagtag gattaacaga agcaccaaga aaagtagtag taacaggagt agagatgttc
      361 agaaaattat tagaccaagc acaagcaggg gataatatag gagcattatt aagaggagta
      421 caaagaaacg agatagaaag aggacaagta ctagcaaaga ctggatcagt aaaggcacac
      481 acaaagttta cagcagaagt atatgtactt aaaaaagaag agggtggaag acatacacca
      541 ttctttgatg gatatagacc acaattctat ttcagaacaa cagacgtaac aggagcttgt
      601 aagttaccag aaggaataga gatggtaatg cctggagata acgtaacaat ggaagtagac
      661 ttaataaact caatagttgt agaagaggga ttaagattct caataagaga aggtggaaga
      721 acagtagctt caggagttgt tgctacaata at
//
LOCUS       DAGQWR010000324          723 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.331, whole genome shotgun sequence.
ACCESSION   DAGQWR010000324 DAGQWR010000000
VERSION     DAGQWR010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.331"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..>723
                     /locus_tag="MIW95_003975"
     CDS             <1..>723
                     /locus_tag="MIW95_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBY3153219.1"
                     /translation="KNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGD
                     STTMYPSQNISDEIKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRT
                     VPYISKVSGVPIVDLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEV
                     SLGPEMKSTGEVLGVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAK
                     DMKELGYTFVATEGTAKSLRENG"
BASE COUNT          292 a           75 c          154 g          202 t
ORIGIN      
        1 aaaaatccag tacttataga taaatatcta actggtagag aaatagaagt agatgctata
       61 tgtgataagg aagatatatt gatacctggt ataatggaac atttagaaag agctggagtt
      121 cactctggag atagtacaac aatgtatcca agtcaaaata tatctgatga aataaaagaa
      181 aagatagtag aatacactaa aaaaatagct ttagacctta atgtgcttgg tatggttaat
      241 atacaattta tagaatttca aaatgaatta tatataattg aggttaaccc tagagctagt
      301 agaacagttc catatataag taaagtaagt ggagtaccaa tagttgactt ggctactaag
      361 tgtatgttag gagctaaatt aaaggattta ggatatggta caggagttta caaagagcca
      421 aaattagtat cagtaaaagt accagtattc tcaatgtcta aattatctaa agttgaagta
      481 agtctaggac ctgaaatgaa gtctacagga gaagtcctag gtgtaggaga aaatttagaa
      541 gaagccttat ataaagggtt cttggcagca ggtagacata tgtctgatga aagaggagta
      601 gtacttgcta ctgtaaataa tcatgataaa gatgagttta tagagatagc aaaagatatg
      661 aaagaattag gatatacttt tgttgctaca gaaggaacag cgaagagttt aagagaaaat
      721 gga
//
LOCUS       DAGQWR010000325          716 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.332, whole genome shotgun sequence.
ACCESSION   DAGQWR010000325 DAGQWR010000000
VERSION     DAGQWR010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.332"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            206..583
                     /locus_tag="MIW95_003976"
     CDS             206..583
                     /locus_tag="MIW95_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY3153220.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
BASE COUNT          300 a           66 c          110 g          240 t
ORIGIN      
        1 taaataacat tctacactat tgtaattaaa tatgtaaaaa gtaaaatatt taaatagata
       61 tattttaaaa tctaactttt aatattgtaa aaaaattaga aatttaacaa aaagtacaaa
      121 aattcacatt tttttcataa agtgtttgac taaaaaattc acatgttgta gaattaaggt
      181 agcaggagtt ttgagggagg tgtaaataat gataagaaaa ataccacaag cagaactaaa
      241 agtaatgaaa ttcatttgga aagtagatgt tacagtgaca tcaaaagatg ttattgaagc
      301 tatggaacaa aaatatggct ggaaacaaac tactacactg acacttttat ctaggctggt
      361 aaaaagagga ttcttagatg ctcaaaaaat agacagatat acacattaca caataagagt
      421 aaaacacaaa gattatttaa gttttgagac aaaggatttc ctaagtaata tacatgatga
      481 ctctttgaga agcttaattt cagcattaca tgatgatgaa aatataggta aagataagct
      541 agattttcta gaaagttatt ttgataactt aaaggatgaa taattatatg ctatatgttt
      601 ttgaaaatct tataaagaca actattgtat gtagtcttgg tatttgtcta ttattatttt
      661 tgaaaagata tttatttaag aagtttagta agagattcaa ttattatatt tggttg
//
LOCUS       DAGQWR010000326          687 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.333, whole genome shotgun sequence.
ACCESSION   DAGQWR010000326 DAGQWR010000000
VERSION     DAGQWR010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.333"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
BASE COUNT          284 a           52 c           96 g          255 t
ORIGIN      
        1 tacaaaaata tatttgagca aataagtaaa gtaataaaaa aatcaagatt tcatatctaa
       61 aaactattct gaacattaat ttaatttata ttttcatagg aaatatttta tataatacac
      121 ttaacaaata taatttaaat ttttctaatt atttactgtc gattaataga tttttttctt
      181 gcatataagt gcatatttat ggtacgattt acatggtaaa ataaaaaagg ggaggtgaaa
      241 aataaatatt cgtgaaaaag ttttcagttt gaggtatttt attaataata tattttgagg
      301 tattttataa ataatatatt ataaataaag agtataacct caataaatag tatcaaagcg
      361 aatatatggg taattacttt aaaataacag tttttatgtt tagattcaca gtgtttatta
      421 taaactaatg tgtaagaaat aggtctaatc tgtgaataga tagtagttta tttatttaag
      481 ttgagaattg gttgagtgaa gtaatatata aatttaaaaa gtaaataaaa acattatttg
      541 aacaaaatat atgttgcttt attcgagact cctttaaaaa agcactaaaa tgtaattaca
      601 gaagttgcta caaaaggggg attttagttt gtaaaatact atcacgtaaa aaataagaat
      661 tgcatatggc gaaggttttt tcatggt
//
LOCUS       DAGQWR010000327          676 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.334, whole genome shotgun sequence.
ACCESSION   DAGQWR010000327 DAGQWR010000000
VERSION     DAGQWR010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.334"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            1..660
                     /locus_tag="MIW95_003977"
     CDS             1..660
                     /locus_tag="MIW95_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBY3153221.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISIFVLIHIILKIKIRKI
                     R"
BASE COUNT          244 a           59 c           87 g          286 t
ORIGIN      
        1 atgttagatg catttataac aattgtttct tctattatag aaatatggtc atgtaaaaaa
       61 gtttttgatt atactagtca gtcaaagacc gacttaaaaa aactaaatat gtattttctc
      121 ttttctgttt ttcttattat ttcttcttta tatataaata taggagctaa tagtagaatc
      181 ttgatatgta tatttgtaat gttcgttttc tataagtcta actataaggt taatatatat
      241 aaatgtctgg taatctcatt tttttattgg ttaatattaa taggtataaa tggattaagt
      301 atgttattag tagcaacagt aagcaatttg gataatatga atgttttact tagtggcagt
      361 ttatatagat atgaagcaat attcttggga aagataatgt taggtatatt actttatgtt
      421 tataaggctt cttttaaaaa ttttaaaata agtaaaaaag agatatacat atgtattcct
      481 atgatagcaa atttattgtc attttttgta ctttatgatt atattattaa tcttgtaaaa
      541 atctctcctg aaagaagaac tgctttttta gtaatagttt tactattaat attatctaac
      601 atttcaatct ttgtattaat acatataata cttaagatta aaattaggaa aataagataa
      661 attttaatac gattca
//
LOCUS       DAGQWR010000328          653 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.335, whole genome shotgun sequence.
ACCESSION   DAGQWR010000328 DAGQWR010000000
VERSION     DAGQWR010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.335"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            346..>653
                     /locus_tag="MIW95_003978"
     CDS             346..>653
                     /locus_tag="MIW95_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY3153222.1"
                     /translation="MNLKLIWINILNPVFIKNEIKRKKIQIIIFFFINFGLTYSLGLL
                     LYYNKFIDPENFASFMMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVL
                     "
BASE COUNT          257 a           65 c           53 g          278 t
ORIGIN      
        1 aatattaaaa ctcaataata aatttatatt cataagttta atttcttcat taattttatc
       61 aactatgcct ctactgccta ttaatttaat gcaataaata ataaatcttt tacaaaaaga
      121 taataaaaat atagaaaaat attttttatt gtccttttgc ttatggtaaa ccacattctg
      181 cctgcaatat aactaaatat atgggtatat agtaaaatta attttattat ttatatataa
      241 ttttataagt tacttttcaa taatattgac tgtcatgaat ttataatatt caataaaata
      301 ttttaattta gttaagaatc tatattgtaa aggagtatta aattaatgaa cttaaaatta
      361 atatggataa atattctaaa cccagtattt attaaaaatg aaataaaaag aaaaaaaata
      421 caaattatta tctttttctt tataaacttt ggtttaactt attcattagg tttactgtta
      481 tattataata aatttattga tccagaaaac tttgcatcat tcatgatgat attacctcta
      541 tcttcagttg ctattgcaaa gttttatact gaaggattaa ataatgatat gtatgaattt
      601 tattcactaa tcatattatt ttttcttatt tattcattat taattgttct taa
//
LOCUS       DAGQWR010000329          646 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.336, whole genome shotgun sequence.
ACCESSION   DAGQWR010000329 DAGQWR010000000
VERSION     DAGQWR010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.336"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..>646
                     /locus_tag="MIW95_003979"
     CDS             <1..>646
                     /locus_tag="MIW95_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBY3153223.1"
                     /translation="CKRSEELGKNTIFNDIKGLQDAYETNLSDISNEISDFRNAYLTF
                     SECNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTFVQNTLSSIKENIYKITSHIN
                     HNEKMQSNTSSLAVVARLISTEWICSQNNDSIADTLFNRYKLLCIWLNKKYGFDYDYK
                     DIKAKFTPKIPHNDLVVANILSQLGDKLSTETGLSQPSFIDNPHAEMEKAKKEQE"
BASE COUNT          278 a           70 c          103 g          195 t
ORIGIN      
        1 tgtaaaagaa gtgaagaact aggaaaaaat acaatattca atgatataaa aggattacaa
       61 gatgcttatg aaactaattt aagtgatata agtaatgaaa ttagtgattt tagaaatgct
      121 tatttaacct tctctgaatg taatattaaa gaagatgatt tgccaaggat gaaagaactt
      181 ggaatacttc aagttaatgg agatggaaaa atagaatggc ttattaaaga catgaatgat
      241 acatttgttc aaaatacact atcttcaata aaagaaaaca tatataaaat tacatctcat
      301 ataaatcaca atgaaaaaat gcaaagtaat acatcaagtt tagcagttgt agcaagatta
      361 atcagtacag aatggatttg tagccagaat aatgatagta ttgcagatac tttatttaat
      421 aggtacaaat tattgtgcat ttggcttaat aaaaaatatg gttttgacta tgattataaa
      481 gatattaagg ctaaatttac ccctaagata ccacataatg atttagttgt agcaaatata
      541 ttaagtcaac ttggagataa attatctact gaaaccggat tatctcaacc aagttttatt
      601 gataatcctc atgcagaaat ggaaaaggct aaaaaagaac aggaaa
//
LOCUS       DAGQWR010000330          623 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.337, whole genome shotgun sequence.
ACCESSION   DAGQWR010000330 DAGQWR010000000
VERSION     DAGQWR010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.337"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
BASE COUNT          243 a           69 c          120 g          191 t
ORIGIN      
        1 tgtatatttt atgttgcaga tacaaatata gtaaaggaga gtacattggt acaactgaga
       61 tacatagctt attaagtttt tctgtcttat gagaattata ttataaaata ttcataaata
      121 ttttgagttt aaattaaata attattttat gcttagatta attgatattt aattaattta
      181 ataacaaaaa cagtgaaaga gtaaataata ttatttacag ggggtaaagc aaagtatgtt
      241 aaataaattg tacttttggc cccgtacata gagaaatcta tgtaggacac aactttaaaa
      301 ccagtaaatc ctaaagcctt gcaactacaa taaaagatag aaagaaactt ttatgaatgt
      361 ggtgaaaacc agaaacaata gtgaggatgg tatatggtga gagcctaagt acttaaaaat
      421 ggacatttgg tcgccaagcc tagaaatagg aaggtcaaac gactatctcg agagagagta
      481 cactataagc gattgatagt ggaaacgggt tgctcttagc aggtaatgct gaagatgaag
      541 aaatagtcta cgctcatatg aaagtatgag gtcactagat ttgaagtgac atctataaag
      601 tagcgtttat aggtaaatgt tta
//
LOCUS       DAGQWR010000331          603 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.338, whole genome shotgun sequence.
ACCESSION   DAGQWR010000331 DAGQWR010000000
VERSION     DAGQWR010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.338"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..>603
                     /locus_tag="MIW95_003980"
     CDS             <1..>603
                     /locus_tag="MIW95_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBY3153224.1"
                     /translation="STIHQMLEAELDDHLGYDRYDNKNKNTKNSRNRYRNKNVKSDFG
                     EITLNIPRDRNGDFEPKVIRNYENDISGIENQVIGMYSKGMSTRDISSHLKSIYGIDI
                     SHSLIAKITDRVLPLAHEWQSRALDMVYPIVFMGAIHYKVRSEGRIINKAAYIAIGVN
                     LEGITEVLGIWIGENESSKYWLKVLTDVKNRGVKDILITSV"
BASE COUNT          242 a           68 c          118 g          175 t
ORIGIN      
        1 agtacaattc atcaaatgtt agaagcggag cttgacgacc atttaggtta tgatagatat
       61 gacaataaga ataaaaatac taaaaactct agaaatagat atagaaataa aaatgttaaa
      121 tcagattttg gagaaataac tttaaatatt ccaagagatc gaaatggaga ttttgaacca
      181 aaggtaatta gaaattatga aaatgatata tcaggaatag agaaccaagt gattggaatg
      241 tactcaaaag gtatgtctac tcgagatata tcaagccacc ttaagagtat ttacggaatt
      301 gatatatctc attctctaat agctaaaatt actgatagag ttctaccttt agctcatgaa
      361 tggcaaagca gagctcttga tatggtttat ccaattgtat ttatgggtgc tatacattat
      421 aaagttagaa gtgaaggtag aataataaac aaagctgcat atatagccat aggtgttaac
      481 ttagaaggta taacggaggt cttaggaata tggattggag aaaatgaaag ttcaaagtat
      541 tggctaaagg tgcttacaga tgtcaaaaac agaggagtta aggatatatt aataacttct
      601 gta
//
LOCUS       DAGQWR010000332          557 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.339, whole genome shotgun sequence.
ACCESSION   DAGQWR010000332 DAGQWR010000000
VERSION     DAGQWR010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.339"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
BASE COUNT          248 a           29 c           67 g          213 t
ORIGIN      
        1 gtttatttat ttaataaata tttcaaataa aataatgttt aaataagaaa cataaataat
       61 aatattatta ttgttcttaa agacaaaaaa tcttagctta aaaatttgta tcatataaaa
      121 tccctacata ttatggaagt aatgatacat tatatgatat acacaataaa gtataaaagt
      181 atttacatgt tttttctata tattttataa gttttaggta tggaaaatta ctaaataaaa
      241 attagattaa tgattatgat agtatagata aagtttattc tttttattaa tagaaattgt
      301 tgtataaaaa taataaaagt gaggagagtg atatttaaat aaaaaaattt taataagtat
      361 tggtaatata gtttttaggg atgattattt tatgtaaaaa gttgttattt gtaaagataa
      421 atatagaaaa tattataagt attgatttag tgataatcta aaaggtgaga attgtaataa
      481 aaagagaaca tatccttcaa atggatgtaa acttataaat aacattatta caagagattt
      541 attatacata tactgca
//
LOCUS       DAGQWR010000333          552 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.340, whole genome shotgun sequence.
ACCESSION   DAGQWR010000333 DAGQWR010000000
VERSION     DAGQWR010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.340"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..519)
                     /locus_tag="MIW95_003981"
     CDS             complement(<1..519)
                     /locus_tag="MIW95_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153225.1"
                     /translation="MSNQIKDFDITNDMFQESIPITTKTILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLVKAANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKK"
BASE COUNT          179 a           79 c           45 g          249 t
ORIGIN      
        1 ttttttaaaa tcttcttcaa ttttattttt attactttta ttaatgttaa cagaatctct
       61 tttaaattta tcaatatatt tttctatttc attaatatta tctttgctta caactatacc
      121 accttttaaa ttaaaaactt ttttgaaatt cttttcagaa aatccttgaa taaaataact
      181 ttgtccatct ccattaatac tatcttgagt atcaaatact tcaatttctt tgttaggtat
      241 tggtatatca aaattccaag attcctttag cattgaaata ttagctgctt ttactaaaaa
      301 aagattatat aggatacttc ctaataacaa aacacctatt aatagtagtg tgattaatac
      361 aatttttctc tttttcattt ttcgttcaca accttttgaa aaaacatgat ttaattcatc
      421 atttgaaggc atgtcttcaa gaatagtttt tgttgtgata ggaatgcttt cttgaaacat
      481 atcatttgta atatcaaaat cttttatttg attactcaat atccatttct tctaatattt
      541 cttttgattt tt
//
LOCUS       DAGQWR010000334          540 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.341, whole genome shotgun sequence.
ACCESSION   DAGQWR010000334 DAGQWR010000000
VERSION     DAGQWR010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.341"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            165..470
                     /locus_tag="MIW95_003982"
     CDS             165..470
                     /locus_tag="MIW95_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153226.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
BASE COUNT          222 a           50 c           72 g          196 t
ORIGIN      
        1 tatatgaaaa ttagaattat tttatcacaa aaaaattaag ttaaataaat atgaaattac
       61 ttccaaatat acttccaaaa acatgtaatc gcatatataa tataattata aaaataataa
      121 atatttttat agtagtagtt aattattaat aaggaggggt caaaatgcct agcatgatta
      181 tggaagttgt aggagtacta tttatagcta ttggattaat cttgacattc attgaaataa
      241 aaggaaattt cccttctttt tatggttttg gtcaaaattc tatagggaaa aatgctgtag
      301 gtattatagc agcaggaatt gctttaattt tctttagaaa tggcatagat agcatagatt
      361 ctatagctac ttatggtgta tttttagcaa tagaacttgt tgtaatagtt gcatatttct
      421 taataagaaa aaaatttact aaagaagata aacatttaag aactaattaa ctaaattatc
      481 tactataaaa ttggaaaatt aaaaacaaat aaaagtttgt ttttaattat ttaaaatccc
//
LOCUS       DAGQWR010000335          517 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.342, whole genome shotgun sequence.
ACCESSION   DAGQWR010000335 DAGQWR010000000
VERSION     DAGQWR010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.342"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..380
                     /locus_tag="MIW95_003983"
     CDS             <1..380
                     /locus_tag="MIW95_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3153227.1"
                     /translation="IKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASII
                     IGITLFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGN
                     LNISLKKTNAKLVPKINQKKEVL"
     gene            381..>517
                     /locus_tag="MIW95_003984"
     CDS             381..>517
                     /locus_tag="MIW95_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3153228.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KS"
BASE COUNT          187 a           73 c           79 g          178 t
ORIGIN      
        1 ctataaaaat actaggactt atgatttcaa acggacttat agctttatct ggtagtctta
       61 tggctcagtt cttgggattc tcagatgtaa atatgggtat aggaacttta gttcttggaa
      121 ttgcctctat cataattggt attacactat ttaagaaatt tacttttatc aaagatacta
      181 ctgcaataat tgttggctct tttatatacc aattcactat atactttgca atgagtttag
      241 gaatgttatc aactgactta aaactaataa ctgccatagt tataatagct ttcctagcta
      301 ctggtaactt aaatatttca ctaaaaaaaa caaatgcaaa gctagtacca aaaataaatc
      361 agaaaaaaga ggtgttgtag atgttacaaa ttaaaaatct ttcaaagagt ttccctaatc
      421 catatggtga acctaatact atctttgaaa atttatcaat cgatatagaa gatggtgaat
      481 ttgtaagtat cataggtagt aatggaactg gtaaatc
//
LOCUS       DAGQWR010000336          454 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.343, whole genome shotgun sequence.
ACCESSION   DAGQWR010000336 DAGQWR010000000
VERSION     DAGQWR010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.343"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..372)
                     /locus_tag="MIW95_003985"
     rRNA            complement(<1..372)
                     /locus_tag="MIW95_003985"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          120 a          109 c           73 g          152 t
ORIGIN      
        1 aggactatta ccttctgtgg tgggtctttc caaacctctt caattacgat acctcttcgt
       61 tatgatatgt ccgcaacccc aataaagaaa actttattgg tttgggctaa tccgcgttcg
      121 ctcgccgcta cttacggaat cgaatttctt tctcttcctt caggtactta gatgtttcag
      181 ttccctgagt tcccctcatt aagctatgta ttcacttaat gatacttaga cattactcta
      241 agtgagtttc ctcattcgga aatcttcgga tcaaagttta cgtgcaactc cccgaagctt
      301 atcgcagctt atcacgtcct tcatcggctc ctagtgccaa ggcatccgcc ctgcaccctt
      361 aataacttga ccagttaaaa aggtttgata gattagaagc tatcaacttc gataatttta
      421 aaaagttatc agctttatat attacaaatt agat
//
LOCUS       DAGQWR010000337          454 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.344, whole genome shotgun sequence.
ACCESSION   DAGQWR010000337 DAGQWR010000000
VERSION     DAGQWR010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.344"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
BASE COUNT          172 a           68 c           50 g          164 t
ORIGIN      
        1 cccagtgcca taatcgataa aattaaactt tgcgtcatta cagatataat acctgacata
       61 aataaaacac ccctttgaaa atattttaaa tggtttttta aatttggttt taaaaaatga
      121 ttttgatata aaaaaaccac acagtatata taactgtatg gcaaaaattc gaataagcca
      181 cacagtataa tccatgtggc tctatattat gatgataaaa taaaaacttt aacaacaatt
      241 actatagcca acatagatac aatccttaaa aatcataatc ctaaggggct tgtatctatg
      301 ttatgacaat ttgtcttaac ttaggtacaa accctaacta aattggctac taaaaaatcg
      361 tacttgtttg tttaataaag tcttcatatt tacctctcct tttaatttaa taattttgaa
      421 aattttgttt tctgttttgt atagtaatta gttt
//
LOCUS       DAGQWR010000338          435 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.345, whole genome shotgun sequence.
ACCESSION   DAGQWR010000338 DAGQWR010000000
VERSION     DAGQWR010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.345"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..177
                     /locus_tag="MIW95_003986"
     CDS             <1..177
                     /locus_tag="MIW95_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBY3153229.1"
                     /translation="TYKTAIDEAQAILDKADATQKEIDDAVTALNTATATFEAGKVPT
                     TIALMLSRILGFMK"
BASE COUNT          203 a           48 c           51 g          133 t
ORIGIN      
        1 acatataaaa cagcaataga tgaagcacaa gcaatattag ataaagcaga tgcaacacaa
       61 aaagaaatag atgatgcagt aacagcatta aacacagcaa cagctacatt tgaagcaggg
      121 aaagtaccta ctacaattgc attaatgtta tcaagaatac taggatttat gaaataagaa
      181 ataaaaagca aattatttaa taaaatttat atatacttta aatagaaaat ttaatttaag
      241 gctgtgagta aaatttttaa aaataaattt atacacagcc tttttatcat gtaaagttaa
      301 ttgatatagt aattttagat aatttagaga tataaaattt aaaatgttag ataaagttca
      361 aagtataacc taatatcaaa acttcacata attttcatat taattatcaa ttaaattcac
      421 acacaagagt tatta
//
LOCUS       DAGQWR010000339          427 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.346, whole genome shotgun sequence.
ACCESSION   DAGQWR010000339 DAGQWR010000000
VERSION     DAGQWR010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.346"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            94..375
                     /locus_tag="MIW95_003987"
     CDS             94..375
                     /locus_tag="MIW95_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153230.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT          187 a           30 c           66 g          144 t
ORIGIN      
        1 aatagtgtta ctttcataat aaatacatat aaagatattt ttaagaataa aatttattat
       61 taaataaaaa ataaaggaga gtgatttaat cgtatggcat caaaacaaac accaagaaac
      121 aatttagaaa agtttttaag aataagagtc ttatcacata caggcgataa aatacatata
      181 aagttacctg taaattttgc aaaaagaatg attgagaata atgctttgga tttatttaat
      241 ggaaaagatg atgtagtaga tggtaaaaag gttactgaaa tattagtcaa agcatttgac
      301 tatgatttaa ctggagaagt agttcacttg gagagaaaaa atggagatat tattaaagtt
      361 tcaattgtac aatagaatta ttttatatga attttatttt tgaatagtta taaataatta
      421 gaaattt
//
LOCUS       DAGQWR010000340          420 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.347, whole genome shotgun sequence.
ACCESSION   DAGQWR010000340 DAGQWR010000000
VERSION     DAGQWR010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.347"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..>420
                     /locus_tag="MIW95_003988"
     rRNA            <1..>420
                     /locus_tag="MIW95_003988"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          106 a           97 c          130 g           87 t
ORIGIN      
        1 cgcctcccaa aaggtaacgg aggcgctcaa aggttctctc agtacggtcg gaaatcgtac
       61 gtagagtgta aaggcaaaag agagcttgat tgcaagacat acaggtcgag caaggatgaa
      121 aatcggactt agtgatccgg tggttctgcg tggaagggcc atcgctcaac ggataaaagc
      181 taccctgggg ataacaggct tatctccccc aagagtccac atcgacgggg aggtttggca
      241 cctcgatgtc ggctcatcac atcctggggc tgtagtaggt cccaagggtt gggctgttcg
      301 cccattaaag tggtacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc
      361 gtcgcaggcg taggaaattt gagaagacct gtccttagta cgagaggacc gggatggacg
//
LOCUS       DAGQWR010000341          401 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.348, whole genome shotgun sequence.
ACCESSION   DAGQWR010000341 DAGQWR010000000
VERSION     DAGQWR010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.348"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..210
                     /locus_tag="MIW95_003989"
     CDS             <1..210
                     /locus_tag="MIW95_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBY3153231.1"
                     /translation="GVELNDLIGEPISYVIPELERRITEALIQDDRIENIDNFEFQNI
                     KGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            223..>401
                     /locus_tag="MIW95_003990"
     CDS             223..>401
                     /locus_tag="MIW95_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBY3153232.1"
                     /translation="MTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTETYIAM
                     DELLDQTFVDTASYY"
BASE COUNT          158 a           40 c           75 g          128 t
ORIGIN      
        1 ggtgttgaat taaatgattt aattggagaa cctatttcat atgtaatacc cgaacttgaa
       61 agaaggataa cagaagcact aattcaagat gataggattg aaaatataga taattttgag
      121 tttcaaaata taaagggtaa agtacaatgt agattttcag ttcatacaaa atatggaaat
      181 ataaaagcag agaaggtggt gagtgtataa ttgtttgagt taatgacatt tgaaaatata
      241 attaaaagaa tgttagatag tgtaccagat acttttgata aaagggaagg ttctataata
      301 tataatgctc ttgctcctgt tgctatagaa cttacagaaa catacattgc tatggatgaa
      361 ttactagacc aaacattcgt agatactgct agttattatt a
//
LOCUS       DAGQWR010000342          395 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.349, whole genome shotgun sequence.
ACCESSION   DAGQWR010000342 DAGQWR010000000
VERSION     DAGQWR010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.349"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            250..>395
                     /locus_tag="MIW95_003991"
     CDS             250..>395
                     /locus_tag="MIW95_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBY3153233.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGI"
BASE COUNT          163 a           29 c           54 g          149 t
ORIGIN      
        1 aatatattaa acaatcaact aaaaaatgaa atattaataa aaaaaattca aattataaat
       61 attaaatgaa tataattaaa acaaatatat aaatttaagt ataacactca cattttaatg
      121 tattgtgaaa tttttgttga actttatgta ccataactta taaaatttat gtcatgttat
      181 atttgaataa gtaataaaat tgtagatgca aattaatcta caaaatttat atgaaatgga
      241 gttgatgcta tgacagaatt aatgattata agtgggttag tactattgat ttgtataact
      301 tctagcaagg tattgtataa atttggagta ccaatattat tgatttttat cttactaggg
      361 atgttatttg ggtctgatgg aatggtagga atcta
//
LOCUS       DAGQWR010000343          375 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.350, whole genome shotgun sequence.
ACCESSION   DAGQWR010000343 DAGQWR010000000
VERSION     DAGQWR010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.350"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            complement(90..206)
                     /gene="rrf"
                     /locus_tag="MIW95_003992"
     rRNA            complement(90..206)
                     /gene="rrf"
                     /locus_tag="MIW95_003992"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(215..288)
                     /locus_tag="MIW95_003993"
     tRNA            complement(215..288)
                     /locus_tag="MIW95_003993"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(254..256),aa:Gly,seq:tcc)
BASE COUNT          135 a           76 c           59 g          105 t
ORIGIN      
        1 atcatttatt gccaaaaaaa tctataaaag acaaataaaa acatataaca attgtaggtt
       61 tattaaattt aatacaacaa aaaaagatta cgtggctacg tcctactctc ccaggcagtt
      121 tcccaccaag taccatcagc actagagagc ttaacttctg tgttcggaat gggaacaggt
      181 gtatcctctc tgctattgta accacataat cttttggagc gggtgaaggg gatcgaaccc
      241 tcacagctgg cttggaaggc cagaactcta ccattgagct acacccgcat taaattttta
      301 taaattagta aaactaattt tttagagtta gcaccctaaa aactacatat atatcctaat
      361 gaatgaacat caaat
//
LOCUS       DAGQWR010000344          371 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.351, whole genome shotgun sequence.
ACCESSION   DAGQWR010000344 DAGQWR010000000
VERSION     DAGQWR010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.351"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..230
                     /locus_tag="MIW95_003994"
     CDS             <1..230
                     /locus_tag="MIW95_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY3153234.1"
                     /translation="LTHLKFFAKRLVKKEKINDTNNEIIDIIKVKYEKDYDCAYKIKT
                     HVEKNYDYYVSQDELLYLTLHIKRVISVLNL"
BASE COUNT          169 a           25 c           44 g          133 t
ORIGIN      
        1 ttttgactca tttaaaattc tttgcaaaac gattagtaaa aaaagaaaaa atcaatgata
       61 caaataatga aattatagat ataattaaag taaagtatga aaaggattat gattgtgcat
      121 ataaaataaa aactcatgta gaaaagaatt atgattacta tgtaagtcaa gatgaactac
      181 tttatttaac attacatatc aaaagagtta tatctgtact gaatttataa taaagagtag
      241 tttaatacag aatgtatagg tttaatatat tatgtttaat tttaatatat tatttaaaat
      301 ttaatataaa ataataattt taaaacatta agattgttac tgataatgca ggcaagactt
      361 atgtttataa a
//
LOCUS       DAGQWR010000345          364 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.352, whole genome shotgun sequence.
ACCESSION   DAGQWR010000345 DAGQWR010000000
VERSION     DAGQWR010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.352"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="MIW95_003995"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="MIW95_003995"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="MIW95_003996"
     tRNA            291..362
                     /locus_tag="MIW95_003996"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DAGQWR010000346          359 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.353, whole genome shotgun sequence.
ACCESSION   DAGQWR010000346 DAGQWR010000000
VERSION     DAGQWR010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.353"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
BASE COUNT          166 a           18 c           53 g          122 t
ORIGIN      
        1 cttaaaaata ataattgaaa cattctgaaa aatataacga aaattaaata ttatctatta
       61 gattcattaa aagttaaaga aaattagcat tttaggattt ttaagatttg tttaaaaaaa
      121 tcaaaattta ttggcatagt aattgcttaa tacatagagt aataatatga ttataaattt
      181 attgattaat tttattatta tttttaaggg gggagagtat ttgtagttat aaagcatatt
      241 gggaataatg aaaaaggtta aaaacatatt actataaagt atagggggta ggttaaaaga
      301 gtaatcataa aataaatata cggataatag tagataagaa taccagaaaa aaatagata
//
LOCUS       DAGQWR010000347          347 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.354, whole genome shotgun sequence.
ACCESSION   DAGQWR010000347 DAGQWR010000000
VERSION     DAGQWR010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.354"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            109..>347
                     /locus_tag="MIW95_003997"
     CDS             109..>347
                     /locus_tag="MIW95_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBY3153235.1"
                     /translation="MSGYLIVNLSNMLGELEEEEVKKILSSFSCPLNKDVEEFLKNKA
                     IEFSKQGLASTHLVLTSYKGKPVIVGYFTLANKYFT"
BASE COUNT          138 a           40 c           61 g          108 t
ORIGIN      
        1 atcagctcta gaaaatgcta aaaataaaca agaaaaagaa gttgtattaa gtaaaaaatg
       61 ttcagaagta ccaaaagata aaataaagga tatattcggg agattttaat gagtggctat
      121 ttaattgtaa acttaagtaa tatgctagga gagctggagg aagaagaagt taaaaaaatt
      181 ctctccagtt tttcttgtcc ccttaataaa gatgtagaag aatttttaaa aaacaaagct
      241 attgaatttt ctaaacaggg tttggctagt acacatttag tgctaacttc ttataaaggc
      301 aagcctgtta tagttggata tttcactcta gctaataagt attttac
//
LOCUS       DAGQWR010000348          332 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.356, whole genome shotgun sequence.
ACCESSION   DAGQWR010000348 DAGQWR010000000
VERSION     DAGQWR010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.356"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     regulatory      94..184
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          141 a           35 c           49 g          107 t
ORIGIN      
        1 taattattgg taactaaatt taaaaaataa aatatttaat tatacacaaa aaagatattt
       61 aaaaaatttg ttatttattg tatacttaag ttaaaactaa ataggcaaat ctagagaaat
      121 ctagtgacgc aaagctatag gggctaaggt ttgtatacac aaactatgtc agccagttgc
      181 caaaaagagt tctaggtata ttgtatacgt aggaagagtc taggatgact gcctttttgg
      241 tggtcatttt gttatggaaa aaatcacatt atgtagtaaa aaacacctat aataacatat
      301 tataccaaat ataatttaaa ataaagataa at
//
LOCUS       DAGQWR010000349          303 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.357, whole genome shotgun sequence.
ACCESSION   DAGQWR010000349 DAGQWR010000000
VERSION     DAGQWR010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.357"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
BASE COUNT          123 a           32 c           28 g          120 t
ORIGIN      
        1 tgaattatca gtataataag tcaaaatatg tatacatgaa tacatatttt taaaagatat
       61 atattattac atacctaaaa aactttcata aaaacaaaaa taaaatatgc ttttagtaac
      121 ttgttaacat aacttataat ttattttata agcattagcc tatatagttt tgtgctacta
      181 aatttctttg attctaacga tttagtgcta ttctacaact aaaataatac aatagagcta
      241 tttattgttt ggtagcattg tttgcaaatt aaacaaatat atatatatga atattatttt
      301 acg
//
LOCUS       DAGQWR010000350          290 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.359, whole genome shotgun sequence.
ACCESSION   DAGQWR010000350 DAGQWR010000000
VERSION     DAGQWR010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.359"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            52..>290
                     /locus_tag="MIW95_003998"
     CDS             52..>290
                     /locus_tag="MIW95_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBY3153236.1"
                     /translation="MSGYSIVNLSNMLEELEEEEVKKILSSFSCPLNKDVEEFLKNKA
                     IEFSKQGLASTHLVLTSYKGKPVIIGYFSLANKYFT"
BASE COUNT          107 a           32 c           51 g          100 t
ORIGIN      
        1 atgttcagaa gtaccaaaag ataaaataaa ggatatattc aggagatttt aatgagtggc
       61 tattcaattg tgaacttaag taatatgcta gaagagctgg aggaagaaga agttaaaaag
      121 attctctcca gtttttcttg tcctcttaat aaagatgtag aagaattttt aaaaaacaaa
      181 gctattgaat tttctaaaca gggtttagct agtacacatt tagtattaac ttcttataaa
      241 ggcaagcctg ttataattgg gtatttttct ttagctaata agtattttac
//
LOCUS       DAGQWR010000351          269 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.361, whole genome shotgun sequence.
ACCESSION   DAGQWR010000351 DAGQWR010000000
VERSION     DAGQWR010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.361"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            130..>269
                     /locus_tag="MIW95_003999"
     CDS             130..>269
                     /locus_tag="MIW95_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153237.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIV"
BASE COUNT          114 a           25 c           38 g           92 t
ORIGIN      
        1 tataattttt ttagaagttt attttttatg taattattta taattttggg taaaaacact
       61 ttgatataat ttcaaaaatt aaaaggttaa aaaacaaagt agaaatttat gttaaataag
      121 gagattaata tgagaagaaa tacaaaatta ttaacaacag ggattctttc aatggcaatc
      181 gtcgcaccta caatggcatt tgctactgaa tctaatgcta tggaaaataa cgctgattta
      241 aatataaact tagagaaaaa aagtatcgt
//
LOCUS       DAGQWR010000352          242 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.denovo.365, whole genome shotgun sequence.
ACCESSION   DAGQWR010000352 DAGQWR010000000
VERSION     DAGQWR010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.denovo.365"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..>242
                     /locus_tag="MIW95_004000"
     CDS             <1..>242
                     /locus_tag="MIW95_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBY3153238.1"
                     /translation="NSLARKIVKFGQYNEELRRYIIGAPLIGQIGKNYSNNYNKLIKG
                     DELLKIACDKIKAVQLDMGGKIVYLECEDKPKLIEF"
BASE COUNT          103 a           22 c           48 g           69 t
ORIGIN      
        1 aactctttag ctaggaaaat agtgaagttt ggacaataca atgaagaact aagaagatat
       61 attattggag cacctttgat agggcaaata ggaaagaatt attcaaataa ttataataaa
      121 ttaatcaagg gtgatgaact tctaaaaatt gcatgtgaca agataaaagc agtacagtta
      181 gatatgggtg ggaaaatagt gtaccttgaa tgtgaagata aacctaaatt aattgaattt
      241 ta
//
LOCUS       DAGQWR010000353        11371 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.guided.134, whole genome shotgun sequence.
ACCESSION   DAGQWR010000353 DAGQWR010000000
VERSION     DAGQWR010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.guided.134"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            71..1948
                     /locus_tag="MIW95_004001"
     CDS             71..1948
                     /locus_tag="MIW95_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY3153239.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            2260..3666
                     /locus_tag="MIW95_004002"
     CDS             2260..3666
                     /locus_tag="MIW95_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBY3153240.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            3694..4542
                     /locus_tag="MIW95_004003"
     CDS             3694..4542
                     /locus_tag="MIW95_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY3153241.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            5203..5895
                     /locus_tag="MIW95_004004"
     CDS             5203..5895
                     /locus_tag="MIW95_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3153242.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            5892..6827
                     /locus_tag="MIW95_004005"
     CDS             5892..6827
                     /locus_tag="MIW95_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3153243.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            6975..7898
                     /locus_tag="MIW95_004006"
     CDS             6975..7898
                     /locus_tag="MIW95_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3153244.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAHAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            7882..8652
                     /locus_tag="MIW95_004007"
     CDS             7882..8652
                     /locus_tag="MIW95_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3153245.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            8662..9444
                     /locus_tag="MIW95_004008"
     CDS             8662..9444
                     /locus_tag="MIW95_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3153246.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            9821..10357
                     /locus_tag="MIW95_004009"
     CDS             9821..10357
                     /locus_tag="MIW95_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBY3153247.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            10389..10778
                     /locus_tag="MIW95_004010"
     CDS             10389..10778
                     /locus_tag="MIW95_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBY3153248.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            10815..11201
                     /locus_tag="MIW95_004011"
     CDS             10815..11201
                     /locus_tag="MIW95_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBY3153249.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
BASE COUNT         4055 a         1450 c         2160 g         3706 t
ORIGIN      
        1 atttggttcg atttttttgt ttaattggcc ataaaacaaa aagcctatag ttgaaaatgg
       61 aggatttttt atggattata aaaatatagc tcaagaaatt ctactaaatg taggaggaaa
      121 agagaatgta aatgaagtga ctcattgtat gacaagactt agatttaaag tcaaaagcgc
      181 ttctaaagtg aataaggata aattatctaa aactgaagga gtaataactg ttgttgagag
      241 tatgggtcaa atacaagttg taattggaaa taaagtaaaa acagtatatg atgaggttat
      301 aaaaattgta cctcaatcaa atgacgtagg taaaaagaat gaaagtcaag aaaagcaaag
      361 tataattaat tctatattat cagcagtagc tggtatattt acacctacaa tacctgctat
      421 agcaggggtt ggtatgatta aaggtatttt atcagttctt gctatgtatt atatgaataa
      481 aaatggagta gatataaaag aaacgcaaag ttatattatc ttaaatgcca tggccgattc
      541 tatattctat tttatgccta ttatactagg atatactgcg gctaaagtat ttaatgcaaa
      601 taaaataata tctatggtat taggagctac actttgttat ccaactttta ctgctttgat
      661 ggcaggtgaa gaatctgtta gatttttagg attggcagta acaaaagcta cgtatacatc
      721 atctgttatt ccaataatta ttgctatatg ggctctttca tacgttgaaa aagtattaga
      781 aaagtatata cctgaggtaa ttaaaataat aatggttcca actttatcat tggttgtaat
      841 gttacctgca acattatttt tatttggacc aatcggaatt tatataggaa atgtcataaa
      901 cttttcttat aagtatattt atgaattaag cccagcgttg tgtggagcat ttgtaggtgg
      961 gttatggtgt gtgttggtta tatttggtgc tcatagagca ttattaccaa taggtataag
     1021 tgatgttgct caaacaggaa gacagaattt attggcattt gcaggagctg caaacttttc
     1081 acaagcaggg gctgctctag gagtattctt taaaactaaa aatcaaggac taaaaacaat
     1141 ttctatgtct gcaactataa cagctttatt tggtataact gaacctgcaa tatacggtgc
     1201 caatcttaga ttaaaaaaac ctatggtatg tgccgtaata tgtggtgcat ttggaggtgc
     1261 gataatggga atgggtggag cttatggtaa tgcatttgca aaccaaggtg ttttaactat
     1321 acctgtatat gctgaagcag gtgcattagg tttcttaagt tatctaggtg gttgtgctat
     1381 agcatttttt ggttcagcta tttcaactta tctagttgga tttgaagatt tggaagagtc
     1441 ttctaataag gaaaattcat ttataaaagt agaaacgaag gatggtgtta ttgatataac
     1501 ttcacctgta gagggcgaat gtatagaatt gtcagaagta aaggatgatg tatttgcttc
     1561 aaaagctatg ggagaaggta tagctgtgtt acctacaaaa ggagtaataa cagcccctac
     1621 tgattgtgaa gtagcttctt tatttccaac attacatgca ataggattaa aattagataa
     1681 tggagcagaa atgttaatac atgttggaat caatacagta gagttaaatg gtaaatactt
     1741 tacaaaacat gttaatcaag gagatttagt gaaaaaaggt gataaactta tatcgtttga
     1801 tatagataaa ataaaaaagg ctggttatga tgtaactacg ccagttatag taaacaatac
     1861 atttgatttt gggcaagtag tttcttgtaa atctagttat gtttcaacca atgataatat
     1921 aatttcatta gtattagctg gtaattaagt taatttgact agagataagt aaattgattt
     1981 aaaaggaaat aagtaaatca acaattaaat acatctaaaa gtaagtttat tgcaatgaat
     2041 tgttaaagaa aataattaat atggagtaaa gctattgcaa tgaagtaaca aagaaaataa
     2101 ttaatataga gtaagtttat tgtaatgaat tgttaaagaa aataattaat atagggtaaa
     2161 tctatctcaa tgaaataaca aagaaaagag ttaatataga gtgaacttat tgcaatgaat
     2221 caacaaaaaa aataatcaat ataaaaagga gaaatattta tgaatacagg atttccaaaa
     2281 gactttttat ggggagcatc atcttctgca tttcaagtgg aaggagcttg ggataaagac
     2341 aataaaggaa aaaccgtagc cgactataat tcttttaaaa agtctcattt acaggcagat
     2401 actaaagttg catcagactt ttaccataat tatgaagaag atattgaatt aatgaaagaa
     2461 ttagggatga aaacatatcg tttttcaatt tcatgggcaa ggataatacc agatggtgaa
     2521 ggagagataa accagaaagg tttagacttt tataataaga taatagacaa actaatagag
     2581 tgtgatattg agccattcgt aactttatac cattttgacc ttccatttaa attagtagag
     2641 aaatataatg gttgggaaag tagagaaact gtctatgctt ttgaaagatt tgctaaaata
     2701 tgctttaagc attttggaga tagggtaaag tattggcaac ctcataatga acagaattta
     2761 attgttagag tggaggagag aattaatata tatgatgaaa cagattcatg gaagatagat
     2821 aaaatgcgtg ctcagatgga ttataatctt tgcttagcac atgcattagc agtcaatgca
     2881 tgtcatgaga tgataaaaga aagtaaaatt ggagcagcag tatcatcatc tgtaacatat
     2941 cctcttactt ctaagccaga agatgtatat gcagctagaa tgaatgataa ttttaaagtg
     3001 tactatatgc ttgatatgca tcattatgga gaatatccag ggtattatat gaagtatctt
     3061 gaaaaaagaa atacagttcc tcacatggaa gatggggata aagagatact taaaaaagct
     3121 aaaatggatt ttatagctgt aaattattat aggacaaatt gtgcagaagc tttacctgaa
     3181 gattcacaac atccatttgg attaagagaa ggaactgttg attttagtat gtatggacta
     3241 tttaaaatgt caatgaatcc taacttagag gcttctgagt atggagcagc aatagaccca
     3301 tcaggtctta gagttgcctt aaatgaatat tggcaaagat atcacttacc tgttataata
     3361 acagaaaatg gtctaggagc taaagatata ttagaagatg gaaaaattca tgatgactat
     3421 cgtatagact atttaagaag tcatataaat gcatgtaaac tagctattga agatggtgta
     3481 gagatgattg gatattgtcc atggtcattt acagaccttt taagttcttc tcaaggtttt
     3541 aataagcgtt atggacttgt ttatataaat agaactgacc atgaagtttt agatttaaaa
     3601 agaatcaaga aagatagttt ttactggtat aaagaagtta tagagaacaa tggaatagta
     3661 aaataaacat aaatatatat tgagggtttt agtatgtaca ttacaaaggt attaaataat
     3721 tcgttgctac ttgcaaagga tgataatgat gaagaaataa tattgatggg gaaaggcatt
     3781 ggccataatt ataaggccgg gtctgagtta aataaaaaag atatagaaaa gatttttgtt
     3841 cttcatgatg aaaatataaa aaagagtatt atacaattgg caacagaaat agatgcagaa
     3901 tattttggta tagctcaaat gataattgca tatggaatag aaaaatataa cttaaaactt
     3961 atgaaccata tatatttggc cttaactgac cacattgcct ttgcagtgag acgctttaaa
     4021 gaaggtataa tgattgaaaa tcactatttg ttcgaaatca aggaatttaa tccaaaagag
     4081 tatgatatag gaaaatatgc tattactgtt tttaaggagg ttcttggttt agacctacca
     4141 gaagaagaga ttggtaatat agcaggccat tttataaatg cacaacaaga taatccttat
     4201 agtgatagaa ataagagaag tgctaaaatt gtaaatgcta ttttgcaaat agttcattat
     4261 cacttttcta ttgtatataa taaagagtca ttttattata ggagatttgt aatgcatcta
     4321 aaggcctttt cacaaagatt tctggctaat gaaccatcaa gagaaaaaat agattttata
     4381 tatgagcaag ttcaaaaaaa ttgtaaggaa gagtatgaat gtgtgaaaaa ggtaggagct
     4441 tatatgtata aggaatactc cagagaatta ccaagacaag aggagttata tttgatgatt
     4501 catattcata agattttagg agaattatgc gaaaatagtt agaatatctt aatatgctag
     4561 taatattata aaaaattaaa aattaactac actttaaata tgaacaattg cattacttaa
     4621 gggggttgtc ttaaaattga tttttttaat taaatttgaa gctcaactcc ttttttaaac
     4681 ctttatatca ttacaacttt aatgtttata cgcctgtact ttagatgtat atctactttt
     4741 tacatttctc aacatgcaaa cctaccattt tttgcagtat gttaattgaa ataaaccatt
     4801 gagttaaccc taaatttatt ctggacttat aggaatagtt ccaaagatac tttaaaatat
     4861 tttctggaat aattgcttat ttcacatatg ttttattgtg atgttttgat ttaataaatt
     4921 cttcaactct gtatatagta aatgtattat catgtttgtt ttattcaagc acacttataa
     4981 tacgcttgat tttttcacaa tgatgaaata tactaatggg tgttataggt ataagaatcg
     5041 aattacactt gaagaagtaa gtgataaatg agatataaat cctcatatga taaactacta
     5101 tttcattgag gaacatattc taagtgatgt caagatacta caatatgact aaattcctag
     5161 tgataaaaaa gtctacgaat gagtgtagaa aggggaacta agatgaataa agttttaatt
     5221 atagatgatg ataaagatct ttgtattttg ttaaaaaaga gtatttcaat tgaacagatt
     5281 gaagcaaact attgccattc tgggagagat ggactggttg aactttcaaa acaagattat
     5341 cagttagtag ttctagacgt gatgatgcca ggtatggatg gttttgaaac tatggaacaa
     5401 atacgaaaat atagcagtat tcccatctta atgctgacat caaaaagcga taattacagc
     5461 aaagtgcatg gattgcgagc tggtgcagat gactatctaa ccaaaccatt tgaaattaca
     5521 gaatttactg ctcgtgtact gtctttaatc cgacgttaca cccgttttaa tccaaaaaat
     5581 gaggaaccac agcaactttc tttcaaaggt ttgttgattg accttgacca gcatacagtg
     5641 actaccaatg gcgaacctat gttgcttcca cccaaggaat ttggtgtgct tctttactgt
     5701 gcaaggcatc agggtaagat tttgacaaaa cagcagattt atgaagctgt gtggggtgag
     5761 ccatatgtat atgatgacag caatatcatg gctattatca gtcgcctaag aaaaaaaatt
     5821 gaactagacc catcatctcc catctatttg caaacaataa aaggaattgg gtatcaattc
     5881 aacacggagg tatgaaaatg gatgaacaaa cgataattct tttcttattc ttgctgtcga
     5941 ttggtctact ttttctttcc atatttgctt tttccaagct tgggcatatt cacaaacgcc
     6001 taaaagatat agaggagata ctggctgatg tacaaattgg aaacgaaaat cgcaaaatac
     6061 taattaagcc atgtgatgtt atggctccat tggtttatca gcttaatgag attgtctacg
     6121 attatgaaaa taagttactt tctctgaaaa agtcggataa agctagcaaa caacttatga
     6181 caagtctatc tcatgatgtt cgtacaccat taacaactct gattggttat ctggatgctg
     6241 tacacagtgg tatagttatc ggacaagaac gggaggaata ccttgaaatc gctaggcgta
     6301 gagcctacga cctcaaggat tacattgatg ttctttttga ctggtttcgg ttgaactctg
     6361 atgaatttac gctgtcaata gaatctgtgg aaattgcaga gttgtcacgc aatattttga
     6421 aagactggat tcctatattc catgaaaaga aactgaattt tgaaattgat attccagaaa
     6481 atcgactaat ggtaaatctc gacccagatg gatattcccg tgtggttaat aatctggttc
     6541 aaaatgttct tgctcacagt aaggctcggc aaatcaagat taccatgagt gaggattcca
     6601 gaatggtatt actccgtgtt gatgatgatg gtgttgggat tgccagagaa aatttaccac
     6661 atatttttga gcgactgtat aagtgtgata aaggacgttc agaaaaagga agtggtcttg
     6721 gtctatctat tgtcagtcaa atggtggagc gaatgggtgg tcagatttcc gtagaaagtg
     6781 agattggaaa gtataccgta tttacagtga gcttaccatt aatataaaat tgtttttccc
     6841 ccatgtgaca tgtatacaca gggggatttt ttgtaagtta taaagttgca aggttaatac
     6901 aaggttgttg caaggttaat gtaagattga ggtggtatga tagctttaat aaacaaagga
     6961 ggattttatt atttatgaat aatttaatta ttgaaacaaa aaatctcact aagcaatatg
     7021 gtgctcaaaa gagcgtttct aatttgaata ttcacgttca gaaaggtcgc atttacggat
     7081 tgctaggaag aaacggtgcg ggtaagacga caactatgaa aatgttattg ggtctaacaa
     7141 aaccaactac tggagaaatt aaaatatttg gtcaggacat tcacaataac gagaagaagc
     7201 ttcttcctcg aattggatgt ttgattgaat caccagggtt ttatcctaat ctgacaggaa
     7261 cagaaaatct caaaatcttt gcaaggctta gaggaatacc aaaaaaaaat gcagtagaat
     7321 cagcactgga tgtagtcggt ttgccaaaca atgacaaaaa gttattttct caatactctc
     7381 ttggtatgaa acagaggttg gcgattgctc ttgccattat gcatgaccca caactgttga
     7441 ttttggacga gcctatcaat ggacttgatc ctattggtat tgcagaagta agaaagttta
     7501 ttcgtgatct ctgcacagaa cgaggtaaaa ctattttgat ttccagtcac attctttctg
     7561 aaattgcctt gttggcagat gacattggta ttattgacca tggtattttg ttggaggaag
     7621 aaagtcttgc ccaattagaa gaaaaaaaca gtaaatacat acattttgct gtatctgatg
     7681 cagcacatgc agcaaggctt ctggaaaccc aacttaacct gaaaaatttc aaagtagcag
     7741 ataattacaa tattatgcta tatgatacag atgtttctgt tgcagaaata aatcgtgctt
     7801 tcattctaaa caacattgag gtatcagaat ctcatttatg cgaagatact cttgaagatt
     7861 acttcaagaa agtaacaggg ggtgaaggaa ttgcttgata taatttcatg tgaattttca
     7921 aaactcaagc ggttaaaatt tatactgatt tctattttag gtgcttgctt atttccaatt
     7981 cccgcaacaa ttttgattgc aaaggacaac ttgccctttg agcaattgtt caaattagtt
     8041 gtcaattttg gatatttttt gctattacca attgtattaa gtattgttgc tagccaacta
     8101 ttttttatag aaagagataa cgatgtatta aaaaatcttg caacagtgcc agtgccaaaa
     8161 ggaaaactgg ctctggcaaa attggctgtg ttgctgttta tatcattgtt ctactccgtt
     8221 gcagggttag gagctactat cattggaggt ttcattgttg gaatagtaga gggggttact
     8281 gtaaaattag gaatgagcat tgcttttgga attatgtttt ttgttactgt acttcctgtt
     8341 gtggtactca ttgtatattt caatcgcagc tatatttttt ctattatact ttcttttgta
     8401 tatgctattt tcaattttag tatatcctta aatattatca attttgaacc aaataaccca
     8461 ctattaagcg tattacctgc tcctgttata atgcgttggt ggatggcatt ttggggagat
     8521 cccactggcg aatacacggc acttagacag ccatatttgc tctcaactcc tgcttgtgta
     8581 ggtattttat gtttgataac cattatagca gtcctgctga tttgtatgat atacaaaaag
     8641 caggagaatt aggaggtatc tatgcttgat ataattacta cagaatttca aaaaataaaa
     8701 agatataata ttctatggat tggtgttgtt gctgttctgt tttcagcact attggcagtt
     8761 tttcaggttg taagttcaca cggcagtgac cctctaacgt atgagagctt tgcaaacgga
     8821 gttatctgga ataatttcag tcttagtttt ccatttggga ttacacttat tggaggatat
     8881 cttattaacc gtgaatatac agaccaaacc ttaaaaaaca tcctaactgt tcctatttca
     8941 ttacgcaaat tgctagtggg aaaattaatt gcggttggag gaattgctgt attgttaagt
     9001 cttttcagct tcttatgcac atttattttg gggttgttat tttgcaatat caatatatct
     9061 gttacactca ttgtaaaatc cttagttcag attttgtctg ttaatctgta ttgttatgtt
     9121 gctgttttgc cgattattgc ttattttggt cgtaaacaaa atgcatttct gacgggtgta
     9181 gggattgcat ttgtatacgg gttttgtggt gtgtttgttg ctggaagaaa tctgacagac
     9241 ttttatccaa tcacagcagg tcttggtcta gttggataca acaatggtgc tggtactgtt
     9301 tatcagccac ttatcggtgc tatgactttg attgtaattc taatcttgac aactattcta
     9361 cttgtattta cacctaatta tgataaagtt atgaccatat ctaagaaaaa agggataaag
     9421 cataaaaagt attctcataa ataacataaa tattgtttct attatttaag ttgctagaca
     9481 gtgaaaagaa aaaatctgtc gtacagtggc tatatgctca tgtattcaag aagctacagt
     9541 tttgattttt cataactgta gcttcttttt gtatttatta aatcacggct atcataatac
     9601 ttgcaatagg tatagcgaca agagagagta ctgtcatgtg tgttttatat attacattaa
     9661 gtatattcta aaatatatgt tttaaataat atatttgaaa ataggaatat ttaaataaaa
     9721 aaaataatat tatatatagt tgacaaaaat tggcaaatga tataatatat aaatatagaa
     9781 atagtaatca ttactattat taaaaaggga ggaattaatt atgaacttaa aaggaactaa
     9841 aacagaaaag aatttaatgg caggatttgc aggagagtca gaagcaagaa ataaatatac
     9901 atactacgct tcaaaagcaa gaaaagaagg ttataatcaa atagctgcta tatttgaaga
     9961 aactgctaac aatgaaaagg aacatgctaa attatggttt aaacttttac atgatggtat
    10021 gccttcaaca gaggaaaatt taaaggatgc agcagcaggt gaaaactatg aatggactga
    10081 tatgtatgca aaatttgcta aagaagctag agaagaagga tttgacaaga tagcttattt
    10141 atttgaagct gttggaaaaa tagagaaaga gcatgaagaa agatacttaa agcttttaga
    10201 aaacttaaat gaaggaaaaa tatttaaaag agatgaagaa gttgtttggc aatgtcaaaa
    10261 ctgtggtcat gtgtatgtag gtactgaagc accagaaaaa tgcccagtat gtgaccatcc
    10321 aaaagcgtat ttcaatataa aagctgaaaa ttattaaaaa ttatcattat taaaaaggaa
    10381 tgttaactat gaaattttct aaacaacgag aactgatttt aaatgaaata ttaaataatc
    10441 cggttcatcc tactgcggat tacctatatg aaaacttaaa aaaagataat ccaaatttaa
    10501 gcttaggaac tgtttataga aatttagctc agctaacgga acatggcttt ataagaaagg
    10561 ttagtattcc agggtatcca gatagatttg atggtcgaat agataatcac tatcatatca
    10621 tatgtgaagt atgtggagag gtatacgatt tagaatccga agttcttaat aatttacaag
    10681 aattaatatc tgaggagaca gacataaaaa taacatctta taacataagt tttaaaggga
    10741 tttgtaataa ttgtaaaagg tgtagccaag taggttagtt ttcaaattct aaaaataatt
    10801 aatgaggtga gtttatgtgt agtgaacaaa aattttttat atgtgagact tgtggaaacc
    10861 ttgtaggaat gatacaaagt ggtggtgtac ctattttctg ttgtggaaaa cctatgaagg
    10921 aacttgttcc taatacaaca gatgcagcag ttgaaaaaca tgtacctgta attgaagtgg
    10981 atggaaataa tgttactgtt aaagtaagta gcacaactca tccaatgact aaagaacatc
    11041 atatagcatg ggtgtatctt atgactgaac aaggtagtca acgcaaatgt ctagcagtag
    11101 atggagaacc tgtagttaaa tttgctttga atgatgatga taaagtgatt tctgcctatg
    11161 cttactgtaa tttacatgga ttatggaagg ctgaactgta atatttaaat aaattgagtc
    11221 aaagaggagg ctataaatgt attttcatac aaatagcctc ctataaattt atataaattc
    11281 aagttttaat tttgtttaat gtcaatataa tataaggaga aaataatatg tcaatttata
    11341 aatgtagtgt ttgtggatat atttatgatg a
//
LOCUS       DAGQWR010000354         8302 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.guided.166, whole genome shotgun sequence.
ACCESSION   DAGQWR010000354 DAGQWR010000000
VERSION     DAGQWR010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8302
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.guided.166"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..1599
                     /locus_tag="MIW95_004012"
     CDS             <1..1599
                     /locus_tag="MIW95_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBY3153250.1"
                     /translation="VLFGTPTILGDALKPIWDILISLNPVLHGGKVASVFGSYGWSGE
                     GIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSYTGKFLDKLNSTFGSKKKTKKF
                     KCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVTFRKDTDEYFVIVGNGAAGFYA
                     ADAIRKRNKTCKITMISNEDELTYYRPALSDGINEELGSDFYMEDKDWYDKNNIVVIL
                     GTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIPPIKGHDLENVFTLRNIKDLYS
                     VKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVVEAMDSILSKQLDKEGSKILEQ
                     CVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDCDMVVFSIGVRANTQMVQDTSV
                     KIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWPSSVEMGKIAGANASGDNLTFE
                     SEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRIYKKLFMKDGSLVGAILINDLS
                     CTVKLIRLISEKGDFEDIMKADIL"
     gene            2033..2734
                     /gene="vanR"
                     /locus_tag="MIW95_004013"
     CDS             2033..2734
                     /gene="vanR"
                     /locus_tag="MIW95_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBY3153251.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            2784..3866
                     /locus_tag="MIW95_004014"
     CDS             2784..3866
                     /locus_tag="MIW95_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY3153252.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            4127..5227
                     /gene="vanG"
                     /locus_tag="MIW95_004015"
     CDS             4127..5227
                     /gene="vanG"
                     /locus_tag="MIW95_004015"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBY3153253.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            5224..6030
                     /locus_tag="MIW95_004016"
     CDS             5224..6030
                     /locus_tag="MIW95_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBY3153254.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            6048..8186
                     /gene="vanT"
                     /locus_tag="MIW95_004017"
     CDS             6048..8186
                     /gene="vanT"
                     /locus_tag="MIW95_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBY3153255.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT         3070 a          894 c         1509 g         2829 t
ORIGIN      
        1 gtattatttg gaacaccaac aatattagga gatgcgttaa agcctatatg ggatatatta
       61 atatcgttaa acccagttct tcatggtgga aaagttgctt cagtatttgg ttcatatgga
      121 tggagtggag aaggtataga aaatgctatg gagagaataa gtcagcttag aatgacagca
      181 gtaaaacctt ttgctgttaa ctttaaacca tcaaatgaag agattgataa attacattct
      241 tatacaggta aattcctaga taagttaaat tctacttttg gaagtaaaaa gaaaactaag
      301 aaatttaaat gtgtaatttg taatgaagtt tttgaaggtg atagtgctcc tagtgtatgc
      361 cctgtatgtg gagctaagga agaccaattt atagaagttg aagaagatga agttactttt
      421 agaaaagata ctgatgaata tttcgtaata gtaggaaatg gagcagctgg tttttatgca
      481 gcagatgcta taagaaaaag aaataaaact tgtaagataa ctatgatttc taatgaagat
      541 gagttgactt actatagacc tgcactttct gatggtataa atgaagaact tggttcagat
      601 ttctacatgg aagataaaga ttggtatgat aaaaataata tagtagtaat attaggaacg
      661 aatgtagata aattagatga agtgaataaa actattatag tgaatgatgg agctattaaa
      721 tttgataagc ttgtaatagc tacaggaagt agaaacttta taccaccgat taagggccat
      781 gatttagaaa atgtgtttac tcttagaaat ataaaagatt tatatagtgt aaaagaagca
      841 ctagaaaaat ctaagaaagt tgtagtgatt ggtggcggtt tattgggact tgaagctgca
      901 tgggaattta gactaaaagg attagaagta gtagtagttg aagccatgga tagcatacta
      961 tcaaaacaac ttgataaaga gggtagtaaa atattagaac aatgtgtaag agatacagga
     1021 atagatgtaa gattgggtgt agcagttgat ggtattgaag gtgatggaaa agctcaaaaa
     1081 gttgtattta aagatggaga tagtgttgac tgtgatatgg tagtattttc aattggtgta
     1141 agagcaaaca ctcaaatggt tcaggataca tctgtaaaaa ttgatagagg tattgttgta
     1201 gataagacat tacagacaaa tgttaaagat atatatgctt gtggagatgt tgcacaagtg
     1261 ggaaatatta gtttagcaat atggccatct tcagtagaaa tgggaaaaat agcaggagca
     1321 aatgcaagtg gagataattt gacatttgaa agtgaagtat atccagtttc attagatgca
     1381 atgaatgtaa aagtatttag tattggtaat atacaaaatt ttgataaaga aatttcatca
     1441 aaagatgaag gtcaaagaat atataaaaaa ttatttatga aagatggttc attagttgga
     1501 gctatactta taaatgattt aagctgtact gttaaattga taagattaat atctgaaaaa
     1561 ggtgattttg aggatattat gaaggctgat atactataat ttaaatacat taaaataaaa
     1621 aggagttgtc tcaaaattga tttttttcta atcaaatttt aaattcaact ccttttttga
     1681 atttttatat aaaataacat ggaaatttta gtaatgtctg tggttacata cataggttta
     1741 attgtttttt atatggattg tctctggtat aataaattga attgtgtttg actgatagat
     1801 atttttattg aaagttaata cctgttatgc gataataagg acgtgttagg ggagttttta
     1861 taataacaat taatctcagt ttttgtgttc caatagtagc atcaacacat tctgatgtag
     1921 tagttctttt cttataaaaa ttagttacaa atatgcttgt aattatttga tatgtaaatt
     1981 taccaatatt ataaatgatg acctcgataa taaggagagt gaaaaataat ctatgaacga
     2041 aaatattttg attgtggatg atgaaaaaga aatagcagat ttaattgaag tatatttaaa
     2101 aaatgaaggt tttacagtat acaagtttta tacaggtaag gaagctctag aatgtattga
     2161 atctaaagat ttggatatgg ctattttaga tattatgctt cctgatattg atggatttca
     2221 tatttgccag aaaattcgag aaaaatacta ttatcctatc attatgttaa cagctaaaat
     2281 tgaagatata gacaagataa tgggtttaac cataggtgct gatgattata taacaaagcc
     2341 atttaaccct ttggaagttg ttgcaagagt aaagacacaa ttaagaagat atatgcgata
     2401 taataattct tatgaacaac agtcgataat tgttaatgag tatgatatta aaggattgat
     2461 tatcaataaa gaaacacata aatgtagttt atttgggaaa gaagtagcat taacaccgat
     2521 tgaattttct attctttggt atttatgtga acatcaagga aaagtggttc cttctgaaga
     2581 attgtttgaa gctgtatggg gagaaaaata tcttgataat aataacacag ttatggctca
     2641 tatagggcga ttacgtgaaa aaattcatga accttctaaa aacccgaaat ttataaaaac
     2701 tgtatgggga gtaggttata caattgaaaa gtaaaaatat taaagtcaat cctgacttta
     2761 ctcaagtgag aaataaaatt tttatgagaa cagctttaat ggcatttata gccattattt
     2821 tcatatggat tttatattct tttatattgt atggtaagtt ttctatctgg atagtatcat
     2881 tctttgaaaa tgcttttcgt atggaatata aagctgcatt gaatttatat cagcagattt
     2941 tccgtaatag catggagctc ttcatgatac tagctatggt gattgtgttt tttataatat
     3001 tccgtgttta tttaaattgg tttacaaaat attttagtga aataaatcaa ggtattgact
     3061 ctcttatcaa agaagatgtt ggagaagtgg ctttatctcc agagttattg gcaatagaaa
     3121 agaaaataaa ctcaattaaa catatacttg aacaacgtaa atttgagaca caaatggcag
     3181 aacaacgtaa gaatgaactt attgtttatc ttgcacatga tttaaaaaca ccacttacat
     3241 cagttatagg gtatctgaca ctacttcgag atgaaagtca gatttctgaa gaactgcgaa
     3301 aaaaatattt atctatttcg ttggataagg cagagcgtct tgaagattta attaatgaat
     3361 tctttgaaat tacaaggttt aatttatcaa atatatcact tgaatacagt acagtaagct
     3421 taactcgtat gcttgaacaa ctaacatatg agtttaaacc tactttgatg agtaagaatt
     3481 taaaatatac actgaatgtt acaccaaata tgatgataag gtgtgatgtt aataaaatgc
     3541 aacgtgtatt tgataactta ttacgaaatg ctgtttatta tagttttgaa gatactacta
     3601 ttgaaataac agctgtacag gaggatgatt atataaagct taaatttata aatcatagca
     3661 atacgattcc agaggaaaaa cttgagcgta tttttgaaca attttacagg ttggatacat
     3721 ctcgtaattc aaacagtggt agagctgggt tgggacttgc tatagctaaa gaaatagtgg
     3781 agcttcataa cgggacaata acagctcata gcgagaacaa tataattacc tttaaggtta
     3841 ttattccgat tgtaggaaat ttgtaagaat ttaagtataa aaaaatagga aattctttag
     3901 gataaataaa taaagagcac tcttgttatt gatataatta ctgtatcaaa acaagagtgc
     3961 ttttattttg atacagtaat tatatcaata gaaaaatgtg aaaatatatt atgtggaaca
     4021 gatgcaattg taagtgaagg ttgattggat ttgaaataga aaaagtattt taacatgttt
     4081 ctgtagattg tataaaaata aataaatcaa caaaggagaa tcaaagatga ataataaaaa
     4141 aattgcagtt atatttggtg gaaactcaac agaatatgat gtatcattac aatcagcttt
     4201 tgcagtactt gagaatttga atacggaaaa atatgatatt ataccaattg gaattacaag
     4261 ggagggtaat tggtatcatt atattggaga ttataataat atccagaata acacttggtt
     4321 tgaaagggat actgatataa ttccagttat tgtttcgcag aaccgtgtca aaaatggtat
     4381 tattgaattt ttaattcctg atgctgtttc tcagaatcat attccaaata gtgctattga
     4441 atcatgtgaa aatgatatta aatacactaa acttgatttt gtatttcctg tattgcatgg
     4501 aaaaaatgga gaagatggaa cagtgcaggg gttatttgaa ttggcaggta tacaggttat
     4561 tggatgtggt actctttctt cagcattatg tatggacaaa gacaaatcac ataaacttgt
     4621 tcattcagaa ggtattaaag taccaaaatc aatattacta aaatactcag ataaatttaa
     4681 tgtggctgaa ctgtcaaaag acttaactta tccattattt ataaaacctg tgcgtgcagg
     4741 ttcatcattt ggtattagta aaatttataa aaaagaagag ttacaagtag cagtagactt
     4801 ggcttttgaa catgataatg aagtaataat tgaggaaaat attgatggtt ttgaggtagg
     4861 gtgtgcagtt ttaggaaacg atgacttact tgttggaaga gtagatgaaa tagaattgtc
     4921 aaaaggattt tttgattata cagaaaaata tacattgaaa agttcaaaaa ttcatatgcc
     4981 agctagaatt gatttggatt gtgagaaaag aatacaagag acagcaaaaa ttatttatag
     5041 aactcttgga tgttctggat ttgctagagt agatatgttt ttaactccat ctggtgaaat
     5101 tatttttaat gaagtcaata caattcctgg ctttacttct catagtcgtt atccgaatat
     5161 gatgaaaggt attggactgt cttttgaaga aatattggat aagataattg aggtgtacat
     5221 aaaatgatga ataatatttt attagataaa aataaagaag ataaaatgac aaatactatc
     5281 ttattgacta aagataaagt ttatgatggg aatctaattc tagtaaatgc ttttttacca
     5341 gttaaaacat ctgaagatat agatttaatt cctgtagata caaggtttcc aagtattctt
     5401 atgaaacgtg aagctacaaa tattttacaa aatattttaa aaagtatatg tggaataaat
     5461 gaaatagtac ctgtaagtgg atatcgtact gcagaggaac aacaagatat atattcaagc
     5521 tctctgcgtg acaatggaaa agactttact aaaaagtttg tagcacttcc aaatcacagt
     5581 gaacatcaga cagggcttgc tattgattta gggttgaaga aagaggttat agattttatc
     5641 tgccctgaat ttccttataa tggtatttgt aatgagttcc gtaaggcagc actacattat
     5701 ggttttatag agcgttatca gtgtaaaaaa gaagaaataa caggaatatc tcaagaacca
     5761 tggcattttc gttatgtcgg atatccacat tctgagatta tcaataaaat gaatctcgcc
     5821 cttgaagaat atatagaata tattaagaga ttttcgtata caaattgttt aaaaatagaa
     5881 gataaagaaa aaattataga aatcttttat gtgcctattc atactaaaga agaagtgaga
     5941 atctctatac ctgaacaatg tgtatatcaa atatcgggta acaataagga tggttttatt
     6001 gtgacatttt ggagaaatag aaaggactaa gaaatggagg aacaagaatg attaggaata
     6061 aatcttatgc agggcttgat tattttagga ttatagctgc attacttata attgcaattc
     6121 atacatctcc attattatca tatagtaaaa tggctgactt tgtgttaact agtataattg
     6181 ctcgtattgc tgtgccattc ttttttatgc tatcaggctt ttttctaatt tcaacatata
     6241 attataattg taataaactg aaatcttttc taaaaaatgt attatttatt tacgggatag
     6301 ctattgtaat ttatattccc ttaaatattt acactggata tttttccatg aataatttag
     6361 tgcccaatat tattaaggac attgtatttg atgggacttt atatcattta tggtatctac
     6421 ctgcatcaat tattggtgca agtattgcat actttcttgt aaaaaaacta ggtttaaagt
     6481 actcattagt aatcactatt gttatttatg taattggtat gtttggtgat agttattatg
     6541 gttttttaga aaaaatacca ctattaaaga atttatatgg gtatattttt gaagtatcag
     6601 attatacacg aaatggtata ttttttgcac ctgtattttt catacttggt ggaataatat
     6661 ctaataaatc taattatatg tcaactaaaa atagcttgat tggatttctt atatcattct
     6721 tacttatgtt atgtgaggga atgtttttac ataaactagg ggtacagcgt catgatagca
     6781 tgtatattat gttattgcca tgtatgtatt tcttatttac agccttaaca tttttaaaag
     6841 gttctcgagc taggttattg cgaacatcat cattaattat ttatattata catcctatga
     6901 tgattgttgt tattcgtatg ttttctaaaa tattcggaat acaaactgtc ttggttgaca
     6961 atagccttat tcattatata gtggtaagta tagcatcttt tatagcttct ataattatta
     7021 tattgattct taaacctcta aaaaaagact tgaaaggaga acataaggta aataccaata
     7081 gagcttggat tgaaattgat ttaaataatt tggaacataa tgtaaagatg atacaaaaag
     7141 ctatgccaga agattgtgaa ttgatggcag ttgtaaaagc taatgcctat ggtcatagtg
     7201 cttttgaagt agcgacttgt gcaaaccaaa taggtgtaaa agcatttgca gtagcaacaa
     7261 tagatgaggg gattgattta cgtcgatatg gaatactagg agaaatattg attcttggat
     7321 atactaaccc aataagggca aaggaacttc ataaatacaa tcttacacag agtgttattg
     7381 accttaatca tgctatctct ttaaacaaac agaaatacaa cataaaatca cacattaaaa
     7441 ttgatacagg tatgcacaga ctgggttttg atgttaatga tgtgaggtca attttaaagg
     7501 catttaaatt aaaatactta gatatatgtg gcatatttac acacttgtgt gtttcagata
     7561 gtctgaaaga tgaagatgta gactttacaa ataaacaaat tgaatgtttt tataagttaa
     7621 ttgattttct gttaaaaaaa ggtataaaaa ttcctaaagt acatattcaa agtagttatg
     7681 gtttttttaa ttatcccaat cttaagtgta attatgtaag aattggaatt gcattatacg
     7741 ggatattaag ttctccaaat gatacgacta aattgcaact tgatttacga cctgttttat
     7801 ccttaaaatc acaagtaatt cttattagaa aaatacaaaa agatgatagt tttggatatg
     7861 gaagagtctt tattgcaagc agagatagcg taattgctat attatctatt ggctatgccg
     7921 atggtatacc tagaaattta tcctgtggaa aaagttacgt aataataaat ggttgtagag
     7981 cagcaatagt gggtcgaatt tgcatggacc aacttgctgt tgatattaca gatataccta
     8041 atgtagaagt tggtaatacg gcaataataa taggaagaga taatttatct gaattatctg
     8101 cttctgaagt tgcaaataac tcatgtagta tatcgaatga attattgagc agagtaggaa
     8161 gaaggttaaa tgttataaag aagtaactca attaaatagt aatatttgtt gtactgtgta
     8221 atttatttta ctctgactag acatttttag taatattatg tctaatatat attttttata
     8281 acttacatga caatttaagt ta
//
LOCUS       DAGQWR010000355         7862 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.guided.173, whole genome shotgun sequence.
ACCESSION   DAGQWR010000355 DAGQWR010000000
VERSION     DAGQWR010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.guided.173"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            82..798
                     /locus_tag="MIW95_004018"
     CDS             82..798
                     /locus_tag="MIW95_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153256.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSESVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            826..1764
                     /gene="blaCDD"
                     /locus_tag="MIW95_004019"
     CDS             826..1764
                     /gene="blaCDD"
                     /locus_tag="MIW95_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBY3153257.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            2108..2791
                     /locus_tag="MIW95_004020"
     CDS             2108..2791
                     /locus_tag="MIW95_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3153258.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2788..5448
                     /locus_tag="MIW95_004021"
     CDS             2788..5448
                     /locus_tag="MIW95_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3153259.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5629..6810)
                     /locus_tag="MIW95_004022"
     CDS             complement(5629..6810)
                     /locus_tag="MIW95_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY3153260.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6810..7472)
                     /locus_tag="MIW95_004023"
     CDS             complement(6810..7472)
                     /locus_tag="MIW95_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY3153261.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVLLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(7617..>7862)
                     /locus_tag="MIW95_004024"
     CDS             complement(7617..>7862)
                     /locus_tag="MIW95_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY3153262.1"
                     /translation="FFSFNKFMFDEIQGMFEIGKNDRSIRHDIEIPTLMYSSIFTLTG
                     FFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
BASE COUNT         2939 a          963 c         1255 g         2705 t
ORIGIN      
        1 ctattgtaat atttaaatat tacaatagta agttttaaat agagaataaa taattacctc
       61 acaaactgaa aggaggacat aatgatacta atagttatat tgagtatatt catcatagcc
      121 tcaatcaaca taattatatt aggtaaagaa aatataagag atatagttat ccctttaact
      181 ctatttattg taagtgattt tttatttttg tgtggaaata taatatgtag gggaggagta
      241 atgctactat ttataggttt atttgcaata ccattgatgc taattgcatt tatatggatt
      301 tgtgtacata tgattaagct ttactataaa aaagatttaa ataaaagaaa aatgattgga
      361 tgtatattta gtatctttat aagtataggt actatattta ttcctagtcc aagtgaaagt
      421 tttagattta aactttatga aaaagattat agtgtagtag ctaaagctat tttgaaagtt
      481 tatgatgaaa aaaagttaag ctctgaatct gtcttttata ttcaatctga taaaaatgaa
      541 ttggagaaga tattttctaa agaagtaata aaaaaaatga aaaaactaaa cagaaattta
      601 gatgtagtta aaatttctgg gaatagaaat ttagtctatt ttttctttgg agctgaatta
      661 caatctattg atggtattgt gatttttaaa gatttaaaac atgttaaacc aaagttagat
      721 tttataaata gaagtattca agctcctaga ttggagtata ttacaaataa tgtatattat
      781 tttaaaggtg aactataaac ttatgtagag gagatggtat aaagtatgaa aagaaaaaat
      841 aattttattt ggatagttat tcttctagtt gttgtagcta tgggagtata ttatgtagga
      901 agaaagcata acaacacaaa acaaaaacat gataagaatc atataaactc tgaattaaat
      961 aaatccaaag ttgaaaacaa tgataaaaac aaaaaagaaa aaattaatat ggttgactat
     1021 agtgactgtt ttgaaggtat tagtggagga gctatttttt ataatactaa aaataaagaa
     1081 tataatatat ataacaaaga attaattgaa acaagaaggt caccatgttc tacttttaaa
     1141 atagtttcaa ctttaattgg tttagaaaaa ggggtaataa actcaaaaga atcagttatg
     1201 ggatatgatg gtacagagta tccaaataaa aattggaata aaaatcttag tttagaagag
     1261 gcatttaagg agtcttgtgt ttggtactac aaaaaattga tagataaagt tgatgctaaa
     1321 tctgtccaaa acattttaga tgatttaaag tatgggaatt gcgatattag cgaatgggaa
     1381 ggagatttaa aaaatggaaa gggacattta aatggatttt ggttagaatc ttcattacaa
     1441 atatctccaa aagaacaagt gcaaacaatg gcaaaaatat ttgagggaga tacaaacttt
     1501 aaaaaagagc atattaatat tttaagagat attatgaaaa tagatgtaaa tgataaaaac
     1561 ataaatgtat atggaaaaac tggaacagga tttgatgaaa aaaataaacg tgtggatgca
     1621 tggtttgttg gtatgctaga gcgtgaaggt gacacctatt attttgctat taaatcagat
     1681 gattccaata aagaaattac agggccaaag gtaaaggaaa ttgcaattaa tataattaag
     1741 aaatattata gtgtaaggga ataaacttaa ttttatttgt aaatattatt tacctatagt
     1801 ctgtaatatt tattaaataa ttttactaaa ttgagtctat atagagcaaa actcctttag
     1861 tacataaaat acttgctatt gattattaaa ttgaatacct atagatttaa agttaatacc
     1921 ttttaaagac tatgtaatat attaaaagta taaataaaat atattgcata gtttttgttt
     1981 tatattataa ttttaaataa tgtgcaaaaa ctaaacaata tttttaatag taatttatat
     2041 ttgtattcat gtcactataa tgtcatttta ggttgctaaa atttaaccat aaaggagatg
     2101 attgtatatg gaaatattaa aatgtgaaaa tctcacaaaa atatatggtt caaatcaaac
     2161 cagagtgaca gcccttaata atgtaaattt atcagttcaa aaaggtgatt ttgtatccat
     2221 tgtaggagca tctggttctg gcaaatctac attgttacat ctgcttgggg gagtagatag
     2281 acctacatct ggaaagattt atgttgaaga tacagaaata tcaagtttaa aggaagaagc
     2341 tcttgcagtt tttagacgta gaaaagtagg tcttatttat caattttata atcttatacc
     2401 tacccttgat gttagaaaaa atatattatt acccatgtta cttgataaaa gaaaggtgga
     2461 tgaggataga ttctctgaga ttgtatctat attgggatta tctgatagat taaaccatct
     2521 tccaagtcaa ctttcaggtg gacaacaaca aagggtatct atagctagaa gtttaatcta
     2581 tagaccagct attttattag cagatgagcc aactggtaat cttgatagaa aaaattcaga
     2641 agaaattgta gacttgttaa acttatcaaa taaacgattt aatcagacaa tacttcttat
     2701 tactcatgat gagaaaatag cactagaggc aaatcgtatt gtaactatgg aagatggagt
     2761 gattgtctct gaaaaggtgg tgaagaaatg aatattcttc aagaatacac cttagacttt
     2821 gtacgacgca ataaacgtag tagtatagct ataatgttag ctatattact tactacaacc
     2881 atgatgtctt ctttgtgtgg actgttgtat accatgtgga cagattttat aagactttca
     2941 attgagaaag aaggtaactg gcatggtgaa ttgtttgact atacttatgg aagtgactta
     3001 ccattaattg aaaatttttc ttcagttgat gatgtaatgg taaaaggaca atggtatgta
     3061 ggcaaaatta atgatgagaa aagagattat ataatttata gaaatgctac atcagagtat
     3121 tggaattcta tgccagaaaa ggatactata atagagggtc gtcgaccaaa taatgctaat
     3181 gaaattgcat tatcaaaaca atattttgag aataatccaa gtgtaaaaat tggggataaa
     3241 attactcttc caataggaga tagaatccta aatggaaagc ctcttgaacc catagctcaa
     3301 aaatcgaaag gggaaagttt taaaaaaatt tcagagacta cattgacaat agtaggaaag
     3361 gtagatgttt ccacttcatc agtaatacct gcatatacag ctcttggata tcttgataga
     3421 acaagtataa aacctaatga tagtataact gtgtacttgc gatttaaaaa tataagagac
     3481 acatacaagg aacttccaaa acttgctaaa tcattggggt ggaagactaa tgaatatgga
     3541 aaatacaatc ttaaatataa tagtaattat cttttaaaaa tgttagtctt ttccccagaa
     3601 cagaaagcat ctatgagcag tatatcaaaa ttttcaactc ctattatgta tcttacaata
     3661 gcaattttta cagtagctgt gtttgtaatg gtgatttaca acgcgttttc actatctgca
     3721 aatgctagac ttactcaatt agggatatta tctagtgttg gtgcatcacc aaaacagatt
     3781 aaaatgtctg tagtgtttga aggttttttg cttacaatta taccattgcc aataggttta
     3841 tttttaggat ggttattgtg taataggctt atagtttata ttaattcagt taattatcat
     3901 actgatgttc cagaagtggt atttacttat ggtattcctg cttttctgcc agcagtttta
     3961 cttacaatag taacagtctg gatttctgca cttataccag cacgaaaagt atcaaagata
     4021 tctcctatag aagcaatacg acaaggagat agtgttaaaa tcaaaaagtc gcataaatat
     4081 tcattaggaa agatatttgg aatagaagga gaacttgcat caaacgcact acgtgctcgt
     4141 aaaaaatctt atcgtactgc aacaatatct ttaacattat cttttttatt acttacatgt
     4201 tttttgcata taattgcaaa tcaagaagga gcaaaagctg tttatggaag tgataattat
     4261 aaggaccaaa gagatatagc agtttatttg gaaaatagtg aacctgtaga gtctgctttc
     4321 attgaaaaat tagataatac agaaggtgta aaaagttcac tatattatca aaaaatgtct
     4381 gcttccactt ggctgactga aaaagatgca tcaaaagaac ttgcgaaaaa tggaggattt
     4441 aaaagtatag tttcatctaa aaaatactct cctatagaac gtgatggcaa gtttcgtata
     4501 atttcaaata taattgggct tgatgataag agttttacag aatattgtag gcaaatttct
     4561 gtagacccaa agttatttta tgatacaaaa aatccaacct caattgtata taataaggag
     4621 gaagatatta atcgtagtac acgtagaaat aaagtatata tagattattt aaatctttca
     4681 ataggagata acattaaatt aaatgaaaaa gtatatgacg aagataaagg ggattatact
     4741 tacaacatga aagtgggagc tattacaagc acacttccta aaacagtaga tactagttca
     4801 aaatatacat taatgcaaat agtgccaatg tctatggtgc aggaaatatc taaaaattat
     4861 gatgaagtaa gtaggttacg ttcaaatagg ctgataggag tatttaaagt aaataatcgt
     4921 gaggacattc ctagagtaag agaaaaatta gagtccattt ataataaaga ctatggttca
     4981 ggagattata gtattgagga tgttttagaa agtgaaaaac aaaaggctag tggaataaag
     5041 accatgaatt taatagtagg ctttattaca ggtttattgg ctatgattgg attatcaaat
     5101 gtacttgcta cagtgtcagg aaatactaga agtagacgac aagagtttgc tatgttgcgt
     5161 tctgttggat tatctccaga aggcataaaa aagatgcttg ttttggaagg tttgttctta
     5221 ggaattacac cattactact tagtattcct gtgcaaatag gaatagtgta tgcttttcta
     5281 cgtataaatg aaatatattt tatagagtat ttaccatttg cacctatatc tacaataata
     5341 ggttttacta tattgatact ttttattgta atagcatcat atatgacagg atacaagcag
     5401 ttgaagaatg aaaatatagt agaatctata aaaaatgaaa caatataaac aaaagctatt
     5461 tgaacttatt taaaggtttc gatttaagac tacttacaag tatagggata gttaatttgt
     5521 ttgtttaata aaaccttaaa tgtaaataga agtatattac taaaacaata gataacatag
     5581 aatatagata tagctggatt ataggtattt tacctataat ccagcatttt atgtgtaaaa
     5641 tttaattatg aatcttgcac caccattatg gcaattttct gcatgtagag taccattttg
     5701 acgttctata atagactttg atagagcaag ccctatacca atactgtcct tactagcatt
     5761 tttaccacga taaaatctat taaaaatata tggaagttct tcctgagcaa accctttacc
     5821 attatcttct atgataatct caacatatag aggattttga ctataataag caactatata
     5881 tccaccatct tctatatgct cactacaatt tttaagaata tttaaaagag cttctaaagt
     5941 ccaattaata tcgatttcaa attctaccga aggagaagcc tcgatagtaa atgtctgatg
     6001 tttttctctt aaagaacctt ctataggctc aattgcataa gtaagaagag aatgtacatc
     6061 tataagtttg ctttcaaata ttaaagtact tgcatccaat tttgataatg taagtaaaga
     6121 tgaaactagt ctctcaagtc ttgagatttg atttgtaagt ctgttaatat aatctatttc
     6181 ttcatctgga caattttctg aaagtaattg tgccataagt gacatagaag taattggagt
     6241 cttaagttga tgagcaatat ctgcaagatt atctgaaaga gtttgtcttt cttttaaagc
     6301 aaattctttt gaacaacgaa gttccccaac tgttttatat atctcatctt caaggtgaga
     6361 aaattcatct tcacatcgta aaagtacaga ctcttttcct aaattcactt cttctaagta
     6421 ttgagtaaga ttgtttattc tactcagttt ttgcttattt gttctatgtt tgattatata
     6481 tacaactgta gccattgttg aaattatacc aaacgatatc actagaaaat aaaaaaagtt
     6541 tttactccaa aatgtacttt tagaatatcc atgttttgat aaaatttcat ttcctgtttt
     6601 aaaatcctta tcactagaat ttttcagtga ttttagtata ctttctgtag acttaggatt
     6661 gtactcatat gatgcaccaa gagccttaga cagtacattg tattcattaa aagaagttat
     6721 agttgttaga attacactta ataaaaaagt tataattaag cagaatatta taaaaacaac
     6781 tgtactgttg atatttcttg gagttttcac tagctgtcct ccattctata tcctatgcct
     6841 cttaatgttt ttatacaagg tggatttttt aatttttctc taagacgctt tattgtaaca
     6901 gtaagagtgt tatcatttac gaaattacca tctacatccc agagcatttc tagtaacttt
     6961 gtacgggtca tagctctatt tttattttcc ataagactta gaagaagttt atattctcct
     7021 gctgtcaaaa gtacttcaac attatctaaa tatgctttga cttcaacttt attcaagctt
     7081 atatttccac atgtaatagt attgttagta tttgaatgag ttcttctaag aactgcatta
     7141 attctggatt gaagtactga gagcagaaat ggtttaacca catagtcatc tgcacccata
     7201 tctaatccac gtataatgtc tttttcatca tcacggactg tcaaaaatat cactggagta
     7261 tcttctatac ttttaacata ttggcaaaac tcgtatccaa taccatcagg tagatttaaa
     7321 tcaagcagaa ttaaatcaat agttttatca aaattttttt tagcttcagc aatcgtatta
     7381 taacaacata cagtgtgatt tttcttttca agggaagttt ttattccaaa tgtaattgta
     7441 tcatcatctt ctaaaagaag tattttagcc atttactttt agtcctttca gtaatttagt
     7501 tatttcatta actaagttat tagttttatg tttaatattt tttatgttag agatagaagt
     7561 tagagataga aaaaattatc atttaagtat ataaacttta atttataaac ttaggtttat
     7621 gctttaattg aatctattaa aagagcaaga gttgtttcaa taaatttttc ctcatctata
     7681 ttaaagttgt tcaggtatga ctttcctgtt acagatagta aattaaaaaa tcctgtaagt
     7741 gtaaatattg atgaatacat gagagtaggt atttcaatat catgcctaat acttctatca
     7801 ttttttccaa tttcaaacat tccttgtatt tcatcaaaca taaatttatt aaaagaaaaa
     7861 aa
//
LOCUS       DAGQWR010000356         4446 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.guided.231, whole genome shotgun sequence.
ACCESSION   DAGQWR010000356 DAGQWR010000000
VERSION     DAGQWR010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.guided.231"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..497
                     /locus_tag="MIW95_004025"
     CDS             <1..497
                     /locus_tag="MIW95_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY3153263.1"
                     /translation="FEWVKLDSKEQVFHFDIFDLNELSRNIISDWIPVLESSHFEYEF
                     DIPEIEYFLRIDANAYTRIINNLLQNIITHSSGNKMTLRIFENKEQAQIIITDNGKGI
                     SSDNLPHIFERLYQCDHSRASKGNGLGLAIAKELINAHKGNITANSTPGMGTEFTILL
                     PKAL"
     gene            654..1577
                     /locus_tag="MIW95_004026"
     CDS             654..1577
                     /locus_tag="MIW95_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY3153264.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGCEKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            1570..2307
                     /locus_tag="MIW95_004027"
     CDS             1570..2307
                     /locus_tag="MIW95_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3153265.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFASIALGRRK"
     gene            2318..3091
                     /locus_tag="MIW95_004028"
     CDS             2318..3091
                     /locus_tag="MIW95_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY3153266.1"
                     /translation="MWKLLWAECQKIRRSKIVWITFFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            3107..3259
                     /locus_tag="MIW95_004029"
     CDS             3107..3259
                     /locus_tag="MIW95_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153267.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(3632..4240)
                     /locus_tag="MIW95_004030"
     CDS             complement(3632..4240)
                     /locus_tag="MIW95_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBY3153268.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         1498 a          680 c          754 g         1514 t
ORIGIN      
        1 tatttgaatg ggtaaaattg gattctaagg aacaagtttt tcattttgat atttttgact
       61 tgaatgaatt atcccgtaat attatatctg attggatacc tgttttagaa agcagtcatt
      121 ttgaatatga atttgatata ccagaaatag agtacttctt acgtatagac gcaaatgcat
      181 atacccgtat cattaataac ctattacaaa atattattac ccatagtagt ggcaacaaaa
      241 tgacattgag aatttttgaa aacaaagagc aagctcaaat tataataaca gataatggga
      301 aaggtatatc ttcagacaat ctcccacata tttttgaaag gttgtaccaa tgcgaccatt
      361 cacgagcttc aaagggaaat ggtttaggct tggcgattgc aaaggaactt attaacgctc
      421 ataagggaaa tattaccgcc aacagcactc ccggtatggg aacagaattt acaatattgc
      481 ttcctaaagc cctgtaaaag tacgaggctt tcttactcta aaacaagata aaagcaagat
      541 tatggcaagg ttttggcaag gttcatgttt tataattcta attgtagttg attttgacgt
      601 tgtatcaact atcttttgta gaaacaaatt tattaaaagt gaggaaatta tatatgaatg
      661 attttgtgat tgaaacaaag caacttacaa aaatttatgg gaatcaaaca gtagttgaca
      721 aagtcaatct ccatgtaaaa aaaggccgaa tatatggtct gttaggacgc aacggagctg
      781 ggaaaaccac aattatgaaa atgattttag gattgacttc aatcacgtat ggcgaggtag
      841 atgtatttgg agagaatatt aagggatgtg aaaaacgagt atatccccgt atcggtgcaa
      901 ttatcgaaac gccaggattt tatcctaatc tgacaggaac ggagaacttg gagatttttg
      961 caaaacttcg tggtacagcc gcccctaatt ctgtaaaaag agcattggag attgtaggct
     1021 taccttataa aaataaaaag ctgtttagta aatattcact tggcatgaaa cagcgacttg
     1081 gtattgccaa cgctatcctg catgacccgg aactgttagt tttagatgag ccaaccaatg
     1141 gacttgaccc tattggtatt gcggagatga gaaagtttat tagggacttg agcgttgagc
     1201 atggaaagac aattcttatc tccagtcata ttctttcaga aatctcattg ttggctgatg
     1261 atattggaat tatcgaccac ggtgttcttt tggaagaaag tagtatgagt gaactagaaa
     1321 agaaaaacag taaatatatc ctgctacaag tttcagatat tccaaagaca tcacttattt
     1381 tagagcgtca atttaacacg aaggattatt ccgttcaaga tgaccacact ttacgccttt
     1441 ataacactac tctggatatg ggtgaaatca ataaagcact tgtaatgcag aatgtaactg
     1501 ttattagttc tcaactttgt aatgatactt tagaggacta ctttaaaaaa atcacaggag
     1561 gagagggcat tgcttaaact tattcaaaca gaatttctta aactgcgtcg taggaaactt
     1621 atttggctga tgttgttgtc tgctctcatt atgccgtttt tcgcactact atacttcaat
     1681 tattttggta aaacaggtgt tgagccaaca aagttttata aatggtctgc atttggttat
     1741 acactttgga ttattttgcc cgttgttttg ggtatatttt gcaccatgct tatacacgaa
     1801 gaaaaccagt atgatatgct caaacagctt tggattgtac ctattagtaa aatgggatac
     1861 ttttttagta aatttttcgt tgttttgatt tactctattt gttttatgct gattacagcc
     1921 gttgcttctg tgttatttag tgtactttct ggatatgtcg tatttagctg ggcaagcatt
     1981 ttatatttgt tgaaaaaatg tttagaaatt ggtgtaatca ctgcttttgt aatgttgccg
     2041 attttggcaa tcgctacatc tcaaaaagga tacattctcc caatttgtat cacattggta
     2101 tatgcctttt ccggttttgt tataatgaca ataaatatgt atttacaccc attatccagt
     2161 atgggagtta ttatcatgag aaataaagac attccaggtc ttatattttc acaggaaatt
     2221 aatgtcccat tagcatttct ttgtatttgt atttggagta ttgtatcagt attgtttgca
     2281 agcattgcac taggaagaag aaagtgaggt gtagataatg tggaaattac tttgggctga
     2341 atgtcaaaaa attcgtcgtt caaaaattgt ttggattaca ttttttgcat ccattatgat
     2401 agcagtcata gtttttgcag gagggcagga tgtatataat gacccagatt tacattatgg
     2461 cttaaagagc gtttatgacg gttcacggta tatagacaat gcaggttggt atatggacgc
     2521 ggtacaacct tggtcaactt tttttgttct tcctgctgtc attgccctat taggaagcta
     2581 tattatttgt cgtgaagaag aggaggacac tattaaatcc ctgcgtctta ttcccataaa
     2641 tgaaacaaaa ttaaccatag caaaaatgat tattactttt atattcagta tacttcttta
     2701 tttgttactc tttactataa catttttgac tgaatccgtt ttacattttt ccgatttatc
     2761 gacaaaactg gttttaagtt gcatgaaaga gtattttctt gatggcatag gtgtattctt
     2821 tgcaatttct cccattattg ccttggtatc gcgcatgaaa aaaggatatt ggcttgcatt
     2881 agtattcaca gaagtttatt ctattgctgg actatttgcg ggtatgtcaa atgtcttaca
     2941 aacctttttt cctattgcag caatatttaa tatttctgga tactatataa ctactggaga
     3001 aaaaattatg gggagtatca taagtttatt actttgtgcc tgcctttctg catttatact
     3061 gaaagggcta aaacacaatg aaaaaaattg aaataggtat atcaaaatga aaaagataaa
     3121 aaaactgatt attctaattg tattcttgtt tctttttggc atgttacact gttatactga
     3181 atggaaattc aacaatgacc cacctaaagg ggtaatagaa agaataaatg agtggactga
     3241 taagattgga ctggattagt aacttatgtt aattatagtg aatgtaatat tagcaagtta
     3301 ttgtttcgta ttttaatact ataactttaa atatttggtt ttaatcttcc gaacgaatat
     3361 aaaaaaactg tcacagaggc aacatatttt tgaatattta taaagtggta gttttgattt
     3421 tcaaagctgc cgccttttct tctatataag caaacttatc ctcaacaaag tgtaaaacat
     3481 ggtacatttt aagaaaaaag taccacagac acaagcaata aaagaaacgc cgagaaccta
     3541 caaaaacgta gtgcttatgc agatttacga cgccttctat tttctcaacc gtcttaatga
     3601 ccttgtacaa aagaaatttt tctaatattt tttataatct tatcttgtga tgttttttat
     3661 gtataaatga cttaataaca aatccaataa aaattatgga tattggtata attagaacga
     3721 ggaatttata atcattataa tgtgacatta ttcttaccca actctcagaa aaaatatatc
     3781 ctatactgat taatgaagta ttccatatgg ttattcccac gatagatgat attacaaaag
     3841 ttataatatt ttgacctgct atacctgcta taaaagatat gtatgttcta cataaaggga
     3901 ttaatctaca tacacaaaca gaaatagaag agtatttatt aaaataatct tgagatgcaa
     3961 atattccttt ctttgccttt ggacatatct ctattatttt atttatgata gatttacctc
     4021 cccaatttcc cactgtataa caaaatatcg aaccaagtat acctgcaatt atacttaaaa
     4081 ttaaaattgt cagaaatcca aaatgatttc ttgcagcaat tgctccacat aaaggtaaaa
     4141 caacttcgct aggtaatgga aaacaagcat actcaatcat aattaataca aatacagaga
     4201 ttaatccata ttgtaaaata aaactattga ttacagacac ttacttatct atctcctttc
     4261 ttatttaaag gttttaaggg tacatattct aaggattctc accctatatt taaagaatca
     4321 atctctttaa actattatat gtatttacac aaagcattta taacatactt taaaaatagt
     4381 accttaaaat aagaaactta ataaataaaa aaagattacg tggctacgtc ctactctccc
     4441 aggcag
//
LOCUS       DAGQWR010000357         3195 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.guided.250, whole genome shotgun sequence.
ACCESSION   DAGQWR010000357 DAGQWR010000000
VERSION     DAGQWR010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.guided.250"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            <1..550
                     /locus_tag="MIW95_004031"
     CDS             <1..550
                     /locus_tag="MIW95_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY3153269.1"
                     /translation="KIKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEI
                     FSSENIEDIKFIKDGTKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINK
                     IYNRKIKIEREVNIFEDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDR
                     YKCCNVQYTKNRVIILYYIYNH"
     gene            complement(878..1645)
                     /locus_tag="MIW95_004032"
     CDS             complement(878..1645)
                     /locus_tag="MIW95_004032"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBY3153270.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKVAVMPQNERDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            2451..3083
                     /locus_tag="MIW95_004033"
     CDS             2451..3083
                     /locus_tag="MIW95_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBY3153271.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         1262 a          425 c          357 g         1151 t
ORIGIN      
        1 aaaaattaag atgtttaaat ataaaaatgg ctacatccct aataaattaa acctttcttt
       61 tggatatgga ttctcttctt ataaaaagca aataattaga gaaactgtag atactttaag
      121 attgacagaa attttttcaa gcgagaacat agaagatatt aaatttataa aagatggtac
      181 aaaaaaatta gaaattagca tagagaaagt tgtgaaattt aaacgtcgaa aaaaaagaaa
      241 ttatgtttgt tgctattgcc ctgatatgta tagagatata aaactcgaca aagaatctat
      301 caataagata tacaacagaa aaataaaaat agaaagagaa gttaatattt ttgaagatga
      361 ggatgttata ataaacaaga aagtattgaa gtttccaaag tcttggacaa agaatatgca
      421 aaaatattgg ttaagtgaaa ataagtatcc catacattct actgtaattg atgatgatag
      481 atataaatgt tgtaatgtac aatatacaaa aaatagagtg ataatattat attacatata
      541 taaccattaa tatacataat tacaaaaaaa taatgacaaa tatacataat acaaatatat
      601 cttctacttt agataaaaca gttattatga aaacttacta tctataaata atagaaatgc
      661 tagtatacca tctataatct aatgtctagt aggttttaat ggcatatttt aaatatgcca
      721 tagttgattt ataagatttt ctatatcact ttcaaaatac acactaaata gttgcttttc
      781 agatataatt gcccaattta aattttacta tctgaaaagt tttttatatt taatttatat
      841 cttcaaaaat accgtctaat atttctatat aattaattta attaatactc ttatgtaata
      901 gattaagtac tgaatttaac tctttaaatg atatttgccc tggtgctgaa acactttttg
      961 cagctccaaa agtcaatgca gaaccaaata tttcaccaca caatctactt ataacaccca
     1021 ttcctgacat tgacattgtt attattggtc tatctgcata aattttaaac atttcatttg
     1081 tagcttcaag tagcactaac acatctcttt cattttgcgg cataacagct actttgggta
     1141 aatctgcgcc caattcctgc attctacata aacgagatac aatctcttct ttttttggag
     1201 ttttattaaa atcatgatta gatataatta ctttcacttc ttttttatga gcaaaattaa
     1261 ctacttcatc tatgacctca tctcccataa ataactctac atctattaaa tctactaaac
     1321 ctgtattaga aatttcttta ttcaaagtag tataataatc tcttgatatt aatttttctc
     1381 ccccttctac tacacttcta aatgtgaata ataaagggat atcatgtata taacttctta
     1441 attcatagag aacttcctta acttctttaa tattttctac attctcaaaa aaatcaactc
     1501 gccactctat aatatccaaa catgcatctt ttaactcttt agcctctttt ataatatctt
     1561 ttttattttt cccaataatt ggtacacaaa ttttaggtct accttctcca atagtaatat
     1621 tttttacttg aacttttctt ttcatattct cctccataat cagaaattct ataggacttt
     1681 aaattctatt ttctgcataa tttaattata cattaaaaat actatacatt ataaaaatac
     1741 taatttaaaa ataattttta ttctaattct taaaacaatc ccacatattt tagaaaaggt
     1801 actctataat atacaaacac ttctttttta cctaaattta ttttaacatt ctataatttt
     1861 cacaatattc tttaaatttt actcatataa acaatattat tctggatgct aatagaatta
     1921 atctataaat catagtataa ttatgcagaa ataactcgaa tttcactact tataaagaga
     1981 ttgaatatta atgaatctaa atcatttgca ttacttcaga gttttgacta aagttgaaca
     2041 ccatacaaaa gtagctactc aattgtttat accccaacca agcttaagta atgttatata
     2101 gattcttgaa aaagaatttg gtatcttttt atttgaaaaa caaggtagaa atgtttccct
     2161 cactaaatac ggaagttttt ttctaaccta tgttgataaa gcattaggtt aattagaaaa
     2221 agtttacata taaaatatat taaattgttg caagttttat tgttttgata tacaatcaac
     2281 taaacatata atctttataa aatactatac agttaaaagt ctgttttaaa aaaaaatgta
     2341 taaattataa atcaaaatat agatactatc aaggagtgat tctgtgaaga gatgtattgt
     2401 agtagtggaa tagtcaaaaa cataatatat attaaaggag agatttaaat atgactattc
     2461 caaattcaaa taaggtatat ccaagaagta atgattatca aactgtatat cttaaaaatg
     2521 taataacaag ggaaaatata gaagttggag attacacaat atataatgac ttttataatg
     2581 acccaagaga ttttgagaaa aacaatgtat tatatcatta ccctataaac aatgataaat
     2641 taataattgg taagttttgc tcaatagcat gtaatgcaaa atttctaatg actagtggaa
     2701 atcactcaat gaaatcaaaa tccacatata cttttcccat cttttatgaa gaatggaaac
     2761 tcgatatcaa ccatataacg gacgcttggg acaataaggg taacattata attggaaatg
     2821 atgtgtggat aggttatgat tctataatta tgtcaggtgt aaatattggt gatggtgcaa
     2881 ttattggaac aagagcagta gttactaatg acgtctcacc gtattctatt gttggaggag
     2941 tacctgcaaa aattataaaa aagagattta ataatgatac aatttcaaaa ttattagaaa
     3001 ttaaatggtg ggattggcca tataaaaaaa ttcaatcaaa cattaagcat attcaatcag
     3061 gaaatattga taaattagtt taatatttta taatttattt atattaaact ttactttaat
     3121 aatcctttta taagagcttt ttcaaaattt gtatattatt ttaatatata taaatttaat
     3181 acaacattaa ataaa
//
LOCUS       DAGQWR010000358         1591 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.guided.295, whole genome shotgun sequence.
ACCESSION   DAGQWR010000358 DAGQWR010000000
VERSION     DAGQWR010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.guided.295"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            112..353
                     /locus_tag="MIW95_004034"
                     /pseudo
     CDS             112..353
                     /locus_tag="MIW95_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            402..485
                     /locus_tag="MIW95_004035"
     CDS             402..485
                     /locus_tag="MIW95_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBY3153272.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            610..1347
                     /gene="erm(B)"
                     /locus_tag="MIW95_004036"
     CDS             610..1347
                     /gene="erm(B)"
                     /locus_tag="MIW95_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBY3153273.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1493..>1591
                     /locus_tag="MIW95_004037"
     CDS             1493..>1591
                     /locus_tag="MIW95_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HBY3153274.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYP"
BASE COUNT          606 a          247 c          274 g          464 t
ORIGIN      
        1 gcttttatat attaatcaca aatcacttat cacaaatcac ttatcacaaa tcacaagtga
       61 tttgtgattg ttgatgataa aataagaata agaagaaata gaaagaagtg agtgattgtg
      121 ggaaatttag gcgcacaaaa agaaaaacga aatgatacac caatcagtgc aaaaaaagat
      181 ataatgggag ataagacggt tcgtgttcgt gctgacttgc accatatcat aaaaatcgaa
      241 acagcaaaga atggcggaaa cgtaaaagaa gttatggaaa taagacttag aagcaaactt
      301 aagagtgtgt tgatattgca gtatcttaaa attttgtgta taataggaat tgaagttaaa
      361 ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta ttccaaatgc
      421 gtaatgtaga taaaacatct actgttttga aacagactaa aaacagtgat tacgcagata
      481 aataaatacg ttagattaat tcctaccagt gactaatctt atgacttttt aaacagataa
      541 ctaaaattac aaacaaatcg tttaacttct gtatttattt acagatgtaa tcacttcagg
      601 agtgattaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag tgaaaaagta
      661 ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta cgaaattgga
      721 acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt aacgtctatt
      781 gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa cattcgtgtc
      841 actttaattc accaagatat tctacagttt caattcccta acaaacagag gtataaaatt
      901 gttgggagta ttccttacca tttaagcaca caaattatta aaaaagtggt ttttgaaagc
      961 catgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac cttggatatt
     1021 caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt gcttaagctg
     1081 ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg tcttaataaa acttacccgc
     1141 cataccacag atgttccaga taaatattgg aagctatata cgtactttgt ttcaaaatgg
     1201 gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc aatgaaacac
     1261 gccaaagtaa acaatttaag taccgttact tatgagcaag tattgtctat ttttaatagt
     1321 tatctattat ttaacgggag gaaataattc tatgagtcgc ttttttaaat ttggaaagtt
     1381 acacgttact aaagggaatg gagataaatt attagatata ctactgacag cttccaagaa
     1441 gctaaagagg tccctagcgc ctacggggaa tttgtatcga taaggggtac aaattcccac
     1501 taagcgctcg ggaccccttg taggaaaatg tcctaagtgt ggcaacaata ttgtattaaa
     1561 aaaatcgttt tatggttgtt caaattatcc t
//
LOCUS       DAGQWR010000359         1345 bp    DNA     linear   BCT 16-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT27 isolate 15.7921529
            SAMN25516633-rid13960973.guided.304, whole genome shotgun sequence.
ACCESSION   DAGQWR010000359 DAGQWR010000000
VERSION     DAGQWR010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516633
            Sequence Read Archive: SRR17631227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250361.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250361.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/16/2022 18:40:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,964
            Genes (coding)                    :: 3,916
            CDSs (with protein)               :: 3,916
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 26 of 48
            Pseudo Genes (incomplete)         :: 22 of 48
            Pseudo Genes (internal stop)      :: 9 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516633-rid13960973.guided.304"
                     /strain="RT27"
                     /isolate="15.7921529"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-05-28"
                     /collected_by="ModMedMicOxford"
     gene            95..793
                     /gene="vanR"
                     /locus_tag="MIW95_004038"
     CDS             95..793
                     /gene="vanR"
                     /locus_tag="MIW95_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBY3153275.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            829..>1345
                     /locus_tag="MIW95_004039"
     CDS             829..>1345
                     /locus_tag="MIW95_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY3153276.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLS"
BASE COUNT          512 a          155 c          217 g          461 t
ORIGIN      
        1 aatttcttaa taaattctaa tgtaaaaaat catataaaaa aattaaattt agtataatat
       61 atataaatat atatagaaag gatgtttaaa gtctatgagt ggaaaaattt tagttgtaga
      121 tgacgaacgt gatatagctg atttaataga agtctacctt acaaatgaaa actatattgt
      181 ttataaattt tattctggtc ttgatgcact aaactgcatt aatttaaatg aatttgacct
      241 tgcaatttta gatgttatgt tgccaggagt aaatggattt tctttatgcc aaaaaatccg
      301 agaaaaatat acttatccaa tcattatgtt aacagctaag gatgaagaaa cagacaagat
      361 tacaggctta actttaggtg cagatgatta tgttactaaa cctttccgac ctttggaact
      421 tgttgctaga gtgaaggcac agattagaag atataagaaa tacagtggaa gtttacaatc
      481 tggtgaagaa ctttcacata ttttttcttt tttaggtctg gaattgaata cccaaacata
      541 tgaatgccat ttaaatggta aagctctttc acttactcca acagagtttt ctattctaca
      601 tgtattattg aaaaataaag gctctgtggt aaattcagaa aatctttttc atgctatctg
      661 gaaagatgaa tattacaata aaagtaacaa tactataaca gttcatattc gccatcttcg
      721 tgaaaaatta ggtgatactg tagatgaagc aaaatatata aaaacaattt ggggtgttgg
      781 ttacaagatt tgacacctat ttttcttgtc aagaagggat gtagtattat gaaaaagatt
      841 aataatttta cttataggaa actaagagat aaatataaat ttataaaaag agaaaaaaat
      901 acagaggcgt atttaaagta taaattatat ataatgttag ctatctatac aggtatagta
      961 ttggttttaa tgtttatggc tgattatacg ttttcaaatt tttcaaatcc tcttgttaaa
     1021 tggcttaagt tgagaagaga tgtattattt tttttggttt ttataattgg atatatcact
     1081 attttcaatt tttattggaa aaaaccatgg aattatttaa gagaaatcat caatgcaaca
     1141 catactattt atcaacaaaa tgatgaattg gttgaactat ctaagccatt aaaggaaata
     1201 gagcaacaaa tgaaccaaat taagatgtct gttttgttaa gtcaacaagc agtgaaagag
     1261 gctgaaaata aaaagaataa ccttgtgatg tatctagcac atgatataag gacaccactt
     1321 acttcagtta ttgggtattt gagct
//
